LOCUS       DAOTPW010000001        62383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_520_length_62383_cov_6.128947, whole genome shotgun sequence.
ACCESSION   DAOTPW010000001 DAOTPW010000000
VERSION     DAOTPW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 62383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62383
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_520_length_62383_cov_6.128947"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1557)
                     /locus_tag="P5316_00005"
     CDS             complement(16..1557)
                     /locus_tag="P5316_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRR83360.1"
                     /translation="MQFIVFSAFAIVLSIPFEGPARAWVLVSSPAWIFAAVAGHILAA
                     GLAGAITTRRVRAKLEREPSWLPAAQRRLGHGHTVIRAVLLLSFGSLVFLTDWLRLIR
                     SWDGIAAVWGLDEIIAIAPFFAAILASYVAVYPADRAIRQVAMELRLWASVPTRPPWS
                     LRAFVRFMFRQNVLIIAVPMLPIMVANDFVQCYAAPIRKAAFGIVWADQVVLVAIAGL
                     VFLVAPVMLRYIWHTRVLPDGELRRRLEGLCRRVGLRYRRILIWESDGMVVNAAVMGL
                     IKPVRYILLSDGLLEMMDDAKIEAVFGHEAGHVKCRHIEFYLLFAVLSMLIVGGVMEL
                     IAFAASKWPTFFDQIADLEAYLPLLATVMILLIWLLGFGAVSRQFELQADLFGARNVT
                     PGGEGCHLPCFVHRPPPISADRSNPASAQAICASAAELFADALYRIAALNGIPIDAKS
                     WRHSSIANRMEQLRDYAVRPGAAAWLDKKVLLIKGTLLGGTAVGLVVALLIYSPQLRA
                     WCWGR"
     gene            complement(1644..2645)
                     /gene="fmt"
                     /locus_tag="P5316_00010"
     CDS             complement(1644..2645)
                     /gene="fmt"
                     /locus_tag="P5316_00010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRR83361.1"
                     /translation="MGRGRAIGAACDPHRSGLMRVVYFGSGEFAVPALRWLAHSPHEI
                     AAVVTQPDRPAGRGKKQTATPVGGMAESLGLPVYKTEDTNEAGFVEQIRSLGADLGIV
                     AAFGQKLHAPLRSAFAGECINIHSSLLPKYRGAAPINWAILKGEPKTGVSVFRLVDRL
                     DAGPVLIRRETMIGSTETAAELHDRLAGIACDALGATLKLLEQDLHHPGEPQDESLAT
                     QAPKLTKADGMLRFDEPAEQIALRCRAMWSWPGARCLYRNADGRTEEIIIATASAIPA
                     QASEPPGTITSLLTVATRQGTLEIHGLKPAGKRVMSWQDFVNGRHVKPGDRLEAVGP"
     gene            complement(2930..3496)
                     /locus_tag="P5316_00015"
     CDS             complement(2930..3496)
                     /locus_tag="P5316_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83362.1"
                     /translation="MNSHIAPLEAYMGRQIRQHQKADKPAGRTQSGLPVWLPLVGGLL
                     AIGIVIAMAAYVLNKDGSAPQYTPPAAQETKAPARPAPAKTVDTGKLVGRWLRTDGNY
                     VLEIRSVDTAGKVNAAYFNPGPVNVSKAEVASDKGETQLVVELKDRGYDGNYYTLAYD
                     PITDRLTGVYHQLTMGQQFDVEFVRMPR"
     gene            complement(3493..3627)
                     /locus_tag="P5316_00020"
     CDS             complement(3493..3627)
                     /locus_tag="P5316_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83363.1"
                     /translation="MTPDHEVEHESIFSSQARRFCDARRAARSSLTGALNAAIMRTMM
                     "
     gene            3798..4148
                     /locus_tag="P5316_00025"
     CDS             3798..4148
                     /locus_tag="P5316_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83364.1"
                     /translation="MGHDTEHRADQLSPISRRAFFQSLGNKALKLGIFGTAAVSLGSD
                     DGGCRIKIGYVDANCGYCDGAGQDYEHCDGDYCDYANYGDYSDEGCGYCDGTGQDYEH
                     CDGDYCDYANYADA"
     gene            4103..6421
                     /locus_tag="P5316_00030"
     CDS             4103..6421
                     /locus_tag="P5316_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR83365.1"
                     /translation="MRRRLLRLRQLRRRVTAKPGVLSVMEIIIKPTEACNGTCVYCSA
                     DGTLGKRKILPCEKLGPLFDIFAKWVRRDDRRNLRFLWHGGEPMLCGPEYYAEIMQQQ
                     QRAFGDDLDRVRNAMQSNLSLVDEHWVPVLRDLLKDQAIGTSFDIVDGVRGLAGGKNL
                     REIWVRAVRLLRSAGIRVGAVYVAHRQSLDRVGNIYNFFRNAGLHVRVNPLYKEGRAD
                     RRQSQPLWITADEYGQFLVDLCDLWRADRMRWSIMPLMEWYRAWKGHSRLCCDSRGMC
                     HTSHLGINPDGAVFGCGRQSDHGANRLGNIFEDDLDEMLRQREQGPLAGRSSSLRDGF
                     CRDCRFWKFCHGGCPMMGLLYHGDMMRETYFCAARKRVFEHFEKLFGPPTVSDQPSAL
                     SGQPLAPRRGSGPAFSGQQSAISTQQEGTQQEGAQQEGAHQKRPDLPPLPRGGRGGWE
                     CPTSSGGDGASSLNPACHAEAKRRREPRLPGEAVAKPGTLNPLSRFVVTIERPDGWPM
                     LDFVRIVTPQLDLERLALGRLDPQERARGLVNLEHFLASPSGYKPFARQLTIMAGPEF
                     AYSQNKADAMQDGLLGTLRRLRAELMFSSATIGDGTFARRAADASVPVRLEFGTDYDE
                     AVLSGLLDHFLHTPTLQVPIEPFFTLAGAIGGQRRLTLWQIFDNLTGRCLFVDAQQRV
                     SVSRWYAHRGAFLGKVGDSIKVMRESAAWKQIEAYAERVFIEQTPCAFCEHYPYCGGI
                     WRATSEADTACLVWRRLMDRMVKAYRGCLPAT"
     gene            complement(6432..7520)
                     /locus_tag="P5316_00035"
     CDS             complement(6432..7520)
                     /locus_tag="P5316_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRR83366.1"
                     /translation="MSGLNVRKDPCELDFLALGAVVHRLDPGVVPFRKARSVDIHVSG
                     GEYNVAANLADCFKLRTGIATAMVNYGIGELVQGRIREMGVTPFYKWFEHDGVRGPNI
                     ATVYSDRGYGVRPPVVFYNRSNEAGSLLKPGDFDWADIFARGVRWFHSGGIFAALSET
                     TSELIIEGMEAARKSGAVNSFDLNYRAKLWAPIGGLEKAQKTLRKIVGNVDALIGNEE
                     DLQKGLGIAGPEVAHKAASKLDPETFFQMIERVVEQFPNVKMVATTLREVHSTNRHDW
                     AAVLWLDGKRFVSPTCQLDVLDRIGGGDGFAAGLIYGLLAGREPEQALRLGWAHGALL
                     TTFPGDVTMARLEEVEAFAKGGSARVQR"
     gene            complement(7702..8016)
                     /locus_tag="P5316_00040"
     CDS             complement(7702..8016)
                     /locus_tag="P5316_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83367.1"
                     /translation="MSSMGPQFGVGSALIILMLSACCLPAAEAAEYSTPRQLSIFDVY
                     QLNPDGATGDEATTEPKTDLNNGLNGDNPYFQRTKCGQSTRRRLLWKILVHIVLPGTG
                     RA"
     gene            complement(8075..10162)
                     /locus_tag="P5316_00045"
     CDS             complement(8075..10162)
                     /locus_tag="P5316_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HRR83368.1"
                     /translation="MNGCVSSGRRRYSGRVTLGVALWLLAGSGARAGLIGTPPSTATT
                     FRVCELAFSADAPGSNPYLNGPSVTATFTGTSGPASGKVLTMKGFWDGGNVWRVRFAP
                     TAQGDWSWTTSSTDAGMNGFSGTLTAIGPTPEELAANVLYRGFLHRDGHAWKLSDGTP
                     FLAVGDTQWSFAEENTTAEWQQWMNARQAQNFNTFLGCIWLAIYTRSGVPDAFPSKNP
                     QTDTPNMAYFQRLDQMVQYANDRGIMMGLTIGGFPDNSNWWVKFGTLERNSRWFRYCV
                     ARYTAYNVRWCLYGEVNERNTPWGSTWQQQVAYDAQLVRDEDPYDHPIGSHHTSVDTS
                     SANSPNIDYIEVQIARTETQYQSALNYRQYGKPVWFEEYWYEPATYDNDVVLGIRNTH
                     RNFVAAMAYPTMGSLMRAHYPDFNINDVSTDPGAVRMSYFWSFYKDLAFLSFSPASGL
                     VSRGQCGRFGNDYAIFLQGGGSVTLNLTGVSGVFDVTRLDINTGVTADLGLITGDGQR
                     TINSGTTSDVAIRVVKTPGPNQPPVVSAGDDQVVHLSQGAVLDGTVSDDGQPDPPGVV
                     TTIWTQVDGPGVVTFADAASVDTTAGFSQAGVYTLRLTANDGELSASDETVITVNGGG
                     GCDFDGDTDVDQVDFAHLQACATGSGQAVTNPACFNAVLDGDEDIDQNDFAVFVGCVS
                     GPDVPATAECRGE"
     gene            11033..11530
                     /locus_tag="P5316_00050"
     CDS             11033..11530
                     /locus_tag="P5316_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HRR83369.1"
                     /translation="MGQTQQDDIDALLAGVSQLAADAAADIVGDDGTGPISSGPATAV
                     ANAPAAEDGGKRVPKRTQMPSGMPTVAPASSERSEDPQRILPLEVPVIVTLAERTMPL
                     SKILALTPGSIIEFDKPSDELLDLMINNRRIGRGQAVKVGENFGLRVTMIGSIGNRVE
                     AMNAV"
     gene            11626..12285
                     /locus_tag="P5316_00055"
     CDS             11626..12285
                     /locus_tag="P5316_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRR83370.1"
                     /translation="MSGTYSEFSVFRGHIGQRIRYYRQRLGLNQTQLARRANVNQGFL
                     SEIERGKRKPSPSSLRAIAEALDVAPAVLIGDGLEHDTPQPLETRDLPLFGAIPAGSP
                     AETQEQIEMFPVLRHLWGHDRYILRLTFDSMEPTLKPGDLVLVEYRQDVNPEHVQGRI
                     CACLVDGTPTLKRVSVELQRSSRIVILRGDNPTVPPMLIDESRDFLIQGIVTHLVSRD
                     L"
     gene            12596..12853
                     /locus_tag="P5316_00060"
     CDS             12596..12853
                     /locus_tag="P5316_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83371.1"
                     /translation="MMTGSGTKTRRLCSFRLADVLCPEPRQVLVHLTPELEVSGEVVF
                     MSDQGQQPDRFAIVNVEGILSPLIVPTERLRGEEHPEARGY"
     gene            13789..14151
                     /locus_tag="P5316_00065"
     CDS             13789..14151
                     /locus_tag="P5316_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRR83372.1"
                     /translation="MQTITKKELIDRIAESCNHKRVVVKRIIQTFLDQIIDELGRGNR
                     LEFRDFGVFESKVRAARTAQNPKTLERVAVPSKRTVKFKVGRLMKDRLVAASGDSPSP
                     LSGVAGRKPASGNDGSKT"
     gene            14418..15290
                     /locus_tag="P5316_00070"
     CDS             14418..15290
                     /locus_tag="P5316_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83373.1"
                     /translation="MPDERLRHGWLRALGRAEMPARIALHDGYYTHLRTWKHDFFAAT
                     GLYEGPSGRVVLKLGRTASLLVIPGSWIGRLLCDRELDLYRAVDDLPGVPKCLGKWGR
                     TGLVHAFVEGHPLQKQERVDDEFFPRLERLINELHRRNIAYVDLEKRENILVGAEGRP
                     WLIDFQISWRWPSDAAERRGGLKRLIPDRLGLYLLKRLQEGDRYHLLKHRRRHRPDTL
                     TPEQIEASYRLGFWHRMHRRVSRPLTLLRRSVLKMLTGRWQSLKQDGPEFMEQPAAGA
                     AGRMSAETRNSGTA"
     gene            15342..15839
                     /locus_tag="P5316_00075"
     CDS             15342..15839
                     /locus_tag="P5316_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83374.1"
                     /translation="MRSSLTRQELDLIEAFRSRVETLAGTRLGLPQLERHDRPDHWVM
                     ASRWPMDRHWWLELAVRPTVPQIRVTVVTDDPMRSLDAEVMISESSLTLREFISLGFH
                     DAGLAWPEPPVEHYRENAERFCFATPLDLASLDELCGEAMREKVLKMLDGYHRCFTGR
                     VVGSR"
     gene            15887..16990
                     /locus_tag="P5316_00080"
     CDS             15887..16990
                     /locus_tag="P5316_00080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR83375.1"
                     /translation="MADDAEQIISPVSTLLVIDDDVLDRLGSTVRQLCVGMIDEVVRV
                     MVLARSTCRIEEAIGPSQLFYLPRRCWPWHWRRLNPDQALALIGDEKPDVIHCMSADL
                     LHMAQDWVMEWGSRVVVHLTDLSDVRRFLRLRLPPELVVICTTPTLQQSLVRKRPDLV
                     SRIRVIPLGIPASPEPACFADPERVPTVLVTAPLTHDCGLHLILKAMDAVIAAGQDMH
                     LFVLSSGPGEYVFRKVVRQLGLRSRVTFAGELHDWTAMQAAMRDADFYILPGERRRFT
                     ISTLMAMATGLAIIAPTGTIGDYLIDGVTASLFDPQKPRQISEKLSALLADKAVARRL
                     AQGGLEYIRAHHQASAMVSSIAGVYRELCSPGE"
     gene            17252..18979
                     /locus_tag="P5316_00085"
     CDS             17252..18979
                     /locus_tag="P5316_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRR83376.1"
                     /translation="MSATLPFIPDLRILRPGVRSLLLVAMTALAAVPFRDSSAQETNA
                     TQPSASSLTSQQAGQIYVLPIRGEITNVTHDSLKRRLGMTEGTNLQIVVIELDTPGGA
                     LKATLEICHELKALRDDGVRVVAWVNHEAYSAGTIIALAADEIVMARNATIGDCQPIQ
                     ITQEGASAIPDDIKAKAVSPLLAELEDSARRNNYEYETLLALIDPAVSLYWLRNTETG
                     ERRLVDTKGRDRLFGLERKESAGGGVSGLLKILGTNTEKDTKDSSGDVSDSRSRTAWK
                     YVENDPSLGRISQPVDGPDQLLTMRTDKAKAFGFCRATVSNDAELREYLKVNWPVTRL
                     ESNWIESIIKWLASPLVRGILFLFMLLAIYTELNTPGVGIAGAVALICLILFLGAPYL
                     TGYTVTWEIVAIVLGAILLLIEIFVIPGFGVAGVLGVILMAVGLLFSFAPSEPGFERE
                     LWPRIPTFGMIYVYLKHGLYALTGGLVGSLVGMYFIARYLPRVPVAGSLIAPNPDHEA
                     VQLDDPYHGVALVGDTGTAETLLRPAGKARFGRMLVDVVSEGEYIESGAAVVVVERRG
                     NRVVVRRAG"
     gene            19101..19586
                     /locus_tag="P5316_00090"
     CDS             19101..19586
                     /locus_tag="P5316_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83377.1"
                     /translation="MPDWLGIVLLYLIGTAVLVAEIFIPSHGVLSLVAFAMLGFAVYE
                     TFQMSGLWGLVGLSSLAVLIPASVLMVVRTWHRTPIGKRICPPNPQLSEEDRMPLEPL
                     RAVIGRRGRTVTLLRPVGTCDFQGRRYECKAEQGVIQKDVEVEAIGLVDRTLVVRPVS
                     S"
     gene            19766..20833
                     /gene="floA"
                     /locus_tag="P5316_00095"
     CDS             19766..20833
                     /gene="floA"
                     /locus_tag="P5316_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327694.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRR83378.1"
                     /translation="MIEHILALSLLAQRTPQHDGIPWGIIVIVAIGLLCLFVLALVGQ
                     VFNLWLQAFMSGAGVRITDLIGMRFRKVDARTIVMSKIRAVKAGLLEITTNDLETHYL
                     AGGRVQNVISSLIAADRARIDMTFKTACAIDLAGRDVLEAVHTSVNPKVIDCPNPASG
                     KQSIEAVAKDGIQLKARARVTVRTNIARLVGGATEETIIARVGEGIVTSIGSAENYKV
                     VLENPDVISKRVLEKGLDAGTAFEILSIDIADVDVGVNVGAQLQADQAEADKRRFQAE
                     AEKRAAMARAREAEMRALVEENRAKVVEAEATVPLAIAEAFRSGNLGIMDYYRIRNIQ
                     ADTAMRSAIGGETKPPEQKKS"
     gene            20860..21516
                     /locus_tag="P5316_00100"
     CDS             20860..21516
                     /locus_tag="P5316_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83379.1"
                     /translation="MWQHVTIAEAGSSCFFYVLLVVVLIVMGYLRMKIEQVGGEQRKA
                     GPKRPVQRGVPQSRVEEPGRRLPPRPPAPGRPLQPQRQAPRPPAVWEAPAPRRPDQPQ
                     PVARPQPSPQRPSSPTPRPPQERRVVAAAIPDQGEPAAKEFTRIASKPLPVVAPPEAK
                     TLAVPAVGESELQPSTSVEARRPFVRLVGLPLIELQRAFVFGEILAPPLALREEQKLF
                     "
     gene            complement(21580..24369)
                     /locus_tag="P5316_00105"
     CDS             complement(21580..24369)
                     /locus_tag="P5316_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83380.1"
                     /translation="MKNSKSILASVLLFCLGPVLVTGCAGPQRFRKPAPAEDGVSTIP
                     EPVLGFAAGQEDGARQLHVMEWVAKRAWYNLGLPFERADFKGPPEQKVRVNDSWTEIT
                     NTVACGGVPLRNQIVYFEIEQRDKPIIIVLRSTDAFGEVMIELDLLKGTARALSITVS
                     RSMIGGQSHSSPMPEESNACLIGEFQFRRLARGWHLLSLEARGARVTLRAAPDIWLGF
                     RVAVPLSSWSNLLEFEDPDPAGGKYGFGSTGTVAVRNISQYELISSAEKARREACLRD
                     MHEFCKGLDAEYEGDVRKANQVEVTPDGVKWTWPPTGATAVVSADRGVPHGVVKAGLY
                     GNDTLIEGAFPEVIVTSEDGEAFRADPDGQVAFQADPLALRMALPLVSGSGRKATAHV
                     TARFTVLTVWWWTVTVEGVEPKQIQAFVGLAPQFAARTKQAASSEAGMAVAIASGMKG
                     GAYYEHNAKAGVLIKSLAPDIDLGSRAGGKGEVALVIKGSKLRFATLWLPAQPLNRIG
                     FTTRMVHYIKYPEGPIQHWRRMPSFQEYPDNVDLARFKANGTEAMVWHHTWISNDFRD
                     REGFLVNHREMKRAMRETHRLGMAAIGYIGIVPGRSSLLRFDDALDDYQKNWDLQDFT
                     FYATPGRWQEFLPWMTDYWCREYGLDGFYADGGLGGATWGRLRPEKGTGPICRDGPEG
                     ASQKSGLFPFPPEDADLSLDEIQHRFYYRIKKVLQRHKARFGLEQWGGSHDMLITGFY
                     DCRMIGESFQEAPPEDYRNSYNALLTGTPFKMYGMRETSQNPYNIAMAAVCMSDIQVC
                     SGNGAWGDVADTAETWKRIRPLWRLLESIDFDRLIEARPWYAQELVSGEGFYAGNYTM
                     PNRALVFLANKSETPGPFDVQIKVDHLPKINGQWHVRYVLGRQGALGPLGDGRIKLTL
                     PGLHSGPIGLELIAR"
     gene            24542..25957
                     /gene="bioA"
                     /locus_tag="P5316_00110"
     CDS             24542..25957
                     /gene="bioA"
                     /locus_tag="P5316_00110"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRR83381.1"
                     /translation="MPAKSDSQNLPPDQKHQLTEWDRRCVWHPFTQHSLWNVAEPLII
                     VGGEGEYLLDADGNRYIDGHSSLWCNVHGHRRPEINGAIIKQLDRIAHSTQLGLASPP
                     AIRLAKRLIDIAPPGLTKVFYSDDGSTSVEVAAKMAYGYWHHNGRPQRSRFIALRNAY
                     HGDTIGAVSLGGIDLFHRVYSPMLFKTEFAPSPYCYRCPLGKCRDTCNLACADEIGRL
                     LSEYRGQVAAVVVEPLIQCAAGMITAPQGHLRRVRELCDRHDVLLIADEVATGMGRTG
                     RMFACEHENVAPDLMCVSKGLTGGYLPLAATLASERIYEAFLGPIDSGRTFYHGHTFT
                     GNPLGCVAALANLDIFESDRTLEQLPPKIERLAEHLRRLADNPHVGDVRQCGMIAGIE
                     LVKDKSTREQYPYGMQVGAQVCAHARQYGVIIRPLADVIVIMPPLVISAENIDHLMNT
                     VERCITEVVPRVQTGVGDGLE"
     gene            26063..27049
                     /locus_tag="P5316_00115"
     CDS             26063..27049
                     /locus_tag="P5316_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRR83382.1"
                     /translation="MALNLTPDYFEADRKYRQARTPAEKLAALEEMLRTIPKHKASEK
                     KQADLKRRISELRQGELQRARKSTGVDPYHIERQGAGQAILIGPPNSGKSSIVGTLTK
                     APVKITDFPYGTPIPVPGMAHFEDVPIELVDMPPVTPDHLPGGLVNAIRNADAALMVV
                     DLTAESVLEDIDGLLAALAAKEIEFGRSEADGEEEMRVGPHGIILCNKMDMPGAVDTF
                     QMLKELRPAEPEMMMVSGRTGENLDVLMRKLFGLLDVIRIYSKEPGRPVDRDRPFILP
                     AGSTVEELARHIHKDLAANLKYARVWGEHTYDGQQVHGRYTLHDRDIVEIHA"
     gene            complement(27040..29607)
                     /locus_tag="P5316_00120"
     CDS             complement(27040..29607)
                     /locus_tag="P5316_00120"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRR83383.1"
                     /translation="MGQQVSTRKLQIKVAAAIQQGQERIAGRSKLIDRALQHLRLSQG
                     KHWEGATPHDKYVSLALAVRDLAVERMIQTQAAYAKQDVKRVYYLSLEFLLGRLLRNN
                     LVNLGLYGECCSVVSEDSDFDHIFDVEPDAGLGNGGLGRLAACYMDSAASQGYPVYGY
                     SIRYEHGMFHQEIENGWQVERPEYWLRFGTPWELMRPEFATIVRLRGHVEHRTDSQGR
                     YRPAWVGYHTVVGIPYDIPMVGYGGRAVNILRLWASSASEMLNLEAFNQGGYLEAVRE
                     KVMNETISKVLYPADQTQRGRHLRLVQQYFFVACTLADILRRYDRDHDTIDDLPNKVA
                     IHLNDTHPSIAIAEMMRILVDDRGLDWDRAWEMTQAIFAYTNHTLLPEALETWPVWMF
                     EQWLPRHLQIIYEINKRFLEQVDRRWPGDDGRKRRMSLIQEDNPRSIRMAHLAIVGSH
                     TVNGVAELHSRLIRQTLVPDFAELWPDRFTNVTNGITPRRWLKAANPGLAAAITRRLG
                     EDWVCDLNQLKGLTRYADDPEFQDEFRQVKQANKRRLCEHVLRTAGVNLLVDSLFDVQ
                     SKRLHEYKRQLLNILHVVMLYHEMLSRPQARVPRAFIFAAKAAPSYHRAKLIIKLIHD
                     VARTINADKRLDGRLRVVFVPDYCVSVAEIMVPAADLSEQISTAGMEASGTGNMKFAL
                     NGALTIGTLDGANIEIREAVGAENFFLFGLTAEEVAELRPRYNPWEVYHSNPAVRQAL
                     DGVADGEFNPEQPHLFKQVRDWLTSEGDRYMLLADIESYAEAQRRVDALWRDPAAWTR
                     KAILNVANMGKFSSDRSVNEYAQRIWNTSPVRIDRIPIDAQTSGVAAESGCSPFSHA"
     gene            29904..31628
                     /gene="recJ"
                     /locus_tag="P5316_00125"
     CDS             29904..31628
                     /gene="recJ"
                     /locus_tag="P5316_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRR83384.1"
                     /translation="MPKNWTIIPAWPECAAAARRLGIHPLLAQVLHNRGVADPDAARL
                     FLNPELKCLLAPETLPGAVEAATIIARKIRERRPIVIYGDYDVDGITATAILWHLLTL
                     AGARVCFYVPNRLEEGYGVNAEALRQIRQDGADTVITVDCGITAVKEAEIAREIGLTL
                     IITDHHSPGATLPEADAIVHPTAAGAYANPDICGAGVAFKLAWAVARMLSQSERVKPE
                     FRKFLIDATSLAALGTIADVVPLSGENRILARFGLLGVNDTRLIGLRALIESARLSDQ
                     KIDSEHVGFWLAPRLNAAGRMGHARMAVELLTLADAPRAREIAAYLESQNRGRQAVER
                     QILKEACGMIDAGNLAGDARRAIVLACEKWHAGVIGIVASRIVDRYCRPTVLISLENG
                     QGQGSARSIRNFKMHQALAECSDHLEAFGGHAMAAGLRIRHENIESFTERFVELANRT
                     LTCKDLEPSLRLDAEIGLNDLPDALVHELQRLQPFGVGNPKPKFASGILQLAGEPRLV
                     GKNGQHLQFALTDGRITRKAIAFNQKDALRPLLDHRRCRVAFEPILDTYNGRTSVKLQ
                     VIDMAFPE"
     gene            complement(31844..35314)
                     /gene="metH"
                     /locus_tag="P5316_00130"
     CDS             complement(31844..35314)
                     /gene="metH"
                     /locus_tag="P5316_00130"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRR83385.1"
                     /translation="MDSAFLRALDERIMVLDGAMGTSVHAFDLPLSDYRGLENCTEIL
                     CLTRPDVVCEIHARFLGVGCDAVETNTFGANKIVLAEFGLAGQTRELNRAAVRIAREA
                     CRRFSAPDHPRFVIGSIGPGTKLPSLRQVTFDELEDSYAEQIRGLMDEGVDALLFETC
                     QDILQAKAGLAAAEIVFAECGRRVPVMVQVTMETTGTMLVGCDIGAALTVLDAYPQIE
                     VIGLNCATGPEQMSEHIRYLSEHCTRRISVVPNAGLPELVDGKPRYPLSPEDLTRRLV
                     EFVEQDGVNMVGGCCGTTPEHMAQVVKAMKGRRPAPRSPRFEPSVSSLYQSITIRQDN
                     SFLIIGERTNANGSKKFREMLAAEDFDGMVEMAKDQVRAGSHMIDVCTAYVGRDEMAD
                     MTRLIARLATEVPVPLMIDSTEASVIEAALRLVGGRCIVNSVNLEDGEKRCAEILPIC
                     RKYGAAVVALAIDEEGMAKTADRKVGIARRLHDLATGKYGLRSSDLLFDPLTFTICTG
                     NAEDRRLGLETLEALRRIKQELPDCHTVLGVSNISFGIKPAARQVLNSVFLHYARERG
                     LDAAIVHAGGFQPLYKIDPKLREIARRLIFDDRQEGYDPLADLLAQFGDDAVSRPEAA
                     ARPPATIEERLKQRIIDGDRTGLTDDLDEAMKTYKPLQIINELLLPGMKAVGELFGEG
                     QMQLPFVLQSAETMKTAVAHLEPFMERTDATGRGSIVLATVKGDVHDIGKNLVDILLT
                     NNGFKVHNLGIKVPIKAMIDAWQEHKAQVIGMSGLLVKSTLVMRDNLLVLNERGLTPT
                     VILGGAALTRSYVERELRPLYRGSLFYAKDAFEGLGLMRRICEQGVQSIPVQTAGGSD
                     PGEYEMGSGCARSSTPRPAVLRDDRIERSGIATDVSIPTPPFWGAQVIENIPLTEALS
                     FINEFMLFQSQWQFKKRGRPQEEFDRFLDETVRPIYRDLVARCNAENILQPKVIYGYW
                     PCQSEGNSLIIYDPQDRDRELVRFDFPRQQKPPWWCLSDFWRPKSTGQYDVIGMSVVT
                     MGPHASEVAREWFEAGRYTDYLYLHGLSVEGAESLAEWTHKRVRVELGIAGRDAPELR
                     KLLQQGYQGSRYSFGYPACPNLEDQVKLWRILHPERIGVRLTEQLQMVPEQSVSAIIA
                     HHPEARYFKA"
     gene            35553..36590
                     /locus_tag="P5316_00135"
     CDS             35553..36590
                     /locus_tag="P5316_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83386.1"
                     /translation="MAKTWKAGVIGCGSVAQHLHLPGYMRTPGVELVAACDPEPARHK
                     EVRQIKKNLHLYRDYREMLAREELDVISVASPNRFHAEHAVAALEHGAHVLLEKPAAL
                     SMREIAAIRKALAKSGRQLIVGFSQRFSRGNRKLNALVRGGVIGEPYMIRIRFAHSGP
                     YPGWAKDPWFYSPVKAGGGALLDMGIHAIDQAQWHIGPVRSVQAHTATLRKKIKVEDN
                     AVLLLEFAGGKALGYIEVGWTSPSGFNGIEVMGDEGCIITDYTGVMRLTTGKVTPDTK
                     ARLKLKTRVIDKAPTTGGWSVEIQEVVKAFRRNSNLGSGIDAGGSALAVALAAYESSR
                     TGKRAKVASIR"
     gene            complement(36757..37386)
                     /gene="purN"
                     /locus_tag="P5316_00140"
     CDS             complement(36757..37386)
                     /gene="purN"
                     /locus_tag="P5316_00140"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRR83387.1"
                     /translation="MGNANQKLRLGVLISGSGRTLQNFIELIKAGQLPAEVAVVISSL
                     PDVKGVERARAAGLPVVVIRKKEHPDEAEFSRLVVEALEAHDVQLACQAGWTCYWRIP
                     DRWLGRVMNIHPALLPKHGGKGFYGHHVHEAVLASGDKESGCTVHFANNEYDAGPIIL
                     QRKVAVLPGDDADTLAARVFEQEILAYPEAIRLYAEGRLRLVGDRVEIR"
     gene            complement(37438..37863)
                     /locus_tag="P5316_00145"
     CDS             complement(37438..37863)
                     /locus_tag="P5316_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83388.1"
                     /translation="MAYFREFLGEDTILITGEAADLLVLAKRLRVLEDPASQPIPIHA
                     LPFVTAQGGLQLTAHPVGQDLVLRLRENAGGLLCFDWQYSEDGWLEVVEKIEHLATSG
                     KAGHNWMNGDYADDAVVMVSRGEYDDCRWENAYSRQARG"
     gene            complement(38010..38198)
                     /locus_tag="P5316_00150"
     CDS             complement(38010..38198)
                     /locus_tag="P5316_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stability protein"
                     /protein_id="HRR83389.1"
                     /translation="MAGLLLKNVPPEVHTRLKRRAARTRRSLNQEATAILKSALRDET
                     KRPTLEEIRKWQVRGASR"
     gene            complement(38275..41580)
                     /gene="ileS"
                     /locus_tag="P5316_00155"
     CDS             complement(38275..41580)
                     /gene="ileS"
                     /locus_tag="P5316_00155"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRR83390.1"
                     /translation="MFKEVPTQFDFPKAEAEVSRFWDEAGIYEKSLKQREGRPSFVFY
                     EGPPTANGLPHPGHCLTRIIKDVFPRYKTMTGHYCHRKAGWDTHGLPVEAEVCKGLGI
                     HTKQDIEAYGIEKFIHLCQQSVFRYTKEWEDLTRRLGFWINLEEAYVTYHQSYVESVW
                     WALKTLFDRGLLYQGHKIVWWWPQGGTALSSAEVGQGYRAVDDPSITVRFRIKGRPKT
                     SLLAWTTTPWTLPSNVALAVHPDVEYAHVSVEDEEFILAKALIPSVFKGLPVDVEKDL
                     RTIKVVKGSELVGIEYEPLFSYARPEGGKAYEVITADFVTLDTGTGIVHIAPAFGEDD
                     YRIGREKGLGFLQLIAPDGKFTPEATDFAGRFCKEADADIIRWLKDKGLLLKREQYRH
                     DYPFCWRAEDDPLIQYARKSWFIRTTDFRDAFLANNRKINWLPEHIRDGRFGDFLENN
                     VDWALSRERFWGTPLPIWECEKTGKQEAIASYAELTAKPGVSGMDVWEKAKAANPGLI
                     DDLKVHKPYIDAITYASPFAPGARMRRVPEVIDCWFDSGAMPFAQWGYPHKPGSVELF
                     KQRFPADFISEAIDQTRGWFYTLLAISTMLFGEKSSTQHSALSAQPYPHPFRNCIVLG
                     LVCGEDGKKLSKSKRNYKEPTYIFDREGADAMRWSMLSGQAPWTGARFKEESIATDQR
                     EFLIKLYNVYSFFVIYANIDKWSPVQSGLPARRFSACQSGRRNAGPTLSELDRWIMAE
                     LHQTIADVRASMDRYENYPACRRLVDFVDGLSNWYVRRSRDRFWRSGMDADKNAAYQT
                     LWTCLSELSKVIAPFVPFFAESLYQNLVRSQFADAPESVHLCDYPVPDEEAIDRSLLE
                     EMAVVREIASLGRSARMDAKIKVRQPLAVVEVVLADPSHRQWLEGHLPLIADELNVKR
                     IEFATNADQYVTYEVKPNFKSIGPKFGKLAPQIQKALATADAAALRRKLDETGKVSLD
                     VAGQTVELTPDDVQVALKAKSGWVAAQGRTAVVVLSTELTEELKAEGLARDVVHLIQT
                     ARKAENLDYQARIRLRINATGPLAEAIKAHDEYIRHETLAVDLRLDPSLVTAKHTGEI
                     EGMAVELEIETAG"
     gene            complement(41809..43002)
                     /locus_tag="P5316_00160"
     CDS             complement(41809..43002)
                     /locus_tag="P5316_00160"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR83391.1"
                     /translation="MLKEYSPFTPGVPVPLEFFIGRSQEIQTMIGSIRKSIAQKTIER
                     LFVTGERGIGKSSICTFVQSLAERDLRILPIHVFLGGVSTLEEMARRIFERLLRESAN
                     QPWFGKIKDFLGDHIKQMDIFGMSVEFSASGQILNRAVSDFIPTMKNLLNKFGEEREG
                     ILLILDDINGLASAPPFANWLKSLVDEFATTASAFPLTLVLVGLPERRRQLVNVQPSL
                     DRVFEIVEIRRFDQPETIDFFRRAFSKVNAEVDEDVLDLLARYSGGYPVFMHELGDAV
                     FKTDADNHIDLNDALEGIIRGAEVIGAKYVEPKVTAAIRSEKYQGILRKIAKEPFEHR
                     FTRKDAVARLTPTEAKVFDNFLRRMEELGAIRKDRDRGPGSYEFASEIYYLFFWMQAS
                     TPKER"
     gene            complement(43069..44571)
                     /locus_tag="P5316_00165"
     CDS             complement(43069..44571)
                     /locus_tag="P5316_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRR83392.1"
                     /translation="MPSDEAVPLVVCGRTGGDDPALLGRSWRFDTAVLLKLAVPNVAS
                     TVAQTLMSFTDFVIVKQMSAASEAQAAISSASLTFITLLAMPMGTMTCVTTMVSQSLG
                     AGRERDCAAYAWQGIWMALLYGMGALMLWPFVPAFYQMFGHEPTVTSMEVQYLHIRLL
                     SLGIAAATISLDNFFNGIHRPGINTVSAFLSVSVNVVLTYAWVLGKLGFPAMGVSGAA
                     WGTVVATVVRAVFQFSVMYFSSAASRFQPRKAWRWDREKMRRLCWVGWPAGAAFLTDI
                     AAWTVFQLAIIGRFGTADLAATGGVFRYLEVSFMPAVGIGMAVSTAVGKAIGQGHPTL
                     ARRITWIGTAFNAAYMSLMALLFVVYGEWLMRFISPDPAVVRLGTTLFIYAAVFQFFD
                     AIAITHSCALRGAGDTMWSSIVGAVEVWIILIGGGVLVARLRPDLGSVGPWAFAATFV
                     IAIGITLFARWRWGPWESFDVIGRNEPRGGFPVITAPIELPLSDPTNPPE"
     gene            45544..47109
                     /locus_tag="P5316_00170"
     CDS             45544..47109
                     /locus_tag="P5316_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRR83393.1"
                     /translation="MNKPLLAAAVCVMSGLASGEEPRIIPPFEVERPVVSANAIDDLV
                     WRALKKRGIKPANPCSDEVFVRRVYLDVIGTLPDPVEVNEFLQDRRPDKRAALIDTLL
                     SRDEFADYWSLKWCDLLRVKAEFPINLWPNAVQAYHRWIRDSIRENKPYDEYARELLT
                     SSGSNFRVPPVNFYRAVQGREPSAIAAAVALTFMGTRLDDWPEARRADMAVFFSRIAY
                     KKTGEWKEEIVCLDPAPTGPLEAVLPDDTRMRIPADQDPRMTFADWLIKPNNPWFARN
                     IVNRVWSWLLGRGIIHEPDDIRPDNPPVNPELLARLEKELVQARYDLKHVYRLILNSR
                     TYQQSSIPRSDRPEAEALFACYPVRRLDAEVLIDALCRLFESTESYMSPIPEPFTVIP
                     GYERTITLADGSITSQFLEMFGRPPRDTGLESERNNLPTEAQRLHLLNSSHIQKKIQN
                     SPRLRRLINNSRGNRPALIRVLYLNILSRYPTPAELAAAEEYFQTRGVKPDQAVNDLA
                     WALINTKEFLYRH"
     gene            47162..48514
                     /locus_tag="P5316_00175"
     CDS             47162..48514
                     /locus_tag="P5316_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRR83394.1"
                     /translation="MSGQYPEKMMTRRATLKQGLLGAAGLAFLDHEKAWAQPAAQSAH
                     STTRPAKARAVIQIWMWGGPSHLDTFDPKPDAGPDYCGPLNKPIATNVDGIRIGELLP
                     KLAQQADKYSIIRSMTHGVNAHETASYIVQTGRKPDRLVYPCVGAVVSVFKGYDGGYK
                     GLVPPYIVLTEPQGRFSEAGFLGQRYKPFATGGDPNQSRFVVEGIVAEGISDERQHSR
                     RQLLHKLDSLGRAMPANAQFAQLDRCEEKAYDLMFGEARKLFDLSSEKDEIRERYGRT
                     TFGQSCLMARRLVESGVPYVTINYKGWDTHKQHFETMRRKLPEMDQGMASLLQDLSDR
                     GLLDGTIVWWSGEFGRTPKVQWEAPWNGGRGHYGNCFSAVVAGGGFKGGRVVGASDAK
                     GQEVAARPVYPQDLIRSMYELLGIDPDGPLPNARGLAVPVLPAAEGDAQGGGPLKEIM
                     "
     gene            48532..50973
                     /locus_tag="P5316_00180"
     CDS             48532..50973
                     /locus_tag="P5316_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83395.1"
                     /translation="MTISRRLPLAAITLWAATAWGQSSQREPHIGYLYPAGGQQGKIV
                     LITVGGQNLKGVTDVYVSGSGVRGTVVQHFRPLNVIRQEQREALQKTMRELIQKRIAE
                     LPGGGYDFTALGREFGIARPDVVGPTAARRQDAPAPKNPPKKDRPSPQAKPDNQAKAP
                     ETQPAELPEHPLLYELEKKSLRELLHIRRELLNLRNRQLNAQIAESVLIRVAIEPNAK
                     PGDREIRLGTPLGLTNPMCFQVDQLAEITELEPNDPHAPSLLPDEPPAALPVIVNGQI
                     KPGDVDRFRFRAQRGQKLVIETWARHLVPYLADAVPGWFQATLALYDARGKEVAFADD
                     YRFNPDPVLMYEVPDDGVYTVEIRDSIYRGREDFVYRIAVAERPFITRVFPLGGQMGV
                     RTAASIDGWNLPAKQLFLATAGSTDGIRQTALRRNDNRSNEVSYAVDTLSESFETEPN
                     DTPEKARQIELPRIINGTISRPGDVDVFKFTGKAGTEIVAEVCARRLGSPLDSMLRMT
                     DALGRVLEWNDDHEDKESGLLTHHADSYLRTRLPEDDVYYVYLSDSQHHGGKPYAYRL
                     RLGAPRPDFALRLTPSSINVRAGGNAMVTVHAVRKDGFDGRIEVVLKDPPAGFALSGA
                     QIPSGRDQVRMTLTAPQIPPDKPISLQLEGRAEVAGRVLTRPVVPAEDMMQAFSYRHL
                     TPSQELMVATIGGRRGGPPIEVVNKGPVRIPSGGTAEVQIKTPAYRMQRELKFELREP
                     PRGVTLQDVTGAPQGLRLVFKADASAAQVGFADNLIVEVFTEAPARVPSSGGQKKSRA
                     FLGVLPAVPIEISPR"
     gene            51000..52178
                     /locus_tag="P5316_00185"
     CDS             51000..52178
                     /locus_tag="P5316_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRR83396.1"
                     /translation="MDFYAHHCGIPSVSVNISRFRGPSGVDEYHLVVTPTKYGSVNAQ
                     LEWVAHAYHKTLESIGLDARTAVLRRFFCSDLHNQAEALDACPISRCGNADDPCAISR
                     VGQPPGPPAKVILWAYHVRDRGNELEKSLRGAHLTLRRGDLSHHWTTGLTCTTGHGSY
                     GQTRGIFDRYNALLCEQGMTLADHVMRTWLFVRDIDANYQGLVAARREFFAEHGLTAD
                     THFIASSGIGGSSADPAAIVTLDAYAISGVRPEQIRFLAAPDHLSPTYVYGVTFERGT
                     SVAYRDRKHVIISGTASIDHQGKILYPGDVSRQLDRTLENIEALLDHAGATVNDMSAF
                     IVYIRDPSDHMIAQQQMRDRFGHAPIAVLVAPVCRPGWLIEVEGTAVVPETNPELLPF
                     "
     gene            complement(52336..53844)
                     /locus_tag="P5316_00190"
     CDS             complement(52336..53844)
                     /locus_tag="P5316_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRR83397.1"
                     /translation="MSDSAADDRHDEDSDLDSPLEAGFEEELDEALGEMSLESLIDLD
                     IPRGIPFAVPSAEGVKRGRVIAIHGDDIFVDMGGKSQGVLPATQYEDEPLPRVGDVVE
                     VTIEGYDRSEGILQLSRKGAVMATTWARLEEGHIVEGRVTGHNKGGLEMDINGVKAFM
                     PISQIELFRVEDTDLPGYVNRRLKCQVIEVRHEEKSIIVSRREVLRLEEEQSRERKYA
                     TLVEGQTVTGLVRQIMPYGAFVSLGDGVDGLLHVKDMSYSRVQDPKEVVREGQKLELM
                     VLKIDRDTRKISLGLKQVMPDPWLNIEVKYPVDSVITGRVTRLMDFGAFVELEPGVEG
                     LVPISEMTYERRIAHPKDVLQEGEVVRVRVIRLDTAAKRLALSIKQVGDDPWVGASVR
                     WPVNSVVSGTVKRIAAFGAFVELIPGVEGLVHISELSDQRVRSVGDVVKEGQSVQAKV
                     LEVDEESRRISLSIKQLASVPEYTGERTASTEPAAEPKKRKKPLKGGLDWKF"
     gene            complement(53980..56025)
                     /locus_tag="P5316_00195"
     CDS             complement(53980..56025)
                     /locus_tag="P5316_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRR83398.1"
                     /translation="MRTVSLSVLAAFVFSSVGPRAVLAQCRPYVFGMGHYLAKWAEEP
                     WSYDSQAMDKMVEIGATAVWVDFPWSSMEPVQGTIDWSYADHQVATAEAHGLQMFAFV
                     GTTPEWAKLYPHLPNYQTPPSEECLTAFQAFHSALAARYAGRVTYYQFWNEPSGCGWI
                     NENCLNGDDCSLFTLWQKRAYDALKAGNPDCVVSPGGFDGDPAGYVNCMYAQLAGQKA
                     FDAVSIHPYAPGGPGGPGTSGEGIDYSDLTSVRQVMVDHGDADKSIWITEYGWSTGNE
                     TQKADDLNEVLTELKKTDYSYVFFAKYLVLNDWNNHDENPNNDFCCFGLTDEYFNPKP
                     SFYTFKDFNKNFDDVVDFGADKTVGQPPMLVHFNDRSCVHGATAWFWEFGDGQTSTAQ
                     HPEHMYLYDGRFTVRLAVTGAAGPIALQKTDYIRVGTVAEIVNPSFEDAGNFLAGWSS
                     CLGGESSIKHNPSTHIPSPRFHDGSNSVGMSSDRAGDSLGPGAIWQTIAARPSHTYRV
                     RFWAAITASGGQYFDDFVELRVRDGDGAPLNCLNGGVNITNHSVLGAHLDGQAAPVWV
                     QLQAEITPRQDLLTVIVFWRFAGTVRAIHSLHLDDWAIEDITRRPGADFDGDLDVDDV
                     DYDLFQACATGPGVPISAADCDPADLDGDRDVDQSDFATFQRCFSGPGTVPASDCGD"
     gene            56565..58901
                     /locus_tag="P5316_00200"
     CDS             56565..58901
                     /locus_tag="P5316_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR83399.1"
                     /translation="MRNWWMHGLCPVQMRLFVIVVICISGVIAPAPAQETTAEPAPAA
                     AAEPDPRDATLESLFANYLHFALIGRFEVADQRFAQPLLSRPELNPMTPEAAETLIQL
                     SEKYENSMDTLLLLINKSTIGENAQKIMNLIREAHTRKRMDPARIKANIDLLKGDPVQ
                     RAVAVERLVESGEYAVPWMLGLLSDPDREDLLPFIARALPQLGRNAINPLLAALDCKH
                     ERTLQVVIESLGKIGYPQALPYLQNLATAKDTPASIQKTAAEAVAQILSKNQGIQLKP
                     AEELFRDLAEQHFAEIDSLRPDPREDRANLWRLRADARPGEDVVAPVAVDSRIYNLVR
                     CMECCQTSLSLKTDQPAVLALWVAANFRREARLGLDVESQETVDARKLDPSRPENFPR
                     SIYFARMAGPSCNQIVLARGIKQRDRNIALGAVTALNATAGRQAMTDVQAEGGTSMGE
                     ALYFPDLLVRVQAALALGKTLPPAPFHGSTEVVPVLASVLAPSEKKNYMLVDPEAESA
                     GVIQTGLVNSGAVLVLADRLSPALNQARREMTHLDGIFLASDMKQPTVTEAIEQLAKD
                     DRFCLTPIIVLVKENQTAVLDRIDGSDRRVGSIFVLTTPDKTTDPKIVEQLLAKLEQV
                     TIRCGLRPLSPESRLSLALQAAGVIKGMAQSESTVFNAQAALPAMVETLRQAGAEELR
                     AAVAAALAWMPAPQAQQAIAGIALAAEETESLRMTTFGLLADSARRFGNQLDDSLMSR
                     LMEQATSEPNLTLRTAASQAMGAMNPPVEKILPIITGK"
     gene            complement(59080..60039)
                     /locus_tag="P5316_00205"
     CDS             complement(59080..60039)
                     /locus_tag="P5316_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRR83400.1"
                     /translation="MAAAKTRKRRKKSEPAGPVGLKPSQVRAEPPSAVESLCKDIEKD
                     GGAVLTAYREPLGGRWVILAALPLEQVEPTPYQRGLSETHVKRLADVISRSGLYLDPV
                     IAVRVGPKRYQTPNGHHRTTAMKNQGAQSITALVVTEPQVARLILALNVEKAHNLREK
                     ASEVIRLARHLAEMPEGREEDYALEFEEAAFLTLGLCYEQNGRFAGGAYHPMLKRVDV
                     FLNQALPKSLARRAGYAEQLLKIDSRVTEIVGELKARGLESPYLRSFVVARINPIRFL
                     KGKTMPIEEALDKMEQAATKFKTDKINVSDLAKSGASAGPEED"
     gene            60897..61088
                     /locus_tag="P5316_00210"
     CDS             60897..61088
                     /locus_tag="P5316_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83401.1"
                     /translation="MRKASGLREAWLRIAAATLCIGTSYAGAVTIDFGPSFGAPSSDW
                     DNPDRSAFCLGSNVFDAEP"
     gene            61186..61512
                     /locus_tag="P5316_00215"
     CDS             61186..61512
                     /locus_tag="P5316_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83402.1"
                     /translation="MNFSSYVNLVAIRGFNGGGDTDTMILRAYNHANQLVAAKQITSV
                     FSTQGHTLTVTAPEIAYATVQAVAPLSGLFFDDLSFIFVPEPATALMTLLMGCFLCGH
                     WHRRRR"
     gene            complement(61624..>62383)
                     /locus_tag="P5316_00220"
     CDS             complement(61624..>62383)
                     /locus_tag="P5316_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83403.1"
                     /translation="KAARKAKGVIYQIGTQRRYSPPHLAGIELIHGGTLGKVMFMQGH
                     WHWAHRPKEPRKVARDGGMLVEQACHHTDVMSWVMRDKPPITCVAIARTEIPTPEGPN
                     VHNEQQSATIWQWPGGEIFSYTHLLWLARHFQTEQLHVHCQKGGVDLAHGVMHWADHD
                     PKTAAAPQEELKDQYADDPRGDWGMGTKEQLQAFVANIKADGKLPIHANVETGRIATL
                     MCLMGRMAAINAARNAYEPRVVHWKDLGSSTDPA"
BASE COUNT        11864 a        19349 c        19317 g        11853 t
ORIGIN      
        1 cgatccttga agtcctcagc ggccccagca ccacgcgcgg agttggggcg agtagatcag
       61 gagggcgacc acaagaccga cggcagtgcc tcccagcagt gttcccttga tgagcaagac
      121 tttcttgtcc agccatgccg ctgctccggg ccgcaccgcg tagtcgcgca actgctccat
      181 gcggttggcg atgctggaat gtcgccagct cttggcgtcg atcgggatgc cgttgagtgc
      241 cgcaatgcgg tataacgcat cggcaaacaa ctctgcggcg gacgcacaga tcgcctgagc
      301 agatgccggg ttgctgcgat ctgccgatat cgggggtggt cggtgaacaa aacagggcaa
      361 gtggcaaccc tctccgccag gggtcacatt cctggccccg aacaggtctg cctgaagctc
      421 aaattgtcgc gacaccgccc caaacccgag cagccagatc aggaggatca tcacggtggc
      481 cagcagcggc aggtaggcct ccaggtcggc gatctggtcg aagaacgtcg gccatttcga
      541 ggccgcaaac gcgatcagtt ccatgacgcc gccgacgatc aacatgctca gcacggcgaa
      601 cagcaggtag aactcgatat ggcggcattt gacgtgaccg gcctcgtgac cgaacaccgc
      661 ctcgatctta gcgtcgtcca tcatctccaa cagcccgtcc gacagcagaa tgtacctgac
      721 cggtttgatc aggcccatca ccgcggcgtt gaccaccatg ccgtcgcttt cccagatcag
      781 aatacggcgg tatctcagcc ccacgcgccg gcagaggcct tcgaggcgcc gtcgcagctc
      841 gccgtccggc agcacgcgcg tatgccagat gtaccgcagc atcaccgggg ccacgaggaa
      901 cacgagtcct gcgatcgcta ccagcactac ttggtcggcc cacacaatgc cgaacgcggc
      961 ctttcggatc ggggcggcat agcactgcac gaaatcgttg gccaccatga tcggtaacat
     1021 gggaacggca atgatgagca cgttctggcg gaacatgaat ctgacgaacg cccgcaggct
     1081 ccacggcggg cgggtgggga ccgatgccca gagtctcagt tccatcgcga cctgccggat
     1141 ggcgcggtcg gccggataga cggccacgta tgaagccaga atcgcggcga aaaacggagc
     1201 gatcgcgatg atttcgtcga gaccccagac ggcggcgatt ccgtcccaac ttcgaatgag
     1261 ccgaagccag tcggtcagga agaccagcga cccaaaactg agcaggagaa cagctcggat
     1321 gaccgtgtgg ccgtggccca ggcgtcgctg ggctgccggc aaccaggaag gttcacgttc
     1381 gagcttcgcc ctgacgcgac gcgtggtgat cgccccggcg agcccggcgg cgagaatgtg
     1441 gcccgcgact gcggcgaata tccaagcggg gctgcttacc agtacccatg cccgagccgg
     1501 tccctcgaag gggatgctca agacgatcgc gaaagccgaa aagacgataa actgcatgac
     1561 ttaccgagtc ccgtgcgaca tagcttgctt ccctttgccg cagtgggcaa gcagaggtca
     1621 tgcatcaagc gtgccgcgat tccttacggg ccgacggcct ccagccggtc gcccggcttg
     1681 acatgccggc cgttcacgaa atcctgccag ctcatgacgc gtttgccggc cggcttgagg
     1741 ccgtgaatct ccagcgttcc ctgccgcgtc gcgaccgtca gcagagaagt gatcgtcccc
     1801 ggcggctcgc tcgcttgagc gggaatggcc gaagccgtgg ctatgatgat ttcctccgtt
     1861 cgtccgtcgg cattgcggta caggcaccgg gcccccggcc aggaccacat cgcccgacac
     1921 cgcagagcga tttgctcggc cggctcatca aaccgcagca tgccgtccgc cttggtgagc
     1981 ttgggtgcct gcgtggccag cgactcgtcc tgcggctcgc cgggatggtg cagatcctgt
     2041 tccagcagct tcagcgttgc ccccagggcg tcgcaggcta tacctgccag acggtcgtgc
     2101 agctccgcgg ccgtctccgt ggacccgatc atggtctcgc gtcggatcag caccggaccg
     2161 gcatccaagc ggtcgacgag gcgaaagacg ctcacccccg ttttcggctc gcctttcaga
     2221 attgcccagt tgatcggcgc cgccccgcgg tacttcggca acagcgaact gtggatgttg
     2281 atacactcgc cggcgaacgc cgagcgaagt ggggcatgga gcttctggcc gaaagccgcg
     2341 acgatgccga ggtccgcgcc caggctcctg atctgttcaa cgaacccggc ctcgttcgtg
     2401 tcttcggtct tgtatacggg tagtccgagc gactccgcca tgccgccgac gggcgtggcg
     2461 gtttgtttct tgccgcggcc tgcgggccgg tcgggttgag tgaccaccgc ggcgatctcg
     2521 tgagggctgt gggccagcca tcgcagggcg ggcacagcga actcgccgga accgaaatag
     2581 accacgcgca tcaagccgct ccggtgcggg tcacaggccg ccccaatagc gcggcctctg
     2641 cccatatgtc cggatcataa gggtagaacc gggtacggcc aagtgatgcg gctcggccgc
     2701 tgaagcggtg cgaatctgcc ggccgagcgc caaccgactt gcggcggttt tgaccagtgg
     2761 accgcagtcg gtcggcggtg gcggggcttt ctgtattgct cccagctcgt caagggtcgt
     2821 cggcaggcat tccgttcgaa gactctcgtc gatgtcaacg ttcgtcactc tatttgcgac
     2881 aacccgcggc cggtcgcctg tggatgcaac cggcctgccg cgaatggcat catcgcggca
     2941 ttctgacgaa ctccacgtcg aattgttgcc ccatggtcag ttgatggtag acgcccgtga
     3001 ggcggtctgt tatgggatcg tatgcaagcg tatagtagtt gccgtcgtat ccacggtcct
     3061 tcagttcaac cacgagctga gtctcacctt tatcagaagc gacctcggcc ttggagacgt
     3121 tgaccggacc cgggttgaaa tatgcggcgt tcaccttccc tgcggtgtct acgcttcgga
     3181 tctccagcac gtagttgccg tccgtgcgca gccaccggcc gaccagcttg ccggtgtcaa
     3241 cggttttcgc gggtgccggc ctcgcgggcg ccttggtttc ctgcgcagcg gggggtgtgt
     3301 actgcggggc ggaaccgtct ttgttcaaaa catacgccgc catcgcgatc acgatcccga
     3361 ttgcgagcag cccacccacg aggggcaacc atacgggcag gccgctctgc gttctgcccg
     3421 cgggcttgtc ggctttctga tgttgcctga tctggcggcc catatatgcc tcaagcggag
     3481 caatgtggct gttcacatca tcgttctcat gatcgccgcg ttcagcgcgc cggtcaacga
     3541 agagcgagcc gcccggcgag cgtcacagaa tcgccgcgcc tggctcgaga agatgctttc
     3601 gtgttcgacc tcgtggtccg gagtcataca cagccctcca acaagcgggt ggaagggctg
     3661 ttcgggcatg gtccgggtcg tggtaggaaa ccgcccccct tgattcgcca agctaaatcc
     3721 aatctaatgg actatcggga ccgattgccg cgatccaccc ccgctgacag tgactccccg
     3781 cagagaggat gagcttcatg ggccacgata ccgagcatcg cgccgaccag ttgagcccca
     3841 tcagtcgccg ggcgttcttc cagagcttgg gcaacaaggc cttgaaactg ggcatcttcg
     3901 gaaccgccgc cgtatcgctc ggctcggacg acggcggttg ccgcatcaag atcggctatg
     3961 tcgacgcgaa ttgcggatac tgcgacggcg ccgggcagga ctacgaacac tgcgacggcg
     4021 attactgcga ttacgccaac tacggcgact actcggacga aggctgcggc tactgcgatg
     4081 gtaccggcca ggactacgag cattgcgacg gcgactactg cgactacgcc aactacgccg
     4141 acgcgtgacc gccaagccgg gtgtcttgtc agtcatggaa atcatcatca agccgaccga
     4201 agcctgcaac ggcacctgcg tctactgctc ggccgacggg acgttgggca agcgcaagat
     4261 cctcccgtgc gagaagctcg gcccgctgtt tgacatcttt gctaaatggg ttcgcaggga
     4321 cgaccgcaga aacctgcgtt tcctctggca cggcggcgag ccgatgttgt gcgggccgga
     4381 atactacgcc gagatcatgc aacagcagca acgcgcgttc ggcgacgacc ttgaccgcgt
     4441 ccgcaacgcc atgcaatcga acctctcgct ggtcgatgag cactgggttc ccgtgctgcg
     4501 ggacctactt aaggaccagg cgatcggcac ttcgttcgac atcgtcgacg gcgtccgcgg
     4561 cctggccggc ggcaagaacc tgcgcgagat ctgggtgcgg gcagtgcgcc tgcttcgaag
     4621 cgcgggcatc cgcgtcgggg ccgtatacgt ggcccaccgc caatcgctcg acagggtcgg
     4681 caacatctac aacttcttcc gcaacgccgg gttgcacgtc cgcgtgaacc ctctctacaa
     4741 ggagggccga gcggacagac gccaatcgca gccgttgtgg atcaccgccg acgagtacgg
     4801 ccagttcctc gtggacttgt gcgatctttg gcgtgccgac cggatgcgct ggtccatcat
     4861 gccgctgatg gaatggtacc gggcgtggaa agggcattcg cgtctctgct gcgacagccg
     4921 ggggatgtgc cacaccagcc acctgggtat caaccccgac ggagcagtct tcggctgcgg
     4981 gcgtcagtcg gaccatgggg ccaacaggct gggcaacatc ttcgaagacg atctcgacga
     5041 gatgctgcga cagcgcgagc aagggccgct ggccgggcga tcctccagtt tgcgtgacgg
     5101 cttctgccgc gattgccggt tttggaagtt ctgccacggc ggttgcccga tgatgggcct
     5161 gctctaccac ggcgacatga tgcgggagac gtatttctgc gccgcccgca agcgggtgtt
     5221 tgagcacttc gagaagcttt tcgggcctcc aactgtaagc gatcagccat cagctctcag
     5281 cggtcaacca ttagcccctc gacgcggctc gggaccagcg ttcagcggtc agcaatcagc
     5341 catcagcact cagcaagagg gcacgcagca agagggcgct cagcaagagg gcgctcacca
     5401 aaagaggccg gatctccccc ccttaccaag gggggggcgg ggggggtggg agtgccctac
     5461 ctcctccgga ggtgacggag cgtcttcact gaaccctgct tgccacgccg aagcgaagcg
     5521 aaggcgggaa ccccgcttgc ccggcgaagc cgtggcgaag ccgggaaccc tgaaccctct
     5581 atcccgcttc gtcgtgacca tcgaacggcc tgatggctgg ccgatgctcg acttcgtgcg
     5641 gatcgtcaca ccgcagctgg atctcgaacg cctggccctg ggacggctcg atccgcagga
     5701 acgcgcccgc gggcttgtca acctggagca tttcctcgcc tccccgagcg gctacaaacc
     5761 gtttgcccga cagttgacga tcatggccgg gcctgaattc gcttattcgc aaaacaaagc
     5821 agacgctatg caagacgggc ttctcggcac gcttcgccgc ctgcgtgccg agttgatgtt
     5881 ttcgtcggcg acgatcggtg acggcacgtt tgcccggcgg gcggccgatg cgagcgtgcc
     5941 ggtgcggctg gagttcggca ctgactacga cgaggccgtg ctgtccggcc tgctggacca
     6001 tttcctgcac actccaacct tgcaggtgcc gatcgagccg ttcttcaccc tggccggggc
     6061 aatcggtggg cagcgaagac tgacgctctg gcagatattc gacaatctga ccgggcgatg
     6121 cctgttcgtg gatgcccagc aacgcgtgtc cgtatcccgg tggtacgcac atcgaggcgc
     6181 gttcctcggc aaggtcggcg acagcatcaa ggttatgcgg gaatcggctg catggaagca
     6241 gatcgaggct tacgcggagc gagtcttcat cgagcagacg ccctgtgcgt tctgcgagca
     6301 ctatccgtac tgcggcggca tctggcgggc aacgagtgag gcagacacgg cctgcctcgt
     6361 ctggcggcgg ctgatggacc gtatggtcaa agcctatcgc ggttgcttgc cggccacatg
     6421 atccgcggat actaccgttg cacgcgggcc gagccgccct tggcgaaagc ttcgacttct
     6481 tccagccgtg ccatggtgac gtcgcccggg aaggtggtca gcagggcgcc gtgggcccag
     6541 ccgaggcgga gtgcctgctc gggttcgcgc ccggcgagca gaccgtagat cagcccggct
     6601 gcaaagccgt cgccgccgcc gatacggtcg aggacgtcga gctggcacgt ggggctcacg
     6661 aaccgcttgc cgtcgagcca cagcacggcc gcccagtcgt gccggttggt cgaatgcacc
     6721 tcgcgcagcg tcgtggcgac catcttgacg ttcgggaact gctcgaccac ccgctcgatc
     6781 atctggaaga acgtctcggg gtcgagcttc gacgcggcct tgtgggcgac ctccgggccg
     6841 gcgatgccca ggcccttctg caggtcctct tcgttgccga tcagggcgtc cacgttgccg
     6901 acgatcttgc gcagcgtctt ctgggctttt tccagccctc caatcggagc ccacagcttg
     6961 gcccggtagt tcagatcgaa ggagttgacc gccccggact ttcgcgcggc ctccatgccc
     7021 tcgatgatca gctcggaagt ggtctcggag agtgccgcga aaatgccgcc cgagtggaac
     7081 cagcgcaccc cgcgggcgaa gatgtcggcc cagtcgaaat cgccgggctt gagcagcgag
     7141 ccggcctcat tggaccggtt gtagaacacc accggcggcc ggacgccgta gccgcggtcg
     7201 ctgtacaccg tggcgatgtt cgggccgcgc acgccgtcat gctcgaacca tttgtagaac
     7261 ggtgtcacgc ccatttcccg aatgcggcct tggaccagct cgccaatgcc atagttaacc
     7321 atggcggtgg cgatgcccgt ccggagtttg aagcagtccg caaggttggc ggccacgttg
     7381 tattcgccgc ccgagacgtg gatgtccacc gaacgggcct tgcgaaacgg aaccaccccc
     7441 gggtccagcc ggtgaaccac cgccccaagc gccaggaagt ccagctcgca cgggtcttta
     7501 cgcacattca aaccgctcat cagtaccccc agaatcattc gagaaaagca tgcagatatc
     7561 cgccgcaaag cgcgatgtca accgcaccgg tctttcagga cggcgaccgt gaggagttga
     7621 ggcgatatcg cccggggagt gtgttgattg gcccccgcgc caaacggcgg cgcgtaatcc
     7681 acgtattgcg ggccggcggg atcaggctcg ccccgttcct ggtaggacga tgtgtaccag
     7741 tatcttccag agcagcctcc gacgtgtgct ctggccacat ttggtccgtt ggaagtacgg
     7801 attatctccg ttcagaccgt tgttcagatc agtcttgggc tcggttgtgg cttcatcgcc
     7861 ggtggcaccg tccgggttca gttggtagac atcgaatatg ctgagttgtc tgggggtgct
     7921 gtactcggcg gcctctgccg ccggcaggca acaggcggac agcatcagaa tgatcaatgc
     7981 gctgcccaca ccgaattgtg ggcccatcga tgacatgtgg tctttcccct gcacgtgatc
     8041 cgccgggcgc gaagcggcca ggcggtatga cacactactc gccgcggcac tcggccgtcg
     8101 ccggcacatc cgggccgctg acgcatccga cgaacacggc gaagtcgttc tggtcgatgt
     8161 cttcgtcacc atcgagaacc gcgttgaagc aggcggggtt cgtgaccgcc tgaccggagc
     8221 ccgtggcaca cgcctgaagg tgggcgaaat caacctgatc gacgtcggtg tctccgtcga
     8281 aatcgcaccc ccctccgccg ttgacggtga tcacggtttc atcgctcgcg gagagctccc
     8341 catcgttggc cgtcagtcgc agcgtataga cgcccgcctg cgagaaaccg gccgtcgtgt
     8401 ccaccgacgc agcgtcggca aacgtgacga cgcctggacc gtcaacctgg gtccagatgg
     8461 tggtcacgac tccaggcgga tcgggctggc cgtcatccga caccgtgccg tcaaggacag
     8521 caccctgaga caggtggact acctggtcgt cgcccgccga aacaaccggc ggctggttgg
     8581 gtccaggtgt cttcaccact cgaatcgcca cgtcgctggt ggtgcccgag ttgattgttc
     8641 gctgaccgtc tccggtgatc aaaccgaggt cggccgtgac gccggtgttg atgtccagcc
     8701 gggtcacgtc gaacacaccg gagacgccgg tcaggttcag cgtgacgctg ccaccccctt
     8761 gcagaaagat cgcatagtcg ttgccgaacc gcccgcactg cccccgactc acgagcccgg
     8821 aggcaggaga aaatgacaga aaagcaagat ccttgtagaa gctccagaag tagctcatgc
     8881 ggacggcacc cgggtcggtc gagacatcgt tgatgttgaa atcagggtag tgagcccgca
     8941 tcagactgcc catagtcgga tacgccatgg ccgccacaaa gttgcgatgg gtgttgcgga
     9001 tcccgaggac cacgtcgttg tcgtaggtcg ccggttcgta ccagtactcc tcgaaccaca
     9061 ccggcttgcc gtactggcgg tagttcaggg cgctctggta ctgcgtttcg gttcgcgcga
     9121 tctgcacctc gatatagtcg atgttggggc tgttggcgct gctggtgtcc acgctggtgt
     9181 ggtgactccc gatcggatga tcgtaggggt cctcgtccct gaccagttgc gcatcgtacg
     9241 ccacctgctg ctgccacgtg gaaccccagg gcgtgttgcg ctcgttcacc tcgccgtaca
     9301 ggcaccaccg gacgttatat gcggtgtagc gggccacgca gtatctgaac caccggctgt
     9361 tgcgctccag cgtgccgaat ttgacccacc agttgctgtt gtcggggaat ccgccgatgg
     9421 tcagtcccat catgatcccg cggtcgttgg cgtactgcac catctggtcc agtcgctgga
     9481 aataggccat attgggtgta tcagtctgcg gattcttgct tgggaatgca tcgggcacgc
     9541 ctgatcgggt gtagatggcc agccagatgc agccgaggaa ggtgttgaaa ttctgggcct
     9601 gccgggcgtt catccattgt tgccattcgg cggtggtgtt ttcctcggcg aaggaccact
     9661 gcgtgtcgcc gacggcaagg aagggcgtgc cgtcgctcag tttccaggcg tggccgtcgc
     9721 gatgaaggaa gccgcggtac agaacgttgg cggccaactc ctcgggggtc ggcccgatcg
     9781 cagtcagagt tccgctgaaa ccgttcatgc ccgcgtcggt cgatgaggtg gtccaggacc
     9841 agtcgccttg ggccgttggg gcgaaccgca ccctccagac gttgccccca tcccagaagc
     9901 ctttcatggt caacactttg ccgctggcgg ggccggaagt gccggtgaag gtggcggtca
     9961 ccgaaggtcc gttgaggtac ggattactgc ccggcgcgtc tgccgaaaac gccaattcgc
    10021 agacgcgaaa ggtggtggcc gttgaaggag gcgttccgat caggccggcc cgggctcctg
    10081 aaccggccaa caaccagaga gccacaccga gtgttacccg acccgagtat cgtctgcgtc
    10141 ccgatgaaac acagccattc atctcatgct ccctgttaag cgatagtcga gagccccgac
    10201 aagcgtcatg gttggaaagg tgataaggct ctttctcctc cggagaatgc tcacccgttg
    10261 aactgctgaa aaccatcggg tgccggtttc aatgtagcga aagcccgagg tgcgatcaag
    10321 aaaaaacgcc gtcactgaag gaggaacatc cacaaaccgc gagaacgatc gactgtcaga
    10381 cgcgccccgc cgacgcaatg agtgctgtga attggaaaga caccatctga ttttggggat
    10441 accagggatt cttgggacac catacctatc atccttgtct catctttcgg cttggttttt
    10501 gggatctgag tatgccgtcg agggtggcct caagtcgggc gagaaaggcc tgcttgccaa
    10561 tcgggcgtcc ggttcgttca tggcgttgat gcgactacag ctcgaccttg atgccggaac
    10621 aagaagtagg catatggggt atcccgatcg attcagggca agaaaaagga atggcgtccc
    10681 ccgatttgag gattgggggg tggcgagcaa gcaaatgggt ccgcgagcgc ggatcctacg
    10741 tcctcaggat aacagggcgg atcgccgacg agggcgtcgg cgctacggag aaggaccatg
    10801 ctggacgaat gcgctaccct cggcatccct gatgttccgc tttgcgacgg ggcattcggc
    10861 gggccggtgc gatcggcaga ttctgccggg taacaaggag gaagttgcgc attttgcggg
    10921 gttctttgac cgattgtcgg cagccgtaaa gtgcctaccc gcctcatccg ataagtccaa
    10981 tgggagccgt gcgcttgcag tgcgcgaggg gagccccgat gggggtgcca acatgggtca
    11041 gacccaacaa gatgatattg atgccctgct ggccggcgta agccaactgg ctgcggatgc
    11101 ggcagcggac atcgtgggcg acgacggcac cggtccgatt tcttcgggcc cggccacggc
    11161 cgtggcaaat gcgcccgcgg cggaagacgg cggcaaacgg gtccccaagc gaacgcagat
    11221 gccctcaggt atgccgaccg tggcacccgc gtcctcggag cggtcggaag atccccaacg
    11281 catactgcca ctcgaggtgc cggtgattgt cacccttgcc gagcgtacga tgccgctctc
    11341 gaagatactg gcgctcactc ccggttcgat catcgaattc gacaagcctt cggacgaact
    11401 gctcgacctg atgattaaca accggcgcat cggccgcgga caggcggtca aggtgggcga
    11461 aaacttcgga ctccgtgtca cgatgatcgg ctctattggc aatcgagtcg aggcgatgaa
    11521 cgcggtctga cctggccgtc acgctcggcg cccgccggtc cgcggaaatc ggcccgttct
    11581 cattcaacta ttgacgcaac cggtgccggc ttggatattg aggtcatgtc ggggacctat
    11641 tccgagtttt ccgtattccg cgggcacatc ggccagcgca ttcgctatta ccggcagcgt
    11701 ctcggcctga accagacgca gcttgcccgg cgtgccaatg tcaaccaggg ctttctcagt
    11761 gagatcgagc ggggcaagcg caagccgtcg ccctcgtcgc tgcgcgcgat cgcggaggcg
    11821 ctggacgtgg ctccggcggt gctgattgga gacgggctcg aacacgatac gcctcagccg
    11881 ctggaaaccc gagacctgcc tttgtttggc gcgataccgg ccggctcccc cgccgaaact
    11941 caggaacaga tcgagatgtt cccggtcctg cgacacctgt ggggacacga tcggtacatt
    12001 cttcgtttga cgttcgattc catggaaccg acactcaaac ccggcgacct cgtgctcgtg
    12061 gaataccggc aggacgtcaa tcccgagcac gtgcaaggtc gaatctgtgc ctgccttgtg
    12121 gatggcacgc cgacccttaa acgcgtctcc gttgagctgc aaaggagcag tcgaatcgtg
    12181 attctgcgcg gggacaatcc cacagttcct cccatgctga tcgacgagtc tcgggatttc
    12241 ctcattcagg ggattgtcac ccatctggtc agccgcgacc tatgagacct tgcgcgattc
    12301 attgcacgcg cgcgagtttg gttgcacatc cgccacggcc tcaggctacc gccgcccatt
    12361 agggcctcga agcacggtaa tgctttttaa tcgttgaaag accgtgcggc agcgtgcaaa
    12421 tcgtggcgca ttctattccc gctcgggctc gaatactcgc gacggccacg agaacaccgg
    12481 aatccccaaa tccattgccc gcaacgattt gcggaacaca ggcggctttg cactgatcgg
    12541 ctgccgagtt tggcacggca cgtgctactg ttcacgcgga ggattgtgga aaagcatgat
    12601 gaccggttct ggaacgaaga cgcgaagact gtgcagcttc aggctggccg atgtgttgtg
    12661 tcctgaaccg cgacaagtac tcgtgcactt gactccggag ctcgaggtca gcggtgaagt
    12721 ggtgttcatg agcgatcaag gtcagcaacc ggatcgcttc gcgatagtga atgtagaggg
    12781 gattctttcc ccgttgatcg tgccgacgga aaggctgcgg ggcgaagaac accctgaggc
    12841 gcgaggatat tgagactctg tcggccgcga ggcccgacag gaggaggagg aaaagcctgt
    12901 cgccttgatt caaggcgacg tccgtccgag gccttatcgg gaaaagcagg ccggagacgg
    12961 gcttgattct ctctcccagg cgagtggtta tcctcggatt tctctcgcct ggcgatagga
    13021 actatctgcg agccggttgg gatcaggaaa ccataccggg ttcgcggtcg attggtcgcc
    13081 ggactcctcc gcagtagcgg aagaggcaaa gaattggtta gccccccacc tgggtttggg
    13141 tggctccggt atagggttat gattctcccg aagaattgaa agcgtggctg gccacgccgt
    13201 cttcggaagg gatgctcagc ccggcggcag gcgtcggagc gggcaacaac gaatctctct
    13261 ctccctacca ggcttgggtt tcgtctcgaa gcccttgcct gggttttttt gggcgggcag
    13321 gcaaagccga gctgagcttt gagccggcgc acgacctgtt ttcgttcgct gatccccagg
    13381 ccaccgatcc agtctcatca agttagcaat ccataagtct tttacccaaa tgaacttatg
    13441 gatcagatgg tccgaagacc tgtcgcctga gccctgcatg agtgctaaca tacgtattgg
    13501 cagtatgttg cagtttttgg gggattcgga ttcggaggca tgtttgtcgc tagtaggcct
    13561 gtgaaagctg ccgatataaa agcgcttgat tgccgaaact cgttgctgta gactaacttg
    13621 cgtgccttgg ccggtggaag agcgatggac cggccagcac ttcggagcgg gattcccgtg
    13681 attgcgtaat cttcattgct gaaggcgagc accgggcgct tccagaggcg ggcagccggg
    13741 gccgtggaga gccgaggcgg atttaaagga cttgcgtcga ggtcagccat gcagaccatc
    13801 accaagaaag agttgatcga ccggatagcc gagagctgca accacaagcg cgtcgtggtc
    13861 aagcggatca tccagacatt ccttgaccag atcatcgacg aattgggtcg cggtaaccgg
    13921 ctggaatttc gggacttcgg cgtcttcgaa agcaaggtgc gcgccgccag aacggcccag
    13981 aatcccaaga ccctcgagcg ggtcgcggtt ccgtcgaagc gtacggtgaa gttcaaggta
    14041 gggcggctca tgaaagaccg gctggtggcg gccagcggcg attcgccttc gcctctcagc
    14101 ggcgtggctg gtcggaagcc cgcctcgggg aacgacggct ccaagacttg agccggcctc
    14161 gcgcacctgc ggcccatgac aacaccaagg acccctgagg aataacgttc aggggtcgtt
    14221 ttttgcgcgg cgttcctgcc ggattgcctg gcgtgccgag gagatcgtgc ggcaggtctt
    14281 gatccctgtg gcatcctcga cgctcaaccg tgtgtgcgca tcgaaacaga gcatgtccac
    14341 acggagtcgt ggacatgatg cccgcgcggc aggtcggcgt tgtaccgccc tgaaagcggg
    14401 ggtatcttgc ctttgtcatg ccggatgaaa gactgcgaca cggctggctg cgagcgttag
    14461 ggcgggcgga gatgccggcg cggatcgcgc tgcacgatgg ttactatact cacctgcgca
    14521 cctggaagca cgatttcttc gccgcaaccg gcctctatga ggggccgtcg ggccgggtgg
    14581 tgttgaagct tggtcgtacg gcaagtctgc tggtaattcc tggatcatgg atcggccgcc
    14641 ttctatgtga tcgcgagctg gacttgtatc gcgccgtcga cgacctgccg ggcgtgccca
    14701 agtgcctggg caaatggggc cggaccggct tggttcatgc ctttgtcgaa ggtcacccgc
    14761 tccagaagca ggaacgggtt gacgacgagt tctttcctcg tctggaacgg ctcatcaacg
    14821 agctgcatcg acgcaatatc gcttacgtgg acctcgaaaa acgagaaaac atcctggttg
    14881 gcgccgaggg acgtccgtgg ctgatcgact ttcagatttc ctggcgctgg ccttcggatg
    14941 ccgccgaacg gcgaggggga ctcaaacgtt tgattccgga ccggctgggt ctataccttc
    15001 tgaagcggtt gcaggaaggc gatcgctatc acctgctcaa gcatcgtcga cgccaccggc
    15061 ctgacacatt gacgcccgag cagatcgagg cgtcttatcg gctcggcttc tggcaccgga
    15121 tgcaccggcg ggtctcccgg ccgctgacgc tgttacgcag gtcggttctg aagatgctca
    15181 ccggccgatg gcaatcgctg aagcaggatg ggcccgagtt catggaacag ccggcagccg
    15241 gagcggccgg ccggatgtct gccgaaacga ggaacagtgg gacggcgtga catccgcatg
    15301 cgccggctga caggccgcca atggcacccg gcgggtgttg catgagatca tcgctgacgc
    15361 gacaggagct cgatcttatc gaggcgtttc gctcccgtgt cgagacgctt gcgggcacgc
    15421 ggcttggctt gccccagctt gagcggcatg accgtccgga tcactgggtc atggccagtc
    15481 gttggccgat ggaccgccac tggtggctcg aactggccgt ccgcccgacg gtcccgcaga
    15541 tccgcgtgac cgtggtgacc gacgacccga tgcgcagcct cgacgcggag gtcatgatct
    15601 cggaatccag cctcacgctt cgagagttca tcagtctggg tttccacgat gccggcctgg
    15661 cctggcccga gccgcccgtg gaacactatc gagaaaacgc tgagcggttc tgctttgcca
    15721 cgcctttgga cctcgcgtcg ttggacgagc tttgcggcga ggccatgcgt gaaaaggtgc
    15781 tgaagatgct ggacggatat catcggtgct ttaccggccg tgtcgtggga tcacgttgag
    15841 attgccgata gatggaccgg gcagtccccg ctgcggggtt agtgcaatgg ctgatgacgc
    15901 ggaacagatc atttctccag tcagcacgct gttggtcatc gacgacgacg tcctcgaccg
    15961 tctcggcagc acggtccgtc aactgtgcgt gggaatgatt gatgaggtcg tgcgggtgat
    16021 ggtactggcc cgctcgacct gccggatcga agaggcgata ggcccgtcgc agttgttcta
    16081 tctgcccagg cgttgctggc catggcactg gcggcggttg aaccccgatc aagccctggc
    16141 cctgatcggc gacgagaaac ccgatgtgat tcactgcatg tcggcggacc tgttgcacat
    16201 ggcgcaggat tgggtgatgg agtgggggag ccgagtcgtg gtgcacctga ccgacctttc
    16261 cgacgtccgc cgctttctca gattgcggct tccgccggaa ctggtcgtca tttgcacgac
    16321 gccgaccctt caacagtcgt tggttcgaaa gcggccggac ctggtcagcc gcatccgggt
    16381 gattcctctg ggcatccccg ccagcccgga gccggcgtgt tttgcggatc cggagcgggt
    16441 ccccactgtc ctggtaacgg ccccgttgac gcacgactgc ggtctgcacc tgattctcaa
    16501 ggcgatggat gccgtaatcg cagccgggca agacatgcat ctttttgtgt tgtcctcggg
    16561 tcccggcgag tacgtgtttc gcaaggtggt tcggcaactc gggttgcgtt cgcgggtaac
    16621 gtttgcaggc gagctgcacg actggacggc catgcaggcg gccatgcgcg acgcggactt
    16681 ctacattctg ccgggcgagc ggcggcgttt caccattagc acgctgatgg ccatggctac
    16741 aggcctggcg attatcgctc cgacggggac gatcggcgac tacttgatcg acggcgtgac
    16801 tgcctccctc ttcgaccctc agaaaccccg tcagatcagc gagaagctgt cggccctgct
    16861 ggcggacaag gccgttgccc ggaggcttgc ccaggggggg ttggagtaca ttcgggccca
    16921 ccatcaggcc agtgccatgg tttccagcat cgcgggtgtt tatcgagagc tttgcagccc
    16981 gggcgaatag ccggccgaac ccgcgccgcc cccggttata aacatcgtct ttgattcaac
    17041 ttgcggctac cgtcgggggg gcgtaacata tggcgcaagc atgtgactat acctccggag
    17101 ccttttgggc aggcacgcac ggaccgggat ctgcgccggt ggacggccaa ctcggaggtt
    17161 gaccgtcagg gacaaacgca aaatgccgcg gaggcaaacc ggcgtgggac gcgtccaaag
    17221 gtctggtgca gcttctgcgg gcggtgcgac gatgtccgcg acattaccct ttattccaga
    17281 cttgcgaata cttcgccctg gcgtacgttc gctcctgctc gtggcaatga cagcgctggc
    17341 ggcggtgccg ttccgagact cttcggccca ggagaccaac gccacacagc cgtctgcctc
    17401 ttcgctgact tcccaacagg caggccagat ctacgttctg ccgatacggg gcgagatcac
    17461 gaacgtcact catgatagtc tcaagcgacg actgggcatg actgagggga cgaacctcca
    17521 gattgtcgtt atcgagctcg ataccccagg cggtgccctg aaagccacgc ttgagatatg
    17581 ccacgagctc aaggcgttgc gagacgacgg tgtgcgtgtg gtcgcttggg ttaaccacga
    17641 ggcttacagc gccgggacga tcatcgccct cgcggctgac gagatcgtta tggcccggaa
    17701 cgcgaccatc ggcgactgcc agccgatcca gatcacccag gaaggagcct cggcgattcc
    17761 cgacgacatc aaagccaagg ccgtaagccc cttgctggcg gagctggagg actcggcaag
    17821 gcgaaacaac tacgaatacg aaaccctcct ggcactcatc gatcctgcgg tcagtttgta
    17881 ctggttgagg aacaccgaga ccggcgagag acggctggtg gacactaagg ggcgggacag
    17941 gctgttcggc ctggagcgaa aagagagcgc tggcggcggc gtcagcggcc tgctgaagat
    18001 cctgggcacg aacacggaga aagacacgaa ggactcaagc ggtgacgtgt cggattccag
    18061 gagccgcacg gcctggaagt acgtcgaaaa cgacccgagc ctggggcgaa tctcgcagcc
    18121 ggtcgatggg ccggatcaat tgttgaccat gcgaacggac aaggccaagg cctttggttt
    18181 ctgccgggcg acggtcagca acgacgcgga gcttcgcgaa tacctgaaag taaactggcc
    18241 ggtcacccgt ctggagagta actggatcga gtccatcatt aagtggctgg cttcgccgct
    18301 ggtgcggggc atcctgttcc tcttcatgct cttggcgatc tacacggaac tcaacactcc
    18361 gggtgtgggc atcgcgggtg ccgtcgccct gatttgcctg atcctgtttc ttggggcgcc
    18421 ctacctgacc ggctacacgg tgacctggga aattgtggca atcgtcctgg gcgcgatcct
    18481 gctgctgatc gagatattcg tcattcccgg tttcggagtg gccggtgtac tgggcgtgat
    18541 tctgatggcc gtcggtctgc tattctcctt cgccccatcc gagccgggct tcgaacgcga
    18601 gctgtggccg aggataccca ccttcggaat gatttatgtc tacctcaagc acgggttgta
    18661 cgccctgaca ggcggtctgg tgggctcgct ggtcggcatg tacttcatcg cccgttacct
    18721 gccgcgtgtg cccgtggccg gcagcttgat cgctccgaac cccgaccacg aggccgtcca
    18781 gctcgatgat ccttatcatg gggtcgccct ggttggtgac acgggaaccg ccgagaccct
    18841 gctgcggccg gcggggaagg cccgattcgg ccggatgctg gttgacgttg tctccgaagg
    18901 tgagtacatc gagagcggcg cggccgtcgt agttgtggag cggaggggca atcgcgtggt
    18961 agtgcgacgg gccggatgaa cgatatcctg aggcgactgc ctgtttgtgg accgctgcca
    19021 aagcgccttg cgggccggaa atacatacgc aggctgaact gatctctggc cccgaggtcg
    19081 cagcgtcgag ggtctgatgt atgcccgatt ggctgggtat tgttctgctg tatctgatcg
    19141 gtacggccgt cctggtggcg gagattttta tcccttcaca cggggtactg agcctggttg
    19201 cgttcgccat gctcggcttc gccgtctacg agacgtttca gatgagcggt ttgtggggcc
    19261 tggtcgggct gtcctcgctg gcagtcctga tccccgcgtc ggtgttgatg gtggtccgga
    19321 cgtggcaccg gactccgatc ggcaagcgca tctgcccccc gaacccgcaa ctgagcgagg
    19381 aagaccgaat gccgctggag ccgctgcggg ccgttatcgg gcggagaggt cgaaccgtga
    19441 ccttgctgcg gccggtggga acttgcgact ttcagggccg gcgatatgaa tgcaaagccg
    19501 agcagggcgt cattcagaag gatgtagagg tggaagccat tggactggtc gatcggacgc
    19561 tcgttgtccg gccggtgtcg agttgagctt tcggaaggca tgcgatatcg atctggacgg
    19621 tccagccgtg gtcaaggcgg tgcaacagat gacatccggc gtcggcacac tataatgagt
    19681 tggccaaagg gcgttgccgc ggttctcgaa agcgggcccc gacgatgaat aatctcagtt
    19741 cctcaaaaac aagaaagggc ttcacatgat agagcacatt ctcgcactga gcctgctggc
    19801 ccagaggacg ccgcagcacg acgggatccc gtggggcatc attgtcattg tcgcgatcgg
    19861 gctcctctgc ctcttcgtgc tagccctcgt gggacaggtg ttcaatctct ggctccaggc
    19921 gttcatgagc ggtgccggcg tgagaattac cgacctgatc ggcatgcggt ttcgtaaagt
    19981 cgacgcccgg acgatcgtga tgagcaagat ccgggcggtg aaggccggtt tgttggagat
    20041 cacgaccaac gacctggaga cgcactatct ggccggcgga cgggtacaga acgtcatttc
    20101 gtcgctgatc gccgcggatc gtgcccgcat cgatatgact ttcaagacgg cctgtgcgat
    20161 tgacctcgcc ggtcgtgatg tcttggaggc ggtgcacacc tcggttaatc cgaaggtgat
    20221 tgattgcccg aacccggcca gcggcaagca aagcatcgag gcggtcgcca aggacggcat
    20281 tcagctcaag gctcgggcgc gggtgacggt gcgaacgaac atcgcgcgct tggtcggcgg
    20341 tgccacggaa gaaacgatca ttgcccgtgt cggcgaaggc atcgtgacca gtatcggctc
    20401 ggccgagaac tacaaagtgg tgctcgaaaa cccggacgta atctccaaac gcgtactgga
    20461 aaagggtctg gacgcaggta cggcgttcga gattctctcg atcgacatcg ccgatgtgga
    20521 cgtcggggtc aacgtgggtg cccagcttca ggccgatcag gctgaagccg acaagcggcg
    20581 gttccaggcc gaggccgaga agcgggcggc gatggctcgc gcccgcgagg cggagatgcg
    20641 cgcattggtc gaagagaacc gcgccaaggt ggtcgaggcc gaggcgaccg taccgctggc
    20701 catcgccgag gccttccgca gcggcaacct gggcatcatg gattactacc ggatcaggaa
    20761 catccaggcc gacaccgcga tgcgcagcgc gatcggcggc gaaaccaagc cgccggagca
    20821 gaagaagtcg taagtgtgct gagcaagagg atctgagtga tgtggcagca cgtgacgatc
    20881 gcagaggcag gcagtagctg cttcttctat gtccttctgg tggtggtgct gatcgtcatg
    20941 ggctacctgc ggatgaagat cgagcaggtt ggaggcgagc agcgcaaggc aggtccgaag
    21001 cggccggtgc aacgcggcgt cccgcagagc cgcgtcgagg aacccggccg tcggttgccg
    21061 cccaggccac cggctcccgg acgcccgcta cagccgcaac gtcaggcgcc ccgcccaccg
    21121 gccgtctggg aggcgccggc acctcgccgc cctgatcaac ctcagccggt tgccagaccg
    21181 caaccgtccc ctcaacgacc gtcgtcacct accccgcgcc ccccgcaaga acgacgcgtt
    21241 gtcgccgctg cgataccgga ccaaggcgaa ccggcagcca aagagtttac ccggatcgct
    21301 tcgaaaccgt tgcccgtcgt ggcacccccg gaggcgaaga cgttggccgt tccggcggtc
    21361 ggggagtcag agctccaacc atcgaccagc gtcgaagcca ggcggccctt cgtgcgactt
    21421 gtgggcttgc ctctgataga gcttcagagg gcgtttgtct tcggcgagat actcgccccg
    21481 ccgctggcgc tgcgcgagga gcagaagctc ttctgacctg ttggatcagt ctccgagcgg
    21541 caggtcttgg tcgccggggc gacctcgaca ccgccctgcc taccgggcga tcagttccag
    21601 gccgatgggc ccgctgtgga gacctggaag cgtgagcttt attctgccgt cgcccagcgg
    21661 tccgagtgca ccctggcgtc ccagcacgta gcgtacgtgc cactggccgt tgatcttggg
    21721 tagatgatcc actttgatct gcacgtcgaa cggacccggc gtctccgatt tgttcgccag
    21781 gaataccaac gcgcgattgg gcattgtgta gttaccggcg tagaagcctt cgccgctgac
    21841 cagttcctgg gcataccacg gccgtgcctc gatcagtcgg tcgaaatcga tcgattccag
    21901 gagtcgccag agcgggcgaa tgcgtttcca ggtctcggcg gtgtcggcca cgtcgcccca
    21961 tgcgccgttg ccgctacaga cctgaatgtc gctcatgcag accgcggcca tcgcgatgtt
    22021 gtagggattc tgcgaggtct cgcgcatgcc gtacatcttg aagggggtac cggtcagcag
    22081 ggcgttgtag ctgttgcggt agtcctcggg cggagcctcc tggaatgact cgccgatcat
    22141 gcggcagtcg taaaagcccg tgatcagcat gtcgtggctg ccgccccatt gctccagacc
    22201 aaagcgggcc ttgtgccgct ggaggacttt tttgatccgg tagtagaacc ggtgctggat
    22261 ctcatcgagc gacagatccg catcctccgg gggaaaaggg aacaggcccg atttctgcga
    22321 agcaccctcc gggccgtccc ggcaaatcgg acctgtcccc ttttccggcc tcagccgccc
    22381 ccacgtcgcg ccacccaggc cgccgtcggc atagaagccg tcgaggccgt attcgcggca
    22441 ccagtagtcg gtcatccagg gcaggaactc ctgccagcgg ccgggagtgg cgtaaaaggt
    22501 gaagtcctgc aggtcccagt tcttctgata gtcgtcgagc gcatcatcaa accgcagcag
    22561 cgagctgcgc cccggcacga tcccgatgta accgatcgcg gccatgccga gacgatgcgt
    22621 ttctctcatc gcccgtttca tttcgcgatg gttgaccaga aagccctctc ggtcgcggaa
    22681 atcgttgctg atccaggtgt ggtgccagac catcgcctcc gtgccgttgg ccttgaatcg
    22741 tgccaagtcg acattgtcgg gatactcctg gaagctgggc atgcgacgcc agtgctggat
    22801 ggggccttcg gggtacttga tgtagtgaac catccgggtc gtgaagccga tgcgattgag
    22861 cggttgggcg ggtagccaaa gggtggcaaa tcgcagctta ctacccttga tcaccagggc
    22921 cacttcgcct ttcccgccgg cacggctgcc aagatcgatg tcgggggcca gcgacttgat
    22981 cagcacgccg gccttggcgt tgtgctcata gtacgcaccg cccttcatgc cggaggcgat
    23041 ggccactgcc attccggcct cggacgaggc cgcttgcttg gtccgagcgg caaactgcgg
    23101 cgccaagccg acgaatgcct ggatctgctt cggctcgacg ccctcgaccg tcaccgtcca
    23161 ccaccacacc gtcaaaaccg tgaatcgcgc cgtgacgtgt gccgtcgcct ttcgtccgct
    23221 gccgctgacc aggggcaggg ccatccgcaa agcgagcgga tcggcttgaa aggcaacttg
    23281 cccatcagga tcggcacgga atgcttcgcc gtcttcggag gtgacgatca cctcgggaaa
    23341 agcgccctcg atgagtgtat cgttgccgta aagcccggcc ttgaccactc cgtgcggcac
    23401 acccctgtca gcggacacca cggccgtggc gcctgtcggc ggccaagtcc actttacacc
    23461 gtcaggcgtc acctcgacct ggttggcctt gcggacgtcg ccctcgtact ccgcgtccag
    23521 acccttgcag aactcatgca tgtcgcgcag gcacgcctcg cggcgagctt tctcggcgct
    23581 ggagatcaac tcatactggc tgatgttgcg gactgccacg gtaccggtcg atccgaaccc
    23641 gtatttgccg ccggcagggt cggggtcttc gaactccaga agattgctcc aactcgacaa
    23701 cggcaccgcg acccgaaagc cgagccagat gtccggcgcc gcccgcaacg tgactcttgc
    23761 acctcgagct tccagcgaca gcagatgcca gccgcgcgcc agacgtctga actgaaactc
    23821 gccgatcagg caggcgttcg attcctccgg catgggcgac gagtgcgact gcccgccgat
    23881 catggaccgg ctgacggtga tcgacagggc ccgggcggtc cccttgagca ggtccaactc
    23941 gatcatcact tcgccaaagg cgtcagtcga acgcagcacg atgatgatcg gcttgtctcg
    24001 ttgctcgatc tcgaaataga caatctggtt gcgaagagga acccccccac aggctaccgt
    24061 gttcgtgatc tccgtccagc tatcgttcac acgaaccttc tgctcaggcg gacccttgaa
    24121 atccgcccgt tcgaatggca gaccgaggtt gtaccaggcc cgcttggcca cccattccat
    24181 cacgtgcagt tgccgggccc cgtcctcctg gccggcggcg aatccaagaa ccggctcggg
    24241 gatcgttgaa acgccatcct ctgccggagc gggcttgcgg aaccgttgcg ggccggcgca
    24301 gccggtcacc agaaccggac cgaggcaaaa caacagcacc gatgctagaa tgctctttga
    24361 gttcttcatg ggcggtagaa taatggctgc gtcgcgatgt cgcaactgac cttgacgagc
    24421 cggcgcgttt atcccgccgg tgggacacgt gcgcggaaga ccgcggggat aaaccccgcg
    24481 gctcttcaag gatcaccgtg ccgagaatcc tcttcggcac tcgccacaag gatgccgaga
    24541 aatgcccgca aagtcggact ctcaaaacct gccccccgac cagaagcatc agttgaccga
    24601 gtgggaccgc cgctgcgtct ggcatccgtt tacgcagcac tcgctgtgga acgtcgccga
    24661 gccgctgatc atcgtcggcg gtgagggcga atatctgctc gacgccgacg gcaatcgtta
    24721 catcgacggt cattccagcc tgtggtgcaa cgtccacgga caccgccgtc ccgagatcaa
    24781 cggcgccatc atcaagcagt tggatcgcat cgctcattcg acgcagcttg gcttggcctc
    24841 accgccggcc atccgtctgg caaagcgcct gatcgacatc gctccgccgg gcttgaccaa
    24901 ggtcttctat tccgacgacg gctccaccag cgtcgaagtc gccgcgaaaa tggcctacgg
    24961 ctactggcac cacaacggcc ggccgcagcg cagccgcttc atcgccctgc gaaacgccta
    25021 ccacggcgac accatcggag cggtcagcct cggcggcatc gacctgttcc atcgtgttta
    25081 ttcaccgatg ctgttcaaga ccgagtttgc cccctcgccc tattgctacc gctgccctct
    25141 gggcaagtgc cgggatacct gcaatctggc ctgcgccgac gagatcggtc gcttgctgag
    25201 cgagtatcgg gggcaggttg cggcggtggt ggttgagccg ctcattcagt gcgcagcggg
    25261 gatgatcact gctccccagg gtcacctgcg gcgggttcgc gagctgtgcg accggcatga
    25321 cgtgcttctg atcgccgatg aggtggcgac cggcatgggc cgcaccgggc ggatgttcgc
    25381 ttgtgagcac gagaacgtgg cgcccgacct gatgtgcgtt tccaaggggc ttacgggcgg
    25441 ctacctgccg ctggcagcga cgctggcgag cgaacggatc tacgaggcgt tccttgggcc
    25501 gatcgacagc ggccggacct tctaccacgg ccataccttc accggcaacc cgctcggttg
    25561 cgtggccgct ttggcgaacc tcgatatctt cgagtccgac cggacgctgg aacaactgcc
    25621 ccccaagatc gagcgactcg ccgagcatct ccggcggctg gccgacaacc cccatgtcgg
    25681 cgatgtgcgc caatgtggca tgatcgcggg cattgagctg gtcaaggaca agtccacccg
    25741 cgagcagtat ccctatggca tgcaagtcgg cgctcaggtg tgcgcccacg cccgccaata
    25801 cggagtcatc atccgtccgc tggctgatgt gattgtcatc atgcccccgc tggtcatctc
    25861 cgccgagaac atcgaccacc tgatgaatac cgttgagagg tgcatcacgg aggtcgtgcc
    25921 tcgtgttcaa acgggcgttg gcgacgggtt ggagtgaggg cgattggagt tctccctggt
    25981 caggtcgcag tggtcggttc aacccccgtc cactgacaac tgactactga aaactggcca
    26041 cttgcccatt ataatgccgc ccatggctct gaacttgacg ccggactact tcgaggcgga
    26101 tcgcaagtac cgccaggcgc ggacgccggc cgagaagctg gccgccctgg aggagatgct
    26161 ccgcaccatc cccaagcaca aggccagcga aaagaagcag gcagatctca aacgtcgcat
    26221 cagcgagctg cgtcagggtg agctgcagag agccagaaaa agcacggggg tcgatcctta
    26281 ccacatcgag cggcagggtg ccggccaggc gattctcatc ggtcctccca acagcggcaa
    26341 atcctcgatt gttgggaccc tgacgaaggc gccggttaag attaccgact ttccttacgg
    26401 cacgccgatc ccggtcccgg gtatggccca ctttgaagat gtgccgatcg aactggtgga
    26461 catgccgccg gtgacgcccg atcaccttcc gggcggcctc gtcaatgcca ttcgcaacgc
    26521 tgatgcggcg ctgatggtgg ttgatctgac ggccgaaagc gttctggaag atatcgacgg
    26581 cctgctggcc gcgctggccg ctaaggaaat cgagttcggc cggtcggagg ccgatggcga
    26641 ggaggagatg cgcgtcggtc cgcacggtat catcctgtgc aacaagatgg acatgcccgg
    26701 ggcggtcgat acgtttcaga tgctcaagga actccgtccg gccgagccgg agatgatgat
    26761 ggtttcaggc cgcacgggcg aaaaccttga tgtcctgatg cggaagctct tcggtctgct
    26821 ggacgtcatt cggatctact ccaaggagcc cggtcgaccc gtggaccgcg acaggccttt
    26881 cattctgccg gccggcagca ccgtggaaga gctcgccagg cacattcaca aggatctggc
    26941 cgccaacctg aaatatgccc gcgtctgggg cgagcatacc tacgacggcc agcaggtcca
    27001 cgggcgatac accctccacg accgggatat tgtcgaaatt cacgcgtgag agaacggaga
    27061 gcacccggat tccgcggcaa cgccgcttgt ttgcgcgtca atgggaatcc ggtcgatacg
    27121 aaccgggctg gtgttccaga tccgctgtgc gtactcattg accgagcggt cgctggagaa
    27181 cttgcccatg ttcgctacgt taagaatggc cttgcgggtc catgctgccg gatcccgcca
    27241 gagggcatcc acccgccgct gggcttccgc ataggactcg atgtcggcga gcaacatgta
    27301 gcgatcgcct tcggacgtga gccaatcgcg gacctgcttg aaaagatgcg gctgttcagg
    27361 attgaactcg ccgtcggcga cgccgtccag ggcctgtcgg accgcggggt tggagtggta
    27421 gacctcccaa gggttatacc ggggtcgaag ctcggccacc tcttcggccg tcagcccgaa
    27481 gaggaagaag ttctcggccc cgacggcctc gcgaatctca atgttggctc catccagcgt
    27541 accgatggtc agtgcgccgt tgagggcgaa cttcatgtta cccgtgcccg aggcctccat
    27601 acccgccgtc gagatctgct cggacaggtc ggctgccgga accatgatct ccgccacgga
    27661 cacacagtaa tcgggcacga agaccacgcg aagccggccg tcgagccgct tgtctgcgtt
    27721 gatcgtccgg gccacgtcgt ggatcagctt aatgatcagt ttggcacggt ggtaagaagg
    27781 cgccgccttg gccgcgaaga tgaaagcccg aggaacccgc gcttgcggtc tggacagcat
    27841 ctcgtgataa agcatgacca cgtgcaggat gttgagcagt tgacgcttgt actcgtgcaa
    27901 ccgtttggac tggacgtcga agagcgaatc gaccagcaag ttcacgcccg ccgtccgcaa
    27961 gacgtgctcg cagagcctgc gtttgtttgc ctgtttgact tgccggaact cgtcctggaa
    28021 ttcgggatcg tcggcatacc gtgtgagtcc cttgagctga ttgagatcac agacccagtc
    28081 ctcgcccaga cgccgggtga tggccgcggc gagaccggga ttggccgctt tcagccaccg
    28141 gcgcggggtg attccattgg tcacgttcgt gaaacggtca ggccacagct cggcgaaatc
    28201 cggcacgagc gtctggcgga taagacggct gtgcagctcg gccacgccat tgacggtatg
    28261 gctgccgacg atagccaaat gtgccatgcg gatggaacgc gggttatcct cctggatcag
    28321 cgacatgcgt cgcttgcggc cgtcgtcacc cggccagcgc cgatcaacct gctccagaaa
    28381 ccgtttgttg atctcgtaga tgatctggag gtggcggggc agccactgtt cgaacatcca
    28441 caccggccag gtttcgaggg cctcgggtag gagcgtatgg ttggtatagg cgaagatggc
    28501 ctgggtcatc tcccacgccc ggtcccaatc gaggccccga tcatcgacca gaatgcgcat
    28561 catctccgca atcgcgatcg acgggtgagt atcattcagg tggatcgcca ccttgttggg
    28621 tagatcatcg atggtatcgt ggtcgcggtc gtatcgccgc agaatgtcgg ccagcgtaca
    28681 ggccacgaag aagtactgct ggaccagtcg caggtgccgg cctctctgtg tctggtcggc
    28741 cgggtagagt accttcgaga tcgtttcgtt catgactttt tcgcgaaccg cctcgagata
    28801 cccgccttga ttgaaggctt cgaggttgag catttcggac gcgcttgagg cccacagtcg
    28861 caggatgttc acggctcgcc cgccatagcc gaccatggga atgtcgtacg gtatgccgac
    28921 gacggtgtgg tatcccaccc acgccggccg atatcgcccc tgcgaatccg tgcggtgctc
    28981 gacgtgcccc cggagtcgca cgatggtcgc gaactcgggc cgcatcagtt cccacggcgt
    29041 gccgaagcgc aaccagtact ccggtctttc aacttgccag ccgttctcga tctcctggtg
    29101 gaacatcccg tgttcgtagc gaatgctgta gccatacacc ggataaccct gagacgcggc
    29161 cgaatccatg tagcaggcgg ccaggcgccc cagaccaccg ttgcccaggc cggcgtccgg
    29221 ctcaacgtcg aagatgtgat cgaaatcgct gtcttcggaa acgacgctgc agcactcacc
    29281 atacaggccc agattgacca gattgttgcg caggagccga ccgagcagga actcgagcga
    29341 caggtagtac actcgtttca cgtcctgctt ggcgtatgcg gcctgcgtct ggatcatccg
    29401 ctcgacggcc aagtcccgga ccgccagcgc gagactgacg tacttgtcgt ggggtgtcgc
    29461 cccttcccag tgcttgcctt ggctcagtct gaggtgctgc aaggcccggt cgatcagctt
    29521 gctacgaccg gcgattcgct cttgaccctg ttgaatggcc gctgcgactt tgatttggag
    29581 tttccgcgtg gacacctgct gccccataat gagctcctga tccgacgttc cgggttctca
    29641 agacagaggc ctgcctcggc tcctgaggct gccgccaggg taggcttgac tgagttgtct
    29701 gaaacctaag tctacatgat gtcgtcatcc ctggaaagat gccggaaacc gcgcgctccg
    29761 gcgtgacgga tcatcgccag gacagaaaac gaccgaaacg catccgtatt gcccgacaag
    29821 acaattgtaa cggctatgcc cgcggcgtat attgagcggc tgactgaacc ggcgcggatc
    29881 agtgagtgga ggaactggca gggatgccaa aaaactggac aatcatcccg gcatggccag
    29941 agtgtgccgc ggctgcgagg cgactgggca ttcatcccct gctggcccag gtgctgcaca
    30001 accgcggcgt ggccgatccc gatgcggcac gactttttct caacccggaa ttgaagtgcc
    30061 tgctggcgcc ggagactctt cccggggcag tcgaagctgc cactatcatc gcccgcaaga
    30121 ttcgcgagcg gcgacccatc gtcatttacg gcgactatga cgtcgacggc atcacggcaa
    30181 cggccattct ctggcacctg ctgacgctgg ccggggctcg ggtctgtttc tacgtgccca
    30241 atcggctgga ggaaggatat ggggtcaacg ccgaggcgct gcgacagatc cgacaagacg
    30301 gggccgatac ggtgatcacc gtggactgtg gtatcactgc ggtgaaagaa gcagagattg
    30361 cccgtgagat cggcctgacg ctgatcatta ccgaccatca ttcgcccggc gcgacgctgc
    30421 ccgaggccga cgccatcgtc cacccaaccg ccgccggggc ttacgcgaac ccggacatct
    30481 gcggggccgg agtggcgttc aagctggcct gggcggtggc acgcatgctt tcccaatcgg
    30541 agcgggttaa gcccgagttc cgcaagtttc tcatcgacgc gaccagtctt gcggcgctgg
    30601 gaactatcgc cgacgtcgtg ccgctgagcg gcgagaaccg cattctggcc cgattcggtc
    30661 tgctgggtgt gaacgatacg cggctcatcg gcctgcgggc cctgattgaa tcagcccggc
    30721 tctcggacca gaagatcgac agcgaacacg tgggcttctg gctggcaccg cgactcaatg
    30781 ccgcggggcg catgggccac gctcgaatgg ccgtcgagct gctgaccctg gctgacgccc
    30841 cgcgtgcccg agagattgcc gcctacctgg aaagccagaa ccgcgggcgg caggcagtcg
    30901 aacgacagat cctcaaggaa gcgtgcggga tgattgacgc cggcaacctg gccggagacg
    30961 cccgccgggc gatcgtcctg gcctgcgaaa agtggcacgc gggcgtgatc ggcatcgtcg
    31021 cctcgcgcat cgtggatcga tattgccgcc cgacggtgtt gatctcgctg gaaaacggtc
    31081 agggacaggg ctcggcccgc agcatccgaa acttcaagat gcaccaggcg ttggccgagt
    31141 gctcggatca cctggaggcg ttcggcggac acgccatggc cgccggcctg cgcattcggc
    31201 atgaaaacat cgaatcattc accgaaagat ttgttgagct ggccaaccgg acattgacgt
    31261 gcaaggatct cgaaccctcg ctgaggctgg atgccgagat cgggctgaac gacctgccgg
    31321 atgctctggt ccacgagttg cagaggctcc agccttttgg cgtgggcaac cccaaaccga
    31381 agttcgcatc gggcatcctg caactcgcgg gcgagccgcg gctggtgggc aagaacggtc
    31441 agcacttgca gttcgcactg acggatggtc ggatcacgcg caaggccatt gcgttcaacc
    31501 agaaggatgc gctccggcct ttgctcgacc accgtcgttg cagggtggcc ttcgaaccta
    31561 ttctcgacac gtacaacggg cgaacgtcgg tcaagctgca agtcattgac atggcatttc
    31621 cggaatgacg cggcgaaggc aggtctccgc gtgagcacgc tgctgatctt gagtcgcaga
    31681 ccggagagtc cgatttgaaa tctcaaatct cacatctcag attgcgcagt cctgggcctg
    31741 ggcaagacga aggatgaaag cggggcagac aacccccaca tgacggtctc ttgtttcgtt
    31801 tcatccctat ccctcattct tcatcctttt ctgaaccctc aactcatgcc ttgaagtatc
    31861 gcgcctcggg gtgatgggcg atgatcgccg aaacggactg ttcaggaacc atctgcaatt
    31921 gttcggtcag ccggacgccg atgcgctccg ggtggagaat ccgccagagt ttgacttggt
    31981 cctcgaggtt cgggcacgcg gggtatccga agctgtagcg actcccctgg tagccttgtt
    32041 gaaggagctt gcgcaactca ggggcatccc gaccggcgat gcccagttca acccggactc
    32101 gcttgtgggt ccattcggcc agtgattcgg ccccttcgac gctcaggccg tgcaagtaga
    32161 gatagtcggt gtatcgcccc gcttcgaacc attcgcgagc gacctcgctg gcgtgcgggc
    32221 ccatggtcac gaccgacatg ccgatcacgt cgtattgccc cgttgacttc ggccgccaga
    32281 aatcggagag acaccaccag gggggcttct gctggcgcgg gaaatcgaac cggaccaact
    32341 cgcgatctcg atcttgcggg tcgtagatga tcaacgagtt gccctcgctc tggcagggcc
    32401 agtagccgta gatcaccttg ggttgaagga tgttctcggc gttgcagcga gccaccaggt
    32461 cgcgatagat cggccgcacg gtttcgtcga gaaagcggtc gaattcctcc tgcggacggc
    32521 cgcgcttctt gaattgccac tggctctgga aaagcatgaa ttcgttgatg aacgacaagg
    32581 cctcggtcag cgggatgttc tcgatgacct gcgcccccca gaacggcggg gtggggatgg
    32641 aaacatcggt cgcaatgccg ctccgctcga tgcgatcgtc tcggaggacc gccgggcgcg
    32701 gagtcgatga gcgggcacag cccgacccca tttcgtactc gccgggatcg gaacctcccg
    32761 ccgtctgtac gggaatcgat tgcaccccct gctcgcagat tcgccgcatc aggcccaacc
    32821 cctcgaacgc gtccttggca tagaacaacg agcctcgata aaggggacgc agctctcttt
    32881 cgacgtagga ccgggtcagc gccgccccgc cgagaatcac cgtcggcgtc agcccgcgtt
    32941 cgttaagcac cagcaggttg tcgcgcatca caagagtgct cttgaccagc aggccggaca
    33001 tgccgatgac ctgggccttg tgctcctgcc aggcgtcgat catcgctttg atcggcacct
    33061 tgatgcccag attgtgaacc ttgaagccgt tgttggtcag cagaatgtcc accagattct
    33121 tgccgatgtc gtggacgtcg cctttaaccg tcgccaacac gatcgagccc ctgccggtcg
    33181 catcggtacg ttccatgaac ggctcgagat gagcaacggc ggtcttcatc gtctcggccg
    33241 actgaagcac gaacggaagc tgcatctgcc cctcgccgaa cagctcgccg acagccttca
    33301 tgcccggtag tagcagctca ttgatgatct gcagaggttt gtaggttttc atcgcctcat
    33361 cgagatcatc cgtcagaccg gtgcggtcgc cgtcgatgat ccgctgttta agccgctcct
    33421 cgatggtggc tggcgggcgt gcggcggctt cgggccgact gacggcatcg tcgccgaact
    33481 gagccagaag atcggccaga gggtcatacc cctcctggcg atcgtcgaag atcagtctgc
    33541 gggcgatctc gcgaagcttc gggtcgatct tgtagagcgg ctggaagccg ccggcgtgga
    33601 cgatggccgc gtcgagcccc cgctcacgag catagtgcag gaaaacactg ttcagcacct
    33661 gccgagccgc cggcttgatg ccgaagctga tattgctgac gcccagaacc gtgtgacaat
    33721 cgggcagttc ctgcttgatg cggcgaaggg cttcaagcgt ttcgaggccg agtcggcggt
    33781 cctcggcgtt gccggtacaa atagtgaagg tcagcggatc gaacaggagg tcgctggacc
    33841 gcagcccgta cttaccggtc gccagatcgt gcaggcggcg cgcaatcccg accttgcggt
    33901 ccgccgtctt ggccatgcct tcctcgtcga tggccaacgc gaccaccgcc gccccatact
    33961 tccggcaaat gggcagaatc tcggcgcagc gcttctcgcc gtcttcaagg ttgacgctat
    34021 tgacgataca tcggccgcca accagtctca gggccgcctc gatgaccgaa gcctcggtcg
    34081 agtcgatcat tagtggcacg ggcacctcgg tggccaagcg ggcaatcaac cgggtcatat
    34141 ccgccatctc gtcgcggccg acgtaggcgg tgcacacatc gatcatatga ctgccggccc
    34201 gaacctggtc cttggccatc tctaccatgc cgtcgaagtc ctcggcggcc agcatttcac
    34261 ggaacttctt cgagccgttg gcgttggttc gctcgccgat gatcagaaaa ctgttgtctt
    34321 ggcgaatggt gatgctctgg tagagactgg agacgctggg ctcaaatcgg ggactccgcg
    34381 gcgcaggcct gcgccctttc atagccttga ccacctgagc catgtgctcg ggcgtcgtcc
    34441 cgcagcagcc gccgaccatg ttgaccccat cctgctcgac gaattcaacc agccggcggg
    34501 tgaggtcctc gggcgacagg gggtatctcg gcttcccatc caccaactcg ggcaggccgg
    34561 cgttgggcac gacgctgatc cggcgcgtgc agtgttcgct gagatagcgg atatgttcgc
    34621 tcatctgttc ggggccggtt gcgcagttga ggccgatgac ttcgatctgc ggataggcat
    34681 cgagcaccgt cagggcggcg ccgatgtcac agccgaccag catggtgccg gtggtttcca
    34741 tggtgacctg gaccatgacc ggcacgcgac ggccgcattc ggcgaagact atctccgcgg
    34801 ccgccagtcc ggccttggcc tgcaggatgt cctgacacgt ttcgaaaagg agtgcgtcga
    34861 cgccctcgtc catcagcccg cggatctgct cggcgtagct gtcctcgagc tcgtcgaagg
    34921 tgacttgtcg aagcgagggc agtttcgtgc ccgggccgat cgagccgatg acgaatcgcg
    34981 gatgatcggg ggcgctgaag cggcggcagg cctcacgggc gatccggacg gccgcgcggt
    35041 tcaattcgcg agtctgcccg gcgagtccga actcggccag aacgatcttg ttggctccga
    35101 aggtgttggt ctcaacggcg tcgcagccga ctccgaggaa tcgcgcgtgg atctcgcata
    35161 ctacgtccgg acgcgtcagg cagagaatct cggtgcagtt ttccaagccg cggtagtcag
    35221 acaacggcag gtcgaacgca tggacgctgg ttcccatcgc cccgtcgagg accatgatgc
    35281 gttcatcgag tgcccgtaag aaagccgaat ccatactcgc tccgtcaggc cgatcgccaa
    35341 ctggggatcg tagtcctcgt ggcctcggtc ggcaatcggc cggcaaccac gtgattatcg
    35401 accaaaacgc ggataggata ccggcccata gaaggaattc gaccagtggg tgtgcgtacc
    35461 atatccctga cggaggattg ggatcttacc ggtcccgatg ggctcgaagc ccttggcaca
    35521 ccgggtcggc agagaagcag aggagaagtg ccgtggccaa gacatggaaa gccggtgtta
    35581 tcggttgtgg atcggttgct caacatcttc atctgcccgg gtacatgcgg acgccgggcg
    35641 tcgagctggt cgctgcctgc gacccggagc cggcgcgaca caaggaggtg cgacaaatca
    35701 agaagaacct gcacctgtac cgcgattacc gggagatgct cgcccgtgag gaactggacg
    35761 tgatctcggt ggccagcccg aacagatttc atgccgagca tgccgttgcg gccctcgaac
    35821 acggcgccca cgtcctgctg gaaaagcctg cggccctgag catgagggaa atcgcggcca
    35881 tcagaaaggc cttggccaag agcggccgac agttgatcgt gggcttttcg cagcggttca
    35941 gccgcggcaa ccggaagctc aatgccctgg ttcgcggcgg ggtcatcggc gagccttaca
    36001 tgatccggat ccgctttgcc cactctggcc cgtaccccgg ctgggccaag gacccctggt
    36061 tttatagtcc cgtcaaggcc ggcggcgggg ctctgctgga catgggcatc catgcgatcg
    36121 accaggctca atggcacatc ggaccggtga gaagcgtcca ggcccacacc gccacgctgc
    36181 gcaagaaaat caaggtggag gacaacgcgg tgctgctgct cgaattcgcc ggcggcaaag
    36241 ccctgggtta catcgaggta gggtggacca gcccgtcggg cttcaacggc atcgaggtga
    36301 tgggcgacga aggctgcatt atcaccgact acaccggcgt catgcgcttg acgacgggaa
    36361 aggtgacgcc ggacaccaag gcccggctca agctgaagac ccgagtgatc gacaaggcgc
    36421 ccaccaccgg cggctggtcg gttgaaatcc aggaagtggt caaggctttt cggcgaaaca
    36481 gcaacctcgg cagcggcatc gacgccggcg gctcggccct ggcagtggcc ctcgccgcct
    36541 acgagtcctc gcggaccgga aagcgcgcca aggtagcaag catcaggtag ccttccccct
    36601 caaccaggcc gcccttcccc ccttggcaag gggggacaaa ggggggttct gctcgaccga
    36661 gaatcccttc ttggtcgcac cttggcgcaa tccctcaccg cgagtgaggg cgaaagaggc
    36721 tcggcccagg cacgtcctga gcctgaaccg accgaatcat cgaatctcaa cccgatcgcc
    36781 gaccagcctg aggcgtcctt cggcatacaa ccggatcgcc tccggatatg ccaggatttc
    36841 ctgctcgaaa acgcgagcag caagcgtatc ggcatcatcg ccgggcagga cggcgacctt
    36901 gcgctgcagg ataatcggcc cggcatcgta ttcgttgttg gcgaagtgga cggtgcagcc
    36961 actctccttg tcgccgctgg ccagcaccgc ctcgtggacg tgatgcccgt agaagccttt
    37021 gccgccgtgc ttgggcagca gggccgggtg gatgttcatc acccgcccga gccagcggtc
    37081 tgggatccgc cagtagcagg tccagcctgc ctggcaagct aactgcacgt catgagcttc
    37141 cagggcttcg acgaccagtc ggctgaactc ggcctcgtcg gggtgctctt tctttcggat
    37201 gacaaccacc ggcaggccgg cggcccgtgc ccgctcgacg cccttgacgt ccggcagcga
    37261 cgaaatcacc accgcgacct cggccggcag ttgtcctgcc ttgatcagct cgatgaagtt
    37321 ctgcagcgtc cgcccgctgc cggagatcag aacgccgagt ctcagtttct ggtttgcgtt
    37381 gcccatatca tcgtaccctt ggtattccct gacccgacag agggcactga aacatcgtta
    37441 accacgtgct tgtcgcgagt acgcattctc ccaccggcag tcatcgtact cgcctctcga
    37501 caccatcacc actgcatcgt cggcgtagtc gccgttcatc caattgtgcc ctgccttgcc
    37561 ggaggttgcc agatgctcga tcttctctac aacctcgagc cacccatctt ccgaatactg
    37621 ccaatcgaag caaagtagtc cacccgcgtt ttcccgcaag cgcaaaacca ggtcttgccc
    37681 gacggggtgc gctgtcagtt gcaggcctcc ttgcgcggta acaaatggca gagcgtgtat
    37741 ggggatcggt tgactcgctg ggtcttccag gacgcgcaac ctcttcgcca ggaccagcag
    37801 gtcggccgcc tctccggtga tgaggatggt gtcctcgccc aggaactcac gaaagtaggc
    37861 catccgaagc atgctgctga gccccgctct tgttcgcaat cccaatcaac tgccggccac
    37921 gaagcatgga gtcaacaggc acgatcaaat cggtgtcggc gccgatcatg atcggccctg
    37981 gcgaatcgcc ctctgcaaaa aggcatcagt tagcggcttg ccccgcgtac ctgccacttc
    38041 ctgatttctt ccagcgtggg ccgtttcgtt tcatctcgca gggccgattt cagaatggca
    38101 gtggcctcct ggttcagact ccggcgggtg cgggccgcac ggcgcttaag cctggtgtgg
    38161 acctcgggcg ggacgttctt gagcaacaat ccagccatga cctgagtctc cgataccgtt
    38221 ttggtatcac cattgtatcg ctcgggagaa agcggaacac cgggtcccgc agacctaccc
    38281 tgccgtctcg atctcgagct ccaccgccat gccctcgatt tcgcccgtgt gtttggctgt
    38341 tacgagcgac ggatcgagcc tcagatcaac cgccagcgtc tcatggcgga tgtattcgtc
    38401 atgagccttg atcgcctcgg ccagcgggcc ggtcgcattg atccgcagcc ggatgcgagc
    38461 ctggtagtcc aggttctcgg ccttgcgggc ggtctggatc aggtggacca cgtcgcgggc
    38521 taggccctcg gccttgagtt cctctgtcag ctcggtgctg agtactacca ccgccgtgcg
    38581 gccctgggcg gccacccatc ccgacttggc cttgagagcc acctgcacat catccggagt
    38641 cagctccacc gtctgtccgg cgacatccag cgagaccttg cccgtttcat cgagcttgcg
    38701 gcgcaaggcg gccgcatccg ccgtggccag cgccttctga atctgcggag ccagcttgcc
    38761 aaacttgggg ccgatggact tgaaattcgg tttgacttcg taggtgacgt actggtcggc
    38821 gttcgtggcg aactcgatcc gcttcacgtt cagttcatcg gcgatcaacg gtagatgccc
    38881 ttcgagccac tgccggtgcg agggatcggc cagcaccacc tcgaccacgg ccagcggctg
    38941 gcgaaccttg atcttcgcgt ccatgcgggc cgatcggccg agcgaggcga tctcgcggac
    39001 aaccgccatc tcttccagca gcgacctgtc gatcgcctcc tcatcgggca ccgggtaatc
    39061 gcacaggtgt acgctttccg gagcatccgc gaactgcgat cgaacgagat tctgatagag
    39121 agactcggca aaaaagggca cgaacggggc aatcactttg ctcagttcgc tcaggcacgt
    39181 ccacagtgtt tgatacgcgg cgttcttgtc ggcgtccatc ccgctgcgcc agaaccggtc
    39241 gcgcgagcgg cggacgtacc agttgctcag accgtcaacg aaatccacca gccgccggca
    39301 tgcggggtag ttttcgtagc gatccatcga ggcccggacg tcggcgatcg tctgatggag
    39361 ctcggccatg atccagcgat ccaactcgct cagtgtggga ccggcgttcc gccggcccga
    39421 ttgacaggct gaaaaccggc gggccggaag cccgctttgc accggagacc acttgtcgat
    39481 attcgcgtag atcacgaaga agctgtagac gttgtacagc ttgatgagaa actcgcgctg
    39541 atcggtggcg atggactcct ccttgaagcg cgcgccggtc cagggggcct gaccgctgag
    39601 catcgaccac cgcatggcgt cggccccttc gcgatcgaag atatacgtcg gctccttgta
    39661 attccgcttg ctcttggaga gctttttgcc gtcctcgccg cagaccaggc cgagcacgat
    39721 gcagttgcgg aaggggtgag gatacggctg agcactgagt gctgagtgct gggtgctact
    39781 cttctcccca aacaacatcg tgctgatcgc cagcagggtg tagaaccacc cgcgggtctg
    39841 atcgatggcc tcgctgatga agtcggccgg gaaccgctgc ttgaacaact cgacgctgcc
    39901 cggcttgtgg ggataacccc actgggcaaa gggcatcgcc ccgctgtcga accagcagtc
    39961 gatcacctcg ggcacgcggc gcatccgggc tcccggagca aaaggcgaag cgtaggtgat
    40021 ggcgtcgata tacggcttat gcaccttgag gtcgtcgatg agccccgggt tggcggcctt
    40081 ggccttttcc cagacgtcca tgccgctgac gccgggcttg gcggtcaatt cggcatatga
    40141 ggcgatcgcc tcctgcttgc ccgtcttctc gcactcccag atcggcaacg gcgtgcccca
    40201 gaaacgttcg cgggacaggg cccagtcaac gttgttttcc aggaagtctc cgaaccgccc
    40261 gtcgcggatg tgctcgggca gccagttgat cttccggttg ttggccagaa aggcatcgcg
    40321 gaaatcggtt gtccgaatga accagctctt gcgggcgtac tggatcagcg gatcgtcttc
    40381 cgctcgccag caaaacgggt agtcgtggcg atattgctcg cgtttaagca gcagtccctt
    40441 gtccttgagc caacgaatga tatccgcgtc ggcttccttg cagaatcgtc cggcgaagtc
    40501 cgtcgcctcg ggcgtgaact tgccgtccgg ggcaatcaat tgcaggaagc ccaagccctt
    40561 ttcgcgaccg atgcggtaat cgtcttcgcc gaacgccggg gcgatgtgca cgatgcccgt
    40621 acccgtgtcg agcgtcacaa agtcggccgt gatcacctcg tacgctttgc ccccttccgg
    40681 gcgggcataa gagaacaacg gctcatactc gatgccgacc aactcggagc ccttcacgac
    40741 cttgatagtg cgcaaatcct tctcaacgtc gactggcaaa cccttgaaaa cagatggaat
    40801 aagggccttg gccaggatga actcctcatc ttcgaccgag acatgggcgt attccacgtc
    40861 gggatgcaca gccaaggcaa cgttgctcgg caacgtccag ggcgtggtcg tccaggccag
    40921 caggctggtc ttcggccgac ctttgattcg gaagcggacg gtgatcgaag ggtcgtccac
    40981 cgcgcgatag ccctgcccga cctcggccga cgacaaagcc gtcccgccct gaggccacca
    41041 ccagacgatc ttgtggccct ggtagagcag ccctctgtcg aacagcgtct tgagcgccca
    41101 ccagacgctt tcgacatagg actgatggta ggtaacgtag gcttcttcaa gattgatcca
    41161 gaagcccaag cgccgcgtca gatcctccca ctccttggtg tagcggaaca cgctctgctg
    41221 gcagaggtgg atgaactttt cgatgccgta cgcctcgata tcttgcttgg tgtggatgcc
    41281 gagtcccttg cagacctcgg cctcaaccgg caggccgtgc gtgtcccatc cggccttccg
    41341 gtgacagtag tgaccagtca tggtcttgta ccgggggaag acgtccttga tgatccgcgt
    41401 caggcagtgc cccgggtgtg gcaacccgtt ggccgtcggc ggcccttcgt aaaacacgaa
    41461 cgatgggcgg ccttcacgct gcttcagcga tttctcgtaa atgcccgcct catcccagaa
    41521 ccgcgatacc tcagcctcag cttttggaaa atcgaattgt gtcggtactt ccttaaacat
    41581 aagtgcttat gccttcagca gttgcaaatc gctcccccgc ggctgtcatg cccaaggccg
    41641 gcaacgccaa gtctgcaccc ggccggggtg ctcgagttcg gaaagggtgt atgttagaca
    41701 gaatgctccc gactgccaac ggagcgacgg ccaactggca gacacagaat acctgctgcc
    41761 cgaacgccat cccgccaagc ctaagaggca tcgaggagtt gcggttgtct acctctcctt
    41821 cggtgtcgac gcctgcatcc agaagaagag atagtagatc tcgctggcaa actcgtagct
    41881 tccgggaccg cgatcccggt ctttccgaat cgccccgagt tcctccattc tacgcaggaa
    41941 gttatcgaac accttggcct ccgtgggtgt caatcgggca accgcgtcct tgcgggtgaa
    42001 tcgatgctca aacggttcct tggcaatctt gcgcaggatc ccctgatact tttcgctacg
    42061 gatcgcggcg gttaccttag gttcgacgta ctttgccccg atgacctcgg cgccacggat
    42121 gattccttcg agggcgtcat tcaggtcaat gtgattgtcc gcatcggtct tgaagacagc
    42181 atcgcccaac tcatgcatga aaacagggta accgccggag tacctggcga ggaggtcgag
    42241 aacgtcctcg tcgacctcag cgttaacctt ggagaatgct cgcctgaaga aatcgatcgt
    42301 ttccggctgg tcgaagcgac ggatctcaac gatctcgaaa acgcggtcga gcgagggttg
    42361 aacattcaca agctgccgtc gtcgttccgg gaggcccacg agcacaagcg tcagcggaaa
    42421 ggcgctcgcc gtggtcgcga actcatcgac caagctcttg agccagttgg caaatggagg
    42481 ggcggaggcc aggccgttaa tgtcatcaag aataagcagg ataccttccc gctcctcgcc
    42541 gaatttgttc aggaggttct tcatggtcgg aatgaagtcg ctgaccgccc ggttgagtat
    42601 ttgtccggaa gcggagaact caacagacat tccgaagata tccatctgct taatgtgatc
    42661 tccgagaaag tccttgatct tgccgaacca gggctggttc gcgctctcgc gcagaagacg
    42721 ctcgaaaatg cggcgagcca tttcttcaag agtactcacc cctccgagga agacgtgaat
    42781 gggtaggatt cgcagatcac gctccgccag cgactgcacg aaggtgcaga ttgagctctt
    42841 gccgatgccc cgttcgccgg tcacaaacag tctctcgatg gtcttctggg cgattgattt
    42901 ccggatgctc ccgatcatcg tctggatttc ctgggagcgg ccgatgaaga attccagcgg
    42961 aaccggaacg ccgggcgtaa atggcgaata ctccttgagc atggggcgtc ctcctgccaa
    43021 catggtagac ggaacactcg cgactgccaa tggagagacg atggtcgctt actccggcgg
    43081 gtttgtcgga tcagacaggg gcaattcgat cggggctgtt atgacaggga agccccctct
    43141 cggctcgttt cgcccgatca cgtcgaagct ctcccacgga ccccatcgcc accgagcgaa
    43201 caaggtgatg ccgatggcga taacaaatgt cgccgcgaaa gcccacggac cgaccgaccc
    43261 caggtccggc cgaagccggg ccaccagcac accgccgccg atcagaatga tccacacctc
    43321 gaccgcaccg acgatggacg accacatggt atcacccgct ccgcggagag cgcaactgtg
    43381 tgtgatggca atggcatcga aaaactggaa tacggccgca tagatgaata gtgtcgtgcc
    43441 caaccgaacc acggctggat caggactgat gaaacgcatc aaccactcgc cgtaaacgac
    43501 gaacaacaag gccatcaggc tcatgtaggc cgcattgaac gccgtgccga tccacgtgat
    43561 tcttcgagcc agcgtcgggt gtccctgccc aatggccttg cccaccgctg tgctcacggc
    43621 catgccgatg ccgacggccg gcatgaacga gacctcaagg taacgaaaaa cgccgccggt
    43681 ggccgccaga tccgccgtcc cgaaccggcc gatgatcgcc aactggaaga cggtccaggc
    43741 cgcgatgtcg gtgagaaacg ccgcaccggc cggccagccc acccagcata gccggcgcat
    43801 cttctcgcgg tcccaccgcc atgccttccg cggctggaat cttgatgccg ctgagctgaa
    43861 gtacatcacc gagaactgga aaaccgcgcg gacaaccgtg gccacgaccg tgccccaggc
    43921 cgccccggaa acgcccatgg caggaaaacc cagcttgccc agaacccagg cataggtcag
    43981 aacgacgttc accgagacac tgaggaaggc cgaaacggtg ttgatccccg gacggtggat
    44041 tccgttaaag aagttgtcga gagagatcgt tgcggcggcg attccgaggc tcagcaagcg
    44101 aatgtggagg tattgcacct ccatggatgt aacggtcggc tcgtgaccga acatctgata
    44161 gaacgcaggc acgaacggcc agagcatcag ggcgcccatg ccgtacagca gtgccatcca
    44221 gataccctgc caggcgtagg cggcgcagtc gcgttcacgc cccgccccca gcgactggct
    44281 gaccatcgtc gtgacgcagg tcatcgtccc catcggcatc gccagcaggg tgatgaacgt
    44341 caggctcgcg gaggagatgg ccgcttgagc ctcggaagcg gccgacatct gcttgacgat
    44401 cacgaagtcg gtgaagctca tcagcgtctg agctaccgtg ctggcgacgt tcggcacggc
    44461 cagcttgagc agcacggccg tgtcaaaccg ccaactgcgg cccagcagcg ccggatcatc
    44521 gccgccggtg cggccacaca cgactaaggg aaccgcttcg tcagagggca tcactcaagc
    44581 tcgaaacaaa aaccccggaa gacacgttgc tcccggggca cggatttccg ctttgtccat
    44641 tggccaacag cgttgctgtg accggctgtg gcgccgaatc atcgccgcac cggaggcaga
    44701 gtttttaccg ggctcggagc atgcctggtc cggagtacac ctcgtccacc tggtcacgct
    44761 gccgcggagg gctggccgta acgttatccg caggctgtgg ggctgtcgcg tccggttcag
    44821 gcttgggttg ggctgccgtc ggttcaggat tatcctgagc ggttgcggct cggtccgtcg
    44881 gtgcctcggt cgggttttcg ttgtccgcag cgggggtatc agcagccccc ggcgcctcct
    44941 tgcccccaag atccggcaac ggttgggcgg aggccttaat ggaactgtca agagcttgca
    45001 cactgacagg tacgggcgcc aatgccgggc cgtcactccc aatgtatcga ggcggcggat
    45061 ccgaattgcg cccgcaaccg catggcagta gcagacccag acataggagc atcaacgcat
    45121 gccgaatcat tctgaggcat cctccccggc aggtatccac aaccgaagag catacggacc
    45181 cgaggggcgg gcggtcaagg caacccgatg attcgccgtg aggcagggct cgtgctctct
    45241 ttgtccgcca cgttctgcgc ctatgccccg atggcttgcg gccttcgcgg ccaaagggcc
    45301 ctgggggggc gaaggagcgc tgcccgccac ggcaggcccg gtcggcgagc aacacagcgg
    45361 tcctgcggct cgatcaaaga tgtcgtgcgg tcgcgagctt ttgtgtggaa atactttcgt
    45421 tgccgcaacg ttacgcattg tagataagag acttgcgcgc cggcacttat cggaacgacg
    45481 acggcggtca aaggtcgcgt ccatacttga agtgcggtgg gcaaccaacc ggaatgctgt
    45541 gctatgaaca agcccctcct cgctgccgct gtctgcgtga tgtccggcct cgcgtctggc
    45601 gaggagccgc ggatcatccc gccgtttgaa gtggagcggc cggtcgtatc ggcgaacgcc
    45661 atcgacgacc tggtgtggcg cgccctgaag aagcgcggca tcaaacccgc gaatccgtgt
    45721 tcggacgagg tctttgtccg gcgggtttac ctggatgtca ttgggacgtt gcccgacccc
    45781 gttgaggtca acgagtttct gcaggaccgc cgtccggaca aacgagccgc actcatcgac
    45841 acgctgctga gccgcgacga gttcgcggac tactggtcat tgaagtggtg cgatctgctg
    45901 cgggtcaagg cggagtttcc gatcaacctc tggcccaacg ccgtccaggc ctatcatcgg
    45961 tggatccgcg actccattcg cgagaacaaa ccctatgacg agtatgctcg ggagctgctg
    46021 acatcgagcg ggagcaattt ccgcgtcccc ccggtcaact tctatcgagc ggtccagggt
    46081 cgtgagccct cggccattgc cgcggcggtg gctctcacct tcatggggac gcgactggac
    46141 gattggccgg aggcacgccg cgccgacatg gccgtcttct tctcgcggat tgcatataag
    46201 aaaaccggag agtggaaaga agagatcgtc tgcctggatc ccgcaccaac cgggcctctt
    46261 gaggcggttc tcccggacga cacgcgaatg cggatcccgg cggatcagga tccgcggatg
    46321 acgtttgccg actggctgat caaacccaac aacccgtggt ttgcccgcaa catcgtcaat
    46381 cgcgtctggt cgtggctgct gggccgcggg atcattcacg aacctgacga catccgtccg
    46441 gacaacccgc cggtgaatcc cgaattgctc gcccgtttgg agaaggaact ggtgcaagcc
    46501 cggtacgatc tgaagcacgt ctaccggctg atcctcaatt cccgcaccta ccagcagtca
    46561 tcgatcccgc gcagcgaccg tccggaggcc gaggccctgt ttgcgtgcta cccggttcgc
    46621 cggctggatg cggaagtgct gatcgacgcc ttgtgccggc tcttcgaatc cacggagagt
    46681 tacatgagtc ccattccgga accgttcacg gtcattccgg ggtatgagcg caccatcacg
    46741 ctggcggacg gcagcattac cagtcagttc ctcgaaatgt tcgggagacc gccgcgcgat
    46801 accggcctgg agtcggagcg caacaatctg ccaacggaag cccagcggct tcatctgctc
    46861 aattccagcc atattcagaa gaagatacag aacagtccgc gcttgcggcg attaatcaac
    46921 aacagccgcg gaaaccgccc tgctctgatc cgcgtccttt acttgaacat tttgtcgcgc
    46981 tacccgacgc cggccgagct ggccgccgcg gaggagtatt tccagacccg aggagtgaag
    47041 cccgaccagg cggtgaatga tctggcttgg gccctgatca acaccaaaga gtttctatac
    47101 cggcactagc cgggccggat gatgatcgtg aggttgcccg aaatcgactg aggaatccca
    47161 cgtgagcgga caatacccgg aaaagatgat gacgcggcgc gccaccctga agcaggggct
    47221 gttgggtgcg gccgggctgg ctttcctgga tcatgaaaaa gcctgggcac agcccgcagc
    47281 ccaatctgcc cactcgacga cgaggccggc caaagccagg gcggtcattc agatatggat
    47341 gtggggtgga ccgtctcacc tggacacgtt cgatcccaag ccggacgcgg gcccggacta
    47401 ttgcggccca ctgaacaaac cgattgcgac caatgttgac gggatccgaa tcggcgaatt
    47461 gcttcccaag ctggcccagc aagccgacaa gtactcgatc attcgcagca tgacccacgg
    47521 cgtgaacgct cacgaaacgg cgtcctacat cgtgcaaacg ggacgcaaac ccgaccggct
    47581 cgtctatccc tgcgtgggtg cggtcgtgtc ggtgttcaag ggatatgacg gcggctacaa
    47641 ggggctggtt ccaccatata tcgtcctgac cgaaccgcag ggtcgcttct cggaggcagg
    47701 ttttctggga cagcgttaca aaccgtttgc cacgggcggt gatcccaacc aatcgcgatt
    47761 tgtggtcgag ggcatcgtgg cggaaggcat ttcggacgag aggcagcata gccggcgcca
    47821 gttgttgcac aagctcgatt cactcggccg ggccatgccg gcaaacgctc agttcgccca
    47881 gctcgaccgt tgcgaggaaa aggcgtacga cctgatgttc ggagaagccc gcaagctttt
    47941 cgatctgtcc agcgagaagg acgagatccg cgaacgttac ggccgtacga ccttcggcca
    48001 atcctgcctg atggcccgcc gactggtcga aagcggagtc ccctacgtga ccatcaacta
    48061 caagggctgg gacacccaca aacaacactt cgagacgatg cgccgcaagc tgccggaaat
    48121 ggaccagggt atggcctcgc tgcttcaaga cttgtccgat cgaggcctgc tggacggcac
    48181 gatcgtctgg tggagcgggg agttcggcag gacacccaag gtccagtggg aagcgccgtg
    48241 gaacggcggc cgcgggcatt acggcaactg cttctccgcc gtggtcgccg gcggcggatt
    48301 caagggcggc cgtgtcgtcg gagcctcgga cgccaagggc caagaggtgg ccgcccgacc
    48361 ggtttatccg caggatctga tccgcagcat gtacgagttg ctgggcatcg atcccgacgg
    48421 cccgctgccg aacgcgcgcg gcttagccgt gccggtgttg ccggccgctg agggtgacgc
    48481 acagggaggc ggtcctctca aggagatcat gtgatcacag ggagcacggc catgaccata
    48541 tcccggcgat tgccgctggc ggcgataacc ctctgggccg ccaccgcgtg gggccagtcc
    48601 agccagagag aaccgcatat cggctatctc taccccgccg gcggccaaca aggcaaaatc
    48661 gttttgatca cggtcggcgg tcagaacctc aagggcgtca ccgacgtcta tgtctcgggc
    48721 agcggggtgc gcggcacggt cgttcagcac tttcgaccgc tcaatgtcat cagacaggag
    48781 caacgagaag ctctccaaaa gacaatgagg gaactgattc agaagaggat cgccgagcta
    48841 cccggcggcg ggtatgactt cacggcgctc ggccgggaat tcggcatcgc caggcccgac
    48901 gtggtcgggc ccacggccgc acgaaggcaa gacgccccgg ccccgaagaa ccctcccaag
    48961 aaagaccggc cttccccgca ggcaaagccg gacaaccagg caaaggcgcc ggaaacacaa
    49021 cccgccgagt tgccggaaca tcctctgttg tacgagcttg agaaaaagag tctccgggaa
    49081 ctgttgcaca tcagacgtga gttgctgaat ctccgcaaca ggcagctcaa tgcgcagatc
    49141 gccgagtcag ttctgattcg agtcgccatc gaacccaacg ccaaacccgg cgaccgcgag
    49201 atcagactcg gaacacctct cggcctgacc aaccccatgt gcttccaggt cgaccagctc
    49261 gcggagatca ccgagcttga gcccaacgac ccgcatgccc ccagtctgct gcccgatgag
    49321 ccgcccgccg ccctgcctgt catcgtcaac ggccagatca agccgggtga cgttgatcgt
    49381 tttcgctttc gggcccagag gggccagaaa ctggtgatcg agacctgggc ccggcacttg
    49441 gtcccatacc tggctgacgc cgtccccggc tggttccagg caactttggc cttgtacgac
    49501 gcgaggggca aagaagtggc cttcgctgac gattatcgtt tcaatcctga ccctgtcttg
    49561 atgtacgaag ttcccgatga cggcgtgtac acggtggaaa tccgcgattc gatctatcgc
    49621 ggacgggagg atttcgtcta ccgcatcgct gtagcggaac gtcctttcat cacccgggtc
    49681 tttcctctgg gtggccagat gggcgtgcga acggccgcct ccatcgacgg ctggaacctt
    49741 ccggccaagc agttgttcct ggccaccgcg gggagcaccg acggtatccg gcaaaccgcg
    49801 ctgcgccgca acgacaatcg ctcaaacgaa gtcagttacg cggttgacac gttgtcggaa
    49861 tccttcgaga ccgaacccaa cgacacacct gagaaggctc ggcaaatcga actgccgcgc
    49921 attatcaacg gcaccatcag ccggccgggc gacgtcgatg tgtttaagtt cacgggcaag
    49981 gccggaaccg agatcgttgc ggaagtctgt gctcgcaggc taggctcccc gctggattcg
    50041 atgctgcgga tgaccgatgc cttgggacgg gtgctggaat ggaacgacga tcatgaggat
    50101 aaggaatcgg ggctcctgac gcatcacgcg gattcgtacc tgcgcacccg actgccggag
    50161 gatgatgtgt actacgtgta cctgtctgat tcccagcacc acggcggaaa accgtacgca
    50221 tatcgcctgc gcttgggggc tccgcgtcct gacttcgctc tgcgcctgac gccctccagc
    50281 attaacgtgc gtgcaggcgg caacgccatg gtgacggtcc acgcggtccg caaggacggg
    50341 ttcgacggcc ggatcgaagt ggttctgaag gacccacctg caggttttgc cctcagcggc
    50401 gctcaaattc cgtccggccg cgaccaagtg cgcatgactc tcacggcgcc tcaaataccg
    50461 ccggataagc ccatttcact gcaattagag ggacgcgccg aggtcgcagg ccgagtcctg
    50521 acccgtccgg tcgtgcccgc cgaggatatg atgcaggcgt tcagttaccg gcatctgacc
    50581 ccatcgcagg agctgatggt tgccacgatc ggcggcagac gcggcggacc tcccatcgaa
    50641 gtggtcaaca aagggccggt ccggattcct tcgggtggaa cggccgaggt gcaaatcaag
    50701 acgcccgcct accggatgca acgagagctg aagttcgagc ttcgcgagcc gccgcgtggc
    50761 gtcacgcttc aggacgttac cggggcacct cagggcctca ggctggtgtt caaagccgat
    50821 gcgagtgctg cacaggtcgg gtttgcggat aatctgattg ttgaggtctt cacggaagct
    50881 cctgcgcggg taccaagctc cggtgggcag aaaaaatcgc gagcgtttct cggtgtgttg
    50941 ccggccgtcc cgatcgagat ctcgccgcga tgagaggatg tctttgaaag aggtcgcgga
    51001 tggatttcta tgcacatcac tgcgggatac cctcggtgtc ggtcaacatc tcgcggtttc
    51061 ggggaccaag cggggttgat gagtatcact tggtcgtgac gcccaccaaa tatgggagcg
    51121 tcaatgccca gttggagtgg gtggctcacg cctaccacaa gaccctggaa tcgatcggac
    51181 tggatgcccg gacggcggtc ctgcgacgat tcttctgcag cgatctgcac aaccaggctg
    51241 aggcactcga tgcctgtccg atttcacgtt gcggcaacgc ggacgatccc tgcgccattt
    51301 cgcgggttgg ccagcccccc ggtccgccgg caaaagtgat actatgggct tatcacgtac
    51361 gtgaccgcgg caacgaactg gagaaaagcc ttcgaggcgc ccatttgacg cttcggcggg
    51421 gagatttatc gcaccattgg acgaccggtc tgacctgcac gaccggccac ggttcatacg
    51481 gtcagacgcg cggcatcttc gatcggtaca acgctttgct atgcgaacag ggcatgactc
    51541 tggccgatca tgtgatgcgc acgtggcttt tcgtccggga catcgacgcc aactaccaag
    51601 gtctggtcgc cgcccggcgg gagttcttcg cggaacatgg attgactgcc gatacgcatt
    51661 tcatcgccag ttccggtatc ggaggctcca gcgcagaccc cgccgcgatt gtgaccctgg
    51721 acgcctacgc catttccggg gtgcggccgg agcagatccg gtttctagcg gcgcccgatc
    51781 acctcagccc gacctacgtc tacggagtaa ccttcgaacg agggacttcc gtcgcctacc
    51841 gggatcgcaa gcatgtcatc atctcgggca ccgccagcat cgatcaccag ggcaagatcc
    51901 tataccccgg cgacgtgtcc cggcagctcg accgcacgct cgagaacatc gaggctctgc
    51961 tcgaccatgc cggcgcaacg gtgaacgata tgagcgcatt catcgtgtac atccgcgacc
    52021 cgagcgacca catgattgcg cagcaacaaa tgcgcgaccg ttttggccac gcgccgattg
    52081 cggtccttgt agcgccggtg tgccggccgg gatggctgat cgaggtcgag ggaacggccg
    52141 tcgttcccga aaccaacccc gaactgctgc cgttctgatt acatgacccg ttggtgccac
    52201 tccgcggtac gttgaagagc acttgtcgca ggccgcctgc ccgggctgat ttcggccggg
    52261 cggggcgttc cagcttggcg gccggagcgt ttcgcgaggc gagcgcggca gcctgcactg
    52321 ctgcgccggt gaccgtcaga acttccagtc aagcccgccc ttgagcggtt tcttgcgttt
    52381 tttgggctcg gcggccggct cggtcgaggc ggtacgctcg ccggtgtact cgggtacgct
    52441 ggcaagctgc ttgatggaaa ggctgatgcg gcggctttct tcatcgacct ccagtacctt
    52501 ggcctggact gactgtcctt ctttgacgac gtcaccaacc gagcgcacac gctgatcgct
    52561 gagttcgctg atatggacca ggccctcgac gcccggaatc aactcgacaa acgctccgaa
    52621 cgcagcgatt cgcttgaccg tgccggaaac gacgctgttg accggccagc gaaccgatgc
    52681 ccccacccag ggatcgtcgc ccacctgttt gatcgacagg gcaagcctct tggccgcggt
    52741 gtcgagcctg atgacccgaa cccggacgac ttcgccttcc tgcaacacgt ctttcggatg
    52801 cgcgattcgc cgctcatagg tcatttcgct gatcggcacc agcccctcga cgccgggctc
    52861 caactccacg aacgcgccga aatccatcaa tcgcgtaaca cgacccgtga tcactgagtc
    52921 caccggatac ttgacttcga tgttcaacca cggatccggc atcacctgct tgaggcccag
    52981 gctgatcttg cgggtgtcac gatcgatctt caggaccatc agctcgagct tctggccctc
    53041 cctgacgacc tctttgggat cctggacgcg gctgtaactc atatccttga cgtggagcag
    53101 accgtcgacc ccgtcgccca gcgaaacaaa cgcgccgtag ggcatgatct gccggacgag
    53161 gccggtgacc gtctgacctt cgaccagcgt cgcatatttc cgctccctcg actgttcctc
    53221 ctccagccgg agcacctcgc ggcgggatac gatgatgctc ttttcttcat ggcgcacctc
    53281 gatgacctgg cacttgagcc gccgattgac gtagcccggc aggtcggtgt cctcgacccg
    53341 aaacagctcg atctggctga tgggcataaa ggccttgacg ccgttgatgt ccatttcaag
    53401 gccgcccttg ttgtggcccg tgacccggcc ttcgacgatg tgcccttcct ccagcctcgc
    53461 ccaggtcgtc gccatgacgg cacccttgcg agaaagctgg agaatgccct cgctgcgatc
    53521 gtatccctca atggtcactt caaccacgtc gccgacgcgg ggaaggggtt catcctcata
    53581 ctgcgtggcc ggcagcactc cctggctctt gccgcccatg tccacaaaga tgtcgtcgcc
    53641 gtggatggcg atgacccggc ctcgcttgac cccttcggcg ctgggcaccg cgaaggggat
    53701 gccccggggg atgtccagat cgatcaggct ctccagactc atctcgccca gagcctcgtc
    53761 cagttcctcc tcgaaacccg cctcaagggg gctgtccaga tccgaatcct cgtcatggcg
    53821 gtcatccgcc gcactgtcgc tcatcttggg ttcctgttct tggtgcggtt cacggcccgg
    53881 ccccgttctc gccaagccgg tacgtcgcga taggatagtc aatccacgcc gtcaaaccaa
    53941 gagtgcccgt tccaggtacc cgcgtctgag gtgatgacgt cagtctccac agtcggaggc
    54001 tggaaccgta ccgggaccgc tgaaacaccg ctggaacgtg gcgaaatcag actgatcgac
    54061 gtcccgatcg ccgtccaaat cggctggatc gcagtccgct gcagatatcg gaacgccagg
    54121 acccgtcgcg catgcctgga acaggtcata gtccacgtcg tcgacgtcga gatctccgtc
    54181 gaaatccgct cctggtcgcc gggtaatgtc ctcgatagcc cagtcgtcca agtgaagcga
    54241 gtggatcgcc ctgaccgtac ccgcaaaacg ccagaagaca atgacggtaa ggagatcctg
    54301 gcgcggcgtg atttccgctt gaagttggac ccacaccggt gccgcctgac cgtcaagatg
    54361 agcccccagc acgctgtggt tggtgatgtt taccccacca ttcaggcaat tcaagggcgc
    54421 cccgtcgccg tctcgcaccc gaagctccac gaaatcgtcg aagtactggc cgcctgaggc
    54481 ggtgatcgcg gcccagaaac gaacccgata ggtgtggctg ggccgcgccg ctatcgtctg
    54541 ccagatcgcc ccggggccga ggctgtcgcc ggcccgatcg gatgacatcc caacgctgtt
    54601 tgagccgtcg tgaaaccgcg gcgagggaat gtgcgttgag ggattgtgtt tgatcgagga
    54661 ctcacccccc aggcaactcg accagccggc cagaaaattg cccgcatcct caaatgaggg
    54721 attgacgatc tcagccaccg ttccgacgcg gatgtaatcg gttttttgca gggcaattgg
    54781 cccggcggcg ccggtgacgg caagccggac cgtaaaacgg ccgtcgtaga gatacatgtg
    54841 ctcagggtgc tgggcggtgc ttgtctgacc gtcgccgaac tcccagaacc aagccgttgc
    54901 accatggaca catgaacgat cattgaaatg aaccaacatc ggcggctggc caaccgtctt
    54961 gtcggcaccg aaatcaacca catcgtcaaa gttcttgttg aagtctttga acgtgtaaaa
    55021 cgatggtttg ggattgaagt attcgtcggt cagcccgaag caacagaaat cattgtttgg
    55081 gttctcgtcg tggttgttcc agtcgttcaa gacgagatac ttggcgaaaa aaacgtagct
    55141 gtaatccgtt ttcttcagct cggtcaacac ctcgttaagg tcgtccgctt tctgagtctc
    55201 gttgccagtg ctccagccgt actcggtgat ccagatggac ttgtccgcat caccgtggtc
    55261 aaccatgacc tgacggacac tagtcaggtc gctgtagtcg atgccctccc cggaggttcc
    55321 ggggccgccc ggaccgccag gggcataggg gtgaattgaa acagcgtcaa acgctttctg
    55381 gcccgcaagc tgggcataca tgcagttgac gtacccagcc ggatcgccgt cgaagcctcc
    55441 aggcgacacc acgcaatccg gatttccagc tttgagagcg tcgtaagctc ttttttgcca
    55501 aagggtaaag agcgaacaat cgtctccgtt caggcagttc tcgttgatcc aaccacaacc
    55561 gctgggttcg ttccagaact ggtagtaggt cacccgaccg gcataccggg cggccaaggc
    55621 cgagtgaaaa gcttgaaaag cggtcaagca ttcctcgctt ggcggcgttt ggtaattcgg
    55681 caaatgagga tagagcttgg cccactcagg cgtggtgccc acgaaggcga acatctgaag
    55741 accgtgggct tcggcggtgg ccacttggtg atcagcgtac gaccaatcaa tggtcccctg
    55801 gaccggctcc atgctgctcc aagggaagtc cacccacact gccgttgccc ctatttcgac
    55861 cattttatcc atggcctggg agtcgtatga ccagggctct tctgcccact tggccaggta
    55921 atggcccatg ccaaagacat aagggcgaca ctgggcgagg accgcgcggg gaccgaccga
    55981 ggaaaaaaca aaagccgcca ggactgataa tgagactgtt ctcatcactc cctagcaccg
    56041 tcggaagggg cacggcgatg ctccgaatac cccgggcccc tgcggtccat tctacaaaac
    56101 accgcctgta aagtggaggc gcaatcctgc ggcgactggt agtgggtctg ccacgggaca
    56161 cggcggggtg cggccgccgg cttgctcgcc ggtaacccct gctcctgcgg catacacccg
    56221 gggacaagcc acccgcggca caggaaccat gctcctcccg accttgtccc ggtcacttca
    56281 accaagtctc ggacgggcac gtgccagtcc ccgcttggca caaatgccgg tcctacacgg
    56341 gtctatccat gtcgtcttgc gttgacggag gtgggggcga gcctgtaatc ttgttttgct
    56401 gccaaggagg gtcggccctg cctgggctgc cggccgtgac cgcggcgtca caggaccggg
    56461 ctgagtccgg caagaggacc gaacctggtc cttccgcacc ggatgcatgc cagatgattg
    56521 cctgcgtggg atgccccaga agcttcctca gccggaggtt catgatgagg aattggtgga
    56581 tgcacggcct gtgcccggtc cagatgcgtc tgttcgtgat cgttgttatc tgcatttccg
    56641 gagtcatcgc tccggcgccg gcccaggaaa cgaccgcgga gcccgcgccg gcggcagccg
    56701 cggagcctga cccaagagac gccacgctcg aatcgctgtt cgcgaactac cttcacttcg
    56761 ctctgattgg ccgattcgag gtcgccgatc aacgatttgc ccagcctctg ctgagccggc
    56821 ccgagctgaa ccccatgacg ccggaggctg ccgagacgct catccagctc tccgagaaat
    56881 atgagaacag catggatacc ctgctgctcc tcattaacaa aagcacaatc ggcgagaacg
    56941 cgcagaaaat catgaatctg atccgcgagg cgcacacccg caagaggatg gaccccgcgc
    57001 ggatcaaggc caacatcgac ttgctcaagg gcgaccccgt gcaaagggcc gtcgccgtcg
    57061 aaaggctggt cgaatcgggc gagtatgcgg taccgtggat gctcgggctg ctcagcgacc
    57121 ccgatcggga ggatttgttg ccgtttatcg ctcgggccct gccgcaactg ggccgcaacg
    57181 cgatcaaccc tctgctcgcg gctctcgact gcaagcatga acgaaccttg caagtcgtca
    57241 tcgagtcatt gggcaagatc ggctatcctc aggcgctgcc ctacttgcag aatctggcga
    57301 ccgcaaagga tacacccgcg tcgattcaga aaaccgcggc cgaggctgtt gcccagattc
    57361 tttcgaagaa ccagggcatt cagctcaaac cggcggaaga gttgttccgc gacctggccg
    57421 agcagcattt tgcggagatc gactcgctgc gaccggaccc ccgcgaggac cgagccaacc
    57481 tctggcgtct acgcgcagac gcccgacccg gcgaggacgt agttgctcct gtcgccgtcg
    57541 attccaggat ctacaacctg gtgcgatgca tggagtgctg ccaaacgtcg ctctcgctga
    57601 agaccgacca gccggcggta ctggctttgt gggtggccgc caacttccgc agggaagccc
    57661 ggctgggtct ggacgtggaa tcccaagaga cggttgatgc tcgcaagctc gatccgtcac
    57721 gacccgagaa tttcccgcga agcatctatt tcgcgcggat ggccgggccg tcgtgcaacc
    57781 agattgtgct ggcccgaggt attaagcagc gcgaccggaa catcgctctg ggggcggtga
    57841 cggccttgaa cgcgaccgcc ggccgacagg cgatgaccga cgttcaagcc gagggcggca
    57901 ccagcatggg cgaggccttg tacttccccg atctgctggt gcgcgtgcaa gcggccttgg
    57961 ccctgggcaa gacgctgccg ccggcacctt tccacgggtc caccgaggtt gtgcccgtgc
    58021 tggcctcggt gctggcgccc tctgagaaga aaaactacat gctggtggac ccggaagcgg
    58081 aatcggcggg cgtgatccag acgggtctgg tcaacagcgg ggcggtcttg gttctggcgg
    58141 atcggctgag cccggcgctg aaccaggcac gtcgcgaaat gacgcatctg gacggcatct
    58201 tcctggcgag cgacatgaag cagcccacgg tcaccgaggc catcgagcaa ctggccaaag
    58261 atgatcgctt ctgcctgact ccgatcatcg tgctggtcaa ggagaaccag actgcggtgc
    58321 tcgaccggat cgatgggtcg gacaggcggg tcggcagcat cttcgtcctg acgacgccgg
    58381 acaagaccac cgacccgaag atcgtcgagc agttgctggc caagctcgag caggtgacca
    58441 tccgctgcgg cttgcgacct ctatcgcccg agtcgaggct ttcgctggcc ctgcaagccg
    58501 ccggggtgat caagggcatg gctcagagcg aatcaaccgt tttcaatgct caggcggccc
    58561 tcccggcaat ggtcgaaacg ctccgccagg ccggcgcgga agagcttcga gcagcggtcg
    58621 ctgcggcttt ggcttggatg cctgctcccc aggctcaaca agccatcgcc gggattgctc
    58681 tggccgccga ggagacggaa tccttgcgga tgacgacctt cggcttgctg gccgattcgg
    58741 ctcgccgctt tggcaatcaa ctggacgata gtctgatgtc gcggctgatg gagcaggcga
    58801 ccagcgagcc caatctgacc ctccgcacgg ccgccagcca agctatgggg gctatgaatc
    58861 cgcccgtgga gaagattctg cctatcatca cgggtaagtg accaaaaact tgttcttgcg
    58921 gggtgaaaag ctgagatcgg tcccaagctc aagatgcgga ggcgttggtc cccgtgggcg
    58981 aaccccaaag gcagaccgcc ttcttgcggg ccgcaaccca aacgcaccgc gatccggaaa
    59041 catgcccgca agccgcacag acgtccggcc gtaaggttcc tagtcctcct ccgggcctgc
    59101 cgatgctccg ctcttcgcca gatccgacac gttgatcttg tccgtcttga acttcgtcgc
    59161 cgcctgctcc atcttgtcga gcgcctcctc aatcggcatc gtcttaccct tgagaaagcg
    59221 aatcgggttg attctcgcga ctacgaagct gcgcagatat gggctctcca aacccctggc
    59281 tttcagttcc cccacgatct cggtcacgcg ggaatcgatc ttcaagagct gttcggcata
    59341 gccggcccgc ctggccagac tcttgggcaa agcttggttg aggaatacat ccacgcgctt
    59401 gagcatcggg tggtaggctc cgccggcgaa acgcccattc tgttcgtagc agaggccgag
    59461 cgtcaggaat gcagcttcct cgaactccag agcgtagtcc tcctcacggc cctcgggcat
    59521 ctcggctagg tgccgggcca agcggatgac ctcggaagcc ttttcacgaa ggttgtgggc
    59581 cttctcgacg ttcagcgcca gaatcagccg ggccacctgc ggctcagtga ctaccaaggc
    59641 ggtgatggac tgtgccccct gattcttcat cgccgtggtg cggtggtggc cgttgggcgt
    59701 ctggtaccgc ttgggcccga cacggacggc gatgaccggg tcgagataca ggccgctccg
    59761 cgagatgacg tctgcgaggc gtttgacgtg cgtctccgac aatccgcgct ggtagggagt
    59821 tggctcgacc tgctccaacg gcagggctgc caggatcacc caccggccgc cgagcggttc
    59881 gcggtatgcg gtcaacaccg cgccgccgtc cttttcgata tccttgcaga gcgactcgac
    59941 ggcactcggc ggctcggccc gcacctgcga cggcttgagc ccgaccggcc cggccggttc
    60001 actcttcttc cttcgttttc gtgttttggc tgcggccatg gcgacgtgcc tcccgtcgtg
    60061 gaatccgaag atgccaggag ctcacgtcga cactatccac ccccaagagc ggtcttgtca
    60121 agcggcactt gcaaaccgcc tcgccgcaac ctgttggtgg agcgcgtgca ggcatgatgc
    60181 tgtatttgta ttcgcacaag cggaatctat ccgtggagga cttgacaact ctgggagtca
    60241 ctgtatgctg gtggtcggca ggttgggttc cggccattag gaggcggcaa ggcttcactc
    60301 cgtgcggggc cgggcggtcc ctggagggct aaagcgttgc cgatcgacca tctgaacggg
    60361 aagtcctttc acggaacacc cgaggtgcgc accggcgtgt gcggcctgcg cttcgcgtgt
    60421 ttgtgaggct catcccgcaa ctgtcccggg caccgctcat ttcgtcctgg tgtgatgagc
    60481 ggacgtccga ggacccggga tgaggaggtg aaaagcatgc aggcaactcc ctcgttgcgt
    60541 ctccgtcggg cctccgcgcc tccactcggt ttctgtgccg gcaaaacagc gacggtcaga
    60601 atagtctctt gcgtcgggac gggtgcgcac tctgcgcgca tgtttctgga tcgcagagcg
    60661 cttgggttgc ggcccgcaaa aaggcggtcc gccttacggt tcggtccgaa ccacaaaggc
    60721 ccgaccaacg ccctcgccgc tcccaccaac cagtccgccc catcaagcat cagacgcaac
    60781 aactctgaag gcgaaagtat ttactcggag gccaccgacg atacttggcg gccggcgcga
    60841 tactgcatcg gcaaagtgcc ggtgcggaac aacacccaac acgaggagga actaccgtga
    60901 ggaaagcaag tgggcttcgg gaggcttggt tgcggattgc tgccgccaca ctgtgcattg
    60961 ggaccagcta tgcaggagct gtaacgatcg atttcggccc gagtttcggg gctccatcct
    61021 cggattggga taatcctgac cgatcagctt tctgcctggg gagtaacgtt ttcgacgccg
    61081 agccctgacg gcgtttactg gtggggaccc ggcgtctacg gggcctatag catcagcgaa
    61141 ggtcccacgt ggggtccaaa cccgcacgga accgatccca ttcgcatgaa tttcagctct
    61201 tatgtcaatc tggtcgccat acgcggcttc aacggcggcg gcgacaccga cacgatgatc
    61261 ctcagggctt acaaccatgc aaatcagctc gttgccgcca agcagatcac gagcgtattc
    61321 agtacccagg gtcacactct cacggttacg gccccggaaa ttgcctacgc gaccgtccag
    61381 gcggttgctc cgctgtcggg gttgttcttt gacgacctga gtttcatctt cgtcccggaa
    61441 ccggcgacgg cactgatgac gctcctcatg ggttgcttcc tttgcggaca ctggcaccgc
    61501 cggcggcggt gagcgatctg cggccccgaa tgcttgggaa gcccggcaag gcagcgcccc
    61561 gcaagacaat cgccggcagg agtgtcgact agggcgtcgg caccaccgcg ggcggtcagg
    61621 cgcttacgcc ggatccgtgc tgctgcccag gtctttccaa tgcacgaccc gcggctcgta
    61681 cgcgttcctg gcagcgttaa tcgcggccat gcgccccatg aggcacatca gggtggcaat
    61741 gcggccggtc tcgacgttgg catggatggg cagtttaccg tccgccttga tgttggcgac
    61801 gaaagcctgc aattgctcct ttgtgcccat gccccaatcg ccgcgcggat cgtcggcata
    61861 ctgatctttg agttcttcct gcggcgcggc tgcggtcttg ggatcgtggt cggcccagtg
    61921 catcaccccg tgggccagat ccacgccgcc cttttggcag tgaacatgca gttgctcggt
    61981 ctggaagtga cgagccagcc acagcaggtg cgtataggag aagatttcgc cgcccggcca
    62041 ttgccagatc gtcgcggact gctgctcgtt gtgaacgttg ggaccctcgg gcgtgggaat
    62101 ctcggttcgg gcaatggcca cgcaagtgat cggcggcttg tcgcgcatga cccaggacat
    62161 gacgtccgta tggtggcagg cctgctcgac cagcatgccg ccgtcgcggg ccacctttcg
    62221 aggctccttc ggccggtggg cccagtgcca gtggccctgc atgaacatca ccttgccgag
    62281 cgtgcccccg tgaatcagct cgatgccggc caggtgcggc gggctgtagc ggcgctgggt
    62341 gccgatctga tatatgacgc ccttggcctt gcgcgcagcc ttg
//
LOCUS       DAOTPW010000002        51579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_726_length_51579_cov_5.633724, whole genome shotgun sequence.
ACCESSION   DAOTPW010000002 DAOTPW010000000
VERSION     DAOTPW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 51579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51579
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_51579_cov_5.633724"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="P5316_00225"
     CDS             <1..259
                     /locus_tag="P5316_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83404.1"
                     /translation="FRLATPQPLGQRGPSMSHWYLGIGHSDFIGHSGFDIGHQDFDFR
                     RSDFGIPLPPLKSQILKSQIPPTPFPVVSGGVKMQKSRRAG"
     gene            complement(774..1565)
                     /locus_tag="P5316_00230"
     CDS             complement(774..1565)
                     /locus_tag="P5316_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRR83405.1"
                     /translation="MCASCTSRLPLGSPDNVLIIAHRGNSAVAPENTLSAFRSAVAAG
                     AIYVELDARPSADGTLYVLHDATLDRTTDAKEVFKREKIKIREIPDAALDKLDAGRWF
                     DAQFAGERLPKLTEALDVIQAGSLTLLEHKDGPAEDYVRLLRQKRLVGKLVVQSFDWE
                     FLKQMHQLEPRQVLGALGDKELTEERLAGIAAAGVRIVGWKHTDLTAPLVCDLHGRGY
                     RVWAWTTNEPADWQRLLGFGIDGIITDRPAQLAAWLAQQTVTSHR"
     gene            complement(1694..3976)
                     /locus_tag="P5316_00235"
     CDS             complement(1694..3976)
                     /locus_tag="P5316_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR83406.1"
                     /translation="MRTEPQATIPTEKMCEKGSPTLNGTAGLDRRGDLDAARPHARGG
                     FHTHSGSFASAGTGLTICRQMAAGGIVMGIYGLWIATGRLPDGLSLATRVVSSLWAVA
                     IFAALGMFTGAALASFGLLAVRPLSQQRRVRIAAALEATIVVPLVLWLVLNEFTYSMT
                     SQVIGLATLRMIWSIPDAIFENAWEMGGRYLIAAGVMTIAAAIVVYRLVARSMLVYRP
                     NTSQHASSAEAALVKRPLRLAGSMSGVLAVVTGLVVVQLCTRPSEALTTVFKSSPPLR
                     AFNVARLVMGTDLLGPVPTTFGPAIMSEAEYQSAMGKPRQPAPNVILILLESVSASAL
                     HCYGYPKADVSPNMDRLAAEGTLFEHCISPASFSTYSVVSIMTSLSMLRASDNDHFGD
                     VSFPFLGLPRTLKLAGYELAIFSSGNEAFDRINHFTPPSDYDTYFTHDTWGQEKADCM
                     RMDDRHAVGHFEQWLAGRSDPRPFYCSFYLQSTHFNYEVPEPWFSYYQPVPPLYSNGD
                     GIIHIPADVVPLLRNQYDNAMRYGDHWVGRIREVLAKAGALDNSVIVIVGDHGEAFMQ
                     HGLARHGVHLWEEMIHVPLIVWVGPALRQAMPYALSSRVSDSVSSLDIAPTIAGLVGI
                     RPHPSWQGVDILAPGYSGRDRPIFSVLQLTQWMECVTLDKWKYIYDLSDLNRLLFDLR
                     TDPGEQINLLTQEPQRAAALHAMLAGWHTYQVSYYARRRFDQYVGRYEPSADLIAAFH
                     NRTPWPRPGPTAPEAVAARQ"
     gene            complement(4038..5858)
                     /locus_tag="P5316_00240"
     CDS             complement(4038..5858)
                     /locus_tag="P5316_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR83407.1"
                     /translation="MTRSCLFLSAAGVLLASASAWAETIDRPLIFSINPVGNREAVGA
                     PRQGSDFVRYDELLFQRISEAGSRSARVLASWREVEAEKGKWDWSSLDREIELCAKYH
                     IEPVVLICNIPAWVSPTGKSAHNHPPKEEHAADFTAFITRMAERYKNRAKYYEFWNEQ
                     NGCSWINEGCDNARMAHTYLPWLQRCFKAIKAVDPVAQVAIGGLDDADGHAPIFVEQC
                     YQLRRDKYDNAKLWDAIAEHPYAKRPTETTDELIHKLDAIRAVAARYGDAGVPIWITE
                     YGWNVAEMGIEVQQHGTAEFLKAFSRSDQKDVLIAQQLAIADFEPVHLGFGLCDLNLR
                     PRPAFQTFQELARPGTPRPVTLRFVLQPDGVPVINGLIASASEQEDPPSLIEVFNEEG
                     KRVISEKGDPNILSVALPGLPPDTPLLAVISSIVGEKQGIPIARAPFIAPKGLIPNGG
                     FESLFRAGIPWAWTVTGPSICRDGGALGKEYCHGGGHALLLAQLPSSSKRAFDDRIEV
                     PVRAAKGGRIRVRWFARYFGPQDQAVAVMMTASLFDPDEERGGRSPGKPIGKEWTEVV
                     LDLSAPCDGPILCLHLASSRLPKDRWLVAVDDVSVEPLSE"
     gene            6044..7327
                     /locus_tag="P5316_00245"
     CDS             6044..7327
                     /locus_tag="P5316_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83408.1"
                     /translation="MRSFAFRMRILLWVAVAIWSQLTGCGPQTVTKWYKGNTHTHTWW
                     SDGDSPPETVVKWYKDHGYHFLVLSDHNVLSEGERWVAADKRRGEAARIYEETFGNAK
                     VVKRQGADGQTEYLCAPLDKVKAQFEEPGQFLLIQGEEISDSAEGKPVHLNGVGITTV
                     IPPQRGSDIFVTLQNNVDAVLAQEAQMCHPMLVHVNHPNFGWALRADQMARLEGSVMF
                     EVANGHPGVNNLGDETYMSTERMWDVVLTKRLAEYGLPIMYGLGTDDAHNFVATGGAG
                     PGRAWVVVRSSALTADCLIEAMYRGDFYASTGVILKDIRFADDRLDIEIEPRPGVGYK
                     TQFIGTLAGYDDTVAYLPSDPKRGPTCRYSDDIGRVLSEQTGTIASYKLTGKEIYVRA
                     KVISTARHDVPVVAGDFQVAWCQPVQPGCRSGGAR"
     gene            complement(7351..9117)
                     /locus_tag="P5316_00250"
     CDS             complement(7351..9117)
                     /locus_tag="P5316_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83409.1"
                     /translation="MKDALGMAICASVLLPLSVNRNAAAEFIDTQPDTWAAVDGLGRA
                     APNHDQVGGPRAGKSVGIFYFLWLGQHRTEGPYDITRILASQPDALGRPTTNPWGPEY
                     HFHHWGEPLFGYYLGDDAWILRKHAQMLSDALVDVAVFDVSNQETYPKVYRKLCEVWM
                     QVRRDGGRTPQIAFFAPFGDDRRVLSRLYDDLYKKGDYADLWFRWKGKPLILARKESA
                     PPEMQETFTFRPHGPDYFSGPTGPNQWQWLQIHPQAPYYNDTNEVEQVSVSVAQNASG
                     KRLCAFSEPNTYGRSWHDGIKDERPDAVLHGLNFAEQWKRALELDPEFIFITGWNEWI
                     AMRLPEFNGVREPVMFVDQFTQEYSRDIEPMKGGHWDNYYYQMVSGIRRFKGVRPAPT
                     ASGPTTIQIDGDFSDWAGVQPGFRDDRGDTMHRDHPGYDDAGRYVNKTGRNDFVLLKV
                     AADDHVVCFYARTASVITPSTDPNWMLLYINADNDLATGWESYDFRVNRRLRGQTKSV
                     LEKAGTNGVWTAIDEVPYRVSGSELELAVPRRILRPRDDSPLRLGFKWADNMQCDNDV
                     MEFTVNGDTAPNARFQYVYAMK"
     gene            9410..10417
                     /locus_tag="P5316_00255"
     CDS             9410..10417
                     /locus_tag="P5316_00255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR83410.1"
                     /translation="MGSRLISIVVPVYDEVDSLDGLVAEIRDVAARNDLEVEVILVDD
                     GSRDASWERIVSLARNDKRIGGLRFRRNCGKAAALMAGFAVARGNLVFMMDADMQDPP
                     EEMPRMLAKLDEGFDLVSGWKRIRHDPWHKVYPSRVFNKMIGWLTGVRLHDHVCGFKV
                     MRREVAKDLRLYGEFHRFIAVFSAARGYRVTEIPTLHRARTRGVGKYGLTRFFKGFLD
                     LLTVTTLTRYRWRPQHVIGVTGVVVVLLGAMLPLLAVIAGQMSSELGRLNEWLGRPFE
                     TIRGLTAMLAWLLLVLTPGLVLIAIGLVAELVVAERPLDRLYEVVEKVGWCAETEPAQ
                     G"
     gene            10592..12565
                     /locus_tag="P5316_00260"
     CDS             10592..12565
                     /locus_tag="P5316_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83411.1"
                     /translation="MPTDVTVVFMILAAMVISFATLTRTKLEHCVNDGSRWNTVFYLV
                     EYGTYEYLPDWGEIKGVSKRGVSDPDEHIARHNSNSTPEDKARIKGLMRKFGDRYYRH
                     VWDIPPFSTVDMISVKDKNGQDHFYSSKPPLLSTCVAGVVMGLEKIVSPIMAMPSVHE
                     YLTGSRPEVRSGAATQPAGTQPSQTPSPGSFTFREHPMFYMRAAVLLVQLVPFLIMIW
                     VIRQRVIEQTDSPFVQNFCVGAAALATFLTSYAAPLNNHIVAAYCAMFALHAMLRVWY
                     DGRREWYWFLIAGLFGGLCHGLELPATLLTLAILVALFWKEPRRALVVGLPAMLIPIA
                     ASLYTTYLVTGSVTPIPMRFDEPNGPFFYDGSYWHPNNYYTPRGVSIDFLQEPKQKYM
                     FHLLVGHHGFFSLTPLFVLSLIGIGRHMAGIRARRAVLASVVLAAIFLAAAILWKADA
                     GGSSLSWPRPDAARVASTHAMAGDVSAASADSADGSRFTRWWADFKLGLACIKTAVSH
                     PVAAIDQAADLLWGRGFLAFIAILLVVNLGMYLGEPDQKHSLLAAGTLFLFLAVLLFY
                     TFRTHNYAGVCQGPRWMFWMIPLWLLMLPAGIGPLAERRVGRWLCYLCLFVSLLSVFW
                     GLPRPDEDVRATDRPFTSSWLHDYMRWRNYIDY"
     gene            12555..13382
                     /locus_tag="P5316_00265"
     CDS             12555..13382
                     /locus_tag="P5316_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HRR83412.1"
                     /translation="MTTDVNQEYEALRRESFGWIKAAARIALERLGRADVTRKHDKSP
                     VTDADHAVQDALLASIAKLYPQDAVITEEIQSRPDVHQPVSRSGRCWVIDPIDGTRNY
                     VRAIPVFTVSVAVMENGQPVVGMVYDPVADRMYSASIGAGAWVNADRIERREVPTWNE
                     VYMGIPTSKHEQLPPVVHRWIDTMVVRNFGSTAMHLSLLATGSLDAVYCKKSKLWDIA
                     AGAVIACETGAEVLSLDGREHFPMDLSHYANEPLPMIAARPDLVERLLAEYRTAQMS"
     gene            complement(13601..15514)
                     /gene="dnaK"
                     /locus_tag="P5316_00270"
     CDS             complement(13601..15514)
                     /gene="dnaK"
                     /locus_tag="P5316_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRR83413.1"
                     /translation="MSKIIGIDLGTTNSVVAIMEGDQPKVLTNAQGSRLTPSVVGFTD
                     KGEQLVGQIAKHQQVTNPENTIFSIKRFMGRRHYEVQSEEKTVPYKIVGGPQDLVKVE
                     VRGKQYTPPEISAIILRDLKKTAEDYLGETVTRAVITVPAYFNDAQRQATKDAGEIAG
                     LKVERIINEPTAAALAYGLEKKKNEKIAVFDLGGGTFDISILDIGDSVFEVLSTNGDT
                     HLGGDDYDKVLIDYVAEEFRRKEGIDLRKDPMALQRLKEACEKAKCELSTVQETTINL
                     PFITADQAGPKHLQEKLTRAKFEQLTQHLTDRCRQPVMTALSDAKLTPKDIDEVVLVG
                     GSTRMPAVQRLVKELFGKEPNKSINPDEVVAVGAAIQGAVLAGEKDDIVLLDVTPLSL
                     GVETLGGVMTKLIEKNTTIPTSKKEIFSTAADGQTEVTIHVLQGERQMAADNRSLGRF
                     NLTGIPPAPRGIPQIEVTFDIDANGILNVSAKDLGTGKAQSIRIEGSSGLSKAEIERM
                     RREAEEHAAEDQSRRELVEVRNQADNMIYQTERTLKEHGDKVPANERGNVEAAINQLK
                     EAMKGDDKAAIQRAMEQVATSSQTIGKIIYEEVARKAGGTAGQAATAGATAGGASQAK
                     ADDVIDAEFEEKK"
     gene            15834..16313
                     /locus_tag="P5316_00275"
     CDS             15834..16313
                     /locus_tag="P5316_00275"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HRR83414.1"
                     /translation="MSLLHKPLETVGGHGAPAVRQISVFVENRLGQLLSITQVLEKKD
                     IKMLALSIVDLVDCAIVRLLFDAADEAIRVLREAGFTVTVAEVVVVRLPKGKRGLLTV
                     WSALLGAEINIAYAYPLLASQKGSAIAIYVDNVDLAIDTLRTHNFEVLDESDLQEET"
     gene            16493..17194
                     /locus_tag="P5316_00280"
     CDS             16493..17194
                     /locus_tag="P5316_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRR83415.1"
                     /translation="MLGWWDHQYFLLVGPGILLALYASLKVKSTFARAKEMTTRKGWT
                     GRDIAQAILDSEGIHDVAIEPVNSYLGDHYDPRERVLRLSPDVYHGRSIAAAGVAAHE
                     AGHAIQHAHGYAALSLRNSIIPLASIGSNFSFILIMIGLAMALKSLAVIGCALFTMVV
                     LFQLINLPVEFNASTRARQALLANGLVTQVEDEEVGRVLRAAAMTYVAATLTAILTLL
                     YYLMRAGLLGGHRDD"
     gene            complement(17220..17606)
                     /locus_tag="P5316_00285"
     CDS             complement(17220..17606)
                     /locus_tag="P5316_00285"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR83416.1"
                     /translation="MRAREMVIGEVPKLTRDVIAEIVRQLPHEAPHQQDDRNDPRRHP
                     RYSSRGTVELRPVFSRQSDSLTGNVMNISEGGLGMSSAHYFEPDSVVDIVVQLPHASF
                     ATKACVRYCKKVRGEYMTGLAFIFGS"
     gene            complement(17679..18053)
                     /locus_tag="P5316_00290"
     CDS             complement(17679..18053)
                     /locus_tag="P5316_00290"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR83417.1"
                     /translation="MPTGEIVKLTRKQIARLIRKRMETAGQRYDGAELRRAPRWPFPG
                     TVELRYATGDNNQQWFAVCRDISEGGMGVKTDTYFPPDALLDISVHLPEQTFYGQAKV
                     RYCKEMDGEAEYLMGIQFVFDD"
     gene            complement(18119..18859)
                     /locus_tag="P5316_00295"
     CDS             complement(18119..18859)
                     /locus_tag="P5316_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83418.1"
                     /translation="MRYLQWTFGLAVLGAAIAGTLWMLSPGETARLRRENEELEKRRI
                     ALEQAVSRLTGEDRVAEVYVVDQVREGEVFNGRPSDTDITIIEFVEIDREGHALPSRR
                     FMINDDVIFFDALVLKFDPEYVSLGDALRGKSLALFRRIYGEHQNPIDGFPVDPSGDV
                     PNIFRVNPSPTPFEQELWRQFWDLASDPDLAARKGITVAQGEAVYVPVERGQVWSLTL
                     QNNGGLSIKLRRSNADQTSRPSLSPSVR"
     gene            complement(18977..20482)
                     /locus_tag="P5316_00300"
     CDS             complement(18977..20482)
                     /locus_tag="P5316_00300"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRR83419.1"
                     /translation="MNTVNLPIALRSLGRLALVLGLGLAAAGVPSTLMAAAPVAAAGS
                     AKAEPGETITITLSATDADSDPLTYIISTLPTKGSLAVGSTTLAAGDLPYAIPAAGRT
                     VTYTATTTAHGTDTFTFKANDGTQNSTAATITVTVNREPLTGMTELTTPPNTDLNITL
                     PVSDADGDTLSFVIVSLPGHARLKSGSAILTDADIPYATSSNKLTYSPDTDYHGLDTF
                     EFTASDGYVTTGSISVAVQINTAPVPEDQRVTLLPDSSVTIRLIANDADRDPIEYTIA
                     SLPGHGTLSTGEVLIEEGDLPKTLTRGVNEVLYSVTPGYRGTDSFHFRVSDGVAGSDR
                     AVVAVAVNTPPLSPDSAVSGFCGTIITGVLSPSDEDGDKVTLRLTALPKTGVLKINDV
                     TASLTATYTAASIGPPFTFSFTPDAGAVGTETFQWCANDGREDSIPGTVTLAVLPLPD
                     GGGPSDGGSDGGSSSGSGTGCGALGMGEVVFLTVGLLLVPSRTRRLPFSHP"
     gene            complement(20712..21581)
                     /locus_tag="P5316_00305"
     CDS             complement(20712..21581)
                     /locus_tag="P5316_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83420.1"
                     /translation="MRATSRSMLDHTFALIVLLLPAAVLAQPDPAVPPEFDLYAGRVW
                     SKDLIAGSEISQQFTPASSLPEAQRPPLSGLFDSFGMYMESNGSFVMYVSLYQWNISW
                     MRTVLIDGPIKTWRVERTAGGPGWVMLDLDTITEDERSCISYPALAPLAVDGSYLWDL
                     EVTEAPAPLRCWSTGVKGDLGEPSGADNQGFHDDAAAKDWAFQTRLHLVSRCSDPRFD
                     VDGDFDVDQVDFSAFQRCYTGTSPGNWAYDDPCKCLDVDEDNYIDQADHLVFERCASG
                     PGIPPDETCDESP"
     gene            complement(21635..22642)
                     /locus_tag="P5316_00310"
     CDS             complement(21635..22642)
                     /locus_tag="P5316_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83421.1"
                     /translation="MIAMRESNLGLATTLMVVFSLSGLAAGAPGPPDPPDYDLYAATV
                     WEKAWGAGQEAGQQFSPASSLPEAQRPPVIGGFDSFGVYINSTGDYEFYMEFYTWDTN
                     WSTTIANGPMKVWRVVKVGQTGEAWIMFDLGSESVDLRYCNDAGTGPKVYPALDPLPL
                     DGSYFWRLLVTRASGSPLQCWATTRTPVVDCGVGNIGIHGGGAVRTWSYQTRLHTLTP
                     TCFNPRYDTDNDTDVDRDDFTVLQSCYTGAGIPGTWPYDDPCRCLDIDGDDDIDSVEY
                     LAFDACALGSGVPADPACDDPQACCFADSSCQNLASVVCSDRGGTPQGSQTQCNTVAC
                     P"
     gene            complement(22785..23495)
                     /locus_tag="P5316_00315"
     CDS             complement(22785..23495)
                     /locus_tag="P5316_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR83422.1"
                     /translation="MRGKHITVLACLATIGMTVFSSPVLAIRITDPGGLDAPPPWYVL
                     YDQTGTQARNTGAADWFSSRTDPVNGDNRLDKPPNGCTFTKIAVYVMPGSGTITDLNL
                     YLYRTVEGVDAITTWNNTVQPANLIASQSFGNGQPAAGWLVLEFPENPQPWPAASPMD
                     SGYVWRLVWTGGGNIQFAKWHRPGNRDTTIVGNTQFDAGAFPGQENRAFVTAVYVPEP
                     AALALLGFSGCMAMRRRR"
     gene            complement(24068..25303)
                     /gene="metK"
                     /locus_tag="P5316_00320"
     CDS             complement(24068..25303)
                     /gene="metK"
                     /locus_tag="P5316_00320"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRR83423.1"
                     /translation="MVTPTDKRRYLFTSESVSQGHPDKICDQISDAVLDSLLRHDPMA
                     RVALETMVTTGLIVMAGEITVHNTAAERALAEAETTARETVREIGYTDSAIGFDYRSC
                     AVLRAIHSQSPDISQGVTKGKGRHKEQGAGDQGLMFGFACRETKALMPLPIHLAHRLV
                     ERLTEARENGEIKWLRPDGKSQVTIAYDGGVPTRIDTVVISTQHTEEVVDKSGNLSRQ
                     AQAKIIENIIKPVIMRERPDLWSNRIRYHINPTGRFVVGGPHGDSGLTGRKIIVDTYG
                     GRGRHGGGAFSGKDPSKVDRSAAYMARYIAKNIVAAGLADECEVQFAYAIGVAEPISL
                     LVSTNGTNRVPETTLERLVRDMFPMKPAEIIQHLKLRRPIYRETARNGHFGRTGPGFT
                     WEKTDMAEKLKKAAAKWLK"
     gene            complement(25480..25911)
                     /locus_tag="P5316_00325"
     CDS             complement(25480..25911)
                     /locus_tag="P5316_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83424.1"
                     /translation="MPQTDLNHLILIVTGASLRGEAADRPLAYQLAREIAERLGPESP
                     WKPLVISDVLYINEKRLSKQPVISVGGPGVNHLSALLFQELPSVVTIDNVLIIQMDLE
                     LTDLRCCLWGMNHEQTVEAMNLFLKRGYLDRFLEGLTQKSR"
     gene            complement(26057..27196)
                     /locus_tag="P5316_00330"
     CDS             complement(26057..27196)
                     /locus_tag="P5316_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRR83425.1"
                     /translation="MNKETLSRRGILKTAAQSGAAVCAVAAGSARLAAQAQTGGRDPA
                     QGDQRLSLEQLRQWESLRYGMFIHFGMSTYVENELPDGKAPASVYAPDRLNVDQWVSV
                     ARDAGMKYAVLTTKHVAGHCLWPSKHTSYTVANSGNKTDVVEQFVKACEKRGVLPGFY
                     YCSWDNHNRFGSRTPSDPNTLWEFGMSRFAELERRTSTQPSAPLPAYTTSVYQGFQTM
                     QITELLTQYGPIAETWIDIPGVLGRGYREYLYNTIARLQPQTVIMMNSGISSGESYPV
                     DYAWPSDIIAIERRLPPGKGHEKWRTIEGRRCYMPGEVCDPIGKDWFFTPDDKPRSDD
                     ALLDILQSCLDRGVNLLLDVPPDKHGLIPDMHVQALQRLRANAKI"
     gene            complement(27450..29003)
                     /locus_tag="P5316_00335"
     CDS             complement(27450..29003)
                     /locus_tag="P5316_00335"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR83426.1"
                     /translation="MRRTRHLAWSIAGMVAFLLVGCQQPSGTDSASAVRHETRVRAEN
                     ALWIETPPERRFHNNREVVVAEMQGPGMITMIHFAYPHRAIAAPKEYHLNRDLLLRIW
                     WDDEPEPSVDCPMVDFFCDPAGLREVVNTALTNKKRGWNAYFQMPFRKSAKVMLVYEG
                     PEPPGEKLWAMMPCYSYVMWRSTPVPPQAPYFHAWWNQKAVLTGKEDYPALRAQGRGE
                     FIGWNVTVRRPGTSGYPVDMNEKFYVDGEETPSVEFQGIEDSFGFSWGFPEVENIYPT
                     LGYWPFMKGAAAYRFFLNDSIAFRKSLAVDIGYGVNEDPMFRREFSKPGTQLQFSSTC
                     YWYQTEPHLPFPPMIPAAERVPAPEKMFWPEEPPPLPSAEDLRARGVKLYMACGYPEK
                     EILFAEPGFGAAVKQGATWTGWPPPVFHCRSDDHEIQFELTVPQDAEGTLRLYLIDPD
                     SFEGGRKQEVFIGDKSLGTFEKFQDGRWIDAPVTRDMTADGRLLVRVANRAGKGNAVI
                     SMIEWVSPK"
     gene            29233..29904
                     /locus_tag="P5316_00340"
     CDS             29233..29904
                     /locus_tag="P5316_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRR83427.1"
                     /translation="MADQPTMTPQMQTSYNTSPKVGMAVAALVCGILGFCFPPLGLVG
                     LILGIIAVIRASSDPVRHGGKGMAIGGICTGGVSLVFMVFIGPMMAAILLPSFARARE
                     LAKRTACQANMRPLSMAFLNYTVENNGAFPDDPAKALARAGISAKNLECPSSGGQSNY
                     RYVPGWSTKNGANKPLVYEPIANHGDEGGNVLFLDGHAEWVEKTRWAKVVGPYEDKSV
                     PISPP"
     gene            29950..30996
                     /locus_tag="P5316_00345"
     CDS             29950..30996
                     /locus_tag="P5316_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRR83428.1"
                     /translation="MTVAGAKRSDHLLKVLSGYQHVLVLTHDNPDPDAIASGWAVAAL
                     VRAKAALPVRFVAGGAIFRAENRRMVELLEPPLEFVEQIEVPEGTATVLVDAEPHAVN
                     HLLGEAGLPTAVIDHHQHEGRRPRLPFCDIRPKVVASATIAAHYLREQHIDPGPALAT
                     ALTYAIRTDATGLRGFSAADHRAMRWLSLRADHQKLTDIENAPLQPDYYADLLLAMQN
                     TFLYEDVALCFLPRAGGSEIIAEVADLLIRHRDLTRVMVGGVVNGDLLVSVRSKVGAG
                     DASDLVARSLEGLGHGGGHCQRAGGKVPGMARGERVSEDLQSMLRNRWLGVCGVDAQR
                     GMRLVPKREILENL"
     gene            complement(31223..33394)
                     /gene="pulA"
                     /locus_tag="P5316_00350"
     CDS             complement(31223..33394)
                     /gene="pulA"
                     /locus_tag="P5316_00350"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HRR83429.1"
                     /translation="MRLTTRLPRTKRMCPEMVLATLDAECLIGLRFRRRMRVAECRPE
                     RFRLIGDDGRVIGIRSTQARRPLRGRSSRYLLETERPVDFRGCSWVIHSEVFGRTPLR
                     MGGLLQDPALFYDPSTVLGASWMPEATTFRVFAPTAAAVRVVVADSPSGDHNRRLHDM
                     QPCSYGIWEVTIPGNLRNKYYAYVLEGPGFDPAREITDIYATCTQGRSPRSLIVDLLE
                     TDPPGFRNHESPELAHPADAVIYEMHVRDFTIAAGSGVRYKGKYLGLTECGTHLPDDP
                     TVCTGLDHLCELGVTHVQLMPVADFDNDETDADPYDWGYMPSYFNSPDGWYATSRQGT
                     ARIIELKRAIQAFHERGIGVILDVVYNHTSPCASFERLVPDYYYRMTCHGRFSNGSGC
                     GNEFRSESPMARRFLLDSLKYWVREYRVDGFRFDLMGLIDRKTMLRIKTELEAIRPGI
                     LIYGEPWAAGPTPLRPLTDRWRLQNTGIGAFNDGIRNAIKGDVNGTEPGFIQTGEHIE
                     DLRHALEGSIHLNTVNPSETINYFECHDNLTTWDKLALSVPHETDETRRRMMRLAALI
                     LFCSQGIAFMHSGQEFCRTKRGSHNSYNLPDEINRTDWSLKKTYADVWAYHRGMIAIR
                     RRHPVLRLPTRQAIEQRVSFNEPPHPKCVVCHLDGSGLKGESAEEMLILLNGHHEPVE
                     FRLPSGKWSVLADAARAGAEPFGTAGERATLPPHSGMLLSL"
     gene            33619..35067
                     /locus_tag="P5316_00355"
     CDS             33619..35067
                     /locus_tag="P5316_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR83430.1"
                     /translation="MPIEQAELVDRLRWFVQLRWIFGVVALAAATLLLRIPLGRVHAP
                     MLVGISVAILCYNWLFWFLEGWLSRHRPEAHARYAEVAASLQIVLDLVALTFVLHASG
                     GVGNPFCLFYVFHMVIATLLLRMRHVFLLAGLAIFLFTSLALLEMRGWVPHHSLFGLE
                     EAYKSPRYVAITLVAFASAMLTAVYIGTSIAGRLREREHEILRLERQLADYARELERT
                     NQELVRADEEKTRYFRKVSHDLKSPLAAQQSLLRALLIQMEKSDPQSAARVERAIARG
                     DELLALLNDLLLLSQAKDVVRGPRHEWIDPGDRLRAVIDAQALAAKEKGLDFFEEMET
                     PLPAICAEPGTLLTLAENLLSNAIKYTPKGGSVTFSLFSRDEHLIMVVKDTGIGVAKE
                     DLPKIGRQFFRTSQARESGSPGTGLGLAIVRSMVEAMHGRFDFQSELGAGTTVTVALP
                     LSHPEALREVEPFCRLEERRADEGTVASRERV"
     gene            35107..37245
                     /locus_tag="P5316_00360"
     CDS             35107..37245
                     /locus_tag="P5316_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRR83431.1"
                     /translation="MKTVRLTMLDFREPAARWGIMFLFSAVVGVLAGLAAAGLDYGLR
                     EGTRLLIGRFTHIGGAQVFAFRWGILLMPAVGALVSSLFVLLLCRKDSGHYGTDQVIA
                     AFHQHGGHLSLWAPVVKALAAIGVISCGGSAGPEGPIAVLGAGIGSKFARLLSLAPRS
                     RRILLIAGCGGGVGAIFQCPLGGALFAVSVLYREPEFEAECIVPAFVSSVLSYSTFMY
                     FPGFGHHLLQGVNSLGFASPAELLAYAVLGLICGLLAGFFYICLRMVESRRLLSARLS
                     AWLLPTVGGLLVGLTACVLPQIMDSRYQVLQNTLDGKIFLGDSVAGRSAWEWVAFFGF
                     LAMLKCLATALTVGSGSAGGLLGPSVFIGGAAGAFLGALLEACWPGHFPPGSPLRQAL
                     VPVGMAGVLSASMRIPLAATVMVTEMTGSYGLIVPLMVTCVTSYVLGRRWGLNPSQVR
                     WSAESPAHAGDLLGRMLESLQVQHLMDRHWRYTVTPSTTLGEMIRQIGSGSRPVFAVR
                     EDGRFVGVISVQDISRAISEPGISEIVIAADIMSGHDTTVTPEQSIAEVLEVFQKTDL
                     DVLPVISDDSERRHLGMLNRRAIHEAVRSRLERIREHARREHAGIAAIEQDEQLFQLM
                     LGVSGSRPDVFTRMPVPNEIVGVSIRQAAFQRRYGAQVVAIQNQDGSLQCPPDIDAPL
                     REDQILVVIVSDVRQAEHGTVAGPEMSSHL"
     gene            37502..38314
                     /locus_tag="P5316_00365"
     CDS             37502..38314
                     /locus_tag="P5316_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRR83432.1"
                     /translation="MTGDERRQQLSEVLARRGYADLSLLVSELGVSESTVRRDLSLLE
                     EEGIVRRTHGGAVFVSDRFVALNYAAREVTAVPEKEAIGRVAAGLLQDGQTILLDGGT
                     TTFQVARNLLSRTMQVVTNSLPIANLLHSAPNIELIFIGGYIYPRTGVALGPYAQQML
                     SSLHVDAAVMGVAGITEDALYNANVLMVEAELQMMRSAGEVILVADSGKFGKRALVRL
                     GGWEMVDRVVCDSALEGRWQDAVRNAGAELILAGVEEATGVGARSAGAGGQG"
     gene            38311..38997
                     /locus_tag="P5316_00370"
     CDS             38311..38997
                     /locus_tag="P5316_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HRR83433.1"
                     /translation="MSTAMVGGRLDRSVVEGVVRDIVRQRLGLGPGRAGAAKPKLVVN
                     ISARHMHVTQKTLEILFGPGAQLTVMRPLYQEGYFASEQTVTIIGPRHRTITNLRILG
                     PVRPEDQIELAFTDGIAMGLDLPVRMSGDIKGTPGCIVLGPKGHIELKEGVIRAQRHV
                     HMHPTDAEYYGVKTGDFMRLRVHGSCPTTFEGIICRVSKDVKLEVHLDTDEGNACDLA
                     NAARVELERM"
     gene            39053..39343
                     /locus_tag="P5316_00375"
     CDS             39053..39343
                     /locus_tag="P5316_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRR83434.1"
                     /translation="MALEALGMIETKGLVPLIEASDAMMKAANVRLVGYEKVGRGLVT
                     AFIRGDVAAVKSATDAGSAAASKVGEVVSVHVIPRPHDELAGMLPAERNRAK"
     gene            39427..39696
                     /locus_tag="P5316_00380"
     CDS             39427..39696
                     /locus_tag="P5316_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRR83435.1"
                     /translation="MSDAIGMVETMGYTGLVEASDAMCKAANVELVKSVQIGGGLVTT
                     IVRGDVGSVKAAVDAGAEAANRVGELMSAHVIARPHEALADVYFK"
     gene            39756..40910
                     /locus_tag="P5316_00385"
     CDS             39756..40910
                     /locus_tag="P5316_00385"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRR83436.1"
                     /translation="MIVLVANIGSTSFKFRLFDMDGERELARGGAERIGSDNAAVKVG
                     DKTWAGRIADHGQAIDHCLRLLPDPNVKLDAIGFKAVHGGTTSGAVRVTEEVIRTMTE
                     FFDIAPAHNPPYVAAMKAFAARLPDVPQVAAFETAFHQTIPPSRTTYGVPWEWTQAGV
                     RRYGFHGASHRYIAVRTAELMGRKDLRVISCHVGGSSSICAIENGRSVANSFATTPQS
                     GLIHNNRVGDFDPYALLKVLPAFKLTFEQALQEMAKKGGLLGISGVSNDRRDIEQAAA
                     EGNERARLALDVLVEGVRHYVGAYLAVLNGADLIVFTGGIGENSASFREAVCARMDYA
                     GIKLDSRKNETRGREETISADDSRVRIMVIPTNEELIVARQTRDVLAGGN"
     gene            40971..41246
                     /locus_tag="P5316_00390"
     CDS             40971..41246
                     /locus_tag="P5316_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRR83437.1"
                     /translation="MAQQALGLIETRGMVALVEATDAALKAANVTYRGWKQVGSGLVT
                     VVLEGDVAAVKAAVDAGAASAAKVGEVVSVDVIARPHGDIGKILPNK"
     gene            41384..41695
                     /locus_tag="P5316_00395"
     CDS             41384..41695
                     /locus_tag="P5316_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRR83438.1"
                     /translation="MFIGKVTGTVVASQKVKSVIGRKLLLVQAMNVKADAPGALETTG
                     RTAVAIDTVGAGEGELVLVTQGSSARLTETTGDVPTDAVIIGIIDAIQMKDQEIYKKQ
                     G"
     gene            41777..43210
                     /locus_tag="P5316_00400"
     CDS             41777..43210
                     /locus_tag="P5316_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HRR83439.1"
                     /translation="MSTTVSEALIRDVVSEVLSRLQGNGRVATAPPVPAGGRDGIFQT
                     VDEAVAAAKVAQKRLVAETLETRAKAIKCIRRICLEQKEELARIEYDETRIGRMDHKI
                     EKLIVVGEKVPGMEMLTSEAVSGDHGLTVTEHAPWGVLGVITPVTHSIPTLGCNAIMM
                     IAAGNTLVCNPHPSGARCAAVATQRWNRAIREATGLDNLICLIEKPTLQTADQIFKHP
                     DIAMLVVTGGPGVVAAAMASNKKAIVAGPGNPPVVVDCTADIEKAAAGIIFGHAYDNN
                     LLCIGEKEVIVVEAVADKLLEAFSRHGGYRLNAEQIRAFTAMALHKDEKSGHWLPDKK
                     FVGKDPQVLADAIGIRLPQGTQCLYGEVDEHSPWLPCEQMMPFLPVVRAKSIDQAIAM
                     AVEYEHGFGHTAIIWSRNVENMTKMGKAVDTTIFVKNGACMASLGAGGEGFASFSIAT
                     PTGEGITSPMTFTRYRRCVMVDNLRIL"
     gene            43221..43499
                     /locus_tag="P5316_00405"
     CDS             43221..43499
                     /locus_tag="P5316_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRR83440.1"
                     /translation="MILGRVVGNAVSTVKHKSLGGARMLVVEPIQAASLDPVLAIDQL
                     GAGAGDIVVLTSDGIYTREVLNDKTSPARWTVMCIADDGQYVMEHLEE"
     gene            43496..44101
                     /locus_tag="P5316_00410"
     CDS             43496..44101
                     /locus_tag="P5316_00410"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRR83441.1"
                     /translation="MSRPVVTAILLEDLRREGKEIRLPEGALVTPAARDWLKEHSLPV
                     TWEKAPAGAPGRLAVVMDPSLSELRMMRAMLDRGGQLAEVIEPAGGRSGMLAATRRLC
                     GKIARGEVAKGVVFAADGPVPVCVANKHNGVRAALGVNIPMTEEACRSLGINVLVLEY
                     PTLTPYQMKQMIDRLLAGASPPAEMLAEIEAVEQGGGRADR"
     gene            44088..44372
                     /locus_tag="P5316_00415"
     CDS             44088..44372
                     /locus_tag="P5316_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRR83442.1"
                     /translation="MRIGDVVGRLTLSRCDPKMIGGRFLIVQPHDPKSLRENRPGKGE
                     VVVVYDQLGGRVGDRVGFTEGREAAMPFYPDRVALDAYLVCLLDCVTYTA"
     gene            44397..45245
                     /locus_tag="P5316_00420"
     CDS             44397..45245
                     /locus_tag="P5316_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRR83443.1"
                     /translation="MVNEFKLKQEICEIGHKLYEKGFVAANDGNISYRVSENHVLCTP
                     TLVSKGALKPDDICLVDMTGKQLAGKKKRTSEVMLHLEIYKANPKVKAVVHSHPPYAT
                     AFSVAGEEIPTCILPEVEVFLGPIPTAVYETPGAQAFARTILPFVNTAKIVVLKNHGT
                     VSWGETLQQAYWWTEILDAYCRILLIAKQVGRVERISPPKVEELLDLKERFGITDDPR
                     RLYNADLCVNIDFGRGYSEPGSTLMKKPEGPMRPPEPVQAPAVSNEEVERVVQEITDR
                     IMSALG"
     gene            45276..45878
                     /locus_tag="P5316_00425"
     CDS             45276..45878
                     /locus_tag="P5316_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83444.1"
                     /translation="MDADVCHARIKGMFAVAVAWLFVLCGCHQTRYNWVLHDVDVTLP
                     EMTAAGRVTERAGTPAELLVVRPGGGKTMLVTALITDDPEKIDDEKAETFVCVLDGPP
                     LIGRKTAVDVDRCRLILNEVFRPCRRPYQGAEGSVRITSVRGGKVTAEIVFRNVLRSA
                     RDDSYIVRATKTFRPAAPDDVRLRQAGIQYEPTPAPGTLP"
     gene            45955..47181
                     /locus_tag="P5316_00430"
     CDS             45955..47181
                     /locus_tag="P5316_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83445.1"
                     /translation="MVAKSLDIKLARIKADPGCKEFILADAKDADMAFGIAAPGKSPE
                     HYSQEGKFRSLPEFRECIRQIVRQGLVDIMLMSAHTNSILTIHERLFDNSHVTPAARA
                     NDTTDIHLARGSIYASEPSRPFRTVTIDQIQCGKVECQPTERVIGANLGLYSVTFNND
                     LELDRLTLEQYREFRIEAERKGFRHFLEVFDPNAPTNPVPPDKLGGFVNDMIVRCLAG
                     VPEGGRPLFLKMVYHGPKWTEELASYDPQLVIGILGGSAGTTYDAFKMIAEAQKHGAR
                     VALYGRKINNAENQLAFIEMLRRIVDSQISPEEAVRAYHGVLQGLGIKPYRSLEDDMK
                     LTSQVMSYAGGGRVQVPASPAASPPRKPSAAARPASAKPVTKPAQGYVAADAPDFSKM
                     TAKERAAYHRARIAGI"
     gene            47254..47475
                     /locus_tag="P5316_00435"
     CDS             47254..47475
                     /locus_tag="P5316_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83446.1"
                     /translation="MRYLNSTLYSLAAPLAALPLPLMRMQFDNIGDYIRNMEPQLRQI
                     IAEVIVAQISTIAEALIVALTRIFFGIGG"
     gene            complement(47851..48954)
                     /locus_tag="P5316_00440"
     CDS             complement(47851..48954)
                     /locus_tag="P5316_00440"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="App1 family protein"
                     /protein_id="HRR83447.1"
                     /translation="MAQSGIAPDEEVIFFNTAARLDTVAAQWVVPIHGWIFEPETDSI
                     RRMVVLAALRAALDVDDEHAGERLFNRRAAAFLVDNESGKTLTLRIGDRTATVGPSEQ
                     NGHLTGSVRLSLEEADRLSRPDKYGRQWVEFDAVTPKSDKRRFSARAVLAPPAGLSVI
                     SDIDDTVKITQVSDLEEVIENTFLKPYRPVPGMAKLYQRWAQQGAIIHFISASPWQLY
                     APLTEFTNEHGFPDVTFHLKTFYWKDSRFLSLFADPLEYKLGVVEPVIESFPQRQFIL
                     VGDSGEKDPEAYGTLARKYPDRVVFILIRAIENESPDAVRFQTAFEGVPRQRWHVFVD
                     ADELANVALPGIATTRPATASHGAPATTDKAKE"
     gene            complement(49233..49835)
                     /locus_tag="P5316_00445"
     CDS             complement(49233..49835)
                     /locus_tag="P5316_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83448.1"
                     /translation="MPRQGSLWRCVNDEQVYLCPTDLKGMPDAENPAGGGGNGRFSYT
                     MNGILGFKAVEQIQESTYVTDFQVMEGAMITPIQMIPVGTRITWSPAVMTMLIEEHPW
                     NNTNHGFVGDSRAADSYLALRHDPRTTGAVANFGYLDGHAESKRYPYYGNKKGLAGNV
                     FKLQGVDLFNELRFPYTYAGNNGGWENEYAFIHRFRHPYQ"
     gene            complement(49854..50261)
                     /locus_tag="P5316_00450"
     CDS             complement(49854..50261)
                     /locus_tag="P5316_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR83449.1"
                     /translation="MRTALSTPVPGSCETMMGASRRRAASTLIEVLVVVAIIALLMAI
                     LVPSLIRARRLAQGTVCRTHIKQLATAVHVYAAANGRLPGTNTVWGVSYQASSRPPNP
                     AGRRWPNWRSGDSWLGLLCPPDHAWDVNEQQAL"
     gene            complement(50852..51112)
                     /locus_tag="P5316_00455"
     CDS             complement(50852..51112)
                     /locus_tag="P5316_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83450.1"
                     /translation="MAERVHCIEHIICLLFSKVADERGVELPKSCNWPSLTDRTFSDA
                     ITAVRRWLWTDWVFAAHGNHRVFSKLSRPIKAMLMYALAPAA"
BASE COUNT         9547 a        15126 c        16496 g        10410 t
ORIGIN      
        1 attccggctc gccacaccgc agcccctcgg ccaacgcggg ccatcgatga gtcattggta
       61 tctgggcatt ggtcattcgg atttcattgg tcattcgggt ttcgacattg gtcatcagga
      121 tttcgacttt cgtcgttcgg atttcggcat tcccctcccc cctctgaaat ctcaaatcct
      181 caaatctcaa atccccccaa ccccgttccc cgttgtcagc ggcggcgtga aaatgcagaa
      241 atctcgccgt gcgggttgaa ttcggccacc tgcgggccgg ttcggctgtg ccgtgcggtt
      301 ctctgaccgc ttcttacctc aatgccctag actcttgtgc tgagggcact tggggcaaga
      361 aacggtggtt gccaacagcc aagaggcggc ctcgtcgaac ccgcccatga ttggccggtt
      421 ttgcccgccc gctgacagcg gagaatcagc cgtccagaga atggggtgag ggttggggca
      481 ggctgcttta ctggccgggg cggagaggcg cagcaggccc cggtgagcga cggcaaggct
      541 gggttctccc gggcaggtcc ggctgaagaa gacaggcgtg tgcccggaga gaagagaacc
      601 caagtgaaga ggacaggcgc gtgctcggag gagagaagac ccggctgaag aagaccacgt
      661 atttcaaacc ggccagtcta tcggagtctc agaatgtcgc tgaaagcaga tgatgctcgg
      721 cgggcccgca gcgtacagcg gcaactcgga tatcgtccct tgccgggtgc ggttcagcgg
      781 tgtgaggtga cggtctgttg agcaagccag gctgccagtt gggcgggacg gtcggtgatg
      841 atgccgtcaa tgccgaagcc aagaagcctt tgccagtcgg ccggctcatt ggttgtccag
      901 gcccagactc tgtagcctcg accgtgcagg tcgcaaacca gtggggcggt caggtcggtg
      961 tgtttccagc ccacgatccg gacgccggcg gcagcgatgc cggcaagtcg ttcctcggtc
     1021 agttctttgt caccaagagc cccgagcacc tggcgaggct ccagttggtg catctgcttt
     1081 agaaactccc agtcgaagct ctgcacgacc agcttgccga cgagccgttt ctgtcgtagc
     1141 aagcggacat agtcttcggc cggaccgtcc ttgtgttcga gaagcgtcag cgagccggcc
     1201 tggatcacat cgagggcctc ggtcagcttc ggcaggcgct cgccggcaaa ctgggcgtca
     1261 aaccatcggc ccgcatcgag cttgtcgagc gcggcgtccg gtatctcgcg aatcttgatc
     1321 ttctcccgct tgaacacctc cttggcgtcg gtggtgcggt caagcgtcgc gtcgtgcaga
     1381 acgtacagtg ttccgtccgc cgaggggcgg gcgtccagtt cgacgtagat cgctcctgcc
     1441 gctacggcgc tgcgaaacgc ggaaagcgtg ttttccggtg caacagccga attgccccga
     1501 tgagcgatga tcaggacatt gtcaggcgac cctaacggga gccggctcgt acaagatgca
     1561 cacatgatca gacccatagc cgctattgaa ggcaaaaccg ttctcatgac gcgggagtct
     1621 accagaagtc gcatcgagtc acaaacgatg accaggcttg tcgtgaagcg gcgcgaagac
     1681 cgtcgcacgg cttctattgg cgagcggcga cggcttcggg agccgttggg ccgggccgcg
     1741 gccacggggt gcgattgtgg aaggcggcga tcaggtctgc cgacggttcg taccggccga
     1801 cgtattggtc gaatcggcga cgagcgtagt aggagacctg gtacgtatgc caaccggcaa
     1861 gcatcgcgtg cagggcggcg gcccgctgcg gctcctgggt gagcagattg atctgctcgc
     1921 ccgggtcggt cctcagatcg aacaggagcc ggttcaaatc ggacaagtcg tagatgtatt
     1981 tccatttgtc cagcgtcacg cattccatcc attgcgtgag ctgcagtacg gagaagatcg
     2041 gccggtcgcg cccgctgtag cctggtgcga ggatgtccac accttgccag ctcggatggg
     2101 gccgaattcc caccaggccc gcgatggtcg gggcgatatc cagactggag acgctgtcgg
     2161 acacgcggga cgacaaagcg tacggcatcg cctgtcgtag agcagggcca acccatacga
     2221 tcagcggcac gtggatcatc tcctcccaca gatgcacccc atgccgggcc aggccgtgct
     2281 gcatgaacgc ctcaccgtgg tcgccgacga tcacgatcac gctattgtcc agtgccccgg
     2341 ccttggccag cacctcccgg atgcggccga cccaatggtc gccatatcgc atcgcgttgt
     2401 cgtattggtt ccggagaagg ggaacgacgt cggccggaat gtggatgata ccgtcgccgt
     2461 tcgagtacag cggcggcacc ggctggtagt acgagaacca gggctcgggc acctcataat
     2521 tgaagtgcgt cgattgcaga taaaagctgc agtagaacgg ccgcgggtcg ctgcggcctg
     2581 caagccattg ctcgaaatgc ccaaccgcat gccggtcatc catgcgcatg cagtcggctt
     2641 tctcctggcc ccaggtatcg tgcgtgaagt aggtgtcata gtccgaaggc ggcgtgaagt
     2701 ggttgatgcg atcgaacgcc tcgttgccgc tgctgaagat cgccagttcg tatccggcaa
     2761 gcttcagcgt tcgcggcaat cccaggaacg ggaatgacac atcgccaaag tggtcgttat
     2821 ccgaggcccg cagcatcgac agcgacgtca tgatcgaaac cacgctgtag gtcgagaacg
     2881 acgccggcga gatacagtgt tcgaaaagcg tgccttcagc agccaacctg tccatgttgg
     2941 ggctgacgtc ggccttggga tacccataac agtgcaaggc cgaggcggac accgattcca
     3001 gcaagatcag gatcacgttc ggcgcgggtt ggcgcggctt gcccatcgcg gattggtatt
     3061 ccgcttcgct catgatggcc gggccgaagg tggtcggaac aggccccagc agatccgtgc
     3121 ccatgaccag cctcgccacg ttgaaggccc gcagcggcgg cgacgacttg aagacggtcg
     3181 tcagggcctc gctgggccgg gtgcaaagtt gcaccacgac caggcctgta accactgcca
     3241 aaacgcctga catcgacccc gccagccgca ggggccgctt gaccagcgcg gcctctgccg
     3301 acgacgcatg ctgagacgtg ttcggccgat aaacaagcat ggatcgtgcg accaggcgat
     3361 agacgacgat cgcggccgca atggtcatca caccggcggc gatcagatac cgcccgccca
     3421 tttcccacgc attctcgaag atcgcgtccg gaatcgacca gatcattcgc agagtagcca
     3481 acccgatgac ctgagaggtc atcgagtagg tgaactcatt gagaacgagc cagagcacga
     3541 ggggaacaac gatcgttgcc tccaaagccg ccgcgatgcg aactcgccgc tgctggctga
     3601 gcggccgaac tgccagcagg ccgaacgagg caagcgccgc tcccgtgaac atgcccagcg
     3661 ccgcgaagat cgccacggcc cataaagagg aaaccacgcg agtagcgagg gaaagcccgt
     3721 cgggcagtcg accggtggcg atccacaggc cgtagattcc catgacgatg ccgccggcag
     3781 ccatttgccg acaaatagtc aacccggtgc cggcgctggc aaagctgccg gaatgtgtgt
     3841 gaaaaccgcc gcgagcatgc ggtcgggcag cgtcgaggtc gccacggcga tcgagacccg
     3901 ccgtgccgtt aagcgtcgga ctgcctttct cgcacatctt ttcagtcgga attgtggctt
     3961 ggggctcggt tcgcatagcc acatcttagc ccggaaccgc ctatctgtta acaacccttt
     4021 tgctggcgca agacggctta ttccgacagc ggctccacgg atacgtcatc aacggccacc
     4081 agccaacgat cctttggcag gcgggagctt gctagatgaa ggcacaggat cgggccgtcg
     4141 cagggggctg agaggtccag gacgacttcc gtccattcct tgccgatcgg tttgcccggg
     4201 gaacgccccc cacgttcttc gtcggggtcg aacagagaag ccgtcatcat gaccgccacc
     4261 gcctgatcct gtggtccgaa gtaacgtgca aaccagcgga cacgaatcct tccacctttc
     4321 gccgcccgga cggggacttc gatgcggtcg tcaaaagctc gcttggacga actcggcaac
     4381 tgtgccagga gcagggcgtg accgccgccg tggcagtact ccttgccgag tgctccgccg
     4441 tcacggcaaa tcgacggtcc cgtgacggtc cacgcccagg gaatacctgc tcgaaacaag
     4501 ctttcaaagc ctccgttcgg aatcagtcct ttgggagcga tgaacggcgc acgtgcaatg
     4561 ggaataccct gtttctcgcc gacgattgat gagataaccg ccagcagagg cgtgtcgggt
     4621 ggcagaccgg gcagggcgac gctgaggata ttggggtcgc ctttctccga gatcacacgc
     4681 ttgccctctt cgttgaagac ctcgatcagg ctgggcgggt cttcttgttc tgaggccgaa
     4741 gcgattaggc cgttgatcac cgggacgccg tccggctgaa gcacgaaccg gagagtgaca
     4801 gggcgaggag tgcctggccg ggccagctcc tggaaggttt gaaacgcggg gcgcggccgc
     4861 aggttcaggt cgcagaggcc gaagccgagg tgcaccggct caaaatcggc gatggccagt
     4921 tgttgagcga tgagaacatc cttctggtcg gaacgactga aggctttcag gaactccgcc
     4981 gtgccgtgct gttggacttc gatgcccatt tcggcgacgt tccagccgta ttcggtgatc
     5041 cagatgggca ctcccgcgtc gccataccgg gccgcgacgg cccgaatcgc atcgagcttg
     5101 tgaatcagct cgtcggtagt ttccgtcggc cgcttggcgt atggatgctc ggcaatggca
     5161 tcccagagtt ttgcgttgtc gtatttgtcc ctgcgcagtt ggtagcactg ctcaacgaag
     5221 atcggcgcat ggccgtcggc gtcgtccagg ccgccaatgg ccacttgggc gacggggtcg
     5281 acggccttga tcgccttgaa gcaccgttgc agccacggca gatacgtgtg ggccatccgg
     5341 gcgttgtcgc acccctcgtt gatccaactg cagccgttct gctcgttcca gaattcatag
     5401 tatttggcac gattcttgta tcgctcggcc atgcgggtga tgaaggcggt gaagtcggcg
     5461 gcgtgctcct cctttggtgg atgattgtga gcgctcttgc cggtcgggct gacccatgca
     5521 gggatgttgc agatcagcac cacgggctcg atgtggtatt tggcgcaaag ctcaatttct
     5581 cggtcaaggc tcgaccagtc ccacttgccc ttttcggcct caacttcccg ccagctcgcc
     5641 agcacgcggg ccgaccggct gcccgcctca ctgatccgct gaaaaagcag ctcgtcgtag
     5701 cggacgaagt cactgccctg tcgtggtgcg ccgaccgcct ctcggttgcc gaccggattg
     5761 atgctgaaaa tgagaggccg gtcgattgtc tcggcccacg cagacgcgct cgcaagcaga
     5821 acaccggcgg ccgacaggaa caagcatgag cgggtcatca ccttaggtcc tccttaccga
     5881 aaatcctgtt ctgcacaccg cgccgattcc tgtatggtct ctcagggtac acgcggagtc
     5941 gagcagcggc aattccaaag gtcgctggcc gaggcaggcc ggatctgata cgctggggac
     6001 ggttaaccgg gttcgctttt tcttgatcag gagcatgtca ctcatgcgca gtttcgcctt
     6061 cagaatgagg atattgttgt gggtcgcggt cgcgatctgg tcgcagttga ccggctgcgg
     6121 gccccagacc gtgacgaagt ggtataaggg caatacccac acccacacgt ggtggagcga
     6181 cggcgacagc ccgcccgaaa cggtggtcaa gtggtacaaa gaccacggct accacttcct
     6241 ggtcctcagc gatcacaacg tgctcagcga gggtgagagg tgggttgcgg cggacaagcg
     6301 acgaggcgaa gccgcgagga tctacgagga gacgttcgga aacgcaaagg tggtcaaacg
     6361 ccagggcgcg gacggccaga ccgagtacct ttgcgcgccg ctcgacaagg tcaaggctca
     6421 attcgaggaa ccggggcagt tcctgctgat ccaaggtgaa gagatcagcg acagcgccga
     6481 gggcaaaccc gttcatctga acggggtcgg cattacgacg gtcatcccgc ctcagcgggg
     6541 cagcgatatc ttcgtgacgc ttcagaacaa cgtcgatgcg gttcttgccc aggaggcaca
     6601 gatgtgccac ccgatgctgg ttcacgtgaa tcatcccaat ttcggatggg cgctgcgtgc
     6661 cgaccagatg gccaggctgg aagggtcggt gatgttcgag gttgcgaatg gtcatcctgg
     6721 ggtcaacaac ctcggagacg agacgtacat gtcgaccgag cggatgtggg acgtggtgct
     6781 gaccaagcgg ctggccgagt acggcctgcc gatcatgtac ggactgggta ccgatgatgc
     6841 ccacaatttc gtcgcgaccg gcggggcggg tccaggtcgc gcctgggtcg tggtgcggtc
     6901 ctccgctttg acggccgatt gcctcatcga ggccatgtat cggggcgatt tctacgcttc
     6961 caccggtgtc atcctcaagg acatccgctt tgccgacgac cggctggaca tcgaaatcga
     7021 accccggccc ggcgtgggct acaagactca gttcatcggc acactggccg gctacgacga
     7081 caccgtggct tatctgccga gcgacccgaa gcgcggcccg acatgcaggt atagtgacga
     7141 catcggtcgg gtcctcagcg agcagaccgg cacgatcgca tcgtacaaac tcaccgggaa
     7201 ggaaatctac gtccgcgcga aggtgatttc cacggccaga catgacgttc cggtggtcgc
     7261 gggcgatttc caggtggctt ggtgccaacc ggtgcagccc ggctgccgga gcggcggcgc
     7321 tcgctgagca accgtcggcg cgctcctcga tcacttcatt gcatagacgt actggaatct
     7381 cgcattgggg gcggtgtcgc cgttgacggt gaattccatg acgtcgttgt cacactgcat
     7441 gttgtcggcc catttgaagc ccagacgcag cggcgaatcg tcccggggcc gcagaatccg
     7501 gcgcggcacc gccagttcca gctcgctgcc gctgacacga taggggactt cgtcaatggc
     7561 cgtccagacg ccgttcgtgc ccgctttctc gaggacggat ttcgtctggc cgcgaagcct
     7621 gcggtttacc cggaaatcgt agctttccca tccggtcgca agatcattgt cggcgttgat
     7681 gtagagcagc atccagttcg gatcggtact cggagtgatt accgaagcgg ttcgagcgta
     7741 gaagcagacg acgtggtcgt ccgccgctac cttcaaaagc acgaagtcgt ttcgcccggt
     7801 cttgttgaca tagcgccctg catcatcata acccgggtgg tccctgtgca tggtgtcacc
     7861 ccgatcatcg cggaacccgg gttgtacgcc cgcccaatcc gagaagtcgc cgtcgatctg
     7921 aatggtggtt ggcccggaag ctgtgggcgc ggggcgcacg cccttgaacc gcctgattcc
     7981 gctcaccatc tggtagtagt agttgtccca atgcccacct ttcatcggct cgatgtcccg
     8041 gctgtactct tgggtgaact ggtcaacaaa catgaccggc tcacgcacgc cgttgaactc
     8101 cggcagacgc atcgcaatcc attcgttcca accggtgatg aagatgaact ccgggtccag
     8161 ctccagcgcc cgcttccact gctccgcgaa gttcaggccg tgcagcacgg cgtccgggcg
     8221 ctcgtccttg atgccatcgt gccaactgcg gccgtaggtg ttgggttcgc tgaaggcgca
     8281 gagccgcttg ccgctggcgt tttgggcgac gctcaccgaa acctgttcca cctcgtttgt
     8341 gtcgttgtag tagggcgcct gcggatggat ctggagccat tgccactgat tggggccggt
     8401 ggggccggaa aagtagtcgg gcccatgcgg tcggaaggta aaagtctcct gcatctccgg
     8461 cggggcgctt tccttccttg ccagtatcag cggtttgcct ttccaacgga accacaggtc
     8521 ggcgtaatct cccttcttgt acaggtcgtc atacaagcgc gacagtactc gcctatcgtc
     8581 tccaaacggc gcaaaaaaag cgatctgcgg cgtcctgccg ccgtcccggc ggacctgcat
     8641 ccagacttcg cacagcttcc ggtagacctt ggggtatgtc tcttggttgc tcacgtcgaa
     8701 aacggcgacg tcaacaagcg cgtcggagag catctgagcg tgtttgcgga ggatccaggc
     8761 gtcatcgccg aggtagtagc cgaacagcgg ctcgccccag tggtgaaagt ggtactcagg
     8821 gccccacggg ttggtggtcg gccgcccgag ggcgtccggc tgcgaggcca ggatgcgcgt
     8881 gatatcgtac ggcccctcgg tccggtgttg cccaagccat agaaagtaga agatacccac
     8941 gctcttgccg gcacgagggc cgccgacttg gtcgtggttc ggggccgcac gtccaaggcc
     9001 gtcaacggct gcccaagtat caggttgcgt atcgatgaac tcggcggcag cgttgcggtt
     9061 gactgacaac ggaagcagga cggatgcaca tatcgccatt ccgagcgcgt ctttcatgcc
     9121 gagcacctcc ccaagcataa cgttctgatc ctcgcttttt ttctgctgtt tcgcggcccc
     9181 gtggccgaag attcacccga caggcccgcc ccacaaccag tccgataaca taggcagggc
     9241 ccggaatgcc tgcaaccaac cgcgttacga cgtgtctatt gagaggaggc aaggtctgcg
     9301 cccggcttgg ttgccagccg gtgtacgttg gcggagaatg gaccttccgc gggcgtttgc
     9361 cgtctttttc agtgatcgaa agcgagccca agagcgagga tgaggttcgg tgggttcacg
     9421 tctgatcagt atcgtggtgc cggtgtacga cgaggtcgac tcgctggatg ggcttgttgc
     9481 tgagatccgc gacgtggccg cccggaatga cctggaggtc gaagttattc tggtggacga
     9541 cggcagccgc gatgcgagct gggagaggat cgtatcgctt gctcgcaacg acaagcggat
     9601 cggcggcctg cggtttcgtc gcaattgcgg caaggcggcg gctctgatgg ccggcttcgc
     9661 cgtcgcccgc ggcaacctgg tgttcatgat ggacgccgac atgcaggatc ccccggagga
     9721 aatgccgcgt atgctcgcca agctggacga aggcttcgat ctggtcagcg gctggaagcg
     9781 catccgccac gacccgtggc acaaggtcta cccgtctcgg gtgttcaaca agatgattgg
     9841 atggttgacc ggcgtccggc tccacgatca cgtctgcggg ttcaaggtca tgcggcgcga
     9901 agtggccaag gatcttcggc tttatggcga gttccaccgc ttcattgccg tgttctcggc
     9961 ggcccgcggt tatcgagtga cggagattcc gaccctgcac cgggcaagga ctcgcggtgt
    10021 tggcaagtac ggtttgacgc gtttcttcaa gggttttctc gatctgctca ccgtgacaac
    10081 gctgacccga taccgctggc gaccgcaaca cgttatcggt gtcacaggtg tggtcgtcgt
    10141 cttgctgggc gcgatgttgc cgctccttgc cgtgatcgcc gggcagatgt cgtcggaact
    10201 cggccgcttg aatgaatggc tgggccggcc gtttgaaacg atcagaggct tgacggcgat
    10261 gcttgcctgg ctcctcctgg tcctgacgcc tgggctggtc ttgatcgcca tcggactggt
    10321 cgccgagctg gtggtagcag agcgtccctt ggatcgtctg tacgaggtgg ttgagaaagt
    10381 gggctggtgc gccgagaccg agccggccca aggctgaaca ggaacgtgtc ttaaggcgag
    10441 ggatggtatg caggatccaa caagaccggt caatccgttg ctgagagaga ccccgcccgc
    10501 gggtgccagc gttgtcgatt ccagcacccg ccccccggag aaacccctgt tcacgccgcc
    10561 cgtcccgctt cccggggtca cgaagcgccg catgccgacc gacgtgacgg ttgttttcat
    10621 gatcttggcg gcgatggtca ttagctttgc gacattgacg cgaaccaaac tggagcactg
    10681 cgttaacgat ggttcgcgtt ggaacaccgt cttttacctg gtcgagtacg gcacctatga
    10741 atatctgccg gattggggtg aaatcaaggg tgtgtcgaag aggggtgtct cggatcccga
    10801 tgaacacatc gcaagacata actccaacag cacgccggaa gacaaggccc ggatcaaggg
    10861 gttgatgcgc aagtttggcg accgctacta tcgccacgtg tgggacatcc cgcccttctc
    10921 gacggttgac atgatcagcg tgaaggacaa gaacggccag gaccacttct attccagcaa
    10981 gcctccgctg ctgtcgacct gcgtggccgg ggtggtgatg gggcttgaga agatcgtctc
    11041 accgatcatg gccatgcctt cggtccatga gtatctgacg gggtcgcggc cggaggtccg
    11101 gtccggagcc gccacgcagc cggccggcac gcagccgtcc cagacgccct cgcccggctc
    11161 gttcacattt cgcgagcacc ccatgttcta catgcgtgcc gcggtgcttc tggttcaact
    11221 ggtcccgttc ctgatcatga tctgggtaat tcgtcagcgg gtgatcgagc agacggattc
    11281 tcctttcgtg cagaacttct gcgtgggtgc ggcggccttg gcgaccttcc tgacttcata
    11341 tgccgcacct ctaaacaacc atattgttgc cgcttactgt gccatgtttg ccctgcatgc
    11401 gatgctgcgc gtctggtatg atggccgtcg cgagtggtat tggttcctca tcgccgggct
    11461 gttcggcggg ctgtgccacg gcctggaact gccggcaact ctgctcacgc tggctattct
    11521 tgtcgctctg ttctggaaag agccgcggcg ggcgctggtc gtcggcctgc cggccatgct
    11581 cattcccatc gccgcgtcac tctacaccac gtacctggtc accggttcgg taacgcccat
    11641 cccgatgagg ttcgacgagc cgaacggtcc ctttttctat gatggcagtt actggcaccc
    11701 caacaactat tacacccccc gaggcgtgag catcgatttt ctccaggagc cgaagcagaa
    11761 atacatgttc cacctcctgg tggggcatca cggctttttc agtctgacgc cgctgttcgt
    11821 tctgagcctg atcggcatcg gacggcacat ggccggcata cgagcaaggc gggcggtcct
    11881 tgcctcagtg gtgttggcgg cgatcttcct tgctgcggca attctgtgga aagccgatgc
    11941 cggaggttcc tcgctatcgt ggcccaggcc cgacgcggcc cgggttgcgt caacgcacgc
    12001 catggcgggg gacgtgtcgg ccgcttctgc cgattctgcc gacggctctc ggttcactcg
    12061 ctggtgggcg gacttcaagc tcggcctggc atgcatcaag acggccgtga gccatcccgt
    12121 cgcggcgatt gatcaggcgg ccgacctgct gtggggccga ggcttcctcg cgttcatcgc
    12181 gatcctgctg gtcgtgaacc tgggaatgta ccttggagaa cccgaccaga aacactcgct
    12241 tttggccgcc ggaacgttgt tcctgttcct tgcggtgctc ttgttttata cattccgcac
    12301 gcacaattat gcgggtgttt gccaggggcc gcgctggatg ttctggatga tcccgttgtg
    12361 gctattgatg ttgcctgccg gcatcgggcc gctggcggag cggcgagtcg ggcggtggtt
    12421 gtgctatctg tgcctgttcg tgtcgttgtt gtccgtgttc tggggattgc ccaggcccga
    12481 tgaagacgtc agagccaccg accgcccgtt tacgtcctcg tggctccacg attacatgcg
    12541 atggcgaaac tatattgact actgacgtga atcaggaata cgaagcactg cgacgggagt
    12601 cctttggctg gatcaaggcg gcggcacgaa ttgccctgga acggctcggg agggctgacg
    12661 tgactcgcaa gcacgacaag tctcctgtga ccgacgccga tcatgcggtc caggatgctc
    12721 tgttggcgtc catcgccaag ttgtatcctc aggacgccgt gataaccgag gagattcaat
    12781 ctcggccgga tgtccaccag cccgtttcac gctctggacg ttgctgggtc atcgacccca
    12841 tcgatggcac ccggaattac gttcgagcga ttccagtatt cacggtctcg gtggcggtca
    12901 tggaaaacgg acagccggtc gtgggcatgg tctacgatcc ggtcgccgat cggatgtatt
    12961 cggcctcgat cggggccggt gcctgggtca acgccgaccg aatcgaacgg cgcgaagtgc
    13021 cgacctggaa cgaggtctac atgggtatcc ccacgagcaa gcacgagcag ttgcccccgg
    13081 tggtgcaccg ctggatcgac accatggtgg tccgcaactt tggctcgacg gccatgcatc
    13141 tatcgcttct ggcgaccggt tcgctcgatg ccgtttactg caagaagtca aagctgtggg
    13201 atatcgcggc gggagccgta attgcctgtg agacgggcgc tgaagttttg tctctggacg
    13261 gtcgtgagca ctttccgatg gatctcagtc actacgccaa cgagccgctt cccatgatcg
    13321 ccgccaggcc ggacctggtc gagagactcc tcgctgagta tcgcaccgcc cagatgagct
    13381 gaacctcttt tgtgccggga caacagcggc aagccgccgg ggcgtaacag actcaaatga
    13441 tgtgcacaac cgtcaagccg ccgcctgacc gtgcggcagg tctcggtcgc cgcagcaacc
    13501 tcgacgttgc cccaccgcgc tcgcggcgtc ccgcatgtat gttcccacag gggtgcagaa
    13561 gaaagggctg cggaaggctg cacccccggt cttgggccgc ttacttcttt tcctcgaact
    13621 ccgcgtcgat cacgtcatcc gccttggcct gcgatgcccc acccgcagtt gcaccggcag
    13681 tcgctgcctg gcctgccgtt ccgcctgcct tgcgagcaac ttcctcgtat atgatcttgc
    13741 caatggtctg cgaggatgtg gccacttgct ccatggcccg ctgaatggcg gccttgtcgt
    13801 cgcccttcat ggcctctttg agctggttga tcgccgcctc gacgtttccc cgctcgttcg
    13861 caggtacctt gtcgccatgt tccttcagtg tccgctcggt ctggtaaatc atgttgtcgg
    13921 cctggttgcg gacctcgacc agctcgcggc ggctctggtc ttcagccgca tgttcctcgg
    13981 cctcccgccg catccgttca atctccgcct tgctcagacc acttgacccc tcgatccgga
    14041 tcgactgggc cttgccggtg ccaagatcct tggccgacac gttcagaatt ccattcgcgt
    14101 cgatgtcgaa cgtgacctca atttgcggga tcccccgcgg tgccgggggg atgccggtga
    14161 gattgaagcg tccgagcgac cggttatcgg cggccatttg ccgctcacct tgcaggacgt
    14221 gaatggtgac ctccgtctgg ccgtccgcgg cggtgctgaa gatctccttc ttgctcgtcg
    14281 ggatggtcgt gttcttctcg atcagtttgg tcatcacgcc gcccagagtc tccactccca
    14341 gtgacaacgg ggtcacgtcc agcagcacga tatcatcctt ctcgccggcc agaaccgccc
    14401 cctggatcgc ggcacccacg gcaaccacct cgtcggggtt gatgcttttg ttgggctctt
    14461 ttccgaagag ttccttcacc aaccgctgaa ccgccggcat ccgggtcgac ccgccgacga
    14521 ggaccacttc gtcgatatcc ttcggtgtga gtttcgcgtc ggacagcgcg gtcatcaccg
    14581 gctgacggca gcgatccgtc aggtgctggg tcaactgctc aaacttcgcc cgcgtcagct
    14641 tctcctggag gtgcttcggg ccggcctggt cggcggtgat gaacggcagg ttgatcgtcg
    14701 tctcctgcac cgtggacaac tcgcacttgg ccttctcgca ggcttctttt agtcgctgca
    14761 gggccatcgg atccttgcgc aagtcaatgc cttccttccg ccggaattcc tcggcaacgt
    14821 aatcgatcag caccttgtcg tagtcgtcac cgcccaggtg cgtatcgccg ttggtgctga
    14881 gcacctcgaa cacgctgtcc ccgatatcga ggatcgaaat gtcgaacgtg ccgccgccga
    14941 ggtcgaatac ggcgatcttc tcgttcttct ttttctcgag tccgtacgcc aaggccgccg
    15001 cggtgggctc gttgatgatc ctctcgacct tgaggccggc gatctccccg gcatccttgg
    15061 tggcttgtcg ctgcgcgtca ttgaagtaag ccggaacggt gataaccgcg cgcgtgacgg
    15121 tctcgcccag atagtcctcg gccgtcttct tgagatcacg cagaataatt gcggagatct
    15181 ccggcggagt gtactgcttg ccccgtacct ccaccttgac cagatcctgc ggcccgccga
    15241 cgatcttgta aggcaccgtc ttttcctcgg attggacttc gtaatgacga cgccccatga
    15301 accgcttgat cgagaaaatg gtattctccg gattggtcac ctgctgatgt ttggcgatct
    15361 ggccgacaag ctgctcgccc ttgtccgtga agcccaccac tgacggagtc agccgggagc
    15421 cttgggcgtt ggtcagcacc ttgggttggt cgccctccat gatcgctacc accgagttcg
    15481 tggtgcccaa gtcgattccg atgatttttg acatacgttc catctccttc gaacctgcgc
    15541 ggaaagcctt gttgccagcg tcccgcgcga catgttctcg gcactgcgct gtcgagagtc
    15601 cttggccacg cgcagtctca ccgccgcgac ccgacttccg ttcagccgat ctctagttac
    15661 agcaagaaag atgccaaccc tccagctctt gccttctata ccgtaagctc tgtttgtgca
    15721 aatgcttaca ccatattctg cggccttgag atcttggctt tgggactgcc agagtggcag
    15781 gcagatctgc tcggggttgg cgtcgccaac gtttcggggg ctaaaatgta gacatgtcac
    15841 tgttgcacaa gcccttggag acggtggggg ggcacggagc gcctgcggtt cggcagattt
    15901 ccgtcttcgt ggagaaccgg ttgggccaac tcctgagcat tacgcaagtc cttgagaaaa
    15961 aagatattaa gatgctggcc ctgtcgattg ttgacttggt tgactgcgcc atcgtgcggt
    16021 tgctttttga tgccgcagat gaggcgattc gcgttctccg cgaggcgggt ttcactgtga
    16081 cggtcgcaga ggtggttgtc gttcgcctgc cgaaaggcaa acgaggcctt ttgaccgtct
    16141 ggtcggcctt gcttggagct gagatcaaca ttgcgtatgc ctaccccttg ctggcctccc
    16201 aaaaaggatc cgcgatcgcc atctacgtgg acaatgtgga cttggccatc gataccctcc
    16261 gtacccacaa ctttgaggtg ctcgatgaat cggatttgca ggaagagacg tagactgcca
    16321 aattgaccgg cacccctgcc atcggccatg atctaagtgc ctgtcaagcg gtctgttacg
    16381 tacaatcggc gcggtggcac tccgattgca gcgacctcag agccgcgcgc ggtcgtacaa
    16441 tttttcgacg acctgctgca aggacgttcg ggggcaacga aaggaggttg acatgcttgg
    16501 ttggtgggat caccagtact tcttgctcgt tggcccaggc attctgctgg ccctctacgc
    16561 gagcctcaag gtgaagtcaa cctttgcgag agccaaggaa atgaccactc gcaagggctg
    16621 gaccggccgt gatatagccc aagccatcct cgacagcgaa ggaatccacg atgtggccat
    16681 cgagccggtg aacagttacc ttggtgacca ctacgacccg cgagaacggg tgctgcgatt
    16741 gagtcctgat gtctaccacg gtcgatccat cgctgcggca ggtgtggccg cgcacgaggc
    16801 cgggcatgcg attcagcacg cccacggcta cgcggcgttg tccctgcgga acagcatcat
    16861 tcccctggcc agcattggct caaacttcag tttcatactc atcatgattg gcttggccat
    16921 ggccctcaag tcgctggcgg tcatcggttg cgccctgttc acgatggtcg tgctattcca
    16981 gttgatcaac cttcccgtcg aattcaatgc cagcacgcgt gcgcgtcagg ctcttctggc
    17041 caatggtctg gtcactcaag tagaggacga ggaagtaggc cgggtccttc gggccgccgc
    17101 catgacctat gtcgccgcca cgctgacggc tatcctgacg ctgttgtact acctgatgcg
    17161 ggccggcctg ctgggtggtc accgcgatga ttaatcgcgg acgctgaccg agagagcctt
    17221 caacttccga agatgaaagc caacccggtc atgtactcgc cccggacctt cttgcagtag
    17281 cgaacacagg ccttggttgc gaaggatgcg tggggcaact gtacgacgat gtcaaccacc
    17341 gaatcgggct cgaagtaatg tgcggagctc atccccaggc ccccttcgga aatgttcatt
    17401 acgttacccg tcaggctatc gctttgccgg ctgaacaccg gtcgcaattc aactgtgccc
    17461 cgtgagctgt atcgtgggtg tcgtctcgga tcgttacggt cgtcttgttg atgaggagcc
    17521 tcatggggca gttgccgcac gatctcggcg ataacgtctc gggtgagctt gggaacctct
    17581 ccgatgacca tctctcttgc tcgcactgtg attcgccctg gaggccgtct ttgtcctggc
    17641 accatttgaa ggcctggagc acgttgttct tcggcgcctt agtcgtcgaa gacgaactga
    17701 atacccatca gatactccgc ctcgccgtcc atctccttgc agtatcgcac cttcgcctgg
    17761 ccatagaagg tctgctcggg cagatgcacc gagatatcca acagcgcgtc aggcgggaag
    17821 tacgtatccg tcttcacgcc cataccgccc tcactgatgt ctcgacaaac ggcaaaccac
    17881 tgctggttgt tgtcgcccgt cgcatacctc agttcgacag tgcctggaaa tggccagcgc
    17941 ggcgcccgcc gcagctcggc tccatcgtat ctctgcccgg ccgtctccat ccgcttgcgg
    18001 atgaggcggg cgatctgctt gcgtgtcagt ttgacgatct ctcccgtcgg catgacactt
    18061 ccccctgatc gtaactcggg tgcggacggc gtcggagctc acccgctgtt gctgtccctt
    18121 atcggaccga tggcgagagg ctcggcctgc tagtttggtc ggcgtttgag cggcgcagct
    18181 taatgcttaa tccgccgttg ttctggagcg tcaggctcca aacctgcccg cgctcgaccg
    18241 gcacatagac ggcctcccct tgagcgaccg taatgccttt tcgggcggcg aggtccggat
    18301 cggacgccaa gtcccagaac tggcgccaca attcctgctc aaagggcgtc ggtgatgggt
    18361 tcacacgaaa gatattgggc acatcgccgc tggggtcgac gggaaacccg tcgataggat
    18421 tctggtgttc tccgtagatc cggcgaaaca gcgccagact cttgccgcga agggcgtctc
    18481 cgagagacac gtactcaggg tcgaacttca gcactagggc atcgaagaaa atgacgtcgt
    18541 cattgatcat gaatcgccgc gacggcaggg cgtgtccttc gcggtcgatc tccacgaatt
    18601 cgatgatggt gatgtccgtg tcagaaggcc ggccgttgaa cacctcgcct tctctgacct
    18661 gatcgaccac atagacttcg gccacgcggt cctctccggt cagccggctg actgcctgtt
    18721 ccagagcgat gcgccgtttc tccagttcct cgttttcgcg gcgaaggcgc gcggtttcac
    18781 ccggcgacag catccagagt gtccctgcga tggccgcgcc gaggacggcc aacccgaatg
    18841 tccattgcag atagcgcaaa gcaagcccct cgcaaggacg atccttgcag atagcccatt
    18901 gcccgagtga taggcccttc tgctcaaagg cgccacgctc tcgcggcaag cgattattta
    18961 tacgatatgg atcggctcaa gggtgcgaaa acggtaacct gcgcgtccga gaaggcacga
    19021 gaagcaaccc aacagtcagg aaaaccacct cacccatccc gagcgccccg cagcctgtgc
    19081 cggaaccgga cgaacttccg ccgtctgagc cgccatcgct ggggccccct ccgtcaggca
    19141 aaggcaggac tgcaagagtg accgtgccgg gtatggagtc ctctcggccg tcattcgcgc
    19201 accactggaa tgtctcggtt ccgacagcgc ctgcatcggg cgtaaaggag aaggtgaagg
    19261 gcggcccgat gctggcagcc gtataggtcg cggtcaggct ggcggtaaca tcattgatct
    19321 tgagcacgcc ggtcttgggc agggccgtca gacgcaacgt gaccttgtca ccgtcttcgt
    19381 cgctggggct caggacgccg gtgatgatgg tgccgcagaa gccggagacg gcagagtcgg
    19441 gtgatagagg cggagtgttc accgcgacgg cgacgaccgc gcggtccgag ccggccacgc
    19501 catcactgac ccgaaagtga aagctatctg ttcctcgata gcccggagtc acgctgtata
    19561 agacctcatt tacgcctcgg gtaagcgtct tgggcagatc accttcctcg atcagaacct
    19621 cgcccgtcga cagggtgccg tgcccgggca ggctggcgat ggtgtattcg atgggatccc
    19681 ggtccgcatc gttggcaatc aggcgtatcg ttacagatga gtcgggcagg agcgtgacgc
    19741 gttggtcttc gggcacggga gccgtattga tctggacggc cacgctgatc gacccggttg
    19801 tgacgtagcc atcacttgca gtgaattcga aggtgtcgag cccgtgataa tcggtatccg
    19861 gcgagtaggt gagcttgttc gacgacgttg cgtaaggaat gtcagcatcc gtcagaatgg
    19921 ccgaacccga cttgagccgt gcgtggcccg gcagcgagac gatgacgaac gagagggtat
    19981 cgccatccgc gtcgctcacc ggaagggtga tgttcaggtc cgtgttcggt ggcgtagtca
    20041 gttcggtcat tccggtaagg ggctcgcggt tcacggtcac ggtgatcgtg gcagcggtgg
    20101 agttttgcgt gccgtcgttg gctttgaagg tgaaggtgtc cgtgccgtgt gccgtggttg
    20161 tggccgtgta ggtgacggtc ctgccggcag cgggaatcgc gtagggaagg tctccggccg
    20221 caagcgtggt cgatccgacg gccagcgagc ctttggtggg cagcgtgctg atgatgtaag
    20281 tcaggggatc cgagtccgca tcggtcgccg agagggtgat ggttatcgtt tcgcctggct
    20341 ccgctttggc tgagccggcc gcggcaacgg gtgcggccgc catcagggtc gagggaacgc
    20401 cggccgcggc caggccgagc cccaggacca aggccaatcg cccgagactc ctcaaagcaa
    20461 tcggcaagtt gactgtattc atggtgaccc tgcgcgcaac aatcccttca gacggaaccc
    20521 tgcgcaggat atcggcccgt caataaggtg acttaaggga gccggcgtcc gcggcgatta
    20581 tcgggcggac cggctcgcgt cgggcagcgg cttgctgcgt ggacgtcagg agcaacctgg
    20641 cattgccttg cctgcgaatt gacggcaccc ggcccgaaga aacaggaccg gcatcttttc
    20701 ttggtcccaa ctcagggtga ttcatcacag gtctcgtcgg gcgggatgcc cggtccggag
    20761 gcacatcgct caaatacgag gtggtcggct tgatcaatgt agttgtcttc atcgacatcc
    20821 aggcacttgc atggatcgtc atacgcccaa ttgccagggc ttgttccggt gtaacagcgc
    20881 tgaaatgctg agaaatccac ctggtcgacg tcaaagtcac cgtccacatc aaagcgcggg
    20941 tcggaacagc gcgacaccag atgcaatcga gtctggaaag cccagtcctt ggcggccgcg
    21001 tcatcgtgaa aaccctgatt gtcagcgccg ctgggctcgc ccaaatcccc cttgacgcct
    21061 gtggaccagc atctcagggg agcaggagct tcggtcacct cgagatccca caggtacgag
    21121 ccgtcaaccg ccaggggggc aagagcagga tagctgatac agcttcgctc gtcctccgta
    21181 atcgtgtcaa gatccaacat cacccaaccc ggtccgccgg ccgtcctctc cacgcgccac
    21241 gttttgatcg ggccatcgat cagcaccgtc cgcatccagc ttatgttcca ctggtagaga
    21301 gatacgtaca tgacgaaaga gccgttactc tccatgtaca tgccgaacga atcaaataac
    21361 ccgctcagtg gcggtcgctg cgcttccggc aagctcgacg ccggagtaaa ctgctggctg
    21421 atctcacttc cggcgatcag gtccttcgac cagactctgc cagcgtaaag gtcgaactcc
    21481 ggcgggacgg ccggatcggg ctgtgcaagc accgcagccg gaagtagcaa tacgatcagc
    21541 gcgaacgtat gatcaagcat cgatcgactg gtcgctctca tatcattgcc tccaggaccc
    21601 aaggagatct tccgagcatg ttcttcgata cctctcaagg gcaggctacg gtgttacact
    21661 gcgtttgcga tccctgcggc gttccgcccc gatcggagca tacaaccgaa gcgagattct
    21721 ggcagctcga gtcagcgaag cagcaggctt gtgggtcatc acaggccgga tcagccggaa
    21781 cacccgatcc cagagcgcag gcgtcgaagg ccaagtactc aacactgtcg atatcatcgt
    21841 cgccgtcgat atccagacac ctgcagggat cgtcataggg ccaagtgccg ggaatcccag
    21901 cgcccgtata acatgattgc aggaccgtga agtcgtcccg atcgacgtcg gtatcgttgt
    21961 ctgtgtcgta acgaggattg aaacaggtcg gagtcagggt gtgtagtcga gtttgatagc
    22021 tccacgttct caccgctcct ccgccatgga tgccgatgtt cccaacgccg caatcgacca
    22081 ctggagtccg ggtggtggcc cagcattgca gcgggctgcc gctggcgcgt gtcaccaaca
    22141 gccgccagaa gtacgacccg tcaagcggca gagggtcgag ggcgggatag acctttgggc
    22201 cggtgccggc atcgttgcag taccgcaggt ctacgctctc gcttcccaga tcgaacatga
    22261 tccaggcttc gcccgtttgg cccactttga cgacgcgcca caccttcatg gggccgttgg
    22321 cgatcgtcgt ggaccagttt gtgtcccacg tatagaactc catatagaac tcgtaatccc
    22381 ccgtgctgtt gatgtaaacg ccgaaggagt cgaacccccc gatgaccgga gggcgctgag
    22441 cttccggaag actggatgca gggctgaatt gctggcccgc ctcctgaccg gctccccagg
    22501 ccttttccca cacggtcgcc gcataaaggt catagtcggg tggatccggc gggccgggag
    22561 caccggctgc gaggcccgac aacgagaaga ccaccattag cgttgtcgcc aacccgaggt
    22621 ttgattcacg cattgctatc atgtttcctg ttctccgaca ccacaaaggt tgtccggccc
    22681 atcgaagcca tcagcttgca tgggaatcat gcgcaggctc ataaggcttc agatgcgtgg
    22741 cctcacggct caacgcgtcc cagtcgcgtc ggcaaagttg cccgctaccg gcgtctgcgc
    22801 attgccatgc aaccggaaaa acccagcaga gccagagccg ccggctccgg cacgtagacc
    22861 gccgtgacaa aggcacggtt ctcctgaccg gggaaggcgc cggcatcaaa ttgagtgtta
    22921 cccacgatgg tggtgtctct atttccaggg cgatgccact tggcaaactg gatgtttccc
    22981 ccaccagtcc acaccagcct ccagacgtat cccgaatcca ttggagatgc cgctggccac
    23041 ggctggggat tctcagggaa ctcgaggacg agccaacccg cggccggctg gccgttgccg
    23101 aagctttgcg acgcgatcaa gttcgcgggt tgcacggtat tgttccatgt tgtgatggcg
    23161 tcaacgccct caacggtcct gtaaagatag aggttcaagt cggtgattgt gccgcttccg
    23221 ggcatgacgt aaacggcgat cttcgtgaat gtgcatccat tcgggggttt atcgagcctg
    23281 ttgtcgccgt tgaccggatc cgttcgcgaa ctgaaccaat ctgccgcccc ggtgttgcgt
    23341 gcctgggtac cggtctgatc gtagagcacg taccacggcg gaggtgcgtc aagccccccg
    23401 ggatccgtga tgcggattgc caggaccggc gatgaaaaga ccgtcatgcc gattgtcgcc
    23461 agacaggcga gtactgtgat gtgctttcct ctcattgctc ttcctcctgc tcggtgagcg
    23521 ataacaataa cccggcccac gaatgtgcct ggtaacatgc aaaaccaagg ctggcaaaag
    23581 aaggaaaggt cgagttacat cctgatccca ctgggatccg ttcggctaat ccctctccgc
    23641 cggccccctc ccgaacggcc aagagacatg cgcggaactg atgatgcaca tgcatattgc
    23701 attgtcccat ccagtcggga ggttgtcaag gaaaaaccca gacaatgtcg caggagcagt
    23761 acctgtatac cccccatctt caagacagca gacttcctat ggcgataaga ttgcctgacg
    23821 ccttcgcttt cagtttggca ccacctttcg acgatttgct ggagcgaggc ggcaacgcgg
    23881 acagacgcgt ccggacgttg agacgtcgtt tcccctgcgg ggcgatgggt ttccagtaaa
    23941 gagacgtagc ggcacggccg tgcgtaatca cagaacggcg cgtgacggaa acgttttcag
    24001 aaacagaata aattccacat cagcggtacc gcccaatcgc gcgcggtcat tcagcggggg
    24061 ccgtcgtcta cttcagccac ttcgcggcag ctttcttcag cttttccgcc atgtcggttt
    24121 tctcccaggt gaagcctggg ccggtgcggc cgaagtggcc gttgcgggcc gtttcgcggt
    24181 atatggggcg ccgcagtttc aggtgttgga tgatctccgc aggtttcatg ggaaacatgt
    24241 cgcggaccag tctttcgagc gtcgtctcgg gcacccggtt ggtgccgttg gtggagacga
    24301 gcagactgat aggctcggcg acgccgatgg cgtaggcaaa ctgcacttcg cattcgtcgg
    24361 ccaggccggc ggcgacgatg ttcttggcga tatagcgggc catgtaggcg gcgctgcggt
    24421 cgaccttgct tggatccttg ccgctgaagg ccccgccgcc gtgacggcca cggccgccgt
    24481 aggtgtccac gatgatcttg cggccggtca ggcccgaatc accgtggggt ccgccgacca
    24541 cgaatcggcc ggtcggattg atatggtagc ggattcgatt ggaccagagg tcgggccgtt
    24601 cccgcatgat gaccggctta atgatgttct cgatgatctt ggcttgagcc tgccttgaca
    24661 ggttgcctga cttatcgacg acctcttcgg tgtgctgggt ggagataacc acggtgtcga
    24721 tgcgagtcgg taccccgccg tcgtaagcga tggtcacttg gctcttgccg tctggccgca
    24781 gccacttgat ctcgccgttc tcgcgggctt cagtcagccg ctcgacgagt cgatgagcca
    24841 ggtgaatcgg caacggcatg agggctttgg tttcgcggca ggcaaagccg aacatgaggc
    24901 cctggtcgcc ggcaccctgt tccttgtgac ggcccttgcc tttggttacc ccttggctga
    24961 tgtcgggtga ctgagagtga atagcccgca ggaccgcaca actgcgatag tcaaacccga
    25021 tggcggagtc ggtatagccg atctcgcgga cggtctcgcg ggcggtggtc tcggcttccg
    25081 ccagggctcg ctccgcggcc gtgttatgga cggtgatctc accggccatc acgatcagcc
    25141 ctgtggtgac catcgtctcg agggccacgc gggccatcgg atcatggcgc agcaggctgt
    25201 cgaggacggc atcggaaatc tggtcgcaga tcttgtcggg atgcccctgg gaaacagact
    25261 ccgaagtgaa caggtaacgt cgcttatcag tgggggtaac caatccaata tcctttcgtc
    25321 acagttgtcc ggcctcggca cgaggtctta cgttgactgt tccggcccct tttccaaacg
    25381 caggagtcgg ggcactctgt caggttcgct cgcgacccga cctccagttt gggctgatat
    25441 attaggtctt tctgacgttc ctgacaagga aggccggtcc taccggcttt tctgggtgag
    25501 tccttcgaga aagcggtcca ggtaacccct cttgaggaac agattcatgg cctccacggt
    25561 ctgttcgtgg ttcatacccc acaggcagca gcgcaggtcg gtcagctcca gatccatctg
    25621 aatgatcagg acgttatcga tggtcacaac cgagggcagt tcctgaaaga gcaaagcgga
    25681 caggtggttg acgcccggcc cgccaaccga gatcaccggc tgtttgctca gtctcttctc
    25741 gttgatgtac aaaacgtcgc tgataacaag cggcttccag ggcgactcgg ggccgagccg
    25801 ttcggcaatt tcccgggcaa gctgataggc cagcgggcgg tcggcggcct cgccgcgcag
    25861 tgacgctccc gtgacgatca ggatcagatg gttcagatcg gtttgcggca tggcttgcag
    25921 tatacctcac gggccgggag gaacatacat gacctgtgcc ggcaggatcc gggtcaatcc
    25981 acgtgtgcct gctgcggccg gcctccgagc cgaccgtcga ccggtcgcct ggacaacgag
    26041 gtcgagaagc ccgcaatcag atcttggcat tggctctcag ccgttgcagg gcctgaacat
    26101 gcatgtcggg aatcaatccg tgcttgtccg gcggcacgtc cagaagcagg ttgacaccgc
    26161 ggtcgaggca gctctgaagt atatccagca aggcgtcgtc tgatctcggc ttgtcgtccg
    26221 gcgtgaagaa ccagtccttg ccgatcgggt cgcagacctc gccgggcata taacagcgcc
    26281 ggccctcgat ggtccgccat ttctcgtggc ctttgcccgg cggcaatcgc cgttcgatgg
    26341 cgatgatgtc ggaaggccag gcgtaatcca caggatagct ttcaccgctg ctgataccgc
    26401 tgttcatcat gatgaccgtc tgcggttgca gccgggcgat ggtgttgtac aggtattcgc
    26461 ggtagcctcg gccgagaacg ccgggaatgt cgatccaggt ttcggctata ggcccgtact
    26521 gggtcagcag ctcggtgatc tgcatggtct ggaagccctg gtagaccgag gtggtgtacg
    26581 ctggcagcgg tgcggagggt tgcgtcgaag ttcttcgctc cagttcggcg aatctgctca
    26641 tgccgaactc ccagagggta ttggggtccg agggggtgcg gctgccgaag cggttgtgat
    26701 tgtcccacga gcagtaatag aagccgggca gcacgcctcg tttctcgcat gctttgacga
    26761 actgctcgac gacgtcggtc ttgttgccgc tgttggccac cgtatagctg gtatgtttgc
    26821 tcggccagag gcagtggccg gcgacgtgtt tggtcgtcag caccgcgtat ttcattcccg
    26881 cgtcgcgggc gacggagacc cactggtcga cgttcagcct gtcgggagca tagacggacg
    26941 caggggcctt gccatccggc agctcgtttt ccacgtacgt gctcatcccg aagtggatga
    27001 acatgccata gcgaagagac tcccactggc gcagttgttc gagcgacaga cgctgatcgc
    27061 cctgggcggg gtccctgccg ccggtctgag cctgcgccgc caggcgcgcg gagcctgccg
    27121 cgaccgcgca gacggccgcg ccggactgcg ctgccgtctt gagaatgccg cgtctgctga
    27181 gcgtctcctt gttcatggtg gtctccatct cttggttccg ccttgtcacg gcttcgagtc
    27241 ccgccggcct cagaaccctt tgtccgctgg ccccagaatc ccttctgaag ccttcttcgc
    27301 ggaggattcc catcctcatt cgggcccgga acggattccg ggccgagcgg atttcaggcc
    27361 gagcggagca tctgacattt cagatctgac atttcagatt tgaaatccgc agtttgcccg
    27421 cctcaaaacc cttgttgggg ccttccggct cacttcggcg acacccactc gatcatcgag
    27481 atcaccgcat ttcccttgcc ggctcggttg gccaccctga ccagcagcct gccgtcggcg
    27541 gtcatgtcgc gagtgaccgg cgcgtcaatc cagcggccgt cctggaactt ctcgaacgtg
    27601 ccgaggctct tatcgccaat gaacacctcc tgctttcggc cgccctcaaa gctgtccgga
    27661 tcgatcaggt aaagtcgaag cgttccttcg gcatcctggg gaacggtcaa ctcgaattga
    27721 atctcgtggt cgtcggatcg gcagtggaag acgggcggcg gccagccggt ccacgtggca
    27781 ccttgtttga cggcggcacc gaagcccggt tctgcgaaca ggatttcctt ttccgggtat
    27841 ccgcaagcca tgtatagctt gacaccgcga gctcgcaggt cctccgccga cggcagcggc
    27901 gggggttcct ccggccagaa cattttctcg ggcgcgggga cccgctcggc ggcggggatc
    27961 atcggcggga atgggagatg cggctcagtc tggtaccagt agcaggtgct ggagaactga
    28021 agctgcgtac cgggcttgct gaactcacgg cggaacatgg ggtcttcgtt gactccatag
    28081 ccgatgtcga ccgccaggga ctttctgaag gcgatggaat cgttcagaaa gaaacggtaa
    28141 gctgcggccc ccttcataaa cggccagtaa ccgagcgtcg ggtagatgtt ttcgacttcg
    28201 gggaagcccc aactgaagcc gaatgagtct tcaattccct ggaactccac cgaaggggtc
    28261 tcttcgccgt cgacgtagaa tttctcgttc atgtccaccg gataaccgga cgttccggga
    28321 cggcggacgg tcacgttcca tccgatgaat tcgcctcggc cttgggcacg gagcgccggg
    28381 tagtcttcct tcccggtcaa gacggccttc tggttccacc atgcgtgaaa gtagggcgcc
    28441 tgcggcggca cgggcgtcga tcgccacatg acgtagctgt agcagggcat catcgcccac
    28501 agcttctcgc cgggcggctc cgggccttcg tagacgagca tgaccttggc cgacttgcgg
    28561 aagggcatct ggaaatacgc gttccagccg cgtttcttat tggtcaacgc tgtgttgacg
    28621 acttcgcgga gcccggccgg atcgcagaag aagtccacca tcgggcagtc gacacttggc
    28681 tccggctcgt cgtcccacca aattctcagc aggagatcgc ggttcaggtg atactccttc
    28741 ggtgcggcaa tcgcccggtg aggataggcg aaatggatca tggtaatcat accgggcccc
    28801 tgcatctcgg cgacaaccac ctctcgattg ttgtggaacc gtcgttccgg cggcgtttcg
    28861 atccagagtg cgttctccgc ccgcacgcgg gtctcgtggc ggacggcgga cgcgctgtct
    28921 gtccctgacg gctgttggca tccaacaaga agaaatgcga ccatgccggc aatcgaccat
    28981 gccaggtgtc gggtccgcct catgatgctg cctccatatt aggggtcccg tcttcaagcc
    29041 accaagttcc tgcggcttct gactacgcct acaaggaccg gctgcggact ttgaacggcc
    29101 ggattctagc cgtgcgatgc cccggttgtc cagacagcag attactgcca gacatcatct
    29161 ccggctggaa cctcgggttc agaatggtta tcctgtacgt tctggttttg gcaattcgga
    29221 aggagttgac tcatggcaga tcaaccgacg atgacccctc agatgcagac ctcgtacaac
    29281 acgtcgccca aagtgggaat ggccgtcgcc gcgctggtgt gcggcatttt gggcttttgc
    29341 ttcccgcccc tggggctggt ggggctgatt cttggcatta tcgcggtgat cagggcgagc
    29401 agcgatcccg ttcgtcacgg tggcaagggg atggccatcg gcggcatctg caccggtggc
    29461 gtctcgctgg tcttcatggt cttcataggc ccgatgatgg cggcgatcct gttgccgtct
    29521 tttgcccggg ctcgtgagtt ggccaaacgc accgcttgcc aggcgaacat gcgacctctg
    29581 agcatggcct tcctgaacta caccgtcgaa aacaacggcg cttttccgga cgaccccgcc
    29641 aaggcgctgg cacgtgccgg catatcagcc aagaacctgg agtgtccgag cagcggagga
    29701 cagtccaact accgctatgt tcccgggtgg tccacaaaaa acggggcgaa caagccgctg
    29761 gtttacgaac cgatcgcgaa ccatggcgac gaggggggca acgtgctgtt cctcgatggg
    29821 catgccgagt gggtggagaa gactcgctgg gccaaggtgg tggggcctta cgaggataag
    29881 tccgttccaa tctctccgcc gtgacgacga acggcgtggt cctgacttcc tggcgtggag
    29941 atgcgcgtga tgaccgtcgc tggagccaaa cgttccgatc acctgttgaa agtgttgtcg
    30001 ggctaccagc acgttcttgt gctgacccac gacaaccccg accctgacgc gatcgccagt
    30061 ggttgggcag ttgctgcatt ggttcgtgcg aaggcggcgc tgccggtccg attcgtcgcc
    30121 gggggtgcga tcttcagagc cgagaaccgg cgaatggtgg agttgcttga gcctccgctt
    30181 gagtttgtgg agcagatcga agtgcccgag ggcacggcca cggtcctggt cgacgccgaa
    30241 ccgcacgcgg tcaatcatct tttgggcgag gccggtctcc cgacggcggt gatcgatcac
    30301 catcaacacg agggccgacg gccgcgactg ccattctgcg acatcaggcc caaggtcgtt
    30361 gcctcggcca ccatcgcggc gcactacttg cgggaacaac atattgatcc cggccctgcg
    30421 ttggcgaccg ctctgaccta tgccatccgg accgacgcca cgggcttgcg gggtttttcg
    30481 gccgccgatc accgggccat gcgttggttg agtttgcgcg ccgaccatca gaaactcacc
    30541 gacatcgaga atgctccgct gcagcccgat tactacgccg atcttctgct ggccatgcag
    30601 aatacctttc tctacgagga cgtggcactg tgttttctgc cgcgggccgg cggctcggag
    30661 atcattgccg aagtggccga cctgctgatt cgacaccgcg acctgacccg ggtgatggtg
    30721 ggcggcgtgg tcaacggcga tctgctggtg tcggttcggt ccaaagtcgg tgcgggcgat
    30781 gccagcgatt tggtcgcaag gtctctcgag gggctcgggc atggcggggg acactgccag
    30841 cgggccggcg gcaaggttcc cggcatggcg cgcggggagc gggtcagtga agatcttcag
    30901 agtatgttgc gaaaccgctg gcttggcgtc tgcggcgtcg atgcccagcg cggcatgagg
    30961 ctggtcccca agcgagagat cctcgagaac ctgtagggtc gcgagaatga ccaatgacca
    31021 agggcccaca accaacgaat ggccagtgac caaggcccaa cgaccagtca aatccgaatg
    31081 acaaaatcga gttgtcagtt ctcagtggtc agttgtcagt ttttatggcc ggggcagagc
    31141 tcaccgagca ctgacaactg attactgact acttccgcgg gtttcattca tcattctggt
    31201 ttcgatattg gggcttgctc tcttacagac tcagcagcat ccccgaatgc ggcggcagcg
    31261 tcgcgcgctc gccggccgtg ccgaagggct ctgcgccggc ccgcgccgcg tccgccagaa
    31321 cgctccactt gcctgaggga aggcggaatt caaccggctc atggtggccg ttgagcagga
    31381 tcagcatttc ttccgccgac tcgcctttca ggccgctgcc atccagatga cagacaacac
    31441 atttcggatg gggcggttca ttgaagctga cgcgttgttc gattgcctgg cgcgttggaa
    31501 gccgtaggac cggatggcgg cggcgtatgg cgatcatgcc gcgatgatat gcccagacat
    31561 ccgcgtacgt tttcttgagc gaccagtcgg tccggttgat ttcatcgggc aggttgtagc
    31621 tgttgtgtga tccccgcttg gtccggcaga actcctggcc gctgtgcatg aaggcgatgc
    31681 cttgtgagca aaacagaatc agagcggcaa gccgcatcat gcgtcggcgg gtttcgtcgg
    31741 tttcgtgggg aacgctcagg gccagcttgt cccatgtggt cagattgtcg tggcactcga
    31801 aatagttgat cgtttcggac gggttcacgg tgttcaagtg gatcgagcct tcgagggcgt
    31861 gccgaaggtc ttcgatatgt tcacctgtct ggatgaatcc gggctcggtg ccgttgacgt
    31921 cgcctttgat cgcgttgcga atgccgtcgt taaaggcgcc gatgccggtg ttctgaagac
    31981 gccagcggtc cgtcaaggga cgaagcggtg ttgggccggc ggcccagggt tcgccgtaaa
    32041 tcaggattcc cggcctgatg gcctccaact cggtcttgat ccgcaacatc gtcttgcgat
    32101 cgatcaggcc catcaggtcg aaccgaaagc cgtccacgcg gtattcgcga acccagtact
    32161 tgagcgaatc gagcaggaac cgccgagcca ttgggctttc gctgcggaac tcgttgccgc
    32221 agcccgagcc gttggagaat cgcccgtggc aggtcatgcg gtagtagtag tcgggcacca
    32281 gccgctcgaa gctcgcgcac ggcgacgtgt ggttgtagac cacgtcgagg atcacgccga
    32341 ttcctcgctc gtggaaggcc tgaatggcgc gtttgagttc gatgatgcgt gcggttcctt
    32401 ggcgagacgt ggcgtaccag ccgtcgggtg agttgaaata agacggcatg tagccccagt
    32461 cgtacgggtc ggcatccgtc tcgtcattgt cgaaatccgc aacgggcata agctgcacgt
    32521 gcgtgacgcc gagttcgcag aggtggtcga gccccgtgca cacggtcggg tcgtccggca
    32581 gatgggtgcc gcattccgtc aggccgaggt acttgccctt gtaacgcacg cccgaaccgg
    32641 ccgcgatggt gaaatcgcga acatgcattt catagatgac cgcgtcggcg gggtgggcca
    32701 actccggcga ctcgtggtta cgaaagccgg gtgggtcggt ttccaggagg tccacgatca
    32761 gtgatcgcgg gctgcggccc tgagtgcagg tggcgtagat atctgtgatt tcacgagccg
    32821 gatcaaagcc cggaccttcc agcacgtatg cgtaatactt gtttcgcaga ttacctggga
    32881 tggtgacttc ccagatgccg taagaacaag gctgcatgtc gtgcaggcgc cggttgtgat
    32941 cgccgctggg ggaatcggcc acaactaccc gcacggccgc tgccgtcggg gcaaaaacgc
    33001 ggaaggttgt ggcctcgggc atccaggagg ctcccagcac ggtgcttggg tcatagaaca
    33061 gagcggggtc ttgcagcagc ccgcccatgc gaagcggcgt ccggccgaag acctcggagt
    33121 gaatcaccca ggagcagccg cggaaatcga cgggacgctc ggtttcaaga aggtagcggg
    33181 aggacctgcc gcggagcggt cggcgagcct gtgtcgaacg aatgccgatg acccggccgt
    33241 cgtctccgat gagccggaaa cgctccggtc gacactcggc aacccgcatt cttcgccgaa
    33301 accgcaggcc gatcagacat tcggcatcca gtgttgccag caccatctcc ggacacattc
    33361 gcttggtcct cggcaggcgc gtcgtaagcc tcatgatgag tcgccgggga gtcacatgcg
    33421 ttgccttggt ctctcgccgt tgtcacacgc ctcgcggctc ggccctgctg ttggagagca
    33481 gccgttcgat ggtagaatgc cggtgggccg ggtcgcgcga tcctgacggc gatcttcctc
    33541 tgcttcgtcg atcagcggac gttcgaccgc atgtgtcccg ggtgatatga tatgcccccc
    33601 acgagggaag acaaactgat gcccattgaa caggcggagc tggtggaccg cttgcgatgg
    33661 ttcgtccagc ttcgatggat ctttggggtg gtcgctcttg ccgccgcgac gttgttattg
    33721 cggatacctt tggggcgggt tcatgccccg atgctcgtgg gtatcagcgt cgcgatcttg
    33781 tgctacaact ggctgttctg gttcctcgaa gggtggctga gccgtcaccg accggaagcc
    33841 cacgcccggt atgccgaggt tgcggcgtcc ttgcagatcg tcctcgacct ggtcgccctg
    33901 actttcgtgc ttcacgccag cggcggggtt gggaacccct tctgcctgtt ttacgtcttc
    33961 cacatggtga tcgccactct gcttctgcgg atgcggcacg tcttcctgct ggcgggtctg
    34021 gcgatctttc tgttcacgtc gctggcgctg ctggagatgc gcggctgggt gccgcatcac
    34081 agcctgttcg ggctggagga ggcatacaag agcccgaggt atgttgcgat tactcttgtc
    34141 gcgtttgcca gcgcgatgct gaccgccgtg tacatcggaa cgtcgattgc cgggcggctt
    34201 cgcgagcggg agcacgagat cctcaggctg gaacggcaac tcgcggacta cgcacgggag
    34261 cttgaacgca ccaaccagga gctggtcagg gcagatgagg agaagacgcg gtatttccgg
    34321 aaggtgtctc acgacctgaa gtcgccgctg gccgcgcagc agagcctgct tcgggcgctg
    34381 ctgattcaga tggagaagtc ggatccgcaa tcggccgcac gtgtggagag ggccattgct
    34441 cggggagacg agctgttggc tctgctcaac gacctcttgc tgctgtccca agccaaggat
    34501 gtggttcgcg gcccgcggca cgagtggatc gatccgggtg atcgtcttcg ggccgtgatc
    34561 gatgcccagg cgctggccgc gaaggaaaaa ggattggact tctttgagga gatggaaacg
    34621 cccttgccgg cgatttgcgc cgagcctggg acgcttctta cgctggccga gaacttgctg
    34681 tcgaacgcga ttaagtatac cccaaaaggc gggagtgtaa cgttttccct gttcagcagg
    34741 gatgagcatc tgatcatggt ggtgaaagac accgggatcg gcgtggctaa ggaggacctg
    34801 ccgaaaatcg gccggcagtt ctttcgtacc agccaagccc gtgagtcggg ctcgccgggt
    34861 accggtctgg ggctggcgat cgtgcggtcg atggtcgagg ccatgcacgg ccgcttcgat
    34921 ttccagagcg aactgggcgc cggcacaacg gtgaccgttg cgttgccgct gtcgcatcct
    34981 gaggcgctga gggaagtcga gccgttctgc cgccttgagg agcgtcgcgc cgatgagggg
    35041 acagtcgcgt cgcgagaacg agtgtgatgc tcgcggccgg cgttttttct ctccaagcga
    35101 gtcatcgtga agactgttcg actgaccatg cttgattttc gcgaacccgc ggcgcgctgg
    35161 gggatcatgt tcttgttcag cgccgttgtg ggggttctgg cggggttggc ggcggcggga
    35221 ctggactacg ggctgcgaga aggaacgcgg ctgctcatcg gccggttcac acacattggc
    35281 ggagcccagg ttttcgcttt tcgatggggc attctgttga tgcccgcggt cggcgctctg
    35341 gtgagcagcc tgttcgtcct tcttctatgc cggaaggatt ccgggcacta cggaaccgac
    35401 caggtcatcg ccgcttttca tcagcacggc ggacatcttt ctctctgggc accggtcgtc
    35461 aaagcccttg cggcgattgg tgtgatatcc tgcgggggct cggcgggtcc ggaaggtcct
    35521 atcgccgttc tcggagcggg catcgggtcg aagttcgcga ggctcttgtc gctggcgccg
    35581 cgttcccggc gaatcctgct gattgccggc tgcggcggcg gggtgggggc catttttcag
    35641 tgcccgttgg gcggggcact gtttgcggtg agcgttctct atcgtgagcc ggagttcgag
    35701 gcggaatgca ttgttccggc cttcgtctcc tctgttttga gctactcaac gttcatgtat
    35761 tttcccggct ttgggcatca tctgttgcag ggcgtaaact ctctgggatt tgcgtccccg
    35821 gccgaattgc tggcttacgc tgttttgggc ttgatatgcg gcctgctggc cgggttcttc
    35881 tacatttgcc tgcggatggt cgagtcacga cggttgcttt ccgcccgtct gtccgcatgg
    35941 ttgttgccga ccgtcggcgg gttgctggtg ggattgactg cctgtgtgct gcctcagatc
    36001 atggacagcc gttaccaggt cctgcaaaac actctcgacg gcaaaatctt tctgggcgat
    36061 tccgtggccg ggcgctcggc ctgggaatgg gtcgcgttct tcgggttcct ggccatgctg
    36121 aagtgcctgg cgacggcgtt gaccgtgggt tcgggcagcg ccggcggctt gctgggaccg
    36181 agcgtcttca tcggcggcgc cgcaggggcc tttctgggtg cgctgctcga ggcgtgttgg
    36241 ccgggacatt ttccacccgg ttccccgctc aggcaggccc tggtacctgt cggcatggca
    36301 ggcgtgttgt cggcgtcgat gcggatcccg ctggcggcga ccgtgatggt tactgaaatg
    36361 accggcagtt acggcctgat tgtcccgctg atggtgacct gtgtgacgtc ctatgtgctt
    36421 ggccgccggt ggggtctcaa cccgtcgcaa gttcgctggt cggccgaatc acccgcccat
    36481 gccggcgacc tgctcggacg catgctcgaa tctctgcaag tgcagcatct catggaccgt
    36541 cactggcggt acacggtaac accttcgacg acgttgggcg agatgatccg gcaaatcggc
    36601 agcggctcgc ggccggtatt cgccgtgaga gaagacggac gattcgtcgg ggtgatctcc
    36661 gtacaggaca tcagccgggc gatctccgag ccgggtatct cagagatcgt catcgcggcc
    36721 gacatcatga gcgggcacga caccacggtt acccctgagc agagcattgc cgaagttctt
    36781 gaggtgttcc agaaaacgga tctcgacgtg ttgccggtga tctccgacga ttctgaacgc
    36841 cggcatctcg gaatgctcaa caggcgggcg attcacgagg cggtccgcag ccgcctggag
    36901 cggattcgcg aacacgcgcg gcgcgagcac gcgggcatcg ccgcgatcga gcaggacgag
    36961 cagttgttcc agttgatgct gggggtctcg ggttctcgtc cggatgtctt cacccgcatg
    37021 cccgtgccca acgagattgt gggggtatcc atcaggcaag cggcgtttca acgtcgctac
    37081 ggagctcagg tggttgccat tcagaaccag gatggctcgc tccagtgtcc gccggacatc
    37141 gatgcgcctt tgcgcgaaga ccagattctg gtggtgattg tctccgacgt ccgacaagct
    37201 gaacacggca ctgtcgcggg gccggaaatg tcgagccatc tgtagatcac cctgccttgc
    37261 ggcctgcctg ccagactcca gtacgacggg tggctgtgtg ctgaaatcgc cgtgtgaatt
    37321 cctgccagat ttgccggcat gtcattggct taagtggttg tttctcaatt gattagagca
    37381 gaagaacagg ggtgctttgg ctgattatgg aagaaatctt tcaaagacag gccaagcccc
    37441 gcttgattcc tctcacaact tgtcgataat cggcttaggt ttggtcagat ccggaggact
    37501 catgaccgga gacgagcgac gacaacagtt atcggaagtc ttggcgcgcc gcggttacgc
    37561 tgatctgtcg ctattggtca gcgagctggg tgtctcagaa tcgacggttc ggcgcgattt
    37621 gtcgttgctg gaagaggagg gtatcgttcg ccggacgcac ggcggagcgg tgttcgtcag
    37681 cgatcggttc gtggcgctga attatgcggc ccgggaagtg acggcggttc cggagaagga
    37741 ggcaatcggc cgtgtggccg ccggcttgtt gcaggacggt cagacgattc tccttgacgg
    37801 gggaacgacg acctttcagg tggcccgaaa tctgttgtcg cgaacgatgc aggtggtgac
    37861 caactcgctg ccaatcgcga atctgctgca cagtgcgccg aatattgaat tgattttcat
    37921 cggcggttac atctatccgc gcacgggtgt ggcgctgggg ccgtacgcac aacagatgct
    37981 gtcctctctg catgtggatg cggccgtcat gggcgtggcg gggatcacgg aggatgcgct
    38041 ctacaatgcc aatgtgctga tggttgaggc cgaattgcag atgatgcgtt cggccggtga
    38101 ggtgatactg gttgccgaca gcggcaagtt cggcaagcgg gcgttggtga gactgggcgg
    38161 ttgggaaatg gttgaccgag tggtttgtga cagcgcgctg gaaggccggt ggcaggatgc
    38221 ggtgcgcaac gccggggccg aactgattct ggccggcgtg gaagaggcga cgggtgtcgg
    38281 agcaaggtcc gccggcgcgg gaggtcaggg atgagtacgg cgatggtggg cggcaggctt
    38341 gatcgctcgg tggtcgaggg cgtggttcgg gatatcgtcc ggcagcggct gggtctgggg
    38401 cccgggaggg cgggtgccgc caaaccgaag ctcgttgtga acatctcggc gcggcacatg
    38461 cacgtgacgc agaagacgct ggagattctc ttcgggcctg gggcgcagct tacggtgatg
    38521 cggccgcttt atcaggaagg gtatttcgca agcgagcaga cggtgacgat catcggacct
    38581 cggcaccgca cgatcacgaa tctgcgaatc ctggggccgg tgcggcctga agatcagatc
    38641 gagctggcct tcaccgatgg catcgcgatg ggtctggatc tgcccgttcg tatgagcggg
    38701 gacatcaagg gtacacccgg ttgcatcgtg ctcggaccca aggggcatat cgagctcaaa
    38761 gagggcgtga tccgggctca gcggcacgtc cacatgcatc cgacggatgc ggagtactac
    38821 ggggtcaaaa ccggcgactt catgcggctg agggttcatg ggagttgtcc aacgacgttc
    38881 gagggcatta tctgtcgcgt gagcaaggat gtgaagctcg aggtgcacct ggacaccgat
    38941 gaaggcaacg cgtgcgacct ggcgaacgcg gcgagagttg aactggagag aatgtaagcc
    39001 caagctgata gctgacggct gatagctgat cgcttttttg aggagtccac ttatggcact
    39061 tgaagcactt ggaatgatcg agacgaaggg tctcgtgccc ctgatcgagg cgtcggacgc
    39121 gatgatgaag gcggccaacg tccgactggt ggggtatgag aaggtcggtc gtgggttggt
    39181 gaccgcgttc atccgcggtg atgtggcggc agtcaagtcg gccacggatg ccggctcggc
    39241 ggcggcgagc aaggtcggcg aggtggtcag cgttcatgtg atcccccggc cgcacgacga
    39301 actggcaggc atgttgcccg ccgagagaaa cagggccaaa taaagggaac gggatcatac
    39361 ggggatgacc cgcgccggac gagcgatcgg ttcagggttg gcgcggggta acggagtatt
    39421 caatcgatga gcgatgcaat cggaatggta gagacgatgg gctacaccgg cctggtggaa
    39481 gcctcggatg cgatgtgcaa ggctgccaac gtcgagctgg tcaagagcgt tcagatcggc
    39541 ggaggcctgg tgacgacgat cgtgcgcggc gacgtcggca gcgtaaaggc cgcggtggat
    39601 gccggcgccg aggcggccaa ccgggtcggt gagctgatga gtgcccacgt catcgcccgg
    39661 ccgcatgaag cgctggcgga cgtgtacttc aagtaggcgt tgcgtggcca tgacggcgat
    39721 gacgcaggcc tctgtcacaa cagccggagt gtgtcgtgat cgtattggtt gccaatatcg
    39781 gtagcacgtc gttcaagttt cgcctgttcg atatggacgg cgagcgggag ctggcccgcg
    39841 gaggcgccga gcggatcggc tcggacaacg cggccgtcaa ggtgggcgac aagacgtggg
    39901 cggggcggat cgccgaccac ggccaggcca tcgaccactg cctgaggttg ctgcccgacc
    39961 cgaacgtcaa gctcgatgcc atcggcttca aggcggtaca cggcgggacg acttcggggg
    40021 cggttcgcgt cacggaagag gtcatccgaa cgatgaccga gttcttcgac atcgcgccgg
    40081 cgcacaaccc gccgtatgtc gccgcgatga aggcctttgc cgcacggctg ccggacgtgc
    40141 cgcaggtcgc ggcgttcgag acggccttcc accagacgat ccccccgtcg cgcaccacct
    40201 atggggtgcc gtgggagtgg acgcaggccg gcgtgcggcg gtacgggttt cacggggcga
    40261 gtcatcggta tatcgccgtg cggaccgctg agctgatggg tcgcaaggat ctgcgcgtga
    40321 tttcttgtca tgtgggcggc agcagctcga tctgtgcgat cgagaacggc aggagcgtgg
    40381 ccaacagctt cgcgacgacg cctcaatcgg ggctgattca caataaccgg gtgggcgact
    40441 tcgacccgta cgcgctgctc aaagtattgc cggcgttcaa gctgaccttt gagcaggctt
    40501 tgcaggagat ggctaagaaa ggcggtttgc tcggcatcag cggcgtgagc aatgatcgtc
    40561 gcgacatcga gcaggcggcg gccgagggca acgaacgggc gaggttggca cttgatgttc
    40621 tcgtcgaggg cgtgcggcat tacgtcgggg cttatctggc ggtgctcaac ggggcggatc
    40681 tgatcgtctt caccggcggc atcggcgaaa acagcgcctc ttttcgtgag gccgtttgtg
    40741 cccgaatgga ttacgcgggg atcaagctcg attcgcggaa aaacgagacc cgcggcagag
    40801 aggaaacgat ttcagcggat gactcgcgcg tgcgaatcat ggtgattccg acaaacgagg
    40861 aactgatcgt ggcccggcag acgcgggacg tactggcggg cggcaactga ggatggcaga
    40921 gacggccggg cgatgtggcc tgtgccgggt tgattgagga gtaatcggaa atggcccagc
    40981 aggcattggg attgattgag actcgcggca tggtggcgct cgttgaggcg acggatgcgg
    41041 ccctgaaggc ggcgaacgtg acgtatcgcg gctggaagca ggtcgggtcc ggcctggtga
    41101 cggtggttct cgaaggcgac gtggcggcgg tcaaggcggc agtcgacgca ggtgcggcct
    41161 cggcggcgaa ggtcggcgag gtggtcagcg tggatgtgat cgctcgcccc cacggcgaca
    41221 tcggcaagat attgccgaac aagtgatgcc gaccggccgg gctgtccccc gtcgttgtcg
    41281 cgggaacgct gccgtcagcg gggctccgat gattgacgga cgggggtttc gcccacgttc
    41341 ggggctgaat cacgaacgtc cggtccgcag acagggagac gacatgttca tcggcaaagt
    41401 gactggaacc gttgtggcat cgcagaaggt caagagcgtg attggtagga agctcttgct
    41461 tgtccaggcg atgaatgtca aagctgacgc gccgggggcg ctggaaacaa ccggccggac
    41521 ggccgtagct attgacacgg tcggagcggg cgagggcgag ttggtgctgg tcactcaggg
    41581 cagttcggcc cggctgaccg aaacgaccgg cgacgtgccc acggacgcgg tgatcatcgg
    41641 catcatcgac gcgatacaga tgaaggacca ggagatctac aagaagcagg gctgagcggc
    41701 cgtcgtcagc cgcggcaatg gagcgctaaa gcgtattccg taaggcggct ggggtcttgc
    41761 ggttggagcg atagagatga gcacgacagt gagtgaggcg ttgattcgcg atgtggtcag
    41821 tgaggtgctg agccgactgc aaggcaacgg aagggttgcg acggcgccgc cggttccggc
    41881 aggcggcagg gacggcatct ttcagaccgt cgatgaggcg gtagctgcgg cgaaggtggc
    41941 acagaagcga ttggtggccg agacgctgga gacacgggcc aaggccatca agtgtattcg
    42001 ccgcatctgc ctcgaacaga aggaggaact cgcccggatc gagtacgacg agacgagaat
    42061 cggccggatg gaccacaaga tcgagaagct gatcgtggtc ggcgagaagg tgccggggat
    42121 ggagatgctt acctccgagg cggtcagcgg cgaccacggc ctgacggtca ccgagcacgc
    42181 cccgtggggt gtgctgggcg ttattacgcc cgtgacacac tcgattccga cgctcggctg
    42241 caatgccatc atgatgatcg ccgccggcaa cacgctggtc tgtaacccgc acccgtcggg
    42301 ggccaggtgt gctgccgtcg ccacgcagcg gtggaaccgg gcgatccgcg aggcgaccgg
    42361 cctggataac ctgatctgcc tgatcgagaa accgacgctg cagacggccg accagatctt
    42421 caagcatccg gatatcgcca tgctggtggt gaccggcggt ccgggtgtgg tcgcagccgc
    42481 gatggccagc aacaagaagg caatcgttgc cggcccgggc aacccgccgg tggtggtgga
    42541 ctgcacggcc gacatcgaga aggccgcggc gggcatcatc ttcgggcacg cctacgataa
    42601 caacctgctg tgcatcggcg agaaggaggt catcgtcgtc gaggcggtgg ctgacaagct
    42661 gctcgaggct ttctcgcggc acggcgggta ccgcctcaac gccgagcaga tccgggcatt
    42721 cacggcgatg gcgttgcaca aggacgagaa gagcggccac tggttgccgg acaagaagtt
    42781 cgtgggcaaa gatccgcagg ttctggccga tgcgatcggc atcaggctgc cgcagggcac
    42841 gcagtgcctc tatggcgagg tggatgagca cagcccgtgg ctgccatgcg agcagatgat
    42901 gccgttcctg cccgtcgtcc gggccaagag catcgatcag gcgatcgcca tggccgttga
    42961 gtacgagcac ggcttcggcc atacggccat catctggtca cgcaacgtcg agaacatgac
    43021 caagatgggt aaggcggtgg acaccacgat attcgtcaag aacggcgcgt gcatggcttc
    43081 gctgggtgcc ggcggcgagg gttttgccag cttcagcatt gccacgccga ctggtgaggg
    43141 catcacgagc ccgatgacct tcacgcgcta tcgccggtgc gtgatggtgg acaacctgcg
    43201 cattctgtag ggcggagaaa atgatactgg ggcgtgtggt cggcaatgca gtctcgacgg
    43261 tcaagcacaa gtcattgggc ggcgcccgga tgctggtggt tgagccgatc caggccgcct
    43321 cgctcgaccc ggtgctggcc atcgatcagc tcggggcggg ggcaggcgac atcgtggtgc
    43381 tgaccagcga cggcatttac acgcgcgagg tactcaacga caagacctcg ccggcccgct
    43441 ggacggtgat gtgcatcgcc gacgacggcc agtatgtgat ggagcacctg gaagaatgag
    43501 ccggccggtc gtgaccgcga ttctgcttga ggaccttcgt cgggagggca aggagatccg
    43561 gttgcccgag ggggccctgg tgacgccggc cgcccgcgac tggctgaaag agcattcgct
    43621 gccggtcacg tgggagaagg ctcccgccgg ggcaccgggc aggctggcgg tggtcatgga
    43681 cccgtcgctg tccgagttgc ggatgatgcg ggctatgctt gatcggggcg gacagctggc
    43741 cgaggtcatc gagccggccg gcgggcgaag cggcatgttg gcggcgaccc ggcggctgtg
    43801 cggcaaaatc gcccgtggcg aggtggccaa gggcgtggtg tttgccgccg atgggccggt
    43861 gccggtgtgc gtagccaaca agcacaacgg cgtgcgggcg gcgttgggcg tcaacatccc
    43921 gatgaccgag gaggcctgcc gctcgctggg gattaacgtc ctggtgctgg agtacccgac
    43981 gctgacgccg taccagatga aacagatgat tgaccggctg ctggccggag cctcaccgcc
    44041 ggcggagatg ttggcggaga ttgaggccgt cgaacagggg ggcggacgtg cggatcggtg
    44101 acgtggttgg acggctgacg ctgagcaggt gtgatccgaa gatgatcgga ggtcgctttc
    44161 tgatcgtcca gccgcacgat cccaagtcgc tccgcgaaaa ccgtccgggc aagggcgaag
    44221 tggtcgtcgt gtacgaccaa ctaggcggcc gcgtgggcga tcgggtcggt ttcacggaag
    44281 gccgggaagc cgcgatgccg ttctatcccg accgcgtggc tttggatgct tatctggtat
    44341 gcctgctcga ctgcgtgacc tacacggcgt gagcggatac catacggtga actgtcatgg
    44401 ttaacgagtt caagctcaag caggaaatct gcgaaatcgg ccacaagctc tatgaaaagg
    44461 gcttcgtggc ggccaacgac ggcaatatct cctaccgtgt cagcgagaac cacgtgttgt
    44521 gcacgccgac gttggtcagc aagggggccc tcaagcccga tgacatctgc ctggtcgaca
    44581 tgaccggcaa acagcttgcg ggcaagaaga agcggaccag cgaggtgatg ctgcacttgg
    44641 agatctacaa ggcgaatcca aaggtgaagg cggtggttca ttcgcatccg ccttacgcga
    44701 ccgccttttc ggtggcgggc gaggagattc cgacctgtat tctcccggag gtcgaggttt
    44761 ttctgggacc gattccgacg gccgtgtacg agacacccgg ggcccaggca tttgcccgga
    44821 cgattctccc gtttgtcaac accgcgaaga tcgttgtgct caagaatcac ggcacggttt
    44881 cctggggcga gacgcttcag caagcctact ggtggaccga gattctggac gcctattgcc
    44941 ggattctgct gatcgccaag caggtgggac gggtcgagcg gatcagtccc ccgaaggtgg
    45001 aagagcttct ggatctcaag gagcgcttcg gcatcaccga cgacccgcgg cggctttata
    45061 acgcggatct ttgcgtgaat atcgacttcg gccggggcta ctcggagccc ggctcaaccc
    45121 tcatgaagaa gcccgagggg ccgatgagac ctccggaacc ggttcaagct cccgccgtga
    45181 gtaacgaaga ggtcgagcgt gtggtgcagg agatcaccga tcggattatg agtgctctgg
    45241 gatgagagaa gtcccgcaat cgcgaggtgc ctgagatgga cgctgatgtc tgtcatgctc
    45301 gaatcaaggg aatgttcgcc gtcgcggtgg cttggctgtt cgtcctgtgc ggctgtcacc
    45361 agacgcgcta caactgggtc ttgcacgacg tcgatgtgac gctgccggaa atgaccgccg
    45421 ccggaagggt gaccgaacga gcgggcacac cggcggaatt gctggtcgta cggcccggcg
    45481 gcggcaagac catgcttgtc acagcgttga tcaccgatga tcccgagaag atcgacgacg
    45541 aaaaagcgga aacgttcgtt tgcgtccttg atggaccgcc gctcatcggc cggaagaccg
    45601 ccgtcgatgt tgaccggtgc cggctcatac tcaacgaggt gttccggccc tgcaggcggc
    45661 cttatcaagg cgcggagggt agcgtgcgga tcacgtcggt tcgcgggggg aaggtgacgg
    45721 cggagatcgt ctttcgcaac gtgcttcgct ccgcgcgaga tgattcgtac atcgtccggg
    45781 ccacaaaaac cttcaggccc gccgcgcccg atgacgtccg gcttcgccag gcgggcattc
    45841 agtacgaacc aacgccggct cccgggacgc tgccctgatg tcgtcggtcg ccttggagga
    45901 aataaagcag agaggcgaag atcccatgcc gtcttgtggg agagttagct gtctatggta
    45961 gccaagagtc tcgatatcaa gcttgccaga atcaaggccg atccggggtg caaggagttt
    46021 attctggccg acgcaaagga tgcggacatg gcctttggca ttgccgcgcc cggcaagtcg
    46081 cctgaacact attcgcagga gggcaagttc cgctcactcc cggagtttcg cgagtgtatt
    46141 cgccagatcg tccggcaggg gctggtggac attatgttga tgtccgccca caccaattca
    46201 attctgacca tccatgaacg tttgttcgac aactcgcacg tgacgcctgc cgccagggcg
    46261 aatgatacca ccgacatcca cctggcgcgc gggtccatct atgcttccga gccttcccga
    46321 ccattccgca cggtgactat cgaccagatt cagtgcggca aggtagagtg tcaaccgacg
    46381 gaacgggtga tcggcgccaa ccttggtctg tacagtgtga ccttcaacaa cgatctggag
    46441 ctggaccggc tcacgctgga gcagtaccgg gaatttcgca tcgaggccga gcgcaagggt
    46501 ttcaggcatt ttctggaggt cttcgatccg aacgccccga ccaacccggt cccgccggac
    46561 aaactcgggg gcttcgtcaa cgatatgatt gtccgatgcc tggccggtgt cccggagggc
    46621 ggtcggccgt tgttcctcaa gatggtctat cacggaccga agtggacaga agaattggct
    46681 agctacgatc cgcaactggt gatcggcatc cttggcggtt cggcgggcac gacctacgac
    46741 gccttcaaga tgatcgccga ggcccagaag cacggggcca gggtcgcctt gtacggccgc
    46801 aagatcaaca acgccgagaa ccaactggct ttcatcgaga tgctgcgtcg catcgtagac
    46861 agccagatct cgcccgaaga agcggtcagg gcgtatcacg gcgtgttgca gggtctgggc
    46921 atcaaaccat accggtcgtt ggaagacgat atgaagctga catcgcaggt gatgagctat
    46981 gcggggggtg gtcgcgttca ggtgcccgcg tcgccggcgg catcccctcc tcgcaagcct
    47041 tcggccgctg cccggcctgc ttcggccaag cccgtcacca agccggctca gggatatgtt
    47101 gccgccgacg cccctgactt ttccaagatg acggccaagg agcgggcggc ctaccatcgt
    47161 gccagaatcg ccgggatttg aggccggtca tcgaaggctt gggtctcggg gagcagccag
    47221 gtataattcc gttgtcccta tggagcatcg accatgcggt atctcaatag cacactgtac
    47281 agcctggctg ccccactggc cgcgttgccg ctgcccctga tgcggatgca gtttgacaat
    47341 atcggcgact acatccgcaa catggagccg cagctacggc agatcatcgc cgaggtgatc
    47401 gttgcccaga tatccacgat tgccgaggcg ttgatcgtag ccctcacccg gatcttcttt
    47461 ggtatcgggg gttagcccct tacggtcgcg gtcctgaata caccgtaatg cctccctgcc
    47521 gaaggcgggc cgccattttg tcggccgcaa cccaaacgtt ctgcgatcca gaaacatgcg
    47581 cgcaagccgc acacatatcc ggccgtaagg ttctaaggcg gaccgccttt ttgcgggccg
    47641 caacccaagc gtaccgcgat ccggaaacat gcgcgcaaaa cgcgcaccag tcgcgacgga
    47701 agagactaaa tcgactgaga agatgctcaa ggtggaaggc cggaccaagc ccttcctcaa
    47761 tgggaataca aagcgatcag gacgcaccac ctcaaacctc gccaattgag gccggcggca
    47821 ttcccgggct attccctcgc caccggcagg tcattctttc gccttgtcgg ttgttgccgg
    47881 agcaccatga gacgcagtcg ccgggcgagt ggtcgcgatg ccgggcaagg ccacgttggc
    47941 aagttcgtcg gcatccacga agacatgcca gcgctgtcga ggaacacctt caaacgcagt
    48001 ctggaaacgc acggcgtcag gagactcatt ttcgattgct cgaatcagaa tgaaaaccac
    48061 ccggtccggg tactttcgcg caagcgtgcc gtacgcttcc ggatccttct cgcccgaatc
    48121 gccgacaagg atgaactgcc gttgcggaaa cgactcgatg accggctcga cgacccccag
    48181 cttgtactca agcgggtcgg cgaagagcga caggaatcgc gagtccttcc aatagaaggt
    48241 tttgagatga aacgttacgt cggggaagcc gtgctcgttc gtgaactccg ttaacggtgc
    48301 atacaattgc cagggactgg cggaaatgaa atggatgatc gcgccctgct gcgcccatcg
    48361 ttggtacagc ttcgccatgc cgggtaccgg ccgatacggc ttgaggaacg tgttctcgat
    48421 cacctcttca agatcgctca cctgcgtgat tttgactgtg tcgtcaatgt cggagatcac
    48481 ggagaggccc gcgggcgggg ccagcacggc cctggccgaa aagcgccgct tgtccgattt
    48541 gggcgtcacc gcatcgaact cgacccattg ccggccatac ttgtccggcc gggacaaccg
    48601 gtcggcctcc tcaagcgaca gcctgacgct gccggtgagg tgcccgttct gttccgatgg
    48661 gccgacggtt gccgttcggt cgccgattcg gagcgtcaga gtcttgccgc tctcgttgtc
    48721 caccaggaaa gccgcagctc gccggttgaa gagcctctcg ccggcatgtt cgtcatccac
    48781 atccagcgcc gctcgcaggg ccgccaacac gaccatacgg cggatggagt cggtttcggg
    48841 ctcgaagatc cagccatgga tcggcacaac ccactgcgcg gcgacggtat cgaggcgggc
    48901 agcagtgttg aagaagataa cctcctcgtc cggagcaatg ccggattgtg ccaccgacac
    48961 gggcgcaagc ggaagaaggc cgcacacgga caagcacgtg actcggcacc gtgtccatag
    49021 tcgcgctcgg acgggcgaga cataggtacg ttttccggca tcttcagcac gtgctgccat
    49081 gtcaacagcg tcgccgccga tgaccctggc gtcaaggcct cgccgcaagg tggaaggtcg
    49141 aatgaatcga agcgccgcga cgtcaagggc tttaagagga tggtcgagga gaaagaggaa
    49201 ccacgcgcat cgctcagcag gcgggtacca tatcactggt aggggtgcct gaaccgatga
    49261 atgaaggcgt attcgttttc ccagccgccg ttgtttccgg cataggtgta tgggaatcgc
    49321 agttcgttga agagatcgac gccctgcagc ttgaacacgt taccagcgag gcccttcttg
    49381 ttgccgtagt aaggataccg tttcgattcc gcatggccgt cgaggtagcc gaaattggcc
    49441 acggcgccgg tcgtgcgggg atcatgccgc aaggccagat acgagtcggc cgcccgggaa
    49501 tcccccacga aaccgtggtt tgtgttgttc cacgggtgct cctcgataag catggtcatt
    49561 acggcggggc tccaggtgat cctcgtcccg accggaatca tttggatcgg cgtaatcatc
    49621 gccccttcca tcacctggaa atcggtcacg tacgtggact cctgaatctg ctcgacagcc
    49681 ttgaagccga ggatgccgtt catcgtgtag ctgaatcggc cgttgccccc gccgccggca
    49741 ggattctctg cgtcgggcat gcctttcaag tccgtgggac acaagtagac ctgctcgtcg
    49801 tttacgcatc gccacagcga accttgtcga ggcactgtgg ctgcaacgtg gacctacaag
    49861 gcctgttgct cattaacgtc ccaggcatgg tcaggcgggc agagcagacc gagccaggaa
    49921 tcgccggatc gccagttcgg ccagcggcgt ccggccgggt tcgggggtct ggacgaagcc
    49981 tgataggaga ccccccaaac agtatttgtg ccaggcaggc gaccattggc ggccgcatag
    50041 acgtgtacgg ctgtggccaa ctgcttgata tgcgtcctgc agaccgttcc ctgtgccaga
    50101 cggcgggccc tgatgagcga cgggaccaga atggccatca acaaagcaat gattgcgaca
    50161 accaccagaa cctcgatcag agtggatgcg gctctgcgac ggcttgcccc catcatggtc
    50221 tcacatgaac caggcactgg agtggacaaa gcggtccgca ttaccctgga cctccctgct
    50281 tcggtgaaag gccccagtca ggctgcgcgc gagtgtgcgt cggctgcaat ctgacgttca
    50341 acagggccga cggtgcgcaa gcagactcag gtaatgaaat gaataggaac ctgtgtctgc
    50401 gcgccgcgct ggggcaagta ctcttcgtcc ttgccgccga gggccggctc tcgttgcccg
    50461 actgactccc gagacgtgcc cgccgatcct ctgtgagata ccagaatgcg gccgcggccc
    50521 tggctcttct taaacgcctc tcagaagccg gcgggacttt cctgcctgca tcggccagtt
    50581 tagcacgaaa tgcccgagcc accaatccaa ccagaagagc ggggtttgat gataggcctc
    50641 ctttgttggc gccgagggcc gatgcagtgc aatgcgtggg cttctcgcgg tcgtctttgg
    50701 ggtgcgggtg cgaagcttcc gtcaggttcg atgttacgag tcgcgacgtt ttggccttgg
    50761 aatcatccga cgctcggctg aggactattc aaacgtctgg aacgtattgt aaaggaaggt
    50821 ctcagctcga cttttgccct ttttccagcc tctacgcggc cggggcaaga gcgtacatca
    50881 gcatggcctt gatcggccgg gatagtttcg aaaagacccg gtgattcccg tgggctgcaa
    50941 aaacccaatc ggtccacaac cagcggcgta cggcggtgat cgcgtcggag aaggtgcggt
    51001 ccgtcaggct gggccagttg cagctcttcg gcagctcgac gccccgctca tcggccacct
    51061 tggaaaacag tagacaaatg atgtgctcga tgcagtgtac cctttcggcc aaggacgtgg
    51121 acggggttgg gaggttgggc gaggcgttcg gggtggaacg gatgacggga aatcggccac
    51181 ggttttcctt aagtgtttaa tcgataacga cttaagatct cggttgggtt cgtttggcgc
    51241 ggggggtggt cggcggtcgg tgtccggctt ttggcgcgcg ggggtcgctt cgggatctct
    51301 tgcggtctgg ccatgtcgcc agccgaggat cagcggtcgg ccggagggga cggagaaggg
    51361 gttgcgcccg ggcaggaggt catcgcttgc ggcgttgggt tcgtttcgtt aggcactcgg
    51421 caggtcggcg ccgtgcttgg tcgatgcgac ttggcggggc tgtgacccgc tggtccccga
    51481 ttcccatctg ggacccttct gccgacgatt ccgatcgtta ttcattcctg aatgcggaaa
    51541 ggattccgca ggggatgcgg cccggaaagg attccgggc
//
LOCUS       DAOTPW010000003        51241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_735_length_51241_cov_5.216563, whole genome shotgun sequence.
ACCESSION   DAOTPW010000003 DAOTPW010000000
VERSION     DAOTPW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 51241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51241
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_735_length_51241_cov_5.216563"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="rplI"
                     /locus_tag="P5316_00460"
     CDS             <1..522
                     /gene="rplI"
                     /locus_tag="P5316_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRR83451.1"
                     /translation="LAGEVIEVSEGYARNYLIPNHLGVEPTKANMKRIEEDKRLAEEE
                     RRRRRAALEEMAGRMTNVEVTISAACNKEGHLYGSVGPREIAAALRDEGHDVDAKQVQ
                     LREPIRQLDSVVVPVLLADDLQVDVKVWVVRETGSIVEEDEEEERRRPEKPGREAHPY
                     DRGTGVDALDLNL"
     gene            479..2002
                     /gene="dnaB"
                     /locus_tag="P5316_00465"
     CDS             479..2002
                     /gene="dnaB"
                     /locus_tag="P5316_00465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRR83452.1"
                     /translation="MTAEPASTLSTSTSDVRPAPGLPVRVPPQDIEAERGLLGSMLLN
                     REAIGDVLPIIGQNSERFYRPDHRKLFNVLIEMYDRGDPIDLVTVRNELERRGLLKEV
                     GDVDYLVQLAESVPSHLHAEHYARLVVDKAMLRDLIGAAGQVIEEAHNHRQEAKEILD
                     RAEQLLFHVTDQRVSEQAEPIRRLLEETFHQIEAREGHYITGLPTGFIELDDMLSGLQ
                     KGEMIIVAARPSMGKTAFGLNVAEYVATRERKPVVFFSMEMSRQAVAQRLLCARARVD
                     SHRLRRGMLSSDDLQRLTEACDGLAEAPLLIDDTPGMSVLELRAKTRRLVKRFAVEAV
                     FVDYLQLMRAPNRTESRQQEVSEISRGLKALARELNIPVVVMAQLNRNPEQRTGNRPR
                     MSDLRESGAIEQDADVIMLLHREEYYQKENPNPEIQGLAEVIVDKQRNGPTGIVKLFF
                     NQRLTRFDNLAAGQEPSYVATAGYDSFAEYQSETSPPDYDEHVGRSLPAADEESAPF"
     gene            complement(2151..3842)
                     /locus_tag="P5316_00470"
     CDS             complement(2151..3842)
                     /locus_tag="P5316_00470"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HRR83453.1"
                     /translation="MAKVKAFALGLDFGTNSARALIVDPANGKEVATAVAGYSSGEAG
                     IILDRRDPNVARQHPDDWLTAMCKCTKDALRKAKAVKGFKPEGIIGIGVDATASTPLP
                     VDATGEPLVRQRRFAKNLNALAWLWKDHTSFAEAAQITAAASKEHPEYLAKCGGTYSS
                     EWFWSKALHCIRVAPEVIQATAAWVEQSDFIPAVITGNKNPAEWKHNICAAGHKAMYH
                     ASWGLPAADFLDRIDPALGDLRRRGTYEKAYSSNERAGMLCPEWAAKMGLPAGIPVAV
                     GSIDAHLGAVGAGVKPGSLAKILGTSSCDMMIHPNTSPLPDIPGLCGIVDGSIVPGYF
                     GLEAGQSAVGDIFNWFVNYFDAGEKAHARLAQEASKLKPGESGLLALDWNNGNRTILV
                     DPRLTGLLVGQTLHTTRAEVYRALIEGSAMGARMIMERLEEYGVKVEEVINCGGIAEK
                     SPLVMQIYADVTGRPMKISRSAQTCALGAAIFGAVVAGSATGGYDSVEAAQARMCGLK
                     PKLYKPDAKRHEVYNQLFKLYRQLHDAFGTTGYRESLHNVMKDLLAVRDRVRGIA"
     gene            complement(4123..7080)
                     /locus_tag="P5316_00475"
     CDS             complement(4123..7080)
                     /locus_tag="P5316_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83454.1"
                     /translation="MRNTILAVAGLALCLPAFGQTNLLVNPGFSDPVGPGACGPERGW
                     RDKTHPTGWQCSDWNRFSRWFAKNEENDWELINLCPGGPNADGYYGALENADKLTAAM
                     YLYQTVAVEPGKTYRLSGVWAAMSRMRADGRWTKMRAHVQLIDGPVTGTVLKDRSVIV
                     GDRYSFPFFGWEEFTPLIATPTTAELTVKIWAEQLAPATWSHMEAIVVDDLVLAEETD
                     CTLQPEIDSPTNDAGAAVGVISTQTGDRSVPRASTQTLTITGLNFTPDQMAVKLKRKG
                     SADIVGTITSSTATSIVADFVLPADMALGPWDIVVTGPGCVPAVAPGALEVYLPAFSN
                     GSFESPAAPACTPTRTPDFPTDWLACDSGHWRHDTIWGDLYVPNPDMRQNFLNLNGYT
                     IGNNPLSWTPTCPADGNNYGTMARLTAGIDTSRSATAFQTFSVTPGQTYTFSGLFAWK
                     TALGIARMGLLDGDSSVRISNLTTSPTNNKILVNVTSGTSLSNWKFAYYSAVANTPRM
                     TAFFFCEQKDVTSTEISLHADALSVKPCVDSFTLNGTITPSQGESGKVVQITAIPGSG
                     LVSSPEPPVVILVGPAAIMATNVQVVGGTSIRCQLDLTNAPVGTYNVVVMQQGCVNDT
                     SLQNAFQVVQPYAVQLNSISPAHATNDGPAVVTLTGTNLEGLNGIKLVRDTGGSEIIG
                     TLGPAGSNQTERTATFDLNDKEMGRYNVVPIVVGGATPLYRAFLVVAAAPSNLSFEIG
                     TNPNDPPGGPCVNGTYDRSRALHWDTVPAEISWMRDTRVYFSAPCPQEGDHYASFTAY
                     DTVVLRAFQTIKASIGQRVDASAYLYNKIADTSMSHPTRLILLDGDENGTELASVEVV
                     SPMATWQQVQVSGTVFSGLATVMVETIGSVGRAVSHVDNVGIALVGGCSPVEVDADND
                     SDVDQADFAAFQVCFAGEGVTTLPDAPYSCRCFDHDRDLDVDLADYMAFERCSTGPGI
                     LLDVHNPPEGCVP"
     gene            complement(7354..8223)
                     /locus_tag="P5316_00480"
     CDS             complement(7354..8223)
                     /locus_tag="P5316_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR83455.1"
                     /translation="MSGTRIQVGTAILVGMLLLVSPAAGGVRYSEQGGIQQIWITADA
                     FTARSMSDGTPDYFPDPKANAPLSGSAYYFGKDVNHAGSWSHSGGYQEDWWVQYEIPK
                     SALPAHFNLTGDWGVWFRSQIADAAEYGGTPGGNPDMFWDSDWLFVNGHPSDLNVSNP
                     TEADWQAALAGRANDDDRVLQNFIWVDPDYIGGVRPNWTWLNTEQPDDRDFLAKTFNL
                     VDDKITFRLYEREAGVYNGRIDVICFARTGHPDKPGFVDYVPTDADFLASVPEPSSLL
                     LLLAGTMFLRRRS"
     gene            complement(8586..9488)
                     /locus_tag="P5316_00485"
     CDS             complement(8586..9488)
                     /locus_tag="P5316_00485"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRR83456.1"
                     /translation="MAKRESNVPAGQRRFGAHMSIAGGVHAAFRRAVEVGCDCFQVFV
                     KNQRQWSAPVLTVEAVRLWEAARSRTGLGPVVAHATYLINLASPDRALWRRSIHSYTD
                     ELRRCERLGIVGLVVHPGSHNGRGEGWGIRRIAAALNVIHARTEGSRTRTVLETTAGQ
                     GTGIGWRFEHLAGIISQTDAPHRIAVCVDTCHLFAAGYDLTTDAGYAETFQAFDRIIG
                     LDRLLCFHMNDSKKPLGSRVDRHEHIDKGRLGRSAFRRLVNDPRFLGRPMILETPKGA
                     DTRGRDFDRVNLRFIRSLLREPDT"
     gene            9884..10642
                     /locus_tag="P5316_00490"
     CDS             9884..10642
                     /locus_tag="P5316_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR83457.1"
                     /translation="MNNPWQYDETIQVGTDYRDENEVRVYDRRMQTLRDVGGEVKDIQ
                     RALLLSSDSIVWEIGTGTGECALALAPAVRHVYASDVSPAMLEYARRKAEQRGVSSVT
                     FEKGGFLSGFRPDHPVDGIITQLALHHLPDFWKSRALDVITGSLRPGRRLYLRDVVFP
                     STTDDYDVFFKTVVDGVRSQAGDEMAQRTIQHIKTEFSTLDWILEGMMIRSGLEIVEK
                     SSTGFVSVYVCERITDGETAKSGNTVPFRPTRFS"
     gene            complement(11021..11518)
                     /gene="nrdR"
                     /locus_tag="P5316_00495"
     CDS             complement(11021..11518)
                     /gene="nrdR"
                     /locus_tag="P5316_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRR83458.1"
                     /translation="MRCPYCKEINQDKVIDSRLSEGGEVIRRRRVCQFCHKRFTTKER
                     VENEVRLLVIKRDGTRVPYDRSKILAGLQRACYKRPVDDDSLAKLVDAVEDAVFKEHD
                     REVSSQTIGAIVAAKLRDVDPIAYVRFASVYRRFADLGELIDEAKSVYDRSRNDVPGQ
                     QSLFE"
     gene            11899..12774
                     /locus_tag="P5316_00500"
     CDS             11899..12774
                     /locus_tag="P5316_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR83459.1"
                     /translation="MASRLIEKTVGGVRLQGFSLAGEETILIAPEMNVAFDIGRAPRE
                     AIGIDHICLSHGHMDHAAGLAYYFSQRHFQGNAPGTVLVHHKLVPAIEDLLGVWGRIE
                     GHVSPGIIVGVDEDEDFQLHRSLVIRPFRVCHPGPTLGFSLVEVRKKLKPEFAALSGR
                     QIAELKRSGRIVEDRLEIPRLAFCSDSAIGSFLDRDHVRNAEVLIFECTFFEPEHLER
                     ARQGRHTHIDDLPQLFERVRSPHVVLMHMSRRTGIGAAKRYLRKLLKPVDLERLTLFM
                     DLPPQRRSRQADEEA"
     gene            complement(12960..14171)
                     /locus_tag="P5316_00505"
     CDS             complement(12960..14171)
                     /locus_tag="P5316_00505"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR83460.1"
                     /translation="MRTIAIANQKGGCGKTTVSINLAACLAREGRRTLLVDMDPQSHC
                     ALGLAVPEDQIEVSIHDVMMHRNNGDRIEITRCIWQITANFDLAPSTTALSEFELIGP
                     TLPDAEQRLGDALVSVSDKYDFALVDCPPHIGLLTLNAFHAASEVIIPVDTGYFSLQG
                     LTKQLETLEAIRSTRQSPLEIRVLANLYDVRTKLGREILNELKRKYGHLMCHTFINFN
                     TKLREGASFGQPITEYDPNSMGYRDFARLARELISSGGPEMVPTAILRQADDLAERAE
                     KLLATSKPLFGKGELSQAGDATPATPEEVQKKIEGIYGVHKTNEGVLFATEAPGATSV
                     AIAGDFNNWQPGATLMERNGDGNSFVALLPLPPGRYRYRYVIDGQWRSDPNNEYVESN
                     PFGELNSVVEV"
     gene            complement(14366..16192)
                     /locus_tag="P5316_00510"
     CDS             complement(14366..16192)
                     /locus_tag="P5316_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR83461.1"
                     /translation="MVKDDTPNLDPLILERNLAALREVDPELAQRLADLPDASGPPPM
                     PARTRDGRLSFRLKQSDGWMTWFGRTSIPGVRAAALLDRFDAGRANVFFPGMGEGSEV
                     EMLLPRLGAHRAIFVWEPDPVNLKLALRLHDLAAAISAKRLVLMTSGLGQLADALYQW
                     LMRNPGHLCPNQMMMFPWQTPAEIAPIRFAVEQAYQRTERDRQTALAQVHHRWRTMPH
                     PQSPAAGDAPAHVLILALHALDETWSTADALSVAAAKLGWHATVADIRTPGDMHSLAR
                     ARRVLESPHGPPSFSILLNVIREQVCDVIPAAIPVVCWLSHRGDVFHRARLGAVAVTS
                     PVLLKRALAAGIDQRRLSVCPLPCLGPVELDCLEKERPLDVLLITDAGPIDAAAIAPR
                     LPGYAQLWKGAADLLATQIETFTDDQAERILSRTEAKARARIDEPQVREEILRNLTCQ
                     LAPSLLCRFLAGQLKASGLSFRIHGPGWTAAFPEHASPPLTTIRQKVDVLRCAKVVIH
                     ADVTGLLTGDALLAAGCGAAVIARRHPSNTLPGGLETLLAPDHEVLTFLTGRELIQAV
                     RRLLEDAACRCDLAAMAVRRCLADHMPEARLETLKAAATSVF"
     gene            complement(16219..16404)
                     /locus_tag="P5316_00515"
     CDS             complement(16219..16404)
                     /locus_tag="P5316_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83462.1"
                     /translation="MPDTTKKDSQQREPKSIWDNDLPAGDSPPRPRGPLIAAAVAYGC
                     WLIFLLVMTILRMTYHA"
     gene            16403..16672
                     /locus_tag="P5316_00520"
     CDS             16403..16672
                     /locus_tag="P5316_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83463.1"
                     /translation="MVTQPVYHRSARRTLAFASHIVRVKVVPPPVDGPSYQHPRRVAR
                     MVGGLVGSCVWALCEVPIASSSLWGAICKPPITGQAAMPLLDKRP"
     gene            complement(16760..17578)
                     /locus_tag="P5316_00525"
     CDS             complement(16760..17578)
                     /locus_tag="P5316_00525"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRR83464.1"
                     /translation="MAIVREVTDPDQALNQLREYLQTLILRSFHESEAFRPLAFVGGT
                     ALRFLHGLPRFSEDLDFSLVTADGYAGREWMSKVKRDLTLGGLNAEVTWNDRKAVHTA
                     WVRLPGILHDAGLAAMPDQKLAIKLEIDTRPPAGARCERRVISRHLTFLVQYYDLPSL
                     LSGKLHAAITRRYAKGRDWYDLMWYLSRQPPVEPNLPLLQNALDQTHGVGRYDAKAWA
                     TLVRDRVRSIDMKRIQEDVRAFLERPLDAALLNNENLLGLLPTEGPTDCTPDPP"
     gene            complement(17590..18231)
                     /locus_tag="P5316_00530"
     CDS             complement(17590..18231)
                     /locus_tag="P5316_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83465.1"
                     /translation="MDYGSLVKLTGTLPCFDLALLVQSFDDRREAIRVQLSRWMKQGK
                     LIGLRRGVYTLPDTYRRAPVTPARLANDLYRPSYLSGLWALGFYDMIPERVVWLTSVT
                     PRVPRRFENPLGVFDYRNIKQDCFLGFSSLRHGGEEILVAKPEKALLDHWHLTPGEWT
                     SERLAEMRYQHVDRVAEDRLREYATCFRSPRLDRAVERWLTLAAEEAKGTVTL"
     gene            complement(18454..21504)
                     /locus_tag="P5316_00535"
     CDS             complement(18454..21504)
                     /locus_tag="P5316_00535"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83466.1"
                     /translation="MTREALGKVVACTALAVSWLAAVPRASEGQAPDIFPFGHESDSH
                     VGLFAYYPDLVSWKNPDWWTNPDDAHMKPPIAQIFCDQLGERRGWIVFYVHEWKPTGG
                     KFPTNSSDPRWIELQSALAFAYQHLRLIPILRLHWLDNTGNSVEGWPPDWDMVPRGQH
                     WSVPVDTETYDSLRGANPEDPGYQDMALLMRTDYINWCTQLARSLRNECRWFIIGNEL
                     NHSAEYGGSPPYTEGGIVPPYWYASVWSGCFAGMARDENELGGPTKCQVLVAPLTPHS
                     PSTAGQPWQDKLRDYAKAVDVAYRGKFGDVPDPDSLLPGFAIHGYPDGYESSSGDCEE
                     HPIMNQFSQYISTIETTVHDDGSYPIAYAHDTEYIIDEYNSWTSADGDTTSYHEYSIR
                     GILDHVSELRSTWAPNRLRAACYYAYRVPMIYIDGNPQGTFYDLVEPDGLWPHPTDIT
                     LRWKKTGFMRRYLNFAFDELVEYTGCTPPSEWCGSQLLVFPDGTPVVGEFNNVLDGDP
                     ETYVAARLDTSPDPDYWKGPYLRFALCPCDLEASYVVESISVKIYVDSEVTNASVWLS
                     VGPNVLAGFQNVTLYDGCMFRTACPLTELYDQILVEVDCHESSGNTGLQNNIGVREIK
                     IVNAPPPPITVSDIDVGDVGQTSASVSWTTEVASTSRVDYGTTTDYGSYTYDSAEVTD
                     HAITLIGLSPGTTYYYKVTSTCDNYEDGIEFGQFTSMPAPPTIERSPATLSPSCTQGS
                     NAASQSFTVRNSGGGTLSYSITDNMNWLSVTPTSGTSTGETDTITVNYTTSSLTPGTY
                     NGAITITASGATNSPQTIPVTLTVTAPPPTITISNIYVDSITQTSARVCWTTNVASTS
                     RVDYGTTTSYGSYAYDGTQVTGHAITLTGLSAATTYHFRVSSTRSGYRGGVSEDRTFT
                     TAVGPIIITNIVATPQTFGAVISWTTNIPSTSLVEYRKAGAVGADGKWYKRGNEDLVT
                     GHSITLAGLSSNTTYDYRVTSSAEGYETATSTIRQFTTLYCGPN"
     gene            21956..23047
                     /locus_tag="P5316_00540"
     CDS             21956..23047
                     /locus_tag="P5316_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRR83467.1"
                     /translation="MQKRRDVPTLMDVARRAGVSPSTASFIVRQREPQFSQYASETVR
                     RVQLAAKELGYRPNVMAVSLGRRQSPFFGVSFQFISDVIADPMNVRPMLMWEIFRGIT
                     LAGQAHARYPVLLTSPDAESYMRDPGAIDHVIRSGLCGMIVSVDPALWGAYVSRWEDA
                     GIPCISIFDRGVAGGPRWFVDLDNRRVGSLAWEYLYGKGHRKVLCVWDHRKAAGVVDR
                     IRGFREAQRAGGYRSICLRLARRDPETQEDVQHDEALLIEAIRSTRATAIFATSGGVS
                     MDVLAAMSRRGLRVPEDCSVIGIDPLFTYAEFAHLAGRMTEIICPGHELGRAAANLLA
                     RRVDGEVSEPTGVLVQPEIIERESVAVIR"
     gene            23309..23797
                     /locus_tag="P5316_00545"
     CDS             23309..23797
                     /locus_tag="P5316_00545"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HRR83468.1"
                     /translation="MIELTDAQKADYFRRSYTAVDGLWFMKMEERYGFDPALDVDEEV
                     WRVMPKIQARKLKSLTGLESGMEALCECFTTKLTVEGFDFQASRDAGGGMTISMARCP
                     WYDLLAESNRRHLAGRVGHRICDTEYRVWATEFGPGIVVEFGERICDGCSRCVVRFKP
                     CS"
     gene            complement(23842..29475)
                     /locus_tag="P5316_00550"
     CDS             complement(23842..29475)
                     /locus_tag="P5316_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83469.1"
                     /translation="MTVESNNLTSWNPDERQAPLAPGRCASLAIVVTLLCAPAVFAQA
                     TALQITNIPDQVSGVPFQVIVTAVDGGGIPTPVTVNTTVSLTREAGTGALGGGGTRTI
                     TAGTTSTTYPAATWTTAGTGKQVRATATAGDLLAPAVSNLFTVTPGTPYKLVFSVQPA
                     SSTAGVTLTPTVAVQDVNNNVVTTDPSRNITLTISVNPSGTTLNGTVTVATSSGLAAW
                     TAVEAMNITKTGTGYRLLASHDGPALAGPSQTVQSNTFNITPAAHHHLAFSTEPVNAM
                     AGEDLLPAVRIRDIYDNDCTAAPNRSIQLSIITNPGGASLNGTVTKTTSSGVATWTAV
                     ESLDITVPAVGYQLRATNLTAPLLPGGDTVDSAAFDITHNVADHMEFTTQPVTTAAGA
                     AIVPVITIQDVYNNTCTTDDRNITLDFAANPGGATLNGTVTLPSVNGVATWTAAELMN
                     ITVAANGYQLRASHDGAAFATSDTVVSNAFNITGGAAHHLGFTIQPVSPTAAGANLLP
                     AVTIQDEYDNTVTGDDRTITLTFAANPGGAVLNGNVSKVTVGGVASWGAGDGLNITVA
                     ANGYQLRASHDGAAFAGSDTADSDAFSITATAAHHLAFSAEPVNSLAGESLLPAVTIQ
                     DTYNNTVTGDERTITLVLMNGGGATLNGTTSLLTTGGVAQWTAGQAMNVTKPGVNYYL
                     RASHSGAAFAGSDTADSADFDISHNVPHHLAFTVQPAHTIAGQNLVPAVTIQDAYDNT
                     CDTDNRTITLTISTNPGGAVLNGTAALLSVDGVATWTAVQALNITTAATGYTLRASGS
                     GVFSGSDNVDSNAFNITAYPVLSSFTVVPGANPVTVNNPITLTITARDVYSNPIPNYV
                     TLQTVNLTSTTGGDGTNIDWDNGPATLTDHLDGTADLGVGTTFDADGKILLDITNRRA
                     ESITVTANDAVTPAVGTSAAMQWNPWFPLLLHITPPGIPVAGVPFDVDIYVTDQFENL
                     TNVTQDCNIRLRLSAGTGVLGGNIDGTITTGNSGTTISGVTYDVAESNVVLVIDRLSG
                     NFLVAGTSDPITVTGATPVALRISDIANQTAGVPFSVTVKAIDGFGNESKVTADTDIS
                     LQLFLGSGLLEGTTTGTITAGTSSVTINGVELNTSQIGAVIRASRTGGDVLADGDSNA
                     FLVSPGAPSKLALDLIGNQAANVAFIVTAHVLDDYDNIVGVTTDTGVTVSLATGTGTL
                     GGTVSRTIPTGDSSIVFSVTYDTAEAGVSLRIDRTSGNALASGVSNTFTVNPSPPVRL
                     QFEPVGSATAGSAFSVTVAAVDLAGNEGSVASNTTVQITINSGSGSLSGTSTGVITAG
                     NGRVTISGLYYNYAETISLKASSIAGDVLAEGVSEQFSVSAGPAQCLRFSQQPTTSDI
                     GTPIHVAVEVTDGIGNRRFFLGDVTLSLVSPSGCGREIEGVTTVSGFSGVASFSVIIP
                     QPCDGYRFAASAPGLSGATSNPFTVIAGTDLSGNSISLLIGETETMLSFSYTVSGLSS
                     VEPFTISWGLRRDTTDGEAVDEVLGSIVASDTFYRTPGNHTIMLGDIRSQLNGRFQYG
                     DRVFVHLDSGNSIAETSENNNVDTTTPTVNLVNELLRLKPRGDEPTAEVTYVIDSPAD
                     VPSFAIRIGLDDDGDGDIDTTIAEGRPEDADLTPGVHAVSISLLSAFDAVPIASDGMV
                     LLTAQLNPEQEWAESIPVDDNLRSASMNYPVDLVLSGLEHVRAPRERIFNVRISYSLS
                     DMPVSEDFVIAFYASVDNSNAISEGDIRIERHTISNPADKTHGLHTKTFRVTVPATVL
                     PGADFVLKARLDDTDAVKEVNETNNAMAMPRYTPGSTRHLCGLIGSAPLTLAVFGLLG
                     MKLALPARLRRRSSHLKKQGISGAVGGGGDPPPGN"
     gene            30205..31989
                     /locus_tag="P5316_00555"
     CDS             30205..31989
                     /locus_tag="P5316_00555"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR83470.1"
                     /translation="MTTFVFLEVARAGPVPPSESIAATRPVSRMGDPWGMIYGGSGWW
                     EHNNPHRTNIAERAAAGAKEMAGWGVQASRVGICWHDVERERGTYDWTMPDAVIPAVA
                     KAGIHVVLCVATTPRWAWQHPGVEAILIERGQENLAGCLPHKPEFWPDYERYLTEVVK
                     RYGELVKHYEIWNEPDGLAGFRFMEPEPGHPIGFQHGGDPLWYAELVKRSYRIIKSLD
                     PQARVGIGSYEYKKAIEPFVLDASMAALLVEGRVSDAVRRAFGEHPDAPVSTSATISI
                     EVPDRRWILNDDRNGRRYRIVRENEGLKVTDRKTWFIERLYEEGVKDHCDAMSIHPYG
                     NPFGREWLGTVRRLMVEHGNADKPLWVNEYSLHGSGTSLAFNTVRQLRLLRETPWISM
                     AQPLVFASHLDKNISPSGRSLRAHKRMAEEYRPRREMVFDFEGPVLKLLDEWEWDATG
                     AGKDDVDEPELRQEFPHGGERCLTASTPEKRVRVWFSPYVKAQDRWFKAFVLAEPREK
                     DAQLSLSIGVESGDILQNLREVRPFMAAVPAEQWFEVSFPIDGSFPEWKDLTTVTIYV
                     EIDSDKPGLRVSVDDVRVGSGRTVASER"
     gene            32002..33786
                     /locus_tag="P5316_00560"
     CDS             32002..33786
                     /locus_tag="P5316_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83471.1"
                     /translation="MSKKNALIGRLLLAGSLVVVVERPCPAQATQPTTPAPVFVPAIE
                     GQKRVLAELGSILQEEKVMLSDGAVQLALETLLSDEQKQAEAIRELGRRTLGRTAEEL
                     SAEDRAERDRLFDGQDILTDRYRRLEKGMENKATIKPDSVYTGLLAKAADSQLLQTME
                     DAGRQVRANQLSNALRSTNKVIEVLREMVAMASQKAGSTQDMDNTNGDGGFMAPSLRY
                     KGAAEKLAEFNWIGASADPLGHILRGLRLLEELTERQRAVAAATKTQAASKQASADLA
                     KTETDIRRQALEVGAKLALLDPQIDQLIRKATASIDKARPAMEQGPLSDAVEPARRAA
                     DELAAATEYLRKLWQDILSRLKQHTQEAAAIEGAGGGIPHGMSKAQVEQIQKMVTMLL
                     RATAALARVADAQTAILDRTRLAASEPELPSLKPDQEALAGAINNDVVPFDVSASQLP
                     RDMLPQGVAASVNPVGDLLKDAVDRIGKAADALSKPDRAEAVKRESESLQAMTTAMDL
                     MIKALQKLLGQLAPSAITPSGPGAAGMALDPSGGGRQAGGWLFGLPSQQQESVRQAFR
                     ETFPRRYDRAIKLYYQSIAREQASDGNQ"
     gene            complement(34017..34391)
                     /locus_tag="P5316_00565"
     CDS             complement(34017..34391)
                     /locus_tag="P5316_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRR83472.1"
                     /translation="MQCPKCSSSMEKVTYGGVEVDRCTDCGGLWFDVGEREKLKAQRG
                     SERIDTGDPDRAEKLNKVGKINCPVCHTQMIRMVDLKQPHIWYESCTVCHGAFFDAGE
                     FKDYKERTIMDFFHSLMAPERE"
     gene            complement(34617..34940)
                     /locus_tag="P5316_00570"
     CDS             complement(34617..34940)
                     /locus_tag="P5316_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83473.1"
                     /translation="MCNIAPVILMIVLPMIGLFFWVRGLMRASIGDAVAGLAILLLIS
                     TGILIVLRDSGGFDIVGCILIVFGVGTVATRPFATEKPPFKRRDLIPAGILFVIFGIL
                     GLIAV"
     gene            complement(35129..36178)
                     /locus_tag="P5316_00575"
     CDS             complement(35129..36178)
                     /locus_tag="P5316_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRR83474.1"
                     /translation="MSNGRLMILFLTLTTLLPPRTVVAQAEKSVQSPEQPDMFVEMTP
                     GSEEAIRSALKFLAGCQLPSGAWMYGKVGPNVGVTSLAILAFLATGNVPEQGEYAPVL
                     DRGIGWVLANAQPSGFIHYADGSSHGPMYEHAAATLMLSEIYGMSERPEIAAAIQAAV
                     RVIVNAQNSEGGWRYQPHSRDADISVTVMQILALRGAQHAGMIVPAETIERAIKYVKL
                     CADRGGGFYYQTRSGKPGYARTGAGVTSLEVCGEFDCDETRRGLDYLIANLGADKERE
                     GNYHYGIYYAAQAVYQAKDPRRWQQWFPPIREQVLAQQREGAYWDSEHGPAYGTTMMV
                     LTLSIPCRYLPIYQR"
     gene            complement(36178..37974)
                     /locus_tag="P5316_00580"
     CDS             complement(36178..37974)
                     /locus_tag="P5316_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83475.1"
                     /translation="MIAAVLLDAWIRGPRWWRALLLVLLGTSVVWVLRRSIMPLWKSL
                     SFDSAALMIERAYPDLRGRLVTAVQLADASRRAGSGASDDLYGRVRTAAETTAPLLAT
                     KAVIPGESIVKRCAAAWLGVAIVLALAGFNRESASIGIRRVLLPWAQVDWPKRTHIRV
                     SSISERVVRGGTLIVAGRVTGAVPSSGTLRVRAGRSPADRAYFEIEENGDFRVQYRPV
                     MQDIEIWLEIGDNATSPSRVAMIPPPEIVAIRTECTYPEYTHLPPQQIEDGNVQVILG
                     TQLRLIVIASKSITDGRLAWEDGTSAQMTLTPAPGAETTLVVRSSQSYRVHLTDADGF
                     HNPDPVVYRIEMTDNQYPQFDRVTPSIDRRATPTAVLPISAEISDDYGAAMVTLLYRK
                     GTATDTGRIELPLNRSGKRAAVQYDWRLEPLDLKPGDSLTWWLEARDEGEHAARQEWP
                     VSRSRRLRIMSEVELARALNDELEQLMDKLSQLESLQAECAEAVNRIASGEQSGQSTA
                     GQERTRAEKWRQDRLARTAGQLAESIGRVADDYAASRIGQQERWLRLRSTAERLARLG
                     GTDMPAIVLALEDALNSFRGEQPPATRPAEAR"
     gene            complement(38135..40507)
                     /locus_tag="P5316_00585"
     CDS             complement(38135..40507)
                     /locus_tag="P5316_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83476.1"
                     /translation="MSGPHWLNWLLWPQQLDGQSSEVSLRAYPLLGTAGTIAAGIGLV
                     ILAIWISRTKGPDLSLRKRTALASLRIAAVACLVPMLLDLSLQVQVSHRLKPTLAVAV
                     DGSQSMSLQDRYATGGAGESAPASTPAVVSASAPAGVSRIDLVKSLLLADRDREGSRL
                     DRLSRSAELLLFTFDRTARPVGINGESAGLGELDASAPYTALTPSIDGIVERLRGRPT
                     AGVLLFSDGANNTGGSPLAACSRWKQAGVPIHTVTVGDANPRDIEVRQVLANDLLFSD
                     DPATVVVRLRQRGYQTRRVPVVLRSGSQELGRADAVFDENAGEINVAITFTPRQAGEH
                     TFRVEVAPQPDETAHQNNHRSFVARVTADKIRVLYLEGRPRWQYRFLRDAMNRDRRLR
                     LQILLTGAEPVANPPPPLIASLPNNRDAFKNYDVFIIGDVDPGILTAEQTEWIGAMVR
                     DEGAGMMLLAGPSFNPQAYAETSFADLFPVEAAEEAGRSPHRPRSGKDDLFVPELTPL
                     GQTHSAIQLGDDMESNLEQWKALPAVYWFAPVRKARPGALVLAVHPTETAQAGQNERM
                     PIMAVQNSGKGKSFFCGIDETWRWRFKQGDRVFYRLWGQVVQYLGAPHLGGESRQVHL
                     WTDRSVYNRNDTALVTVRAEELAGTELPVLVTESEDGRQQKVPLTPSPGAEHMYEARV
                     PLTVAGLHKLWLDGYALEASSIVEVQAPQLEPAEPAANPALMREIAENTGGQSGLASD
                     IDRVISGIDLSPRSVGEQRDYRLWDKPILVLMLVSLLAADWVLRRLWQLP"
     gene            complement(40535..42751)
                     /locus_tag="P5316_00590"
     CDS             complement(40535..42751)
                     /locus_tag="P5316_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRR83477.1"
                     /translation="MKLLNSELLWLLPLVAVPVLIHILIRQRLPRVRWAAMTFLLRAL
                     RKNRRRLLLETILLLIVRTAIVAAIIFVVLRPVARAGWQWLSGQRQRTVSIIVLDDSA
                     SMAASDGIRTRMDRAVVRIEEYLDQLPAGSDVAVILAADPPVDVIRQPTRDMNYVREI
                     LSRLAPRDSTAVIEQAMSSAADRLREEKTPNREIVLVTDGQAAGWQEDDAKKREAFAK
                     AGEVGEVFVLTVPPTPAGDAAVTDLTISGGPDALIPSLAATLWPTSINVRLTAFNTKE
                     TLETAIDLFVDGRKVTRRQVSVPPDQDLIVGFEHRFNSPGEHTVAARCDSDLYERNNQ
                     RSMLLKVRERINVLLVDGRPGPDPATSAGGFIRIALWPTDPQERESVPFFDVRTLSTG
                     SLESVQIGNFPLIILADVPALPSTAIGQIKSAVREGSGLLIIAGSQVNASNLAAMFFE
                     GGSGLLPVQLGPPIDLQPTDPPVGLALNEPLVPALSAFDDPILMQALSTVSFRTVRPV
                     AFAQGKNVQTWARFTRGGAALVANTFGRGRVVYFGGSADRQGGEFPLSPAFVPFFQQL
                     AFHLTFGQDEPQQPAGRALEWHPARTDGITLTYPDGTSVPAEAMVGEDPTTEQPRVLV
                     PSAQRGGIYTLTYNDATGTPRQAKKAVNLPADEFDPAMLALAQLREKPSLAKTRIIGP
                     DDSIRTALLTAKREAELSTTGIVILLILLAAELLLVKLFAPRQVDAQAMLKKAMGL"
     gene            complement(42748..43698)
                     /locus_tag="P5316_00595"
     CDS             complement(42748..43698)
                     /locus_tag="P5316_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRR83478.1"
                     /translation="MAGRREERLTDRKYVVFDVLDHIASLEMRARDLVEGTMAGLQRS
                     PYLGASSEFAQHRAYVPGDDTRHVDWKVYGRTERYYVRQFQEETNFVATFILDCSESM
                     RYGSGKASKLDHACLLTASLSYLALRQSDAVSVALFDEDLIAFLPPRTSLGSLGVVAS
                     SLETVRPERGTRLETVVPVLVEMIGRRGIVVIISDLLDVSSPGGEDDTAGILRVIQRL
                     RHQRHEVVVMHVLDPQELSFDFGGRIRFEGMESLPAAVAEPNKIRADYVDIIQKFVAE
                     TKMACAGTRSDYVLVNTAEPVDRLLVRYLTARGKIREGRR"
     gene            complement(43987..45000)
                     /locus_tag="P5316_00600"
     CDS             complement(43987..45000)
                     /locus_tag="P5316_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRR83479.1"
                     /translation="MSSTATFAGSDKELAVQLSSGYQTILSQLERVIVGQKQVIEELM
                     ITIFARGHCLLVGVPGLAKTLLVSTLADTLDLTFKRIQFTPDLMPTDITGTEVIQDDP
                     TTGERRFKFLRGPIFANIVLADEINRTPPKTQSALLEAMQERKISVGGVDYKLDDPFF
                     VLATQNPIEQEGTYPLPEAQLDRFMFLVRVGYPTDEEESEIVRRMTSPATFKAEPVLH
                     REGILAFQQLVRRVPAADAMIEYAKRLVRKTRVTEADAPDFVSKWVTWGAGPRASMNL
                     ILAAKARAILHGEAHVSWDDIRAVAKPVLRHRIILNFAAQAERISTDDIIEQLLAHVG
                     EKE"
     gene            45564..46499
                     /locus_tag="P5316_00605"
     CDS             45564..46499
                     /locus_tag="P5316_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83480.1"
                     /translation="MTAFTDVQVDLRLGPDGRIDVDIACVSCGYNLRGLAPDGRCPEC
                     GTAIGRSTRGDYLRFCDPGWVETLASGMNWIVAGLILAFVLGLVAGGAVGLTNRPAYA
                     MIGIVGSLVALIGYWRVTTPDPREPEPQGVDVRAIIRYGSVLNIVLAVASQIVTQMSL
                     YWKLLAFPSAVVGLIVTVSIFLYAKRMAVRIPDDNLASQTGTVMWGFVIMSVLGMLFL
                     AATMAFGPAFATMTVPAAGTGGATGPGTGGTVVAFPVGLILFASLGCVITVVSLVFGI
                     WALVLIIRYRRAFIQAARDARATWAVDPAGQQAMG"
     gene            46758..48233
                     /locus_tag="P5316_00610"
     CDS             46758..48233
                     /locus_tag="P5316_00610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR83481.1"
                     /translation="MGKTTVVARAGKCDLGKLVAPFAKLACVDRVLALVPPGIQTAGG
                     IEAIVTDQPDSGSALRSAIEKLTGDYVLFVDGDNEIRLGQYALERMMDVAEQTGAGIV
                     YADYVVDEEAGVADRPTIDYQFGSIRDDFAFGPLRLVSRKAIEDAAKRHGPPADVRWS
                     AQYDLRLKLSIDHALLRLPESLYTVVEDHSRNQHDEHFAYVDPRNVDVQLEREQVATG
                     HLKRIGAYLEPRFEPVPPPSERFEVEASVVIPVRNREKTIADAVKSALSQAASFPFNV
                     IVVDNHSTDRTTEILRDLAAGDSRVVHILPTRTDLGIGGCWNEAVASPRCGRYVVQLD
                     SDDLYNGHNALAIMVTELKKGPYAMVVGSYRIVDFDLNELPPGLIDHREWTRDNGRNN
                     VLRVNGFGAPRAFDASLLRRHPLPNVSYGEDYAVGLRFCRRYEVGRVWEPVYLCRRWS
                     GNTDAALPILKANQYNTYKDRLRTIEILARQEMNRREKTTL"
     gene            48370..49239
                     /locus_tag="P5316_00615"
     CDS             48370..49239
                     /locus_tag="P5316_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRR83482.1"
                     /translation="MNAMQVACVQFENTADIAENARRMIAILETESAAGTRLVVFPEC
                     SLSTYGSQIVRALTAEQINAALKTVQEACGRIGIYAVVGSAYIENGKRYNGAFVFGPD
                     GRLVKRYAKMHVVKPELFEEGDALAVFRVDDVPCTIMICHDERYPEIFRIPVLAGAKI
                     GIYISCESKTREKWDNYRCQVIGRAVENQISVIHCNSGDGGADEGSHGHSRIIAPSGN
                     VLAEASTEVGQVIRATIHPKESSSGHAERGAAKPSLHAFWQEGLRVLRRQNPEFFEAA
                     PAQPAADPSARSE"
     gene            complement(49310..>51241)
                     /locus_tag="P5316_00620"
     CDS             complement(49310..>51241)
                     /locus_tag="P5316_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HRR83483.1"
                     /translation="FEFVLIEDRVRSVSLAVADDAGTELLRGFYPMHSPQVSERARTI
                     AARFAEATRMLAKFRAEARAKAEPILAKARPLVEEATKPASEPEACSTEDWQRLASTV
                     GDLTMQLDGVTCHARMLAKMPEAEFAVGLESSMRKVMIKDHPFEGVFDDHYELALARN
                     EHEGFQVVVIPFGRDLADVTVSVGPLKSAMGQAFNGRIEVSLVGHVDVADNPPYDAEH
                     KGWWPDPLLSFLQKSDVKEGEHVAFWIDVATRVETQAGSYEGTLTITARDASPIGLRL
                     KVQVWDFALPNGTHLRNAFTYNEGPTGNFYKGRWNDEMRYRYYDFILDHRLNIDHLYR
                     QESPEIGLLKYGAGRGMNAFNVGGVFRQGKGDKDDPKLSQYVQELKNAGVFDRAYVYG
                     FDEAKREKFTEIREVFGEIRKRFPGLKTMTTAVDRSFGKRSGLREVVDIWVPLTDSYD
                     IEAARELRREGREMWWYICVVPTHPYANWFIEYPAIEARLLTGVMSYKYEVGGFLYYL
                     INLWEGNSRPISKGPYTDWNPGSLVNEEKKYTANGDGSLLCPGPDGPLSTIRLENIRD
                     GFEDYEYLWLLRETLERIGKRPPAPAHRAFLDRAAALLRVPDSVVTTTTNYTRDPQEV
                     IEFRARLAEAILEGQSLLR"
BASE COUNT         9389 a        15525 c        16158 g        10169 t
ORIGIN      
        1 ttggccggcg aggtgatcga ggtcagtgag ggctatgccc ggaactacct gattccgaac
       61 cacttgggcg ttgagccgac caaggccaac atgaaacgga tcgaagaaga caagcgcctg
      121 gccgaggagg aacgccgtcg gcgccgcgcg gcgctggagg aaatggccgg acgtatgacc
      181 aatgttgagg tgaccatctc agccgcctgc aacaaggagg gccatctcta cggttcggtg
      241 gggcctcgtg agattgccgc ggccttgcgc gatgagggcc acgacgtgga cgccaagcag
      301 gtccagttgc gggaaccgat tcgccagttg gacagcgtgg tcgtccccgt gcttctcgcc
      361 gacgacctcc aggttgacgt caaggtttgg gtcgttcgcg agacgggcag cattgtcgag
      421 gaggacgaag aagaggaacg tcgcaggcca gagaagccgg gcagggaggc gcacccatat
      481 gaccgcggaa ccggcgtcga cgctctcgac ctcaacctct gatgtccggc cggcgcccgg
      541 tttgcccgtt cgggtgccgc cccaggatat tgaggcggag agggggcttc tcggttcgat
      601 gttgctgaac cgcgaggcga tcggggatgt tctgccgatc atcgggcaga actccgaacg
      661 attctaccgt cccgaccacc gcaagctctt caacgtattg atcgagatgt acgaccgggg
      721 cgacccgatc gacctggtga ccgttcgcaa cgagcttgag cggcgggggt tgctcaagga
      781 ggtcggcgac gtcgactacc tcgtccagtt ggccgaatcg gtgccgtcgc acctgcacgc
      841 cgagcattac gcccgccttg tggtcgacaa ggccatgctg cgcgacttga tcggcgcggc
      901 ggggcaggtg atcgaggagg ctcacaacca ccgccaggag gcgaaggaga tcctcgatcg
      961 cgccgagcag ctcctctttc acgtgaccga ccagcgggtc agcgagcagg ccgagcctat
     1021 tcgtcgattg ctggaggaga ctttccacca gatcgaggcc cgcgaagggc actacatcac
     1081 cggccttccg accggcttca tcgagctgga tgacatgctc agcggcttgc agaagggtga
     1141 gatgatcatc gttgcggctc gcccaagcat gggcaagacg gccttcgggc tgaacgtcgc
     1201 cgaatacgtc gcaacccgcg agcgaaagcc ggtggtattc ttctcgatgg aaatgagcag
     1261 gcaggcggtc gcccagcgtc tgttgtgcgc gcgggcccgc gtcgattcgc ataggttgcg
     1321 gcgaggcatg ctttccagcg acgacctgca gcggctgacc gaagcctgcg acggtctggc
     1381 cgaggctccg ttgctgatcg acgacacccc gggcatgagt gtgcttgaac tgcgggccaa
     1441 gacccggcga ctggtcaagc gttttgccgt cgaggcggta ttcgtggact atctccaact
     1501 tatgcgggct ccaaaccgca ccgagagccg ccagcaggag gtttccgaga tcagccgcgg
     1561 cctgaaagcc ctcgcccgcg agctgaacat tccggtcgtg gtcatggccc agctcaaccg
     1621 caatcccgag cagcgcaccg gcaatcgccc gcgaatgagc gacctgcgcg agtccggggc
     1681 catcgagcag gacgccgatg tcattatgct tctgcaccgc gaggagtatt accagaaaga
     1741 aaaccccaac cctgagattc aggggctggc cgaggttatc gtcgacaaac agcgaaacgg
     1801 cccgaccggc atcgtcaagc tgttcttcaa ccagcgtctg acgcgtttcg acaacctcgc
     1861 cgcgggccag gagccgtcct acgtcgccac cgccggttac gattcctttg ccgagtatca
     1921 gtcggagact tcgcctccgg attacgacga gcacgtcgga cgttcattgc ccgcggccga
     1981 tgaagaatcc gcaccgttct gagttctgct gacgactgat ccgaaagctg gcggatggtt
     2041 cgccctggag aacgatctga tccggaatct caaattgcag atttcaaatc acagatgcgg
     2101 ctggtccgcg ggcgcggact ttacccaagg tggcgtgcag agcacgctgc ctacgcgata
     2161 cctctcaccc tatcccgcac cgccagcaga tccttcatca cattgtgcag cgattcacgg
     2221 tagccggtcg tgccgaaggc gtcgtggagc tggcgataca gcttgaagag ctggttgtag
     2281 acttcgtggc gtttggcgtc gggcttgtag agcttgggct tgaggccgca catcctggcc
     2341 tgggcggctt cgacggagtc gtatccgccg gtcgccgagc cggccaccac agccccgaag
     2401 atggccgcgc ccagagcaca ggtctgggcc gagcggctga tcttcatcgg ccgcccggtg
     2461 acgtcggcgt agatttgcat gaccagcggg ctcttttcgg cgatgccgcc gcagttgatg
     2521 acctcctcga ccttaacgcc gtactcttcg agccgctcca tgatcatgcg agcgcccatc
     2581 gcgctgccct cgatgagagc gcggtaaact tcggcccgcg tcgtgtgaag cgtctggccg
     2641 acgagcaggc ccgtcagccg cgggtcgacc agaatggtgc gattgccgtt gttccagtcc
     2701 agggccagca acccgctttc gccgggcttg agcttggacg cctcctgggc gagcctggcg
     2761 tgtgcctttt cgccggcgtc gaagtagttg acgaaccagt tgaagatgtc tccgacggcc
     2821 gactggccgg cctccaggcc gaagtagccg gggacgatgg acccgtccac gatcccgcac
     2881 aggccgggga tgtcgggcag agggctggtg ttcgggtgga tcatcatgtc gcaggaactg
     2941 gtgccgagaa tcttggccag cgagccgggc ttgacgcccg cgcccaccgc gcccagatga
     3001 gcgtcaatcg acccgacggc caccggaatg cccgcgggca agcccatctt ggccgcccac
     3061 tccgggcaca gcatgccggc ccgctcgttc gaggagtagg ctttctcata tgttccgcgc
     3121 cgccgcagat cgccgagagc cggatcaatc cgatcgagga agtccgcggc cggcaggccc
     3181 caactcgcgt gatacatcgc cttgtgacct gcggcacaga tgttgtgttt ccattcggcg
     3241 gggtttttgt tgccggtgat cacggccggg atgaagtccg actgctcgac ccacgccgcc
     3301 gtcgcttgaa tgacctcagg tgcgacgcgg atgcagtgca acgctttcga ccagaaccac
     3361 tcgctgctgt atgtgccgcc gcacttggcg aggtattcag ggtgttcctt cgaggccgcg
     3421 gcggtgatct gggcggcctc ggcaaacgac gtgtgatctt tccacagcca tgccagggca
     3481 ttgaggttct tggcgaatct tctttgccgg accagcggct cgccggtcgc gtccaccggc
     3541 aggggtgtgc tggcagtcgc gtccacgccg atccctatga ttccctcggg cttgaagcct
     3601 ttgaccgcct tggccttcct gagggcgtcc ttcgtacact tgcacatcgc cgtcagccag
     3661 tcatccgggt gctgtcgggc gacgttcgga tcacgccggt cgaggatgat gccggcttcg
     3721 ccgctcgaat accccgccac ggccgtggcg acctccttcc cgttggccgg atcgacaatc
     3781 aacgcccgag ccgaattggt cccgaaatcc aaaccgagtg cgaatgcttt gaccttggcc
     3841 atggtattcc tcgctaggtg ctcgcagacc gcagtatgat cagcggccga tggaattgaa
     3901 gcggcggcag cgaaaagtgt caagagaggc acgccggcgg ttcagacaag gacacgtgga
     3961 aggcgacaaa cccctgccga cgatttcagc aacccacgcc ccctttgccg tggccgtccg
     4021 gtaccgaccg accccggcaa agagggacgt gtttcaacgg cagcagccgg tcaggcggca
     4081 ttcaacggct tgcccaaccc ccgcgaatcc ctgtccgacg gctcaaggca cacacccttc
     4141 gggcgggttg tgcacgtcca gcagtatgcc gggtcccgtc gagcaacgtt cgaacgccat
     4201 gtagtccgcc agatccacgt cgagatcgcg atcgtgatcg aagcacctgc atgagtaagg
     4261 cgcatcgggc aacgtcgtca caccctcccc ggcgaagcac acctgaaagg ccgcgaagtc
     4321 cgcctgatcc acatcgctgt cgttgtctgc gtcgacttcg accggcgagc agcctccaac
     4381 cagagcaatg cccacgttgt cgacgtggct cacggcacgg ccgacggatc ctatcgtctc
     4441 gaccattacg gtggccaatc cgctgaagac cgtgccgctc acctggacct gctgccaggt
     4501 tgccatggga gataccacct caacggaggc caactcagtg ccgttctcgt caccgtcaag
     4561 cagaatcaga cgggtggggt gagacatgga ggtatccgct attttattgt acaggtatgc
     4621 ggaggcgtca acccgttgac ctatcgaagc cttgatggtc tggaaagccc gcaagacgac
     4681 cgtgtcgtat gcggtgaaac tcgcatagtg atcaccttcc tgggggcagg gggccgagaa
     4741 gtagacgcga gtatcccgca tccagctgat ttcggccggg accgtgtccc agtgcaaggc
     4801 ccgcgagcgg tcataggtcc cgttgacgca gggaccgccg ggcgggtcgt tgggattagt
     4861 gccgatctcg aaactgagat tggagggcgc ggcagccacc accaggaatg ctcgatacag
     4921 cggcgtggca ccgccaacca ctatgggcac tacgttgtaa cgacccattt ccttgtcgtt
     4981 caagtcgaac gtggccgtcc gctcggtctg gttggagccg gcggggccga gagtgccgat
     5041 gatctcgctg ccgccggtgt cgcgtaccag cttgatgccg ttgagaccct ccaggttcgt
     5101 gccggtaagg gtcaccaccg cgggtccgtc gtttgtcgca tgggcagggc tgatgctgtt
     5161 gagttggacc gcatacggtt ggacgacctg gaacgcgttc tgcaatgaag tgtcgttgac
     5221 gcaaccctgt tgcataacca cgacgttgta ggtgcccacg ggggcattgg tcagatcaag
     5281 ctgacagcgg atgctggttc ccccgacgac ctgcacgttt gtggccatga ttgccgccgg
     5341 cccgaccagg atcacaaccg gaggttccgg tgaagatacc aggccgctcc cgggaatggc
     5401 ggtgatctgc accaccttgc cggattcgcc ttgcgacggg gtgatggtgc cgttgagggt
     5461 gaagctgtcc acgcacggtt tgaccgagag cgcgtcggca tggagagaga tttcggtgct
     5521 ggtgacgtcc ttctgttcac agaagaagaa agcggtcatt cggggtgtgt tcgcgaccgc
     5581 gctatagtaa gcgaacttcc agttggacaa gctggtgccc gaggtgacgt tcaccaggat
     5641 cttgttattg gtcggcgagg tggtcaggtt tgaaatgcgc accgagctgt cgccgtccag
     5701 aagccccatt cgggcaatac ccagggccgt tttccaggcg aaaagcccgc tgaaagtgta
     5761 cgtttgaccc ggcgtgacac tgaaggtttg aaacgccgtc gcactgcggc tcgtatcgat
     5821 ccctgccgtc aaccgggcca tcgtaccgta gttattgccg tccgccgggc aggtgggcgt
     5881 ccaggacaag gggttgttcc cgatggtata gccgttgagg ttgaggaagt tctgccgcat
     5941 gtcgggattg gggacataaa gatcgcccca gatggtgtcg tgccgccagt gaccggaatc
     6001 acaggccagc cagtccgtcg ggaaatccgg cgtccgcgtg ggggtgcaag caggtgccgc
     6061 cgggctctcg aaactgccgt tgctgaaagc cggcaggtag acctccagcg cgcccggggc
     6121 gaccgccgga acgcagcccg gaccggtcac cacgatatcc caggggccca aggccatgtc
     6181 ggcaggcagg acaaaatcgg cgacgatact ggtcgccgtc gaactggtga tcgtgccgac
     6241 gatatctgcc gagcccttcc gcttgagttt gacggccatc tgatcgggcg tgaagttcaa
     6301 gcccgtgata gtcagagtct gcgtactggc tcgggggaca ctgcgatcgc cggtctgggt
     6361 gctgatgacg ccgacggcgg cgccggcgtc gtttgtgggg ctgtctatct ccggctgaag
     6421 cgtgcagtcg gtctcctcgg ccaggacaag gtcgtccacc acgatcgctt ccatgtgaga
     6481 ccatgtcgcg ggggccaact gctcggccca gatcttcacc gtgagctcgg cggttgtggg
     6541 ggtggcgatc aacggcgtga actcttccca gccgaagaag gggaaactat agcggtcgcc
     6601 gacgatgacg gagcggtcct tcagcaccgt tcccgtcacg gggccgtcga tgagttgaac
     6661 gtgggcgcgc attttcgtcc accgaccatc ggcccgcatt ctggacatcg cggcccacac
     6721 acccgaaagc cgatatgtct tgccgggttc cacggcaacc gtctggtata agtacatggc
     6781 agcggtcagc ttgtcggcgt tctcgagggc tccgtaataa ccgtccgcgt tcggtcctcc
     6841 agggcagagg ttgataagtt cccagtcgtt ttcctcgttc ttggcaaacc agcggctgaa
     6901 gcggttccag tcgctgcatt gccagcctgt cgggtgtgtc ttgtctcgcc agccgcgctc
     6961 ggggccgcag gccccgggtc cgaccgggtc tgaaaagccg ggattgacca gcagatttgt
     7021 ctgtccgaac gcgggcaagc acagcgccag ccccgccacg gccaggatcg tattgcgcat
     7081 gggcacatcc tccactgtct tctgtttttt ctgaaatctg ttctctgcga agctaccggc
     7141 ccgagctatg ctcgggcacg ggcctgattg ccggcttaat cgctgcggac tcttgccgca
     7201 cagatttgct cctgaacgca gcgaaccgac cggatcacag gctccgaccc gtggcgatcg
     7261 gtgggctgca gtgatcactt gtaactgcag cccacctgtc gctcatcatc gggatttgct
     7321 cacaaccatc cgggttgtac cggacaggct cggctacgac cgccttctca ggaacatcgt
     7381 gcccgcaagc agcagcagca gcgatgacgg ttcgggcaca gatgccagga agtccgcgtc
     7441 cgtcggcacg tagtccacga agcccggttt gtctggatgg ccggttctgg caaagcagat
     7501 cacgtcgatc cgcccgttat agacaccggc ttcccgctcg tacagccgga aggtgatctt
     7561 atcgtcgacc agattgaagg tcttggccag gaaatcccga tcgtcgggtt gctcagtgtt
     7621 cagccaggtc cagttgggcc ggacgccgcc gatgtagtcg ggatccaccc aaatgaagtt
     7681 ctggagaacg cggtcgtcat cattggctct gccggccagg gccgcctgcc aatcggcctc
     7741 tgtggggttg ctgacattca ggtcggaggg atgaccgttt acaaacaacc agtcggaatc
     7801 ccagaacatg tcaggatttc cacccggcgt cccaccgtac tcagccgcat cagcaatctg
     7861 gctgcggaac cagacgcccc aatcccccgt cagattaaag tgggccggca acgcactctt
     7921 cggaatctcg tattgaaccc accagtcctc ctgatagccg ccgctgtgcg accaactgcc
     7981 ggcgtgattg acatccttgc cgaagtagta ggcactgcca ctgagcgggg cattggcctt
     8041 cggatccggg aagtagtcgg gtgtcccgtc gctcatgctg cgggcggtga aggcatccgc
     8101 ggtgatccat atctgttgga taccgccttg ctccgagtag cggacacccc cggctgctgg
     8161 tgagaccagc aagagcatcc ccacaaggat ggcagtgcca acctgtattc gtgtaccgga
     8221 catttgcgtg ctcctctctc tctaaaacag aaactccaca tccccggcgc cgcacgcgcg
     8281 gtgccgttca cacccactgc aaggttttga tagttgggat ctcgtcctca actctcccca
     8341 tttgacgtct ccgtccgaac tgaccggata ccgatgtgag ccgccttttt cggccgccga
     8401 agccgccaaa ggcgaaacag ataataatgc ggcggcgggc cgagtgtcaa gaaaaaaaga
     8461 gggcgttcag cagattgatc cctatgccat agttgggggt ttggcggcaa gattgcaggc
     8521 cggacgctga agacgatcgc aaagactgct cctcgaacgg tttaaagtgc ttgtcgaacg
     8581 ttgggtcagg tgtcgggttc tctcagcagc gaccgtatga aacgcaagtt gacgcgatcg
     8641 aaatcccgac cgcgagtgtc ggctcctttt ggcgtttcca agatcatcgg acggcccaag
     8701 aaacgcgggt cgttgaccag gcggcggaaa gcactgcggc ccaggcggcc tttgtcgatg
     8761 tgttcgtggc ggtcaacgcg cgagcccagc ggcttcttcg agtcgttcat gtggaaacac
     8821 agcaagcggt caaggccgat gatgcggtcg aatgcctgga aggtttccgc ataacccgca
     8881 tcggttgtca ggtcataccc cgcggcgaag aggtggcagg tatcgacgca aacagcgata
     8941 cggtgggggg catcagtctg cgaaatgatc ccggccaggt gctcgaatcg ccagccgatg
     9001 ccggtgccct gaccggcggt ggtttccaga acggttcttg tgcgcgagcc ttccgttcga
     9061 gcgtgaatga cgttcagtgc agccgcgatc cggcgaatac cccagccctc tcctcggccg
     9121 ttgtgtgagc ccggatgcac caccagcccg acaatcccca gccgttcgca acggcgcagc
     9181 tcatcggtgt acgagtggat gctgcgccgc cacaaggcgc ggtcgggcga ggccaagtta
     9241 atcaggtaag tcgcatgggc cacgaccggg ccgagccccg tcctgcttcg ggccgcctcc
     9301 cacaagcgga ccgcctcgac cgtcaggacc ggagcgctcc attgccgctg attcttgacg
     9361 aatacctgga aacagtcgca tccgacctcg accgcccggc gaaaagccgc gtgtacgccg
     9421 ccggcgatgg acatgtgagc tccgaagcga cgctggccgg ccggtacgtt ggattctctc
     9481 ttcgccattc ggctcacgtc cttcgttcgg cgtggattgt ggtgccgacg tccccgtcgg
     9541 cacatgctcc cgccctgtct atcgctaatc gccgagagtt gatcgctttt cttcgcagat
     9601 tgtaggatgc accggcagaa tgacaactga gcaccggctc acgaacaagg ttctttgcaa
     9661 ttgtgtccga cagcctgcgg aaataatatg gaggtttcgt gctggcaggt ggcgggggtg
     9721 tccgtaggga ggcggtcggc ggttcagcag gaacggagga gccgagacgc cgtgggtagc
     9781 ctctgagggt cgcaaggggc cgtaaaagag cgccacggcc cgatcgcccg gccggtgtgt
     9841 gacgcggatc ttcttcggcc tccttcggca aggagaatct ctcgtgaaca atccgtggca
     9901 atatgatgag acgattcaag tcggcacgga ctatcgcgac gagaacgagg tgcgggtcta
     9961 cgaccgacgc atgcagacgc tgcgcgacgt gggcggtgag gtgaaggaca tccagagggc
    10021 cctgctgctc tcgtcggatt cgatcgtgtg ggagatcggg accggcacag gggaatgcgc
    10081 cctcgctctt gccccggcgg tcaggcatgt gtatgccagt gatgtctccc cagccatgct
    10141 ggaatatgct cgccggaaag cggagcaacg cggcgtcagc agcgtgacgt tcgaaaaggg
    10201 cgggtttctt tccggcttcc ggccggatca tccagtggac ggaatcatca cccagttggc
    10261 gctgcaccac cttccggatt tctggaagtc gcgagcgcta gatgtgataa cgggaagcct
    10321 tcgtccggga cggcgactct acctgcgcga cgttgtcttt ccttcaacga ccgatgatta
    10381 cgatgtcttc ttcaagacgg tggtggatgg agtccggtca caagcaggtg acgagatggc
    10441 ccaacggacg atacaacaca tcaagacgga gttctcgacc ctggattgga tactggaagg
    10501 aatgatgatt cgcagcgggt tggaaatcgt ggagaaaagc agcacgggtt tcgtgtcggt
    10561 atatgtgtgc gagaggatca cggacgggga aacggctaaa tcggggaaca ccgtaccgtt
    10621 tagacccacg aggttttcgt gacgcgcccc ggaaacgaga ccttcgctcc ccaaggcggc
    10681 tttccgccgc aaccaaaaga tgcgcgcaaa acgcacacct ggtcgcgacg caagagactc
    10741 acaaaccggc aatgcgggtg tttccttttc tgcaaagcaa caggggagga cggaatcgga
    10801 ttctcttgag ataaccggca ggacgtctct gggggagacg ctaaataacg tgggcggcga
    10861 aaaacgagca cgagccgacg cgcggtcttg tgctcgtgct tgcctttcgc aaacacacta
    10921 ccctttccct acgctcgtgc accatcggta gtagtatgtg acagacacgc ccgggaagac
    10981 aatgacttcc gcctttccgg tccggaatca cgcacgtcat tcactcaaac aagctctgtt
    11041 gaccggggac gtcgttgcgg ctgcgatcgt agacgctctt ggcctcgtcg ataagttcgc
    11101 cgagatcggc aaacctgcgg tagacgctgg cgaagcgaac ataggcgatg gggtcgacgt
    11161 cgcgcagctt cgcggcgacg atggcaccga tggtctgact gctgacttcc cggtcatgct
    11221 ccttgaagac cgcatcctcc actgcgtcaa cgagtttggc tagggagtcg tcatcgaccg
    11281 gccgcttgta acacgcccgt tgcaggcccg cgaggatttt cgaccgatcg taaggaaccc
    11341 gggtgccgtc gcgcttgata accagaagcc ggacctcgtt ttcaacccgc tccttggtgg
    11401 tgaatcgttt gtggcaaaac tggcagactc gccgacgacg aatgacctcg ccgccctcgc
    11461 tgagacgcga gtcgatgacc ttgtcctgat tgatttcctt gcagtacggg cagcgcatgc
    11521 ttatatgccc aaagtcacca agggggcctg ttgcgtccgc gaaccgatcc ggccccgggc
    11581 cgccagtgcg ttccagacac tcggccgatg tcatccaggg caggcaccag atctggagta
    11641 taacgacatt tggcggccca agatatagcc gccgatgcaa aaaacgcaag cgccagtgac
    11701 aatcggtcgg ctcgtgagtg cgagataatc tcggcggacg tgctactggc atctcgtctg
    11761 cccgtctcct ccgcatggtc cgaccagcgg cttgttgtac tcccagaatg cccccggcga
    11821 gttcctcgca cactccaaag actatctgct gcttagggcc cccttggcaa aaacggcgtt
    11881 tgtgggcata gtgggcgaat ggcttcgaga ctgattgaga agaccgttgg cggcgttcgt
    11941 ctccagggct tcagcctggc gggggaggag acgatcttga ttgcccccga gatgaacgtc
    12001 gcttttgaca tcggccgggc cccccgtgag gcaatcggaa ttgaccatat ctgcttgtcc
    12061 cacgggcaca tggatcacgc ggcaggactg gcctactact tttctcagcg ccatttccag
    12121 ggtaacgctc ccgggacggt cttggtccat cacaagctgg tcccggccat tgaggatctt
    12181 cttggggtct gggggcgtat cgaggggcac gtttcccctg gcataatcgt cggcgttgat
    12241 gaggacgagg actttcaatt gcaccggtcc ctggtcatcc gccctttcag ggtctgtcat
    12301 ccggggccga cgctgggttt ctcgctcgtc gaggttcgta agaaactcaa gcccgagttc
    12361 gccgcccttt ccggccggca gatcgccgag ttgaagcggt cggggcgaat tgtggaagac
    12421 cggcttgaaa tcccgaggct ggcgttctgc tccgattcgg ccatcggctc atttctggac
    12481 cgggatcatg ttcgcaacgc cgaggtgctg atcttcgagt gcacgttttt cgagcccgag
    12541 catctcgagc gcgctcggca gggccggcac acgcatatcg acgacctgcc tcagctcttc
    12601 gagcgggttc gcagcccgca cgtggtgctg atgcacatga gccgtcgaac gggcatcggt
    12661 gcggccaagc gatatctccg caagttgctc aagcccgtcg atctggaacg gcttacgctg
    12721 ttcatggatc tcccgccgca acggcggagc cgtcaggctg atgaggaggc ttgaccctca
    12781 gtgatagcgg tcctcgcgga agtggttggg tcttcggacc tgatccgttc ggcgggatga
    12841 ccgtgggcgg caggaggggc agcgtttgtg gctggggagg tcagcccgag cggaaataca
    12901 acggctcgcc atttctccgg gagcatggcg agccgccggc tgggcattgg ggttgacgat
    12961 catacttcga cgaccgaatt cagctcgccg aaggggttgg attcgacata ttcgttgttc
    13021 gggtcgcttc tccactggcc gtcgatcaca taccgatagc gatagcgacc cggcggcaat
    13081 ggcaggagcg ccacgaagct gttcccgtcg ccgttgcgct ccatgagcgt cgctccgggc
    13141 tgccagttgt tgaagtcacc ggcgatggcc acactggtgg ccccaggggc ctcggtcgca
    13201 aacaaaacgc cctcgttagt cttgtggact ccgtaaatgc cctcgatctt cttctgcact
    13261 tcctcagggg tggcgggcgt ggcatcgccg gcttggctga gctcgccctt tccgaagagc
    13321 ggcttgctgg ttgccaagag cttctctgcg cgctccgcca gatcgtcggc ctgtcgcaga
    13381 atcgccgttg gcaccatctc ggggccgccg gacgagatca gctcgcgggc caggcgggca
    13441 aaatcgcgat aacccatgct gttggggtcg tattccgtga tgggctgtcc gaaactggct
    13501 ccttcacgaa gcttggtgtt gaagttgatg aacgtgtggc acatcaggtg tccgtacttg
    13561 cgtttcagct cgttgaggat ttcgcggccc agtttggtgc gaacatcgta aaggttggcc
    13621 aggacgcgaa tctcaagcgg gctctggcgt gtgctgcgaa tggcctcaag ggtttcgagc
    13681 tgtttggtca gcccctgaag cgagaaatac ccggtgtcca cgggtatgat cacttcgctc
    13741 gccgcgtgaa aagcgttgag tgtcagcaga ccgatatgtg gcgggcagtc caccagagcg
    13801 aaatcatact tgtcactgac cgaaaccagg gcgtcgccca gtctctgttc cgcgtccggc
    13861 agcgttggtc cgatgagttc aaactccgag agcgcggtgg tcgagggggc taggtcgaag
    13921 ttggccgtaa tctgccagat acagcgggtg atctcgatgc gatctccgtt gttgcgatgc
    13981 atcatgacat cgtggatgct cacctcaatc tggtcttcgg gaacggccaa tcccaaagcg
    14041 caatgactct gggggtccat gtcgaccagt agcgtccggc gaccctcccg agccaggcaa
    14101 gcggccagat tgatactaac tgtggttttc ccacaaccgc ccttctggtt ggcgatggcg
    14161 atggttctca tggtcgttgc agccttccaa tgagtacaga tgaaactctc caggcatccg
    14221 ccttgacccg gaccgcagtt ccacagcgcg gctcgctgag ccgacacctg cgcttttatc
    14281 ggtcgcatgt tgcgcaaaac tttaccgccg cgccgatcga ccagaagttt tttcggacca
    14341 aaaggttagg ttaagatagg caagatcaga atacggaggt tgcggcagct ttcagggttt
    14401 caagacgtgc ttctggcatg tgatctgcaa ggcatcgtcg cacagccatg gcggcaaggt
    14461 cgcaccggca cgccgcgtct tcgagcaagc ggcgcaccgc ttggatgagc tcgcgacctg
    14521 tcaggaaagt cagcacctcg tggtcgggcg cgagcagcgt ctccaggccc cccggcaagg
    14581 tgttcgaggg atgtcggcgg gcgatcacgg cggctccaca cccggccgca agcagcgcat
    14641 caccggtcag caggccggtc acatcggcgt gaataacaac cttggcgcag cgaagcacat
    14701 caaccttttg gcgaatcgtc gtcaaaggcg ggctggcatg ctccggaaaa gcggctgtcc
    14761 agcctggtcc atggatacgg aatgagagcc cgcttgcctt cagttggccg gcgaggaagc
    14821 ggcacaggag cgagggagca agctggcagg tcaggtttcg caggatttcc tcgcgcacct
    14881 gcggctcgtc gatacgggca cgtgctttcg cctcggttcg actcaggatc cgctccgctt
    14941 ggtcgtccgt gaaggtttcg atttgggtcg ccagtagatc cgcagcgcct ttccagagct
    15001 gggcatagcc gggcagacgc ggcgcaatcg cggcagcgtc aatcgggcct gcgtcggtga
    15061 tcagaagcac gtccagtggt cgttcctttt caaggcagtc cagctcgacg gggcccaggc
    15121 aaggcaacgg acagacggat aagcgtcttt ggtcgatgcc cgcagccagc gctctcttga
    15181 gaaggaccgg acttgtgacc gccaccgcac ccagcctggc acgatggaac acgtcccccc
    15241 gatggctgag ccagcagacc acgggtatcg cggcgggtat gacatcgcag acctgctcgc
    15301 ggatgacatt gagcaggatg gagaagctcg gcgggccatg aggcgattcg agaacgcgtc
    15361 gcgcccgggc gagcgaatgc atgtcgccgg gcgttcggat atctgccacc gtggcatgcc
    15421 agcccagctt tgcggccgct acggacaggg cgtcggcggt cgaccacgtc tcatcgagcg
    15481 cgtggagggc tagaatcagc acgtgtgccg gagcgtcccc ggccgcaggg ctttgcgggt
    15541 gtggcattgt acgccagcga tggtgcacct gggccagagc ggtctggcgg tcccgctcgg
    15601 ttcgttggta cgcctgctcg acggcaaacc gaatcggggc gatctcggcc ggggtctgcc
    15661 agggaaacat catcatctgg ttggggcaaa ggtgccccgg gttcctcatc agccactggt
    15721 acagggcgtc agccagttgt cccaggcccg atgtcatcag gacaagtcgc ttcgctgaga
    15781 tggcggcggc caggtcgtgc agccgcaggg ccagcttcag gtttaccgga tcgggttccc
    15841 agacaaaaat cgcgcgatga gcgcctaggc gaggcagcag catttcgacc tcgctgccct
    15901 cgcccatgcc agggaaaaag acgttggccc tgcccgcatc gaatcggtcg agcagggcgg
    15961 cggcccgcac gccggggatc gaggtccggc cgaaccatgt catccatccg tcagattgct
    16021 tcaaccggaa gctgagtctg ccgtcgcgag tgcgggcggg catcggcggg ggaccggaag
    16081 cgtcaggcag atcggccaat cgctgggcga gttccggatc gacctcacgc agggcggcca
    16141 ggttgcgctc gaggatgagt gggtcgaggt tgggtgtatc gtccttgacc atcgacagcc
    16201 gcctgcgagc cttccgggct acgcgtggta tgtcatccgc aggatcgtca tgaccaacag
    16261 gaagatcaac cagcagccgt aggccactgc ggcggcaatc aacggcccac gcggcctggg
    16321 cggagagtcg cccgcgggca gatcgttgtc ccaaatggat ttgggctcac gctgttgcga
    16381 gtctttcttg gtcgtatcgg gcatggtcac tcaacctgtc tatcaccggt cggcccgcag
    16441 gactctcgcg tttgcctcgc acatcgtccg cgtcaaagtc gtgccgccgc ccgtcgacgg
    16501 gccaagttac cagcatccgc gacgcgtggc aagaatggtt gggggactgg tcgggtcctg
    16561 tgtttgggca ctctgcgagg ttccgatcgc gagcagcagc ctttgggggg cgatctgcaa
    16621 gccgccgatc accgggcagg cagcgatgcc cttgcttgac aaacggccgt gatagcagga
    16681 attgcagggt ttgcggaccg agattatggc tgaataacgt ggctgacaga caaacctgtt
    16741 ctgctttcct cttgcgaact cacggcggat cgggcgtgca gtccgtgggt ccctccgtcg
    16801 gaagcagtcc aagcaagttc tcgttattca gcagggcggc atccagcgga cgttcgagga
    16861 atgcacggac gtcttcctga atccgtttca tgtcaataga acgaactcga tcgcgaacca
    16921 gcgtcgccca cgctttcgca tcgtaacggc caactccgtg cgtctggtca agggcatttt
    16981 gcaggagcgg cagattcggt tccacgggcg gttgccgcga caggtaccac atgagatcgt
    17041 accaatcacg tcccttggcg tatctccggg taatggctgc gtggagtttg cccgacagca
    17101 aggatggcag atcgtaatac tgaaccagga aagtcaggtg tcggctgatc acccggcgtt
    17161 cgcagcgtgc cccggccggt ggccgggtgt cgatctccag tttgatggcc agcttctgat
    17221 cgggcatcgc cgccagcccc gcgtcatgga ggattccagg aagtcgcacc cacgccgtgt
    17281 gcaccgcctt gcggtcgttc cacgtcacct cggcgttgag cccgccgagg gtcagatctc
    17341 gcttcacctt ggacatccac tcccggccgg cgtagccatc agcggtgacc agcgagaaat
    17401 cgagatcctc cgagaaccgc ggaagaccgt gcaggaagcg aagcgcggtc cctccgacga
    17461 aagccagcgg gcggaacgcc tcggattcat gaaacgaccg caggatgagc gtctggagat
    17521 actcccgcag ttgattgagg gcctgatcgg gatcggtcac ctctcgaacg attgccaacg
    17581 cctgctctct cacagcgtca ccgtgccctt tgcctcttca gcggccaggg taagccaccg
    17641 ctcgaccgcc cggtcaagcc gggggcttcg aaaacacgtc gcgtattccc tgagtcgatc
    17701 ctctgccacc cggtcaacgt gctggtatcg catctccgca agtcgttcgc tggtccattc
    17761 gcctggggtg aggtgccaat gatccagcag cgccttctcg ggttttgcga ccaggatttc
    17821 ctctccgcca tgccttagcg acgaaaaacc gaggaagcaa tcctgtttga tgttccgata
    17881 atcgaaaaca cccaacgggt tctcgaagcg acggggaaca cgcggagtca cgctagtcaa
    17941 ccagaccacc cgttccggaa tcatgtcata gaagccaaga gcccaaagcc cgctgaggta
    18001 ggacggccgg tacaggtcgt ttgccagcct ggcgggtgtt accggcgcgc gccggtacgt
    18061 gtcgggcagg gtgtagacgc cgcgacgcag tccgataagc ttgccctgtt tcatccagcg
    18121 ggagagctgt acgcgaatcg cctcccgccg gtcatcgaag gactggacca gcagtgccag
    18181 gtcgaagcac ggcaacgtcc cggtcagctt caccagcgag ccataatcca tatacgaaga
    18241 atagcataaa tgatatctat tgtcaataga aatggcggca agcagattag attatcggcg
    18301 ggtgacgccc tgggccgttt cttcagaaag caccggccgt ctagctccca caagttctga
    18361 cgctgaggcg atctgctctc tgtcggccac gaccagcagg ctcaaagagg gcgagacatg
    18421 cgcgcagggg cgcgcatgtc tcgccgcggg tatctagtta ggaccacagt agagcgtggt
    18481 gaactgtcgt atcgtcgagg ttgcggtctc gtatccttct gccgacgacg tcacgcggta
    18541 gtcgtaggtt gtgttgctgg atagccccgc cagcgtgatg ctgtggccgg tgaccagatc
    18601 ctcatttcca cgtttgtacc actttccatc tgcacctaca gcaccggcct tacggtattc
    18661 gacgagcgag gtgctcggga tgttcgtcgt ccaagagatt acggcgccga aggtttgcgg
    18721 ggttgccacg atgttggtga taatgatggg gcccacggcg gtggtgaacg tgcgatcctc
    18781 cgaaacgccg ccccggtaac cgctgcgcgt tgagctgacc ctgaagtggt acgtggtcgc
    18841 ggccgacaag ccggtcagcg ttatcgcgtg gccggttacc tgcgtgccat cgtaagcgta
    18901 ggagccgtag ctcgtggtcg tgccgtagtc cacgcgcgag gtactggcca cattcgtggt
    18961 ccagcagacc ctggcagacg tctgcgtgat gctgtcgacg tagatgttgc tgatggtgat
    19021 cgtcggcggc ggggcggtga cggtcagtgt taccgggatg gtctgtgggc tgttggtcgc
    19081 cccgctggcg gtgatggtga tcgccccatt gtacgtgccc ggtgtcagcg acgaggtcgt
    19141 gtaattcacc gtgatggtgt ccgtctcgcc cgtgctggta ccgctggtcg gcgtcaccga
    19201 tagccagttc atgttgtccg tgatcgagta gctcagcgtt ccgccgccgc tattgcggac
    19261 cgtgaagctc tggctggcgg cgttgctgcc ctgcgtgcat gacggcgaca gcgtcgccgg
    19321 cgaccgctcg atagttggtg gggcaggcat ggatgtgaat tggccgaact cgatcccgtc
    19381 ctcatagttg tcgcacgttg aagtgacctt gtagtaataa gtggtcccag gcgataggcc
    19441 gatcagcgtt atcgcgtggt cggttacctc tgcgctatcg taagtgtagg agccgtagtc
    19501 cgtcgtcgtg ccgtagtcca cgcgcgaggt gctggccacc tccgtggtcc aggagacact
    19561 ggcagacgtc tgcccgacgt ctccgacgtc gatgtcgctg acggtgatcg gcggcggggg
    19621 cgcgttgacg atctttatct ccctcacgcc aatgttgttc tgcagcccgg tgttgcccga
    19681 gctttcgtgg cagtcgactt ccaccaggat ctgatcgtag agctcggtga gggggcacgc
    19741 cgtccggaac atgcacccgt cgtacagtgt cacgttctgg aatccagcga ggacatttgg
    19801 accgacagag agccagacgc tggcgttcgt gacctcactg tcgacataga tcttaacgct
    19861 gatgctctcg accacgtaac ttgcttccaa atcgcagggg catagagcga aacgcaaata
    19921 aggacccttc caataatcgg ggtcgggtga ggtgtccaga cgcgcggcga cgtaggtctc
    19981 ggggtcgccg tctaagacgt tgttgaactc tcccacgacc ggtgtcccat caggaaaaac
    20041 caacaactgg gaaccacacc attctgatgg tggtgtacac ccggtgtact ctaccagttc
    20101 gtcgaatgca aaattcaggt atcggcgcat gaatccggtc tttttccacc tcagtgtgat
    20161 atcagtaggg tggggccata aaccgtccgg ttccaccaag tcgtagaagg tgccttgggg
    20221 attgccatcg atgtatatca tcgggacgcg gtaggcataa tagcaggcgg cacgcagtcg
    20281 gtttggggcc cacgtagagc gtagctcgga gacgtggtcg agaatgccgc ggatggaata
    20341 ttcatggtag gaggtggtgt caccgtcagc gctggtccag gagttgtatt cgtcgatgat
    20401 gtactccgtg tcatgcgcgt acgcgattgg atacgaaccg tcatcatgca ccgtggtctc
    20461 gatcgtggat atatactgtg aaaactggtt catgatcggg tgctcttcgc agtctcccga
    20521 tgaactttcg tatccgtcgg gatatccgtg tatcgcaaac cccgggagga gcgagtcagg
    20581 gtcagggaca tctccgaact tcccccggta ggccacgtcc accgcctttg cgtagtcccg
    20641 caacttgtcc tgccacggct ggccagccgt cgacggggag tgcggcgtca acggcgcgac
    20701 caggacttgg catttcgtag gacctccgag ttcattctcg tcccgggcca tgcccgcgaa
    20761 gcaaccggac cacacgctgg cgtaccaata gggggggact atcccgccct ccgtgtacgg
    20821 gggactgccc ccgtactcgg cggagtgatt gagttcgttg ccgatgatga accaccgaca
    20881 ttcgttccgc agtgaacggg caagctgcgt acaccagttg atgtaatcag tacgcatcaa
    20941 aagagccatg tcctgatagc ctgggtcctc gggattggcg cctctcagac tgtcgtacgt
    21001 ctcagtgtcc actggcacgg accagtgctg tccccggggt accatatccc aatcgggagg
    21061 ccagccctcc accgaatttc ctgtgttgtc cagccagtgc aggcgcagga tgggtatcag
    21121 tctaaggtgt tgatacgcga aagctagggc gctctgaagc tcgatccacc ttggatccga
    21181 cgaatttgtt gggaatttac cgccggtcgg tttccattca tgcacgtaga acacgatcca
    21241 gccgcgtcgt tcgcctagct gatcgcagaa gatctgcgcg atgggcggct tcatgtgcgc
    21301 gtcgtcggga ttagtccacc aatcggggtt cttccaggag accagatcag ggtagtaggc
    21361 gaacagcccg acatgggaat ctgactcgtg gccgaaggga aaaatgtcag gcgcttgtcc
    21421 ctcgctggcc ctcggcacgg ccgcgagcca tgagacggcc aaggccgtac acgcgaccac
    21481 cttgcctagt gcttctcttg tcataccttc cattgctcct ttctgctact tcgtatgggg
    21541 ttgcgtcaca cgcagccgtt cccacatgtc ccggtcggcg cgcgacctga tgataccgtc
    21601 gatcttgtaa cacaatcggt tgtatgatat aatacaaccg gttttataga ttacacgatg
    21661 ttttgcgcgg ctgcaagcga aatctcaccg ggaagccgct ttttttgcga cggcgtcggc
    21721 gtgggttgcc tgatgcgtgc ttcggcgagg ttgtgggcgg tgcgactgcg aggcagatat
    21781 ataacgtagt aatgtattgg gtacacctgt aatgacacat caggtatatc tttcacgact
    21841 cgcggccgtg tcgtttccgg ttgtggagag ggtcggattc agtggcaagt cgatgtcgtg
    21901 gcacgacttg gatggtgggc gtatgattcg cccgtagtga aagcggggtt gcaggttgca
    21961 gaaacgcaga gatgtcccga cgcttatgga tgtggcccga cgggcggggg tgtcgccgtc
    22021 gacggcctcg ttcatcgtgc gacagcgcga gccgcagttc tcgcagtacg cgtccgagac
    22081 cgtcaggcgg gttcaactcg ccgccaagga actcggctac cggcccaatg tgatggccgt
    22141 gtcgctaggg cggcgccaaa gcccgttctt tggcgtatcg tttcaattca tcagcgacgt
    22201 gatcgccgat cccatgaacg tccgccccat gctcatgtgg gagatcttca ggggcatcac
    22261 cctggccggc caagcccacg cccgctatcc ggtcctgctc accagtcctg atgccgagtc
    22321 gtacatgcgc gacccgggtg cgatcgacca tgtcatacgc tcgggtttgt gtggcatgat
    22381 cgtgtcggtc gatccggcac tgtggggtgc gtatgtgagt cgatgggagg acgccgggat
    22441 accgtgcatc agcattttcg atcgcggtgt ggcaggtggc ccgcggtggt tcgttgatct
    22501 ggacaaccgt cgtgtgggca gcctggcgtg ggagtacctc tacggaaagg gtcaccggaa
    22561 ggtcttgtgt gtttgggatc atcgaaaggc tgccggcgtc gtggatcgca tccgtggttt
    22621 ccgtgaggca cagcgggccg gcggttatcg gtcgatatgc ctgcgactgg ccaggcggga
    22681 tcccgaaacc caagaggacg tccagcacga cgaggccttg ctgatcgagg caatcagaag
    22741 caccagggca acggccatct tcgccacgtc gggcggcgtg agcatggacg tccttgcggc
    22801 tatgtcgcgg agggggctgc gcgttccgga ggattgttct gtcatcggta tcgacccgct
    22861 gttcacgtat gcggagtttg cgcatctggc tggacgcatg accgagatca tctgcccagg
    22921 ccacgaactc ggacgcgcgg cggccaacct gctggcccgc cgagttgacg gtgaagtgag
    22981 tgagcccacc ggcgtcctcg tccaacccga gatcatcgag cgagagagtg tcgcggtgat
    23041 ccgatagagc ttattacagg gggaggggaa cgagtgcacg gccgcgaacc gaacgtcaag
    23101 cggtcggccc gcctgccctt cgggaaaggc tgctgaagca gcccgttccc ggctcttttc
    23161 cgctgccgag ccggaggatt gcgaggagga ccgccgatcc tgctgctgac ctgaatccct
    23221 gtcggggcct tgcggggtga ggattcgcgt cgtcgttggc gttacaatgc ggcacgtgtt
    23281 ttcgcggtac tcatcggagc gcatgtccat gatcgaattg accgacgccc agaaggccga
    23341 ctacttccgg cgcagctaca ccgcagtcga cggcctgtgg ttcatgaaga tggaggagcg
    23401 gtatggcttc gatccggctc tggatgtgga cgaggaggtc tggcgggtca tgcccaagat
    23461 tcaggcgcgc aagctcaaat cgctgaccgg cctggagtcg gggatggagg cgctttgcga
    23521 gtgcttcacg accaagctga cggtcgaggg gtttgatttc caggcgagcc gcgacgcggg
    23581 cggcgggatg accatctcga tggcccgttg cccctggtac gatctcctgg cagaatcgaa
    23641 ccgccgccac ctggcgggaa gggttggtca ccgcatttgc gataccgagt accgcgtgtg
    23701 ggcgaccgag ttcgggccgg gcatcgtcgt cgaattcggc gagcggatct gtgacggctg
    23761 ctcgcgctgc gtggtgcggt tcaagccgtg ctcgtagggc aacgtcgagg tcgtcgccgg
    23821 cgaacgagac tttccgtatg gtcaattccc cggcggcggg tctccaccgc ctcccaccgc
    23881 cccagatatg ccctgctttt tcagatgtga actgcgtcgt cgcaagcggg ccggcagggc
    23941 gagtttcatg cccaacaggc cgaatacggc cagggtcagc ggcgccgaac cgatcagacc
    24001 gcataggtgc cgggtcgagc ccggcgtgta tcgtggcatg gccatcgcat tgtttgtctc
    24061 gttcacttcc ttgaccgcgt ccgtgtcgtc caagcgggct ttcaggacga aatcggcgcc
    24121 cggcagcacc gtcgcgggca cggtgacacg gaaggtcttg gtgtgcagac catgagtctt
    24181 gtccgccggg ttgctgatcg tgtggcgctc gattcggatg tcgccctcgc tgatcgcgtt
    24241 gctgttatcg acggacgcgt agaaggcgat gacgaagtct tcgctgaccg gcatgtcgct
    24301 cagcgagtag gagattctga cgttgaaaat gcgctcccgt ggtgcccgca cgtgttcgag
    24361 tcccgacagc accagatcca ccgggtaatt catgctcgcg gaccggagat tgtcgtcgac
    24421 gggaatgctt tccgcccact cctgctccgg attcaactga gccgtcagca ggaccatccc
    24481 gtctgatgcg atcggcaccg catcgaacgc cgacaacaga ctgatgctga cggcgtggac
    24541 gccgggggtc aggtcggcgt cctcgggtct gccctcagcg atggtcgtat cgatatcccc
    24601 gtcgccgtcg tcatccagac cgatgcgaat ggcgaaggag ggaacatcgg ccgggctgtc
    24661 gatcacgtag gtgacctccg ccgtcggctc gtcgccgcgg ggtttgagtc tcagcagttc
    24721 gttgaccaga ttgacggtcg gcgtcgtcgt gtcgacgttg ttgttctcgc tcgtctcggc
    24781 gatggagttg ccggaatcga gatgcacgaa cacgcggtcg ccgtactgga aacgcccgtt
    24841 aagctgcgag cggatatcgc ccagcatgat cgtatggttt ccaggcgtcc gatagaacgt
    24901 gtcggatgcg acgatgctgc cgagcacttc gtcgacggcc tcgccatcgg tcgtgtcacg
    24961 tctcagaccc catgaaatcg tgaaaggctc gacgctcgag agaccggaaa ccgtatagga
    25021 gaacgacagc attgtctcgg tctcgccgat cagcagcgag atgctgtttc cggaaaggtc
    25081 cgtgcccgcg atgacggtga aggggttcga ggtcgcgccg gacagccccg gcgcgctcgc
    25141 tgcaaaacgg tatccgtcgc aaggttgcgg aataatgacg ctgaacgagg ccacccccga
    25201 aaaacccgaa accgtcgtga cgccctcgat ctcccttccg cagccggacg gcgacaccag
    25261 cgagagagtc acgtcgccca ggaagaacct tctgttgccg atgccgtccg ttacctcgac
    25321 ggcgacgtgg atcggcgtac cgatgtcgct ggtcgtcggt tgttgcgaga accgcaagca
    25381 ctgcgccggc cccgccgaaa cgctgaattg ttccgagacc ccttcggcga gcacgtctcc
    25441 ggcgatgctt gatgctttga ggctgatggt ttcagcgtag ttgtaataga gcccggagat
    25501 ggtaactctg ccgttaccgg cggtgatgac gccggttgac gtgccgctga gactgccgct
    25561 tccgctgttg atcgtgattt gcacggtcgt gttgctggcg acgctgcctt cgttgccggc
    25621 caggtcgacc gcagccacgg tgacgctgaa ggctgagccg gcggtcgccg aaccgacggg
    25681 ctcgaactgc agtctgaccg gcgggctggg gttcacggtg aaggtattgg agaccccgga
    25741 ggccagagca tttccgctgg tgcggtcgat ccgcagactg acgcccgcct cggcggtgtc
    25801 ataggtcacg ctgaagacga tggaactgtc gcccgtgggg atcgttcgcg aaaccgtgcc
    25861 cccgagcgtg cccgttcccg tcgcgaggct caccgtgacg ccggtgtccg tggttacgcc
    25921 gacgatgttg tcgtagtcat caagaacatg ggcggtcaca ataaacgcca cattggctgc
    25981 ttgattgccg atgaggtcca acgccagttt gctgggggcg ccgggagaca cgaggaaggc
    26041 gttggagtcg ccgtcggcca gcacgtcgcc cccggtccgg ctggctcgga taacggcccc
    26101 gatctgcgag gtgttgagtt caacgccgtt gattgtcacc gaactggttc cggcggtgat
    26161 tgttcctgtg gtagttcctt ccagaaggcc gctgccgaga aaaagctgaa ggctgatgtc
    26221 ggtgtccgcc gtcactttcg actcattgcc gaagccgtca atggctttga ccgtcacgct
    26281 gaagggaacg ccggcggtct ggttggcgat gtcgctgatc cgcagggcca cgggcgttgc
    26341 tcccgtgacc gtgatcggat cggacgtgcc ggcaaccagg aagttgccgc tgaggcggtc
    26401 gatcaccagc acgacgttgc tctcggccac gtcgtaggtc acgccgctga tggtcgtgcc
    26461 gctgttgccg gtggtgatcg tgccgtcgat gttcccgccc agaacccccg ttcccgccga
    26521 gagcctcagc ctgatattgc agtcttgggt gacgttcgtc agattctcga attggtccgt
    26581 gacgtagatg tcaacgtcga acggtacgcc ggcaacgggg atgccaggcg gcgtgatgtg
    26641 gagcaacagc ggaaaccatg gattccactg catggccgca ctggtgccga cggccggggt
    26701 gaccgcatcg ttagcggtga cggtaatgga ctcggctcgc cgattcgtga tgtcgagcaa
    26761 gatcttgcca tcggcgtcga aagtcgtccc aacccccagg tcggccgtgc cgtccaggtg
    26821 atcggtcagc gtggccgggc cgttgtccca gtcgatgttg gtgccgtcgc cgcccgtggt
    26881 cgaggtcagg ttcacggttt gaagcgtcac gtagttggga atggggttgc tgtaaacgtc
    26941 gcgggccgtg atggtcagcg taatggggtt attcacggtg accgggttgg cgccgggcac
    27001 gacggtgaac gaactcagca cggggtaggc ggtgatgttg aatgcgttgc tgtccacgtt
    27061 gtcggagccg ctgaagacgc cgctgccgct ggcccgaagg gtgtagccgg ttgcggcagt
    27121 ggtgatgttc aaggcttgca cggccgtcca cgtggctacc ccgtcgacgc tgagcagcgc
    27181 ggcggttccg ttgagaaccg cgccgccggg gttggtcgag atcgtcaagg tgatcgtgcg
    27241 attgtccgta tcgcaggtgt tgtcataggc gtcttgtatg gtcaccgccg gcacgaggtt
    27301 ttgtcccgca atggtatggg cgggctgcac cgtgaaagcc aggtggtgtg gcacgttgtg
    27361 cgaaatatcg aaatcggcgc tgtcagccgt gtcggaaccg gcaaaagccg cgccgctgtg
    27421 cgacgcgcgc aggtagtagt tgacgcccgg ctttgtgacg ttcatggctt ggccggcggt
    27481 ccattgggcc acaccgccgg tggtcaggag cgaggtggtg ccgttcagcg tcgcaccgcc
    27541 gccgttcatg agcaccaacg tgatggttcg ctcatcgccg gtgaccgtgt tgttgtacgt
    27601 gtcttgtatg gtcaccgccg gcagcaggga ctcgcccgca agactgttga ccggctcggc
    27661 gctgaaagcc agatgatggg ccgctgtggc cgtgatgctg aaagcgtcgg agtccgccgt
    27721 gtcagagccc gcaaaagccg ccccgtcgtg tgaggcccgt agctgatagc cgttcgccgc
    27781 aacggtgatg ttcagaccgt ctccggctcc ccagctcgcg acaccgccca ccgtgacttt
    27841 ggaaacgttc ccgttgagga cggctccacc cggattggcc gcaaatgtca gcgtaatcgt
    27901 tcggtcgtca ccggtgacgg tgttgtcgta ttcgtcctga atggtcaccg cgggaagcag
    27961 attggcccct gcggccgtcg ggctgaccgg ttggatcgtg aacccgagat gatgcgcagc
    28021 tccgcctgtg atattgaagg cgttgctcac gacggtgtcg gacgtggcga aggccgcccc
    28081 gtcgtgagaa gcccgcaact gatagccgtt ggcggccacc gtgatgttca tgagttcggc
    28141 ggcggtccac gtggcgacgc cgttgaccga tggcagcgtc accgttccgt tcagcgtggc
    28201 accgccgggg ttcgctgcga agtccagagt gatgttgcgg tcgtcggtcg tgcaggtgtt
    28261 gttgtaaacg tcctgaatgg tgatgaccgg tacgatagcc gcaccggcag cggtggtgac
    28321 cggttgagtg gtgaactcca tgtggtccgc cacgttatga gtgatgtcga aagcggcact
    28381 gtcgacggtg tcgccccccg gcagaagggg ggcggtgaga ttggtagccc gaagctggta
    28441 acccaccgcc ggtacggtga tgtccaggct ttcgaccgcc gtccacgtgg cgacgcccga
    28501 tgaggtcgtc ttggtaaccg tgccgttgag gctcgccccg cctgggttcg taatgatcga
    28561 gagctgaatg gatcgattgg gcgccgcagt gcagtcgttg tcatagatat cccggattct
    28621 caccgccggc agcagatctt cgccggccat ggcgttgacc ggttccgtgc tgaaggccag
    28681 gtggtgatgg gcggccggcg tgatgttgaa cgtgttgctt tgcacggttt gtgatggccc
    28741 tgccagggcg ggcccgtcat gagaggcgag aagcctgtag cccgttccgg ttttggtgat
    28801 attcatcgcc tcaacggccg tccaggcggc aaggccgctg ctggtcgcca ccgtgacggt
    28861 accgttgagt gtcgttcccg agggattgac cgagatggtg agggtgatgt tgcgtgatgg
    28921 gtccgtcgtc acgacgttgt tgttcacgtc ctgaacggcg accgtcggtg tgagcgtgac
    28981 cccggctgtc gagctcgcgg gttgaacgct gaagaccagc ttgtaaggcg ttccgggtgt
    29041 gacggtaaac aaattcgaca cggcgggtgc gagcagatcg ccggccgtcg ctgttgcgcg
    29101 aacctgcttg ccggttccgg ccgtggtcca tgttgccgca gggtaggtgg tcgaagtcgt
    29161 ccccgcggtg atggtccgag taccgccgcc gccgagtgcg cccgtgccgg cttctcgcgt
    29221 gagcgatacg gtggtgttga ccgtcacagg tgtgggaata ccacctccgt caacggccgt
    29281 aacaatgact tggaatggaa cgccagacac ttgatccggg atgttggtga tttgcagagc
    29341 agttgcctgg gcaaagaccg caggcgcgca cagcagcgtt acgacaatcg ccaatgatgc
    29401 acagcgtccg ggcgcaagcg gcgcttgccg ttcgtcggga ttccaggacg tgaggttgtt
    29461 cgattccacg gtcatcaggc tgtcaacaag ctcgatacgc ccgacgattc tcctttcgct
    29521 ttggtcatac tcttcttgag cgctcgtgcc agcgcacgcc gcccgtccct tcttcctcgg
    29581 tggacgggct gaacttgccg cggcagtgca agattccccg gcgataacgc cccccggagt
    29641 cttttgatgc gttttcttgc tcgccgcgtt tgccgtgagg tccgaagatc tctcgattca
    29701 ggattcttcg caacctcttg caaacatgat atttagttat cgacacgtgg gctcgcgcac
    29761 ttgccctagc atgcttgggg taaccctggt tctgacaaac ccgttctgat ctgccccctg
    29821 gatcaattcc ccttgggtga cggctatctg tctcacaaaa cgcggatagt agaacatccg
    29881 gcaagtttcc tgcgcggcgg aatagcaggt ataagctttt ccaaagcaat gcgttaagtc
    29941 gctttggcgg tcgccaggac cgggttgctt gctgctcggg gtcgctgcgg gtctgtggaa
    30001 tcctctggtg gccgcatccg caatcgaact cgcgccgagg tgtgcgtatg ttatggggcg
    30061 taggtgatga gactgcgtgg cttgtacgga gaccctgaga tgcggcttgg caaatcacgg
    30121 catatcacgg ggttatgccg ccgagaaggt tttctgccat gggtgttacg tcgccgatgc
    30181 tgctggtcat gcatgccggc cctgatgacg actttcgtgt ttcttgaggt tgctcgggcc
    30241 ggcccggtgc ctccctcaga gagtatcgcc gcgacgcggc ctgtttcacg gatgggtgac
    30301 ccttggggga tgatctacgg cgggtccgga tggtgggagc acaacaatcc ccatcggacc
    30361 aacatcgccg aacgcgcggc ggccggggcc aaggaaatgg ccggatgggg cgtgcaggcc
    30421 tcgcgcgtgg gtatctgctg gcacgacgtt gaacgagaac gggggacgta tgattggacg
    30481 atgcccgacg cggtaatccc cgcggttgcc aaggccggta ttcatgtcgt gctctgcgtg
    30541 gccaccacgc cgcgatgggc gtggcagcac cccggcgtcg aggcgattct gattgaacgc
    30601 gggcaggaaa acctcgccgg gtgtcttccg cataagccgg agttctggcc ggactacgaa
    30661 cgctacctga ccgaggtcgt caagcgctac ggcgagcttg tgaagcacta tgagatctgg
    30721 aacgaacccg acggactggc cggctttcgt ttcatggagc ccgagccggg gcatccgatc
    30781 ggttttcagc acggcggcga cccgctctgg tatgccgaac tggtcaaacg cagctatcgg
    30841 atcatcaagt cgcttgatcc tcaagctcgc gtcggcatcg gcagctacga gtacaagaag
    30901 gcaatcgaac cctttgtcct cgacgcgtca atggccgctt tgttggtgga aggccgtgtg
    30961 tccgacgcag tccgcagggc gttcggcgag catccggatg cgccggtctc gacttcggcg
    31021 acgatcagca tcgaggtgcc cgatcgccgg tggatcctga atgatgatcg taacggccga
    31081 cgatatcgta tcgtgagaga gaacgaaggc ctcaaggtga ccgatcgaaa aacgtggttc
    31141 atcgagcgat tgtacgagga aggcgtcaaa gatcattgcg acgccatgag cattcacccg
    31201 tacggcaacc ccttcggacg cgagtggctc ggcaccgttc ggcggctgat ggtcgaacac
    31261 ggcaatgcgg acaagcctct gtgggtcaac gagtacagcc ttcacggctc gggcaccagc
    31321 ctggcgttca acacggtccg ccaactgcgc ctgcttcgcg aaacaccgtg gatcagcatg
    31381 gcccagcccc tggtgtttgc cagtcatctt gacaagaaca tcagtccgag cggacgttcg
    31441 ttgcgggccc acaagagaat ggcggaagag tataggcctc gccgtgagat ggtgtttgat
    31501 ttcgagggcc cggtgctcaa gctgctcgac gagtgggaat gggacgcgac cggtgcgggg
    31561 aaagacgacg tcgacgagcc ggagctcagg caggagttcc cgcacggagg cgaacggtgc
    31621 ctcactgctt caacgccgga gaaaagggtt cgcgtgtggt tcagccccta tgtgaaggcc
    31681 caggaccggt ggttcaaggc ttttgtcctg gccgagccgc gggaaaagga tgctcagctt
    31741 tcactgtcca taggtgtcga gagcggcgac attctgcaga accttcgtga agtccgccca
    31801 ttcatggccg ccgtaccggc cgagcagtgg ttcgaggtga gttttccgat cgatggaagt
    31861 ttccccgaat ggaaggattt gaccacagtg acgatctatg ttgagattga ctcggacaag
    31921 ccggggctga gggtatcggt ggatgacgtt cgcgtgggca gcggccgaac ggtggcgtca
    31981 gagcggtagg agacagccgg gatgtcgaaa aagaatgccc ttatcgggag attgctcttg
    32041 gcgggttccc tggtcgtggt cgttgagcgt ccgtgcccgg ctcaggccac gcagccgacg
    32101 acgcctgctc ccgtctttgt ccccgcgatt gaggggcaga agcgcgtcct cgccgagttg
    32161 ggcagtatcc tccaggaaga gaaggtgatg ctctcggacg gggcggttca gttggcgctg
    32221 gagaccctgt tgagcgatga acagaagcag gccgaggcta ttcgcgagtt gggcaggcgg
    32281 accctgggcc gaacagcgga ggaactgagc gcggaggatc gagccgagcg agaccgcctg
    32341 tttgacggcc aggacatcct gaccgatcgt taccgccgcc tcgaaaaggg gatggagaat
    32401 aaagcgacca tcaagcccga ctccgtctac accggtctgt tggccaaggc tgcagattct
    32461 cagttgctcc agaccatgga agacgccggc aggcaggtcc gtgcgaacca gctcagcaac
    32521 gccttgcgct ccaccaacaa ggtcatcgag gtgctgcgcg agatggtcgc aatggcctcg
    32581 caaaaggccg gtagcacgca agacatggac aacacgaacg gcgacggggg tttcatggcc
    32641 ccttcgctcc gctacaaggg ggctgctgag aaactggccg agttcaactg gatcggtgcg
    32701 tccgccgacc cgctgggaca cattcttcgc ggcctgcggc tcttggagga actgaccgaa
    32761 cgacaacgcg ccgtggctgc ggcaaccaag acccaggcgg cctcaaaaca ggcgtcggcc
    32821 gatctggcga agacggaaac cgacattcgg cgtcaggccc ttgaagtcgg cgcaaagctg
    32881 gccttgctcg atccgcagat cgatcagctt atccgcaagg cgaccgcctc gatcgacaag
    32941 gccagacccg cgatggagca ggggccgctt tcggatgcgg tggaacccgc caggcgggcc
    33001 gctgatgagc ttgcggctgc aaccgagtat ctccgcaagc tgtggcagga cattctctcc
    33061 cgtctcaagc aacatacgca ggaagccgcg gccatcgagg gtgcaggcgg cggcatcccg
    33121 catggcatgt ccaaggccca ggtcgagcag atccagaaaa tggtgaccat gctccttcgg
    33181 gcgacggcgg cgctggcccg cgtggccgat gctcagacgg ccatactgga tcgtaccagg
    33241 ttggccgcaa gcgagcccga attaccttca ctgaagcccg atcaggaagc cttggccggc
    33301 gcgatcaaca acgacgtggt cccgtttgac gtcagcgcgt cgcaattgcc cagggatatg
    33361 ttgcctcagg gagtcgccgc gagcgtcaac ccggtgggag atctgcttaa ggatgccgtt
    33421 gatcgaatcg gtaaggcagc ggatgctctt agcaagcccg accgtgccga ggcggtcaag
    33481 cgggaaagtg agtctttgca ggccatgacc acggccatgg acctgatgat caaagcgctt
    33541 cagaaactgc ttggccagct tgctccttcg gcgatcacgc caagcggtcc gggagccgcc
    33601 ggaatggccc tcgatcccag cggcggcggc cggcaggccg gcgggtggct gttcggcttg
    33661 ccttcgcaac aacaggagag cgtccgccag gcctttcgcg agaccttccc ccgtcgctat
    33721 gatcgtgcca tcaagttgta ctaccaatcg atcgcgcggg agcaggcatc agacggcaac
    33781 caatagcgga ttcgcctgaa acgtgtgtgc aagaacggac ggagccggtt ggtacggcgg
    33841 cgtcgaggtc gcctcggtga cggagacctg ccatattctg cggccaggag tgcaatgtgg
    33901 ccgcttcgtg atctggtccc ttttgaaagg aaggtccctc gctcgcggtc gccacgtcga
    33961 ccaggctcgg ggttctgaaa gagctgccca cagcggcacg ggcccggtgc caagcgctac
    34021 tcccgttccg gagccatcag cgagtggaag aagtccataa tggtcctttc cttgtagtcc
    34081 ttgaactccc cggcgtcgaa gaatgccccg tggcacaccg tgcagctttc ataccagata
    34141 tgtggctgct taaggtcgac cattctgatc atctgggtat ggcacaccgg gcagttgatc
    34201 ttgcccacct tgttgagttt ttcagctcgg tccgggtctc cggtgtcgat tcgctccgaa
    34261 cctctttgtg ccttcagctt ctccctctcg ccgacgtcga accagagtcc gccgcagtcg
    34321 gtgcagcggt cgacttcgac accgccgtac gtaaccttct ccatggacga gctgcatttg
    34381 ggacactgca tgatcaagcc tcctcataag caacagtggt ggcacgtgct tccagagaat
    34441 agcctgctac ccgccggcat gcaaccacaa gtgctcttgg ggttggggat ccctgccaac
    34501 ttgcggtgtg tcactgccaa cttgcagcgt cccgttgcca acccccgggt cgccatcggc
    34561 ggcttgtttg ccggcgcatg gaacgaaccg gacgaaaacg acaccggccg ccacgttcac
    34621 accgcgatca gcccgagtat gccgaagatc acgaaaagaa tgcccgccgg tatcaggtcc
    34681 cggcgcttga agggcggctt ctctgtggcg aacggcctgg ttgcgaccgt tccgactccg
    34741 aaaacgatca ggatgcatcc gacgatatca aagccccccg aatcgcgaag cacgatcaga
    34801 atgcccgtcg aaatgagcag caaaatcgcc agcccggcca cggcgtcgcc gatactcgcg
    34861 cgcatcagcc cccgcaccca gaaaaacagg ccgatcatcg gaagcacaat catcaagatg
    34921 acgggggcaa tgttacacat tctgtcttca gtcgcttggg gccgtcgttc tgccggccgc
    34981 ttttgtctcg caacagtgta tccgattcct gatcccggtg cgagaacaaa agccggcgta
    35041 acctctgaga aagcgtggtc ctatcggttg ggcatcggcg cggtttgcca aggccggaaa
    35101 gtaggtgcgc cgaggatgat cgccgcaact acctctggta aatcggcagg taacggcagg
    35161 ggatggacaa cgtcagcacc atcatggtcg tcccgtaagc cggtccgtgt tcggagtccc
    35221 agtatgcccc ctctctctgt tgtgccagca cctgctcgcg gatcggcgga aaccattgtt
    35281 gccagcggcg gggatccttc gcctggtaca cggcctgggc ggcatagtag atcccgtagt
    35341 ggtagttgcc ctccctttcc ttgtccgcgc cgaggttggc gatgagatag tccaggcctc
    35401 gccgcgtttc gtcgcagtcg aactcgccgc agacctccag cgatgtgacc cccgcccccg
    35461 tgcgggcata acccggcttg ccggaccgcg tctgatagta gaagccgccg ccgcggtcgg
    35521 cgcaaagctt gacgtatttg attgcacgct cgatggtctc ggccggcacg atcatgcccg
    35581 catgctgtgc gccgcgcagg gccaggatct gcatgacggt gaccgaaata tccgcgtcgc
    35641 ggctgtgagg ttgatatcgc cagccaccct cgctgttctg agcgttcacg atgacccgca
    35701 cggccgcctg gatcgccgcc gcaatctccg gtcgctcgct catgccgtaa atctcgctga
    35761 gcatcagcgt ggccgccgcg tgctcataca tggggccgtg tgaggacccg tcggcgtaat
    35821 ggatgaaacc gctgggctga gcattggcca acacccaacc gatcccgcgg tccagcaccg
    35881 gcgcgtattc gccttgctcg ggaacgttgc cggttgccag aaaagccaga atggccagcg
    35941 aggtcacccc cacgttcgga ccgaccttgc cgtacatcca cgccccgctg ggcaactggc
    36001 accctgccag aaacttcaac gcgctgcgga tcgcctcttc cgatccgggt gtcatctcca
    36061 cgaacatgtc gggctgttca ggcgactgga ccgacttctc agcctgcgcc acgaccgttc
    36121 gcggcggcag aagcgtcgtc aacgtcagaa acaggatcat caggcgtccg ttgctcatca
    36181 gcgcgcctcc gcaggtcgtg tcgccggtgg ctgctcgccg cgaaacgagt tcaaggcatc
    36241 ctcgagggcc agcacgatcg ccggcatgtc cgtgccgccc agtcgcgcaa gccgctcggc
    36301 cgtcgatcgc agtcgcagcc atcgctcctg ctggccaatg cgcgacgcgg cgtagtcatc
    36361 ggccacccgg cctatcgact cggcaagctg accggcggtt cgggccagac gatcctgtcg
    36421 ccatttctcg gctcgcgtcc gttcctgccc ggccgtcgat tgtcccgact gctcgccgct
    36481 tgcgattcgg ttgaccgctt ctgcacattc ggcctggagc gattccagtt gcgagagctt
    36541 gtccatgagc tgttcgagct cgtcattcag cgctctggcc agttcaactt cgctcatgat
    36601 cctgagtctc cgcgaccggg agacgggcca ttcctgtcgg gcggcgtgtt cgccttcatc
    36661 gcgggcctcg agccaccagg tgagcgagtc cccgggtttg aggtccagcg gctccagtcg
    36721 ccagtcgtat tgcacggcgg cacgcttgcc ggatctgttc agcgggagct cgatacggcc
    36781 ggtatcagtc gccgttcctt tgcgatacag cagtgtgacc atggccgctc cgtaatcgtc
    36841 actgatctcc gcggagatgg gtagcacggc cgtcggagtt gcccggcggt cgatggacgg
    36901 cgtaacccgg tcaaactgcg gatactgatt gtccgtcatc tcaatgcggt agacgaccgg
    36961 atcggggttg tggaagccgt ccgcgtcggt caggtggacg cggtacgact ggctgctccg
    37021 cacgaccaag gtcgtctccg cgcccggcgc gggcgtcagc gtcatctgtg ccgaggtgcc
    37081 gtcctcccag gccagtcggc cgtcggtgat ggacttgctg gcgatgacga tgagcctgag
    37141 ttgcgtcccc aagatgacct ggacgttgcc gtcctcgatc tgttgcgggg gcagatgcgt
    37201 gtactcgggg taggtgcatt ccgttcggat cgcgacaatc tccggcggcg gaatcatggc
    37261 cacccggctg ggggacgtgg cgttgtcgcc gatctccagc cagatctcga tgtcctgcat
    37321 caccggccga tactggacgc ggaaatcacc gttttcttca atctcgaaat atgctcgatc
    37381 tgccgggctg cgtccggccc gcacgcgcag cgttccgctc gacgggactg cgccggtcac
    37441 ccggccggcg acaatcagcg ttccgccccg gaccacgcgc tcactgatgc tcgatacgcg
    37501 tatgtgggtt cgtttcggcc agtcgacctg agcccacggc agcagcaccc gcctgatgcc
    37561 gatggaagcc gactcgcggt tgaatcccgc gagggcgagg acgatggcga ctccaagcca
    37621 tgccgccgca cacctcttga cgatcgattc tccgggtatc acggcctttg tggccaacag
    37681 tggtgccgtg gtctcggcgg cggtgcggac ccggccatag aggtcgtctg acgcgccgct
    37741 ccctgcccgg cgggatgcat ccgccagttg aaccgcggtc acaagtcttc cgcgaagatc
    37801 cgggtaggcc ctctcgatca tgagggcggc cgaatcgaac gacaggctct tccacaaggg
    37861 catgatgctg cgacgcaaaa cccacacaac ggatgtgcca agcagcacga gcagcagcgc
    37921 ccgccaccag cgcggaccgc gaatccacgc atcgaggagc actgcggcaa tcatggtacc
    37981 gctcaaccaa acagccagcg tgctgaccgc cagagtccgc ctgagccgcg cccagcggcg
    38041 ccgcacttgt acaatccgaa aaaggcaggc cttcggggtc gcaagctgag gtccgtctct
    38101 cacggtttgt accgctgctg tgctcattgg atcatcacgg caactgccaa agccttcgca
    38161 gaacccaatc cgctgccaga aggctcacaa gcatcaaaac aaggatcggc ttgtcccaca
    38221 gccgatagtc tcgttgttcg ccgaccgaac gcggagaaag atcgattccc gagatcactc
    38281 tgtcgatgtc cgaggccaag ccgctttgcc caccagtgtt ctcggcgatt tccctcatca
    38341 gtgccgggtt ggccgccggt tccgccggct cgagttgcgg cgcctggacc tccacaatcg
    38401 acgaagcctc aagggcgtaa ccatccagcc acagcttgtg cagccccgca acggtcagcg
    38461 gcacacgggc ttcgtacatg tgttcggcgc cgggcgaggg agtcagtggc accttctgct
    38521 gacggccgtc ttcgctctcg gttaccagca ccggcagttc cgtcccggcc agttcctcgg
    38581 cccgcaccgt gaccagcgcc gtgtcgttgc gattgtacac gcttcgatcg gtccacagat
    38641 gcacctggcg gctctctccg ccgaggtggg gggcgccaag gtattgaacg acctggcccc
    38701 agagtcgata gaaaacgcgg tctccctgct tgaaccgcca gcgccacgtc tcgtcaatgc
    38761 cgcagaagaa gctcttgcct ttgccggagt tctgaaccgc catgatcggc atgcgctcat
    38821 tctgtccggc ttgggccgtc tccgtcgggt ggaccgccag caccagtgcc cccggccgtg
    38881 ccttccggac gggggcgaac cagtagacgg ccggcagggc cttccattgc tcgaggttgc
    38941 tttccatatc gtcgccaagc tggatcgccg aatgcgtctg tcccagaggc gtcaattcgg
    39001 gaacgaacag gtcatctttg ccgctcctgg gtctgtgagg cgaacgtcct gcctcctcgg
    39061 ccgcttcgac ggggaacagg tcggcgaatg acgtctcagc ataggcctgc gggttgaagc
    39121 tcggaccggc cagcagcatc atcccggcgc cttcatcgcg gaccatcgcc ccgatccact
    39181 ccgtctgttc ggccgtcaaa atgcccggat caacgtcgcc gatgatgaac acgtcatagt
    39241 tcttgaaggc atcgcggttg ttcggcaggg aggcgatcag cggcggcgga ggattcgcca
    39301 ccggctcggc tcccgtcaac agaatctgta acctgagcct gcggtcgcga ttcatcgcgt
    39361 cgcgcagaaa acgatattgc catcgaggcc ggccttccag gtagagcacg cgaatcttgt
    39421 cggccgtgac tcgggcgacg aagctgcggt ggttgttctg gtgggcggtt tcgtcgggtt
    39481 gcggtgccac ttccacgcgg aacgtgtgtt ccccggcctg acggggcgtg aacgtgatgg
    39541 ccacgttgat ttcgccggcg ttctcgtcga atacggcatc cgcacggccc agttcctgcg
    39601 atccgcttct cagaacgacc ggcacacgcc gcgtctggta gccccgctgt ctgagtcgaa
    39661 cgacgaccgt ggccggatcg tcgctgaaca gcagatcgtt cgccaggacc tgccgcacct
    39721 cgatgtcccg cgggttggcg tcgcctacgg tcacggtgtg tatgggcacg ccggcctgtt
    39781 tccagcgcga gcaggcggcc agcggtgagc cgccggtatt gttggctccg tcgctgaata
    39841 gcagcacccc ggccgtgggc cggcctcgca gtcgctcaac tattccgtcg atgctcggtg
    39901 tgagggcggt atacggtgcc gaagcatcca actcgcccaa gccggccgat tcaccgttga
    39961 tgcccacggg gcgtgccgtc cggtcgaagg taaagaggag tagttcggcc gagcgagata
    40021 accggtccag tcgtgagccc tccctgtctc ggtcggcaag cagcagcgac ttgacaaggt
    40081 cgatacgcga cacgccggca ggcgcggacg ccgacacgac ggcaggcgtg cttgcgggcg
    40141 ccgattcacc cgcgccaccc gtggcgtaac ggtcctggag ggacatgctc tgcgatccgt
    40201 cgacggccac ggccagcgtg ggcttgagtc ggtggctgac ctgaacctgc aatgagagat
    40261 cgagtagcat gggcacgagg caggcgacgg ccgcaattcg aagcgacgcc agtgccgtcc
    40321 gcttgcggag cgacaggtct ggtcccttgg tccgcgagat ccagatcgcc agtatgacca
    40381 acccgattcc ggccgcgatc gttcccgccg tcccaagaag cggataagcg cgcagagaga
    40441 cttcgcttga ctgcccgtca agttgctgag gccacaacag ccagttcaac caatgcggac
    40501 cgctcatctc ctgttacgct ttcgttacgt gttcctacaa gcccatcgct ttcttcagca
    40561 ttgcctgcgc gtccacttgc ctgggagcga acaacttgac cagcagcaat tcagcggcca
    40621 gcaggatcag taatatcacg atgccggtcg tcgacagttc ggcctcacgc ttggccgtga
    40681 gcaaggccgt ccggatcgaa tcgtcggggc cgatgatccg cgtcttcgct aacgacggtt
    40741 tctcccgcaa ttgtgcgagg gcaagcatgg ccggatcgaa ctcatcggca ggcaggttca
    40801 ccgctttctt tgcctgtctc ggagtccccg tcgcatcgtt gtatgtcaaa gtgtagattc
    40861 ccccgcgctg tgccgacggc acaagaacgc gaggctgctc cgtggtcgga tcctcgccga
    40921 ccatggcctc cgcaggcacg cttgtgccgt cggggtaggt gagagtgatg ccgtcggtcc
    40981 tcgcggggtg ccattcaagg gctcggcccg ccggttgctg cggctcatcc tgtccgaacg
    41041 tcaggtggaa ggctagttgc tgaaagaacg gcacaaacgc cggcgaaagt gggaactcgc
    41101 cgccttgccg gtcggccgat ccgccgaagt acaccacgcg gccgcgcccg aaggtgttgg
    41161 ccaccagtgc cgctccgcct cgcgtgaagc gggcccaggt ctgcacgttc ttgccctgcg
    41221 cgaaagccac cggacgcacc gttctaaacg acaccgtcga caacgcctgc atcaggatcg
    41281 ggtcatcaaa cgccgacagg gccggaacga gcggctcatt caacgccaat ccgaccggtg
    41341 gatcggtggg ctgtaagtcg attggcggac cgagttgtac cggcagaagg ccgctgcctc
    41401 cctcgaagaa catggccgca aggttcgagg cgttgacctg tgatcccgcg atgatcagca
    41461 gccccgagcc ctctcgcacc gccgacttga tctgcccgat tgccgtgctc gggagggcgg
    41521 gcacgtccgc caggatgatc agcggaaagt tgccgatttg taccgattcc aggctgccgg
    41581 tcgatagcgt tcggacatcg aagaacggta cgctttcacg ttcctgggga tctgttggcc
    41641 agagagcgat gcggatgaag ccgccggccg acgtcgccgg atcgggcccc ggtcgtccgt
    41701 ccaccagcag cacgttgatt cgctcgcgca ctttgagcag catcgatcgc tggttgttcc
    41761 gctcatacag gtcgctgtca caacgggctg ccacggtgtg ctcgccgggc gagttgaaac
    41821 gatgctcgaa gccgacgatc agatcctggt ccggcggcac gctgacttgt ctgcgggtga
    41881 cctttcggcc gtcaacgaag aggtcaatgg ccgtctccag cgtctctttc gtattgaatg
    41941 cggtcagccg cacgttgatc gatgtgggcc acagggtggc ggccagcgag ggaatgaggg
    42001 cgtcggggcc gcccgagatg gtcaaatcgg tcaccgccgc atcccctgcg ggggtcgggg
    42061 ggacggtgag gacaaagacc tcgcctacct caccggcctt cgcgaaggcc tcccgttttt
    42121 tggcatcatc ctcttgccag ccggctgcct ggccgtcggt tacgagcacg atttcacggt
    42181 tgggcgtctt ctcctcgcgg agccggtcgg ccgcgctgct catcgcctgt tcaatcaccg
    42241 ctgtgctgtc tcgcggggcg agtctcgaca gaatctcgcg cacatagttc atgtcgcgcg
    42301 tgggttgacg aatgacatca accggcggat cggccgcaag gatgaccgcg acgtcgctcc
    42361 ctgcgggtag ttgatccagg tattcctcga ttcggacaac ggctcgatcc atccgtgttc
    42421 gaatgccgtc cgacgccgcc atgctggccg aatcatccag cacgatgatg ctgaccgttc
    42481 gttgccgctg accgctcaac cattgccagc ccgctcgggc caccggccgc aggaccacga
    42541 aaatgatcgc tgcgacgatg gcggtgcgca cgatgagcag caggatcgtc tcaagcagca
    42601 gccgccggcg gttcttgcgc aaggcccgca gcagaaaggt catcgccgcc cagcggacgc
    42661 gcggcaatcg ctgccggatg agaatatgga tcaggaccgg aacggccacc agcggcagca
    42721 gccacagcag ctcgctgttg agcagcttca tcgccggccc tcccggatct tgccacgggc
    42781 ggtcaggtac cggaccagca ggcgatcgac cggctcggcc gtgttcacca gcacgtaatc
    42841 ggatcgcgtg ccggcgcagg ccatcttcgt ctcagcaaca aacttctgga taatgtccac
    42901 gtaatccgcc cggatcttat tcggctcggc gacggccgcg ggcaacgatt ccatcccctc
    42961 aaagcggatc ctgccgccga agtcgaagct tagctcctgc gggtcgagca cgtgcatgac
    43021 gaccacctcg tgccgctgat ggcgaagccg ctgaatgacc cgcaggatgc cggccgtgtc
    43081 gtcctcgccg ccgggagagc tgacatcgag cagatccgag atgatcacga cgatcccgcg
    43141 ccggccgatc atttccacca gcaccggcac caccgtctca agcctggtgc cccgttccgg
    43201 cctgacggtt tccaggcttg atgccacgac cccgagcgag ccgagcgaag ttcgcggagg
    43261 aagaaaggcg atgagatcct catcgaacag ggccaccgat accgcgtcgg actgtcgcaa
    43321 cgccaaatag gagagcgagg cggtcagcag gcaggcatgg tccagcttgc tcgccttgcc
    43381 cgagccgtac cgcatcgact cgctgcagtc caggataaac gtcgccacga agttggtctc
    43441 ttcctggaac tgccggacgt aataccgctc agtccgccca tagaccttcc agtcaacgtg
    43501 ccgcgtgtcg tcgccgggta cgtacgcccg atgttgcgca aactcactgc ttgcaccgag
    43561 gtagggactc cgctgcaagc cggccatcgt gccctcgacc agatccctgg cccgcatctc
    43621 cagcgacgcg atatgatcga gcacgtcgaa aacgacgtat ttgcgatcgg tcagtctttc
    43681 ttcgcgtcgt ccggccattc tgcggcgtcc gccacgtcaa atcgatggct ttgatatacc
    43741 cggccctgtc agaaaacccg aataagggag gagtggtcag tgatcagtat cgtctgcaag
    43801 gaaacgaact gataactgac gactgaaaac tgagcactcg tccttgacat tcggatttcg
    43861 acattcatca ttcctggact cgccctttgc gctctatgga attccagact ctatcctgaa
    43921 ccccgaaccc tgaaccccga accctgcttt tgccccggcg cagctcgcag agcgaagccg
    43981 gaaaccctat tccttctccc ccacatgtgc caacaactgc tcgatgatgt cgtccgtcga
    44041 gatccgctcg gcctgcgcgg caaagttcag aatgatccgg tgtcgcagca ccggcttggc
    44101 caccgcccga atgtcgtccc agcttacatg ggcctcgccg tgcaggattg cccgagcctt
    44161 ggctgccaaa atcaggttca tcgacgcgcg tgggcccgcg ccccaggtga cccacttgct
    44221 gacaaaatcg ggagcgtctg cttcggttac gcgcgtcttg cgcaccagcc gcttggcgta
    44281 ctcgatcatg gcatccgccg ccggcacccg ccgcaccaac tgctggaaag ccaagatccc
    44341 ctcacgatgc agcacgggtt cggctttgaa tgtcgcgggc gaggtcatcc ggcggacgat
    44401 ttcgctttct tcctcatcag tcgggtagcc gactcttacc aggaacatga aacgatcgag
    44461 ctgagcctcg ggtagcgggt acgtgccttc ctgttcgatc gggttttgcg tggccagaac
    44521 gaagaacgga tcgtccagct tgtagtctac gccgccgacg ctgatcttcc gctcctgcat
    44581 ggcctcaagc aacgccgatt gcgtcttggg cggcgtgcgg ttgatctcgt ccgccaggac
    44641 aatgttggcg aagatcggac cgcgaaggaa cttgaatcga cgctcgccgg tggtcgggtc
    44701 gtcctggatc acctcggtgc cggtgatgtc cgtcggcatg aggtcgggcg tgaactggat
    44761 ccgcttgaag gtcagatcca gcgtgtctgc cagcgtgctg accaggagcg tcttggccag
    44821 ccccggcacc cctaccagca ggcagtgccc gcgggcgaag atggtgatca tcagttcctc
    44881 gatcacttgc ttctgtccca cgatgacgcg ctcaagttgt gagagaatgg tctgatagcc
    44941 gctcgaaagc tgtaccgcca attccttatc gctgccggcg aacgttgctg tagaactcac
    45001 cagagtacct ccaagattgc ggggcgtgcg caccggttgg aatgtgtgcg aaatccgacg
    45061 aagccggtcg gtggggctgc gacaaggtcg tagtggcgac ccagacctgc cgttttctgc
    45121 agccccaaac ggctaccgtt ctcacacgcc ttcccgggcc cggctgtctc cttttacgtc
    45181 gatgaatttc agcttcgcaa ctgacggcct agcatatcgg ctcttgcaga acgcgtccat
    45241 tcccgcccag ggttcggact cgcatcttat ttttgtgggg acgggcttgc cgccgggcgg
    45301 ctttcgccct ttcgggggtg ccaccggcgg cttgccgccc cgtgcatcac tcgcttttcg
    45361 agaacgtcac cggtccggcc ctgcaagacg cggattcgta ctctgtgcca aggtgtggca
    45421 ggtgatgccc ttggttcctt ttgtgggatt caacattgag cccgcgtggg ccacacaata
    45481 gaatacctcc cgccggggct ggcggccaat cccgggcttg cgcccaaccg ggcatctcgg
    45541 ttaattcgat tggagaacat ttcatgacag ctttcaccga tgttcaggtg gacttgcgtc
    45601 tgggacctga cgggcgaatc gacgtggaca tcgcctgcgt ttcctgtggg tacaacttgc
    45661 gcgggctcgc cccggacggt cgctgcccgg agtgcggcac ggccatcggc cgctcgacgc
    45721 gaggtgacta cctgcgattc tgtgatcctg gctgggtcga gaccttggct agcggcatga
    45781 actggatcgt ggcaggtctc attctcgcat tcgtgctcgg cctggttgcc ggcggtgcgg
    45841 taggactcac gaatagaccg gcctatgcta tgattggcat cgtcggcagt ctcgtggccc
    45901 tgatcggcta ttggagggta acaaccccgg acccccgcga accagaaccg caaggcgtcg
    45961 atgttcgcgc tattattcgc tatggttcag tgttgaatat cgttcttgcc gtggcttccc
    46021 agattgtcac acaaatgtcc ctctactgga agctgctcgc gtttccttcc gcggtggttg
    46081 gtctcatcgt cacagtgtcc attttcttat acgcgaaacg aatggcggtg cggattcctg
    46141 acgacaacct tgcgtctcag accggaaccg tcatgtgggg ttttgtcatc atgtctgttc
    46201 tgggtatgtt gttcctggcg gcgacgatgg ctttcgggcc ggcctttgcg acgatgaccg
    46261 tgcccgctgc cggtacgggg ggagcaaccg gtcccggcac cggtggaacc gttgtagcgt
    46321 ttcccgtcgg gttgatactg tttgcctcgc tggggtgtgt tatcacagtc gtgtcgctgg
    46381 tgttcggcat ctgggcattg gtgcttatca ttcgctaccg ccgggccttc atccaagccg
    46441 cgcgcgatgc ccgtgccaca tgggctgtcg atccggccgg tcagcaggcg atgggctgac
    46501 acggcgtcac gacgagattt gagtggatct acccctgcgg caacgggcaa cggccggcca
    46561 ggtggtttgc ccattcgacg caggcccccg tgccagatga ccatcgcacg tgaccaggtc
    46621 ccagagatcg cctggcccac attgcagcgg caattcgctc gccgctttca ttgacagaac
    46681 cgtgaaaaac ccattatgct gccactgctt attcattgac ggtttgccgc cctttgggcg
    46741 cgatgttgga aactcaaatg ggaaaaacga ctgttgtggc acgggcgggc aagtgcgatt
    46801 tgggcaaact ggttgccccc ttcgccaagc tcgcctgcgt ggatcgagtg ttggctctgg
    46861 tacctccggg catccagacg gccggcggga ttgaggcaat cgtgaccgat cagccggaca
    46921 gcggctctgc cctccggtcg gccatcgaga agctgaccgg cgactacgtc ctcttcgtcg
    46981 acggtgacaa cgagatccgt ctcgggcaat atgccctcga gcggatgatg gacgtggccg
    47041 agcagaccgg tgccggcatc gtgtatgccg actacgtcgt tgacgaagaa gcgggcgtcg
    47101 ccgatcgtcc taccattgat tatcagttcg gcagcatccg cgacgacttc gcgtttggcc
    47161 cgcttcgact ggtgtcccgc aaggctatcg aggatgccgc aaagcgccac ggtccgcccg
    47221 ccgatgtccg ttggtccgcg caatacgact tgcggctcaa gctgtccatc gatcacgcgt
    47281 tgctgcggct tcctgagtct ctctacaccg tcgtcgagga ccacagccgc aaccaacacg
    47341 acgagcattt tgcctacgtc gacccccgca acgtcgacgt gcagttggag cgcgagcagg
    47401 ttgccaccgg acatctcaag cggatcggcg cgtacctcga accgcgattc gagcctgtgc
    47461 cgccgccgtc ggagcggttt gaagttgaag ccagcgtggt cattccggtt cgcaatcgtg
    47521 aaaagaccat tgccgatgcc gtcaagagcg cgctctcgca agcggcttct tttccgttca
    47581 acgtgatcgt cgtggataat cactcaaccg atcgaaccac tgaaatcctc cgcgatctgg
    47641 cggcgggtga ttcccgcgtg gtgcatatcc tcccgactcg gacggatctg ggtatcggcg
    47701 gctgctggaa cgaggctgtt gcctcccccc ggtgcgggcg ctacgtcgta caactggatt
    47761 ccgatgacct ctacaacggc cacaatgccc tcgcgatcat ggtgaccgaa ctcaagaagg
    47821 gaccctacgc aatggttgtc ggttcgtacc gcatcgtgga cttcgatctg aacgaactgc
    47881 ctccggggtt gatcgatcac cgggagtgga cgcgggacaa tggccgtaac aacgtgcttc
    47941 gcgtcaacgg ttttggggcc ccacgggcgt tcgacgcgag cctgctgcgc cgccatccgc
    48001 tgccgaacgt cagctacggc gaggactacg cggtcggcct gcgtttctgc cgccgctatg
    48061 aggtaggacg cgtctgggag cccgtctacc tgtgccgccg ctggtcgggc aacaccgatg
    48121 ccgccctccc catcctcaag gccaatcagt acaacactta caaggaccgt ctgcggacaa
    48181 tcgagattct cgcccgtcag gagatgaacc ggcgtgaaaa aacgacctta tgagagacgc
    48241 cgattgcgag gcatcgtgaa tactgcgtgc tggttgacct tcggatgcat tgtcgtcggc
    48301 ttattcgttg cgttgtcttc gtctgcgcgg gccgcgtccg atggaggaga gcccggccga
    48361 ccatcaagta tgaatgccat gcaggtcgcg tgcgtgcagt ttgagaacac ggcggacatt
    48421 gccgagaacg cccgccgaat gatcgccatc ctggagaccg agtcggccgc cggcacgcgt
    48481 ctcgtcgttt ttccggaatg ctcgttgagc acctacggct cgcagatcgt tcgcgcgctg
    48541 acggccgagc agatcaatgc cgccctcaag accgtacagg aggcttgcgg ccggatcggc
    48601 atctacgcgg ttgtcggctc cgcatacatc gaaaacggaa aacgctacaa cggtgcgttc
    48661 gtgttcggac ccgacggtcg gctggtcaag cgatacgcca agatgcacgt ggtcaagccg
    48721 gaactctttg aggagggtga cgcgctggct gtctttcggg tcgacgatgt gccctgcaca
    48781 atcatgatct gccacgacga gcgatatccg gagatattcc ggattcccgt cctggcggga
    48841 gcgaagatcg gcatctacat cagttgcgaa tccaagacgc gggagaaatg ggacaactat
    48901 cgctgccagg tcattggccg ggcggtggag aaccagatca gcgttatcca ctgcaactcc
    48961 ggcgacggcg gcgctgacga gggttctcac ggacacagcc ggatcatcgc tccaagcggc
    49021 aacgtgttgg ccgaggcttc gactgaggtg ggccaggtta tccgggcgac cattcacccg
    49081 aaggaaagct cttccggtca tgcggagcgc ggagcggcca aaccgtcgct gcacgccttc
    49141 tggcaggaag gcctgcgggt gttgcgccgg cagaatcccg agttcttcga ggctgcgcct
    49201 gctcaacctg cggctgaccc gtccgcgcgg agtgaataag agagaggttc gcgacacctc
    49261 cggcggctcg ttcagaacct caatcgccgg cgaggggtct gtctacccat caacgcagaa
    49321 gactctgccc ttcgagaatc gcctcggcca acctggccct gaactcgatg acctcctgcg
    49381 gatctcgcgt gtagttggtc gtcgttgtca ctacgctgtc cggcaccctc agcaacgcag
    49441 cggcgcggtc cagaaaggcc cgatgggcgg gggccggcgg gcgcttacca atgcgctcaa
    49501 gggtctcccg cagcagccag agatactcgt aatcctcgaa accgtcgcgg atgttctcca
    49561 gccggatggt cgaaagcggc ccgtcgggtc cgggacataa aaggctgccg tcaccgttgg
    49621 cggtatactt cttctcttcg ttgactaggc ttccgggatt ccagtcggta taaggcccct
    49681 tggagatcgg acggctgttc ccttcccaca ggttgatcag gtagtaaagg aagccgccga
    49741 cctcatactt gtagctcatc accccggtca gcaggcgggc ctcgatcgcc ggatattcga
    49801 tgaaccagtt cgcgtacgga tgggtcggaa ccacgcagat gtaccaccac atctcgcggc
    49861 cttcacgccg cagctcacgt gccgcctcga tgtcatagct gtcggtgagc ggcacccaga
    49921 tatccacgac ctcacgcagg ccgcttcgct tgccgaaaga ccggtcaacg gccgtggtca
    49981 tcgttttcag ccccggaaaa cgcttgcgga tctcgccgaa cacctcgcgt atttcggtaa
    50041 acttctccct cttggcttcg tcgaagccgt aaacgtatgc cctgtcgaac accccggcgt
    50101 ttttcaactc ctgcacgtac tgcgagagct tcgggtcgtc tttgtccccc ttgccctgcc
    50161 ggaaaacacc gccgacgttg aaggcgttca ttccccgccc ggctccatac ttgagcaaac
    50221 cgatttcagg cgattcttgc cgatagagat gatcgatgtt gagccggtga tcgaggatga
    50281 agtcatagta gcgatacctc atctcgtcgt tccatctgcc tttgtagaag ttgccggtgg
    50341 ggccttcgtt gtaggtgaat gcattgcgca gatgggtgcc gttgggcagg gcaaaatccc
    50401 agacctgaac cttaagcctg agcccgatgg gcgaggcgtc acgcgccgtg atcgtcagcg
    50461 ttccctcgta gctgccggct tgcgtctcca cgcgtgtggc cacgtcgatc cagaacgcca
    50521 cgtgttcgcc ttccttcaca tcgctttttt gcagaaagct cagcagcggg tccggccacc
    50581 agcctttgtg ctcggcgtca tacggcgggt tgtcggcgac atcaacgtgt ccgacgagcg
    50641 agacttctat cctgccgttg aaggcttgtc ccattgccga cttgaggggg ccgacgctca
    50701 ccgtcacatc cgccaggtcg cggccgaacg gaatgaccac cacctggaac ccctcgtgct
    50761 cgtttcttgc cagcgctagc tcgtaatgat cgtcgaacac cccctcgaag gggtggtctt
    50821 tgatcatcac cttacgcatg gagctttcta ggccgaccgc gaactcggct tccggcatct
    50881 tcgcgagcat gcgggcatgg caggtcaccc cgtcaagctg catggtcaga tcgccgaccg
    50941 tggaagccag ccgttgccag tcctcagtag aacaggcttc cggctcggag gccggcttcg
    51001 tggcttcctc gaccagcggc cgtgccttgg ccaggatcgg ctcggctttg gcccgagctt
    51061 cggcccggaa cttcgccagc atgcgtgtcg cctcggcaaa tcgggccgcg atggtcctgg
    51121 ctcgctcaga aacctgcggc gagtgcatcg gatagaagcc gcggagcaac tccgtgccgg
    51181 cgtcgtcggc cacagccagg ctgacggagc gcacgcgatc ctcaatcaga acaaactcga
    51241 a
//
LOCUS       DAOTPW010000004        50170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_762_length_50170_cov_5.584017, whole genome shotgun sequence.
ACCESSION   DAOTPW010000004 DAOTPW010000000
VERSION     DAOTPW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 50170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50170
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_762_length_50170_cov_5.584017"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2552
                     /gene="mfd"
                     /locus_tag="P5316_00625"
     CDS             <1..2552
                     /gene="mfd"
                     /locus_tag="P5316_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRR83484.1"
                     /translation="EGSATSFLSYLPPNTIVAFDEPLEIQEIGKTFWNRLNGPAGMMP
                     VHRVFQRAGGFLQLHLHRLACSDAENRFSFHVQSLARFETKPTEALGELARIAAERDV
                     LLYCDNNSERERFTELWRSTIGDLPPRLSLPVGLMHHGFDWPDGGLTVVGHHEVFHRY
                     TQRRRIHRWHAARPVESWLDLNAGDYVVHVVHGIARFQGMRTMRKGDSEKQEEYLTLE
                     FADSARLHVPASQIDLVQKYVGIGTTPPPLSKLGGTRWAKTKERVEESVGDLAADLLR
                     IQAARASRPGIAYPGDTVWQREFEGSFIYTETEDQLSSLADIKKDMCRPQPMDRLLCG
                     DVGYGKTELAVRAAFKAVEYGKQVAVLVPTTVLAEQHERTFRERLADYPFVIESLSRF
                     KTRKAQSEIIDRTRKGQIDILIGTHRLLSKDVGFKDLGLLIIDEEQRFGVEHKERLKR
                     FRETMDVLTMTATPIPRTLHMSLLGIRDISSLATPPMDRRSIVTQVVNWSDQLIRDAV
                     LRELNREGQVFFVHNFVHDIRSFAERVSRIVPEARVIVGHGQMPERELERVMLQFVRH
                     QADVLVCTTIIESGLDIPNCNTILIDRADRFGLSELHQLRGRVGRYKHRAYCYLLLSP
                     DRTITPNAARRLKAIEEYSDLGAGFRIAMRDLEIRGAGNILGPEQSGHIAAVGYELYC
                     QLLDMAVRRLRNEPADVFRPVHLELDVPAFIPRNYIESDRQRMEIYRRVVRCRSLADL
                     GQLDKDIRDAFGPYPQAVADLLALAEIRILAQPFRIKAINRQPPDLIFTVEELALVEP
                     IFADGPGSARMADAHTVHLRLAPAYFETATLLAVLRRLLQPQTAVSTMPHR"
     gene            2549..3790
                     /locus_tag="P5316_00630"
     CDS             2549..3790
                     /locus_tag="P5316_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRR83485.1"
                     /translation="MRNARRINRIATASGLFLTIVCVTSCGDKETGPKREDHKAAHTP
                     RYHPDITREAAATGTATQQAAEVIVPSELPTGYTGTPVLFVNSQPITVSDVLEPLMPE
                     ILKMAGAATSEREYYRGISRLIADQIRYQSSTIVVYEQAKKKLNETVEQLLDKEAEKA
                     IQRTINTNYGGVRARYEAQLKALGLEMKDVKERIKRQLLVSSFLEERFSPMVAVPPRR
                     DLLKYYQAHLDAFTTPAKAELLMIEIPLRELLRKPVSQASESEIAEAREAARRELRRA
                     AEELESGVDFAAVARRYSKGAQRAKGGSWGEITPGSLTGSWAKAAEILFTLEEGRFSE
                     IIETEESSFIVKCGRKTPQERLTFEEAQARIIDQIREQEYSRMSSRYMGQLLDKAGID
                     PQQQQEFFIAVVSAAPRPNVK"
     gene            4098..5519
                     /locus_tag="P5316_00635"
     CDS             4098..5519
                     /locus_tag="P5316_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR83486.1"
                     /translation="MHDLTRIDRREFLKGTAAGIGSAAIAASSLKATEEEKIGASAPA
                     LKVDPKELIWRSKAPSMDYRRLGRTNYMVSRVVAGNAGNETLWRRMMAAGMNYFDTAS
                     NYGGGNHEIELSGTFVKYRDKLWITSKASDIAGYAKIDEDVRKLYAKAISEFLDDEKG
                     IGPGKELLDVHKKAIEKEKKTGRKPDLRPVGERIAKMYLDELDKSLAKMKVESVDCYM
                     MHGIEIPWIFDCLELWEAYERVHKAGKVKHFGYSVHRHQKDVLAASVEANKRGPWRID
                     LIMPAIDPGSFDNLRPELEALKKQDVGIIAMKTKGIKARPADAREEKFKALMGGKEYN
                     DDQRAKLWMLHLTDDLIDAVIARMGSMEDMEAMLPLAQVKLSAEAERELRAIVKLQMA
                     TACHLCGACDLNCPERIAVTNMIRYYAYLNDYKDRELAESLYRRLGYDPAAVCTNCGV
                     CEEVCPSHLPISELLHEVTAALA"
     gene            complement(5605..7179)
                     /locus_tag="P5316_00640"
     CDS             complement(5605..7179)
                     /locus_tag="P5316_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83487.1"
                     /translation="MSERETAGRHGARPASHFGRRNLLKGIAGGVIGSVAGTVQQHEA
                     AASAASVSKPANPHAAPRLIWKLDRPAKPALPYSYFWTWDHSTNWMLDDPGMLNFGCD
                     NRYLKRPETYLEDYRRLTDLAVGLGVRGILIWGFLRDDHGGVEFGKRVADYAAGKGII
                     IKPGVGTNWYGGVYYQGEHPYNIETFVRRHPDARLIDEQGKPQEHGVCPTHPRFVEWL
                     QEGVRWLFREFNVGGANLENGDYVVCYCPRCREHLAQWPKDEPPFWRDQYLGYNAALH
                     AIEGQLKDKFVTWATYQGFVPGKPADDRHRAAYLQCDRPALVDKLPPDAVCQWTLTSM
                     VRRQPLPLTAYLDNGTPAEAFSADLWPAGVKPPTVRSVGFLHQGSQWHGRTRYDQVVS
                     TIKEGCLRAYRAGLEGVSIHGEVSTMHIPWALNYLAFSHFIHWPEDSLRAWGRKTMGQ
                     VLASEAEGEAYAELFAHWQAGSLTDGLKKDLRQRTRALADDVAARGEHLHRFRFWNWL
                     HQVAEGTQDCHAASFL"
     gene            complement(7495..8529)
                     /locus_tag="P5316_00645"
     CDS             complement(7495..8529)
                     /locus_tag="P5316_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83488.1"
                     /translation="MSQQIPVAVVGAGHMGRHHVRIYHELPDCELVGIVDANPGRAAE
                     MASKHNTKAYPSIEPLLDKVRAVSVAVPTTAHVEVARPFLENRVAVLIEKPIAPDAAS
                     ARDLQQVARRHNTLLSVGHTERFNPVVRALGRMQVRPRFIETHRISPFTFRSADISVV
                     MDMMIHDIDIVLHLVKARPVRVDAVGVRVLSEHEDIANARLIFQGGCVANLTASRLAL
                     KTERKIRVFADEAYLSLDYQKKTGIAIKKDANLDLLKMAREHGAEELSQLVGRDFGSM
                     VKVEPLEIDDLEPLRVELQSFLNSVRSGEPPAVSAEDGVAAVELAEQIIAAARSHQWE
                     GVDADFAARA"
     gene            complement(8526..9398)
                     /gene="lpxA"
                     /locus_tag="P5316_00650"
     CDS             complement(8526..9398)
                     /gene="lpxA"
                     /locus_tag="P5316_00650"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRR83489.1"
                     /translation="MIHPSATVDSKAMLGRNVTIGPNCVVGPDVVLGDECTLRNNVTI
                     IGHTVCGRRNEFFPGSVIGAPPQDLKYKGGPTRVEIGDDNVFRELVTVHAGTEVAGGV
                     TWIGSHNRFLVGVHIAHDAKIGNDCILSNYVQLAGHVCLEDKVTMGGIIGVHHFTTIG
                     TLAYIGGMTRVINDVPPYMIVEGNPARVRGFNEQGMKRWGCTENQIRAVREAYRVLFG
                     PKAQSFGASMLDRLNVIEERQELNGEVRYLCESIRRSIKDGVYGRWLERDRRDTHVDR
                     QAFYGRPRSTEGIK"
     gene            complement(9502..10815)
                     /gene="lpxC"
                     /locus_tag="P5316_00655"
     CDS             complement(9502..10815)
                     /gene="lpxC"
                     /locus_tag="P5316_00655"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRR83490.1"
                     /translation="MKPQQTIRSPVELTGRGLFTGEQAVVRFYPAEPDTGVVFLRADQ
                     NPPIRVPALVENVAKRLRRTSIRNGTVQIETIEHCMSALAGLGIDNVLVELNANEVPS
                     FDGSCAPFVEKLKEVGIVAQDRPRQVFRIPEIVRVAEGGHYVMAAPAAEDEDTLEIIY
                     DLNYGSGPIGQQIFKIRVTPEEFERHLAAARTFVLEQEAEQFRASGLGSHLTYDDILV
                     FGKEGPIQNTLRFPDECVRHKILDLLGDLFLFGRFVSGYVFARESGHALNHELVRKLR
                     ELEQAAETKRKLKAEPVFDIRKIQRLLPHRYPFLMVDRVVEMEPGKHAIGIKNVTANE
                     EFFTGHYPGQPIMPGVLIIEAMAQLGGLLLAQELEHTGKVAVLLSLEGVKFRRPVVPG
                     DQLLLEASARRVKSRTGHVFCRAMVGDQLAAEADIKFMMVDADPL"
     gene            complement(10781..11833)
                     /gene="lpxD"
                     /locus_tag="P5316_00660"
     CDS             complement(10781..11833)
                     /gene="lpxD"
                     /locus_tag="P5316_00660"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRR83491.1"
                     /translation="MSKSYPLAEIAKWVGGVVRGDAGTRITGVAGLEEACSSDIAWLA
                     EDRYAPQLKNSRAGAVIVPMHFGATPMPAILCDNPSLAISAVLEKFAPPIPRPAPGVH
                     PTAVVSPSAKLGRDVAVGPLVVIGDNTVIGDRTVLHPHVFVGPDVRIGSDCEFWPGVV
                     IRERCAIGSRVTIHPNCSIGADGFGYQYVGGRHVKIPQIGSVEIEDDVEIGANSTIDR
                     AKFGVTRIGRGTKIDNLVQVAHNVRIGPNCMIVAQCGIAGSTRLGQGVVLGGKVGLRD
                     HISLGDGAQVAACACVSKDVPPGKTVIGSPAVDHEQFIRERAKVRRLPEMADQLRALI
                     KRVEQLEASADHSEPR"
     gene            complement(12089..12724)
                     /locus_tag="P5316_00665"
     CDS             complement(12089..12724)
                     /locus_tag="P5316_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRR83492.1"
                     /translation="MKGGKNTLLVMAAIAVVSIAAATMSGAQQRSGASGGTRVAVVDL
                     VKVFNEFDQTKAVNEAMRVYRDKLAQESDQKMSEIKAEQDILDKTDVTSPDRQARITK
                     IKRMQLEYNVWESLEKDFVSDQYVAWVKRTYEMVTDGIAEVAKKQGVQLVVTQEQVDT
                     TVTKPEPLLQQILNRKVVYADNSVDISADVLATLNANFAKLGGPASVHFGK"
     gene            13206..14432
                     /locus_tag="P5316_00670"
     CDS             13206..14432
                     /locus_tag="P5316_00670"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRR83493.1"
                     /translation="MSIIVQKFGGSSVADAEKIHRAARRAIKAKLGGHQVVMVVSAMG
                     KTTDKLIALAKEVNPNPPKREMDMLLTTGEQVSISLMAMALEAAGHQAISFTGRQIGL
                     VTDAAHTKARIRSIDADRIYKELNAGKIVIVAGFQGVTEDGALTTLGRGGSDTTAVAL
                     AAVLKDSICEIYTDVDGVYTTDPRIVPQARKMNEINYDEMLELASLGAKVMHSRSIEF
                     GKKYNVPIHVRSSLTDTEGTMIVAQTKGMEDVVVRGVTLKKDLARVLLVGVPNKPGIA
                     SKIFARVAQQGIVVDDIIQNIVEHASEANIGFTVAGTDLKDAELVCKELARDLGIRSV
                     EMDSGLAKVSVVGVGMKTHTGVASKMFGALAKAQINIQNITTSEIVVSCIVNAEEGEK
                     AVQVLHAEFELDKAND"
     gene            complement(14530..14796)
                     /locus_tag="P5316_00675"
     CDS             complement(14530..14796)
                     /locus_tag="P5316_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRR83494.1"
                     /translation="MKPEIRKPTEDERKEAHTWPTWSKEAGEFPWQYDEKETCLILDG
                     NVTVTNETGEKFNFGAGDWVVFPQGMKCTWKVNRPVRKHYNFGE"
     gene            complement(14793..15797)
                     /locus_tag="P5316_00680"
     CDS             complement(14793..15797)
                     /locus_tag="P5316_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRR83495.1"
                     /translation="MNLVTGATGLLGSHIVEQLRLRKRPVRVLVRRGGNVEWLKTQGV
                     EVADGDLTDKAALAKACQGVKVVYHAAARVGDWGPWEDFVRITIDGTRNLADAASAAG
                     CERFLHISSISAYGHPDGEGVVLDETAPLGTNLHKWSYYSRAKVEAEHILWHMHKAGK
                     IRLTVIRPSWLYGPRDRVTIFRLARMLREGKARILGDGKNRLNVVYAGNVAECAILAC
                     DTEAAVGQAYNACNDGEMRQEQWMNMLAAALGAPPVTRHVPYGVAYNVAFLLECFGHL
                     FRTKKPPMITRYAVWLMGRRVYFSAEKARKQLGWKSTVSYEEGVKKTIAWLREQEEKR
                     "
     gene            16194..17273
                     /locus_tag="P5316_00685"
     CDS             16194..17273
                     /locus_tag="P5316_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83496.1"
                     /translation="MSGKMKAALVCGWALSIYCLSATGAPDTWDPRLTTLGICWFEAD
                     VAPGTWYWKLVSGVYQDETQSGGNHHIYYKCLNAAGQPIENQKCWAGWGNTGSAAPCP
                     TIPSPPDGTAWQLTKGAFDGYWANFGMSGGWCPFWPEGPHGGYGAWVDGPSDQVWGMG
                     LPCNRHVNYLYVWKWTQAVQPLPTIARAPSAIVRTVTEGQNLPNDSFTVWNSGGLTLN
                     YTITDDGSWMEAIPAAGSCTTETDTITICYTVGDLLPGSYSATITISDPNSTNKTETI
                     SVSLTVEGIVIPGDFDGDKDVDQTDFGHFQACVSGSGIRQPEEACQAGDLDKDNDVDS
                     ADVGLFMGCLTGAGVQGNPDCVGSI"
     gene            17798..18763
                     /locus_tag="P5316_00690"
     CDS             17798..18763
                     /locus_tag="P5316_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR83497.1"
                     /translation="MRSETNIKLRRLPFPGFTLIEVLVVVAIIALLVAILLPSLARAR
                     AHARYAACLSNVKQFGLAGQMFAGRNNGQLPRGGDPDGSDHWTLMVAREMGLIKRILD
                     KTTCNQLRVDQLEIFHDPDRTAKGSAPWLGYIGNAFNPDQILGWPQMEMGAGIRIDSY
                     RRPSEVIYIACAESENMLDPSKSANWSNNPHVVRRNWQSQLQNLTSPNPSVVDGAIGW
                     LAANGGGIDAMDAWAGCHLPQGRILNNDANGDEPNQKFRRVARKLHLNRFTNGVFLDG
                     HAQGAQHERRGDLENYKTWLKRFGVKAETFLKPGNDPAAVNEIPP"
     gene            complement(19031..20524)
                     /locus_tag="P5316_00695"
     CDS             complement(19031..20524)
                     /locus_tag="P5316_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HRR83498.1"
                     /translation="MSVEPRHARFACRTLPTCLLMTGLLAFCATARDARGQSTAPARP
                     AQDPLKSIGPDDGYRGIWYSNQATNDEYVYKYSGGLGTYCAKHSPFAIYSAKADKTFF
                     CYGGTVKDKRELLEMVSYYDHATGTVPRPTILMNKGTDDAHDNPVISMDDKGYIFVFC
                     SSHGTSRPSYIFKSDKPYATDSFTVIYKGNFSYTQPWYWPGEGFLFIHTLYKGGRASH
                     WSTSRDGITWTEPQLLAFYDQGHYQVSRPGKGVVGLGFNYHPKAFHGDPKKRGLNWRT
                     NLYFAETRDFGRTWTNAKGDRLDLPLKAPQNPAMVREYEAEDLLVYVKDIDYDAQRRP
                     VILFVTSRGYEPGPRNDPRTWNIARWTGSEWVFSRVTNSDNNYDTGSLYIEPDGVWRI
                     IGPTEPGPQRYNPGGEVAIWISKDQGQSWTKIRDVTRNSELNHTYVRRPVDPHPDFYA
                     FWADGHGREPSESRLYFCNQAGDRVFRLPWQMTGDTARPERVAYSNP"
     gene            complement(21048..23036)
                     /locus_tag="P5316_00700"
     CDS             complement(21048..23036)
                     /locus_tag="P5316_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HRR83499.1"
                     /translation="MNGAPDIPKDGDHLAGAAFELDRINAELAALFGREPIKLAGGAD
                     ESRRDPLVICGLLGGKDVGKSTLVNTLAQKEISIDRDEVGAGTSRPIVYVHHDMVPPA
                     RMRLGRVDGGLDLQVSTHSADGIRNIVLVDLPDFDSDFMRHEAIVKAVVPHLDRVIWV
                     ATPRKIADRAWVAFAQDVVKDDTNVYFVLNKCDELLADEEDWTRPSDAAGIERQAEMF
                     CNRQRQWALRMLAEAGHEMNPDRVFLLAVKFAESSAFLERVGVIWDDPGWHRYGGDRP
                     VVTAIGRFLENEVKRLRRNVLGPLGADEAAEVKERNLREQIRRNTQRIRQAYEIDHWL
                     EQLQRATAGEHRQAMLNDAFGADYCRGVATALWRTRRSDIELADEVMDVRAGRWPLLR
                     VAYWLSRWVIRRVGRTLAGPGTAAPQTAVPAEELFRFRSRGLADRLEALGERLRTEHS
                     PIVRQFQLRERFPDTAELAARVEQELAELPLKDDESIINRLTIGYRPSWAKRLFVWCV
                     LFWFPFVQPVAEGLLEMAAAGTRIMGWAHGLYRLVWALGATQLLKGFVVVVGIYVACL
                     AAIYARSVQDVYRARGLLREGGRGRAEHLDARSGTEMLAEAIDRHLLEEVGGAMFRPF
                     EAAAIRLGQVAERLHRLDTPTPALHATTPGNEERRMQN"
     gene            complement(23033..24820)
                     /locus_tag="P5316_00705"
     CDS             complement(23033..24820)
                     /locus_tag="P5316_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83500.1"
                     /translation="MDLDAALNVVAQQGRCLRERLCLPWQEQAQQAVCALRFRRQAAA
                     AGQPVQLAAIVGGASSGKSTVFNNLLGGHRVSLVTIRSHATRGLIVAAHQKQYDRLDR
                     WLNHDRILLPTLESQPGRLDADLQGRPEAATVVQHDVAGFENTLLIDTADFTSSTAER
                     EGDVTLSLLPWFDRLVVIVDHERWFDRQVVDQLALLAGRFAQPRAVIFNRTSEEVLAE
                     PDRSRLEEQARHLGAEKVCILNYHRGRGFRRFDPQDLTEISRFLAEPPPRREPALHAE
                     IAREAAAALSANRTRLERLDQLERTLADVAARCAPATRWDCVTALMTHEERDRLGVVS
                     RILGISQMREWLGRQKRWLEEVMPRLPWTGKRDTAVGAAPPLSEKTDATREERGRDWL
                     NAQCERQIRRLNEEVTASDFWEDLRTVHGRPPLLVNRGLIESFRPRADETVARLAVAM
                     DEWNAKVQHECEGISPKLIGSLGMTILAGAAILIAVPGPVAALTPVIAAGALKAGLLK
                     LGAAGAFGAISARPLARLTEIIREKLLASTEFRRVREAAESMRRLIEEHGRAAADYLS
                     QQARAFTLPPNDPLGQALRILSRQEDRSA"
     gene            complement(24998..25792)
                     /locus_tag="P5316_00710"
     CDS             complement(24998..25792)
                     /locus_tag="P5316_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HRR83501.1"
                     /translation="MLKGYTWPRTPKGTSSVVPHPPWYFAGDCLAIEYEADAEAVRSF
                     LPAGLEFHSPQCAVYFIEWQYASETGEEYLDPARSLYHETIFLLSASHENSPCSYCPF
                     IWVDQDVSLVRGMIQGWPKQIGSTWITRPSPLVSKAAPMLGPGGRFGATLATKDRRLA
                     EALVTLREQTTQLPSPGFARAVNVRCFPELVAGRHDRPAVHELVQLKSRDVQVSLAWK
                     GDAVLKVFDHPYCELPALRPLKTLAGYRFSCALTVDDLIVLRDLRK"
     gene            26471..>27915
                     /locus_tag="P5316_00715"
     CDS             26471..>27915
                     /locus_tag="P5316_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83502.1"
                     /translation="MSRVYHSWAMLSVLVAMAFCVGRPAVAIDMYWTGEGDNNNWYSR
                     HFTGQYDSEHRPIYVNNWGQMGIDPPTPGASDHAIVGGDYTAEINYQNGPSIWELSVG
                     APATLWIGNAGSLTVNGPSIFNQGLIQIAYVGFAGGGTLSVTNGTVTGGGAIEFTGAG
                     TLSGALTLESGNLIHGSGGHLTATLLNQGLVSADVSNSWLSLETGAKTNEGVFSASGG
                     GRLDVRTSVYQTSLGQMVAGDSSTVQYYNGSSVTGGTTESSGTGKHFITGQNVTTTLT
                     DITNTGHWLAQDGSFVNLAGSTFTNNGTFEVGGYIGGAGMATLRVQADVTLAGTGDLF
                     MNPGIVDSVTGCTLTNSATHTIHGRGNLKAALDNQGQVIVDYNGGTLSLEGAAKSNEA
                     MIKATGGGILAVKTAITQSASGQVVVENNSRVQYYHGSSISGGTTASSGTGQHFITGQ
                     NVTTTLTDITNTGYWLAQDGSFVNLAGSTFT"
     assembly_gap    27916..28015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28016..29090
                     /locus_tag="P5316_00720"
     CDS             <28016..29090
                     /locus_tag="P5316_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83503.1"
                     /translation="NGTLEIGGYTGGSGRATLTPADGMILQGSGTLLLTPGHINSPAP
                     VTMTNAAGHTISGSGGRVYSNVTVNNLGTIEANGGTVELQTAPTQFSGTTLTGGTWKA
                     INGSLTVGGSPAVTTNQATVVLSGSSSAFAPVNSLADNQGSFSILGGRTFTTAGALTN
                     SGTIEIDVSSKLAVNGGYTQTAGLTSVDGTLSTTTGMVLSGGKLVGTGTINGATTVDA
                     GAALSPGHSAGVLTFQRLSLKSGATFDFEIDGPNADRIEIIGSGDVFTLAPGGVFTIN
                     LDVLNPTGALSEYTLFHWVGSTAIPSDTTWLVTGAPGVSGVVEIRPDLNSIMLTNVIV
                     PEPLSIGLFVVAAMGLPARRRSR"
     gene            complement(29468..30706)
                     /locus_tag="P5316_00725"
     CDS             complement(29468..30706)
                     /locus_tag="P5316_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRR83504.1"
                     /translation="MKCRVAGIVLAVLLYGSAVVAQPVDPPGADRQVGQAEKRARCRE
                     LGIQVGILPTGQWNAITDVPGVKVGHQTLRRGESIRTGVTVIVPHDGNTYREKVPAAV
                     YCGNAFGKLAGSTQIAELGTLETPIALTNTLGVAACMQGLIRYTLNQPGNETVGSVNA
                     VVGETYDGYLNDIRGMHVTPEDTLAAIAVAASGPVAEGSVGAGTGTSCFGFKGGIGTS
                     SRRLPHDLGGYTVGVLVQSNYGGVLTINGAPVGRELGDFSMSEYTTASGGDGSCMIVL
                     ATNAPLSPHGLERLAKRAVLGLGRTGSSMQNGSGDYVIAFSTAYRITDHDRVLDPPVA
                     TVAGRAMNPLFMAVVEATEEAVYNSMFQATSVTGIDGHHRRAIPLDRVVEICRRYNVL
                     NLQDRLPGVRDGRPATGESR"
     gene            complement(31562..32485)
                     /gene="ftsY"
                     /locus_tag="P5316_00730"
     CDS             complement(31562..32485)
                     /gene="ftsY"
                     /locus_tag="P5316_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRR83505.1"
                     /translation="MGLFDRLKQGLAKTRDKISRSFRSVLPFGRNIDDTIINQLEETM
                     LTDDMGPAVTARLIDEVRIAWKSGQIRETQEIIPFLKQKIVSYWPEPDRQLIRAASGP
                     TVILVTGVNGSGKTTSVAKLCNYLTREGNQVILAACDTFRAAAVAQLTEWSRRLGVQI
                     VKHQQGADPGAVAFDACEAALARNADVLIVDTAGRLHTQEHLMRELNKIQRVVEKKIP
                     GAPHEVLLVLDSTIGQNAINQAKVFSQHVKVTGLFLAKLDGSAKGGIVIGIRDQINIP
                     VKFVGLGETPDDIEPFNPESFVEALFAEPEA"
     gene            complement(32568..33017)
                     /gene="nusB"
                     /locus_tag="P5316_00735"
     CDS             complement(32568..33017)
                     /gene="nusB"
                     /locus_tag="P5316_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRR83506.1"
                     /translation="MKQSRHQARTAALQGLYQLDIQRAPADADAAECLKPILEEAGAS
                     PEVADYARQMTAGAWAKRDQYDRMIAEVTDHWEVSRMAVVDRNVLRMAIYELVERPEV
                     PARVVIDEAVEIAREFGDAETPQFVNGVLDAIWKKHPACRVARGEPV"
     gene            complement(33031..33522)
                     /gene="ribE"
                     /locus_tag="P5316_00740"
     CDS             complement(33031..33522)
                     /gene="ribE"
                     /locus_tag="P5316_00740"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRR83507.1"
                     /translation="MAENVKTITGELILRKGQKFAIVASRFNEFVCSRLLDGAIDALE
                     RHGCSMSDITVVRVPGAWELPLTARKIAATNQYAAVICLGCVIRGETPHFDYIAAEAA
                     KGIAQVGLSVEVPVVFGVLTADTLEQAINRAGAKSGNKGADAAMTAIEMANLYARLEG
                     KIT"
     gene            complement(33783..33989)
                     /locus_tag="P5316_00745"
     CDS             complement(33783..33989)
                     /locus_tag="P5316_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83508.1"
                     /translation="MDSVPQQADDLAVVEMKAPLPGKFHLLGPGICHYGLQGGYGRGR
                     LVPIHNPQLIRAEQAANVDNKGQS"
     gene            34002..34613
                     /locus_tag="P5316_00750"
     CDS             34002..34613
                     /locus_tag="P5316_00750"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRR83509.1"
                     /translation="MMEGGPSDFAHFIGTNYLNYHRADEFGWTAFSNPIGVSGILITG
                     DGWILYGRRNYRVACHPGYVQAFGGSLEMGERRADGTFDAFGCILRELEEEAGVQPED
                     VAETVCLGMARDRQIRQPELIFDVKIRQGREEMLDRLRHDDAEQEHAEIVACQDAPEA
                     IVPFIRRMVPMSPVAIGALFLHGWRHFGDRWYEQASQDLYQPQ"
     gene            34823..37381
                     /locus_tag="P5316_00755"
     CDS             34823..37381
                     /locus_tag="P5316_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRR83510.1"
                     /translation="MNKFQQRLQIIRGCLILLVLISCLFLWVSVLSFSPLDAPNPRVY
                     PHPAQAYNAGGKAGAWVAYKMFSYFGKGAYAAAAGLTMTIIVLAWKGCLPSLWQRIIG
                     VSLLISVTSAVSHMLLASPDRFREETGGILGYCLATWLQQSVNWMAWPMLFYGAVVGL
                     LFTAEELVLQIPAMLCRRRQQVTEMASAVAPAAQRITSITGPFRRAVARVKGLWPRPA
                     PAGAPTGGRSYAPVIVRPRPAVAKPKPAPSKSAPEDRSEQQSEGDDEPDEAEAKVEKT
                     ASAVAIEEKDNETAPPEAGDQDEPLLPAPVATKPPPMVVNFPTPPKPAAVPQTIVPPK
                     PYPAELGEWTFPPLSALIDPEYSFTSQQEALVREKAQDLQRALHEYGIEAHVVTIDTG
                     PVITMFELQVAPGVKLSQITSLANDIARALYAPSVRVVAPIPGRNTIGIEVPNLNKEK
                     VRLKELMTMGGDAVRRMALPLFLGKDASGRALIGDLAGMPHMLIAGTTGSGKSVCINS
                     IIMSILMTQRPDMVKLILVDPKMVEMSTFRDVPHLMCPIVTEIERAEKILSWATEKMD
                     ERYALLAEAGVRNIAAYNKLSEQEKLERFQPSTEEEKTQIPKHLPYVVIIVDELADMM
                     MMSAKEVEAHLARLAQKSRAVGIHIIVATQRPEAKIVTGLIKSNLPCRIAFRVASRMD
                     SRIVLDQNGAEVLMGQGDMLYLPPGSSKLVRAQGTFLEDSEVREIINFLRERSKPEFH
                     PELMQLGKAEASADGPRDELFDQAVEIILETGRGSVSLLQRRLAIGYSRASRLIEEMA
                     AAGIVGEYTGSQARKVLITAEEWQVRKEQEATRIRQAALQDDGVPDMLYDDDID"
     gene            complement(37566..39836)
                     /locus_tag="P5316_00760"
     CDS             complement(37566..39836)
                     /locus_tag="P5316_00760"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRR83511.1"
                     /translation="MIVRESESVVLSSRAFRIRLSTRPGTRRLVEEFCASINRDGRHD
                     IRISLLPWTGFAGVTSHTREFFITTEGPQSPTVDVLRDDDQVCQVRYGPLFAASWEIY
                     WTLGLANDDLRWDVSVHVGRKTLPDDEINLLAFDVPEDEEIADAWFDTGYILPPKHWF
                     AVPEPEVTYRGNEGGRGVLDRRVRLKFAGDGHEIMYLNFAGGYGLLVACAGDNLFRIV
                     PRATCRPAGPRDPRRLPPARFVFKSHERFCIGTPRWSENQVVEPQTRFQCSVVIQAHR
                     PKNVETMHLETPDRTVGKQTTRFHRTHAHGSIAHRAGFAGGWHNVGHPRAHGVSFEYY
                     LHGKAHFFGLHPAIDEVMARALDAVYQRETRSDGLVWGYGFDGRGAFHENNASMLIFL
                     ADYARRTGDLCRLKYGERWAEYILANCTDRPFLYLTPTSTGIPGPGNGARVCNWWDVV
                     SCGGYDAFINVLTYPALRDLAEMERAAGNVKLADHYAAAARRLRQSFNELFWDERAGR
                     YISWVDTQGGRHDYFFTSVNLIAAAESIADQPMRRRILDSIDRRVAELGYKGFSLPCN
                     LVSIPPEHYNAGDWWLETYGYPHFYDLFGTYENGGIFPWVSAYYIAAMAEFDPDKAYD
                     HYLAILNQYERDNLHGAGNGYFWDTETGLPTEGSKQEPYLANVAVTVWGFMSLFGLTF
                     DFTKGIAIRPRLPRRLNGSVFSIRYHGSKVTFHYRGYGVKVTSLRIDGRDLPPDGFIP
                     AERLREESIVEVEVSE"
     gene            39942..41183
                     /locus_tag="P5316_00765"
     CDS             39942..41183
                     /locus_tag="P5316_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRR83512.1"
                     /translation="MPELSQDNLREFVRRVASETPVLDIHTHVYSARFGGLLLYGLDE
                     LLTYHYLIAEVFRVAPLPYERFWGMSKSEQADYIWQHLFIEHSPVSEACRGVLTVLQA
                     FGIDPKQKNLTSIRKHLSAIPREAYIDQVFRLSNVRRVIMTNDPFDDKERPVWDQGPG
                     SDERFEAALRIDPLLNDWGFAVTRLAGWGYRVNGELGASDLTEVRRFLTDWVVRMRPR
                     YMAVSLPPDFTIPEDSPRAALIAGCVLPVCREAGIPFAMMIGVRRSLNPQLRLAGDGV
                     GAADVASVSRLCATCPDNRFLVTMLARENQHELCVAARKHPNLMVFGCWWFLNNPSLI
                     EEMTRMRLELLGTSVIPQHSDARVLDQLIYKWSHSRRIIADVLADKYIDLVRSGWMPS
                     EQEIRRDVADLFGGNFERFCG"
     gene            complement(41283..42770)
                     /locus_tag="P5316_00770"
     CDS             complement(41283..42770)
                     /locus_tag="P5316_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83513.1"
                     /translation="MCESCSSYVLNRRGFLAATGALGAAAFVSRAVARQVGAAAASAV
                     PKRDKKPARVLVAFLYPPADVVNEGKMEDSWRVHNWFTWPGNQFEPEQQERKFTAKIR
                     ETADKLGLVVEFMPKAIYQEAKVREFIEQAKAAKPDAVFIVNFWNTFSKWSHQMATEP
                     GAPTAIVYHPVGSNHQLPPDYLRNAEGLYYIHSIENWDEIERALTAVHARQMLAQSRL
                     LRISAKPAAEIISEPRLGVEVVGAPAEEFNAVFDGIADDDELVRDAMQFKKNAARVTD
                     VVDKYFVDAMRAHRAVEQMLHRYGADAITIECLQLKHRKPCISFATNNGNLVPCGCEN
                     DLNASLTLMLGRWLLGRGGFQHNPEFDTSENRYFASHCTCAWKLHGPQGPSQEYFVRP
                     FFHQLPKTAALDVQWTPGEPIILAKYLSGQDTLNCWTGKVIESPTSPPTGGCATRVLM
                     EMDNVDDVCKVYAGPHPILFCGNRGDAKRLKAFARMYRLTFQGNV"
     gene            complement(42990..44306)
                     /locus_tag="P5316_00775"
     CDS             complement(42990..44306)
                     /locus_tag="P5316_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRR83514.1"
                     /translation="MSRNALYNPACEALPRDELEQLQIERLQSTLNRVYRSVAFYRTA
                     FDANCVNLEKIRDLRAMRDLPFTTRDDLLRSYPYDMFAVPLRDIVRIHQGFAIAGTPV
                     VVGYTRNDLRNWTECAARVLTAGGITEHDVVQIALDYGLLPGAPGFQQGAELIGASVI
                     PASMTASMSRQIAIMKDFKTTALITTPSHALNIAAGLEEMGVHPERLQLRLALFGGER
                     WSDELQKRIEEKLHVIAIDTYGLTAIMGPGVAGECRQRCGLHINEDHFIVEVIDPTSL
                     EPLGPGCEGELVLTTITKEGFPLIRYRTGDITSVNSEPCACGRTLARMTRVSRRTDDL
                     IFFHGVGFFPVQIEKILYEVEGLSPHYQIILDRQGDVDTLEIKVEVLEDIPSLDEIKT
                     LEILRNQVARRIKTVLNLDAKVTFAEPKSLRSDSGAIPRVLDRRPG"
     gene            complement(44316..45053)
                     /locus_tag="P5316_00780"
     CDS             complement(44316..45053)
                     /locus_tag="P5316_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR83515.1"
                     /translation="MLRLTNVEAGYGALRVLKGISLHVSQGEIVAIIGANGAGKTTLL
                     KSIAGILQPRSGTILFDKQDLRGLPPDRIVQLGCSMVPEGRHVFPTMTVKENLILGAY
                     SCRGKGQTREAVKASMDEVCRLFDILKEREHQLAGTLSGGQQQMLAIARALMSKPRLL
                     MMDEPSLGVAPLVVRDIYRTITTLKRDGLTILLVEQNARAALAVADRGYVIETGQIVL
                     EGTSRELSDNREVQRAYLGKEYERIDE"
     gene            complement(45031..45816)
                     /locus_tag="P5316_00785"
     CDS             complement(45031..45816)
                     /locus_tag="P5316_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR83516.1"
                     /translation="MAEPLLTTHRLHRFFGGLHAVEDVSFSVPCGAIKAIIGPNGAGK
                     TTLFNLIAGTLSPHEGEVYFRGSRITGWRAHAIARLGIARTFQTTKLFPQMTVLENVM
                     VGRHPRTRSGFVAGMLNLPRTWREEKESRDKAMSILDDLGLSACASEIASNLSFGRQR
                     LVEFARALATEPQLLLLDEPAAGLNIYETQALADLILKIRGRGVTCLVVEHDMSLVMN
                     ISDDVVVLDQGHKIAEGPPRVIQRDPEVIRVYLGDDHAATDQC"
     gene            complement(45809..46879)
                     /locus_tag="P5316_00790"
     CDS             complement(45809..46879)
                     /locus_tag="P5316_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRR83517.1"
                     /translation="MRLGRYLPVAVTILLVVLIQTGLSAAEKPFYMTQITMSAYYALV
                     VIGLCLLMGYAGQASLGHAGFFAIGGYVSAVLTTCNLLAYQTHPLVRLCRWLGLTVEH
                     QDLYGRQMLSILPWPAFIAALLVTAVIALIIGMPVIRLKGHYLAMATLGFGLIVYRIV
                     LGAEVFGQADGISNVPPFKLIGDTLQVNGAPAFRVQNYYIAWVVVLAAMVLAINLVHS
                     RIGRALRSIHQSEEAAHSLGIDTYRYKLYTFVLSALFAAAGGALLTHYNASIGPSEVS
                     VMKSVRYVAIVAVGGMANLWGALLMGVVLNFLSLRGVFGTYDDAVFGVILIAMMIFAP
                     EGLLRVPRWRALAFLSTRRSHG"
     gene            complement(46879..47769)
                     /locus_tag="P5316_00795"
     CDS             complement(46879..47769)
                     /locus_tag="P5316_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRR83518.1"
                     /translation="MELTQALQYCVAGIRNGSVYAIVAIGFNIIYSTTGVINFAQGEF
                     LIVGAMTAISLNHFVPLPVAVALAVVITMLLGALVEFVFIRPVRHAPVLRLIVITIGI
                     SIVLREGMLHVWDEQVRALPYFTGTAVSTFPLFGARVSPQDLWVLGMCAVIVAGLTLF
                     FRFTPTGRAMRACADDRMAAQLCGIKDSRMVTLSFMLSAAIGALAGCAISPVMQTQYD
                     MGAPLAIKGFTVAILGGLGNSMAAVAAGLLLGLMEAFAVSQMPVAYKEAVALVVLLMV
                     LIVRPSGLFAGRQTIELRDH"
     gene            complement(47809..48999)
                     /locus_tag="P5316_00800"
     CDS             complement(47809..48999)
                     /locus_tag="P5316_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRR83519.1"
                     /translation="MTKRTCIILGIVGLGLFVGLAGCKDSSKAPTTTKPADAGPIRIG
                     AIFSITGPAAFLGAPEEKTTQMLVEKINNEGGVLGRKLEVIIKDSGASPEKALSLAKQ
                     LIDEEKVLAIIGPSTSGETMAIKQLCQDSKMILVSCAAAEKIVEPLASYVFKSPQKDS
                     DAVKVIYEAMKKRGISKIGVVVSSDGFGTAGREQLEKLAPEHGLTIAIAEVYPKEVTD
                     LTDVLTKVKGKDVQAVVNWSIVPAQSLVAKNMKQIGLDVPLFQSHGFGNIKYAQAGGE
                     AANGTLFPCGRLLVAEQLPDDHPQKALLMSYKKDYESKYNEEASTFGGHAYDALLILV
                     EAIKKAGTDDREKVRDAIENLKGLVGTAGIFNMSPTDHTGLDADAFELLTVKEGKFVI
                     APKE"
     gene            complement(49028..49459)
                     /locus_tag="P5316_00805"
     CDS             complement(49028..49459)
                     /locus_tag="P5316_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HRR83520.1"
                     /translation="MYAKQVCVFLENKKGRIAEVTGLLAGEGINIRALSLADTPDFGV
                     LRLIVDDRVRCVRVLKLNGFVVQETEVIAIEIDDRPGGLHRIVELLDREGINIEYIYA
                     FMKKKAEQALVVFKIDDAARAAETLRRSGIAILSEDAIKDL"
BASE COUNT         9511 a        15277 c        15684 g         9598 t
ORIGIN      
        1 cggagggaag cgctaccagc ttcctgtcct atctgccgcc gaacacgatt gtcgcgttcg
       61 acgagcctct ggagatccaa gagatcggca agacgttctg gaatcgcctt aacggcccgg
      121 cgggaatgat gccggtccac agagtgttcc agagagccgg cggatttctc caactacacc
      181 tccatcgcct ggcgtgtagc gacgccgaga accgcttcag ctttcacgtt cagagcctgg
      241 cccgattcga gaccaagccg acggaggccc tgggagaact ggccaggatt gccgctgagc
      301 gcgacgtcct gctctattgc gacaacaact ccgagcggga acggttcact gagttgtggc
      361 ggagcacaat cggcgatctg cccccgcggt tgtcgctacc tgtcgggctc atgcaccacg
      421 gattcgactg gcccgacggc ggcctgaccg tggtcgggca tcatgaggtc tttcaccgct
      481 acacgcagcg tcggcgaatc caccggtggc atgccgcccg tccggtggaa tcatggctcg
      541 acctgaacgc gggcgactac gtcgtacacg tcgtacacgg cattgcccga ttccagggca
      601 tgcgaacgat gcgcaagggg gactcggaaa aacaggagga atacctgacc ctcgagttcg
      661 ccgacagtgc gcgcctgcat gtcccggcaa gtcagatcga cctcgtccag aaatacgtcg
      721 gcatcggaac caccccaccg ccgctcagca agctcggcgg cacccgttgg gccaaaacca
      781 aggagcgagt cgaggaatct gtcggcgatc ttgcggccga cctcctgcgc attcaggctg
      841 cgcgggccag ccgcccgggc atcgcctatc ccggcgacac ggtctggcag cgggagttcg
      901 agggcagctt catctacacg gaaacggagg accagctctc cagccttgcc gacatcaaga
      961 aggacatgtg ccgtcctcag ccgatggacc gcctgctgtg cggcgacgtc ggctacggga
     1021 agaccgagct ggccgtccgg gcggcattca aggccgtcga atacggcaag caggtggccg
     1081 tcctggtgcc gaccaccgtg cttgccgaac aacacgaacg caccttccga gaacgactgg
     1141 cggattatcc cttcgtcatc gaatctctct cccgcttcaa gacgcggaaa gctcagagcg
     1201 aaatcatcga ccgtacgcgc aagggtcaga tcgacatcct catcggtacc catcgattgc
     1261 tgagcaagga cgtggggttc aaggacctcg gtctattgat cattgacgag gagcagcgat
     1321 tcggcgtcga gcacaaggaa aggctcaaac gcttccgcga gaccatggat gtgctgacca
     1381 tgacggcgac gcccatcccg cggacgctgc acatgtcgct gttgggtatc cgcgacatca
     1441 gctcgctagc gacacccccg atggatcgcc gcagcattgt cacccaggtg gtcaactggt
     1501 ctgaccaact gatccgcgac gccgtcctgc gggagctgaa ccgcgaaggc caggtcttct
     1561 tcgtgcacaa ctttgtgcac gacatccggt ctttcgccga gagggtcagt cgcatcgttc
     1621 ccgaggcccg cgtcattgtc ggtcacggcc agatgcccga acgcgagctc gaacgggtga
     1681 tgctccaatt cgtccgtcat caggccgacg tgctggtctg caccacgatc atcgagtccg
     1741 gactggacat tcccaactgc aacaccatcc tgatcgatcg ggccgaccga ttcgggttgt
     1801 cagagcttca ccagcttcga gggcgggtcg gtcgatacaa gcaccgcgct tactgttatc
     1861 tgctgctttc acccgaccgg accatcacgc cgaacgcggc ccgccgattg aaagccatcg
     1921 aggaatactc cgatctgggt gccggtttcc gaatcgcgat gcgcgatctg gagatccgcg
     1981 gcgcgggcaa tatcctgggg cccgaacaga gcggtcacat cgcagcggtc ggctatgagc
     2041 tctattgcca gttgctcgat atggcggtgc ggcgactcag gaacgaaccg gcggacgttt
     2101 tcaggccggt gcatttggaa ttggacgttc cggccttcat tccccgcaac tacatcgagt
     2161 ccgaccgaca acggatggag atctaccggc gcgtcgtgcg ctgtcgctca cttgcggatc
     2221 tggggcagct tgacaaggac atcagagacg ccttcggacc ctacccgcaa gcggtggccg
     2281 atctgctggc cctggccgaa attcggattc tggctcaacc gttcaggatc aaggcgatca
     2341 accggcagcc gccggacctg attttcaccg tcgaagagct ggccctggtc gagccgatct
     2401 tcgcggacgg tcccggttcg gcccggatgg ccgatgccca caccgttcac ctgcggttgg
     2461 cgcccgccta cttcgagacc gcgacgcttc tggccgtcct gaggcgtctg ttgcagcctc
     2521 aaaccgccgt atctacaatg ccgcatcgat gagaaacgcc agacggatca atcgaattgc
     2581 tacggcgtca ggcttgttct tgaccatcgt gtgtgtcacg agctgcggcg acaaggaaac
     2641 cggaccgaag cgagaggacc acaaggccgc ccacaccccc cgataccacc cggacatcac
     2701 cagagaggcg gccgcgaccg gaacggcgac gcagcaggct gccgaggtga tcgtgccgtc
     2761 cgagcttcca accggttaca cgggtacgcc ggtcctattt gtcaattccc agcccatcac
     2821 cgtctcggac gttctcgaac cgctcatgcc cgagatcctg aaaatggccg gcgccgccac
     2881 gtcggagcgc gagtactatc gcggcatttc gcggctgatt gccgaccaga tcagatatca
     2941 atcgagcacg atcgtggttt atgagcaggc caagaagaag ctcaacgaga ccgttgaaca
     3001 actgctcgac aaggaggccg agaaggccat ccagcgaacc atcaacacga actatggcgg
     3061 cgtccgtgcc cgatacgagg cccaactgaa agccctcggt ctcgaaatga aggacgtgaa
     3121 agaacgcatc aaacgccagc ttcttgtttc gagtttcctg gaagagcggt tctcgcccat
     3181 ggtcgccgtt cccccccgac gcgatctgct caagtattac caggctcatc ttgatgcctt
     3241 cacgacgccg gccaaggctg aattgctgat gatcgaaatc ccgcttcggg aacttctgcg
     3301 caaacccgtt tcgcaggcca gcgagagcga gatcgccgaa gcccgcgagg cggcccgccg
     3361 ggaactgcgt cgcgccgcgg aggagctcga aagcggagtg gactttgccg cggttgcgcg
     3421 gcgctattcc aagggtgctc agcgagccaa gggcggatcg tggggcgaaa tcacgcctgg
     3481 cagcctgacg ggttcgtggg ccaaggcggc cgagattctc ttcaccctcg aggaaggccg
     3541 attcagcgag atcattgaaa cggaggaatc gagtttcatc gtcaagtgcg gccgtaagac
     3601 gcctcaagag cggttgactt tcgaagaggc ccaggccagg atcatcgatc aaatccgaga
     3661 gcaggaatac agccgcatga gctcgcggta catggggcaa ctgctcgata aggccgggat
     3721 cgacccgcag cagcagcagg agttcttcat cgccgtggtg tcggccgccc cgcgccctaa
     3781 tgtcaagtag gtcgtcggca atgctcccgg catcaacggc tctttgagag tcgaggctgg
     3841 ggtacgatcg gcggcttgtc cgtcggcgtg tcggctggac ctgcacgatg ggatcagccg
     3901 gccgcgaggc ccgcagttca ccccgccgac tgtctcgcgg atcgggcaac agcatcggcc
     3961 gacggcggat tattgtcctt tgccggaaac aatcgctatg ctgttggcgc tctcaagatt
     4021 gcggtttgat gaccagggca agtctcgttg acagtgtccg tcaggggcga atttgcagct
     4081 tgataaggag tggacccatg catgacctca cgcgcatcga tcgtcgggag tttctgaaag
     4141 gtacggcggc aggcatcggc tctgcggcaa tcgcggcatc ctcgctcaag gcgaccgaag
     4201 aggaaaagat cggggcctcg gccccggcgc ttaaggtcga tcccaaggag ctgatctggc
     4261 ggagcaaggc gccgagcatg gactaccggc ggctcggccg aaccaactac atggtcagcc
     4321 gcgtggttgc gggcaacgcc ggaaacgaga ccctctggcg tcgcatgatg gccgcgggca
     4381 tgaattactt cgacacggca tccaactacg ggggaggcaa ccatgaaatc gagctgagcg
     4441 gcaccttcgt gaagtaccgc gacaagttgt ggattaccag caaggcctcc gacatcgccg
     4501 gttatgccaa gatcgacgaa gacgtgcgga agctgtacgc gaaggcgatt tcggaattcc
     4561 tcgacgacga gaaaggcatc ggccccggga aagaactgct tgacgttcac aagaaggcta
     4621 tcgagaaaga gaagaagacg ggcaggaagc ccgaccttcg tccggtcggc gagcgcatcg
     4681 ccaagatgta cctggacgaa ctggacaagt ccctggcaaa gatgaaggtg gagagtgtcg
     4741 actgttacat gatgcacggg atcgagattc cgtggatttt tgactgtttg gagttgtggg
     4801 aggcttacga acgggtccac aaagccggga aggtcaagca cttcggatac agtgttcacc
     4861 ggcaccagaa agacgtcctg gccgcgagcg tcgaggccaa caaacgaggc ccctggagga
     4921 tcgacctgat catgccggcc attgatccgg gcagcttcga taacctcagg cccgagctgg
     4981 aagcccttaa gaaacaggac gtgggcatca ttgcgatgaa gaccaagggc atcaaggccc
     5041 gcccggccga cgcccgcgag gagaaattca aggctctcat gggcggcaag gagtacaacg
     5101 acgatcagcg ggccaagctg tggatgctgc acctgacgga cgatctgatc gacgcggtta
     5161 tcgcacggat gggcagtatg gaggacatgg aagcgatgct gcctctggct caggtcaagc
     5221 tttccgccga ggcggagcgc gagctgcggg cgatcgtgaa gctccagatg gccaccgcct
     5281 gccatttgtg cggagcctgc gatctcaact gccccgagcg catcgcggtg accaacatga
     5341 tccgctatta cgcttatttg aacgactaca aggatcgcga actggcggaa agcctgtatc
     5401 gaagactggg ctacgacccg gcggccgtct gcaccaactg tggcgtgtgc gaagaggttt
     5461 gcccgtcgca cctgccgatc agcgagctgt tacacgaggt cacggcggca ctggcgtaga
     5521 ggccgggcga agcccgagtc ggacgttggc cggcggaggc acacgtcaac ggcgtccggc
     5581 cctctcaccg gatgcgcccg gtgtttacaa aaaactggct gcatgacagt cttgagttcc
     5641 ttcagccacc tgatgcaacc aattccagaa tcggaagcga tgcagatgct cgcctctggc
     5701 cgcaacgtcg tcggccaagg cgcgggttcg ttgtcgcagg tcttttttga ggccgtcggt
     5761 aagagaaccg gcctgccaat gggcgaagag ctcggcgtag gcctcgcctt ccgcttcgga
     5821 ggcgagaacc tggcccatcg tcttgcgacc ccaggcacgg agcgagtcct cgggccagtg
     5881 aatgaaatgc gaaaaggcga ggtagttcag agcccaaggg atgtgcatgg tcgaaacctc
     5941 gccgtgaatg ctgacgccct ccaggccggc tcggtacgcg cggagacaac cttccttgat
     6001 ggtgctgacg acctggtcgt atcgcgtacg accgtgccac tgactgccct ggtgcagaaa
     6061 cccgacgctg cggacggtgg gcggtttcac gccggcgggc cacaggtcgg ctgagaaggc
     6121 ttcggccggc gtgccattgt ccaggtaggc ggtcagcggc aaaggttgcc tgcggaccat
     6181 gctcgtcagc gtccattggc acacggcgtc gggcggaagc ttgtccacca aggccggccg
     6241 atcacactgg aggtacgcgg cacgatgccg gtcatcggcg ggttttccgg gaacgaagcc
     6301 ctggtacgtg gcccaggtga cgaatttgtc cttgagctga ccttcgatgg cgtgcaatgc
     6361 ggcgttgtag ccgaggtact gatcccgcca gaacggcggt tcatccttgg gccactgtgc
     6421 cagatgctcg cggcagcgcg ggcaatagca gaccacgtag tcgccgtttt ccaggttggc
     6481 gccgccgacg ttgaactcac gaaaaagcca tcggacgcct tcctggagcc actcgacgaa
     6541 acgagggtgc gtgggacaga cgccgtgctc ctggggcttg ccctgttcgt cgatcagacg
     6601 ggcatcggga tgtcttctga cgaaagtttc gatgttgtag gggtgttcac cttgatagta
     6661 gacgccgccg taccagttcg tgccgacgcc gggcttgata atgatgccct tgccggcggc
     6721 gtagtcggcc acgcgtttgc cgaactcaac gccgccgtgg tcgtctcgca ggaagcccca
     6781 aatcaggatg ccccggacgc ccaggccgac ggccaagtcg gtcaggcggc gatagtcttc
     6841 aaggtaggtt tccggtcgct tgaggtaccg gttgtcgcag ccgaagttga gcatgccggg
     6901 atcatcgagc atccagttgg tgctgtggtc ccaggtccag aagtaggagt acggaagggc
     6961 aggcttcgcc ggccggtcaa gtttccagat cagtcgcggg gcagcatgcg gatttgccgg
     7021 cttgctcacg ctcgctgctg aagccgccgc ctcgtgctgt tgaactgtac ctgccactga
     7081 tccgatgaca ccaccggcga tgcctttgag aagattacga cggccgaagt ggcttgcagg
     7141 ccgggcgccg tgacggccgg cagtctctcg ctccgacatt gtgtgctcct tatcaggcag
     7201 tcggcggacc tacccagaca acatctcgcc cgaccggagg acttccttca cgcaggactc
     7261 ccaaactatc ctgattgcca gcaaccgggc tccgcgtcgc cgaaccgcct tacctatctt
     7321 gccaatttgg ccatggttgg gttcgttcag caaagagacc gttgcggcac ttgccccgtt
     7381 gttttcggga ctgcctgagc cgtatcgaac agccgtgaca acagtcgtcc atcgcgttgg
     7441 ttcagaacat acgcggtgtt ccgaccgggc aggcgattac ggagcaagca actcctatgc
     7501 tcttgccgcg aagtcggcgt cgacgccttc ccactggtgt gatctggcgg cggcgatgat
     7561 ctgctcggcc agttccacgg cggcaacgcc gtcctcggca ctgaccgccg ggggctcgcc
     7621 cgatcggacc gagttcagaa acgattgcag ttcgacccgc agcggctcga ggtcgtcgat
     7681 ttccaacggt tcgactttga ccattgaccc gaaatcacgt ccgacgagct gcgagagctc
     7741 ctcggcgccg tgctcccggg ccatcttgag cagatcgagg ttggcgtcct tcttgatggc
     7801 gatgcccgtc ttcttctggt agtcgagtga gaggtaggct tcatcggcga acacgcggat
     7861 ctttcgttcg gtcttaagag ccaagcgcga cgcggtcaag ttggccacgc agccgccttg
     7921 gaagatgagg cgggcgttgg cgatgtcttc gtgctcggag agcacccgaa cgccgactgc
     7981 gtcgacgcgg accggccggg ctttgaccaa gtgcagcacg atgtcgatat cgtggatcat
     8041 catgtccatg acgacactga tatcggccga cctgaaagtg aaaggactga tgcggtgagt
     8101 ctcgatgaag cgcggcctga cctgcatgcg acccagcgcc cgaacgaccg gattgaaacg
     8161 ttccgtatgc cccacgctca gcaacgtgtt gtgcctgcgg gcaacctgct gcaagtcccg
     8221 ggcagaggct gcgtcggggg cgatgggctt ttcgatcagc acggccacgc ggttttccag
     8281 aaacggccgt gcaacctcga cgtgggcggt ggtcggcacg gcgacggaca cggccctgac
     8341 cttgtcgagc agcggctcaa tcgaagggta ggctttcgtg ttgtgcttcg aggccatctc
     8401 cgcagcccgg ccggggtttg cgtccacgat tccgacgagt tcgcagtcgg gcagctcgtg
     8461 gtagatgcga acgtggtgcc ggcccatgtg tcccgcgccg acgaccgcaa ccggaatctg
     8521 ttggctcact tgattccctc cgtactcctg ggtctcccgt agaaagcctg ccggtcgacg
     8581 tgcgtgtccc ggcgatcgcg ctccagccag cgtccgtaga cgccgtcctt gatgctgcgg
     8641 cgaatcgact cgcacaggta gcggacctcg ccgttcagct cctgccgttc ctcaatcacg
     8701 ttcagccggt cgagcattga ggccccaaag ctctgggcct tgggcccgaa gagcacccgg
     8761 tatgcctcgc gcaccgcccg gatctgattc tcggtgcatc cccagcgttt cataccctgt
     8821 tcgttgaagc ctcgcacacg tgccgggttt ccttcaacga tcatatacgg cggcacgtcg
     8881 ttaatgacgc gggtcatgcc gccgatgtaa gccagcgtgc cgatggtggt gaagtggtgg
     8941 acgccgatga tcccccccat cgtgaccttg tcttccaggc agacgtgccc ggcgagctgc
     9001 acgtagttgg acaagatgca gtcgttgccg atctttgcgt cgtgggcgat gtgcacaccc
     9061 acaagaaagc ggttatgcga accgatccag gtcacgccgc ccgcgacctc ggtgccggcg
     9121 tgtacggtga ccagctcacg aaagacgttg tcgtcgccga tttcgacgcg ggtcgggccg
     9181 cccttgtact tgaggtcttg cggtggggca ccgatcaccg agcccgggaa gaactcattc
     9241 cggcgaccgc acacggtgtg cccgatgatc gtcacgttat tgcgcaaggt acactcgtcg
     9301 cccaacacca cgtccggtcc cacaacgcaa ttcggcccga tggtgacgtt ccgaccgagc
     9361 atggccttag aatcaacagt tgccgacggg tgaatcattt gttccgctct tagctgccag
     9421 cttggggctg tcagtcctta gctctcggtt cctggctgcc aacctcttgt tttcccggcc
     9481 gacagcgaaa cagcttctcg ctcacaacgg gtcagcatcg accatcatga atttgatatc
     9541 ggcctcggcc gccaactggt ctcccaccat tgcccgacag aacacgtgac ccgtccggga
     9601 tttgacccgg cgagccgagg cctccaacag cagttgatcg ccgggaacga ccggccggcg
     9661 gaacttgact ccctccaggc tcagcaagac ggccaccttg ccggtgtgct caagctcctg
     9721 ggccagcaac aggccgccga gctgggccat agcctcgatg atcagcacgc ccggcatgat
     9781 cggctgaccc gggtagtgtc cggtgaagaa ctcctcattg gcggtgacgt tcttgatgcc
     9841 gatcgcatgc ttgccgggtt ccatctcaac cacccggtcg accatcagga aggggtagcg
     9901 atgcggcagt aagcgttgga tcttgcggat gtcgaaaacg ggttcggcct tcagtttccg
     9961 cttggtctca gccgcctgct ccaactcgcg cagcttgcgg accagttcgt gattcagggc
    10021 gtggcccgat tcacgggcaa atacgtaccc ggacacgaac cggccgaaca ggaacaagtc
    10081 gccaagcagg tcgaggatct tatgccggac gcactcatcc ggaaaacgaa gcgtgttctg
    10141 aatcgggcct tccttgccaa atacgaggat gtcatcgtag gtcaggtgcg agcccaggcc
    10201 cgacgcccga aactgctcgg cttcctgctc cagaacgaag gtgcgggctg cagcgagatg
    10261 ccgctcgaat tcctcggggg tgacacggat cttgaagatc tgctgcccga tcggcccgct
    10321 gccgtagttg aggtcgtaga tgatttcgag ggtatcctca tcttctgcgg ccggagcagc
    10381 catgacgtag tggcctcctt cggccacgcg gacgatctca ggaatgcgaa acacttgtcg
    10441 aggacggtcc tgtgcgacga tgccaacctc cttgagcttc tcgacgaagg gagcacagct
    10501 tccatcaaac gaaggcacct cgttggcgtt cagttccaca aggacgttgt cgatgcccaa
    10561 gcccgcgagc gccgacatgc aatgctcgat ggtctcaatc tggacggtcc cgttgcggat
    10621 agaggtccgg cgcaggcgtt tggcgacgtt ctcgacaaga gcgggaaccc ggatgggcgg
    10681 gttctggtcc gccctcagga ataccacgcc cgtatcgggc tcggccgggt agaagcggac
    10741 gaccgcctgc tcgccggtga acagccctcg cccggtcagc tcaacggggc tccgaatggt
    10801 ctgctgaggc ttcaagttgc tcgacccgct tgatcaaggc ccggagctgg tcggccatct
    10861 ccgggagccg tcgcactttg gctctctccc ggatgaactg ttcgtggtcg accgccgggc
    10921 tgccgatgac cgtcttgcct gggggcacat ccttggaaac gcacgcgcaa gcggccactt
    10981 gcgcgccgtc gccgagcgag atgtggtccc gcaagcccac ttttccgccc agcaccacgc
    11041 cctgtcccag gcgggtgctg ccggcgatgc cgcactgagc cacgatcatg cagttcggcc
    11101 caattcggac attatgggca acctggacaa ggttatcaat cttggtgccc cggccaatgc
    11161 gggtaacccc gaatttagcc ctgtcaatcg tgctgttggc ccctatttcg acgtcgtcct
    11221 cgatctcgac gctgccaatc tgggggatct tgacgtggcg gccccctaca tattggtagc
    11281 cgaagccgtc ggcgcctatg gagcagttgg ggtggatggt tacgcgactg ccgatcgcgc
    11341 accgctcccg gatcacgacc ccgggccaga actcgcaatc gctgccgatg cggacatccg
    11401 gtccgacgaa aacgtgagga tgaagcacgg tccgatcccc gatgacggtg ttgtcgccga
    11461 tgaccaccaa cggtccgacg gccacatccc ggccgagctt ggccgacggt gagaccaccg
    11521 ccgtgggatg aacgcccggc gccggccttg ggatcggcgg ggcaaacttc tcaagcactg
    11581 ctgagatcgc cagggaaggg ttatcgcaca gaatggcggg catcggggtc gccccgaagt
    11641 gcatgggtac gatcaccgcg ccggcccggc tgttcttcaa ctggggggcg tatctgtctt
    11701 cggccagcca cgcaatgtcg gacgaacaag cctcctcaag cccggccacg ccggtgatcc
    11761 gagtgccagc gtcgccgcgg accactccgc cgacccattt ggcgatctcc gccagaggat
    11821 acgacttgct catggatgca catgctcctc ggaggcttgc tgagcccaga atcctcgcgg
    11881 gctccggccc cccgacgccg gctcgagaat cgtttctgtg gccctcatcc tcaccggacg
    11941 cggagcgaat gccgcacagg tgagaaggcc ctgcctcacg gccggcgttc cgcaagactg
    12001 gcgcggaaag gattccgggc ccagcggtgg attccgggcc aagcggtgcc tctcagcctt
    12061 cggcagtccc tgcggttcaa gccgagccct acttgccgaa gtgcaccgac gccgggccgc
    12121 cgagcttggc gaagttggcg ttgagcgtgg ccagcacgtc agccgagata tccacgctgt
    12181 tgtccgcgta gaccaccttg cgattgagga tctgctgcaa caggggctca ggcttggtca
    12241 cggtggtgtc cacctgttcc tgcgtcacga ccagttgaac accctgcttc ttcgcgacct
    12301 cggcgatgcc gtcggtgacc atctcgtagg tccgcttgac ccaggccaca tactggtcgc
    12361 tgacaaaatc cttttccagc gactcccaga cgttgtattc cagttgcatg cgtttgatct
    12421 tggtgatgcg ggcctgccgg tcggggctgg tgacgtcggt cttgtccaag atgtcctgtt
    12481 cggccttgat ctcggacatt ttctggtcgg actcctgggc caacttgtcg cgatagaccc
    12541 tcatggcctc gttgaccgcc ttggtctgat cgaactcgtt gaagaccttg acgaggtcga
    12601 cgacggcgac ccgggtgccg ccgctcgcgc cggaccgctg ttgagcaccg ctcatcgtcg
    12661 ccgccgcgat cgaaaccacc gcgatcgccg ccatcaccaa cagagtattc ttgccgccct
    12721 tcatggaaga ctccttctcc ggccaggttc gccagttcgc tcatccctcg acacacggaa
    12781 cgccagcgcc agccaaggcc tcccggttcg aacgggaacc attagaccgg gcaatccggc
    12841 cggccgcaag tctgtggtct accggcgctt gcggcgggtc cgctacggcc cgggcgcatc
    12901 tttcaggatc cccaagcgtc agcgaggggt cacgccttgg ggcaaccaca gcgtcggcag
    12961 agaaaaccgg aagccgttga tggaacgaga ataaggcacc agaaagctgc gggcggttgt
    13021 ccggggcggg ggggtgttat tcgacacgat aatgcgatgt ataagtcaat ccgcaaacac
    13081 tgggggcttg ccttggggcc atcttgtgtt aagcttcgcg ggcttgcgcg ccgcgggttt
    13141 cggggggatg atcgatccgc ggccgggccg cggcggagac aaggccgttt ttcggtgcga
    13201 ggaacatgag cattatcgtt caaaagttcg gcgggagcag tgtcgcggac gcggaaaaga
    13261 tccaccgcgc cgcccggcgg gcgatcaagg cgaagctcgg cgggcaccag gtggtgatgg
    13321 tcgtttcggc catgggcaag acgaccgata agctcatcgc tttggccaag gaagtcaacc
    13381 caaacccgcc gaagcgcgag atggacatgt tgctgaccac gggcgagcag gtgtcgattt
    13441 cgctgatggc catggccttg gaggccgccg ggcaccaggc gatcagcttc accggccgac
    13501 agatcggcct cgtgacagac gccgctcaca ccaaagcccg gatacgctcg atcgacgccg
    13561 acagaatcta caaggaactg aacgcgggca agatcgtgat cgtggcgggt tttcagggag
    13621 tgaccgagga cggagccttg accaccctcg ggcgaggcgg ttcggacacc acggccgtgg
    13681 ccctggcggc cgttctcaag gactcgatct gcgagatcta caccgacgtc gacggcgtgt
    13741 acaccaccga cccccggatc gtgccccagg cgcgcaagat gaacgagatc aactacgatg
    13801 aaatgctcga gctcgcatcg ctgggcgcca aggtgatgca ttcgcggtcg atcgagttcg
    13861 gcaagaagta taacgtgcct atccacgttc gcagttcgct tactgacaca gaagggacca
    13921 tgatcgtggc tcaaaccaag ggaatggaag acgtcgttgt tcgcggtgtg acgctcaaga
    13981 aagacctggc ccgggttctt ctggtcggcg tgcccaacaa gccgggcatc gcctcgaaga
    14041 tttttgctcg cgtcgcccag cagggcatcg tggtggatga catcattcaa aacatcgtcg
    14101 agcacgcctc cgaagccaat atcggcttta ccgtggcggg gaccgacctc aaggacgccg
    14161 agctggtctg caaggaactg gcccgcgatc tggggattcg gtcggtcgaa atggactcgg
    14221 gcctcgccaa ggtcagcgtc gtcggcgtgg gcatgaagac tcacacgggc gtggcctcca
    14281 agatgttcgg cgctctggcg aaagctcaaa tcaacataca aaacatcacc accagcgaga
    14341 ttgtggtaag ctgcatcgtc aacgccgagg agggcgaaaa agccgttcag gtactccacg
    14401 cggagttcga gctggataag gcaaacgact gaagaaagga tgaaggcagg gcacggtcgg
    14461 agttgccgaa ggcaacggag acctgccgct cttcgaacag accgacatgc cgataaacaa
    14521 aagactcgct cactcgccga agttgtagtg ctttctgacc ggccggttga ccttccacgt
    14581 gcatttcatg ccttgcggaa agaccaccca atcccccgcg ccaaagttga acttctcgcc
    14641 ggtttcattg gtgacggtga cgttgccgtc gaggatcagg cacgtttcct tttcgtcgta
    14701 ctgccagggg aactcgccgg cctccttgct ccacgtcggc cacgtgtgag cttccttcct
    14761 ctcatcctca gtcggcttgc gaatctcagg tttcatcgtt tttcctcctg ctcgcgcagc
    14821 cacgcgatcg ttttcttgac gccttcctcg tacgagacag tcgatttcca gccgagctgc
    14881 ttgcgggcct tctcggcgct gaaatagacg cggcggccca tgagccacac ggcgtatcgc
    14941 gtgatcatcg ggggtttctt cgtgcggaag agatgcccga agcactcgag gagaaacgcc
    15001 acgttgtagg cgacgccata cgggacgtgc ctggtgaccg gcggggctcc gagggcggca
    15061 gccagcatgt tcatccactg ctcctgtcgc atttcgccgt cgttgcacgc gttgtaggcc
    15121 tggccgacgg cggcttcggt gtcgcacgcc aggatcgcac actcggccac gttgcccgcg
    15181 tagacgacgt tcagccggtt cttgccgtca ccgagaattc tcgccttgcc ctcgcggagc
    15241 attcgggcca agcggaaaat ggtgacgcgg tcgcgaggcc cgtacagcca actcggtcgg
    15301 atgacggtga gcctgatttt cccggccttg tgcatatgcc agaggatatg ctctgcctcg
    15361 accttggccc gtgagtagta gctccactta tgcaggttcg tgccgagcgg tgcggtttca
    15421 tcgaggacga cgccttcgcc gtcggggtgg ccgtaggcac tgatggagct gatgtgcagg
    15481 aagcgttcgc atccggcggc cgaggccgcg tcggccagat tgcgggtccc gtcgatggtg
    15541 atcctgacga aatcttccca cgggccccaa tcgcccacgc gtgcggccgc gtggtacacg
    15601 accttcacgc cctggcaggc cttggcgagg gccgccttgt cggtcagatc gccgtccgca
    15661 acctcgacgc cctgagtttt aagccattcc acgttgccac cgcggcgaac cagcacccgc
    15721 accgggcgtt tgcgcagccg gagctgctcg acaatgtgac tccccaacag tcccgttgca
    15781 ccggtgacca gattcatgac tgctcccgca tattgatgcc cggtgtttga caggccgtct
    15841 cagaacctgc gtaagaccgg ccatgagctc gtccaatccg aggtttccgg gaaagcctcg
    15901 gtggagagcg ggcaagatag acccctgccg caacagcgtc aagaacggcc gcttgtcatc
    15961 cggatgatcg aggggctcgc tgcgcctctt ccggcttgct ccgcaggtgc gatgtgaccc
    16021 gtgttgccaa cgcccgccgc tttccggttg aagtccctca cgtcatcggg caatcaattc
    16081 ccggcatcag atgtacttga tccgccttga agaatcggat aacatggaat catcgcggag
    16141 gcatctcccg ggcggctcgc cgggttcagg cgacatcgcg cgaggtacgt tccatgtctg
    16201 ggaaaatgaa ggcggctctc gtctgtgggt gggcgctcag catctattgt ctctcggcca
    16261 cgggagcccc cgacacctgg gacccgagac tgaccacgtt gggcatttgc tggttcgaag
    16321 ctgatgttgc ccctggcaca tggtactgga agctggtaag cggggtttat caggacgaga
    16381 cccaatcagg gggaaaccac cacatctact acaagtgtct caacgccgcg ggccaaccta
    16441 tcgagaacca gaaatgctgg gccggctggg gcaacaccgg gtctgccgcg ccgtgcccca
    16501 ccatcccgag tccgccggat gggactgcgt ggcagctaac caaaggtgcg tttgacggct
    16561 actgggccaa cttcggcatg tccggcggat ggtgcccgtt ctggccggag ggtccccacg
    16621 gaggttatgg cgcctgggtc gacggcccga gcgatcaggt ctggggcatg ggcctgccct
    16681 gcaatcggca cgtcaattac ctttacgtgt ggaagtggac gcaggccgtc cagccgctgc
    16741 cgaccatcgc gcgggcgccg agcgccatcg ttcgtacggt gaccgagggc caaaacctgc
    16801 cgaatgacag cttcacggtc tggaactccg gcggcctcac cctcaactac accatcaccg
    16861 acgatgggag ttggatggag gcgatcccgg ctgccggctc atgcaccact gaaaccgaca
    16921 ccatcacgat ctgctacaca gtcggtgatc ttctgcccgg ttcatactcg gcaacgatta
    16981 ccatctccga ccccaactcg acgaacaaga ccgagaccat ttccgtgagc ctgacggtcg
    17041 agggcatcgt gattcccggc gattttgacg gggacaagga cgtggaccag accgacttcg
    17101 gccattttca ggcctgcgtc agcgggtccg gaattcggca gcccgaagag gcttgccaag
    17161 cgggcgacct ggataaggac aacgatgtgg actcggcgga tgtggggttg ttcatgggct
    17221 gtctgaccgg cgccggcgtc caaggtaatc ctgactgtgt tgggagcatt tgatccgcct
    17281 tgaacagtgt tgttcctgtt ctgtgccacg ctgttgggtg ggccttattg cggtctcaag
    17341 cgaagtcctt gccggttgac ctgaagctcc ggctccggga ttgtggtatc ctcggtgagg
    17401 tccaggactt gcgatcaggt acagtctccg ttgtctggct gcgcctcgat cgcccggccc
    17461 agaagcggca tcggctgact gacctgactc atgcccgtct tcgacgcacg aaaccgtgtc
    17521 ttcgagacag tcgcccggac ggtgggtgat tcgcttcggc tgccggcgcg gcctttcggg
    17581 cgcgggcgtc gaaattctgg cagacggtag ggggcaatac tcctgagccg atgtcaggga
    17641 attaccgaaa aacatatgga cgttagcggg gcgttcccgt agaatcatat gcagccccgg
    17701 accgcatggc cgggtaagct catgcagcga cgtatgggac cggaggtgtt ctcattgctt
    17761 gccttgtggc gagagaggct ctaaggagac ccatccgatg cgatctgaga ccaatatcaa
    17821 gcttcgcagg ttgccgttcc cgggttttac gctaatcgag gttctggtcg ttgtggccat
    17881 cattgccctg ctggttgcga ttctgctccc atcgcttgcg cgggccaggg ctcacgcgag
    17941 atacgctgcc tgcctgagca acgtgaagca gtttggtctg gcaggccaga tgtttgcggg
    18001 ccggaacaat ggacagcttc cccgcggcgg cgaccccgat ggaagcgatc attggacgct
    18061 tatggtggcg cgggaaatgg gtcttatcaa gcggattctt gacaaaacca cctgcaatca
    18121 attgagggtc gatcagcttg agatctttca cgatcccgac cggacggcca aaggttcagc
    18181 cccgtggctc ggatacatcg gcaacgcctt caaccctgac cagattctcg gatggccaca
    18241 aatggagatg ggtgcaggaa tcagaatcga cagctacaga cgtccgtcag aggtcatcta
    18301 tattgcctgc gcggaatcgg agaacatgct ggacccgtcg aaatcggcaa actggagcaa
    18361 caacccgcac gtggttcgtc ggaattggca gagccagttg cagaacctga cgagcccgaa
    18421 cccgagcgtt gtcgacggcg cgattggctg gttggccgcc aatggcggcg ggatcgacgc
    18481 catggatgcc tgggcgggtt gtcatttgcc gcagggcaga atcttgaaca acgacgcgaa
    18541 cggcgacgag ccgaaccaaa agttccgtcg ggtggcgagg aaactgcacc tcaatcgctt
    18601 taccaacggc gtgtttctcg acgggcatgc gcagggcgcg cagcatgagc ggcggggcga
    18661 tctggagaac tacaagacct ggctcaagcg gttcggggtg aaggcggaga cattcctcaa
    18721 gcccggcaac gaccccgcgg ctgtcaatga gataccgccc taaggcggac cgtctttttg
    18781 cggaccgcaa cccgagcgca accgcgatcc acagaatgcg cgcaagccgc acagatcttt
    18841 ggccgcaagg ttctggtgct acaagcaaat tggtttggat gcagcgaacc gtcaaggggc
    18901 cttgcgggtc cggagggcgg cgtgtgggcg gtaccgatgt ctccggcatg aaggtttcgt
    18961 gcaagaggcg gaacggtctg cgccatgggc gccgccagtg agcgcaggca tttgctcttg
    19021 ttgccgtgtg tcacgggttt gagtacgcaa cccgctcggg tctggccgtg tcgccggtca
    19081 tttgccaagg cagtcggaac acgcgatcgc cggcctggtt gcagaagtag agccgtgact
    19141 cggatggctc gcggccgtgg ccatcagccc agaaggcgta gaaatccggg tgcgggtcaa
    19201 ccggccgtcg gacgtaggtg tggttcagct cgctgttgcg agtgacgtca cggatcttgg
    19261 tccacgactg cccctgatct ttgctgatcc agatggcgac ctcgccaccg ggattatatc
    19321 gctgtggccc aggctcggtc gggccgatga ttcgccagac cccgtcgggc tcgatataca
    19381 ggcttcccgt atcatagttg ttgtcggagt tggtcacgcg gctgaagacc cactcgctgc
    19441 cggtccaccg ggcgatgttc caggtccgcg ggtcgttgcg gggtccgggt tcgtaaccac
    19501 gactggtgac gaacaggatc accggcctgc gctgggcatc gtagtcgatg tccttcacat
    19561 agaccagcag atcctcagct tcgtactcgc ggaccatcgc cgggttctga ggtgccttca
    19621 gcggcagatc gagcctgtcg cctttggcgt tggtccaggt gcgcccgaaa tcacgggtct
    19681 cagcgaagta caggttcgtt cgccagttca gtccgcgttt cttgggatcg ccgtggaaag
    19741 ccttcggatg atagttgaag ccgaggccga ccacgccttt tcccggccgg ctgacctggt
    19801 agtggccctg gtcatagaat gccaacagtt gtggttcagt ccacgtgatg ccatcgcggc
    19861 tggtactcca gtgcgacgca cggccgccct tgtacagcgt gtgaatgaac aggaagccct
    19921 cgccgggcca gtaccagggc tgcgtatatg agaagttgcc cttgtagatc acggtgaatg
    19981 aatccgttgc gtagggcttg tcgctcttga agatgtacga cggccgcgat gtcccgtgtg
    20041 aactgcagaa cacgaagatg tagcctttgt cgtccatgga gatgaccggg ttatcgtgag
    20101 cgtcatcggt gcccttgttc atcaggatcg tcggtcgcgg gacggtgccg gtcgcgtggt
    20161 cgtagtatga gaccatttcg agcagttccc tcttatcctt gaccgttcca ccatagcaga
    20221 agaacgtctt gtcggccttg gccgagtaga tcgcaaacgg cgagtgtttg gcgcagtagg
    20281 tgcccagtcc gccggagtat ttgtagacgt attcatcatt ggttgcctgg ttgctgtacc
    20341 agatgccgcg atagccgtcg tcggggccga tggatttgag cgggtcctgt gccgggcgag
    20401 ccggggccgt tgattgtccc cgcgcatccc gggctgttgc acagaaagcc aggagcccgg
    20461 tcatcagcaa acaagtggga agcgttcgac aagcaaagcg agcatgtctc ggttcaactg
    20521 acatgtggtg gtctcctctg gagcatgcag atggcaatgc gtaccaacga tgatgacatg
    20581 ctaaggatct ggtcgtcccc tgcaaggttt gggagctgct cggcccggaa tcttttccga
    20641 accgcctctc ctgaaagact gctcggcccg gaatccgcct actgctcggc ccggaatccg
    20701 ttccgggccg gttcgcctgg ccgaatccat tccgcatctg gtgagaatga ggataggaat
    20761 cctccgcgcg gaaggcccca gaagggattc tggggccagc gaaatcctcc gcgcggaagg
    20821 ccccagaagg gattctgggg ccagcgaacc cagagatgat tctggggcca gcgaacccgg
    20881 agaggattct ggggccagcg aacccggaga ggattctggg gccagcgaac ccagagatga
    20941 ttctggggtc agcgaaaatg aatctgcggc cagcgaagat gattctgggg ccggcgaccc
    21001 cagaaatgat tccggggccg gcgattgcga ccggacgggt cccattctca attctgcatt
    21061 cgccgttctt cattccccgg cgtagtcgca tgcaacgccg gggttggggt atcaagtctg
    21121 tggagccgct cggccacctg cccgagccgg atcgctgctg cttcgaacgg tcggaacatc
    21181 gcgccgccga cttcttcgag cagatgccgg tcgatcgctt cggccagcat ctcggtgccg
    21241 gatcgggcgt cgaggtgttc ggctcgtccc cgtccgcctt cccgcaacag gccgcgtgcc
    21301 ctgtagacat cctgcacgct gcgggcgtag atcgccgcca ggcaggcgac gtagattccc
    21361 acaaccacga cgaagccttt cagcagttgt gtcgccccga gtgcccagac cagccggtac
    21421 aggccgtgcg cccagcccat gattcgtgtc ccggcggcgg ccatctccag cagaccctcg
    21481 gccaccggct gaacgaacgg gaaccagaac agcacgcacc acacgaacaa ccgtttcgcc
    21541 caactcggcc ggtagccgat ggtcaatcga ttgatgatgc tctcatcatc tttgaggggc
    21601 aactcggcca gctcctgctc gactcgcgcc gcaagctcgg ccgtgtcggg gaatcgctcg
    21661 cggagctgga attgccgcac aatcggcgaa tgctcggttc gcagccgctc gccgagagcc
    21721 tcgaggcggt cagccagacc gcggcttcgg aatcggaaca gttcctcggc gggaacggcg
    21781 gtctgcgggg ccgccgtccc cggcccagca agcgttctgc ccactcggcg gattacccag
    21841 cgactcagcc agtacgcgac tcgcagcagc ggccagcggc ccgcgcggac gtccatgacc
    21901 tcgtcggcca gctcgatgtc cgagcggcgg gtacgccaca gggcagttgc gacccctcgg
    21961 cagtagtcgg cgccgaatgc atcgttgagc atggcctgcc gatgctcgcc ggcggtcgct
    22021 cgttgcagtt gttcgagcca atggtcgatc tcataggcct gccggatccg ttgcgtgtta
    22081 cggcggatct gctcgcgcag gttacgttct ttgacctcgg cagcctcgtc cgcgccgagc
    22141 ggtccgagca cgttccggcg cagccgcttc acttcgtttt ccaggaaccg gccgatcgcg
    22201 gtcactacgg gacggtcgcc gccgtaacga tgccagccgg ggtcgtccca gatcactccc
    22261 acgcgttcga gaaaggccga cgactcggcg aacttgacgg cgaggagaaa gacgcggtcg
    22321 gggttcattt cgtgcccggc ctcggccagc attcgcagtg cccactgtct ttgccgatta
    22381 cagaacattt cggcctggcg ctcgatgccg gcggcgtcgg acgggcgggt ccagtcttcc
    22441 tcatcggcca gcagttcgtc gcacttgttc agcacgaagt acacgttcgt atcgtccttg
    22501 accacgtcct gagcgaatgc aacccaggcc cgatcggcga tcttgcgagg cgttgcgacc
    22561 cagatgacgc ggtcgagatg cggaaccacc gccttgacga tcgcctcgtg ccgcatgaag
    22621 tcgctgtcga agtcgggcag gtcgacgagg acgatgttac ggatgccgtc ggcggaatgg
    22681 gtggacacct gcagatcgag tccgccgtcg acgcggccga gtcgcatgcg ggcggggggc
    22741 accatgtcgt gatgaacgta cacgatgggg cgcgaagtgc ctgctccgac ctcatcgcgg
    22801 tcgatggaga tttctttttg agcgagcgtg ttgaccagcg ttgacttgcc gacgtccttg
    22861 ccgcccagca gtccgcagat gaccaggggg tcacggcgtg actcatctgc tccgcccgcc
    22921 agcttgatcg gttcccgccc gaagagggcc gcaagctcgg cgttgatccg gtccagctcg
    22981 aaggcggcgc cggccagatg gtcgccgtct ttcggtatgt cgggtgctcc gttcatgctg
    23041 atcgatcctc ttgtcggctc agaatccgca gtgcctgccc gagcgggtca ttcgggggca
    23101 aggtgaacgc ccgggcttgc tggctgaggt agtctgcggc ggcccggcca tgctcctcga
    23161 tgagacgccg catgctctcg gcggcctcgc gtacgcggcg gaactcggtg ctggccagga
    23221 gcttctcgcg aatgatctcg gtgaggcggg cgagcggtcg tgccgagatc gccccgaatg
    23281 ctcctgccgc tcccagcttg agcaggccgg ccttaagggc cccggccgcg atgaccggcg
    23341 tcagcgccgc gaccggaccg ggtacggcga tcaggatggc cgcccctgcc aggatggtca
    23401 ttcccagcga gccgatgagc ttgggcgaaa tgccctcgca ttcgtgttgg accttggcgt
    23461 tccattcatc catcgcgacg gccaaccttg ccacggtctc atctgcgcga ggccggaatg
    23521 actcaatcag tccgcgatta accaggaggg gcggccgacc gtggacggtt cgcagatcct
    23581 cccagaagtc gctcgctgtt acctcctcgt tgagtcggcg aatctggcgt tcgcactggg
    23641 cgttgagcca gtcgcggcct cgttcctcgc gggtggcatc ggtcttctcc gacaagggcg
    23701 gggccgcccc cacggctgta tcgcgtttgc cggtccaagg cagccgcggc atcacctctt
    23761 cgagccagcg tttttgccgt ccgagccact ctcgcatttg cgaaatcccc agaatccggg
    23821 agacaacacc aagccgatcg cgttcttcgt gggtcatcag ggcggtcacg caatcccagc
    23881 gggttgccgg ggcgcagcgg gcagccacgt cggccaacgt gcgttcgagc tgatcgagtc
    23941 gttcgaggcg ggttcggttg gccgacaggg cggcggcagc ctctctggcg atctcggcgt
    24001 gcagcgccgg ctctcgccgc ggcggcggtt cggccagaaa tctcgatatt tcggtgagat
    24061 cctgcggatc gaagcggcgg aaaccgcgtc ctcgatgata gttcaggatg cataccttct
    24121 cggcgcccag atggcgtgcc tgttcttcga gtcgcgagcg gtcgggctcg gcgaggacct
    24181 cctcagaggt ccgattaaag atcaccgccc gcggctgggc gaaccgcccg gccagcaggg
    24241 ccaactggtc cacaacctgg cgatcgaacc accgttcgtg gtcgacgatg accaccagcc
    24301 gatcgaacca tggcagcagc gatagtgtga cgtcgccctc gcgttcggcg gtgctggagg
    24361 tgaagtcggc cgtgtcaatc agcagcgtgt tctcgaaacc ggcgacgtcg tgctggacga
    24421 ctgtcgccgc ttcgggccgg ccctggaggt cggcgtcgag acggccgggc tgagattcga
    24481 gcgtcggtag cagaatccgg tcatgattca accagcggtc gagccggtcg tattgcttct
    24541 gatgagcagc gacgatcaag cctcgcgtcg catggctgcg gatggtcacc aggctgacgc
    24601 ggtggccgcc gagcaggttg ttgaagacgg tgctcttgcc cgaggaggcc cctcccacga
    24661 tggccgcgag ttggaccggc tgtcccgcgg cggcggcctg acgacggaat cgcaaggcgc
    24721 agaccgcctg ctgagcctgc tcctgccagg gcagacaaag ccgctcccgc aggcaccggc
    24781 cctgctgggc cacgacgttc agcgcagcgt ccaggtccat ggtccccaca ttcagttacg
    24841 gctcgggcac ggatattctg cccgtaagtt ggcagtcgcg ggcattcgcc ttcgccggtg
    24901 atgcaccagc ctatgcgcga cagggagttg catcaactgc cgggcgtctc gccgtggcag
    24961 agggcagggg caggcacatg ttgcgcagca gcttccctca ttttcgcaga tcgcgaagca
    25021 cgatgagatc atccaccgtc agggcgcagg agaagcgata cccggccagc gtcttgagcg
    25081 gtcgcagggc cggcaactcg caatacgggt gatcgaacac tttgagaacc gcatcacctt
    25141 tccacgccag cgatacctga acgtcgcggg acttcagttg caccagttcg tggacggccg
    25201 gccggtcgtg gcggcctgcg accagctcag gaaagcatcg cacgttgacc gccctggcaa
    25261 agccgggcga cggcagttgt gtggtctgct cgcgcagcgt gaccaacgct tcagccagcc
    25321 gccggtcttt agtcgcgagg gtggctccga accggccgcc cggaccgagc attggcgccg
    25381 ccttggacac cagcggggaa ggccgagtga tccaggtcga gccgatctgc ttgggccatc
    25441 cttgaatcat cccgcgcacg agcgaaacgt cctgatcgac ccagataaat ggacagtagg
    25501 agcagggcga gttctcgtgc gacgcagaga gcaggaagat cgtctcgtga tacaggctgc
    25561 gggccggatc gagatattcc tcgccggttt cgctcgcgta ctgccattcg atgaagtaga
    25621 cggcgcattg cggagagtga aactccaggc ccgccggcag aaacgaccgc acggcctcgg
    25681 cgtcggcctc gtactcgatc gccagacagt ctcctgcgaa ataccacggc ggatggggga
    25741 cgacgctgga tgtccctttc ggcgtccgcg gccacgtata acctttcagc atgtcgttct
    25801 ccttctgaac tgctcgaatc accggcggcc atttcttaca gcggctggcc ttcgctggcc
    25861 ccagattcca atctggggcc gcatggccgt gacgattccc atcgtcagtc acacggatgc
    25921 ctcagtccgg tggggcgtgc tccgcgccgc cttctcgttc ccacaagtct ctgccgctca
    25981 ccttttctgc cggccgttgg ggggttacgg cagggcatgc cacgggcttc agaaaccgga
    26041 cagaagtact ggagttcctt aacctctcgt gaactgccac agaacaacac tcttcgaagg
    26101 cacagtcgtc aacccgcggg cgatcgtcat tcagagtcac gtaatttacg cattggccga
    26161 gagttatcaa gggcaatcgt tgcgattgat gaacttgccc ggcaatgcga cgtcagattc
    26221 ggctgttctg cggggaggtc tttggcctat gcgagaatgg gacgtgcgcg tcgcactctc
    26281 aggcacgatc gccccgcatc cgacttgttc gcagtcaaaa agacttgtat tgccgcaggc
    26341 aggcggtaga atacttatta cgttgacaag aggagaggcg agagcagtct catcagtcgc
    26401 cggtttcggc tagctatgtt actggccccg gccgctaagc cgctcgaaca agaagacgag
    26461 ggagagaatc atgtcacgtg tttatcactc gtgggcgatg ttgtctgtcc tggttgcgat
    26521 ggccttctgt gttgggagac ctgcagtcgc aattgacatg tactggactg gagagggtga
    26581 taacaacaac tggtactcgc gtcacttcac cggccagtac gactcagagc accgtcccat
    26641 ctacgtgaac aactggggtc agatgggtat cgatccgccc acaccgggcg caagtgacca
    26701 cgcgatcgtg ggtggggact acaccgccga gatcaactac cagaatggcc cgtccatctg
    26761 ggagctcagt gttggtgccc cggcgaccct ctggatcggt aacgccggca gcctgacggt
    26821 caacggtcca tccattttca atcagggcct cattcagatt gcctacgtcg gctttgccgg
    26881 cggcgggacg ctgtcggtca ccaatggcac cgtcaccggc ggcggagcta tcgagttcac
    26941 cggagcgggt acccttagcg gggcgctgac gctcgagtcc ggcaacctca ttcacggctc
    27001 gggcgggcat ttgaccgcga cactgctcaa ccaaggcctg gtgagcgccg acgtcagtaa
    27061 ctcctggctc agcctggaaa ccggcgcaaa gaccaatgaa ggggtattct cggcatctgg
    27121 cggtggtcgt ctcgatgttc gcacctccgt ctatcaaacc agcctgggcc agatggtcgc
    27181 tggagacagc tcgaccgtac agtactataa cggttcgtcg gttaccggag gcacgaccga
    27241 gtcttcgggt acgggcaagc acttcatcac cggccagaac gtgaccacca cgctcacgga
    27301 catcaccaat acgggccact ggctggcaca ggatggttcg tttgtgaacc tcgccggctc
    27361 gaccttcacg aacaacggca ccttcgaggt gggcggttac atcggcggtg ccggcatggc
    27421 caccctgagg gtgcaggcgg atgtgacact ggcgggaacc ggcgatctgt ttatgaaccc
    27481 tggtatcgtg gacagtgtga ccgggtgcac gctgacgaac tccgccactc acaccatcca
    27541 cggccgtggc aacttgaagg ccgccctcga caaccagggc caagtcattg tggactacaa
    27601 cggcggcacc ctgagcctgg aaggcgccgc gaaaagcaat gaggcgatga tcaaggccac
    27661 cggcggcggg attctggccg tcaagacggc cattacgcag tctgcctccg gtcaagttgt
    27721 cgtggagaac aactcccgtg tgcagtatta ccatggttcc agcatatccg gcggcacgac
    27781 tgcgtcttcg ggtacgggcc agcacttcat cacaggccag aacgtaacca ccacgctgac
    27841 ggacatcacc aatacgggct actggctggc acaggatggt tcgtttgtga acctcgccgg
    27901 ctcgaccttc acgaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaacg
    28021 gcaccttgga aatcggcggc tacacgggcg gaagcggccg ggccaccttg acacctgctg
    28081 atggcatgat tcttcagggc tccggcactc tcttgctcac cccgggccat atcaatagcc
    28141 ccgcgccggt aaccatgacc aacgcagccg gacacactat cagcgggtcg ggcggtcgtg
    28201 tttacagcaa tgtcaccgtg aacaacctcg gcacgatcga ggctaacgga ggcaccgttg
    28261 aactgcagac cgccccgaca cagttttccg gcaccacgct caccggcgga acctggaagg
    28321 cgatcaacgg cagcctgacg gtcggcggct ctccggccgt aacgaccaac caggccaccg
    28381 tcgtactcag cggctcgtca tcggcgtttg ctcccgtcaa ctcgctggct gacaaccagg
    28441 gcagcttctc gattctggga ggccggacct tcaccaccgc cggcgcgctg accaactcgg
    28501 gcacgatcga aattgacgtg agcagcaagc tggccgtcaa cggcggctat acgcaaaccg
    28561 ccggtttgac cagtgtcgac ggcaccttga gcacaacgac gggcatggtc ctctcgggtg
    28621 gaaagcttgt cgggaccggg acgatcaatg gtgcgacgac cgtggatgcc ggcgccgcgc
    28681 tcagccccgg ccacagtgcc ggcgtgttga ccttccagag gctctcgctg aagagcgggg
    28741 ccaccttcga cttcgagatc gacggaccca acgccgaccg gatcgaaatc attggctcgg
    28801 gcgatgtctt cactctcgct cccggcggcg tgttcacgat caacctggat gtcttgaatc
    28861 cgacgggcgc gctgagcgag tacacgcttt tccactgggt cggcagcaca gccatcccct
    28921 ccgacacgac gtggctggtg acgggtgccc caggcgtgag cggcgttgtc gagatccggc
    28981 cggacctcaa tagcatcatg ctgaccaacg tgatcgtccc tgaacccctt tcgatcggtc
    29041 tgtttgtcgt tgcggccatg ggcctgccgg cccgccgtcg gtcccgctga tcgaacaggg
    29101 atcgggatcc ccaatgatga tttgcctcgc gcggcccgga acccgcacgt gtttagcgtg
    29161 caagacagcg ggtcaggcac tcttcgccgt ctttttcgcg ccgccgagcc cccgcctgac
    29221 tgagccaacg accctcaaaa aggggacatt ctacttttcc tagaaagcag aatgtcccct
    29281 ttttcagccg ggtggcccag gttcttcctg aacctgggct cgcgattctc ctgtgcaatg
    29341 ggtggcatgg ccaagccccg gcttgccgga gcgccggcat gttctggtag ggcgtgttcc
    29401 acacgccgcg cctaaccgtc tgatcgttcg atagactaac agactaacag gctaacagac
    29461 tcacagacta tctgctctcc cctgttgccg gccgtccatc cctcacgccc ggcagacgat
    29521 cctggaggtt gagcacgttg tagcggcggc aaatctcgac cacgcggtcc aggggaatgg
    29581 ctcggcgatg gtggccgtca atgccggtca ccgatgtggc ctggaacatg gagttgtaca
    29641 ccgcctcctc ggtcgcttcg acgaccgcca tgaacaacgg gttcatcgca cggccggcga
    29701 cggtggcgac gggcggatcg agcacacgat cgtggtcggt gatgcggtag gcggtcgaga
    29761 atgcgatgac atagtcgccc gaaccgttct gcatcgacga gccggtccgg cccaggccca
    29821 gcacggcgcg tttggccaga cgttcgaggc cgtggggtga gagtggagca ttggtggcga
    29881 gcacaatcat gcacgagccg tcgccgccgg atgcggtcgt gtactcgctc atcgagaagt
    29941 cgccgagttc gcggcccacc ggggcgccgt tgatggtgag gacgccgccg tagttggatt
    30001 gcacgaggac gccgacggtg tagccgccga gatcgtgcgg gagccggcgc gaggaggtgc
    30061 cgatccctcc cttgaagccg aagcaggagg tgcccgtgcc ggccccgacg ctgccttcgg
    30121 cgacaggacc cgaggcggcc accgcgatcg cagcaagggt atcctcgggc gtcacgtgca
    30181 tgccgcggat gtcgttcagg tagccgtcgt acgtctcacc gacgactgcg ttcaccgagc
    30241 caacggtctc gttgcccggt tgattgagcg tgtagcggat caggccctgc atgcacgctg
    30301 cgacgcccag cgtgttggtg agcgcgatcg gggtctccag cgttcccaac tcggcgattt
    30361 gcgtcgagcc ggcgagtttc ccgaaggcgt tgccgcagta cacggccgcc ggcaccttct
    30421 cacgataggt attgccgtcg tgcgggacga tgaccgtcac gccggtgcgg atggattcgc
    30481 ctcgccgcag ggtctgatgc ccgaccttga cgccgggcac atcagtgatc gcgttccact
    30541 ggccggtcgg caggattccg acctgaatgc ccaactcccg gcagcgggca cgcttctcgg
    30601 cctgcccgac ttgtcggtct gcccctggcg ggtcaaccgg ctgtgcgaca accgccgagc
    30661 cataaagcaa tactgccaag acaatgccag caaccctgca tttcatagtt gatccatcct
    30721 ttccggcaaa gcgtcgtgcg ccccgggaca gcctttgccg aagggctacg ttgcgcacgc
    30781 cgctatgcac gccacggtgc cgacagcttc tccttgccca atagtccgag gcggagcgcc
    30841 cttttgcggg ctgcaaccca aagcggcctt tggccgcaac caaattgaag aatcagcgag
    30901 caccccacac atccccttcg gacgggtatt gacgtgacgg ctcgaaatct gcccgctgct
    30961 ccgaggctcg cacgcgcgtc gttgtgtcgg gcgcattggt ttgcgggcca ctggcaagac
    31021 acatcccgtc cgatctcaac gccgcgatat caaacgatta cgaagaaggt caaatcatct
    31081 tcgccggctg catcttggca acctttatct gacggcactc ggcgggaatg ctccatacca
    31141 ctataccatg aacctccgag accccaaaac atgaccgcaa aatgcagaga agttccggag
    31201 taagggacca aggcggttcg cgtcagcttc ctgctctgag ttgtgagcca gacggcacgt
    31261 acggggcgag agaagtgcga gcggcaagga aggagtggag ataggggtgg atgatgcgac
    31321 gggcagatgg agtatcagtt cacagtggtt ggtgatcaag ggggcggcag gtttggcaac
    31381 ctgtcctgcg ggattgtggg cacaattgcc tcgccaaccg aatgcgccgc cggtctcgag
    31441 acgcgccctg gaaaacgact cggttcccca ccccttgtgg gatgggggat ccggggctat
    31501 ccggaaacgc tgaaagccga ccggttggaa accgatgtca cagaggtttt gggataactt
    31561 ttcatgcttc gggctcggcg aagagagcct ctacgaagct ttccggattg aagggctcga
    31621 tgtcgtcggg ggtttcgccg aggccgacga acttgaccgg gatgttgatc tggtcgcgga
    31681 tgccgatgac gatcccgccc ttggcagagc catcgagctt ggccagaaac aggccggtga
    31741 ccttaacgtg ctgactgaag accttggcct ggttgatggc gttctgaccg atggtcgaat
    31801 cgaggaccag caggacctcg tgcggggcac cggggatttt cttttccacc acgcgctgaa
    31861 tcttgttcag ctcgcgcatg aggtgttcct gggtgtgcag ccgcccggcg gtatcgacga
    31921 tcaagacatc ggcgttgcgg gccagtgccg cctcgcaggc gtcgaaggcc accgcaccag
    31981 ggtcagcccc ttgttggtgc ttgacgatct gaacgccgag ccgcctggac cattcggtca
    32041 gttgggcgac ggcggcagcc ctgaaggtat cgcaggccgc aaggatgacc tggttaccct
    32101 ccctggtcag atagttgcat agcttggcga cgctggtggt cttgcctgag ccgttgacgc
    32161 cggtaaccag gatgaccgtc ggtccggacg cggcgcggat caactggcgg tcaggctcgg
    32221 gccaatagga cacgatcttc tgtttgagaa aagggatgat ttcctgcgtt tcgcggatct
    32281 gtccgctttt ccaggcgatc cggacctcat cgatgagtcg ggcggtcacg gccgggccca
    32341 tatcatcggt cagcatggtc tcttcaagct ggttgatgat cgtatcgtca atgttccggc
    32401 caaacggcaa aaccgatcgg aagctccgcg aaatcttatc gcgggtcttg gccaatccct
    32461 gcttgagtcg atcgaacaaa cccatggtca gaccaccagg cctttagacc tctagactat
    32521 cggactgaca ggctccggct aaaagctaat cgccgagagc ttctttctca caccggttcg
    32581 cctcttgcga cccggcaggc cggatgcttc ttccagatcg cgtcgagaac accgttaacg
    32641 aattgaggcg tctcggcatc gccgaattcg cgggcgatct cgaccgcctc gtcgatcacc
    32701 acgcgagcgg gcacttccgg ccgttcaaca agttcgtata ttgccatccg caacacgttt
    32761 cgatcaacaa ccgccatccg tgagacttcc caatgatccg tgacctcggc gatcatgcgg
    32821 tcgtactggt cgcgtttggc ccacgcgccg gcggtcatct gccgggcata gtcggcgacc
    32881 tcgggggatg cgcctgcttc ctccagaatc ggcttcagac actcggccgc atccgcgtca
    32941 gcgggcgcac gctggatgtc cagttggtat agcccctgaa gggctgccgt tcgtgcctga
    33001 tgtcgggatt gcttcatcgg ggcgggcggt tcaggtaatc ttgccttcga gtctcgcgta
    33061 gaggttggcc atctcgattg cggtcatcgc ggcgtcggcc cccttgttgc cggacttggc
    33121 tccagcgcgg ttgatggcct gttcgagcgt atcggccgtc aggacgccaa agacgaccgg
    33181 tacctcgacc gacaagccta cctgggctat gcctttggca gcctcagcgg ctatgtagtc
    33241 gaaatggggg gtctcgcccc ggatgacgca accgaggcat atcacggcgg catactggtt
    33301 ggtcgcggcg atcttgcggg cggtcaacgg cagttcccat gcacccggga cgcggaccac
    33361 ggtgatgtcg gacatggaac acccgtgccg ttcgagggcg tcgatcgccc cgtcaagcag
    33421 gcgtgagcag acaaactcgt tgaaccggct ggccacgatt gcgaacttct ggccctttcg
    33481 gaggatcagt tcaccggtaa ttgtcttgac gttttctgcc ataaggctga ctcccacggt
    33541 gcgacggctt cctgtcctcc tgaaccgccg gcaaccggac gctgtgtata ggcccttcac
    33601 ggctgtcttg ggcgcgggac tgtatggtac ctgagatggc ctgtggatgc cagaaccaga
    33661 atccggactc ccggtggtca cgtcggccaa accatcaagg aaccgatcgc gcgcggagct
    33721 ggtatcgctt tgtcgggctc atgcccttat cctgcgactc tgcaggtaga gagaaagaaa
    33781 tgtcaggact gccctttgtt gtcgacgttt gcggcctgtt cggcccggat caactgcgga
    33841 ttgtgtatcg ggacgagccg gccccggcca tacccgccct ggaggccata gtggcagatg
    33901 cctgggccaa gcagatggaa cttgcccggc aacggggctt tcatctctac aacggccaga
    33961 tcgtccgcct gctgaggtac cgagtccaaa aaggcctttt catgatggag ggcgggccat
    34021 ccgattttgc ccattttatc gggacaaatt acctgaatta ccatcgggct gacgaattcg
    34081 ggtggacggc gttcagcaac cccattggcg tgtcgggtat ccttatcacc ggtgacggtt
    34141 ggattctcta tggtcggcgt aattatcgag tggcctgcca tccgggctac gtccaagcgt
    34201 tcgggggcag ccttgagatg ggggaacgcc gggcagacgg cacctttgat gcctttggct
    34261 gcattctgcg cgaacttgag gaggaggccg gtgtccagcc agaggatgtt gccgagacgg
    34321 tatgcctcgg catggcccgt gaccgtcaaa tccggcaacc cgagcttatt tttgatgtca
    34381 agatccgcca gggacgtgag gagatgcttg accgccttcg tcacgatgat gccgaacaag
    34441 agcatgcgga gatcgttgcg tgtcaggacg ccccggaggc catcgtgccg ttcatccggc
    34501 ggatggtgcc gatgtcgcct gtggcaattg gcgctctttt tctgcatgga tggcggcatt
    34561 ttggcgatcg ttggtacgaa caagcttcac aggatttata tcagccgcaa tgacgtcctg
    34621 ctggtcactc tcgggatgcg aattcctcca gcctgttttg ccggaccgcc gatatcttcg
    34681 tattggggca ccgcgctgcc caaggtggtt ttttgcgccc gacaagcgtg gttgagcgtc
    34741 ctgacggttc gccgcccaac cttggaaggc ttcatcttgg gcggtaagat ggcggccgcg
    34801 tatcacggag gaagcgctcc gcatgaacaa gttccagcaa cgcctgcaga tcatccgtgg
    34861 ttgtctgatt ctgctggtgc tgatcagttg tctgttcctg tgggtatcag tactgagttt
    34921 cagtccactg gacgctccga atccgcgcgt gtacccccat cctgcgcagg cctacaacgc
    34981 cgggggtaag gccggcgcct gggtggcgta caagatgttc tcgtacttcg gcaagggcgc
    35041 gtatgcggcg gcggcggggc tgacgatgac catcattgtg ctggcctgga aggggtgcct
    35101 gccatcactg tggcaacgta ttatcggtgt cagtttgttg atctcggtca cctcggccgt
    35161 ctctcacatg ttgttggctt ctcccgaccg tttccgcgaa gagaccggcg gcatactggg
    35221 ctattgccta gccacctggc tgcaacagtc ggttaattgg atggcttggc cgatgctgtt
    35281 ttacggcgcg gtagtcggtc tgctcttcac cgccgaggaa ctggttctgc aaatcccggc
    35341 catgttgtgt cggcggcgcc aacaggtcac ggagatggcc tccgcggttg ctcccgccgc
    35401 ccagaggatt acatcgatca ccggcccctt tcgccgggca gtcgcgaggg tgaaaggtct
    35461 ttggccgcgg ccggctcctg ccggggcacc gaccggcggc cggtcttatg caccggtgat
    35521 tgtcagaccg aggccggcgg ttgccaagcc caagcctgct ccgagcaagt ccgcacccga
    35581 ggataggagc gagcagcagt cagagggcga tgatgagccg gacgaggcgg aggccaaggt
    35641 tgagaagacc gcctcggccg tcgcgattga agagaaggat aatgaaaccg ccccgcccga
    35701 ggcgggtgat caagatgagc cgctcctacc cgcgcctgtg gcaaccaagc cgccgccaat
    35761 ggttgtgaac tttcccacgc cgcccaagcc cgcggctgtg ccccagacga ttgtcccccc
    35821 gaagccctat ccggcggagt tgggcgagtg gacgttcccg cccctttccg cgcttatcga
    35881 tccggagtac agcttcacct cgcagcagga ggcgttggtt cgggagaaag cccaggatct
    35941 tcagagggct ttgcacgaat acggcatcga ggcccacgtg gtgaccatcg acaccgggcc
    36001 ggtcatcacg atgtttgagt tacaggtggc tccgggcgtc aagctgagcc aaatcacctc
    36061 gttggccaac gacattgcca gggcgcttta cgctcccagc gtgcgcgtgg tggcgccgat
    36121 accggggagg aacaccatcg gcatcgaggt gccgaacctc aacaaggaga aggtccgcct
    36181 gaaggaactg atgacgatgg gcggcgacgc cgttcgtcgg atggccctgc cgctgtttct
    36241 gggcaaggac gcgagcggtc gggcactgat cggggacctc gccgggatgc cgcacatgct
    36301 gatcgccggc acaaccggtt cgggcaagag cgtgtgtatc aactcgatca tcatgtcgat
    36361 cctgatgact cagcggccgg acatggtgaa gctcatcctc gtcgacccga agatggtcga
    36421 gatgagcacc ttccgtgacg tgcctcatct catgtgtccg atcgtgactg aaatcgaacg
    36481 ggccgagaag atcctgtcct gggcgacgga gaagatggat gagcggtacg cgctgctggc
    36541 ggaggcgggg gtacggaaca tcgcggctta caacaagctt tccgagcagg agaagctgga
    36601 gcgtttccag ccgagtacgg aagaagagaa gacgcagatt cccaagcatc tgccttacgt
    36661 ggtcatcatt gtggacgagc tggcggacat gatgatgatg tctgccaagg aggttgaggc
    36721 tcacctggcg cggctggcgc agaagagccg cgcggtcggc attcacatca ttgtcgcgac
    36781 gcagcggccg gaggccaaga tcgtcaccgg cctgatcaag agcaatctgc cgtgtcgaat
    36841 tgcgtttcgc gtcgcctcgc gcatggattc tcgaatcgtt ctggaccaga acggggcaga
    36901 ggtgctcatg ggccagggcg acatgcttta cctgccgccc gggtcctcga agctggtacg
    36961 cgctcagggg acattcctcg aggattccga ggttcgcgaa atcatcaact tccttcgcga
    37021 aaggagcaag ccggagtttc acccggaact catgcaactc ggcaaggcgg aggcatccgc
    37081 cgacgggccg cgtgatgaac tgttcgacca ggccgtggag atcattctgg agaccggccg
    37141 cggcagcgtg agcctgctgc agcggcggct ggccatcggt tactctcgcg ccagccggct
    37201 cattgaggaa atggccgctg caggcatcgt cggcgagtat accggcagtc aggctcgcaa
    37261 ggtcctgatt accgctgagg aatggcaagt ccgcaaggag caagaggcca cccgcatccg
    37321 ccaggccgct ctgcaagacg acggtgtgcc ggatatgctc tatgatgacg atatcgactg
    37381 agggttcggg atccggggtt cagggttcag acaaggcagg gcacggtcgg agtcgccgga
    37441 ggtgacgcag acctgccacg ggttttgctt caccctgggg cgggtctcgt ccgccgcggc
    37501 ggactcgaca ctgcccgacc gcccggacgc ttcgatcttt acacactcgc ggaaccactg
    37561 atttgctact ccgatacttc cacctccaca atgctttcct cgcgtaatcg ttccgccggg
    37621 atgaaaccat cggggggcaa gtcgcggccg tcgattcgga ggctggtcac cttgacgccg
    37681 taacctcggt agtgaaaggt gaccttcgag ccgtgatagc ggattgagaa cacagagccg
    37741 ttgagccgcc ggggcaagcg tgggcggatg gcgatgccct tcgtgaaatc gaaagtgagc
    37801 ccgaaaaggg acatgaaccc ccagacggtc acggccacgt tggccagata aggttcctgc
    37861 ttgctgccct cggtcggcag ccctgtctcc gtatcccaga agtagccgtt gccggcgccg
    37921 tggaggttgt cgcgctcata ttgattgaga atggccagat agtgatcgta ggccttgtcc
    37981 gggtcgaatt ccgccatggc agcgatgtag tatgcgctga cccacgggaa gatgccgccg
    38041 ttttcgtagg taccgaacag gtcgtagaaa tgcggatagc cgtaggtctc gagccaccag
    38101 tcgccggcgt tgtagtgctc gggcgggatg ctgaccaggt tacaggggag ggagaagccc
    38161 ttgtaaccca gttccgccac gcggcgatca atggaatcga ggatccgccg acgcatgggc
    38221 tgatcggcga tgctctcggc ggcggcgatg aggttgaccg aggtgaagaa gtagtcgtgg
    38281 cggcctccct gcgtgtccac ccacgagatg tatcggcccg cccgctcgtc ccaaaacagc
    38341 tcgttaaacg actgccgtaa ccgtcgcgcg gcggccgcgt aatgatcggc cagcttcacg
    38401 ttgcctgcgg cccgttccat ctcggccagg tcgcgtagtg cgggatacgt cagcacgttg
    38461 atgaaggcgt cgtagccgcc gcaggaaacc acgtcccacc agttgcagac gcgggcgccg
    38521 ttgcccgggc cgggaatacc cgtggaggtc ggggtcaggt agagaaaagg tctgtcggtg
    38581 caattggcca gtatgtactc tgcccatcgt tcgccgtact tcaggcggca gaggtcaccc
    38641 gttcgtcggg catagtcggc gaggaagata agcatggaag cgttgttttc atggaaggcg
    38701 cctcggccgt cgaagccgta tccccagacc agaccgtccg accgggtctc tcgctgatac
    38761 acggcatcga gggcgcgggc catgacttca tcgatcgcgg ggtgcaagcc gaagaaatgc
    38821 gccttgccgt gcaggtaata ttcaaaagaa acgccgtggg ccctggggtg ccccacgttg
    38881 tgccatccgc cggcaaaacc ggcgcggtgg gcgatggagc cgtgggcatg cgtgcgatga
    38941 aaccgagtcg tttgcttccc caccgtccgg tccggtgttt cgagatgcat cgtctcgacg
    39001 ttctttggcc gatgtgcctg tatcacaacg ctacactgaa agcgcgtttg cggttcgacc
    39061 acttggtttt ccgaccatcg aggagtgccg atgcagaacc gctcgtgaga tttgaagaca
    39121 aagcgggccg gcggcagtcg gcgcgggtca cgcggccccg cgggcctgca cgtcgccctg
    39181 ggtacgatcc ggaagagatt gtcccccgcg caagccacca gcaggccgta tccgcccgcg
    39241 aagttgaggt acatgatttc gtgcccgtcg ccggcaaact tcagccgcac gcggcggtcg
    39301 agaacaccgc ggccgccttc gttgccgcgg taggtcacct cgggttcagg gacggcgaac
    39361 cagtgcttgg gcggcaggat atagccggtg tcgaaccaag cgtcggcaat ctcttcgtct
    39421 tccggcacgt cgaacgccag caggttgatc tcgtcgtccg gcagggtttt tcggccgacg
    39481 tgtaccgaaa catcccaccg caggtcgtcg tttgccaacc ccagggtcca gtagatctcc
    39541 caggatgcag cgaacagcgg cccgtaccgt acctggcaaa cctggtcgtc atcacgaagg
    39601 acatcgaccg tgggggattg cggcccctcg gtggtgatga agaactcgcg ggtgtggctg
    39661 gtgacgccgg cgaaaccggt ccatggcagg agggaaatgc gaatgtcgtg gcggccgtcg
    39721 cggttgatcg acgcgcaaaa ctcctcgaca agccggcgcg taccgggacg agtgctcagc
    39781 cggattctga aagcgcggct cgaaaggacc actgattcgc tttcacggac aatcatcgtg
    39841 gcctgtgtct cccgctctga acacccctcg acctccgcag cggggatact ttacagcacc
    39901 ggcgccggct tatacagtgc cgaggactgg gaggaacaag cgtgccggaa ttgagccaag
    39961 acaatctgcg agagttcgtc cgccgtgtgg catctgagac tccggttctc gacattcaca
    40021 cacacgtgta ctccgcgcga ttcggcgggt tgctgctcta cgggctggac gaactgctga
    40081 cataccatta tttgatcgcg gaggtgtttc gcgttgcccc tctgccgtac gaacggttct
    40141 ggggaatgtc gaaatccgag caggcggact acatctggca acacctgttc atcgagcatt
    40201 cgccggtttc cgaggcgtgt cggggcgtgc tgacggtact tcaggcattc gggatcgacc
    40261 cgaagcagaa gaatctgaca agtatccgga agcacctttc ggcgattccg cgagaagcgt
    40321 acatcgacca agtgtttagg ctgtccaatg tcagacgggt gatcatgacc aacgacccgt
    40381 ttgacgacaa agaacggccg gtctgggacc aaggccccgg gtcagacgag cgatttgagg
    40441 ccgccttgcg aatcgacccc ttgctgaatg attggggttt cgccgtcacg cgcttggccg
    40501 gctggggcta ccgcgtgaac ggcgagctgg gagcttccga cctgaccgaa gtgcggcggt
    40561 ttctaaccga ctgggtcgtg cgaatgcgtc cgcgctacat ggccgtgtcg ctgccaccgg
    40621 atttcaccat ccccgaggac agcccgcggg ccgccctcat cgccgggtgc gtgctgcccg
    40681 tatgtcgtga ggcgggcatc ccgttcgcga tgatgatcgg cgtgcggcgc agcctgaatc
    40741 cgcaactgcg attggccggc gacggggtgg gcgccgccga cgtggccagc gtcagccggt
    40801 tgtgcgcgac ctgcccggac aaccgcttcc tggtcaccat gttggcccgc gagaaccagc
    40861 atgagctctg cgtcgcggcc cgcaagcacc ccaatctgat ggtcttcggc tgctggtggt
    40921 ttctgaacaa ccctagtctg attgaggaga tgacgcgcat gcggctcgaa ctgctgggga
    40981 cgtccgtcat cccgcagcac tcggacgcgc gtgtcctgga tcagctcatc tataagtggt
    41041 cgcactcgcg tcgcatcatc gccgacgtgc tggccgacaa gtacatcgac ctggtccggt
    41101 cggggtggat gccgagcgag caggagatcc gacgggacgt cgccgatcta ttcggcggca
    41161 actttgagag gttctgcggt taaggattca ggagccgggg ttcagggtgc aggacccaga
    41221 cattgtcctg tgagagtatg tccgcaaccc cccaggagta ccggcgactc tactcgccca
    41281 cactacacgt tgccctgaaa cgtcaaccga tacatcctgg cgaaggcttt caggcgtttg
    41341 gcatcgccgc gattgccaca gaagaggatc ggatggggac cggcgtagac cttgcacacg
    41401 tcgtccacgt tgtccatttc catcagcacg cgcgtcgcgc agccgcctgt gggaggcgag
    41461 gtgggtgact cgatcacttt accggtccag cagttgaggg tgtcctgtcc gctgagatac
    41521 ttggccagaa tgatcggttc accgggcgtc cactgcacgt cgagggccgc cgtcttgggc
    41581 aactggtgga agaacggccg cacgaagtac tcctgcgaag gcccctgggg accgtgcagc
    41641 ttccaggcgc aagtgcagtg ggatgcaaag tatctgttct cgctcgtatc aaactcgggg
    41701 ttgtgctgaa agccgccgcg gcccagcagc catcggccca gcatcaacgt gagcgaggcg
    41761 ttgagatcgt tctcgcagcc gcacggaacg aggtttccgt tattcgtcgc gaagctgatg
    41821 cagggctttc gatgcttgag ttgcaggcac tcgatggtaa tggcgtcggc gccgtagcgg
    41881 tgcagcatct gctcgacggc ccgatgggcc ctcatggcat cgacgaaata cttgtcgacg
    41941 acgtcggtga ccctggcggc gttcttcttg aactgcatcg catcgcggac gagctcgtcg
    42001 tcatccgcga tgccgtcgaa gacggcgttg aattcctcgg cgggggcccc gacgacttcg
    42061 acgccgagcc gcggctcgga tatgatttca gcggcgggtt tggcggagat gcgcagcagc
    42121 cgactctgcg ccagcatctg cctggcatgg acggccgtca gagcccgctc tatttcatcc
    42181 cagttttcga tggaatggat gtagtagagc ccctctgcat ttcgcaggta atccggcgga
    42241 agctgatggt ttgaacccac cggatggtaa acgatcgccg tcggggcacc cggctcggtc
    42301 gccatctggt gcgaccattt ggaaaacgtg ttccagaagt tcacgatgaa gacggcatcc
    42361 ggtttggcgg ctttggcttg ttcgatgaac tctctgacct tggcctcctg atagatcgct
    42421 ttcggcatga actcgaccac cagcccgagc ttgtcggcgg tttccctgat cttcgcggta
    42481 aacttgcgtt cctgctgctc gggctcgaac tggttgcccg gccaggtgaa ccaattgtgt
    42541 acccgccacg agtcttccat cttcccttcg ttgacgacat ccgcgggcgg atacaaaaag
    42601 gcgaccagga ccctggccgg tttcttgtcg cgtttgggaa cggcagacgc cgcggccgca
    42661 ccaacctgcc gggccacggc ccgcgagacg aaagcggccg ccccgagcgc gccggtcgcc
    42721 gccaggaaac cacgacgatt caaaacatag gatgaacacg attcacacat gatctcgact
    42781 cctaaggcgg accgcctttt tgcgggccgc cacccaagtg cactgcgatc cagaaacatg
    42841 cgcgcaagcc gcacagatgt ccggccgtaa ggtcctattc tacggatgat gtcggcggca
    42901 tagtatatca gaccgcgggc cagagaccca gaaagaagcg gaggaatcac gggggactcc
    42961 atcaggcagc cgccaagaat cgcacacgat catcccggtc ttcgatcgag gacgcgcggg
    43021 attgcacccg aatccgaccg cagggacttt ggttcggcga aggtcacttt cgcgtccagg
    43081 ttcaagacgg tcttgatccg cctggccacc tgatttctca ggatttccag ggtcttgatc
    43141 tcgtcgagag acgggatgtc ttccaggacc tcgaccttaa tctcaagggt atcgacgtcg
    43201 ccttgtcggt ccaggatgat ctgatagtgt gggctgaggc cctcgacctc gtacaagatc
    43261 ttctcgatct ggacggggaa gaagcccacg ccgtggaaga agatgaggtc atccgtccgc
    43321 cgcgacacgc gggtcattct ggcgagggtt ctgccgcagg cgcacggctc gctattaacg
    43381 gaggtgatgt cgcccgtccg atagcggatc agcgggaagc cctccttggt gatggtggtc
    43441 agcaccaact ccccttcgca tcctggtccg agcggttcga gactggtggg gtcgatgacc
    43501 tcaacgatga agtggtcttc gttgatgtgc aacccgcacc gctggcggca ttcgccggcc
    43561 acaccgggcc ccatgatcgc ggtgaggcca taggtgtcga tcgcgataac gtggagcttt
    43621 tcttcgatcc gcttctgcag ctcgtcgctc caccgttcgc cgccgaagag ggccagccgc
    43681 agttggagcc gttcggggtg gactcccatc tcctcgagcc ccgcggcgat gttcagggcg
    43741 tggctcggcg tggtgatgag ggccgtggtt ttgaaatcct tcatgatggc gatctgtctg
    43801 gacatcgagg ccgtcatgga agccgggatg actgaggccc cgatgagttc ggcgccctgc
    43861 tggaatccgg gggcaccggg cagcaggccg tagtcgaggg cgatctggac cacgtcgtgc
    43921 tcggtgatcc cgccggcagt cagtacgcgg gcggcgcatt ccgtccagtt gcgcaggtcg
    43981 tttcgcgtgt agccgacgac gacgggcgtt ccggcgattg cgaacccctg gtggatccgc
    44041 acgatatcac gcagcggaac ggcgaacatg tcgtaggggt agcttcgcag gagatcgtcg
    44101 cgcgtcgtga agggcaggtc ccgcatggcc cgcaggtccc tgatcttctc aagattgacg
    44161 caattcgcgt cgaaggccgt tcgatagaag gcgacgctgc gatacacccg gttcagagtc
    44221 gactgcagcc gctcgatctg gagctgctcc aactcgtcgc ggggaagcgc ctcgcaagcg
    44281 gggttgtaaa gggcattccg gctcatgatg ttgcctcact catcgatacg ctcgtactcc
    44341 ttgcccaggt aagctcgctg gacctcgcga ttgtcgctca gctcccgcga agttccctcg
    44401 aggacgatct gcccggtttc gatgacgtat ccgcgatccg ccaccgccag ggccgcccgt
    44461 gcgttctgct cgaccagcag aatcgtcaag ccgtcgcgct tcaaggtcgt gatggtccgg
    44521 tagatgtcgc ggaccaccaa aggcgcgacc ccgagcgaag gctcatccat catcaacaat
    44581 cgcggcttgg acatcaaagc cctcgcgatg gcgagcatct gctgctgccc gccggacagc
    44641 gtgccggcaa gctgatgttc ccgctccttg aggatgtcaa agagccggca aacctcgtcc
    44701 atgcttgcct tgacggcctc gcgcgtttgt cccttgcccc ggcaggagta agccccgagg
    44761 atcaggtttt ccttcaccgt catggtgggg aagacatgac ggccctcggg gaccatcgag
    44821 cagccaagct gaacgattct atcaggcggc aggcctcgga ggtcctgctt gtcgaacagg
    44881 atcgttccgg atcgcggctg aaggataccg gcgatgctct taagcagcgt cgtcttgccc
    44941 gcgccgttgg ccccgatgat ggcgacgatc tccccctggg aaacgtgcag cgagattccc
    45001 ttgagaaccc gcaaggcgcc atagcctgcc tcaacattgg tcagtcgcag catggtcgtc
    45061 ccccaggtaa acgcgtatca cttcggggtc tctctgaatc acccgcggcg gtccttctgc
    45121 gatcttgtgg ccctgatcca ggaccacgac atcgtccgag atattcataa cgagcgacat
    45181 gtcgtgctcg accaccaggc aggtcacacc ccggccgcga atcttcaaga tcagatcggc
    45241 aagggcctgt gtctcataga tattgagccc ggccgccggc tcgtcaagaa gcaagagctg
    45301 cggctcggtg gccagggccc gcgcgaactc gaccagccgc tggcggccga aggacagatt
    45361 cgaggcgatc tccgaggcac acgccgacag gccgagatca tccaggatcg acatggcctt
    45421 gtctctgctc tccttctcct cgcgccaggt gcgcggcagg ttgagcatgc cggcgacgaa
    45481 accggaccgg gtcctcggat ggcggccgac catgacgttt tcaagaacgg tcatctgggg
    45541 aaacagcttc gtggtctgaa acgtcctggc gatccccagc ctcgcgatcg catgtgccct
    45601 ccagccggtg atccggctgc ctcgaaagta cacctcccct tcgtgagggg acaacgtgcc
    45661 ggcgatcaga ttgaaaagcg tcgttttgcc cgcgccgttg ggaccgatga tggccttgat
    45721 tgccccgcag ggcacgctga aactcacgtc ctcgacggca tgaagaccgc caaagaaacg
    45781 gtggagcctg tgcgtcgtca ggaggggctc agccatgaga cctcctggtc gacaagaatg
    45841 cgagagcgcg ccagcggggc accctgagca gtccttccgg cgcaaagatc atcatcgcga
    45901 taaggatgac gccgaaaacc gcgtcatcgt aggtgccgaa cacgccgcga agagacagga
    45961 agttcaggac cacgcccatc agcagcgctc cccacagatt tgccatccct ccgacagcca
    46021 cgatcgcgac gtagcggacg gacttcatga cgctgacttc cgaggggccg atgcttgcgt
    46081 tgtaatgcgt caggagggcg cccccggcgg cggcgaagag agcgctgagc acgaaggtgt
    46141 agagcttgta acggtaggta tcgattccca gcgaatgcgc cgcctcctcg ctctggtgga
    46201 tggatcgaag ggccctgccg atgcgcgagt gaaccaggtt gatggccagg accatggccg
    46261 cgaggacgac aacccacgcg atgtagtagt tctgcacgcg gaacgccgga gcgccgttga
    46321 cctgcagcgt atcgccgatc agtttgaaag gcgggacgtt tgaaatacca tcggcctggc
    46381 cgaagacctc cgcccccagg acgatgcggt agacgatgag gccgaaaccc agcgtggcca
    46441 tcgccaagta gtgtcctttc aaacggatga ccggcatgcc gatgatcagg gcgatcacag
    46501 cggtcacaag cagggccgct atgaaggccg gccaaggcag aatgctcagc atctgcctgc
    46561 cgtaaagatc ctgatgctca acggtcaggc cgagccaccg gcacaaccga accaggggat
    46621 gggtctgata ggccagcagg ttgcacgtcg tcagtaccgc cgagacatag ccgccgatgg
    46681 cgaagaaacc cgcgtggccc agagaggcct ggcctgcata tcccatcagc agacacaagc
    46741 cgatcaccac cagcgcatag tacgccgaca tggtgatctg ggtcatgtag aagggcttct
    46801 cagcggccga gagtccggtt tggatcagga ccaccagaag gatcgtgaca gcaacgggca
    46861 gatatctgcc gagacgcatc agtggtccct cagctcgatg gtctgccggc cggcaaacag
    46921 gccgctcggg cgcacaatca gcaccatgag caggacgacc agggcaaccg cctccttgta
    46981 ggcaacgggc atctggctca cggcaaaggc ttccatcagt ccgagcagca gtcccgccgc
    47041 aaccgcggcc atgctgttgc cgagccctcc gagaattgcc accgtgaagc ctttgatggc
    47101 caggggggcg cccatgtcgt actgcgtctg catcaccggg gaaatcgcgc atccggccag
    47161 cgcgccgatg gcggcgctga gcatgaaaga gagcgtgacc atgcgggaat ccttgatgcc
    47221 gcagagttgg gccgccatcc gatcgtcggc gcaggcccgc atcgcacgac cggtcggcgt
    47281 gaaccggaag aacagcgtca agccggcaac gatgacggca cacatgccca gcacccagag
    47341 gtcctggggg gagactcttg cgccgaaaag cgggaaggtc gagacggccg taccggtgaa
    47401 atacggaagg gccctgactt gctcgtccca cacgtgcagc atgccctcgc gcagcacaat
    47461 cgagatgccg atggtgatca cgatgagccg caggacgggg gcatggcgga cggggcggat
    47521 aaacacgaac tcgaccaacg cgcccagcag catggtgatg accaccgcca aggcgacggc
    47581 aaccggcagc ggcacgaaat ggttcaggct gatggccgtc atcgctccaa cgatgagaaa
    47641 ctcaccctgg gcgaagttga tgacgccggt ggtgctgtag atgatgttga agccgatggc
    47701 aacaattgcg tacacgctgc cgttacggat gccggcaacg caatactgca gcgcctgggt
    47761 gagttccatc taagctcaga ctcctgggat cggattgccg tgtatcgttt actcctttgg
    47821 ggcaatcaca aacttaccct ccttcacggt caggagttcg aaagcatcgg catccaagcc
    47881 ggtgtggtcc gtaggcgaca tattgaagat gcccgcggtt ccaacgagcc ctttgaggtt
    47941 ttcgatggca tcacggacct tctcgcgatc gtcggtacct gccttcttga tggcctcgac
    48001 gagaatcaac agggcgtcat aagcgtgccc accgaaggtg ctggcttcct cattgtattt
    48061 gctctcgtaa tccttcttgt aggacatcag cagggccttc tggggatggt cgtcgggcag
    48121 ttgctcggcg accaggaggc gtccgcacgg gaacagggtg ccgttagccg cctctccccc
    48181 ggcttgggcg tacttgatgt tgccgaaccc gtgactctgg aagaggggga cgtccaaacc
    48241 gatctgcttc atgttcttgg caaccaggct ctgggcggga acgatggacc agttgaccac
    48301 tgcctggacg tccttgccct tgaccttggt cagcacgtcg gtcaggtcag tcacctcctt
    48361 ggggtagacc tcggcgatgg caatagtcag accgtgctcc ggagccagtt tttcaagctg
    48421 ttccctgccg gccgtcccga agccatcact gctgaccacg acgccgattt tcgaaatgcc
    48481 gcgttttttc atggcctcat aaatcacctt cacggcatcg ctgtcttttt gagggctctt
    48541 aaatacgtag ctcgcgaggg gttcgacgat tttttccgcg gcggcgcacg agacaaggat
    48601 catcttgctg tcctggcaaa gctgcttgat agccatcgtc tcaccgctgg tcgacgggcc
    48661 gatgatcgcc agaaccttct cttcatcgat gagctgcttg gccagcgaga gggccttctc
    48721 ggggcttgcc ccggaatcct tgatgatgac ctccagcttg cggccgagga cgccgccctc
    48781 gttgttgatc ttctccacga gcatttgcgt cgtcttctct tcgggggccc ccaggaacgc
    48841 cgcgggaccg gtgatggaga agatggctcc gatcctgata ggaccggcgt cggccggttt
    48901 tgtggtcgtc ggggccttcg acgaatcctt gcacccggcc aagccaacga acaaccccag
    48961 acccacaatt cccagaatga tgcaagtgcg ctttgtcatg tctaccttcc tttcgaaacg
    49021 aagacagtca cagatccttt atcgcatctt ccgacaggat ggcgattccg cttcgcctga
    49081 gcgtctcggc tgcgcgagcc gcgtcgtcga tcttgaacac caccaatgcc tgctcggcct
    49141 ttttcttcat aaaggcgtaa atatactcga tgttgattcc ctcgcggtcc agcagctcaa
    49201 cgatccgatg gaggccgccc ggccggtcat cgatctcgat ggcaatgacc tcggtttcct
    49261 ggacgacgaa accattcaac ttgaggaccc gaacacaccg gacacggtca tccacgatca
    49321 gccggagcac cccgaagtcg ggcgtgtcgg ccagcgacaa ggctcggatg ttgatgcctt
    49381 ccccggccag caagccggtc acttcggcga ttctaccctt cttattctcc agaaagacgc
    49441 acacctgctt ggcgtacatg ctgatctcct tgacgcaagc caccgggtga aacgatgagt
    49501 gcgtgtatcc taccgccggc agcgagcagc gacaagccgc gccgagcgag tatggctgtc
    49561 gcgagtgcgg caaccgtcgt cgggcacgga cggacccggg agagacaatg gcgtaaacac
    49621 gccatgccgg cacggtggtc agcctcgcca ttgaagacgt ctgggtatcc ggcatcgcaa
    49681 tcagcggcga cggccgaacg gaggcgagac tgcgtgtcgg gaatctgaca aatgctaata
    49741 catgtctatg gagtttttta tatcttagac accagtatcg gggggtgggc gttcgaatgg
    49801 tcgtgtcact tcccttccgg agcggcaggg tcggcgtgct ccgaacatca aggcccctcg
    49861 cttgcggtcg gggttctgta agagccgcct ggactgtcac ggacccgccc ttcgccaacg
    49921 gccgggaacg gccttgatgt gttcggcccg gcaacatata ataaagcagg agttagacag
    49981 gaagcgtggt cttggatagg gaggtgtgtg gcctcggatg agtttgaata cgggctcgat
    50041 ggcgagtccg tgacaacctg attgacgcgc gtgcgtcaag acgctgcttc acagaaaacc
    50101 ggcaagttac ttatggtcaa ctttagccgt ttttctttgt gctcatgacc atgggtgctt
    50161 cacaaaacac
//
LOCUS       DAOTPW010000005        48333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_803_length_48333_cov_5.910353, whole genome shotgun sequence.
ACCESSION   DAOTPW010000005 DAOTPW010000000
VERSION     DAOTPW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 48333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48333
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_48333_cov_5.910353"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="P5316_00810"
     CDS             complement(<1..554)
                     /locus_tag="P5316_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR83521.1"
                     /translation="MSDAILHAVNVRKSYILGKVVLKVLKGVSLAVQRGEFLAIMGSS
                     GSGKSTLLHILGALDLPDEGTVVFENQDVFKVPNARRDRLRNVHFGFVFQFYHLLPEL
                     NVLENTLLPGMVGTSFLGWFSEGRRRRRYAAELLDRLGLGERIRHRPNELSGGERQRV
                     AIARALVNRPQVLLADKPTGNLDAA"
     gene            complement(547..2154)
                     /locus_tag="P5316_00815"
     CDS             complement(547..2154)
                     /locus_tag="P5316_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRR83522.1"
                     /translation="MYKFFLCLRYLRKRRIAFFAVAAVCLCVAMVLIVFSVMDGFLRM
                     VRDRSRGMLGDLVVENQSSLQGFYFYQEFIDELKADPQIGPHIQEATPVIYTYGVVRY
                     PDSQITKPAQVSGIRLDEYRKVNDFEKGLFYEKYYPGTTTLAPQRIPGCAPTAEGVYV
                     LPPDIEAAWQKWWASATPQERAKAPIDKRWPYNRVGQYWPVPLEELAASDRPGPMWYG
                     SELPGIILGTDMCATRDEKGNYERYHCRGEEVQVTLVPITESGQLRDATGPVRDIFRF
                     ADDCRTGVYDIDSMAAYVDFDRLQKLLNMNYREWTDEDTGQKVVNHARASQVQIKLKP
                     GADVLAVRNAIRARWDAFSGPRLALVKNPNLMVGVRVLTWEEKQRTFIEAVEKEKYLV
                     TILFGIISFVAVLLIGCVFYMIVQQKTRDIGIIKSVGATSLGVAGIFISYGAAVGVVG
                     GAVGTVIGVLFVHYINEVQDLLKWISPKAEVWNPQVYIFERIPNEVNPWHAAFIYMVA
                     IVVSMLGSLIAANKAARVWPVEALRYE"
     gene            complement(2215..3726)
                     /gene="lysS"
                     /locus_tag="P5316_00820"
     CDS             complement(2215..3726)
                     /gene="lysS"
                     /locus_tag="P5316_00820"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRR83523.1"
                     /translation="MSDLQQVEDRKAKRAKLREMGLDPYGWRYPGVQSVAEVLRVGES
                     LNLGPGRHAEGSSAKVAGRVVLHRPCGKLIFMTLRDGSGDIQVAVSKSAVDEQAFTVA
                     NKLLNLGDIIGLEGTLGRTKTGEMTVWARSLTVLCKAVQPPPAKWHGLKDIDLRYRRR
                     YVDLFANPAVRETFKKRSLIIDAIRRCLVDRGYFEVETPVLQPIYGGAAARPFITHHN
                     ALDMRLYLRISPELYLKRLLVGGMERVFEFSRVFRNEGVDSSHNPEFTLLELYQAYGD
                     YNDMMDITEAMIAAAIERIGGGYKRTYGDLELDFTLPWPRRRYADLLEEHAGVKINDI
                     DAVRRKASELGIEHKGMDDAIVINEVFEATVEPKLIQPTFVLDYPAPICPLTRRKPDD
                     PNTALRFEAFVAGTELGNAYTELNDPDVQEENFRRSLAGERRDETMAVLDEDFILALQ
                     YGMPPAGGLGVGIDRLVMLLTNSTSIRDVILFPLQRPKAATGTEDEEEAYEGA"
     gene            4110..6689
                     /locus_tag="P5316_00825"
     CDS             4110..6689
                     /locus_tag="P5316_00825"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRR83524.1"
                     /translation="MRPHGLTAFAMLLWWLGSPVMAQPRPFLFGVGRYYSTWAGRDRS
                     YDQAIWDHMVTIGVTISGTGLAWCDAEPVEGQYDWNIIDYTDFCVDEMLARGIEPTMF
                     VGLAPEWARLYPDLPPHQSPPAEEYVQQFMDFHRFVADRYKGRVKYYFFWNEPNGCSW
                     INPNCANSDSYPLYTRWLIRCSQAIKSEDPDAKIVAANLDYHSGVTHGYQYVQGMYNE
                     GAGPYFDVISIHPYDPSGTIHWQALIDTRSVMVANGDAHKPIWISEYGWDTADFLSTA
                     NKLTQVLTELKKPEWSFVEQANYLVLNGVAELKNYGLMSANLVPRPGYYAFRDFDKSF
                     PSFVDFAADVTSGVVPLAVQFTDRSYVPGASGWFWEFGDGQTSTLQNPQHTYTAVGTF
                     TVRLTVTGTGAPLSVQKDNYISVNPGEVDFSAAPAVGEAPLTVQFTDLTTLAEVSDWF
                     WEFGDGQTSTLQHPVHTYAVEGSFTVRLTVTTPGGQQSVEKRNWIRVGDFAKVAFLAE
                     AAPLNAADSQIVDYLRSLGLLVNVYDDERTNRPAAAEIAAEHDLVMASSTVLSANVAG
                     DFRHQSTPFIFWESSLARSDREAMGNGAYAASDQTQINVLDNTHPVTEGLPTGLVALV
                     DAPEMFSYCTQETASGVQVLAESASDPSHKTIIVAEPGAALLDGGTAAGRRIMLHLYD
                     TTWQQTNAMGRRVFTNAVAYCLGKPTAGFAASATSGVAPMEITFADQSAGAVTSWAWS
                     FGDGSTSCLRHPSHVYRVSGAYTVSLTVTGPGGPSTLTRTDYVLISDPPAADLDGDRD
                     VDQTDFGHLQACFSGPGANFTEGCGDADLDGDTDVDAEDFRRFRLCLSGPGVVADPAC
                     EQQ"
     gene            6721..9567
                     /locus_tag="P5316_00830"
     CDS             6721..9567
                     /locus_tag="P5316_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83525.1"
                     /translation="MNRLPGYLALILLLASASGASAVCTVTGDFTINCDGVTYRAVDD
                     ECVHYVDLAAHNAAHPGISLPDLKKRIIDNYDAAAVPAPVVVAMTDYVDCTQTDHNFT
                     DANIWPQSPYPNKPSRLMNISGKVFRVTAAPDAGFATYYYSYDVATGGQAGVPHLLVA
                     ELSNDQERYTSLQIHHPDAMAISPGLPWCPPYAGEPTYNPWGDPWWEKNYPKIEQGPA
                     FGPDVGLAVYTGRELPVDNQPFNISMIFHAKTATTRVAITSTGCNLVRTSTDGGAVSR
                     MWVFKFVDAMESRPPTLNLPADPAEQRRIGIYMTHPWYLYAHYGTPARLLGHRQEDLT
                     RMVRHLKHCGMNYLVFNAINGADRSVKSWYSGSACFNWNSAGDLLAELPPIAEAEGLE
                     LVPLITSLQDTNESLLSLPNEAYQLGSDGNQVRAFGLPTLDPLHPEVQTVMFSLLHEI
                     ASRCASSPAVRGIGIRVNGKIGTCYTSQQDSPRGARHSGYSAWDLQQFKNDTGSGVPT
                     SPPDAAYNWLVARPAEWESWINWRCTRTRQFWLACRDLIKTYRSDLIFYVQCDLPSEM
                     PGTNIEWPAETPYNLLRHHGYDPEMFAEDTGIIISRGVMVALDRFKVSTRWAAPHGTN
                     YQNYRLFHYAPGLAEFYRTAQGKACEFYQDYWEENANPYFEFGSPGSPVGFFRTNTPA
                     APGRPFFEGAVMAVRRQDPDTITWLGWNRPTLGHEADIRKFAQAFRALPAVKPLPFNG
                     TIEPSLPDQIAARWYRNRLAIINDSPNARTITLNFAGPIPGGQTLTDVVTGRMLMSAT
                     NPVRTTASFQAEAYSLNVFLYSLGEDADWDGVDGSHDNCPLNYNPDQADEDEDGFGDV
                     CDDCPHTVPGVVISSNGCPVPIRGDMDRDGDVDQIDFGLFQSCMTSVGVPSNDPACSG
                     AELDGDPGIGPPDLIILRNCISGPMIPASNDCIR"
     gene            complement(9654..12809)
                     /locus_tag="P5316_00835"
     CDS             complement(9654..12809)
                     /locus_tag="P5316_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRR83526.1"
                     /translation="MILSDVAIRNRTTVGVLTVLIAVFGIYAYISLPREAAPDVPIPF
                     ILVTTVYEGVSPQDIESTVTLKIEKELAGLKGVKEIRSSSAEGRSLITIEFMPNVKID
                     DAMQHVRDRLDRAKPELPRDAEEPTLTEINVAEFPILMVSISGDISPVALKLIADDLE
                     EALEAVPGVLNCDVLGGLEREIRLEIDQNRLSAYGLTIAEILSLVPAENVNISAGSLE
                     TPGTKFNVRVPAEFVEPEEVDKLVIAVRNGKPIYLTDVAGVRDTFKDRVSYARLWTPV
                     ADEAGRMHLVGSETITVTVQKRVGENILFIAEAVKHVIAEFQRRVPANVRFDITLDQS
                     KDIRLTIVDLENNLASGMILVVVVLMLAMGWRPSLIVAIEIPLSMLLSMVVLQLLGYT
                     LNMVVLYSLVMAVGMVVDNAIVIVENIYRHYTLTGNRVQAALIATAEVAWPVITSTFT
                     TIAAFAPMAFWPGIMGEFMKYLPITLIITLLSSLFVALVINPTLSSIFVKGRLKKGNQ
                     QSRFMRGYRRLLNVAVTHYLTTPSLAFLVLITLAMLYGKRAHGIELFPDADPRRAIIS
                     LRCPQGTNIRQTDSLTRLVEDRLQPFAAEFQRVITNVGSGGGDFLSGGMGSMGEHLAT
                     ITLEFRDYAVRERPSAQAIAEIRDALSDIAGTEIKVSKEEEGPPTGAAVTVRVIGEDF
                     KTLEDLSERARQLIADIPGLVNLRSDHEAARPELSFRVNRRRAMMLGVNTAIVGNYLK
                     TAIFGTEVGKYRQFNDEYDITVRLPRDQRENVDDLFRLRLPNNQGASVPLSSLGEFSY
                     TGGFGTISRVNQKRVITLTGDAEGRLGTEVLAEVQSRLAALAKDLPFGYEISYAGEKE
                     EQDKAQAFLTKAFVIALLLIALILVAQFNTLTVPLIIMTTVILSLVGVFAGLLVHAMP
                     FGIIMTGIGVISLAGIVVNNAIVLLDYTRQLRDRGMDLITASVEAGATRLRPVLLTAI
                     TTILGLIPMAAGVSYDFHTFSWSTRSESSEWWARMAVAVIYGLGFATLLTLVVVPCLY
                     VLSVRCTEGLARLWRGRREAS"
     gene            complement(12806..14158)
                     /locus_tag="P5316_00840"
     CDS             complement(12806..14158)
                     /locus_tag="P5316_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR83527.1"
                     /translation="MSTAIGPRQQTASGGRRRARLITGCLLLAGVAAVGIVASMPAPQ
                     TQAPPPQRVPVNVKTWRVEPVAEIADSFEITAVVQPYATVRVAAEVPGRIERYGTRST
                     EVRWQGSVLPVGSQIGEGQPVTVGDPLVILNRDLLQARFDRASAQYEYDEQEYERMLS
                     LYQRGNASSAEMRLARTRRDVSKADLDEAARSLARTTITAPISGILNDWPMDLGEYAV
                     PGDTVAEIVDIEKVKVCADVPERDVGYLRVGQTAEVLRSPRDENPLVGTITYINALAD
                     QVTRTTRIEVTVQNRISDSAESASFQGEYRLRSGQIVDVRLTRRILHNVIMAPLASVI
                     PLEEGKEVYVVVDGKAERRTVEMGFIRGRSVQISRGLEPGDLLIVEGHRLVSPGQPVN
                     VVVAPDAGGSENDSHPSPVAENPRPSDPQRHTAQATATRPSLPAPANRPSVPDVENML
                     "
     assembly_gap    14199..14208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14209..14884)
                     /locus_tag="P5316_00845"
     CDS             complement(<14209..14884)
                     /locus_tag="P5316_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRR83528.1"
                     /translation="MGTKERRERAKDATRQKILDAARELFAREGYEAVSVRRIADAIE
                     YSPTAIYVYFKDKADLMKELCHTDYEAFTRQAQQFADVANPIQRICLLARSYIRFAIE
                     HPNHFRLMFMTKPTPEMVQMDEEEMRQRGRGDPTRDGYAFLMECVQQAIAQGWVRDDL
                     QHDPELVAQVLWAGGHGVASLQVTRAEKEPWFNWKGADALGEATLRAVLRGILRDESI
                     LEGNDSA"
     gene            15142..15687
                     /locus_tag="P5316_00850"
     CDS             15142..15687
                     /locus_tag="P5316_00850"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRR83529.1"
                     /translation="MVNQTSTPCAAELLNLIREVPDYPKPGILFRDITPLLRDPAGLS
                     LSVELMTQPYRNRHIDIVVGAESRGFIFGTAVARNLSAGFVPIRKPGKLPCAKRGVTY
                     ALEYGTDTLEIHEDAIEKGQRVLMVDDLLATGGTMKACCELVEQLGGRIISVVFLIEL
                     AGLNGREKLRKWDVYSVLRYD"
     gene            15709..17145
                     /locus_tag="P5316_00855"
     CDS             15709..17145
                     /locus_tag="P5316_00855"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HRR83530.1"
                     /translation="METWYWTIFALLFGACTGSFLNVVIYRWPRGISLLSPSRSFCPT
                     CSQPIAWYDNLPVLSYLRLGGRCRSCRNPISLRYPFVELATMLVFVMTFDAFFAARQR
                     FGVGDMGDDWVILIAHWALWAGMIALAVMDLEAYMVDVNVTWSLLLIGLIGHVFWTPP
                     RSVEWIRPSPTLAAFALAVTVGLAVGALLFLRGPEEPSPEQSADEEAPAAPSVTSPRG
                     RIPIGLLVAVVFVVGYAVAVSLMGEQPRLRRPQLPDHSAVFSQSAEASQIDAGAIRLA
                     TGLALAFVGLVLAASQPSPEADQEIVDAIHEEAPKSRRNALLELRLLLPAFVLVGVLM
                     FVLASSQRTSFETHVRDVVHWSPAGHWKPIWGLSTALSGWVIGGAIGWFTRIVGTLLL
                     GKEALGMGDVHIMAAAGAVAGWPVVLIGFFLAAVLALLGLIVIHFRRQSRALPYGPWL
                     ALGFLVASVYQDRILLFLQVRWLLEQSL"
     gene            17400..18038
                     /locus_tag="P5316_00860"
     CDS             17400..18038
                     /locus_tag="P5316_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR83531.1"
                     /translation="MARLVSSLVRLFSLVLVISLFGCSDQLKQCQQDLTTANDRVQEL
                     QKLYDQAERARMAAEDEVTALKAERDRLKGELAKKPEGWDKVPGGALINIEGDILFDS
                     GKAELRKEGMPTLDKITDAIKQRYPNHEIYIFGHTDNDPIEKSNWKDNYELSCQRSLT
                     VLRYLKNAGVRNYLAACGWGETRPAQTNDTEEGKQANRRVQIFALDPKLRGQ"
     gene            complement(18208..18483)
                     /locus_tag="P5316_00865"
     CDS             complement(18208..18483)
                     /locus_tag="P5316_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83532.1"
                     /translation="MDFRVNLTIEVEQKANALAISKPVPVQLAYHRGQWHFECEDPSV
                     TVPACRSLEEALVMGSQQVAAEVQAAAVERPFVVGKITPDRIPLGMF"
     gene            18845..19774
                     /locus_tag="P5316_00870"
     CDS             18845..19774
                     /locus_tag="P5316_00870"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRR83533.1"
                     /translation="MDTTVLELIKRSAAIPSMPQVAVRFLEIIQDPDFDYREVVEVLS
                     SDPGTTCEILRLANSALFGVTRKVTALPHAMTLLGIKRIRSLVLGRYIVESLNRNGCA
                     SIEPAYYWRRSLTAAVLAARLADAILPDRREEAFISGLLADVGIVILDAAFPDKYRTI
                     AEQYRPGGNPDLALTEEILLGTTHGKVSATVLDHWKLPEAICEAVHWHSWECREQGRN
                     ALADLTGAANRISTWVCEAPTDLDAVVADCREIAHVLNLAPETLARCLMEIEEHVSDL
                     SSLLRIEVVSSQMYEVITTRISEGLTHLETVEV"
     gene            complement(19894..20763)
                     /locus_tag="P5316_00875"
     CDS             complement(19894..20763)
                     /locus_tag="P5316_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR83534.1"
                     /translation="MRLVVTADLHYDIARSMAPARRVAEQICGLDADALLVLGDVGGR
                     DPDIVRDGLHLFDRFKGRRFFVAGNHDIWTNPGEDSLDRLERVLPQICQDAGFHDLGL
                     APAEVDGVGLAGSIGWYDYSFRSEQLHIPLRFYEAKIAPGAASRLDEYAHLVADLSDV
                     PEEALRIGTRWMDGEHVRLPMSDVDFCRRLVERADAHLRQLERICRTIIFGIHHLPFE
                     ELVPHQDHPGWAFGSAFLGSTRFGELLLRCPKVRYVFCGHSHREHRMRIGHIDCVDVG
                     CTYISKRYEIVEV"
     gene            20928..21668
                     /locus_tag="P5316_00880"
     CDS             20928..21668
                     /locus_tag="P5316_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR83535.1"
                     /translation="MTDVLEIENLHKSFRLGSQVIRALNGVSLSVAQGTLAAIMGASG
                     SGKSTLLHMAGGLDLPDSGTVRIESKDLTRMSDRQRTLFRRKRIGIIFQAYNLLPTLT
                     AEENVAVPLLIDGLNSSQIRSRVAEMIRLVHLEHRAGHRPQAMSGGEQQRVAIARALL
                     NDPALILADEPTGNIDSISAVEVWELLRRLAHEMGKTVLMVTHEAAAASYADRIYVLK
                     DGQIIGRIEGIKPSDAALVASRYTELAR"
     gene            21625..24564
                     /locus_tag="P5316_00885"
     CDS             21625..24564
                     /locus_tag="P5316_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRR83536.1"
                     /translation="MRHWSQVGIRNWRVKPGRTAGAVGAIALGVGVVIWVTSAYESVR
                     LSLEDQVWLWIGRSHLSVESVFGQNGQVNQHLADKIAALPNVAHVTCCLQYYMYLQPL
                     IEDPETRKLVEGSGVSVMAMGIEPATEYHFRQYRRERPGNGGRLLTVDDADGLLVERQ
                     LAEQTGLAIGSKVLIRCRGPEWIKATEQSAVFTIVGVIEHRRVAKQQPPLIVGRLDRM
                     QELSQISKDVARVTRIDILLHDYSQKALRATQQQVRQIVDRDKQGFLVNSSEAKLAQV
                     EDAEQQTAFVMGLLSSVALLTGFFIILSTLSMGMVERIAQLGMLRCLGMTRTQTAMIV
                     LAEALPVALTGVLAGVPIGAGLAGLSVWLAPEYIGQWVISRPGMVLALAGGLITTLAG
                     AAIPAVQAMRVSPLAASRPQSQPAPGLVTWTAGAVGLAMMAGYFYAILWTPLIQHLRH
                     PAYPLVLISLLYGGCALIMPAAIRLVGQAAVMAAAAVLRVQWRLLSDQVGRAAWRSAA
                     ICCGLMVGLSLIVSLVVFGKSIAGGWDFPSQFAEAFVFISPPVDRQKARRQVQDFKGV
                     KSSCLVNEGLRCTVFGRSLFYFPWCTFVAGDPDEFFRIAKLEFVQGNQQDAEAKLRKG
                     GYILVTPEFTRTYMVDVGDTVNIRMNEAKRRMHRFEIGGVVTSPALQIAANYFNASGM
                     LTQHSAFVVMGTFEDARRLFEVPDVASMFLINFDLPQTSPPAEFAEEMPPRVSDAGAL
                     ADMLETWRASLPERSEELDRIVEQCRPLRTAGMPIHWLQVPLLRVFQESLLSGPNPAW
                     ADLTPEQRWRTFREELVMQLLAAKVGSSSPLHASARALKDQIDRDIQRAMLLFASVPT
                     VALFIAALGVANLMMANVTSRTRQIAMLRAVGATKWQVGRLVIGEALVLGLLGSLGGV
                     AVGLLAAHGVNKIVYKVWGFALETTVPWHWVGLGVAFTAAICLIAGLLPARYASRNNI
                     IDAMQTT"
     gene            24601..24786
                     /locus_tag="P5316_00890"
     CDS             24601..24786
                     /locus_tag="P5316_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83537.1"
                     /translation="MNMTVHAVSFASATLFFAGLAAGTEKTIVRFRLMDTAAGQTTPA
                     MVCVAGASDGEARNRRR"
     gene            24963..25544
                     /locus_tag="P5316_00895"
     CDS             24963..25544
                     /locus_tag="P5316_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83538.1"
                     /translation="MTGKRKRPIALLMVLGVIAATFYFCFIDRAWRHTERHGWVLDFS
                     YVRLACPTCLGRIERLEYKGQPIKAPADSHLRISLLTPVVSVESWDEQGRWRYHGDER
                     VAMVTGEPLSEETISEGFYTVDLSIRGGFPRRRPGTPESWCMLCRWDIAKGYWLAPEK
                     YQQLLQMLLNDGPQETRQEQAERSTTGSAPAEN"
     gene            25771..28683
                     /locus_tag="P5316_00900"
     CDS             25771..28683
                     /locus_tag="P5316_00900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HRR83539.1"
                     /translation="MTRIRIWTCAAILLGSTPAFSEIERWYMNEEWYDWHAASEFAVD
                     KIGLVSVEVHFVGPGAVTPRGLYIRGDADQPLERIEVLGFKDHRKEPWKYEPAVIKAE
                     GTPTGKKWVRLATGANPRGFELLTLKLPLGSDSSVPKFDYVCVSPNERYIAHGRAERW
                     IRDREKGKEVRCGMPLGGIGAGKIEIARDGSFRNITTNNNIDTPFYHPDLCFMAASVN
                     GHARILRDEPAVGLTPVEKIDFNARYPIAQMTFTDKAWPIPIALTAWSPIIPGNINDS
                     SLPAAVLEFKLENTTKHRQYVAVAVSWENLIGSGGRPQPGSLWGKTGHYLRFREDEGN
                     TTTSFSGGMDGPPAGFIFSGGPKTEKDSQGNHALMVWLPDFMGEFGPGWTLMPYNAET
                     GPREWKDFGRLPPHDLRNVSPQPHTAGGVSIRGELFPGETVVVPFILTWYMDHFHQLG
                     KEDLGHYYHNRFKNVREVAEYVLKNRERLFGETKALHDLFDRSNLPAWFNDMLLNDLY
                     VLSTDTWLTKDGRFSVNEGATSMFGCMGTMDQKLYASHHLALLFPQLQKQELRQFGQL
                     QNDNGGITHDLGTADFAAKLKAFDWPDLCSAFSILSYQVYRYTGDKTFWEEMRPKVLK
                     AIDCLAGDWDPDRLGVPGRGSTFDDEDSYRIFSYTTGLYLCTLKLAMTIAREMGDDKL
                     LATYQARYDRARDLAMKELWTGNYFRYGSSPPPENKRTDASHFSQMAGEFWCRLLGFD
                     GIFDEQVRQTALSNTLALHWNENFKLPPKIVTPDGRLFPRDGEHRNAPVSWPMHSRAL
                     LCGSAFLFDMAPTGWDLIKAMRDNIIAANGPDPWDQSLYYDPITARIDWGVFYMTAPA
                     SWLAYQALADTHYDAVSGTLTLRPRALAKVCPGKWPIITPTFWAIGEVSADGKKLSVD
                     VTKTFADGMAVNKILADGEIEQVWVTSSEPGKQIEKVSMRDLMPLRAGLRCQITLK"
     gene            28728..30158
                     /locus_tag="P5316_00905"
     CDS             28728..30158
                     /locus_tag="P5316_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83540.1"
                     /translation="MKKNISRRTVLARAGAAAVGAAWARTLVGQSTRPVGSSLGAGER
                     VGVGLIGCGQRGWQLLDLLLRQEDVDIPAVCDVDSERVGLAAQRVEKAGRRRPDAVAD
                     FRKMLDRSDVHAVVIATPDHWHAVQHVYACTAGKDVYVESPVGHNVLEGAAMARITRK
                     HFRVVQVGMQHRSAAWFRDAVQQVRAGTLGKIAQTRSWNFAKLKPVPPSDDAQPPASL
                     DYDKWLGPAPVRPYNPGRTHGLFYNWWEYGGGMLTRWNVHYQDLIHWAMRVNGPVSVT
                     AVGGNQGLGDFRETPDTMEVIFEYDTQLLPRPGRFTHVYSLRLGNGHAVWGPPVPAHN
                     DMFDAISSMHSGALFHGEEATLLVDRRGSAVLPAALRQDKPIDTAPVENANPPFDALT
                     LAHLQDFVSCVRTRAEPKASIEVGQYAAAACHLANIAYRTGRRIYSKPGELACFADST
                     YETVDTQATEMLRRTAYREPYGPPRT"
     gene            30216..33566
                     /locus_tag="P5316_00910"
     CDS             30216..33566
                     /locus_tag="P5316_00910"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HRR83541.1"
                     /translation="MIRRGKRLNLAATCGLTLVLSTGAAHGDVRVWEEQVVVPTYRVG
                     APDPNPIFYSKESYQGAQKRVYPYALQDRLTHVREERSYTALNLENQYVKLTVLPEIG
                     GRLFAATDKTNGYDFFYRQHVIKPAFIGMLGAWISGGVEWCAFHHHRNTTFMPVDYCL
                     AEDPDGSKTIWLGETERRHRMRWLVGLTLHPGRSYVEATVKLFNRTPQPHSILYWANV
                     AVHVNEDYQIIFPPSVQVVTHHSKIDFTTWPIAQGRYLGRDYTGVDLSWWKNMPNPNS
                     FFAWNLQEDFMGGYDHGRQAGVVHVANHHLVCGAKVWEWGKGEAARLWDSILTDNDGP
                     YVELMVGAWSDNQPDYSWIKPYETKVIKQYWYPVRQIGGFKNANLNGAVNLELKPDNQ
                     LAFGFHATTKHDNAKVILSAAGTTLFEQTVEIGPEKPFAREIAVPAGVSRADLRAVLI
                     SADGRELIAYQPVQREPVKELPAVVKAPPGPQEIKNIEELYLTGLRIEQIHNPGVNPD
                     DYYREALKRDPGDTRCNTILGVRANKRGLYEEAEKHLRRAIERLSAAYTRPGDTEAYY
                     QLGLSLRAQGRCDEAYDNFYRASWDQAFHSAAFNQLAELSCREGAFVEAREQIERSLS
                     TNTLNNKARSLQSLVLRKLGDYQAAKASSSAALANDPLDFFARNELYLALAAEKAPNA
                     AADVLVELRRLMRDEVQSYLELATDYMNCSQWNEAIEVLRRPIETKMPFAATYPLVHY
                     YLGYLYEQKGNPAQAHESYTRAAAMPSDYCFPFRLETADVLNAAIRANPSDARAHYHL
                     GNLLFDLQPEAAIACWEKSAAIDDQFAMVHRNLGWACHRVLNDVGRAIECYEKAVTRN
                     PMDPRLFFELDGLYETANVDPARRLAVLEKNHEVVRQHQDSLLREIMVLVLVGRYEQA
                     IDHLSKSQFHAREDTGHVYETYVDAHLLRGLTLLRVGRNVEALADFQRAAESPKNLAT
                     PRNNLRVPEIAYHTAMARKALGETAKAGEAFEEGASHPGSASIPQTRYCHALCLAELG
                     KADEAAKVFDDLIRTGKEKLTTRTDEPDFFAKFGEQEATNARAASAHLSIGLGLLGKG
                     KPSEAKQEFEQAVRLNLGNVWAAYYLRP"
     gene            complement(33606..36227)
                     /locus_tag="P5316_00915"
     CDS             complement(33606..36227)
                     /locus_tag="P5316_00915"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRR83542.1"
                     /translation="MLRTCLFCQSLILLGLLSAPAEAVAPPEWAVMECRGLKAWTEVI
                     YYWIDRQAEDGSFGFGLNDDCEFYEMWPIVVMAADDQRIIESLRKALDWVWYNESLMD
                     GYESGARDVAHAAEVTSNTQPLMTIIDYGNPVFIERMMTTSKNVEKWTAVNARGHRHF
                     RSNFFGSQEIWEHAYFGSDAGICARAMIPAMHLLWYNRDPHLARYCLEWGRAWVDHAK
                     ETAYGKPYGMLPCEVVFATDEAGGFTRTWYTGAVVLHNQRIRLLELLILDYAISGDPD
                     FLLPARTELGMYGMLRHGYAIKDILGPENRSNADSVTLPPDEKTRYGRTGAELLYTYR
                     WATGDHEYDRWWGSPPRMTRQIALEAGRKAVEYARVEAEKARRINIRNGTDNYEASID
                     LLGEHFPLLYYGTDHSSPDFVDLPFPPVRWLRGDYELAVVMLEHSLTRFKALCCNVGE
                     KPRSIGAQLFQLEPGAYRVTFGIDANADDRPDRLVRDETVIIERGSILDFDLEPGYEY
                     IMEFIQLAEGPAWESRADVAVCSDDLFCVPAAPDPGTEAELRIRVHNIGTEDARQVKV
                     LAEELGSGRVIGEQLIETLACPRALVPSSVMVSMPWRVGPKATGVRVRVDTGNLVKEI
                     YEGNNAAERRLADLPEGPRPLRPIYLPGWYRDQQKGPVPELTAVYVEGITVDGRLDEP
                     GWQKAERIGPLRTNHNEPSEKPTYVRLAYGSDAFYVAIEATEPRMDLLVANARGPDSA
                     GIFGDDSFEVFIDSRFDKMDYYQFAANTAGATIEGRFYNFTLYNEPWECKVQKGKDLW
                     VAEVRIPFKSVNAQPQPGSKWGVNVYRTAMTFTMPQSLEDREKGWKHTEQIALSPTFT
                     GYHEVGRFAAVTFGPKQ"
     gene            36635..38389
                     /locus_tag="P5316_00920"
     CDS             36635..38389
                     /locus_tag="P5316_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR83543.1"
                     /translation="MIGRTVSMFVVVCACVILFHAVSGCGTSQGASSSSFTFTVTADI
                     EGAAFTAPDELRLLFRDIKDTGGPGAFMIHVGDHMPGIEAQDIELKKAFGADFPWYCV
                     LGNNDQKEPEKSMRFIRSHLRELPYIVNVGPFNSETTTYSFDYGDAHFVVLNLYYDGC
                     TDHGTRRPGTAGKVIPQLYYWLADDLAATDKKWIFVAAHEPAFPQPDADWGDVRHEND
                     SLNEYPAQRDALWELLSDHRVVAYFNGHIHRYARYLKDGVWQVSVAQSRGNSKYDSYV
                     RVHVGRSEVTFDVYRPLHEGVFRKTDTWEVTRPDGPTAFPHAMADPDAAWVGIEYVKQ
                     LSLTQRCPATDWSVIEGPPGLTVDRHGFVRGWTPTAQHVGSDRSITVRAANTRGVAEV
                     TWPVRVSPLPADTVAMFPFNTGPEGWRLETWMGGKYGPASISWQVLGGHPGGTVFSVG
                     HGANDDNRCTREGTILTRAISTRGFRDIRVEFDVIGDLAAPPCGQTDGVEGGVDGSSE
                     DQLGVCYSTTGPEGPWTTARVFKAGDLPAEWTRKVVDLSTAQGTADNPDFALRLVWQF
                     NTFSDSGRIDNVAVLGAR"
     gene            complement(38412..40208)
                     /locus_tag="P5316_00925"
     CDS             complement(38412..40208)
                     /locus_tag="P5316_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR83544.1"
                     /translation="MEIPRRARGDELLKPGQMTGLRESLRRISGKHDLASVIACAFDH
                     RTRMLPFIYADTRMAPAGVRAIGSAMADVGFSKTRIVLQQWNPNFKPSLMRLDGRVPD
                     LFMVSSMQIHTAQCHALIRDACRIDPSKRPLIIAGGPKVIYEPWDVFSSDPRQPWGAD
                     VAVTGEEYVLLSLMEVLLSVRAEGEPLRSAFRRARDGGMLDGIPGLVYSLGEKDGVAE
                     ELVDTGTQRLLSDLDELPHPVLGYRLLEPPSREATLAAESLQAGRVRKYSPLASLVLT
                     FGCKFNCPYCPIPAYNQRQHRAKSGARIADEFIRLHQEYGIRYFFGTDDNFFNNKQRT
                     LGIVETLARSAINGGKFRKTVRWATEVTVHDTLQMRDHLSLVRAGGIRALWLGVEDMT
                     GALIRKGQSVDGTEEVFRLLSRHGIVPMPMMMHHDGQPLYTRGSPCGLLNQVRLLRRA
                     GAAGLQVLMITPATGSRGYEESFTSGLTYAIAAGRQVEQHMLDGNYVVASRDPRPWRK
                     QLNIMLAYLYFYNPLRFLRAIIRPRTRLYLADAMMQVLGMWGTIQTVRRTLGWALCLK
                     RGPIRRHTRPPQPAVPVRKAAVSTSVLAQADA"
     gene            complement(40493..41365)
                     /locus_tag="P5316_00930"
     CDS             complement(40493..41365)
                     /locus_tag="P5316_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83545.1"
                     /translation="MSKPSGEYRPDRRARKWRLLSLFSGLLLAACFLLPAVQGCNGPI
                     VPAASLVWELSDAWNGSWLIDVVAVVVVYFVAYAFGAALMIGALAALVAWPKLRSAST
                     LAVIILLAYAAVVVLASTLRQGFTNGWAPSSWTTQDYVNLVFGLLCSPLLLAGTIFAI
                     RRRHQRRLCLWFLGGLSGLAWFSYWPIFSNPLYGLYLSLAACLLIVLASVGEAAVLTD
                     QSWLRTFGQLLTCRLAPFYDPKGRCPGCDYYLYGLTEQRCPECGRPFTFDELNATPEE
                     LSFASTPLCRNRDG"
     gene            41787..43889
                     /locus_tag="P5316_00935"
     CDS             41787..43889
                     /locus_tag="P5316_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR83546.1"
                     /translation="MIKSMLRVIVCGAFAACVAAILGADTGPEPKPPVRTNKLRVLIV
                     TGGHDFQRKEFFEMFDGHPDVAWEEVQQPEAQKWFAPDKADSYDVMVWYDMYSQITEE
                     SRKNLEALLKKGKPLVAMHHAAGDYQDWPEAIKIIGAKFNLKPGPTERKPSFKHDVKV
                     RVKIADPQHPITRFMKDFEIEDETYKDMEFLEGTHPLLTTDEPTSDKVIAWTHTYHKS
                     PVVYIQLGHDAKAYTNPNLRRLVVQSIRFVAGRLPDPSTEGFVQLLNGTDFTGWTIVG
                     DPKGFWFTDGVLRSESGKGGLWMRTNKIYRDFILRVEWRIGKDGNSGVFVRAAAEGYP
                     WETGSEIQISNEPRDIAHCTGSLYGTVAVDPRPDETADVWHMFEIHCRGYHYKVFADN
                     IPVVDVDAKTIPALAAKPLEGYIGVQDSHNRTSYVEYRGIWVKELTPMSSDKEPAWRV
                     GTQAYTFNRFTFFEAVDKASSIGLKYIEAYPGQRLSPGLGSAKFDHDMAPDLRQTTKT
                     KLAEAGIRLVNYGVVGLGNDEAAARKVFEFAKDMGIETIVSEPEPGSFQMLDKLTQEY
                     EINVAIHNHPKPSRYWNPDTVLDAIKGTNSRIGACADTGHWLRSGLDPIECLKKLEGR
                     IICLHLKDLNEKAMTAHDVHWGEGVANMKGILAELKRQGFKGVFSAEYEHNWENNAPD
                     VAACVQNFEKMAKELGMN"
     gene            complement(44688..44876)
                     /locus_tag="P5316_00940"
     CDS             complement(44688..44876)
                     /locus_tag="P5316_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83547.1"
                     /translation="MTISVAKRNKQALPAVLKLTMQTYTDPQLLDTFGAVERLPELRP
                     ENQPKPNRKTGLTTMEAL"
     gene            45808..46539
                     /locus_tag="P5316_00945"
     CDS             45808..46539
                     /locus_tag="P5316_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRR83548.1"
                     /translation="MACDGYLPLWNRVLLDVCTQRDFLTPGCVLQVANLDSLIANLQR
                     VFGWVRSNGIAVVSLVESHRPTEPMREFPLHCIDNTPGQEKLPFTLLSPRTLVETDNY
                     LSLPPDLQANNRQLIFRKRTRDILSNPKADRFLTSWQPEEYIIVGVGLERAIRGLALG
                     LLARHKTVTVVTDACGCWSPADAELVIRQLGAKDIRLTTVEELTRPPVVVAKRTLLRL
                     RRSRRHHPVGSRPAKLSSRAKVANG"
     gene            46836..>48333
                     /gene="nifA"
                     /locus_tag="P5316_00950"
     CDS             46836..>48333
                     /gene="nifA"
                     /locus_tag="P5316_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HRR83549.1"
                     /translation="MSSLDDPSQGLPKTKQLPVKREVKELSLLFEISQMLDRSLDLRE
                     VVHPILQLLHERMGMSRGTLTLLDRETGQAHIEAAHGLSASQCRRGMYAPGEGITGKV
                     IESGRLMVVPRVSEEPRFLDRTQSRKHLRKQDISFICVPIKLGNEVIGALSVDRLFSE
                     EVRLDEDVRLLSIIASMIAQAVRLRQSAQAERQRLIEDNLRLQDELKERFRPANIIGN
                     SRVMQAVYDQIARVSKSDATVLICGESGTGKELVANAIHYNSPRAGKPFVKVNCAALP
                     ETILESELFGHEKGAFTGAVAQRVGRFEMASGGTIFLDEIGDFSMATQVKLLRFLQER
                     EFERVGGTKTIRVDVRVITATNRNLEQLIAENKFRQDLYYRLNVFPIHLPPLRARKSD
                     ILLLADHFVLKYGSLNHKKIKRISTPAIDMLMAYHWPGNVRELENCIERAVLVSDDEV
                     IHGHHLPPSLQTAEASHTNLVGTLQVALDNLERELIVDALKSSRGNAAK"
BASE COUNT         9564 a        14894 c        14659 g         9206 t
ORIGIN      
        1 gccgcgtcca gattacccgt cggcttgtcg gcaagcaaga cttgcggacg gttgaccagg
       61 gcgcgggcga tggcgacccg ctggcgttct ccgccggaca gctcgttcgg ccggtgtctg
      121 atgcgttcgc cgagacccag acgatcgagc agctcggccg cgtatcgccg gcgccgacgc
      181 ccttcggaga accagccaag aaacgaggtg cccaccatgc cgggcaaaag ggtgttttcc
      241 aggacattca gctccgggag caggtggtag aactggaaaa caaagccgaa atgcacgttt
      301 ctcagccggt ctcgccgggc gtttggcact ttgaatacgt cctggttctc aaagaccacc
      361 gtgccttcgt cggggagatc cagcgccccg aggatatgca gcagggtact cttgccgctg
      421 ccggaactgc ccatgatcgc caggaactcg ccgcgctgca ccgccagcga cacccctttg
      481 agcaccttga gcacaacctt gcccaggatg tacgacttgc gaacgttgac cgcatggagg
      541 atggcgtcac tcatatcgca aagcctccac cggccagacc cgcgccgcct tgttcgcggc
      601 gatcagcgac ccaagcatcg agacgacaat ggcgaccatg tagataaacg cggcatgcca
      661 gggattcacc tcattcggta tgcgttcgaa gatgtagacc tgcgggttcc agacctcggc
      721 cttcggactg atccatttga gcagatcctg aacctcgttg atgtagtgga cgaacaagac
      781 gccgatgacc gtgcctactg cgccgccgac cacgcctacg gccgccccgt atgagatgaa
      841 aatgcccgcg acgccgagcg acgtggcccc gacgctcttg atgatgccga tatcgcgggt
      901 cttctgctgc acgatcatgt agaagacgca gccgatcagc aaaaccgcca cgaaactgat
      961 gatgccgaac aggatggtga ccaggtactt ctctttctcc acagcctcaa tgaacgtccg
     1021 ctgcttctcc tcccaggtaa gcacccggac gccgaccatc aggttcgggt ttttcaccaa
     1081 agccagccgc ggtccggaaa acgcgtccca tcgggcccga atggcgtttc tgaccgccaa
     1141 tacatccgcg cctggtttga gcttgatctg cacctgggac gctcgcgcgt ggttgacgac
     1201 cttctgcccg gtgtcttcat ccgtccattc gcggtagttc atgttgagca gcttttgaag
     1261 gcggtcgaaa tcaacgtagg cggccattga atcgatatca taaacgcccg tacggcagtc
     1321 atccgcgaag cggaagatgt ctctgaccgg acccgtggcg tctctgagct ggccgctctc
     1381 ggtgatcggt acgagtgtga cctgtacctc ctcgccgcga caatggtacc gctcatagtt
     1441 tcccttctca tcgcgcgtag cgcacatgtc cgtgccgagg atgatgcccg gcagctccga
     1501 gccgtaccac atggggccgg gccggtcgga tgccgccagc tcctcaaggg gcaccggcca
     1561 gtattggccg acgcggttgt aaggccacct cttgtcgatg ggagctttgg cccgctcttg
     1621 cggcgtggct gaagcccacc atttctgcca ggcggcctcg atgtcaggcg ggagcacgta
     1681 gacgccctcg gccgtcgggg cgcaaccggg aatccgctgc ggcgccagcg tcgtggtgcc
     1741 tggatagtac ttttcgtaga aaaggccctt ctcaaaatcg ttgaccttgc gatactcatc
     1801 aagccggatc ccgctgacct gcgccggctt ggtaatctgt gagtccgggt accgtaccac
     1861 gccgtaagtg tatatgaccg gcgtggcctc ctggatgtgc gggccgatct gcggatcggc
     1921 tttcagttcg tcgatgaact cctgatagaa gtagaagccc tgaagcgagg actggttctc
     1981 gacgaccagg tcgccgagca tgcctcgcga gcggtcgcgg accatccgca gaaagccgtc
     2041 catgacgctg aaaacaatca gcaccatggc cacgcacagg cagacggccg ccaccgcaaa
     2101 gaaagcgatt cggcgcttgc gaaggtatcg aaggcagagg aaaaacttat acatcgagtc
     2161 aatcagttcc gatcgcggct gcgagttgcc tgccgggctc acgcgtcccc ggcctcaagc
     2221 tccttcgtaa gcctcttcct cgtcctcggt accggtcgcc gccttgggcc gctgcagcgg
     2281 gaacaggatg acgtcacgaa tgctcgtcga gttcgtcagc agcatcacca gccggtcgat
     2341 ccccacgccc agcccccctg ccggcggcat gccgtattgc agggccagga tgaagtcctc
     2401 atcgagcacc gccatggtct cgtcgcggcg ttcgccggcc aggctgcggc ggaaattctc
     2461 ctcctggacg tccggatcgt tcagctcggt gtaggcgttg cccagctccg tcccagccac
     2521 gaacgcctcg aaacgcaggg cggtgttcgg gtcatcaggc ttacgtcggg tcagagggca
     2581 gatcggcgcc gggtagtcca gcacaaatgt cggctgaatc agcttcggtt caacagtcgc
     2641 ctcaaagacc tcgttgatga cgatggcgtc gtccatgccc ttgtgctcga tgcccagttc
     2701 ggacgccttc cgccggacag cgtcgatatc gttgatcttt acacctgcgt gctcttcgag
     2761 caggtcggcg taccttcgtc ttggccacgg caacgtaaag tcgagttcca agtctccgta
     2821 tgtacgctta tacccaccgc cgatgcgttc gatcgcagcc gcgatcatgg cctcggtaat
     2881 atccatcata tcgttgtagt cgccatacgc ctggtaaagt tccagcaggg tgaattcggg
     2941 attgtgcgag ctgtccacgc cctcattgcg aaagactcga gagaactcga atacccgctc
     3001 catcccgccg accagcagcc gcttgaggta aagctccggg ctgatgcgca agtagagtcg
     3061 catatcgagg gcgttgtggt gggtgatgaa cggccgggcc gccgccccgc cgtagatcgg
     3121 ttgaagcaca ggggtttcga cctcgaagta gccgcggtct accaggcagc gacgaatcgc
     3181 gtcgatgatc aggctccgct tcttgaacgt ctcccggaca gccggattgg cgaacaggtc
     3241 gacgtatcgg cggcggtacc gcaggtcaat gtccttcagg ccgtgccact tggcgggcgg
     3301 gggctggacg gccttgcaca aaaccgtgag cgatctggcc cagacggtca tctcgccggt
     3361 cttggttcta cccagagttc cttccagacc gatgatgtcg ccgagattca gcagcttgtt
     3421 cgcgacggtg aaggcttgtt catccaccgc tgacttgctg accgccacct ggatgtctcc
     3481 cgagccgtcg cgaagcgtca tgaagatgag cttgccgcac ggccggtgaa gtacgactcg
     3541 gccggcaacc ttggccgagc tgccctcggc atgccggcct ggaccgagat tgagggactc
     3601 gccgacccgc aacacctccg cgacagactg gacgcctggg taccgccacc cgtaagggtc
     3661 caaacccatt tctcgcagct tggcccgctt ggctttacga tcttctacct gttggaggtc
     3721 gctcattgtc ttccgatcaa ccttcgttcc gcgcctgtcc ctctgccaat ggggacccaa
     3781 cgaagtcggt cacggcgata ctaccggctt gcgactgttg gcggtgggcc accagtctca
     3841 gctgcccggg tgccatggcc tttgcctgac ctaccggccc ggcggctcaa cagacgaatg
     3901 aacccacaac aggccaagac acgtcttcgc tgatggtatt cggcgggggt agcggatgca
     3961 aatcggccaa tgcgatacga ggacggtcag acagaccgac acgatagttc ccccagctgc
     4021 ggcggacgaa aacaggtttt cctctcgacg caaccatgct acaatagaaa ggagtcgcag
     4081 agcagcatgc tttccgggaa gccccgatta tgagacctca tggactgacc gccttcgcta
     4141 tgctgttgtg gtggcttggt tcgcccgtga tggcccagcc acggcctttc ctgttcggcg
     4201 tgggtcgcta ctattcgact tgggcaggca gagaccgatc ctacgaccag gccatctggg
     4261 atcacatggt caccatcggg gtcacgatca gcggcaccgg tctggcttgg tgcgacgctg
     4321 aacccgtgga aggccagtac gattggaaca tcatcgacta cacggatttt tgcgtcgatg
     4381 aaatgctggc tcgcgggatt gaaccgacca tgtttgtggg gctggccccg gagtgggcca
     4441 ggctctatcc ggatcttccc ccgcaccagt cgccgccggc cgaggagtat gtccagcagt
     4501 tcatggactt tcaccgcttc gtggctgatc ggtacaaggg ccgggtgaag tactacttct
     4561 tctggaacga gcccaacggt tgcagttgga tcaacccgaa ctgtgccaac tccgatagct
     4621 acccgcttta cacgcggtgg ctgatccgct gcagccaggc tatcaagagc gaagatcccg
     4681 atgcgaagat cgtcgcagcc aaccttgatt atcactccgg cgtcacccac ggctaccagt
     4741 acgtccaggg gatgtacaac gagggcgcgg gaccgtattt cgacgtcatc tcgatccatc
     4801 cgtacgaccc gagcggcacg atccactggc aggctctcat cgacactcgc agcgtgatgg
     4861 tggccaacgg cgatgcccac aagcctatct ggatcagtga gtatggctgg gacacggccg
     4921 acttcctctc gaccgccaac aagctgacac aagtgttgac ggaactgaag aagcccgagt
     4981 ggtcgttcgt ggaacaggcg aactatctcg tacttaacgg cgttgccgag ctcaagaatt
     5041 acgggctgat gagcgccaat ctggttccac ggcccggata ctatgcgttc cgcgatttcg
     5101 ataagtcctt tccctctttc gtcgacttcg ccgcagatgt gacctcgggc gtggtgccgc
     5161 tggccgtgca gtttacagac cgatcttacg ttcctggcgc ctcgggctgg ttctgggaat
     5221 tcggcgacgg gcagaccagc acactccaga accctcagca cacgtatacc gccgtcggca
     5281 ccttcaccgt gcggctgacg gtgaccggta ccggcgcacc gctcagcgtt cagaaggaca
     5341 actacatcag cgtcaacccc ggtgaggtcg acttctccgc cgctccggcc gtcggcgagg
     5401 ccccgctgac cgtccagttc accgatctga caaccttagc agaggtttct gactggttct
     5461 gggagttcgg cgacggccaa accagcacgc tgcagcatcc cgttcacact tacgcggtcg
     5521 aggggtcctt caccgtccga cttacggtca ccacgcccgg cgggcaacag agcgtcgaga
     5581 aacggaactg gatccgcgtc ggggacttcg ccaaggtcgc gttcctcgca gaggccgctc
     5641 ccttgaatgc cgctgattcg cagatcgtcg actatctccg ttctctcggt ttgctggtga
     5701 acgtttacga tgacgagcgg accaaccgcc cagccgccgc ggaaatcgcg gctgagcacg
     5761 acctggtaat ggcgtcgtca acggttctgt ccgcaaacgt ggccggtgac ttccgacacc
     5821 agtcgacgcc attcatcttc tgggagtcct cgctcgcccg cagcgaccgt gaggccatgg
     5881 gcaacggggc ctatgccgca agcgatcaga cccagatcaa cgtgcttgac aacacgcatc
     5941 cggtgaccga aggactgcca acaggcctgg tcgcgcttgt cgacgcgccc gagatgttca
     6001 gttactgcac gcaggagacg gcctcggggg ttcaggtcct ggcggaatcg gcgtcggatc
     6061 cgagccacaa gacgattatc gtggccgaac caggcgctgc cctgctcgat ggcgggaccg
     6121 ccgctggcag gaggatcatg cttcacctgt acgacaccac ctggcagcag accaacgcca
     6181 tgggccggcg ggtcttcacc aacgccgtag catattgcct cggcaagcca acggccgggt
     6241 tcgcggcgtc ggccacctcc ggcgtcgccc caatggagat caccttcgcc gatcagtcgg
     6301 ccggcgcggt cacctcgtgg gcgtggagct tcggcgacgg atcgaccagc tgcctgaggc
     6361 acccatcgca cgtgtaccgg gtctcgggcg cttacacggt cagcctcacc gtgacagggc
     6421 ccggtggccc gagcaccctg acgcgtaccg attacgtctt gatcagcgat cccccggcag
     6481 cggacttgga cggtgatcgg gatgtggatc agaccgactt cggtcacctg caggcatgct
     6541 tctccggtcc gggggcgaac ttcacggagg ggtgcggcga cgccgatttg gacggggata
     6601 ccgacgtgga tgcggaggac ttccgacgat ttcgtctgtg cttgagcggt cccggcgtcg
     6661 tagccgatcc ggcatgcgaa cagcaataag acagcacaac gcaggccgtt ggagggtatt
     6721 gtgaacaggc tcccaggata tctggccctg atactgctgt tggcatcagc aagcggtgcc
     6781 tcggccgtct gtacggtcac cggcgatttc acgatcaact gcgacggcgt gacttatcga
     6841 gcggtcgacg acgagtgcgt gcattatgtg gacctggccg ctcacaacgc cgcccatccg
     6901 ggcatttccc tgcccgacct gaagaaacgc atcatcgaca actacgacgc ggcggcagtg
     6961 ccggcccccg tggtcgtggc catgaccgat tacgtcgatt gtacgcagac cgatcacaac
     7021 ttcaccgacg cgaacatctg gccccagtcg ccttatccca acaagccctc gcgtctgatg
     7081 aacatcagcg gcaaggtatt tcgcgtgacc gccgcgcccg acgccgggtt cgcgacttac
     7141 tactattcct acgacgtcgc cacgggcggg caagcaggtg ttccgcacct gctcgtggcc
     7201 gagttgtcca acgaccagga gcgatacacc agcctgcaaa tccatcatcc cgacgcgatg
     7261 gccatctcgc ccggcctgcc ctggtgtccg ccgtacgccg gcgagccgac ctacaacccc
     7321 tggggcgacc cttggtggga gaagaattac ccgaagatcg agcagggacc cgcattcggg
     7381 cccgacgtcg gtctggccgt ttataccggc cgcgagctgc cggtcgacaa ccagccgttt
     7441 aacatcagca tgatcttcca cgccaagacc gccaccaccc gcgtggccat caccagcacc
     7501 ggctgcaatc tcgttcgcac aagcaccgac ggcggggcgg tctcgcggat gtgggtcttc
     7561 aagttcgtcg acgcgatgga atcgcgaccg ccgacgttga atctgccggc cgacccggcc
     7621 gagcaacggc gcatcggcat ctacatgacc cacccctggt acctctacgc ccattacggc
     7681 acgccggctc ggctcttggg gcatcgccag gaggacctga cgcggatggt aaggcacctc
     7741 aagcactgcg gcatgaatta cctggtcttc aacgcgatca acggtgccga ccgcagtgtc
     7801 aagagttggt acagtggcag cgcttgcttc aactggaact cggcgggcga cctgctggcc
     7861 gaactgccgc cgatcgctga ggccgagggg ctcgaactgg tcccgctcat cacttctctc
     7921 caagatacca acgagagcct gctctcgttg ccaaacgaag cataccagtt gggttccgac
     7981 gggaaccaag tgcgggcatt cggactgccg acgctcgatc cgctgcaccc cgaggtgcag
     8041 accgtgatgt tcagtctgct gcacgagatc gcaagtcgat gtgcgtcgag cccggcggtc
     8101 cgcggcatcg gcatccgggt caacggcaag atcgggacgt gttacacctc ccagcaggac
     8161 tcgccacggg gagctcgcca cagcggttac tcggcgtggg acctgcagca attcaagaat
     8221 gatactggaa gcggcgtgcc gacgtcgccg ccggatgcgg cgtacaactg gctggttgcg
     8281 cgacccgctg agtgggaaag ttggatcaac tggcggtgca cgaggacccg ccagttctgg
     8341 ctcgcctgcc gcgacttgat caagacctac cgctccgacc tgatcttcta cgtgcagtgc
     8401 gatctgccct cggagatgcc cggcaccaat atcgagtggc cggctgagac gccctacaac
     8461 ctcctgcggc atcacggtta tgatccggag atgtttgccg aggataccgg catcatcatc
     8521 tctcgcggcg tgatggtggc cctggaccgc ttcaaggtat ccacccgctg ggccgccccg
     8581 cacggaacga actaccagaa ctaccgcctg ttccactacg ccccgggcct ggccgagttc
     8641 taccgcaccg cccagggaaa agcgtgcgag ttctaccagg attactggga agaaaacgcc
     8701 aacccctact ttgagttcgg ctcgcccgga agcccggtcg gtttcttccg gacgaatacg
     8761 ccggcggcac cagggcgacc gttcttcgag ggcgctgtca tggccgtccg ccgacaagac
     8821 ccggacacca tcacctggct gggctggaac cgaccgacgc tgggccacga ggcggacatt
     8881 cgcaagttcg cgcaggcgtt tcgtgctttg ccggcggtga aacccctgcc attcaacggc
     8941 acgatcgagc cttcacttcc tgaccagatt gcggctcgct ggtatcgcaa tcgcctggcg
     9001 atcatcaatg actcgccgaa tgcacgcacc atcacgctga actttgccgg acccattccc
     9061 ggcggccaga cgctgaccga cgtggttacc ggccgcatgc tgatgtcggc gaccaatccg
     9121 gtgaggacga ctgcttcgtt ccaggcggag gcatatagcc tcaacgtgtt tctctacagc
     9181 ctcggcgaag acgccgattg ggacggcgtt gacggctcgc atgacaactg cccgctcaat
     9241 tacaaccccg accaggcgga cgaagatgaa gatgggtttg gcgatgtctg cgacgactgc
     9301 cctcatacgg tgcccggcgt ggtgatcagc tcgaatgggt gtccggtgcc catccgcggc
     9361 gacatggacc gtgacggcga cgtcgatcag atcgacttcg ggctgtttca gtcgtgcatg
     9421 acctcggtcg gcgtgccgtc gaacgatccg gcgtgcagcg gcgccgaact cgacggagat
     9481 ccgggaatcg gcccgcctga tctgatcatc ctccggaact gcatctccgg cccgatgatc
     9541 cccgcaagca atgattgcat ccgatgagag gcaggagcgc agaaggatgc tgacgcgcac
     9601 agctactcgc tggtcaacgg aggcgactcg cgagcagcta ccgcgctgct tgctcaagag
     9661 gcttcgcgcc ggccccgcca gagccgggca agcccctcag tgcatcgcac agacagcaca
     9721 tacagacagg gcaccacgac aagggtaagc agggtggcaa agccaagccc gtaaatgacc
     9781 gccacggcca tgcgggccca ccactcgctg gattcgctgc gggtgctcca cgagaaggtg
     9841 tgaaaatcgt agctgacgcc ggcggccatc ggaatcaggc cgagtatcgt ggtaatcgca
     9901 gtaagaagga ccggtcgaag tcgggtcgcg ccggcctcca ccgacgcggt gatcaggtcc
     9961 atgccccgat ctcgcaactg gcgggtgtag tccagaagta caatcgcgtt gttgaccacg
    10021 atgccggcca gactgatcac cccgatgccg gtcatgatga tcccgaacgg catggcgtga
    10081 acgagcaaac cggcgaacac gccgaccaat gaaagaatga cggtcgtcat gatgatgagt
    10141 ggaacggtca gcgtgttgaa ctgggccacg agaatgaggg cgatcagcaa cagcgcgatt
    10201 acgaatgcct tggtcagaaa cgcctgggcc ttgtcctgct cctccttctc gccggcgtag
    10261 ctgatctcgt agccgaaagg caagtccttg gcgagagctg caaggcggct ctggacctcg
    10321 gcgagaacct cggtacccag ccggccttcg gcgtcgccgg tcagagtgat cacccgtttc
    10381 tggttgaccc ggctgatcgt gccgaatccg ccggtgtaac tgaattcacc caacgagctg
    10441 agcggcacgg aggctccctg gttgttgggc aaccggaggc gaaacaggtc gtcgacgttt
    10501 tctctttgat cgcggggcaa ccgaacggtg atatcgtatt cgtcgttgaa ctgccgatac
    10561 ttgccgacct ctgtgccgaa gatcgcggtc ttaagatagt tcccgacgat cgccgtgttc
    10621 acgccgagca tcatggcccg gcgccggttg acgcgaaacg acagctccgg ccgcgcagct
    10681 tcatgatcac ttcgaagatt aaccaacccg ggaatgtcag cgatgagctg tctggcccgc
    10741 tcgctgagat cttcgagggt cttgaaatcc tcgcctatga cgcggacggt gacggccgcg
    10801 cctgtgggag ggccctcctc ctctttggaa accttgattt cggtacctgc gatgtccgac
    10861 aaggcgtcac ggatttcggc aatggcttgg gccgatgggc gctcgcgaac tgcataatcg
    10921 cggaactcca gcgtaatggt cgccaggtgc tcgcccatgc tgcccatgcc tcctgacagg
    10981 aaatcgccgc cgcccgaacc gacattggtg atcacgcgtt gaaactcggc cgcaaagggc
    11041 tgaaggcggt cctcgaccaa ccgggtcagg ctgtcggttt gcctgatgtt cgttccctgc
    11101 gggcaacgca ggctgatgat cgcgcggcgc gggtcagcat ccgggaagag ctcaatgccg
    11161 tgggcccgtt tgccatagag catcgccagg gtgatcagca ccaggaaagc cagcgacggc
    11221 gtggtcagat aatgagtgac ggccacgttc agcaaccgtc ggtaaccgcg catgaagcgg
    11281 ctttgctggt ttcccttctt gagccggccc ttgacgaaaa tggagctgag cgtcgggttg
    11341 atcaccagag ccacaaacag cgaagacaac aaggtgatga tcagggtgat gggcaggtat
    11401 ttcatgaact cgcccatgat gccgggccag aacgccatgg gggcaaaggc ggcgatggtt
    11461 gtgaacgtgg aagtgatcac cggccaggca acctcggcgg tggcgatcag agccgcctgc
    11521 acgcggttgc ccgtcagagt gtagtggcgg tagatgttct ccacgatcac aatcgcattg
    11581 tcaacgacca tcccgacggc cataaccagg ctgtacagca cgaccatatt aagcgtgtac
    11641 ccgagcagtt gcaggaccac catgctcagg agcatgctca gggggatttc gatggccacg
    11701 atcaggctcg gccgccagcc catggccagc atcagcacga ccaccacgag tatcatgccg
    11761 gaggccagat tgttctccag gtcgacgatc gtcaggcgta tgtctttgga ctggtcgagg
    11821 gtgatgtcga aacgcacgtt ggccggcacg cgtcgctgaa actcggcgat gacatgctta
    11881 acggcctcgg caatgaacaa gatgttctcg ccgacccgtt tctgcaccgt gaccgtgatg
    11941 gtttccgacc cgaccagatg cattcgtccc gcctcgtcgg ccacgggcgt ccacaggcgc
    12001 gcgtaactca cgcggtcctt gaacgtgtcg cgcacgccgg ccacgtcggt caggtaaatc
    12061 ggcttaccgt tgcgcacggc gatgaccagc ttgtccacct cctccggctc gacgaactcg
    12121 gcggggacgc gaacgttgaa cttggtgccg ggcgtctcca ggctgccggc ggagatgttg
    12181 acgttctccg ccggcaccag actgaggatc tcggcgatcg tcaggccgta ggcactgagg
    12241 cgattctggt cgatttccag gcggatttcc cgttcaagac cgccgagcac gtcgcagtta
    12301 agtacgccgg gaacggcttc caaggcctcc tccaggtcgt ccgcgatcag cttgagggcc
    12361 acgggcgaaa tgtcacccga gatgctcacc atgaggatgg gaaactccgc aacgttgatc
    12421 tcggtgaggg tcggctcctc ggcgtctcgc ggcaactcgg gcttggcgcg gtcgagccgg
    12481 tcgcggacgt gttgcatcgc gtcgtcgatt ttgacattgg gcatgaactc aatggtgatc
    12541 agcgacctgc cctccgcgct ggaggaacga atctccttaa cgcccttgag gcccgccaac
    12601 tctttctcga ttttcagcgt caccgtggac tcgatgtcct gaggggacac gccctcgtaa
    12661 accgtcgtta ccaggatgaa cggaatggga acgtcggggg ccgcctcgcg cggcagactg
    12721 atgtaggcat aaatgccgaa cacggcgatg agcacggtca gcacgccgac ggtggtacgg
    12781 ttgcggatcg caacatctga caggatcaca gcatattctc cacgtcgggt acggacggcc
    12841 tgttcgctgg tgccgggagg ctgggccgag tggcggttgc ttgcgcggtg tgacgctgcg
    12901 gatcggaagg acgggggttc tccgcgaccg gtgacggatg actatcgttc tcagagcccc
    12961 ccgcatccgg cgccaccacg acgttcaccg gttggccggg gctgaccagc cgatgacctt
    13021 cgacgatcaa caggtcgccg ggttccagtc cgcggctgat ctggacgctg cgaccccgaa
    13081 tgaagcccat ctccacggtg cgccgctcag ccttgccgtc aaccacaacg taaacctcct
    13141 tgccctcctc gagcggaatg acgctcgcga gtggggccat gatcacattg tgcaagatgc
    13201 ggcgagtcag tcgaacgtcc acaatctggc cgctgcgaag gcggtattct ccttgaaagg
    13261 aagcggactc ggcagaatcg gagatccggt tctgcacggt cacttcgatc cgggtggtgc
    13321 gcgtgacctg atcggcaagg gcgttgatgt aagtgatggt tccgaccagc gggttttcat
    13381 cccggggtga acgcagcacc tcggccgttt gaccgacgcg aaggtagcct acgtcgcgct
    13441 caggaacgtc ggcacagacc ttgaccttct cgatgtcaac gatttcggcc accgtgtcgc
    13501 ccggaacggc atactcgccg aggtccatcg gccagtcatt cagaatcccg ctgatcgggg
    13561 cggtgatcgt ggttcgagcc agcgagcggg cggcctcgtc aaggtcggcc ttgctcacgt
    13621 cacgccgggt gcgcgccagt ctcatctccg ccgacgatgc gttgccccgc tggtacagag
    13681 aaagcatgcg ttcgtactct tgttcatcgt actcgtactg ggcgctggcc cggtcgaacc
    13741 gggcctggag tagatcacgg ttgagaatga caagtgggtc gccgaccgtc accggctggc
    13801 cttcgccgat ctggctgccc accggaagga cgcttccctg ccaacgaacc tcggtagagc
    13861 gcgttccgta tctttcgatg cgcccgggca cctccgccgc gacgcgaacg gtcgcataag
    13921 gctgcacaac agcggtgatt tcgaacgagt ccgcgatctc cgcgacgggc tcaacgcgcc
    13981 aggtcttcac attgaccgga acgcgctgcg ggggaggcgc ctgcgtctgg ggggcgggca
    14041 tcgacgcaac gatgcccacc gccgcgacgc cggccagcag aaggcaaccg gtgatcagcc
    14101 tcgcccgtcg tcgacctccg ctcgcggtct gttgcctcgg tccgatcgcg gtgctcatgt
    14161 cttgttcctt tcatcccgaa tcacttccgt ccgggcggnn nnnnnnnncc gcagagtcgt
    14221 ttccttccag tatcgattcg tcccggagga tcccccgtaa aaccgcccgc aacgtcgcct
    14281 ctcccaacgc gtccgcccct ttccaattga accacggctc cttttcggcc cgtgtgacct
    14341 gcaacgatgc aacgccgtgc ccgcccgccc acaaaacctg ggccaccagc tccgggtcgt
    14401 gctgcagatc atcccggacc cagccttgtg cgatggcctg ctggacgcat tccatcagga
    14461 aggcgtatcc gtcgcgggtc gggtcgcccc gccctcgctg tctcatctct tcctcgtcca
    14521 tctggaccat ttccggcgtt ggtttggtca tgaacatcag ccgaaagtgg ttgggatgct
    14581 cgatggcaaa ccggatgtaa gaccgcgcca acaagcagat gcgttggatg ggattcgcca
    14641 catccgcaaa ctgttgagcc tggcgggtga aggcctcgta gtccgtatgg cagagttcct
    14701 tcatcaagtc ggccttgtcc ttgaagtaca catagatggc cgtgggagag tattcgatgg
    14761 catccgcaat tcgacgaacg gacacagcct cgtacccttc gcgggcaaac agctcgcggg
    14821 cggcatcaag tatcttttgc cgcgttgcat ccttagctcg ttctctacgc tctttggttc
    14881 ccatgcactt tcctcggcaa acagcataat cttagttagc gctgttatct ttgtcaacag
    14941 cgcaaactct caggcaggaa ctctatgttc ttttcttata aacacttaaa cccaacggac
    15001 agaaacctga acaagatccc tgtttccccg cacgtgccgt caatgcctga gacgacagca
    15061 ttgccgaagt acgcctgcgg aattgcggtg cctgcgggct tgccctaata tcgcctcgcc
    15121 gaacccatga ggactgaact gatggtgaat cagacttcga cgccctgcgc ggccgaattg
    15181 ctcaacctga tccgggaggt gccggattac ccgaaacccg ggatcctctt ccgcgacatc
    15241 acaccattgc tgcgtgatcc ggccggtctg tcgctatccg tggagctgat gacgcagccc
    15301 tatcgcaaca ggcacattga catcgtcgtc ggcgctgagt cgcggggctt catcttcggt
    15361 acggctgtgg cccggaacct ctcggccggc ttcgtaccca tccgcaaacc ggggaaactc
    15421 ccctgcgcca agcgaggcgt gacctacgca ctggaatacg ggaccgacac gctggaaatc
    15481 catgaggacg ccatcgagaa agggcagcgg gtgctcatgg ttgatgattt gctggcaacg
    15541 gggggtacca tgaaggcctg ctgcgaactg gtggagcagt tgggcggacg aattatctcg
    15601 gtcgtgttcc tgatcgagct ggcgggactg aacggccggg agaagctccg gaagtgggac
    15661 gtttacagcg tgttacgtta cgactgaaca agccacggaa tcagccgcgt ggaaacctgg
    15721 tactggacca tattcgccct gttgttcggc gcctgcacag ggagttttct caacgtggtc
    15781 atctaccgct ggcctcgcgg gatttcgctt ctttcgccgt cgcgctcttt ctgtccgaca
    15841 tgcagtcagc ccatcgcctg gtatgacaac cttcccgtcc tgagctacct gcgactgggc
    15901 ggtcgatgcc gaagttgccg caatcccatt tcgctgcgat acccgtttgt tgaactggcg
    15961 acgatgctcg tcttcgtgat gactttcgac gccttttttg ccgcccgaca acgtttcggc
    16021 gtaggtgaca tgggcgacga ctgggtgatc ctgatcgctc actgggcgtt gtgggccggc
    16081 atgatcgcct tggcggtcat ggatctcgaa gcctacatgg tcgacgtcaa cgtcacttgg
    16141 tcgctcctgc tgatcggcct gatcggtcat gtcttctgga cgcctccgag gagcgttgaa
    16201 tggatccgtc cgtctccgac gctggccgct tttgccttgg cggtcactgt cggtctggcc
    16261 gttggtgcct tgctgtttct tcgggggccg gaggagccgt ccccggaaca atctgccgac
    16321 gaggaggcgc cggccgcgcc gtcggtcaca tcgccgagag gtcgcattcc gatcggcttg
    16381 ctggtcgctg ttgtcttcgt ggtcggctat gccgtggcgg tttcgctgat gggcgagcaa
    16441 ccccggctcc gtcgcccgca attgcctgac cactcggccg tcttcagcca gtccgcggag
    16501 gcttcacaga tcgatgccgg cgcaattcgt ctggcgacgg gcttagcatt agccttcgtc
    16561 ggtctggtac tggccgccag ccaaccaagt cccgaggccg accaggaaat cgtggatgcc
    16621 atccacgaag aggcgccaaa gtcccgtcgc aacgccttgc ttgaattgag actactgctg
    16681 cccgcctttg tccttgtggg cgttttgatg tttgttctcg cttcctcaca gcggacatca
    16741 ttcgagacgc acgtccgcga cgttgtccac tggtcgccag cggggcactg gaaacctatt
    16801 tggggactct ccaccgcgct gagcggatgg gtgatcgggg gagcaatcgg gtggtttacg
    16861 cggatcgttg gcacgctcct gctgggcaaa gaagcgttgg gaatgggaga cgttcatatc
    16921 atggcggccg ccggtgcggt cgcgggttgg ccagtggtcc ttataggctt ctttctcgct
    16981 gccgtgcttg cgttgctggg cctgattgtc atccacttcc gtcgtcaaag ccgcgctcta
    17041 ccgtacgggc cctggctggc actggggttc ttggtcgcga gcgtgtacca ggatcgaatc
    17101 ctgcttttcc tgcaggtccg ctggctgctc gaacaatcgc tctgaattgc cgaaacctcg
    17161 tcaccaacag acggattaac ggcacgaagg ctaacgcact ggttgcaggt gcatctcgaa
    17221 tagcgttccg gctcgcctgt gaagcccaaa acggttagac ttgtcagcac tgaaggaata
    17281 ccttttttct tcaggttgca gaggggattg acgatggcga tacatcgaag gaccattgtg
    17341 gaatcggatg aaactgttgt cttgtgagcg gtacgggacg caaagaaagg agacatgcca
    17401 tggcgcgtct ggtttccagt ctggtgaggc tgttttccct cgtattggtc atttcgttgt
    17461 tcggctgctc cgatcagctc aagcaatgcc agcaggatct gacgaccgca aatgaccggg
    17521 tgcaggaatt gcagaagctc tacgatcagg ccgagcgcgc tcgcatggcg gccgaggacg
    17581 aagttactgc tctgaaagcc gaacgagacc gcctcaaggg tgagctggcc aagaaacccg
    17641 agggttggga caaggtgccc ggaggggcat tgatcaacat cgaaggcgac attctgttcg
    17701 attccggaaa ggccgagctg cgcaaagaag ggatgccgac tctggacaag atcaccgatg
    17761 cgatcaagca gcggtacccc aaccacgaga tctacatctt cggtcatacg gacaacgacc
    17821 cgatcgaaaa gagcaattgg aaagacaact acgaactaag ctgccagcgg tcgctgaccg
    17881 ttttgcgtta tctcaagaac gccggcgttc gaaactacct ggcggcctgt ggctggggcg
    17941 agacgcgtcc ggcgcagacc aacgacaccg aggaaggcaa acaagccaat cgccgcgtgc
    18001 agatcttcgc tctggatccc aagcttcgcg gccaatgaaa gtgcggcccg catcagcggc
    18061 aacatgacgg gctcgagaga gctgctaaag acaacagacc gacctgcacg ggagcctatc
    18121 tttcgtgcgg gtcggttgtt tctttgggca aacggtggtc gtgccggaca atctcagtgg
    18181 atgagagcag gaccggttgc cgacctttca gaacatcccc agcggaatgc gatcgggtgt
    18241 gatcttgccg acgacaaagg gcctctcgac ggccgcggcc tggacctcgg ccgctacctg
    18301 ttgagaaccc atgaccaagg cttcttccag gctgcgacag gcaggcacgg tgaccgaggg
    18361 gtcctcgcac tcgaagtgcc actgcccccg atggtaggcg agttgaacag gcaccggctt
    18421 gctgatcgcc aaagcgttgg ctttctgctc gacctcgatg gttagattga ccctgaaatc
    18481 catttctgac ggctccctgc ggcgaggttc gttcatcttc ctcgggttct tccatcggct
    18541 gatttcgtgc ggcaggtgcg ataaaagcgg caatgcgaac gcaggaattc cagagcaccc
    18601 catccaaacg ttgccctgcc tattgtgccc ctgagtaagt accgtcgcct caggccctat
    18661 aatcccaaga ccggattcat gcctgggtgc aagccctcgg gagaggcatg cgaaaacacc
    18721 attctttggc tcagcctgct aacccgacgg cattttcgga ccgatccaac ggataacaag
    18781 acattgcccc gacgaagtgt cgggcgtccg aattgacggg tctgtacgcg ggttgggagt
    18841 accgatggac accacagttc tggaactgat caaacgctct gcggcgatcc cgtcgatgcc
    18901 gcaagtggcg gtgcgattcc tcgagatcat acaagacccg gatttcgatt accgcgaagt
    18961 ggtcgaggtc ttgtcttccg accccgggac gacctgcgag atactgagat tggcgaactc
    19021 cgccttgttc ggcgtcacgc gcaaagtcac cgcattgccg catgcaatga ccctgctggg
    19081 catcaagcga atccgctcgt tggtcctcgg gcgatacatt gtcgagagcc tgaacagaaa
    19141 cggatgcgca agcatcgaac cggcctatta ttggcgacga tcgctgacgg ccgcggttct
    19201 cgcggcccgg ctcgcggacg cgatcctgcc cgaccgacgc gaagaagcgt ttatctcagg
    19261 attgctggcc gatgtgggga tcgtcattct ggatgccgcg tttccggaca agtaccgtac
    19321 aatcgccgag caataccgcc ctggaggcaa ccccgatctg gctctgactg aagaaatcct
    19381 gcttggcacc acccacggta aggtttccgc gacggtgctg gatcactgga agttgccgga
    19441 agccatctgc gaggccgttc attggcactc atgggaatgc cgcgagcaag gtcgaaatgc
    19501 cctggccgat ctgacgggtg cggccaaccg gatcagcaca tgggtatgcg aagccccgac
    19561 ggacctcgac gcggtcgtcg cagactgccg cgaaatcgcg catgtcctga atctggcacc
    19621 ggagacgctg gctcgctgcc tcatggagat tgaggaacac gtttcggatc tctcgtcgct
    19681 gctcaggatc gaggtcgtct cctcacaaat gtatgaagtc atcaccacac gaatcagtga
    19741 ggggctgacc catctcgaaa cggtggaggt ataggcgagg gttcaggcag tccggcgacc
    19801 cgcggagtcc tgcgcggagg cgcccgcgcg gggctgtctc tcgacggccc ctcgtgactc
    19861 ccctcgcggc atcctcaagg ctctgccgct gatctacact tctacgatct catagcgttt
    19921 ggatatgtag gtgcaaccga cgtcgacgca atcgatatgt ccgattcgca tgcggtgctc
    19981 gcgatggctg tggccgcaga acacgtatcg caccttcggg catcgcagaa ggagctcgcc
    20041 gaagcgcgta cttcccagga aggctgaccc gaacgcccag ccagggtggt cttgatgcgg
    20101 aaccagctcc tcaaagggca ggtgatggat accgaaaatg atcgtgcggc agatccgctc
    20161 cagttgccgc aggtgggcat cggcgcgttc gaccaaccgc cggcagaaat cgacgtcgct
    20221 catcgggagg cgcacgtgct cgccgtccat ccagcgcgtt ccgatgcgca aagcctcttc
    20281 agggacatcc gagaggtctg ccaccagatg ggcgtattca tccaggcgag aggccgcgcc
    20341 aggggcgatt ttggcttcgt aaaatcgcag cgggatgtgc aattgctccg agcggaaact
    20401 gtagtcatac cagccgatcg aaccggccag gccgacgccg tccacctcgg ccggggccag
    20461 gccgagatcg tgaaaccctg catcttgaca gatttggggc aacacccgct ctaagcggtc
    20521 aagggagtcc tcgccgggat tggtccagat gtcgtggttg cctgcaacga agaatctgcg
    20581 acccttgaat ctgtcgaaaa gatgaaggcc atcgcgtaca atatcggggt cccggccgcc
    20641 gacgtcgccg agaaccagca gcgcgtcggc atcgagtccg cagatctgct cggccactct
    20701 gcgggccggc gccatgctgc gggcaatgtc gtaatgaagg tcggcagtaa ccacaagcct
    20761 catgcggtac ctccgggcgg tgagtgtacc ccaggcagca gaaggccaca acgtgaagga
    20821 attgcaggga gctttggcct ggcacgtagg ggctctggaa gtccctggcc atccctttgg
    20881 ggcatgagcg ttgaatcgaa accgctcctg cggggtctga gagaatcatg accgacgtcc
    20941 tggagattga gaaccttcac aagagcttcc gactgggcag ccaggtaatc cgggcattga
    21001 acggcgtttc gctctcggtg gcccagggca cgctggcagc catcatgggg gccagcggtt
    21061 cgggcaagag cacgcttctt cacatggcgg gcgggcttga cctgcccgac tcgggaaccg
    21121 tgagaatcga gtccaaggat ttgacgcgca tgtccgaccg tcagcggacg ctgtttcggc
    21181 gaaagcgcat aggcatcatc ttccaggctt acaacctgct gcctactctc acggccgagg
    21241 agaacgtggc cgtgcccctt ctgatcgacg gcctgaacag cagccagatc agatctcgcg
    21301 tggcggaaat gattcgactg gtgcacctgg agcatcgcgc cggccacaga ccacaggcga
    21361 tgtcgggagg cgaacagcag cgcgtggcga tcgcacgggc tctactcaat gacccggccc
    21421 tgatcctggc cgacgagccg acgggcaaca tcgactcgat cagcgcggtc gaggtgtggg
    21481 aactgctccg tcggctggcc catgagatgg gcaagacggt tctgatggtc acgcacgagg
    21541 ccgcggcggc ctcctacgca gacaggattt acgtgctcaa agacggacag atcatcggta
    21601 ggatcgaggg aataaaacca agtgatgcgg cactggtcgc aagtcggtat acggaactgg
    21661 cgcgttaagc cggggcgaac cgccggagcc gtcggggcca ttgccctcgg cgtgggcgtg
    21721 gtcatctggg taaccagtgc ctatgaatcg gtgcgcctgt cgctggaaga ccaagtctgg
    21781 ctctggatcg ggcgaagcca tttgtcggtg gaatccgtct tcgggcaaaa cggtcaggtc
    21841 aatcaacatc tggcggacaa gatcgccgcc ttgccgaatg tcgctcacgt tacctgctgc
    21901 ctgcaatact acatgtacct gcaaccgttg atcgaagacc ctgaaaccag gaagcttgtg
    21961 gagggcagcg gtgtctcggt gatggccatg ggcatcgagc cggcaacgga gtaccacttc
    22021 cggcaatacc gcagggagcg tcctggaaac ggtggtcgcc ttctgacagt ggacgacgcg
    22081 gacggcctgc tggtggaacg tcaactcgcc gagcagacag gtctggcgat cgggagcaag
    22141 gtcctgattc gctgccgcgg tccggaatgg atcaaagcga ccgagcagtc cgcggttttt
    22201 acgattgtcg gcgtcatcga acaccggcgg gtggccaagc agcagcctcc gctgatcgtc
    22261 gggcgtcttg accgcatgca ggaactgtcg cagatcagca aggatgttgc ccgcgtcacc
    22321 cgcattgaca tcctgctcca tgactactct cagaaggcgc tgcgcgccac acagcaacag
    22381 gtccgccaga tcgtggaccg cgacaaacag ggcttcctgg tgaattccag cgaggcaaag
    22441 ctcgcacagg tggaggatgc ggagcagcaa acggcgtttg tcatgggcct gctgtcgagc
    22501 gtagcactgc tgaccggctt tttcatcatc ctgtcgacgc tgagcatggg aatggtggaa
    22561 cgaatcgctc agctcggtat gctccggtgc ctgggcatga cgcggacgca gacggccatg
    22621 attgtactgg ccgaggccct gcccgtagcg ttgaccggcg tcctggcggg tgtcccgatc
    22681 ggtgcaggat tggccgggct cagcgtgtgg ctggcgccgg aatacattgg tcaatgggtc
    22741 atcagccggc caggcatggt tctggccctg gccggcggtc tcatcacgac gcttgccgga
    22801 gcagccattc ccgcggtcca ggcgatgcgc gtctcaccgc tggccgcctc tcgaccgcag
    22861 tctcagccgg ctcccgggct ggtgacctgg acggctggag ctgtggggct ggcaatgatg
    22921 gccgggtact tctacgccat tctgtggacg ccgttgatcc agcatctccg acacccggct
    22981 tatccacttg tgctgatctc gctcctctac ggcgggtgtg cactgatcat gcccgccgcc
    23041 attcgacttg tcgggcaggc ggccgtaatg gccgccgccg ccgtgctgcg tgttcaatgg
    23101 cgtctgctga gcgaccaggt cggccgggcg gcgtggcgat ccgcggccat ctgctgcggg
    23161 ctcatggtgg gcctctcgct gatcgtctcg ctcgtggtat tcggcaagtc catagccggc
    23221 ggctgggatt tcccgagcca gttcgccgag gcgttcgtgt tcatcagtcc gccggtggac
    23281 aggcagaaag cccgacggca ggtacaagac ttcaaaggcg tgaagtcaag ctgcctggtg
    23341 aatgagggtt tgcgttgcac agtcttcggc cggagtctgt tctacttccc ctggtgcacg
    23401 ttcgtcgccg gtgatccgga tgaattcttc agaatcgcga agctggagtt cgtgcaaggc
    23461 aaccagcaag acgccgaggc caagctgcgt aagggcggtt acatcctggt gacgcccgaa
    23521 ttcacgcgca cctatatggt cgacgtcggc gacacggtca atatccgcat gaatgaggcc
    23581 aaacgaagaa tgcaccgctt cgagatcggg ggagtggtga catcgcccgc gctgcagatc
    23641 gccgccaact acttcaacgc cagcggaatg ctcacacaac attccgcgtt cgtggtcatg
    23701 ggcaccttcg aggacgcccg acggttgttc gaagttcccg atgtagccag catgtttctg
    23761 atcaacttcg atctgcccca gacatctccg ccggccgagt ttgctgaaga aatgcctccg
    23821 cgagtaagcg atgccggcgc gctggccgac atgctcgaaa cctggcgtgc ctcgttgccg
    23881 gagcgatctg aggaactgga ccggatcgtc gagcaatgca ggcctctgcg aaccgccggc
    23941 atgccgattc attggctcca ggtaccgctg cttcgggtat ttcaggaatc gctgctcagc
    24001 ggcccgaacc ccgcctgggc ggacctcaca cccgaacaac ggtggcgcac gttccgggaa
    24061 gaactggtca tgcagcttct ggcggccaag gtcggctcgt cgagccccct gcacgcctca
    24121 gcccgggcac tgaaggatca gattgatcgt gatattcaac gggccatgct cctttttgcc
    24181 agcgtcccca cggtggccct gttcatcgcc gccttaggag tggccaatct gatgatggcc
    24241 aatgtcacca gtcgaacgag gcagatcgcg atgttacggg cggtgggtgc gaccaagtgg
    24301 caggtcggac gcctggtgat cggcgaagcc ttggtgctgg gactgctggg cagcctggga
    24361 ggagtcgcgg tcgggctcct tgccgcccac ggagtgaaca agattgtgta caaagtgtgg
    24421 ggctttgctc tggagaccac ggtgccatgg cattgggtcg ggttaggcgt ggccttcacc
    24481 gcggccatct gcctgattgc cggccttctg cccgcccgat atgcctcgcg aaacaacatc
    24541 atcgacgcga tgcaaacgac ctgaagtggt gatcggaagt cgtgctttgg gagcaagaac
    24601 ttgaatatga ccgtgcacgc tgtctcgttt gcgtcggcta ctcttttctt tgccggtctt
    24661 gccgcaggca cggagaagac gatcgtccgc ttccggctga tggacaccgc tgccggacag
    24721 accaccccag ccatggtctg tgtcgccggt gccagtgacg gcgaggcccg aaatcgcagg
    24781 cgataacacg ccacgcggcg agggggttct gacaggctgg ccaaggccag ggcattctac
    24841 gatgatctgc gacggaagct gaataaggga aagtgaaccg ccaaagcgcc aagaagaaga
    24901 ggtggcatga gctcgccgca gcgggctcgt tgaatcggct ggggtgaaac gcttaaacag
    24961 tcatgaccgg caaacgaaaa cggcctatcg ccctcctcat ggtccttggc gtcatcgcgg
    25021 cgacgtttta tttctgtttc attgaccgag cctggcgaca tacggaaaga cacggatggg
    25081 ttctcgactt ctcgtacgtg cgcctggcat gcccgacctg tctgggccgc atcgagaggc
    25141 tcgagtacaa agggcaacct atcaaggcac cggcggactc gcatctccgc atctcgctgc
    25201 tgacacccgt tgtctcggtg gagtcttggg acgagcaggg ccgatggcgg tatcatggcg
    25261 acgagcgcgt cgccatggtt actggagaac cgttgtcgga ggagacgatc tcggaaggct
    25321 tttacactgt cgatctctca atacgaggag gttttccgcg ccgtcgaccg gggacgccgg
    25381 aatcatggtg catgttgtgc cgttgggata tcgccaaagg ctactggctg gccccggaaa
    25441 agtatcagca gctcttgcag atgctgctga atgacgggcc gcaggaaacc aggcaagagc
    25501 aggctgagag atcgacaaca gggtcggcac cggcggagaa ttgaggcccg gacggccaaa
    25561 agttgtggcg acgcccggat ctcggcgccg ccgccgtcgg gcccggatcc cgcaacgatc
    25621 agtggcaaag agattcgccg ccgaagcggt gcgagaaagg tttcttggac cagcaggctt
    25681 tgcggactcg tccccgggga attcgctttc ctgctttccc ggcgtgttgg cggtttgctt
    25741 ttcgccacct ttgtttgcag taggtgaacg atgacaagaa tccgtatctg gacatgtgcg
    25801 gcaatcctgc tcggctcaac cccggcattc tcggagatcg aacgctggta catgaacgag
    25861 gagtggtacg actggcacgc ggcttcggag tttgcggtcg acaagatcgg gctcgtgagc
    25921 gtcgaggtcc attttgtcgg gccgggggcg gtgacgccgc gagggctgta catccgcggc
    25981 gatgccgacc agccgctcga gcgaatcgag gtcctgggtt tcaaggacca tcgcaaggag
    26041 ccctggaagt acgagccggc cgtcatcaag gccgaaggga cacccacagg caagaagtgg
    26101 gttcgtttgg ccaccggggc caacccgcga ggctttgagc tattgacgct caagctcccg
    26161 ctgggctccg actcatcggt tcccaagttc gattacgtgt gtgtaagccc caacgagcgt
    26221 tacatcgccc acggccgggc ggagcgatgg atccgcgacc gtgaaaaggg caaggaggtc
    26281 cgctgcggca tgcccctggg cggcatcggg gccggcaaga tcgaaatcgc ccgcgacggc
    26341 tcgtttcgca atatcaccac caacaacaac atcgacacgc cgttctacca tcccgacctg
    26401 tgcttcatgg ccgcgagcgt gaacgggcac gcgagaatcc tgcgcgacga acctgctgtc
    26461 ggcttgacgc cggttgagaa gatcgatttc aacgcccgtt atccgatcgc ccaaatgact
    26521 ttcaccgaca aggcatggcc gattcccatc gcgctcactg cctggtcgcc gatcatcccc
    26581 ggcaacatca acgactcgtc gttgcctgcg gctgtacttg agtttaagtt ggagaacaca
    26641 accaagcaca gacagtatgt ggccgtcgcg gtctcatggg agaacctgat cggctccggc
    26701 ggccgacctc agccagggag tctgtggggc aagaccggcc actatcttcg gttccgcgag
    26761 gatgagggca atacgacgac ctcattcagc ggaggcatgg atggtccgcc ggccggcttc
    26821 attttcagcg gcggaccgaa aacggagaaa gactcgcaag ggaatcatgc gctgatggta
    26881 tggcttcctg acttcatggg cgagttcggt ccgggctgga cactcatgcc ctacaatgcg
    26941 gagactggtc cgcgagagtg gaaggatttt gggcggcttc ccccacatga cctgcgaaac
    27001 gtatcgccac aacctcatac ggccggcgga gtctccatca ggggcgagtt gttccccggc
    27061 gaaacagtag ttgttccctt catactcacc tggtacatgg accacttcca tcagctcggc
    27121 aaggaggatc tcggccacta ctaccacaac cgcttcaaga acgtacggga ggttgcggag
    27181 tacgtgctca agaatcgtga acgtctgttc ggggagacca aggctctcca cgacctcttc
    27241 gaccgcagca acctccccgc ctggttcaac gacatgctgc tcaacgacct ctatgttctc
    27301 agcaccgaca cctggctgac caaggacggc cgcttcagcg tcaacgaagg cgcgaccagc
    27361 atgttcggtt gcatgggtac catggatcag aagctctacg cttcacacca cctggccctg
    27421 ctcttcccgc agcttcagaa gcaggaactc cgccagttcg ggcaactgca gaacgacaac
    27481 ggtggcatca cccacgacct gggcacagcc gacttcgccg ccaagctcaa ggccttcgac
    27541 tggccggacc tgtgctctgc gttcagcatt ttgtcgtacc aggtatatcg ctataccgga
    27601 gacaaaacct tctgggaaga gatgcggccc aaggtgctca aggccatcga ctgtctggcc
    27661 ggcgactggg acccggacag acttggcgtc cccggacgtg gcagcacctt cgacgatgaa
    27721 gacagctacc gcattttcag ctacaccacc ggcctgtacc tgtgcacttt gaagcttgcg
    27781 atgaccatcg cccgcgagat gggcgatgac aagctgctgg ccacgtacca agcccgctac
    27841 gaccgggctc gcgacctggc gatgaaggag ctatggacgg gcaactactt ccgctacggc
    27901 tcatcgccgc ctccggagaa caagaggacg gacgccagcc atttctcgca gatggccggc
    27961 gagttctggt gccgtctgct cggcttcgac ggcatcttcg acgagcaggt ccgccagacc
    28021 gccctgtcca acacgctcgc cctgcattgg aacgagaact tcaagctgcc acccaagatc
    28081 gtcacccccg acggccggct cttccctcgc gacggcgagc accgtaacgc tccggtgagc
    28141 tggccgatgc actcgcgggc cctgctgtgc ggcagcgcgt tcctcttcga catggccccg
    28201 accggctggg acctgatcaa ggccatgcgc gacaacatca tcgccgcgaa cggcccggac
    28261 ccgtgggacc agagcctcta ctacgacccg atcaccgccc ggatcgactg gggcgtcttc
    28321 tacatgaccg ccccggccag ttggctggcc taccaggccc tggccgatac gcattacgac
    28381 gccgtcagcg gcactttgac cctcaggccg agagctctgg ccaaagtctg ccccggcaaa
    28441 tggccgatca tcacaccaac gttttgggcg atcggcgagg tgtcagccga cggcaagaag
    28501 ctgtcggttg acgtcacgaa gaccttcgct gatggtatgg cggtcaacaa gattctggcc
    28561 gatggcgaga tcgagcaggt gtgggtgact tcgtcagagc ccggcaagca gattgagaag
    28621 gtgagtatgc gagatctgat gcctctgcga gcgggcctgc gatgccagat cacgctgaag
    28681 tgaggtcttc gggagcctca tccggccggc agatggagaa tcatgagatg aagaagaata
    28741 tctcacgtcg aacggtgctt gctcgtgccg gcgcggctgc cgttggcgcc gcgtgggccc
    28801 gcacgttggt cggccaatcg actcgtccag tgggctcgtc gctcggtgcg ggtgagcgcg
    28861 tcggggtagg tttgatcggc tgcggccagc gcggctggca gcttctcgat ctgttgctcc
    28921 ggcaggagga tgtcgacatt cccgcagtct gcgatgtcga ctcggaacgg gtgggtttgg
    28981 cagctcagcg cgtcgagaag gccggtcgtc gcagacccga cgcagtcgct gattttcgca
    29041 agatgcttga ccgctcggac gtccacgccg tggtcattgc cacgcctgat cattggcacg
    29101 ccgttcagca cgtctatgcc tgcacggccg gtaaggacgt ttatgttgag tcgcctgtcg
    29161 gccacaatgt cctcgagggg gcggcgatgg cccgaataac aaggaagcac ttccgagtcg
    29221 tccaagtcgg catgcagcac cgatcggccg cttggttccg tgacgcggta cagcaagtgc
    29281 gggcaggcac actgggcaag atcgcccaga ctcgcagttg gaactttgcg aagctcaagc
    29341 cggtgccccc ttccgacgac gctcaaccgc cggcgtccct cgactatgac aagtggctcg
    29401 gccccgcccc ggtgcggccg tacaacccgg ggcgcaccca cggcctgttt tacaactggt
    29461 gggaatacgg cggcggcatg ctaactcgct ggaacgttca ctaccaggat ctgatccact
    29521 gggcgatgcg ggttaacggc ccggtgagcg tcaccgccgt cggcggtaac cagggtctcg
    29581 gtgatttccg cgagacgccg gacacgatgg aggtgatctt cgagtatgac acgcaactcc
    29641 tgccgcgtcc gggccgtttc acgcacgtct actcactgag actgggtaac gggcacgccg
    29701 tgtggggtcc gccggtgcct gcacacaacg atatgttcga tgcgatcagc tccatgcaca
    29761 gcggggcgct cttccacggt gaagaggcga ctctcctggt cgatcggcgc gggtcggcgg
    29821 tgctccctgc cgcactgcgt caggacaaac cgattgacac ggcccccgtc gagaacgcca
    29881 atccaccgtt cgatgcgttg accctggcac acctccagga tttcgtgagc tgtgtgcgga
    29941 cccgtgcgga gccgaaagcc tccatcgagg tcggccagta cgccgccgcc gcctgtcacc
    30001 tggctaatat cgcatatcgc acgggccgca ggatctactc caagcccggt gaactggctt
    30061 gtttcgccga ttcgacgtat gagacggttg acacgcaggc cacggagatg ctccgtcgca
    30121 ccgcctatcg cgagccctac gggccaccaa gaacctgatg ctacggcaga accctcagac
    30181 gacccgagcc ggtgacggca aggaggaagg cgaacgtgat cagacgcggc aagcggctca
    30241 atcttgcggc aacctgcggt ttgactctcg tgctttcgac cggcgcggcc catggcgatg
    30301 tcagggtctg ggaagagcag gtggtcgtcc cgacctaccg ggtgggcgcg ccggatccca
    30361 atccgatctt ctattcgaag gagtcctatc agggtgctca gaagcgagtc tatccctatg
    30421 ctcttcagga tcgcctcacc cacgttcgcg aagagcggtc gtacacggcc ctgaacctcg
    30481 aaaaccagta cgtgaagctg accgtactgc cggagatcgg cgggcggctg tttgccgcga
    30541 ccgataagac caacggctac gatttcttct atcgccagca cgtgatcaag ccggccttta
    30601 tcggaatgct cggagcctgg atctccggcg gtgtcgagtg gtgtgccttt caccatcatc
    30661 ggaacacaac cttcatgccc gtggactact gcctcgcgga ggaccccgac ggcagcaaaa
    30721 cgatctggct tggggaaacc gaacggcggc accggatgag atggctggtc ggcctgacac
    30781 tccacccggg cagatcttac gttgaggcga ctgtcaagct cttcaatcgc acgccgcagc
    30841 cgcattcgat cctctattgg gcgaacgtgg ccgtccatgt caacgaggac taccagatca
    30901 tcttcccgcc cagcgtgcag gtggtgaccc accactcgaa gatcgacttc acgacatggc
    30961 ccatcgcaca gggtcggtac ctcggacgcg attacaccgg cgtcgacttg agctggtgga
    31021 agaacatgcc gaacccaaac tcgttcttcg cgtggaacct gcaagaggac ttcatgggcg
    31081 gttatgatca cggccgacag gcgggcgtgg tgcatgtcgc taatcatcac ctggtatgcg
    31141 gggccaaggt ctgggagtgg ggcaagggcg aagccgctcg tctgtgggac agcatcctga
    31201 ccgacaacga cggtccctac gtcgaactca tggtcggggc ttggtcggac aaccagccgg
    31261 actacagttg gatcaagccg tatgaaacca aggtcatcaa gcaatactgg tatcctgttc
    31321 gccagatcgg cggcttcaag aacgccaacc tgaacggcgc agttaacctg gaactgaagc
    31381 cggacaacca actggcgttc gggtttcacg cgacgacgaa gcacgacaac gccaaggtaa
    31441 tcctgagcgc ggccggcaca actctcttcg agcaaaccgt cgagatagga cccgagaagc
    31501 cttttgcccg ggagatcgcg gttccggcgg gcgtgagccg ggccgatctg cgggcggtgc
    31561 tgatttcggc cgacggtcgg gaactcattg cgtaccaacc ggtccaacgc gagccggtca
    31621 aggagttgcc cgccgtcgtg aaggctcctc cgggacccca ggagatcaag aatatcgagg
    31681 aattatacct gaccggcctg cgaatcgagc agatccataa ccccggcgtc aaccccgacg
    31741 actactaccg cgaggcattg aaacgcgacc cgggcgacac ccgctgcaac accattcttg
    31801 gggttcgcgc caacaagaga ggcttgtacg aggaggctga aaagcacctg cggcgggcaa
    31861 tcgagcgact ttcggcagcg tacacccgcc cgggcgacac tgaggcgtat taccaactcg
    31921 gcttgtcgct gcgcgctcag ggcaggtgtg acgaagcgta cgacaacttc tatcgggcat
    31981 cctgggacca ggcctttcac tcggcggcgt ttaatcagct tgcggagctt tcctgtcgag
    32041 aaggtgcgtt cgtggaagcg agggagcaga tcgagcggtc gctctcgacc aacaccctga
    32101 acaacaaggc ccgcagtctc caatcgctgg ttctgcgaaa gcttggggac tatcaggccg
    32161 ccaaggcgtc atcgtccgca gcgttggcca atgatccgct cgacttcttc gcccgcaacg
    32221 aactgtacct ggccctggcc gctgagaagg cccccaatgc cgcggcggac gtactggtcg
    32281 agctgagacg cctgatgcgg gacgaagtgc aatcttatct cgagctagcc accgactaca
    32341 tgaactgcag ccagtggaac gaggcgatcg aggtgctccg tcggccgatc gagacgaaga
    32401 tgcccttcgc cgcgacctat ccgcttgtgc attactacct cggatacctt tacgagcaga
    32461 aggggaaccc agcgcaggcc catgagtcct acacgcgggc cgcggcaatg ccgagcgact
    32521 actgtttccc ttttcggctg gagacggcgg acgttctcaa cgcagccatt cgggcaaatc
    32581 cctccgatgc ccgagcccac taccatctgg gcaatctgct gtttgatctt cagcctgaag
    32641 cggcgatcgc gtgctgggaa aagtcggccg cgatcgatga tcagttcgcc atggtgcatc
    32701 gtaatctggg atgggcctgc caccgggttc tcaatgacgt cggccgtgcg atcgagtgct
    32761 acgagaaggc cgtcacccgc aacccgatgg atccgcggct gttcttcgag ttggacggtc
    32821 tatacgaaac agcaaacgtg gatcctgcgc gtcgcctggc agtcctggag aagaaccatg
    32881 aggtcgttcg gcagcatcaa gacagcctgc ttcgtgagat catggtgctc gtgctggtcg
    32941 ggcgatacga gcaggccatt gaccacctgt caaagagtca attccacgcc cgcgaggaca
    33001 caggccacgt ttacgaaaca tacgttgatg cccatctgct acgaggcctg acattgctga
    33061 gagtcggcag gaacgtcgag gctctggccg attttcagcg agcggccgag tcgccgaaga
    33121 atctcgcgac gccgcggaac aacctccgcg tccccgagat cgcctaccat acggcaatgg
    33181 cgcgaaaagc tctcggtgaa accgccaagg ccggggaggc cttcgaggaa ggcgcctcgc
    33241 acccaggctc ggcctcgatc ccgcagaccc ggtactgcca cgcgctctgc ctggcggagc
    33301 tgggcaaggc tgacgaggcc gcgaaggtgt tcgacgatct catcaggacc ggcaaggaga
    33361 agctgacgac cagaaccgac gagcccgact tctttgccaa attcggcgag caggaggcga
    33421 ccaacgcgcg cgccgcatcg gctcacctgt ccatcggcct gggcctgctg ggcaagggaa
    33481 aaccctcgga agccaaacag gagttcgagc aagccgtcag gcttaatctg ggcaatgtgt
    33541 gggcggcgta ctacctgcgc ccatagaagc ccctgtcaag ctcctcttgt ttgtctcgcc
    33601 agcgatcatt gcttcggacc aaaggtaacc gcagcgaatc gtcccacctc atgataaccc
    33661 gtgaaggtcg gtgagagggc gatctgctcc gtatgtttcc agcccttttc gcgatcttcc
    33721 aaggactgcg gcatagtgaa cgtcatggcc gttctgtaga cgttaacacc ccacttgctg
    33781 cccggttgcg gctgtgcatt aaccgacttg aacgggatgc gcacctcggc aacccaaaga
    33841 tcctttccct tctggacctt gcattcccat ggttcgttgt acaaggtgaa gttgtagaac
    33901 cgtccctcga tcgtggcacc ggccgtgtta gcagcgaatt gatagtaatc catcttgtcg
    33961 aacctgctgt cgatgaacac ctcaaaggaa tcgtcgccga agatacccgc gctatccggt
    34021 cctcgggcat tggctacgag caggtccatc ctcggctccg tggcctcgat ggccacgtag
    34081 aaagcgtccg agccgtacgc gagccgcacg tacgtgggct tctcggacgg ttcattgtgg
    34141 ttggtcctga gcggaccgat gcgctcggcc ttctgccagc cgggctcgtc caggcgaccg
    34201 tcaacggtga tcccttcgac atagacggcg gtcaactccg gcaccggccc tttctgctgg
    34261 tcacggtacc aaccgggcag gtaaatgggg cgcagcgggc gcggtccttc gggcaaatca
    34321 gccaatcttc gctcggccgc gttgttgccc tcgtagattt ccttcaccag attgcccgtg
    34381 tccacccgca cgcgcacgcc cgttgccttt gggccgaccc gccagggcat cgagaccatc
    34441 acgctgctgg gcaccagggc acggggacaa gccaatgtct cgatgagttg ctctccgatg
    34501 accctgccgg agcccagttc ctcggccagc accttcacct gccgggcgtc ctcagtgccg
    34561 atgttgtgca cccgaattct cagttctgcc tccgtccccg gatctggagc cgccggaacg
    34621 caaaacaggt cgtcgctaca gaccgccaca tccgcccgcg attcccaagc ggggccttca
    34681 gccaactgaa tgaactccat gatatactcg taacccggct ccaggtcgaa atccagaatg
    34741 gagcctcgtt caatgatgac ggtttcatcg cgaaccagcc ggtccggccg atcgtccgca
    34801 ttggcgtcga tcccgaaggt cactcgatat gccccgggct ccaactggaa aagttgagca
    34861 ccgatggagc gaggtttctc gcccacgttg cagcacagtg ccttgaagcg ggtcaggctg
    34921 tgttcgagca tgaccacggc cagctcatag tcgcctcgca gccaccgcac cggtggaaac
    34981 ggcaaatcca caaaatcggg gctggagtgg tcggtaccgt agtacaacag cgggaagtgc
    35041 tcgcccaaca ggtcgatgga cgcttcgtaa ttgtccgtcc cgttcctgat attgatccgc
    35101 ctcgccttct cggcctcaac ccgggcgtac tcaacggctt tcctgccggc ttcgagtgcg
    35161 atctgtctcg tcatacgggg cggcgaaccc caccagcgat cgtactcgtg gtccccagtg
    35221 gcccagcgat aggtgtaaag caattctgca cccgtccgtc cgtagcgggt cttctcgtcc
    35281 ggcggcagcg tgacagaatc ggcgttcgag cgattctccg gaccgagaat gtccttgatc
    35341 gcatatccgt gccgaagcat cccgtacatg cccagttccg tgcgcgccgg gagcagaaag
    35401 tccggatcgc cgctgatcgc atagtcgaga atcagcagct cgagcaagcg gatacgctga
    35461 ttatggagga cgaccgcccc ggtataccag gtgcgcgtga atccgccggc ctcatccgtg
    35521 gcaaacacca cctcgcacgg cagcattccg taaggcttgc cataggccgt ctccttggcg
    35581 tggtcgaccc atgcccggcc ccattccagg caataacgag ccaggtgggg atcgcggttg
    35641 taccacaaca gatgcatcgc aggaatcatc gcgcgggcgc aaataccggc atccgacccg
    35701 aaataggcgt gttcccagat ctcctgggaa ccaaaaaagt tggatcggaa atgacggtgg
    35761 cccctggcgt tgacggcggt ccatttctcg acattcttgc ttgtggtcat catgcgttcg
    35821 atgaacacgg gattgccgta gtcgatgatc gtcatcagcg gttgggtgtt ggacgtcacc
    35881 tcggccgcgt gggccacgtc acgggcaccg gactcataac catccatcag cgactcgttg
    35941 taccacaccc agtcgagggc cttgcgcagc gattcgataa tccgctgatc gtccgccgcc
    36001 atgacgacaa tcggccacat ctcgtagaac tcgcagtcat cgttaagccc gaacccgaag
    36061 ctgccgtcct cggcttggcg atcgatccag taatagatca cctccgtcca ggccttgagc
    36121 ccgcgacatt ccataaccgc ccattcgggc ggagcaacgg cctcggccgg ggcagaaagc
    36181 aatccgagca gaattaacga ctggcaaaaa agacaagtcc tcagcattga atcactcctc
    36241 atcaggacag acaaaaacgt ctttacaagc ggcttccttc gggtatcgtc tcgaacggga
    36301 tgctcggtct cacaaaggaa tgaggttcac tgcaaggatc gcaaaccctc ccggccatcc
    36361 agtcgatccg cggcatcagc gcgcactttc ggcgcatgga taccgacctt ccagatgcaa
    36421 cgaggggcaa ccgcaactct tattattgct catgacaatc ctcgggtcaa ctcggggcat
    36481 cgaccgatgc gcaccggttc tggacggcct tgcgactgtc tgttttcgag gctttggtgt
    36541 ggtatgcttg ttcgctgaca ggattggtgg aagcgatgcg ggaccggcgg caaaacgtga
    36601 acccggagca cttactgtcg gagaatacac aaccatgatc ggaagaaccg tgtctatgtt
    36661 tgtcgtcgtg tgtgcttgcg tgatcttgtt ccacgcggtc agcggatgcg gtacttctca
    36721 aggcgcctcg tcttcgtcct tcactttcac cgtaaccgcg gacatcgagg gtgccgcttt
    36781 tacggctccc gacgaattgc gtcttctttt tcgggacatc aaggacacgg gtggaccggg
    36841 tgcattcatg attcatgtgg gcgaccatat gccgggaatc gaagcccagg acattgagct
    36901 gaagaaggcc tttggggccg acttcccctg gtactgcgtg ctgggcaaca acgaccagaa
    36961 agagcccgag aaatcgatgc ggttcatccg cagtcacctt cgtgagttgc cgtacatcgt
    37021 caacgtcggg cctttcaaca gcgagacgac cacgtactca ttcgactacg gcgacgcaca
    37081 ctttgttgtc ttgaatcttt actacgacgg gtgcacggac cacggtacgc gacggccggg
    37141 cacggccggc aaggtcatcc ctcagctcta ctactggctg gccgacgacc ttgccgccac
    37201 cgacaagaaa tggattttcg tcgcagccca tgaacccgcc ttccctcagc cggacgccga
    37261 ttggggcgac gtccggcatg agaacgacag tctgaacgag taccctgccc agcgtgatgc
    37321 cctctgggaa ctgctcagcg accaccgcgt ggtggcatac ttcaacgggc acatacatcg
    37381 ttatgcgcgg tatctcaaag acggtgtctg gcaggtcagc gttgcacagt cgcgaggcaa
    37441 ctccaagtac gacagctacg tccgcgtcca cgtcgggcgc agcgaagtga ccttcgatgt
    37501 ctatcgccct ctgcacgaag gcgtctttcg aaagacggac acatgggaag tcacaaggcc
    37561 cgacgggccg acggcctttc cgcacgccat ggccgacccg gacgcggcgt gggttgggat
    37621 cgaatacgtc aaacaactgt cactgaccca gcgatgcccg gcgaccgact ggtcggtgat
    37681 tgagggcccg cccgggctca ccgttgaccg gcatgggttc gttcgtggct ggactccaac
    37741 agcgcagcac gtcggcagcg atcggagcat caccgtccga gcggccaaca ctaggggcgt
    37801 ggccgaggtg acgtggcccg tccgtgtttc gccgctaccg gccgataccg tcgccatgtt
    37861 cccgttcaac accggtccgg aaggttggag actggagact tggatgggcg gcaagtatgg
    37921 gccggcgagc atatcctggc aggttcttgg cgggcatccg ggcggaaccg ttttctcggt
    37981 cggtcacggt gccaatgatg acaaccgctg cacgcgagaa gggacgatcc tgacgcgagc
    38041 catctccacc agaggattcc gagacatccg agttgagttt gatgtgattg gcgatctggc
    38101 cgccccgccg tgcggacaaa ccgacggcgt tgagggcggc gtcgacggct ccagcgaaga
    38161 tcagctcggc gtctgctaca gcaccacggg accagagggg ccgtggacaa ccgcccgcgt
    38221 cttcaaggcc ggtgacctgc ccgccgaatg gactcgaaaa gtggtcgatc tctcaacagc
    38281 tcaaggaacg gcagacaacc ccgacttcgc gctgcggctt gtgtggcaat tcaacacctt
    38341 cagcgactcg ggccgcatcg acaacgtggc cgtcctgggt gctcgttgaa tcacgccgat
    38401 gcgggttcgt cttacgcatc cgcctgggcc agaaccgacg tcgagacagc cgctttccgc
    38461 acagggacgg cgggctgtgg cgggcgagtg tgccgcctga tcgggcctcg cttcagacac
    38521 aatgcccagc caagcgtgcg ccgaacggtc tgaatggtgc cccacattcc gagaacctgc
    38581 atcatagcat ccgcgagata aagcctggtc ctgggccgga tgattgcccg gagaaagcgc
    38641 agcggattgt agaagtagag gtacgccagc atgatgttca actgtttgcg ccagggccgc
    38701 gggtcccggg atgccaccac atagttgccg tccagcatgt gctgctcgac ctggcgaccg
    38761 gctgcgatag cgtacgtcag gccggaagtg aagctttctt catatcccct ggatcccgtt
    38821 gccggggtaa tcatcaacac ctgcaggccg gcagccccgg ctcggcgaag caaacgcacc
    38881 tggttcagca ggccgcaggg gctgccgcgc gtatacaagg gctggccatc gtggtgcatc
    38941 atcatgggca tcggaacaat gccgtgtctc gataagagac gaaacacctc ttcggtccca
    39001 tcgacgctct ggcccttccg aatcagtgcg cccgtcatat cttccacacc aagccacagg
    39061 gcccttatcc cgcctgcccg aacaagtgag aggtgatccc gcatttgcag ggtgtcgtga
    39121 accgttactt cggtcgccca gcggaccgtc ttgcggaact tgcctccgtt gatcgcactg
    39181 cgggccagag tctcgacaat tcccagcgtc cgttgcttgt tgttgaagaa gttgtcgtcc
    39241 gtcccgaaga aatagcggat gccatattcc tggtgcagcc ggatgaactc gtcggcaatg
    39301 cgcgccccgc tcttggcccg atgctgtcgt tggttatatg ccggtatggg gcaataggga
    39361 cagttgaact tgcagccgaa ggtcagcacc agcgacgcca gcgggctgta cttccgcacg
    39421 cggccggcct gcaggctctc ggcggccagg gtggcctcgc ggctcggcgg ctcaagcagt
    39481 cgatacccca aaaccgggtg cggcagctcg tcgaggtcgc tcagcagccg ttgtgtgccg
    39541 gtgtcgacaa gctcttcggc tacgccgtct ttctcaccca aagagtacac cagcccgggg
    39601 atcccatcga gcatgccgcc gtcacgggct cgtcgaaagg ctgaccgcaa cggttcgcct
    39661 tccgctcgca cgctcagcag gacctccatt agactcagca gcacatattc ctcgccggtc
    39721 acggccacgt cggcacccca gggctgccga ggatcagaac tgaacacatc ccatggttcg
    39781 taaatgacct tcgggcctcc ggcgatgatc aagggtctct tggaagggtc gatacggcag
    39841 gcgtcgcgaa tcagggcatg gcactgagcg gtgtgaatct gcatgctcga aaccatgaag
    39901 agatcgggaa cgcgaccgtc gagccgcatc aggctgggct tgaagttggg attccactgt
    39961 tgcagcacga tacgggtctt cgaaaacccg acgtccgcca tcgccgagcc gatcgccctc
    40021 acgccggcgg gagccatgcg ggtgtcggcg taaataaaag ggagcattcg tgtgcgatgg
    40081 tcgaaggcgc aggcgatcac gcttgccagg tcgtgcttgc ccgagattcg gcgcagggat
    40141 tctcgcaggc cggtcatctg gcccggcttg agcaactcat cgccgcgtgc ccgacgcggt
    40201 atctccatga caaagcaact ccgctgatca ggcgaaccgg gctccggttg tcgccggacg
    40261 tcgtgtcgtc tttcgacctc aattgccgac gcaaacagga taaacgcagg cgaggagtct
    40321 gtcgagttga ggcaatgcag gcgaagtcga cgattctcat gccgtctccg ataaccactc
    40381 atgcattggc gtacgagctt gggtcggggc gagtagaccc ctcgacgcgt ctcaagaaaa
    40441 gccgccaaag ccacccgccc ggcgccggaa tcatgcgttg gatcgctcgc catcagccgt
    40501 cccgattacg gcacaggggt gtcgaggcga aactcagctc ctccggcgtc gcattcaact
    40561 catcaaacgt gaacggccga ccgcattccg ggcaacgttg ctcggtcagg ccgtagaggt
    40621 agtaatcaca acccgggcag cggcccttcg ggtcgtaaaa gggtgccagt cggcaggtca
    40681 acaactgtcc gaacgtgcgc agccaggact ggtcggtgag cacggccgcc tcgcccaccg
    40741 aggccaggac gatcagcagg catgcagcca acgacaagta aagaccgtat agcgggttgc
    40801 tgaagatcgg ccagtaactg aaccaggcta accccgacaa accgccaagg aaccagaggc
    40861 agagacgacg ctggtgccga cggcggatcg cgaagatcgt tcctgccagc aaaagggggg
    40921 aacacaacag gccgaacacc agattgacat aatcctgagt cgtccacgaa gagggggccc
    40981 acccattcgt gaatccttgc cgtagtgtgg acgccagcac gacaacggct gcgtaggcca
    41041 gcagaatgat gaccgccagc gtgcttgcgc ttcggagctt gggccaggcg acgagcgctg
    41101 ccagggcacc gatcatcagg gcggcgccga acgcgtaggc cacaaaatac acgacgacaa
    41161 cggcgacaac gtcgattagc cacgaaccgt tccaagcgtc actcagctcc cagaccagcg
    41221 acgccgcagg gacgatcggt ccattgcaac cctgaacggc cggtagaaga aaacaagcgg
    41281 ccaggagtaa acccgagaac aagctcagca gccgccattt ccgagcccgg cggtcgggtc
    41341 tgtattcacc ggacggcttg gacatgtcgg aacagcttca accacgtcgg tctccaggtc
    41401 aagccgctgc gagtttttca gggttttctc gaaaccgcac cccactgttg accggggcgg
    41461 tcgtccccgt cccggcgtag cctgcccgcg aagccggaag ggagcgactt cttcggggtt
    41521 tggccctctt gtggtccggc tcctggtctg tgctccggtt tcggcaaggg ccgcccaaag
    41581 tgccacggtc ggccgcatca cattccggta gctgtgcgct tgatcttgaa gccagggccg
    41641 tgcaagatgg ccccaccatg gcatggccgg gacagcagcc tgtccccaga cccgtgtggg
    41701 accggctgcc ggccggtctg agtgggggcg tatgggacat gagctccaat tgagcaagaa
    41761 cccttccgac agagaggatg tcagtcatga tcaaatcgat gctgcgcgta atcgtctgtg
    41821 gcgcattcgc cgcatgtgtt gccgccatcc taggtgccga taccggtccg gaacccaagc
    41881 cgccagtccg gaccaacaaa ctccgcgtgc tcatcgtcac cggcgggcat gacttccagc
    41941 ggaaagagtt cttcgagatg ttcgacggcc atccggacgt cgcctgggaa gaggtccagc
    42001 agcccgaggc ccagaagtgg ttcgccccgg acaaggccga cagctacgat gtgatggtct
    42061 ggtacgacat gtactcgcag atcaccgagg aatcccgcaa gaaccttgag gccttgttga
    42121 agaagggtaa gccgctggtg gccatgcacc atgctgcagg ggactaccag gactggcccg
    42181 aagcgatcaa gatcatcggg gcaaagttca acctcaagcc cggccccacg gaacgaaagc
    42241 cctcgttcaa gcatgacgtc aaggtgcggg tgaagatcgc tgacccgcaa catcccatca
    42301 cccgtttcat gaaggacttc gagatcgagg atgaaacata taaggacatg gagttcctcg
    42361 aaggtaccca cccattgctg accaccgacg agccgacgag cgacaaggta attgcctgga
    42421 cgcacaccta tcacaagagc ccggtggtct acattcagct cggacacgac gccaaggcat
    42481 acaccaaccc gaaccttcgt cgactggtcg tgcagtcgat ccggtttgtc gccggccgtc
    42541 tgcccgatcc gtcaacggag ggtttcgttc aactccttaa cggcacagac tttacgggtt
    42601 ggacgatcgt cggcgatccc aagggcttct ggttcaccga cggcgttctg cgaagcgaga
    42661 gcggcaaagg cggcttgtgg atgcggacga acaagattta tcgcgatttc atcctgcggg
    42721 tcgagtggcg gatcggtaag gacggcaact cgggcgtgtt cgtgagagcc gcggcagagg
    42781 gctatccttg ggaaaccggc agcgagatcc agatcagcaa tgagcctcgc gatatcgccc
    42841 actgtaccgg gtcgctctac ggaacggtgg ccgtcgatcc gcgaccggac gagactgccg
    42901 acgtctggca catgttcgag attcactgcc gcggatacca ctacaaggtc ttcgcggaca
    42961 acatccccgt cgtcgacgtc gatgccaaga ccatcccggc cctggccgcc aagccgctgg
    43021 agggctatat cggcgtccag gactcccaca accgaacatc atacgtggaa taccgtggca
    43081 tatgggtcaa ggagctcaca ccgatgagta gtgacaagga gccggcctgg cgcgtcggaa
    43141 cgcaggcgta cacattcaac cggttcacct tcttcgaggc cgtcgacaag gccagttcga
    43201 tcgggctgaa gtacatcgaa gcctaccccg gccaaaggct gagccccggc cttggcagcg
    43261 ccaagttcga tcatgacatg gcccccgacc tgaggcagac gaccaagacc aagctggccg
    43321 aggcgggtat cagactggtc aattacggcg tggtcggcct tggcaatgac gaagccgcag
    43381 cccgcaaggt gtttgagttc gccaaggata tgggcatcga aacaatcgtg tccgagccgg
    43441 agccgggcag cttccagatg ctcgacaagc tcactcagga gtatgaaatc aacgtggcta
    43501 ttcacaatca tcccaagccg tcacggtact ggaacccgga cacggtgctg gacgcgatca
    43561 agggaacgaa ctccagaatc ggggcctgcg ccgacaccgg ccattggctt cggtccgggc
    43621 ttgacccgat cgagtgcctc aagaagctcg aaggccggat catctgcctg catctgaaag
    43681 atctgaacga aaaggcgatg accgcccacg acgtccactg gggcgagggc gtggcgaaca
    43741 tgaagggcat cctagccgaa ctcaaacggc agggcttcaa gggcgtcttc tctgccgaat
    43801 acgagcacaa ctgggagaac aacgcccccg acgtggcggc ttgtgtccag aatttcgaga
    43861 aaatggcgaa ggaactggga atgaactgaa cagttcatcc ggtgtcccgg gactgacggg
    43921 ctcacaggtg gcgcccgcct ggagtctacg gctttgtcga gcggcaagcc atgtccctca
    43981 ctgaggccga ggatcgccca tgctgtaagc tgccccacat cagcttggtt aacggcggcg
    44041 gtgtctggct gctgcaaccg ggccatctcg tggcttagcc gccggaatcc cggccttgcc
    44101 cactcctcag aagggggccg tgtcttgatg ggagtttgcg atcggagatg agtctgctga
    44161 ccggcgcatc gtgtaacccc cagaaaccac gggggccgct gatttgcggg cccagggcag
    44221 gcgctccggc cttccttccg ccgcagattt catccaccaa agctgtaata ttccaggtcg
    44281 tactgctgag ggtcgctcgg ggcagagctg cgggatttcc gcaattccat cttccaatta
    44341 ggcgcgggtc ggttacaatg cacgagttgc ttcccccttt ggtgccgttc tgctgaaagg
    44401 cggaacggca agaagctagt cgcaaggact ggcaataccc ctaccttacg gtcctggttc
    44461 accccggccg ggaccatgag tgccggaacc tgcgatagct cgccaatggc cccggttccc
    44521 cctaccgggg cctgtttttt tccgccctat ggaagtattg aaaacagaat cgcaagtcat
    44581 ggcatcgcat ggcgatacag cattttcggc ttcggtcgct ttttcgtcgt tcgttgcgtt
    44641 tccatgccct tcacgcatgt tcagtacaac gctggggaca acacccacta caacgcttcc
    44701 atcgtcgtaa gtcccgtttt tctgttgggc ttgggctgat tctcgggccg aagttcgggc
    44761 agtcgttcaa ccgccccgaa ggtgtccaag agctgcggat cggtgtaggt ttgcatggtc
    44821 aacttcaaca cggccggcaa cgcctgtttg ttgcgtttcg ctacgctgat cgtcatgcct
    44881 ttctgccccg cctgcgcgtt gccgtggttg agacgctaag gacgtgaaca ccgacctcgt
    44941 cggcggcgtc gccagtggct gagcgccacg gccttgcgcc gctttgatat tcggcgccat
    45001 cgccgttggc ggagttgcgg gagggtgaca agcatgagct ggctgaacat ggatggctga
    45061 ggagcaaaga cctcgccggc gttgctgaag ccccatacgt cgggatggga atcaaaaccg
    45121 aggctttcga catcgttttc tctgtcggat cagacccgca ttcctgccgt gtccgaagct
    45181 gctttctgct gcgatcccga cgcagcaacg ttatctcgcc tgagctctga agcacctcgc
    45241 gagcctgcag ttatccggaa ggtccgatcc ccacctggtt atgggacccc gagggttgcg
    45301 ggccggtctc cggcccaacg accccggctt ggccacgact ggcccagggg ctgttctggc
    45361 caaggcttgc cccccacagg ggcaattacg gtgtggcgtc cggaaatgtt gcatcgggtt
    45421 atcccccgcc cgtgccagat ggttcggggc accggcctgc ccgaaaacga ccgcctcgag
    45481 gcagaagctg gagacttggg cacagcgtct cggggaaggg ttgacagctt caggggcctt
    45541 ccggcgagga aaccccccat cgggcgcgtc cgatagcgaa acgggaatcc gaaatccgca
    45601 aaaatcgacc gaaaaatcgc aactgctcgg gaaagacagc agctcccgtg cataggattt
    45661 catgagaagg cttccgcttc ggcggagggg gtgtacagag agtcggggag agggcgggct
    45721 gtgccggtcg gccaacacgc tccaatcgga gtccccattc acaagtcggt ttgtttcgtg
    45781 ggccggtccg tacggcggag atgagacatg gcgtgcgacg gctacttgcc tttgtggaac
    45841 cgtgttctgc tggacgtgtg cacccagcgg gattttctca cgccgggttg tgtgttgcag
    45901 gttgccaatc tcgattcgct gatcgcgaac ctgcaacgcg tcttcggctg ggtgcgatcg
    45961 aacggtattg ccgtggtttc gctggtggaa tcgcaccggc cgaccgagcc gatgcgcgaa
    46021 ttcccgctgc attgcatcga taacacgccc ggacaggaga agctgccctt cacactgctg
    46081 tcaccccgca ccctggtcga aacagacaac tacttgtctc tgccgccgga cctccaagcc
    46141 aacaaccgtc aactcatctt ccggaagcga acacgtgata ttctcagcaa tccaaaggcc
    46201 gaccgattcc tgacctcgtg gcaaccggag gaatacatca tcgttggcgt gggcctcgaa
    46261 cgggcgattc gtgggttggc gctgggcctg ttggcccgcc acaaaacggt caccgtggtc
    46321 accgacgcgt gcggctgctg gagcccggcc gacgccgaat tggtcatccg ccagttggga
    46381 gccaaggaca tcaggctgac taccgtcgaa gagctgaccc ggcctccggt ggtcgtggcc
    46441 aagcggacct tgcttcgtct gcgtcgaagt cgacgccacc atccggtcgg ctctcgaccg
    46501 gccaaactat cttcccgggc caaggtggcc aacggctgat cctgcccgag aattcaatct
    46561 ttactgtatc atctacaacc cttcttgctg cagccctgat cgcgttcgcg aagcattcgg
    46621 ccgactcgtt ccctcggtgt caccgtcgcc agtttcattg gtgcgggttc gggctatcgc
    46681 gcacattgta tggcaagctg ccaatgaccc tccctttcaa aggccgtgcg cgatggtcct
    46741 acatagcggt cgacatcttg acttacagct acccatatgt aggatctcgc attggcggct
    46801 gccggccacg gccggcactt tcacaggaga ctgctatgtc cagcctcgac gacccgtctc
    46861 aagggttgcc caagacaaag caactcccgg tgaaacggga ggtgaaggag ctttcgctgc
    46921 tctttgagat cagccagatg ctggaccgca gcctcgattt gcgtgaggtt gtccacccga
    46981 tcctgcaact tctgcatgag cggatgggca tgtctcgcgg aacactgact ctgctggatc
    47041 gcgagacagg gcaagcgcac atcgaggccg cccatggcct gtccgccagt cagtgcagac
    47101 ggggaatgta cgcccccggc gaggggatca ccggcaaggt gatcgaaagc ggccggctca
    47161 tggtggtgcc tcgggtctcg gaggagcctc gattcctcga ccgaacgcaa tcccgcaagc
    47221 acctcaggaa gcaggacatc tccttcatct gcgtcccgat caagctggga aacgaggtca
    47281 tcggggcgct gagcgtcgac cgcttgtttt ccgaagaggt ccggcttgac gaggacgttc
    47341 ggctgctttc gattatcgca tccatgatcg cgcaggcggt gcggttgcgt cagtcggccc
    47401 aagccgagcg tcagcggctg atcgaagata accttcggct ccaggacgag ctcaaggaac
    47461 ggttccggcc ggccaacatc atcggcaact cacgggtcat gcaggccgtg tacgaccaga
    47521 ttgcccgggt atccaagagc gacgcgacgg tgttgatctg cggcgagagc ggaacgggca
    47581 aggagctggt cgccaacgcc attcattaca acagcccgcg ggccggcaaa ccgtttgtca
    47641 aggtcaattg cgccgcgctg cccgagacga tattggaaag cgagctgttc ggccacgaaa
    47701 aaggggcgtt caccggggcg gtcgcccaac gggtcggccg atttgagatg gcctcggggg
    47761 gaaccatctt tctcgatgaa atcggggatt tctcgatggc cacgcaggtt aagctgctcc
    47821 gcttcctgca ggagcgcgaa ttcgagagag tcggcggaac caagaccatc cgcgtggacg
    47881 tgagggtgat caccgcgaca aaccgcaacc ttgagcaact catcgccgag aataagttcc
    47941 ggcaggacct gtactaccgc ctgaacgtct ttcccattca cctcccgccg ctacgcgcgc
    48001 gcaagtcgga cattctgctg cttgcggatc acttcgtgct gaagtacggc agtctgaacc
    48061 acaagaagat caagaggatt tcaacgccgg ccatcgacat gctgatggcc tatcactggc
    48121 ccggcaacgt ccgcgagctg gagaactgca tcgagcgcgc ggtactggtc agcgacgacg
    48181 aggtgattca cgggcaccac ttgcctccaa gcctgcagac cgccgaggcg tcgcacacca
    48241 atctggtggg cacgctgcag gtggcgctgg acaaccttga acgggaactg attgtggacg
    48301 cgctgaaatc ctcccggggc aacgccgcca agg
//
LOCUS       DAOTPW010000006        48305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_805_length_48305_cov_5.499233, whole genome shotgun sequence.
ACCESSION   DAOTPW010000006 DAOTPW010000000
VERSION     DAOTPW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 48305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48305
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_805_length_48305_cov_5.499233"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="P5316_00955"
     CDS             complement(<1..1431)
                     /locus_tag="P5316_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83550.1"
                     /translation="MKTKVGVPILKGPGFIAKLRQIACDPLPVDPWPDLKYRNVLIAG
                     VVGGEVHFSRMGIIGQGLRMRGATVTLLQCDEFLPACTMRKVDHYESACTRWCHKNSG
                     PFARAIGLPCRWYSEFITPAERKECDRIAADVDLHALHAFEHRGVPLGEHVRQSIESF
                     YKIGYFDANDPETAGMARQFVRAALYMTTVGFRALEQLKVDKVIEEDGKKVDWGVIRG
                     VAGRMGIPVDVIRSGLGYYSIRFEHDRPPNRSAMMPEWDTWKHIPLTPEQEKALDQYM
                     LRRERVPYEYRSADWQTNTTDLREARRHAGLPDRIAGKVFGMFPNLSYDADRNKTVAA
                     FESANDWITETIRFFCRRPEHHLVVKIHPSEKLWKARDPLLPQIEREFPSLPPNIHVL
                     PPESDVSAHAVARLSDWALVYTSTVAAEAAALGKHVMLVGGGWNAGRGFSHDVTSARD
                     YFEKLEGICSGAWNPEGSDLQCGRGGP"
     gene            complement(1656..4328)
                     /locus_tag="P5316_00960"
     CDS             complement(1656..4328)
                     /locus_tag="P5316_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRR83551.1"
                     /translation="MSVIETMPATIPQRLAKNAETPDCAEFLCLLHADGEPERFSYRD
                     VLNEAGRYTSFYRQAGLVPGDRMVIILRHSLDAYASFIGAILGGFVPSFWSCPSPKIS
                     AHEYLKMLASLLAGAKPRLLLTYSRLGDQPEALTMFRDAAVPSYVVDEVPKTTEAPAW
                     HMPDDPETIAFLQYSSGTTGAKKGVAISHRALLWQVDHYADAIRLDSSDRIVSWLPLY
                     HDMGLIACFMLPFIRQVPLVAMCPFEWVQRPAMWLQAISDFRGTLSWLPNFAYSFMAT
                     TVTDTELAGIDLSSLRGIVNCSEPLLSRSHEQFVRRFAACGLSNTALCSSYAMAENTF
                     AVTSGGFDGPLADDCIDGPTFAGTGQAIPAPADSPTCRRMISSGTPLPQTEITIVDDQ
                     GRPLPDRRLGEIVLRTPCLLTRYDNNPEATAKALRDGAYFTGDLGYVADGQLYVTGRK
                     NDMLIIRGQNIYPQDIEEIVSATPGIIPGRCAAIGVPNERSGTEDLIILAETQETDPE
                     KRESIRRDVLARIARATEVAPADVCLLEHMWLRKSTSGKISRNVNRDRYIEMIADSRS
                     PESASCSPNAATTAESTLSAVRRCVTQVLSQVRAGRFARVGDDEPLVTSGLIDSLGLV
                     SLLTAVETGFRVQIPTPILADVLNIDTIRRIADLVDRLRTGAPGPKDFDSFPHGPDDV
                     PLLIGAPRTTRRTKGFWAHYYRWVFRRHGIRVGKGLRVLGPLMLRFDGDPRNITIGED
                     VTLMPGVDLKIRENGSIILHDGVVLDTNVRLVAARDGRIILGENAQLGMGTIVNAGAD
                     VIIGRRTAFASYCTIISSEHNYLTREPFMRQGYRHEPVYIGEDVWGAAYTFVGRGARI
                     GNGAVIGANTQIRGHIPAWAVVMGNPARVVRFRT"
     gene            complement(4315..4659)
                     /locus_tag="P5316_00965"
     CDS             complement(4315..4659)
                     /locus_tag="P5316_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83552.1"
                     /translation="MKFEENNPPRKFSVGRTGAIEISDCGRVFLDPDEMVTFVTPRGH
                     EYDVTAKDWGFYATPSVNARLRDQGFKTALVRNQQGRYYIMLVEKDRMERFLMYARDH
                     QQELCEWLDERH"
     gene            complement(4656..5774)
                     /locus_tag="P5316_00970"
     CDS             complement(4656..5774)
                     /locus_tag="P5316_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HRR83553.1"
                     /translation="MKYCTRCVLPDTRPGIVLDSEGVCNACKSVAAKREIDWAGRKQR
                     LAEIISEAKRRSLGYDCVIPVSGGKDSTWQTIVCLEYGLRILAVTWRTPGRTEIGQRN
                     LDNLIRLGVDHIDFTIHPEVERRFTLKALRKTGSSAVPMHMALYAIPLKIALAMDIPM
                     VVWGESPYMEYGGTDREIEIDRWDPDTLRRHGILQGTTAEDWVDEELTLKDLEPYRLP
                     PQEQFSGKRIMSIFLGYYLPWDPWESKRVATARGFQADARGPRVGLYDYADIDCHFIS
                     VHHHFKWFKFGFTRLWDNLAIEIRNGRLTREEAVRIIAERGDQTPHDDIQGFCKYLGI
                     TVDEFRQIEDRFRNPAIWTREDGRWVIKDFLIKEWKWT"
     gene            complement(5771..7654)
                     /gene="asnB"
                     /locus_tag="P5316_00975"
     CDS             complement(5771..7654)
                     /gene="asnB"
                     /locus_tag="P5316_00975"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRR83554.1"
                     /translation="MCGIAGYIGRSEIGPDRIDRTLALMRNRGPDHQAHTVFRDGDLH
                     VVLLHSRLSILDLDPRSNQPFSIGDHHLVFNGEIYNYVELREDLERRGISFNTESDTE
                     VLLQSYLAYGPGCVEKFEGMWSFAIYDRGEHRIFLSRDRFAEKPLYYIADDDGVYFGS
                     EVKFLQALADRPLAINQRQVLRYLVNGYKSLYKQPETFFTEVREVPYASNLEIRDDLR
                     PRLQRYWVPQYTPRDMTLGEAVEGFRHYLQESIRIRLRADVPLAFCLSGGIDSGAIAS
                     IAAKVFNYDVTTFSISDEDERYNEFDNIRATVDDLGCRHHVMSIPHRDMRRRLEELVR
                     YHDAPVYTLTYCIHSCLSEMISRGGFRVAVSGTAADELVTGYYDHFNLHLYEVRHHPD
                     FATWLADWHDGPGRFVRNPHLKNPGLYFDNPDFRDHIYRNNHVFAEMLTCDFREAFFE
                     ERFCDSLLRNRMLNELFHEATPVILHEDDLNSMYYSVENRSPYLDSRLFQFAFSIPPE
                     HLIRDGYGKYVLREAVKGILNDKVRLDRQKKGFNASFHALFDPRDPEDRDFLLSEGPI
                     FDLIRRDRIEALLGEETLSNSFSKFLFNFVSAKLFLEQWARGTPGSVTPDRDTALCGS
                     TQS"
     gene            8007..14783
                     /locus_tag="P5316_00980"
     CDS             8007..14783
                     /locus_tag="P5316_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HRR83555.1"
                     /translation="MTNSKDIAAAVLLSLGCGAGAVLAAPVDRPRAAVTAQGWWSLRA
                     QTTLKGRGLPADGPGALKSLRSALSSKTAFAVEQGRSLLDEQGVVRAYQFDLPGGGAV
                     VIAADDAVSPVFFYSLDGKFDPAVPPAAAIWDEFCRQVKENASRPDGNAPHTSWRVIE
                     QSFAGRLDPAFTAAANPIPDVGSSAISRGPLLRTRWHQDEPYNLLAPERLDCTLPAHY
                     CRCPIGCVATSLAQILAFWQSPLFGTGGPAGGECYTWNNGDPESSRWPSLCFDRSWYD
                     GRYLPVCDDADAPFYDWHNMSETVSATDPSVMQDAVAKLCYHCAVSLKTGFCSGGEQL
                     SGAISDPAVPVRYFGFAAGGCLISKSGFSEDHWFEEIKRQIDLGWPLWYDVPNHSIVV
                     DGYAEDIVGGTSERRFHVNMGWGGASNGWYLIGSMPRMPGYEGAVVNLRPAGFGGGPR
                     IREVDADGRSGEYATIQAAINASTDGDEIVLRPGVYTGWGNRDLDFWGKAITVRSVNP
                     DDPAVVAATIIDCQGVQSRPRRAFLFHSGETTGSTVAGLTITGGYAPTHLIGDQIVSV
                     GGAVLCAGASPTIRQCVIRANSAGLAGGGVFSFGEAGPVIQRCVIAGNSAAGGGAVAA
                     WLDSSPKLESCLLTGNTASEGAALHYWEAGRAEVVNCTLSGNRLTDDDGRIVVCQGGD
                     VALTNSLLWNETTGGAREIAVRNAQSSSRVLVSHTDVRGGQAAVVVESGAVLDWAAGN
                     IEGNPGFISASGADGDPLTWHDNDYRLLDGSPCVEAGDNLVLAGETAKDLDGNPRRAG
                     SNVDMGAYEFGSFADCDADGIPDRDQQDRDHDTRIDACDNCPDSPNAAQEDLDSDGVG
                     DACDPDIDDDGVSNGNDNCPRTGNADQQDTDTDGVGDACDNCPLVSNPDQADADGDGT
                     GDECAAARLYVDARASTHGDGRSWSTAFASLEKALSSAAASAGRTTEIWVAAGVYRPS
                     RRIVPEIDGSATFVIPPRVGVYGGFAGSETAREERRPLLNRTVLSGDLKANDVPGSGR
                     SGPSRRDNCHHVVTIEGGDATTVLDGFVVTAGNSMTRGGGMVILDGAPIIADCRFIDN
                     QSSDGGGIYIIGAAPRLTNCLFLGNRAVNGGGVYIGSGDPILSGCVFSGNSSSFGAGG
                     LFCGLGRTTLLNCTVIGNSTSAYGGGAYIASGAAANVTNCIFWGNMRQGAASAKSQLE
                     VAGTATVAHCAIQGSQGLWEDQNNTWRNPMLVDPDGPDDVPGTEDDDWHLLASSPCVD
                     AGTPLAPDAVTPLDVDGEARVRNCRIDIGADESAFHRDCNGNAIPDACEVLDGTGSDC
                     DANGVLDSCEVFSRSRLLVTSAWGDKVLSFDGTTGDYLGPLVDRRAADLREPRAIAID
                     SDRRVYVAAAGSDRVVEYAGNNGLPVRSFAGPDLRRPTALLLVEPTLMLVANGQDNSV
                     VQFDLDSGRPLGILVQSGEGGLAGPSAMLRSPEGHLLVASQQTNRVLEYDWKTGAFRR
                     VVCEGAGLSGPSSLLLEKGRNLLVASRDTGEILRYAMDGTFLGWFVPSGSGGLTRPES
                     MVWGPNGSLFVCNLNTHSVLEFRGLDGSPVDHDPGRPGVQAAFAVGVGSLRPTGLAFL
                     YANECNGNGVPEKCDIASGASTDCNENKWPDECEGDSDGDGVINGCDEDDDNDGIPDD
                     GNRSGYVGDKPCTGGVLTKCDDNCPYDPNPDQADRDGDGRGDACDVTVFVDSRATGAN
                     DGSDWANAFTDLQDALAAAADSDGRVDEIWVAEGVYRPDRGTGDRSSTFMLVPGVRIY
                     GGFAGGEVSVEQRRPLLHPTVLSGDLAANDTSAFEPARAIDNCYHVVTSPSAVLRGRP
                     ALLDGFVITGGWADGHPPNDRGGGLLVVNSGPTVACCEFRMNYARKSGGAVFSGYYGN
                     PAIANCSFLINHAGEGGGLCTVFESNAQITNCLLAGNSAGQFGGAIHSEDSSPTIVNC
                     TIAANHARLRGGGISIDRGNPMVANTIVWGNSLTASSGAGSQIFSPKGGEFVAYSCVQ
                     DDVPGDGRTYRGVQNIDMDPLFVRPAGDGGDGWGIGGNDDPGNLHLRSGSPCIDRANT
                     LMLTENMSGLSGSGVMSGQVLLDLAGFERIVDAPDSESPASFPGATLDLGALEKHPDC
                     NANGIPDACDASCGTPYGPCDVPGCGTSQDCTADRIPDECQPDTDDDGLADVCEWSYG
                     DFDLDGDVDQSDYGLLQRCLGDPASVPLDPSCRGVDLDRDGKVNRSDILVFVRCLVGP
                     DCPADPNCLK"
     gene            complement(14843..15880)
                     /locus_tag="P5316_00985"
     CDS             complement(14843..15880)
                     /locus_tag="P5316_00985"
                     /inference="COORDINATES: protein motif:HMM:NF040416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83556.1"
                     /translation="MSAASQVVCDQAVFTSIRTPTGQGYRIVAASRGVRADEKAEITA
                     RSPSHGSLYESRPDAVGLLSYRLTSRRFCVGYVCHAGVEHTGRGGQRVYTHMALLDEA
                     AYRSFDSNPGAVHAAIGKVIGEAGPLLKPPPHLEPLVLPPGTRDLASPEAVGWIWPAA
                     AMLLEGRRLVLTGESKPLAMLNWIMLSLARSLREHVNASTDLRFSPSRGMHLVLIEGQ
                     DPQLLRQLPGSQMQAWACDSTPPEVPAQFQAWFGLLRRWHREGRFEDIARLTCETCAD
                     SAVESLGRVAAICEDADRLTVADPEMVDEIARRYTSGCAEGTAEQTLVRQLLSRTEQA
                     RSCLHQTAEVS"
     gene            complement(15905..16789)
                     /locus_tag="P5316_00990"
     CDS             complement(15905..16789)
                     /locus_tag="P5316_00990"
                     /inference="COORDINATES: protein motif:HMM:NF040097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83557.1"
                     /translation="MKGQGTTTIAPNRCMICEEINAAQTPQCLSCAAPMALVHDSIAQ
                     DREPQIVSVIGDTNVGKTVYLGFLLDMLTHRAGDFEAIPKGAYSIDLQQTVISNMSQR
                     MFPPKTPMEANQWYWAYYQVYKHSPQPKWVDLIMPDIAGESLAAEVAAPNTFKVIRSL
                     LNKSAGLMLLVDAALAANGSSQPDFFAVRMLSYIDTMYGAGRNQRIGTPTAILLCKAD
                     QCPDCFDDPRKFVKANMNRLWNLCESRLSNFEFFACSVVGSLGYATAPQEDYVIPVPL
                     HTALQGILEPFQWVVDQL"
     gene            complement(16786..17889)
                     /locus_tag="P5316_00995"
     CDS             complement(16786..17889)
                     /locus_tag="P5316_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR83558.1"
                     /translation="MGLALLSRRKNRQPQNNRGLPSHRRSDDYQPAEPLKLDQDPLQR
                     LLQMERYGAIVRNRDQWQDHAMIQSVFQAAVEAIDEQFALVPEGFASVSLAVNDFPGS
                     PEEDFETKPFLLARCCVTQEQYQKFVDSGGYEDLQLWPQDIWPHLIDFKDQTGKPGPR
                     YWQDGRHDKRFSRHPVVGICYYEAAAYALWAGYRLPTGAEWQMAASWRIRSSANVLRR
                     YPWGDSLDTAKCNIWASGIGGTLPVTEYENGAAPNGVLQLIGNVWEWNSSNYEITDDC
                     GRQVVGDMLLKEIRGGAYDTYFASQATSCFRTGLASLARMHNVGFRCVLDLDAKDQPL
                     SQTPPSPDLPTAGRTTPAQPAQGPSNDPERRPQ"
     gene            complement(17932..18987)
                     /locus_tag="P5316_01000"
     CDS             complement(17932..18987)
                     /locus_tag="P5316_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83559.1"
                     /translation="MLRSRAKEKEATEPVTETRSAIPNAVPSFDLSRIESTEQNLVSA
                     MQTVRELLEARAQVLESCRKALEDYAHRLTEEAKNHDQQAQELCEQYENREKTLAARE
                     AECQKREQELKEAQARLDALTRELSTQSEQVRSKSEAISRDEAALNSRKAALDQEEQQ
                     FKAEVAKIEARAAEIEKDLKQVESAKASLAEATSNFEKERSDWEKQRTSLGGLEEQLK
                     SQQAALVKEQEAIKAKQEEMNRLAASIEEKAKAVAEREATVASMQKELDGRMQEAAAA
                     RDSLAQLQTQLQQELEKVSQQKNDLLPQYGLSETELAQGKAPNRNVPSVAEQEARESI
                     ERFQKLCRDAKRKAIGA"
     gene            complement(18991..19689)
                     /locus_tag="P5316_01005"
     CDS             complement(18991..19689)
                     /locus_tag="P5316_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83560.1"
                     /translation="MDSRVKDIQEVRVPPPDEALRKAFADLDAGLASWKAVLGELRAW
                     ILEQAEIQAEVRRELQTKAEECARLQADLESRAASHSQPENQPQSGATAGPVEEQQSE
                     ARARTQVTTADQAKTSAPASEVPETALPANQPPHPPVDWSSDAVSKHIRVYVDPANPS
                     ANAPTETESPQQADEDEMLLASLDPETAKAIRVMRRMRGDNKSVRELLEQYQASRTNA
                     KADSSKKSWFRRGR"
     gene            20346..20783
                     /locus_tag="P5316_01010"
     CDS             20346..20783
                     /locus_tag="P5316_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83561.1"
                     /translation="MTEWEKEFAKKMELLHEQSTTCLEQFLQEDLQAAFDSISAFLAQ
                     WKFHTSTPQTQAGRRSFKFGLTEDAYVIVTFRLEGVDVLECETEYGLPAVGRVTGTRS
                     TSSLRKADQPWAESCFQKALDGLLVKLGELDARKKGREPVLVA"
     gene            21664..22791
                     /locus_tag="P5316_01015"
     CDS             21664..22791
                     /locus_tag="P5316_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83562.1"
                     /translation="MSRVRTRVVSAAMAAMLAVSPIAVISMSMVAGCGGGGCCLPTIP
                     CKCAFQFASGLLAIWLLRNNGLGADGISCWDLNANGTCDTATEDVNADGVCNALDCQG
                     LAGEPGQSIPGIDGLNCWDLNANGVADPDEDINGDGAWDAYDCRGTAGPPGSDGKDGK
                     NGSNGPPGPQGPTRFDLFIEDFFTYGRITDDVRGQGEVINIQEPILSAEYSSFGPIGF
                     RVSVPEIYTEGNPVTLRLFFWREAWSSVGCTTLRFDVFRLIPGGGIERYGANPVWLRL
                     EPGSMTNPGMLVVDLPLNTAPDGLGFPNDLYAPQMLAFEISVFGVGDTVYPPDLDGEY
                     TVLGAEIFESLSAADTGLSGVTVFTSSAGVGSFCGAGGGDL"
     gene            22852..24087
                     /locus_tag="P5316_01020"
     CDS             22852..24087
                     /locus_tag="P5316_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83563.1"
                     /translation="MKMMPRIKGKVAGLTMAALLAASPLTLISMSMVTGCGGGGCLPP
                     IPCKCAFQFAAGMLAIWLLRGGPDGVGAAGLHCWDLNADGTCDPATEDANTDGVCDVN
                     DCRGPDGPDGQPIPGVDGLQCWDLNGNGIGEAEEDANGDGLFNALDCRGPAGPPGNDG
                     ADGTNGQPGPPGPPGPDLFDWFIEDFFTYGEISNSVTGQGEVINIQEPILEWDDQIGV
                     NPIAFRTAIPEIYHDGNPVTLRVFLWREATEGRCTVLRLDIFRALPGLGIERYGVPLW
                     LRLNAAGLTNPDMLVFDLPLNVPGPKGVGFANDLFAAQMLAFELRVFGYGEGAALDAR
                     YTVLGAELFESASPADVMLKRVTLFTSEPDIDKVCNVCDPNSPWPGQCDDGNPETLDI
                     CQPLQEGGWGECYNIVVGP"
     gene            24193..25026
                     /locus_tag="P5316_01025"
     CDS             24193..25026
                     /locus_tag="P5316_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83564.1"
                     /translation="MRRIVLLTACVCLAGAVSSAHALMLYGVSYDEQIWKIDRATGAI
                     ELYKNTPGLRWFGATDGPSDESFFATGDGGSLFKIDIAADTVTAIGTYGGSVRLRSLA
                     YADPVSPATEGILYGSDYQNLYTIDLVTGVATLIGPIKPVGELFVGVWSMDYDPVAQA
                     LFITNLVNNPITHAITTKLYTVNPANAEATLVGEIAGALPIADIWYDRDSGKMFGVTQ
                     VNGQLYEIDTATAGATPIGTAPGENILGLAGAMIPEPGMLMLLGLSVLGVLRAGRRSV
                     R"
     gene            25529..27016
                     /locus_tag="P5316_01030"
     CDS             25529..27016
                     /locus_tag="P5316_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRR83565.1"
                     /translation="MAEWRVYPKLAQLKDAQELIARWAELGYDIPCDGRILTAAEGSP
                     LAEPMTVFGRRIGNRWCIQPMEGWDGTLDGRPSEWTRRRWERFGKSGAKLIWGGEAVA
                     VRHDGRANPNQLWINSNTQGDIAALREVLVGAHREVTGGSEDLLIGLQLTHSGRFCKP
                     NDNTRMEPRIAYHHPILDARFGIKPDDDSIVFSDDDLWRLMDDFVAASRLAAGAGFDF
                     VDLKCCHGYLGHELLGGHTRPGAFGGSFENRTRFIRELIGRVRAEVPGLHIGVRLSAF
                     DLVPFVDDPATRSGSRKGVGIPARFDHCLPYRYGFGINPDNPLEYDLSEPIRFLEMLA
                     GLDVTLVNITCGSPYYNPHIQRPAYFPPSDGYQPPEDPLVGVARHIDVVRQFKQRFPE
                     MILVGSGYTYLQEFLPLVGQAVVRKRWVDFVGIGRMVLSYPEMPIHSLRKGELDTRRI
                     CRTFSDCTTGPRKGLISGCYPLDEAYRHSQAGQKLKELKKAEEGG"
     gene            27030..27632
                     /locus_tag="P5316_01035"
     CDS             27030..27632
                     /locus_tag="P5316_01035"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRR83566.1"
                     /translation="MYKLSWPNRVTMTRILLAGPFVVALLHLQDPQWSELARWAAVVV
                     FVLMAISDGLDGYLARRLKAESALGRFLDPLADKLVVLSSVVLLARENTSVPGMQLPS
                     TVAVIAIGKDLIIVVGFCLIYMITSSIYIEAKRLGKWCTATQLFMVIAILLSPNVPGP
                     LKWGPVLLWWAASLLAIATVIQYFRMGLYFISRHESQKAA"
     gene            27693..29504
                     /locus_tag="P5316_01040"
     CDS             27693..29504
                     /locus_tag="P5316_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR83567.1"
                     /translation="MAAPTPKLRRRDILAAGAFAYGLLSTKRKLVAAPPEHVIVDVEQ
                     LREVSVDTTAEPKEQHSCDILVVGGGLGGVAAAITATDAGREVVLVEETAWLGGQAAS
                     QGVSALDENGWIETSGGTCSYLAFRKAVRDWYRRNAKLKPQAARKEHLNPGNGWVSRL
                     CFEPKAALAVIDDLLAPAAARNRLRVLKRCKAYAVRMEDDHVAAVDFVHLDSGDRFRV
                     RARYAIDSTELGDLLALGAAEFVTGFESRRETGEPNAPDESDPEDVQSFTYPFAIELR
                     RGERHLIPQPAEYERNAKHQPYSLDISRQTGRPPYAMFTTRPGTYGPFWTYRRLIDAS
                     QFDDPNYRNDVAMINWPGNDFRGGNILSGTPSQQIALLRAAKNLSLGLLWWLQHEVER
                     DEGGRGYPEFKLRPDVMGSTDGLSKFPYIRESRRLRAVKTIVEQEITTAGPRAVHFDD
                     SVGIGHYGIDIHETTRPRKLDFKPARPFQIPLGALIPRRTLNLLAGCKNVGTTHITNG
                     CYRLHPIEWAVGEAAGETASLCFERGRRPKDILANKELLRTLQRRLVARGAPIMWYDD
                     LPVGDPRFEKLQMLPFESPEVMAGIQGNLHAPDVSPG"
     gene            29578..33729
                     /locus_tag="P5316_01045"
     CDS             29578..33729
                     /locus_tag="P5316_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRR83568.1"
                     /translation="MWLPGRRMMTKLSARTWTSRLLVIWVLITAAGASGQPAASTSSP
                     TDAQQDSRFSELIRSADRYHESQACHLEAEALEKALQMLPPRHAQRRELARRLAAVCR
                     SGMGHYARAERWLQTLADELRPLPQGIDALFDLADAYFNHGRLNEALSVYREILKRIV
                     PPEPPYDVDDRCWKAWRGEDLCESPLGSDPLFPSDASVSGQVFRISNLAPKGSWEDIR
                     RLLDENLRLRLMIRPGQEAEVWTSLRAEARKAISRLPDDAMRRLVAAYERRLAELAGG
                     NDWRSAVEFRLSHPIPQLEGVLDAAIGDKLLDLGQPALASIYYGRAVQAAGQNAEPIL
                     LARELFSRIQSGETIPLDNVPEIRVGIGGQERTLRQWVEHWQPRISPAPLSKPVLDIG
                     RGVMTRVPMLRATCPLLQWQARWRARNWDDVYPYLDEFVPCIAAGSPRRLLINMGDAI
                     EAVDLTDGRIVWSFLPPEENPMPQPGQEFQARDAYVMCGKSKTAVVNGGRVFCHLLWG
                     HHDTPRDAGALFALRESDGAMIWSSLYVPQLAGIQFAADPAVYRGVVVAVAWRPRSVM
                     PMFFVVGMSAETGEVLWLNHLYSGGSMVAYDNEQFLDLPTACGPPVIADGVAYLTTGA
                     GVVAAVDVLDGTTLWATAYPRARAINREEWAVRMATSRPPGVVAVFRDVVLFAPVDAH
                     LLLAVERSTGRIRYMHRSVDLRAIAAADAERAYVVEGTTVRAVRPADGRTVWETQLVT
                     SGLVGLPTLADRGLLCPTWETLYLLDPANGGILAERDWDRAQACGYVRDFGDCMVGTS
                     QVGIHIIADRRLDDTGDRWLSPEYEHEPPELNVRSRAGNWLRWAMPGMVNRGDFVLSD
                     SDPSHFLCRSELLQMRDIDPVPTLQWERLSPLPWDGRVAFSPDWVAVWNIGEVYVLEA
                     ATGRSVWEDRPLVRLGRPISRVLILGKEVRVLLGAGKDRGTVPLYVSFDGRSGRDIVP
                     STGVDALWSERPDTGRRVIPDGPERLIEAHADGYRYEFRDNRLRAIDLWDTREQWGSP
                     TIIDDVRFIAPLSGGVLALTQARLEGHIRFRDMAHIQVFDRHDGERINAFRLFKRWQH
                     QFEHCDDRVMIWDINFLYCVQPPIAAAHGGEQVTIRQSRPDPDAIAALRLARDLETPP
                     QVSIGTLQRPPHVDAEFSEWSTVEPVELRDVMDWRPDFVHRSRWKSRFYGGGDDLSAK
                     VRLAWAGDSLCIAADVTDDAHVTTACPGLWRFDSITLQISEGGSEDVDPLMLTVGSVN
                     GIVRLEMSSPVMMLAASDPPGTERHCEPGIRRFALSRPGAAGAGGPEATLYVEAAVQR
                     DDFSRRTRYEIAMPRQLLRYGPESYWDLLINDNDGQGREGALQLASSIWSIEESQAAC
                     LRGGR"
     gene            complement(33753..35336)
                     /gene="serA"
                     /locus_tag="P5316_01050"
     CDS             complement(33753..35336)
                     /gene="serA"
                     /locus_tag="P5316_01050"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRR83569.1"
                     /translation="MYRVLITGKIHSNGVERLSKEPDLQVDFHPDMPIGEILQIIAPY
                     HCIIVRSETPVKRELIDAAKELKVIAVAAVGVTNVDVEYATRKGILVVNTPGKNTNSA
                     AELTIALLLAVTRKLTEAHATMQHLGWDRHRFQGVELMGKTIGIIGLGNVGHRVARFA
                     RGFEMRVLAYDPYIADEVFERHEAEKADLDRLIRESDIITVHTPKTRETTGMIGREQI
                     ARMKKGVVIINAARGGIVTESALLDALKSGHVAGAGIDTWEREPPDENPFRDLPNVVM
                     SPHIGASTTEAQIRVAESIAVQVPRALRGGIVDYPVNMPQIRMLEGGDIMTSYTVLAE
                     RLGTFAAQFMDWVPERLQITYRGSIARHDCTLLRLAFLKGFLKNSLDYVSYVNAEDRA
                     RSIGLKVEAICDPGFTDYESEVRYGFKGQDREFNIGGVVFSGPHPRISLVDGFEFEVE
                     PEGTFLAIRSKDRLGVVSGISSLLDKHKIQIDSFEFSHSKERKRSMFLIRVRRNVGDD
                     VVEEIRRQEHITMARKIQI"
     gene            complement(35684..37891)
                     /locus_tag="P5316_01055"
     CDS             complement(35684..37891)
                     /locus_tag="P5316_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRR83570.1"
                     /translation="MTSLSPKLTDLANRNEIQALENLWMDMLESGSEDREEMLEALAV
                     LTRNGKGQQAGALAWMWLETKQERSEPRELLDLVREMIVRCDDSDDLREAAVELYQRV
                     YADRPEIGNLIDMSGLSGGKSVRRALRTLDICLNVKVGDFLVSRSDEHAAKVTAADAK
                     AWLYTIRTDHGEQTMDADALALAYDPAEPNDFRVLLQMDPDGIRNMIDNDPTSLVLGI
                     LRSHRGRIDSDELAHLLSPRFIPERDWKKWWSKAKTDLKKCPNIVVEGKARTILTYHA
                     KGQTLEDEVLPQWTAAETPHQRMAVVDAYFREARNRQVDPDAGIIARFQADLQTRIDL
                     VRERAPAEALAEALVLDRLAEQASLAERDAGPAKAILSGALDPVALLGTIEEHPLYLR
                     AVALVRQARPQDWPEIFARLLPPAHREACDVIAQSLIEAGRQDLLEQAVRQIPSDFTR
                     HLDAVCWLWRGPAVEIPEAMPLREILLRLLDHLGNLTRSETTSAEVLRHARSEIRAAL
                     SAAKYARFRKVISEMEPKLASTVHRTIDRLDGLGEVVHADLLKIIVHTHPELISPKKR
                     VDPWTDENTIYCTQAGMKKREEELNHLRLVKIPENAKAIGEAAARGDLSENSEYKFAL
                     EERDLLQARVMRIQNELAIGRLLTVNDISTEEVGIGTRVTLQAVDGNDRREMSILGPW
                     EADVERGIFNYQAPVCMKLRGLRVGDTVELDLGNGPGNYRIEAITNALEGQAP"
     gene            complement(38149..38811)
                     /locus_tag="P5316_01060"
     CDS             complement(38149..38811)
                     /locus_tag="P5316_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRR83571.1"
                     /translation="MKQVCVIGLGQFGSHLARTLARMGCDVLAADMSERQVAAIRDDV
                     QQAVVLDSRSFEALESVVNKDTDEAVVSLGEHLEASILATLHLKQIGVKRIHAKASSK
                     DHAVILKAVGATDVIFPEQETAERMAHRIVNPDLLDYLPLSREYRVVEIGVPPRFVGQ
                     TLAQLHVRRQYNVLVIAIRKSKGTEPEFMPPADKTLQSGNTLVMIGKEADIEAFSKAG
                     ES"
     gene            complement(38822..40192)
                     /locus_tag="P5316_01065"
     CDS             complement(38822..40192)
                     /locus_tag="P5316_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRR83572.1"
                     /translation="MLRPPGLILFSRLLDSPERFLAGTFAGLILCGTILLSLPVSHGK
                     TQVSPLDALFTSTSAVCVTGLVVVDTGQDYSRFGQVVIMLLIQFGGLGIMSFAALIMQ
                     IAGQKISFRSQAALHDAFYQKSAAVHLRQNLAWILSTTFLTEAAGALVIFCTRPAGMS
                     SGEGAFMAVFHAISAFCNAGFSTFSGNMQDVAGNMGILSTIALLIICGGLGHTVILEL
                     AGRAWKRLRNKPRPMVWSLNTRVVLATSLVLIFGGAIVLHLLGLAASEPGLLAQAGHC
                     LFQSISARTAGFNTVDLNQSPVPALLWLVFLMFVGGSPGSCAGGVKTTSLAIWAARLR
                     ARLRSREDVTIGGRRIPVDLVRRTGLLIATASVFNLVGLMVLAITEMRDPNIRLEQIL
                     FEQISAFGTVGLSTGITPSLTIVGKLWIILTMFVGRLGPLTAALVVMEHKPEAVRLAE
                     ERLMIG"
     gene            complement(40304..42070)
                     /locus_tag="P5316_01070"
     CDS             complement(40304..42070)
                     /locus_tag="P5316_01070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR83573.1"
                     /translation="MTRHPYFICVLAALTLGQMCTQQDGPDKPEPLTLPRVKLVTTRG
                     SLTIELFTNHTSAATTFKELVDAGYYNNTIFHDVRQGKWIVGGQYYANMDRNVKKPLV
                     NDSSNGLRNTRGRVSLYGPTREFSEGVPQFLINLADNTELDYTPGSGEPVDYTVIGRV
                     VDTGGMSVADAIGNAPTGTRNTNDDPPELLSHVPQEDIIINDAYVGAGVDADAGPDLV
                     GLLNQDVVLDGSGSESLYDKKLTYAWAQTGGASVTLTSVNTGQAKFRPGATGTYTFEL
                     TVTDEDGESDSDTVKVTIVSEPNVRLVTTKGDIVLEMYEEGAPITVANFLTYVQSGFY
                     NGTIFHRVIAGFVDQGGGYLPDLTQPSGLRAPIVNEFDPSRPNRRGTISMARTSDLNS
                     ATSQFFINLVDNTNLDNPRSGYAVFGRVVEGMNVADDIARGATETREDPNGNPFDDVP
                     VEDVVITSATIEARPPAPQFVTTASGLQYKDIAIGTGPAVTADSTIKVYYTGRLTDEN
                     GEIFDSSGETKTNPATFTLTDLILGWQEGLTNYDMRVGGTRVLIIPPELGYGAAGSPP
                     DIPPNATLCFEIEVVEVQETSE"
     gene            42204..42770
                     /gene="folK"
                     /locus_tag="P5316_01075"
     CDS             42204..42770
                     /gene="folK"
                     /locus_tag="P5316_01075"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRR83574.1"
                     /translation="MPISGPTVQQATGDAIEAASVSHVAYIGLGANLRDRRANILAAT
                     DLLRQAPRVASVSLSSLYETEPAGGPAGQGMYLNAAARVETDLDASALLAVLLDIEAR
                     LGRQRSEKWGPRTIDLDLLLFDDQVIDTPALTIPHPRMHERRFVLEPLAEIGGNAVHP
                     VLRLTIQQLLDRLPEQVLKRDGPASPLR"
     gene            complement(43098..44153)
                     /gene="ruvB"
                     /locus_tag="P5316_01080"
     CDS             complement(43098..44153)
                     /gene="ruvB"
                     /locus_tag="P5316_01080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRR83575.1"
                     /translation="MARERVISTTSMSMDEERFNESLRPERLDEIVGQQKIVEQLRIA
                     LEAATKRQEPLEHVLLAGPPGLGKTTFAQVIARELGATIRITSGPAINKAGDLMHWLT
                     EMKRGDVLFIDEIHRLNRSVEEFLYAAMEDFRVDFTIDSGIGGRTVNIALRPFTLIGA
                     TTRSGLLTAPMRDRFGIQFHFEFYTREDLAEVLRRSARKLKIEAEEEALARIAARARG
                     TPRVANRLLKRTRDYAQVRADGKLTAAVVEKALSLLQVDHLGLDELDRSFLNSLIKTY
                     GGGPAGIEALAATLGQERDTLEDVVEPYLLQIGFVIRTRQGRSATKAAYDHLGLPFTP
                     SAAAAEEIHETLFDEAE"
     gene            complement(44160..44795)
                     /locus_tag="P5316_01085"
     CDS             complement(44160..44795)
                     /locus_tag="P5316_01085"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRR83576.1"
                     /translation="MIVRMTGTISEVHEGYMVIERDGVGYEVLICGYAAAELAACQGQ
                     ERTLHMMEYLEGSAAGGNLIPRLVGFLHPEDRAFFERFITVKGIGVRKALKALSQPVA
                     AVASAIESSDARSLARLPGIGKRAADQIIAELKGKVTEFAAAATELESAAISAESWPP
                     EQRDAMEVMIALGERRADAQRWLARAAQLHPGEHPPDEWIHLAYRIRSSEG"
     gene            complement(44804..45346)
                     /locus_tag="P5316_01090"
     CDS             complement(44804..45346)
                     /locus_tag="P5316_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRR83577.1"
                     /translation="MSATTQIICGVDPGLRQTGYGVILVDDEEDTVRVLDAGVVRCSR
                     SEALSLRLAELATGFAEVLDDHRVELVAVEEIYSHYSRPRTAILMAHARGVILLEAAQ
                     RDIRVIHLPATSVKRHVTGNGRATKDQMQRAVTDLLKLHRIPEPPDVADALAIALCAA
                     CVIRQPARVPPSNNDQLLSH"
     gene            complement(45569..47080)
                     /gene="cysS"
                     /locus_tag="P5316_01095"
     CDS             complement(45569..47080)
                     /gene="cysS"
                     /locus_tag="P5316_01095"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRR83578.1"
                     /translation="MPLRVFNTLTQSKEPFEPVQPGKVGMYVCGPTVYKPSHIGHAVG
                     PIIFDTVKRYLQFKGYEVTWVVNITDVDDKLIDEAKAQGCGVMELAERVTASYLDALR
                     QLNVTGIDHMPKASETIADIIAMCRRLIDKGAAYVSGGDVYFDVSKDADYGKLTRRRP
                     EDQQAGGRELASGEKRNPGDFALWKATKPGEPPEVQFDSPWGKGRPGWHIECSAMSAK
                     ILGESFDIHGGGMDLMFPHHENEIAQSETCFDKPFVRYWMHNGLTRFNTKKISKSDPE
                     MRRLMDQLTLTNLLSKHSGEMLRFFVLSTHYRRPIEFSDEELAAKKKGLDTFYRLFDR
                     IQRITGSDPYAGGPGLEATSPETKSASARAFVKQCQEHRDRFIEAMDDDFNTGAATGV
                     MFDLATAVNRFIDTEKLDSGGDPETKQMAMQAVQTLRALGKILGLFEKRPAVPVSAGD
                     TMVDELMQILITVRAEARKAKQFALADIIRDRLAALSIKLEDRPDGTTWRKGA"
     gene            complement(47319..47819)
                     /gene="ispF"
                     /locus_tag="P5316_01100"
     CDS             complement(47319..47819)
                     /gene="ispF"
                     /locus_tag="P5316_01100"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRR83579.1"
                     /translation="MNDQAATGIDRVGIGTDLHRLKAGRRLVLGHVPIEFDQGPVGHS
                     DGDVLLHAVIDALLGAAGLPDIGELFPDTDAAYRGIDSARLLERALALVADRGFVPVN
                     VDAIIHAERPKLGPHKKAIREEVARLLGLVPEAVSIKAKTNEGVDAVGRGEAIACTVV
                     VGLRRT"
     gene            complement(48083..>48305)
                     /locus_tag="P5316_01105"
     CDS             complement(48083..>48305)
                     /locus_tag="P5316_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83580.1"
                     /translation="CVKSRKPCYAPAETGHRTISIAHIGNIAMLLGRKLKWDPQAEEF
                     IGDAEANAMLSRKQREPWTLANIDSWLKA"
BASE COUNT         8630 a        14683 c        15328 g         9664 t
ORIGIN      
        1 gggtccccct cgtccgcatt gcagatcaga accttccggg ttccaggcgc ctgagcagat
       61 accttcgagc ttctcgaagt agtcccgggc cgaagtgacg tcgtggctga agcctcgccc
      121 cgcattccag ccgccgccga ccagcatcac atgcttgccg agggctgcgg cctcggcagc
      181 caccgtcgaa gtgtatacca aagcccagtc cgacaatcgg gccacggcgt gagcgctgac
      241 gtccgactcg ggtggcaaca cgtggatgtt cggcgggagg cttggaaact cccgttcaat
      301 ctgtggaagc agcgggtcgc gggctttcca cagcttctcc gaagggtgga tcttgaccac
      361 caggtgatgc tcaggccggc ggcagaagaa gcggatcgtc tcagtgatcc aatcgttggc
      421 tgactcgaat gcggcgaccg tcttgttccg gtcggcatcg tagctgaggt tcgggaacat
      481 cccgaacacc ttgcccgcaa tgcggtcggg caggccggca tgtcggcggg cttcgcgaag
      541 atcggtggtg ttggtctgcc agtcggcgct gcggtactcg tagggcacgc gttcacggcg
      601 gagcatatac tggtccagcg ccttttcctg ttccggtgtc aacgggatgt gcttccatgt
      661 atcccactcc ggcatcatcg ctgagcggtt cggcggtcga tcatgttcaa agcgaatgct
      721 gtaatagccc aggcctgacc gtatgacgtc caccggaatg cccatcctgc cggccactcc
      781 gcgaattacc ccccaatcaa ctttctttcc gtcctcttcg ataaccttgt cgaccttcaa
      841 ctgttcgagg gctcggaagc cgacggtggt catgtacaga gccgcccgga cgaactgccg
      901 ggccatgccg gcggtctcgg gatcattcgc gtcaaagtag ccgatcttgt agaagctttc
      961 gattgattgc cggacgtgct cgccgagagg aacgccccga tgctcaaacg catgcagcgc
     1021 gtgcaggtcg acgtcggcag cgatccggtc gcattccttg cgctcggcag gcgtgatgaa
     1081 ctcgctgtac cagcggcagg gcaggccgat agccctggcg aagggccctg agttcttgtg
     1141 gcaccagcgg gtgcaggcgc tctcgtagtg gtcgactttt cgcatcgtgc aggccggcaa
     1201 gaactcatcg cactgcagca aggtgaccgt tgccccccgc atccgcaacc cttggccaat
     1261 aatgcccatc cgggagaagt gaacctcgcc cccgaccact ccggcgatca agacgttgcg
     1321 gtacttgagg tccggccagg gatcgaccgg cagcggatcg caggcaatct gccgaagctt
     1381 ggcgatgaaa cccgggcctt tcagtatcgg aacgcctacc ttggtcttca tatttgccct
     1441 gcaggccctt tttcgcagtc accttgccag gtccgctgcg gctggctcgt cgggacctgt
     1501 tggatgtaac atcttgttcg acaatgagat acggccgcat ttgggcagca aggcggtctc
     1561 attgccctgg atggtttatc ggtcgtattc cggccgtggt taagcaaagg tctgtgcgcg
     1621 gctgccccgc aggtgggtcg aacagggcat cggtttcaag ttctgaagcg cacgacccgg
     1681 gcggggttgc ccatgaccac ggcccaggcg gggatgtggc ctcggatctg ggtgttggcg
     1741 ccgatgacgg cgccgttacc gatccgcgcg cctcggccga caaaggtata ggccgcaccc
     1801 cagacgtcct cgccgatgta gacgggttcg tgccggtagc cctgccgcat gaagggctcg
     1861 cgggtaaggt agttgtgttc cgaggatatg atggtgcagt aactggcaaa ggcggttcgc
     1921 cggccgatga tcacgtcggc accagcgttg acgatcgtgc ccatgccgag ctgggcattc
     1981 tcgccaagaa tgatacggcc gtcgcgggcg gcaaccagcc tgacgttggt gtcgaggacg
     2041 accccgtcgt gcaagatgat gctgccgttc tctcggatct tgaggtcaac gccgggcatc
     2101 agcgttacat cctcaccgat ggtgatgttt ctcggatcgc cgtcaaatcg gagcatcaga
     2161 ggcccgagga cgcggaggcc cttgccgacg cgtatgccat gacggcggaa gacccagcga
     2221 tagtagtgtg cccagaagcc cttggttcgc cgggtggtgc gaggggcccc gatgagcaat
     2281 ggcacgtcgt caggaccgtg tgggaaagag tcgaaatcct ttggcccggg agcccccgtc
     2341 cgcaggcgat cgaccaggtc tgcgattctt cgaatcgtat cgatgtttag aacgtcggcc
     2401 agtattggcg tcggtatctg caccctgaag ccggtttcga cggccgtaag cagcgagacc
     2461 agtccaagcg aatcgatcaa cccggaggtg accagcggct cgtcgtcacc gacccgggcg
     2521 aaccgaccgg cccgaacctg cgacaggacc tgggtcacgc aacgtcggac cgccgagagc
     2581 gtcgattcgg cggtcgtggc ggcattgggg gagcacgatg cggattccgg cgatcgtgaa
     2641 tcggcgatca tctcaatgta tcggtcgcga ttgacgtttc gcgagatctt tcccgaggtg
     2701 ctcttgcgca gccacatatg ttcgagcagg cagacgtcgg caggggcgac ctcggttgcc
     2761 cgggcgatac gggcgagcac gtcgcgtcgg atcgattccc gtttctccgg atcggtctcc
     2821 tgcgtctcag caaggatgat cagatcttcg gttccggacc gctcattggg cacgccgatg
     2881 gccgcgcaac ggccggggat gatgcccggc gtcgcgctga cgatttcctc aatatcctgc
     2941 ggatagatgt tctgcccgcg gatgatcagc atgtcgttct tgcggccggt aacatacaac
     3001 tgcccgtccg ccacatagcc caggtcgccg gtgaagtagg caccgtcgcg gagggcttta
     3061 gcggtcgcct cagggttgtt gtcgtaacgg gtcagcagac agggcgtgcg gagaacgatt
     3121 tcgccaagcc ggcgatcggg cagcggccgt ccctggtcat cgacgatagt aatctcggtc
     3181 tgcggcaggg gggtgcccga gctgatcatc ctgcggcagg ttggcgaatc ggcaggggca
     3241 ggtatcgcct gaccggttcc ggcgaacgtc ggtccatcga tgcagtcgtc ggccagcggg
     3301 ccgtcaaagc cgcccgaagt gacggcaaac gtgttctcgg ccatggcata cgagctgcac
     3361 aaagcggtat tgctcaggcc gcaggccgcg aatcgccgca caaactgctc atggctacga
     3421 ctcagaaggg gttcggagca gttcacgatg ccgcgaaggc tcgaaaggtc aatgcccgcc
     3481 agctcggtat ccgtcacggt cgttgccatg aagctgtagg cgaagttggg cagccacgac
     3541 agcgtgccgc ggaagtcgga aatcgcttgg agccacatgg ccggacgctg gacccactcg
     3601 aaggggcaca tggcgaccag aggcacttgc ctgataaagg gcagcatgaa gcaggcgatc
     3661 agacccatgt cgtgatacag cggcagccaa ctgacgatgc gatcggacga atcgaggcgt
     3721 atggcatccg cgtaatggtc aacttgccag agcagtgccc ggtgcgatat ggccacgcct
     3781 ttcttggcgc cggtggtgcc gctcgagtac tggaggaagg cgatcgtctc aggatcgtcg
     3841 ggcatgtgcc acgcgggtgc ctcggtagtc ttgggaacct cgtcgacgac atacgacggc
     3901 acggccgcat ctcggaacat cgtaagagct tccggctgat cgccgagacg ggagtaggtc
     3961 agcagcagtc tgggtttcgc tcccgccagc agcgaggcga gcatcttgag atactcatgc
     4021 gctgagatct tgggtgatgg acaagaccag aagctgggca cgaacccgcc cagaatcgcg
     4081 ccgatgaagg atgcataggc gtcgagcgag tgccgaagga taatgaccat tcgatcgccg
     4141 gggaccagac cggcctggcg atagaaactt gtgtagcgcc cggcttcatt gagcacgtcg
     4201 cgatagctga accgttcggg ttcgccgtcc gcgtgcagca ggcagaggaa ctccgcgcag
     4261 tcgggcgttt cggcgttctt tgccagccgt tggggaatgg tcgcgggcat cgtctcaatg
     4321 acgctcatca agccactcac acaattcctg ctgatggtcg cgggcataca tcaggaaccg
     4381 ttccatacgg tccttctcga ccagcatgat gtagtatcgc ccctgttgat tcctcaccag
     4441 ggcggtcttg aagccctggt cgcgaagccg ggcgtttacc gaaggcgtcg catagaagcc
     4501 ccagtccttg gcagtgacgt catactcgtg gccgcgcggg gtgacgaagg ttaccatttc
     4561 atcgggatcg aggaagactc gcccgcagtc ggaaatctcg atcgccccgg tcctgccgac
     4621 actgaacttg cggggcggat tgttctcctc aaacttcatg tccacttcca ctccttgatc
     4681 aggaaatcct tgatgaccca gcggccgtct tccctcgtcc agatggcggg gttgcggaaa
     4741 cggtcctcga tctgccggaa ttcatcgacg gtgataccca gatacttgca gaagccttga
     4801 atatcgtcat gcggcgtctg gtcgccgcgc tcggcgatga ttcggacggc ttcctcgcgc
     4861 gtcaggcgac cgttgcggat ctcgatggcc aggttgtccc acagtcgggt gaaaccgaac
     4921 ttgaaccact tgaaatggtg atgaacggag atgaaatggc aatcgatatc ggcatagtcg
     4981 tagaggccga cgcggggtcc tcgagcgtcg gcctggaatc cacgcgcggt cgcgacccgc
     5041 ttgctttccc atgggtccca gggcaggtag tagccgagga agatcgacat gatccgcttt
     5101 cccgagaact gctcctgcgg cggcaggcgg tacggttcga ggtccttgag agtgagttct
     5161 tcgtcgaccc agtcctcggc cgtggtgcct tgcaggattc catggcgtcg aagggtgtcc
     5221 gggtcccagc ggtcgatctc aatttcccgg tcggttccgc cgtactccat gtaagggctt
     5281 tcgccccaga cgaccatcgg gatatccatc gctagtgcga tcttgagcgg tatggcatac
     5341 agggccatgt gcatgggcac cgccgagctg cccgtcttgc gcagggcctt gagcgtgaac
     5401 cgccgctcca cttcgggatg aatcgtgaaa tcgatatgat cgacgcccag gcggatgaga
     5461 ttatccaggt tgcgttgacc gatctcggtt cgtccgggcg ttcgccacgt gaccgcaagg
     5521 atgcggaggc catattcgag gcagacgatt gtctgccacg tgctgtcctt gccgccgctg
     5581 acggggatga cgcagtcgta gccgaggctt cgccgcttgg cctccgagat gatctctgcc
     5641 aaccgttgct ttctgcccgc ccagtcgatc tctcgcttgg cggcgactga tttgcaggca
     5701 ttacaaaccc cctccgagtc gagaacgatt ccagggcggg tgtcgggcag gacacagcgg
     5761 gtgcagtact tcatgattgt gtgctcccgc agagtgccgt atcgcggtcg ggcgtcacgg
     5821 aacccggagt gccgcgggcc cattgctcca ggaacagctt ggcgctcaca aagttgaaca
     5881 ggaacttgct gaagctgttg ctgagcgtct cttcgccgag cagcgcctcg atccggtcgc
     5941 ggcggatcag atcgaagatc ggcccctccg agagcaggaa atctcgatcc tccgggtcgc
     6001 gcgggtcgaa aagagcgtgg aaactcgcgt tgaagccttt cttctgccgg tcgaggcgaa
     6061 ccttgtcgtt gagaatgccc ttgaccgcct cgcgcagcac gtatttgccg tagccgtcgc
     6121 ggatgaggtg ctcgggcgga atcgaaaagg cgaactgaaa caaccgcgag tccaggtaag
     6181 ggctgcggtt ctcaacgctg taatacatcg agttcaggtc atcttcgtgg aggatgaccg
     6241 gcgttgcctc gtggaacaac tcattgagca tccggtttcg caacagggaa tcgcagaatc
     6301 gctcctcgaa gaaggcctcc ctgaagtcgc aggtgagcat ttccgcgaat acgtggttat
     6361 tgcggtagat gtggtcgcgg aaatccgggt tgtcgaagta aagtcctggg ttcttgaggt
     6421 gcgggttgcg aacaaatcgt cccgggccgt catgccagtc ggcaagccag gtggcgaagt
     6481 ccggatggtg ccgaacctcg tacaggtgca ggttgaaatg gtcgtaatag ccggtgacca
     6541 gctcgtcggc ggccgtaccc gaaacggcca cccgaaagcc cccgcgagag atcatctccg
     6601 aaaggcacga gtggatgcaa taggtcaacg tgtagaccgg ggcgtcatgg tagcgcacca
     6661 attcctcgag acgccgccgc atgtcccggt gcggaatgct catgacgtgg tggcggcaac
     6721 cgagatcgtc cacggtggcc cggatattgt cgaactcgtt gtaccgctcg tcctcgtcgc
     6781 tgatggagaa agtggtgacg tcgtagttga agaccttggc ggcgatggag gcgatggccc
     6841 ccgagtcgat gcctccgctc aggcaaaagg ccagtggcac atctgccctc aggcgtatgc
     6901 ggatgctctc ctgcagatag tgacgaaagc cctcgacggc ctcgcccagc gtcatgtcgc
     6961 gaggcgtgta ctgcggcacc caataacgtt gcaggcgcgg cctgagatcg tcccggatct
     7021 ccaggttcga cgcatacggc acctcgcgca cctctgtgaa aaacgtctcc ggttgcttgt
     7081 agagcgattt gtagccgttg acaaggtaac ggaggacctg ccgctgattg atcgccagcg
     7141 gtctgtcggc gagggcttga agaaacttga cctcggaccc gaaatagacg ccgtcatcgt
     7201 cggcgatgta atacagcggc ttctcggcaa agcgatctcg cgaaagaaag atgcggtgct
     7261 cgcctcgatc ataaatcgcg aagctccaca tgccctcgaa cttctcgacg caacccggtc
     7321 cataggccag gtatgattga agcaggactt cggtgtccga ctcggtgttg aagctgatcc
     7381 cccgtcgctc gagatcctct cgcagctcaa cgtagttgta gatttcgccg ttgaagacca
     7441 ggtggtggtc gccgatgctg aacggctggt tggagcgagg atccagatcg aggatgctga
     7501 gcctggagtg gagaagcacg acgtgaaggt cgccatcgcg gaagaccgta tgggcctgat
     7561 ggtccgggcc gcgattgcgc atcagggcca gcgtccggtc gattctatca ggaccgattt
     7621 ccgaccggcc gatgtatccg gcgatgccgc acatacgagc gcttccgtgc agggcggacc
     7681 gcccaggccg cgggccgcaa atgggggtcc ttttgagcag ggccacgtgc gcaaaatgcg
     7741 cacttgtccc gtcttaagag ttcaccgtct tcctggccga cgtcggcgca gggattttgc
     7801 ccctatgtcc atcggaaccg ttggcggtct gtctccaata cggctcggaa agtccccccg
     7861 aacccggttt ccgcggcctg cggcgccagc cgaaggcgag gtttgtctgg cattcgatgt
     7921 cccgggttgc tatactgtaa gtatcgtggc ggccaaaggt tgcgaagagg cgttgccggg
     7981 gacgcgggga atcgggagca ccaggcttga ctaactcgaa ggacattgcc gccgctgttc
     8041 tactgagcct tggatgcggg gccggtgccg ttcttgccgc accagtcgac aggccgcggg
     8101 cggccgtgac cgcccaaggc tggtggtcgc ttcgcgccca gacgacgctg aagggaaggg
     8161 gattgccggc ggatgggccg ggggccctca aatcgcttcg atcggcactt tcctcaaaga
     8221 ccgcttttgc cgtggagcaa ggtcgatctc ttctggacga gcagggtgtc gttcgcgcct
     8281 accagtttga cctgccaggc ggcggggccg tcgttattgc ggccgacgat gccgtttccc
     8341 ccgttttctt ctactccttg gacggcaagt tcgaccccgc tgtgccgccg gcggcggcga
     8401 tatgggatga gttttgtcgg caggtgaagg agaacgcgtc tcggcccgac ggcaacgccc
     8461 cccatacttc ctggcgagta atcgagcagt catttgccgg tcggcttgat ccggccttca
     8521 cagcggcggc gaatcctatt cccgacgtcg gctcatcggc gatatcccgc gggccgctcc
     8581 tgcggacgcg ctggcaccag gatgagccat acaacctctt ggcacccgag cggctcgact
     8641 gtactcttcc ggcccattac tgccgatgcc cgatcggttg cgtggcgacc tctcttgctc
     8701 agatccttgc tttttggcag agcccgcttt tcggcaccgg gggtccggcc ggcggagagt
     8761 gctacacctg gaacaacgga gatcctgaat cctcgcgttg gccctcgctg tgtttcgacc
     8821 gctcgtggta tgacggccgg tatctgccgg tctgtgacga tgccgacgcg cccttctacg
     8881 actggcacaa catgtcggaa acggtttccg ccacggaccc gtctgtgatg caggatgcgg
     8941 tcgccaagct gtgttatcac tgtgcggtgt cgctaaaaac gggtttctgc agcggcgggg
     9001 agcagctatc gggggcgatt agcgatccgg ccgtgcctgt tcgctatttc ggttttgcgg
     9061 ccgggggctg cctcatctcg aaatcaggtt tctccgaaga ccattggttt gaggagatca
     9121 agcggcagat cgatttgggt tggcctctgt ggtatgacgt tcccaaccat agcatcgtgg
     9181 tggacggtta tgccgaggat atcgtcggag gcacgagcga acgccgtttc catgtgaaca
     9241 tgggatgggg cggcgcctca aacgggtggt atctcatcgg cagcatgccc aggatgcccg
     9301 gttacgaggg ggcagtggtg aaccttcgtc cggccggttt cggcgggggc ccgcgaatca
     9361 gagaagtgga cgccgacggt cgatcgggtg aatacgccac tattcaggcg gccatcaacg
     9421 cttccaccga cggtgacgag atcgttctga ggccgggggt ttacaccgga tggggtaatc
     9481 gggacctgga tttctggggc aaagcgatca ccgtccgaag cgtaaacccg gacgatcccg
     9541 ccgtcgtggc cgcgacgatt atcgactgtc agggcgtgca atcgcgaccg cgacgcgcct
     9601 ttctgtttca cagtggcgag acgaccggct ccaccgtggc cggtttgacc attacgggcg
     9661 ggtacgcgcc gacgcatttg attggcgatc agatcgtctc tgtgggcggt gcagttcttt
     9721 gcgccggagc gagtcccacc atacggcaat gcgtgatcag ggccaattcc gccgggctgg
     9781 ccggaggcgg ggtcttcagc ttcggcgagg ccggccccgt catccagcgt tgcgtcatcg
     9841 cgggcaactc ggctgccggc ggtggggcag ttgcagcctg gcttgacagc agcccgaagt
     9901 tggagagttg cctcctgacc ggcaacaccg cttcagaagg agcagcgctg cactactggg
     9961 aggccggccg ggcggaggtt gtcaactgca cgctcagcgg caacaggttg accgacgacg
    10021 acggccggat cgtcgtttgt cagggcggcg acgtcgcgct gaccaactca cttctatgga
    10081 atgaaacgac cggcggagcc cgcgagattg ccgtgcgaaa cgcgcagtcg tccagccggg
    10141 ttttggtgtc acacaccgac gtccgaggtg ggcaagcggc tgttgtggtg gagtcgggag
    10201 ccgttctcga ttgggcggcg ggcaacatcg aagggaatcc gggtttcatc tccgcgtccg
    10261 gagccgatgg ggatcccctg acgtggcacg acaatgatta tcggcttctt gatggctctc
    10321 cctgcgtgga ggcgggtgac aacctggtgc tcgcgggtga aaccgcgaag gatctcgacg
    10381 gcaacccccg ccgggccggc tccaatgtgg acatgggagc ttacgagttc ggctcgttcg
    10441 ccgattgcga cgccgatggc attcccgacc gtgaccaaca ggaccgcgat cacgacacac
    10501 ggatcgatgc gtgtgacaac tgtcccgaca gcccgaatgc cgcacaagaa gacttggaca
    10561 gcgacggtgt tggcgatgcc tgtgaccccg atattgatga tgacggggtg agcaacggga
    10621 acgataactg cccgcggacg ggcaatgccg atcaacagga caccgacacc gacggcgttg
    10681 gagatgcgtg tgacaattgc ccgctggtga gcaatcccga tcaggctgac gcggacggag
    10741 acggcacggg cgacgagtgt gcggccgcca gactctatgt ggatgcgcgc gcctcgacgc
    10801 acggcgacgg gaggagttgg tcgacagcct tcgccagttt ggagaaggct ttgtcgtcgg
    10861 cggccgcgtc cgcaggccgc acgacggaaa tctgggtggc cgcgggcgtt tatcgaccgt
    10921 ctcgaaggat cgttcctgag atagacggtt ctgccacctt tgtgattccg ccccgcgtcg
    10981 gcgtttacgg cggcttcgcc ggctcggaga ccgcccgcga ggaacgaagg ccgctgctga
    11041 accgcaccgt gctgagcggt gacctgaagg caaacgatgt tcccggtagc ggacgcagcg
    11101 gtccgagtcg acgcgacaac tgtcatcacg tggtgacgat cgaggggggg gacgcgacaa
    11161 ccgttctgga cggcttcgta gtcacggcag gaaacagcat gacccgaggt ggcggcatgg
    11221 tcatcctcga cggggccccg atcattgccg attgccggtt cattgacaac cagagcagcg
    11281 atggcggagg catctacatc atcggggccg ctccccggct gaccaattgt ctctttttgg
    11341 ggaatcgcgc cgtgaacggc ggcggtgttt acatcggcag cggtgatccg attctgtccg
    11401 gttgcgtctt cagcggcaat tcgtcttcct tcggcgcggg gggccttttc tgcgggctag
    11461 gcaggacgac gctgctcaac tgcaccgtga tcggcaacag cacaagcgcg tacggcggcg
    11521 gggcgtacat tgcttccggc gcagccgcca acgtcacgaa ctgcatcttc tggggcaaca
    11581 tgcggcaagg ggcggcaagt gcgaagtcgc agcttgaggt ggccggcacc gcaaccgtcg
    11641 cgcactgcgc gatccagggt agccaggggc tttgggaaga ccagaacaac acctggcgca
    11701 atccgatgct tgtagatccg gacggtcccg atgacgtgcc gggtaccgag gacgacgatt
    11761 ggcatctcct ggcctcatcg ccttgcgtgg acgcaggtac accgcttgcg cccgatgcgg
    11821 tcacgccgct cgatgtggac ggcgaggcca gagtgcggaa ttgtcgcatc gacatcggcg
    11881 ctgacgaatc ggctttccat cgcgactgca acggcaatgc gatacccgac gcctgtgagg
    11941 tccttgatgg aaccggctcc gattgcgacg caaacggagt gttggattcg tgcgaggtct
    12001 tctcacgctc gcgcctgctg gtgaccagtg cttgggggga taaggtgctc agcttcgacg
    12061 gcacaaccgg cgactacttg gggccgcttg ttgatcggcg ggcggccgat ctccgcgagc
    12121 cgcgggcgat tgccattgac agcgatcgaa gagtgtatgt ggccgccgcg ggcagcgaca
    12181 gggttgtcga atacgcgggc aacaatggtc tgcccgtcag aagcttcgcc ggtcccgact
    12241 tgcgtcgccc gacggcgttg ttgttggttg agccgactct catgctggtt gccaatggtc
    12301 aggacaacag cgtggtgcag ttcgacctcg atagcggccg gccgttgggg atcctcgttc
    12361 agtctggtga gggggggctg gcggggccgt cggccatgct tcggtcgcct gagggacatc
    12421 tgctggtggc cagtcagcag accaatcggg tgttggaata cgattggaag accggtgcgt
    12481 tccgccgggt cgtctgcgaa ggcgctggtc tgtccgggcc gagttcactg ttgctcgaaa
    12541 agggccgcaa tctcctcgtt gccagtcgcg ataccggcga aattctgaga tacgcgatgg
    12601 acggcacctt cttgggatgg ttcgtgccca gcggcagcgg ggggttgaca aggcccgaga
    12661 gtatggtctg gggtcccaac ggaagcctgt tcgtgtgcaa cctgaacact cacagcgtgc
    12721 tcgagtttcg cggcctcgac ggttcgcccg tggaccatga tcccggacgc ccgggtgtcc
    12781 aggcggcgtt tgccgtcggc gtcggttcgt tgagacctac gggcttggca ttcctgtatg
    12841 ccaatgagtg caacggtaac ggcgtgcccg aaaagtgcga cattgcctcc ggagccagca
    12901 ccgactgcaa tgagaacaaa tggcccgacg aatgcgaagg cgattccgac ggtgacggtg
    12961 tcatcaacgg ctgcgatgaa gacgacgaca acgacgggat ccccgatgac ggtaatcgca
    13021 gcggctatgt gggcgataaa ccctgcaccg gcggcgtgct gacaaagtgc gatgataact
    13081 gtccttacga tccgaatccc gatcaggctg accgtgacgg cgacggacgt ggcgatgcct
    13141 gcgatgtgac cgtgtttgtc gactcgcgag ccaccggggc gaacgacggc agtgattggg
    13201 ccaacgcctt caccgatctg caggatgctc ttgccgccgc cgcggattcg gatggacggg
    13261 ttgatgaaat ctgggttgcc gagggcgtct accgtcccga tcgaggtacc ggcgaccggt
    13321 catcgacttt catgctggtt ccgggagtgc ggatctatgg cgggtttgcg ggaggagagg
    13381 tgagcgttga gcaacgccgt ccgctcctgc atcccacggt gctcagcggc gacttggcgg
    13441 ccaacgatac gtccgcgttt gaacccgcca gggcaatcga caactgctat cacgtggtca
    13501 ccagcccttc ggcagtgctg aggggcaggc cggcactcct ggacgggttt gtgattaccg
    13561 gcggttgggc ggacggtcat cctccaaatg atcgcggcgg gggcttgctc gtcgtcaata
    13621 gcggtcccac cgtggcttgc tgcgaattca gaatgaatta cgcccgaaag tccggcggag
    13681 ccgttttcag cggctactac ggcaaccccg ccattgccaa ttgcagtttc cttatcaacc
    13741 atgcaggcga aggagggggc ctgtgcacgg tcttcgagag caacgcgcag atcacgaact
    13801 gcctgttggc gggcaacagc gccggacaat tcggcggagc catacacagc gaggacagta
    13861 gccccactat cgtgaactgc acgatcgcgg cgaaccacgc acgcctgcga ggcggcggaa
    13921 tcagcatcga tcgcggcaac ccgatggttg ccaataccat cgtctggggc aactcgttga
    13981 ccgcctcctc cggcgccggt tcgcagatat tcagccccaa agggggtgag ttcgtcgcct
    14041 acagttgcgt tcaggacgac gtgccgggcg acggtcgcac ctatcgcggc gtccagaaca
    14101 tcgacatgga tccgctgttt gttcggccgg ccggcgacgg cggtgacggc tggggcatcg
    14161 gcggtaatga cgatcccggc aatctgcacc tgcgcagcgg ttcgccctgc atcgaccggg
    14221 cgaacacact gatgttgacc gagaacatga gcggtctgag cggcagcggc gttatgagcg
    14281 ggcaggtgct gctcgatctg gccggcttcg agcgaatcgt ggacgcccct gacagcgagt
    14341 ccccggcgtc ttttcccggc gcgactctgg acttgggagc tcttgaaaag caccccgact
    14401 gcaatgcaaa cggcattccc gatgcgtgtg acgcaagctg tggcacgccc tacgggccct
    14461 gcgatgtccc gggttgcgga accagccagg attgcaccgc cgacaggatt cccgacgaat
    14521 gccagcccga caccgatgac gacggtctgg ccgacgtctg cgaatggtct tatggcgatt
    14581 tcgacctgga cggggacgtg gatcagagtg attatggcct tctgcagcgt tgccttggtg
    14641 atcccgcttc cgttcccctc gacccttcct gcaggggtgt tgacctcgac cgagacggga
    14701 aagtcaaccg tagcgacatc ctggtttttg tgcgctgcct tgtcggcccg gactgtccgg
    14761 cggaccccaa ctgcttgaaa taagcatctc gcgggtgcgc cgcctcgttt aaggtggggg
    14821 aggaacgatc gccccgaccc cgctacgaca cctctgccgt ctgatgcaga catgatcggg
    14881 cttgttccgt ccggctgagt agttgccgga cgagagtttg ctcggcggtt ccctcggcgc
    14941 accccgacgt gtatcgtcga gcgatttcat cgaccatttc cgggtcggcg actgtgaggc
    15001 gatcggcatc ttcacagata gcggccacgc ggcccagcga ctcaaccgcc gagtcagcgc
    15061 aggtctcgca cgtcagccgg gcgatgtcct cgaaccgacc ctcgcggtgc catcgtctca
    15121 gcaacccgaa ccacgcctgg aattgagccg gcacctccgg cggcgtcgag tcacaggccc
    15181 aagcctgcat ctgcgagcct ggcagttgcc ggagcaattg cggatcttgg ccttcaatca
    15241 ggaccagatg cattccgcgc gaaggcgaaa accgcagatc ggtcgaggcg ttcacgtgct
    15301 cacgaagact acgcgccaga ctgagcatga tccagttcag catcgccagc ggtttgcttt
    15361 ctccggtcag caccaaccgg cggccttcaa gaagcatcgc tgccgccggc cagatccacc
    15421 caaccgcttc cggcgaggcc aagtcgcgtg ttccgggcgg caggacgagc ggctcgagat
    15481 gcggaggcgg tttgagcagc ggtccggcct cgccaattac cttgccgatg gccgcgtgca
    15541 ccgcgccggg gttgctgtca aacgacctgt atgcagcctc gtccagcagg gccatgtgcg
    15601 tatagacgcg ctggccgccc cgaccggtgt gctcaacgcc ggcgtggcag acgtagccca
    15661 cgcagaaccg cctgctggtc agcctgtagc tcagcaggcc caccgcgtcg ggcctgcttt
    15721 catacagact gccgtgggaa ggcgaccgag cggtgatctc cgctttttcg tcggctcgca
    15781 ctccgcggct ggccgcgacg atgcggtatc cctggcccgt cggcgtgcgg atcgaggtga
    15841 aaacggcctg gtcgcacacc acctgtgaag cggcggacat tgcgtcacct ccgtgtgaaa
    15901 cctgctacag ttggtcgacg acccactgga agggctccag gataccctgc agcgccgtgt
    15961 ggagcggcac cggaatcacg tagtcttcct gcggggccgt ggcgtagccc agcgagccga
    16021 ccacgctgca cgcgaaaaac tcgaagtttg aaaggcggct ctcacagagg ttccacaacc
    16081 ggttcatgtt ggccttcaca aacttgcgcg ggtcgtcaaa gcaatcgggg cactggtcgg
    16141 ctttgcacag cagaatggcc gtcggcgttc cgatacgttg gttgcgaccg gcgccgtaca
    16201 tcgtgtcaat atagctcagc atcctcaccg cgaagaagtc gggctgcgaa gagccgttgg
    16261 cggccagggc cgcgtccacc agcagcatca accccgccga cttgttcagc aggcttcgga
    16321 tgaccttgaa ggtattaggc gccgccacct cggcggccag cgattcgccc gcgatgtcgg
    16381 gcatgatcag atccacccac ttgggttgcg gggaatgctt gtatacctgg tagtacgccc
    16441 agtaccactg gttggcttcc atcggcgtct tcggtggaaa catccgctgg gacatgttgc
    16501 tgatgaccgt ctgttgaaga tcgatcgagt atgccccttt ggggatcgcc tcaaagtcgc
    16561 cggcccgatg ggtcagcatg tcgagcagaa agcccaggta gaccgttttg cccacgttgg
    16621 tatcgccgat gacgctgacg atttgcggct cgcggtcctg ggcgatcgag tcgtgtacca
    16681 gggccatcgg ggcggcgcac gacagacact gcggcgtctg agcggcattg atctcttcgc
    16741 agatcatgca gcggttgggt gcgatggtcg ttgtcccttg gcctttcatt gcggcctcct
    16801 ttcaggatcg ttggacggtc cttgggcggg ctgtgccggc gtcgtccgcc cggccgtggg
    16861 cagatccggg gacggcggtg tctgactgag aggctgatcc ttggcgtcca ggtccaatac
    16921 gcaacgaaag cccacattat gcatccgagc caggctggcg agccccgtgc ggaagcaact
    16981 cgtcgcttgt gaggcgaaat aagtgtcgta agccccgccc cggatttcct tgagcagcat
    17041 gtctcccacg acctgtcggc cgcagtcgtc ggtgatctcg tagttcgacg agttccattc
    17101 ccagacgttg ccgatgagct ggagcacccc gttgggtgcc gccccgttct catattcggt
    17161 caccggcagt gtgccgccga tgcccgacgc ccagatgttg cacttggcag tgtccagcga
    17221 atctccccat ggatagcggc gtagtacgtt cgccgaactg cgaatgcgcc agcttgccgc
    17281 catctgccat tccgctccgg tcggcagccg ataacccgcc cacagagcat acgcggcggc
    17341 ctcgtagtag cagatcccca cgaccggatg ccggctgaaa cgtttgtcgt gccggccgtc
    17401 ctgccaataa cgcggtccgg gcttgcccgt ctggtccttg aagtcgatga ggtgcggcca
    17461 gatgtcttgc ggccagagct gcagatcctc atatccgccc gaatcgacga acttctggta
    17521 ttgctcctgc gtgacgcagc agcgggcgag gaggaaaggc ttggtctcga agtcctcctc
    17581 gggactgccg gggaaatcgt tcaccgccag gctgaccgat gcgaatccct cgggaaccag
    17641 ggcgaattgc tcatcgatgg cttcgacggc cgcctgaaac accgactgga tcatcgcatg
    17701 atcctgccac tgatcgcggt tgcgaacgat cgcgccgtat cgctccatct gaagcagccg
    17761 ttggagggga tcctgatcga gcttgagcgg ctcggccggc tggtaatcat cgctcctgcg
    17821 gtgcgatggc agccctcggt tgttttgtgg ctgccggttc ttccttctgc tcagtagcgc
    17881 caatcccata gtcgtaccct tctcaagtgg ctcatgcggc ccgacggccg gtcatgcgcc
    17941 gattgccttc cgcttggcat ccctgcagag tttctggaac cgttcgatgg attcgcgggc
    18001 ctcctgttcg gccaccgacg gaacgttgcg attcggagcc ttgccttgtg caagctcggt
    18061 ttcggacagc ccatactgcg gcagcagatc gttcttctgt tgagacacct tctccagttc
    18121 ctgttggagc tgggtctgga gctgggcaag gctgtctcgg gccgccgcgg cctcctgcat
    18181 ccggccgtcc aactccttct gcatgcttgc aacagttgcc tcccgctcgg cgaccgcctt
    18241 ggctttttcc tcgatcgaag ccgccagccg gttcatttcc tcctgtttcg ccttgattgc
    18301 ctcctgctcc ttgaccagtg ccgcctgctg gctcttgagt tgttcttcca ggccgcccag
    18361 cgacgtccgc tgtttctccc aatcgctgcg ctccttctca aagttgctcg tcgcttccgc
    18421 cagcgaggcc ttcgcggatt caacctgctt cagatctttc tcgatctcgg cggcccgcgc
    18481 ctcgatcttg gccacttcgg ccttgaactg ctgttcctcc tggtccaaag cggccttgcg
    18541 actgttcaac gcggcttcgt cccgcgagat cgcctcggat ttggaacgca cctgctcgga
    18601 ctgcgtgctc aactccctgg tgagcgcatc cagtcgggct tgggcttctt tcagttcctg
    18661 ctcgcgcttc tgacattcgg cctcgcgagc cgccagggtc ttctcgcggt tctcgtactg
    18721 ttcgcagagc tcctgagcct gctgatcgtg gttcttcgct tcctcggtca ggcgatgggc
    18781 atagtcttca agggccttgc ggcacgattc cagaacctgg gcgcgtgctt ccagcagctc
    18841 gcggacggtc tgcatcgcgg atacgaggtt ctgttccgtc gactcgatcc tgctcaagtc
    18901 gaaggagggt accgcgttcg gtatcgcgga ccgggtttca gtaaccggct ccgtggcttc
    18961 cttttctttt gctctggatc tcagcatgtc ttatcttccc cttcggaacc acgacttctt
    19021 ggaagagtcc gctttcgcgt tggtccgaga tgcctggtac tgttccagga gttcgcggac
    19081 gctcttgtta tctcctctca tgcgccgcat caccctgatg gccttggcgg tctcgggatc
    19141 gagggacgcc agcagcattt catcttcgtc cgcctgttgt ggcgattcgg tctctgtcgg
    19201 agcgttcgca gaggggttgg ccgggtcaac gtacacgcgt atgtgcttgc tgaccgcatc
    19261 gctgctccag tcgaccggcg gatggggcgg ctgattcgcc ggcaaggcgg tctccggcac
    19321 ctccgacgcc ggtgccgaag tctttgcctg gtccgcggtc gtgacttgag tccgcgcccg
    19381 ggcctccgat tgctgttcct ccacgggtcc ggccgttgcc cctgactggg gctggttctc
    19441 tggttggctg tgcgaggccg ctcgggattc caggtccgct tggagccggg cgcattcttc
    19501 ggccttggtc tgcagctctc ggcggacctc ggcctgtatc tcagcttgct ccagaatcca
    19561 ggcccgcaac tcgccaagca cggctttcca agaggcaagt ccggcgtcga ggtcggcgaa
    19621 tgccttgcgc agggcctcat cgggaggagg cactcgaact tcctggatgt ccttcaccct
    19681 gctgtccatc ggtccccaat cccacttgag cgaagctccc tgcacgcgaa accgacgttc
    19741 agagtgcctc gttcgccagg cagttaaact ggtgactcta ggaaatatac gaaacgttct
    19801 tgctcggcgg gacctaattt gggttatttg ttttccggtt gcctgagggc acgaaaaccg
    19861 tgtgcggggc aagggtgctc ggtcggcagt tatcgggaca aagaaacgga atactttcgg
    19921 accctttcac caccgccgga tgatcgggcg tccagcccgt agtcccgagg aggcagcgtc
    19981 tggaacctta cggccgagca tctgtgcggc ttgcgcgcat gtttccggat cacagggcac
    20041 tcaggttgcg gcctgcaaaa aggcggtccc ccttggcttc ctctgaggtt ctttggcgtt
    20101 gcgcggctgg gtgattggtt gagcctgttc taacgactga taacgtttca acgtctcgga
    20161 atgccgcact atccatatta tcttccttcg ccgctttcct gagcggcact tatatcgtaa
    20221 atctgtcttg cgttgtgctg gggacgagag accccccatg tgaggtctcg cacgcatcgt
    20281 gctgcgatgg atgcctaaag gatcagccaa gccggcccgg tgtaagctga aaggttggga
    20341 ctgagatgac ggaatgggaa aaggaatttg cgaagaagat ggagttgctc cacgaacaat
    20401 caaccacgtg tctggagcaa tttctgcaag aagatctgca agctgccttc gactccatca
    20461 gtgccttcct ggcgcagtgg aagttccaca cgtccactcc ccagactcaa gcgggtaggc
    20521 ggtcattcaa gttcggcttg accgaggacg cttatgtcat cgtcacattc cgcctggagg
    20581 gcgtcgatgt gctggagtgc gagaccgagt acggtctgcc tgccgtaggc agagttaccg
    20641 gaacgcggag tacctcgagc ctgcgaaagg ccgatcaacc gtgggccgaa agctgtttcc
    20701 agaaggctct tgacggactg cttgtcaaac tgggcgagct ggacgccagg aaaaagggtc
    20761 gagagcctgt tctggtcgca tagtccgtgc tggtttctta caggactctc atccgccggc
    20821 aaggctcttc ggtttgccgg ccgcgaatga cgccggctgg ttgacaggga gtcagcgatg
    20881 ctccctgtgt tccccgcagg gctattcggg tttcttctgc atacgcggtc gtttgaccca
    20941 cgtgcgaccc ctgtttgggg ctcaaaactg cttctgacgg ctacccgaac gcgtgcaatt
    21001 tccactcggg cttccaaaaa gccgcttccg catacctccg gtgatttctc gcgagtgcca
    21061 cgcccgcagg gcgccaatag ggaccgctaa cgaggtcctt cgtgtcgcgt atccgagcgg
    21121 gcggtcgtca accccgttcc ttggctaaca aggacttgat ggggcggctc gcacgttctg
    21181 tcagataatc tagaatttta ccgtttcgcg ggaatgacct tgcctttgaa ggattggcca
    21241 gatacgatag cggtagaacc tcagcgtaca cctcaatctt tgggagagag aggtcaacgc
    21301 cgagacattt gaacgtggac cgagcaggcc ggcttgtgca gcggaccgtg tctatggaac
    21361 gcgcagggct tctaaagttc ggcggtttct gcgttcagcc cgctccgtcc ggggggtccg
    21421 aatcgccgcg aagggggcag atggttcccg tttcgtcggt cgaatcaggg gggtggaatt
    21481 cccatcgtac cggaggagtc aatgcgagaa gataaccgcg gtggcagggt cctctaagaa
    21541 gcggcatgct gacgcggata ccttgtgtga ccggttcaga agagacggcg aggggccaag
    21601 aaggcagtgg cgcgcaatcg gcttcgcgtc acaacaagag gtgcagttct tatgagaata
    21661 cgcatgtcac gtgtcaggac cagggtcgta agcgcagcga tggcggcaat gcttgccgtg
    21721 tcgccgatcg cggtgatctc gatgtcaatg gttgccggtt gcggcggcgg aggttgctgc
    21781 ctcccgacga ttccatgcaa atgcgctttc cagttcgcgt cgggcttgct cgcgatctgg
    21841 ttgctccgga ataacggact cggagcggac ggcatctctt gctgggatct caacgccaac
    21901 ggcacctgtg ataccgcgac cgaggacgtc aacgccgacg gcgtctgcaa cgccctcgat
    21961 tgccagggtc ttgcgggcga gccggggcaa tccattcccg gaattgacgg tctcaactgc
    22021 tgggacctga acgccaacgg cgtggccgat ccggatgaag acatcaacgg cgacggcgcg
    22081 tgggatgcgt acgattgccg cgggaccgca gggcctccag gcagcgacgg caaggacggc
    22141 aagaacggca gcaacggtcc gccggggcct caaggaccga cgcggttcga tctcttcatc
    22201 gaggatttct tcacctacgg ccggatcacg gacgacgtcc gcggccaggg agaagtaatc
    22261 aacatacagg aaccgattct cagcgccgag tacagctcgt tcggcccgat cggtttccgc
    22321 gtgtccgttc cggagatcta caccgagggt aacccggtga cgttgcggct gttcttctgg
    22381 cgcgaagcct ggtcttccgt tggttgcacc accttgcggt tcgacgtttt ccgattgata
    22441 ccgggcgggg gaatcgagcg atatggagcc aatccggtct ggctgcggct ggagcccggc
    22501 agcatgacca atccgggcat gctggtggtc gatctgccat tgaatacggc accggacggc
    22561 ctcggtttcc cgaacgacct ctacgcaccc cagatgctgg cgtttgagat cagcgtcttc
    22621 ggagttggcg acacggtcta cccgccggat cttgacgggg aatacacagt cctgggggcg
    22681 gagatcttcg agtcgcttag tgccgcggat accggccttt cgggcgtcac cgtcttcacc
    22741 agcagtgccg gcgtaggaag cttctgcgga gccggcggtg gagacctcta ggatcaagga
    22801 ctacagggga acggcggcgt gtgacatgtt gacccagaga gggttgaatt catgaaaatg
    22861 atgccgcgaa tcaaggggaa ggttgccggc ctgaccatgg ccgcactgct ggcggcttca
    22921 ccattgacac tgatctcgat gtccatggtc acaggctgcg gaggcggagg ctgcctgccg
    22981 cccataccct gcaagtgtgc ctttcagttt gccgccggca tgctggcgat ctggttgctg
    23041 aggggtggtc cggacggcgt cggagcagcg ggccttcatt gctgggacct taatgctgac
    23101 ggaacgtgcg atccggcgac cgaggacgca aacacggatg gcgtatgcga cgtcaacgat
    23161 tgtcgagggc cggacggacc cgacggccag ccgattcccg gtgtggatgg tttgcagtgc
    23221 tgggatctga acggcaacgg aatcggtgag gcagaagagg acgccaacgg cgacggtctg
    23281 ttcaatgcac tggactgtcg cgggccggcc ggacctccgg gcaacgacgg tgccgacggg
    23341 acgaacggtc agcctggtcc tccggggccc ccaggtcccg atctgttcga ctggttcatc
    23401 gaagacttct tcacctatgg cgaaatcagc aacagcgtca ccggccaagg ggaggtgatc
    23461 aatatccagg agcccattct ggagtgggat gaccaaatcg gtgtcaatcc cattgccttc
    23521 cgcacggcga taccggaaat ctatcatgac ggtaacccgg tgacgttgcg agtattcctc
    23581 tggcgtgagg cgacggaagg ccggtgcacc gtgttgcggc tggacatctt ccgcgcgctg
    23641 ccgggcctgg gaattgagcg ctacggggtg ccgctctggt tgcggctcaa tgccgccggg
    23701 ctgacgaatc ccgatatgct ggttttcgac ctgccgctca atgtcccggg gcccaagggc
    23761 gtcggctttg cgaatgacct ctttgccgca caaatgctgg cctttgaact ccgggtgttc
    23821 gggtacggcg aaggtgctgc cttggatgcc cggtacacgg ttctgggtgc ggaactcttc
    23881 gagtccgcca gccctgcgga cgtcatgctg aagagggtga ccctgttcac cagcgagccg
    23941 gatatcgata aggtctgtaa cgtctgtgac cccaattcgc catggcctgg gcagtgcgat
    24001 gacggcaatc cggagacgct cgacatctgc caaccgctgc aggagggcgg ctggggcgag
    24061 tgttataata tcgtcgtcgg cccgtaagcg ggtgtacgga ccgaaatgtt gaaagagagt
    24121 gatgcgcagt ctgaggcact ggaacgaatc cgggtctcag cggtatccaa gagaaagtgg
    24181 aaggagagag agatgaggag gatcgtatta ctgacagcat gcgtgtgcct ggctggggca
    24241 gtgtcttcgg cccacgcact catgctctac ggcgtgtcgt acgacgagca gatatggaag
    24301 atcgaccgtg caacgggcgc gatcgagctg tacaagaaca cccccgggct caggtggttc
    24361 ggggcgaccg acggtccgag cgatgagtcg ttttttgcga caggcgacgg cggctcgttg
    24421 ttcaagatcg acattgcggc cgacacggtc accgcgatcg gaacttacgg cgggtccgtc
    24481 cggttgagga gtctggccta tgcggatcct gtgtcgcctg ccaccgaggg catcttgtat
    24541 ggctccgatt atcagaacct ttacaccatc gatcttgtca ccggcgtcgc caccctcatc
    24601 ggacccatca agccggtcgg cgagctcttc gtcggagtct ggtcgatgga ctatgatccg
    24661 gtggcccagg cactcttcat caccaacctg gtgaacaatc ccatcacgca tgcaatcacg
    24721 acgaagctgt atacggtgaa tccggccaat gccgaagcaa cgcttgtcgg agagatcgcc
    24781 ggcgcacttc cgattgctga catctggtat gaccgcgact cgggcaagat gtttggtgtc
    24841 actcaggtga acgggcaatt gtatgagatc gacacggcca cggccggggc gacgcccatc
    24901 ggcaccgccc ctggcgagaa catccttggc cttgccggtg cgatgatccc cgagccgggc
    24961 atgctcatgc tgctgggact cagcgtgctg ggcgttcttc gggccggccg gcgaagcgtg
    25021 cggtgagtga gttggactga cgatcccggt tgctcatccc gagccgaaag ggcatcgcag
    25081 agaaataccc gcggcgaaac ctgcgggtgc gacgtcatca tcaatcagta ggtgtacgcc
    25141 caggccagtc ggtccttgcc agggagcatc gaccggctgg ctctttttgt gcgggtacca
    25201 gacgcccgaa ctctttttga gcccggcaag ccgtgtgccc cgttcagcgg acgcagcgag
    25261 gcaagaccag gtattctgac accgtaggtt tgcccgaaag tctgccccgc catggttgcc
    25321 acagcgacca gggccggctg ctctttggcg ttcaggcttc ctccttcgtc atgttccggc
    25381 cattgagcct catttgtctg ggatttcgtc cttggccctt tgattcgtcc cgccggcccc
    25441 atcccggggt tttgaatcag ccgccgcgac cgtatactgc tctcgacgtg tgtatccgtg
    25501 aagatggtct ctggagaagg tgaggcggat ggctgaatgg cgggtctacc ccaagctggc
    25561 ccagttgaag gatgcccaag agcttattgc tcgctgggcg gaactgggat acgacattcc
    25621 gtgtgacggg cggattctga cggcggccga aggctcgccg ctcgccgagc cgatgaccgt
    25681 gttcggccgc cgcatcggca accgctggtg catccagccc atggaaggct gggacgggac
    25741 gctcgatgga agaccgtcgg aatggacgcg gcggagatgg gaacggttcg ggaagagcgg
    25801 tgcaaagctg atctggggtg gagaagcggt cgcggttcgc cacgatggac gcgctaatcc
    25861 gaaccagcta tggatcaact caaacaccca gggcgatatc gcggctttgc gcgaagtcct
    25921 cgtcggtgct caccgcgagg tcacaggcgg cagcgaggac ctgctgatcg gtcttcagtt
    25981 gacccattca ggccgtttct gcaagcccaa cgacaacacg cggatggagc cccggatcgc
    26041 ctaccatcat ccgatcctcg atgcgcgttt cggcatcaag ccggatgatg actcgatcgt
    26101 gttttccgat gacgaccttt ggcggctgat ggatgacttt gtcgcggcct cgagactcgc
    26161 ggccggggcg ggtttcgatt tcgtcgacct caagtgctgc cacggatacc tcggccacga
    26221 actgttgggt ggccatacgc ggccgggagc cttcggcggt tccttcgaga atcggacgcg
    26281 attcatccgc gagctgatcg gccgcgtccg ggctgaagtc cccggccttc acatcggcgt
    26341 caggctgagc gcttttgacc tcgtcccctt tgtcgacgat ccggcgactc gatccggcag
    26401 ccgcaaaggt gtcggaatcc ccgcaagatt cgaccattgt ctgccgtatc ggtacggctt
    26461 cgggatcaac cccgacaacc cgctcgaata cgacctcagt gagccgatcc gttttctcga
    26521 gatgctggcc ggcctggatg tcactctggt caacatcacc tgcggcagtc cgtattacaa
    26581 cccgcatatc cagcgccccg cttatttccc gccgtcggac ggctatcagc cgccggagga
    26641 cccgctggtg ggcgtcgccc gccacatcga tgtcgttcgt cagttcaagc agcggtttcc
    26701 tgagatgatc cttgtggggt cgggctacac gtaccttcag gagtttctgc cgctggtcgg
    26761 ccaggccgtc gtgcgcaagc gatgggtcga tttcgtcggc atcggccgca tggtcttgag
    26821 ttaccccgag atgccgatcc actcgcttcg caaaggcgaa ctcgacacgc gacggatctg
    26881 ccggaccttc agcgactgta ccaccgggcc gcgaaagggc ctgatcagcg gctgctaccc
    26941 gcttgacgag gcgtatcgtc actcgcaagc gggtcagaag ctcaaggaac tcaagaaggc
    27001 cgaagagggg ggctgacgcg agacggagca tgtataagct ttcctggcct aaccgcgtga
    27061 ccatgacccg gattctgttg gcaggcccgt tcgtcgtggc tctgcttcac cttcaggatc
    27121 cgcaatggag tgagcttgcc cgctgggcgg cggtcgtcgt gttcgtcctg atggccatca
    27181 gcgacggtct cgacggctac ctcgctcgtc gcctcaaggc cgagagcgcc ctggggaggt
    27241 ttcttgatcc gctggccgac aagcttgtgg tgctctcctc ggtcgtgctc ctggctcgtg
    27301 agaacaccag cgttcccggc atgcagcttc cctcgacggt tgcggtgatt gccatcggca
    27361 aagacttgat catcgtcgtg ggtttctgtc tgatctacat gattacctcc agcatctaca
    27421 tcgaggccaa acgcctgggc aaatggtgta cggccaccca gttgttcatg gtcattgcta
    27481 ttctgctctc gccgaacgtt ccggggccgc tgaaatgggg gccggtgctg ttgtggtggg
    27541 cggcgtcact tctggcgatc gccaccgtca tccagtactt caggatgggt ctctacttca
    27601 tcagccgcca cgagtcccag aaggccgcct gaacgccgaa ctggaccgtg gcgttgcttt
    27661 tcggcgataa tgggggcggg gaggtgacca ccatggcggc acccacgccg aaactccgtc
    27721 ggcgcgacat actggcggcg ggggcatttg catatgggct gctgagcacc aaacgcaagc
    27781 tcgtcgccgc gccgccggaa cacgtcattg tcgacgtcga gcagttgcgc gaagtgagcg
    27841 tcgatacgac cgccgagccc aaggagcagc actcgtgcga tattcttgtc gtggggggcg
    27901 ggctcggggg ggtggcggcg gcgatcaccg ccacagatgc cggtagagag gtggtgctcg
    27961 tggaggagac tgcctggctc ggcggccaag ccgccagtca gggcgtcagt gccctcgacg
    28021 agaacggctg gatcgagacc agcggcggta cgtgctcgta cctggcgttt cgcaaggcgg
    28081 tccgcgattg gtatcgacgc aacgcgaagc tcaagcccca agcagctcgc aaggagcacc
    28141 ttaaccccgg caacggctgg gtcagccgcc tgtgcttcga acccaaggcg gccttggccg
    28201 tgatcgacga cctgctcgct ccggcggccg cccgaaatcg gctgcgcgtg ctcaagcggt
    28261 gtaaggccta cgcagtgcgg atggaagacg atcacgtggc cgccgtggat ttcgtccacc
    28321 tcgactcggg cgatcgtttt cgcgtcaggg cccggtacgc gatcgactcc acggagctgg
    28381 gcgacttgct cgcgcttggt gccgccgaat tcgtcaccgg atttgaatcg cgtcgggaga
    28441 ccggcgagcc gaacgctccc gacgagagcg accccgagga cgtccagagc ttcacctatc
    28501 cattcgcgat tgagcttcga cgaggcgagc ggcatttgat tccgcagccg gccgaatatg
    28561 agagaaacgc aaagcaccaa ccttacagcc tcgacatcag cagacagacc ggacgtcctc
    28621 cctacgccat gttcacgacc cggccgggga cttatggtcc gttctggacc tatcggcgtc
    28681 tgatcgatgc aagccagttc gacgatccga actatcgcaa cgatgtcgcg atgatcaact
    28741 ggcccggcaa cgacttccgc ggcggcaaca ttctctcagg cacgccctcg cagcagatcg
    28801 cgctcctgcg ggctgcgaag aatctcagcc tggggctgct gtggtggctc cagcacgaag
    28861 tcgaacgcga cgaaggcggt cgcggttacc ccgagttcaa gctgcgaccc gacgtcatgg
    28921 gctcgaccga cggcctgtcg aagttccctt atatccgcga atcgcgccgg ctgcgggccg
    28981 tcaaaacgat cgtcgagcag gaaatcacga ccgccggacc ccgggccgtt cacttcgatg
    29041 atagcgtggg aatcggtcac tatggtatcg atatccacga aaccacccgc ccgcgaaagc
    29101 tggacttcaa gccggcacgc ccgttccaga ttccgctcgg ggctctgatt ccgcggcgga
    29161 cgctgaattt gctggcgggt tgcaaaaacg tcggcaccac ccacattacc aacggctgct
    29221 accgccttca tccgatcgaa tgggccgttg gcgaggccgc cggcgagacg gcctcactct
    29281 gtttcgagcg aggtcggcgt ccgaaagaca ttctcgcgaa caaggagttg ttgcgcaccc
    29341 tccagcgccg cctcgttgcc cgcggcgcac ccatcatgtg gtatgatgac cttccggtcg
    29401 gcgacccgcg attcgagaag ctccagatgc tcccattcga gtcgccggag gtgatggccg
    29461 gaatccaggg gaaccttcac gccccggacg tatcgccagg atgatgacgc ccgaaagagc
    29521 cggatattgc tcgattcttg ctcattggcc ggattacagg gcagaaggaa tgcggttatg
    29581 tggctgcccg gcaggcggat gatgacgaag ctgtcggcga gaacatggac gagtcggctc
    29641 ctcgtcattt gggtgttgat taccgcggcg ggcgcctccg ggcaacccgc ggcttcgaca
    29701 tcgtcgccga cggacgccca gcaagattcg cgcttctctg agctgatcag gagtgccgat
    29761 cgctaccacg agtctcaagc ctgtcacctt gaggcggagg cccttgagaa agctcttcag
    29821 atgctgccgc cgcgccacgc acagaggagg gaactggccc ggcgactggc ggccgtttgc
    29881 cggtcgggca tgggacacta tgcccgagca gagcgttggc tgcagactct tgcggatgag
    29941 ttgcggcctc tgccccaggg catcgatgcc ctcttcgatc tggctgacgc gtacttcaac
    30001 cacggccgat tgaatgaggc cttgtcggtc tatcgcgaaa tcctcaaacg cattgtgcct
    30061 ccggagcctc cttacgacgt cgatgaccgc tgctggaaag cctggcgcgg tgaggatctg
    30121 tgcgagtccc ccctgggcag cgatcctttg ttcccttccg acgcttcggt cagcgggcag
    30181 gtctttcgca tatcaaacct tgcacccaag gggtcgtggg aggacatccg aagattgctg
    30241 gatgaaaacc tgcgattgcg attgatgatc cggccgggcc aggaagccga ggtttggact
    30301 tcgcttcgcg cggaggcacg aaaggcaatc agcaggctgc cggatgacgc aatgcggaga
    30361 ctcgtggccg cttatgaacg caggctcgcg gagctggccg gcggcaatga ctggcggtcg
    30421 gccgttgagt tccggctctc acatcccatt ccgcaactgg agggcgtgct cgacgcagcg
    30481 attggcgaca aacttcttga cctcggccaa cctgccctcg catcaatcta ttacggccga
    30541 gcagtgcaag cagcgggaca aaacgccgag cccatcctgc tcgcccgcga gctgttcagt
    30601 cgcatacaat caggcgaaac cataccgctc gacaatgttc cggaaatccg ggttggaatc
    30661 ggcgggcagg agcgaaccct gaggcaatgg gtcgagcatt ggcagccgag gatctcgccg
    30721 gcgccgttgt caaagccggt tctcgacatc ggtcgggggg tcatgacgcg cgtaccgatg
    30781 ctcagggcca cgtgtcccct gctccaatgg caggcgcggt ggcgcgcgcg gaactgggac
    30841 gacgtctacc cttatctcga cgagttcgtc ccctgtatcg ccgccggttc acctcggcgt
    30901 ctcctgatca acatggggga cgccatagag gccgtggacc tcacggacgg tcggattgtt
    30961 tggagcttcc tgccgcccga agaaaacccg atgcctcagc ctgggcagga gtttcaggcc
    31021 cgggatgcct acgttatgtg cggcaagagc aaaactgccg tcgtcaacgg cggccgagtg
    31081 ttctgccatc tcctctgggg gcatcacgat acaccgcgag atgccggggc cctgtttgca
    31141 ctccgagaat cggacggggc gatgatctgg agcagtctgt acgtgccgca gctcgctggt
    31201 attcagtttg ccgctgatcc ggccgtctac cggggcgttg tggtggccgt ggcatggcgg
    31261 cctcgatcgg tcatgcccat gttcttcgtc gtgggcatgt ccgccgagac gggcgaggtg
    31321 ctctggctca accacctgta cagcggcggc tcgatggtgg cgtatgacaa cgaacagttc
    31381 cttgatctgc cgaccgcgtg cggcccgccg gtgattgccg atggcgtggc ttacctgacc
    31441 acaggcgcgg gtgtcgtcgc ggcggtcgat gtgcttgacg gaaccacgct ttgggcaacc
    31501 gcgtacccgc gagcccgggc gattaaccgg gaggaatggg cggtgcgcat ggcgaccagc
    31561 cggccgcccg gcgtcgttgc ggtcttccgt gacgtggtct tgttcgcacc ggtcgacgcg
    31621 cacctgctgc tggccgtcga gcgttccaca ggccggattc gttatatgca tcggtcggtg
    31681 gatctgcggg ccatagctgc agccgacgcc gagcgggcct atgtagttga gggcacgacg
    31741 gtgcgcgccg ttcgtcccgc cgatggccgg actgtgtggg agacgcaact cgtcacttcc
    31801 ggacttgtcg gcctgcctac gcttgccgac cggggactct tgtgtcccac atgggaaaca
    31861 ctgtatctgc tggatccagc caacggaggc attctggccg agcgagattg ggatcgcgcc
    31921 caggcgtgcg gatatgtgcg cgatttcggc gattgcatgg ttggaacgtc acaggtcggt
    31981 atccacatca tcgctgatcg caggctggat gacaccggcg atcgctggct ttctccggaa
    32041 tacgagcatg aaccaccgga gttgaatgtc cgttcgcggg cgggcaactg gttgcggtgg
    32101 gcgatgccgg gaatggtgaa tcggggcgat tttgtcctga gcgacagcga tccgagtcat
    32161 tttctctgcc ggtcggagtt gctgcagatg cgggatatcg atccggttcc gacgctgcaa
    32221 tgggagcggc tttcgccgtt gccgtgggac ggccgggtcg cattcagtcc cgactgggtg
    32281 gcggtctgga acatagggga ggtctatgtc ctggaagcag ccaccgggcg atcggtgtgg
    32341 gaggatcgcc cgctcgtccg cctcggccgg ccgatcagca gggtgctcat cctggggaag
    32401 gaggtgcgtg tactgctcgg ggcggggaaa gaccggggaa ctgttcccct gtatgtgtcg
    32461 tttgatggcc ggagcggcag ggacattgtg ccttccaccg gggtggatgc actctggtcg
    32521 gaaaggcctg acactggaag gcgggtcata cccgacgggc cggaacggct catagaggct
    32581 cacgccgacg ggtatcgtta cgagttccgt gacaaccgcc ttcgagcgat cgacctttgg
    32641 gacacgcgcg agcaatgggg gagcccgaca atcatagacg acgtccgctt cattgcgcct
    32701 ctgtccggcg gcgtgttggc tttgacgcag gcgaggctgg aaggccatat ccgctttcgc
    32761 gacatggctc atatccaggt ttttgaccgt cacgacgggg aacggatcaa tgctttcaga
    32821 ctcttcaaac gatggcagca tcagtttgaa cactgtgacg accgcgtcat gatctgggac
    32881 atcaatttcc tctattgcgt gcaacctccc attgcggctg cgcatggcgg ggagcaggtc
    32941 acgatccgcc agtcgcgacc cgaccctgac gcgattgccg ctctccggct ggcaagggac
    33001 ctcgagacgc ctccccaagt aagcatcggt accttgcaga ggcctccgca tgtggatgca
    33061 gagttctccg aatggtcgac ggtggaacct gtcgaactcc gcgacgtgat ggactggcgg
    33121 cccgatttcg ttcaccgcag ccgatggaag agccgttttt atggcggcgg cgacgacctg
    33181 tcggccaagg tgcgcctggc atgggcgggt gattcgttgt gcatagcggc cgacgtgacc
    33241 gatgatgcgc acgttaccac tgcctgcccc ggtttgtgga ggttcgacag catcacattg
    33301 caaatcagcg aaggaggcag cgaggatgta gatcctctga tgctcaccgt cggcagcgtc
    33361 aacggcatcg tgcggcttga aatgagcagt ccggtcatga tgctggccgc ttccgacccg
    33421 cccgggacgg agcggcattg cgagccgggc attcgtcgct tcgcgctgtc tcggccgggt
    33481 gcagcgggtg cagggggacc tgaggcaacg ctgtatgtgg aggcggcggt gcagagggac
    33541 gacttctcac gacgcactcg atacgaaatc gcgatgccga ggcagttgct tcgttatggg
    33601 cccgagtcgt actgggatct gctgatcaac gataacgacg gccagggccg cgagggcgct
    33661 ttgcagctcg cctcttcaat ctggagcatc gaggaatcgc aagcggcctg cctgcgcggc
    33721 gggcgctgac ccggactgtc ttccgtattc cctcagatct gtatcttgcg cgccatcgtg
    33781 atgtgctcct gccggcggat ttcctcgacg acatcatcac cgacgtttct gcggacgcgg
    33841 atcaggaaca tcgacctctt gcgctccttg ctgtgcgaaa actcaaagct gtcgatctgg
    33901 atcttgtgct tgtccagcag cgatgagatc ccactgacca cacccaaccg gtccttgcta
    33961 cggatggcca ggaaggtgcc ctcgggctcc acctcgaact cgaagccgtc cacgaggctg
    34021 attctcggat gcggaccgct gaagaccacg ccgccgatgt tgaactctcg atcctggccc
    34081 ttgaagccgt atcggacttc actctcgtag tcggtgaagc ccggatcaca gatcgcctcc
    34141 accttaaggc cgatgcttcg tgctcggtcc tcggcattga cgtagctgac gtaatcgagc
    34201 gagtttttca agaagccttt caggaacgcc agccgcagca gcgtgcagtc gtgccgggcg
    34261 atgctgcccc ggtaggtgat ctggagtcgt tccggaaccc agtccatgaa ctgagcagca
    34321 aaggtgccca gtcgctcggc caggaccgta tacgaggtca tgatgtcgcc cccctccaac
    34381 atccgaatct ggggcatgtt caccgggtaa tcgacgatcc cgcctcgcag ggcgcgcggt
    34441 acctgcacgg cgatgctctc ggccacgcga atctgggcct cggtcgtcga agccccgatg
    34501 tgcggagaca tcaccacgtt cggcagatcg cggaaaggat tctcgtcggg cggctcgcgc
    34561 tcccacgtgt caatgccggc cccggccacg tgaccggact tcagggcatc cagcagcgcc
    34621 gactcggtga cgatgccgcc gcgggccgcg ttgatgatga ccacgccttt cttcattcgg
    34681 gcgatctgct cgcgcccgat cattccggtc gtttcccggg tcttcggtgt gtgcaccgtg
    34741 ataatgtccg actcgcggat caatctgtcg agatccgcct tctcggcctc gtgccgctcg
    34801 aagacctcgt cggcgatata agggtcatac gccagaacgc gcatctcgaa accgcgagca
    34861 aaccgggcga cgcggtgtcc gacgttgccc aagccgatga tcccgatggt cttgcccatc
    34921 agctccacgc cctgaaagcg atggcggtcc cagcccaggt gctgcatcgt cgcgtgggcc
    34981 tcggtcaact tgcgggtgac ggccagcagc agtgcgatgg tcagctcggc cgctgagttc
    35041 gtgttcttgc cgggcgtatt gaccaccaga atgcccttcc tggtcgcata ctcaacgtcg
    35101 acattggtca caccgacggc ggcaaccgcg atgaccttca gctccttggc ggcgtcgatc
    35161 agctctctct tcaccggcgt ttccgatcgt acgatgatgc agtggtaagg ggcgatgatc
    35221 tgaaggatct cgccgatggg catatcggga tggaaatcca cctgcaggtc aggctctttc
    35281 gaaagcctct caacgccgtt tgagtgaatc ttgccggtga tcagaacacg gtacatgctg
    35341 ccttacctct cggtcgcacg tagcgacaaa actccgggcc ggccgtctcg cttcgagtca
    35401 attgctgaac cccgcgccga aatcttcgac actgggcgag gcattctaac ggaaaggtcg
    35461 cggtgctgtc gccgcccgat ccggccatgg ttgcattctg gaatgtgtgc aaaacggtag
    35521 cgcgtggtcg cagccttgcg aagtcccgcg gacgaaacag ggcccagacc tgccggccct
    35581 ggctgatgcc cgcatcggcc tcggagcaac ctcagatcgg cggccggccc gtggcaacca
    35641 ctgccggccg cttgtcgtgc aaacacgtcg cacggggtgc acgtcatggg gcttggcctt
    35701 caagcgcgtt cgtgatggcc tcgattcgat agtttccggg gccgttgccc agatccagct
    35761 caaccgtgtc acccacgcga aggccgcgca gcttcatgca caccggtgcc tggtagttga
    35821 agattcctcg ctcaacgtcg gcctcccaag gcccgaggat gctcatctcc cgccggtcgt
    35881 ttccgtcgac ggcctgcagc gtgactcgcg ttccgatgcc gacctcttcg gttgatatgt
    35941 cattgaccgt cagcagacgc ccgatggcca gttcgttttg tattcgcatg acgcgagcct
    36001 gcaggaggtc gcgttcctca agtgcgaact tgtactcgga attctcgctg agatcgcccc
    36061 gcgcggccgc ctcgccgatg gccttggcgt tctcggggat cttgaccagg cgtagatgat
    36121 tcagctcctc ctcgcgtttt ttcattccgg cctgcgtgca gtagatcgta ttctcgtccg
    36181 tccatgggtc gaccctcttc ttcggcgaga tcagctccgg atgggtgtga acgatgatct
    36241 tgagaagatc ggcgtggacc acctcgccca gaccgtcaag ccggtcgatg gtgcggtgca
    36301 cggtcgaagc gagtttcggt tccatctcgg aaatgacctt ccgaaaacgt gcatacttgg
    36361 cggcgctcaa ggccgcgcgg atctcgctcc tggcgtgtct gagcacttcg gcggaggtcg
    36421 tttcggagcg cgtcaggttg cccaggtggt ccagcagccg cagcagaatc tcgcgcaatg
    36481 gcatggcctc aggtatttcc accgccggtc ctcgccatag ccaacaaacg gcgtccaggt
    36541 gtcgggtgaa gtcgctggga atctgccgga ccgcctgttc gaggaggtcc tggcgaccgg
    36601 cctcgatcag tgattgggcg atcacgtcgc aggcttcccg gtgggccggc ggcaggagcc
    36661 gggcgaagat ctcgggccaa tcctggggtc gggcctgtct gaccagagca acggcccgca
    36721 agtagagcgg gtgctcctcg atcgtgccga gcagagccac gggatccaaa gctccggaca
    36781 gaatcgcctt cgccggtccg gcatcccgct cggcaaggct tgcttgctcg gccagccggt
    36841 cgagaaccag tgcctcggcc agcgcctcgg cgggggctct ctcgcgcacc agatcgatcc
    36901 gcgtttgcag gtcggcctga aaacgtgcga tgatcccggc gtcgggatcc acctgtcggt
    36961 tcctggcttc tcgaaagtac gcatccacga cggccatacg ttgatgcggc gtttccgcgg
    37021 ctgtccattg cgggagcacc tcgtcctcaa gtgtctgccc cttggcgtga taggtcagga
    37081 tggtcctcgc tttgccttcg accacgatgt tcgggcattt cttgaggtcg gtcttggcct
    37141 tgctccacca cttcttccag tcccgctccg gaatgaaacg agggctgagc agatgggcga
    37201 gttcatccga gtcaatccgg ccgcgatggc ttcgcaagat gccgagaacc aggctcgtcg
    37261 gatcgttgtc gatcatgttg cgaatcccat cgggatccat ctggagtaga acgcggaaat
    37321 cattgggttc ggccggatca taggccaggg ccagcgcgtc cgcatccatg gtctgttctc
    37381 cgtggtcggt gcggatggtg tacagccagg ccttcgcgtc cgccgcagtc acctttgcgg
    37441 catgctcgtc ggaccgcgag acgaggaaat cgccaacctt cacgttcaag cagatatcca
    37501 gcgtgcggag ggcgcggcgg accgatttgc cgccgcttaa gccggacatg tcgatgaggt
    37561 tcccaatctc cggccgatcg gcgtacactc tctgatagag ctccaccgcc gcctcgcgca
    37621 gatcatcact gtcatcgcag cggacgatca tttccctgac aagatccagc agctcccggg
    37681 gctcggaccg ctcctgcttg gtctccagcc acatccaggc cagtgcgccg gcctgctggc
    37741 ctttgccgtt gcgagtgaga accgccaggg cttcgagcat ttcttcgcga tcctcgctgc
    37801 cggattcaag catgtccatc cagaggtttt cgagagcctg aatctcgttg cggttggcca
    37861 gatcagtcag ttttggcgac aaactcgtca taaatgccct gctcccaata cgttgcggcg
    37921 acgggtcccg cgaatctcca ggagaccgtc gtaagtactt tctcctccgt cggcaatcat
    37981 atcatgggtt attcgcggac tgctgtccag cagcccaacg cgaagcgccc tgaccaactt
    38041 tgactggcgg tgcgttttac cctgccggct tccggcttgg tcatcatgtt gagtccgcgg
    38101 cgcttgggca cgccccacag gacttgcacg cgatgaaaag caagcggatc aggactcgcc
    38161 ggccttgctg aacgcctcga tatcggcttc cttgccgatc atcaccaggg tgttgccgct
    38221 ttgcagggtc ttgtcggccg gcggcatgaa ctcaggctca gtccctttgc tcttcctgat
    38281 ggcgataacc aggacgttgt attgccttct gacgtgaagc tgggcgaggg tctggccgac
    38341 aaacctcggc ggtacgccga tctccacgac gcgatattct cgggaaagcg gcaggtagtc
    38401 gagcaggtcc ggattgacga tgcggtgggc cattcgttcc gccgtttcct gctccgggaa
    38461 gatcacatcc gtggctccga cggccttgag aatcaccgcg tggtctttgc ttgacgcctt
    38521 ggcgtggatt cgcttcactc cgatctgttt gagatgcagc gtggcgagga tgctggcctc
    38581 cagatgctcg ccgagactga cgacggcttc atccgtgtcc ttgttgacga ccgactcgag
    38641 ggcttcgaag ctgcggctgt cgaggacgac ggcctgctgg acgtcatcgc gtatcgccgc
    38701 gacctgtctt tcggacatgt cggcggcaag aacgtcgcag cccattctcg ccagcgtcct
    38761 ggcaagatgg ctgccaaact gtccaagtcc gatgacgcac acttgtttca tggtcaacct
    38821 cctagccgat catcagccgc tcttcggcga gccggactgc ctcgggcttg tgctccatca
    38881 caaccagagc cgctgtcagc ggacccagcc gaccgacgaa catggtcagg atgatccaga
    38941 gcttgccgac gatcgtcaag gagggggtga tgcccgttga caacccgacg gtgccgaagg
    39001 ccgagatctg ctcgaacaga atctgctcga gacggatgtt cggatcccgc atttcggtga
    39061 tcgccagcac cataagcccg acaaggttga aaacggacgc ggtggcgatc agcagccccg
    39121 ttcggcggac caggtctacc ggtatccgtc ggccgccgat ggtcacgtcc tcccgactgc
    39181 gcagtcgtgc tctcagccgc gcggcccaga tggccagaga cgtggtcttg accccgccgg
    39241 cgcaggatcc cggtgagccg cccacaaaca tgaggaacac cagccagagc aaagccggca
    39301 ccggagactg attgaggtcc acggtgttga aacctgccgt gcgggcgctg atggattgga
    39361 agaggcagtg cccggcctgg gcaagcaggc cgggctcgga agccgccagc cccagcaggt
    39421 gcaggacaat cgcgccaccg aaaatcagga ccagcgaggt tgccagtacg acgcgggtgt
    39481 tgagtgacca gaccatcgga cgaggtttat tccttagtct cttccacgca cggccggcta
    39541 gttcaagtat gacggtgtgg ccgaggcccc cgcagataat gagcagggcg atggtggaga
    39601 ggatacccat gttgccggcc acatcctgca tattgccgct gaaggtcgag aaccctgcgt
    39661 tgcagaaggc cgaaatggcg tgaaagacgg ccataaaggc cccttcgccg gaactcattc
    39721 ctgcaggacg cgtgcagaag atgaccaagg cgccggcagc ttcagtcaaa aacgtggtcg
    39781 acagaatcca tgcgaggttc tgtcgtaagt gcacggccgc gctcttctga tagaaggcgt
    39841 cgtgcaaagc cgcctgagat cgaaaggaaa tcttctggcc ggcaatctgc atgatgaggg
    39901 ccgcgaaaga catgatcccc agcccgccga actggatcag gagcataatg accacttgtc
    39961 cgaagcggct gtagtcctga cccgtgtcaa cgacgaccag gccggtgacg cagacggccg
    40021 atgtggaggt gaacagcgcg tcgagcgggc tgacttgcgt cttcccgtgg gagaccggca
    40081 ggctcagcag aatagtgccg cacaggatca gccccgcaaa ggtgcccgcc aggaaacgtt
    40141 ccggactgtc gaggagccgg ctgaagagaa taagaccagg tggtctgagc atggtggttt
    40201 ccgccccgtg gcgcagcagg cgctttgccc cgtggcgttc cagaaatagc atgatcccac
    40261 ctcggcggca agcccggcgg atcgccggaa ggccgtcggg ggattactcg gatgtctcct
    40321 gcacctccac gacctcgatt tcaaaacaaa gcgtggcgtt cggaggaata tccggcgggg
    40381 atccggctgc accgtagccc agctcaggcg gaatgatcag cacacgagtg ccgccgactc
    40441 gcatgtcata gttcgtcagg ccttcctgcc accccaggat caggtcggtc aaggtgaagg
    40501 ttgccggatt ggtcttggtt tcgccgctgg aatcgaatat ctcgccgttt tcgtcggtca
    40561 gacgaccggt gtaatagacc ttgatggtgg aatctgctgt caccgccggg ccggtaccga
    40621 tcgcaatgtc tttgtactgc aagccgcttg ccgtggtcac gaactgaggg gcgggcggac
    40681 gcgcttcaat cgtcgcggac gtgatcacga catcctcgac gggcacgtca tcaaatgggt
    40741 tgccgttggg atcctcccgc gtttcggttg ctcctcgggc gatgtcgtcc gcaacgttca
    40801 tgccttcgac gactcggccg aaaacggcgt atccgctgcg gggattgtcc aggttggtgt
    40861 tgtcgaccag gttgatgaag aactgcgagg tcgccgagtt gagatcgctg gtgcgggcca
    40921 tggagatggt gccccggcgg ttgggacgcg aggggtcgaa ctcattgacg attggggctc
    40981 gcaacccgct tggctgcgtc agatcgggca gatagccgcc gccctggtca acaaaaccgg
    41041 cgatgacgcg gtggaaaatg gtgccgttgt agaaaccgct ctgcacgtaa gtgaggaaat
    41101 ttgccaccgt aatcggggcg ccctcctcat acatttcaag tacgatgtcg cccttggtcg
    41161 tgaccaggcg gacattgggc tcgctcacga tggtcacctt caccgtgtca ctgtcgcttt
    41221 ccccatcctc gtcggtcacc gtgagttcga acgtgtacgt gcccgtggct cccggccgga
    41281 acttggcctg tccggtgttc acgctcgtta gtgttaccga cgcccccccg gtttgggccc
    41341 acgcgtacgt cagcttcttg tcgtacagag actcgcttcc gcttccgtcc agcacgacgt
    41401 cctgattcaa caagccgacc agatcagggc ccgcatcggc gtcgacgccg gcgccaacgt
    41461 aagcgtcatt gatgatgatg tcctcctggg gaacgtggga gagcaactcc ggcgggtcgt
    41521 cgttggtgtt gcgggtcccg gtgggggcgt tcccgatggc gtcggccacg ctcatgccgc
    41581 ccgtgtcgac gacgcggccg atgacggtgt agtccaccgg ttcgcccgag cccggcgtat
    41641 agtcgagttc cgtgttgtcg gcaaggttaa tcagaaactg cgggacacct tcgctgaact
    41701 cgcgagtcgg cccatacaag gaaactcgtc cgcgcgtgtt gcgaagaccg ttggaggaat
    41761 cgttcaccag cggcttcttg acgttgcgat ccatgttcgc gtagtactgt ccgcccacaa
    41821 tccacttacc ttgccggacg tcgtggaaaa tggtgttgtt gtagtagccc gcgtcgacca
    41881 gctccttgaa cgttgtcgcg gccgacgtgt ggtttgtgaa cagttcgatg gtcaggctgc
    41941 cgcgcgtagt aaccagcttg acccgaggca gggtgagcgg ctccggtttg tcgggaccgt
    42001 cctgctgcgt acacatctgt ccaagtgtca aggcggccag aacgcagatg aagtatggat
    42061 ggcgggtcat tgtatctcct cttgagtcaa gaagacctct ggcaaaacgc gtattataat
    42121 aggcgtcaac cgatgttccc aagcgcgggg ggcgggcagg tgggaggcgc aaggccttca
    42181 ctggtcggga gttgtcgaga cgggtgccga tctcgggacc gaccgtccag caggcgaccg
    42241 gggacgcgat cgaggctgca agcgtgtcgc acgtggcgta tatcgggctt ggggccaatc
    42301 tcagggatcg ccgggcgaac atcctggccg caaccgactt gctgcgccag gcaccgagag
    42361 ttgccagcgt cagtctgagt tcgctgtacg agaccgagcc ggcaggggga ccggccggcc
    42421 aggggatgta tctcaacgcg gccgcccgcg tggagaccga tctggatgcg agcgccctgc
    42481 tggcggtgct tctggacatc gaggctcggc tcggacgaca acggtctgag aagtggggcc
    42541 cgcggacgat cgacctggat cttctgctct tcgacgatca ggtcatcgat acgcccgcac
    42601 tgactattcc ccatccccgc atgcacgagc gacgcttcgt gctcgaaccg ctggccgaga
    42661 tcggcggcaa cgccgtccat cccgttctgc ggttgaccat ccagcaactg ctggatcggc
    42721 tgccggagca ggtgctgaag cgagacggtc cagcctcccc gcttcggtaa gtgaccgggg
    42781 catgtgcgaa gcttgacccg aggcaacgcc cacacggtcc gtgcctgagg cggaccgccc
    42841 tttcgcgggc cgcagcccaa ggccactgcc atccggaaac atgcgcaagc tgcacacacg
    42901 tccggccgta aggtctgaca ggataggcct ggagtacgtt atatcaggca cagctttgga
    42961 gcgtcgttac cacctgcccg gtcgaatctt catcttccag gcgctttcgg aggtttttcc
    43021 cgctgacggg gcaggggggc agcaaccgcc cggcctttac gctggccgca acagccaatg
    43081 ccggtcagga cggccgttta ctccgcttcg tcaaaaagcg tctcatgaat ttcctcggcc
    43141 gcggcggccg aaggagtgaa gggcaggccc agatgatcgt acgccgcctt ggtggccgag
    43201 cgaccttggc gcgttcggat gacgaacccg atctggagta agtagggctc gacgacgtct
    43261 tccagcgtat cgcgctcctg tccgagcgtg gcggccaggg cctcgatccc cgccggcccg
    43321 cccccgtaag tcttgatcag cgagttcaga aacgaacggt cgagctcgtc aagaccgagg
    43381 tggtcaacct gcaataagct caaagccttt tccacgaccg ctgcagtcag cttgccgtcg
    43441 gcccgaacct gggcgtagtc gcgggtccgc ttgagcaggc ggttggcgac tcgcggcgtt
    43501 ccgcgggccc gggcggcgat gcgggccaac gcctcttcct ccgcctcgat cttgagtttg
    43561 cgcgcggatc gccgcagcac ctcggccagg tcttccctcg tgtagaactc gaagtggaat
    43621 tggatgccga agcggtcgcg catcggggcc gtgagcaggc ccgagcgagt ggtcgcgcca
    43681 atgagcgtga acggccgaag ggcaatgttg accgtccggc cgccgatccc gctatctatg
    43741 gtgaaatcaa cccggaaatc ctccatcgcc gcgtagagaa actcctcgac cgaccgattg
    43801 aggcggtgaa tctcgtcaat gaacagcacg tcgcctcgct tcatctccgt cagccaatgc
    43861 atcaggtcgc cggccttgtt gatcgccggg ccgctggtga tgcggatggt cgcgcccagc
    43921 tcgcgagcga tgacttgcgc gaacgtcgtc ttgcccagac ccggcggccc ggccagcagc
    43981 acgtgctcga gaggctcctg acgcttggtc gccgcctcca gcgcgatacg aagctgctcg
    44041 acgatcttct gctggccgac gatctcgtcg agacgctcgg ggcgaagcga ttcgttgaac
    44101 cgctcctcat ccatgctcat cgaagttgtg ctgatgacgc gctcacgggc catgctcgat
    44161 caaccctcgc tgcttcgaat gcggtaagcc agatggatcc actcgtcggg cgggtgctcg
    44221 ccggggtgaa gctgcgccgc cctggccagc caccgctgag catcggcccg ccgctcgcca
    44281 agggcgatca tcacctccat ggcgtcgcgc tgctcgggtg gccagctttc cgcggatatg
    44341 gccgcagact caagctcggt cgctgcggcc gcgaattccg tcactttgcc ctttagctcc
    44401 gcgatgatct gatccgccgc ccgcttgccg atccccggca gcctggccag gctgcgggcg
    44461 tcggatgact cgatcgccga ggccaccgcc gccacgggct gagacagcgc cttgagggcc
    44521 ttgcggacgc cgatgccctt cacggtgatg aagcgctcga agaaggcccg atcttccggg
    44581 tgcaggaacc cgaccagccg gggaatcagg ttgccgcctg cggcgctacc ctcgaggtac
    44641 tccatcatat gtagggtccg ctcctggccc tggcaggcag ccaattccgc cgccgcatag
    44701 ccgcagatca gcacctcata cccgaccccg tctcgttcga tgaccatgta tccttcatgc
    44761 acttccgaga tggtgccggt cattcgtacg atcatcgacg gcctcagtga gatagaagct
    44821 ggtcgttgtt gcttggtggc acacgagccg gctgccgtat cacgcacgcg gcacacaggg
    44881 cgatcgccaa ggcgtcggcg acgtcgggcg gctcaggtat ccggtggagt ttgagcagat
    44941 cagtaaccgc tcgttgcatc tggtccttag tggcccgacc gttgcccgtg acgtggcgct
    45001 tcacgctcgt ggccggcaga tggatgacgc ggatatcgcg ctgggccgcc tccagcagga
    45061 tgacgccccg cgcatgcgcc atcagaatgg ccgttcgcgg ccggctgtag tgtgagtaga
    45121 tctcctccac ggccaccagc tccacccgat ggtcatccag cacctcggca aagccggttg
    45181 ccagctcggc cagcctcagg gatagggctt cgctccggct gcagcggacc acgcctgcat
    45241 caagaacgcg taccgtgtct tcctcgtcat caaccaggat gacgccgtat ccggtctgcc
    45301 gcagacccgg gtcgactccg cagatgatct gtgtggtcgc agacaacgta tgccctttcg
    45361 gtaggccgcc agtcccccag gcccctggac ttctagactc tcggacgctt gggttgtcac
    45421 gccctcagcg gatgccccgc ggcttgagcc gtcgagagcg ccgcttttgt cagccggttt
    45481 gcctccccgc cgctggcgcg cgagaatatc cgccgctagg caaacccgcc aacagactga
    45541 cagtctaaag gcccaagggc ctaaatgtct aagcgccctt tcgccacgtc gttccgtccg
    45601 gacgatcctc gagcttgatg ctcaacgccg ccaaccggtc gcggatgatg tcggccaagg
    45661 caaactgctt ggccttgcgg gcctcagcgc ggacggtaat cagtatctgc atcaattcat
    45721 cgaccatcgt gtcgccggct gatacgggca cggccgggcg cttctcgaac aaccccagga
    45781 ttttgccgag ggctcggagc gtttgcacgg cctgcatggc catctgcttg gtttcagggt
    45841 cgccgcccga atcgagtttc tccgtgtcga tgaatcggtt gacggccgtc gccaggtcaa
    45901 acatgacacc cgtcgccgca ccagtgttga agtcgtcgtc catcgcctcg atgaagcggt
    45961 cgcgatgttc ctggcactgc ttcacgaagg cccgggccga cgcgctcttc gtctccggag
    46021 aagtagcctc gaggcctggt cccccggcgt aaggatcgct ccccgtgatc cgctggatgc
    46081 gatcgaagag ccgatagaac gtgtcgagcc ccttcttctt ggcggccagc tcctcatccg
    46141 aaaactcgat cgggcgccgg taatgggtgc tgagtacgaa gaaccggagc atctctcccg
    46201 agtgcttcga gaggagattg gtcagggtca actgatccat cagccggcgc atctccggat
    46261 ccgacttgct gatcttcttg gtgttgaacc gcgtcaggcc gttgtgcatc cagtaacgga
    46321 cgaacggctt gtcgaagcag gtctccgact gggcgatctc gttttcatga tgggggaaca
    46381 tcaagtccat gccaccacca tggatgtcga acgattcgcc cagaatcttg gcgctcatcg
    46441 ccgagcactc gatgtgccag ccgggccggc ccttgcccca cggcgagtcg aactgcacct
    46501 ccggcggttc gcccggtttg gtggctttcc acaacgcaaa gtcgcccgga ttacgcttct
    46561 cgccgctcgc cagttcgcgt ccgcctgcct gttggtcttc cggacgccgc cgcgtcagct
    46621 tgccgtagtc cgcgtccttg gaaacgtcga aatacacgtc accgcccgac acataggctg
    46681 cccctttatc gatcagccgc cggcacatgg caatgatgtc agcgatagtc tcgctggcct
    46741 tgggcatgtg gtcgatgccg gtgacgttaa gttgccgcag ggcgtcaaga tagctggcgg
    46801 tgacgcgttc ggcgagctcc atcactccgc acccctgggc tttggcttcg tcgatgagct
    46861 tgtcatcgac gtccgtgatg ttcacaaccc acgtgacctc gtagcccttg aattgaaggt
    46921 accgcttgac cgtatcgaag atgatcggtc cgaccgcgtg tccgatgtga ctcggcttgt
    46981 agaccgtcgg tccgcagacg tacatcccaa ccttgccggg ctgaaccggt tcaaacggct
    47041 ccttactctg cgtcagcgtg ttgaagactc tcaatggcat agcaggtgtg ctctcgaata
    47101 ccgatcgttc gttaccgcgg cggctcgccc ctcgcttgag ccggcgggtt catcccgccg
    47161 gtttttccgc gttccttaac gaccgagggg atcaacccct cggctcgtca agattgtcct
    47221 gacgagccgg cgggttcatc ccgccggttt ttccgcgttc cttaaagacc gaggggatca
    47281 acccctcggc tcgtcaagat cgcggtcaga gaccgcttct acgttctcct cagcccaaca
    47341 acaacagtgc aggcaatggc ctccccccgg ccgacagcat cgacgccttc gttggtcttg
    47401 gccttgatgc tcaccgcctc tggcaccagg cccagcagcc gggcgacctc ttcccggatt
    47461 gctttcttgt gaggccccaa tttgggccgt tcggcgtgaa tgatcgcatc cacgttcacg
    47521 ggcacgaaac cgcgatcggc caccaaggct aaggctcgct ccaggagtcg ggcgctgtca
    47581 attcctctgt aggccgcatc cgtgtccggg aacagttcgc cgatgtccgg caggcctgcc
    47641 gccccaagca gggcgtcgat aacggcgtgg agcaggacat cgccgtcgct gtgcccgacc
    47701 ggtccttggt caaactcgat gggcacatgg ccgagaacca gccgacggcc ggccttcagc
    47761 cggtgaaggt ccgttccaat ccccacgcgg tcaatcccgg tcgctgcctg atcgttcaaa
    47821 tggccataac ctacggtcag ttcaggccgg aatcaagctg caagccccct tgttggcaga
    47881 atgttcggtc gtctctccag cccgggttgg cacggccagg ggtgctgcga tgccgatgga
    47941 aggcctgcca ctctgatcct gcgcggctat cctggcagcc ggccggtgac ccgacgctga
    48001 gacgctatcc gcccgctcgg gtggttggcc cggccatcag cgaaactcga ataccgtcga
    48061 ttctatcggg gcgggatcgg cttcaggcct tcagccacga gtcgatgttc gccagggtcc
    48121 agggctcgcg ctgcttgcgg ctgagcatgg cattggcctc ggcgtcgccg atgaactctt
    48181 cggcctgcgg gtcccacttg agcttgcgac cgagcagcat ggcgatgttg ccgatgtgag
    48241 cgatgctgat cgtccggtgg cctgtctcgg cgggggcgta gcacggcttg cgagacttga
    48301 cgcag
//
LOCUS       DAOTPW010000007        45767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_880_length_45767_cov_5.675643, whole genome shotgun sequence.
ACCESSION   DAOTPW010000007 DAOTPW010000000
VERSION     DAOTPW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 45767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45767
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_880_length_45767_cov_5.675643"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2315)
                     /gene="gyrA"
                     /locus_tag="P5316_01110"
     CDS             complement(<1..2315)
                     /gene="gyrA"
                     /locus_tag="P5316_01110"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRR83581.1"
                     /translation="MKRDDWIDPQGDLMADAPKNPPTILPIVEELQDSYLTYAMSVIM
                     SRALPDARDGLKPSQRRILVAMNDLNLGPRAQARKCAKICGDTSGNYHPHGESVIYPT
                     LVRLAQDWNMRYPLVLGQGNFGSIDGDPPAAMRYTEARMAQAAVEMLADLKLETVDFE
                     PNYDETREEPVVLPSKFPNLLVNGSTGIAVGMATNIPPHNINEIADAIVKVIDEPNVT
                     IDELMEIVPGPDFPTGGIICGRQGIRDAYRTGRGTITLRGRTHIEEKKGRTLIVIDEI
                     PYQVLRTTIKERIVDCVKGNIMPEVADVRDESDRKHATRLVVELKKDGNADLVLNQLY
                     EYTALQTNYTVMNIALVNRQPRTLNLRQMIDVFIDHRKEVIRRRTAHLLRKARQRAHI
                     VEGLLLAVGDIDAIIQLIKQSPDPPTARERLMARPLRLPENATLIRLLPEAFVRRTTR
                     EDQNLSGTQADAILSMQLQRLTGLEMEKLAEEYRKLTEEIEGYEAILRDEALVLDIIR
                     EDTYEMKEKFGDKRRTEISGQEVGEFRMEELIPDEQMIVTITRDGYIKRVQVDAYRKQ
                     GRGGKGVKGSDIKEGDYLEHLFSANTHDYLLFFTNRGRVYWLKVYDIPAMQRTSRGRS
                     IANLLHMLPDEIHKAVLPVSRFEEKFVFFATAKGVVKKTPLAAFSRPRPSGIIAMSLD
                     LDDELIGVSQTSGSDEIVLGTRDGWAVRFDENDVRAMGRSARGVRGIDLRGEDRVVDL
                     VVTNEGASLLTVCENGYGKRTQVSEYRKTRRG"
     gene            3668..4318
                     /locus_tag="P5316_01115"
     CDS             3668..4318
                     /locus_tag="P5316_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRR83582.1"
                     /translation="MSDNRYMETSVVSYRASRPSRDLLVAAHQQITREWWDSKLGRYD
                     VFISDIVIQEIEQGDAEAAQDRLTLVSSFPILAVTEGAERLAGLYMREMPLPSKAIRD
                     ALHMAIASLNGMNYLVTWNCRHIAQGWSKRRLLELNTEQGIESPVICTPEELGGEFDV
                     GRSDYTRGPRGGEQTGARGGLRPACVLSAHRPAPASVRRQTGHSAAAQAWRDGVAD"
     gene            4391..5284
                     /gene="amrB"
                     /locus_tag="P5316_01120"
     CDS             4391..5284
                     /gene="amrB"
                     /locus_tag="P5316_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRR83583.1"
                     /translation="MSIREPAVAGQFYPGSRRQCEQEIAALLAKVGEPAKFTGLAVGG
                     IVPHAGWVFSGAVAAEVIALLARQGTVETFVVFGAMHRYGGRAAGAVFASGAWETPLG
                     RIDVDEELADAVLAGSEMLAEDAAAHDLEHAVEVQVPFIQHLAPSARILPLIVPPSAS
                     APAVGAAVAEQAKALGRKAVFVGSTDLTHYGPRYRFTPMGCGPEALRWAKEVNDRRLL
                     ELVEALKSDQIVPEAIEHHNACGSGAVAAAMEAGKVMGADRAYVLRHTTSNEVARDRL
                     GESADAVGYAGVVLVKTGSVA"
     gene            5372..5911
                     /locus_tag="P5316_01125"
     CDS             5372..5911
                     /locus_tag="P5316_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRR83584.1"
                     /translation="MNKNVKWRPYGAGALLGVMATLSVLLSTKLLGKPQYLGTSTTFV
                     RAAGLIERTVAPDHVKDNAYYTKEQVKVDWQFMLVAGIFVGALVASLIDRSFKIEAVP
                     PIWQERFGNRAAVRALGGFIGGVIAMFGARTADGCPSGNGLSGLMQLSISGFISLAFF
                     FGVGVLVARLVYVRKAGAR"
     gene            5908..6435
                     /locus_tag="P5316_01130"
     CDS             5908..6435
                     /locus_tag="P5316_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRR83585.1"
                     /translation="MNEQWLGLLTGVLFGALLQQGRVLRFEKQLGALLLKDMTIFKFM
                     LSAIIVGMVGINLLASLGLIELRVRDTRLAANMVGGALFGIGWAIAGFCPGTSVGAVA
                     EGRWHAVWVILGMLVGAALFAEAYPALQANLLSWGALGKITLASVLGLSPWVVIPILA
                     VVYIVVLVWFEKKGL"
     gene            6767..7825
                     /locus_tag="P5316_01135"
     CDS             6767..7825
                     /locus_tag="P5316_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR83586.1"
                     /translation="MKVKKIQVREFTAFENVEIEFSPGLNVFIGANATGKSHLMKLLY
                     SIIKAAEQRPGRPSHVSPDDQLKEKLANVFMPDKGEIGRLVRRTRGRKKAIARLGLGD
                     WAASLELTTLGRLHLEWPGPPPAKRCIFVPSRETLAMFDGFIGTYDRREISIDETYYD
                     LCVALSAKPLRGPRRGDIASLLRPLEHILGGRVVLEGGRFHVAAADGFIEAHLLAEGF
                     RKIAGLAHLIANGSLMQNGLLFWDEPEANLNPKLVTRVAETLRTLTRGGVQVFIATHD
                     FLLSHELSLAAEYETPPRVPMRFFAFSRADKGPVTIESGETLAELTHNPILEEFAAHY
                     DREHALFRQAGAAGRKPG"
     gene            7822..8415
                     /locus_tag="P5316_01140"
     CDS             7822..8415
                     /locus_tag="P5316_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83587.1"
                     /translation="MSTTITESNLRFEFSDPWVVLKYDEHLCYRAVMEKLKGTVVNNR
                     GEEECWRTTAVDVVATRPKLLLFLEIKDFRGHRIQTKTRVGDDLAVEVAVKTRDTLAG
                     LVGGFHRNHADLTRPFVERLLTRNTEIRVLLWLETDAYAPSPVETKRQKSRQVVLSKC
                     LKQRCAWLTSRALVVNLETGASVEGMTVKSLAGAGRE"
     gene            8432..9340
                     /locus_tag="P5316_01145"
     CDS             8432..9340
                     /locus_tag="P5316_01145"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRR83588.1"
                     /translation="MTSIRQTQSGRGVFVGRVTGNEPLCREHWRFEAEFDDFPPAMPG
                     QFVQILCADPLEENRAGGAFLRRPFSIGGLRRSGKRASLSITHRVLGIGTRWLSQLTA
                     GDGISFLGPLGRPFSIDPQRPAALLVGGGVGLPPLIWLAETLAQAGKQTIAFAGARTA
                     DLLPLTRLPDARISGAEPAMAFREFADHQIPTVVSTDDGSLGAWGLIVEHFERYLNSK
                     SDSLSESAVVYTCGPERMLAAVAKTCEKRSIPCQVCMERMMGCGMGTCQSCVIRTHDQ
                     DDPEGWRYSLCCTDGPVFDGRSIVWS"
     gene            9475..10077
                     /locus_tag="P5316_01150"
     CDS             9475..10077
                     /locus_tag="P5316_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRR83589.1"
                     /translation="MSDQPDTGAQRQNEYLGVYSQGVIHLTAQVDWPDGTFVSVRVAE
                     PLPGRVADGRELGKVIIAGFGLAGRWIADIFDRHNIEYVVVETNRATIDAQRRIGRQV
                     IEGNIAEEATLRAAGIESAAILALTVPDEEAVLAATRLARQLNPSIYIVARTLHASSG
                     MQAAQYGADEVVKAEQVVARQFYEMLLRKIGKPGPASPAR"
     gene            10112..10600
                     /locus_tag="P5316_01155"
     CDS             10112..10600
                     /locus_tag="P5316_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR83590.1"
                     /translation="MSRFPFRLPSKVSLEGLQSELSNLVERWWHCGVNTGPLDGQDWA
                     PPVELTEDADCYRVTLELPGVPRSSIDVTAVGTTLQIVGDKLSATASDSASAPKVIYS
                     ERRYGGFKRLVNMPGPVRINEVAAVLTDGVLTVTLPKATGTGPQDVRIPIRTPDEAPP
                     TT"
     gene            10724..11878
                     /locus_tag="P5316_01160"
     CDS             10724..11878
                     /locus_tag="P5316_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83591.1"
                     /translation="MQRRRKFVCGCLSAAVLLTASAAGSADVREDHRAWLIRNFDEQA
                     HAILRASTTQPADNAAGGIAWGDSYVMSALVEMLAATGDERYAEMFVRLADHVLKARD
                     DQHDRRDEYRGTVKPAWGSSKYSDGRWHVWAVHTGMICEPLARFAAVVRKNPRLNRGY
                     AAKANEYLAAAQQAVAVHEPDYRPGPAKDEAHLYGAVHRTHLPLNMQNALARAWIYID
                     DATGKPDHRELIERLARFFKNRLRVEPDGALAWEYRPPLDGSGTKFEDVSHAAINADF
                     IVLCYERGIVFDKRDIDGLEKTLLTRVIRPDGSVADHIGGQSGVNAHKAQVMRWARLA
                     IYCPAVRDRLIRLRRVEPSSGGGDMLGLALLTAASSNTATQHPAYGQNTP"
     gene            complement(12082..13515)
                     /gene="ffh"
                     /locus_tag="P5316_01165"
     CDS             complement(12082..13515)
                     /gene="ffh"
                     /locus_tag="P5316_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRR83592.1"
                     /translation="MFEALTQRFSEVFQNLRGRGKITEANVAEAMRMVRTALLEADVH
                     VQVVRKFCDEVQAKAIGAQVISSLKPDQVLVKIVHDELVSLMGPVDPRIPFVTPGPTV
                     ILMAGLQGCGKTTTCGKLAKYVMARGKRPLLVAADLKRPAAIDQLEVIGGQVGAPVYT
                     ERDHAHPVKVCRNSIIEARKTDRDVVILDTAGRLAIDEELMTEIAQIEQATTPHQVYL
                     VIDAMTGQDAVNTARHFNERLELDGLILTKFDSDTRGGAALSAKTITGKPIKFIGVGE
                     KLEALEEFHAERIAGRILGMGDIVSLVEKAQQQYDAQEAARLQRKMATGLNLEDFLGQ
                     LKKMKSMGSMKDLMKMVPGLSTQMPDMEVDDSELDRYEAIIHSMTPKERRDPSIIEAS
                     RRRRIAAGSGTDPKDVSALIKSFAQVRDMMKVMSGMSMLDRMRSVGHIAKMAASGIIP
                     KIKAGSTFKKRRETQKEKRRRRKKHRR"
     gene            complement(13591..14196)
                     /locus_tag="P5316_01170"
     CDS             complement(13591..14196)
                     /locus_tag="P5316_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83593.1"
                     /translation="MAIRFACPSCRQPIEVDDEWAGQSVGCPYCRKVITAPAVSSWPS
                     DQIPMASPLRESPEPVVPASGYPVRSPAQAASAAPWALTMAITSALLSIFASLVWLGN
                     LARVLTEKAGPNATQEELAKVYSNMIASGQLPHSPLATAAAVVGTLCGIGGLVLAIRT
                     LLWQQPRRGMAIAACIICVAFSCCGGFVLLTNLAGRAAVQP"
     gene            complement(14339..14863)
                     /locus_tag="P5316_01175"
     CDS             complement(14339..14863)
                     /locus_tag="P5316_01175"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRR83594.1"
                     /translation="MASDAGVSHTTARRWLSILEASFVVFLLRPYHRNFGKRIIKSPR
                     LYFVDTGLLCYLLRIRNPEELSFHASRGPVFETWVVSEVLKLFHNHGQVPDVYFWQDS
                     NGREVDLLIDLPGTLVVVEAKSGQTVASDFLRDLTQWKATTGCLDARAVLVYGGDESY
                     TRQDVSVISWKDWA"
     gene            complement(15032..15625)
                     /locus_tag="P5316_01180"
     CDS             complement(15032..15625)
                     /locus_tag="P5316_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83595.1"
                     /translation="MGSRCPHLLVDIAVLFLTTGSVLPAFASKVSISSLPVLSSSVLV
                     ADDTTQYTVTLTASSVAGYNDITSVRILFNMTESGPDTNNGRGYMAWGATDADITNYG
                     GSWVFADATGGGRWAYRTDTWGGTTYITPLGCSVSTAGAATGATGARTVTLTFTLKPA
                     WAHNPVFNVADGRRRGSGRLRSLPGMSQRHRRHTERP"
     gene            15883..17433
                     /locus_tag="P5316_01185"
     CDS             15883..17433
                     /locus_tag="P5316_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83596.1"
                     /translation="MKKDSQVTRRDLMEKAVAMGGMAAGVGASQAAQAAEDDQDRLAG
                     DAAVNRKIEGMESDCPLVISGVIPRLGVSAGLNGPRSESGIGALMPWADRLWFVTYTA
                     HTSTTGSGTGLFYVDEHMRVHKHPASVVGTYANRFVHAESEQMIVGPHVIDTDNKVRT
                     IEALTDYRLTATMRHLKDPANKVYVLGMESHFFEVDIRTLKTELLFTLNKNLKLPDGF
                     KPHYKGGFTAGDRVIVANNTYHERDETEGVSTGGRLAEWKGRGEWDIIESTAFNDVAG
                     TPMGDHANAFATGWDRASVMLCVLNRGKWSRYRLPKGSHTHDQAWYTEWPRIREVETE
                     RFLMDMHGLFYELPRLAYGGQVHGVTPICRHMRIVPDFCSWRGLLVLAGNQVTACTGN
                     AHLVGEPQSNLWFGKTDDLWGFGKPQGWGGPWRDTPIQPGQPSDPYLMTGFEHKCLHV
                     AHRAAEPVDFVVEVDFLGNGTWAPYVQLRVDAAGYACHEFPAGFSAHWVRLRADRPCV
                     ATAQFVYT"
     gene            18191..19882
                     /locus_tag="P5316_01190"
     CDS             18191..19882
                     /locus_tag="P5316_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HRR83597.1"
                     /translation="MEVRKRSCVPSVLLGSDIVGRAAAVAMMLCCIAAAGGERGTQVS
                     GYSRHVFDVRDFGAKGDGQTLDTAAVQAAVDACADASGGIVYVPAGRYLCGTVFLKSN
                     VTLELAPQAVLLGSGRLADYSDKTKGPEYYPFFNKCLLYAEKASKICLRGIGTINGQG
                     HLYPPGDTTHRPMLVRFVNCQDITVDGLCFQASGSWCSQYIDCADIRITGLVINNRAN
                     LNNDGIDLENCRNVTISDCRLDCEDDAIALKGSAMDVAITNCLISSYCAAFRIGPESD
                     GPFKNIAMSNCVIRDTFHNGIKIQMVEGGSLDNVVFSNLVMDNVTGPISIRLAQWLGY
                     LGARQQAEHKVDKRVAPPPVGTLRNVIISNVTARVPESPPPLVLGGVTSEPSAKTRRS
                     CISITGLPGHPPENVVLSNVRIVFAGGGTASEAARRDIPELEDEYPEYYMFGTLPAYG
                     LYARHARGLVLRGVRFELEQADHRPAVVCDDVEDLELDGFRAAGKGVDELIRLIQTKQ
                     AFIHGCSVSDPVRTFVRLEGEQSGGIVLTANNVRHADQVVERAPDVQPSAVRQEP"
     gene            20154..21554
                     /locus_tag="P5316_01195"
     CDS             20154..21554
                     /locus_tag="P5316_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83598.1"
                     /translation="MSYASVLPALLLSAGSAMGQSTVAQQNSTANQVPQGLQRYDQRW
                     QNVGSGYSSLGLESVPHSLDLRFPQSTGVTRPSYGYRPEPLRTYVPSSNISRSPLQDR
                     FALPRTMQPASGLTQPPAGQTRFMVDYAAPAAASQQGRPGSPVIEPMSPIQINPVSPA
                     GPLDQSLTPYRQMPMGGSGSQTLQLPRLPWEGTAEPAPVTAAPQSGTKLLAPTPPLAL
                     PRSREDGLRLLPEGTESPLDLILQGGPGRSVERRAEAPGGSLLRPITTPPEAARPPVA
                     GGDQTPVATPVARQPEIATAATLEAAAGKHLEIAAAHLKAGEYAKAATSYDLARVVAP
                     KSPVPLLGRAVAQLATREYSSSANSLLLAVELSPDPATFRQDFNSLLPDVAMVKERIK
                     ELQADLARFDDFRLRFLLGYAKYCSGDETAGMTDMTQAVHKMPPERAAPRRLLEALRK
                     DQLSRSATTTAPAAER"
     gene            21592..22896
                     /locus_tag="P5316_01200"
     CDS             21592..22896
                     /locus_tag="P5316_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRR83599.1"
                     /translation="MASAARALGLNDMVISQVSTGPPLDDSRIRSLIEELLEARSLDG
                     RRVLAIIPDHTRSGPTGTFFRLISEMLGRRARQLDFLIALGTHAPMPDEKIAELLQVS
                     TADRLAKFTKIGVYNHRWNDPSVLKQVGTLNRRKVEELSDGMLSLDVPVLLNKMVFDY
                     DHLLICGPIFPHEVVGFSGGHKYLFPGIAAPQIINFTHWLGALITNPVINGTRNTPVR
                     SAIEAAADMVPIERSLIGYVVEGHEARGVFAGPVREAWHAATELSEELQIVYRDKPYH
                     TVLSCAPRMYDDIWTAGKCMYKLEPVVADGGTLIIYAPHIDEVSYSHGQVLDQVGYHV
                     RDYFVKQWDRFKDFPWGVLAHSTHVKGVGRYESGKEWPRVNVVLATRIPEERCRRINL
                     GYLNPDSINPADYAGREDEGVLYLPKAGEILYRLRDMSKFGK"
     gene            22991..23224
                     /locus_tag="P5316_01205"
     CDS             22991..23224
                     /locus_tag="P5316_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83600.1"
                     /translation="MLAMLYQAVSGHNWPSPDQLAILGAFSIPIVVVAGGLWYKIRKT
                     LSENDLKRTMVERGMSAEEIERVLAAHTPDRQG"
     gene            complement(23287..24576)
                     /locus_tag="P5316_01210"
     CDS             complement(23287..24576)
                     /locus_tag="P5316_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83601.1"
                     /translation="MARVYQEFTYQFPPDGSAYPEHLVDVLWEWAEYNAAQEMPAEVF
                     RPRSKAQLAALVNPAFQASLRTEERRPVRFRAFLSDLPRELTVRFVTPLLYNMTELVK
                     IAPTVDSAHRAIVVVPRTGDSDQLEIVGIRDPTLSPSERSPKRPRWEDLLGLFVKHYE
                     WTVSVFGPGSILVQTLGFPIELRHGRIRCPFPVHRIKHVRQWYREATAEGRLGDQTIA
                     SALLGQVWQSILSEVCESHRGGTFLIAPDVPVRDLPLRIKYRLDSNVLVETLQARMTV
                     EPKLSHHLYAGKEIETTALDDAHCLQRGLVRTQNLLAALSSVDGAVVLNRDLHIIGFG
                     AEITLSSPATTDEVIHFGRHPDPVGIPPPRPLLDYGMRHRSAYHFCQAVAGAIAFVVS
                     QDAAITVFCNTQRDPRREEIQADVEMYQGLTPERWLM"
     gene            complement(24715..25659)
                     /gene="rfaD"
                     /locus_tag="P5316_01215"
     CDS             complement(24715..25659)
                     /gene="rfaD"
                     /locus_tag="P5316_01215"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRR83602.1"
                     /translation="MYLVTGGAGFIGSHIVRALNDRGITEITVVDDLTQCSRFANLAD
                     CRIVDYLDRSELRGQLDAGTFTAAVKAIFHQGACTDTMETDGRYMMDNNFTFSKALLQ
                     FALTCRVPFIYASSAATYGASPVTRIDPANERPLNVYGYSKLAFDQHVRHVLPAAGST
                     VVGLRYFNVYGPNEAHKGRMASMVYQIYRQLSQTGKARLFEGSGGYGNGEQRRDFVFV
                     GDVVKVNLFFAEGPVRKGIFNVGTGRSRSFNDIARTLIKLRGGGEIEYIPFPRELEGK
                     YQSFTEADLSELHESGYTEPFTGLEEGIAQCVKAWSAQ"
     gene            26015..26389
                     /locus_tag="P5316_01220"
     CDS             26015..26389
                     /locus_tag="P5316_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRR83603.1"
                     /translation="MAGESIKIQQIKAHRQEPVNMPGAKGARIRILIGQDDGACNFCM
                     RHFEVEPGGCTPHHEHPYEHEILVLKGSGTAKTPAGDRPFRQGDVIFVPPNVEHGFCN
                     TGNEPVEFICLIPSEQNCACPK"
     gene            26506..28539
                     /gene="ligA"
                     /locus_tag="P5316_01225"
     CDS             26506..28539
                     /gene="ligA"
                     /locus_tag="P5316_01225"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRR83604.1"
                     /translation="MTKSEAEKRIARLRDEIRLHDYRYYVLAEPTISDLEYDKLFKEL
                     KDLETQFPDLVTPDSPTQRVGGMPLEGFEQVTHAVPMLSIDNTYNEAELREFDARVAK
                     GLGGEKYEYVVDPKIDGVAISLRYEEGRLVQAATRGDGERGDDITANARTIRAIPLIL
                     TADGGLLRRDIPRLLEVRGEVYWPLDDFNAFNRKRQEAGEPTFANPRNATAGTLKQLD
                     SRVVAQRKLSFTAHGFGEVDPLECDTQYELFQAFKAWGIPISPYMRRAKNVEEVIGII
                     REWDRKRGSLDYATDGMVVKVDRLDQRERLGATSRSPRWCIAYKYAAERARTKLHSVR
                     FQVGKLGTITPVANLEPVLLAGTTVKSASLHNFDQIERLGVRVGDMVYVEKAGEIIPQ
                     VVGVDIEARPPDAREIRPPARCPECDSETVRDEGGVFLRCVNPACPAQIKERLRYFCA
                     RDQMDIEGVGDVLAGQLVDSGLVREFADLYRLKDCREELIALERMGAKSADNLLAAIE
                     KSKANPLARLLAALNIQHVGVSTAIDLAEHFGSMDALIAASREDLQQVEGIGPELAES
                     IYGFLHNEQGRRTIAHLRDVGVNMTQPRKAKPGSQPFAGKTIVVTGTLEHYGRKDIED
                     LIRQLGGKPAGSVSKKTDFVVAGAEAGSKLDKARELGVEVIDETEFRRRAGAG"
     gene            28667..28915
                     /locus_tag="P5316_01230"
     CDS             28667..28915
                     /locus_tag="P5316_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83605.1"
                     /translation="MITKSFRIDEKTAQEVNRFASGLNFSQVMHQAIEAWLRERRREH
                     RDQLVRQSCRARSKAELAEFDEIAEQAGQSALELMKEE"
     gene            28917..29255
                     /locus_tag="P5316_01235"
     CDS             28917..29255
                     /locus_tag="P5316_01235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRR83606.1"
                     /translation="MARICRGDVFEWPNVSDDHPMGSKRRKWVVVSRESFNEDSPHIL
                     ACPLTSYPPQPIDISVPKTPHNRLDHDSSLMVCRITPIRKTTMGPVIAHVDHSELQPL
                     IDRLDMIVGD"
     gene            29375..31795
                     /locus_tag="P5316_01240"
     CDS             29375..31795
                     /locus_tag="P5316_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83607.1"
                     /translation="MLRIGIFFVIVLVSTDKPAVAGTRARERGEVPAGARPAPAIDSV
                     IRFALSRDGFSFTETGEILVKSAEKPDIVRLPNDHLLVVFRWQPQPEAAAVLAAVWSA
                     DDGRSWSAPRPLVFKGGGGDFGKIAGSSLALMPGGMVRLYFSLASSQDGAARLGSAVT
                     RDGFTYQTDPEVGVRCDGADVVNPLAFWVGRELHLLATPVKPAAARASMRPPPVWVFT
                     SPDGRHFKAARPMREPVTPGDVLGDREGYRMYVCERGQIRCMGSPDGMRWRLHPVICL
                     RGGSDPAVVQLKDNSYLMLYCAPLDERSARQPEKALASAGSSDEAASDLFGQTPSGAP
                     AWEAFTEADTAAEVGSDLSSPADFTEVSTTDVPDEFPPLPDFQNPVNYMSWYTANSVP
                     AAGENAWESYADILLGNREGPEWRVIGVLEQGGWKTAEPWDPAAHPDWDAAFQSADEL
                     LVKFREATADPRPYGVPPLRMTENGEYYEAPDGPGLLFENLLPHLAASRYLAKTTLAN
                     AWRLENGQIDAEKMKDAWTTVLGNAQHLEQGASTIEKLVSVAERALVQEHARSALEYG
                     VFQTADDLEAALETLQMYDVPDPHPASYVRFECAASLDAIQYVFAPTGDGSAIASDPE
                     KIKQIGGNLSTTEMIFAALLPAKTVNEAVDLLNSHYREFDELMARGYPAFRESDFAKR
                     EQQRAKSNPITRIIMPSLDRVRHMQTRAEASRRATQLSYAAHLFKARNGRWPASLDEL
                     PPEHRESVRADPFTDQDLRYRLDGDGFTIYTAGENGIDDGGIHSPSGNMNKDGNSDDY
                     VFWPPQKN"
     gene            31971..33797
                     /locus_tag="P5316_01245"
     CDS             31971..33797
                     /locus_tag="P5316_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRR83608.1"
                     /translation="MNDTKRTRWFGSALAVLSCTVSLGCAGRADSRLDAVIDQAIREF
                     EPDWQPQQGEIDRLVVLIAPMARRAGCPDDTDRLNLLTAGYFYHLVRGGSGIPAMYRT
                     DADLLPSPDTLDRADLARTASAAKCDLCVILEPPAKNGAQAGAKLIVRAASSEPPSQQ
                     FAETVANSLGGHAGPADIVAGLNAPAIRIRMAATQPATRCFAPPPHRLMAERLYRAIA
                     TFAAGRRESLAASRSTRWPQSAPSAVDLGAVRPMRPEADRIMTIVQTIRPSGDLPLEQ
                     AAWFCDMFRRTSLTDATTVYFNPQVSIEEGDVVLRGATTAPALAGTLERALKRAGIER
                     VRNEMRCLPENGRLDGHRFAAVTVSTARTYSRPSDLGNVQTQLLYGELLWLLDHRDGW
                     YLVHASDGYWGWVRQEAVRIIEPSQFESLLNSKQAAVLKTIEISGTQVPAGARLPLVV
                     QTPWDISIRTPLGEAAVVPSGSVRVIDDFATTRPRIMPALEMLYVPYVFGARSPLGLD
                     CSGMVNNLFDRSGLPIARDATQQFLSGKLVATRWYRDTIRPGDRLYFLDNYGKIFHTG
                     IAISSTHFVHSSPPAVQISSLQKGDRLYEQYWDQCFVGAKRP"
     gene            complement(33975..34679)
                     /locus_tag="P5316_01250"
     CDS             complement(33975..34679)
                     /locus_tag="P5316_01250"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRR83609.1"
                     /translation="MTPIPVSVEKFNEQAKQDVPVLEPVLKEGDIIFRLSRTPLAGGL
                     VDFSKVVAEATESDLSHAALVYRVAPDGIILVDVSPVGISRRYLVDWYQDGTWNTVVR
                     RLRPEYQYLVPLVLAEVDRLIAEDVLYDQKFIPDDDRFYCTEFIDHCFRVAGRPLAPR
                     IRIKDFPNNGLVMYIGCAIGGIDMNNEVVVAGNQRIGLFSSSMLETVIDLRTSPPTAS
                     PRPDPVLTSYRPVPWP"
     gene            35056..35289
                     /locus_tag="P5316_01255"
     CDS             35056..35289
                     /locus_tag="P5316_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83610.1"
                     /translation="MMAAFSQDDPDAADKFRSMFGPGQIDQQIRQAIHFCWMTLPADK
                     RNVDEVEHQMRRILDRALKDRRDDAQAFGIGEE"
     gene            35371..35952
                     /locus_tag="P5316_01260"
     CDS             35371..35952
                     /locus_tag="P5316_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83611.1"
                     /translation="MTESKMRPMILRAGIVVAGYFGSTFLLFILDSAITQPHPVYVSG
                     TWLTYAARNALFWALRFGVGVAVIVTVDLGVQRIHGASSKRDLRFYPTVKGLRNAVRA
                     PMPRLVMVAGLLLLGGYYALVEIAIWETSRRPHGLIWPPHRLFVVCLISWGVLWIADC
                     LRRPGGGTIVGAIAFTTWATLSTLSIAFGCLVE"
     gene            36016..36900
                     /locus_tag="P5316_01265"
     CDS             36016..36900
                     /locus_tag="P5316_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR83612.1"
                     /translation="MTECTTRSKWAAILILFLSPLIGCVTDPQARWQGPFFFIQMADP
                     QFGMFSGDKDVEKETALFSQALDHANRLRPAFVVICGDLINRPGDEGQAAEFLRVAGR
                     LDPGIPLHLVSGNHDVENTPTPAGLERYRQRFGSDWYAFNHGGCRFVVINTTLIHDPS
                     KAPEEMERQETWLRHELTDPQQPRPVHRIVFQHHPWFNKTADEPDAYENIPLSRRGRY
                     LDWLTQAGVSAVFCGHRHGDSITRHGRMEIVSTGPIGKPLRKDPSGLRIVEVYRDRLV
                     HRYYPVDVVPEKVCLAAK"
     gene            37276..37569
                     /locus_tag="P5316_01270"
     CDS             37276..37569
                     /locus_tag="P5316_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83613.1"
                     /translation="MQSHEGLREDRREASQAAALRFYEMGWEAEICYRGYRQTLEQPK
                     LRSDPPEQARREAFWDMTAMPLFGLMSPEALLKRKPDRKTSASRPLGIGFAEP"
     gene            complement(37694..38218)
                     /gene="pyrE"
                     /locus_tag="P5316_01275"
     CDS             complement(37694..38218)
                     /gene="pyrE"
                     /locus_tag="P5316_01275"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRR83614.1"
                     /translation="MTDRELFDAVAAAALLRGDFTLRSGRKSKYYLDKYLFEAEPKIL
                     RELGERFAGHISPQTTRIAGAELGGVALAASTSMASGLPFVIIRNAKKDYGTKKMYEG
                     KIEKGDLILLVEDVATTGGQVLEAAKTITEAGATVEKIVAVIDRQEGARENIEGAGFV
                     FEALMTKTDLGIDE"
     gene            complement(38447..41893)
                     /locus_tag="P5316_01280"
     CDS             complement(38447..41893)
                     /locus_tag="P5316_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83615.1"
                     /translation="MARRWTTRSMACSACLALVSAAVGQGVPVTLQWFETSWQTIEDR
                     TADAFIAGYGQVWTPPPSKAEGGTSNVGYAVFDRFDLGSPSGPTRYGTRAGFEAVVAE
                     QNKAAIGTFIDLILNHNGFSSKDTPGFEATGDYPGFVVSGGPEGHGDFHAPGASCNSS
                     PTTCWISNLADIGQEFNIAYIRHPVTSGDPQNIPAGTAYDRPSPNNRQFYSDNDLPAN
                     SIGIHPFNTSNPMAGDPVAENATGLLLRYCRWLIEVVGVDGFRIDACKHTPDWFWRDF
                     YDRHVWLRGKPDLSGAPTTPLSFGESILGNPEIAAFVCKGTTGNCNTSGGVTGNRDAL
                     DFPLYYAIHDMLSQNGFGNWSSVVNASFDGYFDGNANNGDFGVTFVENHDAGQPLPSP
                     GLLAYAYLLTRAGLPIVYYQAGEFGSVSFPRPSRSDPLGNDDSYLTTLVDVHNEYARG
                     SYLERWIDGDVLIYERDNACLVGLNDRHDNGYDQRTVQTSFTAGTRLKELTGNATDVA
                     VDPTNAIWDVITVDAGGQATIRVPRNRNVNGDWHGRGYVVYGPVNPDGDLFVTPVAET
                     IPADSPSKPNGTRRLTPIDVITADQFEVRLETVDADPLDPAEDDLALLRMDMGMDING
                     NGYIDSLDPGFVGYGYEHFLTESVSLESGGVDIGGVQKGLYRQIIDATSLSEGRHYLG
                     VIAFRSRPAGSPPIFETWRKVILIDRLAPTMTLADPPPGTPITSSMYQIAVRSPDRTA
                     TRVHLFLDQQPGTDVVALAEAGQGLATQTDRDTFARTFTGMNEGHHRLDVVAYEPTRA
                     EPGVTTITGILVQINGFDGLGDMNNDNRTNNRDIFSFVQMAGAGNQFSPAGDMNGNGL
                     VDQDDVPLFADRMYGAGLPQGPGHEATASGGSQISTGQSFFWLQEEAAFSRLGSVTNP
                     DVQLVAGRSVPLYLWFNKATAPLGFDGISFDVRLRSDDGGSAQASISIDEPPGRWYGA
                     TAGTTRTDTGGQGIDDANAFDLSNTDTLPDNPSRFATLSLTGVTPGTVKVFLCVGGLG
                     IADSGDGAVVWLGFNNGQTTPENTNIGGNVFGLCSSVPEATIHVSAPVPGDFDFDTDV
                     DQEDFGHLQECLNGSTVPQTEPRCNDASLDGDEFVDEADMRVFLSCLSGAGVPGNPDC
                     AG"
     gene            complement(42170..42853)
                     /locus_tag="P5316_01285"
     CDS             complement(42170..42853)
                     /locus_tag="P5316_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR83616.1"
                     /translation="MRNVTVVFVAAVAMAVAPLFGDYYIAGDFNGWNPAGTAMTDLGS
                     GIWQANLTGVGGRHEFKVTTGSWDQNWPGSGNSWFFGDGDGKVTITFNANDIQDGWRG
                     NWGRMGLSTDPGTWTAVGDWQGWNNANPATAMVAQGGGIYKYQQTLAPGWYQWKAVVT
                     GSWDAIGDDFRGVNANTTWFEVTAANPTAVFQVDALTGIIRVDVVPEPAAVILVLVGG
                     LLCLRRRRS"
     gene            complement(42955..44547)
                     /locus_tag="P5316_01290"
     CDS             complement(42955..44547)
                     /locus_tag="P5316_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83617.1"
                     /translation="MTQYDLSAAVLGQWNDPTTPEGGLGDCTEAPIEQLDYFATRVFR
                     GEVQINSSSGMLSGGTNPNGSEYAYNNTGTAGVTGESANAPPAPGSGLPGDPRSQTRG
                     LEAKISLADLGLTPPISGDLALKVAVLLTAPDGYVSNQTLPGIQNGNDHATNLGPRPD
                     FTTVTGTQFAQAILTPAAFFPTLDGRDIVAEFGTSNAVASQDTVTSFGDRVQAQCQPV
                     LAPGSEINQIFVQDGLDNLSIDVAVTGNLEANGNQLVVFLDTVAGGEFTLDANAGRIG
                     GMAGDTVPLEADFALVVNIWSSVAYVDLINLQTNQSIYIGSNPVNSGEGVLDNGGVVP
                     DWRVAINNMNVVGVNNRSADDPIYNPLGIQPNNAKTATTGFEISIPAYAVGSPTEGDP
                     ICLFAMITNSYAGWLSNQFLPAGLGGGRPNYNDGIDDLAAAGYSCLTTYIGPPPPGCP
                     DPRYDADGDNDVDQADFAAFQACYTGSGGGVGAGCACFDWNPATGEGDFDIDAEDYGL
                     FEFCASGPGVPADKTCDDPVEP"
     gene            complement(44644..>45767)
                     /locus_tag="P5316_01295"
     CDS             complement(44644..>45767)
                     /locus_tag="P5316_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83618.1"
                     /translation="QNVLATEIAPIISEMPCSSVGPPFAVPNLGQALATNNNGTPDDP
                     SDDLTIRDPASTGTLLDSGFEPDHAIAVDTFGGTVHVTQYDLYSASLGTWDDPGTNNG
                     VTPCFDRIESLPYYAARIYRGQVAVDSNDGTLSGGTNPNGSQYAFNNQGTQGVTATAV
                     AAAGSGLPGDPRTQTQGLEAKIHLADLGYTPPLTSDLTIRISVLLIGGGGLVSNQSLP
                     GTARGTNQTNLGYRPDYTAVAGNQYATVTRSVQAFSPNIDGMNTVSEFGLANLVASQD
                     TVTSFADRTAVCTYLTNGSELDELYLRTDGNYLYIGLTGNLEENGNSQIILLDVTDGG
                     QNVLATEIAPDGPVENCPGNGPPGAVQGLGQPLIRNVGN"
BASE COUNT         8814 a        13960 c        14394 g         8599 t
ORIGIN      
        1 ccgcgacggg tcttgcggta ctcgctgacc tgcgtccgct tgccgtagcc gttctcgcac
       61 acggtcagca gcgatgcccc ttcgttggtc accaccagat cgaccacccg gtcttcgccg
      121 cgcagatcaa tgcctcgcac gcctctggcg gaccgcccca tggcgcggac gtcattctca
      181 tcgaaccgga cggcccagcc gtcgcgggtg ccgagcacaa tctcatcgga gccgctggtt
      241 tgggagacgc cgatcaactc gtcgtcgagg tccaggctca tggcgatgat cccgctcggt
      301 ctcggtcgac tgaatgcggc gagcggcgtt ttcttgacca cgcccttggc cgtcgcaaag
      361 aagacgaact tctcttcaaa cctgctgacc ggcagcacgg ccttgtggat ttcgtcgggc
      421 agcatgtgca gcaggttggc gatcgagcgc ccccggctgg tccgctgcat ggccggaata
      481 tcgtacacct tcagccagta gacccgccca cgattggtga agaacagcag atagtcgtga
      541 gtgttggccg agaagaggtg ttcaagataa tccccttctt tgatatccga ccccttgacg
      601 cccttgccgc cgcgaccctg cttgcggtaa gcatccacct gtacccgctt gatgtagccg
      661 tcgcgggtga tggtcacgat catctgctcg tcgggaatga gttcctccat tcggaactcg
      721 ccgacctcct gcccgctgat ctcggtcctg cgcttgtctc cgaatttctc cttcatttcg
      781 taggtgtctt cccggatgat gtcgagcacg agtgcttcgt cccggagaat ggcctcatag
      841 ccttcgattt cctcggtgag tttgcgatat tcctccgcca gcttctccat ctcaagcccg
      901 gtgagacgct ggagctgcat ggacagaatg gcgtcggcct gggtaccgct gaggttctgg
      961 tcttcccgcg tggtacgtcg cacgaaggct tcgggaagca gtcggatcag cgtggcgttc
     1021 tccgggagcc gcaaaggccg ggccatgaga cgctccctgg cagtcggggg atcgggcgac
     1081 tgcttgatca attggatgat cgcgtcgatg tcgccgacgg ccaggaggag cccctcgacg
     1141 atgtgagccc gctgacgggc cttgcgcaac aaatgcgccg tccgccgacg aatgacctct
     1201 tttcgatgat cgatgaacac gtcgatcatc tgtcgcaggt tcaacgtgcg cggttggcgg
     1261 ttgaccaagg caatgttcat caccgtgtag ttggtctgga gagcggtgta ctcgtaaagc
     1321 tggttaagca ccaggtcggc attaccgtcc ttcttcagtt cgaccaccaa ccgcgtggcg
     1381 tgcttgcggt ccgactcgtc gcgcacgtcg gccacctcgg gcatgatgtt gcccttgacg
     1441 caatcgacga tgcgctcctt gatcgttgtg cgaagcacct ggtagggaat ttcgtcgatg
     1501 acgataagcg tgcggccctt tttttcctcg atgtgtgtgc gtccgcgcag ggtgatcgtc
     1561 ccgcgcccgg tccggtatgc atcgcggatc ccttgacggc cgcaaatgat acctccggtg
     1621 ggaaagtccg gtcccggcac gatttccatg agttcgtcga tcgtcacgtt cggttcgtcg
     1681 atcaccttga cgatggcgtc ggcaatctcg ttgatgttat gcggcgggat gttggtggcc
     1741 attcccacgg cgatgccggt cgagccgttg accagcaggt ttggaaactt gctcgggaga
     1801 acgacaggct cttcgcgcgt ctcatcgtag ttgggctcga agtcgacggt ctccaacttg
     1861 agatcggcga gcatttcgac cgcggcctgc gccatccggg cctcggtata acgcatggcc
     1921 gccggcgggt cgccgtcgat cgaaccgaaa ttgccctgcc cgagcaccag cgggtagcgc
     1981 atgttccagt cctgggccag ccgcacgagc gtcggataga tgacgctctc gccgtggggg
     2041 tgatagttgc ccgaggtgtc gccgcagatc ttggcgcact tgcgcgcctg ggcccgcggc
     2101 ccgaggttca ggtcgttcat ggccacaagg atgcggcgtt gcgacggttt caggccgtcg
     2161 cgggcgtccg gcagggcccg ggacatgatc acactcatcg cgtaggtcag gtacgaatcc
     2221 tgcaattcct ccacgattgg caggatagtc ggggggttct tgggagcgtc ggccatcagg
     2281 tctccttggg ggtcgatcca gtcgtctctt ttcaccgccg ttttatagcc gatctgagcc
     2341 cgaaacgcca gtctgccggt tccctgtaag tttcattgag ccaggcagtt atgtcgagcc
     2401 gataccgccg aaaagaaaac gcgaatcaaa accgcaaatg gatccccatc gcggccgatc
     2461 aagcagcccg gaattcgact ccggacaggc accagtatat acaggctaaa aacttactaa
     2521 agtatctaaa ggctaataca taacagctaa tcgcttctgt ccccgaccgc tcgcccgtac
     2581 tcgggacagg tgccggagac attgccggtg aggtcgtagc cgcaggacag gcaacgcccc
     2641 ctcgcccgtc gttgtcgaag acgtctatgc cgaccgacct tgtggcagag aacgattgtg
     2701 ggggccactc caaggacggt gatcagtacc cacgagggag cttcacctgc acagtacagt
     2761 gctccagggg gtctctcaac ggtcagactg aactgtccat gcggcctgtg ggtgaatgcg
     2821 tgccatccta cgacgctgag aggaccgttg aagccttcgg cgtaacacca acctctgtcc
     2881 gagctcaaga tcacaccttt gagcttacct tcctgtaatc gccatgctat cactgtgact
     2941 ctgagccatt gactggcgat actaaatgag actaccagaa cggcaagaag cagagcgatg
     3001 gtcgctatgc tcaagccaag ccggccgagc cggtgctcag gctgttctgc ccgcctccaa
     3061 gcattcgtga cgcatgtgct cggctctcca cgccggaggc tcctgacgag cagccgggtc
     3121 agcatgtagc cagggtacgc gagtgcacag gcgatcggca cccaagcagg caccgtcaag
     3181 ccgactcgct gtgactgact ttggctttct tcggggggca caaacagatt gccccaccaa
     3241 tgacccgggg tcggctcccc aaatgcatac gtggtatcgg ggcgccaatc gaggcggagg
     3301 cgcccacggt actcactgag gatcatcgcg gtgttccacg gtgcccttgt tgaccggacg
     3361 gcgaggctga ccggtaccag gcaactgagg atcgcaaggc cgctcaaaac gactgcagcg
     3421 aaggatgaca agatgatggc tgtccggcgg atggtacgga tttcgcgacg cgtgttttcc
     3481 atatcgtccg tgttcacgaa catcttccgc cagctcgacg ttctcgtccg ggcctcttat
     3541 cacggattgt accgcgattg gcggtccggt tgcatccgcc atcagacaaa gggataattc
     3601 atagaatcgg acgggtgcca ggggcatcga gcccgggagc cgatgacccg gccgatatcg
     3661 aacagctatg tccgacaatc gctacatgga gacctcggtt gtcagttatc gagcgtcgcg
     3721 accttcgcgc gacctgttgg tggccgctca ccaacagatc acgcgcgagt ggtgggatag
     3781 caaactgggg cgatacgacg tcttcatttc ggatatcgtg atccaggaga tcgaacaagg
     3841 agatgccgag gccgcccagg atcgcttaac actggtatcg agcttcccca ttcttgccgt
     3901 caccgagggg gccgagcgcc ttgccgggct gtatatgcgc gaaatgcccc tcccatccaa
     3961 ggccatccgg gacgcactgc acatggccat cgcctccttg aacgggatga attacctggt
     4021 tacctggaac tgccgccata ttgcccaagg ctggagcaag cggcggctgc tggagttgaa
     4081 caccgagcag ggcatagaat ctccggtgat atgtacgccc gaggaacttg ggggtgagtt
     4141 cgatgtggga agatccgatt atacgcgggg tccgagaggc ggggaacaaa ctggcgcgag
     4201 aggcgggcta cgacctgcat gtgttctgtc agcgcatcgc ccagcaccag cgtcagtacg
     4261 ccgacagact ggtcactctg ccgccgcgca ggcttggcgc gacggcgtcg cagactgagc
     4321 gcacggatct cggaacgggt tcataatcat ccaacgatcc ggaggttgct ctggcgggag
     4381 gtggtcgctc atgtcgatcc gcgaacccgc ggtggcgggg caattctatc cgggttctcg
     4441 caggcaatgt gagcaggaaa tcgctgcact gctggcgaag gtcggtgagc cggcaaagtt
     4501 caccggcttg gccgtcggcg gaatcgtacc gcatgccggc tgggtgttca gcggagcggt
     4561 cgcggccgag gtgatcgccc tgctggcccg ccagggtacg gtcgagacct ttgtggtctt
     4621 cggagccatg caccgatatg gcggacgcgc tgcgggggct gttttcgcat ccggcgcatg
     4681 ggagaccccg ctgggccgga tcgacgtcga cgaggaactg gccgatgccg tgttggcggg
     4741 atcggagatg ctcgccgagg acgccgcggc tcacgatctg gaacacgccg tcgaggtcca
     4801 ggtccctttt attcagcatc ttgccccctc agccagaatc ctgcctctca ttgtgccgcc
     4861 gtcggcgtcg gcccccgcgg tgggggcggc cgtagcagag caggcaaagg ccctcggccg
     4921 caaggctgtc ttcgtgggct ccaccgacct gacccattac gggccgagat accggtttac
     4981 gcccatgggt tgcggaccgg aggccttgag atgggccaaa gaagtcaacg accgccggct
     5041 gcttgagttg gtggaagctc tgaagtcgga tcagatcgtg ccggaggcca tcgagcatca
     5101 caacgcttgt ggcagcggag cggttgccgc tgccatggag gccggcaaag tcatgggggc
     5161 cgaccgagcg tacgtcctgc ggcatactac cagcaatgag gtcgcccgcg accggttggg
     5221 cgagtcggcc gatgccgtgg gatatgcggg cgtggtgctc gtcaagaccg gcagcgtcgc
     5281 ttgacccatt tccggcaggc gctgctgaac gaagcgttct tctgaacgga aagacgtctt
     5341 tccgagtgtt cggatttcac aaggaggaaa catgaacaaa aatgtgaagt ggagacccta
     5401 tggggcgggg gccctactgg gcgttatggc gacgctgtcg gtgctgttgt ccacaaaact
     5461 gttgggaaag cctcaatacc tgggcacttc cacgactttt gtccgggcag ccggcctcat
     5521 cgagaggacc gttgctccgg accacgtaaa ggacaacgcc tactacacga aggagcaggt
     5581 caaagtcgac tggcagttca tgctggttgc gggcattttc gtgggggcgc ttgtggcctc
     5641 gctgatcgat cgcagcttca agatagaggc cgtgccgccg atctggcagg aacggttcgg
     5701 gaaccgagcg gctgttcgcg ccttgggcgg ttttatcggt ggcgtcatcg caatgttcgg
     5761 cgcccgaacg gccgacggtt gccccagcgg caacgggctg agcggcctca tgcagctatc
     5821 catcagtggc ttcatctccc tcgcgttttt cttcggcgtt ggcgttctgg ttgcccgtct
     5881 ggtctatgtt cggaaggcag gtgcgcgatg aacgagcagt ggttgggttt gttgacgggt
     5941 gtgctcttcg gtgcgttgct tcagcaagga cgggttttgc gattcgagaa acagctcggg
     6001 gccctgcttc tgaaggacat gaccatcttc aagttcatgc tttcggcgat cattgtggga
     6061 atggtcggca tcaacttgct tgcgtcgctg gggctcatcg agctgagggt ccgcgacaca
     6121 cggttggccg ccaacatggt cggcggagca ttgttcggca tcggctgggc catcgccggc
     6181 ttttgccccg gcacgtcggt cggagcggtg gccgagggtc gttggcacgc ggtctgggtc
     6241 attctcggga tgctggtggg ggccgccttg ttcgccgaag cctatcctgc gttgcaggcg
     6301 aatctgttgt cctggggcgc gctcggaaag atcaccctgg cgagcgtgct gggtctgagc
     6361 ccttgggtcg tgattcccat cctggccgtc gtctacatcg tcgtgctcgt ctggttcgag
     6421 aagaaggggc tgtaagaggc gcgtttccac ctggcttgga gtcggcgaac ggggtggagt
     6481 cgcgtgagag ctccacacca agccattggc acccattgga gccattgtcg acttgctggg
     6541 gcacctgatg cggcgagcca ggcaggcggg tttatcgtga aacgtgccgg ccgctggacc
     6601 tcacgacatc tgcagctaga ctatatgttc tgaccaagcg agccgtcacc gatccgggcg
     6661 cgtcgcgaca cggcgttgtc agccttggag atcgcccatt gtgaacgcgc gaaggtccga
     6721 gagcttgccg cccggatgaa gctccggggg ttgctgaggt ggccagatga aagtcaagaa
     6781 gattcaggtc cgggagttca ctgccttcga gaacgttgaa atcgagtttt ctccagggct
     6841 gaacgtcttc attggggcga atgcgaccgg aaaatcacat ctcatgaagc tgctctactc
     6901 gatcatcaag gcggcagaac agagaccagg cagaccgtcc cacgtttcac ccgatgacca
     6961 gttgaaggag aagctggcga acgtgttcat gccggacaaa ggcgagatcg ggcgcctcgt
     7021 ccgcagaact cgaggaagaa agaaggcgat cgcgagatta gggctgggcg actgggcggc
     7081 ctccctcgaa ctgacaactt tggggcgttt gcaccttgag tggcccggtc cgccgcccgc
     7141 taagcggtgc atcttcgtcc catcgcgtga gacactcgcc atgtttgacg gcttcatcgg
     7201 cacatatgat cgtagagaga tctccattga tgagacctac tacgacctgt gtgtggcctt
     7261 gagcgccaaa ccgctgcgcg ggccacgtcg cggggacatt gcctctctgc tccggccact
     7321 cgaacacatc ctcggggggc gcgtggtgct tgagggtgga cgtttccatg tcgccgccgc
     7381 cgatggtttc atcgaagctc atctacttgc ggagggattc cgtaagatcg ccgggctggc
     7441 gcatctcatc gccaatggtt ctctcatgca aaacggcctg ctattctggg acgagcccga
     7501 ggcgaacctc aatcccaagc ttgtcactcg cgttgccgag acccttcgta ctctgacacg
     7561 cggcggtgta caagtattca ttgcgacgca tgactttttg ctcagccacg aactttctct
     7621 ggcggcagag tatgagactc ccccgcgggt tccgatgcgg ttcttcgcat tcagtcgggc
     7681 agataaaggc ccggtcacca ttgagtcggg tgaaaccctc gcagaattga ctcacaatcc
     7741 aatcctcgag gaatttgctg ctcactatga ccgcgagcat gcgctgtttc gacaagcagg
     7801 agcagcagga aggaagcccg gatgagcacg accatcacgg aaagcaatct tcggttcgag
     7861 ttcagcgatc cctgggtcgt tcttaagtat gacgaacacc tctgctaccg cgccgtcatg
     7921 gagaagctga aaggtactgt ggtgaacaac cggggtgagg aggagtgctg gcgcaccact
     7981 gctgttgacg tcgtggcgac ccggcccaag ctcctgctct ttctcgaaat caaggacttc
     8041 cgcgggcacc gtatccaaac aaagacccgt gtcggagacg atctggcagt ggaggtcgcg
     8101 gtgaagaccc gagatactct tgccggactc gtcggaggct ttcacaggaa ccatgctgac
     8161 ctgacgcgcc cgttcgttga acgacttctg acacgaaaca ctgagattcg tgtcctgctc
     8221 tggcttgaaa ccgacgcgta cgctccctcc ccggtcgaaa cgaaacggca gaagagccga
     8281 caggttgttc tcagcaagtg cctgaagcag cgatgcgcgt ggctcacgag ccgcgctttg
     8341 gtggtaaacc tcgagactgg tgcgagtgtg gagggaatga ctgtgaagag ccttgcaggt
     8401 gcaggacgcg agtgagcggg gcacggacag catgacttca atcagacaga cccaatccgg
     8461 ccgtggcgta tttgtcggcc gagttaccgg caacgaaccc ctttgccgtg agcattggcg
     8521 gtttgaagcg gagttcgatg atttcccccc ggctatgccc gggcaattcg ttcagattct
     8581 gtgtgccgac ccgctggagg agaatcgggc gggcggggcg tttctgcgtc gtccgttcag
     8641 catcggcgga ctgcggcgca gcggcaaacg ggcctcgctc tccatcaccc atcgcgttct
     8701 gggcatcggc acgcgctggc tgtcgcagtt gactgccgga gacgggatca gctttcttgg
     8761 tccgctcggc aggcccttct cgatcgatcc gcaacggcct gcggcgctgc tggtgggcgg
     8821 gggagtcggc ctgcccccgc tgatctggct ggccgaaacc ttggctcaag ccggcaagca
     8881 aacgatcgca tttgccggcg cgaggaccgc cgaccttctg ccgctgaccc gccttcccga
     8941 tgcccggatc tcgggtgccg aaccggctat ggcgttcaga gaattcgccg atcatcagat
     9001 tcccacggtg gtgtccacgg acgatggcag cctgggggca tgggggctga tcgtggagca
     9061 ctttgaacgg tatctcaaca gcaagagcga ctcactcagc gaatctgcgg ttgtctacac
     9121 ctgcggtccc gagaggatgc tcgccgccgt ggccaagacc tgcgagaagc ggtcgattcc
     9181 ctgccaagtg tgcatggaac gaatgatggg ctgcggcatg gggacctgcc agtcgtgcgt
     9241 catccgaact cacgaccaag atgatcctga aggatggcga tacagcctct gttgcaccga
     9301 tggacccgtg tttgacggcc ggtcgatcgt ctggagctga cgccggcgga ttgccgctca
     9361 ttgacggctg ggtgattcgt ccgcgatacc gctcgaagct cggggcacat ataatggtaa
     9421 ggcaacgcac tcaggtgggg gttgcggggc aggatacaag gcaagggcga actcatgagc
     9481 gatcagccgg ataccggtgc ccagcgacag aacgagtacc tgggcgtcta ctcccaggga
     9541 gtaattcatc tgaccgcgca ggtagactgg cctgacggga cgtttgtctc cgttcgcgtg
     9601 gctgagccgc tccccggccg cgtggccgac ggccgtgagc tgggcaaggt gatcatcgcg
     9661 ggtttcggac tcgcggggcg gtggattgcg gacattttcg accgccacaa catcgaatac
     9721 gtggttgtcg agaccaaccg agccaccatt gatgcccagc gtcgcatcgg ccggcaagtt
     9781 atcgaaggta acattgccga ggaggctacg ctgcgcgccg caggcatcga aagtgcggca
     9841 atcctggcgc tgaccgtccc cgacgaggag gcggttctgg ccgcgaccag actggcccgt
     9901 caattgaacc cgagtatcta tatcgtcgct cggactctcc atgcctcgtc gggaatgcaa
     9961 gccgcccagt acggggctga cgaggtggtg aaggccgaac aagtcgtcgc aaggcagttt
    10021 tacgagatgc tgctccgcaa gatcggcaaa ccggggccgg cctcgccggc acgctgatca
    10081 ggcgccgagc ggcgtggtga ggagatgaga catgtcgaga ttcccgtttc gccttccgag
    10141 caaggtttct ctcgagggac tgcaatctga gttgagcaac ctcgtcgagc gatggtggca
    10201 ctgcggcgta aacaccgggc ccctggacgg gcaggactgg gccccgccgg tggaactgac
    10261 cgaagatgcc gactgctatc gcgtgaccct tgaattgccc ggcgtaccgc gctcgtcaat
    10321 cgatgtgacg gccgtcggca cgacactgca gatcgtcggc gacaagcttt cggccaccgc
    10381 ttcggacagc gcctcggctc caaaggtgat ctacagtgag cgacgatacg gcggtttcaa
    10441 acgcctggtc aatatgcccg gaccggtgcg gatcaacgag gtcgccgctg tcctgaccga
    10501 tggcgtgctg accgtcacac tgcccaaagc cacggggacg gggccccaag atgtacggat
    10561 cccgatccga acgcctgatg aggctccgcc gacgacctga gactgctcca gggcggactg
    10621 cctttttgcg ggccgtgacc ccaaagcgca ctgtgacgcg gaaacatgcg cgcaagacgg
    10681 gcaccagttg cgacggaaga gacgaatcgt tgaggccgac ggaatgcagc gaagaaggaa
    10741 gttcgtttgt ggttgcctgt cggcggccgt gttgttgaca gcctcggcag ccggatcggc
    10801 cgatgttcgc gaggaccacc gcgcttggct gatcaggaat ttcgacgagc aggcacatgc
    10861 cattctgcga gccagtacga cacagccggc cgacaatgcc gccgggggaa tcgcctgggg
    10921 cgacagttac gtgatgtcgg cactcgtcga gatgctcgcc gccacgggag acgagcgata
    10981 cgcggagatg ttcgttcgac tcgccgacca tgttctcaag gcgcgtgacg accaacacga
    11041 tcgccgtgat gagtatcgcg gaacggtcaa accggcctgg ggatccagca aatacagcga
    11101 cggcagatgg cacgtgtggg cggtccacac cgggatgatc tgcgaaccgc tggctcgatt
    11161 cgcggcggtc gtgcgaaaga acccgaggct gaacaggggg tacgccgcca aggcaaacga
    11221 ataccttgcc gccgcccaac aggcggtggc ggttcatgag cccgactacc ggcccggtcc
    11281 cgcgaaggat gaggcgcatt tgtacggtgc ggttcaccgg acccacttgc cgctcaacat
    11341 gcagaacgcg ctggctcgcg catggatcta catcgacgac gcgaccggaa aacccgatca
    11401 tcgcgagctt atcgagcggc tggcgagatt cttcaagaat cgtctgcggg tcgaaccgga
    11461 cggggccctg gcctgggagt atcgcccacc ccttgacggt tcaggcacca agttcgagga
    11521 tgtgtcccac gccgcgatca acgctgactt catcgtgctc tgttacgagc gcggcatcgt
    11581 ctttgacaag cgggacatcg acggcctgga gaaaaccctg ctgaccaggg tgatccggcc
    11641 ggacggttcg gtcgccgacc atatcggtgg acaaagcggc gtgaacgcgc acaaagcaca
    11701 ggtcatgcgg tgggcccgtc tggcgatcta ttgccctgcg gttcgggatc gtcttatccg
    11761 gcttcgccgc gttgagccgt cgagcggcgg cggcgacatg ctgggactgg cgttgctgac
    11821 ggcggcaagc agtaacacgg caacccagca ccccgcctac gggcaaaaca cgccttgagg
    11881 aaggaattct cgatcgagcg gagaatcgcg gccgcaagtt gagcgaagcg aatcccaccg
    11941 tggcgggccg gtccacgctc gttgaagacc gaggggataa acccctcggc tcgtcaagaa
    12001 ttgccgctgc aagccgctcc tgctgaacct cgccacggcg agtctcgcct tcggcggacc
    12061 tgaaccccga actctgaacc cttaacgtcg gtgcttcttt cggcgtctgc gcttctcttt
    12121 ttgggtctcg cggcgctttt tgaaagttga gccggccttg atcttgggga tgatgccgct
    12181 ggcagccatc ttggcgatgt ggcccaccga tcgcatgcga tcaagcatgc tcatgccgct
    12241 catgaccttc atcatgtcgc ggacctgggc aaagctcttg atcaatgcag agacgtcctt
    12301 ggggtcggtc ccgctgccgg cggcgatacg gcggcggcgc gaggcctcga tgatgctcgg
    12361 atcccgacgc tccttggggg tcatcgagtg gatgatggct tcgtagcgat ccagctccga
    12421 atcgtcgact tccatgtcgg gcatctgggt gctcaagccc ggaaccatct tcatgagatc
    12481 cttcatggac cccatggatt tcatcttctt gagctggccg aggaaatcct ccaggttcaa
    12541 gccggtggcc atcttccttt gaagcctggc ggcctcctga gcatcgtatt gctgctgggc
    12601 cttctcgacc aggctgacga tgtcgcccat gcccaggatg cgcccggcga ttcgctccgc
    12661 gtgaaactcc tccagcgcct ccagcttttc gcccacgccg atgaacttga tgggtttgcc
    12721 ggtgatcgtc ttggcggaca gggcggcgcc gccgcgggtg tccgaatcga acttggtcag
    12781 gatcagaccg tcgagttcca gccgctcatt gaaatgccgt gcggtgttca ccgcatcctg
    12841 gccggtcatc gcgtcgataa cgaggtagac ttggtgcggg gtcgtcgcct gctcgatctg
    12901 ggcaatctcg gtcatcaatt cctcgtcgat cgccagacgg ccggcggtgt cgaggataac
    12961 cacgtcacgg tcggtcttgc gggcctcgat gatgctgttg cggcagacct taacaggatg
    13021 ggcatgatcc cgttcggtgt agacgggcgc acccacttgg ccgccgatga cttcgagttg
    13081 gtcgatagcc gcgggacgct tcaagtcggc ggcgacgagc aaggggcgtt tgccgcgggc
    13141 catgacgtac ttggccagct tgccgcaggt cgttgtcttg ccgcaaccct gaaggccggc
    13201 catcagaatg acagtgggac cgggagtcac aaaggggatg cgcggatcaa ccggccccat
    13261 gaggctgacc agctcgtcat ggacgatctt gaccagcacc tgatcgggtt tgagcgaaga
    13321 gatgacctga gcaccgatgg ccttggcctg tacctcatcg cagaacttgc gcaccacctg
    13381 gacgtggacg tccgcctcca gcaacgccgt ccgaaccatg cgcatggcct cggcgacgtt
    13441 ggcctcggtg atcttgccgc gtccgcgaag gttctgaaac acctcgctga atcgttgtgt
    13501 cagagcttca aacatgggga atatgataga cgggcgcggc ggcggtgaca aaccgtcagc
    13561 cgtcacgccc tgcgacagat ggcggctggt tcacggctgg acggctgctc ggccggcgag
    13621 attcgtcaac aagacgaacc cgccacagca actgaaagcg acacagatga tgcaggcggc
    13681 gatagccatt ccgcgacggg gctgctgcca taggagagta cgaatggcca acaccaggcc
    13741 gccgatgccg cacagagtgc cgacgaccgc cgccgccgtg gccaacggcg aatggggcaa
    13801 ctgcccgctg gcaatcatgt tagagtagac cttcgcaagc tcttcctggg tcgcattggg
    13861 accggccttc tcggtcagga cccgcgccag attcccgagc cagaccagcg aagcgaagat
    13921 cgacagcaag gcactggtga tcgccatggt gagcgcccag ggagcggcgc tggcggcttg
    13981 cgcaggcgag cggacgggat atccagacgc aggcaccacc ggctcgggcg actcgcgaag
    14041 agggctagcc atggggatct gatcagaagg ccaggaactg acggccggag cggtgatgac
    14101 cttgcggcaa tagggacagc cgacagactg gccggcccat tcatcgtcga cctcgatagg
    14161 ctggcggcag gatggacatg caaaacgaat cgccatgcga gcacttcctg ttggcacgga
    14221 gcgttggcac cgacgaaccc ggcaccaccc gcccacgcag agcaaacatc ataaaccgga
    14281 aacgcgaaag ccacaaatgc ggcccggcca acgtgaaacg ccgtggacac gcctgagatc
    14341 atgcccagtc tttccaggag atcaccgaaa catcctggcg tgtgtatgac tcgtcgccgc
    14401 cgtagacgag aacagcccgc gcgtccaggc atccggtagt ggccttccat tgcgtcagat
    14461 ccctcaagaa atccgatgca accgtctggc cggacttggc ctcaacgacc acgagggttc
    14521 ctggcaagtc gatgagcaga tcaacctcac gcccgttcga gtcttgccag aagtagacgt
    14581 cggggacctg gccgtggttg tgaaacaact tgaggacttc cgaaaccacc caggtttcga
    14641 acaccgggcc gcggctggcg tggaagctga gttcctcggg attccggatt cgcagcaggt
    14701 aacagagcag ccccgtgtcg acgaagtaca gtctgggtga cttgatgatc cgcttgccga
    14761 aattgcggtg atacggcctc agaagaaaga ccacgaagct cgcttcgaga atcgataacc
    14821 atcgtcgggc ggttgtgtgt gaaacccccg catcagaggc caagccggac agttccagga
    14881 tttgtcctga acggcccgca cagagcaaga tgctcgtcag tccgggcagt cttggatcgg
    14941 cggcgagttg ggaccgctga gacagcccag gaagacggcg aggtcctctg agtcgacatc
    15001 cgtatcgccg tccagcttcg catcttggca gtcagggtcg ttctgtgtga cggcggtgcc
    15061 gctgagacat gcctggaagt gaccgaagtc gccctgatcc acgtcgccgt ccgtcggcga
    15121 cgttgaagac cgggttgtga gcccaagccg gtttgagcgt gaaggtgagc gtgacggtgc
    15181 gagcgccggt ggccccggtg gcggcacctg ccgtggacac cgagcagccc agcggcgtga
    15241 tgtaggtcgt gccgccccac gtgtcggtgc gataagccca tcgcccgcct ccggttgcgt
    15301 ccgcgaaaac ccaggacccg ccgtagttgg tgatgtccgc gtcagtcgcg ccccacgcca
    15361 tgtagccgcg gccgttgttc gtgtccggcc cggactcggt catattgaag aggatgcgga
    15421 cgctggtgat gtcgttgtag cccgccacac tgctggccgt gagtgtcact gtgtactgcg
    15481 tcgtgtcgtc ggcgaccagc accgaggaac tgagtactgg cagactggag atactgacct
    15541 tactggcaaa ggcaggaaga acagaacctg tcgtcagaaa gagaactgcg atgtcgacaa
    15601 gaagatgagg gcaacgggaa cccatgttct ttcccctgca atcgaggaat taccttttcc
    15661 gcatgagatg cgactgtacg tattctggca aatgagccaa cgcgaatcaa ccacaatgat
    15721 cgcctgtccg gtaacaaggt tgtcgctgac ggaggcgagg ttagactcag ggggcttcgg
    15781 gtcggtctgc aggttcttcc gtgtgaacga cttgctgccc accgaggaac cggcaaggcg
    15841 ggttcaatcg ctcatttccg gtgagcaggc aggagttggt cgatgaagaa agattcgcag
    15901 gtcacgcgtc gcgatcttat ggaaaaggcc gtggccatgg gcggcatggc ggcgggggtt
    15961 ggcgcttcgc aggccgcgca agccgccgag gacgatcaag acaggttggc aggcgatgcg
    16021 gccgtcaacc ggaagatcga gggcatggaa tcggactgcc cgctggtcat cagcggcgtc
    16081 attccgcggt tgggggtcag cgccggcctc aacggcccgc gcagcgaatc gggaatcggg
    16141 gcactcatgc cctgggccga ccggttgtgg tttgtcacct acaccgcgca cacttccacc
    16201 accggcagcg gaacggggct gttctatgtg gacgagcaca tgcgggtcca caagcacccg
    16261 gcctcggtgg tcgggacata cgccaatcgc ttcgtgcatg ccgagtcgga gcagatgatc
    16321 gtcgggcctc acgtcatcga caccgacaac aaggttcgca cgatcgaggc gctcaccgac
    16381 taccggctca ccgcgaccat gcggcatctg aaagacccgg ccaacaaggt ttacgtcctc
    16441 ggcatggaat cgcacttctt cgaggtggac atccggacgc tcaagaccga gctgctgttc
    16501 acactcaaca agaacctcaa gctgccggat ggcttcaagc ctcattacaa gggcggcttc
    16561 accgccggcg accgagtcat cgtcgccaac aacacctacc acgagcgcga cgaaaccgag
    16621 ggcgtctcga cggggggccg cctggccgaa tggaagggcc ggggcgagtg ggacatcatc
    16681 gagagcacgg cgttcaacga tgtcgcgggc acgcccatgg gcgatcacgc gaacgccttc
    16741 gcgacgggct gggaccgggc gtcagttatg ctctgcgtat tgaaccgggg gaaatggtcc
    16801 aggtaccgcc tgccgaaggg tagccacacg cacgatcagg catggtacac cgagtggcct
    16861 cggattcgcg aggtggaaac cgaacggttc ctcatggaca tgcacgggtt gttctatgaa
    16921 ctgccgcgcc tggcctacgg cgggcaggtc cacggcgtca cgcccatctg tcggcacatg
    16981 cggatcgtgc ctgatttctg ctcgtggcgc ggcctgctgg tgctggcggg caaccaggtc
    17041 acggcctgca ccggcaacgc tcacctggtc ggcgagccgc agtcaaacct gtggttcggg
    17101 aagactgacg atctgtgggg cttcggcaag ccgcagggct ggggcgggcc gtggcgggat
    17161 acgccgatcc agccgggcca accctcggac ccatatctga tgaccggctt cgagcacaag
    17221 tgcctgcatg tggcgcaccg ggcggccgaa cccgtagact tcgtcgtcga ggtcgacttc
    17281 ctgggtaacg gcacgtgggc tccgtatgtg caattgaggg tcgatgccgc cgggtacgcc
    17341 tgtcacgagt tcccggccgg cttcagcgcc cattgggtga gactgcgagc cgaccggccg
    17401 tgcgtggcaa cggcccagtt cgtgtacacc tgatgcttcc gcgctgagtc cagagtacgc
    17461 ccctccctgc gagggtgccc tgctgcgcct tgccattcat gcccgtcgtt ccacctcttc
    17521 gatcccagtc gcctcagaag atcgccgact gccgcatgtc caccttctgc cgcggcaaca
    17581 gcaacgctct tgccactgtt gtcacaggca ttcgcatcgg taccctttgc tgctatgact
    17641 tccaaaacct tctctaaact cttgcgtacg gcaaactaca gaggcataca gccgtctgaa
    17701 tcctttccgt cgcaatcgtg cccgtaccaa agcagcaact cgaccatctg tctggagtca
    17761 cccgcaacac ccggtggagt cgtacggtta tgagaatcgc atctgttgac atcttttcag
    17821 atccgggggt cgaacgccgc gccgcttgtc aaggcaattg cccttggtga ggatgcccga
    17881 tgtcgacggt atgatggtcc gcttgccggc gcatggttgg ggtgtgagga aaccagcaac
    17941 tgaaggcggc aaactcgccg tcagccctca cccagagtac catgtgccgg taattgggta
    18001 tcgggcagta ggccggcaga cggcccgggc gtcgctgtca ggcctcggcc ggcacgtata
    18061 ccaaggccgc atcgttttac ggcttctgga gcagcagccg agccgccgcc caattgagga
    18121 cgcgacaaat caagccgcaa ggaacacacg agctcgtggt ggcataagct gaagggtagg
    18181 ataggggtat atggaagtca ggaaaaggag ctgtgtcccc tctgtactgc tgggtagcga
    18241 catcgttgga cgggctgcag ccgtagcgat gatgttgtgc tgcatcgcag ccgcgggggg
    18301 tgagcgcgga acgcaagtca gcggctatag caggcacgtg ttcgacgtgc gggatttcgg
    18361 cgccaagggc gatggccaga cgctggacac agcggcggtc caggcggccg tcgatgcctg
    18421 cgcggatgct tcgggcggga tcgtgtacgt gccggccggc cgatacctgt gcggaacggt
    18481 gttcctgaag tcaaacgtca ccctggaact ggccccgcaa gccgtgctgc tgggcagcgg
    18541 gcgactggcg gactactccg acaagacaaa agggcctgag tactacccgt tcttcaacaa
    18601 gtgcctgctc tacgccgaaa aggcatccaa gatctgtctg agaggaatcg ggaccatcaa
    18661 cgggcaaggg catctgtacc ctcccggcga caccacgcat cgtcccatgc tcgtgcggtt
    18721 cgtcaattgt caggatatca ccgttgacgg cctgtgcttc caggcctcgg gctcgtggtg
    18781 ctcgcagtac atcgactgtg cggacatccg catcaccggg cttgtgatca acaaccgggc
    18841 gaacctgaac aacgatggca tcgacctgga gaattgccgc aacgtcacga tctcggattg
    18901 ccgcctcgac tgcgaggacg acgcgatcgc actcaagggc tcggcgatgg atgtcgcgat
    18961 caccaactgc ctgatcagca gctactgtgc ggccttccgc atcggcccgg agtcggacgg
    19021 cccgttcaag aatatcgcca tgtcgaactg cgtgattcgc gacacgttcc acaacgggat
    19081 caaaatccaa atggtggaag gtggctcgtt ggacaacgtc gtgttctcga acctggtgat
    19141 ggacaacgtg accggtccga tctcgatccg gctggcacag tggttgggtt acctgggggc
    19201 caggcagcag gccgagcaca aggtggacaa gcgtgtggca ccgccgccgg tgggcacctt
    19261 gcggaacgtt atcatcagca acgtgacggc tcgtgttccc gagagcccgc cgccacttgt
    19321 cctgggcggc gtgaccagcg agccgtccgc caagacgcgg cgctcctgca tttcgatcac
    19381 cggcttgccg ggccatccgc cggagaacgt cgtgctgagc aacgtgcgga tcgtgttcgc
    19441 cggcggcggt acggcaagcg aagctgcccg ccgggacatc ccggagctgg aggacgagta
    19501 ccctgagtac tacatgttcg ggacgctccc ggcttacgga ctgtacgccc gacatgcccg
    19561 cgggctggtg ttgcggggcg tgcgatttga actggagcag gccgatcatc gcccggctgt
    19621 tgtgtgcgac gacgtcgaag acctggaact ggacggcttt cgcgcggcgg gcaagggcgt
    19681 tgatgaactg attcgcctga tccagacgaa gcaggcgttt atccacggct gcagcgtctc
    19741 ggacccggtc aggacgttcg tgcgtctgga aggcgagcaa agcggcggga tcgttctgac
    19801 ggccaacaac gtacgccatg ccgaccaggt tgttgaacgg gcaccggacg tgcagccatc
    19861 cgcagtccgc caggaacctt aggcaaaacg gctcaagcat cgcacttcca gcggagtacg
    19921 agcatgtcca cgcaaagccg tgggcatggt gcccgacaca acctgctgag acgggctgga
    19981 agaccgacgg gatgaacccc tcggctcgtc aaggcagcga tcggccaagt accgataaat
    20041 cggctataat agtaaaggat gcctgtttga actccgtttg ccctcagcct tgtgccgata
    20101 ccaggagggt atcacaggct accggcgccg ccgagaggtg gaacatgaga ggaatgtctt
    20161 atgcatccgt tttgccggcg ctgctgctgt ccgcagggag cgcgatgggt caatcgaccg
    20221 tcgcccagca gaacagcacc gccaatcaag tgccccaggg cttgcagcgg tatgatcaac
    20281 ggtggcagaa cgtgggcagt gggtatagct cgctcggcct ggaaagcgtg ccacattcac
    20341 tggacctgcg ttttccccag agcacgggtg tgactcggcc cagctatggg tatcgacccg
    20401 agccgctgcg cacgtacgtt ccatcaagca acatttcgcg aagcccgcta caagaccggt
    20461 tcgccctgcc aagaactatg cagccggcat ccgggttgac gcagccgccg gcgggccaga
    20521 cgcgtttcat ggtggattac gccgccccgg cagcggcttc ccagcaggga cggcctggga
    20581 gtccggttat tgagccgatg agcccgatcc agatcaaccc cgtgagtcct gccgggccgt
    20641 tggatcaaag cctgactcct tatcggcaga tgccgatggg cggcagcgga tctcaaacgc
    20701 ttcagctccc gcgtttgccc tgggaaggca cagccgagcc ggcccctgtg acggctgcgc
    20761 cgcaatccgg taccaagctg cttgcaccga cgccaccgtt ggcgctgcca cgcagccgag
    20821 aagacggcct cagattgctg ccggaaggca ccgaatctcc gctggatctg attctacagg
    20881 gcggccccgg gcggtctgtt gaacggcgag ccgaggcccc gggaggcagc ctcctccgcc
    20941 cgatcacaac gccgcccgaa gcggcccgac cgccggttgc gggcggcgat cagacgccgg
    21001 tcgcgacgcc ggttgcccgc caaccggaaa tcgcgacggc cgcaacgctc gaggcagccg
    21061 ccggcaaaca cctcgagata gccgcagccc atctcaaggc gggtgagtac gcgaaagcgg
    21121 ccacgagcta cgacctggcc cgcgttgtcg cccccaagag cccggttccg cttctgggac
    21181 gcgccgtcgc gcaactggcc acccgcgagt acagcagcag tgcgaacagt ctgttgctgg
    21241 ccgtggaatt gtcgccggac ccggccacgt tccggcagga tttcaacagc ctgctgcccg
    21301 acgtcgcgat ggtcaaggag agaatcaagg aactccaggc cgacctcgct cgctttgacg
    21361 atttccgtct tcgctttctg ctgggctacg ccaagtattg cagcggcgac gagaccgcgg
    21421 gcatgaccga tatgacccaa gcggtgcaca agatgccccc cgaacgcgcg gcaccccgca
    21481 ggcttctgga ggctctccgg aaagaccagt tgagccggtc ggccacgacg accgcgccgg
    21541 ccgcggagcg atgaccctcg ttttgacact gaatactgcc gggatgtgcg gttggcgtct
    21601 gccgcacggg cattggggct gaatgacatg gtgatttcgc aggtttcaac ggggccgccg
    21661 ctcgacgatt cgcgcattcg ctcgcttatc gaggagctac tcgaagcgcg ttcgctcgat
    21721 gggcggcggg ttctggcgat tatccccgac cacacgcgca gcgggccgac gggcaccttt
    21781 ttccggctga tttccgagat gctcgggcgg cgggccaggc aactcgactt cctgatcgct
    21841 ctgggcacgc acgcccccat gccggacgaa aagatcgccg aattgctaca ggtaagcacg
    21901 gcggaccggc tggcgaagtt cacgaagatc ggggtataca atcatcgctg gaacgatccg
    21961 tcggtcctca aacaggtggg tacgctgaac cggcgcaagg ttgaggagct atccgacggg
    22021 atgctcagcc tggacgtccc ggtgctgctg aacaagatgg ttttcgacta cgaccacctg
    22081 ttgatctgcg ggccgatctt cccgcacgag gtcgtcgggt tttcgggcgg acacaagtac
    22141 ttgtttcccg gcatcgccgc cccgcaaatc atcaacttca cccactggct cggggccttg
    22201 atcaccaacc cggtcatcaa cggcacgcga aacacgccgg tgcggtcggc catcgaggcc
    22261 gccgctgaca tggtaccgat cgaacgcagc ctgatcggct acgtcgtcga gggacacgag
    22321 gcccggggcg tgtttgccgg gccggtccgg gaggcatggc acgcggccac ggagctgtcg
    22381 gaagaactgc agatcgttta ccgcgataag ccctaccata cggtgctgtc gtgcgcgccg
    22441 agaatgtacg acgacatttg gacggccggc aagtgcatgt acaagctcga accggtggtg
    22501 gccgacggcg gcacgctcat tatttacgcg ccgcacatcg acgaagtctc atactcccac
    22561 ggccaggtgc tcgaccaggt cggctaccac gttcgcgact acttcgtgaa gcagtgggac
    22621 cggttcaagg actttccctg gggggtgctc gcacacagca cgcacgtcaa aggggtcggc
    22681 cgatacgaga gcgggaagga gtggccgcgg gtgaatgtcg tcctggccac ccgcatcccg
    22741 gaggaacgct gccggcggat caacctcgga tacctgaacc cggattcgat caacccggcc
    22801 gactacgcag gacgcgagga cgaaggggtg ctgtatctgc ccaaggccgg cgaaatcctg
    22861 tatcggctga gggatatgag caagttcggg aaatagggtc ctgggttcgg ggttcagcgg
    22921 tcagtgaaga tggccgctcg ggtgaaataa agcgatgcga cccgtgacca cgcttctgga
    22981 ggtaccctct atgctcgcta tgctgtatca ggcagtgtcg ggccacaatt ggccgagtcc
    23041 cgatcagctc gcaatcctcg gggccttttc cataccgatc gtggttgttg ccggcgggct
    23101 ctggtacaag atccgcaaga ccctctccga gaatgacctc aagcggacaa tggtcgaacg
    23161 cgggatgtcg gccgaggaga tcgagcgggt tctagccgcc cacacgccgg atcgccaagg
    23221 gtgacgccat cagtcgtttc cactcccgct ttcgcggagc cctgaacccc gatgcctgaa
    23281 gcctggctac atcagccacc gctccggcgt caatccctgg tacatctcga cgtccgcctg
    23341 gatctcctcc ctgcgcggat cgcgttgcgt gttgcagaac accgtgatgg cggcatcctg
    23401 ggaaacgacg aaggcgatgg ctccggccac ggcctggcag aaatggtagg ccgagcggtg
    23461 gcgcatgccg tagtccagca gcggcctcgg tggcggaatg ccgaccggat cgggatgtcg
    23521 gccgaagtgg atcacctcgt cggtcgtcgc aggtgaactc aaggtgatct ccgcgccgaa
    23581 accaatgatg tgcaaatcac gattcagcac caccgcgccg tcgacagagc tgagcgcagc
    23641 gagcaggttc tgtgtccgga cgaggccccg ctgcaggcag tgggcgtcat cgagagcagt
    23701 ggtttcgatc tctttgcccg catacaaatg gtgtgaaagc ttcggttcga cggtcatccg
    23761 agcttggaga gtctcgacca agacgttgga atccagccgg tatttgattc gcagcggcag
    23821 atcacgcacc ggcacgtcgg gtgcgatcag gaaggttcct ccgcggtgtg attcgcagac
    23881 ttcgctgagg atgctctgcc agacctgccc aagcagcgcc gaggcgatcg tttgatcgcc
    23941 cagccggcct tcggcggtcg cttcacgata ccattgacgc acgtgcttga tccgatgcac
    24001 cggaaacggg cagcgaatgc gtccgtgtcg aagctcgata ggaaagccga gcgtctgcac
    24061 gagaatcgac cccggcccaa acaccgagac cgtccattcg tagtgtttga caaacagtcc
    24121 gagcaggtct tcccaccgtg gacgtttggg ggaccgctcg gaaggcgaca acgtcgggtc
    24181 gcgaatgccc acaatctcaa gttgatcgga gtcgcccgtg cgggggacca cgacgatcgc
    24241 gcggtgggcc gagtcgacgg tcggcgcgat cttgaccaat tcggtcatgt tgtagagcaa
    24301 gggcgtgacg aatcgaaccg tcagctccct gggcaaatcc gacaggaagg cccgaaaccg
    24361 aaccgggcgg cgctcctcgg tacgcaggct ggcctgaaaa gccgggttga cgagggctgc
    24421 gagttgagcc ttcgagcgag gacggaaaac ttccgctggc atttcctggg cggcgttgta
    24481 ttccgcccat tcccaaagca cgtcgacgag atgctcggga taggctgagc cgtcgggggg
    24541 gaactggtac gtgaattcct ggtagacccg cgccattcgg gtgactccgg tttgtctgct
    24601 caagtaagga tcggggcatt ccctttgggc ccgatcaaac ggcccacgcc ggacttggag
    24661 gctcttgcga acagctcgtc ttcctgcgcc agggggacgg ccccgacact tttttcattg
    24721 agccgaccat gctttgacgc attgcgcgat gccctcctcc agacccgtga agggttcggt
    24781 atacccggac tcgtgcagct cggacagatc cgcctccgtg aagctctggt atttgccctc
    24841 cagctcgcga gggaacggga tgtactcgat ttcacccccg cctcgcagct tgatcagcgt
    24901 tcgggcgatg tcgttgaaac tccggctgcg accggtaccc acgttgaaaa tccccttgcg
    24961 aaccggcccc tcggcgaaaa acagattaac cttgactacg tcgccgacga acacaaaatc
    25021 tcgccgctgc tcgccgttgc catacccgcc gcttccttca aacagacggg cctttccggt
    25081 ttgggagagc tgacggtaaa tctggtacac catcgacgcc atgcggcctt tgtgcgcctc
    25141 gttggggccg tagacgttga agtaccgcag gcccacgacg gtactgccgg cggcggggag
    25201 cacgtggcga acatgctggt caaacgcgag tttggaatac ccatagacgt tgagcggccg
    25261 ctcgttggcg gggtcgatgc gcgtgaccgg gctggccccg tacgtggccg cactggaggc
    25321 atagataaat gggacgcggc aggtgagggc gaactgaagc agggccttgg agaacgtgaa
    25381 gttgttgtcc atcatatagc ggccgtcggt ctccatggtg tcggtgcagg cgccctgatg
    25441 gaagatggcc ttcaccgcag cggtgaacgt gcccgcgtcg agttgtccgc gaagctcgct
    25501 gcggtccagg tagtcgacga tccgacagtc ggccaggttg gcgaagcgcg agcactgggt
    25561 caggtcatcg accacggtga tttcggtgat cccccggtcg ttgagggccc gcacgatatg
    25621 gctgccgatg aaacccgctc cgccggtgac cagatacatg ttaccatcgc tccccagcgc
    25681 ccgtaagatc aatcagcgtc aacttatcgg tgacggggac aagatgcaag aaaaccgatg
    25741 ccttgaagca gccgcaatcc ggcgaaggcg tccaaacccg tgtcgtacct accagcctcc
    25801 gagccgcaat gccgaaccgg cgtacctcgc aacgatgatg gccgaggctt ggacgcgaac
    25861 gcagtgtcgc ggctgcatca agggtgattc gtatcgccgt ctgtgatatg atgcctcccg
    25921 ccgacgtagg gattgtgaaa cggcaaagtg catttgccgg gctgttgcca aagatacgcg
    25981 ggggaaggac ggacagcgaa gggagaaacc gatcgtggca ggcgaaagca tcaagataca
    26041 gcagatcaag gcccaccggc aggagccggt gaacatgccg ggggccaagg gggcccgcat
    26101 ccgcatcctc atcggccagg acgacggggc atgcaacttc tgcatgcggc atttcgaggt
    26161 cgaacccggc ggctgcaccc cgcaccacga gcatccgtac gagcacgaaa tcctggtcct
    26221 caagggtagc ggtacggcca agacccccgc gggcgaccgg ccgttcaggc agggcgacgt
    26281 gatcttcgtg ccgccgaacg tcgagcatgg attctgcaac accggcaacg aaccggtgga
    26341 gtttatctgc ctgattccgt cggagcagaa ctgtgcgtgt ccgaagtaac ttgaagacag
    26401 ttgtcagtct tcagtcgtca gtactcagtt cagacatcca aaccgggctt ctgactgaaa
    26461 actgagaact gaccactgag aactccaatc aggtccggtt gcctcatgac caagtccgaa
    26521 gccgaaaaga gaatcgctcg tcttcgtgac gagatacgcc tgcacgacta ccgctactat
    26581 gtgctggccg aaccgacgat ctccgatctg gaatacgaca agctcttcaa ggagctgaag
    26641 gatcttgaga ctcagtttcc cgacctggtc acgcccgact cgccgacgca aagagtcggc
    26701 ggaatgccgc tggagggttt tgaacaggtc actcacgccg ttccgatgtt gtcgattgac
    26761 aacacctata acgaggcgga gcttcgagag tttgacgcgc gggtggccaa ggggctgggg
    26821 ggagagaaat acgagtacgt ggtggacccg aagattgacg gggtagccat ttcgctgcga
    26881 tacgaagagg gccggctggt gcaggccgca acgcggggtg atggcgagcg cggcgacgac
    26941 atcaccgcaa acgcccggac catccgggca atccccctga tcctgacggc cgacggcggc
    27001 ctgctccgca gggacatccc acggctgctc gaggttcgag gtgaggtata ttggccgctg
    27061 gacgatttca acgcgttcaa ccgcaagcgt caggaggccg gcgagccgac cttcgccaac
    27121 ccgcgcaatg ccacggccgg gacgctcaaa caactcgact ctcgcgtcgt cgcccagcgg
    27181 aaactcagct ttacggccca cggtttcgga gaggtcgacc cgctggagtg tgacacgcaa
    27241 tacgagcttt tccaggcgtt caaggcctgg ggcatcccga tcagtccgta catgcgccgg
    27301 gcgaagaacg tcgaagaggt gatcggtatc atccgggagt gggaccgtaa gcggggttcg
    27361 ctggactatg ccaccgacgg catggtggtc aaggtcgatc gactcgacca gcgcgagcga
    27421 ctgggcgcga ccagccgttc gccgcggtgg tgtatcgcct acaaatatgc agccgagcgg
    27481 gcccggacca agctgcattc cgtccgcttt caggtgggca agttggggac catcacgccg
    27541 gtggccaacc ttgagccggt gctcctggcg ggaacgacgg tcaagagcgc gagtctgcac
    27601 aactttgacc agatcgagcg gctgggcgtg cgcgtcggcg acatggtcta cgttgagaag
    27661 gccggcgaga tcatccccca ggtggtcggg gtggatatcg aggcccgacc gccggatgcg
    27721 agagaaatca ggccgccggc taggtgccct gagtgtgaca gcgagaccgt ccgggatgaa
    27781 ggcggcgtgt ttttgcgatg cgttaatccg gcgtgccccg cgcagatcaa ggaacgtctc
    27841 cgctatttct gcgcccgcga ccagatggac atcgagggcg tgggcgacgt gctggcgggg
    27901 caactcgtcg acagcggtct ggttcgcgag tttgccgatc tgtacaggct caaggattgc
    27961 cgcgaagagt tgatcgctct ggaacgaatg ggggccaaga gcgccgacaa tctgctggcc
    28021 gccatcgaga aaagcaaagc caatccgctt gctcggcttc tggcggcact caacattcag
    28081 cacgtcggcg tgagcacggc catcgatctt gccgagcatt tcggctcgat ggacgcgctg
    28141 attgccgcgt cgcgggaaga cctgcaacaa gtggaaggca tcgggcccga gctggccgag
    28201 agcatctacg gtttcttgca caatgagcaa ggccggcgga ccatcgccca tctgcgcgac
    28261 gtgggagtca atatgaccca gccgcgcaag gcgaaacccg gttcccagcc ctttgccgga
    28321 aaaaccatcg tcgtcactgg cacgctggag cactatggcc gcaaggacat cgaggatttg
    28381 attcgccaac tggggggcaa gcccgccgga tcggtcagca agaagaccga ttttgttgtc
    28441 gccggcgccg aggccggctc caagctcgac aaggcccgcg aactgggcgt cgaggtcatc
    28501 gacgagaccg aattccgccg ccgggcaggc gctgggtgac agcacaggtc gttcggacac
    28561 agcgataaat gacgtaagtg acttattaat aaagatttgc agatcactgt ggacagatga
    28621 agatacgtat cgtataatcc gatataagac gagaagtatg acgagaatga ttacaaaatc
    28681 gtttcgcatc gatgagaaga cggcccagga agttaatcgt tttgcttccg gactaaattt
    28741 ctcgcaggtc atgcaccaag ccatcgaggc ctggcttcgg gagcggcgac gggagcatag
    28801 ggaccagttg gtccgtcagt cctgtcgggc tcgcagcaag gccgaactgg cggaattcga
    28861 tgagattgca gagcaggccg gacagtcagc ccttgagctc atgaaggaag agtgagatgg
    28921 ccagaatctg ccggggagat gtcttcgagt ggcctaatgt ttcggacgat catcctatgg
    28981 gcagcaagcg ccgcaaatgg gtggtagtct ctcgcgagtc attcaacgaa gacagtcctc
    29041 acatcctggc ctgccccctc acaagctacc cccctcagcc aattgacatc agcgtcccga
    29101 agacaccgca caaccgactc gaccacgata gctcgctaat ggtctgcagg atcaccccga
    29161 tcaggaagac gaccatgggg cccgtcatcg cccatgtgga ccacagtgag cttcagcccc
    29221 tcatcgatcg cctggacatg atcgtgggcg attgaagagg ggcgaccgca tccctcatca
    29281 tttctctccc tgaccccaac aaccgcccga gtcgcgcgtc tgtttgagtg ttcggtttgt
    29341 tggcaatacc ccgcggacca tcgggagcgc ggtcatgctg cgcatcggca tcttctttgt
    29401 catcgtttta gtctccacgg acaagccggc cgtggccggc acccgggcac gcgagcgggg
    29461 tgaagtccct gccggggcgc gacctgcgcc ggccatcgac tcggtcatcc gattcgccct
    29521 ctcaagggat ggtttctcgt tcacggagac aggtgaaatc ctggtcaaga gtgctgaaaa
    29581 gcctgatatc gtccggctgc ccaacgacca tctgctcgtg gtgtttcgct ggcagccgca
    29641 gcccgaagcc gccgccgtcc ttgcggcggt gtggtcggcc gatgacgggc ggtcgtggtc
    29701 ggcacctcgc ccactggtgt tcaagggagg cggcggcgat ttcggcaaga tcgccggctc
    29761 gagcctcgca ctgatgcccg gcggtatggt gcggctctat ttcagtctgg cttcttcaca
    29821 ggacggggct gcccggctgg gatcggcagt cacgcgagat ggtttcacct atcagacaga
    29881 ccctgaagtc ggcgtgcgat gcgacggagc agatgtggtc aatccgctgg cgttctgggt
    29941 ggggcgcgaa ctgcatttgc tggcaacacc agtcaaaccg gcggcagcgc gggcctccat
    30001 gcgtccgccg cccgtttggg ttttcacctc ccccgacggc cgccatttca aggctgccag
    30061 accgatgcgt gagccggtga cacctggtga cgtgctgggc gaccgcgaag gctatcgcat
    30121 gtacgtctgc gaacgcggac agatccggtg catgggctcg cccgacggca tgcgctggcg
    30181 gcttcatccg gtgatatgtc tgcgaggagg cagtgacccg gcggtggtcc aactcaaaga
    30241 caacagctac ctgatgctgt actgcgcgcc gctcgatgaa cgttcggcgc ggcaacccga
    30301 gaaggcgctc gcatcggcgg gctcatcaga tgaggctgcg tccgatctgt tcgggcagac
    30361 cccgtctgga gctcccgcct gggaggcatt caccgaagcg gatacggccg ccgaggtggg
    30421 gtccgacttg tcgagtcctg ccgatttcac ggaggtttcg acgaccgacg ttccggatga
    30481 attcccgccg ctgccggact tccagaatcc ggtcaactac atgagctggt ataccgccaa
    30541 ttccgtgccc gccgccggcg aaaacgcgtg ggagtcttat gcggacattc tcctcggcaa
    30601 tcgtgaaggg cctgaatgga gggtgatcgg cgttctcgag cagggcggct ggaaaaccgc
    30661 ggaaccatgg gacccggccg ctcatcctga ttgggatgcg gcctttcagt ccgccgacga
    30721 gttgctcgtc aagttccgcg aggcgacggc ggaccccaga ccatacgggg taccccccct
    30781 ccgtatgacc gagaacggtg agtactatga agcgccggac ggccccggtt tgctcttcga
    30841 aaatctgctg ccgcatctgg ccgcgtcgcg atacctggcc aagacaacgc tggccaacgc
    30901 ctggcgcctg gagaacgggc agatcgacgc tgagaagatg aaggacgctt ggaccacggt
    30961 gctgggcaat gctcagcacc tcgagcaggg cgcctcgacc atcgaaaagc tggtcagcgt
    31021 cgccgagcgg gccctggtcc aggaacacgc caggtcggcc ctggagtacg gcgtattcca
    31081 aaccgccgat gatctcgagg ccgccctcga aaccctccag atgtacgatg tgcccgaccc
    31141 ccaccccgca agctacgtcc gcttcgagtg tgccgccagc ctggacgcga tccagtatgt
    31201 tttcgcgcca accggcgacg gttcggcgat cgcgtccgac cccgagaaga tcaaacagat
    31261 cggcggcaac ctgagcacga ccgaaatgat cttcgccgcg ctgctcccgg ccaagacggt
    31321 caacgaagct gtcgatctgt tgaactcaca ctaccgcgaa ttcgacgagc tgatggccag
    31381 gggatacccg gcgttccgcg aatcggactt cgccaaacgg gaacagcagc gagcgaagtc
    31441 caatcccatc accagaatca tcatgccttc tctggaccgc gtccgacaca tgcaaacccg
    31501 ggcggaggcc tcacgccggg caacccaact gtcctacgcc gcccacctgt tcaaggcccg
    31561 caacggccgc tggcccgcca gtctcgacga actgcccccc gagcaccgcg aatccgtccg
    31621 cgccgaccca ttcaccgacc aggatcttcg ataccgcctc gacggcgacg gcttcaccat
    31681 ctacactgcc ggcgaaaacg gcatcgacga cggcggaatc cacagcccca gcggcaacat
    31741 gaacaaagac ggcaattcgg acgactacgt cttctggccc ccgcagaaaa actgacgaca
    31801 gccatcccgt caacggatga ccgaggtttc tcagaccggg tggcatgccc acggctctgc
    31861 gtgggcatgt gtcaaccgaa tccgtcctgg acaacaaagt cccgatcacg acaattggtc
    31921 cggggcaaag gcccccacat gtgcggtctc ggaaaacaag cgaggttctt gtgaacgata
    31981 cgaagagaac gagatggttt ggatcggcat tggccgtgtt gtcatgcacg gtcagcctgg
    32041 gctgtgcggg gcgggccgat tctcggcttg acgcggtgat cgaccaggcc atccgcgaat
    32101 tcgaacccga ctggcagccg caacagggcg aaatcgacag gctcgtggtt ctcattgcac
    32161 cgatggcccg gcgggccggt tgcccagacg atacggaccg cctcaacctg ctcaccgccg
    32221 gctacttcta tcacctcgta cgggggggta gcggcatccc ggcgatgtac cgcaccgatg
    32281 ccgacctgct cccctccccg gacaccctcg accgcgccga cctcgcccgc accgcctccg
    32341 ccgccaagtg cgacctgtgc gtcatccttg agcctcccgc taagaacggg gcacaagccg
    32401 gtgccaagct gattgttcga gccgcttcat cggaaccccc gtcacagcaa tttgcggaga
    32461 ctgtcgcaaa ctcgctcggg gggcatgccg gccccgcgga catcgttgcc ggtctcaacg
    32521 cgcctgcgat cagaattcgg atggcggcga cgcaacccgc gacccggtgc ttcgccccgc
    32581 cgccccatcg cctcatggcc gagcgtcttt accgggccat cgcgactttc gcggccgggc
    32641 ggcgcgagtc gctggccgct tcgcgttcga cccgctggcc gcagagcgct ccgtcggccg
    32701 tcgatctggg cgcggtgcgg ccgatgcgtc ccgaagccga caggatcatg accatcgtcc
    32761 aaaccatccg gccgtcagga gatttacccc ttgagcaggc cgcgtggttc tgtgacatgt
    32821 ttcgccggac gagtctgacg gatgcaacca cggtctattt caatccgcag gtcagcatcg
    32881 aggaaggcga tgtcgtactg cgcggagcca cgacggcgcc ggctctggcc ggcacactgg
    32941 aaagagcact caagagagcc ggcattgaac gggtccgcaa cgagatgcgt tgcctgccgg
    33001 agaacgggcg gctcgacggg cacaggtttg ccgccgtgac cgtctcgacg gcgcggacct
    33061 acagcaggcc gtcggatctc ggtaatgttc agacccaact gctatacggt gaacttctct
    33121 ggctgctcga ccaccgcgac ggctggtacc ttgttcacgc gagcgacggc tattggggtt
    33181 gggtccgaca ggaggcggtt cggatcatcg agccgtcgca gttcgagtct ttgctcaact
    33241 cgaagcaagc ggcggtgctg aagaccatcg agatcagcgg aacgcaagtc ccggccgggg
    33301 cgcgcctgcc gctggtcgta caaacgccct gggacatctc catccggacc cccttgggtg
    33361 aagctgctgt cgttccatcc ggctcggtcc gcgtgatcga tgatttcgcg acgacccgac
    33421 cgcggatcat gcccgcgctg gaaatgctgt atgttccgta tgtgtttggg gcgcggtcgc
    33481 ctctggggct ggattgcagc ggtatggtca acaatctctt tgatcgcagc ggcctgccga
    33541 tcgcccgcga cgccacgcag cagttcctca gtggcaagct ggtggccacg cgctggtatc
    33601 gcgataccat ccggccgggt gaccggctct acttcctcga caattacggc aagattttcc
    33661 acacggggat tgcgatcagc tcgacgcatt tcgttcattc gtcgccgccg gccgtgcaga
    33721 tcagcagcct ccaaaagggc gaccgactct acgagcagta ttgggatcag tgcttcgttg
    33781 gggcgaagag gccgtgagga agcccccggc gatcagccgt cagcgatcag ctattagctc
    33841 cagccaagag gccattacag gtagacgaag tccgtcacac gcgggttttg atcgttgttg
    33901 cggctatgga tcgagccgcc ccggagatcg gctgccgcag gcaaatgcaa atcgttccga
    33961 ccggccttgc gggctcaagg ccaaggcacc ggacgatagg aagtcagcac cggatcaggg
    34021 cgagggctcg cggtcggcgg cgaggtacgc aggtcgatca cggtctccaa catggacgag
    34081 gaaaagaggc caattcgttg attgccggcc accacgacct cgttgttcat gtcgatgcca
    34141 ccgatcgcgc agccgatgta catcacgaga ccgttatttg gaaaatcctt gatgcgtatc
    34201 cgcggagcca agggccgtcc ggcaacccgg aaacagtggt cgataaactc ggtgcagtag
    34261 aaacggtcgt cgtcgggaat gaacttctga tcgtagagga catcctcggc gatgagcctg
    34321 tcaacctcgg ccagcacgag cgggacgaga tactgatact cgggccggag ccgccgcacg
    34381 accgtattcc acgtgccatc ttggtaccag tcgaccaggt atcgccgcga gatccccacg
    34441 ggcgagacgt cgaccaggat gatgccatcg ggagcaacgc gatagacgag cgcggcgtgg
    34501 gagaggtcgc tctcagtcgc ctccgcgacc accttgctga agtcaacaag accgccggcc
    34561 agcggggtgc gactgaggcg gaagatgatg tcgccctcct tcaagacagg ctcgagaacg
    34621 ggaacgtcct gtttggcctg ctcattgaat ttctcaacgg agaccgggat cggtgtcatc
    34681 tggcatcctg ctgccagaag cgcagacacc gtgagaaggc gttttgtcat atcgcaggtc
    34741 ctcgaaggca tcggccgcga agcccggccg gcgaaaggcc ggcggttcga agtcagcggg
    34801 aatcttaacg caaacgccca gaaaaagcca ttgccggggg taggtgcccc tccccggacc
    34861 ggccggggcc catacgcccg ctcgcaggat accataagca tcggcttcag acgcgccgac
    34921 ggtttagtac cacttgacct gcgccccgac aaagctcatt atccccttat cccgggcact
    34981 gttatcgtcg gcagcgggca gagcccgatc tgcgggattg cacagtcatc cgcacaccca
    35041 cgaaggaagt tgaccatgat ggcagccttc tcgcaggatg accccgacgc tgctgacaag
    35101 tttcgaagca tgtttggccc tgggcagatt gaccagcaaa tccgccaagc gatccacttt
    35161 tgctggatga cactgcccgc agacaaacgg aacgttgacg aggttgaaca ccagatgcgt
    35221 cggatcctcg atcgagcttt gaaggaccga cgtgacgatg ctcaggcgtt cgggattggt
    35281 gaagagtaag catgccggcc gagacccagg atctcgcgac cggcatgacg tcgggcaaaa
    35341 cccatgccac ctgccgcttg gcgcatgaac atgactgaat ccaagatgag gccgatgatc
    35401 ctgcgtgcgg ggatcgttgt cgctggttac tttggttcca cctttctcct gttcatcctt
    35461 gacagtgcaa tcactcaacc acacccggta tacgtgtctg gtacgtggct gacctacgcg
    35521 gcgcggaacg ctctcttttg ggccttgcgg tttggcgtcg gcgtggcggt tatcgtgaca
    35581 gttgacctgg gcgttcaacg aatccacggg gcttcatcaa aacgagacct gcgcttctat
    35641 ccgacagtca aaggattgcg aaatgccgtc cgcgccccga tgccccgctt ggtcatggtc
    35701 gcggggttat tgctactcgg cgggtactat gctctggttg agattgccat atgggagaca
    35761 agccggcgtc cacacgggct tatctggccc ccacatcgcc tgttcgtcgt gtgtctgatc
    35821 tcctggggtg tcctgtggat tgctgactgc ctgagacgac cgggtggcgg gacgattgtc
    35881 ggggcaattg ccttcacaac gtgggcgaca ctctctaccc tttcgattgc cttcggttgt
    35941 cttgtggagt agtcatgatc acccattcct cgctccgcga gaaatgggac aatctcgatc
    36001 aacagggaat tcgcaatgac agaatgcact acacgttcga agtgggcagc catccttatt
    36061 ctgtttctct cccccctcat cggctgcgtg acagatccgc aggctcgctg gcagggcccg
    36121 ttctttttca tccaaatggc cgacccgcag ttcgggatgt tctccggcga caaggacgtc
    36181 gagaaggaga cggctctctt cagccaggct ctcgatcatg ccaaccgctt acggccggcg
    36241 ttcgtggtca tctgcggcga tctgatcaac aggccgggcg acgaaggcca agccgccgag
    36301 ttcctgcgtg ttgccggccg actggacccc ggcattcccc tgcatctcgt ctcgggcaat
    36361 cacgacgtcg agaacacgcc cactcccgcc ggccttgaac gctaccggca acggttcggc
    36421 tcagactggt acgccttcaa ccacggcgga tgccgcttcg tggtcatcaa caccacactg
    36481 atccacgacc cgtccaaggc gccggaggaa atggagcggc aggaaacgtg gctccggcac
    36541 gagctgaccg acccgcaaca gccacggccg gtccaccgca tcgtcttcca gcaccatccg
    36601 tggttcaaca agacggccga cgaaccggac gcctatgaga acattcccct atcgcgtcga
    36661 ggccggtatc tcgattggtt gacgcaggcc ggcgtctcgg ccgtcttctg cggccatcgc
    36721 cacggcgaca gcatcacccg acacggccgg atggagatcg tctcaaccgg cccgatcggt
    36781 aaaccgctcc gaaaagaccc ctccggtttg cggatcgtcg aggtgtacag ggaccggctc
    36841 gtccaccggt actacccggt ggatgtcgtg ccggaaaagg tgtgcctggc ggcgaaatga
    36901 gggagccgtc agctttcacc ggtcggcaat cagcttgctc cataaggctg ttggccgccg
    36961 tgcctcgtga tgtagatgaa gacaaggagt agagagagcg gcctccttgc cgatggtttt
    37021 cttctcgcgc gacaagaacg tcaacaagaa ggcgcggcat gtactcgcgc acgcacgagc
    37081 aaagggttgt tcggtcttca catcggcaag ctttgctgtg ggccgtcaga ctccatcttc
    37141 cgcctcccct tcttcgtcgc tggaaggacg ggagacgggc cgaaagcaat ggcgcccgaa
    37201 ggccggacgg tggtcggtgg agtatctggt cctgaccgcg atgctgatga tgtgggagac
    37261 ggatgcggct ctgcgatgca gtctcatgaa ggcctgcgag aggatcgtcg agaggcttcc
    37321 caagcggcgg cgttgcgctt ttacgaaatg ggatgggaag cggaaatctg ttatcgcggc
    37381 tacaggcaaa cgctggaaca gccgaagctc aggagcgatc ccccggagca ggcccgccgg
    37441 gaggcgttct gggatatgac cgcaatgccg ctgttcggcc tgatgagccc ggaagccctc
    37501 ctgaaacgga aaccggaccg aaaaacctcg gcatcgcgac cgctcggcat aggattcgcc
    37561 gagccatgat tacgtccgcc tgctgccgcc gacggggcaa cctgcaaact ctcttggctt
    37621 tcgcggtcag ggacacatac cgtgggtacc atcctgactc atggccgacg gccgagagcg
    37681 agcagcttct tgtctactca tcaatcccta aatccgtctt ggtcatcaga gcctcgaaga
    37741 cgaaaccggc gccttcgatg ttctcacggg caccttcctg gcggtcgatc accgccacga
    37801 tcttctcgac ggtcgccccg gcctcggtga tggtcttggc tgcctcgagc acctggccgc
    37861 cggtggtggc gacatcctcg accaggagaa tgaggtcgcc tttctcgatc ttgccttcgt
    37921 acatcttctt cgtgccatag tccttcttgg cgttgcggat gatcacgaac ggcaagcctg
    37981 aggccatcga ggtggaggcg gccagagcca cgccgccgag ttctgcgccc gcgatccgtg
    38041 ttgtctgagg actgatatgt ccggcgaatc gctcgcccag ctcgcgaagg atcttcggtt
    38101 cggcctcgaa cagatacttg tcaaggtaat acttgctctt gcggcccgag cgaagggtga
    38161 aatcgcctcg cagcaaagcc gccgccgcca ctgcgtcaaa cagttcccgg tcggtcatgc
    38221 ctgtggtctc cataacagaa actcggcgag ggatcatacc acgaaggacc gcggcgcaaa
    38281 acaagccgcg acgcccatcg gccgttccgg acgtcccccg caggcgacgt ggaggctctc
    38341 gccctgggaa acgtgctccc tgttcgcaga atcgcgattg ccggcaggtt tcgctcgcca
    38401 acatgagata caaactgcgt ccgaatctca aaccggcccc cggtgttcag cccgcgcaat
    38461 ccggattgcc gggcacgccg gcacccgaca gacagctcaa aaagaccctc atgtccgcct
    38521 catcgacgaa ctcatcgccg tccagcgagg cgtcattgca gcgaggctcg gtctgcggca
    38581 cggtcgagcc gttgaggcac tcctgcaaat ggccgaaatc ctcctggtcc acgtcggtgt
    38641 cgaagtcgaa atcgcccggc acaggggccg acacgtggat ggtcgcctcg ggaaccgacg
    38701 agcacaagcc aaatacgttg ccgccgatgt tcgtgttctc cggcgtggtc tgcccgttat
    38761 tgaatccgag ccacacgacg gcaccgtcgc cgctgtcggc tattcccaga ccgcccacgc
    38821 agagaaacac cttgaccgtc cctggtgtca ctccggtcaa gctcagcgtg gcgaaccggc
    38881 tcgggttgtc cggcagggtg tcggtgttgg acagatcgaa ggcattggca tcatcgatgc
    38941 cctggccgcc cgtgtcggtt cgggttgtgc cggcagtggc cccgtaccag cggccgggtg
    39001 gttcatcgat cgatatcgac gcctgggctg accctccgtc gtcgctcctc agtcgcacat
    39061 cgaagctgat gccgtcgaaa cccagggggg ccgtggcctt gttgaaccag agatacagcg
    39121 gcaccgatct gccggccacc aactgcacat cggggttggt gacgctgccc aagcggctga
    39181 acgcggcttc ctcctgaagc cagaagaagg actgaccggt gctgatctga cttccgccgg
    39241 acgccgttgc ctcatgtccc ggtccctgcg gcaagccggc cccgtacatc cgatcggcaa
    39301 ataggggcac gtcgtcctga tcgaccagtc cgttgccgtt catgtcgccg gcgggactga
    39361 actgattgcc ggctccggcc atttgaacga aagagaaaat gtcacggttg ttggttcggt
    39421 tgtcgttgtt catgtcgccc agaccgtcga agccgttgat ctgcacgagg atgccggtga
    39481 tcgtcgtgac gccgggctca gctctggtcg gttcataggc cacgacgtca agtcgatggt
    39541 gcccctcgtt catcccggtg aacgtgcggg cgaacgtgtc cctgtctgtt tgcgtggcca
    39601 gcccctgccc ggcttcggcc agagcgacga cgtcggtccc cggctgctgg tccaggaaca
    39661 gatgtacccg cgtggcggtg cggtcgggcg aacgcaccgc aatctgatac atgctcgagg
    39721 tgatcggcgt acccggcggc ggatcggcca acgtcattgt cggagccagc cgatcgatca
    39781 gaatgacctt gcgccacgtc tcgaaaatcg gcggcgaccc ggcgggtcgg gaacggaagg
    39841 cgatgacgcc gagataatgc cgcccctcgg aaagcgatgt tgcatcgatg atctgacggt
    39901 agaggccctt ctgcacgccg ccgatgtcca cgccgcccga ttcgagcgag accgactcgg
    39961 tcaggaaatg ctcgtagcca taacccacaa aaccggggtc cagactgtcg atatagccat
    40021 tgccgttgat gtccattccc atgtccatcc gcagcagggc cagatcatcc tcggccgggt
    40081 cgagcggatc ggcgtcaacg gtctcgagac ggacctcgaa ctggtcggcg gtgatcacgt
    40141 caataggggt gagtcgccgc gtaccgttgg gtttggacgg cgagtccgcc gggatcgtct
    40201 cggccaccgg tgtcacgaag aggtcaccgt ctggattcac cggaccgtac actacgtaac
    40261 cgcgtccatg ccaatctccg ttcacgttcc tgtttctcgg cacgcggatc gtggcctggc
    40321 cgccggcgtc gaccgtgata acgtcccaga tggcgttggt gggatcgacg gctacgtcgg
    40381 tcgcattgcc ggtaagctcc ttgaggcggg tgccggccgt gaagctcgtc tgcaccgttc
    40441 tttgatcgta cccgttgtca tggcgatcgt tgaggcccac caggcaggca ttgtcacgtt
    40501 cgtagatcag tacgtctccg tcgatccatc gttcgaggta gctgccgcgg gcgtactcgt
    40561 tgtggacatc cacgagggtg gtgagatagc tgtcgtcgtt ccccagcgga tcggaccggc
    40621 tgggccgcgg aaagctgacg ctgccgaact caccggcctg gtagtagaca atcggcaagc
    40681 ccgctcgggt caggagatag gcataggcga gcagccccgg tgagggcagc ggctgcccgg
    40741 cgtcgtggtt ctcgacgaag gtgacaccga agtcgccgtt attggcgttg ccgtcgaagt
    40801 acccatcgaa gctggcgttc accacgctgg accagttgcc gaagccgttc tggctgagca
    40861 tgtcgtggat ggcgtaatag agcgggaagt ccagggcgtc gcgattgccg gtgaccccgc
    40921 cggaggtgtt gcagttgccg gtggtgcctt tgcacacgaa agcggcaatc tcgggattgc
    40981 ccaggatcga ctccccgaag cttagcggcg tggtcggcgc gccgctgaga tccggcttgc
    41041 cccgcagcca gacgtgacgg tcataaaaat cccgccaaaa ccagtcgggg gtatgtttac
    41101 aggcgtcgat ccgaaagcca tcgactccga cgacctcgat cagccatcgg cagtagcgca
    41161 gcagaagtcc ggtggcgttc tctgccacgg gatcgccggc catgggattg ctcgtgttga
    41221 acggatggat gccgatgctg ttggccggca gatcgttgtc cgaatagaac tgccggttgt
    41281 tcgggcttgg ccgatcatac gccgttccgg ccgggatgtt ctgcgggtcc ccggatgtga
    41341 ccggatgccg aatgtacgcg atattgaatt cctggccgat gtcggccagg ttcgagatcc
    41401 aacaagtggt cgggctggag ttgcaactgg ctccgggcgc atggaaatcg ccgtggcctt
    41461 cgggcccgcc gctgaccacg aatccggggt agtctccagt ggcctcgaac cccggcgtgt
    41521 ccttggacga gaagccgttg tggttcagga tcaggtcaat gaaggtgccg atggccgcct
    41581 tgttttgctc ggccacgacc gcctcgaagc ccgctcgcgt gccgtaacgt gtcgggccgg
    41641 acggggagcc gaggtcaaaa cggtcgaaga ccgcgtagcc gacgttcgag gttccacctt
    41701 ccgccttgct gggtggaggc gtccagacct ggccgtagcc ggcgatgaag gcatcagccg
    41761 tgcggtcttc tatggtttgc caggatgttt cgaaccactg gagagtaacc ggcacgcctt
    41821 ggccgacagc cgccgacaca agggccaagc atgccgaaca ggccatcgat ctcgtcgtcc
    41881 atcgtcttgc catgacccgg tgctccgcga cagacggcag ggtggagccg ccgtggccgg
    41941 gcggcgcatg tacccagccc cgggcgacag cagccgtcaa ttacccccgc gcccatcccg
    42001 gccaggacgt ggactcattg ctgggtcaac acctcgccca tgtagtttgc gtggtaatcc
    42061 tcacgcgccg ccgcggccgc ctctttccag aatgccggcc ctcggggcaa ggcggcttgg
    42121 agcccgtccc caaacccccg accggccgaa agcccgtccg gataggttct tacgagcggc
    42181 gtcgccgcag gcacaacagg ccgccgacca gcaccagaat caccgctgcc ggctcgggta
    42241 ccacatccac gcggatgatg ccggtcagag catcaacctg gaatacggcg gtcgggttcg
    42301 ctgcggtcac ttcaaaccag gtcgtgttcg cattgacgcc gcgaaagtcg tcgccaatgg
    42361 cgtcccaaga acccgtaacc acggccttcc actggtacca accaggggcc agcgtctgct
    42421 gatacttgta gatcccgccg ccctgtgcca ccatcgcggt cgccgggttg gcattattcc
    42481 agccctgcca gtcgccgacc gccgtccagg tccctggatc ggtgctcagg cccatgcggc
    42541 cccagttgcc tctccagccg tcctggatgt cgttggcgtt gaatgtgatg gtgaccttgc
    42601 cgtcaccatc gccaaagaac caactgttcc cgctgccggg ccagttttgg tcccaactcc
    42661 ccgtcgtgac cttgaactca tgccgaccgc cgacgccggt caggtttgcc tgccagatgc
    42721 cactgcccag gtcggtcatg gcggtgccgg ccgggttcca accattgaaa tcgccggcga
    42781 tgtaatagtc gccgaacaac ggagccacgg ccatggcgac cgcagccaca aagacaacag
    42841 tcacattcct catgttttcg ctcctcctct tatagagctg gctcaaatac tgaccctcat
    42901 accgcacaca cctagtacta ctgattactc cccaaagtcc cgcatacaag ccgatcaggg
    42961 ttccaccggg tcgtcgcaag tcttgtccgc tggaactccc ggaccggaag cacagaattc
    43021 aaacaggccg taatcctccg catcaatatc gaagtctccc tcccccgtcg cggggttcca
    43081 gtcaaagcat gcgcaaccgg ctccaacccc gccaccgctg ccggtataac aggcctggaa
    43141 cgcggcgaaa tccgcctggt cgacgtcatt gtccccgtcc gcgtcgtatc tcggatcggg
    43201 acagcctggc ggaggaggac cgatgtaagt ggtgaggcac gaatagcccg cggccgccag
    43261 atcatcaatt ccgtcgttat agttgggtcg gccgccgcct aatcctgccg ggaggaactg
    43321 atttgaaagc cacccggcgt aactgttggt tatcatcgca aacaggcaga ttggatcacc
    43381 ctcagtggga gaacccaccg catacgccgg gatcgagatt tcgaatccgg tggtggccgt
    43441 cttggcgtta ttgggctgga tacccaaggg gttatagatc gggtcatcgg ccgaccgatt
    43501 gttgacgccg accacgttca tgttgttgat cgcgacccgc caatctggta ccaccccgcc
    43561 gttgtcgagc acgccctcgc ccgagttcac cgggttggac ccgatgtaaa tgctctggtt
    43621 cgtctgaagg ttgatcagat caacgtaagc gacggaactc cagatattga ccaccagggc
    43681 gaaatcggct tcgaggggaa ccgtatcacc ggccattccg ccgattcgtc cggcgttggc
    43741 atccaaggtg aactcaccgc ccgccacggt gtcaaggaac acgaccaact ggttgccgtt
    43801 ggcctccaag ttgcctgtga cagcgacgtc gatggaaagg ttgtcgagcc cgtcctgaac
    43861 gaagatctgg ttgatctctg atccgggggc aagaacgggc tggcattggg cctgaacgcg
    43921 gtcaccgaag ctggtcaccg tgtcctggct ggcgacagcg ttgctcgtgc caaactcggc
    43981 cacaatgtcg cggccgtcca atgtggggaa gaaagcagcc ggcgtcagga tcgcctgggc
    44041 gaactgcgtt cccgtcaccg tcgtgaaatc gggccgcggc ccgagattcg tggcatggtc
    44101 attgccgttc tgaatccctg gcaacgtctg gttgctgacg tacccgtcgg gggcggtcag
    44161 aagcaccgcc accttgaggg ccaaatcgcc gctgatcggc ggcgtcagcc ccagatccgc
    44221 cagcgaaatc ttcgcctcca acccgcgcgt ttggcttctt gggtcgcccg gcaaaccgga
    44281 acccggcgcg ggcggggcgt ttgcgctctc gcccgtcaca ccggcggtgc cggtattgtt
    44341 gtaggcgtac tccgaaccgt tcgggttcgt gccgccggac agcatgccgc tgctgctgtt
    44401 gatctggact tcgcctcgga aaacgcgtgt cgcaaagtag tcgagttgtt cgatgggcgc
    44461 ctcggtacaa tcgcccaacc cgccttccgg cgtggttgga tcgttccact gcccgagcac
    44521 ggcggccgac agatcatact gtgtcacgtg cagccagccg ccgtctgtgt caatcgcgat
    44581 tgcgtagtca ggcgaaaaac ccgcttcgag cgtggtaccc gttgaacccg gatcacgaat
    44641 ggtctagttg ccgacgttcc gaatgagggg ctgaccaagt ccttgaacgg caccgggtgg
    44701 tccattaccg gggcaattct cgacaggccc atccggcgcg atctcggtgg ccagaacgtt
    44761 ctggccgccg tccgtcacgt ccagaaggat aatctgggaa ttgccgttct cctcgaggtt
    44821 gccggtcagc ccgatgtaca ggtaattgcc gtcggttcgc agataaagct cgtcgagttc
    44881 ggacccgttg gtcaaatagg tgcagactgc cgttcgatcg gcgaagctcg tcaccgtgtc
    44941 ctggcttgcg accaggttgg ccagaccgaa ttcactgacc gtgttcatcc cgtcgatgtt
    45001 cgggctgaag gcctggaccg atctcgtcac ggttgcatac tgattgccgg caacggcagt
    45061 gtagtcgggt cgatagccga ggttggtctg attggtgcca cgcgccgtgc ccggcagaga
    45121 ttggttgctg accaaccccc cgccgccgat gagaagtacg cttatcctga tcgtcaagtc
    45181 gctggtcagt ggtggtgtat agccgaggtc ggccagatga attttggcct ccaggccttg
    45241 ggtctgagtg cgaggatcgc ccggcaggcc cgacccggca gcggccaccg ctgtcgcggt
    45301 gacaccctgt gtcccctggt tgttgaaagc gtactgcgag ccgttggggt tggtgccgcc
    45361 gctcagcgtg ccgtcgttgc tgtccaccgc gacttggcct cgatagatcc gcgcggcata
    45421 gtagggcaga gactcaatgc ggtcgaagca gggagttacg ccgttgttcg ttccgggatc
    45481 atcccaggtt ccaagcgacg cggaatacag gtcatattgg gtcacgtgga ccgttccgcc
    45541 gaaagtgtcg accgcgatcg cgtggtccgg ttcgaagccg ctgtccagca acgttccggt
    45601 ggaggcggga tctcgaatgg tcaggtcgtc gctggggtca tccggcgtgc cattgttgtt
    45661 ggtggccagc gcctgcccca aattggggac cgcgaaagga ggcccgaccg aggaacatgg
    45721 catttcgctg atgatcggcg cgatctccgt ggcgaggacg ttctgcc
//
LOCUS       DAOTPW010000008        45232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_901_length_45232_cov_5.235452, whole genome shotgun sequence.
ACCESSION   DAOTPW010000008 DAOTPW010000000
VERSION     DAOTPW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 45232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45232
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_901_length_45232_cov_5.235452"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..768)
                     /locus_tag="P5316_01300"
     CDS             complement(211..768)
                     /locus_tag="P5316_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRR83619.1"
                     /translation="MGITFYHQCKPYELDRGVVFRDMQTRATGTGVLSVGTATFDPGR
                     YLPCHRHNCEEVIVILEGDAFVDVAGQRTPLKVYDSTHVPQGIPHRFVNASRTQHLTI
                     LWVYSVLDVERLVTDYTECMGAAPPEPGEDGYIAVRPRQLADLSHDELVARITTEVIA
                     ALRSHGMNKSEPLPGAPPHFDAEER"
     gene            complement(896..1879)
                     /locus_tag="P5316_01305"
     CDS             complement(896..1879)
                     /locus_tag="P5316_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR83620.1"
                     /translation="MTAFNENRYDSMIYNRCGRWGVKFPAVSLGAWQTYGGYVSDEVS
                     KKCIFRAFDLGITHLDFANNYGEPNGNAEIVCGKIIKEMPRHELLISTKAGYDMWPGP
                     YGEWLSRKYLLTSLDQSLKRLQTDYVDLFYAHRPDPNTPTDEVMDALDQAVRSGKAFY
                     VGLSNHGPDDIRAKNAHAVAYHTARPIISQSLYNMLDRAVERGVLRACAECGMGLIAF
                     CPLAQGALTDKYLRQNKPADSRLAQSSDICAKRLEQRDDIGKIARLNDLARRRGQTLA
                     QMALVWLLRRPEVVSVLIGASRPEQIEENVAALKNAKFSAEELAEIDSILA"
     gene            2463..3506
                     /locus_tag="P5316_01310"
     CDS             2463..3506
                     /locus_tag="P5316_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRR83621.1"
                     /translation="MIRFDDNPLITPADVKPSRPDFEVVCVFNAGVIRLGGEILLLLR
                     VAERPRPQPGELLAPMLCPDDPSKGLEVLRVRSGSPDVEEIDSRVFKYQGSTYLTSIS
                     HLRPARSKDGRNFTVADTPAVFPACREEEFGIEDPRITRIGDRFYVNYSAISRMGIST
                     GLAVTRDFVTYERMGIIFVPDNRDVTVFPEKIGGLYVCYHRPVSGMFGRADMWLATSP
                     DLLRWGDHKYVAGTRPGMWDCRRIGGGAVPFRTSKGWLEIYHGVDDAQRYCLGAMLAD
                     GERPERIIARSPAPVLSPEAPYEREGFFGNVVFTCGAVAEPDGRVIVYYGAADWCVAA
                     AETTIDELLASVR"
     gene            3651..4178
                     /locus_tag="P5316_01315"
     CDS             3651..4178
                     /locus_tag="P5316_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83622.1"
                     /translation="MTIELRCESCGKMLRATSERAGTRAKCPACGHELYIPMPPDQIE
                     ELPMTPEDAAELEREQALEEERRALDRALMRESISNDEPSARRGVRPPSRAGFGGGTG
                     GGNAREAVVAYLMAMRDANLDLAAQKLAVLRRQKAEALEVVDQLATDQLPPPSLANVP
                     PGVYQGFLRTLRSQL"
     gene            complement(4441..6384)
                     /locus_tag="P5316_01320"
     CDS             complement(4441..6384)
                     /locus_tag="P5316_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83623.1"
                     /translation="MQTRQPKRMINLIVCSLLLAIPGTILPAQAPAGEKPATSAKPVK
                     PAPPKDPTAVLKAIPASATAFVAVRNIAEVDYDVADILAKLELPLDQLGFPGLVAMIK
                     EQVGIDEGLELNSGAAVALLDCSDVKKVDEVHKRAVIFLPTDKPDALVKALGGTDKEG
                     DTYQLTLGSEPAIAAEKSGFLVIASAEAADALKEAVQAKEGIDKAMAPDRLKAYGDAD
                     LFAWAGMRRISKELRAEPINTLKGMMMAGTPGAKPEEIDDSIAEITKFLDEVKEASFS
                     LTLDSKTGLKMTFWYQATPDSDIAKRLSAVKPGDGSLLVGLPDEPMIAAGGWMNTLRD
                     EDVKKVVDQILKPEMVGTDVDEEQIKSIKESLVKLATSFEQAAVSVTSLPSESQDGLI
                     GATLVAKVANSQQAQGEIRKLFKTVKDTLVKTALGNGSLTEEDSKTIDEAIQLKENAE
                     KLTGAAVDHFVIDLAKIPDLEEEQLEQFKNVLGQEGIIIRIASVGERNVAITFGGGAK
                     RFAQVVDMVQKNQAPLADRKAIKMIADRLPATKRIGEGYLSVDQLLSTISDVFAKTGQ
                     MMPIPLNMSNAAPLSIMVTQVEPTAVEANVLVPIELAQSTAEMVKPMMMMFMGGMGGP
                     GMTMPEEEQEEEMEPTPTPPGVK"
     gene            complement(6653..7861)
                     /locus_tag="P5316_01325"
     CDS             complement(6653..7861)
                     /locus_tag="P5316_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR83624.1"
                     /translation="MSLTYATATCLKLAAIDAGIAFVVLLALAGIRRRKNRKTAVTIT
                     TTLLAGTVLVGACALKLVLLHRRGWDHFGLLNAVYTEMVFAVPLFSAGLLIAAFPWPR
                     SFLRATRPSVVVALLGLSAALVGVYASFIEPYRLIIETVTLPLPDDRDGLAPLRIGVI
                     SDFQTPQVTDHERTAVRMLMERRPDIILLAGDVCQANLAERERQIPAFRELHASMSAP
                     AGVYCVLGNLDYLSWVKRTMEGTGVRLLYNEIITVEFKDRRLLIAGLSGAYLGRAEEN
                     VIRRLQETGTPRDIRIILSHRPDAVLHLSPQSNVDLVVSGHTHGGQVRLPWVGALARS
                     SAIPRRAAGGGLFHINGHPLYVNRGIGMERGSAPQIRFLCPPTVSFINLIGPDKPSGL
                     PAGTALDEQG"
     gene            8008..10833
                     /locus_tag="P5316_01330"
     CDS             8008..10833
                     /locus_tag="P5316_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HRR83625.1"
                     /translation="MCCRGLRCCIPVVLAACGCAGHQASALPAFPGAEGFGAQTPGGR
                     GGRVIEVTTLDDSGPGSLREAVSAKGPRTIVFRLGGTIRLKSHLRVTEPFVTIAGQTA
                     PGGGILLRDAGLFIETHDVVVRFIRSRVGASLAEPYNTQDALQIGGMDARDIVVDHCS
                     FSWSIDECVGIRAPAHDVTLSWNIMSEGLRRPFSGEQIGEDRSHSMALILSGGPTRCS
                     LHHNLLALCNSRNPRIQGGRHAFFNNVVYGWGWLTGTFSRNPEVNFIGNYYKPGPGSQ
                     AIKAICERPGEMGRIYVKRNRSPDHPDDSSGEWESMVNGSAAEHRASEPFEMPPVTTT
                     SAEEAYAAVLRFAGAVLPTRDPVDARVVRNVRLSMGEKIDRPEQVGGYPEMASGTAPR
                     DSDHDGMPDDWETAQGFDPADRDDAIGDADGDGYTNLEEYLQFIVEEGLTPRPEVRGV
                     SIDRPGGPFVVECEAGHLPVVRVGGDERSFYTHAWFQDEVRLQVKPGAGGEVVSAQPS
                     RLRDRLKTSENGIELYVAEEGTRIFEQSPDGHRLFAFFDAYPGNAPELEGDNVIDAGR
                     FGVVGNRDNVTGKLQAALDAACKVRGGVVFVPSGDLEVDTIRIPSGVTLHLAVSSVVY
                     SSGRSTDGAMILFDGVEDARLTGPGVIDGRGDEGLPPVAAVAVRNARSIVLEDLLVRN
                     SSGLGIEVSDSRDVKINRSKILAVGNREGIGGLDVNRCESVTCERSFISSTGAGIRIR
                     SAGVGGAVGHTRGIRVREATVLSAGPGISVGPESDQVVGDVVVRDSDLFCSGEGISVV
                     AADGAGGIETAVFKDLTLDVRPLAGAPESGRPFLILNRSEGPMRNILFERIRAGAWAT
                     SRIEGLGSRPIEDVKFWGVEVTAKDIARSEPQPLFHVRHARCPQFRLLYVSWPAGGSA
                     GWSGVFRFDDVSDAVAPPAELFESTLR"
     gene            11426..11602
                     /locus_tag="P5316_01335"
     CDS             11426..11602
                     /locus_tag="P5316_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRR83626.1"
                     /translation="MKNLINRAKAFIKNEDGPTATEYAVMLALIIIAALTAITALGGR
                     VTEIFTEVTEGIGG"
     gene            11873..12427
                     /locus_tag="P5316_01340"
     CDS             11873..12427
                     /locus_tag="P5316_01340"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HRR83627.1"
                     /translation="MCYEYWLTACAIMVPGILWASWIDYKERRVPNWLNAAIACAGFA
                     AQGWFFGWQGLGWGVLGLAVGFSVLILPWLMHGMGAGDVKLMMAIGVWLGPALTLWSF
                     LVGALAGGVIAVIMIVSSGRLWNAWANIATIMHKLSNRSTIFSEFGSARSFGETSQLL
                     PYGVPLTIGTLGVFFAQLALMNAA"
     gene            12440..12880
                     /locus_tag="P5316_01345"
     CDS             12440..12880
                     /locus_tag="P5316_01345"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HRR83628.1"
                     /translation="MTRTHKGHRSRTLSRRFRRGAAVVEMAVVAPLLLTLVFGVMEFG
                     WSFMVHETITNTARECCRLATLQGSTDAEVYARFIEAMSGTGVNVTTDMITINRSPDV
                     DNPVVTISIAVPYSEVSITGLASFLGISTQNLRSTCSMRSEGTF"
     gene            13059..14156
                     /gene="cpaB"
                     /locus_tag="P5316_01350"
     CDS             13059..14156
                     /gene="cpaB"
                     /locus_tag="P5316_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HRR83629.1"
                     /translation="MMKSRAVIPLVVGLAVGGIAIKIFFNVLKKAKGSSVSDTVQVVR
                     AAADIASTVEISESMVGLVAVPKPLLPEGCFTDPKQVVGRVSSQTIPKGMPITASLLA
                     PEGTPPGMVAKIQDGYRAVAIQVDEVAGVGGFVKPGARVDVVMVVAGQGRNSNISRVI
                     LQNVEVLAVGQKTEVGAEGAAVTRSVTVLVKPEEASKLHLASMKGKLRLAMRSQEDRR
                     EPKPVTLTDQDLLTDGPPRNAKRNEDSLLARLFGNLPKTSKSPDDVRTGFKALPAPEN
                     KEWTVEVLSGTETFKVKFDSEGKSNHPVSTGRRKPNKDLSHSPSADVAAERVDEQPDA
                     VESGPVPESAAGGQGADVEGHASVFDKPSNE"
     gene            14193..15812
                     /locus_tag="P5316_01355"
     CDS             14193..15812
                     /locus_tag="P5316_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HRR83630.1"
                     /translation="MNGRFKDILTCDQPRPWLAVCAAAVLLCSTAGGQSTGTTDALIV
                     GQSAVEKASPLVLSNGQGRVQATVHRDDLAEERVTVTVGSSTVVDINVPVKRVEVASP
                     EIAGVTVLSPKQILVSAYKVGITQIILWSESEDRLLFAVEVEPNIAQIREAIAKLVPD
                     AKVELRVVNGSVVVSGRVSNVDDAARITDLCRISGAKVQNQMIVAGEQQVLLRCTVAE
                     VSKTAIRELGIDAWASFEANPAQTVREMIGLTDPSALLPSRNYVTGTSNFFGNRFLFG
                     STEQGAGFAISSKSLQMEMFVRALKRNGLMRILAEPNLVALTGQKAEFLAGGQFPVPV
                     PQRDNVTIEWKDYGIKLAFVPTVIGQQMIRLSVAPEVSELDYTNAVTLESYVVPGVSQ
                     RRTMTTIELASGSTIAIAGLLREQIRGEVNKFPALGELPVLGALFRSVNYQRDLTELI
                     ILVTPELVSGMMPDQVPGVPGKDVQDVSDWNLFGLGLLEGKPMPQDKEREGALLTEQA
                     PHVRKFASPPDQMSIHGPWGAADPSEAVVNR"
     gene            15936..18149
                     /locus_tag="P5316_01360"
     CDS             15936..18149
                     /locus_tag="P5316_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadG family pilus assembly protein"
                     /protein_id="HRR83631.1"
                     /translation="MCGSKRLDGAMRRRRHLRRGRRGAVAAQVAVCLVVLLGFTALTV
                     DVGYLYNARAELQRTADAAALAAVQELGGGKSDVLAMQSARQTAANYAAANTVLGQAL
                     VLEESDVVFGRAYLEEAGAKYTFEPGGTPANAVRVRARRTADSPNGGVPLFFARILGI
                     SQSDVSAQATAALTPRDVAVVLDLSASHNDDSSLRAVKKTEIHNYEVWSGLKDNNHAL
                     APKTDSLGFTSVVEVTDNGDGTSTVTVHLTSDGLEETAALSHVTFGLPEDAWSMAQST
                     ATSGGGYPVSTGVDPTTGVAGLKFDETSLGEDGAVETEDFSFKVPNDCLKYLMVVGTK
                     AGGEADTSAKYNLSPGPLLGNMNTWGDATAGPSWNPASDAGLVKLQKGANWSLSSAFV
                     SQTLKMRGYGQYNAAEMSVINSSSGDSTTAAYKRRVRVALGLDRWKSGKPGGQPGGNG
                     DNVIDAGEVVPMVAYPSQNVNPDSYSKKVGGTWDSYIDYVISSSSGMCVYDPAKEYYG
                     DPNLRYSFGLKTWVDYLQECQVGEAASPGMAGVPTQPMGAVADAVKECVNIIAGLEGD
                     DLVGMASYGTVSYGPADKPNNMSWLVDDFPSLLAKVDTLQAGMWTSYTNIAQGIDEGV
                     EVLFNSPNGRRNAAKIMLLLTDGVANQTRNPVSYNTYQAREDTKAAARDARDRGVRIY
                     TVSVGVSADKDLMAEVASIGGGSWFHAEGDIESYKDQLKAIFRELGGKRPGVLID"
     gene            18357..19550
                     /locus_tag="P5316_01365"
     CDS             18357..19550
                     /locus_tag="P5316_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR83632.1"
                     /translation="MRQSVRVIVFNADEEYSAALRADLLSIPGVQIVAEVDEATLIEQ
                     AVTHFPAEVVVIHLDPMPEALLPVASSVASNHPEIAVFVVTSSTDGQYVLAAMRAGVK
                     EFITKPVDRQLVATALDKVLAQNESAHERGVLISVMGTIGGSGASTVATNLAVELAEF
                     AKKKPVAIVDLDFRFGQLSTMLDLQPDYTIADLCDTPEKIDAGVIEKAMVRHNTGIHV
                     LARPNHFSEADQITAAHCVAVLSSLQQMYEYVVVDGPSRYDPGGLAVLDMADMNLLIM
                     QLLVTSVRNVHRMLDELRAGGYNMDRFHLICNRVERDSNNLEISHVEETLKKDVAHQL
                     PDDWKTVSTAINMGVSLKEYSAKSRIRLALRELARRIAQPENADEAHENGGKGGLFSR
                     LLNAV"
     gene            19604..21091
                     /locus_tag="P5316_01370"
     CDS             19604..21091
                     /locus_tag="P5316_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRR83633.1"
                     /translation="MFGKVRTPATPKIPPRTEKPEGGKTILGPRPPLSGTQPGGGVAV
                     EDEPKPAPAVQPDKAKVRQADDDKLEKYYALKTRIHRKLVEQLDLNAIDNDDPTIRDQ
                     VGEVILSLCEKENVLLNHNERQQLVKEILDETFGLGPLEVLLKDSSISDILINGPRQI
                     YVERKGALELTDVQFRDNEHLLHIIDKIVSAVGRRCDEVCPMVDARLKDGSRVNAIIP
                     PLALDGPSVSIRRFGNDPITWDDYIRFKSCAPEMVEFLKACVIAHLNIVIIGGTGSGK
                     TTLLNNMSSFIPPTDRIVTIEDAAELRLRQPHVVRLETRPANIEGKGRISIRDLLINA
                     LRMRPDRIVVGECRGAETLDMLQAMNTGHDGSLTTLHANSTRDTMQRVETMVMMAGFD
                     LPVRAIRQQFASAVDLIVQAQRLTGGPRKIVSVTEVVGMEGDVVTMQDIFQFQQLGID
                     EQGKAYGQIVSTGIRPSFLDRLKYAGCDVSPSLFERQVLVKDKDE"
     gene            21156..22139
                     /locus_tag="P5316_01375"
     CDS             21156..22139
                     /locus_tag="P5316_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRR83634.1"
                     /translation="MESLSALLFLIMPLAGATLLAYGIFQVVMDLRGGEQRKMLDRLS
                     GAKSVDRELKVRESLLRKTAAETQGNFLESLVAKLHLVKKLQSVLDQADLGWSASKVL
                     VNLAGAALAVVLVMVLLQISPLVILLVSVAIFGLPILYMIWQRKRRIRKLVEQLPEVF
                     DLMCQALKAGHSLASAIQLISQQMPDPVAGEFARVFQEQNLGLTVEDALLNMTKRVDQ
                     MDVRFFVTAVLIQRQTGGDLAEVLEKIGKVIRDRIQLFGVVRALTAEGRLSGWVLLLL
                     PVLVFFGMMVVNPGYAAELTDTDSGRKMLGIAIGMDLMGMAMIKKIVNIKV"
     gene            22144..23106
                     /locus_tag="P5316_01380"
     CDS             22144..23106
                     /locus_tag="P5316_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRR83635.1"
                     /translation="MSEMVLFGILIVASVGLIVYSLWPAKKAESDHIRRRMAGRRLDK
                     AEALLTVAPKRERSAAKEMIEKVAPIAMRPVMPASEEEMNKLRSRLSQAGYRSEAALR
                     YFLASKTIMGGGVLLVALLFAMGMGLETRQIFGIAAFAGGIGFMLPNFWLSLAQSSRQ
                     ERIRNGLPDVLDLMVVSVESGLGLDAAIMKVGEDMHNVHPELAEEMMIAVAEGQMGLP
                     RMEALEKMAARCGVDEVRALVSTVAQAEKFGTSIAKALRTQADSLRVKRRLRAEERAQ
                     KTTVKLMAPLILFIFPSIFVVLAGPAAMKLVAAFSRGGISGAPH"
     gene            complement(23236..24105)
                     /gene="dapA"
                     /locus_tag="P5316_01385"
     CDS             complement(23236..24105)
                     /gene="dapA"
                     /locus_tag="P5316_01385"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRR83636.1"
                     /translation="MFKGSMVALVTPYANGEVDWKTLDALVDFHLDSGTEVLVPCGTT
                     GESPTLTHEENDAVIAAVVRRAKGRVAVLAGTGSNSTAEALVMTRNALKNGADGALLV
                     VPYYNRPTQEGLFQHFSTLAKAADFPQVLYNIPGRCGTELSVDTIVRLRETHKNIVAV
                     KHATGSMDGASELRVRSDIDIISGDDSMTLPLMSIGGVGVISVLANIVPKDVKALTDA
                     ALRGDWETARKWHLKMFKLAKGLLTLEVNPIPIKTAMAIKGMLTEEFRLPLCPMSPAN
                     RERLLAVMKEYGL"
     gene            24379..25767
                     /locus_tag="P5316_01390"
     CDS             24379..25767
                     /locus_tag="P5316_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83637.1"
                     /translation="MEKPSSERKPEVNRRDFLKGAAASAGAAMGVVVLEGCAGLTRVG
                     PAAAIPAFKPRDVVRIGFVGVGGMGTNHCNNLLKIEGAELRAVCDIVPEKVAHVQRLA
                     EQAGKPKPVGYSAGPWDFKRMCDREDLDLVYTATPWEWHVPVCLYAMKTGKHAATEVP
                     AAMTLEECWQLVETSEQTGRHAIMMENCCYDRSEMMVLNMVRKGLFGEIVHAAGGYLH
                     DLRALKFSSAGEGLWRRAHGWRLDGNLYPTHGLGPLAWYTNINRGDRFDFLVSMSSAA
                     RGMQAYAAEHFPPDDPRRKEKSVLGDVNTTLMRTVNGVSIMLQHDTNLPRPYSRINLV
                     QGTKGIFRGFPDQIYVEGRSKEHEWESPEAYRKEFAHPLWVTMEEKAKGAGHGGMDFI
                     EDYRLIKALRHGWYPDLDVYDAATWSAVVDLSVKSVAGRSRPVDFPDFTRGKWKTTPA
                     IPIFDADQPCRA"
     gene            complement(25733..27313)
                     /locus_tag="P5316_01395"
     CDS             complement(25733..27313)
                     /locus_tag="P5316_01395"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRR83638.1"
                     /translation="MNDSSLLLLAPEVTLVAGASLVLLIGLARQEAVRASSFLLAFLT
                     ALAALAIAWAAGPLERPIRAFGVELTSLTWYVRLTALGIGLVVLLVHHHIPQEEQRGE
                     IFSMILFSLTGIMLTTMADDLVLLFLAVELVSVPTYILVSMGRSDIRAQEAGFKYFFL
                     GALAAALLAYGLSFLYGASGDTCLSRMNLDGNHAYVIIGMLLAFSGIAFKIAAVPFHA
                     YAADVYQGSASPTAGLLGFFPKLAGFVALIKLLMLVQPRGTGPLGWEIPDIGFAFLWI
                     AAVATMIVGNALALLQTNVKRILAYSSVAHSGYMLVAVLAGPVAGGGPLRNGLSAMLF
                     YVTVYGLMNLGAFAVLSVTRADGREVEELDDLAGLSRCHPSAAFALAVCVFSLMGLPP
                     TAGFLGKLYIFSSALSVDPSHPHRLALIALAVIGVINAAIAAAYYLRIVAACYLRPPR
                     YRFALVTRSRPLQFGVFCCCLAVLLIGLWPAPLIRMSSQPFLELRPPSMLAKNSPFPQ
                     PPSRRQAIKHDTAGPHRR"
     gene            complement(27306..29078)
                     /locus_tag="P5316_01400"
     CDS             complement(27306..29078)
                     /locus_tag="P5316_01400"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRR83639.1"
                     /translation="MTELAETWILTLLITLPLVGAVVVGLAEAREADEAAARRIRRTA
                     MGFSLATAILTLLAILLFFGAVDRSGRSLAEQGRYALCLDRLWVSDEETAPSAARGAG
                     YVDFRYHVGVDGISLWLVVLTAGIAPLTLWSGFASIRRRVKEYHVLMLLLEAGMIGVF
                     CARDLLLFYIFFEFTLIPLYFIIGIWGGPKRQQAAHKFFIYTFAGSVLTFAGVLFLAY
                     QAYSLPASANGVGVFTFDLETLYALRLPPETQWWLFVAFAAGFAIKVPLFPLHTWLPL
                     AHTEAPTAGSVLLAAILLKLGTYGFLRFSLPMLPAATFAFAPLMAILAIGGIIWGALA
                     AWVQQDIKKLIAYSSVSHLGFCILGMFSLKSAGLVGSVMYMLNHGLSTGALFLVVGMI
                     YERYHTRRFDQLGGLARPMPWMAFFLVFFTLSSIGLPGLNGFVAEFLVLLGTLTSNAD
                     GTGPLGIMHAAFAASGVILGATYMLYLCRRVLFGPLREPARAPEDYAGLSRDLDKREI
                     GILAPIAAACLVLGLFPNVILSSIRPAADRQILARLRETPGKTQDSGITVQRHELPDE
                     TFLNRRSPLGTTRRASWHGGLDYE"
     gene            complement(29086..31077)
                     /gene="nuoL"
                     /locus_tag="P5316_01405"
     CDS             complement(29086..31077)
                     /gene="nuoL"
                     /locus_tag="P5316_01405"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRR83640.1"
                     /translation="MMLAAVDTDILNLAPLVPLLPLLGTAIAGLAGPRAIKGASHWPI
                     IIGVGAALVCSILLLLEVASLPVRAYDDPYIFYSWFVPAAGVSFNISFQVDPLTTIML
                     TTICGVALLVVIYSRDYMRHHGRPQRGYERFFAFMGLFVCSMCTLVLAGNFLLLYLGW
                     EAVGLCSYLLIGFYYQRPAAAGAAMKAFVVNRIGDFGFGLAVLLLYRWLAPHVPPGES
                     PLDYATAFSYLGHLTAGQTTTIALLLFCGAVGKSAQLPLHVWLPDAMEGPSPVSALIH
                     AATMVTAGVYMVVRCGPVFAASPVALNTVAVVGAATALFAATIAIAQYDMKRILAYST
                     ISQLGYMFLGVGVFAADSAIFHLFTHAFFKALLFLGAGSVMHAMGGIIDIREFRGLKS
                     IMPVTFLTFLIGSLALAGFPLLSGFFSKDEIIHAAFVFHPVYGILGMTTAILTALYTF
                     RMMFIAFHGEPRIPEGVHPHETGRWMLVPLVLLAAGTVTAGYAGVAIRPGGFAGLFEP
                     SGAFHEFLAPVVAPFKEASIPRLGHRTHGDGYALMYVSAALAILGIAAAYVLFVRRPT
                     WSQTVRQAFPRIHGLLVNKYYVDEAYDALIVRPLWRLGASLYALDQFFIDGTLRLIVA
                     VPQALGAALRTLQTGATQGYGVVMTGGIVLMALIVWLMK"
     gene            complement(31151..31453)
                     /gene="nuoK"
                     /locus_tag="P5316_01410"
     CDS             complement(31151..31453)
                     /gene="nuoK"
                     /locus_tag="P5316_01410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRR83641.1"
                     /translation="MDLTNAYILVGAILFGIGVAGFLARRNMILMFLSTEVMFQGVIL
                     NLVGFGLKHGHLTGQVFSLFLVVVAAVEASLALGLVVLLFRRRTTLDSHAWEEMRG"
     gene            complement(31618..32463)
                     /locus_tag="P5316_01415"
     CDS             complement(31618..32463)
                     /locus_tag="P5316_01415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRR83642.1"
                     /translation="MTTTGMYILYAFLALGAAGVYLSMPAGRSTGRPLRWTARILGAA
                     SLAAIATYCVRWIGEAFDGRAFFVLFALVAIVAAVRVVTHPKPIYCALYFVLVVLAVA
                     ALCLLALAEFLGAALVIVYAGAILVTYVFVIMLTQQQETDPANLTGREPLAAVTLGFL
                     LSAAVVQAMVSADSASQMANTGVLVGATESEGRSLAASTNAGQSSPVDDESAGNVRLI
                     GKSMMTTYVLAVETAGILLLVALVGAVSLARKRIEAEALTPAELAAQAEETDIKRRGR
                     EAPPF"
     gene            complement(32460..33053)
                     /locus_tag="P5316_01420"
     CDS             complement(32460..33053)
                     /locus_tag="P5316_01420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRR83643.1"
                     /translation="MIKDEDIIEITEPKLTAADKLYLPQIINGLKTTFRHLFAKRLTV
                     QYPEERVKVRKEIYRGVHRLNRDEQGRVACVACFMCETACPAHCIHIVGAEAPWPDRE
                     KYPVRFDIDELVCIYCGMCEEACPVDAIELTPEYSIVGYSRQEMIFDINKLLEVFDQT
                     RDEKPRKNPPITGYGGTGGLISPSPRPYGNETEAGSL"
     gene            complement(33068..34408)
                     /locus_tag="P5316_01425"
     CDS             complement(33068..34408)
                     /locus_tag="P5316_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRR83644.1"
                     /translation="MNVLTLILAAATLAAPLPEADILGQADARIEKHRKGDIVLRLTN
                     NGRVPPAGLHVRIRQTRHAFLFGSNIFKLGACGSPEANAAYEQHYAALLNYATLPFYW
                     WHYEKDRGRPDYDKTKVMADWCAAHNVMTKGHPLAWNWFDPKWLPDDPKEVLRLQLER
                     IEECSRRFAGQIDIWDVVNEATHYDRPDCKKNSPRLTKIIRDMGVGPFVRAAFQAARK
                     GNPKATLIINDYAVGDEFAAKVIDELVDDEGRPLYDVIGIQSHQHGGAWPAEKIWDVC
                     ERFARYGKPLHFTETTFISGAQGRNLAEQRQQAGSKFKWASTPEGEKRQAEEVVRFYT
                     ILFSHPAVEAITWWDFTDYNAWQGAPAGLLREDMTPKPAYEELMKRIKGTWWTTETVA
                     TVEGNGEVRFRGFLGDYTVTTADLGGFSDRPLSGDFSIGKTNQKPIEVDLAFIP"
     gene            complement(34405..35613)
                     /gene="nuoH"
                     /locus_tag="P5316_01430"
     CDS             complement(34405..35613)
                     /gene="nuoH"
                     /locus_tag="P5316_01430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRR83645.1"
                     /translation="MIDLLKTQLGFSVALMVVLITIIMGFVAYAILLERKVCAWLQDR
                     IGPNRAGPLGLLQPIADGVKFLLKEDFTPRGADRPLFFLAPCIAFAVAMIGFAVIPWG
                     GPLDLGGPEPVRVQVAGVDIGLLYILAVGAMGVYGLVLAGWSSNNKFSFYGGMRAAAQ
                     TLSYEIPMGLAILVVVLTTGQVRLEKIVEYQMQSTWNVLLHPLAFLILLVTAFAETNR
                     MPFDLAESEQELVGGYHTEYSSMKMALFFLGEYAHLITVSAFMTALFLGGWELFPFST
                     RLGWGWLDWIYKSQTLAAALCQFAIVLSKVVVFILFFMWVRWTLFRPRFDQLMRFAWN
                     GLVPAGIALVVLTSLLLYKNVVASFWSPLCNAAVFGAAVLRFYVWSPAITGRQANLPP
                     IEGSLTRRFE"
     gene            complement(35616..37241)
                     /locus_tag="P5316_01435"
     CDS             complement(35616..37241)
                     /locus_tag="P5316_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRR83646.1"
                     /translation="MPTIIIDGRTIQCRDGITVLQAALDAGIDVPHYCYHPGLSVVAS
                     CRLCLMEMKILDPKSGDLEWSPRLIPSCQTPVRDGLEVRFETEKIHEHRKRIMEYYLL
                     NHPLDCPVCDQAGECYLQDYSLKFGEAVSRMVDPKTVNPKKDVGPRTLLYQDRCVLCS
                     RCVRFTREVSGTSELCIVNRGHVAEIDAFPGRPLTNKLQGNVVDICPVGALLDKDFLF
                     AQRVWFLTTTNSICRACSTGCAIRIDQNRNRVYRLRPRWNVGVNDWWICDDGRFGWKF
                     ILDEKRLSTPAVRCENGHEPLLWESAPELVRRRLSQTIDRHGPASVAVQLSPEMCCEE
                     AWLLADFVRRIAPHAALSLGDVHTIGEPEVFPVGAAEESARFVIRPEKHPNRRGIEAI
                     LTAMGGPVLPREELWKEAAAGRFQALWIVGGYPDNDWPTQTLPDAAAKPELLIVQDMF
                     PNRLTARADVVLPFCAWTERQGTFMNHAGLVQPFERAVDPPEGAMHDGQYLYAIAGHT
                     GLYASEKVRKMMIPTVPAMADVHVPEPQPRFQH"
     gene            complement(37373..38722)
                     /gene="nuoF"
                     /locus_tag="P5316_01440"
     CDS             complement(37373..38722)
                     /gene="nuoF"
                     /locus_tag="P5316_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRR83647.1"
                     /translation="MKRFEPVLLRNVGVAGVRRLETYRARGGYAAAAKALRTMTPQQV
                     VDEVKRSNLRGRGGAGFPTGVKWSFLPADRSITYLCVNFDESEPGTFNNRYIVDHDPH
                     QLLEAILIAGYATRATVAYIYIRVEFHETFHILQKALDEAYAAGLFGKRILGCDYSLD
                     CHIHRGAGAYVCGEETGLIESLEGKRGWPRIKPPFPAIKGLFGQPTVVNNVETLCCLP
                     HIIEQGAAWFRSIGTPGSNGPKLYTISGPVNRPGCFEAPLGLTVRELIYGDDFGRGMI
                     GDKKVKGVIPGGLSVGILTGDELDCKLDFEDPVKYGLLGLGTAGAIVINEETDIRKVL
                     LNVTRFYAHESCGQCTQCREGSAWMYKIAKRIAAGAGRSEDLDILIENSHNMGMIPGM
                     SICGLPDGAVYPIRTIVEKYRSEFEEHIRRQEPAAVAQTLRRINPAVYEMPIAGRKG"
     gene            complement(38815..39387)
                     /locus_tag="P5316_01445"
     CDS             complement(38815..39387)
                     /locus_tag="P5316_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRR83648.1"
                     /translation="MAWRTIDRNQPAPDDAESPQISEAVKARIRGFFTRYPSKRAALL
                     PALHIVQETYGCVSRRAMRDLAELLELAPSEVMDTASFYTHYWTKPRGRKVIVACRGL
                     SCELMGVQEVNRAVCEHLGIREHETTADGEYSFVTEECLGACEYAPCLLVNERLHKNV
                     RPEDVPRILADPDNDKIGLERSDLYDGVKA"
     gene            complement(39679..40911)
                     /gene="nuoD"
                     /locus_tag="P5316_01450"
     CDS             complement(39679..40911)
                     /gene="nuoD"
                     /locus_tag="P5316_01450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRR83649.1"
                     /translation="MRQGLLEGPGHRELWCLNFGPQHPATHTTLRLVLELDGETVVAC
                     TPHIGYLHSGFEKLGEHLDYNQYVTITDRMNYISPMANNIAWHHTCEKLFGIEITPRC
                     KAIRTIIAELARIQDHLLCVGAAALDLGAFTAFLYGFNEREWINDLFEEVCGARFTTS
                     YTRVGGLMFDIPPEWPRHVKEFLARLPRAISDLENLLNRNRLFIERTKGIGYMSRDDA
                     ISWGWTGPLARASGVRRDLRKDEPYLCYADNWDGKGSSGVSFQVPIATGGDVLARYLV
                     RIEEIKQAAGIIHQLIDNIPPGPVNVLPNDKVTLPDKREVYFSIEGLIHHFELIMTNR
                     GFTPPVGEVYGANETANGELGFYLVSDGGRCAYRARCRPPSLVHFQAFEHLIVGHQIS
                     DVVAVLGSLNIIAAELDR"
     gene            41209..42174
                     /locus_tag="P5316_01455"
     CDS             41209..42174
                     /locus_tag="P5316_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83650.1"
                     /translation="MKSSLQLVPMHVGQCTLGKNHVLGDPHSDEDRVDFTLYAFLADG
                     GPGRRALIDLGPVGLPYINWMFRRYGFFRDLPGAPDDVRQPHGNIFDWLSRLNIAPES
                     VGHIVFTHLHADHHGLTDGKDGGAILRFPNARIYVSRTGWEDNLTKRVNGGWNSYVDF
                     AFSDFLLDADKAGRLAVLDEGEVMPGVTVSYLGGHSVCSLAVRIETPSGAAVVCSDDV
                     YRYDLLEQGILARLRVTHARLLAATERLVGMAEAGAILLPCHDPGIFDMYSRYKQEWL
                     GHLKPVSHRAAAGFRRSAKVLLGRLPDDSVPSPARPRDCKTPAMP"
     gene            complement(42471..43415)
                     /locus_tag="P5316_01460"
     CDS             complement(42471..43415)
                     /locus_tag="P5316_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRR83651.1"
                     /translation="MIAWCRRNAGSIADQAAAVLAVYWVVALLIRLTVRDSFVPASTI
                     FYATPIIVLAGIAAIACLLLLVRRRRRMGWLCLGAAVACMAWWHCATRFTSATASDGK
                     GVRVLAWNTAGRALERPGVIDQIRRVNPDIIAMNEAGPATKERIAQYAAAFPGYNIST
                     IYHGMILITRGELVEHECGDLADRAWYKHTSVLVGGVRLSVIQPEIRSNPFYSRAAAI
                     QTLTRLACSMSNEPTIIIGDFNTPADSIHLRGLRQDFLNAFEAAGKGHYATWPIPCPV
                     LAIDQAWVSRLLRVTRCELGWTWKSDHRPLICEIQLAN"
     gene            complement(43526..44572)
                     /locus_tag="P5316_01465"
     CDS             complement(43526..44572)
                     /locus_tag="P5316_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRR83652.1"
                     /translation="MAKKQTKRYRFSFGPWNIHEGADPFGPPVRQPVSFAKKLKVYKD
                     LGFDGVQFHDDDAVPELDTLPASTVLKRAAQLGKILKDHGLVPEFVAPRLWENPMTID
                     GGYTSNSAACRRYAIDRSLQAIDIAFELGANQIVLWLAREGTYIRESKNARIAHEYLM
                     QAINKMLNHNKEVRILIEPKPNEPMDHAYVPTIGHAISLAYLSDDPSRVGGLIETAHA
                     LLAGLDPSDEMAFALYHKKLWSVHLNDQNGLKFDEDKSFAADNLRRAFNQVRVLEEGG
                     FGLNGEMVGLDVKVMRTQKQSNNTAHLANSKRFFELLLAKVRTFNRKKEAEFIRNRDY
                     EGLERMVIEHLLGR"
BASE COUNT         8551 a        12938 c        15111 g         8632 t
ORIGIN      
        1 cggcaggtga aacctgcggg ttgccgcgca agccggcccg cgttgcgggc cgcgtcacgt
       61 caagggtgag accccgggta taggtgtggg tagtcgcgtt cgaagcagaa aacggggcgg
      121 gtttgcttcc tggcgatccg gaacctgacg gcaaactgag acggacttgc gaaacgccca
      181 ggggctgctc ggatgtgccg gggggaccga tcatcgctcc tccgcgtcaa aatgaggtgg
      241 ggcgcccggt agtggttctg acttgttcat cccgtgggat cgcagggcgg cgatcacctc
      301 agttgtgatc cgagcgacca gttcgtcgtg cgacagatcg gccagttgcc ggggtcgcac
      361 ggcgatgtag ccgtcctcgc ccggttcggg aggagcggct cccatgcatt ccgtgtaatc
      421 ggtgaccaac cgttccacgt caagcacgct atagacccag aggatggtca ggtgttgcgt
      481 tctggaggcg ttgacaaagc gatgagggat gccttggggc acgtgggtgc tgtcgtacac
      541 tttcaggggc gtgcgttgcc cggcgacatc cacgaacgcg tcgccctcca ggattacgat
      601 gacctcctcg caattgtgcc gatggcacgg caggtaccga ccgggatcga aggtggccgt
      661 cccgacgctc aggacgccgg tgcccgtcgc cctggtctgc atgtcacgaa acacgacgcc
      721 acggtccagt tcatagggct tgcattggtg gtagaaggtt atgcccatac cctgcttcct
      781 tcagaagaat gagcctcgac ggggattata cctcatcggg ttcgcccatg cacagggtac
      841 ttcgtttacg cgtttcttgt ggttcccgga gggttcgtgg cgagaccggc gcgatttacg
      901 ccaggatcga atcgatctcc gccagttcct cggctgagaa cttggcgttt ttgagggcgg
      961 cgacgttttc ctcgatttgc tccgggcggc tggcgccgat caggacggac accacttcgg
     1021 ggcggcgcag aagccagacg agggccatct gggcaagggt ctggccacgg cggcgagcga
     1081 ggtcgttgag cctggcgatc ttgccgatgt catcgcgttg ttccagccgt ttagcgcaga
     1141 tgtcgctgct ttgggccagc cttgaatcgg cgggtttgtt ctgcctcagg tacttgtcgg
     1201 tgagtgcgcc ctgggcgagg gggcagaagg cgatcagtcc catgccgcat tcggcgcagg
     1261 cccgcagcac tccgcgttcg accgcgcggt cgagcatgtt atagaggctc tgagagatga
     1321 tcggtcgagc ggtgtggtag gcgacggcat gggcgttttt ggcgcggatg tcgtcggggc
     1381 cgtggttgct gaggccgaca tagaaggcct tgccggagcg gacggcctgg tcgagggcgt
     1441 ccatcacttc atcggtcggg gtattggggt ccggccgatg ggcatagaag agatcgacgt
     1501 aatcggtttg caggcgtttg agcgactggt ccaggctggt caggaggtac ttgcgcgaga
     1561 gccactcgcc atagggcccc ggccacatgt catagcccgc cttggtcgag atcagcagct
     1621 cgtggcgcgg catctccttg atgatctttc cgcagacgat ctccgcgttg ccgttgggct
     1681 cgccgtagtt gttggcgaag tcgagatgcg tgattccgag atcaaaggcc cggaagatgc
     1741 acttcttcga gacttcgtcg gagacatagc cgccgtaggt ttgccaggct cccagtgaca
     1801 cagcggggaa cttcacgccc cagcgtccgc agcggttgta gatcatcgag tcgtagcggt
     1861 tttcattgaa cgcggtcacg gtgaacctcc gttgttacgg cagtctgccc cgtcgccctt
     1921 cgccgggctc agggcccgag tcggtcgagg gcttgcgttc cgggttgcga gcaggattgt
     1981 ccgttccaag cgggatacct gtcaacccgg cggctcgcag atttcagcac cccgagcgag
     2041 agcgagtgga cgcaggtcgt ggaaacgccg cgaggcctgt cccctcgccg accaactttg
     2101 tactcgataa accggacttt gctggcataa gccatccatg acaacagata tgagcacacg
     2161 aagcggtttc tcacaaccgg ccctgcaagg gccggcccgt ccgcctgctt tcggcgggta
     2221 agccccgtcg aacggagctg aacgtgaagg gccccgtctg caatgcgacc cgctcgatac
     2281 ggaaaactgt gatgcccgac gctccgaaag cacgtcgagg ctgtcgtaca gacgcccgcg
     2341 gccatatact gatcgtgcgg aattcggcgg cggcggaacg accacggcca tgcgtgccgt
     2401 gcgaccacag tgaagatgcc gcccgacgag cgagcgatgt ttcaggagac gcaaacgtgc
     2461 cgatgattcg ttttgacgac aacccgctga tcactccggc cgacgtgaag ccctcgcggc
     2521 ccgattttga ggtggtgtgc gtgttcaatg cgggggtgat tcgtctcggg ggagagattc
     2581 tgctgttgtt acgcgtggcg gagcgacccc ggccgcagcc cggcgagttg ctggcgccca
     2641 tgctttgccc tgacgatcct tcgaagggcc ttgaggtgtt gcgggtgcgg agcgggagcc
     2701 cggatgtcga ggagatcgac tcgcgggtat tcaagtatca aggatcgacg tatctgacgt
     2761 cgatttcgca cctgcgtccg gctcgcagca aggacggtcg caatttcacg gttgcggaca
     2821 ctccggcggt gtttccggcg tgccgggagg aggaattcgg catcgaggat ccgcgcatca
     2881 cccggatcgg cgatcgcttc tacgtcaatt actcggcgat ctcgcggatg ggcatttcga
     2941 cggggttggc ggtgacacgt gactttgtga cctatgagcg gatggggatc atctttgttc
     3001 ccgacaaccg cgacgtgacg gtttttccgg agaagatcgg cggactgtat gtgtgctatc
     3061 atcggccggt gtcgggcatg ttcggccggg cggacatgtg gctggccacg tcgcctgatc
     3121 tgctgcggtg gggggatcac aagtatgtgg cgggtacgcg gccggggatg tgggattgcc
     3181 ggcggatcgg cgggggggcg gttccgttca ggacgtccaa aggctggctg gagatctacc
     3241 acggggttga tgacgcgcag cggtattgcc ttggggcgat gctggctgat ggcgagcggc
     3301 ctgagcggat cattgcccgg tcgcccgctc ctgttttgtc tccggaagca ccttatgaaa
     3361 gggagggctt tttcgggaac gtcgtgttca cctgcggggc ggttgccgag cctgatggcc
     3421 gggtgatcgt gtattacggg gctgccgatt ggtgcgttgc cgccgctgag acgacgatcg
     3481 acgaactgct ggcgagtgtt cggtgaagac ttggcgttga atcggggata gaatagattg
     3541 tggatgggcg tggggggtgt tgagcttgcc gaaacgactg tccgcggggt gtagactccg
     3601 gacagactcc acgacgggtt gcgggcggca gagaagacgg gaggcgggtt gtgactatcg
     3661 aactgaggtg cgaaagctgc ggcaagatgc tgcgtgccac gagcgagcgg gccggcactc
     3721 gggccaaatg tccggcctgc gggcacgagc tgtacattcc gatgccgccc gaccagatcg
     3781 aagagttgcc catgactccc gaggacgccg ctgagctgga acgggagcag gctttggagg
     3841 aagaacgacg ggcgctggat cgggctctga tgcgagagtc gatcagcaac gatgagccat
     3901 ctgcgcgccg aggggttcgt ccgccctcgc gtgccgggtt cggcggcggg acgggcgggg
     3961 gcaacgctcg cgaggccgtg gtggcttacc tgatggcgat gcgagatgcc aaccttgatc
     4021 tggcggccca gaagctcgcc gttctgcggc gtcagaaggc cgaggctctt gaggtggtag
     4081 accaattggc gaccgatcaa ctgccgccgc cgagcctggc caatgttccg cccggtgttt
     4141 atcagggctt tctgcgaacc ctacggtcac agctttgatt tgccgcgagt gaggcggtcg
     4201 gggaccaggc cggaatgaaa gacgaaaagc ccgcgaagcc gtgaggcaag gcgggctttt
     4261 tgattgcatg cttgggcgac tgctggtaac cggtggtctt tgcctgtcgc cggttgggag
     4321 ttcagaggga ccgaggttgg gccggcgttt tgtcggacta ccgacggtct ggaaaacccg
     4381 ttgttattgg cgcaaggagc ttccggcgca atgccgtcgc gctccgcctt gggtcgcggt
     4441 ctatttcacg cctggggggg tcggcgttgg ttccatctct tcctcttgtt cctcttccgg
     4501 catggtcatt ccgggtccgc ccatgccacc catgaacatc atcatcatcg gcttgaccat
     4561 ctcggcggtt gactgggcca actcgatcgg gaccagcacg ttggcctcga cggcggtcgg
     4621 ctcgacctgg gtaaccatga tcgacaaagg agcggcgttg ctcatgttca aggggatggg
     4681 catcatttga ccggtcttgg caaagacatc cgagatggtc gagagcaact ggtcgacgct
     4741 gaggtaccct tcgccgattc gtttggtggc cgggaggcga tcggcgatca tcttgatggc
     4801 cttgcggtcg gccaagggcg cctgattctt ctgtaccatg tcaacgacct gggcaaaccg
     4861 cttggccccg ccgccgaagg taatcgcgac gtttctttcg ccgacggagg cgatgcggat
     4921 gatgatgcct tcctgtccga ggacgttctt gaactgctcg agctgctctt cttcgagatc
     4981 ggggatcttg gcgaggtcga tcacgaagtg atccacggcc gcaccggtca gtttctcggc
     5041 gttttccttg agctggatcg cctcgtcgat ggtcttggag tcttcctcgg tcagcgagcc
     5101 gttgcccaag gccgtcttga cgagagtgtc cttgacggtc ttgaagagtt tgcggatttc
     5161 accctgggcc tgttggctgt tggcgacttt ggcgaccaag gtggcgccga tcaggccgtc
     5221 ctggctctcg ctgggcaggc tggtgacgct gacggcggcc tgctcgaacg aggttgcgag
     5281 tttgacgagc gattccttga tgctcttgat ctgctcttcg tcgacgtcgg tgccgaccat
     5341 ctcgggcttg aggatctggt ccacgacctt cttgacatcc tcatctctga gtgtgttcat
     5401 ccagccgccc gcggcgatca tgggctcgtc ggggagaccg acgaggaggg atccgtcgcc
     5461 gggcttgacg gccgacaggc gtttggcgat gtcggaatcc ggcgtcgcct ggtaccagaa
     5521 cgtcatcttg agtccggtct tggagtccag agtcaaggag aaagatgctt ccttcacctc
     5581 gtcaaggaac ttggtgattt cggcgattga gtcgtcgatt tcctcgggct tggcgccggg
     5641 ggtgccggcc atcatcatgc ctttgagggt gttgatcggt tcagcacgaa gctccttgga
     5701 aatgcgtcgc atgccggccc acgcgaagag gtcggcgtcg ccataggcct tcagccggtc
     5761 gggggccata gccttgtcga tgccttcctt ggcctgaacg gcttccttga gggcgtccgc
     5821 agcctcggcc gatgcgatga ccaagaagcc gcttttctcg gcggcgatgg ccggttcgga
     5881 tccgagggta agctggtatg tatcgccctc tttgtcagtg ccgcccaagg ccttgactaa
     5941 ggcatcaggc ttatcagtgg gcaggaaaat caccgcccgc ttgtgtactt cgtcgacttt
     6001 cttgacgtcg ctgcaatcca ggagagcaac ggccgctccg gagttgagct ccagcccttc
     6061 gtcgatgccg acctgctctt taatcatggc gaccagcccg gggaagccca actgatccag
     6121 aggcagttcg agcttggcca gaatatcggc gacgtcgtaa tcgacctcgg cgatatttcg
     6181 gacggccacg aacgcggtcg cggaggcggg gatagccttg aggaccgcgg tgggatcttt
     6241 gggcggggcc ggcttgaccg gtttggcgga ggtggccggt ttctcgccgg ccggggcttg
     6301 ggcgggcagg atcgtgcccg gtatcgccag gaggagcgag cacacgatga gattgatcat
     6361 ccgctttggt tgtctggttt gcatgctgtt gttccttatt cgagggtcag tttcggcacg
     6421 ttcatggcta tacccgttcc tggggtaatc gtcaagccaa ggcgggccat ggtcctttcc
     6481 gattagacgc cacccgatcg caaaagttta gccgctccaa atgaaaaaag cacttctcgc
     6541 acaaaagaag ccgtggaggc atcgacaggc ggtctttttc tgatcttctt gcctgggcgt
     6601 tgcggagtga tccgaagaat tcttcgcaca atcctcccgg caggcgcgtt tttcaccctt
     6661 gttcatcaag agctgttccc gccggcaaac cgcttggttt gtcggggcca atgaggttga
     6721 tgaaggagac cgttggtggg cagaggaagc ggatttgcgg ggcggagccg cgttccatgc
     6781 cgatgccgcg gttgacgtag agggggtgtc cgttgatgtg gaagaggccg ccgccggccg
     6841 cccggcgggg gatggctgat gatcgcgcga gagcgccgac ccagggcaaa cggacctggc
     6901 cgccgtgggt atggccggag acgacgaggt cgacattcga ttgtggagag aggtgaagaa
     6961 cggcgtcagg ccggtggctg aggatgatgc ggatgtcgcg gggagtgccc gtctcctgca
     7021 agcgtctgat gacgttttct tccgcgcgtc cgaggtaggc tccgctcaag ccggcgatca
     7081 ggagtcgccg gtctttgaat tccacggtga tgatctcgtt gtacaacagg cggacgccgg
     7141 ttccttccat ggttcgcttg acccaggaca ggtaatcaag gttgccgagg acgcaataga
     7201 cgccggcggg ggccgacatc gaggcgtgga gctcgcggaa cgcggggatc tggcgttcgc
     7261 gctcggccag gttggcctgg cagacgtctc cggccagaag gatgatgtcc ggccgtcgtt
     7321 ccatgagcat ccggacggcg gttctttcgt ggtcggtgac ctggggcgtc tggaaatcgg
     7381 agatcacgcc gattcggagg ggcgccaagc cgtcgcgatc gtcgggcaga ggcaaggtga
     7441 cggtctcgat aatgagccgg tagggctcga tgaagctggc gtagaccccc acgagcgcgg
     7501 ctgacagtcc caggagggcg acaaccaccg aaggtcgggt ggcccgcaga aaagatcgtg
     7561 gccatggaaa ggcggcaatc aacaagccgg cgctgaacag cggcaccgca aagaccattt
     7621 ccgtgtagac ggcgttcaac aggccgaaat gatcccatcc gcggcgatga aggagcacca
     7681 gtttgagggc gcacgcgccg acgaggactg tcccggcaag cagggtggtt gtgatggtga
     7741 cggccgtttt gcggttcttg cggcggcgga tgccagccaa ggccagcagg acgacgaagg
     7801 caatgccggc gtcgattgcg gccagtttca ggcaggtggc cgtggcgtag gtaagcgaca
     7861 tctgcaagac gatactcgct atgggagagc taatccaggc acgttcgtct cgttgcgtat
     7921 tctacgctcg cggggggctc tggttctgcg actttccgga tgtggcgatg gttgccgcga
     7981 cgcgccggcg gcggtaccgt gtccgatatg tgttgcaggg ggttgcgatg ctgtattccg
     8041 gtcgttctgg cggcctgcgg ttgtgccggt catcaggcgt cggctttgcc cgcttttccg
     8101 ggggccgagg ggtttggtgc gcagacgccc ggggggcgtg gcggccgggt cattgaggtg
     8161 accacgttgg acgacagcgg gccgggatct ctgcgggagg ccgtatcggc caaaggcccg
     8221 cgaacgatcg tttttcgcct tggcgggaca atcaggctga agagccacct tcgcgtgacc
     8281 gagccgttcg tcacgatcgc cgggcagacc gcgcccggcg gcgggattct gctgcgagat
     8341 gccgggttgt tcatcgagac gcatgacgtc gtcgtgcgat tcattcgttc gcgcgtgggg
     8401 gccagcctgg cggagccgta caacacgcag gatgcgttgc agatcggcgg aatggacgct
     8461 cgtgacattg tagtcgatca ttgttctttt tcatggagca tcgacgaatg cgtcgggatc
     8521 cgcgcgccgg cgcatgatgt gacgttgagt tggaacatta tgagcgaggg cttgaggcgg
     8581 ccgttcagcg gggagcaaat cggggaggat cgttcgcaca gcatggcctt gattctcagc
     8641 ggcggtccca cgcgttgctc gctgcatcac aaccttctgg ccctgtgcaa cagccgtaac
     8701 ccgcgcatcc agggcgggcg tcatgcgttc ttcaataacg tcgtctatgg ctgggggtgg
     8761 ctgacgggta ccttttctcg aaaccccgag gtcaacttca tcggcaatta ctacaagccc
     8821 ggtcccggca gccaggcgat caaggcgatc tgcgagcggc ccggtgagat gggaaggatc
     8881 tatgtgaaga ggaatcgcag tcccgaccat cctgatgact cgtcggggga atgggagtcg
     8941 atggtgaatg gttcggcggc ggagcatcgg gcgtccgagc cgtttgagat gccgccggtg
     9001 acgaccacgt cggccgagga ggcttatgcg gcggtgttgc gttttgcggg agccgttctg
     9061 ccgacccgcg atccggtgga cgcgagggtt gtgcggaacg tccggttgtc tatgggagag
     9121 aagatcgacc gtcccgagca ggttggcggc tatccggaga tggcgtcggg tacggcgcct
     9181 cgcgattcgg accatgacgg gatgccggac gattgggaga ctgcgcaggg atttgatccc
     9241 gccgaccgcg atgacgcaat cggggatgct gacggcgacg ggtacaccaa cctcgaggag
     9301 tatcttcaat tcatcgtcga agaggggttg acgccacggc cggaggtacg aggtgtttcg
     9361 atagacaggc cgggcggacc gttcgtcgtg gagtgcgagg cgggccatct gcccgtcgtt
     9421 cgcgtgggcg gagacgagcg gtcattttac acgcacgcct ggtttcagga tgaagtccgg
     9481 ctgcaggtca agccgggtgc cggcggcgag gtcgtctcgg cgcaaccgtc gaggcttcgg
     9541 gaccgactca agacgtcgga gaacggaatc gagctttacg tggcggagga gggcacgagg
     9601 attttcgaac agagcccgga cgggcatcgg ctcttcgctt tttttgacgc gtaccccggc
     9661 aacgcgccgg agttggaggg agacaacgtg atcgacgcag gccggttcgg cgttgtcggc
     9721 aatcgtgaca acgtgaccgg caagctgcag gcggcgttgg atgcggcgtg caaggtgcgt
     9781 ggtggggttg tgttcgttcc gagcggcgac ctggaggttg acacgatccg cattcccagc
     9841 ggcgtgacgc tgcacctggc ggtgagttcg gttgtgtaca gcagcggccg atcgacagat
     9901 ggggcgatga tcctgtttga cggcgtcgag gatgcgcgac tgacggggcc gggagtgatc
     9961 gacggccggg gggacgaagg gttgccgccg gtggcggcgg tagccgttcg gaatgctcgc
    10021 tcgattgtcc tggaagatct gctcgtgcgt aactcctccg gtttggggat cgaggtttcc
    10081 gatagccgcg acgtcaaaat caacaggtcg aagattctgg ccgtcggcaa ccgtgagggc
    10141 atcggcggtc tggacgtgaa tcggtgtgag tcggtgacgt gtgagcggtc attcatttcg
    10201 agcaccgggg ccggcattcg cattcggtcg gccggtgtgg gtggggccgt cggtcacact
    10261 cgaggaatac gggtccgaga agcgacggtg ctttcggcag ggccgggcat cagcgtaggt
    10321 ccggaaagcg atcaggtcgt tggcgacgtt gttgtccggg acagcgacct gttctgcagc
    10381 ggagaaggca tatcggtggt ggccgcggac ggtgcggggg ggatcgagac ggcggtgttc
    10441 aaggatctga cgttggatgt gcggccgctt gcgggcgccc ctgagagcgg acgtccgttt
    10501 ctgatcctga atcggtcgga aggtccgatg aggaatattc ttttcgagcg tatccgggcg
    10561 ggtgcctggg ccacgtcacg gattgagggt ttgggttccc ggccaataga ggacgtgaag
    10621 ttctggggcg tcgaggtgac cgcgaaagat atcgctcgtt cggagccaca gccgctcttt
    10681 catgttcgac acgcgcgttg tccgcagttc cgcttactat atgtatcctg gccggcgggc
    10741 gggtcggcgg gctggagcgg cgtcttccgg ttcgatgatg tctcggacgc cgtggctccg
    10801 ccggcagagc tattcgagag cactcttcgc tgacggccga atgcatcgtc gcattatccg
    10861 gtccgtaggc gatatccctt ctgatgcaac cgggtttgcg cgacgatgat gtttatcgtg
    10921 cttgcctgcc aggtaagaag ttccgagtcg tcagaagcgt gataatggtg gcggacgttc
    10981 ggttgaatct cggacgcatg tgaagatggc gaatttcttt tccggccggg cccataagac
    11041 gggcattcgc agcttgtggt cggcacttcc gatatgtgta tgccaatctg gtgaggtttt
    11101 tttgcggcgt gcgtttgcgt gcggtttttt tgtttcttat gtttcatgag ccgttcgacg
    11161 atcacgagtg cgggaagccg agaggggttc tcgcatggaa ggcgggaaca agcaagacct
    11221 gccggtcctc gcaacggagc ggcccgcacg aggtgagatg agaaggcggg ccggaacagg
    11281 aaacgattgg agcgtgtcac cggcaggagg atgcgccggt tttcagtcgg cgaaatccga
    11341 cgtgtgatgg ggaaataggc tttgccggcg gagggaaaga tgccggcggg cgatgctttc
    11401 cgtaggggta agcaaaggag acgtcatgaa gaacctgatc aatagagcca aggcgttcat
    11461 taagaacgag gacggcccga ccgcgacgga gtacgcggtc atgctggccc tgatcattat
    11521 tgccgctctg acggcgatta ccgccctggg cggccgggtc acggagatct tcactgaggt
    11581 caccgagggt atcggcgggt aatgcatgat gctggaccgc cgtgacaacc gcggcggccg
    11641 cccgcgtccc ggtttcgcgc ggttggttcg aggcctcagt ctcccgccgc gaacaggaag
    11701 agccgggacg acgtcaggat gacgcgagac actgatgtgg gcgtccgtcc agggtgggtc
    11761 ggacgcccac acctgcttga ggggacaggc ggtgagggtt gcgacagtgg agggtgctga
    11821 cggcctcgcc gggtgaaagg ccggcgccga agggcaacgg agcgaagaaa agatgtgtta
    11881 tgagtactgg ctgacggcgt gtgcgatcat ggtcccgggg atcttgtggg cctcatggat
    11941 cgattacaag gagcgccggg tgcccaactg gcttaatgcg gccatcgcct gtgcgggttt
    12001 tgccgcccag ggctggtttt tcgggtggca gggcctgggg tggggcgtgc tggggttggc
    12061 ggtcggtttc agtgtgctca ttctgccgtg gttgatgcac ggcatggggg cgggtgacgt
    12121 caaactgatg atggccatcg gcgtgtggct cggcccggcg ctgaccttat ggtcgttcct
    12181 ggtcggagcc ctggccggcg gcgtcatcgc cgtgatcatg atcgtgagca gcgggcggct
    12241 gtggaacgcc tgggcgaaca ttgccacgat catgcacaag ctgtccaacc gctcaaccat
    12301 tttcagtgaa ttcggttccg ccaggagttt cggggagaca tcgcagctct tgccgtacgg
    12361 tgtgccgctg accatcggca cgttgggcgt tttcttcgct caactggcat tgatgaacgc
    12421 ggcgtaggag ggcgaagtga tgacacgcac gcacaagggc cataggagcc ggacgttgag
    12481 ccggcggttt cgccggggag cggcggtcgt cgagatggcg gtggttgcgc ctctgttgtt
    12541 gacgctggtt ttcggcgtga tggagttcgg atggagtttc atggtgcacg aaacgatcac
    12601 gaacaccgct cgcgagtgct gtcgcctggc gacgttgcag ggcagcacgg acgccgaggt
    12661 ctacgcccgt ttcatcgagg cgatgtcggg cacgggcgtc aatgtcacca cggatatgat
    12721 cacgatcaat cggtcgccag acgtggacaa cccggtcgtg accatcagca ttgcggtgcc
    12781 gtattcggag gtttcgatca cgggcttggc gtcttttttg ggtatctcga cgcagaatct
    12841 gagatcgacc tgctcgatgc ggtctgaagg cacgttctag cgtcgcgcgg tccagcggag
    12901 ctgtccgaag accgggcggc tacggggggt gcgcaggagt taaggcctca agggtcaggg
    12961 ggacacgaca tggcgtcgtt ccctcacgag catatgtggc aaggccggcg gcaaggaccg
    13021 gccggttccg ctcgctcgtg ctgcggaagg agcatcagat gatgaagagt cgcgcagtta
    13081 tcccactggt cgtgggactg gcggtaggcg gcattgcgat aaagatcttc ttcaacgttc
    13141 tgaagaaggc gaagggatcg agtgtgtccg acacggttca ggtcgtacgt gcggcggcgg
    13201 acatcgcctc gacggtcgag atcagtgagt cgatggtcgg actcgtggcc gtgcccaagc
    13261 cgctgttgcc ggagggctgt ttcaccgatc ccaagcaggt cgtcggccgc gtgagcagcc
    13321 agacgattcc caagggcatg ccgataactg ccagtctgtt ggccccggag ggcactccgc
    13381 ccggcatggt ggccaagatc caggacggat atcgggcggt cgcaatccaa gtggatgaag
    13441 ttgcgggcgt cggcgggttc gtcaagcccg gggctcgcgt ggacgtggtc atggtcgtcg
    13501 ccggccaagg caggaactca aacatcagca gggtgatcct gcagaacgtt gaggtacttg
    13561 cggtggggca gaagaccgag gtcggggccg agggcgccgc ggtgacgcgg tcggtcaccg
    13621 ttctggttaa gcctgaggag gcctcgaagc ttcatctggc ttcgatgaaa gggaaactgc
    13681 ggttggcgat gcggagccag gaagaccggc gcgagccgaa accggtgaca ttgacggacc
    13741 aggacttgtt aacggacggt cctccgcgga acgcgaagcg gaatgaggat tcactacttg
    13801 cacggctgtt cggcaatttg cccaagacgt ccaagtctcc ggacgatgta agaaccgggt
    13861 tcaaggcttt gccggctccc gagaacaagg aatggacggt cgaggtatta tcgggcactg
    13921 agacattcaa ggtgaagttt gattccgagg ggaaaagcaa ccatccggtg tccacgggga
    13981 gacgcaagcc gaacaaggat ctgagtcact caccttcggc ggacgtggcc gccgagcggg
    14041 tcgatgaaca gcctgacgcg gtcgagtccg ggccggtacc ggaatccgcg gccggcggtc
    14101 aaggggccga cgtcgagggg cacgcgagcg tgttcgacaa gccgagcaac gaatgatctg
    14161 gacacgaaaa aggggacacg aggatgatcc ggatgaatgg tcgattcaaa gacatcttga
    14221 cgtgtgacca accgcgcccc tggctggcgg tatgcgcggc ggccgtcctg ttgtgctcga
    14281 cggcgggcgg ccagagcacc gggaccaccg acgccctgat cgtcggccaa tcggccgtcg
    14341 agaaggcgtc gccgctggtg ttgagcaacg gccagggtcg cgtgcaggcg acggtgcacc
    14401 gcgacgacct tgccgaggag cgcgtgaccg tcacggtggg cagcagcacc gtcgtggaca
    14461 tcaacgtacc ggtgaagcgg gtcgaggttg ccagtccgga gattgccggc gtgaccgtgt
    14521 tgtcgccgaa gcagattctg gtgtcggcgt acaaggtagg catcacgcag atcatcttgt
    14581 ggagcgaaag cgaagatcgg ctgctgtttg cggtcgaggt cgagccgaac atcgcccaga
    14641 tcagggaggc aatcgccaag ctggtgccgg atgccaaggt ggagctgcgc gtggtgaacg
    14701 gctcggtggt ggtgagcggg cgcgtgtcca acgtggacga tgccgcgaga atcacggact
    14761 tgtgcaggat ttcgggggcc aaggtacaaa accagatgat cgtcgccggc gagcagcaag
    14821 tgctgcttcg ttgcacggtg gcggaagtca gcaagacggc catccgggaa ctgggcatcg
    14881 atgcgtgggc gtctttcgag gccaacccgg cgcagaccgt gcgggagatg atcgggctga
    14941 ccgatccgtc ggcgctgctg ccgtcgcgga attatgtgac cggcacgtcg aactttttcg
    15001 ggaatcgttt cctcttcggt tcgacggagc agggcgcggg ctttgcgatc agttcaaaga
    15061 gtctgcagat ggagatgttc gtacgggcgc tgaagaggaa cgggctgatg cgcattctgg
    15121 ccgagccgaa cctcgtggct ctgaccggtc agaaggccga gtttctggcc ggcgggcagt
    15181 ttccggttcc ggtaccgcag cgcgacaacg tgaccatcga atggaaggac tacgggatca
    15241 agctggcgtt tgttccgacg gtgatcggcc aacagatgat tcgcctgtcg gtggcgccgg
    15301 aggtgagcga gctcgactac accaacgcgg tgacgctgga atcatacgtc gttcccggcg
    15361 tctcgcagcg gcggacgatg acgaccatcg aattggccag cggaagcacg atcgcgatcg
    15421 cgggcctgct tcgcgagcag attcgaggag aggtcaacaa gttcccggca ctgggggaat
    15481 tgccggtgct gggggcgctg tttcgctcgg ttaattatca gcgcgatctg acggagctga
    15541 tcattctggt gacgccggag ctggtgagcg ggatgatgcc cgaccaggtg ccgggcgtgc
    15601 cgggcaagga cgtgcaggac gtcagcgatt ggaacctgtt tggactggga cttttggaag
    15661 gcaagccgat gccgcaggac aaggaaagag aaggggcgct gctgaccgag caggcgcctc
    15721 acgtccgcaa atttgccagt ccgccggacc agatgtcgat ccacggcccg tggggggcgg
    15781 cggatccgtc tgaggcggtt gtcaacagat agagtgtcac gggggttcac ggaggagcat
    15841 ccgtgtggga aacgagggaa ggagcaaggt ctggccaacg gcgcctgagc gatggaaggc
    15901 cagcctgccg gggggcacgg gattaggagg gtgaaatgtg tggctcaaag agactagacg
    15961 gggccatgcg ccggcgtcgc catcttcggc gggggcggcg gggcgcggta gctgcacaag
    16021 tggccgtatg ccttgtggtc ctgctcggat tcaccgctct gactgtagac gtcgggtatc
    16081 tgtacaacgc gagggctgag ctccagcgta cggccgacgc ggcggcactc gccgcggtgc
    16141 aggaactggg cggcggcaag agcgatgtgc tggcgatgca gagcgcccgg cagacggcgg
    16201 cgaactatgc cgcagccaac accgtgctgg gacaggcgtt ggtattggaa gagtcggacg
    16261 tggttttcgg gcgagcgtac cttgaagagg ccggagcgaa atacacattc gagcccgggg
    16321 ggacacccgc gaatgccgtt cgggttcgcg cccggcgtac ggcggactcg cccaacggcg
    16381 gggttccgct gttcttcgcc cgtattctgg gcatatctca aagcgacgtg agcgcccagg
    16441 ctacggcggc cctgacgccg cgcgacgttg ccgtggtgct ggacctgtca gcttcgcaca
    16501 acgacgacag ttcgcttcgc gcggtgaaga aaacagagat ccacaactat gaggtatgga
    16561 gcgggctgaa ggataacaac catgctttgg cgcccaagac cgactcactg gggtttacct
    16621 cggttgtcga ggttaccgac aacggtgacg ggacgtccac ggtgaccgtt cacctgacca
    16681 gcgacggcct ggaagagacg gcggcgttga gtcacgtcac tttcggtttg ccggaagatg
    16741 cgtggtcgat ggcacaaagc accgcaacca gcggcggagg ctacccggta tcgacgggcg
    16801 tcgatccgac cacgggtgtt gcgggcctga agtttgacga gacctcgctc ggcgaggacg
    16861 gggccgtcga gacggaggac ttctccttca aggttcccaa tgactgcttg aaatacctga
    16921 tggtcgtggg gaccaaggcc ggtggcgagg ccgacacgtc ggccaagtat aacctttccc
    16981 cgggaccgct cctgggcaac atgaacacct ggggagacgc cacggcgggt ccgagctgga
    17041 accctgcgtc cgacgccggc ttggtgaagc tgcaaaaggg cgcgaactgg agcctgagca
    17101 gtgcattcgt cagccagacg ctgaagatgc gcggttacgg gcagtataac gccgcggaga
    17161 tgtcggtgat caacagctcg tcgggtgaca gcaccacggc cgcatacaaa cggcgtgttc
    17221 gggtcgcttt gggacttgac cgctggaaga gcggcaagcc gggcggccag ccgggcggca
    17281 acggggacaa cgtcatcgac gccggtgaag tcgttcccat ggttgcctat ccgagccaga
    17341 acgtgaaccc ggacagctac agcaagaagg taggcggcac gtgggacagc tatattgact
    17401 acgtgatcag cagcagttcg gggatgtgcg tttatgaccc ggcgaaggag tattacggtg
    17461 atcccaacct gcggtacagt ttcgggttga agacctgggt cgactatctg caggaatgcc
    17521 aggttggcga agcggcgtcg cccggcatgg cgggtgttcc gacgcaaccg atgggggccg
    17581 tggcagacgc ggtgaaggag tgcgtcaaca tcatcgcagg ccttgagggt gacgatctgg
    17641 tcggcatggc cagttacggt accgtcagct acgggccggc ggacaagccg aacaacatgt
    17701 cctggctggt ggatgatttc ccgagcttgc tggccaaggt ggatactctg caggccggta
    17761 tgtggaccag ctacaccaac attgcccaag gcattgatga gggtgttgag gtgctgttca
    17821 acagccccaa cggtcgaaga aacgccgcca agatcatgtt gctgttgact gatggtgttg
    17881 ccaaccagac gcgcaacccg gtgtcctata acacttacca ggctcgtgag gacaccaagg
    17941 cggcagctcg tgatgcccgg gatcgcggcg tccgcatcta cacggtgagt gtgggtgttt
    18001 cggcggacaa ggatctgatg gcggaagtcg cctcgatcgg cggcgggtcg tggttccatg
    18061 cggagggcga tatcgagtcc tataaggacc aattgaaggc catcttccgc gaattgggcg
    18121 gcaagcggcc tggagtgttg atcgattgac gtgtctgctc cgcgcccggt gccgaaggcg
    18181 gcaccgggcg catgagggtt ttgtcagatg gcaggcaggg ggatgcgcag tcgggacgga
    18241 cagggtcttt ggcaagaacc aaccggggcg ccgggcgacg gcgccgccgg ttggtttgat
    18301 gcttggattt ggtcttatca gacggcgtat cggctggccc ccggctcgag ggagcaatga
    18361 ggcagtctgt acgcgttatt gtgttcaacg ccgatgagga gtactcggcc gcgcttcggg
    18421 ccgaccttct gagcattccg ggcgttcaga tcgtcgccga ggtggatgag gcgacgctga
    18481 ttgagcaggc cgtgacccat tttccggccg aagtggtggt gatccatctc gacccgatgc
    18541 ccgaggcgct gctgcccgtc gccagcagcg ttgcaagcaa ccatcctgag atcgcggtgt
    18601 ttgtcgtcac ctcgagcacg gacgggcaat atgtactggc ggccatgagg gcgggggtga
    18661 aggagttcat caccaagccc gtggatcgtc aactggtggc gacggcgctg gacaaggtcc
    18721 tcgcccagaa cgagtccgcc catgagcgcg gcgtgctgat ctcggtgatg gggacgatcg
    18781 gcggctcggg ggcctcgacc gtggcgacga acctggccgt cgaactggcg gagttcgcca
    18841 agaagaagcc tgtggccatc gtggacctgg acttccggtt cgggcagctc agcaccatgc
    18901 tcgatctgca gcctgactac acgattgccg acctatgcga cacgccggaa aagatcgacg
    18961 cgggggtgat cgaaaaggcg atggtcaggc acaataccgg cattcacgta ctagcccggc
    19021 cgaatcactt cagcgaggcc gaccagatca ccgccgcgca ctgtgtggcg gtgctcagct
    19081 cgctgcagca gatgtatgaa tacgtggtcg tcgacgggcc cagtcggtac gacccgggcg
    19141 gcctggccgt gctggacatg gcggacatga acctgctcat catgcagctt ctggtgacca
    19201 gcgtgcgaaa cgttcaccgg atgctcgatg agcttcgcgc gggaggctac aacatggacc
    19261 ggttccacct gatctgcaat cgggtggagc gtgattcgaa caatctcgag atcagccacg
    19321 tggaggaaac gctgaagaag gacgtggccc accagttgcc cgacgactgg aagaccgtga
    19381 gcaccgccat caatatgggg gtgtctctca aggagtactc cgcaaagtcg aggattcggc
    19441 tggcccttcg tgagcttgct cgccggattg cccagccgga gaacgcggac gaggctcatg
    19501 aaaacggcgg caagggcggc ctgttcagcc ggctgctgaa cgcggtgtga ccggagcgtt
    19561 cgccgttgtg atggaaggca aactacggac acggggatga gacatgtttg gtaaagtcag
    19621 gacacctgcc acacccaaga tcccgcccag aaccgagaag ccggagggcg gaaaaacgat
    19681 tctcgggccg cggccgccct tgtcggggac gcagccgggc ggcggcgtcg ccgtggagga
    19741 cgagcccaaa cccgcgcctg cggtgcagcc tgacaaggcc aaggttcgcc aggccgacga
    19801 tgacaagctg gagaagtact acgccctcaa gacgcgaatc caccgcaaac tggtcgagca
    19861 gctcgatctc aacgccattg acaatgatga cccgaccatc cgcgatcagg tcggggaggt
    19921 cattctctcg ttgtgcgaga aggaaaacgt tctgctcaac cacaacgagc ggcagcaact
    19981 ggtcaaagag attcttgatg agacgttcgg cctgggtccg ctggaggtgc tgctcaagga
    20041 ttcgagcatc agcgacattc tgatcaacgg cccgcggcag atttacgttg agcgcaaggg
    20101 cgcgctggag ctgaccgacg ttcagtttcg cgacaacgag catttgctgc acattatcga
    20161 caagatcgtc agcgcggtgg ggcggcgatg cgacgaggtg tgcccgatgg tggacgcccg
    20221 gttgaaggac ggatcgcgtg tcaacgcgat cattccgccg ctggcgcttg acggtccgag
    20281 cgtatcgatc cggcggttcg gaaacgaccc gatcacctgg gacgactaca tccggttcaa
    20341 gtcgtgcgcg cctgagatgg tggagttcct caaggcttgc gtgatcgccc atttgaacat
    20401 cgtcatcatc ggcggtacgg gctcgggaaa gacgacgctg ctgaataaca tgtcgtcgtt
    20461 cattccgccg acggaccgta ttgtaacgat cgaggatgcg gcggaactgc ggttgcggca
    20521 gccgcacgtg gttcggctgg agacccggcc ggcgaacatc gagggcaagg gtcggatctc
    20581 gattcgcgac ttgctgatca acgccttgcg tatgcggccc gaccggatcg tcgtgggcga
    20641 gtgccgcggt gcggaaacac tggacatgtt gcaggcgatg aacaccggtc acgacgggtc
    20701 gctgaccact ttgcacgcca acagcacgcg cgacacgatg caacgcgtgg agaccatggt
    20761 catgatggcg ggtttcgacc tgccggtgcg ggcgattcgg cagcaattcg cgtcggcggt
    20821 ggacctgatc gtgcaggcgc agcggttgac cggcggtccg aggaagatcg tgagcgtcac
    20881 ggaagtcgtg ggcatggaag gcgacgtggt gaccatgcag gacatttttc agttccagca
    20941 gcttggcatc gatgagcagg gcaaggcata cggccagatc gtctcgacag gcatccggcc
    21001 gagttttctg gaccggctca agtacgccgg atgtgacgtg agcccgtcgc tgtttgagcg
    21061 gcaggtgttg gtgaaggata aggatgaata aagctgatgg agagaaagcc gctttgccgg
    21121 gcggcgcgat ccgagagaac ggaacggggt gaccagtgga atcgttgtca gctttgttgt
    21181 tcttgattat gccgcttgcc ggagccacgc ttctggcata cggcatcttt caggtcgtga
    21241 tggacctccg gggcggggag cagcggaaaa tgctcgatcg cctgagcggt gcgaagtccg
    21301 tcgaccgcga gctgaaggtg cgggaatcgc tgcttcgcaa gaccgccgcc gagactcagg
    21361 ggaacttcct cgagtcgctg gtcgcaaaac tccatctggt aaagaagctg caatccgttc
    21421 ttgaccaggc agaccttggc tggtcggcat cgaaggtgct ggtgaatctc gcgggtgccg
    21481 cactggccgt ggtgctggtc atggttctgc ttcagatcag tccgctggtg atccttctgg
    21541 tcagcgtcgc catcttcgga cttcccattc tgtacatgat ctggcagcgc aagcggcgca
    21601 tcagaaaact ggtcgagcaa ctgcccgagg tgttcgatct catgtgtcag gccctgaagg
    21661 ccggtcattc gctcgccagt gcgatccagt tgatcagcca gcagatgcct gatccggtcg
    21721 ccggcgagtt tgcccgcgta ttccaggaac agaacctcgg tctgaccgtt gaagacgccc
    21781 tgttgaacat gacgaagcgc gtggaccaga tggacgtgcg gtttttcgtg accgcggtgc
    21841 tgatccagcg tcagacgggc ggcgacctgg cggaagtact ggagaagatc ggcaaggtga
    21901 tccgtgaccg catccagttg ttcggcgtcg tcagggccct gaccgccgag ggccggttgt
    21961 cgggttgggt cctgctgctg ctgccggttc tggtgttctt cgggatgatg gtggtcaatc
    22021 cggggtacgc cgcggaactg acggacacgg attcgggccg gaagatgctc ggaatcgcca
    22081 tcggaatgga cctcatgggc atggcgatga tcaagaagat agtgaacatc aaggtgtgac
    22141 accatgagcg aaatggtact gttcggcata ctgattgtgg cgagcgtcgg gctcattgtg
    22201 tattcgctgt ggcccgccaa gaaggcggag agcgatcaca tccggcgtcg catggccggt
    22261 cgacgcctgg acaaggccga ggccttgctc accgtggcgc ccaagcggga acggtcggcc
    22321 gcgaaggaaa tgatcgagaa ggttgctccg attgcgatgc gaccggtgat gccggccagc
    22381 gaagaggaaa tgaacaagct gcggagcagg ctgtctcagg ccggatatcg cagcgaggcg
    22441 gccctgcgat atttcctggc cagcaagacg atcatggggg gcggcgttct gctggtggcc
    22501 ctgctgtttg ccatgggaat ggggcttgag accaggcaga ttttcggcat agcggctttc
    22561 gccggcggca tcggcttcat gttgccgaac ttctggctct cactcgcgca gagctcgcgt
    22621 caggagagaa tccgcaacgg tcttcccgac gtgctcgatc tgatggtcgt ttcggtggag
    22681 tccggtctgg gtctggatgc agcgatcatg aaggtcggcg aggacatgca caacgtgcat
    22741 ccggagctgg ccgaggaaat gatgatcgcc gtggcggaag gccagatggg cctgcctcgt
    22801 atggaagccc ttgagaaaat ggccgcgcga tgcggcgtgg acgaagttcg cgcccttgtt
    22861 tcaacggttg cccaggccga gaagtttggc acaagcatcg ccaaggcact tcggacgcaa
    22921 gccgactcgt tgcgggtcaa acgacgactt cgggccgagg agcgggccca gaagaccacg
    22981 gtcaagctga tggccccgct gattctgttc atcttcccgt cgatctttgt ggttctggcc
    23041 gggccggccg ccatgaagct ggtcgcggca ttcagcaggg gaggcatctc tggggcgccg
    23101 cactaattga gacggcggcc tgctcttggg accgatggtg attcaagaag acgagcgggc
    23161 ggtccgtttt tcagaccgcc cgcgtttttt ctgtcgccag ggggccaaac ccgaccagcc
    23221 gggccgcgtc ggatctcaaa gaccgtattc cttcatgact gccaggaggc gctcacggtt
    23281 cgccgggctc atggggcaga gcggcagacg gaactcctcc gtcagcatgc ccttgatggc
    23341 catggcggtc ttgatcggga tggggttgac ctcaagcgtc agcaggccct tggccagctt
    23401 gaacatcttg agatgccatt tgcgggcggt ttcccagtcg ccgcgcaggg cggcatcggt
    23461 gagggccttc acgtctttgg ggacgatgtt ggccagcacg ctgatcacgc ccacgccgcc
    23521 gatggacatg agcggcaggg tcatggagtc atcgccgctg atgatgtcga tatcggacct
    23581 gacgcggagt tcgctggcgc cgtccattga accggtggca tgtttgacgg ccacgatatt
    23641 cttgtgggtc tctcgcagcc ggacgatggt atcgacgctc agctccgttc cgcatcggcc
    23701 ggggatgttg tagagcactt gcgggaaatc cgccgccttg gccaaggtac tgaaatgctg
    23761 aaacaagcct tcctgggtgg gacggttgta gtagggcacc accaggaggg ccccgtcggc
    23821 cccgttctta agggcgtttc gggtcataac caaggcctcg gcggtgctgt tggagcccgt
    23881 gccggccagg acggcgacac gacccttggc ccgacgaacg acggccgcga tcaccgcgtc
    23941 attctcctcg tgggtcaggg tcgggctttc accggtcgtg ccacacggaa ccagtacctc
    24001 cgtgccgctg tcgagatgaa aatcgacgag tgcgtccagc gttttccaat cgacctcgcc
    24061 gttggcgtag ggggtgacca atgccaccat gctgcctttg aacatgcttt gactccgttg
    24121 cagtggaatc cttcccgccg gcctgcggtc aagcgccgag ggccggacgg gagctcccat
    24181 cgtcaaaaac agggcggtat tgtaagcctc tgcccggttt tgccaagtcc gggcaccccg
    24241 cagcggcaag gaggcgatca ggtggttgac tggcgggtgc aaccgtgcag gatatgcggt
    24301 cgtgaagcga atccgagtct gcccggtcaa gcggtcgccg ggcgttttgg ggccgttgta
    24361 tgagaaggga gcagcctaat ggagaaaccg tcaagcgaac gcaagcccga agtcaaccgt
    24421 cgcgattttc ttaagggggc ggccgcttcg gccggcgcgg cgatgggggt cgtggtcctg
    24481 gaaggttgtg ccggtctgac gcgggtgggt ccggccgcgg cgattcccgc gttcaagccg
    24541 cgggacgtcg tgcggatcgg ctttgtcggc gtgggaggca tggggacgaa ccactgcaat
    24601 aacctgttga agatcgaagg ggccgagttg cgtgcggtct gcgacatcgt gcccgagaag
    24661 gttgctcatg tacagagact ggccgagcag gcgggcaagc ccaagccggt cggttacagc
    24721 gccggccctt gggacttcaa acggatgtgc gaccgcgagg atctcgatct cgtgtacacc
    24781 gcgacgccct gggagtggca cgttcccgtt tgcctgtatg cgatgaagac cggcaaacac
    24841 gccgcgacgg aggttccggc ggccatgacg cttgaagaat gctggcagct ggtcgagacg
    24901 tccgagcaga cgggccgtca cgccatcatg atggagaact gctgctacga ccgatccgag
    24961 atgatggtgc tgaacatggt tcgcaagggc cttttcgggg agattgtcca tgcggccggc
    25021 ggttaccttc atgacctgcg ggcgctcaag ttcagcagcg ccggcgaggg tctttggcgg
    25081 cgggctcacg gctggagact tgacggcaac ctgtatccga cccatggtct tggtccgctg
    25141 gcctggtaca cgaacatcaa ccggggcgat cgttttgatt ttctggtctc gatgagcagt
    25201 gccgccaggg ggatgcaggc ttatgcggcc gagcatttcc cgccggatga tccgcgccgc
    25261 aaggagaaat cggtgctcgg cgacgtgaac accaccctca tgcgcacggt caacggcgtc
    25321 tcgatcatgc tgcagcacga caccaacctc ccccggccgt acagccggat caaccttgtt
    25381 caggggacga aaggcatctt ccgcgggttc ccggaccaga tctacgtcga gggccgcagc
    25441 aaggagcatg agtgggagtc gccggaggcc taccgcaagg agttcgccca cccgttgtgg
    25501 gtgacgatgg aggagaaagc caagggggcc ggccatggcg gcatggactt catcgaggac
    25561 tatcgcctga ttaaggctct cagacacgga tggtatcctg acctggatgt ctacgatgcc
    25621 gcgacctgga gcgcggttgt cgatctatcc gtcaaatctg tggccggccg gtcgagaccg
    25681 gtcgatttcc ccgatttcac acgcggcaag tggaagacga caccggccat acctatcttc
    25741 gatgcggacc agccgtgtcg tgcttgatgg cttgtcggcg gctgggcggc tggggaaaag
    25801 gggagttctt ggccagcatg ctcggcgggc gcagctcgag gaacggctgg ctggacatgc
    25861 ggatcagcgg tgcgggccaa aggccgatca gcaggacggc caggcagcag cagaacacac
    25921 cgaactggag cggtcggctg cgggtgacga gagcaaaccg gtatcgggga ggccgcagat
    25981 agcaggcggc gacgattcgc aggtaatacg cggcggcaat ggcggcgttg atgacgccaa
    26041 tgacggccaa ggcgatcagg gcaaggcgat gggggtggct cgggtcgact gacagggcgc
    26101 tgctgaagat gtacagcttg ccgaggaagc cggccgtggg aggcaggccc atcagactga
    26161 aaacacagac cgccagagcg aatgccgctg acggatggca ccgcgacagg ccggccaagt
    26221 cgtccaattc ctcgacttcg cgcccgtcag ctcgggtgac tgacagaacc gcgaaagccc
    26281 cgaggttcat caggccgtaa acggtgacgt aaaagagcat ggcggagagg ccgtttcgca
    26341 gcggtccgcc tcccgcgacg gggccggcca gcacggctac cagcatgtag ccggaatgag
    26401 cgacgctcga ataagcgagg atccgcttga cgttggtctg caacagggcc agggcattac
    26461 cgacgatcat tgttgccacc gccgcaatcc acaggaatgc aaagccgatg tcgggaatct
    26521 cccagccaag cgggccggtc cctcgcggct ggaccagcat gagcagcttg atcagcgcaa
    26581 cgaagccggc cagtttggga aagaagccga gcagcccggc ggtgggggag gccgagccct
    26641 gatagacgtc ggcggcatag gcgtggaagg gcacggctgc gatcttgaat gcgatgcccg
    26701 agaaggccag gagcataccg atgatgacgt aggcatggtt gccgtcgaga ttcatccggc
    26761 tcaggcaggt gtcgccggat gctccataga gaaagctcag gccgtaggcg agcagtgcgg
    26821 cggccaatgc gccgaggaag aaatacttga agcccgcttc ctgggcacgg atgtcacttc
    26881 gtcccatgga aaccaatatg taagtgggca cactaaccag ttcgacggcc aggaacagca
    26941 ggacgaggtc gtcggccatg gtcgtcagca tgatgccggt caggctgaac aggatcatgc
    27001 tgaagatttc gccgcgctgt tcctcctgcg ggatgtgatg gtgaaccaag agcacgacca
    27061 ggccgatgcc caaggccgtc aaacgcacat accaggtcag gctggtcagt tcgacgccga
    27121 atgcgcggat cggccgttcc agcgggccgg ctgcccatgc tattgccaag gcagccaaag
    27181 cggtgagaaa cgccaggaga aacgaactgg ctcgcacggc ttcttgtcgg gccaagccga
    27241 taagcaagac cagcgacgcg cctgccacga gtgtcacctc gggggccaga agaagaaggg
    27301 aggaatcatt catagtcgag gcctccgtgc catgaggctc gacgagtagt cccgagtggt
    27361 gaacgacggt tcaagaatgt ttcatccggt agttcgtggc gctggacggt gattcctgaa
    27421 tcctgggttt tcccaggcgt ctctcgcagc cgggccagaa tctgtctgtc tgccgccgga
    27481 cgaatgctgc tgagaatcac attgggaaag aggcccagga cgaggcatgc ggcggcaatg
    27541 ggggccagaa tgccgatttc gcgtttgtcc aaatcacggg acaggccggc gtagtcttcg
    27601 ggcgcccgtg ccggctcgcg caaggggccg aacagcaccc gccggcacag atacaacatg
    27661 taagtcgctc cgaggattac acccgaggcg gcaaaagcgg cgtgcatgat tcctagcggg
    27721 ccggtgccat cggcgttcga cgtcagggtg ccgagcagga cgaggaactc ggccacgaag
    27781 ccgttaagac ccggcaggcc gatgctgctg agagtgaaga agacaagaaa gaacgccatc
    27841 cagggcatgg gtcgagccag tccgccaagc tggtcgaatc ggcgggtgtg ataccgctcg
    27901 tagatcatgc cgacgacaag aaacagggct ccagtggaga ggccgtggtt cagcatgtac
    27961 atcaccgagc cgacaagccc tgcggatttc aggctgaaca tgccgagaat gcagaagccg
    28021 agatgcgaga cagaggaata ggcgatgagc ttcttgatat cctgctgaac ccacgccgcc
    28081 agggctcccc agatgattcc gccgatggcc agtatggcca tcagaggagc aaacgcaaat
    28141 gttgccgcgg gaagcatcgg caggctgaac cgcaggaatc cgtaggtgcc gagtttgagc
    28201 aggatggcgg ccagcaggac actgccggcc gtgggtgctt cagtgtgggc caatggcaac
    28261 caggtgtgga gggggaacag cggcactttg atggcaaagc cggctgcaaa ggcgacgaag
    28321 agccaccatt gcgtctcggg cggcagccgc agggcataga gtgtctcaag gtcgaaagtg
    28381 aacacaccga cgccgtttgc cgaagccggc agcgaatacg cctggtaagc caggaagaga
    28441 acgcctgcaa aggttaagac actgccggcg aaggtataga tgaagaactt atgagcggcc
    28501 tgttgccgtt tcggaccgcc ccagatgccg atgatgaagt acagcgggat cagcgtgaac
    28561 tcgaagaaga tgtagaagag cagcagatct cgggcgcaga acacgccgat catgccggct
    28621 tcgagcagga gcatcaggac atggtattcc ttgactcgcc ggcggatcga cgcgaagccc
    28681 gaccatagtg tcagcggggc gatgccggcc gtcagcacaa ccagccatag gctgataccg
    28741 tctacgccga catgatagcg gaaatcgacg tagccggccc cgcgagctgc cgatggggcg
    28801 gtctcttcgt cgctgaccca cagccggtcg agacacagcg catagcgtcc ctgctcggca
    28861 agagatcgac cactgcggtc tactgccccg aagaatagca ggattgccag gagggtcaat
    28921 atcgcggtgg ccaatgagaa gcccattgca gtgcggcgga tccggcgggc ggcggcctca
    28981 tccgcttcgc gagcctcggc gaggccaacg acaaccgcac ccaccagtgg cagggtgatc
    29041 agcagtgtca agatccaggt ttccgccaac tcagtcataa ggtttctact tcatcagcca
    29101 gacgatcaat gccatcagga cgatgccgcc ggtcatcacc acgccgtaac cttgcgtcgc
    29161 tccggtttgc agtgtgcgaa gcgccgcacc gagggcctgc ggaacggcca cgatcaatcg
    29221 cagcgtgccg tcgatgaaaa actggtcgag ggcgtacagc gaagcgccca acctccacag
    29281 gggccggacg atcagggcat cgtacgcctc gtcaacgtag tacttgttaa ccaaaagccc
    29341 gtggattcgg ggaaacgcct gacgaacggt ctgcgaccat gtcggacgcc ggacgaaaag
    29401 cacgtaggcg gccgctatgc ccaggatcgc cagtgctgct gacacataca tcagtgcata
    29461 tccgtcgccg tgagtccggt gtcccaagcg gggaatcgat gcttccttga acggtgcgac
    29521 gaccggggcg agaaactcgt ggaatgcgcc gctcggctcg aacagtcctg caaacccccc
    29581 tgggcgaatc gccacgcccg cgtagccggc ggtgacggtc cccgcggcca gcaggacaag
    29641 cggcaccagc atccagcggc cggtctcgtg tggatgaacg ccttcgggaa ttcgcggttc
    29701 gccgtgaaag gcaatgaaca tcatgcggaa ggtgtacagc gccgtcagga tggccgtcgt
    29761 catgcctaga attccataca ccggatgaaa cacgaaggcg gcgtgaatga tctcgtcttt
    29821 gctgaagaac ccggacagca gggggaagcc cgccaaggcg agcgagccaa tcaagaatgt
    29881 caggaaggtg acgggcatga tgcttttcag gccgcggaac tcgcggatgt cgatgatgcc
    29941 gcccatggcg tgcatgacgc tgccggcccc caggaacagc agggccttga agaaggcgtg
    30001 agtgaacagg tggaagatgg ccgagtccgc cgcgaaaacg ccgacgccga ggaacatgta
    30061 acccaactgg ctgatggtcg agtaagccag gatccgcttc atgtcgtact gggcgattgc
    30121 gatggttgcg gcaaataggg cggtggccgc cccgacaacc gcaaccgtgt tcaatgcgac
    30181 cggcgaagcc gcgaaaacgg gaccgcatcg cacgaccatg tagacgccgg ccgtgaccat
    30241 cgtggcggcg tggattaatg cggagaccgg actgggacct tccatggcgt ccggcaacca
    30301 cacatgtaac ggcaattggg cgctcttgcc gaccgccccg caaaacagaa gcagggcgat
    30361 agtcgtggtc tggccggcgg tcagatgtcc gagatagctg aaggccgtgg cgtagtcgag
    30421 cgggctttcg ccgggcggca cgtgcggggc cagccagcgg tagagcagga gaacggccag
    30481 gccgaagccg aaatcgccga tgcggttgac cacaaaggcc ttcattgccg ctccagccgc
    30541 ggccgggcgc tgatagtaga agccgataag caggtagctg cacaaaccga ctgcttccca
    30601 gccgagatag agcagaagga agttgccggc caggaccagc gtgcacatgc tgcagacgaa
    30661 caggcccatg aaggcgaaga accgttcgta gccgcgctgg gggcgtccat gatgtcgcat
    30721 gtaatcgcgg gagtagatca cgaccaacag agcaacgccg cagatggtgg tcagcatgat
    30781 cgttgtcagt ggatcgacct gaaaactgat gttgaacgag acgccggccg ccggaacgaa
    30841 ccatgaatag aagatatagg ggtcatcata ggctcgaacc ggcagtgagg cgacctcgag
    30901 aagaaggaga atgctgcata ccaaggccgc accgacgccg ataatgatcg gccagtgact
    30961 ggcgcccttg atcgcgcgcg ggccggccag tcccgcgatc gcggttccca gcaagggcag
    31021 cagagggacc agtggggcga ggttcagaat gtcggtgtcg actgcggcca gcatcatcgt
    31081 cgtgttgtcc gttttgccgc ggccggcatt gcctggcgaa gcatgagccg ccaagccggc
    31141 cgctgcgagt ctatccccgc atttcctccc aggcgtgact gtccaaggtt gtccttcggc
    31201 ggaacagcag gacaaccaat ccgagggcca agctggcctc tacggccgcg accaccacca
    31261 ggaacaacga gaacacctgt ccggtcaaat ggccatgctt gaggccgaaa cccacgaggt
    31321 tgagaatcac tccctggaac atgacttccg tggacaggaa catgaggatc atgttgcggc
    31381 gggcgagaaa accggcgaca ccgatcccga agaggatcgc gccgaccaga atgtaagcgt
    31441 tcgtcaggtc catcgttttc ccggcgttgc agggccgacg cccctgtcgg cccatctttc
    31501 cgcgatgcat ccccgagccc aacgcagtgg ggccgacgcc cccatcggcc catctttccg
    31561 cgatgcatcc ccgagcccaa cgcagtgggg ccgacgcccc cgtcggccca tcccatttca
    31621 aaacggcggt gcttcacggc cgcgccgctt gatatccgtt tcttccgcct gcgccgccaa
    31681 ttcggcgggc gtcaaagcct cagcctcgat ccgcttgcga gccagactca cagctccgac
    31741 aagtgcgacg agcagcagga tgcccgcggt ttcaaccgcc aggacatagg tggtcatcat
    31801 gctcttgcca atcaaccgca cgttgccggc ggactcgtcg tcgacggggg acgactgccc
    31861 ggcgtttgtg gacgcggcaa gcgaccgtcc ttcgctctca gtggcaccga caagcacgcc
    31921 ggtgtttgcc atctgcgagg cggaatcagc cgaaaccatc gcctggacga cggcagcgga
    31981 cagaagaaag cccagcgtga ccgccgccag cggctcacgt cctgtcaggt tcgccggatc
    32041 ggtttcctgc tgctgcgtca gcatgatcac aaagacatag gttacaagaa tcgctccggc
    32101 gtacacgatg accagggccg cgccaaggaa ctcggccagg gccagcagac atagtgcggc
    32161 caccgccagg accacgagca cgaagtacag cgcgcagtag atcggcttcg gatgcgtcac
    32221 cacccggacc gccgccacaa tcgccaccag ggcgaagaga acaaagaacg ctcgtccatc
    32281 gaacgcctca ccgatccagc gaacacagta cgttgcgatt gccgccagcg atgcggcccc
    32341 gagaatgcgc gcggtccatc gcagcggtcg gcctgttgat cgaccggcgg gcatggacaa
    32401 atacacgccc gccgcgccga gggccagaaa ggcatacaga atgtacatgc cagtcgtggt
    32461 cacaagctac cggcctccgt ttcattgccg tatggtcggg gtgacggcga aatcaagccg
    32521 ccggtgccgc catagccggt gatgggcggg ttcttgcgcg gcttctcgtc ccgcgtctga
    32581 tcgaacactt cgagcagctt gttgatatcg aagatcatct cctgccgcga gtaccccacg
    32641 atcgagtatt ccggcgtcag ctcgatcgca tctaccgggc aggcctcttc gcacatgccg
    32701 cagtagatgc acaccagttc gtcgatgtcg aaacgtacgg ggtacttctc gcgatcgggc
    32761 cacggtgcct ccgccccgac gatatgaatg caatgagccg ggcaggccgt ctcgcacatg
    32821 aaacaggcca cgcaggccac gcggccctgc tcgtcgcggt tgagccggtg gacgccgcga
    32881 tagatctcct tgcggacctt tacccgctcc tccggatact gaaccgtcaa ccgcttggcg
    32941 aacagatgcc ggaacgtggt cttgaggccg ttgatgatct gcggcagata gagcttgtcc
    33001 gcggccgtca acttcggctc agtgatctcg atgatgtcct catccttgat catgcgcgat
    33061 cccgccgtca tgggatgaat gccaggtcca cctcgatcgg tttctggttc gtcttgccga
    33121 tagagaagtc tccgctcagc ggccgatccg agaatccgcc gagatcggct gttgtcaccg
    33181 tgtaatcgcc gaggaatccg cggaagcgga cctcgccgtt cccttccacg gtcgccacgg
    33241 tctcggtggt ccaccacgta cccttgatcc gcttcatcag ctcctcgtag gccggtttgg
    33301 gcgtcatgtc ttcgcgcagc agaccggcgg gggcaccttg ccatgcgtta tagtccgtga
    33361 agtcccacca ggtgatcgcc tcgacggccg gatggctgaa caagatggta tagaaccgca
    33421 cgacttcttc ggcttgtcgt ttttcgccct cgggtgtgct ggcccatttg aacttggagc
    33481 cggcctgctg ccgctgctcg gccagattcc tgccttgggc gcccgaaatg aacgtcgttt
    33541 cagtgaagtg caacggcttg ccgtagcggg caaacctttc gcagacgtcc cagatctttt
    33601 cggccggcca cgctccgccg tgctgatgag actgaatgcc gatcacgtcg taaaggggcc
    33661 tgccttcgtc gtccaccaac tcgtcgatca cctttgcggc gaactcgtcg ccgacggcgt
    33721 aatcattgat gattaatgtg gccttgggat tgcctttgcg cgcggcttgg aaggcggctc
    33781 ggacaaaggg cccgactccc atatcacgaa tgatcttggt cagtctggga gagttcttct
    33841 tgcagtccgg ccgatcgtag tgcgttgcct cgttgaccac gtcccagata tcgatctgcc
    33901 cggcgaagcg gcggctgcac tcctcgatcc gctcgagttg aagtcgcaac acctccttcg
    33961 gatcgtccgg aagccatttt ggatcgaacc agttccaggc cagcggatgt cccttcgtca
    34021 tcacgttgtg ggcggcgcac cagtcggcca tcaccttggt cttgtcataa tcaggccttc
    34081 cgcgatcttt ctcatagtgc caccagtaga aaggcaacgt cgcgtagttg agcagggcgg
    34141 cgtaatgctg ctcgtaagcg gcattggcct cggggctccc gcaggcgcca agcttgaaga
    34201 tattggagcc gaagagaaac gcatggcgcg tttgtctgat ccgcacgtga agcccggcag
    34261 gaggcactct gccattgttg gtgagtcgga gcacgatatc gcccttgcga tgcttctcaa
    34321 tccgtgcgtc cgcctggccg agaatgtcgg cctccgggag cggggcggcc agggttgcgg
    34381 ccgcaagaat cagtgtcaga acgttcattc gaacctcctt gtcagcgagc cttcgatggg
    34441 cggcaggttg gcctggcggc cggtgatggc cggcgaccag acatagaatc tcaacacggc
    34501 cgccccgaac acggctgcat tgcacagcgg cgaccagaac gacgcgacga cgttcttata
    34561 gagcagcaac gaggtcagga cgaccaacgc gatgcccgcc ggcaccaggc cgttccaggc
    34621 aaaccgcatg agctggtcga atcgcggccg aaagagcgtc catcgaaccc acataaagaa
    34681 aaggatgaag acgacgacct tgctgagcac gatggcaaac tggcacaagg cggcggcaag
    34741 cgtctgcgac ttgtagatcc agtcgagcca gccccagccg agccgcgtgc tgaaggggaa
    34801 cagctcccag ccgccgagaa acagagcggt catgaacgcg cttactgtga tcaggtgggc
    34861 gtattcgcca aggaagaaca aggccatttt catcgagctg tattcggtgt ggtagccccc
    34921 gacgagttcc tgctcggact cggccaggtc aaaaggcatg cggttggtct cggcgaaggc
    34981 cgtgacgagc agtatcagga acgccagcgg gtgcagcaga acgttccacg tggactgcat
    35041 ctggtactcg acgatcttct ccagccgaac ctgcccggtg gtgagcacca cgacgaggat
    35101 ggccagcccc atcgggatct cgtagctgag cgtctgggcg gcggcccgca ttccgccgta
    35161 aaaggagaac ttgttgttgc tcgaccaccc ggccagtacc agcccgtaca cgcccatggc
    35221 cccgaccgcc agaatgtaga gcaggccgat gtccacgccg gccacctgca cgcgcaccgg
    35281 ctcgggcccg cccaggtcaa gcgggccgcc ccaggggatt accgcgaagc cgatcattgc
    35341 aacggcaaaa gcgatgcacg gcgcaaggaa gaacaaaggc cgatccgcgc cgcggggtgt
    35401 gaaatcttcc ttgagcagga acttcacccc gtcggctatc ggctgcagca ggcccagcgg
    35461 cccggcccgg ttcggcccga ttcggtcctg gagccatgcg cataccttgc gctccagcag
    35521 gatagcatat gcgacgaacc ccatgatgat agtgatgagc accaccatca gtgccacgct
    35581 gaagccgagt tgggtcttga gcaagtcgat catcttcagt gctgaaatct cggctgcggt
    35641 tccggcacgt gcacgtccgc catggccggc accgtcggga tcatcatctt ccgcaccttc
    35701 tcactcgcat acaagcctgt gtggccggcg atcgcgtaca ggtactgccc gtcatgcatg
    35761 gctccttcgg gcggatcgac cgctctctca aacggttgta ccaggccggc gtggttcatg
    35821 aacgtcccct gacgctccgt ccacgcacag aagggcagca caacgtcggc ccgtgcggtc
    35881 agcctgttgg ggaacatgtc ctgaacgatg agcaactcgg gttttgcggc cgcgtccggc
    35941 aacgtctgtg tgggccagtc gttgtcgggg taaccgccga cgatccacag ggcctgaaaa
    36001 cgcccggcgg cagcctcttt ccataattcc tcacgtggca gcaccggccc gcccatcgcc
    36061 gttagaatcg cctcgatgcc tcggcggttg gggtgcttct cgggtcggat gacgaatctg
    36121 gccgactcct cggccgcccc gacgggaaaa acctccggct cgccaatcgt gtggacgtcg
    36181 cccagcgaca gggcggcgtg cggagcgatc cggcggacga agtcggccag gagccaagcc
    36241 tcttcgcagc acatttccgg agacaactgg acggcgaccg atgcggggcc gtgccggtcg
    36301 attgtttggc ttagtcgccg gcggacgagc tccggagccg attcccagag cagcggctcg
    36361 tggccatttt cgcaacgaac ggcgggcgtc gacagccgtt tctcatcgag aatgaacttc
    36421 cagccgaacc gcccgtcgtc acagatccac cagtcgttca cgccaacgtt ccatcgaggc
    36481 ctgagccggt aaacacggtt gcggttttga tcgatgcgga tcgcgcagcc ggtgctgcac
    36541 gcccggcaga tcgagttggt tgtcgtcagg aaccataccc gttgggcgaa gaggaaatcc
    36601 ttgtccagca gcgccccgac cggacagatg tccacgacgt tgccctgcag cttgttggtc
    36661 agtggtcggc cggggaaggc atcaatttca gcgacatgac cgcggttcac gatgcacaat
    36721 tcactcgtcc cggagacttc gcgagtaaat cgcacgcagc gagagcagag cacgcagcgg
    36781 tcctggtaga gcagggttcg cggtcccaca tccttcttgg ggttgacggt cttggggtcg
    36841 accattcgcg aaacggcctc gccgaacttg aggctgtaat cctggaggta gcattcgccc
    36901 gcctgatcgc agaccggaca gtccagggga tgattcaaca ggtaatactc catgatgcgc
    36961 ttgcggtgct cgtggatctt ctccgtctcg aaacgaacct ccagcccgtc gcggaccggc
    37021 gtctggcaac tgggaatcag ccgcggcgac cattcgaggt ccccgctctt ggggtcgagt
    37081 atcttcattt ccatcaaaca cagccggcat gaggcgacga cagacaggcc cgggtgatag
    37141 cagtaatggg gcacatcgat gccggcatcc agagctgcct ggagcacggt aatgccgtcg
    37201 cggcattgaa ttgttcggcc atcgatgatg attgtgggca cggatcagtc tattagcctg
    37261 tcagtctgtt agactgttag gaaacgaaca gaacctggtc gggttgccgc cccggcagat
    37321 cgaacatgct cacttcctag tagtctagag gtctagcagc ctagtagcct cctcatcctt
    37381 ttctccccgc gatcggcatc tcgtacaccg cgggattgat ccttcgcaac gtctgcgcca
    37441 ccgccgccgg ttcctggcgt cgaatgtgct cctcgaactc gcttcgatac ttctcgacga
    37501 tcgttctgat cggatacacc gccccgtcgg gaagaccgca gatgctcatg cccggtatca
    37561 tgcccatgtt gtgcgaattc tcgatcagga tgtcgagatc ctcgctgcgt cccgcgccgg
    37621 cggcgatgcg cttggcgatc ttatacatcc aggcggatcc ctcgcggcac tgcgtacact
    37681 ggccgcagga ttcatgggcg tagaaccggg ttacattcag cagcaccttg cggatgtcgg
    37741 tttcctcgtt gatgacgatg gccccggccg tgcccaggcc gagcaggccg tatttcaccg
    37801 gatcttcgaa gtccagcttg cagtccaatt catcgccggt caggatcccg accgacagcc
    37861 cgccggggat cacgcccttg accttcttgt cgccgatcat gccacggccg aaatcgtcgc
    37921 cgtagatcag ctctcgcacc gtcagcccca gcggcgcctc gaaacagcct gggcggttga
    37981 ccggcccgga gatcgtgtac agcttgggac cgttgcttcc aggtgtaccg atggagcgga
    38041 accaggccgc gccttgctcg atgatgtgcg gaaggcaaca gagcgtctcg acgttattga
    38101 ccaccgtggg ttgtccgaaa aggcccttga tggcgggaaa gggcggcttg attcgcggcc
    38161 agccgcgctt tccttcaagc gattcgatca gccctgtctc ctcgccgcag acgtacgccc
    38221 ctgcgccgcg atgaatgtgg caatcgagcg agtaatcgca gccgaggata cgcttgccga
    38281 acaggcctgc cgcgtaggcc tcgtcgaggg ccttctgaag gatgtggaag gtctcgtgga
    38341 actcgacgcg gatgtagata tatgcgacgg ttgcccgtgt ggcgtatcct gcgatcagga
    38401 tggcttcgag caactggtgc gggtcatggt cgacgatgta tcggttgttg aacgtgcccg
    38461 gttcggattc atcgaagttg acgcagagat atgtgatgct cctgtcggcg ggaaggaacg
    38521 accatttcac gccggtcggg aagcctgcgc cgcctcgacc gcgaagattc gatcgcttga
    38581 cttcgtcgac gacctgctgg ggagtcatcg tgcggagcgc tttggccgcc gcggcgtatc
    38641 cgccccgcgc gcggtacgtt tccagccgcc gcacccctgc gacgccgaca ttcctcaaca
    38701 agaccggttc aaaccgtttc atgatcactg cgctgtcgtc aggcgcacct cacgccctct
    38761 tgccgaaccg tgcttgccgc gccgtagcgc accgaaggcc ggaacggtgc cttctcacgc
    38821 cttcaccccg tcatacaaat cactccgttc caagccgatc ttgtcattgt cggggtctgc
    38881 cagaatgcgg ggcacgtcct cggggcgcac gtttttgtgt aaccgctcgt tgaccagcag
    38941 gcagggggcg tattcgcacg cgccgaggca ttcctccgtc acgaaactgt attcaccatc
    39001 tgcggtggtc tcgtgctccc tgatacccag gtgctcgcaa accgcccgat tgacctcctg
    39061 gacgcccatc agctcgcacg agagaccccg acaggcgacg atcactttgc gtcctctcgg
    39121 tttggtccaa tagtgcgtgt aaaaggatgc cgtgtccatg acctctgatg gagcaagctc
    39181 cagcagctcg gccaagtcgc gcatcgcccg gcggctcacg cagccataag tctcctgcac
    39241 gatgtgcagg gccggcagca gggcggcccg cttgctggga taccgggtga agaatccgcg
    39301 gattctcgcc ttcaccgcct cgctgatctg cggagattcc gcgtcgtccg gtgccggttg
    39361 attcctgtcg atcgttcgcc acgccatcgt tttctctgtc gttcttcgcg gatcaccggg
    39421 ccgacgctca tgtcagtccg tttgactccg atcgtggggc cgacgccctc gtcggccctt
    39481 tgtgactctg accgtggggc cgacgccctc gtcggccctt tgtgactctg accgtggggc
    39541 cgacgccctc gtcggccctt tgtgactctg accgtgaggc cgacgccctc gtcggccctt
    39601 gctgactcac ggtgttcgtc gaagtccgcc gcacggcccc gggcttgccc gtggggttcc
    39661 attggcgatc ttcatccgtt acctgtccaa ctctgcggcg atgatgttca ggctacccaa
    39721 caccgcgact acgtcgctga tctgatgccc gacgatcagg tgttcgaagg cctgaaaatg
    39781 aacaaggctc ggcggccggc agcgggcgcg gtacgcacag cgcccgccgt cgctcaccag
    39841 gtaaaagccc agctctccgt tggccgtctc attggccccg tatacctcgc ccaccggggg
    39901 cgtaaaccct cggttggtca tgatcagctc gaagtggtga atcaatccct cgatgctgaa
    39961 gtagacctct cgtttgtcgg gcagtgtgac cttatcgttt ggaagcacat tcaccggccc
    40021 gggcggaatg ttgtcgatca actggtggat gatgcctgcg gcctgcttga tctcctcgat
    40081 cctgaccagg taacgagcga gcacgtcacc gccggtagcg atgggcacct ggaacgagac
    40141 cccggacgag cctttgccgt cccaattgtc cgcatagcag agatacggtt catccttgcg
    40201 gagatcgcga cgcacgccgg aagctcgggc cagcggaccc gtccagcccc aggagatggc
    40261 atcgtcccgg ctcatatagc cgatgccctt ggttcgctcg ataaacagcc ggttgcggtt
    40321 cagcaggttt tcgagatcgc tgatcgcccg tggaaggcgt gcgaggaact ccttcacgtg
    40381 ccggggccat tcgggcggaa tatcgaacat gaggccgccc acgcgcgtgt aactggtcgt
    40441 gaagcgggcg ccgcagacct cttcgaaaag gtcgttgatc cattcccgct cattgaagcc
    40501 gtagagaaag gccgtgaatg cgccaaggtc caaggcggcg gcccccacac aaagcaggtg
    40561 gtcctggatg cgcgccaact cggcgatgat cgttcgaatg gccttgcagc ggggtgtgat
    40621 ctcgatgccg aacaacttct cgcaggtgtg gtgccatgcg atgttgttgg ccatcggact
    40681 gatgtagttc atgcggtccg tgattgtcac gtactggttg tagtcgagat gctcgccgag
    40741 cttttcgaac ccgctatgga gatagccgat atgcggggtg caggctacta ccgtctcacc
    40801 gtccagttcc aggacaagcc gaagcgtggt atgcgtggcc gggtgctggg ggccgaagtt
    40861 caggcaccaa agctcgcgat ggccgggacc ttcgagcagc ccctgtcgca cgaaagtcgt
    40921 tggctgtggc gaacttacgg acactcaaca cgcccctgtc gtcaccatgg cgcccggttc
    40981 tttcggcacc cgtcgcgtcc agcctggtta ctggctcggc ggagcgccct ttgtgcccgg
    41041 cgtcacgaag atgatagggt cttggcgcgg gggcggcaag gcgggggcag gaccgcgcga
    41101 tgttgttttg agttgtgcgg attccgagcc tatagtgact tgcgattcaa aggggtggga
    41161 cattgcttgt gcgggaacgt cgatcccagg acggcaaggg acgtggtatt gaagagcagc
    41221 ctacaactcg tgccgatgca cgtcgggcaa tgcacgctcg gcaagaacca cgtcctgggc
    41281 gatcctcata gcgacgagga tcgggttgat ttcacgcttt atgcgttcct ggcggatggc
    41341 ggtcccgggc gtcgtgcctt gatcgatctg gggcctgtcg gactgcccta catcaactgg
    41401 atgttcaggc gctacggctt cttccgcgat ttgccgggcg cccccgacga tgtgcgtcag
    41461 ccgcacggca acatcttcga ttggctgagt cggctgaaca tcgctcccga gagtgtcggc
    41521 cacattgtct ttactcattt gcacgctgat caccatggcc tgaccgacgg caaggatggc
    41581 ggggcgatcc tgcgattccc caacgcccgc atttacgttt ccaggacagg ctgggaggac
    41641 aacctcacaa aacgggtgaa cggaggctgg aacagttacg tcgatttcgc gttctccgac
    41701 ttcttgcttg acgccgacaa ggccggccgg ctggcggtcc ttgatgaggg cgaggtcatg
    41761 ccgggcgtaa cggtgagcta tcttgggggg cattcggtgt gcagcctggc cgtgcgcatc
    41821 gagactccct cgggcgcggc ggtggtctgc agcgatgacg tctatcggta tgacctgctc
    41881 gagcagggta ttctggcgcg gctgcgggtg actcacgccc ggctgctcgc ggcgaccgaa
    41941 cggctggtcg gtatggccga ggccggagcg attctgctgc cgtgtcacga tccaggcatc
    42001 ttcgatatgt acagccgcta caagcaagaa tggctggggc atctcaagcc tgtgtcgcac
    42061 cgcgctgccg ccgggttccg acgttccgcc aaggtcctgc tgggccggtt gccggatgac
    42121 agcgtgccct ccccagcgcg gccgcgagac tgcaagacac cggctatgcc ctgacttcag
    42181 acaagccggg cctgctgcag aatcaaatgg cgtgccggta cctgaagcgg gctttgcagc
    42241 cctcttctcc aaacggtttc ttcttgaggt gcggaagacg tcgtcgaaga aggggagtct
    42301 gacgggtggg tgttgatgtc aataagcctt catccgcgat gcttttctta agcggcctgc
    42361 cttaggaatg ccggccacgt ttcaactttt gcttaccttt gccggtgatg ctggttgagg
    42421 attgggcaaa gagcttccgc cctgccatcc gaacggtttt tcctcgccgc tcaattcgcc
    42481 agttggattt cacagatcag cggccgatgg tcagacttcc acgtccagcc caactcgcaa
    42541 cgtgtcaccc gaagcagccg actgacccat gcttggtcga tggccagcac cgggcatggt
    42601 atcggccacg ttgcgtaatg gcctttgccg gctgcctcga aggcattaag gaaatcctgc
    42661 cgaagtccgc gaaggtggat cgaatctgca ggcgtattga aatccccgat gatgatcgtc
    42721 ggctcatttg acatcgagca agccaagcga gtcaaggtct gtatcgccgc cgcccgcgag
    42781 taaaacgggt tgctcctgat ctccggttgg atgacgctga gacgaacacc gccgaccagc
    42841 accgacgtgt gtttgtacca ggcgcggtct gccaaatcgc cacattcatg ctcgacaagc
    42901 tcgccgcgcg tgatcagtat cataccatga tagattgtcg agatgttgta tccggggaac
    42961 gccgccgcgt attgggcgat tcgttccttg gtggccgggc cggcctcatt catggcgatg
    43021 atatccggat tgacccgacg gatctgatcg atgacgcccg gccgttccaa ggcgcgccct
    43081 gcggtgttcc aggccaggac ccgcacgcct ttgccgtcgc tggccgtcgc ggaagtgaaa
    43141 cgggtcgcac agtgccacca tgccatgcaa gccacggcgg caccgaggca caaccagccc
    43201 atacgccgac gacgccggac caggagcaac aagcaggcta ttgcggcgat gcctgccagg
    43261 acaatgatgg gcgttgcgta gaagatggtg gatgccggaa caaagctatc ccggacggtc
    43321 agcctgatca gcagagcgac aacccaataa accgcgagga cggcagcggc ttggtcagcg
    43381 atgctcccgg cgtttcttcg gcaccacgcg atcatctgac gttcccgacg gggcctcgtt
    43441 gcccgttgcg gcccaaacag acggggcatt ccgcacctgc tgcttgcgga atgccccggt
    43501 tctggcgaag aaagcaggcc gacccttacc tgccgagcag gtgctcgatg accatgcgct
    43561 ccagtccttc gtaatcgcgg ttgcggatga actccgcctc ttttttgcgg ttgaaggttc
    43621 tcaccttggc cagcagcagc tcgaagaatc gcttgctgtt ggccaggtgg gcggtattat
    43681 tggactgttt ctgtgtccgc atgactttga cgtccagacc gaccatctcg ccgttgaggc
    43741 cgaagccgcc ttcctcgagc actcggacct ggttgaacgc tcgccgcagg ttgtcggcgg
    43801 caaagctctt gtcctcgtca aacttcaagc cgttctggtc gttcagatgc acgctccaga
    43861 gcttcttgtg gtatagggcg aaggccatct cgtccgacgg gtccagcccg gccagcaggg
    43921 cgtgggccgt ctcgatgagc ccgccgaccc gcgacgggtc atcggagaga taggccagcg
    43981 aaatggcgtg cccgatcgtc ggtacgtagg cgtggtccat gggctcgttg ggcttcggct
    44041 cgatcaagat gcgcacttcc ttgttgtggt tgagcatctt gttgatcgcc tgcatgaggt
    44101 actcgtgggc gatccgggcg ttcttagatt cgcggatgta ggtgccttcg cgggccagcc
    44161 agagaacgat ctggttggcg cccagctcga aggcgatgtc gatcgcctgc aacgagcgat
    44221 cgatagcgta tcggcgacaa gccgcgctgt tggaggtgta gccgccgtca atggtcatcg
    44281 ggttttccca caggcggggg gccacgaact cggggaccag cccgtggtcc ttcaggatct
    44341 tgccgagctg ggctgcacgc ttgagcacgg tcgaggccgg cagggtgtcg agctccggca
    44401 cggcgtcgtc gtcgtggaac tggaccccgt caaagcccag atccttgtag accttcagtt
    44461 tcttggcgaa gctgaccggt tgccggacgg gagggccaaa ggggtcggcg ccttcatgaa
    44521 tgttccaggg tccgaacgag aaacggtatc tcttggtctg ctttttagcc acgctacgtt
    44581 cctttcgagt tcaaggctcc aatacgaatg catcgcaaca tgcccgggcg gcaagcccgg
    44641 aacacgctat tgccgatggt agggtacccc aagggtcatt ccccggcaag gcggctgcca
    44701 aggaggagcg agggggttcg tgacgcttca ggcgggaggg tgccagggct ctttcagaac
    44761 cgcgaccgtg agggagcggc gcagcacggg tcgccttcgc ttcgcggaac ctatgcttcc
    44821 ccgtgccagc ctgtgttatg gaagacaccg gtgaacaagc cgctgacggg acccgccggg
    44881 tgccatgccc acggctctgc gtgggcatgc ttgagctgaa agctgacagc agaatgctga
    44941 aagctcgcct ctctgaaatc tccattccca aatccgcaat cctgcccctt tgaatggtac
    45001 caacgcgtgg tgtcatgtcc acagctctgc gcggggacgt ggctcgatct gaaagctcaa
    45061 aaccaaaagg cgaatgctga tcgctttttt ctgtatagaa gtcgacaggt tcgagggtct
    45121 gcgcgccggc tcgtgatgat cccacaatct gacgagccgc gcggtgggcc cggacgcccg
    45181 agaggccggc ttcctcgcct aaccgatcac ggaccactgt ccactcgccg tc
//
LOCUS       DAOTPW010000009        42216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1012_length_42216_cov_5.304144, whole genome shotgun sequence.
ACCESSION   DAOTPW010000009 DAOTPW010000000
VERSION     DAOTPW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 42216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42216
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1012_length_42216_cov_5.304144"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="P5316_01470"
     CDS             complement(<1..229)
                     /locus_tag="P5316_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83653.1"
                     /translation="MPEPNDHSPDHLERRCRLLGHEIRFGYCRMLPEGRPCRLIVDCW
                     RGSFDVISYLKQHYTDEQIEAFLAPPKPKLTS"
     gene            complement(447..1835)
                     /locus_tag="P5316_01475"
     CDS             complement(447..1835)
                     /locus_tag="P5316_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRR83654.1"
                     /translation="MNTKVLLDESEIPRQWYNLAADLPNPLQPPLGPDGKPISPDMLA
                     PVFPMNLIEQEVSQERWINIPDEILEILYLWRPSPLYRARRLEQALGTPARIYYKNEG
                     VSPAGSHKPNTAVPQAWYNKQFGIKRLTTETGAGQWGSALAFACSLLGLECKVYMVKV
                     SFEQKPFRRLMMETWGGKCVASPSNETKAGRDILAQDPNCPGSLGIAISEAIEAAVTD
                     PSGKTRYSLGSVLNHVMLHQTIIGLEAKKQLAKIGEKKVDTVIACAGGGSNFAGIAFP
                     FVCDKIAGANIEVIPVEPSSCPTMTRGPYAYDHGDTARMTPLLPMHTLGHTFVPPSIH
                     AGGLRYHGIAPLVSQAIIEGLITPRAYHQLECYEAAVLWARTEGFISAPETSHAIACA
                     IDEARKAKEEGKEKVILMNWSGHGLMDLLGYDKYFRGQLEDFPLPEDEMQRSLAAIKD
                     LPKPKVMKSGKW"
     gene            complement(2301..3332)
                     /gene="argC"
                     /locus_tag="P5316_01480"
     CDS             complement(2301..3332)
                     /gene="argC"
                     /locus_tag="P5316_01480"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRR83655.1"
                     /translation="MPNQKIRVAVIGATAYTSREAIRWLLRHPQVEIAALCGRKDWMW
                     RPHIHEVFPEFVGRIELPVEEIEPASLAGRADVVMLCLPHAVSTQFVPTLLQAGLRVI
                     DFSADYRLKEPADYEQWYNKKHTDLENLAHAVYGLPEFYREKIRDARLIANPGCYPTS
                     AALGVIPLLKAGLIDPHEVIVDSASGMSGAGREPKPEHHFPERNENFEAYGVGTHRHM
                     VEIERTLDAYVRNGKSSVIFTPHLLPIERGILSTIYLKPLQPVSPEHVNQVFAEAYAG
                     EPFVRLRKDLPRTSLVTHTNFCDISARVVKSRIIVISAIDNLIKGAAGQAIQNMNIMF
                     GLDERAGLL"
     gene            complement(3598..4056)
                     /gene="rpsI"
                     /locus_tag="P5316_01485"
     CDS             complement(3598..4056)
                     /gene="rpsI"
                     /locus_tag="P5316_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRR83656.1"
                     /translation="MSEEVKEKATPTAGSATIPEPDPKKKYFWGTGRRKKAVARVRIR
                     PGTGKFEVNKRPMEQYFKEERDRQDCLAPLRAADLLGKVDVFVNTSGGGPTGQAGAVV
                     LGIARAIKVANSEFDAVLRGGGYLTRDSRMKERKKYGQRGARRRFQFSKR"
     gene            complement(4127..4564)
                     /gene="rplM"
                     /locus_tag="P5316_01490"
     CDS             complement(4127..4564)
                     /gene="rplM"
                     /locus_tag="P5316_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRR83657.1"
                     /translation="MKSYVAKPGDAGGQWHLVDADGKVLGRLAAELATILMGKHKPTY
                     TPHVLSGDFVVVINAERVKLTGRKLEQETYDYYTYYPGGHKIVPIAKLMKAHPDRVLR
                     LAVRRMLPKNKLAAGMLKRLKIYRGSEHPHAAQQPRPLELTRV"
     gene            complement(5069..5413)
                     /gene="metG"
                     /locus_tag="P5316_01495"
     CDS             complement(5069..5413)
                     /gene="metG"
                     /locus_tag="P5316_01495"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRR83658.1"
                     /translation="MSDQASVIQFEDFAKIDLRVAKVLEASNHPNADKLVVLKIDLGY
                     EQRQICAGIRGHYDPASLVGRNIVVVANLAPRVMRGVESKGMLLAASTPDHTRIILLQ
                     PDADIEPGSKVT"
     gene            5584..7254
                     /locus_tag="P5316_01500"
     CDS             5584..7254
                     /locus_tag="P5316_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HRR83659.1"
                     /translation="MFRIWLASVAVLSGWLAGASNAPASGPATTPGSPAAAQVRYCDV
                     TRFGAVGDGKTKNTQPIQETINAANEAGGGIVLIPPGIYLTGTIHLRNHVTLHLLPGA
                     TLLGSTDLADYPSTQVTFRSYTDHYVCQSLIAGENLHDIAITGKGIIKGQGADRAFSA
                     SAPDWGFRKRPYIIRLVSCRNVLIEDVTLRDSPMWVQHYLACDNLTIRGVTVRSRANN
                     NNDGLDIDCCRNVRVTGCDISSGDDALCLKSTADRSCENIVISDCVLSSACNGFKLGT
                     ETNGGFKNVTLTNCAMYDVNLAGIALLIVDGGTLDGVTVSNVTMRNAGSPIFLRLGNR
                     ARPFKDNMPSPGVGRFRNVAISNIVATGCERTGSSIVGLPDHLIENVTLSNVNLTYAG
                     GVTVAQIPAEIPEQPAGYPEHNMFGALPAYGFYCRHVDGLTLRDVAVRFAKPDQRPAL
                     VFDDVRNLAVSGLRAQGTGGTRATIAMKAVSRALISECVAPADAPVFTWIDEGCERIS
                     VIGNDFSETARPFVFAGSSLVGCLFESANRTGQTPVAPAASRTAPAEE"
     gene            complement(7411..9099)
                     /locus_tag="P5316_01505"
     CDS             complement(7411..9099)
                     /locus_tag="P5316_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRR83660.1"
                     /translation="MIRMRVHPTPGRSRFLAGTLAVFLLTVPAAYGADFHRLSVRDYR
                     DKMKAGWIGQIAGVSWGAPTEFRFADRIIPEDQMPVWKPEMINNAFGQDDLYVEMTFL
                     RSMEQYGLDVSIRQAGIDFANSKYPLWCANNAGRNNLRDGIAPPDSSHPKFNKCPNDI
                     DYQIEADYSGLIAPGLPNVAIKLGEKFGRLMNYGDGVYGGQFMGCMYAEAFFEDDPVK
                     IVEAGLKCIPEKSQYAEMVRDMLKWYREDPTDWEKAWHKAQKKYREDPEYQKASNGGI
                     DVKINGAYVLMGLLFGNRDLDKTIIISCRSGQDSDCNPSSSAGVLFTTIGFSKLPDRF
                     TKELDQTKVFSHTAYNFPALLEVCEKLARQALVQAGGRVEKDASGEEFFVIPVQAPKP
                     SPLELSWEPGPIAGSMFSADEMARITALGPKRAFEKFAPGWKIRDCGREMNPGLHDEV
                     RGRKNVYVSHPLNKKTGCTLYRSVDIPANKKTTLRLVVGHHEEGDWLLVVKANGETLI
                     ERLVGKESTTEGWMTIKVDLSKFGGKSAELELVNQPDDWQHEGAYWAEIALDSQ"
     gene            9286..10650
                     /locus_tag="P5316_01510"
     CDS             9286..10650
                     /locus_tag="P5316_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83661.1"
                     /translation="MRGPGEQMELSHHNLVNFAHLDHLCEEVVCNGEAYRIVHIYCPA
                     PDYNRTADPEEGTACVDDAARAAVVYLRHFEITGDEQSRIKAEGLLRFILYMQTPEGL
                     FYNFVHTNRLDINTTHERSRADGVTWWMVRAIWALGTGVRALWKANPTIAAQCEESLR
                     RCLPALSRLLERYPECENHRGRVIPTWLVNNDGADATSELLLGLTAYHQARPSSEIQT
                     MISRFAQGISMMRYGSMNQSPYGMHASWRDGWHLWGNSQTQALAEAGILTSAKMEAEH
                     FYPRLLVEGYLHSFTFDDLHNIRYFERIAYGVRAVAVGLVRLFEATGDERYAIMAGLA
                     GSWFLGNNLAGKPMYDRATGRGYDGLHQEGRINYNAGAESTTEALYTLLEVGNHPEAA
                     CWLNVRRQEKQRETREGVDYFYRVFEDATNHPPRRIGLILNLTHETFELMQGDALDRF
                     LGSA"
     gene            complement(10822..12177)
                     /gene="der"
                     /locus_tag="P5316_01515"
     CDS             complement(10822..12177)
                     /gene="der"
                     /locus_tag="P5316_01515"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRR83662.1"
                     /translation="MPLPMVAIVGRPNVGKSSLLNVLAGRRISIVDPTAGVTRDRVQA
                     LCELEGIYFDLVDTGGYGVVDRDDLGEQVEQQIRYAVDQATLILFVVDAQEGVNPLDQ
                     SVADWLRRCSKPVILLANKVDSPAARTELADLVRLGFGEPMPVSAVHHLGLSELKTRI
                     AEIVRPFSDGAAPPDPTMKIAIVGRRNVGKSSFINALAGQERVIVSQVPGTTRDAVDV
                     RFERDGHVYVAIDTAGVRKRSKIADDIEYYGYHRAQLSIRRADVVLLMIDATEDVSHV
                     DKTLGGYIVEQFKPCILVVNKWDLAKGRAVTGDYEEYLYKTLPGLDFAPIVFTTATQN
                     RNVQAVIDVAASLFKQARTRIPTGQLNQVVNEAMNANPPKPKRGVGPVKVFYATQVAV
                     CPPTIAFFVNDPGRVNPGFERFLLNRLREQLLFEEVPIRLLFRSRRPKPPPGDSPHGS
                     A"
     gene            complement(12245..13378)
                     /locus_tag="P5316_01520"
     CDS             complement(12245..13378)
                     /locus_tag="P5316_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83663.1"
                     /translation="MNSTMGIRGFGRGVLTGCLVFACAAAATGQEQQLNLADIRKNNF
                     GTIDRQRVEDWCRQRIRLIMASKEPLKDAPAFISEITGHIQAADATSAFKAGMVESMA
                     TAFAALYKPATDGKDPVALGNVVLLAFLRRHDSPAATACYKLAINDAFSGMRLVAAEG
                     LLKSKLSPQDWEELLPALQKQASIETDPATMDRLYRVLTMNGGPAVDRVVPVLSAILN
                     ARLTRFEQKGEFPAVADANVTIWLAGKFAQINNEQMKNDIVRGTARLLADAVYHYTKT
                     QAGREKREQLERVVLITEAQLKAMVRPSGGQTQPDVTSAMTEGGTERNAKMEKAMDAW
                     IGTAEVAGILNGAPYNFQRGLGIQRPAPTSGPASAPAATAPVR"
     gene            13707..14414
                     /locus_tag="P5316_01525"
     CDS             13707..14414
                     /locus_tag="P5316_01525"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRR83664.1"
                     /translation="MASDASAAGSRHDPQDHPAWDGIPAPAVRRLCLYLRELEGLLAR
                     GRLTVSSKDLGRTLGYTDAQVRKDLAYFGHFGQPGIGYGVEELIARIRGILGTDRIWN
                     VVVVGAGNIGQALSTYRGFLKRGFRIVGVFDNNPAKVGQRIGDPPGVEILPLDRLPEV
                     VASQEVRLGIVAVPAEAAQEVAEALLAAGVKGILNFAPASLAVPEPVPVVPVELSLQL
                     EQLAFQIRARSTRARAR"
     gene            14475..14747
                     /locus_tag="P5316_01530"
     CDS             14475..14747
                     /locus_tag="P5316_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRR83665.1"
                     /translation="MSRQDDSIDDPQDIDLEEGGAPDFEDCPNCGKSIPESVGRCPYC
                     GQWIACPSCGEWLTGASTAAGRARGWFWPVVVAILVAIILVMWTGL"
     gene            14779..15759
                     /gene="hemB"
                     /locus_tag="P5316_01535"
     CDS             14779..15759
                     /gene="hemB"
                     /locus_tag="P5316_01535"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRR83666.1"
                     /translation="MAFPIHRLRRLRMNPGLRRMVCETRLSLDRLIYPLFVRPGESIR
                     NQITSMPGHFQFSVDTAVEECRRIADLGIPAVLLFGIPSHKDAVGSEAWHDNGVVQRA
                     IRQIKKACPDLVIITDVCLCEYTDHGHCGAIVERAGRKDVDNDATLPLLVKEALSHAA
                     AGADVVAPSDMMDGRVGAIRRALDEAGFAHLPIMAYAAKYASSYYGPFRDAAESPPRF
                     GDRKSYQMNPANGDEALREVAADIEEGADIVMVKPAVNYLDIIWRVKQRFGMPTAAYH
                     VSGEFSMIKAAAANGWIDEKAAALETTTAIARAGADLIITYFAKDLAGWM"
     gene            15883..16068
                     /locus_tag="P5316_01540"
     CDS             15883..16068
                     /locus_tag="P5316_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83667.1"
                     /translation="MTCKQLAIETIQKLPDSATWFDIEERIQFLAAIDKGLDDVKNGR
                     VIPHEDVKESLKQWLSE"
     gene            16053..16352
                     /locus_tag="P5316_01545"
     CDS             16053..16352
                     /locus_tag="P5316_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRR83668.1"
                     /translation="MAFRVIWTESASNDLRQIVQFIARDNRDAAAKLAERILQHVEAA
                     SALPWANRTVPEKGDSSIREAILRPYRIIYLVEERRENVHILRIWHAARGIPEIE"
     gene            complement(16405..16926)
                     /locus_tag="P5316_01550"
     CDS             complement(16405..16926)
                     /locus_tag="P5316_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83669.1"
                     /translation="MASRRPGIARCSESRVGRRVRKEVFTYDNGHSGERFKPVIPHTS
                     LADDWRSGAVDGDSSRRRGAPAEPLEPQWYGGGPFLRQLPSGGTLLSYQESADGTVDR
                     CRMAVCIGDENARNFTNKTYPIPLGPVRNQAWNSLFVKDARTATAVSTATINGVRGIW
                     AIDGRVARQAKSE"
     gene            17762..18493
                     /locus_tag="P5316_01555"
     CDS             17762..18493
                     /locus_tag="P5316_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR83670.1"
                     /translation="MRNTPMLLAGCLFGFLMAGPVWADDVLPPDWRGNYGTITAEWDY
                     WGQPGQGPGNSVLYPGQIVANPGGFDPMQPVAYAYWNSSVYVHDVLNGRQSVLEINPG
                     GGLGPVQFGLLNYNWPNPEKRIRIQITFQGSGVVDFYTGSGSDPPTTVPWSPTWPWTR
                     IDAVVSASYAHPDGWQTNAYDLVIEPNPAWEVIALDWVYVGPGGWTAFIDQVVIDTWC
                     VPEPASAGLFAVAGLSALCHRRRRA"
     gene            18746..20284
                     /gene="xylB"
                     /locus_tag="P5316_01560"
     CDS             18746..20284
                     /gene="xylB"
                     /locus_tag="P5316_01560"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRR83671.1"
                     /translation="MSLLCGIDIGTSATKVLLCRPNGKVLATASTEYPVYTPKPGWSE
                     QEPEDWWKATVQGIAAACKKAKVKASAITGIGLSGQMHGSVFVDKSGKSLRRALLWND
                     QRTGAECAEIEQRAGGRDQLISMVSNVALTGFTAPKILWVRKNDPKTYGKTHRILLPK
                     DYIRLKLTGEYASEVSDAAGTLLLDIGNRTWHLGLLERLEIDKDLMPPVYESPEVSGK
                     VSAAAAKLTGLTPGTPVVGGAGDQPAGAVGNGIVTSGIVSATMGTSGVVFAHSDNPVP
                     NADGNLQSFCHAVPGKWCVFGCMLSAGGSLQWLRNTLFDKEIEKLRRTKKDLGQLYPA
                     MIEEAAQAPAGCEGLIFLPYLTGERCPYPDPQARGAWIGLTVRHKRSHMIRAVLEGIT
                     FGMRDQIEIMRSRGVAITQVRASGGGAASRFWRQMQADMYKANVVTINTREGGALGVA
                     LLAAVGTGEYATVPEACKAAIRVTETLKPKQASARVYDDLYPTYQALYRSLRDHFVSL
                     GSAC"
     gene            20590..20907
                     /locus_tag="P5316_01565"
     CDS             20590..20907
                     /locus_tag="P5316_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83672.1"
                     /translation="MKRRAYRFLVWLTMAFTGATLFQTYPMYPVSGDSGTGYYGCTRF
                     AANSIGNSIDFCWLLDCQNGFFGGLIDPCNSAAGAFLVDCDTYTPPTTDTTDNTSGTN
                     TTN"
     gene            20984..21364
                     /locus_tag="P5316_01570"
     CDS             20984..21364
                     /locus_tag="P5316_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83673.1"
                     /translation="MKTKNRFWKSAVRLVMYLSLFQAACITGCLSKVARNINPCGTIL
                     NCNPTEWDWMFHSGDYPDYSADPTCVIPGQCGTTWPPTEPGQLGSGGSGTTTTTTGTT
                     TTGYLGGYTGGYGGGYGGGYGYGY"
     gene            complement(21517..25119)
                     /gene="smc"
                     /locus_tag="P5316_01575"
     CDS             complement(21517..25119)
                     /gene="smc"
                     /locus_tag="P5316_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRR83674.1"
                     /translation="MFLKRLTCQGFKSFADKTDFDFLPGVTGIVGPNGCGKSNVVDSI
                     KWVLGDQSARSLRGKQMMDVIFNGSGTRKSAGMAQVDLLFDNTDRKLARDTDEVTITR
                     RLYRSGESEYLINNEQCRLRDIRELFLDTGVGVGAYSIIEQGKVDILLQANPIDRRHI
                     FEEAAGISRYKARKKEAERKLERVEQNLLRLEDIVEEVERRLRSIKYQAGKARSFQEY
                     DRKLREKRAAFSMAEYHRLSEKRALLAQEEARLEDEVTRLRTAISSAETRSSALDGDL
                     MVLDGEVRQIEQQILTNASEITANTERIEQSRQRIEELIEVRSRAQQRHAMERQRAAR
                     LRQQLAAEQADLDQLEDRINAARAAVESLAVQDRQLAEQLTGMRARSDDDKAGVIDLM
                     RRSAQISNEIQALAQRRQHLEAEKARLQARRQQVETELAGLRDARGGIDARAAEVAEE
                     IRSRNEQLDELKQRAAQLDLDRAELNGRIAAAKEYRSGLLSRRQVLMDLEQQLEGVDA
                     GVRELLHRKERDATGKAFAYVRGMVADLIAVDVANATLIEIALGEYDQYLVVEDSEAF
                     FRDAELFADFPGRVKAICIDRLPPFVDGRDFTQQEGFVAYAMDVIRFQKDAERLVRHL
                     LGKTIIVRTLADAIRMAGQNPPGYRYVTLTGELLDPLKAHCEMGPAGGRTGLISRKSE
                     LREIDVQLEEVDERIDSESGRLEETSTEMVQIEQAIEDQRRLLNESLRAEVEIDSLRH
                     ANENAVQRLMQEQPLIHSEMAAIDRQIAEATEKSNHCSQALSRLESENVDREERIRRL
                     DAQIAVLSEERSRLSEQMTQARVAVAELMQRRSLMAGALHGTREACQASEEAVRSAAR
                     ESEDSAGRIAQAERMILSAESRLAELYSLKEQRDADLLARQRRRQEIRLEIEQLAART
                     KVERSSLNECEEKLHLLRMESQEARIRIEDLVARVRDELGVDLAESYANYQHREQEQD
                     WAAVEAEIEELKLKIQRLGNVNLDAIAEQEELEKRSDFLGQQRADLRTSQKQLTELIE
                     HLNQECRERFTQTFETVRGHFQELFRKLFGGGKADIILEQPPEGQPLDVLEAGIEIQA
                     RPPGKELQSISLLSGGEKTMTAIALLLSIFRSRPSPFAILDEVDAALDEANNERFNRI
                     VGEFLDKSQFIIITHSKRTMGIADVLYGITMQEAGVSKRVSVRFDSTEDHIAAA"
     gene            complement(25615..27921)
                     /locus_tag="P5316_01580"
     CDS             complement(25615..27921)
                     /locus_tag="P5316_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HRR83675.1"
                     /translation="MLENFRRAALFILAVFICPAAASGQQNQAIYIDALGSGWQDWSW
                     NTTRDFNNASPVHGDGGKSIAVTYTAAWAGLYLHNNTNVLLTDYSAIRFWIHGGTTGG
                     HLIQVLPFDSAGHAGTAVGIPATTAGTWTNVEIAISSFAFPAFSGFAWQDRRGAAQPV
                     YYLDDIELVAAGPPPPGPGPALSVDVAADRHAISPLIYGWNFAGAQMAAELSLPINRW
                     GGNATTRYNWQLDTSNRASDWYFENIPDSNPNPGQLPNGSNADRFVEQNLATGTDSLI
                     TVPLIGWTPKDRAYACGFSVAKYGPQQKTDPWRPDCGNGVRTDGTYITGNDPADTSIA
                     ITPAFVSDWIAHLAGRYGTADQGGVRFYDLDNEPMLWNSTHRDVHPGPTTYEEIRDRT
                     IQYASAIKAADPAAFTLGPVVWGWCAYFYSALDGCGPGTDYASHGNAHFVPWYLRQMR
                     AYEQQQGVRILDYLDMHYYPQGNNVALSSAGDAVTQALRLRSTRSLWDPTYADESWIA
                     NVQDGPYVRLIPRMKQWVAENYPGTKLAITEYNFGGLEHINGALAQADVLGIFGREGL
                     DLATLWDPPQSAQPGAFAYRIYRNYDGAGRKFGETSVRAVSEDQGRVSIYASQRSDNR
                     LTIILINKTNGILTSSLSISSYPSAACAQVYRYSAADPARIVREQDVAVAGGTVSIQM
                     PASSITLLVVPPALAQADFDGDTDVDRDDYKHFQQCSTGPGLGPPSSGCANADLDGDA
                     DVDQSDFGLFQRCLSGPYILADPGCTSR"
     gene            complement(28129..30333)
                     /locus_tag="P5316_01585"
     CDS             complement(28129..30333)
                     /locus_tag="P5316_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83676.1"
                     /translation="MRKQFTCCAGLVLVCVWASAAYAQTIGDWEGVMDGWAIAPTAPP
                     GTTVAFSTTGVTRNASSLKVTVPAGGWQRAVYISLNTQELRDAFFSNYEFAVDVTRIA
                     AEWAGTETWTNGVQLIINAGSNSGTAWGIYTDLGWRASWNFANGNNTTTASWDYRHVL
                     PTINRNNLEWLELIFVTNYSDTAYTQTGSYYLDDAHFFPGLPLLGDWEQRRDGWYFWE
                     PPQVTHDYDAVGATLHDYSFKLTGTQAGWRNGPVLDMAASAARIDAFLANDVFSVDLT
                     RLASEWTPDPAVQWPGCTIHFIVNAGFDDGTASIWGDCGGAGNWDSRNGDWTTTLSWN
                     YSAYKQTISDHRTAISWFQLVMCLNNWGYTAGFVYHLDNARLSPPPPQPQGFYTINNF
                     DTADEVVDPTTQAAIWFQQNPETGPPTTLEWDPTVDADNSPTSGSLKVTVAWDPNSPP
                     AEWQTLVARDVFPGGENIPSGADLSAYSYFRAKVKVDPSSPMDVWGAFGWSNWVFRSI
                     GWSWDQWAGSGANLDTDGPLVDANGWMSFNVFMSGDPAPVPPRDLFRALTYQLGGSGE
                     RDGTPWTLDGTTIFWIDSISVGRFQPCHDQYHCEICNNRVDDNDDGLVDCLDSDCPGT
                     TVWCPLETVCDDGFDGEDLDGLVDCADPDCAADPNCTHNDPFADMDNDGDVDHNDFGL
                     VQVCLSAAPVPRGCGFLDRDADQDVDGQDLLAFEACASGPGIPADKACDDER"
     gene            31098..32846
                     /locus_tag="P5316_01590"
     CDS             31098..32846
                     /locus_tag="P5316_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR83677.1"
                     /translation="MPEVAIYQKLCKGKELAGEGRHAEALAVFEEVIRESTNASYRWD
                     ADIQAALAMANLKQIPDALKRLDRIIAECPLPEDLPNAQMAKAEVFSLDGQGEVAIRI
                     LDELVRTHTDIWPRICEEALVAKAAAHQRFGQIGLTRVTLDRIILDYPGSEDTQRSWA
                     EKQVKSLTDKHQAAQAERVGKMTAENAVLLDKIGAGETTLTASRPYVVTDLLAVAEGA
                     TLRMEPGVRIHFGVRGGIVVKGRIEATGTSERPIIMEPLDGDPDRDWWFGIRYQPAEA
                     GSTCRFSHCRMAGAETALETMGGAAELSDCVFDRCGRVSVKVGKGGRLAMRACKIEQA
                     RRVGVEAESSSVLVMDECQITAATSHGLLLQGTGKETRIHRTSINACGRNGIFLRNRC
                     SPVISECRLQSNLVNGIEAIDEASPIVTATACKENGKHGMLVKDKSQLSVASCAFVGN
                     KHSGFVAEGRCSGRITSNRMDGNEEHGLVLRLDCDPEVTLNSFAGNRRLGVLLQNAQP
                     AVFKNNAFEKNGTAALRNEGPGGIQAGGNWWGTADEKGVKAVIQDQTVNGEWGAVDYA
                     PWLSSPPVSGIPKVAD"
     gene            complement(33062..33739)
                     /locus_tag="P5316_01595"
     CDS             complement(33062..33739)
                     /locus_tag="P5316_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR83678.1"
                     /translation="MREFISALWQNVATSFRDGKGPARWVVMGTGGAVVVLFLGWLIF
                     AKSAEDFLDVPDLSEAQIRRIATELLLSENPIIRERAAAKLTSAGSKSVPVLDQVVLE
                     LDDLRLSEAALCVLLSVDRNAAAKTIESLMANADVEVRRMAVRSAGTCDDPKMAAILS
                     KALRDADSGVRLAAANGMARQKSRTGVADLERMLNDQSPTVRRHAARALQNLTGRDYS
                     QRARAPQ"
     gene            complement(33822..34664)
                     /locus_tag="P5316_01600"
     CDS             complement(33822..34664)
                     /locus_tag="P5316_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR83679.1"
                     /translation="MRVLRECLELRRQFDVESRLQIRSIEEGTLMYVHCIPGRSKKAF
                     TLIEVLVVVAIMALLMSILLPSLRNARALSRAVVCSTQLKQIFNATVMYSQTNDDRLP
                     YFGWYEAAQSGRHWWITQLAKNLGNEYAIYKCAIDEDPARMAVVREKGVLYMSQGNEP
                     NRFSMDITYRSACDTLEHYKLRDGTTGYRARKITSYKRPYWSILMVEANSKRDESTSE
                     CFRFKDDLQYIADPVYIRNYPFLKGFQRHLGKSNLMFADGHVERLTPKQIAAAAIKQE
                     HYLD"
     gene            complement(34824..36269)
                     /locus_tag="P5316_01605"
     CDS             complement(34824..36269)
                     /locus_tag="P5316_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRR83680.1"
                     /translation="MIKRHRFTLACCLLLSSASCRQKAVPVEKPLLAEELPQFDQPLL
                     YAAWNQEGSWPQFCHDPLHSGRSDVDLGSAEMELAWRFRPTTRAWTYQPGFAVWSSPA
                     SGTIAGRPLVVLGSQDRNVYAVDARTGEKAWEFRPGAPVFAAPALGVVGGRAMVFAAS
                     LNRSIYGLDAVDGSRIWQCETASWSFTAARSVMASPTVVVDNRGEVLLLAGVWNADRS
                     ATQNVQSGECLAISAANGEVKWRRRLGSVPVSSPAFARVHGVETVFVACGDGTVHALA
                     LADGAVLWTAVLNEESHSSPSIGLVEGLAQVCIGSRLNSVFGLSASSGARRWRAETGY
                     WVDATPAWLKEPSDGGPRAAVVAGSYDRSVYKWDAHDGTLQWQVLTGNYAYSSCALAR
                     IGQTPVVLAMSWDEHLYLFEAGRGQMLWRYQGGPLLWSHAYMGDSLWASPAVIKAEGR
                     PMLLFPAFDGVLYAFRPIQPTAASRSADPRH"
     gene            complement(36266..37315)
                     /locus_tag="P5316_01610"
     CDS             complement(36266..37315)
                     /locus_tag="P5316_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR83681.1"
                     /translation="MALLGFIFKIFHEPLVPVAGTVDLAPFLVMVSLLSVGAVFMRLR
                     PAWRRQWLRRIVQSVGLLAFVFGLHPCACMVRDLLRGGAWINYNNVWAFQSMMLVVPV
                     LAFGMAWGRAFCGWVCPIGFVQELVGKLGEPLCREGSSRGVLWVRFVMACSLLLGTLA
                     AYAFIRPHNEPLLQGIPAAYLMVLSILITFSVADRQWEVGLRRVRYASLAFFVGGTIL
                     SIHMQAAFCVLFTNDRQYTMLLLLAGVLAAAVILSSAWCRFLCPEGALLSLVTRVSGW
                     RVRLEPSRCTKCNACRQVCPTDAIEAGHVDDSACLACCRCIDACPSGALDVEEAASDQ
                     RSSPEPVEPAKAARA"
     gene            complement(37318..38391)
                     /locus_tag="P5316_01615"
     CDS             complement(37318..38391)
                     /locus_tag="P5316_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR83682.1"
                     /translation="MLRVEGLRRKAGDFRLCVDRWEVPAGTYLVLVGPSGAGKTMLLE
                     TIAGLEQPSEGRIWIGDRLVTDLPSESRGIGFVYQDCCLFPHMSVRQNIDFARRYHRG
                     GDRQDGPATTELAEMLGIGGLLDRRPDSLSGGERQRVALARALSIRPKLMFLDEPLGM
                     LDPVSRETVATELRNCHRRFGTTTIHVTHDHAEARSLGDEVAVMLAGLLVQSGPAEEV
                     FRKPHTTALARFLGCENLFECQVVRDARAGQVELSLGGQVLRFSSAISGRAIACVRSE
                     EVRVQPWAGAMPQTASCAILPGTLIESSLRGPLIRMVVETDGMRWVSLAGRALSDAER
                     CRVGMKVAVLVDAKAVHLIPAET"
     gene            complement(38378..39736)
                     /locus_tag="P5316_01620"
     CDS             complement(38378..39736)
                     /locus_tag="P5316_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR83683.1"
                     /translation="MSDERSGPVVPSVISVGRPRSNWIPLPRQRIISMLVGAAILALV
                     HLLVGSAWARITGSADQPGGSLRPQINLALFVANLVVLGSVLLSHRSLNWPRVIAYAV
                     GYAVIVWLLWPLMRIMPENALLLVLSLVAYAGLLGHPWLVGYLYLFLLSQRFMPAYLY
                     SAFLLAALFYTTLPPAVRAWHHGRERFVPLCHLAGMMFLLLLLLPIIYFCTLTSAQDI
                     HARLKEADVLSALAVSLRTSAIAAAVVMLFGVPLAYAMVRRCFPGRALIDSLIDLPIV
                     LPPPIAGITLLFFLGPKTPLGRFLDQHFGLQFFDSEWGIIAAQVFVGSPFLIRASMVA
                     FEAVDVRYEKVARTLGASTASAFLRVTLPMSVRGILIGTILTWFRAMAEFGSLRVLAN
                     RPRTIPVLAYERFIEFRQSESQSVAVLVLLLSLGVIVGMWIIRAMPRLLGRAIGAANA
                     AR"
     gene            complement(39951..41072)
                     /locus_tag="P5316_01625"
     CDS             complement(39951..41072)
                     /locus_tag="P5316_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83684.1"
                     /translation="MRLLSIILASLAAIIAVTVWLQREATMSAGPSPFPPKLLHVVGP
                     GNTLASIARQANNPLVFSYDSQTRTAVSRATLIIEGELQLGKPGDPASGECLEMATQV
                     CGDLRIEVRAGGVLRLDHSTLRTVSQVLSNTACSRGYALFVDGSLLMDHARISYLSGS
                     TSQCLRQSAQAVIRDSSFAYCDGSALSCVNVDGGRIIIERSELLCSGNYALIVQGSGG
                     APLVLRDTVFDSQLGDVLLTGESAQVVLIDCVFDPAKIVFNRESGELTVQWTRRFKVI
                     EADGRQAVADAVLRAKPRNGSGDLTVVEARTDGAGVASLTLTDRVILPGAQGRNTSPQ
                     VRYDVTVVGPGGRGMARLEDLAVRGKEIKGSEPITLELR"
     gene            41782..>42216
                     /locus_tag="P5316_01630"
     CDS             41782..>42216
                     /locus_tag="P5316_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRR83685.1"
                     /translation="MEKAIAWCRQTGITHVYLETFRDGYTAERAALLAAKQRFRQQGF
                     LVSGCVTTTGIGRKSVNGWMFPCFTEQAGLDKLTQIFEYAAGLFDEIMIDDFLATRCE
                     CEDCVRARGDRSWDRFRCDLMVDVSRQRILEPARRVNPAVSLI"
BASE COUNT         8087 a        13033 c        12913 g         8183 t
ORIGIN      
        1 tggaggtcag tttgggctta gggggtgcca gaaacgcctc gatttgctcg tccgtgtagt
       61 gttgcttgag gtacgagatg acgtcgaaac tcccccgcca gcaatcaacg atcaagcggc
      121 agggccggcc ctccgggagc atccggcagt agccgaatcg gatctcatgc cccaggaggc
      181 ggcaacgacg ttccagatgg tcgggcgaat ggtcgttcgg ctcgggcatt tttcggcccc
      241 ttccatcgta actgatgcgc attatgcgcg catgtttccg gagcacagcg cgtttcagtt
      301 gcggccggca aaaaggcgct ccgtcccggc cactcccccc aaagcagggg accggggtct
      361 cgcaaccagc ccgatcagtg ccggtcccgt tacctcggtc cccggtcgca tgttcctgat
      421 ttccaacggc cgccgggccc gcttgcctac cacttcccgg acttcattac cttgggcttg
      481 ggaaggtcct tgatggccgc cagagacctc tgcatttcat cttcgggcag cgggaagtcc
      541 tcaagctggc cacggaagta tttgtcgtag ccgagcaggt ccatcaaccc gtggccgctc
      601 cagttcatga ggatcacctt ttccttgccc tcttccttgg ccttcctggc ctcgtcgatc
      661 gcgcaggcga tcgcatggct ggtctcagga gctgagatga atccttccgt acgagcccat
      721 agtacggcgg cttcatagca ctcgagttga tggtaggctc gtggtgtgat cagtccttcg
      781 ataatcgcct gactgaccag cggggcgata ccgtggtacc gcagtccgcc ggcatggatc
      841 gaggggggaa cgaaagtgtg ccccaacgtg tgcatgggca acaacggggt catgcgcgcc
      901 gtgtcgccat gatcataggc gtacggtccg cgcgtcatcg tcggacaact cgacggctcg
      961 accgggatga cctcgatgtt cgcaccggcg atcttgtcgc agacgaatgg aaacgcaatg
     1021 cccgcgaaat tgcttccacc gccggcacag gcgatcacgg tatcgacctt cttctctcca
     1081 atcttggcaa gctgcttctt ggcctccaga ccgatgatgg tctgatgcag catgacgtgg
     1141 ttgagcacgc tgcccagcga gtagcgggtc ttcccgctgg gatcggtgac cgcggcttcg
     1201 atcgcttcgc tgatggcgat gcccagactg cccgggcaat tcgggtcttg ggcgaggatg
     1261 tcgcgcccgg ccttggtttc gttgctgggg ctggccacac atttgccccc ccacgtttcc
     1321 atcatcagcc ggcggaacgg tttctgctca aagctgacct tgaccatgta taccttgcat
     1381 tcgagtccca gaaggctgca ggcaaaggcc aaagcggatc cccattgtcc ggcgcccgtc
     1441 tcggtggtca ggcgcttaat gccgaattgc ttgttgtacc aggcttgggg aaccgcggtg
     1501 ttgggcttgt gactgccggc aggactgaca ccctcgtttt tgtaatagat cctggcgggt
     1561 gttcccaagg cctgctccag acgccgcgcg cgatacagcg gactgggacg ccagagataa
     1621 aggatttcga ggatctcgtc cgggatgttg atccatcgct cttggctgac ctcctgctcg
     1681 atgaggttca tggggaagac cggagccagc atgtcggggg agatgggctt gccatcgggc
     1741 cccagtggcg gttgcagcgg gttcggcagg tctgccgcca gattgtacca ttgccgcggg
     1801 atttcgcttt cgtccaacaa cacctttgta ttcatctcgt tgtcaccttt cttgcagaca
     1861 gagtcttcct cgtcatgact gaacccaaga tccgttccgg gtagagtccg atttgaaaca
     1921 ctcaggagag atcacccacc aaaccgctgc cggtcagcgg ataagaccaa tccttgcccc
     1981 ggctccatcc tgcgccgcct gcgttcgtat gctagcgggc gggcttatca catgtcgaga
     2041 tggcattctc gtgccatatg cgaatgccgt caaggacgcc ccgtgggtgc tcgctggtcg
     2101 atggcccgac cggattcagt ctggctgcct gaaatcagtt ttgaaaagaa gcaggagcgg
     2161 ttgagccggc ttctgcccgg gttgagatca gaagcttgcc gtggccgggg gagcacgcgg
     2221 atgcctttcg ccgccctgag agctcggagc ttaccccgtt gcgcgtgcat agaagatgca
     2281 ggtctttggg gaacggcagg tcacaacaat cctgcccttt catccaggcc gaacatgatg
     2341 ttcatgttct ggatcgcctg accggcggcg cctttgatca ggttgtcgat agccgaaatg
     2401 acgatgatac gcgacttgac cactcgggcg ctgatgtcac agaagttggt gtgagtcacc
     2461 agcgatgttc gcggaaggtc cttacgcagc cgcacgaaag gttcgccggc gtaagcttca
     2521 gcaaagacct ggttgacatg ctccggtgaa acgggttgga gcggcttgag ataaatagtc
     2581 gagagaatgc ctcgttcgat cggcagcagg tgcggggtga agatcaccga ggacttgccg
     2641 ttccgaacgt acgcatcgag agtacgctcg atttccacca tgtggcgatg cgtgcccacg
     2701 ccgtaggcct caaagttctc gtttcgttcg gggaaatggt gctccggttt gggctcacgt
     2761 ccggccccgc tcatgccgct ggcggagtcg acgatgacct cgtgcgggtc gatcagaccc
     2821 gctttgagca acggtatgac gcctagtgcg gccgatgtcg ggtagcagcc ggggttggcg
     2881 ataaggcggg cgtcgcgaat tttctcgcgg tagaactcgg gcaggccata aactgcgtgg
     2941 gccaggtttt ccagatcggt gtgcttcttg ttgtaccact gttcatagtc ggccggttct
     3001 ttcagccggt agtcggcgga aaaatcgatc acgcgaagcc ctgcctgcag cagcgtgggc
     3061 acgaactggg tggacacagc gtggggcagg caaagcatga ccacatccgc ccgtcccgcc
     3121 agcgatgcgg gttcaatctc ctcgaccggc agctcgatcc ggccgacgaa ctccggaaag
     3181 acttcatgaa tgtgcggtcg ccacatccag tctttccgcc cgcacagggc ggcgatttca
     3241 acctgcggat ggcggagcaa ccagcgaatg gcctcgcgcg aggtgtaggc tgttgctccg
     3301 atgacggcta cgcgaatctt ctggtttggc atgggactgt tcccgtactg cacgtcttcg
     3361 attcctgcgc cgaagaaaca tggccttgga agccgaatga caaaaacgag ttgtcagtga
     3421 tcagtcatcg gtgttcagtc gcgtttgcga gcgccaaccg aatactgacg accgacaact
     3481 gacggcttct tgggggtctc tttctccatt ggtcatgagc acctgtggtc ggcatagaaa
     3541 acgaccccgg caaggcatgg ccggggccgt tgcgtttcca gacgccgcct caaccattta
     3601 acgcttcgag aactggaacc tgcggcgggc gccgcgctgc ccgtacttct tacgttcctt
     3661 catgcggctg tcgcgggtca ggtagccccc gccacgaagc accgcgtcaa actcgctgtt
     3721 ggcgaccttg atcgcccgcg cgatccccag gacgaccgcg ccggcttggc cggtgggacc
     3781 gccgccgctg gtgttgacga aaacgtcgac cttgccgagc aggtcggccg cccgcaacgg
     3841 cgccaggcaa tcctgtcggt cgcgttcctc cttgaagtac tgctccatcg ggcgcttgtt
     3901 gacctcgaac ttgccggtgc ccggacggat ccgaacccga gccacggcct tcttccgacg
     3961 gccggtgccc cagaagtact tcttcttcgg atccggctcg gggatggttg ccgaacctgc
     4021 cgttggtgtc gccttttctt tgacttcttc gctcatcgat gcagacatcc gttggtaaac
     4081 cgctcggtcc gcctcgcgag gcgaccgatc atcaatcctc acgaaatcac accctcgtaa
     4141 gttccagcgg cctgggttgc tgggccgcat gcgggtgctc gctgccgcgg taaatcttca
     4201 gtcgcttgag catcccggcg gcgagcttgt ttttgggcag catgcgtcgg accgccagcc
     4261 gaagaacccg atcggggtga gccttcatca acttggcgat cgggacgatc ttgtgtccgc
     4321 cgggataata ggtgtagtag tcgtaggttt cctgctccaa cttgcggccg gtcagcttga
     4381 ctctctcggc attgatgacc acaacgaagt caccgctcag gacatggggg gtgtaggtcg
     4441 gcttgtgctt gcccatcaag atggttgcca attcagcggc caagcgtccc aagaccttgc
     4501 cgtcggcgtc aaccaggtgc cactggccgc cggcgtctcc cggcttggcg acataactct
     4561 tcattgctgc ggtatacata ggtgcaaacc actccctcac ccgatcttgg ggactctctg
     4621 ctgaccattt cccctgccta caaggcagag ccccatagtt tagccgatga tggcctcagg
     4681 tcaaggcggc ccattctgcc gccgagcggt cgaggcggga ccccgccggc cgtcagtctg
     4741 ccggcgtttt tcacggcccc caaaagcggg caagtcgccc gtgcctccca cggtagatcc
     4801 ccgccgcacg tgtcaaggac cccgaagtca gggggaaagg ggttgggcaa ccttgagctc
     4861 acctccccgg tccccggttg tcccgcaatt cccaatatct cgcccggcaa acttgttcag
     4921 gtccgggcat ctgtacccct tgcgcgcata tttccggaac gtagtgcccg ggggttgcgg
     4981 cccgcaacaa ggccgtccgc cgcaggctcc tggaccgcac ctaaagctcg tagaggcaag
     5041 gccttacgct tccaggcccg ggagcccatc aggtcacctt ggaaccgggt tcgatgtcag
     5101 catcgggctg gagcaggata atacgtgtgt ggtccggggt gctggcggcc agcagcatcc
     5161 ctttgctttc cactcccctc atcacgcggg gagccagatt ggccaccacg acgatgttcc
     5221 ggccgaccag gctggccggg tcgtaatgcc cccggatgcc ggcgcagatc tgccgttgtt
     5281 cgtatcccag gtcgatcttg agaaccacca gtttatcggc gttggggtgg ttggaggctt
     5341 cgagcacctt ggccacccgc aggtcgatct tggcaaagtc ctcaaactga atcacgcttg
     5401 cttgatcact catggtcctg actcccgaaa gaccgccgtg gcaatgcttc ggcgggttgt
     5461 cttcatgggg caacgggagt gtactgcggc aactctccgg cgtcgagggc agccccaagg
     5521 cgcttgtgtc cattccggaa ggcgatatcc tgcctgttcg catttcgcgt tcaggaggtt
     5581 ttcatgttcc gtatctggtt ggcgtcggtt gcggttctga gcggttggtt ggctggagct
     5641 tcaaatgccc ccgcaagcgg cccggccacg accccggggt ctccggccgc tgctcaggtg
     5701 cgatactgcg acgtcacccg cttcggggcg gtgggcgacg ggaagacaaa gaacactcag
     5761 ccaatacaag aaacgatcaa tgcggcaaac gaggcgggtg gggggattgt cctcattccg
     5821 ccggggatct acctgacggg caccatccac cttcgcaacc atgtcacgct gcacctgttg
     5881 cctggggcga cattgttggg tagcaccgat ctggcggatt atccttccac ccaagtgacc
     5941 tttcgctcgt acaccgacca ttacgtttgc cagagcctca tcgccggcga aaatctgcac
     6001 gatatcgcga tcacgggcaa agggatcatc aaggggcagg gagcggaccg ggctttctcg
     6061 gcctcggcac ctgattgggg tttccgcaag cggccctaca tcatccggtt ggtctcttgc
     6121 cgaaacgttc tgatcgagga tgtaaccttg cgcgattcac ccatgtgggt gcagcactac
     6181 ctggcatgcg acaacctgac cattcgcggc gtgaccgtcc ggagccgcgc caacaacaac
     6241 aacgacgggc tggacatcga ttgttgccga aacgtccggg tgacggggtg cgacatctcc
     6301 tccggcgatg atgcgctttg cctcaagagc actgccgacc ggtcctgcga gaacatcgtt
     6361 atcagcgact gtgtgttgag cagtgcctgc aacggcttca agctcggcac cgagaccaac
     6421 ggcggattca agaacgtcac tctcaccaat tgcgccatgt atgacgtcaa tctggccggg
     6481 atcgcgctgc tgatcgtgga cggcggcacg ctggatggcg taacggtcag caacgtgacc
     6541 atgcgaaacg cggggtcgcc catctttctg cgtttgggca accgcgcccg gccgttcaag
     6601 gacaacatgc cctcgccggg tgtgggccga ttccgcaacg tggcaatcag taacatcgtc
     6661 gccacggggt gcgagcgaac gggctcgtcg attgtcggtc ttccggacca tctcatcgag
     6721 aacgtgacgc tctcaaacgt caacctgacg tacgcgggag gggtgacagt cgcccagatc
     6781 cccgccgaga tccctgagca accggccggc tatcctgaac acaacatgtt cggcgccctg
     6841 ccggcctacg gtttctactg ccggcacgtc gacggcctga cgcttcgcga tgtcgcggtt
     6901 cgatttgcca agccggacca acggccggct ctggtgttcg atgatgtccg gaatctggcg
     6961 gtcagtggcc tgcgtgctca aggcaccggc ggaacgcggg caacaatcgc gatgaaagct
     7021 gtcagccgcg cgctgatcag cgaatgtgtg gctcctgcgg acgcgccggt cttcacctgg
     7081 atcgacgaag ggtgtgaacg gatcagcgtg atcggcaacg acttttccga gactgcccgg
     7141 ccgttcgtct ttgcggggtc ttccctggtg ggctgcctgt tcgagtcggc taaccggacc
     7201 ggacaaacac ctgttgcccc ggccgcctcg aggaccgctc cggctgaaga gtagggacac
     7261 ggatcacgcc aaggcccaca gaacaagatg cgctgccggc agacggactg gcggtcatca
     7321 gggggatttg ccggagtcac atggcgaaac ggtccaaggc gggttcagac cggcaagatg
     7381 cgggtcccac acccgattcg cggtgcacgc ctattgcgaa tccaatgcga tttccgccca
     7441 ataggcacct tcgtgttgcc agtcgtcggg ttggttgacc aattcaagct cagcgctctt
     7501 gcccccaaac ttgctgaggt ctactttgat cgtcatccag ccttcggtgg tcgattcctt
     7561 gccgaccagt ctctcgatga gcgtttcgcc gttggccttg acgaccagca gccaatcgcc
     7621 ttcttcgtgg tggccgacca cgagccgcaa ggtggtcttc ttgtttgcag ggatgtccac
     7681 cgaccggtac agcgtgcagc cggttttctt gttcagcgga tgactcacat acacgttttt
     7741 cctgccgcgg acttcgtcat gcagccccgg gttcatttcc cggccgcagt cgcggatctt
     7801 ccagcccggg gcgaacttct cgaatgcccg tttgggacca agcgcggtaa tcctggccat
     7861 ttcgtccgcg ctgaacatac tgccggcgat ggggccgggt tcccagctca attcaagcgg
     7921 gctgggcttg ggtgcctgta cggggatgac gaaaaactcc tcgccggatg cgtccttctc
     7981 gacccgcccg cccgcctgca cgagcgcctg gcgggccagc ttttcgcaga cttcgagcag
     8041 ggcggggaaa ttgtaggctg tgtggctgaa aaccttggtc tgatcaagct ctttggtgaa
     8101 ccggtcgggc agcttggaga agccgatcgt ggtgaacaac acccccgcgg agctggatgg
     8161 attacagtcc gaatcctggc cggatcgaca ggagatgatg atcgtcttgt cgaggtcgcg
     8221 gttgccgaac agcaggccca tcagcacgta ggccccgttg atcttgacat cgatgccgcc
     8281 gttggaggcc ttctggtact ccgggtcctc gcggtacttc ttctgagcct tgtgccatgc
     8341 cttctcccaa tcggtcgggt cttcgcggta ccatttcaac atgtcgcgga ccatttcggc
     8401 gtactggctc ttttcaggga tgcacttgag gccggcctcg acgatcttga ccgggtcgtc
     8461 ctcgaaaaac gcctcggcat acatgcagcc catgaactgg ccgccataga cgccgtcgcc
     8521 gtagttcatc aggcgtccga acttctcgcc aagcttgatg gcgacgttgg gcagaccggg
     8581 ggcgatcagg cccgagtagt cggcctcgat ctggtagtcg atgtcgttgg ggcacttgtt
     8641 gaacttcggg tggctcgaat ccggcggtgc gatgccgtcg cgaaggttgt ttcgccccgc
     8701 gttgttcgcg caccacagcg ggtacttgct gttggcaaag tcgatcccgg cctggcgaat
     8761 cgaaacatcg aggccgtatt gctccattga tcgaaggaag gtcatctcga cgtacaggtc
     8821 gtcctggccg aaggcgttgt tgatcatttc cggcttccac acgggcatct ggtcctcggg
     8881 gatgatcctg tctgcaaacc tgaactcggt gggtgcgccc caagaaacgc cggcgatctg
     8941 gccgatccaa cccgctttca tcttgtcccg gtaatccctc actgacaggc ggtggaagtc
     9001 ggcgccatag gcggcaggca ccgtcaggag aaaaacagcg agcgtcccgg cgaggaaccg
     9061 ggatcgacct ggggttggat gaacgcgcat ccggatcatg atcagatctc ctttgccttt
     9121 cgcgacgcaa aacatggttc tcagccgccc tgaggatagg cgaccatcgc tgcgttttcc
     9181 agcggcggat gaggtgaacg accgtcggac cgcctgccgg gtcgggttgc aggcttgaca
     9241 cctggggccg attccagtct cttaccatcc cggaaatgcg acgtcatgcg cggtccgggc
     9301 gagcaaatgg aactgtctca tcacaatctg gtcaactttg ctcatctgga ccatctctgc
     9361 gaggaggttg tctgcaacgg tgaggcctat cggatcgtcc acatctattg tccggccccg
     9421 gactacaacc gaacggccga tcccgaggaa gggacggcct gcgtggacga cgcggctcgg
     9481 gcggccgtgg tgtacctgcg gcatttcgag atcaccggcg acgagcagtc gcggatcaag
     9541 gccgaggggt tgctccggtt catcctgtac atgcagacgc cggaaggcct cttctataac
     9601 ttcgtgcaca ccaaccggct ggacatcaac acgactcatg agcgcagccg ggccgacggc
     9661 gtaacttggt ggatggtgcg ggctatctgg gcgttgggca cgggcgtgcg ggctctatgg
     9721 aaggccaatc caacgattgc agcccagtgc gaggaaagct tgcgtcgctg cctgccggcc
     9781 ttgtcacggt tgttggagcg ttacccggaa tgcgagaacc atcgcgggcg ggttattccg
     9841 acgtggttgg tcaacaatga tggcgcggac gccaccagcg agttgctgct cggcttgacc
     9901 gcgtatcacc aggcccggcc gtcgtccgag attcagacga tgatcagccg cttcgcccag
     9961 ggcatctcga tgatgcggta cgggtccatg aatcagtcac cctacggcat gcacgcctct
    10021 tggcgcgacg gctggcatct ttggggcaac tcgcagacgc aggccttggc cgaggccggg
    10081 attctcacca gcgccaaaat ggaggctgag cacttctacc cgcgccttct ggtcgagggc
    10141 tacctgcact catttacctt cgacgatctg cacaatatcc gttatttcga gcgaatcgcc
    10201 tacggcgtgc gggctgtggc ggtcgggctg gtccgcttgt tcgaggccac gggcgacgag
    10261 cgctatgcaa tcatggccgg gctggcgggc tcatggtttc tgggcaacaa cctggcgggg
    10321 aaaccgatgt acgatcgggc caccggccgc ggatacgacg gcttgcacca ggaaggacgc
    10381 atcaattaca acgcaggggc cgaatcaacg accgaggcct tgtatacgct gctggaagtc
    10441 gggaatcatc ccgaggccgc ctgttggctg aacgttcgcc ggcaggaaaa gcagcgggaa
    10501 acccgcgaag gagtggatta tttctatcgc gtattcgagg acgccacaaa ccatcctcca
    10561 cgtcgtatcg gtctcatcct gaacctgacg cacgaaacct ttgaactgat gcagggtgat
    10621 gcacttgatc gattcctcgg gtctgcatag aagctatgcc ggaacctgtg ggacggggtc
    10681 gcgacagatc ggttttcggc gattggggat aggcttgcag gagcagaccg gtagatcgga
    10741 ttccgcccgt cacatcgtcg aatgaaaatg agactcggct tccaggcggt tcacgccgga
    10801 attcagcatc tgatgcgcct tctacgccga gccgtgcggg ctgtcaccgg gcggaggttt
    10861 gggtctccgg ctgcggaaca gaaggcgtat gggtacttcc tcgaagagca actgttcgcg
    10921 gaggcggttg agaaggaacc tttcaaaacc tgggtttacg cgccctgggt cattgacgaa
    10981 aaaggcgatg gttggcgggc agacggccac ctgggtcgcg tagaagacct tgaccggtcc
    11041 gacccctcgc ttcggcttgg gcgggttggc gttcatagcc tcgttgacga cttggttgag
    11101 ctgaccggtg gggatccggg tgcgggcctg cttgaacagt gaagcggcca cgtcgatcac
    11161 cgcctggacg ttgcggttct gtgtcgcggt ggtgaagacg atgggggcga aatccagtcc
    11221 cggcagcgtc ttgtagaggt attcttcgta atcccccgtg acggctctgc ctttggccag
    11281 atcccacttg ttcacaacca gtatgcaggg cttgaactgc tcgacgatgt acccgccaag
    11341 agtcttgtcc acgtggctga cgtcctcggt tgcgtcaatc atcaacaaaa cgacgtcggc
    11401 ccgacgaatc gaaagctgcg cgcggtggta gccgtagtac tcgatgtcgt cggctatctt
    11461 gcttcgtttg cggactccgg cggtatcgat ggccacatag acatgcccgt cgcgttcgaa
    11521 acgtacgtcg acggcatcac gcgtcgtgcc gggcacttgc gaaacgatga cgcgttcctg
    11581 gccggccagg gcattgatga acgaactctt gcccacgtta cgacggccga caatggcgat
    11641 tttcatcgtc gggtcgggcg gggccgcgcc gtcgctgaac ggccggacga tctcggcgat
    11701 ccgtgtcttg agttcgctca gtccgagatg atggaccgcg gagacgggca tcggctcgcc
    11761 gaagccgaga cgaaccaggt cggccagctc ggtgcgggcg gcgggggagt cgaccttgtt
    11821 tgccagcagg atgacgggct tggagcagcg gcggagccag tcggcgaccg actgatcgag
    11881 cggattgact ccttcttggg cgtcgaccac gaagagaatc aacgttgcct ggtcgacggc
    11941 atagcggatt tgctgctcga cctgctcgcc aaggtcgtca cggtccacga cgccataacc
    12001 gccggtgtct acaaggtcga agtagatacc ttcgagttcg cacaaagctt ggacgcgatc
    12061 gcgggtgact ccggccgtgg gatcgacaat gctgatccgg cggccggcga gcacgttgag
    12121 cagcgaactc ttgcccacgt tcgggcgtcc gacaatggca accatcggca agggcatggg
    12181 ttgtcggcct ccgcgaaccg tcagaagtca gttcctgcaa acgatcatgg ggacggcggc
    12241 ggcatcaacg gactggggcg gtcgcggccg gagcactggc cgggccgctg gtgggagcag
    12301 gccgctggat gcccaggccc cgctggaagt tgtagggggc accgttcagg atgccggcca
    12361 cctcggccgt cccgatccag gcgtccatcg ccttttccat cttggcatta cgctcggttc
    12421 cgccttccgt catggcgctg gttacatcgg gttgcgtctg accgccggac ggtctcacca
    12481 ttgccttgag ttgggcctcg gtgatcagca ccacgcgctc caactgctcg cgtttttcac
    12541 gccctgcttg ggtcttggtg tagtggtata ccgcgtcggc caagagacgc gcggttcccc
    12601 ggacgatatc attcttcatt tgttcattgt tgatctgggc gaacttgcct gccagccaga
    12661 ttgtcacatt ggcgtcggcg acggcaggga attcgccctt ctgctcgaag cgggtcagcc
    12721 gcgcgttcaa aatggccgat agcaccggca ccacgcggtc aactgccggc ccgccgttca
    12781 tggtcaagac gcgatagagg cgatccatcg tggcggggtc cgtctcgata gacgcctgct
    12841 tttgcagggc cgggagtaat tcttcccagt cctgcggcga cagcttgctc ttgagcaatc
    12901 cctccgcagc caccaatcgc atgccggaga atgcgtcgtt gattgccagt ttgtagcaag
    12961 ctgtggcggc agggctgtca tgccgcctga gaaaggccaa cagtaccaca ttgccaagag
    13021 ccacgggatc tttgccgtcg gtcgccggct tgtacagcgc agcgaacgcc gttgccatgc
    13081 tctcgaccat gccggccttg aaagcactgg tcgcgtcagc ggcctgaata tgccccgtga
    13141 tctcgcttat gaaagccggg gcatctttca agggttcctt ggaggccatg atcagtctga
    13201 tgcgttgccg gcaccagtcc tctactcgtt gcctgtcgat ggtgccgaag ttgttcttgc
    13261 gtatgtcggc cagattgagc tgctgttcct gtccggtggc cgccgcggcg caggcgaaaa
    13321 ccaggcagcc ggtcaagact ccgcggccaa agcctcgtat acccatggtg ctgttcatca
    13381 gtctcacggc tcccagatcg tcgaaccacc catggctgac ccggattctt caaccgcatc
    13441 caatgggtga tcgcccaggt acccccacgc gggggcggcg gcgactgagg cgggtaactc
    13501 gtcgcaagcg aagctccacc gcagatacgg cccggaagca ccgggaccaa tgccgcgatg
    13561 cagtcacggt atacgccgcc ccggccgaag tcaaggaatc gggccccgcc ggtccggcca
    13621 aaggtcgctg cgcaaagagc ttatgcgggt tctgatgggt cctccgcttg acgctggccg
    13681 cctcgcttct gtacagtccc cgtatcatgg caagcgacgc gtcagccgcc ggttcccgtc
    13741 atgatcccca ggatcacccc gcgtgggatg gcattccagc tcccgcagtg cgtcgtctct
    13801 gcttgtacct gcgcgaactg gaggggttgc tggctcgggg tcggctgacg gtgtccagca
    13861 aggatctggg ccggacgttg ggatacaccg acgcccaagt gcgcaaagac ttggcgtatt
    13921 tcggccactt cggccagccg ggcatcgggt acggagtcga ggagctgatc gcccggatca
    13981 ggggaattct cggtacagat aggatctgga acgttgtcgt ggtgggagcc ggaaacatcg
    14041 gacaggcttt gtccacctat cggggattcc tgaaaagagg gttccggatc gtcggggtgt
    14101 tcgacaacaa ccctgccaaa gtcgggcagc gaatcggcga tccgcccggt gtggagattc
    14161 tcccgttgga tcgcctgccg gaagtcgtcg cgtcgcagga ggttcggctg ggcatcgtgg
    14221 ctgtgcccgc cgaagcggcg caggaagtcg ccgaggcgct cttggccgcc ggcgtgaagg
    14281 gaatactgaa ctttgccccg gcctcgctgg cggtacctga gccggttccc gtggttccgg
    14341 tcgaactcag ccttcagttg gagcaactgg catttcagat tcgagcccgg agcactcgcg
    14401 cccgggcccg atagaccgtc ggagattccc ggagcggtgc cttgccaaca ggcctgaata
    14461 ggagcttcga gggcatgagc cggcaggatg acagcatcga cgacccgcag gacattgacc
    14521 ttgaggaagg cggcgcaccg gatttcgagg attgcccgaa ctgcgggaag tcgatacctg
    14581 agtcggtcgg ccgttgtccg tattgtgggc aatggatcgc ctgtcccagt tgcggcgaat
    14641 ggcttacggg ggcctcgacc gcagccggcc gcgctcgcgg ttggttctgg ccggtggtgg
    14701 tggcgatcct cgttgcgatc atacttgtga tgtggaccgg cttgtgatgc gggcatctgt
    14761 gctgacggaa gaagacacat ggcttttcca atacaccgac ttcggcggtt gcggatgaac
    14821 ccgggattgc ggcggatggt gtgcgaaacc cgcctgtccc ttgaccgctt gatctatccg
    14881 ttgtttgtgc gtccgggcga gagcattcgc aaccagatca cgtcgatgcc ggggcatttt
    14941 cagttctcgg tggatacggc ggtcgaggaa tgtcgccgga tcgccgatct gggcattccg
    15001 gcggttctgc tgtttggcat cccttcgcac aaggacgccg ttggcagcga ggcttggcac
    15061 gacaacggcg tcgttcagcg ggcgattcgg cagatcaaga aggcctgtcc cgatctggtg
    15121 atcatcacgg acgtctgcct gtgcgagtac accgatcatg ggcattgcgg ggcaatcgtc
    15181 gaacgcgcgg ggcgcaagga cgtggacaac gacgcgacac tgccgctgct ggtcaaggag
    15241 gccctgtctc acgcggcggc gggcgccgat gtggtcgctc cctccgacat gatggacggg
    15301 cgagtcggag ccatccgccg ggccctcgat gaggccggct ttgcccacct gccgatcatg
    15361 gcctatgccg ccaagtatgc cagcagctac tacggcccgt tccgcgacgc cgccgagtcg
    15421 ccgccccgct tcggcgaccg caagagctac cagatgaacc cggccaacgg tgatgaggct
    15481 ttgcgcgagg tggcggctga catcgaggaa ggagccgaca tcgtcatggt caagccggcc
    15541 gtgaattacc tcgatatcat ctggcgggtc aagcagcggt tcggcatgcc caccgccgcc
    15601 taccatgtca gcggcgaatt cagcatgatc aaggccgccg ccgccaacgg atggatcgac
    15661 gaaaaagccg ccgccctcga aaccaccacc gccatcgccc gcgccggggc cgatctgatc
    15721 atcacctact tcgccaagga cctggcgggg tggatgtgaa agccgggcga ggagcggttt
    15781 gtttattccg cgcgcgcagc aggtggtttc agcgtccgcc cttatcagca cgagctttct
    15841 cgatcgcttg aagtcgccac ggctgcctgc taaactagtg tcatgacctg caagcagttg
    15901 gccattgaga cgatccagaa actgccggat tccgccactt ggtttgacat cgaagaacgg
    15961 atccagttcc tggcagccat cgacaaaggg ctggatgatg tcaagaacgg ccgggtcata
    16021 ccccacgagg acgtcaaaga aagtctgaaa caatggcttt cagagtgatt tggacagagt
    16081 cggcgtccaa cgacctcagg cagatcgtcc agttcatcgc tcgtgacaac cgagatgcag
    16141 ccgccaagct ggccgagcga atccttcagc atgtggaggc ggcatccgcc cttccgtggg
    16201 ccaaccggac ggtgcccgaa aagggtgatt catcgatccg cgaggcaatc ctcagaccgt
    16261 atcgaatcat ctatctcgtc gaggaacgtc gagagaacgt ccacatctta agaatctggc
    16321 atgccgcccg gggcataccg gagatcgaat gagggcggaa tctctgcagc gacacacgga
    16381 caggagcggc tacctgaagc agattcactc gcttttcgct tgccttgcca ctcggccatc
    16441 gatggcccag atgccgcgga cgccgttgat ggtggcggtt gagacggcgg tcgctgtgcg
    16501 ggcatccttc acgaacagcg agttccatgc ctgattccgc acggggccga gggggatggg
    16561 gtaggtcttg ttggtgaagt tcctcgcgtt ctcgtcgccg atacacacgg ccatccggca
    16621 gcggtccacg gttccgtcgg cgctctcctg atatgacagc agcgtcccgc ccgaaggcaa
    16681 ttgccgcagg aatgggccgc cgccgtacca ctgcggttca agaggctcgg ccggcgcgcc
    16741 ccggcgacgt gagctgtcgc cgtcgaccgc gcccgaacgc cagtcgtccg ccagggaagt
    16801 gtgcgggatc accggcttga atcgttcgcc ggagtggccg ttgtcgtaag tgaacacctc
    16861 tttgcgaacc cgccggccca cccttgactc gctgcaccgg gcgatgcccg gccgtcgcga
    16921 tgccacgggt ctcgcttcgc gagacgcatc gtgtcaaggg tcggaacaat catacgagaa
    16981 gcaaacgcgt gtgtcaccac tctcgtatct tacagcgctg attggtgcaa aggttttcgg
    17041 ccccgccggt acggcgcatc acacttcggc ctgggttaac gaccgcgtcg aatcctcagc
    17101 cccggtcggt catctcgaac ttccgccggg tatgtgtata gtaggcggga gactatagtt
    17161 tagaggctgg agaaaggtcg atactccttc cttgagcctg cactctacag ccttaagccc
    17221 aaggcggagc gcctttttgc cggccgcaac ccgagcgctc tgcgatccga aaacatgcgc
    17281 gcaggccgca cgtctctctg gccgtaaggt tccagaccgt cccggaccct ttgcacacag
    17341 cctaccgaaa caggcactca aggccggtgc gggttcccgg cctgccggac ccggcgtggt
    17401 agggccgccg ttggccaggt cggccggccg gccggtttgg aaaggacgcg gtgaacgtca
    17461 caagatcggg cttggcgcgg ttgacgaaat gagatacaat gcttgctgat caacaaatgc
    17521 aataagttct tgtatttcaa ctcgaatagt caccagggcg gggaagggag gatatcacga
    17581 ttccccgacc acgggcgatt gattggatct gccgcagtcg accctgcccg acgttgcggg
    17641 gtcgcagtct gccggaacct gccgaacatc atgtgttgcg tgttctcgta cgactctttt
    17701 gccgccaagt ggaggcaacg cgggagccac gcagaggcct ttcatagaag ggagaatgga
    17761 gatgcgaaac acaccgatgc ttcttgccgg atgtctcttc ggattcctca tggcaggacc
    17821 cgtctgggcg gatgacgtgc tgccgcccga ctggcgcggc aactacggca ccatcacggc
    17881 ggaatgggac tactggggtc agccgggcca ggggcccggt aacagcgttt tgtatcccgg
    17941 gcagatcgtg gcgaatccag gtgggtttga cccaatgcag ccagtggcat acgcatactg
    18001 gaacagcagc gtctatgtgc atgatgtatt gaacgggcgt cagagcgttc tcgagatcaa
    18061 tcctggcgga gggctgggtc cggtccagtt cggcctgctg aactacaatt ggcctaaccc
    18121 ggagaaaagg atcagaatcc aaatcacctt ccagggaagc ggcgtcgtcg atttctacac
    18181 cggcagcggc tcggatccgc ctacgaccgt gccgtggtcg cccacctggc cgtggaccag
    18241 gatcgatgcg gtggtgtcag cgagctatgc gcatccggac ggttggcaga cgaatgccta
    18301 cgatctggtg atcgagccta acccggcgtg ggaagtcatc gcgctcgact gggtttacgt
    18361 gggtccaggt ggctggacgg cattcattga tcaggttgtc atcgatacgt ggtgcgttcc
    18421 agaacccgca agcgcaggct tgtttgccgt cgcgggtctg tctgctctgt gtcacaggcg
    18481 acggcgagcc tgatcgatcg ggtttttgtg cctgttgaac tgttgcatcg accgggatcg
    18541 gtcgccggac cggtcccggg attcattatc atagcttttc gctcatccgc gcgacggcag
    18601 gcatgatcac gcagccccga cgccgttggg catgccgcag agcctttcgc ggaccgatcc
    18661 tcgccgcttt gacctcgtct ggtgggccag tatactgcgg ccgtttcaag cagcgcgaat
    18721 cagaggtttt gcgaggacac agaccatgtc acttctgtgc ggaatcgaca tcggaacatc
    18781 ggcaaccaaa gtcttgttgt gcagacccaa tggaaaagtg ctggccacgg ccagcacgga
    18841 gtaccccgtt tacacgccca agccgggttg gtcggagcag gaaccggagg actggtggaa
    18901 ggcgaccgtt caagggatcg ccgccgcttg caagaaggcc aaagtcaagg cctcggcgat
    18961 cacggggatc ggcttgtccg gccagatgca cggcagcgtc ttcgtggaca agtccggcaa
    19021 gtcgcttcgg agagcattgc tctggaatga tcagcgaacg ggtgcggaat gcgccgaaat
    19081 cgagcaaaga gccggtggac gagaccaact catctccatg gtctcgaatg ttgccctgac
    19141 ggggtttacc gccccgaaga tcctctgggt gcgcaagaac gatcccaaga catacggcaa
    19201 gactcaccga atactgttac cgaaggatta cattcggctc aagctgaccg gcgagtatgc
    19261 ctcggaggtg agcgatgcgg ccggaacgct gctgttggac attggcaatc ggacctggca
    19321 cttgggcctg ctcgaaaggc tggaaatcga caaggatctc atgccgccgg tgtatgagtc
    19381 tccggaggta agcggcaagg tttcagccgc ggcagccaag ctgacgggct tgacgccggg
    19441 tacccccgtt gttggtggtg cgggcgacca gcctgctggg gcggtcggca acggcatcgt
    19501 cacttctggg atcgtctcgg ccaccatggg aaccagtggc gtcgtttttg cccacagcga
    19561 caatccggta cccaacgcag atggcaacct tcagtcgttc tgccatgcgg tacccgggaa
    19621 atggtgtgtc ttcgggtgca tgctctcggc cggcggcagc ctgcaatggt tgcgtaacac
    19681 cttgtttgac aaggagatcg agaagcttcg ccggaccaag aaagatcttg gtcagctcta
    19741 cccggcgatg atcgaggagg ccgcgcaggc tccggccggc tgtgaggggc tgatctttct
    19801 gccttacctg accggcgagc gttgcccgta tcccgatccg caggcccgcg gggcgtggat
    19861 aggcctcacc gtccgacaca agcgatctca catgatcaga gccgtgcttg agggcatcac
    19921 cttcgggatg cgcgatcaga ttgagatcat gcgctctcgc ggcgtcgcga tcacccaagt
    19981 ccgtgccagc ggcgggggag cggccagccg attctggcga cagatgcaag cggacatgta
    20041 caaggccaat gtcgtcacga tcaacacgcg cgagggtggt gccctgggtg ttgcgcttct
    20101 ggcagcggtc ggcactggcg agtacgccac cgtacctgaa gcctgcaaag cggcgatccg
    20161 agtgaccgag accctcaagc ctaaacaggc cagcgccagg gtttatgacg acctgtaccc
    20221 gacctaccaa gccttatacc gcagtctgcg agatcatttc gtcagcctgg gatcagcctg
    20281 ctgaatgtaa ggccttgtca ataaatgacc tgtgacgagg caaggagacc ggtcgcccgt
    20341 ggttcggcca tctccggtgg gttggccgaa gaatagatat ggcggctgaa acgcggtcca
    20401 ctcaccaagc gtgagttgga agatcgtttt cgcgaaaggg gcagaaacaa ggcaccgggc
    20461 ccgggttgca gtgacactcg ggccgtccta tactgacagc gttgccgagt gggcaacccc
    20521 gcgcgtgact gacctgcgag aggtgtcttt ccgacagaaa ggtaaaccgc acgacggagg
    20581 ttattgcgta tgaaacggcg tgcatatcgt tttctggtct ggctgacgat ggctttcacc
    20641 ggcgcaactc tcttccagac ttatcccatg tatcccgtca gcggtgatag cgggacgggg
    20701 tactacggct gcacgaggtt cgcagccaac tccatcggca actcaatcga tttctgttgg
    20761 ttgctggatt gccagaatgg tttcttcggc gggttgatcg atccgtgcaa ctcggccgcc
    20821 ggtgcgttcc tggtagattg cgacacgtat acgccgccga ccacggacac cacggacaat
    20881 acgagcggca cgaacaccac aaactgaggc gggttgccgc cgaaggcgga cagaatcatc
    20941 ggttttgggg aacaacaaga ctggtttggg ttgcgaggca aacatgaaga ccaagaatcg
    21001 attctggaag tcggcagtgc ggctggtcat gtatctttcg ctgttccagg ccgcttgcat
    21061 cacgggttgc ctcagtaagg ttgcccgcaa catcaacccg tgcggcacga tcctgaactg
    21121 caatccaacc gaatgggatt ggatgttcca cagcggggat tatcctgact atagtgccga
    21181 cccgacgtgc gtgattcccg ggcagtgcgg cacgacgtgg cctccgactg aacctggcca
    21241 gttgggcagc gggggcagcg gcacgacgac gaccactacc ggcacgacga cgaccggcta
    21301 tctgggcgga tacaccggcg gatatggtgg cggttacggc ggcggttatg gttacggcta
    21361 ctgaccggcg ggtgccgccg gtggttcgtc ggccaggaca tgcggcagat cagtccccaa
    21421 cagcattgtg agcgggacaa ccagacggac gcgagaaccc gactctcgcg tccgtcgttc
    21481 tttgctgata gccgaccgct gaaagctgaa tgcttcttat gccgcggcta tgtggtcttc
    21541 agtcgagtca aatctcacgc tgactcgctt gctgacgccc gcctcctgca tggtgatgcc
    21601 atagagaacg tccgcgatgc ccatcgtccg cttcgaatgc gtgatgatga tgaactgcga
    21661 cttgtcgaga aactcgccga cgatgcggtt gaaccgttcg ttgttggcct catccagcgc
    21721 cgcgtccacc tcgtcgagaa tggcgaacgg tgacggccgg ctccggaaga tcgacagcaa
    21781 gagggctatg gccgtcatgg tcttctcgcc gcccgagagc agcgaaatgc tttgcagctc
    21841 cttgcccggc ggccgggcct ggatctcaat gccagcctca agaacgtcca gcggctggcc
    21901 ttcgggcggc tgttccagga tgatgtcggc cttaccgccg ccgaacagct tgcgaaacag
    21961 ttcctgaaag tgaccccgaa cggtctcgaa ggtctgcgtg aatcgctcgc ggcattcctg
    22021 attcaggtgc tcgatcagct ccgtgagctg tttctgcgag gtccgcagat cggcgcgctg
    22081 ctggccgagg aagtcggacc gcttctcaag ctcctcttgc tcggcgatgg catccaggtt
    22141 cacattgccc agccgttgga tcttgagctt gagctcctcg atttccgcct cgaccgcggc
    22201 ccagtcctgt tcctgctccc ggtgctgata gttggcgtag ctctcggcaa ggtcgacgcc
    22261 cagttcgtcg cggacgcggg caaccaggtc ttcaatccgg atgcgagcct cctgcgattc
    22321 catccgcaag aggtgcagct tttcctcgca ttcgttcagc gagcttcgtt ccaccttggt
    22381 ccgggctgcc aactgctcga tttccagccg tatctcctgt cgtcgccgtt gtcgggcaag
    22441 caggtcggcg tcccgttgtt ccttcaacga gtacagttcg gccagacggc tttcggcgct
    22501 caggatcatc cgttcggcct gggcgatccg cccggcggag tcttccgact cacgagccgc
    22561 gctgcgaacg gcttcttcgc tggcctggca ggcctcccgc gtgccgtgca gcgccccggc
    22621 catcaggctt cgacgctgca tcagctcggc gacggcaacc ctggcctggg tcatttgttc
    22681 gctcagccgc gaccgctctt cgctcagaac cgcgatctgt gcgtcaagcc ggcggattcg
    22741 ttcttctcga tccacgttct cgctttccag ccgggacagg gcttgcgagc agtgattgct
    22801 cttttcggtc gcctcggcaa tctggcgatc gattgcggcc atctcgctat ggatcagggg
    22861 ttgctcctgc atcagacgct gcacggcgtt ttcgttcgca tggcgcaggc tgtcgatctc
    22921 gacctcggcc cggagcgact cattgagcag ccgccgctgg tcttcgatcg cctgctcgat
    22981 ctgcaccatt tcggtcgagg tttcctccag ccggccggat tcggagtcga ttctctcgtc
    23041 cacttcttcg agctgcacgt ctatttcccg aagctcgctc ttgcgagata tgaggccggt
    23101 tcgaccgccg gccggtccca tttcgcaatg ggccttgagc gggtccagca actcgcccgt
    23161 cagcgtcacg tagcgataac ccggggggtt ctgtccggcc atgcgtatcg cgtcggccag
    23221 cgttcggacg atgatggtct tgcccagcag gtgccgaaca agccgctcgg cgtctttctg
    23281 gaaacggatc acgtccatcg cataggccac gaacccttcc tgctgggtga aatcgcggcc
    23341 gtcgacgaat ggcggcagac ggtcgatgca gatcgctttc acacggccgg gaaaatccgc
    23401 gaacagttcg gcatcccgga aaaacgcctc gctgtcctcg acgaccaggt attggtcata
    23461 ctcgccgagg gcgatctcga tgagcgtggc gttggccacg tcaacggcga tcaggtcggc
    23521 caccattccg cggacgtagg caaaagcctt gccggtcgcg tctcgctcct tgcggtgcag
    23581 cagctcgcgc acgccggcgt cgacgccttc aagctgctgc tcgagatcca tcagaacctg
    23641 ccgccggctg agcaggccgc tgcgatactc cttggccgcg gcgatccgac cgttcagttc
    23701 ggcacggtcg aggtcaagct gggcggctcg ctgcttgagt tcgtcgagtt gctcattgcg
    23761 cgagcgaatc tcctccgcga cctccgcagc acgagcgtcg attcctccgc gggcatccct
    23821 gagtcccgcc agctcggtct cgacctgctg ccggcgggcc tggaggcggg ccttctccgc
    23881 ttccaggtgc tggcggcgct gggcgagcgc ctgaatctcg ttgctgattt gagcgctgcg
    23941 tctcatcagg tcaataacgc cggccttgtc atcgtcgctg cgggcccgca tgccggtgag
    24001 ctgctcggcc aactgacggt cctgcacggc gaggctttcg accgccgcac gtgcggcgtt
    24061 gatccggtct tcgagctgat cgaggtccgc ctgttcggcc gcgagctgct gccgcagccg
    24121 ggccgctcgc tgtcgttcca tcgcatgtcg ttgctgggct cgcgagcgta cctcgatgag
    24181 ttcttcgatg cgctgtcgtg attgctcgat acgctcggta ttcgccgtga tctccgaggc
    24241 gttcgtcagg atctgctgct cgatctgtcg cacctcgccg tccagcacca tcaggtcgcc
    24301 gtcgagggcg gagctgcggg tttcggccga gctgatcgcc gtcctgagcc gggtgacctc
    24361 gtcctccagc cgggcttcct cctgtgccag gagggcgcgt ttctccgaca ggcgatggta
    24421 ctcggccatc gagaacgcgg cccgcttctc gcgcagcttg cggtcatatt cctggaagct
    24481 ccgggccttg ccggcctgat acttgatgct tcgcagccgt cgctcgacct cctcgacgat
    24541 gtcctccagc cgcagcaggt tctgctcgac ccgttccagc ttccgctcgg cctccttctt
    24601 gcgggccttg tagcggctga tgcccgccgc ctcttcaaag atgtgtctcc ggtcgatagg
    24661 gttcgcctgg agcaggatgt ccaccttgcc ctgttcgatg atcgagtacg ccccgacgcc
    24721 gacgccggta tccaagaaca gctcgcggat gtcccgcagc cgacactgct cattgttgat
    24781 gaggtactcg ctctcgcccg atcggtagag ccgacgggtg atggtgacct cgtcggtatc
    24841 gcgagccagc ttgcgatcgg tgttgtcaaa gagcagatcc acctgggcca tgccggcgct
    24901 ttttcgggtg cccgacccgt tgaaaatgac gtccatcatc tgcttgccgc gcaggctgcg
    24961 ggcgctctgg tcgccaagca cccacttgat ggaatcgacg acgttgctct tgccgcagcc
    25021 gttcggaccg acgatgccgg tcaccccggg caggaagtcg aagtccgtct tgtcggcgaa
    25081 gctcttgaag ccctggcagg tcaagcgttt aaggaacatg cgtgtgcgca acctccgtgc
    25141 gtccggaatt tgccgagccc tccctggccg ggtgaaggca gctcttcccg attcacaatc
    25201 ggctaatgta gccggaatca acaagaaatc caagcagatt cgaaggagtc tgtgagtttc
    25261 gcggcacgtt cagaccgggt cgagggagga acggcctgtg agtagtccag gcagtacggt
    25321 ttgggggcgg acgaccggca tgtggagcaa accggcggcg agggccgggc gggggggcaa
    25381 tcaaggctgc aggaggcaga tcggtattgc ccgccgggct tgccgaaagc tacccacggc
    25441 tcggaccggc cctgaccgtg acctttgagg cggtgcccag gagggcgtcg gccctaaggc
    25501 ggaccgcctt tgtatgggcc acaacccaaa caccctgcga tccggaaaca cacgcgcaga
    25561 aggcacaccc gtcgcgacgg aaaaggctga tgggcggccg atcgggacac gttcttaccg
    25621 gctggtgcag cccggatcag cgaggatgta ggggccgctc aggcacctct ggaacaggcc
    25681 gaagtcggac tggtcgacgt ccgcgtcgcc gtccaggtct gcgttcgcac agccgctgga
    25741 gggcggacca agacccgggc cggtgctgca ctgctggaag tgcttataat catctctgtc
    25801 gacgtcggtg tcgccgtcaa agtcggcctg ggcaagcgcc ggaggcacca ccagtagggt
    25861 gatcgacgag gccggcatct ggattgaaac ggtgcctccc gcgacggcca cgtcctgttc
    25921 gcggacgata cgggccggat cggccgcgct gtaacggtaa acctgggcac aggccgccga
    25981 cggatagctg ctgatcgaca ggctgctggt cagaatcccg ttggtcttgt tgatcaaaat
    26041 gatcgtgagc cggttgtccg atcgctgcga cgcataaatc gagacgcgtc cctggtcttc
    26101 gctgacggct cgcaccgagg tctcgccaaa cttccggccc gcgccgtcat agttgcggta
    26161 gatccggtac gcgaaggccc ccggctgggc cgattggggc ggatcccaaa gggtggccag
    26221 atcgagccct tcgcgtccga agatacccaa cacatcggct tgggcgagtg ccccgttgat
    26281 gtgctcgagc ccgccgaagt tgtattcggt gatcgccagc ttcgtaccgg gatagttctc
    26341 cgccacccac tgcttcatgc gagggatcag gcgcacataa ggaccgtcct ggacgttggc
    26401 gatccagctt tcgtccgcgt aggtgggatc ccacagcgag cgggtcgatc gcagccggag
    26461 ggcctgcgtg acggcgtcgc cggcgctgct caaagccacg ttgttgccct gcggatagta
    26521 gtgcatgtca aggtagtcca ggatccgaac accctgctgt tgctcgtacg cccgcatctg
    26581 ccggagatac cagggcacga aatgagcgtt gccgtgcgaa gcgtaatccg tgccgggacc
    26641 acagccatca agggccgagt agaaatacgc acaccaaccc cacaccacgg ggccgagcgt
    26701 gaaagcggcc ggatccgctg ccttgatagc cgacgcgtat tgaatcgttc ggtcgcggat
    26761 ctcctcatag gtggtcgggc cggggtggac atcgcgatgc gtcgagttcc agagcatggg
    26821 ctcgttgtcg aggtcgtaga accgcacgcc accctggtcg gcggtgccgt atcgcccggc
    26881 caggtgagcg atccagtcac tcacaaaagc gggtgtgatg gcgatgctgg tgtcggcggg
    26941 gtcgttgccg gtgatgtatg taccgtccgt ccggacgccg ttgccgcaat ccggccgcca
    27001 gggatccgtt ttctgctggg ggccatactt ggccacgctg aagccgcagg cataagcccg
    27061 atccttcgga gtccagccga tcagcggtac ggttatcaac gaatccgtgc cggtggcaag
    27121 gttctgctcg acgaagcgat cggcgttcga gccgttgggc aattgaccgg gattggggtt
    27181 gctgtcgggg atattctcaa aataccagtc gctcgcgcgg ttggatgtat ccaactgcca
    27241 gttgtaccgc gtggtcgcgt tcccgcccca gcgattgata ggcaggctca gttcagccgc
    27301 catctgggcc ccggcgaaat tccagccata gatcaacggg ctgatggcgt gacggtcggc
    27361 ggccacatcc acactcaggg cggggcccgg cccgggcggg ggcggccccg ccgcgacaag
    27421 ctcgatgtca tccaggtagt acaccggctg ggcagcccct cggcggtcct gccaggcaaa
    27481 accgctgaag gccggaaacg caaaagagga aatcgcgatc tccacgttcg tccaggtacc
    27541 ggccgtggtg gcaggaatgc cgactgcggt gccggcatgg ccggccgagt cgaagggaag
    27601 cacctgtatc aagtgcccgc cggttgtgcc gccgtggatc cagaaacgaa tggcgctgta
    27661 atcagtgagc agaacgttgg tgttgttgtg cagataaagc ccggcccaag ccgccgtgta
    27721 ggtgacggcg atcgacttgc cgccgtctcc gtgcacggga ctcgcgttgt tgaaatccct
    27781 tgttgtgttc cacgaccagt cctgccagcc gcttcccaga gcatcgatgt agatcgcctg
    27841 gttctgctga cccgaagcgg cggctggaca aatgaaaacg gcgaggatga acaaggcggc
    27901 ccgccggaag ttctccaaca tcttcatact cctcagatcc ccttatcctg cctcccgatg
    27961 gccgtccgac atgccgaaag cgcatccaaa gcccgccgca gccgaagaga cttgccggtt
    28021 cttgaagctt cgccgacagg tctccgatgc cggcactggc tcggactaca ccccgcgact
    28081 ctggcgcata cgtggcgtac gcaactcgct caccgagcgt cccaagggct accgttcgtc
    28141 gtcgcaggcc ttgtcggccg ggatccccgg tccacttgca caggcctcga atgccaggag
    28201 gtcttggcca tcgacgtcct gatcggcgtc ccgatcgaga aaaccacaac cgcgaggcac
    28261 aggtgcggcg gacaagcaca cctgcacgag accgaagtcg ttgtggtcca cgtcgccgtc
    28321 attgtccatg tccgcgaacg gatcgttgtg cgtgcagttg gggtctgcgg cacagtcggg
    28381 atcggcgcag tcgaccagcc catcgagatc ttcaccgtcg aagccgtcgt cacagacggt
    28441 ctccagggga caccagacgg tcgtaccggg gcaatccgag tcgagacagt cgaccaatcc
    28501 gtcatcgttg tcgtccaccc tgttgttaca gatctcgcaa tggtactggt cgtggcatgg
    28561 ttggaaccgc ccgacgctga tgctgtcgat ccagaaaatg gtcgtgccgt cgagggtcca
    28621 tggtgtaccg tcccgttcgc cgctgccgcc aagctggtac gtgagcgctc ggaacaggtc
    28681 ccgaggcggc accggcgcgg ggtcgcccga catgaacacg ttgaaactca tccagccgtt
    28741 cgcgtcgaca aggggaccgt cggtgtccag gttggctccg gaaccggccc attgatccca
    28801 cgaccagcct atgctccgga atacccagtt gctccacccg aacgcgcccc acacgtccat
    28861 tggtgagctc gggtcgactt tcaccttcgc gcggaaataa ctataagcac tgaggtccgc
    28921 gcccgaaggg atgttctcgc cgcccgggaa gacgtcacgg gcaaccagcg tctgccattc
    28981 cgcgggcggg ctgttggggt cccaagcgac ggtcaccttc agagaacccg acgtggggct
    29041 gttgtcggcg tccaccgtcg gatcccactc caaggtggta ggcggcccgg tctcggggtt
    29101 ctgttggaac cagatggccg cctgggtcgt cgggtcaacc acctcgtcgg ccgtgtcaaa
    29161 gttgttgatg gtgtagaagc cctgcggttg aggcggcggc gggcttaacc gggcattgtc
    29221 gagatggtaa acaaaaccgg cggtgtaacc ccaattgttc aagcacatga caagctggaa
    29281 ccacgaaatt gccgtccgat ggtcgctgat ggtctgtttg tacgccgagt agttccagct
    29341 cagtgtggtt gtccagtcgc cgtttcggct gtcccagtta ccggctccgc cacagtcgcc
    29401 ccaaatactg gcggtgccat catcgaaacc cgcgttgacg atgaaatgaa tcgtgcaacc
    29461 gggccattgt acggcgggat ccggcgtcca ctcgcttgcc agccgggtca ggtccaccga
    29521 gaaaacgtca ttggccagga aggcgtcgat tcgtgcggca ctggcggcca tgtcgagaac
    29581 aggaccgttt ctccagccgg cttgcgtgcc ggtgagtttg aagctgtaat cgtgcagcgt
    29641 ggccccaacc gcgtcgtagt catgggtgac ctgagggggt tcccagaaat accaaccgtc
    29701 cctgcgctgt tcccagtcgc cgagcagcgg gagtcccggg aagaaatgcg catcatccag
    29761 atagtaggaa ccggtctggg tataggctgt atcactgtag ttcgtgacga aaatcagttc
    29821 cagccattcg aggttgttcc gattgatggt cgggagaacg tggcgataat cccatgatgc
    29881 cgtggtggtg ttgttgccgt tggcgaagtt ccacgaggcc ctccagccaa gatcggtgta
    29941 gataccccag gcagttcccg aattggatcc cgcgttgatg atcaattgga ccccgttggt
    30001 ccaggtttcc gtgccggccc attcggccgc tatgcgagtc acgtcgacgg cgaactcata
    30061 gttgctgaag aaggcgtccc gcaactcctg ggtattgagc gatatgtaca cagcccgctg
    30121 ccacccgcct gccggtacgg ttaccttgag ggacgaagca ttccgtgtaa cccccgtcgt
    30181 actgaacgcg accgtggtgc ccggcggagc tgtcggagca atggcccacc cgtccatcac
    30241 gccttcccaa tcaccgatcg tctgggcgta agccgctgag gcccaaacgc acaccaaaac
    30301 cagacccgca caacatgtaa actgcttgcg catcgtactc ctcctttcac ggaccgacgt
    30361 gctacccggg ctatacttcg atgatctcag cagacagcct caggaaaacg tccgtaacat
    30421 ggaaacaggc gcgctgacag ctaaccctgc cgcgaccacc tgtgagatca tcagaatcac
    30481 cacctcaaac accagccgca acccaagcac gttgaagcca ggatgtcatg ttgatgactg
    30541 gtgtagtggc ccggtgcctc gacctccggc gaatcagaat tgcagccgcc cttgtattga
    30601 cctgagcgtg cgagcctgtt gggcaggtgc gcacgttgat gataggttac ctgaaggcgg
    30661 aaagcatgtc aacggttcgc gaaggcattg ttgaaaaggg ggccgtgacg gtccaggcgg
    30721 ctctttcaga atcccgagag gttccaaggc ggctttcggc cgcaacccaa gcgccctgtg
    30781 acccaaaaac atacgcacaa atcgcacatc agccgcgagc gaacaactcg atggtcctct
    30841 cacgaacgaa gactcgtcac cgatcctgtc ggcccgaatc atagcggtat cgaggcacgc
    30901 gcgttatcgt atagcacccg atcggacacc gggtcactgg gaggtaccaa ggcatgtctt
    30961 cgaaaccagg tggaaataga aaccaattca tcatcggcgt catcgtcatt ctgctgcttg
    31021 gtgccggctt tgttgtctgg caacgttccg gcgtcagatc cgaaaaaccg attgcagacc
    31081 agccaggagg cacaacggtg cctgaggtgg caatctatca gaagctctgc aagggcaagg
    31141 agctggccgg cgagggacga cacgccgagg cccttgccgt gtttgaggag gttatccgcg
    31201 aatccaccaa cgcctcatac aggtgggacg ccgacatcca agccgcgctg gccatggcga
    31261 acctcaagca gattcccgat gctctcaaac gcctggatcg gatcatcgcc gagtgtccgc
    31321 ttccagaaga tctgcccaac gcgcagatgg caaaggccga agtcttcagt ctggacggcc
    31381 agggcgaggt tgccattcgg atcctggatg aactggtccg gacgcacacg gacatctggc
    31441 cgaggatctg tgaggaagcg ctcgtcgcca aagccgcggc acaccagcgt ttcgggcaga
    31501 tcggtctgac gcgagtcact ctggaccgta tcattctgga ttaccctggt tcggaggaca
    31561 cccagcgtag ctgggccgaa aagcaggtga aatccctgac ggacaagcac caggccgctc
    31621 aggccgaacg cgtcgggaag atgacggccg agaatgccgt tctgcttgac aagatcggtg
    31681 ccggcgagac cacgctcacg gcctctcgcc cttacgtggt gacggatttg cttgcggtgg
    31741 ccgagggggc caccctgcga atggaacccg gcgtcagaat ccacttcggc gttcgtggcg
    31801 gcatcgttgt caaaggtcgc atcgaggcaa cgggcacgtc cgagaggcca atcatcatgg
    31861 aacccttgga tggtgatccg gatcgcgact ggtggttcgg gatccggtat cagcctgccg
    31921 aagccggaag cacctgtcgc ttcagtcact gccgaatggc tggcgcggag accgccctgg
    31981 aaaccatggg cggagccgca gagctcagcg attgcgtgtt cgaccgctgc ggccgggtga
    32041 gcgtcaaggt cggcaagggc ggtcgcctcg ccatgcgagc ctgcaagatt gaacaggccc
    32101 ggcgcgtcgg ggtcgaggcc gaaagctcat ccgtactcgt gatggacgaa tgccaaatca
    32161 ccgccgcgac cagtcatggc ctgctgctcc agggcacagg caaagagacg cggatacacc
    32221 gcacaagcat caacgcctgc ggacgcaacg gtatctttct tcggaatcgc tgctcacccg
    32281 tcatcagcga gtgccggctt cagagcaatc ttgtcaacgg cattgaagcc atcgacgagg
    32341 ccagtcccat cgtcacggcg acggcatgca aagaaaacgg caagcacggc atgctcgtaa
    32401 aagacaaatc tcagctttcc gtagcgagtt gcgcattcgt gggcaacaaa cacagcggct
    32461 tcgtggccga gggacgctgc tccggccgaa tcacgtccaa tcgcatggac ggcaatgagg
    32521 aacacggcct ggtgcttcgc ctggactgcg atccggaggt gactctcaat tcgttcgccg
    32581 gaaaccgtcg actgggggtc ttgctgcaaa acgctcaacc ggccgtcttc aagaataatg
    32641 ccttcgagaa gaacggcacg gccgccctgc gaaacgaagg accgggcgga atacaggctg
    32701 ggggaaactg gtggggaacg gctgatgaaa agggcgtcaa agcagtcatc caggatcaga
    32761 cggtcaacgg cgagtgggga gcagtcgatt atgctccctg gctgtcgtcc ccgcctgttt
    32821 cgggaatacc gaaggtcgcg gactgagaac cgcttgctcc ggcatcatcg cgacgatccc
    32881 gggacggcct acggcccagg aaatcagacc cggcaaagga agggggaacg cgaccatccg
    32941 gccgtcgcgc cggcccgccg aaactcgctc acacacaggg gaaccctgcc gattccggtc
    33001 ttgcgagagc cggccccggc ttccgcagtt cggcggtttc ctcgccaacc caagcaaccg
    33061 tctattgggg agcgcgtgct cgctgactgt agtctcttcc ggtcaggttc tggagagccc
    33121 tggcggcatg cctccgcacc gtcgggctct ggtcattgag cattctctca agatccgcaa
    33181 ccccggtgcg gctcttctgc cgcgccatgc cgttggctgc ggccagccgg acgccgctgt
    33241 cggcatcgcg cagggccttg gacaggatgg ccgccatctt cgggtcgtcg caggtgcccg
    33301 cggatcgaac cgccattcgt cgaacctcga catccgcgtt ggccatcagg ctctcgatcg
    33361 tcttcgcggc cgcgttccga tccacgctga gcagcacgca caaagcggcc tcggaaagcc
    33421 gaaggtcatc caattcaaga accacttggt cgagcaccgg cacactcttg ctgccggcgc
    33481 ttgtaagctt cgcggcggcg cgttcacgga tgatcggatt ctcactcaac aagagctcgg
    33541 tcgcgattct tcgaatctgg gcctcgctca agtccggaac gtcaaggaaa tcctccgcag
    33601 atttcgcgaa gatcagccac cccaggaaga gcacgacgac ggctccgccc gtgcccatga
    33661 cgacccagcg ggccggcccc ttgccatccc ggaaggatgt cgccacgttt tgccacagcg
    33721 cggaaatgaa ctctctcacg gtcacacctc cctgccgaca cacaccggaa aagggtgttt
    33781 tcgcatcccg cagcacacac ctcgatgcct gcgtgagctc cttaatccag ataatgctcc
    33841 tgcttgatcg ccgcggcggc gatctgcttg ggagtaagtc gctcgacatg tccgtcagcg
    33901 aacatcaggt tcgacttgcc caggtgcctc tgaaacccct tcaggaaagg atagtttcgg
    33961 atatacaccg ggtcggcaat gtactggagg tcgtccttga agcgaaaaca ctcgctggtc
    34021 gactcgtccc tcttgctgtt ggcctcaacc atcaggatgg accagtaagg tcgcttgtac
    34081 gacgtgatct tccgggcgcg gtatcccgtg gtcccgtccc gcaacttgta atgctccagc
    34141 gtgtcgcacg cgctgcgata ggtaatgtcc atgctgaatc tgttgggctc gttgccctgc
    34201 gacatgtaca agacaccttt ctcgcggacg accgccatcc tggcaggatc ttcatctatc
    34261 gcgcacttgt agatcgcgta ttcgtttccg agattcttgg caagctgcgt gatccaccaa
    34321 tggcgaccag actgcgcggc ctcgtaccag ccgaagtacg gcagccgatc gtcgttggtt
    34381 tgcgagtaca tcacggtggc gttgaagatc tgcttcaact gcgtactgca aaccaccgcc
    34441 ctgctcagcg cccgagcgtt gcgcaggctg ggcagcagta tcgacatgag caaagccatg
    34501 atggcgacga cgaccaagac ctcgatcaac gtgaaagcct ttttcgatct gccgggaatg
    34561 caatggacgt acatgagtgt tccctcttca attgaccgga tctgcaaccg cgactccaca
    34621 tcgaactgcc ggcgcagctc gagacactcg cgaagaactc tcattcaaca acacgccgca
    34681 tcgcggatac gactctcgac catgcactat tagtctaacc cctggagttg tatcaggcaa
    34741 gtccctagta agacttggga aattgctggg ccgcctgcaa atattgccta tgcttatcgt
    34801 tttcctgaac cgctgtcggg cgctcagtgt ctcgggtcgg cagatcttga cgccgctgtg
    34861 ggttgaatcg gccgaaaggc gtagagcacg ccatcgaaag cgggaaacaa taacatcggt
    34921 cggccttcgg cctttatcac cgcaggcgag gcccagagtg agtcccccat gtacgcgtgt
    34981 gaccatagaa gggggccgcc ctgataccgc cagagcatct gtcccctgcc ggcttcgaac
    35041 aagtagaggt gttcgtccca tgacatcgcc agaacgacgg gtgtttgccc tattctcgcc
    35101 aatgcacaac ttgaataggc gtaattccca gtgaggacct gccactgtaa cgttccgtca
    35161 tgcgcgtccc atttgtagac acttcggtca tatgaaccgg ccacgaccgc cgcccgggga
    35221 ccgccatcgc tcggctcttt cagccatgcc ggggtggcat caacccaata gcccgtctca
    35281 gcccgccatc ttctggcacc cgatgaggca ctcaaaccga acacgctgtt gagccgactg
    35341 ccgatgcaaa cctgggccaa cccttcgacc agcccgatgg aaggagatga atgggattcc
    35401 tcattcagca ccgccgtcca caaaacggca ccgtccgcca gagcgagcgc atggaccgtc
    35461 ccatctccgc aggcgacaaa aaccgtttcc acaccatgta ctctggcgaa tgcaggtgaa
    35521 ctgaccggca cgctcccgag ccgccgacgc catttgacct cgccgttggc ggccgaaatg
    35581 gccaggcact cacctgattg cacgttctgc gtcgcagagc gatctgcgtt ccatacgccc
    35641 gcgagcaaga gaacctcacc acgattgtcc acaacaaccg tcggcgaggc catcacgctt
    35701 cgggcggcgg tgaatgacca cgacgctgtt tcacactgcc agatacggct gccgtcgacg
    35761 gcatccagtc cgtagatcga tcggttgagt gaggcagcga acaccatcgc ccgtccgccg
    35821 acgactccca gtgcaggcgc cgcaaacacg ggcgcaccag ggcgaaactc ccacgccttc
    35881 tcgccggtac gggcatccac cgcatagacg tttcgatcct gggaacccaa aaccaccaga
    35941 ggccgacccg cgatcgtccc cgacgcagga gatgaccaga ccgcaaagcc cggctgatac
    36001 gtccatgccc gggttgtcgg ccgaaatcgc cacgcgagtt ccatctccgc cgaacccaga
    36061 tcgacgtctg atcgcccgct gtgaaggggg tcatggcaga actgaggcca gcttccctcc
    36121 tgattccacg ccgcatacaa caggggctgg tcgaactgcg gcaattcctc ggccaggagc
    36181 ggtttctcga cggggactgc cttctgccga caggaggccg aagaaagcaa caggcagcag
    36241 gccaacgtga atcgatgtcg tttgatcatg ctcgagctgc cttggcgggt tccacgggct
    36301 ccggggaaga cctctgatcg ctcgccgctt cttcgacgtc gagcgccccg gacgggcacg
    36361 cgtcgataca gcgacagcag gccaggcagg cagagtcatc cacatgaccc gcctcgatcg
    36421 cgtccgtcgg gcagacctgc cggcaggcgt tgcacttcgt gcagcggctt ggctccaacc
    36481 gcacccgcca accgctcacg cgcgtcacca ggctgagcaa agccccctcc gggcacaaga
    36541 atcggcacca ggccgacgaa aggataaccg ctgcggccag gacacccgcc aatagcaata
    36601 acatggtgta ctggcgatca ttggtgaaaa gcacgcagaa agcggcctgc atgtgaatgc
    36661 tcaggatggt tccccccacg aagaaggcaa gcgacgcata acgcaccctc cgcaggccga
    36721 cctcccattg acgatccgcc acactgaagg tgatcaggat cgaaaggacc atcagatatg
    36781 cggccggaat gccctgcagc agcggctcgt tgtgcggtcg aatgaaagcg tatgcagcca
    36841 gggttccgag caggagcgag cacgccatga caaagcgtac ccatagtacg cctctggaac
    36901 tcccttcgcg gcagaggggc tcgcctaact tgcccaccag ctcctgaaca aagccgattg
    36961 ggcagaccca tccgcagaat gcccttcccc atgccattcc aaacgcaagc accggcacga
    37021 ctagcatcat cgactgaaac gcccagacgt tgttgtagtt gatccaggcg ccgcctcgca
    37081 acaggtcgcg aaccatgcag gcacacggat gcagcccgaa tacgaaagcc aacaagccca
    37141 cggactggac gatccgccgc aaccactggc gtcgccaggc aggccgcagc cgcatgaaga
    37201 cggccccgac gctcagcagg gataccatga ccagaaacgg ggccaggtcc accgtccccg
    37261 ccaccgggac caacggttcg tgaaagatct tgaaaatgaa tccaagcagt gccatcgtca
    37321 cgtctccgcc ggaatcagat gcacggcctt ggcatcgaca aggacggcga ctttcatccc
    37381 cacgcggcac cgctccgcat cgctcagcgc acgcccggcg aggctgaccc atcgcatgcc
    37441 gtcggtctcc accaccatgc ggatcagggg accacgtaac gacgattcga tcagcgtacc
    37501 gggtaagatt gcgcacgaag cggtctgagg catcgcacct gcccagggct ggacccgaac
    37561 ctcttctgat cgcacacaag cgatcgctcg tccgctgatg gccgaggaaa acctcagtac
    37621 ttgcccgccc agcgacagtt caacctgtcc cgcacgcgcg tcccgaacaa cctgacattc
    37681 gaacagattc tcgcagccga gaaaccgggc caaggccgtc gtgtggggtt tccgaaagac
    37741 ctcttcggcc gggccggact gaaccagcag accggcaagc atcacggcca cttcatcgcc
    37801 aaggctccgg gcttcggcat gatcgtgcgt gacgtggatg gtggttgtcc cgaatcgccg
    37861 gtggcaattg cgcaactccg tggcaacggt ttcccgcgag accggatcca acatccccag
    37921 cggctcatcg agaaacatga gcttggggcg aatactgagg gcccgagcga gagccacgcg
    37981 ttgccgctcg ccgccgctca gactgtccgg tcggcgatcc agtaagccgc cgattcccag
    38041 catctccgcc agttcggtcg tggccggtcc gtcttgccga tccccgccgc gatgataacg
    38101 gcgggcaaag tcgatgttct gacgcacgct catgtgcgga aacaaacaac aatcctggta
    38161 tacgaacccg atcccgcgcg actccgacgg cagatcggta accaggcgat caccgatcca
    38221 gattcggcct tccgacggct gctccagccc cgcgattgtc tccagcagca tcgtcttgcc
    38281 ggctccgctt ggccccacga gtaccaggta cgtccccgcc ggaacctccc agcggtccac
    38341 gcatagacgg aaatcgccgg cctttcgacg gagcccttca acgcgcagca ttggcagctc
    38401 ctatcgccct gccgagcaga cgcggcatgg cccgaatgat ccacattccc acgatgaccc
    38461 ccagcgataa cagcaatacc agcacggcaa ccgactgcga ttccgattgg cggaattcaa
    38521 tgaacctttc gtaagccagc acgggaatcg ttcgcggccg gttggccaga accctcagcg
    38581 aaccgaactc cgccatggcc ctgaaccacg tcaggatggt accgatcagg ataccacgaa
    38641 cggacatcgg caaggtcacc cgcaagaagg ccgaagccgt tgaggccccc aacgtccgag
    38701 ccaccttctc gtaacgaacg tccaccgcct caaaagcaac catgctggcg cggatcagga
    38761 aaggcgaacc aacgaagacc tgagccgcga tgatgcccca ctcactgtcg aagaactgca
    38821 ggccgaaatg ctgatcaaga aaccggccca gcggcgtttt ggggcctaag aaaaacagca
    38881 gggtgatgcc cgcaatcggg ggcggcagaa cgatcggcag atcgatcaga ctatcgatca
    38941 gcgcacgccc cggaaagcag cgccgaacca tcgcataagc cagcggcaca ccgaacagca
    39001 tgaccaccgc agcggcgatc gccgaggttc tgaggcttac tgccagggcg gagaggacat
    39061 ctgcttcttt cagccgggcg tgaatgtcct gagcggaagt cagggtgcag aaatagatga
    39121 tcggcagcaa gagaagcagc agaaacatca tgcccgccaa atgacacaag ggaacgaacc
    39181 gctcccgccc gtgatgccac gcccggaccg ccggcgggag ggtcgtgtag aacagggcgg
    39241 caagcaggaa cgccgaatac aggtaggcgg gcataaaccg ctgcgatagc aggaagagat
    39301 acaggtaccc caccagccag ggatgaccga gcaagcctgc gtaggcaacc agagaaagca
    39361 cgagcaacaa agcgttttcc ggcatgatcc gcatcagggg ccagagaagc cacacgataa
    39421 cggcatagcc gaccgcatac gcgatcacgc gcggccagtt cagcgaccgg tggctcagca
    39481 ggacggaccc gagaacaacc agattggcca caaacagggc gagattgatc tgcggcctga
    39541 gcgatccgcc aggctggtcc gcgctcccgg tgatacgggc ccatgcagaa ccgaccagca
    39601 agtgcactag ggcgaggatt gcggcgccga ccagcatgga gatgattcgc tgtcgcggca
    39661 gaggaatcca attggaccgc ggacggccga ccgaaatgac tgacggcacc actggacccg
    39721 atctctcatc ggacattgcc gtctccgaaa ctgccggcag caggcaacca gcggctttcg
    39781 gcgtccaccg ccgaccgcag actgtcggga gcctgatcag aatcgcaggt ccaaggccgc
    39841 tcgacaaccc gcatgcgatc tttgatcaac aagcttttcc cctcggggtc acggccgggc
    39901 cgggaaatgc gaagaacatt gattgcggac acgccacaac cgagcatggc tcaccttaat
    39961 tcaagcgtga tcggctccga tcctttgatt tctttgcctc gtacggcaag atcttccagc
    40021 ctcgccatac ccctgcctcc ggggccgacg accgtcacgt catatcttac ctgtgggctg
    40081 gtgtttcgcc cctgcgctcc cggcaggatg acacgatcag tcagcgtcag cgacgcaacg
    40141 cctgcaccat cggtcctggc ttccaccacc gtaaggtcgc cactgccatt ccttggtttg
    40201 gctcgtagca ccgcatcggc tacagcttgt cggccgtcgg cctcaatcac cttgaatcgc
    40261 ctggtccact ggaccgtcag ctctccgctt tcgcgattga acacgatctt ggcaggatcg
    40321 aagacgcagt ctatcagcac gacctgggct gattccccgg tcaaaagaac gtcacccaat
    40381 tgtgagtcaa ataccgtgtc tctcagcacc aaaggcgccc cacctgatcc ctggacgatc
    40441 agggcgtagt tcccgctgca caacagttcg cttcgctcga tgatgatccg gccgccgtcg
    40501 acgttgacgc aactgagggc gctaccgtcg cagtaagcga aagaggagtc tcggatgacg
    40561 gcttgagccg actggcgaag acattggctg gtactgcccg agaggtagct gatccgagcg
    40621 tgatccatca gcaacgagcc atcgacgaac aatgcgtaac ctcgcgagca ggcggtgtta
    40681 ctcaacacct gactgaccgt ccgaagggtg gagtggtcga gccgcaggac gccgccggcg
    40741 cgaacctcga ttcgcaggtc gccgcacacc tgggtagcca tctcgaggca ctcccccgat
    40801 gccggatcgc cgggcttgcc gagctgcaac tcgccttcga tgatcagcgt ggcacggctg
    40861 accgcggttc gtgtctgcga atcgtagctg aataccaagg ggttgttggc ctgccgggca
    40921 atcgacgcca acgtgttgcc cggaccaacg acatggagca gtttcggtgg aaatggcgat
    40981 ggcccggcgc tcatcgtggc ttcccgctgc agccacaccg tcaccgcgat gatcgccgcc
    41041 agactggcca ggatgatgct gagcaggcgc atatcgaact caagtccgca ggaaaggttt
    41101 ccagcccggc attcgccatt cgatcttgcc cggccgtcca ccgagctgca ctctgctatc
    41161 gtaccgaagc cttccggttg cgcccaccct cttttcgact ttcccggtca cgcgggctga
    41221 agctgttctc tcttgggact tggaagggac tttacggccc tgtcctttca gcggggcgca
    41281 agccatcgtt tgggatcgtc cccaagcgag ccggtctgca gcacctctcc ggtgattcgt
    41341 tttccccggg caaagcctgc cgtggtttcg cttgacagtc cggcgccggc ggacgaacat
    41401 cctgacaggc ccggcagatc cggcgccgcg gaggatacgc aactcgtatc cgggctgagt
    41461 cgcccctgcc acggcgaatc atacccgccg gtcgcgagcc gcgatcagga ccccgtcctt
    41521 tcggtagccc catcgggagg taatctcatg tcttgggttt cagcttttcg tagccgagat
    41581 gtaatccgga atgccgtctt actggttatt tgcctgtggg ccggacttgc ggccggggac
    41641 gagcctcagg cgaccacaac ccgtcccgca agcgatgaac cggatctctg gagccttgcg
    41701 gaccagcacg caggcaccct gcggatatct acgctcttca ctgcacgtga tgtccaaagg
    41761 catctgtcgg atgccgccgg catggagaaa gccatcgcct ggtgccggca aaccggaatc
    41821 acgcatgtct acttggaaac cttccgcgac ggatataccg cggagcgcgc ggccctgctt
    41881 gccgcgaaac aacggttccg gcaacaggga tttctggtca gcgggtgcgt gaccaccact
    41941 ggcatcggac gtaagagcgt gaatggctgg atgtttccct gctttaccga acaggccggc
    42001 cttgacaaac tcacgcagat cttcgaatac gctgcgggac tcttcgatga gatcatgatc
    42061 gacgatttcc tggccacccg atgcgaatgt gaggactgtg ttcgcgcccg tggcgaccgg
    42121 tcgtgggacc gctttcgctg cgacctgatg gttgacgtct cacgacaacg cattctcgaa
    42181 ccggcccgca gggtcaatcc cgccgtcagc ctcatc
//
LOCUS       DAOTPW010000010        42074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1019_length_42074_cov_5.814631, whole genome shotgun sequence.
ACCESSION   DAOTPW010000010 DAOTPW010000000
VERSION     DAOTPW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 42074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42074
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1019_length_42074_cov_5.814631"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="P5316_01635"
     CDS             complement(<1..852)
                     /locus_tag="P5316_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR83686.1"
                     /translation="MHFHHHVITLTAMDEEKQLLTQHDDGGRNRKVPPRYRAVAEALR
                     RQMLDGHLAAGVKLPALQDLAEQFKVSTHTVRCAIRILEREGCAYRVPAVGAFVRPYG
                     TAPVSKQTVVALVTYDIGGAFELGIARGIERACQDRGWELQIYDSRSDPKAEVRSLER
                     LERNTVARGAIILPTGNDDNIEALFKLKLGGMPMVLLDRSIPGLKVDTIESEHEKGAY
                     QATQYLLDRGHERVYMLTEPPSVSSIAARIRGYELALRNRGINPGPQWRVEVDPEMSA
                     RGIQEDHR"
     gene            1377..3512
                     /locus_tag="P5316_01640"
     CDS             1377..3512
                     /locus_tag="P5316_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83687.1"
                     /translation="MRKLACVAAMLVWVGIAQAAVDWEGDNNGVGDGWDFNNALNWDD
                     RVNPAGSDWNIHRRNYGYYVPFKAWDIHVTSDIATRGATMWDRVDYPVTVTVNENITL
                     TMTGELVLQRAQNVVVKPGASLIMGPSFDSADQQINLGSTGGAYMKVESGGRAAAGVL
                     RHDNGSVLDLYGTFRASQISRIDAWEGYAFNVYGGGLVWVEDMTGSETAWYKDGKITQ
                     YLCSTVQLQGDRTSFFENYVQKGEAGEWAVDYGVTRSGYTTIRLQPADTCDFDVSPAS
                     VNTLPGADKLATQTHELTVSNTAAAGGADLNYTVVEVDRNGVVTDVPWLSVDKPTGGP
                     VAPGGTDIVTVTVDYAKLEVGVNVARLRLTADCNPCFGRDVEIIAPFIMIYNGDVDPE
                     APNAGGPGMTFTEREGEADNYRNTSPPALAVEPNALAQDGWELKIADGPDYKLKLRTR
                     PSQNIGSSTGATIVGRVRTTFKFGNNIEGENLSIWHDGGGSLGCHWSGAGDITGQGQG
                     IAGYLKETARGDNHTFADQTLDWYAGYHIIRACMGEVNGSRVIRIYFDENPAPVIEIL
                     NASSKASATDSIGFGTGSTLGQQVIYFDWVTGTNAGMFAPGEEVALIGRNLCLGPGCY
                     ESPCNTEVFADVDGDGDVDQADFAVFQLCYTGMDGGVLTDPDYCQCFDRRNNDNSPGQ
                     DNDVDSFDLAAFEDCASGPGVPANPACDD"
     gene            3568..5706
                     /locus_tag="P5316_01645"
     CDS             3568..5706
                     /locus_tag="P5316_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83688.1"
                     /translation="MVRAKIRLIAVVAVLGMAITVPAAEHWRGAGWGGDGTSWHDVLN
                     WDDSIPSATTWDVNKTGEQGTPGNHNIRITANGTCGSGTIYDQPGYTVNMTLEENRTM
                     TNSGALNYYAAGLFTVKTGAFYDMRNGQLGLESSGLTVAIEPNGRLFCSTLRHNNGAK
                     VDVYGLLEAWTVSRIAADSGYRLAVYGGGEFLVHDSVPTGGWSKDGKVTQYLCSTVTL
                     AGDHTAPADYLTKVQAGEPGTWDVSYSGGWTTIRLIESDDCDFTATPSDIESKPGAGA
                     ASSQAHEITVTNTRVGGSDVNYTITVLDRNLVPIDVADVPWLSIDKLSGGPIAPNGTD
                     IVTATVDYTKLEVGLNTVTLRFKCDCDSCEVHDVKITAPFIMVYNGDVDPDAPDSAGA
                     GLQFRLVDGQKQGFVLTSAPDTVDGFAWELPDAAGDPADPFAGPRTRWRTTPFQNLGG
                     TTGATIVGRIRVGTAVAPNSANLLIDHTSYACGLHWRGNGSAVDPVWRPGVLQELERN
                     GGVHDPPNPPSTVQLGPEEYDAPYNVAYHVFRMTGVEIVGDGGAKERVVKIYVDERPN
                     PVITVSGTKSAGADGLGFGATNALSWQHVWFDWITGTNAGAFAPGEEVSCIGRSLYLG
                     GRYDPCNEVFADADGDGDVDQADFAVFQLCYTGMDGGVLTDPDYCQCFDRRNNDNSPG
                     QDNDVDSFDLAAFEDCASGPGVPADPECDD"
     gene            5972..6787
                     /locus_tag="P5316_01650"
     CDS             5972..6787
                     /locus_tag="P5316_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR83689.1"
                     /translation="MRNIIRILAVLGMVGTAGAAEHWRGAGWGGDGTSWHDVLNWDDS
                     IPSATTWDVNKTGEQGTPGNHNIRITANGTCGSGTIYNQPGYTVNMTVENGVTMTNSG
                     ALNYYAAGLFTVKTGAFYDMRNGQLAIEHSNLTTVIEDNAHLYCSTLRHNNGTKVDVY
                     GLLEAWSVGRISADPGYSLNVYDGGEFVVYSGVPVGPWSKDGVVTMFIGSTVTLNGDH
                     SAPLDYLAKVRAGEPGTWQVEYSNGWTTIMLVPEPASLLLGLCGLAMFRRRGA"
     gene            6928..7815
                     /locus_tag="P5316_01655"
     CDS             6928..7815
                     /locus_tag="P5316_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR83690.1"
                     /translation="MVKRSVVVGMVVLACAMFCGWASAAVIVWNMGEQLNDAGKQTWA
                     MTKVGDGYGDIGAGDFNPPTNAGVNPIVQGPYTAFSRFVDSGSYGNDGFVMKNTVGTP
                     IGQTFTIDMIVKGERKLNSTGRSMGLRVGNSGATISAGVGIEPGTVFNPAFDGNALWM
                     TCRNGAYIPHGALFLKTTDAFEWRAVRITANGNGTVTLYDFLYGAAGYVGTVPEGGYW
                     TNLPAGAGGELDALGFHLNSLSGGTVTWSGFSLLGLAMNTDTALDGTTPFMLAAIPEP
                     ASLLLFALFGGLTLIRRRR"
     gene            8387..11935
                     /locus_tag="P5316_01660"
     CDS             8387..11935
                     /locus_tag="P5316_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83691.1"
                     /translation="MTVDRYARTLPVCITLILTLPAGAATLGISADGRFFTIDAVPTY
                     LLGVSYYGAQTINTPGFVTQDLDDMVARGFNWIRVWSTWDASDTGSGDCSVLSPAGIV
                     REPYMTRLKTLIAECNSRGIIVDVTMCRGSGGPNNLAEHLAYAQALATHLASYTNIYF
                     DIANECDVGDSRYVPPSEITQIVARCKQYKPGVLCTASQGATSASDLYNKYIATGCDF
                     MAPHLCRDQGCAAQTYGTVTDFIAWMSQYGRRMPVHLQEPFRRGYGSYNPVLDDFLTD
                     CAGGKNAQAAGWCFHNGWNNGNRPYRCFDMKDSEGRLFAQIDSEEWRVVNQVYGHISG
                     TALASGAPVIREVAPDPATTNVGVQYFRQMVLTQGNPWPAWSILQGPSGAQINSKGQV
                     SWTPTSADIGQTRTFEVQATNTNGSDTETWQVIVQTGPVATFEAEGAGMGHQIGRAIT
                     GGWRAVVTDTVNLFSTYGPYTTSLPTGPLVARFRLRVDNNTADNAGVCQLDVHDFDGG
                     TLLAGPVTITRQQWTQANVYQSFDLSFVNPGNNHRLEFRTYYLGAAQLDEDKIDIWYS
                     DGNYPPSVSAGADQQVSWPSVVNLDGTVTDDGLPNPPGATTVTWSKVSGPGTVTFGNA
                     GAVDTTATFSISGVYVLRLSATDSMYTATDDVTIYASGAGDFVISAEAFIGSGTLGDT
                     QNAWQLVHGRCDSAGNLHVVWYKNGAIAYRVRLAGGTWGAEETVPDSSGINLDGPRLA
                     VTPGGDVHVFWNPPAWNPLYYSIRTSGGWGARQTLIYNANGDHASTPVPVFLADGRLF
                     LIWLRWDGTDNWYYRIKTGGTWSSDQPVSPHMYDGYAVVTDGSNNVYAAGRVLGNPHR
                     AGYQVWNGTWGAVQTPATDVNQWVGGTHIAYRSGSPLALTFAGYNESGGVWYIEGVYS
                     VLVGQPSVRLTQATRCGTSVRCEDIDPKVVYDSQGNHYYVWTDGDQLRYAIRKADGTW
                     YAKDQNFPVGGGKQYFPDITAFGDTVHVLWEDSRGGMYHATIMPPGGNSAPQVNAGPD
                     QTITLPAGATLDGTVTDDGLPNPPGQVTVTWSKQSGPGEVIFGDAHAVDTTASFSQAG
                     SYVLRLSATDSQLTGEDDVTIVVNAPDIPDFDGDNDVDQSDFGVLQQCCSGSAKPYAP
                     GCEGADLDDDGDVDQSDFDAFLTCLGGPGQPAQCGP"
     gene            12095..13594
                     /locus_tag="P5316_01665"
     CDS             12095..13594
                     /locus_tag="P5316_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR83692.1"
                     /translation="MTSVFTAAILGAMPGGIGAWGQACPKEASPYGINAHAPEGQSVS
                     RLLDEVCACGIDWIRVDFVWAWIETAQDSFNWARYDTITSAARARGLTIFATIGHTPA
                     WATDGSEGSGVPRSAADYYDVCYRAALRYQDSVRHWGMWNEPNLDGFWAGTRQQYIDM
                     ILKNGADAVHAANPAAKACGPELAHLTSGDQDWYAWLADCITQAGSKLDIVTHHVYCS
                     SGYASCTDKLEKAPFWPWDPPSVKQVLQNAGWFGKPFWLTEIGWQSGSVGEAQQATNY
                     TGFLTDWFTGKSGRGWVHKVFFYELNDTQAFPDLSWGILGPDPTYARKPSFTAYQSFI
                     TAHPAPAPLPGQAYDPEPADLAAGVSLTPQLRWTAGDCAESHSIHFGLTNPPPFVGNR
                     TTTNFAPGTLSPQTIYYWRVDEVNSAGTTTGTVWRFQTGAAPGDFDSDGDVDQEDFGH
                     FQACFSGPAQPFTPGCGNADIDDDGDVDQTDFGLFESCMAGPDLPPTCS"
     gene            13625..15031
                     /locus_tag="P5316_01670"
     CDS             13625..15031
                     /locus_tag="P5316_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRR83693.1"
                     /translation="MITWRTTMSVIALAGLLATGVVPAEPPDGTVLHSAPFDEEEFSK
                     CGSLSPRGQIVLRQNTYDFADYYLYVPAGAVPEVPLPLVITSHSTVTTADEEIGLQPD
                     PTWGCCEESPYPHTRWYELAENADNVNRHNQWFVVAAPAMSSANGGLVEPWTGELQAT
                     TDERRLLAIYDQIVHTEAAAYGFALDASRVLLTGWSGGGIPTYYAGVRHPEIFSMIVS
                     RQGNVDEDMFDTAAVPLNTNLLVAILNGLTDTVVPRSKHEAAQDWLIGNGYPRVFVSP
                     ADPHIRDLPDAEFPDVPSNHYCHSLVTFDTFMDAMPPVGPVIAAVTPSPEIVTPGSQY
                     QRQLRLMQGTPPVEWSVLQGPTGIQVNAAGLVSGWASGVADLGETFSFEVQADNSMGS
                     DVVTWQVRVLSACDFDKDGDVDQADFGHLQGCLSGHGQTCSPECIDADLSSDGDVDEV
                     DFGLFQACLAGPDCTPGC"
     gene            15046..16389
                     /locus_tag="P5316_01675"
     CDS             15046..16389
                     /locus_tag="P5316_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83694.1"
                     /translation="MRQLLLPSFIVCLAAGTVALADPVNPAASTPAGETLPPGYVRGD
                     VVSYSYVPNRYWTRAEAFGICPYLFEYGLIKWESDAPLETFHAENIRRIDMVSKWSTV
                     IGLCNRHREWQDEVVNLMIRCFRNRQLILLPARPSPNKPEKDSFKHLIALLERLWKDR
                     DKTLVSPEGDRATGRQLINNILMADLGDEGLSILKTEGLTKLNQRFDREIRNRLIDGQ
                     RPFRHIKTWYNEVHCGIGCYAANQEDLAKGKVKWPANSEFIGVDVYHYWSFDHTPFDP
                     DDPGVPRDRVLQHAIAWQNVITKYYGPDFRVTAGDAWDHKHKNDTHAMLQAIDLAGAS
                     RAMMIYIGNTQYVVGSCYTTPIETMDAFYDSVKAGPWVGLSWWVFDTGGVGGGTLDYL
                     DGTLRHYTPANKKGKPYTTAELENYRKRFIASRMRMFNDVVYNQFGYLNGPAPKQ"
     gene            16413..17861
                     /locus_tag="P5316_01680"
     CDS             16413..17861
                     /locus_tag="P5316_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83695.1"
                     /translation="MHRDYGPMWVKAVALVAVMAAGPAGFVLGAGADVRTSSRPAGER
                     AEGFPPGHVRGDVVSYSYVPIRTWSPSESIGILPYLFEYVLTTLDDRKPLEAFHAENI
                     RRIDIASQWSTAIELYNRRVSYRDEIINLMIRCRRNRQLIILWGRPSARKNDDKDTSR
                     SLTEILEKLWADRDRELVSPEGDRATGRQLINNILAVSLGDEGECGLGTQGLEKLFAG
                     FDRNVRLREMDGQRPFSHIKGWYNMLSYTALNYNGCYAASQTDVDEHKRVKLPLNTQA
                     IGVDAYHYWGHKHSPFDPADLSIPRAKVRAHSEEWQRIRTRYYPEGVQVRVCENSGDP
                     QTWTPECWSDTHALMSAIELAGAKNVMMWYIAACGQIEGQSYTTPIETMESYYEHLKA
                     GPWVGLSWWTFGGFQEGGEHGAHFIGGIEYYDRVLKHYTPQHPEGEPYSAEMLDYWHR
                     EYVALKMRMFNDVVYNQFGHLNGPRPIAQSKE"
     gene            17866..19281
                     /locus_tag="P5316_01685"
     CDS             17866..19281
                     /locus_tag="P5316_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83696.1"
                     /translation="MCGKNGCLFSAMVMVFAAVSAQTQGGVWRGSTTRPSGEKLPPGY
                     VSGDVVSYSYVPIRTWTPKEGIGILPYLFEYDVMLWSGKQRLEDFHAENVRRIDIASR
                     WSTAMPLYNRYDYYRDEIINLMIRCFRNRQLIILSATPNTRDKEKDAHTNLIAILTTL
                     WQNRDRELTNPEGDKATGRQLINNILAASLGDEGECGLKTAGLEKLFADFDHEIRFRE
                     MDGERPFRHIKGWYNMLGYAALGYDGCYAASQEDVDKHNRVKLPPNTQAIGVDVYHYW
                     FHKYSPFDPADLSIPREKVRAHSDEWQRIRTRYYPEGLEVRVCENAKDPATWIPECWN
                     DTHALLSAIELAGAKNAMMWYIGVSGQLGPAGDETTYTTPVETMQAYYDELKAGPWVA
                     LSWWVFGGSNTCHGGLEYYDRTLKHFTPRHPEGEPYSQEMLDYWHDAYVKTKMRMFHD
                     VVYNQFGHLNGPMPVTQDKKE"
     gene            19286..20692
                     /locus_tag="P5316_01690"
     CDS             19286..20692
                     /locus_tag="P5316_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83697.1"
                     /translation="MSRNERHRCLIAVLAAAAASTPSRGDVWRGSTTRPSGEKLPPGY
                     VSGDVVSHSYVPIRTWTPKEGIGILPYLFEYNLMCWSGDQRLEKYHTENVRRIDIASK
                     WSTAIQLYNRYDYYHDEIINLMIRCFRNRQLIILSARRGDRSKDREKDAHANLIAILT
                     TLWQNRDRELTNLEGDKATGRQLINNILAASLGDEGECGLKTAGLEKLFADFDHEIRF
                     REMDGERPFRHIKGWYNMLGYAALGYDGCYAASQEDVDKHNRVKLPPNTQAIGVDVYH
                     YWFHKYSPFDPADLSIPREKVRAHSDEWQRIRTRYYPEGLEVRVCENAKDPVTWIPEC
                     WNDTHALMSGIELAGAPNAMMWYIGVSSQIDNSSGAATYTTPVETMQAYYDELKAGPW
                     VALAWWTFGTDRDTHGGLEYYDKTLKHHTPRHPEGEPYSQEMLDYWHRAYVDVKKRMF
                     EDVVYGQFGHLNGPVAKP"
     gene            20741..21046
                     /locus_tag="P5316_01695"
     CDS             20741..21046
                     /locus_tag="P5316_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83698.1"
                     /translation="MRDTRVAAAQFEHRDGDQAYNPGRICDPTRKAVDDGAEIVCFHE
                     LWMPASGWIQPTRPEEAGRDHRARAEWAKHPGVDPNRQGSFDHHDGGASRDRRRTLL"
     gene            21190..22677
                     /locus_tag="P5316_01700"
     CDS             21190..22677
                     /locus_tag="P5316_01700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRR83699.1"
                     /translation="MTIYKSIIPFPRLRTASGPGLRVVMIGLAVSVTIVQGAETQQIS
                     AGVRTSRPDPYAPTTLPDRIVLTWCGDPTTTMAVTWRTSPDVRKGFAEIAPAEQGQRF
                     YQELNGNEYKKITFDNQQRTVEAQTTSFESPGPRSCYHSARFEGLSPGTRYVYRVGDG
                     VRWSEWFQFRTATREAEPFRFIYFGDLQDAVASMCSWVFRAAILSAPDAAFTLHAGDL
                     TASRGSDVEWGEWFAVGGWVNAMVPVVATPGNHDYFKTAPDGSPGAYLEPHWRAQFTL
                     PENGPAGLEESCYYIDYQGARIISLDSNVRREEQAQWLDRTLSSNPMRWVIVTCHHPA
                     YSVANHRGGGVRREWQSVFDKHDVDLVLTGHDHAYSRTGLIRRGKDDTPVEGRGEGTV
                     YVVSVAGPKMNWPIVAKGEPTGFAKREAQDTQFFQVISIDGDKLRYEAWTATGVLYDA
                     FALVKQGRGRKELIEEIPAAPARRRGQLGSASGPDRGAVLLPPEK"
     gene            22788..26138
                     /locus_tag="P5316_01705"
     CDS             22788..26138
                     /locus_tag="P5316_01705"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HRR83700.1"
                     /translation="MKKRLTLVVGSLLMLVVPAAAEPAPLAAVNSAGVERDPWLSPDG
                     YTLYFASNRSGGGFDIYQSQWDGHQWSQPKALGSHVNSDFDDIRPSLSADGRRLLFVS
                     HRGRFYRGVWICDRQPDGSWGQARYVTVDEQPAVDDAVIGRDGRTIFYKRISKNQPPT
                     DRRWDMCMKYDPRRRFWEVPRPYHGPLPENHVPGAFWRFSVKDGDIYVEAATEAEPQK
                     TDVPEVLNCLKTSSLYGREDQEYWVSEGQNLVDGLEDTSWISKEGLPINQQWVLLGLN
                     GRAKYVPGLSRIHRFRIHSGPAEEHRDQERKDAVVIFDKEKGPPARPTRLRILGGMDV
                     DSLKELAVVELDLNSGSPWHDVPLKEPTYLRYIKIEVLETAGPAADCVAFNEVEAYGA
                     GLSARRPAHRVSTDENNNILVDGEPFFPVYLYYAKAEACLADYGFNTTLETYDVAPDS
                     ARLAILDRAEDSGFMVIGHVPNVDHEFERKRARNQLLALRHHPALLGYLMSDEAGHSP
                     EIMAMDKRRSDFIHEHDPNHFTMLNDLYPTYYPRSSAFVDVFSIDPYPHIVGQPFSYQ
                     GYAVDVAYQCVQHKKPVFVVNASWGPILSPVENRLGVYLALIHGAKGISWYALGVRNE
                     DDDHYASILRCTREIRRLEPVWFAPDPKPDSALLTHSRIENPSARIDVMIKEVAGEVW
                     LLAANCEPRQATVTFSFGWGDEIVIKEVMAEQPQAWSMNRRDFHTMDAWPPSEDREPV
                     RQARPIDLTFPPYGVRIFRMKPVGKIGPLYQPVGDGTQLAMVNESAIRRTAEQAKSLR
                     EQGRLEDAKKAVDAFWSKYGKRVSVDDLSSLMDILGQKASADEILADYGRLAEEHPEA
                     ESWPKWVYKIVAALHEEKRDQEAGVWMSRLIRHRPDSLWRANAEALVNPASAREGHKP
                     WTQAVKIDTKPVIDGELTEPFWQSRVKFKNTVFLDSSKNPQETEFAVAYDDQALYFAA
                     RCVEPQPSQILKRCDKDDGSVWADDCIIIYLDPKLEYKSYYQLIFNVLGTKWDGTGSS
                     GGHANADGLNAKNVERRAVVKDDAWQIEIAVPFSDLKRPAPSPGQVWGLGVQRWRQVG
                     GALPTVWGNERGTSHDSRPEDFGFLVFR"
     gene            26429..28333
                     /locus_tag="P5316_01710"
     CDS             26429..28333
                     /locus_tag="P5316_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83701.1"
                     /translation="MREVKEVKLAGGESSANTQSGTWRGRLCRFLCRPGWPILVALIA
                     MILVSPSLRLGWVFDDDFHRLTIRGSTRCLAFSGSIWDMFRFLDGDAARTLELMDHGV
                     LPWWTVPEAKGSFWRPLTILTHWIDYHLWPDTAWLMHAQSIMWYGLLVLAAAALYRRM
                     MGAGIAAGLAMVLFAVDDAHSMPAAWLSNRNAIVSGVFGVLAILAHDRRRRQGRTGSA
                     VPAWLCTAASLLSAEAGVGTFAYLVAYTVCLDRCRWRQKVFIMLPYLTVLIVWRVAWT
                     LGGHGVQYLGLYVDPFREPAVFALSVIQRIPLLMLGQWAGLPAEAAILLPNRLMLLLQ
                     LLGVGITIVLAAVAWPLLRRDPVARFWALGMALSLVPACATFPANRLLVFAGIGAMGM
                     LAQFLLDVLRLENHSGAVGGLSVGAVADKPLRAPGRNGHPHARRLCRVVAWSFVCLHI
                     VLAPVALAVQAAFPMGFKRFNDELTVRTALDGSVEQQTLIIVNAPSVMHAMYLSVQRE
                     LAGQPVPRHTRVLAPALPAVKIRRLDKNTLSIRPEDSFIAWRFDHLFRSERRPMSLGQ
                     RVKLSDLTIDVTELTPDLRPAEALFHFAVPLEHPSLRWLHWQDGEFVPFTPPAIGETI
                     ELRPRPPSLW"
     gene            28481..29959
                     /locus_tag="P5316_01715"
     CDS             28481..29959
                     /locus_tag="P5316_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRR83702.1"
                     /translation="MPTAGDPIVDPTTEPGRFSWPTAFLVAAASGGLLAACFRPLGLH
                     FLAWIALVPWLMMFPRLSPAATWLFGIVLGLVFYRIGLDWLIKIAGPIAGATIIVLAV
                     WMGFAFRVARLLTVRFGPASMLWAVPLAFVGAEVLRCEGLPRLRFSFLAFGYSQSHNL
                     RVAQIASIGGVYFLSLLLVAVNAAVAYGLWQRRRRAWIPAVVMAAGVIVVGVISQPPS
                     FVLQDTVTVACVQGEELRYREYLDLAARAASARPTPAFIVLPEHTITEAATEDHRMVS
                     GLAELAREHDLYACVGAHITSQAGSCRYDNVAMLIGRAGIVGTQAKAVPLPFFDDGNP
                     ARSQAAFDTTYGRIGMYVCYDGLFTDHPRHLARLGATLILVPVMDPARWPAQEQWQHA
                     DMAPMRSIELRRSAVRAASSGITQIIDPRGRVIAQRTRDEGPGAVVASVPLCNRQTFF
                     MRGGHLFAAAVGWSFLLTFIVLTLWQWIRGLIYLSGRGSHKT"
     gene            30187..31242
                     /locus_tag="P5316_01720"
     CDS             30187..31242
                     /locus_tag="P5316_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRR83703.1"
                     /translation="MSRTMKVVSLIAIAVSVLGIVLVAGCKDRGAGGKAKPIEMNYSI
                     FFPDVHIVCQTAKAWAEEIEKRTDGRVKIKIHPGGTLTAADKCYDGVVNGISDIGMSC
                     FAYTRGRFKLLEGLDLPLGYPNGVTATRIATELTMKYEPAELSDVHVLYVHAHGPGIL
                     ASKKAVRTLEDMKGLKVRATGLSTKIVEALGGVPVAMQQGDTYEALQKGVVEATFCPI
                     ETLKGWKQGEAINFVTDTSCIGYTTAMFVVMNKKTWDNLAEDIQKVFTETSREWVFKQ
                     GAAWDQADQEGLAFVKGLGREIITLSPEQQERWKERVQPIIDEYVAAAEAKSLPGAAF
                     LKDIQMMIAQSAASKPE"
     gene            31251..31769
                     /locus_tag="P5316_01725"
     CDS             31251..31769
                     /locus_tag="P5316_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRR83704.1"
                     /translation="MQPIISKLSGFWTAYARSVRMLVYVLEIIAALGIITMMLLTCAE
                     VILRIFRISVTGVVDVVCVAAAVTAAAAIPYTTACKGHVAIEYFFQKLSRRGRIVVDT
                     LVRICVVLLFGFLTVECVSYGMSLKANGQVTPTLQMPDFWVPYVLAASCAVVCLVTIY
                     HLFHPGKELIKP"
     gene            31766..33082
                     /locus_tag="P5316_01730"
     CDS             31766..33082
                     /locus_tag="P5316_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRR83705.1"
                     /translation="MTPVEIGILGCIVLVLLLVSSMPVAFAMAIVGFVGFAVVVTPHA
                     AVSMATTELCDTFSKHSLTVIPLFVLMGQVAFHCGISRRLFNTAYHWFGPLPGGLAMA
                     TVGACTAFGAICGSGPATAATMSLVALPEMKRYGYSMELACGTVASGGTLGMMIPPSV
                     VFIVYGIMTEQSIGKLFIAGILPGLLTAGLFTLFIYVECIRRPGYGPAAPSTSWGAKF
                     VSLLGVCETLALFLLVIGGMFAGFFTPTEGAAIGAGGTIVIALVKRQLAAKMVWRALQ
                     ETMRTSLMVMIIVAGAMIFGKFLTVTGIPTELAAWLGGLPLPAWVIVGLIITFFIIAG
                     CFVDALALILLTVPIFRPIINGLYPEQDPMLLIWFGVIIVLVTQIGTITPPVGVCAYV
                     VGGIERDVPLQAVFKGCVPFVMALVIATVLCVAFPQICTWLPGMVR"
     gene            33102..33587
                     /locus_tag="P5316_01735"
     CDS             33102..33587
                     /locus_tag="P5316_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRR83706.1"
                     /translation="MDSGTFPVSPYQKILFCTDFSENADYAFNFAVDAAVRRPGCTLY
                     LLHVIPESDAQFWKTYIYEVDGIDEKAKQDIDEKINLDYRPRVPAGVDFEVEIRVGKD
                     YQVILEFAEERDIDVIIIGRQGRGGLQKALFGNVTEKVARKAPCAVLIIPMDFQKKHP
                     R"
     gene            33779..34024
                     /locus_tag="P5316_01740"
     CDS             33779..34024
                     /locus_tag="P5316_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83707.1"
                     /translation="MATLTRAQEAFRASNKAGLNRPVEVIIGEGNHRLAEPLVLGPED
                     SETESAPITWQAKGRVILSGGRVITGCRETTPNGNTD"
     gene            34535..36598
                     /locus_tag="P5316_01745"
     CDS             34535..36598
                     /locus_tag="P5316_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018822976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRR83708.1"
                     /translation="MGCSAGTSPGSNDARPWVIPANLLGQTDHEQFAGNDDVLGRELG
                     VRPRLLAKALTIRRTEERLLKLFAEGRVSGTVHTCIGQEWVGVAVADTLKEGDFVFSN
                     HRCHGHYLAWTNDVEGLIAEIMGRSTGVCGGRGGSQHLCKDGFFSNGIQGGIAPVAAG
                     LAMARRLDGGSNIAVVFIGDGTLGQGVVYETMNIAAKWHLPLLIVLEDNGVSQSTLQD
                     ETLSGSIKGRAHAFGIDVRSADTWNPSELIDVTAAAVSHVRRTRHPVLLHIRTYRLKA
                     HSKGDDNREIEEVQAYAEIDTLSRLLDEPSDPIRNLIEKIDRRLDEAVDLAEQASPTE
                     PVVLLDHAPAGTEPVEWRSLDFRPQRVVDAIRTALDESMAEDDRIVLMGEDVCDPYGG
                     AFKVTAGLSSKYDDRVRNTPISEAAVVGIGSGLAIAGFKPVVEIMFGDFVMLAADQII
                     NHAAKFAEMYNAQVRVPIIIRTPMGGYRGYGPTHSQSLEKHLLGVPGTRVLAVHTRYC
                     PGTLYRRLLQTIDRPTLVIENKLLYGRRADPQAPAGSVISATATDFPTIRISPESEPS
                     LTVVAYGGLVPMAETAVESLIDEDIACDMLIPTQLYPLDIEPIVESVARTGRILVIEE
                     GQGFAGFGSELIAKLAEDRRIPSLRARRVHAWPCAIPASSTAESTILPSVESILKAVF
                     QLVLG"
     gene            complement(36803..39430)
                     /locus_tag="P5316_01750"
     CDS             complement(36803..39430)
                     /locus_tag="P5316_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRR83709.1"
                     /translation="MISTNLSTGLPALDRVLKGLLPGDNLVWRVDTVDDYRVFLEPFC
                     RNAVSAGKRLVYYRFADHAPLVPADCPAEIHELQPELGFEKFISSIHEVVKSARGLGA
                     HVFDCLSDLAVDWSSDRMLSNFFVLTCPYVYDVEGIAYFPLLKNYHSPQAVATIANTT
                     QILVDIYRHRDRYYLHPIKVQQRHSPTMYMLHAWEPQHETFRPVNDSSTIAEILTSRP
                     WGSTEQGIARLGVWARAFREAEELVDPSLQKACSLEEKKHLHRRLMRMLISRDERILP
                     LAEKYLNPARILEIRRRTLGTGLIGGKSVGMLLSRAILEQSDPKWSRRLEVHDSFYIG
                     SDVFYTYLVQNGVWWLREKQRNPQNFLEGSEEARRRIITGSFSEETQKEFAEMLDYFG
                     QSPIIVRSSSLLEDAYGNAFAGKYESLFCVNQGSRDKRLADFMSAVRTIYASTMSEKA
                     LQYRAQRGLLEKDEQMSLLVQRVSGSLHGNLFYPQVAGVGLSYNPYVWHEDIDPKAGM
                     LRLVFGLGTRAVDRSDDDYTRVVALNAPGRRPEAGIDQVRRYTQRKVDALDLAANQLV
                     SNDFVDVIRQSNDIPLQIFASRDSELERMAAETGRKDLFPWILTFDHLLSETSFVSDM
                     REMVATLEKAYACPVDIEFTANFFEDSEYKINLVQCRPLQVAGGRADEAAEMPASIDP
                     KDLVIEAHGAVIGQSRAAAIDRLVYVVPSVYGHLPIPERYAVARLIGRLMHADEEQKP
                     ERIMLLGPGRWGTTTPSLGVPVSFAEINTVSVLCEIVAMREDLVPDVSLGTHFFNELV
                     ELDILYLALFPGREGNFLNEEFFEKAPNKLTRLIPSAANWAHVIRVIDTADLGGRGHI
                     QLNANNLSQRVVCYRSRTQ"
     gene            39834..40718
                     /locus_tag="P5316_01755"
     CDS             39834..40718
                     /locus_tag="P5316_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRR83710.1"
                     /translation="MFVIQEAKWLAPAVRRLVVEAPRVAAHHKPGHFVIVRVVEGGER
                     IPLTIADSDEAAGTITLVVQAVGTSTQRLAALEKGQAILDVAGPLGKPTEIKGFGHVV
                     LVGGGVGTAVIYPQAGALKAMGNRVSAVIGGRSRPYVIMEQELKAVCDAVYPCTDDGS
                     YGYHGLVTGRLGELIDDASDPVNAVITAGPVPMMRAVAEVTRPKAIHTIASLNPIMVD
                     GTGMCGGCRVMIGGRMMFACVDGPEFDAHQVNFAELADRLTAYRDYERKAQARTPTNH
                     ECKLIEAEKAIRREAPVA"
     gene            40764..>42074
                     /gene="gltA"
                     /locus_tag="P5316_01760"
     CDS             40764..>42074
                     /gene="gltA"
                     /locus_tag="P5316_01760"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRR83711.1"
                     /translation="MQLTPAERMKIPRQQMPEQKPEMRTRCFQEVNQGLALEAARMEA
                     ERCLRCKEGKCVQGCPVGVDIPGFLVALADGDLNKAADILYEANVLPGITGRVCPQET
                     QCESMCIRGKKGSPVAVGYLERFVADWARDNRPDQYVPPPPTGKRVAVVGAGPAGLTC
                     AGELARKGHGVTVFEALHRPGGVLVYGIPEFRLPKKIVEVEVDKLRKLGVEIVCNVVI
                     GRTHTIRELIEQEGFHAVFIANGAGLPVFQGIPGENLKGVYSANEYLTRVNLMGAYRD
                     DSETPVIRSTEVVVVGGGNVAMDAVRTARRLGADPATLVYRRSRAEMPARVEEVKHAE
                     EEGVRFEFLVNPVEILGDDKGWVRAVRCQRMELGEPDASGRRRPVPVKDSEFDIPCQV
                     FIEAIGTKANPLLTQSTPELKLNKWGNIEIDANNMTSIPGVFAGG"
BASE COUNT         8175 a        12190 c        13458 g         8251 t
ORIGIN      
        1 ccgatgatcc tcctggatac cgcgagccga catctccggg tccacctcca ctcgccactg
       61 cggcccgggg ttgatgccgc ggttccggag cgcaagctca taacctcgga tgcgggcggc
      121 gatcgacgac acgcttggag gctcagtcaa catgtagacg cgctcgtgtc cccgatcgag
      181 aagatactgc gtggcctggt acgccccctt ctcgtgttcc gattcgatcg tatcgacctt
      241 caagccgggg atggaccggt ccagcagaac catcggcatg ccgcccagct tgagtttgaa
      301 gagcgcctcg atattgtcat cgttacccgt cggcagaatg atcgccccgc gagcaaccgt
      361 atttcgctca aggcgttcga ggcttcgcac ctcagctttg gggtccgaac gagaatcata
      421 gatctgcagt tcccatccgc ggtcctggca agcccgttcg atacctcgtg cgatccccaa
      481 ttcgaacgcc ccgccgatgt cgtaggtgac gagggcgacg acggtctgtt tcgagacagg
      541 cgccgttccg taggggcgga caaaggctcc gacggccggc acccggtagg cgcatccttc
      601 gcgctcaaga atccgaattg cgcatcggac cgtatgggtg ctgactttga actgctcagc
      661 caagtcttgg agggcgggca gcttgactcc ggcggccaga tgcccgtcca gcatttgtcg
      721 ccgcaacgcc tctgcgacgg ctcgatagcg aggagggacc ttccggttcc gaccgccatc
      781 atcatgctgg gtaagcaatt gtttttcctc gtccatagcc gtcaatgtaa tcacgtgatg
      841 atgaaaatgc aatagtgttt ttccaagaat tcgtcgcaca tcttctaagt tattgttcta
      901 aagtgactta taaccatcct cgccgtggat caggccctga tctgaaaata atcatcacat
      961 gaggtggatt ttgcttgatt tgttgccagc ggcgcgttac gatgggccac gtgcgcaagc
     1021 cgcagttctt gcgtttggcc gtttccgtcc gggcgagggc tgtgaaggaa atgcggcgtg
     1081 atggcagcac aaacgggtgg ggcaggtctg aaggaggcag gtttctgaag cgcctggcgc
     1141 gcaatgttcg ggaaggggca ttggcggtgc tgtttgctaa ccgctgattc ccatgcccgg
     1201 ggttgctttc agttctttga agcgaaccgg ggacggtgga ggccttaagg cgggtcgctt
     1261 tcttgcgggc cgcaggccaa atgcactgtg atccggaagg atgcgcgcca gcggcacaga
     1321 acagatcttg ggccataagg ttcgaaggca tgagaaagcg agaaggagga aaagggatga
     1381 ggaagctcgc atgtgttgcg gcgatgctgg tctgggtcgg gatagcacag gcggccgtgg
     1441 actgggaagg cgacaacaac ggcgtgggcg atggttggga cttcaacaat gccctgaact
     1501 gggatgatcg cgtcaacccg gcgggcagtg actggaatat ccaccgacgg aactacggct
     1561 actacgttcc cttcaaggcc tgggacatcc acgtcacctc agatatcgca accaggggcg
     1621 ccacgatgtg ggaccgggtt gattaccctg tcaccgtcac ggtcaacgaa aacattacct
     1681 tgaccatgac gggcgagttg gtcctgcaac gggcacaaaa cgtggtcgtc aagcccggtg
     1741 ccagcctgat tatgggaccg tcgttcgatt cggccgatca gcagatcaat ttgggttcga
     1801 ctggcggtgc ctatatgaaa gtcgagtccg gcggaagagc ggcggccggc gtcttgcgtc
     1861 atgataacgg ctcggtcctt gatctgtatg gtacgttccg ggcttcgcag atctctcgca
     1921 ttgacgcctg ggagggttac gcgtttaacg tgtacggtgg cggactggtc tgggttgagg
     1981 atatgaccgg ttctgagact gcatggtaca aggatggaaa gatcacccag tacctttgtt
     2041 cgacggtcca actccagggc gatcgaacga gcttcttcga gaattacgtc cagaagggtg
     2101 aggccggcga gtgggcggtc gattacggcg tcacccgatc cggctacacc acgatccgtc
     2161 tacagccggc cgacacgtgt gatttcgacg tttcgccggc ttcggtcaac accctgccgg
     2221 gcgcagacaa gctcgcgacc cagactcatg agctgacggt cagcaatacc gcggcggcag
     2281 gcggggcaga tctcaactac acggtggttg aggtggatcg caacggcgtc gtcacggatg
     2341 tgccgtggct gagcgtggac aagccgactg gcgggccagt cgcccccggc ggcacggaca
     2401 tcgtgacggt taccgtggac tatgccaagc ttgaggtcgg cgtgaacgtc gcccggttgc
     2461 ggttgaccgc cgactgcaat ccttgttttg gccgggacgt cgagatcatc gctccgttca
     2521 tcatgatcta caacggcgac gtggatccgg aggcccccaa cgcgggcggg ccgggaatga
     2581 cgtttaccga acgcgagggc gaggcggaca actaccggaa cacatcaccg ccagccctgg
     2641 cggttgagcc taatgccttg gcccaggacg gctgggaact gaagattgcc gacgggccgg
     2701 attacaagct gaagcttcgg acgagaccgt cgcagaacat cggcagtagc actggcgcga
     2761 cgattgtggg ccgggtgcgc acgacattca aattcgggaa caacatagag ggtgagaacc
     2821 tgtccatctg gcacgacggc gggggctcgc tcggttgcca ttggagcggc gcgggcgaca
     2881 taactggtca gggccagggc atcgcgggct acctcaagga aaccgcgcgg ggtgacaacc
     2941 acaccttcgc cgaccagacg cttgattggt acgccgggta ccacatcatc cgcgcttgca
     3001 tgggggaagt gaacggctcg cgggtgatac gaatctactt tgatgagaat cccgccccgg
     3061 tgattgagat tctcaacgcc tcgagcaaag cgtcggccac ggattcaatt ggtttcggca
     3121 cgggcagcac gctgggtcag caggtcatct attttgactg ggtcacgggt accaacgcag
     3181 gcatgttcgc ccccggggag gaggtcgcgc tcatcggccg gaacctgtgc cttgggccgg
     3241 gctgctacga gagcccctgc aacaccgagg tgtttgccga tgtggatggg gacggggacg
     3301 tcgatcaggc cgattttgcc gtctttcagc tctgctacac gggtatggac ggcggagtgt
     3361 tgaccgaccc tgattactgt cagtgcttcg atcgccggaa caacgacaac tcacccggcc
     3421 aggacaacga cgtggacagt ttcgatctgg cggctttcga ggactgcgcc agtggtcctg
     3481 gagtgccggc caacccggcg tgcgatgatt gaagaggtag gcgagggatt cgaggctcga
     3541 actcgaacgt agaggaggag cgagaacatg gtcagagcaa agatccggtt gatcgccgtc
     3601 gtggcggtgt tgggaatggc catcacggtc cctgcggccg agcactggag aggtgcgggc
     3661 tggggcggtg acggcaccag ttggcacgac gtgctcaact gggatgactc gattccgtcg
     3721 gccacgacgt gggatgtcaa taagacgggt gaacagggca cccccggcaa tcacaatatc
     3781 cgcattacgg caaatggcac ctgcggcagc gggacgatct acgaccaacc cggctacacg
     3841 gtcaatatga cgttggaaga gaaccgaacg atgaccaaca gcggggcgct caactattac
     3901 gcagcgggcc tcttcaccgt caagacgggc gctttctacg acatgaggaa tggtcagctt
     3961 ggcctcgaga gcagcggcct caccgtggcc attgagccca acggtcgtct cttctgcagc
     4021 actctgcggc ataacaacgg cgccaaggtg gacgtgtatg gcttgcttga ggcctggacc
     4081 gtcagccgga tagcggcgga ttcgggttac aggctcgccg tatacggtgg aggagagttc
     4141 ctcgtccacg acagcgttcc aaccggcgga tggagcaaag acggaaaggt gacccagtac
     4201 ctctgttcaa cggtgacgct cgccggagat cacacggcac cggcagatta cctgaccaag
     4261 gtgcaggccg gcgagccggg gacctgggac gtcagctact ccggcggctg gactacgatc
     4321 cggctcattg agtcggacga ttgtgatttc acggcgactc cgagcgacat cgagtcgaag
     4381 cccggagcgg gcgctgcgag ttcgcaggcg cacgagatca cggtaacaaa cacgagagtg
     4441 ggcggttcgg acgtcaacta cacgattacc gttctggacc gcaaccttgt cccgatcgac
     4501 gtggccgacg tgccctggtt gagcatcgac aagttgagtg gcgggccgat cgcaccgaac
     4561 ggcacggaca tcgtgacggc gacagtcgac tacaccaagc tcgaggtggg actgaacacg
     4621 gtgacgttgc ggttcaagtg cgattgcgat agctgcgagg tccacgacgt caagatcacg
     4681 gcgcccttta tcatggtgta caacggcgac gtcgatccgg acgctccgga ctcggctggt
     4741 gccggcttgc aattcaggct ggtcgacggt cagaaacaag gattcgtgtt gacgtcggct
     4801 ccggataccg tggacggttt cgcttgggaa ctccccgacg ccgcgggtga cccggccgac
     4861 ccgtttgcgg gtccgcgcac gagatggcgc accaccccgt tccagaatct gggcggcacc
     4921 accggagcga ccattgtggg tcggatccgg gtgggcacgg cggtggcgcc gaactcagcc
     4981 aatctgctga tcgaccacac aagctacgcc tgcggcctgc actggcgggg gaacggcagc
     5041 gcggttgacc cggtatggcg gcccggtgtt cttcaagagc tcgagcgcaa cggcggtgtg
     5101 catgatccgc cgaacccgcc cagcaccgtc caactcgggc cggaggaata tgatgctcca
     5161 tacaacgtgg cctaccacgt gttccgaatg accggggtcg agattgtggg cgatggcggt
     5221 gcgaaggaaa gggtggtcaa gatctacgtg gacgagcgtc cgaacccggt gatcacggtc
     5281 agcggcacca agagcgccgg cgccgacggg ttggggtttg gtgccaccaa cgccttgagt
     5341 tggcaacacg tctggtttga ttggatcaca ggaacgaacg ccggcgcgtt tgcccctggc
     5401 gaggaggtct catgcatcgg ccgcagcctg tacttgggcg ggcgctacga cccctgcaat
     5461 gaagtattcg ccgatgcgga cggcgacggg gacgtagatc aggccgactt cgccgtcttc
     5521 cagctctgct acacgggtat ggacggcggg gtgttgaccg atcccgatta ctgtcagtgc
     5581 tttgatcgcc ggaacaacga caactcgccc ggccaagaca acgacgtgga cagtttcgat
     5641 ctggcggctt tcgaggactg cgccagcggt cccggagtgc cggccgatcc ggagtgtgat
     5701 gactgaacgg gtgggcagag aaaggcgtga ggagagcaag catagcaagg tccggttggc
     5761 ggcttcgggc ggactgcggg tcgcggggtc cgcgccggta ctgaaaggag gcgtgaagag
     5821 cgatcaaaaa gatgacttgt gcagtagttg acgatagccg cgagcacgtc gtccggccgt
     5881 gatcgagcgg cattgtgccc tccgcgagaa caggcgggag cgttggaaaa gccggagagc
     5941 gaaaggcgat tgttagatag gaggtaggaa catgcgaaac atcatacgca tcttggccgt
     6001 cttgggcatg gtcggcacgg ccggtgcggc tgagcactgg cgaggtgcag gctggggcgg
     6061 agatggcacc agttggcacg acgtgctcaa ctgggatgac tcgattccgt cggccacgac
     6121 gtgggatgtc aataagacgg gtgaacaggg cacccccggc aatcacaata tccgcattac
     6181 ggcaaatggc acctgcggca gcgggacgat ctataaccag cccggctata ccgtcaacat
     6241 gaccgtggaa aacggcgtca cgatgaccaa cagcggggcg ctcaactatt acgcagcggg
     6301 tctcttcaca gtcaagaccg gcgccttcta cgacatgaga aatggtcagc ttgccatcga
     6361 gcacagcaac ctcaccacgg tcattgaaga caacgcgcat ctttactgca gcaccttgcg
     6421 gcacaacaac ggcacgaagg tggatgtgta tggtttgctg gaggcctgga gcgtaggccg
     6481 gatatccgcc gaccccggtt acagtctcaa cgtgtacgac ggcggggagt tcgtggttta
     6541 cagcggcgtc cccgtcggcc catggagcaa ggacggagtg gttaccatgt tcattggttc
     6601 aacggtcacg ctcaacggcg atcactcggc gccgttggat tacctggcca aggttcgggc
     6661 gggtgagccg ggtacgtggc aagttgagta ttccaatggc tggaccacga tcatgctcgt
     6721 tcccgagccg gcatcgctgc tgctgggtct gtgcggtctg gcgatgttca ggagacgcgg
     6781 ggcctgaggg aggggagtag gcgttcaggc gtgtttaatg tcagtgaggc gaaaagcatc
     6841 gagtatgctg cgtggtcgtg gtgatggcga ctgctcttgt gcgggcatgt tcagccaagc
     6901 cacgccagca gaaaggagga taaagcaatg gtgaagagaa gtgttgttgt tgggatggtt
     6961 gttctggcct gtgccatgtt ttgtggctgg gcgtcggcgg cggtgatcgt ctggaacatg
     7021 ggcgaacaac tcaacgacgc cggcaagcag acttgggcca tgacaaaggt tggagacggg
     7081 tatggggata ttggcgccgg cgatttcaac cccccgacca acgcgggggt caacccgatc
     7141 gtgcaaggtc cgtacaccgc gttctcgcga tttgtggact ccggcagcta tgggaatgac
     7201 gggttcgtca tgaagaacac cgtcggcacg cccatcggtc agactttcac gatcgacatg
     7261 atcgtcaagg gcgagcggaa gctcaactcc accggccgat ccatggggtt gagagtcggc
     7321 aactccggcg ccaccattag cgccggcgtc ggaatcgagc cgggcacagt ctttaacccg
     7381 gcatttgacg gcaacgcgtt atggatgacg tgcaggaacg gcgcttacat tccgcacggc
     7441 gcccttttcc tcaagacgac ggacgctttt gagtggcgcg ccgttcggat taccgcaaac
     7501 ggcaacggca ccgtcacgct ctatgatttc ctgtatggag cggcgggcta cgtgggcact
     7561 gtgccagaag gcggctattg gactaatctg ccagcgggtg ctggcggcga gttggacgcg
     7621 ctcggcttcc acctcaacag cctgtcgggc gggaccgtga cttggagcgg tttctcgctg
     7681 ctgggcttgg cgatgaatac tgacacggcg ctggacggga cgactccgtt catgctcgcg
     7741 gccattccgg aaccggccag cttgttgctg tttgccttgt ttggcgggtt gaccctgatt
     7801 cgaagacgtc gctgacgttg tcggatcgtg aacacaaggg ctgttttctc aaacagcccg
     7861 agtaacaaga acaggaagca ccggtcacag ccggtgagcc atgggcggcg gcggacgttg
     7921 ttgccgggta aggcaagcga cgggagggcc gttgttggcg agttggtttc agcgggttgg
     7981 ccgctgaaca agcccctgtg gcgatacccg gatggcaggg gcggcagtgg tatttggtat
     8041 tacctgactc cgcaacgggg gcacagtgcc ggatgcgtcc gaagacgcag ccgggggcag
     8101 cgggtgttgt gggaacgaaa gcgctcccgc aacgcccgca tttaatctct atccgaggcc
     8161 cagtggggat cgacctgccg ccgggtccgg cgattcgggc tggtttgcgg cgccgtcgtg
     8221 gggtgcaagg ttggcgcccg ccttctcaag ctgcgcgggg caaacaggcc ttcagcgtga
     8281 cgggcgggat cagggtcttg caccggcagg agttgactgt ggcaaatgtg ccggtcgaac
     8341 ctgcgtggga cggggcattg ttgggtgtag cgggcgagga ggagccatga cggttgatcg
     8401 atatgcgcgg accctgccgg tctgtatcac attgatcctc accctgccgg caggcgcggc
     8461 cacactgggc atttccgccg acggccggtt tttcactatt gacgcggtgc cgacctatct
     8521 gctgggggtc agttactatg gtgctcagac catcaacacg cccggcttcg tgacccagga
     8581 tctcgacgac atggtggccc ggggattcaa ctggatccgg gtctggtcca cctgggatgc
     8641 ttccgacaca ggtagcggcg attgctccgt gctgagcccg gcgggcatag tgcgcgaacc
     8701 ctacatgacc cgcctgaaaa cgctcattgc cgagtgcaac agccgcggga tcatcgtcga
     8761 tgtcaccatg tgccgcggtt ccggcgggcc gaacaacctg gccgagcact tggcttacgc
     8821 tcaagccctt gcgactcacc tcgcctcgta cacgaacatc tacttcgaca tcgccaacga
     8881 gtgcgacgtg ggtgattcga ggtacgtgcc gccgtcggag atcacacaga tcgtcgcccg
     8941 ctgcaaacag tacaagcccg gcgtgctctg cacggcctca cagggggcca ccagtgcctc
     9001 cgatctgtac aacaagtaca tcgccaccgg gtgcgatttc atggctccgc acctgtgccg
     9061 cgaccagggc tgcgccgctc aaacgtatgg aaccgtcacc gacttcattg cttggatgag
     9121 ccaatatggc cgccgaatgc ccgttcatct gcaggaaccc ttccgtcgcg gctatggaag
     9181 ctacaatccc gtcctggacg atttccttac agattgtgcc ggcggcaaga atgcgcaagc
     9241 agcaggctgg tgtttccata acggctggaa taacggcaac cgaccctatc ggtgcttcga
     9301 catgaaagac agcgagggac ggttgtttgc ccagattgat tctgaggaat ggcgggtggt
     9361 taaccaggta tacggacaca tcagcggcac ggcgctggca tcgggggcgc cggtgattcg
     9421 ggaagtcgct cctgatccag ccactacaaa cgttggcgtc cagtacttcc ggcagatggt
     9481 tctgactcag ggcaatccct ggccggcctg gtcgatcctg caagggccga gcggagccca
     9541 gatcaacagc aaggggcagg tgtcttggac cccgacttcc gccgacatcg gacagacccg
     9601 caccttcgag gttcaagcca ccaacaccaa tggctccgac accgagacct ggcaggtgat
     9661 cgtgcaaacc ggaccggtcg ccacgttcga ggccgagggg gcaggcatgg ggcaccagat
     9721 cggccgtgcg attaccggcg gctggcgagc cgtcgtgacc gacacagtca acctgttcag
     9781 cacctacggg ccatatacga caagcctgcc gacgggcccg ctggtcgcga ggttccggct
     9841 gcgcgtggac aacaacaccg ccgacaacgc gggtgtgtgc cagctcgacg tgcacgactt
     9901 tgacggaggc acgctcctgg ccggtccggt caccatcacc cggcagcaat ggactcaggc
     9961 caacgtctac cagtccttcg acctgagttt cgtcaatccc ggcaacaacc atcggctgga
    10021 gttccgcacg tactacctcg gagctgccca gttggacgag gataagatcg acatctggta
    10081 tagcgacggc aattatcctc cgtcggtcag cgccggggcc gaccagcagg tctcctggcc
    10141 cagtgtcgtc aatcttgacg gaacggtcac cgacgacggc ttgcccaatc cacccggcgc
    10201 gaccacagtc acatggagca aggtcagcgg gccggggacc gtcaccttcg gcaacgccgg
    10261 cgcggtggat acgacggcca cattcagcat ttcgggtgtc tatgtgttgc ggctcagcgc
    10321 cacggactcg atgtataccg ccaccgacga cgtcaccatc tatgcgtccg gcgccggcga
    10381 tttcgtcatc tcggcggagg cattcatcgg ttccggaact ctgggcgata cgcaaaacgc
    10441 ctggcagctc gtgcacggac ggtgtgactc ggccggcaat ctccacgtgg tctggtacaa
    10501 gaacggagcg attgcgtatc gggtgagact cgccggcggg acctggggcg ccgaggagac
    10561 ggttcccgat tcgtccggca tcaacctgga cgggccgcgc ctggcggtca cgcccggagg
    10621 ggacgtgcac gtcttctgga acccgccggc gtggaacccg ctgtattatt ccattcgcac
    10681 cagcggcggc tggggggccc gccagaccct gatctacaac gctaacggtg atcacgcttc
    10741 cactcccgtg ccggtgttcc tcgccgacgg gcggctgttc ctgatatggc tgcgttggga
    10801 tgggacggac aactggtact accgaatcaa gacaggcgga acgtggagct cggatcagcc
    10861 tgtgagtccg catatgtatg acggctacgc cgtggtcacc gacggctcga acaacgtcta
    10921 tgcggccggc cgggttctcg gcaatccgca ccgggcaggg taccaggtgt ggaacggcac
    10981 ttggggagcg gtgcagacgc ccgccacaga cgttaatcaa tgggtgggcg gaacgcacat
    11041 cgcttaccgc tccggctcgc ctttggctct gaccttcgcc ggttacaacg agagcggagg
    11101 cgtctggtat atcgaaggcg tttacagtgt tctcgtcggc cagccttccg ttcggctgac
    11161 gcaggcaacc cggtgcggga cgtccgtgcg ttgtgaggac atcgatccca aggtcgtgta
    11221 cgattcgcaa ggcaatcatt actacgtgtg gaccgacggg gaccagctcc gctacgcgat
    11281 tcgcaaggcc gatggaactt ggtacgccaa ggaccagaac ttccccgtcg gcggcggcaa
    11341 gcaatacttc cccgatatca cggccttcgg tgacacggtg cacgttctgt gggaggacag
    11401 ccgtggcggc atgtaccatg ccacaatcat gccccccggc ggcaactctg ctccgcaggt
    11461 gaatgccggt ccggaccaga cgatcacctt gccggccggc gcaaccttgg atggaacggt
    11521 aaccgatgac ggtttgccga acccgccggg acaagtgacc gtgacctgga gcaagcagag
    11581 cgggcctggg gaggtcatct tcggcgacgc tcacgccgtg gacacgaccg cgtcgttcag
    11641 ccaggcgggc agctacgtgc tgaggctctc tgccaccgac tcacaactga ccggtgagga
    11701 tgatgtgacc attgtggtga atgccccgga tatccccgac tttgacggcg acaatgacgt
    11761 ggaccagtcc gacttcggtg tgctgcagca gtgctgcagc ggttcggcca aaccctacgc
    11821 gcccgggtgc gaaggcgccg acctcgatga cgacggggat gttgaccaga gcgatttcga
    11881 cgcgtttctg acctgcctgg gagggcctgg gcagccggct cagtgcggac cttgagccgt
    11941 tcgcgggccg cgtgaggcgg aggtgaacag cgggacggga tgtgatcgcc aacgcgacag
    12001 cgggacaatg aaatgcctga agaaccatcc tgttgattgc agcaggcggc ttgccccgag
    12061 gcgcggctgt gcgcccggct cagcatgggg gcgcgtgacg agcgtgttca cggcggcgat
    12121 tcttggggcc atgccggggg gcatcggcgc ctggggacag gcatgtccca aggaggcctc
    12181 tccgtacggc atcaatgcgc acgcgccgga aggccagtct gtttcccggc tgctcgatga
    12241 agtctgcgcg tgcgggattg actggattcg ggtcgatttc gtatgggcat ggattgagac
    12301 ggcccaagac agcttcaact gggcaaggta tgacacaatc acctcagcgg ccagggcgag
    12361 gggtctgacg atcttcgcaa ccatcgggca cacgccggca tgggccaccg acggcagtga
    12421 aggcagcgga gtgcctcgca gcgccgcaga ctactatgac gtgtgctatc gggctgcgct
    12481 gcgctaccag gatagcgtcc gtcattgggg aatgtggaac gagccgaacc ttgacggctt
    12541 ctgggcgggc acgcgccaac agtacatcga catgatcctg aagaatggtg cggacgcggt
    12601 gcacgcggcc aatccggcag ccaaggcgtg cgggcccgag ctggcccacc tcacatcggg
    12661 tgaccaggac tggtatgcgt ggctcgctga ctgcatcacc caggcgggca gcaagctcga
    12721 catcgtcacc caccacgttt actgcagcag cggatatgcc agttgcacgg acaaactcga
    12781 aaaggccccg ttctggccgt gggacccgcc gtcagtcaaa caggtgctgc agaatgccgg
    12841 atggttcggc aagccgttct ggcttaccga gatcggttgg cagagtggca gcgtggggga
    12901 ggcccaacag gcgacgaact atactgggtt cctcaccgac tggttcacgg gcaagtcggg
    12961 gcgtggatgg gttcacaagg tattcttcta tgaactcaac gacacccagg cgtttccgga
    13021 cctgtcatgg ggtattctgg ggcctgaccc gacctacgcg cgaaagccat ccttcaccgc
    13081 ctatcagagc tttatcaccg cccatccggc gcccgcaccc ctgccggggc aggcatatga
    13141 tccagaacct gctgatcttg cggcgggtgt gagtttgacg ccgcagctcc gctggacggc
    13201 gggcgactgc gccgagtctc acagtattca tttcggtctg acgaacccgc cgccgttcgt
    13261 tggcaatcgg acaacgacta acttcgctcc cggtacactg agtcctcaga ctatctacta
    13321 ttggcgcgtt gatgaggtca actccgccgg aaccaccacc gggacggtat ggcgatttca
    13381 gaccggcgcc gctccagggg atttcgactc ggacggcgat gtcgatcagg aggatttcgg
    13441 gcatttccag gcgtgtttca gcggcccggc gcagccgttc acgcctggtt gtggcaacgc
    13501 ggatatcgat gatgacggcg acgtggacca gactgatttc ggcttgttcg aatcctgcat
    13561 ggcaggtccg gacctgccgc cgacgtgcag ctaggttcgc gaccgacagt atcggggacg
    13621 caacatgatc acctggcgaa ccaccatgtc ggtaattgcc ctggcgggct tgcttgccac
    13681 cggagtggtg ccggcagaac cgcctgacgg caccgtgttg cactccgcgc cgtttgatga
    13741 ggaagaattc tccaagtgtg gatcgctgag tccgcgagga cagatcgtat tgcgccagaa
    13801 cacctatgat ttcgctgatt actatctgta cgtgccggct ggagccgtgc cggaagtgcc
    13861 tttgccattg gtgatcacca gtcacagcac agtgaccacg gccgacgagg aaatcgggct
    13921 ccagccggac cccacatggg gctgctgtga ggaatcgccc tatccgcaca cgaggtggta
    13981 tgaactggcg gaaaacgccg acaacgtgaa ccgccacaac cagtggtttg ttgtggcggc
    14041 gccggccatg tccagcgcca acggcgggct ggtcgaacct tggaccggcg agttacaggc
    14101 aaccaccgat gagcggcggc ttctggccat ctacgatcag atcgttcaca cggaggcagc
    14161 ggcatatggc ttcgcccttg acgcgagccg cgtcctgctg accggatggt cggggggcgg
    14221 cattcccacg tactacgccg gcgtgcgtca ccccgagatc ttcagcatga tcgtctcgcg
    14281 gcagggcaac gtcgacgaag acatgtttga cacggccgcc gtgccgctca acaccaatct
    14341 gctggtcgcc attctcaatg gcctgaccga cacggtggtg ccgcgatcaa agcatgaagc
    14401 cgcgcaagat tggctgatcg gcaacggtta cccgcgagtc ttcgtttcgc ccgccgatcc
    14461 gcacattcgc gatctgccgg atgccgaatt tcccgatgtg ccctcaaatc actattgcca
    14521 ctcgctcgtc acgttcgaca cgttcatgga tgcgatgcct cctgtgggac ccgtgatcgc
    14581 tgccgtgacg ccatcgccgg agatcgtgac gcctgggtcg cagtaccaaa gacaactgag
    14641 gctcatgcag gggaccccac ctgtcgagtg gtctgttctt cagggtccga ccggcattca
    14701 ggtgaacgcc gccggcctgg tgagcggctg ggcatcgggt gttgccgatc tcggcgaaac
    14761 cttttcgttc gaagtgcagg cggacaactc catggggtcc gacgtggtca cctggcaggt
    14821 tcgcgtgttg tcagcgtgcg atttcgataa ggatggcgac gtggatcagg cggatttcgg
    14881 ccatctgcag ggctgcctgt cgggacatgg gcagacttgt tcgccggagt gcatcgacgc
    14941 cgatctctcc tccgacggag acgtcgatga ggttgatttc ggcctgttcc aagcctgttt
    15001 ggccggaccg gattgcacac ccggctgttg atgggaggac gctaagtgag acagctcttg
    15061 ttgccatcgt tcatcgtctg ccttgcggcg gggacagttg cattagccga tcctgtcaat
    15121 cctgccgcct caactcccgc cggcgaaacg ctcccgccgg gatacgtccg aggcgacgtc
    15181 gtgagctatt catacgttcc caatcggtac tggacccggg cggaagcctt cggcatctgt
    15241 ccttacttgt tcgagtacgg gctgataaag tgggaaagcg acgcacccct cgagacgttc
    15301 catgccgaga acatcaggcg tatcgacatg gtcagcaagt ggtcgacggt catcgggcta
    15361 tgcaaccgcc acagagaatg gcaggacgaa gtcgtcaact tgatgatccg ctgctttcgg
    15421 aaccgccaac tgattctgct cccggcccgc ccgagcccta ataagccgga gaaggactct
    15481 ttcaagcatc ttattgcact cttggagagg ctatggaagg accgcgacaa gaccctggtc
    15541 agtcccgagg gcgaccgtgc gaccggccgg cagttgatca acaacattct catggccgat
    15601 ctgggcgatg agggtctcag cattctcaag acggagggat tgaccaagct gaaccagagg
    15661 tttgacagag agattcgtaa tcggctgatc gatggccagc gcccgttccg tcacatcaag
    15721 acctggtaca acgaggttca ttgcggcatt ggctgctatg cggccaacca ggaagatctg
    15781 gccaagggca aggtgaaatg gccggcgaac tctgagttca tcggtgttga cgtttatcac
    15841 tactggagtt ttgaccatac cccttttgac ccggacgatc ccggggtacc gcgcgaccga
    15901 gtgcttcagc acgccattgc ctggcagaac gtcatcacga aatactacgg ccccgatttc
    15961 cgcgtaaccg cgggtgatgc ctgggaccac aagcacaaga acgacaccca cgccatgctt
    16021 caggcgatcg acctggccgg ggccagccgg gcgatgatga tctatatcgg caacacgcag
    16081 tatgttgtcg gctcatgcta caccacgccg atcgagacca tggacgcgtt ctacgactcg
    16141 gtgaaggccg gcccctgggt cggcctgagc tggtgggtgt ttgacaccgg cggcgtcggc
    16201 gggggaacgc tggattacct ggacggcacg ctccggcact acacgccggc caacaagaag
    16261 gggaagccct acacgacggc cgagctggaa aactaccgca agcgtttcat cgcttcgcgc
    16321 atgcggatgt tcaacgacgt ggtgtacaac caattcggat acctcaacgg cccggcaccg
    16381 aagcagtaga ggacgataag gacgcacgca acatgcatag agactacggt ccgatgtggg
    16441 tgaaggctgt tgctctggtc gccgtgatgg ccgccggtcc tgccggcttc gtgttgggag
    16501 cgggggcgga tgtcagaaca tcctcgcggc cggcggggga gcgagccgag gggttcccgc
    16561 caggccacgt tcgtggcgat gtggtcagct actcgtacgt ccccatccgg acctggagcc
    16621 ccagcgaaag cataggaatc ctgccctacc tgttcgagta cgttctgacg acgctcgacg
    16681 accgcaagcc ccttgaagcg tttcacgcag agaacatccg ccggatcgac attgccagcc
    16741 aatggtccac cgccatcgag ctctacaacc gacgtgtctc gtatcgtgac gagatcatca
    16801 atctgatgat ccgatgccgc agaaaccgcc agttgatcat cctgtggggc cgcccgtcag
    16861 ccagaaagaa cgacgacaag gacacttcca ggagtctcac tgagatcctc gagaaactct
    16921 gggccgacag ggacagggaa ctggtcagcc cggaagggga ccgcgccacc ggccggcagc
    16981 tcatcaacaa catcctggcc gtcagcctcg gagatgaggg cgaatgcggc ctgggaactc
    17041 aagggctcga gaagctgttc gcgggttttg accggaacgt gcggctgcgt gagatggatg
    17101 gccaacgtcc cttcagccat attaaaggct ggtacaacat gctcagctac acggcgctca
    17161 actacaacgg ttgttatgcg gccagccaga cagacgtgga cgagcacaaa cgcgtcaagc
    17221 tgccgctgaa tacccaggcc atcggcgtgg atgcttacca ctactggggc cacaagcaca
    17281 gcccgttcga tcccgcggat ctgtccatcc cccgggcgaa ggtgcgtgcc cattcggagg
    17341 aatggcagcg gattcgcacc cgatactacc ctgaaggtgt ccaagtgcgg gtctgcgaga
    17401 acagcggcga tccccagaca tggacgcccg aatgctggag cgatacacac gcactgatga
    17461 gcgccatcga gctggccggc gcgaagaacg tcatgatgtg gtacatcgcg gcgtgcggcc
    17521 agatcgaggg ccagagctac accacgccca tcgagacgat ggaatcgtac tacgagcatc
    17581 tcaaggccgg accgtgggtg ggcctgtcct ggtggacctt cggtggtttc caggagggag
    17641 gcgaacacgg ggctcatttc atcggaggca tcgagtacta cgatcgggtc ctcaagcatt
    17701 acacaccgca gcatcccgag ggtgagccct attcggccga gatgctggac tactggcatc
    17761 gggagtacgt ggcgctcaag atgcgcatgt tcaacgatgt ggtctacaac cagttcggcc
    17821 acctcaacgg ccccagacca atagcccaga gcaaggagta gatcgatgtg cgggaagaac
    17881 ggttgtctct tctctgctat ggtcatggta tttgccgcgg tcagtgcaca gacccagggc
    17941 ggcgtctggc gaggttcgac gacgcggccg tcgggggaga agctgcctcc cggctacgtc
    18001 agcggggatg tcgtcagcta ctcctacgtg cccatccgca cttggacccc caaggagggc
    18061 atcgggatcc tgccatacct cttcgagtac gacgtgatgc tctggagcgg aaagcagcga
    18121 ctggaggact ttcacgccga aaacgttcgc cggatcgaca ttgccagccg gtggtcgacc
    18181 gccatgccgc tctacaaccg gtacgactac tatcgcgacg agatcatcaa tctgatgatc
    18241 cgctgtttcc gcaatcgtca actgatcatc ctcagcgcca ccccgaacac cagggacaag
    18301 gaaaaggacg ctcataccaa cctgatcgcg attctgacga ccttgtggca gaaccgagac
    18361 agggaactga cgaatccgga gggagacaag gcaaccggcc ggcagcttat caacaacatc
    18421 ctggccgcca gccttggtga tgaaggcgaa tgcgggctga agacagccgg gctggagaag
    18481 ctgttcgcgg atttcgatca cgagatccgc ttccgcgaaa tggatggaga acggccgttt
    18541 cgtcatatca agggctggta caacatgctc ggctatgcgg cactgggcta tgacggctgc
    18601 tatgccgcca gccaggagga cgtggacaag cacaatcgcg tcaagctgcc gccgaacacc
    18661 caggccatcg gcgtggacgt ttatcactac tggttccaca agtacagccc cttcgacccg
    18721 gccgacctgt cgattccgcg agagaaggtt cgcgcccaca gcgacgagtg gcagcggatt
    18781 cgcacgaggt actatcccga ggggcttgag gttcgtgttt gcgagaatgc caaggatccg
    18841 gccacctgga ttcccgaatg ctggaacgac acccacgcgt tgctgagcgc gatcgaactg
    18901 gccggggcca agaacgccat gatgtggtac atcggggtca gcggccagct cggcccggcc
    18961 ggcgatgaga ccacctacac caccccggtt gagacgatgc aggcctacta cgacgagttg
    19021 aaggccggcc cgtgggtggc cttatcctgg tgggtgttcg gcggctccaa cacctgccat
    19081 gggggattag agtattacga caggaccctc aagcatttca cgccccggca tcccgaaggt
    19141 gagccctatt cgcaagagat gctcgactac tggcacgacg cctatgtgaa gacaaagatg
    19201 cgcatgttcc atgacgttgt ctacaaccag ttcggccacc tcaacggccc catgcctgtg
    19261 acccaggaca agaaggagta gctgaatgtc tcgaaacgaa cggcaccgat gtttgattgc
    19321 ggtcttagct gctgcggcgg caagtacgcc gagccgcggc gacgtctggc gaggttcgac
    19381 gacccggcct tcgggggaaa agctgccccc tggctacgtc agcggcgacg tcgtcagcca
    19441 ttcttacgtg ccgattcgca cctggacgcc caaggagggc atcgggatcc tgccctatct
    19501 cttcgagtac aacctgatgt gctggagcgg cgaccaacgg cttgaaaagt accacacgga
    19561 gaacgtccgc cggatcgaca ttgccagcaa gtggtcgacg gccatccaac tctacaatcg
    19621 ctacgactat taccacgacg agatcatcaa tctcatgatc cgctgttttc gaaaccggca
    19681 actcatcatc ctcagcgctc gtcggggtga caggagcaag gacagggaaa aggatgctca
    19741 cgcgaacctg atcgcgattc tgacgacctt gtggcagaat cgagacaggg aactgacgaa
    19801 tctggaggga gacaaggcaa ccggccggca gcttatcaac aacatcctgg ccgccagcct
    19861 tggtgatgaa ggcgaatgcg ggctgaagac agccgggctg gagaagctgt tcgcggattt
    19921 cgatcacgag atccgcttcc gcgaaatgga tggagaacgg ccgtttcgtc atatcaaggg
    19981 ctggtacaac atgctcggct atgcggcact gggctatgac ggctgctatg ccgccagcca
    20041 ggaggacgtg gacaagcaca atcgcgtcaa gctgccgccg aacacccagg ccatcggcgt
    20101 ggacgtttat cactactggt tccacaagta cagccccttc gacccggccg acctgtcgat
    20161 tccgcgagag aaggttcgcg cccacagcga cgagtggcag cggattcgca cgaggtacta
    20221 tcccgagggg cttgaggttc gtgtttgcga gaatgccaag gatccggtca cctggatccc
    20281 cgaatgctgg aacgacacgc atgccctaat gagcggcatc gaattggctg gggcaccaaa
    20341 cgccatgatg tggtacatcg gcgtgtcaag ccagatcgac aattccagcg gcgcggcgac
    20401 ctatacgaca cctgtcgaga ccatgcaggc ctactatgat gaactgaagg ccggcccgtg
    20461 ggtggccttg gcctggtgga cattcggcac cgatcgggac acgcacggtg gcctcgagta
    20521 ctacgacaaa acgctcaagc atcatacccc ccgacatccc gagggcgagc cctattcaca
    20581 ggagatgctt gactactggc accgggcata tgtagacgtg aagaagcgga tgttcgaaga
    20641 cgtggtgtac gggcagtttg gccatttgaa cgggcccgtg gccaagccgt gaggggggcg
    20701 gcatcactga ggctgaacca tcgtccttcg gagtcgcaac atgcgggata ctcgcgtggc
    20761 agcagctcag tttgaacatc gcgacggaga ccaggcgtac aatcccgggc ggatttgcga
    20821 tccgactcgc aaggcagtcg atgacggtgc cgagatcgtc tgctttcacg agctctggat
    20881 gccggcctct gggtggattc agccgacgcg cccagaggag gccggccgcg atcaccgagc
    20941 ccgcgccgaa tgggccaagc atccgggagt cgacccgaat cgccagggat cattcgacca
    21001 ccatgatggc ggagcttctc gagatcgacg gcgaacgctt ctgtaactgc tacgtaaccg
    21061 tggggcaagg tggccacata accaagcagg ctgcatccag cgacgctgaa gcgtattcga
    21121 aggcttctgt cgttgttgaa gctgctccac ggaggcgaac ttcgtgaaac gatagaaagg
    21181 cgaggctcca tgacaatcta caaatccatc atcccgtttc cgcgcttacg aacggcttcg
    21241 gggccgggtt tgcgcgtggt catgattggt ctggcagtga gtgtgacgat cgtccagggg
    21301 gctgaaacgc agcaaatttc ggccggcgtt cggacctcga ggccggatcc ttatgccccg
    21361 acgacgcttc ccgatcgcat cgtacttacc tggtgtggcg acccgaccac gaccatggcc
    21421 gttacctggc gcacctcgcc ggatgttcgc aaggggtttg cggaaatcgc tccagccgag
    21481 caaggccagc ggttctacca ggagctcaat gggaatgaat acaagaagat tacctttgac
    21541 aaccagcagc ggacggtcga agcacagacc acgagcttcg agtcccccgg tccgcgctcc
    21601 tgttaccatt cggcccgttt cgaaggactt tctcccggta ccaggtatgt ctaccgcgtg
    21661 ggcgatgggg tcagatggtc ggaatggttt cagtttcgga cggccacgcg cgaggcagag
    21721 cctttccgct tcatctactt cggcgacttg caggatgccg tcgcctcgat gtgttcctgg
    21781 gtgttcaggg cggcgattct atcggctccc gatgccgcgt tcacgctgca tgcgggcgac
    21841 ctgactgcca gccgtggcag cgatgtggaa tggggcgagt ggtttgcagt gggaggctgg
    21901 gtcaacgcaa tggtgccagt ggtggctacc ccgggtaacc acgactactt caagacggct
    21961 cccgacggaa gcccgggcgc ctacctggaa ccgcactggc gggctcagtt cacgcttcct
    22021 gagaacggcc ccgctggtct cgaagagtcc tgctactata ttgactacca gggagccagg
    22081 atcatatcgc tcgactcgaa cgtgcggcgt gaggagcagg cccaatggct cgaccgaact
    22141 ctgtcgagca accccatgcg ctgggtgatc gtgacttgtc atcatcctgc atactcggtc
    22201 gccaaccatc gaggcggagg ggtgcgccgg gaatggcaat cggtgtttga caagcatgac
    22261 gtggacctcg tgctcacagg gcatgatcat gcttacagcc gcaccggtct catcagaaga
    22321 gggaaagacg atacgcctgt ggaagggcgg ggagagggca cggtttacgt cgtgtccgtc
    22381 gccggtccga agatgaactg gccgattgtg gcaaagggcg agccgacagg cttcgccaaa
    22441 cgcgaggcgc aggacacaca attctttcag gttatttcga ttgacggaga caagctgcga
    22501 tacgaggcct ggacggccac aggcgtgctc tacgacgcat tcgcgcttgt caaacaaggc
    22561 cgaggcagga aggagctgat cgaggagatt cctgccgcgc ctgctcgccg ccgaggccag
    22621 ttgggctcgg cgtcggggcc cgatcgcggg gcggtcctgc tgccgccgga gaagtaggtc
    22681 ggtgggcgcg cgaggggtgg acgaacgtaa gtagtcgggc ggcggggcgt tccaacggtc
    22741 ctctgcccgg caggtgtgca gtcacatgaa tctgtcggag acgcgtgatg aagaaacggt
    22801 tgacactggt tgtgggctct ctcttgatgc tggtcgtacc ggccgcggct gagccggctc
    22861 cgctggccgc ggtcaactcc gcgggcgtag aacgcgatcc ctggctttcc ccggacggct
    22921 acacgctgta tttcgcatcc aaccgttcgg ggggcggttt tgacatctac cagtcgcagt
    22981 gggacggtca ccagtggtcg cagccaaagg cgcttggcag ccacgtcaac tccgattttg
    23041 acgacattcg gccgagcttg tccgccgacg gccggcgact gttgttcgtg tcgcatcgcg
    23101 gccgcttcta tcgaggcgtg tggatttgtg atcgtcagcc ggacggatcg tggggacaag
    23161 cgcgatacgt cacggtcgac gagcaacccg ccgtcgacga cgcggtgatc gggcgggatg
    23221 gtcggaccat tttctacaag cggatcagca agaatcagcc gccgacagac cggcggtggg
    23281 acatgtgcat gaagtacgat cctcgccggc gtttctggga ggtcccgcgc ccctatcacg
    23341 gcccgttgcc agagaaccat gttccgggag cattttggcg attctcggtc aaagacggcg
    23401 acatttatgt cgaagccgcc accgaagcgg aaccccaaaa gacggacgta ccggaggtgc
    23461 tgaattgcct gaagacttcc agtctttatg ggagggaaga ccaggagtac tgggtgtccg
    23521 aggggcagaa cctggtggac gggcttgaag acacaagctg gatttcgaag gaggggttgc
    23581 ccatcaacca gcagtgggtg ttgctgggcc tgaacggccg agcgaaatac gttcccggtc
    23641 tcagtcggat tcatcgtttc cgtattcact ccggcccggc cgaggagcat cgagatcaag
    23701 agcgcaagga cgcggttgtg attttcgaca aggaaaaggg gcccccggcg cgcccgaccc
    23761 gattgcgtat tctgggcggc atggatgtgg actcgttgaa ggaattggcg gtcgtcgagt
    23821 tggaccttaa cagcggttcc ccttggcacg acgtgccttt gaaggagccc acataccttc
    23881 gctacatcaa gattgaagtt cttgagacgg cggggcctgc cgcggattgc gtcgctttca
    23941 acgaggtcga ggcgtacggc gccggcctca gcgctcgacg gcccgcgcat cgcgtttcaa
    24001 cggatgagaa caacaacatt ctggtcgacg gcgagccgtt tttcccggtt tacctttact
    24061 atgccaaggc cgaggcatgt ttggccgatt acggcttcaa taccacgctg gaaacgtacg
    24121 acgtcgcccc cgacagcgcg cggctggcca ttctcgaccg cgctgaggat tcaggcttca
    24181 tggtcatagg gcatgtgccg aacgtagatc acgaatttga acggaagagg gctcgcaacc
    24241 agctcctggc cttgaggcat cacccggccc ttctgggcta cctcatgtcc gacgaggccg
    24301 gtcacagccc cgagatcatg gccatggaca agcggcgatc ggacttcatc catgaacacg
    24361 acccgaatca tttcacgatg ctgaatgatc tatatccgac ctactatccg cgttcatcgg
    24421 cctttgtgga cgtcttttcg atcgatccct atccccacat cgtcggacag ccgttctcat
    24481 accagggtta cgcggttgac gtagcgtatc agtgcgtcca gcacaagaag ccggtgttcg
    24541 tggtcaatgc gtcctgggga ccgattctat ctccggtgga gaaccggctc ggtgtctacc
    24601 tggctctgat tcacggagcc aagggcatct catggtacgc gctgggtgtc aggaacgagg
    24661 atgacgacca ttacgcgagt atcctgcgct gcacgcggga aatccgtcga ctcgagccgg
    24721 tgtggtttgc gcctgacccc aagccggatt ccgctttgct gacccattct cgaatcgaga
    24781 atcccagtgc ccgaatcgac gtgatgatca aggaggttgc cggtgaggta tggttgttgg
    24841 ccgccaactg cgagcccagg caagccacgg tgaccttctc gttcgggtgg ggcgacgaga
    24901 ttgtaatcaa ggaagtcatg gccgagcaac cgcaggcctg gagcatgaat cgtcgggact
    24961 tccacaccat ggacgcctgg ccgccgtcgg aggatcgcga accggtgcgc caggctcgtc
    25021 ccattgatct gacgtttcct ccctatggcg tgcggatctt tcgcatgaag ccggtcggca
    25081 agatcgggcc gctatatcag ccggtgggcg acggtaccca actggccatg gtcaacgagt
    25141 cagcgatcag acgcacagcc gaacaggcca agtcccttcg ggagcaaggc aggctggagg
    25201 acgccaagaa ggccgtcgac gcattctggt cgaaatatgg aaagcgagtg tccgtggacg
    25261 acctttccag cctgatggac atactgggtc agaaagcatc agcagacgag atacttgctg
    25321 actatgggcg gctggcggag gagcaccctg aggccgaaag ctggcccaag tgggtctaca
    25381 agattgtggc agcactgcac gaagagaaac gcgatcagga agccggcgtt tggatgtcca
    25441 ggctgatccg ccaccggccg gactcgctct ggcgagccaa cgccgaagcg ctcgtcaatc
    25501 cagcgtctgc gcgagaaggg cacaagccct ggactcaggc cgtcaagatc gataccaagc
    25561 ctgttattga cggcgaactc accgagccgt tctggcagag ccgcgtgaag ttcaagaaca
    25621 ccgttttcct ggacagcagc aagaaccccc aggaaactga atttgccgtc gcatacgacg
    25681 atcaggctct ctactttgca gccaggtgcg tcgagcccca gccttctcag atcttgaagc
    25741 ggtgcgacaa agacgacggt tccgtctggg cggacgactg cattatcatt tatctggatc
    25801 cgaagctgga gtacaagtcc tattatcagc tcatttttaa cgtcctgggc acgaagtggg
    25861 acggcaccgg cagcagcggc ggccacgcga acgccgacgg cctgaacgcc aagaacgtgg
    25921 aacgcagggc agtggtcaag gacgatgctt ggcagattga aattgcggtc cccttcagtg
    25981 acctgaagcg gccggctccc agccctggtc aggtctgggg tttgggtgtc cagcgttggc
    26041 ggcaggtcgg cggggcattg cccacggttt ggggcaatga acgaggaaca tctcacgaca
    26101 gccgtccgga agactttggt tttctcgttt tccggtgaca tgaacgagca ggcagcaatg
    26161 ctgagccggg atcgttgcca gagcacctcc ggcgggctca cttgcgtcgg accgccttgg
    26221 tccacatggt ggtttttctt gttggtcact cctgcctctt ctgagggtgc cactggcggc
    26281 ttgtgagact tgtcaagagc ggcaacagca agcgcgcttg gtgaagcagt attccgccat
    26341 cgtctccgtc aacatcttcg cgagggcacc agcagcaccg ccctgttgcc cgtcgcggcc
    26401 ttggccggta gccttttgag ccgtggacat gcgagaagtg aaggaagtga agcttgcggg
    26461 tggggagtca tcagcgaaca cacaaagcgg cacatggcgc ggccggctat gtcgtttcct
    26521 ttgcagaccc ggatggccga tcctcgtcgc cctgatcgcg atgatactgg tgtcgccatc
    26581 gttgcgcctg gggtgggtgt ttgatgacga ctttcatcgt ctcaccattc ggggctcgac
    26641 gagatgcctc gcattctcgg gatcgatctg ggacatgttc cgctttctcg acggggatgc
    26701 tgcacgaacc ctggaattga tggaccacgg cgtgctgccg tggtggactg tgcccgaggc
    26761 caagggcagt ttctggcggc cgctgaccat tctgactcac tggatcgact accacctgtg
    26821 gcctgatacg gcgtggctga tgcacgcgca gagcataatg tggtatgggt tgctggttct
    26881 ggcagcggcg gcgctgtatc gacggatgat gggggcgggc atagcggccg ggctggccat
    26941 ggtgctgttt gcggtcgacg acgcgcacag catgcccgcc gcgtggttgt ccaaccgcaa
    27001 cgcgattgtc tcgggtgtgt tcggagtgct ggccattctg gctcacgatc gccggcggcg
    27061 ccaggggcga accggcagtg ccgtacctgc atggctctgc accgccgcca gcctgctgtc
    27121 ggccgaggcc ggcgtcggca catttgccta cctggtagct tataccgtat gcctggaccg
    27181 gtgcaggtgg cggcaaaaag tcttcatcat gcttccctac ctcacggtcc tgatcgtctg
    27241 gcgagttgcc tggacgcttg gcggccatgg cgtgcagtac ctcgggctct acgtggaccc
    27301 cttccgggag ccggcggtgt tcgccctgag cgtcattcag cggattcctc ttctgatgct
    27361 tggccagtgg gcgggactgc cggccgaggc tgcgattctt ctgccgaacc gtctcatgtt
    27421 gctgctccag cttctcggcg tggggattac cattgttctg gcggccgtag catggccgct
    27481 gcttcgccgc gatccggttg ctcgcttctg ggcgctgggc atggccttgt cactggtgcc
    27541 cgcttgtgca acgtttccgg ccaaccggct tctggtgttt gcgggcatcg gcgcaatggg
    27601 catgctcgcc cagtttcttt tagacgtcct gcggcttgag aaccactcgg gggccgtcgg
    27661 cggcttgtct gtcggtgctg tggctgacaa gcctctccgg gcacccggcc ggaacggtca
    27721 tccgcatgct cggcgtcttt gtcgtgtcgt cgcctggtcc tttgtatgcc tgcacatcgt
    27781 acttgccccc gtggccctgg ccgtgcaggc ggcatttccg atgggcttca agagattcaa
    27841 cgatgaactt acggtccgaa ccgcgctcga tggttccgtc gagcaacaga ctctcatcat
    27901 cgtgaacgcc ccgagcgtga tgcacgccat gtatctttcg gtgcagcggg aactggccgg
    27961 ccagccggtg ccccgccaca cgcgcgttct ggcccccgca ttgccggcag tcaaaatccg
    28021 ccggttggac aagaacacgt tgtccatccg gccggaagac agtttcatcg cctggcgctt
    28081 tgaccacctc ttccggagtg agcgaaggcc gatgtctctc ggacagcgcg tgaaactcag
    28141 cgacctgacc atcgacgtaa cggagctgac gcccgatctt cgcccggccg aggccctgtt
    28201 ccattttgcc gtaccgctcg agcacccgtc gctcaggtgg ctgcactggc aggacggcga
    28261 attcgttcct tttaccccgc cggcgatcgg cgaaaccatt gagcttcgtc cccggccgcc
    28321 cagtctctgg tgaggatcac gacggaatgc gttggtctgt tcctgcaggt ttttttctca
    28381 ctcattcctg atgacttctg cctactgctt gcatagtatc caatagaagg agatggtaca
    28441 ggatacgccg ctgttgctgt agccgggaga gtctccgccc atgcctaccg ccggtgaccc
    28501 gattgtcgat ccaacgaccg aacccggcag gttttcctgg ccgacggcat tccttgtggc
    28561 ggcggcgtcc ggcggactgc tggccgcctg cttccgtccc ctcggtctgc atttccttgc
    28621 atggattgcc ttggttccgt ggctgatgat gtttccgcga ttgtcgccgg ccgcgacgtg
    28681 gctttttggc atcgttctcg ggttggtctt ttatcggatc gggcttgatt ggctcatcaa
    28741 aatcgccggg ccgatagccg gcgcgaccat catcgttctt gcggtgtgga tgggcttcgc
    28801 gttccgggtc gcccgactgc tgacggtgcg attcggtcct gcgtccatgt tgtgggccgt
    28861 gccgctggcg tttgtcggag ccgaggtgct gagatgtgag gggctacctc gcctgcggtt
    28921 ttcctttctg gcattcgggt acagtcagtc gcacaatctc cgggtggcgc aaatcgcctc
    28981 gatcggcggc gtgtacttcc tgtctctgct tctggtggca gtcaatgccg cggtggctta
    29041 cggactgtgg cagcgccgtc gtcgggcctg gataccggcc gtggtgatgg cggcaggagt
    29101 tattgtggtc ggcgtcattt ctcagcctcc gtcgttcgtg cttcaggaca cagtgaccgt
    29161 cgcctgcgtg caaggcgaag agcttcgata tcgcgagtac ctcgatttag ccgcccgtgc
    29221 agcatcggct cggccgacgc ccgccttcat cgttttgccg gagcatacca tcacagaagc
    29281 ggcgacagaa gatcatcgaa tggtatcggg gctcgctgaa ctggcccgag aacacgacct
    29341 gtacgcctgc gtgggcgcgc acatcacatc gcaggccgga agctgtcgct acgataacgt
    29401 ggccatgctc atcggtcgtg ccggtatcgt cggcacccaa gccaaggcag ttcccttgcc
    29461 gttctttgac gacgggaatc ccgcccggtc acaagcggcg ttcgatacga cgtacgggcg
    29521 gatcggcatg tacgtctgct atgacggcct cttcaccgat caccctcgcc atctggcaag
    29581 gcttggagca acgctcatcc tcgttccggt gatggacccc gcgcgttggc cggcccagga
    29641 gcagtggcag catgccgata tggctccgat gcgctcgatt gagctgcgtc ggagcgccgt
    29701 ccgggcggcc agctccggca taacccaaat cattgacccc cgtggccggg tcatagctca
    29761 gcgtactcgc gatgaaggtc ccggggccgt tgtcgcttcg gtgcctctgt gcaaccggca
    29821 aaccttcttc atgcgaggag gacacttgtt tgccgcggcg gtcggctggt cctttctgct
    29881 gacattcatt gtgcttacct tatggcaatg gatccgtgga ctcatttacc tatcgggcag
    29941 gggttcgcac aaaacgtgat gccgctgtcg gcaggcaggt tacaggcggg cataccgccg
    30001 atagaacatg gtgagccggc tgaagttgcc gaatgaggtc cctgacgggg tcggcttggg
    30061 ctttgacagg ccgcagtcaa ccctatagac tgccttgtct tgatcccttc gagcagcgtg
    30121 tgtctgcatc agatcacagg ccgaggggcc tttgtcaggc aatggactcg ttgagggagg
    30181 tttgacatga gcagaaccat gaaggtcgtt tcgttgattg ccatcgccgt gtcggtgttg
    30241 gggattgtcc tggtcgccgg gtgcaaggat cgaggcgccg gcggcaaggc caagccgatc
    30301 gaaatgaact acagcatctt cttcccggac gtgcacatcg tatgccagac ggccaaggcc
    30361 tgggcggagg agatcgaaaa gcgcactgat gggagggtca agatcaagat tcatcccggc
    30421 ggcacgctga ccgctgcgga caaatgctat gatggcgtgg tcaacggaat ctccgacatc
    30481 ggaatgagtt gtttcgcata tacgcgcggg aggttcaagc tgctcgaagg actcgacctg
    30541 cctcttggtt atccgaacgg ggtcaccgct actcgaatag ccacggaact gacgatgaag
    30601 tatgagcctg ctgaactcag cgacgtgcac gtgctttacg tccatgctca cgggccgggc
    30661 atcctggcct ccaagaaggc ggttcgtacg ctggaagaca tgaaggggct gaaggtccgc
    30721 gccaccgggc tgtccaccaa gatcgtcgag gcccttggcg gcgttcccgt tgccatgcaa
    30781 cagggcgaca cctacgaggc cctccaaaag ggcgtcgtcg aggcgacttt ctgccccatt
    30841 gagacgctga agggctggaa gcagggtgag gcgatcaact tcgtcaccga tacctcctgt
    30901 atcggttaca cgacagcgat gtttgtggtg atgaacaaga aaacctggga caacctggcg
    30961 gaagacatcc agaaggtgtt taccgaaacc agccgcgagt gggtgttcaa gcaaggcgct
    31021 gcttgggatc aggccgacca agagggactg gctttcgtca agggactggg gcgtgagatc
    31081 attactctgt cccccgagca gcaggaaagg tggaaggagc gcgttcagcc cattatcgat
    31141 gagtacgtgg ctgcggccga ggccaagtcc ttgccgggtg ccgcgtttct caaggacatc
    31201 cagatgatga ttgcccaatc ggcggcgtcg aaaccagaat gatgggcgcg gtgcaaccga
    31261 tcatttcgaa gctttccggt ttctggacgg cgtacgctcg cagcgtcagg atgctggtct
    31321 atgtcctgga gatcatcgcg gccctgggca ttatcacgat gatgctcctg acttgcgcgg
    31381 aggtgatcct tcgcatcttt cgcatttccg tgacgggcgt ggttgacgtc gtctgtgtcg
    31441 cagcggccgt caccgcggcg gcagcaattc cgtatacgac ggcctgcaag ggacacgtcg
    31501 cgatcgagta cttctttcag aaactgtcgc ggcggggccg aatcgtggtg gacacccttg
    31561 tccgcatctg cgtcgttctc ctgttcgggt ttttgaccgt ggagtgcgtc agttacggca
    31621 tgtcgcttaa agccaatggt caggtgaccc cgacgctgca gatgcccgac ttttgggtgc
    31681 cgtacgttct ggccgcatct tgtgcggttg tgtgcctggt gaccatttac catttgtttc
    31741 atccaggcaa ggaactgatt aagccatgac gcccgtcgag attgggatac tgggttgtat
    31801 cgtgctggtg ttgctgctgg tcagcagcat gcccgtggct ttcgccatgg cgatcgtcgg
    31861 gtttgtcggt ttcgccgtgg tcgtcacacc tcacgcggct gtaagcatgg ccacgaccga
    31921 gctgtgtgac actttttcca agcacagcct cacagtgatc cctctttttg tgctcatggg
    31981 ccaggttgcc tttcattgcg gcatcagccg ccggctcttc aacacggcct accattggtt
    32041 tggaccgctg ccgggcgggt tggcgatggc caccgtgggt gcttgcacgg ccttcggggc
    32101 catctgcggg tcggggcccg cgaccgccgc caccatgtcg ctggtcgcgt tgccggagat
    32161 gaagcgatat ggatacagca tggagttagc ctgcggaacg gtcgcatcgg gcgggacgtt
    32221 gggcatgatg atcccgccga gcgttgtttt catcgtctat ggtatcatga ccgaacagtc
    32281 gattggcaag ttgttcatcg cagggatact gccgggcctg ttgaccgcgg gcctctttac
    32341 attattcatc tatgtcgagt gcatccggcg tcccggttac gggcctgccg cgccctcaac
    32401 gagttggggt gccaagtttg tttcccttct gggtgtttgc gagacccttg cgctgttcct
    32461 cctggtgatc ggcggcatgt tcgccggttt cttcacccct accgaaggag cggccatcgg
    32521 cgccggcggc acgattgtca tcgcgctggt caagaggcaa ctggccgcga agatggtctg
    32581 gcgagcgctg caggaaacga tgcgcacaag cctgatggtc atgatcatcg tggcgggtgc
    32641 aatgattttc ggcaagtttc taacggtcac gggcatcccg accgaattgg ccgcgtggct
    32701 gggcggtctg ccgttgccgg catgggtgat tgtcggtctg atcatcacct ttttcatcat
    32761 tgccggatgc tttgtggatg ccttggcgtt gatattgctc acggttccga ttttcaggcc
    32821 gatcatcaac ggattgtatc ccgaacagga ccccatgctt ctgatctggt tcggtgtcat
    32881 cattgtgctg gtcactcaga tcggaacgat taccccgccg gtcggggtct gcgcatacgt
    32941 ggtaggcgga atcgagcgcg acgtgccttt gcaggctgtg ttcaagggct gcgtgccttt
    33001 cgtgatggcc ctggtcatcg ccaccgtttt gtgcgttgcg ttcccgcaaa tctgcacatg
    33061 gctgcccggc atggttcgct aagagagcaa ggatccagac catggatagc ggcacatttc
    33121 cagtcagccc gtatcagaag attctcttct gcaccgattt ctccgaaaac gccgattatg
    33181 cgttcaactt tgccgtggac gccgccgtac gccggccggg ctgtacgctc tatctgctgc
    33241 acgtcatccc ggaaagcgac gcccagttct ggaagaccta catctacgaa gttgacggta
    33301 tagacgagaa ggccaagcag gatatcgacg agaaaatcaa ccttgattac aggccccgcg
    33361 ttccggcggg cgttgatttc gaggtggaga tacgcgtcgg caaggattac caggtgatcc
    33421 tggagtttgc tgaggaaaga gatatcgacg tgatcatcat cggtcgccaa ggcagaggcg
    33481 gtctgcaaaa ggccttgttc ggaaatgtca ccgagaaagt cgcacgcaag gcgccgtgtg
    33541 ccgtgttgat catccccatg gacttccaga aaaagcaccc acggtaactc gattcagtgc
    33601 agttccggag catcatgcca tgatcagacc gattcattgt cggctcattg ccgtttcagc
    33661 ttgtgtttga ggcaccgttt gggttgccga gacgcagcgg aaccatgtct ccactcgggg
    33721 caacgatcac tggtcaggca agccgtcaga gcccgccggc gacggcaacg atggcccgtt
    33781 ggccacgttg acgcgtgcgc aggaagcgtt ccgagcgagc aataaagccg gattgaaccg
    33841 gcctgtcgag gtcatcattg gagaaggcaa ccaccggctg gccgagccgc tcgtgttggg
    33901 cccggaagac tcggaaacgg agtcggcgcc gatcacctgg caagccaaag gcagagtgat
    33961 cctgagcggc ggacgggtca tcaccggttg tcgcgagacg acgcctaacg gcaacaccga
    34021 ctgagcggcg gaaatcccgg aaggaggaat gaccggttcg agacggacga caatctttat
    34081 ttgcgagtca acggccagcc gttcggtctt gcaggagtga cgctcgaagg ctggggccag
    34141 cgcggccgcg accgcagctc gatcgtcgcc gacccgctct tcgttggttc gagttaaggc
    34201 gacttcactc tcaaatccgg ctcaccgacc aacaaggtcg gctttttgcc catcaatctt
    34261 ggtgcggggg gacgggtgac gtggcgacgg caccatgagt gcaatagcct gcaccgtgca
    34321 gccctatggc catccgggca aaacgcgcag gtttgagatc cgacgtatta tcggacgtag
    34381 aaacctctct ctgcgccccg tttcccaaac acaactggca cctcagtgag accccggagt
    34441 ttccgataat tgtggcatgt cctttattga aacgcaacga aggcgggcgg ctcggcgtcg
    34501 gcatgctccg tcggtttatc gttcagctgc tcgcgtgggt tgcagtgccg gcacatcacc
    34561 cggttccaac gatgcacgtc cctgggtcat tccggcgaat ctcctggggc aaacagacca
    34621 cgagcagttt gccggtaatg acgacgtgct gggaagagaa ctcggcgttc ggccacgact
    34681 actcgccaag gccctgacca ttcgccgaac cgaggaacgc ctgctcaagc tattcgccga
    34741 gggcagagtc tcgggcaccg tgcacacctg cattgggcag gaatgggtgg gcgtcgcggt
    34801 cgctgatacg ctcaaggagg gggacttcgt tttcagcaac catcgttgcc atggacatta
    34861 cctcgcgtgg acgaacgatg ttgagggtct catcgccgag atcatgggac gcagcaccgg
    34921 cgtttgcggc ggcagggggg gcagccagca cctgtgcaag gatgggttct tctccaacgg
    34981 cattcagggc gggattgctc cggttgcggc cggactggcg atggcccgac gacttgatgg
    35041 cggcagcaat attgccgtgg ttttcatcgg agatggaacg ctcggacaag gcgtcgttta
    35101 cgaaacgatg aacatcgccg ccaaatggca tttgccgttg ttgatcgtcc tcgaagataa
    35161 cggcgtttct cagtccaccc tgcaggacga aacgctctcc ggctctatca agggacgggc
    35221 gcacgcattc ggtatcgacg tgcgatccgc cgacacctgg aatccgtccg aactaatcga
    35281 tgtgaccgcg gcagcggttt ctcatgtccg tcggacgcgg caccccgtgc tcctgcacat
    35341 tcgtacgtac cggctgaagg ctcactccaa gggtgacgac aatcgtgaga tcgaggaagt
    35401 tcaggcctac gccgagatcg atacactcag tcgcttgctc gacgagcctt ccgatccgat
    35461 caggaacctg attgagaaga tcgaccggag actcgacgaa gccgttgacc ttgcggagca
    35521 ggcttcgccc accgagccgg ttgttctcct ggatcatgct cccgccggaa ccgagcctgt
    35581 cgaatggcgt tctctcgatt ttcgcccgca acgcgtcgtc gacgcgatcc gcacggcgct
    35641 cgatgaatca atggccgagg atgatcggat tgtcctgatg ggtgaggatg tctgcgaccc
    35701 gtacggcggc gcgttcaagg tcaccgcggg actgagcagc aagtatgacg atcgtgttcg
    35761 caatacgccg atcagcgagg cggctgttgt gggcatcggc agcggcctgg ctatcgcggg
    35821 tttcaagccg gtcgtcgaga tcatgttcgg tgatttcgtc atgctggcag ccgatcagat
    35881 catcaaccat gcggccaagt tcgctgaaat gtacaacgcg caggtgcggg tgcctatcat
    35941 catccgtacg cccatgggcg gctatcgggg atacgggccg acacacagcc agtcgctgga
    36001 aaaacacctt ctcggggtgc ccggcactcg tgttctggcc gttcataccc gctattgtcc
    36061 cggcaccctg tatcgccgac tcctgcagac catcgatcgg cccacgctgg tcatcgagaa
    36121 caagttgctc tacggccggc gagccgatcc gcaagccccg gccggatcgg tcatcagcgc
    36181 gaccgcgacc gactttccga ccattcgaat ctcgccggag agcgagccct cgctgacggt
    36241 cgtggcctac ggcgggctcg tgccgatggc ggagaccgcc gtcgagtcgt tgatcgacga
    36301 ggatatcgcc tgtgacatgc tgatccccac tcaattgtat cccctggaca tcgagccgat
    36361 cgtcgagtcg gtggcccgaa cggggcgtat tttggtaatc gaagaaggcc agggctttgc
    36421 cgggttcggc agcgagttga tcgcaaagtt ggcagaggac cgccgcattc cgtcgctccg
    36481 ggcccggcgc gttcacgcct ggccttgtgc gattccggct tcaagcactg ccgaatccac
    36541 gatcctccca agcgttgaat cgatccttaa ggccgtcttc caacttgtgt tgggttgatt
    36601 gctctcaaag ggtgtgcacg cgtcatcggg tcgcgttggc cgatgttggc gtcgtgttcc
    36661 cgtcgttgag gaagcagcgc gtcgttgcgt agcctgagcc gtcacggaaa gccccgtcga
    36721 aacggatctt tggtgcggag acgctttgag aaggcgaatc gacagcggaa ttggagaaat
    36781 ccgcgggatc tcgggcatac gtctattgcg tgcggctgcg atagcagacg accctttggc
    36841 tcaggttgtt ggcattgagc tgaatgtgtc ctctgccgcc gaggtcggcg gtatcgatga
    36901 cccggatgac gtgggcccaa ttggccgccg acggtatgag tcttgtcagc ttgttcggag
    36961 ccttttcgaa gaactcctca ttaagaaagt tgccctcgcg gccggggaag agagcaaggt
    37021 agagaatatc cagctcgacc aactcgttga agaagtgcgt gccgagagag acgtcgggga
    37081 ccaggtcttc gcgcatggcg acgatctcgc agagcaccga aacggtgttg atttcagcga
    37141 acgagacggg gacgcccagc gagggcgtag tcgtgcccca gcggccggga cccaggagca
    37201 tgatgcgctc gggtttttgc tcctcgtcgg catgcatgag ccggccgatc agccgggcca
    37261 ccgcgtagcg ttcgggaatc gggaggtgcc cgtaaaccga ggggacaacg tagacaaggc
    37321 gatcgatggc ggcggcacgg ctctggccga tgacggcgcc gtgagcttcg atgacaaggt
    37381 ccttgggatc gatactggcc ggcatctcgg ccgcctcatc cgcccggccg ccggcgactt
    37441 gcagcggcct gcactggacg agattgatct tgtattcact atcttcgaag aagttggccg
    37501 taaactcgat gtccacgggg caggcgtagg ccttctccag cgtggcgacc atctcgcgca
    37561 tgtcggacac gaaggacgtt tccgacaaga ggtggtcgaa cgtcaggatc catgggaaga
    37621 gatccttgcg tcccgtctcg gcggccatac gttccagctc actgtctcgc gaggcgaaga
    37681 tctgcaacgg aatatcgttg ctttggcgga tcacgtcgac gaagtcgttg gacacaagct
    37741 ggtttgcggc caggtcaagg gcgtcgacct tgcgctgcgt gtagcggcgt acctgatcga
    37801 tgcccgcctc cgggcggcgc ccaggggcgt tgagggcgac cactcgggtg taatcgtcgt
    37861 ccgagcggtc caccgcccga gtgcccaatc cgaaaaccag gcgcagcatt cctgccttag
    37921 ggtcgatgtc ctcgtgccac acatacgggt tgtacgacaa accgacaccc gccacttggg
    37981 gatagaagag gttgccgtgg agcgatccgg acacgcgttg gacgagcagc gacatctgct
    38041 cgtctttttc gagcaacccg cgctgagctc tgtattgcag ggccttttcg ctcatggtgc
    38101 tggcgtagat cgtccgcacg gcggacatga aatcggcgag cctcttgtcg cgggatccct
    38161 ggttgacgca gaacaggctt tcatatttgc cggcaaaggc gttgccgtag gcatcctcca
    38221 gcaggctgct tgagcggacg atgatggggg actgcccgaa gtagtccagc atttcggcga
    38281 attctttttg cgtttcctcg gagaagctcc ccgtgatgat gcgccggcgg gcttcctcag
    38341 aaccttcaag gaagttctgc gggttcctct gtttctcgcg aagccaccag acgccgttct
    38401 gtaccaggta ggtgtagaag acatccgagc cgatgtagaa cgaatcgtgg acttcgagtc
    38461 gccggctcca cttggggtcg ctctgttcga ggatggcccg tgacaggagc atgccgaccg
    38521 acttgccgcc gatgaggccc gttccgaggg tgcgccgtcg gatctccagg attctggcgg
    38581 ggttgaggta tttctcggcc aggggcagga tccgctcatc gcgagaaatg agcatgcgca
    38641 tgaggcggcg gtggagatgc ttcttctcct ccagggaaca ggctttctgg agcgacgggt
    38701 cgaccagctc ctcggcctcg cggaaagccc ttgcccacac gcccaagcgg gcgatgcctt
    38761 gttccgtgct gccccagggc cgcgaggtga gaatctcagc gatggtcgag ctatcattga
    38821 ccggccggaa ggtctcatgt tgtggttccc aagcgtgcag catgtacatg gtcggcgaat
    38881 gacgctgttg taccttgatc gggtgcaggt agtagcggtc acggtgccgg tagatgtcca
    38941 cgaggatctg ggtggtgttc gcgatggtcg ccacggcttg cggcgaatga tagttcttca
    39001 gcagggggaa gtaggcgatt ccctcgacgt cgtacacgta gggacaggtc agcacgaaga
    39061 agttgctgag catccggtcg ctgctccagt ccaccgccaa gtcggacaag cagtcaaaaa
    39121 catgcgcccc caagccgcgt gcggacttca cgacttcatg gatggagctg atgaacttct
    39181 cgaagccgag ttcgggctgg agctcatgaa tctccgccgg gcaatcggcg ggcaccagcg
    39241 gggcatgatc ggcgaagcgg taatagacca gccgcttgcc ggcggacacg gcgttacggc
    39301 agaagggttc gaggaagacg cggtagtcgt ccaccgtgtc gacgcgccaa acgagattgt
    39361 cgccgggcag caagcccttg aggacacgat ccagagcggg taagcccgtg ctcagattcg
    39421 tactgatcat tcacacttct cctgcgccaa atccggctcg ctcacggtga cctgtggcaa
    39481 ggcaggcgct tgagcggtcg cgccggatcc ggacgcgatt cgtcagtctg cattcgccgt
    39541 cttcgccgca tggtaggcca aattccgttt tctggcgaga gatccggttc ttgccggcag
    39601 gcacggcggg acccgccgca ctgtcataag atcgacttct actgtgtatt agggacatca
    39661 gattatgtaa tcccgtttct cggcggtatc atggttcctg gcctgcagag cgttgagcat
    39721 cctgcgattc agcgctatta taccgctgct gggacatgcg cggaacacgc cgccggacag
    39781 gtacgtcccg gcgtggggaa cagagggcgt cttggttggg agctggtgag gccatgtttg
    39841 tcattcagga agcaaaatgg cttgccccgg cggtcaggcg gctggtggtc gaggcgccgc
    39901 gtgtggctgc ccatcacaaa cccggtcact tcgtgatcgt tcgcgttgtt gagggcggcg
    39961 agcggattcc gttgaccata gccgattctg acgaggccgc gggcaccatc acgctggtcg
    40021 tgcaggccgt cgggacgtcg acccagcggc ttgcagcttt ggagaagggg caagccatcc
    40081 tggacgtggc gggcccgctg ggcaagccca cggagattaa gggattcggg catgtggtgc
    40141 tggtcggcgg gggtgtcggg acggcggtga tttatccgca ggccggggct ctcaaggcga
    40201 tgggcaacag agtctctgcg gtgatcggcg ggcggagccg accgtacgtg atcatggagc
    40261 aggaactgaa ggccgtttgc gacgcggtat atccgtgcac cgatgacggc agctacggct
    40321 atcacgggct ggtcacggga cgactcggcg agttgatcga cgacgcatcc gatccggtca
    40381 acgcggtgat caccgcgggc ccggttccga tgatgcgggc cgttgccgaa gtcacacgtc
    40441 ccaaagccat tcacacgatt gcctctctca accccatcat ggtcgacggc acaggcatgt
    40501 gcggcggctg ccgcgtgatg atcggcggga ggatgatgtt cgcgtgtgtg gatggacccg
    40561 agttcgatgc ccaccaggtc aacttcgcgg agttggccga ccggctgacg gcttatcgcg
    40621 attacgagcg aaaggcccag gcgcgcacgc ccacgaacca tgagtgcaag ctgatcgagg
    40681 cggagaaggc gatccgccgg gaggcgcccg tcgcttgata tcagggcgcc attcttgccg
    40741 gtatagtcga aggtcgataa gtcatgcagt tgactcccgc agaacgaatg aagattcccc
    40801 ggcagcagat gccggagcag aagccggaga tgcggacccg ttgctttcag gaggtgaacc
    40861 agggtctggc acttgaagcg gcgaggatgg aggccgaacg ctgcctgcga tgcaaggagg
    40921 gcaagtgtgt ccagggctgc ccggtgggcg tcgacattcc aggcttcctg gtcgccctgg
    40981 ccgacggaga cttgaacaag gccgccgaca ttctttacga ggccaatgtc ttgccgggca
    41041 tcaccggccg cgtctgcccg caggaaacgc aatgcgaatc catgtgcatt cgcggcaaga
    41101 agggttcgcc ggtggccgtg ggttacctgg agcgttttgt ggccgattgg gctcgcgaca
    41161 accggccgga tcagtatgtt ccccctccgc cgaccggcaa gcgggtagcg gttgttggcg
    41221 cgggcccggc ggggctgacc tgcgccggcg aactggccag gaaggggcac ggcgtcaccg
    41281 tttttgaggc tctgcacaga cccggtgggg tgctggttta cggtattccc gagtttcggc
    41341 tgcccaagaa gatcgtcgag gttgaggtgg acaaactgcg gaagctcggg gtggagatcg
    41401 tctgcaatgt agtcattgga cggacccata cgattcgcga gctgatcgag caggagggct
    41461 tccatgcggt gttcatcgcc aacggcgccg gcctgccggt cttccaggga atcccgggcg
    41521 agaacctcaa gggcgtctac tcggccaatg agtacttgac ccgggtgaat ctcatgggtg
    41581 cctaccgcga tgacagcgag acaccggtga tccggtcgac agaggtggtg gtcgtcggcg
    41641 gcggcaacgt tgcgatggac gccgttcgca ccgcccggcg gctgggtgcc gatccggcga
    41701 cgctggtcta tcgtcgctcg cgagccgaga tgcccgcccg tgtggaggaa gtcaagcacg
    41761 ccgaagaaga aggcgtcagg tttgagttcc tggtcaaccc agttgagatc ctgggcgatg
    41821 acaagggctg ggtgcgggcg gttcgctgcc aacggatgga actgggcgag cccgatgcca
    41881 gcggccgtcg gcggccggtg ccggtgaaag acagcgagtt cgacatccct tgccaggtgt
    41941 tcatcgaagc catcggcacg aaggccaatc ccctgctgac ccaatccacg cccgagttga
    42001 agctcaacaa gtggggtaat atcgagatcg acgccaacaa catgaccagc attccgggcg
    42061 ttttcgccgg cggc
//
LOCUS       DAOTPW010000011        40026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1099_length_40026_cov_4.978384, whole genome shotgun sequence.
ACCESSION   DAOTPW010000011 DAOTPW010000000
VERSION     DAOTPW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 40026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40026
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_40026_cov_4.978384"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="P5316_01765"
     CDS             complement(<1..560)
                     /locus_tag="P5316_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83712.1"
                     /translation="MPAEMAEDSSKRTHAAACLTMERPDTLARTASLVGTVLLIWLVT
                     AFPLHGQPRPLLPIDDAQAESTLLPAGIILAKPEIFAGYVYLRPEPDGTQVLQFHGDF
                     SLHVGARELKSQEAVVWMQRCQWEGQSFYHYEVFLSRHAQVRDSADTITSGPTLFVTF
                     NASKPVEIYNDVHTTESPEDTKLFQDA"
     gene            complement(732..2309)
                     /locus_tag="P5316_01770"
     CDS             complement(732..2309)
                     /locus_tag="P5316_01770"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRR83713.1"
                     /translation="MAESNNKWLHFLLVMVVLVLVGMYFHEQIIGSVAYAVEKGVREA
                     DKEHLAKVEAISQAFRLVAKQVKPTVVHITTTAKPPERRGRRPQIDPKQIPEPFREFF
                     EEFQDPRLLPQRPREGTGSGVIIDAEAGYIITNNHVVSDVEDGQGRIDVRLPDGRKVK
                     ADVVGRDDKTDLALLRINADKLHAITIGDSDAMEVGDWVLAIGSPFGLDQTVTQGIIS
                     AKGRSHLLEIPYEDLIQTDAAINPGNSGGPLVNMRGEMIGINTAIATNSLTRGYMGVG
                     FAIPSKTIKEILPSLKEGKEIVRGYLGVRIVSLDAYGPGFGKTFGLDHDDGVLVEDVF
                     EDTPAAKAGLKMDDVILSYDGKPTKEATQLTMMVTKTSPGTKVDLKVWRDGKEIAIPV
                     TIEKQPRDFFTWGGRRTEEGDTEKEDRKSGPAEIAAIGITVEALNEELAKQYGWDEEY
                     DKVKNLLVITEVDPIGEAHAQGMRPGSLIISVQGKEVRTVAALKQALSQEALARGVRL
                     RVRTIAGYRTFFLQVEK"
     gene            complement(2617..5517)
                     /locus_tag="P5316_01775"
     CDS             complement(2617..5517)
                     /locus_tag="P5316_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83714.1"
                     /translation="MASLELPTNSPQPLREWQRRLLVSSVIILSPAVILAGAWRLGGV
                     SALEDDLIYYLPVRQYIGERITAGDWPLWNPFSRMGISIAADPQSGLWYPPTWLFAVL
                     PPLVAYPVTIILHFALAGMGMYRFLRSLGRYWQAALFGAIAFEITGYLVAHRAHLTIH
                     HAAAWLPLIFYEWQRFSTDGRYKHFARAALALGAQMLVQHVQVTIIGLALLSGYALVV
                     LRPRRPRLMWEFPLGVAAGILVSAIQFLPTLLHLSATGRATPSYATFVENSWTPLSAL
                     LLLFPMLFGNRTPNLWHQPWWGISHFCEQSAYGTILILVLALASYWLLRGHQRPSVRL
                     ASLRAQGTELQRASSRPWNREILFWWIASFVVLLLALGELTPLAKLLFRVPLYRSLRV
                     PARWILGWSLAMPILASAVISAVLSSQPAADKATGGIRYVVKRILPITFAICVLLMVT
                     ARWQVNSLESAFAGRWNADVFFSGLRAAIRPGNPAIWWPILLAAATGALLLRWVDRGR
                     THQLVLLFLVMMVDAASVAAFIDVDVRTYQRSDLQNPPELARAIAATKPDDGHRLLVP
                     RFSADYNRPIEMLWPQTNVQRDISTVHGYGPLWPLSSRLLLRFMPWGSSQEMLSLLRD
                     PRLLKSLGVRFIAVRSRQERALVEAALWPDAPAPNLSLIDGTELPVPVRAGHDLLWPV
                     NIPSAGIYELEIDATPTPGESNRWFVRLETQNVEQIEEIRMLTPEDLAVGPRRMRFQF
                     RCDQAYGPIRVRVKAEYGQPVTVSHAAFGRAAAPPQTISTAPAATEARSPWNHVTDLP
                     GDISLYELRNASPLLFWAKRVTPATDLLQAVKLLQARPSQLEDPTHVVVEDLGQPVFA
                     EAPGSAASLQWRRPSAEELLVDTSSTVAAFLVFNETYDDGWTVEIDGLVSRPHRVNAL
                     VQGVAVPPGRHRIIWRYRPVGLSAGIGLTLLSLLTLLVPLAARLRRPSVRQH"
     gene            5714..6205
                     /locus_tag="P5316_01780"
     CDS             5714..6205
                     /locus_tag="P5316_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83715.1"
                     /translation="MDSDDETVKGVILTARIISGAMIAGVATFGVVVLLLGNVVASQP
                     SLGGLFMGILGLIAPAFLVAYVVLRGVMVRAARSQCEQSGGVGMSLEALARAYAPLVI
                     IRAAMAEGVGLAGLVFLLITGQKLLWVAPVVAIIILVAALPSRSRFDSFVQEVTGTNP
                     YLG"
     gene            complement(6295..6516)
                     /locus_tag="P5316_01785"
     CDS             complement(6295..6516)
                     /locus_tag="P5316_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRR83716.1"
                     /translation="MIPMGKLIIVLGIVMVVIGAAIWALGRLGFRGLPGDIHYESENV
                     HFYFPIVSCIVLSVILTLGMWLWQRLSQR"
     gene            6693..7931
                     /locus_tag="P5316_01790"
     CDS             6693..7931
                     /locus_tag="P5316_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRR83717.1"
                     /translation="MRALTYSVRPVQWTVCKVMGWLWPSVFWSRLSGLRLSEVPVPTL
                     PGPRWVRLKTILGGICGTDFSGIMQRGHPASILQAFSSFPALLGHENVALVDAVGPAV
                     SDWKPGDRVVVEPTLSCVPRGIDPVCRNCAAGRFTLCENFRAGPLPVGSMIGWNCFTG
                     GSWGPFFVAHESQLHRVPDEIDDRIAVLIDPMAGALHAVLRRKPWEHETTLIVGDGLL
                     AMGVAACIRALGSRCRLLAIGLWETLRETMQRFGVDEFIAVGGRNTQAARYGKVAARV
                     GGKLVATKFGHQAFIGGVDLVYDCVGTGQTLTDSMKYARSGGTVVEVGTSQISLVDTA
                     PLWFDELTLMGTNGRAIEQYDGRTMHTYEIVIELIRERKLDLSGLLTHRFRIDQYRQA
                     MAALADRARSGAIKVAFEHP"
     gene            7965..9044
                     /gene="waaC"
                     /locus_tag="P5316_01795"
     CDS             7965..9044
                     /gene="waaC"
                     /locus_tag="P5316_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HRR83718.1"
                     /translation="MDLAAREYRRILIIKPSSFGDVIHAMPVLHGLRTRFPRARISWL
                     VAHSCAGLLEGHPDLDEVIRFDRKRYGRIGKGLTPTIEFVRFLGDLHRRSFDLVLDLQ
                     GLFRSAFLTLAAGAQVTLGFANAREFAPLFYGRRITVPDPEMHAVDRNYLFSRALGFA
                     DLPVSFNMPVRSEAAAAVATVLADHGIARGHPYALMGPGTRWETKRWPAEAFAEVARR
                     VRAELGLPVVLIGMEDEAGVAWQVEQAAGEGIVNLAGRTKLPELIALVAGAALCVMHD
                     SGPMHLATALDRPMVAIYGPTSPRRTGPYRRPEAIVRLDLPCSPCYIKRVADCPYDHR
                     CMRKLPPDTLMDRLRLSYPPGVNDR"
     gene            9041..10498
                     /locus_tag="P5316_01800"
     CDS             9041..10498
                     /locus_tag="P5316_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83719.1"
                     /translation="MRRMSVTVLPVLLLALTAEGQVSRATPVTPVLVTSSGEYDAERQ
                     LVTLGVGRDEGVGVGDHFWLFHQENITGTGTVYLVSGTQSVGRLAFTSRNVGAKQSAV
                     VLVGASLTALRPQVSSEVPIRSRVVRVPPARRTAWLDFGRRSGLQEGDPVVIRRFNLP
                     IARGQLEIVEDEVSLAALHPLVGNALPEPGDDAEIWPPPGVDLQVRLKSTILDVERSS
                     EGPVITFVGTAADGIVENRLVDIFRKEQFLGVAAVREVYEPLSRALVMEPATRGMPEV
                     GDTALVRLRPGQPPKPLTAVVFKVAGDYCLIAAGESDGVQQNEKFVVRGPIPGGMGGM
                     REIAELTIEAVKVDYSGARIRPLAADGVTVKAWDMAERRTIHERSEWIYAGRVDLTHT
                     DSRTLVAGAEPDVALTGGQVVRWTPPQATGGQPGAAVVIYVGRDKTFLFVPPCWGNLA
                     EAPGARVEIPASELSPSASDEVSSTRPAIQTTRPE"
     gene            complement(10547..11107)
                     /gene="thpR"
                     /locus_tag="P5316_01805"
     CDS             complement(10547..11107)
                     /gene="thpR"
                     /locus_tag="P5316_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRR83720.1"
                     /translation="MRCFLAIELPRETRERLAALQKRLCALDREVRWTKVDQIHLTVK
                     FLGEVPDAAVGQVCQSADAIARDHPAFDIQVSGTGCFPPRGPARIVWAGLASLPQPLI
                     DCHKACERAFAEMGFPPENQPFHPHLTIGRVRDQRGSHKIRPALESELHFDGGRFTVK
                     ELTVFQSVLDRGGPTYTVLSRAAFQE"
     gene            11287..12249
                     /locus_tag="P5316_01810"
     CDS             11287..12249
                     /locus_tag="P5316_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR83721.1"
                     /translation="MNESLDRRGFLQGGALAAAAMAVGGDVGITATKAAATEDKMNTK
                     AVLRLSSGEGIIPGNSLTEKLDKMEKWGFEGLEVGGGYLRSNVKQLKEALKGRAVRMS
                     AICAGFDGWLIADDPAIRDKAMTTMKELLTMAGEVESTGLIIVPAFNNQKSLPHQQAR
                     ELLTGFKRWDAPAAERKAGLLQELGDHASKAGTRILLEPLNRHECYFLRTLADAASIC
                     KDVENPGIAMMGDFWHMTWEETSDCGAFLSAGKYLHHVHIASRRERKTPGEDGAADNY
                     VDGFRGLKMIGYQDFVSLECGTKGDKEKVLPACVALLREQWEAA"
     gene            complement(12328..12678)
                     /locus_tag="P5316_01815"
     CDS             complement(12328..12678)
                     /locus_tag="P5316_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83722.1"
                     /translation="MDTLEQEIMNTERIIAGLPEVQAWALDRSLRTLTPAQKAYVTES
                     LRSLEADDTVSPRYLQLLEHFFDKWSSHNLATKLVLINRLTRLSARDVTGGSLPAGLD
                     HHVFTLPEILQEVT"
     gene            13087..>14451
                     /locus_tag="P5316_01820"
     CDS             13087..>14451
                     /locus_tag="P5316_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRR83723.1"
                     /translation="MKRSLLQVLSMMIWASQVAVAQEDRYAVNRGLPGDAMIQTYLEH
                     AAARISGRFLEGTDSLENWKAMRPRLRQEYLYMLGLWPLPERTPLNPVIRKTIDGQGF
                     AVDLMHFQSRPGLYVTANLYRPAIVEHGRRLPAVLYVCGHSSRGRDGNKTAYQSHGIW
                     YARHGYVCLIVDTVQLGEIAGIHHGTYREGRWWWHSRGYTPAGVECWNGMRAIDYLVS
                     RDDVDPARIAVTGISGGGASTFWIAAADERVAVAVPVSGMADLPCYVGQRLINGHCDC
                     MFLHNAFQWPWTYIAGLIAPRPLLFVNSDQDPIFPMSANERIINRLERLYSLFGTSDR
                     VDAVVSIGGHEYRKDIRQACYRFINTWLKGDPGLVEDSEVDLVSREEDPSHYPIPASN
                     LRVFPDDSDIPADQINTVVDRVFVPMAVPEVPSAHRLNAWREQLVRELRRVSFSGFPE
                     RIPPA"
     assembly_gap    14452..14461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14462..15085
                     /locus_tag="P5316_01825"
     CDS             <14462..15085
                     /locus_tag="P5316_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83724.1"
                     /translation="RDGQGRLRLATEQGIECCAMPVGVVPEMVRQVLLVVAGREEEVV
                     VPAVIETLKQDGEVVFRCEPRGVGGTRWTRKNPPNYVERAHVLLGRTVDAGRVWDVVA
                     TARFLAMDRKVPVRVGGVGAGGILAAYAALLEPEIGGAAVSGVPASHMDEGAPQLLNV
                     LRVCDIADVLGMVAPKPLRLRAVAPSVSEKVKAIYAAAGASDRLLIE"
     gene            complement(15141..17072)
                     /locus_tag="P5316_01830"
     CDS             complement(15141..17072)
                     /locus_tag="P5316_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HRR83725.1"
                     /translation="MEVRIWGDEYHQIVESLDGFALVRDPTSGAICYAKPADGKEDLA
                     SSGVVVGHPIPQSLALKRHLRPSAAAIRNRVSAARAAFSAPASAGTPQVAGTGEPVPA
                     TTPSGRVEGICLLVDFSDDPGTIPPQTVWNFCNQIGYNGFGNNGSVRDYFYHVSKGHL
                     TYTNFVPGQYYRASQPKSYYDNPNYRCPTRAQELVIEALNALDAAGLDFSMYDADRDG
                     QIDAINCLYAGTTSAGWAYGLWPHCAPISFSADGVTASRYQITDMDGALYLDTFCHEN
                     GHLVCNWPDLYDYAGSSSGIGKFCLMCDMASRTNPVQPCAYLKYIAGWTQTTLLVSHQ
                     VGLSISSDQNQVYKYSHPTKPNEYYLIENRQQTGRDTALPDAGLAIWHIDTYGTNTTG
                     DPGNNEVTLVQADGRWDLEYGANQGDGTDLWKSPTYTACGSHTTPSTKWWDGNASGLG
                     LSQISNSGSVMTFTFAAAAIVLDTQEIVHVIYPGRAVPDDTFTVTNASMIAPMDYTIG
                     CTAGWVTISPSAGSSMGEADTITLTYRNDLIQGWQSGSYGAIVFVGAENAWNSPQAIS
                     VQVIIGGVAADLDTDTDVDQADFGLLQACMSGFGIKQTNPDCAKADLDKDGDVDHDDV
                     AVFMGCLSGADVPADLNCE"
     gene            18272..18622
                     /locus_tag="P5316_01835"
     CDS             18272..18622
                     /locus_tag="P5316_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83726.1"
                     /translation="MDCVLVGVYPPNLSPRALRSLHRLLKRDNRRAKKFCRWLDGVIV
                     REMRRRVRERSGQYVEAELPEIDFCNWSDAEVGAGLVVAAVLVDAVRDRGMAAFMRRL
                     VFLFSIEARERLGG"
     gene            19431..20420
                     /locus_tag="P5316_01840"
     CDS             19431..20420
                     /locus_tag="P5316_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR83727.1"
                     /translation="MAGTKTVTTRAVEAGPAAVRFVLPSEADMARLSATDQAVLRMLH
                     ENAADFVGYMDHPLFAKSNVERTLFGKKQTLLPGQTRFKDASPQDLMKRAADGLTLSS
                     AQEILLFQRFNYSRARVARLMAAAVNRPLSWTELCELLAWAHRAMDAREIIAGVNMPL
                     VLAMAKRTRLASLDYNELVSEGNFALLRSIDKFDCSRGFKFSTYACRAILKSFSRVAM
                     KAARYRGQFPTEFDPFLEKSDYHDRKRDDTASLCVQEIRDILSENRAALNEMEQTVIR
                     ERFALTSGQDGSVRPKTLEQVGQLIGVTKERVRQIQNKALKKIRMTLEDSLAA"
     gene            complement(20580..22859)
                     /locus_tag="P5316_01845"
     CDS             complement(20580..22859)
                     /locus_tag="P5316_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83728.1"
                     /translation="MPPSRLIQSLILLAITTAPAKGQLANLGLFGGEVKDIAACSKDG
                     ATELFIAVAGRHVVYRCGAACWEPVMKGPCTDIQIEADRTLGSAGLLWAVRDGSLLTH
                     EPDAPWDQDGWVQNKSVIRATTLFGDPSGMYIGGDGTVYRTTDGGRMLAPLAAFSNEQ
                     VRSIAVYDKTLFYVVSGKSLYRCLDDGNGFVAGNIQVVHDGTLADEILAVAIDPRDPR
                     NATTASRFFVVCGGKTSSLYRTEDAGATWRRMPVKAPSSQVLGLAFVTFHDRPRIIAG
                     SQYSDDFGQTWGNLPETTIATSLGYVNAYPTGSVATPDPANPNLVYFSTTLAVGQWDM
                     TTARAGEIHNAAGIDGVSVVDVAQIADDPLTYGVVWAATSQGLAKTMAYPDVFDGEWL
                     FPVRPQGQTAALTKVTMHPGDLEVVFAGDNAGTIYRTTSGGRHPEHWAAVFSTRNVPF
                     SNRYSSPEGVAVTAITIVGKDSTLVYAATAVGGGKYEGTVYRSLNNGSSWDDDFAAVA
                     GQELNMPVNDLALLDGMVWAAVGHPQDPRGDAKGLYTRLSITGVAEWRKLLTGTSLDS
                     QAVLAIDGVQVGDVRVLYAATEHGMFCGRLDKTASSAWSWKGISPNGARRYAALAADP
                     GNPSRILVGHDDLLWASFDGGATWGLAPSHPLTPHDRVLTLSYEDLLIGTNYGLFAAS
                     DEAFDPAGRIRTSAAPRRPATQPSGITANLPPVSPQPQPDILPFRLPGVFGLGVADTI
                     GIWLPLLAWLAIARHNRRR"
     gene            23399..23992
                     /locus_tag="P5316_01850"
     CDS             23399..23992
                     /locus_tag="P5316_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83729.1"
                     /translation="MSEQPSLSVRSRRTVSVLLTVLCLVIAAAQIVIVATTYEYANRS
                     VSSREGAVPAEDPAELDRLKSGLMAADPGRRVESAVALLQRGMIEGGSVLLEAASDPD
                     VGARRVAVAEFGRVARPMAEAVGVVLDWPVPADTPPATEHIRLAKAFWERWATPRLLL
                     DVQARLSGADSRWERVEQATFLRNWLRWVSESDRDEQ"
     gene            24022..24699
                     /gene="nfi"
                     /locus_tag="P5316_01855"
     CDS             24022..24699
                     /gene="nfi"
                     /locus_tag="P5316_01855"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010045.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HRR83730.1"
                     /translation="MKLPPPIHRWSMTPRRAISLQQRLAGRIRIEPLRKMPRLVAGAD
                     VAFSADGRRCLAGVVVWDVEAGEVVEERLAWRPATFPYVPGLLSFREAPAVLAAVRKL
                     TLRPDVFMFDAQGLAHPRRMGLASHVGLLTDCPSVGCAKSRLCGEHGVPAAKAGSTVP
                     LLDHDETVGVVLRTRDGINPVYVSVGHRVTLQDAVSVVMSCVTCYRLPEPTRLAHILV
                     TRHRREK"
     gene            25707..29351
                     /locus_tag="P5316_01860"
     CDS             25707..29351
                     /locus_tag="P5316_01860"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HRR83731.1"
                     /translation="MSQCGRDWGCRSSVSRRDVLKIGAGTAVLLALGTSAQAEPGRIP
                     PQCKVPPGKEWFDQLLQPGKTITYTGKHLEKIIFPLGGIGTGTIWLHGSGRLVNWQIF
                     NNIQKNSLVDDSFFAVRVQQEGKPAVVRVLRQEPLGPFQGIKDIQFVGRYPFGRVSFT
                     EPELPVQIELEAFNPLIPLNEKDSAIPCAIFTLRAVNKSDKPVQVSFLGSLQNAVGHA
                     GVGASAGVSHAGYGGNLNVPIRDERMECVLMKAEPGRPAEFSPPVELIVDHQSLPAVD
                     TSPVRGLTLAGVGRQGRNPNIQTVYWLAVGDLKRVGGSLLKQVVEDVREYGAFLLLSG
                     SKNPLTNPLQPNLTAGVRRRETIFASFDDPSFGHWTAHGKAFQRPRGGTMPGQSPVTG
                     FLGSGLVNSYDPNDEPKGRLTSPVFVIQEKYISFLIGGGRHPNQCCVNLVIDGKVVRS
                     STGSNSEHLEREEWDVSEFADRQARIELVDERGDGWGHILVDDIRFSNLPTGAVTADE
                     AEAWNALLNDARPRATSRPAGVGRGKVMLMPMDVDATGSPVEKLAVRDKLLSMIASLT
                     DVTYRPQVGRQPDAPSFGTMCLATPEKDATILHDWSDRDVLYRLLSDSGRLADFKIPP
                     ATVGPTGAGQTLNGALCIERGVAPGLSAEADFIFTWHFPNQYYPQNSYQQSGRHGVAV
                     GNMYDNWYRDALDVARYVVANLAGLRKHTLAYRDAMFDTTLPQYFIDAVAANVSIIRS
                     PTCFWTKDGTFYGYEGCSPGGGGCCPMNCNHVWNYEQSLAKVWPALDRDMRRTELKFH
                     QNEAGGTNHRVAVPRDNPHKTAPPVADGQCGAVLKAYREHLQSPDRRFLDEYWEQIKK
                     AMDYAIKTWDKDGDGLMEQPQFNTYDRDIYGHNTFVTSLYLAALRAAEEMARLRGDKP
                     AADKYRLLFEKGRDAAAGLFDGEYYIQIADNINLGYGKGCWADQVVGQWWARVLNLGD
                     ILPDEQVRSALKAVFKHNWLWKIDGFEGTQRFLEFADGDDKGLLCGSWPKGGRPPDPI
                     LYRDECWTGVEYQVAAHKIYEGQVEEGLIIVRGARERYDGTKRSPWNEIECGDYYVRA
                     LSSWSLLLAAQGYAYDGPARRLAFNPRIGPEDHRSFFSTAEGWGRFEQKRGGNRQTDV
                     LAFDWGRCDLRELRFGLPPGAEKVTAKARLDDRDLFIETRLDDQDAVIILSAPVTAVA
                     GNTLTVEMSY"
     gene            complement(29576..33400)
                     /locus_tag="P5316_01865"
     CDS             complement(29576..33400)
                     /locus_tag="P5316_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83732.1"
                     /translation="MVMVVGVLAMLFIIGSTLLIVGRFERQTVQISMTARSMEAVSNG
                     ILEPVIIALRQDLLGSNNKAYLRDNWNNTLLGEDYGDYPGFIAANSPRNGDLLLSSLE
                     PYFDPGGPGTADDRWRFFAVSWPSDFPGEPNASRKNFNIFITDNPLEDADGDGIRDAA
                     INDILGLSDTFGGTYHMSLRVIPHNAMVWLNRMTHPTLLAQVVHPTDLAAVGGMDALR
                     TAIVGLGLGPTDENRLRRRFMLPPLLPEPDSGNYKNVASVLVQNLAITTGYADPAKPT
                     GWLNQPVNRWTMFRPSDKEQQDWERRIEPTYNGLTDPAYNAARDTYDRRHLITTESSD
                     DALRPQREEKRLKAFTFTLGVAPDTVTFNLENIISPVDASLVTPASADPNRPPLAYGV
                     MDYGPAGGSVDHVFNKDGLRTQFSLRDVLEPNGQASYRRATQLTAYFLAMIQHTTVPG
                     SNVPNPSDLRNPTPAELAEQLRTAMQLAVNTIDFADADQIPTYLEFDNGAGLAAKVVG
                     VEKQPFITEAYAKFIYKADGEGAAWDTGTDLSIYAVELYNPYDTPIQLGTVPDPSFRI
                     RVTDASGTGTVAVDVIDPTIAPALPQSIPPHQYYLVVNTKADESKSGDDFLTPVSPGK
                     IVENSALKLDDLHRFVRLYRSGTWKVDRDHPNGVLSTDPIEIDRLAPIDDPAWATAQD
                     SSKWLPANPQNLRNPRNKSDIFVWDCSLQRHKEPNPERPVHWHFTLSRQMVFPLKYQE
                     DKDEPTAASDPYVYLLDPSDKTRVWCDSTRPPQHNLLGTAPSTTDFANVVTVGATTRF
                     LTNQGYLAAEFAGQRLVSAVTASVQPEITVKPFPIAPFPVLVADRGVHPNTGGTMAFP
                     TTGTLLLVTRYAHLPMQGIMTEDKPLSVAAMDFVVRDFDALGNLIPLRSAAEQILQLD
                     NGHLPVFDTAQACLDDKNSRFGGLPWGQLAFEYFTALPMEELARDLGPLTPGAQRLMD
                     STDYLNHYGAVGGADNWGDWTKSRFLNYPVMMPVAQTLGPRVQGRIGIGSAPWWVLDG
                     LPVLPDFSPTDSVNVNPAWTYNLACLPVPEIEAERLDPYIHTADPKFMAGAWFVHRLI
                     DEKYSGTLPQPLSSTPNYYVDPPAEMPSISPRLAQYMVSYREQRPVGAVQAVYADRIG
                     FATTGEICNVLTKVQLGDLKNPKTGADIKGPSLPLDEWRRWADDNNERPFSYLGYLQL
                     VAPIVRMQDWMTVKNHVFTIYATIQSTGQPPVTVRTQVTVDRTRCLYNPSELPERITE
                     TPPISYYNMMDD"
     gene            34203..35123
                     /locus_tag="P5316_01870"
     CDS             34203..35123
                     /locus_tag="P5316_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR83733.1"
                     /translation="MVARFQKRDGVRGFTLIEVLVVVAIIALLIAILLPSLARARAHA
                     RNAADLSNQHQIGLAMNMFASRNNGKVPVGADPDDQDHWVSIVAREIGLVKRLTSDMC
                     VNHIRVDQIDLYQCAERTATGSAPFLGYVSNSLNPDGPSGTDWRPLDGVVNVASRYKT
                     PANVIYIMCAETEPKVWDGKNSLGYLSPLGARKNWESAVAAGLLAPGREAQLKSTLIN
                     SGGGLDCLDIWRGGQLPQYNCPDIRQPNPNRRVAFELHLNRFTNAMFYDGHAGPVPAE
                     KRSTWQENHRVWLQRCGLTPAGIAAALNIP"
     gene            35383..35982
                     /locus_tag="P5316_01875"
     CDS             35383..35982
                     /locus_tag="P5316_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRR83734.1"
                     /translation="MADSHSGECVFSGVGGESDALVIRADLPGRWYVAHTRARNEKIL
                     AEELSRFGIYNYLPLALHVTRSSRTNRISRSLIPIFPGYVFFHGSEEQRYLALRTNRV
                     ANVLDVVNQNQLLAELRQVHRLLQERGDFAVIPRIQKGQWGRIIAGPLVGLEGIVVRY
                     AGRYRVCMNVTILGQCVGVEVDYDLVEPIDAPAYLTTRP"
     gene            36983..38134
                     /gene="wecB"
                     /locus_tag="P5316_01880"
     CDS             36983..38134
                     /gene="wecB"
                     /locus_tag="P5316_01880"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRR83735.1"
                     /translation="MKIINICGARPNFMKIAPLMRAYRRYPAIEAMIVHTGQHYDDRL
                     SDLFFRELEIPKPDINLEVGSGSHAVQTAEIMKRFEPVALEQQPDWVVVVGDVNSTIA
                     CALVAKKLGIRVAHVEAGLRSFDRTMPEEINRLLTDAISDLLFVSEPSGIENLRREGV
                     DGDKVHFVGNVMIDTLRSNLEKADRSSTLSDLGLEPGRYNVVTLHRPSNVDNAESLGR
                     IADALEVIQQDLPTVFPMHPRTRNNLQKLGLAERFAGMKQLRIVEPAGYLDFLKLMGR
                     AAVVLTDSGGIQEETTILGVWCLTLRDNTERPATITNGTNRLVGNNPAKVIAAYKECR
                     KAPPSKTMVPEKWDGHAAERVAEVMVGGLEKSAGCAGCSCHGSGGAVRR"
     gene            38138..39916
                     /locus_tag="P5316_01885"
     CDS             38138..39916
                     /locus_tag="P5316_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83736.1"
                     /translation="MGRFGWAHRAAIGCVVAGMLAGQAAVASAEPYPLRWFYLASNFA
                     VAQNADRAIALIEEASRAGLNGVVLADAKFATLGKYNHDSPSNPYYRNIRRVLDVCRR
                     NGVEVVVYLPTISKAHGILAHDPNLAAGYEVRDAVFEVRSGTARLVQDPAVRVANGGF
                     EDAVNQRFRGWDRQDDPGSAVVADRGTARMGVMSARLQFGDARRLGHARLGQRLKVAP
                     RRCYRVSLWLQTGGLAPTESFNILIRGARGGQVRVLAYNRFSVKATQSWTQMETVFNS
                     LDYEEIDVYLGLFGVRSGRAWVDDVELTEVGLVNVLRRPGCPLTVRSWDGRATYVEGQ
                     DFEPVYDPLLGKAGDRAGIFDRFHPAPDIRFRKGVPDGTRLRVGFYHPLIIHANQIDC
                     CMSEQKVFDILEDTIRRLNRLLDSPKYWWLGYDELRTGGGCRLCKASGKTPGQLLAEH
                     VRRVSEMVRKERQGAEIVVWYDMFYPGSNAKDEYYLVEGSLTGSWEGPDPSVMVSKWS
                     VLGRDECMRFFADRGHRQILSADFDSVKDPAGFLQSWLESMDAVSGVQGIMYTTWTGR
                     YDYLSAFGRTLKSHSIPASASRPVPN"
BASE COUNT         7468 a        11603 c        12987 g         7958 t
ORIGIN      
        1 gcatcttgaa acaacttggt gtcttccggc gactctgtcg tgtggacgtc gttatagatc
       61 tcgacgggct tgctggcatt gaacgtcacg aaaagagtgg gccccgaggt gatcgtgtcc
      121 gcggagtctc gcacctgtgc gtggcgagag aggaagacct cgtagtggta gaagctctgt
      181 ccctcccact gacatcgctg catccagaca acggcttcct gactcttaag ttccctggca
      241 ccgacgtgca gcgagaaatc accgtggaac tgcaagacct gggtgccgtc gggctcgggg
      301 cgcagataca cgtagccggc gaagatttcg ggtttggcaa ggataatccc ggccggcagg
      361 agcgtgcttt ccgcctgggc atcgtcaatc gggagaagcg ggcgtggctg gccatgcagc
      421 ggaaacgccg tcaccagcca gatcaacagc acagtcccaa ccagtgaggc ggttcttgcc
      481 aatgtgtcgg gcctttccat ggtcaagcaa gccgcagcgt gggttcgctt gctgctatct
      541 tcggccatct ctgcgggcac gatgtcccca tgggcgggca ttataaaggt gagtgaggcg
      601 gcgtcaacgc ggccggacac tacataggcg caagtgttgc ggggacgcag tgcaatgagc
      661 ggttttgggc catcatcggc aaggccgaat tcccgcgcag tcgcctcctg gtcaggggcg
      721 gccggaagcg gttatttctc gacttggagg aagaacgtac ggtagccggc aatcgtcctg
      781 acccgcagcc ggacgcctct tgccaaggct tcctggctga gggcttgctt cagagctgcg
      841 accgttctca cctctttgcc ttgaacgctg atgatgaggc tgccggggcg cattccctga
      901 gcatgggctt ccccgatggg gtcaacctcg gtgatgacca acagattctt gaccttgtcg
      961 tattcctcgt cccaaccgta ttgcttggca agctcctcgt tgagtgcctc gacggtgatg
     1021 ccgattgcgg cgatctcggc gggaccagat ttgcggtctt ctttttcggt atcgccttct
     1081 tcggtgcgtc ggccgcccca ggtgaagaag tcccgtggct gcttctcgat cgtgaccggg
     1141 atggcgattt ccttgccgtc gcgccagacc ttcaggtcga ccttggtgcc cggcgaggtt
     1201 ttggtgacca tcatggtgag ctgcgtggct tccttggtgg gcttgccgtc gtatgagagg
     1261 atcacgtcgt ccattttcag gcccgccttg gccgcggggg tgtcttcaaa gacgtcctcg
     1321 acaagcacgc cgtcgtcatg atccaagccg aaggtcttgc cgaatcccgg tccgtaggcg
     1381 tcaaggctga cgatgcgcac ccccagatag cctcgaacga tttccttgcc ttccttgagg
     1441 gatggcagga tttctttgat cgtcttgctg gggatggcga agccgactcc catgtatccg
     1501 cgggtgagac tgttggttgc gatggcggta ttgatgccga tcatctcgcc gcgcatgttg
     1561 accagcgggc cgccgctgtt gccgggattg atggccgcat cagtctggat gaggtcttcg
     1621 tagggaatct cgagcaggtg cgagcggccc ttggcggaga tgatgccctg ggtgacggtc
     1681 tgatcgaggc caaaggggga gccgatcgcc agaacccagt cgccgacttc cattgcatcg
     1741 ctgtccccaa tggtgatggc gtggagtttg tccgcattga tgcgcaggag ggcgaggtca
     1801 gtcttgtcat cacggccgac cacgtcggct tttaccttcc ggccgtcggg caaccggacg
     1861 tcgattcgcc cctggccgtc ttcaacatca gagacgacat ggttgttggt aatgatgtat
     1921 ccggcctccg cgtcgatgat cacgccgctg ccggtgccct cgcgcgggcg ctggggcagg
     1981 agacgcggat cctggaactc ctcgaaaaac tccctgaaag gctcggggat ctgcttgggg
     2041 tcaatctggg gtctgcggcc tcgacgttcc ggcggcttgg cagtcgtggt gatgtgcacc
     2101 accgtcggct tcacctgctt ggccacgagc ctgaatgcct gcgaaatcgc ctcgacctta
     2161 gccaggtgct ccttgtcggc ctcgcgcacg cccttctcga cggcataggc gacgctcccg
     2221 atgatctgct cgtggaagta cattccgacc agtacgagga cgaccatcac gagcaggaaa
     2281 tgaagccact tgttgttgga ttcggccatt gatatatgcc tcctcggtct gcaccctcag
     2341 atggtattct gcaagggcat caaaccgtaa cacccggaag gaattgatgc ctccggcggc
     2401 cctgaagcca tcgtagcgtc gccctaaggt ggatcgcttt cttgcgagcc gcaacccaag
     2461 cacactgcga tcccgaaaca tgcgcgcagc tcgcacacat gtccgaccgc aaggttctgt
     2521 atacttagac acactgtccg gcgcacggtg ttagttatcg gccggtttaa ttcctgaatg
     2581 tactgtaaca catggccggg caagctacta tgggactcag tgctgcctga cggatggtcg
     2641 tctcaggcga gctgcaagcg gtaccagcag ggttagcaag gacagaagtg tcagtccaat
     2701 gcctgcgctc agtcccacag ggcggtatcg ccagataatc cgatgacgtc cgggcgggac
     2761 ggcaacgcct tgcaccaggg cgttgacccg gtgtggccga gacaccagtc catcaatctc
     2821 gacggtccag ccgtcgtcgt aagtctcgtt gaagacaaga aaggcagcga ccgtgctgct
     2881 tgtgtcaacc agcagttctt cggctgaggg tcgccgccat tggagcgatg cggccgatcc
     2941 tggcgcctct gcaaacacgg gttgtccgag gtcctcgact accacatggg ttgggtcttc
     3001 aagctggctg ggtcgggcct gcaggagctt gacggcttgc agcaggtcgg tcgccggcgt
     3061 cacgcgcttt gcccagaata gcagaggact ggcatttctc aactcataga gcgagatgtc
     3121 acccggcaga tcggtgacgt ggttccaggg tgatcgggct tcagtcgccg caggcgcggt
     3181 tgaaatcgtt tgcgggggtg ccgccgcgcg tccgaatgcg gcatggctga cggtcacagg
     3241 ttgaccatac tccgctttga ccctgactct gatcgggccg taggcttggt cgcaccgaaa
     3301 ctggaaacgc atacggcgag gaccaacggc aaggtcctcg ggcgtcaaca ttcggatttc
     3361 ttcgatctgc tcgacgtttt gggtttccag cctgacgaac cagcggttac tctctccggg
     3421 ggtgggagtt gcgtcgattt caagctcata aattccggcc gacgggatgt tgacgggcca
     3481 gagcaggtcg tgacctgcgc ggaccggaac cggtagttcg gttccgtcaa tgagggaaag
     3541 gttgggcgct ggggcgtccg gccacaaggc ggcctcgacg agtgctcgct cctgtctcga
     3601 tcgtaccgca atgaaccgca cccccagcga tttgagaagc ctggggtcgc ggagcagaga
     3661 aagcatttcc tggctcgatc cccagggcat gaaccgcaga agcagccggc tggacagcgg
     3721 ccagagcggc ccgtagccgt ggaccgtcga aatgtcgcgc tgtacgtttg tctggggcca
     3781 gagcatctcg atggggcgat tgtagtcggc cgaaaaccgc ggaacgagaa gccgatggcc
     3841 gtcgtccggt tttgttgctg cgatggcgcg ggccagttcc ggcgggtttt gcaggtccga
     3901 ccgctggtat gtccggacat ccacatcgat gaaggcggcg accgaggccg cgtcgaccat
     3961 catgaccaga aacaagagca cgagttggtg cgtgcggcca cgatcgaccc atcgtagcaa
     4021 gagggccccg gttgcggcgg caagcaggat gggccaccag atagccgggt tgccggggcg
     4081 aatggccgcc cgaagtccgc tgaagaagac gtctgcgttc cagcgtcccg cgaaggccga
     4141 ttccagcgag ttcacctgcc atcgagccgt caccatcagc agcacacaaa ttgcgaaggt
     4201 gatgggcagg atgcgtttca ccacatatcg gattccacct gtcgctttat cggcggctgg
     4261 ttgtgatgac aatacggcgg agatgaccgc cgaagcgaga atcggcatag ccagcgacca
     4321 gccgaggatc catcgtgccg gaactcgaag gctgcggtaa agcgggacgc ggaacagcaa
     4381 cttggccagg ggggtgagct cgccgagagc cagcagcaga accacaaacg aggcgatcca
     4441 ccagaagagg atttctcgat tccatgggcg cgaggaggct cgctgcagct cggtaccctg
     4501 agctcggagc gaggccaggc gaaccgacgg tcgttgatgg ccccgcagca gccaataaga
     4561 cgccagcgcc agaaccagaa tcaggatggt cccgtacgcc gactgctcgc aaaaatggga
     4621 gatgccccac cacggctggt gccagaggtt gggagtcctg tttccgaaaa gcatcgggaa
     4681 gagcagcagc agggctgaca atggcgtcca cgagttctcg acgaatgttg cgtacgaggg
     4741 ggtggcccgg ccggtggccg acaggtgcag cagtgttggc aggaactgaa tggcggacac
     4801 gagaatgcct gcggcgacac cgagcgggaa ttcccacatc agccgaggac ggcgcgggcg
     4861 gaggaccacc agcgcgtacc cgctcagcaa ggccaggcca atgatagtga cctgaacatg
     4921 ttgcaccagc atttgcgcgc ccagggcaag cgctgcacga gcgaagtgct tgtagcggcc
     4981 atcggtcgag aatctctgcc attcgtagaa gatgagcggg agccaggcgg cggcgtgatg
     5041 gatcgtcagg tgagcgcgat gcgcgacgag gtatccggtg atctcgaaag caatcgctcc
     5101 gaacaaggcc gcctgccaat accgccccag cgatcgcaga aaacgataca ttcccattcc
     5161 tgcaagggca aaatgcagga tgatagtcac cggataggcg accagcggcg gcaagacggc
     5221 gaagagccag gtaggcggat accacaggcc gctttgcggg tctgccgcga tcgagattcc
     5281 catgcggctg aacgggttcc acagaggcca gtctcccgcc gtgatccgct caccgatgta
     5341 ctggcggacg ggcaggtagt aaatgaggtc gtcttcaaga gccgaaacgc cacccaacct
     5401 ccacgcaccg gcgagaataa cggccggcga gagaatgatc accgagctga ccaatagtcg
     5461 cctttgccac tcccgaaggg gctgggggct gttggttggt aattcgagcg atgccatcgc
     5521 attcgatcat gctgtgcgcg ttgaccgtcg tcaagaagga caacttgtgt cgtggcttgc
     5581 atcaggcgag aatgactttt gaacgagtgc tccaggctcg atgtcaaagg cagaggggct
     5641 ggatggcagg tgtctgccgt catcgtgacg gtcaagcgcc tgtaggctca gccttgttac
     5701 ggagagcgtc ttcatggact cagacgacga gacggtcaag ggcgtgattc tgacggctcg
     5761 aatcatcagc ggcgccatga tcgcgggggt ggccacgttt ggagtggtcg tgctgctttt
     5821 gggaaacgtt gtggcttctc agccctcgct cggcgggctc tttatgggca ttctcggcct
     5881 gattgctccg gcctttctgg ttgcttatgt ggtactcagg ggtgtgatgg tcagagctgc
     5941 caggtcacag tgcgagcaaa gcggcggggt gggaatgtcg ctcgaagcgc tggcacgcgc
     6001 gtacgccccg ctggtcatca ttcgggcggc catggccgag ggagttggct tggccggcct
     6061 ggttttcctg ctgatcaccg ggcagaagct tctgtgggtt gctccggttg tcgcgatcat
     6121 cattcttgtt gccgcgctgc cgagccgaag tcggttcgac tcgttcgtgc aggaggtgac
     6181 cgggacgaac ccctacctcg gctgatgcct tgtcacgcgc ggaacaatgg ctccgtgcgt
     6241 ccccggagtc catccgccag gcactccctg agaaagacct gtccaaccgg gttgtcatct
     6301 ctggctcagc ctttgccaca accacatgcc gagtgtaagg atgaccgaca ggacgatgca
     6361 ggagacgatg gggaaataga agtggacgtt ttccgattca tagtggatgt cgccgggcag
     6421 gccgcggaag ccaagtcggc cgagcgccca gatggccgcg ccgatgacca ccatcacgat
     6481 gcccaaaacg atgatcagtt tgcccatcgg gatcatgaga taacctcgcg cacgtccatt
     6541 atcgttgtct ggtgcggaac ctgccagagg cgccgtcttt gggtcgcttg cgccttccgg
     6601 gccccgcggc ggcagtcata tcgagctttc tggaagcgta aaccacgcga cggtatgatc
     6661 ttgccgtgcg aaacggcgag actcccgaca gcatgagggc tttgacgtac tcggttcgcc
     6721 cggtgcaatg gaccgtgtgc aaggtgatgg gctggctgtg gccgtcggtg ttctggtcgc
     6781 ggctgtcggg tttgcggctg agtgaggtgc cggtgccgac gctgcccggt ccgcgctggg
     6841 tgcggctgaa gacgatcttg ggcggaatct gcggaacgga tttttcgggc atcatgcagc
     6901 gggggcaccc ggcgtcgatc ctgcaggcgt tttcgagctt tccggccttg ctgggccatg
     6961 agaatgttgc tctcgtggat gcggtcggcc cggcggtgag cgactggaaa cccggcgacc
     7021 gggtggtcgt cgagccgaca ctctcgtgcg tgcctcgcgg catcgatccg gtgtgccgaa
     7081 attgtgcggc cgggcgattc actttgtgtg aaaacttccg ggccggtccg ctgcctgtgg
     7141 gttcgatgat tggttggaac tgtttcacgg gcggcagttg gggaccgttt tttgtcgccc
     7201 acgagtcgca actccaccgg gtaccggatg agatcgacga ccggattgcg gtgttgatcg
     7261 acccgatggc aggagccctg cacgcggtat tgcgacgcaa gccatgggaa catgagacga
     7321 ctctgatcgt tggtgacggc ctgctggcga tgggcgtagc ggcctgcatt cgagctctcg
     7381 gcagccgctg tcgccttctt gcgatcggcc tttgggaaac actgcgggaa acgatgcagc
     7441 ggttcggcgt cgatgagttt atcgcggtcg gcgggcgtaa tacgcaggcc gcgcggtacg
     7501 ggaaggtcgc cgctcgcgtg gggggcaagt tggtggcgac caagttcggt caccaggcgt
     7561 tcatcggcgg tgtcgacctg gtctatgact gtgtgggtac ggggcagacg ctgacggatt
     7621 cgatgaaata tgcgcgctcc ggcgggacgg tcgtagaagt ggggacttcg cagatctcgc
     7681 tggtggacac ggcgccgttg tggtttgacg agctgaccct catgggcacg aacggtcggg
     7741 cgatcgagca gtacgatggt cggacgatgc atacctacga gatcgtgatc gagttgatcc
     7801 gcgagcgaaa gctcgacctg tccgggttgc tgacgcatcg tttccggatc gaccagtatc
     7861 ggcaagcgat ggctgcgctg gccgatcgag cgagaagcgg ggcaatcaag gtggcgttcg
     7921 agcatccatg agagcgagcg attcgttttg tgtccggaga ctgcatggat ctggccgcgc
     7981 gtgagtaccg acgcatcctg atcatcaagc cgagttcatt cggcgatgtg atccatgcca
     8041 tgccggtgtt gcacggcctg cgaacgcgat ttccgcgcgc ccggatcagt tggttggtgg
     8101 cccatagctg cgccgggttg ttggaaggtc accccgacct ggatgaggtc attcgctttg
     8161 accgcaagcg gtacggtcgg attggcaagg gactgacgcc gacgattgag tttgttcgct
     8221 ttctgggcga cctgcaccgt cggtccttcg acctggttct cgacctgcaa ggtctgtttc
     8281 gcagcgcctt cttgactctt gcggcgggtg cgcaagtgac gctcgggttc gccaacgccc
     8341 gggagtttgc accgcttttc tatggtcgcc gaatcacggt gccggaccct gagatgcacg
     8401 cggtcgaccg gaattacctt ttttcccgtg cactcggttt tgctgatctt ccagtgtcat
     8461 tcaacatgcc tgttcgctcc gaggcagcgg cggccgtggc aaccgtcctg gcggatcacg
     8521 gcatcgcgcg gggtcatccc tacgctctga tggggcccgg aacccgctgg gagaccaagc
     8581 gctggccggc tgaagcgttt gccgaggtgg ccaggcgggt ccgcgcggaa ttgggcctgc
     8641 cggtcgtctt gatcggcatg gaggatgaag ccggcgtggc ttggcaggtc gaacaggccg
     8701 ccggagaagg gattgtgaac ctggccgggc ggacaaagct gcccgaattg atcgccttgg
     8761 ttgccggggc ggcgctctgc gtcatgcatg attcgggacc gatgcacctg gctacggctc
     8821 tggaccgtcc gatggttgcc atctacgggc cgaccagtcc gcggcgcacc ggtccgtacc
     8881 gtcgtccgga ggcgatcgtc cggttggacc tgccgtgctc gccctgctac atcaaacgcg
     8941 tggcggactg cccctacgat caccgctgca tgcggaagtt gcctccggat actctgatgg
     9001 acagactccg gttatcatat ccgccgggag tgaacgatag gtgagaagaa tgtccgtcac
     9061 ggtgctgccg gttttgctgc tggctttgac ggccgagggc caggtttcgc gcgcaacgcc
     9121 ggtaacgccg gtgctggtca cctcgtcggg cgagtatgac gctgaacgcc aacttgtgac
     9181 tctgggggtc gggcgcgacg agggggtcgg tgtcggcgat cacttctggc ttttccatca
     9241 ggagaatatc acggggaccg gaaccgtcta cctcgtgtcg gggacgcaat ccgtaggcag
     9301 gctggctttc acgagcagga acgtgggcgc caagcagtcg gccgtggttc ttgtcggggc
     9361 ctctttaacg gccttacggc cgcaggtcag ctctgaggtg ccgatccgct cacgggtggt
     9421 tcgtgtgccg ccggcgaggc gcacggcttg gcttgacttc ggccggcggt cgggccttca
     9481 ggagggcgat cccgtggtca tccggcgatt caaccttccg attgcccgcg ggcagcttga
     9541 gatcgtcgag gatgaggttt cgctggcggc actgcatccg ctcgtcggca acgcattgcc
     9601 cgagccgggc gacgatgccg agatctggcc gccgccgggc gtggatctgc aggtgcgact
     9661 caagagcacg attctcgatg tcgagcggtc ctcagaggga ccggtgatca cgtttgttgg
     9721 aacggccgcc gatggcatcg tggagaacag gctggttgac atcttccgca aggagcagtt
     9781 cctcggcgtt gcggcggttc gggaggttta tgagccgctg agccgggctt tggtcatgga
     9841 gccggccacg cgcggcatgc ctgaggtggg cgacacggcg ttggtgcgtc tgcggcccgg
     9901 gcaacctccc aagccgctga ccgccgtcgt gttcaaggtc gcgggcgact attgccttat
     9961 cgccgcgggc gaaagcgacg gcgttcaaca gaatgagaag tttgtcgttc gcggcccgat
    10021 ccccggcggc atgggcggca tgcgggagat tgccgagctg accatcgagg cggtgaaagt
    10081 ggattactca ggggcccgca tcaggccgct cgctgctgac ggcgtgaccg tgaaggcctg
    10141 ggatatggct gagcgccgca cgatccacga gagatcggag tggatctatg ccgggcgagt
    10201 cgatttgact cataccgatt cgcgaacgct cgttgccggc gcggaacccg atgtggctct
    10261 cacgggaggt caggtcgttc gctggacgcc gccgcaggca accggcggtc agccgggcgc
    10321 cgcagtggtg atctatgtgg gcagggacaa aacgtttctt tttgttccgc cgtgctgggg
    10381 caatctggcg gaggcgcccg gggcgcgcgt tgagatacct gcgtccgagc tgtcgccgtc
    10441 tgcgtcggac gaagtcagtt cgacccggcc cgcgatccag acgacccgtc cggagtagcc
    10501 gtttgcccca tgggtgcggt ggggcggcga tcttgcttac cgtgagtcat tcctggaagg
    10561 cagcgcgaga aaggacggtg tatgtggggc cgccgcgatc gaggaccgat tgaaacacgg
    10621 tcagttcctt cacggtaaag cggccgccgt cgaaatgcag ttccgattcg agggctggcc
    10681 ggatcttgtg cgagcccctc tggtctcgga ctcgcccgat ggtcagatgc ggatgaaacg
    10741 gctggttctc gggcgggaag cccatctcgg cgaatgcccg ttcacaggcc ttgtgacagt
    10801 cgatcagcgg ctgcggcaga cttgccaagc cggcccagac gatccgcgcc gggcctcgcg
    10861 gcgggaagca gccggtgccg gacacctgga tgtcaaaagc cggatggtct cgggcaatgg
    10921 catccgctga ttggcaaacc tgtcccacgg ctgcatcggg aacctcaccg aggaacttga
    10981 cggtcaggtg aatctgatcg accttcgtcc accgcacctc gcgatccagc gcacagaggc
    11041 gcttttgcag ggcggccaga cgctctcgtg tttcacgcgg caactcgatc gccaggaagc
    11101 atcgcatgcc ttcatactaa cggcggcgcc gaacaccgca accgtaggcg gcatgatcgg
    11161 gccggtggca tccggtccgc acgcgaatcg tcgcaagtcg tcatctttga ccgtcagcgg
    11221 agtcgggggt aacatacgac gcgaatcgga aacgaaacgg tcggagagta cccggaggac
    11281 agtcaaatga acgagagtct tgatcgtcgt ggtttcttgc agggcggcgc cttggctgct
    11341 gccgcgatgg cggtgggggg cgatgtaggc atcacggcca ccaaggccgc ggcaacggag
    11401 gacaagatga acacgaaggc cgtgctgcgg ctgtcgagcg gcgaaggcat cattcccggt
    11461 aattctttga ccgaaaagct ggacaaaatg gaaaaatggg gcttcgaggg cctggaggtc
    11521 ggcggaggat atctccggag taacgtcaaa cagctcaagg aggcactcaa gggccgggcg
    11581 gtcagaatga gcgccatctg cgcgggcttc gatggctggc tgatcgccga cgaccccgcg
    11641 attcgcgaca aggccatgac cacgatgaag gaactgctca ccatggcagg cgaggtcgaa
    11701 tccaccggct tgatcatcgt accggcgttc aacaatcaga agtcgcttcc gcatcagcag
    11761 gcccgagaac tgctcaccgg tttcaagcgt tgggacgcgc cggccgcgga acgtaaggcc
    11821 ggcttgctcc aggaactggg tgatcacgcc tccaaggccg ggacgcggat cctgctcgag
    11881 ccgctcaacc gccacgagtg ctacttcctc cgaacactgg ccgacgcggc ctcgatctgc
    11941 aaggacgtcg agaaccccgg cattgcgatg atgggcgatt tctggcacat gacatgggaa
    12001 gagaccagcg attgcggggc cttcctgagt gctggcaagt accttcacca cgtacacatc
    12061 gccagcagac gagaacggaa gacgcctggc gaggatggcg ccgcggacaa ctacgtggac
    12121 ggcttcagag gactgaaaat gatcggatac caggatttcg tcagcctcga atgcggtacc
    12181 aagggtgaca aggagaaggt cctcccggcc tgcgttgcgc tgctacgcga gcagtgggag
    12241 gcggcatgat tgccgctctg ggcacagcct gagggtgtta ttccaatgac cgatatcccc
    12301 ccttgggccg gctcgcagtg acggcgctca agtgacctct tgcaggattt ccggcaaggt
    12361 gaagacatgg tgatcgagtc cggccggcaa ggacccgccc gtgacatccc gggcggacaa
    12421 acgggtgagc cggttgatta gaacgagctt ggtcgcgagg ttgtgactgg accacttgtc
    12481 gaagaagtgt tcgagaagct gcaagtatcg gggcgacacc gtgtcgtcgg cttcaaggct
    12541 ccgcagtgac tccgtcacgt aggctttctg tgccggggtc agcgtcctca acgatcgatc
    12601 gagagcccag gcctgcacct ccggcaagcc ggcgatgatt cgctcggtgt tcatgatttc
    12661 ctgttcgagg gtgtccacgg gttttctccc ccgccggttg ccgcgagcct tcctaatcgc
    12721 cgcggccaac cgtgcagttc ttcaggcggc tctcaaacga aacatggctg atcgtcgatc
    12781 aaagctgttg tcatgatatg ctcatcgcag cgagccgtcc tgataccgcc tgaggacaag
    12841 gctcgcggtt cccacagata tctcttcggt tgaataccgc gacctctgaa tagcttcttc
    12901 cgaaattcag ttccaaaaga atgaaatttt gaattcccat gggttcggcg gtgcgtgttc
    12961 aagaggcagc cgttcatgtc gggagacttg cgacgcgaga tgcttttgtc gacaattcgc
    13021 gattatcgga caatccggct tcggcagccg attccgtctc ccgaagtctt ttgctcagag
    13081 ggccatatga aaagatcatt gttgcaggtt ttgtccatga tgatatgggc gtcgcaggtc
    13141 gctgttgccc aggaagaccg ctacgccgtc aacaggggtt tgccgggtga cgccatgata
    13201 cagacttatc tggaacatgc ggcggccagg atctccggac ggtttcttga agggaccgat
    13261 tcgctcgaga actggaaggc catgcggccg cggttgcgac aggaatacct gtatatgttg
    13321 ggcctgtggc cgctgccgga acgtacgcct cttaacccgg tcatccgcaa gaccatcgat
    13381 ggccagggat ttgcggtaga tcttatgcac ttccagagcc ggccgggctt gtatgtcacg
    13441 gcgaatctgt atcgtcccgc aatcgtggag cacggtcgcc ggctccccgc agttttgtac
    13501 gtttgtggcc attcatcgag ggggcgggac ggaaataaga ccgcctatca atcccacgga
    13561 atctggtacg ccagacacgg ctatgtttgt ctgatcgttg acacggttca gctcggagag
    13621 attgccggca tccaccatgg gacgtatcgc gaggggcggt ggtggtggca ctcgcggggc
    13681 tatacgccgg cgggcgtgga atgctggaat ggcatgcggg cgatcgatta tctggtgagt
    13741 cgggacgacg ttgacccggc tcggatcgcc gtgaccggaa tcagcggggg aggagcgtct
    13801 accttctgga tcgccgccgc ggacgaacgc gtggcggttg ccgtgcccgt cagcggcatg
    13861 gccgatttgc cctgctatgt ggggcagcgg ctcatcaacg ggcactgcga ttgcatgttc
    13921 ttgcacaatg ccttccagtg gccgtggacg tacatcgcgg gattgatcgc cccccgtcct
    13981 ctgctcttcg taaacagcga ccaggatccg atctttccga tgagcgccaa cgagcgcatc
    14041 atcaatcggc tggaacgtct gtacagcctg tttggaactt ccgatcgcgt ggacgcggtg
    14101 gtgagcatcg gcggccacga ataccgcaag gacatccgcc aagcatgcta tcgattcatc
    14161 aacacctggc tgaaaggcga ccctggcctc gtcgaggaca gcgaggtgga tctggtaagt
    14221 cgcgaggagg atccgagcca ttatccgatc ccggcgtcga acctccgtgt ttttcctgac
    14281 gattctgata ttccggcgga ccagatcaac accgttgtgg atcgggtctt cgtaccaatg
    14341 gccgttcccg aagtgccgtc ggcgcatcgc ctgaacgctt ggcgggaaca attggtccga
    14401 gagttgcgcc gagtatcctt cagcggcttt cccgagcgca ttccgccggc cnnnnnnnnn
    14461 ncgagacggg caggggcgtt tgcggctggc caccgagcag ggcatcgaat gctgtgcgat
    14521 gccggtgggc gttgtccccg agatggtcag gcaggtgctt ctcgttgttg ccggccggga
    14581 ggaggaggtc gtggtccccg ccgtgattga gacgcttaag caggacggcg aggttgtttt
    14641 ccgatgtgaa ccgcgaggcg tgggcgggac acggtggacg cgaaagaacc cgcccaacta
    14701 cgtcgagcgc gcccatgttc tgctggggcg taccgttgac gccggtcggg tatgggacgt
    14761 tgttgcgacg gcccgtttcc tggcaatgga tcggaaggtt ccggtacggg tcggcggcgt
    14821 cggggcgggc ggaatcctcg cggcctatgc tgcgttgttg gagcccgaaa tcggcggcgc
    14881 ggccgtgtca ggcgtgccgg caagccacat ggatgagggt gctccacaat tgctcaacgt
    14941 gctgcgggtt tgtgacatcg ctgatgtcct gggtatggtg gctcccaagc cgttgcggct
    15001 gcgggccgtt gccccgtccg tctcggaaaa ggtgaaagcg atctacgctg ccgccggggc
    15061 ctccgacaga ctcttgatcg aatgatttcg tgttcctgag gcggtgggtc agaatctcgg
    15121 cgacggcgtc acagagcatc ctactcgcaa ttcagatcag ccggcacgtc agccccgctc
    15181 aagcatccca tgaacacggc cacgtcgtcg tggtctacgt cgccgtcttt gtcgagatcg
    15241 gccttggcgc agtcagggtt ggtctgcttg atgccgaagc cgctcatgca agcctgaaga
    15301 aggccgaaat cagcctggtc gacgtcggtg tcggtgtcga ggtccgctgc gacgccgccg
    15361 atgatgactt ggaccgaaat ggcctggggg ctgttccaag cattctcggc gccgacgaac
    15421 acgatggctc cgtaagaccc gctttgccag ccctggatca ggtcgtttct gtatgtcagc
    15481 gtgatggtgt cggcttcacc catggaggag ccggcggagg ggcttatcgt gacccagccc
    15541 gcggtgcagc cgatggtgta gtccatcggc gcgatcatgc tggcgttggt tactgtgaag
    15601 gtgtcgtcgg ggaccgcacg tccggggtat ataacgtgca cgatttcctg cgtgtccaac
    15661 acgattgccg ctgcggcaaa cgtaaaggtc atgaccgagc ccgaattgct gatctgagac
    15721 aggccgagac cggaggcgtt accgtcccac cacttggtgc tgggcgtggt atgggagccg
    15781 cacgcggtat aggtcgggct tttccagagg tcggttccgt ctccctggtt tgcgccgtat
    15841 tcgaggtccc atcgcccgtc ggcttgcacg agcgtcacct cgttgttgcc cgggtcaccg
    15901 gtggtgttgg tgccgtaggt gtcgatgtgc cagatggcca gaccggcgtc cggcagagct
    15961 gtgtcgcggc ccgtttgctg acggttttcg atcaaatagt actcgtttgg cttcgtgggg
    16021 tgggagtatt tgtacacctg gttctgatcg gagctgattg acaagcctac ctggtggctg
    16081 accagcaacg tcgtctgagt ccatccggcg atgtacttga ggtaggcgca gggttgcacg
    16141 ggattggtgc ggcttgccat atcgcacatc aggcagaact tgccgatccc ggaggaactg
    16201 ccggcgtagt cgtacaggtc cggccagttg cacacgaggt ggccgttctc gtggcagaag
    16261 gtatccaggt agagggcgcc gtccatgtcc gtgatctgat accgggatgc ggtgaccccg
    16321 tcggccgaga atgaaatggg cgcgcagtga ggccacaggc cgtaggccca tcccgccgaa
    16381 gtcgtgcccg cgtagaggca attgatggcg tcaatctggc cgtcgcggtc ggcgtcgtac
    16441 atgctaaagt ccagacccgc cgcgtcgagc gcgttaagcg cctcgatcac caactcctgc
    16501 gctcgcgtcg ggcaacggta attcggatta tcatagtagc tcttcggctg agaggcccgg
    16561 tagtactgtc cgggaacaaa gtttgtgtac gtcagatgcc ccttggatac gtggtaaaag
    16621 tagtcgcgaa ccgagccgtt gttgccgaag ccgttgtacc cgatctgatt gcagaagttc
    16681 caaacggttt ggggagggat cgtgccgggg tcgtcactga aatcgaccag aagacaaatc
    16741 ccctcaaccc tgccgctggg cgtggtggca ggaacgggtt cacccgtgcc ggcgacctgc
    16801 ggcgtgccgg cgcttgccgg ggcggaaaac gcagctcgcg ccgctgagac ccggttgcgg
    16861 atcgctgcgg ctgagggtcg caaatgacgc ttcagagcga gggattgggg aatggggtgg
    16921 ccgacgacaa cgccactcga tgcaaggtct tctttgccgt cggcaggttt ggcatagcaa
    16981 atcgcaccgc ttgtcgggtc gcgcacaagt gcgaagccgt cgagggactc cacaatctgg
    17041 tgatattcgt caccccagat tcggacttcc accaacgttc catccggctg acgaagcttt
    17101 gttcgtttgc caaatacggg agcagcccat gctggaacgc acatcccgca cgcggtgatc
    17161 gtggcgatca accgcaagat tccatggcct gccagactgg tccaaatccg ctgcaacgct
    17221 caccaattcc acgagacagg atagcgcaga cccgatcggc gcccgaggat tggccgccca
    17281 gagccgacgg tcgtgacagg cgacccccca agcccgctcg aaaccgcttg gcaggcagcc
    17341 gtcgcctacc aatgcgcatt ttcccctgcc gcagaacgaa cgcacccaca cgcccgtcac
    17401 gggctgcgtt gcacgtcgat ctcggggaac cttgcggggc cgactctcgg cctgtgtttc
    17461 ctcgctcgcc atattgtgta cccgcagccc cgttgcggtc aagcaagaaa tcggattgtg
    17521 gctcgatatc ctgcgccgct caaaaggttt atgcaaagcc cgtatttctc tcgaagggga
    17581 ggcgaacggg cctgcaaaaa ggagatcaga acaccgcaac ccaaaacaag agcccggggt
    17641 gacatccggg ctcgtgtggg agacggttgc tcccgagatt tggtggtata ggaaatgtaa
    17701 cccaagttcc tgggcgaggc aagtcattta tcggggtaat tcctgcctac ttgggtgggg
    17761 ttggggtgtt gggatacgat cagatggctg gcgccaccgg tctcactgcc taagagacgc
    17821 ttggccgtca gtcagaggat gggcggcgct tcccatgttg aagtccaaac cccctgtctg
    17881 ccgtgcagca ccccagccaa ccgtgctcct gactgaccgc ctctcgatca atcctatctt
    17941 cgtaagttct gcctatgaat ggccttacat tgggcggatt tgaccgagcc gaacctccgc
    18001 cagtactggt atccgcgacg gcttccaggc ttcatcgggc ggtgactcca cggcaggtga
    18061 tcgggttgga agtatgggtg gttccaggct atttcgtccg ccgctgccct tttgtccgtt
    18121 cggtcccacg ggactgttct gccccgttgt aggcgtccga taaagcaggg tgcgccattg
    18181 atcttggctt ggacgaagat cctgccctga gacaggcagc ggtagcagcc ggcatcacag
    18241 gcaaggcggc aggcgatgcg gaggcaacgg tatggactgc gttctggttg gtgtctatcc
    18301 cccgaatctc agcccccgcg cgttgcggag tcttcaccga ctcctcaagc gggacaaccg
    18361 gcgggcgaag aagttttgcc gatggctcga tggcgtgatc gtgcgcgaaa tgcgacgccg
    18421 agtccgagag cggagcggac agtatgtcga ggctgaattg cccgaaattg acttctgcaa
    18481 ctggtccgac gccgaggttg gtgccggctt agtcgtagcg gcggtactgg tcgatgccgt
    18541 ccgggatcgt ggcatggccg ccttcatgcg gcgactggtc ttcctctttt cgatcgaggc
    18601 gagagaacgt ctcggcggtt gagtggaggc gattgtccct gctcagcggc gagaatgaag
    18661 gcaacacaat ccggccgggt ctggttgctc ctcacagcgc cggctctaag acacgcttgc
    18721 gtgagcgggc agtctcctca acatcaggtt cagctttgtc gcaggcggtg atccagcctg
    18781 attcaatcat gctccggctc tcttgcctct ggcggtgcgc gacgagaaaa cgggccgacc
    18841 cggtcgactc tgacgccgtg cacaatggga atcgtcgtta tctcatccgg ctcaatgggg
    18901 gcttgaattg tgcacgttga gcactgcatc tcaaagaggc accaggtaag accccgaagc
    18961 ctccgctgcg cacttggggc gccagtaagt ccgctgaccg ctggaacccg ccccggtcaa
    19021 gctctatctc ggacctgctg acacgctggc ttatagggtg tgactcttcc tgttggcgat
    19081 aatagcggtc tttgggcgac aagactggcg gctcgagggc gatcccaaaa acacccgaaa
    19141 aacgtgcgaa aatcggcttg acttctttcc cccgcggttt ttttgttgta tagttattat
    19201 gacagttgac tcccccgcac tggctctccc caggtagcgc gaagggcgga aggcgtggtc
    19261 tttcggacct ttgcgagcag cctgaaagtg aataccggaa aacgattgga ccgtggatcg
    19321 tttgtgacgg cttgcgcgat ccgggcgagg taatcggacg tatcaagggt tggatgccct
    19381 tgaaggcgcg cggctggtgg tcagtttcca cgatcagagg gagtcgggtc atggcaggga
    19441 caaagacagt cacaacccgg gcagttgaag cggggccggc tgccgtgaga ttcgtgctgc
    19501 catccgaggc cgacatggct cgcctgagcg cgacggatca ggcggtcctc aggatgctgc
    19561 acgagaacgc cgccgacttt gtcggctaca tggatcatcc ccttttcgcg aagtccaacg
    19621 tggagagaac gttgtttggg aagaaacaga ccctgctgcc cgggcagacg cggttcaagg
    19681 acgcgagccc ccaggatctg atgaagcgag ctgcggatgg tctgaccctc agctccgctc
    19741 aggagattct gctcttccag cgattcaact attcgcgggc cagggtggcc cgtttgatgg
    19801 ctgctgcggt gaaccggccg ttgtcttgga ccgaactgtg cgaactcctg gcctgggcgc
    19861 atcgggccat ggacgctcgg gagatcattg ccggagtgaa catgccgctg gttctggcga
    19921 tggccaaacg gacccgtttg gcgtccctgg actacaacga actggtcagc gaaggcaatt
    19981 ttgccttgct gcgcagcatc gacaagttcg attgctcccg cgggttcaag ttcagcactt
    20041 acgcatgtcg ggcgattctg aagagtttct ccagggtggc catgaaggcc gcccggtatc
    20101 gcgggcaatt cccgaccgaa ttcgatccgt tcctcgagaa aagcgactat catgaccgga
    20161 aacgggacga tacggcatcg ctgtgcgtcc aggagattcg cgacatcttg agcgagaatc
    20221 gggccgcgct gaacgagatg gagcagacgg tcattcggga acgtttcgcc ctgacgagcg
    20281 gccaggatgg gagcgttcgt ccaaagacgc tcgagcaggt cggacaattg atcggcgtca
    20341 ccaaggagcg cgtgcgtcag atccagaaca aggcgctcaa gaaaatccga atgacccttg
    20401 aggacagtct tgccgcctga cgcgaagggc gacggaaacg gacggcgtcg ctgacggaca
    20461 tggggcgcag caaccagacg ccgcggttcg tcggcaccat caaccagccc gttgcgtccg
    20521 tgaggaagac cctgacggac gtggcgggct ggttctattc tgcgcggcag gcgggaggat
    20581 catcggcgtc ggttgtgccg ggcgatggcc agccaggcca acaggggcag ccagattccg
    20641 atcgtgtcgg ccactccaag tccgaagacg ccgggcagcc tgaagggcag aatgtccggc
    20701 tgcggctgag ggctcaccgg cggcaggttt gcggttatcc ctgaaggttg tgtggcaggt
    20761 cggcgcggag cagccgaggt gcgaatccgg cccgccgggt caaacgcctc atcagatgct
    20821 gcgaatagcc cgtaattcgt tccgataagc agatcctcgt acgacaacgt cagcactcga
    20881 tcatgcggtg ttagaggatg cgagggagcc agcccccagg tcgcaccacc atcgaaagag
    20941 gcccagagca aatcatcatg gcccaccaga atgcggctgg gattgcccgg gtcggctgca
    21001 agagcggcat agcgacgcgc accgttcggg ctgatacctt tccaagacca agcggacgac
    21061 gccgttttgt cgagccggcc gcagaacata ccatgctctg tggcggcgta gagcactcgg
    21121 acgtcgccga cctgtacgcc gtcgatcgca aggaccgcct gcgagtccag actcgtcccc
    21181 gtaagcagct tccgccattc ggcgacgccg gtgatcgaaa gccgcgtgta cagtcctttg
    21241 gcatcccctc gcggatcctg ggggtggccc accgccgccc agaccatgcc gtccaggagc
    21301 gccaagtcgt tgacgggcat gttgagctct tggccggcaa cggccgcgaa atcatcgtcc
    21361 catgagctgc cgttgttgag gctgcgatac accgttccct cgtatttgcc gccgccgacg
    21421 gctgtggcgg cgtacacgag ggtcgaatcc ttgccgacga tcgtgattgc cgtaacggca
    21481 acgccttcag gcgaagagta gcgattgctg aagggaacgt ttcgtgtgct gaagaccgca
    21541 gcccagtgtt ccggatgcct gccgccactc gtggtcctgt aaatcgtgcc ggcgttgtcg
    21601 cccgcgaaca ccacttccag atccccggga tgcatcgtca cctttgtcag ggcagcggtc
    21661 tgcccctgcg gtcgaacggg gaagagccat tcgccgtcga acacatccgg gtaagccatg
    21721 gttttggcca gaccttgact ggtagctgcc cagacgacgc cgtaggtcag gggatcatcc
    21781 gctatttgtg cgacgtcaac gaccgacacg ccgtcgatgc cggcggcgtt gtggatctcg
    21841 cccgcccgcg ccgtggtcat gtcccactgc ccaactgcca aggtggtcga gaagtaaacg
    21901 agattcgggt tcgcggggtc cggggttgcc acgctgccgg tggggtacgc attgacgtac
    21961 ccgagcgacg tcgcaattgt tgtctcgggc aggttgcccc aggtttggcc gaagtcgtcc
    22021 gagtattgtg agccggcgat gattcgcggc cggtcgtgga aggtcacaaa ggcaagtccc
    22081 aggacctggg aggagggtgc tttgacgggc attcttctcc aggtggcccc cgcgtcttcc
    22141 gtacggtaaa ggctggatgt cttgccgccg caaacgacga agaatcggct tgcggtggtg
    22201 gcattccgtg ggtctcttgg gtcgatggca acggcaagga tttcgtcagc aagcgttccg
    22261 tcatgaacca cctggatgtt gccggccaca aagccattgc cgtcgtcgag gcaccgatac
    22321 agcgatttgc cgctcacgac gtagaacaga gtcttgtcat atacggcaat ggaccggacc
    22381 tgctcattcg agaacgcggc cagcggggcg agcatccgac cgccgtcggt agtgcggtac
    22441 acagtaccgt ccccgccgat atacatgccg ctggggtctc caaagagcgt ggttgcccga
    22501 atcacgctct tgttctgcac ccagccgtcc tggtcccatg gtgcatcggg ttcgtgggtc
    22561 aacaaactgc catctctgac cgcccagaga aggccggcgc tgcctagggt gcgatcggct
    22621 tcaatctgaa tatcggtgca agggcctttc atcacaggtt cccagcaggc ggctccgcac
    22681 cggtaaacga catgacgccc ggcaaccgca atgaacagct ccgttgcgcc gtctttgctg
    22741 catgcggcga tatccttgac ctcgccgccg aacagcccga ggttggcgag ttggcccttg
    22801 gcgggtgcgg tggtgatcgc cagtaggatc aagctctgga tcagccggct tggcggcatg
    22861 ttcccctcct tgagccggcg ctgcggcacc aaggcgtgat aagcaataag gagccgcttt
    22921 cccctcgtga caggtaatcg acttgcaggc ggcgaggctt aagcattcgc cgttctttga
    22981 aagtgttttc cctgtgcgac ctggcagcgg ggctgccgcg gcagagagaa gcggcgggac
    23041 cctgaccaac aatcacatga agtctcgcgt aaatgcaggg gcgttgtggg gctggcggtc
    23101 cggtatataa tgctcgcgcg tcaagagaaa acgtgggctc tgaacctatc cgagaatctg
    23161 tgtgggacag gcttccggcc ggtcggcttt cgcggtttct tgataggcct ttggccgccg
    23221 cggcggcggg ggtgatctcg tggtctgttg acaggccgct tcgggtctta tcggtccggc
    23281 caagggtcgc ctttgacttg gagcgtaccc cgaagcgggc ccgcttccgg ccactcggct
    23341 ttcggggctt cgggatacgc tttgggtcaa cggccgtcgc gttcttcgag ggtgtgcggt
    23401 gagcgagcaa ccatcactgt cggttcgatc caggcgcacg gtaagcgtgc ttctgacggt
    23461 tttgtgcctg gtcattgccg cggcgcagat tgtgatcgtc gcgacgacgt acgagtacgc
    23521 gaaccggagc gtttccagtc gcgaaggcgc ggtgccggct gaggatcccg ctgaactcga
    23581 tcgactcaaa tccggcctta tggcggcgga tcctggcagg cgggtcgagt cggccgtcgc
    23641 actgctgcaa cgtggcatga tcgaaggcgg aagtgtcttg ttggaagcgg cctcggaccc
    23701 cgatgtcggg gctcgaagag ttgcggtggc ggaattcggc cgcgtggccc ggccgatggc
    23761 cgaggcggtg ggagtcgttc tcgattggcc ggtgccggcc gatacgccgc ctgcgaccga
    23821 gcacatcaga ctggctaagg ctttctggga gcgatgggcg accccgcggt tacttcttga
    23881 tgtgcaggcc cggctgtcag gggcagacag tcgttgggaa agggtggaac aggctacgtt
    23941 cctgcgaaac tggcttcgct gggtgtcaga gagtgatcgg gacgagcagt agaagactca
    24001 gtcagatcaa agtcatagac catgaagctt ccgccgccaa ttcatcgctg gtcgatgacc
    24061 cctcggcgag cgatctctct tcaacagcgg ctggcgggcc ggatccgcat tgagccgctg
    24121 cggaagatgc ctcgtctggt ggccggggcg gatgtggctt tcagtgccga tgggcggcgg
    24181 tgtttggccg gcgtcgtggt ctgggacgtc gaggcggggg aggtcgtgga agagcgcctg
    24241 gcatggcgac cggcaacctt tccctacgtg ccgggcctgt tgagtttccg cgaagcgccg
    24301 gccgttcttg ccgccgtccg caagctgacc ctccggccgg acgttttcat gttcgatgct
    24361 cagggcctgg ctcacccgcg gcggatgggg ctggcttcgc atgtcggctt gctgacagac
    24421 tgcccgtcgg tcggctgcgc caagagccgg ctttgtggcg agcacggggt cccggcggcc
    24481 aaggcgggca gtaccgtccc gttgctcgac cacgatgaga cggttggcgt cgtgcttcgc
    24541 acccgcgacg gcatcaaccc ggtttacgtg agcgtggggc accgcgttac gctacaggat
    24601 gccgtttcgg tcgtcatgtc ctgcgtgacc tgctatcgcc tccccgaacc cacccgcctg
    24661 gctcacatcc tcgttacccg ccatcgccgc gaaaaatgac tcgcgggctg taccaacccc
    24721 tcaaatctca agtccggaat ctcaaatctc caatctgaaa tctggaatct gcaattcacc
    24781 gcccacgtat catgactcct ccgggcacgt cgaagtcggc ttcgcctgct tactgtcagg
    24841 acactgaatg ctcgccgtga caaaggtggg ggaccgaacc ggccgcaaga actgatgaga
    24901 ctccgtggcg gcacgcgtgc tgcggcatgg cagcgctgct ttgggcagga aacgccaatc
    24961 gtcggaacat cccgagacac ccgtttgaag aaaaaggccc ggggacgccc cgctgctgta
    25021 gtcgccgccg ccacggctcc gaccgcgggc aagttccggc tcagcaccac ggagctgggc
    25081 agcggccatt tcgcaaagtt ccctgttccg gtcctctcgc tccgcccgga acggaccggt
    25141 tgttatgccc tcaacgggat cagcggagat actctgcccg cgatgcgcat ggaccaggcg
    25201 acaggatggc aggacacatg gcggaaggat ttgatcgttt actcatcacc gtacagccct
    25261 gacaagcccg gttttgattc atccttccgc tgtcatcttt tcctgtcccc gctgtccccg
    25321 gatctttggc aattgaagac cgcctatcac aggttgtcag gcccccagcc aagagccgaa
    25381 agctctggtc gtccaaacga acccaacaca agcaaaagcc ttcttcctta cctctcctgt
    25441 tcttggcact gcggccgatc aactggccat tgcgctgaac gaagccaaaa gtgggtagaa
    25501 tggataaagg aggcaatctt gccaggcgcg ggctactggg aatgtaagtc attctatcag
    25561 aatgacttaa agtcgatcaa tgcccacgtt ccagcctcag ccatctcttc accaacacat
    25621 gggctggcag gctctcgcac cctcttctac catgccgcct tgaccggtcg agtctatgtg
    25681 cggtttcctg ttggggagga atgtccatga gccagtgcgg tcgtgactgg ggttgccggt
    25741 cgagtgtctc cagacgcgat gtcctcaaga tcggggcggg tacggccgtt ttgttggccc
    25801 tcggcacgtc ggcccaagcc gagcccggac gcataccgcc tcaatgcaag gtgccgccgg
    25861 gcaaggagtg gttcgatcag cttctgcagc ccggaaagac catcacctac acgggcaagc
    25921 atctcgaaaa gatcatcttt cccctcgggg gtatcggcac cggaaccatt tggctgcacg
    25981 gttccggccg actggtcaac tggcagatct ttaacaacat tcagaagaac agcttggtgg
    26041 acgactcgtt cttcgccgtg agggtccagc aggagggcaa gcctgcggtc gttcgcgtgc
    26101 tgcggcagga gccgttgggg ccgttccagg gcattaagga cattcagttt gtcggccggt
    26161 atcctttcgg ccgcgtctcc ttcaccgaac cggaattacc cgtccagatc gaactggagg
    26221 ccttcaatcc gctcattccg ctcaacgaga aggattcggc gataccgtgt gcgatcttca
    26281 cgcttcgcgc cgtcaacaag agcgacaaac ccgttcaggt gtctttcctg ggctcgctcc
    26341 agaacgcggt tgggcacgca ggcgtggggg cctccgcggg cgtctcgcac gccggctacg
    26401 gcggcaatct gaacgtgccg atccgcgatg agcggatgga atgcgtgttg atgaaggccg
    26461 agccgggcag accagcggaa ttcagtccgc cggttgaact gattgtcgat catcagagcc
    26521 tgccggcggt ggacacctcg cccgtgcgcg gcctgaccct cgcgggcgtg gggcgtcagg
    26581 gcagaaaccc caacattcag accgtgtact ggctggccgt tggagatctc aagcgggtcg
    26641 gcggctcgct gctgaagcag gtggttgagg atgttcgcga gtacggcgca tttctgctgc
    26701 tgtccggcag caagaatccc ctgacgaacc cgttgcagcc gaacctcacg gccggcgtcc
    26761 gcagacgcga gactatcttt gcatccttcg acgatccgag cttcggtcac tggacggccc
    26821 acggcaaagc cttccagcga ccgcggggcg gcacgatgcc cggtcagtcg ccggtcaccg
    26881 gctttctggg cagcggcctg gtcaattcct acgaccccaa cgacgagccc aagggccggc
    26941 tcacttcgcc ggtgtttgtt atccaggaga agtacatttc gtttctcatc ggaggtgggc
    27001 ggcaccccaa ccagtgttgt gtcaacctgg ttatcgacgg caaggtggtg cgcagctcca
    27061 ctggcagcaa cagcgagcac ctggagcggg aggaatggga cgtgtccgag ttcgccgacc
    27121 gccaggctcg tatcgagctg gtggatgagc ggggcgacgg gtgggggcac atcctggttg
    27181 atgatattcg cttcagcaat ctgccgacgg gcgccgtcac ggccgatgag gccgaagcgt
    27241 ggaacgcgtt gctgaacgat gcccggccca gggcaacgag ccggcccgcc ggtgtcggtc
    27301 gcggaaaggt catgctgatg cccatggacg tcgacgccac aggatcgcct gtcgaaaagt
    27361 tggccgtgcg cgacaagctg ttgtcgatga tcgcctcgct gacggatgtg acctaccggc
    27421 cgcaggtggg caggcagccc gacgcgccgt cgttcggtac catgtgtctg gcgacgccgg
    27481 agaaagacgc gaccatattg cacgactgga gcgaccgcga tgtgctctac cgcctcttgt
    27541 cggattccgg tcggctggcg gatttcaaga ttccgcccgc aacggtcggc cctaccggtg
    27601 ccggtcagac gctcaacggc gccctgtgca tcgagagagg tgttgcgccg ggcctcagcg
    27661 ccgaggcgga tttcattttc acatggcact ttcccaacca gtactatccg cagaacagct
    27721 atcagcagag cggtcgtcac ggcgtggccg tcggcaatat gtatgacaac tggtatcgcg
    27781 atgccctgga cgtggcccga tatgtggtcg ccaacctcgc cggcctgcga aaacacaccc
    27841 ttgcctatcg cgatgccatg tttgatacca cgctgccgca gtatttcatc gacgcggtgg
    27901 cggcgaacgt ctcgatcatc cgctcgccca cgtgtttctg gaccaaggac gggactttct
    27961 acggatacga gggttgcagc cccggtggcg gcgggtgctg cccgatgaac tgcaaccacg
    28021 tctggaacta tgagcagtcc ctcgccaagg tgtggcccgc gctggaccgc gacatgcgca
    28081 ggaccgagct caagtttcac cagaacgagg ccggcggcac gaatcaccgc gtggctgttc
    28141 cccgcgacaa tccgcacaag acggccccgc ccgtggccga cggacagtgc ggggcggtgc
    28201 tgaaggccta tcgcgagcac ctccagtcgc ccgaccgtcg gtttctggac gagtactggg
    28261 agcagatcaa gaaggcgatg gactacgcca tcaagacgtg ggacaaggac ggcgacggcc
    28321 tcatggagca gccgcagttc aacacgtatg accgcgacat ctacggacac aacacgttcg
    28381 tcacgtcgct ctatctggcg gccctgcggg cggccgaaga gatggcccgg ctccgcggcg
    28441 acaagccggc ggcggacaag taccggctgt tgttcgagaa gggtcgggac gcggccgccg
    28501 gtctttttga cggcgagtac tacattcaga tcgccgacaa catcaacctc gggtacggca
    28561 aggggtgttg ggcggatcag gtggtcgggc agtggtgggc ccgcgtgctc aacctcggcg
    28621 acatcctgcc tgacgagcag gttcgcagcg ccctcaaggc cgtcttcaag cacaactggc
    28681 tctggaagat tgacggtttc gaggggacgc agcgtttcct tgagttcgcc gacggcgacg
    28741 ataagggctt gctgtgcggg tcgtggccga agggaggacg gccgccggac cccatcctct
    28801 accgcgacga gtgctggacc ggcgtcgagt atcaggtggc ggcccacaag atctacgagg
    28861 gccaggtcga ggaaggcttg atcatcgtcc gtggtgcccg ggaacgctac gacggcacga
    28921 aacgcagccc gtggaacgag attgaatgcg gcgattacta cgtgcgggca ctcagcagtt
    28981 ggtcgctgct gctggccgct caggggtatg cctatgacgg gccggcccgg cggctggcgt
    29041 tcaatccccg catcggcccc gaggaccatc gctcattttt cagcaccgcc gagggttggg
    29101 gccgattcga gcagaaacgc ggcggcaacc ggcagaccga cgtgctcgct tttgattggg
    29161 ggcgctgcga cctgagagaa ttgcgattcg gcctgccgcc cggagcggaa aaggtgaccg
    29221 ccaaagcccg actcgatgac cgcgacctct tcatcgaaac gaggctcgat gaccaagatg
    29281 ccgtcattat cctgagcgcc cccgtcaccg ccgtggccgg caacacgctg acggtggaga
    29341 tgagctactg ataatggcgg agtccgcgcc ggcaggcgta caggtttaga atggtataag
    29401 gctcttttag agtgtttaat cctgcatagc ctcttgcgcg ttgcggccgc gatgatggcg
    29461 ggcgccaaac atgcggcccc aggcctcagc cgcggcccgc gggattcgtt tcgtcccgaa
    29521 aaagaagtgg ccgacccagg gccggccact gccgatcgtc gaaagctgac cgcttctaat
    29581 catccatcat gttgtagtag ctgatcggcg gcgtctcggt gatccgctcg ggcagctcgc
    29641 tcgggttgta caggcaccgc gtgcggtcca cggtcacctg cgtccgcacc gtcaccggcg
    29701 gctgaccggt gctctggatc gtcgcgtaga tcgtgaacac gtggttcttc accgtcatcc
    29761 agtcctgcat ccggacaatc ggcgcgacca gttgcaggta gccgaggtaa ctgaacggcc
    29821 gctcgttgtt gtcgtccgcc caacgccgcc attcatccaa aggaaggctg ggccccttga
    29881 tatctgcgcc cgtcttcggg ttcttcaggt cacctaattg taccttcgtc agcacattgc
    29941 agatttcgcc cgtggtcgca aagccgatcc ggtcggcata taccgcctga accgccccaa
    30001 ctggccgctg ctcccgataa gacaccatat actgagccag ccgcggcgaa atgctgggca
    30061 tttcggcggg gggatcgaca taataattag gagtgctgga caggggttgc ggcagtgttc
    30121 cggagtactt ctcgtcgatc aggcgatgca cgaaccaagc ccccgccata aatttcggat
    30181 ccgcggtgtg gatgtacggg tcaagccgct cggcctcgat ctccggcacc ggcaggcatg
    30241 caaggttgta tgtccaggcc ggattcacat ttacagaatc cgtgggggag aaatccggca
    30301 gcaccggcag gccgtcgagc acccaccacg gtgcgctacc gatgccgatt cgcccctgca
    30361 ctcgcggccc aagagtctgc gccaccggca tcatcaccgg gtagttcaag aaccgtgact
    30421 tggtccagtc accccagtta tctgccccgc cgacggcacc atagtggttc aggtaatccg
    30481 tcgaatccat cagccgctgc gcacctggcg tgagaggacc caggtcgcga gccagttctt
    30541 ccatcggcag tgccgtgaag tactcgaacg ccaactgccc ccagggcagg ccgccaaacc
    30601 gcgagttctt gtcgtcgagg cacgcctgag ccgtgtcaaa gaccggcaga tgaccgttgt
    30661 caagttgcag aatctgttct gctgctgacc gcagggggat caaattcccc aaggcatcga
    30721 aatccctgac gacgaaatcc atcgcggcaa cgctcagcgg cttgtcctcc gtcatgattc
    30781 cctgcatggg cagatgagca taccgcgtca ccagcagcag cgttccggtc gtcgggaacg
    30841 ccatcgtgcc cccggtgttc ggatgcacgc cgcgatcggc caccagcacc gggaacgggg
    30901 caattggaaa cggcttaact gtgatttcgg gttgcacact tgccgtaacg gcgctcacca
    30961 gacgctgccc tgcgaactcg gcggcgagat aaccctgatt ggtgagaaat ctcgtcgtcg
    31021 cacccaccgt caccacgttg gcaaagtctg ttgttgacgg cgcggttccg agcaggttgt
    31081 gttgcggcgg cctcgtcgag tcacaccaca cgcgtgtttt gtcacttggg tccagcaagt
    31141 acacgtacgg gtcggatgcc gcagttggtt catccttgtc ctcctggtac ttgagtggga
    31201 aaaccatttg ccgcgacagc gtgaaatgcc agtgtaccgg ccgctccgga ttcggttcct
    31261 tatgacgctg caggctgcag tcccagacaa agatgtcgct tttgttcctt gggttccgca
    31321 ggttttgggg attagcaggc aaccacttgc tggaatcctg cgcggtggcc caagccggat
    31381 catcaattgg ggccagccgg tcaatctcga tcggatcggt cgagagcacg ccgttcggat
    31441 ggtcacgatc taccttccac gtgccgctcc tgtacaagcg aacaaatctg tgcaggtcat
    31501 ccagcttcag tgcactgttc tcgacgattt tcccgggcga caccggggtc agaaagtcat
    31561 cgccgctttt gctttcatcg gccttggtgt taaccaccag ataatactga tgcggcggaa
    31621 tactctgcgg caaggcaggg gcaattgtcg ggtcgatgac gtcgactgcg accgtccctg
    31681 tgccggaggc gtccgtaacc cgaatcctga agcttgggtc ggggactgta ccgagttgga
    31741 tgggtgtatc ataaggattg tacagctcga ctgcgtagat cgacaggtcg gtaccagtgt
    31801 cccaggccgc tccctcgcca tcagctttgt agatgaactt tgcatacgcc tcggtgataa
    31861 acggctgctt ctcgacgccg acgaccttcg ccgcaagccc cgcgccgttg tcgaactcaa
    31921 ggtatgtggg aatctggtct gcatcggcaa aatcgatcgt gttgaccgcc agttgcatgg
    31981 ccgtgcgcag ttgctcggcc agttctgcgg gcgtcgggtt tcgaagatcc gacgggttcg
    32041 gcacgttgct gcccggcacg gtcgtgtgct gaatcatggc caggaagtag gccgtcagtt
    32101 gcgtcgcgcg acggtaagaa gcttggccgt tgggttccag cacatcccgc aggctgaact
    32161 gcgtccgcag accgtctttg ttgaacacat gatcgacaga gccgccggca ggcccgtagt
    32221 ccatgacgcc ataggccaag gggggacgat tgggatcagc gctggcaggg gtaacaaggc
    32281 tcgcgtcgac cggactgatg atgttttcga gattgaaggt aacggtatcg ggggcaacgc
    32341 ccaacgtaaa ggtgaaagcc ttcagtctct tctcttcccg ctgcggccgt agggcgtcgt
    32401 cggagctttc ggtggtgatc aggtgccggc ggtcgtaagt gtcacgggcc gcattgtagg
    32461 ccggatcggt aagtccgttg tacgtcggct cgatgcgacg ctcccagtcc tgctgttcct
    32521 tatcgctggg ccggaacatg gtccaccgat tcacaggttg attgagccaa ccggtaggct
    32581 tggccggatc cgcatatccg gtggtgatcg caaggttctg taccagaact gaggccacgt
    32641 tcttgtaatt gcccgagtcc ggctcgggca gcagcggcgg gagcatgaac cgccggcgca
    32701 gccggttctc gtccgtcggg ccgagaccca gccccacgat ggccgtccgg agtgcatcca
    32761 taccacccac ggccgcgaga tctgtcggat gaacgacctg tgccagcagc gtcgggtggg
    32821 tcatgcggtt cagccacacc atggcgttgt gcgggatgac ccgcagactc atgtggtaag
    32881 tgccgccgaa ggtgtcgctc aaacccagaa tgtcgtttat ggcggcatca cgtatgccgt
    32941 cgccgtcggc gtcttcgagc gggttatcgg tgatgaagat attgaagttc ttgcggctgg
    33001 cgttcggctc gcccgggaaa tcgctgggcc aggagacggc aaagaacctc cagcgatcat
    33061 ccgccgtgcc ggggccgccg ggatcgaagt acggttccag actgctgagc aacaaatcgc
    33121 cgtttcgcgg agaattggcg gctatgaagc cggggtagtc accgtaatcc tcgccgagca
    33181 aggtgttgtt ccaattgtct cgcagatatg ccttgttgtt cgagccaagc aggtcctgcc
    33241 gcaacgcgat gatcaccggc tccaggatac cgttgctgac ggcctccatg cttcgggcgg
    33301 tcatactgat ctgcaccgtc tgacgttcaa accggccgac gatcagaagc gtcgatccga
    33361 tgataaagag catagccaga acaccgacca ccatgaccat gacggttccg cgtctagcgc
    33421 tcgctttact ttttgctttc ggggcgcaca tcagcccaaa tcctccctaa agataccaag
    33481 agaatatacg ggggactaag tgacgagggg aggccagtgc gtgattccaa ggggacgcat
    33541 cagcggcttc ccaacaggag cgaccctgcg gatggggaga atggcccctc cgaattgtct
    33601 tctgaagcgc catgattttc ccgataggga gcttgggggg gcgggcgacg gattatcgca
    33661 acgcgaccga aaagcctcct gctcggccca cttggcctta tggcggaaca gcagggggtt
    33721 ctatgtgacc aggttttgcg ccaccatcga gtggtgccgg ccgagttaca gtggcgcagg
    33781 atttgccgtc gggcgaagtc agcgagcaag cggctatgac gccgcttggc acgacatgcg
    33841 gtcgcctgcg aaagataatc tgatattgag tgctactttg tgcttgaatt ccgctttagg
    33901 ctcgtgtatg cttctagtga aggagtaccc cggcaggccc ataagtgtgc aagccattga
    33961 ggttttgcag cgctgccgat ggccgtggct cagtcccgat cgcagcatgg caaacctcgc
    34021 cgataagaaa tgacctgagt cctgctgacg gccgccgaac acaggcctcg cctccggggg
    34081 atcggtcggc acggtcagcg cgtgaccgga tgcggactta tggtcggccc cgaggtaagg
    34141 aagaccatgg gagcgcggta gcagggcatt gctggtctgg taatgacagg aggctgttga
    34201 tcatggtagc ccggttccag aaacgagatg gtgttcgcgg tttcactctg attgaagtac
    34261 tggtcgtggt ggccatcatc gcgctgctga ttgcgattct gctgccgtca cttgcgcggg
    34321 ccagggcgca tgcgcgcaat gccgcagatt tgagcaacca gcatcagatc ggcttggcta
    34381 tgaatatgtt cgccagccgt aataacggca aagtgcccgt cggagcggat cccgacgacc
    34441 aggaccattg ggtcagcatt gtcgcccgcg agatcggatt ggtgaagcgc ttgacctcag
    34501 acatgtgcgt caaccacatt cgcgtcgacc aaattgatct gtaccagtgc gccgagcgca
    34561 ccgcgaccgg ctccgcgccg ttcctgggct atgtcagcaa ctcgctcaat cccgacggtc
    34621 cttcgggcac cgactggcgg ccccttgacg gtgttgtgaa tgtggcaagc aggtacaaga
    34681 cgccggccaa cgtcatttat atcatgtgtg cggagaccga accgaaggtc tgggatggca
    34741 aaaacagctt gggctacctg agcccgttag gggctcggaa aaactgggaa agcgcggttg
    34801 cagcgggcct gttggcaccg ggtcgcgaag cccaactgaa gagcactctg atcaacagtg
    34861 gcgggggcct tgattgcctg gatatctggc gcgggggtca attgccgcag tacaattgcc
    34921 cggatattcg ccagcccaat cccaaccgtc gtgtggcttt cgagttgcat ctgaaccgct
    34981 ttaccaacgc gatgttctat gatggtcatg ccggaccggt gcctgcggag aagcggtcga
    35041 cctggcagga gaaccaccgc gtctggctcc agaggtgcgg cctgacgccg gcggggatcg
    35101 ctgctgcgtt gaacataccg tgaagttctg tggattgcag caagtgaagt tcttgaggtg
    35161 aggcggcgcc aagcagcatc ttgtgtgcct gacttgcccg cgctctggga tgacccccgt
    35221 gtgccgggat gtggttcagt gggattggcc ggcctcgagc gcgcgggagc atcagagtca
    35281 caaacgaaat agcaacactg cacgcacggc aacttgcccg ccgaagaagc tttccgttct
    35341 tcagattgca gatcaccttg ggcgcgacta ttctgacggt ctatggctga cagtcactcc
    35401 ggcgaatgcg tcttcagcgg ggtcggaggc gagagcgacg cgctcgtcat ccgtgcggat
    35461 ctgccgggcc gctggtacgt agctcacacc cgtgcccgca atgagaaaat tctggccgag
    35521 gaattgagcc ggtttggcat ttacaattac ctgccgcttg ccctgcacgt cactcgcagc
    35581 agccggacca atcgaatctc acgcagcctc attccgatat tcccaggcta tgtttttttc
    35641 catggctccg aggaacagcg gtatctggcc ttgcggacca accgtgtggc caacgtgttg
    35701 gatgtggtca accagaacca actcctggcg gagctgagac aggtgcatcg tctattgcag
    35761 gagcggggcg atttcgcggt cattccgcgg atacagaaag ggcaatgggg acggatcatt
    35821 gccggaccgt tggtcgggct tgagggtatc gtggtgcggt acgccggccg ttatcgggtg
    35881 tgcatgaacg tgaccattct gggccagtgt gtcggcgttg aggtggatta tgacctcgtg
    35941 gagcccatcg acgcccccgc ctacctgacc acccgcccct aaagcccagg tcccaacaga
    36001 ctgctagatc actagaccgc tggcccaacc gcccaccagc ccagcagccc aataacgtaa
    36061 tggcccaaga gcccaaggcg gaccgccttt ttgcgggccg caacccaaac gttctgcgat
    36121 ccagaaacat gcgcgcaaaa tgcacaccag tcgcgacgga agagactaac aggctagccg
    36181 actagtagac cgcccgcctg cgaacgcatg aacggttcgt gacgtattat tctccaaaga
    36241 aggagggaca gccacgtcgg gtctggcgct gaaagcacta caacgcacag gttccgtttc
    36301 tccacggcgg tcgccttcga gccggctgtt ttggatgggt tgctacgggc gcgggttgtg
    36361 gtgctagtta tggaaaataa acgagatagg cgcacaagag cggctgttgt cctgctggct
    36421 gaaggccgat agtattaatc gtgcgccagc tccaggagcg aggctgcctc cagtgttact
    36481 gaatttcagc cggatgcgaa tgacggctga cggggacgga gtgtgcgcgg accatgttcg
    36541 tcggggcggg tgggtgtacc ggggtgtgtg cctttctgct tgaccgggct cccgcttggc
    36601 ccggaattcc gcttggcccg gaatccgttc cgggcctatt ctgcgaagtg ctgctcggcc
    36661 cgaaattccg ctcggcccgg aatccattcc gggcctatcc tgcgaagtgc tgctcggccc
    36721 gaaattccgc tcggcccgga atcctttccg ggcctatcct gcgaagcccc gctcgccccg
    36781 gaattccgct cagcccggaa tccgttccgg gccggtcccg cgaagcgccg ctctgcccgg
    36841 aatcccgctc agcccggaat ccgttccggg ccggtcccgc gaagcgccgt tcgccaagca
    36901 ggtccgcctg gtctggctca atccgcctcg caacaaacaa aaaggtgaag gtagctgatc
    36961 ccctgccatt cgggagtctt ttttgaaaat catcaacatt tgcggcgccc ggcccaactt
    37021 catgaagatc gccccgttga tgcgggccta tcggcggtat ccggccatcg aggcgatgat
    37081 tgtccacacg gggcagcact acgacgacag attatcagac ttgtttttcc gcgagctgga
    37141 gatcccgaaa ccggatatca atctggaggt cggctcgggc agccatgcgg tacagactgc
    37201 cgagatcatg aagcggttcg aaccggtggc cctggaacag cagccggact gggtggtcgt
    37261 cgtcggggac gtcaacagta cgatcgcttg tgccctggtg gctaagaagc tggggatcag
    37321 ggtcgcccac gtcgaggccg gtctgcggag cttcgaccgg acgatgcccg aggagatcaa
    37381 ccggcttctg accgatgcga tttcggacct gctgtttgtc agcgagccga gtggaatcga
    37441 gaatctgcga cgggaaggtg tcgacgggga taaggttcac ttcgtcggga acgtgatgat
    37501 cgacacgctc aggagcaacc tcgagaaggc cgaccggtcg agcactctga gcgatctggg
    37561 gctggagccg gggcggtata acgtcgtgac gctgcaccgg ccgagcaacg tggacaacgc
    37621 cgagtcgctg ggccggatcg ccgatgccct ggaggtcatc cagcaggacc tgccgacggt
    37681 tttcccgatg cacccgcgga cgcggaacaa tcttcagaag ctgggtctgg ccgagcggtt
    37741 tgctggtatg aagcagttgc ggatcgtcga gccggccggg tacctcgatt tcctcaagtt
    37801 gatgggtcgt gcggcggtgg tgctgaccga ttcgggcggg atccaggagg agacgacgat
    37861 tctgggcgtc tggtgcctca cgctgcgcga caacaccgag cgccccgcaa cgatcaccaa
    37921 tggcaccaac cgcctggtcg gcaacaaccc ggccaaggtc atcgccgcat ataaagaatg
    37981 ccgcaaggcc cccccgtcca aaaccatggt ccccgaaaaa tgggacggcc acgccgcgga
    38041 gcgcgtggcg gaggtgatgg tgggggggct tgaaaagtcc gctggctgtg ccggttgctc
    38101 atgccatggt tccggtgggg cagtgcggag gtgagcagtg gggagatttg gctgggctca
    38161 ccgggctgca atcggctgtg ttgttgccgg gatgctcgcc ggtcaggcag cagtcgcttc
    38221 ggccgagccg tatccgttgc ggtggttcta tctcgcttcg aattttgctg tggcgcagaa
    38281 tgcagaccgg gccatcgcgt tgattgagga ggcttctcgc gcggggctca atggtgttgt
    38341 tctggcggac gcgaagttcg cgacgctggg caaatacaac catgactcac cgtcgaatcc
    38401 ctactatcgg aacatccgtc gcgtgctcga tgtctgccga cgaaacgggg ttgaagtcgt
    38461 cgtttatttg cctaccatca gcaaggcgca tgggatactc gcacacgatc ctaaccttgc
    38521 cgccggctac gaggtacgcg atgcggtgtt cgaggtgcgg agcggcacag cgcggttggt
    38581 tcaggatccg gcagtgaggg tggcgaacgg aggcttcgag gacgcagtca accagcgttt
    38641 tcgaggatgg gacaggcagg acgatccagg ctcggcggtg gtagctgacc gaggcacagc
    38701 tcgcatgggc gtcatgtctg cgaggctgca gtttggagac gctcggcggc tggggcatgc
    38761 ccgtctgggg cagcggctca aggtcgcgcc ccgacgctgt taccgagtga gcctctggct
    38821 gcagacaggc ggattggctc ctacggagtc cttcaacatt ctcattcggg gtgcccgggg
    38881 cggccaagtc cgcgtgttgg cctacaaccg cttcagcgtg aaggccaccc agtcgtggac
    38941 gcagatggag accgtgttca actcgctgga ttatgaggag atcgacgtct acctggggtt
    39001 gtttggggtt cggagtggcc gggcatgggt ggatgatgtc gaactgaccg aggttggcct
    39061 ggtgaacgtc ttgaggcgac ccggctgccc gctgaccgtt cgcagttggg acgggcgtgc
    39121 aacatatgtc gaggggcagg atttcgagcc ggtctacgat ccactgttgg gcaaggcggg
    39181 tgatcgggcc ggcatcttcg accgcttcca tcccgccccc gatatccggt tccgcaaggg
    39241 agtgcccgac ggcacacgct tacgggtggg cttctatcac ccgctcatta tccatgcgaa
    39301 ccagatcgac tgctgcatga gtgagcagaa agtctttgac attctggagg acacgatccg
    39361 aaggctgaat cgcctgctgg attcgccgaa gtactggtgg ttgggctacg atgagctccg
    39421 gacaggcgga ggctgcaggc tgtgcaaggc ctctggcaag acgccaggcc aactgctggc
    39481 cgagcatgtg cggcgggtgt cggagatggt acggaaagag cgacaaggag cagagatcgt
    39541 cgtatggtac gacatgtttt acccgggatc gaacgccaaa gacgaatact acttggtgga
    39601 agggagccta acgggtagct gggaggggcc ggatccgagc gtgatggtct cgaagtggag
    39661 cgtgcttggt cgtgacgaat gcatgaggtt ctttgcggat agaggtcacc ggcagatcct
    39721 cagtgccgat tttgattcgg ttaaggatcc ggccggcttc ctgcagagct ggctggagag
    39781 catggatgct gtgagtggcg ttcagggaat catgtacacg acctggaccg gtcggtatga
    39841 ctacctgtcc gcatttggcc ggacgctcaa gtctcactcg ataccggcat ctgccagtcg
    39901 ccctgtgccc aactaggtgt tatcggcggc gggaggcctg cttcgacaga tgagtctgac
    39961 gcgaaggacg gcaaaacacg gtgtccagat ggtaggcagc cgattgctcg gaatgggcat
    40021 cggcgt
//
LOCUS       DAOTPW010000012        39642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1108_length_39642_cov_5.777301, whole genome shotgun sequence.
ACCESSION   DAOTPW010000012 DAOTPW010000000
VERSION     DAOTPW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 39642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39642
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_39642_cov_5.777301"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="P5316_01890"
     CDS             complement(<1..403)
                     /locus_tag="P5316_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83737.1"
                     /translation="MRRRSELAIILSMSAVILGRIQEAGAVLNNGFETDYVYVTRDIA
                     QGGQLRVYRELDGTQVADLLPDGTNWETLTFAGTGGDDARLFVAKVVDNDVQVAEIDS
                     AGGTVNSANLSNIIGGTVGTAPYMGNMRYSSV"
     gene            complement(514..1638)
                     /locus_tag="P5316_01895"
     CDS             complement(514..1638)
                     /locus_tag="P5316_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR83738.1"
                     /translation="MKIRELATVVVALGVITSLAPNVWGYLNNGFETDYVYVLRDTGQ
                     GGQTRMYRESDGANMGNLLPDGSNWTTLTFAGSGTNDARLFVAKMTGGDPGTDIQIAE
                     VNALGQTVKSVSLSSIPGVTLGSGLRLGNIRYNRMHNSLIVGANPDTTVIGVNAKAWE
                     IDLGLSQLQHTYVGATLPNSAGEERPVNVDFNERDGSLYMISRHLGRPTADGLGNLIS
                     FNTIGRVLGGTTNVYSTLIDGAEYAVGDAEYSQPNNVMFRGTNNPSGRPTILIPSNQT
                     SGWEPTLEFYLDEIDSNGNLVNRGQPIWAKRGWNGQLDEVSGTVWFGAFRGGIMGLNP
                     DDTTSYWEGSRYWLDADSPAPEPASLILLGLGLLGLRRRR"
     gene            complement(2137..3537)
                     /locus_tag="P5316_01900"
     CDS             complement(2137..3537)
                     /locus_tag="P5316_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83739.1"
                     /translation="MVVREPCAGRECLPPDGQGHIPTQRESRANRNGALLRRGRTWVL
                     RFTGTRPGVWQYTTASKDPDLNDRKGELTVEPNPGLAGFTVAFGNKWGRLGLDEAFVP
                     QFVSYCGPADYFNRPDKIDADIRTWFEDHGFNGFHTFVGMAWFDINKGQGNYRDIPSD
                     DPNPDPRTFEALELLISKAHKNGGVVHIWMWGDEQRKITPVRWGMNGSVDRRLQRYIC
                     ARLGPLVGWSMGYGFDLQEWAGREHLRLWHKHMHQHLGWPHLLGGRSPDLEQIYDGLD
                     YSSYQQWRPTYDTYVEAMEKRSPGKPSFMEDRFRVRVNVYPEKDYDLDMTRRGLWNST
                     MAGGVANIWAYLVNPPRDGSSAVYPNKEQIRTYSRFWKSRFHKQMVRNNARTDGVCLE
                     ITDRFCVFYKEDADAIRMNLAGLTEPLEAVALDTKAVYREIPLTGLKAAPDQVFMAPH
                     NSDWALAIGNPNPSPR"
     gene            complement(4083..5300)
                     /locus_tag="P5316_01905"
     CDS             complement(4083..5300)
                     /locus_tag="P5316_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HRR83740.1"
                     /translation="MPAIVDFPTIVKEALNEFGHLFANEPERIHFAEYLTGLLVADRK
                     NVSAINRQFVVTTDQSCLNRWLTEVDWDVHRLNQQRLKWLQKDPSTRYSPQGVIALDN
                     VLVDHDGKLIEDVGYFWDHAEDRYKIAHDYLIANYVCTSGKHYPLEFRRFVKREQCEK
                     QGLPFRDHNVFFRELVDEVIRHGIPGDFTFDNWFTHAANLNHIHRRRRGYVGDLKFNR
                     KVVFRGQTIKAEAVAALIGPEDRKPVDIGDHRQWYFTKSIRIPQVDHPVRIVILWERR
                     NGAHPRKILVSNRTYWEVSRILRVYRCRWRGTECFHRDGKQHLGMGDCQLRKGQGQTR
                     HWYMVFLAHSVLMRQLRQGRARDWALERLTTIGQACTAVLRQTLSQTISWVIERVEEG
                     WECNRIKAHLALA"
     gene            5402..9115
                     /locus_tag="P5316_01910"
     CDS             5402..9115
                     /locus_tag="P5316_01910"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83741.1"
                     /translation="MCFKGLIGKQSFRFGSLISFTAAVTLTVTTFLCLTGCRPGDGNP
                     SDGTYTITVTVNPAEGGTVTIDPAEGPYTAGQVVTLTAQASAGYEFDRWQGSISSTAN
                     PAQVTVDDNLVIEAVFVATGNGGGGGGATDTEINENVRVLPDTVEVTTDDEGHTTLTG
                     QGIPTGIAPGALLVSARGEGRLLRVKSVPTVGDGQVIVETEQASLADLIRKGSLEISV
                     PFDPNTAVLQGFSGVGSGDMKHVRFLRTGDRSAAFTTNSHSIELEDWGKVTLDQLTIN
                     VDPDFSFTASIEDNSLKHLRCTAGTGFHLSMDVSIEAYKISELLKKEQTIGKVKWPDP
                     VGVMVIAGVPVVYQVFLEVKVGAQVKFGDMGAITAGLDFDADATLGAEYNKGEGWDLI
                     NEKSTAFTPHTPEWGISPITAQVYVQPEFGMTFYGCVGPSLSWKEYVELAGAYRYDVL
                     GAELARGSECDFNLTFKIIDGLPSYKYSHNLFSTRQVLLARMLTAVDPAELGSASLSP
                     GDLVWDLYDFTKSLTATARPNAGYEVAGWTIYNLLSGSGETVHASQLHNQPVNASKLF
                     VAGIVPEGYGDTWTGAGTGSGAPSYALTTEVFPPEAGEIMLFPGGGVYQYGVNVLVAP
                     KPNSGFEFHHWEGALSGNGPTGVISMTSSRTVRAVFASNPPRTLYVPSHYPTLQDALN
                     AATYNDIIHIEAGTHAGTGFHDISIPRDHIVIQGAGPSASIIDLEGVAQLGWLDDIHI
                     TFQNLQISRGLGDLGGALRLTGDTTASFKNCTFSSCNASYGGAVYVSGTGAIVSFENC
                     RFESNQATSSGGALSLSNTPNDEEHVPVVAGCTFTGNMADSSGGGIVAGQKCRITGCT
                     FAQNRAWRGGGGSFGKEGVVSGCVFDGNSAQYGGGVSADEYSSFSECSFINNVAEWSG
                     GGVKAGNGVSIQYCTLDNNQAGEDGGAIYADCVSAGKTLFIVGCAVSGNSAGENGGGI
                     WCDGTEVSQTELTGNSAGWRGGGICADHSQISSCFLYQNQAGAAGNPSYSEGGGIYLE
                     QTNVSNCQLIDNEAIDGNGGGIYAQGSFSDTAVPPVIFEVYAAFNTAKNGGGMGSDLE
                     VHIFSSIFEDNEASYRGGGIYAERGPLVSLCRVEHNSSQQGGGAGISEGTILASTITR
                     NQASQWAGGLSLFKSTAQDCTVSHNTAATAGGGIWTGIECSISNCTVAYNALTDTTST
                     GGGGIACHGAPTLSGVTVYGNTPTNCNSDCPCP"
     gene            complement(9485..10144)
                     /locus_tag="P5316_01915"
     CDS             complement(9485..10144)
                     /locus_tag="P5316_01915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR83742.1"
                     /translation="MSKAETMRSQARREFDTWAGSYDRSLLNRFMFRPSYLAFLEELY
                     LWRKDTPEPFHLLDVGCGTGTFAGMLAATALPARIVGLDYSAAMCQVAADKARPTGLQ
                     DRIRFVTADSEHLPFADGSFDVVTCGNSFHHYPHQQNVVTEVRRILRPGGRFMLIDGF
                     RDNIVGWVTYDVIIAAVEKHVHHAPWSVIDAYFKTAGFREIRRRKLNYWFPLLLTVGM
                     A"
     gene            10305..11666
                     /locus_tag="P5316_01920"
     CDS             10305..11666
                     /locus_tag="P5316_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRR83743.1"
                     /translation="MPKRNLVWIAVGAVLAVLLWKVPEALIRRDALYDQFGPLTDVSL
                     QIHRNYVEEVDKKTLLRGAIDGMMQRLDPYSRYFDSAEYEQFSKRIEGQFYGIGVEID
                     QLHTGELVIVSPIEGSPAFLADLRADDRIIMIDDTRTVDISAEKAVELIKGEPNTSVR
                     LTLFRPSTGQQFEKTIERKLITVPTVRGWAHTEDWEWDYLIDPEYRIGYVRILNFELN
                     TAKQLDVVVRKLLVYDEIQGLVIDVRDNPGGLLDVVVPVADRFLDEGIIVSTRGRNTP
                     EQRREASRQDTYPSYLKVALLVNGGSASASEILAGALKDHKRAVVVGEKTFGKGSVQT
                     LFEVENGHGIVKLTTGYWYLPSGERIHDKGIMPDKVVELTPGERAAMIESRVRVYSTR
                     RPSLPATATAPATASAPASRPSATSTATAPAKITVMIDRQLQTALDILREQIGPAATA
                     PAR"
     gene            11681..12190
                     /locus_tag="P5316_01925"
     CDS             11681..12190
                     /locus_tag="P5316_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRR83744.1"
                     /translation="MEKDPQQVARLAEKRRDENWRFRSFLKMLSRRRQREVNRIAEQY
                     GLEAESRIDCRECGACCRDNAIPVSEEEIERLAGRLGLSPDEVRAKYISHDEAEGDHI
                     DARPCPFLNGNLCSVYDSRPEGCRGYPYIGGDIATRTIGIIERAGTCPIIFDMLERLK
                     DAVGFRRYR"
     gene            complement(12181..12444)
                     /locus_tag="P5316_01930"
     CDS             complement(12181..12444)
                     /locus_tag="P5316_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83745.1"
                     /translation="MDLASRLESLLTLAEEIGVDVRAEPMGGDGGGLCRLKGRTILFV
                     DTTADVATRYDRTVAAMAAMPELADRYLVPEVRQDIEHYLAHR"
     gene            12518..13405
                     /locus_tag="P5316_01935"
     CDS             12518..13405
                     /locus_tag="P5316_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR83746.1"
                     /translation="MALQPQKIPVRTPPAREGVKPPPRPATPAAAPRPASEVSTLAKD
                     AYALQAQVDELARQFEVMQAQLRQTQKLASLGTTAAMIAHEFNNLFTPVVAYAQQALD
                     TGDVELMKTALAKTLDRTNAMRQMADRVIGLARQPSGSIRSVNVRQLADNAVGCLCRD
                     LGKDNISLNIQIDSGLCVRADENQLLQVMFNLVVNARQAMLGRRGRLTIDAVPIDENR
                     VEINVRDTGCGISPENLGRVFEPFFSTKQEADKPDRRGLGLGLAISRNIIEDLGGTIS
                     VTSEVNVGTTFTVTLPRAE"
     gene            13897..14535
                     /gene="nth"
                     /locus_tag="P5316_01940"
     CDS             13897..14535
                     /gene="nth"
                     /locus_tag="P5316_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRR83747.1"
                     /translation="MMRILAGLKAAYPDADCALKHGSALELLVATILSAQSTDEMVNK
                     VTPVLFAAYPNAAALASADPADVERIVHRTGFFRQKTKSIINACRMIVERFGGEVPQT
                     MDELTQLPGVARKTANVLLGTWFHKNEGVVVDTHVGRLAHRLGLTWTSKDEKDAVKIE
                     QDLMQIIPREEWTYVGHALIQHGRRVCSARKPNCVGCNLNKHCPSAFAFGEE"
     gene            complement(14610..15164)
                     /locus_tag="P5316_01945"
     CDS             complement(14610..15164)
                     /locus_tag="P5316_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRR83748.1"
                     /translation="MLIAESLSPESVVLCETAGSTIEVVKKLAAKLAGQLGQDRQTIE
                     EAVIARERARTTAFTNGAAIPHCRLAELHRFGIAMMILKKPVRWDNQGHAVDIVMMIA
                     GPSQHIPEQLRILANSGQVLDSPAVRTKLKRAPDAKAVCELMAAAEKAVEARRARDGV
                     LRELRRDQADSADYLAEVADTFQW"
     gene            15607..18852
                     /locus_tag="P5316_01950"
     CDS             15607..18852
                     /locus_tag="P5316_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83749.1"
                     /translation="MNAMLVLTMMAGTAVVDKRIDLGEFARAVEYTGDKPETLEVREL
                     DRGIDGWTAWKGEDGQYMIGLEWDEPRDIAETCIEFRHAVANREAIAVQYWQHHWPSD
                     AAGGWAPLDDPFHGKWVTAKADWWAGDRDVSFAFVPYNEEQAGEKAPDMRYRRTYRLR
                     FLCGKNELPPVRYLRAYGPNKTVEATFDIEMDGTSRLDAPLAVSVVNGYVLAEDKKTT
                     MTTARLATSPANMTLRFFEGDVESKTRTIVTVRSSRDSMEGFSFLPAEAVKYGVIRIP
                     SLGVVVSHRGSQADVEAGLKGGMAVFDRVAAEPEQTWERARREIPVLSKVKHEGGPLY
                     LPLGPPEARQEIAVRYDGSFLLDSSALKSGGVDAERMQWPANRWLIRLISGEPAFDHE
                     TEGNVKQQLLDGYLPIVVNSWQANGIDYRQTSVAGFLDEKHGNVQGDETVVLMSRLEM
                     TNSGQSDAEAVVKLWNEPHEAMVLENGNILAKGRYEDGKLRNYENPRIRFRVNAPDGS
                     LKIGPESEQMPDAVLTYRRKLAAGASEAVVLAIPFVTIDTDAELQQLAGLGFDRMLEH
                     EVVRWRRIISNAAKVEVPNQLLNDFYKAQLSHILITADRDVHNGLMLAPAATFSYNVC
                     LNESCHQIRALEIRGLHDEARRFLDFAIEGQSSRGLHGRFTDRKGVLHGMPTKDGDYQ
                     HFNYNLDHGFVLWMLNEHYRYTRDQEWLGKVANAMVQACDFVTRQRKTGPEANTLGRD
                     DDLWGKGLLPPGHLEDPPEWLWWFAVNAYAYRGMKLTAESLAEIKHPEAERIAKDAED
                     FGRCLRDSCRESMLRAPVVRLRDGTYIPHQPTRSRLRGRDLGWIRDALYGPVHLMDCF
                     VYPDDSREAEWILRDTEDNVFIGAARGRAVTDYEQQWFSWGGITLQSNLLPNPLIYLR
                     RGESKHAIRAFFNSLAANVYEDVRTFCEHPIKRYGVGTGPFFKSPDESAFIVWLRHLL
                     ILEQGDGLHLLAGVPQAWLAPGKEIVVERAASWYGPIDMKVTSQDTPRQIRVEIAGPS
                     RSPGTIRLHARVGQPLKSVTVNDKPTKDFDAEKGIVMLRTSGGRTIVVISY"
     gene            complement(19014..19202)
                     /locus_tag="P5316_01955"
     CDS             complement(19014..19202)
                     /locus_tag="P5316_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRR83750.1"
                     /translation="MRLGGARQYAAGCRRFFAESEAATVTEYAVMLAFLISVMVGGIT
                     LLGAQVNNLFTSAVNAGW"
     gene            19975..21303
                     /locus_tag="P5316_01960"
     CDS             19975..21303
                     /locus_tag="P5316_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83751.1"
                     /translation="MRSCPGSLRRAGALKVLLIIFFVMVLVCAGLVTLAIMNGRSLLA
                     WMISKPLKALVQQCSLPDDQKTRLTASLDGLIADFQNERVSIAQLSGIFQELAEEPFI
                     DLVLIEIVRNEAVKSGHFSDQDLGDLSLSLDRLQRGIIEGILDSDAVKATMDCVSTAQ
                     PDGARQLKQPLMPADIKRLVAEATKQADRAGIPRERYLADIAGEVKRVMDRQLGKGTS
                     TAPAAVPAPPPPQPVGVPEPPPPPASPAFSTAPNAMTAIPVAVPVPVAPPPAAVVSEP
                     PAAVSPASAPVSPATQPVATSAQPSPAQPAEPQPPASAEPPKLVEPQTVPSTPPASQA
                     EQTTSMPAGVVESRPSAATAEPAESPVTGPAPAPQPPSTASASIQPSSQPAAGPPPPA
                     ASAAQPLPAPTTGPAIPPAESAPAMATQPLSAAAALPTVPAPVPHTLPGP"
     gene            21379..22245
                     /gene="mutM"
                     /locus_tag="P5316_01965"
     CDS             21379..22245
                     /gene="mutM"
                     /locus_tag="P5316_01965"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRR83752.1"
                     /translation="MPELPEVETVCRGVGQRITGYTIEQVHLNRADVVHGAAIPLCAA
                     LHGRRIKSIERVGKQIIIVTDGAVALMVHLGMTGKLIAVHPEMPIEPHTHLRIGFRGR
                     KVELRFVDPRRFGGIWLLGADSSRVGWIGRQRPSVGPDALTVSCEEFARRLARRRQIK
                     ALLLDQQVLGGVGNIYCDESLHRAGLHPAVRADRLSIEQIRRLYRTIRSVLAQAIKAG
                     GSSISDYRNADNAPGRFQVRHRVYDREGQPCRRCKTAIRRLVVAGRGTFICPKCQPGP
                     RVGSPRPLRRQT"
     gene            22352..22981
                     /locus_tag="P5316_01970"
     CDS             22352..22981
                     /locus_tag="P5316_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83753.1"
                     /translation="MKTAKLFTIALVSMLLAVAGCQMPPQPVGPTLGTVSAETPEHVD
                     RLWESVETALLTFNFDIDRRDRVQGIMTTYPETSASGFELWRVQPTPAYYWWESNIHT
                     IQRQADVYIREAPAGGNCELEVKVDRYRLSLEERQVDNAAAAMRLYSREAPTLSGQML
                     GSSKSTRRIHLGRDGFLEQAILEAIFREYKKPTTTGPAGDSGSTVVKGG"
     gene            23132..23392
                     /locus_tag="P5316_01975"
     CDS             23132..23392
                     /locus_tag="P5316_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83754.1"
                     /translation="MLTGIGIEDVMVNRQQAEPKIWNWRVRARSIDSQIVTLGKYDTE
                     DEAKADRDKIVKEGFYRNVRVEQIKPKGGTQQPTTAENQPAK"
     gene            23949..25013
                     /locus_tag="P5316_01980"
     CDS             23949..25013
                     /locus_tag="P5316_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83755.1"
                     /translation="MKCHAEFIIGGDKPTDEDTIVAWITEDDPSSRSVMGSTGIFQGP
                     ETHQEAPKASASKPVPMSVAPEPLQQVVHLEGIRDNGAHFEFPAAALTREDLRDSFPR
                     KCVGCSGRNDLRVHLLYWPERMLGKERTYWKDRVETVVGDLERFANSAGSGLLRQLPR
                     TRHLEHPFDLPFPVFACRYCRPSEEVETHVSGRPPHDVCRLRIASLSVAVSFFRNNGG
                     RGSHAYQRLIEERDRRQDPWRALDPQIRHGILQWFQPQPGETFVRFIHDRDFSPAQAA
                     VAGSVLTDRRLVFKLQSECEGYPLNGEGRIEIMRKGDLAIAHIYLHGQRPAVLKLGIV
                     QADELLDSLRQLRCRWTVVT"
     gene            complement(25069..26238)
                     /locus_tag="P5316_01985"
     CDS             complement(25069..26238)
                     /locus_tag="P5316_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR83756.1"
                     /translation="MTYTSYERIKAALEHREPDRVPFDLGGTVLTGMNTVAYRNLRKY
                     LGLPEVEVQTYDTKQQLARIDQDVLDRLMVDVRCVDPGQAQTPLATPVRREGDYHCYQ
                     DQWGITWRMPVQDGLYFDMYKHPLADAESVADLEKYPWPDPEDPERFATMKQRADRFV
                     HEEKKAYVLGRHAAGLFEVSLWLRGFENFLVDMAANPGFAHALLDIVTDLKMRYWKKA
                     LETVGENVLIVSEADDIATQRGPIMSMAMYREFIAPRHRRLFEHIRSSAKSRVYIFFH
                     SCGAVKDLIPQLIDEGIDILNPVQVSAEGMDTRELKRLYGRDITFWGGGVDTQRVLPY
                     GTPRQVRDEVRRRIDDLAPGGGFVFNPVHNVQGDVPPENYMAMWETLCEYGVYAS"
     gene            26374..27129
                     /locus_tag="P5316_01990"
     CDS             26374..27129
                     /locus_tag="P5316_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRR83757.1"
                     /translation="MAAAQLHVGTSGWCYAHWAQGRFYPKGLRQGEWLRFYAEQFSTV
                     ELNASFYRLPKTEMAARWREITGARFLFAVKLWRRITHDKKLEDCTDELRGFLAAVEP
                     LGTKRGPLLVQLPPGMRCDAGRLDRFLEELGRQSTGHHWRVTVEFRNDTWLVDEVYGV
                     LDRHKASVCLADMGRCPITRPNDVDFVYIRRHGPAGSYRGCYSLDELRHDARQIRRWL
                     NAGRDVYVYFNNDAEANAVTNARELIDLLDGRS"
     gene            complement(27190..27651)
                     /locus_tag="P5316_01995"
     CDS             complement(27190..27651)
                     /locus_tag="P5316_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRR83758.1"
                     /translation="MLFQLLLLFTLMPLIELAVMIKVGGIIGVGPTIAMVLAAGVLGA
                     ALARHEGLRTLHGVRADLEAGRLPGDRMVDALLILVAGVLLVAPGFVTDVIALLLLIP
                     PMRALARRYLKRRFAAGFTIVNMPGPDSGRPPGPDDLIDVEAHSPQDSERS"
     gene            complement(27656..28006)
                     /locus_tag="P5316_02000"
     CDS             complement(27656..28006)
                     /locus_tag="P5316_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRR83759.1"
                     /translation="MPTDEDTTVAELRREIAAFIRDRDWEQFHDPKNLSMAIATEAAE
                     LMEHFRWIKNDPSREWVKDAGNKADVAEELADVLAFVLSFANTADIDIATSLRAKMAK
                     NAMKYPVREYKGKY"
     gene            complement(28136..30109)
                     /locus_tag="P5316_02005"
     CDS             complement(28136..30109)
                     /locus_tag="P5316_02005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRR83760.1"
                     /translation="MSQYTLQTPSVSVQRDPPNRLAAITFSILIRLAVLALVVVATLG
                     AIWGGPGLGDHEAIVAECGRNMRLSGDWIVPTFLDTPWMRKPPLPYWLVAGASYLLPN
                     DPQLDLPVTTVSARLPTAVSALLTVLLLWHLASSMFGRRVGPVTALVSGSSVAFLMYS
                     VNATAEMLLTLCCTWALVHFWHGVTTRRAWVRFVHMMLFYVALGAGMLAKGPAPMALT
                     AVPLAVWWYTERPLRLLAFLGLRGWRQVLTCFGRQIPRQTARAFTRLYLVPGLIVFAA
                     IFVPWMIAVGRQFPHAWDIWNWQYWQRAQGFYDDTRPRGAFYYVPIIIGLTFPWLFLL
                     AEAVAAPWLKRYAWQRRALFFAGFCWLIGLAVMSLLSFKKPYYILPAVPPLLLMMGLV
                     AERFYAFVPTTIPVKLKIWFGRWHDVVIQDPLRFAWIAWAIVAVSAILMLVFGNFWMN
                     AYIPKVAGALTVIAAGALVVLLWAGLLYVWGHGWWALALTSVATVAAFHSAWYICGPT
                     LAAENSERVRMLDEALDQAGVPPGASIYWTYSRPDARLSFYYGRRPRYLVTPEEVVGF
                     IGVNRTRPGVRELLEAHVLKKAENLLAGDETVYLLASYEKYKKARNEVKSVTFYDIAR
                     AKCDPGNPDKDWMVISNRPPMKAASLPASQAAP"
     gene            30165..31064
                     /locus_tag="P5316_02010"
     CDS             30165..31064
                     /locus_tag="P5316_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRR83761.1"
                     /translation="MELRNYREERRRSRREEQALDNSPLVSIVVPTYREEANLRPLVT
                     RIAAAMESADHECEILVVDDDSRDGTDIAVQELAERYPVRLITRTGPRDLSLAVLEGL
                     RQARGQILLVMDADLSHPPEQIPELLKALNQPPTDFVIGSRYMPGGHTEEGWGVFRRL
                     NSLAATLLCRPLVGRVTDPMAGFFALRRDTLVNADPLDPIGYKIGLELICRCRCRHVR
                     EVPICFANRTCGQSKLNLDQQSRYLVHLGRLYRSYSRGWGLVIRPILAVMRAGIAVVE
                     YLRSSRPDVVASISVAVHKNRAA"
     gene            31557..33248
                     /locus_tag="P5316_02015"
     CDS             31557..33248
                     /locus_tag="P5316_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83762.1"
                     /translation="MQKLSITETPNELTTDIDVASPVGIVRMLRAADAQIYSGYRDYP
                     AVCDAEIVAKIVRTIEWAAPVLGREDSVVVISGAGTSGRLAMFAARTFNRFLAGCKRA
                     PNVRYLIAGGNLALIKAQEGAEDDPHKAVDDVEPLVRGKRRVLYIGVTCGLSAPYVAS
                     QMLHLSRRRGTKCVLIGFSPADRARDVEVEDWDKTFASALQSVLRRRNFLLLNPVVGP
                     EPITGSTRMKSGSATKLLLEVIFALAVEQGGQGVLPERVAACLKAYEQTRVAVYEQTA
                     AIGRLVELGGRALRQGGHIYYLGADTPGILGTVDASECPPTFGADFDTVRGFVVGGWR
                     TLLGPGHDLSDEGPWYRISLDEFKQVKLPALSGKDLVVGLGMDLHRQVGEALHRSRNA
                     GARTAVVQVGRRARGRVAVDAAVCVPNVPKTIIPGAKVFEEFGTKLILNALTTGGHVL
                     SGKVYQNRMVDLRISNNKLFYRTIGIIQHISGVSAEAAKRHLLRSIYGTDRVTPRMMN
                     AKPSQHVQASTNVTKIVPKALIMAMGRTTPAEADRLLKKEPIVRAAIERLKSAST"
     gene            complement(33584..33657)
                     /locus_tag="P5316_02020"
     tRNA            complement(33584..33657)
                     /locus_tag="P5316_02020"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33621..33623),aa:Met,seq:cat)
     gene            34181..35359
                     /gene="dgoD"
                     /locus_tag="P5316_02025"
     CDS             34181..35359
                     /gene="dgoD"
                     /locus_tag="P5316_02025"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HRR83763.1"
                     /translation="MRITAIETHVCNARMRNWVFVKVVTDQPGLWGWGEATLEWHTRA
                     VVGAIQDIEPLLIGEDPTRVEHLWQMMYRQHFWHGNGIIRATAMSGIDLALWDIVGKI
                     HNVPCHKLWGGPVRDYIRTYCHLGGGKMEDFYETDPKDATRFAELARKAVEDGFTAFK
                     TMAVPWTMPIEGLRPIKYAEACVRAMREAVGDDIDIMVDCHARPSPRMGVLFAKALEP
                     YGLYWFEEPCWPETVDGLAEVQRAVVTPIATGERLISQHGFRELLEKKACSIIQPDIT
                     HVGGLSEARRVAAMAEVYRVAMAPHNPQGPVSTAASLEFGFATPSYIICEAVHLDVPW
                     RQDVVREGFTVEKKGRIVRPGSKPGLGIEIDEAEVKKHPFQQELPQRVFYSDGSVGDW
                     "
     gene            complement(35366..36352)
                     /locus_tag="P5316_02030"
     CDS             complement(35366..36352)
                     /locus_tag="P5316_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR83764.1"
                     /translation="MLLYSPFTSLQNSKRPAGFSLIEVLVVVAMVALLMAILLPSLSG
                     ARELARAAMCGSSLKQISLAATAYGLEQRGWLVGSPNTSGNGAPPGFAAGAYTPTASR
                     DHYPALQIFDWASPLLKEFGTRAPRDFKQRYTAAVSAVPQCPTNRRRTSPVNYPELTD
                     LIPADTLAPSYAMSRYFTYVGDKAKSGETAGTLWWHEDCIPARYVPKPDLIKQPAGKA
                     FLADAHIVSKTKGEIANANWGFSSQGAWRSHDEGPVTYRGDFLRKEMWRHRGTINIAA
                     FDGHVERQREGSSDANNGKGSEARRAKWWFPSGTKTELLPSKATCEPEIIVP"
     gene            complement(36610..>39642)
                     /locus_tag="P5316_02035"
     CDS             complement(36610..>39642)
                     /locus_tag="P5316_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRR83765.1"
                     /translation="VFPEFDLDMVMVTVPYPGASPEEVEQGIILAIEEGIRGITGVKE
                     VTAVAREGSGMVTAELEENTDAQRVYQEIRQEVDRITTFPEDAEEPDVVLMTHRREVM
                     DVILYGDADEWILRELAEQVRDRLLQDPNVTQVDLEPARTFEVSIEVPQENLRAYNLT
                     LQAIAERIRSASIELPGGSLRTDSGEILLRMKERRDWAREFENIPIITSADGTQLLVR
                     DLATVRDTFEEEYRFVTYNGKTAVNLEVYRVGKQTPIGVSKAVWKKLEEIRPALPPGV
                     YIDVRRDESEIYRQRLDLLLRNGAMGLALVLVFLTIFLEYKLAFWVAMGIPISFLGSF
                     LLLPAMDVTINMISMFAFIIALGIVVDDAIVVGENVFDYRQSGMGRLEAAIRGAKEVG
                     VPVTFSILTNIAAFAPLAFMPGTMGKIWRVIPLVVSSVFTISLLESMFVLPSHLAHTK
                     KENATSLGQVIHRAQDRVSKLISRMIEKGFGPLLAAVLRFRYLTVSLGAGILVLALGY
                     VASGRMGFEMMPKVEADEADATAILPYGSPLSKLQEVSDRLVRSAQSVAGENGGERLM
                     RGVRAYIRENEIFVTAYLLAPEIRPISTAEFARLWREKTGVIEGLQSLRFESDRRGPG
                     SGASMTVELAHRDIEVLDRASEELAASLAHFANVRDIDDGYTPGKQQLNFRMLPEGRS
                     LGLTAAAVARQLRGAFYGAEAIRQQRGRNEVKVMVRLPKAQRISGFDVESFIVRTPAG
                     ADVPLHQVATVQAGRAYTAINRRDGRRTVSVTANVVPQSETDRVLEMLTETTLPDLMS
                     KYPGLTYSFEGRQQDMRESLASLQSGFVIALMLVYVLLGIPFRSYLQPAIVMISIPFG
                     IVGALLGHMIMGYSLSVISMMGLVALSGVVVNDALVLVEYANRQRTKGLSAHEAILSA
                     GVRRFRPIILTTVTTFGGLAPMIFETSRQARFMIPMALSLGYGILFATAITLLIIPCL
                     YLILNDAQRLIGWSTPASDEESTQKLPEIALQRADASR"
BASE COUNT         7812 a        11876 c        12548 g         7406 t
ORIGIN      
        1 tgaccgagct gtagcgcatg ttgcccatgt aaggcgccgt cccgaccgtt ccgccgatga
       61 tgttgctgag gttggccgaa ttaaccgtcc cgccggcgga gtcgatctcg gcgacctgga
      121 cgtcattgtc gaccaccttg gcaacgaaca accgagcatc atcgccaccc gttccggcga
      181 aggtgagcgt ttcccagttg gtgccgtccg gcaacaggtc agcaacctgc gtgccgtcaa
      241 gctcgcgata cactctgagt tggccgcctt gggcgatatc gcgcgtgacg tacacatagt
      301 ctgtttcgaa tccgttgttc agtaccgcgc cggcctcctg gattcgtccc agtatcacgg
      361 ccgacatgct caatatgatc gccaactcgc ttcggcgtct catggctcga cgctcctttc
      421 atggaagttc acgctcgcgc actcgctgcg cagccacctt tggaaatggc cgagacagtg
      481 ccaggaacat ctcctcgtac acggaccgat ggctcaccgc ctgcgccgca aaccgagcag
      541 gcccagcccc aacaggatca acgatgccgg ctcgggcgcg gggctatccg cgtccagcca
      601 atagcgactg ccctcccagt aggaggtcgt atcatcggga ttgagcccca tgatgccgcc
      661 gcgaaaagcg ccgaaccaga ccgtcccgct cacctcgtcc aactgcccgt tccaaccgcg
      721 cttcgcccag ataggttgcc cgcgattgac caagttgccg ttgctgtcga tctcgtcgag
      781 atagaactcc agggtaggtt cccagccaga ggtctggttt gaagggataa gaatggttgg
      841 ccggccgctg ggattgttcg tgccccggaa cattacgttg ttgggctggc tgtactcggc
      901 atctccgacg gcgtactcgg ccccatcgat cagcgtcgag taaacgttgg tcgtgccgcc
      961 gagcactcgc ccgatggtgt taaatgagat caggttgccc aagccgtcgg ccgttgggcg
     1021 tcccaaatgc cgactgatca tgtatagcga gccgtccctc tcgttgaagt caacgttgac
     1081 cgggcgctcc tcacctgcgg agtttggcag tgtggcgccc acatacgtgt gctggagttg
     1141 gctcagccca aggtcaatct cccaggcctt ggcattaacg ccgatcacag tggtatccgg
     1201 gttggcgccc acaatcagcg agttgtgcat gcggttatag cggatgttgc ccagccgcag
     1261 gcctgacccc agcgtcacgc ccggaatact gctcagactg accgatttga ccgtctggcc
     1321 gagcgcgttg acctctgcga tctggatgtc cgttccaggg tcgccgccgg tcatcttggc
     1381 cacgaacagc cgggcatcgt tggttcctga accagcaaac gtcagcgtgg tccaattgga
     1441 gccgtccggc agcagattgc ccatattggc cccgtcgctt tctctgtaca tccgcgtctg
     1501 accgccctgt ccggtatccc gcaggacata cacgtagtcg gtttcgaagc cgttgttgag
     1561 gtacccccag acgttcgggg ccaaggacgt gattacgccg agtgcgacca caactgtggc
     1621 cagttctcgt attttcatcg ctttctcctc gctttcgttg agatgaagta ccttggttta
     1681 cctacgacta tataccagga ggcatgttca ggtgatcgcc ctcctctttg tgcggacagc
     1741 ctgcgagccc gatcggcgtc tcctttcggg ccgggggctt ggacttgacc gaaaggctgg
     1801 ccgaccggct tgatcatcgg cggcgaaatg tgcgacccca gggcctttgc tgaggagtgc
     1861 cgttcatcga gatgccgaat gacactccca accggtaagc ccgggagccg gagttcaccg
     1921 acgcttatga gtgtactggg cgtcgggccg gcgtgcaagg aaaaacatgg ggcgctggca
     1981 ttctgatgcc gtgccgtcgc acttgcgtcc ggatacataa gtgtttctcg caaaagagct
     2041 tggcgtggag tcggcgagct ggaagaaaca gcatcatcgc ccgttgtgaa gtgccggcgg
     2101 ggtcgctgaa cccggcttcg gccatgagaa ctcttgttac cttggggacg ggttgggatt
     2161 tccgatcgcg agagcccagt ctgagttgtg gggggccatg aaaacctggt caggcgccgc
     2221 cttcagtccg gtcaggggaa tctcgcggta cacggccttg gtgtccaggg ccaccgcctc
     2281 cagaggctcg gtcaacccgg cgaggttcat gcggatcgcg tcggcgtcct ccttgtagaa
     2341 cacgcagaag cggtcagtta tctcaagaca cacgccgtcg gtccgggcgt tgtttcgtac
     2401 catctgctta tgaaaacggc tcttccagaa gcgcgagtag gtgcggatct gttccttgtt
     2461 tggatagacc gccgaacttc catccctggg cgggttgacc aggtaggccc agatgttggc
     2521 cacgcccccg gccattgttg aattccacag gcctcggcgg gtcatgtcca gatcgtagtc
     2581 cttttccgga taaacgttga cgcgaacgcg gaagcggtcc tccatgaagg acggcttgcc
     2641 gggagagcgt ttctccatgg cttccacgta ggtgtcatag gtaggccgcc actgctggta
     2701 tgaagagtaa tcgaggccgt cgtagatctg ctcaaggtcc ggcgagcgac cgcccagaag
     2761 atgtggccat ccaagatgct ggtgcatgtg cttgtgccat aatcgcaggt gctcccggcc
     2821 ggcccattcc tggaggtcga atccataccc catcgaccag cccaccaagg ggcccagacg
     2881 ggcgcagatg tatcgctgaa gacggcggtc taccgaaccg ttcattcccc agcgaaccgg
     2941 agtgatcttg cgctgctcat cgccccacat ccagatgtgt accactccgc cgttcttatg
     3001 ggccttgctg atcagcagtt ccagggcttc aaaggtgcgc gggtcgggat tggggtcatc
     3061 cgagggaatg tcgcggtagt tgccttgtcc tttgttgatg tcgaaccagg ccatgcccac
     3121 aaacgtgtga aaaccgttga acccatgatc ctcgaaccag gtcctgatgt cggcgtcgat
     3181 cttgtccggc ctgttgaagt aatccgccgg cccacagtag ctcacgaatt ggggaacgaa
     3241 ggcttcgtcc agacccaggc gaccccactt gttgccgaag gcgactgtga agccggccaa
     3301 gccggggttg ggctcgacgg tcagctcgcc tttgcggtcg ttgaggtcgg ggtctttgct
     3361 cgccgtcgtg tattgccaga cacccgggcg agtgccggtg aaacggagga cccacgttcg
     3421 gccccggcgt aaaagagctc cgtttcgatt cgctcgagac tctcgttgtg tcggaatgtg
     3481 gccctggccg tcaggtggaa ggcattcccg tcccgcacag ggttctcgaa ccaccattcg
     3541 accggctccc actgtttggc ggttcttgca tgcgcggccg ccactgccag gcatccccaa
     3601 gccaagagcg tcagagtcct gcatttcatc tgcgagtcct cattgagatc cgaagggcaa
     3661 gcgtgagtcg ttcgcaccgc acgataccgc gcgagcaatg ccttttcgag ccagtctgcc
     3721 atcattgaag cggcaggttt caggggattg gcgtcgtccg ggctgttggc aggcggtttt
     3781 gtgtttgaaa ctttttacgt gtgacttatc gggccatggg gtccttcgat tgacgggacc
     3841 gatgccaaac cggtggctga gcaaagagag aagacaactg aagcggatcg ccttcttgcg
     3901 ggccgcaggc caagcgcccc gtgacccgaa aaccttcgcg caaaacggac accggccagg
     3961 aagagactga caccggcgat tcggcgcgat ataatggcgt cacgtcgcca acctattccc
     4021 tgggacgatc gtcaagagca aagcatcgag gagttgttgg ttatgtctgg acttttgcaa
     4081 agtcaggcta acgccaaatg agccttgatt ctattacatt cccagccctc ctccacgcgt
     4141 tcgatgaccc aggaaatcgt ctggctcagc gtttgtcgca gcaccgcggt gcaggcttgc
     4201 ccgatggtcg tcaggcgttc gagtgcccag tcccgcgcac ggccctgccg cagctgacgc
     4261 atcagaacgc tgtgcgccag gaagaccatg taccaatggc gggtctgccc ctggcccttc
     4321 ctgagctgac agtcgcccat gcccagatgc tgcttgccgt cccgatggaa gcactcggtg
     4381 cctcgccagc ggcaccggta aacgcggaga atacggctga cctcccaata ggttcggttg
     4441 ctgaccagga ttttgcgggg gtgggcgccg ttccgccttt cccacaagat cacgatccga
     4501 accggatgat cgacctgggg gatacggatg ctcttggtga aataccactg ccggtggtca
     4561 ccgatatcca ccggcttgcg atcctccgga ccgatgaggg ccgccaccgc ttcggccttg
     4621 atcgtttgtc ctcggaaaac gaccttgcgg ttgaacttga ggtcgcccac gtagccccgg
     4681 cgtcggcggt gaatatggtt caggttggcg gcatgcgtga accagttgtc gaacgtgaaa
     4741 tcgcccggaa taccgtgccg gatgacctcg tccaccagtt cccggaagaa cacgttgtgg
     4801 tcgcggaacg ggaggccctg cttctcacac tgttcccgct tgacgaagcg acggaactcg
     4861 agggggtaat gcttgccgct ggtgcacaca taattggcga tgaggtaatc gtgggcgatc
     4921 ttgtaccggt cctccgcgtg gtcccagaag tacccgacgt cctcgatcag tttgccgtca
     4981 tggtcgacca gaacgttatc cagggcaatg accccttgcg ggctgtaacg ggtggagggg
     5041 tctttctgaa gccatttgag ccgctgctga ttcagccggt ggacgtccca gtccacctcg
     5101 gtcagccacc ggttcagaca ggactgatcg gtggtgacca cgaactggcg gttgatggcc
     5161 gaaacgttct tgcggtcggc caccagcagg ccggtcaggt actcggcgaa atgaatccgt
     5221 tcaggctcgt tggcgaacag atgaccgaac tcgttcagag cctccttgac gatcgtaggg
     5281 aagtcgacga tggccggcat ggcggtatcc tccatgaccc gaaaaggcgt cctgccgtca
     5341 atggtcatcg tcaaaatgcc cataaatcac caacgaaatg ctactttgca aaagctcagt
     5401 tatgtgtttc aaagggttga tcggaaagca gtcctttcgc ttcggcagtc ttatttcgtt
     5461 cacagcggcg gtgacgttga ccgtcaccac gtttctttgc ctgaccggtt gccgtccggg
     5521 tgatggcaat ccatcggacg gcacctacac gatcacggtt acggtgaacc ccgccgaagg
     5581 aggcaccgtc acgattgatc ctgctgaagg gccttacacg gcgggacagg tcgtcactct
     5641 cacagcccaa gcatcggccg gctacgagtt tgaccgctgg cagggaagca tctccagcac
     5701 ggcgaacccc gcgcaggtta ccgtggacga caacctggtg attgaagccg ttttcgtcgc
     5761 caccggtaac ggaggcgggg gaggcggcgc gacggatacg gagatcaatg agaacgtgcg
     5821 ggtactgccg gacaccgttg aagtcaccac ggacgacgaa gggcatacca cactgaccgg
     5881 ccaaggcatt cccaccggaa tcgcgccagg ggcgttgctc gtctccgccc gcggggaggg
     5941 acggttgctt cgcgtcaagt cggtgccaac agtcggcgac ggccaggtga tcgttgaaac
     6001 cgaacaggcc tcgctcgccg atctgatccg caaaggcagt ctcgaaatca gcgttccctt
     6061 cgatccgaac accgcggtgc ttcaaggttt ctcgggcgtc ggttccgggg acatgaaaca
     6121 cgttcgtttt cttcgtaccg gcgatcgctc ggccgctttt acgaccaaca gccactccat
     6181 cgaactggag gactggggca aggtcactct cgaccagttg acgatcaacg tcgatcccga
     6241 cttcagcttc accgccagca tcgaggacaa cagtctcaag catctgcgat gcacggcagg
     6301 caccggcttc cacctcagca tggacgtctc gatagaagct tacaagatct cggagctctt
     6361 gaagaaggag cagaccatcg gcaaagtgaa atggcccgat ccggtcggcg tgatggtgat
     6421 cgccggtgtg ccggtggtct accaggtgtt cctggaggtc aaagtcggcg ctcaggtgaa
     6481 gttcggcgac atgggcgcga tcacggccgg actggacttc gacgccgatg ccacgctcgg
     6541 cgccgaatac aacaagggcg aaggctggga cctgatcaac gagaaatcca cggctttcac
     6601 gccacacaca ccggaatggg gcatctcgcc gatcaccgcc caggtttacg tacagccgga
     6661 gttcggcatg acgttctacg gctgtgtcgg cccgtcgtta tcgtggaagg agtatgtcga
     6721 actggccgga gcttaccggt atgacgtact cggcgccgaa ctggcccgcg gcagtgaatg
     6781 cgacttcaat ctcaccttca agatcatcga cggcctgccg agctacaaat attcgcacaa
     6841 cctgttcagc acacggcagg tattgctggc acgcatgctt acggccgtcg atcccgctga
     6901 actgggcagc gccagtcttt cgcccggcga cctggtctgg gacctttacg acttcacgaa
     6961 atcgctcacg gccacggctc ggccgaacgc gggttacgaa gtggcgggtt ggaccattta
     7021 caatctgctc agcggcagcg gtgaaaccgt gcacgcgagc caattgcaca accagccggt
     7081 caacgcctcc aagctgtttg tggccggcat cgtgccggaa ggctacggcg acacctggac
     7141 cggcgccggc accggatcgg gcgcacccag ctacgcgctg acgaccgagg tgtttcctcc
     7201 ggaggccggt gagatcatgc tcttcccggg cggcggcgtg taccagtacg gtgtcaacgt
     7261 tctcgtagcc ccgaaaccga acagtgggtt cgagttccat cattgggaag gggccctcag
     7321 cggcaacggc ccgaccggcg tgatcagcat gacctccagc aggaccgttc gcgccgtttt
     7381 cgcgtccaat ccgccgcgca cgctgtacgt accgagtcat taccccacgc tgcaggatgc
     7441 cctcaatgcg gccacttaca acgacatcat ccacatcgag gccggcaccc acgccggcac
     7501 cggtttccat gacatcagta ttcccaggga ccacatcgtg attcagggcg ccgggccgtc
     7561 tgcaagcatc atcgacctcg aaggcgtcgc tcagctcggt tggctggacg acatacacat
     7621 cacctttcag aacctgcaaa tctcccgcgg cctgggggat ctgggaggcg ccctccgtct
     7681 gaccggagac accaccgcct cgttcaagaa ctgtaccttc agctcgtgca acgccagcta
     7741 cggcggtgcc gtttacgtct cgggcaccgg ggccatcgtc tcgttcgaga attgccgatt
     7801 cgagtccaat caggcgacct cctcgggcgg ggccctcagt ctgtcgaaca cccccaacga
     7861 cgaggagcac gtgccggtcg tggcgggctg cacattcacc ggcaacatgg cggattcttc
     7921 gggagggggt atcgtcgcgg ggcagaagtg tcggatcacc ggttgcactt ttgcgcagaa
     7981 ccgggcctgg cggggcgggg gtggttcgtt cggcaaggag ggcgttgtca gtggttgcgt
     8041 gtttgacggc aacagtgccc aatacggcgg cggcgtttct gcagacgagt attcctcgtt
     8101 cagtgaatgc tctttcatca ataatgtcgc cgagtggagc ggtggcggcg tcaaggccgg
     8161 caatggcgtg tctattcagt attgcacgct ggacaacaat caggccggcg aggacggcgg
     8221 ggccatttac gccgactgcg tttcggccgg caagactctc ttcatcgtcg gctgcgcagt
     8281 cagcgggaac tccgccggcg aaaacggcgg gggcatctgg tgcgacggca cggaagtttc
     8341 ccagacagag ctgaccggca acagcgccgg ctggcgcggg ggcggcatct gcgccgatca
     8401 cagccagatt tcttcctgct tcctttacca gaaccaggcc ggcgccgccg ggaatccctc
     8461 ttacagcgaa ggcgggggca tctatctgga acaaacgaac gtctccaact gccaactgat
     8521 tgacaacgag gccattgacg gcaatggcgg cggcatctac gcccagggca gcttttcaga
     8581 cacggccgtc ccgccggtca tcttcgaggt ttacgccgct ttcaacaccg ccaagaacgg
     8641 cggcggcatg gggagtgact tggaggtgca cattttcagc tcgatcttcg aggacaatga
     8701 ggcatcctat cgaggcggcg gcatttatgc tgaacgcggt cccctcgtga gcctctgccg
     8761 ggtcgagcac aactcctcgc agcagggcgg cggagccggc atatcggaag ggaccatcct
     8821 ggcttcgacg atcacccgaa atcaggcctc gcaatgggcg ggcggactca gtttgttcaa
     8881 gagtacggcc caggactgca ccgtcagtca caataccgca gccacagcgg gcggcggcat
     8941 ctggacgggc atcgagtgct cgatctccaa ctgcaccgtg gcctataacg ccctcaccga
     9001 cacgacctcg acgggcggcg gcggcatagc ttgccacgga gcaccaacgc tgagcggcgt
     9061 gactgtttac ggcaacacgc cgaccaactg caacagtgac tgcccttgcc cgtgacgggc
     9121 cgcaagcatg ggcttgcaga gcgggaatct acgaatcgag gctgaactga aggtctgcaa
     9181 gctgcggttg caggaccgtg tgcagttggc cggggaccgg caagcgtcga tgctgggcgt
     9241 cgagcgtcaa gagcgtgatg caacttgtcc ggaggcggac cgcctttttg cgggccgcag
     9301 gccaagcggg cgtaaaccgg caatatgcgc gcgagccgca cagatgtccg gccgtaagga
     9361 ttgaaaggcg catgcccacg caaagccgtg ggcatggcac ccggcgatcg ggagacatgc
     9421 gcgcaaaacg cgcaccagtc gcgacggaag agactaaacg gtcacgggcc cgcgggaacc
     9481 tttcctacgc catgcccacg gtcagcagca gtgggaacca gtagttgagt ttgcggcggc
     9541 ggatttcgcg gaagcctgcg gttttgaagt aggcgtcgat caccgaccag ggcgcgtggt
     9601 ggacgtgttt ttcgaccgcg gcgatgatca cgtcgtaggt gacccagccg acgatgttgt
     9661 cgcggaatcc gtcgataagc atgaacctgc cgcccggtcg caggatgcgt cggacttcgg
     9721 tgacgacatt ctgctggtgc ggatagtggt gaaaggagtt gccgcaggtg acgacgtcga
     9781 aggaaccgtc cgcgaaaggc agatgctcgc tgtcagcggt gacgaaccgg atccgatcct
     9841 gcaggccggt tggtcgggcc ttgtcggcgg ccacctggca catggccgca gagtagtcca
     9901 gtcccacgat ccgggccggc agtgcggttg cggccagcat tccggcaaag gttcccgtcc
     9961 cgcagccgac gtccagcaga tggaacggct cgggggtgtc ttttcgccag aggtagagtt
    10021 cttcgagaaa agccagatag gacggcctga acatgaaccg gttcagcagc gaacgatcgt
    10081 agctgccggc ccaggtgtca aactcgcgtc tggcttgcga ccgcatggtc tcggctttgc
    10141 tcatgacaag agcttagtca ggtcggaaac ctctggcaaa ccggttgttg cgtcgcgcgg
    10201 gcactgagcg gcgcaccggc ctgcgatctg ccgcgagcgt tgacacctgc tgtctccggg
    10261 acgacaattc cgaccagttc ggtgcaaggg agcctgcaaa caagatgccc aaacgcaacc
    10321 tcgtctggat cgcggtcggt gctgttctcg ccgttctgct gtggaaagtg cccgaggccc
    10381 tgatccgccg ggacgcactc tacgaccagt tcggcccgct gaccgacgtc agccttcaga
    10441 tccacaggaa ttacgttgag gaagtggaca agaagaccct gctgaggggg gcgatcgacg
    10501 ggatgatgca gcggctcgat ccctacagcc gctattttga ctccgccgag tacgagcagt
    10561 tcagcaagcg gattgaaggg cagttttacg ggatcggcgt ggagatcgat cagcttcaca
    10621 ccggcgagct ggtgattgtc agtccgatcg agggcagccc cgcgtttctg gcggacctgc
    10681 gagccgacga ccggatcatc atgatcgacg acacgagaac ggtggacatt tcggccgaga
    10741 aggcggtcga actgatcaag ggggagccca acacgtcggt tcggcttacg ctgttccggc
    10801 cgtcgacggg gcagcagttc gagaagacga tcgagcgaaa gctgatcacc gtgccgacgg
    10861 tccgcggttg ggcccacacc gaggactggg aatgggatta cctgatcgat cccgagtacc
    10921 gtatcggcta tgtccgcatt ctcaacttcg agttgaacac ggccaagcag cttgacgtcg
    10981 tcgtccggaa gctgctggtc tacgatgaga tacagggtct ggtgatcgac gtccgggaca
    11041 accccggcgg gctgctggac gtcgtggtgc cggtcgccga ccggtttctc gacgaaggaa
    11101 tcatcgtcag cacccgcggc cgcaacacgc cagagcagcg acgggaggcc tctcgccagg
    11161 acacttatcc gtcgtatttg aaagtggccc tgctggtcaa cggggggtcg gccagcgctt
    11221 cggagatact cgccggtgct ctcaaggacc acaagcgggc ggttgtcgtg ggcgagaaga
    11281 ccttcggcaa ggggagcgtt cagaccctgt tcgaggtcga gaacggccac ggtatcgtga
    11341 agctgaccac tggctactgg tatttgccaa gcggcgagcg gattcatgac aaggggatca
    11401 tgccggacaa ggtcgtcgag ctgacgccgg gcgagagagc agcgatgatc gagtctcgcg
    11461 tgcgggtcta ttcgacgagg cgtccgagtc ttccggcgac ggcgacggca ccggccaccg
    11521 cttcggcccc ggcatcaagg ccctctgcaa cgtcgaccgc cacggcaccg gcgaagatca
    11581 ccgtgatgat cgatcgtcag cttcagacgg cgctggacat tcttcgcgag caaatcggtc
    11641 cggcagcgac tgccccggca agatgatagg gcgaccgcga atggaaaaag acccgcaaca
    11701 agttgcacgg cttgccgaaa aacggcggga tgagaactgg cggttccgca gtttcctgaa
    11761 gatgctcagc cggcgtcgcc agcgggaggt caaccgcatc gccgagcagt atggacttga
    11821 ggccgaatcg cggatcgact gccgagaatg cggagcctgc tgccgcgaca atgcgatacc
    11881 ggtcagcgaa gaggagattg agaggctggc cgggcgactg ggtctctcgc ccgacgaggt
    11941 acgggcgaaa tacatctctc atgacgaggc cgaaggcgac cacatcgacg cccggccgtg
    12001 cccttttctg aacggcaacc tttgttcggt gtacgacagc cggccggagg gctgccgcgg
    12061 ctacccctat atcggcggcg acatcgccac ccgcacgatc ggcatcattg agcgagccgg
    12121 aacctgtccg attatctttg acatgcttga gcgcctcaaa gacgccgtcg gattccggcg
    12181 ttaccggtga gccaagtagt gctcgatatc ctgtcggact tccggcacca ggtagcggtc
    12241 ggcaagttct ggcattgcgg ccatagcggc gacggttcgg tcatagcggg ttgcgacgtc
    12301 ggcggtggtg tcaacgaaga ggatcgtcct gcccttgagg cgacacagac ctccgccgtc
    12361 gccgcccatc ggctcggcgc ggacatccac cccaatttct tccgccaggg taagcaacga
    12421 ctcgaggcga cttgccagat ccatccgaca ccttcaagtc aattggaaag gcttgccgat
    12481 aacatactta accctcgtgc gtgagggcga ggaacgcatg gctttacagc ctcagaaaat
    12541 ccctgttcgt actccgccgg ccagggaggg ggtgaaaccg cctccacggc ccgcaacacc
    12601 ggccgcggct ccgcgtcctg cgtccgaagt cagcacttta gccaaggatg cctatgcgtt
    12661 acaagcccag gtcgacgagt tggcccggca attcgaggtc atgcaggccc aactccgcca
    12721 gacccagaag ctggccagcc tgggcaccac ggcggcgatg atcgcccacg agttcaacaa
    12781 tctgttcacg ccggtggtag cctacgccca gcaggctctg gacacgggtg acgtggaact
    12841 gatgaaaacg gccttggcca agacccttga tcggaccaat gccatgcggc agatggccga
    12901 ccgggtgatc ggcctggccc gccagccgtc ggggagtatc cggtcggtca atgtccggca
    12961 gttggccgat aacgccgtgg gctgcctgtg ccgcgacctt ggcaaggaca acatcagcct
    13021 gaacatccag atcgattccg gcttgtgcgt gcgggcagat gaaaaccagt tgctgcaggt
    13081 gatgttcaac ctcgtggtca atgctcggca agcgatgctt gggcggcggg ggcgattgac
    13141 tatcgacgcc gtgccgatcg acgaaaaccg tgtggagatc aacgtccggg acaccggctg
    13201 cggcatctct ccggagaatc ttggtcgcgt ttttgagcct ttcttctcga ccaagcagga
    13261 agccgacaaa ccggaccgcc gcgggctggg cctgggcctg gccatctccc gcaacatcat
    13321 tgaagacctg ggcggcacca tctcggtgac cagcgaagtc aacgtcggaa cgacgttcac
    13381 cgtcaccttg ccacgggcag aatgaccgag gcagaccggt ttccgccgtc tgccgacctc
    13441 tctgacaaaa gaccggctga caaacagccg gcggcatcct cgatgagacc gtcgcggccc
    13501 cgttgctgtc ggctcttgcg cgcacccctg ttgccgtgtt gggacgcgcg gtcgctggtg
    13561 gaggcggacg ccgggggtga ttatgctatc cgagcagaga gccgcgaggc cgcgcatggt
    13621 cgtgtttgtt gtggagaggc ggaaagcgtg cgtctggcgg gcggaaggta tcgggatagt
    13681 tgacgctccg accgggttcg cctggcagac ggttatcgag cggcgggttg ggcgcgtcga
    13741 cgcatgcggg cgagtggtcg atttcgggtg acagtcatga gcgcagaagc aaagggcaag
    13801 aaggggagaa ggggaagaaa gcaagcgtcc acgaaaaggt ccgccaagtc ggggcgtggc
    13861 ccgactgccg aaagtctctc aactcgacgc aagcggatga tgaggattct ggcggggttg
    13921 aaggcggcct atccggatgc cgactgcgcg ctcaagcacg gcagcgcctt ggagctgctg
    13981 gtggcaacga ttctgtcggc tcaatcaacc gatgagatgg tcaacaaggt gacgccggtg
    14041 ctgttcgccg cgtatcccaa cgcggcagcc ctggcatcgg cggacccggc ggacgtcgag
    14101 cggatcgttc atcgcacagg attttttcgc cagaagacca agagcatcat caacgcctgc
    14161 aggatgatcg tggagcgttt cgggggtgag gtgccgcaga cgatggacga attgacgcag
    14221 cttcccgggg tagcccgcaa gacagccaac gtgcttctgg gcacgtggtt tcacaagaac
    14281 gaaggggtgg tggtggacac ccatgtgggg cggctggctc accggcttgg cctgacctgg
    14341 accagcaagg acgagaagga tgcggtcaag atcgagcagg acctcatgca gatcattccc
    14401 cgcgaggagt ggacctacgt cggccacgcc ctgatccaac acggtcgccg tgtctgttca
    14461 gcgagaaagc ccaactgcgt cggctgcaat ctcaacaaac actgtccctc ggcgttcgcg
    14521 tttggcgagg agtagcaccg acacacccgt cttcactttg cggacggatc gattcgagat
    14581 accgacacga aggccggaga gccgacgagt taccactgga atgtatcggc cacttcggcg
    14641 aggtagtcgg cgctatcggc ctgatcgcgg cggagttcgc ggagcacgcc gtctctggcg
    14701 cggcgagctt cgacggcctt ttcggctgcg gccatcaact cgcagacggc cttggcatca
    14761 ggggcccgct tgagctttgt tcgaacggcc gggctgtcca gcacctgccc gctgttggcc
    14821 aggatgcgga gttgctccgg gatgtgttgc gacggaccgg caatcatcat aacgatatcg
    14881 accgcgtgcc cctggttatc ccagcggacg ggttttttga gaatcatcat ggcgatgccg
    14941 aatcggtgaa gctcggccaa gcggcagtga gggatggcgg cgccgttggt gaacgcggtg
    15001 gttcgggccc gttcgcgtgc gatcaccgcc tcctcaattg tctggcgatc ctggccaagc
    15061 tgtccggcca gcttggccgc cagtttcttg accacctcga tggtgctgcc ggcagtctcg
    15121 cagaggacta cgctttcggg tgacagactt tctgcaatca acacttcttg aaactcccga
    15181 cacgccctgt tttcggagtc gtgtcaatag cgacctcccg ccaagctgcg aacgatgtcg
    15241 atgcggacat cgcgtccatg tgcaattcct ggatctcgct gaccggaaac aaacggcatg
    15301 ccccggtact gccccggttc ccaggtgttg gcaggatgac gagattatac cccttccacc
    15361 tacccgagta caggcccgtg accgggcaac aggaggcggg ggaagagccg caagtcttga
    15421 cgagaacggc actttccgtc cgggactgag gggcttgacc gcgcgtgggg cggcgcggat
    15481 gatccgagtt gaacgcaggg gacgaccggt gccggatgtg aggtggggct gcacagggac
    15541 cgggcggccg gcagtccaag gtcagggaat ccgtaacgag gagttgtcgg gaatagcagg
    15601 tggccgatga acgcgatgct tgtgctcacg atgatggcgg gaactgcggt ggttgataag
    15661 cgcattgatc ttggagagtt tgcgagggcg gttgaataca cgggcgacaa gccggagacg
    15721 ctcgaggttc gcgaactcga ccgcggtatt gacggctgga cggcatggaa aggcgaggac
    15781 ggccagtaca tgatcggcct ggagtgggat gaaccccgcg atattgccga gacgtgcatc
    15841 gaattccggc acgccgtggc taatcgagag gccattgcgg tgcagtactg gcagcatcac
    15901 tggcccagtg acgccgccgg cggctgggct ccgcttgatg atccgtttca cggcaagtgg
    15961 gtcacggcaa aggccgactg gtgggcgggg gatcgggacg tctcgtttgc gttcgttccg
    16021 tataacgagg agcaagccgg cgagaaagct cctgacatgc gttatcgccg gacataccgt
    16081 ttgaggtttc tttgcggcaa gaacgagctg ccgccggtgc ggtatctgcg ggcatacggt
    16141 cccaacaaga cggtcgaggc tacgtttgat attgagatgg acggcaccag caggttggat
    16201 gcgcctttgg ccgtcagcgt ggtcaacggc tacgtcctgg ccgaggataa gaagacgacc
    16261 atgacgacgg ctcgcctagc gacgtcgccg gccaacatga cgctccgttt tttcgagggg
    16321 gacgttgaga gcaagactcg caccatcgtg acggttcgga gttcgcgcga ttcgatggag
    16381 gggttctcgt tcctgccggc cgaggcggtg aagtacggcg tcatccgcat cccgtcgctg
    16441 ggcgttgtcg tctcgcaccg gggcagccag gcggatgtgg aggcaggctt gaagggaggc
    16501 atggccgtgt tcgatcgcgt ggcggccgag cccgagcaga cctgggagcg ggcccgcagg
    16561 gaaatcccgg tcttgagcaa ggtcaagcat gagggtggac cgttgtacct tccgctgggt
    16621 ccgcccgaag cccgtcagga aatcgccgtc cgctacgacg gcagttttct gctggacagc
    16681 agcgcgttga aatccggcgg cgtcgacgcc gagcgcatgc agtggccggc gaaccgctgg
    16741 ttgatccgtc tcatttccgg tgagccggcc ttcgatcatg aaaccgaggg caacgtcaag
    16801 cagcagcttc tggacggcta tttgccgatc gtggtcaata gctggcaggc caacggcatc
    16861 gattacaggc agacgtccgt tgcgggtttc ctcgacgaga agcacggcaa cgtccaaggc
    16921 gatgagacgg ttgtgctcat gtcgcggttg gagatgacca atagcgggca gagcgatgct
    16981 gaggccgtcg tgaagttgtg gaacgaacct cacgaggcga tggtcttgga gaacggaaac
    17041 atcttggcca agggccggta cgaagacggc aagctgcgca actacgagaa tcctcggatc
    17101 cggttcagag tcaatgcccc cgacggcagt ctgaagatcg gccctgagtc ggaacaaatg
    17161 cccgacgcgg tgctgaccta tcggcggaag ctggcggcgg gtgcgtcgga agcggtcgtg
    17221 ttggccattc ccttcgtgac gattgacacg gacgctgagt tgcagcaact ggccgggctg
    17281 ggatttgaca ggatgctcga gcacgaggtc gttcgctggc gtcgcatcat ctcgaacgcg
    17341 gcgaaggtcg aggtgcccaa ccagctcctc aatgatttct acaaggcgca gttgagccac
    17401 atactgatca cggcggaccg ggacgtgcac aacgggctga tgctggcgcc ggccgcgacg
    17461 ttttcgtaca acgtctgcct gaacgaatcc tgccaccaga tccgagcgct ggagatccgg
    17521 gggttgcacg acgaggcccg gcggtttctc gatttcgcca tcgaggggca gtcgagccgc
    17581 ggtctgcacg gtcgattcac cgatcgaaag ggcgtgctgc acggcatgcc gaccaaggac
    17641 ggggactacc agcatttcaa ctacaacctc gaccacgggt tcgttctctg gatgctgaac
    17701 gagcactatc gctacactcg cgatcaggaa tggttaggca aagtggcgaa tgcgatggtg
    17761 caggcgtgtg atttcgtaac gcgtcagcgt aagacgggcc ccgaggcgaa cacgctcgga
    17821 cgtgacgatg atctgtgggg caaggggctg ttgccgccgg ggcatctgga ggacccacct
    17881 gagtggttgt ggtggttcgc ggtgaacgca tacgcttatc gcgggatgaa gcttaccgcc
    17941 gagtcgctgg ccgagatcaa acacccggaa gccgagagga tcgcgaaaga cgcggaggac
    18001 ttcggccgtt gtcttcgtga cagttgtcgc gagtcgatgc ttcgggctcc ggttgtgcgt
    18061 ttgcgcgacg gcacgtatat cccgcaccaa ccgacgcgga gccgattgcg gggacgtgac
    18121 cttggctgga ttcgcgatgc gctctacgga ccggtgcacc tgatggactg cttcgtgtat
    18181 ccggacgatt ctcgcgaggc cgaatggatt ctacgcgaca ccgaggataa cgtgttcatc
    18241 ggtgcggcgc gagggcgtgc ggttaccgac tatgagcagc agtggttcag ttggggcggc
    18301 atcacgctgc agtcgaatct gctgcctaac ccgctgattt atctgcgccg cggcgagagc
    18361 aagcatgcca tccgggcgtt tttcaacagt ctggcggcca acgtatatga ggacgtccgc
    18421 actttctgcg agcatcccat caagaggtat ggcgtgggga cggggccgtt tttcaagtcg
    18481 cctgatgagt cggcctttat tgtgtggctg cggcatctgc tgattctgga acaaggtgac
    18541 ggactgcacc tgctggcggg cgtaccacag gcctggctgg cgccgggcaa ggagattgtc
    18601 gttgaaaggg ctgcgagctg gtacggtccg atcgacatga aggttacatc acaggacacg
    18661 ccgcgtcaga taagagtgga gatcgccggt ccgtcgcgat cgcctgggac aataagactg
    18721 cacgcccgcg tcggccagcc gctcaaatcg gtgacggtca acgacaagcc gacaaaggat
    18781 ttcgacgccg agaagggcat tgtgatgctg cgaacatccg gagggcgcac tatcgttgtc
    18841 atctcgtatt gagaaggctg atgggatgcg cgtgcgaaat gtcgcaaccg gccggcatct
    18901 tgcgtctgag atcacaggca agataccgat cccgcgcgat tcctgcatgc gcttggatgg
    18961 cagactaacc gtttcgggcc gcagaccggc tgttcggcaa catacggata tccctaccag
    19021 ccagcgttca cggccgacgt gaacaggttg ttgacttgtg ccccgagcag cgtgattcct
    19081 ccgaccatca ccgaaataag gaacgccagc atcacagcgt actcggtcac agtcgcggcc
    19141 tccgattccg cgaagaatcg gcggcagcct gccgcatatt gtcttgctcc accgagcctc
    19201 atggttcgtc ctttcccatg cggcgaaata gctcgtacct aagtatttac gattcatttc
    19261 agccaggggc aaggaaggga aagactgtcg acccggtccg gaaaaacgtg gcgcggcaag
    19321 gttggccggc ggttggccga ccgccgggtt atcgggcaaa tggattgcag gacgcgcgtg
    19381 ggcgaatgcc ctgcatcatc gggcgaatcg cacaggaaag cccgatatcc agcccggcag
    19441 ggcgcgtggt cgtgacagac acgggggcaa tcgccacggt cttttgaggg ggctttttcg
    19501 gaagcccacg cgcaaggagc cggatcatcg agcagccata ttgtgaaggg gtgctgcctc
    19561 gcggttgcgg ccaagaagaa ccatgaaaaa ggtgaaagcc tcggtcgaat tcacgatttg
    19621 ctgtggccgg gtgcgtgaca gtctgggcgg gcatgccgac cggtccaagt tgggcgatca
    19681 accccgttca gcgggtttgc ctgccaaaac gggcttgatg gggtaacacc gtgaaggagg
    19741 aatcggccga ccgtgaacca gtcgtaaaac gaccgaccaa tttgcccttc gggaaagacc
    19801 gctgaagcgg tctgctttcg gcttgcgttc gcgacaaccg ggtcagccgc gaggagtgtc
    19861 gccgcttctg tcaggggccc cctgtggctt gtttcttccg gcgcccgttg tcgccatttc
    19921 gcaaccgcga tacactgact gacaagcatc ccgacgagca tggggtcggc aatcatgcgt
    19981 tcatgccctg gaagcctgcg tcgcgccgga gcactcaagg tcctgctgat tattttcttt
    20041 gtgatggtgc tggtttgcgc gggcttggtg acgctggcaa tcatgaatgg ccgcagcttg
    20101 ctggcatgga tgatatccaa gccgctgaag gcccttgtgc agcaatgttc tcttccggac
    20161 gatcagaaga ctcgcctcac ggcgagcctg gacgggctca tcgccgactt ccagaatgag
    20221 cgggtcagca tcgcccagtt gagcgggatc tttcaggagc ttgccgaaga gccgttcatc
    20281 gatctcgtac tgatcgagat tgtgcgaaat gaggcggtca aatcgggtca cttcagcgac
    20341 caggatctgg gggatctctc gctgagcctg gaccgtctac aaagaggaat catcgaaggc
    20401 atactcgaca gtgacgccgt taaggcgacg atggattgcg tctcgacggc acagccggac
    20461 ggcgcccggc agctcaagca gcccctgatg ccggcggaca taaagagact cgttgctgag
    20521 gccaccaagc aggcggaccg agcgggcatt ccgcgcgagc gatacttggc ggacatcgcg
    20581 ggggaggtga agcgggtgat ggatcggcag cttggcaaag ggacgagcac cgctccagcg
    20641 gccgtgccgg ctccaccgcc acctcagcct gtaggagtgc cggagcctcc tccgccgccg
    20701 gcttcgcctg cgttctcgac cgcgcccaac gcgatgacgg caatcccggt ggccgttccg
    20761 gtgcccgtgg cgccgccgcc cgcagcggtc gtcagcgagc cgccggcggc tgtctcacct
    20821 gcatctgccc cggtttcacc agccacacaa ccggtcgcaa cttcggccca gccatcgcca
    20881 gcccaacccg ctgagccgca gccaccggct tccgcagagc cgccgaagct cgttgaaccg
    20941 caaacggtgc cttcgacacc gccagccagc caggcggaac agacgacctc aatgcctgct
    21001 ggcgtagtgg aatcccggcc gtccgcagcc accgctgaac cggctgagtc cccggtgacg
    21061 gggcctgcac cggcaccgca gccaccctca accgcctcgg cgtcgataca accgtcttca
    21121 caaccggcgg ccggcccgcc gccacccgcc gcgtctgcgg cgcagcccct gccagcgcca
    21181 acgacgggtc cggcaatccc gccggcagaa agcgctcccg caatggccac acagcctttg
    21241 tccgctgccg cggcgctgcc aactgtcccg gcccccgttc cacacacact ccccggtcca
    21301 taggcttccc gaggattcat aatcgccctg ggtggctttc cgtcgatgaa ccgccggttt
    21361 tgggggtatg atgatgccat gcctgaacta cccgaggtcg aaaccgtttg tcgaggcgtt
    21421 ggccagcgga tcacaggcta cacgatcgag caggtccacc tgaatcgtgc cgacgtagtg
    21481 cacggggcgg cgatcccgct gtgtgcggca ctacatggcc ggcggatcaa aagcatcgag
    21541 cgggtcggca agcagatcat catcgtaacc gacggagcgg tcgccttgat ggttcacctg
    21601 gggatgaccg ggaagctcat agcggttcat ccggaaatgc cgatcgaacc acacacgcat
    21661 ctgcgaatcg gctttcgcgg ccgtaaggtc gaactccgtt tcgtcgaccc ccgccgattc
    21721 ggaggaatct ggttgctggg ggcggattca agccgagttg gatggatcgg gcggcaacgg
    21781 ccatcggtcg gcccggacgc tctcacagtg tcttgcgagg aatttgcccg tcgattggcc
    21841 cgccgccgac agatcaaagc cctgctgctt gatcagcagg tcctcggggg cgtgggcaac
    21901 atctattgcg acgagtcgct gcatcgggcc ggacttcacc cagccgtgcg agcagatcgt
    21961 ttgagtatcg aacaaatccg acgtctatac cgaacgatac ggagcgttct ggcacaagcg
    22021 atcaaggcag gcgggtcgag catcagcgat tatcgcaatg ccgacaatgc tccgggtcgg
    22081 tttcaagtgc gtcaccgggt gtacgaccgc gagggccagc cttgccggcg ctgcaagacg
    22141 gcgatcaggc gtctggttgt cgccggtcgt gggacgttca tctgtccaaa gtgccaaccg
    22201 gggccacggg ttggatctcc gaggccctta agaaggcaga cctgatcctg ttggcgtcct
    22261 ttgacaggca caactggtca accatcactt tcgcggttat aaatgtcccc gtcgggcttc
    22321 gttgtcggaa tgccctatgg aatcaacacc catgaaaaca gccaagctct tcacgatcgc
    22381 actcgtcagc atgctcctgg cagtcgccgg ttgccagatg cccccacaac cggtcggccc
    22441 gaccctcggc acggtgtccg ccgagacgcc ggaacatgtt gatcgtctgt gggaatcggt
    22501 ggaaacggca ctgttgacgt tcaacttcga cattgaccga cgggatcgcg tgcagggaat
    22561 catgaccaca tacccggaga cgtcggccag cggctttgaa ctctggcgcg ttcagccgac
    22621 accggcctac tactggtggg aaagcaacat tcacaccatc cagcgtcaag cggatgttta
    22681 catccgtgag gcgccggctg ggggtaattg cgaactcgaa gtgaaggtgg accgttaccg
    22741 tctcagcctg gaggagcggc aggtcgacaa cgccgccgcg gcgatgcggc tttacagccg
    22801 ggaggcgccc acgctatccg ggcagatgct tggatcatcg aagtctaccc gccggatcca
    22861 tcttggccgc gatggttttc tggagcaggc cattcttgaa gcgatcttcc gggaatacaa
    22921 gaaaccgacg acaacggggc cagcgggtga ttcaggttcg acggtggtca aggggggata
    22981 gcggaatcct gcgccgttgc cccgtgtggt aagccgttcc tcttgttgat gctgtacggc
    23041 gattcgtgat tttcttcggg ggtttcacaa tccgcgggtt ttcgggtata ctagcgcgtg
    23101 aagcctaaca gcgcagccag cgagggccgg cttgctcacg ggcatcggga tcgaggatgt
    23161 gatggtcaac aggcagcaag ctgaaccgaa gatatggaat tggcgggttc gtgccaggtc
    23221 gatcgacagc cagatcgtga cgctggggaa gtacgacacc gaagacgaag caaaggcgga
    23281 tcgcgataag attgtgaagg aagggttcta tcgcaacgtc agggtggagc aaatcaagcc
    23341 caaaggggga acccagcaac cgacaacagc ggagaaccag cctgccaaat gaggatgagg
    23401 ctccgcccgc tcctcgatca acaattatcg aatccacgct tgcaggcagc cgacaatagc
    23461 gatccgaggc ccattgaggc cgtcgtcggc agcgtgtcct gctctgcctt tcaggctatc
    23521 cgacggcaca agcctgccgc aaaaccagcc cgaccacttg tggggccggc ctgaggggat
    23581 tgcctcgccc gggacgggct acgggcaacc tctgggctgt ctctcggcgg ccgacagtca
    23641 gccccctgag cccggggccg ccttgccaca actccccggt tatggggtat actatagagc
    23701 gactcgggga gcgaggcaga ggctcaggaa ggtccgccac ttcatatcgt gggggcggac
    23761 caggtccggc gcagtcacgg cgtccgttcg atgccgggcc gcagttgtcg aaggcaaggc
    23821 aagattggaa ggcgattccg cctgttgcac agggggagtc catgtccgag cacgtagtcg
    23881 ttcgctgtca atcctgctta cagaagtacc gcgtgcctcc tacgtcggtc gggcggaagg
    23941 cccgttgtgt gaagtgtcac gccgagttca ttatcggtgg tgacaagccg acggacgaag
    24001 acacgattgt ggcgtggatc accgaggatg acccctcctc tcggtcggtg atgggttcga
    24061 cgggcatctt tcaaggcccg gaaacgcacc aggaagctcc gaaggcaagc gcatcgaagc
    24121 cggttcccat gtcggtcgct ccggagcccc tccaacaggt cgtgcacctt gaaggaatca
    24181 gggacaacgg cgcccatttc gagtttcccg ccgcggctct gacccgtgag gatctgagag
    24241 actccttccc acgcaagtgc gttggctgtt cggggcgcaa cgacttgcgg gttcatctgc
    24301 tttattggcc ggagcgtatg ctcggcaagg aacgcacata ctggaaagac cgcgttgaga
    24361 cagtggtggg tgatctcgaa cgcttcgcca actccgcggg gtcgggactg ctcaggcaac
    24421 tgcccagaac ccgacatctg gagcacccgt ttgacctgcc ttttcctgtc tttgcctgtc
    24481 gatattgccg gccttccgag gaagtggaga cccatgtcag cggcagacct ccgcacgacg
    24541 tttgccgcct gcggatcgcc tcgctgtcgg tggccgtcag tttcttccgc aataacggcg
    24601 ggcgcggctc gcacgcgtac cagcgtctta ttgaggaacg ggatcgtcgg caggacccct
    24661 ggcgggccct ggatccgcag atccgacacg gcatcttgca gtggttccag ccccagccgg
    24721 gagagacttt cgtgcgcttc atccacgacc gggatttctc gccggcgcag gccgcggtcg
    24781 ccgggtcggt gctgaccgat cgccgcctgg tcttcaagct gcaatctgaa tgcgaaggct
    24841 atccgctcaa cggcgagggc cgaatcgaga tcatgcgcaa gggtgacctg gccattgcgc
    24901 acatctacct gcatgggcag cgtccggcgg tgctcaagct cgggatcgtt caggcggacg
    24961 aactgctcga cagcctgcga cagttgcggt gccgctggac ggtcgtgaca tgagtcgctg
    25021 cgtcgctttc ccgaaggccg acggttcggg caggcattcc accaccggtc aggaagcgta
    25081 aaccccatac tcgcatagcg tttcccacat ggccatgtag ttttcgggtg ggacgtcgcc
    25141 ttgaacgtta tgaacagggt tgaagacaaa cccaccgccg ggcgccagat cgtcgatccg
    25201 ccgccggact tcgtcacgga cctgccgggg tgtgccgtaa gggaggactc gctgcgtgtc
    25261 gacgccaccg ccccagaagg tgatgtcccg gccatacagg cgcttcagct ccctggtatc
    25321 catgccttcg gcgctgacct gcacggggtt gaggatgtcg atgccttcgt cgatcagttg
    25381 cgggatgagg tctttgaccg ctccgcagga atggaagaag atgtaaacgc gggatttggc
    25441 gctggagcgg atatgctcga agagccgtcg atggcgcgga gcgatgaact cccggtacat
    25501 cgccatcgac atgatcggtc cccgctgggt ggcaatgtcg tctgcctctg agacaatcag
    25561 cacgttctcg ccgacggtct ccagggcctt cttccagtaa cgcattttca agtcggtgac
    25621 gatatccagc agggcgtggg cgaagccggg gtttgcggcc atgtcaacga ggaagttttc
    25681 aaatccgcgc agccataagg aaacctcgaa cagaccggcc gcgtgacgtc cgagaacgta
    25741 cgcttttttc tcttcatgca cgaagcggtc ggcccgctgc ttcatagtgg cgaaccgctc
    25801 cgggtcctcc gggtccggcc aggggtactt ctccagatcg gccacggatt cggcgtctgc
    25861 cagcggatgc ttgtacatgt cgaagtaaag gccgtcctgg accggcatgc gccaggtgat
    25921 gccccactgg tcctggtagc agtggtagtc gccctcgcgg cgaacggggg tggccaatgg
    25981 ggtttgcgcc tggcccgggt ccacgcaacg gacgtccacc atgaggcggt cgagcacatc
    26041 ctgatcgatg cgggcaagct gctgcttggt atcgtaggtt tgcacctcga cctccggcag
    26101 gccaaggtat tttcgcaggt tgcggtaggc caccgtgttc atacccgtca ggacggtgcc
    26161 gccgaggtca aacggcaccc gatcgggctc gcgatgctcc agggcggcct tgatccgttc
    26221 gtaagaggtg tatgtcatgc gcgactccgt tgtctacacg ccggccgtct ccgtttgctg
    26281 ccatcatagg agcggtacag gtgagccgca aacaatcttg ttacggtcaa agctgttctt
    26341 gccgaggcaa gcatcccaag ggatgatgtt ttcatggccg ccgcacaact gcacgtcgga
    26401 accagcggtt ggtgttacgc ccactgggcc caggggcgat tttaccccaa ggggctccgc
    26461 cagggcgaat ggctgcgatt ctatgccgag caattcagca ccgtcgagtt gaacgcgtct
    26521 ttctaccgtc tgccgaaaac cgagatggcc gcacgatggc gcgagatcac cggtgcccga
    26581 ttcctgttcg cggtcaagct gtggcggcgg atcactcatg acaagaagct ggaggattgc
    26641 accgatgaat tgcggggttt tcttgcagcg gttgagccgc tgggaacgaa gcgcggcccg
    26701 ctgttggtgc agttgccgcc ggggatgcga tgcgacgcag gccggctcga tcgctttctg
    26761 gaggagttgg gacgccaatc gacggggcat cactggcggg tgaccgtgga attccgcaac
    26821 gacacgtggc tggtcgacga ggtttacggc gtgctggatc ggcacaaggc gtcggtgtgc
    26881 cttgcggaca tggggcgttg cccgatcacc cggccgaacg atgttgactt cgtgtacata
    26941 cggcgacacg gtccggccgg gtcttaccgc ggttgctact cgctggatga actcaggcat
    27001 gatgcccggc agatccggcg gtggctgaat gcaggcaggg acgtctacgt ctatttcaat
    27061 aacgacgccg aggccaacgc ggtcaccaac gcgcgtgaac tcatcgatct gctggacggc
    27121 cgatcctgaa caggcaaaac cggccggcct acaggccgct ggggactgcg gcaactcggc
    27181 gccgtggcgt cagctccgct cggaatcctg aggtgaatgg gcctcaacgt cgatgagatc
    27241 gtccggcccc ggcggacgtc ctgaatccgg gccgggcatg ttaacgatcg tgaagccggc
    27301 ggcgaaccgc ctcttgagat aacgcctggc caaggcgcgc atcggcggga tcagcaacag
    27361 caaggcgatc acatctgtca cgaacccagg tgcgaccagc aagactccgg caaccagaat
    27421 caacaaggcg tcgaccatcc gatcgcccgg cagccgcccc gcctccaggt ccgcccgcac
    27481 gccgtgcagc gtccgcagac cttcatgccg ggccagagca gcgccgagta cgcccgccgc
    27541 cagcaccatc gcgatcgtcg gtccgacgcc gatgatgccc ccgaccttga tcatcaccgc
    27601 cagctcgatc agcggcatga gcgtgaacag gagcaggagc tggaaaagca tcgtatcagt
    27661 acttcccctt gtattcccgg accggatact tcatcgcgtt tttggccatt ttggcgcgca
    27721 gcgaagtggc gatatcgatg tccgccgtgt tggcaaagga caggacaaag gccagcacgt
    27781 cagccagctc ctcagcgacg tcggctttgt tcccggcatc tttgacccac tcgcgcgagg
    27841 gatcattctt gatccagcgg aaatgctcca tcagttcggc ggcctcggtg gcaatagcca
    27901 tggacagatt cttgggatca tgaaactgct cccagtcgcg atcccggatg aacgcggcga
    27961 tctcgcggcg aagctcggcg acggtggtat cttcatcggt cggcacggcg atcctccccg
    28021 gcggcacaac gggtgccgcg tgcgaaacag ggtaacatga agcaggagaa acacataaca
    28081 atcgtccgtg ccgggtgcgg cgacggagca attcgcccgc ggcccggcag gcgggctacg
    28141 gagccgcctg cgacgccggc aaagaggccg ctttcatcgg tggtcggttc gagatgacca
    28201 tccagtcctt gtcggggttg cccggatcac attttgcgcg ggcaatatcg tagaaagtta
    28261 cactcttgac ttcgtttctc gccttcttgt atttctcgta gctagccagc agatagacgg
    28321 tttcatcgcc agcgagcagg ttttcggctt tttttaagac gtgtgcctcg agcaattccc
    28381 tgacgcccgg cctggttcgg ttgacaccta tgaagccgac aacctcctcg ggcgtgacga
    28441 ggtatctcgg ccggcggccg tagtagaaac tgagtctcgc atcgggccgc gagtaagtcc
    28501 aatagatgct cgcaccggga gggacgccgg cctgatcgag agcctcatca agcatacgca
    28561 ctcgctcgct gttctcggcc gccagggtcg ggccgcagat ataccacgcc gaatgaaaag
    28621 cggcgacggt agccaccgaa gtcagagcca aggcccacca gccgtggccc cagacataca
    28681 acaggccagc ccaaagcagc acgaccagtg ctcccgccgc aatcaccgtc agggccccgg
    28741 cgaccttcgg gatgtacgca ttcatccaga aattcccgaa cacgagcatg aggatcgccg
    28801 acacggccac gatggcccaa gcgatccagg cgaaacgaag cggatcctga atgacaacgt
    28861 catgccagcg gccgaaccat atcttgagct tcacgggtat ggtcgtcggt acgaaggcat
    28921 agaagcgttc ggcgaccagc cccatcatca gcagcagagg cggcacggcc ggaagaatgt
    28981 agtacggctt cttgaaggac aacagggaca tcaccgccag cccgatcagc cagcagaagc
    29041 ctgcaaaaaa cagcgctcgt cgctgccagg catagcgctt cagccagggc gcggcgacgg
    29101 cctctgccaa caggaagagc cacggaaagg tcaggccgat gattatgggc acgtagtaga
    29161 atgctccacg agggcgcgtg tcatcgtaga agccctgggc cctttgccag tattgccagt
    29221 tccatatgtc ccaggcatgc ggaaactgtc tgcccacagc gatcatccac gggacaaaga
    29281 tggccgcgaa gacgatcagg ccgggcacca gatagagacg ggtaaaggcc ctggccgtct
    29341 gccggggaat ctgtcgcccg aagcaggtca gcacctgtct ccaaccacgc aaacccagga
    29401 acgccagcag ccgcaacggg cgctcggtat accaccagac ggccagtgga acggcggtca
    29461 gcgccatcgg cgccggcccc ttggccagca tccccgcgcc caaggcgaca tagaacaaca
    29521 tcatgtgaac gaatcggacc cacgcccggc gggtggtgac tccgtgccag aaatgcacga
    29581 gggcccacgt acagcagaga gtcaatagca tttccgccgt ggcgttgact gaatacatca
    29641 ggaaagcaac gctggaaccg gaaaccagcg cagtgaccgg gcccacgcgc ctgccaaaca
    29701 tcgatgaagc cagatgccag agcagcagga cggtcagcag ggccgaaacg gcggttggca
    29761 acctcgctga cacggtggtt accggaagat ccagttgcgg atcgttgggc agcaggtacg
    29821 aggctcccgc gacaagccag taaggaagcg gcggtttgcg catccagggc gtgtccagga
    29881 aagtgggcac gatccagtcg ccgctcaggc gcatgtttcg gccgcactcg gcaacgattg
    29941 cttcgtgatc gccaagtccg ggaccgcccc agattgcccc gagcgtggcc acgacaacca
    30001 aggccaggac ggcaagtctg atgagtatgc tgaaggtaat cgccgccaag cggttcgggg
    30061 gatcccgctg gacggagacc gacggagtct gtagggtgta ttggctcacg agggcacctg
    30121 ctgtaacgcg tccgctgacg cgcagtgcca ctatatgtcg atgaatggag cttcgcaatt
    30181 accgcgagga acgtcggcga agccggcgcg aggagcaggc tttggacaac tcccctcttg
    30241 tttcgatcgt cgtgcccacc taccgtgagg aagccaacct ccgccccctg gtcacccgca
    30301 ttgcagcggc gatggaatcg gccgaccacg aatgcgaaat cctggtggtc gacgacgaca
    30361 gtcgcgacgg tactgatatc gcagtgcaag agctggcgga gcggtatccc gtcagactga
    30421 tcacgcgaac cggtccgcgg gatctcagtc tggccgtcct ggaagggttg cgtcaagctc
    30481 gcggtcagat cctgctggta atggacgccg acctgagcca tccgccggag caaatccccg
    30541 agctgttgaa agcactgaat cagccgccga cggatttcgt gatcggcagt cgctacatgc
    30601 ctggcgggca cacggaggaa ggctggggtg tattccggcg tctgaacagc ttggcggcga
    30661 cgttgctttg ccggccgctg gtcggacggg tgaccgatcc gatggccggc ttcttcgcgc
    30721 ttcggcgcga cacccttgtg aatgccgacc cgctcgaccc catcggctac aagatcggtt
    30781 tggaactgat ctgccgatgc cgatgccgac acgtgcggga agtacccatc tgtttcgcaa
    30841 accgcacttg cggtcagagc aagctgaacc tggaccagca aagccgttac ctggttcatc
    30901 tgggccggct gtatcgcagc tactcacggg gctggggact ggtgattcgc ccgatcctgg
    30961 cggtcatgcg agcgggaatt gcggtcgtcg aatacctcag gtccagccga ccggacgtcg
    31021 tcgcctccat atcggtcgcc gtgcacaaga atcgagccgc ttgagggaag aatcgccggc
    31081 ggcacgggac aagccgtggc atgaggccgg gcgccgggcc gcggcgattg cgtcgtacaa
    31141 cctcgcgaag cttgccatcg accgttgcga cagtcgccga gaggcaggcg gtcacttctc
    31201 atctgcaaat cgagcggggt tttctgctga tcagaagaaa gagaaccgcg tggcggctcc
    31261 gcccctgccc gatgccagga atgatccgaa aaacccgcac actcttggag caacgacaag
    31321 gagccaggct gcgggatcgc tgagaaaacg ggcggacgtt gttgtccggc ctggttcggc
    31381 gcggtatggt gccgcggaag cgcgccggca tgaattactc gaccgggacg ctgctttttc
    31441 cggacatacg gccgttatga ggttccgggg ctttggaatg ccgaccgggc atggcggggc
    31501 gctgttcatg agcggcgtcc tgaccctcca atcgtacggc cgctggagtg cgtgacgtgc
    31561 agaagctctc gatcaccgag actcccaatg aactgacgac ggacatcgac gtggcctcgc
    31621 cggtcggcat tgtgcgtatg cttcgggcgg ccgatgccca gatctatagc ggttatcgcg
    31681 actaccccgc agtctgcgac gccgagatcg tggccaagat cgtccggacg atcgaatggg
    31741 ccgcgccggt gctggggcgc gaggattccg ttgtggtcat ctccggcgcc ggcacgagcg
    31801 ggcggttggc gatgttcgcc gcacggacgt tcaaccggtt tctcgccggc tgcaaacgcg
    31861 cgcctaacgt tcgttatctc attgcggggg gcaatctcgc gctgatcaag gcccaggaag
    31921 gcgcggaaga tgatccgcac aaagccgttg acgatgttga gccgcttgtc agaggcaaac
    31981 ggcgcgtctt atacatcggt gtgacctgcg gcctgtcggc gccgtatgtg gcgtcgcaga
    32041 tgctgcatct gtcgcggcgg cgcggcacga agtgtgtgct gatcgggttc agtccggcgg
    32101 atcgggcgcg cgatgtcgaa gtcgaggact gggacaagac gttcgcgagt gctttgcagt
    32161 cggtcttgcg gcgcaggaac ttcctgctgc tgaatccggt tgtcggcccc gagccgatta
    32221 cgggctcaac gcgtatgaaa agcggcagcg ccaccaaatt actgttggaa gttattttcg
    32281 ccctggcggt cgagcagggc gggcagggcg tgctgccgga gcgcgtggcg gcgtgcctga
    32341 aagcctatga gcagacccgc gtggccgtgt acgagcagac tgccgcgatc ggccgactcg
    32401 tggagctggg tggtcgggcg ctgcgacagg gcgggcatat ctactacctc ggcgccgata
    32461 cgccgggcat tctgggcacg gtggatgctt ccgagtgccc gccgacgttc ggagcggatt
    32521 tcgataccgt caggggtttt gttgtcgggg gttggcggac gctgttggga ccgggccacg
    32581 acctgagtga cgagggtccc tggtatcgca tctcgctcga cgagttcaaa caggtcaaac
    32641 tccccgccct gagcggtaag gatcttgtcg tcgggctcgg catggacctc catcggcagg
    32701 tgggcgaggc gttgcatcgc agccgcaacg ccggtgctcg gaccgcagtg gtgcaggtgg
    32761 gccggcgggc gcggggacgg gttgccgttg atgcggcggt ctgtgtaccg aacgtgccga
    32821 agacgattat cccgggcgcg aaggtgttcg aagagttcgg cacgaaactg atattgaacg
    32881 cgttgaccac cgggggccac gtgctcagcg gcaaggtcta ccaaaaccgc atggtcgacc
    32941 tgcgtatcag caacaacaag ctgttttacc gaacgatcgg cattattcag catatcagcg
    33001 gggtctcggc cgaggcggcg aagcgacatt tgctgcggag tatctacggc accgaccggg
    33061 tcacgcccag gatgatgaac gccaaaccgt cgcagcatgt ccaggcatcc acgaacgtga
    33121 cgaaaatcgt ccccaaggcg ctgatcatgg ccatgggccg gacaacgccg gctgaggcag
    33181 acaggcttct gaaaaaagaa cccatcgttc gcgctgcgat tgaacggtta aagagtgcat
    33241 cgacctgaac cgcaggtcgc cagggagaca acacatcccc atagaccctt tgcactgagc
    33301 ccttgccctg tcatacacag gattgcttcg gcgccaacaa accaacacct gagggagacc
    33361 gccttttcgc gggccgcaat ccaagcgcgc cgtcatccgg aaacatgcgc gcaatctgca
    33421 cacacgtctg gccgtaaggc tctcatttgc gatcgcggtc gggatctcga acctgtggtt
    33481 cgggcggcag cgaaccaagt ccaaggccaa gaaaccgtcg tggccggatt gtccctgatg
    33541 atgataaggt caattcgtac aaggacttgc gcgatttaac gactagcggc gagtggattc
    33601 gaaccactga ccttgggatt atgaagccca cgctctaacc acctgagcta cgccgccaac
    33661 atattggctt gccggcactt acgatttctt atcggccggt gccttgctcg tgcgacaacc
    33721 ggaatcccga cctgccagct ctgtagcacc cttctctgag cgggcaaagg ccggcgacta
    33781 ccacggctcg tacgcactcg gcgacacaaa ccgcgaaatc ggacccggct tgccagccaa
    33841 tccgctcgcg ggcggccctt cgctggcaag caagcccgcg gccgggtcag cctcgccaat
    33901 ccgcgagcaa agccataacc aagcagaatt ataaccgccg acccttcaat ttgcacgtca
    33961 gcccacccaa ccccggccgg tcttggccct gccagtaatg accggggcgg tgcgccggca
    34021 ggatggacga agcgagagct catcttcctg ataccgaatg cggcagcagg tatttgtgac
    34081 cgcagccacc gagaagcctc tggtcgtcag cccgcccgaa cggtataaag agccctcttc
    34141 cgaaaaaaga cactgcccaa atgactacga gggatgccgc atgaggatta ctgctattga
    34201 aacgcacgtc tgcaatgctc gaatgcggaa ctgggtattc gtcaaggtgg tgaccgacca
    34261 gccagggctg tggggctggg gcgaggccac gctggaatgg cacacgcggg cggtggtcgg
    34321 cgcgatccag gacatcgagc cgctgctgat cggcgaagac cccacgcgtg tcgagcacct
    34381 gtggcagatg atgtaccgtc agcacttctg gcacggaaat ggaatcatcc gggcgacggc
    34441 catgagcggg attgacctgg cgctgtggga tatcgtcggg aagatccaca atgtgccttg
    34501 ccacaagctc tggggtggcc cggttcgcga ctacatccgc acctattgcc acctgggcgg
    34561 cgggaagatg gaagacttct atgaaaccga tccgaaagat gcgacgcggt tcgccgaact
    34621 ggcccgcaag gcggtcgagg acggtttcac ggcgttcaag accatggccg ttccgtggac
    34681 catgccgatc gagggccttc gaccgatcaa gtatgccgag gcctgcgtcc gggcgatgcg
    34741 cgaggcggtc ggtgatgaca tcgacatcat ggttgattgc cacgcacggc cgtcgccgcg
    34801 gatgggcgtg ctttttgcca aggccctgga gccctacggc ctctactggt tcgaagagcc
    34861 ctgctggccc gagactgttg acggactggc cgaggttcag cgggccgtcg tcacgcccat
    34921 cgccaccggt gagcggctga tcagccagca cggcttccgc gaactcctcg agaagaaggc
    34981 gtgcagcatc atccagccgg acatcaccca cgtcggcggc ctcagcgagg cccggcgagt
    35041 ggccgccatg gccgaggtgt accgcgtggc catggccccc cacaaccccc agggcccggt
    35101 cagcactgcc gcgtcgctcg agttcggctt tgccaccccc agctacatca tctgcgaggc
    35161 cgtccacctt gacgtcccgt ggcggcagga cgtggtcagg gagggcttca ccgtcgaaaa
    35221 gaaaggccgc atcgtccgcc cgggcagcaa gcccggtctg ggcatcgaga tcgacgaggc
    35281 cgaagtcaag aagcacccgt tccagcagga actgccccag cgagtcttct actccgacgg
    35341 cagcgtcggc gactggtgag ggtcactacg ggacgatgat ctccggctcg cacgtagcct
    35401 tggacggaag caactccgtc ttcgtgcccg acgggaacca ccatttcgcc cggcgggcct
    35461 cgctcccctt gccgttgttg gcgtcgctgc tgccctcgcg ctggcgctcg acgtggccgt
    35521 cgaaggcggc gatgttgatc gtcccccgat gtcgccacat ttcctttcgg aggaaatcgc
    35581 cacgataggt cactggcccc tcgtcgtgcg accgccacgc accctgcgac gaaaagcccc
    35641 agttggcgtt ggcgatctcg cctttggttt tggacacgat gtgggcatca gccagaaagg
    35701 ccttgcccgc cggctgcttg atcagatccg gtttgggcac gtaccgcgcg gggatgcagt
    35761 cttcatgcca ccagagggtg ccggccgtct cgccggattt cgccttgtcg ccgacgtagg
    35821 tgaagtatcg cgacatggcg tagcttgggg caagtgtgtc cgcagggatc agatcggtca
    35881 gttccggata gttgaccggg ctggttctac ggcggttcgt cgggcactgc ggaaccgcgc
    35941 tgacggcagc cgtgtagcgt tgtttgaagt cgcgcggagc gcgggtgccg aactccttca
    36001 gaaggggact ggcccaatcg aagatttgca gggcgggata gtgatcacgg ctggccgtgg
    36061 gcgtgtaggc accggcagcg aaaccgggcg gcgctccgtt accgctggtg ttaggcgaac
    36121 cgacgagcca gcccctttgc tccaggccat aggctgtggc cgcaaggctg atctgcttga
    36181 ggctcgaccc gcacatcgcc gctcgtgcca gttccctcgc ccccgacagg ctcggcagca
    36241 gaatcgccat cagcagagcg accattgcca ccacaaccag cacttcgatc aacgagaaac
    36301 cggccgggcg cttactgttc tgtaaagagg taaaaggaga gtacagcaac atatctgcct
    36361 ccgaaacggc agtatggtat gcaagcacta ttgaatggtc aacaattcta tcaagataag
    36421 tgctgaactg aaaagaaaca agacggccac gcgccagagg cgcacagatt gccgaggcat
    36481 gggaccgctg aggctgtact catgggaagt tgttcctgcg gcctggaggc atcaacagac
    36541 agagaccgcc tgatgctcaa ataagaggca ggctgtcgct ggtatcgtac tatccgcagc
    36601 ggccccggat cagcgagacg catcggcgcg ttggagagct atttccggca gtttctgcgt
    36661 cgattcttcg tcagacgcgg gcgtactcca acctatgagc cgctgtgcat cgttcaggat
    36721 cagatacagg caagggataa tcaggagagt tatcgccgtg gcgaagagga tgccgtagcc
    36781 cagggacagg gccatcggaa tcatgaatcg ggcctggcgt gacgtttcga agatcatcgg
    36841 agccaatccg ccgaacgtgg taacggttgt cagaataatc ggtcgaaagc gtcgaacgcc
    36901 ggcgctgagg atggcctcat gggcgctcag accctttgtc cgctgccggt tggcgtactc
    36961 caccaggacc agcgcatcgt tgacgaccac gcccgaaagt gccacaagac ccatcatgct
    37021 gatgacgctc aggctatagc ccatgatcat atgccctaaa agagcgccga caatcccgaa
    37081 ggggatgctg atcatgacaa ttgcgggctg gaggtagctc cggaaaggta tgccgagcag
    37141 cacgtacacc agcatcagtg cgatgacaaa gccgctttgc aggcttgcca ggctttctcg
    37201 catgtcttgt tgtctgccct caaagctata ggtgaggccc ggatacttgc tcatcaagtc
    37261 ggggagagtg gtctcggtca gcatctcgag aaccctgtcg gtttcgctct gcggaaccac
    37321 attggcggtg acgctgacgg tgcggcgtcc atctcgccgg ttgatagcgg tgtatgcccg
    37381 accggcttgt acggtggcca cttggtgcaa aggcacatcc gctccagccg gcgtccgcac
    37441 aatgaaggac tccacgtcga atccgctgat gcgctgggct ttcggcagcc gcaccatgac
    37501 tttgacctcg ttgcggccac gctgctggcg gatggcctcg gcgccgtaga aggcccctct
    37561 cagttgccga gcgacggcgg cggccgtcag cccgagactt cgtccctcgg ggagcatccg
    37621 gaaattgagc tgttgtttgc cgggcgtata accgtcgtca atgtccctga cgttggcgaa
    37681 atgggccaaa gacgccgcca gttcctcact tgcccggtcc agtacctcga tgtcacgatg
    37741 ggccagttcc acggtcatcg atgccccgga accgggtccg cgtcggtccg attcaaaacg
    37801 aagcgattgg agtccttcga tgacaccggt cttctctctc cagagacggg cgaactcagc
    37861 ggtgctgatc ggcctgattt cgggtgccaa gaggtaagcg gtcacgaaga tctcgttttc
    37921 gcgaatgtag gcccgtactc cgcgcatgag ccgctcgccg ccgttttcgc cggctacgga
    37981 ctgggccgac cggaccagac ggtcgctgac ttcctgcaat ttcgagagcg gagaaccgta
    38041 aggcagaatg gccgtggcat cggcttcgtc agcctcaacc ttgggcatca tctcaaagcc
    38101 catccgcccg ctggcaacgt aaccgagagc cagaaccagg attcccgccc cgaggctcac
    38161 cgtcaggtaa cgaaaacgca ggactgcggc cagcagcggg ccgaaacctt tctcaatcat
    38221 gcgtgatatg agcttgctca cgcggtcctg ggcccggtga atgacttgcc ccaagctggt
    38281 ggcgttctcc ttcttggtgt gggccaggtg agagggcagg acgaacatgg actccagcag
    38341 agaaatagta aagactgacg aaacgaccag tgggatgacg cgccatatct tgcccatcgt
    38401 cccgggcatg aacgccaatg gggcaaacgc agcgatattg gtgaggatgc tgaaggtgac
    38461 cgggacgccg acctccttcg cgcctcggat ggcggcttcc aagcgtccca tgcccgactg
    38521 acgatagtcg aagacgtttt cccccaccac gatggcgtcg tccacgacga tgccaagagc
    38581 gatgatgaac gcgaacattg agatcatgtt gatggtgacg tccatggccg gcaggaggag
    38641 aaaggacccg agaaacgaaa tcggaattcc catggcgacc cagaaagcca acttgtactc
    38701 caggaagatg gtgagaaaca ccaggacgag cgccaagccc atggccccgt ttcgcagcag
    38761 cagatcgagc cgttggcgat agatctccga ttcatcgcgg cggacgtcga tgtacactcc
    38821 cggcggcagc gcgggccgga tctcctccag cttcttccac accgccttgg agactccgat
    38881 cggcgtttgt ttgcctacgc gatacacctc caggttcacc gccgtcttgc cgttgtatgt
    38941 aacgaaacgg tattcctcct cgaaggtgtc ccggacggtg gccaggtcgc gaaccagcaa
    39001 ctgagttccg tccgcggagg tgatgatcgg gatgttctca aattccctgg cccaatcgcg
    39061 tcgctctttc atccggagca ggatctcgcc cgaatcggtt cggaggctgc cgccaggaag
    39121 ctcgatggag gcgcttcgaa tccgctcggc gatggcctgc agggtcaggt tgtacgcccg
    39181 gaggttttcc tgcggcacct cgatgctgac ctcgaaggtg cgcgcgggtt ccaggtccac
    39241 ctgggtcacg ttcggatcct gcagcaaccg gtcgcgtacc tgttcggcca gctctcgcaa
    39301 gatccattca tccgcatcgc catagagaat gacatccatc acctcgcgcc ggtgcgtcat
    39361 caggacgacg tcgggctctt cggcatcttc aggaaatgtg gtgatgcggt cgacctcctg
    39421 ccggatttcc tggtaaacgc gctgggcgtc ggtgttctcc tccaactctg cggtaaccat
    39481 gcccgagcct tcgcgagcaa cggcggtgac ttccttgacg ccggtgattc cgcggatgcc
    39541 ctcctcaatt gccagaatga tcccctgctc gacctcctcg gggcttgcac cgggataggg
    39601 gacggtcacc atcaccatgt cgagatcgaa ttcgggaaag ac
//
LOCUS       DAOTPW010000013        38840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1143_length_38840_cov_5.105170, whole genome shotgun sequence.
ACCESSION   DAOTPW010000013 DAOTPW010000000
VERSION     DAOTPW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 38840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38840
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1143_length_38840_cov_5.105170"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="alr"
                     /locus_tag="P5316_02040"
     CDS             complement(<1..924)
                     /gene="alr"
                     /locus_tag="P5316_02040"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRR83766.1"
                     /translation="MHEPATAYVSAAAIQGNVEAVRRRIPPGTPICVAVKANAYGHGL
                     SQVLPALVRAGIERLAVANLDEAVELRALGWTGPILCLGPILASRSESERIENAAEAV
                     AAHIAVTITSPEEARLLATAAKRQHRMGHVEIQVDTGMGRCGVRCDKAPDIIAQTALV
                     PGILIDGVYMHFATADEDDLRFARSQLQEFKQLIARMREAKLAVGSFHAANSAAVFRL
                     PESHLDLVRPGLCIYGYWGGPASERPPDLRPILRLVVRLTEIRRLPAGASVGYGCTWR
                     AKRESVIGTVSLGYADGYRRVLGNRAIVTLET"
     gene            1047..2351
                     /locus_tag="P5316_02045"
     CDS             1047..2351
                     /locus_tag="P5316_02045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRR83767.1"
                     /translation="MATAFACLEMRKHHEPPAAFAELGIQTPFLSALQRMGFSRPTEI
                     QQRLIPLALAGRDVLGQARTGTGKTAAFGLPLLQLAKPAEGLQGLVLVPTRELAVQVA
                     AEMLRLAETSGLRVVPVYGGQRIRQQVHLLGRKPHFVVGTPGRVMDFLDRRILSFSGI
                     RFVVLDEVDRMLDIGFRDDIKRILSQVKTAHQTILVSATMTDEIKRLAEQYMTDPVEV
                     NVSQDKMTVDEVRQSFYTVDRWDKYRLLRTVLDSRKPKLAIVFCNTKHAVRKLAKRLN
                     GDGINAREIHGDLVQAKRDRVMSRFRKHAIHVLVATDLAARGIDVQGITHIINYDIPD
                     DPLGYVHRIGRTARMGCFGEALTFVTREEGKQLTEIEKLINREIPCEQMEGFEPSPPP
                     EQHSAFAIAAKPEASVDAPAANVSTAPKRLGAKFRPVRRRRL"
     gene            2503..2730
                     /locus_tag="P5316_02050"
     CDS             2503..2730
                     /locus_tag="P5316_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRR83768.1"
                     /translation="MPIYEYRCLKCRHEFGRLVRSDAEEQALVCPKCGKKELEKKLSV
                     FAAGKGAASEPSLPPSCAGCQNLGGPCPMRQ"
     gene            2757..3221
                     /locus_tag="P5316_02055"
     CDS             2757..3221
                     /locus_tag="P5316_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83769.1"
                     /translation="MKTSLAEDNPQLRMSVAAVLTWLMPGAGHLYIRERARGLIILVT
                     IAITFWVGVAVGGLSNTVSPGGRSLWFMGQICAGGHALAAWGLGTWVDPSPVPKRDPK
                     FVAFGRSEEISVVYTAICGMLNILVIFDVLVRAEKPLIQAGRTPPAAKGAAG"
     gene            3218..3448
                     /locus_tag="P5316_02060"
     CDS             3218..3448
                     /locus_tag="P5316_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83770.1"
                     /translation="MIELLATLMINPLPLVRWQQALLLLPLCLCISIIYKTIKCENLR
                     EIPMAVLKNWITIVIGMYVVGIALLLIYELLA"
     gene            complement(3526..5844)
                     /locus_tag="P5316_02065"
     CDS             complement(3526..5844)
                     /locus_tag="P5316_02065"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRR83771.1"
                     /translation="MNAMIRRFCSEGAMLIGVVVLLASAERSAHPENFWLYVILTPAS
                     GVAGLWSLLYRGRPLLSAHTALVLGVVAFVFLIIEWGWRFSVPTVLALAHFMILICIT
                     KWLQWRTHRDDAMLLILLLLLLVVGGIVGGSLLFPLVLAVYAIAGCQRFITHHLESEA
                     AWSDRRNVQASGFRRPDGTTSHAGLNAQRTGSLSVAIVTLGLGVMVFVAIPRVGAGMF
                     GQAGQPATAGALSGFARSIEFASVGAIEESDQPIMRLTATDEYGTPVGSRVPLYLRGE
                     VLDRYTSRSPTGNRNWGWRRSGVTELSADTYQLTSDEETTPSTTLFRNDIALPHGPML
                     IQKYRLEPSDDITLFACYPPLEISSPTLRTVRKWNGTQILQTLRPVRKQLDYTIKSPA
                     VPAMAAKALAAERGAADVPPPTPPPIANERREQLLRLIDEQTRGIGPLTEPGNRLSFA
                     RRLEAFLQSEHFAYSLEPPPMPETGDPVSSFLLVSRKGHCEYFASALALMCQLKGVPA
                     RVVIGYLANEYNSFGGFYLIRKKHAHAWVEVFIPGLDWVTLDPTPLSRRQSGRLTGLG
                     ARIRAYLDFLQFQWSDGVLSFNAETRRRMLDRFAQWLRRPAQDQRTLIGGVVAFIREL
                     FGWRLALSWHERLVYWVFTLLVVTLVLLLTYVAVSLARRLTRLARRLSQGRHARQASR
                     SREVEFYHRFCRFLADLGLTRRPDQTPAEFAADLAERYAILQEAPVLVQCYYDVAFGG
                     HALTSQHRGTIEDFLRRLRHLTVDQLTTPQTQ"
     gene            complement(5841..7103)
                     /locus_tag="P5316_02070"
     CDS             complement(5841..7103)
                     /locus_tag="P5316_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRR83772.1"
                     /translation="MTALADRCILLEPQHKRLDAPRAQTARRTLHFTFGGLLFAFVNV
                     VVLLAGLNSEANLLVLLFGIGVGALLVNAVFPILMLRRIEVDRIAPEGVVADRPFAVA
                     YRLRNRRKRLHARGLTVTEMRVNDRPARFPTVFVECLPPRQEKRVEALGQCPRRAHLV
                     LTGIRVSCGYPFGLFACVLEAPSAGSLTVYPAMGRMRHDLWKKTAGGQSQSARTARER
                     ENPDEFVGVREYREGDNYHWIHWRRSARTGELVVREMLSPRQTRMIIILDPWPDAAGN
                     TSRPQQDTDDHDGRAERLISAAATAACAALEQGHRVGLICRAAKPVVIAPTAGKVHRQ
                     RLLTELAGIGPGAQTSFDELVGRVHWSTGWNARCLLLSSHIRSVHQRVARIIGTRAET
                     ILVLSPDSEPFDLLFDLTHPGAPVRRRR"
     gene            complement(7144..8106)
                     /locus_tag="P5316_02075"
     CDS             complement(7144..8106)
                     /locus_tag="P5316_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRR83773.1"
                     /translation="MEANLQLAGDPLLAKVAELRNNIDRVFLGKPNTVTQVLVGLMSR
                     GHLLIEDAPGVGKTVLARALARSLDLSFSRIQLTPDLLPSDIIGVSIYDAKTGTFEFK
                     RGPIFAHIILADEINRTTPRTQSALLEAMNEAQVSVDGRTLKLEQPFLVIATQNPFEF
                     EGTYFLPENQLDRFALRIRIGYPSREVEARIIREDPSKTALEVLRPVMRREDLIAIQD
                     RVPQIRIDDRLVQYVLDLLEITREHEHLELGVSPRGTLAFIRAAQAAAMLAGRDYVIP
                     DDIKSLFIPVCAHRIATKSFVHEGHTISAEQILTEILRTVTVPE"
     gene            8309..9916
                     /locus_tag="P5316_02080"
     CDS             8309..9916
                     /locus_tag="P5316_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRR83774.1"
                     /translation="MMHGLKTWMPVLLAVGWIVVSSGALLAAEDTSSAGSKVNIRTSG
                     GMRIVAVRRPASSPAINTQALAAPSSVVTAAEPMTAGTLSTPFPRPPLAGFSPLVAIT
                     TSDKREFDDLMWDHTVHHTYTGNALNAPANANFIIGIFDSGSVVDLVAEPWDWIVGLT
                     GTRLTGNPFPIGGTGGTVDAIISKPVGYFANGLSAVDAASGLLDLTKVVGHGNTCLLV
                     APEIACGNGEGLTAVLGTGFVSFFNTIIRVDTPIRRGFGGDDVLSPSVQIQDQSIALP
                     LYPRKMSMTFSSGGLPVTTASYYGLLDPWTWEVDIILPTLLGMGSGMIPLGGYFFGTI
                     GAIEGEPGPTNPLQNMRVLVDTGAQSSIITPGMAANLNLDLYNPDFTVDICGIGGLTT
                     DVPGFWVDYVKMNAAGGALEFSQAPFVVVDMASPDGGQFDGVLGMNFFWNRNVIFQPV
                     PSGSSFLHISDPIPFAYGDLDRDLDVDQDDFEIFSACSSGPGIPFSAECTLVDADDDG
                     DIDQTDFGFFQACLSGENVPADVNCKP"
     gene            9934..10953
                     /locus_tag="P5316_02085"
     CDS             9934..10953
                     /locus_tag="P5316_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRR83775.1"
                     /translation="MFVQRQLLLVLTVLVGLALIAAVPSPLRPGAAFASAGDEEGVQQ
                     ETTMAGKTPKQKTRPAAAKTGGGDDTAAGRQSTKPAKADAGQIRVAICQILCIDGDAE
                     GNFRRIENALEVAAAAKAQLACFPESAILGWENPDAFKLAGPIPGPHSDRVAALARKY
                     KMMISIGIDELAEPGCLYDSAILVDEKGKILLKHRKLNVLPELMDPPYSVGKPEDIKA
                     VDTPLGRIGMLICADTFTEEYVKRAGSLKPDVLLVPYGWAAPKEKWPGHAKNLHKTVA
                     QAAQWAGCPVVGTDLVGMITHGPWTGQTYGAASVVADRQGNILVILRDRDVDVQVIDL
                     AIGRR"
     gene            11405..12115
                     /gene="bioD"
                     /locus_tag="P5316_02090"
     CDS             11405..12115
                     /gene="bioD"
                     /locus_tag="P5316_02090"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRR83776.1"
                     /translation="MPGWFITGTDTGVGKTVIAGALAMLLREHNRKVAVFKPVATGCR
                     RDVRLGLASEDAEFLAHCAESDATLETINPVRYGGDVAPMIAAEHTRKPVDWEAIDQS
                     WCRLRASADWVLVEGAGGLLVPVDPKNNMADLAKRFNLPLIIVARPGLGTINHTLLTI
                     EAARTRGLPISGVVINGYRPDSATLAEETNPEVIARLAKISMPLIAPFDARTDLRQGL
                     VGETLLHALRPFVERALG"
     gene            12605..13552
                     /locus_tag="P5316_02095"
     CDS             12605..13552
                     /locus_tag="P5316_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR83777.1"
                     /translation="MIEVRNLTKFYGSHKAVDNISFSVEEGEIVGFLGPNGAGKSTTL
                     RILTCFLPATSGSASVAGFDVFTQSLEVRRRVGYLPESNPLYQEMRVREFLNFRGKLR
                     GMERSAREAAIRRVADRVWLGDFIDRPIGQLSKGMKQRVGLADALLHDPKVLVLDEPT
                     IGLDPTQIRETRKLISDLARRHTIILSSHILPEVEATCQRTIIIAGGRIRASGSMSEI
                     RDRIKGASRVIAEIRGPKEDVARAIRQVNGVKNVEINPNEGWNHLRIETSEGRDVREE
                     IASVAARHNWGLRDLRLETGGLEEFFIQITAEAAGSTGR"
     gene            13607..14377
                     /locus_tag="P5316_02100"
     CDS             13607..14377
                     /locus_tag="P5316_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR83778.1"
                     /translation="MSEQNEAKVPSAAGATSSVAVVALMERELSALFYSPIAYIVGFV
                     FLVLSGMYFTTDVLQPGNEASMRLLFERIAGLLVFALPLLTMRSVADEFASGSIESLM
                     TAPVTDAGVILGKFFGALIFYIGLLATTVPHLILMTIYANPVGTEIVSGYIGLILLGA
                     MFISVGLFASATTRHQLLAAIIAVAVLSLFSFAAEYATAQVTNVWLSKFLKSLNILDQ
                     FSNCNKGMIDSASVVFFVSNTCLFLFLAIKVLESRRWR"
     gene            14368..17118
                     /locus_tag="P5316_02105"
     CDS             14368..17118
                     /locus_tag="P5316_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83779.1"
                     /translation="MAVNDKHHEMSATGRRLFFGTNVFIVILLLAAVVVGINFLFTDP
                     RLNKRWDLSAGSSSYRLSQRTKNVLKQIEGDKIAITTVYTSVEPEYERKTYFPRVEDL
                     CDEIAQFDKRVRVTHCYSPADKLALRNRIQGKFGGAAEKHNEVIAAARTLWEDVQQSL
                     PPLQSQIEGLQRDDNAWLGGFSSLNRINLNLKANLENIAETRRDVQDLTEGQDAMPKY
                     QEATNKIKQTNDQLKQTLEDAQKWCKDINSLATVLSNKDSDFAAKTRKQLEELDKLLA
                     NLVAGVGDPSDTTIPDDPKPAIQDFAKASNALGRWLTEEVGRVNSFVKANPAIEQHPR
                     WMLNVQLGPLVAKMPLSANLSETAQSVSENVQAVRQVLSAPNVPKDQLQSLLRQLRQI
                     ASNIGQKLDKEWKSSILAILDDGARIDQASRDFLARGSEGTMFKEILDKIADIAKKIE
                     SLPKLELDEIAERLQDENLVVIEVGDQVRVVKFDEVWPIADPTMRMRMDESRTARVFD
                     GDSAISGALLAMTRQKKYGTVIFVAYETEADPMARRMGQGGRNTGRIPLEQLSTLKDK
                     LKQANFAVKDWNLGAEGTDAERPQPEEGTEAIYVFLPPAETSPPNPMMGRPPEKGFGE
                     EQLEKIRPLLTAGSKAIFLATWVIPRMSWFGPEPVGPYAYGDILSKNYGVEVHQSYRL
                     LRVLPDTHDPTLFGIDIEQVGHMQLSSFSDQPIGAPLKARRMLMNDVCPVTKVKGQEP
                     PADVSVSTILEVPAVLNYIWAFSDKGIQMISKALSTGENRSQFRDDPDSIWKPPFSVA
                     LAIEKSFGENEPKSRVVVLGTGASLTDWHLTRRVPRFLGEGKRARFQTDPPPTENADL
                     FVNAVLWLCNQEEMIASGPSQIPLVPVLKAGTQTKVLTVTMVWAAIVFVAGIVVMLVR
                     SK"
     gene            17125..19794
                     /locus_tag="P5316_02110"
     CDS             17125..19794
                     /locus_tag="P5316_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRR83780.1"
                     /translation="MNTKTTLILAILAVAVAACLFFVVKPWETPVSGPVEEPKTTAKA
                     MFDPKPADVDRVEVLVRGEQSPRVFKKETDGWMMHAPTTAPATEWEITNNIIDKIGNL
                     KYLKEYPVGDKNRPDDNATRFNNPVATVKLMKEDKVLAELVVGNSLPTGRGNFVKKGG
                     SDVIYESQESLSDAFDKKISNYRNKRVVNVKLDDVKEITASGLSAYKLVKSGSDWLIE
                     QPERGRADKSAVETNVANPLISLYVADFVDDTPASYKVYGLDEPRLTLTLKSTRTVPP
                     KAKPGDPDTKPADTQPSTEEVENVLLVGGSTGENKYYARLKSAPWVFTLNAGTVDNFA
                     KKVSDLRDKTLAKVESAKVIRVVSKTPDGEMTLVKQQGKWSFADGTASDATAVSDLIK
                     ALADLKAVDFVDTSKLLMPLDWNNPRAAVTVTQEGEANPVTVLVGPESSSGKMVYVKN
                     AAEDAVAAVREDQVTQLLAGPIAYGDRQILEFPRERATRLEIARAGAETVVLKQEKNI
                     WSMISPVQASVDADAARNLTQDLSTLRAKGIVGKDKKAFGLDSPTVTLAVYVEPLTAD
                     PKVKVASSAPATTKPATTKPATTQAGKKPTLQDLLDYTLTLPKEKQNPLAIEMLKDMI
                     AKEKAEATQPASQPAATKPAKYAPGMSPEELLEFQKSLPDDQRNPKATEMLEKLVAER
                     KAAESRTAEEQPAASQPEPPPPPPTIHRLVLSQKGGKTYACREDRDLVYELENKIYDD
                     ATAEMHDRQVARLDTATVTDLTLVVGGSELSFRKSGDEWTYVADPVLPIDKTKIDEVL
                     NAVRDLKTHRYVAYQADDLAKYGLTGQVDRVSLVADGGRRIEILLSPTGPAGDTDKSR
                     YAVLSGSKAVFLLKDDQVNKFTKKLDDFEKKAS"
     gene            19855..20142
                     /locus_tag="P5316_02115"
     CDS             19855..20142
                     /locus_tag="P5316_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRR83781.1"
                     /translation="MADSQDRVCCTVLFAGAVQGVGFRYRTERVARDYEVTGYVRNLP
                     DGRVELVAEGLRPEVERFLDAVEECMAGYIRSRQRQDAPATGCHRKFSIRF"
     gene            20231..20425
                     /gene="rpmI"
                     /locus_tag="P5316_02120"
     CDS             20231..20425
                     /gene="rpmI"
                     /locus_tag="P5316_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRR83782.1"
                     /translation="MPKMKRHKGLDKRVKVTARGKVKRRKAFAGHLLSGRSGRRLRRL
                     KKAALMPKADARKVLFVLGK"
     gene            20498..20845
                     /gene="rplT"
                     /locus_tag="P5316_02125"
     CDS             20498..20845
                     /gene="rplT"
                     /locus_tag="P5316_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRR83783.1"
                     /translation="MPRATCGAARARRKRRLRAATKGYRAARSKLTRTMKNALTRAGQ
                     YAFRDRRARKREFRSLWVTRLTAACRARGISYSRFMSGLKKANVILNRKMLSELAIHD
                     QAAFDKIVALAKA"
     gene            complement(20903..22063)
                     /locus_tag="P5316_02130"
     CDS             complement(20903..22063)
                     /locus_tag="P5316_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR83784.1"
                     /translation="MNVCHVITRLIIGGAQENTLLTCEGLTRRGHKVTLAVGPDTGPE
                     GSLFEETRARGYDVRIIPAMHRAVRPFADLAACRQLWTLFDKLCPDVVHTHSSKAGIL
                     GRWAAHRVGVPIIVHTIHGMSFNRTQPAPIRSLYRFLERRCARYTDALIGVAEAMIRQ
                     SVEAGIASPDRFVTIYSGMRTEWYDPARHDRQAIRQQWGFGEDCVVFGTVARLFRNKG
                     YEQLIPAMAEASRRCPSLRFVWVGDGSQRSEYESQLLRLGLTNRVRLTGLVPPLEVAR
                     LLAGMDAVVHASQWEGLPRVVVQGLIMSKPVISFDIDGAPEVVLPDQTGLLVPLNDIQ
                     GLAEAMVRLAGDAAMRDRMGRAGREWCLRRFDHERMVDDIESLYKKLADRRR"
     gene            complement(22060..22881)
                     /gene="truA"
                     /locus_tag="P5316_02135"
     CDS             complement(22060..22881)
                     /gene="truA"
                     /locus_tag="P5316_02135"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRR83785.1"
                     /translation="MERNIRFLIAYDGTDFHGWQKQPEMRTVQDVVEQATRRVVRHQV
                     GLNAAGRTDAGVHAAGQQANFETTCEIPLINLRRAIGSRLPKDVSLLHVSEVPLGFRC
                     SQDAVSKLYRYRIYNATRRPVQEHLQRYTYHFWNELDTMRMQEAANRLVGTHDFAGFA
                     SKGSERETTVRTILQASVSRRYDEILIDVEGTGFLYNQVRNMVGTLLEIGRGRWAPQR
                     IDEILAARDRRLAGPTAPARGLCLQWVKYDLTRPPGWKGLGITSVPDEPAAAENS"
     gene            complement(22892..23674)
                     /locus_tag="P5316_02140"
     CDS             complement(22892..23674)
                     /locus_tag="P5316_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR83786.1"
                     /translation="MADKKDDIRALWNRYFKTRSDEARNKVVEYYAPLVHIQAARLSR
                     KLPAQIGYDEICSAGYDGLIEAVEAYNPTRKAKFETFCQQRIVGAVMDWLRSLDPQSR
                     TVRTFEKQRMMVRELLDAELNRPPMHHEIAKRMGLSQDRYDQLSRISQLGREVHFSAM
                     DPKDSSSRSRSSERNWDIGDRRQTDPAARMARQMLTNYLTRGLSREERLVLILYYYED
                     LTMAEIGVVLDLSESRVSQIHKDVITRLRNRFKGKLEEELVA"
     gene            complement(24824..27244)
                     /locus_tag="P5316_02145"
     CDS             complement(24824..27244)
                     /locus_tag="P5316_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83787.1"
                     /translation="MNRSQVANRTTQARVTRQTPADASPRRLICQIALAAILSGGVPT
                     GRAAQGEPVLHAGTSPKPIEASHFPDRLHAFVWRNWESADLSEMARVLGTTEDSVREI
                     GESMGLPQHVPITDIQKRRGYIGLIRRNWHLLNYDQLLLLLGWKAEKLAYTLKEDDFL
                     WHKLGALKPDCPPLRYEPPTEATQKRCAQIKELVTREFGESFGQPAQPRFAFVEELSK
                     PLSEMPPGPQAGDEPIRFIYSYFAVFGDPLLDARLNPYPDGLLQRLVGLGVNGVWLHV
                     VLRDLAPSQDFPEFGEGHETRLANLRQLVQRAGAHGVKVYLYMNEPRWMPSTFFEDRA
                     DIAGVREGDNTAMCTSTDRVRRFLVDSLAHVFREVPDLGGVFTITASENLTHCASHYQ
                     QASCPRCSKRSPAEIIADVNAAIAEGVRKGNPNATIICWDWGWKNEWAEAIIRRLPDD
                     VYLMSVSEWDLPIERGGVKTIVGEYSISSVGPGPRAQLHWKWAQQRGLKTMAKVQVNC
                     TWELSAIPYLPAMDLVARHIENLRDLGIRDMMLSWSLGGYPSPNLQLVREFSKTPPPT
                     AEQALARVAEDLYGKTAAPEVRKAWAAFSTAFEEYPYGMPGLYAGPFQLGPANPLYPA
                     PTGYRPTMVGFPYDGLDAWRSSYPADVYVSQCEKVARGFATGIELLRQAQTKADTDTF
                     SRTIAGELRLAEAARIHFMSVANQARFILNRDALSKPRPPIHTSTPQIAEVAQLTTTQ
                     RSVIEEEMRLAARMYDLTRADARIGYEASNHYYYLPLDLIEKVINCQHLLDSDKMKRV
                     TIGQDNPE"
     gene            complement(27322..28563)
                     /locus_tag="P5316_02150"
     CDS             complement(27322..28563)
                     /locus_tag="P5316_02150"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRR83788.1"
                     /translation="MADPSIDDSQLGRLAVDSKLLTLKELHDCLAEQKNLAKEGKRVS
                     LSEAMLRCGFVTRSQLQRLGAEMEDSIARKAQQIPGFQILGKLGQGAMATVFKARQLS
                     LDRIVAIKVLPKKLGENSEFVERFYREGRAAARLNHNNIVQAIDVGEASGYHYFVMEY
                     IDGKTVYDDLAAGKIYKESEALDIIIQIAKALEHAAERGFVHRDVKPKNIMLTRHGVA
                     KLADMGLARETGDLKAAMAEAGRAYGTPYYISPEQIRGEVNIDFRADIYSLGATFYHM
                     VTGKLPFEGSTPAAVMHKHLKEPLVPPDHIVPSLSTGVGEVIERMMAKKPEDRYASIR
                     DLLTDLEAIARGEPPLQARKKYDAHLLEGLAESAEPVEEKPAVQQSSEPKPLVVPVVW
                     VVVLAALLGVSLIVNIILAVL"
     gene            complement(28735..29979)
                     /locus_tag="P5316_02155"
     CDS             complement(28735..29979)
                     /locus_tag="P5316_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRR83789.1"
                     /translation="MMLHDPATDMSETDALKAACLTPPAMGGVAVIEVVGRHALRVVA
                     KYLRSRRPIDPNAVDTNEVRLCRWADGDQVIDDALVTVRRAPDGPFVVDISLHGGPRI
                     VQRALLMLQQAGVRIVEPTALLDDSCPAATVVEREILPLLLRAKTRAVAIWLAELVHR
                     LPKRIEAVLDLIEVGEATLARRELAALCQSVDQARYLLSGVRVVVIGPPNTGKSTLIN
                     RLASREQAVVSDQPGTTRDWVEHPGVIDGIPLVFVDTAGIRDTADPIEQEAIRRTYQQ
                     ASTADILLSVSDLTTPLETGLPPKKMTGDQSGMIRVPPIVLVANKSDLPAHPSWQNVQ
                     FSEAGFLQVSALASAGLEDLEARLVDSTGLTGWRQKLIAPVSPHQVACCRGALSALES
                     GRADPPAASRWLRDMLGPASPR"
     gene            complement(30141..31319)
                     /locus_tag="P5316_02160"
     CDS             complement(30141..31319)
                     /locus_tag="P5316_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRR83790.1"
                     /translation="MRQRSPSPDPQLLELIDNGLSREASDLHLVTGHPPTLRIHGDLF
                     PADKIPLSTEAAARIISSIMPAELRSRLDGQKDFDFSLSVGRPAGVVRFRVNVFFGQG
                     SMGACLRFVPSSIPTFEWMGIPQDLVERIVNLHSGLVLITGITGSGKTTTLAGLVEEM
                     NRLGHRRIITIEEPIEYVFLPKANTVITQREVGIDVASFADGLRSALRQDPDVILCGE
                     IRDVETARMAISAAETGHLVMSTVHTQDAKGAITRLIDIFPLERQQDIRCQLSLSLRL
                     VISQYLLPSVTAGQKRVLAMEVLTASDPARAAIRLNKIESLETIIQTGKKWGMQTLDE
                     NLASLVCNRRISPEIAHRYAKNPAGLRAMGVPDPPNSSFENAAGNVTRSSGRADVLSP
                     "
     gene            complement(31515..32336)
                     /locus_tag="P5316_02165"
     CDS             complement(31515..32336)
                     /locus_tag="P5316_02165"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRR83791.1"
                     /translation="MIAESGRSGKAHLILMDIGNTNVAMASWFDDHRDEAEHIPTVSF
                     DQLMNRLERLWNELPGTATRAVVICSVCPPVLAKLRQACAAKGIKPLLVVGEEIESPI
                     PLDLALREPSRVGTDRLCAAAGAFARVRGACVVADFGTALTIDLVADNGIFLGGTILP
                     GVALSARALHEHTAQLPLVEVGCPTETLGKDTQSAIRNGIYAMMVGALREIAERYATD
                     IGKWPPLVITGGNAPDIARGADFVDKVMPDLCLDGLVIAYKQHAGTDEDQPGVCR"
     gene            complement(32333..33544)
                     /gene="obgE"
                     /locus_tag="P5316_02170"
     CDS             complement(32333..33544)
                     /gene="obgE"
                     /locus_tag="P5316_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRR83792.1"
                     /translation="MFIDRAEIYVRGGNGGHGCMSFRRERFIPKGGPDGGDGGRGGSV
                     YLVADESVATLLDMAGHHHWVAENGKPGKGKNMTGRSGKDLVIRLPPGTLVYDRDNGR
                     LLKDLDAPGKKVRIVRGGRGGRGNAHFATARHQAPRYCQPGEEGQERRLRLELKLIAD
                     VGLVGLPNAGKSTLLSRLTKARPKIADYPFTTLEPQLGIVEMPGYQRFVMADIPGLIE
                     GAHEGHGLGDDFLRHIERTRMILHLIDVHPLPGQARPIEAYRIIRNELRKYSEALAAK
                     PELIVANKIDLAENNDAVDQLRDELEALGIAAEEVIAISGVTGSGLERLGYRLWEMVE
                     AAKARERVAASALAVKSKDDPFRGILPDGEEPDEADFEELLSTNEESSEAAQGDFEDN
                     ETADDLTGGAS"
     gene            complement(34066..34305)
                     /gene="rpmA"
                     /locus_tag="P5316_02175"
     CDS             complement(34066..34305)
                     /gene="rpmA"
                     /locus_tag="P5316_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRR83793.1"
                     /translation="MAHKKGQGSTRNGRDSNPQYLGVKLYGGQQAKVGSIIVRQRGTV
                     FKPGRFVGRGKDDTLFALVDGTVVFKGRRVDIAPN"
     gene            complement(34348..34965)
                     /locus_tag="P5316_02180"
     CDS             complement(34348..34965)
                     /locus_tag="P5316_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83794.1"
                     /translation="MLSAATSQPAPVAIQPSAAPVAWELNFRFEDPQRVSVVVPGKDE
                     PVVYWFMLYRVDNPSDQEVDFYPAFELVTNTLQVIESEVRVSPEAFRAIERRANDPLL
                     VAPERAIGRLLRGKDRARHSVAIFRDFDPKATGFTIFVAGLSGEVRREKNPAFDASKP
                     ESETNKRYFTFRKTLAIPYKFPGSPVTRRSVIPERLADKQQWIMR"
     gene            complement(35483..36793)
                     /locus_tag="P5316_02185"
     CDS             complement(35483..36793)
                     /locus_tag="P5316_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83795.1"
                     /translation="MSKRQTRREFLKASALAGAGFWVSGRQTLGQTTSPSEKLGVAVI
                     GVSGQGHWNLRQVTGPEAAEICNIVALCDVDENKAAEVRQRFPKARFYTDFRKMFDQK
                     DIDAVVIATPDHTHAAAALPALRLGKHVYCEKPLAHTVREVQLMREAAARAKVATQMG
                     TQIHARNNYRRVVEVIQSGGIGAVHEVHVWVEREWGGQERPTETPPVPEGLHYDLWLG
                     PAPYRPYHPAYLPANWRGWWDFGGGTLADMACHHMDLPHWALDLRHPTTIEAEGPPVH
                     PESCPVWLIVRFHYPARGKMPPVLLTWYNGKRRPYYFQEGKLPEWGDGVLFVGDKGML
                     LADYDRHVLLPEKDFADYKRPEPCIPESIGHHLEWLEACKSGEKTSCNFDYSGALAQT
                     VLLGNVAYRSGKKIRWDAERQRITNTREADTFVDPPRRKGWELA"
     gene            37203..37757
                     /locus_tag="P5316_02190"
     CDS             37203..37757
                     /locus_tag="P5316_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83796.1"
                     /translation="MACFTSVGGKGRAGRWFQGAAIPLLCGVAMVMALSAAGCVVDSS
                     HPPETLSGEQTIEEAETSDGTASKSVESRWTAPDQNFVLSVEVTSPEPLGVENAQLQV
                     TLTCQLGTTKTFPVTLTPDGSSKLVASFSRAADAPDGSECVGIGGQKEAAKWKVTLSR
                     PADTTQALTFKYRMDYRLASIETP"
     gene            complement(38136..38636)
                     /locus_tag="P5316_02195"
     CDS             complement(38136..38636)
                     /locus_tag="P5316_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HRR83797.1"
                     /translation="MLAEIYWIKPLPYRLAIMPRPRGGEWLGDEIASLRRQGVDVIVS
                     LLTPDEIAELGLEQETECCHSAGIEFLSLPIEDRSVPADPRATRDLIERLAVDLRGGK
                     AVAIHCRAGIGRSALVAACILAKLGVTTTDAFRAIGQARHCPVPDTPEQIDWADKSAL
                     GLETEM"
BASE COUNT         7533 a        11793 c        12056 g         7458 t
ORIGIN      
        1 agtctccagt gtcacgatcg ccctattgcc cagaacgcgc cgatagccgt cggcatagcc
       61 caatgaaacg gtgccgatca cgctctctcg cttggccctc cacgtgcagc cgtaacctac
      121 cgaagcaccg gcaggcaatc ggcggatttc cgtcagacgc acgaccagcc gcaagatcgg
      181 gcggagatca ggcggccttt cactggccgg tccgccccaa tacccgtaga tgcacaaacc
      241 cggtctgacc aggtccagat gtgattcggg caggcggaaa acggcggccg agttggcggc
      301 gtgaaaggac ccgaccgcca gcttcgcctc acgcatgcgg gcgatgagct gcttgaattc
      361 ttgaagctgg cttcgcgcaa acctcaggtc atcctcatcc gcggtggcaa agtgcatgta
      421 gacgccgtcg atgagaatgc cgggcaccag cgcggtctga gcaataatgt ccggtgcctt
      481 gtcacatcgg acaccgcatc ggcccatgcc ggtgtcgacc tggatctcga catgccccat
      541 cctgtgttgg cgcttggcag ccgtggcgag caggcgggcc tcctcgggag aggtgatcgt
      601 caccgcaata tgggccgcca cggcttcggc ggcgttctcg attcgttcgc tttcgcttcg
      661 gctggccaat atgggtccga gacaaagaat cggaccagtc caaccaagag cgcgaagctc
      721 gacggcctcg tcgagattcg cgaccgccaa gcgctcaatg ccggcacgga caagggccgg
      781 cagcacctgc gagagcccgt gaccgtaagc gttcgccttg acggcgacgc aaatgggtgt
      841 gccgggcggg atgcgtcgcc ggacggcctc cacattgccc tggatggcgg cggcgctgac
      901 gtaggcagtc gctggctcat gcatgggtcg atcaggtctc ctgcgccaat cttgaagggc
      961 ggcagcccgc gccatctggc tccgccgacc ggcatggtat acaatctccc ggcgattggt
     1021 acaaactcaa tgattctttg ggatccatgg cgacagcgtt cgcctgcctc gaaatgcgca
     1081 aacaccacga accgcccgca gcattcgccg aactcggcat ccaaacacca ttcctgtcag
     1141 ccctgcaaag aatgggcttc agccggccca cggagattca gcaacggctt atcccgttgg
     1201 cgttggccgg gcgagacgtc ctcggtcaag cccgcacggg cacggggaag accgccgctt
     1261 tcggcctgcc tttgcttcaa ttggccaagc cggccgaggg gctccagggg ctcgttctcg
     1321 tgccgacccg cgaactcgcg gtgcaggttg cggccgagat gctccgcctg gccgagacct
     1381 caggcttacg cgtcgtaccc gtctacggtg gacagcgaat ccgtcagcag gttcacctcc
     1441 tgggccgcaa gccgcatttt gtggttggca ccccgggccg cgtgatggac ttcctggatc
     1501 gccgaatcct ctcgttcagc ggcattcgtt ttgtcgtgtt ggacgaagtc gaccggatgc
     1561 tggacatcgg tttccgcgac gatatcaagc ggatcctctc gcaggtcaaa accgcccacc
     1621 agaccatcct cgtctcggcc accatgaccg atgagatcaa acgccttgcc gagcagtaca
     1681 tgaccgaccc ggtcgaggtc aacgtctccc aggacaagat gaccgtggat gaggtgcggc
     1741 agtccttcta cacggtggac cgctgggaca agtaccggct cttgagaacc gtgctcgatt
     1801 ccaggaagcc caagctggcc atcgttttct gcaacaccaa gcacgcggtc cgcaagttgg
     1861 caaagcggct caacggcgac ggcatcaacg ccagggaaat ccacggcgat ctcgttcagg
     1921 ccaagcggga ccgggtgatg tcgcgattcc gaaagcatgc gatccacgtg ttggtcgcca
     1981 ccgacctggc ggcccgaggc atcgacgttc agggcataac ccacatcatc aattatgaca
     2041 ttcccgatga cccgctcggc tatgttcacc gcatcggccg cacggctcgc atgggctgct
     2101 tcggagaggc tttgaccttt gtgacccgcg aagagggcaa acagctcacc gagatcgaga
     2161 agctgatcaa cagggagatt ccctgcgagc aaatggaagg cttcgagccg tcgccgcccc
     2221 cggagcagca ctccgctttc gccatcgccg ccaagccgga ggcgtcggtc gacgcgccgg
     2281 cagcaaatgt ctcgacggcc cctaaacgcc tgggagccaa gttcaggccc gtgcgccgca
     2341 gacgattgtg agcaaggcac cgccccgtga caatgcagac acgcaccgcc cgcagcttgt
     2401 catggtcgtg tcttccgcgg atcaggcggg cagtcgcctt gcaaccttcc ccgggcacac
     2461 ctatactggc cgatgagatg acttgcccgc aatggagcga ctatgccgat ctacgagtat
     2521 cgatgtctga aatgccggca cgagttcggc cggctggtcc ggtctgacgc ggaggagcag
     2581 gctctggtct gtcccaaatg cggaaagaaa gagctggaga agaagctgag cgttttcgcg
     2641 gccgggaagg gggcagcgtc cgagccctct ttgccgccgt cctgtgccgg ctgccagaat
     2701 cttggcggcc cgtgcccgat gaggcagtga aagctcgcgg ttgaaagcat tgtcgaatga
     2761 agactagcct tgccgaagac aaccctcaac ttcgcatgtc ggttgccgct gtattgactt
     2821 ggctcatgcc gggtgcgggt catctgtata tccgtgagcg ggcaaggggc cttattattc
     2881 tggtgaccat cgcgatcacc ttctgggtag gcgttgccgt cggcggcctt agcaacacgg
     2941 tttcgcccgg cggccggtcg ttatggttca tgggtcagat ctgtgccggc ggccacgcct
     3001 tggccgcctg gggcttgggt acctgggtgg atcccagccc cgtgcccaag agggatccca
     3061 agttcgtggc cttcggccga tccgaagaga tttcggtcgt ctacacggcc atctgcggca
     3121 tgctcaacat cctggtgatc ttcgacgtgc tggtccgggc ggaaaagccc ctgatccagg
     3181 cgggtcgcac cccgcctgcg gcaaaggggg cggccggatg atcgagctgc tcgccactct
     3241 catgatcaac cccctccctc tggttcgctg gcagcaggcc ctgttgttgc tgccgctctg
     3301 cctctgtatc agcatcatct acaagaccat caagtgcgag aacctccggg agatccccat
     3361 ggcggtcctc aagaactgga tcaccatcgt gatcggaatg tatgtggtcg gcatcgccct
     3421 tctgttgatc tacgaattgc tggcataaga ctggccgggc gcaagcccgg gcccggaaac
     3481 gcggtgttca aagcaccggg cgaggcgagc acgtcttgca tcccattact gagtctgcgg
     3541 tgttgtcaat tggtcgactg tgagatggcg caggcggcgg aggaaatctt cgatggtccc
     3601 ccggtgctga gacgtgagcg cgtgaccccc gaatgcgacg tcatagtagc attgcacgag
     3661 aaccggggcc tcttggagaa tggcgtaacg ttccgccaga tcggcggcga actcggcggg
     3721 cgtctgatcg ggcctgcgag tcagtccgag atccgcgagg aagcggcaga atcgatggta
     3781 gaactcgacc tcgcggctgc ggcttgcctg cctggcgtgc cggccctgcg agagacgccg
     3841 ggcaagcctc gtcaatcgac gcgccaagga gacggcgaca taggtcaata atagaaccag
     3901 cgtcaccacc aggagcgtga aaacccagta aaccaggcgt tcgtgccacg acagagcaag
     3961 acgccagccg aacaactcgc gaatgaatgc gaccacgccg ccgatcagcg ttcgctggtc
     4021 ttgagccggt ctgcgaagcc attgggcgaa ccgatcgagc attcggcgcc gcgtttcggc
     4081 gttgaaagag agaactccgt cggaccactg gaactggagg aagtccagat aagcgcggat
     4141 tcgggcgccc agacccgtca gccgccccga ttgccttcgg gacaacggcg tgggatccag
     4201 cgtcacccaa tcgaggccgg ggatgaacac ctcgacccat gcgtgtgcgt gcttcttgcg
     4261 aatcaggtag aagccgccga acgagttgta ctcattcgcc agataaccga tgaccaccct
     4321 ggcagggacg cccttgagct ggcacatcag ggccaatgcc gaggcgaaat actcgcaatg
     4381 accctttcgg gagaccagca aaaaactgct caccgggtct ccggtctcag gcatcggcgg
     4441 aggctcgagg gagtaggcaa aatgctcgga ttgcaggaaa gcctccagcc ggcgggcgaa
     4501 ggataagcgg tttccaggct ctgtgagcgg gccgattccg cgcgtctgtt catcgatgag
     4561 acggagaagc tgctctcgcc gttcgttcgc gatcggggga ggcgtcggcg gggggacgtc
     4621 ggcggcgccc cgttcggctg ccagcgcttt ggcggccatt gccggaaccg ccggggactt
     4681 gatcgtgtag tccagttgct tccgcaccgg cctgagagtt tgaagaatct gtgtcccgtt
     4741 ccattttcgc acggtccgca aggtgggcga gctgatctcg agcggtggat aacaggcgaa
     4801 cagcgtgatg tcgtcggacg gctccaggcg gtacttctgt atgagcatcg gcccgtgcgg
     4861 cagagcgatg tcattgcgga acagggtggt ggagggcgtt gtctcttcat ccgaagtgag
     4921 ctggtaggtg tcagcgctga gctccgtcac tcccgatcgc cgccagcccc agttgcggtt
     4981 tcctgtcggc gatcggctgg tgtaccggtc gaggacttct ccccttagat aaagcggaac
     5041 cctcgatccg acaggcgtgc catattcgtc cgtcgccgtc agacgcatga tcggctggtc
     5101 cgattcctcg atcgcgccga ccgaggcgaa ttcgatcgat cgggcgaacc cgctgagggc
     5161 gccggccgtg gccggttgcc cagcctggcc gaacatgccg gcaccgacgc ggggtattgc
     5221 gacgaagacc atgaccccga gtcccagggt tacaatggcg acggagagcg agccggtgcg
     5281 ttgtgcattc aggccggcgt gcgaggtcgt tccgtcgggc cggcgaaagc ccgatgcctg
     5341 aacattgcgc cgatcggacc aggcggcttc gctttcaagg tgatgggtga tgaagcgctg
     5401 acacccggcg atggcataca cggccaacac cagcgggaaa agcaggctgc caccgacaat
     5461 gccgccgacg accagcagca acaggagaag tatgagcagc atggcatcgt cgcgatgggt
     5521 gcgccattgc agccacttgg taatacagat gagaatcatg aaatgtgcaa gagccaggac
     5581 cgtaggcacg gagaaacgcc agccccactc gatgatcagg aagacaaaag cgacgacgcc
     5641 gagaaccagg gccgtatggg ccgagaggag ggggcgtccg cggtacagca ggctccagag
     5701 gccggcgaca ccgcttgccg gcgtcagaat cacgtacaac cagaagttct cggggtgcgc
     5761 cgatcgctcg gcacttgcca gcaggacaac cacgccgatc agcatggccc cttccgagca
     5821 gaatcgtcgg atcatcgcgt tcatcgacgc ctcctgacgg gagcgcctgg atgggtcagg
     5881 tcgaacagca agtcgaaagg ttcgctgtcg ggggacaaaa ccaggatcgt ttcagccctg
     5941 gttccgatga ttctggcgac gcgctgatgg acgctccgta tgtgactgct cagcaacagg
     6001 cagcgagcgt tccagcccgt ggaccaatgc acgcgtccca ccagttcgtc gaagctcgtt
     6061 tgggcgccgg gacctatgcc ggcgagctcc gtgagcaggc gttgtcgatg gaccttgccg
     6121 gccgtcgggg cgatgaccac gggcttggcc gcccggcaga tcagacctac ccggtgtccc
     6181 tgttccaggg ccgcacaggc ggctgtggct gcggcgctga tgagtcgctc ggcccgtccg
     6241 tcatggtcgt ccgtgtcttg ctgggggcgc gacgtgttcc ccgccgcgtc cggccaggga
     6301 tccaggatga tgatcatccg agtctggcgc ggtgatagca tttccctcac gaccagttca
     6361 ccagtacggg ccgaacgtcg ccaatgaatc cagtggtagt tgtcaccctc gcgatactcg
     6421 cgaacgccga caaattcgtc tgggttctca cgttcccggg ctgtgcgggc tgactgagac
     6481 tggcctcctg cggtcttttt ccataggtca tggcgcattc ggcccatggc gggatacacg
     6541 gtgaggctgc ccgctgaggg ggcttccagc acgcatgcga aaagaccgaa cggataaccg
     6601 caggagaccc ggatgccggt cagaaccagg tgtgcgcgcc gggggcattg gccgagtgcc
     6661 tctacacgtt tctcctgtcg agggggcaga cattcgacga aaacggtcgg gaatcgtgcc
     6721 ggccggtcat tgacccgcat ctcggtgacc gtcaggcccc gagcgtgcag ccgtttgcgg
     6781 cggtttctga gccgataagc cacggcgaag gggcggtcgg ccacgacgcc ttcgggcgcg
     6841 attcggtcca cctcgattcg gcgaagcatg aggatgggga ataccgcgtt caccagcaaa
     6901 gcgcccactc cgattccgaa gagcagaacg agcaggtttg cctcgctgtt gaggcccgcc
     6961 agcagtacga caacgttcac aaaagcgaag agcagaccac cgaaggtaaa atgaagcgtt
     7021 cttcgggcag tctgggcgcg cggggcgtcg agacgcttgt gctgcggttc gaggagaatg
     7081 cacctgtcgg ccaaggcggt catctgattg tccccgtcgg cgcgcggccg tggcgcgtgc
     7141 ccattattca ggcacggtca cggtgcggag gatctcggtc aggatctgtt cggcgctgat
     7201 tgtatgtccc tcgtgcacga agctcttcgt cgcgatgcgg tgcgcgcaga cgggaatgaa
     7261 cagggacttg atgtcatcgg gaatgacata gtcccggccg gcgagcatgg cggcggcttg
     7321 tgcggctcga ataaaggcca gggttccgcg aggcgagaca cccagttcca ggtgctcgtg
     7381 ctcgcgggtg atttcaagca ggtccagaac gtactggacc aaccggtcgt cgatgcggat
     7441 ctggggcacg cggtcctgaa tcgcaatcag gtcctcgcgg cgcatcacgg gtcgcagaac
     7501 ctccagggcc gtcttgctcg ggtcctcgcg gatgatacgg gcttcgactt cacggctggg
     7561 gtagccgatg cggattctga gagcgaaacg gtcgagctgg ttctcgggca gaaaataggt
     7621 gccctcgaac tcgaacggat tctgcgtcgc gatgaccagg aaaggctgct cgagtttcag
     7681 ggtccgaccg tcgacgctga cctgagcctc gttcatggcc tccagcaggg ccgactgcgt
     7741 gcggggagtc gtgcggttaa tctcgtcggc cagaataatg tgggcgaaaa taggcccgcg
     7801 cttgaactcg aaggtgccgg tcttcgcatc gtagatgctg accccgatga tgtccgacgg
     7861 gagcaggtcg ggggtgagct ggattcggga gaaggacagg tccaggctgc gagccaaggc
     7921 cctggccagc acggtcttgc ccacacccgg tgcgtcctcg atcagcaggt gcccccgact
     7981 catcaggcca accagaacct gggtgaccgt attgggcttc cccaggaaca cccgatcaat
     8041 gttgtttcgc agctcggcca ccttggccag gagaggatcg cctgccagtt gaaggttcgc
     8101 ttccataaat actgaccctt tgacagagag gcccttggtc tgatgcccgc ctgattataa
     8161 gacaatcgcc ccgaaaaccc tacgacttga aggtggggta agttcgaaac ctgatttccg
     8221 ggtgccaggg cggctggggc agggtacaat aagtcggaaa cagcacttgc gggaggggga
     8281 agtctgcccc gtctcggagg gagatcacat gatgcatgga ctcaagacgt ggatgccagt
     8341 gctgctggcc gtcggatgga tcgtcgtcag cagtggtgcg ctgctggcgg ccgaggatac
     8401 gagttcggcc ggcagcaagg tcaacattcg cacctcgggt ggaatgagaa tcgtggcggt
     8461 cagacgcccg gcgtcgtcac ctgccatcaa tactcaagct ctcgctgccc cctcatcagt
     8521 tgtgactgca gccgagccca tgacggctgg tacgctgtcc acgccgtttc cccggccgcc
     8581 tctggcgggt ttttctcctc tcgtggccat aacaacctcg gataagcggg aattcgatga
     8641 tctgatgtgg gatcacactg tgcaccatac ctacacaggc aacgccctga atgcgcctgc
     8701 gaacgcgaac tttatcatcg gcatcttcga cagcggctca gtcgttgact tggtagctga
     8761 gccgtgggac tggatcgtgg gcctcaccgg aacgcgtctg acggggaacc cctttccgat
     8821 cggcggtacg ggagggaccg tcgatgccat catctccaag cccgttggtt attttgcgaa
     8881 cgggttgagc gctgtcgacg cagcctcagg tctgcttgac ctgaccaaag tcgtcggtca
     8941 cggcaatacc tgcctgctgg tagcacctga gattgcgtgt ggtaacggcg aggggctgac
     9001 cgccgtgttg ggaacaggtt ttgtgtcatt cttcaatacc attattcgag tggacacccc
     9061 gatacgtcgt ggttttggcg gcgacgacgt gctcagcccg agtgtccaga ttcaggatca
     9121 gtcgatcgcc ctgccgctgt atcctcggaa gatgtcgatg accttcagct cgggcgggct
     9181 tccggtgaca acggcctcat actacggcct gttggatccc tggacgtggg aggtggatat
     9241 catcctgccg acgctcttgg gtatgggtag cggcatgatt ccgctaggtg ggtatttctt
     9301 tgggacgatc ggggcgatcg aaggagaacc ggggccgacc aatcccttac agaacatgcg
     9361 agtgctcgtc gacacgggtg cccaaagttc gatcatcacg ccaggcatgg ccgccaatct
     9421 caaccttgac ctgtataatc ccgacttcac ggtcgacata tgcggaatcg gcggcctgac
     9481 taccgacgtg ccgggattct gggtcgatta cgtcaagatg aacgccgccg gcggtgcatt
     9541 ggagttctct caggccccgt ttgtggttgt ggacatggcc tcgcctgatg gagggcagtt
     9601 tgacggtgtg ctgggcatga actttttctg gaatcgcaac gtgatttttc agccggtacc
     9661 gtctggcagc agtttcctgc acatctcgga ccccattccc ttcgcttacg gcgaccttga
     9721 ccgcgacctc gacgtcgatc aggacgattt cgagatcttc tcggcgtgca gcagcggccc
     9781 cgggatcccg ttcagcgccg agtgcacgct tgtcgacgcc gacgatgatg gcgatatcga
     9841 ccagacggac ttcggcttct tccaggcctg tctcagcggt gagaacgttc cggccgacgt
     9901 aaactgcaag ccttgattca gaaagcgttc agcatgtttg ttcagagaca gcttctcctc
     9961 gtgctcacgg tacttgttgg ccttgctttg atcgccgctg taccatcgcc gcttcggccg
    10021 ggcgctgcct ttgcctcggc cggtgacgag gaaggtgttc aacaggagac gaccatggcc
    10081 ggcaagacgc ccaagcagaa gacaagaccc gcggccgcga aaaccggtgg tggagatgat
    10141 actgctgcgg gcaggcagtc caccaagccc gcgaaagccg acgccgggca gatccgcgtt
    10201 gcgatctgcc agatcctgtg tattgacggt gatgcggagg gcaacttccg gcgaatcgaa
    10261 aacgccctgg aagtcgccgc ggccgccaag gcacaactgg cgtgctttcc ggagtctgcg
    10321 atactgggct gggagaaccc cgatgccttc aaactggccg gcccgattcc ggggccacat
    10381 tccgatcgag tcgccgccct ggcccgcaaa tacaagatga tgatttcgat cggcattgat
    10441 gaactggcgg agcccgggtg cctgtatgac tcagccattc tggtggatga gaagggcaag
    10501 attctgctca agcatcgcaa actgaacgtt ttgcctgagc tcatggaccc gccgtactcg
    10561 gtgggcaagc cggaggatat caaggccgtg gatacgccgc tcgggcggat tggcatgctc
    10621 atttgcgcgg ataccttcac cgaggagtac gtcaagcggg ccggctcact caagccggac
    10681 gtgctgctgg tcccgtacgg ctgggcggcg ccgaaggaaa agtggcccgg gcacgccaaa
    10741 aacctccaca aaacagtggc ccaagcggct caatgggccg gctgcccagt cgtggggacg
    10801 gacctggtgg ggatgatcac ccacgggccg tggaccggac aaacgtacgg agctgccagc
    10861 gtcgtcgcgg accgccaggg caacattctg gtgattctcc gcgaccggga tgtggacgtg
    10921 caggtcattg atctggcgat tggccgtcgc tgaacagcat cagcgggtcg tgcctgatct
    10981 gtgcaaatgc agccgaacta aggtcaatgc gctccagcgt cgcatacggc aacgctggcc
    11041 aatgaagaat ggcaagtgcg gaattgagaa tggagcttgc tctgtcgcgc acgccgagat
    11101 gcacaggcgg tggtgtttcg gtgcaaggca agaaatccga gaacaaggcc gtttccaggg
    11161 ctccgtgcga ttgtcccttg ttcggattct atgaaattga ggggtacagg ttgtgatctt
    11221 gtttgactgt gccagtaagc agcggcttgc ggaacaaaca gcccaaggcg gaccgccttt
    11281 ttgcgggccg caacccaagc gctctgccat ccagaaagat gcgcgcaagc cgcacagatg
    11341 cctggccgtc aggttctaac ggactaacag ccgaacagac tgacagaccg ctggagatgc
    11401 tcccttgcca ggctggttta ttacaggaac ggatacaggc gttggcaaga cggtgattgc
    11461 cggcgcactg gcgatgctgc tccgcgagca caaccgcaag gtggctgtgt tcaaaccggt
    11521 tgccacgggg tgccgaagag acgtgcggct cgggctggcc agtgaagatg ctgaattcct
    11581 tgctcattgt gccgagagcg acgcgactct tgagaccatc aatcccgtgc ggtacggggg
    11641 ggatgtggct ccgatgattg cggccgagca cacccgcaag ccggtcgatt gggaggcgat
    11701 tgaccaaagc tggtgtcggc tgcgggcttc tgccgattgg gttctggtcg aaggggccgg
    11761 agggcttctg gtccccgtcg acccgaaaaa caacatggct gacttggcca agcggttcaa
    11821 cctgccgctg attattgtag cccggccggg tctggggacg atcaaccaca cgttgttgac
    11881 catcgaggcc gcccggacgc gaggactgcc catctcgggc gtggtgatta acggttaccg
    11941 gcccgactcc gccaccttgg ccgaggaaac taatcctgaa gtcatcgccc ggctggctaa
    12001 gatttcgatg cccttgatag cgccttttga tgctcgaacc gaccttcggc aagggcttgt
    12061 gggcgagacg ctgcttcatg ccctgcggcc ctttgtggaa cgcgcattgg gatagcaggg
    12121 ctttgcctct ggtccgcgcc ggtgcaaatc tccatactcg ctgaacccaa gaagtgttcg
    12181 cgacgtatct ttttgtaaat taagcgttta agacccactg cgcccgcagg aacctgacag
    12241 gcactgtgtc tgcggacggg atggcgcagc acgcgctgcg gggtcgccgc gactgataac
    12301 tcacaacctc ggctgcctgg gatctggctt ttgcgggact gttcatgaga acttgacatg
    12361 ggtggcctgg ggtgctatct agtttggccc ccgccggacg cgccgagcat gctcgtccgc
    12421 agggcggcga accacggccg cgtgagtttc gtaatagcgc ggcgtaaacg gtgtgccgac
    12481 gggcggagac cctgcgcggg atcagaaata acgtcagaaa gattacagga aaggctgcgg
    12541 ccggggaact cgaagagctc cgtggtcgct tgtacattca aaggcaggaa cgagagcaaa
    12601 tcacatgatc gaggtgagaa atctgaccaa gttctacggc tcacacaagg cggtcgacaa
    12661 catctcgttc agcgtggaag agggcgagat tgtcggtttt ctggggccca acggggcggg
    12721 caagagcact acgcttcgca tactcacctg tttcctgccg gccacgagcg gctcggcgag
    12781 cgttgccggc ttcgacgtct tcacccaatc gctcgaggtc aggcgtcgcg tcggttacct
    12841 cccggaaagc aatcccctgt accaggaaat gcgggtgcgt gagttcctca acttccgcgg
    12901 taagctccgc ggaatggagc gaagcgcacg cgaggcggcg atccggcgcg tggccgatcg
    12961 ggtttggctg ggcgatttca tcgatcggcc gatcggtcag ttgtccaaag gcatgaagca
    13021 aagggtaggt ctggccgatg cactcctgca cgatccgaag gttctggtgc tggacgagcc
    13081 caccatcggc ctggacccga cgcagattcg cgagacgcga aagctgatct ccgacctggc
    13141 caggcggcat accatcattc tcagctctca tatcctgccg gaggtcgagg ccacgtgcca
    13201 gcggaccatc atcatcgccg gcggacgtat tcgggccagc ggctccatga gcgagatccg
    13261 ggatcgcatt aagggcgcct ctcgggtgat cgccgaaatc cgcgggccga aggaggacgt
    13321 ggcccgggcg atacggcagg tgaacggagt caaaaacgtg gagatcaacc cgaacgaagg
    13381 ctggaaccat cttcggatcg agacttccga gggacgtgac gtgcgcgagg agatcgcgtc
    13441 ggttgccgcc cgccataact ggggcctgcg cgatctgcgg ttggagacgg gcggtctgga
    13501 agagttcttt atccagatca ccgccgaggc ggccggctcg acggggcgat aacgctcgcg
    13561 ccgcctgact gttgcttcca tactgtttca ctgaggacgg gcatttatgt ctgagcaaaa
    13621 tgaagcgaag gtgccgagcg cggccggggc gaccagctcc gtggccgtgg tcgcgctgat
    13681 ggaacgtgag ctgtcggctt tgttctactc gccgatcgcc tacattgtcg gtttcgtgtt
    13741 cctggtgcta tcggggatgt atttcaccac cgacgtgctt cagccgggca acgaggcgag
    13801 catgcggttg ctgtttgagc gtatcgccgg cctcctggta tttgccctgc cgttgcttac
    13861 catgcgttcg gtggccgatg agttcgcctc aggatcgatc gagtccctga tgaccgcccc
    13921 ggtaaccgac gcgggggtga ttctcggcaa gttcttcggg gccctgatct tctatatcgg
    13981 cttgttggcc acgactgtgc cccacctgat cctgatgacg atttacgcca acccggtcgg
    14041 gacggagatc gtcagcggct acatcggtct gatcctgttg ggggcgatgt tcatctcggt
    14101 cggactgttc gccagcgcca ccacgcggca ccagcttctg gccgcgatca tcgccgtggc
    14161 ggttctttcg ctgttcagtt tcgcggcgga gtacgcgacc gcccaggtga ccaacgtctg
    14221 gctcagcaag ttcctcaagt cgttgaacat tctcgatcag ttctccaact gcaacaaggg
    14281 aatgatcgac tcggcgagcg tggtgttctt cgtgtcgaac acctgtctgt ttcttttcct
    14341 ggcaatcaag gttctggagt cgcggcgatg gcggtaaacg acaagcatca cgagatgagt
    14401 gccacgggcc ggcgcctgtt tttcggcacc aacgttttca tcgtcattct cctgctggcg
    14461 gcggtggtgg tcggcatcaa tttcctgttc accgacccga gactgaacaa acgatgggac
    14521 ctctcggccg gttcatcaag ctaccgcctc agccagcgga ccaaaaacgt cttgaagcag
    14581 atcgagggag acaaaattgc catcaccacg gtgtacacct cggtcgagcc cgagtacgaa
    14641 cgcaagacct atttccctcg cgtcgaggat ctctgtgacg agatcgctca gttcgataag
    14701 cgcgtgaggg ttacacactg ctacagtcca gcggacaagc tcgcgttgcg caaccgcatc
    14761 cagggcaagt tcggcggggc ggccgagaaa cacaacgagg tgatcgccgc cgccaggacc
    14821 ctttgggaag acgtgcagca atccctgccg ccgctccagt cgcagatcga gggcctccag
    14881 cgcgacgaca acgcctggct cggcggattc tcctcgctca accgcatcaa tctgaacctc
    14941 aaggccaatc tcgagaatat cgctgagacc aggcgagacg tccaggacct gactgaaggt
    15001 caggacgcca tgcccaagta ccaggaagcc accaacaaga tcaagcagac caacgaccag
    15061 ttgaagcaga cgctcgagga tgcccagaaa tggtgcaagg acatcaactc gttggcaacc
    15121 gtgctgtcga acaaggactc ggatttcgcg gccaagaccc gcaagcagct tgaggaactg
    15181 gacaagctcc tggccaacct cgtcgccggc gtcggcgacc cgagtgacac aacaattccg
    15241 gatgacccca agccggcgat ccaggacttc gccaaggcct ccaatgcgct cggccggtgg
    15301 ctcacggagg aagtcggaag ggtcaactcc ttcgtgaagg ccaatccggc catcgagcag
    15361 catccgcgat ggatgcttaa cgtgcaactt gggccgctgg tggccaagat gcccctgtcg
    15421 gccaacctga gtgagacggc ccagagcgtc agcgagaacg tgcaggccgt gcgacaggtt
    15481 ctctccgcgc cgaacgtacc caaggaccag cttcagagct tgttgaggca gctccgacag
    15541 atcgccagca acataggaca gaagctggac aaggaatgga agtccagcat cctcgcgatc
    15601 cttgacgacg gggcccgtat tgaccaggcc agcagggatt ttcttgcccg cggcagcgaa
    15661 ggcacgatgt tcaaggaaat cctggacaag atcgcggaca tcgccaagaa gatcgaaagc
    15721 ctgcccaaat tggagctgga tgagatcgcc gaacggctgc aggacgagaa cctcgtggtg
    15781 atcgaggtgg gggaccaggt ccgggtggtg aagtttgacg aggtctggcc catcgccgac
    15841 ccgacgatgc ggatgcggat ggacgagagc aggaccgccc gcgtgtttga cggcgacagc
    15901 gcgatttcag gcgccttgct ggcgatgacc cgccagaaga aatacgggac ggtgatcttt
    15961 gtggcctacg agaccgaggc cgaccccatg gcccgacgga tggggcaagg cggccgcaac
    16021 accggtcgaa tcccgctcga acagttgagc acgctgaagg acaaactcaa acaggcgaat
    16081 ttcgcggtga aggactggaa cctgggcgcc gaggggacgg acgccgagcg gccacaaccc
    16141 gaggaaggca ccgaggccat ctatgtcttc ctgccgcctg cggaaacgtc gcctcccaat
    16201 ccgatgatgg gtcggccgcc ggagaagggt ttcggagagg agcagcttga gaagatacgc
    16261 ccccttctga ccgcggggtc caaggcgatt ttcttggcca cgtgggtcat cccacgaatg
    16321 agttggtttg gcccggagcc ggtcggccct tatgcctacg gcgacatcct cagcaaaaac
    16381 tacggcgttg aagtacacca gtcttatcgg ttgcttcgag tgctgcccga cactcatgac
    16441 ccgacgctgt tcggcattga tatcgagcag gtcggccata tgcagcttag cagtttttcc
    16501 gatcagccca tcggagcgcc gctcaaggcc cggcgaatgc tgatgaacga cgtctgcccg
    16561 gtcacaaagg tcaaggggca ggagccgccg gccgacgtga gcgtttccac gatcctggag
    16621 gtcccggcgg ttttgaacta catctgggcg ttcagcgaca aaggcattca aatgatcagc
    16681 aaagccttgt caaccggcga gaaccggagc cagttccggg atgatccgga ttcgatctgg
    16741 aaaccgccgt tctcggttgc cttggccatc gagaagtctt tcggtgagaa cgagcctaag
    16801 agccgggtcg tcgtgctcgg aaccggggca tctctgacgg actggcatct aacgcggcgc
    16861 gtgccacggt tcctgggcga gggcaagcgc gcccggttcc agaccgatcc gccaccgacc
    16921 gagaacgcgg atctgtttgt caatgccgtt ctgtggctct gcaatcagga agaaatgatc
    16981 gcatccggcc cgtcgcagat tcccttggtg ccggtgctga aggcgggaac ccagacgaag
    17041 gtattgaccg tgacgatggt ctgggcggcg atcgttttcg tggcagggat cgtggtcatg
    17101 cttgtacgga gcaagtaaga cgccatgaat accaagacaa cacttatact ggcgattctg
    17161 gccgtggctg tggcggcgtg tctgttcttc gttgtcaagc cgtgggagac tcccgtgagc
    17221 gggccggtcg aggagcccaa gaccaccgcc aaggcgatgt ttgaccccaa gccggccgac
    17281 gtggatcgtg ttgaggtgct ggtccgcggg gagcagtctc cccgtgtgtt caaaaaggaa
    17341 accgacggat ggatgatgca tgcgccgact accgccccgg cgacggagtg ggaaatcacc
    17401 aacaacatca tcgataagat cgggaacctg aagtacctca aggagtatcc ggtcggtgac
    17461 aagaatcgcc cggatgacaa tgccacgcga ttcaacaacc ctgtcgcgac cgtcaaactc
    17521 atgaaggaag acaaggtgct ggccgaactg gtcgtcggca acagcttgcc caccggccgg
    17581 ggcaacttcg tgaaaaaggg cggctccgat gtgatctatg agtcgcagga gagcctctcc
    17641 gatgcatttg acaagaagat ctcgaactat cgcaacaagc gggtcgtgaa tgtcaaactg
    17701 gacgatgtga aggagatcac cgcatccggc ttgtcggcct acaagctggt gaagagcggc
    17761 tccgactggc tgatcgagca gcccgagcgg ggccgggcgg acaagagcgc ggtcgaaacc
    17821 aacgtcgcca acccgttgat cagcttgtat gtcgcggatt tcgtagacga cacgcccgcc
    17881 agttacaagg tgtacgggct ggatgagccg aggttgactc tgacgctcaa gagcaccagg
    17941 acggtgccgc ccaaggccaa gccgggtgat cccgacacca agccggccga cacccaacct
    18001 tcgacggagg aagtcgagaa tgtgctcctg gtcggcgggt cgaccggcga aaacaagtac
    18061 tacgcccgcc tcaagtcggc gccgtgggtg ttcacgctga acgccggcac ggtggacaat
    18121 tttgccaaga aggtctccga tctgcgagac aagacgctgg ccaaggttga gagcgcgaag
    18181 gtgatcagag tggtcagcaa aaccccggat ggcgagatga cgctggtcaa gcagcaaggc
    18241 aagtggtcgt ttgccgacgg gacggccagc gatgcgaccg cagtgtctga tctgatcaag
    18301 gccctggcgg atttgaaagc cgtcgacttt gtcgatacct cgaagctgct gatgccgctc
    18361 gactggaaca atccgcgggc cgccgtcacg gtcacccagg aaggcgaggc caatcccgtt
    18421 accgtgctgg tcggaccgga gagttccagc ggcaaaatgg tctacgtcaa gaacgccgcc
    18481 gaagacgccg tggccgccgt ccgcgaggac caggtgacgc agttgctggc cgggccaatt
    18541 gcctacggcg accgtcagat actggagttc ccgcgcgagc gcgcgacgag actcgaaatc
    18601 gcccgtgccg gggccgaaac cgtcgttctc aagcaggaga agaacatctg gtcgatgatc
    18661 agtcccgttc aggcttctgt ggacgcggac gcagctcgga acctgacgca ggatctgtcg
    18721 accctgcgag ccaagggcat cgtcggcaaa gacaagaaag ctttcggatt ggactcgccc
    18781 acagtgacgc tggcagtcta cgtggagccg ctcaccgcgg acccgaaggt caaggtggcc
    18841 tcctcggccc cggcaacgac aaaaccggcg accacgaaac cggcaacgac gcaagcgggc
    18901 aaaaagccga cgctccagga cttgctggat tatacgctga ctcttcccaa ggagaagcag
    18961 aatccgctgg caatcgaaat gctcaaagac atgatcgcca aggagaaagc ggaggctact
    19021 caaccggcat cgcaacccgc tgccaccaag ccggccaagt acgctccggg aatgtctccg
    19081 gaagagttgc tggaattcca gaagagcctg ccggacgacc aacggaatcc gaaggcgacg
    19141 gaaatgctgg agaagctcgt tgccgagcgg aaggcagccg agagccggac ggccgaggag
    19201 cagcctgccg ccagtcagcc tgagccgcct cctccaccgc cgacaatcca tcgcctggtg
    19261 ctcagtcaaa agggcggcaa gacttacgcg tgccgtgagg atcgcgatct cgtttacgag
    19321 ttggaaaaca agatctatga cgatgccacc gcggagatgc acgatcgtca ggtggccagg
    19381 ctggataccg cgacggtaac cgatctgacg ctggtggttg gcggcagtga actcagcttc
    19441 cgtaagtccg gggacgagtg gacctatgtg gccgatccgg tcctgccgat tgacaagacc
    19501 aagattgatg aggttctgaa cgccgttcgc gacctcaaga cgcatcgtta tgtggcatac
    19561 caggcggacg acttggccaa gtacggactg accgggcagg ttgatcgcgt ctcgctggtg
    19621 gccgacggcg gcaggcggat cgagatcctg ctctccccca cggggcccgc gggcgatacc
    19681 gacaagagcc gttacgcggt cctttcgggc agcaaggctg ttttcctact caaggacgat
    19741 caggtgaaca agttcactaa gaagctcgac gacttcgaga aaaaggcctc gtaaaacgcc
    19801 accgtcagag ccacgggggg cctgttgggg cccttgttgc cgggggcctg agtcatggcc
    19861 gattcccagg atcgcgtctg ttgcaccgtg ctttttgcgg gggccgtcca gggggtgggc
    19921 ttcagatatc ggaccgagcg ggtggcgcgc gactacgagg tgaccggcta cgtccgaaac
    19981 cttcccgatg gacgggtgga actggtggcg gagggcctcc gacctgaggt cgagcggttc
    20041 ctcgatgcgg tagaagagtg catggctggc tacatccgca gtcgacagcg gcaggacgcg
    20101 ccggccaccg ggtgccaccg taagttttcc atccggttct agcccggcga gttgtttgcg
    20161 ttttttcggg tttgcggtac aatccgaaga ttgcttttcg acttgttttg aaaaccagga
    20221 tgggtccgtc atgcccaaaa tgaagagaca caagggttta gataagcggg tcaaggtcac
    20281 cgccagaggc aaggtcaagc gccgcaaggc gttcgccgga cacctgctga gcggtcggtc
    20341 tggtcgtcgt ctccgccgcc ttaagaaagc ggcgctcatg ccgaaggccg acgcccgcaa
    20401 ggttctcttc gttctgggca agtgacgaac gtgaggattg ccagaaggtc gccatccggc
    20461 ctggtgttgg tggggcgatc gacgcaggag accgagaatg ccacgtgcaa cttgtggagc
    20521 cgctagggcg aggcgcaaac gaaggctccg cgccgcgacg aagggttatc gcgccgctcg
    20581 cagcaagctg acccggacga tgaagaacgc gctgacccgt gccggccagt acgcgtttcg
    20641 cgatcggcga gccaggaagc gggagttccg ctcattgtgg gtgacccggc tgaccgccgc
    20701 gtgccgcgcc cgggggatca gctactcgcg attcatgagc ggcctgaaga aggccaatgt
    20761 catcctgaac cgcaagatgc tcagcgagct ggcgattcat gaccaggccg cgtttgacaa
    20821 gatcgtcgcg ctggccaagg cataaacgcc gttcccgtgc cgggcggacg tttcttcacg
    20881 atcatttgcg ggccgatcgg cgctacctgc gtcggtcggc cagcttctta taaagcgatt
    20941 cgatgtcatc gaccatgcgt tcgtggtcga aacggcggag gcaccattca cgaccggcgc
    21001 ggcccattcg gtcacgcatg gcggcatcgc cggcgagtct taccatcgct tcggcaagcc
    21061 cctgaatatc gttcaagggc acaagcaggc ctgtctgatc cggcaaaacc acctcgggcg
    21121 ctccgtcgat atcgaagctg atcaccggtt tggacatgat caggccctgc acgactactc
    21181 tcggcagccc ttcccactgg gaggcgtgga ccacggcatc cataccggcc agcaggcggg
    21241 ccacctcaag cggcggcaca aggcctgtca ggcggactcg gttggtcagg ccaaggcgca
    21301 gcagttgcga ttcatattcg cttcgctgag atccgtcgcc gacccacaca aagcgcaggc
    21361 tggggcagcg gcgggaagcc tccgccattg cgggaatgag ctgttcatat cccttgttgc
    21421 gaaacaaccg agcgacggtg ccgaagacca cgcagtcctc gccgaaaccc cattgttgcc
    21481 tgattgcctg acggtcatgt cttgcggggt cataccactc ggttctcatg ccggagtaaa
    21541 tggtcacaaa ccggtcgggc gaagcaatgc cggcctcgac ggactggcgt atcatggcct
    21601 cggccacgcc gatcagggcg tcggtatacc gagcacatcg acgctccagg aaacgataca
    21661 gggagcgaat cggagcaggc tgagtgcgat tgaagctcat cccgtgaatg gtgtggacga
    21721 tgatgggtac tccgacccgg tgggcggccc agcggcccag gatgccggcc ttgctgctat
    21781 gtgtgtggac gacgtccggg cacagcttgt caaaaagcgt ccacagttgc cgacaagcgg
    21841 ccaggtcggc aaaggggcga accgcacggt gcatcgccgg tatgatccga acgtcatagc
    21901 cgcgcgcccg agtctcttcg aagagcgagc cctccgggcc ggtgtcagga ccgacggcca
    21961 atgtgacctt gtggcctcgt cgcgtcaatc cttcacaggt cagcagcgtg ttttcctgtg
    22021 ctccgccgat gatcagtcgg gtgatgacat ggcagacgtt catgagttct ccgcggcagc
    22081 aggctcgtcc ggaacggaag tgatgccgag ccctttccag ccgggcggtc gcgtaagatc
    22141 gtatttgacc cactgaaggc acaaccccct ggccggtgcg gtcggccccg ccaatcgtct
    22201 gtcgcgggcg gccaggatct cgtcgattcg ttggggggcc cagcgtccac gaccgatttc
    22261 cagcagcgtg cccaccatgt tccgcacctg attgtacaga aagcccgtcc cttcgacgtc
    22321 gatcaggatc tcatcgtatc ggcgggaaac actcgcctgc aagatggttc ggacggtcgt
    22381 ttcccgctcg ctgcctttgc tggcgaagcc ggcgaaatca tgtgtgccga cgagacggtt
    22441 cgccgcttcc tgcatgcgca tcgtgtcaag ttcgttccag aagtggtaag tgtatcgctg
    22501 cagatgctcc tgcaccggtc ggcgcgtcgc attgtaaatg cggtagcgat aaagcttcga
    22561 taccgcgtcc tgcgagcacc gaaaacccag gggaacctcc gagacgtgca gcagagagac
    22621 gtctttcggc agacgtgaac cgatggccct tcggaggttg atcagcggaa tctcacacgt
    22681 ggtttcgaaa ttcgcctgct gacccgccgc atggacgccc gcgtcggttc ggcctgcggc
    22741 gttgagcccc acctggtggc ggaccacgcg gcgcgtggcc tgctcaacaa catcctggac
    22801 ggttcgcatc tccggctgct tctgccatcc gtgaaaatcc gtgccgtcgt aggcgatcag
    22861 gaatcggatg ttgcgctcca tggccggatg gtcaggcgac gagttcttcc tccagtttgc
    22921 ctttgaagcg gttgcggagc cgcgtaatca cgtccttgtg gatctggctg acgcgggatt
    22981 ccgacagatc gaggaccacc ccgatttcgg ccatggtaag atcctcgtag tagtatagga
    23041 tcagaaccag gcgctcctcg cggctgaggc cccgcgtcag atagttggtc agcatctggc
    23101 gggccattcg ggccgcagga tcggtttgcc gccgatcgcc gatatcccag ttccgttcgc
    23161 tcgaacggct gcgcgaactc gagtccttcg ggtccatcgc ggagaaatgg acctcgcgcc
    23221 cgagctgcga gattcgtgag agctgatcat agcggtcctg cgatagcccc atgcgcttgg
    23281 cgatctcgtg gtgcatcgga ggacggttca gctccgcatc aagtagttca cgcaccatca
    23341 tgcgctgttt ctcaaaggtc cggacggtgc gcgactgggg atcgagactg cggagccagt
    23401 ccatcacggc gccaacgatg cgctgctggc agaacgtctc gaacttcgct ttcctggtcg
    23461 ggttataggc ctcgactgcc tcgatcagcc catcgtagcc ggcgctgcag atctcgtcgt
    23521 agccgatctg cgccggcaat ttgcgggaca gccttgcggc ctggatgtga accaacggag
    23581 cgtagtactc cacgaccttg ttgcgggcct cgtcgctgcg ggtcttgaag taccggttcc
    23641 acagagcacg gatatcgtcc ttcttgtcag ccatcgcgaa cctcccactt gaagctccgg
    23701 cacgcacaaa tcaccgcacg gacgaacctc ccggcggggg cgcggaacgc gtgagggtcg
    23761 cttctttcga gccgcggaaa atccaaccgt cgagcgaaaa cggcggaagc cgcaacccgg
    23821 tgtacacgtt aacctgttca gttcactcgc tggaccggct agaagtgaaa gcttccttac
    23881 cggttccttg gggaaccaat ccctggatcc ccgcgatact ccctggtgac atcggaccgt
    23941 tgggattgtg gtcgctcgca tggatgggtg ttcgccgagc gagagcccaa ccccctcgat
    24001 gccggtcgat cgctgtcacc gagaatctat ataactcaaa aacacgccca tgaaaatagg
    24061 gtaaaccggc aatatgtcaa atttttttgg ggggcaatga tggcagatcc ggagagatga
    24121 gatacttggt gtagcaaacg tggcggaccg acgtcatttg cgtcaggagc gaatgcgcac
    24181 acacatgcgc atcagcgcgc caggacccaa gctaacaaag tcctaattca gtgtttgcgc
    24241 gcgggcgttt cgaacttgac agcgccgcgc cgagaggcgg ctgctgattc gtcaactcgt
    24301 ggatcgtgac aacgtcgctg ttcttggggg tgatcccagt ggcgattctg gccgcgacct
    24361 tgtcttttcc ggggattcgg ttttctcctc gcgcccggat cggctaccgc gcaatggatt
    24421 gcttcgatgc cgcatgagtt taagtcatcc tcggaactgc ctgtgagttt ctttgagcaa
    24481 ccacgaaact cacaaccttg acggcctctt gaaccggcct cagagcagct tgatcagccg
    24541 ctgattgacg ggctgaggct gacgagctga ggttgccgat tcggatcagg cccggcgctg
    24601 atggtggtgt gcgcggcgcc aacaagtcat cggagtctgt ttttgcctgt tttgtcagcc
    24661 gatctccctg cgactgcaaa cggcctggat cggccggtcg tacagcgcac gacgaccgcg
    24721 gaatacgacg cgacctgacg ccaggtcgtc acggcgcgag tcggttttga gattctggtg
    24781 tccttcgata tgaccgaatc agtacctgtc gatgactgtg gagctattcc ggattgtctt
    24841 gcccgatcgt cacccttttc attttgtccg aatccagcag atgctggcaa ttgattactt
    24901 tctcgattag atcgaggggg agataatagt aatggttcga agcttcgtat ccgatgcggg
    24961 catcggcgcg ggtcaggtcg tacatgcgcg cggccagcct catttcctct tcgatgactg
    25021 agcgttgtgt ggtggtgagt tgtgccacct cggcgatctg tggcgtgctt gtgtgtatcg
    25081 gcggtcgagg cttgctcaac gcgtcgcgat tgagaatgaa tcgtgcctgg ttggcgaccg
    25141 acatgaagtg tatccgtgcg gcctccgcca gacgaagctc ccctgcgatg gtccggctga
    25201 atgtgtccgt gtcagctttt gtctgggcct ggcgcagcaa ctcaatgccg gtcgcaaacc
    25261 ccctcgcgac tttctcgcac tgactcacgt acacatccgc cgggtaagag cttcgccagg
    25321 catccagacc gtcgtagggg aagccgacca tcgttggacg gtatcccgtg ggtgcaggat
    25381 acagcgggtt ggcgggccca agctgaaacg ggcctgcata cagccccggc attccgtaag
    25441 ggtactcctc gaaggccgtg ctgaaggccg cccatgcctt ccgcacttct ggagctgcgg
    25501 ttttgccgta gagatcctcg gccacgcggg ccaatgcctg ttcagccgtc ggcggcggcg
    25561 tcttgctgaa ctcgcggacc agttgcagat tgggcgaagg gtatccgccg agactccaac
    25621 tcaacatcat gtcacggatg cccaagtcgc gaaggttttc gatgtgccgg gcgacgaggt
    25681 ccattgccgg caggtatggg atggccgaca gctcccaggt gcagttgacc tgaaccttcg
    25741 ccatcgtctt aagcccgcgc tgttgggccc acttccagtg cagttgggca cgcggccccg
    25801 gaccgaccga agatatcgag tattcaccga cgatcgtttt tacgccgcct cgctcgatcg
    25861 gtagatccca ctcgctgacg ctcatgaggt acacgtcgtc gggtaaacga cggatgattg
    25921 cctcggccca ctcgttcttc cagccccaat cccagcagat gatcgtcgcg ttgggattgc
    25981 ctttgcggac tccttcggcg attgcggcat tgacatcggc gatgatctcc gccggcgagc
    26041 gtttcgagca tcgcggacac gaagcctgct ggtagtggct ggcgcaatgg gtcaggtttt
    26101 ccgacgcggt gatggtgaaa accccgccca gatcgggcac ttcacggaag acgtgcgcca
    26161 gcgagtcaac caggaaccgc cgcacgcgat cggtgctggt gcacatcgcg gtgttgtcgc
    26221 cctcgcggac gccggcgatg tccgcacgat cctcgaagaa cgtcgacggc atccatcgcg
    26281 gttcgttcat gtagagatac accttcacgc cgtgcgcgcc ggcccgctgc acgagctgcc
    26341 gcaggttggc cagccttgtc tcgtgccctt cgccgaactc gggaaagtct tgcgacggcg
    26401 caaggtcgcg caggacgacg tggagccaga cgccgttgac gcccaaacca accagccgtt
    26461 gcagcagacc gtcgggatag ggattgagcc gggcgtcgag cagggggtcg ccgaagaccg
    26521 cgaaataaga ataaatgaaa cggatcggtt cgtcaccggc ctgtggacca gggggcatct
    26581 ccgacaaggg cttcgacagc tcctccacaa aggcgaaccg tggttgggcc ggctgcccga
    26641 acgactcgcc gaactcacga gtcaccagtt ccttgatctg ggcgcatctc ttctgtgtcg
    26701 cctcggtggg tggctcgtat cgcagcggcg gacaatcggg tttcagggcg ccgagcttgt
    26761 gccagagaaa atcatcctct ttgagagtgt acgcgagttt ttcggccttc cagccgagca
    26821 ggaggagcaa ctgatcgtag ttcagcaggt gccagttgcg gcggatgagg ccgatgtagc
    26881 cccgccgttt ctgtatgtcg gtaatcggaa cgtgttgtgg caaacccatg gattcgccga
    26941 tttcgcggac gctgtcttcg gtcgtgccca gcacgcgagc catctccgac aagtccgccg
    27001 attcccagtt ccgccagacg aaggcgtgca gccgatcggg gaagtgcgag gcctcgattg
    27061 gtttgggcga agtgccggca tggagcaccg gctctccctg ggcggctcgc cctgttggca
    27121 caccgccgga caggatagcg gccagggcaa tctgacatat cagccgcctc ggtgatgcgt
    27181 cagccggcgt ctggcgggtg acgcgcgctt gggtcgttcg attcgcgact tgtgaacggt
    27241 tcatggctgt agacctcctc ttggccagat cttcttcggg ccaaccggga aaaaagtcaa
    27301 gccgggtcgg atgggcaggt gctacagcac ggccagaatg atgttgacga taagcgaaac
    27361 gcctagcagg gcggcgagca ccaccaccca cacgaccggc acgaccagcg gcttgggctc
    27421 gcttgattgc tggacggccg gtttttcctc gaccggctcg gcactctccg ccaggccttc
    27481 gagcaggtga gcgtcgtatt tcttgcgggc ctgaagcggt ggctcgcccc gggcgatggc
    27541 ttccagatcg gtcagcaggt ctcttatcga ggcgtaccga tcctccggtt tcttggccat
    27601 catccgttca atcacctccc cgacacccgt cgacagggag ggaacgatgt gatccggcgg
    27661 aaccaatggt tccttgagat gcttgtgcat gaccgccgcg ggcgtcgacc cctcgaaagg
    27721 cagcttccct gtcaccatat ggtagaacgt ggcgccgaga gagtagatgt cggcccggaa
    27781 gtcgatgttc acctcgccac ggatttgctc ggggctgatg tagtacgggg tgccgtatgc
    27841 acggccggcc tcggccatcg ccgctttcag atcgcccgtc tcacgcgcca ggcccatgtc
    27901 tgccagcttg gccacaccgt gacgcgtcag catgatgttc ttcggcttga cgtcacgatg
    27961 gacaaagccg cgttcggcgg cgtgttcgag agccttggca atctgaatga tgatgtcgag
    28021 agcttcagat tccttgtaga tcttgcctgc ggccagatcg tcgtaaacgg tcttgccgtc
    28081 gatgtactcc atgacgaagt agtggtagcc gctggcctcg ccgacgtcga tggcctgaac
    28141 gatgttgttg tggttaagac gcgccgccgc gcgtccctcg cgatagaacc gctcgacgaa
    28201 ctccgagttt tcgccgagct tcttaggcag aaccttgatc gccacgatgc ggtccaggct
    28261 gagttgccgg gctttgaaga ccgtggccat cgctccttgg cccagtttgc cgagaatctg
    28321 gaaccccgga atctgctgcg cctttcgggc gatcgagtcc tccatttccg cgcccagccg
    28381 ctggagctgc gaacgggtca cgaaaccgca tcgcagcatc gcctcggata acgagactcg
    28441 ctttccttcc ttcgcaaggt tcttctgctc ggccagacag tcatgcagct ctttcaaggt
    28501 gagcagttta ctatcaacgg ccaaccggcc cagttgggaa tcatcgatgg aagggtcagc
    28561 cataatgcac tccggctttc gggtcgtctc gccgttcaca acgcttcggc gagaacctcg
    28621 gtttcaaggc cgcgtaatcc acccctgcaa agaccctccc tcaacctgtt gtccggtcga
    28681 tgcggagagg accttctggc ccgctgctga ttatacccgc ctttccggcg tgcttcagcg
    28741 gggacttgcc ggtccaagca tgtctctcag ccatcttgag gcggccggcg ggtcggcccg
    28801 gccggattcc agtgccgata atgccccacg acagcaagcc acctgatgcg gactgaccgg
    28861 ggcaatcagc ttttgtcgcc agcccgtcag ccccgtcgaa tcgacaagcc ttgcttcaag
    28921 gtcctcaagt cccgccgatg ccagggcgct gacttgcagg aagcccgcct cggaaaactg
    28981 gacgttttgc caggatggat gtgccggcag gtcggacttg ttggcgacga gtacgatcgg
    29041 cggaacgcgg atcataccgg attggtcccc cgtcatcttc ttggggggaa ggcctgtctc
    29101 caaaggggtg gtcaggtcag agacgctcag cagaatgtca gcagttgaag cctgctgata
    29161 tgtccggcgg atggcttcct gctcgatcgg gtctgccgtg tcgcgtatgc cggccgtgtc
    29221 gacaaaaacg agcggaatgc cgtcgataac ccccgggtgc tcgacccagt cccgggtggt
    29281 acccggctgg tcgctgacaa cggcttgctc gcggctggcg aggcgattga tcagcgtcga
    29341 tttgccggtg ttgggtggcc cgatgaccac cactcggaca ccgctgagca ggtatctggc
    29401 ctgatcgacc gactggcaaa gcgccgccaa ctcccgccgt gccaaggttg cctcgccgac
    29461 ctcgatgaga tccaataccg cctcgatgcg cttgggcagc ctgtgaacaa gctcggccag
    29521 ccagatggcc actgcccgcg tcttggctcg aagcagcagc ggcagaatct ctcgctcgac
    29581 gacggtggcc gccgggcagg aatcatccag cagtgccgta ggctcgacga tccggactcc
    29641 ggcctgctgg agcatcaaca atgctcgctg gacgattcgt ggcccgccgt gaagactgat
    29701 atcgaccacg aatgggccgt ccggggcccg tcgaaccgtc acgagggcgt cgtcgatcac
    29761 ttgatcccca tccgcccagc ggcaaaggcg gacctcgttc gtgtccacgg cgttcgggtc
    29821 gatcggacgc cgggagcgaa ggtacttggc gaccacgcgc aaggcatgac gccccaccac
    29881 ctcgatgacc gcgacacccc ccattgccgg gggagtcagg caggcggctt tcagtgcgtc
    29941 ggtttcggac atgtcagtgg ccgggtcatg aagcatcata tggcatcgta caacacagtc
    30001 gccttgcccg ccatgaggag catgggcccg acaggctcgg tgggcgacgt ctcatctcgc
    30061 catccgcggc ttcgcggtca cagtctcatg gaaagaaaaa cggagtgcgg ccctggtatc
    30121 ttcacttccc gaaaaccgat tcacggggag agaacatcag cacgaccgga ggaccttgtc
    30181 acgttgcccg ccgcgttttc gaacgaactg ttcggggggt cgggtacgcc catggcccgc
    30241 aggcccgcag ggttcttggc gtagcggtgg gcgatttcgg gagaaatgcg ccggttgcac
    30301 acgagactgg ccaggttttc atcaagggtc tgcatacccc acttcttgcc ggtctggatg
    30361 atcgtttcga gcgattcgat tttgttgaga cgaatggccg ctcgtgcggg atcgctggcg
    30421 gtcaggacct ccatggccag aactcgtttc tgaccggctg tgacgctggg cagcaggtac
    30481 tggctgatca ccaggcgcag cgatagagag agctggcagc ggatgtcctg ctgccgctca
    30541 agaggaaaaa tgtcgatcag tcgcgtgatc gcgcccttgg catcctgggt atgcacggtg
    30601 gacatgacca gatgaccggt ttcagcagcg ctgatcgcca tgcgcgcggt ttcgacgtcg
    30661 cggatttcgc cgcacaggat cacgtcggga tcctggcgaa gcgcggaacg caatccgtcg
    30721 gcgaagctgg ccacgtcgat gccgacttct cgctgcgtaa tcacggtatt ggccttgggc
    30781 agaaacacgt attcgatcgg ctcttcgatg gtgatgatac ggcgatgacc gagccgattc
    30841 atctcctcaa ccaggccggc cagcgtggtg gtcttgcccg accccgtgat gccggtaatc
    30901 agtaccaagc cgctgtgcag attgacgatt cgttccacca ggtcttgcgg gattcccatc
    30961 cactcgaagg tgggaatcga ggacggcacg aaacgcaggc aagctcccat cgagccttgg
    31021 ccgaagaaga cgttgactcg gaaacgaacc acgccggccg gtcggcccac gctcagcgag
    31081 aagtcgaagt ctttctgacc gtcgaggcgg gaccgcaact cggccggcat gatgctggag
    31141 atgatgcggg ccgcagcctc tgtggacaga gggatcttgt cagccgggaa caggtcgcca
    31201 tggatgcgca atgtgggcgg atggcccgta accaggtgca ggtcggaagc ctcacgggac
    31261 aggccgttgt cgatcaattc cagtagctgc ggatcaggtg atggggaacg ttgtctcatt
    31321 cgatgcgttg cctcgtgcaa aaccggtatc ctgcccagcg gagatggctt gcacttatgg
    31381 gaccggcgtc ccggtgcttc aataacggac tcctgctcga ctccggaacg tcggctgacg
    31441 ccgggctgat gctcctgcgg gtcggcttat aacctgatat gcatcggccc ggcagtagtt
    31501 gcatcaccag gttctcagcg acaaacacct ggctggtctt catcggtgcc cgcgtgttgc
    31561 ttgtacgcga tcaccaaacc gtccaggcac aggtcaggca tgaccttgtc cacgaaatcg
    31621 gccccgcggg cgatgtcggg ggcgttgcct cccgtgataa ccaagggcgg ccatttgccg
    31681 atgtcggtgg catagcgttc ggcaatttcc cgcaatgccc cgaccatcat cgcgtagatg
    31741 ccgttgcgaa tcgcgctttg cgtgtccttg ccgagggtct cggtaggaca gccgacctcg
    31801 accagcggca actgcgcggt gtgttcgtgc agagccctgg ccgacagggc gacacccggc
    31861 aggattgtcc cccccaggaa tatgccgttg tccgccacca aatcaatagt cagcgcagtt
    31921 ccgaagtcgg caaccacgca tgcccccctg acacgggcaa acgccccggc ggccgcacag
    31981 agccggtcgg tgcctactct ggagggctcc cgcagcgcca agtcaagcgg aatcggcgat
    32041 tcaatctctt cccccacaac cagcagcggc ttgatgccct tggccgcgca tgcctgtctc
    32101 aacttggcga gcaccggcgg gcagactgaa cagatcacca cggcgcgcgt cgccgtcccc
    32161 ggaagctcgt tccacagacg ttccaggcgg ttcatcagtt ggtcaaaaga gaccgtcgga
    32221 atgtgttcgg cttcgtcacg atggtcatcg aaccaactgg ccatggccac gttcgtgttg
    32281 ccgatgtcca tcaggatcag atgagccttg ccgcttcgtc cggattccgc gatcacgatg
    32341 cgcctcctgt cagatcgtcg gccgtctcat tatcctcgaa atctccttgc gccgcttcag
    32401 aggactcttc gttcgttgaa agcaattcct caaagtcggc ctcgtcaggc tcttctccgt
    32461 ccggcagaat accgcggaac gggtcgtcct tgctcttgac cgcaagagcg cttgccgcaa
    32521 cccgctcccg ggctttggcc gcctcgacca tctcccacaa acgatacccg agtcgttcaa
    32581 ggccgcttcc agtgacgccg ctgatggcta tgacctcctc ggcggcaatg cccaaggctt
    32641 ccagctcgtc gcggagttga tccaccgcat cattgttctc ggccaggtcg atcttgttgg
    32701 cgacgatcaa ctcgggtttg gccgcgaggg cctccgagta ttttcgcagt tcgttgcgaa
    32761 tgatccggta ggcttcgatc ggcctcgctt ggcccggaag ggggtggacg tcaatgaggt
    32821 gcaggatcat ccgggtgcgc tcaatgtgcc gcaggaagtc gtcgcctagc ccgtgccctt
    32881 cgtgcgctcc ctcgatgagg cccgggatgt cggccatgac gaaacgctga tatccgggca
    32941 tttcgacaat cccaagctgc ggctcgaggg tggtgaacgg gtagtcggca atcttgggac
    33001 gagccttggt cagccgagac aatagggtgc tcttccccgc attgggcaac ccgacgaggc
    33061 ccacgtcagc gatcagcttg agctccagac gcagccgacg ttcttggcct tcctcgcctg
    33121 gttggcagta tcggggggcc tggtgacgag ccgtggcgaa gtgggcattg cccctgccgc
    33181 cccggccacc ccggacgatt cgcactttct ttcctggggc gtccaggtct ttgagcaggc
    33241 gtccattgtc acggtcgtaa accagcgttc cgggcggcag ccggatgaca agatccttgc
    33301 cgctgcgacc ggtcatgttc ttgcctttgc cgggtttgcc gttctccgcg acccagtggt
    33361 gatggccggc catgtccaac agcgtggcca cgctctcgtc ggcgacgagg tacacgctgc
    33421 cgccccggcc tccatcaccg ccgtccggac caccctttgg aatgaaccgt tcgcgacgga
    33481 agctcatgca gccgtggccg ccgttgccgc ctcggacata gatctccgcc cgatcaatga
    33541 acatcgtcaa accttccccc cttaatcagg gggtgaagaa accctctccg cccgttcata
    33601 ggggcgggag aaaccatcta aaacccctta ataaggttca ttgaagccct atgccccctt
    33661 agtaagggag aatgaacccc tctccccctc cccttaatca ggaagccgcc cccctctccc
    33721 cccttaataa gggaaccaca cccctctccc ccccttaata aggggggcag ggggggtagt
    33781 acacgcgaaa cgactcctcc catcccacga ttcgatcccc cttcatgccc catggcaaac
    33841 gcgagtagag catcgcagag cctggggcgg cagctcttcg ccgcccccga ctttgcctgt
    33901 cgctctcaag cgcactctcg ctcaaaaaca aacggccgac acgacgtcgg ccgctgggaa
    33961 ccctttcggt tgaactccgt gcatgccgcg gcacccgatc ccttgtccgg cgcgtgaccc
    34021 gacccggcgc cccgacggac aggcggatgg ccgaggctcg gatggtcagt ttggcgcgat
    34081 gtccacacgt cgacccttga agaccaccgt gccgtcgacg agcgcaaaca aagtgtcatc
    34141 cttgccgcga cctacgaaac gacccggttt gaagaccgtg cctcgctgcc gtacgatgat
    34201 cgagccaacc ttcgcttgct gaccaccgta gagcttcacg cccaggtact gcgggttgct
    34261 gtcgcgaccg ttgcgagtcg aaccttgtcc ctttttatgt gccattgcgc cttgctccca
    34321 taccgattca gtcgttccgt tcgttgctta tcgcatgatc cactgctgct tatccgccaa
    34381 ccgctccggg atgacgctcc ttcgcgtaac cgggctcccc ggaaacttgt acggaatcgc
    34441 caatgtcttg cggaacgtga aataccgctt attcgtctca ctttccggtt tgctcgcgtc
    34501 aaaggccgga ttcttctcgc gcctgacctc gcccgacaag ccggcaacga aaatcgtaaa
    34561 gcctgtggcc ttggggtcga agtccctgaa gatcgccacg ctgtgccggg ctcgatcctt
    34621 gcctctcagc agtctgccga tcgcccgctc aggcgccacc agcaacgggt cgttcgcccg
    34681 ccgctcaatg gcccggaatg cctccggact cacccgaacc tcgctctcga tcacctgcag
    34741 ggtgttggtg accagctcga acgccgggta gaagtccacc tcctggtcgc tcgggttatc
    34801 aaccctgtaa agcatgaacc agtacaccac gggctcgtcc ttgcccggga caaccaccga
    34861 tacccgctgc ggatcctcga acctgaagtt caactcccac gcaaccggtg ccgccgacgg
    34921 ctgaatagcc accggggcgg gttgggacgt tgccgcggac agcatgcata agcttatgtg
    34981 ccgcaagacg ttaagcatgt tggagtcccc gtgtgccgtc tcgtcgccat atccgtctat
    35041 cgaccgttcc gcgcgcattg ctcccgctga gcaaacaact gcattctacc caccgagtcc
    35101 gccgcgtcaa gccgtgcttg tcccacgacc gcccaagccc tccgggacat tcctggcggg
    35161 gagaggccac ggtttgttca agacgacgac cgcgaggacc gcccccgtgt gatgttggcc
    35221 gctctgcggt ttgccccccc gcttaaactg gaggttctag aagagtcgcc cacgctgaca
    35281 tggaccctgt ctcctcgacc attagcctcc acctgggatc cctgctcacc gccaccacgg
    35341 gctcgatgaa atagccactg gagtcgtcgc atccgccgcc gcatagaacc ttacggccgg
    35401 atatgtgtgc cgcttgcacg catgtttctg gatcccggta cgcttgggtt gagacccgca
    35461 aaaaggcggt ccgccttagg ttctaagcca attcccaccc tttccgtcga ggcggatcca
    35521 caaatgtgtc agcctcccga gtgttggtga tcctttgccg ctcggcgtcc catcggatct
    35581 tcttgccgct gcgatatgcc acattgccca aaagcaccgt ttgagccaga gccccgctgt
    35641 agtcaaaatt gcacgacgtt ttctcgcccg acttgcaggc ctccagccat tcgagatgat
    35701 ggccgatcga ctcgggtatg cagggttccg gccgcttgta gtcagcaaag tctttttccg
    35761 gcaggagcac gtgccgatca tagtcggcca gcaacattcc cttgtcgccg acgaacagga
    35821 caccatctcc ccattccgga agcttgcctt cctggaaata gtagggacgc cgtttgccgt
    35881 tgtaccaggt cagaaggacc ggcggcatct tgccgcgagc gggatagtga aacctcacaa
    35941 tcagccacac gggacagctt tcggggtgga caggtggccc ctcggcctca atggtcgttg
    36001 gatgtcgcag atccagcgcc cagtgaggca ggtccatgtg gtggcaggcc atgtctgcca
    36061 gtgtgccgcc accgaagtcc caccagccgc gccagttcgc aggcagatag gctggatgat
    36121 aaggccgata cggtgccgga cccagccaca ggtcatagtg aagcccttcg ggcactggcg
    36181 gggtctccgt cggacgctct tggccacccc actcgcgttc gacccagacg tgaacctcgt
    36241 gcaccgcgcc gatgccgccg gattggatca cttcgacgac acgccggtag ttgtttcgcg
    36301 cgtggatctg tgtgcccatt tgtgtggcca cctttgcccg ggccgcggct tcgcgcatga
    36361 gctgcacttc acgcacagtg tgtgccaggg gcttctcgca atacacgtgc ttgccaagcc
    36421 gcaaagccgg cagcgcagcg gcggcgtgcg tgtgatcggg cgtcgcgatc acgaccgcat
    36481 caatgtcctt ctgatcgaac atcttgcgga agtcggtgta gaaacgggcc ttgggaaacc
    36541 tctgtcgtac ttcggcggcc ttgttctcat cgacgtcgca gagggcgacg atgttgcaga
    36601 tctcggcagc ctcggggccg gtcacctgac ggaggttcca gtgcccttgt ccggacacac
    36661 cgatcacggc cacgccaagc ttctcgctcg gcgacgtcgt ctgaccgagt gtctgcctcc
    36721 cgctgaccca gaaacccgcc ccggccagtg ccgaagcctt cagaaactca cgacgcgtct
    36781 gtctcttact cacggatgct cctcttcaag tcccagtctc attgggcaat cggattataa
    36841 ccaatgtgga gaaaacggac aatcccgcca cttcttcaca gcggttgaga gaaacggctt
    36901 cgacaccgtc cgccacacca tggcttaagc ccgcgttgcg aaacgtatag ccggactcga
    36961 aatcacacgg aaggtccgct ccgggtcttt cgcacggcca gaaaggagat gccgccgagt
    37021 ttcagcaaga aacgcttgac cgggctccgc gaaaaggtag actcctgttt ggttcaaaag
    37081 tgaataacag gcccgcaggg tggcaagcgg tccgaagaat gtacgatgaa tggcaatggg
    37141 ttccaggaac gagcctgtgc cgttcgctga cttgtctcgt tttcatgaaa tggaggtatc
    37201 ggatggcttg tttcacttct gtgggcggaa aaggtcgtgc aggccgatgg tttcaagggg
    37261 ccgcaattcc gctgctctgc ggcgtcgcaa tggtaatggc gctcagcgcg gccggctgcg
    37321 tggtcgatag ctctcacccg cccgagacgc tctcgggtga gcagaccatc gaggaggcgg
    37381 agacctccga cggcacggcc agcaagtccg tggagtcacg atggacggcg ccggaccaga
    37441 atttcgttct cagcgttgag gtgaccagcc ccgagccgct gggcgtcgaa aacgcccaac
    37501 tgcaagtgac tctgacctgt cagctcggca cgacgaaaac cttccccgtc actctcacgc
    37561 ccgacgggtc gagcaaactc gttgccagtt tctcacgcgc cgccgatgcc cccgacggca
    37621 gcgagtgcgt cggaatcggt gggcagaaag aggccgccaa gtggaaggtc accctcagtc
    37681 gtccggcgga cacgacgcag gcgctgactt tcaagtacag gatggactac cggctggcct
    37741 caatcgagac gccgtaaggc atgctttcgt gaatcacatt tcggttttcg gcttgcacgg
    37801 caggtgagca tggagggcag gccacagccc agcggcgacc tcgctggccc cagaatccgt
    37861 tctggggcct tccgggtgcc tcactggccc cagaatccgt tctggggcct tccgggtgga
    37921 ggattctcat cctcactgcc ttcaccgaat gcggaacgaa taccaccagg cgcagccgcc
    37981 cggcacgggt gcgtagggca ggtcacagcg aagcagcgac ctgccaagca tgtagggcag
    38041 gtcagagcga agcggcgacc tgcctttccc gcccaagatg ttgcggcagg tctcgcttcg
    38101 ctcgaccgtg ccctaccacc ctgtaccttc gtgattcaca tctcggtttc gagcccgagt
    38161 gcggacttgt cggcccaatc gatctgctcg ggtgtgtccg gcacggggca atgacgggct
    38221 tggccgatgg cccgaaacgc gtcggttgtc gtcacgccaa gcttggccag aatgcatgcg
    38281 gccaccagag ccgagcggcc gatgcccgcg cggcagtgga tggcaaccgc cttcccgccc
    38341 ctcagatcca cggccagccg ctcgatgaga tctcgtgtcg cccgcgggtc cgcaggtacc
    38401 gaccgatcct cgatgggcag ggaaaggaat tcgatgccgg ccgagtggca gcattcggtt
    38461 tcctgctcca acccaagctc cgcgatctca tccggtgtca gcagcgagac gatgacgtcg
    38521 acaccctggc gacgaagtga tgcgatctcg tcgcccagcc actcaccacc gcgcgggcgc
    38581 ggcatgatcg ccaggcgata ggggaggggt ttgatccagt agatctcggc gagcattggc
    38641 agctctcaca caagaagcat gtagcgcagg ccacagcgaa gcggcgacct cgctggcccc
    38701 agaatccgtt ctggggcctt ccgggtggag gattctcatc ctcattctcg ctggccccag
    38761 aatccgttct ggggccttcc gggtggagga ttctcatcct cattctcgct ggccccagaa
    38821 tccgttctgg ggccttccgg
//
LOCUS       DAOTPW010000014        37768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1184_length_37768_cov_5.833294, whole genome shotgun sequence.
ACCESSION   DAOTPW010000014 DAOTPW010000000
VERSION     DAOTPW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 37768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37768
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1184_length_37768_cov_5.833294"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="P5316_02200"
     CDS             complement(<1..1009)
                     /locus_tag="P5316_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRR83798.1"
                     /translation="MAGQPKGPFYAVLGIIIVGLVAFAIYRSDLFAPREKTPGTDVAL
                     LPGGEQVEDASNPTGVTTVKEYTFKPVERLPQVKGTSAYKPMTDNTVRFALNVWAGWA
                     PIIYANNGFRPGKVWKTPDGKDFKVELVLIDNPVAMRDAYAAGDVHIGWATLDMVPLF
                     MEGFVDVTGKPRDSRVMPRIYQQVDWSNGGDGIVVRDYIKTVSDLRGKKLVLAQNSPS
                     QYFALNMLVSGGVQPSEVNMIYTEDAFQAAAAFNAQKDIAGAVSWAPDIYNLEKVKGN
                     RMLVTTATANKLIADVWFARADFAQDNPGIMESLVRGIFDAMVDLKEETNRKKVAELM
                     AA"
     gene            complement(1144..1953)
                     /locus_tag="P5316_02205"
     CDS             complement(1144..1953)
                     /locus_tag="P5316_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRR83799.1"
                     /translation="MKTVTGNNPVTKRLACGGWQAGLFVLSLELLLYGCDRSPPPTSP
                     HRSPAPMTAEVATHLIPLPQADQRLCTAVAILVDTSGSMKEEVVDHQGVRRPKYLIAR
                     DALKEIVDYTGQWEKTHTDRVLEMALFNFSSSPSSVLRMGRFDLAGTQQALQTIPQPG
                     GGTAIGEAIEEGFKALYQSGCVRKYLICITDGENTAGRPPDRVARQLHAQTQGEVEMH
                     FIAFDTSATKFGFLSEVNGHTTEAADGAQLRARLTDIYEKRILAEAPAEKE"
     gene            complement(2066..2473)
                     /locus_tag="P5316_02210"
     CDS             complement(2066..2473)
                     /locus_tag="P5316_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83800.1"
                     /translation="MAKKKAMVKRKKARPAKKGAAKSSPKKRKPRTPTAGAARSRSSS
                     WLDANSHKPLIDQYARKLTSFMEAVADGRIEESELAAQEKRLVRLMKEIEPQLSDALH
                     EKITRLLCELTAYDLMQMLHSMQEHRPQATFQG"
     gene            complement(2491..4302)
                     /locus_tag="P5316_02215"
     CDS             complement(2491..4302)
                     /locus_tag="P5316_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR83801.1"
                     /translation="MTQTGSPTPDPSPIMHDSSLITHRSSLPAWYPDWAKQLADLYFS
                     GTTCLFVLHGNVHDLVRCPTRDGEGYVNLSQFLATQVFGTWDIVLQYDLGRGLRPLAG
                     SDPQRLQSMVQYLSARLGEAGSWPRDPDNVLLLLDKLIERNIVEPDAARRKSISIILE
                     YAQYLVPAGEVAGLARTQGTNLVRLLGWAQNPYIKRLNLAFCLVADKLTEINDRLVRN
                     PHVATIEIPLPDASARESFASWVCETERISPSKNFTAKMLADQSSGLSLVSLNTVLSQ
                     SRYTAEGIDPARFRNLKKAMIERQCQGLVEFVEPKHKLDLVVGQAVARKRLENDAELI
                     TRGRFESAPMGYLLCGAVGTGKTFLAECYAGTIGIPCLKLRNFRSKYVGETEGNLEQV
                     LTVLRSLGPVVVIIDEADAALGSRSAEGDSGTSSRVFSMIAGQMGDTRYRGRILWMLL
                     TCRPDLLPIDLKRQGRAEVHIPMFYPQSEAEIREMFLAMARKNGISLSPEFVPLVNAD
                     RRLSGADIESIVLAAARRSACAGRDQPLAEDIQQAIDAFIPSAQGLEKELQELAAVLE
                     CTELEFLPPDWRAKVTEPNGRVKLQERMMAIRSLLED"
     gene            complement(4299..4675)
                     /locus_tag="P5316_02220"
                     /pseudo
     CDS             complement(4299..4675)
                     /locus_tag="P5316_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(4675..5430)
                     /locus_tag="P5316_02225"
     CDS             complement(4675..5430)
                     /locus_tag="P5316_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HRR83802.1"
                     /translation="MIIGKLWHTLKAQLNKLANFFWTADPIAQMQYEYDVAVNQLKEG
                     REGLEQYRALVERVTRQVASDKSHVSSLEAKVRAYLQAGDRETAARFALELQKAKQEL
                     AENEQQLKLHEEAYNNNLTKIKHAGNKLAKVREKIARYDAELKMSRAEAEMAKLAQSF
                     DFDVTTDFGQIEQVIQDKISLNRAKTRVAADLSSQGVEDVRREQAMEKALADQALRDF
                     EVQMGLVTPETTGVAEAAKELGPAKEKQAMSNE"
     gene            5846..6883
                     /locus_tag="P5316_02230"
     CDS             5846..6883
                     /locus_tag="P5316_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83803.1"
                     /translation="MAVSTDTLAGAWAAKAQVLAEWTAAHMVNRTDQWAQYLPPEQRS
                     YSRIVRIAPPKQLRGQACLTTDLLARHYVGDNVGHLIGLHAKGVDNSARWISIDLGQH
                     DPSAMATPAANLKAALAWYDTLKEQGFHPILEDSSGRGDYHLWVIFGDKVDAGTAWVF
                     ARTLVSNYADLSLPVPPKVYPRRAQVESASFKDWVRLPGRHHSHNHWSRIWDGGRWLE
                     GADAANAILQTRIDPGHFSLRSAVPPPQVTPPDITAASVEEQVTEVFQLPPVSASLSG
                     WSGQDEAAVAATPTQAEPDGASASMPPVPGAGSPEDDLIMIIRAWPSLPPVVKSGIVA
                     MVRSAQTHRGS"
     gene            complement(7061..7519)
                     /locus_tag="P5316_02235"
     CDS             complement(7061..7519)
                     /locus_tag="P5316_02235"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR83804.1"
                     /translation="MSGKAADDAPKRSGTRDDKNLFLDHLDRLDQRSTPLSVAERRRY
                     ERHRHRVDQGLEVIFDAFDEQPVTCHVRPRNLSQGGIAFLHEAYVYPETRCTVVLISA
                     TDQPIEVSGRVVGCRHISGKLHEIAMAFDRPIDAEDFRECIDEAVGGDES"
     gene            7810..8841
                     /locus_tag="P5316_02240"
     CDS             7810..8841
                     /locus_tag="P5316_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR83805.1"
                     /translation="MAFNFWTRSKTTSRGGVAEEPAPPVGVIPRPDDYDPAPQNVFED
                     DPLLAMFQQERFNLIFQQKAKWESHRDWSEVVQQATKYLDRQMAIVPEGRASLLRTLT
                     EVPDAEVLDFSVPSFLIDIHAVTNARYQLFVDAGGYDDLSLWPEAVWTHLIQFRDQTG
                     RPGPRYWRDGRHDQRLASHPVVGICWYEAAAFAKWIGQRLPTEPEWQLAASWRIVSDT
                     DVLYRFPWGDAMDRRKCNIWASQVGTTVPVDEYDQGAAPNNVLQLIGNVWEWVETDLD
                     IVTEEGVQVVGEMTMKAARGGAFDTYFESQCTALFRSGFNALTRAHNLGFRCALSLAD
                     ACWLGQSTE"
     gene            8850..9743
                     /locus_tag="P5316_02245"
     CDS             8850..9743
                     /locus_tag="P5316_02245"
                     /inference="COORDINATES: protein motif:HMM:NF040097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83806.1"
                     /translation="MYDEHGYRCNVCLICDHKNAPSAVICSQCGAPMALIEDARQQNR
                     KPAIITLLGEANVGKTVYLGFLLDLLSKRAGGFEAIPKGAYSVNLQQTVIGCLNSRRF
                     PPKTPLEVEHWKWAYYQVRHRLKRSDEWFDVLLPDMAGEALSQEVESPKTYAAISGLL
                     AKSAGAMVLIDAAVAASGSPYPDYFAYKLISYVDQRFATHRKERVRNPIAIVLCKADT
                     CPECFDNPREFAETNLVRLWNLCDSRFENVEFFAASVVGSLGYAHDEDGNVVQVPLHA
                     APRGVLEPFEWISQRMKAVQL"
     gene            9707..11224
                     /locus_tag="P5316_02250"
     CDS             9707..11224
                     /locus_tag="P5316_02250"
                     /inference="COORDINATES: protein motif:HMM:NF040416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83807.1"
                     /translation="MDLPADESRPVVRPAKAEPTTADRVVCEQAVYTCLCNAAGQGYR
                     IIAASGGLSPRDCGEITRYTPAMGGLCADTPEATGVSFFPISDGRFAVLYTCHAGKEP
                     TGRGGRRTYTRIFVVRGDDLRRFRNNPFALVRAAEAEGGTTVDLTAEVKLPPLLLSPE
                     RGLATPAFEFAGAAVPGRWIAAATDRLLSGNAVAFSAGVEGTALAELILLTIPAPIRP
                     EISFSVGVTFTVSRPHRLVILANVVAGTRERIAGTEYVFLDASGDPESAPAVNHPWAR
                     AVDRFIEAGRTSALIGMLADRFGEKDVKALERIGELCLALDSAASNSLENLIDIAMPY
                     VGLSMDLPLERELARQVVRLVRHRFSSLISHADCGQLSNVWRRMLGAEPRAPEAARLV
                     EESAALAVTRMSCLDPVQAMRMVLEHGLTLSAHRTATDLEYARRTVMACVAAWIPQAS
                     EAELTDAVGVLEDWRLRHPEDPAATQNLEQLRLRMSETAQAADSDEPAEVGVAAE"
     gene            11439..13058
                     /locus_tag="P5316_02255"
     CDS             11439..13058
                     /locus_tag="P5316_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR83808.1"
                     /translation="MSEAGSSVADLQAEIAALKARIRELERIEQAHVQAVEALRKSEE
                     RLRFIAERSPIVLFAIERDGTIILCEGKPLERLSLRSQDLVGRSVREVFLDAPQIVAD
                     FERVLSGETFTSPLSYRERSFEVWYSPVRDQRGEITSVIGVANDVTDRNRLQAQLLQS
                     QKLQSIGTLAGGVAHDFGNLLAVIIGNLSIVLRKDPVPAKVKDLLRDVMDAAERASAL
                     TRQLLAFARGGLQKPEPTNLNRHVESVLRIVRRTTPRRIELKADLVPDLPRIIADPAQ
                     MEQVIMNLCLNAVEASGQSGLIELSTVCEELDADQAAVLGLPAGRYVRLQVRDHGCGM
                     NEETVGRVFDPFFTTKPTGRGMGLAATQGIVHSHQGQIRIESVLGKGTVASVWLPVAP
                     WESSGRPAVAQAANAPPRGSETVLLIDQQTADLQATEAKLSSLGYCTVGRADLASALA
                     FLGTNSDDIDLVLLDAEISHLSRVSPVRLIRKHCPDAPILLMTRSPTDAWLRRLGKQG
                     AAGLLRKPFELAELATAVRSCLDAGKRKSDS"
     gene            complement(13720..14520)
                     /locus_tag="P5316_02260"
                     /pseudo
     CDS             complement(13720..14520)
                     /locus_tag="P5316_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(14697..15467)
                     /locus_tag="P5316_02265"
     CDS             complement(14697..15467)
                     /locus_tag="P5316_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR83809.1"
                     /translation="MVRQGQRILADITWHVKKGQHWALLGANGSGKTTLLKVITGYEW
                     PTTGSVTVLRQSFGKCNLPEFRKQVGWASSAIESRLPVYERAIDIVASGFFASIGLYR
                     QPSEAEWLTATEIIRHVGGDQIMHRPFGLLSQGEQRRIVIARALVNRPQLLLLDEPCA
                     GLDPAARESLLADLERLARSPHAPTIIMVTHHVEEIGPWIGHVLVLKNGTVLKAGPKN
                     ETLTTPVLSEALGCLCEVEKLGDRYRLTLIGGQSCSPT"
     gene            16026..16166
                     /locus_tag="P5316_02270"
     CDS             16026..16166
                     /locus_tag="P5316_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83810.1"
                     /translation="MKKVMFAIVSAATLFACGCCMPKIGCCTAGVLVDLLELLLGAMP
                     AA"
     gene            16421..16564
                     /locus_tag="P5316_02275"
     CDS             16421..16564
                     /locus_tag="P5316_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83811.1"
                     /translation="MKKVMFAIVSAATLFGCGCGCMPKISCCTAGVLVNLLELLMGAM
                     PAA"
     gene            complement(16782..17657)
                     /gene="argB"
                     /locus_tag="P5316_02280"
     CDS             complement(16782..17657)
                     /gene="argB"
                     /locus_tag="P5316_02280"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRR83812.1"
                     /translation="MQEAIAKAGSLIEALPYIQGYYDKIVVVKLGGSVMDNPETEREL
                     LRDVVFMNYVGMQPIIVHGGGKEINAAMDDAGLEPVFVQGLRYTDDRTLAIAEQVLCG
                     RINRRLVETLESLGVAAIGLNSLTSCVLFAEQMYLCGEENRRIDVGYVGKIERVNARI
                     LELLCEADTIPVIAPIARDRSGGKLNCNADTAAGEVAAAVKAEKLVMVSDTHGIRENP
                     KDPESFRPTLNRKEITDFMNRGIIDKGMVPKVQACLRALEAGTRKAHIIDGRIPHSLL
                     LEIFTDKGIGTQILQ"
     gene            18398..19984
                     /locus_tag="P5316_02285"
     CDS             18398..19984
                     /locus_tag="P5316_02285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR83813.1"
                     /translation="MSNWFDAVLVGLFMVSTAMLSVYGLHLYILLFLFHRRRSVKGAE
                     QRAFVDHYLANTPEREWPGVTTQIPIYNEADVATRVMEAVAAMDYPRGKHSIQVLDDS
                     TDETRLLVDRTARRLREQGVDIEVLHRDNRQGFKAGALKAGLAHCRHPLVAIFDADFV
                     PPKDFLRRAVPLLARDEKVACVQGRWAHLNQEESWLTKAQSVALDAHFAVEQGARAWN
                     GLMMNFNGTAGIWRKAAIEDPAVGGWSADTLTEDLDLSYRVQLAGWRIDYCLELACPA
                     ELPSTISALKAQQRRWATGGVQTAIKLLPRIWRSSLGLSHKIEATLHLTHYAVYLWTL
                     MLALLARPMLAMVDPLPFWWCFVFGWGLMILTVVASPIVYGYARYHLGGGLGGVRMTP
                     VMLLLGTGLSINNALAVIRGLYVRGGEFVRTPKSGSTRNARFTSRYKAQRFRRLWFAE
                     MLIGAYCSATWAIYVTAVHGPAFSVFLAIYAVSFLTIGWISRPEAGLSSRPRPLPEAE
                     AVAGPAVPARLAPANSVHVG"
     gene            complement(20057..21280)
                     /locus_tag="P5316_02290"
     CDS             complement(20057..21280)
                     /locus_tag="P5316_02290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR83814.1"
                     /translation="MAAQLAIHGGTPVRTKPFPSWPVYGQPEEQAVLRALRSGKWGKL
                     DGVEVAAFERDFARYQGARHGIAVLNGTVAIQVALMAAGIKPGDEVIVPPYTFLATAT
                     AVVTSNATPVFADIELDTFNIDPKAIEALITSRTRAIIPVHFAGLPADMDAIMAIARR
                     HNLVVIEDAAHAHAAEYKGRRAGSIGHMGTFSFQSSKNLTAGEGGFITTNDPALAEAC
                     REIHNCGRREGHAWYEHFVIAANFRLSEIQGALLNAQLQRLEEQARRRWENGRYLTEQ
                     LGQIPGIKPQKIIPECTRHAFHLYCFRIDAQELGAPRQAFLEALAAEGIPDFGGYPLP
                     LYKQRVFLDRAFGPFDGCRGIDYAKTCCPNCEKICYEQGGWLEHRMLLGTREDMDDIV
                     AAFRKVYENRSRLQG"
     gene            complement(21330..23120)
                     /locus_tag="P5316_02295"
     CDS             complement(21330..23120)
                     /locus_tag="P5316_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83815.1"
                     /translation="MSKADWLVVAASCALVFVVGIWFSRRSSKKGAEGYFTGNRDVPW
                     WAIGLANTATYQSGNGAFVMIVLVFGLAGNWIWWASWIIWMPLVAIVWARFWRRMRIV
                     TTAELISLRYGGKPAMIARKIYAFVCCFGFSVLIISYITGFFAQTIEPLCALNTSRIL
                     LIFGGTTVLYTMFGGLLGVVYSDVVQFAIMLLGSTLFFWLAVPQHGGWEQILARITAV
                     RPEALTQVPPTRSIDTLTVLILVLQGWFFAGSPTAGEGMTAQRFMSARNERHAIGGQL
                     FNCFLALTLRTLPLIGLGIIAMALFWTSASDAQLGPAPAGMSSLDKPAHAWGELIKRC
                     NLPVGFVGLLVAAEAAAYMSTLSSLTNWGGSFVVNDLLPSQNDPRRQVWVSRITTLLL
                     FVLAATITLLFVDDMVSWFKFINNAMVIFFLPLACFRFFWWRFNVWGELAAILLGLPI
                     EILVWFVLGYQEKPMWQGLGMLFVLSFVLLTIVTLLTPPESPETLKRFYKRCRPPGFW
                     GPVRGDTDLAGRHDPSSARLVFDSILGILACLGLVLATNAVFVKDWPVMIGGLAAAVV
                     LGGWLLARVLESGPADDPTPDASGLPRSST"
     gene            complement(23210..24829)
                     /locus_tag="P5316_02300"
     CDS             complement(23210..24829)
                     /locus_tag="P5316_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR83816.1"
                     /translation="MRQLSSAVFLLLGLIAATQTANAADLPNVLWISCEDVCPDLGCY
                     GDKYARTPNIDKLAREGIRYTHAFSVSGVCAPSRSAIITGMYPTTISTHHMRCKAVPP
                     ACVKCFTEYLRSAGYYCTNNSKTDYNFDAPVTAWDECSNKAHWRNRTPGQPFFSVFNL
                     TVTHESQIRCSPEQFARHMQKVAPEHRHDPAKAVLPPYYPDTPIVRNDWARYYDLITA
                     MDLQLADLLRQLEEDGLADKTIVFFFGDNGRGLPRAKRWLYDSGIHVPLVIRLPDRKD
                     AGTVCDDLISFIDFGPTVLSLAGVKVPAHMQGQPFLGGQRAGPRQYVFAARDRMDETY
                     DIIRSVRDKRFKYIRNFKPGRPYAQYIDYMEKMPTMQEMRRLNKEGTLTGPRKTFFLP
                     EKPEEELYDCINDPHEITNLVNLPEHRERLIEMRKVLEKWMDDTRDLGLIPEEELNER
                     MRPGGRWAVTAAPVMEPPGGSHRGPASITIFCPTEAASIAWTCEEGEQAHWRLYCEPI
                     RLSHTATVRAKAVRLGYKESPEVRAEYRIEQ"
     gene            25005..25688
                     /locus_tag="P5316_02305"
     CDS             25005..25688
                     /locus_tag="P5316_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83817.1"
                     /translation="MERFIDKHQAQITGTISCFDRILFKGHLPLGRPEAMEQFIARQG
                     TLIKDFGAFVTRQSEKVKRHAQATARRAGRPFIHPRRTVFLRTASQAHCDSETFARAA
                     QASGREVLTSRGACPLDFLGRTKAALKNDLNEFDGAAGLSRSGRRANDHEPAGQFVID
                     HLEGSIIPLSVGGCTNDRSCPFGGAGNEVDDGPSARQRSPSPGVRRGDPECPNPNGEH
                     PGRNDRNRL"
     gene            25802..27220
                     /locus_tag="P5316_02310"
     CDS             25802..27220
                     /locus_tag="P5316_02310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRR83818.1"
                     /translation="MMYQAGITTLRKIIVRAAFIGLFVSSLPAVAQAPSSAQEAPPRM
                     PAAVQHAPSVMPDRIVLTWTGDTTTTQAVTWRTSTEVSLAYCEVAEAEAGPQFPQRAR
                     RVNAVTRVFRCDLSKCHMHRVEMTDLKPATRYVYRVGDGENWSEWFQFRTASAAPERF
                     SFIYLGDGQNDLHSMWSRVVRQAYSDAPRAAFTLHAGDLVTSAESDALWGEWFAAGAW
                     INATVPVIATPGNHDYASTKNQDGTSRRWLSRHWAVQFAFPANGPEGLAESVYYIDYQ
                     NCRIISLNSNEKQEEQVGWMDKVLSENKKTWTVITFHHPIFSTAKGRDSTPLRELWKP
                     VFDRHGVDLVLTGHDHTYGRSGLVAPETNVTTGSGERSISGGTVYVVSVSGPKMYDLS
                     PASRAEVRRSAEDTQLYQIVSIDGPVLRYEARTATGDLYDAFTLKKRAGQLNEMIEQV
                     PNVPERRRPAETAKQRRRNGAG"
     gene            complement(27328..27969)
                     /locus_tag="P5316_02315"
     CDS             complement(27328..27969)
                     /locus_tag="P5316_02315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR83819.1"
                     /translation="MNMEPDYFAKAAATWDNEPRRIALMKAVGEAILREVRPTREMDA
                     LDYGCGTGLIGLFILPHVRSVTGADSSSAMLNVLRKKIAESGVSNMSVIKLDLQHDPL
                     PAERYHLIVTNMTLHHVADADAVMRAFCELLWPGGMLGIADLDTEPGIFHPPHAAGSV
                     HHNGFDRGELKIRLEEIGFEETRDTTAHTIRRPVQDGSEHEFPVFLITARRPC"
     gene            complement(27982..28482)
                     /gene="tsaA"
                     /locus_tag="P5316_02320"
     CDS             complement(27982..28482)
                     /gene="tsaA"
                     /locus_tag="P5316_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRR83820.1"
                     /translation="MTLEARPIGIIHTPFIQPAGTPIQPVYAAYAEGTVEVFKPFAEG
                     LADLEGFERIWLLYWCHCSAAPRMRVIPYRDTQERGLFATRAPARPNPIGLSCVRLLR
                     IEKDVLHVCELDILDGTPLLDIKPYVPAFDSFPDAGSGWLTEERTGRTIADDRFHVDK
                     NPTDGR"
     gene            complement(28722..29396)
                     /locus_tag="P5316_02325"
     CDS             complement(28722..29396)
                     /locus_tag="P5316_02325"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRR83821.1"
                     /translation="MCCENKLPLIGEKAPSFKAETTQGPITFPDDFKGKWVVFFSHPA
                     DFTPVCTTEFMTFACMQPDFEKLNCKLLGLSIDSTYSHIAWLRTIKEKIDYKGMKGVE
                     IDFPVISDLTMEVSRAFGMLQPAASNTQAVRAVFIIDPQAIVRAILYYPLSNGRNMDE
                     IKRLLIAMQTSDKHNVATPANWQPGDDVIVPPPGSCGTAKERVEKAASDTRVLDWFMT
                     LRKCPK"
     gene            complement(29513..30346)
                     /locus_tag="P5316_02330"
     CDS             complement(29513..30346)
                     /locus_tag="P5316_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR83822.1"
                     /translation="MLSITVLVENTANRLNLLAEHGLAFWIERDGRRILFDTGQGLAM
                     QHNAAVLGIDLSAVDEIALSHGHYDHTGGLAAALSNFRQAKVYAHLAAFRTRFVKDHD
                     GQVRPVGAPVESRDWLSSRVGQMIATRGGPVGLGGGIWLTGEIPRRNEYEDTGGAFYL
                     DEACTAADLVIDDQAMFIETTKGLVVLLGCAHAGVVNTLEHIRAATGEARIHAIMGGM
                     HLLAAGERRMAETAARLSSLNIEHVGLGHCTGFAAMARLHRELPSRCFHCVTGTRLVF
                     D"
     gene            complement(30402..30995)
                     /locus_tag="P5316_02335"
     CDS             complement(30402..30995)
                     /locus_tag="P5316_02335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRR83823.1"
                     /translation="MSATTDVSRRCHPSCLVCGSNRTDGLALHFQQQTDGSVVAEFHC
                     GAAFQGYPDRLHGGVVAMLLDAAMTHCLFAQDVAGLTAKLSIRYHRAVVLGVPVVVRA
                     RLVDAKAPLYYLESQVIQDGALCATAKGAFWDSACGDLVRDASAEAAESSQACFRSVT
                     IRPRSKFLFPLSGGNADARPATARPETHLGTGMMPVQ"
     gene            complement(30979..31317)
                     /locus_tag="P5316_02340"
     CDS             complement(30979..31317)
                     /locus_tag="P5316_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRR83824.1"
                     /translation="MKIAVPVANGRLCPHFGHCSQFVIHEVDPATRRTIRREYLTPPP
                     HEPGILPRWLHEQGVNAVIAGGMGQRAQQLFAQSGIEVIVGAPDRDPDEIVAAYLAGS
                     LEAGENVCDH"
     gene            complement(31372..31734)
                     /locus_tag="P5316_02345"
     CDS             complement(31372..31734)
                     /locus_tag="P5316_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRR83825.1"
                     /translation="MKVVVTAQGPTADSPVDPRFGRAQYLILADTETGECTAFENVAN
                     LNAVQGAGIQTGKRVVDLAAKAVITGHVGPKAFSTLSAAGIEVYTDASGTVNQAIEQF
                     KAGALQRAQGADVKGHWA"
     gene            complement(31907..33277)
                     /locus_tag="P5316_02350"
     CDS             complement(31907..33277)
                     /locus_tag="P5316_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRR83826.1"
                     /translation="MQSTYARSQEVILDSINEGVFTIDLDWRITSFNQAAERITGVDR
                     QDAMGKPCCEVFRASICEGSCALRRTLSTGKPVVNATAHIVNQSGQRIPIRLSTAILK
                     KPDGTVIGGVETFQDLSQIEQLQKQLTARYTFEDIVGRSPAMVSLFELLPRIAESDST
                     VLIEGESGTGKELFARAIHNLSRRRRKRFVAINCAALPDTLLESELFGHKAGAFTDAR
                     RDKPGRFALADGGTVFLDEIGDISPAMQVRLLRVLQERRIEPLGSVEPIKVDVRVVAA
                     TNKDLLSLVRAGKFREDLFYRIRVVYLKLPPLKQRREDIPLLIDRLIAKFSRLQGKDI
                     AGVSPEVLARLMEHDYPGNVRELENIIEQAFVLCQGGLIELHHLPPELRPVTSQAGGE
                     HGTPTTLKSMERLLIAEALRRHHGNRKRAAADLGINPSTLFRKLKALKIESPPTDGRS
                     RPHMAK"
     gene            33486..33887
                     /locus_tag="P5316_02355"
     CDS             33486..33887
                     /locus_tag="P5316_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRR83827.1"
                     /translation="MKIAIPQWQDRVSPVFDVAGNLLVVDVANGREEARLKVDLTATE
                     PLRRAQQVAGLAPDVLICGAISWPLEMALTSAGVRVISHICGGIDEVLEAFANGNLAD
                     ERFIMPGCCRRRHMRGRRYRHGGLGGPRGVR"
     gene            34051..34410
                     /locus_tag="P5316_02360"
     CDS             34051..34410
                     /locus_tag="P5316_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HRR83828.1"
                     /translation="MPAGDGTGPMGMGPMTGRAAGYCAGFGVPGFANPLPRMGWGRGG
                     GWGRGGRGWRHWFYATGLPFWARMPVPGAVSPMAAEPTTEYQTQVLKAQAEQMEKTLE
                     QIRKRIADLESMQSEAR"
     gene            34586..34978
                     /locus_tag="P5316_02365"
     CDS             34586..34978
                     /locus_tag="P5316_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83829.1"
                     /translation="MRTALWTLFCSGLVLAAYGRPTGVLDPSDSNTLLIFHNGTGPMC
                     IEALNWLAGIQAEEPDLVVEQHLTTDPASLALLGQLRLQYGQSQGVSTSFEYLPFIFF
                     RGEAFSGFSEGVKQSLTWLIESMDASAS"
     gene            35293..35472
                     /locus_tag="P5316_02370"
     CDS             35293..35472
                     /locus_tag="P5316_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR83830.1"
                     /translation="MAVVNRARCRACGLCVSACPREAIRMARCAEVDASLCSGCGACV
                     DVCPVEAISLQPRES"
     gene            35539..36465
                     /locus_tag="P5316_02375"
     CDS             35539..36465
                     /locus_tag="P5316_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HRR83831.1"
                     /translation="MQTYLDCVPCFVRQALDSARHLTGSPEVHERLLREVLALASTMS
                     FDSPPPVMGRLIHRRLRELTGQADPYEAAKRKANEFALRLYPDLKHVVISSRDPFAAA
                     VRFAIGGNVIDLGVKTGLTESDIRSAIREAIEGQVDPDAVAALRQTAMPAGDILYLAD
                     NAGEIVFDRLLIEQMPPGSVTVVVKAGPIINDATREDAEAAGLADFVEVIDNGDDAPG
                     TILDRCSPAFRKRFDAAELIIAKGQGNYETLNECGHRNLFFLLKVKCGVIARDLQCRL
                     GEMAVRKHGVVSQSPLKRPGDDFDRLAEEAHS"
     gene            36572..37219
                     /locus_tag="P5316_02380"
     CDS             36572..37219
                     /locus_tag="P5316_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83832.1"
                     /translation="MLRDIRIQLKAHVPFTAFGTATGIAIMALIVWTGVSQSTSSALF
                     WTFHPIHVFLSALATAAMYRLHSGGKLLAIVLVGYVGSIGIATLSDSVIPYVGEYLLG
                     LPNRGVHFGFIEKWWLVNPLALLGIAVARLWPQTKFTHSGHVLLSTWASLFHMTMALG
                     DNVTALTWALLPVFLFLAVWVPCCTSDIVFPLILARRRLPVDAPREQSEEAYRTS"
     gene            37216..>37768
                     /locus_tag="P5316_02385"
     CDS             37216..>37768
                     /locus_tag="P5316_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR83833.1"
                     /translation="MRIAIASGKGGTGKTTVATNLAYVASLDGRTVAYVDCDVEEPNG
                     HLFLKPEWLVSKPVTRPVPQVDETRCTQCGRCGEICQYSAIVPLGTTVLVYPELCHGC
                     GGCKLVCPAGAITETPRQIGVLEMGWSGDIQFNRGVLNIGEAMSPPLIRQVKSAPPKT
                     DLTLIDAPPGTSCPVIASVRGADC"
BASE COUNT         6922 a        11175 c        12004 g         7667 t
ORIGIN      
        1 cggcggccat caactcggcc actttcttgc ggttcgtctc ctccttgaga tcgaccatcg
       61 cgtcgaagat cccgcgaaca aggctttcca tgatccccgg gttgtcctgg gcgaagtcgg
      121 cccgggcgaa ccacacatcg gcgatcagct tgttggcggt cgcggtggtc accagcatgc
      181 ggtttccctt gaccttctcg aggttgtaga tgtccggggc ccatgagacg gcgccggcga
      241 tgtctttctg ggcgttgaac gcggccgccg cctggaacgc gtcctcggtg tagatcatgt
      301 tcacctcgga aggctgcacg ccgccggaca ccagcatatt cagggcgaag tactgagagg
      361 gcgaattctg ggccagcacc agcttcttgc cacgaaggtc ggatacggtc ttgatgtagt
      421 cgcggacgac gattccgtcg ccgccgttgg accagtcgac ctgctggtag attcggggca
      481 tgacgcggct gtcgcgcggc ttgccggtga catcaacgaa gccttccatg aacagcggga
      541 ccatgtcgag cgtggcccat ccgatgtgga cgtcgccggc ggcgtaggcg tctcgcatgg
      601 ccacggggtt gtcgatcagc accagttcga ccttgaagtc cttgccgtcc ggcgtcttcc
      661 agacctttcc cggcctgaag ccgttgttgg cgtagatgat cggcgcccag ccggcccaga
      721 cgttcaaggc gaatcggacg gtgttgtcgg tcatcggctt ataagccgag gtccccttga
      781 cctggggcag gcgctcgacc ggcttgaagg tgtactcttt gaccgtggtg acgcccgtcg
      841 ggttgctggc atcctcgacc tgctcgccgc ccggcaacaa agccacatcc gtgccgggcg
      901 ttttttccct gggagcaaac aagtcgctgc gatagatcgc gaaggccacc aggccgacga
      961 tgatgatgcc cagcaccgcg tagaacggtc ctttaggttg tcctgccatg gttctacctc
     1021 ccatctgggt ttttttgccg gctcaccgct gatgatgagc cggcggttcc catgcgtcat
     1081 tgtctcgctt tgcggcgcca gcatctccgc cgccaggttc gcggtcaata gctgccggtc
     1141 ggcctactcc ttttccgcag gcgcctcggc cagaatccgt ttctcataaa tgtccgtcag
     1201 ccgggcccgg agttgtgctc cgtcggcggc ttcggtcgta tgtccgttca cctcgctgag
     1261 aaagccgaac ttagtcgccg aggtgtcaaa ggcgatgaag tgcatctcca cctcgccctg
     1321 ggtctgggca tgtagttgcc ttgcgacgcg gtcgggcggg cggccggcgg tgttctcgcc
     1381 atccgtgatg catatgagat acttgcgcac gcagccggac tggtagaggg ccttgaaacc
     1441 ctcctcgatc gcctcgccga tcgccgttcc tccgccgggc tggggaatcg tctgcagagc
     1501 ctgttgcgtc ccggccaggt cgaatcgtcc catccgcagg accgaggaag gcgaactgct
     1561 gaagttgaaa agggccatct cgaggacgcg gtccgtgtgg gttttctccc actggcccgt
     1621 gtagtcgacg atttccttca aggcgtcgcg agcgatgaga tacttgggcc gtcggacgcc
     1681 ctgatggtcg acaacctcct ccttcatact gccggatgtg tcgacgagga tggcgacggc
     1741 ggtgcataac cgctgatcgg cctgcggcag gggaatcaga tgagtggcga cctcagcggt
     1801 catcggagcc ggcgatcggt gcggcgaggt cggaggtggg gagcggtcgc agccgtacag
     1861 caacagctcc aggctcagaa cgaacaagcc tgcctgccag ccgccgcatg caagtcgttt
     1921 cgttaccgga ttgttgcctg tcacggtctt catggggcgg cttcctttca gaacaggttt
     1981 tgaccgaccg aataaacctg cgtgccggtt tagccggccg gctccagcgg ggcaatgata
     2041 aatccgccgc tttttttgaa tcgacctagc cctggaacgt cgcctgaggc cggtgttcct
     2101 gcatcgaatg cagcatctgc atcaggtcat atgcggtcag ttcgcacagc agtcgggtga
     2161 ttttctcatg cagcgcgtcg ctgagctgcg gctcgatctc cttcatcaat cggacgagcc
     2221 gcttttcctg ggccgcaagc tcggactcct cgatcctgcc gtcggcaacg gcctccatga
     2281 aggaggtcag cttgcgggcg tactgatcga taagcggctt gtgagagttc gcatcgagcc
     2341 agctcgacga cctggacctt gccgccccgg ccgtcggggt gcggggctta cgctttttcg
     2401 ggctgctctt ggcggctcct ttcttcgccg gccgggcctt cttcctcttg accatcgctt
     2461 tcttcttggc catacgtgtc tcctttgtgc tcaatcctcc agaaggcttc gaatcgccat
     2521 catgcgttcc tggagtttca cccgtccgtt gggctcggtc accttcgcgc gccaatccgg
     2581 cggcaggaac tccaactccg tgcattcgag caccgccgcc agctcctgca gttccttctc
     2641 gaggccctgc gcggagggaa tgaaggcatc gattgcctgc tgaatgtcct ctgccagcgg
     2701 ttggtcgcgg cccgcacacg ccgatcgtcg tgcggcggcc aggacgatgc tctcgatatc
     2761 cgccccgctc agtcgccgat cggcattcac cagcgggacg aactcgggcg acagggagat
     2821 tccgttcttc cgggccatcg ccaggaacat ctcgcggatt tcggcttcgc tctgcgggta
     2881 aaacatcgga atgtgaacct cggcgcggcc ctgtcgcttc aagtcgatgg gcagcaggtc
     2941 cggccggcag gtgagcagca tccacagaat ccgaccgcgg tatcgcgtat cgcccatctg
     3001 gccggcgatc atcgagaaca cgcggctgga cgttcccgaa tcaccctcgg ccgagcggct
     3061 gccgagcgcg gcatcggcct catcgatgat caccaccacg ggccccaggg accgcagcac
     3121 ggtcagcacc tgttcgaggt tgccctcggt ttcccccacg tacttcgacc ggaagttccg
     3181 cagcttgagg cagggaatgc cgatcgtgcc cgcatagcat tcggccagaa aagtcttgcc
     3241 cgtgcccacc gccccgcaga gcagatagcc catcggagcg gactcgaatc gtccgcgggt
     3301 gatcagttcg gcatcgttct ccagccgttt gcgagcgacc gcctggccga cgaccagatc
     3361 cagcttgtgt ttgggttcga caaactcgac aagaccttga cactgtcgct cgatcatggc
     3421 cttcttgaga ttgcggaatc tggcggggtc gatgccctct gcggtgtacc gcgactgcga
     3481 gagcacggtg ttgaggctta ccagactcag cccgctggac tggtccgcca gcatcttcgc
     3541 ggtgaagttt tttgaggggg agatcctctc cgtttcgcac acccacgacg cgaatgactc
     3601 gcgagcgctc gcgtcaggga gggggatttc gatcgtggcc acgtgcgggt tgcggaccag
     3661 ccgatcattg atctcggtga gtttgtccgc gacgaggcag aaggccaggt tcagtcgttt
     3721 gatgtagggg ttctgggccc atccgagcag cctgaccagg ttggttcctt gggtgcgggc
     3781 cagtccggcc acttctcccg ccggaaccag atactgggcg tattccagga tgatgctgat
     3841 gctcttgcgc ctggccgcgt ccggctcgac gatgttgcgc tcgatgagct tgtcgagcag
     3901 cagaagcacg ttatcggggt cgcgcggcca ggaaccggct tcgcccaacc gggcactcag
     3961 atactgcacc atggattgca ggcgttgcgg gtcgctgccg gccaacggcc gcagacctcg
     4021 gcccaggtcg tattgcagca caatgtccca cgtgccaaaa acctgggttg cgaggaattg
     4081 ggacagattg acgtatcctt cgccatcgcg cgtcgggcag cgcacgaggt cgtggacgtt
     4141 cccatggagg acgaacaggc aggttgtgcc cgaaaaatac aggtcggcca actgctttgc
     4201 ccaatcagga taccaggccg ggagcgatga gcgatgagtg atgagtgatg agtcatgcat
     4261 gatgggtgat gggtcaggag tgggtgaacc ggtctgagtc atgccgaatg ccgccttcgc
     4321 tcgacggaag atcgaagccc tccaaccagg cgtcccactt cctgggccct gcccatcaac
     4381 agcctgaacg tcgcctcatc gatgtagtcg gcgtctctcg tcacgtagag gagagatcgc
     4441 acctcggcgc aggagccctt cgcaaccgac aaaaactgat ggaattctcc cgatcgcccc
     4501 ctctcaaacc cctccgcgat attggacatg atcgagacgg ccgcgttcct catctggtcc
     4561 ctcaagccaa aatcgcgggc aagggcacgg tgtccggtca gcttatagat gtcggccgcg
     4621 aacgagtctt ctgccacgcg atgaaatcct caaaccgctc aatcctgcca ctcatcactc
     4681 attactcatt gcttgttttt cttttgccgg tccgagttcc ttggcggcct cggccacccc
     4741 ggtcgtctcg ggggtgacga gtcccatctg aacctcgaag tcacggagag cctgatcggc
     4801 cagggctttc tccatggcct gttcgcgtcg aacgtcctcg acgccctggc tggagaggtc
     4861 ggcggccacg cgggtcttgg cccggttcag gctgatcttg tcctgaatga cctgttcgat
     4921 ctgcccgaaa tcggtggtca cgtcgaaatc aaaggattgg gccagtttgg ccatctcggc
     4981 ctcggcccgg ctcatcttca actcggcgtc gtagcgggcg atcttttccc ggactttggc
     5041 cagcttgttg ccggcatgct tgatcttggt caggttgttg ttgtaggcct cttcgtgcag
     5101 cttgagctgt tgttcgtttt ccgcgagttc ctgtttggcc ttctgcaact cgagggcaaa
     5161 gcgtgccgcg gtctcacgat cgcccgcctg gagataagcc ctaaccttgg cctccaagct
     5221 ggacacatga gacttgtcgc tggcgacctg gcgggtgact ctctccacca aggcgcggta
     5281 ctgctccagc ccttcacgtc cttccttcaa ctggttgacg gccacgtcgt actcgtactg
     5341 catctgggcg atggggtcgg cggtccagaa gaaattggcc agcttgttca gttgtgcctt
     5401 gagggtatgc catagtttgc caatgatcat ttcacacctc gcgtctttcg gccggaacgg
     5461 acgggtacgg tccggactcg gtggcgtttt cttgtccgcc gaacgggcag aaccctgcgc
     5521 accggtctgg tgtggtcgtg tatatcgggc cgtggtagtg ctgtcaatta tacgttcggc
     5581 acattgttat aaggcttgcg ggaggagatt attacccgga tgcaccatgg tatttcgtcc
     5641 aggctcaggt tccgaggcgg ttttcgccgt gcagttgcta agggggggtg aggggccggg
     5701 ccgatgtatc gtaagatcct gagggaccgg cgggccggtt ccgtatcgcc aggctttggg
     5761 atttatcggg ttaagaggca cggggggcgc atgtgacggc cgggctccgc cacccggttg
     5821 ccgggtgtga ccccaacggt gatgaatggc cgtttccact gacacacttg cgggagcatg
     5881 ggcagccaag gcccaagtgt tggcggaatg gaccgcggcc cacatggtga accgcacgga
     5941 ccagtgggcc cagtacctgc cgccggagca gcgcagctac agccgtattg tccgtattgc
     6001 ccctcccaag cagttgcgcg ggcaggcctg cctgaccacc gacctgctgg cccgtcatta
     6061 cgtgggagac aacgtcgggc atctgattgg cttgcacgcc aagggcgtgg acaacagtgc
     6121 gcgctggatc agcatcgatc tggggcagca cgacccatcg gcgatggcca ccccggccgc
     6181 caatctgaag gcggccctgg cctggtatga cacgctcaag gaacagggct ttcatccgat
     6241 tcttgaggat tcaagcgggc gaggtgatta ccacttgtgg gttatcttcg gcgacaaggt
     6301 agacgcgggt accgcctggg tgttcgcccg cacgctggtc agcaactacg cggatctgtc
     6361 gttgccggtt cctccgaaag tgtatccccg gcgtgcgcag gttgaatccg cgagtttcaa
     6421 ggattgggtc cgattgccgg gtcggcacca cagccacaac cactggagca ggatctggga
     6481 cggcggcaga tggctcgagg gggccgatgc ggcgaacgcg attctgcaaa caaggataga
     6541 cccgggccat ttttctttgc gatccgcggt tccgccgcca caggtgacgc cgccggatat
     6601 cacggccgcc agcgtcgagg aacaggtgac ggaggttttc cagttaccgc ccgtttccgc
     6661 atctttgtca ggctggtccg ggcaggatga ggcggccgtc gcggcgaccc caacgcaggc
     6721 tgagcctgac ggggcgagcg catcgatgcc gccggttccg ggggccggct cgcccgagga
     6781 tgacttgatc atgattatca gggcgtggcc cagtctgcct cccgtcgtca agtcgggtat
     6841 tgtcgccatg gttcgcagcg cccagacaca ccgtgggtcc tagccggcac tgccttttcc
     6901 agtatcgcgc gataccgatg tccagacgga tgcggcgccg gccagatacg cccgcgcggc
     6961 gggcaggttt gcagcgaacc cgaatgtcgg aacgaccctg cggcggcagg accagtgggc
     7021 cggcaatgcg ggagtcgggc tggatcgaag tccgaggtga tcaggattca tcgccgccga
     7081 cggcttcgtc gatgcactcg cggaaatctt ccgcgtcgat ggggcggtcg aatgccatgg
     7141 cgatttcatg cagtttgccg gagatgtggc ggcagcccac gactcgaccc gacacttcaa
     7201 taggttgatc ggttgccgaa atgaggacga cggtgcagcg cgtttcgggg tagacgtagg
     7261 cctcgtgaag aaacgcgatc ccgccctggc tgaggttacg aggccgaacg tgacaagtca
     7321 ccggttgctc gtcgaaggcg tcgaagatga cctcgaggcc ttgatcgacg cggtgcctgt
     7381 gccgctcgta ccggcggcgt tcggcaacgg acaggggtgt tgatcgttga tcgagacgat
     7441 caaggtgatc gagaaacaag ttcttgtcgt cccgtgtgcc ggatcgcttc ggcgcatcgt
     7501 cggcggcctt accgctcatg gtggttttgt cctcgggtat cggcgaggtt ttcccggctc
     7561 gtttcgtatc gacccgcagg cagggtgact tgtgtgaacg gcctgccgtt gtccgataaa
     7621 tccgcctcac tcccgtcgtc cgcatggtca tagctgatgt accgcgattg tcacagccaa
     7681 gtctcaagca tcgtcgggcc aggaccgaat agcagataca ggaagcgcca cactccggta
     7741 atgcggcgtc gcatccggag ccgacgcgca gcggccgatc cgccggccga cgtatgaaag
     7801 agtatcttca tggcgttcaa tttctggacc cgaagcaaga ccacctctcg cggcggcgtt
     7861 gcagaagagc cggcaccgcc ggtcggagtc atccccaggc cggacgacta cgatcccgcc
     7921 ccgcagaacg tatttgaaga cgatcccctc ctggccatgt tccaacagga acggttcaat
     7981 ctcatctttc agcagaaggc caagtgggaa tcgcaccgcg attggtccga ggtcgtgcaa
     8041 caggccacca agtaccttga tcggcaaatg gccatcgtcc cggagggccg cgcctccctg
     8101 ctgcgaacgc tcaccgaggt tcccgacgcc gaggtgctcg acttcagcgt tccctccttc
     8161 ctgatcgaca ttcacgcggt caccaacgcc cgttaccagc ttttcgttga tgccggcgga
     8221 tatgacgatc tctcgttgtg gccggaggcg gtttggaccc atctcatcca gttccgcgac
     8281 cagaccgggc gtcctggtcc gcggtactgg cgtgacggtc gtcacgacca gcggctggcg
     8341 agtcatccgg tggtcggcat ctgctggtac gaggccgcgg cgttcgccaa gtggatcggg
     8401 cagcggctgc ccaccgagcc ggagtggcaa ctggctgcga gctggcggat cgtcagcgac
     8461 accgacgtgc tctaccgctt tccgtggggc gacgcgatgg accggcgcaa atgcaacatc
     8521 tgggcttcgc aggtagggac gacggtaccg gtggacgagt atgatcaggg ggcggcgccc
     8581 aacaacgtcc tgcaactgat cgggaacgtt tgggagtggg tcgaaaccga tctggacatt
     8641 gtcaccgagg agggggtgca ggtcgtcggc gagatgacca tgaaggcggc tcgcggcggg
     8701 gcgttcgaca cgtatttcga gtctcagtgc accgccctgt tccgcagcgg attcaacgcc
     8761 ctgacgcgag cccacaatct gggctttcga tgcgcgttga gcctggcgga cgcctgctgg
     8821 ctcggtcaga gcacggagtg aaagacggca tgtatgacga acacggctat cgctgcaacg
     8881 tgtgcctgat ctgcgatcat aagaacgcgc ccagcgcggt gatctgcagc cagtgcgggg
     8941 ctcccatggc cctgatcgag gatgcgcgac agcagaaccg caagccggcc atcatcaccc
     9001 tgctcggcga ggccaacgtc ggcaagaccg tctacctcgg gttcctgctc gacctgctga
     9061 gcaagcgggc gggaggtttc gaggccatcc ccaagggagc ctactcggtc aacctgcagc
     9121 agacggtcat cgggtgcctg aacagccgac ggttcccgcc caagacgcct ctggaggttg
     9181 aacactggaa atgggcgtac tatcaggtcc ggcaccggct gaaacggtcc gacgagtggt
     9241 tcgatgtgct gctgccggac atggccggcg aagccctgtc gcaggaagtt gagtcgccca
     9301 agacgtacgc agccatcagc ggacttctgg ccaagagcgc gggggccatg gtgctcatcg
     9361 acgccgccgt tgccgcatcc gggtcgccct acccggacta cttcgcctac aagctcatca
     9421 gctacgtgga ccagcggttt gccacccacc ggaaagagcg ggtccgaaac cccatcgcca
     9481 tcgtgctgtg caaggccgat acgtgccccg aatgctttga caaccccaga gagttcgccg
     9541 aaaccaatct cgtccgactg tggaacctct gcgacagccg cttcgaaaac gtggaattct
     9601 tcgccgccag cgtggtcggc tcgctcggat acgcccacga tgaggacggc aacgtggtgc
     9661 aggtcccgct ccatgcggca ccacgcggcg tcctggagcc gttcgaatgg atctcccagc
     9721 ggatgaaagc cgtccagttg tgaggccggc caaagccgaa cccactacgg ccgacagggt
     9781 tgtctgcgaa caagcggtct acacctgcct gtgcaacgcg gccggacaag gataccgcat
     9841 catcgccgcc agcggcggac tgtcgccccg ggattgcggg gaaatcacgc gctatacgcc
     9901 cgcgatggga ggattgtgcg ccgatacgcc ggaggccacc ggcgtgtcgt tcttcccgat
     9961 cagcgacggg agattcgcgg tgctgtatac gtgtcacgcg ggaaaggagc cgacggggcg
    10021 gggcgggaga agaacgtata cgcgcatctt cgtggtccgg ggtgacgatc tgagacggtt
    10081 ccgcaacaac ccctttgcct tggtgcgagc ggccgaggcc gagggcggaa cgacagtgga
    10141 tctaaccgcc gaggtcaagc tgccaccact gctcttgtcg ccggaacgag gactggccac
    10201 ccccgctttc gagtttgcgg gcgcggcggt cccgggaagg tggattgccg ccgccaccga
    10261 ccggcttctg tcgggtaacg cagtggcttt ctcggcggga gtcgaaggga cagccctcgc
    10321 cgaactcatt ctgcttacga tcccggcccc gatcaggccg gagatctctt tttccgtcgg
    10381 ggtgaccttc accgtttcgc gaccccacag actggtgata ctggcgaatg tggtggcggg
    10441 gacgcgggaa cgcatcgccg gaaccgaata cgtgttcctc gatgcctccg gcgacccgga
    10501 atcggctcct gccgtcaatc atccgtgggc ccgcgcggtc gatcggttta tcgaggccgg
    10561 ccggaccagc gccctgatcg gcatgctcgc cgatcgtttc ggcgagaagg acgtgaaggc
    10621 tcttgaacga atcggcgaac tgtgtctggc cctcgacagc gccgcgtcga acagcctgga
    10681 gaacctgatt gatattgcca tgccttacgt cgggctcagc atggatctgc cgttggaacg
    10741 tgaattggcc cggcaggtgg ttcgactggt caggcaccga ttctcgtcgc tcatctccca
    10801 cgccgactgc gggcaattgt cgaacgtttg gaggcgcatg ctgggagccg aaccgcgggc
    10861 gcctgaagcg gcccggctgg tcgaggagag cgcggctctg gcggtgacgc gcatgagctg
    10921 tcttgacccg gttcaggcca tgcggatggt gctggaacat gggttgacgc tctcggcgca
    10981 ccgcactgcc accgatcttg aatacgcccg ccggaccgtg atggcctgcg ttgccgcctg
    11041 gatcccgcag gcaagtgagg cggaactgac cgatgccgtc ggcgtgctgg aggactggcg
    11101 tctccggcat cccgaagacc ctgcggccac gcagaacctc gagcaactcc gtcttcgcat
    11161 gagtgagacg gctcaagcgg cggatagcga tgagccggcc gaagtcggag tcgcggcgga
    11221 gtaatgcgct cgcatccggg ggcaatcggc ccgtggcggg ctgatgacat cttttcggca
    11281 cgcgcgattt actctacgtc ttctttcatc tacgataggc gtacgttctg aagcggcgtg
    11341 gggtaccgtt gcaaccgtca ccggatcggc gggatggcgg gaccgcaccg ggattctgaa
    11401 acgcagcggg aacgatcgga tacttccgga gcgacgggat gtctgaggcc ggctcatcgg
    11461 tggctgattt gcaggcggaa atcgccgccc tgaaggcccg gattcgcgag cttgaacgga
    11521 tcgagcaagc gcatgtacag gcagtcgagg ccctccgcaa gagcgaggag cgactgcggt
    11581 tcatcgccga gagatcgccg atcgttctgt tcgccatcga gcgggacggc acgatcatcc
    11641 tgtgcgaggg caagccgctc gagcgtttaa gcctccggtc gcaggatctg gtgggccgat
    11701 cggtgcgaga ggttttcctt gatgctccgc agatcgtagc ggactttgaa cgagtccttt
    11761 caggcgagac gttcacttcg ccgctgagtt atcgagagcg cagcttcgaa gtctggtact
    11821 cgcccgttcg agatcaacgg ggagagatta ccagtgttat cggcgtcgcg aacgacgtga
    11881 ccgatcgcaa ccgtctccag gctcaactcc tgcaatcgca gaagctgcaa agcatcggga
    11941 ctcttgcggg cggcgttgct cacgacttcg gcaatctgct cgcggtgatc atcgggaatt
    12001 tgtccatcgt cctgcggaag gatccggttc ccgccaaggt caaggacttg ttgcgagacg
    12061 tgatggacgc cgcggaacgg gcctcggcgc tgacccgtca gctattggcc tttgcgcggg
    12121 gcggattgca gaagccggag cccacgaatc tcaaccgcca cgtcgaatcg gtgctccgga
    12181 tcgttcgtcg aaccactcct cgacggatcg aactgaaggc cgatctggtt ccggatctgc
    12241 ctcggatcat cgcggatccg gcccagatgg agcaggtgat catgaacctg tgccttaacg
    12301 cggtcgaggc cagcggacag tcgggcttga tcgagttgtc caccgtttgc gaggagttgg
    12361 acgccgatca ggcggcggtt ctcgggctgc cggcgggtcg ctatgtccgg cttcaggtgc
    12421 gggaccacgg ctgcggtatg aacgaagaaa ccgtgggcag agtcttcgat cccttcttca
    12481 ccaccaaacc caccggacgc ggcatggggc tggccgccac gcaaggcatc gttcacagcc
    12541 accagggcca gattcgtatt gagagcgttt tgggcaaggg taccgttgcg tccgtgtggc
    12601 tgccggtagc accttgggag tcttccgggc ggccggccgt tgcccaagcc gcgaacgccc
    12661 cgccgcgagg cagcgaaacc gttctgttga tcgaccagca gaccgccgac ctgcaagcca
    12721 ccgaagccaa gttgtcttcg ctgggttatt gcaccgtcgg acgggctgat ctggcctcgg
    12781 cgctggcttt tctgggaacc aattcagatg atattgacct ggtgctgctc gatgcggaga
    12841 tctcgcatct gtctcgcgtc agtcctgtga gactgatccg caagcattgt ccagacgcgc
    12901 cgattctctt gatgactcga tcgccgactg acgcgtggtt gagacgtctt ggcaaacagg
    12961 gcgcggcagg tctgcttcgc aagccgtttg aactcgccga attggccacc gcagtccggt
    13021 cgtgtcttga cgcgggcaaa agaaagagcg attcgtaggc ttccatcgga actggtgcaa
    13081 cgctcagacg gcctttgccg gccgggcgtt cctggcaggg ggggttgcag gttccgccgg
    13141 cgccggcggc aaagagggag gcacgacgga ggcctccaca acggcgtgtt cgctagcgac
    13201 gcccgcctgc acggcgtttt taccggttgg atctcccgag tcggaagcct tcaactgcgt
    13261 cgatcgaatc ttgcgcttgg gcggttcggg tcgggatgac gacggggagc aggacgattg
    13321 tgtcgaacca tagcgcggct cgacaacagg gaccggtttg gtctgggaag cggagtcgga
    13381 tgctccggcc gtcttggctc ttgccggttt gtgagaatgc agcacggcag tggccggttt
    13441 ggcgggtacc aggaccgcca acgcaggatc gattccgggc gtcttggttt gctgttgctt
    13501 gtttgagtgc tgcgcggccg ttctctgcac gtgagccggc cgccgcttca ccaatctctc
    13561 gggttggggc gcagttgcgg cgggagactc gggcttcgtt ttctcgggcg gctcagactt
    13621 cgcgggttgg atattcggca aagaggccgt tcctcgtgcg aagacctcgg cctttttcga
    13681 ctcagcaggc tcagagggct gcttcgttgc cgcgtttgtc tcgggcgagt caagttccgc
    13741 cgcaagctga aggtagtctt cggccccatg gctgtccgga gcgtattcga aaatgctctt
    13801 gccgtagctg ggcgactcgg ccaatttgat attccggcgg atgaccgtcc gaagcacgcg
    13861 tgtatcactc catggaacac cagtgccgcg ggcctgggca aagaatgacc gcacgtcgtc
    13921 gatcacttcg cccgccagct tggtccccga ctcgttcatg cacacgacca cgccggtcac
    13981 cttgagggcg gggttgatgc gtttgctgac caccgccacc gtttcgagca gcttgctcag
    14041 accctggagg gccagatagt gcggttggag ggggatcagg acttcggtcg cagcacacag
    14101 ggcgttgagg gtcagaatgt tcagcgacgg ggggcaatcc atcaacacat aatcataggg
    14161 agccgtcttg ctcgagttga tatgctgatc cagcagatcg cgaagaatga cctcccgtcc
    14221 cacgacgtta accagttcga cttctgcacc ggcaaggtcg atacttgccg agcagatcca
    14281 aaggttgctg gcgatcctgc gacgggcttt ggccaccgtg gctgagtcgg taagcatttc
    14341 gtagacgccg atctgaccgg aggtaggatc ctgtccgaga tggagggtca gatgcgcctg
    14401 tggatcgagg tcgatcagca ggacccgatg ccccatcctt gccagagccg ccccgacgtt
    14461 gatcgtcgtc gtggtcttac ccactccgcc cttttggttg atgatggcta tgcgccgcat
    14521 cggacctcca ttgccgagat gctgatggat agcctccctg ccgcctgcaa ttcagcaagc
    14581 ggatctgggc tttccaggat tcgtcattct atctgaatat gaggagccga tgcaaagcat
    14641 tgacggttcg acgcccctgg tcgtgtggtt tttcgagatg cgatcgccgt ggagaatcag
    14701 gtgggggaac aggattggcc cccgatcagg gtcagccgat agcgatcgcc cagcttctcg
    14761 acctcacaga gacaaccgag cgcttccgaa agaacggggg ttgtgagggt ctcgttcttg
    14821 ggtcctgctt tcaacacagt accgttcttg agtaccagaa cgtggccgat ccaggggccg
    14881 atctcttcaa catggtgtgt gaccatgatg atggtcggtg cgtgcggcga tcgtgccaag
    14941 cgttccaagt cggcgaggag gctctctctg gcggccgggt cgagtcctgc acacggttca
    15001 tcgagcagca agagttgcgg ccgattgacc agagccctgg cgatcacaat ccggcgttgc
    15061 tctccttgtg aaagcaggcc gaaggggcgg tgcatgatct ggtcgccgcc gacatgtcga
    15121 atgatttctg ttgccgtaag ccactcggcc tcggagggct gccggtaaag gccgattgac
    15181 gcaaagaagc ccgacgccac gatgtcgatc gcgcgttcat agacgggtaa ccggctttca
    15241 atcgcactgc tggcccatcc cacctgcttg cggaattcag gcaaattgca cttcccaaat
    15301 gattgacgaa gaaccgtcac agaccctgtt gtgggccatt catatcccgt gatgaccttg
    15361 agcagggtgg tttttccgga accgttggcg cccaacaagg cccaatgctg gcccttcttg
    15421 acatgccagg tgatatccgc cagaatccgc tgcccttgac gaaccatcgt gatgtttgat
    15481 aggtggataa ggctcatttt gtgaagagct gcaatctgga tagaggctaa gaatcgccta
    15541 tcttggccga ttttgacccc ctttcgccct tgccgcttgt gagttgggcg gtaatgtaca
    15601 gcgtctttgg cgggcgagtc aaaggaagga tgcctgcggt ttttttgggg catggggggt
    15661 tgcattgaat cggattgtag gatatggttt cggtcgtccg atagaatgaa ggatgtccat
    15721 ataggcagac ggaatacggt cgaaaggcct gaggcgcggt tggttctgcc tgtgagcaat
    15781 gtgcggggtc cagcttttca tgctggaccc cttgtcaagc taactcatgg acactatgct
    15841 atgcgaccga tgtttatagg tctgccgtat tcccaggtca tcacctgggt gccatggcgc
    15901 gaggtagttt tgaacaactc ggcgcagtga cgccgatgcc tggacgtgag tggaagtttt
    15961 cctctgtttg tttttttggc ggcctgaggt tttgtgctag ccagtatctt caaaggagaa
    16021 gaacgatgaa gaaagtcatg tttgcgattg tgtcggcggc aactctgttt gcgtgtggct
    16081 gctgcatgcc gaagatcggg tgctgcaccg cgggtgtcct ggtggacctc cttgagctgt
    16141 tgctgggcgc gatgcctgcg gcctaatcct tgtgttgtga tcggagtgac cgaaagggtc
    16201 acgagggccc ggattggatc gcgacggatg ttctgaggaa actgattgcg gggatccggg
    16261 gcatgggtgg gaatcgcaag gctaggacgt gaggcgggag taacggacgt cacggtggat
    16321 gagcgccgat aacctgacgt gagtggaagt gtttccctgt ttgttggttt tggcggcctg
    16381 aggttctgag ctcgccagta tcttctaaag gagaagaacg atgaagaaag tcatgtttgc
    16441 gatcgtgtcg gcggcaaccc tgtttggttg tggctgcggc tgcatgccca agatcagttg
    16501 ctgcaccgcg ggggtcctgg tgaaccttct cgagctgctc atgggcgcga tgccggcggc
    16561 ctaatccctg taatcagggt aaccgacaag ggttgcagaa cccgagtcgg aatacgacac
    16621 ggcaagagat ttggggcgaa tcagagaagt gctggttcgc cccatctgct tttggcggtt
    16681 gatcgcgccc cgtccttgcc ggtggtacgt ctgccccact gcgtgctctg aacgcaggca
    16741 ggggtgatcc ggtacgtttc ctggggactc aaagcatatc tttactgcag aatctgcgtg
    16801 ccgatcccct tgtcggtaaa gatctccagg agcagtgagt gagggatgcg gccgtcgatg
    16861 atatgagcct tccgggttcc ggcctccagg gcccgcaggc aagcctgaac cttcgggacc
    16921 attcctttgt cgatgatgcc gcggttcatg aaatcggtga tttccttccg gttgagcgtc
    16981 gggcggaagg actcgggatc tttgggattc tcgcggatgc cgtgagtgtc cgagaccatc
    17041 accagttttt cggccttgac ggccgcggct acctcgccgg ctgccgtgtc ggcattgcag
    17101 ttcagcttgc ctccgctgcg gtcacgggca atcggggcga tgacgggaat cgtgtcggcc
    17161 tcacagagca gctcgagaat cctggcgttg actctctcga tctttccgac gtagccgaca
    17221 tcgatccggc ggttctcctc cccacataga tacatctgct cggcgaacaa gacacagctt
    17281 gtcagactgt tcagcccgat agccgcaaca ccgagcgatt ccagcgtctc gacgagccgt
    17341 cggttgatgc ggccgcagag aacctgctcg gcgatagcca gcgttcggtc gtccgtatag
    17401 cgaagtcctt ggacaaaaac cggctcaagg cccgcgtcgt ccatcgccgc attgatctct
    17461 ttaccgccgc catgcacgat gatcggctgc attccgacgt agttcatgaa cactacgtct
    17521 cgcagcagtt ccctttcggt ctcggggtta tccatgaccg aaccgcccag tttcaccacc
    17581 acgatcttgt cgtaatatcc ttggatatat ggcagagctt cgatcaggga tcctgccttg
    17641 gcgatggctt cctgcacggc ttgggttcct ttcactgaaa acgaaacgca aacgttacga
    17701 atcaccgccg ggcaagtcaa gatccggcaa ggccgccgcc gttatgtggg cccgtgacag
    17761 aagcggcggc acttctggcg gctgagccgg ttgtcgtgaa cgcaagccga aagcagagcg
    17821 cttcagcggt ctttcccgaa gggcaaatag gccggtcttt tacgactggt tcacggtcgg
    17881 ccaattcctc cttcgctgtg ttaccccatc gggggccgtt ttaacgggcc gagaccctgg
    17941 aaaggaaagg gaagaaccgt gggcgaccac caaccggccg tataacggcc ggcctgtcag
    18001 cccgcctttg gcgggaacac cccttgaaga ggttgatcgc ccaacctgga ccggtcggca
    18061 tgcccgtcca gacggtcgcg cacccccggt atgccgccgt cgccacgttc cgcttgtcag
    18121 ggggcattca ccgctcttcg ttgacagccc gcccaggcac aattaatgtg acagccaatg
    18181 gcttctcgat gcggtcccaa atcaggcaca tggacggagc ggctcgccat gattgggatg
    18241 accagaggat gacgcgggca atcggcaccc gcggcgcagg gtgtcgtgct caaagcgtgg
    18301 gtatccacgg cttttcttga acgggcggct gcggcattga cggccggtcg ccgaggtttg
    18361 ttttgtctat tggccgcgcg gcgcgaggcg ggtcgcagtg agtaattggt tcgatgccgt
    18421 ccttgtggga ctgttcatgg tcagtacggc catgttgtcc gtctacggtt tgcaccttta
    18481 tatactgctg ttcctttttc atcgccgccg cagcgtcaag ggtgccgagc aacgagcatt
    18541 cgtagaccac tacctcgcca acacaccgga gcgcgagtgg ccgggcgtca ccacgcagat
    18601 ccccatctac aacgaggccg atgttgccac tcgggtcatg gaagcggtgg cggcgatgga
    18661 ctatcctcgt ggcaagcatt cgatacaggt tctggatgac agcacggacg aaacccgcct
    18721 gctggtcgat cgcaccgctc gcaggttgcg ggaacagggt gtcgatatcg aagttcttca
    18781 ccgcgacaac cggcagggat tcaaagccgg ggccttgaaa gccggtctgg ctcattgtcg
    18841 tcatccgctt gtggcgattt ttgatgctga cttcgtgccc ccgaaggatt tccttcgtcg
    18901 cgcggtgccg ttgctggccc gagatgagaa ggtggcgtgc gtccaaggcc gctgggcgca
    18961 cctgaaccag gaagaatcct ggctgacgaa agcccagtcg gtggccttgg acgcgcattt
    19021 tgcagtcgag cagggggcgc gagcttggaa cggcctgatg atgaacttca acggcacggc
    19081 cggcatctgg cgcaaggcgg ccattgagga tccggccgtc ggaggctggt ctgccgacac
    19141 cttgacggag gatctcgacc tgtcttaccg tgtccagctc gccggctggc gaatcgacta
    19201 ctgtctggaa ctggcctgtc ccgcggaatt gccgagcacg atcagcgccc tgaaggcgca
    19261 gcagcggcgg tgggccacag ggggtgtcca gacggcgatc aagctgctgc ccagaatctg
    19321 gcggtcatcc ctgggcctgt cgcacaagat cgaggccacg ctacatttga ctcattacgc
    19381 ggtctatctc tggaccctga tgctggcgct gctggcccgg cccatgctcg cgatggttga
    19441 tccgcttccg ttctggtggt gctttgtgtt cggctgggga ttgatgattc tcacggttgt
    19501 ggcctcgccg atcgtttacg gctatgcccg ctaccatctg ggcggcggcc tcggcggcgt
    19561 gcgaatgacg ccggtgatgc tgcttcttgg caccggtttg tcgatcaaca acgccctggc
    19621 ggtcatccgc ggcctgtacg tacgaggggg cgagttcgtg cgcacgccga aatccggcag
    19681 cacccggaat gccaggttca ccagtcgcta taaggcccag aggttccgcc ggctgtggtt
    19741 cgcggagatg ttgatcggtg catactgttc ggccacttgg gcgatctatg tcaccgccgt
    19801 tcacgggccg gcattcagcg tttttctggc catctatgcc gtttcgtttc taaccatcgg
    19861 ttggatctcc cgcccggaag ccggcctctc gtcgagaccg cgcccgctgc cggaggcgga
    19921 ggcagttgcc ggaccggctg tccctgcgag gctggccccc gccaattcgg tgcatgtcgg
    19981 ttgacggtgc tcgggcggca ggccctctcg tctcgattgt ctgcagggcc tttcacgatg
    20041 cttgtccggc aatggttcag ccctgcagtc ggctgcggtt ttcgtatacc ttgcggaacg
    20101 cggcgacaat atcgtccatg tcctcgcgcg tgcccagcag cattcggtgt tcaagccacc
    20161 cgccctgttc gtagcagatc ttctcgcagt tcgggcaaca ggtttttgcg tagtcgatgc
    20221 cgcggcagcc gtcgaagggg ccgaacgcgc ggtccaagaa cacccgctgc ttataaaggg
    20281 gcagcgggta gcctccgaag tcagggattc cttcggccgc aagcgcctca agaaatgcct
    20341 gcctgggagc cccgagttcc tgggcgtcga tgcggaagca gtacaagtga aacgcgtggc
    20401 gcgtgcactc cgggatgatc ttctgcggct tgattccggg gatctggccg agctgttcgg
    20461 tgaggtaccg gccgttctcc catcggcgtc gggcctgttc ctccagccgt tggagctgtg
    20521 cgttgagcaa agcaccctgg atctcgctca gccggaagtt cgccgcaatc acgaaatgct
    20581 cgtaccacgc atggccctcg cgccgcccgc agttatgaat ttcgcgacag gcctcggcca
    20641 gcgcggggtc gttggtggtg atgaaaccgc cttcaccggc ggtgaggttc ttgctggatt
    20701 ggaaagagaa tgttcccatg tgaccgatgg acccggcgcg gcgccccttg tactcggccg
    20761 cgtgggcgtg ggcggcgtct tcgatgacca ccaggttgtg gcgcctggca atggccatga
    20821 tcgcatccat gtcggccggc agaccggcga agtggaccgg aataatggcc ctggtgcgcg
    20881 aggtgatcag ggcctcgatc gccttggggt cgatattgaa cgtgtccagc tcgatatcgg
    20941 cgaagaccgg cgtggcattg gaggtcacga ctgcggtggc cgtcgccaag aaggtgtagg
    21001 gcgggacaat gacttcatcg cccggtttga taccggcggc catgagggcg acttggatcg
    21061 cgactgtacc gttgaggacg gcgatgccgt gccgggcacc ctggtatcgc gcgaaatccc
    21121 gctcgaatgc ggcgacctcg accccgtcga gcttgcccca cttgccgctg cgcagggctc
    21181 gcaggacggc ctgttcctcg ggttgtccgt agaccggcca ggaagggaac ggctttgttc
    21241 tgaccggtgt gccaccgtga atcgcgagtt gggctgccat cggcctcatc ctttcgttga
    21301 tgtaccggcg ttgctcggtg ccgggcgtgc tacgtactcg atctcggcag gccggaagcg
    21361 tccggcgttg gatcatcggc cgggccggac tccaggaccc ttgccaacag ccagccgccg
    21421 agcacgacgg cagcggccag gccgccgatc atcacgggcc agtcctttac gaataccgca
    21481 ttggttgcca gaaccagccc caggcacgcg aggataccga gaatcgagtc aaagaccaac
    21541 cgagcggagg aggggtcgtg gcgacccgcg aggtcggtgt caccgcgaac cggtccccag
    21601 aagcctggag gccggcatcg tttatagaac cgtttcagcg tttccggcga ctcgggcggc
    21661 gtcagcagcg tcacgattgt cagcagcacg aagctgagca cgaacagcat gccgagtccc
    21721 tgccacatcg gtttttcctg atagcccagc acaaaccaga ccaggatctc gataggcaac
    21781 cccagcagga tcgccgccaa ctcaccccac acgttgaacc gccaccagaa gaaccggaaa
    21841 catgccaggg gcaggaagaa gataaccatc gcgttgttga tgaacttgaa ccaactcacc
    21901 atgtcgtcga cgaacaaaag ggtgatcgtt gccgcgagga caaacagcag gagtgtggtt
    21961 atccgactga cccatacctg gcgacgggga tcgttttgcg aaggcagcag gtcgttgacc
    22021 acaaagctgc cgccccagtt ggtcaacgag ctgagagttg acatgtaagc cgccgcttcc
    22081 gccgcgacaa gcaggcccac aaaaccgaca ggcaggttgc accgcttgat gagttccccc
    22141 caggcatggg ccggcttgtc gagcgaggac atgcccgccg gcgctggtcc cagttgtgca
    22201 tcggaggcag acgtccagaa cagggccatg gcgatgattc ccagtccgat caatggcagc
    22261 gtgcgcagcg tgagagcaag gaaacagttg aacaactgtc cgccgatcgc gtggcgttcg
    22321 tttcgggccg acatgaaccg ttgtgcggtc atcccttcgc cggcggtcgg cgagccggcg
    22381 aagaaccatc cctgcaagac gaggatcagg acggtcagag tgtcgatgct tctggtcggc
    22441 ggcacctggg tcagagcctc cggacggacg gcggtgatgc gagccaggat ctgttcccat
    22501 ccgccatgct gcggtaccgc caaccagaaa aacagggtgc tgccgagcag catgatcgcg
    22561 aattgaacca cgtccgagta caccacgccg agcagaccgc cgaacatcgt atagagcacg
    22621 gtggtcccgc cgaagatcaa cagtatcctg ctcgtgttca acgcgcacag cggctcgata
    22681 gtctgtgcga agaaccccgt gatatagctg atgatcagca cggagaaccc gaaacagcag
    22741 acgaacgcgt atatcttgcg ggcgatcatg gccggtttgc cgccgtagcg cagagaaatc
    22801 agctcggcgg ttgtgacgat ccgcatgcgt ctccagaatc gggcccagac gatggccacc
    22861 agcggcatcc agatgatcca acttgcccac cagatccagt ttccggccaa cccgaagacc
    22921 aggacgatca tgacgaaggc gccgttgccg gactggtagg tagccgtgtt ggccaggccg
    22981 atcgcccacc agggcacgtc gcggttgccg gtaaagtagc cctcggctcc tttttttgat
    23041 gagcgtcgcg agaaccatat gccgaccacg aagaccagtg cacacgaggc cgccacaacc
    23101 agccagtccg ccttgctcat tctgttacct cacgctgacg ggtcgcaagt tgcttgcccc
    23161 acctctttcg gcggatacga gttcgtcagc gagcaatcgt agcgcacgcc tattgctcga
    23221 ttcggtactc tgcccggact tccggactct ccttgtaccc gagccggaca gccttggctc
    23281 tgacggtggc ggtgtgactc agccggatcg gctcgcagta cagcctccaa tgagcttgct
    23341 cgccctcttc acaggtccag gcgatggatg ccgcctcggt cggacagaag atcgtgatcg
    23401 aggcaggccc ccgatggctg ccaccgggag gttccatcac cggtgcggcg gtcacggccc
    23461 atctgccgcc gggtcgcatg cgctcgttca gttcctcttc cggaatcaga ccaaggtccc
    23521 tggtgtcgtc catccatttc tccaggacct tgcgcatttc gatcagtctc tcgcggtgct
    23581 cgggcagatt caccaggttc gtgatctcgt gcgggtcatt gatgcagtca taaagctctt
    23641 cctctggttt ttcgggcagg aagaaggttt tccggggtcc ggtcagcgta ccttccttgt
    23701 tgagccggcg catctcctgc atggtcggca tcttttccat gtagtcgatg tactgggcgt
    23761 aaggacgccc gggcttgaag ttgcggatgt acttgaaccg cttgtctcga acggaccgga
    23821 tgatgtcata ggtctcgtcc atgcggtcgc gtgcggcgaa aacgtattgg cgaggcccgg
    23881 ccctctgccc gccgaggaac ggctgcccct gcatgtgagc gggcaccttc acccccgcca
    23941 gcgagagcac ggtgggcccg aaatcgatga aactgatcag gtcatcgcac accgtgccgg
    24001 catccttgcg gtccggcagg cgaatcacca gcggaacgtg tattcccgag tcgtagagcc
    24061 accgcttggc cctcggcagg ccgcgcccgt tgtcgccgaa gaagaagacg atggttttgt
    24121 ctgccaggcc gtcttcctcc agttgtctga gcaggtcggc caactgaaga tccatggcgg
    24181 tgatcagatc gtagtagcgg gcccagtcgt tgcgcacgat cggggtgtcg ggatagtagg
    24241 ggggcagaac cgctttggca gggtcatggc gatgctcggg ggcgaccttc tgcatgtgcc
    24301 gggcgaattg ctcgggcgag cagcgaatct ggctctcgtg ggtcacggtc agattgaaca
    24361 cggagaaaaa cggctgcccc ggcgtcctgt tgcgccaatg ggccttgttg ctgcattcat
    24421 cccacgcggt cacgggggcg tcgaagttgt agtcggtctt ggagttgttg gtgcagtagt
    24481 agccggcgga ccggaggtat tcggtgaagc acttcacgca ggccggcggc acggccttgc
    24541 atcgcatgtg gtgcgtgctg atggtggtag ggtacatgcc cgtgatgatg gccgaacgac
    24601 tgggggcgca aacgccggaa accgagaacg catgcgtgta gcgaatgcct tcacgggcca
    24661 gtttgtcgat gttcggcgtc cgggcgtact tgtcgccgta acagcccaaa tcggggcaga
    24721 cgtcctcaca ggagatccac aagacgttcg gaaggtcggc ggcattggcg gtttgggttg
    24781 ccgcgatcag gccaagcaac aggaataccg ccgaactcag ttgtctcatc gcgtctttcc
    24841 tgtctttgag ttcatgcatg gccacgcaaa gccgtggcca tgacacccga gttccaaggc
    24901 ggccttcggc cgcaaccaaa gagggcttgg taagccccta tagcaaagac ggcgttctta
    24961 cctgaccgtg gtaaggttta tgaaaccatg gaaaggagaa cgccgtggaa cgattcatcg
    25021 acaagcatca ggcccagatc actggcacga tttcctgctt cgaccggatc ctgttcaagg
    25081 gccatctgcc cctgggccgg cccgaggcca tggaacaatt catagcccgg cagggcactt
    25141 tgatcaagga cttcggagcg ttcgtcaccc gccagtccga gaaggtcaaa cggcacgccc
    25201 aagccacggc ccggcgagcc ggtcggccgt tcatccatcc aaggcggacc gtctttttgc
    25261 ggaccgcgag ccaagcacac tgcgactcag aaacattcgc gcgagccgca caggcgtccg
    25321 gccgcgaggt tctcacgagc cgaggggctt gtcccctcga ttttctgggg cgaaccaagg
    25381 ccgcgctcaa gaatgacttg aacgaattcg acggcgcggc aggattgagt cgttctggtc
    25441 gccgtgcaaa cgatcatgag ccggcaggcc agttcgtcat cgatcacctt gagggcagta
    25501 tcatacctct gagtgtcggg ggctgcacga acgatcggtc ctgtccgttt ggtggcgccg
    25561 gcaatgaggt tgacgacggc ccgtcggctc gccagaggtc gccctcgccg ggggtccggc
    25621 gcggcgatcc cgaatgtccc aatccgaatg gagagcatcc cggcaggaat gacaggaata
    25681 gactctagaa tccacctata ggcgcatgcc cgggcaaagc cgcgggcatg gcagccggcg
    25741 agtttgcgtg tacaatgctc cgaccaagct ccgaaagcac gccgtcaggc gggaaaggac
    25801 aatgatgtat caggcaggca taacgacctt gcggaagatc atcgttcggg cagcattcat
    25861 cgggctgttc gtatcatcgc tgccggcggt tgctcaggct cccagctcag ctcaggaagc
    25921 cccgccgcgt atgccggccg cggtgcagca tgcgccttca gtcatgccgg atcgcatcgt
    25981 gttgacctgg acaggcgaca ccaccacgac gcaggcggtc acctggcgga cctcaacgga
    26041 ggtcagtctc gcctactgcg aggtcgccga ggcggaggcc gggccgcagt tcccccagcg
    26101 ggcccggcga gtcaacgccg tcacccgggt gttcaggtgc gacctgtcga agtgtcacat
    26161 gcaccgcgtc gaaatgaccg atctcaaacc tgcaaccaga tacgtgtacc gtgtcggcga
    26221 cggtgagaac tggagcgagt ggttccagtt ccgcacggcc tccgcggccc cggagcggtt
    26281 ctcattcatt tacttgggcg acggacagaa cgacctgcac agcatgtggt cgcgagtcgt
    26341 tcggcaggcg tactcggacg cgcctcgtgc ggccttcacg ctacatgcag gggatctggt
    26401 caccagcgcg gagagcgacg ccttgtgggg cgagtggttc gccgccgggg cgtggatcaa
    26461 cgccacggtc ccggtcatcg ccacgcccgg caaccacgat tacgcctcga ccaagaacca
    26521 ggacggaacc agccgtcgct ggttgtcccg gcactgggcc gtccagttcg cgttcccggc
    26581 caatgggccg gagggacttg ccgagtcggt ctactacatc gactaccaga actgccgcat
    26641 catttcgctc aactccaatg agaaacaaga ggaacaggtc ggctggatgg acaaggtgct
    26701 ttccgagaac aagaagacct ggacggtgat cacgttccac catcccatct tttcgacggc
    26761 caagggccgg gacagcactc cgctgcgcga gctgtggaaa cccgtgttcg atcgtcatgg
    26821 cgtggacctg gtgctgaccg gacacgatca cacctatggg cgcagcggat tggtggcgcc
    26881 cgagacaaac gtaaccacgg ggtccggtga gcgctcaatt tcgggcggca cggtgtacgt
    26941 ggtgtcggtg agcggcccga agatgtacga tctcagtccc gcatcgcgcg ccgaggtccg
    27001 ccgctcggcc gaggataccc agctttacca gatcgtctcg atcgacggcc ccgtgttgcg
    27061 ttacgaggcc cgcacggcca ccggcgatct gtacgacgcg ttcaccttga agaaacgagc
    27121 cggccagctc aatgagatga tcgagcaggt gcccaacgtg cctgagcgcc gccgtcccgc
    27181 agaaaccgca aagcaaagga gaagaaatgg ggccggttga cctgtacgcc gacggtacgt
    27241 tcgacaacaa gtacgtcacc tatggatgga aaccccagga gtaaccgcgg ccccgcgcac
    27301 gatgagaccc gtgatcggcc gttcttttca gcacgggcgt ctggcggtga tcagaaacac
    27361 cgggaactcg tgttcgctgc cgtcctgcac gggcctgcga atggtgtgag cagtggtgtc
    27421 ccgggtctcc tcaaatccga tttcctcaag ccggatcttg agttcgcccc ggtcgaatcc
    27481 gttatgatgc acgctgccgg cggcgtgcgg gggatgaaag atgcccggct cggtgtccag
    27541 atcggcaatg cccagcattc cgcccggcca tagcagttca cagaaagccc tcatcaccgc
    27601 gtccgcatcg gcaacgtgat gaagggtcat gttggtgacg atcaggtgat agcgttcagc
    27661 gggcagggga tcgtgctgta gatcgagctt gatgacgctc atgttgctga cgccgctctc
    27721 ggcgattttc ttgcgaagca cgtttagcat tgcagaggaa ctgtcggccc cggtcacgct
    27781 gcggacgtgc ggcaggatga agaggccaat cagccctgtt ccgcagccgt aatcgagagc
    27841 atccatctct ctggtgggtc gcacttcgcg aagtattgcc tcgccgaccg ccttcatcaa
    27901 ggcgatgcgg cgcggttcgt tgtcccaggt cgcggcggct ttggcaaagt agtcaggttc
    27961 catattcatt cttggtcggc ctcatcgtcc atcggtcgga ttcttgtcca catggaatct
    28021 gtcgtcagca atcgtccgtc ccgttcgctc ctcagtcagc cagccgcttc cagcgtccgg
    28081 gaagctgtcg aacgcgggca cataaggttt gatgtccagc agcggcgtgc cgtcgagtat
    28141 gtccagctcg cagacgtgga gaacgtcttt ttcgatacgc agcagccgga cgcatgacag
    28201 gccgatgggg ttcggccggg ccggggcccg cgtggcgaac agaccacgtt cttgtgtgtc
    28261 gcgataaggg atgacccgca tccttggggc cgccgagcag tggcaccaat aaagaagcca
    28321 gattcgctcg aatccctcga gatccgcaag cccttccgcg aagggcttga acacctcgac
    28381 ggttccctcg gcgtaggcgg catacaccgg ctggatcggc gtgcctgccg gttggataaa
    28441 cggagtgtga atgattccaa tgggtcttgc ttccaaggtc atggtcatac ccgtcccagc
    28501 acgccgcgac ggtgtctacc ggtttttcag agccggtagc cgcatttgtt gttacgggct
    28561 cagtgcgtca gcggacgatg cagccatcaa tcctgtgtcc tgggcggaac gagacaatct
    28621 atacacagca ccatcgttgg cgtgcaagct tccttaccag gccgtgtccg gcgcgaccgc
    28681 ccaggtcgcc ggaggggcga tctctcattc ccctgcgggt ttcatttcgg gcactttctc
    28741 agggtcatga accagtcgag cacccgtgta tccgacgcgg ctttctcgac gcgctccttg
    28801 gcggtgccgc aggatcccgg cgggggcacg ataacgtcgt cgcccggctg ccagttggcc
    28861 ggcgtggcga cgttgtgctt gtccgacgtc tgcatggcga tcagcaatcg tttgatctca
    28921 tccatgttcc gtccgttgct gagcgggtag tacagaatcg ccctgacaat ggcctgcgga
    28981 tcgatgataa acaccgcccg cactgcctgg gtgttgctcg ccgccggctg gagcatgccg
    29041 aacgccctgg agacctccat cgtcaagtcg ctgatgacgg ggaagtcgat ctcgacgcct
    29101 ttcatgccct tgtaatcgat cttttccttg atcgttcgca gccaggcgat gtggctgtag
    29161 gtgctgtcaa tggacagacc caacagcttg cagttgagct tttcgaagtc cggctgcatg
    29221 caggcaaaag tcatgaactc ggtcgtgcag accggcgtga agtccgccgg atgagagaag
    29281 aacacaaccc acttgccctt gaaatcatcg gggaaggtga taggcccctg ggtggtttcc
    29341 gctttgaaag acggggcctt ttcgccgatc agcggaagct tgttctcgca gcacatgaat
    29401 gttgctcctt cctaaggcgg accgcctttt tgcgggccgc aacccaaacg tcttgcgatc
    29461 cagaaacatg cgcgcaagcc gcacagatat ctggccgcaa ggttccaagg attcagtcga
    29521 acaccaaacg cgttccagtc acgcaatgaa agcaccgact tgggagttcc cggtgaagtc
    29581 ttgccatggc cgcaaatccg gtgcagtggc cgaggcctac gtgctcgata ttcaggctcg
    29641 acaaccgcgc ggccgtctca gccatgcgtc gctccccggc ggccaacaga tgcatcccgc
    29701 ccatgatcgc gtggatgcgg gcctcgccgg tggctgcgcg aatgtgctcc agcgtgttca
    29761 cgacgccggc atgcgcgcag cccagcaata cgacgagccc tttggttgtc tcgatgaaca
    29821 ttgcctggtc atcgatgacc aggtctgctg cggtgcaggc ctcgtcgagg tagaatgcgc
    29881 cccccgtgtc ctcatactca ttccggcggg gtatctcacc ggtgagccag atgccgccgc
    29941 ccaagccgac aggtccgccc ctggtggcga tcatctgccc gacgcgggaa ctcaaccagt
    30001 cgcgggattc gacgggagca ccgacgggac gcacctggcc gtcgtgatcc ttgacgaatc
    30061 gcgtgcgaaa ggccgcgagg tgcgcgtaca ccttcgcctg cctgaagttc gacaatgccg
    30121 ccgccagtcc gccggtgtga tcgtagtggc cgtggctcag ggcgatctca tcgacggcgg
    30181 acaaatcgat acccaggacc gccgcgttgt gctgcatcgc caagccctgg cccgtgtcga
    30241 acagaatgcg acggccgtct cgttcgatcc agaaggccag tccgtgctcg gccagcagat
    30301 tcaagcggtt ggcggtattc tcgaccagta cggtgattga cagcatggtc agctcttgga
    30361 gtaccatccc accacagaaa atgggatgag ttttcattgg gtcattggac cggcatcata
    30421 ccggtcccca gatgcgtttc cgggcgcgca gttgccggtc gagcatcagc atttccccct
    30481 gataaaggaa aaagaaactt gcttcttggc cggatcgtaa ccgaccgaaa acaagcctgc
    30541 gagctttctg ccgcttccgc cgacgcatca cgaacaagat ctccgcatgc ggagtcccaa
    30601 aaggcgccct tggccgttgc acacagagct ccatcttgaa tgacctgcga ttccagatag
    30661 taaagcggcg ccttggcgtc cacaagcctc gcgcgaacca cgaccggcac acccaagacc
    30721 acggctcgat gataacggat actgagcttg gccgtcagtc cggccacgtc ttgagcgaaa
    30781 aggcagtgtg tcattgcagc gtcgagcagc atggccacga cgccgccatg gagacgatcg
    30841 ggatatccct ggaaggcggc accgcagtgg aactcggcga cgacgcttcc gtcggtttgc
    30901 tgttggaaat gcagagccaa gccgtccgtc cggttgctgc cgcacaccag acatgacgga
    30961 tgacaccgac ggctcacatc agtggtcgca gacattctca cctgcctcga gcgaccctgc
    31021 cagataggcg gcaacaattt catcggggtc tctgtcgggc gcaccgacga tcacctcgat
    31081 gccgctctgg gcgaacaatt gctgtgcccg ttggcccatg cctccggcga tcacggcgtt
    31141 gaccccttgc tcatgcagcc agcgtggcaa tatgcccggt tcgtggggcg gtggtgtcag
    31201 gtattctctt cggatcgttc tgcgcgtcgc gggatccact tcatggatca caaactgcga
    31261 gcagtgcccg aagtgggggc acaaacggcc attcgcgact ggtacggcaa tcttcatatc
    31321 ttgctcccgt gttcgttgct gccgggcaat tcccggccga ccttgaaagg tttacgccca
    31381 gtggcctttg acatccgcgc cttgtgcccg ctggagtgcc ccggccttga actgttcgat
    31441 agcttggtta accgttcccg atgcgtccgt gtagacctcg atgccggctg ccgagagcgt
    31501 tgagaaggct ttgggcccca catgtccagt aatcaccgct ttggctgcca gatcgacgac
    31561 ccttttgcct gtctgaatgc cggcgccctg caccgcgttc aggttggcca cgttttcaaa
    31621 cgcagtgcac tcgccagtct cggtgtccgc aagaatcagg tattgagccc ggccgaaacg
    31681 cgggtctacg ggcgaatcag ccgtcggccc ctgcgctgtc acgaccacct tcatggaaga
    31741 cctcctcttt ggtttgcgac agggtttaga gcaaggaccg ttccaacgag catcatggca
    31801 atcgattcct tgtaagttat ttgtatagcg agccttgtga tttcggcacg gcgagcgcac
    31861 acggcctcgt ccacttcgaa gcggtcccgc agtattgcag aatgcactat ttggccatgt
    31921 gaggtctgct acggccatcg gtgggcgggc tctcgatttt gagggctttg agcttgcgga
    31981 atagcgtgct ggggttgatt ccaagatcgg cggcagcccg tttgcggttg ccgtgatggc
    32041 gacggagggc ctcggcgatg aggaggcgtt ccattgattt gagcgtcgtt ggggtgccgt
    32101 gttctccgcc cgcttggctg gttaccgggc gaagttccgg tggcaggtgg tgcagttcga
    32161 tcaagccacc ctgacacagg acgaaagcct gctcgatgat attctccaat tcgcgcacgt
    32221 tgcccggata gtcatgctcc atgagccggg ccaacacctc gggtgatact ccggcgatat
    32281 cctttccctg cagtcggctg aacttggcga tgagtcggtc gatcagcaag ggaatatcct
    32341 cccgacgctg cttcagaggt ggcagcttga gatagaccac gcggatgcga tagaagaggt
    32401 cttcgcggaa ctttccggcg cggacgaggc tgagcaaatc cttgttggtc gcagccacga
    32461 cgcgaacgtc caccttgatc ggctcgaccg atcccagggg ttcgatgcga cgttcttgca
    32521 gaacacgaag cagccgtacc tgcatggccg gagagatatc gccgatttcg tcgagaaaga
    32581 cggttccgcc atcggcgagg gcgaaacgcc cgggcttgtc tcggcgggca tcggtgaacg
    32641 ctccggcttt gtgaccgaac aactccgact ccagcagggt atccggcaga gcggcacaat
    32701 tgatggccac gaaacgcttg cgcctgcggc gtgacagatt atgaatcgct cgagcgaaca
    32761 gttctttgcc cgtgccgctt tctccttcga tgagtaccgt gctgtcgctc tcggcgatcc
    32821 gagggaggag ttcaaacaag ctgaccatcg ccggactgcg accgacgatg tcctcaaacg
    32881 tataccgggc ggtcaattgc ttctgtagct gttcaatctg gctgagatcc tggaacgtct
    32941 cgactcctcc gataaccgtt ccatcgggtt ttttcaggat ggcggtggag aggcgaatcg
    33001 gaatgcgctg tcctgactga ttaacgatgt gcgcggtggc attcacgacc ggtttgccgg
    33061 tgctcaaggt gcggcgcaag gcgcaggagc cttcgcagat gctggcgcga aacacttcgc
    33121 agcagggctt ccccatggcg tcctgccggt caacaccggt gatgcgctcg gccgcctgat
    33181 tgaatgacgt gatccgccag tcgagatcaa tggtgaagac gccctcgttg atggaatcga
    33241 ggatcacctc ctgggaccga gcatatgtcg actgcacgtt tacgacctcc gggttctcac
    33301 caccagtgta ggcacacgaa ttgcataacg caatgaaagc ccgacagcaa gtgttgcaat
    33361 aagcaactct atcggaacaa atccagtcgg tcttagtttt tcctaacatt atttttagaa
    33421 atggtttacg tccattacgt tggcataaga gtacttggca tgcggattga gacctgggga
    33481 tagtgatgaa aatcgcgatt cctcagtggc aggatcgggt gtcgccggtg ttcgatgtcg
    33541 ccggcaatct gctggttgtc gacgtggcga acgggcggga agaagctcgc ctgaaggtcg
    33601 acctgaccgc cacggaaccg ctgcggcggg cgcagcaggt agcaggactg gcaccggatg
    33661 tactgatttg cggggccatt tcctggccgc tggaaatggc cctgacctct gcgggggtgc
    33721 gagtcatctc gcatatctgc ggcgggattg acgaggtgct ggaggctttc gcgaacggca
    33781 acctggcaga tgagaggttc attatgcccg gttgttgccg tcgccggcat atgcgaggcc
    33841 gacgatatcg ccacggcggt cttggcggcc cgaggggggt gcggtgagcg cgcctccacg
    33901 atggccaaaa gcaaaagccg atggcaatgc ggtcttcgag gctgggcgtg aagacgcccc
    33961 tcaacgtgaa gttgtgtgat cgaatgtggg tcgccgggga tcgatcggcc aatcagatcc
    34021 atccggcgta agagaaggga ggttgtagat atgccagcag gtgatggaac agggccaatg
    34081 ggaatggggc cgatgacagg ccgggccgcc ggctactgtg ccggatttgg cgtgccgggt
    34141 ttcgcgaatc ccctaccgag aatgggttgg ggacgaggag gcggttgggg acgcggtggc
    34201 cgcggatggc gtcactggtt ctatgcgacg ggtttgccct tctgggcgcg aatgcccgtg
    34261 cccggcgccg tgtccccgat ggccgctgag ccgacgactg agtatcagac gcaggttctg
    34321 aaggctcagg ccgaacagat ggaaaaaacc ctcgagcaaa tccgcaaacg gattgcggat
    34381 ctcgagtcga tgcaatcaga agcccggtag cctgccggat ccacgacagc cggtcagggt
    34441 agctacccgg gcgaaaggag aatgattcac atgtgtccga gctgtgcatt gggactcttg
    34501 cagtggattt tggcgattcc aatggccttc ttcggcctgt ttggaggagg cgttgtatat
    34561 ggaggtttcg ttggggagac ctgatatgcg cacggctctg tggactctgt tctgtagcgg
    34621 cttggtcttg gcggcctacg gacgtccgac aggcgtcttg gatccgtcgg attcgaacac
    34681 gttgcttatt tttcacaacg gcacggggcc gatgtgcatc gaggcgctga attggctggc
    34741 tgggatacag gcggaggaac cggatctggt tgtggagcaa catctgacga cggatccggc
    34801 cagtctcgcc cttttggggc agttgcgatt gcagtatggg cagagccagg gcgtatcgac
    34861 cagcttcgag tatctgccat tcatcttctt ccggggcgag gcgttctccg ggttcagcga
    34921 aggggtgaag cagtcgctga cgtggttgat cgagtcaatg gacgcgtcgg cttcctgaga
    34981 aagccggcgc ttgaggaaga accaaacgga ggctcagcat tgcgcggcaa gcaacatcga
    35041 ggacggagcc gatgcggcat gggcggcaga ggaggcgggt gccgtcgagg gcagacagac
    35101 ggggcgggta tcgagagatc gaggagcgag ccggctgttt cgtcagcggc accgcttggc
    35161 accgcgtctg ctcggcagcc ggagcgaacc gagacgcttg ctgagctccg acagcgggcc
    35221 gagatcctgg agcagcaact ggcagagttg aggtctcgcg atgtcccggg agccccggcc
    35281 gttgtcaggc gggtggccgt ggtcaaccgg gcccggtgtc gggcctgcgg gttgtgtgtt
    35341 tcggcgtgcc caagagaagc cattcggatg gcccgctgtg ccgaagtgga tgcctccctc
    35401 tgctcgggtt gcggcgcctg cgtggatgtg tgtccagttg aagcgatcag tctgcaaccc
    35461 cgtgaatcgt gatgacaggc ggggattctt catgcggaga tgcccggctc aggcgccttg
    35521 gcggaatctg gaggtattgt gcagacctat ctggactgtg tgccttgctt tgtgcggcaa
    35581 gcacttgact cagcccgcca cctgaccggc tcgccggaag tgcatgaacg acttctgcgc
    35641 gaggtgttgg cgctggcgtc aacgatgtcc ttcgacagtc cgccgccggt aatgggtcgt
    35701 ctgatccatc gccgacttcg agaacttacc ggccaggctg atccttacga ggcggccaag
    35761 cggaaggcca acgagtttgc cctgcggctc tatccggacc tcaagcacgt ggtcatcagt
    35821 tcgagagacc cgtttgcggc cgcggtcagg ttcgccattg gcggcaatgt gatcgacttg
    35881 ggcgtcaaga cgggcctgac cgagagtgac atccgctcgg ccatccgcga agcgatcgaa
    35941 gggcaggtgg atccggatgc cgttgcggca ctccggcaga cggccatgcc cgcgggtgac
    36001 atcctatacc tggccgacaa cgccggtgag atcgtctttg atcgtttgtt gatcgagcag
    36061 atgccgcccg gaagcgtgac cgttgtggtc aaggccggtc cgatcatcaa cgatgccacg
    36121 cgggaggatg ccgaagccgc cggcctggcg gatttcgtgg aggtgatcga caacggggac
    36181 gacgcgccgg ggaccatcct ggaccggtgc agcccggcgt ttcgcaaacg gttcgatgcc
    36241 gcggagctga tcatcgccaa ggggcagggc aactatgaaa cgctcaatga gtgcggtcat
    36301 cgcaacctgt tttttctgct caaggtcaaa tgtggcgtca tcgcccgtga tctccagtgc
    36361 cgcctgggcg agatggccgt gcggaaacat ggggttgtct cgcagagccc tcttaagcgg
    36421 cccggtgatg atttcgaccg gctggcggag gaggcacact catgaagatt ggagtggcgg
    36481 cgacgggcgg ttcgccggac gcgggggtgg cggagcagtt cgggcgatgt cgatggttcg
    36541 tggtggttgg tggcgcaggg agtcgattgg aatgctgagg gacattcgca tacagctcaa
    36601 ggctcacgtg cccttcaccg cctttggcac tgccacaggc atcgccatca tggcgctcat
    36661 tgtgtggacc ggtgtgtccc agtccacgtc cagcgctctt ttctggacct ttcatcccat
    36721 tcatgtgttt ctcagcgcgc tggcgacggc ggcgatgtac aggcttcaca gcggaggcaa
    36781 gctgttggca atcgtcctgg tcggatatgt cgggtcaatc ggaatcgcga ccttgagcga
    36841 ttcggtcatc ccgtacgtcg gggagtatct attgggattg ccgaataggg gcgtccactt
    36901 tggatttatc gaaaagtggt ggctggtcaa tcctcttgcg ctgttgggga ttgccgtcgc
    36961 gcgcctgtgg ccacagacga agttcacgca ctcgggccat gtgctgctga gcacctgggc
    37021 ttccttgttc cacatgacca tggctttggg ggacaacgtg accgcactga cgtgggcact
    37081 tcttcccgtt tttctgttcc tggcggtatg ggttccctgc tgcacgagcg acattgtgtt
    37141 tccgctgatc ctggcgaggc gccgattgcc ggtcgacgct ccgcgagaac aaagcgagga
    37201 ggcgtaccga acatcatgag aatcgctata gccagcggca agggcgggac cggcaaaacc
    37261 acggtggcga cgaacctggc ctacgtagcc tcgctcgacg gccgcaccgt tgcctacgtt
    37321 gactgtgacg tggaagagcc caatggacac ttgtttctca aaccggagtg gctggtaagc
    37381 aagccggtca cgcgccccgt gccgcaggtc gatgaaacca ggtgcacgca gtgcggtcgc
    37441 tgcggcgaga tttgccagta cagcgcgatc gtgccgctcg gaaccacggt gttggtttac
    37501 cccgaactct gtcatggctg cggcggatgc aagctggtgt gcccggcggg ggccatcacc
    37561 gagaccccac gccagatcgg cgttctggaa atgggatggt cgggagacat ccagttcaac
    37621 cgcggcgtgc tgaacatcgg cgaagcgatg agcccgccgc tgatccggca ggtcaagtcg
    37681 gcgcccccca agacggatct gacgttgatc gacgccccgc ccggcacctc gtgtccggtg
    37741 atcgcaagcg ttcgcggcgc tgactgcg
//
LOCUS       DAOTPW010000015        36780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1241_length_36780_cov_5.180994, whole genome shotgun sequence.
ACCESSION   DAOTPW010000015 DAOTPW010000000
VERSION     DAOTPW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 36780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36780
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1241_length_36780_cov_5.180994"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="P5316_02390"
     CDS             <1..1001
                     /locus_tag="P5316_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRR83834.1"
                     /translation="IFDFCERVDLSAVNRGVLDALIKCGAFDSTGATRKGLTMVLDDA
                     IAAGSSAAADRRCGQLSLFGGPGGLVPRAEPRIPGEQWSEAEMLAHEKATLGFYVTRH
                     PLTSHEQTLLKYATARTSDLKRYDDGAEVILGGLISRMRTMLTKSGRNAGSKMAILTL
                     EDLWGQVEVMVFSRDLEKYQAQLAPESLVFFKGRVDRRREEPSLKATEVIPMEFGDER
                     LSSSVLLKVNCVGAEASLLKRVRDTLDRFRGDRTVLIEMLTSGNLKVTLRANGRTGVT
                     PTADFRRAMEGVLGEGSVVVKGSGRSLEAQAGFVGGRQEQPDEPAAYVEEADDEPV"
     gene            complement(1016..1918)
                     /locus_tag="P5316_02395"
     CDS             complement(1016..1918)
                     /locus_tag="P5316_02395"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRR83835.1"
                     /translation="MTRRRLLLIGLPSLLSLVTVAQSGERVLYEKKSPYNLVVVTEDD
                     RGLRSLLFERGGARQSVVKVGDPDHIELPYAKVMLVGLGLVKPPKRVLVIGLGGGTIP
                     GILHKHYPNTMIDVVDIDPDVVKVAKEFFGFREDATMRVHVADGRRFVEQCEEPYDMV
                     FLDAYGDEEIPYHLATQQFLEAVRRALNPSGIVIANLWSRGLNPLYDSMVRTYQRVFA
                     SVYLLDVRDTGNRIVVALPQEQRLERRTLARKARAVSRNKRLKFDLGDLVMQGFRQEP
                     EVDPDAPVLKDKVPTTTAASQPVG"
     gene            complement(2061..4274)
                     /locus_tag="P5316_02400"
     CDS             complement(2061..4274)
                     /locus_tag="P5316_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HRR83836.1"
                     /translation="MIAADASTPPSRTVFGHPAGLFTLFFAEMWERFSFYGMRALLVL
                     YMLKGFLEYGDSDAYAVYGSYCALVYMTPFFGGMLADRLLGARRAVVLGGLLMAAGHL
                     MMTVQTGVGFFSALALLICGNGFFKPNISTIVGSLYPPGSPRRDGGFTIFYMGVNLGA
                     AMSPLLCGYIGETIGWHYGFGLATIGMLVGIAVFVAPSLVSQVSMFFLALAAAFNIFV
                     VSDMKLPNEAFRMYPNYVLLAALVLAGWLTFRELGQGRIRESLSSAWLARIVIMAGAI
                     AAAAVLFWQHPDNPFSSAVNVFVGFSLLVAAVVAWVALGRGGLPKEAGAPPDVERLRR
                     PVLGLLSAESAVYLGTLLSVSVFVLLVSGFAVFRVDLRPLTLISDEYTRRLATSEHAL
                     VQILAVFVQEMSRPAGLALLLAGLAAIVYLALEVFRLDRIPRQRMFVVLILTFFQMLF
                     WAFFEQAGSSVNNFTDRNVDRVIEEYRITADQVGRTIEIQPTQEQLGYHNGSQLFTLN
                     ILDQLRKENKDKPDFTITWEVAEDNVGMGVAGRVAEIPTSTFQSVNPIFILLFGLLFT
                     ALWGFLGKRGWEPSTPVKFSLGLLQLGLGFAAFWYGAQTSNERGMVLLAWLYLGYLLQ
                     TTGELCLSPVGLSMITKLSPGHLVSTVMGTWFLATAYSQYLAAIISQFTGVKSDNQAG
                     GMVIPPPTETVHLYGNVFGKIAIAAVISALICFALVPLLKRWMHQEQQSEPTTQA"
     gene            4510..5934
                     /locus_tag="P5316_02405"
     CDS             4510..5934
                     /locus_tag="P5316_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR83837.1"
                     /translation="MPASCRFAAGILALMLMDPFLVPTRAENRGFVRRDGTHFVLNGR
                     RIYVAGTNSYYQMIHRRTGHAGADEVLDEMQARSLTVLRTWAFQDQVEKAGCLQCAPA
                     RRLGPNERPVDFIDPATLVALDQTLAEADLRAIRVVLTLVNNWEDYGGMNRYTLWRFG
                     AVNHDAFYSDATIRTWFKELAALLVNRVNTVNGRTYRDDPTIFAWQLTNEARCSSAAS
                     ATLNAWMGEMSAYIKSLDPNHMVSTGIEGFYGQGHADRNTDSWMSVYGQDFIDNHQHA
                     TIDYATCHIWPQNWGWDPIGSTSAAMTKAERYLQQRMDDARDVLGKPLMMDEFGIPRD
                     NFGTGTSGGPTTVREQFFRDLYYQTCAGSAAGGGAFAGTALWMILDDPTASWDDGNGV
                     FLPQDAALDAIITSHAQYLAGLADPDLNYDGNVDSEDVNIFRACLTGPASGPPVGDCD
                     GADLDHDGDVDQSDFGGLQASLGS"
     gene            complement(6101..8890)
                     /locus_tag="P5316_02410"
     CDS             complement(6101..8890)
                     /locus_tag="P5316_02410"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRR83838.1"
                     /translation="MTSTRVLVVLTVTMLVFPMAAIADLRPTNLRCEYLINPLGIDEA
                     RPRLSWVLESDQRGQKQTACQILVAASEASLAADEGDLWDSGKQVTAETVGIEYAGKP
                     LRAGQRVWWKVRVWDKDERPGPWSEPAWWEMALLSPKDWTGGWICRDEPDATSGEDLY
                     KENPAPLLRKEFTLKAKVRQARAYVSGLGYYELHLNGRKVGDHVLDPGWTAYGKRVLY
                     STYDVTELLREGNNAVGAILGNGWYNPLPLRMWGHINPREHLTIGRPRLILQLNIEYE
                     DGSHQSVVTDPTWKVGDSPILKNSIYLGEVYDARAEQAGWDSPGYDDGKWAAAVQAKG
                     ELGPLQAQTCPPIRATRTVKPVKITEPKPGVFIFDMGQNFAGWVTMTVSAPTGTAVKL
                     RYGELLYPDGTLNVMTSVCGQIKQAGIGGPGAPEVAYQSDTYITKGGSPERYTPRFTF
                     HGFRYVEVTGLPSAPTLDMLEGHALNSDVATAGSFSCSNELFNRIQEMIRWTQLSNMF
                     SVQSDCPHRERFGYGGDILACGETALLNFDMSRFYAKTVRDLQDSVRPNGGFTETAPF
                     VGIADSGFGEGSGPIEWGTAHPLLLWQLYQYYGDKRLMAEQYDAVAKWIELLKSKAVD
                     NIIDNGLGDHETLVPKMIPLTGTAFYYYNVYLMSQIARILGKNTDADDYARLAGQIRD
                     TFNSKFLDARTGVYASGTQCCQSTALFMDLVPEQSRKAAIDALVKDITETHKGHLSTG
                     IFGTRFMLMALSDLGRADVAYTIANQKTFPGWGHMLENGATTLWEHWEFSDNVFSHNH
                     PMFGSVGEWFIKYLAGIRPDPQAVGFDRIILRPCPVADLTWAKGRYDSIRGPIVSDWR
                     IEGDDFHWKVTIPPNTTATVHIPARSPNSVTEGGKPAAKAEGVVFLQMSKGSAVCQIG
                     SGNYSFISKGFWKAD"
     gene            complement(9340..10896)
                     /locus_tag="P5316_02415"
     CDS             complement(9340..10896)
                     /locus_tag="P5316_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRR83839.1"
                     /translation="MSPRQIIRIGAAVLFVGIIVFTPAARADVRLPSVIGDHMVMQRD
                     MPLPVWGWADPGEEVAVTLGDAGISTKAGPDGKWMVKLPVMPAGGPHSLIVKGKNTIT
                     LTDVLVGEVWVCSGQSNMEMGINSIQDAEKEVAAARYPNIRLYHVCPYRTATEPQDDF
                     KAEWHPCSPDTIKKTGAFWTSGFPATAYFFGRQLHKELDVPVGLIATAWGGTRIEPWT
                     PRAGFESVPSLKDIVKVIDEATPKFNAAVRKAVEDTEKWLPAAKKAIEEGKRVPPPPE
                     WPKHRLENNAEPTSIYNAMVHPLVPFGIRGAIWYQGESNHMDGMAYFDKMQALINGWR
                     KIWAQGDFPFYYVQIAPFDAIYPDDTLPRLWEAQLAALSIPNTGMAVTVDIADLKDIH
                     PKNKQDVGKRLALWALAKTYGKTGFEYSGPLYKSMAVEGSKIRLRFDHVGSGLISRDG
                     KPLSWFEIAGEDKKFVNAEAVIDGETVVVSSAEVPQPVAVRFGWSRKAEPNLSNKAGL
                     PASPFRTDKW"
     gene            11210..13390
                     /locus_tag="P5316_02420"
     CDS             11210..13390
                     /locus_tag="P5316_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83840.1"
                     /translation="MGPLRFVPAVVLAACCAASTFAQPLMIPDDFPRFIVPGQDKAMA
                     SLRDLYYRHYQPPAGPLATMWDEWMSAPTLWPAVETGNRSAGLRAAWAKTLSNRHIDA
                     DGYVSTHQHASIAHQHGWPFPFWAQGGPGTWGWHFSLQHVPQGWHGTEEKKQDGWEIV
                     GGKDLGIAEHAWNVELTAPDAAVRTPPLEIDPEQSPFIQLRWRAEDLENAQPYIEWGE
                     GDSGATIPPRRFHFPPITKAQGIVYTMIPVYQSPLWKGKITRLGINFGNTGPAKVGIQ
                     ALFTQYDTRHNINNSCFTRGCCQYFWWTGDLNFLRDNLQRMRLAVRYMMTDFGAAKEK
                     CIVTPFPGHDGRPGFDVAADGKKTTHSGRGIGHNYWDLLPCGYRDGYATVQYYSTLNY
                     MSRLETEISRHPEWNMPGGPLRLDPKELAEHAREVKQYAGQLFWNKDTGRFVTGLDAD
                     GKMHDYGYTFMNCEAIHYDFATPEQARSIMDWLTGKRIVEGDTSKGADIYHWRFGPRA
                     STKRNVEWYMWIWNTPETIPWGGQVQDGGAVLGFSYHDLMARLKVLGPDDAWQRLKEI
                     LAWYDEVQAAGGYREYYKDGTRGTLQGGGPPGGLGMDREFFESILVPQIVVDGFLGLR
                     ARGDGLEINPRLPSDWPEATVTRVALRQLVLDISVKKDRIRISGQGRSPSLLFVYPPP
                     AKWKVTVDDQEGEQVREMYNVVEGPDDRVQLDVADGRTLTLELD"
     gene            complement(13510..15444)
                     /locus_tag="P5316_02425"
     CDS             complement(13510..15444)
                     /locus_tag="P5316_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83841.1"
                     /translation="MNATLNRRIGLGGVLVLLSAVSWGPAATEDLGNGFLHHGVATPV
                     SMHRGTVATVDGQGRNIVLVWLFDHRGGYALLLIDAETGKSQEFPMPFPPGGDCPYAS
                     ILSSKNKFYTHFNSHFVEFDPAKRAFTFHHQTVPQMAMGMTEDDRGVIWSVTYPQSGV
                     ASFDPNTREFKDYGHVHKENWQQYQRYVAADDAGWIYFGLGNTASHIIGFDPRTGKAK
                     PMIPESERGRGTAEVHRDMNGKVYGCPLVGQDDWYEFHNGQARRIGKRPEIRKKPVIT
                     SSQSLFHTAFPDGKRLKSCDLVERTLTVVNPISRETRQVRFDYHSEGAHIMGLAVAPD
                     DTICGGTAFPMRFFSYNPATDKWINRECYIQWNTVARQGDRFFVGGYTHGFLLEWDPA
                     KPWVPTEKGNKASNPLFLTECHPTINRPHELLAHPDGNTLVLAGTPEYGYTGGGLMFW
                     DRKTQTRILLEHTDILPDQATMSLAALPNGKLLGGTTTSPGTGGERKAKQAELYIMDM
                     RTRKLEWHEAVFQGTQDYTDLCPGPEGLIYGVADRARFFVFDPAKRKVVHEQDTRTQF
                     GLTNSQQGPRIFVTDRQGGTYMLFVKGICRIEPGTFEIELLAESPVPVGPGGDYHKGR
                     VYFASGSHLYSYRVSGKSGD"
     gene            complement(15484..18075)
                     /locus_tag="P5316_02430"
     CDS             complement(15484..18075)
                     /locus_tag="P5316_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83842.1"
                     /translation="MRRCSDQGLPMTLIHLSAIVAVASSNLAAETLYNGIVLPDEWPP
                     RIKELDREPMPVPYLRADPEIIPVDVGRQLFVDDFLIEETTLLRTYHTARYHPDNPVV
                     KPDKPWENEPADGKPCAMVFSDGVWYDPAAGFFKMWYMGGYCRSTCYATSKDGIHWDK
                     PSLDVVPGTNVVLEKSRDSNTVWLDLKESNPARRYKMFIVTQRRDAKGGVDMALYYSP
                     DGMHWGEPVAANWAGGDRSTFFYNPFRNKWVYSVRISRDHVGRARLYAECTDLADGLA
                     RIDDIGRLWTCADRLDPRNPNQALKNVEPQLYNLDVVAYESLMLGLFSIWQGDPGRPG
                     EKRNEVLLGFSRDGFHWFRPDRRPFAAVNESPDAWNWGNVQSAGGGCLVVGDHLYFYV
                     SGRGQKRATDAASTGLAILRRDGFASMDAGESPGTLTTRPIRFSGNHLFVNADARGGE
                     LRVEVLDLDGEVYPALSRRFCMPITTDATIQRVRWMEPVDLSALAGKPVRFRFHLTNA
                     KLYSFWVSHDESGASYGYVAAGGPGYTESRDTVGEAAYRASRDVVTSAASRPAPSGEW
                     MCVTSHAAFTPRDTAEDFVLDGKMWLSNGWRSRLGTRDSTFTPDLWSSSDGVKWTLVT
                     DKTPYDPYSEMVVFQDKVWAIKASVWNSTDGVRWTKVLDKTPFGSRGYGEAVVFKDRI
                     WQLGSGADVWSTADGVNWICAVENAPFGTRTASAVTAYDGKLWLMAGNTPGANEPVEK
                     GYKDVTTHNDVWCSEDGAHWTRVLEHAPWSPRQWCVAAVYAGRLWLIGGYDNVRAGNL
                     GDVWTTTDGRDWRPFESPVRFAPRHEVTPYVFDGSLWVVAGNTWPVVNDVWRLTLPAG
                     GPMRDAR"
     gene            complement(18306..18378)
                     /locus_tag="P5316_02435"
     tRNA            complement(18306..18378)
                     /locus_tag="P5316_02435"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18342..18344),aa:Glu,seq:ctc)
     gene            complement(18567..19763)
                     /gene="bioF"
                     /locus_tag="P5316_02440"
     CDS             complement(18567..19763)
                     /gene="bioF"
                     /locus_tag="P5316_02440"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRR83843.1"
                     /translation="MNSNPTPQDWSDLLANLEVQGLRRSFRILESGQGTYINVAGRML
                     MNLASNDYLGLAAHPCIVQAVKEAVDRWGWGAGASRLVSGTMRIHADLESRLATFKGA
                     EAALVCPTGYQANLAAIRGLAGRGDVIFLDKLNHASIIDASLARGPARLEAPVVRVFP
                     HRDYRRLERLLDKAKGTRRRIIVTDSIFSMDGDFADLSMLVELKSRYDALLIVDEAHA
                     TGVWGEHGRGLAEAMDVTEKIDVAVGTLSKALGGLGGFIAASREIIDWLVNTARPFIY
                     TTALPPAACAAAMAALDLVEREPGRRLGLIRMAAWFREQVAGGLGLDIAGSCSQIIPI
                     IVGPVDRTVELSRALEEAGFLVPAIRPPTVPKGKSRLRISLCADHQQTDLARLLDVLG
                     TVCRKR"
     gene            complement(19760..20224)
                     /locus_tag="P5316_02445"
     CDS             complement(19760..20224)
                     /locus_tag="P5316_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR83844.1"
                     /translation="MKRTTGEWQVPLLFGVIHWWQAMTAKKLRQPLDQACGELIATAE
                     QAPPGANDRNLAKWFPLHGDAYLRFITRPGIEPWNNSAEQAMRFVVIDRPITQATRSE
                     RGQGLCERVWTVIAICAQQTRFAFECLREAISAYLPNQPIPSLVPTGPRTGT"
     gene            complement(20548..22341)
                     /locus_tag="P5316_02450"
     CDS             complement(20548..22341)
                     /locus_tag="P5316_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83845.1"
                     /translation="MTDQTNELRRINWSECFAFTNIFKTFRTAIGPTKLLLAMAGLLL
                     MGFSGWFLDAIWSSKHQAVRGAVVEYVVSGRAGAHRDGNREVLAKRLRVLYQEMGGIA
                     DDRVAAVTDRFKDDPNAAVDDAIKDLKSRYAAECAAAKEKVDSLKKQEADREKEYRKQ
                     MAGIARDKNRIAAELEALRPQGVFKTFLSYETECVYQMLDAARVLNFTNLLIPTITSG
                     SGGLKDYGPWADAPASVLNVKDAAFTELSVRTGPHGFGVLPSVFLMLRGLQWLVFKHG
                     FFALLFLLVSLAIWSLFGGAICRVAAMNYARDEQVGFKAAMAFACRKFLGFFAAPLLP
                     VLMVVGIGVCLFVGGLFLSIPYVGEILGGLLMGLALLGGFLIALVVLGAIGGCGLMWP
                     TIAVEGSDAFDAISRSYSYLYSRPWRTAFYALVATVYGAFCYLFVRFFVLVVLKSTQF
                     FVGLGTTWARRPGTGIAEGTKLDAMWARSSFNDLYPLPPTFGAEGWDKAGAVLIGIWA
                     LLLVMLLCAYLVSFFFSGSTIIYYLLRREVDATEMSEIFVEEEPETPATPAATGSTAP
                     ASTPPASGLGETQSADSGGGAGSSTPGEPAG"
     gene            complement(22570..22782)
                     /locus_tag="P5316_02455"
     CDS             complement(22570..22782)
                     /locus_tag="P5316_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HRR83846.1"
                     /translation="MPTLECPICQRSIVYTSIKEVPFRPFCSKRCKLVDLGRWLNEEY
                     RISEEIPPDLTETGSSPPPPADGIAP"
     gene            complement(22846..23169)
                     /locus_tag="P5316_02460"
     CDS             complement(22846..23169)
                     /locus_tag="P5316_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRR83847.1"
                     /translation="MPTYDYECSACGHQFELFQSITAKPQKKCPSCGRAAARRLIGAG
                     AGLIFKGSGFYITDYRSDSYKQAAKSESESSSGKKDSGGETKASSDAKPAAKAKPTNG
                     TGKRT"
     gene            complement(23173..23847)
                     /gene="grpE"
                     /locus_tag="P5316_02465"
     CDS             complement(23173..23847)
                     /gene="grpE"
                     /locus_tag="P5316_02465"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRR83848.1"
                     /translation="MKKKIVIPSEEDVARFGGGQKSTEMPGSPEGAPTSEAATPPAGE
                     TPPESAPSAADQAASPETLNAEAEQWKDKFLRAKADLLNYQRRAEKERAESLRYANAG
                     LVKALLPVLDDLERVVAAAAEHKNDPEALVAGLRLTLENCLKTLKSFGVEPIEAAGKP
                     FDPNQHEAIMQQPSDEHTAPTVLSEMLKGYRLHERVLRPSRVVVSKPAEPPAAAGNSE
                     GKQPEE"
     gene            complement(23854..25005)
                     /gene="dnaJ"
                     /locus_tag="P5316_02470"
     CDS             complement(23854..25005)
                     /gene="dnaJ"
                     /locus_tag="P5316_02470"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRR83849.1"
                     /translation="MAAQRDYYEILGVSRDAGPDEIKKAYRRAALKYHPDRNKNDPDA
                     EAKFKEAAQAYEVLSDPQKRQRYDRYGHEGLRGVGVHDYSHMRVDDIFSMFDDIFGGG
                     IFGGDGRRGGGVDLEMEISLTLEEVAKGVEKTIEFDRQDFCEACSGSGSEPGSPKRNC
                     ATCGGYGQVEQSTGFGMIFGRVVTTCPACRGKGVLISSPCRKCRGSGRQRRHRVLVVR
                     IPAGIGDGQGVRVAGEGELSENGGARGDLHVYVRIKPHPFFERHGQDLLCRVPISFTQ
                     AALGARIEVPSLTGKLEVTIPPGTQHGQMFRLAGKGLRSLRSQRVGDEIVQVWIEVPK
                     RLNKRQEQLLREYAATEDASVLPESKGFFEKVAEFFAGSNREDSGNRDH"
     gene            complement(25187..26794)
                     /gene="groL"
                     /locus_tag="P5316_02475"
     CDS             complement(25187..26794)
                     /gene="groL"
                     /locus_tag="P5316_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246511.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRR83850.1"
                     /translation="MPKQLMFEDAARQQLWQGLSKLSSAVKVTLGPTGRNVVLQKSFG
                     SPRVTKDGVTVSKEIELPDPFENMGAKMANEVAKKTSDVSGDGTTTATILAEAIYREG
                     LKSVTAGENPMALKRGIDAAVKVAVESIKQQAVKVKGDDIAKVGTISANGDAEVGKLL
                     AEALEQVGTEGIIQIEEGKSLATEKEIVEGMQFDKGYISPYFMTNAGTLECVLEDAYI
                     LIHEKKISNLRDLIPLLEKLVSTAKPLLIIAEDVEGEALATLVVNKLRGVLNVCAVKA
                     PGFGDRRKALLGDIAVLTGGQLISEDLGIKIENIEMSQLGRAKKIVVDKDSCTIIEGA
                     GKKADVKSRCDQLRQMIETTTSDYDREKLQERLAKLTGGVAIIRVGGATETEVKERKD
                     LVEDAFHATRAAVQEGVVAGGGVAFVQAIDAVKKARDKADGPEKLGYDIVIRALQAPA
                     RQIAENCGKDGAVVVSEIMEKGTNIGFDGRTGEFVDMFKAGIIDPAKVARTALENAAS
                     VAGLMLTTEVLVTDLKDDQKEVVGAVR"
     gene            complement(26888..27184)
                     /gene="groES"
                     /locus_tag="P5316_02480"
     CDS             complement(26888..27184)
                     /gene="groES"
                     /locus_tag="P5316_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRR83851.1"
                     /translation="MAKLNIKPLDDRIVVEQCEAEDKTAGGIVLPDTAKEKPQRGKVL
                     ATGPGKLLKDGGRGKMSVNVGDEVFYGKYAGSDVEINGKKYVILKESDVLAILE"
     gene            complement(27290..28948)
                     /gene="groL"
                     /locus_tag="P5316_02485"
     CDS             complement(27290..28948)
                     /gene="groL"
                     /locus_tag="P5316_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437359.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRR83852.1"
                     /translation="MAAKQLAFDDEARASLLAGVEKLSRAVKSTLGPRGRNAVLDKGW
                     GSPTVTKDGVTVAEEIDLTNKYENMGAQLVKEAASKTSDAAGDGTTTATVLTEAIYRE
                     GLRNIVAGADPMALCRGIAKAVEAVTEALNKLAKPVKVTAREDLINVAAISANNDRSI
                     GELLAQCFEKVGKDGVITVEEGKSAETNYDLVEGMQFDRGYLSPHFITDQDAMEAVLE
                     KPYILIHEEKISSVTKLVPLLEKIAKAKKPLLIIAEDVEGEALATLVVNKMRGIVEVC
                     AVKAPGYGDRRKAMLQDIAILTGGKCITKDLGIELDAVSIADLGVAKKVRVDNDNTTI
                     IEGAGKTAEIQARCAQIRREIEITTSDYDREKLQERLAKLSGGVAQINVGAATETEMK
                     EKKARIEDALHAVRAAIEEGIVPGGGVALVRCIDALDNVKCNGDEKTGVEVVRKALSA
                     PLKQIAANAGQEPGVVLAKVQAKSGAFGYNAETNAYGDLLKDGVMDPTKVVRAALVNG
                     SSIARLLLSTDCIVAEKPKKDEEKGGPGGMGGGMDDMEGMGDMM"
     gene            29374..29709
                     /locus_tag="P5316_02490"
     CDS             29374..29709
                     /locus_tag="P5316_02490"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HRR83853.1"
                     /translation="MGSYETEPHPLTRLDSSDLDFVLQLVLASGSLKDVAAAYGVSYP
                     TIRGRLDRLIARVRQVLAGKAADVMAETLADLVERGEVTTSAARSILDLHRRAIQATK
                     EHNNERTVV"
     gene            29690..30049
                     /locus_tag="P5316_02495"
     CDS             29690..30049
                     /locus_tag="P5316_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83854.1"
                     /translation="MSEPWFDANTFGTMYGIIGGGVGGTLCGVLGAIGGILAPRGKGR
                     RFVLGSMAFFVVAGAVQLIIGLIALASGQPYGIWYPMVLCGVILVAVMGPLIPVVRNR
                     YAQVDQRRIDAEAIRRS"
     gene            30437..30664
                     /locus_tag="P5316_02500"
     CDS             30437..30664
                     /locus_tag="P5316_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HRR83855.1"
                     /translation="MKNVTQTIYVNGVLKPLEPLPLQEHQRVWLTVQPMNGKSSSSRA
                     EAIERLRSGIARMGFNSGGEPPTRDELHDRS"
     gene            complement(30818..31717)
                     /locus_tag="P5316_02505"
     CDS             complement(30818..31717)
                     /locus_tag="P5316_02505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR83856.1"
                     /translation="MSSDQHRIGWIGTGVMGVSMCGHVMARAASITIHNRTRDKARPL
                     LDRGAVWADTPRQVAEASDVVFTMVGFPRDVREVYMGKDGVLEGARPGTILIDMTTTE
                     PSLAREIDAAARAKGLHAVDAPVSGGDVGAREARLSIMVGGEAEIVQRVRPLLETMGK
                     TVVHQGPAGAGQHAKMCNQIQIAGTMIGMCESLIYARKAGLDVETMLKSISGGAAGCW
                     SLENLAPRICKRNFDPGFFVDHFVKDMGIALEEAKRMNLPLPGLALAHQLYVAVQALG
                     HGRKGTQALMLALEHIAGMPDRE"
     gene            31874..32578
                     /locus_tag="P5316_02510"
     CDS             31874..32578
                     /locus_tag="P5316_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRR83857.1"
                     /translation="MESMDPVIALLTDFGTRDHYVACMKGVILQINPRAIIEDITHEI
                     NPQDIYHGAFVLRQCLPWFPPKTIFVAVVDPTVGTNRRILAARYSDRYVLAPDNGLLT
                     LVHRDAVLQGVRSVENHQYFAAQVSRTFHGRDIFAPVAAHLSKGVAFDHLGPVVDRME
                     VLSLSRPRIHPDGTVDGEVVLIDHFGNLITNIAVTDLTAANIRRHHLHVTVGETVIGP
                     LRDSYADVPPGQPVAL"
     gene            33063..34181
                     /locus_tag="P5316_02515"
     CDS             33063..34181
                     /locus_tag="P5316_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRR83858.1"
                     /translation="MSAQDSWSRRRFLSPRGLGASTGGLIAALVPQIPKRPRSAGKYC
                     QHWCVSRRAMGCEFTVMLPPDQPSPMASAEAALDEIGELEDLLSVYRGDSAVSRLNRQ
                     AAEAPVRVNYRLYELLKQAAQLTWQTDGAFDVATGAMVKAWGFLRGPKRVPSETERRE
                     AFARSGMRHVVFDDEAISVHYAVPGLEINLGSIGKGYGIDQALKRMMADFGTDCVLIQ
                     GGRSSVRAVGSPADDGRGWLLGIQDPLSPDRQLGTVYLKDRALATSSAVNQFFEAQGH
                     RYGHLIDPRTGWPADGLASVTVLAPDAATADALSTALFIMGLDKAAAFCQNHPEIAAL
                     LVPNRNGDDGQDARPRVVTFNLPPEDVRLMPGNDLPGS"
     gene            34238..35335
                     /locus_tag="P5316_02520"
     CDS             34238..35335
                     /locus_tag="P5316_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRR83859.1"
                     /translation="MAQLRPTSQPASQQPPPPTPQLAKPQTVATVRMFTGWTIALLVI
                     LRIFIGWHFFIEGVYKLCQPDWRATSYLVASAGPFREYFRKFVKDVDGREALAINDPG
                     ILEGNELDGPKVAALSKALEESLAARLDEIYDRTVSFYGLDSGQQEEARKIKEGKKAE
                     LDLLCRDKDFIAQIWDYKVLLDEISRQEKVADATVLAFERERLQDMYKRKARIRAALV
                     ARADKIMTGLPTAVYKAKRTATDDKGAKVEKALLTESQLSKGYMKTDFSPSRFSDFSN
                     KWGLTIVGACLILGLFTRLACLGAAGLLAMYYLAMPPLPGVPEGPSEGHYLIVNKNLI
                     ELVTVLMLATTRVGRWGGLDGLLASKAKPAV"
     gene            35425..>36780
                     /locus_tag="P5316_02525"
     CDS             35425..>36780
                     /locus_tag="P5316_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83860.1"
                     /translation="MVLNAQQRQIGRDNFGDASSEVFRITRRQMLSAGLVIPTAAAMY
                     WGYGKLKGAPVKTALIGTGDQGCYAHISQSNPEYVDFVAFSDIRPSSQQRARDAFKAL
                     YGPEKANKIRLEEDYHKLFDDPDIELVIIALPLHLHAPATIAALQKGKHVLCEKLMAK
                     TVTECKQMVNAAAANNRFLAIGHQRHYSYMYANALSLIKQGVVGDIRHIHAYWHRNQT
                     GFDSWSKEIPPEDRKVDIAKYGYESYEQLVRWRIDNRTGGGLMVELGSHQLDAASIFL
                     NHKLPTEMVGTGVTSYFKDGRTIADHIFMQYLFGDQNVFKSLPDRAKEPTTASAPAEP
                     VLLPLPYTTDNTVVTYSSISTNAFDGYGEQVNGSRATLIMDREMEAYLFYEDFKDKSK
                     DTRVDTRIAWAENRIGKPVMSSGGTAAWGSAVGTADTLTSRGYREEQEHLAWLIREIG
                     KP"
BASE COUNT         6990 a        11424 c        11219 g         7147 t
ORIGIN      
        1 cgattttcga cttttgcgag cgggtggacc tgtcggcggt gaatcggggc gtattggatg
       61 cgctgatcaa gtgcggtgca ttcgactcga ccggcgcgac ccgcaagggg ctgaccatgg
      121 ttcttgacga cgccatcgcg gcggggagct cggcagcggc cgaccggcgt tgcggccagt
      181 tatccctgtt cggcggcccc ggtgggctcg tgccgcgagc ggaaccgcgc atcccaggcg
      241 agcagtggag cgaggcggag atgctggccc acgagaaggc cacactgggg ttctatgtga
      301 cccggcaccc gctcacctcg cacgagcaaa cgctgctcaa gtacgccacc gcccgtacga
      361 gcgacctgaa gcgctacgac gacggtgcgg aggtcattct cggcggactg atcagccgga
      421 tgcgcacgat gctgaccaag agcggccgca acgccggttc gaaaatggcc atcctcacgc
      481 tcgaggattt gtggggacag gtggaggtca tggtcttttc cagagacctg gagaagtatc
      541 aggcgcaact ggcgccggaa tccctggtct tcttcaaagg tcgcgtggac cgcaggcggg
      601 aagagccgtc gctgaaggcg acggaagtga ttccgatgga attcggcgac gagcgcctca
      661 gttcgtcggt gcttctgaag gtcaactgcg tcggggctga agcctccctg ctcaagcgcg
      721 ttcgcgacac gctcgatcga ttccggggcg accggacggt gctgatcgaa atgctgacct
      781 ccggtaacct gaaggtgacg ctgcgtgcga acgggcgaac gggggtaacg ccgacagcag
      841 acttccgccg cgcgatggaa ggggtcctcg gggagggatc ggtggtagta aagggctccg
      901 ggcgaagtct ggaagcacag gccggcttcg tcggaggaag acaggagcag ccggacgaac
      961 cggccgccta cgtcgaagaa gccgacgacg agcccgtctg acgatgcggg atccgctatc
     1021 cgaccggctg cgaggccgca gttgtggtcg gaaccttgtc cttgagaacc ggcgcatcgg
     1081 gatccacctc cggttcctgc cggaagccct gcatgacaag gtcccccaga tcgaacttca
     1141 atcgcttgtt tctcgatacc gccctggcct ttctcgccag ggtgcgacgt tcgagtcgct
     1201 gttcctgtgg cagggcgacg acgatcctgt tgcccgtgtc ccgaacatcg agcaaataga
     1261 cgctggcgaa gacgcgctgg taggttctga ccatcgagtc gtagagcgga ttcaagccgc
     1321 ggctccagag gttggcgatc acgatgccgc tggggttcag cgcacggcgt acggcctcga
     1381 gaaactgctg cgtcgccagg tgatagggaa tttcctcgtc gccgtaagca tcaaggaaaa
     1441 ccatgtcgta gggttcctcg cattgttcga caaaccggcg tccgtcggcc acgtgcacgc
     1501 gcatggtggc atcctcgcga aagccgaaga actccttggc caccttgacg acgtccgggt
     1561 cgatatcgac gacgtcgatc atcgtgtttg gataatgctt gtgcaggatg cctggaatcg
     1621 tgccgccgcc caatccgatg accagcacgc gtttgggcgg tttcaccaga ccgagcccca
     1681 cgagcatcac cttcgcgtag ggcagttcga tgtggtccgg atctccgacc ttcacgacac
     1741 tctgccgggc tccaccccgc tcaaacagca ggctgcgcag tccgcggtca tcctcggtga
     1801 ccaccacgag gttgtagggg gacttcttct cgtagagcac cctctcgccc gactgggcga
     1861 cggtgacgag agagagcaga gacggcaagc caatcagcag aagccttcgg cgggtcatcg
     1921 gtcgcaacct ccacacggcg gcccagcgaa tgcaaccatt gtcagcccct ttgccgccgg
     1981 cgacaaggat gcagcttgga tcggcaggtc tccgccgcct tcggcggctc cgagcatgcc
     2041 ccaccttcct gcgcaccccg ctaagcctga gtcgtgggct ccgattgctg ctcctggtgc
     2101 atccagcgtt tgagcagcgg caccagagcg aagcagatga gcgcggaaat gacggccgcg
     2161 atggcgatct ttccgaaaac gttcccgtac aggtgaaccg tctccgtcgg tggcgggatc
     2221 accattccgc ctgcttggtt gtcgctcttg acgcccgtga actgggagat gatcgccgcc
     2281 aggtattgcg agtatgcggt ggccaggaac caggtgccca tgaccgtact gaccagatgg
     2341 ccgggtgaaa gcttggtgat catcgacagg cccacgggcg agagacagag ttcgcccgtg
     2401 gtctgaagga ggtagccgag atacagccac gcaaggagca ccataccgcg ctcgttcgag
     2461 gtctgggcac cgtaccagaa ggcggcaaaa cccaatccga gttgaagcag tccgagcgag
     2521 aacttgaccg gcgtgctcgg ctcccagccc cgcttgccca gaaaacccca cagggcggtg
     2581 aacaggaggc cgaaaagcag aatgaagatg gggttgaccg actgaaacgt gctggtcggg
     2641 atctcggcga cgcgaccggc cacgcccatc cccacattgt cctctgcgac ttcccatgtg
     2701 atggtaaagt cgggcttgtc cttgttctcc ttgcgcagtt gatcgaggat gttcagggtg
     2761 aagagttgac tgccgttgtg gtagccgagc tgttcctggg tgggctggat ttcgattgtc
     2821 cggccgactt ggtcggccgt gatgcggtac tcctcgatga cccggtccac gttgcgatcg
     2881 gtgaagttgt tgacggaact gccggcctgc tcgaagaacg cccagaacag catctggaag
     2941 aaggtcagga tcagcacgac gaacatccgc tgtcggggga tcctgtcgag ccggaagacc
     3001 tcgagggcca gatagacaat ggccgccagg cccgcgagca acagtgccag tccggccggc
     3061 cggctcattt cctgaacgaa gacggcaaga atctgtacga gggcgtgctc gctggtcgca
     3121 agccgcctgg tgtactcgtc ggatatcagc gtcagcggac gcagatcgac tctgaagacg
     3181 gcgaatcccg aaaccagaag cacaaagacg ctcacggaca gcagggtgcc cagatacacg
     3241 gccgattcgg cactgagtaa accgagaacg gggcgacgaa gacgttcaac gtcgggtggt
     3301 gcgccggcct ccttgggcag gccgccgcgt cccagggcca cccaggccac gacggcggcg
     3361 accaggagcg agaatccgac gaacacattg acggcgctcg agaacgggtt gtcggggtgt
     3421 tgccagaaaa gaaccgccgc cgccgcaatg gcccctgcca tgatgacaat tcgtgcgagc
     3481 caggcgctcg agagcgactc gcgaatcctg ccctgcccga gttcgcgaaa cgtcagccag
     3541 ccggccagca caagggcggc aaggaggacg taattcggat acatccgaaa cgcttcgttc
     3601 ggcagcttca tgtccgatac gacaaagatg ttgaatgcgg cggccagggc caggaagaac
     3661 atcgaaacct gcgacacgag tgagggagcc acgaaaaccg cgataccgac aagcattcca
     3721 atagtggcca agccgaagcc gtaatgccag ccgatcgtct cgccgatgta gccgcacaac
     3781 aggggcgaca ttgccgcccc gagattgaca cccatataga agatggtgaa accgccgtcg
     3841 cgccggggac tacccggtgg gtacagcgag ccgacgatgg ttgagatgtt cggcttgaag
     3901 aagccgttgc cgcagatcag caaagccaat gcgctgaaga agcccacacc ggtctgaacc
     3961 gtcatcatca ggtgcccggc ggccatcaag agcccgccga gaaccacggc gcggcgtgcg
     4021 cccaacagcc gatcggccag cattccccca aagaacggtg tcatgtagac aagcgcgcag
     4081 taggagccgt agacggcgta agcgtcgctg tcgccgtatt cgaggaaccc cttcagcatg
     4141 tacaggacca acagggctcg catcccatag aaggagaacc tttcccacat ttcggcaaag
     4201 aacaacgtga acaatcccgc gggatgtccg aacacggtgc gggacggcgg cgtgctcgca
     4261 tcagcagcta tcattcgcag ggctcctgag aagaaaaggg gccgaccttc aaccctcgat
     4321 ccgcggtctc gtcggccggc cggattctac cggatgcgcg gcgaccgggc aaaaacggtt
     4381 gcctccgggt gaaatcggcc ttccttcagg gtaagatagg agtgtgagaa acaggtgcgg
     4441 ggcggctatg acagccgtgt ccggcggatc aatagtcggc cccgcctcga cggggaagga
     4501 gacaggcgaa tgccggcgag ttgtcgcttc gcggcgggca tcctggcctt aatgctcatg
     4561 gatcccttcc tggttccaac tcgggcggag aaccgcggtt tcgtccgccg tgacggcact
     4621 catttcgtgc tcaacgggcg gcggatctac gtggcgggca cgaacagcta ctaccagatg
     4681 atccatcgcc gcaccggtca tgccggcgct gacgaagtgc ttgacgagat gcaggcgcgc
     4741 tcactgaccg tgctgcgaac gtgggctttt caggaccagg ttgagaaggc aggctgcctg
     4801 cagtgtgcgc ctgcccgacg actcggccca aacgaacggc cggtcgattt catcgatccg
     4861 gcgacgctgg ttgccctcga tcagacgctg gccgaggcgg acttgcgcgc aatccgcgtg
     4921 gtgcttaccc tggtcaacaa ctgggaagac tacggcggga tgaatcgata caccctctgg
     4981 cggttcggag cggtaaacca cgatgctttc tactccgacg cgaccatccg cacctggttc
     5041 aaggaactgg ctgccttgct ggtcaaccgc gtgaacacgg ttaacgggcg gacctatcgc
     5101 gatgacccga ccatcttcgc ctggcagttg accaacgagg cccgttgcag ctccgccgct
     5161 tcggccacgc tgaacgcctg gatgggcgag atgagcgctt atatcaagag cctggatccc
     5221 aaccacatgg tgagcaccgg catcgagggt ttttacgggc aagggcacgc ggaccgcaat
     5281 accgacagtt ggatgtcggt ctacgggcag gattttatcg acaaccacca gcacgcaacc
     5341 atcgattatg cgacctgtca catctggcct cagaactggg gctgggaccc gatcggcagc
     5401 acctctgcgg ctatgacgaa ggctgagcgg tatctccagc agcgcatgga tgatgcccgc
     5461 gatgttttgg gcaaaccgct gatgatggac gaattcggga ttcctcgcga caacttcggc
     5521 acaggaacca gcggcgggcc gacaaccgtg cgggagcagt tcttccgcga cctgtattac
     5581 cagacctgtg ccggctccgc ggcaggcggc ggagcctttg ccggaaccgc gctgtggatg
     5641 atcctcgacg accccacggc ctcgtgggat gacggcaacg gagtgttcct gcctcaggac
     5701 gccgctctag acgcgatcat cacctcgcat gctcaatacc tggccggttt ggcggacccc
     5761 gatctgaact acgacggcaa tgttgactcg gaggacgtca atatcttccg cgcctgcctg
     5821 acgggaccgg ccagcggtcc gccggtgggc gactgcgacg gcgccgatct cgaccatgac
     5881 ggcgacgtgg atcaatccga cttcggcggt ctccaggcat cacttggatc ctaaatctgc
     5941 cccgctttgt cgcgaaacga acgtggctcg cacgaaggat atggcagaag ccgtcgcaag
     6001 ccgtcaaatg cgctgcggcc gctcgaactg cacggccggg tggcttacgg cacggtgcgg
     6061 cctactcagg aaaccgcgaa cgccgattgt ggaggaggga tcaatctgct ttccaaaatc
     6121 ccttcgagat gaacgagtag ttgcctgagc cgatctggca gacggccgac cccttggaca
     6181 tctggaggaa caccacgcct tcggctttcg cggcgggttt accgccttcg gtcacggagt
     6241 tgggcgagcg ggccgggatg tgcaccgtgg ccgtcgtgtt gggcgggatg gtcaccttcc
     6301 agtggaaatc gtcgccttca atgcgccagt cgctgacgat cgggccgcgg atggagtcgt
     6361 accggccctt ggcccaggtc aggtcagcga caggacacgg ccgcaggatg atcctgtcga
     6421 agccgaccgc ctgggggtcg gggcggatgc ccgccaggta cttgatgaac cactcaccca
     6481 ccgagccgaa catcgggtgg ttgtgcgaga agacgttgtc gctgaactcc cagtgttccc
     6541 acagggttgt cgccccgttt tcgagcatat gaccccagcc ggggaaggtc ttctggttgg
     6601 caatggtata ggccacgtcg gcacggccga ggtcgctcag ggccatcagc atgaaacgcg
     6661 tcccgaagat gcccgtgctg aggtggcctt tatgtgtctc ggtgatgtcc ttgacgagag
     6721 catcgatcgc tgccttgcgg ctctgctcgg gcacgaggtc catgaacagc gcggttgact
     6781 ggcagcactg tgtaccggag gcatagacgc cggtccgggc atcgaggaac ttcgagttga
     6841 acgtatctct gatctgtccg gccagtctcg cgtaatcgtc ggcgtctgta ttcttgccga
     6901 gaatcctcgc gatctgtgac atcaggtaca cgttgtagta gtagaaggcc gtgccggtca
     6961 gggggatcat cttcggcacc agcgtttcgt gatcgccgag gccgttgtcg atgatgttgt
     7021 cgaccgcctt ggacttgagc aactcgatcc atttcgcaac cgcgtcgtac tgctctgcca
     7081 tcagccgctt gtcgccgtag tactggtaca gttgccacag caggagcggg tgagccgtgc
     7141 cccactcgat cgggccgctg ccctcgccga agccggagtc ggcgatgccc acgaacggag
     7201 cggtctcggt gaagccgccg ttgggccgga ccgaatcctg caggtcgcgg acggtcttgg
     7261 cgtagaaacg cgacatatcg aagttgagca gggcggtttc gccgcaggcc aggatgtcgc
     7321 cgccgtagcc gaaccgttcg cggtgcgggc agtcggactg tacgctgaac atgttgctga
     7381 gctgcgtcca gcggatcatc tcctggatgc ggttgaacag ctcgttcgag caggagaatg
     7441 agccggcggt ggcgacgtcg gagttgaggg cgtggccttc gagcatatcg agcgtcgggg
     7501 ccgacggcag gccggtgact tcgacgtatc ggaagccgtg gaaggtgaac cgtggcgtgt
     7561 agcgctccgg gctgccgcct ttggtgatgt aggtgtcgga ctggtacgcc acctccgggg
     7621 cgccggggcc gccgatgccg gcctgcttga tctgaccgca gactgaggtc atcacgttga
     7681 gcgtgccgtc gggatagagc agttcaccgt aacgcagctt gaccgcagtc ccggtcgggg
     7741 cgctgacggt catcgtgacc cagccggcga agttctggcc catgtcgaag atgaacacgc
     7801 cgggcttagg ctcggtgatc ttcaccggct tgaccgtgcg cgtcgcgcgg atcggcgggc
     7861 aggtctgagc ctgcaatggt ccgagctcgc ccttggcctg cacggccgct gcccacttgc
     7921 cgtcgtcgta gccgggcgag tcccacccgg cctgctcggc gcgggcgtcg tagacttcgc
     7981 cgagatagat gctattcttg agaatcgggc tgtcgccaac cttccatgtc gggtcggtga
     8041 cgacggactg gtgcgagccg tcctcgtact cgatgttcag ttgcaggatc agccgaggcc
     8101 ggccgatggt caggtgctcg cgcgggttga tgtgccccca catccgcagc ggcagcgggt
     8161 tgtaccagcc gttaccgagg atggcaccga cggcgttgtt gccctctcgc agcagttcag
     8221 tcacgtcata ggtgctgtac agcactcgct tgccgtaggc cgtccagccg ggatcgagaa
     8281 cgtggtcgcc gaccttgcgg ccgttgaggt gcagctcgta gtagcccaaa ccgctgacat
     8341 acgcccgtgc ctgccgcacc tttgccttga gggtaaactc tttgcggagc aggggggcgg
     8401 ggttctcctt gtacaggtct tcgccgctgg tcgcgtcggg ctcatcgcgg cagatccacc
     8461 cgccggtcca atccttcggc gacagcaggg ccatctccca ccacgccggt tcgctccagg
     8521 ggccggggcg ctcatcctta tcccacacgc ggaccttcca ccagactcgc tggccggccc
     8581 gaagcggctt gcccgcatat tcgatcccga cggtctctgc ggtgacctgc ttgccgctgt
     8641 cccaaaggtc accctcgtca gcggccagac tcgcctcact ggcggcaacc aggatctggc
     8701 aggcggtctg cttctgcccg cgctgatccg actcgagcac ccaactcaac cgcggccggg
     8761 cctcgtcgat gcccagcgga ttgatcaggt actcgcagcg gaggttcgtg ggtctcagat
     8821 cggcgatcgc cgccatgggg aacaccagca tggtcacagt cagtacgaca agaacgcggg
     8881 tcgaggtcat ctgtctcacc tcactgtctc catttgtttg ctggctgcaa aatcccttca
     8941 ctggtcccag aatcctttat ggggtcagtg ccctcagaat actttctggg gtcgctggcc
     9001 ccagaatccg ttctggggcc ttccgcgtgg aggattccca tcctcattct caccgaacgc
     9061 ggcatggatc cgccaggcca gacggccctg ctcggcggca aacgcttcgc gggacaggcc
     9121 tggaacggat tccgggccga gcggaatgtc agttcgtccg catcctatag ggaatcagtg
     9181 attccggcca aggcgggggt gaacaactca aaacgtgccg acgagggcgt cggcaccacc
     9241 agtagggcca acgccctctt tggcccgtat gcatctgatg cagttgcaga tgatccggcg
     9301 gcgcggaccc tgcccggttc acgtcccaga gcggtccggt caccacttgt ccgtacgaaa
     9361 cggcgaggcc gggaggccgg ccttgttgga caggttgggt tcggccttac ggctccagcc
     9421 gaaacgcact gccaccggct ggggaacctc ggcgctgctc accaccaccg tttcgccatc
     9481 gatgaccgcc tcggcgttga cgaacttctt gtcctcgccg gcgatctcga accagctcag
     9541 cggcttgccg tcgcgcgaga tgaggccgct gccgacatgg tcgaacctaa gccggatctt
     9601 gctgccctcg acggccatcg acttgtagag tggaccggaa tactcgaagc cggtcttgcc
     9661 gtaggtcttg gccagggccc acaaggcgag gcgcttgccg acgtcttgct tgttcttcgg
     9721 gtggatgtcc ttgaggtcgg caatgtcaac ggtgacggcc atgccggtgt tggggataga
     9781 taaggccgca agctgggcct cccacaagcg cggcagcgtg tcgtccggat agatagcatc
     9841 gaacggggcg atctgaacgt agtagaacgg gaagtcgcct tgggcccaga tcttccgcca
     9901 tccgttgatc agggcctgca tcttgtcgaa gtaggccatg ccgtccatgt ggtttgactc
     9961 gccctggtac cagatggccc cgcgaatgcc gaacgggacc agcgggtgga ccatcgcgtt
    10021 gtagatgctg gtgggctcgg cgttgttctc gagccgatgc ttgggccact cgggcggcgg
    10081 cggcacgcgc ttgccttcct caatggcctt cttggctgcg ggcagccact tttcggtgtc
    10141 ctccaccgcc ttgcggaccg ccgcgttgaa cttgggtgtc gcctcatcaa tgactttgac
    10201 aatgtccttc agcgacggca ctgactcgaa gcctgcccgc ggcgtccacg gttcgatccg
    10261 cgtgccgccc catgcagtgg cgatcaggcc caccgggacg tcgagttcct tgtgaagctg
    10321 ccgaccgaaa aagtaggcgg tggcgggaaa accggaggtc cagaacgcgc cggttttctt
    10381 gatggtgtca ggcgagcagg ggtgccattc ggccttgaaa tcgtcctgcg gttcggttgc
    10441 agttctgtat gggcagacat gatacagtcg gatgttcgga taccgggccg cggccacctc
    10501 cttttcggcg tcctggatgc tgttgatgcc catttccatg ttcgactggc cggaacatac
    10561 ccacacctcg ccgaccaaaa cgtcggtcaa cgtgatcgtg ttcttgccct taacgatcag
    10621 actgtgcggt ccgccggccg gcatcaccgg gagcttgacc atccatttgc cgtccgggcc
    10681 ggccttggtc gagatgccgg cgtcgccgag agtgacggcg acctcctcgc cgggatcggc
    10741 ccaaccccag accggcagcg gcatatcacg ctgcatgacc atgtggtcgc cgatgacgga
    10801 gggcaaccgc acatcggcac gggctgccgg ggtgaaaaca atgatgccaa caaacagaac
    10861 cgctgcacca attctgatga tttgacgagg actcatggct tctccttgtg attctgcggg
    10921 ttcgttgtcc aagaactaca cggccagagt atatcaagcg tgttgaagcc tcaatggcca
    10981 tggtcgaact gctcgggtca gcaagccgga gcgattctcc tgtggcatcc gggatgaagt
    11041 gcgaccgaga agggattctc ggtcgagcga aagactggcg ggcaagccgt cagtggcacc
    11101 cggcgaccga gaaaggattc tcggtcgagc gcggccgatc gttgatcgtt cgcatctcga
    11161 gccatacact gcctgtgggt tcgtccgggg tcccgttgga gaatgcgtca tgggtccatt
    11221 gcgtttcgtg cctgccgtcg ttttggctgc ctgctgtgcc gcgtccacgt ttgctcagcc
    11281 tttgatgatt cccgatgact tcccgcgatt catcgttccc ggccaggaca aggccatggc
    11341 cagcctgcgg gacctgtact accggcatta tcagccgccg gccgggccgc tggcgacgat
    11401 gtgggacgag tggatgagcg ccccgacgct ctggccggcg gtcgagaccg gaaaccgaag
    11461 cgccggcctg cgggccgcgt gggccaagac gctgtccaac cggcacatcg acgccgacgg
    11521 ctacgtgtct acgcaccagc acgcctcgat cgcccaccaa cacggctggc cattcccctt
    11581 ctgggcccag ggcgggccgg gcacctgggg ctggcacttc tcgctgcagc atgtgccgca
    11641 gggctggcac ggaaccgagg agaaaaagca ggacggctgg gagatcgtcg gcggcaagga
    11701 tctgggcatc gccgagcacg cgtggaatgt ggaactgacc gctccggatg cggccgtgcg
    11761 caccccgccg ctggagatag acccggagca gtcgccgttc attcagcttc ggtggcgagc
    11821 agaggacctg gagaacgcac aaccttacat cgaatgggga gaaggcgact cgggggcgac
    11881 catcccgccg cgccgtttcc acttcccgcc catcaccaag gcccaaggca tcgtttacac
    11941 catgatcccg gtgtaccagt cgccgctgtg gaagggcaag atcacgcggc tgggcatcaa
    12001 ctttggtaac accggacccg ccaaggtggg gatccaggcc ctgttcactc agtacgacac
    12061 gcggcacaac atcaacaact cgtgttttac acgcgggtgc tgccagtact tctggtggac
    12121 cggcgatctg aacttcctcc gcgacaacct gcagcggatg cgcctggccg ttcgctacat
    12181 gatgaccgat ttcggcgcgg ccaaggaaaa atgcatcgtc acgccgtttc ccggtcatga
    12241 cggacggccg ggcttcgacg tagccgcgga cggtaagaag accacccatt ccggacgcgg
    12301 catcggccac aactactggg acctgctgcc ctgcggctat cgcgacgggt acgccacggt
    12361 gcagtattac agcacgctga actacatgag ccgactcgag actgagatct cgcgccatcc
    12421 ggagtggaac atgcccggcg gaccgctgcg gctggacccg aaggaactgg ccgagcacgc
    12481 ccgcgaggtg aagcaatacg ctggccagct cttctggaac aaggacaccg gccgtttcgt
    12541 caccggcttg gacgccgacg gcaagatgca cgactacggc tacaccttca tgaactgcga
    12601 ggcgattcat tacgacttcg ccacgcccga gcaggcccgt tcgatcatgg attggctgac
    12661 cggcaagcgg atcgtcgagg gcgacacctc caagggcgcg gatatctatc actggcgttt
    12721 cggcccgcgg gcctcgacca aacgcaatgt cgagtggtac atgtggatat ggaatacccc
    12781 tgagaccatt ccctggggcg gccaggtgca ggacggcggt gcggtgctgg gcttctcgta
    12841 tcatgacctg atggcccggc tcaaggtgct cggtcctgac gatgcgtggc agcggctgaa
    12901 ggagattctc gcctggtacg atgaggttca ggcggccggc ggctatcgcg agtactacaa
    12961 ggacggcacg cgcggcacgc tgcagggagg aggccctcca ggcggcctgg gtatggatcg
    13021 tgagttcttc gaaagcattc tggtgccgca gatcgtggtg gacggattcc tcggcttgcg
    13081 ggctcgcggc gatgggctgg agatcaatcc tcgcttgccc tccgactggc ccgaagcaac
    13141 ggtcacgcgc gtggctttgc ggcaactggt gctcgacatc tcggtcaaga aggacaggat
    13201 ccgaatcagc gggcaagggc gatcgccgtc gctgctcttc gtttatccgc ctcccgccaa
    13261 gtggaaagtg acggtggatg atcaagaggg cgagcaggtc cgcgagatgt acaacgtggt
    13321 tgagggccct gacgaccgag tgcaattgga cgtagccgac ggccgcacgc tgactctgga
    13381 actcgactga attgctcgca gtgcgcccag ggattggcct ggtcgacctg tgcgaagaag
    13441 gcaatcctgt atccgcagag ctgctcgggc ttcgccgtca acgccgcgtt gttgtgtttc
    13501 gggagagcgt cagtctcccg actttcccga aactctgtag ctgtacagat gcgacccact
    13561 ggcaaagtag acgcgacctt tgtggtagtc tccgccggga ccaacgggca cgggggattc
    13621 ggccagcagc tcgatctcga aagtgcccgg ctcaatgcgg cagattcctt tgacgaacag
    13681 catgtaggta cccccctgac gatcggtcac aaagatccgc ggaccttgtt gggagttcgt
    13741 caggccgaat tgcgtccggg tgtcctgttc gtggaccact ttgcgtttgg ccgggtcgaa
    13801 gacaaagaaa cgagcccggt ccgccacacc gtagatcaga ccctcggggc ccggacacag
    13861 atcggtgtag tcttgggtac cttgaaagac cgcctcgtgc cactccagtt tccgggtgcg
    13921 catgtccatg atgtacagtt cggcctgttt ggcctttcgc tcgccgccgg ttccggggct
    13981 tgtggtcgtc ccgcctagca gcttgccgtt tggcaaggcc gccaggctca tggtcgcctg
    14041 gtcaggtaga atgtccgtat gttcgagcag gatgcgcgtt tgggttttgc ggtcccagaa
    14101 catcagcccg ccgccggtat agccatattc cggggtgccg gcaagcacaa gagtattgcc
    14161 atcggggtga gccagcagtt catgagggcg gttgatcgtg ggatgacact ccgtcaggaa
    14221 tagcggattg gaggccttat tgcctttttc ggtcggcacc cagggtttgg ccggatccca
    14281 ctccagtaga aacccgtgag tgtagccgcc gacaaagaac cgatccccct gccgggcgac
    14341 cgtgttccac tggatatagc attcgcggtt tatccatttg tctgtcgccg ggttatagct
    14401 gaagaaccgc atcgggaaag cggtgccgcc gcagatggtg tcatcaggtg cgacagccag
    14461 gcccatgatg tgcgccccct cgctgtggta gtcgaaccgg acttgtcgcg tctcgcggct
    14521 tatcgggttc actacggtca gcgtacgttc gaccaggtcg caacttttca gccgcttgcc
    14581 atccgggaaa gcagtgtgaa agaggctttg agaagaagtg atgacgggct tcttgcggat
    14641 ctccggccgt tttccgattc ttcgggcctg cccgttgtga aactcgtacc agtcgtcctg
    14701 tccgacaagc ggacagccat agactttgcc gttcatgtca cggtgtactt cggcggttcc
    14761 ccgcccgcgc tcgctttccg ggatcatggg cttggccttc ccggtgcgcg gatcaaaacc
    14821 gatgatgtga ctggcggtgt tgcccagccc gaagtaaatc catccggcat cgtcggcggc
    14881 gacgtagcgc tgatattgct gccagttctc cttgtgtacg tggccatagt ccttgaactc
    14941 gcgggtattc ggatcaaagg aagcgacgcc gctctgcgga taggtgaccg accagatcac
    15001 cccgcgatcg tcctcggtca tgcccatggc catttgcggc acggtttgat gatgaaacgt
    15061 aaaggcccgc ttggcggggt cgaactcgac aaaatgactg ttgaaatgcg tataaaactt
    15121 gttcttgctg gagagaatcg aggcataagg acaatccccg ccgggcggga agggcatggg
    15181 aaactcctgg ctcttgcccg tttcggcatc gatcagcagc aaggcgtatc ccccgcgatg
    15241 atcgaacagc cagaccagga cgatgttgcg tccctgaccg tcgaccgtgg ccaccgtgcc
    15301 ccgatgcatg ctgaccggcg tggccacgcc atgatgcagg aagccgttgc ccagatcttc
    15361 tgtggctgcc ggcccccagg agaccgccga cagcagcacc aagacgccac ccaaaccgat
    15421 tctccggttc agcgttgcat tcatgctttt gtcctttcgc gtggccatca ccacgccgtc
    15481 atatcacctg gcatcacgca ttgggccacc ggccggcaag gttaaccgcc acacgtcatt
    15541 gaccaccggc caggtattcc cggcgacgac ccacaggctg ccgtcaaaaa cgtacggcgt
    15601 aacctcgtgg cggggggcaa accggacagg cgactcgaat ggccgccagt cacggccatc
    15661 ggtggtggtc cacacatccc caagattgcc ggctcggaca ttgtcatatc cgccgatgag
    15721 ccacagtcgg ccggcgtaga ccgccgccac acaccattgc cgtggtgacc agggcgcatg
    15781 ctcgagaacg cgcgtccagt gagcgccgtc ttccgagcac catacgtcat tgtgggtcgt
    15841 gacgtccttg tagccttttt ccaccggctc attggccccc ggggtgttgc cggccatcag
    15901 ccacagtttc ccatcgtatg ccgtgacggc cgaggccgtg cgagtgccga acggagcgtt
    15961 ctccacggcg cagatccagt tcacgccgtc ggcggtggac cagacgtcgg cgccgctgcc
    16021 tagttgccag atgcggtcct tgaacaccac cgcctcaccg tagccgcgac tgccgaacgg
    16081 cgttttgtcc agcaccttgg tccatcgcac gccgtccgtc gagttccaca cgctggcctt
    16141 gatggcccat accttgtcct ggaagacaac catctcgctg tatgggtcgt agggtgtctt
    16201 gtcggtgacc agcgtccatt tcaccccgtc actcgaagac cacaggtcag gagtaaacgt
    16261 cgagtccctg gtaccgagcc tggagcgcca gccattactg agccacatct tgccgtccag
    16321 aacgaaatcc tcggctgtat cgcgcggtgt gaacgcggcg tgcgaagtga cgcacatcca
    16381 ttcgccggac ggcgccggcc ggctggccgc ggaagtcacc acgtcgcggc tcgctctgta
    16441 agcagcctcg ccgaccgtgt cgcgagactc ggtgtagcct ggtccgccgg cggcgacata
    16501 gccgtaactg gcccccgatt cgtcgtggct gacccagaag gagtagagct tggcgttggt
    16561 cagatgaaat cgaaaccgca ctggtttgcc ggccaaggcg gacaagtcga caggttccat
    16621 ccatcgcacc cgctgaatcg tggcgtcggt ggtaatgggc atacagaacc tgcggctcag
    16681 cgccggataa acctcaccgt cgagatcgag tacttcgact cgcagctcgc cgccgcgggc
    16741 atcggcgttg acgaagagat ggttgccgct gaaccggatg gggcgggtag tcaacgtgcc
    16801 ggggctttct ccggcgtcca tcgaggcgaa accgtcgcgg cgcaggatgg ccaagccggt
    16861 ggacgcggcg tcggtcgcgc gtttctgacc ccggccacta acgtagaaat acaggtgatc
    16921 gcccactacc aggcacccgc cgccggccga ctggacgttg ccccagttcc acgcatccgg
    16981 gctctcgttg acagcggcaa atggccggcg gtccggtcga aaccagtgga atccgtcacg
    17041 gctgaagccc agtaacacct cgttacgttt ctcgccgggc cgacccggat ccccctgcca
    17101 tatcgagaac agccccagca tcaggctttc gtatgcgacg acatccagat tgtataactg
    17161 tggttcgaca ttcttgagcg cctggttggg gttgcgagga tcaagtcgat ccgcgcatgt
    17221 ccaaagccgg ccgatgtcgt ctatgcgcgc aagcccgtcg gccaagtccg tgcattcggc
    17281 atacaacctt gccctgccga catggtcccg gctgattctg acgctgtaga cccacttgtt
    17341 gcggaaggga ttgtagaaaa aggtcgagcg gtcgccgccg gcccagttgg ccgcaacggg
    17401 ctctccccag tgcatgccat ccggcgagta gtacagggcc atgtcgacgc cgcccttggc
    17461 atcacgtcgt tgcgtgacaa tgaacatctt gtatcgccgg gcgggattcg actccttcaa
    17521 gtcgagccag acggtattcg aatccctcga tttctccagg accacgtttg tgcccggtac
    17581 gacatccaga gacggcttgt cccagtggat tccatccttg ctcgtggcgt aacaggtgct
    17641 tctgcaatac ccgcccatgt accacatttt gaagaacccg gcggccgggt cgtaccacac
    17701 gccgtcgctg aacaccatcg cgcagggttt gccgtccgcc ggctcgtttt cccacggctt
    17761 gtcgggcttc actaccgggt tgtcagggtg atagcgagca gtgtgatacg tgcgcaggag
    17821 ggtggtctct tcgatcagga agtcgtccac aaagagctgc cggcccacgt cgacggggat
    17881 gatttcgggg tcggccctga ggtatgggac cggcatcggt tcacggtcca attccttgat
    17941 ccgcggcggc cattcatcgg gcaggacaat gccattgtag agcgtttcgg ccgccaagtt
    18001 cgaggaagca accgcaacga tggctgaaag atggatgagc gtcatcggaa gaccttgatc
    18061 ggaacaacgc cgcatgggat cacctcaacg cagggtccga gccattacga ccacggcagc
    18121 attttacgga tgcctgttaa tcagcgatac ggtcgttgtg tcgctggctc gggcgatgtc
    18181 cccgagaaaa ccgatatgcg ccgaaagtcg ttgctgactc gcatgcaaac acgccatccg
    18241 taaaagcgga aggagcggtc acttatgacc gctcctgtca tcagatggcg agttgtgcca
    18301 ccgcataccc ccagggggac tcgaaccccc gtctccagga tgagaacctg atatcctagg
    18361 ccactagacg atgggggcag gccttgtcgg cgataccaac cgcttttgtc atccgtaact
    18421 tagtctaacg gatcgcaaag atcaaccggt gcccgttcgc atgccggcga tctctgaccg
    18481 gtttctgggg tccttcttcc ctcactggcc gagcctgccc ggatctgacc ctggacatcg
    18541 ccccgccaaa aaccaatcgc gcggactcat ctctttcggc agactgtgcc cagaacatcg
    18601 agcagtctgg caaggtcggt ttgctggtgg tcagcgcata aactgatccg caagcgggac
    18661 ttgcctttgg gaacggtcgg tggccggatg gctggaacca ggaagccggc ttcctccagc
    18721 gctcgcgata gctcgaccgt gcgatcgacc ggaccaacaa tgatcgggat gatctggctg
    18781 cacgaccccg caatatccaa cccgaggccg cctgcaactt gttcgcgaaa ccacgcagcc
    18841 atcctgatca ggcccaaacg tcgccccggc tcccgctcga caagatcgag tgcggccatc
    18901 gcagcagcgc aggccgctgg tggcaaagcc gttgtgtaga tgaagggacg agcggtgttg
    18961 accagccagt cgatgatttc tcgcgatgca gcaatgaaac cccccaggcc gccgagtgcc
    19021 ttgctgagcg tccccacggc aacatcgatc ttttcagtca catccatggc ctctgccagc
    19081 ccccggccgt gctcgcccca tacaccagtg gcgtgtgcct cgtccacgat caggagggca
    19141 tcatagcggc tcttgagttc aactagcatt gaaaggtccg cgaagtctcc gtccatactg
    19201 aagatcgaat cggtaacaat gatccttcga cgcgtccctt ttgccttgtc cagcagcctc
    19261 tccaggcgcc ggtaatcacg atgcgggaaa accctgacaa cgggagcctc caatctggcc
    19321 ggtccacgcg caagtgaagc gtcaatgatg ctcgcatgat tcaatttgtc cagaaatatc
    19381 acatcacctc gtcctgccaa cccgcggatg gctgcaagat tggcttggta gccggttggg
    19441 cagacgaggg cagcctcggc acccttgaag gttgccaggc gcgattcaag atcggcgtgt
    19501 attctcatcg tgcctgaaac cagtctggag gcgcctgctc cccagcccca gcgatcgaca
    19561 gcttccttga ccgcttggac tatgcagggg tgggcagcca gcccgaggta atcgttgctg
    19621 gccagattca tgagcatgcg accggccacg ttgatgtagg tcccctgccc ggattcgagg
    19681 attctgaagg accgccgaag gccctggact tccagatttg ccagcagatc cgaccagtcc
    19741 tgtggggttg ggttcgaatt catgtacccg ttctcggccc ggtcggaaca agggagggga
    19801 tgggttggtt tggcaggtag gccgagatgg cttcgcgaag gcactcgaac gcgaagcggg
    19861 tttgttgggc acagatggcg atgacggtcc aaactcgctc gcacaagcct tggccccgct
    19921 cgcttcgggt tgcctgggtt atgggtcggt caatgaccac gaatcgcatc gcctgttcgg
    19981 cagagttgtt ccacggctcg atccccggcc tcgtgatgaa gcgaaggtag gcatccccat
    20041 gtagcggaaa ccacttggcc aggttccggt cattcgcacc cggcggggct tgctcggccg
    20101 tcgcgatcaa ttcgccgcaa gcctgatcca agggctgccg caacttcttc gccgtcattg
    20161 cttgccacca gtgaatcacg ccgaacaaca gagggacttg ccattccccg gtggtccgtt
    20221 tcatccacgt tcctgcgcgg cacggctgga cccgtgcccg caaggcctcg tactacaccc
    20281 ccgcgaatgc ggcaccgggc ttccagtgtg ccacgtcgcc ctccaacttg gcaatccgtc
    20341 gccgacagat ctgctcctgg gtcggttcca ccgcccaccg ccggcgtctc gttttatact
    20401 tctcgggcaa aaccactccc aaagctcaga agcattaaca cgccatgcca agccccttgg
    20461 cgccagagga taccgtgaac cgttaccgat tccgaaacag ccggcccaaa cggtcacgag
    20521 ccctgtgctc gcccgggttc aacccgatca gcccgcaggc tccccaggag tcgaggaacc
    20581 tgcgcctccc cccgagtcag cgctttgagt ttccccgaga ccggaggcag gaggcgttga
    20641 ggccggagca gtcgagccgg tggcggcagg cgttgcgggt gtttccggtt cctcctccac
    20701 aaagatttcg ctcatctccg tcgcatcgac ctcgcggcgc aggagatagt agatgatggt
    20761 tgagccggag aagaagaacg acaccaagta agcacacagt aacatcacca gcaggagagc
    20821 ccagatgccg atcagcaccg caccagcctt gtcccagccc tcggctccga aggtcggtgg
    20881 cagaggataa aggtcgttga aagaagacct ggcccacatc gcatccagtt tggttccctc
    20941 ggcaatcccc gtgccgggtc ggcgggccca tgttgtaccc aatcccacga agaactgagt
    21001 gctcttgaga acgaccagta caaagaacct cacaaacagg tagcagaacg cgccgtagac
    21061 ggttgccacg agggcataga atgccgtccg ccagggccgc gaatacagat aactgtagct
    21121 tcgggagatt gcgtcgaagg cgtccgagcc ctcgaccgcg atggtcggcc acatcagccc
    21181 gcacccgcca atggctccga gcacgaccaa agcgatgagg aagccgccca aaagagccag
    21241 tcccatcagg agaccgccga ggatttcgcc gacgtacggt atggagagaa acagcccacc
    21301 gacaaaaaga cacacaccga tgcccaccac catcaacacc ggtagcaacg gggcggcaaa
    21361 gaaacccagg aatttgcggc aggcaaaggc catcgcggcc ttgaagccga cctgttcgtc
    21421 ccgggcataa ttcatcgccg ccacccggca gatcgcccct ccgaacaggg accagattgc
    21481 cagcgagacc agaaggaaca acagggcaaa gaacccatgc ttgaagacca gccactgcaa
    21541 gccacgaagc atcaggaaaa cactcggaag gacgccaaat ccgtggggcc cggttctgac
    21601 actgagctct gtgaacgcgg cgtctttgac gttcagtacg ctcgcagggg catccgccca
    21661 agggccgtaa tccttcaatc cgcccgagcc ggaggtgatc gtcggaatga gcaggttggt
    21721 gaagttgagg acgcgggcgg catcgagcat ctggtaaaca cattcggtct cataggagag
    21781 aaacgttttg aagactccct gcggccgaag cgcttccagt tcggcggcaa tgcgattctt
    21841 gtcgcgggcg ataccggcca tctgcttccg gtattccttc tcacgatccg cctcttgctt
    21901 cttgagggaa tcaactttct cttttgcggc ggcgcactcg gcagcgtacc tgctcttgag
    21961 atccttgatc gcgtcgtcga cggctgcgtt cggatcgtct ttgaatctgt cggtcaccgc
    22021 agcgactcgg tcgtcggcga tgccacccat ctcctggtag agaacgcgca gcctcttggc
    22081 caacacctct cgattgccgt ctcggtgggc tccggcccga ccactgacca cgtattcaac
    22141 gaccgcgccc ctgacggcct ggtgttttga actccagatc gcatcaagaa accatcctga
    22201 gaagcccatc aacaacaggc cggccatcgc caacaacagt ttggtgggac ctattgccgt
    22261 ccgaaaggtc ttgaagatgt tcgtgaaagc gaaacactcc gaccagttga ttctgcgaag
    22321 ctcgttggtt tgatcggtca tgactcaacc tccagaatcg aaatgagaga ttgttgcgtc
    22381 ttatcgactg acggtccgac gtagcaccca cttgtgccgg gcatcgggtc ttggccgatc
    22441 gttccggcat cctcctggca tcgatccaac gggtggcatt ataaccgccg acggaacccc
    22501 atcaataccg acgcccaaac gcgtcatcga cgacccgggg tcatcgtcag gcgaaaagac
    22561 ttgaggccct catggcgcga tgccatcggc gggcggaggc ggactactgc cggtttccgt
    22621 caggtcggga ggaatctcct cgctgatgcg gtattcctcg ttcagccagc ggccaagatc
    22681 aacgagcttg cagcgtttgc tgcaaaaggg tctgaagggc acctccttga tagaggtgta
    22741 gacgatggat cgctggcata ttgggcattc cagcgtcggc ataacagcct ttccgcaagg
    22801 cagcaagaag gtcacgacgg gccggcatcc tgcccaacta cgttcttatg ttctcttgcc
    22861 ggtgccgttg gtcggtttag ccttcgccgc cggcttggca tccgaggatg ccttggtctc
    22921 cccgccactg tctttcttgc cgctggagga ctcggattca gacttggccg cctgtttgta
    22981 gctgtcactg cggtagtcag tgatgtagaa acccgaaccc ttgaagatca ggccggctcc
    23041 tgccccgatg agacgtctgg cggcagcacg tccacatgac gggcacttct tctgcggctt
    23101 ggcagtgatg ctctgaaaca actcaaactg atgtccgcaa gcagaacatt cgtaatcata
    23161 agtcggcatg gtttactcct cgggttgctt tccctcacta ttgccggcgg ctgcgggagg
    23221 ctcggcgggc ttggatacga ccacgcgaga cggcctcagg acccgttcat ggaggcggta
    23281 gcccttgagc atctcgctca gcacggtcgg cgcggtatgc tcatcgctgg gctgctgcat
    23341 gatcgcctcg tgctggttcg ggtcgaaggg tttgcccgcc gcttcgatcg gttcaacgcc
    23401 gaagctcttg agagtcttga ggcagttttc aagcgtcagc ctgagacctg caaccaatgc
    23461 ttcgggatca ttcttgtgtt cagccgccgc ggcgaccacc cgctccagat cgtccaagac
    23521 cggcaacaac gctttaacca gcccggcgtt tgcgtatcgc agtgattcgg ctcgctcctt
    23581 ttccgcccgt cgctgatagt tcagcaaatc ggccttggct cgcaggaact tgtccttcca
    23641 ctgttcggcc tctgcgttca gagtctcggg ggaggcggct tggtcagcag ccgatggtgc
    23701 cgactcaggc ggcgtttccc cggccggtgg ggtggcggct tcgctcgttg gcgcaccttc
    23761 cggcgagccc ggcatttcgg tcgatttttg cccaccgccg aagcgagcca catcctcctc
    23821 tgacggtatg acgatcttct tcttcatcgc cgcttagtga tccctgtttc ctgagtcttc
    23881 ccgattcgac ccggcgaaga actcagccac cttctcgaag aaaccctttg actcgggcag
    23941 gaccgaagcg tcctccgtgg cggcgtactc gcgcagcaat tgctcctgcc gcttgttgag
    24001 tctcttgggc acctcgatcc atacctggac gatctcgtca ccgacgcgct gggaacgaag
    24061 cgacctgagc cctttgccgg cgagccggaa catctgcccg tgctgcgtgc ccgggggaat
    24121 ggtcacctcc agcttgccgg tgagagacgg tacctcgatc cgggctccaa gcgccgcctg
    24181 ggtgaagctg atcggcacgc gacagagcag gtcttgcccg tgccgctcga agaaggggtg
    24241 cggtttgatc cgcacataga cgtgcagatc gccgcgagct ccgccgttct cgctcagttc
    24301 gccctcgccc gccacgcgga cgccctgtcc gtcgccgata cccgccggaa tgcgaaccac
    24361 caatacgcga tgcctgcgct gcctgccgct gccgcggcat tttctgcacg gcgaggagat
    24421 cagcacaccc ttgccgcgac acgccggaca agtcgtgacc acacggccga agatcatccc
    24481 gaaaccggtg ctttgctcga cctggccgta gcctccgcaa gtggcacagt tgcgtttcgg
    24541 cgagcccggc tcgctgccgc tgccggagca ggcctcgcag aaatcctgcc ggtcgaactc
    24601 gatcgttttc tcgacgcctt tggccacttc ctcgagcgtc agcgatattt ccatctcgag
    24661 gtcgacaccg ccgccgcgcc ggccgtcgcc accaaagatt ccgcccccga agatgtcgtc
    24721 gaacatcgag aagatgtcgt cgacacgcat gtggctgtag tcgtggacgc cgacgccgcg
    24781 aagcccctcg tggccgtagc gatcatatcg ttgccgcttc tggggatcgg agagcacctc
    24841 atatgcctgg gccgcctctt tgaacttggc ctcggcatca ggatcgttct tgttccggtc
    24901 agggtgatac ttgagggcgg cccggcggta cgccttcttg atctcgtccg gcccggcatc
    24961 ccgggaaacc ccgaggattt cgtaataatc tcgctgtgcc gccactacca tactcgcagc
    25021 aaactcgccg attgagaaga ctcaggtacc gcaccgcagc gatccccgcc ttcgccagcc
    25081 cgtcatgccg ccagcccgca gcgtcgaaga gcgggtttgt cccggctcca tcggcgatac
    25141 gggctggagc cccctgtcag acaagggccg gcatcgactc tatgaactat cggacagccc
    25201 cgacgacctc cttctgatca tccttgagat cggtgaccag aacctcagtg gtgagcatga
    25261 gcccggccac gctggcggcg ttctcaagcg ccgtgcgcgc gaccttggcc ggatcgatta
    25321 taccggcctt gaacatgtcc acaaattcgc cggtccgccc gtcaaacccg atgttcgtgc
    25381 ctttctccat gatttccgag accaccacgg cgccgtcctt gccgcagttt tcagcgatct
    25441 gcctggccgg ggcctggagt gcccggatca caatgtcata gccgagcttt tcggggccgt
    25501 cggctttgtc acgggccttc ttgaccgcgt cgatagcctg gacgaaagcc acgccgccgc
    25561 cggcgacgac gccctcctgg acggccgccc gtgtggcgtg gaacgcatcc tcgaccagat
    25621 ccttgcgctc cttgacctcg gtctccgtgg ccccgccgac gcggatgatg gccacgcccc
    25681 cggtgagctt ggccaggcgc tcttgcagct tctcgcgatc gtaatcactg gtggtggttt
    25741 cgatcatctg gcgaagctga tcgcatcggc tcttgacgtc cgctttcttc ccggcaccct
    25801 cgataatggt gcacgaatcc ttgtcgacga caattttctt ggcccgcccg agctgagaca
    25861 tctcgatgtt ctcgatcttg atgcccagat cctcgctgat caactggccg ccggtcagaa
    25921 ccgcgatatc gccaagcaga gccttgcgac ggtcgccgaa gccaggggcc ttcacggcgc
    25981 agacgttcaa caccccgcga agcttgttaa ccaccagggt ggccagggcc tcgccctcga
    26041 cgtcctcggc aatgatcagc agcggctttg ccgtgctgac gagcttctcg agcagcggga
    26101 tgaggtcccg aaggttcgaa atctttttct cgtggataag gatgtaggcg tcttcaagca
    26161 cgcactcgag cgtgccggcg ttcgtcatga agtaaggcga gatgtaacct ttatcgaact
    26221 gcatgccttc gacgatttcc ttctcggtcg ccaggctctt gccctcttcg atctggatga
    26281 tgccttcagt gccgacctgt tcgagggctt cggcgagcag tttgccgacc tcggcgtcgc
    26341 cgttggcact gatggtgccg accttggcta tgtcgtcgcc tttgaccttg accgcctgct
    26401 gcttgatgct ctcgacggcc actttcacgg cggcgtcgat accccgcttg agggccatcg
    26461 gattctcgcc ggcagtgacg ctcttgaggc cctcgcgata gatggcctcg gccaggatcg
    26521 tagcggtggt ggtgccgtcg ccgctgacgt cgctggtctt tttggccacc tcgttggcca
    26581 tcttggcgcc catgttttcg aagggatcag gcaattcgat ctccttgctg accgtcacgc
    26641 cgtccttggt gactcgcgga gacccgaacg atttctgcaa caccacgttt cggccggtcg
    26701 gccccagcgt caccttgacc gccgacgaca gctttgacaa cccctgccag agttgctgtc
    26761 tcgccgcatc ttcaaacatc aattgcttgg gcatctctat ctgctcctcg cttttcgcga
    26821 ttcctgctct cttgtttcgg gccgcgccgt cgttcgatgc ggccgcttcc gccggttaca
    26881 agggcggcta ctccaggatt gccagaacgt cgctctcctt gagaatcacg tatttcttcc
    26941 cgttgatttc cacgtcggac ccggcgtact tgccgtagaa gacctcgtcg ccgacgttta
    27001 cgctcatctt gccgcgtccg ccgtccttga gcagcttgcc cggccccgtt gccagcacct
    27061 tgccgcgctg gggcttttcc ttggccgtgt ccggcaggac aatgccgccg gccgtcttgt
    27121 cctcggcctc acactgctcg accacgatcc gatcatccaa aggcttgatg ttcagcttcg
    27181 ccatcgcgtc tatctccgtt cgtcttcagg ctccgtatat gaggacccgt ttgttgccga
    27241 aaaccgtaac accactcgct gcaaaagggc gaccgcgtcg tccgcacgat cacatcatgt
    27301 cccccatgcc ctccatgtcg tccattccgc cgcccatgcc gccggggccg cctttttcct
    27361 catccttctt gggcttctcg gcgacgatac agtcggtgct caacagcagt cgtgcgatac
    27421 tgctgccatt gacgagcgcc gcccggacga ccttcgtcgg gtccatgact ccgtccttga
    27481 gcaggtcgcc gtaggcgttc gtttcagcgt tgtagccgaa agcgccgctc ttggcctgaa
    27541 ccttggcgag taccacgccc ggctcctggc cggcgttggc cgcgatctgc ttgagcgggg
    27601 cggacagggc tttgcgcacc acctcgacgc cggttttctc gtcaccgttg cacttcacgt
    27661 tgtccagagc gtcaatacaa cgaaccaagg ccacgccgcc gcccggcacg ataccttcct
    27721 cgatggccgc gcgaaccgcg tggagggcat cctcgatacg agctttcttc tccttcatct
    27781 ccgtttcagt ggccgctccg acgttgatct gagccacgcc cccggagagc ttggccaacc
    27841 gctcctgcag tttctcgcgg tcgtagtcgc tcgtggtgat ctcaatctcg cggcgaatct
    27901 gggcgcagcg ggcctgaatc tcggccgtct tgccggcgcc ctcaatgatc gtcgtattat
    27961 cgttgtccac ccgcaccttc ttggcgacac caaggtcggc aattgacact gcatcaagtt
    28021 caatgcccag atccttggtg atgcatttgc cgcccgtgag aatggcgatg tcctggagca
    28081 tggccttgcg gcgatcgccg tatccgggag ccttcaccgc acagacttcc acgatgcctc
    28141 gcatcttgtt aaccaccagc gtggccaagg cttcaccctc gacgtcctct gcgataatca
    28201 gcagcggctt cttggccttg gcgatcttct cgagcaatgg aaccagcttc gtgacgctcg
    28261 agatcttctc ctcgtggatc aggatgtagg gcttttccag aacggcttcc attgcgtcct
    28321 ggtcggtgat gaagtgcggg ctaaggtagc cacggtcaaa ctgcatgcct tcgacgaggt
    28381 catagttcgt ttccgcgctc ttgccttctt cgacggtgat cacgccgtcc ttgccgactt
    28441 tctcgaagca ctgggcgagc aattctccga tgctgcggtc attgttggct gagatcgcgg
    28501 cgacgttgat caggtcctcg cgggccgtga ccttgaccgg cttggccagt ttgttgaggg
    28561 cctcggtaac cgcctcgacg gccttggcga ttcctcggca gagggccata gggtcggccc
    28621 cggccacgat gtttcgcaag ccttcgcgat aaatggcctc ggtcagtacg gtcgcggtgg
    28681 tggtgccgtc gccggcggcg tcactggtct tgctcgccgc ctccttgacc aactgggcac
    28741 ccatgttttc gtacttgttg gtcaggtcga tctcctcggc aaccgtcacg ccatctttgg
    28801 tgaccgtcgg cgagccccaa cctttgtcga gcacggcatt ccggccgcgg ggccccaacg
    28861 tgctcttgac cgcccgtgac agcttttcca cgccggccaa caagcttgct cgggcctcgt
    28921 catcaaatgc caattgtttc gcagccatgt ccttggttct ccttggtgtt cacttgcccg
    28981 gcccaacccg gccggggcgt tcgtacctac gtcgacaggg attctcccct tggctggcag
    29041 tcagggcaaa cagggtgcca caagaggcat gcggcacgaa gtgttacgct taactctttt
    29101 gaaaatttga aattacgaca atctgaatcg tcagatctga gcccctcgcc gccgtgccag
    29161 cgtggcagca atcccgtccg gtatgccata gtgacagggc cgcgaacacg tccagtaaat
    29221 cacaatagcg gcggtctttg ccaccggaag ccagatcctg atctttaatt ttatgaaaac
    29281 cgatttgctt tttattaaaa tcgaccatac agttgatgca tgtggccaac ggccatgcca
    29341 gcaaggataa gaccaataac ttgaggcaga gtcatgggat cgtatgaaac cgagccacat
    29401 cccctgacga gattagatag tagcgacctc gactttgtcc ttcagttggt tctggcctcc
    29461 ggttctttga aggacgtggc ggccgcctac ggcgtcagtt atccgacaat cagggggcgg
    29521 ctggaccggt tgattgcccg cgtcaggcaa gtgctggcag gcaaggcggc ggacgtcatg
    29581 gccgaaacgc tggcggatct cgttgaacgg ggggaagtaa ccacgtcagc agcaaggtcc
    29641 atactcgatt tgcacagacg tgcaatccag gcaactaagg agcacaacaa tgagcgaacc
    29701 gtggtttgat gctaacacgt tcggcacgat gtatggcatc atcggcgggg gcgtgggggg
    29761 aacactgtgc ggcgttctcg gggcgatagg cggaatcctg gccccgcgag gcaagggacg
    29821 acgattcgtc cttgggtcca tggccttctt cgtcgtggca ggcgcggtcc aactgatcat
    29881 cggcctgatc gccctggcgt ccgggcagcc gtacggcatc tggtatccga tggttctttg
    29941 cggggttatt ttggttgccg tgatgggacc gttgattccc gtcgtccgta accgctacgc
    30001 gcaagtcgac caacgtcgta tcgacgccga agcgatcagg cgaagctaaa tgaaccccgc
    30061 gtgcttctgc ctgagtaagc cattctaagc ggcccgcctc gagagccggc cgctttgtag
    30121 cgcatccgga acaaccgagg ggctccagcg caatccgaca atgctagacg ctgccgttgt
    30181 gaagccgcga caggcctgca gttcaacgcc ggcgaggtct cggagatcac cctgccaatc
    30241 ggcgacctga aggactggga actgaagcac tccgagtggc accgcgcggg cacgacccag
    30301 ccggccacgg cccccgccgc aggtgaggct tcagccggcc ggtgaagtga caccgcaagt
    30361 tgccgatcac ggggacccgc aagtatgatg gagtgtggcc cgatagaagg accaccgacg
    30421 caaccacggt gccgttgtga agaacgttac tcaaaccata tacgtgaacg gggtactcaa
    30481 gccccttgaa ccgttgcccc tgcaggagca ccagcgggta tggctgactg tccagcccat
    30541 gaacggcaag tcatcgtcat cacgtgccga ggcgatcgaa cgtctgcgca gcggcatcgc
    30601 tcgaatgggc ttcaactccg gcggcgagcc gccaactcgt gacgaactgc atgatcgctc
    30661 ttgacacaaa cgtccttctt gtactctgga aacatacctg cccaaagagt gccggaattg
    30721 gagatcatca accccttcgt acagtagcct tcgggaacgt tcaaagacgg caggtctcag
    30781 tcgcccgagg cgacttcgac cgtgccctac ctcgcgctca ttcgcgatcc ggcattccgg
    30841 cgatgtgctc gagagccagc atcagggctt gggtgccctt gcggccgtgg ccgagggctt
    30901 gcaccgccac gtagagctga tgagcgagtg ccaggccggg cagaggcaga ttcatgcgct
    30961 tggcttcctc cagcgcgatg cccatatctt tgacgaagtg atcgacgaag aagcccggat
    31021 cgaagttgcg cttgcagatg cgcggagcga ggttttcgag cgaccagcat ccggccgctc
    31081 ctccgctgat gctcttgagc atcgtctcga cgtcgagccc ggccttgcgg gcgtagatca
    31141 ggctctcgca catgccgatc atagtgccgg cgatctggat ctggttgcac atcttggcgt
    31201 gctgaccggc gccggccggt ccctggtgga cgacggtctt gcccatcgtc tccagcagcg
    31261 gccggacgcg ctgcacgatt tccgcctccc cgcctaccat gattgacagc cgggcctcac
    31321 gcgccccgac gtcgccgcct gagaccgggg catccacggc atgcaggccc ttggcccttg
    31381 cggctgcgtc gatctcgcgg gccagtgaag gctctgtcgt ggtcatgtcg atgagaattg
    31441 ttcctggccg tgcgccttcg agaacaccgt ccttgcccat gtagacttct cgcacgtcgc
    31501 ggggaaagcc caccatggtg aacaccacgt ccgacgcctc ggcgacctgc cggggcgtgt
    31561 cggcccacac tgcaccgcgg tcgagcagtg gccttgcctt gtcccgcgta cggttgtgga
    31621 ttgtgataga ggccgcgcgc gccatgacgt gaccgcacat cgacacgccc atcacgccgg
    31681 tgccaatcca accgattcga tgctggtcgg aagacatgtg atgatctcct gagactacgt
    31741 gaccgcgaga gtcttgacca ccttgacgag ccggcggctt tatgccgccg gtcttcacgg
    31801 aacccatcag accacggtga taaagcccgt ggctcgtcaa gagttcgttg acttcgcgag
    31861 gggccggtcc ataatggagt ccatggaccc cgtcatcgcc cttcttaccg atttcggtac
    31921 ccgcgaccat tatgtcgcct gcatgaaggg cgttattcta cagatcaacc cgcgagccat
    31981 catcgaagac attacccacg agatcaaccc gcaggacatc taccacggcg cgtttgtgct
    32041 acgtcaatgc ctgccttggt ttccacccaa gacgatcttt gtggcggtgg ttgacccgac
    32101 ggtgggtacc aaccggcgga tcctggccgc tcgatacagc gatcgatatg tcctggctcc
    32161 ggacaacggc ttgctgacgc ttgtccaccg cgacgccgtg ctgcaggggg taaggtcggt
    32221 tgagaatcac caatacttcg ccgcacaggt ttcgaggaca ttccacgggc gagatatctt
    32281 tgcgccggtg gccgctcacc tgtccaaggg cgttgcgttt gatcatctgg gaccggtggt
    32341 cgacaggatg gaagtgttga gcctgtcccg gccgcggatc catcccgacg gaaccgttga
    32401 cggagaagtg gtcctgatcg accacttcgg caatctcatt accaacatcg cggtcactga
    32461 tctgacggcg gccaacatcc ggcgacacca cctgcacgtc acggttggcg agacggttat
    32521 cggacccctt cgggactcct atgccgacgt gccgcccggc caacccgtcg cgttataagg
    32581 cagctagcag atgctggtgt ttggcatcaa cggcggcaac gccgccaaga ctctgggagg
    32641 ggctcggggc acgcccgtca tccttcgcta agccagaccg cctttttgcg ggccgcaacc
    32701 ggagcgcacc gccatccaca aacatgcgcg cgggccgcat agatccctgg tcaatagggc
    32761 tctgaacaac ggcacgcgat cacacaacag agcagggcaa agacctgtcg gagtcatgtc
    32821 gcgttccgca tccgaggcga catggagtcg gggatggtca tgaaagagat gaaccggcta
    32881 gcgccggaag atcggactgc ggtcttccgc accgcgtagg cgggctggat cggccgtagc
    32941 gggcgcccgg cttcacattt gcctggtcgt cgcgggtttg cgatactgtg ctgccgcccc
    33001 tctgaccgtt ctgttgcgac tgcgggccat gaagggggta caagactctc aggcggaatc
    33061 ggatgagtgc tcaagactcc tggtcacgac gaaggtttct gtcaccacgg ggtctgggtg
    33121 catccaccgg cggactgatc gccgcgcttg tgccacagat ccccaaacga ccacgttcgg
    33181 cgggcaagta ctgccagcac tggtgtgttt ctcgccgggc catgggatgc gaattcaccg
    33241 tcatgctacc tccggatcaa cccagcccca tggcgtcggc tgaggcagcc ctcgacgaaa
    33301 tcggggagtt ggaagatctg ctgagcgttt atcgtgggga cagcgccgtt tcgcgtttga
    33361 accgccaggc cgccgaggcc cctgttcgag tcaactaccg gctttacgag ctgctcaagc
    33421 aagcggccca gttgacgtgg caaacggatg gggcctttga cgtcgcgacg ggcgcgatgg
    33481 tcaaggcgtg ggggttcctg cgaggaccca agcgggtgcc ttccgagact gaacgccggg
    33541 aggcattcgc ccggagcggc atgagacatg ttgttttcga cgatgaggcg atctccgttc
    33601 attacgccgt ccccggactt gagatcaacc tggggagcat cggcaaaggt tacggtatcg
    33661 accaggcgct taagcgaatg atggccgatt tcggcaccga ctgtgtgctg atccaaggag
    33721 gccgcagcag tgtgcgggcc gtcgggtctc cggcggacga tggtcggggc tggctgctcg
    33781 gtatccagga tccgctttcg ccggatcgtc agctcggcac ggtctatctg aaagaccgag
    33841 cgctggccac gtccagcgcg gtcaaccagt tttttgaggc acagggacac cgctatggcc
    33901 acctgatcga tccccggacg gggtggcccg ccgacggcct ggccagcgtc accgtcctgg
    33961 cacctgatgc cgctacggcc gatgctctgt ccacggcttt gtttatcatg gggctcgaca
    34021 aggcggccgc tttctgtcag aatcaccccg agattgccgc tctcctcgtc ccaaaccgga
    34081 atggggacga cggccaggac gcacggccac gtgtcgtgac ctttaaccta ccgccggaag
    34141 acgttagact gatgcccggc aacgacctgc cgggttcttg agagttgcct gacggagcga
    34201 tttcgccacc agagaattca taggtgaaga caagatcatg gctcagctaa gaccgacatc
    34261 acagcctgca tcgcagcaac cgcctccccc gacgccgcaa ctggcaaagc ctcagaccgt
    34321 cgcaacggtg cggatgttca ccgggtggac gattgccctg ctggtcatcc tccgaatctt
    34381 catcggctgg catttcttca ttgagggagt ctacaaacta tgccagcccg actggcgggc
    34441 gaccagctac ctggtggcct ccgcgggccc cttccgcgag tacttccgga aattcgtcaa
    34501 ggacgtcgat ggtcgtgaag ccctggcaat caacgatccc ggcattctcg aaggcaacga
    34561 actggatggc ccaaaggtcg ccgcactgag caaggcgctc gaggagagcc tggccgccag
    34621 actggacgag atttacgacc ggacggtcag tttttacgga ctggacagcg gccagcaaga
    34681 agaagccagg aagatcaagg agggaaaaaa ggccgagctg gacctgcttt gccgcgataa
    34741 ggacttcatc gcgcaaatct gggattacaa ggtccttctg gatgaaatct cgcggcagga
    34801 aaaggttgcg gacgcgacgg ttctggcttt cgagcgagag cgactgcagg atatgtacaa
    34861 gcgcaaggcc cggatccggg ccgcgctggt cgcgcgtgcg gataaaatca tgaccggcct
    34921 gccgacggcc gtgtacaagg cgaaacgcac cgcaacggac gacaagggcg cgaaggtgga
    34981 aaaagcactg ctgaccgagt cgcaactgag caaagggtac atgaaaacgg atttcagccc
    35041 gtcgaggttc agcgactttt cgaacaagtg ggggctgacc atcgtcggcg cgtgcctgat
    35101 cctcggattg ttcacccggc tggcgtgcct cggagccgcg ggcttgctgg caatgtatta
    35161 cctcgccatg ccgccgctgc cgggcgttcc cgaggggccg tccgaggggc actacctgat
    35221 cgtaaacaag aacctgattg aattggtgac ggtgctgatg ttggcaacca cgcgggtcgg
    35281 ccgatggggc ggccttgatg ggttgctggc cagcaaagcg aagccggcgg tttgacccac
    35341 aggatcaatg tttttcggaa aggctgatta tcaggagtct ccgtaaccgg agtcgactac
    35401 agagagcaaa aaggatctca acacatggta ctgaatgccc aacaacgtca gatcggtcga
    35461 gacaacttcg gggacgccag cagcgaggtc ttcaggatca cccggcgaca gatgctcagc
    35521 gctgggctgg tcatcccgac ggccgccgcc atgtactggg gatacggcaa gctcaaaggg
    35581 gcaccggtga aaaccgctct gatcggaacg ggcgaccagg gttgctacgc ccacatctca
    35641 caaagcaacc cggagtacgt cgatttcgta gccttctcgg acatccgtcc gtcgagccag
    35701 caacgtgccc gcgacgcgtt caaggcgctg tatggtcctg agaaggccaa caagatcagg
    35761 ctggaagagg actatcacaa gcttttcgac gacccggaca tcgagttggt catcatcgct
    35821 ttaccgctgc acctgcacgc gccggccacg atcgcggccc tgcaaaaggg caagcacgtc
    35881 ctttgtgaga aactcatggc taaaacggtc accgagtgca agcagatggt caacgcggcg
    35941 gcggcgaaca accgattcct ggccattggg catcagcggc actacagcta catgtatgcc
    36001 aacgccctgt cgctcatcaa gcagggcgtc gtgggggaca ttcgccacat tcacgcctac
    36061 tggcatcgta accagaccgg gttcgacagt tggagcaagg agatcccgcc ggaggaccgg
    36121 aaggtggaca ttgccaagta cgggtacgag agctacgagc agcttgtccg gtggcgaatt
    36181 gacaaccgca cgggcggcgg cctgatggtc gagttgggca gccatcagtt ggacgcggcc
    36241 agcattttcc tgaaccacaa gctgccgacg gagatggtcg ggaccggcgt gacctcctat
    36301 tttaaggacg ggcgcaccat cgcggatcac atcttcatgc agtatttgtt cggcgaccag
    36361 aatgttttca agagtctgcc cgaccgagcg aaggagccga caacggcaag cgctccggca
    36421 gaaccggttc tgttgcccct gccgtacacg accgacaaca cggtggtcac ctattcgtcg
    36481 atctcaacca atgcgttcga cggttacggg gagcaggtga acggatcgcg agcgacactc
    36541 atcatggacc gcgagatgga agcgtatctg ttctacgagg acttcaagga taagtccaag
    36601 gacacgcggg ttgacacccg catcgcatgg gccgagaacc gcatcggcaa gccggtgatg
    36661 tcgtcgggcg gcacggcggc ctggggcagc gcggtgggta cggcggacac gttgaccagc
    36721 cgcggctacc gcgaggagca ggaacacctg gcctggctga tccgcgagat cggcaagccc
//
LOCUS       DAOTPW010000016        36246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1264_length_36246_cov_5.117571, whole genome shotgun sequence.
ACCESSION   DAOTPW010000016 DAOTPW010000000
VERSION     DAOTPW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 36246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36246
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_36246_cov_5.117571"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="P5316_02530"
     CDS             <1..195
                     /locus_tag="P5316_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRR83861.1"
                     /translation="RLRTPLLSPVHDQRQGYVPNVVYTCGSMIHHDQLVIPYAVADSR
                     TSFAMVNVRELIRALLDSGP"
     gene            215..1474
                     /locus_tag="P5316_02535"
     CDS             215..1474
                     /locus_tag="P5316_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83862.1"
                     /translation="MSTRKLTRRKVLRAAAGTAIGLPYMITSSALGDAETAPAGERVT
                     LGHIGVGNRGKELLRGFMTCKGCQSVAVADAYSDRREAAAQMIGGKAYADFRELLARP
                     DIDAVVIATPDHWHVPIAMAAARAGKDAYVEKPLGVSIEQNLACRKLFREKKRVFQYG
                     TQQRSLKHCRFGCELVRSGRIGKVHTIEVTAPDGSAGGSTEPADVPDELDYDMWIGPA
                     PMKPYTEDRCVVPGTYFIYDYSIGYLGGWGAHPLDIMVWGSDADLAGPVTVEGTGVIP
                     DKGLYDTVIHWDLKVQMADGVRMTFKPGGDSTKYIGPDGWVRISRAGIDAEPKSLLES
                     KLGSGDVHLPVSERQDQNFIDAVKSRKPAISTLDDAVRSDIISHLCDIAIRTRSKVTW
                     DPKKEVIVDNEAASKMLTRPTREKWRL"
     gene            1611..1829
                     /locus_tag="P5316_02540"
     CDS             1611..1829
                     /locus_tag="P5316_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83863.1"
                     /translation="MTYRGRVKNGVVILDPPACLPEGAEVEVVPAQPEQPPPTLAERY
                     RDIIGIAEGLPADSSQNHDHYLYGAPKQ"
     gene            1860..2228
                     /locus_tag="P5316_02545"
     CDS             1860..2228
                     /locus_tag="P5316_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRR83864.1"
                     /translation="MAILIPDDANHEAAINLADHMTQPVITTEFVVLEVGNFLSATSV
                     RSRFATFLRVLREDPNTTVVPVSAELLRRAIELFLARPDKAWSVTDCTSFEVMREHGI
                     TDALTADHHFEQAGFTTLLK"
     gene            2543..2809
                     /locus_tag="P5316_02550"
                     /pseudo
     CDS             2543..2809
                     /locus_tag="P5316_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
     gene            2821..3066
                     /locus_tag="P5316_02555"
     CDS             2821..3066
                     /locus_tag="P5316_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRR83865.1"
                     /translation="MHQSIVSVRPNEDFSLAVTFEDGTEGVLDMKPYLDFGVFKKLQD
                     LEQFKRVRIAFDTVQWDCGADLDPEFIRASCRVTEKT"
     gene            complement(3381..4214)
                     /locus_tag="P5316_02560"
     CDS             complement(3381..4214)
                     /locus_tag="P5316_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HRR83866.1"
                     /translation="MENIYKPELMEIIQIQQHTSDVKSMRVKFADPERAKNFSFKVGQ
                     FGIFSAFGHGESTFNICSSSDWKDFIEFCFRRTGRVTEAFWQLEEGDTIGFRGPYGNA
                     YPMKEWEGKDLMFIGGGIAMPPIRCAIWYALEHREKYGDITIIYGARTVGDLVFRDEL
                     AEWAKRPKTRVIQTVDPGGETPDWKGEIGFVPTVVEKAGLTPKNAAALVIGPPIMIKF
                     TLPILAKMGLTDKDIYTSLENRMKCGVGKCGRCNAGPVYICKEGPVFTLEQVKTLPND
                     Y"
     gene            complement(4217..5236)
                     /locus_tag="P5316_02565"
     CDS             complement(4217..5236)
                     /locus_tag="P5316_02565"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR83867.1"
                     /translation="MSEIISKEDLLRLVDQWIGQGKRTAGPTRIKSDVVLYAPIRSSS
                     DVQLDGFIRPRNSIKEFIFPRHEKLYGYKVEGRKIQLFDAEPAVGEQLIIAARPCDAA
                     AFPILDHVFNWDYKDEFYNRRRQATTIVTLACKEYDRNCFCTSVGLGPAAESGSDAML
                     LDLGDGSYEVRCLTEKGKALFAGKTGTSDKTGTVPPGPKKIFTKESIDAFFAGGYDNP
                     EWQQMTLRCLGCGACAYTCPTCHCFDIVDEGNAAGGARVKNWDACQFCMFTAHASGHN
                     PRTNQGQRQRQRIYHKFEIYPTKFGEILCTGCGNCTRNCPVELGVRPVLETIETLSVA
                     GGTKA"
     gene            complement(5233..6078)
                     /locus_tag="P5316_02570"
     CDS             complement(5233..6078)
                     /locus_tag="P5316_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR83868.1"
                     /translation="MIQDELRQLCRRLLEDGTVQVVIGYGQSSDDGPTYPVFVTDAAD
                     VDRLVWNDQCFANLTTYLKRKEVKALGKPAIIVKGCDERALAVLEKESQIDRTQMVVI
                     GVACEGVGQPREPKCAACDVHMPRFSDHVLGQAANTPVEADRRYADLEAFMKRSPAER
                     MAYWMNELSRCFKCYACRATCPMCYCERCIVDKNRPQAIDTSPTLKGNFAWHIARAFH
                     LAGRCVGCDECTRVCPAGIDLRLLNMSLAKAAEDEFGYRAGVDREAEPIIGSYSLKDK
                     ESFIR"
     gene            complement(6075..6527)
                     /locus_tag="P5316_02575"
     CDS             complement(6075..6527)
                     /locus_tag="P5316_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRR83869.1"
                     /translation="MSEHAGDNGNGFEPKIVAYVCNWCTYLGADLAGTNRLAYPPNVR
                     IIRLPCTGRIDFNLIIKAFEMKADAVLVSGCHPGDCHYTAGNYHARRRWMLFRELLET
                     MGIDMNRIHFTWISAAEGRKFQQIITEITEKTRQMGPYMDYRKVCEAS"
     gene            complement(6524..8524)
                     /locus_tag="P5316_02580"
     CDS             complement(6524..8524)
                     /locus_tag="P5316_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HRR83870.1"
                     /translation="MARIGVFVCWCGENIARNVDCGKVSEAASRFPGVVCSVDYKYMC
                     SDPGQAMIRERIIKDRLTGVVVASCSPHMHLKTFRKAAEQEGLNPYLVEMANIREHCS
                     WVHHDKEKATAKAIELVRMAVAKVMYNHPLDPIRVPVTKRALVIGGGVAGIQAALDIA
                     DAGYEVVMVEREPSIGGKMAGLSETFPTLDCSQCILTPRMVEVGQHPKIRLHTYSEVQ
                     SVEGYVGNFKVNILKRARYVDMKKCTGCGACWNACPSKKTLSEFDFGMGTRTAIYVPF
                     PQAVPARPVIDANACLMLTKGKCGLCEKKCAAKAIMFDDKDQVVTEEVGAIVVATGYR
                     LYGIGKQQDSAKLAGYGEYGYDKYKDVIDSLQFERIVSASGPTNGEIKRPSDGKTPKT
                     VVFIQCVGSRDNAKGMAYCSKICCMYTAKHTMLYKHKVHDGEAHVFYMDIRAGGKNYD
                     EFVRRAIEQDGAKYHRGRVSKITEEDGKLIVRGVDTLANEPVTIEADLVVLASAMRPA
                     DGVEELAQKLNVGYDEYGFLSESHPKLRPVETNAAGVFVCGACQAPKDIPEAVAQASA
                     AASKVLGMFSKPELTREPEIAIVNEQTCAACFACVRTCPYNAIERAEIRDRKGNLVKR
                     TARVNPGLCMGCGTCVAICPSKSIELEGFTEQQIYAQIGSLV"
     gene            complement(8531..9391)
                     /locus_tag="P5316_02585"
     CDS             complement(8531..9391)
                     /locus_tag="P5316_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRR83871.1"
                     /translation="MTRVEPMEIGYYPGCALHGSSNDYERSVRACLNALGVNLRELDD
                     WICCGATAAHSLNQKLAVALPARSLGIAERDGYDDLLAPCPMCSMELLKAKMLISGDA
                     ALRKEMSEVVELPVEGRTSVLNLIQVFQRIGYDKVKEAAKVSLSGYKPACYYGCLLTR
                     PPEVLRFDDCEQPSSMETLLASLGAAPVEWNYKTECCGAGMTMCNEDTVLNLSHKILS
                     NAARHGANCVVVACPMCHVNLDMKQADVERRYGVQHGMMIYYLSDLVGLALGLDEEAL
                     GINRHYVSKR"
     gene            complement(9378..9956)
                     /locus_tag="P5316_02590"
     CDS             complement(9378..9956)
                     /locus_tag="P5316_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR83872.1"
                     /translation="MQKSPSMVDVIEELAHTRVHDCYQCGKCSAGCPMADHMDVIPSQ
                     IMRLVQMERLDKAMKAESIWLCVSCQTCSTRCPKSVNVAGVIDALRQLSVEQNVASPA
                     QQRTVVFQKAFLSNIRRNGRLKELELVGLFKVGAFVKDPSIAFLFKDSLLAPKMIARG
                     KFHPIGESVNDRDVVRRIFDRCMSSSGKNDKG"
     gene            complement(9956..11656)
                     /locus_tag="P5316_02595"
     CDS             complement(9956..11656)
                     /locus_tag="P5316_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83873.1"
                     /translation="MDSRRTLLVVADEKDPLGRGYLALLQACGAEIRTVCRTEEALRL
                     VASADVAGILLRTSRVKQDRDRCLRILAQKKPETPTILWADSRGGGLALSMASPGISQ
                     CLTEPVSPEEMAQALWRLIRRRNTVSGSLASGDPLFASKQGIRGDKYCFRNESWFQMS
                     HTGLAVIGVFPPVHPLGTFRSVSLPSTGEIVYQGLPLASIQAEGMAPRLVASPLTGVI
                     VDVNAELDCRPEALLEASCDESWLAWIFPTRLAEELPHCGLRHVVLVNSNPVSAAAQR
                     VALMKLGCEVKVVSGWAEAIQVLWERDSTIIVIDAASVAENGPEIAARLRSAAPEVRI
                     VVVASVGAQREAAYRRLGVFYYAIEPFADGEISDILGATFRPTVETLETCDWKPESYR
                     GIRIANREGREVRLLTSGGLLQQGNALGAWICRKLRQRLIPYEMISGQGGLTPAVIRD
                     EANRCDRLVVLAARDTGRLPGSLVRDSRGELVTVPPELAGKVIGLVVQPDPAGGAVGR
                     TEVLTAASLAEHVVVEMLLERPRLSRSGKTPSARRRRRGTAAAKRSRGLSSQVSGVTT
                     "
     gene            complement(11925..13352)
                     /locus_tag="P5316_02600"
     CDS             complement(11925..13352)
                     /locus_tag="P5316_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR83874.1"
                     /translation="MADMEKTFASQRVLVIDDEVVIGLSCRRCLEPDGHRVDCIEDPR
                     RGLEAALTGDYDVIFLDLMMPGLHGMDVLKQVKAAGVAAEVIIITGYSTVQSAVEAMK
                     QGAADYLSKPFTPDELRLVFRKAAERSALIRENAALRRALEIDKGFEGIIGESRSMER
                     VFGLIRRVAPTDGTVLVRGESGTGKEMVVRAIHRLSRRRNQPLLGCDCSSLAPTLLES
                     ELFGHVKGSFSGAIATKQGLFEVADKGTLFLDEVANLSLETQGKLLRVLETRRVRKVG
                     DTSEREVDIRLIAATNRDLMQMVKDGAFREDLYYRLNVVPIDLPPLRARQGDIPKLAM
                     VFLERCCRQNEISFKSFTPEAMAMMEAYSWPGNVRELRNVVERLAILCDSDRIEVRHL
                     PLEIRQAPISAGTAPLPKTWEEFKNLKQQVRDAAVQELERRFLSNALQQCEGNVSKAA
                     EHVGMQRTHFHALMRKHNLSSEPGG"
     gene            14200..14694
                     /gene="nuoE"
                     /locus_tag="P5316_02605"
     CDS             14200..14694
                     /gene="nuoE"
                     /locus_tag="P5316_02605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRR83875.1"
                     /translation="MVTPDIDAILAKHRDAKRDALIPILQDVQETQGYLSREAVVRIG
                     RHLGLPASKIYGVATFYNQFRFQPQGRFHIQVCRGTACHVKGSAAVLDAIRRELKIEP
                     GQTSRDGTFSLEVVACIGACGLAPVINISGEFHAGVTTQKVKGIIDSYRARAAGRGSN
                     DQAA"
     gene            14678..16645
                     /locus_tag="P5316_02610"
     CDS             14678..16645
                     /locus_tag="P5316_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRR83876.1"
                     /translation="MTKPPETISACCHEHAADQGYPTVLAHVMSSDPAQQDRLALLRH
                     DRVTRPTIYVGTGTCGLGAGARFTVGAIKEYLRQNNIEADLIGVGCIGLCSAEPIVDV
                     QLPGKARVSFKGITEDRVPTLLDAMMQGTVPAESVIGQHRTEFGEMWPDVPCLDEHPF
                     FAPQMRWVLANCGIMDPSSIDEYIARGGYTALARMLSAQTPEQVCDLVEASGLRGRGG
                     GGFPTGRKWKFARSAAGSGKYLVCNADEGDPGAFMDRAVIEGDPHRLLEGMAIAAYAI
                     GADKAYVYIRAEYPLAIERLKAAIKQARDYRLLGNNILGRGFSLEIVIKMGAGAFVCG
                     EETALIHSIEGKRGMPRPRPPFPAVRGLFDKPTIINNVETLANLPTLLTIGAERFSAV
                     GTKSSKGTKVFALSGKVARTGLVEVAMGTTIRRIVFDIGGGIPNGKAYKAVQIGGPSG
                     GCIPTQHLDIDVDYESLKTVGAMMGSGGLVVMDEDTCMVDVAKFFMDFIQRESCGKCI
                     PCREGTRRMLETLQRITRGRKSEKDIDALTRFQSVMHLPRLAEVIRDTSLCGLGQTAP
                     NPVLSTLRWFRDEYEAHIYERRCPAGVCQDLVTYRIIAEKCRGCTLCAKRCPVQAVMG
                     TPKSPHYIITDKCIACGSCLESCRFEAVVKE"
     gene            16758..18479
                     /locus_tag="P5316_02615"
     CDS             16758..18479
                     /locus_tag="P5316_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRR83877.1"
                     /translation="MITIEVNGRPVEAEPGEMLLAALRRAGIKVPTLCQIEGLFPTGA
                     CRMCVVEVEGQRGLVPSCAFPVSEGLKVQTHSPRALRARKTIVELLLANHPDDCLYCV
                     RNENCDLQRLAAELGVRSRRFSGGKNRHALDTSSPSLVRDPAKCILCGKCVRVCEEIQ
                     GVAAIDFIGRGSKTTVGTAFNEGVNVSSCVYCGQCVRVCPTGALREQSHMKEVADALN
                     HPDLMVVVQHAPAISVTLGEEFGMPPGSDVNGAMTTALRLLGFKRVFDTSFSADLTIM
                     EEASELVHRIKNGGRLPMMTSCSPAWIKFVETFYPDLMPNLSTCKSPQQMLGAVIKSY
                     FAEREGIDPARIFSVSIMPCTAKKFEAGRPEMCRNGLPDIDAVLTTRELARMIRMRGL
                     DLRELPGDTADTPFGERTTAGKLFGASGGVMEAAIRTAHYLITGKELADLTVQPVRGL
                     AGVKEARLQIAGMDIGVAVVSGLANARRLLDQILAGRKDLHFIEVMSCPGGCVAGGGQ
                     PINTDMDAVRTRMQALYRIDSVESRRTSHGNASVRRIYEEYLGRPLSERSHHLLHTHY
                     VKREVLA"
     gene            18498..20564
                     /locus_tag="P5316_02620"
     CDS             18498..20564
                     /locus_tag="P5316_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRR83878.1"
                     /translation="MGTAFISTIKEHCRMCYTCVRECPAKAIRIVDGQAQVIGDRCIA
                     CGNCVKVCSQGAKEVRSSIKEVEALLASGRKVAACVAPSFPAEFVDADPHQVVGMLWA
                     LGFELVNEVAFGADLVAREYRRLLSRNDDKRYITTSCPAIVGYVERYYPDLLDSLTPV
                     VSPMIATARALRQLHGNDLKIVFIGPCIAKKAEAESKPVAGEIDAVLTFPELREMLAA
                     RNIRPDNVEPCEFDPPHGATGAMFPVSRGVLQAADIREDLMTGEVVATAGRMHTLEAI
                     KEFASGDLGARLLEVLACEGCIMGAGISNDLPLFNRRRHMRKYFCKRIESLDLARWHE
                     DMERFAGLDLRRSYVANDQRIPTPADEQLNQILIRMGKFSPKDELNCGACGYDTCMEH
                     AIAIYKGLAETEMCLPYTIEKLRQTIKELAASHEQLASTQEALIQAEKLASMGQLAAG
                     IAHEVNNPLGVVLMYAHLMLEEYRDKTKMREDLAMIAEQADRCKKIVAGLLHFARQNK
                     VVRYPTDVRELVRRSIKTFTIPPNVTVKIDTEIADPVAEIDRDQIIQVLTNLISNAVA
                     AMEKGGTLTVRVTGDADNIRFVVGDTGVGIPEQNMKKIFEPFFTTKQIGKGTGLGLAV
                     TYGIVKMHCGDIRVESNADPAAGPTGTTFTVTLPRREQREMNQVSGTEMIRGLGQVES
                     APTGQN"
     gene            20589..20990
                     /locus_tag="P5316_02625"
     CDS             20589..20990
                     /locus_tag="P5316_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR83879.1"
                     /translation="MADTKKTILLVDDDADFLMQEEIALKAGGYDVLTADSGKRAEEI
                     IARTRPDAAIVDVMMEDTDAGFMLCYHIKKKDPSIPVIMVTSVTGETGLDFDAATDEE
                     KSWIKADAFLAKPIRIEQLEAELTRLLKDKT"
     gene            20987..22162
                     /locus_tag="P5316_02630"
     CDS             20987..22162
                     /locus_tag="P5316_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR83880.1"
                     /translation="MMDTLRILVTDDERGMRLGVERTLRDFRVMVPDVHEEVAFVVDQ
                     AETGEQAIEQMATRPPDILLLDHKLPGISGLEVLDRVSGTRPELLTIMITAYASIETA
                     VVATKRGAYDFLAKPFTPDELKSTVRKAAIRIMLARQARKLAEEKRQVRFQFIRVLGH
                     ELKAPLNAIDGFLQLIKNHTLGGDVGAYDEIVGRCSVRVDHMRKLIVDLLDMTRIESG
                     QKSRDLGTVDVREIARTSIETILPTAQPRNIAIELHADGPVTMTADRGEIEMILNNLI
                     SNAVKYNRDNGRVDVRVDRSGQVVTIAVTDTGIGMSKEETAKLFGEFVRIKNARTRSI
                     LGSGLGLSIVKRLAMLYDGSATVESEPDVGSTFTVVLKDSPGPPPSAVPAATTMNEA"
     gene            22167..23576
                     /gene="hydG"
                     /locus_tag="P5316_02635"
     CDS             22167..23576
                     /gene="hydG"
                     /locus_tag="P5316_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRR83881.1"
                     /translation="MRQETVGLPASDIIRERRIEEILAHNRDQDASLVREILAKARQL
                     KGLPMEDVAVLAEISDPELLAELFATARQVKQDIYGMRLVLFAPLYVSNLCGNNCLYC
                     AFRSGNKSIKRRALTQDEIARETRILVEQGHKRVLLVAGESYPNEGFDYILRAIKTVY
                     SVRSGRGEIRRVNVNIAPLELDQFRQLKDMGIGTYQLFQETYHRETYARVHTGGRKRD
                     YDWRVTAMDRAMEAGIDDVGIGVLFGLFDWRFEILALLQHVQHLEAAFGVGPHTISVP
                     RLEPATGADLASHPPHPVSDVDFRKIVAILRLAVPYTGIIMSTRETPNLRRETFALGV
                     SQISAGSRTNPGGYTEDEDIDASQFCLGDHRPLDEVIRDVAQLGYIPSFCTACYRLGR
                     TGQDFMDLAKPGEIKHHCDPNGLSTFLEYLLDYASPATREVGEQRIASVLAEMDDRAR
                     RTAEKLIEQVRAGKRDVFV"
     gene            23613..25070
                     /locus_tag="P5316_02640"
     CDS             23613..25070
                     /locus_tag="P5316_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRR83882.1"
                     /translation="MSSAWRTEHDLIGSLPVPADALYGIHTARALRHFTLAGRPVHPQ
                     MIRAYGTVKLACAMTNRALGCWTDDPAKADAIERACREMGEGLLDGGVQVDLLQGGAG
                     TSTNMNVNEVLANRALQILGEPLGEYRRVSPLDDINLHQSTNDTYPTALRLAAIRMLA
                     GLEERVVGLQEAFQAAEKRFAHVVKVGRTQMQDAVLITLGREMAAYAEAFSRDRWRIY
                     KCQERLRVVNLGGTAIGTGLAAPRDFIFRVAETLRDLTGIGFARAENLVDATQNADVF
                     VEVSGILKACAASLLKICTDLRFMSSGPEAGVGEIRLPQLQAGSSIMPGKVNPVIPEA
                     VSQAAMLVMGHDATLTMACAAGNLELNPFLPLVACCLLESIELLERSCDALRRYCVEG
                     IEADEARCRRHVDAATATVTALLPALGYEQACRIALKARQGGRCIRDVVVEEGLLTAQ
                     AFDEMVSPEAVCRLGSPMIRRPNAEYGPVGQGGAP"
     gene            25067..26338
                     /gene="hydF"
                     /locus_tag="P5316_02645"
     CDS             25067..26338
                     /gene="hydF"
                     /locus_tag="P5316_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRR83883.1"
                     /translation="MTTSTPKGFRLHIGIFGRRNVGKSSLLNAVTRQNVSIVSEFAGT
                     TTDPVEKPMELLPLGPVLFIDTAGVDDAGALGELRVGKTRQVFDRTDLGVIVAEPSIW
                     SDFEERILNELASRQIPVLVVFNKCDVAMPDAAVVERLAARKVPVVRTAATSGQGILD
                     FRQALLDNAPADFVNNPRILADIVGPGEMAVLVVPIDKEAPKGRLILPQVQAIRDLLD
                     GDAYCMVVKERELRNALDRLKSPPKLVVTDSQAFLKVAADTPAEVMLTSFSILFARFK
                     GDLITQVEGTLAVDSLVAGDRILVAEACSHHPIGEDIGRVKIPRWLTQYVGGKIEFVT
                     VQGHDFPEDLSSYKLIIHCGACMWNRREMLSRIMRCRQARIPITNYGLVIAHSLGILE
                     RALQPFPAALEILRRARDFGAAASRQAQGLP"
     gene            26335..27408
                     /gene="hydE"
                     /locus_tag="P5316_02650"
     CDS             26335..27408
                     /gene="hydE"
                     /locus_tag="P5316_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRR83884.1"
                     /translation="MNRTPPAAAPTPTREELLRWLREDDASRLGQLWTMADETRRRNV
                     GDAVHLRGLIEISNHCVRECAYCGLRAPNRSLRRYRMTADEIMACTAAAVEFGYGTVV
                     LQSGEDDGIRQQWLADVIRKIKSETSLAVTLSLGERPEEDLAAWRQAGADRYLLRFET
                     SDPVLYKLIHPPRPGRLSDRFAILNQLRELGYEVGSGVMIGIPGQTYDSLADDLEAFR
                     RLDLDMIGVGPYISHPATPLAKHSGLRPIPPEQQVPNTELMTYKVLALTRLVRPDANI
                     PSTTALATLNKDAGRELGLSRGANVVMPNLTPPQYRTLYEIYPSKACLFENAEDCRSC
                     LKNRILAMGRTIGQGPGSRRQTA"
     gene            27486..27929
                     /locus_tag="P5316_02655"
     CDS             27486..27929
                     /locus_tag="P5316_02655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRR83885.1"
                     /translation="MDMQQIARFFDRDKLAKHLDIELVDVSPGQATTRMTIQDKHLNG
                     VNITHGGAIFSLADFAFAVASNSHGTLAVAINANISFLKATTSGVLTARAREVARSKR
                     LATYQIEVTDEEGATVAGFQGTVYRKQEPLPLEEDLTPPSSGGGR"
     gene            complement(27898..29223)
                     /locus_tag="P5316_02660"
     CDS             complement(27898..29223)
                     /locus_tag="P5316_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRR83886.1"
                     /translation="MAQFKVENRIWDPAETMPRAELRRLQLERLKDCVRRVADVPFYR
                     EAFASTGVSADSIRSLDDLRRLPFTTKDDLRRYYPLGFLAVEREKLVRIHGSSGTTGK
                     PTFVAYTAQDMDIWSNLCARFLVAGGLRPEHFVQIAFGYGLFTGGFGLHQGVEKVGAA
                     IVPASAGNTPRQLMLICDLNVQVLICTPSYALNIAEAARDQGMTPEQIPLKFGHFGAE
                     PWTEDMRTRIEQELGIVAFNNYGLSEVIGPGVSGECTLQQGMHIQEDHFIVECVDPDT
                     LEPVATGEAGELVFTTITKEALPLIRYRTRDIARLDDTPCRCGRTTVRMSRVIGRTDD
                     MLIIRGVNVFPSQIEEALLRVEGTSPHYQIELSRPGALDQVTVRVEVRPEDFSDRMRE
                     MQALRDRIDREIHAITGLRMNVELVKPQSLERFQGKAKRVIDRRQMKVE"
     gene            complement(29310..29750)
                     /locus_tag="P5316_02665"
     CDS             complement(29310..29750)
                     /locus_tag="P5316_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HRR83887.1"
                     /translation="MKLHQLSLFIENKPRQLRSPIRILAEAGVNILTLCLADTQQFGI
                     LRMIVQDWQKAKAALEKGGCVVNVTEVIALEVPDHPGGLDDILAVIEEFSLNIEYTYS
                     FTFRRGNRAVLVFRFEDMDAAVEAFQAKGINPVSSVEIYEQANV"
     gene            complement(29765..31099)
                     /locus_tag="P5316_02670"
     CDS             complement(29765..31099)
                     /locus_tag="P5316_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRR83888.1"
                     /translation="MYKEMWNDLPGGFHPASAPDFLPIPQLRDLQFRRLTAVVRRAYD
                     HVELFRQRMQERGLTPADIRSLDDVAKMPFMVKTDLRDTYPFGLFASPMQEIVRLHAS
                     SGTTGKPIVVAYTQEDLEVWASVMVRTFAACGLHRGDIIQNAYGYGLFTGGLGAHYGG
                     EALGATVIPISGGNTDRQIMVMKDFGVTAICCTPTYFIHLIERAKELGVDIRQLPLRA
                     GVFGAEPWSEAMRQYIERESGIKAMDIYGLSEIIGPGVASETPYQEGLFIFEDHFYPE
                     IIDPETCEVLPEGQEGELVLTTLSKKAMPVIRYRTRDITAFISGPSRCGRTIRRIRRI
                     SRRSDDMFIIRGVNVYPSQIEAALLAVEGTLPHYQIILTREKGLDQITVEVEVLAEWF
                     SDKVRSLEALKQKLASSIEHIIGIRVNLRLVEPHTIERSQGKAKRVIDRRNE"
     gene            complement(31129..31647)
                     /locus_tag="P5316_02675"
     CDS             complement(31129..31647)
                     /locus_tag="P5316_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRR83889.1"
                     /translation="MTEENVASGVTNVVIAGLGGQGVLKASDILAQVAFDEGLDVKKS
                     EVHGMAQRGGSVTSDVRFGAEVFSPMVPAGEADFVVVMAPEEVEGSKRHLKPGGVMIV
                     PDDVPVTSLDSPRSLNVALLGVLSAYLPLPESTWLSAVKGSLAEKLHEANIKAFLLGR
                     SVGREKQASSAC"
     gene            complement(31648..33249)
                     /locus_tag="P5316_02680"
     CDS             complement(31648..33249)
                     /locus_tag="P5316_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRR83890.1"
                     /translation="MQQTDRVLLSGNEAVALAARRAGVALGSGYPGTPSTEILETFGE
                     MGGKAQWAPNEKVALEVALGAAFGGARALVTMKHVGLNVAADPLFTAAYTGVVGALLV
                     VSADDPGMASSQNEQDNRRYAAAAAVPMLEPSDSQEAFDFTLRAVEISEKWKIPVILR
                     MTTRVCHSKTVVRPVGEMPAPAPRFEHDIAGRVMIPAHARPAHRRLREKIAQIAAWNE
                     TSGLHRVIDGDHGLGIITSGISYVHVREAAPQASVLKLGMTHPLPMETARKFAAGVDR
                     CLVIEEGDPYLVESLRAGGIEVAGKPEMYRFGELNVARVRRILAGDTTPEPAPPRGKP
                     PALCLSCPHRSVFGVLSKLKCIVAGDIGCYTLGVLPPYQAMDSCVCMGASIGVGLGLR
                     QVLPPEQARRVVSVIGDSTFVHSGITGLVEMVYNPPPTGHVILILDNGTTAMTGLQEH
                     PGTGRTLMHEPTGKVSYEDLARSLGIRNVHVVDATKDESAFEKLVSDSLAADELTLII
                     ARRPCLLATARIKAYEQAASSGGNK"
     gene            complement(33250..33750)
                     /locus_tag="P5316_02685"
     CDS             complement(33250..33750)
                     /locus_tag="P5316_02685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRR83891.1"
                     /translation="MLKERGSIGRKGAERHRRANLGADEECGVAGDGHQVRFRVRYSE
                     TDQMGTFYNSRVLEWFECGRTEWLRSVGIPYAEMEADGTYLPVVEAHVRYLGRARYDD
                     ELEMCVSAGMSGKASVRFDVRIRQAHNGQGVAEGYTVHAITGADGKPVRPPSWLVRIF
                     SASSEQ"
     gene            complement(34133..35572)
                     /locus_tag="P5316_02690"
     CDS             complement(34133..35572)
                     /locus_tag="P5316_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRR83892.1"
                     /translation="MRSKWTVVTSVFLVALLVACTARMATAQPATQPAAAAEDPFDEF
                     VEKTKNPFPGFNWGMDLRNRWEYLNNTGFDKHATGHETSYLRFRGRWWGTLQPLKPLN
                     FEDLEFHLKLTWEGRSWCEHPTISDWEQARDQVLFDNMYLKYKKAFGLPLTITAGRQD
                     LPFAWLTGDGTPLDGSRTFFVDGVRFEYDAEDIKTKFDVAWFEVDAQWDHWFPRIANK
                     RVPALATEQDEQGVMVYVTNKSLAKTQIDGYFFYKHNEAELIPVRNNPNTDEPRRSGD
                     NADIYTFGGRVAGDITDNWKYDVQLAGQFGQRDGRDICALGTLNSLTYHFNDKYKNQV
                     YLEYEFTSGDDPSTRGTDERFDILWGRYPRWTEGYGFSYGIENRIWDLGNTHRVGPGW
                     RCKPTKDMDLGLYYQFLFADENTYAGRAGFSNSGCFRGQILVPYFKYVFNKHAFLRVM
                     PEFVFPGNYYTDARNDPLTFLRLELNLTW"
     gene            36059..>36246
                     /locus_tag="P5316_02695"
     CDS             36059..>36246
                     /locus_tag="P5316_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRR83893.1"
                     /translation="MDLKTSTTIRLTCAPGLAPLLETEVRQLGWPITSVDDAGINTTG
                     TLIDAMRLNLHLRTAFYVL"
BASE COUNT         7093 a        11650 c        10721 g         6782 t
ORIGIN      
        1 cgcctccgca cgccgctgct gagtccggtt cacgaccagc gacaaggcta cgtgccgaat
       61 gtcgtgtaca cctgcggttc gatgattcac cacgatcagc tcgtgattcc ctatgccgtc
      121 gccgattcac ggacatcctt cgcgatggtc aacgtccgcg agctgatccg tgccctgttg
      181 gacagcggcc cgtgattcat gaggaaacct cacgatgagc acccggaagc tgacgagacg
      241 aaaggttctc agggccgccg cgggaaccgc gatcggtctg ccgtacatga tcacctcatc
      301 ggcgttgggc gacgcagaaa cggcgccggc cggcgagcgg gtgacgctgg ggcacatcgg
      361 cgtcggaaac cgcggaaagg agctcttgcg aggcttcatg acctgcaagg gctgccagag
      421 cgtggccgtc gccgatgctt acagtgaccg gcgggaagcg gcggctcaga tgatcggcgg
      481 gaaggcttat gcggatttcc gcgagttgtt ggcccgtccg gacatcgacg cggtggtgat
      541 cgcaacgccc gaccactggc atgtgcccat cgcgatggcg gccgctcggg ccggcaagga
      601 tgcctatgtc gaaaagcccc tgggcgtcag catcgagcag aacctggcct gccggaagct
      661 ctttcgcgag aagaagcggg ttttccagta cggcacgcaa cagcggagcc tcaagcactg
      721 ccgcttcggc tgcgagctgg tgcgcagcgg gcggatcggc aaggtccaca cgatcgaggt
      781 gaccgctccg gacggcagcg ccggcggttc cacggaaccc gcggacgttc ccgacgagct
      841 ggactacgac atgtggatcg gtccggctcc gatgaaaccc tacaccgaag accgctgtgt
      901 cgttcccggc acgtatttca tctatgacta ctcgatcggc tatctgggcg gctggggggc
      961 tcatccgctg gacatcatgg tctggggttc ggatgccgat ctggccgggc cggtgacggt
     1021 cgagggaacg ggggtcattc cggacaaggg cctgtatgac acggtgattc attgggatct
     1081 caaggttcag atggccgacg gcgtccggat gacgttcaag ccagggggcg attccacaaa
     1141 atacattggc cccgacggct gggtgcgcat ctcgcgagca ggcatcgacg cggagccgaa
     1201 atcgctgttg gaatccaaac tcggctccgg cgacgtgcat cttcccgtaa gcgagcgaca
     1261 ggatcagaac ttcattgacg cggtcaagtc ccgcaaaccg gcgatcagca ctctcgacga
     1321 tgccgttcgg tccgacatca tcagccacct ctgcgacatt gccatccgca ccagaagcaa
     1381 ggtgacctgg gatcccaaga aggaagtcat cgtcgataac gaggccgcct cgaaaatgct
     1441 gacccggccg acgcgagaga agtggaggct ttgaaccagg gtctgtgcca gttcgggcgg
     1501 ctcttttaga accgcgaccg tcagggagca gccccctgga gccgttgcgg ctgcgagaga
     1561 gggtgcaaac gggtcggacc gaaggtataa taccaacaag gaggtacgcc atgacctatc
     1621 gcggacgtgt caagaacggc gtggtcatcc ttgatccgcc ggcctgcttg ccggaaggag
     1681 ccgaggtgga ggtcgtgcct gcacagccgg agcagccccc gccgacactg gcggagcgtt
     1741 accgggacat cataggcatc gccgagggcc tgccagccga ttcatctcag aatcacgatc
     1801 actacctgta cggtgcccct aagcaatgag cccctgcttc gccgacagct cgtacttcct
     1861 tggccatact cattcccgat gacgcgaatc atgaggcggc catcaacctc gcggaccaca
     1921 tgacgcagcc cgtcataaca actgagttcg tggtgcttga ggtgggcaat ttcttgtcgg
     1981 caacgtcggt acgaagcagg ttcgccactt tccttcgggt cctgcgtgag gatccgaaca
     2041 ccaccgtagt cccggtgtcg gccgaactgc ttcgccgagc cattgagctg tttcttgccc
     2101 ggcccgacaa agcctggtcg gtcactgact gcacatcgtt cgaggtcatg cgagaacacg
     2161 gcatcaccga tgctctcacg gccgatcacc atttcgagca ggcggggttc acgaccctgc
     2221 tcaagtgatc tgccggggcc cggacaatca gcgatgtccc ctttggggcc cctccgacgg
     2281 ccgtcctccc gcgtggccat gaacggaccg accttcttgt ttagtccctt ccgtcgcgac
     2341 tggtgcgcgt tttgcgcgca tgtttctgga ccgcggtacg cttgggttgc ggcccgcaaa
     2401 aaggcggtcc gccttagaga ataggaccgg gagtacactg taccagggaa ccgacccgac
     2461 aaggacgagg gccgggggga tgacagccgc tggattgccg atggccgtca ggcgatctca
     2521 accggagaag gagaacagcg atatgccgac tatatcaatg ttttacggca tcgttattcg
     2581 gctctatttc gcgccgggcg agcataatcc cccgcacttt cacacgttct agaacgagta
     2641 caaggcttcc gtggacatcc gcacgggcga catcatcgag ggctcccttc cccctcggca
     2701 gcgcaagctt gttgcagcat gggccgagct gcaccaagag gatttgatgg caaactggaa
     2761 cctggtcatg aacggtgatg aaccgttcag gattcagcct ctgcagtaga ggacgcaccg
     2821 atgcatcagt caatcgtgag cgttcgtccg aacgaggact tctctctcgc tgtcactttc
     2881 gaggatggca ccgagggcgt tcttgacatg aaaccctatc tggacttcgg cgtgtttaag
     2941 aaactccagg acctggagca attcaaacgg gtccgcatcg ctttcgacac ggtccagtgg
     3001 gactgcgggg cggatctgga tcccgagttc atccgtgcga gttgcagggt gaccgagaag
     3061 acgtgaaccc cgccgcgagc gcggtccgtc gccggcgact gctgaaccgc tgaatacgcg
     3121 aaccacgcga aagaagatac catccccggt cccctttcgc gtattgggcg tatttcgcgg
     3181 ttgcagggtg ccccatgccg cgggggtggg gaaccgaatc gtcaggctgg gcaatcgtag
     3241 agattactga tcgatttggt agtcgagcag attgcgttcc caccgttgcg tgacgaggca
     3301 cccgccgtag tctgccggtt gagtgccgcc ggggcaagcc cggtggctcg cttgtagggg
     3361 aacttggcgc cccgggccga tcaataatcg ttcggcagcg tcttgacctg ctcgagcgtg
     3421 aagaccgggc cttccttgca gatgtagacc gggccggcgt tgcagcggcc gcacttgccg
     3481 acgccgcact tcatgcggtt ttcgaggctg gtgtagatgt ccttgtcggt caggcccatc
     3541 ttggccagga tcggcagcgt gaacttgatc atgatcggcg ggccgatgac cagggcggcg
     3601 gcgttcttgg gagtcagacc ggccttctcg acgaccgtcg ggacgaagcc gatctcgccc
     3661 ttccagtcgg gcgtctcgcc gccggggtcg accgtctgga tcacgcgcgt cttggggcgc
     3721 ttggcccact ccgccagttc gtcgcgaaaa accagatccc ccaccgtgcg ggcgccgtag
     3781 atgatggtga tgtcgccgta cttctcacgg tgttccagcg cgtaccagat ggcgcagcgg
     3841 atcggcggca tggcgatgcc gccgccgatg aacatcaggt ccttgccttc ccactccttc
     3901 atcgggtagg cgttgccgta gggcccgcgg aaaccgatgg tgtcgccttc ctcgagctgc
     3961 cagaatgcct cggtcacccg tccggtgcgg cggaaacaga actcgatgaa gtccttccag
     4021 tcggagctcg aacagatgtt gaacgtcgac tcgccgtggc cgaacgccga gaagatgccg
     4081 aattgcccca ccttgaagga gaagttcttg gcccgctccg gatcggcgaa cttcactcgc
     4141 atcgacttga cgtcgctggt gtgctgttga atctggatga tttccatcag ctccggcttg
     4201 tagatattct ccatgatcac gctttggtcc cacctgcaac ggagagggtc tcaatggtct
     4261 ccagaacggg gcgaacgccc agctccacgg ggcagttcct cgtgcagttg ccgcagcccg
     4321 tacacaggat ctcgccgaac ttggtcgggt agatctcgaa cttgtgatag atccgttggc
     4381 gctggcgttg gccctggttg gttcgtgggt tgtggcccga ggcatgcgcg gtgaacatgc
     4441 agaactggca tgcgtcccag ttcttgaccc gcgccccgcc ggcggcgttg ccttcgtcga
     4501 cgatgtcgaa acagtggcac gtcggacagg tgtacgcgca cgccccacac cccaggcaac
     4561 gcagggtcat ctgctgccat tcggggttat cataaccgcc ggcgaaaaac gcgtcgatcg
     4621 actccttggt gaagatcttc ttcggaccgg gcggaaccgt tccggtcttg tccgatgtcc
     4681 cggtcttgcc cgcaaacagg gccttgccct tctcggtcag acagcgaacc tcatagctgc
     4741 cgtcgccgag atccagcaac atcgcatccg agcccgactc ggcggcgggt ccgagcccca
     4801 cggacgtgca aaagcaattc ctgtcgtatt ccttgcacgc caaggtgaca atcgtagtcg
     4861 cctgccggcg acggttgtag aactcgtcct tgtagtccca gttgaacacg tggtcgagaa
     4921 tcggaaacgc tgcggcatcg caaggccgcg cggcgatgat cagttgttcg cccacggccg
     4981 gctctgcgtc aaagagctga atcttgcgac cttccacctt gtagccgtag agcttctcgt
     5041 ggcggggaaa gatgaactcc ttgatcgagt tgcgggggcg gatgaacccg tccaactgca
     5101 cgtcgctgga agagcggatc ggggcataca gaaccacgtc ggacttgatc cgggtcggcc
     5161 cggccgtccg cttgccctgc ccgatccatt gatcgaccag ccgcaataga tcttctttcg
     5221 agatgatctc gctcaccgga tgaaactctc cttatccttg agcgagtacg aaccgatgat
     5281 cggctcggcc tcgcggtcca ctccagcccg gtagccgaac tcgtcctcag ccgccttggc
     5341 cagggacata ttcagcagcc gcaaatcgat gcccgccgga cagacgcgcg tgcactcatc
     5401 gcacccgacg caacggcccg ccagatgaaa cgcccgggcg atgtgccagg cgaaattgcc
     5461 cttgagcgtc ggcgacgtat cgatggcctg gggacggttc ttgtccacga tgcatcgttc
     5521 acagtagcac atcgggcagg tcgcccggca ggcatagcac ttgaagcatc gcgacagctc
     5581 gttcatccag taggccatcc gctcggccgg tgacctcttc atgaacgcct ccaggtcggc
     5641 ataccggcgg tcggcctcga ccggcgtatt cgcggcctgc ccaaggacat gatccgaaaa
     5701 cctcggcatg tgtacgtcgc aggcggcgca tttgggctcg cgcggttggc cgacgccctc
     5761 gcaggccacg ccgatcacca ccatttgcgt gcggtcgatc tgcgattcct tctccagcac
     5821 cgccagggcc cgctcgtcgc aacctttgac aatgatcgcc ggcttgccca gcgccttgac
     5881 ctccttgcgt ttcagataag tggtcaggtt ggcgaaacac tgatcgttcc agaccagccg
     5941 gtcgacgtca gccgcgtcgg tgacgaatac cgggtacgtt ggaccgtcgt cggaggactg
     6001 tccgtacccg atcaccacct ggacggtgcc gtcttcaagc aaccggcgac agagttgacg
     6061 cagttcgtcc tgtatcacga cgcctcacac accttccgat agtccatgta aggacccatt
     6121 tgccgggtct tctcggtaat ctccgtaatg atctgctgga acttgcgacc ctcagccgcg
     6181 gatatccagg tgaagtgaat gcggttcatg tcgatgccca tggtttccag cagctcgcga
     6241 aacagcatcc aacggcggcg agcgtgatag ttgccggcgg tgtagtggca atctccgggg
     6301 tgacacccgg aaaccagcac ggcgtcggcc ttcatctcga acgccttgat gatcaggttg
     6361 aaatcgatac ggccggtgca gggcaggcgg atgatccgca cgttcggcgg atatgccagc
     6421 cggttggtcc ccgccaggtc ggcgcccagg taggtgcacc agttgcacac gtaggcgacg
     6481 atcttgggct cgaagccgtt tccgttgtct ccagcatgct cactcatacg aggctcccga
     6541 tctgggcgta aatctgctgc tcggtgaagc cctccagttc aatgctcttc gacgggcaga
     6601 tcgcgacgca cgtgccgcaa cccatgcaca ggccggggtt cacgcgggcc gtgcgtttga
     6661 ccaggttgcc cttgcggtcg cgaatctccg cgcgctcgat ggcgttgtac gggcatgttc
     6721 gcacgcaggc gaaacacgcc gcgcacgtct gctcgttgac gatggcaatt tccggctcgc
     6781 gggtcagttc gggcttgctg aacatgccca ggaccttgct ggccgccgcg gaggcctgag
     6841 ccacggcctc gggaatgtcc ttgggggcct ggcaggcacc gcagacgaac acgccggcgg
     6901 cattggtctc gacggggcga agcttggggt gcgactcgga cagaaagccg tactcgtcgt
     6961 agccgacgtt gagcttctga gccagttcct cgacgccgtc ggccggccgc atggcgctgg
     7021 ccagcaccac caggtccgct tcgatcgtca ccggttcatt ggccagagtg tcaacgccgc
     7081 ggacgatcag cttgccgtcc tcttcggtga tcttcgacac gcggccgcga tgatacttgg
     7141 ccccgtcctg ttcgatcgcc cggcggacga actcgtcgta gttcttgccg ccggcccgga
     7201 tgtccatgta aaaaacgtgg gcctcgccat cgtgcacctt gtgcttgtaa agcatggtgt
     7261 gcttggcggt gtacatgcaa cagatcttcg agcaataggc catgcctttg gcgttgtcgc
     7321 gcgagcccac gcactggatg aaaacgacgg tcttgggcgt cttgccgtcc gacggccgct
     7381 tgatttcgcc gttggtcggt cccgacgcgg acacaatccg ctcaaactgc agcgagtcga
     7441 tcacgtcctt gtacttgtcg tagccgtact cgccgtagcc ggccagtttg gcgctgtcct
     7501 gctgcttgcc gatgccgtag agcctgtaac cggtggcgac cacgatcgcc ccgacctcct
     7561 cggtcaccac ctggtctttg tcgtcgaaca tgatcgcctt ggcggcgcat ttcttttcgc
     7621 acaggccgca cttgcccttg gtcagcatga ggcaggcgtt ggcgtcgatc accggcctcg
     7681 ccggcaccgc ctgcgggaac ggcacataga tcgccgtccg cgtgcccatg ccgaaatcga
     7741 actcgctgag cgtcttcttc gacggacagg cgttccagca ggccccgcag cccgtgcact
     7801 tcttcatatc cacgtagcgg gcccgcttga ggatgttcac cttgaagttg ccgacgtagc
     7861 cttcgacgct ctgaacctcc gagtaggtgt gcagccggat cttcggatgc tggcccactt
     7921 caaccattcg aggcgtgaga atgcactgtg agcagtccag cgtcggaaaa gtctcggaca
     7981 aaccggccat cttgccgcca atggacggct cgcgctcgac catcaccact tcgtaaccgg
     8041 cgtcggcgat gtccagggcc gcctgaatgc ccgccacgcc tccgccgatc accagggccc
     8101 gcttggtcac cggcactcgg atcggatcaa gaggatggtt gtacatcact ttggcgacgg
     8161 ccatacggac cagttcaatg gccttggccg tggctttttc cttgtcatgg tgcacccacg
     8221 agcagtgctc ccggatgtta gccatctcga ccagatacgg attcagcccc tcttgctcgg
     8281 ccgccttgcg gaacgtcttg aggtgcatgt gcggcgaaca cgaagcgacc accacgccgg
     8341 tcagcctgtc cttgatgatt cgttcgcgga tcatcgcctg gccgggatcc gagcacatgt
     8401 acttgtagtc cacggaacag acaacgcccg gaaaccggct cgcggcctcg gaaaccttgc
     8461 cgcagtcgac gttccgggcg atgttctcgc cgcaccagca gacaaacacg cctattctgg
     8521 ccataagtcc ctaccgcttt gaaacatagt gccggttgat gcccaacgcc tcctcatcca
     8581 ggcccagcgc caggccgacc agatccgaca agtaatagat catcatccca tgctgaaccc
     8641 cgtagcgtcg ctcgacgtcc gcctgcttca tgtccaggtt gacatgacac atcgggcagg
     8701 cgacgacaac gcagttggct ccgtgtcggg ccgcattgct gagaatcttg tgcgacaggt
     8761 tgagcaccgt gtcttcgttg cacatggtca tgccggcgcc gcagcattcc gtcttgtaat
     8821 tccactcgac cggagcggct cccagggatg ccagcagcgt ctccatggag ctcggctgtt
     8881 cgcagtcgtc gaatcgcaga acctccggcg gccgcgtaag caggcagccg tagtaacagg
     8941 ccggcttgta gcccgacagc gacaccttcg ccgcctcttt caccttgtca tagccgatcc
     9001 tttgaaaaac ctggatcaga ttaagaacgc tggtgcggcc ttcaacgggc agttcgacaa
     9061 cctcggacat ctccttccgc aacgccgcat cgcccgagat caacatcttc gccttgagaa
     9121 gttccatcga gcacatcggg cagggggcca gcagatcgtc gtaaccgtcg cgttcggcga
     9181 ttccgaggct tctggccggt aacgccaccg ccagcttctg gttgagcgag tgggcggctg
     9241 tagccccgca gcagatccag tcgtcaagtt cgcgaaggtt cacgcccagc gcgttcaagc
     9301 aggcccgaac cgacctttca tagtcgttcg acgagccgtg aagcgcacat ccggggtaat
     9361 agccaatctc catcggttca acccttgtca ttcttgcccg acgaactcat gcagcggtca
     9421 aaaatccgac gcacaacgtc gcggtcgttc acactttcgc cgatcggatg aaacttgccg
     9481 cgggctatca tcttcggggc cagaagcgaa tccttgaaga ggaaggcaat gctcggatcc
     9541 ttcacaaaag cccctacctt gaacaagccc acaagttcaa gttccttcag ccggccgttg
     9601 cggcggatgt tgctcagaaa cgccttctgg aacaccactg ttcgctgctg cgccggcgag
     9661 gccacattct gttcgaccga gagctgccgc aaggcgtcaa tcacgcccgc aacgttcacc
     9721 gacttgggac atcgagtcga gcacgtttga caagagacgc acagccagat cgactcggcc
     9781 ttcatggcct tgtccagtcg ttccatctgc accagccgca tgatctggct gggaatcacg
     9841 tccatgtggt cggccatggg gcagccggcg gagcatttac cgcactggta acagtcgtgt
     9901 acccgcgtgt gagcgagttc ctcgatgacg tcgaccatgc tcggcgactt ctgcatcacg
     9961 tcgtcactcc cgaaacctgc gagctcaagc ctcgcgaccg tttagccgcc gccgttccgc
    10021 gacggcgacg tctcgctgac ggcgttttcc ccgatcggct gagccgcggt cgctccaaca
    10081 gcatttcaac gaccacgtgc tcggccagcg aggcggccgt gagcacctcc gtccgtccga
    10141 cggcaccgcc cgccggatcc ggctggacca ccaggccgat caccttgccc gcaagctcgg
    10201 gcgggaccgt cacgagttcg cccctgctgt ctcggaccag actgcccggc agtcgtcccg
    10261 tgtccctggc ggccaggaca acaagacggt cgcacctatt tgcctcatcc cggatcacgg
    10321 ccggagtgag accgccttgc ccggagatca tctcgtatgg gatcagtcgc tgccgcagct
    10381 tacggcagat ccatgcaccg agtgcgttgc cctgctgaag cagccccccc gaagtcaaaa
    10441 ggcgaacctc acgtccctcg cggttcgcaa tgcggatccc acggtacgat tccggcttcc
    10501 agtcgcaagt ttccagggtc tcgaccgtcg gccggaaagt cgcgcccagt atatcactaa
    10561 tctcgccgtc tgcaaaaggt tcgatcgcat agtagaatac gcccagacgg cgatatgccg
    10621 cctcccgttg cgcgcccacc gatgcgacaa ccactattct tacctccggc gctgcggatc
    10681 gcaatctcgc ggcgatttca ggaccattct cggccaccga agccgcatct atgacgatta
    10741 tagtgctatc gcgttcccag agtacttgaa tcgcctccgc ccagcccgaa accactttga
    10801 cctcacagcc cagtttcatc agggcgacgc gctgcgccgc cgccgaaacc gggttgctgt
    10861 tcaccaagac cacatgtcgc agtccgcaat gcggcagttc ctccgccaac cgagtcggaa
    10921 aaatccatgc aagccaggac tcgtcacatg aagcctcaag caatgcttca ggacgacaat
    10981 ccaactccgc attcacgtcg acgatcacac ccgtcagcgg tgaggcaacc aatcgcggcg
    11041 ccattccctc ggcttggatg cttgctaggg gtaaaccctg ataaacgatt tcgccggtgc
    11101 tcggaagact caccgatctg aatgtgccca gtggatggac cgggggaaaa acaccgatca
    11161 ccgccagacc ggtatgcgac atctggaacc aggactcatt ccgaaagcag tacttgtcgc
    11221 cccgaattcc ttgcttggag gcaaacaagg ggtcaccgct cgcgagcgac ccggacacag
    11281 tgttccgcct gcgaatcagt cgccacaggg cttgggccat ctcctcggga ctgaccggtt
    11341 ccgtcagaca ttgcgagatg cccgggctgg ccatggagag agccagtccg ccgccgcgag
    11401 aatcggccca gagaatagtc ggtgtctcgg gtttcttctg cgccaggatg cgcagacacc
    11461 tgtctcgatc ctgcttcacc cgggacgttc taaggagaat cccggccaca tcggcgctcg
    11521 ccacaagacg caacgcctct tcggtccgac agaccgtgcg gatttccgcc ccgcaggcct
    11581 gaagcagcgc aagatacccc cgtcccagcg gatccttttc gtccgccaca accaggagcg
    11641 tgcgccggct gtccatgctt tgcctccgtg tcgccgccac aatcgcctct tggtacccga
    11701 ggcaacaagc gtgccaacga ctcctgtttc ggatgccgat gttttcagcg gccgatgccg
    11761 gtacgggctc acctgaggcc tcggatctga cgaaacacga tgaaacgcga gattcaaacg
    11821 cggtatcttg cgacgcaagg acgagcagga cgtcgcggcc ttcaagcgct tgccctggaa
    11881 gctgtaagac cagaccgtac aggtgtccgt cgcgcgtaca cgctttaccc gcccggttct
    11941 gagctcagat tgtgcttgcg catcaaggca tggaagtgag ttctctgcat gccgacatgc
    12001 tctgccgcct tgctgacgtt accctcgcat tgctgaagag cgttgctcag gaagcgacgt
    12061 tccagttcct gcaccgccgc atcgcgaacc tgctgcttga gattcttgaa ctcttcccat
    12121 gtcttcggca gcggagcggt accggccgaa atcggcgctt gtcgaatctc gagaggtaga
    12181 tgcctgacct cgatccggtc ggaatcacag agaatggcca gccgttcgac gacgtttcgc
    12241 agttcccgga cgttgcccgg ccaggaatac gcctccatca tcgccatcgc ctcgggtgta
    12301 aagctcttga agctgatctc attctgacga cagcagcgtt ccaggaaaac catggccagc
    12361 ttcgggatgt ctccctgccg tgctcgcaga ggcggcaggt caatcggcac gacgttgaga
    12421 cggtagtaca gatcctccct gaaagccccg tctttcacca tctgcatcag atcgcgattt
    12481 gtggcggcaa tcagccggat atccacctcc cgttcagagg tgtcgccgac cttgcgaact
    12541 ctgcgcgtct ccagcacccg caacagcttg ccttgcgtct caaggctgag gttggcgacc
    12601 tcgtcgagaa acaaggtgcc cttgtcggcc acctcgaaca aaccctgctt cgtggcaatc
    12661 gcaccggaaa acgacccttt gacgtgaccg aacagctcgc tttccagaag ggtcggagcc
    12721 aacgagctgc agtcgcaacc cagcagcggc tggttcctgc ggcggctgag gcggtggatc
    12781 gctcttacca ccatctcctt gccggtaccg ctttcacccc tcaccaacac cgtgccgtcc
    12841 gtcggtgcaa ccctgcgaat caggccgaat acgcgttcca tggaccggct ttcgccgatg
    12901 atgccttcga accccttatc gatttcgaga gcccggcgaa gcgccgcgtt ttcccggatc
    12961 agggccgatc gctccgcggc ttttctgaac accagacgca gctcgtccgg agtgaacggc
    13021 ttgctgagat agtcggccgc tccctgtttc atggcctcca cggcggactg gaccgtcgag
    13081 tatcccgtga tgatgatcac ctccgccgcg acgccggcgg ccttgacctg cttcaagacg
    13141 tccatgccat gcaaccctgg catcatcagg tcgagaaaaa tcacgtcgta atcgcccgtc
    13201 aaggcggctt ccaggcctcg cctggggtct tcgatgcaat cgacgcggtg tccatccggc
    13261 tccaggcaac ggcggcaact caaaccgatg accacctcat cgtcgatcac cagcacacgc
    13321 tggcttgcga atgtcttctc catgtcagcc atgagcatcg gcctcctgcg ccggctgccg
    13381 gccctcttga tcgttcacgc acagcgttat cgcgaacgta gtctccgttc cggctctgct
    13441 ttgacactcc aacatccctt ggcgcggcaa cacgatcgcg tcactcgggc cgagcccctg
    13501 gtccccgctt tgatcggcta tcttgccggt gagaagcggc ctggcggtcc ggtccgcgcg
    13561 caccgacccg aatcgccggg agcaactcgt cggtggcagg cgatctgaca gccgtgcgcc
    13621 ggctgcccgg atgcaacggc ccttaaagcc ggctgaacgc ggctgtgccg ccgcatcagg
    13681 cgcgttcgat ctgagctggt caacgaccgc gacggacacg gctgacttgc cgcccgccag
    13741 agcggtgtgg ccgcaagtgc cgtatctcaa actggtggac acaccactct cctggcctcg
    13801 cgcgggcctc aagtcggcta cgacaacatc tgcacgaact caatgctgga aacatcaggc
    13861 cagcttggcc atacactgta tgggcgaacc atacacaaaa ccaccccgag actgagaaaa
    13921 acgagccctt caaggcgcgt gattaacgtg caagttcctc tagtgcagaa atcgtgttaa
    13981 taccttgcgg attatcggac cttgtggcca tttgtccgaa ccgagcaagc ttcgggggct
    14041 aatcattcga tctggcatgc agcttgctcg tgaaaaaagg tacgaagtct cgccgcatgc
    14101 ttgggcgggg gctcgggctc ctgcccggtg ctgtgatcgc aaggcccgct tccgtcgcgg
    14161 ccggtgatcg aatcagccaa tgaaaggaga ggtgttgact tggtgacgcc ggacattgat
    14221 gccattctgg caaaacatcg cgacgcgaaa cgcgacgcgc tgatacccat cctccaagac
    14281 gtgcaggaaa cgcaagggta tctctctcgc gaagccgtcg ttcgcattgg cagacatctg
    14341 ggtctgccgg cgagcaagat ttacggcgtc gccacgttct acaaccagtt ccggttccaa
    14401 cctcagggac gcttccacat ccaggtttgt cgcgggacgg cctgccatgt gaaaggatca
    14461 gcggccgtcc tcgatgcgat cagacgcgag ctgaaaatcg agcccggcca gacctcacgc
    14521 gacggcacgt tcagtctgga ggtcgtggct tgcatcggag cgtgtggcct ggccccggtg
    14581 atcaatatca gcggtgagtt ccacgccggc gtcaccactc agaaggtcaa ggggatcatc
    14641 gattcataca gagccagagc ggcaggaagg ggcagcaatg accaagccgc ctgagacaat
    14701 cagcgcatgc tgccacgaac atgccgcgga ccagggctac ccgaccgtcc tcgcacacgt
    14761 gatgagcagc gatcccgctc aacaggatcg gcttgcactg ctcagacacg acagggtgac
    14821 gcgcccgacg atctacgtcg gcacgggaac atgcggtttg ggcgcggggg cccggtttac
    14881 cgtcggggcg atcaaggagt atctgaggca gaacaacatc gaggccgacc tgatcggggt
    14941 ggggtgcatc ggcttgtgtt cggccgagcc gatcgttgat gtccagcttc ccggaaaggc
    15001 acgcgtctca ttcaagggaa tcaccgagga cagagtcccg accctgctgg atgcgatgat
    15061 gcaggggacc gtgcccgccg agtcggtcat cggccagcat cgcaccgagt tcggtgagat
    15121 gtggcccgac gttccgtgtc ttgatgagca cccctttttc gctccgcaga tgcgatgggt
    15181 gcttgccaac tgcgggatca tggatccgtc cagcatcgac gaatacatcg cccgcggcgg
    15241 atataccgcc ctggccagga tgctgtccgc ccaaaccccc gagcaggtct gcgatctcgt
    15301 tgaagcaagc ggtcttcgcg ggcgcggcgg cggaggcttc cccacgggca ggaaatggaa
    15361 attcgcgaga tcggctgccg gctcagggaa atacctggtc tgcaacgccg acgaaggcga
    15421 tcccggcgcg ttcatggatc gcgcggtcat tgaaggcgat ccacaccgcc tgcttgaagg
    15481 catggcgatc gccgcgtacg cgatcggagc cgacaaggct tacgtctaca ttcgggcgga
    15541 atacccgctg gcaattgaaa gactcaaagc ggccatcaaa caggcccgcg attatcgcct
    15601 tctcgggaac aacatcctcg gccgcggctt ctccctggaa atcgtgatca aaatgggagc
    15661 aggcgcgttt gtgtgcggag aggaaacggc cttgatccac agcatcgaag gcaaacgcgg
    15721 gatgccccgc cctcgtcctc cgttcccggc cgttcgcggg cttttcgata agccgacgat
    15781 catcaacaac gttgagaccc tcgccaacct cccgaccctg ctgaccatcg gggcggaacg
    15841 attcagcgcc gtcggcacaa aaagcagcaa gggaaccaag gttttcgctc tctccggcaa
    15901 ggtcgcccgc accggcctgg tcgaagtcgc catgggtacg accatccgac ggatcgtttt
    15961 tgacatcggc ggcggcattc ccaacggcaa ggcatacaag gccgttcaga tcggcggtcc
    16021 ttccggcggt tgcatcccga cccaacatct cgacatcgac gtggactacg agtcgctcaa
    16081 aaccgtcggc gccatgatgg gttcgggcgg cctggtggtc atggacgaag acacctgcat
    16141 ggtcgacgtc gccaagttct tcatggattt catccagcgg gaaagctgcg gcaagtgcat
    16201 tccgtgccgt gaagggaccc gccggatgct cgagacgctc cagcggatca cccgcggccg
    16261 caagagcgag aaggatatcg acgccctcac ccggttccag agcgtcatgc acctgccgag
    16321 gctggctgag gtgatccgcg acaccagcct gtgcggcctg ggacagacgg ctcccaatcc
    16381 ggtgctcagc acgctgcgct ggttcagaga tgagtacgag gcgcacatct acgagcgccg
    16441 ctgcccggcg ggcgtctgcc aggatctggt aacgtaccgt attatcgccg agaaatgcag
    16501 aggctgcacc ttgtgcgcta aacgatgccc ggtgcaggcc gtcatgggaa cgcccaaaag
    16561 cccgcactac atcatcaccg acaagtgcat cgcgtgcgga tcgtgcctgg agagttgcag
    16621 gtttgaagcc gtcgtcaagg aatgaaacgg cagcgcattg agacatcctg cggtggtcga
    16681 gggtcgaagc ggcggtgcaa cagccgcgga aaacgtgcct cgatcaagcg gcgggaaacg
    16741 ggagcctgag aacgccaatg atcaccatcg aagtaaatgg ccgtccagtg gaagccgaac
    16801 caggcgaaat gcttcttgcg gcgctgcgac gtgcaggcat caaggtgccg acgctctgtc
    16861 agatcgaagg gctgtttccc accggcgcat gccggatgtg cgtcgttgaa gtggaagggc
    16921 agcgcggact ggtgccgagc tgcgcctttc cggtttccga aggcctgaag gtacagaccc
    16981 actcgcctcg ggctctgcga gcccgcaaga ccatcgtcga gcttctgctg gccaaccacc
    17041 ccgacgattg cctctactgc gttagaaacg agaactgcga tctccagcgg ttggcggccg
    17101 agctcggcgt tcgcagccgg cggttttccg gcggaaagaa ccggcacgcc ctcgacacgt
    17161 cgagcccgtc attggtccgc gacccggcca aatgcatcct gtgcggcaag tgcgtgcggg
    17221 tctgcgagga aattcagggc gtcgcggcca ttgacttcat cggtcgcggt tcgaaaacca
    17281 ccgtcggcac cgcgttcaac gaaggcgtca acgtatcgag ctgcgtctac tgcggccagt
    17341 gtgtcagagt ctgcccgacg ggtgcgctcc gcgagcagag ccacatgaag gaggtggcgg
    17401 acgcactcaa ccatccggac ctcatggtgg tggttcagca cgctcccgcg atctcagtga
    17461 ccctggggga ggaattcggg atgccgccag gctccgacgt caacggagcc atgaccacgg
    17521 ccctgcgtct cctgggtttc aagcgggtgt tcgacaccag cttctccgcc gacctgacga
    17581 tcatggaaga agcctccgag ctggttcacc gaatcaagaa cggcgggcgt ctcccgatga
    17641 tgacaagctg ctctcccgcc tggatcaagt tcgtcgaaac gttttacccc gatttgatgc
    17701 ccaatctgtc gacctgcaag agtccgcaac agatgctggg ggccgtcatc aagagctact
    17761 ttgccgagcg cgagggcatc gaccccgcca gaatcttcag cgtcagcatc atgccctgca
    17821 ccgccaagaa gttcgaggcc ggacgcccgg aaatgtgccg caacggcctg cccgatatcg
    17881 atgcggtgtt gaccacgcgc gaactggccc gcatgatccg gatgcgggga ctcgacttgc
    17941 gcgagctgcc cggcgatacc gccgatacac ccttcggtga acgcacaacc gcaggaaaac
    18001 tgttcggtgc cagcggcgga gtcatggaag ccgcgatccg caccgcccat tacctgatca
    18061 ccggcaagga gcttgcggac ctcaccgtgc agcccgttcg cggtctggcg ggcgtcaaag
    18121 aggctcgcct gcagattgcc ggcatggaca tcggagtcgc ggtcgtcagc ggcctggcaa
    18181 atgcccgccg actgctcgat cagatcctgg cgggacgcaa ggacctgcac ttcatcgagg
    18241 tcatgagctg tccgggtggt tgtgtggccg gcggcggcca gccgatcaac accgacatgg
    18301 acgccgttcg aaccagaatg caggctctct accggattga ctccgtcgaa agccgtcgga
    18361 cctcgcacgg caacgcgtcc gtgcggcgca tttacgagga gtatctcggt cgaccgctga
    18421 gtgaaaggag tcatcacctg ctgcacacgc actacgttaa acgggaagta ctggcctgac
    18481 gcggccggag agctgtcatg ggcaccgcat tcatcagcac gatcaaagaa cactgccgta
    18541 tgtgctacac gtgtgtgcgc gaatgccccg ccaaggcgat acgcatcgtg gacggccagg
    18601 cccaggtgat cggagaccgc tgcatcgcct gcggcaactg cgtcaaggtg tgttcacagg
    18661 gagccaagga ggtgcgcagc tcgatcaagg aggtcgaggc tcttctggcc tcaggccgaa
    18721 aggtggcggc ctgtgtggca cccagcttcc ccgcggagtt cgtcgacgcc gacccccacc
    18781 aggtggtggg catgctctgg gctttggggt tcgagttggt gaacgaggtg gccttcggcg
    18841 ctgatttggt cgcccgcgag tatcgccgtc tcctgtcccg aaacgacgac aaacgataca
    18901 tcaccacgag ctgcccggcc atcgtcggct acgtcgaacg ctactatccg gatcttctcg
    18961 actctctcac cccggtcgtt tcgcccatga tcgccacggc cagggcgctc cgacaactgc
    19021 acggcaacga cctcaagatc gttttcatcg gcccttgcat tgccaagaag gctgaggccg
    19081 aaagcaagcc tgtggcgggc gagatcgatg ccgtcctgac gttccccgag ttgcgggaaa
    19141 tgctcgccgc gcgcaacatt cgcccggaca acgtggaacc ctgcgaattc gatccgccgc
    19201 acggagcgac cggcgcaatg tttcccgtca gccgcggcgt cctccaggcc gccgacatcc
    19261 gcgaggatct gatgaccggc gaggtcgtcg ccaccgccgg ccgtatgcat accttggaag
    19321 ccatcaagga attcgcctcc ggcgacctgg gagccagact actcgaggtc ctggcctgcg
    19381 agggttgcat catgggggcg ggcatcagca acgacctgcc gctgttcaac cgccgccgcc
    19441 atatgaggaa gtacttctgc aagcggatcg aatcgctcga tctggcacgc tggcacgaag
    19501 atatggaacg ctttgccggg cttgatctgc gccgctccta cgtggccaac gatcagcgca
    19561 ttccgacgcc cgccgacgaa cagctcaacc agatcctcat ccgcatgggc aagttctcgc
    19621 cgaaagacga gctcaattgc ggggcctgcg gctacgacac gtgcatggag cacgccatcg
    19681 cgatctataa agggctggcc gagacggaaa tgtgcctgcc gtacaccatc gagaagctca
    19741 ggcaaaccat caaagaactg gccgcgtcgc acgaacagtt ggccagcacg caggaagccc
    19801 tgatccaggc cgaaaagctc gccagtatgg gccagcttgc cgcgggtatc gcccacgaag
    19861 tgaataaccc cctcggcgtc gtcctgatgt acgctcacct gatgctcgag gagtacaggg
    19921 acaaaaccaa gatgcgcgaa gacctggcga tgatcgcgga acaggccgat cgctgcaaga
    19981 agatcgtcgc tggcctgctc cacttcgcac gccagaacaa ggtggtgcgc tatcccaccg
    20041 atgtccggga actggtgcgg cgatcgatca agaccttcac catcccgccc aacgtcacgg
    20101 tgaagatcga cacggagatc gcggaccccg tcgccgagat cgaccgggat cagatcatcc
    20161 aggtgttgac aaacctgatc agcaacgccg tcgcggccat ggagaagggc ggcacgctga
    20221 ccgtccgggt cacgggcgat gccgacaata tcagattcgt cgtcggcgat accggcgtgg
    20281 gcattcctga acagaatatg aaaaagatct ttgagccgtt cttcaccact aaacagatcg
    20341 gcaagggaac cggtttggga ctggcggtca catacggtat cgtgaagatg cactgcggag
    20401 atatccgcgt ggaatccaac gccgacccgg ccgccggccc gaccgggaca accttcacgg
    20461 tgacgctgcc gagacgcgaa cagagagaga tgaaccaggt ttcggggacc gagatgatac
    20521 ggggcctcgg acaggtcgaa tcagccccaa cggggcagaa ctgagacggc atacgggaac
    20581 atacgaacat ggctgacact aagaaaacaa tccttctggt ggacgatgac gcggacttcc
    20641 tgatgcagga ggaaatcgcc ctgaaagccg gcggctatga cgtcctcacc gcggacagcg
    20701 ggaaacgtgc ggaggaaatc atcgcccgca cccgtcccga cgccgccatc gtggatgtga
    20761 tgatggagga taccgatgcc ggcttcatgc tgtgctacca catcaagaag aaagacccgt
    20821 cgattccggt catcatggtg acttccgtga ccggtgagac cggcctggac ttcgacgccg
    20881 ccaccgacga ggagaagtcc tggatcaagg cggatgcgtt cctggccaaa cccatccgca
    20941 tcgagcagct tgaagccgag ctcacccggc tgttgaagga caagacatga tggatacgct
    21001 gcggattctg gttaccgacg acgaacgggg catgaggctc ggagtcgagc gcacgttgcg
    21061 agacttcagg gtgatggtcc ccgacgtcca cgaagaggtg gccttcgttg ttgaccaggc
    21121 cgaaaccggc gagcaggcca tcgaacaaat ggccacacgg ccgccggata tcctgctcct
    21181 ggatcacaag ctcccgggta tcagcgggct ggaggtcttg gaccgggtgt ccgggacgcg
    21241 cccggagctg ctgacgatca tgatcaccgc ctacgcctcg atcgagacgg cggtcgtcgc
    21301 caccaagaga ggagcctacg atttcctggc caagccgttc acccccgacg agctgaaaag
    21361 caccgtccgc aaggcggcga tccgaatcat gctggcccga caggcccgca aactcgcgga
    21421 ggaaaaaagg caggtccgct tccagttcat ccgcgttttg ggccacgagc tcaaggcgcc
    21481 cctgaacgcc atcgacgggt tcctgcaact cattaagaat cacaccctcg gcggcgacgt
    21541 cggagcctat gatgagatcg tcggccggtg cagcgtccgc gtggaccaca tgcggaagtt
    21601 gatcgtcgat ctgctggata tgacccggat cgagtccggc cagaaatccc gcgacctcgg
    21661 cacggtcgac gtccgcgaga tcgcccggac gtccatcgag acgatcctgc ccaccgccca
    21721 gccgcggaat atcgccatcg agcttcatgc ggacgggccg gtgaccatga ccgccgaccg
    21781 cggcgagatc gagatgatac tgaacaatct gatttccaac gcggtgaaat acaaccgtga
    21841 caacggccgg gtcgacgtcc gagtcgaccg ctccggccag gtcgtgacca tcgcggtgac
    21901 cgacacgggc atcggaatgt ccaaggagga aaccgcaaaa ctcttcggcg agtttgtcag
    21961 aattaagaac gccagaaccc gcagtattct cggcagcggc ctgggattgt cgatcgtcaa
    22021 gaggctggcc atgctctatg acggcagcgc caccgtcgaa agcgaaccgg acgtcggcag
    22081 cacatttacc gtggtcctca aggattcccc cgggccgccc ccgtcggcgg tccctgcggc
    22141 aacaacgatg aacgaggctt gatgtgatga gacaggagac tgtgggctta cccgcttcgg
    22201 atatcatccg tgagagacgg atcgaggaga tcctcgcaca caaccgcgac caggacgcgt
    22261 ccctcgtgcg agagatcctc gccaaggccc ggcaactcaa aggcttgccg atggaggacg
    22321 tcgcggtctt ggccgagatc agcgatcccg aactcctcgc cgaactgttc gccaccgcca
    22381 ggcaggtcaa gcaggacatc tatggcatgc gcctggttct gttcgccccg ctctatgtat
    22441 ccaacttgtg cggcaacaac tgcctctact gcgctttccg ttcggggaac aagtcgatca
    22501 aacgccgggc gctcactcag gacgagatcg cccgcgaaac gaggattctg gtggagcagg
    22561 ggcataagcg ggtgctgctg gttgccggcg agtcttatcc gaacgaaggt tttgattaca
    22621 tcctcagggc gatcaagacc gtctactcgg tccgcagcgg ccggggcgag attcgccgcg
    22681 ttaacgtgaa tattgctccg ctcgaactcg atcagtttcg ccaactcaag gacatgggta
    22741 tcggcaccta tcagcttttc caggaaacat atcaccgcga aacgtacgcc cgcgtccata
    22801 ccggcggccg aaagagagat tacgattggc gggtcacggc catggatcgg gccatggagg
    22861 ccggcatcga tgacgtcggc atcggcgtgc tgttcggatt gttcgattgg cgattcgaga
    22921 tactcgccct cttgcagcac gttcagcacc tcgaagccgc gttcggcgtc ggaccccaca
    22981 ccatcagcgt gccgcgcctg gaaccggcga cgggggcgga tctggccagt cacccccctc
    23041 accccgtatc ggatgtcgat ttccgcaaga tcgtcgccat cctgaggctg gccgtcccct
    23101 acaccgggat tatcatgtcc acgcgcgaga cgccgaacct gcgccgggag acttttgccc
    23161 tgggtgtgtc gcaaatctcg gccggcagcc gaaccaaccc cggcggttac acagaggacg
    23221 aggacatcga tgcctcgcag ttttgcctcg gagaccatcg accgctggat gaggtcatac
    23281 gggacgtcgc gcaactcggg tatattccct cattctgcac ggcctgctat cggctcggcc
    23341 gaacgggcca ggatttcatg gacctggcca agcccggcga gatcaagcat cattgtgacc
    23401 ccaacggact gtcaacgttc ttggagtatc tcttggatta cgcctccccg gccactcgcg
    23461 aggtcggtga gcaacgaatt gcgtccgtac tggctgagat ggacgatcgg gcccggcgaa
    23521 cggcggaaaa gctcatcgag caggtccgcg cggggaaacg cgacgtcttc gtctgagccg
    23581 atgcccgcaa caacacgggg gacccgctat tcatgagcag cgcgtggcgc accgagcatg
    23641 atctgatcgg cagcctgccc gttccggccg atgccttgta cggcattcac accgcccggg
    23701 cactgcggca tttcacgctg gccggccggc ccgtacaccc tcaaatgatc cgggcctacg
    23761 gaaccgtgaa gctcgcctgc gcgatgacca accgggcctt gggatgctgg accgatgacc
    23821 cagccaaggc cgacgccatc gaacgcgcat gccgcgagat gggcgaggga ttgctcgacg
    23881 gcggcgtaca ggttgacctg ctgcaaggcg gagccggcac cagtaccaac atgaacgtca
    23941 atgaagtcct ggccaatcgc gcccttcaaa tcctgggcga accgctcggt gaataccggc
    24001 gagtcagtcc gctggacgac atcaaccttc accaatccac gaatgacact tacccgacgg
    24061 ccctcagact ggccgccatc cgcatgcttg ccgggctgga agaacgcgta gtcggtctac
    24121 aagaagcctt ccaggccgcc gagaagcgct ttgcccacgt ggtcaaggtc ggacgcaccc
    24181 agatgcagga tgcggtgttg atcacgctcg ggcgggaaat ggccgcgtat gccgaagcgt
    24241 tcagccgtga tcgctggcgc atctacaaat gccaggagcg gctgagagtt gtcaatctgg
    24301 gcggcacggc aatcggaacg gggcttgcag cccctcggga tttcatcttt cgagttgcag
    24361 agacgctgcg agacctgacc ggcatcggct ttgcgcgagc cgaaaacctt gtcgatgcca
    24421 cacaaaacgc cgatgtcttc gttgaggtct ccgggattct caaggcttgc gcggcgtcgc
    24481 tcctcaaaat atgcacggac cttcgtttca tgtcttctgg cccggaggcg ggcgtgggtg
    24541 agatccgtct gccacagctt caggcaggct cttcgatcat gcccggcaag gtcaacccgg
    24601 tcatccccga ggccgtcagc caggcggcga tgctggtcat ggggcatgac gcgaccctga
    24661 caatggcttg tgccgccgga aacctggaac tgaatccctt tttgccgctg gtggcctgct
    24721 gcctgctgga gagcatcgaa ctactcgagc gaagctgcga cgcgctgcga aggtattgtg
    24781 tcgaaggtat cgaagccgac gaggcccgtt gccgtcgcca tgtggatgcc gccacggcca
    24841 cggtgaccgc cctgctgccg gctctgggct atgaacaagc gtgccgcatc gcgctcaaag
    24901 cacgccaggg cggacgttgc attcgcgatg tcgttgtcga agaggggttg ttgaccgccc
    24961 aggcattcga tgagatggtc agccccgagg ccgtctgtcg tctcggctcg cccatgatca
    25021 ggcggccgaa cgctgagtat gggcctgtcg gccaaggcgg ggcgccatga ccacttcgac
    25081 acccaaagga tttcggctcc atatcggcat tttcggacgc cgaaacgtcg gcaaatccag
    25141 cctgctcaac gccgttaccc ggcagaacgt gtccatcgtg tccgaattcg ccggaaccac
    25201 gaccgacccg gtcgagaagc ccatggagct gctgccgctc ggtccggtgt tgttcatcga
    25261 caccgccgga gtggacgacg cgggggcctt gggcgaattg cgcgtcggaa aaacgcgcca
    25321 ggtgtttgat cgcacggacc tgggcgtgat cgtcgccgag ccgtcaatct ggagtgattt
    25381 cgaggaacgc atcctcaatg aactggcctc gcgacaaatc ccggtgctcg tggtgttcaa
    25441 caaatgcgac gtggcgatgc cggacgcggc ggttgtcgag cggcttgccg ctcgaaaggt
    25501 gcccgtggtt cggacggcgg caacctccgg ccagggcatc ctcgatttcc gccaggccct
    25561 gctggacaac gctccggccg acttcgtgaa caacccgcgt atcctcgcag acatcgtcgg
    25621 gccgggcgag atggcggtgc tggtcgttcc catcgacaag gaagccccaa agggtcggtt
    25681 gatcctgccg caggttcagg ccattcgcga cctgctggac ggcgatgctt actgcatggt
    25741 ggtcaaggag cgggagctcc gcaatgccct ggaccggctg aaatcgccgc ccaagctcgt
    25801 ggtcaccgat tcgcaggcct ttctcaaggt cgcggcggat acgcccgctg aggtcatgct
    25861 gaccagcttc tcgattctgt ttgcgaggtt caaaggcgac ctgatcaccc aggtcgaagg
    25921 gacgctggcc gtcgactcgc tggttgccgg ggaccggatc ctcgtcgccg aagcatgttc
    25981 gcatcatccc atcggcgagg acatcggtcg ggtgaagatt cctcggtggc tgacgcaata
    26041 cgtcggcggg aagatcgagt ttgtcaccgt tcaagggcac gatttccccg aggacctctc
    26101 gtcctacaag ctgattattc actgcggagc ctgcatgtgg aaccgccgcg aaatgctcag
    26161 ccgaatcatg cgatgccggc aggcccggat acccatcacc aactacggcc tggtcatcgc
    26221 ccacagcctg ggaatactgg agcgggcgtt gcaaccattc ccggccgcac tcgaaatact
    26281 ccgtcgtgcc cgcgatttcg gtgcagccgc atcgcgtcaa gcgcagggcc tgccgtgaac
    26341 cggactcctc ccgccgccgc acccacaccc acacgcgagg agctcttgcg atggcttcgc
    26401 gaggatgacg catcgcggct ggggcaattg tggacgatgg ccgatgaaac gcgccgccga
    26461 aacgtgggtg acgccgtcca cctgcgcggg ttgatcgaaa tatccaatca ctgtgtgcgc
    26521 gagtgtgcct actgtggcct gcgggcgccg aaccgatcct tgcgacgata tcggatgacc
    26581 gccgacgaga tcatggcctg cacggccgcc gcggtcgagt tcggatacgg tacggtcgtg
    26641 ctccaatccg gcgaggacga tggcatcagg caacaatggc tggccgacgt cattcgcaag
    26701 atcaagagcg aaacgagtct agccgtcacg ctcagcctcg gcgagcggcc cgaggaggac
    26761 ctggctgcct ggcgccaggc cggcgccgac cgctacttgc tgcggtttga aacgtccgac
    26821 cccgtgctat acaagctcat tcacccgccg cgccctgggc ggttatccga ccgctttgcc
    26881 attctgaacc agctccggga actgggctat gaagtcggca gcggcgttat gatcggcatc
    26941 cctggacaga cgtacgacag cctcgcggat gatctcgaag ctttccggcg gcttgacctc
    27001 gatatgatcg gcgtgggacc gtacatctct caccccgcga cgcctctggc caaacactca
    27061 ggcctccgcc cgataccccc cgagcaacag gtgccgaaca ccgaattaat gacctacaag
    27121 gtccttgccc tgacgcgtct ggtccgcccc gacgcgaaca tccccagcac caccgccctg
    27181 gcaacgctca acaaggacgc cggacgggaa ctgggcctgt cacgcggggc caacgtggtg
    27241 atgcccaatc tcaccccccc gcaataccgc accctgtacg aaatctaccc gtcgaaagcc
    27301 tgcctttttg agaatgcgga agactgccga tcctgcctca agaaccgcat cctggccatg
    27361 ggacggacca tcggccaagg accgggcagc cggcggcaga ccgcctgatt gactgccccc
    27421 tcgtaagttg tgtcaaccgc ccaatccgct aaactgtgca tggcaaccat caccggagca
    27481 cagaaatgga catgcagcag atcgctcgtt ttttcgaccg tgacaagctg gccaagcacc
    27541 tggacatcga gctggtcgac gtctcgcccg gccaggcgac gactcggatg accatccagg
    27601 acaaacacct caacggagtc aacatcaccc acggcggggc aatcttctca ctcgccgatt
    27661 ttgccttcgc ggtggcctcg aattcccatg gcacgctggc ggtcgcgatc aacgccaaca
    27721 tctccttcct gaaagccacc accagtggcg tgctcaccgc cagagcacgg gaagtcgccc
    27781 gcagcaaacg gctcgccacc tatcagatcg aggtaaccga cgaggaaggc gcgaccgtcg
    27841 ccggctttca gggaaccgtc taccgcaagc aggaaccgct cccgctcgaa gaagacctta
    27901 ctccaccttc atctggcggc ggtcgatgac ccgtttggcc ttgccctgaa agcgctccag
    27961 cgactgcggc ttgaccagct cgacgttcat ccgcagcccg gtaatggcgt ggatctcgcg
    28021 atcgatgcgg tcgcgaaggg cctgcatttc ccgcatacgg tcggaaaaat cctccggccg
    28081 gacctcgaca cgcacggtga cctggtcaag ggcccccgga cgcgacaact cgatctggta
    28141 gtgcggcgaa gtgccctcga cacgcagcag agcctcttcg atctgagagg gaaagacgtt
    28201 gacgccgcga atgatcagca tgtcgtcggt ccgaccgatg acccggctca tccgcaccgt
    28261 cgtccgcccg cagcggcagg gcgtatcgtc cagtcgggcg atgtcgcggg tgcggtagcg
    28321 gatcagcggc aaggcctcct tggtgatggt ggtgaaaacc agttcgccgg cttcgcccgt
    28381 ggccacgggc tccagcgtgt cgggatccac gcactcgacg atgaagtgat cctcctgaat
    28441 gtgcatgccc tgctgaagag tgcactctcc gctgacgccc ggaccgatca cctcgctgag
    28501 cccgtagttg ttgaaggcta cgatgcccag ctcctgttcg atccgggtcc gcatgtcctc
    28561 ggtccacggc tcggcgccga agtgcccgaa cttgagcggt atctgctcgg gggtcatgcc
    28621 ctgatcccgg gccgcctcgg cgatgttgag ggcgtagctc ggcgtgcaga tcagcacctg
    28681 cacgttcaag tcgcagatca gcatgagctg acgaggggtg ttcccggccg acgccggcac
    28741 gatcgccgcc ccgaccttct cgacgccctg gtgcagaccg aaacctccag tgaacaatcc
    28801 gtaaccgaac gcaatctgca cgaagtgctc cggccgaagc ccgcccgcga ccaggaagcg
    28861 ggcgcacaga ttcgaccaga tgtccatgtc ctgggccgtg taggcgacaa acgtcggttt
    28921 gcccgtcgtg ccggatgaac cgtgaatacg gaccagtttt tcacgctcta cggccaggaa
    28981 acccaaggga taataacgtc gaaggtcgtc tttagtggtg aaaggaagac gtcgaagatc
    29041 gtccagcgaa cgaatcgaat cggcgctcac gccggtgctg gcgaatgcct ccctgtagaa
    29101 gggaacgtct gcgacgcgcc tgacgcaatc cttgagccgc tcaagctgca gccgacgaag
    29161 ctcggcccgc ggcatcgtct cggcaggatc ccagatccga ttctccacct tgaactgggc
    29221 catcacactc accatccgct ttcccgggcc ggcggcacaa gcccgacgga aaccccgatc
    29281 gcaggacttc gcccgtcgga ttcgcccgac tagacgttcg cctgttcgta tatttccacg
    29341 ctcgataccg ggttgatacc cttggcttga aaggcctcca ccgccgcgtc catgtcctcg
    29401 aagcggaaca ccaggaccgc gcggttgccc cgccggaacg tgaacgagta cgtgtactcg
    29461 atgttcagcg agaattcctc gatcaccgcg aggatgtcgt ccagcccgcc gggatggtcg
    29521 ggaacctcca gggcgatcac ctcggtgaca ttgaccacgc agccgccctt ctccagcgcc
    29581 gccttggcct tctgccagtc ctgcacgatc atcctgagaa tgccgaactg ctgggtgtcg
    29641 gccaggcaca gcgtcaggat gttcaccccc gcctcggcca ggatgcgaat cgggctccgc
    29701 aactgccgcg gtttattctc aatgaacagt gaaagctggt gaagtttcat ggtccgacct
    29761 ctttctactc gttcctgcga tcgatgactc tcttggcctt gccctggctc cgctcaatgg
    29821 tgtgcggctc caccagacgc aggttgaccc gaattccgat gatatgctcg atcgaactgg
    29881 ccagcttctg cttcaaggcc tccagcgacc gcaccttgtc actgaaccac tcggcgagca
    29941 cctcgacctc gacggtgatc tggtcgagcc ctttctcgcg ggtgagaatg atctggtagt
    30001 gcggcagggt accttccacc gccagcaggg cggcctcgat ctgagacgga tagacgttga
    30061 cgccgcgaat gatgaacata tcgtcgctgc gccgggagat ccggcggatc cgccggatcg
    30121 tgcgcccgca gcggctcggg ccgctgatga acgccgtgat gtcgcgggtg cggtaacgaa
    30181 tgaccggcat ggccttctta ctcagggtcg tcagcaccag ctccccctcc tggccctccg
    30241 gcagaacctc acacgtctcc ggatcgatga tttcaggata gaaatggtcc tcgaagatga
    30301 acaggccctc ctggtacggc gtctcggacg ccacgcccgg accgatgatc tccgaaagac
    30361 cgtagatgtc catcgccttg atgcccgatt ccctctcgat gtactggcgc atcgcctcgc
    30421 tccagggttc cgctccgaaa acaccggctc gcaggggcag ttggcgaata tccacgccca
    30481 gttccttggc ccgctcgatc aggtggatga agtaggtcgg cgtgcagcat atcgccgtca
    30541 cgccgaaatc cttcatcacc atgatctgcc ggtccgtgtt gcccccggag atgggaatga
    30601 cggtggcccc gagggcctcg ccgccgtagt gggcccccag accgcccgtg aacaggccgt
    30661 aaccatacgc gttctgaatg atgtcgcccc ggtgcagtcc gcacgccgcg aaggtgcgca
    30721 ccatgacgct ggcccagacc tccaggtctt cctgagtgta ggccaccacg atcggcttgc
    30781 ccgtcgtccc gctcgacgcg tgtaaccgga cgatttcctg catggggctc gcaaaaagac
    30841 cgaacggata tgtgtcccgc aggtccgtct tgaccatgaa cggcatcttg gcgacgtcgt
    30901 ccagtgaccg gatgtcggcc ggcgtcagcc ctcgctcctg catgcgctgg cggaaaagct
    30961 cgacgtgatc gtaggcccgc cggacgacgg ccgtgagccg acgaaactgc agatctcgca
    31021 actgcggtat cggcagaaag tccggagcgc tggccgggtg aaaaccgccc ggcaaatcat
    31081 tccacatttc cttgtacatt ggtcttctcc cgtgcgataa cgccggtctc agcaggccga
    31141 cgaagcctgt ttctcgcgcc cgaccgatcg accgagcaga aaagccttga tattcgcttc
    31201 gtgcagtttc tccgcaaggc tgcccttcac cgccgataac caggtcgact ccggcagcgg
    31261 caaatacgcg ctcagcacgc cgagcagggc aacgttcagg ctgcgcgggc tgtccagcga
    31321 tgtgaccggc acgtcgtccg ggacgatcat cacccctccc ggcttgagat gccgcttgct
    31381 gccctcaacc tcttcagggg ccatcaccac gacgaaatcg gcctcgcctg ccggaaccat
    31441 cgggctgaaa acctcggctc caaagcgaac gtcgctggtc accgatccgc ctcgctgcgc
    31501 catgccgtgg acctcgctct tcttcacgtc gaggccctcg tcgaaagcca cttgggccag
    31561 gatatccgag gccttgagca ctccctgacc cccgagtccg gcaatcacga cgttggtcac
    31621 accactggcc acgttttcct ccgtcatcta tttgttgcct cccgacgagg ccgcctgttc
    31681 atatgccttg attctggccg tggccagcag acaaggacgt cgcgcgatga tcaacgtcaa
    31741 ctcgtcggcc gccaagctgt cgctcaccag cttctcgaac gccgactcgt ccttggtggc
    31801 atccacgacg tggacattgc gaatccccag cgaccgggcc aggtcctcgt acgagacttt
    31861 ccccgtcggt tcgtgcatga gcgtgcggcc ggtacccgga tgctcctgca ggcccgtcat
    31921 cgccgtcgtg ccgttgtcca gaatcagtat aacgtggccg gtgggaggag gattgtagac
    31981 catctcaacc aggccggtga ttccgctgtg aacgaaggtg ctgtccccga tgacgctgac
    32041 cacccgccgc gcctgctcgg ggggcagtac ctgccgcaaa cccagaccga ccccgatgct
    32101 cgcccccatg cacacgcaac tgtccatcgc ttggtagggg ggcagaacgc ccagcgtgta
    32161 acagccgatg tcgcccgcca cgatgcattt cagcttgctg agcacgccga agaccgagcg
    32221 gtgcgggcag ctcaggcaca gcgccggcgg cttgcctcgc ggcggagccg gctcgggcgt
    32281 ggtgtcaccg gccaggatcc ggcgaacccg ggcaacgttc aattccccga aacgatacat
    32341 ctccggcttg cccgcaacct caatgccgcc ggcccggagc gactcaacca ggtacggatc
    32401 gccttcctcg atgaccaagc agcgatcgac tccggcggcg aacttgcgag ccgtctccat
    32461 cggcagcgga tgggtcattc ccagcttcag cacgctcgct tgcggggccg cctcgcgaac
    32521 gtgaacgtag gagatgcccg aggtgatgat gcccaggccg tgatctccgt cgatgacccg
    32581 atgaagcccc gaggtctcat tccatgccgc aatctgggcg atcttttccc gcagccgccg
    32641 gtgtgcggga cgggcatggg ccggaatcat gacccgacct gcgatatcgt gttcgaacct
    32701 cggcgccggc gccggcatct cgccgaccgg ccgcacgacg gttttggagt ggcatacccg
    32761 cgtggtcatc ctcaggatga ccggaatctt ccacttctca gagatctcca cggcccgcag
    32821 tgtgaaatca aaagcctcct gcgaatccga aggctcgagc atcggcacgg ccgccgccgc
    32881 cgcatacctg cggttatcct gctcgttctg gctcgacgcc atccccggat cgtcggccga
    32941 aaccacgagc agcgccccga ccacccccgt ataggcggcc gtaaacagcg gatctgcggc
    33001 cacgttcaag ccgacgtgct tcatcgtcac cagagctctg gctccgccga aggccgcccc
    33061 cagggctact tccaaggcca ccttctcatt cggcgcccac tgagccttgc cacccatctc
    33121 cccgaaggtc tccaggattt ccgtcgatgg cgtgccaggg tagccgctgc cgagggccac
    33181 acctgcgcgg cgggcggcta aggcaaccgc ttcgttcccg ctcaataaca cacgatcagt
    33241 ctgctgcatt tactgctccg aactggccga aaagatacgg accagccacg agggaggccg
    33301 aaccggcttc ccatcggcgc ccgtaatggc gtgaacagta taaccttctg ccacgccttg
    33361 gccattgtgg gcctgcctta tacgaacgtc gaatcgaacg cttgccttcc ctgacatgcc
    33421 ggccgatacg cacatttcca gctcgtcatc ataacgagcc cggccaagat agcgaacatg
    33481 ggcctccacc accggcaaat atgtgccgtc ggcttccatc tccgcgtagg gtatcccgac
    33541 gctccgcagc cattccgtcc tcccgcattc gaaccattcc agaacgcggg agttgtaaaa
    33601 ggtgcccatc tggtcggtct cactgtagcg tactcgaaac cgaacctgat gcccgtcacc
    33661 tgccacgccg cattcctcgt ccgcgccaag gttcgcacgc ctgtgccgct cggccccttt
    33721 tcgcccgatg gaaccgcgtt cttttagcaa atcgcgcctc cgttggcaac cgccgccaga
    33781 cccgaccgat gagccccgct ccctgaccgc catcgtgcgc ggcggacatc gtataagatc
    33841 gccgtcttgc cggtcgacgc gcggacaatg tgatagaagg gagggtcatt ttgccggtcc
    33901 aatgagaggc gcgaaggggg ccctgaaact cgaggtcgtt cggaaacaac cccctgaaaa
    33961 agacgatggc gattgccggt cagtcccgtt gcgacgctcg gggcagatga tgcaatcccg
    34021 gcctggctgt tcaaaaaaaa cagagcggca ggaacggcgc atctgccgct tctgccgctt
    34081 ctgtttccga gccgtcggga gcgcgcccga cgccacgcct attccagatc acttaccagg
    34141 tgaggttcaa ctccagacgc aagaacgtca gcggatcgtt gcgagcatcc gtgtagtagt
    34201 tgcccgggaa gacaaactcg ggcatgaccc tgaggaacgc atgtttgttg aagacgtact
    34261 tgaagtacgg caccagaatc tgcccgcgga agcagccgct gttgctgaac ccggcccttc
    34321 cagcgtaggt gttctcatcg gcaaacagga actggtagta cagacccagg tccatatcct
    34381 tcgtcggctt gcatcgccag ccggggccga cgcgatgcgt attgcccaga tcccagatgc
    34441 gattctcgat accgtatgag aatccgtagc cttccgtcca tcttggatac cggccccaca
    34501 gaatgtcgaa acgctcgtcc gttccccgag tgctgggatc gtcacctgag gtgaactcgt
    34561 actccaggta gacctggttc ttgtatttgt cattgaaatg ataggtcagg ctgttgagcg
    34621 tgcccaacgc gcagatgtcg cgcccgtcac gctggccgaa ctggccggcc aactggacat
    34681 catatttcca gttgtccgta atgtcaccgg caacgcggcc gccgaaggtg taaatgtctg
    34741 cgttgtcgcc cgaccgacgc ggttcgtcgg tattcgggtt attcctgacg gggataagct
    34801 cggcttcgtt atgcttgtag aagaaatacc cgtcgatctg agtcttggcc agcgacttgt
    34861 tggtcacata taccatcacg ccttgctcat cctgctccgt ggccaaggcc gggacgcgct
    34921 tgttggcgat ccgcggaaac cagtggtccc actgggcgtc cacttcgaac cacgcgacgt
    34981 cgaattttgt cttgatgtct tcggcatcgt attcgaagcg gaccccgtcc acgaagaacg
    35041 tcctggatcc atccagcgga gtcccatcac ccgtaagcca cgcgaacggc aggtcctgcc
    35101 ggcccgcggt gatggtcagc ggcagtccga acgccttctt gtacttcaaa tacatgttgt
    35161 caaacagaac ctggtcgcgg gcctgttccc agtcgctgat cgtcggatgc tcgcaccagc
    35221 ttcggccttc ccaagtaagc ttgaggtgga actcgagatc ctcgaagttg aggggtttca
    35281 ggggctgcaa cgtgccccac catctgccgc ggaagcgcag gtaggacgtc tcatggcccg
    35341 tggcatgctt gtcgaacccg gtgttgttga ggtactccca acgattgcga aggtccatgc
    35401 cccagttgaa acccgggaaa ggattcttcg tcttctcgac aaattcatca aaaggatctt
    35461 ctgctgctgc ggccggctgg gtggccggct gggccgtcgc catcctggcc gtacaggcga
    35521 ccagcagcgc caccagaaac acagaggtca cgactgtcca cttgcttctc ataggttttt
    35581 gcgctcctgt gctagaattt ctccgtgctt gatccgtcgt tcctgagccg tctccgtcac
    35641 gacggcgtta ttcggctacc gttccgattg tgcgacaaac ccccgcgttt tctgatcgac
    35701 tgaccgccct aatcgaccaa cgccgggatc gaagccgcac cgcttcgata cgaacctgaa
    35761 tactttcagg ttacatcggc aattgaatgg tgcctatcgt tagaagattt catggtttct
    35821 gtcaagtagt ccctgcatca tggcgcgaaa aaatcgccca gacggaatac cgacgacctt
    35881 ccggacgcta tcgtgtctga ccaagtctgt tgacgcacat ccgtctctcg gagcacttgc
    35941 cgggaaagag actggtcctt tcttgctccc tttcttgggt tccgcagcca tgccaagcca
    36001 gcagacgcct acgcacctcg gccatttcgc cgtatcatga ggaccaacct gacactctat
    36061 ggatctgaaa acatccacaa ccattcggct cacctgtgcc cccggattgg cacccctgct
    36121 ggaaaccgag gttcgccaac tgggctggcc gatcacatcg gtggacgacg ccggaatcaa
    36181 caccaccggc accctgatcg acgccatgcg tctgaacctg catctgcgaa ccgcgttcta
    36241 cgtgct
//
LOCUS       DAOTPW010000017        36051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1271_length_36051_cov_5.981331, whole genome shotgun sequence.
ACCESSION   DAOTPW010000017 DAOTPW010000000
VERSION     DAOTPW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 36051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36051
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1271_length_36051_cov_5.981331"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..643)
                     /locus_tag="P5316_02700"
     CDS             complement(71..643)
                     /locus_tag="P5316_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRR83894.1"
                     /translation="MRVLSVNGSPRPKGNTHLMLQRVCGHLSKAGHEIDDVCLEEMGI
                     APCRACMVCAEVKDGRCHGHDDACNGLIDRSRAADVILLGSPVYFGSVTGQIKAYMDR
                     VGFVSRVGDQFLARKIGAAVVPARRAGELFTFAQLNMWFLINGMIVPGSSYWNVGHGL
                     REGEIAGDQEAMATLDELAKNILWLLEKLQ"
     gene            complement(760..1599)
                     /locus_tag="P5316_02705"
     CDS             complement(760..1599)
                     /locus_tag="P5316_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83895.1"
                     /translation="MVIDEPQNPFVYPPEWVGLTRDLAGSRALSVAGYEVEVHITVEQ
                     LDLVYLPLLAMLVQHSGDDEERVLAGLGGIPGSGKSTFAAAAALVAGRIWPSGRLAVV
                     GMDGWHYPNVVLDARTVLDERGHPVPLRSRKGGPQSFNVPAMADALESLRPARRSINL
                     PAYDRRLHDPVPGAIVIDARTQIVLVEGNFVLSTTPPWDKVSSLLRPRLFLEADLAVA
                     RERIIKRHIRGGASPEQAREKFETNDLINIAAVEETEANADFVVRLDPNPQIRVQAAR
                     GRR"
     gene            complement(1613..3076)
                     /gene="purB"
                     /locus_tag="P5316_02710"
     CDS             complement(1613..3076)
                     /gene="purB"
                     /locus_tag="P5316_02710"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRR83896.1"
                     /translation="MNSNKDTYENPLVSRNASPEMCRLFSPRHRILTWRRIWLAVAEA
                     QAQLGLPVSRRQIAELRRTMEDIDWDRAAEHEKKLRHDVMAHLYAWGDVAPKARGILH
                     LGMTSMDVVDNADLLIMREAMVLIRDWLVNAIDALGAFARKWRNLPCLGFTHYQPAQL
                     TTVGRRACLWAYDFVRDLEELEDRLGRLRFRGIRGATGTQASFLSLFDGNAAKVVRLE
                     KIVTRKLGFEDVEPVTGQTYSRKIDAQVVATLAGIAASAHKFANDIRLLAGMKEIEEP
                     FEKSQVGSSAMAYKRNPMRCERMTGLARYVISVMSSPLQTAAEQWLERTLDDSSNKRL
                     SIPESFLAVDGILRLVTDVSRGLVVYPKVIGAHVQAELPFMATEEILMAATAGGQGAR
                     GKRARPGDRQALHELIRRHSQAAALEVKMHGRPNDLLQRLQKDPAFANVDFRKALDAR
                     RFVGLAPQQVDHFVKTVIGPILRRHSSARNKQVTLNV"
     gene            3427..3900
                     /locus_tag="P5316_02715"
     CDS             3427..3900
                     /locus_tag="P5316_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83897.1"
                     /translation="MRKGRLAPSRLFPELALFETDQERRDVIRRYWREKVGRRQRRFY
                     LAALLFGSAVAMGLTILALKRSLAFWGLSGEGWVGGVIAGVYPFAVAIGSQYLVRKPL
                     IRYLREQLAAKGVPICIECGYDLRGQVDPRCPECGTAFDPKLIKRPSDPAAGPSD"
     gene            4621..5160
                     /locus_tag="P5316_02720"
     CDS             4621..5160
                     /locus_tag="P5316_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR83898.1"
                     /translation="MYFVTFRLADSLPAEVMKRLRAKRDAWLASHPPPLTEEQIQEYR
                     RIWTARIEKLLDSGHGECVLRDPACRKPVEATLRHDDGKAYLLGRFAIMPNHVHALLQ
                     LLADSDLSNTLQAWKSISARQINTYLRRSGSLWQDESFDHIVRDDAAMNRLTKYIEDN
                     PRCLPVGSYTVGTGLLQAQ"
     gene            complement(5865..7088)
                     /locus_tag="P5316_02725"
     CDS             complement(5865..7088)
                     /locus_tag="P5316_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005768977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR83899.1"
                     /translation="MYYPRAIDDAWRKAAEQFPVLLLTGPRQVGKTTFLQHTAERGRR
                     YVTLDDPGIRALAVQDPALFLERFEPPVLIDEIQYAPQLLPYIKMAADSRRKTGLFWL
                     TGSQQFHVMKGVSETLAGRVAIVNLLGFSSRERHRLRLDVAPFLPTRQRLKEREGSHA
                     SSSLKHVYRDIWLGGFPALTAGPVKDRDLFYSSYLQTYLQRDVKDLTQVGDEAAFLRF
                     LKACAARTGNLLNLSDLARDADISVNTAKSWLSILRGSMQVFLLSPYHTNVTKRLVKA
                     PKLYFLDTGLCSYLTDWSSPETLESGAMSGAVLETYVVSEVLKSWWHRGQSPEVYYYR
                     DKDRKEIDLIFLQNRTLYPLEVKKSASPGPDWAAFFNPLGKLKMKVGEAGVVCLSKDL
                     LPLGENVNAIPVGWL"
     gene            complement(7821..9881)
                     /locus_tag="P5316_02730"
     CDS             complement(7821..9881)
                     /locus_tag="P5316_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR83900.1"
                     /translation="MCNTLLFSLVMILATQTQSAAELYVAANGNDAWSGGLAAPNEQK
                     TDGPFATLERARDEIRKMKKKPGESPAGVTVWIRGGDYHLDKTFELVAEDSGSEKMPI
                     AYRAYKDEKVRLLGGKRVSDFRPVTDRAILDRLEPAARGKVMQADLKAAGVTDFGDPV
                     AAAKRIELFFGGKPMQLSRWPNEGFTKIADVVGGKPIKSHGKEGDSVGKFTYEGDRPK
                     RWIGESDIWLHGYWFWDWADAYQKVESIDTEKRIINLATPYHHYGYRKEQRYYAINLL
                     SEIDSPGEWYVDRRSGTLYFWPPAGLATNETYVSVLGTAVRMENVTDVTFRGLVFEYV
                     RGTAITIKGGKRDLVAGCTLRNIGGQAVVVNGGEKHGVAGCDIYDTGDGGISLSGGDR
                     AKLIPGGHFATNNHIYRYSRNSNTYRTAVSLAGVGCRAANNLIHDAPHMALGLSGNEH
                     VIELNEVHHVVMETDDAGAFYMGRDWTWRGNVIRHNYFHHIGEFKSWVGTQAIYLDDW
                     ASDVKVHGNICYKVFRAVLVGGGRDNTVENNILVDCNIGIHVDSRGLGWAKYYFDRTD
                     NTLVERLNAVPYKEPPWSTRYPQLLTLYQDEPALAKYNELVRNVMVGCGKAIDLHDGL
                     TDKVVAMQDNSIDEEVYLKDGEANRFQLKPDSPMWAKGFQRIPVEKIGLQKDEYRSAL
                     PEAQ"
     gene            complement(10135..12069)
                     /locus_tag="P5316_02735"
     CDS             complement(10135..12069)
                     /locus_tag="P5316_02735"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRR83901.1"
                     /translation="MSNPLESRVIELGKHVVRMTTAAGSGHPSSALSLAHIVAALMYE
                     QMRYDPADPWNPASDRLVLSEGHSVPIVYAAYADLGGVVGKSPAAKRALTLDDLKSLR
                     ELDSVLDGHPNPAEGFPFFDAATGSLGQGLSVGAGLALGARLSKIDKRIFVIIGDGES
                     REGQIWEALDFIIDHELVNVCPIFNCNGQGQADYVSKQQSADVLVGKLKAFGYDVQDI
                     DGHKMDQIASAVAKVGKGSVPVAIVARTQKGWGVDSLKDKSNHGKPLTAAQVAEAEAS
                     LDKVAAELKLPKVCGPAPKPPMPPSANGPAKRDKIAVMPFAEAVAKTGMIEAVAKNKL
                     STRRAYGAALFALGHADERIVALDGDVSNSTFANVFAKAFPNRFFECKIAEQNMISTA
                     AGLSAAGYIPFASSFAKFLARGYDQFEMAAITRANVKLVGSHAGCSLGADGPSQMSLP
                     DMAYFRAYTTAEDGRGNPALTLFHPADAVAAYRLTEMAANINGLCYVRTHRPDVPLIY
                     PQDAVFKLGGCGKLAEGDSVTIVASGYMTTLALKAVRELEKAGIKCNLFDAYCLPIDA
                     EPVLTAARKANGRILTVEDNFAGGFNAALAEAAARGGDVKVFAMTCPRIPKSGKDGDI
                     VLAALGLGVQNIVARVKELR"
     gene            12296..14419
                     /locus_tag="P5316_02740"
     CDS             12296..14419
                     /locus_tag="P5316_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRR83902.1"
                     /translation="MNCKWLVGAIGLLGTVLALAAHAAEKVPLPESSYALPNNFYGAH
                     LLVGDGADGTKGDKHLRWARFLVGRWGYAKTLLADIDKNTKQPPQGWIDYVNKCYQLE
                     LIPVLRLGGHYGGGGWRKPEADSPGDYTSIAQAVKRVVAGLPRTDMCPLYIEVWNEPN
                     LAVEWSGKANPQEYADFFVAVAKAIRSIGDPRIKILNGGLATSPEWAEALCKANTEFI
                     SSFDLWSCHPYPMNRPPHLNHHNKNIKPDSELSIDSYLLELAKLRECGRDNVKIMITE
                     TGWDLGNAVYTKSEGHPIIDEYNRADYAMRAFRDYYPNWPELVAVFPFEFSNEGWQQF
                     DYVYPESGINPDGSPTKPHYQYTVVAALAKPTDTTGAISGTVTVAGLDVRLDGAEVSI
                     GGKPFTSDPMGNYFFGRLEPGSYSLSISKPGFKALKQKVQVAAAKNSVVDVALEATLK
                     TTLSGVVRSGDDDKPVAGAVITLQPGKHEARTDSDGRYILENVIPARYKLSATAQGRY
                     KYEAADVRISSEQENRHDLRLGQLAKLPPGENMLNNASMEAGGGGGGKEWIALGFETL
                     KVDGDVMRSNPGGVADKTAHTGRLSQSMRVSPKETVIRQITHYNTARVGTKYFAGIWI
                     KTDCPDDQGAAWITFDATDNSGGVVGRLGPSRKVTGRSEWTWVSLEGPAPKGSERLSL
                     NLHTQGKSGVAYFDDAFVGVVTPAR"
     gene            14424..15281
                     /locus_tag="P5316_02745"
     CDS             14424..15281
                     /locus_tag="P5316_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR83903.1"
                     /translation="MRLFVTGAAGRLGLRVLERFSPSHDIVAFDRADPPRSLPASVRY
                     VQADLRDARAVREACAGCQAVVHLAAIPSRMPTIPQSEIFAINTQGTYHVLEAADRAG
                     AGMVVLAGSLCAIGLPTSLENHGLSYLPIDEDHPCRPNHAYDLSKRLNELTAETFSRL
                     SGVPTICLRFPALIDVRHDKWFAREIHSSSPRLVLGDYLDFSDAVGAIETALRRRDLK
                     HELLFVNAETIGTATPTAQYVQRFNPRVEWRGPAPNATTPLINCARLKRVLNFTPAVT
                     WQQDMAVSG"
     gene            15296..16744
                     /locus_tag="P5316_02750"
     CDS             15296..16744
                     /locus_tag="P5316_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83904.1"
                     /translation="MFTCVGRVEYPTTASRPAGGNAPLLALMILPTTLLAGLTPCLAD
                     TASQPATPSQDVARFVVPVRWERFASPNLRNKDVQTAARMLLNSVRYNLAWAGVALKE
                     APEGDCYLITDMNEPGIRPPASAACGIATAIAAGIYDEKTCGMLKGQALSRTLKIIRD
                     VAAVHRVNGDPAKGWGDHWQSALWAMLAGRAGWMLWEHLDPPTRHMTAAMIEYEADRF
                     IRPGYTVPYWNGKGGDSKAEENAWNATVLQLAIAMMPRHPRVPQWKEIASELMISAFA
                     REEDMKSNTTMLDGRPVKEWLKGYNIRDDGALVNHNIIHCDYMTTVTLNLHALLLCSL
                     AGQPAPQSADFNAALVYRSLIEQNWPSPPYRPPGGTMYVPGKAEVYYPEGTDWSDYRF
                     DIFYWIDSYAHLFGWDKGLSHQASDWMRLRAGRILEMQGRHPDGRMFAKGEFETYGGA
                     EQMTCWVMADAFLLHWLHDAQTLSRQADWNQP"
     gene            17138..23224
                     /locus_tag="P5316_02755"
     CDS             17138..23224
                     /locus_tag="P5316_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83905.1"
                     /translation="MEFLCILLSVLLFVTLIGHGLWVAIAWLLRGGHPKSDRRSFEPT
                     LDDDRAATARYLTHLQARGLIDAETHARLAKLMAEDVHSVRAYLDKKHTPAKTTQPTA
                     EPWQRPAGIQQQPAAPVASSPAESLPWAGATESAPVPALAGKPLDTGSGQKPPVAPGG
                     TIGAAAPPPVTSLPTEPPSPAEPRRPFGEVVAAFMAEKNIRWGELVGGLLILCCSTAL
                     VISLWAQIEAIPILKFVIFTTVTAALFGVGLFVQHRWKLPTTGHVVLIISSLLVPLNL
                     LAFAVFSPRGPTINAWTLIVELPAVALFAWLTLLAGRVIMSDGARLFASGIVVLSVCS
                     LVIRFLSPLSASGLVAVGLLPVGCYVIVMGISLRRQSRVACIEDTDAKRLILQLGVQT
                     FACLVPLGLLLGESGHLAGALHVLSPLICALAAPALLAGLLLVSRLAPAVPAQTRLTA
                     TSIALVAAAVMLIGVGLAWPTPSRLLPAMLLNALAAAAVCSSIRHPAMHAAVATWLTL
                     AWILGIHMICGAVSWSEVGSRAMLAALFSARTGQALVGPVAACAVAAALLHRRQRREL
                     AIGYDLSALVFSLLSIGVVTWFGFGVPGDAQHVIWVYLVYGAAAMVGAGRLKSTRVAW
                     VGCILIQMAIIQAMVYAWPLQQFPWPTALLIGAGACTVAAVVLRLARLTSDAANTYVD
                     SLAGFATVGSWLAVVWMLNTLPSHSLAAFSARMAWTSVIWAVVAIVKLWPILLTGAEI
                     AIIVSTCTALQCHLRSLEWYRLLSSPFHDPWVWQAHLMVIGGFGLVLIVARQAITQWF
                     APLTSGDTPEAPAPSDAGWRSVARQLLEPWFPATDRWLVALPFLALVFLAGWSVAPAV
                     VVEHGAAASKVAWGLHAHAAGAGSWILLALVAATLLLHIWEGLRRWALLGIIAAAACG
                     LLLVVARFESRHQVVDAWRWLSACAFLAVSVAFWMRGYWVDRLLQMARCRPARQSGNT
                     DITRVCLFCLFALPAVLLTASHMAAAGSMKLPISPDLTELRLHVALLGPVVLVIVSVI
                     GYGLSERQVGYAVAAAGLACAAVTVTELHILRQTKQPFSAGFLIWLTQLNLIVSSVVP
                     IFWKVLRDLFRTKDDRSDYPSGPLIAGRVAVGILLAVAATGMWISPQFLPSVTVAAGT
                     LWGIAAALLVEYALLTSCRPSSANQRGHRESVWVLYGVALIACALAPLDTGNWLCFHV
                     AMVGFVTAAAIRLWAGDRQARELVGAGWQETFDTASADVSGAVRQIDHDLSCTRCGYN
                     LRGLSPSSRCPECGLEIPGSVETAVSRLTPQSTAERAQARVQAVTGVLACIFLATLLA
                     MRAATDDPQRPWWSTGTMCALSVLCIITAAWAPRRALAYLGGVEICLAASVWWVRFFW
                     LRLATFSGANLIDLVNVNVIALAAAGITWLLVERRFVLPRVPSGHTERWPSFHHAVAT
                     VSVVIVFLLALRAICLAAIGEPAGNSIAVSWLAWTAAVAIVLVCGLDSKSVHLPAGIY
                     LLGLTGMSQIMAQTGMTPRYLEWAMSVGLAGYAVVTTLSWRRWTRTGPLAAARQTTDG
                     WLPSANSILALIAMLLALHVSWGHPALVWRMLIVISPLLCAAWAVLTAVEARRLVMQT
                     CAMQLLAAAAILFAWSWVSPEMAAGVLHRAVGLIAAVALATPVLAVVRTRIAAGDTWP
                     TALTRSIITANALAVAGLAYCCASDALSILGDDLLPLTRPAVVAMVLALPLMIIYCIL
                     FATRDRLDPLQLDARRKEAYVYAAEVLAGLLTLHVRASMPWLFTGVIEQYWPLLVIAL
                     AFAAVAGQEACERYGQHVLARPLGRTGVFLPALSMLDLFIAASRVHFSVVLFTTGVLY
                     IVLAATRRSMVMAVLAASSFTGSLWYLLYRTPGMGLTEHPQLWFIPPAIAVLIATHLN
                     RDRLDEMQRRTVHYACLLTIYLSSTADIFLIGVAQAPWLPLILALLSVVGIFVGVALR
                     IRSFLVLGTGFLCLSLLTMIYHAGSNLGWVWIWWASGIALGIAILFVFALFEKKRNEM
                     VALLDQVRKWHD"
     gene            complement(23353..23781)
                     /locus_tag="P5316_02760"
     CDS             complement(23353..23781)
                     /locus_tag="P5316_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRR83906.1"
                     /translation="MSMLVDTTIWSLALRRRTSELNRRERLLVQEWASLVEAGRILLI
                     GPIRQEILSGIRRESDFLAVRTRLSAFGCIEILPEDYDQAAAFFNVCRGKGFAGTAID
                     LLICAVAARADVPIFTTDEDFARYAKFLPIRLHKLPEIPD"
     gene            complement(23778..23984)
                     /locus_tag="P5316_02765"
     CDS             complement(23778..23984)
                     /locus_tag="P5316_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRR83907.1"
                     /translation="MPTNLALDDRLIEEARKAGKHKTKKDAVTAALKEYVQHRKQREI
                     LTLAGRVEYWDDYDHKALRRGRRQ"
     gene            complement(24135..24689)
                     /locus_tag="P5316_02770"
     CDS             complement(24135..24689)
                     /locus_tag="P5316_02770"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRR83908.1"
                     /translation="MNTIAGFLEDHPDLERLISDTFGGTSDARLGADVIAYRLIAACA
                     IGFLIGHVYRRTYTGQRFAPTLPDTHLLLCLGGALIWLVVGDNLVRAFGLAGTIGLIR
                     YRTIVRDPKDTTILLFSMIMGMACGLGQVLVAVIGTVVVLAVMCLLYLGHRRSLTVAN
                     RKAADLLSLLDGGQTDGKKPPQQK"
     gene            complement(24686..25432)
                     /locus_tag="P5316_02775"
     CDS             complement(24686..25432)
                     /locus_tag="P5316_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRR83909.1"
                     /translation="MFEIEQAPISTTSQRVERKYALPDAMLDLAVARLSELLPIHRYA
                     GDHDWSSIRTTYLDTADFAAYREYLVRLPLRKKIRIRQYGVAGEFSNLCWVEIKIKNH
                     RLSLKRRFCCRKPELAALMRGEDVLDRVAPCNEGDITQIYSAIRAMILEQRLRPAVRV
                     EYERLAFQGPDSRSFRITLDRNLRFVSGCGCYSGILEGLVIESKHHGDEPEHLREMRR
                     SLGLKRVKRFSKFARSMQRILELQSQRCIS"
     gene            complement(25432..26277)
                     /locus_tag="P5316_02780"
     CDS             complement(25432..26277)
                     /locus_tag="P5316_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HRR83910.1"
                     /translation="MSSCRFHKRAAFTLIEVMVTIGVIALLLSILLPSLSRSRENARS
                     VNCRSNLHQWGLAILMYVNSTNGVLPFEERPDPRPGMGEDENGDKVWDDVPASAGRPA
                     QVRGWICWFDCLDRYMGKGVSAEQVKICPTVQRFEPNREESYRMNSKLADYSLYKKDG
                     TRNDYFMPYRRVETLKRPSDTVVLFDGDVGTGPVDLGPGSPPPPSFKGRWRLHNDDVN
                     YRHNIATNLLFADWHVENMKKKALALRSYNKADYDAGRPNPVGSIIWQPPDMGPWDPD
                     PGLGE"
     gene            complement(26511..27389)
                     /locus_tag="P5316_02785"
     CDS             complement(26511..27389)
                     /locus_tag="P5316_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRR83911.1"
                     /translation="MRTRTGGFRIGFRRGWSDWQKDLPALATWAKDAGFEAIDLAQAR
                     PEDIGQVRDAGLQVVSVDMLDWSALLSPDAGKRRSAIARNAAYFKEMATLGVQVFFTV
                     AIPEDPSRPGKDNFDLAVASYGDLAPVAESLGATIALEGWPGSPPFANLCCNPETCRA
                     MFREVPCRGLGINYDPSHLIRMGIDHARFAAEFADRIIHLHAKDTEIITDNLYEIGLY
                     QQSIFQKPFFCGEFVWRYTIPGHGVARWSHILAILAKSGFGGVVSVELEDAEYNGTEL
                     GEQTGLLASLQYLETV"
     gene            27674..28582
                     /locus_tag="P5316_02790"
     CDS             27674..28582
                     /locus_tag="P5316_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83912.1"
                     /translation="MPDTIRVGMIGCDTSHCGAFTKLFHEQAPAEGVPAVRVVACYPS
                     FSPDVKSSADRVEEYKKNLVEKWQVKMTASIEEMLKEVDVVMIESVDGRRHLKELRPV
                     AEAGKPVYIDKPFAASLGDAKEMFKIIKDAKLPAFSCSSLRFDSRVAGFADLTDKHGK
                     VVGCDAFGPAHLEPTNPGFFWYGIHGVEILYTIMGRGCRAVQCMSTADGDLAIGAWKD
                     GRLGSMRGIRKGKAEYGAMVLCENSLQPLHPVGDYYPGLVKELIKFFNTKQPPFPIED
                     TLEICAFIDAALRSDKKEGDEVALDL"
     gene            28746..29597
                     /locus_tag="P5316_02795"
     CDS             28746..29597
                     /locus_tag="P5316_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRR83913.1"
                     /translation="MANQNTSHRLLLGHSPDPDDAFMFYGLATGRVDSGRWRFEHILQ
                     DIQTLNERAMRGELHITAISIHAYPHVAGQYALTNCGSSMGDGYGPMIVARRPMDVAA
                     LKGKRIAVPGRMTTAFLALNLLLGKDSFQHEVVMFDQILAHVADGKADAGLIIHEGQL
                     TYRNNKLHCVVDLGVWWKELTGLPLPLGGNAIRRDLGEGNMREIAGIIKNSIQYSLDH
                     RDEAVQHAMQYARDMNQSLADRFVGMYVNEWTLDYGDTGRKAVRELLRRGHAAGLVPD
                     AGRIDFV"
     gene            29701..31872
                     /locus_tag="P5316_02800"
     CDS             29701..31872
                     /locus_tag="P5316_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRR83914.1"
                     /translation="MLCKHDRQVRLQGMRPASGNAAKVSIGIALLSSVLLTADQSAFP
                     ADDGLLAWWRFDDGKGRTAVDAVGQTPDQLIGNVKFAEGVSGTGLKCDGFTTCLTRRA
                     KDAPKPGDAFSVEAWIAVQAYPWGWCPVVSQRTGHTAGYLFGVDARGRVGLHMAVGGQ
                     WRECTSEAMVPLMTWVHVAGTFDAGSGLTVYIDGAKAGTLVSSGSPTYADDVDLLIGR
                     NHTKEPAMFSALWGAADQPVHFAFDGIIDEVKMHGRCLTADEVRKAFQAVRPSAPLDL
                     KPRRMPSGPKDIHRFGAFYHHLKYCEEWDAIWRGNGPDVVVAFKFAPVRLVFWRGVSY
                     APCWVTEKDNWFTNEFMERGPDRKTRGCSESMSDKRAHYSHVKILENSDARAVVYWRN
                     APVGINYEFAFVDEITGWGDWSEEYHTIYPDGVAVRKVVMWSSNFKEWHEWCQSIQPL
                     HAGQRPEDVLDAKRIMSVANMNGETKTFGRESGEKGYGFPTIPGANIQIVYLASKYNP
                     FLVLDDREGKNDRGRPGPEIVRASGDGWSEHSVFPWGNHWPVTQIPVLARYPQAADRP
                     SHTWTSTQYSAAYETTDNSMTKIMLCGLTDNSVEELLPLAKSWLRPPKLTVNSAAFVN
                     EGYDPAERAYVLACKDHGKPAKLEADIAANEESPLVNAALVIRDWGKAGATARLNQVA
                     VEAGEDFAVGHRDRMEGTDLIVWIRTTSTTPVHIELTPAGE"
     gene            complement(32019..32450)
                     /locus_tag="P5316_02805"
     CDS             complement(32019..32450)
                     /locus_tag="P5316_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRR83915.1"
                     /translation="MSARSDAVLHEVWCLNMAIRTKRWDDPREPEDGYRVLITRYRPR
                     ALPKADETWEAWLPNLAPSAELHAAFYGKGRPPIGWQQYRARYLREMKEQISTITELA
                     KRAANGETITLLCSSACILESRCHRSLLKALIEEHMAIPCS"
     gene            32587..33405
                     /locus_tag="P5316_02810"
     CDS             32587..33405
                     /locus_tag="P5316_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83916.1"
                     /translation="MVIRLLITVKTYPIPSAKYDELVCTAGVTENGDFIRLYPINFRD
                     LPYTQQYRKYQWIEVDADKHTGRDVRKESYRPRPDTLKVIGKPIPTRNGDWSERAKYV
                     LKKKAQSMEELQARQEADQTSLGIFRPKKVNDLIIAPDDREWKPAFQAAMRQARIWDE
                     RTVSKEPPRKVPFKFRYRFECDDPRCRRNHEMMIEDWEVGALFWNLRDKGATEAEAAQ
                     GVRSKFLDGLCAPDKDTHFYVGTVLSHPKSWIVIGVFYPKLEKTRVPDLPTLFD"
     gene            complement(33498..35837)
                     /locus_tag="P5316_02815"
     CDS             complement(33498..35837)
                     /locus_tag="P5316_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83917.1"
                     /translation="MVYLVAAADSSWQKFAVRYDGNIFISRSCTKAFGAEAKRLKWPG
                     DSIHFWIGTGATPYFREDHKGSMAVAEENLPIMINRWERKGLQYEQESFATLLEGPLD
                     PNDPARDEQTPAVLMIKLTARNSGREPRASQLWFQTRPDSPFEISGKRIIAKLGDDPT
                     KTERVLRAIISCPSGTETKLERVEKLPSEAVNAVACRFEVTAGGSQVFYLAIPFVSDV
                     AGADADKLEALSYDAERRRVADYWRSMIQRTTRFSTPEPKFNYLSRFVVPHIHISTTK
                     DPKSGLYMVPAASYCYRVYANEACFQAMLLDALGDTKRAGQYLDTFVALQGSRMFRGA
                     YTPSNDGVYHGAKVNKEYDYTAHEYNLDHGTVLWSLARHYKYTRDKAWLEKTLPSMFK
                     AVEWIERQRKATMHEDIHGRRPIEYGLLPPGHLEDNADWGYWFSVNAYCVAGMTEMAR
                     AMADIGHPDAEKIARQAAGYCEDLRSSVIRTAEISPVVKMRDGTYSPYIATRARQRFR
                     QFGPLQAEFYSRYGKPEDVLPCFRLSGTREVLYGPIILLDLGMFEPNDVVADWILDDW
                     EDNLTLSGERRFNVHGITDERLWFSQGGMVWQSNLQNPIQMYLKRNEVPAAIRNIYNN
                     FVACLYPDVNTLTEEYRMWGRASGPFYKSPDEARFVNRLRDMLVLESGDDLWLASGTP
                     RRWLASKEGIRVDEINSYFGPLAFTMHAGDEPNTVVATVRPPTRNAPKEVWLYVRVPD
                     AKRIRKVEINGKEWTDVDPKHERIRLPKTGEVVNVIVQY"
BASE COUNT         6674 a        11371 c        11037 g         6969 t
ORIGIN      
        1 ttcgccaagg gagttttcgc gcttgacaag acgggggcta atgggtgaac gctgatagct
       61 gacagcttct ttactgcagc ttctccaaca gccacaggat attcttcgcc agctcgtcca
      121 gcgtggccat cgcctcctga tcaccggcga tttcgccttc acgcagacca tggccgacgt
      181 tccagtagct gctgccgggc acgatcatgc cgttgatgag aaaccacatg ttgagttgtg
      241 cgaaggtgaa caactcgccg gctcgccggg cgggcacgac cgccgcaccg atcttccggg
      301 ccaggaactg gtcgcccaca cggctgacaa agccgacgcg atccatgtag gctttgatct
      361 gcccggtcac cgatccgaag tacaccggcg agccgagcag gatcacgtcg gcggcccgcg
      421 accgatcgat caagccgttg catgcgtcat cgtgcccatg gcagcgacca tccttaactt
      481 ccgcgcacac catgcatgcc ctgcaaggag cgatgcccat ttcctcaagg cagacgtcgt
      541 cgatttcatg tcctgctttc gacagatgac cgcaaacccg ctgcaacatc agatgagtat
      601 tgcctttggg tctcggactt ccgttgacgc tgagcactcg catggttacc tccttgccga
      661 gagtaactcc cggaacacca ccaagcggac tctcgacctg ggatattaga cctgagagat
      721 gccggtccga caacaggacc gggatcaaat acccggaggc taccgtcggc cccgtgccgc
      781 ttggacgcgg atttgcggat tcggatcgag tcggaccaca aagtcggcgt tggcctccgt
      841 ctcttcgaca gcagcaatat tgatcagatc gttggtctcg aacttctcac gggcttgctc
      901 gggtgaggct ccgccgcgaa tgtgccgctt gatgatccgc tcgcgagcaa cggccaaatc
      961 cgcctcaagg aaaagccttg gccgcagcag agatgagacc ttgtcccagg ggggcgtggt
     1021 gctcaggacg aagttgccct caacgagcac gatctgcgtc cgggcgtcga tcacaatcgc
     1081 accaggcacc ggatcatgca gcctccgatc gtaggccggc aggttgatgg agcggcgcgc
     1141 aggcctcagc gactccaacg cgtcggccat ggccgggacg ttgaaggatt gtggtccgcc
     1201 cttgcgactc cgtagcggca ccggatgtcc tctttcatcg agcacggtgc gcgcgtcgag
     1261 cacgacattg ggatagtgcc agccgtccat ccccaccacg gccagccgtc cggacggcca
     1321 gatgcgaccg gccacaaggg ctgcagcggc ggcgaacgtc gatttgccgc tgcccggtat
     1381 cccgcccaag ccggccagga ctcgctcttc gtcgtcgcca ctgtgttgaa ccagcatcgc
     1441 cagcaacggc agatagacaa gatcgagctg ttcgaccgta atatgaacct ccacctcata
     1501 gccggcaacg ctcagcgccc ggctcccggc aagatcccgt gtgagcccca cccattcagg
     1561 gggatacaca aatgggttct gaggctcatc gatgaccatc gggtctgcct gcttagacgt
     1621 tcaaggttac ctgcttgtta cgcgcggacg aatgccgtct cagaatcggc ccgatcaccg
     1681 tcttaacgaa atgatcgacc tgctgtgggg cgagcccgac gaaccgtctt gcgtcaaggg
     1741 ccttgcggaa gtcaacgttc gcgaacgccg ggtccttctg tagccgctgg agcaggtcgt
     1801 tcggacgccc gtgcatcttg acctccagtg cggcggcctg cgaatgccgt cggatcagct
     1861 cgtgcagtgc ctgccggtca ccggggcggg cccgcttgcc tcttgcgccc tgcccaccgg
     1921 cggtagccgc cattaggatc tcttccgtcg ccatgaaagg cagctccgct tgcacgtgtg
     1981 ccccgatcac tttggggtag acaaccaggc cgcgcgagac gtccgtcaca agcctcagga
     2041 ttccgtcaac ggccaggaaa ctctccggga tcgagagccg cttgttggat gaatcgtcga
     2101 gcgtcctctc caaccactgc tcggcggccg tctgcaaagg cgacgacatc actgagatga
     2161 cgtatcgggc cagtccggtc atccgctcgc accgcatcgg gttgcgcttg taggccatgg
     2221 ccgacgagcc cacctgcgat ttctcaaacg gctcttcgat ctctttcatg ccggccagca
     2281 gacggatgtc gttggcgaac ttgtgggcgc tggcggcaat ccccgccagc gtggcaacta
     2341 cctgggcgtc gatcttgcgg gagtaggtct ggccggtaac cggctcgacg tcctcgaaac
     2401 cgagcttgcg ggtgacgatc ttctccaggc ggaccacctt ggccgcgttg ccgtcaaaca
     2461 gcgaaaggaa gctggcctgc gtgccggtcg ccccgcgaat cccacgaaat cgcagccgcc
     2521 ccaggcgatc ttccagttcc tccaagtcgc gaacaaaatc gtaggcccac aggcaggccc
     2581 gccggccgac cgtggtgagc tgcgccggct gatagtgagt gaaacccaga cagggcaggt
     2641 tgcgccactt gcgggcgaac gcgcccaggg cgtcgatcgc gttgaccagc caatcgcgga
     2701 tcagcaccat cgcctcacgc ataatcagca ggtcggcgtt gtccaccacg tccatgctcg
     2761 tcatgcccag gtgcagaatg ccccgggctt tgggtgccac gtcgccccag gcgtacaggt
     2821 gggccatgac gtcatggcga agcttcttct cgtgctcggc cgctcggtcc cagtcgatgt
     2881 cctccatcgt ccgccgcaac tcggcgatct gccgtcgcga aaccggcagt ccgagctggg
     2941 cctgggcctc agccaccgcc aaccagatcc gccgccaggt cagtatccgg tgccgcgggc
     3001 tgaacagccg gcacatctcc ggcgaggcat tccgggaaac caacgggttc tcgtacgtgt
     3061 ctttgttgct gttcatggtc cgtgattcca gacgattgcc gctctccgtg ccgagtgcca
     3121 accacaccct tgcagatcgc ccggccaggg gcgaaggtgt ttagccaggg gcgcgagccc
     3181 ctgggaatcg tgccgcatcg gcgggtcagc cccagcgggg cgacagatgc tcgtggccgg
     3241 ctcggccgtg ccaccgcccc ttcggggctg gtcggtcatg atggtatttg taccaggggc
     3301 tcgcgcccct ggctacatac ccgtgcccct ttgggaaacg aaggcggttg ggtaccgcgc
     3361 cgacgtgagt ttagttgaga agacaacagg ggtcaaaaga ccgggtcttc aggtacaatc
     3421 gacatcatga gaaagggccg ccttgctcca tcgcgtctgt ttccagaatt ggctctcttt
     3481 gagactgatc aggaacgacg cgacgttatc cggcgatatt ggcgcgaaaa agtgggccgc
     3541 cgtcaacgcc gcttctatct tgcagctctc ttgtttggct cggcggtagc catgggtctg
     3601 accattctag cactcaagcg atcgctggct ttctggggac tctcgggtga aggctgggtt
     3661 ggaggggtga ttgcaggggt ctatccattt gccgttgcta ttggcagtca gtatcttgtc
     3721 cgtaaacctc tgatcaggta cctgcgtgaa cagttggccg ccaagggcgt ccccatctgc
     3781 attgagtgcg gctacgatct ccgaggccag gtcgacccgc gctgccccga gtgcgggacg
     3841 gcatttgacc cgaaactgat caagcgcccg agtgatccgg cagccggccc gtcagactga
     3901 gcgcgctcga cacccaagtg ccgacggagc catcggcacc aagtagggcc gatgcttgcg
     3961 gcgtgccgcc aagtagggcc gacgccctcg tcggcccacc actgtagaac aacggccctg
     4021 ttggcccgtg ggggacccaa tcatgccgcg tgcttttgac tggcaccgag catggatgtc
     4081 acacaagccg tacacgtagg gccgacgccc tcgtcggccc atttcggcat gagtaacacg
     4141 gccgtcgacc tcttcgatcg ccgccggcca cccgacgccc tcgtcgcccc atttcggcat
     4201 taccccccca ttacacaaca atccccgcag ccctgcagaa aagcacgttt ggatcggccg
     4261 tttggcaccg aatcaccatc gtgcccagaa ttcaccagca gtgagcttca tgagcgacgg
     4321 caaagcacgc cccatcctca attctgcctt ccagactctt cattccccgg cgttgccata
     4381 tgcaacgccg ggctatactc tctgcacaac cgaccaaacc ggcagtccaa gcgtgctcga
     4441 tacccaagcg ccgacggagc catcggcacc aagtagggcc gacgccctcg tcggcccacc
     4501 actgtagaac aacggccctg ttggcccgtg ggggacccaa tcatgccgcg tgcttttgac
     4561 tggcgccgag catggatgtc acacaagggc tctctcccgc acttgcggca ggagggagtg
     4621 gtgtacttcg tcacctttcg gttggccgat tcactccctg ccgaagtgat gaaacgactg
     4681 cgcgccaagc gcgatgcatg gctggcatcc catccgccgc ctctgaccga ggagcaaatc
     4741 caggagtacc gaaggatctg gacggctcgg atcgagaaac tgctggacag cgggcatggc
     4801 gagtgtgttc ttcgggatcc cgcgtgccgc aaaccagtcg aggcaaccct gcgacacgat
     4861 gacggcaagg cttaccttct gggtcgcttc gcgatcatgc ccaaccatgt tcacgccctc
     4921 ctgcagttgc tcgccgacag cgatctgtcc aacacgctgc aagcctggaa gtcgatcagc
     4981 gcccggcaga tcaacacgta tcttcgtcga tccggatcgc tctggcagga tgagtccttc
     5041 gaccacatcg tgcgcgacga cgccgccatg aaccggttga cgaagtacat cgaggacaac
     5101 ccccgctgcc tcccggttgg cagctacacc gttggaaccg gcctgcttca ggcacagtaa
     5161 acccgacgcc tggggcgacc catcaagcag gcccaacgtc cgcggcgtgc cgccaagtag
     5221 ggccgacgct tgcggcgggc catcaagtag ggccgacgcc ctcgtcggcc caccactgta
     5281 gaacaacggc cctgttggcc cgtgggggac ccaatcatgc cgcgtgcttt tgactggcac
     5341 cgagcatgga tgtcacacaa gccgtacacg tagggccgac gccctcgtcg gcccatctcg
     5401 gcacgagtaa cacggccgtc gacctcttcg atcgccgccg gccaccatac gccctcgtcg
     5461 gcccattcat tctcccgtgt tccgctccga tcagctctct ccggaacacc cgccgtgtta
     5521 cccctgtatc cagaaaccgg gagacactgc gctctttgac aagtgaacta ccgcgtagga
     5581 agcagccacc agccgcagag gcgagcgtct ggccaagcaa atctggccgc tcactcctga
     5641 tagcttctta ccaaagaaac ccaaaaccag gcaggctggg caaaatggag aatctccgct
     5701 catgcgctcg accatcggca ttccacgggc caaaactctg cccagccaaa tctgacccac
     5761 aaccgcaagt cgttaaccgc caactcgttt tgcggaacga agccaaactt ggatagtctg
     5821 ggaaagatag agaatcccca ctctcaaact cgccgttccg atcttcacaa ccacccaacg
     5881 gggatcgcat tgacattttc gccgagcgga aggagatcct tcgacaggca gacgacaccg
     5941 gcctcaccga ccttcatctt gagcttgccg agcgggttga agaatgccgc ccaatccggc
     6001 cccggtgacg cggatttctt gacctcgagt ggatacaggg tgcggttctg caggaagatg
     6061 agatcgatct ccttgcgatc tttgtcccgg tagtagtaga cctcaggact ttgacctcga
     6121 tgccaccagc tcttgagaac ctcgctcacg acgtaagtct ccaggacggc gccggacatc
     6181 gccccgcttt cgagtgtttc cggcgacgac cagtccgtga ggtaagagca cagcccggta
     6241 tccaggaagt agagcttggg cgccttgacc agccgcttgg tcacgtttgt gtggtacggc
     6301 gaaagcagga acacctgcat gctgccccgg agaatggaaa gccagctctt ggccgtgttg
     6361 acgctgatat cggcgtcgcg agccagatcg gaaagattca gcaggttgcc cgtccgggcg
     6421 gcacatgcct tgaggaacct caggaaggcc gcttcgtctc caacctgcgt caggtccttc
     6481 acgtcccgct gtagataggt ctggagatac gaactgtaga atagatcgcg gtccttcacc
     6541 ggcccggcgg tcaaggcggg gaatccgccc aaccagatat cacgatagac gtgcttgagc
     6601 gatgaagacg catgtgagcc ctcgcgttcc ttgagccgtt ggcgtgtggg taggaacggg
     6661 gcgacatcca accggagccg gtgccgttcc cgagaggaga agccaaggag attgacaatc
     6721 gccacccggc cggccagcgt ttccgaaacg cccttcatga cgtgaaactg ctgcgagccc
     6781 gtcagccaga acaggccggt cttacgccgg gaatcggctg ccatcttgat gtagggcagc
     6841 aactgcggcg cgtattggat ctcgtcgatc aggacgggcg gctcaaacct ctccagaaac
     6901 agcgccggat cctggactgc cagagcccgg atacccgggt catcgagcgt cacgtacctg
     6961 cggccgcgtt ccgccgtatg ctgtaaaaag gtggtcttgc cgacctgtcg cggaccggtc
     7021 agcagcagca cgggaaactg ttccgcagcc ttccgccaag cgtcgtcgat ggctcggggg
     7081 tagtacatgg ccaattcctt tatcggctaa tttgcaatct aaatgcaaaa tagccgatac
     7141 gggccacccg gtcaaccccg accccgcatc cccgccgctt cccccttggc agccacgccg
     7201 ttgaacgcag tctgcttcaa gcacagtagg gccgacgccc gggacgggcc gcacgtaggg
     7261 tcgatgcttg cggcgggcca tcaagtaggg ccgacgcccc cgtcgcccca tcaagtaggg
     7321 ccgacgccct cgtcggccca tttcggcacg agtaacacgg ccgtcgacct cttcgatcac
     7381 cgccggccac ccgacgccct cgtcggccca tttccgctgc aaccagtgtg ctcctcgatc
     7441 actcgacccg tccggcaagg aggattcacc caacacccaa gcgccgacgg agccatcggc
     7501 accaagtagg gccgacgccc tcgtcggccc accactgtag aacaacggcc ctgttggccc
     7561 gtgggggacc caatcatgcc gcgtgctttt gactggcacc gagcatggat gtcacacaag
     7621 ccgtacacgt agggccgacg ccctcgtcgg cccatttcgg catgagtaac acggccgtcg
     7681 acctcttcga tcaccgccgg ccacccgacg cccccgtcgc cccatttccg ctgcaaccag
     7741 cgtgctcctc gatcacccgg cccgtccagg aaggaggctc tgcacaacac ccacgtgccc
     7801 acccggccat cggcaccact tcattgcgct tctggcagcg cggaccggta ctcatccttc
     7861 tgcagcccga tcttctccac cgggatgcgc tggaaaccct tggcccacat gggtgaatcg
     7921 ggcttgagct ggaaccggtt ggcctcgccg tccttgaggt agacttcctc gtcgatggag
     7981 ttgtcctgca tggcaaccac cttgtcggtc aggccgtcgt gcagatcgat ggccttgccg
     8041 cagccgacca ttacgttgcg aaccagctcg ttgtacttgg ccagggctgg ctcatcctgg
     8101 taaagcgtaa gcaattgcgg gtaccgcgtg ctccacggtg gctccttgta gggaaccgca
     8161 ttgagccgtt ccaccagcgt gttatccgtc cggtcgaagt agtacttggc ccagcccaaa
     8221 ccgcgcgagt cgacgtggat gccaatgttg cagtccacaa gaatgttgtt ctccaccgtg
     8281 ttgtcacgtc caccgccgac gagcaccgcc cgaaagacct tgtagcagat gttgccatgg
     8341 accttcacat cgctggccca gtcatcgagg tagatggcct gtgtgccgac ccagctcttg
     8401 aactcgccga tgtggtggaa gtagttgtgg cgaatcacgt tccctcgcca ggtccagtcg
     8461 cggcccatgt agaacgcccc ggcgtcgtcg gtttccatga ccacatggtg cacctcgttc
     8521 agttcaatca cgtgttcgtt gccgctcagg cccagggcca tgtgcggggc gtcgtggatc
     8581 agattgttgg ccgcccggca accgacgccg gccaggctga cggccgtgcg gtaggtgttg
     8641 ctgttgcggc tgtagcggta gatgtggttg ttggtcgcaa aatggccgcc ggggatgagc
     8701 ttcgcgcggt cgccgccgct gaggctgatc ccgccgtcgc cggtgtcgta gatgtcgcaa
     8761 ccggccacgc cgtgcttctc accgccgttg accacgaccg cctgcccgcc gatattgcgt
     8821 aacgtgcatc cggcgacgag gtcccgcttg ccgcccttga tggtaatcgc cgttccacga
     8881 acgtactcga ataccagccc gcggaaggtc acatcggtga cgttctccat cctgaccgct
     8941 gtccccagca ccgacacgta cgtctcgttt gtcgccaagc ccgccggagg ccagaagtag
     9001 agggtgccgc tcctgcgatc cacgtaccat tcacccgggc tgtcgatctc cgagagaaga
     9061 ttgatcgcgt agtagcgctg ctccttacga tacccgtagt ggtgatacgg cgtggccagg
     9121 ttgatgatgc gcttttcagt atcgatcgac tcaaccttct ggtacgcgtc ggcccagtcc
     9181 cagaaccagt aaccgtgcag ccagatgtcg ctctcgccga tccaacgttt cggccgatcg
     9241 ccctcgtacg tgaacttgcc gacggaatcg ccttccttcc cgtggctctt gatcggcttg
     9301 ccgcccacga cgtccgcgat cttggtgaag ccctcgttcg gccatcgcga caactgcatc
     9361 ggcttgccgc cgaagaacaa ttcgatgcgc ttggccgcgg ccacgggatc gccaaagtcg
     9421 gtcacaccgg cggccttgag atcggcctgc atgaccttgc cgcgtgccgc cggttcaagc
     9481 cggtcgagaa tcgcccgatc ggtaacaggt ctgaaatcgg agaccctctt gccgcccagc
     9541 agacgcacct tctcatcctt gtacgcccgg tatgcaatcg gcatcttctc gctgccgctg
     9601 tcctcggcca caagctcaaa ggtcttgtcg agatggtaat ccccgccgcg gatccagaca
     9661 gtgacgccgg ccggcgactc acccggcttc ttcttcatct tgcggatctc gtccctggcc
     9721 cgctccagcg tcgcaaaggg cccatcggtc ttctgctcat tcggcgcggc aagcccgccg
     9781 gaccacgcgt cgttgccgtt ggcggcgacg tatagttccg cagccgattg cgtctgggtc
     9841 gcaagaatca tgacaagcga gaaaagcagc gtgttgcaca tgaccaacat cctccatctc
     9901 agcgagttat ctgaggtgtg tcactgctct gcgagcagta acgtcttctc gcactgccga
     9961 cacggcagtt gcacacgcaa accgtacact tgcggaaaga atacgggcct cccgccgctt
    10021 gtgccagagg gaggcccgtt cacgtttctc aagtagggca gcgtcgagcc aagcgagacc
    10081 cgccgtttca acaagaaggc gggtctcggt cgccgtggcg acctcaacta tgccctaccg
    10141 gagttctttc acccgggcaa cgatattctg tacgccgagg cccagggcgg ctaggacgat
    10201 gtcgccatcc ttgccggact tgggaatccg cgggcaggtc atggcaaaaa ccttcacgtc
    10261 gccgccgcga gcggcggcct cggccagtgc ggcattgaaa ccgccggcga aattgtcctc
    10321 gacggtcaga atcctgccgt tggccttccg cgcggcggtg agaaccggct cggcatctat
    10381 tggcaggcag taagcatcga agaggttgca cttgatcccg gccttctcca attcgcgaac
    10441 cgccttcaaa gccagcgtcg tcatgtagcc gcttgccacg atggtcacgg agtcaccctc
    10501 ggccagtttg ccgcatccgc cgagcttgaa aaccgcatcc tgcgggtaga tcagcgggac
    10561 atccggacgg tgcgtgcgga cgtagcacag accgttgatg ttggcggcca tctcggtcag
    10621 gcggtaggcg gccaccgcat cagccggatg gaagagggtc agcgccgggt tgccgcgccc
    10681 gtcttccgcc gtggtgtacg ctcggaaata cgccatgtcg ggcaggctca tctggctggg
    10741 accgtcagcg ccgagcgagc agccggcgtg cgaaccgacg agtttgacgt tcgcccgagt
    10801 gatcgcggcc atttcaaact ggtcataccc gcgggccagg aacttggcga aactgctggc
    10861 aaaggggatg taaccggcgg ccgagagacc cgcggccgtc gagatcatgt tctgctcggc
    10921 gattttgcac tcgaagaaac ggttcgggaa cgccttggca aagacgttgg cgaaagtcga
    10981 gttgctgacg tcgccgtcca gagcaacgat tcgttcatcg gcgtgaccaa gggcgaacag
    11041 cgctgctcca taggctcgcc gcgtcgagag cttgttcttg gccaccgcct cgatcattcc
    11101 ggtcttggcc acggcctcgg caaacggcat gaccgcaatc ttgtcccttt tcgccggacc
    11161 gttggccgag ggcggcatcg gcggcttggg cgccgggccg cagaccttgg ggagcttcag
    11221 ttcggcagcc accttgtcca ggctggcctc agcctcggcg acctgcgcgg ccgtcagcgg
    11281 cttgccgtgg ttggacttat ccttgagcga atcgacgccc cagcccttct gcgtgcgggc
    11341 cacgatggca accggaaccg agccctttcc aaccttggcg accgccgaag cgatctggtc
    11401 catcttgtgg ccgtcgatgt cctgcacgtc gtagccgaag gccttgagtt tacccaccaa
    11461 cacgtcggcc gactgctgct tggacacgta atccgcctgc ccttggccgt tgcagttgaa
    11521 gatcgggcag acgttgacca gctcgtggtc gatgatgaag tcgagagcct cccagatctg
    11581 cccctcgcgc gactcgccgt cgccgatgat cacgaaaatc cgcttgtcga tcttgctgag
    11641 ccgagcgcca agtgccaggc cggcaccaac cgacaacccc tgcccgagcg agccggtggc
    11701 cgcgtcgaag aacggaaatc cttcggccgg gttcgggtgg ccgtcgagaa cactgtcgag
    11761 ttcgcgaagg ctcttgaggt catcgagcgt cagtgcccgt ttcgcggccg gagacttgcc
    11821 gaccaccccg cccagatccg catatgcggc atagacgatg ggtaccgaat gtccttcgct
    11881 caaaaccagc cggtcgctgg ccgggttcca cgggtcggcc gggtcgtaac gcatctgctc
    11941 gtacatcagc gcggcgacga tatgggccag cgacagagca ctggacgggt gccccgaacc
    12001 ggcggcggtg gtcattctca caacgtgctt gcccaactca atgacgcggg attccaacgg
    12061 attgctcatc ctaaattcct gccagctata acattacaat cgcttggccc cgatgaccga
    12121 cagcggatgc gacagcttag cgggtcagga gcaccctttc aagtggacgc cgggccggct
    12181 gtcctcgcaa accgacaaca ccttccgttg cggctctcca ggagcccgag tatcgtcggt
    12241 cttcggcaac cgacatgggc actatccttc cctggcgtga agcgaggttg acgacatgaa
    12301 ctgcaagtgg ctcgtcggcg caatcggctt gttggggacc gtgctggcac tggctgccca
    12361 tgccgccgag aaggtcccgt tgccggagtc gtcctatgcg ctgccgaaca acttctacgg
    12421 ggcgcacttg ttggtcggcg acggcgctga cggcacgaag ggcgataagc accttcgctg
    12481 ggcgcggttt ctggtcgggc gatggggcta tgccaagacc ttgctggcgg acattgacaa
    12541 gaacaccaaa cagccgccac agggctggat cgattacgtc aacaaatgct atcagctcga
    12601 gttgatcccc gtactgcgac ttgggggcca ctacggtggc gggggctggc gaaaacccga
    12661 ggccgactcc ccgggcgatt acacctctat cgcacaggcg gtcaaacgcg tggtggccgg
    12721 cctgccgcgg acggatatgt gcccgcttta catcgaggtg tggaacgagc cgaacctggc
    12781 ggtcgaatgg agcggcaaag ccaaccccca ggagtacgcg gacttcttcg tggcggtggc
    12841 caaggcaatt cgctccatcg gcgacccgcg aatcaagatc ctcaacggcg gtttggcgac
    12901 cagccccgag tgggccgagg ccctctgcaa agccaacacc gaattcatca gttcgttcga
    12961 cctgtggtcg tgccatccct acccgatgaa ccgcccaccg cacttgaatc atcacaacaa
    13021 gaatatcaaa ccggactccg aactaagcat cgacagctac ctgctcgagt tggccaaact
    13081 gcgggagtgc ggccgcgaca acgtcaagat catgatcacg gaaaccgggt gggacctggg
    13141 caacgccgtg tacaccaagt cagaagggca cccgatcatc gacgagtaca accgcgcgga
    13201 ttacgcgatg cgggccttcc gcgactacta cccgaactgg cccgaactcg tggcggtctt
    13261 tcccttcgag ttctccaacg agggttggca gcagttcgat tacgtgtatc ccgaaagcgg
    13321 catcaatccg gacggctcgc ccaccaagcc gcattaccag tacacggtgg tcgcggctct
    13381 ggccaagccg accgacacca cgggggcaat cagcggaaca gtgacggtcg ccggcctgga
    13441 cgttcggctc gacggtgccg aagtgagcat cggcggaaag cccttcacat ccgacccgat
    13501 gggcaactac ttcttcggca ggcttgaacc gggctcttac agcctgagca tcagcaagcc
    13561 cggcttcaag gctctcaaac agaaggttca ggtcgccgcg gcgaagaaca gcgtcgtgga
    13621 cgttgccctg gaagcgaccc tcaagaccac gctcagcggg gtggtacgca gcggtgatga
    13681 tgacaaaccc gtggctgggg cggtgatcac gctccagccg ggcaaacacg aggccaggac
    13741 cgactctgac ggccggtaca ttctcgagaa tgtcatcccc gcccgctaca agctgtctgc
    13801 cacggcccag ggtcgctata agtacgaggc ggccgatgtc cgcatctcca gcgagcagga
    13861 gaaccggcat gacctgcggc ttggtcagct cgcgaagctg ccgcccggtg agaacatgct
    13921 gaacaacgcg agcatggaag ccggtggcgg cggcggaggc aaggaatgga tcgccctggg
    13981 ctttgagacg ctgaaggtcg acggagacgt gatgagatca aaccccggcg gggtcgccga
    14041 caagacagcc cacaccgggc ggctttcgca aagcatgcga gtgtcaccga aggaaaccgt
    14101 catccgtcag atcacgcact acaacactgc cagggtgggc acgaagtatt tcgcgggcat
    14161 ctggatcaag accgactgcc cggatgacca gggggccgcc tggatcacat tcgatgccac
    14221 cgacaacagc ggcggtgttg tcgggcgtct cggtccgagc cgcaaggtca ccggacgcag
    14281 cgagtggaca tgggtatccc tggaaggccc ggcccccaaa ggcagcgagc ggctttcgct
    14341 caatttgcac acccaaggca agtcgggtgt cgcctatttt gatgacgcgt tcgtgggagt
    14401 ggtgacgccg gcgaggtgag accgtgagac tttttgtgac cggtgcggcg gggcgactgg
    14461 gcttgcgcgt gctggagcgg ttttcgcctt cgcacgacat cgtcgcgttt gaccgtgccg
    14521 acccgccgag aagcctgccc gcgagcgttc gctacgttca ggccgatctt cgagacgcaa
    14581 gagccgttcg cgaagcctgc gccgggtgcc aggctgtggt tcacctcgcc gcgatcccca
    14641 gtcggatgcc gacgatcccg caatccgaga tcttcgccat caacacgcag gggacgtacc
    14701 acgttctcga ggcggccgac cgcgccggag cgggcatggt tgttctggcc ggcagcctgt
    14761 gcgcgatcgg gctgccgacg tcgctggaga atcatggtct gtcgtacctg ccgatcgacg
    14821 aggatcaccc ttgccgaccg aatcatgcct acgacctgag caagcggctc aacgagctga
    14881 ccgccgagac cttctcgcgt ttgagcggtg tgcccacgat ctgtctgcgg ttcccggctc
    14941 tgatcgacgt tcgacacgat aagtggtttg ccagggagat tcacagctcg tcgccgagac
    15001 tggtgctggg tgattacctc gatttctccg acgcggtcgg cgcaatcgag acggccctgc
    15061 gccgccgcga cctcaagcat gaacttctgt tcgtcaacgc cgagacgatc gggaccgcta
    15121 cacccacagc ccagtacgtg cagagattca acccacgagt cgaatggcgc gggccggctc
    15181 cgaatgccac cacccctctg atcaactgcg cccggctcaa gcgcgtgctc aacttcaccc
    15241 cggccgtcac ctggcagcag gacatggccg tgtcagggta agtgcggagg aaaccatgtt
    15301 cacgtgcgtc ggtcgtgttg agtaccccac cactgcttcg agacccgcgg gcggcaacgc
    15361 tccactgctt gctttgatga tcttgccgac aacgctgctt gcgggtctga cgccctgtct
    15421 tgccgacacc gccagtcaac cggccacccc cagccaggat gtcgccaggt tcgtcgtccc
    15481 tgttcgctgg gaacgatttg cgtcaccgaa cttacgcaac aaggatgtgc agaccgctgc
    15541 gcggatgctg ctcaacagtg tccgctataa cctggcctgg gccggcgtgg cactgaagga
    15601 ggccccggag ggcgactgtt acctgatcac cgatatgaac gaacccggta ttcgcccgcc
    15661 ggccagcgcg gcgtgcggga tcgccacggc catcgcggcc ggtatctacg acgagaaaac
    15721 ctgcggcatg ctgaagggcc aagccctgtc acggacgctc aagatcatca gagatgtcgc
    15781 tgccgttcac agagtcaacg gtgacccggc taaaggctgg ggcgaccatt ggcaatcggc
    15841 gctctgggcg atgctggccg gccgtgccgg ctggatgctc tgggagcacc tcgatccgcc
    15901 aacccggcac atgaccgcgg cgatgatcga atacgaagcc gaccggttca ttcgcccggg
    15961 ctacaccgtg ccttactgga acggcaaagg cggcgacagc aaagccgagg aaaacgcctg
    16021 gaacgcgacg gtgctgcagt tggcgatcgc gatgatgccg cggcacccgc gcgtaccgca
    16081 gtggaaggag atcgccagcg agttgatgat ctcggcgttc gctcgggaag aggacatgaa
    16141 atcgaacacc acgatgctcg acggtcggcc ggtgaaagag tggctcaaag gctacaacat
    16201 ccgcgacgac ggtgccctgg tcaaccacaa catcatccac tgcgactaca tgaccacggt
    16261 cacgctgaac ctgcacgcgt tgttgctctg ttcgctggcg ggccaaccgg cgccgcagag
    16321 tgccgacttc aacgccgcac tggtttaccg gtcgctgatc gagcagaact ggccttcgcc
    16381 gccgtacagg ccacctggcg ggactatgta cgtccccggc aaggcggagg tctactaccc
    16441 cgaaggcaca gactggagcg actatcgctt cgacatcttc tactggatcg actcttacgc
    16501 gcacctgttc ggctgggaca agggtttgag ccaccaggcg agcgattgga tgcggctgcg
    16561 ggccgggcgg attctggaga tgcaggggcg tcaccccgac ggccgcatgt tcgctaaggg
    16621 cgagttcgag acctatggcg gggccgagca gatgacctgc tgggtaatgg ccgacgcatt
    16681 cctcttgcac tggctccatg acgcccaaac gctgagccga caggctgact ggaatcagcc
    16741 ttgagagcgc gccgcaaatc cacggcccgc ctatatccta tcaggcatct tgccggtggc
    16801 tcccacggct tgcgggatta ccctggtatg agctaccatt gcggcatgat ggtggggggt
    16861 tcttggtccc gatgtgacgc cggcgcgggc aacgggttcc caggacgcgt gtgaaaaggg
    16921 tagaacacgg tcgcagccct gcgaagtctc atggacgaag caggggcgga gtcctgccga
    16981 ccgagcttgg caatacggca ggtctcggtc gccgcggcga cctcgacgct gccccaccga
    17041 atgctcgcgg ctgttttcac acatgccccg agggtcgcgc accagcagca ggcggcattt
    17101 catgtagccg tgcaccgccg cggttcggga gtcggtgatg gagttcttgt gcatcctgct
    17161 ctcggtgttg ttgttcgtca ctctgatcgg acacggcctg tgggtcgcca tcgcatggct
    17221 tctgcgaggc ggccatccca agagcgaccg gcgcagcttc gagccgacgc tggatgacga
    17281 ccgggcggcg acggcacgct acctcaccca tctgcaggcc cgaggtctga tcgacgccga
    17341 gactcacgca cggctggcga agctcatggc ggaagacgtg cattccgtcc gagcgtattt
    17401 ggacaaaaaa cacacccccg ccaagacaac ccagcccacg gcagaaccgt ggcagaggcc
    17461 ggcaggcata caacagcagc ctgccgcgcc ggtcgcttca tcgcccgctg agagcttgcc
    17521 gtgggcgggg gcgacggaga gcgcgcctgt tcctgcactg gcaggcaagc ccctcgacac
    17581 aggctcggga cagaagccgc cagtggctcc cggcggaacg attggtgcag cagcacctcc
    17641 gccggtgact tcattaccga ccgagccgcc gtcaccggct gaacctcgcc ggccgttcgg
    17701 cgaggttgtg gccgcattca tggctgagaa gaacatccgc tggggcgaac tggtcggcgg
    17761 gcttctgatt ctctgctgct cgaccgccct ggtgatcagt ctgtgggccc agatcgaggc
    17821 catccccatc ctcaagttcg tgatcttcac gaccgtgacc gcggccctgt tcggagtggg
    17881 gctgttcgtg cagcatcggt ggaagctgcc gacgaccggc cacgttgtgc tgattatttc
    17941 gtcgctactg gtaccgttga atcttctggc ttttgccgtg ttttcgccgc gggggccgac
    18001 gatcaacgcc tggaccttga tcgtggagct gccggccgtg gccttgttcg cctggctgac
    18061 cttgctggca ggccgcgtca ttatgtcgga tggggcaaga ctgttcgcgt ccggaatcgt
    18121 ggtgctatcg gtatgctcac tggtcatccg ctttctgtcg ccgttgtccg ccagcggact
    18181 tgtggccgtg gggctgctgc cggtcgggtg ttacgtcatc gtgatgggca tttctctgcg
    18241 acggcaatca cgagtcgcat gcatcgagga cacggacgcc aagaggctga tcctgcaact
    18301 gggagttcaa acgtttgcct gcctggtgcc cttggggctg ctgctcggtg aatcggggca
    18361 tctggccggg gcactgcacg tactctcgcc cttgatctgc gccttggccg cacccgcact
    18421 gctggccggc cttctgctgg tcagtcgact ggcgcccgca gtgcctgctc aaacgcgttt
    18481 gaccgctaca agcatcgccc tggtcgcggc cgcggtcatg ctgatcggcg tcgggttggc
    18541 ttggccgacg ccgtcacggt tgctgcccgc gatgcttctg aacgccctgg ccgcggcggc
    18601 cgtttgttcg tcaattcgcc accccgccat gcatgcagcc gtcgcaacgt ggctgacgct
    18661 cgcatggatc ctgggaatcc acatgatttg cggcgcggtt tcgtggtcag aggttgggtc
    18721 gagagcgatg cttgccgcat tgttctccgc ccggacgggc caggcattgg tcgggcctgt
    18781 cgcggcatgt gccgtggccg cggccttgct gcaccgtcgt caacgtcggg aactggcgat
    18841 cggatacgac ctgagtgccc tggttttctc ccttctgagc atcggcgtgg tgacatggtt
    18901 cggcttcggc gtgccgggag atgctcagca cgtcatttgg gtgtacctgg tctacggcgc
    18961 ggccgcgatg gtgggtgccg gtcgactcaa gagcacgcgc gtcgcctggg tcggctgcat
    19021 cctgattcag atggcgatca ttcaggcgat ggtttacgct tggccgctgc aacagttccc
    19081 ctggccgacc gcattgctga tcggggccgg cgcttgcaca gtggcagccg ttgttctgag
    19141 actcgctcgg ctgacaagtg acgccgcgaa cacgtatgtc gactctctgg ccgggttcgc
    19201 gacggtcgga tcgtggctgg ccgtcgtgtg gatgctgaac acgctgccgt cacattcact
    19261 cgccgcgttc tcggcaagga tggcgtggac ctcggtcata tgggctgttg tggcaatcgt
    19321 gaaactatgg cccattctgc tcacgggcgc cgagattgcc ataatcgtgt cgacatgcac
    19381 cgcgctgcaa tgtcaccttc gcagtctgga atggtaccga ctgctctcat caccatttca
    19441 cgacccgtgg gtctggcaag cgcacctgat ggtgatcggc gggttcggct tggtcctgat
    19501 cgtggcccgc caggcgatca cgcaatggtt tgccccactg acaagcgggg acacgccgga
    19561 agcaccggcg ccaagcgacg caggttggcg aagtgtcgca agacagttgt tggagccgtg
    19621 gttccccgca acagaccgtt ggctggtcgc tcttccattt ctcgcacttg tgtttctggc
    19681 cggctggagc gtcgcgcccg cagtggtcgt cgagcatggt gccgcagcct cgaaggtggc
    19741 ctggggtctg cacgctcacg cggccggggc agggtcatgg attctgctgg cgctggtcgc
    19801 agcaacgctc ctgcttcaca tttgggaggg attgcgcaga tgggccttgc ttgggatcat
    19861 tgccgcggcg gcatgtggcc tcctgctggt cgtcgcgagg ttcgagtcgc ggcaccaggt
    19921 cgtcgatgcc tggcgctggc tgtcggcatg cgcgttcctg gccgtgtctg ttgcattctg
    19981 gatgcgtggc tactgggttg acagactgct gcaaatggct cgctgtcgcc ccgcacggca
    20041 atcagggaac accgacatca cccgcgtttg ccttttctgc ctcttcgcgt tgccggccgt
    20101 tctactcacc gcgtcgcaca tggcggccgc cggttccatg aagcttccga tctcgcctga
    20161 cttgaccgag cttcgactgc acgttgcctt gctcggcccg gtggtgcttg tgatcgtttc
    20221 ggtgatcggc tacggcctca gcgagcggca agtcggttat gccgttgcgg ctgccgggtt
    20281 ggcgtgtgcc gccgtgaccg tgaccgagtt gcacattctc agacaaacca agcagccgtt
    20341 ttctgcgggt tttctgatct ggctgacgca attgaacctc attgtctctt ccgtcgttcc
    20401 cattttctgg aaggtgctgc gagatctgtt ccggacgaag gatgatcgct ctgattatcc
    20461 atcggggccg ctgatcgcag gtcgcgtggc cgtcggcatc cttcttgccg tcgcggccac
    20521 cggaatgtgg atcagcccgc agtttctgcc ctcggtcacg gtcgcggccg gcacgttgtg
    20581 gggcattgcg gcggcacttc tcgtcgaata cgcgctgctg acctcgtgcc gcccatcgtc
    20641 ggccaaccag aggggccacc gcgagagcgt gtgggtgctc tacggcgtag cccttattgc
    20701 gtgtgccctg gcgccgctgg atacaggcaa ctggctttgc ttccacgtcg cgatggtcgg
    20761 ttttgtgacc gccgccgcga tcaggctttg ggctggtgac agacaggccc gcgaactggt
    20821 cggagccggc tggcaggaga cgttcgatac cgcctccgcc gacgtgagcg gcgccgttcg
    20881 gcagatcgac catgacctga gttgcacacg atgcggctat aacctgcgag ggctttctcc
    20941 gtccagccga tgccccgagt gcggcctgga gattccgggt tcggtcgaga ccgcggtcag
    21001 caggttgacg ccgcagtcga ccgccgagcg cgcccaggcc cgcgttcaag ccgtgaccgg
    21061 cgttctggcc tgcatcttcc tggcaaccct gttggcgatg cgggcggcta ccgacgaccc
    21121 ccagcgtcca tggtggtcga cgggaaccat gtgtgcattg tcggtgctgt gcataatcac
    21181 tgccgcatgg gcgccccgtc gtgcgctggc ctatctcggc ggcgtcgaga tctgccttgc
    21241 ggcgagcgtc tggtgggtgc ggttcttctg gctcaggctc gctaccttct ctggggcgaa
    21301 tctgatcgac ctggtgaatg tcaacgtcat tgcgctggcg gcggcgggaa tcacttggtt
    21361 gctggtcgag cgccgattcg tgctgcctcg cgtcccttcc ggccacacgg agcgctggcc
    21421 ctcgttccat cacgcggttg cgacggtttc cgtcgtcatc gtgttcttgc tggcgttgcg
    21481 ggcgatctgc ctggcggcca tcggagagcc ggccggcaac tcgattgccg tgagctggct
    21541 ggcgtggacg gcggccgtcg cgatcgtgct ggtctgcggc ctggactcca aatcggtaca
    21601 cttgcctgcg ggcatctacc ttctggggct gacgggcatg agccagatca tggcccagac
    21661 gggcatgacg ccgcgatacc ttgagtgggc gatgtcggtg gggctggccg gttacgcggt
    21721 ggtcacgacc ctgtcctggc ggcgatggac gagaaccggg ccgcttgcag ccgcccggca
    21781 gaccaccgac ggctggttgc cctcagccaa tagcatcctc gcgctgatcg ccatgcttct
    21841 ggccctgcac gtcagttggg gccatcctgc cctggtctgg cgcatgctca tcgtcatatc
    21901 gccgctgctg tgcgccgcat gggctgtgct tacggccgtg gaggcaaggc ggctggttat
    21961 gcagacctgc gctatgcaac tgctggcggc ggctgccatc ctgtttgcct ggtcgtgggt
    22021 ctcgccggag atggcggccg gagtactcca ccgggcggtc ggtctcatcg cggccgtcgc
    22081 cctggccacg ccggttctcg ccgtggtcag aacgcgaatt gcagcgggcg atacatggcc
    22141 gacggccctg actcgttcga tcatcacggc aaacgcgttg gcggtcgccg gactggcgta
    22201 ctgttgcgcc tctgacgcat tgagcatatt gggcgatgac ctcctgccgc tgactcggcc
    22261 ggccgtcgtc gccatggtct tggccctgcc cctgatgatc atctattgca ttctctttgc
    22321 cacgcgcgat cggctcgatc cgctgcagct cgacgcccgc cgcaaagagg cctacgtcta
    22381 tgccgccgag gtgctggccg gcctattaac gctgcacgtc cgcgcttcga tgccctggct
    22441 gttcaccggc gtgatcgagc agtattggcc tctcctggtg atcgctctgg catttgcggc
    22501 ggtggcaggc caggaagcct gcgagcgata cgggcagcac gtgctggccc ggccgttggg
    22561 acgaacgggt gtttttctgc cggcgttgtc gatgctggat ctgttcatcg ccgcgtcgcg
    22621 ggtccacttc tcggtagtgc tgttcacgac gggcgttttg tacatcgtcc ttgccgccac
    22681 gcggcgatcg atggtgatgg ccgtgctcgc ggcctcgtcg ttcaccggca gcctctggta
    22741 tctgctctat cggacgccgg gcatgggcct gaccgagcat ccccagttgt ggttcatccc
    22801 gccggcgata gccgtgctca tcgccacgca cctgaaccgc gaccgtctcg acgagatgca
    22861 gcgaaggacc gtccattacg cgtgcctgct gaccatctac ctttcatcaa ccgcggacat
    22921 ctttctgatc ggcgtggccc aggcgccctg gctgccgctg attctggccc tgctgtcggt
    22981 ggtgggcatc ttcgtcggag tcgcactgcg gatccgctcg ttcctggtgc tcggcaccgg
    23041 gttcctgtgc ctgtcattgc tgaccatgat ctatcacgcg ggcagcaact tgggttgggt
    23101 ctggatatgg tgggcaagcg gcatcgccct gggcatcgcg atcctcttcg tgttcgcctt
    23161 gttcgagaag aaacgcaacg agatggtcgc cctgctcgat caggttcgca agtggcatga
    23221 ctgagaggat cccctggctg gtttctctcc cgtcgcgtgg agaagtcgga agggatcctt
    23281 tcgctaccca tcctcactcc tgccgagcgc ggttccgcta tcacgatggg aatgtgtgct
    23341 ccattgctca ggtcagtcgg ggatttccgg caacttgtgc agccggatcg ggagaaactt
    23401 cgcataccga gcgaaatctt cgtcggtcgt aaagatgggc acatccgcac gagcggcaac
    23461 agcgcagatc agcaggtcga tggcggtacc ggcaaaacct ttgccgcgac aaacattgaa
    23521 gaacgctgcg gcctgatcgt agtcctccgg gaggatttcg atgcagccaa aggcggacag
    23581 ccgcgtgcga accgcgagga agtcggactc tcggcggatc ccggaaagga tctcttgacg
    23641 aataggaccg atcaacaaga tccgacccgc ttcgaccagt gatgcccact cctgaaccag
    23701 cagccgttcg cggcgattga gctccgacgt ccggcgccgc agagccaggg accagatcgt
    23761 cgtgtcaacg agcatgctca ttgccgccgg ccccgccgca gggccttgtg atcgtaatca
    23821 tcccagtatt ccacccgccc ggccagggtg aggatttctc gctgcttgcg gtgctgcacg
    23881 tactccttga gcgccgcggt caccgcgtct ttcttggttt tgtgcttgcc ggccttgcgg
    23941 gcttcctcga tcagcctgtc gtccagtgcc aggttcgtag gcatagctca cctcactttg
    24001 gccccttaat cctacacgat gtcatgtgta ggagcaagcg cgtgtcactt ctgaagcgtg
    24061 ccgagatatc gcaacggaaa aagaatcggg atcccaaagc cttctcgtca gggacgctct
    24121 gcgcaacgcc gcttctactt ctgctgcggc ggtttcttcc cgtcggtttg cccgccgtcc
    24181 aggagactga gcagatcggc ggcctttctg ttggcgacgg tcagggatcg gcggtggcca
    24241 aggtacaaca agcacatcac agcgagcacg acgacggtgc cgatcacagc caccagaacc
    24301 tgccccaagc cgcaggccat gcccatgatc atgctgaaca gcaggatcgt tgtatccttc
    24361 gggtcgcgga cgatggtgcg gtagcggatc agcccgatgg ttcctgccag gccgaaggcc
    24421 cgcacaaggt tgtcgccgac caccagccag atcagcgccc cgcccagaca caagagcagg
    24481 tgcgtgtccg gcagcgtcgg ggcgaaccgc tggccggtat aggttcggcg ataaacatgc
    24541 ccgatcagaa acccgatggc gcacgcggca atcaggcgat aagcaataac gtccgcccca
    24601 agccgcgcgt ccgaggttcc gccaaaggtg tcgctgatga gccgttcgag gtccggatgg
    24661 tcttcgagga agccggcgat ggtgttcacg agatgcacct ctgcgattga agctccagga
    24721 ttcgttgcat gctgcgagca aacttggaga aacgcttgac cctcttgagc cccaggctcc
    24781 tgcgcatctc gcgcagatgc tccggctcat cgccgtggtg cttgctttcg atcaccagac
    24841 cctccaggat gccgctgtag cagccgcagc cgctgacaaa acgcaggttg cggtcaaggg
    24901 tgatccgaaa gctccgggag tccgggccct ggaaggccag gcgctcgtat tcgacccgca
    24961 ccgccggcct gagacgctgt tccaggatca ttgcccgaat cgcactgtag atttgagtaa
    25021 tgtccccctc gttgcagggg gcaacgcgat cgagaacgtc ttcgccgcgc atcaaagcag
    25081 ccaattccgg cttgcggcaa cagaagcggc gtttgaggct gaggcgatgg ttcttgatct
    25141 tgatttccac ccaacacagg ttgctgaact ctccggccac accgtactgg cggatccgga
    25201 tcttctttcg gaggggcaat cggacgaggt attcccgata ggccgcgaaa tcggccgtat
    25261 cgaggtacgt ggtgcggatg ctcgaccagt catgatctcc ggcatagcgg tgtatgggca
    25321 acagttccga caagcgcgcg accgcgagat ccagcatggc atcgggcaat gcatatttgc
    25381 gctcgacacg ctgggaggtg gtggatatcg gtgcttgctc gatctcgaac atcactcacc
    25441 caagccgggg tcaggatccc agggtcccat atccggcggc tgccagatga tggagcctac
    25501 cggattgggc ctgcctgcgt cataatcggc cttgttgtac gacctcaagg ccagcgcctt
    25561 cttcttcatg ttttccacgt gccagtcggc aaacaggagg ttggtggcga tgttatgacg
    25621 gtagttgaca tcgtcattgt gcaatcgcca gcggcccttg aacgacggcg gcggcggtga
    25681 acccggccca agatcgacag ggcctgttcc cacatcgccg tcaaacagga caaccgtgtc
    25741 tgacggccgc ttgagagtct cgacccttcg ataaggcatg aaataatcgt tccgagtgcc
    25801 gtccttttta tagagcgaat agtccgccag cttgctgttc atccggtagc tctcttcacg
    25861 attgggctcg aatcgctgga cggtcgggca gatcttgact tgctcggcag agactccctt
    25921 gcccatgtag cgatccaggc agtcaaacca gcagatccag ccgcggacct gggccggccg
    25981 gccggccgac gcgggcacat cgtcccacac cttgtcgccg ttttcgtcct cgcccatgcc
    26041 tggacgaggg tcgggccgtt cctcgaacgg caggactccg ttggtggaat tgacatacat
    26101 caggatggcc aggccccact gatggagatt gctgcgacag ttgaccgaac gggcgttttc
    26161 gcgactgcgg gacagcgagg gcagcaaaat agacagcaac agcgcgataa cgccaatggt
    26221 gaccatcacc tcgataagtg taaatgccgc tctcttatgg aacctgcagg aggacatcac
    26281 cgtttcgaag ccccgttatc tgacccatcc cggctccctt gactgctccg cgtcattcta
    26341 ttgtaccaca cgccggtccg agttggaaac gcttccatta accgaatagg aaccgaatct
    26401 caacaccacg gactcttccg cactctatgt cgtggacggc cagacgggac tggaatgagg
    26461 gctgcccgaa accaagccct gcatgggggg tagaggacaa gtccgctccg tcaaacggtt
    26521 tccaggtact gcagtgatgc caggaggccg gtctgttcac ccagctcggt accgttgtac
    26581 tcggcgtcct ccagttcaac cgaaacaaca ccgccaaaac ccgattttgc gaggatcgcc
    26641 agaatgtggc tccagcgggc cacgccgtga ccgggaatcg tgtatcgcca cacgaactcg
    26701 ccacagaaga acggtttctg gaagatggac tgctggtata ggccaatctc gtagaggtta
    26761 tcggtaatga tctccgtatc cttggcgtga agatgtatga tccgatcggc gaactcggcg
    26821 gcgaacctgg catggtcgat gcccattcga atgaggtgcg agggatcgta gttgatgccc
    26881 agaccgcggc agggcacctc acggaacatc gcccggcagg tctccggatt gcagcagaga
    26941 ttggcgaacg gcggactgcc gggccagcct tccagtgcga tcgtcgctcc caggctctcg
    27001 gccacagggg ccagatcgcc gtagctggcg acggccaggt cgaagttgtc cttgcccggc
    27061 cgggaggggt cctctgggat ggccacggtg aagaaaacct ggacccccag agtagccatc
    27121 tccttgaaat aggccgcgtt gcgggcgatg gccgaccggc gtttgccggc gtcgggactg
    27181 agcagggccg accagtcgag catgtccacg gataccacct gcaggcccgc atcccgtact
    27241 tgaccgatat cttcgggtct tgcctgcgca agatcgatgg cctcaaaacc cgcatccttg
    27301 gcccaggttg ccaaggcagg caggtctttc tgccaatcgc tccacccgcg acgaaaaccg
    27361 atccgaaaac cgccggtacg tgtgcgcatg gacacctccc aaacagcatg cggctcctgg
    27421 tgtcgcagga taggaggctg gccagaccaa gtcaaaaccg ctcggcccgg aatcccttcc
    27481 gagccgcctc gcctggccga atccgttcgg cattcggtga taatgaggat gggaatcctc
    27541 gacgcagatg gccccagaat ggattctggg accagcaaga ccagaccaag tcaaaagctt
    27601 cacggctgga tttccctgcc gctctccgtt atgaaagtgc cggaacgaga aaaccgaaag
    27661 gagatttgcc tccatgccag acaccattcg cgtcgggatg atcggttgcg acacatcgca
    27721 ctgcggagcg ttcaccaagc tgtttcacga gcaggccccg gccgagggcg tgcccgcggt
    27781 ccgtgtggtc gcgtgctacc cctcattcag cccggacgtc aaaagcagcg ccgatagggt
    27841 tgaggagtac aagaagaacc tggtcgagaa gtggcaagtg aaaatgaccg cctccatcga
    27901 ggagatgctc aaggaggtgg acgtggtgat gatcgagagc gtcgacggcc gtcggcatct
    27961 caaggagctt cggcctgtgg ccgaggccgg caagccggtc tacatcgaca agccctttgc
    28021 cgccagcctg ggcgacgcca aggagatgtt caagatcatc aaggatgcca agctgccggc
    28081 gttcagttgt tcgtcgctgc gattcgacag ccgggtggcc ggttttgccg acctgaccga
    28141 caagcacggc aaggtggttg gctgcgatgc gttcggacct gcgcacctgg agcctacgaa
    28201 tcccgggttc ttctggtacg gcatccacgg tgtcgagatc ctctacacca ttatgggtcg
    28261 cgggtgcagg gcggtgcagt gcatgagcac cgccgacggc gacctggcca tcggtgcgtg
    28321 gaaggacgga cggctgggct cgatgcgcgg catccgcaag ggtaaggccg aatacggggc
    28381 gatggtgctg tgcgaaaaca gcctccagcc gctccaccct gtcggcgact actaccccgg
    28441 tctggtcaaa gagctgatca agttcttcaa taccaagcag ccgccgttcc ccatcgaaga
    28501 cacgcttgaa atctgcgcct tcatcgacgc ggccctgcgg tcggacaaga aggaaggcga
    28561 cgaggtggcg ttggatctgt gagagcacgt gcgaaaacct gctggggcaa gtggtagggc
    28621 agcgtcgagg tcgccccggc gaccgagact tgccgcatag ctgagcttgg gttggcaggt
    28681 ctccgccgcc tccggcggct ccgaccatgc cctaccgttt tcgtacacgc tgtgaggcat
    28741 agaagatggc aaaccagaac acctctcaca gactcctcct cggccacagc cccgacccgg
    28801 atgatgcctt catgttttac gggctggcga ccggacgcgt ggattccgga cgatggcgat
    28861 tcgagcatat tctgcaggat atccagacgc tcaatgaacg tgcgatgcgc ggcgaactgc
    28921 acattaccgc gatcagtatt cacgcctacc cgcacgtggc cggccagtac gccctgacca
    28981 actgcggatc gagtatgggt gacggctacg gtccgatgat cgtcgctcgc cggccgatgg
    29041 acgtggcggc gctcaagggc aagcgcatcg ccgtgcccgg acgcatgacc accgccttcc
    29101 tggctctcaa cctgctgctc ggcaaggatt cctttcagca cgaagtggtc atgttcgatc
    29161 agatcctcgc ccacgtggcc gacggcaagg ccgacgccgg gctgatcatc cacgagggcc
    29221 aactcaccta ccggaacaac aagctgcact gtgtcgtgga tctgggcgtc tggtggaaag
    29281 aactgaccgg cctgccgttg ccgctcgggg gcaatgcgat ccgccgcgat ctcggcgaag
    29341 ggaacatgcg cgaaatcgcc gggatcatca agaacagcat tcaatactcg ctcgatcacc
    29401 gcgatgaggc cgttcagcat gcgatgcagt atgcccgcga catgaaccag tcgctggccg
    29461 atcggtttgt gggcatgtac gtcaatgagt ggacgctgga ctacggcgac acgggccgca
    29521 aggccgttcg cgaactgctc cgccgcggcc acgccgcagg actggtaccc gacgccggac
    29581 ggatcgactt cgtttagccg ccttccattc ccgaaagcgc cggccgacag agtcgcatgg
    29641 gagatgccac gtgcggctga ccccgcggca ttgggtcaat acacgaatgg agacgaatcc
    29701 atgctgtgca aacacgatcg acaggtgcgg ttgcagggca tgaggccggc ctccggcaac
    29761 gcggcgaagg tgagcatcgg gattgccttg ctctcgagcg tgttgttgac ggctgaccag
    29821 tcggcgttcc ccgctgacga tggcttgctg gcgtggtggc ggtttgatga cggcaaaggg
    29881 agaacggccg tcgatgccgt gggccagaca cccgatcaac tgatcggcaa cgtcaagttc
    29941 gccgagggcg tctcgggaac agggctcaaa tgcgatggct tcacgacctg tctgacgcga
    30001 agggcgaaag acgcaccgaa gccaggcgac gcgttcagcg ttgaggcctg gattgccgtg
    30061 caggcttacc cttggggatg gtgcccggtc gtgagtcagc gaaccggtca cacggccggg
    30121 tatttgttcg gcgtggatgc ccgcggccgc gtgggtctgc acatggccgt gggcgggcaa
    30181 tggcgggaat gcacgtcgga ggctatggtc ccgctgatga catgggtcca cgtggccggc
    30241 acgttcgatg caggttcggg gctcaccgtc tacattgacg gggcgaaggc cggcactctt
    30301 gtttcctcag gctcgccaac gtacgccgac gacgtcgacc tgctcatcgg gcgtaaccac
    30361 acgaaagagc ctgccatgtt ctcggccttg tggggggccg ccgatcagcc ggtccacttc
    30421 gccttcgacg gcatcatcga cgaggtgaag atgcacggcc gctgcctgac cgccgacgag
    30481 gtgcgcaagg cgtttcaggc agttcgcccg agcgcgccgc tagacctgaa accgcgccgc
    30541 atgccttcgg ggccgaaaga tatccatcgt ttcggggcgt tctatcatca tctgaagtac
    30601 tgcgaggaat gggacgctat ctggcgaggc aacgggcccg acgtggtcgt ggccttcaag
    30661 ttcgcacccg tgcggctggt cttctggcgg ggtgtctcct acgcaccctg ttgggtgacc
    30721 gagaaggaca actggttcac aaacgagttc atggaacgcg ggcccgatcg caagacacgg
    30781 ggctgctcgg agtcgatgtc ggacaagcgc gcccactact cgcacgtcaa gatccttgaa
    30841 aacagcgacg cgcgggcggt cgtgtactgg cggaacgctc ccgttggcat caactacgaa
    30901 ttcgccttcg tggacgagat cacgggttgg ggcgactggt ccgaggaata tcatacgatc
    30961 taccccgacg gcgtggcggt gcgcaaggtc gtcatgtgga gcagcaactt caaggagtgg
    31021 cacgaatggt gccagtcgat tcagccgttg cacgccggac agcgacccga agacgtgctt
    31081 gacgccaaac gcatcatgtc ggtggcgaac atgaacggcg aaaccaagac gttcggccgg
    31141 gaatcaggcg agaaaggata cggtttcccg accattccgg gggccaacat ccagatcgtc
    31201 tatctggcgt cgaagtacaa tccctttctc gtgcttgatg atcgtgaggg caagaacgat
    31261 cgtggacggc caggtcctga aattgtccgg gcaagcggcg acggctggtc ggagcactcg
    31321 gtattcccct ggggaaacca ctggccggtc acgcagattc cggtgctggc acggtatcca
    31381 caggccgccg accgaccctc gcacacatgg acctcgactc agtacagtgc tgcctatgaa
    31441 accaccgaca actcgatgac caagatcatg ctctgtggcc tgaccgacaa ttcggtcgag
    31501 gaactcttgc cgctggccaa gtcatggctt cggccgccca agctgacggt taactcggcc
    31561 gcgtttgtca acgagggcta cgatcccgcc gaacgagctt acgttctggc ctgcaaggac
    31621 cacggcaagc ccgcgaagct ggaggcggac atcgcggcca atgaggaatc tcctctggtg
    31681 aacgcggcct tggttatcag agactggggc aaggccggtg ccacggcccg cctgaatcag
    31741 gtcgcggtcg aggccggaga agactttgcc gtcggccacc gggacagaat ggagggcacc
    31801 gacctgatcg tgtggattcg gacgacctcc acgacgcctg ttcacatcga gctgacgccg
    31861 gccggcgagt aacaagccga gttcgccaac cggaaggctt gctgccaggt cacaccataa
    31921 caggtcgggt agtaccgctc cggcgcttgc ggcttggcga gggcattttc acgttcgcgc
    31981 aaccggcaaa gtccatcgag gtcctcgcaa gccgccggtc agctacaggg aatcgccatg
    32041 tgctcttcga taagggcctt caagagtgat cgatggcacc gcgattccag gatgcaggca
    32101 gatgaacaaa gcagcgtgat cgtctcacca ttggccgctc gtttggccaa ttccgttatc
    32161 gtggagatct gctccttcat ctctcttagg tatcgagctc gatattgttg ccagccaatc
    32221 ggcggtctgc ctttgccgta gaaggctgcg tgcaactcgg cactcggcgc gagattcggc
    32281 agccatgcct cccatgtttc gtccgccttt ggcagcgccc gcggccggta tcgcgtgatc
    32341 agcacgcgat agccatcctc cggctcccga gggtcgtccc atcgcttggt tcgtattgcc
    32401 atgttcaggc accacacctc atgcaagacc gcgtcggatc gtgcagacac gagactctcc
    32461 tattctagcc tcgaccgcaa gggctgggca acactcgtcg cgattgcgtt cttgcccagt
    32521 cctcgataag ttaatcgtgg cgttaattca tcagatgatc tgtttttcgt ccggagacta
    32581 tgccctttgg tcattcgcct tctcattacg gtcaagacct acccgattcc ttcggcgaag
    32641 tatgatgagc tggtctgtac tgccggcgta acggaaaacg gcgacttcat ccggctctat
    32701 cccatcaatt tccgcgactt gccgtacacc cagcaatacc ggaagtacca gtggatcgag
    32761 gtcgatgcgg acaaacacac cggtcgagac gtccgaaagg agagctaccg gcccaggccc
    32821 gatactctaa aggttatcgg gaagccgata ccaactcgaa atggcgactg gtcagaacgg
    32881 gcgaaatatg tcctgaagaa aaaggcccag tcgatggagg aactccaagc gaggcaggag
    32941 gcggaccaga cctcgctggg catctttcgt ccaaagaagg tcaacgactt gatcatcgcg
    33001 cccgacgacc gcgaatggaa acctgcattc caagcggcaa tgcgacaggc tcggatatgg
    33061 gatgaacgca cggtcagcaa ggagccgcct cgaaaagtgc cgttcaagtt tcgttaccgc
    33121 ttcgagtgcg acgacccgag gtgcaggcga aaccatgaga tgatgataga ggactgggaa
    33181 gtcggcgccc tgttttggaa tcttcgcgac aaaggcgcga ccgaggcgga ggcagcccaa
    33241 ggggttcgta gcaagttcct cgacgggcta tgcgcccccg acaaggatac ccacttctat
    33301 gtgggtaccg ttctcagcca tccgaagtcg tggatcgtca ttggagtgtt ctatccgaaa
    33361 ctggagaaga cacgtgttcc cgatctgccc acgctctttg actagggcat ggaagggcct
    33421 tggcgatcaa ctcgtgactt ctgcgtcact gtcagattca caaaccgccg agcaagccga
    33481 atgcacatcg ctcgcgctca atactgcaca atcacgttga cgacctcgcc ggtcttcggc
    33541 agccggattc gctcgtgttt tggatcgacg tccgtccact ccttgccgtt gatctcgacc
    33601 ttcctgattc gtttagcatc gggcacgcga acgtagagcc agacttcctt cggcgcgttg
    33661 cgggtcggag gtcggaccgt ggccaccacc gtgttcggct cgtcgccggc gtgcatcgtg
    33721 aaggccagcg ggccgaaata cgagttgatc tcatcgacgc ggatgccctc tttcgacgcc
    33781 agccagcggc gaggcgtgcc cgaggccagc cacaggtcat cgcccgactc cagcaccagc
    33841 atgtcacgca accgattcac gaaccgggcc tcatcggggc tcttgtagaa cggcccgctg
    33901 gcccggcccc acatgcggta ttcctcggtc agggtgttaa cgtcggggta gaggcaagcg
    33961 acgaagttgt tgtagatgtt gcgaatggcc gccggcacct cgttgcgctt caggtacatc
    34021 tggatcgggt tctgcaggtt cgattgccag accatcccac cctggctgaa ccagagcctc
    34081 tcgtcggtga tcccgtgcac attgaaccgc cgctcgcccg agagcgtgag gttgtcctcc
    34141 caatcgtcca agatccaatc ggcaactacg tcgttcggct caaacattcc cagatccagc
    34201 aggatgatcg gcccgtagag cacctcacgc gtgcctgaga gcctgaaaca cggcagcacg
    34261 tcctccggct tgccgtaacg gctgtagaac tcggcctgaa gcgggccaaa ctgccggaac
    34321 cgctgccggg ctctcgtcgc gatgtacggc gaataggtgc cgtcgcgcat cttgaccacc
    34381 ggtgagatct cggcggtgcg aatcaccgag gatcgcaggt cttcgcagta tcccgcagcc
    34441 tgtcgggcga tcttttccgc gtcaggatgg cctatgtcgg ccatcgccct ggccatctcg
    34501 gtcatgcccg ccacgcagta agcattcaca ctgaaccagt agccccagtc cgcgttgtct
    34561 tcgaggtgcc cgggcggcaa cagaccgtac tcgatcggcc ggcgcccgtg gatatcctcg
    34621 tgcatggtcg ccttgcgctg ccgctcgatc cactcgaccg ccttgaacat gctcggcagc
    34681 gtcttttcaa gccatgcctt gtcacgggtg tacttgtaat gccgggccag cgaccacaac
    34741 acggtgccgt ggtcaaggtt gtactcgtgg gcggtgtagt cgtattcctt gttcactttc
    34801 gccccgtgat agacgccgtc gttggacggc gtgtacgcgc cgcggaacat ccggctgccc
    34861 tggagggcga cgaaagtgtc gagatactgc cccgcccgtt tggtgtcgcc gagagcatcg
    34921 agcagcatcg cctggaagca cgcctcgttg gcgtacacgc gatagcagta gctggcggcc
    34981 ggaaccatat acagcccgct cttggggtcc ttcgtcgtgc tgatgtgaat gtgcggcaca
    35041 acaaagcgcg acaaatagtt gaatttcggt tccggtgtgc tgaaacgcgt ggttcgctgg
    35101 atcatcgaac gccagtagtc ggccacgcgt ctgcgttcgg cgtcatagct gagcgcttcc
    35161 agcttgtcgg catcagcgcc ggcaacatcg gaaacgaacg ggattgccaa gtagaacacc
    35221 tgcgatccac cggccgtgac ctcgaaccgg caggccactg cattgactgc ttccgacgga
    35281 agcttctcca cccgctccag tttcgtctct gtcccagagg ggcaagagat gatcgcccgg
    35341 agaactcgct cggttttcgt cgggtcgtcc cccagtttag cgatgattcg ctttccagag
    35401 atctcgaacg ggctgtcagg cctggtttgg aaccagagct gcgaagctct cggctcgcgg
    35461 ccggagttgc gggcggtcag tttgatcatt agcacggccg gggtctgttc gtcgcgcgcc
    35521 gggtcgttgg ggtccagcgg accttcgagc agcgtggcga acgactcttg ttcgtattgc
    35581 aggccttttc gctcccaccg gttgatcatg atcggcaggt tctcctcggc caccgccatg
    35641 ctgcccttgt ggtcctcgcg gaaatagggc gtggctcccg tgccaatcca gaagtggatg
    35701 ctgtcaccag gccacttaag ccgcttggcc tcggcgccga acgccttcgt gcatgaccgg
    35761 gagatgaaga tgttaccgtc atatcggacg gcgaacttct gccagctcga atcggcagcc
    35821 gcgactaggt agaccatgtc gccgtgctgg cgaacccagg gatcctgcgg cgggatttcc
    35881 ttcgttgccc gctcgtatga ctgttccggc tcaagcggga tcagatcgcg gatctttccg
    35941 cctttctcga aaacggtgat gtcaaacggc ttcggatcat ccgccttggt gacatacgcg
    36001 ttaatgccgg gaacgtagat aggtcagtcg gcaaggtcgc ggttgctgaa c
//
LOCUS       DAOTPW010000018        35621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1287_length_35621_cov_5.763538, whole genome shotgun sequence.
ACCESSION   DAOTPW010000018 DAOTPW010000000
VERSION     DAOTPW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 35621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35621
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_35621_cov_5.763538"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="P5316_02820"
     CDS             complement(<1..240)
                     /locus_tag="P5316_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83918.1"
                     /translation="MTSRERILAALRLELPDRLPMCETDYWPQTLDRWRQEGFPKDAD
                     PVDYFGLDRIVCVNDLFEPSFGLPERTVEQTSEYRV"
     gene            complement(309..773)
                     /locus_tag="P5316_02825"
     CDS             complement(309..773)
                     /locus_tag="P5316_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83919.1"
                     /translation="MSSLNEKPAERSPRFMKIVVAVVLLLLAVSLGYQWWSARQSAIV
                     PPARATRDFVVTWRCLSCGHAVQGRAGPGPKKCPACGRDNMYASLRWGCPVHGAKNVA
                     FQYDEEGQPTQIKVEQADWVPAYGEQGWNIRCPTCGAAMNPVEVPNPAGDSN"
     gene            complement(770..1615)
                     /locus_tag="P5316_02830"
     CDS             complement(770..1615)
                     /locus_tag="P5316_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR83920.1"
                     /translation="MRAFTLIEILVVVSIIALLISILLPSLRRAREQAQAVVCSSNTK
                     QIITALMTYQLEAKGFIPQNLWSEYNWAPVAKKNLWFYKLYPRYLGDPNVYICPADPF
                     RDRFDFEAIKQPGNIKRANTAVPSCGYGINYVFRHFHEPESFNVERYVKRPLNTILMA
                     EVGPDHELKNVPLSGTMGQPWRDGGRLVWDDGSRSWYSGPTWLTTRHFGGINVASVAG
                     AVQRVRTAEILRKIKEEGIRRKYPDCAMGDCYFCNYHSGSDATHYNFSAARLYWWVGR
                     YPNYQ"
     gene            2089..4086
                     /locus_tag="P5316_02835"
     CDS             2089..4086
                     /locus_tag="P5316_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR83921.1"
                     /translation="MSSQETPPMVIREDVSLQEGAVLRHGLVIEADHVTIDGKGATLQ
                     GPGKPGDPATFAGVGIRLEGCKGVTIRNLKVRGFKIAFLAGDAESLLVEGNDFSDNYH
                     DPEFGWGEHERNGGMILSHVCRSVFRKNTVHRVWNALDLSESHDNLITDNNLSHASNV
                     CLKLWTSCRNIVTNNDLSYGIRIKPGEVHARDSTSVLIESGSNDNYFYRNDITHGGDG
                     IFIRVLNGWVSTGNVFVENDCSYANNNCVESWSPGNTYIRNKANHGSYGFWLGGSDQT
                     TLIGNEAAYNGLPTGLHNAPEAVFQHGGIVIVGGSSSHTIIDGNHCHHNNGGGIVFRG
                     DHETKGGKWRTHHWIVQNNRLENNRWGIWGRWGDWIHLANNTLADNSEGNYLEDVTEL
                     VEVKPDPSVTRSPIAIVRTPRRAGVGQSVVFDASQSRDPAGRPLTYRWNLGGFVTTGA
                     TVEHTFTQPGFYRVGVTVSNGVLADLGFCDLIVATEVNEEIGTEGQAACWGFELQGND
                     DGRGRILFADDPDAVVGRFSLRFTPNPYPGMYATAIFPKSRDAGWDLSKSTRAGFWLK
                     AQNPNIPGFQEPGPVLRLYGKEGEVKIQPSGGRNLLVGTPFSEARWTWMHVSIPLKGD
                     KDWSREESGKVSLERIDAVSISLDSWGGDPFTIWIDGLSFE"
     gene            4158..5426
                     /locus_tag="P5316_02840"
     CDS             4158..5426
                     /locus_tag="P5316_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRR83922.1"
                     /translation="MGVLAALAGVGPVGCAGDETPDQVTLRVANWGSPAVESSFMALE
                     RQFLREFEQLHPGVRVQVEQIPGFGQYAPKLLMMHVSGSVPDVISLDASSGAAFMDNG
                     VLRDLKPYIERDASFRLDDYFGNLVKIFRRGDKLYSIPLDFTPMMMYYNRKLFDAAGL
                     PYPRPGWTWEDFRRTAGALTIRDPNPVRPPRQYGFNFINVMPFWVLWLWTNGADVLSP
                     DGRKAGGHFDGPGSVEAVQFLTDLMLKDHVAPTLEESKAAGVDLFRSGQAAMDLKGHW
                     MMIDYRADGIDFGVVDLPTNTGSPTTVVYLTGLSITSRARHPDLAWEYVKFMTGREVQ
                     IRRVASGLAISGNRHAAAHYAGNPVEDAFIAAVEYARPPWGATVERYPFIEGLGEEML
                     EDLLYSKGSLGVAAQLRRTAALIDAALSEP"
     gene            5423..6331
                     /locus_tag="P5316_02845"
     CDS             5423..6331
                     /locus_tag="P5316_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRR83923.1"
                     /translation="MIRPYRHNWRGYLFIAPATVYLALFAFFPMILAGWMSLHRWHLL
                     KDEHPFVGLANFTAMTQDPFFRNAVKNTLAYAVFTVPLGVITSLAVAVLVSRPLRGVG
                     FFRTLYYIPAVSSQVAISMVWIWILLPGEQGLINYCLGRLNAWLGADLPTSTNFLGRP
                     GWAMAALVAMSLWIGLGPRMIIFLAGIQSIPETLYEAAALDGCGTWRRFWYVTLPLLG
                     PTTLFVTVTTTIASFQLFTPVYMITHGGPRRTTDVVLYHIYKEAWQKFEIGMASAQSY
                     VLFAMVLAAAALQLWVMRRGLTEQAE"
     gene            6328..7167
                     /locus_tag="P5316_02850"
     CDS             6328..7167
                     /locus_tag="P5316_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRR83924.1"
                     /translation="MIRRLTTIIHYLALAAIAVTTVTPFVWMVFTSLHPPSAQIPTMA
                     NLFRPAGWHFSNYSYVLTFGELPVWRFAVNSVVTTCGVVVFQLTLCSLAAYGFARLHF
                     RGRDVLFFIFLLTMMVPAQVLVVPLFAFVQRLGWLDTYAGLIIPYPYLSTAFGTFLLR
                     QFFLTVPQSLDDAARLDGCGELRILWHVILPSARPALVTVAAFAFIWTWGDFYWPLLA
                     TSSTSMRTLEVGLSVFSDAYSGTRWPLQMAAAVIVLTPVLIVFLAMQRFFVRGATMSG
                     IKG"
     gene            7221..8348
                     /locus_tag="P5316_02855"
     CDS             7221..8348
                     /locus_tag="P5316_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR83925.1"
                     /translation="MFHMTGLAGLPRLRDYRSKRVSSYDRTGGNRDWVDVEAGATRTV
                     AEIAGPGCIRHIWMTMWFPNDDYLRRVVLRFYWDCHTEPSVECPIGDFFGLGHARRKN
                     FITAVLQMSPQDGRGFNSYWPMPFREGARIEVENQGSEKHPLYYYVDYEAYDSAAAVD
                     GLGRFHVQWRRENPTVGWGDEYNHLQEHLAQDPQTADPPRWRDLMHFGGDPRSLNPSN
                     RDNYVILDAQGDGIYCGAHLDVDVFERQKNDWFGEGDDMIFIDGEPWPPSLHGTGTED
                     WYNCAFGPDQEYTAPYHGILLYSGSPEWKWKGKQSVYRYHIEDPIRFRRSIHVSIEHG
                     HANKLSNDYSSTAYYYLSKPARGGPPLLPVEQRLPRPDEPM"
     gene            8381..10531
                     /locus_tag="P5316_02860"
     CDS             8381..10531
                     /locus_tag="P5316_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRR83926.1"
                     /translation="MITVHLIFNAHIDPVWLWPWQSGLDSVLATCRSACDLLDEQADL
                     HFTQGEAWTYLQIERTDRRLFERIRRHVEAGRWHLVGGWWIQPDCNGPSGLGLRRQIE
                     LGREYFLERFGVFPRVGYNIDSFGHAACLPELLRSFGQDRYVFMRPQEHEMALPGRVF
                     RWRGRTDGPEIVTFRIARAYSTRELTVEHIRASTEQLPEGIEHTMCFAGVGDHGGGIT
                     RRQIDWCREHHDRIDGLKIMFSSPDRFFEAISDRIANLPLVTGELQMHSVGCYSVQRA
                     VKLSVRQAEHRLDQAEIVGHLDPDPEPDMGYRLREAWRNVCFTHFHDTYCGTCIPSAY
                     SQIQSQLGLSHAVADEIIQYGIRRLANRLPEDAMQRIVLLNASDEPFGGYMEYELSVE
                     DRQWEDHWQLLDEDGSVIAHQLMDVEAVVSQEWLYFRRLLFRVGIAPHAVKTLRVDRR
                     VRSACDVEHPPHLQPAWRTRIATNAGFAVDLRGTGRMFLGEAGEYPLPKLELIDDHTD
                     TWSHETDRYGLIPAARPRLRAAKLIDQGPLMASLRQRGRVGSSRIESEYRVYAGERFI
                     ELLLRIYWSEQHKVLKLTLPLAELMVRRQDGIPVGGVDRLPDGTERPVRDWTLITLAS
                     GRRLGLVFPEIYALDAWPDALRLTLLRSPLMAHHYPHPGDAPRGVFADQGLHEFRIRF
                     LAGNDVSAGDLEAQALMMHRPPIAADITLGMPPT"
     gene            complement(10545..12848)
                     /locus_tag="P5316_02865"
     CDS             complement(10545..12848)
                     /locus_tag="P5316_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRR83927.1"
                     /translation="MNETDSWHVVRTAFDPHSLGAANSIFTISNGYAGLKGNVAEDRD
                     GYYPVTLINGVYDELDMFSLIRASNEERRYLDPRYFDTAGRSPAVANLPNPLAVRLFI
                     ENREVSLGRGKVSDFRRILSLRDGIYSYSFTYKDPAGRAVRIEMTRFASMVHAHRVYM
                     RYAVTVLNGDCHVRILSGISGATRSNTTGERQYRVVSLDAAPQRCRLEAVTPARSINV
                     WMQTADVSTAAPDQPALGTLVEHDSVWSVFSFRGGSGACGRIDRHIVMGCSEDARHEA
                     TADLESELQASIDEGFDAAVETQQAAWSRIWEQCDVTIDGDDEAQRDLRFCLYHVLAA
                     APRHASGLSVPVKLLSGEYYQGNTFYDTDVYILPVYTFTQPDMARRCLQWRHIGLEPG
                     RRIARDLGYRGAKFAWQAGPYGEECLGRWWRFTHTNIHVNSDVAYALMQYWRASGDDA
                     FMASCGVDLLVETARFLASRAVYDASRDRYDIHDVAGPDEGHCESTNNFYTNYLAIRN
                     LRWAAQMLAWLHDHHPDAYAACLTRLSVSEDEPQQWNEVADKLTLLFDPQTGIYEQYD
                     GFYRLQPIPPDLLRDRKAWFVTVAPYQALNQPDVLMAMAMFRDDFPVDVRRANWLFYR
                     DKSMNFSSMSFVINSIMAADMGDLNEAYRQFRVCAGIDLHEDLTGRRDTYAGLHGTAA
                     GGAWMAAVFGFGGVRLREDGLQIRPNLPPQWKSLRFRLAYRGNMLDVAIAGHQVTLRT
                     SDDAAAPLRLLVNDQTVTLSPGDTWTS"
     gene            complement(12845..14245)
                     /locus_tag="P5316_02870"
     CDS             complement(12845..14245)
                     /locus_tag="P5316_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83928.1"
                     /translation="MMDDKLARYLDAGGAAQFLGDLARQVGADEFAVVRNLGYVYSPF
                     EIYPLAPRAALPLPRIVAFAADMDGTSTTTEPLALHALEYMVRRFTGLLTAQQWAGLD
                     REHDYPFVIGNSNYRHAEFLLNRYRQRLNREAFCSAFFEAVIWTLASMTDAQRRRDVQ
                     QNAANCGLTEMLADPEFLSAVSGGPIEPGRAAQLARPLIARFGRAFRFDDGSATVAAI
                     VDIYYMRYHWIMQLIEQGQGERLSLELLGRPGQRLIEPMAGYDVFVPLIKGWLGGEAG
                     NLYELLRSNSGLSDDSGKDIGPDGLRRLGRHFARQPAKLALVTASIAFEAHTTMKEVC
                     RVMALRVAHWPIAQALKDRVADRLADYRTVFDAFVNASDACEHRLKPHRDLYSLALAQ
                     MAVPREDYGFCVGLEDTEPGIISLRAAGIGCAVAVPNHDTSRQDYQAATVLIRHGLPE
                     VILSHNCFLAMPEVPR"
     gene            complement(14245..16479)
                     /locus_tag="P5316_02875"
     CDS             complement(14245..16479)
                     /locus_tag="P5316_02875"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR83929.1"
                     /translation="MESFNGRPMLFINGEPTTEFWCYGDPGAIEDFVSAGIRICQFHV
                     PFPSWWTGPEQYDFGPTDEKIEEFCARAGSVLLMPRVNFGYVGEEWWGDSHPDELAVG
                     LDPDGKPVDYRQVRSLPVGCWFSSGSQEWTRDAARAMHAFVTHCEERFGDRILGYQIG
                     GGISAEWFRWWYFVEDVYEDYSPAAREAFRRYLRNRYGDDMALRRAWNRPDVCLATAE
                     VPSPRKLRQPAECYFRDPAIERDVVDWLECLSEANAGQIMTLAKVAKEACRWQKLVGT
                     FYGYLWPHWNTRSPARAGHMALRRILNEKAIDFISSPYHYDNRHLGGFHHSQTVPQTI
                     ERAGKLHLDEIDTSTHLAKPPRIAGRSCGLPRNAEESCRLLRRDAASVLGTAGTGWWM
                     DLYNDRWYADLEIQAEIRRLQRLAVQSREWDCDSHAELAVVVDDRSSAWCHPTSSLNQ
                     FFTSVARQMEWSDLGFPLDTLTAWEVGRYGRARVYLFLNCWFMDGDLRREIIQRVRRP
                     GVTSVWFHGCGFIEKNRCDVANVTELVGIRMRYLPEPALPEIELIPGSDPVVEDAYTP
                     RSFGARLSDDRTDRMLDGPAAHWDAARTPRFAVDDPQARVIGRYVGGREPALAIVEKQ
                     GWRSVFCGAPMLPGWLLARLARRSGVHAYTSPGAQVFHRGPLISVYKPQAGLLRVEAP
                     AGMLIQPLMFAETQQVWRPDPRTKPCASVETPFEASETRFYVACDSSGRELPMGAAGN
                     RSAE"
     gene            complement(16521..18341)
                     /locus_tag="P5316_02880"
     CDS             complement(16521..18341)
                     /locus_tag="P5316_02880"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR83930.1"
                     /translation="MSNVFDQQAMIGGELHYGRVQRRHWGTLLDAAGELGVEVLATYI
                     IWEMHEHREGEYDFALLHEFLAEVEKRGFKLLARPGPFCYSEWRNLGIPDHAIPFGKH
                     HAEFRRKASHWIRAVMAEIRPYLNRLIVAVQADNEIDPMPHFYGEDQGFGQWLRAKYG
                     TIEKLNRAWGTCYADFTEPIPTLTPFFEDQRLRDGCRYRYDLATDYARWVVQEYRAHG
                     CDVPILLNTWPGVDAQHWHDLADLVDFYGIDPYPSNECQADYRYFRERLRLLRAVTAK
                     PYIAEFGSGIWHGMPNREYTPDHYRLTALTALAGGVRGWNWYMLHDRDNWHGAPMNER
                     GVLRPDLAEAFKQAVRWFHLFRDAPPPKASCAVAWSWHYHQLAQIRKRDVDDPLFEVL
                     HGMGLEYDFVDVDRTFEPPALLLAAGQIEQPQRLWEYVEQGGNLVLFQHLIDGCLRPD
                     GTSHPGANRLEVSLGFVCDGPVFDYRRPPGTPITARQLRITADDDQQRLWDLAADRTY
                     TTGYIEQRGRGTVMVVGCPPSRDAILAVHRYFGIAIPVLPLTPGVHAAARGDKIIVMN
                     PGEARTAKLMIDGATHNVDLPRCSGVVLQADGRGGFRCIA"
     gene            19053..19126
                     /locus_tag="P5316_02885"
     tRNA            19053..19126
                     /locus_tag="P5316_02885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19087..19089,aa:Arg,seq:ccg)
     gene            complement(19457..19636)
                     /locus_tag="P5316_02890"
     CDS             complement(19457..19636)
                     /locus_tag="P5316_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83931.1"
                     /translation="MRDLSAIQAVRVKMGGRRYRLRTDLQGSAGQIFAAAGVQPPSEV
                     TLRADLNELEGQWAM"
     gene            complement(19799..20533)
                     /locus_tag="P5316_02895"
     CDS             complement(19799..20533)
                     /locus_tag="P5316_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRR83932.1"
                     /translation="MRLISTSATTFLFCVLLSTVLPACSESRPATPSRTAPATNTTAE
                     TGHDFARWERDIAAYEATDRIHPPPKGGVLFIGSSTIRRWKTLAEDFPDHQVLNRGFG
                     GSQIIDAVHFAERIIFPYEPRMIVLRAGGNDIHAGKSPEQVFADYKAFVAKVHARLPN
                     TQIVYISNNPTPARWPEWEANKTLNTLIENYTRQRPCLKYLETSDLVLRSDGQLREEL
                     FVADRLHYNAEGYRLLAERLRPLLPK"
     gene            complement(20654..21604)
                     /locus_tag="P5316_02900"
     CDS             complement(20654..21604)
                     /locus_tag="P5316_02900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR83933.1"
                     /translation="MMRLSIIAPCYNEADNIAELRRQLEAVARELTADSRYAIDDVEF
                     VFVDDGSRDATLDKLKAWAGEDPRVRVVALSRNFGHQAALSAGIDHAIGDAVVVMDAD
                     LQHPPELILEFVRKWRAGYDLVYAYREGVRPRFGYRFINSLMKIHVPAEAADFRLMAR
                     PMVNAFRRMPEHARFIRGMISWLGFRQIGVGYTDRDRFAGERAFTVRQTAQMALNAVL
                     SFSNLPLRVASLAGLVTILCGCSYAAFILYHYLFGDASKIQPGWTALIMTVLILGGVQ
                     LLCLGIIAEYIGRIFEEVKQRPLYVVRELIGKPRDHSDGE"
     gene            complement(21601..22461)
                     /gene="panB"
                     /locus_tag="P5316_02905"
     CDS             complement(21601..22461)
                     /gene="panB"
                     /locus_tag="P5316_02905"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRR83934.1"
                     /translation="MQARSKPTLEDLRVFKQTGKKFSVLTCYDAAVAGLMEQAGVEVL
                     LVGDTAAEVVLGLPSTRDISPDFLLALTAAVRRGAPNSFVMADLPFACRVEENPDATV
                     EWARRFHVEPGTDAVKIEVTGRDAALVEAATRRGVPVVAHLGLLPQHIDPQVGYRAQG
                     RDAASAAQLISDAKRLEEAGACMLLLEAVAGEVAAEIASHTSLPVIGCVSGPGCDGTV
                     VVLHDLLGLGMGHPPRGSKQYENLAAILMRVFRSYVDDIHAGRFPTQADVPHMKAGEL
                     EKLHAVLGEA"
     gene            22675..23421
                     /locus_tag="P5316_02910"
     CDS             22675..23421
                     /locus_tag="P5316_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83935.1"
                     /translation="MGATLDALLRLQRIETQLRLVRGQIETKRRSVQAQQRRLATLER
                     QIADTHELIRQAQADSDRLELDRKAQEEHIKRLRERLNQTKTNKEYAAVLTQLNTDKA
                     DALKIEDKVLAAMGKVDDLRKREAEHRATLEKEKARMGETLKSVTELEAQLSAQIREL
                     EAQRDAAAEEVPPEALKTFERARDRHDGEAMALVEQTHPRRAEYICSGCNMSVTLETI
                     NALQSRDDVQVCHICSRILYLDSSAGVAAR"
     gene            23440..24261
                     /locus_tag="P5316_02915"
     CDS             23440..24261
                     /locus_tag="P5316_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HRR83936.1"
                     /translation="MAWTAHIDGGSRGNPGPAGAGVSIDDEKGREVFGAGFFLGHTTN
                     NQAEYAGLLRALKVLRAAGADDIRVLTDSELLAHQINGIYRVKSAELHPLYLEARKLL
                     KGFDRWDVRYVPRAQNRFADRLANQAIDARKTVLVRDRLDLARHAELDLPAVKAEGLF
                     DAKPLSDHQGDSRKGQVATSRARSAVSGIEVVVARAPRKGTCRAAMKQGDRFIFTEVT
                     PSGMCVEACAAVMEAVLAVRDAAAAGVTCDEAMSCRCPHPDCGAVFDIRQVQQVR"
     gene            24570..25304
                     /locus_tag="P5316_02920"
     CDS             24570..25304
                     /locus_tag="P5316_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HRR83937.1"
                     /translation="MIRWCIVLGTGAALLCGCVEPTGRSVVISPLPPPYYIPYVHDER
                     PVTLTTMPAEEEPDPTKAWYPDGPLFEAWGFIVIHHTATPYGSMREIDRAHRNNGWDG
                     CGYHFVIGNGTRTPAGFIEPTPRWKRQEEGAHTRLSPKMARKSGVQPTYYNEHGIGVV
                     LVGNFDEVRPSAAQMASAAELVKFLMKLCRIPEDRVIGHRDVDATDCPGRWFSMDDLK
                     QRVRALKWPTTMPVETASVRPSGRSQ"
     gene            25328..25567
                     /locus_tag="P5316_02925"
     CDS             25328..25567
                     /locus_tag="P5316_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83938.1"
                     /translation="MRRYANGRGITRWLTSLAVGASLLGALAGCRESVTLAFYEGLEN
                     LAVAVVQAFFDAITPTSSSTAGALDLMHSALPWLA"
     gene            25980..27509
                     /locus_tag="P5316_02930"
     CDS             25980..27509
                     /locus_tag="P5316_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83939.1"
                     /translation="MKVVSGDAEVVMPGVARPKESRVEGKGNESGAGRSVGRVALMVT
                     VPGVAAPPTPRLVEVCLRFGLSLTQGERVIVSGLVLDLRPGSVTMIVGPSGAGKTLLI
                     REIARRASMCQVVGDMDVPADVPVLDAVAPTRPIDEAIGLLTACGLGEPSLWMRLFDQ
                     LSEGERFRARLARAISLWRRNGRHGAILCDEFGSLLHERLAKAVAFNLRKLATRDRLA
                     VVVATSREDIERDLRPDCVIRLSDKPDLVHGSRERHPDGAGARLSFAEDLQVVPGTLR
                     DYEAFAAMHYRQRQQVGFVSAVFVMRDRRTGQALGVVVYGHPSLELALRNKVTGGRYV
                     RQAKLLNQEVRVLKRLIIHPDVRGCGLGHELVRQTLPMAGARFVECLAAMGAVNPVFE
                     KAGMRPIGVCCGSAGSEAALAWLRAAGADPLEAAFTSHVRCRPAVRRVVAREVANWYR
                     ATTGGGEQRVRKQTPTVLAQTYRQLAGSQPVYFIWAAANEDRELIEANLGDAEEAAQA
                     S"
     gene            27602..27826
                     /locus_tag="P5316_02935"
     CDS             27602..27826
                     /locus_tag="P5316_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83940.1"
                     /translation="MKVMDVLRKVVRPLLSRKVRVALATVLAAYAAEYGLGVSEEMVL
                     TILGVGAALILGIAHEDQGLKQGTGGSVRS"
     gene            27842..28375
                     /locus_tag="P5316_02940"
     CDS             27842..28375
                     /locus_tag="P5316_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83941.1"
                     /translation="MRTGREEMLPATACMTAVILGLAGCGGDARLELAAADALLAAAD
                     RMETVVAEYHAEMNEYDGTRESALIEAFVERVKSSAGDDQATAGHAAQFRLALERIRQ
                     DRAVQWRRHTAALENVAAVREVAEGLRRMGIESLTLSDEMRRYLSGWIEARKRAARPT
                     AVIQTSSAGKECDHDGE"
     gene            28362..28622
                     /locus_tag="P5316_02945"
     CDS             28362..28622
                     /locus_tag="P5316_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83942.1"
                     /translation="MTASDATRVLDDLISTLEQVTPRAGEGDAIDAVLAGKPRGTQVV
                     SVRQAPEMEVFRRELVDGLIRADTVNQVLRLLNEWVMAGLAR"
     gene            28982..29407
                     /locus_tag="P5316_02950"
     CDS             28982..29407
                     /locus_tag="P5316_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83943.1"
                     /translation="MGWEQVGVLVGVLSPLVGVPLGMITLYLRAIREQQATTTSEISR
                     RIHIIEASLDNLVKRMAECDREFTTKEEWLRESMLARQRLERLTELVTRIQAELDGER
                     GLATEIGRATEAMVELVRRLTGAGERAVERVNTTASIGK"
     gene            29808..30125
                     /locus_tag="P5316_02955"
     CDS             29808..30125
                     /locus_tag="P5316_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83944.1"
                     /translation="MGRNGGVRQIKRVRNEVLVALKMLYPAALQGDQVLRTLLAVFPQ
                     LEWDQLKKDLAYLCEKGYVQRIVADTEEDARMTSWRKRWFRLTSSGVEVADHCVEDKA
                     LEV"
     gene            complement(30133..30378)
                     /locus_tag="P5316_02960"
     CDS             complement(30133..30378)
                     /locus_tag="P5316_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRR83945.1"
                     /translation="MNLVFQPVAWTLGPMEIVIIGLVGLLIFGKRIPDLAKSLGRSIV
                     EFKKGLRETENEDPKALGNDQADILPPPEKRNNNTAH"
     gene            complement(30505..31020)
                     /locus_tag="P5316_02965"
     CDS             complement(30505..31020)
                     /locus_tag="P5316_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83946.1"
                     /translation="MKTSARLIATALIVAAASVACNQPPAVNPWADDAIGKEARTTPS
                     RDGFLAAGPEPVIRKRTLPESELRLADGAVPHYPLWWQDPFVDKGDMDDAFAWTWQDY
                     LHMPYGLGRFILNTCALPVSVVVHPPGMPMVSDGIVGKDHDAKPGNAPNPTATPADFG
                     HVAQTQPAPGS"
     gene            complement(31017..32519)
                     /gene="guaB"
                     /locus_tag="P5316_02970"
     CDS             complement(31017..32519)
                     /gene="guaB"
                     /locus_tag="P5316_02970"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRR83947.1"
                     /translation="MNRSGYPGSKITGEGITFDDVLLIPARSSVIPRVVDVRSRLTRN
                     IPINIPLISAPMDTVTESALAIALAQEGGIGIIHKNLSIEAQAREVYKVKRSESGVII
                     DPITLRPNDTVGRAREIMNLHNVSGVPITDDDSRVRGILTRRDLKFLPPASNDMVIEQ
                     VMTKTNLVTAAPDTTLEDAERILNRHKVEKLLLVHSDGRLAGLITIRDINRSRLYPTT
                     CKDSRGRLRVGAAVGVHDYERVEALIANDVDVIVVDTAHGHSDNVIETVRNIRKKHNI
                     DIIAGNIATGEAARDLIDAGADAVKVGIGPGSICTTRVVSGVGVPQVSAIMNVVAVAE
                     PAGVPVIADGGIRYSGDITKAIAAGASSVMIGSLFAGLDESPGQIVIWKGRRFKEYRG
                     MGSLGAMVSGSADRYGQGGETRADKLVPEGVEGRVPYRGQLAEFVYQLVGGVRAGMGY
                     CGTPTVEELRSKACFIRISPASVIENHPHNITITREAPNYAIDYQVEQPL"
     gene            33061..>35621
                     /gene="dnaE"
                     /locus_tag="P5316_02975"
     CDS             33061..>35621
                     /gene="dnaE"
                     /locus_tag="P5316_02975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRR83948.1"
                     /translation="MSPGKFTHLHVHTHFSLLDGAVRIKDLVEKTKLQGSSAVAMTDH
                     GNLFGVVPFYQAAVKAGVKPILGCEVYMAPGDRRERESRGIQEASYHLLLLARNRTGY
                     GHLLKLASIAYREGFYYRPRIDKEVLQAHREGLIGMSACLGGEVPQALLRGDVQAAEE
                     ITRTYLDILGEDGFFLELQDQGMDEQKVVNPAMIDLARRLGVGLVATNDVHYLDVEDA
                     EAHEVLCCISTGKRLTDDDRMRLPSNLFYLRSPAEMWETFKDVPEALENTQRIADMCN
                     VELEFGKTHAPVYRVPGGKSDREYLRELVYEGARAKYGEITDEIRERIDYELEVISSK
                     GFCSYFLIVWDFVKYARSRGIPSGARGSGCSTVVGYCLGLSAPDPLRYGLYFERFMDP
                     DRDEMPDIDIDICQEGREEVIDYVRKKYGQVAQIITFGTLQPRAAVKDVGRVLGLGFE
                     ETNAITKLIPEELKMTIDKAMEKEPDLRRLYSDNEQIRKVIDVARRLEGLSRHTGVHA
                     AGVVICDEPLENFIPLYQPPGTDQVITQFDGPSVEACGLLKMDFLGLKTLTVLERARQ
                     LVKKQQGIDLDLDRIELDDQNVYQLFVRGETKGIFQFESGGMRDVVMKMKPNRIEDLI
                     AANALYRPGPMVHIDAYVARKHGEQWSTPHPIMTEVLRETYGILVYQEQVSRLVNRLG
                     GIELKQAFRLAKAISKKKTAMIESMREPFVRGCAEKGVPRRTAEEIFEDILRFGGYAF
                     NKSHSTGYALVAYKTAYMKVYYPTEYMAALLTLEMDSTDKVVEYIEECKRMGIRVSAP
                     DVNLSDNDFTVIRSERGKGEIRFGLQAIKGVGEKAVSAIRAARSEGGPFKSIFDF"
BASE COUNT         6776 a        10467 c        11599 g         6779 t
ORIGIN      
        1 aactcgatac tcggatgtct gctcgaccgt acgttcaggc agcccgaatg agggctcgaa
       61 gaggtcgttg acacagacga tgcggtccaa tccgaagtag tcaaccgggt cggcgtcttt
      121 tgggaaaccc tcctgtcgcc agcgatccag ggtttgcggc cagtagtcgg tctcgcacat
      181 cgggagccgg tcagggagtt cgagccgcaa ggccgcgaga attcgttcac gtgaagtcat
      241 ggtttcagga gtgtacccca ggcgaatgtc ggcgccgaga cggtaagtct tcggctacga
      301 gcttaccgtc agtttgaatc cccagccggg ttggggactt cgacggggtt catggcggct
      361 ccgcaggtcg ggcagcggat gttccagccc tgttcgccgt aggccggcac ccaatccgct
      421 tgttccacct tgatctgtgt cggctggccc tcctcatcgt actgaaaagc gacgttcttg
      481 gcgccgtgaa cagggcaacc ccatcgcaag ctggcgtaca tgttgtccct gccgcaagca
      541 gggcacttct tcggccccgg gcccgctcga ccctggacgg catggccgca agacaaacat
      601 cgccaggtca ccacgaagtc tcgagttgcc cgggcgggtg gcacgattgc cgattgccgc
      661 gcagaccacc attggtatcc gagcgagacc gccaacaaca ggaggacaac ggccacgacg
      721 atcttcatga atcgtggaga acgctctgcg ggcttttcgt tcaagctgct cattggtaat
      781 tcgggtagcg accgacccac cagtaaagcc tcgccgccga gaagttgtag tgggtcgcgt
      841 cggagccgct gtggtagttg cagaagtagc agtctcccat ggcgcagtcg gggtatttgc
      901 gccggatgcc ttcctccttg attttccgaa ggatttcagc ggttcgcacg cgctgcacgg
      961 cgccggcgac gctggccacg ttgattccgc cgaagtggcg ggtggtcagc caggtgggac
     1021 cggaatacca cgagcgagac ccgtcgtccc agacgagtcg gccgccgtcc cgccagggct
     1081 ggcccattgt cccgctcagc ggcacgtttt tcagttcgtg gtcgggaccg acctcggcca
     1141 tcaggatcgt gttcaacggg cgcttgacgt agcgttccac gttgaaggac tcgggttcgt
     1201 ggaaatgccg gaatacgtaa ttgattccat agccacatga gggcacagcg gtgtttgccc
     1261 gcttgatgtt tcccggctgc ttgatggcct caaagtcaaa ccgatcgcgg aacgggtcgg
     1321 ccggacagat gtacacgttg gggtcaccga ggtatcgggg gtagagcttg tagaaccaga
     1381 ggtttttctt ggccacggga gcccagttgt actcgctcca gaggttctgc ggtatgaatc
     1441 ctttggcttc gagctggtag gtcatcaggg ccgtgatgat ctgcttcgtg ttagagctgc
     1501 acacgacggc ctgggcttgt tcccgggccc gccggaggga aggcagcagg atcgagatca
     1561 gcaaagcgat gatcgaaacg accaccagga tctcgatcag ggtaaatgcc cgcatcgccg
     1621 accgccttgg ggtggtcaag ctcccgtggt cggccggtgc gtggtgagcc gccgtgccgc
     1681 cgggaaccca gcatcgcgcg taaccggaac agtaatagga atcgagaacc ataggcatcg
     1741 gcccgtgagc gccgggcaag accataccag acacaacgaa cagcgactgc ctttaagata
     1801 gcgaatttgc ctgacgcccg caaggacttt ccctttcgcg ggccgtgatt atcatggtgg
     1861 aacggctcgg gcacggcacg tcaggctact ggtgccgttt tcggtcgatg agttgcaggc
     1921 cgacggacag tctgcttcgc cgatgcgtcc gcgtcgactg cgaaggccga aaaccgccga
     1981 cagcacccgc cgaatggccg gaagtcgtca accggacagg ccgcctgcga atcttgggag
     2041 gtatgtatga gctgcctggt tctccaactt ctcttaggag ttgtcgccat gtcctctcag
     2101 gaaacgccac cgatggtcat ccgagaagac gtttcgctcc aggaaggcgc cgtcctgcgg
     2161 cacggcctgg tcattgaggc cgaccacgtt accatcgacg gcaagggggc gacgcttcag
     2221 ggacccggca agccgggcga cccggccact ttcgccggcg tcggcatcag actcgaaggg
     2281 tgcaagggcg ttaccatccg caacctgaaa gtccgcggct ttaagattgc ctttctggcc
     2341 ggcgatgcgg agagtctgct cgtggaaggc aacgattttt cggacaacta tcacgacccg
     2401 gagttcggct ggggggagca tgagcgcaat ggggggatga tactgtccca cgtttgccgc
     2461 agcgtgtttc gcaagaacac ggtccatcgc gtgtggaacg cgctggacct cagcgagagc
     2521 catgacaacc tgatcaccga caataacctg tcccatgcct cgaacgtgtg cctcaagctt
     2581 tggacctcct gccgcaacat cgtgaccaac aacgatctct cctacggcat ccgcatcaag
     2641 ccgggcgaag tccatgctcg cgactccacc agcgtgctga tcgaaagcgg ctcgaacgac
     2701 aactactttt accgcaacga tatcacgcac ggcggcgacg gcattttcat tcgggtgctc
     2761 aacggatggg tgtccaccgg caatgtgttc gttgaaaatg actgttcgta cgccaacaat
     2821 aactgcgtcg aatcctggag cccgggcaac acctacattc gcaacaaggc caatcacggc
     2881 agctacggtt tctggctcgg cggtagcgac cagacgaccc tcattggaaa cgaagccgcc
     2941 tacaacggac tgcccaccgg gttgcacaac gctcccgagg ccgtcttcca gcacggcggg
     3001 atcgtcattg tcggcggctc ttccagccac acgatcatcg acggcaacca ctgccatcac
     3061 aacaacggcg gcggcatcgt tttccgcggc gaccacgaga ccaagggggg caagtggcgg
     3121 acccaccatt ggatcgttca gaacaaccgg ctggagaaca accgctgggg gatctggggc
     3181 cgatgggggg actggattca cctcgccaac aacactttag ccgacaacag cgagggcaat
     3241 tacctcgagg acgtcaccga gctggtcgaa gtcaagcctg acccgtcggt gacgagatcc
     3301 ccgatcgcca ttgtgcggac gcctcggcgg gccggtgttg ggcagtcggt cgtcttcgac
     3361 gcctcgcaaa gccgcgatcc ggccggccga ccgctgacct accgctggaa cctcggcggt
     3421 ttcgtgacca ctggggcgac ggtcgagcac actttcaccc agccgggttt ctaccgagtg
     3481 ggcgtgacgg tgagtaacgg cgtactggcc gatctcggtt tctgcgatct cattgtggcc
     3541 acagaagtga acgaggagat cggtaccgaa ggtcaggccg cctgctgggg cttcgagctg
     3601 caaggcaacg acgacggccg cgggaggatc ctctttgccg atgaccccga cgcggttgtg
     3661 ggccgattct ctctccgctt tactcccaac ccgtaccccg gcatgtatgc gaccgccatt
     3721 ttcccaaaaa gtcgggacgc cggatgggac ttatccaaaa gcacccgagc cggcttctgg
     3781 ctgaaggccc agaaccccaa tattcccggc ttccaggagc ctggtcccgt ccttcgtctt
     3841 tatggaaagg aaggcgaagt gaagattcag ccctccggcg ggcggaacct gctggtgggg
     3901 accccgttca gtgaagcccg ctggacctgg atgcacgtca gcatcccgct caaaggcgac
     3961 aaggattggt cgcgggaaga atctggtaag gtcagcctcg aacggatcga tgccgtgagc
     4021 atttcgctgg actcttgggg cggcgacccg ttcacgatct ggatcgacgg actgagcttt
     4081 gaatgaaaaa ccgacgcgcc gccggccgcg ggagccgtat gaggagaaga acatcgacat
     4141 ttctcggaag ggttgctttg ggagttctgg ctgcgcttgc gggcgtgggg ccggtcggtt
     4201 gcgccggcga tgagacgccg gaccaggtca cccttcgcgt ggccaactgg ggcagcccgg
     4261 ccgtcgagtc ctccttcatg gcgctcgagc gacaattcct tcgcgagttc gagcagcttc
     4321 atccgggtgt ccgggtccag gtcgagcaga tcccgggctt tggccaatac gcaccgaaac
     4381 tgctgatgat gcacgtgagc ggcagcgttc ccgacgtcat ctcgctcgac gcatcgtcag
     4441 gggcggcatt catggacaac ggggtgctcc gcgacctcaa accatatatc gagcgggacg
     4501 cgagttttcg cctcgatgac tacttcggca atctggtgaa gattttccgg cgcggtgaca
     4561 agctctactc tattccgctc gattttactc ccatgatgat gtactacaac cgcaagctct
     4621 tcgacgcggc cggcttgccc tatcctcgac cgggctggac ctgggaggac ttccggcgca
     4681 cagccggcgc gctgaccatc cgggatccca atccggtcag gccgccgcgt cagtatggtt
     4741 tcaacttcat caacgtgatg ccgttttggg ttctctggct atggaccaac ggagccgacg
     4801 tgttgagtcc cgatggtcgc aaggccggcg gtcactttga cgggcccggc tccgtggaag
     4861 ccgtgcaatt cctcacggat ctgatgctca aggaccacgt cgccccgacc ctggaggaaa
     4921 gcaaggccgc cggtgtcgac ctcttccgca gcgggcaggc ggccatggat ctcaagggcc
     4981 attggatgat gatcgactac cgcgccgacg ggattgactt tggggtggtg gatctgccga
     5041 ccaatacggg ctcgccgaca accgtggtgt accttaccgg cctatcgatt accagtaggg
     5101 caaggcaccc tgatctggcg tgggagtatg tcaagttcat gaccggccgg gaggttcaga
     5161 tccgccgcgt ggcctcgggc ctggccattt ccggcaaccg ccacgctgcc gcgcactacg
     5221 ccggcaaccc cgtggaagac gctttcatcg cggcggtgga gtacgcccgc cctccgtggg
     5281 gtgcgacggt cgaacgctat cctttcattg aaggcctggg cgaggaaatg cttgaggatc
     5341 tcctgtattc caagggttcg ttgggcgttg cggcgcagct tcgtcggact gcggcactca
     5401 tcgatgcggc actgagcgag ccatgattcg tccttaccgg cacaactggc gcggctacct
     5461 gttcatcgcc ccggcgaccg tctacctggc attgttcgcc tttttcccaa tgattcttgc
     5521 cgggtggatg tctttgcacc gctggcacct gctcaaggac gaacacccgt tcgtgggact
     5581 ggccaacttc acggccatga cccaagatcc gttcttccgc aacgcagtaa agaacactct
     5641 cgcatatgcg gttttcacgg tcccgctggg ggtgatcacg tcgcttgcgg tggcggtact
     5701 ggtgtctcgg ccgctacgag gcgtgggctt tttccgcacg ttgtactaca taccggccgt
     5761 cagctcgcag gtagccatct ccatggtctg gatctggatt ctgctgcccg gcgagcaggg
     5821 gctgatcaat tactgcctcg gccgactgaa cgcctggctg ggggcggatc tgcctacgag
     5881 caccaacttc ctggggcggc ccgggtgggc catggctgcc ctggtagcca tgagcctgtg
     5941 gatcggccta gggccacgga tgatcatctt ccttgcaggg attcaatcga ttcccgagac
     6001 tctgtatgag gccgctgccc tcgacggctg cggtacgtgg cggcgattct ggtacgtcac
     6061 cctgccgctc cttggtccga cgacgctctt tgtcaccgtc accacgacca ttgcatcctt
     6121 ccagcttttc acccctgtct acatgataac gcacggcggg ccgcgccgga cgacggacgt
     6181 tgtgctttat cacatttaca aggaggcgtg gcagaaattc gagatcggca tggccagtgc
     6241 acagtcctac gtgctcttcg ccatggtcct ggcggcggcg gccttgcagc tttgggtcat
     6301 gcggcgggga ctcacggagc aggcagaatg atccggcggc ttaccactat cattcactat
     6361 ctggctctcg cggccatcgc cgtcacgacg gtcaccccgt tcgtatggat ggttttcacc
     6421 tcgctgcacc cgccgagcgc ccagattccc acgatggcca acctgtttcg tcctgcgggc
     6481 tggcacttca gcaattactc ctatgtcctg acattcggcg agctgcccgt atggcgtttc
     6541 gcggtgaaca gcgtggtcac cacctgcggg gtggtcgtct tccaattgac cttgtgcagt
     6601 ctggccgctt acggattcgc ccgcctgcac tttcgcggcc gtgacgtttt gttcttcatt
     6661 ttcctgctga ccatgatggt tccggctcag gtgctggtgg ttccgctgtt tgcgttcgtg
     6721 cagcggctgg gctggctcga cacctatgcg gggctgatta ttccctaccc ctacttgagc
     6781 acggcattcg gcacgttcct gttgcgacag ttttttctga ccgtccccca gagtctggac
     6841 gatgccgctc ggcttgacgg ctgcggcgag ctgcgcattc tctggcacgt gatcctgccg
     6901 tcggcccggc cggccttggt cacagtggcg gccttcgcct tcatctggac ctggggcgac
     6961 ttctattggc ccctcctggc taccagttcg accagcatgc ggacgcttga ggtggggctg
     7021 tcggtcttca gcgacgctta cagcggtacc cgctggcccc tgcagatggc cgccgcagtg
     7081 atcgtgttga cgccggtgtt gatcgtgttc ctggccatgc agcgtttctt cgttcgcggc
     7141 gcgacgatga gcgggatcaa gggatgagct tggggtccgc gacagattcg caagacgatc
     7201 tgccattgaa ggagggtacc atgttccaca tgacggggct ggccggattg ccgcgcctgc
     7261 gagattaccg cagcaaacgg gtcagcagtt acgaccgcac gggcggaaac cgcgattggg
     7321 tcgacgttga agccggggca acacgcacgg tcgccgagat cgccgggcct ggctgcatcc
     7381 gccacatctg gatgaccatg tggtttccca acgatgatta ccttagaagg gtcgtgctga
     7441 ggttctattg ggattgtcac accgagccca gcgtcgagtg tcccatcggc gatttcttcg
     7501 gcctgggtca tgcgcgccga aagaacttca tcaccgccgt tctgcaaatg agtccacagg
     7561 atggtcgcgg tttcaactcg tactggccga tgccctttcg cgagggcgcg cggatcgagg
     7621 tggagaacca gggcagcgaa aaacacccct tgtattacta cgtggactac gaagcctacg
     7681 attccgccgc ggccgtcgat ggtttgggtc gtttccacgt ccagtggcgg cgggagaacc
     7741 ccaccgtcgg ttggggcgac gagtacaacc acctgcagga acacctggct caggatccgc
     7801 agaccgccga tccgccgcga tggcgtgacc tgatgcactt cggaggcgat cctcgcagtc
     7861 tcaatccgtc caatcgcgac aactatgtca tcctcgacgc ccagggggac ggcatatact
     7921 gcggcgctca ccttgatgtg gacgtcttcg agcgacagaa gaacgattgg ttcggcgagg
     7981 gcgacgacat gatcttcatt gacggtgagc cctggcctcc ctcgctgcat ggcacgggca
     8041 ccgaggactg gtacaactgc gccttcgggc cggaccagga atacacggcg ccctaccacg
     8101 gcatattgct ttacagcggc tcgccggaat ggaagtggaa ggggaagcaa tcggtatacc
     8161 ggtatcacat agaggatccg attcgcttcc gccgttcgat ccatgtctcg atcgagcacg
     8221 gccacgcgaa caagctgagc aacgactaca gcagtacggc gtattactac ctgtcgaagc
     8281 cggcccgtgg ggggccgccg cttttgccgg tcgagcagcg cctgccgagg cccgatgagc
     8341 cgatgtgacc gcatgccggc ctttacatgg ggcacaaacc gtgatcaccg ttcacttgat
     8401 ctttaacgcc cacatcgacc ccgtatggct ctggccgtgg cagtcggggc ttgacagcgt
     8461 tctggctacc tgccggtcgg catgtgatct gctcgacgag caggcggacc tgcacttcac
     8521 ccaaggcgag gcttggacgt atcttcagat cgaacggact gatcggcggt tgtttgaacg
     8581 catccgccga cacgtcgagg ccgggcggtg gcacttggtc ggcggatggt ggattcagcc
     8641 cgactgcaac ggtccgtccg ggctcggctt gcgacgccag atcgaactcg ggcgggaata
     8701 cttcctcgaa cggttcggcg tcttcccgcg agtcggctac aacatcgaca gttttggaca
     8761 cgccgcatgc ctgccggaac tgctgcgctc tttcgggcag gaccgctatg tgttcatgag
     8821 gccgcaggaa cacgagatgg ccttgcccgg ccgggtcttc cgctggcgag gccgcaccga
     8881 cggtcccgag atcgtcacct ttcggatcgc gcgggcatac tcgacacgcg aactgaccgt
     8941 cgagcacatc cgggcatcca ccgagcagtt gccggaaggg atcgagcaca ccatgtgctt
     9001 tgccggcgtc ggcgaccacg ggggcgggat cacacgccga cagatcgact ggtgccgcga
     9061 gcaccacgac cgcatcgacg gcctgaagat catgttctcg tcgccggaca ggttcttcga
     9121 ggcgatcagc gaccgcattg cgaatcttcc gctggtcacc ggtgaattgc agatgcacag
     9181 cgtggggtgc tacagcgtcc agcgggcggt caaactgagc gtccggcagg cggagcatcg
     9241 tctggaccag gctgagattg tcggacactt ggacccggac cccgaacccg acatgggata
     9301 ccgcttacgt gaagcgtggc ggaacgtctg cttcacccat ttccacgaca cgtactgcgg
     9361 cacctgcatc cccagcgcgt acagccaaat ccagtcgcaa ctgggcctta gccatgcggt
     9421 tgccgacgag atcattcagt acggtatccg gcggctggcg aatcggctgc ccgaagatgc
     9481 gatgcagcga atcgtgctcc tgaacgcatc ggatgaaccc ttcggcggtt acatggagta
     9541 tgagctgtcg gtagaagacc gccagtggga ggaccattgg caactgctcg acgaagacgg
     9601 ctcggtgatt gcccatcaac taatggatgt cgaggcagtc gtgtctcaag aatggctcta
     9661 ttttcgtcgg ttgctgtttc gtgtgggcat cgccccgcac gcggtcaaga cgttgcgggt
     9721 tgaccgacgg gttcgatcgg cctgcgatgt cgagcacccg ccccatctgc agccggcttg
     9781 gcggactcgc atcgccacta acgccggatt tgccgtcgat cttcgaggga caggacgtat
     9841 gttcctcggc gaagccggcg agtacccttt gcccaagctc gaactcatcg acgatcacac
     9901 cgacacctgg agccacgaga cggatcggta cggcctgatc cccgcagcgc gcccgcgatt
     9961 gcgggctgcc aagctcatcg atcaaggccc gctgatggcc tcgcttcgtc aacggggccg
    10021 ggtcggtagc agccggatcg agtcggaata tcgcgtctat gccggtgaac gattcattga
    10081 actgctgctg cggatttact ggtctgagca acacaaggtg ctgaaactca ccctgccgtt
    10141 ggcggaactg atggtccgac ggcaggacgg gatcccggtc ggcggagtgg atcgtctgcc
    10201 tgatggtacc gagcgcccgg tgcgtgactg gaccttgatc acactggcaa gcggccgccg
    10261 gctggggctt gtcttccccg aaatctatgc cttagatgcc tggcccgacg cgctgcggct
    10321 taccctgctt cgcagtccgc tcatggccca tcattaccca caccccgggg acgcgccgcg
    10381 aggcgtattt gcggaccaag gtcttcacga gtttcgcatt cgcttcctcg ccggtaacga
    10441 tgtgagtgca ggcgaccttg aggctcaggc cttgatgatg caccggccac cgatcgccgc
    10501 ggacatcacg ctgggaatgc cgccgacata gcagcgttgc gaagtcagct cgtccaggtg
    10561 tcgcccggtg acagagtgac cgtctgatca ttaaccagca agcgaagtgg cgcagccgca
    10621 tcgtcgcttg tacgcagcgt gacctggtga cccgcgatcg ccacgtcgag catgtttccg
    10681 cgataagcga gcctgaaccg caggcttttc cactgcggcg gcagattggg gcgaatctgc
    10741 aagccgtcct ctctcagccg aacgcccccg aaaccgaaca ccgccgccat ccaggccccc
    10801 cccgcagccg tgccgtgaag gcccgcgtaa gtatcccttc gtccggtcag atcctcgtgc
    10861 agatcgatac ccgcgcagac gcggaattgc cgataagcct cattcaaatc acccatgtcc
    10921 gcggccatga tcgagttgat cacgaagctc atggacgaga agttcatgct cttgtccctg
    10981 taaaacaacc agttggcgcg gcggacatcg acgggaaaat cgtcgcggaa catagccatg
    11041 gccatgagta cgtcgggctg gttcaatgcc tggtagggag ccacggtgac gaaccacgcc
    11101 ttgcgatctc gaaggagatc gggagggatc ggttgaagcc gatagaaacc gtcatactgc
    11161 tcgtagatgc ccgtctgcgg atcgaagagt aacgtgagct tgtcggccac ctcgttccac
    11221 tgctgcggct cgtcctcgga gaccgacagc cgcgtcaggc atgcggcgta agcgtcgggg
    11281 tggtgatcat ggagccacgc cagcatctgg gccgcccacc tgaggtttcg aatggccagg
    11341 taattcgtgt agaaattgtt ggtactctca cagtgacctt cgtcggggcc ggccacgtca
    11401 tggatatcgt agcggtcgcg gcttgcgtca taaaccgcac gtgaagccag gaatctcgcc
    11461 gtctcgacca acaggtccac gccgcagctt gccatgaatg cgtcgtcgcc gcttgctcgc
    11521 cagtattgca tcaaggcgta agcgacgtcg gaattcacat ggatgttcgt gtgcgtgaac
    11581 cgccaccagc ggcccaggca ttcctcgccg taggggccgg cctgccaggc gaatttggcc
    11641 ccacgataac ccaggtcacg agcgatgcgc cgccccggtt ccaggccgat atgccgccac
    11701 tggaggcacc ggcgggccat gtcgggctgc gtgaaggtgt agaccggcag gatgtacacg
    11761 tcggtgtcgt agaacgtgtt gccctggtaa tactcgccgg acagaagctt gacgggcacg
    11821 ctgagcccgc ttgcatgtcg tggggcagcg gccaggacgt gatacagaca gaaacgcagg
    11881 tcgcgctgag cctcatcgtc gccgtcgatc gtgacgtcgc attgctccca tatcctgctc
    11941 caggccgcct gctgcgtctc gacagcggcg tcgaagccct cgtcaatcga agcctgcaac
    12001 tcgctctcga gatccgccgt tgcttcgtgc ctcgcatcct cactgcagcc catcacaatg
    12061 tggcgatcga ttcggccgca cgcccctgat cccccacgga agctgaagac tgaccatacg
    12121 ctatcatgct cgaccagggt tccaagggcg ggttgatccg gcgcggccgt ggacacgtcg
    12181 gcggtttgca tccacacgtt aatgctccgg gcgggcgtga cggcttcgag gcggcagcgc
    12241 tgcggcgcgg cgtcgagcga cacgacgcgg tactgccgct cgccggtggt gttggagcga
    12301 gtcgcaccgc tgattccgct caggatccgg acgtggcaat cgccgttgag tacggtaacg
    12361 gcgtatcgca tgtacactcg atgagcatgc accatggatg cgaaacgcgt catttcgata
    12421 cggacggcgc ggccggcggg atctttgtaa gtgaaggagt acgaatagat tccatcccgc
    12481 aatgagagga tccggcggaa gtcgctgacc ttgccacgac cgagcgagac ctcgcggttc
    12541 tcgataaaca gccggactgc cagggggttc ggcaggtttg ccaccgcggg gctccggccg
    12601 gccgtatcga agtaacgcgg atccaggtaa cggcgttcct cgttcgaggc ccggatcagg
    12661 ctgaacatgt ccaactcgtc atagacgccg ttgatcagcg tcacggggta atacccgtcg
    12721 cgatcctcgg cgacgttgcc tttcagaccc gcgtagccat tggagatggt gaagatgctg
    12781 ttggcggctc ccaggctgtg cggatcaaag gcggtccgca cgacgtgcca gctatcggtc
    12841 tcgttcatcg cggcacctcg ggcatggcaa gaaagcagtt gtgcgacaga atcacctcgg
    12901 gcaggccgtg ccggatgagg acggttgccg cctggtagtc ctgccgtgac gtgtcgtgat
    12961 tgggcacggc caccgcgcag ccgatccccg ccgcccgaag cgaaatgatt ccgggttcgg
    13021 tgtcttccag ccccacgcag aagccgtaat cctcgcgggg gacggccatc tgggccaagg
    13081 ccaggctgta caggtcgcgg tgcggtttga gccgatgctc gcacgcgtcc gacgcattga
    13141 cgaacgcgtc gaacaccgtc cgatagtcgg ccaagcgatc tgccacgcgg tctttcagtg
    13201 cctgcgcgat gggccagtgg gccactcgca gggccatgac ccggcatact tccttcatgg
    13261 ttgtatgcgc ttcaaatgca attgaggcgg tcacgagggc cagcttggcc ggttggcggg
    13321 caaaatggcg gcccagacgc cgcaaaccat cgggaccgat gtccttcccc gaatcatcgc
    13381 tcagtcccga gtttgagcga agcagttcat agaggttccc ggcctcgccg cccaaccagc
    13441 ccttgatcag cggcacaaac acatcgtagc ctgccatggg ttcgatcagc cgttgcccgg
    13501 gcctgccgag cagttccagg ctgagtcgtt ctccctggcc ctgttcgatg agttgcatga
    13561 tccagtgata ccgcatgtag tagatgtcga cgatggccgc gacggtcgca cttccatcgt
    13621 cgaaccgaaa cgcccgaccg aatctggcaa tcagcggtcg cgcgagctgc gccgccctgc
    13681 ctggttcgat cggcccgccg gagacggcac tgagaaactc cggatccgcc agcatttcgg
    13741 tcaacccgca attagccgcg ttctgctgca cgtcgcgccg tcgttgagca tccgtcatgc
    13801 tcgcgagcgt ccagatgacc gcctcgaaaa acgcactgca gaaggcctcc cgattcaatc
    13861 gctggcggta ccggttgagc agaaactcgg cgtggcggta attgctgttc ccgatcacaa
    13921 aggggtagtc atgctcccgg tccagccccg cccactgctg agcggtcagc aggccggtga
    13981 accgccgaac catgtattcg agcgcgtgca gggcaagcgg ctcggtggtc gtgctcgtgc
    14041 cgtccatgtc cgcggcgaag gccacgatcc tgggcaacgg cagggccgca cggggggcca
    14101 gcggatagat ctcgaacggg ctgtacacgt agccaaggtt gcggacgacg gcgaattcgt
    14161 ccgcgcccac ctgtctggcc aggtcgccca ggaactgcgc ggccccgccg gcatccagat
    14221 atcgagccaa cttgtcgtcc atcatcactc agcagaccgg ttgccggccg ctcccattgg
    14281 caactccctg ccggaagagt cgcacgcgac atagaacctg gtttcgcttg cctcgaacgg
    14341 cgtttccacc gaggcacagg gcttcgtgcg cggatcgggt cgccatacct gttgagtctc
    14401 ggcgaacatc agcggttgaa tcagcatccc ggcaggcgcc tcaaccctga gcaaccctgc
    14461 ttgtggcttg tacaccgaaa tcagcggtcc gcgatgaaac acctgcgcgc ccggcgacgt
    14521 gtaagcgtga acgccgcttc gcctggccag gcgggcgagc aaccagccgg gcagcatcgg
    14581 cgctccgcag aagacgcttc gccagccctg cttttccacg atcgccaacg ccggttccct
    14641 cccgccgaca tagcgtccga tgaccctcgc ctgcgggtcg tccacggcga atctcggcgt
    14701 ccgggcggca tcccagtggg ccgccggacc gtccagcatg cggtcagtgc ggtcatcgct
    14761 cagccttgct ccgaaagaac ggggggtata tgcatcttca acaacggggt cgctgcccgg
    14821 aatcaactcg atctccggca gtgccggctc cggcaggtaa cgcatacgga tgcccaccaa
    14881 ttcggtgacg tttgccacgt cgcaccgatt cttctcgatg aacccgcagc catggaacca
    14941 aacggatgtc actcctggcc gacgcacgcg ttgtatgatc tcccgtcgca gatcgccgtc
    15001 cataaaccag cagttcagga acagatacac gcgggcccgt ccataccggc cgacctccca
    15061 ggccgtgagc gtatccaggg ggaaacccag gtcgctccac tccatttgac gcgccacgga
    15121 tgtgaagaac tgattcagcg aacttgtcgg atgacaccag gcagagctgc gatcatccac
    15181 aaccacggcc agctcggcat gagaatcgca gtcccactcg cggctctgga cagcgaggcg
    15241 ttgcaaccgt cggatctcgg cctggatttc gaggtcagcg taccagcggt cgttgtacag
    15301 gtccatccac cacccggtcc ccgccgtgcc cagtacactg gccgcgtccc ggcgaaggag
    15361 tcgacagctc tcctccgcgt tccgcggcaa gccgcaggat ctgccggcaa ttctgggcgg
    15421 cttggccaga tgcgtcgagg tgtcgatctc gtcgagatgc agcttgcccg cccgttcgat
    15481 tgtctgcggc acggtctggc tgtgatgaaa tccgcccagg tggcgattgt catagtggta
    15541 gggcgagctg atgaagtcga ttgccttctc gttgagaatc cgccgcaggg ccatgtgtcc
    15601 ggcacgcgcc gggctgcgcg tgttccagtg cggccagaga tagccgtaga acgtgcccac
    15661 cagcttctgc caccggcagg cctccttggc tactttagcc agcgtcatga tctgcccggc
    15721 gttcgcctcg ctcagacact ccagccagtc gacgacgtcg cgttcgatcg ccggatcgcg
    15781 gaaatagcac tccgcgggtt gtctcagctt tctcggcgac ggcacttcgg ccgttgccag
    15841 gcagacgtca ggccggttcc acgccctgcg caaagccata tcatcgccgt atcggttccg
    15901 caggtatcgc cggaacgcct cgcgggccgc cggcgagtaa tcctcataaa cgtcctcgac
    15961 aaaataccac cagcgaaacc actccgccga gataccaccg ccgatctggt atcccagtat
    16021 gcggtcgccg aagcgttctt cacaatgcgt tacgaacgcg tgcatggctc gcgctgcatc
    16081 ccgcgtccac tcctgcgagc cggatgagaa ccagcagccc accggaaggc ttcgcacctg
    16141 ccggtaatcc acgggtttac cgtccgggtc caggcctacc gccagctcgt cgggatgact
    16201 gtctccccac cattcctcgc cgacgtaacc gaagttgact ctcggcatca gcagaacgga
    16261 ccccgcccgc gcgcagaatt cctcgatctt ctcgtcggtc ggcccgaagt catattgttc
    16321 aggcccggtc caccatgatg ggaacggcac gtgaaactgg cagatgcgta taccggcgct
    16381 gacgaaatcc tcgatggccc cggggtcgcc ataacaccag aactcggtcg tgggctcgcc
    16441 gttgatgaaa agcatcggcc ggccgttgaa cgattccact cgcgccgtca gacccgtgcg
    16501 tgcgctcggt ctttccgttc tcaggcgatg caccggaagc ccccgcggcc gtcggcctgc
    16561 agcacgaccc ccgagcagcg tggaaggtcc acgttgtggg tggcgccgtc gatcatcagc
    16621 ttggccgtcc gcgcctcacc cgggttcatc acgatgatct tgtcaccccg cgcggcggca
    16681 tgtacgcccg gcgtcaaggg cagtaccggg atcgcgatgc cgaagtatcg atgaacggca
    16741 agaatcgcgt ctcggcttgg cgggcaccct acgaccatga ccgtcccacg cccgcgctgc
    16801 tcgatgtatc ctgttgtgta agtgcggtcg gcggccagat cccacaaccg ctgctggtcg
    16861 tcatcagccg tgatccgcag ttgtcgagcc gtgatcgggg tgcctggtgg cctgcgataa
    16921 tcaaagacgg gcccatcgca aacgaagccc agcgagacct ccagtcggtt ggcccccgga
    16981 tgacttgtcc cgtcaggccg taagcacccg tcgatcaaat gttgaaacag caccaggttc
    17041 ccaccttgct ccacatattc ccagagacgt tggggctgct cgatctgccc ggccgccagc
    17101 agcagcgccg gtggttcaaa cgtgcgatct acatccacaa aatcgtattc cagccccatt
    17161 ccgtgaagca cctcgaacaa gggatcgtcc acgtcacgtt tgcgaatctg cgcgagctgg
    17221 tggtaatgcc aggaccatgc gaccgcgcag gatgcctttg gcgggggcgc gtccctgaac
    17281 aaatgaaacc agcgcaccgc ctgcttgaag gcctccgcca gatcgggccg cagcacgccg
    17341 cgctcgttca tgggtgctcc gtgccagttg tcccgatcgt gcaacatgta ccagttccag
    17401 ccacggacgc cgccggccag agccgtcagg gcggtgagcc ggtaatggtc cggcgtgtat
    17461 tcacggttgg gcatgccatg ccagatacca ctgccaaatt cggcgatgta gggcttggcg
    17521 gtcacggccc gcaacaggcg cagccgttca cgaaagtagc ggtaatccgc ctgacactcg
    17581 ttggaagggt agggatcgat cccgtagaaa tccaccaagt cagccagatc atgccagtgc
    17641 tgggcgtcga cgcccggcca agtgttcagc agtatgggca catcgcaacc atgagcccgg
    17701 tactcttgga cgacccatcg tgcgtagtcc gtggccaaat cgtatcggta gcgacaaccg
    17761 tcccgcagcc gctgatcttc gaagaatggt gtcaatgtcg ggatcggttc ggtgaaatcg
    17821 gcgtaacagg tgccccacgc gcggttcagt ttttcgatgg taccgtattt cgcccgcagc
    17881 cactgcccga aaccctgatc ctccccgtag aaatgcggca tggggtcgat ctcattgtcc
    17941 gcctgcacgg ccacgatcag gcggttcaga tacgggcgaa tctcggccat gacggcccgg
    18001 atccaatggc tcgccttgcg gcggaactcc gcgtgatgct tgccaaaggg aatggcatgg
    18061 tcggggattc cgaggttgcg ccactcggag tagcaaaagg gcccgggtcg cgcgagcagc
    18121 ttgaagcccc gcttttcgac ctcggccagg aactcgtgca gcagggcgaa gtcgtattcg
    18181 ccctcccggt gttcgtgcat ctcccagatg atgtacgtcg ccagcacctc gacaccgagt
    18241 tccccagccg cgtcgaggag cgtcccccaa tgccgtcgct ggacacggcc atagtgcagc
    18301 tcaccgccaa tcattgcctg ctgatcaaag acgttcgaca tcgcctgttc ctgatgagct
    18361 ttccgtctcc aggggctcct tttccccgga atgcggccga ctactctatg acgggcttca
    18421 gccgcttgcc agccgcccgg gcaaagaagc cgagtcgtca ggacgaccgg ctggcgacaa
    18481 gtacaggccc gatcacaaag gtaaacggtt aacgaacgat tccatggcat cgtgacccga
    18541 cgtgtgcggt gggtaggtgg cctgcgctgc ggtgtggcaa ggtgattggg ctcggcccta
    18601 gggcgggcgt ggcggttgat cgcttccgtc ggcaccttcg ctggtatcga ccatagctcg
    18661 ggcggcttcg gtcgcgctgg ccgatgctga ggagccgacg gcgacggcca tactgcgaag
    18721 cacccgcagg tgccattggt ttttggcgcg ggtttcgtta tccttacaga ggtccttggg
    18781 gttcttcagg cttttgccag tccggcttgc ccgaggtatt ggtcaagatg atgccagccg
    18841 caatgctggg agctacttgt tgatcgcggc atccggggtc gcgggtgcgt cctgggttga
    18901 tggactgggt ggcggcttgt agtctgggta cggcagaagc aggatctctg caggataagg
    18961 tctgagacgg ccggttgggg cgcgtccggg caagacaatg ggcccccctt gagggcccag
    19021 agcggtcgta agttattgtg tttgcgtaag ttgttcccct agctcaattg gatagagcgt
    19081 cagcctccgg agctgaaggt tgtgggttcg agccccgcgg ggaacggttg atacaatccc
    19141 ttagccgaac gctgtttagc gtgcctaact gcgtcgggca atgcggcccg agagtcctta
    19201 acaaaaccgt agactacagt tttagcacgg acagaaggcg gtacaccccc gaccgaagta
    19261 tcgcgtcccc tcgtattgtc tgcagaaagc gagtggtcag ccggtcctca ccattggtag
    19321 ctaagacatc tatctcggtg ccggttccgt aagttttgtc cacctccttt ccggagattc
    19381 agactcattt tcgacaccgc gattcgcgag tcttttttct ggcaagaagt tgcgcgcccc
    19441 gcgatcgggc ttgggattac atggcccact gtccctccag ttcgttcagg tctgcccgca
    19501 gagtgacctc agacggcggc tggacgccgg ctgcggcgaa gatctggcca gcgctgccct
    19561 gcagatccgt ccgcaggcga tagcgccggc ctcccatctt caccctcacc gcctgaatgg
    19621 ccgacagatc ccgcatcagg gccggccacg acccatggat actccgctca tccaggtgac
    19681 gttgcaggta cgccgccggt ggccttgtcg gtcggcagac ggcacggcga ttgtcgaaaa
    19741 cgagtcagaa gcggccgcag caggtcatcg gctggtcttg cctgtgggcg gccggaatct
    19801 acttcggcag caaaggccgc agacgctcgg caagcaatcg gtacccctct gcgttgtagt
    19861 gaagcctatc ggcgacgaac aattcctcac ggagctggcc gtcggatctc aagaccagat
    19921 cggacgtttc caggtacttg aggcacggcc gttgacgggt gtagttctcg atgagggtat
    19981 tgagcgtctt gttggcctcc cattcgggcc aacgggcggg cgtggggttg tttgagatgt
    20041 acacgatttg tgtgttcggc aatctcgcat gaaccttggc gacaaaagct ttgtagtcgg
    20101 cgaagacctg ctccggggac ttgcctgcat gaatgtcatt gccgccggcg cggaggacga
    20161 tcatccgcgg ttcgtacggg aagatgatgc gctcggcgaa gtggacggca tcgatgattt
    20221 gcgacccgcc gaagccgcga ttcagcacct ggtgatcggg aaaatcctct gcgagcgttt
    20281 tccaccgtcg gatcgtggag gacccaatga agagcacgcc gcctttgggc ggagggtgaa
    20341 ttcgatccgt ggcttcgtac gccgcgatgt ccctttccca tctggcgaag tcatgcccgg
    20401 tttcggcggt cgtgttcgtc gccggagcag tccggcttgg agtcgccggg cgcgactcgg
    20461 agcatgcagg caaaaccgtg ctcagcaaga cacaaaacaa gaatgtcgtc gcggatgtgc
    20521 tgatgagtct catacaaatc gtcccttgcg tgaacacggg cggcgatcgc cgccgcaggc
    20581 ggattctaca cggaagagcg ctttgaccgg aaccacgttc tgccggtcga cggtccgcga
    20641 tggctcctgc gccttactcg ccgtcggagt ggtcacgcgg cttgcctatg agttcgcgga
    20701 cgacgtacaa aggccgctgc ttcacctcct cgaagatgcg accaatgtat tcggcgatga
    20761 ttcccagaca gagaagctgc acgccgccca ggatcagcac ggtcatgatc aaagcggtcc
    20821 agcccggctg gatcttgctc gcatcgccga agaggtaatg ataaagaatg aaggccgcat
    20881 aggaacagcc gcaaagaatg gttaccaaac cggccaggga ggcaacccgt aacggcaggt
    20941 tcgaaaaaga gagcacggca ttgagggcca tttgagcggt ctggcgaacg gtgaaagccc
    21001 gttcgccggc gaaccggtcg cgatcggtgt agccgacacc aatctggcgg aagccaagcc
    21061 aacttatcat gccgcggatg aatcgggcgt gctcgggcat gcgccgaaag gcattgacca
    21121 tagggcgtgc cataaggcgg aagtcggcgg cctcggccgg aacatggatc ttcatcaggc
    21181 tgttgatgaa ccggtacccg aaacgaggtc tgacgccttc gcggtacgca taaacgaggt
    21241 cgtagcccgc gcgccacttt cgcacgaact ccaggatcag ctccggggga tgctgaagat
    21301 cggcgtccat gaccacgacg gcgtcaccga tagcatgatc gatgccggcc gagagcgccg
    21361 cctggtggcc gaagttgcga gagagggcaa ccaccctgac acgcggatcc tcgcctgccc
    21421 aggctttgag cttgtcgagc gtcgcgtcac ggctgccgtc atccacgaag acaaactcga
    21481 cgtcgtcgat cgcgtaccgg gaatcggccg tcagttcccg ggcaactgcc tcgagttgtc
    21541 gtcgcaattc ggcgatgtta tccgcctcgt tgtagcaggg agcgatgata gacaggcgca
    21601 tcatgcttct ccgaggacgg cgtgaagttt ctccagttcg ccggccttca tgtgagggac
    21661 gtcggcctgc gtgggaaaac gacctgcgtg aatatcatcg acataggacc tgaagactct
    21721 catcaagatt gcggcgaggt tttcgtattg cttcgatcca cggggcggat ggcccatgcc
    21781 gagcccgagc agatcgtgaa gtacgacgac ggttccgtcg cagccgggcc ccgagacgca
    21841 gccgatgacc ggcaggctgg tgtgggaggc gatctcagcg gcgacttctc cggcgacggc
    21901 ttcgagcagc agcatgcagg cgccggcctc ttcgagtcgc ttggcgtcgc tgatgagttg
    21961 agcggcgctt gcggcgtcgc ggccttgagc ccgataaccc acttgcgggt cgatatgctg
    22021 cggcagcaga ccaagatgcg cgactaccgg gacgccgcga cgtgtggcag cttctaccag
    22081 ggccgcgtcg cggccggtca cctcgatctt cacggcgtcg gtaccgggtt cgacgtggaa
    22141 gcgtcgagcc cactcaacgg tggcatcggg gttctcttca acgcggcagg cgaagggcag
    22201 atcggccatg acaaatgagt tgggggcccc tcgtcggacg gcagcggtca gggcgaggag
    22261 gaagtcgggg gagatgtccc gcgtcgaggg taagccgagg acgacttccg cggcagtgtc
    22321 tccgaccagg agcacctcga cgcccgcctg ctccatcagg ccggcaacgg cggcatcata
    22381 gcaggtcaac acgctgaatt tcttgccggt ctgcttgaac acccgcaggt cttcgagggt
    22441 cggtttggaa cgcgcttgca tgatgaagat aggatacaat gggccaaaag gctctacaag
    22501 cagggggctt gcatcgcgcc gcctggcggc tgataataca acgtccgtcg ccagtcggcg
    22561 gatcgcccgg cgcggcgttg ttgccgcagg ccctgcctgt ggaggcggcg cggggatgga
    22621 attgcttgac aaccctatat ggtcggtcac gtgccggcag atcggaacag acgaatgggc
    22681 gcgaccctcg atgccttgct gcgtcttcaa cgtatcgaaa ctcaactccg cctggtccgc
    22741 gggcaaatcg aaacaaagcg acgttccgtg caagcacaac agcgccgact ggccacactg
    22801 gaaagacaaa tcgccgacac gcacgaactg atccggcaag cccaagccga ttcggaccgg
    22861 ctcgaactgg atcgcaaggc acaggaagaa cacatcaaac gacttcgcga acgactgaac
    22921 cagaccaaga ccaacaagga atacgcagcg gttctgacgc aactgaacac cgacaaggct
    22981 gatgctctca agatcgagga caaggttctg gcggcgatgg gcaaagttga cgatctcagg
    23041 aagcgggaag ccgagcaccg ggcgacgctg gaaaaggaaa aggcccgcat gggtgagacc
    23101 ctgaagtcgg tcaccgagtt ggaagctcaa ttgtccgccc agatcagaga gttggaggcc
    23161 caacgcgatg cggccgccga agaggtgcct ccggaagccc tcaagacgtt cgaaagagcc
    23221 cgtgacaggc acgacggtga agcaatggcg ctggtcgagc agacgcatcc gcggcgcgcc
    23281 gaatatatat gctcgggctg caacatgtcg gtcacgttgg agacgatcaa cgcgctgcag
    23341 tcacgtgacg acgtgcaggt ttgccacata tgttcgcgga ttctgtacct tgattcgtcc
    23401 gcaggggtgg cggcccggta ggcaaggcgg ccttgtgtca tggcctggac ggcgcacatc
    23461 gacggcggtt cacggggcaa ccccggcccg gcaggtgcgg gcgtttccat cgacgacgag
    23521 aaaggtcggg aggttttcgg tgccggattc ttcctcgggc acacgaccaa caaccaggcc
    23581 gagtatgccg gtctcttgcg ggccctgaag gtgttgcggg ccgccggcgc cgacgacatc
    23641 cgggtactga ccgacagcga gttgctggca catcagatta acggcatcta tcgggtaaaa
    23701 tcagccgaac tgcacccgct ttacctggag gcgcgaaagc tgctgaaagg cttcgaccgg
    23761 tgggacgtcc ggtacgttcc gcgggcacaa aaccgtttcg cggacagact ggccaaccag
    23821 gccatagacg ctcgaaagac ggtcttggtg cgcgaccggc tggatctggc caggcacgcc
    23881 gagttggact tgcccgccgt caaggccgag ggcttgttcg acgcaaagcc gttgagcgat
    23941 catcaaggag actccaggaa agggcaggtg gccacgagcc gggcaaggtc ggccgtctca
    24001 ggcatcgagg tcgtggtggc tcgcgcgccg cggaagggga cctgccgggc ggcgatgaag
    24061 cagggtgatc gattcatctt cacggaggtc acgccgagcg gtatgtgcgt ggaggcttgc
    24121 gcggcggtga tggaagcggt gctggcggtt cgcgatgccg ccgctgccgg tgtaacctgt
    24181 gacgaggcca tgagttgccg ctgtccgcat cctgactgcg gggcagtctt cgacatccgg
    24241 caggttcagc aagtccgata gccgccgatc cgacggtcgg gggcgagtgc tggaatcgcc
    24301 caaaacctgc ctgaatgacg acgctgattt cgtcctgcct gtctgctttt catgtctggt
    24361 ggtgcgaccg ataagcttct tgatttgccg caatgagcgg gaaagtcgcg gagatacgct
    24421 cgcaatttcg ggccggcccc gcacgtaaga gccgtggcgt caccggcttg tgccgctaag
    24481 gggcatatgc gaacgaaccg atatgcccga cggggaaaga ggtgtgcggg cacagggcag
    24541 agaggcgcgc cggtggcccg aggtctggca tgatcaggtg gtgtatcgta ctggggacgg
    24601 gagccgcgct gttgtgcggc tgcgtggagc caaccggcag gtcggttgtc atttcgccat
    24661 tacctccgcc ctactacatc ccctacgtgc atgacgagcg tccggtgacg ctgaccacga
    24721 tgccggccga ggaggagccg gatccgacca aggcgtggta tccggacggg ccgcttttcg
    24781 aggcctgggg ctttatcgtt attcaccata ccgcaacacc ttatggttcg atgcgggaga
    24841 tcgatcgcgc ccatcgcaac aacggttggg atggttgcgg ttatcatttc gtcatcggca
    24901 acggcacgcg gacgccggcc ggtttcatcg aaccgacacc acgatggaag agacaggaag
    24961 agggtgctca tactcggctg agtccgaaga tggcgcgcaa gtcaggggtg caaccgacat
    25021 attacaatga acacgggatc ggggtcgttc tggtgggaaa tttcgatgaa gtccggccga
    25081 gtgcggcaca aatggcctcg gccgccgagt tggtgaagtt tctaatgaag ctttgccgga
    25141 tacccgagga tcgggtcatc ggccacagag atgttgacgc gacggattgt ccgggcagat
    25201 ggttttccat ggatgacttg aaacaacggg tgcgggcgtt aaagtggccg acaacgatgc
    25261 cggtggagac ggcctcagtt cgtccgagcg gccgatctca atagcgcatc gagcaagggg
    25321 gccttcaatg aggcgctacg cgaacgggcg aggcatcaca aggtggttga cgtcactggc
    25381 ggtcggggcc agcctgctgg gagctttggc gggctgcaga gaatcggtta cgcttgcttt
    25441 ctatgaggga ctggagaacc ttgcggtcgc cgtggtgcag gccttctttg atgctatcac
    25501 tccaacctca tcatccactg ccggcgctct ggacctcatg cactcagctc taccctggtt
    25561 ggcctgagga gtaaaggaag ctgacggtct tcccaacatc cgtttccagg cagtatggga
    25621 ctccgccgga agtgggcgct tgggtgaagc ttaagttggg gcgccctgtc tgccgcttct
    25681 ccgtcgagga gatcatcctg actctttgcg acgcataccc ggtactggag gtcccagcta
    25741 gacagtactg tggcagagag ccataggtcc cgttgactcg aatcccaagc cggaaagtta
    25801 ataggctcga tcagcatcca tgcgcccacc ctgcttgctg gcaataagca cttgttagga
    25861 aatcagttac ggtcgatcaa acaaacctca gagaaataat tgctattttt tgttgacgcg
    25921 caatagatcg tggtgtacat tgtgtttggt tttagttagg tatacgaggt ctttgatcgg
    25981 tgaaggtcgt ttctggtgac gcggaggttg tgatgccggg ggtggcgagg ccaaaggaat
    26041 cccgggtgga ggggaagggc aacgagtcgg gtgccggcag gtcggtgggg cgggtggcgt
    26101 tgatggtgac agttcccggg gtggccgccc cgccgacccc ccggctcgtt gaggtgtgcc
    26161 tgaggttcgg cctttctttg acgcagggcg agcgggttat cgtctcgggc ctggttctgg
    26221 acctgcggcc aggcagtgtg acgatgattg tcgggccttc tggggcggga aagacacttc
    26281 tgattcgcga gatcgcacgc cgtgcttcaa tgtgccaagt ggtcggagac atggatgtgc
    26341 cggcggacgt gccggtgctg gatgcggtcg ctccgacgcg accgatcgac gaggcgatcg
    26401 ggttgttgac ggcctgcggc ttgggagagc cttcgctgtg gatgcgtttg ttcgatcaat
    26461 tgtcggaggg agagcgtttt cgcgctcggc tggcgcgggc gatttcgctg tggcggcgaa
    26521 acggcaggca cggcgccatc ttgtgcgacg aattcggctc gcttctgcac gaacggcttg
    26581 ccaaggcggt tgcgttcaat cttcgcaaac tcgcaacgcg cgaccgactg gccgtggtag
    26641 tcgccacttc gcgagaggac atcgagcgag atctccgtcc ggattgtgtc atccgccttt
    26701 cagacaagcc ggaccttgta cacggctccc gagagcgaca tcccgacggc gcaggagcaa
    26761 ggctgtcgtt cgccgaggat ttgcaggtcg tgccggggac gctgcgtgac tatgaggcct
    26821 ttgccgcgat gcactatcgg caacggcaac aggttgggtt tgtctcggcg gttttcgtaa
    26881 tgcgtgatcg gaggaccggg caggcgttag gtgtggtggt atacggacat cccagccttg
    26941 aattggcttt gcgtaacaag gtgacggggg ggcggtacgt gcgacaggca aagctgctca
    27001 atcaggaagt ccgggtgctg aaacgcctga tcattcaccc ggatgtgcgg ggctgcgggc
    27061 tgggacacga actcgtgagg cagacattgc ccatggcagg tgcacggttt gtcgaatgct
    27121 tggcggcaat gggggccgtc aatccggtat tcgagaaagc cgggatgcga ccgatcgggg
    27181 tgtgctgcgg ttcggcgggt tcagaggcgg cgctggcgtg gctcagggcg gcgggggccg
    27241 acccgctgga ggctgcattc acatcgcatg ttcgttgccg gccggcggtg cggcgagtcg
    27301 tcgcgcgcga ggtggcgaac tggtaccgag ccacgacggg cggcggagag caacgggttc
    27361 ggaaacagac gcctaccgtt ctggcccaga cctatcggca gcttgcgggc tcgcaaccgg
    27421 tgtatttcat ctgggcggcg gcaaacgaag atcgggagct catcgaggcg aatcttggag
    27481 acgccgagga agcggcccag gcgtcgtgag gagagcgacg atgtcggacc gaggggataa
    27541 acccctcggc tcgtcaagag acgcggctgc aagcgcttgt acggaagacg agaggtgggg
    27601 aatgaaagtg atggatgtgt tgcggaaggt ggtacggccg ctgttgtcgc gcaaagtccg
    27661 cgtggcgttg gcgacggtgt tggcggcgta tgcggccgag tatggcctgg gagtcagcga
    27721 ggagatggtg ctgaccattc tgggcgtggg ggcggcgttg atccttggaa tcgcccatga
    27781 ggatcagggc ctcaagcagg gaacgggcgg atcggtgagg agctgagaat gggggttggt
    27841 gatgcgaacc ggacgggaag agatgttgcc ggcaacggcg tgcatgacgg cggtgattct
    27901 agggttagcg gggtgcggcg gcgatgcgcg gctggaactg gcggcggccg acgcgctgct
    27961 ggcggccgcg gatcggatgg agaccgtcgt ggccgagtat cacgcggaaa tgaacgagta
    28021 cgacggaacg cgcgagtcgg cgctgataga ggcgtttgtg gagcgggtaa aaagcagcgc
    28081 cggcgacgac caggcgacgg cgggccatgc ggcacagttt cgcctggcct tggagcggat
    28141 ccgacaggac cgagcggtgc agtggcggcg gcacacggcg gcactggaga acgtggctgc
    28201 cgtccgcgag gtggccgaag gactgcggcg gatggggatc gagagcctga cgctgagcga
    28261 cgagatgcgg cggtacctga gcgggtggat cgaagcgagg aaacgagcgg cgaggcccac
    28321 tgctgtgatt cagacgagct cggccgggaa ggagtgcgac catgacggcg agtgatgcga
    28381 ccagagtgct ggacgacttg atatcgacgc tggaacaagt aacaccgcgc gccggcgaag
    28441 gcgatgcgat cgacgcggtg ctggcgggga aacctcgcgg cacgcaggtc gtctcggttc
    28501 ggcaggcgcc ggagatggag gtgttccggc gggaacttgt ggatgggctc attcgggcgg
    28561 atacggtgaa ccaggtcctg cggctgctga acgagtgggt catggcgggg ttggcgcgat
    28621 aggtgaagcc atgggatcgg agcatgttgg ggagccgctg gtcccagatt cccatccgct
    28681 ggtcccagat tcccatccgc tggtcccaga ttcccatctg ggacctcccg ccgacgattc
    28741 ctatcgtcat gcgtgtatga atgcggaaag gattccgcga agacgtaagg cccggaatgg
    28801 attccgggcc tagcgaggaa tggattccgg gcctagcgag gaatggattc cgggcctagc
    28861 gagaaatgga ttccgggcct agcgagaaat ggattccggg cctggcaaaa acaggattcc
    28921 gggcccagca agggatgggg cacgggcagg gcctggcgac agagagcaga gaggcacggc
    28981 gatgggatgg gagcaggttg gggtattggt aggagtgttg tcgccgctgg tgggcgtccc
    29041 gttggggatg atcacgctgt acctgcgggc cattcgcgaa caacaggcga caacaacttc
    29101 ggagatctcg cggagaattc acattatcga ggcatcactg gacaatctgg tgaagcggat
    29161 ggcggaatgc gaccgcgagt tcacaacgaa ggaagaatgg ctgcgagagt cgatgctggc
    29221 ccgacagcgg ctggagcggc tgaccgaact ggtcacgcgc atccaggcgg agcttgacgg
    29281 cgaacgaggt ctggctacgg agatcggccg ggcgacggag gcgatggtgg agttggtgcg
    29341 gaggctgacg ggagcggggg agagggcagt agagcgggtc aacactacgg cctccattgg
    29401 gaagtgacca acgacccgat cccaatgacc gatcaacgac ccgctggtcc cagattctca
    29461 tctgggacct tcctgctgac gattccgatg gtcattggtt catgaaatgc ggaaatgcgg
    29521 aacggattcc gcagagggtg cggcccggaa aggattccgg gccgagcaag aatgccaatg
    29581 acccatcagt gaccggcctt cgtccgggct atggcgtggc gagcccgaat gaagaagtcc
    29641 caacgacgac tgcagatgac gggttgacgg ccgcggagtt gagggttgga gggggatgat
    29701 cagagatttg aaatctcaga tttgagatgc cagattgggg atctgcgatc tgacggactg
    29761 cgcattgggg cgcgcgaatt gcacgatgct gaagggagga gggtgcgatg ggcaggaacg
    29821 ggggcgtgcg gcagatcaag cgggtgcgga acgaggtgct ggtggcgttg aagatgttgt
    29881 atccggcagc gctgcagggt gaccaggttc tgcggacgct gctggcggta tttccgcaac
    29941 ttgagtggga tcagctcaag aaagacctcg cgtatctatg tgagaagggc tacgtgcagc
    30001 gtatcgtcgc cgacacggaa gaggatgccc ggatgacttc gtggcgcaag cgatggtttc
    30061 gactgacctc aagcggggtg gaggttgccg atcactgcgt ggaggacaag gcgctggaag
    30121 tgtaacgttt gttcagtggg cggtgttatt gtttcgcttt tccggtggcg ggaggatatc
    30181 ggcctggtcg ttgcccaggg ccttggggtc ctcgttctcg gtctcgcgca ggcctttctt
    30241 gaactcaacg atgctgcggc cgagcgattt ggccaggtcg gggatccgtt ttccgaagat
    30301 taacaagccc accaggccga tgatcacaat ctccatcggt cccaacgtcc acgcgacggg
    30361 ttggaagaca aggttcatca gagactcctc aaccggggct ggaacaggcg gcccagccat
    30421 tctgcgggtc tggaggaccg gtcccgaaaa cggcattgta gatcattccc gtggcggggg
    30481 tcaaacctgc cggttgcttc ccgatcagga gcccggagcg ggctgggttt gcgcgacatg
    30541 gccgaaatcg gcgggggtcg ccgtcgggtt gggcgcattt ccgggttttg cgtcgtggtc
    30601 cttgccgacg atcccgtcgc taaccatggg catgccgggc ggatgcacga cgacgctgac
    30661 cggcaaggcg caggtattga ggatgaagcg acccaagccg tatggcatgt ggagatagtc
    30721 ctgccaggtc caggcgaagg cgtcgtccat gtcgccctta tccacgaacg gatcctgcca
    30781 ccacaagggg tagtggggca ccgcgccgtc ggccaatcgc aattcggatt cgggcaaggt
    30841 ccttttacga atcaccggtt cggggcctgc ggcaaggaag ccgtctcggc tgggggtggt
    30901 gcgagcctcc ttgccgatgg cgtcgtcggc ccacggattg acggcgggcg gctggttgca
    30961 ggccacggac gcggcggcga cgattagcgc ggttgcgatc aggcgtgcgg aagtcttcat
    31021 agtggctgct ccacttggta atcgatggcg tagttcggag cctcacgggt gatggtgatg
    31081 ttgtgggggt gattctcgat cacgctggcg ggcgagatgc gaatgaagca ggccttggag
    31141 cgaagctcct cgaccgtcgg cgttccgcag tagcccatgc cggcccgcac gccgcccacc
    31201 agttggtaaa caaattcggc gagctggcct cggtagggta cacgaccctc gacaccctca
    31261 gggacaagct tatcggccct ggtctcgccg ccttgaccat aacgatcggc gctgccgctg
    31321 accattgcgc caagcgaacc cattccgcga tattccttga agcgacgtcc cttccagatc
    31381 acgatctggc cgggggactc atcgagaccg gcgaacagcg agccgatcat gacggaactg
    31441 gcgccggcgg cgatggcctt ggtgatgtcg cccgaatagc ggataccgcc gtcggcaatg
    31501 acgggtacgc ccgcgggctc ggccaccgcg accacgttca tgatggcgga aacctgcggc
    31561 acgccgacac cgctgaccac acgggtggtg cagatggatc cgggaccgat accgactttg
    31621 accgcatcgg cgccggcgtc aatcaggtcg cgggcagcct cgccggtggc aatgttgccg
    31681 gcgatgatgt cgatgttgtg tttcttacgg atgttgcgaa cggtctcgat gacgttatcg
    31741 ctgtgcccgt gggcggtgtc gacgacgatg acatcaacgt cgttggcaat cagagcctcg
    31801 actcgctcat agtcgtgtac gccgacggca gccccgacgc gaagccggcc tcgagaatcc
    31861 ttgcaggtcg tcgggtagag ccggctgcga ttgatatcac ggatggtgat caggccggcc
    31921 agtctcccgt cgctgtgaac cagcagcagc ttctcgacct tgtgccggtt cagaatccgc
    31981 tccgcgtctt cgagggtggt atcgggagcg gccgtgacca gattggtctt ggtcatgacc
    32041 tgctcaatga ccatgtcgtt ggaggcgggc gggagaaact tgaggtctcg gcgtgtcaag
    32101 atgccccgga ccctgctgtc gtcgtccgta attgggacac cggacacgtt atgcaggttc
    32161 atgatctcgc gggcccggcc gacggtgtca ttggggcgaa gagtgatggg atcaatgatc
    32221 acgcccgatt ccgaccgctt gaccttatag acctcgcgtg cctgggcctc gatggagagg
    32281 ttcttgtgaa tgatacctat gccgccctct tgggccaggg cgatagccaa ggccgattcg
    32341 gtgaccgtgt ccatgggggc cgagatcagc gggatgttga tggggatgtt ccgggtcagg
    32401 cggctgcgca cgtccaccac gcggggaatg acggaagacc gtgccgggat cagcaacacg
    32461 tcgtcgaagg tgatcccttc gccggtgatc ttggagccgg gatatccgga ccggttcatt
    32521 cgctcggcca ctttgcgggt atcgttgacc atgatgttat atagcggcag gacggctggg
    32581 gcgtcaaggg ttcagggttc ggggttcagg ataaggcgga tggatgaagg atcaattgaa
    32641 ggggtgggag actcggggtc cgggagaggg ctgaggggca aaggatgaat tgaagaacgg
    32701 ttgacaactc acaggtcgcc gctgcggaat gggctggaag gcttgccagg gactgatcgg
    32761 ggtctttaag atcggtccag cagagaacag cacgaatggg aaggcgagtc gttcgggtct
    32821 cttgcctgcc cgtgttccgg tccagcggga aaagaccttg gcgggcaagc ctccggtggt
    32881 acccggggcg ggtcggaagg acgagatcat cgctccccca cctgcaagaa gcaggtgggg
    32941 cacccgccga gtgatacggc aggtctcggt cgccttggcg acctcgacac cgccctaccg
    33001 gtcgggttcg ttggttttca cacaggctgt tcgaggcggg gtttgacagg agtggcaggg
    33061 atgtcgccgg ggaaattcac acatctgcac gttcacacgc atttcagcct gctggacggg
    33121 gcggtgcgaa tcaaggatct ggttgagaag accaagctgc agggctcgtc ggcggtggcg
    33181 atgaccgacc acgggaacct gttcggggtg gtccctttct accaggcggc ggtcaaggca
    33241 ggggtaaagc cgattctggg gtgcgaggtg tacatggccc cgggggaccg gcgggagcgc
    33301 gaatcgcggg gcattcaaga agccagctat cacctccttt tgttggcgcg caaccgaacg
    33361 ggttacggcc atctgctcaa gctcgcctcg atcgcctacc gcgaggggtt ctactatcgt
    33421 ccgcggatcg acaaggaggt gctgcaggcc caccgtgagg gcctcatcgg catgagtgcg
    33481 tgcctgggcg gcgaggtacc gcaggccctg ctgcgaggcg acgtgcaggc ggccgaggag
    33541 atcacccgca cctatctcga cattctcggc gaggacgggt tcttcctgga gctccaggac
    33601 caggggatgg acgagcagaa ggtggtcaat ccggcgatga tcgacctcgc ccggcgtctc
    33661 ggcgtgggtc tggtggcgac caacgacgtg cattatttgg atgtcgagga tgccgaagcc
    33721 cacgaggtgc tctgctgcat cagcacgggc aagaggctga cggacgatga ccggatgcgg
    33781 ttgccttcga acctgttcta tctgcggtcg ccggcggaga tgtgggaaac gttcaaggac
    33841 gtccccgagg ccctggagaa cacccagcgc atcgccgaca tgtgcaacgt tgagctggag
    33901 ttcggcaaga cgcatgcccc ggtgtaccgc gtgccgggcg gcaagagcga ccgtgaatac
    33961 ctgcgagagc tcgtctacga gggggccaga gccaaatacg gcgaaatcac cgacgagatc
    34021 cgcgagcgaa tcgactacga gctggaggtc atctcatcga agggcttctg cagctatttc
    34081 ctgatcgtgt gggacttcgt gaagtacgcc cggtcgcggg ggattcccag cggagccagg
    34141 ggcagcggat gctcgacggt agtaggttac tgcctgggcc tttcggcgcc ggacccgctg
    34201 cgatacggct tgtatttcga gcggttcatg gaccctgacc gcgacgagat gccggatatc
    34261 gacattgata tctgccagga gggccgggaa gaggtcatcg actacgtgcg gaagaagtac
    34321 gggcaggtgg cccagatcat caccttcggg accctgcagc cgcgggcggc ggtcaaggat
    34381 gtcgggcgcg tgttgggtct cggtttcgag gaaaccaacg ccatcaccaa gctgattccc
    34441 gaagagctga agatgaccat cgacaaggca atggagaagg agcccgatct tcgcaggctt
    34501 tactcggata acgagcagat ccgcaaggtc atcgacgtcg cccggcgact ggaggggctg
    34561 tcgcgacaca ccggggtgca cgcggccggc gtggtgatct gcgacgagcc gctggagaac
    34621 ttcatcccgc tgtaccagcc gcccggcacc gatcaggtga tcacgcaatt cgacgggcca
    34681 agtgttgagg cgtgcgggct gctgaagatg gattttctgg gcctcaagac gctcaccgtt
    34741 ctcgagcggg ccaggcaact ggtaaagaaa cagcagggga tcgacctgga cctcgacagg
    34801 atcgaactcg acgaccagaa cgtctatcag ctcttcgttc gcggcgagac caagggcatc
    34861 ttccagttcg aatcgggcgg aatgcgggac gtggtgatga agatgaagcc gaaccgcatc
    34921 gaggacctga ttgcggccaa cgccctgtat cgtcccggcc caatggtgca catcgacgcg
    34981 tacgtggccc gcaagcacgg cgagcaatgg agcactccgc acccgatcat gaccgaggtg
    35041 ctcagggaga cctacggtat tctggtgtat caggagcagg tttcgcggct ggtgaaccgg
    35101 ctgggaggaa tcgagctgaa acaggcattc cggctggcca aggcgatcag caagaagaag
    35161 acggccatga tcgagtcgat gcgcgagccg ttcgtgaggg gctgcgccga gaagggagtc
    35221 ccgcgccgga cggccgagga gatctttgag gacatcctgc gtttcggcgg atatgcgttc
    35281 aataagtcgc actcgaccgg ctatgccctg gtggcctaca agacggcgta catgaaggtg
    35341 tactacccca ccgagtacat ggcggcgctg ctgacgctcg aaatggattc gaccgacaag
    35401 gttgtcgagt acatcgagga atgcaagcgg atggggatcc gggtgtcggc gccggacgtc
    35461 aacctgtcgg ataatgattt cacggtgatc cgatccgagc ggggcaaggg cgagatccgt
    35521 tttggattgc aggccatcaa gggggtgggc gagaaggcgg tgagcgcgat tcgggcggca
    35581 cggagcgaag gtgggccgtt caagtcgatt ttcgactttt g
//
LOCUS       DAOTPW010000019        35502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1290_length_35502_cov_5.839338, whole genome shotgun sequence.
ACCESSION   DAOTPW010000019 DAOTPW010000000
VERSION     DAOTPW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 35502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35502
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_35502_cov_5.839338"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="P5316_02980"
     CDS             complement(<1..518)
                     /locus_tag="P5316_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83949.1"
                     /translation="MGGESSGKGRRLRRVDFRRNRQTPARRKRWEIPTDDDQGTEVFS
                     REAVKAKGDLSRKRTVVEIDQDQSRGEGSWLKGIAVAVRGQFVDVDVDGRIWPCTVRR
                     VLRTLLIRNRSPVVVGDEVAISIVADEAGVVNEGVIEHVYPRRTALSRCDGKKTHMIA
                     ANVDQVLVITSIR"
     gene            777..1721
                     /gene="pyrF"
                     /locus_tag="P5316_02985"
     CDS             777..1721
                     /gene="pyrF"
                     /locus_tag="P5316_02985"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRR83950.1"
                     /translation="MAENFADRLLKAIKAKGSPTCIGIDPVYSKLPAEISENKELNDA
                     TDSEVALDAILEYCRRLVRIVSPLVAAVKINIAFFERYYADGVDAYFDLVQEADRAGL
                     IVIGDCKRGDIGSTAEMYAAAMLAEPDFANLEDLTGPDAITVSPYFGLDGLQPFINVA
                     REEQKGVFVLVRTSNPSAAELQTARLESGMTVAEFVARQVESWASDPTLVGECGYSAI
                     GAVVGATARDEIATFRAMMPHCIFLVPGFGAQGGTASDIGACFKPDGTGALVTASRSV
                     IYAYDDMKYLERFTSEWEKCIEQACKDFVAEVRNVVPR"
     gene            2017..2988
                     /locus_tag="P5316_02990"
     CDS             2017..2988
                     /locus_tag="P5316_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR83951.1"
                     /translation="MAHYHQASSCLPTRLPACAMLLVVLVLGTLCSADAHRFVIVSDL
                     HPTHAIIDQLDRLTREVLEIRPAFVVQLGDVGNDSFPGESVWQMKVIDAAFRRWREAG
                     IEIHVAVGNHDVPADKPGVAVLKRNWFASQLPPYPMNAQLDADKHPEIWKRYCLEGKH
                     YYAFTWRGVHFVIMDSENITDHQLAWLEAVLCRHEGNPSRYPTLVFAHDPHVMTGDRG
                     HISRPLYRILEKCPDGHCVQAVFGGDFHYAQYWPPEENLGAHVYAVPASVLNFAIHHP
                     QEPAYTEYIIATVTEDEIAFEPKPIGRGVNIFKDRVSYHPIARRRAD"
     gene            4151..4621
                     /locus_tag="P5316_02995"
     CDS             4151..4621
                     /locus_tag="P5316_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83952.1"
                     /translation="MVHAAVRGPAGGTQLGPRPGHLPPAATVSPDYDLAPALEHCRCG
                     IVNFYSREDWFDAGVLTKTFGTMDRKETSTAGRRGFLDAGGTLCVRDGLNQAAWTPQW
                     RKLGHWGGHLGYESRTWAPEVLAPLIPSASGPPTGQIEGATARSAHATIPDYPS"
     gene            complement(4625..6046)
                     /locus_tag="P5316_03000"
     CDS             complement(4625..6046)
                     /locus_tag="P5316_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83953.1"
                     /translation="MTKPNVHLESGISVFDRCHDLAVETLCRNIKPWKDGLLDEPAPS
                     IMAGQTYKSPWTRDCAFNVWNAAALLTPEAARNTLISTLIEDDGLVRIGGQYWDAIAW
                     TTGAWHYYCVTGDHEFLELAHAATVNSLRYFEETELDPEMGLFQGPASYGDGVAAYPE
                     PYNDAGGSSGILDWPAAHPEVGKIRMKALSTNCLYANAYWIADLMGRRLGLDAQVNES
                     LRTQGDAVGGAINRHLWLPEKGCYAYFTDPTGRCEQYMEGLGHAFAILFGIASPEQAR
                     AVLDNQYVAPYGIPCVWPVFPWFASPDGMTFGRHNGTVWPFIQGFWASAAAMHGRLDL
                     FDKELTALADMADRHREFKEIYHPITGEPYGGLQVDRGKVRLWAGEPHQTWSATGYLR
                     MIYYDLFGMRFQPDELCFQPTVPPRFETLQLTGVKYRDMTLDVTIRNSGTRIASFALD
                     GQPHEPVIPATLDGQHRIVMNMR"
     gene            complement(6252..7517)
                     /locus_tag="P5316_03005"
     CDS             complement(6252..7517)
                     /locus_tag="P5316_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83954.1"
                     /translation="MCLLDMTVGRCCGLVILLVVVASGIAAPTSEPSARTRPAETTLR
                     NRRILERVRKQTPGRTPARFNAIPDRLADILIEKHLYKDGEVWGLAKTVPDQGYPYDE
                     MGSCTFAGLVRQYNLYDKVGPLDDAARTKALKFWQSWQDPATGRFKDPRDPKRQVNEK
                     YVVGLIGQLGGEPLYKWTTTGTDKKIETRVFLERSREDPDWQNGGWGVGSHTGFQAVE
                     IFEAINSGQVELIPDLEKGVQQILSHQDPDDGLWGPPSAELMRRIGGTLKVIGRLYFK
                     MGMHVPHTRELADAMIEHSRNGDWYKHGANSCVPRNAAEVTAYCLEVSDYRYDELLEV
                     LESLAKDYESWVLPDGRTLIARGDPSNVGLQYTTLYGLGIIAAYLHWEDCQLPNPLAD
                     NPRGQGYRYQVGLLPDGSVKVIDTGAGKP"
     gene            complement(7555..8862)
                     /locus_tag="P5316_03010"
     CDS             complement(7555..8862)
                     /locus_tag="P5316_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRR83955.1"
                     /translation="MRLLIPFLALAICASTVVGAQTPNIIYVMADDMGYGDLGCYGQK
                     LIRTPNIDQLAAEGMRFTQFYSGCTVCAPARCCLMTGLHTGHAWVRDNKSSILERVPL
                     RPEDFTVAELLKKAGYSTGVVGKWGLGEPETTGVPNKQGFDYWFGYLNQAHAHSYYPD
                     YLWRNEEKYTLEGNVNGQGKQYSHDLFTQEALSFVERNKDKPFFLYLAYTIPHGKLEV
                     PSDAPYSDRDWPQPCKNYAAMITRQDADIGKLMKLLKDLKLDDRTLVFFCSDNGAPDG
                     GGTLRHFSSNGPLRGTKGQLYEGGIRVPMIARWPGKIKAGAVSDQVWAMWDFLPTAAE
                     VAGISPPKNIDGISMLPALLSESQKQHDFMYWEFNQANLVQAVRMGDWKAIKHNSGKI
                     ELYNLKSDVGETNNIAAEHPEILARIEDYLKTARTESEYWPTR"
     gene            complement(9029..9958)
                     /locus_tag="P5316_03015"
     CDS             complement(9029..9958)
                     /locus_tag="P5316_03015"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HRR83956.1"
                     /translation="MISRSGYDIRPLTHERIQELAKGLEPAERRVLLEKGTEAPFCGH
                     LLNNKEKGTYVCRLCGLPLYSSDSKFESGTGWPSFYRPFDPDHIRHQRDISHGMVRTE
                     TLCARCGSHLGHVFEDGPPPTGLRHCLNSVSLRFYPAGKELPPESRPLKLETAYFAGG
                     CFWGIEDRFQQVPGVADAVSGYQGGKAVNPTYKQVCSGRTGHAETVRVTFDPAQVTYR
                     QLLEWFFKFHDPTQLNRQGPDVGSQYRSAIFAADDEQLKQAKAFIEEQAAKDRFRGKR
                     IATTIEPAGPFYEAEEYHQDYHAKHGGSCPLFQ"
     gene            complement(10356..12053)
                     /locus_tag="P5316_03020"
     CDS             complement(10356..12053)
                     /locus_tag="P5316_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR83957.1"
                     /translation="MRTVLIATYTHPVALGMRYVSSSLKSAGHEVEMIFMASKRDTAK
                     PDFAPPVIESLIEHCRDADLVGMGLLTNSFCRACFLTEALRRAGMKVPIIWGGTHATV
                     APDECLEYADVVCIGEGEQVMLQLAERMDRGRDPTDLGGLAFRAGGPFGNRGEVRNPV
                     GPLIENLDDLPFPDYELKSHWVADDGRLVRARPGNLRGALDRLRVETTRGCPYRCTFC
                     NNTTWANIYRGRGSWVRRRSIENVLCELEEMCRRFPGISAVNIVDDLFFVRPAEEIAA
                     FADGYTRRVGRPLEVDAHPELVTEDKIAVLSRMPIALVSMGIQSGSCDTLYNIYHRRT
                     PVDRIAMAMDLLHRYRLPAEYHYIVNNPYESDANIIETMRFVADHHRKAAALRIFPLA
                     LFPGSPLYERARSDGIIGARHEEAYHRTYTGSVHVFKLDYLAIMLRLVLHLRNLGAPP
                     WLTHRFIGLVTNRFVRRCLDRRICMSMLMGAYYLCRKSYKILLYQPIVRPVLRLRRWW
                     VLRTPAMKIDLALGEPVSVPPAPVRNPKPPSGGFKPKSQGLENGIEPQQQTASRRVR"
     gene            12440..13867
                     /locus_tag="P5316_03025"
     CDS             12440..13867
                     /locus_tag="P5316_03025"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRR83958.1"
                     /translation="MPPDRIEVMKSIRKLMQQLEQALSNPREEFGRFTRFVLFQIELW
                     RFCVRRLRENNVMAMGSALSFRTIFAMIPVLALALLVMKSVGALEDSRASLHRFLEAL
                     GFTQIITVQETRPATGTAPGEVPPGEVINVADRIEEVVDNVSSKLTFQRIGPVGGALL
                     IWTALTLLTTMEESLNRIFGARSDRSTPKRVLLYWSTMTLGPIVLAAASYLGRQTIET
                     VRDAPGASWLIMTLGWGGPIVVGVIVLALVYALLPNTTVRFQAALGGAAVAVPLWLVA
                     KWGFSLYVKNLVAKGNIYGVLGLLPLFLMWLNLSWWIFLFGAQLAHTAANLSVMRMAE
                     RAGNLVLGPSDLLAAALAIARHYHAGQGPMTAGAMAGVLNLPVDAVQRLIDRLDSGGL
                     LIRVDREPEPGYLLCKPPTRLAVIDVMSIGDPRGSGSSPEGCDKQLTAVVQELRDRTR
                     ASLEGLTLEDLLRRHAEPGPCKATG"
     gene            14211..16124
                     /locus_tag="P5316_03030"
     CDS             14211..16124
                     /locus_tag="P5316_03030"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR83959.1"
                     /translation="MGERPNILEILKNESGPAADYALLTALREVGAATAQAIVDLLLL
                     RKQKAGLCGLIELFHELDEPICRTIVEHVDELFPALRLAAQSKDDQVRLNVLEIISRG
                     YAYRAAYLVDTAMHDRVARVREAAADTLYALADQLLKTSPIPDLHHDKLDPARARGLN
                     VDLEDYMEDRRQVIGAIEGSLASYNIHLQPKVIETAMWFVDELDQKFWSIVGVPGSRA
                     AHAAISVIVGVKSPRLVPFCITGLHYSTFRAPLSKVLGLCTDMAFIEEWIRQSWRLGE
                     PKIHRAMASIKELAVLKRKAVELMQLPPALMRHLPRWIMATGLPEQVKLDFLKNLYRR
                     GDEQMRRSTVKSMLPCAGERVISLLKAISRDNDERTAAVARFELAHRFPLEYPPSDLL
                     EPPKAAAAPSGQVWGRPGSDLTFEKYWDIYDTEPDPQRREIGLRVFDGDASAVRLLAR
                     QMNAAEPSDRLKALRIATLLGLGEKFVQQIYRLSHDPNPEVRSAAVSALGRLPGPVSQ
                     HLLRTALQDSDARVQANAVEAIEHKGGEQITEDLLPKLASPDNRVRANAVKALLKLGV
                     REAAITLLRMLDDPNRAQRISALWLIENMGLFILASRVMKLAQADQDNLVRSRARKLT
                     GQITGQLPVGAAQ"
     gene            16121..16591
                     /locus_tag="P5316_03035"
     CDS             16121..16591
                     /locus_tag="P5316_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83960.1"
                     /translation="MSMSAGRSVFDLLAKAGLPLAEGSWYESVQHRFLFGEPELIRGL
                     FTAALAMGLVLLVIGMSYRWQKRRQQPAPTQPMALFRRVLSKMGLTVIERWLLWRLAR
                     NSGLEHPTALLISSRLYDGAVDRYCAGSGLFFARAGKAASFAAIRRRIFGEKNG"
     gene            16643..16900
                     /gene="yidD"
                     /locus_tag="P5316_03040"
     CDS             16643..16900
                     /gene="yidD"
                     /locus_tag="P5316_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRR83961.1"
                     /translation="MRVVVTILSHAVAFAIESCVRFYQVALRPLLIGSCKFVPSCSDY
                     FIEAVHEWGPIRGSWLGIKRLLRCRPFTMGGIDPVPRRNRP"
     gene            complement(16944..17819)
                     /locus_tag="P5316_03045"
     CDS             complement(16944..17819)
                     /locus_tag="P5316_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83962.1"
                     /translation="MPRPAVDPATDRIACDLPCVGCEYNLRTLRADGRCPECGKEVAA
                     SIRESDPNAIHLYWITELKGGLDVLYLGISFLSVAVITILIPLMTGGSGFGLLTAVFM
                     PCLLLGLFMLAIGTFVVIGARPPSWVGQGLSRTRVVFFVGILGTVMLIICAIMLAPLW
                     WLSASVAMLAVACAGSIPISFSYYCCQLAFRYPTDRLRKTATVGVWATALCMSTLVIQ
                     LFMLILEEAWDISIVICPGSTLFVMATILFLGELPLYIAFFHKLREAITEELNRFSSA
                     PLADGGGLPTDSTGG"
     gene            complement(17970..20933)
                     /locus_tag="P5316_03050"
     CDS             complement(17970..20933)
                     /locus_tag="P5316_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83963.1"
                     /translation="MRKTTLPVLLMGGVLSAGGAALPAQTSVPENVYKGEMVAFPGPW
                     AFQIHRSSIILVTDEELEALADPDRAINLSLGRTPLERSLRQVCEAARAAGQRTLIIA
                     FDHFFAQYRPGQGDKPRRLTPDMPEYIERIAAISKFAATYGLGLELSLLSPLEVGPAY
                     VKETGESGLWMHYRKGLRDPVSGDYSVQLWRQRKWANNKGPIDVADAGVRVFAFREHV
                     IHGTPYRHVDSKDIVEISQTAQVDIWKDTISKPGGAVRIRVHGRGGPPAAAGLDRVLV
                     VQQYSTPEMDYFSDKALPYLNKLTDRYLDAGVKLNGLYADEMHIQQDWGYHTHHDNGE
                     FAVRYVSPAFASRFAAAYGREYHDFAKYMIYFTYGQEDTASDMTAKDHVMHVMAPTPE
                     GIRATALMRARYYRMLQDGVVDLFTAAKRHLENHVGYKLEARAHATWAESPTCDRWRA
                     SHGYEYTADYVGSTMSHQAASACYDYFKWGDFLTGNGNDHAEGGFLDRNYFALALACS
                     TGILNDVPNSYAAHWGMPAEIAQRRHALADAYGDATRPWFGMVQNMEHRDVDVLMLYP
                     INLVAAEERFGNWMTQYGYANYVTPAKLLERGSVANGAIEMAGRRFTTLVTLFEPFPP
                     VKLLEMMRQLAEGGGRVIWSGPPPVLAEDGSTILPTWKDLFGVEYPPTHSEGLMVPGK
                     QVVFEGKLAGIAPMTVLTEMLPDRVYSIAPRPAAQPVARVKNWVVGSHRALDNGGSVT
                     FLGFRPRDDQAASLGYEMRYWFDILSALGAYPSSGKFSGVNDNTEHLSRTNPYLFCRF
                     PNGTVSVAPHLSRLEESWPGGFARNREEDEKILKNLTLPSEHISLKDVKVNGQTVSFE
                     GEQCLAFRADADGRLIAFCGLGCNQITVNGKTTVFADVPMPLVAWAPIEPNCRTEANA
                     VLRVRVHGAGTVRVPAGRLPPEAQVFAEGPTPGSRGPVVESKVENETLILTLKPAETN
                     RWLYVVGGAASKPGR"
     gene            21371..22303
                     /locus_tag="P5316_03055"
     CDS             21371..22303
                     /locus_tag="P5316_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HRR83964.1"
                     /translation="MIRQTTFTIGFMASSAIAVFVSPVGGQSLCSENYQCPYGYFCEK
                     GDGDCWGLGTCQRKPDYCALWVDPVCGCDGRTYSNSCFAAMWGVNVAYPGECGAICQD
                     NSTCRAGTYCRKEPSDCAGFGYCAEIPDGCGTYWDPVCGCDGVTYSNVSCAALYGVNV
                     AFDDPCSSCIDDADCPAGSYCDKPVGDCDGAGVCEPFPAACEPLIEPVCGCDNVTYEN
                     FCEAAIAGVTVQYLGPCMPAISDYDGDGDVDQTDFGFLQRCISGKDVTQTDPYCQPMR
                     MDADEDVDGGDILTFLACLGGPNTLADSDCIDQP"
     gene            22448..23746
                     /locus_tag="P5316_03060"
     CDS             22448..23746
                     /locus_tag="P5316_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR83965.1"
                     /translation="MTADRSESSFSNRTTRRTFIRNAGTVAAAWTIGTGSTAQPARGR
                     VLGANDRINFAMIGCGGEGNALLSMVKILKDQGVNVEIVAVCDIYRPRLDKTAERYKA
                     KAYSNHKELLADSSIDVVGIATPDHIHGYQIIDAIRAGKDIYCEKPITHWRQFELTKR
                     LFHEVSKSDRVFQLGSQYMSDSAWSQARRMIKEGQIGQPVHAEVGFFRVGDWGERGMP
                     IDDPDAEPGPDLDWEAFLGDSPKREFDVSRFFRWRMYWDYAGGPATDLYPHCLSQVIY
                     MLGVKMPSLVVGTGGKLRYEEREVPDTCNILAEYPERISVSMVGTQANDYPGARPRPP
                     ASSPIIRGWEATLTFENEEIVLTPVQGTKKEGKRVRIERPFDHVRHVKNLLDCCRTRE
                     KPEYPVDLAYYTQTPIQMGILSLREGKVARFDSTVEKIVL"
     gene            24050..25984
                     /locus_tag="P5316_03065"
     CDS             24050..25984
                     /locus_tag="P5316_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83966.1"
                     /translation="MPERTGLVRLTGLPDFDCDSIANNAPDQWRWLSLPRSRLVLNPA
                     ASDDFSPLPQRPDLPLHAAYYAGRKPHRLVDLVTVGIGTTDRGPAGHCRHTWYPYKLS
                     FQASWENAEIKISGYDFLADENTIVRVLEVLSGESPSTLRLQGAVHGRLQPDSGVLLA
                     EYEDFFHALMVGKVEGPELTPARLDARPQVCGDGWFLETASGEGRQASSSRPLNSSEA
                     SCYFVSIGFATRAEGADTAVCRAAGVLSGPGLAKRLQDRKAQWSEYLRRVPAPEQFGI
                     VAVDPKGVSPEIHRRFYYGAWAFVISNVLPAMPENDYPYPQTPCGKPSLWNFGASKAP
                     ASAAWESFFGQQMLAYVMPDTAWAAFEGIMAQVDETGRLDGECLPSRKAQTAWILYSL
                     TGELTRLRAVYPAIRRYLLWREQNPRWIYLDHDNPDEKDSSFVDQLLVDIDFAVRIAR
                     EIGENADVAMWQQRREVILKNYREWFFFTDERPPAEYFFTESKRHEPGNLNWVLTGLH
                     IRDLPLDLADELKRLYLQRHRPDRDFCGMSSTKYGTESFIAYGLIEHGVHKQAREFIN
                     AALRDQIRAGEFSENYAAGREGRPPIAWGVKPSLFGAVQMVDFTCLNNGVRLDQGRPV
                     CVTWPPDAAATPAHVERKED"
     gene            25988..26740
                     /locus_tag="P5316_03070"
     CDS             25988..26740
                     /locus_tag="P5316_03070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR83967.1"
                     /translation="MPLEANRIRAILFDYGNTIIPFARPQMDACDNALRAAIERHLGP
                     VDRNRLRELRDADRLAPYNNGYRENDLTEIVTALVRRLFRRDPTPEQIAVLLQARHEA
                     VVAAIEAPSYAAAVLTDLAKRYRLALVSNYPDAEAIRASLRRTGLARFFESVVISGEV
                     GFVKPHPRPFEVCLEQLSLSPSDAVHVGDNWLADVQGAKRLGLQMIWTRQYEPIDSFE
                     VKPGDHQPDLTIQHLTQLTGVFGPRSGQPESS"
     gene            27225..27704
                     /locus_tag="P5316_03075"
     CDS             27225..27704
                     /locus_tag="P5316_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRR83968.1"
                     /translation="MSAKMILKSSAFGPNEAIPRKYTGDGEDKSPPLSWSGAPDGTKE
                     LALIVDDPDAPTPTPWVHWVLYAIPPDTTGLPEGIAPSQRVSEPPGLMQGKNSWGKVG
                     YGGPAPPRGHGRHRYFFKLYALNAPLGVEPGLSKEALLKAMSGHVLAEGELVGTYQR"
     gene            27948..28568
                     /locus_tag="P5316_03080"
     CDS             27948..28568
                     /locus_tag="P5316_03080"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRR83969.1"
                     /translation="MVDTQIARPRDGRKPVKDAKVLQAMRNVPRHVFVPNDYQSRAYG
                     DSPLPIGHGQTISQPYIVALMTEALQLTPEMKVLEIGTGSGYQAAVLAHLTPHVYTIE
                     IVRPLAQSAQRVLLEQGYNSVRCRIGDGYKGWPEESPFDVIIVTCAAENIPEPLWEQL
                     KPGGRIVIPTGSAWGAQELVVVTKTEEGKRKTRHITGVRFVPMTRE"
     gene            29326..32052
                     /locus_tag="P5316_03085"
     CDS             29326..32052
                     /locus_tag="P5316_03085"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRR83970.1"
                     /translation="MVRPSIPVWWWIAPIGAVIALIFAGKFYSEVKRANPGDAEMIEI
                     AGHVREGAMAYLRRQYKVVTIVFLVLAAILAFMAYGLKVQNPIVWFAFLTGGFFSGLC
                     GYLGMRTATMASNRTAAGARESLNKGLVVAFRAGAVMGLVVVGFALIDITGWFLVLYK
                     LFPAIGKEYHLSTITVIMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKIEAGIP
                     EDDPRNPATIADNVGDNVGDVAGMGADLYESYCGSILSTAALGVSAAVALFGSEVTAD
                     GANATIRFLAAPMVLAGIGIILSIAGIYLVRTEEKATMGVLMAALNRGINGSSILIAL
                     AAGLVCYLLLGREFPTTADGYQVCWYGIWGSVVSGLLAGVIIGKATEYYTSYDYSPTR
                     EVSAQGVTGPATIIIGGLAEGMKSTWASLLTIIVAILLAFTFAGGGHEFMLGLYGVGF
                     AAVGMLATLGITLATDAYGPIADNAGGNAEMTGQKPEVRQRTDALDALGNTTAATGKG
                     FAIGSAALTALALLAAYVEEVRFGQIYEARDMVSTYYQAPAPDEAVYLGHGKFACFLS
                     GEVGKVRNSDVEAYMVLNLADLPATGISALKPGETKLKLGKQASKAEGYIRKAVVRDD
                     EGNDHSLELVAARRCSLEQYMQFYNVTLMNPKVLCGLFAGVLLVFLFCSMTMKAVGRA
                     ANRMMIECRRQFEKIRQFLRNQGKDETFVQNPDHWPKRVEVEGQRYPDYATCVAISTA
                     GAQREMIVPALMAIIIPVAVGLVFGVPGVMGLLAGGLTCGFAMAIFMANAGGTWDNAK
                     KLIETFGKLKAKDVLENKELQQRLPAGIRDDLLNKARQAVAAGKPDATIYGKGSDDHK
                     AAVVGDTVGDPFKDTSGPSLNILIKLMSMVSVVFAGLIVKFAPQIGALLGLN"
     gene            complement(32228..33787)
                     /locus_tag="P5316_03090"
     CDS             complement(32228..33787)
                     /locus_tag="P5316_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR83971.1"
                     /translation="MADTAVSVRKTPLRDILAYAMGDGANSLVMNTFYSFAMLYYTKA
                     MGMSGTNAGVAMAITTLWDAITDPLMGHITDNTRSRFGRRHPYMLIGGFAMVLCFYGI
                     WAVPMTFRTPQLLFWYLVVMNVLLRTASTVFAVPFVALGFEICTDYNQRTTLQSVRGA
                     VNMAVNLTGPAILGWAVFLRDRDGEIGSGVTIPDNFKHMGFAFSAIALFFLLYTVFAT
                     RKYAVDSRNNPEVRSNRLGDILRNLTAVLLDPLPRMVFVFIAVLFIAVVLVTSLQMFI
                     YVDFMKFTGVEKTLVHGSTMIAAAIGAILAAPLVRRLDKKPAIFVLLAGACCGNIVLV
                     LLFGTGLVRPGSTPFTVAGQATSVATLVFLVFHVCYHLGATSANTIANSMMADVSEIN
                     RYYTGVLKDGSYSAMLSFVLKMSISFGQLLCGLCLDAIGYDSNAEHQPVEVARRMMFV
                     AFAAGSLITLLALVWIVRYPVNRKYMYEIKAALTGVAPHCRYCGYNLTGNVSGICPEC
                     GAPIASESSPA"
     gene            complement(33933..34472)
                     /locus_tag="P5316_03095"
     CDS             complement(33933..34472)
                     /locus_tag="P5316_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83972.1"
                     /translation="MSRSIMLPLVGILALAVGCVSVKTPDVSVDVMDPVNAYKRHESS
                     SKEPEPAPAPRLRYTPYAATLERVNRQHIRVSEEFTKRDWEDLEDEAQDWMADVRALL
                     GYAPVSHNPEQYRNLAGKLMDAVHDVRTAARAHDAEKCRKALDSADSILNQFTKTFPL
                     TEPADKPVEAKPSRTTKAP"
     gene            34693..>35502
                     /gene="prmC"
                     /locus_tag="P5316_03100"
     CDS             34693..>35502
                     /gene="prmC"
                     /locus_tag="P5316_03100"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRR83973.1"
                     /translation="MADTGEQPTVWTIGRLLDWTAKWFREKQVEGGRLAAELLLARAL
                     NCRKIDLYTRYDSEPSEDQRRAFRELVRQAGDHTPIAYLLGSREFFSLEFQVGPAVLI
                     PRPETEALAQRMIDLCRSTRERRWRILDLGTGSGCVAVAVAKYAPNAVLVASDISEEA
                     LAVARANIEHHGLSDRIRTVVADGFALPAECIPEGGFDAVVSNPPYISEAVYANLPPN
                     IRDHEPRVALVPPCSDGLEMYRRFASEAPAVLGPGGRLLAEIGHDQLAAVQE"
BASE COUNT         6829 a        10741 c        11041 g         6891 t
ORIGIN      
        1 cgaatcgagg tgatcaccag aacctgatcg acgttggcgg cgatcatatg ggttttcttg
       61 ccgtcgcacc ggctcagggc cgttcgacgc gggtacacgt gttcgatcac gccttcgttc
      121 accacccccg cctcgtcggc cacgatagag atggccacct cgtcgccgac caccacgggg
      181 cttcggttcc ggatcagcag cgtccgcagg acgcggcgaa cggtgcacgg ccagattcgg
      241 ccatcgacat ccacgtcgac gaactgcccg cgaaccgcga ctgcgattcc cttaagccaa
      301 ctgccctcgc cgcgactctg atcctggtcg atctcgacaa ccgtccgctt tctggacagg
      361 tcgcccttgg ccttgactgc ctcccgactg aaaacttctg ttccttggtc atcgtccgtg
      421 ggtatttccc agcgcttgcg gcgcgcgggt gtctgtcgat ttcgtcgaaa gtcgacccgc
      481 cgcagtcttc gacctttgcc tgacgactct ccgcccacaa tggatctcca agatcgctcc
      541 agaggcggca cttcccgaga agcagggtac cagcctcgcg gaagattcta acaccgtttg
      601 gacctgaacg tgtaggcgga aatccggttt ttagtctgcg gataccttgc gtcgcgaccc
      661 ctggagggct acactcgctg ttttcggggg agcagccctc aaggcgcccc ccaaagttga
      721 ctgctggcta tccaacagct cgcgccaagc ggttgtgaaa tgagaggtag gtatccgtgg
      781 cagagaactt tgccgaccgt cttttgaagg ccatcaaggc caagggcagc cccacctgta
      841 ttgggatcga tccggtctat tccaagctcc cggccgagat ctcggaaaac aaggagctta
      901 acgacgccac cgactcggag gtagccctgg atgcgattct ggagtactgc cggcggttgg
      961 tgagaatcgt cagcccgctg gtggctgcgg tgaagatcaa tatcgccttt tttgagcgat
     1021 actatgctga cggcgtggat gcgtatttcg atttggtcca ggaggcggat cgagccggcc
     1081 ttatcgtgat tggcgactgc aaacgcggag acatcggctc gaccgccgag atgtatgccg
     1141 ccgccatgct ggccgaaccc gatttcgcca accttgaaga cctgaccggg ccggacgcca
     1201 tcacagtcag cccctatttc ggcctcgacg ggctccagcc gttcatcaac gtagcccgcg
     1261 aggagcaaaa aggggtcttt gtgctggttc ggacgagcaa cccatcggct gccgagttgc
     1321 agactgccag gctcgaaagc ggcatgaccg tggccgagtt tgtcgcccgg caagtcgaga
     1381 gttgggcttc cgatccgact ctggtaggtg aatgcggcta cagtgcaatc ggtgcggttg
     1441 ttggcgcgac ggcccgggac gaaatcgcga cgtttcgagc catgatgccg cattgcatct
     1501 tcctggttcc cggattcggc gctcagggcg gcaccgcctc tgatatcggt gcctgcttca
     1561 aaccggatgg cacgggggct ctggtgaccg cctcccgaag cgttatctat gcctacgacg
     1621 acatgaaata ccttgagcga ttcacctccg aatgggaaaa atgcatcgag caggcctgca
     1681 aggacttcgt cgccgaggtg cgcaacgtag tgccgaggta agtgtaagtt gtcgattgaa
     1741 gaccccttgc cggtcgagga ctccggtcta cctagaaccg cctctgtcgg gtacgttaag
     1801 gggcaaggca ggcgaccttt catgccctcc gccgaccgca ggaaacgcgg ccgaaaagct
     1861 gccttggtgt ccagacaggt ctcctccatc agcccggcag acccgtgaga ctcgagagtg
     1921 cggtaggtgt agcgggggaa cgccggttag caaaacgtcg cccggctgcg tatccgggca
     1981 aggacaaacg ggaaacctcg gtgaagggcg gttgagatgg ctcactatca tcaagcatcc
     2041 tcctgtctgc cgacccgatt gccggcatgt gcgatgcttc tcgtcgtgct tgtcctaggc
     2101 actctctgct cagcagacgc gcaccggttt gtgatcgtgt ccgacctgca ccccacgcat
     2161 gcaatcatcg accaactaga tcgactgacc cgggaggtgc tggagatccg cccggccttc
     2221 gtggtgcagc tcggcgatgt gggcaacgac agctttcccg gcgagagcgt atggcagatg
     2281 aaggtcatcg acgccgcgtt caggaggtgg cgcgaggccg gcatcgagat ccacgttgcg
     2341 gtcggcaatc atgacgtgcc cgccgacaag cctggcgttg cggtgctcaa gaggaactgg
     2401 tttgcctctc agcttccacc ctatcccatg aacgcacaac tggatgccga caaacatccc
     2461 gagatctgga agcgctactg cctcgaaggc aagcactact acgctttcac ctggcgcggc
     2521 gtgcatttcg tgatcatgga ctcggaaaac atcaccgatc accagttggc ctggctggaa
     2581 gcggtactct gccgtcatga agggaatccg tcgcggtatc cgacactggt ctttgcgcat
     2641 gatccgcatg tcatgaccgg tgatcgaggg cacatcagcc ggccgctgta ccgcatactg
     2701 gagaaatgtc cggatggtca ctgcgtccaa gccgtgttcg gcggcgactt tcactacgcg
     2761 cagtattggc cccccgaaga gaatctaggc gcgcacgttt atgcggttcc agcctcggtg
     2821 ctcaacttcg ccattcatca tccccaggaa ccggcgtaca cggagtacat catcgcgacg
     2881 gtgaccgaag atgagattgc tttcgagccg aaacccatcg ggcgtggtgt caacatattc
     2941 aaggacaggg tgtcttatca tccgatcgcg cgccgccgtg ccgattgaga ccaaagaccg
     3001 ctggtgcggt attgcgcggt gtcgcagtga atgaatccga gcagcctatt gagttcgatt
     3061 gatcggttca tgagcatcat cccattcatg gccgcatcat gcacatcgac aagagacaac
     3121 cagcaggggg atgtcgcctt ggcagacgaa tgcaatgctg ctgatggccg gatcctcatc
     3181 gattggtcgg ccgcaggcgg ggcggggcat gagcgagggg gcatcggagt ccatggagga
     3241 catcacgaca ggcgagcgaa ccaggaaggg cggctgcata tacgcaccgt gctgagagcg
     3301 gccgaggaag gagtgcgtat tgccgtacaa gacgttgttc ggatgtgatg cagagaatgg
     3361 gtttttagtc ccttccgtcg caactggcgc gcatgtttct cgatcgcaga gcacttgggt
     3421 tgcggcccgc aaaaaggcgg tccgccttag ctaacgtaat cccaaagcgg cctttggccg
     3481 caaccaaatt gaaaaatcag cgagtaccct acacatcccc ttcggacggg tattgacgtg
     3541 acggctcgaa atctgcccgc tgctccgagg ctcgcacgcg cgtcgttgtg tcgggcgcat
     3601 tggtttgcgg gccactggca agacacatcc cgtccgatct caacgccgcg atatcaaacg
     3661 attacgaaga aggccaaatc atcttcgccg gctgcatctt ggcaaccttt gtctgacggc
     3721 actcggcggg aatgctccat accactacac catgaagcct ccgaggcccc aaaacgtgcg
     3781 cgcaaaatgt acaggagttc cggagtaagg gacgattgtc accgggagct tcaaaccccg
     3841 atatctcgca agctggcgat ttcatctttc gcaactggca atcatacgcc ctggagagac
     3901 ccggttgtct ccaattccca atcccggcac attaaagcgt atcagaaaga gcctggtgtt
     3961 tggtgtcctt ccggcaagcc tgcatgaatc gtgatgcctc tgatatcgtc cgcggccagt
     4021 gcgagcgtgc acggaggacg gcggatgaga accggaacga cgccatcgcc cgcgatcgag
     4081 acttctcgtc ggagcagcgg ccggcctttc atcaggccaa gagcggcccg gtcatggagc
     4141 agttccggac ttggtgcatg cggcagttcg aggaccggct ggtggaaccc aactcggccc
     4201 ccggccaggc catctcccac ctgcggcgac cgtttcgccc gactacgacc tggccccggc
     4261 cctggagcat tgccgatgcg gcatcgtcaa cttctacagc cgcgaagact ggttcgatgc
     4321 cggggtgctg actaaaacct tcggtaccat ggatcgaaag gagacttcca cggccggaag
     4381 gcgaggattc ctcgacgcgg gcggcacact ctgcgtccgc gacggcctga atcaagccgc
     4441 ctggacgccc cagtggcgaa agctcgggca ctggggtggc cacctcggct atgagtcgcg
     4501 aacctgggcc ccggaggtcc tcgcgccgct catcccctcg gcttctggac caccaacagg
     4561 gcagatcgaa ggtgctacag cacggagtgc tcacgcaacg attccagatt atccgagctg
     4621 aacatcacct catgttcatc acgatccgat gctgtccgtc gagcgtggcc ggtatcacag
     4681 gttcgtgtgg ttgaccgtcc agcgcgaagg aagcgatacg agtgccggag ttgcgtatgg
     4741 tgacatccag ggtcatgtcg cgatacttga ctcccgtcag ctgcaaagtc tcaaatcgcg
     4801 gcgggacggt cggttgaaaa cacagttcgt ccggctggaa acgcatgccg aacaggtcgt
     4861 agtagatcat ccgcaggtag ccggtggcac tccaggtctg atgcggctcg ccggcccaca
     4921 ggcgaacctt gccgcggtcg acctgcaagc cgccataggg ctcgccggtg atcggatggt
     4981 agatctcctt gaattcgcgg tggcggtcgg ccatgtcggc caaggcggtc aattccttgt
     5041 cgaacagatc caaccgaccg tgcatcgcgg ccgccgacgc ccagaagccc tggataaacg
     5101 gccagacggt gccgttatgc cggccgaagg tcatgccgtc cggactggca aaccacggga
     5161 acaccggcca gacgcaggga ataccgtatg gcgcgacata ttgattgtcg agcacggccc
     5221 gggcctgttc gggcgacgcg ataccgaaga gaatcgcgaa ggcgtggccg aggccctcca
     5281 tgtactgctc gcaacgtccg gtgggatcgg tgaagtaggc gtagcagcct ttctcgggca
     5341 gccagaggtg ccgattgatt gctcccccga ccgcatcgcc ctgagtccgc aacgactcgt
     5401 tgacctgcgc gtcgagaccg agccgccgcc ccatcaggtc ggcgatccaa tacgcgttgg
     5461 cataaaggca attagtgctc agggccttca tgcggatctt gccgacttcc gggtgagcag
     5521 ccggccagtc gagaatgccc gatgatccgc cagcgtcgtt gtacggttcc ggatacgcgg
     5581 ccacaccgtc gccgtagctg gcgggaccct gaaagagccc catttcggga tcgagctcgg
     5641 tctcttcgaa gtaccgcagc gagttgaccg tcgccgcgtg ggccagttcg aggaactcat
     5701 ggtcgccggt gacgcagtag tagtgccacg cgcccgtggt ccaagcgata gcatcccagt
     5761 actgcccgcc gatgcgaacc aggccgtcat cttcgatcag cgtgctgatc agcgtgttcc
     5821 gggcggcctc aggcgtcagg agggcggctg cgttccacac attgaacgcg cagtcgcgtg
     5881 tccacggcga cttgtaggtc tggccggcca tgatcgaagg cgccggctcg tcgagcaggc
     5941 cgtctttcca tggcttgatg ttgcggcaga gcgtctcgac ggccagatcg tggcagcggt
     6001 cgaaaacgct gattccggac tcaagatgta cgttcggctt ggtcatggga tacggcgtaa
     6061 gcagcgtgcc agtccttatc tctttttgcg acaaaaacct acgactatga ggccgtggag
     6121 gtcgacatgt cgatgccttc gccatatcct cagaacatgt cgtgcacgaa cgtcggcaag
     6181 gtcagcctca actgaaaacg agttggcacg acgacttctt tgccattcgc aagacgaagc
     6241 gtgttccacc atcagggttt cccggcaccg gtatcaatca ctttgaccga tccgtccggc
     6301 agcagcccga cctggtagcg gtatccctgc ccgcgggggt tgtctgccag tgggttggga
     6361 agctggcagt cctcccagtg cagatacgcg gcaataatgc ctagaccgta cagcgtcgtg
     6421 tactgcaaac cgacgttaga cgggtctccg cgcgcgatta gggttcgccc atcgggtagc
     6481 acccaggact catagtcctt ggccagcgac tccagaactt caagaagctc atcgtaccgg
     6541 taatcggaca cttccagaca ataagcagtc acttcggccg cgttccgcgg aacgcaggaa
     6601 ttggccccgt gcttgtacca gtcgccgttg cgggaatgct cgatcatggc gtcggccaat
     6661 tcgcgggtgt gcggcacgtg catgcccatc ttgaaataca ggcgcccgat gaccttgagt
     6721 gttccgccga tgcgtcgcat cagttccgcg gatggcggcc cccaaagccc gtcgtccgga
     6781 tcctggtgcg agaggatctg ctgaacgccc ttttccagat ccgggatcag ttcgacctgg
     6841 ccgctgttga ttgcttcgaa gatctcaacg gcctggaaac cggtgtgcga gccgacaccc
     6901 cagccgccat tctgccagtc aggatcctcg cggcttcgtt ccaggaagac gcgagtctcg
     6961 atcttcttgt cggtccccgt ggtggtccac ttgtacagcg gctcaccgcc aagctgaccg
     7021 atgagaccca cgacgtattt ctcgttgacc tgccgcttgg ggtcacgggg gtccttgaag
     7081 cgcccggtgg ccggatcctg ccagctttgc cagaacttga gggctttggt gcgagcggcg
     7141 tcatcgagcg ggccgacttt atcataaagg ttgtattgcc tgacgagacc ggcaaaggta
     7201 cacgacccca tctcgtcata cggataaccc tggtcaggaa cagtcttggc caagccccag
     7261 acctcgccgt ccttgtacag atgtttctcg atgagaatgt cggcgagccg gtccgggatg
     7321 gcgttgaagc gagcaggcgt ccggccaggc gtttgcttgc gcacgcgttc aagaatccgg
     7381 cgattgcgta aggtcgtttc cgccggccgt gttcgggcag acggttcgct cgtcggggcg
     7441 gctattccag atgccacgac gacgagcagg atcaccagac cgcagcaccg tccgaccgtc
     7501 atatctaaca ggcacattgt tgatcctgac tatcgtccgg tcctccgaag ttgctcaacg
     7561 ggtgggccag tactccgatt cggttctggc agttttcaga taatcctcga tgcgggccag
     7621 aatctccggg tgttccgccg cgatgttgtt cgtttcgccg acgtcgctct tgaggttata
     7681 gagttcgatc tttccgctat tgtgcttgat cgccttccag tcgcccattc ggacggcctg
     7741 gaccaggttg gcctgattga actcccagta catgaagtcg tgctgcttct gcgactcgct
     7801 caacagggcc ggaagcattg aaatcccgtc gatgttcttc ggcggcgaga ttccggcgac
     7861 ctcggccgcg gtgggcaaga aatcccacat tgcccagacc tggtcgctga ccgcgccggc
     7921 tttgatcttt cccggccagc gggcaatcat cggcacgcga atgccgcctt catacaactg
     7981 cccttttgtt ccacgcagcg gaccattgct gctgaaatgc ctgagcgtgc cgccgccgtc
     8041 tggcgcgccg ttatcgctac agaaaaagac cagcgtcctg tcgtccagct tcaggtcttt
     8101 gagcagtttc atcagcttgc cgatgtcggc gtcctggcgc gtgatcatgg ccgcgtagtt
     8161 cttgcacggt tgcggccagt ccctgtctga gtacggcgca tcgctcggga cctcgagctt
     8221 gccgtgggga atcgtgtagg ccagataaag gaagaaaggt ttgtccttgt tgcgttcgac
     8281 gaaggacagg gcttcctgcg tgaacaagtc gtgagagtac tgcttgccct ggccgttgac
     8341 gttaccctcc agcgtgtatt tctcctcgtt cctccacagg taatcgggat agtagctgtg
     8401 cgcatgcgcc tggttcagat aaccgaacca ataatcgaac ccttgcttgt tgggcacgcc
     8461 ggtcgtctcg ggttcaccga ggccccactt gccgacgaca ccggtcgaat agccggcctt
     8521 tttcagcagt tcggcgacgg tgaagtcctc cggacgcagg ggcacgcgct cgaggatcga
     8581 actcttgtta tcgcgcaccc acgcatgtcc ggtgtgcagg ccggtcatca gacagcaacg
     8641 agccggggcg catacggtgc agccggaata gaactgggtg aatcgcatcc cttctgccgc
     8701 cagttggtcg atgttgggcg tcctgatgag cttctggccg taacagccaa ggtcgccgta
     8761 gcccatgtcg tcggccatga catagatgat gttcggtgtt tgagctccga caaccgtgga
     8821 cgcgcaaatc gcaagagcca ggaacgggat caggagtcgc atacgtcacc tctgccatgc
     8881 ttggcatctt cgcaggaccg cgggaatctt cgacctcagg acggacaacc atcttacctc
     8941 gagacttcag ccgagtcagc cccctagccg ctcgcaatca tcccgcagag atctcgcgga
     9001 taccgggcag ccgagactga ttgtcaggtc attgaaacag ggggcaggag cccccgtgct
     9061 tggcgtggta atcctgatga tactcttcgg cctcgtaaaa aggaccagcc ggctcgatgg
     9121 tggtagcgat tctcttccca cgaaaccggt ccttggcagc ctgctcctcg ataaacgcct
     9181 tggcttgctt gagctgctcg tcatcggcgg cgaaaatcgc ggagcgatac tgcgaaccga
     9241 cgtccgggcc ctggcggttg agctgcgtgg ggtcgtggaa cttgaagaac cattcgagga
     9301 gctgccgata ggtcacctgt gccgggtcaa aagtgacgcg gacggtctcg gcatgacccg
     9361 tgcggccgga gcacacctgc ttgtacgttg ggttgactgc cttgccgccc tgatatcccg
     9421 agacggcatc ggcgacgccg ggcacctgct ggaaccggtc ttcaattccc cagaaacagc
     9481 cgcccgcgaa gtaggctgtc tcaagcttga gcggacgcga ttccggcgga agctccttac
     9541 cggcaggata aaacctcagc gagacggaat tcagacaatg gcgaagtccc gtaggcgggg
     9601 gaccatcctc gaaaacgtgc ccgaggtgcg agccgcatct ggcgcagagc gtctccgtgc
     9661 ggaccatgcc gtggctgatg tctcgctgat gtcgaatatg atccgggtcg aacggccgat
     9721 agaagctcgg ccagccggtg ccggattcga acttgctgtc ggacgaatac aacggcaggc
     9781 cgcacagtcg gcacacgtac gtgccttttt ccttgttgtt caataagtgt ccgcaaaacg
     9841 gcgcctcggt acctttttcg agcaaaaccc gccgctctgc tggttcgaga cccttcgcaa
     9901 gttcctggat tctttcatgg gtcaacggcc gaatgtcata accgcttcgt gagatcatgc
     9961 gttgtttgac tccaccggca ggacgggttg ctaacgattg cttttccgag cgccggctgc
    10021 tttgacctgc gacttccatg accagaagcg cgcagcccgc gacagccagc aggacgattg
    10081 caggaagacc gaaagttcgc atactgtgcc cccgcgaaaa gggtcttatg ggcgggccgg
    10141 gccatcgccg ctttcgccag gagcccgtct cccggtgcca tgatgcactt acggccctta
    10201 attctaggac ttgttgcctg ccaaggcccg ttcaaatcgg tcgttggctg cattggcaac
    10261 cgatgaaatc accccccgct ccgagtacgc atcgattccg ggcgcccggt cacattcaga
    10321 acaccctttg cacggccgtc gagtctgccc ggtgactaac gaacccggcg ggatgccgtt
    10381 tgctgctgcg gttcgattcc gttttccagg ccctgggatt tcggtttgaa gccgccagac
    10441 ggcggcttcg ggttccgtac cggcgccggc ggcacggaca cgggttcccc cagagcaaga
    10501 tcgatcttca tcgcgggcgt tctcaacacc caccatcggc gtaaccgaag caccggccga
    10561 acaatcggct ggtagagcag aatcttgtac gacttccggc aaagatagta agcgcccatc
    10621 agcatcgaca tgcagatcct gcgatccagg caacgccgca cgaaccggtt ggtgaccagg
    10681 ccgatgaaac ggtgagtcag ccacggcggt gcgccgaggt tacgcagatg cagcaccagc
    10741 cgcagcatga tggccagata gtccagcttg aagacatgca cgcttcctgt gtaagtgcgg
    10801 tggtacgcct cctcgtggcg ggctccgata atgccgtcgc tcctggctcg ttcataaagc
    10861 ggtgaaccag ggaagagggc caacggaaag atgcgcaggg ccgcagcctt tcggtgatga
    10921 tcggccacaa accgcattgt ttcaatgatg ttggcatcgc tttcgtaggg gttattgacg
    10981 atatagtgat actcggccgg aagtcgatat cgatgcagca ggtccattgc catcgcgatg
    11041 cgatcgaccg gtgtgcggcg gtggtagatg ttgtacaggg tatcacagct gccgctttgg
    11101 atccccatac tcaccagggc gatcggcatc cgtgacaaga cagcgatctt gtcctcggtg
    11161 accagttccg gatgggcgtc gacttccagc ggacgtccga cgcggcgcgt gtatccgtcg
    11221 gcaaacgccg caatctcttc ggccggtcga acaaagaaaa ggtcatccac gatgttgact
    11281 gcgctgatgc ccgggaaacg gcggcacatt tcttcgagct cgcaaaggac gttctcgatt
    11341 gatcggcggc gtacccaaga tcccctccca cggtagatgt tcgcccaggt agtgttgttg
    11401 cagaacgtac agcgataggg acatccgcgg gtggtttcga cgcgcaggcg gtcgagcgcc
    11461 ccgcggagat tcccgggccg ggcgcgaacc aaccgaccgt catcggccac ccagtgactc
    11521 ttcagttcgt agtcgggaaa cggcaaatcg tcgaggttct cgatcagcgg tccaaccgga
    11581 tttcgcacct cgccgcggtt accgaacggg ccgccggccc tgaacgccag tccgccgaga
    11641 tcagtcggat ctcggccgcg gtccatgcgc tcggcgagct gcagcatcac ctgctcgcct
    11701 tcgccgatgc acacgacatc agcgtattca aggcattcgt ccggggccac ggtggcgtga
    11761 gtgccgcccc agatgatcgg aaccttcatc ccagccctgc gcagcgcttc ggtcagaaag
    11821 caggcacggc aaaaggaatt cgtcagcagt cccatgccca cgaggtccgc gtcgcgacaa
    11881 tgctcgatca gtgattcgat caccggcggg gcaaaatccg gcttggccgt gtcgcgcttc
    11941 gatgccatga aaatcatctc gacctcgtgg ccggcggatt tcagggacga tgacacataa
    12001 cgcattccga gggccacggg atgcgtgtag gtggcgatga gaacggttct catggcaggc
    12061 ctccccgaac gaccggtgat cccccatcgt atcgttcaca aatacattcg agatctcgaa
    12121 tatccgttca atgcgtcaag ccacaatcag cgacatcagg tcccgattgc gaggggacat
    12181 tgtgccgctc agaaaacggg gaacaacaac ctgcacacag gacgtacaca ttacgaatac
    12241 acagcggact cccacaagtc agagtggcag gagatgctat tgagcgctct gcaggagccg
    12301 cgtgtcttac agaattatcc tgtatcatac cagatctgca tccgggccgg caagttgccg
    12361 ttgcgcggcg tcttcgcacg cgatggggcc atcagtccag gccgcaggca tattgcggag
    12421 gcccggcctg gggtctacaa tgccaccgga tcgcatcgaa gtcatgaaaa gcattcgcaa
    12481 actgatgcag cagctcgaac aggccctgtc caatccacga gaggagttcg gacgcttcac
    12541 gcgatttgta ctcttccaga tcgagctgtg gcggttttgc gtccgtcgtc tgcgggaaaa
    12601 caacgtcatg gcgatgggtt cggccttgtc gtttcgcacg attttcgcga tgatcccggt
    12661 gctggctttg gcccttctgg tcatgaagag cgtcggcgcg ctggaggaca gccgggccag
    12721 cctgcaccgt ttcctggaag ctttaggttt cacccagatt atcaccgtac aggaaaccag
    12781 gcctgccacg ggtacggctc cgggtgaagt tccgccagga gaggtcatca atgttgctga
    12841 tcggatcgag gaggtcgtcg acaacgtcag cagcaagctc acattccagc gcatcgggcc
    12901 ggtcggcggc gctctgctga tctggaccgc cttgacgttg ctgaccacca tggaggaatc
    12961 actgaatcgc attttcggcg cccgcagtga tcgttccacg cccaagcgcg tcctgttgta
    13021 ttggtcgacc atgaccctag gcccgatcgt gctggcggcg gcgagttatc tcggtcgaca
    13081 aaccatcgag acggtgcgcg acgcccccgg cgcgtcgtgg ctgatcatga ccttgggctg
    13141 gggcggaccg atcgtcgtgg gtgtgatcgt ccttgccctg gtgtatgcct tgcttccgaa
    13201 cacgaccgtc cgattccaag cggcgctagg cggagcggcg gtggccgttc cgctgtggct
    13261 cgtggccaag tggggtttct cactctacgt gaagaacctg gtcgcaaagg gcaacatcta
    13321 cggtgtgctt ggtttgcttc cccttttcct gatgtggctg aacctttcct ggtggatctt
    13381 cctttttggg gcccagttgg cccacactgc cgccaacctt tccgtgatgc gcatggccga
    13441 gcgtgcgggc aatctcgtgc tcgggccgtc cgacctgctg gcggccgcac tggccatcgc
    13501 gagacattac catgccggtc agggaccgat gacggcgggc gccatggcgg gcgtgctcaa
    13561 ccttcctgtc gacgctgtcc agcggctgat cgaccggttg gatagcggcg gtctactgat
    13621 ccgcgtggac cgggaaccgg aaccaggcta cctgctttgc aagccaccga cccggctcgc
    13681 cgtgatcgac gtcatgagca tcggcgaccc tcgcggatcc gggtcatcgc ctgaaggatg
    13741 cgacaagcaa ctcacggcgg tggtgcagga actgcgggac cgcactcgtg cctcattgga
    13801 ggggcttacc ctcgaagact tgctgcgtcg ccacgccgag cccggcccct gcaaggcaac
    13861 cggctgaaca agtccggcat gtaccgactc gaccggaaca tggccgacgt gccggcacga
    13921 acgggcaagt aggccgccga ggtcgcatgg gggaagcggc ccgatacggt gatgcaactc
    13981 gaagcccgag gcaggaggtt cggggccggt tgtccgccat cggatgccgc accggaaatc
    14041 ctgcggagtt ttgcggccgc atgattcagg actttatgct ggaagctgtc cgtgggtggt
    14101 ttcctgtttt ggctggccga tcttatcggc tgcccgtgct atcggacggt ctgtcaacct
    14161 cattggccgc cacttacgaa ccgataaagg cagcgggagc attgcgcgcg atgggcgaac
    14221 ggcctaacat cctggaaatc ctgaagaacg agagcgggcc tgccgcggat tatgctctgc
    14281 tgaccgcact gcgcgaagtc ggcgcggcga ccgcccaggc catcgtcgac ctgttgctgc
    14341 ttcgcaagca gaaagccggg ctgtgtggcc tcattgaact ctttcacgag ttggacgagc
    14401 cgatctgccg aacgatcgtc gagcacgtgg atgagctgtt tccggccttg cgcctggcgg
    14461 cccaatccaa ggacgaccag gttcggctga atgtcctcga gatcatcagt cgcggatacg
    14521 cctaccgggc ggcatacctg gtcgacaccg caatgcacga tcgagttgcc cgggttcgcg
    14581 aggctgcggc ggacaccttg tacgccctgg ccgaccagtt gctcaagacc tcgccgatcc
    14641 ccgatctgca ccacgacaag cttgatccgg cgcgcgctcg cggtctgaac gttgacctgg
    14701 aagactacat ggaagaccgt cgtcaggtca ttggtgcaat cgagggcagc ctggccagtt
    14761 acaacatcca cctgcaaccc aaggtcatcg agacagcgat gtggtttgtc gatgaactgg
    14821 atcagaagtt ctggtcgatc gtcggcgtcc ccggcagccg ggcggcccat gcggcgatca
    14881 gcgtcatcgt gggcgtgaag agcccgcggc tggtgccgtt ctgtatcacc ggtctgcact
    14941 acagcacgtt tcgggcgccg ttgtccaagg tcctcggcct ttgcacggac atggcgttca
    15001 tcgaggagtg gatacggcag tcgtggcgtc tgggtgagcc caagatccac cgggccatgg
    15061 cgagcattaa ggagctggcc gtcttgaagc gcaaggcggt ggaactgatg caactgccgc
    15121 cggccctgat gcgccacctg ccgaggtgga tcatggcgac cggactgcct gagcaggtga
    15181 agctggattt cctgaagaac ctttaccgtc gcggcgacga gcagatgcgt cggtccacgg
    15241 ttaagagcat gttgccgtgt gcaggcgagc gagtgatctc cctgctgaag gcgatctcac
    15301 gggacaatga cgagcggacc gccgctgtgg cccggtttga actggctcac cgattccccc
    15361 tcgagtatcc tccatccgac ttgctggaac ctccgaaggc cgcggcggct ccgtccggcc
    15421 aggtctgggg acgacccggc tcggatctga cgttcgagaa gtactgggac atttacgaca
    15481 cggagcccga cccgcagcgt cgggagatcg gcctgcgggt gttcgatgga gacgcctcgg
    15541 ccgtgcgtct gctggccagg cagatgaacg ctgccgagcc gagcgaccgt ctcaaggccc
    15601 tcagaatagc caccctcctt ggtcttggag agaaattcgt ccagcagatt taccgtttga
    15661 gccatgaccc gaaccctgag gttcgcagcg cggcggtttc cgcactgggt agactgcccg
    15721 gtccggtgag tcagcatctc ctcaggacgg cgctacagga cagcgacgcg cgcgttcagg
    15781 ccaacgccgt ggaagcgatc gagcacaaag gcggcgagca gatcaccgag gatctcctgc
    15841 ccaagctggc ctcgcccgac aatcgcgttc gggccaacgc ggtcaaggcc ctgctcaagc
    15901 tcggcgtgcg ggaggcggcc attaccctcc tgaggatgct ggatgatcca aaccgggcac
    15961 agcggatcag cgccctgtgg ctcattgaga acatgggact gttcatcctg gcctcgcggg
    16021 ttatgaagct ggcgcaagcc gatcaggaca acctggtgcg cagccgagcc aggaagctga
    16081 ccgggcagat caccggacaa ctgccggtgg gagcagcgca atgagtatga gtgccggcag
    16141 atccgttttc gacttgcttg ccaaggccgg gctgccgctt gccgagggaa gctggtatga
    16201 atcggtgcaa caccggttcc tcttcggcga accggaactg attcgcggct tgtttaccgc
    16261 ggcgctcgcg atgggtttgg tgctcttggt catcggcatg tcgtaccggt ggcagaagcg
    16321 tcgccaacaa ccggcgccga cccagccgat ggcccttttc agacgcgtgc tgtcaaagat
    16381 gggattgacg gtgatcgagc gatggttgct gtggcgactg gccaggaaca gcggacttga
    16441 acacccgacg gcgttgctga tttcgtcacg cctgtacgac ggggcggtcg accgctactg
    16501 cgcgggcagc ggcctgtttt tcgcccgtgc cggcaaggcg gcgagctttg ccgcgatccg
    16561 ccggcggatc ttcggggaaa agaacggata atcaggtcct gcgatcttcg gcccggcgtt
    16621 cctcacgtgt acaatacttt tcatgcgagt ggtcgtgacc atcctgtcgc atgccgtggc
    16681 cttcgccatc gagagctgcg ttcgcttcta ccaggtggcc ttgcgcccgc tgctgatcgg
    16741 ctcgtgcaag ttcgtgccgt cgtgcagcga ctacttcatc gaggcggtgc acgagtgggg
    16801 accgattcgc ggctcgtggc tgggcatcaa gcgtctcctt cgttgccggc cgtttaccat
    16861 gggcggcatc gacccggtgc cgaggcggaa ccggccatga accgggtcac tgacgatcat
    16921 gccgatccgg agacggcgcg cgtttagccc cctgttgaat cggtgggtag accaccgccg
    16981 tcagcaagag gggccgacga gaagcgattc aattcttcgg tgatcgcctc acgcaacttg
    17041 tggaaaaacg cgatgtacag cggtagttcg cccaagaata ggatggtcgc cataacgaac
    17101 agcgtcgatc ccgggcagat gacgatgctg atatcccagg cttcttccag gatgagcatg
    17161 aacaattgaa taacgagcgt gctcatgcac agggcggttg cccagactcc gaccgtggcc
    17221 gtcttccgaa gccggtccgt cgggtatcgg aaggcgagct ggcagcagta gtagctgaag
    17281 gatatcggta tgctccccgc gcatgcgaca gccagcatgg ccacggatgc agagagccac
    17341 caaagcgggg cgagcatgat cgcgcagatg atcagcatca cggtgccaag aatgcccacg
    17401 aagaagacaa ctctcgtcct cgacaggccc tgaccgaccc aggagggcgg ccttgccccg
    17461 atcacaacga acgtcccgat cgccagcatg aaaaggccga ggagcagaca gggcatgaac
    17521 acggccgtca gcagcccgaa accagaaccg ccggtcatca agggtatgag aattgtgatc
    17581 actgcaaccg ataggaatga tatacccagg tagagaacat caaggccgcc tttgagttcg
    17641 gttatccaat aaagatggat ggcattggga tccgactcgc gaatcgaagc cgcgacttcc
    17701 tttccgcatt cggggcagcg gccatcggca cgcagggtgc ggagattgta ctcgcagccg
    17761 acgcacggca ggtcgcaagc tatgcggtcg gtggccgggt ccaccgccgg ccgcggcata
    17821 cctggaccac ccggcggctt cgggttggat tgcacgggct tcgggtccga ttctgtcacg
    17881 tctcggcgac ctttccacag tcagtcggct tcaccgttcc acctgcaaca ccggacagac
    17941 agggcatcat cgtcgggtca ggtgggcaat cacctgcccg gcttgctggc cgccccaccc
    18001 acgacgtaca gccaacgatt ggtttccgcc ggtttcagtg tgaggatcag cgtctcgttt
    18061 tccaccttgc tttcgacaac tgggcctcgg cttccggggg tcgggccttc ggcgaagact
    18121 tgcgcctcgg gcggcaggcg gccggccgga acgcgaacgg tgcccgcccc atgcactcgc
    18181 actcgcagga ccgcgttggc ctcagtccgg cagttcggct cgatcggtgc ccaggctacc
    18241 agcggcatgg gcacatcggc gaatacggtc gtcttgccgt tgacggtgat ctggttgcat
    18301 ccgaggccgc agaaggcgat cagccggccg tcggcgtcgg cgcgaaacgc gaggcactgc
    18361 tcgccctcga agctcaccgt ctgaccgttg accttgacgt ctttgagcga gatgtgctcg
    18421 gacggcaggg tcaggttctt gagaatcttc tcatcctcct cgcgattgcg ggcaaagcct
    18481 cccggccagc tttcctcaag ccgtgaaagg tgcggcgcaa cgctcaccgt cccatttggg
    18541 aaccggcaga acaggtaggg gtttgttcgc gagaggtgtt cggtgttgtc gttgacaccc
    18601 gagaacttgc cgcttgacgg gtaggctccg agcgcgctga gaatgtcgaa ccagtatcgc
    18661 atctcgtagc cgagactggc ggcctggtcg tcgcgcgggc ggaaacccag aaacgtcaca
    18721 ctgccgccgt tgtcgagcgc cctgtgcgag ccgaccaccc aattcttgac gcgggcaacc
    18781 ggttgcgcgg ccgggcgagg tgcgatcgag tatacccgat ccggcagcat ctcggtcaga
    18841 acggtcatcg gggcaatgcc ggccagcttg ccctcaaaaa cgacttgttt tccgggcacc
    18901 atgaggcctt cgctgtgcgt cggcgggtac tcgacgccga acagatcctt ccatgtgggc
    18961 aagatcgtcg agccgtcctc ggccagcact ggcggcgggc cggaccagat cactcggccg
    19021 cccccctcgg ccagttgccg catcatctcc agtagcttga ccggtgggaa cggctcgaaa
    19081 agcgttacca gagtggtgaa ccgtcttccg gccatctcga tcgcgccgtt ggcgacgctg
    19141 ccgcgttcca gcagtttggc cggcgtgaca tagttggcgt agccgtactg cgtcatccag
    19201 ttgccgaacc gctcctcggc cgccaccaga ttgatcgggt agagcatgag cacgtcgacg
    19261 tcgcggtgct ccatgttctg caccatgccg aaccatggac gggtggcgtc gccatacgca
    19321 tcggccaacg catgccgtcg ttgggcgatc tcagcgggca tgccccagtg ggcggcatat
    19381 gaattcggta cgtcgttgag gatgcccgtc gagcaggcca gcgccagggc aaagtagttg
    19441 cggtcgagga agccgccctc ggcgtggtca ttcccgttgc cggtgaggaa gtcgccccac
    19501 ttgaagtagt cgtagcaggc ggacgcggcc tggtgtgaca tggtcgagcc gacgtagtcg
    19561 gcggtatact cgtaaccatg gctggcccgc cagcggtcgc acgtcgggct ctcggcccag
    19621 gtcgcgtggg cacgggcctc aagcttgtat ccgacatggt tttcgaggtg tcgcttggcg
    19681 gcggtgaaca ggtcgaccac gccgtcctgc agcatcctgt agtatcgggc ccgcatcaat
    19741 gcggtggcgc gtattccctc cggcgttggt gccatcacgt gcatgacgtg atccttggcg
    19801 gtcatgtcgc tggccgtgtc ttcctggccg taggtgaagt agatcatgta tttggcgaag
    19861 tcgtggtatt cccggccgta tgcggccgcg aatcgactcg caaacgcggg gctgacgtag
    19921 cgcaccgcga actctccatt gtcgtggtgg gtgtggtagc cccaatcctg ctggatgtgc
    19981 atctcgtcgg cgtagagccc gttgagtttc acgcccgcgt cgaggtagcg atcggtcagt
    20041 ttgttgaggt atggcagggc cttatcgctg aagtagtcca tctccggcgt gctgtactgt
    20101 tggacgacga gaacgcgatc gagcccggca gccgcaggcg gcccaccccg cccgtgaacg
    20161 cggatgcgaa ccgcaccacc cggcttgctg atcgtgtcct tccagatgtc aacctgggcg
    20221 gtctgcgaga tctccacaat gtccttcgaa tcgacgtggc ggtaaggcgt gccgtgtatg
    20281 acgtgctcgc gaaaggcgaa cactcgcacg ccggcgtccg caacgtcgat cggccccttg
    20341 ttgttggccc acttgcgctg gcgccagagt tgtacgctgt agtcgccgct gaccggatct
    20401 cgcaggcctt tgcgatagtg catccacagg cctgattcgc cggtctcttt cacgtaggcc
    20461 gggccgacct ccagcgggct gagcaggctg agttcgagtc ccagcccgta tgtggccgca
    20521 aacttgctga tggccgcgat gcgttcgata tactcgggca tgtcgggcgt cagtcggcgc
    20581 ggcttatcgc cctggccggg gcggtactgg gcgaagaaat ggtcaaaggc aatgatcagc
    20641 gtgcgttgtc ccgccgcccg agccgcttcg cacacctgtc gcaggcttcg ctccagcgga
    20701 gtccggccga ggctgaggtt gatcgccctg tccggatcgg ccagagcctc caactcctca
    20761 tcggtgacca gaatgatcga cgaccggtgg atctgaaacg cccaagggcc gggaaaagca
    20821 accatctcgc ccttgtacac gttctcggga acgcttgttt gcgccggcag ggcggcaccg
    20881 cccgccgaga gaaccccgcc catcagcagc actggcaatg tcgtctttct cacgcgtcac
    20941 tcctgtgtct tgtgcattaa ggccggtccc ggatctttgc atcctgcggg cctcacgata
    21001 gtcgcactcc gggccgcgcg gagaccggtt tcttcccatc tacagggaga ccctgcctga
    21061 gtccgatgac ctctgtcgcc ctcgcgggac agtctacctc ttaagctcat tgatgcataa
    21121 ccgacagtct tgcggtgtgg ctgggccaat cggcgtttgg gtgaggatcg ggacggcaaa
    21181 gcgacccagc gggcacagct ccactgcctt gtcaacaagg gccttgcgaa gcgcgtcgac
    21241 gcggtcgtgg gaaagcttgg gaacgcagga tcttgccagc gagagcgccc tgctataaag
    21301 tgttgatacg agttcaacca tctttcatgc ggctgcgtct catccgtcct ctcagaccag
    21361 gagccgtcag atgatcaggc aaacaacctt cacaatcggc ttcatggcat cgtctgcaat
    21421 cgccgtcttc gtttcgcccg tgggcggaca atccctttgt tctgaaaact accagtgtcc
    21481 atacggctac ttctgcgaga agggcgatgg cgattgctgg ggtctgggaa catgtcagcg
    21541 caagccggat tactgcgcct tgtgggtcga ccccgtgtgc ggctgcgatg gccgcacgta
    21601 ctcaaactcc tgttttgcgg cgatgtgggg agtgaatgtt gcctaccccg gcgaatgtgg
    21661 agccatctgc caggacaact cgacctgcag ggcaggtaca tactgtagaa aagagcccag
    21721 tgattgcgca ggcttcggat attgcgccga gattccagac ggctgcggga cctattggga
    21781 cccggtttgc ggctgtgatg gtgtgactta ctccaacgtc agttgcgccg ctctttacgg
    21841 cgtcaacgtc gcttttgacg atccctgctc atcatgtatc gacgatgcgg attgtccggc
    21901 cggcagttac tgcgataagc ccgtcggaga ctgcgacggc gcaggtgtat gcgagccttt
    21961 ccccgcggcc tgcgaacctt tgatcgaacc ggtctgcggg tgcgacaacg tcacttacga
    22021 aaacttctgc gaagccgcga tcgcaggcgt gaccgtccag taccttggcc cttgcatgcc
    22081 cgcgatctcc gactacgacg gcgatggcga cgtcgaccag acggatttcg gttttctcca
    22141 gcgctgcatc agcggaaaag acgtgaccca gacggacccg tactgccaac cgatgagaat
    22201 ggatgcggat gaggacgtgg acggcggcga catcctgacc ttcctggcct gccttggcgg
    22261 ccccaatacg cttgctgatt ccgactgtat cgaccagccg tgacgttgcc ggctcagccg
    22321 aataagttca tgcccatgcc cccatgacgg cccatcgatc atcggctaca atccggtttc
    22381 ggggcattgc tcccgtgcaa cggcaaccgc caaagctgag ctcttgcgcc cagtaggagg
    22441 actaccgatg accgcagacc ggtccgaatc gtcattctcg aatcgcacaa ctcgtcgcac
    22501 cttcattcgc aacgccggga cggtcgcggc cgcatggacc atcggcaccg gctcgaccgc
    22561 ccagccggca cgaggccgcg tgctgggggc caacgatcga atcaacttcg ccatgatcgg
    22621 ctgcggcggc gagggcaacg ccctgctgtc gatggtcaag atcctgaagg accagggcgt
    22681 caacgtcgag attgtggccg tgtgcgacat ctatcggccg cgactcgata agaccgccga
    22741 gcgttacaag gccaaggcgt actcgaatca taaggaactg ctcgcagact catccatcga
    22801 tgtcgtcggc atcgccacgc ctgaccacat tcacggctat cagatcattg acgccatccg
    22861 ggccggcaag gacatctact gcgagaaacc catcacccac tggcggcaat tcgagctgac
    22921 caagcggttg ttccacgagg tcagcaagtc cgaccgtgtt tttcagctcg gctcgcagta
    22981 catgtccgat tcggcctggt cgcaggcccg ccggatgatc aaggaaggac agatcggtca
    23041 gccggtccat gccgaggtag gattcttccg cgtgggtgac tggggcgagc ggggaatgcc
    23101 catcgacgac cccgacgccg aacccggccc ggacctggac tgggaggcgt tcctgggcga
    23161 ctcgccgaag cgagagtttg acgtcagccg ctttttccgc tggcggatgt attgggacta
    23221 cgcgggagga ccggccaccg acctctatcc gcactgcctc agccaggtga tttacatgct
    23281 tggtgtaaag atgcccagcc tggtggtcgg cacgggcggc aagctgcggt atgaggaacg
    23341 cgaggtaccc gacacctgca acattctggc cgagtatccg gagaggatct ccgtatcaat
    23401 ggtcggcacg caagccaatg actaccccgg agccaggccc cgcccaccag catccagccc
    23461 gatcatccgc ggctgggagg ccacgctgac ctttgaaaac gaggaaatcg tgctgacccc
    23521 cgtccagggc accaagaagg agggcaagcg ggtcagaatc gaacggccgt ttgaccacgt
    23581 gcggcacgtc aagaacctgc tcgactgctg ccggacgcgc gaaaagcccg aataccccgt
    23641 ggatctcgcc tactacacgc aaacgccgat acagatgggc attctttcgc tgcgcgaggg
    23701 caaggtggca agattcgact ccacggtgga gaagatcgtc ctttagaacc ctacggccag
    23761 atatctgtgt ggcttgcgcg catgtttctg gatcgcagag cgcttgggtt gcggcccgca
    23821 aaaaggcggt ccgctcaggc tgctcagttg ttggattgcc ggtgcaggta tccggtgagg
    23881 aacctgcaag aaaaccttgc ccggaccgga ctatattcgg aatcgtgtga tggtcagagt
    23941 tgctcgaaga agacgcaggg agccggcccg gcggcaggtg tctcacgctg tcctctgggt
    24001 ggtcttgccc gcattgaatg gtgctgtcat gtctgccgag aaacctgctt tgcctgaaag
    24061 aacaggtctg gttcgtctga ccggccttcc tgatttcgat tgcgattcca tcgccaataa
    24121 tgccccggac caatggagat ggctgagcct gcctcggagc cggctggtct tgaatccggc
    24181 ggcctccgat gacttttcac ccctgccgca gcgacccgat ctgcccttgc atgcggcgta
    24241 ttacgccggc cgaaaacccc accggctggt tgatcttgtg acagtgggta tcgggacgac
    24301 cgatcgcggt cctgccgggc attgccgtca cacctggtac ccgtacaagc tgtcatttca
    24361 ggcgagctgg gagaacgcgg agatcaagat atccggctac gacttcctgg cggacgaaaa
    24421 caccatagtt cgcgttcttg aggtcttgtc cggcgaatcg cccagcacct tgagacttca
    24481 aggcgcggta catggccgct tgcagccgga ctccggagtg ctgctcgccg aatacgagga
    24541 cttcttccac gccttgatgg tgggaaaagt cgagggaccg gagctgacgc cggcaagact
    24601 cgatgcccga ccgcaagtct gcggagatgg atggttcttg gagacagcct ccggcgaagg
    24661 tcggcaggcg tccagttccc gaccgctcaa cagcagcgag gcgtcctgct atttcgtgtc
    24721 catcggtttt gccacgcggg ccgaaggcgc ggacactgca gtttgtcggg ccgccggagt
    24781 gttgtccggc cccggtctgg ccaagcggtt gcaggaccgg aaggcacagt ggagcgagta
    24841 cttgagacgc gtgccggccc cggagcaatt cgggattgtc gccgtggatc ccaaaggcgt
    24901 aagtcccgag atccatcggc gcttctacta cggcgcgtgg gcgttcgtga tttcaaacgt
    24961 tttgccggcc atgcccgaga acgactaccc ctatccgcaa actccctgcg gcaagccgtc
    25021 gctctggaat ttcggggcat ccaaggctcc ggcatcggcc gcatgggaaa gcttcttcgg
    25081 ccagcagatg ctcgcgtatg tcatgcccga caccgcgtgg gcagcgttcg agggaatcat
    25141 ggcccaggtt gatgagaccg gccggctcga cggcgagtgc ctgccctcgc gaaaggccca
    25201 gaccgcatgg atcctctaca gtctgaccgg cgagctgacc cggctgcgag ccgtgtaccc
    25261 ggcgattcga cgatacctgc tctggcgaga acagaacccg cgatggatct acctcgacca
    25321 cgacaacccc gacgagaagg attcctcgtt cgtcgatcag cttctggtgg acatcgattt
    25381 cgccgtgcga atcgcccgcg agatcggtga gaacgcggac gtcgccatgt ggcagcagcg
    25441 gcgcgaggtt atcctgaaga actaccgcga gtggttcttc ttcaccgacg agcgtcctcc
    25501 tgccgagtac ttcttcaccg agtccaaaag acatgagccg ggaaacctca actgggtcct
    25561 taccggcctg cacatccgcg acctgccgct cgacctggca gacgagctga agcggcttta
    25621 tcttcagcgg cacaggccgg accgcgactt ctgcgggatg agctcgacga aatacggcac
    25681 agagagtttc atcgcctatg gcctgatcga acacggcgtg cacaagcaag cccgcgagtt
    25741 catcaatgcc gctctccggg accagattcg ggcgggcgag ttctcggaaa actatgccgc
    25801 cggccgcgaa ggccgcccgc cgatcgcctg gggtgtcaag ccgtctttgt tcggcgcggt
    25861 ccagatggta gatttcacat gcctcaacaa cggggtccga ctcgaccagg gccggcctgt
    25921 gtgtgtcact tggccgcccg atgccgccgc aaccccggca catgttgaga ggaaggaaga
    25981 ctgacgaatg ccgctggaag caaaccggat cagggccata ctgttcgact acggcaacac
    26041 gatcattccg ttcgcacggc cgcagatgga cgcgtgcgat aacgccctgc gcgcggcgat
    26101 agagaggcac ctcgggccgg tggatcgcaa caggctacga gagcttcgcg acgccgaccg
    26161 gctcgccccg tacaacaacg gctaccggga aaatgatctg accgaaatcg tgaccgccct
    26221 tgttcgtcgg ttgttccgtc gcgacccgac tccggaacag attgccgttt tgctgcaggc
    26281 ccgccatgag gccgtggtgg cggccatcga agcaccctcg tatgcggctg cagtactgac
    26341 cgatcttgca aaacgctatc ggctggcgct ggtctccaac taccccgacg cagaggcgat
    26401 ccgcgcaagc cttcggcgca cgggccttgc acgattcttc gagtcggtcg tgatatcagg
    26461 agaggtcggc ttcgtaaaac cgcatccccg gccgttcgag gtctgtctcg aacagctcag
    26521 tctgtcgcct tccgacgcgg ttcacgtcgg cgacaactgg ctcgccgacg tccaaggagc
    26581 caagcggctc ggcctgcaaa tgatctggac ccgccagtat gaacccatcg acagctttga
    26641 ggtcaagccc ggtgaccacc agcccgacct gacgattcag cacctgaccc aattgacggg
    26701 cgttttcgga ccgaggtccg gacaaccaga atcgtcgtga ccaatgacgg aaacggcgag
    26761 cgatcatgtt tttcgaaggc ggtcttcaac cgcaacccga acgtgtgcct gattcgcgca
    26821 tgttgcgccg taagaatcaa acgggccggc actcgaacgt ccgattctca ctccagagat
    26881 ccagagggcc acaggggtgc agactcggga cgatgtaggt gccggtttgg cggcctgcga
    26941 gatcgtagac aaacgccttt gtttcacgca tgacgtgggc cggcaggtcc gcagacctat
    27001 agatcagggt gatccagttg gctgtccatt tgcgagacgg ccgtgatata atacggcgtc
    27061 tgccagtctt ggattgaaag cgaggtgcgg cgatgtgtcg gatacgacgc tactcacggc
    27121 ttctcgcgat tctgcggttc ttgttgatcg cggttcttcc ggcctgcgat tcgaccccgc
    27181 cggccggcac cccaccggcg tcccagccag aggaggaggc caccatgtcg gccaaaatga
    27241 tcctgaaaag ctcggccttc ggacccaacg aggctattcc gcggaaatac accggcgacg
    27301 gcgaggacaa gtcgcctccg ctttcttggt cgggcgcacc ggatggaaca aaagagctcg
    27361 ccctgatcgt ggacgatccg gacgctccga ctccgacgcc gtgggtgcat tgggttctgt
    27421 atgccatccc acctgatacg accggcctgc cggaagggat cgcaccttcg cagcgggtat
    27481 cggagccgcc gggtctgatg cagggcaaaa actcctgggg caaggtaggc tacggcggtc
    27541 cggcgccgcc aaggggccat ggtcgtcatc ggtacttttt caagctttat gctctgaatg
    27601 cgccgcttgg tgtcgagccc ggtctatcca aagaggcctt gctcaaggcg atgtcaggcc
    27661 atgtgctggc cgaaggcgaa cttgttggaa cttaccagag gtgaacgatg atcccgcgcg
    27721 cgaaccgacc atgtaaggtt gcgtcggccg ccgggctact ggttgttgtc atgctcatgt
    27781 tcccgtccgg ctgcggatca caacccgacc ccatcgacga agcggtccgc ctggcggcga
    27841 gcgcatctgc tcctgcgagc gagccgctgg taacatcgca gccggctggg ccgtttcgcc
    27901 caccggaacc gccgggggcc cgcgatcgaa tcgaagagcg aaaccgcatg gtcgacacac
    27961 agatcgcgcg gccacgtgac ggacgcaagc cggtcaaaga cgccaaggtc ttgcaggcca
    28021 tgcgtaatgt gccccgccac gtcttcgtcc caaatgatta ccagagcagg gcttacggcg
    28081 attcgccgct acccatcggg cacggtcaga cgatctcgca gccctatatc gttgccctga
    28141 tgaccgaggc tctgcaattg acgccggaga tgaaggtcct cgagatcggc acgggctcgg
    28201 gctatcaggc ggcagtcttg gcccatttga cgccccacgt ctacacgatc gagattgtac
    28261 gaccgctagc ccagagtgct cagcgggtgc tactcgaaca gggctacaac tcggttcggt
    28321 gccgaatcgg cgacggatac aagggctggc ccgaggagtc gcctttcgac gtgataatcg
    28381 tcacctgtgc cgctgagaac attcccgagc ccctctggga acagctcaag ccgggagggc
    28441 ggatcgtcat tccaacaggg tcggcttggg gtgctcagga actggtggtg gtcacgaaaa
    28501 cggaagaagg caagaggaaa acccgacaca tcaccggcgt gcggttcgtg cccatgacgc
    28561 gggagtgagt cctaaagacc gcccttcccg gactggagcg cgtgaacccc gcagcttgtc
    28621 aagtctgctg caaagtcatg agcagcaagc acttagcgtc tctaattgct tatcgagtgg
    28681 gctgcggggg gctaatcgct atcgtttgag gatccagggg agtggacttt gtggccatca
    28741 agatgcagtc gctgggccct tcgccggctc cctcttcagt aagggcgatg gcgaaccggc
    28801 agggtcgcag ggcgctgggc agcgcggcgg cggggtgccg gcggcgacga agcgcgaggc
    28861 ggtttccagt gggcgggctt tatgctatac tgcccgcctt ttaagggccc gggccggcta
    28921 cttggtgaag cacgaaaacg caaaccggtg tcccgtgtcg gtcgaacact ggaaaggccc
    28981 cttcagaccc gcagggctaa cgctcgtgag acatgggggc cgattattcc ttttgagccg
    29041 agcggcttcg cggcgcggtg cgaagcctgc gaggcggcaa gcgacgacaa gggctcggca
    29101 gcggcgcgag gcgagccccg tcagtggctt cagacgacaa tccacctaac cgcaaggtgt
    29161 tcaagaacga cagcgtcaat ggttctacga cactcttccc ataggagaag cgacaagatg
    29221 acagcgttgc attcgcgtag aggctggatg atgtgggtgt ttgcgatcgg gcttgtctgc
    29281 atggttgttg cgcccgtgct ggcccaggag ggcgacgaag gccccatggt tcggccgagc
    29341 attcccgtct ggtggtggat cgctccgatc ggcgcggtta ttgccttgat cttcgccggc
    29401 aagttttaca gcgaagtaaa gagggcaaac cccggcgacg ccgaaatgat cgagatcgcc
    29461 ggacacgtgc gtgaaggcgc catggcctac ctgaggcgcc aatacaaggt cgtgaccatc
    29521 gtattccttg tgcttgcggc gattctggca ttcatggcct atggtctgaa ggtgcaaaac
    29581 cccatcgtct ggttcgcgtt tctgaccggc ggcttcttca gcggcctgtg cgggtacctg
    29641 ggcatgcgaa ccgccacaat ggccagcaac cggacggcgg ccggggcacg agagagtctc
    29701 aacaagggac tggtggttgc ctttcgggcc ggtgcggtca tgggactggt ggtcgtcggc
    29761 ttcgccctga ttgacatcac cggctggttc ctcgttctgt acaagctgtt cccggccatc
    29821 ggcaaggagt accacctgtc aaccattacc gtcatcatgc tcacgttcgg catgggtgct
    29881 tcgacacagg cgctctttgc tcgtgtgggc ggcggtatct acaccaaggc ggccgacgtc
    29941 ggcgccgacc tggtcggcaa gatcgaggcc ggtattcccg aagacgatcc gcgtaacccg
    30001 gccaccattg ccgacaacgt cggcgacaac gttggtgacg tggcgggcat gggcgccgac
    30061 ctctacgagt cctactgcgg ctccattctc tccacggccg cgttgggtgt ctcggccgcg
    30121 gtggccctgt tcggctccga ggtcaccgcc gacggcgcca acgccaccat ccgcttcctg
    30181 gccgcgccga tggttctggc aggtatcggc atcattctct cgatcgccgg catttacctg
    30241 gttcggacgg aagaaaaggc caccatgggc gtgctcatgg cggccctgaa ccgtggcatc
    30301 aacggaagca gcattctcat cgccctggcc gccggcttgg tgtgctacct gctgctgggt
    30361 cgcgagttcc ctacgaccgc agacggttac caggtatgct ggtacggcat ctggggcagt
    30421 gtggtcagcg gtctgctggc cggcgtgatt atcggaaagg cgaccgagta ctacaccagc
    30481 tacgattaca gtcccacccg tgaagtcagc gcccagggcg tcaccggccc ggcgacgatc
    30541 atcatcggcg gcctggccga gggcatgaag agcacctggg cctcgcttct gaccatcatc
    30601 gtggcgattc tactggcctt cacgttcgcc ggcggcgggc atgagttcat gctgggcctt
    30661 tacggcgtcg ggtttgccgc agtcggcatg ctggcgacgc tgggcatcac gctggcgacg
    30721 gacgcctacg gtccgattgc agacaacgcc ggcggcaacg ccgagatgac cggtcaaaag
    30781 cctgaggtgc ggcagcgcac cgacgccctc gacgcgctgg gcaacaccac tgccgcgacc
    30841 ggcaagggct ttgcgatcgg ttcggcggcc ctgacagccc tggcgctgct ggccgcatat
    30901 gtcgaggaag tccgcttcgg acagatctac gaggcccgcg acatggtctc gacctattac
    30961 caggcgccgg cgcccgatga ggccgtttat ctcggccacg gcaagtttgc atgcttcctg
    31021 agcggcgagg tcgggaaggt tcgcaacagc gacgttgagg cttacatggt gctgaatctt
    31081 gccgatctgc cggcgaccgg aatcagtgcc ctcaagccgg gtgagacgaa gctcaagctt
    31141 ggcaagcagg cgtcaaaggc cgaaggatac atacgcaaag ccgtggtaag agacgacgaa
    31201 gggaacgatc attcgctgga actcgtggcc gcccgacgat gctcgcttga gcaatacatg
    31261 cagttctaca acgtcacgct gatgaacccg aaggtgctgt gcggtctgtt cgcgggggtg
    31321 ctcctggtgt tcctgttctg ttcgatgacc atgaaggccg tcggaagggc cgccaatcga
    31381 atgatgatcg agtgccgtcg tcagttcgag aagatccgcc agttcttgcg caatcagggc
    31441 aaggatgaaa cgtttgtgca gaacccggat cattggccca agcgagtgga agtcgaaggg
    31501 cagcggtatc cggattacgc cacctgcgtg gcgatttcga cggcgggtgc ccagcgggaa
    31561 atgatcgtgc cggccctcat ggcgatcatt attccggtgg cggtcggcct ggtattcggc
    31621 gtgccgggcg tgatgggcct cctagccggc ggtctgacgt gcgggtttgc catggcaatc
    31681 ttcatggcca acgccggtgg gacgtgggac aacgccaaga agctgatcga gacgttcggc
    31741 aagttgaagg cgaaggacgt cctggagaat aaggaactgc aacagaggct gccggccggc
    31801 atcagagacg acctgctgaa caaggccagg caggcagtcg cggccggcaa gccagacgcc
    31861 acgatctacg gcaagggttc ggacgatcac aaggcggccg tcgtgggcga cacggtcggc
    31921 gatccgttca aggacacatc gggtccgagc ctgaacattc tgatcaagct gatgagcatg
    31981 gtgtcggtcg tgttcgccgg gctgatcgtc aagtttgctc cgcagatcgg agcgctgctg
    32041 ggtctgaact gaccagatgc gacactttac ggatggttgt tcatgcgccc ccggatgact
    32101 cgggtcgtcc gggggcgttt gggggacccg gcaccgcgcg caacaacgcg tcagaacgga
    32161 acatcgcaca caggttgagc ccgcttcaat ggacgtcgca gcgcgcgtcg ccggccgtgc
    32221 cggtcggtca cgccggcgag ctctcgcttg cgatcggggc cccgcattca gggcagatgc
    32281 cggacacgtt gcctgtcagg ttgtatccac agtatcggca gtgaggtgcc acccctgtca
    32341 gggcggcttt gatttcatac atgtacttgc ggttcaccgg ataacgtacg atccagacca
    32401 aagccaggag agtgatcagc gagccggcag caaaagcgac gaacatcatt ctccgggcca
    32461 cttccaccgg ctggtgctcg gcattcgagt catagccgat cgcatcgagg caaagaccgc
    32521 acagaagctg gccaaaggag atcgacatct tcaacacgaa gctcagcatt gccgaatagc
    32581 tgccgtcctt caggactccg gtatagtacc tgttgatttc ggacacgtcg gccatcatgg
    32641 aattggcgat ggtgttggcc gaggtggcgc ccaagtggta gcacacatga aaaaccagga
    32701 acacaagcgt tgccacggaa gtggcctggc ctgcaacggt gaacggagtg ctgcccggac
    32761 gcacgagacc ggtgccaaac agcagcacga ggacgatgtt tccgcaacac gccccggcca
    32821 gaaggacaaa gatggccggt tttttgtcca gccgtcgaac gagtggagcc gccaggatgg
    32881 cgccgatggc ggcggcaatc atcgtgctgc cgtgcaccag cgttttctcg acccccgtga
    32941 acttcatgaa gtcgacatag atgaacatct gcaacgaggt aaccagtaca acggcgataa
    33001 acagcacggc gatgaagacg aacaccattc tgggcagcgg atcaagcagg acggcagtca
    33061 gattgcgcag gatatcgcca agcctattgc tgcggacctc agggttgttg cgagagtcga
    33121 cggcgtactt gcgcgtcgca aacaccgtat atagcaggaa aaacagggcg atcgcggaaa
    33181 aggcgaagcc catatgtttg aagttgtcgg gaatcgtgac gccgctgccg atctccccgt
    33241 cgcggtcgcg gaggaacacg gcccaaccca gtatcgcggg gccggtcaga ttgaccgcca
    33301 tgttcacggc gcctcgcacg ctctggagcg tggtccgctg gttatagtcg gtgcagatct
    33361 cgaagcccaa cgcgacgaag ggcacggcaa acaccgtgct tgcggttcgc agcaacacgt
    33421 tcatgacgac gagataccaa aacaggagct gcggcgttcg gaaggtcatg ggcaccgccc
    33481 agatgccgta gaaacacagc accatcgcga aaccgccgat cagcatgtaa ggatgccgcc
    33541 ggccgaatcg cgagcgcgta ttgtcggtaa tgtgccccat cagcgggtcg gtgatcgcgt
    33601 cccacaaggt ggtaatcgcc atggcaacgc ccgcgttggt accgctcatt cccatcgcct
    33661 tggtgtagta cagcatcgcg aagctgtaga acgtgttcat gaccagggag tttgccccgt
    33721 cgcccatcgc gtaggcgagg atatctcgca gtggggtctt ccggacggat actgcggtat
    33781 cagccattgt cttttgctcc cggccggcag cacgcggcgc ggcaatctct acgcttgggg
    33841 acggcttgat cttcgcgtgc gcgctccgcg ctccgccgga atcctaacgg accagccggg
    33901 caaatgaaat gccgggacaa gggcacccct ggttacggcg ccttggtggt ccgggagggc
    33961 ttggcctcga cgggtttatc ggcgggctcc gttaacggga aggtcttggt gaactgattg
    34021 aggatcgaat cggccgaatc gagggccttg cggcatttct cagcgtcgtg ggcccgggcg
    34081 gcggtcctga catcgtgaac cgcatccatc agcttgccgg ccagattgcg gtattgttcc
    34141 gggttgtgcg acaccggcgc atagccaagc aatgctcgaa catcggccat ccagtcctgg
    34201 gcctcgtcct ccaggtcttc ccagtcgcgc ttggtgaact cctcgcttac ccggatgtgc
    34261 tggcggttca cacgttccag ggtggcggcg tacggcgtgt aacgaagtct cggggccggc
    34321 gcgggttcag gctccttgga tgaagattcg tgacgcttgt aggcgttcac tggatccatg
    34381 acgtcgacgg aaacatctgg cgttttcacg gagacacacc ccaccgcgag agccagaata
    34441 ccgaccaatg gcaacatgat ggaacgagac attgcgggac tcctggtacg tgttgaaaca
    34501 ctctgtctat tctaccggtg gagtcggcgt ccggcaccta ctactacttc ggaatccaag
    34561 cggggttggg cccagcaaat cgaagccgag acatgccgga ggaccgtcac cggggtcgca
    34621 ctggaattca agcatagctc tccaaaggtc agggcatacc ggttcggcct gactgccggt
    34681 atagtgccca ctatggcaga cacgggtgaa cagccaacgg tttggacgat aggccggctc
    34741 ttggactgga ccgccaagtg gtttcgggaa aagcaggttg aagggggtcg cttggccgcc
    34801 gagttgctgc ttgcccgggc actcaactgc cgcaagatcg acctctatac gcgatacgac
    34861 agcgagccaa gcgaggatca gcgacgggct tttcgcgaac tggtccgcca agcaggcgat
    34921 catacgccga tcgcctacct gctcggctcc cgggagttct tctcgttgga gtttcaggtc
    34981 ggtccggcgg tgctcatccc gcggcccgag accgaggccc tcgcccagcg gatgatcgat
    35041 ctgtgccgct cgacccgtga gcggcggtgg cggatccttg acctgggcac gggtagcggg
    35101 tgtgtggcgg tggcggtcgc caagtacgcg ccgaatgccg tgctggtggc cagcgatatc
    35161 tccgaggagg ccttggccgt ggcccgggcc aacatcgagc atcatggcct gagcgatcgg
    35221 atccggacgg ttgttgccga tggattcgcc ttgccggccg agtgcatccc cgaaggtggt
    35281 ttcgatgccg tggtgtccaa tcctccgtat atcagcgagg ccgtctacgc gaacctgccg
    35341 ccgaacatcc gcgaccatga gccgagagtc gcccttgtgc ccccatgctc tgacggcttg
    35401 gagatgtatc ggcggttcgc ctcggaagcg ccggcggtgc tgggtcccgg cggcaggctg
    35461 ctggccgaaa tcgggcatga ccagttggcg gctgtgcagg ag
//
LOCUS       DAOTPW010000020        34824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1322_length_34824_cov_5.982858, whole genome shotgun sequence.
ACCESSION   DAOTPW010000020 DAOTPW010000000
VERSION     DAOTPW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 34824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34824
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1322_length_34824_cov_5.982858"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="P5316_03105"
     CDS             <1..525
                     /locus_tag="P5316_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRR83974.1"
                     /translation="GPQASMPGDQDEDELVGTPLPNPITAADMIAICDRFTVETAPVI
                     PGLINVNTASVIVLQTIPGLSPEQAAAIVAQREQISGDQKTSIGWLVANGVLDAETFA
                     LVSNMLTTRSIQFSADVIGFADHTGTFKRFEVVVEMRGHLAQIVYFRDISSLGAGYPV
                     WNDKQGEGLSYDDL"
     gene            512..2080
                     /gene="pilM"
                     /locus_tag="P5316_03110"
     CDS             512..2080
                     /gene="pilM"
                     /locus_tag="P5316_03110"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRR83975.1"
                     /translation="MTIFDRPVLCIDWDERSLRVIEAAFSRSGVRLRKAVHVPLGPGV
                     NARDPASMGDFLNRTLREHRIRAKQVLIDVPRQDVILNLITLPKGTRDELAAMVHVQI
                     AKELPFSKDQAAIDFAVVPGNGGTSCDVWVAAVRAHVIEYYQQTLEAAGLTAERIGLR
                     SSASQAAVIEGGDGAGRTVLVDIGPSMTEICVIRDGRLVYSRAASVTVMAEPAYPEER
                     QHPPEQTIPPADDFLPKQGPLDALLIEVSRTIQAYRASDAGARIDRIILSGSGGVDER
                     VRAAFENRFGSPTRVFDAPPALNWKRIADVSAAPFIAAIGLAYNHLADEMGRFDFLHP
                     KEPEAERKERAKRRPLVALTVALFLAAGVVWAVQTLRLQQKKIDEVVAERQQIERQTK
                     EYADLLKKHEDLAEWMRMNVVWIDKLKLLAEHFVSNEKGYITELEIKDPGQMIISLAS
                     KDNSVGTQLAEQIRKITTSRPAPDGKVTTVSEFTAEVGRVKPNPKDPEYPFIDTVTVR
                     VKSLTTPAKGRRSG"
     gene            2090..3205
                     /locus_tag="P5316_03115"
     CDS             2090..3205
                     /locus_tag="P5316_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83976.1"
                     /translation="MEPREKLLVGIVVAIAVVGAAFGARRLYVDRANKLEVSLKYEQR
                     KLSQAKKKLEDAKAKELEWKAIGRQTLSTDEMVAKGRFRGELTRVATESGFFVPPTQV
                     DLISTVPHPGHSGLLLVVYQVKAEGTIDQIVGFLHRLYREPYLVRCKRILLKPLMEKS
                     SSPNAVPKPTGRLSLTMSVETPILPSDKRVPQIDTAELAPDKRVPVGRTLLASADDYR
                     GIKRELFERYVYQPPPPPPPPPPPTQPVRDPPALPPPPPPPPADAELVLARILSSPRS
                     QQVVLQDPRNPNAEDKRVEVGDSLYGGVLIFIHRDGAVSEKQDKDGTKRTFHLIGQPL
                     KQGQVLSQTEHPAVYDALAKLEERLAGINKPEKAASP"
     gene            3382..14040
                     /locus_tag="P5316_03120"
     CDS             3382..14040
                     /locus_tag="P5316_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRR83977.1"
                     /translation="MTTKTYAKRIGSRLALVPIWAVWFLVSGLAVGQDGNGTEEAGPA
                     LPAATTRPAPPIRRYPNMNEALRARAAASRPAGPHVHEPGTISPSDPDNDGLPTPLDL
                     KIHSEADQLEVLDDAQLEALKAAAKASTSRPSGLPNQPAMPPRVPLRKPPAPGEPAST
                     TGSVPPAEPTVTTEEPQPTLEPTAAEPEGQDVPADEPIIHEELPAPAEEPVPPATPSP
                     GTDAHPAVPPRPVTGAPTTGPAHTPAPPTGRRPGAGPGGRPGSPSPPQPVPPTHQPAV
                     TDANAGMNVPKLDPKTYWKLPYYERPYFFNWSNTPLEKCCQDFVEMSGLSMVGLLSEG
                     VNDADRKKPITFQSLELQTFDQALTRFNLITQEVGMGYWTMVRDGYLEFRQLSEWYRR
                     MPAERLYDSQEAYEKANLPEWEMASMMYTPKHQPAVRLAQWVNDSVPDNCARATLFPD
                     SSWIEIRGCVYHINRALEILAKRDVEIKDGREWRVYPLKHRTPAEASSQLQGMLLPEG
                     LMGGATGAVPANPVPTPAPPTGPPGRPGRGGPVRSPAGPTEIGTSTSDDIEIQEPLSG
                     SSLLVLASPVKHKRIEELLTKYIDVEGSDPRKKEIIELKHVDPSEMIDLITPFLGEPK
                     MVQQPAPPPKPGQPPTPPPPPQIMKVNTSATLTPRPPRSILVEANDKEMREIKELIAL
                     LDVPREESKYQVVKLEHADASSVAMVLTSIITGRQTSQPRYPRPGGMPVSSVGGAEGF
                     KAIPDNTTDRTLVLSGSQQDISDAKDLIKLLDVDPTAGAAEHIVQLKNAEPGSVVQIL
                     TTRFGGSSSGGGYRRGPYGYGGYGGGGGQSLPQFIPDDASKMLVVICHDDMWPDINRL
                     INELDEKSETLKTTRSYQIKYANAESVSMLLSQALAGGSSYGSYRRYPYGGGGGGGGD
                     RQPSFLVNPSTNLLLVTATEEDHVKVAELIEKLDQPSPADQAEFRPIELEQADAEYVA
                     EKIEEVLGGEGGSSRRRSSRSYDYYGFGGSSEGAPKVPVRVIAETIGNRVLVSASDED
                     FKKAEALAREIDAQYIKQEIIRKTFILENIDAWEAQQIISSMFDAQSSGGGRSSRFGG
                     GYGGYGGYSSSGLGSSSSPGSVKIAPMGNGLVVQAPKDKMAEIEKFIAEIDKNPTAGN
                     EVKMYKVKSTGYEGSAGVARTLCTLLDIPDTSSRWGPASNTKSPIKIMGEYGSNVLLV
                     SAPKEKMPEIDKQVQAYIDAQGGDTTMILKHFEVTKARPQEVADIIQPILDTKYREMQ
                     ESSGGRRGYYGSWYSVSSGGPQITVNKITRKLMVMAPAELMPTVEGLIREFDQASQPS
                     VTRTVVLKTAKAEEIAPIVQEHMKASAGPSPGRSRFRSWEGGFGDYSPWGGFRRSSTS
                     SEGGEELVVTAFEAGNSIILRGMEEKVYEAEQFIKMLDAGAQPDGPMIKVFQLEFADP
                     YEVADYIDEVTGGGDSTSLTSTTPRRVSSSSSAISVKTDYATKRLIVAAPPERFPLIE
                     QVIEIQERLAREAAARPVLEGDATTVVGAGGTIKKFYTVEGPAKEIAKRLDEIMYGMY
                     GYKGPWVKYFPYTNQVMIDGDPKYFKSAEEWLAKLEKNPPPVALKVLVRRSTVAPSKV
                     LPHLERIVTTSLREGAGIRKLEAGGIGRDPMSMLEEIYYDTPIEEPKPTTRPAEEQSS
                     SARSGIDVVSQFVPTGVLARIERDLAAIPLAQISVISRNAATKPAVAPATTRPAAAPK
                     VGTHPSQVTPQPPVPPAGNPQVKEAKTAVPKGAPGPRGTSEAAPVNRVSGQQAGLGDS
                     AKAASRDEMTAEERQTAELFQAAAEYRMSNKPQILYDEVQGTFIIFGSEDEVEEIDEI
                     LDMIIKEIGEIEEGAKGDIRVFRVKHVDVQVAASMLEAMFNEPAKAQPAPRAPRAQER
                     KPQAKPGGAKSGEQEEEESAAQRRREEEEQAKAKEAEEAAKAAAAGNIKVVPDPRTNT
                     LIIRAAQETFPKIAELLLKIDRPGLDKEKNIKIFQLKKLNAAEVEEVLKEILKIETPQ
                     QRRTFRPRLPGRGGAGVDVDMIEALEQQMLEFQQSMIESSAPVAEGEGKEGKTKINPA
                     KEISIASNATTNTIIVTAPEEGMKLVETLINRLEEQDIPIAIETVELQHAEAEKVATQ
                     LQGVFETAQRRGGGPRGAMEGYTPSRMGEFKVAADSRTNRLVIRALQTDMERILKIVE
                     ELDSTPSNPVEIYPVEKASAEDMAKTLSEIYGTPGGVGGAQMVRITADRSTNSILVRA
                     PKAQQMLIAAKIKDLEQQSVIQGEVRQIQLKVATPSSVASQLTQIFSPKGAARGTRQQ
                     INIVGEDASKMLFVTCPPDMFEEVKKIAEQMDQITEQQVKVTRLTHAAASEILPKLNE
                     MAARLIAQTGLKGTGDAVFAATADERTNSLILAGTPKALMVVDAVIKELDVTGSDTTQ
                     PTTKMFPLNRGNAASVAATINQLYGSKRWQAGVEAPKAAPDPIANVVFVTGTTAQLQL
                     IDDQIIKPLEGYSPARPVEVAVNEYEVTVNYADVEELAARLTTMFNQGIQARKSAGDT
                     SLTALDTAMSIVPEPSTRKLFISCSAESKKKIDNWLKGLDVEAASFEGQQTKVLPVRV
                     ADLTYVSNALNAAFKKSGKVPASEQVTILPEYGTNTIVVKALKKDIDKIEQLLHEIDK
                     EDRERMVAPEAIPVKHILPTQLANRLTAMIRSSYAADRRTNQYPVSVEPNDTAGTLLV
                     TAKNQQAMDSIKELIAKLDSQPAEDLRLTKPYVLKFADLGSTQMMITKRFGKQDQLPV
                     RDQVVVDVDYSTGALVVTASEENHAKVREIVEDLDNAIVDDPKMPETIKVKNVRASLL
                     AKTLTDMITQTMKPNKLTRQYPVTVQANDTANTLVMTANKADMERFKKIIQELDVAPD
                     TEDTRVIKPYMLKFSDPSVVTKAITDQFAGQEQRPLQEQVTAGPDYATNSVFVTASEE
                     NHKKVQAIIDALDQSDAAGRKTYTLDLKNADPEDVAQTLTTVYNSGPRTRTGRTPAVF
                     TPVPGARKLLVTCTPGELEQAQAMIEQLDISPQEGGQKRAMRVVQVKKLPAREMMTML
                     TEYLRKPGKSGGRDPSLQDDIKITASDAADAVVLTGPEERLEELQQLIEKVDSAVVDP
                     SDPNAGGRQYAVIPLTNADPSSVATVISQTYPARGNVPDSERVNAVAERATNSVVISA
                     SAEKLQIIRKMIDELDQQSTNVPQQEIIRLKNARAEDILEVLRQTYQMGRRGMSSSGP
                     PITFAADSNANALVVSAGKADMEGIKTMVAELDKPVTDEVDQLRIIPLQFVDAQETLE
                     ILNEYLRKPGATAGRRGSTSELIGDIRLQASATLNALIASGSQDELDRVEKIALDMDK
                     QVEGAGAPKIIPVKNAAAATLAGTLRELFANPTQSRSGRRTTQNDVPVIMSDDSTNSL
                     IVRARTVDLNLIEDMVRELDKQTEGPSGLEVIPVSRGVDVVSLARQIQDVINAGERAK
                     ASSQSTYKPRQVAIAGEERSSTLIVSGAPELFETVRKLATELEQKRPATGGRRALVVP
                     VKTKSAQDIQKVLEAYIEQQNGRTQQRR"
     assembly_gap    14267..14366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14395..14494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14495..14692
                     /locus_tag="P5316_03125"
     CDS             <14495..14692
                     /locus_tag="P5316_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83978.1"
                     /translation="GFWGQREGILGPARRDSGASEKGFWGQREGILGSARRDSGASVG
                     ATGGLPASVDLAVREPLESGG"
     gene            14832..20093
                     /locus_tag="P5316_03130"
     CDS             14832..20093
                     /locus_tag="P5316_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRR83979.1"
                     /translation="MWYSWLILIAAVGQTAPPESFGPIAKRTIISGDRVTVIPVGEVT
                     DSPTTTRPAAGALRRVGRIPTASRPAASDGSRAGDESVLSPRPARAVPLTVVTKPTTL
                     PSEATASSTPLPLRGPASRPESSVDLDALRRKLQASSAAPTVAARAPAAPAPSAVEIR
                     ELGPLSGDTVSIHSTATGELIIEANEEDLKVIEALINLLDAQPPEAEQTVRVFTLQSA
                     QATDVATNIQKLWNEYKKPVSGQIRPEDRLTIIPDPRANLLMVATAIGNLKEVEMMVE
                     ALDQSPIGGNVKVVPLPLKHIKAAEAEEAIKNLLKLLQQQRGASKELYTLYADVRTNM
                     LLVSASERDIEQIRQLLDIIDVEPSPETGGVAKVAIVPLKKAVAADLVKALNDMLVSQ
                     SDAAKAMKEQIRRLQVVLAKDENQKELKPVDLEKPIKLFAEPGTNSVIIATVEGNIEP
                     LKEIISELDGMPLADEIMLSLFPLEHADAVSLRDDLQKIFDQSQKVTDVPGKEAVKGR
                     TPDTITKGLMAPISLVADKRTNTLIVSGRPEQLLLVQQIVKTVDVDKIANKFPVRMVK
                     LEHSDVRRIREVAQQLADQRQKIAERLGEMAKERESILLIEDIRTNSLIIAANDANFQ
                     EIEDLVKKLDGTEDDWLGQIKIINLPDNLTAPDIADKIGELWQRRAKLRQEGGLPADE
                     PVIVSDSRTNSLVIASNMEDFEAIQKLVDQLGQQKLSPMLDIYQVRVQNNDAGKVAET
                     VQDVMDKRLELSTDPNAKEQPSERVYVVADPLTRMLLVVASKRNYEEVVSLVNKLDTP
                     PPVDGIIRVYYVRNIDVAKAEKTITDLFEKGIYKAGVDDKNLPDSMKKVTVIPDVRSS
                     CLIVSASPENYAIVEKVLQEIDRSETPMAQAYARFFDLQHADAVKAAETLKTMLEGLK
                     KSMPGDQADELGFEITPEVRNNRLIVVGTRYAMQRVEEIVPKLDIAPRIPSGITQFYE
                     LKEASASQVAKSLTDLFEKQTPKGEQTPITIIPFDASNGLLVTASQDDHIKMQELVGL
                     LDRKTKISDQMEIISLSEAKAEDVVKTLTDLMEQQQRDREGGYTVAAEPRTNSVAVWA
                     TPDLMVQIKEILRKIDLAGPIDTRSMRVFKLHSAKAEDLAKLLDDFFKNAGAGGSDGK
                     NVRKMIIDFVAHRNPDSGEEITQKLVHQDVTIVPDPYTNSLLVLAPAGSIDMMRMLVE
                     MLDSIEPQTISLRVFELLNADAEEMRDLLEGLFKTQGGGSSGSAPQITLGEGAAAGLA
                     AAAGVSGTGGVLEVEFAVDKRTNTLIAAGSPAHLRIVESLVLQLDYRELDERIAKVVQ
                     LRNTRASEVAKTLTEYFDQESELMEKAFGEEASQRRFQRQVIITEAATGQIAADESGG
                     SSNKKESNLLLVSCSPRMESLVYRMINELDQPIPQVMIQMLMAEVTVDNSFDLGMEFA
                     AQDLLFSETATLNANTGLPQGGDYDIVFGTDLGVAAGGGFSFTVTGEDFSFLLRALQG
                     EGRTEILARPSIMVQDGQAAFISIGDQVPIVKSASVSQGVVIPTVDYVDATVKLTVTP
                     IINPDNFVNMYIKPEIKNVSASSQDLGNGIKAPIINTREAETSVTIKDGETIIIGGLI
                     KGKRDDTESKVPIVGDIPILGSLFRSTTKRDTKTELLIILTPRVMRAVEDARKLSVEM
                     RDQTGIIDDRIRTNPLMQGLQVKPTDVEGLQTPAQTRPADHEAGEDELFGPEIDEYGP
                     AASSVRTGPVPAVAIQPPVTVSGKARR"
     gene            20212..20793
                     /locus_tag="P5316_03135"
     CDS             20212..20793
                     /locus_tag="P5316_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83980.1"
                     /translation="MAMILTNSGIHFRRVGHLFIAAAGTLLATGCVQPQEAATGSGGA
                     LVQEVARMPTEPSVVSIVAFYKSTAPWLWNADRTRVSGIYVSALYLLGPKGLGVFGDG
                     IIRPRMFVVETGKDGKRQSKLVKEWAFNVEEAVPFRAKEKKTLGWGYGLPLPFGDLDV
                     SGREIQMVICFERSDGRLVTSSKQTFLVPKSTE"
     gene            21065..21574
                     /locus_tag="P5316_03140"
     CDS             21065..21574
                     /locus_tag="P5316_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRR83981.1"
                     /translation="MRKIYPSGGPVPAQLDEAILAAARVRIATRRRAWIVLRRVSIAV
                     TAAAAAIGLAVWIGQGGLKSTPSPEMMIAAVTPAGPKDIDRNGRVDILDAFILARHLD
                     ERLGTTADTSGADALVGPQDSAASARKASGRGEARPSDWDVNGDGVIDQGDVDAVAMA
                     AVSLKGGAS"
     gene            21772..22239
                     /locus_tag="P5316_03145"
     CDS             21772..22239
                     /locus_tag="P5316_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83982.1"
                     /translation="MLVCVAALASSVAFSVVVARAQEVPATQSASKPAIRFTHVDVFI
                     DAKDQPLAAYQFELAAEKGEVKIVGIEGGEHVAFKAPPYYDPAAMSRDHVIIAAFNTG
                     KDLPKGKTRVARVHVQVTGETKPEYVIKLEVAASSEGRPISGATATVIEGGSR"
     gene            22509..24542
                     /locus_tag="P5316_03150"
     CDS             22509..24542
                     /locus_tag="P5316_03150"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRR83983.1"
                     /translation="MRFRGKWWGQDAAKPRDAYGIGGRRWSLLREGKSPPPGALPSPG
                     EELWIVQRPSGEQPRPTGEDIPGSGELRAKLPGETKEIPLPLKHTDVQAAIAGYIATV
                     DVTQQYHNPYESKIEAVYVFPLPENAAVNEFLMTVGERKIRGIIREREEAEKIYQEAK
                     SQGYVASLLTQERPNIFTQSVANIEPGKQIDINIRYFNTLAYDDGWYEFVFPMVVGPR
                     FNPPGSTDGVGAVGRGDRGISGQKTEVQYLKPGERSGHDISLSVAIDAGVAIEKIDSR
                     NHVIEVKKSSPEKAEVRLGRLDTVPNKDFVLRYQVAGQTVKSAIMTHRDKRGGFFTMM
                     IYPPKDLSNLKRKPMEMIFVLDCSGSMSGRPIEQAKAAAERALRLLEPDDTFQIIRFS
                     NNASQLGPKPVVATPDNVRRGLEYLKSLQGEGGTMMIEGIKAALDFPHDENRLRFVSF
                     LTDGYIGNEEQIFGEIHKRLGAARIFSFGVGSSVNRYLLDGMARMGKGAVAYLGLNDS
                     GADVMNRFFERISHPAMTDVMIDWGGMKVEDIYPKRIPDLFVGRPVLITGRFSGKGSA
                     TVRVKGQAGDKAREIAIPVDLDDASATHEGLAAVWARMKIAELSQHAIMDPGEQWTGQ
                     IKTVALEYGLMSAYTAFVAVDSLTRTAGDSGTTVAVPVPIPDGVKYETTVQER"
     gene            24683..25351
                     /locus_tag="P5316_03155"
     CDS             24683..25351
                     /locus_tag="P5316_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83984.1"
                     /translation="MRWCRGTAGVLICLINVGCAIPIPYLFPHVSRTPMIASPVLADD
                     VKAFRVEYTYHQHGCVTLERQPADRVEMLQAQGSPPAVPSQSRLSLAVGIYLFNPALP
                     HNFGSDGCVDVRLYRRSFETISVHGHSTNEGLRSARWTPVSDYQAREIAIDMVFVLAP
                     GRLLPVDSCPPDLVRLKCSTEAARFAADEYDAVANEPGIAAKDRERITRKAEYLRGLA
                     TKGC"
     gene            25817..28189
                     /locus_tag="P5316_03160"
     CDS             25817..28189
                     /locus_tag="P5316_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83985.1"
                     /translation="MSTRTDSTDREDSRSAAVAVDSALPENGPEKARPAVSDEESENA
                     PASPAEEVAAVQPSTGTPAQPDSHNTPEASNHEERPPHVDDEPVGLAAVEAELVAEVV
                     EEVCEEIAEPSDTGWQGELPDLLMRIVDARISVWQKLLEMDARRAEMEKLARAREIRD
                     ELSRQNREIKNNPNAEVLQRALERQQQLLRNPVIKSQVPPPDPKAKTPDVDQNQLYRN
                     AIQMGLGQIALLLHRRKLDDSVPTAAVTAIADEPLVLLCRNHGLNADPLMGWTYYALG
                     VQKRIDEFVAAEKEHRAKLAEARAEDKENNRGLGGLFRGGSKENQVGELSPTVRAGLQ
                     AASREMQAIEPQLTEMFWRLYEDLAWLLVQGGLDEKEMVVARSFLRYGLISVHPGLLQ
                     PEKVDFIIKDCQEDVYEWQNRPQSTHLVYADEYIEAVASKNLTVSPDENLELNERGSD
                     SWKADRVWRQAVICRVRSDLFNARLAELKALIERKQAEIEKKLEEAKVLRETPKKVQQ
                     AKELEKQVMMIKPAIARLRHATEHMEARIIPKVQDQADEATERLGEAAKVFTEEMLVR
                     REARFIRYMARLVGRLKEPFAQFVLRDHFKPNGPDHNSRRVVLEAIKKLESEDRRLFH
                     HVLMPNKRLDRQITVRMSPTFLLPPGRGQMGLAVSPRKWDDNGRIVLPLLAAKAGDLE
                     KLLVNVMADFRWDCSKEDAGMDWIVADALCAAYAAARWNVRKLPEKAQKMMGFDFKLK
                     ERGNFRIHYALYVNSAHQGGRLLFSRSDEVYKVMVKYIGLPPGVQMLKRD"
     gene            28500..29054
                     /locus_tag="P5316_03165"
     CDS             28500..29054
                     /locus_tag="P5316_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83986.1"
                     /translation="MRRSVLMIVGLAGLALLSEGCMRAASEGMEAVRGGQGAYFEENL
                     LSATPKDHLALSGYQRIELGEFTNQSGQAMPPEFPTLLAAKFNERLAKSDLPKETSGK
                     TLLFKVTLIYYEKADLKDSVFGPLEEAVARAELVDKDTGKVLASGAVVGRSSQSVGLG
                     PAFKADGMAKALIKWASDYYPKPK"
     gene            complement(29223..32564)
                     /locus_tag="P5316_03170"
     CDS             complement(29223..32564)
                     /locus_tag="P5316_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR83987.1"
                     /translation="MKHPSRFAVIGQAIAVGLVFFLRSAPVLSATAERTTSEETPSAE
                     KANTTGGTLHGGSAGWLTNLNEGYAEARRSNRPIVVRVGATWCGWCRKLNDELKDSSV
                     QESLSSYVRVYLDVDDSPRDARSLGINAVPALRVLNVAGRVVASHDGYMASADLAQWL
                     SGQLPVATAVTPPGLRKDGPLDAKTLHELIDQLRGREALVKEAAIQRLAAEPKLAAPA
                     VVTLFAEGALSERLAALELLDGWRAPIRDLDPWRPETITLQRLEALRAWARRMSDAPA
                     SMPAQLPDDVLENARRELIRLVQADRDAEAEIVRERLARLGPSLLPVVAGELQQAATD
                     RDRERLTALRYRLAAPTRLALEWPGGFDRLASTDAPTRQQAAEELASRAQADASALLR
                     ELFSNPDPLVREISLRTLRKVGGEEANEALMSLLGDPEPNVRAAVLKQLTEQPSPKLI
                     PRLAEYAAAEQDADLLVHAVRALRAAGGKDALEALSRMTGHDAWQVRAEVAEAIGEVL
                     IESGNLPRQSRADAYVTLLALLKDPDGFVTSRAMTSLDHADVSLVTEPLVQAALDHPE
                     MAGSLIEMLTRRGGDAEQTGTYLRKLLSAKHASIRAAAIEHFCRVLPRQSEKELLTAL
                     GDPEEEVRFAAAKGLILVLTSCRPSETPFTSYEYRTEEIAGTGSSLIARLLGLSPRRV
                     VVRVASSSPASAPSQSQPVADADWLANFRSGIDRPKWMDQAVAPLEGLLSSSQWEHRL
                     TACIGLIALGRDETALAVLRDVVRSQNAAVSQAAQALGWLPLGERVSLFRELLGLAAE
                     TQLMVDVINGLAILPDPQAAAPLWELLGQKDFALGWADTAQASLLRLYLGPEYEWSDV
                     NAERRRRLVTEATPMTEKGPEVRQLVALSLLLTGSKSDAVTAARRLYEAPESNSPLRA
                     DALQIMLLGQSEAQAVETAATALASPDDHVRGTALLYLARGKTQLDKLRGVLSLHIFS
                     EAEAAFLDFASSGGQPISPQAPQGLTVDNVRPLLRSGDATTRAHAGYLLALLKQPEGL
                     EPLVEHWRENARQDSTWRRQVYRAIAALNDDEHTPILEEIYKDLAGETSAMREFYWTV
                     RSMDGEKVFALRKKIRTEVGMDQLR"
     gene            complement(32561..33970)
                     /locus_tag="P5316_03175"
     CDS             complement(32561..33970)
                     /locus_tag="P5316_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRR83988.1"
                     /translation="MLLLADKPLRSGCLVIGVCAVVLASCLWSCREEKSPPANERRVV
                     VVVSGDTAGWIIPCGCTTHQSGGLLRRGTYVEKQREEADVILLDAGGAPGGTSPYQRL
                     KFEAILEGERLMGLAAHNIGGPEVALGLANLQEASRKTGARLISANLRDSVGRPVFDG
                     WTIIETAGRRLAVIGVISPRYAAGDVRIEDPKAAVLASLQQLEGRHDGVVVMAYLPED
                     ELQRLATELPEVDAVIGGPTGQPIAPRAVGPTVLAAATNKGKFLVQLEFAAAGNAGPP
                     TGQVVELTGDWPDDRGQLANLRRYLEELNRRSFEPHQTDLVPSAPPDVPPGYRVAGSA
                     TCRECHERDNELWDASRHAKAWEDLKAMERHVDPYCQQCHTTGYGLPGGFVTVAKSPD
                     RLGVGCEACHGPSQVHVEEPERRTPFDAKDQCTRCHDQENSPAFGYERYWEKIKHGET
                     KGTQPDRLGPRPSGTETQP"
     gene            complement(33985..34776)
                     /locus_tag="P5316_03180"
     CDS             complement(33985..34776)
                     /locus_tag="P5316_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRR83989.1"
                     /translation="MTRRSVFSLGKGLLPQGFLIAAVCLAALAGFAQYLSAASGGATA
                     SRPASQPADGQQPRVVPDPKQVGGEAESIVQVANLVYAGTKSSKCFSDHFLVQAEKSS
                     AISTSRRFHAVKLASGELFSFPLVIMTGEGDFVLPDAERENLRKFVNRGGFLLASAGC
                     SSREWDRSFRNEMAKVFPDTPLQIIDMEHPVFHMVADIKSLNAKHGKPRPLEGIHVGG
                     RLAILYSQDGLNDTEHTQGCCCCGGNEITNAVDVNVNVLAYALTH"
BASE COUNT         7157 a        10541 c        11046 g         5880 t
ORIGIN      
        1 gggcctcagg cttcgatgcc cggtgaccag gatgaagatg agctggtcgg cactccgcta
       61 cccaacccca tcaccgccgc ggacatgatt gcgatttgcg atcgtttcac ggtggagacg
      121 gcgccggtca ttccgggcct aatcaacgtc aacacggcct cggtgatcgt gctacagacg
      181 attccggggc tttcacctga gcaggccgcc gccatcgttg ctcagcggga gcagatcagc
      241 ggcgatcaga agacgagcat cggctggctg gttgccaacg gggtgttgga tgccgagaca
      301 tttgcgctcg tttccaatat gctgaccacg cgatcgatcc agttcagcgc ggatgtgatc
      361 ggcttcgcgg accacaccgg gacgttcaag cggttcgagg tggtggtcga gatgcgcggg
      421 cacctggccc agatcgtgta tttcagggac atcagttcgc tgggggccgg ctacccggta
      481 tggaacgaca agcaaggcga aggattgtct tatgacgatc tttgatcgac cggtgttatg
      541 tatcgattgg gacgaacgct cgcttcgggt gatcgaagcg gcgttttcgc ggtcgggcgt
      601 gcgcttgcgc aaggcggtcc acgtgccgtt ggggcccggc gtcaatgccc gggatcccgc
      661 ttcgatgggc gatttcctga atcgaaccct gcgtgagcat cgtattcggg ccaagcaggt
      721 tctgatcgac gtgccccgcc aggacgtgat tctcaacctg attaccctgc cgaagggcac
      781 ccgcgatgaa ctggcggcca tggttcacgt ccagatcgcc aaggaactgc ccttcagcaa
      841 ggatcaggcg gcgatcgatt tcgcggtcgt tccgggcaac ggcggtacga gctgtgatgt
      901 gtgggtcgcg gcagttcgag cccacgtgat cgagtactac cagcagacgc tcgaagccgc
      961 gggcctgacc gccgagcgca tcggtcttcg ctcctccgcc agccaggctg cggtcatcga
     1021 gggcggtgac ggcgcgggcc ggacggtgct ggtggacatc ggcccatcga tgaccgagat
     1081 ctgcgtgata cgcgatggcc gtctggttta ctcgcgagcc gcgtccgtga cggtcatggc
     1141 agagcccgct tacccggagg aacggcagca tccgccggag caaaccattc cgccggccga
     1201 cgactttctg ccgaagcagg gcccgctgga cgcgctgctg atcgaggtca gtcgcaccat
     1261 ccaggcctac cgggcaagcg acgcgggcgc gagaatcgac cgcattatcc tgtcgggtag
     1321 cggcggggtc gacgagcgcg tcagggcggc gtttgagaat cggttcggca gccccaccag
     1381 ggtgttcgac gctccgccgg cgctgaactg gaaacggatc gccgacgtgt cggcagctcc
     1441 gttcatcgcg gcgatcggcc tggcgtacaa ccacctggcc gatgaaatgg ggcggttcga
     1501 cttcctgcat cccaaggaac ccgaggccga gcgaaaggaa cgagcgaagc gacgaccgct
     1561 ggtggctttg acggttgcgc tgtttctcgc ggcgggtgtg gtgtgggcgg ttcagacgct
     1621 ccgactccag cagaagaaga tcgatgaagt cgttgcggag cggcagcaga tcgaacgaca
     1681 gaccaaggaa tacgccgatc tgctgaagaa gcacgaagac cttgccgagt ggatgcggat
     1741 gaatgtcgtc tggattgaca aactcaagct tctggccgag catttcgtct ccaacgagaa
     1801 gggatacatc accgagctgg agatcaagga tcccggccag atgatcatct cgttggcgtc
     1861 gaaggataac tcggtgggga cacaactggc ggaacagatc cgcaagatca cgaccagccg
     1921 accggcccct gacggaaagg ttacgacggt aagtgagttc acggccgagg tgggcagggt
     1981 aaagcccaac cccaaggatc cggaataccc gttcatcgac accgtgacgg tgcgggtgaa
     2041 atcgctcacg accccggcaa aaggtcggag atccggatga gataatcgta tggagcctcg
     2101 cgagaagctg cttgttggaa ttgttgtggc cattgcggtc gttggagccg cctttggcgc
     2161 ccggcgtctg tacgttgatc gggcgaacaa gttggaggta agcctcaagt acgagcagag
     2221 gaagctgagc caggcgaaga agaagcttga agatgcgaag gccaaagagt tggagtggaa
     2281 ggcaatcggc cgacagacgc tttcgaccga cgaaatggtg gccaaggggc ggtttcgcgg
     2341 ggagttgacg cgggtggcga ccgagtcggg cttttttgtc ccgccgacac aggttgactt
     2401 gatcagtacc gtgcctcacc cgggccacag cgggctgttg ctcgtagtct atcaggtcaa
     2461 ggcggagggg acgatcgacc agatcgtcgg ttttctgcat cgtttgtacc gcgagccata
     2521 cctggtgcgc tgcaaaagga tcttgcttaa gccgctaatg gagaaatcct cctcgccgaa
     2581 cgcggtgccg aaacccacag ggcggttgag cctgacgatg tcggtagaga cgccgattct
     2641 tccgagcgac aagcgggtac ctcagatcga tacggcggaa ctggctccgg acaagcgggt
     2701 gccggtgggt cgcaccctgt tggcgagcgc ggacgactat cgtggcatta agagagagtt
     2761 gtttgagcgt tacgtttacc aaccaccccc gcctccgccg ccaccgcctc ccccgaccca
     2821 accagtaagg gacccccccg ccctcccgcc tccgccgcca ccccctccgg ccgatgccga
     2881 gctggtcctg gcccggatac tgagttcgcc ccggagccaa caggtggtat tgcaggatcc
     2941 gagaaacccc aatgcggagg acaagcgggt ggaggtcggc gattcgcttt atgggggggt
     3001 tctgatcttc attcaccgtg atggcgcggt ttcggagaag caagacaagg atggcacgaa
     3061 acggacgttc cacctgatag gccagcccct caagcagggt caggttctaa gtcagacgga
     3121 gcacccggcg gtgtatgacg ccttggcgaa acttgaagag aggttggcgg gtataaacaa
     3181 accggagaag gcagccagtc cttaagggcg ggtgcttcat ttctccgggg tgccagacag
     3241 ggtccgagtc ggcattgtcc aacaggccgg gtcggttggt cacaccaagg cgcgattcgg
     3301 gtcataaatg tctgtttcgc agtgctttag acgctttcgt cgttcgctgg aagcgagcga
     3361 acctccagga ggtttcggat catgacaacg aaaacctatg ccaagcggat cggcagtcgg
     3421 ctggcgcttg tgccgatttg ggcggtctgg ttcttggtga gcggcttggc tgtcggtcag
     3481 gatggcaacg gtactgagga ggccggtcca gccttgcccg ctgcgaccac gcgcccagcg
     3541 ccgccgatca ggcgctaccc caacatgaac gaagcgctga gggcccgagc ggccgcaagt
     3601 cggcctgccg gtccccacgt tcatgagccg gggacgatca gcccgtcgga cccggacaat
     3661 gacggattgc cgacgccgtt ggatctcaag atccactccg aagctgatca gctcgaagtg
     3721 ttggacgacg ctcaactcga ggccctgaag gctgctgcaa aggcatcgac ttctcggccc
     3781 agcggtcttc ccaatcagcc ggcgatgcct ccgcgagtac ctttgcgcaa gccaccggca
     3841 cccggcgagc cggcctcgac cactggtagc gtgccgccgg cggaacctac ggtaaccacg
     3901 gaggagcccc agccgacact cgaaccgaca gccgcggagc ccgaaggcca ggatgtgccg
     3961 gcggacgaac cgatcattca tgaggagcta cctgcgccgg ccgaggagcc tgtgccacct
     4021 gcgactccgt cgcccggaac cgacgcgcat cctgccgtgc ccccgaggcc agtgaccggg
     4081 gcgccgacca ccggtccggc tcacactccg gcaccgccga ctggccgaag accgggtgcc
     4141 gggcctgggg gcagaccggg tagtccgtcg ccgccccagc ccgtgcctcc gacacaccaa
     4201 ccggcggtca ccgatgcgaa cgctggtatg aacgttccga aactggaccc caagacgtac
     4261 tggaagctgc cgtactacga gcgcccgtac ttcttcaact ggagcaacac ccctctggaa
     4321 aagtgctgcc aggatttcgt cgaaatgagc ggcttgagca tggtggggct gctcagcgag
     4381 ggcgtgaacg acgcagaccg caagaagccc atcactttcc agagcctgga gcttcagact
     4441 ttcgatcagg ccctgacccg gttcaatctc atcacccagg aagtcggaat gggctactgg
     4501 acgatggtgc gggacggcta cctcgagttc cgtcagctca gcgagtggta ccggcgcatg
     4561 ccagccgagc ggttgtacga ttcgcaagag gcgtacgaga aagccaatct tccggagtgg
     4621 gagatggcct cgatgatgta cacgcccaag caccagccgg cggtgcgtct ggcccagtgg
     4681 gtcaacgaca gcgtgccgga caactgtgct cgtgccacgt tgtttcccga cagcagttgg
     4741 atcgagattc gcggctgcgt ctaccacatc aatcgcgccc tggagattct tgccaagcgg
     4801 gacgtggaga tcaaagatgg acgcgagtgg cgggtctacc cgctcaaaca ccgcactcct
     4861 gccgaggcat cgagtcagtt gcagggaatg ctgctgcctg agggtctgat gggcggagca
     4921 accggtgccg tgcctgccaa ccctgtgccc acgcctgcgc cccccaccgg gccgccgggc
     4981 agacccggtc gcggagggcc ggtacgctct cccgccggac cgacggaaat cgggacctcc
     5041 accagcgatg acatcgagat tcaggagccg ctcagcggca gcagtctgct ggtgctggca
     5101 tcaccggtca agcacaagcg catcgaggag ctgctgacga agtacatcga cgtcgaaggc
     5161 agcgacccgc ggaagaaaga gatcatcgaa cttaaacacg tcgacccgag cgagatgatc
     5221 gacctcatca ccccgttcct gggcgagccg aagatggtgc agcagccggc gccgccgccc
     5281 aagccgggtc agccgccgac tccgccgcca ccaccgcaga tcatgaaagt caacacctcg
     5341 gcgacgctga cgccgcgtcc tccgcgcagc attctggttg aagccaacga caaggagatg
     5401 agagagatca aagagttgat cgctctgctc gatgtacctc gcgaggagtc caaataccag
     5461 gtggtcaagc tcgagcacgc cgatgcctcg tcggttgcga tggtgttgac gagcatcatt
     5521 accgggcggc agacgtcgca gccgagatat ccgcgcccag gaggcatgcc ggtcagcagt
     5581 gttggcgggg cggagggatt caaggcgatt cccgacaaca cgacagaccg cacgctggtg
     5641 ctgagcggga gccagcagga tatttctgat gcgaaggatc tgatcaagct gctcgacgtc
     5701 gacccgaccg cgggggcggc cgagcacatc gttcagctca agaacgccga gccgggttcg
     5761 gtggtgcaga ttctgaccac gcgattcggc gggagcagtt cgggcggcgg gtaccgacgc
     5821 ggcccgtacg gctacggcgg gtacggcggc ggaggcggtc agtccctgcc gcagttcatt
     5881 cccgacgacg cgtccaagat gctcgtggtc atctgccacg acgacatgtg gccggacatc
     5941 aacagactga tcaacgagct ggacgagaag agcgagaccc tcaagaccac gcggtcctac
     6001 cagatcaagt atgccaatgc cgagtcggtt tcgatgttgc tgagccaggc cttggccggc
     6061 ggaagcagct atggcagtta ccggcggtat ccatatggtg gcggcggcgg gggcggagga
     6121 gaccgtcagc ccagcttcct ggtcaacccg agcaccaacc tgctgctggt gaccgcgacc
     6181 gaggaagacc atgtcaaggt ggcggagctg atcgagaagc tcgaccagcc cagcccggcc
     6241 gatcaggccg agttccggcc gatcgaactg gaacaggccg acgccgagta cgtcgccgag
     6301 aagatcgagg aagtgctcgg cggcgagggc gggtcatccc ggcgtcgaag ctccagaagc
     6361 tatgactact acggttttgg cggcagcagc gaaggtgcgc ccaaggtgcc agtgcgggtc
     6421 attgccgaga cgatcggcaa tcgcgtgctg gtctcggcct ccgatgagga cttcaagaag
     6481 gccgaggccc tggccaggga gatcgatgct cagtacatca agcaggagat catccgcaag
     6541 accttcattc tcgagaatat cgacgcctgg gaggcccaac agatcatcag ttcgatgttc
     6601 gatgcccaat cgtccggggg gggccgcagc agccgtttcg gcggtggtta cggcggctac
     6661 ggcggctatt ccagcagcgg tctgggttcc tcctcatcgc ccggcagtgt gaagatcgcc
     6721 ccgatgggca atggcctggt tgtgcaggcc cccaaggaca agatggccga gatcgagaag
     6781 ttcatcgcgg agatcgataa gaacccgacg gccggcaacg aagtcaagat gtacaaggtc
     6841 aagagcaccg gctacgaagg atcggccggg gtcgctcgca ccctttgtac gctgctcgat
     6901 atacctgata cgagcagccg ctggggcccc gcgtccaaca ccaaatcgcc gatcaagatc
     6961 atgggcgagt acgggagcaa cgtgttgctc gtgtcggcgc ccaaggagaa gatgccggag
     7021 atcgacaagc aggttcaggc gtacatcgac gcccagggcg gcgacaccac catgatcctc
     7081 aagcacttcg aggtgactaa ggcccgcccg caggaagtcg ccgatatcat ccagccgatc
     7141 ctggatacca agtatcgcga gatgcaggaa tcgagcggcg gtcgcagagg gtactatggt
     7201 agctggtaca gcgtcagcag cggcgggccg cagatcacgg tcaacaagat cacccgcaag
     7261 ctgatggtga tggcccctgc cgagttgatg cccacggtcg agggattgat ccgggagttt
     7321 gatcaggcca gccagccttc ggtgacgcgg acggtggtac ttaagaccgc caaggccgag
     7381 gaaatcgccc ccattgtgca ggagcatatg aaggcatcgg cgggcccaag tcccggccgg
     7441 agccgtttcc gctcctggga aggaggcttc ggcgactaca gcccgtgggg cggcttcagg
     7501 agatcgagta ccagctcgga aggcggcgag gagctggtgg tcacggcctt tgaagcgggc
     7561 aactcgatca tcctgcgcgg catggaggag aaggtctacg aggccgagca gttcatcaag
     7621 atgctcgacg ccggcgccca accggacggc ccgatgatca aggtctttca actcgagttt
     7681 gccgacccgt acgaggtcgc ggattacatc gacgaggtga ccggcggcgg agattcgacg
     7741 agtcttacca gtaccacgcc cagaagggtt tcgagcagca gttcggcgat cagcgtcaag
     7801 accgactacg ccacgaaacg gctgatcgtg gctgcgccgc cggaaagatt tccgctcatc
     7861 gagcaggtta tcgagatcca ggaacggctg gcccgagaag cggccgccag gccggttctc
     7921 gagggcgatg caacgacggt ggtaggcgcc ggcggcacca tcaagaagtt ttacacagtt
     7981 gaaggtccgg ccaaggagat cgccaagcgg ctggatgaga tcatgtatgg gatgtacggc
     8041 tacaagggtc cgtgggtcaa gtacttcccc tacacaaacc aggtgatgat cgacggcgat
     8101 cccaagtact tcaagtcggc cgaggaatgg ctggcgaagc tggaaaagaa cccaccgccg
     8161 gtcgcgctga aagtgctggt tcgccgcagc acggtggctc cgagcaaggt gttgccgcat
     8221 ctggagcgaa tcgtaacgac aagccttcgc gaaggcgccg gaatccgcaa actggaggcc
     8281 ggcggaatcg gtcgcgaccc catgagtatg ctcgaggaga tctactacga tacgccgatc
     8341 gaagagccga agccgaccac tcggccggcg gaggaacaat cgtcctcggc gcggtccggt
     8401 attgacgtgg tatcgcagtt cgtgccaaca ggcgtcctgg ctcggatcga acgcgatctg
     8461 gcggcgatcc cgctggccca gatctcggtg atcagcagga acgcggcgac caagcctgcc
     8521 gtggcgccgg cgacgacgcg ccccgccgcg gcacccaagg ttggaacgca cccttcgcag
     8581 gtcacgccgc agccacctgt cccgccggcg ggcaatccgc aggttaagga ggcgaagacc
     8641 gcggtgccga aaggcgcgcc cggcccgcgc ggcacgagtg aggctgctcc cgtcaatcga
     8701 gtatcgggcc aacaagccgg gctcggcgat tcggcgaaag ccgcttcgcg ggatgagatg
     8761 accgccgagg agagacagac cgccgagctg tttcaggctg ccgccgaata ccgaatgagc
     8821 aacaagccgc agattctcta cgatgaggtc caggggactt tcattatctt cggctcggag
     8881 gacgaggttg aggagatcga cgagatcctc gacatgatca tcaaggaaat aggcgaaatc
     8941 gaagagggtg ccaagggcga tattcgcgtg ttccgcgtca agcacgttga cgtgcaggtc
     9001 gcggcctcga tgcttgaggc catgttcaac gagccggcca aggctcagcc ggcgcccagg
     9061 gcgccgaggg cccaggaacg caagccccag gcgaagcccg gaggggccaa gtcgggcgag
     9121 caggaagagg aagaatccgc cgctcagcgc cggcgcgagg aggaggagca ggccaaggcc
     9181 aaggaagccg aggaggctgc caaggccgca gcggccggga acatcaaggt cgtgcccgac
     9241 ccgcggacca acacgctgat catccgggcc gcacaggaga ccttccccaa aatcgccgag
     9301 ctgctgctaa agatcgaccg gccgggcttg gacaaggaga agaacatcaa gatcttccag
     9361 ctcaagaagc tgaacgccgc cgaggtggaa gaggtcctga aggaaatcct gaagattgaa
     9421 acaccgcagc agcggcgcac cttccggccg cgattgcccg gccgcggagg agccggcgtc
     9481 gacgtcgaca tgatcgaggc cctggagcag caaatgctgg agttccagca gagcatgatc
     9541 gagtcgagcg cccccgtcgc cgagggcgag ggtaaggaag gcaaaacaaa gatcaacccg
     9601 gccaaggaga tcagtatcgc ctccaacgcg accaccaaca cgatcatcgt caccgctccc
     9661 gaggagggca tgaagctggt cgagacgctg atcaacagac tcgaagaaca ggatattccg
     9721 atcgctattg agaccgtcga gcttcagcat gccgaagccg agaaggtggc cacccagctc
     9781 cagggggtct tcgagaccgc gcagcggcgc ggcggcggcc cgagaggcgc gatggagggc
     9841 tacacccctt cccggatggg cgagttcaag gtcgcggctg actcgcggac caaccggctg
     9901 gtgatccggg ccctacagac cgacatggag cggattctta agatcgtcga ggagctggac
     9961 agcacgccct cgaaccccgt ggagatctac cctgtcgaga aggccagcgc cgaggacatg
    10021 gccaagacgc tgagcgagat ctacggcact ccgggcgggg tcggcggcgc ccagatggtg
    10081 cgcatcaccg ccgatcgcag caccaactcg attcttgttc gggcccccaa agctcagcag
    10141 atgctcatcg ccgccaagat caaggacctg gagcagcaga gcgtcattca gggcgaagtg
    10201 cggcagatcc agctcaaggt ggcgacaccg tcgtcggtgg cgtcgcagtt gacacagatc
    10261 ttctcgccca agggtgccgc ccgcggtacg cggcaacaga tcaatattgt cggcgaggat
    10321 gccagcaaga tgctcttcgt gacctgcccg ccggacatgt tcgaggaggt caagaagatc
    10381 gccgagcaga tggatcagat caccgagcag caggtcaagg tgacgcgtct cacccacgcc
    10441 gccgcatcag agattctgcc caagctgaac gaaatggccg ccaggctcat cgcccagacc
    10501 ggattgaagg gtacgggcga cgcggttttc gcggcgacgg cggacgaacg aacgaactcg
    10561 ttgatccttg caggtacgcc caaggccttg atggtcgtgg acgccgtgat caaggagttg
    10621 gatgtcacag gttcggacac cacgcaaccg acgacgaaga tgttcccgct gaatcgaggc
    10681 aacgcggcat ccgtggccgc gaccatcaat cagttgtacg gttccaagcg gtggcaggcg
    10741 ggtgtcgaag cccccaaagc agcgcccgac ccgatcgcaa acgtggtttt tgttaccggc
    10801 acgacggcgc agcttcaatt gatcgacgac cagatcatca agcccttgga agggtactcc
    10861 ccggccagac cggtcgaggt ggcggtcaac gagtatgagg tcaccgtcaa ttacgcggat
    10921 gtcgaggaac tggccgcccg gctcaccacg atgttcaacc agggcattca agcccgcaag
    10981 tcggccggcg acaccagcct gacggcgctg gacacggcca tgtcgatcgt gcccgagccg
    11041 tcgacccgca agctgttcat cagttgctcg gcggagagca agaagaagat cgacaactgg
    11101 ctcaagggtc tggacgtcga agcggcgtcc ttcgaagggc agcagaccaa agtacttccg
    11161 gttcgcgttg ccgacctcac ctatgtgtcg aacgccctca atgccgcctt taagaaatca
    11221 gggaaggtgc ctgctagcga gcaggtgacc atccttcccg aatacggaac gaacaccata
    11281 gttgtcaaag cgctcaagaa agacatcgac aagatcgagc aactgctcca cgaaatcgac
    11341 aaagaggacc gcgagcgaat ggttgcgccg gaggcgatac cggtgaagca catattgccc
    11401 acccagttgg cgaatcgctt gacggccatg atcaggtctt catacgccgc tgacaggcga
    11461 accaaccagt atccggtcag cgtcgagcca aacgacacgg ccggcacgtt gctggtgacg
    11521 gcaaagaatc agcaggcgat ggattcgatc aaggagctga tcgccaaact cgacagccag
    11581 cccgccgagg acttgcggct gaccaaaccg tacgtgctga aatttgccga cctcgggtcc
    11641 acccagatga tgatcaccaa gcggttcggc aagcaggacc aactcccggt tcgcgaccag
    11701 gtggtcgtcg acgttgacta ttccaccggt gccctggtgg tgaccgcgtc cgaggaaaac
    11761 cacgccaagg tccgcgaaat tgtcgaggac ctggataacg ccattgtcga tgaccccaag
    11821 atgccggaga ccatcaaggt caagaacgtc cgggcctcgc tgctggccaa gacgctgacc
    11881 gacatgatca cgcagaccat gaaaccgaac aagctgaccc gccagtatcc ggtcacggtc
    11941 caggccaacg ataccgccaa cacgctggtg atgaccgcga acaaggccga tatggagcgg
    12001 ttcaagaaga tcattcaaga actggacgtg gctcccgaca cggaggacac gagggtcatc
    12061 aagccgtaca tgctcaagtt ttccgatccg agcgtggtga ccaaggcgat caccgaccag
    12121 ttcgccggac aggagcagcg gccgttgcag gagcaggtca ctgccggccc agactacgca
    12181 accaattccg tctttgtcac cgcgtccgag gagaaccata agaaggtcca ggcgatcatt
    12241 gatgcgctgg atcaatcgga cgccgccggc cgcaagacct acaccttgga cctcaagaac
    12301 gctgatccgg aagacgtcgc tcagacgctg acgacggtgt acaactcggg tccccgcacc
    12361 cggaccggtc gaacgccggc ggtattcact ccggttcccg gggctcgcaa gctgttggtg
    12421 acctgtacgc cgggcgaact cgagcaggcc caggccatga tcgagcagct cgacatcagt
    12481 ccgcaggagg gcggccagaa gcgggccatg cgggtggttc aggtcaagaa gctgccggct
    12541 cgcgagatga tgaccatgct caccgagtac ctgcgtaagc cgggcaagag cggcggccgc
    12601 gacccgtcgc tgcaggatga catcaaaatc accgccagcg acgccgccga cgcggttgtg
    12661 ctgaccggcc ccgaggagcg cctcgaggag ctccagcagc tcatcgagaa ggtggacagc
    12721 gccgtcgtcg acccgagcga tccgaatgcc ggcgggcgtc agtatgcggt catcccgctg
    12781 accaacgccg atcccagctc ggttgccacg gtgatttcgc agacctaccc ggctcgcggc
    12841 aacgtgcccg attcggagcg ggttaacgcc gtggctgagc gggccaccaa ctcggtggta
    12901 atcagcgcat ccgccgagaa gctgcagatc atccgcaaga tgatcgacga actcgaccag
    12961 cagtcgacca acgtgccgca gcaggagatc attcgcctca agaatgcccg cgccgaggat
    13021 attctcgagg tgcttcgcca gacgtaccag atgggacgtc gcggcatgtc gagctcggga
    13081 ccgccgatca cttttgccgc cgactccaac gcgaacgcgc tggtcgtctc ggccggtaag
    13141 gccgacatgg aaggaatcaa gaccatggtc gccgagctgg acaagccggt taccgacgag
    13201 gtcgaccagt tgcggatcat cccgctgcag ttcgtcgacg cccaggagac gcttgaaatc
    13261 ctgaacgagt atctccgcaa gccgggtgca accgccggac gtcgcggatc gacgtccgag
    13321 ttgatcggtg acatccggct tcaggcctcg gcgaccctca acgccctgat cgccagcggt
    13381 tcgcaggatg aactggaccg cgtggagaag atcgccctgg acatggacaa gcaggtcgaa
    13441 ggggccgggg cgcccaagat catccccgtg aagaacgccg cggccgcgac actggcgggg
    13501 acgctcaggg agctgttcgc caacccgaca caaagccgca gcgggaggcg aaccacccag
    13561 aatgacgttc cggtgatcat gtccgatgac tccacgaatt cactgatcgt gcgggctcgc
    13621 accgtggatc tcaacctgat tgaggacatg gtgcgggagt tggacaagca gactgaaggt
    13681 ccgtcaggac ttgaggttat acctgtcagc cggggcgtgg acgtggtctc cctggccagg
    13741 cagattcagg acgtcatcaa cgccggcgag cgagccaagg ccagctccca aagcacctac
    13801 aagccgcggc aggtggccat cgccggagaa gaacggtcct cgaccctgat cgtctccggt
    13861 gcgccggaac tgttcgaaac ggtaaggaag ctggccaccg agctggagca gaagcgaccg
    13921 gccacgggtg gccggcgggc gttggtcgtg ccggtcaaga ccaaatcggc ccaggatatt
    13981 cagaaggtgc ttgaggccta cattgagcag caaaacggcc ggacgcaaca gaggagatga
    14041 gtcggcttgc ttggcctgga attcgctcgg cccggaatcc tttccgggcc ggtcctgcga
    14101 agcgctggcc gcgaagcagg gccgcctcgc ttggcggaat ccattccgca ttgggcgaga
    14161 atgaggatgg aatcctcggc acggaaggcc ccagaaagga ttctggggcc agcgagaatg
    14221 aggatggaat cctcggcacg gaaggcccca gaaaggattc tggggcnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnncagc gatacgtgga aggccccaga aaggnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggattc tggggccagc gagaagggat
    14521 tctggggcca gcgagaaggg attctggggc cagcgagaag ggattctggg gccagcgaga
    14581 agggattctg gggtcagcga gaagggattc tggggccagc gtgggtgcca ctggcggctt
    14641 gcccgccagt gtagacctgg cggtgaggga gccgctggaa agcggtggtt gacaaatgaa
    14701 tctggtatac ttcgctcgcc ccgatgggcc ggcgcggccc tgacaggtat tggattgtga
    14761 cggacgttgc gcttcggccg atgtctaagt gtgaggccgg agcgggactt aacgcgtgaa
    14821 ggagtcgtcg catgtggtat tcatggctga ttctgatagc ggcagtcggg cagaccgcgc
    14881 ccccggaatc attcggaccc atagcgaaga gaacgatcat cagcggtgac cgtgtgacgg
    14941 tgatcccggt gggagaggtg acggattcac cgactacgac tcgaccggcc gcaggcgcgc
    15001 tgcgcagggt cggcaggatt cccaccgctt cccgaccggc ggcatcggac gggtcgcgcg
    15061 cgggcgatga atctgtgctc tcgcccaggc ccgccagagc cgtgccgttg acggttgtca
    15121 ccaagccgac gaccctgccg tcggaggcga ctgcctcatc gacgccgctg ccgctccgcg
    15181 ggcccgcaag tcggcctgag tcttccgtcg atctcgacgc gcttcgcaga aagcttcagg
    15241 cctcgtccgc agcccccacc gtcgccgcgc gtgccccggc cgcgcctgct ccgtcggccg
    15301 tcgagattcg ggaattgggc ccgctctccg gcgacacggt aagcatccac tcgaccgcca
    15361 ccggcgagct tatcattgag gccaacgagg aagacctcaa agtgatcgag gccttgatca
    15421 atctgcttga cgcccagccg cccgaggcgg agcagacggt cagggttttt actctgcagt
    15481 cggcacaggc caccgatgtg gcgacgaaca tccagaaact ctggaatgaa tacaagaaac
    15541 cggtctccgg ccagattcgc ccggaggatc ggctgacgat tattcccgac ccccgggcaa
    15601 acctgttgat ggtcgccacg gcgatcggca acctcaaaga ggttgagatg atggtcgagg
    15661 cgctggatca atctccgatc ggcgggaacg tgaaggtcgt gccgctgccc ctgaaacaca
    15721 tcaaggcggc cgaggccgag gaggccatca aaaacctgct gaaactcctg cagcagcagc
    15781 gcggcgcatc gaaggaattg tatacccttt atgccgatgt gcgcaccaat atgttgctcg
    15841 tgtcggcgtc ggagcgagac atcgaacaga tcaggcagct tctggacatc atcgacgtgg
    15901 agccaagccc cgagaccggc ggcgttgcga aggttgccat cgtgccgctc aagaaggccg
    15961 tggccgccga cctggtcaaa gccctcaacg atatgctcgt ctcgcagagc gacgcggcga
    16021 aggcgatgaa ggagcagatc cgccgcctgc aggttgtcct tgcgaaagat gagaaccaga
    16081 aggaactgaa gccggttgat ctcgaaaagc cgatcaagct gttcgccgag cccggcacca
    16141 attcggtgat cattgccacg gttgagggca acatcgagcc gttgaaagaa atcatctcgg
    16201 agctcgacgg tatgccgctg gcggacgaga tcatgctcag ccttttcccg ctcgagcacg
    16261 ctgacgcggt gagcctgcgg gacgacttgc agaagatttt cgaccagagc cagaaagtga
    16321 ccgacgtccc cggcaaagag gcggtgaaag gccgcacccc cgacaccatc accaagggcc
    16381 tgatggctcc gatcagcctg gtggccgaca agcggaccaa taccctgatc gtctcgggcc
    16441 ggcccgagca gcttctgctg gtccagcaga tcgtgaagac ggtcgatgtg gacaagattg
    16501 ccaacaagtt cccggttcgc atggtcaagc tggagcattc cgacgttcgc cggattcgcg
    16561 aggtggctca gcaactggcc gaccagcggc agaaaatcgc cgagcggctg ggtgaaatgg
    16621 ccaaggagcg ggagagcatt ctgctgattg aggacatccg gaccaacagc ctgatcatcg
    16681 cggccaacga cgcgaacttc caggagatcg aagacctggt caagaagctc gacggcaccg
    16741 aggatgactg gctgggccag atcaagatca tcaacctgcc cgataacctg actgcgccgg
    16801 acatcgccga caaaatcggg gaattgtggc agcggcgcgc caagctccgc caggagggcg
    16861 gcttgccggc cgacgagccg gtgatcgtgt ccgattcgcg gaccaactcg ctggtcatcg
    16921 cgtcgaacat ggaggacttc gaggccatcc agaagctcgt ggaccagctc gggcagcaga
    16981 agctgtcgcc gatgctcgac atctaccagg tgcgggtgca gaacaatgac gccggcaagg
    17041 tggccgagac ggtgcaggac gtcatggaca agcgtctcga gctgagcacc gacccgaacg
    17101 ccaaggagca gccctcggaa cgggtctacg tggtcgccga cccgctgacg cgaatgctgc
    17161 tggtggttgc atccaagcgc aattacgaag aggtcgtctc gctggtcaac aagctggaca
    17221 ctccgccgcc ggtcgacggc atcatccgcg tctattacgt ccggaacatc gacgtggcca
    17281 aggctgaaaa gaccatcacc gacctgttcg agaagggaat ctacaaggcg ggtgtcgacg
    17341 acaagaacct gcccgactcg atgaagaagg tcaccgtcat tcccgacgtt cgcagcagtt
    17401 gcctgatcgt gagcgccagc cccgagaact acgcgatcgt cgagaaggtg ttgcaggaaa
    17461 tcgaccgttc ggagacgccg atggctcagg catacgcccg gtttttcgat ctgcagcatg
    17521 ccgatgccgt gaaggccgcg gaaacgctga agacgatgct cgaggggttg aagaagagca
    17581 tgcccggcga ccaggccgat gaactgggct tcgagatcac gcctgaggtc cgcaacaacc
    17641 ggctgatcgt cgtgggcacg cgctacgcga tgcaacgggt cgaggaaatc gtgccgaagc
    17701 tcgacatcgc gcctcgaatt cccagcggga tcacgcagtt ttatgagctc aaggaagcct
    17761 cggcgagcca ggttgcaaag agcctgaccg acctctttga gaaacagacg ccaaagggcg
    17821 aacagacacc gatcaccatc attcccttcg acgcctccaa cggcctgctg gtgaccgcct
    17881 cccaggacga tcacatcaaa atgcaggaac tggtcggtct gctggaccgc aagacgaaga
    17941 tctccgacca gatggagatc atctcgctga gtgaagcaaa ggccgaggac gtcgttaaga
    18001 cgctgaccga cctgatggaa cagcagcaac gggatcgcga gggcggttat acggtggcag
    18061 ccgaaccgag gaccaactcg gtggccgtct gggccacgcc cgacctcatg gtccagatca
    18121 aggagatcct gagaaagatc gaccttgccg gtccgatcga cactcgctcc atgcgggtat
    18181 tcaagctcca cagcgccaag gctgaggatc tggccaaact cctcgatgat ttcttcaaga
    18241 acgccggcgc gggcggcagc gacgggaaga acgttcggaa gatgatcatt gatttcgtcg
    18301 cccatcgaaa tcccgattcg ggcgaggaaa tcacccagaa actggttcac caggacgtga
    18361 ccatcgtgcc cgatccctac accaactcgc tgctggtgct ggctccggcc ggcagcatcg
    18421 acatgatgcg catgctggtc gagatgctcg acagcatcga gccgcagacg atttcgctgc
    18481 gggtcttcga gttgctcaac gccgacgcgg aggaaatgag ggacttgctc gagggcctgt
    18541 tcaagacgca gggcgggggc agcagcggtt cggccccgca gatcacgctc ggcgagggag
    18601 cagcggccgg tctggccgcg gccgccggtg tgagcggcac gggcggcgtc ctggaggtgg
    18661 agtttgccgt cgacaaacgg accaacacgc tgatcgccgc cggaagcccg gcgcacttgc
    18721 gcatcgtgga gtcgctggtg ctccagctcg attacaggga actggacgag cgcatcgcca
    18781 aggtggttca gttgcgaaat accagggcct ccgaggtcgc caagactctg accgagtact
    18841 tcgatcagga aagcgagttg atggaaaagg ctttcggcga ggaggccagc cagcggcgat
    18901 tccagcggca ggtcatcatc accgaggccg ccaccggaca gatcgccgcc gacgagagcg
    18961 gcggcagcag caacaagaaa gagtcgaacc tgctgctggt gagctgtagt ccgcgaatgg
    19021 aatcgctggt ctaccgcatg atcaacgagc tggatcagcc gatcccgcaa gtgatgatcc
    19081 agatgttgat ggccgaggtg accgtggata acagcttcga ccttggcatg gagttcgccg
    19141 cccaggacct gctgttcagc gaaacggcca cgctcaacgc gaacacgggt ctgcctcagg
    19201 ggggcgatta cgatatcgta ttcggaaccg acctcggagt ggccgccggc ggcggcttca
    19261 gcttcaccgt cacgggagag gacttcagct tccttcttcg ggcgctacag ggtgagggtc
    19321 ggacggaaat cctggcccgg ccgtcaatca tggtacagga cggtcaggcg gcatttatct
    19381 cgatcggcga ccaagtgccc atcgtcaaga gcgccagcgt gagtcaaggc gttgtcatcc
    19441 cgacggtgga ttacgtcgat gcgaccgtca agctgaccgt gacgccgatt atcaaccccg
    19501 acaatttcgt gaatatgtat atcaagcccg agatcaagaa cgtcagcgca tccagccagg
    19561 acctgggcaa cggcatcaag gccccgatca tcaatacacg agaagctgaa acctccgtaa
    19621 cgatcaagga cggggagacc atcatcatcg gcggcctgat caaggggaag cgagacgaca
    19681 ccgagtcgaa ggtgccgatt gtgggcgata ttccgattct tgggagcctg ttccgctcga
    19741 ccaccaagcg ggacaccaag accgagttgc tgatcatcct gacgccgcgg gtgatgcggg
    19801 cggtcgagga tgcccgcaag ctgtccgtcg agatgcgcga ccagacgggg atcatagacg
    19861 atcgaatccg gaccaacccg ctcatgcagg gactccaggt caagccgacc gatgttgaag
    19921 gcctacaaac gccggcccag acgcgtccgg cggaccatga ggccggagag gacgaactct
    19981 tcggaccgga gatcgacgag tacggccccg ccgccagcag cgtccgaacc ggcccggtgc
    20041 cggcagtcgc catccagccg cccgtaacgg tttcgggaaa agctcggcgg tgagctcgtc
    20101 gagggacggt cgctgttcgt cggtcacggg gatgccggcg tccgtcagcg acagaccacc
    20161 gacggtggcg cgtccggcat gaggctgatc gtgctccagg ggtgcccttg aatggcaatg
    20221 atcctgacca actcgggaat ccacttccgc cgggtgggcc acttgttcat cgcggcggca
    20281 ggcactctct tggcgaccgg ttgcgttcag cctcaagagg cggcaaccgg gtcgggcggc
    20341 gcgcttgtcc aggaggtcgc ccgaatgccg accgagccca gcgtagtcag catcgtggca
    20401 ttctacaagt caactgcgcc ttggctgtgg aatgccgacc gtaccagggt ctcgggcatc
    20461 tacgtctccg ctctgtatct tctgggaccg aaggggctgg gcgtgttcgg cgacgggatt
    20521 atccgcccga gaatgttcgt ggtcgaaacg ggcaaagatg gcaagaggca gagtaaactg
    20581 gtgaaagagt gggcgttcaa cgtcgaggaa gcggttccgt ttcgcgccaa agagaaaaaa
    20641 acgctcggtt ggggttacgg cctccctctg cccttcggag atctcgatgt cagcgggcgc
    20701 gagatacaga tggtcatttg cttcgagcgc agcgacggcc gtttggtgac ctccagcaag
    20761 cagaccttcc tggtacccaa gtccaccgaa tgatggacgc tggggcttgg cttcctcgcc
    20821 tcagcggcca gtggcttttt cagtttccgg gggctttctc aggaccgcga ccatacatgt
    20881 cgggcgtagc tcgccggcga ggccgggagg gagcagcgtt tcttccacaa aatcccgacg
    20941 gaatcctgga accttcggac cgtgccgcgc gctcaagcag gcagagggta cgaacatggt
    21001 cagacaatgt ccaggcgaag aagatcgatt gcaggttcat gttccacaag aacttgtgaa
    21061 cgaaatgcgg aagatttatc cttcaggggg gcctgtaccc gcgcagcttg acgaggccat
    21121 tctcgccgcc gcccgggtca ggattgccac tcgacgacga gcttggatcg tgttgcggcg
    21181 cgtctccata gcggtaacgg cggcggctgc ggccatcggt ctggcggtct ggatcggcca
    21241 gggcggcctg aagtccaccc cctcacccga gatgatgatt gccgctgtaa cccccgccgg
    21301 ccccaaagac atcgaccgca acggccgggt cgacatcctt gacgctttca tcctcgcccg
    21361 ccacctcgac gaaaggctcg gaaccacggc cgacaccagt ggggccgacg ccctcgtcgg
    21421 cccgcaggac agcgcggcat cggcaagaaa ggcttctgga cggggcgagg ccagaccgtc
    21481 cgactgggac gtaaacgggg atggggtcat cgaccaaggg gacgtcgatg ccgtggccat
    21541 ggcggcagtc agcctgaagg gaggagcgtc atgaagggtt cagggttcag ggttcagggt
    21601 tcagtgagga aggatgaagg cggaaggcag aaggcgaaag gcggaaggca gaaggcggaa
    21661 ggcggaaggc ggaaggatga agggaaacga gttgctgata gcggttttcc gattcatcct
    21721 tcgtctttca tccttcatcc ttttggtcgc gggggggaga tcgcgcgagc aatgctcgtg
    21781 tgcgtagcag ctctcgcgtc atcggttgct ttctcggtcg tggttgctcg ggcacaggag
    21841 gtgccggcga cccagtcggc gtcgaagccg gccatccgct tcacccacgt tgatgtcttc
    21901 attgatgcca aggatcagcc cttggccgcc tatcagttcg agctggccgc cgagaagggc
    21961 gaggtgaaga tcgtgggcat cgaaggcggc gagcatgtgg ccttcaaggc cccgccgtac
    22021 tacgacccgg ccgccatgag ccgcgaccat gtcatcatcg cggcattcaa caccggcaag
    22081 gacctgccca agggcaagac ccgcgtcgcg agagtccacg tgcaagtaac cggcgagacc
    22141 aagccggaat acgtgatcaa gctcgaagtc gccgcctcat ccgaggggcg gccgatcagt
    22201 ggtgcaacgg ccaccgtcat tgaaggaggt tcacgatgaa gtccgtgcga aggattgtgc
    22261 tcgttctgct ggctgcggcg ttgggccttg cggcgtacgc ttgctcctcc gcgcagagac
    22321 tcgcatccaa ggcagacaag gaggccgccg cgccggaaac gtcgacacct ccggcccagt
    22381 acggcttcac gcctgcacct gcgcccggcc ttggcgaacc acctgcacag ttttcgcttc
    22441 ccttgacgcc gccacaaggc aactcgtcga gagaaaccgc cggcaagccc tccggagagg
    22501 atagctctat gcggtttagg ggcaaatggt ggggacagga cgctgccaag cctagggatg
    22561 cgtatggcat cggcggacgg cggtggtcgt tgttacgtga aggcaagtct cctccgcccg
    22621 gcgctcttcc ttccccagga gaggaactat ggatcgtcca gcgtccatcg ggcgagcagc
    22681 cgaggccgac cggcgaggat attcccggca gcggcgaact gagggccaag ctgcccggcg
    22741 agacgaagga aatcccgctg ccgctcaagc acaccgacgt tcaggccgcg atcgccggct
    22801 acatcgccac cgtcgatgtc actcagcagt atcacaatcc ctacgagagc aagatcgagg
    22861 cggtctatgt gttcccgctg ccggagaacg cggcggtcaa cgagttcctg atgaccgtgg
    22921 gcgagcgaaa gatccgcggc atcatccgcg agcgtgagga ggccgagaag atctaccagg
    22981 aggccaaatc gcagggctac gttgcttcgc tgctgaccca ggaacgcccg aacatcttca
    23041 cccagtcggt ggccaacatc gagcctggca agcagatcga catcaacatc cgctacttca
    23101 acaccctggc ctacgacgac ggctggtacg agtttgtctt cccgatggtc gtcggcccgc
    23161 ggttcaaccc gccgggcagc accgacggag tcggggcggt tggccgcggt gaccggggca
    23221 tctccggcca gaagaccgag gtccagtacc tgaagcccgg cgagcgcagc ggccacgata
    23281 tctcgctgtc ggtggccatc gatgccggcg tggccatcga aaagatcgac agccgcaacc
    23341 acgtgatcga agtgaagaag tcctcgcccg aaaaagccga agtgcgactc ggaagacttg
    23401 acacggtgcc caacaaggat ttcgtgctcc gttaccaggt ggccggccaa acggttaaat
    23461 ccgcgatcat gacccaccgc gacaagcgcg gcggcttctt caccatgatg atctacccgc
    23521 ccaaggatct cagcaacctc aagcgcaagc ccatggaaat gatcttcgtg ctcgattgct
    23581 ccggcagcat gagcggtcgt ccgatcgagc aggccaaggc cgccgccgag cgtgccctgc
    23641 ggctgcttga gcccgacgac accttccaga tcatccgttt ctcgaacaac gcctcgcaac
    23701 tcggccccaa gccggtggtc gcaacgcccg acaacgtccg ccggggcctg gaatacctca
    23761 agtcgctcca gggcgagggc ggcacgatga tgatcgaggg catcaaggcg gcgctcgatt
    23821 tcccgcacga cgagaaccgc ctccgcttcg tttcgttcct gaccgacggt tacatcggca
    23881 acgaggaaca gatcttcggc gagatccaca agcggctcgg tgcagcaagg atcttcagct
    23941 tcggggtggg ctcgtcggtc aaccgctacc tgctcgacgg catggcccgc atgggcaaag
    24001 gcgcggtggc ctacctcggc ctcaacgaca gcggcgccga cgtcatgaac cgcttcttcg
    24061 agcggatcag ccatccggcc atgaccgacg tgatgatcga ctggggcggg atgaaggtcg
    24121 aggacatcta tcccaaacgg atccccgacc tcttcgtcgg ccgccccgtg ttgatcaccg
    24181 ggcggttcag cggtaaaggc tcggccacgg tccgagtcaa aggccaggcg ggcgacaagg
    24241 cccgcgagat cgccattcca gtggacctcg atgatgcatc cgccacgcac gaaggcctgg
    24301 ctgccgtctg ggcccggatg aagattgccg aactgtcgca gcatgcgatc atggatccgg
    24361 gcgagcagtg gaccggccag atcaagaccg ttgccctcga atacggcctg atgtccgcct
    24421 acaccgcttt cgtggccgtc gattcgctga ccaggaccgc cggcgacagc ggcacgacgg
    24481 tagccgtgcc ggttccgatc cccgacggcg tgaagtacga gaccacggta caggaacggt
    24541 agagcggact ctgatcgccg cattcagtca gaacccgcag cgcaagcgac gggccgacaa
    24601 ccagcagagc gacgaggaat cacgaaagtc gcagatcggc tgcggcgggc ccgaaggatc
    24661 ggaaacacga aagaagttca gaatgcgatg gtgtcgcggg acagcagggg tcttgatttg
    24721 tctgatcaac gtgggctgcg ccattccgat tccgtatctg tttccgcatg tcagcaggac
    24781 gcccatgatc gcctctcctg tgcttgcgga tgacgtcaag gcctttcgag tggagtatac
    24841 gtaccatcaa cacgggtgtg tcacgttgga acggcaaccg gccgaccggg tggagatgct
    24901 gcaagcccaa ggctctccgc ccgctgtccc gtcgcaatca cgattgagtc tggcagtggg
    24961 catctatctc ttcaaccctg ctctcccgca caatttcggc tccgatggtt gtgttgacgt
    25021 gcgcctttat cgcaggagct tcgagacaat ctcggtgcac ggacactcga ccaacgaggg
    25081 cctccgttcg gctcgctgga cgccggtctc cgattaccag gcccgcgaaa tcgccattga
    25141 tatggtgttc gttctcgccc cgggcagatt gttaccggtc gattcatgtc cgccggatct
    25201 cgttcggctg aaatgttcca ctgaggctgc ccgattcgcc gccgacgaat acgatgccgt
    25261 ggcaaacgag cccggaatcg ccgcaaagga cagggagcgg atcacaagaa aggcggagta
    25321 cctgcgcggc ctcgccacca agggctgttg accaagggga tgccggcgtc ctcataaccg
    25381 cgatttccct gtttcgtgct caactgatgt ggtggttatc tttcgcggtt ttcacgccgt
    25441 tgttggaatc ctcagcgaaa gcccgtggcc actcgaaccc ccggccgcaa ggactcgcat
    25501 gtcacatcac gccaaggtcc ggaaatttcg caagcgttga atggggcgaa tacctaagga
    25561 ttcgcgcgtg cgaaccgata gtgagaacag tgatgccgcc aggacgcggc aggtccgata
    25621 gtgcgagggg tttcggggcg gtgggttgcc ggaaaaacac tgagacgcct cgatcggcac
    25681 aaagtccgct ttctgcaacc ccttgaattg tatacttctt tcaggcgaaa cggcatgcgg
    25741 cgacgccttt cgcggtgagg gtccgccgat attcccgaca aagcggggga gccttttccc
    25801 cggcgaaacc ggagtgatga gtacgaggac agacagcacc gatcgtgagg atagccggtc
    25861 tgccgccgtg gcggtcgact ctgcgctgcc ggagaacggt ccggagaagg cccggcctgc
    25921 ggtcagtgat gaggagtcgg aaaacgcacc cgcctccccg gccgaagaag ttgctgccgt
    25981 ccagccctca accggcacgc cggcgcagcc cgattctcat aacacacccg aagcctccaa
    26041 ccacgaggag cggcccccgc acgtcgacga cgaacccgta ggactggcgg ccgtcgaagc
    26101 cgagctggtg gccgaggtcg tggaggaggt gtgcgaggag atcgccgagc cgtcggacac
    26161 cggttggcag ggggagttgc ccgacctcct gatgcgcatt gtggacgcgc gaatcagcgt
    26221 atggcagaag ctgttggaga tggatgcccg ccgggccgag atggagaagc tggccagagc
    26281 tcgcgagatt cgcgatgagc tctcgcgaca gaacagagag atcaagaaca acccgaacgc
    26341 tgaagttttg cagagggccc tggagcgcca gcagcagttg ctgcggaatc ccgtgatcaa
    26401 gagccaggtt cctccgccgg acccgaaagc caagacgccc gacgtcgacc agaatcagtt
    26461 ataccgcaat gccatccaga tgggcctcgg ccagatcgct ttacttcttc atcgcaggaa
    26521 gctcgatgac tcggtgccga cggccgccgt tactgccatc gcggacgaac cgcttgtatt
    26581 gctgtgccgc aaccacggcc tcaacgccga cccgctcatg ggttggacct actacgccct
    26641 gggtgttcag aaacggattg atgagtttgt cgcggcagag aaagaacatc gggcaaagct
    26701 ggctgaggcg agggccgaag acaaggagaa caacaggggc ttgggcggtc tgttccgagg
    26761 aggcagcaaa gagaaccagg ttggtgagct gagtccgacc gttcgggccg ggctgcaggc
    26821 ggcgagccgc gagatgcagg ccattgagcc gcaattgacc gagatgttct ggcgcttgta
    26881 tgaggacctc gcctggttgc tggttcaggg cggtctcgac gagaaagaga tggtggttgc
    26941 ccgatcgttc ctgcgatacg gtctgatttc ggtccatccc ggcctgctcc agccggaaaa
    27001 agtcgacttc atcatcaagg attgccagga ggacgtgtat gagtggcaga accgtcccca
    27061 gtcaacgcat ttggtttacg cggacgaata cattgaggca gtggcctcga agaatctgac
    27121 ggtcagcccc gatgagaatc tcgagctcaa cgaacgaggc agcgactcgt ggaaggcgga
    27181 ccgcgtctgg cggcaggcgg taatctgccg ggtgcgcagc gatctgttca acgctcgact
    27241 ggctgaactg aaggcgctga ttgaacggaa gcaggccgag atcgagaaga aactcgaaga
    27301 ggccaaggtg ctccgcgaga cgccgaaaaa agtccagcag gccaaggagc ttgagaaaca
    27361 ggtcatgatg atcaagccag cgatcgcccg gttgcggcat gctaccgagc acatggaagc
    27421 caggatcatc cccaaggtgc aagaccaggc cgacgaggca accgagcgac tgggtgaggc
    27481 ggccaaagtg ttcactgaag agatgctggt tcggcgcgag gcgcgtttca ttcgctatat
    27541 ggcccggttg gtcggacgtc tcaaggagcc ctttgcccag tttgtgttgc gagaccattt
    27601 caagccgaac ggacctgacc acaacagccg tcgcgttgtc ttggaagcca tcaagaagct
    27661 ggaaagcgaa gaccgccgtc tcttccatca cgtgctgatg ccgaacaagc ggcttgatcg
    27721 acaaatcacc gtccgcatga gtccgacctt cctgcttccg ccgggacgag ggcagatggg
    27781 tctggcggtc tcgccgcgga aatgggatga caacggccgc attgttcttc cgttgcttgc
    27841 ggccaaggcc ggtgatctgg agaagttgct ggttaacgtc atggcggact tccggtggga
    27901 ttgttcaaag gaggacgcgg gcatggactg gatcgtggcc gatgctctgt gtgccgccta
    27961 cgctgccgcc cgctggaacg tccgcaagct gccggaaaag gcccagaaga tgatgggctt
    28021 cgacttcaag cttaaagagc ggggtaattt ccgcatccac tatgccctct acgtcaacag
    28081 cgcccatcag ggcggccgcc tgttgttcag ccggagcgac gaggtttaca aggtgatggt
    28141 caagtacatc ggtctgccgc ccggcgttca aatgctcaaa cgcgattgag tctgcgatcc
    28201 ggagcccggt gtacggccga catcttgccc gttccccgag agtcctgtga catcctcaag
    28261 gtgcgccggc aacccgtctt ggcctgcagg tcttcctggt cgcgcaggct cgctgggcat
    28321 cgacctcaaa gaggcggatc gctctcagga cgatcttgcc ttgccgaagt ccggtgcata
    28381 tcctgtcgga tagtcgctat gagggatttc ccatcgactg acccggttgg gtcggacgcc
    28441 ggcctgatca gaaggtggtg gcttcgtttc tcgtctctta ttgagggagg cactcaatca
    28501 tgcgaaggtc ggttttgatg attgtcggat tggccggtct ggccttgctt tcagaggggt
    28561 gcatgagggc agccagcgag ggtatggaag ctgtgcgagg cggtcagggt gcttatttcg
    28621 aggagaattt gctctcggca acgccgaagg atcatctcgc cttgtcaggt tatcagagaa
    28681 tcgagttggg cgagttcacg aaccagagtg gtcaagccat gccgccggaa ttcccgacgc
    28741 tgctggccgc gaagttcaac gagcgccttg ccaaatcgga cctgcccaag gaaacctcgg
    28801 gcaagacgct gctcttcaag gtgacactga tctactacga gaaggccgac ctcaaggaca
    28861 gcgtctttgg acccctggag gaggctgtgg cgagggccga actcgtggac aaggataccg
    28921 gcaaggtctt ggcgagcggg gccgtggtgg ggcgctccag ccagagcgtg ggtcttggtc
    28981 cggcatttaa ggctgacggt atggccaagg ccctgatcaa gtgggcatcg gactactatc
    29041 cgaagcccaa gtagcagacg gtcattgttc cttcgttctt gccgggcgcc ggcgatgctt
    29101 gagcacacgc cgggacaatc aggcgacgtc cgcacccctt cgcgacgcaa cggcgggcga
    29161 tgggtctgct tgcggagcct ccggaacgcg acgagcgggc gactcgattg ccatgaactt
    29221 cctcatctca actgatccat gcccacctcg gtgcggatct tcttgcgcag ggcaaacacc
    29281 ttttcgccgt ccatgctgcg aacggtccaa tagaactcac gcatcgccga cgtctcgccc
    29341 gcaagatcct tgtagatttc ctccagaatc ggcgtgtgct cgtcgtcgtt gagagcggcg
    29401 atcgcacggt atacctgccg gcgccacgtc gaatcctggc gagcattctc tcgccagtgc
    29461 tcgaccagcg gttccagccc ttccggttgc ttgagcagtg ccagcaggta tcccgcatgg
    29521 gctcgggttg ttgcgtcgcc cgaccggagc aacggacgaa cgttatcaac cgtcagccct
    29581 tgaggcgcct gcggcgagat cggttgacct cccgaagagg caaagtccag aaaggcggct
    29641 tctgcctccg agaatatgtg aagggacaat acgcccctga gcttatcgag ttgcgtcttc
    29701 cctcgggcaa ggtacagcaa ggccgtaccg cggacatggt cgtcgggact ggccagtgcg
    29761 gtcgctgccg tctcaaccgc ctgtgcctcg gattgcccca gcaacatgat ctgcaacgca
    29821 tccgcgcgca acgggctgtt cgattcgggg gcctcgtaga gcctgcgggc ggcggtcacc
    29881 gcgtcggact tcgaacccgt gagcagcagc gacagggcga caagttgccg cacttccggg
    29941 cctttctccg tcatcggcgt tgcttccgtc accaaccgcc ggcgtctttc ggcgttgacg
    30001 tcggaccatt cgtactcggg cccgaggtat agccgcagaa gactggcttg cgccgtgtcg
    30061 gcccagccaa gggcgaagtc cttctgaccg agcagttccc aaagcggcgc cgctgcctgc
    30121 ggatcgggca ggatggcaag accgttgatg acgtcgacca tgagttgagt ctcagcggcc
    30181 agacccagca gctcgcggaa cagggatacg cgctcgccga gcggcagcca cccgagcgcc
    30241 tgcgccgcct ggctgaccgc ggcgttctgt gagcggacta cgtcgcgcag aacagccagg
    30301 gccgtctcgt cgcgtccgag cgcaatcagg ccgatacagg cggtcaatcg gtgctcccac
    30361 tgcgagctgg acagtaaccc ttccagcggt gcaacggcct gatccatcca cttgggtcga
    30421 tcgatgcccg atctgaaatt cgccagccaa tcggcatccg ctaccggctg actctgcgag
    30481 ggcgcgcttg ccggggatga ggaggcgacg cgcacaacaa cacgcctggg tgacagcccc
    30541 agtaaccgag cgatcagaga cgaaccagtt cctgcgattt cttccgtccg atactcatat
    30601 gacgtgaacg gcgtttcgct tgggcgacag gacgtgagga ccagaatcaa ccccttggcg
    30661 gccgcgaacc gaacctcttc ttccggatcg ccaagagccg tcaacaattc cttctcggac
    30721 tgtctcggga gcactctgca aaaatgctcg atggcggcgg cgcggatgct cgcgtgtttc
    30781 gcgctgagca gcttccgcag gtacgtgccg gtctgttccg cgtccccgcc tcgacgggtg
    30841 agcatctcga tcagggagcc ggccatctcc ggatgatcca gcgcggcttg gaccagcggt
    30901 tccgtcacca ggctcacatc cgcgtgatca aggctggtca tcgcccggct ggtgacaaag
    30961 ccgtcgggat ccttcagtag agcaagcagt gtgacataag catcagcccg cgattgccgg
    31021 ggcagattac ctgattcgat gaggacctcg ccgattgcct cggcgacctc ggcccggacc
    31081 tgccaggcgt cgtgaccggt catccggctc agcgcttcga gggcatcctt tccgccggcc
    31141 gcgcgcaagg cacggacggc atgaaccagg aggtcggcat cctgctcagc ggccgcatac
    31201 tcggcaagcc tggggatcag ctttggcgaa ggctgctcgg tgagctgctt gagcacggcg
    31261 gcccggacat tgggctccgg gtcgccaagc aggctcatca gggcctcgtt agcttcctcg
    31321 ccgcccacct tgcgcaacgt tcgaaggctg atttcccgaa ccagcgggtc cgggttgctg
    31381 aacagctcgc gaagcaaagc tgaagcatcc gcctgagccc gagaggcgag ttcttccgcg
    31441 gcttgctgcc gcgtcggagc gtcggtcgaa gccagtcggt caaaaccacc cggccactcc
    31501 agagccaatc tcgtcggggc ggccagccga tatcgcaagg cggtgagccg ttcgcgatcg
    31561 cgatccgtgg cggcctgctg cagttcccca gcgacaaccg gcagcaacga cgggcccaac
    31621 cgcgccagcc gttctcggac gatctcggcc tcggcgtcgc gatcggcctg gacgagccgg
    31681 atcagctcgc gccgggcgtt ttccagaacg tcatccggca gttgcgccgg catagacgcc
    31741 ggcgcgtcac tcatccgccg agcccatgct cgcaaggctt cgagacgctg cagcgtgatt
    31801 gtctcgggcc gccagggatc aagatcccgg attggcgccc tccatccgtc gagcaactcc
    31861 agcgccgcca gtcgttcaga caacgcgcct tcggcgaaca gtgtgacgac ggcgggcgcg
    31921 gcaagcttcg gctcggccgc gagcctttgt atggcggctt ccttcaccaa cgcctcgcga
    31981 ccgcgcaact gatcgatcaa ctcgtgcagc gtcttcgcgt cgagcggtcc gtcctttctc
    32041 aggccggggg gcgtcacggc tgtggctacc ggcaattgcc cgctcagcca ctgcgccagg
    32101 tcggcagagg ccatgtaccc atcatgtgag gcgaccaccc ggccggcgac gttcaacacg
    32161 cgcaaggccg gaacggcgtt gattcccagc gaccgcgcgt cgcgcggtga gtcatcgacg
    32221 tcaaggtaaa cccgcacgta cgacgacagc gattcctgta ccgatgagtc cttgagctca
    32281 tcgttgagtt tgcggcacca tccacaccat gttgctccca cacggacgac gatcggacga
    32341 ttcgatcgtc tcgcctcagc atagccctcg ttcagattcg tcagccagcc cgcgctgccg
    32401 ccatggagcg taccgccggt ggtattcgcc ttttccgcag aaggggtctc ttcactggtc
    32461 gttctctcgg ctgttgccga aagcaccgga gcggaccgca ggaagaacac gagaccaact
    32521 gctatggcct gcccaatcac cgcaaagcgg ctcggatgct tcatggctgt gtctccgtgc
    32581 ctgacggccg cggaccgagt cggtccggct gggtgccttt ggtttccccg tgtttgatct
    32641 tttcccagta gcgttcatac ccgaacgcag ggctgttctc ctggtcgtga caacgggtgc
    32701 actgatcctt cgcatcaaag ggcgtcctgc gttccggctc ctcgacgtga acttgcgacg
    32761 ggccatgaca ggcctcgcag ccgacgccca gtcggtcggg actcttggcg acggtgacaa
    32821 acccgccggg cagtccatag ccggtcgtgt gacactgctg gcaataggga tcgacgtgcc
    32881 gttccattgc cttgaggtcc tcccatgcct tggcgtgtcg cgacgcatcc cacagttcgt
    32941 tgtctctttc gtggcattca cggcaggttg cgctgccggc aacgcggtaa ccggggggta
    33001 catccggcgg agccgacggc accaggtctg tctggtgggg ctcgaaggac ctgcggttca
    33061 gctcttcgag gtatcgtctc aggttggcca actggccgcg atcgtcgggc cagtcccccg
    33121 tcagctccac gacttgcccc gtcggtggtc cggcgttacc cgcggccgcg aattccagtt
    33181 gcacgaggaa cttgcccttg ttggttgccg ccgccaggac ggttggaccg accgcccttg
    33241 gcgcaatcgg ctgcccagtc gggccgccga tcaccgcgtc aacctcgggc agctccgtgg
    33301 ccaggcgttg cagttcatcc tccggcaggt atgccatcac cacgactccg tcatgccgtc
    33361 cctccagttg ttgcagcgat gccagcaccg ccgccttcgg gtcttcgatg cgcacgtcac
    33421 cggcggcgta tctcggagaa atcacgccga taacggccag ccttcggccc gcggtctcga
    33481 tgatcgtcca cccatcgaac accggccgcc ccacagaatc ccgcagattc gctgagatca
    33541 aacgagcacc cgtcttgcgg gacgcttctt gaaggttggc cagccccagg gcaacttccg
    33601 gcccgccgat attgtgggcg gcgagcccca tcagccgttc accctcaaga atggcctcga
    33661 acttgagccg ctgatatgga gatgtgccgc cgggagctcc tccggcatcg aggagaataa
    33721 catccgcttc ttctctttgt ttctcgacat acgtccctcg ccgcagcagg ccgcccgatt
    33781 gatgcgtggt gcagccgcag gggatgatcc aaccggccgt gtcgccgctg accacaacca
    33841 cgacacgcct ctcgttggcc ggagggctct tttcctcgcg gcaggaccac aaacaagacg
    33901 ccagcaccac ggcgcagacc ccgatgacca ggcatcccga tcgaagcggc ttgtccgcaa
    33961 gaaggagcat actccttgat ccgctcagtg ggttaacgca tatgccagaa cgttcacgtt
    34021 gacgtcgacg gcgttggtga tctcgtttcc cccgcagcaa cagcagccct gtgtgtgttc
    34081 ggtgtcgttc aggccgtcct gcgagtacag aatcgccagc cttccgccga cgtgaattcc
    34141 ttccaggggc cgcggcttgc cgtgcttcgc gttgaggctc ttgatgtccg cgaccatatg
    34201 aaaaacgggg tgttccatat cgatgatctg cagcggcgtg tccgggaaca ctttcgccat
    34261 ctcgtttcgg aatgaccgat cccactctct cgacgagcag cccgccgaag ccaggaggaa
    34321 gccgccgcgg ttgacgaact tgcggagatt ctcgcgctca gcgtccggga ggacaaaatc
    34381 gccctcgccg gtcatgatga ccagcggaaa gctgaacaac tcgcccgacg caagtttgac
    34441 cgcatgaaac cgccggctgg tcgagatcgc cgacgacttt tccgcctgaa cgagaaagtg
    34501 atcggaaaag cacttgctgc tcttggttcc ggcgtacacc aggttggcga cctgcacgat
    34561 gctctcggct tcgccgccga cctgcttggg atcgggaacc actcgcggtt gctgcccgtc
    34621 cgccggctgc gacgccggcc gcgacgcagt cgccccgcca gacgcggcag agagatattg
    34681 ggcgaatcct gccagagctg ccagacagac tgctgctatc aggaacccct gcggcaacaa
    34741 ccccttgccc agcgagaaca ccgatctgcg agtcatgttg ccacctcgtc actaaaccct
    34801 gaaccctgaa ccctgaaccc tatt
//
LOCUS       DAOTPW010000021        34320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1352_length_34320_cov_6.480782, whole genome shotgun sequence.
ACCESSION   DAOTPW010000021 DAOTPW010000000
VERSION     DAOTPW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 34320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34320
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1352_length_34320_cov_6.480782"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="P5316_03185"
     CDS             complement(<1..430)
                     /locus_tag="P5316_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HRR83990.1"
                     /translation="MATIPALDLKKLAIMGTYVPRRCGIATFTSDLAEAIAAELGNKG
                     EVFALALDDIPQGYRYPERVRFQLRANYQPDYRLAADYVMGNQANVFILQHEYGIFGG
                     PAGSHVLRLLRELRIPVIATLHTILQEPTADQRRVLEELAQ"
     gene            651..2024
                     /locus_tag="P5316_03190"
     CDS             651..2024
                     /locus_tag="P5316_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRR83991.1"
                     /translation="MVTSQRYPDLPDASFIPRAYTGLSRQQVSELRHRYLSPALLLYY
                     SNPIMIVEGRMQYVWDDTGKRYLDAFGGIAAVSIGHCHPHVAKAVQEQVNLIQHTTVI
                     YLHPTIARFAEKLAATFPAGSGLQVSYFTNSGSESNDLALLMCRLYTGRFDAIALRNG
                     YHGGTHATMALTAQSTWKFPIPHSFGVHFGMPGYCYRCPMGLHYPACDLKCARDVAQT
                     ILYETPGEIACFICEAIQGVGGVIVPPPDYLRIIYEAVRKAGGLCIADEVQAGLGRTG
                     ETFWAFENWGVVPDMVTMAKGIGNGAALGAVVTRMDVAKGLAGRLHFNTFGGNPVAAA
                     AGLATIEAIETENLRENARQVGAYLKSRLLEMQQRHPLIGEIRGMGLMLGMEFVRDRE
                     SREPAKDEAHDVAELAKEHGLLVGTSGIFGNTIRLTPPLCFTKDDADFAADCLDQALA
                     EVQARAR"
     gene            2211..2789
                     /locus_tag="P5316_03195"
     CDS             2211..2789
                     /locus_tag="P5316_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR83992.1"
                     /translation="MMDAVVLDDLIRRARQRDSAAFETLVEIYSPRLYGYFYRLTGRR
                     EDAEDLLQEAFVRVVRMISRYEHDNRFDAWLFRIATNLVRDRVRRQRRSADAGASGAR
                     QDEPGILEEVADNEVDRPDDVLETTEQVDRLQWALKQLPEAEREVILLRHFSQMSFRE
                     VAEAMGTPLGTALARAHRGLAKLRRLMNEDAA"
     gene            2889..3494
                     /locus_tag="P5316_03200"
     CDS             2889..3494
                     /locus_tag="P5316_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83993.1"
                     /translation="MSDELNELEREVEQEMALLPSLLVEPSDATVLRVKAVVRHELNE
                     EWLAGQAAPLPSAEAVRRVHQAVIAELAKPASARLSAWGKTAAVLAAAAMIAISVGVI
                     RRTGLVGSPSVSPEVAEAEKHVELFLATAEKVFSLDDLTESIADDLSAIEEDIDGWQS
                     AGSGETGGLGDVLREIDGTTPEPLDNHGVSHLSTVSQGAWG"
     gene            3491..4378
                     /locus_tag="P5316_03205"
     CDS             3491..4378
                     /locus_tag="P5316_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83994.1"
                     /translation="MKWATAGLLTAVLGGLLGLFGPADSNESQSPKPRDRGASAIGRP
                     AETSDGLEIEQGVRSPAWPRDAWPRRDWRGRDGTVGPPDRSRYRQGMGGRSWDDEDDE
                     QMSPEQVAELMNFAEREFPKLHRRLVSIRETDPTLFRKMIRRVRGPVSEIVRIQQHDP
                     KAARRLIDAHRIEIDLTELQSQYQAAQNDAQREQLKATMRELVVKRCELRLQRLKAEI
                     TELEKRLEHAKKEMANRERDQDQIVDQELSRLLTTGTASPGGGRLWPSGELGPGFRGG
                     KRGPGPPSTGPSGTVAPIE"
     gene            4860..6368
                     /locus_tag="P5316_03210"
     CDS             4860..6368
                     /locus_tag="P5316_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRR83995.1"
                     /translation="MSHAVFCSLCLSLLTSPLYGVSSPSIVLLYADDLGYGDVGCYGA
                     TRIKTPNIDRLAQQGLRFTDAHSSAATCTPSRYSLLTGEYAWRRKGTGVLPGNARLII
                     EPGRTTLASILKKAGYTTGAVGKWHLGLGDDKLDWNGEIKPGPLEIGFDYCFLIPATG
                     DRVPCVYVENHRVVGLDPADPIKVSYDEPIGDEPTGKEHPELLKLHPSHGHDQTIVNG
                     ISRIGYMSGGKAARWVDEDMADVITDKAVAFIERNRDRPFFLYFATHDVHVPRVPHPR
                     FVGKSEMGPRGDVIVQFDACVGRVLDALDRLKLTENTLVILSSDNGPVVDDGYRDQAV
                     EKLGDHKPAGPWRGGKYSNFEGGTRVPFLVRWPDRVKPGTSEALICQVDLIASFAALT
                     GQSLGRDDAPDGMNVLPALLGESKIGRDHLVQQAGALSLRKGTMKYIAPSKGPKVSRY
                     TNVELGNDPAAQLYNLADDPGETRNLAPDKQQETQELGMSLQRLQDSGRSRP"
     gene            complement(6439..7725)
                     /locus_tag="P5316_03215"
     CDS             complement(6439..7725)
                     /locus_tag="P5316_03215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR83996.1"
                     /translation="MILDRLVAFPESRNILRLQLWATPFIALQAAVFQMLPVLLRSYF
                     QASEWETVISTSAISITLVLSVVWNELYRRIGSGRYLALLWISAIAPLAGIALCHKAW
                     TSLVFIVIAATGFGGMQPLTGDILRSCYPPASRNRIFSVVQMVTQFTVMVGTYLIGLW
                     LNWRPDSFRWYLPVGTVLVGMGMWLLYCITRRQLFIERQQAKPSERLRTSLRRVYHNM
                     LRVFREDSIFRRHETAFSIYGLGWMICWALLPFLCVDKLKLTYEEVARSTQTVLQLTL
                     LLTMLPTAYLMDQLGPLKTAAYSFAALALYPIGLMWAYDVNSLMIVTILYGVMLSGVN
                     LAWTLGPIFLARDASQAPTYLAIHATMVGIRGLIGQFPAVALYRWTGDLRMPLALAVV
                     LFALGSVIMFRVERDRRKGMAVAPVPEPIPVAPPSA"
     gene            complement(7784..8776)
                     /gene="dusB"
                     /locus_tag="P5316_03220"
     CDS             complement(7784..8776)
                     /gene="dusB"
                     /locus_tag="P5316_03220"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRR83997.1"
                     /translation="MFSLGSLRLISNLLLSPIAGYCDLAFRLVVRPLGGLGLACTDLV
                     NPRGVLRQTGASMALIRTEPDDKPLCVQFYGANPAEMIDAAKYCRDHGADVIDINMGC
                     PVAKICTRGGGAAMLSDPASAVRLAAAVVRAVDLPVTAKLRLGLDDSSMVAPQLAADL
                     ERAGVAAVTVHGRTAADKFGGKVRLDGIARVVEAVRSIPVIGNGDVRSPADAKRMIDA
                     TGCAGVMIGRGALSDPWIFRDSHAYLTTGILPPPPTVRDRLDLVVRHFEQLKRIKSER
                     VACAIMRQRFSWYAAKLQPSPEWRHRMRSVRSGEEFHHLMHEYPLSEGTDPVIP"
     gene            9152..10870
                     /locus_tag="P5316_03225"
     CDS             9152..10870
                     /locus_tag="P5316_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83998.1"
                     /translation="MRHLQLWMALVVTWSLSHAVSAGAGARYRMLSSVARRLPPVIEC
                     RITVGPTETTPDGTQGQWWSIRFQKEDEDFQIWCLSEQVPMLAALPATSPGRVWRYIY
                     REGHRPILEYRRESTGEVLLPGFGWQDHFLPHPKRATERSSGWLVSGRCMGHVIGLLE
                     TQESAEWDQPSPQEVTMLPLDDGLLVATCLSKRDINYGKKPDGDYTYRRWDEADVKAV
                     IEAGFNMMIVHSPELEDWVWKQPVFQLGAPDPYPQMFFRSAYLGSHFFIDEPAHHVAT
                     REQRRFFADIPRYDLMYDLLASFTEVALKEQSSHIMRPGSACLMGKAVPDVNWGTLEV
                     RNELLPAWETHCPAAFCEMDAGARGVVIEGRFQRPAFNAQITELLGEDMNVNAEEMLA
                     WHVAFLRGAARAFKGEWGMSIYGQADPEIAKLAVVNAYKLGARYIWFWTNDHDHHVPF
                     ERQLELARLVTDAKRNDRQPRNLARLRSAATAAVVLPTGYIFTMGGPLLGNYSLSTDS
                     KNAATAPRRAVQQTIVRTGLELMRNGVTFDYVNQCPHLASLGYDRLLHIQPDATLHET
                     KNGSLP"
     gene            11347..11637
                     /locus_tag="P5316_03230"
     CDS             11347..11637
                     /locus_tag="P5316_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR83999.1"
                     /translation="MASSRRGDLMEEGSFHKTLSKLVSEIKTMPEVEKSKLAKLAEET
                     KHRHEQLKKTVSSLQESIDFLRLSIKYLLFDLEATKRENVQLRKMLEERSDS"
     gene            complement(11972..14374)
                     /gene="bamA"
                     /locus_tag="P5316_03235"
     CDS             complement(11972..14374)
                     /gene="bamA"
                     /locus_tag="P5316_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRR84000.1"
                     /translation="MTRQHATRLGNDRRRWLCLLLGGLVIFPLTSPPLVAQVPATLPA
                     GPASPLDGRQIRQLNITGLRRIEEAYVRNRIRTRAGETYSQDQVERDRGQLLRTGRFL
                     DVKAEPVLVDGQINLTFSLVEKPEVEALEIVGNQKIKTKDLLKELTFAAGDPLDMHEV
                     RQGRDTIERLYREKGYPYVSVTYDENLLAAERRVVYTVIENQRVKIRKVRFENNVTYD
                     AGELMRQIETKSYIPIFRTGNFDADQASRDAATLQKYYRDRGFLDAEVGYVTEFQDVA
                     REKLDVIFRVNEGVRYAVAEIRIQGNQVFTAEELLNEMDLKTGDPLIDARLKSDVKRL
                     ETKYGSQGYIDVKVTASWVFASEPAKVILTLSIREGEQFTVGWIEVDGNERTQEKCIR
                     REVGLYPGDIYDVTKMTKAEQDLKATGLFIGATVEAVEPSAPQPGVRDMRVTVEENTK
                     TTNFIAGIGASSDSGLVGNIVLENTNFDLFDTPRTWDELFKGRAFKGAGQTMRVQFEP
                     GTEYTRFRIDFREPYLNDRPIGFNTGVFLFERPRDAYTEQRAGVYVSFDKKFEEGLLK
                     GWLGEIALRPEYVNVDDVELFSARDIQKVKGGSYLSTIKFSLVHDTTDSRLDPGKGHR
                     ANLSWEQAGVMGGDYTHSKLTAGYAQHWTVAIDEQDRKSVVSARANIGQILGDAPVFE
                     RFYAGGIGSMRGFDFRGISPRSGLKKDRVGGDFMLLTGAEYSFPMYDKVIRGVFFSDM
                     GTVERDFGISSWRASVGCGVRLTLEIFGTVPMEFDLAWPVIKDSEDDTRVFNFFIGLP
                     FF"
     gene            complement(14919..15461)
                     /locus_tag="P5316_03240"
     CDS             complement(14919..15461)
                     /locus_tag="P5316_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRR84001.1"
                     /translation="MPNYALLTASGPDRPGIVAAITGVLLESDCNIEDSQMARLASDF
                     ACMLMIRMPEGMASRQLEDRLSGVAGELGLRMYLQDLRPEEAREARSSQSKHLIHVYG
                     ADRKGIIHRISKRLAEFGVNITNLHTEVIHHAQPLYVMLLEVEIPPSVDAGRLQDDLM
                     AIGREIEVTVAMKPKDDARF"
     gene            16503..18089
                     /gene="rny"
                     /locus_tag="P5316_03245"
     CDS             16503..18089
                     /gene="rny"
                     /locus_tag="P5316_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRR84002.1"
                     /translation="MGLPFTLAVADYVPALLVISSLITGAVATYMGLRIFGKQKLATA
                     QRDAAQIIEKAKNEAEVIIKRAEVDAKGEFIQRMDEFEKKSAAIRDELKEQERRLEKR
                     ADNLDKKLDTLVTKERALEQAEQRLSSREASLAQKETQLTEILEQQRAQLLRITGLSI
                     DEARNLLLNQIRHEVEHDAAEMVDRILTEARETAENKSREIVVTAIQRYASEHTSVNT
                     VSTVDVPSDDMKGRVIGREGRNIRAFEKATGVDVIVDDTPGVVVVSAFDPVRREVARL
                     SMEKLIHDGRIHPSRIEEVVEETGAEVAREIVERGKKAAVEANVGGLNRKIIDLLGRL
                     SYRTSYGQNVLQHSIEVAFLCQVMADELGLNGALARRCGLLHDIGKAVDHEVEGGHPQ
                     IGADICKRFGERPEVINAIVGHHGDVEAISPYTALVAAADAISASRPGSRRESLERYI
                     KRLEQLEGIANSFPGVKQSYAVQAGREIRVIVDAANVDDSMASKTAYDIAKRIEQEMT
                     YPGEVKVTLIREMRAVEFAR"
     gene            18183..18395
                     /locus_tag="P5316_03250"
     CDS             18183..18395
                     /locus_tag="P5316_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84003.1"
                     /translation="MEDDVVRLLTEIRDNQREQLALAREQREIGNRAIDESVRLQRLA
                     IRRQMIGIIIAIVFLAIGLAAIFFKA"
     gene            18487..19836
                     /locus_tag="P5316_03255"
     CDS             18487..19836
                     /locus_tag="P5316_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR84004.1"
                     /translation="MRTVQRSLWSTAVLQVAFFAFSGAVVAADRPPNFVIIFADDLGY
                     GDLGCYGHPTIRTPNLDRMAAEGMRFTQFYSAGEVCTPSRAALMTGRLPIRSGMCSDN
                     PRVLFPSSAGGIPDGEVTLGEALKARGYATMCVGKWHLGHLPQYLPTKHGFDGYFGIP
                     YSNDMKPTPLMRNEQTVEEPAVQETLTPRYTEEAVRFIKANRDKPFFLYFAHTFPHVP
                     LHASARYKGTSPRGLYGDVVEELDWSVGHVLQTLRELGLAQNTLVFFTSDNGPWLTQR
                     QNGGSAGLLRDGKGSTWEGGMREPGIAWWPGRIKAGQVTTALAGTMDLFPTFVRLAGG
                     EIPKDRVLDGVDMGPVLFGTGPGNRNIHFYYRGQRLMAVRKGPWKAHLITQDGYGKNI
                     ETHDPPLLFQLEQDPSERFDVAKEHPDVVADLLAEIERHKATVQPVKCQLTDRVPGE"
     gene            20054..21628
                     /locus_tag="P5316_03260"
     CDS             20054..21628
                     /locus_tag="P5316_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR84005.1"
                     /translation="MNAIRTTCVRGVCCWLAVFVVAAVVHRGGRPAFSQSTAGPSQTT
                     APPNVLVVFDDQLRADACGVYGGKNTTTPNIDRLASQGVTFINAVSTCPLCTPYRGML
                     QTGRYPTHSGLVLNWVEANPRQRCIAHVFRDAGYRTGFIGKWHLASGRLKKAGKHAVT
                     PEDGRRIQRMQAEYQKENPETEYVPPGPARLGYDHWQAYNFHTTFNNAFYYEDSPQRL
                     IMKGYETDAEMDMAIEFMRRQQEKRQPFFLMVAPHPPHPPWKPQDCPAGYLEQIRQDL
                     IWAPNVPEDHALRKNPLPARCYYAMVKNFDDAFGRVLDFLDKSGLAQNTIVVLTSDHG
                     DMLGSHGRQNKMVPYREAVNVPCIIRWPGRIPAGKRIDVLLTPMDHFPTLCGLAGLQA
                     PTTCDGTDLSGALMGNRRIDRDAVLMANYVSHWDFFDTGTNWPEWRGIRTERYTYVKW
                     LDGKEELYDNVEDPYQMKNLAESPKDLPLLHALRIRLAELLADAHDEFLPGTAYADWY
                     DDERNLVKTALGPVER"
     gene            complement(22185..23990)
                     /gene="lepA"
                     /locus_tag="P5316_03265"
     CDS             complement(22185..23990)
                     /gene="lepA"
                     /locus_tag="P5316_03265"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRR84006.1"
                     /translation="MFDRNLIRNFSIIAHVDHGKSTLADRILVATKAVPAREMRAQFL
                     DDMEIERERGITIKANRATVFHEHEGQEYMLNLIDTPGHVDFHYEVSRALAACEGVLL
                     LVDAGQGVQAQTVANAYLAVAAGLDIIPVVNKIDLPGAMPEDVALEIEHVLGLPAEHA
                     VFTSAKTGFGIDELFRAIIERIRPPAGSIDVPLRALVYDAKNDVHRGVVCHVRVMDGS
                     IKRGDKIVLMAAGRGYTVSEIGKFMPKPQAVDSIAAGEVGYLIAGIKTIHDVNIGDTI
                     TLESNRAATALPGYQPPQRMVFCDFYPGPMTEAPQLREALETLWLNDSSFSFEPVHSN
                     ALGFGFRCGFLGLLHMNIIQERLEREGGVDVVQTAPTVTYEILKKSGEVIHIDSPSEL
                     PDMSVVEEIREPMITANLIVPADSIGSIMKIAEDRRGKYLKTEYLSPTRVMLTYEFPL
                     AETIFDFYDRLKAATKGYGTMDYELHGYHADDLVKMDILVNGTPVDALSVIVHREKAE
                     RRGRALIKRLRKEIARHLFDIPLQAAIGGRIIARETIKALRKDVTAKCYGGDVTRKRK
                     LLEKQKEGKKRMKMVGQVTIPQEAFMAILDPGDDG"
     gene            24170..26617
                     /locus_tag="P5316_03270"
     CDS             24170..26617
                     /locus_tag="P5316_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRR84007.1"
                     /translation="MQTYEKLGAFYLGRTYDLQQRRPTTDLVLYDSRDLVTHAVVVGM
                     TGSGKTGLCVTLLEEAAIDGIPAIVIDPKGDLTNLLLTFPELRPQDFRPWINEEDARR
                     QGLDPDSYAAKQAELWQRGLADWDQDGSRIQRLRDSAEFVVYTPGSDAGLPVAVLKSF
                     AAPPPEVVGDSEAMRERVAGVTAGLLGLLGIEADPVQSREHVLLSTILNNAWNQGQGL
                     DMPALIAAIQNPPFSRLGVMELESFYPAADRFALAVRLNNLIASPDFSRWLEGEPLNV
                     GEMLYTAAAKPRVAIFSIAHLNDAERMFFVSLLLSATLGWVRSQSGTASLRALLYMDE
                     IAGYFPPVSNPPSKAPLLTLMKQARAFGLGVVLATQNPVDLDYKGLANAGTWFIGRLL
                     TQRDKDRVLDGLEGAAAGASSEFDRAAIDKMLSQLENRVFLMNNVHEDTPAVIHTRWA
                     MSYLRGPLTRRQIRMLMDPYRRIVSKEAPVRMPQREGALPAEAVPSPTVRVPAERTSR
                     PILPPGVRQAFIPARSVPAGPNAVYRPMLLATATLRYADTKLSIDQEQRVVYLVPLIP
                     RSGSPVEWDSAQTTDLGEADLDPDPAATASFAPLPPEAANERSYQAWKKDFADMLYRR
                     SVLELLRCPSLEIVSRPGESEREFQLRISQAARERRDDEVTRLREKYASRMNVLQERL
                     RRARQAVEVQEEQSRASKLSTAVSFGGAILSALFGRKTAGVLNIGRAGSAVRGVGRSM
                     KEAGDVRRAEESVEAVQQKIADLEAQLRADIDAISQQTGEALEHIAVRPKKSDISVRS
                     VLLVWEPCWDTRSQVGG"
     gene            complement(26950..27198)
                     /locus_tag="P5316_03275"
     CDS             complement(26950..27198)
                     /locus_tag="P5316_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84008.1"
                     /translation="MRVREFFAFEAADLGFLWKHQLMLGILLIAIGLCVILFPGLLEL
                     LVATTAAVVGISMIGSALRFRRLYRRHRDFSLIDEFEW"
     gene            28291..29547
                     /locus_tag="P5316_03280"
     CDS             28291..29547
                     /locus_tag="P5316_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR84009.1"
                     /translation="MQHTAQAPPRIQYLRVQNYRALHDLELRNLTPLTVFVGPNGSGK
                     STIFDVFAFLSECFSVGLRKAWDKRGRFKELRTRGCEGPITIEVKYKEVNLPIITYHL
                     DIAEDSKGPYVAKEWLRWTRKVGKAGRPFNFLDFSEGQGTVISGELPEETDERIDEKL
                     DSRELLAVNTLGQFARHPRVGALRRFITDWYLSYLTADNTRGQPEAGPQERLSAWGDN
                     LPNVIQYLKEQHPERLDHILQVLSRRVPRLEKVDAEIMADGRLLLQIKDAPFERPVMA
                     KFASDGTLKMLAYLVVLYDPSPPRLVGIEEPENHLHPRLLPELAEECRQASQATQLLI
                     TTHSPFFVNGLRPQETWIMYRDEKGYTKAKQASEMEGITEFIEEGAQLGYLWMQGHFR
                     VGDPLASKPSSPPAQAKIPHRKTERP"
     gene            29562..30176
                     /locus_tag="P5316_03285"
     CDS             29562..30176
                     /locus_tag="P5316_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017293522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HRR84010.1"
                     /translation="MHIEFLVEELSAKAALDELLPRLLGTQATFDVHAHQGKHDLLAC
                     LPSKIRAYAGFLPADWRVVVLLDLDREDCKKLKRKLDRIVGDAGLRTKMSSGGQAGFQ
                     VLNRIAIEELEAWFFGDVAALHAAYPRVPLTLGQKSGFRDPDRIKGGTWERLEQVLQR
                     HGYFKSGYPKVTAAREIAKHMDPSRNRSRSFQVFRDGLRALLES"
     gene            complement(30248..32635)
                     /gene="cysS"
                     /locus_tag="P5316_03290"
     CDS             complement(30248..32635)
                     /gene="cysS"
                     /locus_tag="P5316_03290"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRR84011.1"
                     /translation="MTAKNDSKMESTSPGKRNLPDRPGLLSLIGNTPLVPIRRLNPNP
                     RVELYAKLERANPGGSVKDRIALYMIEEAERSGQLTRDKTILEPTSGNTGIGLAMVAA
                     IKGYSILLAMSEGVSVERRKILAALGAEFLLTPADQGTDGAIEKAYELAAREPDRFFL
                     PDQYNNRANLLAHYETTAPEIWAQTQERITHFVASMGTTGTVVGCSKRLKELNPAVRV
                     IGVEPYLGHRIQGLKNLKEAYVPGIYDRSAIDEKINIEDDAAYEMARRLAREEGLLVG
                     MSSGAAMFVACQIAESIDSGVVVAILPDGGERYLSTTLFQVAEPEVAPVKVHFFNSLT
                     GKYEAFEPISSGAKVTMYSCGPTVHARPHLGVLRRMLAADLVRRTLELAGYDVEHVVG
                     ITDLDDNTILAAESTGRAMSELCAQHEAEFHEDLRALGIKPASAYARASESVDDMIDL
                     TRSLVEKGFAYEKNHSVYFNISRVESYGELSGKDLGKIRIGATVDLERYDKNDPRDFT
                     LMRRCTLAEMRKGLSHRTEWGNVRPSWHVQCAAMARGKLGDRFDIHTAATDLVFPHNE
                     NELAESRALTGDSPARFWLHSELVLAKGKKMAYGQDNCITLPDLLEKGYSAREIRFFL
                     LQTHYRQPIELTDEGLDAAAASLRRLDECVAKLAAVNTSGPRVPELGPSILAMRESLR
                     EALFDDVNVSVALAALFRLVRQANYLMTQGRLFQEDAKEVMAALWAVDAVLGILLSAE
                     PPAHISPEIEDLVRQRDQARKKGDFATADRIRDVLTASGYLVEDSPSGTRVRRQS"
     gene            complement(32849..>34320)
                     /locus_tag="P5316_03295"
     CDS             complement(32849..>34320)
                     /locus_tag="P5316_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84012.1"
                     /translation="FTITAWRSVRTHGAAGSLGIALDPDAAPNEAVIEPRQGDVQIIE
                     VDLAQPAYPWTDLGFVYVTGSDASQPWPMSYDFINNDQTFRLFFDATDPDNRLNDQVC
                     YTIDLSNAIVDADLTTEILCDADCAIRMLAADADGNAIVEQADVSAIAAANGYTADAS
                     NVRLDVNVDGVINATDESIASSLLNNTVACPGGMMMMGMGGGVGLAGGADALVGTSDA
                     SLAPESFLGPSGRGFPSSLPPNAERIPRTEAARKGFRAKQDLLTLLRQQQVATPSANV
                     SAQWVVHGTGATSVTLPATGGTISVDLVVITDAPIWGFEGKPAVDAANVVSIDSADWT
                     ELDNVLFSHDVDGHAQSSHYDLTVMDWLWVRLTPDGSDVTAWGMDVDSLLGVDPPTVW
                     ALTQDLTAVNGPIAAPTAEPPIESQESAQWFTTTAGIGGVTREPLPAGQTVLATLSLT
                     ISPEPGTYHLTLTDAQFVDTIHVTHPATTGQPLEIVVGQ"
BASE COUNT         6941 a        10539 c        10418 g         6422 t
ORIGIN      
        1 actgggccag ctcctccagc acgcgacgct ggtcggctgt cggctcctgc agaatcgtgt
       61 gcaaggtggc gatgaccggt atgcgcagct cgcggagcag ccgcagcacg tgcgagcccg
      121 ccggcccgcc gaaaatgccg tattcgtgct gaaggatgaa cacgttggct tgattgccca
      181 tcacgtagtc ggccgccaaa cggtagtcgg gctgatagtt ggcgcgaagc tgaaatcgga
      241 cccgctccgg gtaacgatag ccttgcggaa tgtcgtcgag tgccagagca aaaacctcgc
      301 ccttgttgcc caactccgcg gcgatggcct cggccagatc actcgtgaac gtggcaatcc
      361 cgcatcggcg cggaacgtac gttcccatga tcgcaagttt cttcagatcc aatgcaggaa
      421 tcgtagccat gatgtgctca gaacctcctg atacccgcgg cgcacgaacc aaactacacc
      481 gaatggtcag ccaccccctc caacccccgg aaggtgacgg cgtttcgcca ctgcgttgcg
      541 tccccacttc aatatcgggg caatcgcccc gcagtcttga cacacgcaag accctgaagc
      601 atcgtcagta ttctgaactg catcagtctc tctcaaagcg agcatgccat atggtgactt
      661 cccaaaggta tcctgatttg cccgacgcct ccttcattcc cagggcctac acgggtctct
      721 cccgacaaca agtgtcggag ttgcgacacc ggtatctctc accggcacta ctactgtatt
      781 acagcaatcc aatcatgatt gtcgagggtc ggatgcagta cgtgtgggac gacacgggca
      841 agcggtatct cgacgcattt gggggtattg cggccgtgtc catcgggcac tgccaccctc
      901 acgtagccaa ggccgtccag gaacaggtga acctcatcca gcatacgacg gtgatttatc
      961 tccacccgac gatcgcgcgg ttcgccgaga agcttgcggc aacgttcccg gcgggtagcg
     1021 gcctgcaggt cagctatttc accaactcgg gaagcgagag taacgacctg gccctgctta
     1081 tgtgtcgcct gtacaccggt cgttttgatg ccattgccct gcgaaacgga tatcacggcg
     1141 gtactcacgc caccatggcc ctgaccgccc agagcacctg gaagtttccc ataccgcact
     1201 cgttcggcgt ccattttggc atgccgggct actgctaccg ctgcccgatg ggattgcact
     1261 atccggcgtg tgatttgaag tgcgctcgcg acgtggccca gacgatcctc tatgagacgc
     1321 cgggcgagat cgcgtgcttc atctgcgagg ccatccaggg cgtcggcggc gtaatcgtgc
     1381 ccccgccgga ttatctcaga atcatctacg aagctgtccg caaggccggt ggcttatgca
     1441 tcgcggacga ggttcaagcg gggctcggac gcaccggtga gacgttctgg gccttcgaga
     1501 attggggagt ggtgcccgac atggtcacca tggccaaggg cattggcaac ggcgcggccc
     1561 tgggcgccgt ggttactcgg atggatgtgg ccaaggggct ggcagggcgc ctccacttca
     1621 acaccttcgg cggcaatccg gtcgcagcgg ccgccggtct ggcaaccatc gaagcgatcg
     1681 aaacagagaa cctgcgcgag aatgcccgac aggtcggcgc gtatctcaag agcagattgc
     1741 tcgagatgca gcagcgacat ccgctcatcg gtgagattcg gggcatgggg ttgatgctcg
     1801 gcatggaatt cgtccgcgat cgtgaaagcc gcgagccggc caaggacgaa gcccatgacg
     1861 tggccgaact ggccaaggaa catgggctgc ttgtgggaac ctcgggcatc ttcggcaaca
     1921 ccattcggct taccccgcct ctgtgcttca cgaaggacga cgccgatttc gcggccgatt
     1981 gcctcgacca ggcgctcgcc gaggtccagg cccgtgcccg ataacggccc caagcggcag
     2041 tggccgctgt ctcgggtccc ctgggcattt caatgccgct ccgcccgcaa ctgtaagtcg
     2101 ttgtcctgcg gcgtcttgcg gtccgtgtcc cagggctttc cggcagttgc cgacgaaaag
     2161 gaaacggcaa tttcctggcc gcgggggcgt tatgcccgta tgttgggaag atgatggatg
     2221 ctgtggtact ggatgacctg atccgcagag cccgtcagcg ggattcggcg gcgttcgaga
     2281 cgctggttga gatctacagc ccacgtctgt acggctactt ttatcggctc accggccggc
     2341 gagaggatgc agaagatctg ctgcaggagg ctttcgtccg cgtcgtgcga atgatcagcc
     2401 gatacgagca tgacaaccga ttcgacgcgt ggctctttcg gatcgcaacc aatctcgtgc
     2461 gcgaccgggt gcggcggcaa cgccgatccg cggatgccgg ggcttcgggt gcccggcagg
     2521 atgagccggg gattcttgag gaggttgccg acaacgaggt tgaccggccg gatgatgtct
     2581 tggagaccac cgagcaggtt gaccggttgc agtgggccct gaagcagcta cccgaagccg
     2641 agcgagaagt gatcctgctg cgtcactttt cgcaaatgtc gttcagggaa gtggccgagg
     2701 caatggggac tccgttgggg acggcattgg ccagagcgca tcggggcttg gcgaaactgc
     2761 ggcggttgat gaacgaagac gccgcgtgaa gcaacaagag gtgcaatctc aggccgggaa
     2821 aagcaccgca aagccgcaag acggatgaga gaaatcgacc aaggatttat tgttcggcag
     2881 gaacagtgat gagtgacgaa ctgaacgaac tggaacgaga agttgagcag gaaatggcct
     2941 tgttgccaag cctcctggtg gagccgtcgg atgccaccgt gcttcgcgtg aaagccgtgg
     3001 tccgccacga gctcaacgag gaatggcttg ccggccaagc ggcgccgttg ccgtcggccg
     3061 aggccgttcg acgggtacat caggctgtta tcgccgaact agccaagccc gcctccgctc
     3121 gattgtcggc atggggcaaa acggcggcag tcttggccgc cgcggcaatg atcgcgatta
     3181 gcgtgggtgt gatccgacgg accggactgg ttggctctcc atctgtgtca cccgaggtgg
     3241 ctgaggccga gaaacacgtg gaacttttcc tggcaacggc cgagaaagtg ttctcgctcg
     3301 acgacttgac cgaatcaatt gccgatgacc tcagcgcgat cgaagaggac atcgacggtt
     3361 ggcaatcagc gggcagcggc gagaccggcg gacttggaga cgttctgcgt gagatcgacg
     3421 gaacaacgcc ggagcctctc gacaaccacg gcgtctctca tctctcgacc gtttctcagg
     3481 gagcctgggg atgaaatggg caacggcagg attactgacg gcggtcctcg gcggtctcct
     3541 cggcttgttc ggaccggcgg acagcaatga gtcccaaagc ccgaaaccgc gcgaccgagg
     3601 tgcttcagcg atcggccgcc ccgccgagac aagtgacggg ctggaaatcg agcagggtgt
     3661 tcgttctccc gcatggcctc gagacgcgtg gccgagacgc gattggcgag gtcgcgatgg
     3721 aacggtcggc ccgcccgacc gctcccgcta caggcaaggt atggggggac gctcctggga
     3781 cgatgaagat gacgaacaga tgtcgcccga gcaggtagct gagttgatga acttcgcgga
     3841 aagggagttt cccaaactgc accggcgact tgtaagcata cgcgagacgg accccacgtt
     3901 gttcagaaaa atgatcagac gcgtacgcgg tccggtctct gaaatcgtcc ggatccagca
     3961 acatgatccc aaggcagcca gacgattaat cgatgctcac cgcatcgaga tcgacctgac
     4021 ggagctgcaa agtcaatacc aggccgccca aaacgacgcg cagcgcgaac aactgaaggc
     4081 cacgatgcgg gaactggtgg tcaagcgctg cgagcttcgc cttcaaaggc tcaaagccga
     4141 gatcacagaa ttggaaaagc ggctcgaaca cgctaagaag gaaatggcca accgcgaaag
     4201 agaccaggat cagattgtcg atcaggagtt aagcagactc ttgacgaccg gaaccgcatc
     4261 gcccggcggg ggtcgtttgt ggccttcagg tgaactgggc cccggcttca ggggcggcaa
     4321 acgtggcccc ggtccgcctt cgacagggcc gtcgggcacg gtggcaccga tcgaatagga
     4381 gccgtctgcg tcgagcacga tggccttgtc gggcacaccg gcgctgaccg ccatattacg
     4441 catggcctcg gtctcgtggt tgggtcggtt gcccggcctg ccggacatga tcacggtgtg
     4501 ggtcagaccg ttgaggtagt aacggcacgc ggttcgcacg cggtcggcga acgcatcgga
     4561 gagtgtgcca tccgtacagg tccgggctcc aaaaacgacg accgcgtcgg cttgtcggcg
     4621 atggtcggtc ttgccgaagc agtctgggcc ggggcggaaa ggcggagtgg taacaggacc
     4681 gcagcgccaa cgaggcacat gtttccgaca acggggaaca ggaagccaaa gccgatccac
     4741 cggatgccgg gatcatgacc tggccgaggc ggattgccga gcaggttcag tagtgccccc
     4801 ccaacggatg ccgccgggcc gcaaacaagg ccgtgtcaga ttaaagaaca ggtgtcgcca
     4861 tgagtcatgc cgttttctgc agtctctgtt tgagccttct gacttctccc ttgtacggtg
     4921 tatcttctcc cagcatcgtg cttctctatg ctgatgacct gggctacggc gacgtcgggt
     4981 gttacggcgc gacgcggatc aaaacgccga acatcgaccg cctcgctcaa cagggcttgc
     5041 gattcaccga tgcccactca tcggccgcca cgtgtacgcc ctctcgctac tctctgctga
     5101 ccggtgaata tgcctggcga cgaaaaggca cgggagtcct gcccggcaat gcccggctga
     5161 tcatcgagcc cggccgaacc acgcttgcct cgattctcaa gaaagccggc tacacgaccg
     5221 gcgcggtcgg caaatggcac ttggggttgg gagacgacaa gctcgactgg aacggcgaga
     5281 tcaagcccgg cccgctcgag atcgggttcg actactgctt cctgattccc gcaacgggcg
     5341 accgcgtgcc ctgcgtctac gtcgagaacc accgcgttgt cgggcttgac cctgccgatc
     5401 ccatcaaagt cagctatgac gagccgatcg gcgacgagcc caccggcaaa gagcatcctg
     5461 agttgctcaa actgcatccc agccacgggc atgaccagac catcgtcaac ggcatcagcc
     5521 ggatcggcta catgagcggc ggcaaggcag cccgttgggt ggacgaggac atggctgacg
     5581 tgatcaccga caaagccgtc gcgttcatcg agcgaaaccg cgacagacca ttctttctgt
     5641 acttcgcgac gcacgacgtg cacgtgcccc gcgtccctca tccacgattc gtcggtaaga
     5701 gcgagatggg cccgcgcggc gacgtcatcg tacagttcga cgcctgtgtc ggccgtgttt
     5761 tggatgccct cgatcgcctg aaactgaccg aaaacaccct ggtaatcttg agcagcgaca
     5821 acggcccggt ggtggacgac ggctaccggg accaggccgt cgagaagctc ggagaccaca
     5881 agcccgccgg tccctggcga ggcggaaaat acagcaactt cgagggtggc acacgcgtgc
     5941 ccttcttggt ccgctggccc gaccgcgtga aacccggcac gagcgaggcc ctgatctgcc
     6001 aggtggatct cattgcgtcg tttgcggcac tcaccggcca gtcgctcggt cgggatgacg
     6061 cacccgacgg catgaacgtc ctgcccgcgt tgttgggaga atccaaaatc ggccgcgatc
     6121 atctggtgca gcaggccggc gcgctgtcgc tgcgaaaagg cacgatgaaa tacatcgcgc
     6181 caagcaaagg gcccaaggtc agtcgttaca cgaatgtcga actgggcaat gatcccgcgg
     6241 cgcagctcta caacctggcc gacgaccccg gcgaaacacg gaatctggct cccgacaagc
     6301 agcaagagac gcaagaactt ggcatgtcgc tgcaaaggct ccaggactcg ggccgatcgc
     6361 gaccctgacc ggcgaaccgg atcgcgagac aggggccgcg ctcgctgtgc ggggcctttt
     6421 gctgcttcga gatcggggtt acgcgcttgg cggcgcgacc ggaatcggct ccggcaccgg
     6481 cgcgacagcc atgcctttgc gccggtcacg ctcgacacgg aacatgatca ccgaacccaa
     6541 agcgaaaagc acgaccgcca gcgcgagcgg catgcgaaga tcgcccgtcc aacggtagag
     6601 agccacggcc gggaactggc ctatcagccc gcggatacca accatcgtcg cgtgaatcgc
     6661 caggtaggtc ggagcttgcg aggcgtcccg ggccaggaat atcggcccga gcgtccatgc
     6721 caggttcacg ccgctgagca tcacgccgta caggatggtc acgatcatca ggctgttgac
     6781 gtcgtacgcc cacatgaggc cgatcggata gagcgctaac gccgcaaagg agtaggccgc
     6841 cgtcttgagc ggcccgagct gatccatcag ataggccgtc ggcagcatcg tcagcaacag
     6901 tgtcaactgc aggaccgtct gggtggaccg ggcaacctcc tcgtacgtca gcttcaactt
     6961 gtcaacgcac aaaaacggca gcagggccca gcagatcatc cagcccagcc cgtaaatcga
     7021 gaaggccgtc tcatgccgcc ggaaaatgct gtcctctcga aagactctca gcatattgtg
     7081 gtacactcgc cggaggctgg tgcgcagccg ctccgacggc ttggcctgct ggcgctcgat
     7141 gaacaattgc cggcgagtga tgcaatagag caaccacatc cccatgccga ccagcaccgt
     7201 cccgaccggc aggtaccatc tgaacgaatc cggccgccag ttgagccaca gaccgataag
     7261 gtaagtgccc accatgaccg tgaactgcgt caccatctga acaacgctga agatccggtt
     7321 ccgcgacgcc ggcggataac agcttcgcag gatgtcgccg gtgagcggct gcatgccgcc
     7381 gaagccggtt gcggcgatca cgatgaatac gagtgacgtc catgccttgt gacataacgc
     7441 gatccccgcc agaggcgcga tggctgaaat ccatagcagg gccagatacc gccctgaccc
     7501 aatccggcgg tacagctcgt tccagacgac cgacagcacc agggtgatcg aaatggcgga
     7561 cgtcgagatg accgtttccc attcgctggc ctggaaataa gaccgcagca gaacgggtag
     7621 catctgaaac accgccgcct gcagggcaat gaacggcgtc gcccagagct ggagccgaag
     7681 gatgttgcgg ctttcgggaa atgcgaccag acgatcgaga atcaaacaat gcccccattc
     7741 aacacgcgac aacgaaggcc atagtctaat tgaagcggag ccctcatgga atcaccggat
     7801 cggtaccctc gctcaacggg tactcgtgca tgaggtggtg gaactcctcg ccggaccgca
     7861 cggatcgcat ccggtgtcgc cactcgggcg acggctggag cttggcggca taccaactga
     7921 aacgctgtcg catgatcgcg caggctactc gctcgctctt gatgcgcttg agctgctcga
     7981 aatgccggac gacaagatca agccgatcgc gcaccgtcgg cggcggcggc aagatgccgg
     8041 ttgtcagata ggcatgtgaa tcacggaaga tccacggatc gctcaacgcc cctcggccga
     8101 tcatcacccc cgcacagccg gtagcgtcaa tcatccgctt ggcgtcggcg gggctgcgga
     8161 cgtctccgtt gccgatgacc gggatggatc gcaccgcctc gaccacccgg gcgatgccgt
     8221 ccagccggac cttcccgccg aacttgtccg ccgccgtccg cccgtggacg gtcaccgccg
     8281 ccacccccgc ccgttccaag tcggccgcca gttgcggtgc caccatcgac gaatcatcca
     8341 aacccagtcg cagcttggcg gtcaccggca aatccaccgc ccggacgacc gccgccgcca
     8401 gccggaccgc gctggctggg tcggacaaca tcgctgcccc gccaccacgg gtgcagatct
     8461 tggccaccgg acaacccatg ttgatgtcga tgacgtcggc tccatggtcc cggcagtatt
     8521 tggctgcgtc gatcatctcg gccgggttgg ccccgtagaa ctgcacgcag agcggcttgt
     8581 catccggctc cgtgcggatc aaggccatcg atgccccggt ttgccgcaat accccccgcg
     8641 ggttgaccag atcggtgcac gccaacccga gcccgcccaa cggccggacc accagccgga
     8701 acgccagatc gcagtatccg gcgatcggcg acaacaacag gttggagatc agtctgagac
     8761 tcccgagcga gaacactttc tcggcctttc acagtcaaac cgaacgcgtt tcatcgggcc
     8821 gcatcttcgc cgaggtgcag cctcccgaca actcccatct tgcgatcgac caaccttccg
     8881 cgagcatgct tgcacaaccg tcccgacacg cctgataatg cagagggctt tgaaagcggt
     8941 cagacggcag accgtgaggc cctctgcccc tcgactcaaa agaagtttgc cagcggacaa
     9001 ggccgcgaga cgcacacgta aacgatgtga aagctggctg acccgaaaac gagcccgcat
     9061 accgctcgtc tccggatggt cgcctgaaaa ggactgccgc gaggtaagac aatcgctctg
     9121 gattcaccca tttgttgagg agctgccgcc tatgagacac ctgcaactct ggatggcact
     9181 tgtggtcaca tggagtctct ctcacgcggt atccgcaggg gccggcgcac ggtatcggat
     9241 gctcagctca gtagcgcgaa ggttgcctcc agtgatcgaa tgccgtatca ccgtcggtcc
     9301 cactgaaacg acgcccgacg gaacccaggg ccagtggtgg tccattcgtt tccagaaaga
     9361 ggacgaggat tttcagatct ggtgcctttc cgagcaggtt ccgatgcttg ccgcgttgcc
     9421 cgccacaagt cccggccgag tgtggcgata catctatcgc gagggtcaca ggccgatact
     9481 cgaataccgc cgagaaagca ctggggaggt cctgcttccg ggatttggct ggcaggatca
     9541 tttcctcccg cacccgaagc gtgccaccga acgcagctca ggctggctgg tgagcgggcg
     9601 gtgcatgggg catgtcatcg gtttgttgga gacgcaggaa tcagccgagt gggaccaacc
     9661 ctccccccag gaagtcacca tgctgccgct ggacgatgga ctgcttgttg ccacctgcct
     9721 ttccaagcga gacatcaact acggcaagaa gccggacggc gactacacct accgacgctg
     9781 ggatgaggcc gatgtgaagg cggttatcga agccggcttc aacatgatga tcgtgcacag
     9841 ccctgaactg gaagactggg tgtggaagca accggtgttt cagttgggtg ctcctgaccc
     9901 ttacccccag atgtttttcc gaagcgccta cctcggatca catttcttca ttgatgaacc
     9961 cgcgcaccac gtggcgactc gcgagcaacg ccgcttcttc gccgatatcc cgcgttacga
    10021 cctcatgtac gaccttctgg cctctttcac tgaagtcgcc ctcaaggagc aatcctcgca
    10081 catcatgaga cctggatcgg cctgtcttat gggtaaagcc gtgccggacg tcaactgggg
    10141 tactctggag gtgcgaaatg agctcctgcc ggcatgggaa acccactgcc ccgccgcgtt
    10201 ctgcgaaatg gatgccggcg ccagaggcgt tgtcattgag ggccggtttc aacgccccgc
    10261 attcaacgcc cagattaccg agctacttgg tgaagacatg aatgtcaacg ccgaggaaat
    10321 gctggcgtgg catgtggcct tcctgcgagg agcagccaga gcattcaaag gggagtgggg
    10381 catgagcatc tatggccagg ccgaccccga gatcgccaag ctggccgtgg tgaacgccta
    10441 caagctcgga gcccgctaca tctggttctg gacgaacgat catgaccacc atgtcccgtt
    10501 cgaacgccaa ttggaacttg cccgcctggt caccgatgcc aagcgaaacg accgccaacc
    10561 acggaacctg gcaagactcc gctcggccgc aacggcagcg gtcgtgcttc ctacgggcta
    10621 catcttcaca atgggcggcc cactgcttgg caattactct ctgagcaccg acagcaagaa
    10681 tgctgcgacg gcaccgcgaa gggccgttca gcagaccatc gtgcgaaccg gcctggagct
    10741 gatgagaaac ggcgtgactt ttgactatgt caatcagtgt ccccatcttg cttcgctggg
    10801 ttatgatcga ctgctacaca ttcagcccga tgcgacgttg cacgagacga aaaacggcag
    10861 tttgccgtaa gccgtttttc tgggatagcg ggcaaaagcg gatacggcca gttttgcggt
    10921 gccaggcgtg cgggcgcggg tgtcggaacg aggacgttgt ttttggcacc gatggactta
    10981 cggagccagt gcgttcaaac aagcgcgctt ccttgttcgt attcgcgtac cgctctctac
    11041 accatcccaa cacagcgatc tcctcataag taattggaga cacagggttt gcgcctcctg
    11101 tcgcgcatgc tgtctcgaag tcacgttgcc tgaaatcaac acccctgcac attccggtcg
    11161 aaggcgagct gaagccctga atcgtcgcaa tctatgtaac tacttgtaaa ccaataagtt
    11221 agtcctagct tgggccgcct gtgatgatcc ggcactactg gcaccccctt tgcccacaga
    11281 actggcaaaa ccgcccgcca gccgtccgga ggtgggtggg accgtggacg caccggtgac
    11341 cagccggtgg caagcagccg acggggtgac ctgatggaag agggttcctt tcacaagacg
    11401 ctcagcaagc tggtcagtga gatcaagacg atgcccgagg tggaaaagag caaactggcg
    11461 aaactggcgg aagagaccaa gcaccggcac gagcagctca agaagacggt gagcagcctg
    11521 caggagagca tcgactttct tcggctgtca atcaagtacc ttctgttcga cctggaggcg
    11581 accaagcgcg aaaacgtgca gcttcgcaag atgctggagg agcgctccga ctcgtaagtc
    11641 ggattctgtc ggaatatccg ttgctcggcc cggaaccagc gttcaagatg ccgcccgcaa
    11701 tggaacctga gggccgatgc gagccatcgg ccaggccctt caagcccaag acctgcagcc
    11761 aagaggggag cgaaggagga caggcagggg gcaagaaacc ccgatcgttc ggggaaaagc
    11821 aaagaagtct tcgagacgcc gtaattgtgt tacgcttgaa gacgaaaacg gcgaacgcct
    11881 tccactcgct gcgttcgccg ttttcatgcg cccgtatcgg ttgatcggtc cgccgcgaga
    11941 ttctgccgcg aggcggccgt caaatgactc atcagaagaa gggtaacccg ataaagaagt
    12001 tgaacacgcg cgtgtcgtcc tcgctgtcct tgataaccgg ccaggcgaga tcgaactcca
    12061 tcgggacggt cccgaaaatc tccagggtaa gccggactcc acagcccact gatgcccgcc
    12121 aactgctgat gccgaagtcg cgctcgacgg tacccatgtc gctgaagaag acgccgcgaa
    12181 tgaccttgtc gtacatcggg aaagagtatt cggcacccgt caggagcatg aagtcgccgc
    12241 ccacgcgatc ctttttcaac cccgagcggg ggctgattcc acgaaaatca aagccgcgca
    12301 tggacccgat gccgccggca tagaagcgct cgaacaccgg cgcgtcgccg agaatctgtc
    12361 cgatgttggc cctggcactg accacgctct ttcggtcctg ctcatcgatg gccacggtcc
    12421 agtgctgcgc gtatccggcg gtaagtttgg aatgcgtgta atcgccgccc atgacgcccg
    12481 cctgttccca ggacaggttt gcccggtggc ccttgcccgg atcaagccgg ctgtcggtcg
    12541 tgtcatggac cagcgagaac ttgatggtgg acagataact gccgcctttg actttctgaa
    12601 tgtccctggc tgagaacagt tcgacgtcat cgacgttgac atactcgggc cgcagggcga
    12661 tctcgccgag ccaacccttg agcaggccct cctcgaactt cttgtcaaag ctgacataga
    12721 cgccggctcg ctgttcggtg tacgcgtctc gcggacgctc gaacaggaaa acgccggtat
    12781 tgaagccgat cggtctgtcg ttcagatagg gctcgcggaa atcgatgcga aaccgcgtgt
    12841 attcggtgcc gggctcgaat tgcacgcgca tcgtctggcc ggcgcctttg aaggcccgac
    12901 ccttgaacag ctcatcccag gttcgaggcg tgtcgaaaag atcaaagttg gtgttttcca
    12961 gcacgatgtt gccgaccagg ccgctgtcgc tgctggcccc gatgcccgcg atgaaattgg
    13021 tggttttcgt gttctcctcg acggtcaccc gcatgtcccg aacgccgggc tgcggcgcgg
    13081 acggttcgac ggcctcgacc gtcgcgccga tgaacagccc cgtcgccttc agatcctgct
    13141 ccgccttggt catcttggtg acgtcataga tgtcgcccgg gtacagcccc acctctcgtc
    13201 gaatgcactt ctcctgcgtc cgctcgttgc cgtcaacctc gatccagccg acggtgaact
    13261 gctcgccctc ccggatgctc agggtgagga taaccttggc cggctcagag gcaaaaaccc
    13321 agctcgccgt gaccttcacg tcgatgtatc cctgcgagcc atatttggtt tcaagccgtt
    13381 tcacatcgct cttgaggcgg gcgtcgatca aggggtcgcc cgtcttgaga tccatctcat
    13441 tgagcagctc ctcggcggtg aacacttggt tgccctgaat ccggatctcg gcgacggcgt
    13501 accgaacacc ctcgttgacg cggaagatga catcaagttt ctcacgggcc acatcttgga
    13561 actcggtcac gtagccgacc tcggcatcga ggaacccccg atcccgatag tatttctgca
    13621 gcgtggcggc atcccgcgat gcctgatccg cgtcgaagtt cccggtccgg aagatgggta
    13681 tgtaactctt ggtctcaatc tgcctcatca gttccccggc gtcgtaggtg acgttattct
    13741 cgaaacggac cttgcggatc ttgacccgct ggttctcaat caccgtgtag accacgcggc
    13801 gttcggcggc cagcaggttc tcgtcgtagg ttaccgagac atagggatag cccttctcgc
    13861 gatacagtcg ttcgatggtg tcacgaccct gccgcacctc gtgcatgtcg agcggatcgc
    13921 cggcggcaaa ggtcagctct ttgagcaagt ccttggtctt gattttctgg ttgccgacga
    13981 tttcgagggc ctcaacctcg ggcttctcca ccagtgagaa cgtcaggttg atttgaccgt
    14041 cgaccagcac cggctcggct ttgacgtcga gaaaccggcc ggtcctgagc aactgccccc
    14101 ggtcccgctc cacctgatcc tgagagtaag tctcgcccgc acgcgtgcgg atgcgattgc
    14161 ggacataggc ctcttcaatc cgcctcaagc cggtgatgtt gagttgtcgg atctgccggc
    14221 catccagcgg cgaagccggc cccgcaggca aagtggccgg cacctgggcg accagtggcg
    14281 gcgacgtgag cgggaagata acgagtcctc ccagaagcaa acataaccac cgccgccggt
    14341 cgttgcccaa ccgagtcgcg tgctgtcgtg tcattgtcgt gtgtccgtca gaagtcgttc
    14401 tgagacgcca gtgaccgcac cttggagtcg tatgatacgc ggagcaatgt ggctgtcaaa
    14461 aaaagaaccg acagcactcc gggccggcgt caagccttgc ggacgaaccc cctctcgggc
    14521 gccttcccga cagcccgctt accccggcaa gaccccgcaa gtcgcagcac ccgcggctca
    14581 ggaccgcaga gacctcaaat gtgccgtgaa ccacttgaca caagccttgg gtcttccgca
    14641 cgcagtgttg ccggccggaa accgccacga cctcccggtt acggcccaat cgatgtgagc
    14701 atcaccgccc cgactcctca attggggtag ccttgtcatg cagaccccgt gcgaagccgg
    14761 aaccttccga tcgcctgcgg aaatcaagag ctgctcacac catgcggata gatgtcaccc
    14821 gcacctccgg catgatggac acctgctgcc aacaaggcgt gggtcgtgaa gctcgtacgg
    14881 attcatgttg tgtgtcccgg ttgccggcag ccaacatgtt agaaccgggc gtcatccttg
    14941 ggcttcatgg cgacggtcac ctcgatctca cggccgatag ccatcaggtc gtcctgaaga
    15001 cgaccggcgt ccacggaggg gggtatctcc acttcgagca acatcacgta caggggctgg
    15061 gcgtgatgaa tgacctcggt gtggagattg gtgatgttga cgccgaactc ggccagccgc
    15121 ttgctgatcc ggtggataat gccctttcga tccgcaccat agacgtgaat caggtgcttg
    15181 gactgtgacg acctggcctc gcgcgcctct tcgggcctga gatcctgcag gtacatgcgc
    15241 aaaccgagct ccccggccac cccggacaag cgatcctcaa gttggcggct tgccatgccc
    15301 tccggcatgc gaatcatgag catgcacgca aagtcggagg ccaaacgagc catttgcgag
    15361 tcctcgatgt tgcagtcgct ttccaagagc accccggtga tggccgcgac gatcccaggg
    15421 cggtcgggtc cactggcagt cagcaaagcg tagttgggca ttcccaagca ccttccaaag
    15481 ccggtccggc atgacgaccc cgaccgtcga accaccgcag cggggccaag acgacggttc
    15541 gaccagtgta ggagcccaat tgggctattt caatccgcgg ccgacggtca ctcaggtcac
    15601 cgtaagctgg aggccggaac cgcagaccgg ccaagaaaat taaccgtgac ggtttttcgc
    15661 ctatgtgtaa cctttttggc tggaagaggt tgtacagggc atcatatccg ccgcacttga
    15721 cgatccttac agaaacctta taatgcgtgt tgacccctcg cgtcgagaaa ggcgcggaga
    15781 ggtattttca tacgggaact cgaccgattg actgccgttt gggaaagttc gacaacagcg
    15841 gcgggcgatc ggggagttcg ggtctaggtc atgcgaccgt atggtcgggc cttgatcgtc
    15901 gggagattca tataatccgg atggtacctt gcccaaggca aggtcaatcc tgaggttcac
    15961 acagatagtg gtatcggccg gtgctcgatc cgggttccgg cggatatcag agtggggact
    16021 gaacccacag cgcgttcatc ggtgttgacg gccagcgtgc cgaagaggtg gtgtcgaccg
    16081 caaaccaagg tcgcgcgaag tattaaggaa ggtgagtggc ttgagaaatc caggtggcac
    16141 cggtcaggcg acttgcggat gtcggacaga cgccgcgtgc agggcccacg gggcgcggag
    16201 tcccgaccag agccctcccg atcgcaccgg atagatgccc acagcgactt accacctgca
    16261 tgtgaccgaa ggcacgacac cacaaacctg cggtgctggc ggcgttcgaa cgattgtctg
    16321 cagaacctcg ccttgacttt gccgacggcg gtccccctgc cgaatcccag ggctcgtttc
    16381 cggtagctct cctgaccgta tctagatgat ttactttaat ccgctccgtg atcgggcggg
    16441 gatgtgctca atccgcgctc cttgccggcc ggcagcatag agaccgatca ggaaggtgta
    16501 gaatgggact gcctttcact ttggctgtgg cagattatgt tccggcgcta ttggtgatct
    16561 cttcgttgat taccggggcc gttgccactt acatgggcct gcggatcttc ggcaagcaga
    16621 aactcgccac cgcacaacgc gatgccgccc agatcatcga aaaagcgaaa aacgaagcgg
    16681 aggtgatcat caaacgggcg gaggtcgacg ccaaaggcga gttcatccag cgaatggatg
    16741 aattcgagaa gaagagcgcc gccatccgcg acgaattgaa ggaacaggag cgccggctgg
    16801 agaaacgggc tgacaacctg gataaaaagc tcgataccct ggtaaccaag gagcgagccc
    16861 tcgagcaggc cgagcaaaga ctgagcagtc gggaagcatc cctggcccag aaagaaaccc
    16921 aactgactga gattctggag caacagcgcg cccagttgct ccgaatcacc gggttgagca
    16981 tcgatgaggc ccgcaacctg cttctcaacc agatccggca cgaggtggaa cacgacgccg
    17041 ccgagatggt cgacaggatt ctgaccgagg ctcgtgagac ggccgagaat aagtcccgcg
    17101 agatcgtggt caccgcgatc cagcggtatg ccagtgagca caccagcgtc aacaccgtca
    17161 gcacggtgga tgtaccttcg gacgacatga agggccgcgt catcggccgt gagggccgca
    17221 atatccgggc gtttgaaaag gccaccggtg tggacgtcat cgtggacgac actcccggtg
    17281 tcgtggtggt cagcgcgttc gatccggttc gccgtgaggt ggcccgactg tcgatggaga
    17341 aactcattca tgacggacgc atccacccgt cgcggatcga agaagtcgtc gaggaaacgg
    17401 gagcggaggt ggcccgtgag atcgtcgaac gcggcaaaaa ggccgccgtg gaggccaacg
    17461 tcggcggcct gaatcgcaag atcatcgatc tgctcgggcg gctgagctat cgcacaagct
    17521 acgggcaaaa cgtccttcag cacagcatcg aggtagcgtt cctgtgccaa gtcatggccg
    17581 acgaactcgg tttgaacgga gctctggccc gccgatgtgg gctgctccat gacatcggca
    17641 aggccgtcga ccatgaggtg gagggcggcc atccccagat cggcgccgat atctgcaagc
    17701 gattcggcga gcggccggag gtgatcaacg ccatcgtcgg acatcatggc gacgttgaag
    17761 ccatcagccc gtacaccgcc ctggtggcgg ccgccgacgc gatcagtgcc tcgaggccgg
    17821 gcagccgccg cgagtcgttg gaacgttata tcaagcggct cgaacaactc gagggtatcg
    17881 ccaacagctt ccccggcgtg aagcaatcct atgccgtcca ggccggccgc gaaatccgcg
    17941 tgatcgttga tgccgccaac gtcgacgaca gcatggcttc caagacggcc tacgacatcg
    18001 ctaaacgcat cgaacaggag atgacctacc ccggcgaggt caaggtcacc ctgatccgcg
    18061 aaatgcgggc ggtcgagttc gcacgatagt cgtcagacgc cggatcgccg cggcaactgc
    18121 tggcgaggca gatgctcgtt ccgctcgttc tgaagttgtt ttgtcttctt ggggtgctgc
    18181 aaatggaaga cgacgttgtc agattgttga cggagatacg cgacaatcag cgggaacaac
    18241 tggccttggc cagagagcag cgggagatcg gcaaccgggc catcgacgag tcggtgcgct
    18301 tgcagcgtct cgccatccgc aggcagatga tcgggattat catcgcgatc gtctttcttg
    18361 cgatcggcct ggccgccatc tttttcaagg cataattggt agtaagcgtt gatcgagtcg
    18421 ttgggtcatt ctgcggactt gtggcgtccg gattcgggcg gccccgaaac caaggaggca
    18481 gctagaatgc gaacagttca gagatcgctg tggtcgactg cggttctcca agtcgccttc
    18541 tttgcattct caggtgcggt tgttgccgcc gatcgcccgc ccaatttcgt gatcatcttc
    18601 gccgacgatc tgggctacgg cgacctgggc tgctatggtc acccgacgat ccgcactccc
    18661 aatcttgatc ggatggccgc cgaggggatg cgtttcacgc agttctactc ggccggcgag
    18721 gtctgtacgc ccagccgtgc ggcgctgatg actggccggc tgccgatccg cagcgggatg
    18781 tgcagtgaca acccgcgggt gttgttcccc tcgtcggcag ggggcattcc cgacggcgag
    18841 gtcacgctgg gtgaagccct caaggcccgg ggctatgcga cgatgtgcgt cggcaagtgg
    18901 caccttggac acctgccgca gtacctgccc acaaagcacg gcttcgacgg ctacttcggc
    18961 attccctaca gcaacgacat gaaaccgacg ccgctgatgc ggaacgaaca aaccgtcgag
    19021 gagccggccg tccaggagac gctgacgccg cgatataccg aggaggccgt ccgcttcatc
    19081 aaagctaatc gcgacaagcc gttcttcctt tacttcgcgc atacgttccc gcacgtgcct
    19141 ttgcacgctt ccgcacgata caagggcacg agtccacggg gtctctatgg tgatgtggtc
    19201 gaggaactcg actggagcgt cggacacgtg ctgcagacat tgcgggagct cggcttggcc
    19261 cagaatacac tggtgttctt caccagcgac aacgggccat ggctgacgca acggcagaat
    19321 ggtggctcgg cggggctgct gcgggacggc aaaggaagca cttgggaagg cggtatgcgc
    19381 gagccgggca tcgcttggtg gccgggacga atcaaggccg gccaggtgac gacggcactg
    19441 gccggaacca tggacctgtt tccgacgttc gtccgactgg ccggcggcga gatccccaag
    19501 gaccgggtcc tcgacggcgt ggacatgggc ccggtattgt tcggaacggg cccaggcaat
    19561 cgcaatatcc atttctacta ccgcggccag agactcatgg ccgttcgcaa gggcccgtgg
    19621 aaagcccatc tgatcaccca ggacggctac gggaagaata tcgaaacgca cgatccgccg
    19681 ttgctcttcc agcttgagca ggatccatcg gagcggttcg atgtggccaa ggaacacccc
    19741 gatgtggtgg ccgatctgct ggccgagatc gaacggcaca aggcgactgt ccagccggtc
    19801 aagtgccagc tcaccgaccg ggttcctggc gaataagccc gcggtcggcc gatctttacg
    19861 gagccccgcc ggcagtatag ccttgcgtcc cggtggccgt tcatgcaagg cgggatgaag
    19921 gagccgctat ccgggtcagt ctgcaactcg ttggcccgca attctgttga ttgcccgact
    19981 cgcctgctcg cccggatccg ccgacgccgg cggcgcgtga cacccgccat gacaggagat
    20041 tccagtgatc gacatgaatg ccattcgaac aacttgcgtg cgaggcgttt gttgctggct
    20101 cgccgttttc gttgtggccg cagtcgtgca ccggggcggc agacccgcct tctcgcagtc
    20161 aaccgccggg ccttcgcaga cgaccgcgcc gcccaatgtg ctggtggttt tcgacgatca
    20221 gcttcgagcc gatgcctgcg gtgtctacgg ggggaagaac accaccaccc cgaatattga
    20281 ccggctggcc tcgcaaggcg tcaccttcat caatgccgtg tccacctgcc ccctctgcac
    20341 gccgtaccgg ggcatgctgc aaaccggccg ctaccccacg cacagcggcc tggtcctcaa
    20401 ttgggttgaa gccaaccctc gtcagcgctg cattgcccac gtgtttcggg acgcaggcta
    20461 ccggacaggc ttcatcggta agtggcacct ggcgtccggc agactgaaga aagcaggcaa
    20521 gcacgccgtc actcccgaag atggccggcg gatccagagg atgcaggccg aataccagaa
    20581 ggaaaacccg gagaccgaat atgtcccgcc cggcccggcc cggctgggat acgaccactg
    20641 gcaagcgtac aacttccaca cgacgttcaa taatgccttc tactatgaag actcgccgca
    20701 gcggctgatc atgaagggat atgagactga tgccgagatg gacatggcga tcgagttcat
    20761 gcgaaggcag caggaaaagc gacagccgtt ctttctgatg gtcgcgccgc atccgcctca
    20821 cccgccgtgg aagccccagg actgcccggc gggttacctg gagcagattc ggcaggacct
    20881 gatctgggcc cccaacgtgc ctgaggacca cgcccttcgc aagaatcccc tgcccgctcg
    20941 ttgctactac gcgatggtca agaactttga tgatgccttc gggcgggtgc tcgattttct
    21001 ggacaagtcg ggcctggccc agaacaccat cgtggtgttg acgtccgatc atggcgacat
    21061 gctgggcagc cacggccgcc agaacaagat ggtgccctac cgtgaggcag tgaacgtccc
    21121 gtgcatcatc cgctggcccg gtcgcatacc tgccggaaag cggatcgacg ttctattgac
    21181 gccgatggac catttcccga cgttgtgcgg gcttgccggc ctgcaagcac caacgacctg
    21241 cgacggcacg gatctgagcg gcgcgcttat gggcaatagg cgaatcgacc gcgacgcggt
    21301 ccttatggcc aactacgtgt cgcattggga tttcttcgac accggcacga actggcccga
    21361 atggcgtggc attcggaccg agcgatacac gtacgtcaaa tggctagacg gcaaggaaga
    21421 actctacgac aacgtcgagg acccctacca gatgaaaaac ctggcggaga gcccaaagga
    21481 cttgcccctt ctccacgcgt tgcgcatccg cctggcggaa ctgctggccg atgcccacga
    21541 tgaattcctg cccggcaccg cttacgccga ctggtacgac gatgagcgga acctcgtgaa
    21601 gaccgcgctg ggacccgtcg agcgatagtc gcaggggctg tcggatcggt cggctgtctt
    21661 tttttgaatc ggcggcggcg tgaaaggccg gctgagggtg tccgactcta ccggccaggg
    21721 gtcagtacat acgtgcggtg attgagagct ttcgcgctca ccttaccgat tcatcgccgg
    21781 ggtcacggat gaaggtgatc ttgcgttggg cgggctccga cgtcaccccg tgcagtgtcg
    21841 gataccgaag accctttgcc acagggtgaa ctgatacccg cattttcggc aagacttggg
    21901 catgaaacct cgggccggat ggccacaatt ggggcaaaac atcgaggggg ttagggcgaa
    21961 actctgtgct tcgaggtgag gaatctgccc tggtttcgcg ataacaaaag cagcgccgca
    22021 ttccgggcaa cgagcagagg tcaaagcggt aagattgtag ccgcaagtcg ggcagcggag
    22081 cccggtgtca tctgcagcgc ggtcgatttc gggcggttcg tcgggcaagt cttgccttcc
    22141 cttcgtgcgg caggacatgg ccactccgtc tcttgctcaa tagattaacc gtcatccccc
    22201 ggatcaagga tggccatgaa ggcctcctgg gggatggtga cctggccgac cattttcatg
    22261 cgcttcttgc cctctttctg cttttcgagc agcttgcgtt tccgggtgac gtccccgccg
    22321 tagcatttgg cggtcacgtc cttgcgaaga gccttgatgg tctcgcgggc gatgatccgg
    22381 ccgccaatag ccgcctgaag cggaatgtcg aacaggtgcc gggcgatctc cttgcgaagc
    22441 cgcttgatca aagcccgccc gcgacgctcg gccttctcac ggtgaacgat gaccgaaaga
    22501 gcgtccacgg gcgtgccatt gaccaggata tccatcttga ccagatcgtc ggcatggtag
    22561 ccgtgcagct cgtagtccat ggtcccgtag cccttggttg ccgctttcag gcggtcgtaa
    22621 aagtcgaaga tggtctcggc caatgggaac tcataggtca gcatcactcg cgtgggcgag
    22681 aggtactcgg tcttgaggta tttgccgcgg cggtcctcgg cgatcttcat gatcgaaccg
    22741 atcgaatcgg ccggcacgat caggttggcg gtgatcatcg gctcgcgaat ctcctcgacg
    22801 accgacatgt ccggcaactc gctcggcgag tcgatgtgaa ttacctcacc gctcttcttg
    22861 agaatctcgt aggtcaccgt cggggcggtc tgaacgacgt cgactccacc ttcccgctcc
    22921 aggcgctcct ggatgatatt catgtgcagc aggccgagga acccgcagcg gaagccgaaa
    22981 cccagagcgt tgctgtgcac cggctcgaac gagaacgagc tgtcgttaag ccacaacgtt
    23041 tccagcgcct ctcgaagctg cggggcttcg gtcatcggcc cggggtaaaa gtcgcagaac
    23101 accatcctct gtggcggctg gtaaccgggc agcgccgttg ccgcccggtt gctttccagg
    23161 gtgatggtat caccgatgtt cacgtcgtga atggtcttga tgcccgcaat caggtagccc
    23221 acctcgccgg cggcaatcga gtcgaccgcc tgcggcttgg gcatgaactt gccgatttct
    23281 gaaacggtat accctcgccc ggcagccatg aggacaatct tgtcgccgcg cttgatggaa
    23341 ccgtccatca cccgaacgtg gcagaccacg ccacggtgaa cgtcgttctt ggcgtcataa
    23401 accaaggcgc gcagcgggac atcaatactg ccggcaggcg gccggatcct ttcgataatt
    23461 gcccggaaca attcgtcgat tccaaaaccg gtcttggcgg aggtgaaaac ggcatgctcg
    23521 gccggcaatc ccaggacgtg ctcgatctcc agcgcgacgt cttcgggcat ggccccgggc
    23581 aggtcgattt tgttgaccac cgggatgata tccagcccgg ccgcaaccgc caagtaggcg
    23641 ttggccaccg tctgggcctg cacgccctgc ccggcatcga ccaggagcag cacgccctcg
    23701 caggccgcca gggcccggga gacctcgtaa tggaaatcca cgtggccggg ggtatcgatc
    23761 aagttcagca tgtactcctg tccctcatgc tcgtggaaaa ccgtcgcccg gttggccttg
    23821 atggtgatcc cccgctcccg ctcgatctcc atgtcgtcga ggaactgagc gcgcatctcc
    23881 cgtgctggca cggctttagt ggcgaccagg atccgatcgg ccagtgtact cttgccgtga
    23941 tccacatggg caatgatgct gaagttgcga atcaggttcc ggtcaaacat cgtcgctggg
    24001 aactcccttt tgcgtgtgga ccctatagtc tatcgactat cggacagtta tgaaagagct
    24061 tgatgctggc tgtttcgcga cctttccgtt gcttcgcacc ttcaccgtga tagacttacc
    24121 ccttcggatc tgtcggtacg gacctcgccg ccactgctga gtaattgcca tgcaaaccta
    24181 cgagaaactg ggtgctttct atcttggtcg cacttatgac ctgcaacagc gccgcccaac
    24241 cacggacctg gtgctgtacg acagccgcga tctggtcaca cacgcggttg tcgtgggtat
    24301 gaccgggagc ggtaagaccg gcctctgcgt cacgcttctg gaagaggcgg caattgacgg
    24361 gattcccgcg attgtcatcg accccaaggg cgatctgaca aatctgcttt tgacctttcc
    24421 ggaattacgt ccgcaggact ttcgcccctg gatcaacgag gaggatgccc gccgccaggg
    24481 gcttgatccg gattcctacg ccgccaagca ggcggagctg tggcagcgcg gtctggctga
    24541 ctgggaccag gacggcagcc gcattcagcg cttgcgcgac tcggcggagt tcgtcgtcta
    24601 cactccgggc agtgatgcgg gccttcccgt ggccgtgttg aagtcattcg ccgctccgcc
    24661 accggaggtt gtgggtgatt ccgaggccat gcgggagcgc gtggccggtg taaccgccgg
    24721 cctcctcggc ctgttgggca tcgaggccga cccggtgcag agccgcgagc acgttcttct
    24781 gagtaccatc ctcaacaacg cctggaacca ggggcagggg cttgatatgc ccgccctgat
    24841 cgcagccatt cagaatccgc cgttcagtcg gctgggcgta atggagctgg agagtttcta
    24901 tccggccgcg gatcggttcg ccctagcggt ccggttgaat aacctgatcg cctcgccgga
    24961 cttcagccga tggctggaag gtgagccgct gaatgtcggc gagatgctct atacggcggc
    25021 tgccaagccg cgcgtggcca tcttctcgat tgctcacctc aatgacgccg aacgcatgtt
    25081 cttcgtttcg ctgttgctca gcgcgactct gggttgggtt cgcagccaat ccgggaccgc
    25141 cagcctgcga gcattgctgt acatggacga gatcgccggt tacttcccgc cggtgtcgaa
    25201 ccccccgtcc aaggccccgc tgctgacgct gatgaaacaa gcgagggcat ttggccttgg
    25261 ggtggtgctg gccacccaga acccggtaga cctcgactat aagggcttgg cgaacgccgg
    25321 cacctggttc atcggtcggc tcctgacgca gcgagataag gaccgggtgc tggatgggct
    25381 ggagggagcc gcggccggcg cttcgagcga attcgaccga gccgccatcg ataagatgct
    25441 ctcacagttg gagaatcgcg tcttcctgat gaacaatgtg cacgaggata cgcccgcggt
    25501 tatccacaca cgctgggcaa tgtcctatct tcgcgggccg ctgacccgcc ggcaaatccg
    25561 gatgctgatg gatccctatc gccggattgt ctcaaaggag gctccggtaa gaatgcctca
    25621 gcgtgaaggc gctttgcccg cggaggctgt tccatctccg accgtgcggg tgccggccga
    25681 gaggacttcg aggcccatcc tgccccccgg cgtccgacag gcattcatcc ctgcccgctc
    25741 ggtaccggcc ggtccaaacg cggtatatcg ccccatgctg ctggcgaccg cgacgctccg
    25801 ttacgctgac accaagctca gcatcgacca ggaacagcga gtggtttact tggtgccgct
    25861 gatcccacgg agcggctcgc cggtggaatg ggactccgcg caaacgacgg acctcggcga
    25921 ggccgacctt gatcctgacc cagccgctac ggcctccttc gccccgttgc ccccggaagc
    25981 cgccaacgag cggtcctatc aagcctggaa aaaagacttc gcggatatgc tctaccggag
    26041 atctgttctg gagctgcttc gatgtccgag tctcgagata gtgagccgcc ccggcgagag
    26101 cgagcgcgag tttcaactgc ggatctcgca agccgctcgc gagcgacgcg acgacgaggt
    26161 cacgcggctg agggagaagt atgcttctcg gatgaacgtc ctgcaggaac gtctgcgccg
    26221 ggcccggcaa gccgtggagg ttcaagagga acaatcgcgg gcctcaaagc tctccacggc
    26281 cgtctcgttt ggcggggcaa tcctctcggc cctctttggc agaaagacgg ccggcgttct
    26341 gaacatcggt cgtgcgggca gcgccgtccg cggcgtagga cgctccatga aggaggcagg
    26401 agacgtccgc cgggcagagg aatcggtcga ggcggtgcag cagaagatcg ccgatctcga
    26461 ggctcaattg cgggcggata tcgatgcgat tagtcagcag accggcgagg cgttggagca
    26521 catcgcagtc cgtcccaaaa agtcggacat cagtgtgcgg tccgtcctgc tggtgtggga
    26581 accgtgctgg gatacaaggt cacaagtggg tggctgaacc attcgcccgc cgttctcggc
    26641 ttcctgcaaa cggtgaaagc aacgaaagct cacgcggccg ctggcacggg ctcacgacga
    26701 ccccgcggtt gtcgtgttcg cggtgccgat acgcgaggca cggagcgttc agggcttcgc
    26761 gacgctgctt ccccccaggc atgcagacta atgccaacga ccagcccgat gcctaccacg
    26821 gcaaagagga tgcccatcca gatggaacca tagggaacga tcgcggacca agtgccaacg
    26881 gaaagcgaaa ccacggccgc gaagcaggtc cacacaaaca ggatccaacg caccatggcc
    26941 gattctccgc taccattcga actcgtcgat caacgagaaa tcacggtgtc gcctgtacag
    27001 acgacgaaaa cgcagcgctg acccgatcat gctgatgccc actactgctg cggttgtggc
    27061 caccaggagt tcaagcaatc ccggaaacaa gatgacgcac aaaccgatgg cgatcagcag
    27121 aatccccagc atcagttggt gcttccacaa gaaacccagg tcagcagctt cgaaggcaaa
    27181 aaactcacgt actcgcatgg tttcacctcc tcgtcctgca gacgcgtact tgatctcagc
    27241 ctccgactga ccgcctactc tatctgtcct tctgctaccg ctcgcgattc cgcgctagca
    27301 aaagacatgc cagagaataa aacgggagca tcgttttaaa tggcttttct gtaaagactt
    27361 atgttcggcg ttaccgatgc aggatgccga cagtctgccg ttctggcagg atcggcgcca
    27421 ccgcgaccag gcaaggcctc tgcgctttgt cccgaaaagc agctctcttc gagccgagcg
    27481 gcaggcaagt tgttgatccg cgacaagcat gagcttgcct tcgcgcctca tgaaacgcga
    27541 ttcacggtcc gccgtggccg ggtcgatctt gacgtcgctg ttctctcgaa acgcactggt
    27601 cggcccgcag caacttcgat ctttgtgtca gatccgtggc cgttccgcca gactcttcgg
    27661 ctgtggctcg ccccgaaaac caagctgcgg ccggagccgg actgcctgca ccagtctgcc
    27721 gcacaaggtc gcaaagcgac aacttcgggc acgtgactga catgtaatcg tcctccctcg
    27781 ttttcatcga ctccacagcc ggccagcgcg ctctaaccta agtcggaccg cctctttgcg
    27841 ggtagcaacc aaaacgcact gtaatccgga aacatgtgcg caaaacgcgc aacagatacg
    27901 acagaagaga caaagcagat ccgcacgacc ggaaggatgc cccgaacgtc tgcccgacca
    27961 gttgatcggg gcgatccgca atagtcccat tttctttgtg gcaagctccc atgtagtcgt
    28021 cccagattat tcacgggctg gattcccatc gggaagcagg gaccgaatcg gtggctggaa
    28081 tctgcgtcga gatcggcagc acattcggtt gaagaggaga cttcgtcgtc cacttctgct
    28141 ttctcagcgg cttcgcaggg acctcagccc cccgcgacga ggaacccgga aaggcactct
    28201 gaacaacggt ccgagctatg ttttcggccc agtcgccttc gggtacaata accctgtcgc
    28261 acaaccggaa tgtctgattg aggccacaac atgcagcaca ccgcacaagc tccgccaagg
    28321 attcagtacc ttcgggtcca gaactaccgc gcccttcacg atctcgaact gcggaacctg
    28381 accccgctca cggtgttcgt cggacccaac gggagtggca agtcaacaat atttgacgtc
    28441 ttcgccttcc tttcggagtg cttctccgtt ggcctgcgca aggcgtggga caaacgtggt
    28501 cgcttcaagg agttgcggac ccgtggctgc gaaggtccga tcactatcga ggtcaagtac
    28561 aaggaggtca atctaccaat catcacctac catttggata tcgcggagga ttcgaaagga
    28621 ccgtacgtcg cgaaggaatg gctgagatgg accagaaaag tgggtaaggc gggtcgaccg
    28681 ttcaacttcc tggatttctc agaaggccaa gggactgtca tttccggcga gctaccggag
    28741 gagacagacg agcgcatcga tgagaaactt gattcgcggg agcttctggc cgtcaacacc
    28801 cttggccagt ttgccaggca ccctcgagtg ggtgccttgc gtcgctttat aacggactgg
    28861 tacctgtcct acctgaccgc ggacaacacc cgaggtcagc ccgaagccgg gccacaggaa
    28921 cggctctcgg cttggggaga caacctgccc aacgtcattc agtacctcaa agagcagcat
    28981 cccgagcgac tggaccacat tctgcaagtt ctatcgcgca gggtacctcg cctggaaaaa
    29041 gttgatgccg agatcatggc tgatggacgg ctgctcttgc agatcaagga tgcccctttc
    29101 gaacggccgg tcatggccaa gtttgcatcc gacgggacgc tcaagatgct tgcctacctt
    29161 gttgtcctgt atgatccgtc gcccccgcgg ctcgtgggca tcgaggaacc cgagaaccac
    29221 cttcaccctc gcctgctgcc cgaattggca gaggaatgcc gccaggcttc acaggccacc
    29281 caactgttga tcacgacgca ttcacccttc ttcgtgaacg ggctgcgacc gcaggagacc
    29341 tggatcatgt accgcgacga gaaaggttac acgaaagcca agcaggcgtc ggaaatggaa
    29401 ggaatcacgg aattcattga ggagggtgct caattgggat acctttggat gcaagggcat
    29461 ttccgtgttg gcgacccgct ggcctccaaa ccgtcctcgc cgccagccca agcgaagata
    29521 ccgcatcgaa agactgagcg accctgacgg aactgaccgc gatgcacatt gagttccttg
    29581 tcgaagaact ctccgccaag gcagctcttg atgagcttct gcctcggttg ctggggaccc
    29641 aggcaacctt tgacgtgcac gcgcaccagg gaaaacacga ccttctggcc tgcttgccca
    29701 gcaagataag ggcctacgcc gggtttcttc cggcggactg gagggtggtc gtgctcctcg
    29761 acctcgaccg ggaggactgc aagaagttga aacgcaagct cgatcgcatc gttggcgatg
    29821 cgggactgag aacgaagatg tcctccgggg ggcaagccgg atttcaggtg ctcaatcgga
    29881 ttgcgattga ggagctggag gcctggtttt tcggcgacgt tgccgcatta catgccgcct
    29941 atccgcgcgt ccctctgacg ttgggccaaa agagcggctt ccgagacccg gatcggatca
    30001 agggtggaac ctgggagcgg cttgaacaag tcctgcagag acacggctac ttcaagagcg
    30061 gctacccaaa ggtgacggcg gctcgggaga tcgccaaaca catggacccg agccgaaatc
    30121 gctcgcgaag cttccaggtc ttccgcgatg ggctgcgggc tctcctcgaa tcgtagggcc
    30181 ctttcagtcc ccgcagggtc gctcgaatgc acaatgcgcg gtcaaccgac cagactcgca
    30241 gttgaagtca actctgtcgc ctgactcgcg ttccagatgg agaatcctcg accagatagc
    30301 cgctggcggt gagcacgtcg cggattctgt cagcggtggc aaagtcgcct ttcttgcggg
    30361 cttggtcgcg ttgccggaca agatcctcga tttccgggga gatgtgcgcg gggggctcgg
    30421 cggataaaag aatgccgagg accgcatcca ccgcccacag ggcagccata acttctttgg
    30481 cgtcttcctg gaagagccga ccctgcgtca tcaagtaatt ggcctgccgc accaggcgga
    30541 agagtgcggc gagcgcgact gatacgttca cgtcatcgaa caacgcttcc cgcagagact
    30601 ctctcatcgc cagtatggaa ggtcccagct cgggcacgcg cggccccgac gtgttgaccg
    30661 cagccagctt ggcaacgcac tcgtcgagcc tgcgaagcga agcagcggcg gcatcgagcc
    30721 cttcatcggt cagctcgatc ggctgtcgat agtgcgtctg caggaggaag aagcggattt
    30781 cgcgggccga gtaccccttc tcaagcaggt cgggaagggt aatgcagttg tcctgcccat
    30841 aggccatctt cttgcccttg gccagcacca gttccgagtg caaccagaaa cgcgccggcg
    30901 aatcgccggt aagtgctctg ctttcggcca gttcgttctc gttgtgcgga aagaccagat
    30961 cggttgcggc ggtgtggatg tcgaagcggt cgccgagttt gccccgggcc atcgccgcgc
    31021 actgcacgtg ccagctcggc cggacgttac cccattcggt cctgtggctg agacccttcc
    31081 gcatctcggc cagggtgcat ctccgcatca gcgtgaaatc acgaggatca ttcttgtcgt
    31141 accgctcgag gtccacggtg gcgccgattc ggatcttgcc gagatccttg ccggatagct
    31201 ccccataact ctcgacccga ctgatgttaa agtaaaccga atggttcttc tcgtacgcaa
    31261 agcccttctc gaccagcgac cgggtcaggt cgatcatatc gtccaccgac tccgatgccc
    31321 gtgcgtaggc ggaggcaggt ttgatgccca gagcccgcag atcctcgtgg aactcagcct
    31381 catgctgggc acacaattcg gacatggctc gacccgtgct ttcggccgcc aggatggtgt
    31441 tgtcgtccaa atcggtgatg ccgacgacgt gctcgacgtc ataccccgcc agctccaacg
    31501 tcctgcggac gagatcggcc gccagcattc gcctcaacac gccaagatgc gggcgggcgt
    31561 gaaccgttgg accgcaggaa tacattgtca ctttggcacc gcttgatatc ggttcgaacg
    31621 cttcgtactt gcccgtgagg ctgttgaaga agtggacctt cacaggcgcg acctcgggct
    31681 cggccacctg aaacagcgtc gtcgacaggt atcgctcgcc gccgtcgggc aagatcgcca
    31741 ccaccacgcc ggaatcgatg ctctccgcaa tctggcatgc cacgaacatg gccgcgccgg
    31801 agctcatgcc gaccagaagg ccttcctccc gagccagtcg gcgggccatc tcgtaagccg
    31861 cgtcgtcctc gatgttgatc ttctcgtcaa tcgcggagcg atcatagatg ccgggaacgt
    31921 aagcctcctt gagattcttg agtccctgga tgcggtgccc tagatacggc tcgacgccga
    31981 tcacccgaac ggccgggttc agctccttga gccgcttgga gcacccgacc accgtgcctg
    32041 tggtgcccat gctggcgaca aagtgggtga tccgttcctg ggtctgcgcc cagatttcgg
    32101 gggcggtggt ctcgtagtgt gcgagaagat tggcccgatt gttgtactgg tcgggaagga
    32161 aaaaccgatc gggctcccgg gcggctaatt cgtaagcttt ttcgatggcc ccgtcggtgc
    32221 cctgatcggc aggcgtgagc aggaactccg ccccaagagc ggccaggatc ttccgccgct
    32281 ccacggagac gccctcgctc atcgccaaca ggatcgaata acccttgatg gccgctacca
    32341 tcgccagccc gatccccgta ttcccggatg tcggctccag aatggtcttg tcccgcgtaa
    32401 gctggccgct tcgctcggct tcttcaatca tgtaaagggc aatccggtct ttgaccgaac
    32461 cgcccggatt ggcccgttca agcttcgcgt atagttcaac cctcgggttg ggattaagcc
    32521 ggcggatcgg cacaagaggc gtgttgccga tcaacgaaag cagccccggc cgatccggca
    32581 gattgcgctt gccgggggac gtggattcca tcttgctgtc attcttcgcg gtcataacca
    32641 cgcaatcgta ccgcattcgg aatgcaagat cacctgccgt ctcggcggct ccttcccggc
    32701 gagccagtga aactccaggt ggtcagacgc tgcggtttct tcagaaccgc gagcgtgctt
    32761 gccacgccaa agcccggcga aggcgggagg gagcggccgt cctgtgacgg gtagcccgag
    32821 tctggtggac ctgggattgt ccaagcctct attggccgac cacgatctcc agcggctggc
    32881 cggtcgtagc ggggtgcgtc acatggatcg tgtcgacgaa ctgagcatcg gtcagggtca
    32941 ggtggtacgt tccaggctcg ggcgagatgg tcagtgacag cgtggccagg actgtctgac
    33001 cggcgggtag cggttctctt gtcacgccgc cgatgccggc cgtggttgtg aaccactggg
    33061 ctgactcctg cgactcgatc ggaggctcgg ccgttggggc tgcgatgggg ccgttgacgg
    33121 ccgtcaggtc ctgcgtcagc gcccagacgg tcggcggatc gaccccgagc agggagtcca
    33181 catccatgcc ccacgccgtg acgtccgagc cgtcaggcgt gagacgcacc catagccagt
    33241 ccatcaccgt caggtcgtag tggctgctct gggcatggcc gtctacgtca tgggagaaga
    33301 ggacgttgtc cagttcggtc cagtcggccg agtcgatcga gaccacattg gccgcatcga
    33361 cggccggctt gccctcgaag ccccagatcg gggcgtcggt gatgaccacc aggtcaacgg
    33421 agatggtccc gcccgtggcc ggtagcgtca ccgacgtcgc tccggttccg tgcaccaccc
    33481 actgggctga gacattggcg cttggcgtcg ccacctgctg ctgccgcaat agggtcaaca
    33541 ggtcttgctt ggcccggaat cctttccggg ctgcctctgt ccgcggaatc ctttccgcat
    33601 tcggcggcaa tgaggatggg aatcctcgac cagaaggccc cagaaaggat tctggggcca
    33661 gcgatgcgtc agaagtgccg acgagggcgt cggcaccacc ggcaaggcca acgcctccgc
    33721 ccatgcccat catcatcatt ccgccggggc aggcgacggt gttgttcagc aggctgcttg
    33781 cgatgctctc gtccgtggcg ttgatgacac cgtccacgtt cacgtccagg cggacgttgg
    33841 acgcgtcggc ggtgtatccg ttggccgcgg cgatggcgct gacgtcggct tgctcgacga
    33901 tcgcattgcc atcggcgtcg gccgcgagca tgcgaatcgc gcaatcggcg tcgcacagaa
    33961 tctcggtggt caggtctgca tccacgatcg cgttggacag atcgatggtg tagcacacct
    34021 gatcgttgag gcgattgtcc gggtcagtcg catcgaagaa gagtcggaag gtctgatcgt
    34081 tgttgatgaa atcataagac ataggccacg gctggctggc atcgctgccg gtgacgtaca
    34141 caaagccaag gtccgtccat ggataggccg gctgggccaa gtccacttcg atgatctgga
    34201 cgtcgccctg acgcggttcg atcaccgcct cgttcggcgc cgcgtccgga tccagagcaa
    34261 tccccaacga cccggccgcc ccatgcgtgc gaacgctccg ccaagccgtg atggtgaagt
//
LOCUS       DAOTPW010000022        34312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1355_length_34312_cov_4.880229, whole genome shotgun sequence.
ACCESSION   DAOTPW010000022 DAOTPW010000000
VERSION     DAOTPW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 34312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34312
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1355_length_34312_cov_4.880229"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="P5316_03300"
     CDS             <1..403
                     /locus_tag="P5316_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84013.1"
                     /translation="GQRIRLKTTIVDDEGETIDESQLAPITQMFLEALRTYGAYLVDN
                     AGSMAFSAEDIHSANLDMTDDEVNDLISQPTGTPLPTGKTRWQVVIEKLNEELELIPV
                     AWSANPNQSAATATFDVANIEVVENATVPPG"
     gene            466..1392
                     /locus_tag="P5316_03305"
     CDS             466..1392
                     /locus_tag="P5316_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRR84014.1"
                     /translation="MNCSQRRVAHLPISGLLLLGCLLLTAMLSAASCVPSTDDPDGDG
                     DPIDPNNPPITDGSWYRPAVTATWQWQLQPNTAGNINTAYDVEVYDIDLFDTPDSVIA
                     DLHAQGRKVIAYFSAGTYEPFRSDANEFLPAEIGNSLEDFADERWLDIRSENVRRIML
                     ARLDHAMLRGFDGVEPDNVDGYANNSGFPLTAADQLAFNRFIANEAHKRNLAVGLKND
                     LDQITELVQYFDFAVNEQCHEYNECDLLKPFIDAGKPVFSAEYAGRYVNLPLIRNQMC
                     ADSLNLGLRTLVLPVDLDDSFRFSCDPSGPDY"
     gene            complement(1386..1970)
                     /locus_tag="P5316_03310"
     CDS             complement(1386..1970)
                     /locus_tag="P5316_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84015.1"
                     /translation="MTFRLSTAVLLLVVATALAAGGCTPPDNRGDNGDYDPVPGGQGI
                     PEGTYAGTLECTETTSLVQEGQDETLLGQTTPSFSVAFSFDDSGGLLDSNRDPIVVGS
                     TDSTTISGQTATGTVRSVDKAVDRLEVVADATATVEVTGYGTLPMIGVVTAVYEFAEP
                     NTVTLVTDKVFTSNLVDSQFIKIVAECSATLPYQ"
     gene            complement(2155..2778)
                     /locus_tag="P5316_03315"
     CDS             complement(2155..2778)
                     /locus_tag="P5316_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRR84016.1"
                     /translation="MNESPKRKPLLREDKPRAPHLPDRGYWGIGVYHPKKEHNFGTIL
                     RNAYAFGASFVFTVGPRFRLQAADTARAWRHVPVFRYRDVDDLRDHLPYACSLVGVEI
                     VEEAVYLPRFSHPERACYLLGAEDYGLPREVVACCEHAVRIPGAAYCLNLATAAAIIM
                     YDRVSKRATRVFGPLTALNTPQLNPPIHKTEASGSGVEAANRPCPPG"
     gene            complement(2981..3427)
                     /locus_tag="P5316_03320"
     CDS             complement(2981..3427)
                     /locus_tag="P5316_03320"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRR84017.1"
                     /translation="MATSQQGYGMMTLALFLSRCSLGLYFFFAGIGKIKSGVEAFYKG
                     GFTSLKPLWLPDWFAMPYGHALPFVEVLVGVALIVGFYGRLAAAVAALALVSFTIALY
                     QAGAFFPEKGGPFHSNVILLTLAFLLAVTGPGTFSVDSFRGRSTSR"
     gene            complement(3689..3880)
                     /locus_tag="P5316_03325"
     CDS             complement(3689..3880)
                     /locus_tag="P5316_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84018.1"
                     /translation="MKIELTPEEREVLLALVEREIADLGPEIRHTRTRTYRDDLKDQQ
                     RTLRNLFQHLREPQQVEHV"
     gene            4594..5313
                     /locus_tag="P5316_03330"
     CDS             4594..5313
                     /locus_tag="P5316_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRR84019.1"
                     /translation="MPYRNMSLDDFARYVGMDARDVKRLADRGKLPGQKIGGEWRFNR
                     ARVTEWLQQEMHTLGEERLIALERGIRSSTGQSSATDELTVTDMIGLEGIDPSLPART
                     RASVLRELVNLADRTGLLYDPTGLLSALEQREEMCSTALPNGVALPHPRQPMPYVSAE
                     PLICVGRVPKGIGFGSTSRELTHLFFLICCHQDHHHLRVLARLVRILVPETISTLLAV
                     ETREEMLQILIAAEKEIVTER"
     gene            5404..5742
                     /locus_tag="P5316_03335"
     CDS             5404..5742
                     /locus_tag="P5316_03335"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HRR84020.1"
                     /translation="MCDASWGAEEFISEPIIPASGTGDIMAMARSEPGLPARFTWRQR
                     EYVITAVLKAWKTSSPEGGTGRLYLRKHWYTVQTDVGPRMTLYCERQTRNRRNPKARW
                     WLYSVQGHTR"
     gene            5835..6674
                     /locus_tag="P5316_03340"
     CDS             5835..6674
                     /locus_tag="P5316_03340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRR84021.1"
                     /translation="MARADGTSRPHTAAGWLASLLVFFIGAVYVCTWWGLGIAWARLW
                     LPPILARSWGGISRAVRRWEGDSFWRWLLTQYMIALVPVVICAVLGRKPRDLGLGRFN
                     VLGWRLIAVSVVLSIPFGLLIIWSDPGSIPLPQTWSEAASYSGRLLAIVPEHVLVCGL
                     FVALMLPDRRLPARVPVAAVNGAWRRRALRWLGLAQPVEGSRVLAWFGLTGTSLVAVV
                     TSGILFWLAHLGKPNIMEVSLSLPGGVAVAYVTLRTGSVWPAIIAHFTMNLIPGGIAM
                     LFG"
     gene            6825..7451
                     /locus_tag="P5316_03345"
     CDS             6825..7451
                     /locus_tag="P5316_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRR84022.1"
                     /translation="MARIGPAVTVFGSARTKRGARTYKLGVDVGRRLAEAGFAVITGG
                     GGGIMEAAHRGAAKVGGTTVGLNVVLPHEQKPNPYIEIEVDFDYFFVRKVMFVKYAVA
                     MVCLPGGFGTLDEFFESMTLLQTQKTPPSPVILMGRDYWRPLLKWVRTTVLNKYQAIN
                     PEDMNLFTLTDDPAEAVDIIVKKYRQTGTLWEHPVRSHRRRAPTKPKD"
     gene            complement(7700..7773)
                     /locus_tag="P5316_03350"
     tRNA            complement(7700..7773)
                     /locus_tag="P5316_03350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7737..7739),aa:Val,seq:cac)
     gene            8224..10626
                     /locus_tag="P5316_03355"
     CDS             8224..10626
                     /locus_tag="P5316_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84023.1"
                     /translation="MGRKGHKSGRNPIIEYRRLSTDDLRRRIEQCREKGDTRTAIDMA
                     KECHRRQPSPENSDLLGHLYLERGRRLIEKNLFAEALMVLNNALSLGQGSADLLWLMF
                     ECGLRSRQYETALSALRRLNDPAEQSRASRYLADEAIARGESLDRFCEPATREDVHRI
                     RRAFAAFEKGDDPAVTDELRGIGLTSPCAGWKWLLLGLAAYTRGDRDRARLSWDKCGT
                     EGRPGRLAAILRSGLLNDGRDGELTPALRERLIEHFGSPRLALLTQIKERLAGKDVRG
                     ALDGCARLLARVEASEREAFAQRLGRAICEKMGNDEDTVQRFRRVLGPLPEDPFLYRS
                     AALSLEGSFPSDAIDVWSCFIDTLEHVKGIAPHLFPRAKGLVLQRMGDLAMQASKVDG
                     FEAFLGLASGRCNEAIGYYRQSLQEYAEDKTSHEKLMAAYLLQNQPKKAERHAREVLR
                     RWPNDVNTLLFLGLRCIDRAAFRKALDYLKQALRVDPADSQVRAGIVLCYLKSARRRL
                     RQGNLDQARQDYEEAQAVLDPTASKALFFCRWAALEWRAEKPDRAQELLSQALAGREE
                     RVVVYFVLAIELSVAQAPPEVQQRFDKLLEEALADEPSGAAAARMAEYVLQMAEEGVD
                     YPKSRIHADLLVGYVSRAAGRSTFSELQLLQVCEYLEDRQEWKLLERLAHQGCDRFVG
                     NPHFPVFLGRSQRGLGRRRLPARTRRLLERAYEQAQAQHRFDLSAEISSLLDEPQPSM
                     SHGFGDFRSLFNALAHMLDASEAEDDTGRKPSTSRKKPRSKGIDMQRIFEWMQEGMDE
                     EN"
     gene            10719..11015
                     /locus_tag="P5316_03360"
     CDS             10719..11015
                     /locus_tag="P5316_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRR84024.1"
                     /translation="MANPYSILNVRPEAGDEEIRRCYLEAVHRFPPEHCPEQFVSIRQ
                     AYETIKDEGARLEFLLFDASQGESIDELIEEERCRTTPKRAGLAALLSLLGETR"
     gene            11048..11743
                     /gene="grpE"
                     /locus_tag="P5316_03365"
     CDS             11048..11743
                     /gene="grpE"
                     /locus_tag="P5316_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRR84025.1"
                     /translation="MLSTLRQWLLESEQWRHALLAPQENVDAAARVDLHTLLSELIAL
                     KQEVRLEARGTKTARQDLDRAIDEFHEGIEEVSAQTQKLFDPLLRERDRLRDGLNSQM
                     DSQLRSWVEVLLDLREALSRGEETSRQAARRVGWRRWLLPRGLLEGLHEGYMLGLRRI
                     DAILEARGVRPIECLGKPVDPEQMRVVDLVRRDDLPAGHVAEVVRAGYTCDSKVIRYA
                     EVRAVARQEGETA"
     gene            11740..13506
                     /locus_tag="P5316_03370"
     CDS             11740..13506
                     /locus_tag="P5316_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRR84026.1"
                     /translation="MSSEPIIGIDLGTTNSEVAVLENGRPRVLTENGQAILPSMVGLA
                     DDGRLLVGEPARNQWLLAPDRTIRSVKRRMGEDVKLRMGDQEFSPQEVSAIILRTLKN
                     RAERHLGRPVGKAVITVPAYFNDAQRQATREAGELAGLEVVRILNEPTAASLVYEAGQ
                     SAPQKVMVYDLGGGTFDVSIVRIEQGVIEVLSSHGDTHLGGDDFDALLIEHVAGRFQE
                     QHGVDLRADPRAHSRLWRAVEQAKILLSTTPYAQVNEEFICEKDGIPLNLSMELSRGD
                     YEEMIRPLIDKTMEAVQVALKDAGLTAGQIDRIVLAGGATRTPLVSEELRNRTGRHPH
                     QEVDPDLCVAMGAAIQAGIIQGESVSAVLVDVTPHTFGINCLGERFGLPYPYCFSPII
                     HKNTPLPASRTEVYYTVCEDQERAVIEVFQGEHPDALKNVRIGRFTIEGLSKGPPQSP
                     ITCRLDLDLNGMLKVTAQEKKTGRQKQIVIDNAMSRFEKTEMADAARRLRVLFGDDEQ
                     EQASAPEADAVSEAAESDHPAREVVRRAHAALKDVAGKDREEVLGLLQRIHDAIEKND
                     REDADRAAQELDSILYYLEESK"
     gene            13503..13868
                     /locus_tag="P5316_03375"
     CDS             13503..13868
                     /locus_tag="P5316_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84027.1"
                     /translation="MNDLSGSDAFGKGRERCPTCNAPRQAESVCYRCKSDLSSLIVLE
                     SRVDNLRAHARRCYARGWYRRAAELTRELLSLEACSEDLALLACASLMSGDFPSAVHA
                     YRRNVFLSQGNSSCSMSDT"
     gene            complement(13808..14806)
                     /locus_tag="P5316_03380"
     CDS             complement(13808..14806)
                     /locus_tag="P5316_03380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR84028.1"
                     /translation="MIGRPAPVLRLPPAVPIERVHGCSLMPMLPGARAVRLLRRKSFD
                     LLLMWDWSPYLDFSREGSDQPSLTILSSPIGMPGILFGHLMRRKRAQQTNFVCLSDSV
                     REQLIRLGLKSDSVRVIRPSVDPAEIRHADRQRVRSEMGLPADARVLLTACPPGRKGG
                     QYAAVWAAAILHQIWPDVRMILPGGFRGDGRARHLSEECYCPQIFRIAAEQYGPADLL
                     AASDMFIWPDDQSHSTGWLAWAMAAGIPIVATDGPSVRELIRDGQTGFLVGSSQPHAL
                     ATRIRTAWEDAEARRRCAQEALRQADQMCCAQKCLDAYVSLMEQLLLPCERKTFRR"
     gene            15064..16350
                     /locus_tag="P5316_03385"
     CDS             15064..16350
                     /locus_tag="P5316_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRR84029.1"
                     /translation="MGFKILLLLILLLASAFFSGAETALFSLSRHELMRFRQDKRPSR
                     QMVADLMRHPRRLLLTLMIGNVSINLFIFAASLSLSQSVLGRNSALVPIIGLASPILV
                     TLFGEILPKGAAILLRVPLAPRLAPMVRFTQVILTPFRALLGTLLVEPLTRLLVGTPK
                     PDEYVTVEELRELVEMSERHRVIDAHENAMLDQVIQLGELRTKDIMIPRVDMIAYDVH
                     GNPHDLERLMRKHEFTKIPVYDKDIDRIAGIIYAKDLLLHPGRPLAQLIRPAHYVPEV
                     VTITQLIEEFRRKKTQLAIAVDEFGGVVGLVTVDDIAKLIVGGLGTEGESEQDRLWVR
                     LDERRYRVSGGMSIHDWSEQFKMSRLDEEVTTLAGLILARLGRLPAVGDKLRLGNLVL
                     TVEALRGRRIEWVLLELVDGGSSAAPVDGKPGGDCP"
     gene            16347..17336
                     /locus_tag="P5316_03390"
     CDS             16347..17336
                     /locus_tag="P5316_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRR84030.1"
                     /translation="MIALLLGTFATAVTLAISGFFSGVEMGLYCVNRVRLRLRAERLE
                     ALDARALIDLTDRQDESILGIVLGNNLANYLLTVAATFLMACGIGVESSKVEFYTAAM
                     LSPLGFVFADVVPKNWFRAEADRLMYRCARPLRAMILVLRQTGILWLLGQVTRFSIWL
                     SGHEAESRMHGPRREVLGLLREGAAEGALTTEQAEIVERVMNLSSVRVGSIMIPRRRV
                     ASVPITANRSQFLRAVRSHSYSRMPVVDRDGKTVSGIVSVCDVLADDTDASISNFMHP
                     PIALQAQDSAARAIIQLQRANETMAVVNDPRRGFVGIITLKDVIEEIFGELPA"
     gene            complement(17478..18248)
                     /locus_tag="P5316_03395"
     CDS             complement(17478..18248)
                     /locus_tag="P5316_03395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR84031.1"
                     /translation="MGLLDGEVAIITGAGTGIGAATALRFVQEGASVVMVGRRREKLE
                     ETARAAGRTDRLAVVPGDVRDQKTVDAAIAAATKLGGLHVVVNNAAVAYPVGFPDADF
                     ETWRDIFDIIVVGAFRFCREAARVMIANKTPGRLVNVTSIHGTQAEPGASGYGAAKAA
                     VNQFSRALSVELAPHNIRVNAVAPGFVDTPMSVYNGRNELESDWFKRFYVEARKIPMA
                     RAGRPEEIASAILFLACRESSYITGHVMIVDGGLTVTF"
     gene            complement(18260..19255)
                     /locus_tag="P5316_03400"
     CDS             complement(18260..19255)
                     /locus_tag="P5316_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HRR84032.1"
                     /translation="MGYVRFEKDWKLNECSVLRIESDVLRIDVLPQLGGKIYHWVHKP
                     TDRDFLWQHPRIMPRIMPSGMNYDDNFSGGWDELFPNGEAGPYNHEMYPDHGEYWTRP
                     FEWEVLQSRTELTLHLWAEGGVTPTRMERWMTLAASSPVVRVKYRLSHLGRHGFDYVW
                     SIHPALSVGPQSEFVIPAGKGIIGSPGLGRLAAEPLEFNWPQVPGRDGKLFDMSRVPA
                     GVDVPHFEMVYLTELHEGWYAHLDHATRSGFGLAFDNQLFNTLWLFATYGGWRGLCTV
                     IPEAATGFPGDLATASTSGRCGHLDSGQVVETETAAVVFTGRDRIEHISIDGRVR"
     gene            19346..20998
                     /locus_tag="P5316_03405"
     CDS             19346..20998
                     /locus_tag="P5316_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRR84033.1"
                     /translation="MLASSYQPVDYAIIIIYFILTLAIGLWSARKAKGGSKEFFIAER
                     SLPWWVVGFTMVAASISAEQMLGEVGYGFGAGLVVSNWDLGVYPSLTLMIFVFLPLYL
                     RSGTTTIPEYLEKRYNKTTRLLFAVYTVFNNACVTLVMVLALGATAMKYFLGIPPILG
                     TAILAVFTGIYTVYGGMSSVAWTDTFQCILLLAGGLTLFGAGLNEVPGGWSGLLERMR
                     DAGSAHLIRPMDDPYVPWPGVILLMLSTNVWYCCTNQFYVQSCLGAKDERHGRMGVLL
                     TAFLGPVLTLCFAFPGYIASDLLKQGVLPALPLTPDGQPDANATYPHLAQFLLGPGLR
                     GFLVAAVLGAIMSSVSAIVNATASVYTIDIHRRWLRPAASDRELVRVGRLVGLVTLLV
                     AFPLTLLASSYRYIFTYSQNAWCILAIPIMLAFTYGALWKRTTAKAATATFVLAAPFV
                     AVPFVWGNPDDNWLRLPFISGRIHLFNFAFVLWIAAAVFMIAVSLLTRPAPAEAVAPY
                     VWRPSLNLPSPEHRGQYPWYQTVGFWAVIAAIMYLIIYVIFW"
     gene            21008..21727
                     /locus_tag="P5316_03410"
     CDS             21008..21727
                     /locus_tag="P5316_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84034.1"
                     /translation="MSRLIALFVLFMPLFGCVRMSGSASHRASPPVFPGLPLLVDEDF
                     DKPSTQWELDDPRAWKFTRDGDRTVLCLAGNSSYKPPYRSPTGMALLKDVIVSDFVLD
                     ARVRSTKADYPHRDMCLFFGYQDAARFYYVHFGLKSDDASNTVHIVNNADRRPIVKTR
                     TDGTPWTEGYHHVRVVRKVAAGTIEVFFDDMTQPCMTAEDKTFTWGRIGVGSFDDAGN
                     VDRVILWGRKVSLPATRTVVP"
     gene            complement(21772..22170)
                     /gene="atpC"
                     /locus_tag="P5316_03415"
     CDS             complement(21772..22170)
                     /gene="atpC"
                     /locus_tag="P5316_03415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRR84035.1"
                     /translation="MAEKTFKCSVLTPEARVFEGDVESVVLPAHDGEIGVLFCRAPLL
                     CKLGAGSLRLRKGTETQTWFVDGGFAQVIENQVTVLTQKALRPEEISAADAQARLEEA
                     LKMPAGDDAALRRKNRIVAGARAQLRMALM"
     gene            complement(22375..23841)
                     /gene="atpD"
                     /locus_tag="P5316_03420"
     CDS             complement(22375..23841)
                     /gene="atpD"
                     /locus_tag="P5316_03420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRR84036.1"
                     /translation="MAKSNGRNFGRVTQVIGSTLDAEFDEDRMPKLYNALKVNVERKV
                     LGMTERETLWCEVALHLGGGKVRAVALGTTDGLVRGAQIEDTGGPVTVPVGMQTLGRV
                     FNLVGEPIDMRGEVKAESRRPIHHAPPDFADLTPKSEMMETGIKVIDLLAPFVRGGKI
                     GLFGGAGLGKTVVVQEMIARIAREHSGYSVFAGVGERTREGNDLWLEMQEATFKDAQG
                     NVKQVMDHTAMVFGQMNEPPGARLRAALSALTMAEWFRDESGADTMLFVDNIFRFSQA
                     GSEVSALLGRMPSAVGYQPTLGTEMGDLQERITSTKKGAITSVQAIYVPADDLTDPAP
                     ATTFTHLDAFIVLARGISEKGIYPAIDPLASSSRILDAQYIGERHYKVAREVQQILQR
                     YRDLQDIIAILGVEELSEEDKLLVSRARKIERFFSQPFFVAEVFTGYPGNYTRKEDTI
                     RSFEEICAGKWDHLPEQAFMYVGTVEDAAAKAEKLAKN"
     gene            complement(23941..24666)
                     /locus_tag="P5316_03425"
     CDS             complement(23941..24666)
                     /locus_tag="P5316_03425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRR84037.1"
                     /translation="MILSLAPPPATQPATRPAEMKIGPGQQIVAMGDSITQAGGYLRA
                     MDAVFAQQYAGMKIPPIVNVGVGGQKAEDMVARFEKDVVRRSPAIVTVSVGINDVWHR
                     MADRPDPRVLEAYERNLERMVKMAQESGARVLVLAPTVIEEDPASEGNKRLAAYVAAG
                     KRVAQRNKCQYVDLHALFLKAIEKRKKDSGSQERFFTTDGVHMKPAGDVLMAVGLLRA
                     LGVPDEKMRATDLAKVFPEAGQP"
     gene            complement(24690..25568)
                     /gene="atpG"
                     /locus_tag="P5316_03430"
     CDS             complement(24690..25568)
                     /gene="atpG"
                     /locus_tag="P5316_03430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRR84038.1"
                     /translation="MAKARAIIKRRKAVINIRKITRTMQLIATARFQAAFNRATKTRP
                     YTERITDLVRRLSRGQGDLSHPLLKVNDQAKRSRLIVITSNRGLCGGYNGGILRSAIE
                     HLKVREQAGETVDIHVTGKKGISYFKFLGRAMAATDTRFEDKPQYVEIEPIAADVMAA
                     YERQEIDAVHVAYMRFFSAGVQRPHVMQLLPIEPPEPDTETGEAAAPVQYDFTPPPEE
                     LLAELLPETVRVRLFQCFIDAAVSEQVARMVAMKSATDAAGDMIRTLTQHYNRARQSQ
                     ITLELLDIVSGAEALR"
     gene            complement(25611..27272)
                     /gene="atpA"
                     /locus_tag="P5316_03435"
     CDS             complement(25611..27272)
                     /gene="atpA"
                     /locus_tag="P5316_03435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRR84039.1"
                     /translation="MKFKVDEIASVLREEIAKYKSEIDVAEVGKVLQVGDGIAQIYGL
                     SNAMAGEMLEFQNGAIGQVFNLEENSIGAVILGDYLGIMEGDTVRGTGQLLSVPVGEA
                     MIGRVVDPLGRPLDNAGVIETPLRRPLESNAPGIAGREPVNEPLQTGIKAIDSMIPIG
                     RGQRELIIGDRKTGKTAIGVDTIINQKGTGVICVYVAIGQKESTIAGVVDALRQHGAM
                     DYTIVVSAGSSDSAPLQYIAPYAGCAMAEYFMYEQGKATLCVYDDLTKHAAAYRQLSL
                     LLRRPPGREAYPGDVFYLHGRLLERSCKLATRYVLVPKSAPANYKPAINEKVYVGKPG
                     RAEAERDLKALGRTDYEIRKIPGSGGSLTALPVIETLEGEVSAYIPTNVISITDGQIY
                     LEPDLFFGGVRPAINVGISVSRVGGKAQIKAMKKIAGSLRLDLAAFRELEAFAQLGTD
                     LDAATQRQLDRGRAMVELLKQGQYAPMHVADQVISIFAGTQGFCDDVPLSRISAFEAA
                     LLKHVGDEYPEVREQLIQSGDLTDELRDRLVGIISDFKKRFMASK"
     gene            complement(27293..27892)
                     /gene="atpH"
                     /locus_tag="P5316_03440"
     CDS             complement(27293..27892)
                     /gene="atpH"
                     /locus_tag="P5316_03440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRR84040.1"
                     /translation="MAHTFDESMALADVYAEALLTAAAERGEEEAIAREMAEFMRFIE
                     REIDLCRMLAADSVDDEPRRESIERIFRGRMSDLLVNLLQVMNNRGRLNLLRKVGRCV
                     ELRMEEKHHQQEVIVETAMPLADDERQAIREEMSARIGKEAILIEQTRPELIGGVVIH
                     YGDIQVDASVASRISRTLRRMRERATEEIHGGRGYVLEA"
     gene            complement(27918..28514)
                     /gene="atpF"
                     /locus_tag="P5316_03445"
     CDS             complement(27918..28514)
                     /gene="atpF"
                     /locus_tag="P5316_03445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRR84041.1"
                     /translation="MTARAHNYMVIAVLHLATAGTLLASEDGRPNLFGGDLGNLVWSL
                     VIFMLVLVILGRFAWKPLLTGLQHREKFIHDSLAGAKRDREEAETRLKELTARLENAK
                     QEASAIVEEGRRDAEVVKRRIEDEARRNAEALIERAKREIGIARDTALRDLHEESAKL
                     AMEMAGAVLKRQLTPEDHRRLVGEALAKLKERGAESRN"
     gene            complement(28629..28940)
                     /gene="atpE"
                     /locus_tag="P5316_03450"
     CDS             complement(28629..28940)
                     /gene="atpE"
                     /locus_tag="P5316_03450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRR84042.1"
                     /translation="MVLAAPAMAAEAAAAGSDKAAEGKGFLLLQDKGASFLGAAIGAG
                     LVIIGGAAGIARIGGSAVESMARQPEVASQINLTTLITAAMVEGATLFAVVVCLLVLF
                     F"
     gene            complement(29123..30013)
                     /gene="atpB"
                     /locus_tag="P5316_03455"
     CDS             complement(29123..30013)
                     /gene="atpB"
                     /locus_tag="P5316_03455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRR84043.1"
                     /translation="MMMDLLATGSNPLTHVVDYAWKRVGHDGPLHTFTLISNHIIMMI
                     VAAVLLIILLPRLVRVPQRGDEITRMTPRGGRNVVETICEMLREFVAKPNLGPYTDRF
                     IPYLWTAFFFILTCNLLGLLPLEPITKPIFGHGIYGTATGNIYTTGTLAACTLVMVIA
                     NGLRIHGMAYVKHFFMGPFPINCLIAVLEVFGLLVKCVALAIRLFANMLAGHILLGVL
                     LSFIAMAGAASAALALLVVAPFVVLGSVAITFLEIFVAFLQAFIFTFLTAVFIGLAVN
                     IHSEHGEEQEGEEVRAEPGH"
     gene            complement(30010..30429)
                     /locus_tag="P5316_03460"
     CDS             complement(30010..30429)
                     /locus_tag="P5316_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84044.1"
                     /translation="MVSSSVVSTSKVPWLLCLPGMATVVLAAAGCYPTWRIGGRAGME
                     GMVAGQLLVVLVVYATLLPALRRMVSDDAAGRLQAGLKVGLIRLVLTVGAMVVIAWRR
                     WAEPKVFLTWVAIAYVVMVKLETVVLLRWNRVTGQRA"
     gene            complement(30423..30710)
                     /locus_tag="P5316_03465"
     CDS             complement(30423..30710)
                     /locus_tag="P5316_03465"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRR84045.1"
                     /translation="MSDPKSPWRLPPGIMQLAGAGVELAAGVGGACLLGWWIDRHFGT
                     RPWGLLVCAIVGIVGGLYNLIRRATHEMAEPPRARERKNRKDVGPDGTTSW"
     gene            complement(31243..31974)
                     /locus_tag="P5316_03470"
     CDS             complement(31243..31974)
                     /locus_tag="P5316_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRR84046.1"
                     /translation="MMSAERHFQRLMIPLLAGRRQECRDVLEEAMNEGLEARQVYRSV
                     IWPAMEHVAQMYREDRISLAAEHMAVRISRSLADQLQARLPRSEANGKRMLLTCAEGE
                     PEELGAQMCADLFEADGWDVYFVGGGVPDDEILELVGRLRPDILMIFGTQPGGVPGVR
                     RLIDLIREVGSNPTMNIMISGGVFNRAEGLWKEVNADLFAPNAAEALKIAASAQPRKP
                     EIRIPGAPKKRRRRRRPPLLAQLEA"
     gene            complement(32502..33752)
                     /gene="truD"
                     /locus_tag="P5316_03475"
     CDS             complement(32502..33752)
                     /gene="truD"
                     /locus_tag="P5316_03475"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HRR84047.1"
                     /translation="MLNVDELPYLTADVPGIGGRIKTRPEDFVVEEVPLYPPSGQGTH
                     VYFRVEKTGLSTMRAVAEIARALGRPPRDVGYAGLKDADAVTTQMFSVEHADPDAIRT
                     LSIPRMRIVDVSRHTNKLKLGHLAGNRFLIRIRDVDPARVGDVRATMEVLSKRGVPNY
                     FGPQRFGMRGDTWQIGRALLREDYDEMVQVLLGRVKPSDYGDVRRARELFDQGDLQGA
                     ANAWPYPFNNERRVCRMLIKFNGNARKAIRSIDMRLQRFYISAYQSQLFNQIVAGRIG
                     GLDRLMVGDLAWRHPQGAVFRVEDVAGEQPRCDAFEISPTGPLFGYRMTYPHGEPGQV
                     ETALINAEGTKPDEWREAGRHRVKGGRRPLRFQPQDVEVDAGQDDAGPYILLSMRLEA
                     GCYATTVLREICKTVGQGTEVGEE"
     gene            complement(33818..>34312)
                     /locus_tag="P5316_03480"
     CDS             complement(33818..>34312)
                     /locus_tag="P5316_03480"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRR84048.1"
                     /translation="DDLERVEAMAGLFAEAGIETMVRPSLKQARWEKLMWNIPFSTLT
                     VTAGGVTKDEIVNDPGLRQLAWTLMQETGRAGNAHGCSIDIPVMTEKMMSNTLDMPPY
                     RPSMLIDYQNRQPLEVEAILGEPVRQAAAAGVPVPAIETQYHLVSFLDRLNRGQINVS
                     ESRW"
BASE COUNT         6591 a        10668 c        10349 g         6704 t
ORIGIN      
        1 cggccagcgc atcaggctga agacgaccat cgtcgacgac gagggcgaaa ccatcgacga
       61 gtcgcaactt gcccctatca ctcagatgtt tctggaggca cttcgcacct atggcgctta
      121 cctcgtggac aatgccggtt cgatggcctt ctccgcggaa gatatccact cggccaacct
      181 ggatatgact gacgacgagg tcaatgactt gatcagccaa ccgacgggca cgccacttcc
      241 cacaggcaaa actcgttggc aggttgtcat cgagaagctg aatgaggagc tcgaacttat
      301 cccagtggcc tggtcggcca acccaaacca gagcgccgcg acggcgacgt ttgacgtcgc
      361 gaatatcgag gtcgttgaga acgcgacagt gccaccgggt tgaggagcgc caggcgagag
      421 ggcgcatggg ctgattcagg gacttaacgt gacggagaat ctgcaatgaa ctgttctcaa
      481 cggcgggtcg cgcatcttcc gatttcgggg ttactcctgc tcggatgtct gcttctgacc
      541 gccatgcttt ccgcggcgag ctgtgtcccg tcgacggatg atcccgacgg cgatggggat
      601 ccgatcgacc cgaataatcc ccccatcacc gacggctcgt ggtaccgtcc ggccgtcacg
      661 gccacctggc agtggcagct ccagcccaac accgcgggca acatcaacac ggcctacgac
      721 gtcgaggtct atgacattga tctcttcgac acacccgatt ccgtgatcgc cgacctgcac
      781 gctcaaggac gcaaggtgat cgcctatttc tcggccggga cgtacgagcc ttttcgttcc
      841 gacgcgaacg aattcctgcc ggccgaaatc ggcaacagcc ttgaagactt tgccgatgaa
      901 cgctggctcg acatcagatc ggaaaacgtc cggcggatta tgctcgcccg gctggatcac
      961 gccatgcttc gcggcttcga cggggtcgaa ccggataatg tggacgggta tgccaacaat
     1021 tccggcttcc cgttgactgc agcggaccag ctcgccttca atcgattcat cgccaacgag
     1081 gcgcacaagc ggaacctggc cgtgggcctc aaaaacgacc tggaccagat cacggaactc
     1141 gttcagtact tcgacttcgc agtcaacgag caatgccacg aatacaacga atgcgatctg
     1201 ctgaagccgt ttatcgacgc gggcaaaccc gtcttcagtg ccgagtatgc cggccgatac
     1261 gtcaacctgc ctctgattcg caatcagatg tgcgccgact cgctgaacct gggtctgcgg
     1321 acgctggtcc tgccggtcga cctggatgat tccttccgct tcagttgcga tccgtccggt
     1381 ccagattact gatagggcag agttgcggag cactcggcaa caattttgat gaactggctg
     1441 tcgacgaggt tgctggtgaa gaccttgtcg gtcaccagag tcacggtgtt cggttcggcg
     1501 aactcgtaca cggcagtcac gacaccgatc atgggaagtg tgccgtaacc ggtcacttcg
     1561 acggttgcgg tggcgtcggc gacgacctca agccgatcga cggccttatc aacagagcga
     1621 accgtgccgg ttgcggtctg acccgagatg gttgtcgagt cagtcgaccc cactacgatg
     1681 ggatcgcggt tcgagtcgag gaggcccccg ctatcatcga agctgaaggc aaccgaaaag
     1741 gacggggttg tctgaccgag aagcgtttca tcctgccctt cctggacgag gctggtcgtc
     1801 tcggtgcact ccaacgtgcc ggcgtaggta ccctcgggaa tgccttgtcc gccgggcacc
     1861 ggatcgtagt ctccgttatc gcctcggtta tcaggcggcg tgcaaccgcc cgcggccaga
     1921 gccgtcgcca caaccaacag aagtacggca gtgctcaatc gaaacgtcat gtctggttcc
     1981 ccatgtatca gcgagattca aagagccaca ccggtaatga tacgggcttg gctctgtcgc
     2041 ggcaagggcc ggcagagtcc gtgtccgtca cccgacaatg ccacagaaga gaatcgcctc
     2101 ggataacgcc ccgtggacga ttccctgctc tcttggaggc cgttcgacca ctcatcagcc
     2161 gggggggcag ggccggttgg cggcctcaac gccgcttcct gaggcttcgg ttttgtggat
     2221 cggcgggttg agttgcgggg tattcaaagc ggtcaatggt ccgaaaaccc gcgtggctct
     2281 cttggaaacc cggtcgtaca tgataatggc cgcggccgtc gccaggttga ggcaataggc
     2341 ggcgcccgga attctgacgg catgctcgca acaggcaacc acttcgcgag gcaggccgta
     2401 gtcctcggcg ccgagcagat agcacgcccg ctcgggatgg ctgaaccgcg gcaggtagac
     2461 ggcttcctcg acgatctcca cccctacgag gctgcatgca tacggcagat gatcgcgcaa
     2521 atcgtccacg tcccggtatc ggaagaccgg gacgtgccgc cacgcgcgag ccgtgtcggc
     2581 tgcctgcaag cgaaaacgcg gccccacggt gaagacaaac gacgccccga aggcataggc
     2641 gttgcgcaga atggtgccga aattgtgctc cttcttgggg tgatagaccc cgatgcccca
     2701 atatccccga tcgggcaggt gaggagcacg aggcttgtct tcacgaagca gcggctttcg
     2761 tttgggtgat tcgttcattg atcctgaatc ccgctcacct tgttcagtat accggctcgg
     2821 cgggcacgag agaaaccccg tcgagaatgc agacaaccga tctttgtcgt tggactgcat
     2881 cctgaagaca gtgcaggaca gagtctcagt gtgccattga ccgatgagct gcgggttcca
     2941 caccgggctt tcacgacgca caccctggaa tccggcagga tcagcggctc gtgctcctgc
     3001 cgcgaaatga gtcgacgctg aacgtccccg ggcccgtgac cgccagcagg aaggcgagcg
     3061 tcaggagaat cacgttggag tgaaatgggc cacccttctc gggaaagaac gcaccggcct
     3121 gataaagagc aatggtgaag ctgaccaagg ccaaggccgc cacggcggct gccaacctgc
     3181 catagaagcc cacaataagc gcgacgccaa cgagcacttc gacgaaaggc aacgcgtgac
     3241 catagggcat ggcaaaccaa tccggcaacc acaacggttt cagtgacgtg aagccacctt
     3301 tgtagaaggc ttccacaccc gacttgatct ttcctatacc tgcaaagaag aagtagaggc
     3361 cgagagaaca gcgactgagg aacaacgcca gagtcatcat accgtagcct tgctgtgagg
     3421 tggccatcgc atgctcctgt caatcaggac aaccacgtga atccgtgcca tggccttcgg
     3481 gcctcaaccc gaggtcagtt gggtgatagc ctacgacagg aaaggatgcc gtgccagtgg
     3541 caggatatgc tttgaccgag cccgggtttg acattttcat tcgtgcaacg gccgatcgtc
     3601 cgaggatggc gaacgcggcg gagacgacaa atcctcaccc cgtcgcgccg cccggtcagt
     3661 caagaaccca gacgacaaga tcggcgtatc agacgtgctc aacctgctgc ggttcccgga
     3721 gatgctggaa gaggtttctc agggtccgct gctggtcctt caaatcgtcg cgataggtcc
     3781 gggtcctggt gtgtcggatt tccggtccga gatcggcgat ctctcgctcc accaaggcca
     3841 agagcacctc gcgttcctca ggagtaagct cgatcttcat cgcacacctc ctggtcccct
     3901 ttaattctac cttcggatca gcgggacaat ccactttttt ctgctcggtc cgggcgaagc
     3961 ggagaagtct ttgacgtttg gtcagtgccg cgggggcagc cggcccgcag aggcaggcca
     4021 ggggagcttt acagtctggg cggctctttc aggacgcttg cgcgtcagcg aggaaccctt
     4081 gaccgatctc cataagtggt caagggcagg ccggcccgca aaagggccgg caccacacag
     4141 ggccgttgcc ccacggttct gaaaaagccc ttattggtac cccgccccag atgtcacgga
     4201 cacgcagccc agcgggtctt ggctgatatg gccgtgcttg gaagcgagac acgatctcgg
     4261 tttataggac catggaggtg ctaattgcga tgagctgtgg caaatacata acggatagga
     4321 cgttggccat gccgtgtgtc ctcacggctg cacgagcgcg tagcgtttaa ggacgacctt
     4381 cttctgaagg atgttgcctc agtagttctg gtggttgctc aggctgtcct tctaccgcgt
     4441 ccggaccgat tacaatattc caggccggcg ggcatccgca cggcgcgaga gttgtgctgt
     4501 ttccgtcaag gcttgtatgg ttggccgcgg tgcttgccgc gagccgggac gtactgccgc
     4561 aaagccttgg cgagacaaga ccgggttgta cgcatgccct atcgcaacat gagtctcgat
     4621 gacttcgcga gatatgtggg catggatgcg cgcgacgtga agcggctggc agaccgcggc
     4681 aagctgccag ggcagaagat cggcggggaa tggcgattca accgggcacg ggtcacggaa
     4741 tggctccagc aggaaatgca tacgcttggc gaggagcggc tgatcgccct tgagcgaggc
     4801 atccgatcga gtacggggca gtcgtcggca accgacgagt tgacagtgac cgacatgatc
     4861 ggcctcgaag ggattgaccc ctcactgccg gcccggacac gagcctccgt cttgcgtgag
     4921 ctggtgaacc tggccgaccg cacgggtctg ctgtatgatc cgacgggtct tttgagtgcc
     4981 cttgagcaac gtgaggagat gtgctcgact gccctgccca acggggtggc attacctcac
     5041 ccgcgacagc ctatgccgta tgtctcggcg gaaccgctga tttgtgtcgg ccgtgtgcca
     5101 aagggcatcg gcttcggatc gacatcgcgc gagctgacac atctcttctt cctcatctgt
     5161 tgtcaccagg atcaccatca cctgcgggtt ctggcgcggc tggttcgaat tctcgtacct
     5221 gagacgatct caaccctgct tgcggttgaa acacgcgagg agatgcttca gatcctgatc
     5281 gcggccgaaa aggaaatcgt gaccgagaga taggcaatca ggcagcattc ccgggaggcg
     5341 gaggcggacc cggtttgggc gtactgagca tccggcaagg gtcaggtcgt tcgggtgggc
     5401 gccatgtgcg acgcgagctg gggagccgag gagtttatca gcgagccgat catccctgcc
     5461 tcgggaaccg gcgacataat ggccatggcc cgcagtgagc cgggtctgcc ggcgcggttc
     5521 acatggcggc agcgggagta tgtcatcacc gccgtgctga aggcctggaa aaccagctct
     5581 ccggaaggtg ggaccggccg tctgtatctg cgcaagcact ggtacaccgt gcagaccgat
     5641 gttggaccgc gaatgacgct ctactgcgaa cgtcagaccc gcaatcgacg gaatcccaag
     5701 gcccgctggt ggctctatag tgtccagggg catacccgct aagcggcgca tcgggctttg
     5761 cttgcccctt tgcggctcgc ggcgtatcca actataacac aggccatctc gacgtttgga
     5821 ggagcctgac ccacatggca cgcgctgacg gaacgtcccg cccgcatacg gcggccggct
     5881 ggctggcctc gctgttggtc tttttcatcg gcgcggtcta cgtctgcacg tggtggggtc
     5941 ttgggattgc gtgggcccgc ctgtggttgc ctcctatact ggcccggtcc tggggcggca
     6001 tttcccgtgc agtgaggcgg tgggagggcg actcattctg gcgatggctt ctgacgcaat
     6061 acatgattgc gcttgtgccg gttgtgatct gcgcggtcct cggacgcaaa ccgcgcgact
     6121 tgggcttggg ccgcttcaac gttctcgggt ggcggctgat cgcagtcagt gtggtcctgt
     6181 cgattccgtt cggtttgcta attatctgga gcgatcctgg ctccataccg ctaccgcaga
     6241 cctggtccga agcagcgtct tattcgggac ggctcctggc cattgtgccg gagcacgtgc
     6301 tggtttgcgg attgttcgtt gcactcatgc ttccggaccg tcgcttgccg gctcgcgtgc
     6361 cggtggcggc ggtcaatggt gcctggcggc ggcgagcatt gcgatggttg gggcttgccc
     6421 agcctgtcga gggcagccgt gtactggcat ggttcgggct gaccggaacg tcactggtgg
     6481 cggtagtgac atccggcatc ctcttctggc ttgctcacct gggcaagccc aacatcatgg
     6541 aggtgagcct ttcgctgccg ggcggtgtcg ccgttgcgta cgtcacgctg cggacgggct
     6601 cggtctggcc ggcgatcatc gcccacttta cgatgaatct gatcccgggg ggtattgcca
     6661 tgctcttcgg gtaaccggcc gcgtaggccg atagcggtca gaagttgacg actgggggaa
     6721 tatcaatgca catagtgaat cccaggccac gtttcgaaac cagcccggaa gaaacctggc
     6781 gtatttttcg catcatggcc gaattcgttg aaggattcga cgtcatggcg cgcatcggtc
     6841 cggcggtcac cgtcttcggc tcggccagga ccaagcgcgg ggcgcggacc tacaagctgg
     6901 gcgtcgatgt aggtcggcga ctggcggagg cagggtttgc ggtgatcacc ggaggtggcg
     6961 gcggcatcat ggaagcggcc caccgcggcg cggccaaggt gggcgggacg accgtcggcc
     7021 tcaacgtggt gcttccccac gagcagaaac ctaaccccta catcgagata gaggtcgatt
     7081 tcgattattt tttcgttcgc aaagtgatgt tcgtgaaata cgcggtggcc atggtctgcc
     7141 tgccgggcgg tttcggcacg ctcgacgagt tcttcgagag catgacgctc ctgcaaacgc
     7201 agaagactcc gccatctccg gtaatactga tgggcaggga ctactggaga ccgctgctca
     7261 agtgggtcag gacgacggtg ctgaataagt atcaggcgat caatcccgag gatatgaacc
     7321 tgtttaccct cacggatgac ccggccgagg ccgtcgatat cattgtgaag aaataccgtc
     7381 aaacgggtac cctgtgggaa cacccggtgc gcagtcatcg acggcgagca cccacgaagc
     7441 cgaaggattg aggtcattcc cggcgaatga gttcgctttg cggcctgttc ccgagcgcgg
     7501 attcgatcaa cgaccgcgcc gatcggccgt ggcgattccc ggccgtcgcg cggaaggctt
     7561 ggattgccga cccatccccc gacttatcga taccctccgt cgctcgcccg cgtttgcgtg
     7621 cagaatgggt atcccgaaaa gccggaatct gccggacgtc cgaaaacacg gtgagatgat
     7681 tggagctgtg ccctgggatt gggcgtaaca ggactcgaac ctgtgacctt acgggtgtga
     7741 tccgtacgct ctagccaact gagctatacg ccctcaatac cggttattgt accatggtcc
     7801 cgggtggagg caaggttcgg aataaggcct gcgagactct ggtggtcgtg gcgaaaactc
     7861 acagccgccc ttctgcgaga gccttgagag accttgactt cgggacacga cgacgtgcac
     7921 aatactcctc gtgccgaagc ttgcgcctcg ggagcggtcc ggtaccctgg gcgggcagcg
     7981 aggaaggcaa cctggaaccc tggccgaggg cgggccgcgc cggaatcagg tagctcgacc
     8041 ttgcgagacg gcctttgctg ctagagggcg gcgagatgac atcgcttgtg gccagtgcgg
     8101 cgctattggc cgcctggtgg gtcaggctcc ggcgcggacc atgctgaatg aatgagcgcg
     8161 agtttggaga actcgcactg aggcgtcgtc ctgaggggaa ttgcccagag agaaaaaacg
     8221 ccaatgggac ggaaaggaca caagtccggg cgaaacccta tcattgagta ccgacggctc
     8281 tccacggacg atcttcgacg cagaattgag cagtgccgcg agaaaggcga cactcgaacc
     8341 gccatcgaca tggccaaaga atgccatcgt cggcagccaa gcccggaaaa ctccgatctg
     8401 ctcggtcatc tctaccttga gcgcggtcgc cggctgatcg agaagaacct gttcgccgag
     8461 gcactgatgg tcctcaataa cgccttgagc ctcggccagg gcagtgcgga cctcttgtgg
     8521 ttgatgttcg agtgcggcct tcgcagccgg caatatgaga cggctctgtc ggcgttgagg
     8581 cggctgaacg acccggccga gcaatcgcga gcttcgcgat acctggccga cgaggcgatt
     8641 gcacgaggcg aatcgttgga ccggttttgc gaaccggcga cgcgcgagga tgtgcacaga
     8701 atacggcggg ctttcgcggc ctttgagaaa ggagacgacc cggcggtcac ggatgaactc
     8761 agggggatcg gcctgacgag cccgtgcgcg ggatggaaat ggctgcttct cggtctggcg
     8821 gcgtacactc gaggtgaccg cgaccgtgcc cgcctgagtt gggacaaatg tgggaccgag
     8881 gggcggccgg gacgcctggc ggcaattctc cgatcaggcc tgttgaacga cggtcgggac
     8941 ggcgagctga caccggcgct tcgcgagagg ctcatagagc attttggcag tcctcgcctc
     9001 gccctcttga cgcaaataaa ggagcgtctg gccggcaagg acgtgagggg cgccttggac
     9061 ggatgcgccc gtctcctggc gagggtggaa gcgagcgaac gcgaggcctt tgcccaacgg
     9121 cttggccgcg cgatctgcga gaagatgggt aacgatgaag ataccgtcca acgcttcagg
     9181 cgagtgttgg gaccgctccc ggaagaccca tttctctatc ggtcggcggc gctgtctctc
     9241 gaaggctctt tcccctcgga cgccatcgac gtctggtcct gcttcatcga tacattagag
     9301 catgtgaaag gaatcgcacc gcacctgttc ccccgggcca agggccttgt gctgcagcgg
     9361 atgggtgacc tggcgatgca agcgagcaag gtcgacggct tcgaagcatt tctcggcttg
     9421 gcctcaggcc gctgcaatga ggcgatcggc tactacaggc aaagcctcca ggagtatgcg
     9481 gaggataaga ccagccacga aaagctgatg gccgcgtacc tgttgcagaa ccagcccaag
     9541 aaagccgagc ggcacgctcg cgaggttctg cgccgatggc caaacgatgt taacacccta
     9601 ttgttcctgg gactccgttg tatcgacaga gctgcgtttc gcaaggcgtt agactacctg
     9661 aagcaggctc tgcgagtcga tcccgccgac agtcaggttc gtgcgggcat agtgttgtgt
     9721 tatctcaagt cggcccggcg gcgattgcgg caaggaaacc tggaccaggc tcgccaggat
     9781 tatgaggaag cccaagcggt gctcgatccc acggcatcca aggcactctt tttctgcaga
     9841 tgggcggcgc tggaatggcg ggccgagaag ccggatcggg cgcaagagtt gctgagccag
     9901 gccctggccg gtcgcgagga gcgggtggtg gtctatttcg ttctggcaat cgaactgtcc
     9961 gttgcgcaag ccccgccgga ggtgcagcag agatttgaca agttgctcga agaggccttg
    10021 gccgatgagc caagcggtgc ggctgcagcc cggatggccg agtacgtgct tcagatggcc
    10081 gaagagggcg tggattaccc gaaatcgcgg atacatgccg acctgcttgt cggctatgtc
    10141 tcgcgagccg cgggacggtc gaccttctcg gaactgcagt tgctgcaagt ctgcgaatac
    10201 ctcgaagatc ggcaggagtg gaaactgttg gagcgtctgg cgcaccaagg ctgcgaccgg
    10261 ttcgtcggaa accctcactt tccggtgttt ctgggccgct cgcagcgcgg cctcggccgt
    10321 cgacggttgc cggcccgtac gcgacggcta ctggaacgcg cctacgagca agcacaggcg
    10381 caacaccggt tcgatctctc ggccgagata tcgtcgctgc tggatgagcc gcaaccctcg
    10441 atgtcgcacg gcttcggcga tttcaggtca ctgttcaatg cgctggcgca catgcttgat
    10501 gcaagcgagg ctgaggatga taccggtcga aaaccttcga cctctcggaa gaaacctcgg
    10561 agcaaaggca tcgacatgca aaggatattc gaatggatgc aagagggaat ggatgaggag
    10621 aactaaggcg gaccgccttt ttgcgggccg caacccaagc gctctgcgat cgagaaacat
    10681 gctcgcaaaa tgcgaaccag tcgcgacgga agagactaat ggcgaatccg tacagcatcc
    10741 tgaatgtcag gccggaggcc ggcgatgaag agatccggcg gtgttacttg gaagcggtgc
    10801 accggtttcc gccggaacac tgccccgagc agtttgtatc cattcggcag gcatacgaga
    10861 ccatcaaaga tgaaggcgcc cgactggagt ttcttctgtt tgacgcctca caaggagaat
    10921 cgatcgacga gctgatcgag gaggaaagat gtcggacaac ccccaaacgt gcgggtctgg
    10981 cggcattatt gagccttctg ggcgagacac gatagtcccg ccggaggcgc ggcaggaaat
    11041 cgaggcggtg ctctcgacgt tgcgccagtg gctgctggag tcggaacagt ggcgtcatgc
    11101 gctcctggcc ccgcaagaaa acgttgatgc ggcagcgcgg gtcgatttgc acacgttgct
    11161 gagcgaactg atcgcgctga agcaggaagt ccggctggag gcacgaggca ccaagaccgc
    11221 gcgtcaggac cttgatcgag ccatcgacga gtttcatgag ggcatcgaag aggtttcggc
    11281 acaaacccag aagctgttcg atcccctgct gcgcgagcgg gatcgcctgc gggacggttt
    11341 gaactcccag atggattcac agctacggtc ctgggtggag gtgctattgg atctgcgcga
    11401 agccctgtcg cggggcgaag agacctcccg tcaggcggcc cggcgtgtcg gatggcgtcg
    11461 ttggctgctg ccccgaggcc ttctggaagg tctccacgaa ggatacatgc tggggctgcg
    11521 tcgcatagat gcgatcctgg aagcccgcgg ggtccgtcct atcgagtgtc tcggcaaacc
    11581 cgttgatccg gaacagatgc gggtggtcga tctggtccgg cgcgacgatc tgccggcggg
    11641 ccacgtagcg gaggtggtac gcgccggcta cacctgcgat tcgaaggtca tccgctatgc
    11701 ggaagtccgg gcggttgccc gccaagaagg agagacagca tgagctcgga accgatcatc
    11761 ggcattgatt tggggaccac caattcggag gtggcggttc tcgaaaacgg ccgccctcgc
    11821 gtgctgaccg aaaacggaca ggccattctt ccctcgatgg tcggcctggc cgacgatggg
    11881 cggctgttgg tcggtgagcc ggcgagaaac cagtggctgc tggctcccga tcgaacgatc
    11941 cgttcggtca agcggcgcat gggcgaggac gtgaaactgc gcatggggga ccaggagttc
    12001 tctccgcagg aagtgtctgc gatcattctg cgaacgctca agaaccgggc ggaacgtcac
    12061 cttggccgcc cggtcggcaa ggcagtgata accgtccccg cgtacttcaa cgacgcccag
    12121 cgtcaggcga cccgtgaggc cggcgagttg gccggtttgg aggtcgtgcg gatcctgaac
    12181 gagcccactg cggcgtcgct ggtctatgag gctggacaat ccgcgccgca gaaggtcatg
    12241 gtgtacgatc tcggcggcgg aacgttcgac gtaagcatcg tgaggatcga acaaggcgtg
    12301 atcgaggtgc tgtccagcca cggagacacg cacttgggag gcgacgactt tgatgccctg
    12361 ctcatcgagc acgtggccgg ccgcttccaa gagcaacacg gcgtcgacct ccgggccgac
    12421 ccccgggctc attcgcgatt gtggcgggcg gtggaacagg ccaagattct cttgtcaacc
    12481 acgccatacg ctcaggtcaa cgaggagttc atctgtgaga aagacgggat tcctcttaac
    12541 ctgtcgatgg aattgagccg aggcgactac gaggaaatga tccgtccgct gatcgacaag
    12601 accatggaag cggtacaggt ggccttgaag gacgccggcc tgactgcggg ccaaatcgac
    12661 cgaatcgttc tggccggcgg ggccacacga acgcctcttg tcagcgagga attgcggaat
    12721 cgcaccggcc gacatcccca ccaggaggtg gaccccgatc tctgcgttgc catgggagcg
    12781 gcgatccagg caggcatcat ccaaggcgag agcgtctccg ccgtgctggt tgacgtaacg
    12841 ccccacacct tcggcatcaa ttgcctgggt gagcgtttcg gcctgcctta tccctactgc
    12901 ttcagtccga tcatccataa aaacacaccg ttgccggcat cacgaacgga ggtgtactac
    12961 acggtctgcg aggaccagga gcgagcggtc attgaggttt ttcaaggtga acatcccgac
    13021 gcgctcaaga acgtgcgcat cggtcggttc acgatcgagg gcttgagcaa ggggccgcct
    13081 cagagcccga ttacctgccg gctggacctt gatttgaacg ggatgctcaa ggtgaccgct
    13141 caggaaaaga agacggggcg gcaaaagcag attgtcattg acaatgcgat gagccgtttc
    13201 gagaaaacag aaatggccga tgcggcccga aggctgcggg ttctgttcgg agatgacgaa
    13261 caagaacagg catccgcgcc cgaggccgat gccgtttcgg aggcggcgga atccgatcac
    13321 ccggcacggg aggtcgtgcg gcgggcacat gcggcgctca aggacgtggc cggcaaggat
    13381 cgtgaagagg tcctgggact gcttcagcgg attcacgatg ccatagagaa gaatgaccgg
    13441 gaagatgcag atcgagccgc ccaggaactt gacagcattt tatactatct ggaggagtcg
    13501 aaatgaacga cctctccgga tcggatgcct ttgggaaagg tcgtgagcgc tgcccgacct
    13561 gcaatgcccc ccggcaggcg gaatcggtct gctatcgctg caagagcgac ctgtcgtcat
    13621 tgatcgttct ggagagccgc gtcgacaacc tgcgggcaca cgcccgtcgc tgttacgcca
    13681 gaggatggta tcgccgggcg gcggaactca cacgggaatt gctttccctg gaggcatgct
    13741 cagaagatct ggcgctgctg gcctgcgcca gcctgatgag cggcgatttc ccctcggcgg
    13801 tccatgccta tcgccggaac gtttttctct cacagggcaa cagcagttgc tccatgagcg
    13861 acacgtaagc atcgaggcac ttctgggcgc agcacatttg atcggcctgg cgcaaggcct
    13921 cctgagcgca gcggcggcgg gcctcggcat cctcccaggc cgtgcgaatc cgggtggcca
    13981 aggcgtgggg ttgcgaggaa cccaccagaa agcccgtctg cccgtcgcga atcagctctc
    14041 ggacgctcgg accgtcggtc gcaacaatcg gtatcccggc cgccattgcc caagccaacc
    14101 agccggttga atgactctgg tcatcgggcc atatgaacat gtcactggct gcgaggagat
    14161 cggccggccc gtattgctcg gctgcaattc ggaagatctg tgggcagtaa cactcctcgc
    14221 tgaggtgcct tgcccgcccg tcgccacgaa acccacctgg caggatcatt cggacatccg
    14281 gccagatctg gtgcagaatc gctgcagccc aaacggcagc atactgccct cctttccttc
    14341 cgggcgggca agcggtcagc aagactcgcg catccgccgg gaggcccatt tccgatctga
    14401 cacgttgccg atctgcatga cgaatctccg ccggatcgac gctgggcctg atgacccgca
    14461 cgctgtcact cttcaaaccc aagcgaatca gttgttctct gactgaatcg ctcagacata
    14521 caaaatttgt ctgctgagcg cgttttcgcc tcatcagatg cccgaaaaga atgccgggca
    14581 ttccgatagg gctggacagg atcgtcagcg acggctgatc gctgccctcg cggctgaagt
    14641 cgaggtatgg tgaccagtcc cacatcaaca gcagatcgaa ggacttccgc cgcaacagcc
    14701 ggacagcccg cgcacccgga agcatgggca tcaaggagca gccgtgcacg cgctcgatcg
    14761 gcaccgccgg cggtaggcgc agcaccgggg ccggtcgtcc gatcatgaga acagcttgcg
    14821 agaaacgctc gggcggcaac cggttcagga ggagagaaag gtgcgcaagg gcgtcctcag
    14881 aggtcgactc gtgcacagca tggagcaagc gggcggccat gacctcatca tgctcgaggt
    14941 ctggcccgcc ttcaagttga ggacaccccg ccgttttgcg agcgttggcg gagacggtac
    15001 actctggctc gtccttctga caccccatcg gccggcggtg ttatccgggc gggcacggag
    15061 gttatgggtt tcaaaattct actgcttctg attctgctgc tggcatcagc gttcttcagc
    15121 ggggccgaaa cagccctgtt ctcgctgagt cgccatgaat tgatgcggtt ccgccaggat
    15181 aaacggccgt cgaggcagat ggtcgccgac ctgatgcgtc atccgcgccg gctgctgctg
    15241 acgctgatga tcggcaacgt gtcgatcaac ctgttcattt tcgccgccag tctgtcgctg
    15301 tcgcagtcag tgctcggcag aaattcggcc ctggtcccga tcatcgggtt ggcttcgcct
    15361 atcctggtca cgttgttcgg agaaatcctg ccgaagggag ccgcgattct gctgagggtt
    15421 ccgctggccc ctcgtctggc tcccatggtc cggtttaccc aggtcattct gaccccgttt
    15481 cgtgcgttgc tcggaacgct gctggtcgaa ccgctgactc gtctcttggt cggcacaccc
    15541 aagcctgacg aatacgtcac cgttgaggag ctgcgcgaac tggtcgagat gtcggagcgg
    15601 caccgcgtga tcgacgctca cgaaaacgcc atgctcgacc aggtcattca gctcggcgag
    15661 ttgcggacca aggacatcat gattccccgg gtggacatga tcgcctatga cgttcacgga
    15721 aacccgcatg acctcgaacg gctgatgcgg aagcatgaat tcaccaagat accggtttac
    15781 gacaaggaca tcgatcggat tgccggaatc atctatgcca aggatctgct gctgcacccc
    15841 ggccgtccgc tcgcacaact gatccggccg gcccattatg tccccgaagt ggtcaccatc
    15901 acccaattga tcgaggagtt ccgccggaag aagacgcagc tcgccattgc ggttgacgag
    15961 ttcggcggcg tggtgggtct ggtcacggtg gacgacatcg cgaagctgat tgtgggcggg
    16021 ttgggtactg agggggaatc ggagcaggac agactctggg tccggcttga tgagcgacgt
    16081 taccgcgtct ccggcgggat gagcatccat gactggtccg agcagttcaa gatgagccgg
    16141 ctggacgagg aggtaaccac cctggcgggt ctgattctcg ctcggctggg ccggctgccg
    16201 gccgtcgggg acaagctccg gctgggtaac ctagtcctga cggttgaggc acttcgggga
    16261 cgacgtatcg aatgggtttt gctggaactc gtcgacggtg gatcgagtgc cgcgcctgtc
    16321 gatgggaagc ccggaggaga ctgcccatga tcgcgctcct gctgggcaca tttgcaaccg
    16381 cagtcactct ggccatatca ggcttttttt ccggggttga aatgggcctt tactgcgtca
    16441 atcgagtccg cctgaggctt cgcgccgaac gcctcgaagc actcgatgcc cgcgcgctga
    16501 tcgatctcac cgaccgccag gacgaaagca ttctcggtat cgttctcggc aacaatctcg
    16561 cgaattatct gctgacagtc gcggccacgt ttctgatggc ctgcggcatt ggcgtcgagt
    16621 cctcgaaggt ggagttctac acggccgcca tgctctcccc gctgggattc gtcttcgccg
    16681 acgtcgtccc gaagaactgg ttccgagcgg aagccgatcg gctgatgtac cgttgcgccc
    16741 ggccgctgcg agccatgatc ctggtgctcc gtcagacggg catcctctgg cttctcgggc
    16801 aggtcacacg gttcagcata tggctgtccg gccatgaagc agagagccgg atgcacggcc
    16861 cacgacgtga ggtgctcggc ctcttgcggg aaggcgccgc ggagggagct ctgacgaccg
    16921 aacaggcgga aatcgtcgag cgagtgatga atctgtcgag cgtccgcgtc ggttcgatca
    16981 tgattccgcg ccgccgcgtg gccagcgtcc ccatcaccgc aaaccgcagt caattcctcc
    17041 gggcggttcg gagccacagc tactcgcgga tgccggtcgt cgaccgagac gggaagacgg
    17101 tctccggcat cgtcagcgtc tgcgacgtgc tcgccgacga taccgatgcg agtatcagca
    17161 acttcatgca cccccccatt gcgctccagg ctcaggactc cgccgcgcgg gccatcattc
    17221 agctccagcg agccaacgag accatggccg tggttaacga tccgcgacga ggcttcgtgg
    17281 gcatcatcac actcaaagat gtaatcgagg aaatctttgg cgaattgccc gcgtgaaaca
    17341 tctgatcaag gcagcgcctg ccaagagagt cctgcctgca gcgttctggc tctctctcct
    17401 gtcagatgtg cgatttgtgc gcgcacgtgt ctgtggcacg gggcgcttca attgcggccc
    17461 gcaaaaaggc ggtcccctta gaatgtcacc gtcaggcctc cgtcgacgat catcacatga
    17521 ccggttatgt agcttgactc ccggcaggcg aggaacagga tggccgaggc gatttcctcc
    17581 ggccttcccg cacgggccat cgggattttg cgcgcttcca cgtagaacct cttgaaccag
    17641 tcgctttcca gctcgttcct gccgttgtag accgacatcg gcgtgtcaac aaagcccggc
    17701 gcgacggcgt tgacgcgaat gttgtgcgga gcgagttcca ccgacagggc tcggctgaac
    17761 tggttgaccg ccgccttggc ggccccatat cccgaagccc ccggctcggc ctgcgtgcca
    17821 tggatgctgg tcacgttgac caaccgtcca ggcgtcttgt ttgcgatcat caccctggcc
    17881 gcctcccgac agaaacgaaa cgcacccacc acgatgatgt cgaaaatgtc ccgccaggtc
    17941 tcaaagtcgg cgtcgggaaa ccccacaggg taggcgacgg cggcattgtt gacgaccaca
    18001 tgcagccctc cgagcttggt cgcggccgca atcgcggcgt cgaccgtctt ctggtcgcga
    18061 acatcgcccg gcacgacggc cagccggtcg gttcggcctg cggctctggc tgtttcctcc
    18121 agcttctcgc gtcgtctgcc gaccatcaca accgacgccc cttcttggac gaagcggaga
    18181 gccgttgcgg caccgattcc cgttcccgcc ccggttatga tcgcaacttc gccgtccaga
    18241 agacccatgt gggcagctcc tatctgacgc gcccgtctat gctgatatgc tcaatccggt
    18301 cgcgtcccgt gaatactacc gcggccgtct ccgtctccac gacctgcccg gagtccaggt
    18361 gaccacacct cccgctcgtg gaggcggtcg ccagatcacc aggaaagccg gtcgcggcct
    18421 cgggaatgac cgtgcagagg cctcgccatc ccccgtaggt ggcgaacaac catagagtat
    18481 tgaagagctg gttgtcaaaa gccagaccga aaccgctgcg cgtcgcgtgg tcaagatggg
    18541 cataccagcc ctcgtgcaat tcggtcaggt agaccatctc gaaatgcggc acatccaccc
    18601 ccgccgggac acggctcata tcgaacagct tgccgtcacg gccggggacc tgcggccagt
    18661 tgaactccaa cggttccgcg gccaaccggc ccagccccgg actgccaatg atgccctttc
    18721 cggcgggaat gacgaactct gactgcgggc cgacggacaa agccggatgg atcgaccaga
    18781 cgtagtcgaa accatgacga ccgaggtggc tcaagcgata cttcacgcgg acgacgggcg
    18841 atgatgccgc cagggtcatc catcgttcca ttcgcgtcgg cgtgacaccc ccttcggccc
    18901 agaggtgcaa tgtcagctcg gtccgacttt gcaggacttc ccactcgaaa ggtcgggtcc
    18961 agtattcgcc atgatcgggg tacatctcgt ggttgtaagg cccggcctcc ccgttgggga
    19021 acagctcgtc ccagccgccg ctgaaattgt cgtcgtagtt catcccggac ggcatgattc
    19081 tcggcatgat ccgaggatgc tgccacagga agtcgcggtc ggtcggcttg tggacccaat
    19141 gatagatctt gccgcccagt tgaggcagca catcaatccg cagcacgtcg ctctcgatcc
    19201 gcagaacgct gcattcgttg agcttccagt ccttctcgaa tcgaacatag cccacgagac
    19261 cgtctccttt tcgattggac ccgggatgat aacacggtta cacttccagc ggtataagca
    19321 ggtccgcccc atggagacgt gctgaatgct cgcaagcagt taccagcctg tcgattatgc
    19381 catcatcatt atctatttta tccttacgtt ggccatcggg ttgtggtcag caagaaaagc
    19441 caaaggggga tccaaagagt tcttcatcgc cgagcggtcg ctgccgtggt gggtggtcgg
    19501 cttcaccatg gtcgcggcaa gcatcagcgc tgagcaaatg ctgggcgagg tcggatacgg
    19561 cttcggggcc gggttggtgg tctcgaactg ggatctgggt gtgtaccctt ccctgacgct
    19621 gatgattttc gtgttcctgc cgctgtatct gcgcagcggc acgacgacga ttcccgaata
    19681 ccttgagaag cggtacaaca agacgacgcg tctgcttttt gccgtgtaca ccgtcttcaa
    19741 caacgcctgc gttacgctgg tcatggtgct ggcgctgggt gccacggcga tgaagtactt
    19801 cctgggaatc ccgccgattc tgggcacggc gatcctggcc gtgttcacgg gcatctacac
    19861 cgtttacggc ggcatgtcct cggtcgcatg gacggacacc tttcagtgca tcctgctgct
    19921 cgccggcggt ctcacgcttt tcggggcggg gctcaacgag gtacccggtg ggtggtccgg
    19981 gctgttggaa cgaatgcggg acgcaggctc ggcacatctc atccggccga tggatgaccc
    20041 ctacgtgcct tggcccggag tgatcctgct gatgttgtca accaacgtct ggtactgctg
    20101 cacgaatcag ttctacgttc agtcctgcct cggggccaaa gacgaacgcc acggtcgcat
    20161 gggtgtgctc ttgacggcct tccttgggcc ggttctgact ctttgcttcg cctttcccgg
    20221 ctacatcgca agtgaccttc tgaagcaggg cgtgctgccg gccctgccgc tcacaccgga
    20281 cggccagccc gacgccaatg ccacgtaccc gcatcttgcg cagttcctgc tgggtccggg
    20341 gcttcgaggc tttctggtcg ccgccgtcct gggggcgatc atgtcgagcg tgagcgccat
    20401 cgtgaacgcc accgccagcg tctacacgat cgacattcac cgacgctggc tgcgtccggc
    20461 cgcaagtgat cgcgaactgg ttcgcgtcgg cagactggtc ggtttggtta ccctgctcgt
    20521 cgcgttcccg ctgaccctgc tggcgagcag ctaccgctat atcttcacct actcgcaaaa
    20581 tgcctggtgt attctggcca tcccgatcat gctggcgttt acctatgggg cgttgtggaa
    20641 gcgcacgacc gccaaggcgg cgaccgcaac gtttgtcttg gccgcgcctt tcgtcgcagt
    20701 gccgttcgtc tggggcaatc ccgatgacaa ctggctgcga ctgccattca tctcaggcag
    20761 aattcacctg ttcaactttg ccttcgtgct ttggatcgcg gcggccgtct tcatgatcgc
    20821 ggtcagcctg ctgacgaggc cggcgccggc ggaggccgtc gccccgtatg tctggagacc
    20881 gtcgctcaac ctgccttcgc cggagcatcg cgggcagtat ccatggtatc agaccgtcgg
    20941 cttctgggcc gttatcgcgg caatcatgta tctgatcatc tatgtgatat tctggtgaag
    21001 accgatcatg tcaagactca tcgccctctt cgtgctattc atgccgctgt tcggttgtgt
    21061 ccggatgtcc ggttcagcat cgcaccgcgc gagcccgccc gtcttcccgg gcctgccgct
    21121 tctggtcgac gaggacttcg acaagccttc gacgcaatgg gagctggacg atccacgggc
    21181 gtggaagttc acgcgcgacg gcgacagaac ggtactatgt ctggccggga acagcagcta
    21241 caagccgccg taccggtcgc cgaccggaat ggccttgctc aaggacgtca tcgtgtccga
    21301 ttttgtcctt gacgcgcggg tgcgatcgac caaagctgat tatccgcacc gtgacatgtg
    21361 ccttttcttc ggctaccagg acgcggcgcg gttttactat gttcatttcg ggctgaagtc
    21421 ggacgacgcg tccaacacgg tccacatcgt gaacaacgcc gaccgtcgcc caatcgttaa
    21481 gacgcggaca gacggcacgc cgtggaccga gggctatcac cacgttcgcg tggtccgcaa
    21541 ggtcgccgcc ggcacgatcg aggttttctt cgacgacatg acgcaaccgt gcatgaccgc
    21601 tgaggacaag acattcacgt ggggccgaat cggggtgggg tctttcgatg atgcgggcaa
    21661 cgttgaccgg gtgatcctct ggggcaggaa ggtcagtctt ccggcgacac gaacggttgt
    21721 gccttgattg tgagaggaaa tcgcgacccg cacgggcgcg gcgcgatgcg cctacatcaa
    21781 ggccatgcgg agctgcgccc gtgcgccggc cacgatccgg ttcttgcgac gaagggccgc
    21841 gtcgtcaccg gccggcattt tcagggcttc ctcgaggcgg gcttgggcgt cggccgcgga
    21901 gatctcttcc ggacgcaggg ctttctgggt cagaacagtc acctggttct cgatcacctg
    21961 tgcaaaaccg ccgtccacaa accaagtttg cgtttccgtg cccttgcgca atcgcaggct
    22021 acccgcgccc agcttgcaca gaagtggagc gcggcagaac agcacgccga tttcgccgtc
    22081 atgggcaggc agcaccaccg actcgacgtc gccctcaaag acccgggcct cgggggtcaa
    22141 cacgctgcac ttgaaggtct tctctgccat cgcaataacc cgggcgaatt caaagctggg
    22201 aatcaggcat cgaggatttg agattccaca tttcagagct cacatttcaa atctcaaatc
    22261 cgaattctcg cattcgagat ctcaggaaca ctcgcaatcc cgattcttca cctcagattc
    22321 tccatcatcg cttcctcata ttcaactcgg tcctcaatcc accgttctca attctcaatt
    22381 cttcgccagt ttctccgcct tcgcggccgc gtcttctacg gtccccacgt acatgaacgc
    22441 ctgctcgggc aggtggtccc atttgcccgc gcaaatctct tcaaagctgc ggatcgtgtc
    22501 ttccttgcgc gtgtagttgc cgggataacc ggtgaacacc tccgccacga agaacggctg
    22561 agagaagaac ctctcgattt tcctcgcccg gctgaccagg agcttgtcct cctcggaaag
    22621 ctcctcgacg cccagaatcg cgatgatgtc ctggaggtcg cggtatcgct ggagaatctg
    22681 ctgcacttct cgggcgacct tgtagtgccg ctcgccaatg tactgggcgt caagaatacg
    22741 gctggatgag gcgagaggat cgatcgcggg atagatgccc ttctcgctga ttccgcgggc
    22801 cagaacgatg aatgcatcca ggtgcgtaaa cgtggtcgcc ggcgccgggt cagtcaggtc
    22861 atcggcgggc acgtaaatgg cctgcaccga agtgatcgcc cccttcttgg tcgaggtgat
    22921 ccgttcctgg agatcaccca tctcggtgcc gagggtcggt tggtagccga cagccgaagg
    22981 cattcggccc agcagggcgg aaacctccga gccggcctgg gagaatcgga agatgttgtc
    23041 cacaaaaagc atggtgtcgg ccccggactc atcgcggaac cactccgcca tggtcagggc
    23101 gctcagggcc gcccggaggc gtgcgcccgg aggctcgttc atctgtccga acaccatagc
    23161 ggtgtgatcc atgacctgtt tgacgttgcc ctgggcgtcc ttgaaggtcg cctcttgcat
    23221 ttcgagccag aggtcgttgc cttcgcgagt acgttctccg acaccggcaa acacggagta
    23281 acccgaatgc tcgcgggcga tgcgggcaat catttcctgc acgacgaccg tcttgccgag
    23341 gccggcaccg ccgaacaggc cgattttgcc gccgcggaca aagggggcca acaggtcgat
    23401 gaccttgatg ccggtttcca tcatctccga cttcggggtc aggtcggcga aatcgggcgg
    23461 tgcatggtgg atcggccggc gggactcggc cttcacctcg ccgcgcatgt cgatgggttc
    23521 gccgacgaga ttgaacacac ggccgagggt ttgcatgccc acgggcacgg tcaccggacc
    23581 gcctgtgtcc tcgatctgag caccccttac cagtccgtcc gtcgtgccca gagcaacggc
    23641 ccgcaccttg ccgccgccca agtgcagggc aacctcgcac cagagcgtct cgcgttcggt
    23701 catgcccagg accttacgtt caacgttcac cttcagggcg ttgtacaact tgggcatccg
    23761 gtcctcgtcg aactccgcat cgagcgtgga gccgatcacc tgcgtcactc gcccgaaatt
    23821 gcgaccattt gactttgcca taattgtcgt tctcgttcac aaccgcccaa tgggctatgc
    23881 gaaacaggtt tccgacctgt tactggccgg caagatgccg gccccacacc tcacactgcc
    23941 tcagggctgt ccagcctcgg gaaagacctt ggccagatcc gttgctctca tcttctcgtc
    24001 cggcacaccg agcgcccgca gcaagccgac agccatcagc acatcgccgg cgggcttcat
    24061 gtgcacgcca tcagtggtaa agaaccgctc ttgcgacccg gaatcctttt ttcgtttctc
    24121 gatcgccttg agaaacaagg catgcaaatc cacgtattgg cacttgttcc gctgggccac
    24181 cctcttgccg gcggccacat aagcggccag ccgcttgttg ccctccgaag ccgggtcttc
    24241 ctcaatcacc gtgggtgcca gcaccagcac gcgagcgcct gactcctgag ccatcttgac
    24301 catacgttcc aggttccgct cgtaggcttc gagcacccgc ggatcaggcc gatcggccat
    24361 tcgatgccag acatcattga tccccacgct gacggtcaca attgcggggc tgcggcgaac
    24421 aacgtctttc tcaaagcggg cgaccatgtc ctcggccttc tgaccgccga cgcccacatt
    24481 gacgatcggc ggaatcttca tccccgcgta ctgctgggcg aacaccgcgt ccatcgccct
    24541 caggtagccg cccgcctggg tgatgctgtc ccccatggcg acgatctgtt ggccgggccc
    24601 gatcttcatc tcggccggac gggttgccgg ctgggtcgcg ggcggcggcg caagcgaaag
    24661 aatcatcgaa aacaatcgta tcggctccat tacctgagtg cctccgcgcc gctgacgatg
    24721 tccagcaatt ccagcgtaat ctgcgattgc cgggcccggt tgtagtgctg ggtcagcgtt
    24781 cggatcatgt ctccggccgc atcggtcgcg gacttcatcg cgaccatgcg ggccacctgc
    24841 tcgctcaccg ccgcgtcgat gaaacactgg aataaccgga ctctcacggt ttccggcaga
    24901 agttcggcca gcagttcctc gggcggcggc gtaaaatcat attgcacggg ggcggcggcc
    24961 tcgcctgttt cggtatccgg ctccggcggc tcgatcggca gcaactgcat gacgtgcgga
    25021 cgctgcacgc ctgccgagaa aaaccgcatg tatgccacgt ggacggcgtc gatttcctgg
    25081 cgctcgtagg cagccattac gtccgcggcg atcggctcga tctcgacgta ctgcggcttg
    25141 tcttcaaatc gcgtgtcggt cgccgccatg gcacgcccga ggaacttgaa gtacgagatg
    25201 cctttcttgc ccgtcacatg gatatcgacg gtctcgcctg cctgttcccg gactttcagg
    25261 tgctcgatcg ccgaacgcag gattccgccg ttgtagccgc cgcacaagcc gcggttactc
    25321 gtgatcacga tcaaacgcga ccgctttgcc tgatcgttca ccttcagcag gggatgagac
    25381 aggtcgccct gccctcgcga gagccggcgc accaggtcgg tgatccgctc ggtgtaaggc
    25441 cgggtcttgg tggcacggtt gaaagcggcc tgaaagcgtg cggttgcgat caactgcatg
    25501 gtccgcgtga tcttgcggat gttgatcacc gccttacggc gtttgatgat cgcccgtgcc
    25561 ttggccatcc gatcgtcctc tgccccttgg ccccgtccga ttggcgtgtt ttacttgctc
    25621 gccatgaagc gtttcttgaa atcgcttatg atgccgacca acctgtcccg gagttcgtcc
    25681 gtcaggtcgc cgctttgtat cagctgctcg cgaacctccg ggtactcgtc accaacgtgc
    25741 ttgagcaagg cggcttcaaa cgcggagatc cggctgagcg gcacgtcatc gcagaagcct
    25801 tgcgttccgg cgaagatgct gatcacctgg tcggccacgt gcatgggcgc atactgcccc
    25861 tgcttgagca gttcgaccat cgcccggccg cggtccagtt gccgctgcgt cgcggcgtcg
    25921 aggtcggtgc cgagctgggc gaaggcttcg agctcccgga aggcggccaa gtcaagccgc
    25981 aacgatccgg cgatcttctt catcgccttg atctgggctt tgccgccgac gcgcgacacg
    26041 ctgatgccga cattgatcgc cggccggacg ccgccgaaga acaggtccgg ctcaagataa
    26101 atctggccgt cggtgattga aatcacgttc gtcgggatgt acgccgatac ctcgccttcc
    26161 agcgtctcga tgaccggcag ggccgtgagg gagcctccgg accccggaat cttgcggatt
    26221 tcgtagtccg tcctgccgag cgccttgaga tccctctctg cctccgctct tcccggcttg
    26281 cccacgtaaa ccttctcgtt gatcgccggc ttgtagttcg cgggggccga cttgggaacc
    26341 agcacatagc gcgtcgcaag cttgcacgat cgttcgagca gccggccgtg cagatagaac
    26401 acgtcgcccg ggtaagcctc ccggccgggc ggccggcgaa gcagcagaga caactggcga
    26461 tacgccgcag cgtgcttggt gagatcatcg taaacgcaca aggtcgcctt gccctgctcg
    26521 tacatgaagt attcggccat ggcgcagccg gcataaggcg cgatgtactg aagcggagcc
    26581 gaatcggagc tgccggccga gacgacgatg gtgtaatcca tggccccgtg ctggcgaagg
    26641 gcatcgacca ccccggcgat ggtcgactcc ttctgcccga tcgcgacgta gacgcaaatc
    26701 accccggtgc ccttctggtt gatgatggta tccacgccga tggcggtctt gccggtcttg
    26761 cggtcgccga tgatcagctc acgctggccc cggccgatgg gaatcatgct gtcgatggcc
    26821 ttgatgccgg tctgaagagg ctcattgacc ggttcgcgtc cggcgatgcc cggggcattc
    26881 gattcgagcg ggcggcggag gggagtctcg atgacgccgg cgttgtccag cggccgaccc
    26941 agcggatcga ccacgcgacc gatcatcgcc tcaccaacgg gcaccgaaag cagttgtccc
    27001 gtgccgcgga cggtatcgcc ttccataatg ccgaggtaat cgcccaggat caccgcgccg
    27061 atcgagtttt cttcgaggtt gaagacttga ccgatggccc cgttctggaa ttcgagcatt
    27121 tcgccggcca tcgcgttgga gagaccgtag atctgggcga tgccgtcgcc gacctgcaag
    27181 accttgccca cttcggcgac gtcgatctcg ctcttatact tcgcgatctc ctcccgcagg
    27241 accgacgcga tctcgtctac cttgaatttc atattcacac cgtttgcaat tgtcacgcct
    27301 caaggacgta tccacgtccg ccgtgaatct cctcagtcgc gcgctcgcgc atccgcctga
    27361 gcgttcggct gatccgcgaa gccacgcttg cgtcaacctg gatgtcaccg taatggatga
    27421 cgacaccgcc gatcagttcg ggcctcgtct gttcgatgag gatggcctct ttcccgattc
    27481 gggcgctcat ttcctcgcga atcgcttgcc tctcgtcatc cgcaagcggc atggccgtct
    27541 cgacgatgac ttcctgctga tgatgcttct cctccatgcg caattccacg catcggccca
    27601 ccttgcgcag gaggttcaag cgcccccggt tgttcatgac ctgcagaagg ttgaccagca
    27661 aatcgctcat gcgccccctg aagatgcgct cgatcgactc ccgtcgcggt tcatcgtcca
    27721 ccgagtccgc cgccaacatg cggcatagat cgatctctcg ctcgataaag cgcatgaact
    27781 cggccatttc gcgggcgatg gcctcctcct cgccgcgttc ggccgccgcc gtgagcagcg
    27841 cttcggcgta cacgtccgca agggccatgc tttcatcaaa ggtatgagcc atcttcattt
    27901 cacccggtga tgcctgttca gttgcgactt tcggcgcccc gctctttgag ctttgccaag
    27961 gcctcgccga cgagccgtcg gtgatcttcg ggggtcaact gccgcttgag caccgccccc
    28021 gccatctcca ttgccagctt tgcggattcc tcgtggaggt cccgcagggc ggtgtcgcgg
    28081 gcgatgccga tctcgcgctt ggcccgctcg atcagcgcct cggcgttgcg gcgggcttca
    28141 tcttcgatcc ttcgcttgac cacttccgcg tcgcgccgcc cctcttcgac aatggcactg
    28201 gcctcctgct tggcgttctc cagccttgcg gtcagctctt tcagccgcgt ctcggcctcc
    28261 tcgcgatcgc gcttggcgcc ggccagggaa tcgtgaataa acttctcccg atgctgaaga
    28321 ccggtcagca acggcttcca ggcaaacctg cccagaatga ccagcacgag cataaagatg
    28381 accagagacc agacaagatt ccccaggtct ccgccgaaaa ggttgggccg gccgtcctca
    28441 ctggctaaca gggtgccggc cgtcgcaaga tgaagtaccg caatcaccat gtaattgtgc
    28501 gctcgagctg tcatccgtct ccatctcccg catggccgtc tcgaaaccgc atgcaagaag
    28561 ccccgcgcgc cgctcacgcg cagacgcact cgcagggcaa gcggcgccga agccgttctc
    28621 gctcactctc aaaagaacag caccaacagg cagaccacca cggcaaacaa cgttgcacct
    28681 tcgaccatcg ccgcggtaat cagcgtcgtc aggttgatct ggctggcaac ctcgggttgc
    28741 cgggccatgg actcgacggc gctgccgccg atgcgggcaa tgcccgcggc gccgcctatg
    28801 atcaccaggc cggcaccgat ggccgcgccc agaaagctcg cgcccttatc ctggagcaac
    28861 aaaaaaccct tgccttcggc cgctttgtcc gatccggctg ccgcagcctc ggcggccatc
    28921 gccggtgccg ccaagaccat cactgccaac gccgccgtca tcatcactcg cttcatgtct
    28981 cgcattcctt tctcttgtga agccggtgga aacggctcct gccgctcgtc aatcctaacg
    29041 ccatcgcctc tcggcctcgc ggcccacggt tccactccgt gggcatggca gggtgacttc
    29101 tcaatcatcg acgcgccact tctcagtgac caggctccgc cctgacctcc tcgccttcct
    29161 gctcttcacc gtgttcggaa tgaatgttga ccgccaagcc gatgaacacc gcggtcagga
    29221 acgtaaagat aaaggcctgg agaaacgcca caaagatctc caggaacgtt atcgccacac
    29281 tcccaagcac cacaaacgga gccacaacca gcagggctaa cgcggcgctg gctgcgccgg
    29341 ccatcgcgat gaagctcagc aacacgccca ggagaatgtg cccggccagc atgttagcaa
    29401 agagacgaat ggcgagcgcc acacacttga ccagcaaccc gaatacttca agaaccgcga
    29461 tcagacagtt gatcggaaac ggccccatga agaagtgctt cacatacgcc atcccgtgaa
    29521 tccgcaaccc gttggcgatc accatcacga gtgtgcacgc ggcaagcgta cccgtggtgt
    29581 agatgttgcc cgtcgcggtt ccgtagatgc catgcccgaa aatcggcttg gtaatcggct
    29641 caaggggcaa gagacccagg agattgcagg tcagaatgaa gaaaaacgcc gtccacagat
    29701 agggaatgaa gcggtccgta tagggcccga gattcggttt cgccacaaac tcccgaagca
    29761 tttcgcagat cgtctcgacc acgtttcggc cgccgcgcgg cgtcatccgc gtgatttcgt
    29821 ctcccctctg cggtactcga accagtcgcg gcagcaggat gatcagcagc acggccgcca
    29881 cgatcatcat gatgatgtgg ttcgagatca atgtgaacgt gtgcaatggg ccatcgtgac
    29941 caaccctctt ccacgcgtaa tcgaccacgt gcgtcagagg atttgagccg gttgccagaa
    30001 gatccatcat catgcccgct gccccgtgac tctgttccac cgaagcaata cgacggtctc
    30061 aagcttgacc atcaccacgt acgcgatagc cacccacgtc aaaaacacct ttggttctgc
    30121 ccaccgacgc caagcgatca cgaccatcgc gcctacggtc agcaccagcc ggatcaggcc
    30181 gaccttcagt ccggcctgca gccgccctgc tgcgtcgtcc gaaaccatcc gccgcagcgc
    30241 cggcaagagc gtggcgtaca ccaccagcac tacaagcaat tgtccggcaa ccattccctc
    30301 catgcccgcc cgacccccga tgcgccatgt cggatagcag cctgcggcag ccagaacgac
    30361 tgtcgccatc ccgggcaggc agagcaacca cggaaccttg gatgtcgata ccacagacga
    30421 gctcaccacg atgtcgtccc gtcaggcccc acgtctttgc gattctttct ttctcgtgct
    30481 cgtggcggtt ccgccatctc atgcgtggcg cgccggatca gattgtagag gcctcccact
    30541 atccccacga tcgcgcatac cagcaggccc cagggtctcg tgccgaagtg gcggtcgatc
    30601 caccaaccca acaggcaagc cccacccaca cccgccgcca gttcgacacc cgcgccggcc
    30661 agttgcatta tgcctggcgg caaccgccaa ggtgacttcg ggtcgctcat cgcgctctcc
    30721 gaaagcggcc gtcctgtcga ttcccttcct gccagatgaa cgtttccgca cagacaagct
    30781 cgtgcagcca cctgcctgcg gtcgaggcga gtcgccctgc atggttacct tgccgcgccc
    30841 ggtccgcgcc gagacccagc cgcgcagcgg atcggaagtt taggctcgct catcgcgagg
    30901 gtcaagccgc agaccgaaag ctcactcgac gtaacactct atcggcacgg gaagtgcaaa
    30961 aagctgaccg gcgggcttcc gctctccggg atccggtgtg ttgctcaaca agggcgaacc
    31021 ggcaaggtta gtacggcagg ctgattgccg acgttacagt ctcatgactg agacatccga
    31081 gacggccggc cgtctgatcg aatcgtcgga aaacgaacag gcctcagccc cactgccttg
    31141 gttccgaagt cggccgtgag gctgggcctg tctcactgcc ccccatgcgc ccgtccgtgg
    31201 acggtccgat ccaggcctcc tccatgaagc cggcgcggac gtctacgcct ccaactgcgc
    31261 cagcaaaggg ggacgacgac ggcgacggcg cttcttggga gcgccgggaa ttcgaatctc
    31321 aggtttgcgc ggttgcgcgc ttgctgcaat cttcaaggcc tcggcggcat tgggcgcaaa
    31381 caagtcggcg ttgacctcct tccacaaccc ctccgcacga ttgaacacgc cgccggaaat
    31441 catgatgttc atcgtcggat tgctgccgac ctcgcgaatc agatcaataa gccgccgtac
    31501 gccgggaaca ccgccgggct gcgtgccaaa gatcatcagg atatccgggc gcagccgccc
    31561 caccagttcc aggatctcat catcgggcac ccctccgccc acaaagtaca cgtcccagcc
    31621 atccgcctcg aaaaggtcgg cgcacatctg ggcgcccagc tcctcgggtt ctccttccgc
    31681 acaggtcaac agcatgcgtt tgccgtttgc ctcgcttcgc ggcagtcttg cctgtaactg
    31741 gtcagcgagt gaacggctga ttcgtacagc catgtgctca gcggccaggc tgatccggtc
    31801 ctcgcggtac atctgcgcta cgtgctccat cgccggccag atcacacttc ggtagacctg
    31861 cctcgcttca agcccttcgt tcatcgcctc ttcgagcaca tccctgcact cctgacgtcg
    31921 ccctgccagt agaggtatca tcaacctttg gaaatgacgc tctgcactca tcatcacgct
    31981 ccaggctctt cggccaacga tcggcgcagg gaatctccgc accgcctcac cacaccgggt
    32041 tttctgatgt tcccacccgt cgcatcatgc gttagatgga aagtcttccg ctagggaagc
    32101 ctgacatgcc ggctgctccg cgccagcaac gatgaaatgt cgcctctcac cttgccccaa
    32161 ttttctgccc ctactcagaa cctcaccaag tctaccctgt atgtcaatct gggtaatctg
    32221 ggaatcatgg cgagactttc atcggcggga cctctgaaaa aacttgaacg gccctgcgta
    32281 acttgcgcta aagcccccca ccgaagagaa agacggacga cttgcgtccc ataaaaacgg
    32341 ccattcaggc tgctgacaag gaaccgcagc ttaggtcgcg ctccagcagg caaaaatcgt
    32401 ccttccccga agcacagaat gcagtgtcga aaacccggct ggatcgcgcc gcataccaat
    32461 cctttttcgg cgccgaaggg agggaatgcc ttgttgaggc gtcactcctc ccccacctca
    32521 gtgccttgtc cgacagtctt gcagatttca cgcagcaccg tcgtcgcgta gcaaccggct
    32581 tcgagccgca tgctcagcag gatgtatggt ccagcatcat cttgtcctgc atcgacctcg
    32641 acgtcctgcg gctggaaacg cagcgggcgc cgaccacctt tgacgcgatg cctgccggcc
    32701 tcgcgccatt catccggttt cgtgccctcc gcgttaatga gagccgtttc cacttgcccg
    32761 ggctcgccgt gggggtaagt catgcgataa ccaaagagcg ggccagttgg agagatctcg
    32821 aaggcatcgc aacggggttg ctcgccggcg acgtcctcga cccggaagac ggcgccttgc
    32881 ggatgccgcc aggccagatc accgaccatg agccgatcga gaccaccgat ccgcccggcc
    32941 acaatctggt tgaacaattg agactggtac gccgaaatgt agaaccgctg gagccgcatg
    33001 tcgatggaac ggattgcctt gcgagcgttg ccgttgaact tgatcaacat ccggcagacg
    33061 cgacgctcgt tattgaacgg gtagggccag gcatttgccg ctccctgcag gtctccctgg
    33121 tcgaagagtt cacgggcccg acgaacgtcg ccgtaatcag aaggcttcac tcgcccgaga
    33181 agcacctgaa ccatctcgtc gtaatcttcc cgcagaaggg cccggccgat ctgccacgtg
    33241 tctccgcgca ttccaaatcg ctgcgggccg aagtagttcg gtactccccg cttgctcaac
    33301 acttccatcg ttgcgcggac atcaccgacg cgagccgggt caacgtcgcg gatcctgatg
    33361 aggaagcgat tgccggccag gtgtccgagc ttcagtttgt tggtgtgccg cgagacgtcc
    33421 acaattcgca tgcgaggaat tgaaagggtc cgaatggcat caggatcggc atgctcaacg
    33481 ctgaacatct gcgttgtcac ggcatcggcg tctttgagac cggcgtagcc gacatcgcga
    33541 ggcggccgac caagcgcccg ggctatttcg gccaccgcgc gcatcgtgga caagccggtt
    33601 ttctcgaccc ggaaatagac atgcgtcccc tggcccgagg gcgggtaaag cggcacctcc
    33661 tcgacgacaa agtcttcggg acgcgtcttg atacgaccgc cgattcccgg cacgtccgca
    33721 gtcaggtacg gcaattcgtc cacgttcagc atgagggcct gccttatctc atggctttgg
    33781 tgcacagcct tttcagggag gaaaccaccg taacggctca ccagcggctt tccgaaacgt
    33841 tgatctggcc gcggttgagc ctgtccagaa acgaaaccag atgatactgc gtttcaatag
    33901 cgggaacggg tacgcctgcg gcggccgcct gacgcaccgg ttcgcccaga atggcttcga
    33961 cctcgagcgg ttgcctgttc tggtagtcga tcaacattga cggcctgtac ggcggcatgt
    34021 ccagagtgtt ggacatcatt ttctccgtca tgacgggaat atcgatggaa cagccgtggg
    34081 cgttgccggc tcggccggtc tcctgcatca gcgtccaggc aagttgcctg aggccggggt
    34141 cgttgacgat ctcgtccttg gtcactccgc cggcggtaac ggtcagcgta ctgaaaggga
    34201 tgttccacat cagcttctcc catcgggcct gtttcagaga aggccgcacc atggtttcga
    34261 tgcccgcctc ggcaaagagc ccggccatcg cttcaacccg ctcgaggtcg tc
//
LOCUS       DAOTPW010000023        32889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1426_length_32889_cov_5.444417, whole genome shotgun sequence.
ACCESSION   DAOTPW010000023 DAOTPW010000000
VERSION     DAOTPW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 32889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32889
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1426_length_32889_cov_5.444417"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="P5316_03485"
     CDS             complement(<1..505)
                     /locus_tag="P5316_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84049.1"
                     /translation="MKHIAAFVTLIVASTLQAASLIPVGVLDPAKPYSEIRALSADGV
                     YAVGCSYGTWVYPASSFTGNGPIIWSMSDGLVALPCPSGAATIAHGVAVGIGSNAGNI
                     IVSGLHEGNLVHRYYKAPQSNPGGGVWADTASAGGWSISSLCGGSWNDLRNAVPNDTG
                     WPPLGAWY"
     gene            complement(720..1964)
                     /locus_tag="P5316_03490"
     CDS             complement(720..1964)
                     /locus_tag="P5316_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84050.1"
                     /translation="MKVVKVLVVAVVLTAMGSTAVLGASLTSIGVLDPSAPYSLVQAV
                     SADGSYAVGTSKAVGGIQVPIVWSASDGLVALPNPSGQNSLAHGVAVGIGSNAGNIII
                     SGLHENNTTHRFYKAPLTNLAGGAWIDTATAGELGSAGNMRGGTSNDLRIQPGSDGRW
                     YTAGRRNDTGRNARYRGDPSSGWDGTAVRSVGSVSAYGVNVGRDNASPSNAFYEGPAV
                     SFGTVPGSTGSRADGIGISSGFGKSSTSDFDVQWICGQVQNYNGPGTTFQAFRWKRGD
                     ASMTFLGSLAPAGGGDTGNNSSCAYTVADNGVTAGHSYFGAVGTIPGYYVATVWDTSG
                     TWDTTGAPKSVKALLDAAGVDTSMWSSLTRVYAASDDGKVLAGYGVWAADGSTRGFVA
                     VIPEPATLALLTLTLPLLRRRR"
     gene            complement(2887..3540)
                     /locus_tag="P5316_03495"
     CDS             complement(2887..3540)
                     /locus_tag="P5316_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRR84051.1"
                     /translation="MMRGQTREAFFARIRESLTDRGAPVELPSDLEVARVTASGVDLV
                     TLFMDRAQQVGMRPERVVDDRAAAAKVVEIVTAAGRASAILPEEDIPARALIVDGLKG
                     KGVRLMDVNDRDAAFDADFGITGVSCAIAETGSLCVDSGGTHRRLASLAVPHHIALVR
                     AEQIVPDLLDFAARVSKDDIPANMTLISAPSKTADIEMILVEGVHGPKVVHIIVIAS"
     gene            complement(3537..4985)
                     /locus_tag="P5316_03500"
     CDS             complement(3537..4985)
                     /locus_tag="P5316_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRR84052.1"
                     /translation="MSGQTTQSHEFHPDFKKAAAAALADVRIGATFDRATRLNFDKRR
                     AIMPELGRPMAIRELAARIKDHTLQNLDRYLEQLVENVRKHGGQVHFAETGQQANEII
                     TGIARDVGASLIVKSKSMVSEEIELNQEIEKTGIEVVETDLGEYILQLAGEKPSHIVT
                     PVIHKTKEDIAALFRDKLGIEYTVDPEALTAAARKVLRAKFKAADMGIIGANFAVAET
                     GTICIVTNEGNGRFCSSRPRVVVCLMGIEKVVPRMKDLGIFLKLLGKSATGQRLTCYT
                     SLITGPKRPGDFDGPEEFHLVLLDRGRSRILAGEYRSVLRCIRCGACLNACPVYRRIG
                     GHAYESVYPGPIGKLITPLLASLEEYPDLPTASSLCGACLDACPVRIDIPTYLLHMRN
                     ELVEQGRMPWTWRIGFKFWRIGMSSAFLYRCGLKFGRMFMWLLSKDGWLKWLPMDGSG
                     WTDHRDFPLPAKKPFRARWADLQKQLDAEEGR"
     gene            complement(4982..5764)
                     /locus_tag="P5316_03505"
     CDS             complement(4982..5764)
                     /locus_tag="P5316_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRR84053.1"
                     /translation="MKVSLFVTCLTDTFYPDVAECVVRILRRLGHQVEFPEDQTCCGQ
                     PALNSGFFDEARAIAGRMIDVFRDAEVVVTPSGSCCSIVREYYPHLFKDDPVMYPRAV
                     AMAGKTFETVEFLQKKLKVDWSRWNLTYPAVATYHYSCHNRGIDMTPEDVEGLIRQFR
                     GLEYRRLDKIDQCCGFGGTFSVKQSELSGAIVWEKVKCIKATGAHMVICNDGGCTMNI
                     QGACRRAGLHVEFRHIMQILDEAMTASRPRMSAGQVTAEAAR"
     gene            complement(5794..6150)
                     /locus_tag="P5316_03510"
     CDS             complement(5794..6150)
                     /locus_tag="P5316_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84054.1"
                     /translation="MAKFSLRRYEDQARVSLAAGLASAVTFLVLLALVVRRFSVQELA
                     VFYGQRTYMAVVAAAAVTALLAVIGFGLGINSVGQRRNEKQVLSWAGFFIAAAVLVLT
                     ICVCIFFFLRGEFVGR"
     gene            complement(6526..7938)
                     /locus_tag="P5316_03515"
     CDS             complement(6526..7938)
                     /locus_tag="P5316_03515"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR84055.1"
                     /translation="MRTLRARLLIGTGVGATVVLLGSGILLYALVRNALWREFDESLV
                     AKARSLAAMTEQENGKIELDFDEANLPEFRPSDHAEFYQVRLPGGRTIARSASLEGRE
                     LIEIEGTVDRPAFESTRLPDGRRVRIAGIRFTPRQEDDGRRQQQPLVVSLVFGRQTKG
                     IETTLARIRFAMAAVGAAAMAVSLGVLAWFVRRGLRPLDRLANQIAGIGESELGVRIA
                     PAGVPAELLPVIDRLNGLLGRLERAFQRERRFTADVAHELRTPLAGVRSILEVALSRD
                     REPETYRNAMEDCLGINEHMQRMVENLLHLARADANQLEVAREGVDLAALIRECWKPL
                     AGQAEQRHLQVEWRLHEPRTILSDCGKLRLIVQNILSNAATYTNTGGWIRVEAICRDG
                     SVEFTVTNSGSMVAEQDVPRVFDRFWRGDAARQFSGGHCGLGLSLCKVLVELLDGSIE
                     AGSSPGETFTVTVRLPVSNA"
     gene            complement(7942..8610)
                     /locus_tag="P5316_03520"
     CDS             complement(7942..8610)
                     /locus_tag="P5316_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR84056.1"
                     /translation="MRVLLVEDYEPLRTSVSQGLREAGFAVDVSADGEEGLWYAESCD
                     YDVIILDIMLPVVDGLTILKRIRHSGRATPVLLLTAKDTVQDRVRGLDLGADDYLVKP
                     FAFEELLARVRALVRRKYAAKDPVLRIEDLEIDTAKRAVRRAGRQIDLTAREYALLEF
                     LALRAGQIVSRSDIWEHLYEFDASVESNVVNVYIGYLRRKIEQPGLPRLIHTRRGQGY
                     MLGR"
     gene            complement(8670..9401)
                     /locus_tag="P5316_03525"
     CDS             complement(8670..9401)
                     /locus_tag="P5316_03525"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HRR84057.1"
                     /translation="MKRALTLLGFVGLAIAVAVATAGDAPKTTLGQIPAEARATLRKL
                     ADGASITAVEREKENGMELYEAEWKVNGGKEVEAKVTAGGDLVEMEEEIDADALPTNV
                     KAVVAKHFPAGAKLECAKVTMVLYEIEAKINGKGKEILVSATGKIFGKDDDGDDDDDD
                     DDGEDEQKVSLDQVPAAVKATILAEAKGATVKEIERETKNGQTVYEAEWVENGQEVEI
                     KVASDGTLVKREVDQDDDADDDDDK"
     gene            10044..11627
                     /locus_tag="P5316_03530"
     CDS             10044..11627
                     /locus_tag="P5316_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRR84058.1"
                     /translation="MAAKPVAELIATKPQTHPCRDEAGVNPLAVAETARREVNASLDR
                     TRRSALGQFFTPPATAQFMASMCSVVQPQVHLLDAGAGLGALTAAWVNQICSRSSRPS
                     SVRLTAYEVDTTLLPALRQTMSACRQACEAAGIACEWVIHEADFIEAAVERLAPGLFR
                     AEDHPFTVAILNPPYKKFHADSRTRRLLRQMNIETTNLYAAFLALALLSLEVGGELVA
                     ITPRSFCNGPYFRPFRQHLFRHASLTHVHVFEARDHAFRDDEVLQENVIIRAVKGVSQ
                     QPTVSVSQSETPDDLIDTQREVGFDRVVRPGDHQAFIHLVPDGQGDHLADAMVSLPCT
                     LDDLGISVSTGRVVEFRARQWLRAEPAADTVPLIYPTHFDAGVVRWPKAETKKPNAIV
                     CNSESASLMVRAGVYVLVKRFSAKEERRRLVAAIFDREHVPCEMVGFENHLNYFHERG
                     APLGRELAKGLWAFLNSSALDSYFRQFNGHTQVNATDLRSLHYPSRESLVTLGGQLKG
                     FEFTQGVLDAAVKDILRVG"
     gene            11627..12574
                     /locus_tag="P5316_03535"
     CDS             11627..12574
                     /locus_tag="P5316_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HRR84059.1"
                     /translation="MAGKKPRKVAEAQEILTALGLPSEQRNERAALTLLALVGLPPNK
                     PWRGAANPLCRVTPIMEFVANHYSKKWAPNTRETVRRFTLHQFEQAGLVLSNPDCPDR
                     PTNSPKYCYQVEPRALSLIRKYGTPEWDNALRMYLADAQTLSQRYAQARELKRIPLIL
                     APNTTIRLSPGGQNTLVQKIIDDFCPRFTPAGRPIYIGDTGRKWAYFDEHALQEIGVT
                     VTEHGKMPDVVIHFTEKNWLVLMEAVTSHGPVNPKRLVELTTLFEGSAAGLVFVTAFL
                     DRRGLIKYLREIAWQTEVWVAEDPDHMIHFNGERFLGPY"
     gene            12727..15189
                     /locus_tag="P5316_03540"
     CDS             12727..15189
                     /locus_tag="P5316_03540"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRR84060.1"
                     /translation="MAKKTRSVKWSALWIWGSPGPAHEHRNEYVYLRKTFRLPRSPAA
                     APVRISADNRYVLYVNGQIICRGPARCDPRFQSYDEIDLAPWLRRGKNVIAALAHHYG
                     ESTFQSLERGGWGFLLDGEVRVSRKKAINISSDWTWKGIRAEAYNRKTSRYTVQLGFQ
                     EDFDAAKDVPHWAGRQFDDTGWPEATVRGAATIMPVERFEPRGIPLEQEMPGRFTGIT
                     GRFIGRNGKNWEHCDNIGLLLADERRLPARKTVFADEMTALRPTRSANGMTVRPTRPN
                     EFHAIVLDAGRETCGFLQLNVEAAGGEVIDFHYCEHVRPNGDAIVRSRTGVVNSITDR
                     YRCRKGRQQHQFFSWKGFRYVLVVFRNVRRPLKVHRLDYTFTSYPVERRGAFECSDPL
                     LNRIWETGVWTEQLCMHDAYMDCPWREQAQWWGDARIQWRVNMAVFGDHALFRRGLRQ
                     AAQSQIHNGLTYGLFPSDCHSCILPDYTLVWVCSIWDYYFYTGDDSPIREHFDAVVRA
                     LKWFEKHAGEAHLCGFPGHGMWLFLDWAPLFKAGYNATFSLQYLEALQIAAKMARHLG
                     HDRQARRYRDMAAKVERAVVRVFWDSKGRQFWEGYSQQHRRPYRQVAQHGNSYAILTG
                     VQRRWHRQIGRRVAWILRNHDRLFEANSGGNFQRPGANYPIASSFFYAYVLQALFQTG
                     YGDEALAGIRKLWGRMLDDGATTWCESWNHEPKSFGDTSACHAWSASPTYHLSEQIGG
                     VTAISPGFQTVRIAPQMFDLDWAKVRYPSPRGIIEVRWQRRGRNGMNLHVKLPKGVTG
                     VLDVPGLPRRLLSSGRHEFTSE"
     gene            complement(15290..15363)
                     /locus_tag="P5316_03545"
     tRNA            complement(15290..15363)
                     /locus_tag="P5316_03545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15327..15329),aa:Arg,seq:cct)
     gene            15703..17100
                     /locus_tag="P5316_03550"
     CDS             15703..17100
                     /locus_tag="P5316_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84061.1"
                     /translation="MKTQHAACSILLIVCGFCLSVASATEAEDPRIRPYEKNPFYWQY
                     KGRPVLLLGASDDDNLFQWPLLDKHLDAMKEAGANYIRNTMSDRKDKGFEVYPFKQLP
                     DGKYDLRQWNDEYWTRFANMLRWTHEREIFVQIEIWDRFDYSGDRWPPHPYNPRNNVN
                     YTYEDSGFAPKYPDHPGANKQPFFFTTPAQRNNTVVLEYQHRFVDKMLSLALKYDHVL
                     YCIDNETSGEEKWSAYWADYIHRRAAEANRKVCVTEMWDDWDLKADRHKRTFDHPERY
                     DFADVSQNNQKKGQEHWDNFQWVRRYLSKHPRPLNTVKTYGADGGRYGDTREGLQRWW
                     RHVIGGAASARFHRPDSGLGLSPPSIAALRAARKLESVIKLWDVEPADDLLKDRSENE
                     AYLACAPGRSYAIYFTDGGSVGLDLRDQSGDWKLRWLDLKIGEWAATASQSGSSIATV
                     QAPARGGWIAVITRR"
     gene            complement(17167..17835)
                     /locus_tag="P5316_03555"
     CDS             complement(17167..17835)
                     /locus_tag="P5316_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84062.1"
                     /translation="MPLRCPACGRAVGAEYVDVGLDTATCPHCEESFRPSSALPPVEA
                     SSSRSPTGPSGVLDIEYPPEGGIRIHIPAAGLTAKAMGMFGFALFWNLILAAVFGGFL
                     IGMVKGEIPLAMLLFFLPFIAVGVVMLYIGCRLAWGRTTLWLDRDGFAIVKELFGRQR
                     SRSFLLEDVESFSREVSYTENDKPVYACRARVGRKKVSFGHRLSEADQDWLVAELNRV
                     LSTL"
     gene            complement(17838..18716)
                     /locus_tag="P5316_03560"
     CDS             complement(17838..18716)
                     /locus_tag="P5316_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84063.1"
                     /translation="MSLQCPTCRNVVPAADVNVGADVARCMACGEVFRPSAAMALGPN
                     AVMDEGLPPKPDGPPAGTKVQLEDQGGSLLVRFPPLGFNFGLLFLIVFAIAWWSFLTF
                     FVGFGVWGLFQDDHGQQPAIEAESPLNAEPEAAKKASIENPDSTGGDSELPGWGGILL
                     CLFMLPFFAAGLAMILGILWPLFGRTRVRLAIDGCDYRASLLGIGRTRAAPAADTYVR
                     WTAEPVLSSRRWHSRCGMMDASGGTSEPHILLSLGTWETSVGGHLSLREQEWVFHEMH
                     AWLKRYAEPDRRGRGR"
     gene            complement(18805..20628)
                     /locus_tag="P5316_03565"
     CDS             complement(18805..20628)
                     /locus_tag="P5316_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR84064.1"
                     /translation="MKTAVIAVATALGIVGTVAAREFHVAVNGRDSNSGTQAEPLRTI
                     QRAADLAQPGDVITVYSGVYRERINPPRGGESDANRIVYRAAPGQKVEIKGSEVVKDW
                     LKVQEDTWKVTLPNSFFGEFNPYRDLIRGDWFNPKGREHHTGAVYLNGEWLAEAAKLD
                     DVLKPVENTPLWFARVDDQNTTIWAQFKGVDPNEQLVEINVRRTVFYPDSPGRNYITV
                     RGFTMRHAATPWAPPTAEQIGLIGTHWSKGWIIEDNVVSHSVCSGIALGKHGDEHDNT
                     SANTAEGYVKTIKRGLAAGWNKETIGHHIVRNNTVSHCEQAGIVGSLGPIFCTVTGNV
                     IHDIHVRALFSGAEMAGIKFHGAIDTEISRNHIYRTCRGLWLDWMAQGTRVTANLLHD
                     NQAEDLFVEVDHGPFLVDNNIFLSPTTLLDMSEGGAYAHNLIGGRIVPRPELGRETPF
                     HKPHSTEVAGLRNIVGGDNRFYNNIFAGGNGLADYDAAKLPSWMGGNVFFRGAKPSKY
                     EAHPLVRPEFDPALKLAEKEDGFYLEVGLGELSDSGHPRKLVTTELLGKAAIPDLPYE
                     RPDGTPIRVETDYFGKKRSESNPKPGPFESVDESATSVKVW"
     gene            21304..21588
                     /locus_tag="P5316_03570"
     CDS             21304..21588
                     /locus_tag="P5316_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84065.1"
                     /translation="MNHCPFLAECQYFSDQIDKVQRVGATFKSNQFERIQALAKTYRL
                     EFCASKANFCARHMLAKEVGIEHVPNDLQPSHVLRAKEMIEKLRSTATPP"
     gene            complement(21612..22253)
                     /locus_tag="P5316_03575"
     CDS             complement(21612..22253)
                     /locus_tag="P5316_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRR84066.1"
                     /translation="MNILVSAGPTREYFDSVRFISNPSSGKMGYALAGEAARRGHEVT
                     LVTGPVALEAPRGVKVIRVETAAEMSAACKKAFRKADAAFMTAAVCDYRPRDRVAYKR
                     AKINKPLNVVLEPTEDIAAALGRRKGHRILVAFAMEDHDPYVHAERKLRKKNCDLIVL
                     NGPANVGRNRAEVELFSPQTGWTGPFRGTKAQVARRLIRLVERLQAGHVTDSC"
     gene            complement(22250..22864)
                     /locus_tag="P5316_03580"
     CDS             complement(22250..22864)
                     /locus_tag="P5316_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HRR84067.1"
                     /translation="MTPSPSDQSAPAKDLADYEVLIGVAGGIAAYKVCQVVSRLVQRG
                     CGVTVAMTDAAARFVTPLTFRSLTRRQVFTSMWQAEGYYDPQHLALTERADLFLIAPA
                     TANLIGKFACGIADDLVSTLMVGRDCPAVLAPAMNTRMWENPVVQRNVKTLRELGYQF
                     IDPVEGYLACGALGAGRMAEPEVIVDKVARLLKSNLPRAAGGRQ"
     gene            complement(22845..23093)
                     /locus_tag="P5316_03585"
     CDS             complement(22845..23093)
                     /locus_tag="P5316_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRR84068.1"
                     /translation="MIEALKDDKIINMAGGRFKLTALMQKRWLQLMNGARPMVDPAGK
                     TDLEIITQEILEGKIVPDFAPPAPQAAVEETPSDTVTE"
     gene            complement(23090..23659)
                     /gene="gmk"
                     /locus_tag="P5316_03590"
     CDS             complement(23090..23659)
                     /gene="gmk"
                     /locus_tag="P5316_03590"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRR84069.1"
                     /translation="MAGTLLIISGPSGVGKTTVCKELVKRLDAFLSVSATTRPRRDNE
                     VDGVDYHFLSTEEFENRLQRGEFLEYARVYGGRYYGTPVRPVMDALAAGRVVILEIEI
                     EGTLQVVKKLPQAITIYILAPTPEDQKGRLEGRRKDSAEAMRERLSKADGEIRWAQDC
                     GAYRHFLVNETVEGTVNEIVRIVRESGSK"
     gene            complement(23734..24600)
                     /locus_tag="P5316_03595"
     CDS             complement(23734..24600)
                     /locus_tag="P5316_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRR84070.1"
                     /translation="MTGYGESQCHDDGVYYALELRSVNNRYFKASIKLPETLAVLEPE
                     VEKLLRARLLRGSVACTLRLRNTSAEAAQDVNVAALQRYVEQLSRITTTGPVRIDLAA
                     VLALPGVCQPPEIDESERERQFNILSKLVNQALDRLIEMRRVEGQALREDLLLHCGRI
                     RENLTAVVEQAPQVMKDYHQRLLQRANELLADSSLQLQLDDVRREVAVYAERCDIHEE
                     ISRLRSHLDQFERLCDKGEHAGRKLDFLAQEMLREANTIGSKANDARIAYHVVEIKSA
                     IDRLKEQVQNVE"
     gene            complement(24695..25030)
                     /gene="secG"
                     /locus_tag="P5316_03600"
     CDS             complement(24695..25030)
                     /gene="secG"
                     /locus_tag="P5316_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRR84071.1"
                     /translation="MDILASWVHVVIPIVFLLVCVLLILVILLQKGRGGGLAGAFGGA
                     GGYSAFGAKTGDFFTWLTVGLAGLFVIVACLGVWYYLPDQEKPVPPVQGTNMPGGEST
                     GGGSTTQPS"
     gene            complement(25101..25859)
                     /gene="tpiA"
                     /locus_tag="P5316_03605"
     CDS             complement(25101..25859)
                     /gene="tpiA"
                     /locus_tag="P5316_03605"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRR84072.1"
                     /translation="MRRKFVAGNWKMNLNVAKAKALVEGLKAKIPASVPVDLAVFPPF
                     TMLSAVGEALKGSAIKLGAQNCYFEPEGAFTGEVAPGMLVDVGCELVLIGHSERRHIL
                     GETNAMLKKKIVAALAAGLKVIYCVGEKLDEREAGGTEAVLYQQIHEVLGPDVDLANV
                     VIAYEPVWAIGTGRTATPDQAQEAHAYIRREIGKLYNSQAAQTLRIQYGGSVKASNAK
                     ELMNQPDVDGALVGGASLKVDEFVGIIEGAAAAR"
     gene            complement(25903..26973)
                     /gene="pheA"
                     /locus_tag="P5316_03610"
     CDS             complement(25903..26973)
                     /gene="pheA"
                     /locus_tag="P5316_03610"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRR84073.1"
                     /translation="MTLDDLRKQIDAIDEELVAMLNRRAEVVVQIGRLKSAEGTPVYA
                     PDREKVVFEKIRRANKGPLPDKTLMAIYRELMSGSFALEKPLRIAYLGPQGSFSHLAA
                     VGKFGASVEYESVTDIRGVFEEVSRERADFGVVPIENSVGGGVIDTLDALVETDVQIC
                     AEINRAIHHNLLSLSPLQQLDRVYSKPEVFSQCQRWLSETNLIGKTIAVASTAKAAEM
                     AREEPNAGAIGSALAAQLYGLNIVCENIEDATHNVTRFFVIGRSPAKQTGDDKTSVVF
                     TTKDEPGALVRVLAAFQKEGVNMSFIQSRPSKKRNWEYYFFGDLKGHQNDPPLQAALK
                     EAVHHCLRLNVLGSYPRASEVL"
     gene            complement(27032..28129)
                     /locus_tag="P5316_03615"
     CDS             complement(27032..28129)
                     /locus_tag="P5316_03615"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRR84074.1"
                     /translation="MVLKIAVIGGDGTGPEVVREGLKVLKEVAAKEGFQYSTTEYDFS
                     GKAYLARGGDPNKQNIPIITDEQIAELRQYDGIYLGAVGDPEIAPGILEIGLLLRLRF
                     ELDQYINLRPVKLYPGVETPLKDKGPEHINFDVIRENTEDLYFGGGGFLRKNTPNEVA
                     TQEMIATRFGVERCLRYAFEFCRKKNHKKMLTLVAKRNVLTYAHDLWWRAFNEIGDRD
                     YPDIKRDYNHVDACCMWFVKNPEFYDTIVVPNMFGDIITDLGAMIQGGMGVAAGGNIN
                     PQGVSMFEPIGGSAPKYTGMNVINPIAAIGALAMLLRVVGAQKGDAAVERAGNRVEKA
                     IAATCPKMKSQSAGKMGYGTAQVGDLVCQAL"
     gene            28445..29419
                     /locus_tag="P5316_03620"
     CDS             28445..29419
                     /locus_tag="P5316_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84075.1"
                     /translation="MPFLTPHLRRVGLTGLVGLSLVWGCSHSAGHKPIEFSQNEWVSP
                     SGNVGVQIVTEHYDLRVTSRDVLLQQYLPRFMETAFEAYSSTIQPSHENTDRMVIYLF
                     GTRDEWAAFTRVAYPAQAEVYLHLQAGGYTDQPSATAVTHDLGRDRCLSLLAHEGFHQ
                     YVARHLKRALPAWLNEGLACQWEGFKLVEGRPVFSPKQNFLRRNSLRAALTGKDTFIP
                     LPKLLAMDAGEAVVHTGQVVQSYYAQVWALVLFLQHGANGEHAKAFRELLADAGTDRL
                     DAAVSAYRAATPDAASLSNGEIIFRHYITDDLESCGNDYFKFARKLVF"
     gene            complement(29586..31310)
                     /locus_tag="P5316_03625"
     CDS             complement(29586..31310)
                     /locus_tag="P5316_03625"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84076.1"
                     /translation="MTSERRDVPATGGVCATVSATPGVLFLVRVRTAVLRNTLRQVFQ
                     DRPLKFLGTAASLLLIWAALYELLVQSLLVVNRSVLEGIVATPVMFHFLFLALMGMLA
                     FSNAILCYGILFKRPEIGYLLAAPVEPRDLVIVKFIESLVLSSWSLILLGLPLMMALA
                     RVYSEEWTFYPLFLALFLAFIPLPGALGLLLAWGSVMICPRTPRRALVLVLILIIVVG
                     IWWFRGVLATPMTSADWLERFYDRIGLLGNALFPHTWVSKGIMNALQGRSGLGGYYLY
                     ITMANSLFAGVVAVSVVSRRFMPAYSRAQGGRQRRVGIGGKMLAWLAELMFAYLPKTQ
                     RLLAAKDLKAFFRDPLQWSQMAILLGLLILYVSNAQRTWTDLNDPRLQLLVAFLNSVA
                     ISLILATFTSRFVFPLVSLEGRQFWLLGLLPLRRSRLILAKFLYALTLTLITALIVMG
                     ASLWRLDLSWPLQAAHLVSLAAICIGLCGLSIGMGARMPVLHERNPARIAGGFGGTIN
                     LLMSVVLVVVSLIGVGIMSYREAEAGMGEFVNETLLFWVAATLAFNIVVAAVALVVGI
                     RHFGRLEW"
     gene            complement(31345..32106)
                     /locus_tag="P5316_03630"
     CDS             complement(31345..32106)
                     /locus_tag="P5316_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR84077.1"
                     /translation="MPAAIELREVTKRYGDFTAVDRLSLEIREGELFAFLGPNGAGKT
                     TTIKMIAGLLVADDGDIRVCGYAMGRDGRQAKARMAYVPDQPFLYEKLTGREFLDFVG
                     RMYGLSAATIKARAGGLIERLEMGSFLDQLTESYSHGMKQRTVLAAALLHDPKLLVID
                     EPMVGLDPRTVRTVKEIMWEMTSRGCTVFMSTHTLEVAEAVADRIGIIHRGRLTACGT
                     LEELRAHARRDESLEDIFLRLTADHGNGQAETIPV"
     gene            complement(32109..>32889)
                     /locus_tag="P5316_03635"
     CDS             complement(32109..>32889)
                     /locus_tag="P5316_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84078.1"
                     /translation="IAQDPPRITVEQFKLLDEPLQQYRIVDSRDRRLGTAWSEVRVNR
                     ETASVNGTVLLDGLSNIPALPLKMIPSIRIESQADFDRQGALEGFRLDVHGFLDQKIF
                     VHGERRGIYFPVEMQFGPIRREVPLDYAASRMIGESLNPLACLPTLRLGQSWRMQMLD
                     PLTAVLSGRTEFKSVVARVTGKEAIAHPFEPGRTVDCFIVETVPPSSKAWVDEKGRVF
                     RQEADLPGVGRVTLIMEAYNEEARDNAKSHKSMGRMEPQGN"
BASE COUNT         6461 a        10533 c         9617 g         6278 t
ORIGIN      
        1 tgtaccatgc tcccaagggt ggccagcccg tgtcgttggg gacggcgttc cgcaggtcgt
       61 tccaactgcc cccgcacaga ctggagatcg accagccgcc ggcgctggcg gtgtcggccc
      121 acactccacc gcccggattg ctctgggggg ccttgtaata gcggtggaca agattaccct
      181 cgtgcagccc cgagacgatg atgttcccgg cgttcgaacc gataccgacg gccacgccat
      241 gagctatggt ggccgcgccg cttgggcaag gcagagccac cagaccgtcc gacatcgacc
      301 agatgatcgg gccgttgccg gtgaagctgg acgcaggata gacccacgtg ccataactgc
      361 atccgacggc gtagaccccg tccgcgctga gggcccggat ctcactgtag ggcttggccg
      421 gatcgagcac ccccacagga atgagcgagg cggcttgaag ggttgaagcg acgatcagcg
      481 tcacgaaagc ggcgatgtgt ttcatgttct cctccgggga atgaatgttc gtctcctgga
      541 cgcacgagtc tatcgtgccc tggatcgcac cctctacgtt tgtcgcgaac tcaactgatt
      601 aggagttcgc gcgcgacctc ccacccacta ctgggcgttg cggatcatgg atgctcaacc
      661 catgatccgc acgcccgctc aagagcctga gtgtgtttcc gatacggccc ggcctgcctt
      721 taccgtctgc gtcgcagcag cggcagtgtc agggtcagca aggccagcgt cgccggctcg
      781 ggaatcacag ccacgaagcc tcgtgtgctg ccgtccgccg cccagacgcc atacccggca
      841 agcaccttgc catcatccga tgctgcatac acccgcgtca gcgacgacca catactggta
      901 tcgacgccgg ccgcatccag gagggctttc actgacttcg gcgcaccggt ggtgtcccaa
      961 gtgccgcttg tatcccagac agtcgccacg tagtagccag gaatggtacc cacggcgccg
     1021 aagtaactgt ggcccgccgt cacgccgttg tctgcgaccg tgtaagcgca gctactgttg
     1081 ttgccggtgt cacctccacc tgccggagcc agcgagccga ggaaggtcat cgacgcgtcg
     1141 ccacgtttcc agcggaaggc ctggaaggta gtaccggggc cgttgtaatt ctgaacctgt
     1201 ccgcagatcc attgcacatc gaaatcactc gtcgaggatt tcccgaagcc ggaagaaata
     1261 ccgatgccgt cggctcggga gccggtgctt cctggaaccg tgccgaatga caccgccggt
     1321 ccctcgtaga aagcattgct ggggcttgcg ttatcccttc caacgttcac gccataggca
     1381 ctgacagacc cgacggacct gacagcggtc ccgtcccacc cgctgctggg atcgccgcgg
     1441 tagcgcgcat tccggccggt gtcgttcctc ctgccggcgg tgtaccatcg tccgtcgctg
     1501 ccgggctgga tgcgcaggtc attgctggta cccccgcgca tgttacctgc actaccgagc
     1561 tccccggcgg tggcggtatc gatccaggcg cccccggcca gattggttaa cggggccttg
     1621 tagaagcggt gggtggtatt gttttcgtgc aggcccgaga tgatgatgtt gccggcattc
     1681 gagccaatgc cgacggctac cccatgagcc aggctgttct ggccgctcgg gttgggcagg
     1741 gccacgagcc cgtccgaagc tgaccagacg atcggaacct ggatcccacc aaccgccttg
     1801 ctcgtgccga cggcataact cccgtccgcg ctgaccgcct gcaccagact gtatggggca
     1861 ctgggatcca gcacgccgat ggatgtcagc gacgctccca agaccgccgt tgagcccatg
     1921 gccgtgagta ccacggccac caccaagact ttgacaacct tcattgcctt ttcctcctct
     1981 ccgggcctgg gtgcccggct aagacagaac tgaaaccata gactctttca cagaaaatca
     2041 tcaccctgag gtgtaaaaac acgtgaacaa cgcctctcct ctcgccacac atcgcaatca
     2101 ggagccggtt cgacttatga cttgaccttt tgcccgttcc ctcctttccg atgtcacccg
     2161 acaccagact ccgccgcttc tgatccccac agcgtatgat cgcgcgtcgg ggccgacgga
     2221 tatggcgcga agaccgagca actgggattc caacaccctc agtctaccca gtcgcagtag
     2281 aggtgtcaac tgtttttcgg ttttttcggt cgaccggggt tggccacata ggcaatcggg
     2341 cggtcaggct ccccttgtca ggctcaccgg gaaggcaagg gccctttgtc gcggccacag
     2401 ggaagctctt tgggggtcac ggcgatgagc gcggcccttg aagccggcct attcgtcgcg
     2461 gctttagttt tctgcctcgc ctcaaacctg tccgaggact cctcccgtgc tccagatcac
     2521 gcagtcgcac tttagtgcgg atcaaggctt tgcagccggc ttctcccgga gccgacggtc
     2581 atcaacgtca tacttgtcgc ccctgcgccc ggtccgcctt gggagatggc cattggtacg
     2641 gtcggcgagg aaacggtgcg ctcacaaccg gccgcttttg tgccaggcct ttgaccttac
     2701 gaatgccaaa ctcgccaggc accgatcgga cccgggcgcc gacggttacg gtcaatcgtg
     2761 aaacgctcaa ggttggctct gtgaagcggt ttcgggtttc ccttggcgtg gttggcgtat
     2821 tcggcggttt gcggttcttt tccgttatcg aacgaacata tcaggcgata tgccacgaat
     2881 agaaggtcag gacgcgatga cgatgatgtg tacgaccttg ggaccgtgaa cgccttcgac
     2941 gagaatcatc tcgatgtccg cggtcttgct gggagccgaa atcaacgtca tgttggccgg
     3001 gatgtcatcc ttggagacac gggcggcaaa gtcgagcagg tccggtacga tctgctcggc
     3061 tcggacaagg gcgatgtgat gggggacggc cagactggcc aaccgccgat gagtccctcc
     3121 gctgtcgacg cacagggagc cggtctcggc gatcgcacag ctcacaccag tgatgccaaa
     3181 gtcggcgtcg aaggcggcat cgcggtcatt cacatccatc aggcggacgc cctttccctt
     3241 gagcccgtcg acaatgaggg cgcgggcggg gatgtcctcc tccggcagga tggcgctggc
     3301 cctcccggcg gcggtgacga tttcaaccac cttggcggcg gcggcccgat cgtcgaccac
     3361 ccgctcaggt cgcatgccga cttgttgggc tcgatccatg aacagcgtca cgagatcgac
     3421 gccggacgca gtgacgcggg caacctcaag gtccgaaggc aattcgacgg gggcaccgcg
     3481 atccgtcagc gattcacgaa tgcgagcaaa gaatgcttcc cgggtctggc ctcgcatcat
     3541 cgaccctcct cggcgtcaag ttgtttctgc aagtcggccc agcgggcccg gaacggcttc
     3601 ttggccggca gcgggaaatc gcgatggtcg gtccaacccg agccgtccat cggcagccac
     3661 ttgagccagc cgtccttgga caacaaccac atgaacatcc gcccgaactt caggccgcat
     3721 cggtacaaga acgctgaact cataccgatc cgccagaact tgaacccgat ccgccacgtc
     3781 catggcatcc ggccctgttc gaccagttcg ttgcgcatgt gcagcagata agttggaatg
     3841 tcgatccgga cggggcaggc gtcaaggcat gccccgcaaa ggctcgaggc cgtcggcagg
     3901 tcaggatact cttcgagcga ggccagcagc ggggtaatga gcttgccaat cggtccaggg
     3961 taaacgctct cgtacgcgtg gccgccgatc cgccggtaaa ccggacaggc gttcaggcac
     4021 gctccgcacc ggatgcaacg aagtaccgat cgatactcgc cggccaggat gcgcgaccga
     4081 ccacggtcga gaagcaccag gtgaaactct tcggggccgt cgaagtcccc cggccgtttc
     4141 ggcccggtaa tcaggctggt gtagcaggtc agccgctggc cggtggcgct cttgcccagc
     4201 agcttgagga agatgccgag gtctttcatc cgcggaacga ccttctcgat tcccatcagg
     4261 caaaccacaa cccgcgggcg cgacgagcag aaccgcccgt tgccctcgtt ggtcacaatg
     4321 cagatggtgc ccgtctcggc caccgcgaag ttggccccaa tgatgcccat gtccgcggcc
     4381 ttgaacttgg cgcgcagtac cttgcgggcg gcagccgtca gggcctcggg gtccacggtg
     4441 tattcgatgc ccagcttgtc gcggaataga gcggcgatgt cctccttggt cttgtggatg
     4501 accggtgtga cgatgtggga aggtttctcg ccggctagtt ggagaatgta ttcgcctaga
     4561 tccgtctcga cgacctcgat accggtcttc tcgatctcct ggttcagctc gatttcctcg
     4621 ctgaccatcg atttactctt gacgatcaga ctcgcaccga catcgcgggc gataccggtg
     4681 atgatctcat tggcttgctg gccggtctcg gcgaaatgga cttggccgcc atgcttgcgg
     4741 acgttctcca cgagttgttc aagataacgg tcgaggttct gaagcgtgtg gtccttgatt
     4801 cgtgcggcca gctcgcggat ggccatcggc cggccgagct caggcataat agcccgccgc
     4861 ttgtcgaagt tgagccgcgt ggcccggtca aaggtcgcgc cgatcctcac gtcggccaag
     4921 gccgccgccg cagctttctt gaaatccgga tggaactcat gcgattgcgt tgtttggccg
     4981 ctcatcgagc cgcctccgca gtaacctggc ccgcactcat tcgcgggcga cttgccgtca
     5041 tcgcttcatc cagtatctgc atgatgtgtc tgaactcgac atggagtccg gcacggcggc
     5101 aggctccctg aatgttcatg gtgcaaccgc cgtcattgca gatcaccata tgcgcgccgg
     5161 tcgccttgat acatttgacc ttctcccaga cgatcgcgcc ggacaactcg ctctgcttga
     5221 cagaaaacgt cccgccgaag ccgcagcact ggtcgatttt gtccaggcga cgatactcaa
     5281 gcccgcggaa ttgacggatg agcccttcaa catcctcggg agtcatgtcg atgccgcgat
     5341 tgtggcaact gtaatggtag gtggccacgg ccggataagt caggttccac cgcgaccagt
     5401 cgaccttgag tttcttctgc agaaactcga ctgtctcaaa ggttttcccg gccatcgcca
     5461 ccgcacgggg atacatcacc gggtcatcct tgaacaggtg aggataatac tcgcggacga
     5521 tcgagcagca cgaaccggag ggcgtcacaa cgacctcggc gtcgcggaac acatcgatca
     5581 tccgcccagc gatagcccgg gcctcgtcga aaaaaccgct gttgagcgcc ggctgtccgc
     5641 agcaggtctg gtcttcagga aactccactt ggtgtccgag ccgtcgcagg atgcggacca
     5701 cacactcggc cacgtccgga tagaaagtgt ccgtaagaca ggtgacaaag agggatactt
     5761 tcatggcgtt atcccgaaac tgaagcctcc gtgtcaacga cccacaaact cgccccgcaa
     5821 gaagaagaag atgcagacgc agatcgtcaa caccagcacc gcagccgcaa tgaagaaacc
     5881 tgcccaggaa agcacctgct tttcgttgcg gcgctggccg acgctgttga tgcccaggcc
     5941 gaatccgatc accgccagca gggccgtgac ggcggcggcg gccacgacgg ccatgtaggt
     6001 tcgctgtccg tagaacacag cgagttcctg tactgaaaag cgtcgcacca ccagtgccaa
     6061 caggacaagg aaagtcactg ccgaagccag accagccgct aagctcaccc gcgcctggtc
     6121 ctcatatcga cgaagcgaaa acttggccac gacttgagtc tccagaccgt caggccggca
     6181 agagaagccc acatacactt cgcttacccg gcaaacacgt gttaccacaa gagttgggcc
     6241 ggcacgcggg actcacctgc cgtcccttgc ttgaccgggg cagtttaatg gaaagcggcc
     6301 acggcgtcca ccatcggaac gggtcggcaa ttgcgattca agaacgctcc cttcggcgac
     6361 ggtccctcgc ggaacgccgg ctattgagat gatgaggctg tcgacggcag ttgtcccgta
     6421 atcccgacgc gacagaaggt cttaacggct gttctttctg cggcagtcgc tcgcggcggc
     6481 atggtgggcc ggaccatgtt ctgtctgagc ctgccgcgga tgcggtcagg cgttcgacac
     6541 cggcagacgg acggtgactg tgaatgtctc acccggggat gaccccgctt cgatcgagcc
     6601 gtccaacagt tcgacgagta ccttgcagag tgacagaccc aggccgcagt gcccaccgga
     6661 aaactggcgg gccgcatctc cgcgccaaaa gcggtcgaag actcgcggga catcctgctc
     6721 cgccaccata ctgccgctgt tggtgaccgt gaattcgacg cttccgtcac gacaaatggc
     6781 ctcaacccgg atccacccgc cggtatttgt gtacgtcgcg gcgttgctga ggatgttctg
     6841 aacgatcaga cgcagtttcc cgcagtccga caagatggtg cggggctcat gcagtcgcca
     6901 ttctacctgg agatgccttt gttccgcctg gccggccaac ggcttccagc attctcgaat
     6961 cagtgcggcc agatccaccc cttctcttgc cacctccagt tgattcgcgt cggcacgggc
     7021 gaggtgcagc aggttctcaa ccatccgctg catatgttcg ttgatgccga gacaatcttc
     7081 catggcgttc cggtaggtct cgggctcgcg atctctggaa agggcgacct ccagtatcga
     7141 gcgaacaccc gccagaggcg tgcgaagctc atgggccaca tcggcggtga atcgcctttc
     7201 gcgctgaaaa gcgcgctcca gccggcccag caaaccattc aggcggtcaa tgaccggaag
     7261 caattccgcg ggcacgccgg ctggcgcgat cctgacgccc agctcggatt caccgatgcc
     7321 tgcgatctgg tttgccagcc ggtccagggg tcgcagacct cggcgaacga accaggccag
     7381 cacgccaaga gaaaccgcca ttgcagcagc cccgaccgct gccatcgcga acctgatccg
     7441 agccagggtt gtttcaatgc ccttcgtctg gcggccaaag acgagcgaga ccacaagggg
     7501 ctgttgctga cgccggccgt cgtcctcctg acgcggcgtg aagcggatgc ctgcaatccg
     7561 aacgcgtcgg ccgtccggca accgggtcga ttcgaaggcc ggccggtcga cggtaccctc
     7621 gatctcgatc agctcacgac cttcaaggga ggcggaacgg gcgatggtcc gaccccccgg
     7681 cagccggacc tgatagaact ccgcatgatc tgacggcctg aactcaggga ggttggcctc
     7741 gtcgaaatcc agctcgatct ttccgttttc ttgttccgtc atcgccgcga gcgaacgggc
     7801 tttggcaacc agcgactcgt cgaactcccg ccacaaagcg ttgcggacca gggcataaag
     7861 caggatgccg gacccgagca gcaccaccgt ggcacccacg cctgttccga tcagcaagcg
     7921 agctcgcagt gttcgcacgt cttatcgtcc caacatgtag ccctggcctc ggcgggtgtg
     7981 gatcaggcgg ggcagtcccg gttgttcgat ctttctccgc aagtagccga tgtacacatt
     8041 caccacgttg ctctcgaccg acgcatcgaa ctcatagagg tgttcccaga tatccgaccg
     8101 agagacgatc tgaccggctc gcagggccag aaattcaagc agcgcatatt cccgcgcggt
     8161 caaatcgatt tgccggcctg cccgccgaac agctcgcttg gcggtgtcga tctccaggtc
     8221 ctcgatccgc agcacagggt ctttggcggc gtatttgcgg cggaccagag ctctcacgcg
     8281 ggccagaagc tcttcgaacg caaagggctt gaccaggtaa tcgtcggcgc cgagatccaa
     8341 acctctgacg cggtcctgaa cagtatcctt ggcggtcagc agcagcaccg gagtcgcccg
     8401 gcccgagtgc cgaatccgct tcaggatcgt caacccgtcg acgacgggga gcatgatgtc
     8461 cagaataatg acgtcatagt cgcaggactc cgcataccac aacccctcct cgccgtccgc
     8521 cgagacatcc acggcgaagc cggcctcgcg gagcccttgg ctcaccgatg tccggagcgg
     8581 ttcatagtcc tcgaccaaca ggacgcgcat gataccagaa tggcacggcg ctggcggccc
     8641 cgcaagcacc tgcgccggga atgctccggt catttgtcat catcgtcgtc cgcgtcgtcg
     8701 tcctgatcga cctcccgctt cacaagcgtt ccatctgacg ccactttgat ttcaacctcc
     8761 tggccgttct cgacccattc ggcttcatag accgtttggc cgttcttggt ctcgcgctcg
     8821 atttccttga ccgtggcgcc tttggcttca gccagtatgg tcgctttcac cgctgcagga
     8881 acctggtcga gggaaacttt ctgctcgtcc tcgccgtcgt cgtcatcgtc atcgtcgtca
     8941 ccgtcgtcat ccttcccaaa gatcttgccc gtcgccgaca cgaggatttc ctttcccttg
     9001 ccgttgatct tggcttcaat ctcgtacaaa accatcgtga cttttgcaca ctccagcttg
     9061 gctccggcgg ggaagtgctt ggccacgacg gctttcacat tcgtgggcag ggcatcggcg
     9121 tcaatctcct cttccatttc cacgagatcg ccgccggccg tgaccttggc ctcgacctcc
     9181 ttgccgccgt tgaccttcca ctcggcctcg tacagctcca tcccgttttc cttttcccgc
     9241 tcgaccgcgg tgatcgatgc cccatcggcc agcttacgca gcgtcgcccg ggcctccgcg
     9301 ggaatctgac ccagggtggt cttgggcgcg tctccagccg ttgccacggc aacggcgatc
     9361 gccaagccaa cgaaccccaa cagcgtcaat gctcttttca tggcataact ccttttttga
     9421 aaaaggtttg cgagcgtctc agccttgctc ggctgtcatc tcactatgga cccggaaccg
     9481 caccgagggc ccacgaccaa catagtgtac tcgccgtgaa taagaagatg gtgagaaagc
     9541 cctgaagatc cggtcccgcg cggaatcgga tggtggcact tgccaatggg gcttcggcgg
     9601 tcgccattgt gagggtggcg tcggcagcct cagccgatcg accgcaaccg gtgccctggg
     9661 gctcgacagt ggaacgcgcg ccgtttggcc tcgaagggtc cgcgggcgct ggtctggcac
     9721 agggtcagcg tgtcggcatg gaccaccatc tgccccccgg ccaagggcaa gcggagccgt
     9781 gctatggagt ttatggagcg gtcgaatgat gcggatggga ctatgagccc gggcatacgg
     9841 ctctgaaacc ggtcggccat atgggcggta tcgtcgggct cggaaccctt gcacccggct
     9901 ccgatggtgc caaagccgct acgctgcgcg gcagtgtcgc aagttgtcgc gacgcaaaca
     9961 cgggatataa tgtggattac cggatgaggt cagtcccatt atcgatacca acagtccgtg
    10021 tttggcagga tcgagacgaa accatggctg caaagcctgt cgccgagcta atcgcaacaa
    10081 aaccccaaac gcacccctgt cgggacgaag cgggggttaa ccctttggcg gtcgctgaga
    10141 cggcaaggcg agaggtgaac gcttcgctgg atcgcacccg gcgcagcgcc ttgggccagt
    10201 ttttcactcc gcctgccaca gctcaattca tggcctcgat gtgcagcgtg gtccagccgc
    10261 aagtacacct gctcgatgcc ggggccggcc taggggccct gacggcggcg tgggtgaacc
    10321 agatctgctc tcgatccagc cgccccagca gcgtccgact caccgcttat gaggtcgaca
    10381 cgacgctgct tccggctttg agacaaacta tgtcggcgtg cagacaggct tgcgaggcgg
    10441 ccggaattgc atgcgaatgg gtaattcacg aagccgactt catcgaggcg gctgttgaac
    10501 gcctggcccc tggtcttttt cgcgcggaag accacccgtt caccgtcgcc attctgaatc
    10561 cgccctacaa gaagtttcac gccgattccc ggacccgccg gcttctccga cagatgaata
    10621 tcgaaaccac gaatctctat gctgcttttc ttgcccttgc tctgttatct ctggaagtcg
    10681 gaggagaact cgtcgccatc accccgagaa gtttctgcaa cggaccgtac ttccggcctt
    10741 tccgccagca cctgttccgc catgcgagtc tcacacacgt tcacgtattc gaggcccgtg
    10801 accatgcttt ccgcgacgac gaggtgctcc aggaaaacgt catcatccgt gccgtgaagg
    10861 gggtctcgca gcagcccacg gtatcagtct ctcagagcga aacgcccgat gacttgattg
    10921 acacgcagcg tgaggtgggt tttgaccgcg tcgtacggcc tggggatcac caggcattta
    10981 tccatctcgt gccggacggt cagggcgacc atctggccga cgcaatggta tcgttgccgt
    11041 gcacactcga cgacctcggc ataagcgtaa gcaccgggcg tgtcgtcgag ttccgcgctc
    11101 gccagtggct gcgggctgag ccagcggccg acaccgtccc tttgatttac ccgacacact
    11161 tcgacgccgg tgtcgtccgc tggcccaaag ccgagaccaa gaaacccaac gctatcgtct
    11221 gcaacagcga aagtgcgtcg ctcatggttc gagctggcgt gtatgtcctg gtcaagcgtt
    11281 tctcggccaa agaagagcgc cgacgccttg tcgcggccat cttcgatcga gaacacgtgc
    11341 cgtgcgagat ggtcggattc gagaatcatc ttaattactt ccacgagcgg ggagcgcctc
    11401 tgggacgaga actcgccaaa gggctttggg cgtttctgaa cagctccgcc ctcgactcct
    11461 actttcgcca gttcaacggg catacccaag taaacgccac ggacctccgg tcgcttcact
    11521 accccagtcg ggaatcgctt gtcacgctag gcggccaact gaagggattc gaatttacgc
    11581 agggtgttct tgacgcggcc gtaaaagaca tactgagggt gggatgatgg cggggaagaa
    11641 gccaagaaag gtcgccgagg cacaggaaat cctgaccgct ctcggcttgc catcagagca
    11701 gcgcaacgaa cgggccgctc tgactttact ggcactcgtg ggtcttccac caaacaagcc
    11761 ttggcgcggg gctgcgaatc cgctctgccg cgttaccccg atcatggagt ttgtggccaa
    11821 ccattacagt aagaagtggg cgccgaatac ccgtgagacg gtgcgaaggt tcacactcca
    11881 ccaattcgag caggcaggac tggtgctctc caaccccgac tgccctgatc gaccgacaaa
    11941 cagcccgaag tactgctatc aggtcgaacc tcgggccttg tctctgatcc gtaagtatgg
    12001 aacacccgag tgggacaacg ccctgcgcat gtatctcgcc gatgcccaaa cgctctcgca
    12061 acgatacgcc caggcgagag aactgaaacg tattcctctt attctcgcgc ccaacaccac
    12121 tatcagactg tctccgggtg gccagaacac cctggtgcag aagatcatcg atgatttctg
    12181 cccccggttc acgcccgcag gcaggcctat ctacattggt gataccggca ggaaatgggc
    12241 ctacttcgac gagcacgccc tccaggaaat tggcgtgacg gttacagaac atggcaagat
    12301 gcctgatgtc gtcattcact tcacagaaaa gaactggttg gtcctcatgg aggcggtgac
    12361 cagccacggg ccagtcaacc caaaaaggct ggtagaactc accaccctgt ttgagggcag
    12421 cgcggcaggc ttggtgttcg ttacggcttt ccttgatcgt cgggggctga tcaagtacct
    12481 gcgagagatt gcctggcaaa ccgaggtctg ggtcgctgaa gacccggatc acatgattca
    12541 tttcaacgga gaacggttcc ttggacctta ctgagcacac cgccccggcc aagagcgact
    12601 taccgggccg catcgaaacc aacgagcctg cttcgagcct ccaggcttgc cacgagatcg
    12661 gattgacaca ccccatgaaa tgcctaccat gcgcgccgtt ttccgcgttg tgagaagaca
    12721 acctcgatgg ccaagaaaac gagatcagta aagtggtctg ccctgtggat ctggggcagt
    12781 ccggggccgg ctcacgaaca tcgcaatgaa tacgtctatc ttcgcaagac cttccgattg
    12841 ccgaggtcgc ctgcggcggc gcccgtgcgg atctccgcgg ataaccgata tgtcctgtac
    12901 gtgaacggac agatcatctg tcgcggacca gcccgttgcg acccgcggtt tcaatcgtac
    12961 gacgagatcg acttggctcc ctggctgcgg cggggcaaga acgtgatcgc ggcgttggcc
    13021 caccactatg gtgagagcac gtttcagtcg ctggagcgcg gagggtgggg ctttctgctt
    13081 gacggcgagg ttcgggtaag ccgcaagaag gcgatcaaca tctcctccga ctggacatgg
    13141 aagggcatcc gggcggaagc ctacaaccgc aaaacttccc gctacacggt ccaactgggc
    13201 ttccaggagg atttcgacgc ggcgaaggat gtgccccact gggccgggcg gcagttcgat
    13261 gacaccggct ggccagaggc aacggtgcga ggggcggcca ccatcatgcc cgtggagagg
    13321 ttcgaaccgc ggggcattcc tcttgagcaa gagatgcccg gccgatttac gggaatcact
    13381 ggccgtttca tcggtcggaa cggcaagaac tgggagcact gcgacaacat cggcctcctg
    13441 ctggccgacg aacgccggct gcccgcccgg aagaccgtct ttgcagatga gatgactgcg
    13501 ctgcggccga cccggtccgc aaacggcatg accgtccgac cgacaaggcc aaacgagttt
    13561 cacgctatcg tacttgacgc cggacgcgag acgtgtgggt ttcttcaact caacgtcgag
    13621 gcggccgggg gcgaggtcat cgactttcat tattgcgagc acgtacggcc caacggcgac
    13681 gcgatcgtcc ggtcgcgaac cggcgtggtc aacagcatca ccgaccgcta ccggtgccga
    13741 aaggggcggc agcaacatca gttcttctcg tggaagggct ttcgttacgt tctcgtggtc
    13801 ttccgcaatg ttcgacggcc gctgaaggtt cacagactcg actacacgtt caccagctac
    13861 ccggtcgaga gacgaggggc gttcgagtgc agcgacccgt tgctcaatcg catctgggag
    13921 accggcgtat ggaccgaaca gctctgcatg cacgacgcgt acatggattg cccgtggcgc
    13981 gaacaggcgc agtggtgggg cgacgcgcgc atccagtggc gggtgaacat ggcggttttc
    14041 ggtgaccatg ctcttttccg gagaggtctt cgccaagcgg cccagtccca gattcacaac
    14101 gggctgacct acggtttgtt cccctccgac tgccactcct gcattctgcc ggactacacc
    14161 ctagtctggg tctgctccat atgggactat tacttttaca ccggcgacga ctcgcccata
    14221 cgcgagcact tcgacgcggt ggtcagagca ttgaagtggt ttgagaaaca tgcgggcgag
    14281 gcgcacctgt gcggattccc gggtcacggg atgtggctgt tcctggattg ggcaccgttg
    14341 ttcaaggcgg gctacaatgc gaccttcagc ctccagtatc tggaggcctt gcagattgct
    14401 gcgaagatgg cccggcacct gggacacgac cggcaggcgc gtcgataccg cgatatggcg
    14461 gccaaggtcg aacgggcggt ggtgcgcgtt ttctgggatt ccaagggcag acagttctgg
    14521 gaaggctaca gccaacaaca ccgccggccg tatcgccagg ttgctcaaca cggcaacagc
    14581 tacgcgatcc ttaccggcgt gcaacgacga tggcatcgac agatcggacg tcgagtggca
    14641 tggattctca ggaatcatga tcgcctgttc gaggccaaca gcggcggcaa cttccagcgg
    14701 ccgggggcca actacccgat tgcctcgtcc tttttctacg cttacgtcct gcaagcgttg
    14761 tttcagaccg gctacggcga tgaggctctt gcgggcatcc ggaaactctg gggccggatg
    14821 ctcgatgacg gtgcgactac ctggtgcgag tcgtggaacc acgagccgaa gtcatttggc
    14881 gataccagcg cgtgccacgc ctggtccgcc agcccgactt atcacctttc cgagcaaatc
    14941 ggcggtgtga ccgcaatcag ccccggtttc cagaccgtgc ggatcgctcc gcagatgttc
    15001 gatctcgatt gggccaaggt gcgttacccc tcgccgcgag gaattatcga ggttcgatgg
    15061 cagcggcgag gtcgcaacgg catgaatctc cacgtcaagc tgccgaaagg cgtcacgggt
    15121 gtgctggacg tgccagggct gcccaggaga ctgctctcga gcggtcgcca cgagttcacg
    15181 tcggaatagc cgtgcgggtc gatttgcaga tcaatcaacc tcatctgcgt cgaccactgc
    15241 ccgcatactc ttcatcgcca cgtcgtacct ggtacggtgt tcagcaaacc gcgcccgaca
    15301 ggattcgaac ctgtgacccc cggtttagga aaccggtgct ctatcctact gagctacggg
    15361 cgcaaaccgc tctgagcctg ccccgagatg gctcataggc attataaccc ggttgcagcc
    15421 ggtctgcacg cttgcgtcag cggtccccga gcccccttac tccattcggg tgaaagtggc
    15481 gttcccgcag cccccctccg agggtccgac agaccacgac tgccgccggc acgatttgct
    15541 ccacccatgt cgcgagggta caagtaagca tcagccatac tgccgccatt cccgcgtcac
    15601 aggggtattg ccggacaagg gcttgaacct tttacaggta ttaaagcctt ttcggctcct
    15661 caggcaccgg aacggcctga gacaccgcat gagaggtctc tgatgaagac acaacatgct
    15721 gcttgctcga tcctcctcat cgtctgcggg ttctgcctct ccgtggcttc ggccaccgag
    15781 gccgaggacc cgcgaatccg tccttacgag aagaacccct tctactggca gtacaagggc
    15841 cggcccgtcc tgttgctcgg ggccagcgac gacgacaacc tgttccagtg gcccctactc
    15901 gacaagcacc tcgatgcgat gaaggaggcc ggcgccaact acatccgcaa caccatgagc
    15961 gaccggaagg acaagggttt cgaggtgtac cccttcaagc agttgcccga cggcaaatac
    16021 gacctccgcc agtggaacga cgaatactgg acacgattcg ccaacatgct tcgctggacg
    16081 cacgagcggg agatcttcgt gcagatcgag atatgggacc gattcgacta cagcggcgac
    16141 agatggccac cgcacccgta caacccgcgc aacaacgtca attacacgta cgaggattcc
    16201 ggtttcgccc ccaagtaccc cgaccacccc ggggccaaca agcaaccctt cttcttcacc
    16261 acgcctgctc agcggaataa caccgtcgta ctcgaatacc agcaccggtt tgtggacaag
    16321 atgttgtcgc tggcgctgaa atacgatcac gtgctctact gcatcgacaa cgagacctcc
    16381 ggcgaggaga aatggtcggc ttactgggcc gactacatcc accgtcgcgc ggccgaggca
    16441 aacaggaaag tctgtgtcac cgagatgtgg gatgactggg acctcaaggc cgatcgccac
    16501 aagcggacct tcgatcaccc cgagcgatac gatttcgcgg acgtctcgca gaacaaccag
    16561 aaaaagggcc aggagcactg ggacaacttc cagtgggtgc gccgctactt gagcaagcac
    16621 ccacggccgc tgaacaccgt caagacctac ggggccgacg gcgggcgata cggtgacacg
    16681 cgggagggtc ttcagcgatg gtggcggcat gtgatcggag gggcggcctc ggcccgcttc
    16741 caccggccgg actcgggcct gggcctctcg ccgccgtcca tcgccgcgct gcgggccgcg
    16801 cggaagctgg aatcggtcat caagctctgg gatgtcgagc ccgccgacga tcttctgaaa
    16861 gatcgatctg aaaacgaagc atacctggcg tgtgcgccgg gccggtcgta cgccatctac
    16921 ttcaccgacg gcgggtccgt ggggctcgac cttcgtgatc agtctggcga ctggaagctc
    16981 cgctggctcg atctcaagat cggcgagtgg gccgcgacag cctcccagtc cggcagctcg
    17041 atcgcaactg tccaggctcc cgcccgcggc ggctggatcg ccgtcatcac aaggaggtga
    17101 tcccctgggc gacgtgatta agcctctgcg agctcatcgt cgcagcaatc aacgagtaat
    17161 ctgcgttcac agggttgaaa gaaccctgtt cagctccgcg accagccaat cctgatcggc
    17221 ttccgacaga cgatgaccga agctgacttt cttacgcccg acccgcgcac ggcacgcgta
    17281 caccggcttg tcgttttccg tgtaactgac ctcgcgagag aagctctcca cgtcctccag
    17341 tagaaacgag cggcttcgct gacgcccgaa taactccttc acgatggcaa agccgtcgcg
    17401 atccagccac agtgtcgtcc gtccccaggc cagccggcac ccgatgtaca acatcaccac
    17461 gccgacggca atgaacggta gaaagaacag cagcatcgcc aacgggattt cgcccttcac
    17521 catgccgatc aggaatccgc cgaacacggc agccaggatc aggttccaga aaagggcgaa
    17581 gccgaacatc cccatcgcct tggcggtcag tccggccgcc ggaatgtgaa tgcgaatccc
    17641 gccctccggc gggtactcga tgtcgagaac gcccgacggc ccagtgggcg atctgctcga
    17701 agacgcctcg acaggcggca atgcgctcga aggccggaat gactcctcac agtggggaca
    17761 ggtggcggtg tccagcccca catcaacgta ttcggcaccg acagcccggc cacatgccgg
    17821 gcatcgcagc ggcatgtcta cctcccgcga cccctccggt ccggttccgc atatcgcttc
    17881 agccacgcgt gcatctcgtg aaatacccac tcctgttccc gcaggctgag gtgaccgccg
    17941 acggatgtct cccaggttcc cagcgacagc agaatgtgcg gctcggaggt tccgccggac
    18001 gcatccatca ttccacacct gctgtgccac cgacggctgc tcaggacagg ttcggccgtc
    18061 caccgaacgt acgtatcggc cgctggagcg gcgcgggtac ggccgatgcc cagaaggctg
    18121 gctcgataat cgcagccgtc gatcgccagt cgcactcgtg ttcgcccaaa caacggccac
    18181 aggattccca ggatcatggc aagccccgcc gcgaagaatg gcagcatgaa caggcacagg
    18241 aggatgccgc cccagcctgg gagctcggag tcgccgcccg tcgagtcggg attctcaatg
    18301 gaggccttct tggcagcttc gggctcagcg ttcaaggggc tttccgcttc gatagccggc
    18361 tgttgaccgt gatcgtcttg gaaaagcccc cacacgccga agccaacgaa gaacgtcaga
    18421 aaactccacc acgcaattgc aaaaacaatc aggaacagaa gcccgaaatt gaatcccagc
    18481 ggcggaaacc gcaccagcag cgaaccgccc tggtcttcaa gttgaacctt cgtccccgcc
    18541 ggaggcccgt cgggcttcgg tggaaggccc tcgtccatga cggcgttggg gcctaatgcc
    18601 atcgccgcgc tcggccgaaa cacttctcca cacgccatgc accgcgccac gtccgcccca
    18661 acattgacgt ccgctgccgg gacgacgttc cggcacgtcg ggcattgtag actcataagt
    18721 atttcttcct gctcactctt acttttaccg gtctgcctct caaatccgac tgcgccggcc
    18781 ctgcttgacg agagtgcacc tgaatcacca gaccttgacg ctcgttgccg actcatcaac
    18841 gctctcaaac ggcccaggct tagggttcga ttcgctccgc ttcttgccga agtaatcggt
    18901 ctcgacgcga atcggcgtgc catccggccg ttcgtacggc aaatccggaa tggccgcctt
    18961 gccgagcagt tcggtggtca ccaacttgcg tgggtgcccg gaatccgaaa gctccccgag
    19021 cccgacttca aggtaaaaac cgtcctcttt ttccgccagc ttgagggcgg ggtcgaattc
    19081 cggcctgacc agcggatgcg cctcatactt cgatggcttg gcccctctga aaaacacgtt
    19141 cccgcccatc catgagggca gcttcgccgc atcgtaatct gccagaccgt tgccgcccgc
    19201 aaaaatgttg ttgtagaagc gattatcgcc tccgacgatg ttccgaagtc ctgcgacctc
    19261 ggtcgagtgc ggcttatgga acggggtctc gcgtcccaac tcgggtcggg gcacaatgcg
    19321 cccgccgatc agattgtgtg cgtaagcccc gccttcggac atgtccagca gcgtcgtcgg
    19381 ggagagaaag atgttgttgt ccaccagaaa cggcccgtgg tccacctcga caaacagatc
    19441 ctcagcctga ttgtcgtgca acagattcgc agtgacgcgc gtaccctggg ccatccaatc
    19501 aagccacaga ccgcgacacg tccggtagat gtgattacgg ctgatctccg tgtcgatcgc
    19561 cccgtggaac ttgattcccg ccatttcggc gcccgaaaac aatgcccgca cgtggatgtc
    19621 atgaatgacg ttacccgtca cggtgcagaa gatcggaccg aggctgccga cgatgcccgc
    19681 ctgttcgcag tgcgagacgg tgttgttgcg aacgatgtgg tggccgatgg tctctttgtt
    19741 ccagccggcg gccagtccgc gtttgatggt cttgacgtac ccctccgcag tgttggccga
    19801 cgtgttgtca tgttcgtcgc cgtgttttcc cagggcgatg cctgagcaga ccgagtggct
    19861 gaccacgttg tcctcgatga tccatccctt gctccaatgg gtgccgatca gaccgatctg
    19921 ctcggccgta ggcggagccc acggggtcgc cgcgtgccgc atggtaaagc cacgcacggt
    19981 gatgtagttc ctgcccggtg agtccggata gaacaccgtt cgccggacgt tgatttcgac
    20041 cagttgctcg ttgggatcga cccctttgaa ctgggcccag atggtggtgt tctgatcgtc
    20101 cacccgcgcg aaccacagcg gcgtgttctc cacaggcttc aggacatcat ccagcttggc
    20161 cgcctcggcc agccactcgc cgttgagata aaccgccccg gtgtgatgct cgcggccttt
    20221 ggggttgaac cagtctccgc ggatcaggtc gcggtaggga ttgaactcgc cgaaaaatga
    20281 attcggcagc gttaccttcc aggtgtcttc ttggaccttc agccagtcct tgacgacctc
    20341 cgagcccttg atctcgactt tctgccccgg cgccgcccga tagacgatgc ggttggcgtc
    20401 cgactcaccc ccgcgcggcg ggttgatgcg ttcgcggtaa acgccggaat acacggtaat
    20461 cacatcgccg ggttgtgcca gatcggccgc ccgctgaatc gtccgcagcg gttcggcctg
    20521 cgtgccgctg ttggagtctc ttccgttgac cgcaacgtga aactccctgg cggcaaccgt
    20581 gccgacgatt ccaagggctg tcgcgacagc gataacggct gttttcatcc ttctgctcct
    20641 gtttccagtg gccggcctgc gaccggcccg tcattgccgc ccagcctcgt gccgacgaag
    20701 gtctctcccc tgtcaggctg gatcgtcgca aaacacgata cccctgcccg ctccctgact
    20761 caacgccggc agaaggtact gaatggtcgc ccagggcaat cgcatccaaa tcccctggca
    20821 caggacccgg tttagtctct tccgtcgcga ctggtgcgcg ttttgcgcgc atgtttctgg
    20881 accgcggtac gcttgggttg cggcccgcaa aaaggcggtc cgccttaggc aatcctcccg
    20941 caggccgctc catccgggga tcctccaact catcaaaaaa gcgcagcagg ccacacgcag
    21001 ctccaacgcc catgttgcag ctcccgaaaa aggaagaccc tctaaaaaac gcgaggcctc
    21061 gtaaccccta tgcccgaagt catccgcaat tcaaccagag cgtaatcctt acagatgcct
    21121 aacacacaga aaagacgaga cgaagctcat cgtttagatg cgattgccct gcgatcccgt
    21181 ccctgcccgt tcggctgggc ttgcagaaac accgaatatg ggacgctgcg catattgtat
    21241 tcttgattct tgagaaacag gacaatcagg gcaaacggcg cattctgcgc aagggatccg
    21301 cggatgaacc actgtccgtt cctggccgag tgtcagtact tcagtgacca gatcgataag
    21361 gtccagcggg tcggcgcgac cttcaagagc aaccagttcg agaggatcca ggcgctggcc
    21421 aagacctacc gtcttgagtt ctgcgcctca aaagccaact tctgtgcgcg ccacatgctc
    21481 gccaaggaag ttgggatcga gcacgtgccg aacgatcttc agccgagtca tgtcctccga
    21541 gccaaggaga tgatcgaaaa gctccgctcg acggccacgc ctccatagca cgcttatcgc
    21601 ggatcgtcgt ctcagcagga atcggtgaca tggccggcct gcaacctctc gaccaaacgg
    21661 atcagccgac gagctacctg ggctttcgtc ccgcgaaacg ggccggtcca cccggtttgg
    21721 ggcgagaaga gctcgacttc ggcgcggttg cggcccacgt tcgccggtcc gttcagcaca
    21781 atcaagtcgc agttcttctt gcgcagtttg cgttcggcat ggacgtaggg atcgtgatct
    21841 tccatcgcaa aagcgacaag aatccgatgc cctttgcgtc ggcccagggc ggcagcaatg
    21901 tcctcggtgg gttcgagcac gacgttcaag ggcttgttta ttttggctcg tttgtaggcg
    21961 acgcggtccc gaggtcggta atcgcagacc gccgccgtca tgaacgccgc gtcggccttg
    22021 cgaaaagcct tcttgcaggc cgcggacatt tcggcggccg tttcaacgcg gatcactttg
    22081 acgcctcgtg gagcttcgag ggcgaccggt ccggtcacca gcgtcacctc gtggccgcgg
    22141 cgggcggcct cgccggccaa ggcgtacccc atctttcctg acgaggggtt ggagatgaaa
    22201 cggaccgagt caaagtattc gcgagtggga ccggcggaaa caaggatgtt cactgccgac
    22261 ctccagcggc cctgggtagg ttgcttttga gaaggcgggc caccttgtcg acgatgacct
    22321 cgggctcggc catacggccc gcgccgagcg ccccgcatgc caggtagccc tcgacgggat
    22381 cgatgaactg ataacccagc tcacgcaagg tcttcacgtt gcgctggacg acggggtttt
    22441 cccacatccg ggtgttcatc gcgggagcaa gaacggcggg gcaatcccgc cccaccatca
    22501 gcgtgctgac caggtcgtcg gcgatgccac aggcgaactt gccgatcaag ttggcggtcg
    22561 caggggcgat caggaacagg tcggcccgct cggtcagagc caggtgctgg ggatcgtaat
    22621 acccctcggc ctgccacatt gaggtaaaaa cctgccgacg ggtcagcgac cggaatgtga
    22681 gcggggtgac gaatcgcgcg gccgcatcgg tcatcgccac ggtcacgccg cagcctcgct
    22741 gtaccagccg cgaaaccacc tggcagacct tgtaggcggc gatgccccca gcgacgccaa
    22801 tcagcacttc atagtcggca agatcctttg ccggggcgga ctggtcactc ggtgacggtg
    22861 tcactgggcg tctcctccac agccgcctga ggggccggtg gagcgaagtc gggaacgatc
    22921 ttgccctcaa gaatctcctg cgtgatgatc tcgaggtccg ttttgcccgc aggatcaacc
    22981 atggggcggg cgccgttcat caactggagc cagcgcttct gcatcagggc ggtcagcttg
    23041 aagcggccgc ccgccatgtt gatgatcttg tcgtccttca gtgcctcgat catttggaac
    23101 cactctcccg tactatacgg acaatctcat tgaccgtccc ctcgacggtc tcgttcacca
    23161 gaaaatggcg ataggccccg caatcctggg cccagcggat ctctccgtcg gccttgctca
    23221 gccgttcgcg catggcctcg gcggagtcct ttcgccgccc ttcaagccgc cccttctggt
    23281 cctcaggggt cggcgccagg atgtagatgg tgatcgcctg cggcaacttc ttcaccacct
    23341 gcaacgtccc ctcaatttcg atctccagga tgaccacccg gccggcggcc aaagcatcca
    23401 ttaccggcct gaccggcgtg ccgtaatatc gccccccgta caccctggcg tactccagga
    23461 attcgcctcg ttgcagccgg ttctcgaact cctcggtcga caggaaatgg tagtccaccc
    23521 cgtcgacctc gttgtcccgc cgcggccgcg tcgtcgccga aacgctcaga aacgcatcga
    23581 ggcgtttgac caattccttg caaacagtcg tcttgcctac gccgctgggt ccgctgatga
    23641 tcaacagggt tccggccatg tgtccgcctg tcgagccaca catccggcgg cgggggcacg
    23701 cccatgagtc gccccgctcg ccgctaccac ccgctattcc acgttctgca cctgctcctt
    23761 gaggcgatcg atggcgcttt tgatctccac cacatggtag gcgatccgtg cgtcattggc
    23821 cttgctgccg atggtgtttg cctcccgaag catttcctga gcgaggaaat cgagcttgcg
    23881 tcccgcgtgc tcgcctttgt cgcacagtct ctcgaactgg tcgaggtgcg agcgaagccg
    23941 cgagatctcc tcgtgaatat cgcaacgttc cgcgtagacg gctacctcgc ggcggacgtc
    24001 gtcgagctga agctgcaacg aggagtcggc cagcaattca ttcgccctct gaagcagccg
    24061 ctgatggtag tccttcatca cctgaggggc ctgttcgacg acggcggtga ggttctcccg
    24121 aatgcgcccg caatgaagca acagatcctc ccgcagggcc tgaccttcca cccgacgcat
    24181 ttcgatgagt cggtcgagtg cctggttgac cagcttgctc agaatgttga attgtcgttc
    24241 ccgctcgctt tcgtcgatct ccggcggctg gcaaacgcca ggcaaagcca gtacggccgc
    24301 aagatcgatc cgtaccggcc ccgttgtcgt gatgcggctc aattgctcga cgtaccgctg
    24361 gagggccgcg acgttgacgt cttgagcggc ctcagcactg gtgtttctca accgcagggt
    24421 gcaggccacg ctgccgcgaa gcagacgggc gcgcaacagt ttctcgacct cgggctcaag
    24481 gacggccagc gtctcgggca gtttgatcga cgccttgaag tagcggttgt tgacgcttcg
    24541 cagctcgagc gcatagtaaa cgccgtcgtc atgacactgg gattcaccgt atccagtcat
    24601 cgagaaaatc atgtcgtcga cgttcttcct tgtcgcctgg tctgcaaagc cgtctctgcc
    24661 ggccccatgg cccggtatcg cggctgacgc ccggtcagga gggctgggtg gtgctcccgc
    24721 cgccggttga ttccccgccg ggcatgttcg tcccctggac ggggggcacg ggcttctcct
    24781 gatcgggaag gtaataccac acccccaggc aggccacgat cacgaacagt ccggccaggc
    24841 caacggtcag ccaggtaaag aaatcgccgg tcttggcacc gaaagcgctg tacccgccgg
    24901 ccccgccgaa cgcgcccgcc agaccgccgc cgcggccttt ctggaggagg atcaccagaa
    24961 tcagcagaac gcacacaagc agaaacacga tggggatcac cacatgaacc cacgatgcga
    25021 gaatgtccat gagaactgtt cctttccttt gcgacgatcc cttgtgtcgt ccggggcgtc
    25081 tcgggcacat ttcccggccc ttatcgggcg gcggccgcac cctcgatgat tccgacgaac
    25141 tcatcgacct tgaggctcgc cccgccgaca agagccccgt cgacgtcggg ctgattcatc
    25201 agctccttgg cgttcgaggc cttcacgctg cccccgtact gaatccgcag ggtctgggca
    25261 gcttggctat tatacagctt gccgatttcc cggcgaatgt aggcatgggc ttcctgggcc
    25321 tgatcgggcg tggccgtcct gccggttccg atcgcccaga cgggctcgta ggcgatcacc
    25381 acgttggcca agtcgacgtc gggtcccaaa acctcatgaa tctgctggta caggacggcc
    25441 tcggttccgc cggcctcccg ctcatccagc ttctcaccaa cgcaatagat aaccttcagg
    25501 ccagccgcca aggcggcaac gattttcttc ttgagcatgg cgtttgtctc gcccagaatg
    25561 tgccgtcgct cgctgtgccc gatgagcacc aactcgcacc cgacgtcgac gagcatgccg
    25621 ggcgcgacct cgccggtgaa ggctccctcg ggttcaaagt agcagttctg ggctcccaac
    25681 ttgattgcgg agcccttgag cgcctcgccg acggcggaca acatggtaaa gggcggaaac
    25741 accgccagat cgaccgggac tgaggcaggg atcttcgcct tgagcccctc caccagggcc
    25801 ttggccttgg ccacgttcaa attcatcttc cagttgccgg ccacaaactt acgtcgcatg
    25861 ctcttgttct ccaagttcag tcgaaagacc gcttcgcccg gctcacaata cttccgaggc
    25921 tcgcggatat gaacccagca cgttcagtct caggcaatga tgaacggctt ccttcagggc
    25981 cgcctgtaac ggcgggtcgt tctgatgacc cttcagatcg ccgaagaagt agtactccca
    26041 gttgcgcttc ttgcttgggc gggattgaat gaaggacatg ttgacccctt ccttctggaa
    26101 agccgccaac accctcacca gtgcgcccgg ctcatccttg gtggtgaaca cgaccgacgt
    26161 cttgtcatcg ccggtctgct tggcgggact gcgaccgatc acgaaaaacc gcgtcacatt
    26221 atgggttgcg tcctcgatgt tctcgcaaac gatgttcagt ccgtacaact gggcggccag
    26281 cgcgctgccg atcgcaccgg cgttgggctc ctcgcgggcc atttcggcgg ccttggcggt
    26341 gctggcgacc gcaatggtct tgccgatgag gttcgtctcg ctcagccatc gctggcactg
    26401 cgagaacacc tcgggtttgg aataaacgcg atcgagctgc tgcagcgggc tgagggagag
    26461 cagattgtga tgaatcgccc ggttgatttc cgcgcagatc tggacgtcgg tttcgaccag
    26521 ggcgtcgagg gtgtcgatca cgccgccgcc gacggaattc tcgatcggca cgacgccgaa
    26581 atccgcccgc tcccgagaaa cctcctcgaa aacaccccgg atgtcggtga ccgattcata
    26641 ctcgacggag gccccgaact tgcccaccgc ggccaggtgc gagaagctgc cctgcgggcc
    26701 gagataggcg atccgcagcg gcttctcgag cgcgaatgag ccgctcatca gctcacggta
    26761 gatggccatc agcgtcttgt cgggcagggg tcctttgttg gcccgacgga ttttttcgaa
    26821 gaccaccttt tcgcggtccg gcgcatagac gggcgtacct tcggcgcttt tgagcctgcc
    26881 gatctgaacg acgacctcgg cgcggcggtt cagcatcgcg acgagctcct cgtcgatggc
    26941 atcaatctgc ttgcgaaggt cgtcgagagt catgttcgca cccgcggccg gtgatcagca
    27001 aacggccaag agctgcaagc cgacggcttc atcataacgc ctggcacacc aagtcaccca
    27061 cttgcgcggt gccgtatccc atcttgccgg ccgactgcga tttcatcttc gggcaggtgg
    27121 cggcaatggc cttttcgacc cggttgccgg cccgttcgac ggccgcgtca cccttctgcg
    27181 cacccaccac cctgagcagc atcgccaggg ctccgatggc cgcgatcgga ttgatgacgt
    27241 tcatgccggt gtacttgggc gcgctgccgc cgatcggctc aaacatgctg acaccctgcg
    27301 ggttgatgtt gccgccggcg gccacaccca tgccgccctg gatcatcgcc cccaggtcgg
    27361 tgatgatgtc gccgaacatg ttcggcacca cgatggtatc gtagaattcg gggtttttca
    27421 caaaccacat gcagcatgca tccacgtggt tgtagtccct cttaatatcg ggatagtccc
    27481 ggtcaccgat ctcgttgaac gcccgccacc acagatcatg ggcataggtc aacacgttcc
    27541 gtttggcgac cagggtcagc atcttcttgt ggttcttctt gcggcagaac tcaaaggcat
    27601 atcgcaagca acgctcgacg ccgaaccgag tggcgatcat ttcctgcgta gccacctcgt
    27661 tgggcgtgtt cttccggagg aagccgcctc cgccaaagta cagatcctcg gtgttctctc
    27721 ggatgacatc gaagttgatg tgctcggggc ccttgtcctt gagcggggtc tcgacgccgg
    27781 gatacagctt gaccggtcga aggttgatgt actggtccaa ctcgaatcgc aatcgcagca
    27841 gcaggccgat ttcaaggatc ccgggcgcga tctcggggtc gccgacggcc ccgaggtaga
    27901 tgccatcgta ttgccggagt tcggcgatct gctcgtcggt gatgatgggg atgttctgct
    27961 tgttcggatc gccaccgcga gccagatagg ccttgccgct gaaatcatac tccgtggtcg
    28021 agtactgaaa accctccttg gccgcgacct ccttgagaac cttcagaccc tctctgacga
    28081 cctccggccc cgtgccgtcg ccgccgataa ccgcgatctt taacaccaca cctgctcctt
    28141 ggaggaccag acaacagggc cggacacccg accctggaaa aggcgagcca gataaggtat
    28201 cacatgcatc tggttgcttc aagcgtctct ggaagctagg ccgacgtcgc cttcaggaat
    28261 gacctatccc ttggcggcga aaggctttag aaccttacgg ccggatatct gtgcggcttg
    28321 cgcgcatgtt tccggatcgc ggtacgcttg ggttgcggcc cgcaaaaagc cggtctgcct
    28381 cagcacacac atgatccttg ctgccgctgc cacgggccgg atggctgaca atggaggatt
    28441 ctcgatgccg tttctcacac cacacctgcg cagggtagga ctgacggggc tggtgggact
    28501 gtccctggtc tggggttgct cccattcggc agggcacaag cccatcgagt tctctcagaa
    28561 cgaatgggtt tctccgagcg gcaacgtcgg cgtgcagatc gtgacggaac actatgacct
    28621 gcgggtcacc agccgggacg tgctcttgca gcagtacctg ccccggttca tggaaaccgc
    28681 gtttgaagcc tacagctcga cgatccaacc gagccatgag aacaccgacc ggatggtcat
    28741 ctatctattc ggcacgcgag atgagtgggc ggcctttacc cgcgttgcct atcccgcgca
    28801 ggccgaggtc tatctccatc tccaggccgg cggatacacc gaccagccga gcgccacagc
    28861 cgtaacgcat gacctcggac gcgatcgctg cctgtcgttg ctggcccacg agggctttca
    28921 ccaatacgtc gcccgacacc tgaaaagagc cctgccggca tggctcaatg agggcctcgc
    28981 ctgccagtgg gagggcttca agctggtgga aggcagacct gtcttcagcc cgaagcagaa
    29041 cttcctccgg cgtaacagtc tgcgagccgc attgaccggg aaagacacat tcattccact
    29101 gcccaagttg ctcgccatgg atgcaggaga agccgttgtt cacaccgggc aagtggtaca
    29161 atcctactat gcgcaggtct gggcactcgt tctctttctt caacacggag ccaacggcga
    29221 gcacgccaag gcttttcgag aactgctggc cgacgccggt acagaccgac tcgatgcggc
    29281 ggtcagcgcc taccgagccg caacgccgga cgcggcctcg ctcagcaacg gcgagatcat
    29341 cttccgccac tacatcacgg atgacctgga aagctgcggg aacgactatt tcaagtttgc
    29401 ccggaaactg gtcttttgag ctcctgaagg aatctgagag cgtcagaacg cgacgtcagt
    29461 tagaggggcc acagtgtgag acgggcttcc ggccgtcatg aaacggcgag acgccaaaac
    29521 ggcaggatgc cgatgcgaca ccgggtagga accaggggat cgatcgcgtg ctcgcaaatg
    29581 tgctgctacc actcgagtcg cccgaaatgg cggatgccca ccaccagcgc cacggcggcc
    29641 acgacgatgt tgaaagccag cgtcgccgca acccagaaca gcagcgtctc gttgacgaac
    29701 tcgcccatgc cggcctcggc ctcgcggtag ctcatgatcc caacgccgat cagcgacacc
    29761 accaccaaca ccacagacat caacagatta atggtgccgc caaacccccc ggcgatcctg
    29821 gcggggttgc gctcgtggag aaccggcatg cgggccccca tcccgatgct gagcccgcag
    29881 agcccgatgc aaatcgccgc gagggagacc aggtgagctg cctgcaacgg ccatgacaga
    29941 tcgagccgcc ataacgaagc gcccatgacg atcagcgccg tgatcagcgt cagcgtcaga
    30001 gcatagagga atttggcgag aatgaggcgg cttcgtctca gcggcagcag acctagcagc
    30061 cagaactgcc ggccctccag ggacaccagc gggaagacga aacggctggt gaacgtggcc
    30121 agaatcaggc tgatggccac cgaattcaag aacgcgacca gcagttggag tcgcgggtcg
    30181 ttcagatcgg tccaggtccg ttgcgcgttg ctcacgtaca ggatcaacag acccaacaga
    30241 atcgccatct gcgaccactg caacggatcg cgaaagaaag ctttcaggtc cttggccgcc
    30301 agcagccgct gggtcttcgg caggtacgcg aacatcaact ccgccaacca ggcaagcatc
    30361 tttccgccga tgcccactct tcgctgtcga ccgccttgag cccgactgta cgccggcatg
    30421 aaccgccgcg agaccaccga caccgcgacg acgccggcaa acaacgaatt cgccatggtg
    30481 atgtagaggt aatagcctcc aaggcccgat ctgccctgga gggcattcat gattcccttg
    30541 gacacccagg tatgcggaaa gagggcgttg ccgagcaatc cgatgcggtc gtagaaccgc
    30601 tccagccaat cggcggaagt catcggcgtc gccagaacgc ctcgaaacca ccatatcccg
    30661 accacgatga tgagaatgag caccagaaca agcgcccgtc ggggcgtgcg agggcaaatc
    30721 atcaccgaac cccacgccag caacaacccg agagcccccg gcaacgggat gaacgcgagg
    30781 aacagggcca gaaaaagcgg atagaacgtc cattcctcgc tgtagacgcg agccaatgcc
    30841 atcatcagcg gcaaaccgag caggatcagg gaccaactcg acaaaacaag gctctcgatg
    30901 aacttgacga tgaccagatc ccggggctcg accggtgcgg ccagcagata accaatctcg
    30961 ggccttttga agagaatccc atagcagaga atcgcgttcg agaaggccag catgcccatc
    31021 agagccagga acaggaagtg aaacatcacc ggtgtggcaa cgatgccttc gaggaccgac
    31081 ctgttcacca ccagcagcga ctgtaccaac aattcgtaga gagcggccca gatcagcagc
    31141 agtgacgcgg ccgtgcccag gaacttgagc ggccgatcct gaaaaacctg tcggagcgta
    31201 ttcctcagga cggccgtccg cacccggaca aggaacagca cgccgggcgt ggcggagacc
    31261 gtcgcgcaaa cgccgccggt ggcgggaacg tctcgtcgct cgctggtcat tgccttagca
    31321 tcccgaaaaa cacatcctcc gtcctcatac cgggatggtc tcggcctgcc cgtttccgtg
    31381 gtcggcggtc agcctcagga agatatcctc cagggattcg tcgcgacggg catgggctcg
    31441 gagttcctcg agggtaccgc aggccgtaag cctgccacga tgaatgatgc caatccggtc
    31501 cgcgacggcc tcggccacct ccagcgtatg ggtgctcatg aacaccgtgc atcctcgcga
    31561 ggtcatttcc cacatgattt ctttgaccgt gcggacggtg cgcggatcga gtccgaccat
    31621 gggttcgtcg atcaccaaga gcttggggtc atgcagcagc gcggcggcca agacggtccg
    31681 ctgtttcatc ccgtgagagt agctttcggt aagctgatcg aggaagctgc ccatctccag
    31741 tcgttcgatc aggccgccag cgcgagcctt tattgtggcg gcgctcagcc cgtacatgcg
    31801 accgacgaaa tcgaggaatt cccggccggt cagcttttcg tagagaaacg gctggtcggg
    31861 cacgtaggcc atccgggctt tcgcctggcg tccgtcgcgg cccatggcat acccgcaaac
    31921 gcggatgtcg ccgtcgtcgg ccaccagcag accggcaatc atcttgatcg tcgtggtctt
    31981 gcccgcgccg ttagggccga gaaaggcaaa cagctcgccc tcgcgaatct ccaaagaaag
    32041 ccggtcgacg gcggtgaaat cgccgtaccg cttggtgact tcgcgcagtt caatggctgc
    32101 gggcatgttc agttcccctg cggctccatc cgccccatgc tcttgtggct cttggcattg
    32161 tcgcgagctt cttcgttgta ggcctccatg atcaaggtga cgcgaccgac gccgggcaga
    32221 tcggcctcct gccgaaatac gcgacctttc tcatcgaccc aggccttgga agacggcggc
    32281 acggtttcca cgatgaaaca gtcgacggtg cggcccggct cgaaggggtg ggcaattgct
    32341 tctttgccgg tcaccctggc gaccaccgat ttgaattccg tccgcccgct caacaccgcg
    32401 gtcaacgggt caagcatctg catccgccat gactgtccga gacgaagggt gggcaggcag
    32461 gccaatggat tcaacgactc cccgatcatg cggctggcgg catagtcaag gggcacctcg
    32521 cgacggatcg gaccaaactg catttccacc gggaaataga tgccccgccg ctcgccgtgg
    32581 acgaagatct tctggtccag gaagccatgg acgtccagcc tgaaaccctc caatgctccc
    32641 tgccggtcaa aatcagcctg agactcgatt cggattgagg gaatcatttt caacggcaat
    32701 gccgggatgt tcgacagccc atcgagcagg accgtcccgt tgaccgacgc ggtctcgcgg
    32761 ttcacgcgaa cctcgctcca ggcggttcca aggcggcggt cccggctgtc gacgattcga
    32821 tactgttgga ggggctcatc aagtaacttg aactgctcca cggtgatgcg cggcgggtct
    32881 tgggcgatc
//
LOCUS       DAOTPW010000024        32831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1431_length_32831_cov_5.387479, whole genome shotgun sequence.
ACCESSION   DAOTPW010000024 DAOTPW010000000
VERSION     DAOTPW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 32831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32831
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1431_length_32831_cov_5.387479"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1037..1912
                     /locus_tag="P5316_03640"
     CDS             1037..1912
                     /locus_tag="P5316_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84079.1"
                     /translation="MSGEAIAESAGSQPTFELELVPGKEVSHWSGLPVWGEKEARERG
                     FELPPPLGVSGNVYSAKHNFDIPETKLGGPRGPLLDVGSLVRVTNVKIAETAWTSRFD
                     AWVLPFLNLYAIAGYVDGTADIGVRPAMLPIGPKLDIHLEYEGPTVGLGGTLAAGFNP
                     IKDRPTIVFGLADLNFTQTFLDFKRLVASLDAVDVVVFSMRLGVRERILQNSSLGDVY
                     VSLWGGAMYQGVQEIMTGRLEILDLDYRAKVEAVNPWNTIVGGRLEIGENIYLTVEVG
                     IGDRESLMLEASFRF"
     gene            2154..4604
                     /gene="hypF"
                     /locus_tag="P5316_03645"
     CDS             2154..4604
                     /gene="hypF"
                     /locus_tag="P5316_03645"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRR84080.1"
                     /translation="MASTVAVLKGEQPPAYELLGGAVLGFGGGAAYNTCVRTGSSSDV
                     EQERSRRRLAVQGRVQGVGFRPFVCRLANALRLGGFVGNDTHGAFIEVEGPASAIDEF
                     ERRLREELPPLARIAQLTGSPIASVGETTFRIIHSSSAGRQDAEITPDTATCADCLRE
                     LFDPADRRYRYPFINCTNCGPRYSIIRAVPYDRPNTTMRRFAMCPLCQAEYDDPANRR
                     FHAQPNACPVCGPRVWLSDGNGREIPGDAVRLGAAMLRDGAIVAIKGIGGFHLACRAD
                     QDEPVARLRVRKMREAKPLALMVATIEAASEIAEVDEAAVAEMSSPVRPIVLLPRKPQ
                     ASISRNVAPGNDCFGIMLPYTPLHHLLMAEGTGPLVMTSANPTDEPLCARNEEAMERL
                     ATIADAFLMHDRDIERRVDDSVVKLMTGPHRKLVMVPIRRARGLAPAPIHVPAESPLP
                     VLAVGAELKSAVCVLDGRQAVLSEHLGELENPAAFRNFTATIEQFKKLLRVEPRIVAC
                     DMHPDYAATRYAATLGLAVTRVQHHHAHIVSCMAEAGITGRVLGVSCDGTGYGTDGAI
                     WGCEVLACDEAEFQRVGHLRYFKLVGGDQAAKDAWRPAAGLLHETFGQDWIRVAGPFL
                     GRIEEQALKLVARRFASAGRIPWTSSLGRLFDAVAFLLDICDRNQYEAEAAMALEAVA
                     RAAAPTGPLAYNVQKPENDGRPAVCDFRPMVKELMARAADGPDAGTLARAFHEACAGM
                     LADVVEYAAARTGIRRAVLSGGCFANRLLLESLTDRLIQRGLTVFSHREVPTGDGGIA
                     LGQAVVAAERVRRGLVCV"
     gene            4595..4864
                     /locus_tag="P5316_03650"
     CDS             4595..4864
                     /locus_tag="P5316_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRR84081.1"
                     /translation="MCLAVPGRVLDIRTPESGAPMSTVGTVDFQGTRLEVGLAFTPEA
                     KVGDWVLVHAGYALSVLDEAEAMQTWTYLKAAGLADLPPELSGGQ"
     gene            4892..5977
                     /gene="hypD"
                     /locus_tag="P5316_03655"
     CDS             4892..5977
                     /gene="hypD"
                     /locus_tag="P5316_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006695319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRR84082.1"
                     /translation="MSHLSIEEMKERIADVARGLRPIQLMEICGTHTVALFRTGVKSL
                     LPPTIRMVSGPGCPVCVTSQGYIDAACEAAIRPEVTICTYGDMVRVPGRTGSLEKQRA
                     RGAQVVVVYSARDAVKYAADHPDRQVLFLAVGFETTAPITAAAVLDAQNRDLRNFFIL
                     SGHKLVIPAMVALLSAGDVPIDGFLCPGHVTVVIGSAAYRPVVEQFGKPCVVAGFEAG
                     QMLLGLLRLLEQIAFGESRVENVYGVAATEQGNLAARECIDRIFEPADTVWRAMGTIP
                     KSGLELRPAFRRFDAVAGLGLTIGEDYEPPGCRCGQVIQGRLLPTDCSLFGKACMPTT
                     PVGPCMVSSEGTCAAWYKYGGARFAER"
     gene            6042..7067
                     /gene="hypE"
                     /locus_tag="P5316_03660"
     CDS             6042..7067
                     /gene="hypE"
                     /locus_tag="P5316_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRR84083.1"
                     /translation="MTRNEPSGEEQIVLAHGGGGELTRRLIQERFLSRLSNPLLEPLT
                     DSAILSVSGRVAFTTDAFVVQPLEFPGGDIGRLAVCGTVNDLAVAGAIPRALSLAMVM
                     EEGLELALLDRVIRSIAQAASEAGVVIATGDTKVIERHGGDGLIITTAGVGDVREDAR
                     LGPDRLVPGDAVVVTGPIAEHGLAIMSVREGLGFDTQLTSDVAPLAGLVDAMFSSGAD
                     IKFLRDPTRGGMAGVLADLAEESGLTVEVIEESIPITPVARHTAELLGLDPLTVANEG
                     KVVALVAGEDAGRLLQACRNHPLGRQAAVIGRIVRRDPPLVELVTPAGGRRIIQRPYG
                     EDLPRIC"
     gene            7106..7480
                     /locus_tag="P5316_03665"
     CDS             7106..7480
                     /locus_tag="P5316_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRR84084.1"
                     /translation="MAIASEIVDCLLDIAGRYKAARIGEVEVEIGRMRQVVPEALEMA
                     FSAAVVGTVAEGATLKLREIDVSAVCNDCGAAYSPDIDVSFMCPSCGHANPRITAGDD
                     IVLRNITCDVDEEYRYEADPRD"
     gene            7458..8126
                     /gene="hypB"
                     /locus_tag="P5316_03670"
     CDS             7458..8126
                     /gene="hypB"
                     /locus_tag="P5316_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRR84085.1"
                     /translation="MKQIHVIESVLKAAETAAGENRRLLDQAGLLAINLMSAPGSGKT
                     TLLENTIPALGPDCPCAVLVGDLQTTRDAERLGPLAVETVQINTGKGCHLTPGQVAEG
                     LRRLDLSRFAFLFIENVGNMVCPAAFDLGEHLRVVLLSIPEGDDKVAKYPTLFQKADV
                     VLLTKVDLLDILDFDVSRVRQDLSRLNTRAPFFEISSKRGDGMDRWLAWLNDRRQARS
                     KASR"
     gene            complement(8633..10045)
                     /gene="thrC"
                     /locus_tag="P5316_03675"
     CDS             complement(8633..10045)
                     /gene="thrC"
                     /locus_tag="P5316_03675"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRR84086.1"
                     /translation="MAEKAYQICINPDCRETLSVDQVVFSCPRCGSLFDIAYDWDRLA
                     VPTSLEFFEHRWATKGLNAEGRLDFSGVWRFRELIPFVKPEQIVTIGEGRTLLEENNR
                     LAAEVGMKPGRLFLQYEGLNPSGSFKDNGMTAAFSLARALGRTRVACASTGNTSASLA
                     MFANKTDPPMQGIVFIGSGKIAYGKLSQALDYGALTLQIEGDFDACMRRVKQISDKLG
                     IYLMNSMNPFRLEGQKAIMYRVLEARSWEVPDWIIVPGGNLGNCSAFGKAFLELHRLG
                     LIKRIPRLAIINATGANTLYRLHEKEGVRWNGGRYDQEKVKNLYAEFDSTGYLPHTVA
                     SAIEIARPVNLPKALRALEVMNGLVREVTDDEILEHKALVGRYGFGCEPASAASVAGL
                     HMLLREQIISPDEQVVCILTGHELKDPDATVKYHTGIDMKAIPPPPPVPPKGRFANTP
                     RKVADDLEAICAALGEKLPN"
     gene            complement(10198..11103)
                     /locus_tag="P5316_03680"
     CDS             complement(10198..11103)
                     /locus_tag="P5316_03680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRR84087.1"
                     /translation="MSWRDRPYAADDSQPELRIQLRRPSTIVMWLIVINVVVFLFHSL
                     SQNWSQSYSVLTFGLSLDGVLSLRLWQPLTYMFMHADTWHLVFNMIGLYVFGSEFERS
                     FGQHRFLQFYLSCGLLGGLAYLGLGWADETHVGIPLVGASGAVYGLLMAAIIFFPHIQ
                     IILLVFPMPIRVFGLIVLAIAVLTVLSPSSSFNRGGELCHAAGAMAAIVVFYAWGVMP
                     RIRLGFGRGVTILPGSGDRRSKGGEGAWEKKQRMLAEEQAEVDRILAKVHDQGLASLT
                     RKEKKILAQATRRQRERDEEAQRRA"
     gene            complement(11100..11624)
                     /locus_tag="P5316_03685"
     CDS             complement(11100..11624)
                     /locus_tag="P5316_03685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRR84088.1"
                     /translation="MREVLLQTRRFRVERLEYHIPGQGMVRRELVVHPGAVTIVPLLD
                     SETVVMIRNYRFAAGAELLELPAGTLEPPEPPMECAARELEEETGYKAAQLERLCEFY
                     TTPGFTDERMYVFVGTGLTPTRQRLEATEQIRIEIMPLNDALAATTDGRIVDGKTIAS
                     LHVYHHRYRSGDKP"
     gene            11841..13034
                     /locus_tag="P5316_03690"
     CDS             11841..13034
                     /locus_tag="P5316_03690"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRR84089.1"
                     /translation="MPRESLPLEPSAGAEAKRVCIMGSTGSIGRSTMDVVRSLGPPYR
                     VTGLCAARRWRELAAQAAEARPSAVALAQTDHAKELKPLLNGSTELLVGPEGLVELAA
                     RPDSDFVVAAIVGAAGLPSTIAAVRAGKRVGLANKEALVVAGSILIPLARKSGACLLP
                     IDSEHSAIFQSMTAGRPSEVKRILLTASGGPFRTWSGPEIAQATVEDALRHPTWKMGP
                     KITVDSATMMNKALEIIEARWLFGLPVESIEVLVHPESIIHSMVEFCDGSVIAQLGTP
                     DMRTPIQYSLTYPNRVPGCATRLDFATIRRLNFEPPDLERFPALRLGYEVARAGGTAG
                     AVFNAANEAAVEAFLNRRIPFGRIVQLIEEVLGRHAVSPSPDLDELLEADAWARREVN
                     ECLKD"
     gene            13019..15412
                     /locus_tag="P5316_03695"
     CDS             13019..15412
                     /locus_tag="P5316_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRR84090.1"
                     /translation="MLEGLIGLSAVGQAVPLLGSVMGTLFGYVLIILGFSFLIFVHEL
                     GHFLAAKGCGVRVDRFAVGFGRALCSYRKGLGFRWGSSEEQYLERLREHVERKRQAEL
                     QFKEKTSVTDEELARAAQELKLGETEYRFNWMPLGGYVKMQGQEDFEIDKSGEIEVKN
                     DPRSFLNKSVGRRMIIVSSGVVMNAIFAGILFMITYMIGKPAIPPIVGGVMPGWPADR
                     AGLLPGDRIIEVNGERITDFDVLRMATILADPNKPMSIVFERQEHKDGPPVRNTVEIQ
                     PNMDDVLGVLVLGIRSGGNNIIAGVWPDPKLAPEEQPQIGDEVLEADGRKITNSQDLL
                     AVLQRRQGDYARLVVRRRLEDGTNVTREVKARALRRLLPRGEGKESDQELLGFMPRTC
                     VASVEPNKRADLAGLKAGDVILKWGEQPDPTASEVRDAIAANPERDIRVRVLRHEGSQ
                     PPQHVWLVVRPAAGALGRGRPHVGMNMEADAEGPPFVAGIALEVREGVPTAAAPLKEK
                     MPRGSLITSINGEPVATWGEVVDRFFKFAGGEVQVAWRTPEGLEQRGSIRVPATLGTI
                     FPLPGDHVIVRIDDRRTAEVKIRDQYTPVPVGTWLGASHALEMCIGRKIEIEHRGMQG
                     NAGHTELVEVTREMLDTWPMRIAYDRVTLETYALREHFREPNPIKAMMIGARQTYYFI
                     ETVYLVIQRMVVDRTVGFDTVAGPIGIVKAGSDIAQENFSSLLYFLALISANLAVINF
                     LPLPIFDGGVMVFLIIEKIKGSPIPLRVQVVTQLVGLALIVTIFVYVMLNDIQRIFG"
     gene            complement(15433..16365)
                     /locus_tag="P5316_03700"
     CDS             complement(15433..16365)
                     /locus_tag="P5316_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84091.1"
                     /translation="MVDVEQSGTSLPSQRNAPLPGRGRRPTIAIIALLVALWCLAMWY
                     RAEIRAYWWARKLAQSSSPQDIHYYLLCLASTEESAFGPAKRLVRNTDPRVRRLGVAV
                     LDNCRGQEVEHVLLDLLNDPDDEVADAAAISLTDPRRGTAMALIPELEKIVARGGKAA
                     RHAIVALQRIPGEQAEMILVDVLSEASEPDLLGQVADSLGIIGSRTAVPGLIALLDDT
                     RSLGSMLYSERSAAKALADARAQFAARGINAESVTVARAHTVAEAALRSLTMIAGGTS
                     PRAASGPANTRPAGMLREQWETWWKSGETPQPGG"
     gene            complement(16475..17224)
                     /locus_tag="P5316_03705"
     CDS             complement(16475..17224)
                     /locus_tag="P5316_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRR84092.1"
                     /translation="MALVGDWRWISDGPADGPVNMARDEAILLSVSEGGAPPTLRFYR
                     WSPPTVSLGYFQSYSDYAALPPPAGGLPVVRRLTGGGAILHDLELTYSLVLPSNHPLL
                     PACGAAGLYDVVHDAFARFLTNLGVSVIKGPSASGGASHRGPFFCFDRHNRFDLLTDG
                     RKIMGSAQRRLRTAILQHGSLILDNRFQQQCCASVAEYAPVVINTWLPRLVEEITRAP
                     AGKPAKLSNGELETAERLVFKYRDEQWTRRR"
     gene            17898..18302
                     /locus_tag="P5316_03710"
     CDS             17898..18302
                     /locus_tag="P5316_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRR84093.1"
                     /translation="MTALFVAVLVMAPGVGEWEPVVMDVSAYCPCPLCCGAHASGITA
                     SGKPAVGKLIAAPSSYAFGTEMRVPGYGTAKVLDRGGAIKSAGEWVRNVKIGDEKVAD
                     VKLQHDRIDLLFPTHAEALKWGRQTLTVWVRR"
     gene            complement(18483..20003)
                     /gene="gcvPB"
                     /locus_tag="P5316_03715"
     CDS             complement(18483..20003)
                     /gene="gcvPB"
                     /locus_tag="P5316_03715"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRR84094.1"
                     /translation="MSAEPIGATRNKDALGRAALPPPERLVFELSAPGNSAVSLPAGD
                     TPAIDAASVIPPELLADEPPPLPEIGELDLVRHYTRLAHRQFSVDGNFYPLGSCTMKY
                     NPKVGERVSSLPGFTELHPLQPAEDVQGILELLYHLRIFLAEIAGLAEVTLQPCAGAH
                     GEMTGLKIISAWHRSRGEARPKVLAPDTAHGTNPASCAVCGRQIVTVASRRDGRVDLD
                     DLRRKVDDRTAALMITNPNTAGLFDPQIAEIADILHTKGALLYLDGANMNAILGIARP
                     GDFGVDVMHFNLHKSFGTPHGCGGPGSGPVAVAERLRGFLPGPQVVRGEDGRYDWAAP
                     GPQSIGRVRWFYGQIGILIRAYAYIRMLGPEGLREVSEKAVLSANYLAARLRDHYRFP
                     FDPPYAHEFIMVPEFREQGVTEIDIAKRLIDHSFHPPTMSWPVHHCLMVEPTESESLA
                     TLDHFADVLIQIAREAREEPEVLRQAPLTMPVHRLDEVTAVRKPDLRRRSEQDSSK"
     gene            complement(20032..20415)
                     /locus_tag="P5316_03720"
     CDS             complement(20032..20415)
                     /locus_tag="P5316_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84095.1"
                     /translation="MGYDVEFVQLTMPAGTKFPVEPESAARLVKKAAPFADVQVVRAA
                     LLKLEGCRPGPKDAVDYLGRGMSYARIFPRHAAIYVENNCNAADLIRILVHLQKDFPS
                     LLILDMQSKQLHTAESYKAWWGKPL"
     gene            complement(20434..21771)
                     /gene="gcvPA"
                     /locus_tag="P5316_03725"
     CDS             complement(20434..21771)
                     /gene="gcvPA"
                     /locus_tag="P5316_03725"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRR84096.1"
                     /translation="MRFTQLTERDVQHMLATIGIGHVGELFGGIPDRLRLDRPLDLPV
                     PLTELELLADLERLADRNQGCNRQVCFLGAGIYDHFVPTVVDALACRSEFVTAYTPYQ
                     PEAAQGALQALYEYQTLICELTGMDVSNSSLYEGASAVSEAVLMARSINGRGRVVLPM
                     TVHPHVRETLQTYLRDLPMEIATCGMQDGRTSEDEFRSVVDGDTTAVVIQTPNFFGCI
                     ERLDRLAEIARAAGSLVIASVDPLSCGLLKRPGQLGADIVVGDGQPLGIPMSFGGPTL
                     GFMACRDEFMRKLPGRLVGATTDLSGRRAYCLTLQTREQHIRRERATSNICTNQGLLA
                     IRAAIYLSAVGPHGLTRIARLCLEKAHYAAQAIAAVDGFKLRFASPFFKEFVVQSDRE
                     VPRVLEQCRRAGILAGVPLGRWYPELADCFMVACTEKRSKKEIDHLVGALGKR"
     gene            complement(21787..22176)
                     /gene="gcvH"
                     /locus_tag="P5316_03730"
     CDS             complement(21787..22176)
                     /gene="gcvH"
                     /locus_tag="P5316_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRR84097.1"
                     /translation="MSPTPSDRKYTKTHEWCLAEGDVVTIGITQFATDQLADITFVDL
                     PSIGTRVTAGQACGEIESVKATSELYTAVSGEVIEVNKALGDAPELVNNDPYGQGWMV
                     KIRAANLSELDALMDAAAYDRLLAAAS"
     gene            complement(22217..23308)
                     /gene="gcvT"
                     /locus_tag="P5316_03735"
     CDS             complement(22217..23308)
                     /gene="gcvT"
                     /locus_tag="P5316_03735"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRR84098.1"
                     /translation="MRRTPLYDLHTRLGAKMVDFAGWEMPLLFTSIIEEHRHTRSAAS
                     VFDVSHMGRIELTGPDAESLLERICTRRVGKAVVGQSLYSHICREDGGILDDVIVSRF
                     EDHWLVVCNASNRDRIVAWLRRHASGSDLDIKDRTEETMMVAIQGPLAVELVSRLLPI
                     AVADLRRYHFCSGNYMFTPYAIFRSGYTGEDGFEIILPANVAQLVGSYLVGDEVASNT
                     IKPAGLGARDTLRLEAGMPLYGHELHEHTDSLSAGLEWCVDLGKDFIGAEALRRISQA
                     GPSRRLVGLELTGRRIARPHSAVLSENRPVGEVTSGTFSPTLERSIAMAYVDAAHAQE
                     GRPLTVDLSGKQADATIVRLPFYKQKTGT"
     gene            complement(23390..24502)
                     /locus_tag="P5316_03740"
     CDS             complement(23390..24502)
                     /locus_tag="P5316_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84099.1"
                     /translation="MRVDVHCHIGQRRRLCGDEGRFSFEAPGQYAPCDSYFSDVMYYG
                     VFMRLARLHFGLGGGRTSEQEFDARVEHILLDHILGAENVDRVVLLAFDQYHTDEGRP
                     LGSRRHLWQHGSDLYVSNTYARQLWLRHPRRILFGASIHPYRRVGRKTAMDMLEEVAA
                     AGAVLVKWLPLSQNIDGQDPRTIDFLRRAASLKIPVLIHCGCEAALGNMHPRFKDPAP
                     WLRTLRQLRQEQCPPTVIIAHVASPPLWPVTSARTLHTLLDALTGEFADAPLYADLAG
                     MTMINKAVWLKKLARMPEIHHKLVHGSDFPIPPFPIVFWPQLGRQYRTIRQLKSWLDQ
                     DIAVKTALGFPESIFDRAGELLAERIRFADALAGRV"
     gene            complement(24523..26844)
                     /gene="rnr"
                     /locus_tag="P5316_03745"
     CDS             complement(24523..26844)
                     /gene="rnr"
                     /locus_tag="P5316_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRR84100.1"
                     /translation="MPAKTCEPVKESRINTTNDLTNRILQHVSRKDYRPQRARRLAQS
                     IGIEEKEYGEFRAAVKSLMKAGRVILGGRNCVMLPSAVGVIIGTFRGNPRGFGFVVPA
                     NPTDHGDLFIPPGAAGGAITGDTVEARITRRGKRGDSALVEGEVTRIIERGQSRFVGE
                     LIRQGEQWLMIPDGHALHAPVIVGDVGSTRARTGDQVVVELTEFPSATRAGRGVIIEV
                     LGRRSDPGVDTLSIIHQYHLRHAFPPEVADDVRHVVRKYALETELGSREDLRREVVVT
                     IDPDDAKDFDDAISIRRSARGRYELGVHIADVSAFVRPDSPLDVEARLRGNSVYFPRH
                     VIPMLPEVLSNGLCSLQEGEPRLAKSVFIEYDTKGRRIASRFANTVIQSHKRLTYREV
                     TRVLAGETRGVNPQVVALLKSMEHLARLIQKRRLAAGMIVLDLPGVELIVNDGDEVID
                     VIPEDTAFAHTIIEMFMVEANEAVAELFHRLGVPHLRRIHPEPPAESQNKLTGFLRVL
                     GKPVPKRLERSDMIRLLDSVRGQPEAFAVNLAVLRSMAQAEYSPAMIGHFALASRHYT
                     HFTSPIRRYPDLVVHRLLQQYLEHQLDSPQGKSRAPSSEELAVLGAHCSFTERNAEAA
                     EREIRLVKILRFLEKRVGDIEQGVVTGVANVGVYVQLLKYRVDGLVRFADLPDDWWDI
                     DVRSGCVVGQRSRKRIAIGDAVNVQLAGVDLSARELDLVLTADSLKTGTGRARPSVAK
                     RGEPDRGRKAARVERKTVRRRDSRRPRRIGRRR"
     gene            complement(27031..27372)
                     /locus_tag="P5316_03750"
     CDS             complement(27031..27372)
                     /locus_tag="P5316_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRR84101.1"
                     /translation="MASQYAGLKSPPARKREIPSRAAASSRRAGLQAAAFEAITYTPP
                     PWKKLKRFLLRGLTTKERLIITLKYCERLTFAEIGVVLALPEKQVAALHRDIVRRVRR
                     WVLSDDYDSWT"
     gene            complement(28095..29765)
                     /locus_tag="P5316_03755"
     CDS             complement(28095..29765)
                     /locus_tag="P5316_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HRR84102.1"
                     /translation="MSEKAVWFNLDSLIDPNITGGRVPSYKEGQPEWAKGHLALEDNG
                     WKNQVMMYALQGLVNRTGPRLMYDTQFWNWKPADQTWRDYYARAKGIEFETLADVFEV
                     IDRFREAFKGLVVHDPSVEQSLYVACVLAGLEDLLPVKPDIAEKLTARYGDLTIAYDL
                     VGRWKDEFEPLDYAIDNLLPRCQPGMIYSVDNLWTGMSIHTLDLAVARRAFIFRCSTK
                     SEYAEDNKRIWRIHSYAGPNCGVYGWGEPEDRYCQMASLNSNYIMCTEAPNLSFHAQV
                     PVEMKTFRQKSHVDKSQLRLEEDKYYVAFMTTEGDALKIHMCFQGGAWHDPHRGRVPI
                     NWGFQPRMLDVAPAMAEYYYSTMTDKDYFFCGCSGAGYTYPNWMPEPDEFFRESDKFM
                     ERADLQTMDCWIHFSRPVYERYAELSPHTEAFILPCGPGQVKMTRAGTPVILRYSGLH
                     YFPSEKTAEDMAEAIQKAAADILHKPAFITVFAVPDAKGNTSAQNGFCPEDYVRVAEI
                     LGDREYKIVTLEEMAWAAREFARKYPDRVNRPQSRERQTRVGVNVIQS"
     regulatory      29915..29978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            30091..30480
                     /gene="crcB"
                     /locus_tag="P5316_03760"
     CDS             30091..30480
                     /gene="crcB"
                     /locus_tag="P5316_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRR84103.1"
                     /translation="MVKLVLVFLGGGLGSLCRYGLAGWGQRLVNGSFPVGTLLVNVLG
                     CFVIGFLNYLFNGGAYLIRPEYRVALTIGVLGGFTTFSTFGWETFAMANDGQGLRAVM
                     NLLLSVTLGFSSVLVGYRLAEKWFGAG"
     gene            30555..31802
                     /locus_tag="P5316_03765"
     CDS             30555..31802
                     /locus_tag="P5316_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRR84104.1"
                     /translation="MTDVWFTAALWVGLALVATLLSIWLRVATALSEIVVGTVAQLII
                     GAVAGVAALGAQEPWIKFLSGTGAIVLTFLAGAELDPTVFRARWKEATVIGLIAFFTP
                     FLGCAAVAYYVLHWDSKASWLAGVALSTTSVAVVYAVMLELGFNKTDFGKAVLAACFI
                     NDLGTVVALGLIFSPFTIRTGVFVIASVAVFAIIPFLTPWFFRRYGNRPSELEAKYLL
                     LLLFGMGALAAWADSEAVLPAYMIGMVLAGTVGKDHVLVRRLRTLTFGLLTPFYFIRA
                     GSFVSVPALIAAPVTFMILLGAKVVSKCLGVYPATKAFRYEYRQAAYTTLLMSTGLTF
                     GTISALFGLTHNVITQSQYSYLVAAVVASAVIPTLIANAFFIPYDLLPKRTRATMPDI
                     RPAGSREMTATAVLDGESVLPET"
     gene            31814..32227
                     /locus_tag="P5316_03770"
     CDS             31814..32227
                     /locus_tag="P5316_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRR84105.1"
                     /translation="MKTILAANDGSESAKRAFQFALQLAKAFGSSVVVVSVAQPSEPP
                     TSVETPALLDAATEHFEKDFVAFREAAQAAGVPLETDVVVGHPADQIVHQAVQRQADM
                     IVMGHRGKSRFQRWLLGSVSKRVLSYAPCTVTVVR"
     gene            32231..32584
                     /locus_tag="P5316_03775"
     CDS             32231..32584
                     /locus_tag="P5316_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HRR84106.1"
                     /translation="MRLEGEAQLLRVFIGEGDKWHGVPLYEAIVMKARELHLAGATVL
                     RGPMGFGAHSRLHTVKVLRLSQDLPMVIEIVDTQEKISTLLPHIDQMVTEGLVTLEKV
                     HVIKYLANGGDARKE"
     gene            32613..>32831
                     /locus_tag="P5316_03780"
     CDS             32613..>32831
                     /locus_tag="P5316_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84107.1"
                     /translation="MSSDKSSERRACSRDREFVREQLLRPGRYFGCEHDAMGVCFLGR
                     EAFSPAESEFRRAVWLNPYDATFRTHWAI"
BASE COUNT         6068 a         9959 c        10412 g         6392 t
ORIGIN      
        1 gggaaagggg cggtagcgat cggttggtcg gagacgctgg gacttggctc gactgaggcc
       61 tgaaaactga caactgattg ctttccgtcc cccgtgcatc gggcaaaatg cggggatagt
      121 atggaagctt ccggtggcac agtgggtgga gcatttcgga tctgagaggc gggtgggatc
      181 aagagtggga atcgaacggg tggaggacgg tgggcgaatg ggaaaacgaa ccaatgcaaa
      241 aacgaacctg tcccgctttc cggattcgcc ccgtgctcgt tgacatgttt ggactcgtca
      301 aagccgcgct gatccggccg ttcgatcatc tcaccactca tgaccgcagg tctccgaatg
      361 cccttgcgac agtatacgta gaatacctct tgctgccagc ctgatgcgac gccacccccc
      421 accagaccgt ctgaaacctg gggaggacga gaccttggcg gttgtccgcg tctttggacc
      481 tgtgcggctt cggctttcca tcagacaggg gcagtttgcc actattccag tgacagcata
      541 tcccaaggtg tacgggatgc caataaggga aaagaagccc tcagacgtca gcaatctgcg
      601 gtcaacggga gtctgttggc acaatggcct gttcgattac ctgactgctc aaccgttcgg
      661 cgatgaacaa gggctccatt cccgccgctg agcgagtggc tacgtggctg gcgcggggcg
      721 aatgtggaat gctgaatgat gatggggcgg tgggcgagcc gggtaacagc ggcggtctct
      781 gcggccttgc gagtgattcg ggggttgtcg gttggaggac cacggggatg gattcgacaa
      841 gcttaccccc gacgaactgc gtggctcgtc gaggcttggg cacggcgtgt ggtgggcgcg
      901 gtgcgtcgca ggcggtagga tatgaacccg ataccaccgc gatggtattc tccagagtgg
      961 aacgagcatc aggagacgca gcatcggccg attcagatcc gttttcgcgt ccgtgttcct
     1021 caccttgggt tggctgatgt ctggggaggc catcgccgaa tcggccggca gccagccgac
     1081 ctttgagctg gagctggttc ccgggaaaga ggtgagtcac tggtccgggt tgcccgtctg
     1141 gggtgaaaaa gaggcccgcg agcgcggttt cgagctgcca ccgccactgg gcgtctcggg
     1201 caacgtctac tcggccaaac acaacttcga cataccggag acgaaacttg gggggccacg
     1261 aggtccgttg ctggatgtcg gcagcctcgt tcgcgtcacc aatgtcaaga tagcagaaac
     1321 agcatggacc tctcgctttg acgcatgggt cctcccgttc ctgaacctgt acgcgatcgc
     1381 cggctacgtg gacggaacgg ccgacattgg agtacggcct gccatgctcc cgatcggccc
     1441 caagctcgat atccacctgg agtacgaagg gccgacggtg ggtctcgggg gcaccctggc
     1501 cgccggcttc aacccgatca aggatcggcc cacgatcgtg ttcggcctgg cggacctgaa
     1561 cttcacccag accttcctcg acttcaaacg cctggtcgca tccttggatg cggtcgatgt
     1621 cgtggtgttc agcatgcggc tcggggtgag ggagcgcatc ctgcagaatt cgtcactcgg
     1681 cgacgtgtac gtttccctgt ggggcggcgc catgtaccag ggcgtccagg agatcatgac
     1741 ggggcgcctc gagatcctcg acttggacta ccgtgcgaaa gtggaagccg tcaacccgtg
     1801 gaacacaatc gttggtggcc gcttggagat aggcgagaat atctatttga cagtcgaggt
     1861 cggcattggg gaccgcgagt cgctgatgct ggaggcctcg tttcgattct gagatgcggc
     1921 aggccgtaga gtctacctcg ggagaagcat cggggctcct gctgcgctct gggagctacg
     1981 caggacgagt gatcgttgct cccccacccg gcgccgcgcg agatagggga aggtagacag
     2041 gagagcaggg gacagttgag caggagaaag agagctgaca gtcgaggagg gtccgcgagc
     2101 gcggaccgct ataaggagac tgctgcttcc tcccgccttc ggcgggcttc ggcgtggcga
     2161 gcacggtcgc ggttctgaaa ggagaacagc cgccggctta cgagctgctg ggcggggctg
     2221 ttcttgggtt cgggggtggg gcggcataca atacttgcgt gaggactggc tcatcgagcg
     2281 atgtcgagca ggagcggtcg cgccggcggt tggccgtcca ggggcgtgtg cagggagtcg
     2341 gcttccggcc cttcgtctgt cgactggcga atgcccttcg gctggggggc ttcgtcggca
     2401 acgacaccca tggggcgttt atcgaggttg aaggccccgc ctcggcgatc gacgagttcg
     2461 aacggcgatt gagagaggag ctgccgccgc tggcccgtat cgcacaattg accggcagtc
     2521 cgattgcgtc ggtcggcgag acgacatttc gtatcatcca tagttcgagc gccggccggc
     2581 aggacgccga gatcacgccc gacacggcca cctgcgcaga ttgcctgcga gaactgttcg
     2641 atccagccga tcggcgatac cgctatccct tcattaattg cacgaactgc ggcccccggt
     2701 actcaatcat ccgggcggtg ccgtacgacc ggcccaacac caccatgcgg cggttcgcca
     2761 tgtgcccgct gtgccaggcc gaatacgacg acccggcgaa tcggcggttt cacgctcagc
     2821 cgaatgcgtg cccggtgtgc gggccgcgtg tgtggctgag cgacgggaac gggcgcgaga
     2881 ttcccggtga cgccgtccgg ctgggcgccg cgatgcttcg tgacggggcg attgttgcga
     2941 tcaagggcat cggcggattt cacctcgcct gtcgggctga tcaggatgaa ccggtcgccc
     3001 gtctccgcgt gagaaaaatg cgcgaagcca agccactggc gctgatggtc gcgactattg
     3061 aggcagcaag cgagattgct gaggtcgatg aggctgcggt cgccgaaatg agcagcccgg
     3121 tacgcccgat tgtgcttctg cccaggaagc cgcaggcctc gatcagccgt aacgtcgcgc
     3181 cgggcaatga ctgtttcggt atcatgctgc cgtatacccc gctacaccac ttgctgatgg
     3241 ctgaaggaac aggaccgctt gtgatgacct cggccaaccc caccgatgag cctctgtgcg
     3301 cccgaaacga ggaggcgatg gagagactcg cgaccatcgc cgacgccttt ctgatgcacg
     3361 atcgcgacat cgaacgccgc gtcgacgatt cggtcgtcaa gttgatgacc ggaccgcatc
     3421 gcaagctggt gatggtaccc atccgcaggg ctcgcggcct ggccccggcg cccattcacg
     3481 tgcccgcgga atcgcctctt cccgtgctcg ctgtgggggc cgaattgaag tccgccgttt
     3541 gtgtcctcga cggccggcag gccgtcctga gcgagcacct tggcgaattg gagaatcccg
     3601 ccgctttccg caatttcacc gccacgatcg agcaattcaa gaagctcctc cgcgtcgagc
     3661 cacgaatcgt cgcctgtgac atgcacccgg actatgccgc gacccgatac gccgcaaccc
     3721 tgggacttgc cgtgactcgg gtccagcatc atcacgccca tatcgtcagt tgcatggccg
     3781 aggcaggcat caccgggcgc gtgctcggcg tttcctgcga cggcaccggc tacggcaccg
     3841 acggtgccat ctggggctgt gaggtcttgg cctgcgacga ggcagagttc caacgcgtcg
     3901 gccacttgcg atacttcaag ctggtgggcg gcgatcaggc ggccaaggat gcctggcggc
     3961 cggcggcggg cctgctgcac gaaacattcg gccaggactg gatccgggtc gccggcccgt
     4021 tcctgggcag aatcgaggaa caagccctga agctcgtcgc tcggcgcttc gcctctgccg
     4081 gccggattcc ctggacgtcc agcctcggcc gactcttcga tgccgtggcc ttcctgctgg
     4141 acatctgtga tcggaatcaa tacgaagcag aggcggccat ggctctggag gccgtggcaa
     4201 gagcggcggc gccgacaggc ccacttgctt acaacgtcca gaagccggag aatgatggcc
     4261 ggccggccgt ctgcgacttc cggccgatgg tcaaggagct gatggctcgc gcagccgatg
     4321 gcccggatgc cggcactttg gctcgggcat ttcacgaagc ctgtgccggc atgctggccg
     4381 atgttgttga gtatgccgcg gcccggacag gtatacgtcg tgccgttctg agcggcggct
     4441 gtttcgcgaa ccgactgctg ctggaatcgc tgaccgaccg gttgatccag cgaggcctga
     4501 cggtcttctc acaccgggag gttccgacgg gtgacggtgg tatcgccctt gggcaggcgg
     4561 tggtcgccgc tgaacgcgtc agaagaggtc tcgtatgtgt ctagccgtgc ctggcagagt
     4621 gctggacatc cgcacgcccg aatcaggcgc gccaatgtcg accgtcggca ccgtcgattt
     4681 tcagggcacg cgcctggagg tcggtctcgc cttcacgccg gaggccaagg tcggcgactg
     4741 ggtgctggta catgccggtt atgccctcag tgtgctggat gaggccgaag ctatgcaaac
     4801 ctggacgtat ctgaaggccg caggcctggc cgatctgccg ccggagttgt cgggcgggca
     4861 gtaacgtcgt caacctcgac tgtgagctga tatgtcgcac ctgagcattg aagaaatgaa
     4921 ggaacggatc gccgacgttg ctcgaggatt gcggccgatt caactcatgg agatctgcgg
     4981 cacccacaca gtggctctct tcaggaccgg cgtcaaatcg ctccttcccc cgacaatccg
     5041 catggtcagc ggcccgggtt gcccggtttg cgtgacctcc cagggataca tcgatgccgc
     5101 gtgcgaagcg gccatccgcc ctgaggtcac aatttgcacc tacggcgaca tggtgcgcgt
     5161 cccgggcagg accggcagcc tcgaaaaaca gcgggctcgc ggtgctcagg tggtggtcgt
     5221 gtattccgct cgtgatgcag tcaagtatgc cgctgatcat cccgatcgtc aggtgctctt
     5281 cctggccgtc ggtttcgaga cgaccgcccc gatcacggcc gccgcagtgc tcgacgccca
     5341 gaaccgcgac cttcgcaact tcttcatcct gtccggtcac aagctggtca tcccggccat
     5401 ggtcgcactg ctctcggcgg gtgacgtgcc gatcgacggc tttctctgcc ccggccatgt
     5461 aacggttgtg atcggttcgg cggcttatcg gccggtggtc gagcagttcg gcaaaccctg
     5521 cgtggtcgcc ggcttcgagg ctggacagat gctcctgggc ttgttgcgtc tgctcgagca
     5581 gatagccttc ggggagtcga gagtcgagaa tgtctacggc gttgccgcga ccgaacaggg
     5641 taacctggcc gcccgcgaat gtatcgatag gatctttgag cccgccgata ccgtctggcg
     5701 ggccatgggc acgattccca aaagcggcct tgagcttcgc cccgcattca ggcggtttga
     5761 tgctgttgcc gggttaggcc tgaccatcgg tgaggattat gagccgcccg gttgccgatg
     5821 cggccaggtg attcagggca ggctcctgcc caccgattgc agtctatttg gcaaggcctg
     5881 catgccgact acgcccgtgg ggccgtgcat ggtcagttct gaaggcacgt gtgcggcttg
     5941 gtacaaatat ggtggagcgc gctttgcgga acggtgatga aggccatatg tacgccgggc
     6001 cgtgggcatg gcgcccggca ccttttggac aagcatgacc catgactcgg aatgagccat
     6061 caggagagga gcaaatcgtc ctggcccacg gcgggggcgg cgagctgacg cggcgactga
     6121 tccaggagcg tttcctgtcc cgcctgtcca atcctttgct ggaaccgctg accgatagcg
     6181 ccatactctc ggtttccggc cgggtcgcct ttaccaccga tgcctttgtg gttcagccgc
     6241 tggagttccc cggcggagac atcggtcgcc tggcagtctg cgggacggtc aatgatctgg
     6301 ccgtggccgg agcgataccc agagccttga gcctcgccat ggttatggag gagggccttg
     6361 aactggccct gctggatcgt gtgatacgat cgattgccca ggccgcgtcc gaagccggcg
     6421 ttgtaatcgc caccggcgat acaaaagtga tcgagcgaca cggcggcgac ggattgatca
     6481 ttaccaccgc gggagtcggc gatgtgcgag aagatgcccg tcttggccct gaccggttgg
     6541 tgccgggtga tgccgtggtc gtgacgggcc ccatcgccga gcacggcttg gcgatcatgt
     6601 cggttcgtga ggggctcggc ttcgacacgc aactgaccag cgatgtggcg cccctcgccg
     6661 gcctcgtcga tgccatgttt agtagcgggg cggatatcaa gttcctgcgc gatccgacgc
     6721 gaggcggcat ggcaggggtg ctggccgatc tggctgagga atccggcctc accgtcgagg
     6781 tgatcgaaga gtccatcccc attacgcccg tcgcacgcca caccgccgaa ttgctcggcc
     6841 tggacccgct gacggtcgcc aacgaaggca aggtcgtggc tctcgtcgcg ggagaagacg
     6901 cgggccggtt gctgcaagcg tgccgcaatc atccgctggg ccgtcaggcg gcggtcatag
     6961 gccggatcgt tcggcgcgat ccgccgttgg tcgaactcgt cacccccgca gggggacgaa
     7021 gaatcattca acggccctac ggagaggatc tgccgcggat ctgctgaccg cctcgggcat
     7081 tcgagaacgc gttgcgatgc acgaaatggc cattgcttcc gaaatcgttg attgcctgct
     7141 cgacatcgca gggcgctaca aggccgcgcg catcggcgaa gtcgaggtcg agattggccg
     7201 catgcgccag gtcgtccctg aggctctcga aatggccttc agcgccgccg tcgtggggac
     7261 cgtggccgaa ggggcgacct taaagctccg ggaaatcgac gtttcggcag tgtgcaatga
     7321 ttgcggcgcc gcgtacagcc cggacatcga tgtcagcttc atgtgtcctt cctgcggcca
     7381 tgccaacccc cgcattacgg ccggtgatga catcgtcctt cgcaacatca cttgcgacgt
     7441 ggacgaggag tatcgatatg aagcagatcc acgtgattga gagcgtcctt aaggccgccg
     7501 aaacggcggc cggcgagaat cgccgtcttc ttgatcaggc cggcttgttg gcgatcaatc
     7561 tcatgagcgc ccccggcagc ggcaagacca ccttgctcga aaacaccatt ccggccttgg
     7621 gccccgactg cccgtgtgcc gtgctggtag gcgatcttca gaccacgcgc gatgccgagc
     7681 ggctcggccc tctcgccgtt gaaaccgtcc agatcaatac cggcaaaggc tgccacctca
     7741 cgcccggcca agtggccgag gggttgcgcc gcctcgacct gtcccggttc gcgttcctgt
     7801 ttatcgaaaa cgtcggcaac atggtttgcc cggccgcgtt cgatttgggc gaacacctgc
     7861 gcgtcgtgct cctcagcatc cccgagggcg acgacaaggt cgccaagtac cccacgctct
     7921 ttcagaaggc cgacgtcgtg ctcctgacca aggtggattt gctcgacatc ctcgattttg
     7981 acgtctcaag agttcgccag gatctgtcgc ggctgaatac ccgagccccg ttctttgaaa
     8041 tatcaagcaa gcgcggcgac ggcatggacc gctggttggc atggctcaac gaccggcgac
     8101 aggcacgatc gaaagcaagt cgataggccg tgcaagtgcc cgcgtccccg catgggcagg
     8161 tgcaggacgc agggtctgcg cctgtggacc cgttcggacc tctgcactgc catggcgtcg
     8221 ctccgtctcg accgcacgtt ccggagcatg cctcatgttc tcccgcccga caacaccggc
     8281 gcagcctctg cgagccggcc ggaaccgtcc gtggcccatg gcctgcaccg ctgctctttg
     8341 agaagtgaag atcgactcct ccggccgaaa gccaagaccg gttcgtgacg gtctgggcgg
     8401 cgctttcaga accccgcgcg gaagcgagcg ggcggaccgc aaagggccaa gatcgccagg
     8461 gacgctgtcc ctgcggtcgc ggctctgaaa aagccgcagg gtctcccggt ctccccgcca
     8521 caacccccgc ccgcctttgg gacaacgaag ataccttccg cgccaaacga acccaatgga
     8581 tgacctaacc tattgtataa taaagagata caagcgtctt ccagtctgag agtcaattcg
     8641 gcagcttctc acccaaggct gcgcagatgg cctcgagatc gtcggccacc tttcgcggcg
     8701 tgttggcaaa cctgcccttg ggtggaacgg gtggcggggg gggaatcgcc ttcatgtcga
     8761 tgccggtatg gtacttgacc gtggcatccg ggtccttcaa ctcgtgcccg gtaagaatgc
     8821 aaaccacctg ctcgtccggc gagatgatct gctcgcgcaa cagcatgtgc agaccggcca
     8881 cactggcggc gctggccggc tcgcagccga acccgtaccg gccgaccagg gccttatgct
     8941 ccagaatctc atcgtcggtt acctcccgca ccaggccgtt cattacttcc agggctcgca
     9001 gggccttggg cagattcacc ggccgcgcga tctcgatcgc cgaggcgacg gtgtgcggca
     9061 ggtagccggt cgaatcaaac tcggcgtaaa gattcttcac cttctcctgg tcatagcggc
     9121 cgccgttcca ccgcacgcct tccttctcat gcagccggta cagcgtattg gctcccgtcg
     9181 cgttgatgat cgccaaacgc ggaattcgct tgatcaaacc cagacggtgc agctccagaa
     9241 aagccttgcc gaaggcactg caattcccga ggttgccgcc gggaacgatg atccagtccg
     9301 gcacctccca gctccgggcc tccaacacgc gatacatgat cgccttctgc ccctcgagcc
     9361 ggaacggatt catcgagttc atcaggtaga tcccgagctt gtccgagatc tgtttgacgc
     9421 gccgcatgca ggcgtcaaag tcgccctcga tctgaagcgt cagagccccg taatccaggg
     9481 cttgcgagag cttgccgtac gcgatctttc ccgagccgat gaagacgatg ccctgcatcg
     9541 gcggatcggt cttgttggcg aacatcgcca gcgaggcgga agtgttgccg gtgctcgcgc
     9601 aggccacccg cgtccttccc agggcccggg ccagcgagaa agccgcggtc atgccgttgt
     9661 ccttgaagct gccggacgga ttgagcccct cgtattgcag aaacagacgc ccgggcttca
     9721 tgcccacctc ggccgccagc cggttgttct cctccagcag cgtccggccc tcgccgatgg
     9781 tgacgatctg ctcgggcttg acgaagggaa taagctctct gaacctccaa acacctgaaa
     9841 aatccagcct accctccgcg ttcaacccct tggtggccca gcggtgctcg aagaactcca
     9901 gcgacgtggg caccgccagc cggtcccaat cataggcgat gtcgaacagc gaaccgcacc
     9961 tgggacaact gaaaaccact tgatcgaccg agagggtctc acgacagtcg ggattgatgc
    10021 agatctggta agctttctca gccatcgcat gtcctgttcc cggcggcgcg ccgccggttg
    10081 cgctcagggt gccgcgccaa ctgactcctg aacactcttc cgggaagtcg gggcgcggcc
    10141 cagccgggtc gtatgatggg acaatataag ccggacgaat gccaagtcaa aacggcctca
    10201 cgccctccgc tgagcctctt catcccgctc tcgttgtcgc cgcgtggcct gggcaagaat
    10261 cttcttctct ttacgcgtca ggctggccag cccctggtca tgaaccttcg ccagaatccg
    10321 atccacctcg gcctgctcct cagcgagcat tcgctgtttc ttctcccagg cgccctctcc
    10381 tcccttgctt cgccggtcgc ccgagccggg aaggatcgtg acgcctcgac cgaatcccaa
    10441 acggatacga ggcatgactc cccatgcgta gaagaccacg atcgccgcca tggccccggc
    10501 ggcgtgacac aactctcccc cccggttgaa cgaagaactg gggctcagca cggtcaacac
    10561 cgcgattgcc aggacaatca gcccgaaaac gcgaatgggc atcggaaaga cgagcaggat
    10621 gatctggatg tgagggaaaa agatgattgc agccatgagc agcccgtaaa cggcgccgct
    10681 ggcgcccacc agcggaatgc caacgtgcgt ttcatccgcc caacccagac caaggtaagc
    10741 cagcccgccc agcaggccgc aggacaggta gaactggaga aagcggtgct ggccgaagct
    10801 ccgctcgaat tcactgccaa acacgtacaa gccgatcatg ttgaaaacga ggtgccacgt
    10861 gtcggcgtgc atgaacatgt aggtgagcgg ttgccagagg cgcaacgata agacgccatc
    10921 caggctcaaa ccaaaggtca aaacggagta gctctgactc cagttctgag acagactgtg
    10981 aaagaggaag acgacgacgt tgatcacgat gagccacatg acgatcgtgc tcggccgccg
    11041 caactggatg cgcagttcag gctgggagtc gtcagccgcg taaggtctgt cgcgccagct
    11101 catggcttgt cccccgaccg ataccgatgg tggtagacat gaagcgatgc gatggtcttg
    11161 ccatcaacga tgcgaccgtc ggttgtcgcg gccagagcat cattcagggg catgatctca
    11221 atgcggatct gctcggtggc ttccaatcgc tgtctcgtag gcgtcagtcc ggtgcccaca
    11281 aacacgtaca ttcgttcatc ggtgaagccc ggtgtggtgt agaactcgca cagccgctcc
    11341 agctgggcgg ctttgtatcc cgtttcctcc tccagttcgc gggccgcaca ttccatcggc
    11401 ggctcaggcg gctccagcgt tccggccggc agttcaagca gctcggcccc ggccgcgaac
    11461 cgatagttcc tgatcatcac caccgtctcg gaatcgagca gcggtacgat cgtgaccgcc
    11521 cccgggtgga cgaccagttc acgccgaacc attccctgcc ccggaatgtg gtattccagg
    11581 cgctccactc tgaacctgcg ggtctgaagc agcacttcgc gcatggaggc tttatcggca
    11641 acctgtaaac gacaattcga cgccttcgat cagacgtccc tcaggaacac ccggaacctc
    11701 aacggctcaa tcggattata actactgccg cccggcgtgc gaggaacggc gcgagtcgag
    11761 cgccaagccg ctcgcaggcc gctcgtgtgg tccggggctg tcttgtcagt ctcccgacgg
    11821 attcttatag tgaagcgaca gtgccaaggg agtccttacc actagaaccg tcggccggag
    11881 ccgaggccaa gcgcgtctgc atcatgggat cgaccggatc catcggcaga agcacgatgg
    11941 acgtcgtgcg ctctctcggt ccaccgtatc gcgtgacggg attgtgcgcc gctcgccgat
    12001 ggcgggagct ggccgctcag gccgccgagg cccggccctc ggccgtagcc ctcgctcaga
    12061 cagatcatgc caaggaactc aaaccgctgc tgaacgggtc cacagagctt cttgttggcc
    12121 cggaaggcct tgtggaactg gccgcgcgac ccgacagcga tttcgtcgtg gcagccatcg
    12181 tcggcgccgc cggcctccct tcgacaatcg cggccgtccg agccggcaag cgagtcggac
    12241 tggccaacaa agaagccctt gtggttgccg gatcgatcct gattcccttg gcccgcaagt
    12301 ccggtgcctg cctgttgcca atcgacagtg aacactcagc cattttccaa tccatgacgg
    12361 ccggccgtcc gtccgaggtg aagcgaattc tccttaccgc cagcggtggt cccttcagaa
    12421 cctggtccgg ccccgaaatc gcccaggcta ccgtcgaaga tgccctcagg cacccgacct
    12481 ggaaaatggg accgaaaatc actgtcgact cagccacaat gatgaacaag gcccttgaaa
    12541 tcattgaggc tcgctggctt tttgggcttc cggtcgagtc gattgaagtg ctggtccacc
    12601 ccgagtcgat catccattca atggtcgagt tctgtgacgg gtcggtgatc gcccaactgg
    12661 gaacgcccga catgcggacg ccgatccaat actcgctgac ctaccccaat cgcgtgccgg
    12721 ggtgtgcgac gcggttggac tttgctacga tccgcaggct gaacttcgag ccgccggatc
    12781 tggagcggtt tccggctctg agactggggt acgaggtcgc tcgagccggg gggaccgccg
    12841 gggcggtctt caatgcggcc aacgaggccg ccgtcgaagc cttcttgaac cgacgaatcc
    12901 cattcggacg catcgtacaa ttgattgagg aggtcctggg acggcatgcg gtaagcccca
    12961 gcccggatct ggacgaactc ttggaggccg acgcgtgggc tcggcgcgag gtgaatgaat
    13021 gcttgaagga ctgatcggac tatccgctgt ggggcaagcc gtcccattgc tcgggagcgt
    13081 aatgggcaca ttattcggct acgtgctgat catccttggt ttctcgttcc tgatcttcgt
    13141 acacgaactg ggacacttcc tggccgccaa gggctgcggg gtccgggtgg atcgtttcgc
    13201 cgtcggtttc ggcagggcct tgtgctctta tcggaaaggt ctcggtttcc gctggggatc
    13261 ctccgaggag caatacctgg agcgattgag ggaacacgtg gagcgcaagc ggcaagccga
    13321 gcttcagttc aaggagaaga cttcagtcac cgacgaagag ctggcacgcg ctgcccagga
    13381 attgaagctc ggtgaaaccg agtatcgctt caattggatg cccctcggcg gctacgtgaa
    13441 gatgcagggg caggaagact tcgaaatcga taaatccggc gagatcgagg ttaagaacga
    13501 tcctcgctcg ttcctgaaca agtcggtcgg acgccggatg atcatcgttt cctccggcgt
    13561 ggtcatgaat gccatattcg ccggaattct attcatgatc acttacatga tcggcaagcc
    13621 ggcaattccg ccgattgtcg gcggagtcat gccgggttgg cccgcggatc gcgccggcct
    13681 cctgcccggt gatcggatca tcgaagttaa cggcgagcgc atcaccgatt tcgacgtcct
    13741 tcggatggcc accattctgg ccgaccccaa caagccgatg tccatcgtct tcgagagaca
    13801 ggagcacaag gatggtccgc ccgtcaggaa taccgtcgag attcagccca acatggatga
    13861 cgtcttgggc gtgcttgtgc tcggaatccg ttccggcgga aacaacatta ttgccggcgt
    13921 ctggccggat cccaagcttg ctcccgagga gcagccgcaa atcggtgacg aggtcctgga
    13981 ggccgacgga cgcaaaatca cgaactctca ggatcttctg gccgtcctcc agcggcgaca
    14041 gggggattat gcccgacttg ttgttcgtcg gaggctggag gacggtacca acgtcacccg
    14101 cgaggtgaag gcaagagccc tgcgtcgcct gctgcctcgc ggcgaaggca aagagagcga
    14161 tcaggaattg ttgggtttca tgccgcgaac ctgcgtcgcg tcagttgagc cgaacaagcg
    14221 agccgacctc gcggggctca aggccggtga cgtgatcctg aaatggggcg agcaaccgga
    14281 cccgacggct tccgaagttc gtgacgcgat cgctgccaac cccgagcgag acatacgggt
    14341 gagggtgctc cggcacgaag gctcgcaacc accccagcac gtgtggctcg tggtgcggcc
    14401 cgctgccggc gccttgggac ggggcagacc tcacgtaggc atgaacatgg aagccgacgc
    14461 cgaggggccg ccgttcgtcg cgggtattgc actcgaggtc cgcgagggcg ttcctacggc
    14521 ggctgcgcca ttgaaggaga agatgccgcg aggttcgctg atcacgagca taaacggtga
    14581 gccggtcgca acctggggcg aggtggttga tcgattcttc aagttcgccg ggggcgaggt
    14641 tcaggtggca tggcgcacgc ccgaagggct ggagcagcgc ggcagcattc gcgtgccggc
    14701 gacgcttgga acaatcttcc ccttgcccgg cgatcacgtc attgtcagga tcgacgaccg
    14761 tcgcaccgcg gaggttaaga tccgggatca gtatactcct gtcccggtcg gtacctggtt
    14821 gggcgcgagt catgctctcg aaatgtgtat cggccggaag atcgagatcg agcaccgcgg
    14881 catgcagggt aacgccggcc atactgagct ggtcgaggtg accagggaaa tgctcgacac
    14941 gtggccgatg agaatcgcgt atgatcgcgt gactttggaa acgtatgcgc ttcgcgagca
    15001 cttccgcgaa cccaacccga tcaaggccat gatgatcggt gcccgccaga cgtactactt
    15061 cattgaaacg gtctacctgg tcattcagcg aatggttgtc gatcgtaccg tcgggtttga
    15121 cacggtggcc ggaccgatcg gcatcgtgaa ggccggaagc gacatcgccc aggaaaactt
    15181 cagcagcctg ctctatttcc tcgcgctgat ctcggccaac ctggcggtga tcaacttcct
    15241 tccgttgccc atcttcgacg gcggtgtcat ggtctttctg atcatcgaga agatcaaagg
    15301 aagcccgatc ccgctcaggg ttcaggtggt tacccagctc gtcggactgg ccctgatcgt
    15361 caccatcttc gtctacgtga tgctgaacga tattcagcgg attttcggct gagtctgaag
    15421 gcaggatcct gatcagccgc ccggctgcgg ggtctccccg gatttccacc atgtttccca
    15481 ttgctcgcga agcatcccgg ccggtcgcgt attcgccggg ccgctggccg ccctcggcga
    15541 ggttcctccg gcaatcatcg tgagggaacg cagagccgct tcggccaccg tgtgagcgcg
    15601 ggccacagtc accgactcgg cattgatccc tctggcggcg aactgggcgc gagcatcggc
    15661 cagggccttg gcggccgatc gctcggaata gagcatggat cccagcgagc gcgtgtcatc
    15721 cagcagggcg atcaacccgg gcacggccgt tcgagacccg atgatgccca gcgaatccgc
    15781 aacctgcccg agtagatccg gctcgctcgc ctcagagaga acatcgacga ggatcatctc
    15841 cgcctgctct cctgggatcc gctgcagcgc cacgatggca tggcgggccg cctttccgcc
    15901 gcgggcgacg atcttctcca gctcggggat gagcgccatc gccgtcccac gtcgaggatc
    15961 ggtcaacgaa atggcggctg cgtcagccac ctcgtcatcc ggatcgttca gcagatcgag
    16021 caggacgtgc tccacctcct gcccccgaca gttgtcgagc acggcaacgc ccagcctgcg
    16081 aacgcgtgga tcggtgtttc ggacaagacg tttggccgga ccgaacgcac tctcctcagt
    16141 gcttgccagg cacagcaggt aatagtgtat gtcctgcgga ctcgacgact gggcgagctt
    16201 ccgggcccac cagtaggcgc ggatctctgc gcggtaccac atggccaggc accacaaggc
    16261 gaccagcagg gcgatgattg cgatggtcgg tcgtcgtccc cgaccgggga gaggggcatt
    16321 gcgttgagac ggcaaggagg tgccggactg ttccacgtca accattggga ctgactttcc
    16381 tgctcaggcg gcctatgccg ttcgggatgc gtcgtttcaa cgtcctcatg tgactgtacc
    16441 tgtcggacgc cagacggtca acgcgtgcgg ctcttcatcg cctccgcgtc cactgctcat
    16501 cgcggtactt gaataccagt cgctcagccg tctccaactc gccgttgctg agcttcgcgg
    16561 gtttgccggc cggggcacgg gtgatctcct cgaccagccg cggcagccag gtgttgatga
    16621 cgaccggcgc atactccgcc acgctcgcgc agcactgttg ctgaaaccgg ttgtccagaa
    16681 tgagcgaacc atgttgaagg atcgctgtgc gcaaacgcct ttgcgcgctt cccatgatct
    16741 tacgcccgtc cgtgagcaga tcgaaacggt tgtggcgatc gaagcagaag aacggccctc
    16801 gatgtgacgc gccgcccgat gcggaaggtc ctttgatcac cgagactccc agattcgtca
    16861 ggaagcgggc gaaggcgtcg tgaacgacgt cgtacaggcc ggcggcaccg cacgcgggca
    16921 gcaacggatg atttgacggc aacacgagag aataggtcaa ttccagatca tgaaggatgg
    16981 cgccgccgcc cgtgagccgt cgaaccaccg gtaggcctcc ggccggcggc ggcaatgcgg
    17041 catagtccga gtaggactgg aaatagccaa gcgagacggt gggcggcgac catcgataga
    17101 aacgcaacgt aggcggggcg cctccttccg atacggacag gagaatcgct tcatcacgcg
    17161 ccatgttgac cgggccgtcg gccggcccgt cactgatcca tcgccagtca cccacaagag
    17221 ccataaagat ccacatactg tttatctgtg gctcgccttt tcaacatggc caagcgagcc
    17281 gctgccactg tagtggccaa ccgtccgata aacaaggcct tcccagtctg caaggaagac
    17341 gtccggcgtg atttcgtcgc atgcggacgc gcgcgaccgg gccggggcgc ggtgacgctc
    17401 caaagggtgg aaatcctcaa ggcagctttg tagactgagt tctgttcagg ttggcgagtc
    17461 ccatatgcag ggttggtgcg gtcagccagt acaggccgtg aaatggtgcc aaggtccgga
    17521 caccaatacg cggcaagtgg tgagcgaggc gaggggtcgc agacagtact gtcctggggg
    17581 caaggatcgc ccgctgccgc cgctacgggg gcaagtcccg attcggattt ctccgcgatt
    17641 ttgtgccgcg acattgcgtt aacggacgcg ccgcaaccgt tttttggggt tgcggctgat
    17701 aatagcggcc ggtagcgcat agagagctgt ggaagtgcgt atcggacgca agaactccgt
    17761 cgccacagtg gactcgctat ggatcggcgg gaataatcag caacttcggt gcgggtgagg
    17821 agacatcccc gtcagaaagt tgcgatcgct ggtgaaggac accggacgat cggggatgag
    17881 aggcggaggt tgaggcgatg accgctttgt ttgtcgcagt attggtaatg gcaccaggcg
    17941 ttggagagtg ggagcccgtg gtgatggacg tttcggcgta ttgtccctgt ccgctgtgct
    18001 gcggagccca tgccagcggg attaccgcca gtggcaagcc ggcggttggc aagctgatcg
    18061 cagctccatc gtcttacgcc tttggtacgg agatgcgggt tcccggttat gggacagcca
    18121 aggtgctcga ccgcggcgga gcgatcaaat cggcggggga atgggtacgc aacgtaaaga
    18181 tcggtgatga gaaagtggcg gacgtcaaac tgcaacacga tcggattgat ctgctgtttc
    18241 cgacgcatgc cgaggccctc aagtggggtc ggcaaaccct gacggtgtgg gtcaggcgtt
    18301 aacccagacg gcaggagaag gagcgttgcc ccgatccgac ggcaatacat agaatcggtt
    18361 tttcagttcc tttgtttccc catggtatgg cgcctcggcg ggcatgatgc caagtccgag
    18421 gcgttttgct gcgcctgacg acttgccccg aagtaaagcc gcgccccgcc ggactgtggc
    18481 tctcatttac tcgagtcctg ctcagaccgc cggcggagat cgggcttgcg gaccgcggtg
    18541 acttcatcga ggcgatgcac gggcatcgta agcggtgcct ggcgaagaac ctcgggctct
    18601 tcgcgggctt ctctcgcgat ctggatcaga acgtccgcga agtgatcaag cgttgcaagt
    18661 gactcgctct cggtcggctc gaccatcagg cagtgatgca cgggccagct catcgttggc
    18721 gggtgaaacg agtggtcgat gagacgcttg gcaatgtcga tctcggtgac gccttgctct
    18781 cgaaactccg ggaccatgat gaattcatgg gcatagggcg gatcgaaggg gaagcggtaa
    18841 tggtcgcgga ggcgggcggc aaggtagttg gcggagagga ccgctttctc gctgacctcg
    18901 cgcaacccct cggggccgag catgcggatg tatgcgtagg cacggatgag gatcccgatc
    18961 tgcccgtaaa accagcggac gcggccgatg gattgggggc ccggggctgc ccaatcgtag
    19021 cggccgtctt caccgcggac gacttgcggt ccgggaagga agccgcgcag ccgttcggcg
    19081 accgcgacgg gaccggaacc cggtccgcca caaccgtgcg gggtcccgaa gctcttgtga
    19141 agattgaagt gcatcacgtc aacgccgaaa tcgcccggac gtgcgatccc caggatcgcg
    19201 ttcatgttcg caccatcaag atagagcaga gcgcccttgg tgtgcagaat gtcggctatt
    19261 tcggcaatct ggggatcgaa aagcccggcg gtgttcgggt tggtgatcat gagagcggcc
    19321 gtccggtcgt ccaccttgcg gcgaagatcg tccagatcga cccggccgtc gcggcgggaa
    19381 gccaccgtca ctatttgccg tccgcagact gcgcaggatg ccggattggt gccgtgcgcc
    19441 gtatcgggag ccaacacctt cggtcttgct tccccgcggc tgcgatgcca ggcactgatg
    19501 attttcagac cggtcatctc gccgtgggcc cccgcgcaag gttgcagggt cacctccgcc
    19561 agaccggcaa tctccgccag aaatatgcgc aagtgataga gcagctccag gatgccctgt
    19621 acgtcctccg caggctggag cgggtgcagt tcggtgaagc cgggtaagga ggatacccgc
    19681 tcaccgactt tggggttgta cttcattgtg catgagccaa gcgggtaaaa gttgccgtca
    19741 acactgaact ggcgatgagc cagtcgcgtg tagtgacgca caagatcgag ttcgccgatc
    19801 tccggaaggg ggggcggctc atcggccaac aactccgggg gaatgacgga tgcggcgtcg
    19861 atggcaggtg tatcgccggc cggcagcgag acggcggaat tgccaggagc gctgagctcg
    19921 aagacgagtc tttccggcgg cggcagtgca gctcgtccaa gggcatcttt gttcctcgta
    19981 gctccgatcg gctccgcgct catgatttcg tctcttcctg ctcctgccgt gtcacagcgg
    20041 cttgccccac cacgccttgt agctttcagc ggtgtggagc tgtttgctct gcatatcgag
    20101 aatcaacaac gaaggaaaat ccttctgcaa atgcaccaga atcctgatca gatcggcggc
    20161 gttgcagttg ttttcgacgt aaatggccgc gtgtcgggga aagatccgcg cataactcat
    20221 tcctcgacct aaatagtcca cggcgtcctt ggggccgggc cggcatcctt ccagcttgag
    20281 cagtgcggcg cgcacgacct gcacgtcggc gaaaggcgca gctttcttga cgagtcgggc
    20341 cgcagactcg ggttcaaccg gaaacttcgt gccggccggc atcgtcagtt gaacgaattc
    20401 gacgtcgtaa cccatggagc gcggtcctcg tattcaacgc ttgcccagag cgccgacaag
    20461 atgatcgatt tccttcttgc tgcgtttctc ggtacaggcg accatgaaac agtccgccag
    20521 ctcaggatac catcgtccca acggtacacc ggccaggatg cccgccctgc ggcactgctc
    20581 gagcaccctg ggcacctcgc ggtcgctctg aacgacgaat tccttgaaaa acggcgaggc
    20641 gaaacgaagc ttgaaaccgt cgactgccgc gatggcctgg gccgcgtaat gagccttctc
    20701 aaggcagagc cgtgcgatgc gggtcaggcc gtgcggccca acggcgctca ggtaaatggc
    20761 tgcgcgaatg gccagcaggc cctggttcgt gcagatgttg ctggttgccc gctcccggcg
    20821 gatatgctgc tcgcgcgtct gcagtgtcag gcagtaggcg cgccggccgc tgagatcggt
    20881 ggttgctccg accaggcgac ccggcagctt gcgcatgaat tcgtcccggc aggccatgaa
    20941 gcccagcgtg gggccgccaa aggacatggg tatgcccagc ggttgtccgt cgccgaccac
    21001 aatgtccgcg ccgagttgcc ccggccgttt gagcaggccg caactaagcg gatccacgga
    21061 ggcaatgacg agtgacccgg ccgcccgggc aatctcggcc agccggtcga gtctttcaat
    21121 gcacccgaaa aagttggggg tttgaatgac caccgccgtt gtatcaccgt caaccacgct
    21181 gcgaaactca tcctcgctcg tacggccatc ttgcatgccg caggtggcga tttccatcgg
    21241 taagtcgcga agataggtct gcagagtctc gcggacgtgc ggatggacgg tcatcggcaa
    21301 gaccacgcgg cctcggccat tgattgaccg ggccatcagg actgcctcgc tcaccgcgct
    21361 ggctccttcg tagagcgatg agttggacac gtccatgccg gtgagctcgc agatgagcgt
    21421 ctggtattca tacagtgcct ggagagcacc ttgggcggcc tcgggctggt agggggtgta
    21481 ggcggtcaca aactcgcttc gacaggccaa cgcgtccaca accgtcggaa cgaagtgatc
    21541 gtagattccg gcccccagga agcagacttg ccgattgcag ccttgattgc ggtcggcaag
    21601 tcgctccaga tccgcaagca gttcaagctc ggtcagcgga acgggcagat ccaacgggcg
    21661 atcgaggcgc aaacggtcgg ggatgcctcc gaaaagctcg cccacgtggc cgatgccgat
    21721 ggttgccagc atgtgctgca cgtcacgttc tgtcagttgc gtgaatctca tcgtgtcggc
    21781 ttggcttcat gaggccgctg caagcaaccg gtcatacgcc gccgcatcca tcagcgcgtc
    21841 gagctcggaa aggttcgcgg cgcgaatctt gaccatccag ccctgtccgt atgggtcatt
    21901 gttcaccagc tcgggggcat caccaagtgc cttgttgacc tcgatgacct ccccgctgac
    21961 ggcggtatat aactcgctgg tcgctttgac ggactcgatc tcgccgcatg cctgacctgc
    22021 cgtcactcgg gtgccgatcg acggaagatc aacaaaagtg atgtcggcca actgatccgt
    22081 ggcgaactgc gtgatgccga tggtcacgac gtcgccttct gcaagacacc attcgtgtgt
    22141 ttttgtatat ttgcgatcgc tcggagtggg actcacctta gcctccttgt atttgccggc
    22201 ctcgtggctt tgtacgtcag gtgccggtct tttgcttgta gaatggcagt ctgacgatgg
    22261 tcgcatcggc ctgtttgcca ctcaggtcca cggtgagcgg gcggccctcc tgggcatgag
    22321 cggcatcgac gtaagccatc gcaatgctgc gttcaagggt gggggagaac gtgccgctgg
    22381 tgacttcgcc aacgggacgg ttctcactca gcaccgcgga atgcggccgt gcgatccggc
    22441 ggccggtcag ttcgaggccg accagcctgc gcgaggggcc ggcctgtgaa attcgccgca
    22501 gagcctccgc gccgatgaaa tccttgccga ggtcaacgca ccattccagt cccgcgctga
    22561 gtgagtcggt gtgttcgtgc aactcatgtc catacagcgg catgcccgcc tccagccgca
    22621 gcgtgtccct ggcgccaaga cccgcgggtt tgatggtgtt actcgcgacc tcatccccga
    22681 cgagatagct gccgacgagc tgggctacgt ttgcaggcag gatgatctcg aaaccgtcct
    22741 cgccggtgta gccgctgcgg aaaatcgcgt agggggtgaa catgtagttg ccgctgcaga
    22801 aatggtaacg cctcagatcc gccacggcga ttggcagcag ccggctgacc agctcgacgg
    22861 ccagcggccc ctggatggcg accatcatcg tttcttcggt tcgatccttg atatccagat
    22921 cgctcccgct cgcgtgccgg cggagccacg cgacgatgcg gtcgcgattc gaggcattgc
    22981 agaccaccag ccagtggtcc tcgaaacgcg acacgatgac gtcgtcgaga attccaccgt
    23041 cctcccggca gatatgactg tacaacgatt ggccgacgac ggccttaccg accctgcgcg
    23101 tgcaaatacg ttcgagaaga gactcggcat ccgggccggt caattcgatt cgtcccatgt
    23161 gcgagacgtc gaacacggac gcggcagatc gagtatgccg atgctcctcg atgatgctcg
    23221 tgaacaggag gggcatttcc catcccgcga agtcgaccat cttggccccg agccgagtgt
    23281 ggagatcgta aagaggtgtt cttcgcacga ttccttccgt gagaaagcct gtcccaagcg
    23341 atgccatgtt tacatcggtt gctgagcgta ctcttgccca acgaggctgt caaacgcggc
    23401 cggccagcgc gtcggcgaaa cggatccgct cggcaagcag ttcgcccgcc cggtcaaaga
    23461 tgctctccgg aaaccccaag gcggttttga cggcgatatc ttggtcgagc caggacttca
    23521 actggcgaat ggtcctgtat tgccgaccaa gctggggcca gaagacgatc gggaatggtg
    23581 ggatcgggaa atcactgcca tggacgagct tatggtggat ttccggcatc ctggcgagct
    23641 tcttgagcca gaccgccttg ttgatcatgg tcatcccggc cagatcggcg taaagagggg
    23701 cgtcggcgaa ttcacctgtc aatgcgtcca gcagtgtatg cagcgtcctg gcggaggtga
    23761 cgggccagag cggcgggctt gcgacgtgag cgatgataac ggtgggagga cactgttcct
    23821 gccgtagttg ccgaagagtg cgaagccacg gtgcgggatc cttgaaacgt ggatgcatgt
    23881 tgccgagcgc cgcctcacag ccgcaatgaa tcaaaaccgg aatcttcagc gaggcggcac
    23941 ggcgaagaaa atcgatggtt cgcggatcct gcccgtcgat gttctgactc agcggaagcc
    24001 acttgaccag gacggctccg gccgccgcga cctcctccag catatccatt gcggttttcc
    24061 ggcccacgcg gcgataggga tgaatcgaag cgccgaagag gattcgtcgg ggatggcgca
    24121 gccacaactg acgcgcgtag gtgttggaga catagagatc cgagccgtgc tgccacagat
    24181 gacggcgcga cccgagcggc cggccttcat cggtatggta ctggtcgaac gccaggagaa
    24241 cgacgcggtc gacgttctcc gcgccgagaa tgtgatctaa cagaatgtgc tccacccttg
    24301 catcgaattc ctgttccgag gtgcggccgc cgccgagccc gaaatgcagg cgggccagac
    24361 gcatgaacac gccatagtac atcacgtcgg agaagtagga gtcgcaggga gcatattggc
    24421 cgggggcctc gaatgaaaac cggccctcgt cgccacagag acgccgtcgc tggccgatgt
    24481 ggcaatgaac gtccactctc atgtagtcgc aggtttcctc tgctatcgtc tgcggccaat
    24541 ccggcggggc cggcgagagt cgcgcctgcg gacggtcttg cgctcgactc tggccgcttt
    24601 cctgccgcga tcaggctctc ctcgtttcgc aaccgaagga cgagcacggc ccgtgccggt
    24661 tttcagcgaa tcggccgtca gaacaaggtc caactcacgg gccgacagat caacaccggc
    24721 cagttggacg ttcaccgcgt cgccgatcgc gatccgtttg cggctgcgct ggccgacaac
    24781 gcagcccgag cggacgtcga tgtcccacca atcgtcggga aggtccgcga accgcaccag
    24841 accgtccacg cgatacttga gcaactgaac gtagacgccg acgttggcga cgccggtgac
    24901 cactccctgc tcgatatcgc cgacgcgttt ctccagaaac cgcagtatct tcaccagtct
    24961 gatttcgcgc tcggccgcct ccgcgttccg ttcagtgaag ctgcagtgcg cgcccaggac
    25021 ggccaattcc tcgctggacg gcgcccggga cttgccctgc ggcgagtcga gttggtgctc
    25081 gagatactgc tgtaataacc gatggaccac cagatccgga tatcggcgga ttggtgaggt
    25141 gaaatgggtg taatggcggc tggccagggc aaagtggccg atcatggccg gcgagtactc
    25201 ggcctgggcc atggatcgca gcacggcaag attgacagcg aatgcctctg gttgaccacg
    25261 aacgctatcg agcaatcgga tcatgtcgga tcgttcaagc cgtttgggaa ccggcttgcc
    25321 caggacgcgg aggaagccgg tgagcttgtt ttgactctcg gccggcggct cagggtgaat
    25381 ccgacgcagg tgcggcacgc cgaggcggtg gaacaactcg gcgaccgctt cgttggcctc
    25441 gaccatgaac atctcaataa tggtatgcgc gaaggcggta tcctccggga tgacgtcgat
    25501 gacctcgtca ccgtcgttca cgatcagttc taccccgggc agatcgagca cgatcattcc
    25561 tgccgccagc cgccgtttct gaatcaggcg ggccaggtgt tccatactct tgagcaaggc
    25621 cacgacctgg gggttcacgc cccgcgtttc gcccgccagg acgcgagtga cctcgcgata
    25681 cgtcagccgc ttgtgggatt gaatgaccgt gttggcaaag cgactcgcga tccgcctccc
    25741 cttcgtgtca tactcaatga agacgctctt ggcgagccgg ggttcaccct cctgcaggct
    25801 gcacaggccg ttactgagga cctctggcag catcggaatg acgtgccggg gaaaatagac
    25861 gctattgccg cgcagccggg cctccacgtc caacgggctg tccggcctga caaacgccga
    25921 cacatcggca atatgcacgc ccagttcata ccgccctcgc gccgatctcc gaatggagat
    25981 ggcgtcgtcg aagtccttgg cgtcatcggg gtcgatagtg accacgactt cccggcgcaa
    26041 gtcctcgcgg cttccaagct ccgtttcgag cgcgtacttg cgcacaacgt gccgcacatc
    26101 gtctgccact tcgggtggaa acgcgtgacg gagatggtat tgatggatga tgctcagcgt
    26161 gtcgacgcca ggatcggatc tgcgacccag gacctcgatg atgactccgc gtccggctcg
    26221 cgtggcggag gggaactcgg tcagctcgac gacgacttgg tcgccggtgc gggctcgcgt
    26281 cgagccgacg tcgccgacaa tcaccggagc gtgaagagcg tgtccgtcgg ggatcataag
    26341 ccattgctcc ccttgccgga tcagctcgcc gacgaatcga ctctggccgc gttcaataat
    26401 ccgcgtaacc tctccctcga cgagggccga atcgcctcgc ttgccccgac gggtgattct
    26461 ggcctcgacc gtatcgccgg tgatcgcccc gccggcggct ccagggggga tgaacaagtc
    26521 cccgtgatcg gtcgggttag ccggcaccac gaagccgaag ccccgcgggt tgccgcggaa
    26581 ggttccgatg atgaccccga cggccgacgg cagcatgacg cagttgcggc cgccgagtat
    26641 gacgcggccg gctttcatca gggacttgac cgccgcgcgg aactcgccgt attccttctc
    26701 ctcgatgccg atggattggg caagccggcg cgcgcgctgc ggccggtaat ccttgcgact
    26761 gacatgttgc agaatgcgat tagtgagatc gtttgttgtg ttaatgcgtg actccttcac
    26821 cggctcgcac gtcttggccg gcatcctgtt agcttacagg cagagagtca aagcttcaac
    26881 cgggtcgaag atgagggttg ggctgcggcg gacctcaagc cgcaagatcg aacgtgccgt
    26941 ccgggcgccg gaagcttagg gcgcgggatc tatgcggcct gcgcgcacgt ttccggatca
    27001 cggtgcgctt ggggcgcggt ccgcccggga tcacgtccaa ctgtcatagt cgtcggacag
    27061 cacccatcgc cggacgcgtc gaacgatgtc gcgatggagg gccgcaacct gtttctccgg
    27121 cagcgccagc acaacgccga tttccgcgaa ggtgagcctt tcgcagtact tcagcgtgat
    27181 gatcagccgc tccttggtag tgagccctcg aagaagaaaa cgtttcagct ttttccacgg
    27241 gggcggggtg tatgtgatgg cctcgaatgc ggcggcctgc agaccggccc gacgcgagga
    27301 tgcggccgct ctggacggaa tctcacgttt cctggcgggc ggtgatttga ggcctgcgta
    27361 ttgcgatgcc atgggtgacc tccctgccgc ttgccgcctc cctgaaggag cggcaagctg
    27421 actgccgata gcgtcgcgct ccggcgacgt catcgcacat ggaaatgacc tcgtgtatga
    27481 caagccctga agtgaccgac tcacgggcga cgtggcaatc agaagcgaac gagcaggcat
    27541 gcattgccgt gaagaatcgc ggagcgactt gttgaatgct gcagtcatgg cctcctccat
    27601 cagtcaacac atgccgcacg cacaacgatg gatgacaggg ttcaaagctt acctgtcctc
    27661 ttcacgaatc gagatactgg attatcggac agctttcgtc gaggcgttta ccccaaagtt
    27721 ggggctggcg ggcgcttcca cggatggggc ggccggttgc agccaccctt cagtggctgt
    27781 ccgcaagtcc ggttggaggc gcggacgttg gaacgtggtc gttgcaggcg gccggatcgg
    27841 aacgagagcc ccgcttctca gtattgccgt cgggggccgc ggcgtgccaa ggactccgaa
    27901 tatcgaccgg atcagcgctg gctcagtcgc gccgcgttgg agtctgctcg gcttccaagc
    27961 cggcggtgtc tcgaaacccg gcagctcatg gagcccgcaa taggccggcc aaggaaatgc
    28021 cgtgacgtcg gccgctcgga tggtctcgga ggcggcgaat tcgccgccat tctcagtctg
    28081 atgctcaggg tccatcagct ctgtatgaca ttgactccga cccgtgtttg ccgctcgcgg
    28141 ctctgcgggc ggttgacacg gtccgggtac ttcctggcga actctctggc cgcccaggcc
    28201 atctcttcca gcgtgacgat cttgtattcc ctgtcaccaa ggatttctgc cacccgcaca
    28261 taatcctcag ggcagaagcc gttctgcgcc gaggtgttgc ccttggcgtc tggaacggcg
    28321 aacacggtta tgaatgccgg cttgtgcagg atgtcggcgg cggccttctg gatcgcctcg
    28381 gccatgtctt ccgccgtctt ctcgctcggg aagtagtgta gcccgctgta ccgcagaatc
    28441 accggggtgc cggcccgtgt catcttcact tgtcccggcc cgcaaggcag aatgaacgcc
    28501 tcggtgtgcg ggctcagctc ggcatagcgc tcgtagaccg ggcgggagaa gtggatccag
    28561 cagtccatcg tctgcaaatc tgcccgctcc atgaacttgt cggattcacg gaagaattcg
    28621 tcgggttccg gcatccagtt cgggtaggta tagcccgcgc cgctgcagcc gcagaagaag
    28681 tagtccttgt cggtcatggt gctgtagtag tactcggcca tggccggggc gacgtcgagc
    28741 atccgcggtt gaaagcccca gttgatcggc acccggccgc gatggggatc gtgccaggcc
    28801 ccgccctgga agcacatgtg gatcttgagg gcgtcgccct cggtggtcat gaaggcgacg
    28861 tagtacttgt cttcttccag gcgcaactgc gacttgtcga cgtgcgattt ctgtcggaag
    28921 gtcttcatct cgaccggcac ctgggcgtgg aaactgaggt tgggggcctc ggtgcacatg
    28981 atgtagttgc tgttcagcga ggccatctgg caatagcggt cctcgggttc accccagccg
    29041 tagacgccgc agttcggccc ggcatagctg tgaatgcgcc agatccgctt gttgtcctcg
    29101 gcgtattcgc tcttggtgct gcaccggaag atgaatgccc ttcgggccac cgccaggtcg
    29161 agggtgtgga tcgacattcc cgtccacaga ttatccacgc tgtagatcat tcccggttga
    29221 caacggggca ggagattatc aatcgcgtag tcgagcggtt cgaattcgtc cttccatcgc
    29281 ccgacgaggt catacgcgat cgtcaggtcg ccgtaccggg cggtcagctt ctcggcgata
    29341 tcgggcttga cgggcagaag gtcttccaga ccggccagaa cgcatgccac atagagcgat
    29401 tgctcgacgc tcgggtcgtg gaccaccagc cccttgaagg cttcgcgaaa gcggtcgatg
    29461 acctcaaaca cgtcggcgag cgtctcgaac tcgatgccct tggccctggc gtagtaatcg
    29521 cgccaggtct ggtcggccgg cttccagttc cagaactggg tgtcgtacat cagccgcggg
    29581 ccggtgcgat tgaccaggcc ctgcagggcg tacatcatca cttggttctt ccagccgttg
    29641 tcctccagag ccagatgccc cttggcccac tcgggttggc cttccttata cgacggcacc
    29701 cgcccgccgg tgatgttcgg gtcaatgaga gaatccaggt tgaaccagac ggccttttcg
    29761 ctcacggcag tgaatccttc cgctgaaata gcaattgtcg atcccgctcc gattcctgtt
    29821 cgtcgctgtg gtctccccga cggggctcat gttactgtgc gcatcatccg ctgcaaggtt
    29881 tccggggctt gcccttgagc ttggctgctg ctatgatgcc tgcggcgatg gagtccgccc
    29941 ctaaccggtc gcgcagacct gatgactcct acccggatcg acctgggtgg ggtccgcgcg
    30001 acgtagaccc tgtagataga ccctgcccgg ccgatcgcct gagcggggtt ttttgctgga
    30061 tgcagggctt gcgcgtcgca tggattggac atggtcaagc tggtgttggt ctttcttggc
    30121 ggtgggctgg gatcgttgtg tcggtacggg ttggccggct gggggcagcg gctggtcaac
    30181 ggatcgtttc cagtggggac tctcctcgtc aacgtcctgg ggtgttttgt gattggattc
    30241 ctcaactacc tgttcaacgg cggcgcctat ctcatccggc ctgagtatcg tgtagctctg
    30301 accatcggcg tgctcggtgg tttcacgacg ttctcgacat ttggctggga aacattcgcg
    30361 atggccaacg acggccaggg actccgagcc gtgatgaatc tgctgctcag cgtcacgctc
    30421 ggattctcgt ccgtgctggt cggctatcgg cttgccgaga agtggtttgg agccgggtga
    30481 tggccgcaat ggttcagtca ccgctggtgg aagccacgac gcagagtgat gcccggcgca
    30541 ggaggtagtt ctacatgacg gacgtatggt tcaccgcggc cttgtgggtg ggactggcgc
    30601 ttgtggccac cttgctttcc atctggctgc gggtggcgac ggccctctca gagattgtgg
    30661 ttggcacggt tgctcaactg atcatcggcg ccgtcgccgg agttgccgcc ctgggggccc
    30721 aggagccttg gatcaagttc ctttcgggga cgggggccat cgtcctgacg ttcctcgccg
    30781 gcgcggagtt ggacccgacg gtgtttcgcg ccaggtggaa ggaggcaacc gtcatcgggc
    30841 tcattgcttt cttcacgccg tttctcggct gcgcggcggt ggcttactat gtgttgcact
    30901 gggattccaa ggcgagctgg ctggccggcg tggccctgtc cactacctcc gtcgccgtcg
    30961 tttatgcggt catgctcgaa ctcggcttca acaaaaccga tttcggtaag gctgtgctgg
    31021 ccgcctgctt catcaacgat cttggcacgg ttgtggctct cgggctcatc ttttcgccgt
    31081 ttacgatccg tacgggcgtg tttgtcatag caagtgtcgc cgtcttcgcg atcattcctt
    31141 ttttgacgcc atggttcttc aggaggtacg gcaaccggcc ctcggaactg gaagcgaagt
    31201 acctgctgct gctcctgttc ggtatgggag cattggctgc atgggccgat agcgaggccg
    31261 tgctgcccgc ctacatgatc ggaatggtgt tggcgggcac ggtgggcaag gatcatgttc
    31321 tggtgcgtcg gctgcgcaca ctgacgttcg gcctgctcac gccgttctac ttcattcgag
    31381 cgggatcgtt cgtgtccgtt ccggccctga tcgccgcgcc ggtgactttc atgatcttgt
    31441 tgggtgctaa ggtggtcagc aagtgcctgg gcgtataccc tgccaccaag gccttccggt
    31501 acgagtaccg ccaggccgca tacaccacct tgctgatgtc gaccggtttg accttcggga
    31561 cgatctcggc gttgttcggt ctgacgcata acgtcatcac ccagagtcaa tactcgtacc
    31621 tggtcgcggc cgtcgtcgcc agcgccgtca tccccaccct gatcgccaat gccttcttca
    31681 tcccatatga cctgttgccg aaacgcacca gagctacaat gcccgacata cggccggccg
    31741 gcagccgcga gatgacagca accgccgtac tggacggcga atcagttctg cctgaaacgt
    31801 gagagggcaa accatgaaga ccattcttgc agctaatgac ggatctgagt cagccaaacg
    31861 ggcgttccag tttgcgctcc aactggccaa ggcattcggc tccagcgtgg ttgttgtctc
    31921 ggtggctcag ccatccgaac cccccacgtc ggtcgagacg cccgccttgc tggacgccgc
    31981 gacggagcat ttcgaaaaag actttgttgc ttttcgtgag gccgcccaag cggcgggggt
    32041 gccgctggaa accgacgttg tcgtcggtca tcctgccgat cagatcgttc atcaggcggt
    32101 ccaacggcag gccgacatga tcgtcatggg ccatcgaggc aagagcaggt tccagcgctg
    32161 gctgctcgga tcggtctcca agcgggttct cagttatgcg ccctgtaccg tgaccgtggt
    32221 gaggtaaacg atgagactgg aaggcgaagc gcagttgctc cgcgtgttca tcggtgaggg
    32281 cgacaagtgg cacggtgtgc cgctgtatga ggccatcgtg atgaaggccc gcgaactcca
    32341 cttggccggc gccacggtgc ttcgtggtcc catgggtttc ggtgcgcaca gccgtttgca
    32401 cacagtgaag gtccttcggt tgtcccagga cctgccgatg gtgatcgaga tcgtggatac
    32461 gcaggagaag atcagcaccc tcctcccgca cattgaccag atggtcacag agggccttgt
    32521 caccctggag aaggtccacg tgatcaagta cctcgcaaac ggtggggatg cgagaaagga
    32581 atagtccgtc ggtcgccgca tgagggtcac ccgtgtcgtc ggacaagagc tccgaacgtc
    32641 gagcatgcag ccgcgatcgg gaattcgttc gcgagcaact cctgcggccc ggccgatatt
    32701 tcggctgcga gcacgacgcc atgggtgttt gctttcttgg ccgggaggca ttcagtccgg
    32761 cggaatccga gtttcggcgg gcggtgtggc tgaaccctta cgatgcgacg ttcaggacgc
    32821 attgggcgat c
//
LOCUS       DAOTPW010000025        32299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1462_length_32299_cov_5.866797, whole genome shotgun sequence.
ACCESSION   DAOTPW010000025 DAOTPW010000000
VERSION     DAOTPW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 32299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32299
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1462_length_32299_cov_5.866797"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="P5316_03785"
     CDS             complement(<1..763)
                     /locus_tag="P5316_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012144695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRR84108.1"
                     /translation="MSIAKTLAGAAVAAILAVTTTVSAAPWGLPELSAKTKACIECHR
                     TENTPIYQQWGSSKHYRANVGCYECHAAQEGESDAFKHEGELIATIVSPKDCARCHEK
                     ETKEFAESHHSKGGRILGSLDNTLAEIVEGNNAMKTPAFPDGVSAAVVNGCWQCHGAE
                     IKVLPGGKLDAATWPNTGIGRINPDGSEGSCAACHSRHSFSAWQARHPDACGKCHMGP
                     DHPQKEIYEESKHGIAFKAFREKLNMDSPKWILGED"
     gene            complement(781..2031)
                     /gene="nrfD"
                     /locus_tag="P5316_03790"
     CDS             complement(781..2031)
                     /gene="nrfD"
                     /locus_tag="P5316_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRR84109.1"
                     /translation="MRRYFTFLWQCLRMSFVGDWRYYAWMSVLTVFALLGLHAWCRQL
                     VGGLATTGMTDQVSWGVYIANFTYLVGLAAAAAMLVIPVYIYRNMHLHDVVIFGELLA
                     VAVIIMCLLFVTVDLGRPDRSLHLIQRFNFPGSMLTWDVIALNGYLLLNLHICGYLIY
                     CAYCERKPSRAFYVPFVFIAIVWAVSIHTVTAFLYVGLGGRPFWNSAIIAPRFLASAF
                     AAGPAFIILTLQIVRRYTSYRVGDEALLTLRRIVQVAALINLFLLGCELFTEFYSDSA
                     HTASSRYLYLGLHGRNALVPWIWTAIAFNTVATLLLVLPSSRGLKVLNVACVLAIVGI
                     WIEKGMGLIVPAFIPTPLGEIVEYVPTINEVLVSLGIWAFGLLLYTVFVRMSVPVLSG
                     ELTYRRLYGPSRQDNNRSQAETAA"
     gene            complement(2028..3047)
                     /locus_tag="P5316_03795"
     CDS             complement(2028..3047)
                     /locus_tag="P5316_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR84110.1"
                     /translation="MNGPNDRQDEPQAAAPGSPGMTRRGFIRKSAVAAAGAAAVAAAL
                     SPLRNISPDHIPTLEQLLQKHYKEMTPEEMKAVLERISREVEKRYAVRPQVADIKPMT
                     GVEFVYALNISRCIGCRRCVHACVKENNQSRSPEIQYIRVLKMEKGSIDVETSDHHYD
                     PPTVPEHESYYMPVQCHQCANPPCVKVCPVEATWQEPDGITVIDYDWCIGCRYCEAAC
                     PYWARRFNFAEPGLTAEELNPNMAYLSNRPRAVGVMEKCTYCLHRTRVGRMPACVEAC
                     PTGSRKFGNVLDPKSEIAYILRHKRVFVLKQEVGTLPRFFYYFDERGSRYHEPLDPAV
                     SGGDA"
     gene            complement(3031..3615)
                     /locus_tag="P5316_03800"
     CDS             complement(3031..3615)
                     /locus_tag="P5316_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84111.1"
                     /translation="MPIPPKTDVPVWATDTTPVRHPTLKPETVIAGMTFRCSECHDLF
                     PSPPETDRALTQHRHIVLDHGINARCFNCHNRQDRNAFADDRGDPIPFDQPPVLCAKC
                     HGPVYRDWTHDVHGRTDGYWNDSLGSRRRLKCVECHDPHAPAFQAMRPARGPNTLRMG
                     DQTHHPAGTEKTKNPLLIYRQTPSGRDVTHERPE"
     gene            complement(4356..4808)
                     /gene="tnpA"
                     /locus_tag="P5316_03805"
     CDS             complement(4356..4808)
                     /gene="tnpA"
                     /locus_tag="P5316_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRR84112.1"
                     /translation="MGQTLTCLHYHLVFSTKNRLPQIAADFRERLYDYMGGIIKGEHG
                     HLFAAGGTEDHVHLLASFPAHPSVADVLRVIKTNSSKWIHETFAEHRAFAWQSGYGAF
                     SVSRSNDDSVRRYISTQQKHHQQMTFQEEFLEFLRRHEIPYDERYIWE"
     gene            5050..6264
                     /locus_tag="P5316_03810"
     CDS             5050..6264
                     /locus_tag="P5316_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR84113.1"
                     /translation="MRTVSIVGCASYEASVVDAAVRQSLDYLGGMSRFVRPGMRVLLK
                     PNLLAPRSPSEATTTHPAVVAAVARLVQEAGGIVTVADSPGGPFVASYLKTVYAKTGM
                     EQVARETGLALNHDLAARQVANPSGKLLKHVDIIRAVAEADLVINLPKLKTHGQMVYT
                     GAVKNLFGAIPGTGKIDFHLRMADYQAFADALIDICLAAKPGLNLMDAVVGMEGAGPS
                     AGDPRHLGLILAGEDAFALDYVALQVLGANPIRIPIMKAGTERGLCPASLDDVTIMGR
                     SVEDVRVKRFDMPALSQMLTITWSQGRLLGRLAQWTKPRPVFDHVACTGCSLCARSCP
                     AHVIAMETGKPCVDLKGCIRCFCCQELCPAKAITIRRLRPSVAAILRIVYFTLSMISS
                     RLHRRKRQPRQD"
     gene            complement(6316..7266)
                     /locus_tag="P5316_03815"
     CDS             complement(6316..7266)
                     /locus_tag="P5316_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84114.1"
                     /translation="MNRTSGLAMMMAALIMVCGQPDPACGCCDACDPTGHLGVAMVVE
                     DNKIHTGVYAYDPSNPVTVDIGVHVWGGGHFQENPLDPFWTDDPCYGSTTDSGLPPGS
                     QVGFNILSDLLYWDGNGSVSFGPVPAGERLRIKYGFMTRHAGGGTGFVEGFNFQTVPS
                     DGSFHRHLSYFLLGPDGNSIPADQDGVQAADGVYLLEIELTNTAGIASSDPIWLVFRN
                     FSPSDPLAEVKHCMALLHVAHDIAHDRPRADLDFDRDVDVDDLERFEACATGPGIPYV
                     RECCRAADLDRDGDVDQADFGRLQRCFSGPEAHPDPRCAD"
     gene            complement(7371..8096)
                     /locus_tag="P5316_03820"
     CDS             complement(7371..8096)
                     /locus_tag="P5316_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84115.1"
                     /translation="MKNVFISSVLIVVLLGNVTLAAHVDVRAYVQNGAIAVGSSELVG
                     STVVPLSDRERVFGAEFGEDDPAQPFMTEDPGFLSEDGAFPGGSGHWLGFNALSGLAF
                     WNGSGFAGVPAFESLQITVGSQSVNVANDPAGGFNFAQIGAGGGLHQHMTFELLGSDG
                     NPIPGDGVEPSPGIYLLELELTTTMSDVVSSAPIWIVFNNGDSEANHEAAAAWVENNL
                     VPEPLSALLLVAGALPCRRRRGR"
     gene            complement(8156..8971)
                     /locus_tag="P5316_03825"
     CDS             complement(8156..8971)
                     /locus_tag="P5316_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR84116.1"
                     /translation="MRACSKTRLRGSAFTLIEVLVVVAIIALLVAVLLPSLSRARQAA
                     RRTMCLNNIKCLETAHWLYMTSNDGFLIRVGLMHGVADDKPQVAWFNTLQRYYKDRLV
                     ARSPADDSPHWPVELGGKGIPVDGKRGYPFRRSSYGINNFLDIEKNPDLTGKGVTWPK
                     IEKIRNPSSIVHFVYMVEECDPDNPYYRSGECFAASDHPHVEEWADVSHSPTKAATQL
                     EIQAHGGPKWSAHSLSNYGFLDGHAESLQFQQVYKDMEHNKFDPGLFINYHRR"
     gene            9815..10960
                     /locus_tag="P5316_03830"
     CDS             9815..10960
                     /locus_tag="P5316_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84117.1"
                     /translation="MNSRIARWLATAALFCAWTTATTAQEPDWASMSYTLHQDYQAVD
                     ANGQGTFPTTSPVKIKGVILNRPSDMLNPTPGFDPYMGGQWQQFVQFVDEGDFGGTAL
                     YMGQNIGKNVGNHPAGSYSDAEWLAELHRLNHDPITGRAFRSGDLVEVRARAPGLFFR
                     GKTNVNEQHQKTPLADFDVVLLQANYVSPAPQTITLADVKDTADQFIFDQSRATGAEH
                     YQGSFVRFENVQFVAGTWGCNQSMTIADDTGRTLPVLLGFGGGFTQYPAPTGTFDIVG
                     IFDQEDKVSTDGWKGGYRLWVMDYDGSHFVLYRYVKPDFDRDGDVDETDLDHFETCAS
                     APGIRQTDPSCLNADFDEDGDVDQSDFGVLQRCFSGSLELPDSACDG"
     gene            10971..11717
                     /locus_tag="P5316_03835"
     CDS             10971..11717
                     /locus_tag="P5316_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR84118.1"
                     /translation="MAHDRRQVRSTRPGGGRCARNGVVEAFSLVEVLVVVAILALLAA
                     MLLPALARAKLRSRVVLVHSDLRQVTIALDAYALEHKDRLPPTRQSCGTSILYQLPFE
                     LATKKYLPKSPDAIKRSYLEDAFNPGQTYRYRAPGPVWQNGVLMDFPDSTWRPRAQIW
                     VPDDVPNCESPNGRYYADRNNEPKSPVTYAVWSVGPDPHSPKFPRWDDGTIDEAKLPL
                     PKSFWLLNDGRPTGLITHFRTRKGVTHMSP"
     gene            11842..12381
                     /locus_tag="P5316_03840"
     CDS             11842..12381
                     /locus_tag="P5316_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HRR84119.1"
                     /translation="MDFYTNRQAEDPDWLARAALFHGHLGPWLTVGAMIGQDAIRRLE
                     TPGQWKIHVTCWMPADKHRTPFTCILDGLQAGCGATMGKRNLWLRDSAEVLSNRWPVV
                     HVVRLPDGGRPAEGLSYTATCALHGWMEHLKPERLEEDSRALAREDVTKMFRITSMGE
                     REFAWVGCSSAGQVRATTP"
     gene            12378..13241
                     /locus_tag="P5316_03845"
     CDS             12378..13241
                     /locus_tag="P5316_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRR84120.1"
                     /translation="MTQRTAIQAVGLLHVFGLAVTLAGCNRSSPPQSPPAPNPLAHVA
                     AGNSYLEAALIDLMGDASRPLRLAEPGTCPGHFDVRPSQVSALRTCRMLVRFDFQRAL
                     DDKLSPLARDGLRIVPIRVSGGLCIPSSYLSACRQVAEPLVAANMLSAEASRHRIAEI
                     EQRMSEREEWCRRQMREAGLEGKPVVCSPHQEAFCRWLGLNVVSTFAGADSASIGDIE
                     TAIREGENARVGIVIANLPEGRRLADALAQRLGAKVVVFGNFPQDDAGTISFDALIAT
                     NVTSLVKAATP"
     gene            13238..14041
                     /locus_tag="P5316_03850"
     CDS             13238..14041
                     /locus_tag="P5316_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR84121.1"
                     /translation="MNDTAITIRDLIVRAGRRNILAIENLQLPKGQFVGILGPNGAGK
                     STFLRCLLGLQRHAGGRVTVLGEPLAELGMRGLGLLRRRIGYVPQLLSSPGEIPLTVR
                     EVIAIGRTGIAGLIRPLRKKDWRIVDQWIERLGLASLTLRGYGEISGGEQRKVLIARA
                     MVQQPDLLLLDEPTANLDLGWRERIVTVVKDLYASSGMTVILVSHDLEVLPPACGRVI
                     VLERGRLVADGSPDDVLTDQRLETLYGPGLTVLKRGGRLLAVPAEVFNA"
     gene            14034..14855
                     /locus_tag="P5316_03855"
     CDS             14034..14855
                     /locus_tag="P5316_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRR84122.1"
                     /translation="MLDFVFWAPVLVSGFLAGSSSGLLSVLIVGARIPFLGICVAHAA
                     LAGAVFGALAGLEGQLLLLPAVAASIVTAVVLGLADPRKVRMDTNVVLGLLFSLTMGL
                     AFLGFGLFAVFGRSDDDVRGLLWGSLAYCRWADVVLMTVVSAALLAFVLLFGKEMRAI
                     VFSRQDALAAGIHATAVWTGFLVLAAVLITVNFQTVGGLMIYSLITNPAAAAFQLVRG
                     MRKASVVAALLGGVSGLGGFLIAAATDLPAGAVIVILSSLLVAGAILLRRIREAR"
     gene            15112..16842
                     /locus_tag="P5316_03860"
     CDS             15112..16842
                     /locus_tag="P5316_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84123.1"
                     /translation="MSPSHESSGIAGRLDKLYEFERAPVTPDRLHDGRYFAGLFAGEH
                     VAATEFVIGALFVQWGAGARNLVLGLLLGNLLAVLSWAFICAPIAVRTRLTLYWYARR
                     IIGPGLTAVYNFISGILYCCLAAAMIGVSASAVILGLSRLGLHVRHPALTDVCPNSVG
                     WLVVVLIVGSVTTILAIAGFKRLSQFAGVCSPWMFLIFIAGAIATLPRLGDVRSLDDF
                     WRIAETRIWLETPKAGTERLGFWHITLFAWFCNLAMHVGLSDMAIFRYARRWTYGFYS
                     AFGMYLGHYLAWICAGIMGAAVSEGLNPGEMAHLAAGLPGVLAVLLAGWTTANPTIYR
                     AGLALQIVTPNWPRWKVTLATGAITTLVGLFPAIFMKLLNFVAIYGLVLMPIGAVIFA
                     EHWLFPRTGLRQCWVERRGLSVNLPALIAWLTVLVLCFPIEDFTAGRLRSPMDLMGVG
                     LFFRWLPAWFLTVSVYLLLCRLTPGARRQPDEQAEISLEARSMITGPVAVRTSAPLPP
                     IAWISGALAVMALAACLILPMLVFIGGSDPSAYERNMGSYKTGLMVATVAYFIGGIIW
                     MVQREKARLT"
     gene            17149..17628
                     /locus_tag="P5316_03865"
     CDS             17149..17628
                     /locus_tag="P5316_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRR84124.1"
                     /translation="MKPTVYIETTIPSYYCDDRPELMGDIARTQEWWDKERGDYECFI
                     SPVVIEELSAGNYATRSKCLRLIDSIPLLEVNREVAQIAAIYQEQKLMPSPPVRDALH
                     LSLAAYYRLDYLLTWNCRHLANANKIPRLQKLNRELNLSMPLLVTPAMLQRWEVGDE"
     gene            17621..17812
                     /locus_tag="P5316_03870"
     CDS             17621..17812
                     /locus_tag="P5316_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84125.1"
                     /translation="MSDSPIVQEVRRRAMEISARFDHDLDRYVEHLREYQEQFRDRLV
                     SQITVVQSPRHGQEVGKPK"
     gene            17926..18771
                     /locus_tag="P5316_03875"
     CDS             17926..18771
                     /locus_tag="P5316_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRR84126.1"
                     /translation="MTSYLLLATLVLLSQAALPQSRPSSQQPGLRVVTWNVHGCAAGV
                     DGIVDELRTIDADVICLQEAEVGTAHAEGADQAALIAERLGMKHYSAGSRFAEGGGEQ
                     RMAILARGELEQTGPLDAGTGRIYGVAAVTRWNGRPLRVVSIHLTSTYRVDLRHAAET
                     SRARFKEASDLAGRPKKWTEDVVVAGDFNAIPGSPEHVVLAGGLKHVATTQPTFPSDR
                     PRLPIDHVYLSDGLRADLRVVRDSLASDHRAVIVELTAVRGPYSQPADTAPVSQGTGS
                     SIHRR"
     gene            complement(18738..19028)
                     /locus_tag="P5316_03880"
     CDS             complement(18738..19028)
                     /locus_tag="P5316_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84127.1"
                     /translation="MAVMSDEAAMVCHRCGAELHPGRGDFYVVRIDAFADPSPPVITE
                     NDLKRDLRAEIDRLVESLDGLSEQEAMDQVHRRLVIHLCVPCYRRWIEDPVP"
     gene            19307..20569
                     /locus_tag="P5316_03885"
     CDS             19307..20569
                     /locus_tag="P5316_03885"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HRR84128.1"
                     /translation="MSKTKSVEKAYQLAKERYAEIGVNTDRAIAALKKIPISLHCWQG
                     DDVGGFESTAGLTGGGIQATGNYPGKARTADELRADLDVVYSMLPGKHRLNLHAIYLE
                     AGKKVDRDEIRPEHFANWVAWAKGKLHGLDFNPTYFSHPKAADGFTLSHPDADIRKFW
                     IRHGIACRKIGESFGKQLGTPCVTNVWIPDGYKDITVDKKAPRERLVKSLDAIFAEKI
                     NPKYHLDAVEGKLFGIGAESYTVGSHEFYLAYAVSRGVLLTLDAGHFHPTEVISAKLS
                     AVLCFTKELLLHVSRPVRWDSDHVVILDDELFAIAQEIVRNDYANRIHIGLDYFDASI
                     NRVAAWVIGARNMIKALLAALLEPTDRLRKYERDGDFTSRLALLEEHKTLPLGAVWDF
                     YCLQAGVPVGAEWLAKVKDYETKVLSKR"
     gene            20683..21858
                     /locus_tag="P5316_03890"
     CDS             20683..21858
                     /locus_tag="P5316_03890"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HRR84129.1"
                     /translation="METNPALGVMIFALGGLAGAVFYLPFKKVRNWAWESYWMIYAIA
                     GLIVVPWVLALSTSPNVVAVLRQSPGTVLASCFGYGALWGLGGFTWGLMIRYLGVGLG
                     LAIGCGLCSATGTLIPPIIHGDPQKLYDSTSAVVSLIGVVVSLLGIILVGMAGMSKES
                     ELPEEEKKKAVAEYNFRKGIIIAIFSGLASSGMSFGLQGGQITEYKARFGSQTQLVKS
                     EDSVVPPADLVGAAGIFYDKDAKTWVAPRAPEGESTTSQTWRGMPILVVVLAGGFAVN
                     FLYCLYLNLKNKTLGDYAKKDTPLVGNLFFAGLAGAIWCCQFICFKTGEPAMGPRAYI
                     GWAVLMASAILFSTLLGILLGEWRGTSKRTRTCLAAGLALLIVSSVIAGISGYLKTA"
     gene            complement(21887..22717)
                     /locus_tag="P5316_03895"
     CDS             complement(21887..22717)
                     /locus_tag="P5316_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRR84130.1"
                     /translation="MPASTLASGFAAPDEWRVTVATLNVYFENRSPEPAVKAIRESKA
                     DAVFLQETTSKIEQLLRKELKDLYPYTVFKGGGGRIPTDRFGVLARSPIEKTRFLPPR
                     SGLFGTLVAQTVMDGRRLQLVNVHLKPLQLQPNARPAQHLQAFKALDKAHTAELGFIL
                     KSIDPKLPTIIAGDFNSIPPSSGPRLLAEKGYVDAFKLPSTNPAENEEGSGTPTWQID
                     TELGPIRLRIDYIFCSKDLTAVTGKVIPDTGSDHRLVVAEIGLPKGDASTQPATTAGP
                     "
     gene            complement(22910..24724)
                     /gene="aspS"
                     /locus_tag="P5316_03900"
     CDS             complement(22910..24724)
                     /gene="aspS"
                     /locus_tag="P5316_03900"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRR84131.1"
                     /translation="MAIAYNKRTCDCGTLRASDVGKSVILAGWVNNYRDHGDNLVFID
                     LRDRGGLTQCRFNVDTDPDATKLARSLRKEDVIVVRGEVISRGQNVNPKLPTGEIEVS
                     VHEVQVLNKSLTPPFEVADHVEANEDLRLKYRFLDLRRGPMQKGIITRHKVLQAFRQF
                     YYHEGFLDIETPILTKSTPEGARDYLVPSRVWPGQFYALPQSPQLFKQLLMISGFDRY
                     MQIARCFRDEDLRADRQPEFTQVDLEMAFVDVDNVLDVVERSLSYVWKQVIGVDVPTP
                     FRRMRYDDAMRDYGSDKPDLRYDLKLIDVSEVARKTEFKVFTDAIARGGVVKTICLPG
                     GAEMTRKETDGLTAEIQGIGAGGLPLCKVVAGASGKLELQTGAAKFFPGELGDEVIRA
                     TGAGNGDMIFFAAGRFADVCKYLNWLRTTLAERRKMIPKDRWEFLWIVDMPAFEWSED
                     ERRWVFMHHPFTMPHDEDLPILESNPGAVRAKCYDIVLNGVELGSGSIRIHDRGVQSR
                     IFRMLGLSDEEAMTKFTHLLTALQYGAPPHGGLALGLDRIAMLLAGAASIRDVTAFPK
                     TQKAFCPLTECPSEVDPKQLDELGIDLNAATKAKKKGK"
     gene            25424..25900
                     /gene="rpsG"
                     /locus_tag="P5316_03905"
     CDS             25424..25900
                     /gene="rpsG"
                     /locus_tag="P5316_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRR84132.1"
                     /translation="MAFKKWTASARQLRPDPRFNSKLVSKFINCLMYDGKKAVATRVF
                     YDAMDIVAKRVKDHSPMEVFETAINNVKPSIEVRSKRVGGSNYQVPRPVNRKRQQSLA
                     IRWLLEATRGRKGKPTCEGLAAELIDAFKGEGTAITTRENVHRMAEANKAFSHFAW"
     gene            26012..28837
                     /locus_tag="P5316_03910"
     CDS             26012..28837
                     /locus_tag="P5316_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRR84133.1"
                     /translation="MIDSVQERLSRSTDCEIRFDHLARLLYATDASIYQIMPLGVAFP
                     KSAAQAASLMRAAADAGVSVIPRGAGTGLAGATVGEGLIIDFSRYNRRITEFDRDKCV
                     VRVQPGVVLDQLNAFLKPHGLWFGPDVATSARATLGGMIANNSSGAHAPVYGTTDDHV
                     LGLEIVLADGTLATIGVAGDGLPEHREAVDQIINRCARQIEERFPPVLCKRWPGYGLD
                     RYLRDKGNLTHLISASEGTLALITSAVLKVVPIPGRKRMGLVFFDSVEEALAATVELV
                     DLKPAAIEHIDRLLFDQTKGQLAFKTARALMRLDEDPCEAILYVEFFGEDGDKLYELE
                     RRRLGRRTLICKDAVEQELVVSIRKQGLSLLSASKGPAKTIPGIEDACVVPARLPDYV
                     QGMRKIFDGLGLRGSFYGHAASGLLHIRARIDLHKAEDIAKYRKMAEEVSDLVREFGG
                     SLAAEHGVGFARAEFMPDQLGPDLMQATQDIKKLFDPQNLLNPGKIIPDGLHRIDTHL
                     RYGAGHRIELPFETAFGFIERDESFIGNLEQCNGCGLCRKDAPTMCPTFLATGDELMV
                     TRGRANIIRAALEHRTQNRDPLDSPELEAALSNCLSCKACKTECPANVDLALLKAEMN
                     HARQQRDGIPLVDRLISNAELLGRINAGLLAPVTNTILRMRLVRWLMEKTLGFAADRT
                     MPPYTRHRFDKWFARRPNKRKGVRGKVVLWDDCWVRYNEPNIGRAAVKVLETAGFDVV
                     LPDGRKCCGRPACSRGVLNMVKDLAHHNIDLFRTRGGTEPIIFLEPSCYTMLIDEYRQ
                     LRIPGADQVAARCVLFEQFVFELLQRRPDAIPFKDAGLKIAIHGHCHAKALTDVTIMP
                     KLAEKIPGASARLLDTGCCGMAGAFGMLKNKQELSRKVAQDLLEKINATEPGTTLVAS
                     GTSCRHQINHFTDRHPLHMAEVLASAI"
     gene            29059..31119
                     /locus_tag="P5316_03915"
     CDS             29059..31119
                     /locus_tag="P5316_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR84134.1"
                     /translation="MLRSPESRCPLYRLSLTIAGCLLLGILVSPTTAANETPLWSIAW
                     ISDTQTPACEWVTALCEKVKADRPSMVIHTGDVRFEWANQCAWKDVLNLLRISSPPIE
                     FHLAPGNHDLTNEVLKNHLRRAATRGIYLLDTGATAEGQGYYHSRFTTEISGPSWPIW
                     NPEVAVHPAWQPDAPQAPYRYVFKRGGIRFIVCDCYYTDEQRDWVRDLITQPDDSSVT
                     IILQHKHEVDQLAKYVEGLDGKHNVKLMLSGDHHNYCYEQRHGVTFITSAGMAEGANR
                     DSDAFTLRVYKDRLKLDRYLLPKGKPVTAIEGPTTVWECEGRFSEYRRPEYPVAIVAS
                     QPAPAASEAAVPTPTLAPNLLFNGNFDNHIWYERYRGWSPSGWYQWFTCGGHAPEHAV
                     GNDIPHSGKEYVRIHMWAHAWRGGVLQNVRGVEPCHWYRLTAYGWFAKTVENPDPKAR
                     IGLDPLGKLREQYGVNVTSHPAPAYNECVGDDPKTAGPDWPDIPESTVWSDYHDYDKW
                     GFFEVMAEARSDVVTAILYCDPRQRTANEPIYEMNWDTVALREIPWPTRRLAPENADI
                     PVEQSIKNMILTIQRKLGTAQLTWDTPVPCGASQVLYRFLDSEAVNHLPDGGSPKRIR
                     VDQFPFETPVAYERSEKCHGMEIGPLTIPDEAEELQAVALSRAVINGECRTLCSPLAR
                     FKLR"
     gene            31161..32171
                     /locus_tag="P5316_03920"
     CDS             31161..32171
                     /locus_tag="P5316_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR84135.1"
                     /translation="MKSFPASLKACLDRHPAGPIVLALVIACVLAVTVGCNTPATRFV
                     VISDLHPAPGAFDQLEAMTDHVIRLRPAFAVVLGDIGNDEVPGVSEEEIETIRKSFNR
                     MTAAGIEIYPVMGNHDVHHRVQDIKVNWFLAQTPRPLNHLFNQSAQTPAYQAFQARGP
                     YNYSFNRGGIHFAVVDSNISPPRPDWPAERLARERPQWEAHLQWMKDDFCRHVNNPRR
                     LPTLVFLHHPEYLTGDRGMDSRPLGRVLEECRDEQTVMAAFGGHWHGGANLAPKWKPN
                     LHVYATQASVHPATRPVEFIVAEVTAHGLTLEPRDSVTGQPGTGPVKYYPIAGRFTDL
                     RR"
BASE COUNT         6308 a         9999 c         9763 g         6229 t
ORIGIN      
        1 aatcctcgcc gaggatccac ttgggggagt ccatattgag cttctctcgg aatgccttga
       61 aagcgatgcc gtgcttggac tcctcataga tttccttctg cgggtgatcc ggtcccatgt
      121 ggcacttgcc gcaggcgtcg gggtggcggg cctgccaggc ggagaagctg tgccggctgt
      181 gacaggccgc acaagagccc tccgagccgt ccgggttgat gcggccgatg cccgtgttgg
      241 gccaggtggc ggcatcgagc ttgccgccgg gaagcacctt gatctccgcg ccgtggcact
      301 gccagcagcc gttgaccacg gcggcgctga ccccgtccgg aaaggccggt gtcttcatcg
      361 cgttgttgcc ctcgacgatc tcggccagag tgttgtccag cgagccgaga atacggccgc
      421 cctttgagtg gtgagactcg gcgaactcct tggtttcctt ctcgtggcag cgggcgcaat
      481 ccttgggcga cacgatggtg gcaatcaact ccccctcgtg tttgaaggcg tccgactcgc
      541 cctcctgcgc tgcatgacac tcgtaacaac cgacgttcgc acgatagtgc ttgctcgaac
      601 cccattgctg atatatcggg gtgttctccg tccggtggca ttcaatgcac gccttggtct
      661 tggcggacag ttcgggcagg ccccaggggg cggccgagac ggtagtcgtg acggccagaa
      721 tggctgccac ggcggctccg gcaagtgtct ttgcgatact catgtgatca tcactccttt
      781 tcaagcagcg gtctcggctt gcgaccgatt gttatcctga cgcgatggac cgtacagcct
      841 gcgataggtc agctcgccgg acagaaccgg caccgacatg cgcacaaaca ccgtgtaaag
      901 caaaagaccg aatgcccaga tgccgaggct gaccaacacc tcgttgatcg tgggcacgta
      961 ctcgacgatt tcccccagcg gcgtgggaat aaacgccgga acgatcaggc ccatcccctt
     1021 ctcgatccag atccccacga tggccaggac gcacgccacg ttcaatacct tgagaccccg
     1081 agaggaaggc agcaccaaca gaagggtcgc caccgtgttg aacgcgatgg ccgtccagat
     1141 ccatgggacc agcgcgttcc gtccgtgcag accgagatac aagtaacgtg aggaagcggt
     1201 gtgggcggaa tctgagtaga actcggtgaa cagttcgcag cccagcagga acagattgat
     1261 gagggcggcg acctggacga tccgccggag ggtgagcaga gcctcgtcac cgacgcgata
     1321 cgaggtgtat cgccggacga tctgcagcgt caggatgatg aacgccggcc cggcggcgaa
     1381 tgcactggcc agaaaacgcg gggcaatgat cgccgagttc cagaacggcc gtccgcccag
     1441 gccgacgtaa agaaatgccg tcaccgtgtg gatgctgacc gcccagacga tcgcgatgaa
     1501 gacgaacggc acgtaaaaag ccctggacgg cttgcgctcg cagtaggcgc agtagatcag
     1561 gtagccgcag atgtgcaggt tcaacagcag gtagccgttg agtgcgatca cgtcccaggt
     1621 caacatggaa cctgggaagt tgaaccgctg aatgagatgc agcgagcgat cgggacggcc
     1681 gagatccacc gtgacgaaca acaggcacat gatgatgacc gccacggcca atagttcgcc
     1741 gaaaatgacc acgtcgtgca gatgcatgtt gcgatagatg tagaccggaa tcaccaacat
     1801 ggccgccgcg gccgctaagc ccaccaggta ggtgaaattc gcgatataga cgccccagga
     1861 cacctgatcg gtcatgcccg tggtcgccaa accgccgacc agttgcctgc accaagcgtg
     1921 aagccccagc agcgcgaaca ccgtcagaac gctcatccat gcgtagtaac gccagtcgcc
     1981 gacgaaactc atacgcaggc actgccagag gaaggtgaaa taacgcctca tgcgtcacct
     2041 cccgagacgg ccggatccaa cggctcgtga tatcggctgc cgcgctcgtc gaagtagtag
     2101 aagaacctcg ggagtgtccc gacttcctgc ttgagaacaa acactcgctt gtgcctgagg
     2161 atataagcga tctcgctctt ggggtcgaga acgtttccga atttgcggga cccggttggg
     2221 caggcctcga cgcaggccgg catgcggccg acacgggtgc ggtgcagaca ataggtacac
     2281 ttctccatga cgcccacggc tcgcgggcgg ttggacaggt aagccatgtt ggggttcaac
     2341 tcttccgcgg tcagccccgg ctcggcgaag ttgaatctcc tggcccagta tgggcaggcc
     2401 gcctcgcaat accggcaccc gatgcaccag tcgtagtcga taaccgtgat gccgtccggc
     2461 tcctgccagg tggcttcgac cgggcacacc ttgacgcacg gcggattggc gcactggtga
     2521 cactgcaccg gcatgtagta actctcgtgc tcgggtaccg tcggcggatc gtagtgatgg
     2581 tccgaggtct ccacgtcgat gctgcccttt tccatcttga gcacgcggat gtactggatc
     2641 tcgggcgaac gggattggtt gttctctttg acgcaggcat gcacgcaacg acggcacccg
     2701 atgcaacggc tgatattgag ggcgtacacg aattccacgc cggtcatcgg tttgatgtcg
     2761 gctacctgcg ggcggacggc gtaccgtttc tcaacctcgc gagagatccg ctcgaggact
     2821 gccttcattt cctcgggggt catctccttg taatgcttct gcaacagttg ttcgagagtc
     2881 ggtatgtggt ccggtgagat gttccgcaaa ggcgagagcg cggccgccac tgccgccgcg
     2941 ccggcagcgg caacggcgct cttgcggatg aaaccacggc gggtcattcc cggagagccg
     3001 ggcgctgcgg cctgcggttc gtcctgcctg tcattcgggc cgttcatgcg tgacgtctct
     3061 gcctgacggt gtctggcgat agatcaacaa cgggttcttg gtcttctcgg tgcccgcagg
     3121 atgatgcgtt tgatcgccca tccgcagcgt attgggtccc cgggccggcc gcatggcctg
     3181 gaatgccgga gcatgcggat cgtgacactc gacgcatttc aggcgccgcc tcgatccgag
     3241 cgagtcattc cagtagccgt cggtccgccc gtggacgtca tgggtccaat cgcgatagac
     3301 cgggccatgg catttggcgc acagcaccgg cggctgatcg aaaggaatgg ggtctccccg
     3361 gtcgtcggcg aacgcattgc ggtcctgacg gttgtgacag ttgaaacagc gggcgttaat
     3421 gccgtggtcc agcacaatgt ggcgatgctg cgtcaacgct cgatccgtct caggaggcga
     3481 agggaacaga tcgtgacact cgctgcagcg gaaggtcatg ccggcgatga cggtttcagg
     3541 cttgagagta ggatgccgta cgggcgtcgt atcggtggcc catacgggta catccgtttt
     3601 aggtgggatg ggcacggcct cttgccacgg gtctgccaac aacaagcccg ccatgacaac
     3661 caacacggcc ggtacaaacc aaaacgcccg gcttcgtgcg ggaccagacc gtccacgccg
     3721 cgttgattcg ccttcgacca cctgccaact cccgagacaa cccgacgggc gagtcccctt
     3781 ataccgaccg ctcccgactc atgtcaaaca acggaataca ggcgaccgtc ttcagaatgc
     3841 aggcgccgcc gcgcacgaaa aagagacagc aagaatgtgc ccgtgtatca ttcccttacg
     3901 gccgatcgac gctctcactc gaagcccgtg agagactctg aagggtccga cagatcgtat
     3961 cggaagcacc aacaatgaag cgtttcgatc aaatcctgct cattgccagc ttcgttgctt
     4021 tctcatggct tgccatgcag gccgtccatg aacttgggca tgtgctcggc gcaagcgcaa
     4081 ccggcggcac ggtgaccaag gtggccctgc acccattggc cctgtcacgc accgatgtgt
     4141 cgcccaatcc ccaccctctc ctggtggtat gggccgggcc gatcgtcggg gcgcttgtgc
     4201 cgctaatcgt tttcctgatt gccgccgtgc tacggagccg tcgcaaaccc gagattgccc
     4261 gatgccccag aggggcacag gtatgtagcc aggggcgcaa gcccctggga gccgtgacgc
     4321 gccggccaac cagccccaga ggggcgacag ttccctcatt cccagatgta ccgctcgtcg
     4381 tagggtatct cgtgtcgccg gaggaactcg aggaattcct cttggaaggt catttgctgg
     4441 tggtgcttct gctgagtcga aatataccgt cgaacactgt catcgttcga tcggctcaca
     4501 gagaatgcgc catacccgct ttgccatgcg aacgcgcggt gctcggcaaa cgtttcgtgt
     4561 atccattttg aggagttggt cttgatcacc cgaagaacgt ctgccaccga aggatgcgcg
     4621 gggaaggaag ccagaagatg aacatggtct tcggttccgc ctgcggcgaa cagatggccg
     4681 tgctcgccct tgatgattcc gcccatgtaa tcgtaaaggc gctcgcggaa atcggcggcg
     4741 atctgaggca agcggttttt cgtgctgaag acaagatggt aatgaagaca cgtaagcgtc
     4801 tgtcccattg tcgtgtctcc tttttgtcgc ccattttggg gctcgttggt aaaaacggta
     4861 tttgtaccag gcccttgcgc ccctggctac acacctgcgc gtcttcgggg caggatgggt
     4921 aaagtgcgtg taggccactc cctgcaggat tcaatcggac aaactgctaa gcctcggact
     4981 tgggattgac ggatctcaga ttcagatccg ggcctggacc gcaggccaaa acccctcata
     5041 atcttggcca tgcgtaccgt gtctatcgtt gggtgtgcga gctatgaggc ctctgtggtc
     5101 gacgccgccg ttcggcagtc gctcgattac ctgggcggca tgtcccgatt cgttcggccc
     5161 ggcatgcgcg ttctgctcaa gcccaatctg ctggccccgc ggtcaccatc tgaagcgacc
     5221 acgacccacc cggccgtcgt ggcggccgtc gcccgactcg tgcaggaggc cggcggcatc
     5281 gtgactgtgg ccgacagtcc gggtggccct ttcgtggcga gttatctgaa aaccgtctat
     5341 gccaagacgg ggatggagca ggtggcacgg gaaaccggct tggcactaaa tcacgacctg
     5401 gccgccaggc aagtcgccaa cccttcgggc aagctgctca agcacgtgga catcatccgg
     5461 gccgtcgccg aggcggatct ggtcatcaac ctgccgaagc tcaagaccca cggacagatg
     5521 gtctataccg gcgcagtgaa gaacctgttc ggcgcgatcc cgggcacggg taagatcgac
     5581 ttccacctgc ggatggcgga ctaccaggca ttcgccgatg cactgatcga catctgcctg
     5641 gctgccaagc ctggtttgaa tctgatggac gccgtggtcg gcatggaagg cgcgggtcct
     5701 tctgccggag atcctcggca cctgggcctt atcctggccg gcgaagatgc ctttgctctg
     5761 gactatgtcg ctctgcaagt ccttggcgcc aatcccattc ggataccaat catgaaggcc
     5821 ggcaccgaac gtggcctgtg tccggcaagc cttgacgacg tcaccatcat gggccggtcc
     5881 gtcgaagacg ttcgcgtcaa acgattcgac atgcccgcgc tgagccaaat gcttacgatc
     5941 acctggtcgc aaggacggct gctggggcgg ctggcgcagt ggaccaagcc tcgccccgtc
     6001 ttcgatcatg tggcctgcac gggctgcagt ctatgtgcca ggagctgccc ggcccacgtg
     6061 atcgcgatgg agaccggcaa gccatgcgtc gatctcaagg gttgtatccg atgcttctgc
     6121 tgccaggagc tttgtcctgc aaaggcgatc acgatccgca gattgagacc atctgttgcg
     6181 gcgattctca ggatcgtgta cttcacgctg tccatgatct cttccagact gcaccgccgg
     6241 aaaaggcaac cacgacagga ttgagcgcag aagggcaatg accgcggttc gctgccggca
     6301 gttcaggatg cacactcaat ccgcgcagcg ggggtccggg tgcgcctccg ggccgctgaa
     6361 gcaacgctgg agccgcccga aatccgcctg atccacatca ccgtcgcgat cgaggtccgc
     6421 cgcgcggcag cactcccgca cataaggtat tcccggcccc gtggcgcaag cctcgaaacg
     6481 ttccagatcg tcgacgtcca catcccggtc aaaatcgagg tcagcccgcg gccgatcgtg
     6541 ggcgatgtcg tgtgcgacgt gcagcagggc catacagtgc ttgacctcgg ccagcgggtc
     6601 ggacggcgag aagttgcgga acaccagcca gattgggtcc gagctggcga tgcctgcggt
     6661 gttggtcagc tcgatctcga gcagatagac accgtcggcg gcctgaacgc catcctggtc
     6721 ggctgggatc gagttgccat cggggcccag caggaagtag ctcaggtgcc ggtgaaagga
     6781 cccgtcgctc ggcaccgtct ggaagttgaa cccttcaacg aagccggtcc ccccgcccgc
     6841 atggcgggtc atgaaaccgt atttgatcct gagccgctcg ccggccggca ccggcccgaa
     6901 actgaccgat ccattgccat cccagtagag caggtccgac agaatgttga agccgacctg
     6961 gcttcccgga ggcaaaccgc tgtcggttgt actgccatag cacggatcgt ccgtccagaa
     7021 cgggtccagc gggttctctt gaaagtggcc tccgccccag acatgcacgc cgatgtccac
     7081 cgtcaccgga ttggagggat catacgcgta cacgccggtg tgaatcttgt tatcctcgac
     7141 caccatcgcc acccccagat gcccggtagg atcgcacgcg tcgcagcagc cgcaggcagg
     7201 atccggctgc ccacatacca ttatcaaggc tgccatcatc attgcgagac cgctcgttcg
     7261 attcatgttt gcacctcctc catcgtctcg aaccccaagg cggcgtgccc gccgtgccgg
     7321 gcagcatgat ctccccggca cggcggttca cctcgtatcc tcaatgtgaa tcaacggcct
     7381 cgccgtcgcc gacacggcag cgcacccgcc acaagcaaca gggcgctcag cggttcgggc
     7441 acgagattgt tctcgaccca cgcagccgcc gcttcgtggt ttgcctcgct gtcaccgttg
     7501 ttgaagacga tccatatcgg ggcggacgat acgacgtcac tcatcgttgt ggtcagttcc
     7561 agctccagca ggtaaatccc agggcttggc tcgacgccgt cgccgggaat cgggttgccg
     7621 tccgatccca gcagctcgaa cgtcatgtgc tgatgcaacc cgccgccggc tccgatctgt
     7681 gcgaagttga agccgccggc cgggtcgttc gcgacattca cactctgcga accgacggtg
     7741 atctgcagcg actcgaacgc gggtacgccc gcaaagccgg agccgttcca gaatgccagg
     7801 cccgacaggg cgttgaaccc caaccagtgc cctgaaccgc ccggaaaggc cccgtcctcc
     7861 gaaaggaaac cagggtcttc ggtcatgaac ggctgagcgg ggtcgtcttc gccgaattcg
     7921 gccccaaaaa cgcgttcgcg gtctgaaaga ggcaccaccg tcgagccgac cagctcggac
     7981 gatccgaccg cgatcgcccc gttttgaacg taggcacgaa cgtcaacatg tgcggccagg
     8041 gtgacgtttc cgagcagcac aacgataagc actgaagaga taaacacgtt cttcatttct
     8101 gaatccttcc ctcctgtttc gcaggagatc taaatgagac gcctttcggg cccggtcatc
     8161 gacggtgata gttgatgaac aaacccggat cgaacttgtt atgctccata tccttgtaaa
     8221 cttgctggaa ctggagcgat tccgcatgtc cgtccagaaa accatagttc gacagcgaat
     8281 gagccgacca cttggggccg ccgtgggcct ggatttccag ttgcgtcgcg gccttggtcg
     8341 gcgaatggct cacgtcggcc cattcctcaa cgtggggatg atcggacgcg gcgaagcatt
     8401 cacccgaccg gtaataggga ttgtccggat cgcactcctc gaccatgtag acaaaatgca
     8461 cgatgctcga ggggttgcgg atcttctcga tcttgggcca agtgacgccc ttgccggtca
     8521 gatccggatt cttctcgatg tcgaggaagt tgttgatccc gtaactgctc cgccggaacg
     8581 gatagcctcg cttgccgtcg acgggtatcc ccttcccgcc gagttccacg ggccagtggg
     8641 gactatcatc cgccggagag cgggccacca gccgatcctt gtagtagcgc tgcagcgtgt
     8701 tgaaccaggc cacctggggt ttgtcatccg ccacgccgtg cataagaccc acgcggatca
     8761 ggaatccgtc gttggacgtc atgtacagcc agtgggcggt ttccaggcac ttgatgttgt
     8821 tgagacacat cgtgcgccga gccgcctgcc gcgcccggct caaggacggg agcaacaccg
     8881 ccacgagcaa ggcgatgatg gcgacgacaa ccaacacctc tatcagcgtg aacgcgctgc
     8941 ctcgcagacg cgtcttggag catgctcgca tagcgcacct cgcatatgcg tccatcactc
     9001 gctgtgtacc atggacctgt agttgtggtt tgagccgccg cgtctgcgag acgaaacggc
     9061 acggaacgtt gcaccaagcg ccggccatac gccgtttccc ccgtcggaat cgcaggtggc
     9121 agaaacgccg tttaccggca aaggatcaaa gaaaactggg agggcctcgc ggcagactcg
     9181 gcgcgggacg agtcgttggg acgaaatgtg cgtcgctcag aaccacccgc tcctcattcc
     9241 gatcggcgag cagcgggata accgaaatcg ccggcggaat caccgccggc gtcaggatcg
     9301 attgctggca tagtgcgcac ccggccctgt catgctggtg cccctcttca agccgagaat
     9361 gaacccaaag ggccgaaccg ctcgaggaaa gcgccagaca cgccaagagc agtcctgaaa
     9421 tcaaagatcg atgaaaccgc atacgtgtat tacattgtaa gcgaggccgg gcaagtttca
     9481 agctgcctgg gcgcggcccg ggacaggaag acgtaaagat cttcagtgaa aggatttacg
     9541 gttttgttga acagccgatg actggttgca tggaacttgg tctggccgcc aacggtactc
     9601 gcgcaggtct ccaggggcag aggtggccgt cccttgcaac tggtgagaac cttccggtcg
     9661 gcgcgcgctc ccaatcttgc tttcctctgc cggaaagccg attatcttga cggccacgcg
     9721 catacgtatt atcttttgat aattcgtaat actacaggcc ccggtctcgc gttgactccg
     9781 gcagcctgga gcggaaggaa caaggagaaa acggatgaat tcccgaattg cgcgatggct
     9841 tgcgactgca gcgttgttct gtgcgtggac gacggcgaca accgcacagg agccggattg
     9901 ggcatcgatg agttacacgc tccatcagga ctatcaagcc gttgatgcca acggccaagg
     9961 caccttcccc accacctcac ccgtcaagat caaaggcgtg atcctgaatc gtccgtccga
    10021 catgttgaac cccacacccg ggttcgatcc ttacatgggc gggcagtggc aacagtttgt
    10081 tcaattcgtt gatgaaggtg atttcggcgg caccgcgttg tacatgggcc agaacatcgg
    10141 caagaacgtc gggaatcatc cggccggcag ctacagcgac gcggagtggc tcgccgagtt
    10201 gcaccgcctg aaccacgacc cgatcaccgg cagggctttt cggtcgggcg atctggtcga
    10261 agtgcgagcg cgggccccgg gcctgttctt ccgcggcaag accaacgtca acgagcaaca
    10321 ccagaaaacg ccactggcgg atttcgacgt ggttcttctg caagcgaact atgtctcgcc
    10381 tgcgccccaa accatcaccc ttgcggacgt aaaggatacg gccgatcagt tcatttttga
    10441 tcagtcgcgt gccacgggcg cggagcatta ccagggcagt ttcgttcgat ttgagaacgt
    10501 gcagttcgtc gccggcacct ggggctgcaa tcaatcaatg accatcgccg acgacaccgg
    10561 gcgaacgctg cccgtcctgc ttggattcgg cggcggtttt acgcagtacc ccgcaccgac
    10621 cggaaccttt gatatcgtgg gcatcttcga tcaagaagac aaggtctcca ccgacggatg
    10681 gaagggcggc tatcgactgt gggtgatgga ctacgacggc agccatttcg tgctctatcg
    10741 gtacgtgaag cccgatttcg accgagatgg cgacgtggat gagaccgatc tcgatcactt
    10801 cgagacctgt gcctcggcac cgggaatccg gcagaccgat ccatcctgcc tcaatgctga
    10861 ttttgacgaa gacggcgacg tggatcagag cgatttcggg gtgctgcaga ggtgtttctc
    10921 cggaagcctt gagctgccgg actccgcctg tgatgggtaa tgttgttcgg atggcgcatg
    10981 acagaaggca agttcgatcg acccgacccg gcggcggccg ttgcgcgcga aacggcgtcg
    11041 tagaagcctt cagtctggtc gaggtccttg ttgtggttgc gatcctggct ctgctggcgg
    11101 ccatgctgct cccggctctg gccagggcaa agcttcgatc cagggtcgtg ctggtgcact
    11161 ccgacttgcg gcaggtcacc atcgccctgg acgcctacgc cttggaacac aaggaccgtc
    11221 tgccaccgac gcggcagtct tgcgggacga gcatccttta tcagttgccc tttgagctgg
    11281 ctacaaagaa gtacctgccg aaaagtccgg acgcaattaa gcgatcatat ttggaggacg
    11341 cgttcaaccc cggtcagacg tatcgttatc gggccccagg accggtttgg cagaatggcg
    11401 tgctcatgga ttttccggat agtacctggc gaccccgcgc acagatatgg gtgcctgatg
    11461 acgtgccaaa ttgcgaaagc cccaacggca ggtactatgc tgatcgcaat aacgaaccca
    11521 agagtcccgt gacgtatgcc gtctggagcg tgggtccgga tccgcattca cccaagtttc
    11581 cgcgctggga cgacggcacg attgacgagg ccaaactccc cttgcccaag tcgttctggc
    11641 tgctcaacga cggtcgtccg acgggcctca tcacccactt caggacccgc aaaggcgtga
    11701 ctcacatgag cccgtgatct gccgttttgc ccccgggatg atgacaaggg cgaatgatgg
    11761 agatggtcat caagacgcaa accgcgataa tgtgaaaggt gcctcgcagg catctgcttg
    11821 attggcgaga tcggaggaca agtggatttc tacacgaaca gacaggcgga agatcctgat
    11881 tggcttgcac gggcggcact ctttcacggt cacctgggac catggttgac cgttggggcg
    11941 atgatcggac aggatgccat tcgcaggctg gaaactccgg ggcagtggaa gatccacgtg
    12001 acctgctgga tgccggccga caagcatcgg acgccgttca cctgcattct cgacgggctc
    12061 caggctggtt gcggggcaac catgggcaag cggaatctct ggctgaggga ttcggctgaa
    12121 gtgctctcca acaggtggcc tgtggtccac gtcgtcaggc tacccgacgg tggtcggcct
    12181 gcggaggggc tcagctatac ggccacctgc gccctgcatg ggtggatgga acacctcaag
    12241 cccgaacgtc tggaggagga ctcgcgggcg ctggctcgcg aggacgtaac gaagatgttt
    12301 cgcattacat caatgggcga gagagagttt gcctgggttg gctgctcctc agccggccag
    12361 gtgcgcgcga cgacaccatg acccagcgaa ccgccatcca agcagtcggg ttgctccatg
    12421 tctttggtct ggctgtaacc ctggccggct gcaaccggtc atctccgccg caaagcccgc
    12481 cggcgccgaa cccccttgcc catgtcgcgg ccggcaactc ctatctggaa gcggcattga
    12541 tcgatctgat gggcgacgcc tcccggccgt tgcgtctggc cgaaccgggc acctgccccg
    12601 gccatttcga tgttcgcccg agccaggtat cggcgttgag gacatgtcgc atgctcgtgc
    12661 ggtttgattt ccagcgagct ctggacgaca agctcagccc tcttgctcgc gacggcttgc
    12721 ggatcgtgcc cattcgcgtg tcgggcggat tgtgcatacc ttcgagctac ttatcggcct
    12781 gtcgccaggt ggcggagccg ctcgtggccg cgaacatgct ctcggccgaa gcgtcgcgac
    12841 accgaattgc agagattgag caacgaatga gcgagcggga ggagtggtgt cgtcggcaga
    12901 tgcgtgaggc cggcctggag ggaaaacctg ttgtatgtag tcctcaccag gaagccttct
    12961 gtcgctggct ggggttgaac gtggtctcca cgtttgcagg cgccgattca gcatcaatcg
    13021 gcgacattga gaccgccatc cgcgaaggcg agaatgcccg cgtcgggatc gtgatcgcca
    13081 acttgcccga ggggcggcgt ttggcggacg cactcgcgca gcgactcgga gcgaaagtcg
    13141 tggttttcgg taacttcccg caagacgacg ccggaacgat ttctttcgac gcgttgatcg
    13201 cgaccaatgt tacttcgctg gtgaaggcgg ctactccttg aatgatacgg caatcacgat
    13261 tcgcgatctg atcgttcggg ccggacgcag gaacatactg gcgatagaga atctgcaact
    13321 gcccaagggg caattcgtcg gaatactcgg cccgaacggc gcgggaaaga gcacgttcct
    13381 tcgctgtctg ctgggtcttc aacgacatgc cggcggacgg gtaaccgtgc ttggcgaacc
    13441 gctggccgaa ctgggcatgc ggggcctcgg attgctgcga cgtcggatcg ggtacgtccc
    13501 gcaattgctt tcctcgcccg gggagattcc cttgaccgtg cgcgaggtga ttgccatcgg
    13561 gcggaccgga atcgccggcc tgatccggcc gctgcgaaag aaggattggc gaatcgtcga
    13621 ccaatggatc gaacgtcttg ggctcgcctc cctgactctt cgcggctacg gtgagatttc
    13681 agggggcgaa cagcgcaagg ttctgatcgc ccgggctatg gtccagcagc cggatctgct
    13741 gctgctggac gagccgaccg ccaatctgga tctcggctgg cgcgagcgaa tcgtgaccgt
    13801 tgtcaaggat ctctatgcgt cgtccggcat gacggtcatt ctggtgagcc acgacctgga
    13861 agtactgccg ccggcctgcg gccgggtgat cgtcctggag aggggccggc tggtggcgga
    13921 cggctcacca gacgacgtat tgactgacca gcggctggaa acgctttacg gtccggggct
    13981 gacggttctc aagcgtggcg gtcgactgct ggcggtgccg gcagaggttt tcaatgcttg
    14041 attttgtctt ctgggcaccg gtgctggtca gcgggttcct ggccggctcg tcaagcggcc
    14101 tgctcagcgt cctgatcgtt ggcgcgcgca ttccgtttct gggcatttgt gtcgctcacg
    14161 ccgcgttggc aggagcggtg ttcggggcgc tcgccggctt ggagggacag ttgctgctct
    14221 tgccggcggt ggccgcctct attgtgaccg ccgtggtgct gggcctggcc gacccgcgaa
    14281 aggttcgcat ggacacaaac gtggtgctcg gcctgctctt ctcgctcacc atggggctgg
    14341 cctttctggg tttcgggctc tttgccgtct ttggccgaag cgatgacgac gtccgcggtt
    14401 tgttgtgggg aagcttggcg tactgtcgat gggcagatgt ggtcctgatg accgtcgtat
    14461 cggctgcgct gctggctttt gtcttgcttt tcggcaagga aatgcgggct atcgtcttct
    14521 cacgacagga tgcgctcgcc gcggggattc acgcgacggc ggtctggacc ggcttcctgg
    14581 ttctcgcggc cgtactcata accgtcaact tccagaccgt cggcggtctg atgatctaca
    14641 gcctcatcac caacccggcc gcggcagcct ttcaactcgt gcgaggcatg cgaaaagctt
    14701 cggtagtcgc ggcgctgctt gggggcgtca gcggactggg aggattcctc atcgccgctg
    14761 cgaccgacct tcctgccggg gcggtgatcg tcattctttc atcgctgctt gtagcagggg
    14821 caatactgct gcggcgaatc agagaagcac gataggcagc cgaccgtctg cacatcgcct
    14881 cttccgctcg atggacgagt gcaagccaat catcgccggg acgttcacgc cgtgtggagc
    14941 gggtgaagcc gcgatctgca tcgtttgacc ggacgcgcaa gtctccctta ctctgcttga
    15001 gttttccctg agcaccgtcg cagcgcacag gatgtcgcat gttagccggt gcccggggtt
    15061 cttcggaaac aacaactcgc gagcccgatg gcggttgaaa aggaggcacg cgtgagtccc
    15121 agccacgagt cgtcaggcat tgccggccgt ctggataaac tctacgaatt cgagcgagcg
    15181 ccggtcacgc cggacaggtt gcacgacgga agatacttcg ccggtctatt tgccggcgaa
    15241 cacgtcgccg caaccgagtt cgtcattggg gcgttgttcg tccagtgggg agccggcgcc
    15301 cgcaatctgg tcctcgggct gctgctggga aatctgcttg cggtgttgag ttgggccttc
    15361 atctgcgccc ccatcgcggt ccgcacccga ttgacgctct actggtacgc ccgccggatc
    15421 atcgggccgg gcctgacggc ggtctacaat ttcatcagcg gcatcctcta ttgctgcctg
    15481 gccgcggcca tgatcggcgt gtcggcttcc gcggtcatcc ttggcctctc acgattgggc
    15541 ctgcacgtca ggcatcccgc actgaccgac gtatgcccca acagcgttgg atggttggtg
    15601 gtcgtcttga tcgtcggctc ggtgacgacg atcctggcga tcgccggctt caagagactc
    15661 agccagttcg ccggtgtctg ctcaccgtgg atgtttctca tttttatcgc cggtgcgata
    15721 gcgacccttc ctcgtctggg cgacgtgcgt agcctggatg acttctggag aatcgccgag
    15781 acgaggatct ggctggagac gcccaaggcc ggcaccgagc gacttggctt ctggcacatc
    15841 accctcttcg catggttctg caatctggcg atgcatgtag gcctgtccga catggccatc
    15901 tttcgttatg ctcgccgctg gacgtatggc ttctattcgg ccttcgggat gtacctgggc
    15961 cactacctgg cctggatttg tgccggcatc atgggtgcgg ccgtcagcga ggggctgaac
    16021 cccggcgaga tggcccactt ggcggcaggg ctgccgggcg tattggccgt attgttggca
    16081 ggctggacca ccgccaaccc cacgatctac cgcgcggggc tggcccttca aatcgtgaca
    16141 ccgaattggc ctcgctggaa ggtaaccttg gccacaggag caataaccac actcgtcggc
    16201 ttgtttccgg ccatcttcat gaagctactc aatttcgtgg ccatctacgg tctcgtgctg
    16261 atgcccatcg gcgcggtcat cttcgccgag cactggctgt tccccagaac gggtctacgg
    16321 caatgctggg tcgagcgtcg aggtctgagc gtcaacctgc ccgccctcat cgcctggctg
    16381 accgtgctgg tcctttgttt cccaattgag gatttcacgg ccgggcggct caggtctccg
    16441 atggatttga tgggcgtcgg gctgttcttc cgctggctcc cggcgtggtt tctgacggtg
    16501 agcgtttatc tcctgctttg tcgtctgacg cccggtgcgc ggcgacagcc cgatgaacag
    16561 gccgaaatca gccttgaggc ccggtcaatg atcaccgggc cggtagcggt ccggacgtcc
    16621 gcaccgttgc cgccgattgc gtggatcagc ggagcgctgg ccgtgatggc cctggcggcc
    16681 tgcctgatcc tgcccatgct ggtattcatc ggtggctcag atccgtccgc ctatgaacgg
    16741 aacatgggct cgtacaagac cggcctgatg gtcgccaccg tggcctactt catcggcggc
    16801 atcatctgga tggtccagcg ggagaaggct cgcctgactt gaattgtgcg cgtcccggca
    16861 gtccctggcc cctcggggct cctgatgttg tgggcggctt cttcagaatc acgagcgcgc
    16921 caccgcgaag cgtgtcgtag ccgggtaagc gaggtactga aacagaaagc ggccgcgcca
    16981 caagggaccg ctcacgtacg gccgcctcgg cgtaccagcg tctcggttct gaaggagccc
    17041 cctgaagaaa ccgtggcgtt tccccgccga gtctgtcgcg ggacggtccc atcggtcatt
    17101 tcgtatcgcg agctacagta tcgcagtgac gtgtggtata atatggtgat gaaaccgacg
    17161 gtatacatcg agacgacgat accttcctac tactgcgacg atcgcccgga attgatgggc
    17221 gacatcgccc gtacccagga gtggtgggac aaggaaagag gagactatga gtgctttatc
    17281 tcccccgttg ttatcgagga gctgtccgcc ggcaactacg ctactcgatc caagtgcctg
    17341 cgtctgatag actcgattcc actgcttgag gtgaacaggg aggttgcaca gatagccgcc
    17401 atttaccagg agcagaaact gatgccgagt ccgcccgtac gagacgcact gcatctatcg
    17461 ttggcggcct actaccggct cgattacttg ctgacctgga attgccgaca cttggccaac
    17521 gcaaacaaga taccgcgatt gcagaagctg aaccgggaat tgaatctgag catgccgcta
    17581 ctggttaccc cggccatgct gcaaagatgg gaggtcggtg atgagtgata gcccaatagt
    17641 ccaggaagtc cggcggcgag cgatggagat cagtgcacgg ttcgaccacg atttggaccg
    17701 ctatgtcgaa cacttgcgtg agtaccagga gcaatttcgt gaccggctgg tgagccagat
    17761 tactgtggtt cagtcgcctc gtcatggaca agaagtgggc aagcccaagt gactccgaat
    17821 cgtcgcgtgt gggccgcgcg gatgccgtgg tagcgcgggt gtgggctcaa ggaaagccgg
    17881 gtgcgacgcc gcccgattcg tctcgcgccg ttgctccagt gaatcatgac ttcttacctt
    17941 ctcttggcga ctctcgtgct cttgtctcaa gcggctttgc ctcaaagtcg accctcgtcg
    18001 caacagccgg gcctgcgcgt cgtcacctgg aacgtccacg gatgcgccgc cggtgtcgat
    18061 gggatcgtgg atgaactccg aacgatcgac gccgacgtca tctgcctgca agaagccgaa
    18121 gtggggacgg cccacgccga gggtgccgat caggctgccc tgatcgcaga gcggttgggc
    18181 atgaagcact actccgccgg ttcgcgattt gctgagggag gaggcgagca gcgcatggcg
    18241 attctcgccc gcggggagct ggagcagacc ggaccgctcg acgccggcac gggacgaatc
    18301 tatggtgtcg ccgctgtgac ccgatggaac ggcaggcctt tgcgtgtcgt atcaatccat
    18361 ctgaccagca cgtatcgcgt cgacctgcga cacgccgccg agactagtcg ggcgagattc
    18421 aaggaagcct cggatctggc cggccgaccc aagaaatgga cggaggatgt ggtggttgca
    18481 ggcgacttca acgccatccc cggttcgccc gaacacgtgg tcttggccgg cggactcaaa
    18541 cacgtcgcaa cgacccagcc gactttcccc agcgaccggc cgcgactccc catcgaccat
    18601 gtctacctct ctgacggtct tcgcgctgat cttcgggtcg ttcgcgattc gctcgcctcc
    18661 gatcaccgcg cggtcatcgt cgaactcacg gccgtgcgag gaccgtattc ccaaccggcg
    18721 gacacagccc cggtcagtca gggaaccggg tcctcaatcc accgacgata acagggtacg
    18781 cagaggtgga tgaccagccg ccgatgcacc tggtccatcg cttcctgttc ggaaagccca
    18841 tcgagacttt cgacaagccg gtcaatctcc gccctcagat cgcgtttgag gtcattttcc
    18901 gtgatcaccg gtggactggg gtcggcaaag gcatcaatcc gcacgacgta gaaatcgcca
    18961 cggccggggt gaagctcggc accgcaacga tgacaaacca tggccgcctc atcgctcata
    19021 accgccattg tacgtgagcc tccggctttt cccacggccc gtaataagcc ggccaggctg
    19081 tccgcccgca ctcatggcaa acgctccgaa tgaattgacc gccgatcagg ccgacgctat
    19141 tctcttcacc agagtactgc ccgctgaacc ggtcctggct gcccttcgtc gcgaggagga
    19201 ggccggacat gactgatgac agagtcggta acttgccgag cgaaagacgg gtagtgatta
    19261 cgtcgtaccc ctcctgggcc caagactgag gagaaaacca gaagccatga gcaaaacaaa
    19321 atctgtcgag aaagcctacc agcttgccaa ggaacgttat gccgagatcg gagtgaacac
    19381 cgatcgggcg attgccgcgt tgaagaagat ccccatctcg ctgcactgct ggcagggtga
    19441 cgacgtcggc ggcttcgaaa gcacggccgg cctgaccggc ggcggcattc aggccaccgg
    19501 caactatccc ggcaaggccc gcaccgccga cgaactgcga gccgatctcg acgtggtcta
    19561 cagcatgctc cccggcaagc accgccttaa cctccacgcg atctacctcg aggccggcaa
    19621 gaaggtcgat cgcgacgaga tccggcccga gcactttgcc aactgggtgg cctgggccaa
    19681 gggcaagctg cacggattgg atttcaaccc gacatacttc tcacacccca aggccgctga
    19741 cggtttcacg ctctcgcatc cggacgccga catccgcaag ttctggatcc ggcacggcat
    19801 cgcctgccgg aaaatcggcg aatccttcgg caagcaactc ggcacgccct gcgtgaccaa
    19861 cgtttggatt cccgacggct acaaggatat caccgtcgac aagaaggcgc cgcgcgagcg
    19921 actggtcaaa tcgctcgacg ccatcttcgc cgagaagatc aacccgaagt accacttgga
    19981 cgccgtcgag ggcaagctgt ttggcatcgg cgcggaaagc tacaccgtcg gctctcacga
    20041 gttctacctg gcatacgccg tttcccgcgg cgtgttgctc acgctcgacg cgggccactt
    20101 ccatccaacg gaggtaatct cagccaagct gtcagccgtc ctgtgcttta ccaaggaact
    20161 cctcctgcac gtcagccggc ccgtgcgatg ggacagcgat cacgtagtga tcctcgatga
    20221 cgagttgttc gccatcgctc aggagatcgt ccgcaacgac tacgccaaca ggatccacat
    20281 cggcctcgac tatttcgacg cgagcatcaa tcgcgtggcc gcctgggtca tcggtgcacg
    20341 aaacatgatc aaggccttgc tggccgccct gttggagccc accgaccgac tgcgcaagta
    20401 cgagcgggac ggcgacttca cctcccgcct ggccttgctc gaagagcaca agaccctgcc
    20461 gttgggagcc gtctgggact tctactgctt gcaggcaggc gtgcccgtcg gggccgagtg
    20521 gctggccaag gtcaaggact acgagaccaa ggtactcagc aagcgatagg aagagaagtg
    20581 ctcagttgtc agttgtcagt tatcagtgac ctgtgactga caactgacaa ctgataactg
    20641 acaactcagt ctcgccattt gggtttctgt aaggagcgat gcatggaaac caaccccgcc
    20701 ttaggcgtga tgatcttcgc cctcggcggt ctggccgggg cggtcttcta tctgcctttc
    20761 aagaaggtca ggaactgggc gtgggaaagc tactggatga tctacgcgat cgccggcctg
    20821 atcgtcgtgc cctgggtgct ggccctttca acctcgccga acgtggtggc cgtgttgcgg
    20881 cagtctccgg gaacggttct ggcatcctgt ttcggctacg gggccttgtg gggtctcggc
    20941 ggcttcacct gggggctgat gatccgttac ctcggcgtgg gccttggcct ggcgatcggt
    21001 tgcggactgt gctcggcgac cggtacgctc atcccaccca tcattcatgg cgatccgcag
    21061 aaactgtatg actcgacatc cgccgtagtc tcgctcatcg gggtggtcgt atccctgctt
    21121 ggaatcatcc tcgtcggaat ggcgggcatg tccaaggaga gtgaactgcc ggaggaagag
    21181 aagaagaagg ccgtcgccga gtacaacttc aggaagggta tcattattgc gatcttctca
    21241 ggcctcgcga gttcgggcat gagcttcggc ctccagggag gtcagatcac ggagtacaag
    21301 gcccggttcg gttcacagac gcagttggtc aaatcggaag actcggttgt tcctcccgcg
    21361 gatttggtcg gcgcggcagg catattctac gacaaggatg ccaaaacctg ggtcgctccc
    21421 cgtgcccctg agggcgaatc caccacttcc caaacctggc gcggcatgcc gatcctggtc
    21481 gttgttctgg ccggcggatt cgcagtcaac ttcctgtatt gcctctacct gaatctcaag
    21541 aacaagacct tgggtgacta cgccaagaag gacacgccct tggtcggcaa cctgttcttt
    21601 gccggtctgg ccggggcgat ctggtgctgt cagttcatct gctttaagac cggcgagccg
    21661 gcaatgggcc cgagggcata catcggctgg gcggtcctca tggccagtgc gattctcttc
    21721 agtaccctgc tgggcattct cctgggagaa tggaggggga cgagcaagcg gacccgcacg
    21781 tgcctggcgg caggcctggc tttgctgatc gtctcatcgg tcatcgccgg tattagcggc
    21841 tacctcaaga cggcctgata tccagccatt ggcaccattt tgctgatcaa gggcccgcag
    21901 tcgtggccgg ctgggtcgag gcatcgcctt tcggcaagcc gatctccgca acgaccaaac
    21961 gatggtcgga gccggtgtcg ggtatgacct tgccggtcac ggccgtcaga tcctttgaac
    22021 agaagatgta gtcgatgcgc aatcggatcg gtcccagttc ggtgtcgatc tgccaagtgg
    22081 gcgttccgct gccctcctcg ttctccgcgg gattggtgga tggcagcttg aatgcgtcaa
    22141 catagccctt ctccgccagc aggcgcggcc cggacgatgg cggaatgctg ttgaagtcgc
    22201 cggcgatgat cgtgggcagc ttcgggtcga tgctcttgag gatgaagccc agttccgccg
    22261 tgtgggcctt gtcgagcgcc ttgaaagcct ggagatgctg tgccgggcgg gcgttcggct
    22321 gcaactgcag cggcttgagg tgcacattga ccagttgcag ccgccggccg tccattacgg
    22381 tttgagcgac aagcgttccg aaaagcccgg accgcggggg caggaaccgg gtcttctcga
    22441 tcggagatcg ggccaggacg ccgaaacgat ccgtgggaat cctaccgccg ccgcccttga
    22501 aaacggtgta gggatagagg tccttcaact ccttgcgcaa gagctgctcg atcttgctcg
    22561 tggtctcctg aagaaacacg gcatccgcct tcgactcgcg aatggcctta acggccggtt
    22621 cgggcgaccg gttctcgaag tagacattga gcgtggcgac agtgacccgc cactcgtcgg
    22681 gcgccgcaaa cccactggcc agtgtcgacg cgggcattgt cgtggtcgtc gcggcaggag
    22741 cccgctatcc ggctccaaag cctgatgtca gcagtaccat gagcgcaaag atgatcatct
    22801 gtcactctgc gatacaacgt cggcacagga atcgggtccg acgccgatta atgctccgtg
    22861 cttgccgaca taccccaagc tgagagctga aagccaacag ctaatcgctt cacttcccct
    22921 tcttcttcgc cttcgtcgcc gcgttcaggt cgatgcccag ctcgtcaagc tgcttgggat
    22981 cgacctcgct cgggcactcg gtaagcgggc agaaagcctt ctgcgttttc gggaacgccg
    23041 tcacgtcgcg gatgctcgct gcgccggcca gaagcatcgc tatgcgatcc agacccaagg
    23101 ccaaaccacc gtggggcggg gccccatact gcagggcggt cagcaggtgc gtgaacttcg
    23161 tcatcgcttc ctcatcgctg aggcccagca tgcggaagat gcgactctga accccgcggt
    23221 cgtggatacg aatcgaaccg ctgccgagct cgacgccgtt gagcacaatg tcgtaacact
    23281 tcgctcgcac cgccccgggg ttgctctcca ggatcggcag atcctcatcg tgcggcatgg
    23341 tgaacgggtg gtgcatgaag acccaccgcc tctcatcttc cgaccactca aacgcgggca
    23401 tgtcgacaat ccagagaaat tcccagcggt ccttgggaat catctttcgt cgctcggcca
    23461 gcgtggtccg caaccagttg agatacttgc acacgtcggc aaacctgccg gccgcaaaga
    23521 agatcatgtc cccgttaccg gccccggtcg cccggatcac ctcgtcgccc aactcgccag
    23581 ggaaaaactt ggccgcaccc gtctgtagct cgagtttgcc gcttgccccg gccaccacct
    23641 tgcacaacgg caggccgccg gctccgatac cctggatctc ggcggtaaga ccatccgtct
    23701 cctttcgggt catctccgcc ccgccgggca ggcaaatggt tttgaccacc ccgcctctgg
    23761 caatcgcgtc ggtgaacacc ttgaactcgg tcttcctggc cacctcgcta acgtcgatca
    23821 gcttcaaatc gtaacggaga tccggcttgt cgctgccgta gtcacgcatg gcatcgtcgt
    23881 aacgcatccg ccggaacggc gtcggcacgt cgacgccgat cacctgtttc cagacgtatg
    23941 acaacgatcg ctccaccacg tcgagcacgt tgtccacgtc gacaaacgcc atctccaggt
    24001 caacctgcgt gaattccggc tggcggtcgg cgcgaagatc ctcgtcgcga aaacaacggg
    24061 caatctgcat gtaacgatcg aagccgctga tcatcagaag ctgcttgaaa agctgcggcg
    24121 attgcggcag agcgtagaac tgtcccggcc agacacgaga cggcacgagg taatcccggg
    24181 ccccttcggg cgtggatttg gtcagaatcg gggtctcgat gtccaggaat ccctcatggt
    24241 aatagaactg ccgaaaggcc tgcaggacct tgtggcgggt aatgatgccc ttttgcatcg
    24301 ggccgcgacg aaggtccaga aatcggtact tcagtcgcag gtcctcgttg gcctccacgt
    24361 gatcggcaac ttcgaacggc ggggtcagcg acttgttcag cacttgcacc tcgtgaacgc
    24421 tgacctcgat ctcgcccgtc ggcagcttgg ggttgacatt ctggccgcgg ctgatgacct
    24481 cgccgcggac cacgatcacg tcttccttgc gcaagctgcg ggccagcttg gtggcgtcag
    24541 ggtcagtgtc cacgttgaat cggcattggg tcagaccacc gcggtcccgc aggtcgatga
    24601 acaccaggtt gtcaccgtgg tcgcggtagt tgttgaccca accggcaaga ataacggatt
    24661 ttcctacgtc agaagcacgc aaggtgccgc agtcacaagt acgcttgttg tacgcaatcg
    24721 ccacggaaca tctcctcttg tattcaaacc ggaccgtccg cacttcacgc gccgacgacc
    24781 ggcaaaaaac caaccgaaag caagcagtct acggaatcgt gagccgccgg gccacagggc
    24841 ctcggcaagg gacggcgacc ggccttccgg ccggtgcttt tcaggcgggg tcacggacgg
    24901 ttgaagcggc gaccgagagc cccgcccaga cgcgcggcca ttcggtcgcc gatgatcctc
    24961 cgtatgcgtg aaagatccga tgccatgaca atggtatcct atcacagacc gcaaggccgg
    25021 agcaacgccc cacgcacagg gacggattgc cgctcggcac gtcctgagcg tccggaacta
    25081 ccgcaacgcc ccacgcacag ggacggattg ccttcttacc ccatcggtca aaacctgctc
    25141 ctcataatcg ccataagtcc cgtttattca tgagtttatc gtgagtcctg tcagacgaaa
    25201 tctcgggaac aatcagttac cggtcgccca cagactgaat tcccgatccc tgatctgaac
    25261 ccgccttgcg ttacccatgc agaagcctca taataaaagg tttggatccc gggcgggatg
    25321 gcaccccgtc cgcgacaatc aaggaatgac cccttaccgt tggtggcctt gcccgtgccg
    25381 gcgaaggtcc cggtttgggc gagctggtga ggtagtactg gatatggcgt tcaagaagtg
    25441 gacggcttcg gcccggcaac tgcggccgga tcctcggttt aacagcaagc tggtcagcaa
    25501 gttcatcaac tgcctgatgt acgatggcaa gaaggccgtg gcgacccgag tattctatga
    25561 tgccatggac atcgttgcca agcgggtgaa ggatcacagc ccgatggagg tattcgagac
    25621 cgccatcaac aacgtgaagc ccagcattga agttcgcagt aagcgcgttg gcggctcgaa
    25681 ctaccaggtg ccccggcccg tcaaccgcaa gcgtcagcag tcgctggcca tccgctggct
    25741 gctggaggcc acccgcggcc gaaagggcaa gcccacctgc gaggggctgg ccgccgagct
    25801 gatcgacgcc ttcaagggcg agggcacggc gatcacgacc cgcgagaacg tgcatcgcat
    25861 ggccgaggcg aacaaggctt tctcgcattt tgcctggtaa cgcggacacg catccagtca
    25921 acacggatca agactaaaga cggccgggaa acttcaacaa ggtcccggcc gttttcttgc
    25981 gctcgcactt cccatagaat agacgccgaa catgattgat tcagttcaag aaagactcag
    26041 ccggtcaacg gattgcgaaa ttcgatttga ccatttagcc cgtctgcttt atgcgactga
    26101 tgcttcgatt tatcagatca tgcccctggg tgtggccttc cccaaatccg cggcccaggc
    26161 cgcgagtctg atgcgggccg ccgctgacgc gggcgtctcc gtcattcctc gcggagccgg
    26221 tacgggcttg gctggtgcaa ccgtcggcga ggggctgatc atcgatttct cccgctacaa
    26281 ccggcgcatt accgaattcg accgcgacaa gtgcgtcgtc cgagtccaac ccggcgtggt
    26341 actcgatcaa ctcaatgcgt tcctcaagcc gcacggactc tggttcggac ccgacgtcgc
    26401 gaccagcgct cgcgcgacgc tcggcgggat gatcgccaac aactcctccg gcgcccacgc
    26461 cccggtctat ggaacgaccg acgatcacgt gctcggtctt gaaatcgtcc tggccgacgg
    26521 aaccctggcg acaatcggcg tcgccggcga cggcctcccc gagcaccgcg aggcagtcga
    26581 tcaaatcatc aatcgctgcg ccaggcaaat cgaggaacgc ttcccgccgg tgttgtgcaa
    26641 gcgatggccg ggctacggtc tcgaccgcta cctgcgcgac aagggcaacc tgacacatct
    26701 gatcagtgcc agcgaaggca cgctcgccct gatcacctcc gcggtgctca aggtcgtgcc
    26761 gatccccggc cgcaagcgga tgggcttggt tttcttcgac tcggtggaag aggccctggc
    26821 cgcgacagtg gaactggtcg acctgaaacc ggcagccatc gagcacatcg accgtctcct
    26881 gttcgaccag accaaagggc aattggcctt taagaccgcc cgcgcactca tgcggctgga
    26941 tgaagacccc tgcgaggcga ttctatacgt cgaattcttc ggcgaggacg gcgacaagct
    27001 ctacgaactg gaaagacgca ggctcggccg gcgcacgctg atctgcaagg acgcggttga
    27061 gcaggagctg gtcgtttcga tccgcaaaca gggactctcg ctgctgagcg cctccaaggg
    27121 ccccgccaaa acgatccccg gaatcgaaga cgcatgcgtg gtaccggcca ggctgccgga
    27181 ttacgtccag ggaatgcgaa agatcttcga cggactcggc ctccgcggat cgttctatgg
    27241 ccatgcggca tccggcctgc tgcacattcg cgccaggatt gacctgcata aggccgagga
    27301 tatcgccaag tatcgaaaga tggccgagga ggtctcagat ctggttcgcg agtttggcgg
    27361 ctcgctggcg gccgagcacg gggtcggctt tgccagggcc gagttcatgc ccgaccagct
    27421 tgggccggac ctcatgcagg ccacgcagga catcaaaaaa ctgttcgacc cgcaaaacct
    27481 cctgaacccc ggcaagatca tcccggacgg ccttcacagg atcgatacgc acctgcgata
    27541 cggggccgga catcggatcg aactgccgtt tgagacggcg ttcggtttca tcgaacggga
    27601 tgagtccttc atcggaaacc tcgaacagtg caacggctgc gggctctgtc gcaaggatgc
    27661 tccgacgatg tgcccgacgt ttctggccac cggcgacgag ttgatggtca cccgcggcag
    27721 ggcaaatatc atccgggcgg ctctcgaaca tcgcacccaa aaccgcgatc cgctggactc
    27781 gccggagctg gaggcggcac tgagcaattg cctctcgtgc aaggcctgca agaccgagtg
    27841 cccggccaac gtggaccttg ctctgctcaa ggcggagatg aatcacgccc gccagcagcg
    27901 agacggcatt cctctggtcg atcgcctgat ctccaacgcc gaactcctgg ggcgtatcaa
    27961 cgccgggctg cttgccccgg ttaccaatac gatcctgcga atgcggctcg tccgatggct
    28021 gatggaaaag actctcggct tcgctgccga ccgcaccatg ccgccgtaca cgcgacatcg
    28081 gttcgacaag tggttcgccc gacgccccaa caagcgcaag ggcgttcgag gcaaggtcgt
    28141 cttgtgggac gactgctggg tgcgctataa cgaacccaac atcggccggg ccgccgtcaa
    28201 ggtgctcgaa acggccgggt tcgacgtcgt tttgcccgac ggccgcaagt gctgcggacg
    28261 cccggcgtgc agccggggcg tgctcaacat ggtcaaggac ctggctcacc acaacatcga
    28321 tctgttcagg acacgcggcg gcaccgaacc gatcatcttt ttagaaccct cgtgctacac
    28381 catgttgatc gacgagtacc gccagttgag gatccccggc gccgaccaag tggccgcacg
    28441 ctgcgtgctc ttcgagcagt tcgtgttcga gctgctgcaa cgccggcccg acgccattcc
    28501 gttcaaggac gcggggctca agatcgccat tcacggtcac tgccacgcca aggccctgac
    28561 cgacgtgacc atcatgccga agctcgcgga aaaaatcccc ggcgccagcg cccgcttgct
    28621 cgacaccggc tgctgcggca tggccggggc cttcggaatg ctcaagaaca aacaggaact
    28681 ctcccgcaag gtcgcccaag acctgctgga aaagatcaac gccacggaac ccggcacgac
    28741 gctcgtagcc tcgggcacca gttgccgcca tcagatcaac cacttcaccg accgtcaccc
    28801 gttgcacatg gccgaggtgc tggcaagcgc catctgacct ccatgctgac gcaggagtac
    28861 tcgccctcca cagcatgcgc tttgcccgtg ttcgcactgc cgatcacgcg gccataccga
    28921 gtcggccgag attcgatcac gcagattagt ttaataaaac ttattgtgct cctgcttcgc
    28981 acgcgttttt gggcgttgca tccgggtctt cacactgcaa caataccacc aagtcatccg
    29041 agttgaagga gatcaccgat gcttcgttcg cctgagtcgc gctgccctct ctatcgcctg
    29101 tccctgacga tcgcaggctg cctgctgctg ggaatcctgg tttctccgac aacggcggcc
    29161 aacgaaaccc cgctgtggag catcgcctgg atcagcgaca cgcagacgcc ggcatgcgag
    29221 tgggtcacag ctctgtgcga gaaggtcaag gccgatcgtc cgagcatggt cattcatacc
    29281 ggcgacgttc ggttcgagtg ggccaaccag tgcgcatgga aggatgtact caacctgctg
    29341 cgcatctcct cgccgcccat cgagtttcat ctcgcccccg gcaatcacga tctgaccaac
    29401 gaggtcctca agaatcatct tcgacgagct gctacgcgcg gaatctatct gctggacacc
    29461 ggcgcgacgg ccgagggaca gggctactac cacagccggt tcacgaccga gatctccggt
    29521 ccgtcgtggc cgatctggaa cccggaggtg gccgttcacc cggcttggca gccggacgcc
    29581 ccccaagccc cttatcgcta cgtcttcaaa cgcggcggca tccgcttcat cgtctgcgac
    29641 tgctactaca ccgatgagca gcgagattgg gtccgtgacc tcatcacgca accggacgac
    29701 agctcggtca ccatcatcct tcagcacaaa catgaggtgg accagttggc caaatacgtg
    29761 gaaggcctcg acggcaagca taacgtcaag ctcatgcttt ccggcgacca ccacaactac
    29821 tgctacgagc agcgccacgg cgtgaccttc atcacctctg caggcatggc cgaaggggcg
    29881 aatagagact ccgatgcgtt cacgctgcgg gtatacaagg atcgcctcaa actcgaccgg
    29941 tatttgctgc ccaagggcaa accggtcacg gcgatcgaag gcccgacaac ggtgtgggaa
    30001 tgcgagggcc gtttctcgga gtatcgccgg ccggagtacc ccgtggcgat tgtcgcctcc
    30061 caacccgcgc cagccgccag cgaggcggcg gtgccgacgc ccacactggc cccgaacctg
    30121 ctgttcaacg gcaacttcga caaccacatc tggtacgagc gctaccgggg ctggtcgccg
    30181 agcggctggt atcagtggtt cacctgcggc ggacatgccc ccgagcacgc agtgggcaac
    30241 gatattccac actcaggcaa ggaatacgtc cgcattcaca tgtgggcgca tgcctggcgc
    30301 ggcggcgtgc tccagaatgt ccgcggggtc gagccgtgcc actggtatcg cctcaccgcc
    30361 tatggctggt tcgccaaaac ggtcgagaat cctgatccca aggcacgcat cggcttggac
    30421 ccgctcggca agctccgaga acagtacggt gtcaacgtga ccagccatcc cgcaccggcg
    30481 tataacgagt gcgtcggaga cgatcccaag acggccggtc ccgactggcc ggacataccc
    30541 gaaagcaccg tttggtccga ctaccacgat tacgacaagt ggggcttctt cgaggtcatg
    30601 gctgaggcga ggtccgacgt cgtcaccgcc atcctctatt gcgacccccg tcagcgaacg
    30661 gccaacgagc ccatctacga gatgaactgg gatacggtgg cgctaaggga aataccatgg
    30721 cccacccggc ggctcgcgcc ggagaacgcc gacatacccg tcgagcaaag catcaagaac
    30781 atgatcctta ccatccagcg gaaactgggc accgcccagt taacctggga cacccccgtg
    30841 ccgtgcggtg catcgcaagt gctgtatcgg tttctggatt ctgaggctgt caatcatctg
    30901 ccggacggcg ggtctccgaa aagaatccgc gtcgaccaat tcccctttga gacaccggtc
    30961 gcctacgagc ggtcggaaaa gtgccatggg atggaaatcg gtccgttgac gatacccgat
    31021 gaagccgagg aactgcaggc cgtcgccctc tcgcgggcgg tcatcaacgg agaatgtcgc
    31081 acgttgtgct cgccgctggc aaggttcaag ctgcggtgag atgttccggg ttttcaagcg
    31141 aactgctgga gcacatgcgt atgaaatcat ttcccgcatc tttgaaagca tgccttgacc
    31201 ggcatccggc cgggccgatc gtactggcac ttgttatcgc atgtgtcttg gcggtaaccg
    31261 tcggctgcaa cacgccggcg acgcggttcg tcgttatctc agacctccac ccggccccgg
    31321 gcgcgttcga tcagcttgag gcgatgaccg accacgtgat ccgacttcga ccggcgttcg
    31381 cggtggtgct gggcgacatc ggcaacgacg aggtgccggg cgtcagcgaa gaggagatcg
    31441 agaccatccg caagtccttc aaccgtatga cagcagccgg cattgaaatc tatcccgtga
    31501 tgggcaacca cgacgtccac catcgcgtgc aggacatcaa agttaactgg ttcctggccc
    31561 agacgccgcg gccgctcaac cacctgttca accaatcggc ccaaacgccg gcttatcaag
    31621 ccttccaggc ccgcggcccc tacaactact cattcaatcg cggcggaatc catttcgcgg
    31681 tcgtggactc gaacatctcc ccgcccagac ccgactggcc cgccgagcgc ctggcccgag
    31741 aacggccgca atgggaagcc catctgcaat ggatgaagga cgacttctgc cgacacgtca
    31801 acaatccacg gcgcctgccg accctcgtat tcctccatca ccccgagtac ctgaccggcg
    31861 atcgtgggat ggattctcgg ccgctgggcc gcgtgctcga ggagtgtcgc gacgaacaga
    31921 ctgtcatggc agcgttcgga ggtcactggc acggcggcgc caacctggct ccgaagtgga
    31981 aacccaacct gcacgtctac gccactcagg cctcggttca ccccgcgacc cggccggtgg
    32041 agttcatcgt ggccgaggtc accgcccacg gcctgacgct ggaacctcgc gactcggtta
    32101 ccggccaacc gggcaccggg ccggtgaagt actatccgat cgccggacgg ttcacagatt
    32161 tgaggcgatg ataagggttc ggggttcagt gaaagggacg ggtgagggca tagggttcgc
    32221 cagacaaaga ctcatccaag ctcgaagctg ataatcatct agcaagcctc cgagaggtat
    32281 gctgttggtt cgtgttcag
//
LOCUS       DAOTPW010000026        31966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1483_length_31966_cov_5.709630, whole genome shotgun sequence.
ACCESSION   DAOTPW010000026 DAOTPW010000000
VERSION     DAOTPW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 31966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31966
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_31966_cov_5.709630"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="P5316_03925"
     CDS             complement(<1..494)
                     /locus_tag="P5316_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84136.1"
                     /translation="MNTMTILLLVAFAAVLCLMSPAALIAGEGEPMDLAEFGQWMIWT
                     GEKLDQPVAVKSLTDAPESAQAVGVQWDDPRDVREIMAEFDGDAPNDVVVEYWFRTWP
                     PPPPSMPNIEDEIDDPWQGKWLPADIKTRIEGRRCVFTFDALGEENPRAENLPGVTYR
                     RTLKV"
     gene            complement(573..1835)
                     /locus_tag="P5316_03930"
     CDS             complement(573..1835)
                     /locus_tag="P5316_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRR84137.1"
                     /translation="MNVGAAQVDITPEPGCELSGFAARTQPSIGVLDRLYAKCLYVTD
                     GTERLLWINVDLIGLLREFVTEFREWAGGKLGLKASQVMISAIHTHSGPATVEISEAG
                     RYDPAYVRFLRARLEEVAQAAVQNTAACVPVFVEGRCDLAVDRRKKASARTDPRVAAF
                     GWRRDDGSFIGAITNYAMHAVALGPSNRMISADLPGRTAAIVSERLPGRPIVLATNGA
                     CGNLNPPYENITSEQLSVWGGQVADAVSGLLPGAAPLTDCTLRLASRVVPIALDVLTV
                     DQIDSIADRYIGHAGSVGEWGDRYTRALEKWRRLRIEEVRSGQCAATADMEIFAVRLG
                     PAVMVGVNAEVFSTFTDLVRQATGRSVYTVGYANGVRGYLPTKAAYDEGGYEVETAHF
                     FYNSFRFKPGGLEHLADEAVQLVRSLFA"
     gene            complement(1832..3244)
                     /locus_tag="P5316_03935"
     CDS             complement(1832..3244)
                     /locus_tag="P5316_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HRR84138.1"
                     /translation="MRPTRHLRLLLVLFSMSFMTAGARSETAVPLLGRGDAGGGKAEQ
                     KHACRPEITKDFTVDLPAAKRPLPEPLPDGRKPGFAIRGTKGWMWTPQQYLAEIPTLV
                     RAKMNFLMNCYLSMCDIEHHPWGDKRCNRWWEPLPEAKKEAYERIVRECQKAGIDFCF
                     SMNPNLGTERILDYGKAEDIDLLWNHYRWMQGLGVKWFNIALDDISEGIDASGQARVA
                     NEFLKRLRAGDPHAQLIVCPTWYWGDGTDARHRPYLETFARELDKDIYLFWTGDAVVI
                     NRITRKCAETYKSIVKHRLILWDNYPVNDNNPTMHLGPVFGRDPDLCEVIDGYMSNPL
                     CKQNELNRIPLLTCADFAYNPWAYDPARSIGQAILHFADDPAQQVLVKDLVEAYPGML
                     YYGKGTGFNAVREQYARIAAAPHSRYVARMYIDYLHSLAGRMEKAFPDRLKAERQTVI
                     DDITWMNRAFAEKYGPEQAE"
     gene            complement(3304..4599)
                     /locus_tag="P5316_03940"
     CDS             complement(3304..4599)
                     /locus_tag="P5316_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRR84139.1"
                     /translation="MTKESPKLKDERIQWWLEARFGMFIHWGTYSLAARHEWVKNYER
                     IPTDAYQKYFDHFDPDLYDPQEWARAAKGAGMRYFVITTKHHEGFCLWDSKFTDYKVT
                     NTPYGKDLIKPMVEAFRAEGLRVGFYYSLLDWHHPDYTVDSCHPMRDNKEEREKNKNR
                     DMKRYVDYVENQLRELLTQFGPIDCLFADFSFPGPDGKGRNDWQSERLLKVIRELQPD
                     CLINDRLDLLDRPDGWDFRTPEQFMPRSWVSMDGMRVPWETCQTFSGSWGYHRDEATW
                     KSPHQLIVMLIETVSKGGNLLLNVGPTARGTFDDRAMECLAAIGDWMWLHSRSIYGCT
                     AAPDNIKKPDNCLLTYNPKTRRLYVHVLVWPMGKLQFDGMSDKVAYAQLLNDASEVRF
                     STNKGPWMAEQQDAADGITFDLPVRQPNVAVPVIELYLK"
     gene            4982..5536
                     /locus_tag="P5316_03945"
     CDS             4982..5536
                     /locus_tag="P5316_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRR84140.1"
                     /translation="MPSALSQIRPDNALLLVVDLQDRLLPAIHDSTAVVEACRRIIEA
                     AKVLEVPMVVTEQYPEGIGRTCQPLLECLGDAPLYTKIRFSACVESVVEKLASLRRPN
                     VIVIGIEAHVCVMQTTLDLLRLGYTPYLCADAVGSRRPHDRDMAIERVRQAGAIVTTT
                     ESVTFELMGQAGTEVFRQILKIVK"
     gene            5942..6268
                     /locus_tag="P5316_03950"
     CDS             5942..6268
                     /locus_tag="P5316_03950"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRR84141.1"
                     /translation="MSAGSRKDSPGDQRRWMPIRREGLCCRTFDDEAVLYDPAHHAVH
                     YLNRTAWFIWNCCNGHRDVEDITLEVAAGFEPNAQQDNPLAAIRDDVRATLSRLTENG
                     LIESAP"
     gene            6265..7221
                     /locus_tag="P5316_03955"
     CDS             6265..7221
                     /locus_tag="P5316_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84142.1"
                     /translation="MTRLAPPNVTDVSDSQRSRVGFCLFGPKPEVRHYRIGPVPVSLA
                     SILTGVATDFHELYRAYSIEEPTGNEFAIEVIVKRSARSLRRYYHVVANGERLFAMRS
                     ENEVLPHVEWAINTMVATRLSEYLQIHASVVARDGVAIICPGQPGQGKTTLAAALLSR
                     GWSYLSDEFALLDPETRLLEPYPKALCIKAGSFEVLRRLRLPIDTDRVYYKGEKGQVT
                     LLNPLRIRPDVVADRCRVGMVVFPEINRSTTPVIERVSRAQTVFELTRCAFNFGRFRA
                     TGFNLLADVVERAGCYRLRCGHLATTCDLVEAFFTREVGSGV"
     gene            7301..8398
                     /locus_tag="P5316_03960"
     CDS             7301..8398
                     /locus_tag="P5316_03960"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRR84143.1"
                     /translation="MAVEGLWRDGLWSQVVDHACSSGVGGAVARNMAAREVEIPRSAA
                     IQLDSYRQHVAAAAAYGLEKTEPILACLQKAGIAFLVLKGAALNAALYDGLGLRPMMD
                     VDLLIRPSDAQRADAALTSLGCRPGPDLVNEDFYPRFYYEREYFLPCRPEVKIDLHVR
                     PFRPLRYAVTLPDHAMWDEPMPVRYGRLTVQIPNVENMLIHLAVHAACHGAAELKWLY
                     DLKLWLDRYGDRLDIRRLVDKCQRWKIGWPVRVVLEHVDSLFGEGSVYLERTLSALKG
                     CRAGWRDRLALVQSPRDAAHPLAAVAVNVLCTPGVRFKLGYLRAVLLPARSHLAQLYH
                     GRHPGWPLVAHAVRIGRRLTRRFCRTRPASV"
     gene            8494..10401
                     /locus_tag="P5316_03965"
     CDS             8494..10401
                     /locus_tag="P5316_03965"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRR84144.1"
                     /translation="MESRITFVAAAAVFMASMAVAGEMGIAKMKAVAPTQVKLTDSFW
                     APRLEINRTVTLPHNIKMCEETGRISNFDKAAGKMQGKYEGAYFNDSDVYKVLEGAAY
                     ILAAHPDKEMDARVVDIIARIAAAQQPDGYLNAYYTVMEPDKRWTDLPKMHELYCAGH
                     LTEAAVAWADATGRTDFLDVARRFIHHIEGIFGFDKRHGVPGHEEIELALVKLYRKTG
                     EERYLKLAEYFLAARGTKPEKGAEYRQDHIPIKQQKEIVGHAVRAMYLYSGVTDVAAI
                     TGDKEYLQTLDTIWHDVVTRKMYITGGVGARHEGEAFGGAYELPNDSAYAETCAGIGL
                     AFWAHRMLMLHGESRFADVLERELYNAFPSSVSLKGDAFFYVNPLSSKGEHHRQPWYG
                     CACCPTNIVRVMPQVPGYAYSQSDDGVWVNLYMAGEGTVALKSGEVKLAVDTKYPWEG
                     KVKIKVTPREPAEFAINLRVPDWCEQVTVSLNGTAIPNPTMDKGYAVIRRQWKAGDAI
                     EMDMAMPVRRIQAHPNVAANRGRVALQRGPMVFCLEAVDNGGKVFDLVLPRDAELKTE
                     FRPNMLGGIMTIKAQGLRCPARDWKDKLYLAAPAHAEPVELTAVPYCIWDNREAGEMT
                     VWIPEITGLSN"
     gene            complement(10478..10942)
                     /locus_tag="P5316_03970"
     CDS             complement(10478..10942)
                     /locus_tag="P5316_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HRR84145.1"
                     /translation="MSDTNAVKWLRSQGVPFEVLEYTFTEVGADRAAEAVGRPLESCC
                     KTLIVKAVGKAFWVAIVPGDQRFDTRKMAGAIGAAEAELAEHDEAEKITGYRVGGISP
                     FAMRRQLPVVIEESLLALDRIIVNGGRRGVLVEMATEDVVRLLNARPADICA"
     gene            complement(11000..11569)
                     /gene="cyaB"
                     /locus_tag="P5316_03975"
     CDS             complement(11000..11569)
                     /gene="cyaB"
                     /locus_tag="P5316_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRR84146.1"
                     /translation="MPVEVEAKIKVDSHEAVLERLSSLRAMRICRVLETNHIFDNRDR
                     TLLSSGRGLRVRICRAEDGNDPPATLTYKGPRIQGPLKTREEIEIATDDGEACKELIQ
                     ALGFVEVLCFEKRRETWRLGDCTIELDEIPYLGRYIEIEGPDEAAVVQAQRALGFGEC
                     RTVRQSYVSLLVEHCRRNGLAIDRIAFAS"
     gene            complement(11654..12304)
                     /locus_tag="P5316_03980"
     CDS             complement(11654..12304)
                     /locus_tag="P5316_03980"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRR84147.1"
                     /translation="MLTLDAKHIRELREDLQHLAPPYQLRKYEATIEDYEEPAHEDLR
                     CEYLDEVQIVHSPASVQHEERLIFLATLLNTVVAGRRLGRMYASNAVMQLGERRFCPD
                     LSYLAAEHTDRIRGTRVVGPTDLVVEMAFTDTREYDLGEKHAACHQGRIPKIWFINAD
                     HKQVQVNVLEGEVHLTNTFTNGRFDGRILPGISIEVNWFWTEPLPNPLECLKTATK"
     gene            complement(12898..13371)
                     /gene="ilvN"
                     /locus_tag="P5316_03985"
                     /pseudo
     CDS             complement(12898..13371)
                     /gene="ilvN"
                     /locus_tag="P5316_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(13425..14171)
                     /locus_tag="P5316_03990"
     CDS             complement(13425..14171)
                     /locus_tag="P5316_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRR84148.1"
                     /translation="MIRTLLRTRIVAGLLTLIPIWVTWVVVKFVFDNLRSAMDPLAGR
                     VAKALLEHNKALLPQTVNDYQEWVVPAVTVLLTLFLLYLLGLFSANVFGARFLLWLDG
                     IFGKVPLVKTIYRSTKQIVLTVGGGQSSYFQRVVLVEYPRPGMKCIGFLTSVVDDADT
                     GRKIAAIFIASTPTCSTGYTQLVPLEEVSETDWTVEEAVKAVMSGGLVLPENVRFDRV
                     HPVKMIVPQARAAEMGGVPLQPASQVQRGA"
     gene            complement(14267..15925)
                     /gene="ilvB"
                     /locus_tag="P5316_03995"
     CDS             complement(14267..15925)
                     /gene="ilvB"
                     /locus_tag="P5316_03995"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRR84149.1"
                     /translation="MVHDTLVDLGVEVAFGYSGGAILPTFDTLHDTPIRFIVSRHEQG
                     AGHMADGYARATGKVGTVIVTSGPGATNLTTALATAYMDSIPLVAITGQVATAAIGND
                     AFQEADVVGVTRPVTKHNVLVKDVNNLERIIREAYFIARTGRPGPVLIDLPKDVQVSI
                     PDDGNNREPLLPGYRPRCKGNQRQIRLAAQAINASERPVLYVGGGVISSNASAELRKL
                     ARAGNIPVTTTLLGLGAFDEVADSDLALHMLGMHGSAYANYAVQASDLLIAVGARFDD
                     RVTGKLDTFAPHARIIHIDIDPSSISKSVDVDIPVVGDAREVLCEMLPLIERRERAEW
                     FAQINEWKRRFPFSYQPQAGVIKPQAVVEEICRQTNSEAVIATGVGQHQMWAAQFYRW
                     RFPRQMISSGGLGTMGYGFPAAIGAAIGLSDKIVIDIDGDGSFLMTCSELATVAERKI
                     PVKVVILNNAFQGMVRQWQELFFGRRYVGTQMVNPNFARVADAFGCTGIEVKDPQDLP
                     KAIKKMLDTPGPVVVDAHVAREENVYPMVAVGKSLHEMEMGGMS"
     gene            16412..17782
                     /locus_tag="P5316_04000"
     CDS             16412..17782
                     /locus_tag="P5316_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR84150.1"
                     /translation="MWRFAPAVVICAIVAGSSQGAPRNVVLMIGDDHGMQVGCYGDKV
                     IKTPSMDRLASQGTRFANAFAAVSSCSPSRAVILSGMFTHSNGQYGLAHATHNFHSFH
                     DVQSLPRQLKEAGYRTAIIGKYHVQPEETYPFTETLPCPGGPRNVAAMAEQARRFMGA
                     QGDSPFFLLVGYTDPHRDAKGFANRPKYDGIETITYKPEDMIVPPWLSDHPEVRKELA
                     EYYQSISRLDQGVGLMLKAIADTGHENDTLTIYVSDNGPPWPGAKTTLYDPGIHLPLI
                     IRSPQQKKAGVVNEAMVSFVDLTPTILEWAGVEKPSKVFGRSLLPILEETRPEGWDTV
                     YGSHIFHEVTMYYPARMIRTRQYKYILNLAHRLEFPFASDLWGSDTWQGVLKRGDNQY
                     GKRTVDAYIHRPREELYDLQADPHETKNLAGDPAHAQTVADLRARLLEWQKKTEDPWL
                     VKYQHE"
     gene            complement(17869..19215)
                     /locus_tag="P5316_04005"
     CDS             complement(17869..19215)
                     /locus_tag="P5316_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR84151.1"
                     /translation="MIQTAQEPLSKAPPIAPTRTRFWVIVFAVTLAVVTYIDRVCISQ
                     AAPFIRQDLGLSEVQMGLAFAVFFWTYALFEIPGGWMGDRWGARRVLMRVVVWWSFFT
                     VATGWAWNLASLVTARALFGAGEAGCFPNVTKAFATWLPRGERVRAQGLMWLSARWGG
                     AFTPLLVILVLKFLSWRQAFGAFGLLGVIWAVFFYRWYRDDPRDNPHVNAAELALIPT
                     ASETAPVHQAVPWRKLLRSKTVWLLCAQYAFLSYGWTFYITWLPTYLKEARGLQLGTG
                     AVLAGIPLLFGGVGSLCSGFIGGHFERKGGNARRIRRLMACAGCLGAAGCTAVSVSIH
                     SPLTAMIVMGLASFFMDLAVPPSWAVCMDVGGRACGTLSGGMNMMGNLAGSLGPLATG
                     FLLVRTGHNWDVVLYTFAGAYFMGTFCWMFLDPVTPLLPATPVRGSEVVAVRETGN"
     gene            complement(19413..19847)
                     /locus_tag="P5316_04010"
     CDS             complement(19413..19847)
                     /locus_tag="P5316_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84152.1"
                     /translation="MKRLACLALVGLVLPLVGACDLMKLLQGKATTVQLVNNGDYPVI
                     VELRISNNQYVLQELLREFGTELTYTIPPGQAVTFFKDCEDLQAILIKKADLSLVGTV
                     GPSASTDILRDGGDFGCGDIIIYTFDHPDVPTALEISENVIG"
     gene            20422..23691
                     /locus_tag="P5316_04015"
     CDS             20422..23691
                     /locus_tag="P5316_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84153.1"
                     /translation="MRPKSVLTTVILSSSMVTVFAKADLTYNYEILSAGVEAGGFVLP
                     DETLTFVEQVYDDTPEPYNHQAELITNTLTGRGLSILDVSIAPDSYDFQHIDAALLDL
                     QAELTETGYSKVFAQGDSYFQFWVQPRDRNYEYVVRVEQEYDDGLVPAYLAGGDRAFD
                     LFAVLEGVGNTTPGEAGFWYFVGTLAPGVDYHWYGGVRAAAESNGLLSGPFNARLRMD
                     FTLVPETDDYLWIGPDRGLFAESSFWDPSGTPWENGRAIFNSPSGADQTVLFDDDHRT
                     GRLVVTAPAGQNTDDQIVLDLDGWNYWILREWNDVYRASLTVGNTANATANLAIVDGH
                     VDAKDVEIGLNGTGQLTIGEDGVFTVVAHGVSVGTQDYGSLHIVDGGWMHHGHGFVGQ
                     FAGSHGTITVDGTKPSDPNVRSYWQAGGWLGLGDQGEGSLYVANGADAETGKCDMACN
                     PGSTGMADISGDGSQWELQSHWEVSLIVGKSGSASVTTWDGGSVVNRGEMHLAETPGS
                     TGTIVLVGPGRDNSGATIPALECEQRLTVGYGGVGDFELWADAWAVVHGPVIVGTDPN
                     AEESSYSQVIVDGADSLLEVSGKDGAGSYIGMEIGRQGAGNLLSITNGGQVTTGYTTT
                     IGGGGGDATLLVDGLDSLLLSHFQFEVGRDGPGEVTVSNGGRIYVDGSNTDPEWDDGF
                     KGLTHIGMRSTGILTVTGMDGGTPSTLESMAQISLGSNTGGNGTLNIEDGGLVVSWKH
                     VSPTESAGIVGRGAGGTGLVTIKDGSTWQMPDGGLTVGWMGKGRIEMAGGYMFSTYGV
                     IGRTPGSQGEVELSNPTGSDPSHWDIDGPLAIGGDLAGTVGGTGRLFIGRDCWVWTSG
                     GTTIYTSGTLSLAEGGIDTDITLHGTIEGVGWIARPVTNVSGVVHPTYTGTGALRELT
                     FRQGYVQQSGGTFLVDVAGDGDSSVIVTWDSAYSISLAGKLKVKILNGYIPAINSRFL
                     IAHAEGGIINEAFDTLDLPTIDGQPVFELYAEGTSLLWLRTLRDYHAPVDYDGDGDVD
                     QDDLDIFEACATGPGIPFADGNCAKADLDRDTDVDQTDFSTFQRCYSGENVPVNADCA
                     N"
     gene            complement(23765..25165)
                     /locus_tag="P5316_04020"
     CDS             complement(23765..25165)
                     /locus_tag="P5316_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR84154.1"
                     /translation="MGRKQRLVTMVVSALAAGSQATAAPGTRTNLVLIMTDDQGAWSV
                     GCYGNSEARTPVLDKMAAGGVRMTRAFATTPVCSPSRATFFTGRIPSQHGIHDWIKHE
                     NMGPRARYCLPREKQISEVLAEAGYVCGLSGKWHLGDSLHARAGYTFWYAMPQGGSQY
                     NDAEMIWQGELVKAEGYITDRITDKAVEFLRTNRDRPFFLNIQYNAPHSPYTGQPKEL
                     VDLFADVPFASIPTLAPHPWAASGLTSLHNQREPLEQYFAACSGVDRAVGRLLAELEM
                     LGLIRDTLVVYTSDQGFCTGHHGFWGKGNGTNPRNAYDTSLRVPMIFSHPGRLPAGAT
                     VDAMVSAYDFMPTVLDYLDVKTPTDRNLPGGSFAPMLRGEKQPDWPDAVLGEYGQLRY
                     IRTAAHKYIHRANGGPFELYDLSRDPQETTNLVDLPEHRELRTVLRKRMLDWFEQYVE
                     AGADPVGQEYIRPDER"
     gene            25316..26380
                     /locus_tag="P5316_04025"
     CDS             25316..26380
                     /locus_tag="P5316_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRR84155.1"
                     /translation="MRIAFVGPPLSGKSTLFRAVTGQAAPSHPGLGEHLAVVKVPDPR
                     LHWLYNHYKPKRMVEATIDCLDVPGFSHETAQQQAEFRKSLPHLRQADALVAVVRAFD
                     SPMVPPYRDRVDARADLEELVAELIFCDLEAVMNRIEKIEKALTKPTKTHEPEKRELA
                     LMKRCQEALENEKPISSIIETDEERKTLSGYQFLTELPLIAVINVNEDQAAKPAPFEW
                     PQAKATIALCAETEAEIAELPPEDRKAFLEDLGVSESAKDRLIKTCYEAVGLISFLTV
                     GDDEVRAWSIKRGSDAVEAAGKIHTDIARGFIRAETVAYNDLHAAGDMKAAKAAGKVR
                     LEGKTYIVQDGDVINFRFNV"
     gene            complement(26370..27191)
                     /gene="trpC"
                     /locus_tag="P5316_04030"
     CDS             complement(26370..27191)
                     /gene="trpC"
                     /locus_tag="P5316_04030"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRR84156.1"
                     /translation="MGNILDQIVETKRGEVATAKRRRPLSELKVALRDTRPTRDFIRA
                     VAKSPARDVNLIAEIKRESPSAGLIRDDFDPVDLARVYHSVGADALSVLTDRTYFGGS
                     LEFIARVKETVPIPVLRKDFIIDEYQLYETRVAGADAVLLIGEILPPARLADLLELAY
                     ELGLASLIEVHEPETLEQLQSVVGFPNRKRGLLGINNRNLKSQQTDLATTEMLAKQVG
                     KDVVFVSESGIKTRADVQRLTQAGARALLIGETFMRSPDVAAKVLELLGPVPVTR"
     gene            27293..28303
                     /locus_tag="P5316_04035"
     CDS             27293..28303
                     /locus_tag="P5316_04035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRR84157.1"
                     /translation="MMAVLAVLSANTMQTIDLSLLVAGGVVIVALAAKWWQSGRRDPL
                     CGSPIRLNRLTPVWLWFCVLAQLLAWMIAGQAASWMDGTSAETLAGSASRPVAPVSSG
                     ESDVLPPDSQPIPRALTSSPATAATESNPSPKPRSTVLAANLAQVLMIAACLVLARMT
                     FPRGWRQFGFGRRPLSKDMPWALAAWLAALLICTMLYWGSIGLWRLLWPDSVPPEHTV
                     LTVVKDAEAGLGVRIIAIVGAVILAPVGEEIFFRGLLQSGVKKLAFVRRGSWQHRWMA
                     IGVAAGLFGLMHTVTPQHIPALIALGMILGYAYERSGSLTLPILVHMLFNAKTLLWVA
                     LG"
     gene            complement(28327..30420)
                     /locus_tag="P5316_04040"
     CDS             complement(28327..30420)
                     /locus_tag="P5316_04040"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRR84158.1"
                     /translation="MNRNLVLIWTVMQCFMLLACSQRAEGADKTQASKGLPFDIGTLD
                     ASAIKAAGPRLFFTQDEIERATARLDTDEQARAILDNLKAEADKHLQIEIKPLDESWW
                     NKAKSKKWEETYPEVFENTAIRPGAYAHPAATLAVAWLLTGKQEYADKAVALLKNLTP
                     YSFAPEHFDVGMNYAGWGLSMLKAYDALLPALPPSQRKAIDACLTRMAKAVAKNDVYW
                     IENDVGGGINNHLAWHKMMLGLLGMFYNRPEMVDYCLHGRRGLVSLLEDGLTDDGLWC
                     ESSLNYQFAAIAPMLIVADCQRRLGISPGLHGLVAGNGRMLKQSFDAMFNVLSADGMI
                     PPVGDAYGHHQRLYDNKLYETCWLLWGDSKYAWLISHSENPSVTMLFAPPLPQNVPSP
                     PIGSILLPEHGYAFLRSKQDDLYWDTDAWCAFLTYDRSGVHANADKLSLTLFGQKRML
                     VSDVEGRATVPHAFSSKIQSELNRGALSQNTVMIDGGDQRCSSEMLRLIEFRDLPEEK
                     RVTAADDRGILYEGIRQMRTIAMTPEYVLDVFQVDCGAKPRQIDWVAHILDGHARMSE
                     SGNPDLASREPFELPKGAAWKWLRDPQSFKPTGPIRMEWQDGEVRLRLRMLDPGIERV
                     ILCGYPSTDQPDSPSIPMIMVRKKEKQAIFAAVWLIGSEIEEVEFKTLPRHNGMLVYQ
                     VKAGETTRKHLVPAL"
     gene            complement(30734..31084)
                     /locus_tag="P5316_04045"
     CDS             complement(30734..31084)
                     /locus_tag="P5316_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84159.1"
                     /translation="MTRIAQSTLFVALYLFIGAMLLVACGRDTNSKAGDKSQAVNVIC
                     TRCGADATIQITGDVKDEVWPKPCPSCKRSSAYPSGKCSQCGKLIPLMDPRTRDYAVP
                     QSCSHCGKPWEPQS"
     gene            complement(31129..>31966)
                     /locus_tag="P5316_04050"
     CDS             complement(31129..>31966)
                     /locus_tag="P5316_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84160.1"
                     /translation="VAILALLVAILLPSLQGARNQAKLTHCKASAKQIATAMSIYQAE
                     AQGYVPIMLNWHSGPVYNAPARAVFLSVALRSVEKGLFGLSKRVSTTGQHFDPNESWS
                     SDTRDDYEARFLPAHYVCPFERGRQPWDLRHVGFAPPHTLWEWNGVMESYQTWLWEDI
                     VRDQQVYSEPVGWSRPTDGLPQYSVMTWNQVRQTGKPPSDPAIKNLLHRRWTDGEARL
                     QKAAGLGGVTVIYCAIGEHMEMGSRRIDMGSHRTSTGGGTNAIFGDTHVEWVRGTRIG
                     WP"
BASE COUNT         6151 a         9789 c         9912 g         6114 t
ORIGIN      
        1 accttgagcg tccgccggta ggtcaccccc ggcaggtttt ctgcccgcgg gttttcttcg
       61 cccagagcgt caaacgtgaa aacacaccgc ctgccctcga tccgcgtctt gatgtccgcc
      121 ggtagccact tgccttgcca cgggtcgtcg atctcatctt cgatattggg catgctcggc
      181 ggaggcggcg gccatgtgcg aaaccagtat tcgacgacga catcgttcgg cgcgtcgccg
      241 tcaaactccg ccatgatctc ccgcacgtcg cgagggtcgt cccactgcac gccgaccgcc
      301 tgtgcgctct ccggggcgtc cgtcagggat ttcacagcga ccggctgatc aagcttctca
      361 ccggtccaaa tcatccattg gccgaactcg gcgaggtcca ttggctcacc ctcgccggcg
      421 ataagggccg ctggtgacat gaggcataga accgccgcga aggcaaccag tagaagaata
      481 gtcatggtgt tcatggtagg acctttctca gaagctgccg gccgagaacg cgatcgtcaa
      541 caaggctgtc gaacctcagc acccaggctc gctcaggcaa acaacgaacg caccagttga
      601 acagcttcat cggccaggtg ttccagacca ccgggcttga agcggaagct attatagaag
      661 aagtgagcag tctcgacttc ataaccgcct tcatcgtagg cggccttcgt cggcaggtac
      721 ccgcgcacgc cgtttgcgta gccgaccgtg tacacgctgc gtccggtggc ctggcggacg
      781 aggtcggtga acgtactgaa aacctccgcg ttcacgccaa ccatcactgc cgggccgagt
      841 ctcacagcga agatttccat gtccgcggtg gcggcgcact gcccgcttcg tacctcctcg
      901 attcgcagtc gacgccactt ctcaagcgcg cgcgtatagc ggtcacccca ctcgccgaca
      961 ctgccggcat ggccgatgta gcggtcggct atgctgtcga tctgatcaac agtcaacacg
     1021 tccaatgcga tcggcaccac gcgcgaggcc agcctcaggg tgcaatcggt cagcggtgca
     1081 gccccgggca acaaacccga cacggcgtcg gccacctgtc cgccccatac cgacagttgt
     1141 tcggaagtga tgttctcgta cggcgggttg aggttgccgc aggccccgtt ggtggccagc
     1201 acgatcggac ggccgggcag ccgttcggac acgatcgcgg ccgtgcggcc cggcaggtcg
     1261 gcactgatca tgcggttgct cggccccagt gccacggcgt gcatcgcgta gttggtgatt
     1321 gcaccgatga aactcccgtc atcgcgccgc cagccgaacg ccgccacgcg aggatcggtc
     1381 cgggccgaag cttttttgcg ccgatcgacg gccaggtcgc atcggccctc aacgaagacc
     1441 ggcacacagg cggccgtgtt ttgaaccgcc gcttgtgcta cttcttcgag cctggctcgc
     1501 aggaatcgca cgtatgccgg gtcgtaccgc cccgcttcgg aaatctcgac cgttgccggc
     1561 ccggaatgcg tgtggatcgc cgagatcatc acctgagaag ctttcagacc cagctttcca
     1621 cccgcccact cgcggaactc ggtgacgaat tcccgcagta ggccgatcag atccacgttg
     1681 atccaaagca gtcgctcggt gccatccgtc acgtacagac acttggcgta cagtcggtcg
     1741 agcacgccga tcgatggctg cgtgcgggcc gcgaaaccgg acagttcgca ccccggctcg
     1801 ggcgtgatgt cgacttgggc ggcaccgaca ttcattcggc ttgctccggc ccgtacttct
     1861 cggcaaacgc gcggttcatc caggtgatat catcgatgac cgtctgccgt tcggccttga
     1921 gccggtcggg gaaggctttc tccatccggc cggccagcga gtgcagatag tcgatgtaca
     1981 tgcgcgcgac atatcgggaa tgcggggcgg cggcgatacg ggcatactgc tcgcgaaccg
     2041 cgttgaagcc cgtgcccttg ccgtagtaga gcattcccgg gtacgcctcg accagatcct
     2101 tgaccaggac ctgttgcgcc gggtcatcgg cgaaatgcag gatggcctga ccgatcgagc
     2161 gcgcgggatc ataggcccag gggttgtagg cgaagtcggc gcaggtcaac agggggatac
     2221 gattaagctc gttctgcttg cacaacgggt tgctcatgta gccgtcgatg acctcgcaaa
     2281 ggtccggatc acgcccgaag accggcccca ggtgcatggt cggattgttg tcgttcaccg
     2341 gataattatc ccacagaatc aggcggtgct taacgatgct cttgtaggtc tccgcacact
     2401 tgcgggtaat gcggttgatc accaccgcgt caccggtcca gaacaagtag atatccttgt
     2461 ccagctcgcg ggcgaacgtc tccagatagg ggcggtgcct ggcgtctgtg ccgtcgcccc
     2521 agtaccaggt cggacacaca atcaactggg catgcgggtc gccggcgcgc agccgtttca
     2581 gaaactcgtt ggccacccgc gcctgtccgg aggcatcaat gccctcggaa atatcatcca
     2641 aggcgatgtt gaaccacttg acgcccagcc cctgcatcca acgatagtgg ttccacaaga
     2701 ggtcaatgtc ctcggccttg ccgtagtcga gaatgcgctc ggtccccaga ttggggttca
     2761 tgctgaaaca gaaatcgatg ccggccttct ggcactcgcg gacgatccgc tcgtatgctt
     2821 ctttcttcgc ttccggcagc ggctcccacc aacggttgca gcgcttgtct ccccacgggt
     2881 gatgctcgat gtcacacatg ctgaggtagc agttcatcag aaagttcatt ttggcgcgga
     2941 ccaacgtcgg gatttcggcc aagtactgct gcggtgtcca catccagcct ttggtgccgc
     3001 ggatcgcgaa gcccggcttg cggccatcgg gcagcggctc gggcagcggg cgcttcgcgg
     3061 ccggcaagtc gacggtgaaa tccttggtga tctccggacg acaggcgtgc ttctgctcgg
     3121 ccttaccacc gccggcatcg cccctgccaa ggagcgggac ggcggtctcc gatcgagcac
     3181 cggcggtcat gaatgacatg gaaaacagaa ccaagagcaa acgcagatgg cgggtcggtc
     3241 gcatcctatt ctgactccgt acaaagagac tccctggctt gtgcttgcct cgatcacacg
     3301 cgttcacttc agatacaact cgatcaccgg cacggcgaca ttcggctggc ggacgggcag
     3361 gtcgaaggtg attccgtcag ccgcgtcctg ttgttcggcc atccacggac ccttattggt
     3421 gctgaatcgc acctcgctgg catcgttgag cagttgggca taagccacct tgtcggacat
     3481 gccgtcgaac tgaagcttgc ccatcggcca gacaagcacg tgaacgtaga gccgcctggt
     3541 tttagggtta taagtcagca ggcagttgtc aggcttcttg atattgtccg gagcggccgt
     3601 gcagccgtag atggaccggc tgtgaagcca catccagtcg cctatggcgg ccaggcattc
     3661 cattgcccga tcatcaaacg ttccccgggc ggtggggccg acgttgagca gcaggttgcc
     3721 gcccttgctg actgtctcga tgagcatgac gatgagctgg tgcggactct tccaggtagc
     3781 ctcgtcgcgg tgatagcccc acgagccgct gaaggtctga caggtctccc agggcacgcg
     3841 cattccgtcc atgctgaccc aactgcgcgg catgaactgc tcgggtgttc ggaagtccca
     3901 gccgtcagga cggtcgagaa gatccagccg gtcgttgatc agacagtcgg gctgcaactc
     3961 tcggataacc ttgagcagtc gctcggattg ccagtcgttt cggcccttgc cgtcaggacc
     4021 ggggaaggaa aagtcggcaa agagacagtc gatcggcccg aactgggtga gcaattcccg
     4081 caactggttc tcgacgtagt ccacgtatcg cttcatgtcc cgattcttgt tcttttcgcg
     4141 ctcctccttg ttgtcacgca tcgggtggca actgtcgacc gtatagtcgg ggtgatgcca
     4201 atcgagcagc gagtagtaaa aaccgactcg cagcccctcg gcccggaagg cctcgaccat
     4261 cggcttgatc aaatccttgc catagggcgt gttggtcacc ttgtagtcgg tgaacttgga
     4321 atcccacagg cagaagccct cgtggtgctt ggtcgtgatg acaaagtagc gcatgcccgc
     4381 gcccttggcc gcccgggccc attcctgcgg gtcatagagg tcgggatcga agtggtcgaa
     4441 atacttctgg tacgcgtcgg tcgggattcg ctcgtagttt ttcacccact cgtggcgggc
     4501 ggccagtgag taagtgcccc aatggataaa catgccaaac cgggcctcaa gccaccattg
     4561 tatccgctcg tccttgagct tgggcgattc cttggtcatt tcagccccct gtacaaacag
     4621 cgggaagagc aagcagaccg tcagacaaca acattgatac gctttcatga gtcgaccctt
     4681 tcgataatca cgtgtatctg attgggtcag aggctatccc aacagattcg ggctgtccag
     4741 tgggtgagga gagacccggc tgcgcctcgt gcgacaaggt atgctccctg gcgcgcacga
     4801 aagtggacgt tctcggcagg tctcgacggc ggtggcgatt tcgactttcc tgccggcggt
     4861 gatcgtcgac acatgtgcaa ccattgaagg ggtgcgcccg cctcgtgcga cgctcaactg
     4921 actcgttgac tgtgcttcgc cgggcaccta cagtgttgcc gacaaacgga ggaaacgaac
     4981 cgtgcccagt gctctcagcc agatacggcc ggacaatgcg ctattgctgg tggttgacct
     5041 gcaagaccgc ctgctgccgg cgattcatga cagcaccgcc gtggtcgagg cctgccgccg
     5101 catcatcgag gccgccaagg tgcttgaggt gccgatggtg gtgacggagc agtatccgga
     5161 gggcatcggc cgcacttgcc aaccgctcct cgaatgcctt ggcgatgcgc cgctttacac
     5221 caagatacgc ttcagtgctt gtgtcgagtc cgtggtcgag aaactggcct ccctcaggcg
     5281 ccccaacgtg attgtcatcg gcatcgaagc ccatgtatgc gtcatgcaga ccaccctgga
     5341 cctcttgcga ctcggctaca ccccctacct ctgcgccgac gcggtcggat cgcggcggcc
     5401 ccatgaccgc gacatggcca tcgagcgagt tcgccaggcg ggggccattg tcaccacgac
     5461 cgaaagcgtc acgttcgaac tcatgggcca agccggcacc gaagtcttca gacagatcct
     5521 caagatcgtg aagtagtcgg gcagtccgat aacagagcgg ccggccgttc tgctgaccgg
     5581 ttttgggccc tcgtcttttg actcgaaacc cggcgaagaa tggccgataa ccatggtgga
     5641 gggcggcggt ggactgcgcg agggttgtca gaagaatggg agacttctgg gtggataatc
     5701 gtcgactgga aagcagtccc cttgcggagg aacgggagga gccgcaggcc ggtgtgatcg
     5761 catcagaggc tcctgcttcc caagagctgg actcaagcgg gacgcggcga cgcttcctca
     5821 gttgttcggt gcgcaaattg gcctatgtat ctccggtagt gttgctgttg cggccgcgtc
     5881 cggcgtgcgc gtcgcggatc tactcgtgaa gtgattggcg cggctaatgg atcccatgac
     5941 gatgtcggca ggttcacgga aggactcccc gggcgaccaa cggcgctgga tgcctatccg
     6001 gcgcgagggc ctttgttgtc gtacgttcga tgacgaggcc gttctctacg atcctgcaca
     6061 ccatgcggtc cactatctta atcgtacggc ctggttcatc tggaattgct gcaacggcca
     6121 tcgagacgtt gaagacatta ctctggaagt tgcggcgggg ttcgagccga acgcccaaca
     6181 ggacaatccg ttggccgcga ttcgcgacga tgttcgggcc accctgtcgc gcctgaccga
     6241 gaacggattg atcgagtcgg ccccatgacc cggcttgcgc ctcccaacgt gaccgatgta
     6301 tcggattcgc agcgatcgcg cgtcggcttc tgcctgttcg gccccaagcc cgaggtccgc
     6361 cattatcgaa tcgggccggt gcccgtgtct ctggccagca tcctgacggg cgttgcgact
     6421 gattttcacg agttgtatcg tgcttactcg atcgaagagc cgaccggcaa cgagttcgcc
     6481 atcgaggtga ttgtcaagcg gtccgcacga tcgcttcggc gttactatca cgtggttgcc
     6541 aacggcgaga ggctgttcgc gatgcgcagc gagaacgagg ttctcccgca tgtggaatgg
     6601 gcgatcaaca ccatggtcgc cacgcgattg tcggaatacc tgcagatcca cgcctcggtg
     6661 gtggcgcgtg acggcgtggc gatcatttgt ccgggtcagc cgggacaagg gaagacgacg
     6721 ctggcggccg ccctgctcag ccggggctgg tcttacttgt ccgatgagtt tgccctgctt
     6781 gatcctgaga cgcggctgct ggaaccctat cccaaggcat tatgcatcaa ggcgggatct
     6841 ttcgaggttc ttcggcgctt gcgcctgccg attgataccg accgcgttta ctacaagggc
     6901 gagaaggggc aggtaacgct gttgaatcct ctgaggatcc gccccgacgt ggtcgccgac
     6961 cgttgccggg ttggaatggt ggttttcccc gagatcaatc gctcgaccac gccggtcatc
     7021 gaacgcgtgt ctcgggccca gacggttttt gaactgactc gctgcgcctt caacttcggc
     7081 aggttccggg cgacgggctt caacctgctg gcggatgtgg ttgagcgggc cgggtgctac
     7141 cggcttcgct gcggtcatct cgcaacgacg tgcgacctgg ttgaggcttt ctttactcgt
     7201 gaagtcgggt cgggggtgta gcttggcgca cggcattgca gacccttcga ctgatcggct
     7261 taatcggctt gtggcggatt gcctcacgga ccgaaccctt atggcggtcg aggggctctg
     7321 gcgcgacggt ctgtggagcc aagtcgtaga tcacgcatgc tcgtcgggcg tggggggcgc
     7381 cgttgcccgg aacatggcag cgcgagaggt ggaaatcccc cggtcggcgg caatccagct
     7441 tgattcctac aggcagcacg tagcggcggc agcagcctac ggcctggaga agaccgaacc
     7501 gatactcgca tgcctgcaga aagccgggat tgcctttctg gtgctcaagg gggcggcgct
     7561 gaacgccgcg ctctacgacg gactcggcct gcgaccgatg atggatgtcg atctgttgat
     7621 ccgcccatct gacgcgcagc gagccgacgc cgcactcaca agccttggct gccgtccggg
     7681 gcccgatctg gtcaacgaag acttctaccc acgtttttac tatgagcggg agtacttcct
     7741 gccctgccgt ccggaagtca agattgattt gcatgttcgc ccgttccggc cgctgcgcta
     7801 tgcagtgacg ctgccggatc atgccatgtg ggatgagccg atgccggtga ggtacgggcg
     7861 attgacggtg cagattccca acgtcgagaa catgctgatc cacctggcgg tgcatgcggc
     7921 ctgtcacggc gcggcagagt taaaatggct ttatgacctc aagctgtggc tggatcggta
     7981 cggcgaccgc ctggacatca ggaggctggt ggacaagtgt cagagatgga agatcggttg
     8041 gccggtacgc gtcgtcctcg agcacgttga cagcctgttc ggcgaaggct cggtgtatct
     8101 ggaacggacg ctctccgcgt tgaagggttg ccgcgccggg tggagggaca ggcttgcctt
     8161 ggttcagagt ccgcgcgacg ccgcacaccc gctggccgcc gtcgcagtga acgtgctctg
     8221 tacgccgggc gtccgattca agcttggcta ccttcgggcc gtgctgctcc ctgcccgatc
     8281 gcacctggcc caactctacc acggccgcca tcccggctgg ccgctggtgg cccacgccgt
     8341 tcggatcggc cgtcgcctga cacggcgatt ctgccgcact cgccccgcat ccgtataaga
     8401 cgatacgggc tgatctcgct tccccgacac atctgtcgta tcctatcgac tcttgtgaga
     8461 ttacggttca attgcagtca gacgaggtgg agcatggagt ccaggatcac gtttgtggcc
     8521 gcagcagcgg tattcatggc gtcgatggcg gttgccggcg agatggggat tgcgaagatg
     8581 aaggcggttg cgccgacaca ggtcaagctg actgattcgt tctgggcgcc gcggctggag
     8641 atcaaccgga cggtcacgct gccgcacaat atcaagatgt gtgaggaaac cggccggatc
     8701 tccaacttcg acaaggccgc cggcaagatg caaggcaaat acgaaggtgc ctatttcaac
     8761 gactcagacg tctacaaggt gcttgagggg gcagcctaca tactggccgc tcatccggac
     8821 aaggagatgg acgcccgcgt tgtcgacatc atcgccagga tcgcggcggc ccagcagccc
     8881 gacggctatc tcaacgccta ctacacggtc atggagccgg acaagcggtg gaccgatctg
     8941 ccgaaaatgc acgagctcta ctgtgccgga cacctgaccg aggcggccgt ggcgtgggct
     9001 gacgcgaccg gcaggaccga cttcctggac gtcgcccgca gattcataca ccacatcgag
     9061 ggcatttttg ggtttgacaa gcggcacggc gtgcccggcc atgaggagat cgagctggcc
     9121 cttgtcaagc tgtaccgcaa gaccggcgaa gagcggtacc tcaagctggc ggagtatttt
     9181 cttgccgccc gcggcaccaa acccgaaaag ggcgctgagt accgccaaga ccacatcccg
     9241 atcaagcaac agaaggagat cgtcggccac gcggtacggg cgatgtatct ctactcgggt
     9301 gtgaccgacg ttgcggcgat taccggcgac aaggaatacc tgcagactct cgacacgata
     9361 tggcacgacg tcgtgactcg gaaaatgtac attaccggcg gcgtcggagc ccggcacgaa
     9421 ggcgaggcct tcgggggagc ctacgaactg cccaacgact cggcatacgc cgagacctgc
     9481 gccggcatcg gccttgcatt ctgggcccac cggatgctga tgctgcacgg agagagccgt
     9541 tttgccgacg tgctcgagcg agagctgtat aacgcatttc cgtcctcggt ctcgctgaaa
     9601 ggtgacgcgt ttttctacgt caacccgctt tcgagcaagg gcgagcacca ccgccagcca
     9661 tggtacggct gcgcctgctg cccgacgaat atcgtccggg tgatgccaca ggtgccgggg
     9721 tatgcttaca gccagtctga cgacggcgtc tgggtcaact tatacatggc gggtgagggg
     9781 accgtcgcgc tcaagtctgg tgaggtcaag ctggctgttg acacgaagta tccgtgggag
     9841 ggcaaagtga agatcaaggt gacgcccagg gagccggccg agttcgcgat caatctccgc
     9901 gtacccgact ggtgcgagca ggttacggtc agtctgaacg gaacggcgat ccccaacccg
     9961 accatggaca agggatatgc ggtgatccgg cggcagtgga aagccggcga cgcgatcgag
    10021 atggacatgg cgatgccggt ccggcgaatc caggctcatc cgaacgtggc cgcaaaccgc
    10081 gggcgggtag ccctccaacg cggtccgatg gtcttctgtc tggaagcggt cgacaacggc
    10141 ggcaaggtgt ttgatctggt tctgccgcga gatgccgaac tcaagacaga gttcaggccc
    10201 aacatgctcg gcggcatcat gacgatcaag gcccagggcc tcaggtgccc ggctcgcgac
    10261 tggaaggaca agctgtatct ggcggcacca gcgcatgccg aaccggtcga gctgaccgcc
    10321 gtgccctatt gcatctggga caaccgcgaa gcgggcgaaa tgaccgtatg gattccggag
    10381 atcaccgggc tgtccaactg accagccagg ggacaaggtc gcggttgtga ccggagtggc
    10441 agcgcacacc ctccccgccc tgccggtgcc ttgaccgtta cgcgcagatg tcggcgggcc
    10501 tggcgttgag gaggcgcacg acgtcttcgg tggccatctc cacgagcacg ccgcgacggc
    10561 cgccattaac gatgattctg tcgagggcca acaatgattc ctcgatgacc acgggcaact
    10621 ggcgccgcat agcgaacggc gagattccac cgacgcggta accggtgatt ttctcggcct
    10681 cgtcatgctc ggccagttcc gcctcggccg caccgatggc tccggccatc tttcgagtgt
    10741 cgaatcgctg atcgccgggc acgatggcca cccagaaggc cttgcccaca gccttgacga
    10801 tcagtgtctt gcagcacgat tcgagcggac ggccgacggc ctcggccgca cggtcggctc
    10861 ccacctcggt gaaggtgtat tcgagcacct cgaatggtac gccctgggat cggagccact
    10921 tgaccgcatt agtatcggac atggtcttct acgttgcgcg agcctttcaa gaacctgccg
    10981 tcggtatccg caaaatactt caggaggcaa aggcaatgcg gtcgatcgcc aaaccgttcc
    11041 tgcggcaatg ctcaaccagg agcgagacat agctctggcg gacggtccgg cactcgccga
    11101 agcccagagc ccgctgggcc tgcacgacgg cagcctcatc aggtccctcg atctcgatat
    11161 agcggcccag gtagggaatc tcatcgagtt cgatcgtgca gtcgcccagt cgccaggttt
    11221 cccgccgttt ttcgaagcac aacacctcga cgaaccccag ggcctggatg agttccttac
    11281 aggcctcgcc gtcgtccgtt gcgatctcga tttcctcgcg ggtcttgagg ggcccctgga
    11341 tacgaggtcc cttgtaggtc agcgtagcag gcgggtcatt gccgtcctcg gcccggcaga
    11401 ttcgaacccg caggccgcgt ccgctcgaca acagcgtacg atcccggttg tcgaagatat
    11461 gattggtctc caacacccgg cagattcgca tggctcgcaa cgatgagagc ctttccaaca
    11521 cggcttcgtg cgaatccact ttgatcttgg cttccacctc gacaggcatg ggtttctcaa
    11581 cgcatgtgcc ggcatcaacg tcaagggcca cccccaccgg ccaatcgacc acctcgctac
    11641 gtcagggcag ccctcacttg gtcgcggtct tgagacactc caacggattt gggagcggct
    11701 ccgtccagaa ccagtttacc tcgatcgaaa tgcccggcaa gatgcgaccg tcgaaccgtc
    11761 cgttggtgaa cgtgttggtc aggtggactt cgccttcgag cacgttcacc tgaacctgct
    11821 tgtggtccgc gttgatgaac cagatcttcg ggatccggcc ctgatgacag gcggcgtgct
    11881 tctcgcccaa gtcgtactcc cgcgtatctg taaatgccat ctcgacgacc aggtccgtcg
    11941 gcccgacgac ccgcgtcccg cggatgcgat ccgtgtgctc ggccgccaga taggacaagt
    12001 ccgggcaaaa ccgccgctcg ccaagctgca tgaccgcatt ggaggcgtac atcctaccga
    12061 gccggcgtcc ggcaacgacc gtgttcagca gcgtggccag gaatatgagc ctttcttcgt
    12121 gttgaaccga tgccggtgaa tgcacaatct gtacttcatc aaggtactca catcgcaggt
    12181 cttcgtgggc cggctcttcg tagtcctcga ttgtcgcctc gtatttgcgc agctgatagg
    12241 gcggagcaag atgctggagg tcttctcgca gctcacggat gtgcttggca tcaagtgtca
    12301 gcatgagcca tctccggccc attatagcga ttcggtgctc cacaagacag tcacgagttc
    12361 gcggccgagg ccgacggagc ccatgacaga agcggcggca cttctcgcgg ctgacccggt
    12421 tgtcgtgaac gcaagccaga ccgcttcagc ggtctctccc gaagggcaaa caggccggcc
    12481 gtttgacgac tggttcacgg tcggccaatt cctccttctc tgtgttaccc catcaagccc
    12541 gtcttaacgg gcatgtcctt gtcaggtaag cccgttgaaa cgggctgaga ccatagaaag
    12601 gaagaagaac cgcgggcaac cgcaaaccgg ccgtatcacg gccggcctat cagcccgtct
    12661 gtggcggcaa acccccctga agggattttg atgccagcgg aaagagactg ccgacgaggg
    12721 cgttggcacc actggatcag gtcgacaccc acaccactgg gtcagttcga catcggcacc
    12781 agtggatcag ctcggcggca ggaccgcctc gggcacggcg ctgggcacct tgctgtcggc
    12841 ggccgagcgg atgcgccggc gggcggaggt tgcgggagca tcagactcgg gctggctggc
    12901 gcgaggcacc gcgatcaggc ctgtccgcga cagggctttg atcccatacg gtcggcacaa
    12961 ctcgacaaat gcctcgatct tgtcctccgg gccggtcaac tggatcacga tcgaccgcgg
    13021 gccgacatcc acgatgctgc cgcgaaagac ctcggtaatc tgtctcaact cgacgcgttt
    13081 ctcgggcgga cagtgcacct gcaacaagag caggtctcgc tccacgcagg actgttcgct
    13141 caggtcgcgg acctttacca cctcgacgat cttgccaagc tgcttgcgga cctgctccat
    13201 ggtctgatca tcaccggtgg aaacgatgac catccgcgag aactcgggat cctcggtgcg
    13261 gcccacgacc agcgagtcga tattgtaacc cctggccgcg aacatgcccg caatgtgggc
    13321 caacacaccg ggtttgttat gcaccaacac gctcagaata tgttttctca tgattgcctc
    13381 gtagcctctc gccggggaat tggcccggcg ttcaatgtcc catttcatgc gcctcgttgc
    13441 acctgggacg ccggctgaag gggaactccc cccatctccg cggccctggc ttggggaacg
    13501 atcatcttga ccggatgaac acggtcgaaa cggacgttct cgggcagcac caggccgccg
    13561 gacatcaccg ccttgaccgc ctcctccacc gtccagtcgg tctccgacac ttcctccagc
    13621 ggcacaagct gcgtgtagcc ggtggaacac gtcggtgtgc tggcgatgaa gattgcggcg
    13681 atcttgcggc cggtgtccgc atcgtccacc accgacgtga gaaagccgat gcacttcatg
    13741 cccggccgcg ggtactcgac caggacgacc cgctggaagt aactcgactg gcccccaccg
    13801 accgtcagca cgatctgctt ggtcgagcgg tagatggttt tgaccaaggg cactttgccg
    13861 aaaatgccgt ccaaccacag caggaaccgt gccccaaaca cgttcgccga gaacaaaccc
    13921 aggagataca gcaggaacag cgtcagcaaa acggtcaccg ccgggacgac ccattcctgg
    13981 tagtcgttga ccgtctgcgg cagcagcgcc ttattgtgtt ccagcagcgc cttggccacc
    14041 cggccggcca gcggatccat cgcggagcgc aggttgtcga aaacgaattt cacaacaacc
    14101 cacgttaccc agatggggat caacgtcagg agaccggcca cgatgcgcgt ccgcaggagc
    14161 gtacggatca tcggccacgg tcgacgcgga tcctgcgacc ggggaagttc gttccgctga
    14221 tcggttctgt cttctttgcc ggacaccgcc gctctccggt gctgtgtcag gacatgccgc
    14281 ccatttccat ctcgtgcagg ctcttgccga cggccaccat cggatagaca ttctcctcgc
    14341 gggcgacgtg cgcgtccacc acgacggggc cgggcgtgtc aagcattttc ttgatcgcct
    14401 tgggcaggtc ttgcggatcc ttgacctcga tgccggtgca tccgaaagcg tcggccacgc
    14461 gcgcgaagtt ggggttgacc atttgtgtgc cgacgtaccg gcgtccgaag aacagctcct
    14521 gccactgacg aaccatgccc tggaacgcgt tgttcaggat aaccaccttc acggggatct
    14581 tgcgctcggc aacggtggcc agttcgctgc aggtcatcag gaagctgccg tcgccatcga
    14641 tgtcgataac aatcttgtca gaaagaccga tggccgcgcc aatggccgcg gggaaaccat
    14701 agcccatggt tcccaggccc cccgaactga tcatctgccg cgggaatcgc cagcggtaga
    14761 actgggccgc ccacatctgg tgctggccca cgccggtggc aatgaccgcc tcgctgttgg
    14821 tctgacggca gatttcctcg acaaccgcct gcggcttgat gacgcctgct tggggctgat
    14881 agctgaaggg gaagcgtcgc ttccactcat tgatctgggc gaaccactcg gcacgttcgc
    14941 gccgctcgat caaaggcagc atttcgcaaa gcacttccct ggcatcgccg acgacgggga
    15001 tatccacatc gacactcttg ctgatgctcg atgggtcaat gtcgatatgg atgatcctgg
    15061 catggggcgc gaaggtatcc agcttgccgg tcacgcggtc atcgaatctt gcacccacgg
    15121 cgatcagcag gtcggacgcc tggacggcgt agttggcgta cgcgcttccg tgcatgccca
    15181 acatgtgcag agccagatcg ctatcggcca cctcgtcgaa cgcgcccagc cccaacaagg
    15241 tagtcgtcac ggggatgttg ccggcacggg cgagcttgcg cagttcggcc gaggcatttg
    15301 acgagatgac gccgccgccg acgtaaagga ccggcctctc ggacgcgttg atggcttggg
    15361 cggccagccg gatctgccgc tggttgccct tgcagcgtgg ccgatagccg ggaagaagcg
    15421 gctccctgtt gttgccatca tcggggatgg agacctgcac atctttgggc aggtcgatga
    15481 gcaccggtcc aggacgcccg gttcgggcga tgaagtaggc ctcgcggatg atccgctcga
    15541 ggttgttaac gtccttcacc agaacgttgt gcttggtaac cggacgggtc acgcccacga
    15601 cgtcggcctc ctggaaggcg tcgtttccga tcgcggcggt agcaacctgg ccggtgatgg
    15661 ccaccagcgg aatgctgtcc atgtaggccg tcgccagagc cgtcgtcagg ttggtagccc
    15721 cgggaccgct ggtgacaatg accgttccga ccttgcccgt ggctcgggcg taaccatcgg
    15781 ccatatgccc ggcgccctgc tcgtgccgac tgacgataaa ccggatgggc gtatcatgca
    15841 gggtgtcgaa cgtgggcaag atggcccctc cggagtaacc gaaggcgact tcaacaccca
    15901 agtcaaccaa cgtgtcgtgg acaatcctgg cacccgtttt tcgggccggg ggtgaagatg
    15961 ctggtcggga cgacgtaact gtaggcgcag aaatggtttg cggttgcgcg gccatggttt
    16021 tctcctttcc aataggtttc catggttgcc accggggcac cgcaagcacc gatggccgag
    16081 cctgaccggc ttcaaccggc cctgctacgg ggttgctccc acggcaaccc gcgacaggcg
    16141 gctcgtaatg tcgaattatc gttcaaatac cggggtccct tgaaccccaa acggtctgcg
    16201 acgcaggccg tctggtggta ttcttacaca caaccgtcgg tattgcaagg gctgcagggc
    16261 cgtcgcgcag ccctgagcct cacgtccttt gccgaatctt aggtccccac cttggcccag
    16321 accgtattgc cgctcgcttg ccaagccgac gttttgccgg tctactcagc ctgcaccgtc
    16381 ggcaatcgaa gactggactg gaggtggcac tatgtggcga ttcgcacccg cagttgtgat
    16441 ttgcgcgatc gtggcgggga gcagccaagg cgcgcctcgc aacgtggtgc tcatgatcgg
    16501 tgacgaccac ggcatgcagg tcggttgtta tggcgacaag gtcatcaaga ccccctccat
    16561 ggatcggctg gcatcacagg gcacgcggtt cgccaatgcc ttcgcggcgg tctccagttg
    16621 cagcccgtcg cgagcggtca tcctgagcgg gatgttcacg cacagtaacg gccagtacgg
    16681 cctggcgcat gccacgcaca atttccattc gtttcacgac gttcaatcgt taccgaggca
    16741 gctcaaggag gccggttatc gaaccgcgat catcggcaag taccacgtgc agccggagga
    16801 aacttatccg ttcaccgaga cgctgccctg tccgggcggg ccgcgaaacg tcgccgccat
    16861 ggccgagcag gcccgcaggt ttatgggggc ccaaggcgac agccctttct tcttgctcgt
    16921 cggctacacc gatccgcacc gcgatgcgaa aggctttgcc aacaggccca aatacgacgg
    16981 catcgagacg atcacctata agcccgaaga catgattgtt ccgccctggc tgagcgacca
    17041 cccggaggtt cgcaaagagc tggcagaata ctaccaatcg atcagccgtc tcgatcaggg
    17101 cgtcgggctg atgctcaagg cgattgccga caccggtcac gaaaacgaca cgctgacgat
    17161 ctatgtgagc gacaacggtc cgccctggcc gggggccaag accaccttgt acgaccccgg
    17221 tattcactta cctctgatca tccgaagccc gcagcagaaa aaagccgggg tcgttaacga
    17281 agcgatggtc agcttcgtgg atctcacgcc gaccatcttg gaatgggccg gcgttgagaa
    17341 gccttccaag gtgttcggcc gctcgctgtt gccgatttta gaggaaacac gaccggaagg
    17401 ctgggacacc gtttacgggt cgcacatctt ccacgaagtc accatgtact atcccgcgcg
    17461 gatgatccgt acccgccagt acaagtacat cctcaacctg gcacatcgtc tggagttccc
    17521 tttcgcctcg gacctgtggg ggagcgacac gtggcagggg gtgctgaaac ggggcgacaa
    17581 ccagtatggt aagcgaacgg tggacgctta catccatcgc cctcgcgaag aactgtacga
    17641 tctccaagcg gatccccacg agacgaaaaa cctggccggc gatccggcac atgcccagac
    17701 agtggccgac ctgcgcgcac ggttgctgga atggcagaag aagaccgaag acccgtggct
    17761 ggtcaaatac caacacgaat aaggctcttg ggaggtccac ggggggcagc tcttcagtga
    17821 ctcgaagaag gcaagaggct gacgggaaga gttgtcggtc gccagttgtc agttcccggt
    17881 ctcgcgcacg gcaaccactt cagaaccccg aacgggcgtt gcaggtaaca gcggcgtcac
    17941 ggggtcgaga aacatccagc agaacgttcc catgaaatag gcaccggcaa acgtgtagag
    18001 tacgacgtcc caattatggc ccgtccgcac cagcagaaaa cccgtggcga gcgggccaag
    18061 gctgccggcg agattgccca tcatgttcat gccgccggag agcgtgccgc aagccctgcc
    18121 gccgacgtcc atacagaccg cccagctcgg cggcaccgca aggtccataa agaagctggc
    18181 caggcccatg acgatcattg ccgtaagggg gctgtggata ctcacggaca cggccgtgca
    18241 cccggcggca ccgaggcagc ctgcgcaggc catgaggcgg cgaatccttc gggcgtttcc
    18301 gccttttctc tcgaagtggc cgccgatgaa cccgctgcac agggagccca cgccgccaaa
    18361 caacagcggt atccccgcca agacggcacc agtcccgagt tgaaggccac gagcttcctt
    18421 gaggtaggtc ggcagccagg tgatgtagaa ggtccagccg tagctcagga aagcgtattg
    18481 agcacagagc agccagacgg tttttgatct gagcagtttt cgccagggca cggcctgatg
    18541 aaccggcgcg gtctcagagg cagttgggat cagggccagc tcggcggcat tgacgtgggg
    18601 gttatctcgc gggtcgtcgc gataccagcg gtagaagaag acggcccaga tgacacccag
    18661 cagtccgaaa gcgccgaaag cctgtcgcca ggaaaggaac ttgagtacca gtatcaccag
    18721 caaaggggta aaggctccgc cccagcgggc gctgagccac atgagtcctt gagcgcggac
    18781 ccgctcgcct cgcggcagcc aggtggcgaa agctttggtc acattcggga agcagccggc
    18841 ctctccggcg ccgaacaggg ctcgggccgt gaccagggag gccagattcc atgcccagcc
    18901 ggtggccacg gtgaagaagg accaccagac tacgacccgc atcaggactc tgcgggcgcc
    18961 ccaccgatcg cccatccagc cgccgggtat ctcaaacaag gcgtatgtcc agaagaacac
    19021 cgcaaaagcc aggcccattt gaacttcgct gaggccaaga tcctgccgaa tgaatggtgc
    19081 cgcctgggaa atgcagaccc gatcgatgta ggtcacgacc gctagcgtca cggcaaagac
    19141 aatgacccag aagcgggtac gcgtcggggc gatgggcggt gccttcgaga gcggttcctg
    19201 agcggtctga atcatgccga tgcaatctaa ggcggaccgc ctttttgggg gccgcaaccc
    19261 aaccgtccta cgatgcagaa acatgcgtgc aaaccgcaca gatgtccagc cgtaaggtgc
    19321 taaccatccg ggaccgggac ggcaaagcgc ccgaagcaac caagatcaca gcagcgatcc
    19381 tgccgagccg cttctggtca cgcagagaac gctcagccaa taacgttctc gctgatctcc
    19441 agagcggtgg gcacgtcagg gtggtcgaag gtgtagataa tgatatcccc gcaaccaaag
    19501 tcgccaccgt ctcggagaat atccgtgctg gcactgggac caacggttcc gacaaggctc
    19561 aggtcggctt tcttgatcag aatggcctga aggtcctcac aatccttgaa gaacgtgacc
    19621 gcctgaccgg gtgggatagt gtaggtcagc tctgttccaa actccctgag caactcctga
    19681 agcacatact gattgttgct gattcgaagc tcgacgatga ccgggtaatc gccgttgttg
    19741 accagttgga cggtcgtggc tttgccctga agcagtttca tcaggtcgca agccccgacc
    19801 agcggcaata ccaacccgac caaggcaaga caggcaagac gtttcatcgc ggtctccttt
    19861 ccgcagcgca aatggccttc catgagatct gccggcaccc cgcagcctgg ccggcttgcg
    19921 acaattatac ctccgaaacc gacatcatga cgagggagtc aggtctgctt ggattgccac
    19981 acggcggctt tcggcacgag tgtcacacgg ggttcggccg ctggatccga tcggatggtc
    20041 gattcggcat atgccgaatt gccgacggtg gaccggccag accagaacgt ccgcatctta
    20101 ccgattccga atccgaaccg tccagcgcaa gtactttagt accatgagga tgcggttttc
    20161 caggaaccaa ctgttccttt ggggcatctc cttccagaac gggtgagaac gttgtacaat
    20221 agggcatcaa ccccgttcca aggaccgtga agggacaggg gtggttgcgc gtccgatcag
    20281 ccgcgcgtcg ggtaaaggat gacgctcccg cgcgagaggc gatcacttgg ttcggtcggc
    20341 accgagtcct gcgaggactc ggcaacaacg gcccgcaaca cgtcgcagtc gcaggtcaaa
    20401 cgcgaggaag ggaacgaacc catgcgcccc aagtcagttc tgacaaccgt cattctttca
    20461 tcatcaatgg tcacggtctt tgccaaggct gatctcacct acaactatga gatcctttcc
    20521 gccggagtcg aggcgggggg gtttgttctt cctgacgaga ccttgacgtt cgtggagcag
    20581 gtttacgacg acacgccgga gccctacaac caccaggccg agctgatcac caatacactg
    20641 accggcagag ggctcagcat cctggatgtg tcgattgctc ctgacagcta cgactttcag
    20701 cacattgacg cggcgctgct tgaccttcag gccgagctca cggagacggg ctattcgaag
    20761 gtttttgctc aaggcgacag ctatttccag ttctgggtcc aaccaaggga caggaactac
    20821 gagtatgttg tcagggtgga gcaggaatat gatgatgggc tggtgccggc gtatcttgcc
    20881 ggaggcgacc gtgcctttga cctgtttgcg gtcctggaag gtgtcggaaa cacgaccccg
    20941 ggcgaagcgg gcttctggta tttcgtcgga acacttgccc cgggggttga ttaccactgg
    21001 tacggcggcg tccgggcagc agccgagagc aacggccttc tatcaggtcc cttcaacgcc
    21061 cgcttacgga tggatttcac cctggtgcct gagacggatg actatctttg gatcggaccg
    21121 gaccgcggac tgttcgccga aagctcgttc tgggacccgt caggcacccc ctgggaaaac
    21181 ggtcgcgcga tcttcaattc gccgagcggc gccgatcaga ccgtcctgtt cgacgacgat
    21241 caccggacgg gccgactggt ggtgaccgcg ccggcgggcc agaacactga cgatcagatt
    21301 gtgttggatc tggatggctg gaactactgg atactcaggg aatggaacga cgtataccgg
    21361 gcttctctca ccgtcggcaa caccgcaaat gcgacggcca acctggccat tgtcgacggc
    21421 cacgttgacg cgaaggacgt cgagatcggt cttaacggaa ccgggcagct gaccatcggt
    21481 gaggacggcg tgttcaccgt cgttgctcac ggcgtcagcg ttgggactca agactatggc
    21541 tcactgcaca tcgttgacgg tggctggatg catcacggtc acggattcgt cggccagttc
    21601 gccggctcgc acggcacgat caccgtcgac ggcacgaagc cgtctgaccc caacgtgcgt
    21661 tcctattggc aggccggcgg ctggctcggc ctcggcgatc agggggaggg tagcttgtat
    21721 gtggccaacg gcgcggacgc tgaaacgggc aagtgcgaca tggcctgcaa cccgggctcg
    21781 acgggcatgg ccgacatttc cggcgacgga tcacaatggg agttgcagtc ccattgggag
    21841 gtcagtctga tcgtcggcaa gtccggatca gcttctgtga ccacgtggga cggcgggtcg
    21901 gtggtcaatc gaggcgaaat gcatctcgcg gaaacgccgg gcagcacggg gacgatcgtg
    21961 ctcgttggac cgggcagaga caattccggc gcgacgatcc cggcactgga atgcgaacag
    22021 cgtctcaccg tgggctacgg tggggttggc gatttcgagc tatgggcgga tgcgtgggct
    22081 gtcgtccacg ggccggtcat tgtgggcacc gacccgaatg ccgaagagag cagctacagc
    22141 caggttatcg tggacggggc cgactctctg ctggaagtct ccggcaagga tggtgcgggc
    22201 agctacatcg ggatggagat cggccggcag ggcgccggca atctgctgag catcaccaac
    22261 ggcggccaag tcaccaccgg ctatacgacc acaatcggcg gtggcggcgg cgacgcgacc
    22321 ctgctggttg acggtttgga ctctcttctg ctgtcccact ttcagttcga ggttggccgt
    22381 gacggcccgg gcgaggtaac ggtgagcaac ggcggcagga tctatgtcga tggctccaac
    22441 acggacccgg agtgggacga cggtttcaag ggcctcactc acatcggcat gcgttcgacc
    22501 gggatcctga cggtcacggg catggatggc gggacgcctt ccacgttgga gagcatggcc
    22561 cagatctcac tcggctcgaa cacgggcggc aatggaacgc tcaacatcga ggacggcggt
    22621 ctcgtcgtct cctggaagca cgtttccccc actgaaagtg ccggtatcgt cggccgaggc
    22681 gcagggggca ccggcctggt aacgatcaag gacggctcta catggcagat gcctgatggc
    22741 ggcttgaccg tcggctggat gggcaaaggc cgtatcgaga tggcgggcgg ctacatgttc
    22801 tcgacctatg gggtcatcgg ccgaacacct ggctcgcagg gtgaggtgga actgtccaac
    22861 ccgaccgggt cagatccctc tcattgggat atcgatggcc cgctggccat cggcggcgat
    22921 ctggccggga cggtcggcgg caccggccgc ctgttcatcg ggcgggactg ctgggtatgg
    22981 acgtcgggcg gcacaaccat ctacacctcc ggaacactat ctctagccga gggcggaatc
    23041 gacactgata tcacacttca cggaacgatc gaaggcgtcg gctggattgc tcgccccgtg
    23101 accaacgttt ccggcgtggt gcacccgacc tatacgggga cgggtgctct tcgcgagctg
    23161 accttcaggc aaggctacgt ccagcagtcg ggaggcacgt tcctggtcga cgtggccgga
    23221 gacggcgact ccagcgtcat cgtcacttgg gattccgcct actcaatctc gctggccggg
    23281 aagctgaagg tcaagatcct caacggctac ataccggcga tcaattcgag gttcctcatt
    23341 gcgcacgcgg aaggcggcat catcaacgag gcattcgaca cgctggacct gcccaccatc
    23401 gacggccagc ccgtgttcga gctgtacgcg gagggcacca gtctgctctg gctgcggacc
    23461 cttcgcgatt accatgcccc ggtcgattac gacggcgacg gcgacgttga ccaggacgat
    23521 ctggacatct tcgaggcctg tgccaccgga ccgggcatac cgttcgccga cgggaattgc
    23581 gcgaaagccg atctcgaccg ggataccgac gtagatcaga ctgacttcag caccttccag
    23641 cgctgctaca gcggcgagaa cgtgccggtc aacgccgatt gcgcgaactg acgctggtta
    23701 gactgatgac gcgggtcttc gttgcgcccg gcaagtgcga ccttgcccga ccggtgtggt
    23761 gaggtcaccg ctcgtccggg cgaatgtact cttgtccgac ggggtcggcg ccggcctcga
    23821 cgtactgctc gaaccagtcc aacatgcgtt tgcgaagcac cgttctcagt tcccgatgtt
    23881 caggcagatc caccagattg gtcgtctcct gagggtctct ggacaggtcg tacaactcga
    23941 aaggcccgcc gttggcccgg tgaatgtact tgtgggcggc ggtgcggatg tagcggagct
    24001 ggccgtactc gcctagtacg gcgtcgggcc agtccggctg cttctcgccg cgaagcatgg
    24061 gggcgaagct gccgccgggc agattccggt cggtcggggt ctttacatcc aggtaatcaa
    24121 gaaccgtggg catgaaatcg tacgcactga ccatcgcgtc cacggtcgcg ccggccggaa
    24181 gacgcccggg atggctgaag atcatcggca cgcgcagtga ggtgtcatag gcgtttcgcg
    24241 gattggtgcc gttgcccttg ccccaaaaac cgtgatgacc ggtacaaaag ccctggtcac
    24301 tggtatagac caccagcgtg tcacgaatca gccccaacat ctcgagttcc gccagcaggc
    24361 ggccgacggc ccgatcgacc cccgagcagg cggcaaaata ctgctcgagc ggctcgcgct
    24421 ggttgtggag gctcgtcaaa cccgaagcag cccacgggtg cggcgccagc gtggggattg
    24481 acgcgaacgg gacatcggca aacaggtcga ccagttcctt gggctggccg gtgtacgggc
    24541 tgtgcggggc gttgtattgg atgttcagga agaacggccg gtcgcggttg gttcttaaga
    24601 actcgacggc cttgtcggtg atgcgatccg tgatatagcc ctcggctttg accagctcac
    24661 cctgccagat catctcggcg tcgttgtact ggctgccgcc ttgtggcatc gcgtaccaga
    24721 aggtatagcc ggcgcgggcg tggaggctgt cgccgagatg ccacttgccc gacaggccgc
    24781 agacatagcc ggcctcggcg agaacctcgc tgatctgttt ctcgcggggc aggcagtacc
    24841 gtgctcgcgg ccccatgttt tcatgcttga tccagtcatg gatgccgtgc tgggaaggga
    24901 tccgtccggt gaagaacgtc gcacggctgg gcgaacagac cggcgtcgtg gcaaaggcac
    24961 gcgtcatgcg gaccccgccg gcggccatct tgtccaggac gggcgtacgg gcctctgaat
    25021 tgccgtagca acccaccgac caggccccct ggtcgtcggt catgatcaga acgagattgg
    25081 tccgggtgcc cggggcggcc gtcgcctgcg accccgcggc caaagcggaa acaaccatcg
    25141 tcacgaggcg ttgttttcga cccatcgtgt gcgctccctg ggttgtgagc ctgtcttccg
    25201 gaaagcaagc atcatcgtag cgacgtcgcg accgcagcgg aagcgatggg ttgaccgctt
    25261 tgcgccggag catacaatcg cccatgccaa tgtttcctga gaatcgggag ttgcaatgag
    25321 aattgcgttc gtgggcccgc cactgagcgg caagtcaacc ctgtttcggg cggtcaccgg
    25381 gcaagcggct ccaagccacc cgggactggg cgagcacctg gcggtggtga aggtgcccga
    25441 cccgcggctg cattggctct acaaccacta caagcccaag agaatggtcg aggcgacgat
    25501 cgactgcctc gatgttcccg gtttcagcca tgagaccgcc caacaacaag cggagttccg
    25561 aaagagcctt ccgcaccttc ggcaggccga cgctctggtg gccgtcgtgc gggctttcga
    25621 cagccccatg gttcccccct accgcgatcg ggttgacgct cgcgctgatc tcgaagagct
    25681 ggtcgcagag ctgatcttct gcgatctcga agcggtcatg aaccggatcg aaaagatcga
    25741 gaaggctcta accaagccga ccaagaccca cgagccggaa aaacgcgagc tggcgctcat
    25801 gaagcgatgt caggaagcgc tggaaaacga aaagcccatc tcctcgatca tcgaaaccga
    25861 tgaggaacgt aagacgcttt ccggctacca gttcctgacc gagctgccac tgatcgcggt
    25921 gatcaacgtc aacgaagatc aggcggccaa gccggcacca ttcgaatggc cgcaggcaaa
    25981 ggccaccatt gcgctatgtg cggagacgga agccgagatc gccgagctgc cgcctgagga
    26041 ccgcaaggcg ttcctggagg acctgggcgt gagtgaatcg gccaaggatc gtctgatcaa
    26101 gacgtgctac gaggccgtgg gactgatttc cttcctgacc gtcggcgacg acgaggtgcg
    26161 ggcttggtcg atcaagagag gcagtgacgc cgtcgaggcg gcgggcaaga ttcataccga
    26221 tatcgcccgg ggcttcatac gggccgagac ggtcgcctac aacgatctcc atgccgccgg
    26281 tgatatgaag gccgccaagg ccgcgggcaa agttcgcctc gaaggcaaga cctacatcgt
    26341 tcaggacggg gacgtcatca acttccggtt caacgtgtga ccggcacggg acccagcagt
    26401 tcaagcacct tggcggcgac gtcgggcgat cgcatgaagg tctcgccgat gagcaacgcg
    26461 cgagcccccg cctgcgtcaa tcgctggaca tctgccctgg tcttgatgcc gctttcagac
    26521 acgaagacca cgtctttgcc cacctgcttc gccagcatct ccgtagtcgc caagtccgtc
    26581 tgctggctct tcagattgcg gttgttgatc cccagcagac cgcgcttcct gtttggaaaa
    26641 ccgacgaccg actggagttg ctccaacgtc tcgggctcat ggacctcaat gaggctggcc
    26701 aaaccgagct cgtaagccaa ttcgagcagg tcggcgagac gagccggagg aagaatctcg
    26761 ccgatcagca agactgcgtc tgccccggcc acgcgggtct cgtagagctg gtactcgtcg
    26821 atgatgaagt ctttgcgcag cacgggaatc ggcacggtct ctttgacccg ggcgatgaat
    26881 tcaagagaac cgccgaagta ggtccgatcc gtcaggacgc tcagcgcgtc agccccgacg
    26941 gaatgataaa cgcgcgccag atcgacggga tcgaagtcgt ccctgatcag gccggccgag
    27001 ggcgactccc tcttgatctc ggcaataaga ttgacgtctc ttgcggggga cttggccacg
    27061 gcgcgtatga aatcgcgcgt cggccgtgta tctcgtaagg caaccttcaa ttcagacagg
    27121 ggcctccgcc ttttggccgt tgccacctcg ccccgcttgg tttccacaat ctgatccaat
    27181 atattgccca cgttcgagac tcctgtgacg acgacgttca cacatgccga gaaggctcgt
    27241 ttcctgaagc ggcattgtaa gcgatccgac gggtggatac gaagaggccc ccatgatggc
    27301 cgtgctcgcc gtgctctcag cgaacaccat gcaaaccatt gacctcagcc tgcttgtcgc
    27361 aggcggggtt gttatcgtgg ccttggcggc caagtggtgg caaagcggac gccgcgaccc
    27421 gctctgcggc tcaccgatcc gccttaaccg cctgaccccg gtctggttgt ggttctgcgt
    27481 gctcgcccaa ctgctggcct ggatgattgc aggccaggcg gcttcctgga tggacggcac
    27541 atcggctgag acgcttgccg gttccgcttc tcgacctgtc gcccccgtct cttccggcga
    27601 gagtgatgtt cttccaccag acagtcagcc gataccccgg gctttgacgt cgtcacccgc
    27661 caccgcggcc accgaatcga atccgtctcc caagccccga tcaaccgtgc tggcggccaa
    27721 cctggcacaa gtgctcatga tcgccgcgtg cttggtgctc gcaaggatga ctttccctcg
    27781 cggctggagg caattcggct ttggccgccg accgttgagc aaggacatgc cgtgggctct
    27841 ggcagcctgg ctggcggcct tgctgatctg caccatgctc tactggggca gtatcgggct
    27901 gtggagactc ctgtggccag actccgttcc ccccgagcac acggtgctca ctgtcgtgaa
    27961 ggacgctgaa gccgggctcg gcgtacggat catcgcgatc gtcggcgcgg tcattctcgc
    28021 tccggtgggc gaagaaatct tctttcgcgg gctcctacag agcggcgtca agaagctggc
    28081 ttttgttcgc cggggttcct ggcaacaccg ctggatggcc atcggcgtgg cggccggcct
    28141 ctttggtctg atgcacacgg tcacgcccca gcacatcccg gccttgatag cactcggcat
    28201 gattctgggg tacgcatatg agcgcagcgg ctcactgacg ctgccgatcc tcgtgcatat
    28261 gcttttcaat gccaagacgc tgctatgggt agcgctcggg taaaggaacc ggagcgatcc
    28321 cggccgtcag agcgccggca ccaggtgttt gcgcgttgtt tcaccggcct ttacctggta
    28381 gacgagcatt ccattgtgcc tcggcagagt cttgaactcg acctcctcga tctcgctgcc
    28441 gatgagccac acggccgcga agatcgcctg cttctccttc ttgcggacca tgatcatagg
    28501 gatgcttggc gaatccggct ggtcggtgga cgggtagccg cagaggatca cacgttcgat
    28561 ccccggatcg agcatccgca gccgcaaacg cacctcgcca tcctgccact ccattctgat
    28621 tggcccggtc ggtttgaaac tctgggggtc gcgaagccat ttccatgccg cgcccttggg
    28681 caactcgaaa ggctcgcgtg acgccagatc aggattaccg gactcggaca tcctggcgtg
    28741 gccatcgagg atgtgggcca cccagtcgat ctgtcgcggc ttggcaccgc agtcgacctg
    28801 gaacacgtca agcacgtact ccggggtcat cgctatggtc cgcatctggc ggatgccctc
    28861 gtagaggatg cccctgtcgt cggcggcggt gacgcgcttt tcttcgggca gatcgcggaa
    28921 ctctatgagg cgcaacatct cggagctgca acgctggtcg ccgccgtcga tcatgaccgt
    28981 gttctggctc agggcgccgc ggttgagctc gctctggatc ttgctgctga acgcgtgggg
    29041 cacggtcgcc cgcccttcga cgtcggagac cagcatgcgt ttctggccga acaacgtgag
    29101 gctgagcttg tcggcgttgg catgaacgcc gctgcggtcg tacgtcagga acgcgcacca
    29161 ggcgtcggtg tcccagtaga ggtcgtcctg cttcgatcgg aggaaggcgt acccgtgctc
    29221 gggcagcaga atcgagccga tgggcggtga gggcacgttc tgaggcagcg gtggggcgaa
    29281 gagcatcgtc accgaggggt tctcgctgtg gctgatgagc caagcgtact tggagtcgcc
    29341 ccacagcagc cagcaggttt cgtacaactt gttgtcgtac agccgctggt ggtggccgta
    29401 ggcatcgccg acgggcggga tcatgccgtc agctgacagg acgttaaaca tggcgtcgaa
    29461 cgactgcttg agcatgcggc cgttgccggc aaccagcccg tgcaggccgg gactgatgcc
    29521 caatcgccgc tgacaatcgg ccacgatcag catcggggcg atggccgcga actggtagtt
    29581 gaggctgctt tcgcaccaca gcccgtcgtc agtcaacccg tcttcgagca gggacacgag
    29641 cccgcgccgg ccatggagac aatagtcgac catctcgggg cggttgtaga acatcccgag
    29701 cagcccgagc atcatcttgt gccacgccaa gtgattgttg atgccgccgc cgacgtcatt
    29761 ctcgatccag taaacatcgt tcttggcgac ggccttggcc atccgagtca ggcaggcatc
    29821 gatcgccttt cgctgactcg gcggaagggc gggcagcagg gcgtcgtagg ccttgagcat
    29881 cgacaacccc cagccggcat aattcatccc cacgtcgaaa tgctcggggg cgaaggagta
    29941 aggagtaagg ttcttaagca gggcgacggc cttgtcggca tactcctgct tgccggtcaa
    30001 cagccaggcg acggcgagag tcgcggccgg gtgagcgtac gctccgggcc tgatcgccgt
    30061 gttctcgaag acctccggat aggtctcctc ccacttcttg ctcttggcct tgttccacca
    30121 gctttcatcc agcggtttga tctcgatctg caggtgcttg tccgcctcgg ccttgaggtt
    30181 gtcgaggatg gcccgagcct gctcgtcggt atcgaggcgc gccgtggccc gctcgatctc
    30241 gtcctgagtg aaaaagaggc gaggaccggc ggctttgatg gccgaggcgt ccaacgtgcc
    30301 gatgtcgaag ggcagcccct tcgatgcctg cgtcttgtcg gccccttcgg cacgttgaga
    30361 acatgcgagc aacatgaaac actgcatgac agtccatatc agtaccaggt tgcggttcac
    30421 tatcgcggtc ctccttgaat gacgattcga gtgcaaacgg tgggcgatga taccttcgga
    30481 aagataggtt tatcaaccgg ggcattgcat acggcaacgc tgcggagcga agaatagcga
    30541 atgcagaatc gaggatgggg cttgtttggt cgcctacggc gtaatgcacc ggtgccggcg
    30601 ttttagtgca agccaggaaa ccctgcgaga aggccgcctg cagataacct gatccgcgag
    30661 cacagaccct acataccccg tccgattgtt cctggtcgga tttcatgcaa ctgaggggtt
    30721 agcagtccgc cgttcaggat tggggttccc agggtttgcc gcagtgcgag cacgactgcg
    30781 gcaccgcata gtctcgggtg cgcgggtcca tgagcggaat gagcttgccg cattggctgc
    30841 attttcccga aggataggca ctcgatcgtt tgcacgaggg gcagggcttg ggccaaacct
    30901 cgtccttgac gtcaccggtg atctggatgg ttgcgtcggc tccgcaacgc gtgcagatca
    30961 cgttcacggc ctgggacttg tcgccggctt tcgagttggt atcacgtccg caagccacaa
    31021 gtagcattgc accgataaac aggtagagag ccacgaacaa cgtcgattgt gcgatccgcg
    31081 tcatgtttca cacctctgtc tggttcaggt ggtcggggtc ggctcggctc acggccatcc
    31141 gatgcgcgtg cccctgaccc attcgacgtg cgtgtcgccg aagattgcgt ttgtgcctcc
    31201 gccggtgctg gtccggtggc ttcccatgtc gatccgacga cttcccatct ccatatgttc
    31261 accgatggca cagtagatga ccgtcacgcc gcctaacccg gcagccttct gaaggcgggc
    31321 ctcgccgtcc gtccaccgcc ggtgaagcag gttcttgatg gccgggtcgc ttggcggctt
    31381 tccggtctgg cgtacctgat tccaggtcat gacggaatac tggggcaggc cgtctgtagg
    31441 ccgggaccag cctacgggtt cagagtagac ctgctgatct cgaacgatgt cctcccagag
    31501 ccacgtctgg tacgactcca tgacaccgtt ccactcccac aacgtatgcg gcggtgcgaa
    31561 tccgacgtgc cttaagtccc aaggttgccg gccccgctca aacgggcaga cgtagtgggc
    31621 tgggagaaag cgtgcctcgt agtcgtcgcg ggtgtcagat gaccaagact cgtttggatc
    31681 gaagtgttgg ccggtggtag aaacgcgctt cgacagtccg aagaggccct tttcaacgct
    31741 gcgaagggcc acagagagaa agactgcccg tgccggagcg ttgtacacgg gcccggaatg
    31801 ccagttcagc atgatcggca cgtatccctg agcctccgcc tggtagatgc tcattgcggt
    31861 tgcgatctgc ttggcgcttg ccttgcagtg cgtgagcttg gcctgattgc gtgctccctg
    31921 gagcgacggg agaagaatcg cgaccagaag tgccaggatc gcaacg
//
LOCUS       DAOTPW010000027        31956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1484_length_31956_cov_5.546472, whole genome shotgun sequence.
ACCESSION   DAOTPW010000027 DAOTPW010000000
VERSION     DAOTPW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 31956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31956
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1484_length_31956_cov_5.546472"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="P5316_04055"
     CDS             <1..1466
                     /locus_tag="P5316_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84161.1"
                     /translation="PISFSVRQSGEGGILAFTGKARGDESAIGEPFIVDYDVFYRGNL
                     VAAALDLSKAGFADKSYIKVEPVNDLAYPDWRGRPMQVELAPEGKLTVLVGSGEYGGQ
                     KEQRIRFALKITPLAAGEVQLTGVRVILRLQVSIRRQNVGFVFRSDTTNDYDRVADLP
                     PHRVVDPRGKTAPAGYRGAVGANFAFVTSLDRTTAAAMSPLTLTMKITGESVGPQFQP
                     PVLTEVAELTRDFDVSPTVSGGEIEGNTITFTQIIRPRSERVSELPALPLVFYNYQKQ
                     QYETVYSLPIPIEVRAGSLVGAQAMQVRSGTQPAWEAAVASGTAREDEPAAALGANFD
                     SLGSLVRAEPLGLNGLLLVLAGGPAIVLLVRVGIGVFQRRRPMSEVRRAQARLLTSLD
                     RLGEAERFHFELAEIVQAFLRLTFGLPPGEISADDLGRLLDERRAGSELRTAALSLLT
                     ECDAGRFAVGAVDQVEKERLIRQAREVLTKTERLAKN"
     gene            1549..2355
                     /locus_tag="P5316_04060"
     CDS             1549..2355
                     /locus_tag="P5316_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84162.1"
                     /translation="MTWISRSCFVLLVAFAAASGGARARGASAEERFAEAQALFEAAQ
                     KRLAEEKSDPVEARRMFHEAAGRFAGIAAEGVASVNLCVNTGNAYHFAGDNARALLWY
                     LRAEKLANTPEVRSGLATLRRIINAEPRPPERTSIGRVLMFWHYDLSRRTKQVLMLAT
                     YPIGCGLLLAGLMSRRRVLLRLAVTFMLMGGVVGVSDLMTAMIPPAPWAVVVEPGEGR
                     SGNGSAYSVIVPSVRMGQEVRIIERRPDWVRVEMPSGVRCWLPAETCEAV"
     gene            2457..3449
                     /locus_tag="P5316_04065"
     CDS             2457..3449
                     /locus_tag="P5316_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR84163.1"
                     /translation="MQLFKQQTRRDFLRDSLAAGAAVSVMSMNELRGAETAAKPRWII
                     ACRDAHLPETGEPDAWSAMKVMGIEGVEVSVDLAGQCSHLHGREKPYSIAKPDDVKVL
                     GDDLKKHDLKISAFCLHNHFDENLEGELACVKMTVRAARELGVPAVRLDIVPRKIKDE
                     KEFLEFAIKTGKQVVEVSKETDVRFGVENHGGTTNKPEFLKAMFEGVDSKHFGLTLDT
                     ANFYWFGHPLTKLYGYWEELAPYICHTHCKSIKYPESEREKQRPMGWEYGKYCCPVYE
                     GDIDFKRLAAILRKAAYKGDLCVENESLGRFPKEQRREILIKEAAFLRKIAEGA"
     gene            3568..3834
                     /locus_tag="P5316_04070"
     CDS             3568..3834
                     /locus_tag="P5316_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84164.1"
                     /translation="MNARRLIRGLVAALCLLLSFAFCHVTLSSGNYRSSYRDAALLAF
                     VAIVILGLSLARRPSKDRWVPEAALLAAGSYCLVYSLWRYLTSH"
     gene            complement(3989..4867)
                     /gene="rsmA"
                     /locus_tag="P5316_04075"
     CDS             complement(3989..4867)
                     /gene="rsmA"
                     /locus_tag="P5316_04075"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRR84165.1"
                     /translation="MAQTKTEIRAMLEAAGLRPLRQHGQHFLIDGNLLHKLVEAAGIT
                     RRDVVLEVGPGTGTLTDYLVEQAGHVVAVEIDRGLHEICRNRFAAAGNMTLIHGDILA
                     NKSTIDPGVLDALTRHQAKLHGRIMLVANLPYQVATPLLMDLLMGELTVSPMCFTVQA
                     EVADRLTASPGNKDYGPISVFAQATARTERITRVPPSAFWPEPQVDSAMLRINLRAGD
                     RLSPLVREQLTTIVHHCFNHRRKMLGWTLSRVLSDEHFHLVESDGRWDLSQRPEQLSV
                     EQWVQMAEMIAIRNDE"
     gene            complement(4930..7002)
                     /locus_tag="P5316_04080"
     CDS             complement(4930..7002)
                     /locus_tag="P5316_04080"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRR84166.1"
                     /translation="MMRGTRVVSIIAAVLMAFGTVSDALAAGKIKGNAGGIERSAAAY
                     SRDGRLVGWTITGPSGLYEFSGLEPGLYMLDVSGALAPCVEVVEGRTTVIDQADQPKL
                     SLEMELWGPARVRFAQSFVARGTAVTGFSLWRSSGDSKLLVSLYEDSPAGKRIAGPFE
                     TRESMTWVCGSPLPAEQFKTTPGRQYAIELAAADGKPWNHGMPRTGDVYPDGIAYYDG
                     VPHPESDLGIHIDEATPGLIQVAGADDDQHFIKEGPGSGTCTVAGQTFVATTPNILKA
                     GANCGWGGAGVQEFLFSIHEDGPGGPQVGPACTVKMVTNWGADAVWFPDAVQVTPGKT
                     YYLQYRRVDGEPFFSYLSSNVYKQGRAYRDGKLVPEQFDQLFSVIGEEEPAGIIYPVN
                     VRVSRVTDTSAVIAWETGTPGDSLVHYGTTVHLIEQAGDEQKRSRQHEVTINGLTPGT
                     AYMYRVSSHTYKKSSRRTYSRIYSFMTPPAGEDRPRFDKPPIIVEPPACDDCVAVVNP
                     GFEEGVTGWTRKAGSGRAKTPETYVPDAKPFGDATTGVEGCNPHSGKRLYGWTYRGPE
                     DPTWVEPREDWRQEVIYQRIAVEPGREYVFSAFLLTGDRGSGWGRDSRIRLAVDEDDG
                     RTLESFDTIDQANCTQWFATHHRWLPVSLHFTPKKDHVTIGVHFLNWWALETNRLYVD
                     EITVRPAK"
     gene            complement(6999..8693)
                     /locus_tag="P5316_04085"
     CDS             complement(6999..8693)
                     /locus_tag="P5316_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRR84167.1"
                     /translation="MSTQPYLLTHAAVTFSVLLLSYRASPAGTVKGRILDADTGKGIP
                     RTVVRALAHGRSVAIAAQSEGDGSYTLADLPPGRYAICLPPGETYQPVSVPDLDVPAD
                     QPAVLNLKVTRSLLIDGDSWVQPYPSFAQSFVATGLGITAVAVKGFGPGRRVTVQVVD
                     GEGPTGPHVGPPRITAPVGGESTEPVNWSGGEVPTRPGQVLTFLMTAPAGEKWVPGLA
                     GRGDVYAGGSAWFDGSPRPDSDLGIVVSEDNCGLRTDYAMSAGWHTWRLRAVGQTFTA
                     LSRSITFASAALSGVHGPPVYARFSIHEGGPGGRQIGPSKVVQVAEDAAVPWGPDEVP
                     TEPGQTLYLHIESLSGDIFLAAVQPACFASGQAFFEATPAPDWELCATVAGQISDADF
                     VRLVGRDGGSAVKLLNPSFESGIEGWLLDGPCGAVVGCDAGVTPMWGTRMFGWTNADK
                     GEGSRSFVYQAVKVVKGLSYRFSGSVYTDHDGGRSSDVKVRLTVLPGGGIQWHDNDHI
                     ETSQWYATEGQWRRASVEFTAAADTVIVGFDLEQRWNLPSSSLYVDGAFLEQVRAK"
     gene            complement(8825..9757)
                     /locus_tag="P5316_04090"
     CDS             complement(8825..9757)
                     /locus_tag="P5316_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRR84168.1"
                     /translation="MTKNPPKRLGRGLSSLMSFATPFEPPAQLENAANEPRDEKPRPP
                     AVTISVGTIRPNPFQPRKEISPNELKSLAASISSNGIIQPVIVRTKDGNVYELIAGER
                     RWRAAQMAGLSEIPAIVREASDQDMLEIALTENIFREDLNPIDRATAYKKYCDEFSLT
                     PEQVASKLGEDRSTVTNYIRLLDLPQEVKDWVTSGQLSMGHARCLLAIRSPSDLVNTA
                     RQAINLGLSVRALEKLVRDRVNAREAAAKPIRDNTAKRPQIRALEEAFTRQLGTKVEI
                     DESKRKGAGKLIIHYYSLEDFDRLAERLGVQAEI"
     gene            complement(10233..11537)
                     /locus_tag="P5316_04095"
     CDS             complement(10233..11537)
                     /locus_tag="P5316_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRR84169.1"
                     /translation="MKKTRPVGAAVSASLLLACSTLAQIPASQPVSEGLAQEQRSPAP
                     FDVLHNKRLTGDCWGLRNWLESKGIDFSLSMTAIYQHNARGGRDTHNGHDISGSVDAQ
                     LTLHFEQMGLWKGAILYVLAESSWNNDIGIDKVGNLFKVNADAYLGDYALQVSELWFQ
                     QSLLDDKVRVKVGKMDLMIDFDLNAVANDEKTQFLNPALVNMGNIPFPDYAQGIMTAI
                     QPFDWFYLRAAIADAQANGRHTGFDTFYHDECHTFSILELGLLPVWKTLRGKLPGAYR
                     LMLWYKPEPKPLLINTTDVLRHKTDDVGFAFNMDQMLWKEDPNGDDDAQGIRMFFRYG
                     YAHDDVHLIEHFWSIGAQYQGIFPNRDDDVLGLGFVQGIIGNRSSNAGLGDRESVYEI
                     YYNIALLPWLSLTPDFQWIHQPGGTHAVPDSLVVGLRMQMSL"
     gene            12017..15253
                     /locus_tag="P5316_04100"
     CDS             12017..15253
                     /locus_tag="P5316_04100"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HRR84170.1"
                     /translation="MQRLGMMLGVLTVLAAVGVCGGATVQKRYYAHETVEDRHGVIAP
                     WYQGQNGQLDLRVRIAAETLKRYPWTEPGKAPVQVPEYICSGAWKIAPDGTITIPPIS
                     DWANGDLGQRAAYVLSGLIDYYRYSGDPAAIAHIALQADYLLDYCLTPDDHPWPRFLV
                     SVPTKGKPYGQADPHGFIQLDIVAEVGAPLIRAAQMTGNQRWLDAAKHWGDLLAEKRD
                     RRPGVPPWGRYANAEDAPWEDIATGGIVFILDFFDELIRAGYTGKDNAIVEARNAGAA
                     YLRDVLLPDWTGGDVWGRNYWDWPCNVQVENVTEFAVRYLMNHPDEFPNWRNDARNIM
                     SLFLNRTSVAPGSSGDVFSGAWAYPESSGCCGRSLWYGPMELANVWAQYGVLADSEWA
                     REIARRKIILATYDVHETGVVEDNIDGGQIVAGDWFKIAHPMALKHCLAVIAWMPEIF
                     APARENHIVRSTSVVTDVSYSDGWIAYTTHDAPLNTVDVLRLSFRPTRIEADDKPVKE
                     GDTSRGIAFGVKELCDGDYLVNVRHDGATRIVIKGDDPQETIGHESFSYTGDWTRAEG
                     VDTALQRTIRIDRYSSAAGATATCSFKGNQVRLIGMAGPDGGLADIYLDDEKQLVDLD
                     CWCPQPRRGVLYHRSGLSNGDHTIRIVLRGKGNPRSGGTMVAPLEVQYSAAKADNVFG
                     EGGGPTDAQRWVFGYTGREDYIDSAGNAWRPATEFVIRSGDGVDSVKAAWWTERRQIQ
                     IDGTNDPELYRYGAHGKDFWADFTVGPGTYHACLKFCETRRIDPKLRAVTILINGEQR
                     VADLDIAATAGRDAAARTLAYPSSQPFVQPAGINHAVDLVFNDIQPRNGVISIRLRND
                     HGGEAIVQAIQVGPGDGGRGAEPVSLPPASQPAPADRTGGLLLNGGFEEGAVGDLGSL
                     GKSGGGHGWSYVFAGPTVSYIYPESAYSIHPEWGLPKFHSGKEALRTHTDGHGHTVVY
                     QDVSVVAERKYTASVWVRTDDLHGEGFGKDAGDSAGLIIQEIDKKGKVTSHPKQAVRK
                     AGDFQKVSFTFTTDKRTRTVRFILDTVIACPYDQGHVTYDDCELVRAE"
     gene            15599..16174
                     /locus_tag="P5316_04105"
     CDS             15599..16174
                     /locus_tag="P5316_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRR84171.1"
                     /translation="MPSLKGTQTEKNILTAFAGESQARNRYTYAATKAREEGYVQIAD
                     IFSETADQEREHAKRFFRFLEGGEAEVRACFPAGVIGTTAENLKQAAEGELYEWGSMY
                     PDFAKTAREEGFEAVAKVFEAIAVAEKQHEKRYRGLLANVEAGTVFKKSKKVVWRCRN
                     CGYIHEGEEAPAVCPACAHPRAHFEVLAENW"
     gene            16528..18159
                     /gene="ptsP"
                     /locus_tag="P5316_04110"
     CDS             16528..18159
                     /gene="ptsP"
                     /locus_tag="P5316_04110"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRR84172.1"
                     /translation="MGQVKANSETRFVGLPISPGLALSQVCLFNDRRHEGLKVYRIAA
                     DGVRYEQARLRKAIELADRGLRDLSARVAERIGAPEAAIFDVQRTILSDAGLSREIEA
                     MIANHRLNAEAAVARVLDSYETRLRELDDPYLRERATDIGEIRRRLLDTLGNMGSSLR
                     CWTQEHCQRGTNRIIVADELTPSLTVELDAEHIMGFVSERGGPTSHAAILARALGIPA
                     VSGIKGARELLTCGTELLLDGNTGEVIIWPDEQTLARRHCVQNSRPVPPQPVAPVQGI
                     TVMANISRADEVVDALSMQAEGIGLYRTEFEFLAAERLLDEEEQAARYASVVRAMAGR
                     PVYFRLLDIGGDKEAPFFDLPKEDNPYLGFRGSRLLLGRPDLLGPQARALARASVHGP
                     VHVIYPMIVEVEQFARLRRLFVEAIADISAGPIFHGVMFEVPAACLQAREVLEAADFG
                     SIGTNDLIQYLFAADRNNERVAYDCSPDRPVFWSLIRGIVEAAEQTGRPLSVCGELAG
                     DPRYLSRLREIGIRTVSVAPRAIPALRRSLLQPYA"
     gene            18310..18825
                     /locus_tag="P5316_04115"
     CDS             18310..18825
                     /locus_tag="P5316_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRR84173.1"
                     /translation="MASNEIRLLVLDVDGVLTDGTIFIDDDGRQSRGFHIQDGLGVSL
                     WRSVGRRVAILTSKRSPGVEARARMLGIELIEQGAEDKVPGLERLVSRAGVSLEETAY
                     AGDDLLDVAVMRRVGYPIAVANAVEEVKEIAAYVTSRSGGQGAVREAIEHLLKRDGQW
                     YAAFKAIGADR"
     gene            18857..22525
                     /locus_tag="P5316_04120"
     CDS             18857..22525
                     /locus_tag="P5316_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84174.1"
                     /translation="MFKKFMLVVASMAVIAVAYVIYQWQGEVVPVVAPRELVRLPRPV
                     PRTATQPDGAGEPAIPIGPNWITAGDAPQLRVYDKQGKARIVFRATHWSPISDSEFDL
                     LEATVRLALPGGQLVYIWADDGRITVLQGKDNNPVAKRGRLQGNVRLFIDLTDEAWRE
                     QHPDRADLDQHAESVVKLWLDRVQFDLDLSLLESDGPIVLQSALGDIEGKGLKISWNE
                     RDRRIQQLTIAEGRRAVLRSSDLAGTSSLRSKEPRPERASAARDAGLEKRSEPATRAA
                     SAPAIARIMPPAKPGGLPYIELGDRRERTAGPRVETYRLVFHDHVAAVQKEHGRRIGD
                     LTNADSLQLLLDFNQSQRGGVGGQASRPTGETPATAPASAPALAQAGEAIELTWTGPL
                     EVIPVTGDAEASSAVNRMQLIIRGPAVQFHSRDGGSGEFPELEYHDETQRVWLRGSKD
                     KPVALEPSEGRHLVAHGTVSADLRKRTARVDGPGRFTDRSEAGPGPAAVVGGRLETSD
                     FRNVEIAWEGSVDLEFAMDEQAAASAPATVNVSPIPQGAYLKHAVLSGQAVFSDDRGS
                     IRADRIDVVCGAPRKNGASSDPTSSMVVERMNAIGHVVMTVVNQDETVDTVECEHLEV
                     EFGMAEAGKSFPRQARAIGSVVARRAGRAARWGPRSVRDVVLREISAQDGLLLDMESV
                     TFSLSADEIARKEAEARARAKEQGIVEGSEEWKKHEQRLRNSLRVRSKTIARRLRAGG
                     GVTVLDLKQELDLAADSIDCELGLGREEIRKALITGSADRPARVVLGGFFIRGPRIEV
                     DAETQSAEVPGAGTLRFMTRQDLDGRAVDKPIPVVLSWDKHMSLAGDRNTGRIVGKVR
                     AVSETVILDCDDEMRLEFEDVVHPAAVSEPARTSGRWIFGMLASRVGPPARQPSDARA
                     GRQLRKRLKLLDAVGNAAIEGKVRQEGTGRETVIDQWLIAWNESLERILPADVFKPVT
                     RPADKPTRLASRVLVKGPRVIIRLDEKEMRVEGSGHLLVEDYRLRKGRRAGQRPEAGG
                     SLMDSASVASLEGLGPSQTLFTWVNSMSFLNNRNTAIFDSGVEMRHLSGSKLIELDRV
                     AAAIAVTPARLKEYPGREASLSCDRFTVEFERDAKTAAGEPAPLSRATRLKGFRALGR
                     QVRLEESGRFVEGVEVSFDGASQIGRVIGSPEFPARMRYLDERTGRPIFSQVEQFQWD
                     QRTGIIKVREPQVLSTGR"
     gene            23207..23611
                     /locus_tag="P5316_04125"
     CDS             23207..23611
                     /locus_tag="P5316_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR84175.1"
                     /translation="MENETKADERLTVREIRGDVTAQDVMEWTQLATALMRSKKYQEA
                     VELLYVAIQRDPEAGKAWFALGLCYYNLGRLSEAQEALEIARCNGGPAAQIDACLRRL
                     AMAIRAEKERSRRRAGERQTEVDLAEADRRTR"
     gene            complement(24004..25218)
                     /locus_tag="P5316_04130"
     CDS             complement(24004..25218)
                     /locus_tag="P5316_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HRR84176.1"
                     /translation="MKGSVPTELQAIADKIRAGQRLEPQEGLLLYESADIHTLGEWAN
                     LVRERLHGRRTYYNINRHINYTNYCVLRCRFCSFYRPWPERRSQDGSPATRGSLEQGN
                     CIPSDAPGYELSNDQIVEMARAAADRGATEVHIVGGLHPHWPLEHYVDMCRRIRQACP
                     QLHIKAFTAVEIVHFSRISRPRRSIREVLEALRDAGLGSLPGGGAEIFDQRVHDETFK
                     SKIGEQDWFDVHRTAHEMGIFSNATMLYGHVETPAERIAHLVKLRRHQDASLAARPAH
                     FNCVVPLSFIPAGSQLAHLPEPTGLTDLRTLAITRLMLDNFPHVKAFWVMTSTRLAQV
                     SLNWGVDDIDGTVVHYEITHRHGGQSRRQEMTADDLKRLIIEAGGIPIERDTLYREVI
                     RTTDGWTIRNPA"
     gene            complement(25228..26004)
                     /locus_tag="P5316_04135"
     CDS             complement(25228..26004)
                     /locus_tag="P5316_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRR84177.1"
                     /translation="MRLSCHAHCLRDRPLREALPSIREIGYEAVEVDWPRVQREFSAT
                     TACAEMLKAVLEENDLRLSSMSVSPVDAIDEMEIEPLLAGLTAQIAATTTLRPPAVIV
                     RAGDRRQQPFEMLTRSLRLLLDRVSGAGMALQVGNALDSTLEQIEDFRQLFMEMDHPG
                     LRLALDAGQFHCAAVNPCNVIGEFAHRLGLLRMNDRIGRRIVPLGQGEMNVRAIIEKA
                     RRSGYDGWLVIHAALPNPREGLQYLADARAYLQALLSDAP"
     gene            complement(25994..27775)
                     /locus_tag="P5316_04140"
     CDS             complement(25994..27775)
                     /locus_tag="P5316_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84178.1"
                     /translation="MNRRRQEILALALLLTGCAIVCCLGINWGLPSRDIDPYLFGRHP
                     VWSGLDIQALAGDRSVDALRPADMDADPINEPDRSVPVNQTDAQKAEIVRRFRLFTHH
                     PDEMITLMSLASMRPGRGDFDPKMYQYGGLWVYPVGALLRLAAACRVIALTPDLAYYL
                     DRPEAFGRFYVLMRAYVVAWTLVGVWAVFRITYRLTRGSLGASVTAAACFAVMPAVVN
                     AAHEAKPHLPGTVLMLLSVLAAMRCLDNNRRNWWILACLLAGAAGGMVLSAWPVFATL
                     VMLAWLAPQPKVARLAKAVIGTAIALLTYFATNPYVLINLFRNRDLIWNNLSNTQAMF
                     HLGPPDESLPNALCLIGEGTSIVLALAGLAGLVALAVRDWRMVSSNPPVGPRFVAHWL
                     LAAPAFLALTQFVLFAHGQGGEYARFALFVDVTLCLAAVVALDTFTRYWKLRTICQIA
                     LLLATAVPGAAYVESFLRDSASHHTRIRQAEHLAELQRNGARTLLTDRVPAPYCLPPV
                     NLFDWTIILAPRGQPDAPGQNADVSIRVVDVPPAPGSPSSDYVPFSTDGRPWLGLARM
                     SWANKPFAGRARKPLLTNSAEAPADAP"
     gene            complement(27806..28720)
                     /locus_tag="P5316_04145"
     CDS             complement(27806..28720)
                     /locus_tag="P5316_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HRR84179.1"
                     /translation="MIRDTFKTVRRWGEMVKFSHSVFALPFALLATFLASRAAFQAGD
                     SPQIYPSLWQFLLILGCMVSARSAAMTFNRIVDAAIDARNPRTAARAIPAGIISPSQA
                     LVFLGVAAAAFVLCCAGFWWIERNPYPLMLSLPVLAYLCFYSYTKRFTRWSHFVLGSA
                     IALSPPAAWLAIHPASLGWPALILLTTVTLWIGGFDIIYACQDIAPDQAEGLFSLPAR
                     TGVRPALWIARSAHAVTVVLLVALAPVAGLSWLYGIGVAAVAWLLLIENAMVHPRDLS
                     RVNTAFFTVNGIVSLLLGTLGIADCLLL"
     gene            complement(28717..29310)
                     /locus_tag="P5316_04150"
     CDS             complement(28717..29310)
                     /locus_tag="P5316_04150"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRR84180.1"
                     /translation="MHTREIILAITGASGGPYALRLLDCLDKAGIQIHLIASPNGRRL
                     LRDECDLRNLEDLSLLGRPCERIRIHDYEDVGGLLASGSHPTDGMVICPCSSNTLGTV
                     ASGRADNLIGRAACVTLKEGRRLIIVHREMPVNGIDLENMLRLQRAGAIICPASPGFY
                     LRPKSIDDLIDFVVARVLDLLHVPHTLNARRQETPSP"
     gene            complement(29329..31251)
                     /locus_tag="P5316_04155"
     CDS             complement(29329..31251)
                     /locus_tag="P5316_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84181.1"
                     /translation="MSLARAWRVLQRAGGRAEEPQAVADVWRWTGPKYRIRAVLFLLT
                     DALLFAGLGCFTFWLRTGYALPFLCDQYWDYLWQSLDPTLDRQATLLEFLLQPIPAQQ
                     VPWMFVIHGLVLASLTAVPILVSMLYRLPLSLVFTAIIAFFATLPWLAITVTFCCMLA
                     RWKRIQFSFRFATALISILPVVLYYLMATRNTPGVDRLAPLELAKLYLPYAIALIAAC
                     VVMAIVLTIARVVNYRPGAIAPLMAILFATPVILFETKVGRDELSYRLLERGFGPGSR
                     EHFLGHADAEEIILRTAKAIRESSPTPQPPIESLVQLVRDDLRLRAALGQTGQSRVAR
                     TLTADWYAQKDMAINECRRFLRHFPYSEYVPSVLYLQGRATDLRLNREFTYLQDSLIL
                     AYNEDFPNTASADVWRKLVEMAPDSAPGCVAMYRLAVIETRRGRVQEALDLLSRLIAG
                     FGKRKITETAPTETSGWRNFLAKRPPANTLGIDVTATVQNARKLEDLLTKNRLKELEP
                     AYDYPILRRLFSIDPHHPEYARNLRELLAATSTDPQDPTNLLRDNIELLLALTNRSRS
                     RRIEELRGLVDRFAKDKRCDALPRARFELAAAYQTDNLLDEARTLYQTIQMEHPDSPW
                     ATEANWRLAVMGQRTE"
     gene            complement(31366..>31956)
                     /locus_tag="P5316_04160"
     CDS             complement(31366..>31956)
                     /locus_tag="P5316_04160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRR84182.1"
                     /translation="EMVRLAAVRPDDVLLDLACGTGDVARAFASASPRPAGIIAVDFS
                     LAMLKLAVPRHVNDLTWVQADALHLPLAPAGVTIVTCAFGIRNFQDLGAALREIHRVL
                     TPGGRAVILEFSIPTVAIFRHVYRLYAGHVLPALAWLIGHDRTGAYRYLHKSVLSFPG
                     REEIVSSLRAAGFADVVVHPLSWGVVTIYLASKTST"
BASE COUNT         5900 a         8850 c        11094 g         6112 t
ORIGIN      
        1 agccgatttc gttctcggtg aggcaaagcg gtgaaggcgg catcctcgcc tttaccggca
       61 aggcccgcgg cgacgagagc gccatcggcg agccgttcat tgtggattac gacgtgttct
      121 accggggcaa cctggttgcg gcggcgctgg atctatcgaa ggcggggttt gcagacaagt
      181 cctacatcaa ggtcgagccc gtcaatgacc ttgcctatcc ggattggaga gggcggccga
      241 tgcaggttga gttggctccc gaggggaagt tgaccgtgct ggtcggcagc ggcgagtatg
      301 ggggccagaa ggagcagcga atacgctttg ctctgaagat cacgcctctg gcggctggcg
      361 aggtacagtt gacaggggtg cgggtgatcc tgcgtcttca ggtgagtatc cgccggcaga
      421 atgtgggatt cgttttccgg agcgacacga cgaacgatta tgaccgcgtg gcggatctgc
      481 cgcctcaccg tgtggttgat ccccggggca agaccgcccc ggccgggtat cgcggagcgg
      541 tcggcgcgaa cttcgcattc gttacttcgt tggaccgtac cacggccgcg gcgatgtcgc
      601 ctttgacgct gacaatgaaa atcacgggcg agagtgtcgg cccgcagttc cagccgcctg
      661 tcctgaccga ggtggcggag ctgacccgtg atttcgacgt gtcgccgacc gtttcgggcg
      721 gcgagatcga aggcaacacc attaccttca cacagatcat ccgtccgcgg agcgaacggg
      781 ttagcgagct tcccgccctg ccgctggttt tctacaacta tcagaaacag caatatgaga
      841 cggtctacag tctgccgatt cccattgagg ttcgcgcggg gtcgctggtc ggggcgcagg
      901 ccatgcaggt ccggagcgga acgcaaccgg cctgggaggc ggcggtcgca agtggcacgg
      961 cgagggagga tgagccggcg gcggcgctgg gtgcgaattt cgattctctc ggcagtttgg
     1021 ttcgcgctga accgctgggt ctgaacggct tgttgctggt attggcaggt gggcctgcga
     1081 tcgtgctact ggtgcgcgtg ggcatcgggg tctttcagcg gcggcgtccg atgagcgagg
     1141 ttcgccgggc gcaggcgagg ttgttgacct cgctggatcg tttaggcgag gcggagcggt
     1201 ttcattttga gctggccgag atcgtgcagg ccttcttgcg gctgacgttt ggcctcccgc
     1261 ccggggagat ttcagccgat gacctggggc ggttgctcga cgagcgacgg gcggggagcg
     1321 agctgcgaac ggcagctttg agcctgctga ccgagtgcga cgccggacga tttgcggtcg
     1381 gagcggtgga tcaggttgag aaggagcggt tgattcgtca ggcccgcgag gtgctgacca
     1441 aaaccgagcg gttggcgaag aattgagagt gaggagttgg gaattgcgaa tgcggctgct
     1501 tgggccagtg tgccattgct ccgcggacgg tgaagaagac tcgatacgat gacgtggatc
     1561 tcccgatcat gtttcgtgtt gcttgttgcc ttcgcggcgg caagcggcgg tgcgcgcgca
     1621 cggggggcat ccgcggagga gcgattcgcc gaagcccagg cactgttcga ggccgcgcag
     1681 aagcgcctgg cagaggagaa gtctgaccct gtcgaagccc gacggatgtt tcacgaagcc
     1741 gccggccgtt tcgccggcat tgccgccgaa ggcgtggcga gcgtcaatct ctgcgtgaac
     1801 accgggaatg cttatcactt cgcgggcgat aacgcccggg cgctgttgtg gtacctgcga
     1861 gcagagaagc tggccaacac gcccgaagta cgctccggtt tggcgacgct tcgcaggatc
     1921 atcaatgccg agccgaggcc tccggagcgg acgtccatcg gtcgggtgct gatgttctgg
     1981 cactatgatt tgtctcgccg gacgaagcag gtcctgatgc tggcaaccta cccgatcggc
     2041 tgcggtctgc tgctggcggg gttgatgagt cgccgccgtg tgctgttgcg cctggccgtc
     2101 accttcatgc tgatgggagg cgtcgtgggc gtttccgacc ttatgacggc gatgatcccg
     2161 ccggcaccat gggcagtagt cgtggagcca ggcgaaggtc gttccggtaa cgggtcggcc
     2221 tattcggtga ttgtcccgtc cgtccgtatg ggccaggagg tgcgtatcat cgaaaggcgg
     2281 ccggactggg tgcgggttga gatgccctcg ggggtgcgct gctggttgcc ggcggagact
     2341 tgtgaggcgg tctgagcgag cggttcggcg aagcccggcg tctcgatttg atgcggacat
     2401 caggttggct tataagtggg caggcccgtg ggtcgggcaa aggagcaaca gcgatcatgc
     2461 aattgttcaa gcagcagact cgacgcgact ttctgaggga ctcgctggcc gctggggcgg
     2521 cggtgagtgt gatgagcatg aacgagttga gaggtgccga gacggcggcc aagccgcgat
     2581 ggatcattgc ctgccgggac gcccacctgc ccgagaccgg tgagccggat gcctggtcgg
     2641 cgatgaaggt catggggatt gaaggcgtgg aagtgtcggt cgacctggcg ggccagtgtt
     2701 cgcacctgca cgggcgcgag aaaccctact ccatcgccaa gcctgatgat gtcaaggttc
     2761 tgggcgacga tctcaagaag cacgacctga agatctcggc gttctgcctg cataatcatt
     2821 tcgacgagaa cctcgaaggg gagcttgcgt gcgtgaagat gaccgttcgc gcggccaggg
     2881 aactgggcgt gccggccgtg cggctggaca ttgtgccgcg gaagatcaag gacgagaagg
     2941 agtttcttga gttcgcgatc aagaccggca agcaggttgt cgaagtgtcc aaagagacgg
     3001 atgtgcgctt cggcgtggaa aaccatgggg gtacgacgaa caaacctgag tttctcaagg
     3061 cgatgttcga gggcgtggac tcaaagcact tcggcctgac gctggacacg gccaatttct
     3121 actggttcgg ccacccgctg acgaagctgt acggttattg ggaggagttg gccccatata
     3181 tctgtcatac gcactgcaag agcatcaagt accccgaaag cgaacgcgag aagcagcgac
     3241 cgatgggctg ggaatacggc aagtactgct gccctgttta tgagggcgac atcgacttca
     3301 agaggcttgc cgccatcctg cgcaaggcgg cctacaaggg cgatctgtgc gtcgagaatg
     3361 agtcgctcgg ccggttcccc aaggagcagc gcagggagat cctgatcaag gaggccgcgt
     3421 ttctccgcaa gatcgccgaa ggggcgtaaa gttgcgcgct gatctcgggt gccatgccca
     3481 cggttttgcg tgggcatgcg atgctcgtat gctcggcccg gaatccttac ccggcccgca
     3541 tcgcctgccg cttgatcgga gtgtgcgatg aacgcccgac gactgatccg aggacttgtc
     3601 gccgcccttt gcctgcttct gagtttcgcg ttctgccatg tcaccttgtc cagcggcaat
     3661 tacaggtcca gttacagaga tgcggcgctg ttggcgttcg tggcgattgt catcttgggg
     3721 ctcagtcttg cccggcgccc cagcaaggac agatgggtcc ccgaagcggc tctcctggcg
     3781 gccggatcgt actgtcttgt gtattcgctc tggcggtacc tgaccagcca ctgagatcca
     3841 tgggggcatc cagaagagta ctcctgcgca gtggctgaac gggcagattt cgggcagtga
     3901 cgttcgctct cctggatgtg ttattcaccg ctcggcccgg aatcctttcc gggcccgtct
     3961 gcaaagcaag gcgtttggga tgattgcctc attcatcatt cctgatcgcg atcatttccg
     4021 ccatctgcac ccattgctcg acgctgagtt gttcggggcg ctgcgatagg tcccatcggc
     4081 cgtcggactc gacgaggtgg aaatgctcat cggatagaac gcggctgagc gtccagccga
     4141 gcatcttgcg gcggtggttg aaacagtggt ggacgatggt ggtgagttgt tctcgcacaa
     4201 gcggagatag cctgtcgcct gcgcgcagat tgattcgcag catcgccgaa tccacctgtg
     4261 gctccggcca gaacgcgctg ggcggcacgc gggtgattcg ctcggtgcgg gccgtggcct
     4321 gcgcgaacac gctgatgggt ccgtagtcct tgttgcccgg ggaggcggtg aggcgatcgg
     4381 ccacctcggc ctgaacggtg aagcacatgg gcgagacggt cagttcgccc atcagtagat
     4441 ccatcagcag tggcgtggcc acctggtatg gcaggttggc caccagcatg attcggccgt
     4501 ggagtttcgc ttggtgcctg gtcagggcgt cgagcacgcc gggatcgatg gtcgatttgt
     4561 tggccaggat gtcgccgtgg atcagcgtca tattgcctgc ggcggcgaag cggtttctgc
     4621 agatctcgtg caggccacga tcgatctcga ccgcgaccac gtgcccggcc tgctcgacga
     4681 ggtagtcggt cagggttccg gtgcctggtc ccacttccag gacgacatcg cgacgagtga
     4741 ttccggcggc ctcgacgagt ttgtggagca ggttgccgtc gatgaggaag tgctggccgt
     4801 gttgtcgcag cggccgtaag cccgccgctt cgagcatcgc acggatctcg gttttggtct
     4861 gggccatagc gtatggttat cacgccgtgc gagagaatgt caaaggttgg atggcgtgcc
     4921 cgattttctc tacttggccg ggcggacggt gatctcgtcc acgtacagcc ggttcgtctc
     4981 cagcgcccac cagttgagga agtggacgcc gatggtcaca tgatccttct tcggcgtgaa
     5041 gtgtagtgac accggcagcc agcgatggtg ggtggcgaac cactgcgtgc agttggcctg
     5101 gtcgatggtg tcgaagcttt ccagggtccg gccgtcgtct tcatcgaccg cgaggcgaat
     5161 tcggctgtcg cgaccccagc cgctaccgcg gtcgccggtc agcaggaacg cggagaagac
     5221 atattcgcgc cccggctcga cggcgatgcg ttgatagatg acctcttgcc tccagtcttc
     5281 gcgaggttcg acccacgtgg ggtcttcggg cccgcggtaa gtccatccgt agaggcgctt
     5341 tccgctgtgc gggttgcagc cctccacgcc ggttgtcgcg tcgccgaacg gcttggcgtc
     5401 gggcacgtag gtctcgggcg tcttggctcg cccgctgccg gctttgcgcg tccagccggt
     5461 gacgccttct tcgaaaccgg gattcaccac cgccacgcaa tcatcgcaag caggcggttc
     5521 aacgatgatc ggcggcttgt cgaaacgcgg gcggtcctcg ccggcgggcg gggtcatgaa
     5581 gctgtaaatc cgtgagtacg ttctgcgcga ggatttcttg taggtgtgtg atgagacgcg
     5641 gtacatgtat gccgtgccgg gggtcaggcc gttgatcgtg acttcatgtt gcctggatcg
     5701 tttctgctca tcaccggcct gctcaatcag atgcacggtt gtgccgtaat gtaccaagct
     5761 gtcgcccggc gtaccggttt cccaggcgat gacagcggac gtgtcggtca cgcgactgac
     5821 ccgcacgttg accggataga tgattccggc gggttcctct tcgccgatga cggagaagag
     5881 ctggtcgaac tgttcgggca cgagtttgcc gtcgcgatag gccctgccct gcttgtagac
     5941 gttgctggag aggtatgaga agaatggttc gccgtcaacg cggcggtact ggaggtagta
     6001 ggtttttccg ggagtgactt gtacggcatc agggaaccag accgcatcgg cgccccaatt
     6061 agtgaccatt ttgaccgtgc aggccggtcc gacctgcggc ccgcccggtc cgtcctcgtg
     6121 gatggagaag aggaattcct gcacccccgc gccgccccac ccgcagttgg cccctgcttt
     6181 caggatattg ggggtcgtgg ctacaaaggt ttgccccgcg acggtgcagg tgccggagcc
     6241 aggaccttcc ttgatgaagt gctgatcgtc gtctgcgcct gcgacctgga tcaggcccgg
     6301 ggtcgcctcg tcgatgtgaa ttcccaggtc gctttccggg tggggcacgc cgtcgtagta
     6361 ggcaatgcca tcgggataga cgtcgccggt gcggggcatg ccgtggttcc agggtttgcc
     6421 gtccgcggcg gccagttcga tggcgtattg tctgccgggc gtggtcttga attgctcggc
     6481 gggcagcgga gacccgcaga cccaggtcat tgattcgcgt gtttcgaagg ggccggcgat
     6541 gcgttttccg gcaggcgaat cctcatagag tgataccagc agcttgctat caccggatga
     6601 tcgccagagc gagaagcccg tcacggccgt gccgcgtgcg acgaagctct gagcgaatct
     6661 cacgcgagcc ggtccccaca gctccatttc gagcgatagc ttgggctgat cggcctgatc
     6721 gatcaccgta gtccttccct cgacgacttc gacgcaaggt gccagagctc ctgacacatc
     6781 aagcatgtaa agaccgggct ccaggccgct gaattcatac aggccgctgg gtccggtaat
     6841 ggtccagccg accagccggc cgtctcgtga ataagccgcc gcgctgcgtt cgatgccgcc
     6901 ggcgttgccc ttgatcttgc ccgccgccaa agcatcggag accgttccga aagccatgag
     6961 aaccgccgcg atgatcgata cgactcgtgt gccgcgcatc atttggccct cacctgctcg
     7021 agaaacgctc catccacata caggctgctg ctgggcaggt tccatcgttg ttcgaggtca
     7081 aagcccacga tcacggtgtc ggccgcggcg gtgaactcca cggatgcacg tcgccactgc
     7141 ccttccgtcg cgtaccactg ggacgtttcg atgtgatcgt tgtcatgcca ttggatgccg
     7201 ccgccgggaa gcacggtcag ccgcaccttg acgtcgctgc tgcgtccgcc atcatgatcg
     7261 gtgtacacgg atccgctgaa gcggtaagaa aggcctttca caactttcac cgcttgatac
     7321 acgaatgagc gggagccctc acctttgtcc gcgttggtcc agccgaacat gcgcgtgccc
     7381 cacatcggag tcacccctgc gtcacagccg acgacggcgc cgcagggacc atccagcagc
     7441 catccttcta ttccactctc gaaagacggg tttagcagct tcaccgcgct gccgccgtcc
     7501 cggccgacca gacggacgaa gtcggcgtca ctgatctgtc cggcgacggt tgcgcagagt
     7561 tcccagtctg gggccggagt ggcttcgaag aaagcctgtc cggaggcaaa gcaagcgggt
     7621 tggacggccg cgagaaagat gtcgccgctc aatgactcta tgtggagata caaggtttga
     7681 ccgggttcgg tgggcacctc gtcagggccc cacgggacgg cggcatcctc ggcgacctgg
     7741 accaccttgc tgggtccgat ctggcggccg ccagggcctc cttcgtgaat gctgaacctc
     7801 gcatagacgg gggggccatg cactccggat aacgccgcgg aggcaaaggt gatcgagcgg
     7861 ctcagggcgg tgaaggtttg gccgactgcc cggagacgcc acgtgtgcca cccggcgctc
     7921 atggcgtagt cagtccgcag gccacagttg tcctcagaga cgacgatgcc gaggtcagag
     7981 tcgggtcgag gcgagccgtc gaaccaggcc gagcctccgg catagacatc cccgcggccg
     8041 gccaaccctg gaacccattt ctcgccggcc ggtgcggtca tcaagaaagt gagaacctgg
     8101 ccaggtcgcg tgggcacctc gccaccggac cagttaacgg gctcggtgga ttcgcccccg
     8161 accggtgccg tgatccgcgg cggtccgacg tgcgggcccg ttggtccttc gccgtcgacc
     8221 acttggacgg tcacgcgacg gccgggcccg aaacctttga cggcgaccgc cgtgataccc
     8281 aggcctgtgg cgacaaatga ctgggcgaac gaaggataag gctggaccca ggagtcgcca
     8341 tcgatgagga gtgatctggt gaccttgaga ttgagcactg ccggctgatc agcgggcaca
     8401 tcaaggtcag gaacagaaac gggctggtat gtctcgcccg gtggcaggca aatggcatag
     8461 cgacccggtg gcagatcggc cagcgtgtag gatccgtcgc cctcgctttg cgcggcgatg
     8521 gccacgctgc gtccgtgtgc gagagctcga acgacggtcc gggggatccc cttgccggtg
     8581 tcggcatcga ggatgcggcc cttcacggtt cctgccggtg acgcgcggta agagagaaga
     8641 agcacggaga acgtaactgc tgcgtgagta aggagatatg gttgggtgct catgaccggt
     8701 tcccgcaggc gttgggtttg tttagcgacg gggcgccagc atagcagagc cgtatcagcg
     8761 gcacaagctg acatgtaaag ggggccgcca gggcgggacg ctggttggaa tcaaaatact
     8821 ctttttagat ctcagcctgg acgccgagcc gttcggccaa gcggtcgaaa tcttcgaggc
     8881 tgtagtaatg gatgatgagc ttaccggcgc cctttcgttt cgactcatcg atttccacct
     8941 tggttccgag ttgtcgggtg aaggcttctt cgagggccct tatctggggt cgctttgcgg
     9001 tgttgtctcg gattggtttg gcggccgcct cacgggcgtt gacgcggtcg cgaacgagct
     9061 tttcaagcgc tcggacggag aggccaaggt ttatggcttg tctggcggtg ttgaccaagt
     9121 cggaaggaga tcgtatcgcc aagaggcagc gggcgtgacc catggacaat tgcccggatg
     9181 tgacccaatc cttgacttcc tggggaagat caagcaggcg aatgtagttg gtgacggtgg
     9241 agcggtcttc gccgagtttt gatgcaacct gctcgggggt tagggagaat tcgtcgcagt
     9301 atttcttgta tgcagtggct cggtcaatgg gattaagatc ctcgcggaag atgttttccg
     9361 tgagggctat ttcgagcatg tcttggtcgc tggcctcgcg gacgatggcg gggatttcgg
     9421 aaagaccggc catctgggcg gcccgccagc ggcgttctcc ggcgatgagt tcatatacgt
     9481 ttccgtcttt tgtgcggacg atgacgggct ggatgatgcc attggaggag atagaggccg
     9541 caaggctctt gagttcattg ggagagatct ctttcctggg ctggaagggg ttgggtcgta
     9601 ttgttccaac gctgatggtt accgcaggag gtctgggttt ttcatcgcgg ggctcgttag
     9661 cggcgttttc gagctgggcg gggggttcga atggggtggc aaaggacatc agcgagctga
     9721 ggcctcggcc caggcgtttg ggcgggtttt ttgtcataag tgcctccggt gggctgcgga
     9781 tcatggcccg taattgagat taaagatcta cgcgcgcggg acatggtatt tgtttggcca
     9841 ggacgacgca agtggttgtg gtgggtttcg gggggatgca ttcgattggg ttcgtttcac
     9901 gtggaaccat tgggcgacct gaggccgggc gtgttccacg tggaacggtg ctcggagagg
     9961 tctgctggac cgggagtggt tacacgtaga acaaggctgc ctgagagatg ttggtgaagg
    10021 gtacgtgagg gggtgcaatg gaccagctcc gccagccaac gattcctcct ggcggcgaga
    10081 ggcaagggat cgcaggcggc agagcccgag actctttggt actccaaaga gagtcttcaa
    10141 ctggcaagcc gcacataatt cttccagcgc gccgccaccc tggcgtgccg gcggatggtg
    10201 aaacgagagg aaggagccgc ggggcggtga ggtcagagac tcatttgcat acgaaggccg
    10261 acgaccaagg agtcgggaac ggcgtgcgtg ccgccgggtt ggtgaatcca ctggaaatcg
    10321 ggtgtcaggc tgagccacgg cagcagggcg atgttgtagt agatctcata gacagattcg
    10381 cgatcgccga ggccggcgtt tgaggaacga ttgccgatga ttccttggac gaaacccagg
    10441 ccgaggacat catcgtcgcg gttcgggaag atgccctggt attgagcgcc gatgctccag
    10501 aagtgctcga tgagatggac atcgtcgtgg gcgtatccat atcggaagaa catgcgaatt
    10561 ccctgcgcgt cgtcgtcgcc gttggggtcc tctttccaca gcatctggtc catgttgaag
    10621 gcgaagccga cgtcgtcggt cttgtggcgg agaacgtccg tggtattgat gagcagcggc
    10681 tttggctcgg gtttgtacca gagcatcagg cgataggctc ccggcagctt gccccggagg
    10741 gttttccaga cggggaggag gccgagttca aggatggaga aagtatgaca ctcgtcgtgg
    10801 taaaaggtgt cgaagccggt gtgtcggcca ttggcttgag cgtcggcgat ggcggcgcgg
    10861 aggtagaacc agtcgaaggg ttggatggcg gtcatgatgc cctgagcgta gtcggggaag
    10921 gggatgttgc ccatgttgac cagcgcgggg ttgagaaact gggtcttttc atcgttggcg
    10981 acggcgttga gatcgaagtc gatcatcaga tccatcttgc cgaccttaac gcggaccttg
    11041 tcgtccagga gggactgctg aaaccataat tcgcttacct gcagggcgta atcgccgagg
    11101 taggcatcgg cgttgacttt gaagagattg ccgaccttgt cgatgccgat atcgttgttc
    11161 caactgcttt cggccagcac gtagagaatg gcgcctttcc agaggcccat ctgctcgaag
    11221 tggagggtga gctgggcatc gacgctgccg gatatgtcat ggccgttgtg agtgtcccgg
    11281 ccgccgcggg cattgtgttg gtagatggcg gtcatggaga gcgagaaatc gatccccttg
    11341 ctttcaagcc agttgcgtag accccagcag tcgccggtga ggcgtttgtt gtgcaggaca
    11401 tcaaaggggg ctggggatct ttgctcctgg gcaagacctt cggaaacggg ttgggaggct
    11461 ggaatctggg caagggtgga gcaagccagc agcagagatg cagagacggc ggcgccgaca
    11521 ggtcgggttt tcttcattgc aggtctccgg tcagacgtgg tggtcgcagt tgcgtttgtc
    11581 ctggtagcat catcttccgg cgcctcggct cgcacgtccc agggttggga gtggattccc
    11641 gggcggcccg tttttgtcac ggaccgtggc caaagcgtgt cgtgatttag aaacctgcgt
    11701 agagaccgca ggggtccaag gcgattgtaa gacgggtttg ggagggtttc catcccaagg
    11761 cggaccgcct ttttgcgtgc cgcaacgcag gcgcaatgcc atccagaaac atgcgcgcaa
    11821 aacgcgcacc gggcgccgcg gaagagattg tggtcggctg tgtgagcggc acgtttcatg
    11881 acgagcgttc cggtgagaaa ggggtttgac aaggactggt acgggacctg cgggcggttc
    11941 ttcacgaacg gggtgagatg ggttatatcc gaggggcggc aggtgggtgc ggtcccgaca
    12001 taacgaggag aacaacatgc aacgtctggg aatgatgctt ggcgtgctga cggtgctggc
    12061 cgcggtgggt gtgtgcgggg gcgccacggt tcagaaacgg tattacgctc atgagacggt
    12121 tgaagaccgt cacggagtga tcgcgccctg gtaccagggt cagaacggcc agttggattt
    12181 gcgtgtgcgg atcgcggccg agacgctcaa gcgctaccca tggacggaac ccggcaaggc
    12241 ccccgttcag gtgcccgagt acatctgcag cggggcgtgg aagatcgcgc ccgacggcac
    12301 aatcacaatt ccgccgatca gcgactgggc caacggtgat ctggggcagc gggcggcgta
    12361 tgttctcagc gggctgatcg actactaccg ttacagcgga gacccggcgg cgatcgccca
    12421 catcgcgctg caggcggatt acttgctcga ttactgcctg acgccggatg accatccgtg
    12481 gcctcgcttc ctggtgagcg tgccgacgaa gggcaaacct tacggtcagg ccgatccaca
    12541 cggcttcatt caactggata tcgtggctga ggtcggagcg ccgttgatcc gagcggcgca
    12601 gatgaccggc aaccagcgat ggctcgatgc ggccaagcac tggggcgatc tgctggccga
    12661 gaaacgcgac cgtcgcccgg gcgtgccgcc gtggggccgt tacgccaacg ccgaggatgc
    12721 gccctgggag gatatcgcca ccggcggcat cgtgttcatt ctcgatttct tcgatgagtt
    12781 gatccgcgcg ggttacaccg gcaaagacaa tgcgatcgtg gaggcccgca atgccggtgc
    12841 ggcttacctg cgcgacgtgc tgctgcccga ctggacgggc ggcgacgtct gggggcgcaa
    12901 ctactgggac tggccctgca acgtgcaggt ggagaacgtc accgagttcg ctgttcgcta
    12961 tctgatgaac catccggacg aattccccaa ctggcgcaac gacgcgcgca acatcatgtc
    13021 gttgtttctc aaccgcacgt ccgtggcacc gggttccagc ggcgacgtgt tcagcggagc
    13081 gtgggcatat cccgagtcgt ctgggtgttg cgggcggtcg ctgtggtacg ggccgatgga
    13141 gctggccaac gtgtgggctc agtacggcgt gctggccgac agcgagtggg cccgcgagat
    13201 cgcccggcga aagatcatcc tcgcgacgta tgacgtccat gagaccggcg tggtcgagga
    13261 caacatcgac ggcgggcaga tcgttgccgg cgactggttc aagatcgccc acccgatggc
    13321 actgaagcac tgcctggcgg tgatcgcgtg gatgccggag atcttcgcac cggcgaggga
    13381 gaatcatatc gtgcgaagca cctcggtggt tactgatgtg agttactccg acggctggat
    13441 cgcctacacg acgcacgatg cacctctcaa caccgttgac gtgctccgtc tctcctttcg
    13501 ccccacgcgc atcgaggcgg acgacaaacc cgtgaaggaa ggcgacacgt cacgcggcat
    13561 cgcttttggg gtgaaggaac tgtgtgacgg tgactatctg gtcaacgttc gccatgatgg
    13621 cgccacgcga atcgtgatca aaggggacga tccgcaggag acgatcggcc acgagtcgtt
    13681 ctcctacacg ggcgactgga ccagagccga aggcgtcgac acggcgctgc aacgcactat
    13741 ccggatcgat cgctactcat ccgcagcggg agccacggcc acgtgcagtt tcaaaggcaa
    13801 tcaggttcga ctcatcggaa tggcgggccc agatggcggt cttgcggata tctacctgga
    13861 cgatgagaaa caacttgtcg atctggactg ctggtgtccc cagcccagac ggggtgttct
    13921 ctatcatcgt tcgggcttgt cgaatggcga ccacacgatc aggatcgttc ttcgtggaaa
    13981 gggcaatccg cgctccggcg ggaccatggt ggcgccgctc gaagttcagt actccgcggc
    14041 caaggctgac aacgtcttcg gcgaaggcgg cgggccgacc gacgcccagc ggtgggtctt
    14101 tggctacacc ggtcgtgagg actacatcga ctcggccggg aacgcctggc ggccggcgac
    14161 ggagttcgtc attcggtccg gcgacggcgt cgattcggtc aaggccgcat ggtggacgga
    14221 gcgcaggcag atccagatcg acggcacgaa cgatccggag ttgtatcgct acggcgcgca
    14281 cggcaaggat ttctgggcgg attttaccgt cggaccgggc acgtatcatg cctgcctcaa
    14341 gttctgcgaa acgcggcgga ttgatccaaa gcttcgggcg gtcaccattc tgatcaacgg
    14401 ggagcagagg gtcgcagatt tggacattgc cgcgacggcc ggccgagatg ccgcggcgag
    14461 aacactcgca taccccagca gccagccgtt tgtccagcct gcgggcatca atcatgccgt
    14521 ggacctggtc ttcaacgata tccagccgag aaacggcgtc atttctatcc gcctgcgcaa
    14581 cgaccatggc ggcgaggcga tcgtgcaggc gattcaagtc ggtccgggcg acgggggcag
    14641 gggggctgaa ccggtatcgt tgccgccggc gtcacagccg gcgccggccg accggaccgg
    14701 tggtcttctg ctcaacgggg gcttcgagga aggtgccgta ggggatctcg gcagcctggg
    14761 caagagcggc ggcggccacg gatggagcta cgtctttgcc ggaccgacgg tgtcgtacat
    14821 ttatcccgag tcggcataca gcattcaccc agagtgggga ctgccgaagt tccattcggg
    14881 gaaggaggcc ctgcggacac acaccgacgg tcacggccac acggtggtct atcaggacgt
    14941 cagcgttgtc gcggagcgca agtacacggc ctccgtgtgg gttcgcaccg acgacctgca
    15001 cggcgagggg ttcggcaaag acgccggcga ttcggccgga ctgatcattc aggaaatcga
    15061 caagaagggc aaggtgacca gccacccgaa acaggcggtt cgaaaagcag gcgatttcca
    15121 gaaggtgtcg tttaccttca ccaccgacaa gagaaccagg accgtgcggt tcatcctcga
    15181 cacggtgatc gcatgcccat acgaccaggg ccacgtcacc tatgacgatt gcgagttggt
    15241 gcgcgcggaa taggagacgt tgcgcgaagc ctttgctttc gttaccggcc ctcttgtttt
    15301 gggcgggcgt cccaccagcg ggacattagc tgtccgtgcg cttctgaatg agccggtctt
    15361 gcggcgcgtg cttgcctgag cccggtctta aggcggaccg caaccagggg gctccgtgct
    15421 tcagaagcgt gcgcgcagaa tgcgcgccag ttacgaggga agagactgag gttcgggtcg
    15481 ccgtccggcg cgccttcaac acgaggtcag tttgcgttac aatcccgcgg cgtcgcccgg
    15541 gctaatcggc ggccatcatg attggcaaga acaaggtgca gacgagagga gcggtacaat
    15601 gcccagtttg aaaggcacgc aaaccgagaa gaacattttg actgctttcg ctggcgagtc
    15661 tcaggctcgc aaccgttaca cctacgcggc gaccaaagcc cgtgaggaag gttacgtgca
    15721 gattgcggac atcttttccg agacggcgga ccaggagcga gaacatgcga agcgtttctt
    15781 caggtttctt gaaggcggcg aggccgaagt cagggcatgt tttcccgcag gggtgatcgg
    15841 taccacggcc gagaacctga agcaggccgc cgaaggtgag ctttatgaat ggggcagcat
    15901 gtatcccgat ttcgccaaga ccgcgcggga ggagggtttt gaagccgtgg cgaaggtgtt
    15961 tgaggccatc gcggttgccg agaagcagca cgaaaagcgc tatcgagggc tgctggccaa
    16021 cgttgaggcg ggtacggtct tcaagaagag caagaaggtc gtctggcggt gtcggaactg
    16081 cgggtacatt cacgagggcg aggaggcgcc ggccgtttgc ccggcgtgtg cgcacccccg
    16141 ggcccatttt gaggttctgg ccgagaactg gtgagcccgg cagtctgtcg gggggctgcc
    16201 tggtgaggtt ccgcttcggt tgaacgcggt cgaaatcgaa ccgtggggaa gccgcgtttt
    16261 ggggcgctcc atctgtcaac gggcgcggat tgccgcgagt gcattcatcg gctcgttttg
    16321 aaaccgcacg gtctcgcggc atacaagagc atttttcaca gccctctatg gctttcggcc
    16381 gtggtcggac ctataccaag tttatgggcc gtccaatcta ccctgcgcac gaatccttgc
    16441 gctcgccggc gggtcatctg tagcggtgat cggatccccg cgacaccgcc gcctccgcaa
    16501 ggcgttgctg cgggacggtg gcgtcggatg ggacaggtta aggccaattc agagactcgt
    16561 tttgtcggtc ttccgatcag tccggggttg gcgctttcgc aggtgtgttt gttcaacgac
    16621 cgccggcatg aaggcctgaa ggtctaccga attgccgcgg acggcgttcg gtacgagcaa
    16681 gcccgcctgc gcaaggcgat cgaactggcg gatcgggggt tgcgtgatct ctccgcccgc
    16741 gtggccgagc ggatcggtgc acccgaagca gccatctttg atgtccagcg gacgattctg
    16801 tccgatgcgg gtctgtcgcg agaaatcgag gcgatgatcg cgaaccatcg actgaatgcc
    16861 gaggcggcgg tcgcgcgcgt gctcgattcg tatgaaaccc gcctgcgcga gttggacgat
    16921 ccttacctgc gggagcgggc caccgacatc ggcgagattc gtcgccggct gcttgacacc
    16981 ctgggcaaca tgggttcgtc gctgcgctgc tggacgcaag aacactgcca gcgaggcacc
    17041 aaccggatca tcgtggccga cgaactgacg cccagcctga ccgtcgaatt ggacgccgag
    17101 cacatcatgg gcttcgtgag cgagcgagga ggtccgactt cacatgcggc gatcctggcg
    17161 cgggcgctgg gtatcccggc ggtcagcgga atcaaaggcg cgcgcgagct gctgacttgc
    17221 ggtaccgagc tgttgcttga tggaaacacc ggtgaagtga tcatctggcc ggacgagcag
    17281 acgctggccc ggcgccattg tgtgcagaac agccggccgg ttccgcctca gccggtcgct
    17341 cccgttcagg gaataacggt catggccaat atcagccggg ccgacgaggt ggtcgatgct
    17401 ctcagcatgc aggccgaggg catcggtctg taccgcacgg agttcgaatt tctcgccgcc
    17461 gagcgcctgc ttgacgaaga ggaacaggca gcccgctacg cctcggtcgt gcgggccatg
    17521 gccggccggc ccgtgtactt ccgattgctg gatataggag gcgacaagga ggccccgttc
    17581 ttcgaccttc cgaaggagga caatccgtat ctgggtttcc gcggcagtcg tctgttgctc
    17641 ggcaggccgg atctgctggg gccgcaggct cgggctctgg ccagggcgtc ggttcacggg
    17701 cctgtgcacg tgatttaccc gatgattgtc gaggtcgagc agttcgcgag gttgcgacgg
    17761 ctgttcgtgg aggcgattgc cgacatttcc gccggcccca tctttcatgg cgtgatgttc
    17821 gaggtgcccg cggcctgcct gcaagcccgg gaggtgcttg aggcggcgga tttcggaagc
    17881 atcggcacca acgacctgat ccagtacctg tttgccgcgg atcgtaacaa cgaacgggtc
    17941 gcgtatgact gctcgcctga ccggccggtg ttctggtcgc tgatccgggg gattgtcgaa
    18001 gcggccgaac agaccggacg gccgttgtcg gtgtgcggcg aacttgccgg cgatccacgg
    18061 tacctgtcga gattacggga aatcggcatc cggactgtga gcgtcgcccc gcgggcgatc
    18121 ccggctcttc gccgatcgct cctccagcct tacgcttgac gggcagtggt ccccggcttg
    18181 aacttccgct tgtcggggag gatactatcc aggtcgcgca tcggcggtga gcccggcgtc
    18241 gagcacttgt ccgcctgcct gctgttgacc gactgacggg ggtcggtcaa gggcccgtga
    18301 gtatcagtcg tggcgagcaa cgagatccgt cttctggttc tggatgttga tggtgttctg
    18361 acggacggaa cgattttcat tgatgatgat ggtcgccaga gtcgcggctt tcacatccag
    18421 gacggattgg gtgtgagtct ctggcggtcg gtcggtcggc gggttgcgat tctgacttcg
    18481 aagcgttcgc ctggggtgga ggcccgggcg cggatgctgg gcattgagtt gatcgagcag
    18541 ggagcggagg acaaggtgcc gggactggag cggctggtgt cgcgagcggg cgtatccctg
    18601 gaggaaacgg cgtatgcggg ggatgatctg ttggatgtgg cggtcatgcg gcgggtggga
    18661 tacccgattg ccgtggcgaa cgccgttgag gaagtcaaag agattgccgc gtatgtgacc
    18721 tcgcggagcg gcggacaggg ggcggtgcgg gaggcgatcg agcatctgct caagcgtgac
    18781 ggtcagtggt acgcggcgtt caaggcgatt ggggcggacc gctgaggagg ccgaggtgtc
    18841 ggcccggaga accgcagtgt tcaagaaatt catgctcgtg gtggcaagca tggcggtcat
    18901 tgccgtcgcc tatgtcattt atcaatggca gggtgaggtt gtcccggttg tggcaccccg
    18961 cgaattggtc cggttgcctc gtccggttcc acggactgcg acgcagccgg acggggctgg
    19021 ggagccggcc atccctatcg gtccgaactg gatcaccgcc ggcgatgccc cgcagctgcg
    19081 tgtttatgac aagcagggca aagcgaggat tgtctttcgg gcgacgcact ggagcccgat
    19141 cagcgacagc gaatttgatc ttctcgaggc gacggtgcgg ctggcactgc cgggcggcca
    19201 gttggtttac atctgggctg acgacgggcg cataaccgtg ttgcagggca aggacaacaa
    19261 tccggtagcc aagcgtggac ggttgcaggg caatgttcgc ctgttcattg atctgacgga
    19321 tgaggcgtgg cgtgagcagc accctgatcg ggccgacctg gatcaacatg cggagtcggt
    19381 tgtgaagctt tggcttgacc gggtgcaatt cgatctggat ctaagcctgc tggaaagcga
    19441 cgggccgatt gtgctccaga gcgcgctggg cgacatcgag ggcaagggcc tgaagatcag
    19501 ttggaacgaa cgcgacaggc ggatccagca attgacgatt gcggaagggc ggcgggcggt
    19561 gcttcgcagc tctgaccttg cgggcacgtc gtcgctgaga tcgaaggaac cacggccgga
    19621 gagggcttcc gcggcgcgcg atgcgggctt ggagaaacgg agcgaaccgg cgactcgggc
    19681 cgcatccgcc ccggcgatcg cccggattat gccgccggcg aagcccggcg ggttgccgta
    19741 tattgagttg ggggatcggc gcgaacgtac cgccggacca cgggtcgaaa cgtatcggct
    19801 ggtgtttcac gatcatgtgg cggcggtgca gaaggagcat ggccggcgta tcggcgatct
    19861 gacgaatgcg gactctctgc agcttcttct tgacttcaac cagtcacagc gcggtggcgt
    19921 agggggccag gccagtcgac ctacgggcga gacgcccgcg accgcaccag cctcggcgcc
    19981 ggccctggcg caggcaggtg aggcgatcga gcttacgtgg acggggcctc tggaagtcat
    20041 cccggtgacg ggtgatgcgg aggcttcttc ggcggtcaac cggatgcagt tgatcattcg
    20101 cggaccggcg gtgcagttcc acagtcggga tggcggctcg ggtgaattcc ccgagctgga
    20161 ataccatgac gagacacaga gagtctggct gcgcggatcg aaggacaagc cggtggcgct
    20221 ggagccaagc gaagggcggc atctggtggc tcatggaacg gtatcggcgg acctgaggaa
    20281 gcgcacggcg cgtgtggatg gtcccggtcg gttcacggat cgaagtgagg cggggcctgg
    20341 gccggctgcg gttgttggcg ggcggttgga gacgagcgat tttcgaaacg tcgagattgc
    20401 ctgggagggc tcggtagacc tcgagttcgc catggatgag caggccgcgg ccagcgcgcc
    20461 ggcaacggtg aacgtgtcgc cgatacctca gggtgcctat ctcaagcatg cggttctgag
    20521 cggtcaagcg gttttctccg atgaccgcgg ctcgattcgg gcggacagga tagacgttgt
    20581 ttgcggcgcg ccgcgcaaga acggggcttc gagcgatccg acgagcagca tggtggtcga
    20641 acggatgaac gcgatcggtc atgtggtgat gaccgttgtc aaccaggacg agacggtgga
    20701 tacggtcgag tgcgagcacc tcgaagtcga attcggtatg gctgaggccg gcaagtcttt
    20761 tccgaggcag gcgcgggcca tcggttccgt ggttgcccgc cgggcgggcc gggcggcaag
    20821 gtgggggccc aggtcggtgc gggatgtggt gctccgggag atctctgccc aggacggctt
    20881 gctgctcgat atggagtcgg tgaccttctc gctttcagcg gacgagattg cccgcaagga
    20941 agccgaggcc cgagcacggg ccaaggaaca gggtatcgtg gaagggtcgg aagagtggaa
    21001 gaagcatgag cagcggcttc gcaacagctt gcgggttcgc agcaagacaa ttgcacgccg
    21061 tttgcgagct ggagggggcg tgacggtcct cgacctgaag caggaactcg atttggcggc
    21121 ggactcgatt gattgtgagc ttgggctagg ccgcgaggag atccgcaaag cgctcatcac
    21181 gggcagcgcg gatcgcccgg cgcgggtggt gctcggtggt ttcttcattc gcggcccgcg
    21241 gatcgaggtg gacgcggaaa cccagtcggc cgaagtaccc ggcgccggta cactgcgttt
    21301 catgacccgg caggatcttg acggccgggc ggtggacaag ccgattcccg tggtgctgtc
    21361 atgggacaag catatgtcgc tggccgggga ccgcaacacc ggccggattg tgggcaaagt
    21421 ccgggccgtt tctgagaccg ttattcttga ctgtgatgac gagatgaggc tggagttcga
    21481 agatgtggtt cacccggcgg cggtcagtga accggcccgg acaagtggtc ggtggatctt
    21541 cgggatgctc gccagtaggg taggcccgcc ggccaggcag ccctctgacg cgcgggccgg
    21601 tcggcagctc cgcaagcggc tgaagttgct ggatgccgtc ggcaacgcgg cgatcgaggg
    21661 taaggtaagg caggaaggga ccggtcgcga gacggtcatc gatcagtggc tcattgcctg
    21721 gaacgaatca ctggagcgga tcctgccggc tgatgtcttc aagcctgtga cacggccggc
    21781 cgacaaacca acgcgccttg ccagccgggt tctggtcaaa gggcctcgtg tgatcatccg
    21841 tctggatgaa aaggaaatga gggttgaggg aagcggacac ttgttggtcg aggattaccg
    21901 gttgcggaaa ggccggcgag cgggtcagcg tcccgaagcc gggggttcgc ttatggacag
    21961 cgcctctgtg gcttcgctcg aggggctcgg cccgagccag acgttattca cgtgggtcaa
    22021 ctcgatgtca ttcctgaaca accgaaacac cgcgatcttt gacagcggcg tcgagatgcg
    22081 gcacctctcg ggatcgaaac tcatcgagct ggatcgagtg gcggcggcga ttgccgttac
    22141 cccggcacgt ctcaaggagt atccggggcg cgaagccagc ctgagctgtg atcgcttcac
    22201 ggttgagttt gagcgcgacg cgaagaccgc ggcgggagaa cccgccccgc tttcgcgagc
    22261 gacgcgtctg aagggtttcc gggcactggg ccgccaggtt cgcctggagg agagtggtcg
    22321 cttcgtcgag ggcgttgagg tgagtttcga tggcgcctct cagattgggc gggtgatcgg
    22381 atcgcccgag tttccagccc ggatgcgtta tctcgacgaa cggacagggc ggccgatctt
    22441 ttcgcaggtc gagcagttcc agtgggatca gcgaaccggc atcatcaagg tccgcgagcc
    22501 gcaggtcctt tcaaccggca ggtaagcggg acgagcagcc accacgttgt gatcggattc
    22561 gagtcacgtc gagaaacggg cctttcgtgc atgtatgacc gttccgaaaa ggtaacgcgt
    22621 gttatgtaaa cggaacgtgg cggggcagac aggatgttta tcggccgtca gttgcccgtt
    22681 gaagtgatcg ccgggggtcg taccgtaccg gccaaacggc tttcggcggg caggcgtctc
    22741 aggtttgcgc tgattcaggc tcattcagcg gcaaagacgg acggcgatgt cgctaaggtc
    22801 gggagaatac cccccaaagg gatgtgaata gaagattaat tcgatctatt tttttcgttt
    22861 cagatcttgc cttcccacaa ccgccccgga taatcttatg tagaaggata catattcgtg
    22921 gcaagcatat cagaatgtca cgaactgtag agagagcgag ttataagggt attcgaccgg
    22981 gcagttgacg gcggttgacg cgaagcgggg tttacggctc gtggggctcg gagccccggt
    23041 caggggcagg ccgtgccagt ttgcccgggg tttcggatat gttgccgggg ggcgacggag
    23101 acgtcgcggc atgatgaagt gaaaggcccg ctgcgtgatg gagcacgaca ccctcatgga
    23161 agtgccgagg cacgacgctt ctttgctgag ccgaaggggg tgttggttgg aaaacgagac
    23221 aaaagccgac gagcgattga ccgttcgtga gatccgggga gacgtaacgg cccaggacgt
    23281 gatggaatgg acgcagttgg cgacggccct gatgcggagc aagaagtatc aggaagccgt
    23341 ggagctgctg tatgttgcga tccaaaggga tcctgaggcg ggtaaagcct ggtttgcgct
    23401 ggggctctgc tattacaacc tcggcaggtt gagcgaggcc caggaggctt tggagatcgc
    23461 tcgatgcaac ggcgggccgg cggcgcagat cgatgcttgc ctgcggcgac tggcgatggc
    23521 gatcagggcg gagaaggaga gaagccgccg ccgggcaggc gagaggcaga cggaggtcga
    23581 ccttgcggag gcggatcgtc ggacaaggtg agaaggccgt atcgctttcc gtgtggggaa
    23641 agaacgtgag gctgggatga cgcgtgggcg gcggacatcg cagtcggccg tttgctgacg
    23701 agttcgtcag tggaaggttg cgggaatggc ggagggagga gtgtggggct gccgggcttg
    23761 gcgcagagga agggccggtc agccccactt tttgcactcg gttttgtcgt tgtcaatccg
    23821 cagacggttg ccgaattcat tctgagggct gctcaggggg gagtggccct cgtttgtttt
    23881 gtgagttgcc gatgggcacg accggcagga ggggcggccc agggagtgtc aagcggatca
    23941 tcgcgcgagt cgttgcgctc caagccatcc gacagcccgg tggtgaacgc cgaagggtgc
    24001 gattcaagcc ggattccgga tggtccagcc gtccgttgtg cgaatgactt cgcggtacag
    24061 cgtatcgcgt tcgatgggga tgccgccggc ctcgatgatc aatcgtttga ggtcgtcggc
    24121 ggtcatttcc tggcgccggc tttggccgcc gtggcgatgg gtgatctcgt aatggaccac
    24181 ggtcccgtcg atgtcatcga cgccccagtt gaggctgacc tgggcaagtc tggtcgaggt
    24241 cataacccag aaggctttga cgtgcgggaa gttgtcgagc atcagccggg tgattgccag
    24301 cgtccggagg tcggtcaagc cggtgggttc gggcaggtgg gccagttggc tgccggcggg
    24361 gatgaatgag agcggcacga cgcagttgaa atgtgccggg cgtgcggcca gcgaggcatc
    24421 ctggtgccgg cggagcttga ccaggtgtgc gattcgctcg gcgggcgttt caacgtggcc
    24481 gtagagcatg gtggcattgc tgaaaatgcc catctcatga gcggtgcggt gaacgtcgaa
    24541 ccagtcctgc tcaccgatct tgcttttgaa cgtctcatcg tgtacgcgct ggtcgaagat
    24601 ctcggctccg ccgcccggca ggctgccgag tccggcgtcg cgaagcgcct ccaacacttc
    24661 gcggattgag cgtcgtggcc ggctgattcg cgagaagtga acgatctcca ctgccgtaaa
    24721 ggccttgata tgcaactgcg ggcaggcttg gcgaattcgc cggcacatgt cgacataatg
    24781 ctcaagcggc cagtgcgggt ggagcccgcc gacgatatgc acttcggtgg ccccgcgatc
    24841 cgcggccgcg cgggccattt cgacgatctg gtcgttggag agttcgtagc cgggggcgtc
    24901 agaagggatg caatttccct gttcgagcga accgcgggtt gccggagaac catcctgaga
    24961 ccttctttcc ggccaagggc gatagaagga acagaaacga catcgtaata cacagtagtt
    25021 tgtgtagttg atatgccggt tgatgttgta gtatgtgcgg cgtccatgaa gccgctcgcg
    25081 aacgaggttg gcccattcgc cgagggtgtg gatgtcggcg gattcataca gcagcaggcc
    25141 ctcttgcggc tccagacgtt ggccggcgcg gatcttgtcg gcgatggcct gcaattcggt
    25201 cggcacggaa cctttcatgt tcgactctca cggggcatcc gacaacagcg cctgcaggta
    25261 tgcacgggcg tccgccaggt attgcagccc ctctcttggg ttgggcaacg ccgcgtggat
    25321 caccagccag ccatcgtaac cgctacggcg ggctttttcg atgattgctc gcacgttcat
    25381 ctctccctgg cccagcggta cgattcttcg tccgatgcga tcattcattc gcaggagacc
    25441 cagccgatgg gcgaactcgc cgatgacgtt gcacgggttg acggcggcgc agtggaattg
    25501 tcccgcgtcg agggccaatc gtaatcccgg atggtccatt tccatgaaga gttgtcggaa
    25561 gtcctcgatc tgttcgaggg tgctgtcgag tgcgttgccg acctgcaggg ccataccggc
    25621 gccgctgacc ctgtcgagca gcaggcgcaa cgatctggtg agcatttcga acggctgctg
    25681 tcggcggtcg cctgcccgga cgatcaccgc cggaggacgc agagtcgttg tcgcggcgat
    25741 ctgagccgtc agccccgcca gcaggggttc gatctccatc tcgtcgatcg catcgacggg
    25801 tgagactgac atcgaagaga ggcgaaggtc gttctcttcg agaacagctt tcagcatttc
    25861 cgcgcatgcg gtcgtcgcgg agaactcgcg ttgtactcga ggccagtcga cctcgacggc
    25921 ctcgtagccg atttcacgga tggacggcag ggcctctcgc aacggccgat cgcgcaggca
    25981 gtgggcgtgg cagctaaggc gcatcggccg gtgcctccgc gctattggtc agcaaaggct
    26041 tacgtgcccg gccggcaaac ggcttgttgg cccagctcat cctggcgagg ccgagccacg
    26101 ggcggccgtc ggttgagaac ggtacgtaat cggaggatgg tgatcccggt gcaggcggga
    26161 catcgaccac gcggatgctg acgtcggcat tctgcccggg cgcatcgggt tgcccacggg
    26221 gtgcgaggat gatcgtccaa tcaaagaggt tgaccggtgg caggcaatag ggggctggta
    26281 cgcggtcggt aaggagtgtg cgggcgccgt tgcgctgcag ctcggcgaga tgctcggcct
    26341 ggcggatccg tgtgtggtga gaggcgctgt cccgcaagaa ggattccacg taggcggctc
    26401 cgggaacggc cgtcgccaag agcagcgcga tctggcagat ggtccggagc ttccagtaac
    26461 gcgtgaaggt gtcgagggcc acgacggcgg ccaggcacaa ggtcacgtcg acgaacaaag
    26521 cgaaacgcgc gtattcaccg ccctggccgt gggcgaagag gacaaactgc gttagcgcga
    26581 ggaaggccgg cgccgcgagg agccagtggg cgacaaagcg aggcccgacc ggcgggttgc
    26641 tgctcaccat tcgccaatcg cggaccgcca gggcgacaag gcccgcgagt cccgccagcg
    26701 ccaatacgat ggacgttcct tcgccgatca ggcacaacgc attgggtagt gactcatccg
    26761 gcggcccaag gtgaaacatc gcctgcgtgt tggacaagtt gttccagatg aggtcgcggt
    26821 ttctgaacag gttgatgagc acgtacgggt tggtcgcgaa gtaggtcagc agggcgatcg
    26881 ccgtgccgat cacggccttg gcgagccttg cgaccttcgg ctggggcgca agccatgcca
    26941 gcatgaccag agtcgcaaag actggccatg ctgacaagac cattcccccc gctgccccgg
    27001 caagcaggca agcgagaatc caccagttgc gtctgttatt gtcgaggcag cgcatcgcgg
    27061 ccaggacgga cagcagcatc agcaccgtgc cgggcaggtg cggtttggcc tcatgagccg
    27121 cattgacgac ggcgggcatg accgcgaagc acgcagccgc ggtaacggac gcgccgagcg
    27181 agccgcgggt cagtcggtag gtgatccgga acacggccca gacgcccacg agcgtccacg
    27241 caacgacata agcccgcatc aggacataga atcgtccgaa agcctccggt cggtcaaggt
    27301 agtaggcaag gtctggcgtc aatgcgatga cgcggcatgc tgccgccagc cgcagcaggg
    27361 cgccgaccgg atacacccac agcccgccgt actggtacat tttcgggtca aagtcgcccc
    27421 gtcccggtcg catcgacgcc aaggacatca gggtgatcat ctcatccgga tgatgcgtga
    27481 agaggcgaaa ccggcggaca atctcggcct tctgggcatc ggtctgattc acgggcacgg
    27541 agcggtcggg ttcattgatg ggatcggcgt ccatgtccgc gggccgtaag gcatccaccg
    27601 accggtcgcc ggccaatgcc tgaatgtcga ggcccgacca aacgggatgt cggccgaaga
    27661 ggtatgggtc gatgtctcgt gaaggtaagc cccagttgat acccaggcag cagacaattg
    27721 cgcacccggt caagaggagc gccagagcca gtatctcctg tcgtcgtcgg ttcatgtttc
    27781 aagtgtacac gatgagccgg tggttttata agagcagaca gtccgcgatg ccgagcgtcc
    27841 cgagcaggag gctcacgatg ccattgacgg tgaagaaggc cgtgttgacc cgcgacaagt
    27901 cgcggggatg caccatggcg ttctcgatca gcaggagcca cgcaaccgcc gccacgccga
    27961 tcccgtacag ccagctcagt ccggccaccg gcgcgagggc gaccagcaga accacggtga
    28021 cggcgtgggc ggaccgcgcg atccacaagg ccggtcggac gcctgttcgt gccggcaggc
    28081 tgaaaagccc ctccgcctgg tcgggggcga tgtcctggca ggcgtagatg atatcgaaac
    28141 cgccgatcca aagcgtgacc gtggtgagga ggatgagcgc cggccacccc agggatgccg
    28201 ggtggatggc cagccaggcg gccgggggtg acaaggcgat cgcgctgccg agcacaaagt
    28261 gtgaccagcg ggtgaagcgt ttcgtgtatg agtagaagca caggtacgcc aataccggca
    28321 gggacagcat cagcggatac ggattccttt cgatccacca gaagcccgcg cagcagagaa
    28381 cgaatgcggc agccgcaacg ccgaggaaga cgagtgcctg cgagggggag atgataccgg
    28441 caggaatcgc tcttgcggcc gtgcgagggt tccgggcgtc aatcgccgcg tccacgattc
    28501 ggttgaaggt catggcggcg gatcgggccg agaccatgca tccgaggatc aagaggaact
    28561 gccagagact cgggtagatc tgtggggaat caccggcctg gaaggcggct ctcgacgcca
    28621 ggaaagtcgc cagcagggcg aacggcaggg caaacacgga gtgtgagaac ttgaccatct
    28681 cgccccagcg ccggacggtc ttgaacgtgt cgcgaatcac ggcgaggggg tctcctgccg
    28741 acgggcgttg agcgtatggg ggacatgcag gagatcgagc actcgcgcga caacgaagtc
    28801 gatcaggtca tcgatggact tggggcgaag atagaatccg ggtgaggccg gacagatgat
    28861 ggcgccggca cgctgcagcc ggagcatgtt ctccaggtcg atgccgttga ccggcatctc
    28921 gcggtgcaca atgatcagcc gccggccctc tttgagcgtg acacacgcag cccggccgat
    28981 gagattgtcc gcccgtcccg aagcgacggt acccagcgtg ttgctgctgc acggacagat
    29041 gaccatgccg tcggtgggat gcgacccgct ggccaggaga ccgccgacgt cttcgtaatc
    29101 atgaatacgg attcgttcgc aaggccgtcc gagcagggag agatcctcga ggttgcggag
    29161 gtcgcattcg tcgcgcaaca gtcggcggcc gttcggcgag gcgatcagat gtatttgtat
    29221 tcccgccttg tcgaggcaat ccagcagccg caatgcatag gggccgccgc tcgcacccgt
    29281 aatggccaga atgatttctc ttgtatgcac ggtcttctct ccccgtctct attccgtccg
    29341 ttgtcccatg accgcgagcc gccagttggc ctcggtggcc cagggtgaat ccggatgctc
    29401 catctgaatg gtttgataga gggtacgggc ctcgtcgagc aggttgtcgg tctgataggc
    29461 ggcggcgagt tcgaaacggg cacggggcag ggcgtcgcat cgcttgtcct tggcgaagcg
    29521 atcgaccagc cccctgagtt cttcgattct tcggctgcgc gagcggttcg tcagagccag
    29581 caacagttcg atgttatccc gaaggaggtt ggtcgggtct tgcggatcgg ttgacgtggc
    29641 ggcgaggagt tcgcgaaggt tgcgggcgta ttcaggatga tgggggtcga tgctgaacaa
    29701 ccggcggaga atcggatagt cataagctgg ttcgagttcc ttgagccggt tcttggtcag
    29761 cagatcctcc aacttgcggg cgttctgaac ggttgccgtc acatcgatgc ccagcgtgtt
    29821 ggccgggggc ctcttggcca ggaagttgcg ccatcccgag gtctccgtcg gtgccgtctc
    29881 ggtgatcttt cgcttgccga aacctgcgat caggcggctg agcagatcca atgcctcctg
    29941 cacgcgacct cggcgggtct cgataacggc aagccggtac atggccacgc agccgggcgc
    30001 ggaatcgggg gccatttcca cgagttttcg ccacacatca gccgaagctg tgtttgggaa
    30061 gtcttcgttg taggcgagga tgagcgaatc ctgcagatag gtgaactcgc ggttgagtcg
    30121 cagatcggta gcccggccct gcaggtacag cacgctgggc acgtactcgc tataggggaa
    30181 atggcggaga aatcgccggc actcgttgat cgccatgtct ttctgggcgt accagtcggc
    30241 cgtcagcgtt ctggccacgc gactctggcc ggtctgaccc aaagccgccc gcagtctcag
    30301 atcgtcgcgc acgagctgga cgagcgattc aatcggcggc tggggggtgg gggaagattc
    30361 cctgatggcc ttggcggttc gcaggattat ctcctcggca tcggcgtggc cgagaaagtg
    30421 ttcgcgggaa cccgggccga aacctctctc aagcagccgg tagctcagct cgtcgcgtcc
    30481 gaccttggtt tcgaagagga tcactggtgt ggcaaacaag atggccatca gcggcgcgat
    30541 tgcgcccggt cggtagttta cgactcgggc aatggtcagc acgatcgcca tgaccacgca
    30601 tgcggcgatc agggcgatgg cgtaggggag atacagcttg gcaagttcga gcggggcgag
    30661 gcgatcgacg ccgggggtat ttcgcgtggc catcaggtaa taaaggacga ccggcaagat
    30721 cgagatcaag gccgtggcga atcgaaaaga gaactggatt cgcttccagc gagccagcat
    30781 gcaacagaag gtcaccgtga tagccagcca agggagtgtt gcgaaaaaag cgataatcgc
    30841 ggtgaagacc agggatagcg gcaggcggta gagcatcgag accaggatgg ggacggcggt
    30901 caggcttgcc agaaccagcc cgtgaatcac gaacatccag gggacctgtt gagcgggtat
    30961 tggctgcagc aggaactcga gcagtgtggc ctggcgatcc agggttgggt ctaaggactg
    31021 ccagaggtag tcccagtact gatcgcagag gaacggcaga gcatagccgg ttcgcagcca
    31081 gaaggtgaag cagcccagcc cggcgaacaa cagggcatcg gtcaggagga aaagcaccgc
    31141 gcggatgcgg tacttcgggc cggtccatcg ccagacgtcg gccacagctt gtggctcttc
    31201 ggcgcggccg cctgcccgct ggagcacgcg ccaggcgcga gccaggctca ttcgcggcca
    31261 atctgagttg tcacgggtcg attcgggcaa ttcctgtcaa ccctgcgcat gagacccttg
    31321 ttgatgccgg ccctgcacgt cgacccagga ctcgggtacc ggggctcagg tgcttgtttt
    31381 acttgccagg tagatggtca caacgcccca cgaaagcggg tggacaacga catccgcgaa
    31441 gcccgcagcc ctgaggctgg atacgatttc ctcgcgccct ggaaagctta acaccgactt
    31501 atgcaaatag cgatacgccc cggtgcggtc atggcctatc agccaggcca gcgctggaag
    31561 cacatgtccg gcatagaggc ggtagacgtg acggaagatc gccactgttg ggattgagaa
    31621 ttcgaggatg actgcccgtc ccccgggtgt cagaaccctg tggatttctc gaagggcggc
    31681 accgagatcc tggaaattgc ggatgccgaa ggcgcaagtg acgattgtga cgccggctgg
    31741 ggccaggggc aggtgcaggg catcggcttg cacccaggtc aagtcgttga cgtgacgagg
    31801 aactgcgagt ttcagcatgg ccaaagagaa gtcgacggcg atgatgccgg cgggtcgggg
    31861 gctggccgag gcaaacgcgc gggccacgtc gccggttcca caggccagat cgaggagcac
    31921 gtcgtcgggt cggacggcgg ccagtcgaac catctc
//
LOCUS       DAOTPW010000028        30300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1592_length_30300_cov_5.636337, whole genome shotgun sequence.
ACCESSION   DAOTPW010000028 DAOTPW010000000
VERSION     DAOTPW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 30300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30300
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_30300_cov_5.636337"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..5005)
                     /locus_tag="P5316_04165"
     CDS             complement(176..5005)
                     /locus_tag="P5316_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR84183.1"
                     /translation="MISRLPVAGLVVVVGAFWPAAEEGRRPSGAEAVSIIRERFEEDH
                     GRICSFGSRFAGQPGCEQTAAYIESEMKAAGVSRIWRRPVELVVPVTDHADLAVNDEP
                     PVPIDPLWPNGANPCVTPPEGFTSEAVYIGEGELEALPVDRLSGRIAVMEFNTGDRWQ
                     YAAMHGALAVLFLRPQDTSWTQANGKYMYASVPFPRFYVNDAALSDRLRRDGSLKLTV
                     RSLVRWQRRTVDNLIGYIPAAERGDGQAVAVLHSRYDAACAVPDLAFGAEQAINPAVL
                     LQLVRGLAARPPAYGVLAVFTCGDTFELAASRQLMKSLTDAQAALGGSLAAEEQELGW
                     LREYRQKLAGSDVLGAVAGWSNRRFRNEHVIWQVKYQILQLQAQARRQRKGGEDEAAL
                     ASIDQRRIRMLALQRQLHQDRPDEMDANLLGDLLPDVQRRLDRMIADRKGRLERRRVD
                     MDILRTVGLGAGSASRPILFISLELSSHGRQFAPFAQSHLCEQLVQNRLVGYGEWLRH
                     AAERIDLPAEVRRTYLDDAAEGRRHWQSDLPAPVANGIDAAVSAGCLGMMMATTQDMR
                     LRVDTPMDTQERTDLDRLAPQIAMCGALIDEMLRRPMPLSRSPLSRWFCRWAGIAAMP
                     APGEGRLDLGIPDAVLYAKSYLGAKSFGTRGIGVRDAIVVMTDGEGRFDLDDIFPEKV
                     RWAPLRSEVYRFDALGRVNMAMTRLGTWETPASGHARMADRINLRGEMFECAQIGLFG
                     LHDARYLEDLDKVLPVEVPRGSEPRAWNFAFDEGAFSVFLPTDVDRWQLVFARGDATR
                     RMLVLNADPDEPEGRGFPLEWRQTEAIGLTSARDFALLNTERMARLEGTGVIDPYLRE
                     LHAESRKKLAAAEAALASGDGAAAARETIRALAGQARVYQLTRSATDDAIHAVLLILV
                     CLVPFSYFLERLLVAAAGIYRQIGGFTGILAVMVILITLFHPAFRISLTPVTILLAFV
                     ILFLSVLVIAIVLGRFHTELRHLRRGHSLAESGAGGASAGDFRRFAVLNRAMLLGIAN
                     MRRRKMRTALTLVTLVLLSFLMMSFTGSGMRLHPLRYELTRLDGGQEERSAIMIQRMS
                     CNPMPVWTLDYLGTAYRERAEVLGHWWITYNSLTTQATRTLRLDGPGGRHALLPAVSC
                     IDPREARLLGMDRLAGDDGMATFAASRDAILISSSVAERIAAKQGDRVEMLGSPFTVA
                     GIMDDRGMLSLTGLNGRAYAPLDYSRYLQWDPDPTLLQSKLDLIDSADSTADLTGSAR
                     SEPYHGISPDQFAIIRADRAVELGGSLRSVVIVPHDPRDTMALADDVAVSFRQPVYAN
                     ADGKVVLCAMAELTTLQGFSSVIVPLLIGAAIIFNTMLSSVYERRREIGTMMAVGLAP
                     LHVGALFVAEAAAYGTIGVVGGFVLGQGLGTVAARFDLIPGINLNFSSAAAVWTQVAM
                     MAVVLLSSLWPACKAARIAAPGSESTWKLPVPHGDEMAVLLPFTFHGRDAAPLLVYLR
                     EWLASHTESSLGRFTCGAIELFADAGTGGRGLVAQIWLAPFDLGIMQTVELEIRPGSD
                     PSIHEVRISLRREAGPHSSWVRGNRRFLTELRKRFLLWRSLSPAETERYRGEIAIGA"
     gene            5409..6356
                     /locus_tag="P5316_04170"
     CDS             5409..6356
                     /locus_tag="P5316_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84184.1"
                     /translation="MKKKIRKIILVVVVVLVVLVVAVVLGIDRIAKTGTEVGATYALG
                     VPTTLDSADIGILQGRTELQQLQVNNPEGFDSPHFLKLNRGALTVSLGSLTSDLVEVP
                     EFTLEGIEVNLEKKGAQSNYQVILDNLKKFESQGESEPAKKETKEGKRFVIRETLIKD
                     VKVRVKAAPLGATLIDVTVPIDEIRLRDVGSDSNKGVLVSELTGTILKAIILATAEKG
                     GNLIPKDVLGDLDGALKSLASLKDVGASLSVGVGEAAKEIGAGVQQATQQLQEGTRKA
                     AEEAQKASEQLQEGAKKAADDVAGKIGDLLKKPEDKKPE"
     gene            7055..9805
                     /locus_tag="P5316_04175"
     CDS             7055..9805
                     /locus_tag="P5316_04175"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HRR84185.1"
                     /translation="MSSVKTIDLRTEYLSDPMAIDDPQPRLSWVLDADARGVRQTAYH
                     VLVASSEALLAEDKGDLWDSGRVESDQMAQIAYAGKPLAAEQDVFWKVRVWTQDNQPT
                     AWSRIARWRMGLLTPADWKAKWISLPGPAPVAKGLPARPSPMFRKEFRIGKPVRRAVV
                     TATSLGMYELRINGRRVGDSLLTPEWTDYRKRVEYQTYDVTDLVKTGANAVGATLGDG
                     WYAGRLGISHIVKEGPLRGHYGTKLALLMQMRVYYADGSSDLIITDGTWKVTDDGPIC
                     KACILDGEVYDSRKELKGWDAPGYDDSAWKPAELTGEVKARLVAQPNEPIRVTEELRP
                     VAMTEPQPGVYVVDFGQILSGWCRITVGAPAGTTVTLRHAEVLDSHGMIYRDNLRMKP
                     LGGELGARQEDQFILDGSGSQTFEPHFTYHGFRYVEVTGLPARPAKDFILARAFHSAP
                     PTTGTFECSSPLLNKLMKNIVWTHRDNMHSVPTDCPQRDERMGWMGDMLVFAQPACFN
                     MDMAAFFTKWVVDIRDDQADDGRFPDFAPHPYDPNARFSGVPAWGDAGVIVPWRMYVN
                     YGDTRVLAEQFDACHRWVDWILSKNPDLLWTHDRNNDYGDWLNGDTLQLEGFPKGEAE
                     IPKEVFATAFFQHSTELTAKMARVLGRQAEAEKYGKLAADIRQAFIKAYVSDDGRVKG
                     NTQSAYAIALDFDLVPEDKREAAARHMVERIAAYKNHISTGFHTTVMLMNQLSAAGRS
                     DVAYMLINNRTIPSWGYTIDQGATTIWERWDGYVKGRGFQDPSMNSFCHYAIGSVGEW
                     MYRSILGINPDEQCPGYRHFILKPQIGGGLTWAKGGYDSISGRIVSQWKVNGDAFSWE
                     IVVPANTTATVYVPTADPSTVTESGKPALLADGVSPDGKAAGAAVYKVVSGTYRFASK
                     LK"
     gene            9971..10720
                     /locus_tag="P5316_04180"
     CDS             9971..10720
                     /locus_tag="P5316_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84186.1"
                     /translation="MRNLMLSRLRFRWLIYGALAALLAGSLACEGNLQLLAMLFNTSS
                     LGGTTPGRRANLLVKFVNQTAYRPVFTFGTYDPLNDRPANDPYFPPKFRQFVIDTSGG
                     GTRLEPYSESGVITFSGSGLGDPGGCGRAISLGTEDFIRRIEDDETLLANARPEALRP
                     LCVNDEPKAGIAFFREASAGPSEDPCETKGEIAATAEPMRILQGDPLRNITNDVRYPC
                     DGESIVVITFVEESAGVIRIDVSVEPPAEDE"
     gene            complement(10750..11262)
                     /locus_tag="P5316_04185"
     CDS             complement(10750..11262)
                     /locus_tag="P5316_04185"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HRR84187.1"
                     /translation="MAATPFTDLSSINPNEVVADRKAIYERLPHRFEFMQLDAILRID
                     REAGLAVGLRRVRSDEFWVRGHIPGQPIMPGILMLETAAQMASYLSQEVQPSTKFLAF
                     GGLEDVKFRMAVTPPATMYIVEKIVEVRPRRTICDAQGFVDGKMVFEARIIGMPLAPS
                     APSPNDSQAS"
     gene            complement(11514..11693)
                     /locus_tag="P5316_04190"
     CDS             complement(11514..11693)
                     /locus_tag="P5316_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HRR84188.1"
                     /translation="MLEDYEKLKRLVESAADDVAKAQGGNKAAGTRVRKIMQDVKSAA
                     QELRKKVLEARSAGD"
     gene            complement(11937..12875)
                     /locus_tag="P5316_04195"
     CDS             complement(11937..12875)
                     /locus_tag="P5316_04195"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRR84189.1"
                     /translation="MRVIRGLDFEPMSGVVLCIGNFDGVHRGHQAILQAGRQKARAAG
                     TELVAMTFDPHPLAVLTPERMPAVLTPLPEKLRQLERAGADVIVVADSSAAFLQLSAA
                     DFIEDVIVRRFRPSAVVEGRSFGFGRHRQGDVETLQAAASRHGFEVQIVEPVRIALGG
                     HPDAVISSSLVRQLLTAGPVDQAAICLGRPYALLGTVIHGVGRGAGMGFPTANIDPGG
                     QLVPAEGVYAGFAELAGRRHQAAISIGRTPTFDEHRLVVEAHLLDFAENTYGQSLRIE
                     FLAWLRSQARYETVDDLCQQIAKDVEQTRQICRQNA"
     gene            complement(13066..14163)
                     /locus_tag="P5316_04200"
     CDS             complement(13066..14163)
                     /locus_tag="P5316_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRR84190.1"
                     /translation="MTDQRRNALVGLFVLGGLIAFGIVLVMFGESRFQFLTRGYTITA
                     RFDTVSGVREGTQVFLAGVQIGSVLNVELKNPENPTEGVVAKIDVRGKYEIPVGWKAF
                     VESPLMGQPVINILPPTGRIPIVIPPQRLASLPKDGSGEILGEQVNPLEKILSPQFIS
                     TLEKTTAQIGTLAEALTPAANAVTELLEYRPISLIESPNKPAEITANLFTTVERLHKL
                     LKHLDDVFGDPESQANARAVLANLRQASEDAKLAMTGFKDFSDRLQVAATQASDVLTN
                     VNSTVTLTREHIDTLGKKLVTNIEQLSRLLDQLVATGQTLNEGDGAVPMLLRDPKLYE
                     ELMLTVRRLGEAAQELKVLVKQWQKSGLLGG"
     gene            complement(14160..14948)
                     /locus_tag="P5316_04205"
     CDS             complement(14160..14948)
                     /locus_tag="P5316_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR84191.1"
                     /translation="MKTRDEPIVEFRNVHKRFGRLTVLNGVDLALERGKTTVVIGESG
                     TGKSVLLKHMLGLIHPDEGEVYVGGQRIDKLKEWQLGPVRKRFGFLFQMGALFDSMSA
                     GENVAFPLREHTHLSEREINAVVAKKLALVGLDGIQPKRPAELSGGQRKRVALARAIA
                     LDPEIILYDEPTTGLDPIRADTINDLIIKLQQELGVTSVVVTHDMTSAFKVADRILML
                     SQGRFIADGTADDFRNSTDQRVRQFVTGDADLVIEPVPSGEASK"
     gene            complement(14978..15868)
                     /locus_tag="P5316_04210"
     CDS             complement(14978..15868)
                     /locus_tag="P5316_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR84192.1"
                     /translation="MVDAVPEVTTMSAQDSSATPGCPPETAWLASLGRAVICGLADFG
                     DFWLFAGQTIRYSPLTLLRRRDIQRFLPQCYAIGTLSVPVILITGVFVGMVLAVQAIE
                     QFMSVGLAERMGVIVSLSVVRELGPVLAGVMLAGRVGGALAAELGTMNVTEQLDALRS
                     MGTDPIRVLVSPRFAACVLLTPVLTLYCDLMGVGGGWFICVPLFDIPNSVYWDYTAAA
                     LEKWDVASGLLKSVFFGACIGLISCYKGFNCKPGAEGVGKACTESFVLGFVVILILDF
                     FLAFGLKGLYESLWGFKSVF"
     gene            16282..17973
                     /locus_tag="P5316_04215"
     CDS             16282..17973
                     /locus_tag="P5316_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84193.1"
                     /translation="MATPRTRGRVPSFKERGIGLLILALLVLVVFTFLLSSGLFAGVV
                     QSVSALQRLKGLIGLSEDRLFDIPAGTLASAAPPAEIEIARRLLSAPSQGKEGNGRYA
                     AYAVSAGETVEAVREMEGPAGGSKVIVRAQEQPDALGKEAAGLGTGWIYEHRPLAKDA
                     GFLLRVVDIGHPSNAEKLAAARKPADAKPLGLGRGGWMDEKNGACGFWSGRYYTEVSL
                     AEAAEVGPIPMGELRQIATELAANQLVYGAEVEPAAPTPKETSATPTPAVARVADFAP
                     LPGGQALPPSKIDRFTDNLYEKIDGKEGMFRAFHFVELRFGQYTSARSQEPFDVYIYD
                     MGEPVNAFGVYMGERSPTARAWSLGRDAYVSGTSAYFCKDKYYVNVLGPPEGGDDKLE
                     VSKAIAAAIAATIPDADEPFWVEQYLPAEGRTPNSLKYEATSGLGYDFIHQMFRAEYR
                     VEGGNFQIFVMKTTDAAAAREVFDKYVEATAKYDKVLSREADAQGQKMIGESLGVYSV
                     AFTKGVFFGGVTECEDQALAVRQAEAFRARLPADPEGAIAPSSRPISSPADDGQG"
     gene            17983..19131
                     /locus_tag="P5316_04220"
     CDS             17983..19131
                     /locus_tag="P5316_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR84194.1"
                     /translation="MSETGRHENPTETNASRTWTRRDLIVRGGRTIAVAGLATGAAVY
                     LHDPKGDAGLQPRAPIRLKDYFAKIKPDYPASAPRLSAAYGEPERIADLTHIRALVEA
                     AVAGLDRDLGISRFISRGDVVLLKPNVGFDRDPRLGATTNPLVVQAVIQLCRRARAGR
                     IIVADNPIESPGACFAKSGIAAAAKAEGATVMIYSSAHERAVEIRPGRPEPARNEALG
                     TWPIFWTPLKEADKVIGLPVIKDHNLSSASMTMKNWYGLLSGRRNQFHQAIHDIISDL
                     GYMMKPTLVIADGTRVMIRNGPTGGRLDDVQVGGITGRPTIVAAVDQVACDAWCYQNL
                     LGRDPRDAKAPLQYIELAYAKFGQDPGRIVARHWQDYERQGLIAETKL"
     gene            19207..21048
                     /locus_tag="P5316_04225"
     CDS             19207..21048
                     /locus_tag="P5316_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR84195.1"
                     /translation="MKITTVRIISQIVFMVLFLTFVFVTTFTCLDDAPALKFWVSKYL
                     EIDPLIAIATGLATHALYQGLIWSLAILIPTLLLGRFFCNWICPFGILHQFVGWIFNR
                     RSTPDRIASNRYRPMSQTKYYVLAAMLAAAAFGSLQIGLLDPICLLHRSLTVSVLPAL
                     NMPPVFVEIFGNPKMHQLGWVVGGIVLFLVGMNLVVPRFFCRTLCPLGALLGLVSRFS
                     LWRIDRDPNKCTECGLCDRNCEGACDPGGKLRKSECFVCFNCIDSCPHGALRFAFLPA
                     RLGEVAWPDASRRRAILAAVGGVIFYPFTRLAGKTTRDFSSKAIRPPGSVEEQEFLAR
                     CLKCGQCIRVCPTNVLQPAMLETGLEGMWTPVMNFRMGFCQLNCTACGHVCPTGAIQR
                     LTVERKQGLGEFASAGPIRLGTAHYDLGRCLPWSKNIPCVVCEEVCPTSPKAIHTEQR
                     LMLVRDGKKMVVSATPVTVTISEYPPEGQSVGPRCVLTPNAYRGDPTARYYVQVFHRD
                     GTSETHRIIANDVDTLMIGELDPAGGQLVAGSRLARIPERGDVAAIHIELKVPKIDAE
                     LCVGCGLCEHHCPVVGDRRPVYVTAEGETRSESQADDARNRSFRLVK"
     gene            21509..23281
                     /locus_tag="P5316_04230"
     CDS             21509..23281
                     /locus_tag="P5316_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84196.1"
                     /translation="MRTLAHVTHEAVEKVGGIGAVLQGLLTCNAYRGRDQRTILIGPT
                     FVTEGSADGRLGASGEVLYSSVDGVTQHPVGRLLDQVRRDFHVEIIYGYRRFHDPHTG
                     VRVAAEVVLIDVSRMNPHLVNSFKGRLWETYGIDSSRYESSWDYDMYVRLAEPALAAL
                     KALGAADSDDECVILAHEFMGLPTALAARLDPSGVFRTVFYAHEVAAIRKLVEEHPGH
                     DVTFYNVLSGAMNRGRYLDQVFGSQDHYHRHALVKRSRFCDRIFSVGDYVTKELFFIG
                     PEFADAAIDTVYNGIPAERISVAEKRVAQERMRDYAETLLGDRPDYIFTHVARMAVSK
                     GLWRDLAVLEHLEREFRRIGKTAVLFVLSTEVPPRRPEDVREMERWWHWPLAHREVGS
                     DLSHGEAVYYQGVQVFNARSRMIKAIYVNQFGWEQAVCGSRMPQDMTFMDIRRASDVE
                     FGMSIYEPFGIAQFEPLTFGAICVPSRVCGCVGFLDKITGGATVPNVFVADYCDLGPG
                     RWDEKKLLALDRDQRTQHEYKVAERIARYIIDTLPQTDAQAEEFLRSGYDLAGRMSWD
                     VVAGRYILPAIDAICAKKAGSQVA"
     gene            23458..24417
                     /locus_tag="P5316_04235"
     CDS             23458..24417
                     /locus_tag="P5316_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84197.1"
                     /translation="MMFDSRKTQVRWLTAVVIAGLVAAPGCRPGQPNGSDSGTDELTD
                     EEVITDLREFHPLITDLPPIPLPPELAQRDDDTAKEALDLLDQINSFRNADPLGTTPW
                     DATPNGKSARRLKGDGWQKQCDLLACYYTKKQGNLTVRWTYLFGLPGVWESVLTWDGC
                     DGEHEYDDFTLEHWHMTQDLTLVIQTHYQYPDPPPCGDGQAAIGPGPQFRRIYELIEG
                     GTLYTPGQVIYLDTRRYTIEQWHYHSVIGEYLVFSMLTCTSYPDGRLIFEHYTQRVSE
                     PRLLYLSYKGIWSSDHRYCWTTYREDETVLSCGGDMGCPECEH"
     gene            24668..26767
                     /locus_tag="P5316_04240"
     CDS             24668..26767
                     /locus_tag="P5316_04240"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR84198.1"
                     /translation="MRVGALALGFALLAGCQVPGSYKPPPEYPTFPPLRHAIKAVFGE
                     NGNDHGGDLQGRGMQFVKAFHDLGIDLTRIEFKWAIAEPRRGTYDWSEHDRLIDFLHR
                     HGIEPMLMLYCAPTWAMRGTPEDEQLFIERGEQNLHSVVWPRREYLPDFERFCETAAR
                     RYRGKARLFEFWNEPDGMAGPTVYHDRSGKAVDVRYGGDAKEYTLWLRHMYEAVKRGN
                     PDAVVAAGSLSVHDTRFIEAMYAAGCRDRCDAISLHPYAGDGVNVEWIRQCRAVMTRH
                     GDWAKPVWLSEFGWNLGGEYDQKNGRWPDSAIRQAQIITATAPVIQSLPYITHAFWFT
                     LNDWSTGQTGIDPVGTHRFGIMDLSGRRRPGFAALRDAVLKTPLLPRHAEMPLPQVVP
                     PAGPIDLASESHLKLTLTCRDPSPLFCPDQSEARPQSRALAFEVPGLTRGPHSIPLAY
                     GGSGRGRPDGKVAWTPFDAKADVIVPISPAANRPLLPNTYEAVASFGYGPQTRFPVTL
                     PAPARRADAPPRVDADLADWPSQWPIADGPMKAAFAWSPTHLFFACTVRDARHEQPHQ
                     GRDIWKGDCVQIAFDPLRDAVRGSQYDINDSEYALALTQDGPVIWRFICPPDSYVGPV
                     ADAHVAVQRTDEETRYELAIPWTELSVSDPTPGHLAGVAIAACDWHGDERTVHRFGDG
                     IIGPKQPCRFASIRLTE"
     repeat_region   27208..30213
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=27276..27302
                     /rpt_unit_seq="gtttcaatccacgcccccacggggggg"
BASE COUNT         5817 a         9896 c         9207 g         5380 t
ORIGIN      
        1 caccgcatca tcaaaccggc ggatttacag catgttcaga ccggcgccga caccggcaag
       61 gcggctccct tttgtttaat gaagtcggcc atcaccgaac cccaaaaagg gcaaaagtca
      121 agatgatcgc tcttgtcgta tctgcgatag cgggctagtc ggcccccggg cacaatcacg
      181 cgccgattgc gatctcgccg cggtaccgct cagtctccgc cgggctcagc gaccgccaca
      241 gcaggaaccg cttgcgaagc tcggtgagga aacggcggtt gccgcggacc cacgaactgt
      301 gcggtccggc ctcgcggcgc agcgagatgc gcacctcgtg aatgctcggg tcgctgcccg
      361 gccgtatttc caattcaacc gtctgcatga tgcccaagtc gaacggggcg agccagatct
      421 gggcgacaag cccacgcccg ccggtgcccg cgtcggcaaa caactcaatc gccccgcagg
      481 tgaaccgccc cagcgatgac tcggtatgcg acgccagcca ctcccgcaga tacaccagca
      541 gcggagccgc gtcgcggccg tgaaaggtga acggcagcaa gaccgccatt tcgtcgccat
      601 gcggcaccgg cagcttccag gtactttcgc tgccgggggc ggcgatccga gcggccttgc
      661 aggccggcca cagagaagac agcagcacga ccgccatcat cgccacttgc gtccagaccg
      721 ccgccgcgct ggagaaattc aggttgatgc ctggaatcag gtcgaaccgg gcggccacgg
      781 tgcccagtcc ctggccgaga acaaaaccac ctaccacgcc gatcgtgccg tacgctgcgg
      841 cctcggctac gaacagggcc ccgacatgca gcggagccag cccgaccgcc atcatcgtgc
      901 cgatctctcg ccggcgttcg taaaccgaac tgagcatagt gttgaagatg atggccgccc
      961 cgatcagcag cggcacgatg actgagctga agccttggag cgtggtcaac tcggccatgg
     1021 cgcacaaaac tactttccca tctgcgttgg catacaccgg ctgacgaaag ctgacggcca
     1081 catcatcggc cagagccatt gtgtcccgcg ggtcgtgagg cacgatcacg accgaccgca
     1141 ggctgccacc aagctcgacc gcccggtcgg cccggatgat cgcgaattga tccggcgaga
     1201 tcccgtggta cggttcggac cgcgctgagc cggtcaggtc ggccgttgag tcagcgctgt
     1261 cgatgagatc cagctttgac tgaagcagcg tcggatcagg gtcccactgg aggtagcgag
     1321 agtagtccag cggcgcgtat gcccggccgt tgagaccggt cagcgagagc atcccgcgat
     1381 cgtccatgat gccggcaacg gtgaaaggcg agccgagcat ctcgacgcgg tcaccttgct
     1441 tcgcagcaat cctctcggcc acgctgctgc tgatcagtat cgcgtcccgt gatgcggcaa
     1501 acgtggccat gccgtcgtct cccgcgaggc gatccatacc cagcaagcgg gcctcccgcg
     1561 gatcgatgca cgagaccgcc ggcagcagcg cgtgccgccc gcccggcccg tcgagcctca
     1621 gcgtccgagt ggcctgtgtt gtcaggctgt tgtaggtaat ccaccagtga ccgagcacct
     1681 ctgcccgctc gcgataagcc gtgcccagat agtccagcgt ccacacgggc atggggttgc
     1741 agctcattcg ctgaatcatg atcgcgctgc gctcctcctg gccgccgtcc agacgcgtca
     1801 gctcgtaccg cagcggatga agcctcatgc cgctgccggt gaaactcatc atcagaaaag
     1861 acagcagcac cagcgtcacg agggtcaaag cagtgcgcat cttccggcgg cgcatgttcg
     1921 cgatccccag cagcatcgcc cggttcaaga ccgcaaaacg ccggaaatcg cccgccgacg
     1981 cgccacccgc cccgctctca gcaagagagt gcccgcgacg cagatggcga agttccgtgt
     2041 gaaaccgccc cagcacgatg gcaatgacca gaacggacag gaagagaatc acgaaggcca
     2101 gcagaatcgt caccggcgtc aggctgatgc gaaaagccgg gtgaaacagg gtgatcagga
     2161 tgaccatgac agcaagaatc cccgtgaagc cgccgatctg gcggtaaatg cctgccgcgg
     2221 cgacgagcag ccgctccagg aaatacgaga acggcactag gcagacaaga atgagcagaa
     2281 ccgcgtgaat ggcatcgtcc gtcgccgacc gggtaagttg atagacgcgt gcctgcccgg
     2341 ccagtgcacg aatcgtctcc cgcgccgctg ccgccccatc gcccgacgct aaggccgcct
     2401 cggccgcggc cagtttcttg cggctttcgg catgcagctc gcgcaaataa ggatcgatga
     2461 cccccgtgcc ctccagccgg gccatacgtt cagtattgag cagggcaaag tcacgcgccg
     2521 acgtcagccc gatcgcctcc gtctgccgcc attcaagcgg aaaaccacga ccctcaggct
     2581 cgtccgggtc ggcgttgagc accagcatgc gccgcgtcgc atccccgcga gcaaacacca
     2641 actgccagcg atcaacatcg gtcggcagga agaccgagaa ggccccttcg tcgaatgcaa
     2701 agttccaggc ccgcggctca ctgcctcgcg gcacctcgac cggcagaacc ttgtccagat
     2761 cctcgagata tcgggcatcg tgcaatccga acaagccgat ctgcgcacac tcgaacatct
     2821 cgccgcgaag gttgatccga tccgccatac gcgcatgccc cgatgccggc gtttcccatg
     2881 taccgaggcg ggtcatcgcc atgtttaccc ggcccaaggc atcaaaacgg tagacctccg
     2941 accgcagcgg agcccatcgc actttttcag gaaagatgtc gtcgaggtca aaccgcccct
     3001 ccccgtccgt catgaccaca atggcatccc gaacgccgat accccgcgta ccaaatgatt
     3061 tggcccccag atacgatttg gcgtaaagca ccgcgtcggg tatccccaaa tcgagtcggc
     3121 cctcgcccgg cgcgggcatc gccgcgatcc cagcccaacg acagaaccaa cggctcagcg
     3181 ggcttcggga caacggcatc ggccgacgca acatctcgtc aatcagcgct ccgcacatcg
     3241 caatctgcgg ggcaagtcgg tccagatccg ttcgctcctg cgtgtccatc ggcgtatcaa
     3301 cgcgcagccg catgtcctgc gtggttgcca tcatcatgcc gaggcagccg gcgctcaccg
     3361 cagcgtcgat cccgttggcc accggtgccg gcaggtcgct ctgccagtga cggcgccctt
     3421 cggcggcatc atccagatac gtccgccgaa cctcggccgg caaatcgatt cgttcggcgg
     3481 catgacgcaa ccactcccca tagccgacaa ggcgattctg aacgagctgc tcgcacaaat
     3541 ggctctgggc aaagggcgca aactgccgcc catgtgagct cagctccagg ctgatgaata
     3601 gaatcggccg cgacgcactg ccggctccca ggccgacggt ccgcaagatg tccatgtcga
     3661 cgcgccgccg ctccagacgc ccctttcgat cggcgatcat ccgatccagc cgccgctgaa
     3721 catcgggcag caaatcgcca agcagattcg cgtccatctc gtccggtctg tcttgatgga
     3781 gctgacgctg aagagccagc atccgaatgc gacgctgatc gatcgacgcc aaggccgcct
     3841 catcctcgcc gcccttccgc tgccgcctcg cctgcgcctg caactgaaga atctggtact
     3901 tgacctgcca gatcacgtgc tcgttgcgaa aacgccggtt cgaccagccc gccaccgccc
     3961 caagcacgtc tgaccccgcc agcttctgcc ggtattcgcg caaccagcca agctcctgct
     4021 cctccgcagc cagcgagccc cccaacgcag cctgtgcgtc ggtgagcgac ttcatcagtt
     4081 gacgcgaggc ggccagttcg aacgtgtccc cacaggtgaa aacagccaac acgccgtagg
     4141 ccggcgggcg agcagccagc ccacgcacca gttgcagcag aaccgcagga ttgatggcct
     4201 gctcggcacc aaaagccaga tcgggaaccg cacatgccgc atcatatcga ctatgtaaga
     4261 cggcaaccgc ctggccatcg ccccgctcgg cggccggaat gtacccaatc aggttatcca
     4321 ccgtccgtcg ttgccacctc accagcgacc gaacggtcag cttgaggctc ccgtcccgac
     4381 gcaagcgatc gctcagagcc gcgtcattca cgtagaaccg cggaaacggc acggacgcat
     4441 acatatactt gccgttggct tgcgtccatg aggtgtcttg cgggcgaaga aacaaaacag
     4501 ccagggcccc atgcatggcc gcgtattgcc agcgatcacc cgtattaaac tccatcaccg
     4561 caatacgacc ggaaagacga tccaccggca gcgcttccaa ctcgccttct ccgatgtaca
     4621 ccgcctcgct ggtgaagccc tccggcggcg tgacacaggg attcgcccca ttcggccaga
     4681 gcggatcaat cggcaccggc ggctcgtcat tgactgccag atcggcgtgg tcggtcacgg
     4741 gcaccacgag ttccaccggc cgacgccaga tgcgagagac gccggctgcc ttcatctctg
     4801 actcgatgta cgcagcagtc tgctcgcagc ccggctggcc ggcgaaacgc gagccgaacg
     4861 agcagatccg gccatggtcc tcctcgaatc gctcgcgaat gatgcttacc gcctcggcac
     4921 cgctcggccg gcggccctct tctgccgccg gccagaaggc gccgacgaca acgacaagcc
     4981 ccgcaactgg caaccggctg atcatggaac atgccatcca caattgatga tcgggtagta
     5041 tatcacgaca cacaccaacc aacgaagccg gcccgctcgg ccggccgtcg atggacattc
     5101 accgtttgcc ggagtatgat gcaactgttt tcggtgctcc accggtcgca tcgattgcgg
     5161 gcaacctcgt cagtcgtcgt aatcgaaccg atggaccggt cttcaccaag gagccggaat
     5221 atgagccacc cctcggcaat ggtcgcaatg ttcatgtttg accctgttct gcgtgggact
     5281 gagcgttgtg gaaccgtggc ctcgggggat atcgacgcgc ccatcacagt ccgtcaattg
     5341 agagactgag gcccggtacg aaaagccgcc tgccccgggc ggcgatcact ttttgaggag
     5401 acaacgtcat gaaaaagaaa atcagaaaaa tcatcctggt cgtggtcgtc gtcttggtgg
     5461 tgctggtggt cgctgtggtg ctcggaatcg atcgcatcgc caagaccggc accgaagtgg
     5521 gcgccaccta cgccctcggc gtccccacca cgctcgacag cgcggacatc ggcatccttc
     5581 aggggcgaac cgagttacag cagctacaag tgaacaatcc cgagggtttc gacagccccc
     5641 acttcctcaa gctcaaccgc ggggccctca ctgtgtcact cgggtctctg accagcgacc
     5701 tcgtcgaggt gcccgagttc acgctggaag gaatcgaggt caatctggaa aagaaaggcg
     5761 ctcagagcaa ctaccaggtg attctcgata acctcaagaa gtttgagtcc cagggggaga
     5821 gcgaacccgc aaagaaggaa accaaggagg gcaagcggtt cgttatccgt gagaccctca
     5881 tcaaggatgt gaaagtccgc gtcaaggccg cgcccctggg tgccaccctg atcgatgtca
     5941 ccgtgcccat cgacgagatc cgcctgcgcg atgtgggcag cgattcgaac aagggcgtcc
     6001 tcgtctccga actcaccggc accatcctga aggccatcat cctcgcgacc gccgagaaag
     6061 gcggcaatct gattcccaag gacgtgcttg gcgatctcga cggcgcgctc aagagcctgg
     6121 cttccctcaa ggacgtcggt gcctccctct cggtcggcgt gggcgaagcc gccaaggaga
     6181 tcggcgcagg ggtccagcag gcaactcagc agctccagga aggcaccagg aaagcggccg
     6241 aggaggcgca aaaggcgtct gaacaactcc aggaaggcgc taagaaagcc gccgatgatg
     6301 tggccggcaa gataggcgac ctcctcaaga aacccgagga caagaagccc gagtaaccgg
     6361 ccatggctgg acgaccccag gaccggcctg cagcatctcc caccctgggg caagggggga
     6421 agcattctgg aaagctctcg cagcccccag cgcaagcggc gagtccccca ggtcggcaga
     6481 gggcaggcca gggcgcaaag agaccaaggc ggagcacctt tttgcgggcc gcaacccaag
     6541 cgtaccgcga tccagaaaga tgcgcgcaag ccgcacacat atccggccgt aaggttctaa
     6601 cctcggcaag cgtatcaggg attctggggc cggcggtacg tgagacatgc cgacgagggc
     6661 ctcggcgccg ctgaggaatc tgctgaggga atcgctcgac gcaactcatc atggtgtctt
     6721 gaccgtcgct gcggcaagaa tgcgctaccc tgatttcatg ttgttcggac gcctgaatga
     6781 gccgctctga cgcaatcgcc atgtggtcag ggccgcgcct gggcaacttg ggggaatcgg
     6841 ccaaggacca ttcgctctcg catgaggttc cgaacgagcc gcctgaacgg tccgggttcc
     6901 gcgatatgtg gcccgatggc cggggacttg aatctctcgc ccaatcggct caaatacctg
     6961 ccttacacgc ttccgagcag taaggaggtc agacgtgtcg aggcgtattg tggcgatcag
     7021 tgttttgttg gctttgacag cgagtgcatg caacatgagt agcgtgaaga cgattgatct
     7081 gcgaaccgaa tacctcagcg atccgatggc catcgacgac ccgcagccgc gtttgagctg
     7141 ggtgctggat gccgacgcac gtggcgtccg gcagaccgct tatcatgtgc tggtcgcctc
     7201 aagcgaggcg ttgctcgccg aggacaaggg ggatctctgg gacagcggca gagtagagtc
     7261 ggaccagatg gcccagattg cctatgccgg caagccgctg gccgccgagc aggacgtttt
     7321 ctggaaggtc cgagtgtgga ctcaggacaa ccaaccaacc gcgtggagcc gcatcgcccg
     7381 ctggcgaatg ggcctgctga caccggcgga ctggaaggcc aagtggatca gcctgcccgg
     7441 tccggcaccg gtcgccaagg gcctgcccgc ccgtccgtcg ccgatgttcc gcaaggaatt
     7501 ccggatcggc aagccggtcc ggcgcgccgt ggtaacggcg acctcgttgg gtatgtacga
     7561 gcttcgcatc aacggccggc gggtagggga cagcctgctc acgcccgaat ggaccgacta
     7621 ccgcaaacgc gtcgagtacc agacctacga cgtaaccgat cttgtgaaga cgggagccaa
     7681 cgcggtcggg gccacgctcg gcgacggctg gtacgccggt cgactcggca tatcgcatat
     7741 cgtcaaggaa ggaccgcttc ggggtcacta cggcacgaag ctggctttgc tgatgcagat
     7801 gcgcgtttac tatgccgacg gttcctcgga tttgatcatc accgacggca cgtggaaggt
     7861 cactgatgac ggcccgatct gcaaggcgtg catcctcgac ggcgaggtct acgactcgcg
     7921 caaggaactc aaggggtggg acgcgcccgg ttacgacgat tcggcgtgga agccggcgga
     7981 attgacgggt gaggtcaaag ccaggctcgt tgcccagccg aacgaaccga tcagagtgac
     8041 cgaagagctc cgaccggttg ccatgaccga acctcagccg ggcgtttacg tcgtcgattt
     8101 cggacagatt ctgtcaggct ggtgccgcat cacggtcggc gccccggccg gaacgaccgt
     8161 gacactgcgt cacgccgaag tccttgactc ccacggcatg atctaccgcg ataacctgcg
     8221 gatgaagcct ctgggtggcg agttgggcgc ccgccaggag gaccagttca ttctcgacgg
     8281 cagcgggtcg cagaccttcg agccgcattt cacctaccac ggcttccgct acgtcgaggt
     8341 gacgggcctg cccgcacggc cggcgaagga cttcatcctg gccagggcgt tccactccgc
     8401 accgccgacc accggcacct ttgagtgctc cagcccattg ctgaacaagc tgatgaagaa
     8461 catcgtctgg acgcatcgcg acaacatgca cagcgttccg accgactgcc cgcagcgcga
     8521 cgagcggatg ggttggatgg gcgacatgct cgtttttgcc cagcccgcct gcttcaacat
     8581 ggacatggcc gcgttcttca ccaagtgggt cgtcgacatc cgcgacgatc aggccgacga
     8641 cggccgtttc cccgatttcg ccccgcatcc gtatgacccc aacgcgcgat tttccggcgt
     8701 gcccgcctgg ggcgacgccg gcgtgatcgt gccgtggaga atgtacgtca actacggtga
     8761 cacacgggtg ctggcagagc agttcgacgc ctgtcatcgc tgggtggact ggatcctcag
     8821 caagaacccc gatctgcttt ggacgcacga tcgcaacaat gactacggcg actggctgaa
     8881 cggcgacacg cttcagctcg agggttttcc caagggcgag gccgaaattc ccaaggaagt
     8941 cttcgcgacg gccttcttcc agcacagcac cgaactgacc gcgaaaatgg cccgcgtgct
     9001 tggccgccaa gcggaggccg aaaaatacgg caagctggct gccgacatac gccaggcgtt
     9061 catcaaggcc tatgtgagcg acgacggccg ggtcaagggc aatacccagt cggcctacgc
     9121 gatcgcgctc gatttcgatc ttgtccccga ggacaaacgt gaagccgccg cccgacacat
     9181 ggtcgagcgc atcgccgcgt acaagaacca catctcgaca ggcttccata ccacggtcat
     9241 gctgatgaac cagctcagcg ctgccggccg aagcgacgtg gcctatatgc tgatcaacaa
     9301 ccggacgatc ccttcgtggg gttacaccat cgatcaggga gctacgacca tctgggaacg
     9361 ctgggacgga tacgtgaaag gccgcgggtt ccaggatccg tcgatgaact ctttctgcca
     9421 ttacgcgatc gggtcggtcg gcgaatggat gtaccgctcg attctaggca ttaatccgga
     9481 tgagcagtgc ccgggttatc gccacttcat cctcaaaccg cagattggcg gcggcctgac
     9541 atgggcaaag ggcggctacg attcgatcag cggccgcatc gtcagccaat ggaaggtcaa
     9601 tggcgacgcg ttctcatggg agatcgtcgt tcccgccaat acgacagcca cggtgtacgt
     9661 gccgactgcc gacccatcga cggtgaccga atccggcaag ccggctttgc tggccgacgg
     9721 cgtcagcccg gacggtaagg cggccggagc ggcggtgtac aaggttgtct ccgggactta
     9781 ccgcttcgcc tcgaaactga aatgaggcct gcccattcgc aatcccaagt ggtgatcagg
     9841 atgtgttcaa gggttccagg ccaaaccgcc gatgcatccc agagggtgcg cccgtataat
     9901 ggccctgtta ttccagggtc cgcggcccgc gcagacgcat tcctgactgc ggtttgagtg
     9961 gagttcttcc atgagaaatt tgatgctttc ccgcttgcgg tttcggtggt tgatctacgg
    10021 cgcccttgcc gcgctactgg ccggttcgct ggcctgtgag ggcaatcttc agcttcttgc
    10081 gatgctcttc aatacttcca gcctcggagg caccaccccg gggcgtcgag ccaatctgct
    10141 ggtgaagttc gtcaaccaga cggcttatcg gccggttttc actttcggca cctatgatcc
    10201 tctgaacgac agacccgcca acgaccccta ctttcccccg aagttcaggc agttcgtgat
    10261 cgacacgagc ggtggcggaa cccgccttga gccttacagt gagagcggtg tgatcacctt
    10321 tagcggaagc ggcttgggcg accccggcgg atgcggcagg gccatcagtc tggggaccga
    10381 agacttcata cggcgcatcg aagatgacga aaccctgctg gctaatgccc gtcccgaagc
    10441 gctgcggccg ttgtgcgtga acgacgagcc caaggccggt atcgcgtttt tccgtgaggc
    10501 cagcgcgggg ccaagtgaag acccttgtga gaccaagggt gagatcgccg ccaccgccga
    10561 gcccatgagg atcttgcagg gcgatccctt gaggaatatt accaatgacg tccgttaccc
    10621 ctgtgacggc gagagcattg tggtgatcac gtttgtcgaa gaatccgccg gcgtgatccg
    10681 catcgacgtc agtgtcgaac cacccgctga ggacgagtag cagcaagacg attgcccgca
    10741 tggccgcgtt caggaggcct gtgagtcgtt cggcgagggc gctgagggtg ccagcggcat
    10801 gccgatgatc ctcgcttcaa agaccatttt accgtctacg aatccttgag catcgcagat
    10861 cgtccggcgc gggcgcacct caacgatctt ctcgacgatg tacatcgtcg ccgggggggt
    10921 gaccgccatt ctgaacttga catcctcgag acccccgaac gccaggaact tcgtggaagg
    10981 ttggacttcc tgcgaaagat aagaggccat ctgagcggcc gtctcaagca tcaggatccc
    11041 gggcataatg ggttgtccgg gaatgtgacc tcgcacccag aactcgtcgc tcctgacacg
    11101 gcgcagtccc accgccagac cggcttcgcg gtcgattcgc agaatggcgt cgagctgcat
    11161 gaattcgaag cgatgcggaa ggcgctcgta gattgccttc cggtcggcaa cgacctcgtt
    11221 cgggttgatg ctgctcaaat cggtaaaggg cgtggcggcc atacccaacc tcacaagacg
    11281 cacaaacaaa aaggagacct acccgcccct tgccgctgct gagcggagcg tcctgccgtg
    11341 accaggacgt tgccttccag aatgcccgtc ggacagctct cgggtctgat cgcaggatgt
    11401 ccacaagggc ctgggtttag acctcgcgtc tcgcgggagg cggacaagga ccgtttatcc
    11461 cgcgtcgcag gcggggcact gaagcagtat tccgagccgc aatcacgggg ctgctagtcg
    11521 cccgcggacc gtgcctccag gaccttcttc cggagttcct gggcggccga cttgacatcc
    11581 tgcatgatct ttcggacgcg ggttccggcg gccttgttgc ccccttgggc cttggctacg
    11641 tcatcagcgg ctgactcaac caggcgtttc agcttctcgt aatcttcaag cacagctttt
    11701 ctccggtaac aacctgtccg cccgtcccgg tctaccccgt tcaatatcat cggccagagg
    11761 gggacggcga tccgaacctc cgaccgcaga gtcgggagtc tagcagacag gcatcctcat
    11821 gcaaggtcag aatggggcct gaatccaaaa aaaatggctt ccgggccaga aaaacgaggt
    11881 tcaccgtggg ctggatggcc cgcgggatgt cagcattatc ctgtggcgat aatccgtcac
    11941 gcattctgcc gacagatctg ccgagtctgt tcgacatcct tggcgatttg ttggcaaagg
    12001 tcatccaccg tttcgtaccg ggcctgagac cggagccaag cgaggaactc gatccgcagg
    12061 ctctggccgt atgtgttctc cgcgaaatcc aggagatggg cttcgacgac cagtcggtgt
    12121 tcgtcaaagg tcggggtccg cccgatgctg atagctgctt ggtggcggcg gccggccagt
    12181 tcggcgaagc ccgcatagac gccctccgcc ggtaccagtt gaccgcctgg atcgatgttg
    12241 gccgtcggaa atcccatgcc cgctcctcgg ccaacgccgt gaatgaccgt ccccagtagg
    12301 gcatagggcc gtccgagaca aatcgcagcc tgatcgactg ggccggcggt cagcagttgt
    12361 cgaacaagcg aactcgaaat gacggcgtcc ggatggccgc ccagggctat ccggacgggc
    12421 tcgacgatct ggacctcaaa cccgtgccgc gaggcggcgg cttgaagtgt ctcgacgtct
    12481 ccctgacggt gacgaccaaa gccgaaagac cgaccttcca cgacggccga gggtcgaaaa
    12541 cgccggacga tcacgtcttc gatgaagtcg gcagcgctca gttggagaaa cgcagcgctg
    12601 ctgtcggcta cgacgatcac gtccgcgccg gcccgctcga gctggcgcag tttttccggc
    12661 aggggagtca gaacggcggg cattctttcg ggcgtcagaa cggccagcgg atgcggatcg
    12721 aaagtcatgg ccaccagttc agtcccggcg gcccgcgctt tctggcgacc ggcttgaagg
    12781 atggcctgat gtccgcgatg cacgccgtcg aagttgccga tgcacaagac gacgccgctc
    12841 atcggctcga aatcaagacc gcgtatcacc cgcatcgaca ctcagtctcc aatcagcctc
    12901 tcagagcgga ttgcggtgat tgccgccctc atcagcttgg cggttaccga tttgccggca
    12961 gctcgggcgg tgccggcagc ggcttgcacg cctgcgtccc ggtcgccgaa gccctgcgga
    13021 atcacaaccg ggacggtaac aacgccttca tcggtcgccc aatcgtcagc cgcccagcag
    13081 gccgctcttc tgccattgct tgaccagcac cttgagttcc tgtgccgcct cgccaaggcg
    13141 tcgcacggtc aacatcaact cttcatacaa cttcggatcg cgcagcagca tggggaccgc
    13201 tccatcgccc tcgttgagag tctgccccgt ggccaccagt tgatccaata gccgcgaaag
    13261 ctgctcgatg ttggtgacca gtttctttcc gagggtgtcg atgtgctcgc gcgtgagcgt
    13321 tacggtcgag ttgacgttcg tcagcacgtc gctggcctgc gtcgccgcga cttgcagcct
    13381 gtcggaaaag tccttgaacc cggtcatcgc gagcttggcg tcctcgctgg cctggcggag
    13441 gttggccaaa acggcccttg cattagcctg gctttcaggg tcgccaaaga cgtcatccag
    13501 atgcttcagc aacttgtgca gtctctcgac cgtcgtgaag agattcgccg tgatctccgc
    13561 cggcttgtta ggactctcga tcaggctgat gggccggtat tccagcaact cggtgacggc
    13621 gttggcggcc ggcgtcaggg cctcggccag cgttccgatc tgggccgtcg tcttttcgag
    13681 cgtgctgatg aactgcgggc tcaggatctt ctcaagcgga ttgacctgct cgcccaggat
    13741 ctcgccgctt ccgtctttgg gtagcgacgc caacctttgc ggcggaatga cgatcggaat
    13801 ccggccggtg ggcgggagga tgttgatgac cggctggccc atcagaggag attcgacaaa
    13861 cgccttccat ccgaccggaa tctcgtactt gcctcgaacg tcgattttgg caacgacgcc
    13921 ctccgtcggg ttctccggat tcttcagctc gacgttcagg acgctgccga tctggacgcc
    13981 cgccagaaac acctgtgtcc cctcccggac gccggatacg gtgtcgaacc gggccgtgat
    14041 cgtataacct cgcgtcagaa actggaaccg cgactcgccg aacatgacca acacgatgcc
    14101 gaaggcgatc agccctccga gcacaaacaa tcccacaaga gcgttgcgtc gttgatcggt
    14161 catttcgaag cctccccgga aggcacgggc tcaatgacga ggtccgcatc gccggtgacg
    14221 aactgacgaa cccgttggtc cgtcgagttc cggaagtcat cggccgtgcc gtcggcgatg
    14281 aaccggcctt gtgataacat caggattcgg tcggcaacct tgaaagcgct ggtcatgtca
    14341 tgggtcacca ccacgctcgt gacgccgagt tcctgttgca gtttgatgat gaggtcgttg
    14401 atcgtgtcgg cccggatcgg atccagcccc gtcgtcggtt cgtcgtagag gatgatctcg
    14461 gggtccaggg cgatcgcgcg agccagcgca acgcgttttc gctggccgcc ggacaactcg
    14521 gccggccgct tcggctggat gccgtccagc ccgaccagag ccagcttttt cgccacaacg
    14581 gcgttgattt ctctttccga caggtgggtg tgctcccgca gcgggaaggc cacgttttcg
    14641 cccgcactca tcgagtcgaa cagggcgccc atctggaaca gaaagccaaa acgctttctg
    14701 accgggccaa gctgccattc cttgagtttg tcgatccgct gtcccccgac gtagacctca
    14761 ccttcatcag ggtgaatgag cccgagcatg tgtttcagca gcacgctctt gcccgtgccc
    14821 gactctccga tgacgaccgt ggttttgccg cgttccaggg ccaggtccac gccgttgaga
    14881 acggtcaacc gcccgaatcg cttgtggacg ttgcggaact cgacgatcgg ctcatctcgg
    14941 gtcttcatgg tgtttcccct caaggggccg gcctccgtca gaataccgac ttgaaccccc
    15001 acagcgactc gtacagaccc ttgagaccga aggccaggaa gaaatcgagg atgagaatca
    15061 cgacgaaccc gagcacaaac gattcggtgc aggccttgcc gacgccctcg gcgccgggct
    15121 tgcagttgaa acccttgtag caggagatca ggccgatgca agccccgaag aacacgctct
    15181 tgagcaagcc cgaggcgaca tcccatttct ccagagcggc ggcggtgtag tcccagtaga
    15241 cgctgttggg aatgtcaaag agtgggacgc agatgaacca tccgccgccc acgcccatca
    15301 ggtcgcagta gagggtcagc accggcgtga gcaggacgca cgccgcgaac cgcggagaca
    15361 ccaggacgcg gatggggtcg gtgcccattg atcgaagggc atccagttgc tccgtgacgt
    15421 tcatcgtgcc cagttccgcg gccagagcac cgcccacacg accggcgagc atcacgcccg
    15481 ccaacaccgg acccaactcc cgaacgaccg agaggctgac aatcactccc atccgctcgg
    15541 ccaagccaac ggacatgaac tgctcgatgg cctgcacggc cagcaccatg cccacgaaca
    15601 cgccggtgat caggatcaca gggacgctga gtgtcccaat cgcgtaacac tgcggcagga
    15661 atctctggat gtcgcggcgc cgcagcaatg tcagcggcga atagcgtatc gtctggccgg
    15721 caaacagcca gaaatcacca aaatccgcca agccgcaaat gaccgctcgt ccaagcgagg
    15781 ccagccaagc ggtttcaggc gggcatccag gtgtggcgga actgtcctga gcggacatcg
    15841 tcgtgacctc cggcacagcg tctaccaagt atcctccata tctctatcgg ggggtcccaa
    15901 ggatgtcaaa tccttgcgac aacctcagtt atgacaacgg tcgcgatgca atgccgttct
    15961 tgacagattc cctccaccgg gaacaatagc catctgagaa tgtgtacgag aatcgatgga
    16021 gccggtcggt agggcgatgt cgaggtcgcc gcggcggcca tgacccgtca tattccgcgc
    16081 caggaccggc aggtcgccgc cctgctttct ccgcgggatt tcgcggggcg ttgaccaggg
    16141 cccgccgttt tcatgcacat tctgagactc gtaccaaggc gagcggtcgg caacgggttg
    16201 gcgagtcatc gaggcgcttt ggggtgccca atgccaagcc cgttgatcgc accgcacctg
    16261 taagcaacga gtacaccggg catggccacg ccaaggacca gaggacgcgt tccaagcttc
    16321 aaggagagag gcatcggcct gttgatcctc gcgctcctgg tcctggtggt gttcaccttc
    16381 ctgttgtcga gtggcctgtt cgccggtgtt gtccaaagcg tgtccgccct gcagaggctt
    16441 aaaggactca tcgggctcag cgaagatcgt ctctttgaca ttccggcggg caccttggca
    16501 tccgccgcgc cccccgctga aattgagatt gcacgcagac tgctgtcggc tccctcgcag
    16561 gggaaagaag gaaacggtcg ttacgcggca tacgccgtca gtgcgggcga aacggtcgag
    16621 gcggtccgag aaatggaagg accggcgggg ggctccaagg tcattgtgcg ggcgcaggag
    16681 cagccggacg cgctgggcaa ggaagcggcg gggctgggaa ccggttggat ttacgaacac
    16741 cgcccgttgg ccaaggacgc cgggttcctt cttcgggtcg tcgatatcgg ccacccctcg
    16801 aacgcagaga agctggctgc tgccaggaag cccgctgacg caaagccttt aggccttggc
    16861 aggggaggct ggatggacga aaagaatggg gcctgcggct tctggtccgg tcgctattac
    16921 accgaggttt cacttgccga ggccgcagag gtcgggccaa tcccgatggg cgagctgcgg
    16981 cagatagcga ccgagctggc cgccaaccaa ctggtctatg gtgcggaagt tgagccggca
    17041 gcaccgacgc cgaaagagac ctccgcgaca ccgacgcctg ccgttgcccg cgtggcggat
    17101 ttcgcaccgc tgcccggcgg gcaggcgctg cctccctcaa agatcgaccg gttcacggac
    17161 aacctctacg agaagatcga cggcaaggag ggcatgttcc gggccttcca ttttgtcgag
    17221 cttcgtttcg gacagtacac cagcgctcgg tcacaggagc cgttcgacgt ctacatctat
    17281 gatatgggcg aacctgtaaa tgccttcggc gtctacatgg gagaaagaag tccgacggcc
    17341 agggcatggt cgctgggtcg cgacgcctac gtttccggca cgagtgccta tttctgcaag
    17401 gacaagtact atgtcaacgt gttgggcccg cccgagggcg gcgatgacaa gctcgaggtg
    17461 tccaaggcga ttgccgcggc cattgcggcg accattccgg acgccgacga gccgttctgg
    17521 gtcgagcaat acctgccggc cgagggccgc acgccgaaca gtctgaaata cgaggccacc
    17581 agcggcttgg gctacgattt catccaccag atgttccgcg ccgaatatcg ggtcgagggc
    17641 gggaattttc aaatcttcgt gatgaaaacc accgatgcgg ccgccgcccg cgaggttttc
    17701 gacaaatacg tcgaggcaac cgccaagtac gacaaggtgc tctccaggga agctgatgca
    17761 caaggacaga agatgatcgg cgagtcgctg ggcgtataca gcgtcgcctt cacgaagggt
    17821 gttttcttcg gcggcgtcac ggaatgcgag gatcaggctc tggcggtcag gcaggccgag
    17881 gcctttcggg caaggctgcc ggctgacccg gaaggtgcga tcgcgccttc atcgagaccg
    17941 atcagctctc cggccgatga cggccaaggt tgagatgtcg tggtgagtga gacaggtcga
    18001 cacgagaacc ccacagagac gaacgcatcg cgaacatgga cgcgtcgcga cctgatcgtt
    18061 cggggtggcc ggacgattgc ggttgccggt ttggcgacgg gggcggctgt ctatcttcat
    18121 gaccccaagg gtgatgcagg cctccaacct cgcgcgccca tcaggctcaa ggattacttt
    18181 gccaagataa aacccgacta tccggcctcg gctccgcgat tgtcggcagc ctatggcgag
    18241 ccggaacgaa tcgccgatct gacgcatatt cgtgccttgg tcgaggctgc cgttgccggc
    18301 ttggatcgcg acttgggcat atcgaggttc atcagccggg gtgacgtagt tctactcaag
    18361 cccaatgtcg gctttgatcg cgacccgcgt ctgggcgcta ccaccaaccc cctggtcgtt
    18421 caggccgtaa tccagctttg cagacgagcc agggcgggca ggattatcgt ggccgacaac
    18481 ccgatcgaga gccccggcgc ctgctttgcc aagagcggaa tcgccgccgc ggccaaggcc
    18541 gaaggggcga cagtgatgat ctactcgtct gctcatgaaa gggcggtcga gatccggccc
    18601 ggccggcccg aaccggcccg caatgaggcg ctcggtacct ggccgatctt ttggacgccg
    18661 ctcaaggagg ccgacaaggt catcggcctg ccggtgatca aggaccacaa cctcagctcc
    18721 gcctcgatga ccatgaagaa ctggtacgga ctgctcagcg ggcgtcgcaa tcagttccac
    18781 caggcgattc acgacatcat cagcgacctc ggttatatga tgaagcccac cctcgtcatc
    18841 gccgacggaa cgcgggtcat gatccgcaac ggccccacgg gcgggcgcct cgacgacgtc
    18901 caggtcggcg gcatcaccgg ccggccgacg attgtcgccg ccgtcgacca ggtggcctgt
    18961 gatgcgtggt gctatcagaa cctgctcggg cgcgacccgc gggatgccaa ggccccgttg
    19021 cagtacatcg aactggctta tgcgaagttc ggtcaggatc ccggtcgcat cgtcgcaaga
    19081 cactggcagg attacgaacg acagggcctg atcgcggaga cgaagctgtg aattggatat
    19141 ttggccgtat ccgtaggatc ggctacgtgc tgatcgctga tcgctgagcg ccgaaagctg
    19201 acagctatga agattaccac cgttcgcatc atctcgcaga tcgtcttcat ggtgttgttc
    19261 ctgacgttcg tgttcgtcac gacgttcacc tgtctggatg atgccccggc cctgaagttc
    19321 tgggtgagca agtacctcga gatcgacccg ctgatcgcca tcgccaccgg cctggccacc
    19381 cacgcgcttt atcagggatt gatctggtcg ctggcgatcc tgattccgac gctcctgctc
    19441 ggccggttct tctgcaactg gatctgcccg ttcggcatcc ttcaccaatt cgtcggttgg
    19501 atcttcaacc gccggagcac ccccgaccga atcgcctcca accgctatcg cccgatgtct
    19561 cagacgaagt attacgtcct cgcggcgatg ctggccgcgg cggcattcgg gagcctccag
    19621 atcggcctgc ttgatcccat ctgcctgttg caccggtcgt tgacggtttc cgtcctgccg
    19681 gccctgaaca tgccccccgt gttcgtcgag atattcggca acccgaagat gcatcaactg
    19741 ggctgggtcg tcggcgggat cgtgctgttt ctcgtgggga tgaacctggt cgtcccgcgg
    19801 ttcttctgtc ggacgttgtg cccgttggga gccctgctgg gcctggtgag ccgcttctcc
    19861 ctctggcgga ttgatcgcga tccgaacaag tgcacggaat gcggtctctg cgaccggaat
    19921 tgtgaagggg cgtgtgatcc gggcggcaag ctgcgcaaga gcgaatgctt cgtgtgcttc
    19981 aactgcatcg actcctgtcc gcatggagcc ttgcgttttg ctttcttgcc cgcccgcctc
    20041 ggcgaggtgg cttggccgga tgcctcccgg cgccgggcga tcctggctgc tgttggcggg
    20101 gtcatcttct atccattcac caggctggcc ggaaaaacca cgcgagattt ctcctccaag
    20161 gcgatccgtc cacctggcag cgtcgaggaa caggagtttc tcgcgcgctg cctcaagtgc
    20221 ggccagtgca tccgagtctg tccgaccaac gtgctccagc ccgcaatgct ggagacgggc
    20281 ctggaaggaa tgtggacgcc cgtgatgaac ttccggatgg gcttctgcca actgaactgc
    20341 accgcctgtg gtcacgtctg tccgaccgga gcgatccagc gactcacggt ggaacggaaa
    20401 caaggtctcg gcgaatttgc ctcggccgga ccgatccggc tcggcacggc tcattacgat
    20461 ctgggccggt gcctgccgtg gtccaagaac atcccttgcg tggtgtgcga ggaagtgtgt
    20521 cccacttcac ccaaggcgat ccataccgag caacgactca tgcttgtccg ggatggaaag
    20581 aagatggtcg tctcggccac gccggtgacc gtgaccatct cggaataccc gccggaaggc
    20641 cagagcgtcg ggcctcggtg cgttttgacg cccaacgctt accggggtga tccgacggca
    20701 cgctactacg tgcaggtatt ccatcgggac ggcacgagcg aaactcaccg cattattgcc
    20761 aacgacgtcg ataccctgat gatcggcgag ctggacccgg ccggggggca gttggtcgcc
    20821 ggctcgcggt tggcccggat tcctgagcgg ggcgatgtcg ccgccatcca tatcgagttg
    20881 aaggtcccga agatcgatgc cgagctctgc gtcggctgcg gcttgtgcga gcaccattgc
    20941 ccggtcgtgg gcgatcgccg gccggtctac gtgaccgccg agggcgagac gcgctcggaa
    21001 agccaggccg acgatgcacg caaccgctcc ttccgattgg tcaagtagcg cgtcgtgccg
    21061 cgattacccg gcaagcggtt catggcttgg cacgatgctc gacggctgtt ttcgggccca
    21121 gagccagcga gcacgccggc tgcccggacc gccccgggcc ccctccgccc gaccaggtgc
    21181 ttcgtcaggc cttaaagcaa ctctttagcc gacggccaga caggatgccg cacaccgaaa
    21241 accggccacc gcgcggattt actttgaatt cccgaccagg aaaagcgttg atccgacacc
    21301 aatcctgccg attggataga aagtcgctgc tttgagaggg gccccgactt gctgaatggg
    21361 gcgaaaacgc cgattctacc ggaccgtggc atttggcgtg cgcgtaggat gtgaagggcc
    21421 ccgtccatcg ccggtctgga accgggtctc aggggaccgt gcaacgcggc agcccgggaa
    21481 tcggcggatt ttgttagagc gggagtcaat gcgtacatta gcacacgtca cacatgaggc
    21541 ggtcgagaaa gtcgggggta tcggagcagt gctgcagggg ctgctgacct gcaacgccta
    21601 ccgcggccgc gaccagcgaa ccatcctgat tggcccgacc ttcgtcaccg aaggcagcgc
    21661 cgacggccgg ctgggggctt ccggcgaagt cttgtacagc agtgttgacg gcgtcaccca
    21721 acatcccgtc ggccggctcc tcgaccaggt tcgacgcgac ttccacgtcg aaatcattta
    21781 cggatatcgc cgcttccatg accctcatac cggcgttcgc gtggccgccg aagtcgtgct
    21841 gatcgacgtc tcacgcatga acccgcacct ggtgaacagc ttcaaggggc gcctgtggga
    21901 gacctacggc atcgactctt cccgctacga atcgagctgg gactacgaca tgtacgtcag
    21961 gctggccgag cccgccttgg ccgcgctcaa ggcgctgggg gcggccgaca gcgacgacga
    22021 gtgcgtgatt ctcgctcatg aattcatggg gctgcccacc gctcttgccg ccaggctcga
    22081 tcccagcggc gttttccgaa cggttttcta cgcccacgaa gtggccgcca tacgcaagct
    22141 cgtggaggaa caccccggcc acgacgttac cttctacaac gtattgtccg gggcgatgaa
    22201 ccgcggaagg tatctcgacc aggtgttcgg ctcccaggat cactatcatc gtcacgcatt
    22261 ggtcaaacgc agccgtttct gcgaccggat cttctccgtc ggcgactacg tcaccaagga
    22321 gctcttcttc atcggcccgg agttcgccga cgccgccatc gacacggtct acaacggcat
    22381 ccctgccgag cggatcagcg tggccgagaa acgcgtcgca caggaacgaa tgcgggatta
    22441 cgccgagacc ctgctcggcg atcggccgga ttacatcttt acgcatgttg cccgcatggc
    22501 cgtcagcaag ggcttgtggc gagacctcgc cgtcctggag cacctggaga gggaattcag
    22561 gcggatcggc aagacggccg tcctgttcgt cttgtccact gaggtgccgc cgcgccgtcc
    22621 cgaggatgtc cgtgagatgg aacgttggtg gcactggccc ctggcccacc gcgaggtcgg
    22681 ctccgatctc tcgcatggcg aggccgtcta ttaccagggc gtgcaggtgt tcaatgcccg
    22741 cagccggatg atcaaggcga tctacgtcaa tcaattcggc tgggaacagg cggtgtgcgg
    22801 cagccgcatg ccccaggaca tgaccttcat ggacatccgc cgggcctctg atgtcgagtt
    22861 cggcatgagc atctacgaac ccttcggcat cgcccagttc gagcctctga ccttcggggc
    22921 gatctgcgtg ccgtcacggg tttgcgggtg cgtcggcttc ctcgacaaga tcaccggcgg
    22981 ggcgaccgtc cccaacgttt tcgtggccga ctactgcgat ctcggaccgg gccgctggga
    23041 cgagaagaag ttgctggctc tcgatcgcga ccagcgcacc cagcacgaat acaaggtcgc
    23101 tgagcggatc gcccgctata tcatcgacac cctgccccaa accgacgccc aggccgaaga
    23161 gttccttcgc agcggttatg acctcgccgg ccgtatgagc tgggacgttg tcgccggccg
    23221 gtacatcctt cccgccatcg atgccatctg cgcgaaaaag gccggctcac aggtcgcatg
    23281 accggagcgc agcagggtcc gcgctcgcgg acgtccatcc tcgaggtcgc ccgccttgaa
    23341 gcccgaccgc ctcgcgcggt attttgaatg gccggccggc gattcacacc ggctcatcgt
    23401 tcggaggtgc gaacattttc gctcttggcg acacccttac tccccgggaa actggccatg
    23461 atgttcgata gtcgaaaaac gcaagttcga tggctgacgg cggtcgttat cgcagggctt
    23521 gttgcggcac caggatgccg gccgggacaa ccgaacggct cggattccgg aacggacgaa
    23581 ctgaccgacg aagaggtgat caccgattta cgcgaattcc atcctctcat caccgacctt
    23641 ccgccgatcc ccctgccgcc cgaactggcc cagcgcgacg atgacaccgc aaaagaggcg
    23701 ctcgatctcc tcgatcagat caactcgttc cgcaacgccg atccgctggg caccacaccc
    23761 tgggacgcga cgccgaacgg caagtccgct cgaaggctca agggcgacgg atggcagaag
    23821 caatgcgatc ttttagcctg ctattacact aaaaagcagg gcaacctgac ggtcaggtgg
    23881 acgtacctgt tcgggctccc gggagtctgg gagtcggtcc tcacctggga cggatgcgac
    23941 ggcgagcatg aatacgacga tttcacgctt gagcactggc acatgactca ggatctgacg
    24001 ctggtcatcc agacgcatta tcaatacccc gatccgcctc cgtgcggcga cggccaagcg
    24061 gccatcggcc ccggcccgca gtttcgtcgg atctacgaat tgatcgaggg cggaacgttg
    24121 tatacgcccg gccaggtgat ctacctcgac acgcgccgct atacgatcga gcaatggcac
    24181 taccattcgg tcatcggcga atacctggtc ttcagcatgc tcacgtgcac cagctatccc
    24241 gacggccggc tgatttttga gcattacacc cagagagtga gcgagccgcg gctgttgtac
    24301 ctgagttaca agggcatctg gagcagcgat caccgatatt gttggaccac ctaccgcgag
    24361 gatgagacgg tcttgtcgtg cggcggggac atgggctgcc ccgaatgcga acactgaaag
    24421 ccggcgctgc cctatgcgac gctgagatgc actggctttg cttcccttcg agcgtatgga
    24481 accgccggat ccccgatcct gcttgtagcc tgactcgacc gcatggccgt acaatgccct
    24541 tccgccgtcc cgcagtcgcc atgacggcgt catgttcgca gtgacaaacg aaggccgcaa
    24601 tccaagcagg gggcccgtta tgctcgcgat gtcggttggg agttgccgtc caagaagtca
    24661 tttcggtttg cgcgtcggcg cactggctct cggcttcgca ctgctcgccg gttgtcaggt
    24721 ccccgggtct tacaagccgc cgcccgagta tccgaccttc ccgccgttgc gccatgccat
    24781 caaggccgtc ttcggcgaga acggcaacga ccacggcggc gacctccaag gccggggaat
    24841 gcagttcgtg aaggcttttc acgaccttgg catcgacctg acgcgcatcg agttcaagtg
    24901 ggcgatcgcc gagccccgac gcggcaccta tgactggtcg gaacacgacc gcctcatcga
    24961 cttcctccac cggcacggca tcgaacccat gctgatgctc tactgcgccc cgacgtgggc
    25021 catgcgcggc acgccggaag acgagcagct cttcatcgag cgaggcgagc agaatcttca
    25081 ctccgtcgtc tggccccggc gcgagtatct gccggatttc gagcgattct gcgagaccgc
    25141 cgcccggcgc taccgcggca aagcccgcct gttcgagttc tggaacgagc ccgacggcat
    25201 ggccggtccg accgtctatc acgaccgctc cggcaaggcg gttgacgtcc gctacggcgg
    25261 cgacgccaag gaatacaccc tgtggctgcg tcacatgtac gaggccgtca agcgcggcaa
    25321 ccccgacgcc gtggtggcgg ccgggtcgct gtccgtccac gacacgcggt tcatcgaggc
    25381 gatgtacgcg gccggttgca gggaccgctg cgacgcaatc agcctgcatc cctacgccgg
    25441 cgacggcgtc aacgtcgaat ggatccgcca gtgccgcgcc gtcatgaccc ggcacggcga
    25501 ctgggccaaa cccgtctggc tgagcgagtt cggctggaac ctcggcggcg agtacgacca
    25561 gaagaacggc cgctggcccg actccgcaat tcgccaggct cagatcatca ccgcgactgc
    25621 ccccgtgatc caatctcttc cctacattac ccacgccttc tggttcacgc tcaacgactg
    25681 gagcaccggc caaaccggca tcgacccggt cggcacgcac cgcttcggga tcatggatct
    25741 gtccggccgg cgacggcccg gtttcgcggc cctccgcgac gccgtactca agacgcctct
    25801 cctgccgcgc cacgccgaga tgcctttgcc ccaggtcgtt ccgcccgccg ggccgatcga
    25861 tctggcgagc gagtctcacc tcaaactgac actgacatgc cgtgacccaa gtccgctttt
    25921 ctgccccgac cagtcggaag cacggccaca gtcacgcgcc ttggcttttg aggtgcccgg
    25981 cttgacccga ggcccgcaca gcatccctct ggcctacggc ggcagcggtc gcggacggcc
    26041 ggacggaaag gtggcgtgga ccccattcga cgcaaaggcc gatgtcatcg tgcccatcag
    26101 cccggcagcc aaccgccccc tcttgcccaa cacctacgaa gccgtcgctt cgttcggata
    26161 cgggccccag acgcgtttcc ccgtcacgtt gcccgctccg gcccgccgcg ccgacgcgcc
    26221 gcccagggtc gatgccgacc tcgccgattg gccctcgcaa tggcccatcg ccgacggccc
    26281 catgaaggcc gcgttcgcct ggagcccgac ccatctgttc ttcgcatgca ccgtccgtga
    26341 cgcccggcac gagcagcctc accagggcag ggacatctgg aaaggcgatt gcgtgcagat
    26401 cgcttttgac ccgctgcgag acgcggttcg cggcagccag tacgatatca atgacagcga
    26461 gtatgctctg gctctgaccc aggacggccc ggtgatttgg cggttcatct gcccaccgga
    26521 tagctatgtc gggccggtgg ctgacgccca cgtcgccgtg cagcgaaccg atgaagaaac
    26581 gcggtacgaa ctggccatac cgtggaccga gctgagcgtc agcgatccga ccccaggaca
    26641 cctcgccggc gttgccatcg ccgcctgcga ctggcacggc gacgaacgaa ccgtccaccg
    26701 cttcggcgac ggcatcatcg gccccaagca accctgccgc ttcgcctcca ttcgccttac
    26761 cgaatgaaac cgccgatcag ccttctcgct cgcttccctt cccgcttgtc ccccattgct
    26821 cttggtccct tcatgcgccc tcaccttgtt gccggtgttc cttctcttct acttccccgt
    26881 ggtcatccgt cgttcctgcc ttctcgccct ctcttgttct ctcccgttta cttacctccc
    26941 tgttccgcaa ccccgccgac ttatggcgga cttgtgcgct agagcagcga agcatcacct
    27001 cggccggccg gctctcgaaa aaaaaaggtc tttgggcgtg ggttggggga gcacacatgt
    27061 ccccgccgac cgatcggcct catgtccggc gtgatccggt tctcttctgc tcgccttggc
    27121 ctggtcaccg cgaccaagcc gcacaagcta tactccggtt cacccccacg tgcgtgggga
    27181 caacgcggat ctatctcctg gtcctgggtt tcaatgcacg cccccacggg gggggcgacc
    27241 gttcgtatga ctcgccgtgc tgcgatgtac attctgtttc aatccacgcc cccacggggg
    27301 gggcgaccgc cgcatatcat ttagtcggct gacgaccgac aagaggtttc aatccacgcc
    27361 cccacggggg gggcgacatc tatcacccca ccgaagcgta gggctacccg acgtttcaat
    27421 ccacgccccc acgggggggg cgacggtgtg tttgcccacg ccggctggcc tgaccacgag
    27481 tttcaatcca cgcccccacg gggggggcga cggcacgttc tcggtccgga cccacacggc
    27541 ttcagcgttt caatccacgc ccccacgggg ggggcgactc gatcctgtca gtctcgcggc
    27601 ttggcgacta cgcgtttcaa tccacgcccc cacggggggg gcgacgccca accccatgac
    27661 catcgggcgc acaaggcggt agtttcaatc cacgccccca cggggggggc gacaactcct
    27721 ggcattgggg gttcggccat gtaccaaagt ttcaatccac gcccccacgg ggggggcgac
    27781 gatcaacggt gtcactggtt gttgcgacag catggtttca atccacgccc ccacgggggg
    27841 ggcgacgatg acatcacgga gatatatcgc attgccggcc tgtttcaatc cacgccccca
    27901 cggggggggc gacgctcagt tgcatggaca atctccctcc aaactcagtt tcaatccacg
    27961 cccccacggg gggggcgacg gggtatcggg ccaggctgtc cagatttgat tgaggtttca
    28021 atccacgccc ccacgggggg ggcgacacca atgacgccaa cctgatagcc ggcccaggac
    28081 tgtttcaatc cacgccccca cggggggggc gacggggtat cgggccaggc tgtccagatt
    28141 tgattgaggt ttcaatccac gcccccacgg gggggcgacc cgggctggca gatgcgcacg
    28201 gcgtttgggc agagtttcaa tccacgcccc cacggggggg cgacggcgtc gttttgatgt
    28261 tgccgttcgc atcgtacaaa gtttcaatcc acgcccccac gggggggcga cagcatatca
    28321 ccatccgctg atccgggttg acggatgttt caatccacgc ccccacgggg gggcgacgtc
    28381 atggtccggc gaggggcgga cgtggttgac tgtgtttcaa tccacgcccc cacggggggg
    28441 cgacagtctt caaccccagg ctgttccggc gcatcagggt ttcaatccac gcccccacgg
    28501 gggggcgacg ttggtggggc tcagtaagtg tccaggccga ggcagtttca atccacgccc
    28561 ccacgggggg gcgactgcgc gaccggcaag ggctcgttgt cgccaacaag tttcaatcca
    28621 cgcccccacg ggggggcgac ccgccgttca ccctggtcaa gccagcatca aaccgtttca
    28681 atccacgccc ccacgggggg gcgaccgctg gccccgccga cgtggcccgc cgtgacccgg
    28741 tttcaatcca cgcccccacg ggggggcgac tccgcaaggc atttgcggct tttcccgcaa
    28801 gtatgtttca atccacgccc ccacgggggg gcgaccggat caggttatcg agcacctcgg
    28861 caagccgggg gtttcaatcc acgcccccac gggggggcga cttcggtcta ccgctatagg
    28921 ctgtgagagg cagccgtttc aatccacgcc cccacggggg ggcgacagga ccgcggggaa
    28981 cggctgctgc ttccataacg gtttcaatcc acgcccccac gggggggcga cgctaggtcg
    29041 gcagtcgcag aggtgtactc cgaccgtttc aatccacgcc cccacggggg ggcgaccacg
    29101 ctggccccgc ccacgtgacc cgccgtaact cggtttcaat ccacgccccc acgggggggc
    29161 gacgccagcg ccgccggccc ggatccaacc ggtggtgggt ttcaatccac gcccccacgg
    29221 gggggcgacg agacttatgg ccggctgact gccggcggag gaatgtttca atccacgccc
    29281 ccacgggggg gcgactggcg acctcgatgc tggccggcgg cgaaacatca gtttcaatcc
    29341 acgcccccac gggggggcga cgacgcgtct cgatgggcgg gattgatggt tggcggtttc
    29401 aatccacgcc cccacggggg ggcgacatgt ttgcggggtc ctcataggtt ccatcccggc
    29461 aggtttcaat ccacgccccc acgggggggc gacgcctgga tcacaaccct gattccgggt
    29521 tcatgcgcag tttcaatcca cgcccccacg ggggggcgac gccagcgccg ccggcctggc
    29581 tccaaccgtt ggtgggtttc aatccacgcc cccacggggg ggcgaccagg aaggtggcgg
    29641 cgagctggcc gaggggcgtg tttcaatcca cgcccccacg ggggggcgac aaacgctggc
    29701 cccgcccacg tgaccggcgg taactcgttt caatccacgc ccccacgggg gggcgacccg
    29761 accgcatcga cacaccaccc gagctcagat cgtttcaatc cacgccccca cgggggggcg
    29821 acgggtgccg aaccggtcat gacaccggcg ccgagtccgt ttcaatccac gcccccacgg
    29881 gggggcgaca agtccgccgc ggcgaaaacc caccccccac ctggcgtttc aatccacgcc
    29941 cccacggggg ggcgacggcc attgctgtcg atgagcttaa caagcttgcg gtttcaatcc
    30001 acgcccccac gggggggcga cgaaggggtt aggtgagaat gctccaaacc tacgagtttc
    30061 aatccacgcc cccacggggg ggcgacgcca gcgcggacga tacgctcacc ttgacccagg
    30121 gtttcaatcc acgcccccac gggggggcga cgtcggggtc gattacactg ccgtgacgca
    30181 gatggagttt caatccacgc ccccacgggg ggcgacggat cgtgcgctgg atcgacccag
    30241 ccaccggccg ttgccagcgt cgttctgaaa gtgcagagat ttcgccggtt tgaatatgta
//
LOCUS       DAOTPW010000029        30125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1605_length_30125_cov_6.349385, whole genome shotgun sequence.
ACCESSION   DAOTPW010000029 DAOTPW010000000
VERSION     DAOTPW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 30125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30125
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1605_length_30125_cov_6.349385"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(568..2547)
                     /locus_tag="P5316_04245"
     CDS             complement(568..2547)
                     /locus_tag="P5316_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84199.1"
                     /translation="MKCCRLACIVVLSLSTLAPAQVQFLGRVLHPRFPEAADKLPTNN
                     GVPEDYFAPDRPGDPAEIMPLCNVRCFASVDGPGIETMAARTWELAPSGWYRMTGSAG
                     RTTMLFTGPSVFMQPTVFTNIFTKPGETIDRKVVPNFDYAVLFMGAWDDKPATDYYQT
                     FTATGTSVTQVGFRLASDGVDGEGPGSQTLLVSVHRQGPGTPDTWQQIGPAVPVPNVD
                     CGGPKNYDWLAGWNSGEVPTEPGRVYAVHLKAEKDGNTFQAFWRPNEKKINDCYRLGP
                     RGQTGWVGHNLCMVIATDNSGLVIPYNKRVQKEYVDFAGFARKWSQSYVAQGRSLAGA
                     LLYMANSGVQPSVARQRAVVRLRQDGPDGPVVGVEKVAIGNGIHTGDASWGVYGLAYS
                     PGEVPLEPGKTYAIEFESIENYESLHGYVNIKGMVSDDKPGFNPYKKVAPDNYENGTA
                     YKNGKDDAGFDLDMQIIEYRREVTDWHKAVDPQNLLVNGDMEAGQLDEKDPGNGRIEG
                     WRTFSADPGTIHHFLADPPENKSRILRVTAGKEAKAVDGGYVQKVTGLKRLDTYRLSG
                     KVRSSWSVDVEHYSQVGYDPTGQDSDPKAPTIVWTPLPGMHSIWVEYHSEPIRPAGDS
                     ISVWLRGWARSTGGTPFKADFDEFSLRRVKTQLTP"
     gene            complement(2647..3258)
                     /gene="pncA"
                     /locus_tag="P5316_04250"
     CDS             complement(2647..3258)
                     /gene="pncA"
                     /locus_tag="P5316_04250"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HRR84200.1"
                     /translation="MKALILVDIQNDFLPGGPLAVPMGDQVVPVANRLMSQYDVVVAT
                     QDWHPANHCSFASQHDGHKPGDVIELGSVTQILWPDHCIQGTRGADFAPGLDMAGVHW
                     ICHKGTDPGIDSYSGFFDNGRLKQTGLSDYLRKRGVREVHIIGLATDYCVKFTALDAR
                     ELGFETHLILEGVRGVELHPGDCTKAIEEMRAAGVRVVTAAAA"
     gene            complement(3470..4216)
                     /locus_tag="P5316_04255"
     CDS             complement(3470..4216)
                     /locus_tag="P5316_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84201.1"
                     /translation="MGQDQAKTESCAAGDVAAQTSSNMQSDSKAAPPGDLTDVDLYCL
                     HCGYNLRGLSGDPRRCPECGLFSPLSAMTLPAEAITDQLRRMETWPTLCIAFVLLLMF
                     SLTLAAFALAVGGPTKEVCVALALPVLILVPLWLHGVSRFSSACMDKPGWWSVLIEYH
                     LYGLGLCLMLVVSSGLPLFHWLDRRSWNTSLPADAAFLTILIGGLTGVVILAPRARRR
                     CKKRMDALQREVAVKLAGEYLQRVLSRRPF"
     gene            complement(4254..4988)
                     /locus_tag="P5316_04260"
     CDS             complement(4254..4988)
                     /locus_tag="P5316_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84202.1"
                     /translation="MGQDQAKTESCAAGDVAAQTSSNMQSDSKAAPPGDLTDVDLYCL
                     HCGYNLRGLSGDPRRCPECGLFSPLSPSANMIARELGRMETWPTACVAAATLLVGSQA
                     CIVVALVNSRMPLGLCIGCWLPLLVLLPLWFTGMEQFRSDCLYKRGWKSVFVQYHIYG
                     MALSILLASAAAASIWVFHRLVRQERSFIGAIGLVALAGIGLSVFLLARRARQQCNAK
                     RHVLQREVATTRAKVQLYERLSRRRS"
     gene            complement(5295..7790)
                     /locus_tag="P5316_04265"
     CDS             complement(5295..7790)
                     /locus_tag="P5316_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRR84203.1"
                     /translation="MKKTIHLLCNAHLDPVWLWEWEEGAAATISTFRTAADLCEEFEG
                     FVFNHNEAVLYQWVEEYEPSLFERIRRLVREGRWHIMGGWYLQPDCNMPSGESFVRQI
                     LTGKRYFKEKFGVDVTTAINLDPFGHTWGLPQILAKSGYDSYLFCRPFQNDCPLPDSH
                     FVWVGCDGSEVMACRVDGWYSSQLGKAHLKIMERMNNHPDRPFSLLLWGVGNHGGGPS
                     REDLKEITNLIRNTKEFNILHSNTAAFFAEMKAQYDLLPRHKRDLNPWGVGCYTTQVR
                     IKQKHRELENQLYLVEKMATTAACQGLMEYPREELHRAMRDLLICEFHDILPGSSIEP
                     VENASLRLFDHALEELSRVKARTFFALASGQPRARGGQIPILVYNPHPFKVRTVVECE
                     FQLADANWAGTWTLLGVRRGGQAIPSQIEKEASNLNLDWRKRVAFLAELDPSQMNRFD
                     CRIEKVVQAKPVPKLKVRGGRVAFWSEDIEVVINAKTGFVDCLKVHGIDYLEKNAFEP
                     LVMADNEDPWGMLNRAYRKVAGRFKLMSAKKSAQFSGVRCDELPPVRVIEDGPVRSVV
                     EVMLSYNDSFICQRYKLPKQGTEIEIELRVHWNEKDRMLKLSIPVRGTGFEFLGQTAF
                     GINPLPMHGNEAVAQKWVAVASRRARTAITCINEGSYGADFSKNGLRLTLLRSPAYAG
                     HPFFDRPIVEQDRYTPRIDQGERIFRFWLNAGSLDERLSAVDREALVHNEKPYALSFF
                     PSGEGPRPKAGAVLSDHVVQMSCLKLAEDNDDLIVRLFEPTGHARSTTLSLPCIGFKT
                     RVNLGPFEVKTLRVNPKTRAVRQTDLIERPMGK"
     gene            7983..10196
                     /locus_tag="P5316_04270"
     CDS             7983..10196
                     /locus_tag="P5316_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRR84204.1"
                     /translation="MSRKWSVLTAGVLFVGSGAVWGVEFKDQIAGDAISGWEIRNGPP
                     DSWSVKDGVLHCHGAEKWGGWLGTVRNYTDFIIELEYKLSPGGNSGVFLRTPREGHPS
                     AVSFEIQILDNTAEKHKNLKPAQYCGSIYKIVAADKDATKPVGEWNRMRIMAEADHIV
                     VWLNGEKVVDATGKSDPEILQRSREGAIGLQNHRSEVSFRNIRIADLAEDRARRTKWW
                     REARFGMFIHWGPVSLKGTEIGWSRGGQRRGHGEGRGEIPVEVYDNLYKEFNPTRFDA
                     RQWVQIARDAGMKYMVFTTKHHDGFCEFDSKLTDYKITHSPFKRDVVAELTAACHEAG
                     MPIGFYYSPPDWHHPDYRTENHGRYVEYMHGQIRELLSNYGRIAIMWFDGLGGTSKDW
                     KSTEMFEDIFRLQPDIIINNRAGLAADHDTPEQQIGKFQNNRLWESCITICRQWAWKP
                     EDEMKSLKDCIRTLVRCAGGDGNLLFNVGPMPTGEIEPRQVERLREMGQWLSKYGESI
                     YGTRGGPFKPGIWGASTHKDNRIYVHVFEWPADGQSLALPPIGKKVLGAAVLTGGAAE
                     VKQSSEGLVITMPASERQEVDTIIAIDLDGPAAEIAPLATRLGSLAAHKKSTASNVFQ
                     KSWLHGPEKAFDDDDSTRWATDAGTKQAWLEVDLGEPTTIGRAVISEAFDRVRAFELQ
                     YRDGQEWRTFARGSTIGADKLITFAPVTADRVRLNITEATEGPTIWEFQLFAPSK"
     gene            10258..11037
                     /locus_tag="P5316_04275"
     CDS             10258..11037
                     /locus_tag="P5316_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HRR84205.1"
                     /translation="MSQKRIQIAAHKPSPWILRPRPVVSPRLRLFCFAHAGGGASLFR
                     NWPCGLSDKVEVCAVQSPGREERLDDCPYARFDALEQTTADQIKPWLDVPFALFGHEL
                     GALVAFELVRRLRRTGLPAPVRLIVAGCAAPHLPRSRPPIHRLSDDEFLDGLAGGLAA
                     VPDAVRRRPDLVARILPGLRADFTMLETYTYRPEHRLDCPITALGGSADPTMPTDALA
                     AWAEHTSTFSQQMIQGDQFFLFTGETEVLSCLANLLSPDYL"
     gene            complement(11493..11579)
                     /locus_tag="P5316_04280"
     tRNA            complement(11493..11579)
                     /locus_tag="P5316_04280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11543..11545),aa:Ser,seq:tga)
     gene            complement(12376..12591)
                     /locus_tag="P5316_04285"
     CDS             complement(12376..12591)
                     /locus_tag="P5316_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84206.1"
                     /translation="MAVSTIEGIVENGCIRLRDNVMLPDNTKVYVVVPDIETPPQARV
                     YSPHLAHPEQAADFVKHVVEVSDDAGL"
     gene            complement(13001..14344)
                     /locus_tag="P5316_04290"
     CDS             complement(13001..14344)
                     /locus_tag="P5316_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84207.1"
                     /translation="MSQDKQSRASRREFLSTSGRIAAASALGLTAIPAVHAGEGNTLK
                     VALVGCGGRGTGAAINALSTSSGPTRLVAMADVFDDRLQSSLQKLQSQFGKQIDVPKE
                     RQFIGFDAYKKAIDSLDGSGVVILATPPGFRPPHLEYAVEKGCNVFMEKAFAVDGPGV
                     RRVLKAGEEADKKNIKIAGGLMSRHSAPLEEAVRRIHDGIIGEVITCWSYREHGPVGI
                     TPKPADMTEIAYQIRHFHNFNWLGGSFILDWLIHNLDVCCWVKDAWPVSAQGQGGRYP
                     RTEADQMFDHYAVEYSFADGTRLFAQARHMADCWGFWGNVIHGTKGSAVLGEGIPDPL
                     IYKGHRRTEDQIIWRYQGEKSDQYQVEHERLFEAIRQNKPYNETKRCAYATLTGILGR
                     MAAESGQEITWEQALASDLVLAPGLESYTMDSTPPIVPDAQGRYPVAVPGKTKVL"
     gene            14558..15817
                     /locus_tag="P5316_04295"
     CDS             14558..15817
                     /locus_tag="P5316_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR84208.1"
                     /translation="MTSRERVVAALNHRQPDSVPIDFGGTAVTGMHVTCVAALRDHYG
                     LEKRLVKVHEPYQMLGWIDDDLADAIGIDVAGVIGPTTLFGFANTNWTPWRAPFGLEV
                     LVSEHFKTTMDAKGDVFIYPQGDTTAPPSGRMPDGGYFFDTIVRQEPIDEDKLDPQDN
                     LEEFGPISEADLAHFQGEVKRASATGRAVIATFGGTAFGDIALVPAPFLKYPKGIRDI
                     EEWYVSTVARRDYVHQVFDRQCEIALANLAKIHARVGDAVDAVFICGTDFGTQTSAFC
                     SRETFRDLYMPYYRRVNDWIHAHTKWKTFKHSCGAVEQFLGPFIESGFDIVNPVQCSA
                     SGMDPAHLKRTYGDELTFWGGGVDTQKTLPFGTPAQVREQVLSRCEIFSKNGGFVFNA
                     IHNVQARTPVANIVAMIEAVHEFNGRR"
     gene            complement(15938..19090)
                     /locus_tag="P5316_04300"
     CDS             complement(15938..19090)
                     /locus_tag="P5316_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRR84209.1"
                     /translation="MMPEPTAASGGNGRPGTTTFDRIMIAIVAVSLASVCLLISATHG
                     SKTGYQEWAPGSVLRNIVDLLNFNYAFPTSSGSEVKWLAHGLGVAAAVLAVTLMWFTR
                     SDVTRPITVEEPTPAPAGRRCCSTVALAQIALVLLTLWMVISSQWSPWPEASLGEGSR
                     QAIWTIWALILGRTLTRAGARGVAVAMTAILTATAALGIWYRHERSPFMRLEFPIGNP
                     LFMAACLIVGLCLAGAIVFGVLAETMDRSRERKTAPARMFAEVIRDMGGWWVAIGAVV
                     GVAVMMWALRLTESRTAQWALVAGAAVAVWLGLGRLGRWLLPVGAVGAAALFFALNWS
                     AVTSGTTGRDATIRFRLYAGKYAIQLFSTAPIVGHGQGSYMLLAQAMQAPDMEKDSAA
                     FMAPVIENAHNEYLQIAAETGLVGSVLCAAFLVLTMWGAIRAWSRVKTPLDQWLLAGA
                     LAAFTAVVIEELGDVALRKPGLPAVFYTTAGLTWALSLRLPAAREPIISSQPTPLRVA
                     GLIAGIAASLGIAWTVWRNWEGALAAYQILTRAEEQEWDASMNAARIASSGRLAVEDY
                     VEAYDGTTHVSLIAAAGSVDRCMRGAARLPQDAEPPAGLVDMIREDVEAFNRFASICE
                     ATGNTLLVVIPGYPRIAWILAHLQVYRQQVEMIERQAGLRADVRDYTAAVRTLLSVEL
                     SRDPQDMAVAMRLFELCGDESVAYRLNLLRRPLRRGPVPVRQTDRFGIDWLADMEPLI
                     GAMINEKGFSEAMEKMLADARQAMAANSADDWPDPYAPESLRLSARVSKLSGRFLEAA
                     ELSGQAAELSRQLGGRFPGAVSNARLDQSRYLMLAQRPAEAVAACDQAIKEWPIVRER
                     ELQLRPVMQTRAMYMLAADSEKEARELLGKLYPEAASDEIDQRLSDGYVDLCQSFASF
                     PIEARAASYDKWLQRALALDPGNRTTHDLAARRAAERQDDVAVVEHVKAVIRLSFEQK
                     DDSSGLQYLRLLAGVTRDENLVMALVEELSKSHPGNAMLQRLLASGPTAIFPPTTATA
                     PTDTSSAPASRPTTMTEPGT"
     gene            complement(19087..19635)
                     /locus_tag="P5316_04305"
     CDS             complement(19087..19635)
                     /locus_tag="P5316_04305"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRR84210.1"
                     /translation="MSLHTRTGDMGETSLVDGSRVAKDDPRVCAYGNVDELNSVLGWC
                     RAACEGAIAQSIVDIQSQLFHVCSELATPPTAGDKWKHLAITQDHCRRLESWINEAEA
                     AAGSVGCFVLPGGTEAACRLHMARVCCRRAERSIVTLHRVSPVRAELLAYLNRLSDLL
                     YAWTRLANRQAGRSEIPWRPNV"
     gene            complement(20104..20688)
                     /locus_tag="P5316_04310"
     CDS             complement(20104..20688)
                     /locus_tag="P5316_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRR84211.1"
                     /translation="MKAGSSLTLTIQLALSIGSVFLAGCPSASDFGTGTQVAGMGTNT
                     ESLPEGGYTPSNADVLVARMLELVNNERTSRGLQPLTLNPVLSQMADDYAKEMIACGF
                     FSHLSPTGEGPGQRAVSAGYVFLAVGENLAVGQTTPEQAMADWMKSTEGHRENILSPQ
                     WKEIGIAVRVGGEYGVYWVQEFGNPPAIARISAN"
     gene            complement(21123..22199)
                     /locus_tag="P5316_04315"
     CDS             complement(21123..22199)
                     /locus_tag="P5316_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84212.1"
                     /translation="MEEPPAFHGLTSTSCHDETAAARPVPADACSPPPDWSTIDWDVH
                     CPLCEYNLRGLNVPRCPECGYRFAWKEVLDPNRREHPYLFEHHPRRNFWSFLKTVRGA
                     MRPKRFWTSLHPVMPSQPGRLLGYWLATLLCFAGVAILILFPTNVCELAWNLIEGRQA
                     SPFSLFSGLWGWGRPPAVFVPGATRLPSVIWEFLSSLTWECNRAEKLLWMAAMLPFVW
                     PLLTLVSLKVFRWSMRRAKVNKVHVRRCVLYSFDMIPPALTMLLALLLLIGQVISGSN
                     TGWHEPFLLCLPFLSVVFCARRLYYAYVRYLRFDRPFLTVLASQMMVLLAVMIFWLPW
                     LVGVRWEIRSDWLESFVNVILQLN"
     gene            22382..22627
                     /locus_tag="P5316_04320"
     CDS             22382..22627
                     /locus_tag="P5316_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84213.1"
                     /translation="MTIEQLRAVQQATPFRPYVLQPADGDRIVVRHPELVLITPGGRT
                     IAVATAADAVKIIDLSLVTAIDIPKNHRRTRALRQTR"
     gene            23028..24002
                     /locus_tag="P5316_04325"
     CDS             23028..24002
                     /locus_tag="P5316_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84214.1"
                     /translation="MSLVIIGTGNWGSTLAGLLNPEQPLRMWCERKQFIKPTVESLKR
                     AAGADRPGVVVEEMFASPLTADDIVLVVVPSAQVGPVAQKIREKIKPPYPIIVSASKG
                     LERATFRTCSQVIASILPEATIAVMSGPNIAKEIAAGRPAKAVLGCDNVHVLLKVAKA
                     LRSDRFHLDMTRDTVDVELCAAMKGVFAIGAGVIDARKWGCNFMGLMLTFGLREIAEV
                     GRFLGISSSHVFGIAGLGDLVATCLSPDSRNYRLGQLLAGGTPLKEALSQVGMVVEGA
                     MTASAVSEMAALRLRVPLFSAISAIVEDPCEAAFQRLEQVLLEYPSNG"
     gene            23999..24709
                     /locus_tag="P5316_04330"
     CDS             23999..24709
                     /locus_tag="P5316_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HRR84215.1"
                     /translation="MKAIILAAGAGRRLGAIVPKCLLDVGGMSIIHRQLAAFSTEGIK
                     EYVVVVGYQQDRVREHLAGQPGRFVFVVNEQYASTNTVYSLYLAASYLTETCWYANAD
                     VVFDRRLIRRLAEDSSAAALAVQTHSCGEEEVKVIVREGLVVRIGKTIPPAEAAGEFL
                     GVARLSGNVAAALSRTLTELVEQQSVIADYFERGLDRLCSEFAIKAIDVTDLPCHEID
                     FPVDLEKARREIAPRLEE"
     gene            24811..25539
                     /locus_tag="P5316_04335"
     CDS             24811..25539
                     /locus_tag="P5316_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRR84216.1"
                     /translation="MLAFLGQVILVSLSGVMAPGPVTAATLTAGTRNRHSGLLIAVGH
                     GIVEFPLMIVIVAGAGMIFKQPVAQIIIGLVGGAFLLHMGGQMLAGLRKQEEIAAPYV
                     DSHPVRIGIVLTATNPYFLFWWATVGLGLSLEARKLGPTAFAIFAVVHWLCDLVWLEI
                     LSQASFRGTQILGRKSMPIILGICATALIVIGSRFVYDAGKEMIIQVFPQPTETYIFP
                     DTVPASAPATLPITQPVSTLAPVS"
     gene            complement(25593..28094)
                     /gene="priA"
                     /locus_tag="P5316_04340"
     CDS             complement(25593..28094)
                     /gene="priA"
                     /locus_tag="P5316_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRR84217.1"
                     /translation="MARSSRDAGPALWTETPEVREGPVARVAMVAAVEGQYSYAIPPE
                     LEEKVAVGKRVLVPFGRNRRPQPAFCVSVAREAWTSTLRPLADVIDDTPLLSGPLMEL
                     GKWMARYYCCPLGRALSAMVPEAIRRQRGFITVRRLMLANSSTGAVESAEAAKEGRVR
                     SEAAEEGRARSEPPETAETCQPIDNAAGLGHGGDLDAALPQASGHSHTQSEQTQEVPP
                     PGRPKRFSARQQAVLNALAAAPQGLDLDDLLARTGVGKAVIAGLLKTGHISQETRRVA
                     APAPDFDHPGPEPTFELNVDQQAAIARIASLTAEGTFRAVLLFGVSGSGKTEVYIRAI
                     RSVVAAGRQAVLLVPEIALTTQLVERLAARFERVAVIHSGLTGAKRSLTWAAIAAGEK
                     RVIIGTRSAVFAPCPNLGLIVVDEEQEASFKNQQSPRFNTRDVAIKRAQICSCPIILG
                     SATPSLEMWHNCQRLSHFEKIVLPTRVAGLPMPRVQYVDMQIEQRQRKGLHLLSQEME
                     NELAETLGAGQQAVLLLNRRGYASYLVCSRCRTTIVCPHCHVNLVFHQSTGKALCHHC
                     SARMVMPTRCSDPSCNGTLVRFGMGTQRVEEEVRTKFPQARVARADSDTMTKISAYER
                     VLRDFADGRLDVLIGTQMVAKGLDFPRVRLVGVINADTSLLQPDFRAAERTFQLVTQV
                     AGRAGRSESDGRVVVQSLVGLSPAIQFAANHDYESFARHELAIRKNRGFPPFARLTRV
                     VVSYPARSEASRHADELAERIRESLKRHGLPADVLGPQSAPLARLRGQYRFDFLIRAA
                     NAARLLQILERLRGEEILPLSDKHVLIDVDPVSLL"
     gene            28309..28947
                     /locus_tag="P5316_04345"
     CDS             28309..28947
                     /locus_tag="P5316_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84218.1"
                     /translation="MNTISRRTLLKTSAALAAASAASQTLAAGANERIRVGVIGARNR
                     GWQNAETFQKSGRFEIATLCDCDSTCLDLAMAKIEKILPVRPALEKDFRRILDDKRID
                     AVIVATPDHWHALMTVMALEAGKHVYVEKPASFNIDDGKAMVAAQKRHPKLVVAMGTQ
                     QRSGQHFKDAKAFIDDGGLGTIALARAWMAGNRVVVQKVPDGDPPKELDYDM"
     gene            28963..29580
                     /locus_tag="P5316_04350"
     CDS             28963..29580
                     /locus_tag="P5316_04350"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84219.1"
                     /translation="MKPYNKEKVHYNWHFMRDYGTNDAGNWGGHYLDIVRWFADLDLP
                     RGVSGFGGKYVVHDEKEWFDTQTALFHYPHLTVIWEMSHWNAVGPFGMGTGAEIRGDK
                     GTVFIDRGGWTFLPEKGEPVKHPSSDLEGPHVQNFADCITAGARPAADIIEGHKTAVL
                     CHLANIATLLNRTVWFDPKSQTIEGDAEAAALQGREYRKEWPMPV"
     gene            29651..>30125
                     /locus_tag="P5316_04355"
     CDS             29651..>30125
                     /locus_tag="P5316_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84220.1"
                     /translation="MMSSNLSRRAFLKTSALGASVVALGAPGVARGFPANEKVRLGWV
                     GIGGRWGNLAELMLRHVPDAANVAVCDLMPDRVERGKKVFERDKPNGYTDLRMMIEKE
                     KLDGILVATEPCNHSVVVVRVLEAGIHCFAEKPMDTTVEKVDAITKAARKAKVQTQ"
BASE COUNT         5816 a         9487 c         9264 g         5558 t
ORIGIN      
        1 ggtggcggca ggtcggcgcc ctgcttcgtc ggggcgactt ggcggggctg tgacctgccc
       61 tacctgcttc aggattcgtt tggaggcgca tgcccacgca aggccgtggg catggcaccc
      121 ggcggacctg ccctacctgc tgaatgcgga aaggattccg caggggatgc ggcccggaaa
      181 ggattccggg ccgagcggtg catacaccta ttatagttcc atatagcaca atatgtcaag
      241 agtcaagcgt tgaccccgat acccccatag cacaatatgt caagagtcaa gagttgaccc
      301 cgataccccc ccgatacccc ccttgacccc gatacccccc aaccatgccg aagtcactcc
      361 caggaccgac gcggacgacg agcagcagaa gatactcgat ctgctcggcg tgcgtctctg
      421 agaccccgat ccccgaaccc tgtagccagg caagtgaaat ccaagaccgc actcaacgcc
      481 agggccacca aggacttgca tcaagcaaca gggcgtaagt tgcggcaaaa cgcgctcagg
      541 gcgatggccg acgaagacat gcgtccatca gggggtaagt tgcgtcttta cccgacgcag
      601 cgagaactca tcaaaatcgg ctttgaacgg cgttccgccg gtcgacctgg cccatccgcg
      661 cagccagacg ctgatcgaat cgccggccgg gcggatcggc tcgctgtggt attcgaccca
      721 aatactgtgc atccccggca gcggcgtcca gacgatggtc ggggccttgg ggtcgctgtc
      781 ctggccggtc gggtcgtaac cgacctgaga gtagtgctcg acgtccaccg accaggaact
      841 gcgaaccttg cccgacagtc ggtaggtatc cagtcgcttc agcccggtca ccttctgcac
      901 gtatcctccg tcgacggcct tggcctcctt gccggcggtc acccgcagaa ttcggctctt
      961 gttttcgggc gggtcagcga ggaaatgatg aattgtcccg gggtcggcgg agaacgtccg
     1021 ccatccttca atcctgccgt tgccgggatc tttttcgtcc agttggccgg cttccatgtc
     1081 gccgttgacc aacaggttct gcggatcgac ggctttatgc caatccgtga cttcgcgccg
     1141 gtattcgatg atctgcatgt ccagatcgaa gccggcgtcg tccttaccgt tcttgtaggc
     1201 ggtgccgttc tcgtagttgt cgggagcaac cttcttgtac gggttgaaac ccggcttgtc
     1261 gtcgctgacc atgcccttga tattcacata accgtgaagc gactcgtaat tctcgattga
     1321 ctcgaactcg atggcatacg tcttgcccgg ctcaagaggg acttcgccag gcgaatacgc
     1381 caggccatag acgccccacg acgcgtcgcc ggtgtggata ccgtttccga tggcaacctt
     1441 ctcgacgccg acgacggggc catccggccc gtcttggcgc aggcggacca ccgctcgctg
     1501 acgggcaacg ctcggctgaa cgccgctgtt ggccatgtac agcagcgccc cggccaggct
     1561 acggccctgg gcgacatagc tctggctcca ttttctggca aaacccgcaa aatcgacata
     1621 ctccttctga acccgcttgt tgtaggggat caccagtccg ctgttgtcgg ttgcgatcac
     1681 catgcacagg ttgtgcccga cccagccggt ctgtcccctg gggccaagcc gataacaatc
     1741 gttgatcttt ttttcgttgg gtcgccagaa agcctgaaag gtattgccat ccttttcggc
     1801 cttcaggtga accgcgtaga cccggcccgg ctcggtcggc acctccccgg agttccaccc
     1861 tgcgagccaa tcgtagtttt tcgggcctcc gcagtcgacg ttgggcacgg ggacggccgg
     1921 tccgatttgt tgccaggtgt cgggggtgcc gggcccctgc cgatggacgg aaaccagcag
     1981 cgtctgcgat ccggggccct cgccgtcgac accgtcgctc gccaggcgaa acccgacctg
     2041 cgtaacactc gtacccgtgg cggtgaacgt ctggtagtaa tcggtggcgg gcttgtcgtc
     2101 ccaggctccc atgaagagca ccgcgtaatc aaagttcggc acgaccttac gatcaatggt
     2161 ttcgccgggt ttggtaaaga tattagtgaa cacggtcggc tgcatgaaaa ccgacggccc
     2221 ggtgaacagc atcgtagtcc gtccggcgct gccggtcatg cgataccagc cgctgggggc
     2281 cagctcccac gtgcgggcgg ccatcgtctc aatgcccggc ccgtcaacgc tggcaaaaca
     2341 gcggacgtta cacaggggca tgatttccgc ggggtcgccc ggccggtccg gcgcgaagta
     2401 gtcctcggga acgccattat tggtcggcag cttgtcggcc gcttcgggga agcgcgggtg
     2461 caggaccctg ccgaggaact gaacttgcgc cggcgcaagg gtcgacagcg acaagacaac
     2521 gatgcacgcc aagcgacagc acttcatctt cgggactcct caattggcag gtcaacagca
     2581 gtctgctctc cacgggccat ctagatattg gacaatgagg cgggtgtcaa ctgtcggagg
     2641 cggcgtctac gccgcagcgg ccgtcaccac tcgtaccccg gctgctcgca tttcctcgat
     2701 ggccttcgtg cagtcgcccg gatggagttc cacaccgcgg acgccttcca ggatcaggtg
     2761 ggtttcgaag ccgagttcgc gggcgtcgag ggcggtgaat ttcacgcagt aatccgtcgc
     2821 caggccgatg atgtgcactt cccgcacgcc tcgcttgcga aggtaatcgc tcaacccggt
     2881 ctgcttgaga cggccgttgt cgaagaaacc gctgtagctg tcgatgccag gatcggtgcc
     2941 cttgtggcag atccaatgga cgccggccat atcgaggccc ggggcgaagt cggctcctct
     3001 cgtgccctga atgcagtggt ccggccagag gatctgagtc acgctgccca gctcgatgac
     3061 atcgccgggc ttgtgaccgt cgtgctgact ggcgaacgaa cagtgattcg ccggatgcca
     3121 gtcctgcgtg gcaacgacca cgtcgtattg cgacatgagc cgattggcaa ccggaaccac
     3181 ctggtcgccc atcgggaccg cgagcggacc acccggcagg aagtcattct gaatatcgac
     3241 caggatcagc gctttcatga cgctccaact cgcggaccag gtcgcaggaa agagattcgc
     3301 tccgcgtttc ccgccgattt ggcccctgag tggcgggtcg tatcattgca gccccgtgtc
     3361 cattgctcaa tgcgggcggc gctgtgacgc tcggcgttcg gcatcatcaa acggcggccg
     3421 gccgacaatt cgatcatggc ttggccggcg gcagggacac cgtcgcccct cagaatggcc
     3481 tcctggataa tacgcgctgc agatactcgc cggccagttt gacggcaacc tcccgctgca
     3541 gggcatccat tcttttcttg cagcgacgcc gggcacgagg tgccagaatc accacacccg
     3601 tcagacctcc tatgagtatc gtcaggaaag ccgcatccgc cggcaaggaa gtattccagc
     3661 ttcgcctgtc gagccaatgg aacaaaggca gcccggacga cacgaccagc atcaggcaca
     3721 ggcccagccc atagagatga tactcgatca gtacgctcca ccagcccggc ttgtccatgc
     3781 aggccgaact gaaccggctg acgccgtgca gccacaacgg caccagaatc aagacgggca
     3841 gggccagggc cacgcacact tctttggtcg ggccgccgac agccagggca aacgcggcca
     3901 gtgtcaggct gaacatcaac agaagaacga aggcgatgca cagcgtcggc caggtctcca
     3961 tacggcgcaa ctgatcggtg atcgcctcag ccggcagtgt catcgcgctg agggggctga
     4021 acagtccgca ctccgggcat cggcgtgggt cgccggacaa gccgcggagg ttgtatccgc
     4081 aatgcaggca gtaaaggtcc acgtcggtca ggtcaccggg gggtgcggcc ttgctgtcgg
     4141 actgcatgtt gctcgaagtc tgggctgcca cgtcgccggc ggcgcaagac tctgttttcg
     4201 cttgatcctg gcccattgcc cttgtcctct caacaccaac cggccgcagc tttctatgaa
     4261 cgccgtcgtg acagccgctc atagagctgc actttggccc gcgttgtggc aacctcccgc
     4321 tgtagcacat gtctcttcgc gttacactgc tgtcgggctc gtcgggccaa tagaaacact
     4381 gacagtccta ttcctgcgag cgctaccaag ccgattgctc caatgaacga acgctcctgg
     4441 cggacaagcc tgtggaaaac ccagatgctt gccgcagcag ccgaagccaa caagatcgag
     4501 agggccatgc cgtagatgtg gtattgaaca aagacactct tccaaccgcg cttgtacagg
     4561 cagtcagaac gaaactgctc catgccggtg aaccagagcg gtaacaggac aagcaatggc
     4621 agccaacaac cgatgcagag tccgagaggc atgcgtgaat tcaccagagc aacaactatg
     4681 catgcctggc ttccgaccag aagagttgcg gccgcgacac atgcggtcgg ccatgtttcc
     4741 atccgcccca gttcgcgggc aatcatattc gccgaggggc tgagggggct gaacagtccg
     4801 cactccgggc atcggcgtgg gtcgccggac aagccgcgga ggttgtatcc gcaatgcagg
     4861 cagtaaaggt ccacgtcggt caggtcaccg gggggtgcgg ccttgctgtc ggactgcatg
     4921 ttgctcgaag tctgggctgc cacgtcgccg gcggcgcaag actctgtttt cgcttgatcc
     4981 tggcccattg cccttgtcct ctcaacacca accggccgca tcgcggcaga tgaagatgat
     5041 gatctcctgt tcccggtgat ccggcaagaa cagcctggcc gacggccttg gcggcaaatg
     5101 cgtgacacct tgggcggggg cgcacagtgg gttccgcggg cggttgtttt tgacgtgcaa
     5161 ccccgcgcgg tatatcagcg tcgatgcacg ggcagcctga tgggacgaat ccgccgatca
     5221 gtgtgccgcc tgcgcgaacg gcaatgatcc ggtgttccga taaagagaac ggatcgtcgg
     5281 cgccggcggg ttgctcactt tcccatcggc cgttcgatca ggtcggtctg tcgcaccgcc
     5341 cgggttttgg ggttgacccg cagggtcttg acctcaaacg ggccgaggtt gaccctggtc
     5401 ttgaagccga tgcacggcaa agaaagcgtg gtcgaacgag cgtgaccggt cggctcgaaa
     5461 agccggacga tcaggtcatc gttatcctca gccagcttga ggcaactcat ctgaacaacg
     5521 tggtcactga gcaccgcacc ggccttgggc ctgggccctt ccccagaggg gaagaaggac
     5581 aatgcgtacg gcttctcgtt gtggacgagg gcctcgcgat cgacggccga gagccgctcg
     5641 tccagcgacc cggcgttcaa ccagaacctg aaaatccgct cgccctgatc gatgcgaggc
     5701 gtgtagcggt cctgctcgac gatcggccgg tcgaaaaacg ggtgcccggc gtacgccggc
     5761 gaacgcagca gcgtcaggcg gaggccgttt ttggagaagt cggcgccata ggaaccctcg
     5821 ttaatgcagg ttatcgccgt ccgcgcccgg cgagacgcga cggcgaccca cttctgcgcc
     5881 accgcctcat taccatgcat cggcaaaggg ttgataccga aggcggtctg cccgaggaac
     5941 tcaaagcccg ttccgcgcac cggaatcgag agcttgagca tgcgatcttt ctcgttccag
     6001 tgaacgcgga gttcaatctc gatttccgtg ccctgcttgg gcagcttgta acgctggcag
     6061 atgaaggagt cgttatacga cagcatgacc tcgacgacgc ttcgcacggg gccgtcctcg
     6121 atcacgcgga ccggaggcag ttcgtcgcat cgcacgccgg agaattgagc gcttttcttt
     6181 gccgacatga gcttaaaccg ccccgccacc ttgcggtagg cgcggttgag catgccccag
     6241 ggatcttcat tatccgccat caccagcggc tcgaaagcat tcttctccag gtagtcgatg
     6301 ccatggacct tgaggcaatc cacaaagccc gtcttcgcat tgatgacgac ctcgatatcc
     6361 tcggaccaga aggccacccg accgccgcgc accttgagct tcggaaccgg cttggcctgt
     6421 acaacctttt cgatcctgca atcgaagcgg ttcatctgac tcggatcgag ttccgccagg
     6481 aaagccactc tcttgcgcca gtcgagattc aggttgctcg cttctttttc gatctgcgaa
     6541 gggatcgcct ggccgccgcg gcggacgccc agcagagtcc atgtgccggc ccagttcgcg
     6601 tcggccaact ggaactcgca ttcgacaacg gtcctgacct tgaacgggtg agggttgtag
     6661 accaggatcg ggatttgccc gcccctggcc ctgggctgcc cggaggccag ggcaaagaac
     6721 gtccgtgcct tgacgcggga cagctcctcg agggcgtggt cgaaaagccg aagcgacgcg
     6781 ttctccaccg gttcgatgga cgaccccggc aggatgtcgt gaaactcgca gatcaacagg
     6841 tctcgcatcg cccggtgcag ctcctcccgc ggatactcca tcaaaccctg gcaggcggcg
     6901 gtggtggcca ttttctcaac caggtagagc tggttctcga gctcgcggtg cttctgtttg
     6961 atgcgcacct gcgtggtata gcagcccacg ccccaggggt tgaggtctcg cttgtggcgt
     7021 ggaagcaggt cgtattgggc cttcatctcg gcgaagaagg ccgcggtatt ggagtggagg
     7081 atgttgaatt ctttcgtgtt acggatcagg ttcgtgattt ccttgaggtc ttcacgagaa
     7141 ggccctccgc catgatttcc cacgccccac agcaacaggc tgaaaggcct gtcggggtgg
     7201 ttgttcatcc gctccatgat cttgaggtgg gccttgccga gctgcgagct gtaccagccg
     7261 tcgacacgac aggccatcac ctcggagccg tcgcaaccga cccagacgaa atggctgtcg
     7321 ggcaacgggc aatcgttctg gaacggtcgg cagaacaggt aggaatcgta gccgctcttg
     7381 gccaggatct gcggcagacc ccaggtgtgg ccgaacggat ccaggttgat ggccgtggtc
     7441 acgtcgacgc cgaacttctc cttgaaatat cgcttccctg tcaggatctg ccgtacgaag
     7501 gattccccgc tgggcatgtt acagtcgggc tgaagatacc agccgcccat gatgtgccac
     7561 ctgccttctc gaacaagtct gcgaatccgc tcgaacagag aaggctcgta ttcctcgacc
     7621 cactgataaa ggaccgcttc attgtgattg aagacaaacc cctcgaattc ttcgcagagg
     7681 tcggcggccg tccgaaaagt tgagattgta gcggccgctc cctcttccca ttcccacaac
     7741 cagaccggat cgagatgcgc gttgcaaagc agatggatgg tctttttcat ataacccagc
     7801 tctcactgcc cttatgattc ccggcaacgt cgctgcgccg ggaaagaagc gatctgagtc
     7861 taacgatccc gagcgggatg ggcaaaggta caaccgcctt gcctcgttgc ggggcgaccc
     7921 gcacacggct agattggccg gagttcagtg ggacctactt acagtacaga tcggaggatt
     7981 ccatgtctcg taaatggtct gttttgactg ccggcgtcct gtttgtcggg tccggggcgg
     8041 tgtggggggt ggaattcaag gatcaaatcg ccggcgacgc gatctccggc tgggagatcc
     8101 ggaacggccc gccggattcg tggtcggtca aagacggcgt gttgcattgc cacggggctg
     8161 agaaatgggg cggctggctc ggcaccgtcc gcaactatac ggacttcatc atcgaactcg
     8221 agtacaagct gagtccgggc ggcaacagcg gcgtctttct gcgcacgccg cgggagggtc
     8281 atccctcggc ggtctcattt gaaattcaga tcctggacaa cacggccgag aagcacaaga
     8341 acctcaagcc cgcccagtac tgcggttcga tctacaagat cgtcgcggcc gacaaggatg
     8401 ccaccaagcc ggtcggcgaa tggaaccgga tgcggatcat ggcggaggcc gaccacatcg
     8461 tggtgtggct caacggcgag aaagtcgtcg acgccacggg caagtcggac cctgagatct
     8521 tacaacgatc ccgcgaagga gccatcggcc tccagaatca ccgcagcgag gtttccttcc
     8581 gcaatatccg catcgccgac ctggccgagg accgggcccg gcgcaccaag tggtggcggg
     8641 aagcccgctt cgggatgttc atccactggg ggccggtcag cctcaaaggc acggagatcg
     8701 gctggtcgcg gggggggcag cgtcgcgggc acggcgaggg caggggcgaa ataccggtcg
     8761 aggtttacga caacctgtac aaggaattca acccgacgag gttcgacgcc cggcagtggg
     8821 tgcagattgc gcgggacgcc ggaatgaagt acatggtctt caccacgaag catcacgacg
     8881 gcttttgcga gttcgacagc aaactgactg attacaagat cacccactcg cccttcaaac
     8941 gcgatgtggt ggcggagctg accgccgcct gccacgaggc cggcatgccc atcggcttct
     9001 actattcgcc gcccgactgg caccatcccg actaccgcac cgagaaccac ggccggtacg
     9061 tcgagtacat gcacggtcag atccgcgagt tgctgagcaa ttacggcaga atcgcgatca
     9121 tgtggttcga cgggctcggg ggaacctcaa aggactggaa atccacggag atgttcgagg
     9181 acatcttccg gctgcaaccg gacatcatca tcaacaaccg cgccggcctg gccgccgatc
     9241 atgacacccc cgaacagcag atcggcaagt tccagaacaa ccgcctgtgg gaaagctgca
     9301 tcaccatttg ccggcagtgg gcatggaagc ccgaggacga gatgaagtcg ttgaaggatt
     9361 gtatccggac gctggtgcga tgtgccggcg gcgacggcaa cctgttattc aacgtcgggc
     9421 cgatgcccac cggggaaatc gaacccaggc aggtcgagcg gctgagggaa atgggccaat
     9481 ggctgagcaa atacggggag agcatttatg gtacgcgcgg cggcccgttc aagcccggga
     9541 tctggggcgc cagcacgcat aaggacaaca ggatctatgt ccacgtgttc gaatggccgg
     9601 ccgacggtca gtctcttgcg ttgccgccga tcgggaaaaa ggtgctcggc gcagccgtcc
     9661 ttaccggcgg tgcggccgag gtcaagcaaa gcagcgaggg cctggtcatt accatgcccg
     9721 cctcggaacg tcaggaggtt gatacgatca tcgcgatcga tcttgacggc ccggccgcgg
     9781 aaatagcccc tctggccaca aggctcggct cccttgcggc gcacaagaaa tcgactgctt
     9841 cgaacgtgtt ccagaagagc tggctccacg gtcctgagaa ggccttcgac gatgatgaca
     9901 gtacccgttg ggccaccgat gccggaacaa agcaggcatg gctggaagtt gatctcggcg
     9961 agcctaccac catcggccgg gccgtgatca gcgaagcatt tgaccgggtg agggcgttcg
    10021 aattgcagta tcgcgacggg caggagtggc gtaccttcgc ccgcgggagc accatcggag
    10081 ccgacaagct gatcactttc gctccggtga ccgccgatcg ggttcgcctg aacatcaccg
    10141 aggcaacgga gggcccgacg atatgggagt tccaactgtt cgccccgtcc aagtgagcag
    10201 agggagcgat tgccccgccg cactcttgaa cgctcagtgc accgcgatgg agacggcatg
    10261 tctcaaaaga ggatccagat cgctgctcac aagcccagcc cctggattct ccgccctcgg
    10321 ccggtggttt cgccaagact gcggttgttt tgcttcgctc acgccggcgg cggtgcctcg
    10381 ctgtttcgca actggccttg tggtttgtcc gacaaggtgg aggtctgtgc ggtccaatct
    10441 cccggccgtg aggagcgact cgacgactgt ccttatgcac gtttcgacgc cttggaacag
    10501 acaacggctg accagatcaa accctggctc gatgtgccgt tcgctttgtt cggccatgaa
    10561 ctcggggcac tggttgcctt tgagcttgta cgacgacttc ggcgaaccgg cctgccggct
    10621 ccggttcgat tgatcgtggc aggctgcgcc gcaccccact tgccaagatc aaggcccccg
    10681 attcacaggt tgtccgacga cgagtttctg gatggtttgg cgggcggtct ggcggcggtt
    10741 cccgacgccg tgcggcgcag gcccgacctt gtggcccgca tccttcccgg cctgcgagcg
    10801 gatttcacca tgctggaaac gtatacgtat cgcccggagc atcgcctcga ctgcccaatt
    10861 accgccctgg gcggcagcgc tgaccccacg atgccgaccg atgccttggc cgcctgggcc
    10921 gagcacacga gcacgttctc gcagcaaatg atccagggcg accagttctt tctcttcacg
    10981 ggtgaaaccg aagtgctgtc ctgcctggcg aacctgcttt cgccagacta tctgtgaaca
    11041 gccggcgtgc acccgccaca ttgggccgac gaacccgttg ggcccgaggc ctcggacgat
    11101 gatgttctct tggccgcgct tcgccgcaag cggcgtcact gaccacaggt gccccttggc
    11161 gaaggcatga cccgaacccg attcgaggta tcgctcgaag gcctcggctt tccagtcatt
    11221 ctcgacccgg acgaccgcaa cggcctacca tgggacgtgg gctcgggtat accgagactt
    11281 gctcggattg tggtctgaca atcgttgtat gaagtcggtg gtcggaccga tatagcgctc
    11341 acccggacgc gaaaggcttt caagcaggca gacatgcttg acaggtcgta ctccaccccg
    11401 cgccggccct gcttcgcgtc gcttgccagt gctacacagg acgttctgcg cagcgcccgc
    11461 atcgggtcga ccaatgcggg aacgacgcag aacggaggcg gagggattcg aacccccggt
    11521 acccttgcgg gcacaacggt tttcaaaacc gtcccgatca gccgctccgg cacgcctccg
    11581 ggaatcggcg gtcatgtgcg agatcattct acgacaaatg cgggctgaag ggtagtgccg
    11641 tgccggcggc aaatcgactg ctcagagaga ccagagacgt tcaggggggc gccgcatcgc
    11701 cggtttcctt ttcgtgctgt cttgtgtttc tcgttgctat ctacgccttt tgctcctcag
    11761 gtgaagatgc agacgggtga tggccacaaa aaacgcaaga agtcacaaag aacgcttcgc
    11821 ggcgtgttcc acctctggct gacagccaag agctgttagc ttccttcaca gcaccttggt
    11881 cttgcccggc acggccaccg ggtagcggcc ctgggcgtcc ggcacgatgg gggggcgagt
    11941 ccatggtgta gctctccagg cccggagcga ggacgaagtt cgacgccaag acgagcccac
    12001 acaggtcatg gagaatgctc tgaccactcc cggcgtggcc cgtccagtag cagtgtgatg
    12061 tgattcagga tgtctcgacc cagaatacct cgttcttcct caaccaagag atagcgacct
    12121 cggaaggcac gtctcaggaa aaccaagtcc agcatgacga cagaagcgac acacctccta
    12181 ccgtcgaatc ctgccaattc gcagtgctcg ccggcgatcg gcatcacgcc gagttgctca
    12241 accgcttttc ggggcaacaa ggtaacgtct gctcctgtgt ccatcagtag ggttacgccg
    12301 gaaatccttg cgccgctttc cgggttccgt agcacgacct ccgcgacggg agccggcgga
    12361 tggaagcgcg ctgcgtcata gcccggcatc gtcgctcacc tcgacgacat gcttgacaaa
    12421 atcggcagcc tgttctggat gcgccaagtg cggactatag acacgtgcct gcggtggggt
    12481 ctcgatatcg ggaaccacga cgtagacctt cgtgttgtcg ggcagcatca cgttgtcacg
    12541 gagccggatg cagccgtttt cgacaattcc ttcaatcgtc gagacagcca tgttgcacct
    12601 catgtcactt gacgacgctg acggagccat cagtcgctcg cagcacgccg gactcgcccg
    12661 ctcgcccgtc gggcaagcca atccctcaag ccttgctcca tgggtggtct gcctccaaac
    12721 cagcctgtcg cttgtggggc gattcggcca aaccaaggaa gcccggggag gcgcggagca
    12781 tcgctctcca actcattcta cgtcgtgatg aggctgcgat gcaaaccggt tctgccggat
    12841 cgggtccgtc acagttcagg cggggcgacg ctatggcttt tcagaaccgc gactgtaagg
    12901 taccggccgc cgtgcggtgt tggccgcttt gtggctggcg ctacccaccg actggcctgc
    12961 ttggtccctc gctcagtatg cagcagtgaa tcgaccgcgt tcacagcacc ttggtcttgc
    13021 ccggcacggc caccgggtag cggccctggg cgtccggaac gatcggcggg gtcgagtcca
    13081 tggtgtagct ctccaggccc ggagcgagga cgagatctga cgcgagggcc tgctcccagg
    13141 tgatctcctg gcccgactcg gcggccatgc ggccgagaat accggtcagc gtggcataag
    13201 cgcaacgctt cgtttcgttg tacggcttgt tctgccggat ggcctcgaag agtcgctcgt
    13261 gctcgacctg atactgatcg ctcttctcgc cctggtaccg ccagatgatc tgatcctcag
    13321 ttcgcctgtg ccccttgtag atcagggggt cgggaattcc ctcgcccaga acggccgagc
    13381 ctttcgtccc gtgaatcacg ttgccccaga agccccagca gtcggccatg tgacgagcct
    13441 gggcgaacaa ccgcgtcccg tcggcaaacg aatactcgac ggcgtagtgg tcgaacatct
    13501 ggtcggcttc ggtgcgagga taccgtccgc cttgcccctg agcggagacc ggccaggcat
    13561 ccttgaccca gcagcacacg tccagattgt gaattagcca gtccagaatg aaactgccgc
    13621 ccagccagtt gaagttgtgg aagtggcgaa tctggtaggc tatttcggtc atgtccgccg
    13681 gcttgggcgt gatgcccact ggtccgtgct cgcggtagga ccagcaggtg atgacctcgc
    13741 cgatgatacc gtcgtggatc cggcgaacgg cctcctccaa gggcgcggaa tgacggctca
    13801 tcagcccgcc agcgatcttg atgttcttct tgtccgcctc ctcgccggcc ttgaggactc
    13861 gtcgaactcc ggggccgtcg acggcgaaag ccttctccat aaacacgttg cagcccttct
    13921 cgacggcgta ctcaagatgc ggcggccgaa agcccggagg cgtcgccaga atcaccacgc
    13981 ccgatccgtc caggctgtcg atggctttct tgtatgcgtc gaagccgatg aattgacgtt
    14041 ctttcggtac gtcgatctgc ttgccaaatt gtgattggag cttctggaga ctcgattgca
    14101 agcgatcgtc gaacacatcg gccatggcca ccagccgggt cggcccacta ctggtggaaa
    14161 gcgcattgat ggcggccccc gtgccccggc cgccacagcc gacaagagcg accttgaggg
    14221 tattgccctc cccggcatgg acggccggga tggccgtcaa gcccagggct gacgccgcgg
    14281 cgatgcggcc ggaggtgctc agaaactcac gacgcgacgc tcgggactgt ttgtcttgac
    14341 tcatggttcc tccctcggat ggaccatcct gatgtgggta ccggccttac agtctcaccc
    14401 ggtcacgccc cgggccagcc aagtgaatta tcagtgatcg cctccggctg aacaagatct
    14461 cagctcgttc acgcaactgc ggatgtgtgc gccaatcttg agacccttgc gccagcagta
    14521 tacgatgcca gcttgaggcc agcagaggag gtcgaccgtg accagcaggg aacgggtcgt
    14581 cgcggcactg aatcaccggc agccggattc tgtcccgata gatttcggcg gaacggcggt
    14641 gaccgggatg cacgtcacgt gcgtggctgc cttgcgcgat cattacggcc tggaaaagcg
    14701 gcttgtgaag gtccacgaac catatcagat gcttggttgg atcgatgacg acctcgcgga
    14761 cgcaatcggg atcgacgtgg ccggcgtgat cgggccgacc accctgttcg gcttcgccaa
    14821 caccaattgg acaccctggc gggctccgtt cgggctggag gtgctggtct ccgaacactt
    14881 caagaccacg atggacgcca agggcgacgt gtttatctat ccccagggtg acaccacggc
    14941 gccgccaagc ggccgtatgc ccgatggggg ctacttcttc gacaccatcg tccggcagga
    15001 accgatcgac gaagacaagc tcgatccgca ggacaacctg gaggagttcg gaccgatcag
    15061 cgaggccgac ctggcccact tccagggtga ggtgaagcgg gcatcggcca cggggcgggc
    15121 ggtcatcgcc actttcggcg ggacggcctt cggcgacatc gccctggtgc cggccccgtt
    15181 tctcaagtac cccaagggca tccgcgacat cgaggagtgg tacgtatcga ccgtcgcccg
    15241 ccgcgactac gttcatcaag tcttcgacag gcaatgcgaa atcgccctgg ccaacctggc
    15301 gaagatccac gcccgcgtgg gcgatgcggt cgacgcggtg tttatctgcg gcacggactt
    15361 cggcacgcag acctcggcct tctgctcgcg agagacattc cgcgatcttt acatgcccta
    15421 ctacaggcgg gtgaacgatt ggatccacgc ccacacgaaa tggaagacgt tcaagcactc
    15481 ctgcggggcg gtggagcagt tcctcgggcc gttcatcgag tccggcttcg acattgtcaa
    15541 tccggtgcaa tgctcggcgt ccggcatgga cccggcacat ctcaagcgaa cctacggcga
    15601 cgagctgaca ttctggggcg gcggcgtgga cacacagaag accctgcctt tcggcacgcc
    15661 ggcgcaggtg cgcgagcagg tgctgtcgcg atgcgagatc ttttcgaaaa acggcgggtt
    15721 cgtgttcaac gccatccaca acgtccaggc ccgcacgccg gtcgcgaaca tcgtggccat
    15781 gatcgaggcc gtccacgaat tcaacggtcg gcggtgaatt ccggtgttcc gtccgccacg
    15841 gaacacggct cacccaggcc tatcaccttg gtaccgcaaa tacctcctaa gtcatccatc
    15901 ggccagaact gaacccggac ggagaatcgg ttgcggatca tgtgcccggc tcggtcatcg
    15961 ttgtcggccg acttgccggc gcgctggaag tatcggtcgg ggccgtcgcg gtggtcggcg
    16021 ggaagatggc ggtggggcca ctggccagca atcgctggag cattgcgtta cccggatggc
    16081 ttttcgacag ttcttccacg agggccatga cgaggttttc atcgcgagtg acgccggcaa
    16141 gcaatcgcag gtactgcaag ccggaggagt cgtctttctg ctcgaacgaa aggcgaatca
    16201 ccgccttgac gtgttccaca acagcgacat catcttgtcg ctcggccgca cgccgggccg
    16261 ccagatcatg ggttgttcgg ttgcccggat ctaacgccaa agcccgctgc agccacttgt
    16321 cgtacgaggc ggcgcgagcc tcgatcggga acgacgcgaa cgactggcac aggtcgacgt
    16381 agccgtcgct gagccgttga tcgatctcat ccgacgcggc ttcgggatac agcttgccga
    16441 ggagttcgcg tgcttccttc tcgctatcgg ccgcaagcat gtacatggcc cgggtctgca
    16501 tcaccggccg aagctgaagc tcgcgctccc ggacgatcgg ccattccttg attgcctgat
    16561 cgcacgccgc cacggcttcg gccggccttt gggcaagcat cagatatcgc gactgatcga
    16621 gcctcgcgtt cgaaaccgcc cctgggaagc gtccccccag ttgcctcgac agctccgccg
    16681 cctggcctga cagctccgcc gcctcgagaa aacgcccgga cagcttgctg acccgggccg
    16741 acagtcgcaa cgattcaggg gcgtaaggat ccggccagtc atcggcacta ttcgcggcca
    16801 tcgcctgccg ggcgtccgcg agcatcttct ccatcgcctc ggagaagccc ttctcgttga
    16861 tcatggcccc gatgagcggt tccatatccg ccagccagtc aataccgaaa cggtcagtct
    16921 gacgcaccgg cacgggtccg cgtctcagcg gccgtcgcaa caggttcagc cgataggcca
    16981 cggactcgtc gccgcacagc tcaaaaagcc tcatggcgac cgccatgtcc tgcgggtcgc
    17041 gactgagttc aaccgacaac aacgtgcgaa cggctgcggt gtagtcccgg acgtccgccc
    17101 tcagaccggc ctgtcgttcg atcatctcga cctgctggcg gtacacttga agatgcgcca
    17161 ggatccaagc gattcgaggg tacccgggaa tcacaaccag aagcgtgttg cccgtcgcct
    17221 cgcaaatcga cgcgaaacga ttgaacgctt ccacgtcttc gcggatcatg tcgaccagac
    17281 ccgccggagg ctcggcatct tgcgggaggc gcgccgctcc gcgcatgcat cgatcgacgc
    17341 tgccggcggc ggcgatcagc gagacatgag tggtgccgtc gtacgcctcg acgtagtctt
    17401 cgacggccag ccggccggat gatgcgatcc gggccgcgtt catcgaggca tcccactcct
    17461 gctcttcggc gcgggtcagg atctgatatg cagcgagcgc tccttcccaa ttacgccata
    17521 cggtccatgc gatcccgagg gaggcggcga tcccggcgat gaggcccgca acccgaagag
    17581 gcgtgggctg ggagctgatg atcggctctc tcgcggcggg cagtctcagc gagagcgccc
    17641 acgtcagacc cgccgtcgtg tagaagacgg ccggcaggcc cggcttgcgc agagcgacat
    17701 cgccgagttc ttcgattacg acggccgtga acgccgccag cgctccggcc agcagccact
    17761 ggtccagcgg cgtcttgacg cgggaccagg ctcgaatggc gccccacatc gtgagaacca
    17821 gaaaagccgc gcacaagacc gaccccacca gtcccgtctc ggccgcgatc tgaaggtact
    17881 cgttgtgagc gttctcgatg actggggcca taaatgccgc ggaatccttc tccatatccg
    17941 gcgcctgcat tgcctgggcg agcaacatgt agctgccctg tccgtggccg acgatgggag
    18001 ccgtcgaaaa aagctgaatc gcgtatttgc cagcatagag acgaaaacga atggtcgcgt
    18061 cgcggcccgt cgtcccggag gtgaccgccg accagttcag ggcgaaaaac agcgccgcag
    18121 cgcccacggc gccgaccggc agcaaccatc gcccgagcct tcccaggccg agccacaccg
    18181 ccaccgccgc accggcgacc aaagcccatt gtgccgtccg cgactcggtc aaccgaagcg
    18241 cccacatcat gaccgcaacg ccgacgacgg caccgatcgc cacccaccag ccgcccatgt
    18301 cgcggatgac ttccgcgaac atccgagcgg gcgccgtttt ccgctcgcgc gaacgatcca
    18361 tggtctcggc cagcaccccg aaaacaatcg cgcctgcgag gcacagtccg acaatcaggc
    18421 aggcggccat gaacagcggg ttaccgatgg gaaactccag ccgcataaag gggcttcgtt
    18481 cgtgtcggta ccagattccc agcgccgctg tcgccgtcag gatggccgtc atggcgaccg
    18541 ccactccgcg cgctcccgcc cgggtcaacg tgcgccccag aatcagcgcc caaatggtcc
    18601 agatggcctg ccgactgccc tccccgaggc tggcctccgg ccacggagac cattgcgagc
    18661 tgatcaccat ccacagggtc agcagcacca aggcaatctg ggccaatgcg actgtggagc
    18721 aacagcgcct gccggccggg gccggcgtcg gttcctcgac cgttatcggc cgggtgacgt
    18781 cgctgcgcgt aaaccacatc agcgtcacgg ccagaacggc ggccgccacc ccaagcccgt
    18841 gggccaacca cttgacctct gaaccgctgg aggtcgggaa ggcatagttg aagttgagca
    18901 gatcgacgat gttccgcagg accgagccgg gggcccactc ctgatatccc gtcttgcttc
    18961 cgtgggtggc gctgatcaac agacaaaccg acgccaggct gacggcaacg attgcgatca
    19021 tgatccgatc gaacgtagtg gttccgggac gaccgttgcc gcccgaagcg gccgtcggtt
    19081 ctggcatcat acgttgggcc tccaggggat ctcggaacgt ccggcttgcc ggttggccag
    19141 ccgcgtccag gcgtacaaca ggtcgctgag acggttgaga taagccagca gttcagctcg
    19201 aaccggcgaa actcggtgga gggtcacgat gctgcgctcg gcccgccggc aacagacccg
    19261 ggccatgtgc agccggcagg ccgcctcggt tccacccggt aagacgaaac aaccgacgga
    19321 gccggcggcc gcctcagcct cgttgatcca tgattccagc ctccggcagt ggtcctgggt
    19381 gatcgccaga tgcttccact tgtcaccggc cgtcggagga gtggccagct ctgagcagac
    19441 atgaaagagc tggctctgta tgtcgacgat ggattgggca atcgcgccct cgcatgcggc
    19501 ccgacaccac ccgagaaccg aattcagctc gtccacattg ccgtaggcgc atacgcgagg
    19561 atcatctttg gccacccgac tgccgtcaac cagcgaagtc tcgcccatat cgcccgttct
    19621 cgtgtggagg ctcatgttga tcgccccttg gcagcaaccg tttgcgatgg gcattatagc
    19681 ccgcggcggg aattgcgaca tcaccgtgga agcggcgtcg cggttctcag agcgtgtgtg
    19741 agaagcaacg gggccggccg ggcagtatca ctcagccagg agcggcaggt ctccgccgcc
    19801 gtcggcggct ccgaccatgc cctacccttg tcacgcgcgc tctgggtcgc cgatgtcgat
    19861 gagatcaggc cgactgcctg acaacttgtc ccccgatggg cgacgaaacc gccccggctt
    19921 tcagtcgcgc cacctgacgt ttcttcatcg tcaggcggag accaccggcg gcttgcctgc
    19981 cacagcgcag ccttttggcg aagacggggc aaaccgatgc gtgtaactca agtgccgaaa
    20041 acggcaccgg cgcaaacaaa aaccggcttc gggccggttg tcaggcgtct gattcctgca
    20101 aggtcagttc gccgaaattc gagcgatggc aggcgggttg ccgaactcct ggacccaata
    20161 cacgccgtac tcgccgccca cccgcacggc gatcccgatt tccttccact gcggggatag
    20221 aatgttctct cgatgacctt cggtcgattt catccagtcg gccattgcct gctcgggtgt
    20281 ggtctgccct actgccagat tctctccgac ggccagaaac acataaccgg cactcactgc
    20341 acgctgtccg gggccttcgc cggtggggct caggtgcgag aagaatccgc acgcgatcat
    20401 ttccttggcg tagtcgtctg ccatctggct caggaccgga ttcaaggtca ggggctgcag
    20461 gccgcggctg gtccgctcgt tgtttaccag ttcaagcatg cgggcaacca ggacgtccgc
    20521 attggacggt gtgtagcccc cttccggcaa ggactcggta ttggtcccca tcccggcaac
    20581 ctgggttccc gtgccgaagt cagaggcact ggggcagcct gccaggaata ccgacccaat
    20641 cgacagggcc agttggatcg ttagtgtaag tgaggacccg gctttcactc ggtttgctcc
    20701 cgtcggtctg gcgatgtcca caggcggctt atggcaatct tcgcttcaac ccctccgccc
    20761 gcaccccttt ggctacccgg tggcgggaca tcacgccctt gcttaatgat attggttcat
    20821 cggccaagca tcaaacaaaa tctaacaggt tcaccctcag acggtttgcc atcaggtcgt
    20881 ttttgggctc caggggcgag gtctgcccct cggaatcaca cataaggctc gcccacaaaa
    20941 caacttatcg gacgagtgct ttaacaccct tgtcacccgt tagacacccg aaaacaagaa
    21001 aggtttgcag cggttttttt cggctgaggc tgacaagccc ctgtcggcca attgatctgc
    21061 tgctcctcgc gtcgcagacc cccgacggag acggtcctcg gcggtaccgt tcgccgtccc
    21121 ggtcagttca attggaggat gacattgaca aacgattcca gccagtccga ccggatctcc
    21181 cacctcacgc cgaccagcca aggcaaccag aaaatcatga ccgccagcag caccatcatc
    21241 tgcgacgcca gcaccgtcag aaacgggcgg tcaaaccgca gataacggac atacgcgtaa
    21301 tagaggcggc gggcgcagaa cacgaccgac aggaaaggca ggcacagcag gaaaggttca
    21361 tgccagcctg tgttgctgcc ggagatgacc tgcccgatca gaagcaacag agccagaagc
    21421 atcgtcaagg cgggcgggat catgtcgaag ctgtagagca cacatcgccg cacgtgcacc
    21481 ttgttgactt tcgcccggcg catcgaccag cggaaaacct tcagagaaac cagggtcagc
    21541 aagggccaga caaacggtag catcgcagcc atccagagca gcttctccgc acgattgcac
    21601 tcccatgtga gtgatgagag gaactcccag atcaccgaag gcagccgggt cgcgccgggg
    21661 acgaagacgg caggcggcct gccccagccc cacaagccac tgaacaaaga gaacggcgag
    21721 gcttgtcgtc cttcgatgag gttccaggca agctcgcaga cgtttgtcgg gaacagtatg
    21781 agtattgcca cccctgcgaa gcacaaaagc gttgccagcc aataccccag aagtcgtcca
    21841 ggttgcgagg gcatcaccgg atgcagcgag gtccagaatc gcttcggccg catcgcccct
    21901 cgcaccgtct tgaggaagga ccagaagttg cgacgaggat ggtgttcgaa gaggtacggg
    21961 tgttcgcggc ggtttggatc cagtacctct ttccatgcga agcgatagcc gcactccggg
    22021 catcgcggca cgttcaaccc gcgaaggttg tactcgcaca gcgggcaatg gacgtcccag
    22081 tcaatcgttg accagtcggg cggaggggag caggcgtcgg cgggtaccgg tcgggcggcg
    22141 gccgtctcgt cgtggcacga tgtgctggtt aagccatgaa atgctggggg ttcttccacg
    22201 gcgatcaatc cggaatacgg ccgagcctgt tgctcaggcg acacgtgtgg gttgtaaccg
    22261 agcctggacg gggcggcaag cccggcgagc ccgtggccca ccggcgcggc cggaactccg
    22321 ttgaagtgga cgattcatca agtgcgaggt agaaatagtt atatgcttca aggaggtctg
    22381 tatgaccatc gagcagcttc gagcagtcca gcaggcgacc ccttttcgcc cctacgtcct
    22441 ccagccggct gatggcgacc ggattgtcgt gcggcatccg gaactggtgt tgatcacgcc
    22501 gggaggcaga accatagccg tcgcaaccgc cgctgatgcc gtcaagatta tcgatttgtc
    22561 gctggttact gccattgata tccccaagaa ccatcgccga actcgggcac tcagacaaac
    22621 ccgatgacca gcacatcggc cggcacgccg tttgcgtccg cctgcgactg cctatataaa
    22681 cctgggcggt gcacacggca acggcaacgc tgggggaatg aagaatggtg aatgcagaat
    22741 tggaaatggg gcttgttccg tcgagaacgg cgaaacgcac cggaggcggc gtttcggtgc
    22801 aagccgagag agtcgagaag caggccgtct ccagggctcc atccggttgc tcctgttcgg
    22861 attttgtgca actgaagggt agactaacgg attgaacgat gtcaggtgtc gaccgaaacg
    22921 gcctgccccc gcagagccgt tggtgcggca ctctgcatgg atttcagatt cagtgagtaa
    22981 cggactgacg gaccaaaaga ctaaagggct aataaaccaa ccaactcatg agtctcgtca
    23041 tcattggtac cggcaactgg ggatccacgc tggcgggctt gctcaacccc gagcagccgc
    23101 tgcggatgtg gtgcgagcgg aagcagttca tcaagccgac cgtcgagtcg ctcaagcgcg
    23161 ctgccggggc cgatcggccc ggggtcgtcg tggaagagat gttcgcctcg ccgctgaccg
    23221 ccgatgacat tgtgctcgtg gtggtgccgt cggcccaagt tggtccagtg gctcagaaga
    23281 tccgcgagaa gatcaagcct ccttacccga tcattgtcag cgcctctaag ggcctcgagc
    23341 gagccacgtt tcgcacgtgc agccaggtga tcgcgtccat ccttccggag gcgaccatcg
    23401 ccgtgatgtc cggtccgaac atcgccaagg agatcgccgc cggccgcccg gccaaggcgg
    23461 tcctgggttg cgacaacgtc cacgtgctgc tcaaggtcgc caaggccctt cgcagcgacc
    23521 ggtttcatct ggacatgacc cgcgacaccg tggatgttga gctgtgcgcg gccatgaagg
    23581 gtgttttcgc cattggggcc ggggtcatcg atgcccgcaa gtggggctgc aacttcatgg
    23641 gcctgatgct gaccttcggc ctgcgcgaga tagccgaggt gggcaggttc ctgggcatct
    23701 ccagctctca cgtgttcggt atcgccggcc tgggtgacct ggtggccacc tgcctgagcc
    23761 ccgacagccg caactatcgg ctcggacaac tgctggccgg cgggacgccg ctcaaggagg
    23821 ctctctcgca agtcggcatg gtggtcgaag gagccatgac cgcctccgcc gtttcggaaa
    23881 tggctgcgct gaggctgcgc gtgccgttat tcagcgcgat ttcggcaatc gtggaagacc
    23941 cgtgcgaagc cgcgtttcag cggctcgaac aggtcctgct ggaatacccc agtaacggat
    24001 gaaagcgatt attcttgccg ccggtgcagg tcgtcgcctg ggtgcaatcg tgcccaagtg
    24061 cctgctggac gtcggcggaa tgagcatcat tcatcggcag cttgccgcgt tctcgactga
    24121 aggcatcaag gaatacgtgg tcgtggttgg ttaccagcag gatcgcgtgc gtgaacatct
    24181 ggccggtcag cccggccgat tcgtcttcgt cgtcaacgag caatacgcgt cgaccaatac
    24241 cgtctattcg ctttacctgg cggcgtcgta cctgacggag acatgctggt acgccaatgc
    24301 ggacgtggtg ttcgaccgga ggttgattcg ccggctcgcc gaggattcgt ccgccgcggc
    24361 cctggccgtt cagacacact cgtgcggcga ggaggaagtg aaggtcatcg tccgcgaggg
    24421 tctggtcgtt cggatcggca agacgatccc cccggccgag gccgccggtg agttcctggg
    24481 cgtcgcccgg ctgagcggca acgtcgccgc cgcactgagc aggacgctta ccgaactggt
    24541 cgagcagcag agcgtgatcg ccgactactt cgagcgcggg cttgaccgac tgtgcagcga
    24601 attcgcgatc aaggcgatcg acgtcaccga tctgccgtgc cacgagatcg actttccggt
    24661 cgatctcgag aaggcccggc gcgagattgc accgaggttg gaggaatagg gcagctatta
    24721 gccgtcggct attggctatt agccgtgaat ccgctcgttc cgggctgggg aagaccgaga
    24781 gcaagaaacg accgctggag ttgttttccg atgcttgcgt ttctagggca ggtcatcctg
    24841 gtttcgttaa gcggcgtgat ggccccgggc ccggtcacgg ccgcaacgtt gaccgccggt
    24901 acgcgcaatc gacattccgg cctgctgatc gccgtcgggc acggtatcgt cgagttcccg
    24961 ctgatgatcg tcatcgtcgc cggtgcgggg atgatcttca aacaaccggt cgctcagatc
    25021 atcatcggcc tggtcggggg ggcgtttctg ttgcacatgg gcgggcagat gctcgccggc
    25081 ctccgcaagc aggaagaaat cgccgcaccc tatgtcgaca gccatcccgt ccggatcggc
    25141 atcgtcctga cggccacgaa tccctatttc cttttctggt gggccacggt gggcctgggt
    25201 ctgagtctcg aagcccggaa actcggtccg actgcgttcg ccattttcgc agtcgtgcat
    25261 tggctatgcg acctggtctg gctcgagatc ctctcgcagg cgagcttccg cggcacgcag
    25321 atcctcggcc gcaagagcat gcccatcatc ctgggcatct gcgcgacggc tctgatcgtc
    25381 atcggatcgc gattcgttta cgatgcaggc aaggagatga tcatccaagt ctttccgcag
    25441 ccaaccgaaa catacatctt tcccgacacg gtcccggcct cggccccggc gaccctcccc
    25501 ataacccagc cggtctcgac cttggcaccc gtgtcgtgag cgtttgcgcc ggatcgccgg
    25561 ccacggatgc cgggcggctt tccggtcggc cgtcacagca gcgagaccgg gtcaacatcg
    25621 atcagaacat gtttgtcgga gaggggcaga atctcttcgc cgcgaagtcg ctcaagaatc
    25681 tgaaggagtc tggctgcatt ggccgcgcgg atgaggaagt caaaacgata ctgacctcgc
    25741 agccgggcga gcggagcgct ctgggggccc agcacatccg ccggcaagcc gtgtcgtttc
    25801 aggctttcgc ggattctctc tgccagttcg tccgcatgcc tggaggcctc agaccgcgcc
    25861 ggataggaca ccacgacgcg ggtcagacgc gcgaaggggg ggaagcctct gtttttgcgg
    25921 atggcaagct cgtggcgggc aaatgattcg tagtcatggt tggcggcgaa ctgaatcgcc
    25981 gggctcagcc cgaccaggct ttggacgacc acgcgtccgt ccgactcgct ccgcccggca
    26041 cggccggcga cctgcgtgac cagttgaaag gtccgctcgg ccgcccggaa atcaggctgc
    26101 aacagcgagg tatccgcatt gatcaccccg accaggcgaa cgcgcggaaa atcgagtcct
    26161 ttggcgacca tctgagtgcc gatcagcacg tcgagcctgc cgtcggcaaa gtctcgcagc
    26221 acgcgctcgt aggcgctgat cttcgtcatc gtgtcgctgt cggcgcgggc gacgcgggct
    26281 tgcggaaact tggtcctgac ctcttcctcg actcgctggg tacccatgcc gaaacgaacc
    26341 agcgtgccgt tgcaggaagg gtcgctgcag cgcgtcggca tcaccattct cgccgaacag
    26401 tgatggcaca gggctttgcc ggtcgactga tgaaacacca ggttaacgtg gcaatggggg
    26461 cacacgattg tcgtcctgca ccggctgcag acaagatagc tcgcatagcc gcgacggttc
    26521 agcagcagga ctgcctgctg tccggccccg agggtctcag ccagctcgtt ttccatctcc
    26581 tgcgacagca ggtgcaggcc cttgcgctgc cgttgctcga tctgcatgtc gacatactgc
    26641 accctcggca tcggcaggcc ggctacgcgc gtgggcagaa cgatcttttc aaaatgcgaa
    26701 agtcgttggc agttgtgcca catctccagc gagggcgtgg ctgatccgag gatgatcggg
    26761 caggagcata tctgtgcccg cttgatggcc acatcgcggg tgttgaaccg gggtgactgc
    26821 tgattcttga agctggcctc ctgctcctcg tccacgacaa tcaggcccag gttggggcag
    26881 ggggcgaaga ccgcgctgcg cgttccgatg atcacgcgtt tctctccggc ggcgatggcc
    26941 gcccaggtca gtgatcgctt cgcgccggtc agaccgctgt gaatcaccgc cacgcgttca
    27001 aaacgggcgg ccagacgctc caccagttgt gtggtcaacg ctatttccgg caccagcaga
    27061 acggcctgcc tgcccgcggc gaccaccgag cggatcgccc ggatgtagac ctccgtcttg
    27121 ccgctgccgc tgacgccgaa gagcaacacc gcacggaacg tcccctcagc ggtcagcgag
    27181 gcaatgcggg cgatggccgc ctgctgatcg acgttcaact cgaacgtcgg ctcggggccg
    27241 ggatgatcga aatccggcgc cggcgcggcc acacggcgag tctcctggct gatatgcccc
    27301 gttttcagga ggcccgcaat cacggccttg ccgacacccg tgcgggcaag caaatcatcg
    27361 aggtccagtc cctgtggggc ggcggccaga gcattcaaca ctgcctgctg tcgggccgag
    27421 aaccgcttcg gtcggccggg cggcggcact tcctgtgtct gctcggactg ggtgtgagaa
    27481 tgaccggaag cttgcggtag ggcagcgtcg aggtcgccgc cgtgaccgag acctgccgca
    27541 ttgtcgatgg gttggcaggt ctccgccgtc tctggcggct cggaccgtgc cctaccttcc
    27601 tccgccgcct ccgaccgcac cctaccctcc tttgccgcct ccgcagattc cacagcaccc
    27661 gtgctgctat tcgcgagcat gaggcgacga acggtgatga atcccctttg cctgcggatc
    27721 gcctccggga ccatggctga caaggctcgg ccaagcgggc agcagtagta gcgtgccatc
    27781 catttgccca gttccatcag cggaccggac agcagcgggg tatcgtcaat cacatccgcc
    27841 agcggccgga gcgtgctggt ccacgcctcg cgggcgacgg atacgcagaa ggcaggctgg
    27901 gggcgtcggt tgcgaccgaa gggcaccaga acgcgtttgc cgacggccac tttctcctcc
    27961 agctcaggcg gtatcgcata gctgtattgt ccttcgaccg ccgccaccat cgcgacgcgg
    28021 gccaccggcc cttcgcgcac ttcgggcgtc tcggtccaca gcgccggtcc tgcatctctg
    28081 gatgatcgag ccacggctgt tctcgcaagg cggcacagtc ggccgcaacc cacggcttga
    28141 cgattagtat aagccaagcg acggcgcgcg cctctgctgt gcggtcgcgg cccgattgcc
    28201 aatggcccag gcaccgttca caatacccgg atacgacatg gccctggatt ggccctggcg
    28261 tgcgaccggg catgggcatc ccaagaccca ggcacaggac cgaccgaaat gaataccata
    28321 tcaagacgca cattgctgaa gacctcggcc gctttggccg ccgcatcggc ggcttcgcag
    28381 accctcgccg ccggcgccaa cgagcggatt cgtgtgggcg tcatcggcgc ccgaaaccgg
    28441 ggctggcaga acgccgagac tttccagaaa agcgggcgat tcgagatcgc aaccttgtgc
    28501 gactgcgaca gcacttgcct ggatctggcc atggccaaga tcgagaagat cctgccggtc
    28561 aggcccgctt tggaaaagga cttccgcagg atactggatg acaagcggat cgacgccgtg
    28621 attgtcgcaa cgccggatca ctggcatgcg ctgatgacgg tgatggccct tgaagcgggc
    28681 aaacatgtgt atgtcgaaaa acccgcttcg ttcaacatcg acgacggcaa agccatggtc
    28741 gccgcccaga aacgtcaccc caagctggtc gtggccatgg gcacgcaaca gcgaagcggt
    28801 cagcacttca aggacgccaa ggccttcatc gacgatggag gattaggcac gattgccttg
    28861 gcccgcgcct ggatggccgg caatcgagtc gtggtgcaga aggttcccga cggcgacccg
    28921 cccaaagagc tggactacga catgtaggtc ggccccgggc cgatgaagcc ctacaacaag
    28981 gagaaggtac actacaactg gcacttcatg cgtgactacg gcaccaacga cgccggcaac
    29041 tggggggggc attatctcga catcgttcgt tggttcgccg atctcgatct gcccaggggt
    29101 gtctctggtt tcggcggcaa atacgtcgtc cacgacgaga aggagtggtt cgacacccag
    29161 acggccctgt ttcattatcc ccacctgacc gtcatctggg agatgagtca ctggaacgcc
    29221 gtcggtccct tcggcatggg cacgggcgcc gagattcgcg gggataaggg gacggttttc
    29281 atcgaccgtg gcgggtggac cttcctgccc gagaagggcg aaccggtcaa gcacccgtcc
    29341 agcgacctgg aaggacccca cgtgcagaac ttcgccgact gtatcacggc cggagccagg
    29401 ccggcggcag acatcatcga aggccacaaa accgccgtct tgtgccacct agccaatatc
    29461 gcgacactgc tgaaccgaac cgtctggttc gacccgaagt cgcagaccat cgagggcgac
    29521 gccgaagccg ccgctttgca gggccgcgag tatcgcaagg aatggccaat gccggtgtag
    29581 caacataccg attcggaggg acgctgaccc gcgtcgttat cattcgtcaa acctcattct
    29641 caggagaacc atgatgagct ccaatctgtc acgtcgtgct tttctgaaaa cctccgcctt
    29701 gggcgcctcg gtcgttgctc tgggcgcgcc gggagtcgcc cgtgggttcc cggccaacga
    29761 gaaggtcagg ctcggttggg tcggcatcgg cggacggtgg ggaaacctgg cggaactgat
    29821 gctccgtcac gtgcccgatg cggcgaatgt cgccgtctgc gacctgatgc cggaccgggt
    29881 cgagcggggc aaaaaagtct tcgaacgcga caagcccaac ggctacaccg atctgcgcat
    29941 gatgatcgaa aaggaaaagc ttgacggcat cctggtcgca accgagccgt gcaatcactc
    30001 ggtggtggtc gtgcgggtgt tggaggccgg catccactgt ttcgccgaga aaccgatgga
    30061 taccaccgtc gagaaagtcg acgccatcac caaggctgcg cgcaaggcca aggtgcagac
    30121 gcaga
//
LOCUS       DAOTPW010000030        30021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1618_length_30021_cov_4.936862, whole genome shotgun sequence.
ACCESSION   DAOTPW010000030 DAOTPW010000000
VERSION     DAOTPW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 30021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30021
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_30021_cov_4.936862"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="P5316_04360"
     CDS             complement(<1..195)
                     /locus_tag="P5316_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84221.1"
                     /translation="MMRISDLLPLVSLAILPSLAFAQTPAGQIVVDPAHPAWFKYHEG
                     GPFYMCGPGDPEGFLYRGTRN"
     gene            complement(179..1780)
                     /locus_tag="P5316_04365"
     CDS             complement(179..1780)
                     /locus_tag="P5316_04365"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRR84222.1"
                     /translation="MCQFRLTVGATSLVAGVLCQSCILATAFGQVFPGATWEFKTPAE
                     LGLDSAKLDQIAGIIGGSGCIVRHGYMVKTWGTQSLKGDWASASKPVISTLLFFAIEE
                     GRITSVDALVKDYGWSLSTKDQPMTFRHMANMVGNYTRAEAPGTAWAYNDYNIQLYVK
                     TLFDRVYGQSANTVALAPNRLGALQFQDGSLIASNNRLNTSVRDFARIGWFWANKGNW
                     NGSQLLPQHYFDDYMKPQVSSSLPKTAVVDPAGDYLGIGSYGGGSDQTPYGPGIYGFN
                     WWFNATGSLHPTSITWPDAPADTFQANGHWSREVVTVMPGLGIVMAYFNGATLGFEPG
                     NPGSSSNQVLKLLAESVMPTAPLIVLSKSQISRSADLGGTLSDDMFTVSNGGAGTLKY
                     AVTVDRDWLRVAPSDGTSTGEADTITVSFTVDHLAIGSHTATITVADDGSLPPASNPS
                     QTVAVTVNIRTVLPDLDLDGDVDQVDFGLFQTCLTGEEPASSPCLAADFDHNEMVNQS
                     DLTVLLGCLSGAGALADVACDDAHQ"
     gene            complement(2007..5171)
                     /locus_tag="P5316_04370"
     CDS             complement(2007..5171)
                     /locus_tag="P5316_04370"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84223.1"
                     /translation="MFPRQSAMLWAVAITFVAAGSVRATEWVADFTSVPPESWSGSDN
                     SAVANYTDTTGANPNSYTVTYQGAGNVPAGWLTQGSRAGAPTTARFWNVQDSINGRAR
                     FSTPLPTTMDLAEGISVGWKMRVGSANVTRGPIQIAATVSGSAGGSSPQAFNAYIRLR
                     NSGDPSGNSYIDIVRNGGGLYRDLTGSNDPLRVDTLVLGSNLADTYHDWSAAVVYNAS
                     DNKAYWKLWLDGTLLLFTGPSGSPTLNGEQYSFRTFQEGFTGAEYIGLGELNSQDVWD
                     FEFDCVAYKDDGLAMLTCGTTPTCESTVTPAVIQTSTALRGSAADPPSHAYTIANSGT
                     TPLNYTAVEMKKVAPPIADLYNTGVDNLRAVLGEQVLDPHWTIIAGGLDGTCTEGAPC
                     SAYTVAADGYPIPPWLANDSTSLWITASQTDDDAAAPPGQHVFRTTFTLADQAAVDAA
                     LIRGRMAYDDILADVKLNGVSVVEGTGSWGFESWQEFIIDSGFQIGTNTLDFTIQNGG
                     TTSNPMGLRVEFFDIVAGDVPWLSLDKSGGGPLAAGSGDTVTASIVNTDLTAGTHTAY
                     VAIANDCPSPTTELRRIDLTVIDCRSAVSPATNTGRAFLVGSAQTPAPVIYTFENTGA
                     PSVSYTVTSSAGWLVLDKTGGGPILPGGSDTVTGTIDVTGLEPGGYVATLTFTDACNP
                     AVQHVRQVLLSVDETIAKSGDTLQQFKAEFIDFTASDLASQSPLESCDSGGTTDRVFS
                     IGYNDVGSLPAGWLSQGSIDEVPTTAKFNNPADGLGGRVRFRTFLPFDSTFDPVKGMA
                     VAWSMRIGTDNNTRGPIQIAFPRVTGPFGTNDSTSSIEGDVFNVYVRVNDVTVRILNN
                     SGNAIGTIDQAALPNIAGQYHQWTAAVCYNAADQRAYWNLWLDGEKLMFGGADGTVVG
                     PSGDIFSFRTGLNDVSGDPYIGLGELNSGEDVWDFEFDWVRMLSYGVSGCPFWDGGGS
                     CAAPLVWPDADEDGDVDQVDFAAFQLCFSGDGNPLSDPATCRRYNRDSDNDVDTLDFS
                     KFEKCATGPGVALDPENLPAGCEL"
     gene            complement(5507..6145)
                     /locus_tag="P5316_04375"
     CDS             complement(5507..6145)
                     /locus_tag="P5316_04375"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRR84224.1"
                     /translation="MRKVSTGLCVAMFVTCAVPAVGAIILPEDDVTYRSSTRYNNTNV
                     FGLFTKGGGTTDRAYVEFLLDVTPVTSATLKLYNYWGAPQGKTVNCDVRIRAISENEA
                     GYVQWTDTAGPAPSGTSHESWTIVADLFNVNSTPAWYTFDITSLYNANLGKRVTFSIR
                     AVTTYGTADGPIFEDKENTGLSGNPPQIDWVPEPAGLMLLAVGSLLLVRRRR"
     gene            complement(6416..7276)
                     /locus_tag="P5316_04380"
     CDS             complement(6416..7276)
                     /locus_tag="P5316_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR84225.1"
                     /translation="MNRRSNGFTLIEVLVVVAIIALLVAILLPSLSRARAQARISACQ
                     SNLHQAGVAVTTYAVEYAEFIPRGGNVQRYFSNGDIHWSIVLLKQVGEKVAPLFQQAQ
                     AAGRSMQTFPDGRSYEARGIALNYLLWDKLKRVEVFHCPERAARSQDAEVLSYVVNAF
                     NPKAVTGSQGFSDTRDATRLSLWKRPAQVVYLADMEDSAVSEAAKEAYGFRDLSRFDA
                     FEPSHLPSGPGRDRRVARAMHLNRRTSCLFVDGHVEGLDSLPRGGEPDIDVSGSYSLR
                     WQRAFGVERP"
     gene            complement(7443..8672)
                     /locus_tag="P5316_04385"
     CDS             complement(7443..8672)
                     /locus_tag="P5316_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HRR84226.1"
                     /translation="MSYTIRFEADTFVPFICGYAQSRTMRRVALLIETSREFGRGLLR
                     GVARYNQEHGQWLTYCRPHGLGDMSYSWLRGWQGDGILARITDRRMAAAIAKLKVPIV
                     NLRGRLAGLPFPYVGVDNEAIARIASEHLLSRGFRHFGFCGYRRRYSADGDRRCDAFR
                     ACIESAGFRADVCAVSEPSYGQRELGSWKQELALIARWIRGLPKPVAVMACTDDRGLQ
                     VLEACRMIGVAVPDEVAVLGVANDECLCALSIPPMSSVDVNAEQIGYRAAALLDELLD
                     GRTPPEKVLIEPRSVICRRSTDVLAIEDKHVAAAIQYIREHISEQIYIGDITRHAHIS
                     RSALERRFKQALGRTVGQEIQRTRIARAQQMLADNKLPIKQVADRAGFKTVQYMTRVF
                     RTMLRQTPGAYRRAVQS"
     gene            8870..10174
                     /locus_tag="P5316_04390"
     CDS             8870..10174
                     /locus_tag="P5316_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014651565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit
                     family protein"
                     /protein_id="HRR84227.1"
                     /translation="MEKQPSTDEMAGRRQEQVIARYAIETPLSLEAAAAAMAGASTAT
                     FVDVPGLTDQLRQRVATRIERIVPRDVVSAPGLPGSRSPATGQQAARYQRGEVVLSFP
                     IEGMGTNLAALISMIMGNFFALRQVSGIRLVDLELPDCFARAYPGPQFGIEGTRRLTG
                     VHGRPIIGTIIKPNLGLSPEQTAEKVRQVAEAGVDFIKDDEVMVNPPHSPIRKRVAAV
                     MRVINEFADRTGKKVMYAFNISDEVEAMLRHHDAVVAAGGTCVMVSVNSVGYMGVLAL
                     RRRCQLPIHAHRNGWDMLTRCPHLGIEFAAWQKLWRLIGVDHLHVNGLGNKFWEPDDS
                     VVASIHAVMTPLFGGHHIMPVIGSGQWAGQAPRTFSRVRTQDLIYLAGGGIFGHPAGA
                     ASGVMSIRQAWEAAQNDVALETCATTHVELRQALEKFGSLRE"
     gene            10183..11577
                     /locus_tag="P5316_04395"
     CDS             10183..11577
                     /locus_tag="P5316_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRR84228.1"
                     /translation="MAAARPNDLLLGFYGDDFTGSTDAMEGLTRAGLRTVLFIGRPRI
                     EQLCQYQGLRAIGLAGCSRSLPPREMEVELGEAFGALKQLNLPIMHYKICSTFDSSPQ
                     IGSIGRALDVGARIFDAPVVPLLVGAPILGRYSVFGNLFARSGLDSEPYRLDRHPTMS
                     RHPITPMTESDLRLHLARQTHRKIGLLDILSLADAEQARAKYRELVAGGAEVILLDIL
                     YREHEPVIGNLLWDQVREGSTLFIVGSSGVEYALTGYWKSRGLLPASPPLSAPAVEAL
                     IAISGSCSPVTDRQIGWALQQGAAEIRLETADLVDPATAEAAIEASVAEALKLHERGR
                     TVICHSARGPADPRLQETVRRFEAMGRDARHHSGRVLGEALGTILLRVLERGKIKRVV
                     IIGGDTSFFVARTLNIEALEMVAPTAPGCPLCRAYVPGSVLNGMEICFKGGQVGKDDF
                     FGTVLVPSRSNVEA"
     gene            11662..12486
                     /locus_tag="P5316_04400"
     CDS             11662..12486
                     /locus_tag="P5316_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRR84229.1"
                     /translation="MKNNEIMIVVAPVPGEKQDEKYPGAIDVVQEVIRCEAAGAAIAH
                     LHARDEKLLQTVDPTLFAGQVRRIRQSCPIIIEGSTGGAPEHTLEQRCVTFTVPQVEM
                     GSLNLGSVNMYDGVYQNRYQDICFYADKLAAMRIAPTMAVFDLSHMANYQRLVEDGRL
                     SPPYVFEFVFDVPYALPYADRYLDLLVDHLPPGAIWFCVRHHQKGAAGLRRVMELGGH
                     IRVGYEDSPFLSNGRRARNNVELVEDAVEQAAKAGREVVPAARAREIIGLRPAEQA"
     gene            12559..13230
                     /locus_tag="P5316_04405"
     CDS             12559..13230
                     /locus_tag="P5316_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRR84230.1"
                     /translation="MKPKRLALIHTVNWYQQVITVPFVDPWLKANPDVQVFNIMDDSL
                     LEESLAHKGPTPAVIKRLQFYVLAAESMGADVAMITCTTVGEASWIARRYASIPVFNI
                     DEPMAREAVRSGRRLGIVATVPTSPAATKRLLERTAAEAGVSVEIEIALNEQAFEHLQ
                     QGRVERHNELVYREMDKLAEKVDVLVLGQISLAQIKHQTRVPVLQVGHSGFAEARRLL
                     DAAAR"
     gene            13360..14613
                     /locus_tag="P5316_04410"
     CDS             13360..14613
                     /locus_tag="P5316_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR84231.1"
                     /translation="MPKRYWLVFGMFLLSMLLYVDRVCISSAKSAIALDLGLSEKQMG
                     WIISAFALGYALFQVPSGVLADRFGPRVILSAVVTFWSLFTALTAAAFNFISALVFRF
                     LFGAGEAGAFPGCARAVYSWIPMSERGLVQGLTFSGSRFGAAFALPVIAWLVETIGWR
                     PSFVVLGVIGVGWACFWYLWFRDDPETHPRITQEEKGLILRTRQQAAAGPGPVAPLSA
                     GTLLGSSNVWLLMGQYFCSNFTFFFCLSWLFPYLRSQYTLGMVETGLYAAMPPLAGAL
                     GNWVAGLLVDRLYRRGRWIPSRRIPAILGFLLSAAGLIASVFMHTPLGAIGCLSVAVF
                     GADMTLAPSWSTCIDIGRRHSGAVSGTMNMAGNLGSFVTSLAFPYLLAWTGTHITFFL
                     VGAVLNGLAVIGWHFTRPDRPLEDH"
     gene            15456..16193
                     /locus_tag="P5316_04415"
     CDS             15456..16193
                     /locus_tag="P5316_04415"
                     /inference="COORDINATES: protein motif:HMM:NF021567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HRR84232.1"
                     /translation="MCMSEKRPLTKAGGQERIRNPAPTADPTTRTLVVYQTRPEEVEK
                     GDVWNPEYGLLWVPKDWEYLAPGDAFVTRFVKRGPHWALRGRFNRKKGYRRKLGIYAP
                     SATIREAQAAAERTEAARKEQRAKAAVVREKAEDRYRREFEQACIEFLNFSKRYAKLA
                     ARIARETTDRACQKYSGRVGRTQKLDLATKAALAVRACIRHKYTGYEDKVPPFPDPLL
                     EDEYRTARQMAHEDVDAFLERHRDKGG"
     gene            complement(16650..17006)
                     /locus_tag="P5316_04420"
     CDS             complement(16650..17006)
                     /locus_tag="P5316_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRR84233.1"
                     /translation="MAESNQVAVATPPAKPVEKPAKPIPRRQPPYHVVLLDDDDHTYQ
                     YVIEMLGKLFGHNKNKAYMMAREVDTTGRVIVDTTTKERAELKRDQIHAYGPDWRLKR
                     SKGSMRATIEPAPDAG"
     gene            complement(17129..18772)
                     /locus_tag="P5316_04425"
     CDS             complement(17129..18772)
                     /locus_tag="P5316_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR84234.1"
                     /translation="MSSAALRVLFVDDDLLDRMAFERFVKKQRLPYDYTLADSSQAAR
                     RHLAAEQFDIVITDFSLTDGNCLELLGEFRNVPFIVVTGTGSEETAVEAMKLGARDYL
                     IKDPDGHHLTVLPATVRNVLERSAAEAELQRHREHLEELVRERTAELVAEIRERLRAE
                     EEKRVLQEQLYHAQKMEAVGQLASGIAHDFNNLLTVIFSNVDLIRQQLPQDQQESIGA
                     SLEAIWEAAQQAMGVTRSLLIFARKVPGEKKPIDLRETVERTVRMIRRALPASIELVA
                     ETAGQTPIWICADETQMQQVIMNLAVNARDAMPRGGTLRIAVGPVGGPGDGGTDESKE
                     QEPALVCLTVSDTGVGMPPDIQTRIFEPFFSTKPRGAGTGLGLSIIDGIVKDHGGRVE
                     VQSEVGKGTVFRVILPTCEPGVEPEVKGPRGGRPPGRGEKIVLAEDDEYVGTVLTTRL
                     EADGYSVIRTKDGRSMMEACRQHGETIRLLVVDVDLPKRSGIDCLKDLRAEGRHTPAI
                     LMTGSTEVDLTEGLPSDTRLLRKPFQIVELTCMISEALT"
     gene            complement(18787..19197)
                     /locus_tag="P5316_04430"
     CDS             complement(18787..19197)
                     /locus_tag="P5316_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR84235.1"
                     /translation="MRPNTPILLVEDDEVDQITVQRALRDIHVTNRLLIAANGEEALR
                     MLRDPACERPGIILLDLNMPRMNGAEFLRVVKCDAELKRIPVVVLTSSREEQDRIESF
                     NLSVAGYMIKPVDYRQFVEVVRTIDLYWTLSELP"
     gene            complement(19216..21666)
                     /locus_tag="P5316_04435"
     CDS             complement(19216..21666)
                     /locus_tag="P5316_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRR84236.1"
                     /translation="MFRRQCFIYLQLLLGWATLVCFGGIGQFALAHAGPPRSRPLDDL
                     GDGRPSQLLQSLTVVWIIPENPSRAGRQEQVGREEDSARPGPAAIVSADLRAPLPAWL
                     ERAGDCEPVPKPVWERLPVGRLLRSRDELYVGQTELHGLDDRLFWWVLLVVALAIVLA
                     ICWNRTPCRDSGAGTVAPHGGLGAERLDDETTRETARRFRAIFDQSLHLTGVMDPQGT
                     LLDVNSPAAEFSGVSREELLHKPFWEGPWWRHDSAARSQVREAIARVAHDGEPVRFQT
                     THVDRDGRLHNIQFSLRAVRDDIGRIALLIAEGLDITDLKRSEQAAQDGREWYRSLFE
                     GANDAIFLMEEDRFVDCNPRTQALFGGTREQILQLHPYDLSPEFQPDGRPSTQKALEK
                     ISAAYAGEPQSFEWRHYRIDGSLFDAAVTLNRVFLAGRPHLVAVVRDITGRKRADEAL
                     RNEMAFSQTLIHASPAFFVAIGADGRTRMMNPAMLNALGYTAEEVVGKDYMTTFVPDA
                     DRGALARIFEQLVVAGRPTLNENHIMTKDGRLLLVEWHGAPVFKGQELDYFFGVGIDI
                     TRRRQAEEEIRKLNAELEQRVRDRTAQLAAANAALNEFAYVVSHDLKAPLRAVSQLAQ
                     WVSEDYASVLDEEGKNRLKLINGRIARMYNLIDGILQYSRIGRVEEDRRPIDLDLLVR
                     EVIEALSPPAHVHVTVEGRLPVVVADRTQMTQVFQNLIGNAIKFINKPAGLVTVACED
                     AGDRWRFSVTDNGPGIDPKYHEKIFGIFQTLTPRDQQEGTGIGLTLVRRIVELYSGKV
                     ELESEVGKGSTFTFTLPK"
     gene            22027..22869
                     /locus_tag="P5316_04440"
     CDS             22027..22869
                     /locus_tag="P5316_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84237.1"
                     /translation="MAGPTHTPKPAEERPPARIHILLAQEVPVGVVFRRGPSKQVCVI
                     GWNRANDTFMVGQWLKGRIYERRSDLSPDGRYLIYFALKGKPGTDKSVSYTALSKAPF
                     LKPIGLWEKADSQHGGGLFLNRTTYWINDERSHKTVTVPRELRKVPHYRGEYSFGSEC
                     AGVYFLRLQRDGWTLKEAPSGPEGRSFAVFEKPAGAGWVLRKTAHVTPLNSPDAHELV
                     HTTAGTRIDLPGWEWADIDRNRVVWSTAGKLFAAQISETGTFEPKQLFDFNPLRFVVR
                     TAPY"
     gene            complement(22989..23549)
                     /locus_tag="P5316_04445"
     CDS             complement(22989..23549)
                     /locus_tag="P5316_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84238.1"
                     /translation="MRNVSVILILGVLGCEGPLTVPAVERLEPEGQKTVDESWLNMLS
                     PPERLDRSLLLDTVLTNHFHQRGVDRLNLTSEKDVWGGRVIMTVYYDRAHPAFDSFTV
                     AYIDAQGRERRRERYTFEEVKGRVAELVGTASEPGAQPAATPEEIERQRKAEEEMANR
                     LARQILIRAATRPAAQPVDESEVVPP"
     gene            complement(23741..25075)
                     /locus_tag="P5316_04450"
     CDS             complement(23741..25075)
                     /locus_tag="P5316_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRR84239.1"
                     /translation="MIRSIRTRRRAWPRSPRRPRPAFWLSAATVALVMMLTAAAAKAA
                     QSEVARPAPPPDVKAVADANNRFALDLYGKLKNGDENLFFSPYSISTALAMTYAGART
                     STAEEMAKTLHFDLPAERLHSAYAEMIRDLNEAGLGTAEPGEAGKKGDFQLSVANRLW
                     GQKGEQFLKEFLDLTERSYGAGLSEVDYVGDTEGARKTINDWVEKQTADKIKELIKQG
                     VLNAATRLVLTNAIYMKAEWLAKFKKQDTNPAPFFAAGGKEIQVPTMHQTSILGYAEM
                     DNLQALELTYKGNRLSMIILLPEQKNGLAGLEKRLTPNNLSQWLAQLKPQKVRVALPK
                     FKATREFSLAQVLQSLGMTKAFDGNQADFSGMNGKKNLFISAVVHKAFVNVDEEGTEA
                     AAATAVVMKLTAAPMPERIPEFRADHPFVFLIRDRVSGAILFMGRVAEPKTE"
     gene            complement(25411..27123)
                     /gene="metG"
                     /locus_tag="P5316_04455"
     CDS             complement(25411..27123)
                     /gene="metG"
                     /locus_tag="P5316_04455"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRR84240.1"
                     /translation="MSKRRFLITSALPYSNGRLHVGHIAGAYLPADTYVRYLRARGDE
                     VRFICGSDDNGVASLISARKEGKSVEELTAFYNARQAADFAGLGIRFDIYGGTHQPKF
                     VDLHNRISQDFFRVIHEKGYFVKKTTMQLYDVQADQFLPDRYVKGKCYNCGADGAYGD
                     QCEACGVSIDPMKLINPVSTITNTKPEPRETTHWYMQLPQFEERLRAWLEAKRQPQGD
                     CPPWRETVLNFSLGQIKQGLPERAMTRDLHWGVPVPLNDPDAAGKVLYVWFDAPIGYV
                     SFTAELCRQVDGDWKAYERWWKDPDCRIVHFIGEDNTVFHALTWPAMLMAEGSFQLPW
                     QVVANSFLNIKFPGKEEEKISKSRGTAIWIEEYLKMFEPDPLRYYLTAIAPETQRTTF
                     DVDDFISRNNGELVNALGNFVNRTMTFAQKYFDNKVPAVGNRQQVDNDHLANIAAHAE
                     RITARLDGLRFRAAIEELMNLARASNVYFDAKKPWQQRKDDLAACGTSLNVCIQTVKA
                     LMTLMAPFLPFSADKCLRMLNLDAGALSWDQTTTELPPGHPLGEPAILFQKLDPKVVF
                     ADMQ"
     gene            27528..28250
                     /locus_tag="P5316_04460"
     CDS             27528..28250
                     /locus_tag="P5316_04460"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRR84241.1"
                     /translation="MAKLGVNIDHVATVRQARRTIEPDPVWAAAEAQLGGADGITFHL
                     REDRRHINDRDAALLKQTVTCKLNMEMSIAPAIVRIAENLRPNQCTLVPERRQELTTE
                     GGLDVIRFKRRIAPVVQRLHRKGIEVSAFIEPIAEQVKMCADLDFDAIELWTGGYAHA
                     RGARARKRSLKQLETALELGWERGLVVHGGHGLTYDNIGPVAEMEFEEFNIGHSIVAR
                     AILVGMREAVRQMKVLATGVFQ"
     gene            28289..29914
                     /locus_tag="P5316_04465"
     CDS             28289..29914
                     /locus_tag="P5316_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRR84242.1"
                     /translation="MSTAAVLLATIATTLSAQPTSSASAPVTATASVPAPVETAAPAP
                     PPVRATLPAPSPATNAAPAPPTAPASMPTTAPAPPGSLAIREGDVIVFLGDELSETPD
                     ARRGGRPTFPQLVETFLTVRYPELHRSPSADGPRPVRYISVGWAGDTAARALLRLERD
                     VLIHKPTVVVVCLGINDAGYLSFVPARLEAMQRDLTKIVQKCHEAGARVWLMSPPCVE
                     EERGKKARVVRDGQRAVADLEVIRYNETLAKYAAAMREIAAANPPAGFADWFAEASAA
                     SRQVRAYPGEDWLTTDGRTLTFHGTTIGATTLLRAWGAEPIRALLELDWTDGTARIST
                     HQGQSSTMLVQIADDEKRILSVDNLPLPWPLPSEAGVGLQSGWKAADLCQIILRVPDP
                     PEQGITLVQETDDHGATAQWPITAAQLQAGFNLVTAEPLRSPKGVRDLYNLIVTKNRT
                     RDTIWRRLELSAPQEPELTDGHRQLIEAWKAYVAGYERIIYRYPKTFSARFTLSETTE
                     SEHLPTSQPTRLPRVVPSSHNPIPATLPAAVHP"
BASE COUNT         5586 a         9107 c         9378 g         5950 t
ORIGIN      
        1 attgcgcgtg ccccggtaca agaagccctc cggatctccg ggaccacaca tgtagaatgg
       61 cccgccctcg tggtacttga accaggcggg atgagcgggg tcgacgacga tctggccggc
      121 gggcgtctgc gcgaacgcca aggacgggag aatcgccagc gacacgagcg gcaacaaatc
      181 actgatgcgc atcatcgcag gccacatccg caagagcgcc ggcgcccgat agacagccca
      241 ggaggaccgt caggtcggac tgatttacca tttcgttgtg atcgaaatct gcggccagac
      301 agggggacga agccggctcc tcccctgtca ggcacgtctg gaacaaccca aagtctacct
      361 gatctacatc gccgtccagg tcgaggtctg gaagcaccgt cctgatgttg accgtgacag
      421 ccacggtttg agacggattg gaagccgggg gcaggctgcc gtcatcggcg acggtgatcg
      481 ttgcggtgtg tgacccgatt gccagatggt ctactgtaaa cgaaaccgtg atggtgtcgg
      541 cttcgccggt ggacgtgcca tcggacggag cgacacgcag ccagtcgcga tcaacggtaa
      601 cggcgtattt caacgtccct gctccgccgt tggacacggt gaacatgtcg tccgacagag
      661 tcccgccgag gtccgccgat cgactgattt gactcttgct tagcacgatc agcggagccg
      721 tcggcatcac cgattcggcc agcagcttaa gcacctgatt cgagctggag ccagggttgc
      781 ccggctcgaa gccgagtgtg gctccgttga agtacgccat cactataccg agaccgggca
      841 tcaccgtcac cacttcccgg ctccaatgcc cgtttgcctg aaaggtgtcg gcgggagcat
      901 cgggccaggt gattgatgtc gggtgcagac tccctgtggc gttaaaccac cagttgaagc
      961 cgtagatgcc cgggccgtag ggggtctggt ccgatccgcc gccataactt ccgatgccca
     1021 gatagtctcc ggcgggatcg actacggcgg tcttgggcag gcttgaggaa acctgcggct
     1081 tcatgtagtc gtcgaaatag tgctgaggca gcaactggct gccgttccaa ttgcccttgt
     1141 tggcccagaa ccacccgatg cgggcaaaat cgcgaaccga ggtgttcagc cggttgttgg
     1201 atgcgatcaa cgaaccatcc tgaaactgca gcgcaccaag gcgattaggg gccaaggcca
     1261 ctgtgttggc gctctggccg tagacacgat cgaacaacgt cttgacatat agttggatat
     1321 tgtagtcgtt gtatgcccaa gcggtgccgg gggcctccgc ccgcgtgtaa ttcccgacca
     1381 tgttggccat gtggcggaag gtcattggct ggtccttggt gctgagcgac cagccgtagt
     1441 cctttaccag ggcgtcgacg ctggtgatac ggccttcttc gatagcgaag aacagcagcg
     1501 tgctgatcac cggtttcgag gccgatgccc agtcgccctt gaggctttgc gtgccccagg
     1561 ttttgaccat gtacccgtgg cggacgatgc atccgctgcc cccgatgatg ccggcaatct
     1621 ggtcaagttt ggctgagtca agtccgagtt ccgcaggggt tttgaactcc cacgtcgccc
     1681 caggaaacac ctggccgaag gcggttgcca ggatgcacga ttggcaaaga acaccggcca
     1741 ccaagctggt ggctccgaca gtgagccgaa actggcacat cgaccgtact tctcctccgc
     1801 gaacgcttct gtcaggtgat ccccaacacg ccaaggcgta gagtttggat ctcgccaatc
     1861 gcatggcggg cagaatgacc gtctccacgc cgcttccggg cacaatccat tgggctgcgg
     1921 gtccggtggc cggcggcgcg actgcctgcc gtccccttct tcgtgggcaa agacagacgc
     1981 cattggctgg gccagccccc aggccgctat agttcgcaac cggcgggcag attctccgga
     2041 tccaacgcga ctcccggacc ggtcgcgcat ttctcgaact tgctgaagtc cagtgtatcc
     2101 acgtcgttgt ctgagtcccg attgtaacgc ctgcacgtcg cgggatctga cagcgggttg
     2161 ccatcgcccg agaaacacaa ctggaaggct gcgaagtcca cctggtcaac gtcgccgtcc
     2221 tcgtcggcgt ccggccagac cagaggagcg gcacaactgc ctcccccgtc ccagaaagga
     2281 cagccgctca cgccatagga gagcatccga acccaatcga actcgaaatc ccagacgtct
     2341 tcgccggaat taagctcgcc caggccgatg tacgggtcgc cgctcacatc attcaggccg
     2401 gttcggaagc tgaatatgtc gccgctcggc ccaacgacgg taccatctgc gccgccgaac
     2461 atcagctttt cgccatccag ccacaggttc cagtacgccc tctgatcggc agcgttgtag
     2521 caaaccgcag cggtccattg gtgatactgg ccggcgatgt tggggagggc cgcctgatcg
     2581 atggtgccaa tggcgttgcc gctgttgtta aggattctga cggtgacgtc gttcacgcga
     2641 acatagacat taaacacatc gccctcgatg gaagaagtcg agtcattggt gccgaacggg
     2701 cctgtgacgc gcgggaaggc gatctggatc ggcccacggg tgttgttatc cgtcccgatt
     2761 cgcatactcc aggccacggc catgcctttg accggatcga aagtgctgtc gaaaggcagg
     2821 aacgttcgga accgcacccg gccgccgaga ccgtcggccg gattgttgaa cttggccgtg
     2881 gtgggtacct cgtcgatgga tccctgggag agccaacccg ccggcaatga acccacgtcg
     2941 ttgtagccga tcgaaaaaac gcggtcggtt gtcccgccgg agtcgcacga ttcgagcggg
     3001 ctttgggagg cgaggtccga tgcggtgaaa tcgatgaact ccgctttgaa ctgctgcaag
     3061 gtatcacctg acttggctat tgtctcgtcg accgagagca gcacctgtcg gacgtgctgc
     3121 acggcggggt tacaggcgtc ggtgaacgtc aaggtggcga cgtagccgcc cggctccagg
     3181 ccggtgacgt cgattgttcc tgtaaccgtg tcagagccac ccggaaggat cggtccgccg
     3241 ccggtcttat cgaggacgag ccagcccgcg gaggatgtga cggtgtagct cacactggga
     3301 gcaccggtgt tttcgaaagt gtagatcact ggagcgggag tctgggcaga ccctaccagg
     3361 aacgcgcgac ccgtgttggt tgccggggac accgccgacc gacagtcaat gacggtcaga
     3421 tcgatccggc ggagttccgt ggtgggcgat gggcaatcgt tagcaatagc cacgtatgcc
     3481 gtgtgggttc cggcggtcag atcagtattg acaatcgaag cggtgacggt gtcgccggat
     3541 cctgcggcaa gcggcccgcc gccgctcttg tcgagactca gccaaggtac gtccccggct
     3601 acaatgtcaa agaactcgac gcgcagaccc atcgggttgg aggtggtgcc gccgttctgg
     3661 atcgtgaaat cgagggtatt tgttccgatc tgaaatccgc tgtcgatgat gaactcctgc
     3721 caggattcga agccccacga gccggtaccc tccaccacgg atacgccgtt gagcttgacg
     3781 tcggccagaa tgtcgtcgta cgccatccgg ccccggatca gggccgcatc caccgccgcc
     3841 tgatcggcca gcgtgaaggt ggtccggaaa acatgttgtc cgggaggggc agccgcatcg
     3901 tcgtcggtct gactggccgt gatccacagc gaggtcgagt cattcgccaa ccacggagga
     3961 atgggatacc cgtctgcggc cacggtgtat gctgagcagg gagcgccttc cgtacaggtc
     4021 ccgtccaggc cgcccgcaat gatcgtccag tgcggatcga gcacctgttc gcccagaacc
     4081 gccctcaaat tgtccacgcc ggtgttgtag aggtcggcaa ttgggggggc gaccttcttc
     4141 atctccactg cagtgtagtt cagcggggtg gtgccgctgt tggcgatcgt ataggcgtgg
     4201 ctcggcggat cggcggcact gccccgcagc gccgtcgatg tctggattac cgccggtgtg
     4261 acggtgcttt cacaggtggg cgtagtccca caggtcagca tcgcaaggcc gtcgtccttg
     4321 taggccacgc agtcgaactc gaaatcccag acatcctggc tgttgagttc gcccaacccg
     4381 atgtactccg cgccggtgaa accttcctgg aatgtccgaa aactgtactg ttccccgttc
     4441 agcgtcggcg agccggaggg accggtgaac agcagcaggg ttccgtcaag ccaaagcttc
     4501 cagtaggctt tgttgtcgct ggcgttatac accaccgccg cggaccagtc gtggtacgta
     4561 tccgccaggt tactgcccag taccagcgta tccactctca gcgggtcgtt gctgccggtc
     4621 aagtccctat acagtccgcc gccgttacgc acgatgtcga tgtagctgtt gccgctcggg
     4681 tctccggagt tccgcaggcg gatataggca ttgaacgctt gggggctgct gcctccggcc
     4741 gatccgctca ccgtggccgc gatttggatc ggtccccggg ttacattggc cgatccgacc
     4801 cgcattttcc aaccgacgga gatgccttca gcaaggtcca tggtggtcgg cagaggggtt
     4861 gagaagcggg cccgtccatt tatcgaatcc tgtacattcc agaacctggc ggtggtcggc
     4921 gcgccagctc ggcttccttg agtcagccac cccgccggca cgttgcccgc gccttgatac
     4981 gtgactgtat acgagtttgg gttggccccc gtggtgtcgg tatagttcgc cacggcgctg
     5041 ttgtcggacc ctgaccagct ttctgggggc actgacgtga aatcggccac ccattcggtt
     5101 gcccggaccg atcctgctgc cacaaaggtg atggccaccg cccacaacat agctgattgt
     5161 ctcggaaaca tagctatctt cctttcctgc gtctcgccaa gatcgcctgg aattgactcc
     5221 tccgcgagtc gtcttcgtca cctcaacccc tcaccccgag cgaggtccat cgccggtcga
     5281 cccctttgct gcgatacgac ctgggggttt gggcgtactg gacgccaggc cgcacccata
     5341 cctcagaagc ccgggcccag atgcacaaac cgtccctcct ggcaggctag cagcccactg
     5401 caacttcctc catcattgtc cctctctccg gcggttgcgc ttcgggcata tctccgatgc
     5461 acaatcaccc tcgcgccgcc ttccagcgtc ttaaaagacg accccctcac cggcggcggc
     5521 gtaccagcaa caacgagccc accgcgagca gcatcagacc ggccggctct gggacccagt
     5581 cgatctgagg agggtttccg gacaagccag tgttttcctt gtcctcaaat atggggccgt
     5641 ctgcagttcc gtaggtggta acggcgcgga tgctgaacgt gaccctctta cccagattgg
     5701 cgttgtacaa agacgtaatg tcaaaggtat accaagccgg ggtgctgttc acattgaaaa
     5761 gatccgccac aatcgtccaa ctctcgtggg atgtgcccga cggcgccgga cctgcggtat
     5821 cggtccactg gacatatcca gcttcattct cgctgatcgc gcgaatccgg acatcgcagt
     5881 tcactgtctt gccctgcggg gcaccccaat agttgtagag tttgagtgta gccgacgtta
     5941 ccggtgtcac gtccaagaga aattccacgt acgcacggtc agtggttccg ccacccttgg
     6001 taaacaaccc gaagacattc gtgttgttgt agcgagtgct gctccggtaa gtaacgtcgt
     6061 cctccggcag aatgatcgca cccacggcgg gcaccgcaca cgtgacgaac atggccacgc
     6121 acaatccagt cgatactttt ctcatcttga acctcctcct catttgatac ctaaggcgga
     6181 ccgccttttt gcgggccgca acccaaacgt tctgcgatcc agaaacatgc gcgcaaaatg
     6241 cacacccgtt gcgacggacg agactaatag aaagcaccat agctctcttg cttttcttgt
     6301 tatccgtccg gcctcggggc tgaccttatc gagatcaatg ccgacaccgg gcactccaag
     6361 agctcttgat ttcattgatc ggtcgcgaga tacaccctcc ttcctgctgc cggcgtcagg
     6421 gcctttccac tccaaaggcc cgctgccatc gcaacgagta actgccggaa acgtcaatat
     6481 cgggctcccc cccgcgcggg agggaatcca ggccttcaac gtgaccgtcg acaaacaggc
     6541 agcttgtgcg gcgattcaga tgcatcgccc tggcgacgcg ccggtcgcgg ccgggaccgc
     6601 tgggcaaatg gctcggctcg aaggcatcga accggctgag atccctgaac ccataggcct
     6661 ccttggccgc ctccgagacg gccgagtcct ccatgtccgc caggtaaacg acctgcgcag
     6721 gccttttcca gagcgagagt cgtgtcgcat ctctcgtgtc cgagaatccc tggctcccgg
     6781 tgacggcctt cggattgaaa gcgttcacca cgtaactgag aacttccgcg tcctgggatc
     6841 tggccgcgcg ctcgggacaa tgaaacacct ccacgcgctt cagtttgtcc cagagcaggt
     6901 agttgagggc tattcccctg gcttcgtagg atctgccatc cgggaacgtc tgcatgctcc
     6961 tgccggccgc ctgggcctgt tgaaacaggg gggcaacctt ctctccgacc tgtttgagca
     7021 ggacgatgga ccaatgaatg tccccgttgg aaaagtacct ttgcacgttg cctccgcggg
     7081 ggatgaactc cgcgtactct accgcgtaag tggtgaccgc cacgccggcc tggtgcagat
     7141 tgctctggca cgccgagata cgtgcttgag cccgcgcccg ggatagcgat ggaagcagaa
     7201 tcgccaccag cagcgcaata atcgccacca cgacgaggac ttcaatcaac gtgaatccat
     7261 tcgaccgtcg gttcattgat gcagctccgt cttgaaatga tggcacctgc ctctcgggac
     7321 tcttcgggcg gagtccctct tccgactgat acctatgcta tcgggcgacg atcagccggt
     7381 tcaagggcaa aaggccccgg agttttgtct tttatggaaa aaggtttgta ctttcgggtc
     7441 gctcaggact ggaccgcccg acggtaggcg cccggggtct gcctgagcat cgtccgaaac
     7501 acccgggtca tgtactggac ggtcttgaag cccgcccgat ccgcgacttg tttgatcggg
     7561 agcttattgt cagccagcat ctgttgagcc cgggcaatcc gcgtccgttg aatctcctgc
     7621 cccactgtgc gccccaatgc ctgcttgaat cgacgctcca gcgccgatcg tgaaatatgt
     7681 gcatgtcggg taatgtcccc gatgtagatc tgctcgctga tatgctctcg aatgtattga
     7741 atggccgctg caacatgctt atcttcgatc gccaacacat cggtggaacg tcggcaaatc
     7801 acagatcgag gttcgatgag caccttttcc ggcggcgttc ggccatcaag gagttcatca
     7861 aggagtgcgg cggcgcggta gccgatctgc tccgcattga cgtccacgct gctcataggc
     7921 ggaatggaga gtgcgcacag acactcgtcg ttggccactc cgagcaccgc cacttcatcg
     7981 ggcaccgcga ccccgatcat gcggcacgct tcgagtacct gcaggccgcg gtcatcggtg
     8041 caagccatga ccgccaccgg cttgggtaga cctcgaatcc atcgtgcgat cagggcgagt
     8101 tcctgcttcc aggaacccag ttcgcgctga ccgtaagagg gctccgacac ggcgcagacg
     8161 tcggccctga agcctgcgga ctcgatacag gcccggaagg catcgcatcg ccggtctccg
     8221 tcggcgctgt agcggcgtcg gtaaccgcag aaaccgaaat gccgaaaccc gcgactgagc
     8281 aggtgttcag aagcgattcg cgcgatagcc tcattgtcca ctccgacata gggaaaggga
     8341 agaccggcca accggccgcg aagattgacg atcggtactt taagcttggc gattgcggcg
     8401 gccatccgcc ggtcagtaat ccgggcaagg atgccgtcgc cctgccaccc gcggagccaa
     8461 gaataggaca tgtcgcccag accgtgcggc cggcaatacg tcaaccattg cccgtgttcc
     8521 tggttgtatc gggcgacacc gcgaaggagt ccgcggccga actcgcgaga agtctcgatg
     8581 agtaaggcaa cccgccgcat ggtccggctc tgtgcatatc cgcatatgaa aggaacgaac
     8641 gtatccgcct cgaagcgaat agtataactc atttctcaaa aaagacaaca tccgccggtc
     8701 ccacaccgta ggcgcgacgt ggctcgcccc gttaggattg agtcgtacct ctgagccgac
     8761 gcggcatttg accggcttga gggggctccg agcacggaaa gggcacgggc cgatgcggcg
     8821 cagcagacca cctcgaatca cgagagcgaa aggatctgat ctcctccagg tggagaaaca
     8881 gccgtctact gacgagatgg ccgggcgtcg gcaagaacag gtcatagccc ggtatgccat
     8941 cgagaccccc ctttctctgg aggcggcggc cgcggccatg gccggggcct cgactgccac
     9001 ctttgtcgat gtgccggggc taaccgatca gttgcggcag cgcgtggcaa caaggatcga
     9061 gagaatcgtg ccccgggacg tcgtcagcgc acccggtctt ccaggatcgc ggagtccggc
     9121 cacggggcaa caggctgcga ggtaccagcg gggagaggtc gtgttatcct ttcccatcga
     9181 gggcatggga acgaacctgg cagcactgat ttccatgatc atgggcaact ttttcgccct
     9241 cagacaggtg tccgggatac gtctggtgga cctggagttg cccgattgct tcgcaagggc
     9301 ttatccgggg ccgcaattcg ggatcgaggg aacgcgccga ctgacgggcg ttcacggccg
     9361 gcctattatc ggaacgatca tcaagcccaa tctcggcctg tcgccggagc agacggcgga
     9421 aaaggtgcgc caagttgccg aagccggcgt ggatttcatc aaggatgacg aggtgatggt
     9481 caacccgcct cattcgccta tcagaaagcg ggtggcagcg gtcatgcggg tgatcaacga
     9541 gttcgccgat cggacgggca agaaggtgat gtacgccttc aacatcagcg acgaagttga
     9601 ggcgatgctt cgtcatcacg acgcggtagt ggcagcgggc ggcacgtgtg tcatggtcag
     9661 cgtcaacagt gtcggctaca tgggggtttt ggcgctgcgt cgccgatgcc aacttcccat
     9721 tcatgctcat cggaatggtt gggacatgct gacgcggtgt ccgcacctgg gcatcgaatt
     9781 cgcggcctgg cagaaactct ggcggctgat cggcgtggac cacctgcacg ttaacggtct
     9841 gggcaacaag ttctgggagc cggatgattc ggtggtcgct tccatccatg cggtgatgac
     9901 tccgctgttc ggtggtcacc acatcatgcc ggttatcggc tcaggccaat gggccggcca
     9961 ggcgccccgc acgttctcga gggtgcgaac gcaggatctg atttacctgg ccggcggggg
    10021 catcttcggc catccggccg gagccgcatc cggggtcatg tccatccgcc aggcatggga
    10081 agccgcccaa aacgacgtag ccctggaaac atgcgccaca acccacgtgg agctgcgaca
    10141 ggctttggaa aagttcggct ctctgcgcga gtaggaacgt tcatggccgc ggccagaccc
    10201 aatgaccttc ttctgggctt ctacggagac gacttcaccg gttccaccga cgcgatggag
    10261 ggtttgacgc gtgcgggatt gcgaacggtg ctgttcattg gtcgtcctcg catcgagcaa
    10321 ctgtgccaat accaaggatt gcgggccatc gggttggccg gttgcagtcg ttccctgcct
    10381 ccgagggaaa tggaagtgga actgggcgag gcattcgggg cgctgaagca gttgaacctg
    10441 ccgatcatgc attacaagat ctgctcgacc tttgattcct cgcctcagat cgggagtatc
    10501 ggccgggcct tggatgtcgg ggcaaggatc ttcgatgctc ctgtcgtgcc gctgcttgtc
    10561 ggggccccca ttctgggacg ctatagcgtc ttcggaaacc tcttcgcccg gtctggtttg
    10621 gactccgagc cctatcggct tgaccgtcat cccacgatga gtcggcaccc gatcaccccg
    10681 atgacggaaa gcgacctccg ccttcatctg gcgcggcaga cacatcggaa gatcggactg
    10741 ctcgacatcc tgtcgctggc cgacgcggaa caggccaggg cgaaataccg cgagcttgtt
    10801 gccggaggag cggaagtgat cctcctcgac atactctata gggagcacga gccggtaatc
    10861 gggaatctcc tgtgggacca ggttcgcgag ggatccacgc tgttcatcgt cggctcctcc
    10921 ggtgttgaat acgccctgac cggatactgg aagagccggg gcctcctgcc ggcgtctcca
    10981 cccctttctg ctccggcggt cgaagcactg attgccattt cgggcagttg ctcgccggtg
    11041 acggatcggc aaatcggctg ggcattgcag caaggggctg cggagattcg cctcgagacg
    11101 gcggacctgg tggacccagc caccgccgaa gcggcgattg aagcatcggt ggccgaggcg
    11161 ctgaaactgc acgaacgcgg gcgaacggtg atctgccaca gtgcccgggg gcctgcggac
    11221 cctcgcctgc aagaaacggt aagacgcttc gaagccatgg ggcgcgatgc caggcatcac
    11281 agcggcagag ttcttgggga ggctttgggc accatcctgt tgcgggtgct ggagcgagga
    11341 aagatcaaac gcgtagtaat catcggcggc gacacatcct tcttcgtggc aaggaccctg
    11401 aacatcgagg ctcttgagat ggtggcgccg accgctcccg gttgcccgct gtgccgggcc
    11461 tatgttcccg ggtcggtgct gaatgggatg gagatttgct tcaagggcgg acaggtgggc
    11521 aaggacgact tctttggaac cgtgctggtt ccgagccggt cgaatgtcga ggcttgaccc
    11581 acgttccagc cgcgggcata aggggggcaa gcaagccggt tgtcccggag acaagggtcg
    11641 cgggcccgac ggagttcagg catgaagaac aacgaaatca tgatcgtggt cgcgccagtt
    11701 ccgggcgaga agcaggatga gaaatacccc ggcgcgatcg acgtggtcca agaggtgatt
    11761 cgttgtgagg ccgccggcgc cgcgatcgca cacttgcacg cccgcgacga aaaactgctg
    11821 caaaccgtcg atccaaccct ttttgccggt caggtgcgcc ggattcgcca atcctgtccc
    11881 atcatcatcg aaggctcgac cggaggcgcg cccgagcaca cgctggaaca acgctgcgtc
    11941 acattcaccg tcccccaggt cgagatggga tccctcaacc tcgggtcggt caacatgtac
    12001 gacggcgtgt accagaacag ataccaggac atctgtttct atgccgacaa gctggcggcg
    12061 atgcgaatcg cgccgaccat ggccgttttc gatctgtcgc acatggccaa ttaccagcgg
    12121 ctggttgagg acggtagact ctcaccgccg tacgtgtttg agttcgtctt cgacgtgccc
    12181 tacgcgctgc cctatgcgga tcggtatctc gatctgctgg tcgatcatct gccgccgggg
    12241 gcaatctggt tctgtgtgcg acatcaccag aagggagcgg ctggcctgcg aagggtaatg
    12301 gagttaggcg ggcatattcg cgtcggctat gaggacagtc cttttttgag caacgggcgc
    12361 cgggcaagga acaacgtcga gctggttgaa gacgcggtgg aacaggccgc caaggccggg
    12421 cgtgaggtcg tgcctgccgc tcgcgcccgt gagatcatcg gtcttcggcc ggcggaacag
    12481 gcctgaccgc gcgcccgccg aatgaaacgg ttgtatcggt gcactgttcc gtcttgagcc
    12541 gtttctggag gacccaccgt gaaacccaag agactggccc ttattcatac ggtgaactgg
    12601 taccagcagg tcatcaccgt tccgtttgtg gatccctggc tgaaggcaaa ccccgacgtg
    12661 caggtgttca acatcatgga tgattccctc ttagaggagt cactggcaca caaaggtccc
    12721 actcccgcgg tgatcaagcg tcttcagttc tatgttctgg ccgccgaaag catgggcgcc
    12781 gacgtggcga tgatcacctg taccacggtg ggtgaggcct catggatcgc ccgcaggtac
    12841 gcatcgattc cggtcttcaa catcgacgag cccatggctc gcgaggcggt tcgatcgggt
    12901 cgtcggctgg gtattgtcgc gacggttccc accagcccgg cggccaccaa gaggttgctc
    12961 gaacgcaccg ccgcggaagc cggcgtctcc gtggaaatcg aaatcgcctt gaacgagcag
    13021 gcattcgagc atctccagca ggggcgggtc gaacggcaca acgaactggt gtaccgggaa
    13081 atggacaagc tggccgagaa ggtggacgtg ctggttctgg ggcaaatcag cctagcccag
    13141 atcaaacatc agacgagggt gccggtgttg caggtgggac actcgggctt cgctgaagcc
    13201 cgccggctgc tggatgcggc tgctcgctga gcccaatgga gaagagcaca acgttgccct
    13261 acgatggcga ctttcagcgc cggaccgaaa cggcgttgta cgttggggca ggcgtcgctt
    13321 cataggaatg acaggcaatt gccactcgag atcctccgga tgcccaaacg ctattggctg
    13381 gttttcggga tgtttctgct gtcgatgctg ctttacgtgg accgggtgtg tatctcgtcg
    13441 gccaagagcg ccatcgccct ggacctgggc ctgtctgaaa agcagatggg ctggatcata
    13501 tcggcctttg ccctgggata cgcgctgttc caggtgcctt cgggcgtcct ggcggaccga
    13561 ttcggaccgc gggtcatcct cagcgcggtg gtgactttct ggtccctgtt cacggccctg
    13621 accgcagcgg cgttcaactt catctccgct ttagtgttcc gctttctttt tggagccggc
    13681 gaggccggag cctttccggg gtgcgcccgc gccgtctact cctggattcc gatgtcggaa
    13741 cgagggctgg ttcaaggatt gactttttcc ggctcgagat tcggggccgc ttttgcgctc
    13801 ccggtcatcg cgtggctggt tgagacgatc ggttggcggc cgagtttcgt tgttcttggc
    13861 gtcatcggcg ttggctgggc gtgtttctgg tacctctggt tccgcgacga cccggaaacc
    13921 cacccgcgca tcacgcaaga agagaagggc ctgattcttc gcacgcgcca gcaggccgcg
    13981 gcgggccccg gaccggtcgc ccccctcagc gcgggcactc tgctgggcag ctccaacgtc
    14041 tggttgctca tggggcagta tttctgctcc aacttcacat tctttttttg cctgtcttgg
    14101 ctgtttccgt accttagaag ccagtatacg ctgggcatgg tcgagaccgg cctctatgcc
    14161 gccatgccgc ccctggcggg ggctctgggc aactgggtgg ccggcctgct ggtggaccgg
    14221 ctttatcggc gaggtcggtg gataccctcg cggcgaattc ctgccatcct gggcttcctg
    14281 ctgtcggccg cgggtctgat tgccagcgta ttcatgcaca cgccgctggg cgcgatcgga
    14341 tgtctgtcgg tggcggtctt cggtgccgac atgacgcttg ccccgtcatg gtcgacctgc
    14401 attgacatcg ggcgccggca ttcgggtgcg gtctcgggga cgatgaacat ggcgggcaac
    14461 ctcggatcct ttgtcacttc gctcgcgttc ccgtatctac tggcctggac cggcacgcac
    14521 atcaccttct tcctggtcgg ggcggtgctg aacgggctgg cggtcatagg atggcatttc
    14581 acgcgtccgg atcgaccgct tgaggaccat tgagaataag atccgaatgt gtccccgggc
    14641 gagaaagaca caggacacta ccgccaaggc acgtccggca cgcgataacc aggttgccgg
    14701 catccgcgtg gccgctggta aagcccgagg gcttggtctg cctaacggtc gcgtcatggt
    14761 acgggcatcg ccggcggacg ctacgtgatc cagcgcatta tgcctggtcg ggtcctccgc
    14821 aactgaaggg catcttggcg ttgggttcgt tcaacgaagc ttttccgccg gcgaccggac
    14881 caaccccgct tgccatgggg ccatcctcgc ggcggaaaaa ctctgttcgt ttggcgcggg
    14941 gaagctatca gccgtcggca ctcagctttc ggccggacgg aattccgtgc tgcatgctga
    15001 tctctgagag ctggttgctc cctcgttggg ttcgtttggt cagaaggagt gttgatctct
    15061 gaggtatcag ctcttggctc tcggccggag gggattgatc ttcagttttc aaagagcatt
    15121 ggcgggggcg gcagacggtt gggttccggt cggcgcgcag ggtctccgct ggtattgtcg
    15181 ggagggaaaa cacaaggcgt gctccgcgac aggctgttgg aacggagcga cagcgtcgga
    15241 aggccggggt ggtgggtgtt gggcacgcgc ggtagggctc cgctccgctt cgtccgggga
    15301 actcacgggg ctccagcctg ccctaccttg ctgcgagaca atccatgtgg tcactgcttg
    15361 aatttgagcg tgtcggcggg gggactgccc tgcaggtttg ccggcgggaa acgtttttcg
    15421 gctagatggt agaatggttg gcaaggaggt atcgcatgtg catgtcggag aagcggcctt
    15481 tgaccaaggc cggcggccaa gagcggatac gaaaccccgc acccacggcc gatcccacga
    15541 cccgcacgct ggtggtctat cagacccggc cggaggaagt tgagaagggc gacgtatgga
    15601 acccggagta cggcctgctg tgggtgccga aggattggga gtaccttgcg cccggtgacg
    15661 cctttgtcac gcgatttgtc aagcgtgggc ctcattgggc gctgaggggc aggttcaacc
    15721 gcaagaaggg ctatcgacgg aagctgggca tttacgctcc ctcggcgacc attcgggagg
    15781 cccaggccgc cgccgagcgg accgaggccg cccgtaagga gcagcgtgcc aaggcggccg
    15841 tcgttcggga gaaggccgag gaccgctatc ggcgggagtt tgagcaggca tgcatcgagt
    15901 tcctgaattt cagcaagcgg tatgccaagc tcgcggccag gatcgcccgc gagaccactg
    15961 accgggcttg tcagaagtac agcggccgtg tggggcgcac gcagaagctc gacttggcga
    16021 ccaaggcggc cctagccgtc cgggcgtgca ttcgacacaa atacacgggc tacgaagata
    16081 aggtaccgcc gttccctgat cctttattgg aagacgagta ccgcaccgcc cgccaaatgg
    16141 ctcacgagga cgtggatgcg tttctcgaaa ggcacaggga caagggcggg tgagggcgga
    16201 ggcggtggcg cgggtctggc tcgcggtccc ggggaatgac gaatggcgaa ggggaagtat
    16261 gccaggggct tgcgccgcct ggctacatac ctgggcccct ttggggcatg aagagccgtg
    16321 ttccattcgc tggtcagccc aagcggggcg gcaggtgctc ctgaacggat caggccttct
    16381 atcgcccttt cagggctaat ccgtcccgac ggaatgcata ccagaggctt gcgccccctg
    16441 gctacatact tgagcccctt tggggcatga aggccgtcgg cgagccgaaa acacagggca
    16501 gttgcgtagc ggaaggcaag ttgtcccttt gtctttttgc tgcgagcttg tggatgcgtc
    16561 aacaagaggc gacggagtca acagaggaag ctgtctcggc tctctccgtt atctctgttt
    16621 gctcctcgtt tcgacttcta ctcttgtggt tatcctgcat ccggtgccgg ttcgatggtg
    16681 gctcgcattg agcccttgga acgtttgagc cgccagtcgg gcccgtaggc gtggatctgg
    16741 tcgcgcttca gctcggcgcg ttccttggtg gtggtatcga cgatcacccg gccggtggtg
    16801 tccacttcgc gggccatcat atacgccttg tttttgttgt ggccgaacag cttgcccagc
    16861 atctcgatga cgtactggta ggtgtggtcg tcgtcatcca gcaggacgac gtgatacggc
    16921 ggctgacggc gcgggatggg tttggccggc ttttcaacgg gcttggccgg cggtgtcgcg
    16981 acggcaacct gattcgattc ggccatgaga ctcactcggc atctcgaggc acagtccaag
    17041 gcgaaccgcc tttttgcggg ccgcaagcaa agcgtaccgc gatccggaaa catgcacgca
    17101 agcggtacag atgtccgacc atagggtttc aagtcaaggc ttcactgatc atgcaggtca
    17161 gttcgacaat ctgaaacggc ttgcgaagca gacgtgtgtc agagggcagc ccttcggtca
    17221 gatcgacctc cgtgctgccg gtcatgagaa tggccggcgt gtggcgacct tcggcccgaa
    17281 gatccttcag acagtcaatc ccgctgcgct tagggagatc gacgtccaca accagcaagc
    17341 ggatcgtctc gccgtgctgc cggcaggcct ccatcatcga tcgaccatcc tttgtcctga
    17401 tcacgctgta cccatccgcc tccaagcgag tggtcaggac cgtgccaacg tactcgtcat
    17461 cctcggccag cacaatcttc tctcctctgc cagggggccg gccgcctcga ggccccttca
    17521 cctcgggctc aacgccgggc tcgcaggtgg gaaggatgac tctaaagaca gttccttttc
    17581 ccacttcaga ttgcacttcg acacgcccgc catgatcttt gacaatgccg tcgataatgg
    17641 acagaccgag ccccgtgccg gcaccgcgcg gcttggtgct gaagaacggt tcgaagatgc
    17701 gtgtctggat gtcggggggc atgcccacgc cggtgtcgct gaccgtcagg cagaccaatg
    17761 ccggttcctg ctcctttgat tcgtcggtcc cgccatcgcc ggggccgccc acgggcccca
    17821 cggcgatccg caaagtcccc ccgcggggca tggcgtcgcg ggcattcacc gccagattca
    17881 tgatgacctg ctgcatttgc gtctcatcgg cgcagatcca gatcggcgtc tggccggccg
    17941 tttccgcgac cagttcaatc gaagccggca gggcccgccg aatcatcctc accgtccgct
    18001 ccaccgtttc gcgaagatcg atcggcttct tctcgccggg gacttttcgg gcaaagatca
    18061 acagcgagcg ggtaactccc atcgcctgct gggccgcctc ccaaatcgcc tcgaggcttg
    18121 cgccgatgct ctcttgctga tcctgcggca actgttgcct gatcagatcg acgttgctga
    18181 aaatgacggt caacagattg ttaaaatcat gggcaatgcc gctggcaagc tgtccgaccg
    18241 cctccatttt ctgcgcatgg tagagctgct cctggaggac ccgtttctct tcttcagccc
    18301 ggagtcgctc acgaatctcg gcgaccagct cggcagttcg ctccctgacg agttcctcga
    18361 gatgctcgcg gtgtctttgc agctcggcct cggccgcgct gcgttccaga acatttctca
    18421 ccgtggcggg gagcacggtc agatggtggc catcgggatc cttgatgagg tagtcccggg
    18481 ccccgagctt catggcctcc accgccgtct cctcactgcc cgtaccggtc accacgatga
    18541 agggaacgtt tctgaattct cccagcaact cgagacagtt gccgtcggtg agcgaaaaat
    18601 cggtaatcac gatgtcgaac tgctcggcgg cgaggtgtct gcgggccgcc tgcgacgaat
    18661 ccgcaagagt atagtcatac ggcagccgct gcttcttaac aaaccgctca aaggccatgc
    18721 ggtcgagcag atcatcatcg acgaagagaa ctcgaagagc ggcggagctc atggtctttt
    18781 ctcctgctac ggcagttcgc taagcgtcca gtagaggtcg atggttcgaa ccacttcgac
    18841 gaactgacgg tagtcgaccg gcttgatcat gtacccggcg acgctcaggt tgaagctttc
    18901 aattcgatcc tgttcctccc gggatgacgt aagcaccacc acgggtattc gtttcagttc
    18961 ggcatcacat ttgacgacgc ggaggaactc cgccccgttc atccgcggca tgttcagatc
    19021 gagcaggata atgccgggac gctcacaggc agggtctctc agcatccgca gagcctcctc
    19081 gccgttcgcc gcgatgagca gccgattcgt aacgtgaatg tccctcaggg cgcgctgaac
    19141 ggtgatctgg tcaacctcat cgtcttccac caacaagatc ggcgtattgg gtcgcatgac
    19201 tgtcttcctg cccggctact tgggcaacgt gaacgtgaac gtgctccctt ttcccacttc
    19261 cgactccagc tcgacctttc cactatacaa ctccacgatt cttctgacca gcgtcaaacc
    19321 gatgccggtg ccctcctgct gatctcgggg tgtcagggtc tggaagatcc cgaagatttt
    19381 ctcgtgatac ttcggatcga tccccggccc attgtccgtg acgctgaatc gccagcgatc
    19441 gcctgcgtct tcgcaggcca cggtcaccag gcccgcgggt ttgtttatga acttgatggc
    19501 gttgccgatg aggttttgaa atacctgcgt catctgcgtc cggtcggcga cgaccacggg
    19561 aagcctgccc tcgacggtca cgtggacatg ggccggagga gacagcgcct cgatcacctc
    19621 gcgcacgaga aggtccagat cgatcggccg gcggtcttct tcaacgcgac cgatgcggga
    19681 atactgaagg attccgtcga tcaggttgta cattcgcgcg atccggccgt tgatgagttt
    19741 gagtctgttt ttaccttctt catcgagcac ggaagcatag tcttcggaca cccactgggc
    19801 caactgactg accgcgcgca acggggcctt caggtcgtga gagaccacgt aggcgaattc
    19861 attcagcgcc gcgttggccg cggcaagttg cgccgtgcgg tcccgcacgc gctgctccaa
    19921 ttccgcgttc agtttgcgga tctcctcctc cgcctgcctg cgccgggtaa tatcaatgcc
    19981 gactccgaag aagtaatcca gttcttgtcc cttaaacacc ggtgcgccgt gccactctac
    20041 cagcagcaga cgaccgtctt tcgtcataat gtggttctcg ttcagcgtcg gccgtccggc
    20101 caccacaagc tgctcgaaga ttcgggcgag tgccccgcgg tcggcgtcgg gcacgaaagt
    20161 ggtcatgtag tccttaccga ccacctcttc cgccgtgtag cccaaggcgt tcaacatcgc
    20221 ggggttcatc atccttgtcc tgccgtccgc tccgatggcg acaaagaaag ccggtgaggc
    20281 atggatcagg gtctggctga aggccatctc gtttcgcagc gcctcatcag cccgcttccg
    20341 tccggtgatg tcgcgaacga ccgccacaag gtgcggccgg cccgcgagga acacccggtt
    20401 gagagttacc gccgcgtcaa agagcgagcc gtcgattcgg tagtggcgcc actcgaagga
    20461 ctgcggctcc ccggcgtaag cggctgagat tttctcaagg gctttctgtg tggacggacg
    20521 gccatccggc tggaactcgg gcgacaggtc gtacgggtga agctgcagga tctgctcgcg
    20581 ggtgccgccg aataatgcct gcgttctcgg gttgcagtcc acgaaacgat cttcttccat
    20641 gaggaagatc gcatcgttgg ccccctcgaa cagtgagcga taccattccc tgccgtcctg
    20701 cgcggcttgt tcggaccgct tgaggtcggt gatatccagg ccctcggcaa tcagaagagc
    20761 aatacggccg atgtcatctc tgacggcgcg caggctgaac tggatgttgt ggagtcgccc
    20821 gtcgcggtcc acgtgggtgg tctggaaccg caccggttca ccgtcgtggg caactcgggc
    20881 gatggcctct cgcacctgac ttcgggcggc ggaatcatga cgccaccatg gcccttccca
    20941 gaacggcttg tgcagcagct cctcgcgaga aacacccgaa aactccgccg ccgggctgtt
    21001 gacatcgagc agcgtgcctt gcgggtccat gacgccggtc aggtgaagag actgatcaaa
    21061 gatagccctg aaacgacgag cggtttcgcg ggtggtttcg tcgtccaacc gctccgcccc
    21121 aagtccgccg tgcggcgcaa ccgtcccggc ccccgagtca cgacacggag ttcggttcca
    21181 gcagatggcc agcacgatcg ccaaggcaac aaccaacaga acccaccaga acagccggtc
    21241 gtcgagaccg tgcagctcgg tctggcccac gtaaagttcg tcccgagacc tcaagagtcg
    21301 accgacggga agccgctccc acacaggttt tgggacaggt tcgcagtcac ctgctctttc
    21361 gagccaggcc ggcaatgggg cccggagatc agccgagacg atggccgcag ggccgggtcg
    21421 cgcagagtcc tcttctcgcc cgacctgttc ttgccgacca gcacggctcg gattttcggg
    21481 aatgatccag accacggtga gcgactggag taattggctc gggcgaccgt cgcccaagtc
    21541 atccaacggg cggctccgag gcgggccggc atgggccaac gcgaattgcc caattccccc
    21601 aaagcagacg agcgtcgccc agcccaacag cagttggagg tagatgaagc attgtcttct
    21661 gaacattgca agccctcccg ccaacaggga gccgcctggc atgacgggcg gcgcgggcct
    21721 ggtcgccttc acagggaagc ccacaatcat gggcgtcacg aatctcgtca tccgcattgc
    21781 cggccggcaa ttcagcaacc ggcccgtacc gacaatgagc cgcgcggcct tcagctttcc
    21841 aagcagactt gaactgacag ctaacataca ggcccgcgag ccaattgacc agcgtcggcg
    21901 ccggagagct tgggggcgcg ggagttggac aaccttttcg cctgcctgac tgaaccttgc
    21961 cgacttgcaa gaaagccggt tgcaggtagg cttagacgcg gcatgacgcc gatgaggctt
    22021 tattcaatgg caggtccgac ccatacaccc aagccggccg aggagcggcc tcccgcaagg
    22081 attcacattc tgctggccca ggaggttcct gtgggcgtgg tgttccggcg agggccgtcc
    22141 aagcaggtct gcgtcatcgg ctggaaccgg gccaacgata cgtttatggt cggacagtgg
    22201 ctcaagggac ggatctacga gcgccgcagc gacctctcgc ctgacggacg gtatctgatc
    22261 tacttcgccc tgaagggcaa gccgggcacg gataagagcg tgtcatacac tgccttgtcg
    22321 aaggccccct ttctcaagcc catcgggctt tgggagaaag cggacagcca acacggcggc
    22381 ggcctgttcc tgaaccgcac cacctactgg atcaacgacg aacgcagcca caagaccgtg
    22441 actgtcccgc gcgagctgcg caaggttccc cattaccgcg gagagtattc gttcggcagc
    22501 gagtgcgccg gcgtttattt cctgcgactg caacgggacg gatggacgct gaaggaagca
    22561 ccgtccgggc cggaagggcg cagcttcgcc gtcttcgaga agcctgcggg ggccgggtgg
    22621 gtccttcgca aaaccgctca tgtgacacct ttgaactcgc ccgacgcgca cgaactcgtg
    22681 cacaccacgg ccggcacccg aattgacctg ccgggctggg aatgggcaga tatcgaccgc
    22741 aaccgtgtgg tctggagcac ggcgggcaag ctctttgccg cccagatcag cgagacaggt
    22801 acttttgagc cgaagcagct tttcgacttc aacccgctgc gttttgtggt ccggaccgct
    22861 ccctattgag gcgcggggat cgcggtcaag cccggccgat ggcatgacct tggcaatcaa
    22921 aaagcagttc ggcttcgggc aaatggaagg agctttgaag tctgcgattc agccggcgcg
    22981 gccgcttctc aaggcgggac gacttctgat tcatcgacgg gctgggcggc cgggcgggtt
    23041 gcggccctga tgaggatctg ccgggcgagc cgattggcca tctcttcttc cgctttgcgt
    23101 tgccgttcga tttcctcggg cgtggcggcc ggttgtgcgc cgggctcgga cgctgtgccg
    23161 accaattcgg cgacccggcc cttgacctct tcaaacgtgt atctctcgcg acggcgttcc
    23221 cggccttggg cgtcgatgta ggccaccgtg aaactgtcaa aggcaggatg ggccctgtcg
    23281 tagtagaccg tcatgatgac ccgaccgccc cacacgtcct tctcgcttgt gagattgagg
    23341 cgatcaactc cgcgctgatg gaagtgattg gtcagcacag tatccagcag caatgaacgg
    23401 tccaggcgct ccgggggtga caacatattc agccaactct cgtccacggt tttctggccc
    23461 tcgggttcca gccgctcgac cgcaggcacc gtcaagggcc cttcacaacc cagtaccccc
    23521 agaatcagta tgaccgagac gtttcgcatc gggattcctc cgagaaccca taatggacac
    23581 gcctaaaaga agatcatgac ccgattgtcc gtttttctcc tcgcaggccc ggccgtggca
    23641 acaggctaac gcaacgacct cgacccttca atgcatggag tcccgacaaa gcggaccatg
    23701 atccgccaag acaaactctg cttcgctgac tgcttctcgc tcactccgtc ttcggctcgg
    23761 ccaccctgcc catgaagaga atcgcaccgg acacgcggtc gcggatgagg aagacgaagg
    23821 ggtggtcggc gcgaaattcg gggatccgct cgggcatggg ggcggcggtg agcttcatga
    23881 ccacggcggt ggcggcggca gcctcggtgc cttcctcatc gacattgacg aaggccttgt
    23941 ggaccacggc ggagatgaac aggttcttct tgccgttcat gccggagaag tcggcctggt
    24001 tcccgtcaaa ggccttggtc atgcccagtg attgcaggac ctgtgcgagg ctgaattccc
    24061 tggttgcctt gaacttgggc aaagccaccc tcaccttctg cggcttgagc tgtgccagcc
    24121 actgactcag gttgttgggg gtaagccgct tctcgagacc ggccagcccg tttttctgtt
    24181 cgggcagcaa gatgatcatt gatagccggt tacccttgta cgtcagctca agcgcctgaa
    24241 ggttgtccat ttcagcgtag ccgagaatcg acgtctgatg catggtgggc acctggattt
    24301 ccttgccgcc agccgcgaag aacggggcag ggttggtgtc ctgcttcttg aacttggcca
    24361 gccactcggc cttcatgtag atggcgttgg tgagtaccag acgggtggct gcgttcagaa
    24421 caccctgttt gatcagttcc ttgatcttat cggccgtctg tttctcgacc cagtcgttga
    24481 tggtcttgcg ggccccttcg gtgtcgccca cgtagtcgac ctcactgagg ccggcaccgt
    24541 aggaccgctc ggtcaggtcg agaaactcct tcagaaactg ctcgcccttc tggccccaga
    24601 ggcggttggc gacgctcaac tggaaatcgc ctttcttgcc ggcttcgcct ggctcggccg
    24661 tgccaaggcc cgcctcgttc aggtcgcgga tcatctccgc gtaggccgag tgaagtcgct
    24721 ctgcgggcag atcaaagtgc agcgtcttgg ccatttcctc ggccgtgctc gtgcgggccc
    24781 cggcgtaggt catggccagg gcggtcgaga tgctgtaggg cgagaagaac aggttttcgt
    24841 cgccattctt gagcttgccg tagaggtcga gggcgaaacg gttgttggca tcggccacgg
    24901 ctttcacgtc agggggcggt gcgggccggg cgacctcgga ctgggccgcc ttcgcagcag
    24961 cagcggtcag catcatgaca agagcgacgg tggcagccga aagccagaag gcaggtctcg
    25021 gtcgcctcgg cgacctcggc catgccctac gacgcgttct gattgagcga atcatgactg
    25081 gctccttcga atgagcgtat cctgctgatt agacgcactc ggaccccttg tagttccctg
    25141 aagaccctgc gaatcaggag aaatgcaggg aacaaaccct ctgctgatgc gagagccctt
    25201 tctcgcaccg ctcctgggtg aatctttccg tccttagcgg cagcggcacg gaatccgccg
    25261 ccatggagga cggagatggg aatctcggtc cagcggtccc ggaccggcgg acccagggga
    25321 caagcccctg ggctcgtcaa gacaatgacg agtcgcggct acaagccgct cctacgcccc
    25381 ccccgtcccc cgcccctcgc ccctcgtccc ctattgcatg tccgcaaaca ccacctttgg
    25441 gtcgagtttc tggaaaagga tagccggctc gccaaggggg tggccggggg gaagctcggt
    25501 tgttgtctgg tcccaggaca gggcgccggc gtcgaggttg agcatcctga ggcacttgtc
    25561 ggccgagaag ggcaggaacg gggccatgag ggtcatcagg gccttgacgg tctgaatgca
    25621 gacgttgagg cttgttccgc aggcggccaa gtcgtccttg cgttgttgcc agggcttttt
    25681 ggcatcgaaa tagacgttgg aagcccgagc gaggttcatg agctcctcga tggcggcgcg
    25741 gaatcgcagg ccgtccagcc gtgcagtgat ccgctcggcg tgggcagcga tgttggccag
    25801 atggtcgttg tccacctgct ggcggttgcc gacggccggg accttgttgt caaagtactt
    25861 ctgggcgaag gtcattgtcc ggttcacgaa gttgcccagg gcgttgacca gttcaccgtt
    25921 gttgcggctg ataaagtcgt cgacatcgaa ggtagtccgc tgagtttccg gcgcgatggc
    25981 cgtcaggtag tagcgcaagg ggtcgggctc gaacattttg aggtattcct cgatccagat
    26041 ggccgtccca cggctcttgc tgatcttttc ctcttctttg ccggggaact taatattcag
    26101 gaaggagttg gcgacgacct gccacggcag ttggaacgac ccctcggcca tgagcatcgc
    26161 cggccaggta agggcgtgaa aaacggtatt gtcttcgccg atgaaatgca cgatccggca
    26221 atccgggtcc ttccaccatc gctcgtaggc cttccagtcg ccgtccacct gccggcaaag
    26281 ctcggccgtg aacgacacgt agccgatcgg agcgtcgaac cagacgtaga gcaccttgcc
    26341 ggccgcgtcg gggtcattga gcggcaccgg cacgccccag tgcaggtcgc gggtcatggc
    26401 ccgctctggc aggccttgtt tgatctggcc gagcgagaaa ttgagcaccg tttctcgcca
    26461 cggcgggcaa tcgccctgcg gctgtcgctt ggcttcgagc caggcacgca gtctctcctc
    26521 gaactgcggt aactgcatgt accagtgggt ggtctcgcgc ggctcgggct tggtgttggt
    26581 gatggtgctg accgggttga tcagcttcat cgggtcgatg gacacgccgc atgcttcgca
    26641 ctggtcgccg taggctccgt cggcaccgca gttgtagcat ttgcccttga cgtagcggtc
    26701 gggcagaaac tgatcagcct gaacgtcgta aagctgcatg gtggtcttct tgacgaagta
    26761 tcccttctcg tggatgacgc ggaagaagtc ctggctgatt cggttgtgca gatcgacgaa
    26821 cttgggctgg tgggtgccgc cgtagatgtc aaaccggata cccagcccgg cgaagtcggc
    26881 tgcctggcgg gcgttgtaga aggcggtcag ttcctcgacc gacttgccct ccttgcgggc
    26941 ggagatcagc gaggccacgc cgttgtcgtc gctgccgcag atgaagcgga cctcgtcgcc
    27001 gcgggcccgg aggtagcgga cgtaggtgtc ggccggcagg taggccccgg cgatgtggcc
    27061 cacgtgcaga cggccgttcg agtagggcaa cgcggaggta atcaggaatc gacgtttgct
    27121 catgttgatg ctcacttctg gtcttagctg aggcttttgg caggtctggc cgccggtggc
    27181 tgctcgaccg tgccctacct cgcccttcga cgcgaaatca aggattccgt cggtatcata
    27241 tcggcagtca tgttcttgaa cagggagtca accacgccga agaaggcccg ctttacgcgg
    27301 gccgacgcat gtacataggg gggaccgatc tgggtaaagg tgctttcctc atgccgacga
    27361 taatactcga agaagcgcct ggaggccagg ggttggtagg gtccggggct gctgggctat
    27421 cagaccgcag cgggcatctg cacgcaagtt tacatggctc ggggcggacc tataatggtc
    27481 ccaatccgct cggtcgagcg ggttgtccag taagaaggac gattctcatg gccaagctag
    27541 gtgtcaatat cgatcatgtg gcaacggtgc ggcaggcacg gcggacgatc gagccggatc
    27601 cggtctgggc ggcggcggag gcgcagcttg gcggggccga cggaatcacc ttccatctgc
    27661 gggaggatcg ccggcatatc aacgaccgcg acgcagcgct gctcaagcag acggtcacct
    27721 gcaaactgaa catggagatg tcgattgccc cggcgatcgt gcggattgcc gagaacctgc
    27781 ggccaaacca gtgtacgctg gttcccgagc ggcggcagga actgacgacc gagggcggtc
    27841 tggatgtgat ccgcttcaag cgtcggattg ctccggttgt ccagaggttg caccgcaagg
    27901 gcatcgaggt gagcgctttc attgagccga ttgccgaaca ggtgaagatg tgcgccgacc
    27961 tggactttga cgccatcgag ctgtggaccg gcggatacgc ccatgcccgg ggggcccggg
    28021 cccgcaagag gtcgctcaag cagctcgaga cggccctcga actcggctgg gaacgcgggc
    28081 tggtcgttca cggcgggcac ggcctgacct atgacaacat cggcccggtg gccgagatgg
    28141 aattcgagga gttcaacatc gggcactcga tcgtcgcgag agcgatcttg gtgggcatgc
    28201 gtgaagcggt tcggcagatg aaggtgctcg ccaccggcgt gttccagtaa tcggcggcgg
    28261 ggaggccaaa acggcgatga aaggtgtggt gagtacagcc gctgttctgc tggcgactat
    28321 cgccacgacg ttgtccgcac aaccgacttc gagcgcgtcg gcaccggtga ctgcgactgc
    28381 gtcggtaccc gcgccggtcg agaccgcggc accggctccg ccgccggtcc gggcgacatt
    28441 gcccgcaccg agccctgcta cgaacgctgc ccccgcaccc ccgacggctc ccgcttcaat
    28501 gccgacgacc gctccggcgc cccccggcag cctggccatc cgggagggtg acgtgatcgt
    28561 cttcctgggc gatgaattga gcgaaacgcc cgacgcccgg cgaggcggtc gaccgacgtt
    28621 cccgcaactg gtcgagacgt ttctgacggt gcgttatccc gagctgcacc ggagcccctc
    28681 ggcggacgga ccacggccgg tccggtacat ctctgtcggc tgggcaggcg acaccgctgc
    28741 gcgggctttg ctgcggttgg aacgtgacgt tctcattcac aagcctacgg tggtggtcgt
    28801 ttgcctggga atcaatgacg ccggttacct tagtttcgtc ccggcccggt tggaggcgat
    28861 gcagcgtgat ctaaccaaga tcgtgcagaa atgccacgaa gccggcgcaa gggtctggtt
    28921 gatgtctccg ccctgtgtgg aggaggaacg cggcaagaaa gcgcgcgtgg ttcgtgacgg
    28981 acagcgggcg gtggccgacc tggaagtcat ccgatacaac gagacactgg ccaagtatgc
    29041 ggccgcgatg agggagattg ccgcggccaa tccgccggcc ggtttcgccg actggtttgc
    29101 tgaagcgtcg gccgcgagcc ggcaggttcg cgcgtaccca ggcgaggact ggctgactac
    29161 cgatgggcga acgctgactt ttcacgggac gacgatcgga gcaaccacgt tgctgcgggc
    29221 atggggggcc gagccgattc gggcgctgct tgaactggat tggacggacg gcacggcacg
    29281 gatcagcacc caccaagggc aatcgtcaac catgctcgtg cagatcgcgg atgatgagaa
    29341 acgaattctg tctgttgaca acctgccgct gccatggccc ttgccgagtg aggccggcgt
    29401 cggattgcag agcgggtgga aagccgctga tctgtgccag atcatattgc gcgtgcccga
    29461 tccaccagag caggggatta cgctagtcca ggaaacggac gaccatgggg ccacagccca
    29521 gtggcctatc actgcggccc aacttcaggc gggtttcaac ctggtgacgg ccgagccgtt
    29581 gcgttcgccc aagggcgtcc gcgatctgta caatctcatc gtcaccaaga atcgcacgcg
    29641 cgacacgatc tggcgacgcc tggagctgtc ggcgccgcag gagcccgaac tgaccgacgg
    29701 ccatcggcaa ctcatcgagg catggaaggc ttacgttgcg ggttacgaac gaatcatcta
    29761 tcggtatccg aagacgttca gcgcccggtt tacattgagc gagacgaccg agtccgagca
    29821 tctgcccacg agccagccga cccgcttacc acgggtggtg ccgtcatcac acaacccgat
    29881 cccggccacg ttgccggctg ccgtgcatcc ttgaactctt ccagcccggt cgcgggggac
    29941 tccggaacgc ctttagtccc tcccgtcgcg actggtgcgc attttgcgcg catgtttctg
    30001 gatcgcagag cgcttgggtt g
//
LOCUS       DAOTPW010000031        29738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1630_length_29738_cov_5.434862, whole genome shotgun sequence.
ACCESSION   DAOTPW010000031 DAOTPW010000000
VERSION     DAOTPW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 29738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29738
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1630_length_29738_cov_5.434862"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1650)
                     /locus_tag="P5316_04470"
     CDS             complement(160..1650)
                     /locus_tag="P5316_04470"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HRR84243.1"
                     /translation="MFRRAFCIATACTTMTLVAQAAETAPASRPVVCEKADGYRGIWS
                     AGGELPGEYKYQFSGGLATFSPQHVPMAIHADKPDKTFFVYGGMPADGHQIEENRRLL
                     IMVSCFDHQTGTAARPRILLDRQTDNAFENPTLSMDSVGRPWVFVGCHGTARPSYVFR
                     STEPYSIDSFSLVAETTFSMPQPWYLEKQGFILVHTLHDGPINRLCAMTSKDGMSWSQ
                     PNVLASIEAGHNHVSWRYKDKIGIAFDYCPRQGAGNARTNLYYMETRDAGKTWVNVKG
                     ERLDLPLSTVDNKALVRSYEDTRRLVYVKDLTFDARGNPIILFLIARGYRPGPLSSPR
                     IWTTARWRGRDWEFAGVIRSDNNLEAGCLFIDRMDRWWLIAPTDKGAAPFYLGGEVVA
                     WFTRDQGASWARWTFTEKSEFNHTGVHRPVDASPGFWVFWADGDARQPSPSRLYFADR
                     DGIVYRLPTQMTEEFEKPAVMPIPSARRPARTSVSDPATEATTQEE"
     gene            1818..2939
                     /locus_tag="P5316_04475"
     CDS             1818..2939
                     /locus_tag="P5316_04475"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRR84244.1"
                     /translation="MNTRLIKIDRSADCGAQLREAAACLAAGGLVVFPTETVYGVGAN
                     AADPDAVARLRAVKQRADTKPFTVHIGSRSAVERFVPGLSGLGRRLAEKAWPGPLTLV
                     FHVADIEAAPVVREISMGCAENLYHNGTIGIRCPDDRVAGDLLMAVRTPVVAASANPG
                     GEAAPVEAEECLRALGGQVDLVLDAGRTRYAKPSTIVRVDEKGYELLREGVLDERTIR
                     RLAHVTFLLVCSGNTCRSPMAAGLLRRLLAEKLGCAETELEARGFTVESAGTSACTGA
                     PPSVAAVRALEFRGIDISGHRSRTLTSDLVSRADYIFAMTASHATMVTSLGPGAGTLV
                     RTIDEEDIEDPVGGNDEVYSQCADRLEKALRNRLKEIAL"
     gene            2936..3457
                     /gene="rpiB"
                     /locus_tag="P5316_04480"
     CDS             2936..3457
                     /gene="rpiB"
                     /locus_tag="P5316_04480"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007069121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRR84245.1"
                     /translation="MRVAIGSDHRGYAAKEKIKSMLASKGYEVLDMGTDSPASCDYPD
                     IAYPTCKMVTSGKADLGILLCGTGIGMSISANKVHGIRAACCHDELTAEMARRHNNAN
                     VLCLPADLIGEELMRRIVDVYLNTGFEGGRHERRLRRIAEYEMKEGQNVGEAMKQGSA
                     AALSEHGKATRKM"
     gene            3668..4528
                     /locus_tag="P5316_04485"
     CDS             3668..4528
                     /locus_tag="P5316_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84246.1"
                     /translation="MLRKTIKYGVIGLTGLLVTAGVLFGTDLGSYLWTSTRTVRTAVR
                     DSVPIEFELQRARDLLEDIIPEMQANVGLIAKEEVEIANLKEEIQRGQASLAEEKARI
                     TKLGDTLKAQQVRYVFGGLEYTHDQVKAELARAFDRYKEAEVVLAGKQRLLDTRQKSL
                     QAAMQMLERTRSEKARLEDRIAALDSQHRLLKAASTTSQVRVDNSKLAQTEKLIGDIK
                     KRLDVAERVLAHEAQFVRPIEIDVISEKDLLAQVEEHFSGKPAQVGAAAAEATSPIAM
                     GQAGARTPTP"
     gene            4600..6180
                     /locus_tag="P5316_04490"
     CDS             4600..6180
                     /locus_tag="P5316_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84247.1"
                     /translation="MLILKIKQAATALKDGRLDEAYELARAEDFRTHREGQDLIGQLV
                     RKLVARGQDHLSAERLGQALADCEKAERLGGNLPETAALRAAVTDAIANRQQVERRQA
                     GLVTMARQHIRDGWLSLGEQVLADVASETAETLRREAEARRVRAEQALNRAEQALNKQ
                     DWALAIESLREARQAHASNPQTADLMDRATVLVADQTSKAINQGRLDAAEAMLEHLAS
                     LTKPTLQIQELTRCLGECRSAASCLERGQPRQAAEILHRLSAAYSEAAWLTEACRQAE
                     QAAAATENLRRGPLGWFTGSGPPVRTPVPTYEADRTDTMPPAKPAKGHAAGGVVPDRF
                     MLQIDTVGGFLVLRQPRVTVGPISSSRRPDLALLAEAGLPVVTIERQDDDYFFSSETP
                     VRVNDMPVQRKLLSDGDQISLGPRCVVKFTLPSAASTSAVLHLSGCRLPSGDARRVIL
                     MDKSIVLGPGSLAHIRADQMDAPAVLHIRDERLLVRSTTVVKIDGRPIDDRVGIPVGA
                     CACIGPISLAVARAEVRA"
     gene            6239..9397
                     /locus_tag="P5316_04495"
     CDS             6239..9397
                     /locus_tag="P5316_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRR84248.1"
                     /translation="MDGFQYKFGDQPLEGYTIQRAIGRGGFGEVYYAVSDSGREVALK
                     VVQGYEDIELRGVSQCMNLKSPHLVTVFDVKRNADGRPFVIMEYVAGPSLRELLQEAP
                     SGLGIQKAAFFLREIAKGLTYLHDRGIVHRDLKPGNIFYEDGYVKIGDYGLSKMIASS
                     RHSVQTITVGTVHYMAPEIGQGNYDRSIDIYALGVVLYEMLTGQVPFFGSSHGEVLMK
                     HLMAQPDLSQIEEPFASVIRKAMAKEPAERFQSVQEMVEAVFGAEHIQQSVSHFRPES
                     LSMVARRVAQKVTVGGSGSSAEHAQGLGPTAPSGEAGSSDVWQDLSKHIDDFGEKVGK
                     WGERLSDRMAAVGDRVGRQFGQKPAALPYANAAAPASDGVIDTALDPLDRRARKRLWV
                     VAALVMGAGTGIIEATSHNGGPAIELSIFATVAILLASLGIFRAGRLLAERMQKERGL
                     GRYVAFGGAAALGALAAVFFGEIGPFRKQIHAALDDNALPLLASCFFIDWLGRMNPVR
                     QQRISFESAFTAGLLGFMVSIFTDASAIVMIGVLAGLSMAVQAGSAFIPPALRKQLGI
                     TRGNGQHDHPRPPERGTPPAADRTPQIQNAEAHRPRPLPVAVPPGIEVPLFVRALSLL
                     AFAVLLGGGIIFLILAGMASPSNLDYFAGCVSAGIGALLFSVFALVKATQRYLVSWWS
                     GLIKPVLMIALLVTGIASAVALGSMQLHNDDEMIAIAVIVFAAVFFIFVACTTNRMIH
                     AVLGQPFPDAGRATPRAEHAAGVSPRGRLWAMLLALLFFACPIGGLHRFYVGKIGTGV
                     LWLVTFGGFFIGQIVDIIMIAAGQFTDKQGRPLLTWTNPNEFKGRPTVATEISPPPAS
                     AVATPMVAPGDVRPLELGSLMLSGVGMLVLLAGVLTGLLVAVHLPAIIAAGIPDQSLE
                     QELTREFGYSNWPQLLERIGTFVAVFLFLASCAILLLARRKSGGAHCLRAVLGAVAIG
                     VSLAAFADATSRIQWATVSTLLQAERIGPAIDAALGAIDKEGATISAVLLLISIVLLF
                     WPADRRQADVTTVGGDAVSARR"
     gene            9402..10835
                     /locus_tag="P5316_04500"
     CDS             9402..10835
                     /locus_tag="P5316_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84249.1"
                     /translation="MTHVEALPVGISCFGIFLLIALAGLVVLILGNRGARRVLAGLAI
                     GVAVVVVFGLLAMFWGRSSVHYSTSSPTLFSEAVYPPEDEAMESHAVRGVTIGSNGVV
                     VREGSSSIVVNGNGVSVTDEWIDAGQADFEADVYPSERSAAVALARKAIKSWDRVLPG
                     GQEPTQILIRGDSELEPAVVPAVLQALRAKLGDDRVEMDKSPQEAQIEEVERVLVSPG
                     SAPSLPEADATVRLAINGNFSYVHGGRGVIPSQKRNGTIELVLSGPAGKSTHTAAFVN
                     KPWVEYGIDRAAQVSGRQPMVARSKRLCPTRGEAEQQAIAEAAKNLVPLVRTALERLE
                     SSGASQSNRGAIAIQQRRPDPELYEKIRAELLRGTGVDDQFTQHIHRPYGDVWQHALL
                     VDGSDGQITAYAQTFLPQIVQQQKTALRIRKSWARTIVGLALLTVLIAVVYAFLNAAT
                     RGYYTWLLRGSMVLLVLGGAVVLLLMT"
     gene            11003..11929
                     /locus_tag="P5316_04505"
     CDS             11003..11929
                     /locus_tag="P5316_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR84250.1"
                     /translation="MANILTEAEQYLLDQIRQGRPQAWEQFVDRYQGRLEAFARSKLR
                     TPADAEDLVQDAFMSFVQSLGAFRGQASLETYLFAILRRKIINWGRGRKSSICLLQDA
                     LPGGGHDDSGDAADRLAAPDQTASWYVRRDEEQERRHEALAQAMQELVDGFKQSLNFR
                     DLKITEMLFYCRLRNKDIANIAGVRENHVAVTKHRYLKQLAERMTAVLGNSSAACDDS
                     FLTAEGSDAMLCEIWQERRLSCLKRSTIGAYLLGTLEPEWQDYVTFHLERLGCQFCRA
                     NLDDLRRQTADDGTRRLRDRIMESTVGFLRKP"
     gene            complement(11953..13296)
                     /locus_tag="P5316_04510"
     CDS             complement(11953..13296)
                     /locus_tag="P5316_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRR84251.1"
                     /translation="MTDVSSPPREKPALAREEHALLRTMGFWSLAIYGVGDMLGSGIY
                     ALIGKAAGVMGNAIWLAFLASMFAAMFTGLSYACLGSRYPRSAGAAYIAQRAFGRPML
                     SYLIGLAVVASGLTSMATASRAFAGYFMELSGPLPAPLIILGFILLLTIINLRGMQES
                     TWVNILCTTIEGGGLVFIVIVGLRYWGGVNYLEMPPHATVGPASLVLQGAVLTFYAFV
                     GFEDLLNVSEEVKDVERNLPRALVTALFVVAGLYTAVSITAISVVPHAELAESGGPLV
                     EVVRQAAPWCPPVLFTGVCLFAITNTALLNYIMGSRLVYGMAHQGLLPAVLGRVHFKR
                     RTPDSAILILMLIVIALAMAGDISVLAKATSVLLLGVFTIINLALVVLKRRQGEPKGT
                     FEVPLFVPVVGAIICVVLISHAQIRELLIALILLAGIAILYVLVSRRHRRTGLPD"
     gene            complement(13444..15495)
                     /locus_tag="P5316_04515"
     CDS             complement(13444..15495)
                     /locus_tag="P5316_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR84252.1"
                     /translation="MDVDAEGQLASYSQTGPGQCRGQIRHIRIERSVEMTQDQQDYRH
                     LTRRDLFLAAGSGAALTPLLGGCRAESSGLGQAGQADAAAVFESGSRLTRSNKIEADV
                     VIVGGGMAGVSAALAALNHGASVVLVHDRPVLGGNASSEIRLHILGADEHGARSDTDS
                     RESGIIEEIRLHEVVRNPHRSYGVFDLLLYEMVCRRQGLTVLLNTSCCGAVMASKSRI
                     AAVLAARPGTQDVFTISGKMFIDTSGDGRLGAEAGAEFRTGREGRTEFGEDLAPPQPD
                     NQMLGSSILFTTRRYDQPIPFTPPDWALRFPTCEDLPYRSHGSWEWGFWWVEWGGHID
                     TIRDNERIRDELIAAALGVWDHIKNSGKHPESANWALDWFGFIPGKRESRRFVGDHVL
                     IQQEVQRGEQFEDGAAYGGWPIDLHPSIGIHSKEKPCLQIRVPMYNIPLRCLYSRNIE
                     NLFFAGRDASMSHVAFGSTRVMATGSVMGQAVGTAAVMCIRNKCTPRELGRQAIHDLQ
                     QQLLKDDAYIIGATNSDSLDLARTAEIRASSETVQGRAANVIDGVTRGVGPATHRWIS
                     DPQQKMPQWIELRFRKPARIREVHLTFDTALNRSMTLTLSDHQNAKMIRHAQPETVRD
                     YELQILDKGGAKTVAQVTGNYLRKKLHRFDPVTASGLRLNINATNGDASARVFEIRAY
                     E"
     gene            complement(15468..15641)
                     /locus_tag="P5316_04520"
     CDS             complement(15468..15641)
                     /locus_tag="P5316_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84253.1"
                     /translation="MLHEKRCIGIAAYGRDGCVAGNTRGAGFHARQAIPRSDATLRRR
                     DVNKPWTLTPKGN"
     gene            15671..18094
                     /locus_tag="P5316_04525"
     CDS             15671..18094
                     /locus_tag="P5316_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR84254.1"
                     /translation="MMTHGRLVGDGFVQTGRGLAVLAMGMMLLAGCAGREHYDRGRML
                     MASGQYDQAVTAFDEALAQSPGNSRYHNGLLEAKSLAADQHVKTATQFVAEKRLSDAQ
                     KELDTALKLVPAHPEGVPLAAQIAPQIAECEKTIAEAREALARQEWTEGARLIAEAGR
                     IDRSHPQLELLRKEAADTVTARQLLAARRALGARDWDAALAACAQVRRVAPDNTEVVG
                     IEQQVKDRREAQRVFDSAKAMIDAGNSSGALGPLQQAAGLWPDNDEIVSTLDRVKGQV
                     VDKLSTRSRDEARAGRFQAALQTLDEAMVLEPNRESLRQQRKQVLDGWIAGLMEDYRR
                     HKAQGAWELAWVDAVQALALAPTQPEPVVQACLTAEEGIRRAIAYNLNVLLAPSDERR
                     LDDIMAVCTVLLEELGDQRPDHVCLTEQSILSKLLAEFAVSSADVNNRDKLQAVGRRL
                     RGPNVFLFVNMDIVKVNVGNPESAASGTSRLDLRMNMVDVSSGRLLWKCVEMLPLEGS
                     ASPGGTAATARPVAVVHASGPLTADGIAVLRPVLRARVSDMYRGHAGSFLQAAGAATG
                     DTATEFNVRFLFDLVSMPDAQTLGSVLDAVFGSVVSGDILAACKKIASERLALAKRVA
                     ARDEHPAEKMSGPAILPASRPAVSTATAPVAHQAPAVPSASKPATAAPASADVRSFPA
                     TQPRPVPVPASTSAASTAPAEDRGPVRVFQGVVSRDDDRFTKELATVDGIVVKLLDTD
                     ADPLDADIEIRVGKRSKKYEDKPQGARIGGYGESGRAYVVVILKIDDETETVHFAVER
                     VDTVPQQQP"
     gene            complement(18099..18827)
                     /gene="recO"
                     /locus_tag="P5316_04530"
     CDS             complement(18099..18827)
                     /gene="recO"
                     /locus_tag="P5316_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRR84255.1"
                     /translation="MPAIKDEAITLKRLDYSETSQVLVFLTREHGCRRLIAKGIKRGT
                     SKRFATGIDLLERGQVAFIAKFQSDASLGTLTEWQQLDAYMGLREDLQRLYGGQYAAE
                     ITTAMVEEADPDADLFDALRDLLGGLACGAEPLPLLVGYQCALLRSAGLWPDLSRCVM
                     CGKPAPAGRAGFLAVHQGGLACRNCEPGIVDKRKVSGATLDALREARLTPATARDAFD
                     LLNYTITHIIGRQPTLARFVLTGK"
     gene            complement(19129..19539)
                     /locus_tag="P5316_04535"
     CDS             complement(19129..19539)
                     /locus_tag="P5316_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84256.1"
                     /translation="MASNSGTFLRRWEIATGEKEKRQGRRRTLRCSVQLFNDAEDNGQ
                     PPLTAECLNIGDGGLFAVLPSNVGVAIGQRYTFRLIIGERGPEPGHRQCVTQKGEIIR
                     LELLLGQEGYADRIGIGVRLFGPRSGIVPMPTTR"
     gene            20795..21763
                     /locus_tag="P5316_04540"
     CDS             20795..21763
                     /locus_tag="P5316_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR84257.1"
                     /translation="MRRKRSGFTLIELLVVVAIIALLIAILLPSLARARELAKRTACA
                     ANVSGMGKGLYTYATENADAYPLAAPNTWTHGNPKTAVTYWNMTGNRGGPSAGTGITP
                     ANTGDPAAANPLMSQLSTTRNLWQLVKTGGTSPRSFICPSSSDNADTIDNPADYWDFP
                     AKAGDGTVNRGYVSGQDNEGCISYGYQVPYGDQGRPSTEVDQRMALVADKGPYCFKVN
                     GSPPPTSWNITEAQNQSPDMWMPYNSPNHGGQGAGEGQVVLYADSHAEFQNKPIVGVG
                     FDNIYTAWPSSGNLLADANLRTAGVKPASNNAQLVPRVNTDSLIYP"
     gene            22996..23485
                     /gene="rnpB"
                     /locus_tag="P5316_04545"
     ncRNA           22996..23485
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5316_04545"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23578..24900
                     /locus_tag="P5316_04550"
     CDS             23578..24900
                     /locus_tag="P5316_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRR84258.1"
                     /translation="MVLHVLRVIFVALVAIVAMRVAEYCWRVMPAYAILSFALGLACL
                     VIALDIFIPQKSLLAISGLFFGLVVGMVVAFGLGLILDAVVLPYKSTLTKVLESDAIG
                     GFWPSAPIPEGSYPEEDTAARRSGWDSDGLSGSEGGMFRKLLWAVLGPHRGGSAQRPA
                     TAVQVERQSVGEARFKEMMSSIKVLVGVVCCYLAVSFILQTKDDIRFVIPYVEFVKQR
                     KGEHPLILDTSVIIDGRIIDILKAWHVDSPVIIPRFVLQELHAVADSGDKLKRNRGRR
                     GLDVVNKLQELETIDLQFQEARTTREEEREGVDHRLVFLARKMEGRLVTNDYNLNKLA
                     QIRGVDVININDLANALKPVFLPGETMQVKIIKPGEEVGQGVGYLEDGTMIVVEGARD
                     KIGQSVTISVTSVLQTSAGRMVFGRMGDAPPPPDRSRRRQSPPNRPST"
     gene            complement(24931..25758)
                     /locus_tag="P5316_04555"
     CDS             complement(24931..25758)
                     /locus_tag="P5316_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84259.1"
                     /translation="MRKSLTGLLLLAPLSGCATPINDGLRIDWRKGERDILTIRSDRL
                     PGGKIETWYLEAFCRRGSTDRDWSQTTIPFESQLVAADPDGRWVKLRHIVDGKVVIDH
                     EIRAGYDEVDIHMTITNQSNEFVDLDWAQPCVRVGDFTGRGQDDYFQKCFIFTDQGLT
                     RMDQTHRETKARYTPGQVYVPGGVDLNDVNPRPLSRTRPVNGLVGCFSADEKMILAMA
                     WDHVQELFQGIIVCIHSDVRIGGLKPRQTKIRHGKYYIIPNDVPTLLARYERDFGGK"
     gene            25809..26432
                     /locus_tag="P5316_04560"
     CDS             25809..26432
                     /locus_tag="P5316_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRR84260.1"
                     /translation="MIESLTGVVMAERMTCRLGRESYPADGEDAVLAGGLARRDPVVF
                     DQLVETYQARVARLVCRLLGWPQDVDDVVQEVFLAAWKGLPRFRGQAELGTWLMRITI
                     NKCRGHRRKEMLRLRRFWRSKERQQMRNAVSADGATLDAENQAEVRRAVRRLPARYCE
                     VIVLRYLEEMPVERIGQVLGLTRNAVEVRLHRARARLRKELGGLLEE"
     gene            26838..27437
                     /locus_tag="P5316_04565"
     CDS             26838..27437
                     /locus_tag="P5316_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84261.1"
                     /translation="MSDERLQQLLRQADAAFGGPRVSSADLPLRVRQRARRQHRAKVF
                     GTATAAVLVLSLTTAAWVNRYGRSVPSPTPIVADDGSKPASVAELQSHIEQLSEEIRT
                     GCQAVHEVLRRQEVEQRLAALRRQAEADPLKEINDQIDRAALTMVYQADRMYRELNLR
                     ESAIASYERTIKLFPKTHWAEVAKDRLAELKTAGNGDAI"
     gene            27434..28978
                     /locus_tag="P5316_04570"
     CDS             27434..28978
                     /locus_tag="P5316_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84262.1"
                     /translation="MKTGTLETNVVISLFVGGLAASAWGQAVPSPSMHDPDSPVNCVV
                     KIACDPALLPLNPQSVGALLESRFVAGEALKQTLPPEAFLSYTVEFRPLPGGTIAAPV
                     GVTPGMPMMDDPLPQPGGVFLPAPPGRDGMGGYGDEGGGGERAGSRSGGPSGSNAPAH
                     GGRYGGYGGGGRTGPAGGPWAGPEKPDAPSGGTPMPAGAVPDRSVAPGGPRPGGRPVM
                     VDIPEPGPGEGSIIGQIEVQIDGAEPHHNEAFLRAICERLQQALYGLWESEMSSIGRQ
                     LEMSRDELLRAEKKLRELQERRRDLLGAGQVDLTPEAVMDQIRQLDRERQRMEMDLLG
                     QQARLKAVQQQIQATSERSLASPGGDEMIKPLQAKLEVLRNELKRVEEMNQRGMVPGG
                     DVEKARVAVLEAEVQLVEARRKAAEVGGVEVLGKLNTELAMLSVNVAETEARLTSLRE
                     QIEKARQLVGVVDEYEMRVLMEMPHARRAYESSKLRVEELEQRIRTAVAPTVTVIGGA
                     TSKPAK"
     gene            complement(29166..29519)
                     /locus_tag="P5316_04575"
     CDS             complement(29166..29519)
                     /locus_tag="P5316_04575"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRR84263.1"
                     /translation="MSLIRIWLDEDVRPLLGAVLRERGYDAVSAVELGRLSIPDADHF
                     EYCAANGQALLTFNIRDFVPLARRAIEEDRHFSRLIVSERLPFSQLWRRTLRLVGTLQ
                     GEQLADRMIWLADYR"
     gene            complement(29519..>29738)
                     /locus_tag="P5316_04580"
     CDS             complement(29519..>29738)
                     /locus_tag="P5316_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84264.1"
                     /translation="SPFRRDWCAFCAHVSGSQYAWVAARKKAVRLREFPQLSLPAVYD
                     ALSYYYDHQAELEAEMEDNTEACWREEM"
BASE COUNT         5671 a         8820 c         9562 g         5685 t
ORIGIN      
        1 gagagtactc ggagcgatgc cccggagcaa tttacacagg agttgacact gcctccctgc
       61 ctccggccgg atatagctcc ggtgaattcc cgacccggtt gtgatccttt ggcctacgtc
      121 ggcttggata ggcggttcac gggaagacgc gtgcagacgc tactcttctt gcgtggttgc
      181 ctcagtagct ggatcgctga ctgaagttct tgccggtctg cgggccgagg ggatcggcat
      241 gacggccggt ttttcgaatt cctccgtcat ctgagtcggt aagcggtaca cgataccgtc
      301 gcggtcagca aagtagagac gtgacggcga gggctgccgg gcgtcgccgt cggcccagaa
      361 cacccagaag cccgggcttg cgtccacagg ccgatgaacg ccggtgtggt tgaactcgct
      421 cttctcagtg aaggtccacc gggcccacga ggctccttga tctcgcgtga accatgccac
      481 cacctcgccg ccgagataga agggcgccgc ccccttgtcg gtgggggcga tcagccacca
      541 gcggtccatt ctgtcgatga acaggcagcc ggcttcaagg ttgttgtcgg atcggatgac
      601 gccggcgaat tcccaatcgc ggcctcgcca ccgggctgtc gtccagatcc tggggctgct
      661 caggggtccc ggccgatacc ccctggcgat gagaaaaaga ataatcgggt tgccccgcgc
      721 gtcgaaggtt agatccttga cgtagaccag ccgtcgggtg tcttcatacg accggaccag
      781 agccttgttg tcgacggtgc tgagtggcag gtcaagtctt tctcccttga cgttcaccca
      841 ggttttgccg gcatcgcgag tctccatgta gtagagattg gtgcgagcgt tgcccgctcc
      901 ctgtctgggg cagtagtcga atgcgatgcc gatcttgtcc ttatatcgcc aactgacgtg
      961 gttgtgaccg gcctcgatgg acgcaagaac gttgggctgc gaccacgaca tgccgtcctt
     1021 gctggtcatc gcgcagagcc ggttgatcgg tccgtcatgt agggtgtgaa ccagaatgaa
     1081 gccttgtttt tccaggtacc acggttgggg catggagaat gttgtctcgg caaccaagct
     1141 aaatgaatcg atggagtacg gctcggtgct gcggaaaacg taggacggcc tggccgtgcc
     1201 gtggcagccc acaaaaaccc acgggcgacc caccgagtcc atgctcagcg tcgggttctc
     1261 aaaggcgttg tccgtctggc ggtccagaag tatccgcgga cgggcagccg tgcccgtctg
     1321 gtggtcgaaa caggaaacca tgatcagcaa ccgtcggttt tcctcgatct ggtggccgtc
     1381 ggccggcatg ccgccgtaga cgaagaaagt cttgtcgggt ttgtcggcgt ggattgccat
     1441 cgggacgtgt tgcggcgaaa acgtggccag cccaccactg aactggtact tgtactcgcc
     1501 gggcaactcg cccccagcgg accagatgcc gcggtagccg tcggctttct cgcagacgac
     1561 gggccgcgat gccggggctg tctcggccgc ttgtgcgaca agggtcattg tcgtacacgc
     1621 cgtcgcgatg caaaaggcac gtcgaaacat tcatgaacct ccgcgcaaag tcgtttcaca
     1681 gatccggata atacctcaaa ggacgccatt ccgcacgtgg tagtgactcg tgcacaagga
     1741 ttcaggaaag gcctgcagct ttcttgacag ttcttccggt gatcttgata atcgcctcgc
     1801 tcttgttggc gaaacgcatg aacactcggt taatcaaaat cgatcgaagt gcagactgcg
     1861 gggcccaatt gcgcgaggcg gcggcatgcc tggccgcggg cggtttggtg gttttcccca
     1921 ctgagacggt ctacggcgtg ggcgccaatg ccgcggatcc ggacgcggtg gcccgcctgc
     1981 gagcggtgaa gcagcgggcc gataccaagc cgttcactgt tcacatcggt tcccgatcgg
     2041 ccgtggaacg ttttgtgccc gggctgagcg ggctcggacg ccggttggcg gagaaagcct
     2101 ggcccgggcc gctaaccctg gtcttccacg tggccgacat cgaggccgca cctgttgtcc
     2161 gagagatttc catggggtgc gcggagaacc tctatcacaa tggtacgatc ggtattcgct
     2221 gccctgatga ccgggtcgca ggtgacctgc tgatggcggt ccggacaccg gttgtggcag
     2281 ccagtgccaa ccccggcggt gaggcggccc cggtcgaagc ggaggagtgc ctccgggccc
     2341 tgggtggaca ggtggacctg gtgctggatg ccggccgaac ccgttacgcc aagccatcca
     2401 cgatcgttcg ggttgatgag aagggttacg agcttcttcg ggagggcgtg ttggacgagc
     2461 gcaccattcg tcggctggcc cacgtgactt ttctgctggt ttgcagcgga aatacatgcc
     2521 gcagccccat ggcagcgggt ctgcttcgga gactgcttgc cgagaagctc ggatgtgcgg
     2581 agactgaact ggaagctcgc gggttcacgg tggaatcggc cggaacgagc gcctgcacgg
     2641 gtgctccccc ctctgtggcg gccgtaaggg cccttgagtt caggggaatc gacatctccg
     2701 gacaccgttc ccggacgctg acttcggatc tggtcagccg agccgattac atctttgcca
     2761 tgactgccag tcatgcgaca atggtcacct cgttgggacc tggtgccgga accttggtca
     2821 gaaccatcga tgaggaagac atcgaagacc ccgtcggtgg caacgatgag gtctacagcc
     2881 aatgcgccga tagactcgag aaggcactga gaaaccggct gaaggagata gctctttgag
     2941 agtggccatc ggatcagacc atcggggata tgcggccaag gagaagatca agtccatgtt
     3001 ggcctccaag ggttacgagg tcttggacat gggcaccgac agtccggcga gttgtgatta
     3061 cccggatatt gcctacccca cctgcaagat ggtgacttcc ggcaaggccg atttgggcat
     3121 tctcttgtgc ggcacgggga tcggaatgtc gatttcggcc aataaagtcc acggtattcg
     3181 tgctgcctgc tgccacgacg aactgactgc cgaaatggcc cggcgacaca acaacgccaa
     3241 cgtgctctgc ttgcccgccg acctcattgg cgaggaactg atgcgtcgga tcgtggatgt
     3301 ctatctcaac accggcttcg agggcggccg gcacgagcgt cggctcaggc gcatcgccga
     3361 gtacgagatg aaggaagggc agaacgtcgg cgaagccatg aagcagggca gtgctgccgc
     3421 tctttcagag cacggcaagg ccactcggaa gatgtaatcg ctttgatcca gacctcgtgg
     3481 gaggcctgtt cccgtgcggg ctctgtgttt cccagttttc gattccgctc ctgaatccag
     3541 tccttgttga cgtcgggccg ccctcttttc tgtaacaaac cctccgcaaa ggtgactaag
     3601 gcagtgtgga ccgatggggt ccaaaggggt ttgataacca taagctcttg taagaaggag
     3661 acttaagatg ctaaggaaaa ccatcaagta tggggtaatc ggactgacgg ggttgctcgt
     3721 gacggccggt gttttgtttg ggaccgatct gggcagttac ctctggactt ccaccaggac
     3781 ggtgcggacg gcagtccgtg actcggtgcc gatcgagttc gagctgcaac gagccagaga
     3841 cctgctggaa gacatcattc ccgagatgca ggccaacgtt ggcctgatcg ccaaggaaga
     3901 ggtcgagata gccaacctca aggaggagat tcagcggggt caagcctctc ttgcagagga
     3961 aaaagcccgg atcacgaagc tcggtgatac gctgaaggca cagcaagtcc gctacgtctt
     4021 cggtggcttg gagtacaccc atgaccaagt caaggcggag ctggctcggg ccttcgatcg
     4081 gtacaaggag gccgaggtag tgctggccgg caagcagcgg ctgctggaca cccgccagaa
     4141 atcgctgcag gctgctatgc agatgctcga gcggacgcgt agcgaaaagg cccgtctgga
     4201 agaccggatt gcggctctgg acagccagca ccggctcctg aaggccgctt cgaccacctc
     4261 gcaggtgcgg gtggacaaca gcaagttggc ccaaaccgag aagctcatag gcgacatcaa
     4321 gaaacgactc gacgtagccg agcgcgttct ggcccatgag gcccagtttg tgcggccgat
     4381 cgagatcgac gtgattagcg agaaggacct cctggcccag gtggaagagc acttcagcgg
     4441 caaaccggcc caggtcgggg cggcagccgc ggaagcgacc agcccgatcg ccatgggaca
     4501 ggccggcgct cgcaccccga ccccatgagt ggggccgctc gcaagcgggt atactggtgg
     4561 tgatcgtcgg caggggttct gggggcagga gtccgatcag tgctgattct caagataaaa
     4621 caggctgcaa ctgcgctgaa ggacggccgg ctagacgagg catacgagct ggctcgggcc
     4681 gaggatttcc ggacccaccg ggaaggccag gacctgatcg gtcaacttgt ccggaagttg
     4741 gttgcccgag ggcaggatca tctctcggcc gagcggcttg gccaggctct ggcggattgc
     4801 gagaaggcgg agcggctggg gggcaatctg cctgagactg ctgcgctgcg tgctgctgtg
     4861 accgatgcga tcgcgaaccg ccagcaggtc gagcgccgac aagccgggct ggtgaccatg
     4921 gcccgtcagc acatccggga cggatggctt tcgcttggcg agcaggtctt ggcggacgtc
     4981 gcaagcgaga ctgccgagac gcttcgcagg gaagctgagg cccgcagggt cagggcagaa
     5041 caagctctga acagagccga gcaggccctg aacaaacagg attgggctct ggcgatcgag
     5101 tctcttcgag aggctcgcca agcccatgcc agcaacccgc aaaccgccga cctgatggac
     5161 cgcgccaccg tgctggttgc cgatcagacc agcaaggcga tcaatcaggg acgattagac
     5221 gctgccgagg ccatgctcga gcacctcgct tcgctgacga agccgacgct tcagatccag
     5281 gaattgaccc ggtgcctggg tgagtgccgc agtgccgcaa gctgcctgga gcggggtcaa
     5341 cctcgtcagg cagccgagat ccttcatcgg ttgtcggcgg cttattctga ggccgcctgg
     5401 ctgaccgagg catgtcgaca agcagagcaa gcagcggcgg cgactgagaa cctgcgacgg
     5461 gggcccttgg gctggttcac ggggagcggt ccgcccgtcc ggactcccgt gccgacctat
     5521 gaggcggacc gcaccgacac tatgccgccc gccaagccgg cgaagggaca cgccgcaggt
     5581 ggcgtagtcc ctgatcgttt catgctgcag atcgacaccg tgggtggttt cctggtcctg
     5641 cgacagccgc gagtcaccgt tgggccgatc agctcatcgc ggcggccgga tctggccttg
     5701 ctggccgagg ccggattgcc tgtggtgacg attgaacggc aggatgatga ttacttcttc
     5761 tcgtcggaga cgccggttcg cgtcaacgat atgccggtgc agcgaaagct gctgtcggat
     5821 ggcgatcaga tttcgctcgg gccgagatgc gtcgtgaagt tcacgctgcc cagtgcggcc
     5881 agtacctcgg cggtgctgca cctgtcgggt tgccggttgc ccagcggcga cgctcgacga
     5941 gtcattctca tggacaagtc gatcgtgctt ggccccgggt ccctggctca tatccgggcc
     6001 gaccagatgg atgcgcccgc cgtcctgcat attcgggatg aacggcttct ggtgcgaagc
     6061 acgaccgtgg taaagatcga cggccggccg atcgacgatc gggtcggcat tcctgtcggt
     6121 gcatgtgctt gtatcggacc gatttccttg gcggtcgcga gggcagaggt cagagcatag
     6181 aaccgcgcgg cggtaccggc atcgaccggt tgtgttccgc tgaagggaag ccacgacaat
     6241 ggacggattc caatacaagt ttggcgacca gcctttggag ggctacacca tccagcgggc
     6301 catcggtcgc ggcggtttcg gcgaggtgta ctatgccgtc agtgacagcg gccgcgaggt
     6361 ggctctcaag gtcgtccagg gctatgagga catcgagctg cgcggcgtga gccagtgcat
     6421 gaacctcaaa agcccgcatc tggtcaccgt tttcgatgtg aagcggaatg ccgacggacg
     6481 gcccttcgtg atcatggaat acgtggccgg tccgtcgctg cgagaactgc ttcaggaggc
     6541 cccgagtggc cttgggattc agaaggccgc ctttttcctt cgcgaaatcg ccaagggatt
     6601 aacctatctg catgatcggg gaattgttca ccgcgacctt aagcccggaa acattttcta
     6661 cgaggacggc tacgtcaaaa tcggcgatta cggcctcagc aagatgatcg cttccagccg
     6721 gcattcggtt cagacgatca ccgtcggcac cgtccattac atggcccccg agattggtca
     6781 gggcaactac gaccggagca ttgacattta tgccctgggc gtcgtcctgt acgagatgct
     6841 caccggccag gttcccttct tcggttccag ccacggcgag gtgctgatga agcacctcat
     6901 ggctcagcct gatctcagcc agatcgagga gccgttcgcg tcggtgatca ggaaggccat
     6961 ggccaaggag ccggcggagc gattccagtc ggttcaggag atggtcgaag ccgtctttgg
     7021 cgccgagcac attcagcaga gcgtatcgca ctttcggccc gagagtcttt cgatggtcgc
     7081 gcgccgcgtc gcccagaagg tgaccgtcgg cgggtcgggt tcatcggccg agcatgccca
     7141 gggcttggga cccactgcgc cgagtgggga ggccggcagc agcgacgtct ggcaggacct
     7201 cagcaagcac atcgatgatt tcggcgagaa ggtaggcaag tggggcgagc gtttgagcga
     7261 tcggatggcc gcggtcggcg accgggtcgg ccggcagttc ggccagaaac cggcagcact
     7321 tccctatgcc aacgcggccg ccccggcttc ggacggcgtg atcgatacgg cactggatcc
     7381 actcgaccgc cgggcaagga aacgcttgtg ggtggtcgct gcgctcgtca tgggcgccgg
     7441 aaccgggatc atcgaggcaa cctcgcacaa cggaggcccc gcgatagaac tcagcatctt
     7501 cgccaccgtt gcgatattgc tggcgtctct aggcatcttc cgcgccgggc gcctgctggc
     7561 cgagcggatg cagaaagagc gcgggctggg tcgttacgtg gccttcggtg gcgctgccgc
     7621 cctgggagca ctcgcggccg tattcttcgg agaaatcggc ccctttcgca agcagatcca
     7681 cgcagccctt gacgacaacg cgctgccctt gctggcatcc tgcttcttca tcgactggtt
     7741 gggtcgcatg aatcccgtgc gtcaacagcg catctcgttc gagagcgcct ttactgccgg
     7801 gttgctcggc ttcatggtca gcattttcac cgacgccagc gcgatcgtga tgatcggcgt
     7861 cctggccggg ctgtcgatgg ccgtccaggc gggcagcgcg ttcatcccac cggctctccg
     7921 caaacagctt gggataacaa gaggcaacgg ccagcacgat catcccagac cgcccgagag
     7981 aggcacacct ccggcggccg accgaacccc gcagatccag aacgctgaag ctcatcgccc
     8041 tcgcccgcta cccgttgccg tgcctcctgg aatagaagta ccgttgtttg tcagggcctt
     8101 gtcgctgctg gcgttcgccg ttctcctcgg tggcgggatc attttcctca tcctcgcggg
     8161 catggcgagc ccatccaatc tcgactactt tgcgggctgc gtttccgcag gcatcggggc
     8221 tctgctgttc agcgtctttg ctttggtaaa ggccacgcaa cggtacctgg tgagttggtg
     8281 gagcggtctg ataaaaccgg ttctgatgat cgccttgctc gtgacgggca tcgcctcggc
     8341 agtagcactc ggctcgatgc agcttcacaa cgacgacgag atgatcgcga tagcggtgat
     8401 cgtgttcgcg gcggtctttt tcatcttcgt cgcctgcaca accaaccgca tgattcacgc
     8461 ggtcctgggc cagccctttc ctgacgccgg acgggcaacc ccccgagccg agcatgccgc
     8521 cggcgtctcg ccgcgaggcc gtctgtgggc catgctgttg gccttgctgt tcttcgcgtg
     8581 ccccattggc ggactgcacc ggttctacgt cggcaagatc gggactggcg ttttgtggct
     8641 ggtcacgttc ggcgggttct tcatcggcca gattgtcgac atcatcatga tcgcggccgg
     8701 tcagttcacg gacaaacaag gccgtccgct gttgacgtgg accaacccca acgagttcaa
     8761 gggccgcccg acggtcgcta ccgaaatctc acccccgcct gcttctgcgg tcgctacccc
     8821 catggttgcg cccggcgacg tccgtcccct ggaactcgga agcctgatgc tctccggcgt
     8881 gggcatgctg gtgctgctgg cgggtgtgct gacaggcctg ctggttgccg ttcatctgcc
     8941 ggccatcatt gcagcgggta ttccggatca gagcttggag caggaactga cccgggagtt
     9001 tggttattcg aactggccgc agctgcttga gcgaatcggc accttcgtgg ccgtattcct
     9061 gtttctagcc agttgtgcaa tactcctgct cgctcgccgc aagtccggag gtgcccactg
     9121 cctgcgtgcg gtgctggggg ccgtggccat cggtgtgagc ctggctgctt tcgccgacgc
     9181 aaccagccgg atccaatggg cgacagtatc gaccctgttg caggccgagc ggatcggccc
     9241 ggccatcgac gcggcactcg gcgcgatcga taaggaaggg gccactatct ccgcggtatt
     9301 gcttctgatt tcgattgtcc tgctgttttg gccagccgat cgccggcagg cggatgtcac
     9361 aactgtcggc ggcgatgctg tgtcggcgcg gaggtgatgc catgacacac gtggaggcac
     9421 tgccagtggg aatcagctgc tttggaatct tcttactcat cgccttggcc ggccttgtgg
     9481 ttttgatcct tggaaaccgc ggtgctcggc gcgtgcttgc cggtctggca atcggcgttg
     9541 cggtggtggt cgtcttcggg ttgttggcga tgttttgggg gaggagcagt gtccattaca
     9601 gcacgtcatc gccgacactg ttctctgagg ctgtgtaccc gcccgaagat gaggcgatgg
     9661 agagccacgc cgtccgaggg gtcactatcg gcagcaacgg cgttgtcgta agagagggca
     9721 gcagttcgat cgttgtcaat ggcaacggcg tctcagtgac cgacgagtgg attgacgccg
     9781 ggcaggcgga tttcgaggct gacgtttacc cctcggagcg atccgcggcc gtagcgctgg
     9841 cccggaaagc gatcaagtcc tgggatcggg ttctgccagg tggacaggaa ccgacccaga
     9901 ttctgattcg cggtgacagt gagttggagc ccgctgttgt gccggcagtc ttgcaagctt
     9961 tgcgggcgaa gctcggcgat gatcgcgtcg aaatggataa gtcgccacaa gaagcacaga
    10021 tcgaagaagt tgagcgagtc ttggtcagtc cgggctcagc tccgtccctg ccggaggccg
    10081 atgcgacggt tcggctggcg atcaacggga acttcagcta cgttcatggt ggtcgaggcg
    10141 tgattccatc gcaaaagaga aacgggacca ttgagctggt tctctcgggc ccggcgggca
    10201 agagcacgca caccgcagcc ttcgtcaaca agccctgggt ggagtacgga atcgaccggg
    10261 ccgcgcaagt atccggccgg cagccgatgg tcgcgcgttc gaagcggctg tgcccgacac
    10321 gcggcgaggc cgaacagcag gctattgccg aggccgcgaa gaacctggtt ccgctcgtcc
    10381 gcaccgccct ggaacgtctg gagagcagcg gggccagcca gtcaaaccgc ggcgcgattg
    10441 cgatacagca gcgccggccc gatcctgagc tttacgagaa gatccgagcc gagttactgc
    10501 gcgggaccgg cgtcgacgac cagttcacgc agcatattca tcgaccttat ggcgatgtct
    10561 ggcagcacgc tctgctggtt gatggatcgg atgggcaaat caccgcctat gctcaaacgt
    10621 ttctccccca gatcgttcag caacagaaga ccgccctgcg gatccgcaag tcgtgggcgc
    10681 ggacgatcgt gggtcttgcg ctgttgactg tcctgatcgc ggttgtgtat gcgtttctga
    10741 acgcggcaac cagggggtat tacacatggt tactgcgcgg tagcatggtg ctgctggtgc
    10801 tgggtggggc agtagtgctg ctgctcatga cctgaaggcg gcgggtgagt cccgcgtagg
    10861 gcacggtcgg agccgccgcc ggcggcgcag acctgccgct gcgtaactgg ccaaagaaaa
    10921 cggcaggtct cggtcgctgc ggcgacctcg acactgccct acagtccgcc tctgcggctt
    10981 cctggaggaa acagacgaca gcgtggcgaa cattttaacc gaggccgaac agtacctgct
    11041 cgatcagatc cggcagggtc gaccgcaggc ctgggagcag ttcgtggacc gctaccaggg
    11101 ccggctggag gcctttgcgc gcagcaagct gcgcaccccg gccgacgccg aggacctcgt
    11161 tcaggacgcg ttcatgagtt tcgtccagtc gctgggggct tttcgcggac aggcgagcct
    11221 cgaaacgtat ctcttcgcca ttcttcgtag aaagatcatc aactggggcc gcggccgcaa
    11281 atcgagcatt tgcctgcttc aggatgcgct gcccggcggg gggcatgatg actcgggcga
    11341 cgccgccgac cggttggccg ctcccgatca gacggccagt tggtacgtcc gtcgcgatga
    11401 ggagcaggag cggcggcacg aggccctggc ccaagccatg caggaactgg tcgacggctt
    11461 caagcaatcg ctcaactttc gcgatttgaa gatcaccgaa atgctcttct actgccgatt
    11521 acgcaacaag gatatcgcca acatcgccgg ggtccgagag aatcacgtgg ccgtgaccaa
    11581 gcaccgctac ttgaagcagc ttgccgagcg gatgaccgcg gtcctcggca attcgtcggc
    11641 ggcctgtgac gattcatttc tgactgccga aggctccgat gccatgcttt gcgagatctg
    11701 gcaggaacgc cggctcagtt gcctgaagcg cagcaccatt ggggcttacc tcctcggcac
    11761 gctggagccg gagtggcagg actatgtcac cttccacctg gagcggcttg gctgccagtt
    11821 ctgccgggcg aaccttgatg atcttcgccg acagaccgcc gacgacggta cgcgccgcct
    11881 ccgcgaccgg atcatggaat cgaccgtagg tttcctgcgg aagccgtgag gcctattgtc
    11941 ggcccaccct gttcagtcgg gcaacccggt ccttcgatgg cggcgggaaa ccaggacata
    12001 caaaatcgca attccggcca gcaggatcag cgcaatcagc agctctcgga tctgggcgtg
    12061 gctgataagc accacgcaga tgatcgcccc gacgaccggc acgaagaggg gcacctcgaa
    12121 ggtacccttg ggttcgcctt gtcgacgctt gagcacgaca agtgccagat tgatgatcgt
    12181 gaagacgccc aacagcagca cggatgttgc cttggccagg acggagatgt cgccggccat
    12241 tgccagggca atcacgatga gcatcagtat caggatggcc gagtcgggtg tgcgtcgctt
    12301 gaagtggact cgaccaagga cggcgggcag caggccctga tgagccatgc cgtaaaccag
    12361 ccgcgacccc atgatatagt tcaacagcgc ggtgttcgtg atggcaaaca ggcatacgcc
    12421 ggtgaacagg accggcggac accagggcgc cgcctggcga accacttcca caagcgggcc
    12481 gccggattca gcgagttccg cgtgcggcac gaccgagatg gccgtgatac tcaccgccgt
    12541 atacaggccc gctacgacga acagggccgt caccagagca cgcggcaggt tcctctcaac
    12601 gtctttgacc tcctccgaca cgttgagcaa atcctcgaag cccacgaacg catagaaagt
    12661 caacactgct ccttgcagaa ccagcgatgc tggtccgacg gtcgcgtgcg gcggcatttc
    12721 caggtagttg acgcctcccc agtaccgcaa cccgacgatc acgatgaaga ccaggccgcc
    12781 gccctcgatg gtcgtacaca ggatgtttac ccacgtgctc tcctgcatgc ctcgaaggtt
    12841 gatgatggtc agaagaagga tgaatcccag gatgatcagc ggggccggca gggggccgct
    12901 cagctccatg aaatagccgg cgaacgcccg ggaagcagtg gccatagagg taagcccgga
    12961 cgcaaccacc gcgagcccga tgagatagga cagcatcggc cggccgaaag ctcgttgggc
    13021 gatgtatgcc gctccggccg atcgcggata gcgggaaccg aggcaggcgt acgagagccc
    13081 ggtaaacatg gccgcgaaca tgctggcgag aaacgccagc catatcgcgt tgcccatgac
    13141 gcccgcggct ttgccgatca gggcataaat gccggagccc agcatatcgc cgacgccgta
    13201 aatcgccagc gaccagaatc ccatggtgcg caacagcgca tgctcttcgc gggcaagtgc
    13261 aggcttttca cgcggagggg aagatacatc tgtcatgggg acattgtaga agccaagtcc
    13321 gccggcgcat agctgaccgg gtcgccccgc atgatgaaac ggtcttgggg ggcatccagc
    13381 cttctggctc gtcggccgat cggcaagatg tcaggtttca catcgaagct tcagtatgcg
    13441 atttcactcg taggcccgta tctcaaacac cctggcggat gcgtcgccgt tcgtcgcgtt
    13501 gatgttgagt cgcaaaccac ttgcggttac cgggtcaaag cgatggagct tcttgcggag
    13561 gtaattgccc gtcacctggg cgactgtctt ggccccgcct ttatcgagaa tctgcagctc
    13621 atagtcgcga acggtttcag gctgggcatg gcgaatcatc ttggcattct ggtggtcgct
    13681 gagggtcagt gtcatgcttc ggttcagagc ggtgtcaaac gtcaggtgca cttcgcggat
    13741 ccgggccggc ttacggaacc gcagctcgat ccactggggc atcttctgct gcgggtcgga
    13801 aatccatcga tgcgtcgccg gtccgacgcc gcgggtgacg ccgtcgatga cgttcgcggc
    13861 cctgccttgc acagtctcgc tggatgctcg gatctcggcg gttcgagcca gatcgagcga
    13921 atcggagttt gtggccccga tgatgtaggc atcgtccttc agaagctgct gctgcaaatc
    13981 gtggatggcc tgcctgccga gctcacgggg tgtgcacttg tttcggatgc acatgaccgc
    14041 cgccgtgccg actgcctgcc ccatgaccga accggttgcc attacgcggg tgctgccgaa
    14101 ggccacgtga ctcatgcttg cgtcccggcc cgcgaagaac aggttctcga tgttccgtga
    14161 atacaggcag cgcagaggga tattatacat cggcacgcgg atttgcaggc acggtttctc
    14221 cttgctgtga atcccaatcg acgggtgcaa gtcgatcggc cacccgccgt aggccgcgcc
    14281 gtcctcgaat tgttcaccac gctgcacttc ctgctggatc agcacatggt cgccgacgaa
    14341 tcgccggctc tcgcgcttgc cagggatgaa tccgaaccaa tccagcgccc aattggcgct
    14401 ttcggggtgt ttgccgctgt tcttgatgtg gtcccagacg cccagggccg ccgcgatcaa
    14461 ctcatctcga attctctcgt tatcacggat ggtgtcgata tggcctcccc attcgaccca
    14521 ccagaagccc cattcccaac taccgtgcga acggtaaggc agatcctcgc atgtcgggaa
    14581 acgcagtgcc cagtcaggcg gcgtaaaggg gatcggctga tcgtatcggc gggtggtgaa
    14641 caggatcgaa ctgccgagca tctggttgtc tggttgcggt ggagccaaat cttcgccgaa
    14701 ttccgtgcga ccctcccgtc cggtccggaa ttcggcgccg gcctcagccc ccagtcgccc
    14761 atcgccgctg gtgtcgatga acatcttgcc gctgatcgta aacacgtcct gcgtgccggg
    14821 tctcgcggcc agcacggccg cgatgcgcga tttggaagcc atcaccgcgc cgcagcatga
    14881 ggtgttcagc aggacagtaa gcccctgccg gcggcagacc atttcgtaaa ggagaagatc
    14941 aaatactcca taggaacggt gcggattacg gacgacctcg tggaggcgaa tctcctcgat
    15001 gataccgctt tcgcgggagt cggtatccga gcgggccccg tgctcgtcgg ctcccagaat
    15061 gtggagccgg atttcactcg aggcgttgcc cccgagtact ggccgatcgt gtaccagcac
    15121 gaccgaggcc ccgtggttca aagctgccag tgctgccgac acgcctgcca tgccaccccc
    15181 gacaatgacc acgtctgcct cgatcttgtt cgatcgggtc aggcggcttc cagactcaaa
    15241 gacagcggcg gcatcggctt gcccggcttg ccccaggccg ctgctttcag ccctgcaccc
    15301 acccaggagc ggggtcagcg ccgcgccgct tccagcggcc aggaacaggt cccgacgtgt
    15361 cagatgccgg taatcttgct gatcctgtgt catctcaacg ctcctctcga tgcggatgtg
    15421 acggatttgc cctcggcact gcccggggcc ggtttgggag tatgaggcta gttgcccttc
    15481 ggcgtcaacg tccacggctt gttgacgtcc cgacggcgga gggtagcatc acttctgggg
    15541 atggcttggc gggcgtgaaa gccggctcca cgtgtgttcc cggccacgca gccatcccgg
    15601 ccgtaggcgg caatgccgat acagcgcttc tcatgcagca ttctcccctg cgtggcagaa
    15661 ggtgagcgga atgatgacgc atggacgatt ggtgggtgac ggatttgtgc agacggggcg
    15721 gggtctggcg gttctggcga tgggcatgat gctcctggcc ggttgtgccg gccgggaaca
    15781 ctacgaccgc ggtcgtatgc tgatggcttc cggtcagtat gaccaggcag tcacggcctt
    15841 tgatgaggcg ttggctcagt ctcccggcaa cagcaggtac cataacggcc tgctcgaggc
    15901 caagtctttg gctgccgacc agcacgtcaa aacggccaca cagttcgttg ccgagaaacg
    15961 tctcagcgac gcacaaaagg agctggatac tgctttgaag ctggtacccg cgcacccgga
    16021 gggcgtgcct ctggcggcgc aaattgcccc ccagattgcc gaatgcgaga agaccattgc
    16081 cgaggctcgc gaggcgctgg cccgccagga atggaccgag ggtgcgaggc tgatcgccga
    16141 ggccgggaga atcgaccgga gtcatccaca acttgaactg ttgcggaagg aggctgccga
    16201 tacggtcacg gctcgccagt tgttggccgc ccggcgagcc ttgggcgcgc gtgactggga
    16261 tgcggcgctg gcggcctgcg cgcaggtccg gcgagtagcc cctgacaata ccgaagtcgt
    16321 tggaatcgaa cagcaggtga aagaccggcg agaggcacag cgggtgtttg actcggccaa
    16381 ggcgatgatt gacgcaggga actcgagcgg cgcgctcggt ccgttgcagc aggcggcggg
    16441 gctctggccg gacaacgacg agatagtatc gacgcttgac cgggtcaaag ggcaagtggt
    16501 cgacaagttg tcgacccggt cgcgggacga agctcgggcc ggccgtttcc aggcggctct
    16561 tcaaacgctg gacgaagcca tggtcctgga gccaaaccgc gagagcctgc ggcagcagcg
    16621 aaagcaggtg ctcgatggct ggatcgctgg actcatggaa gactatcgcc gccacaaggc
    16681 ccagggagca tgggagttag cctgggtcga cgccgtgcag gctttggcct tggccccgac
    16741 acagcccgaa ccggtcgtcc aggcttgtct gacggccgag gagggaatcc ggcgagctat
    16801 cgcatacaac ctcaacgtgc tgctggcacc atcagacgaa cgccgcctgg acgacatcat
    16861 ggccgtctgt accgtgcttc tcgaggaact cggtgaccag cgtccggatc acgtttgcct
    16921 gacggaacaa tcgatattgt cgaagctgct ggcggaattc gccgtttcat cggccgatgt
    16981 gaacaatcgc gacaagctcc aggccgtggg ccggcggctg cgaggtccca acgtatttct
    17041 cttcgtcaat atggacatcg tcaaggtaaa cgtcggcaac ccggagtcgg cagcatcggg
    17101 tacgtcccga ctggatctcc ggatgaacat ggtcgacgta tcgagcggca ggttactttg
    17161 gaaatgtgtg gagatgcttc cattggaagg atctgcctcg ccaggcggta ccgctgcgac
    17221 cgcgaggccc gtcgcggtgg tgcatgcgtc agggccgttg acggcggacg gtatcgcggt
    17281 gcttcgaccg gtgttgcggg ccagagtgtc ggacatgtac cggggacatg ccggatcttt
    17341 tctccaggcg gccggcgccg ccaccgggga cacggcgacc gagttcaacg ttcgattcct
    17401 tttcgacctt gtgtccatgc ccgacgcgca gactctgggg tcggttctgg atgccgtctt
    17461 cgggtcggtg gtgagcgggg acatattggc cgcctgcaag aagatcgcaa gtgaacggct
    17521 tgccctcgcc aagcgagtgg cggcccggga cgagcatccg gctgagaaga tgtccggccc
    17581 ggcgattctg cctgcatcac ggccggcggt ctcgaccgcc acggcacccg tcgcccacca
    17641 ggcaccggcg gttccttccg cgtcaaaacc cgcgacagcc gcgcccgcca gtgcagatgt
    17701 ccggtccttt cctgcgactc agccccggcc ggtgcccgtg ccggcttcga cgtcagcggc
    17761 ttcgacggcc ccggccgaag accgaggccc ggttcgcgtc tttcagggcg tcgtcagtcg
    17821 cgacgacgac cggttcacca aggaactggc aacggtcgat gggatcgtag tcaagcttct
    17881 ggataccgac gcagacccgc ttgatgccga tattgagatt cgcgtgggca agcggagcaa
    17941 gaagtatgag gacaagcccc aaggagcccg gatcggcggt tacggtgagt caggtcgtgc
    18001 gtatgtggtc gtgattctca agatcgatga cgagaccgag accgtccatt ttgccgttga
    18061 gcgggtggac accgttcctc agcaacagcc ataatcgatt actttcccgt cagcacaaac
    18121 ctggccaggg tcggttgtcg gccgatgatg tgagtgatgg tatagttgag caggtcgaaa
    18181 gcatctcgcg ctgtcgccgg cgtcagtctg gcttcgcgaa gggcatccag tgttgcgccg
    18241 gacaccttgc gtttgtccac gatgcccggt tcgcaattac ggcacgccaa gccgccttgg
    18301 tggacggcca agaaacccgc ccgtccggca ggggccggct tgccgcacat gacgcatcgg
    18361 ctcaggtccg gccaaagacc cgccgaacgc agtaacgcgc attggtagcc gacgagaagc
    18421 ggcagcggct cggccccgca ggccagcccc ccgagcaaat cgcggagagc gtcaaaaaga
    18481 tcggcatccg ggtctgcctc ttccaccatc gcggttgtga tctcggccgc gtactgcccc
    18541 ccgtatagcc tctgaaggtc ctctcggagt cccatataag catcgagttg ctgccattcg
    18601 gtcagtgtgc caagtgaggc gtcagactgg aacttggcga tgaatgccac ttgcccgcgt
    18661 tcaaggaggt cgatgccggt cgcaaaacgc ttgctggtgc cccgcttgat gccttttgcg
    18721 atcagcctgc ggcatccgtg ctcgcgggtc aggaaaacga gcacttgaga ggtctcggag
    18781 taatccagac gcttcagggt gatggcctca tccttgatcg ccggcatgaa cagatccctc
    18841 gcgcatctcc cgatccaaca tgagattacc atatcagccg ggccatagac ggacaatctg
    18901 tcttcacatc gccttgatat cgaggacttg aagtgcggca tggcgctggt tgccaatgcg
    18961 gcacgccaca gcccgagatg aatcacctct gtgttcgcga cgggcctgct gggtacacgg
    19021 caatagcgat gccccgcccc tcgttctgcg ggaggcgggg catctgaagt catcgcggcc
    19081 ggtctattct gcttccgcgg aggccgggcg tcaaagcccg ccgaggctct atctggtcgt
    19141 gggcatcgga acgatcccgc tgcgaggccc gaacaaccgc actccgattc cgatccggtc
    19201 tgcgtaacct tcctggccga gcagcagttc aaggcggatg atctcgcctt tttgcgtgac
    19261 acactgccgg tggcctggct ccggtccccg ctcaccgatg atcaatcgga atgtgtatcg
    19321 ttgaccgatg gccactccga cgttgctggg caagacagcg aacagccctc cgtcgcctat
    19381 gttgaggcat tccgctgtga ggggcggctg tccgttgtcc tccgcgtcgt tgaacagttg
    19441 cacgctgcaa cgtagggtgc ggcgacgtcc ctgccgcttc tctttttcgc ctgtggcgat
    19501 ttcccagcga cggaggaaag tgcctgaatt gctcgccaca gggaaccgat ctgacggacg
    19561 gtggtaaatg tgaccacctg gttcccgaat ccctggccga accccagcta tcgatgacac
    19621 cggagcctcc ttgctccttt ccccatttcc gaaaccaagt actgcgacgt cccggcgacc
    19681 gcgattctgc acgccggcgg gctgacctgt acctgcgggc aacgaatcgt tcccgcacct
    19741 ccatgcctcg gcttgccgtg tccctgagac tgcccgcaag agatccagcg atctcgggca
    19801 gtgattactc gatcggtatc tggcacgtgc tctaggacgg tgtgggatcg gccggggcgg
    19861 cagctactgc gtccgacctg cattcaacgc gcgtcgattc gcagggcacc cgaaagaata
    19921 aagccaaaac acaaatctcg gcacttccgc cctcatgagg gcccacgtcc cgagctcgtc
    19981 ttttcctgaa aatgacgcct taattatact gcaaagcaaa cccgaagcaa ggcgcgtttg
    20041 gccaattgct caactcaacg gcttgttttt ccgggacttc aagacggccg ggcgaacgcc
    20101 tcgccgggct tctccgccat tggtcgtctg gctgggtttg gctggttcac aaacccgcct
    20161 gcggcggggt gtttgcctga tttccggacg agccggcgtt tgcgggttga caggtcggat
    20221 tgcacaacgt ctaatagtat tggccgcaga tgtcgtaatg acatatagcg gctgcggccg
    20281 cgaatacgtt atatggtttg caccgcttga gcctaccgcc atcatccata catttgccct
    20341 caagcgggtg gtgcataccg atttgcggcc aaccatggca cagcgggtgt cgaatgggcg
    20401 gtcttatcgc cctgaaacct cgcggggaaa agaagtcgca cggttatcga cagacggatg
    20461 aaaccatcgc gccgacacgg cgagagggtc cgtccaacga tgagaagacg gtgcgaccgt
    20521 cggcaaacgg tgccgaatgt gtcgcgggaa tagttgggtt tcagtcgtcg gctgtagcct
    20581 cggtcggcga cggtgctgga ggcggcgttc gaagaagact gcggttcggc ggccagacgg
    20641 cggagattgt cggctcgtat gcggtgtgac ccgcaaggcc ggttctcatt ccggtaattc
    20701 catacgcttc gctccgccgg acagttcgca gcttcaggac tcttcagaca aagccgaagg
    20761 cacgaaacat gtgcgtaagc acaggaggtg tctcatgagg cgtaagagat cgggctttac
    20821 cctgatcgaa ctgctggttg tggtcgccat catcgccctg ctgattgcga tcctgcttcc
    20881 cagcttggcc cgggcaagag aattggccaa gcgaaccgcc tgcgcggcga acgtgagcgg
    20941 catgggcaag ggcctgtata cctatgccac cgaaaacgcc gacgcgtacc ccctcgcggc
    21001 cccgaacact tggacgcacg ggaaccccaa gacggccgtc acgtactgga acatgaccgg
    21061 taatcggggt ggtccaagcg ccggtaccgg cattacccca gccaataccg gtgatccagc
    21121 cgctgcgaac ccactgatga gtcagctgtc gacgacccgg aacctgtggc agttggtcaa
    21181 gaccggcgga acctcgccca ggtcgtttat ctgcccgtcg agcagcgaca acgccgatac
    21241 gatcgataat cccgctgact actgggattt tccggccaag gctggcgatg gaaccgtgaa
    21301 tcgcggatat gtaagcggtc aggataatga ggggtgcatc agttacggtt atcaggttcc
    21361 ctacggagat cagggaaggc cgagcaccga agtcgaccaa cgcatggcgc tggttgctga
    21421 caaaggacca tactgtttca aggtgaatgg ctcacccccg cctaccagtt ggaacatcac
    21481 ggaggcacag aaccagagcc ccgatatgtg gatgccgtat aattctccca atcacggcgg
    21541 acagggtgcc ggcgaaggcc aagttgtgct ttatgcggat agccatgccg agtttcagaa
    21601 taagcctatc gtcggtgtgg gttttgacaa catctacact gcgtggccat ccagtgggaa
    21661 tctgcttgct gacgcgaacc tgcgtaccgc tggagtcaag ccggcgagca acaatgctca
    21721 gctcgtgccc cgtgtgaata ccgactcgct tatttacccg taaggtcggt tatggcagcg
    21781 ccattgcgat acaagcgatg gcacacggaa gacgcaaatc cggcaggcca gcctcggctg
    21841 acctgccggc tctttttacc ccacgacggg tacccaagtc gtgggttggc ccgtgcgcgg
    21901 ctagcccgga gccccaactg atcgaccggc ttggcttgcc catgggttgc cctgcctccg
    21961 gctcacccgg caggtcgact gctttgggtt accgtcacat gagggaccgt ttcacttgac
    22021 attgcgtccg aatgccgtgc gagcgcagct tcctgtttgc cccagggcac cgagaagggc
    22081 gcccagatct gagcacacct tgttgtagaa gatggcaacg tcgcgctgcc ggcggtcatt
    22141 agctgggatg aaacggtgga gaaggcaggc cctgtcggtt accgccctcg gaaatgcgac
    22201 gggaggtcgg atggctagaa ttagccataa tcagtttcga attaaatctt ttcttgactc
    22261 tgagcttttc ccagcgggat aatctaagcg aggataaccg atcgggggcg tcttgggtgg
    22321 tcaggtgtac accccattca ccagatggct gcagtggttg atctcttgag tggcatagcc
    22381 acgggagagt tcgccgagcc gcccacgcga tggacacacg cgtgcagcgt tcgttcatcg
    22441 ggtctgcaaa cccaggttgc aagccccact tcgattggta ccggaagtgt taggtcgggt
    22501 ggcccacctt gtgtgacgtg ctcgaatccg gcagccgggc aacaaggcct tgggttttgc
    22561 caaagaagcg gagatgtgct atctcgcccc cgaccgtcca gatatgcgcc tggtcgcacc
    22621 ccacacggat gggcaatccc cccttgccct tctacccgcg accaggccct ttttttgtcc
    22681 aacctctgat gtcccagccc cggccatgaa tgggctgcca agtccgccac accacagacc
    22741 ggtccgcttc cttgagttcg cctcggtgaa cccatctcac ggttcacaag gaatccgccc
    22801 taaggcggac cgcctttttg cgggccgcaa cccaagtgct ctgcgatcca gaaacatgcg
    22861 cgcaagccgc acagatgtct ggccgtaagg ctccaaaagg cacggtggtc ctcacggact
    22921 ctgtcggggt ctcgaaaacg gtcgccgtgc caccgcctct ggcaaccgcg cgtgatgtgg
    22981 tagaattgag ctggcggagc gggcgaggcg gtcgctcgat caacgttgcc gcgtcgatcg
    23041 ggaggaaagt ccgaactggg cagggcacgg tggttgctaa cggcaacccg gcgcgagccg
    23101 agggaaagtg ccacagaaaa cataccgccc ttgacatgcg gcccgggctt cgcctgccgg
    23161 cgcgagccgg agacgcgaag cgtccgccgc atgtcaaggg taagggtgaa aaggtgcggt
    23221 aagagcgcac cgcgttgcgg gtgactgcga cggcagggca aaccccaccg ccagcaagcc
    23281 ccaataggca gcgagaggcg gcccgtctcg tttgagctgt gggtagggcg cttgagcggc
    23341 ttggtgacag gccgcccaga caaatgatcg ccgctcttga cgcgccgtgt ctcgcttcgc
    23401 gagactggcc gtgcagcggc cgcaggcgaa gcctgaatcg gcgtgtcaag ggtaacagaa
    23461 ttcggcttat tcgcccgctc cgccgattgt tggcagatct gttgattcat agacgggagt
    23521 ggtctctcgt gtcctattca tcctccgtca tcttcgatca ttcaaaggag agatcgcatg
    23581 gtactacatg tgcttcgagt cattttcgtg gctctggtgg ccattgtggc gatgagagtc
    23641 gcggagtact gctggcgggt catgcctgcg tacgccattc tttcgtttgc cctgggattg
    23701 gcctgcctgg tgatcgccct cgacatcttc attccgcaga aatcgttgct ggccatttcg
    23761 ggattgttct tcgggcttgt ggtgggcatg gtggtggcgt tcgggttggg cttgatcctc
    23821 gacgcagtcg tgctgcccta caagtccacg ttaaccaaag tgctggagag tgacgccatc
    23881 gggggcttct ggccttcggc gccgattccc gaaggtagtt atccggagga ggacactgcc
    23941 gctcggcgtt cgggttggga ctcagacggc ctatccggct ccgaaggagg catgttccgg
    24001 aagctattgt gggccgtgct tggtccccat cgaggcggat ctgcccagcg gcctgccacg
    24061 gccgtccagg tcgagcgaca gtccgtcggc gaggcgcgat tcaaggagat gatgtcctcc
    24121 atcaaggtgc tcgtcggtgt ggtgtgttgt tatctcgcgg taagtttcat ccttcaaacg
    24181 aaggacgaca ttcgcttcgt gatcccctac gtcgagttcg tcaaacagcg aaagggcgag
    24241 catccgttga tcctggatac gtcggtgatc attgacgggc ggatcattga cattctcaag
    24301 gcctggcatg tggactcgcc ggtgatcatc ccacgtttcg tgctccagga gctgcacgcg
    24361 gttgccgaca gcggcgacaa gctcaaacgc aaccgcggcc ggcggggcct tgacgtcgtg
    24421 aataagctac aggagctgga aaccatcgat cttcaattcc aggaagcgag gacgacgcgc
    24481 gaggaagagc gcgagggtgt cgaccatcgc ctcgttttcc tggctcgcaa gatggaggga
    24541 cggctggtca cgaacgatta caatcttaac aagctcgccc agatccgggg cgtcgatgtg
    24601 atcaacatca acgatctggc caacgcgctc aagcccgttt ttctgccggg cgagaccatg
    24661 caggtgaaga ttatcaagcc cggcgaagag gttggccagg gtgtcggtta tttggaagac
    24721 gggaccatga tcgtggtcga gggcgcccgt gacaagatcg gccagtccgt gaccatcagc
    24781 gtgaccagcg tcctccagac cagcgccggt cgtatggtct tcggccgaat gggtgatgct
    24841 ccgccgcccc ccgatcgttc gcgccgccgt cagagtcctc ccaatcggcc atcgacctga
    24901 tcagcctttc gtcacaagaa ttcctggatg ctacttgccc ccgaagtcgc gctcgtaccg
    24961 ggccagcagg gtgggaacat cgttggggat gatatagtac ttgccgtgcc gaatcttggt
    25021 ctggcggggt ttcaggccgc caatacgaac gtcgctatgg atgcaaacga taattccctg
    25081 gaacagttcc tggacgtggt cccaggccat cgcgagaatc atcttttcat ccgcggagaa
    25141 gcagccaacc agaccattga ccggccgggt tctgctcagc gggcgagggt tgacgtcgtt
    25201 caggtcgacc ccacccggca catagacctg tccgggcgta tagcgggctt tggtctcacg
    25261 atgcgtctgg tccatccggg tcaggccctg gtcggtgaag atgaagcact tctggaagta
    25321 gtcgtcctgg cctcgcccgg taaagtcacc cactcggaca cagggttggg cccagtcaag
    25381 gtcgacgaat tcattgcttt ggttggtgat cgtcatgtga atgtcgacct cgtcgtatcc
    25441 ggcgcggatt tcgtgatcga tcaccacctt gccgtcgaca atatgcctca gcttgaccca
    25501 acggccatct gggtcggcgg ccacaagttg gctttcgaag gggatagtcg tttgagacca
    25561 gtcccggtcg gtgctgcccc ttcggcaaaa ggcctccagg taccaagtct cgatcttgcc
    25621 ccccggcaag cggtcggatc ggatggtgag gatatcccgc tcacctttcc gccagtcgat
    25681 ccgcagaccg tcgttgatgg gggtggcgca tcctgacagg ggggcaagca acagcagacc
    25741 tgtcaatgat ttccgcattt cagcatcctc cagcttagaa ttcgcgtatg ctactgtaag
    25801 tccgaggcat gatcgagagt ctaacagggg tagtcatggc cgagcgcatg acatgccggc
    25861 tgggacgaga gtcctacccc gcggacggcg aggacgcagt tctagccggg ggtctggcgc
    25921 gtcgtgatcc ggtcgtcttc gaccaactcg tcgagaccta ccaggcccgg gtggcgaggc
    25981 tggtgtgccg gctgctcggc tggccgcaag acgtggacga cgtggtgcag gaggtgtttt
    26041 tggcggcatg gaagggcctg ccccggttcc gcggacaggc ggagttgggt acgtggctga
    26101 tgcggatcac cattaacaag tgtcgtggcc accggcggaa ggaaatgctc agactgcgtc
    26161 ggttttggag gagcaaggaa cggcaacaga tgcgtaacgc tgtctcggct gatggggcga
    26221 ccctcgacgc ggagaaccag gctgaggttc gtcgtgcggt gcggaggttg ccggcgagat
    26281 actgcgaggt gatagtgctt cgatacctcg aggagatgcc cgtcgagagg atcggacagg
    26341 tgctggggct gacgcggaac gctgttgagg ttcggttgca tcgggcaaga gcgcgattgc
    26401 gaaaggagtt gggcggattg ctggaagaat gagtgggttt ggcgtcgcct tgtgtccccc
    26461 ttgccaaggg agaaagcaag aggcagttgc caggtagggc cgacgccctc gtcggccccc
    26521 cccgatcccc cttgcgaagg ggggaagagg atgcgagatg actcagacgg gccgctctgc
    26581 ccagaatcct ttccgggcca cttggctcgg ccgaatccgt tcggcattcg agcgaggata
    26641 ggaatcctcg acgccgaagg atctggaacg gattccgggg ccggcgtggc caccctcaga
    26701 gtgccaagag tgccgacggg gcgtcggcac cacgtgaccc ccgtcggtcc ccccttgtta
    26761 aggggggaag ccaggtaggg ccgacgccct cgtcggcccc cccgatcccc cttgcgaaag
    26821 gggggaaggg atgagcgatg agcgatgaac gactccaaca actgttgcga caggccgacg
    26881 cggccttcgg cggcccgcgg gtcagttctg ctgacttgcc gctgagggtg cgacagcggg
    26941 cgaggcggca gcatcgggcg aaggtgttcg gcaccgccac agcagcggtt ttggtgttga
    27001 gcctgaccac ggcggcgtgg gtcaacaggt acggccggtc cgtcccctcg ccgacgccga
    27061 tcgttgctga cgacggctcg aagccggcgt cggtggcaga gttgcaatca catattgagc
    27121 agctcagcga agagatacgg accggttgcc aagccgtcca cgaggtctta cggcgacagg
    27181 aggtcgagca gcgcctggcg gccttgcgga ggcaggcgga ggcggacccg ctcaaggaga
    27241 tcaatgacca gatagaccgg gccgcgctaa ccatggtcta tcaagccgac cggatgtatc
    27301 gcgagttgaa tctgcgggaa tcggcgatag cgtcgtacga acggaccatc aagcttttcc
    27361 ccaagactca ttgggcagag gtggcaaagg acagactggc cgaactcaag acggccggaa
    27421 acggagatgc gatatgaaaa ccggaactct ggaaacaaac gtggtgatat cactgtttgt
    27481 tggtggtctg gcagcctccg catggggcca ggcggttccc agtccgtcta tgcatgatcc
    27541 cgatagtccg gtgaattgcg tcgtcaagat cgcctgcgac ccggcgcttc tgcccctcaa
    27601 cccgcaatct gtcggggcac tgctggagag caggttcgtc gccggcgagg cgctcaagca
    27661 gactcttccg ccggaggcct tcttatccta cacagtcgaa ttcagaccgt tgccgggggg
    27721 aacgatcgca gcccccgtcg gggtgacgcc gggcatgccg atgatggatg accccctgcc
    27781 gcagcccggt ggggtcttct tgcccgctcc cccaggcaga gatggcatgg gtggctacgg
    27841 cgacgagggt ggcggcggcg agcgggccgg tagtaggagc ggtggtccca gcggttccaa
    27901 cgccccggcc cacggtggta gatatggcgg ctatggcggc ggtggcagga caggcccagc
    27961 cggcggtccg tgggcgggac cggagaagcc tgacgcacct tcgggcggga cgccgatgcc
    28021 ggcaggagcg gttccggatc gttccgttgc accgggagga ccccgaccag gtggccggcc
    28081 cgtgatggtc gacatcccgg aacccggccc cggggagggc agcatcattg ggcagatcga
    28141 ggtacaaatt gacggcgcgg aaccccacca taacgaggcg tttctgcgag cgatctgcga
    28201 gcggctgcaa caggcgctgt acggcttgtg ggagagcgag atgagttcga tcggccggca
    28261 gctcgagatg agtcgggatg aactgctgcg agctgaaaag aagctgcgag aactgcagga
    28321 acggcggcga gacctgcttg gggccggaca ggtcgacctg acacccgaag cggtgatgga
    28381 tcagattcgt cagctcgacc gcgaacgcca gcgaatggaa atggatctgc tcggacagca
    28441 ggctcgcctc aaggccgtcc agcagcagat ccaggcaacc agtgagcgga gcctggcgag
    28501 cccgggcggc gatgaaatga tcaagccgct ccaagccaag ctcgaggtgt tgaggaacga
    28561 actgaaacga gtggaggaga tgaatcagcg gggcatggtt cccggagggg acgtcgagaa
    28621 ggcccgtgtg gccgtgttgg aggcggaagt ccaactggtt gaggcccggc gcaaggcggc
    28681 cgaagtcggc ggggtcgagg tcctcggcaa gctcaacacg gaactggcga tgttgtctgt
    28741 gaacgtcgca gaaaccgagg ctcgtctcac gtccctccgc gaacagatcg agaaggccag
    28801 gcagcttgtg ggcgtagtgg acgaatatga aatgagagtc ctgatggaga tgccccacgc
    28861 caggcgggcc tacgagtcga gcaaactccg tgtcgaagaa ctggagcagc gaatccgcac
    28921 ggccgtggcc cccacggtga cggtcatcgg tggtgcaacc tcgaagccgg cgaagtgagg
    28981 agtggtcagt cctcagtctt cagttgtcag ccgatggtag agcatcgagc ccgccccgcc
    29041 ctggtccgct gtggcgaccg caggcagggt cccctctgcg accgaaaccc gccaatgact
    29101 ccgcgccgat cggcaggtct ccgccgcctt cagcggctac gaccgtgctc tacctgctga
    29161 gaggttcatc gataatcggc aagccagatc atccgatcag caagctgttc accttggagt
    29221 gttcccacga gccgaagggt gcgcctccat aactgcgaaa acgggagccg ctcgctgaca
    29281 ataagtcgcg agaagtgcct gtcctcttcg atcgccctcc gcgccaaagg cacgaagtcg
    29341 cggatgttga atgtcagcag ggcctgtccg ttagctgcac aatactcaaa atgatcggca
    29401 tcgggtatcg acagacgtcc gagctcgacg gcgcttactg catcgtagcc gcgttcacgc
    29461 aataccgcac cgagcaaggg acgaacatcc tcgtccagcc aaatccggat gaggctcatc
    29521 acatttcctc tcgccaacac gcctcggtgt tatcctccat ctcggcttcc agttcagcct
    29581 ggtgatcgta gtagtagctc aacgcgtcgt acacagcggg cagggagagt tgtgggaact
    29641 ccctaaggcg gaccgccttt ttgcgggccg caacccaagc gtactgcgat ccagaaacat
    29701 gcgcgcaaaa tgcgcaccag tcgcggcgga agggacta
//
LOCUS       DAOTPW010000032        29675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1637_length_29675_cov_5.415800, whole genome shotgun sequence.
ACCESSION   DAOTPW010000032 DAOTPW010000000
VERSION     DAOTPW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 29675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29675
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1637_length_29675_cov_5.415800"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..629)
                     /locus_tag="P5316_04585"
     CDS             complement(33..629)
                     /locus_tag="P5316_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84265.1"
                     /translation="MVEDWEVSRTTGRCAASDRVLAEGEAYYAVLLETASGFERRDYA
                     VDVWQGPPEGTFCYWRGRVPVREKKPGTYAVDMAILSQLFTQLEDAQSESKQQLRFVL
                     ALLLMRKRLLKMDRSTTEEGREYWHLRMSSDQTVHRVLNPRLEKEQIERLSAQLTAIL
                     SGEVDALQVVEQPDTEPSPAGDEENRSYGAVETDAQST"
     gene            complement(1213..3393)
                     /locus_tag="P5316_04590"
     CDS             complement(1213..3393)
                     /locus_tag="P5316_04590"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRR84266.1"
                     /translation="MRSLPVTLALLGFAAPAAPATTFIVVNRGEYKSAEQAGNDEANV
                     KWDDGDDADDVICTENFAALELQRYLRMMAGPEGQEDYRIVDDDHLPAKGEIILLGNP
                     QTNKAVKLPPADFGDLGPEGYIIRSAERSTHIGGERRIGTLYGVYRFLDHLGVRWLSP
                     GKIGEEIPDRRLRSLDRSLNIKEKPAFVSRGFHAWEDRGDPEFFDWMARNRMNYWCVQ
                     VPDKAALKKRGIQMNCGGHLLTHRFISPDAAYPYNHSGFAGDDDRPADPYPVSPDFKG
                     DADEDGKLSYSEAHPEWYGFIGGKRSFRIRDDFGDNFCTSNHHACDEFFKNLVKDLID
                     GEWKDADSINFWTLDAGKWCQCDSCKTLGTPTDRNLLLVHRLRQETERATQENRLHRN
                     IRIYFLAYSDVIEPPTRPLPDDFDYENCIATFFPIRRCYVHRFDDPACTEFNRSYMNH
                     FINWFVNPDRHYRGQIFIGEYYNVSRYKCLPIVFERTMSVDIPYYFEQGARHMHYMHC
                     TTRDWGTRTLTNWQFARMLWDPKLDSKALLDDYFAHRYGPAAGKLRELYGILDTALCN
                     ATPLKYHLVDRLNRDNKELFPTRHLKHEAARFDADDGPDLVEMVASIDKCVKLMEEVQ
                     SQQWPERVRSRIMEDAGPIRYAADTLHFFDALVRARRLVQAGRRDKAKTLLPEMKRLA
                     KALEADTTSMKWSSSHASAANGLEASLVRKAYEQMVSELTEDPR"
     gene            complement(3580..5550)
                     /locus_tag="P5316_04595"
     CDS             complement(3580..5550)
                     /locus_tag="P5316_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRR84267.1"
                     /translation="MMQSLVIGLCVIVLLVTSGLAAEPANKHYPVNREPLLQSKFVPL
                     PLGTVRPEGWLKDQLTVQANGLTGRLDEFWPSLANNAWRGSKDKDAEAWERGPYYLDG
                     LVPLAYLLNDQRLIAKVKSWIEPIIASARPDGWFGPEKNTDRWPLAVAMKVLIQYQEA
                     TGDPRVIPLLQNYFNYLKAAPPDWPDKEWRGVRAMENIITAYWLYNRTGDKTILEVAD
                     SIYNNSFKWTDYFLNFPYTDDVMAMGVKYGHPTHVVNIAMAVKHPGMRYIQAHDPRLV
                     EAVKTGLASLDKYHGQVTGRFAGDEHLSGRRPTQGTELCAVVEFMFSLENLAAQLGDV
                     YYADRLEMLAYSANPGTCTPDYWAHQYDQQANQVLVSVAKRRWSTNGDASNVYGLEPN
                     FGCCTANMHQGWPKFVAHLWMATHDQGLAVIAYGPSIVKAKVGEGVEVTLVELTDYPF
                     DGLVRIRMDSPQPAKFPLHLRIPAWAAGARIKTPEQTVDTRPGSFAVVDRLWKQGDML
                     EVTLPMNLRTETRYNNAVSILRGPLYFSLKIGEQYKEIKRHHETLPAIDWSIEPTTPW
                     NYGLLVDRENPEKSIAVEKRSISKLPFDQRNPPVVLKAKGRLIPQWGMVDNSADDPPV
                     SPVTSDQPLADVELIPYGNTRLRVTEIPVLAQ"
     gene            complement(5765..7096)
                     /locus_tag="P5316_04600"
     CDS             complement(5765..7096)
                     /locus_tag="P5316_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84268.1"
                     /translation="MSRITRRHFINSSMAAAASSAVWSSLAAPRSVLGANEKIRVGLI
                     GSGGMGRGDILTFLKYGDVECPIICDVDDKMLAEGVKEIEKARGKAPETVKDFRTVID
                     RKDVDAVVVATPDHWHAIPTIMACQAGKDVYCEKPLATSVGEGRAMLNAARKYNRVVQ
                     MGTQWRSNPDFTEAIAYVHSGKLGKIRMVRCWAYLNWVHSVGKPADQDKAPDGVDYDM
                     WLGPAPKRPFNPARFHFSFRWFWDYAGGLMTDWGVHLINIALWGMKVGMPERVSSSGG
                     KYIYDDISDTPDTQFAVYEYPGFALIWEHQMTAGCGVDGKEHGIAFYGTEGTLTVGAY
                     GWEVAPAVKDKRVPSEEEPLPAEKHESKFAEAGRPQHVRNFLDCMRSRQRPVEDFELG
                     HAISTAAHLGNLALRSRSTIIWDAKNERPVGRTDLDDLLRYPYRAPWKLEV"
     gene            7393..8253
                     /locus_tag="P5316_04605"
     CDS             7393..8253
                     /locus_tag="P5316_04605"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRR84269.1"
                     /translation="MRGKQIISVLIPVVSLWPWIALAEFPGERPYPGITYVQETRQDP
                     PMRLFIAQVDLADPKVRVDVAPGGPDPDGPGEWETTLMVPSKVASRERFDLAVNGDFF
                     SIPKPASSAAQPSYRPEVWANAIGPAVTDGKAWSVAEKKTPCLIVRKNGRVSIEMIDR
                     PPSDAAEVVAGNVMLVENGKPVRHESKARHPRTVVGLDKKKNRLTILVVDGRRPGASI
                     GMSYEELAKEMIRLGCHTAVNLDGGGSSVMVMRDPETNEYKILNKPSDGRERPVANVL
                     GITVGNGEKK"
     gene            complement(8337..9464)
                     /locus_tag="P5316_04610"
     CDS             complement(8337..9464)
                     /locus_tag="P5316_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRR84270.1"
                     /translation="MNVRQLRELFPITRRYNFLNAAAVAPLSSPASQAMCRYIEHTCG
                     QATIGADFYMNAEQTRSLAAALIGADPDEVTFTKNTTEGIGWVAGGLPWRAGDNVVIT
                     AVEFPANVYPWMGLGRQGVDLRMVKEVDGRVPAEDVIAAIDERTRVVSVSAVQYASGF
                     RMDLPRLGQVCRERDVLLCVDAIQALGVLPIDVRAMQIDFLSADGHKWLCGPEGCGIF
                     YCRRELLDRLTPVFAGWLCMEDALDFGNYRFEFLRSARKFDTGSYNLAGICGLGASIE
                     MWLEAGIDVVAQWALALTDRLVEGVRGKGYRVVSSRLPGEASSIVAFVSDHHDHGEIR
                     KRLQEQHRVIISCREGRLRASPHAYNTLEEIDQLIELLPGH"
     gene            9775..11055
                     /locus_tag="P5316_04615"
     CDS             9775..11055
                     /locus_tag="P5316_04615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HRR84271.1"
                     /translation="MSRSASFIRIAILSSVVPACLVGCLPLDFSGWGDQVLSFPAPQP
                     LSGPQINEVEPNDDMAAANAVVFDGQVTLVGSIAAKGSFDFDVFALGPVNAGDRVVAE
                     LAASAGSDVVLGLFDGGFNLLGYVDYSRGTAAPGRIDLVLRDSVDTLYAIAGTRSLSE
                     ATRLYNVGISVTPNVGVPQPNPQMVVLNFIGASGVRIGRQAPVNVPPFDVATIDSRFA
                     GKTQEVIQSILQMVREDYAGLNVVFYLAGDPNIPAGDLTVIHFGTYSDRYLGLADNVD
                     PFNRDSTQNAIIYTDTFSLFSTLWPTQEQIAQVLANVASHELGHLLGLRHTADIQDLM
                     DITASARQMLSDQWHKVADLHPSIMAIGYQDAATMLSWCVGGTPPAPPANLSAKTQRM
                     AKTAYDPDDFYIPRGWLAENSAEADEAVEDPAEE"
     gene            complement(11085..13361)
                     /locus_tag="P5316_04620"
     CDS             complement(11085..13361)
                     /locus_tag="P5316_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR84272.1"
                     /translation="MAPQKRIGRRLLITFAVLCAWAALPAWGVQITRGPYLQLQTPDS
                     VSVVWFTDSTCTGEVEWGLTASYGNLAQSTQPGTYHEIAVTGLSPDTLYHYRARSDGT
                     PLSGDATFRTAPPPGASAVSFSFVGDSCSAPSNCAATYNAMLSQSPNGFCLTLGDLAG
                     RGEDNITDYWQSHFFTPAAGFIKHICMYTAIGNHELYDETATYVYPARYLANWSLPKA
                     SSGTEFYYSFDKAHVHFASIDTFWSSYGVGSAQSNWLAGDLTGTTQPWKIVFGHNGPY
                     VSEDGGSNGSTTMRTSLVPLFEQHGVDLYLHGHYHNYQRNVVNGVCYIDQGTGGQPWS
                     TKHADDSQPYVRAYADQYYCFTRLDIQGSRLLGRCLKTSDGTVLDGFQIDKPAIAMPW
                     QDAFPAAGPQLNWIAPWNFGSQCGLVARPGNPSGDGYVFEVADTSGHQYAYPMLADEA
                     LTNQSIEAQVYYDNSTSVKNRFGVGLRGRLFFSAAERSYYALAFVRNDNLAADGHCVL
                     IRRQNEIETVLANWTYPDVAGWHKMRLSMEGQELSVWVDDQLLTATPIHDSSLLKGRP
                     FIYNYRSSISGAKTLVDNVTIGPVVEPPVISQHPVSQTVYLGETAVFSVTASGFPPVS
                     YQWQKDLIDLAEAGHCSGVTTALLTVSNADGSDVGFYRCVVSNSEGSAASNAASLTVE
                     GPRPVQADFDQDGDVDQEDFGHFQSCLSGSGTSQNEPRCQNAKFDGDNDVDESDFALF
                     RQCISGRGVSVDPSCMKS"
     gene            14081..15178
                     /locus_tag="P5316_04625"
     CDS             14081..15178
                     /locus_tag="P5316_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRR84273.1"
                     /translation="MDRLLRGMGQSSGEGGQAHARATDLHINPGRPPDYRIARVIRSV
                     AARPLSQKDVILLSNAIMNPAQQERFNRNHCIDFSYHVDRNRFRVNIVESFIGRSISI
                     RRFEEIKNFEDYNLPSNYIDICENARGFVIMSGSTGSGKTTSIAAMLDHVNRNFHRKI
                     VTIEDPIEYVYTPKKSIVVQIEIGEDGIPNEDIALRNLVRMDPDAGLAGEMRDRQSLE
                     AALKTAQAGHLIFGTLHCEGVGQAFDRVLAYYDTIEQQRILGTLADNLAAIINQRLLP
                     CIKPGIDVVPAYEIFRPNPLTRRYIRERRYTEAVAEMEKQSMKELGCVTYVDSLYKLV
                     TEEWIDYHTALEHSGDQAEKLKAKLKGIDMK"
     gene            complement(15284..17017)
                     /gene="polX"
                     /locus_tag="P5316_04630"
     CDS             complement(15284..17017)
                     /gene="polX"
                     /locus_tag="P5316_04630"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HRR84274.1"
                     /translation="MVNPEIAAVFNEIADLLELTGGDPFRVNSYRKAARSIEDLTEDI
                     ADVANRGELQNIPGVGKGTAERIMQYLKTGKVAVHQELLNSIPRGLPELLTVPGLGPK
                     KVQALHRELGVNSMADLEAVIADGRAERLPGFGKKSVEKIAEGLAFLKRSAGRTPLGA
                     ALPLAEGLRDAVREFTGVRRIEIAGSARRGCETVGDIDLLCIADDGERVVNAFTKLPE
                     VTGVRAAGGTKGSVLVAGPVRGEIQVDLRVVPEESFGAALQYFTGSKEHNVRLRELAV
                     RRGWKLNEYGLFDGDRRIAGREEEEIYEKLDLRPIPPELREDRGEIECRGEIPRLVSL
                     ADIKGDMHVHSTASDGRSTIEEMAEAAGKRGYRYLAICDHSKSSVIANGLDETRLLRH
                     IQDIRAAGERVKGLTLLAGIECDILANGSLDYPDDILAQLDWVIASIHSAQQQDRASL
                     TRRSIAAMENPYVCCLGHPSGRLLGRRDAMDLDWDELFATAARTGCALEISASWQRLD
                     LKDVHIRQAIDAGCRLAISTDAHSTDQLDQMPLGVQTARRGWATPEHILNTLPVARLR
                     AWIAAKRKRRM"
     gene            17150..18205
                     /gene="lpxD"
                     /locus_tag="P5316_04635"
     CDS             17150..18205
                     /gene="lpxD"
                     /locus_tag="P5316_04635"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRR84275.1"
                     /translation="MGLTLTQLTEFLASQSIPCEVDGDGSVVVSSVATLEDAGEGQLS
                     FLSNPKYEKELAATRASAVLVRPDVRVSRTMNLLRTPDPYAALTAAIVRIHGYRQHPQ
                     WGRSAQACIHPSAVIGEKPNIAPGVHIDENVRIGRHATIYPGVYIARGCRIGDDVVLM
                     PNVVIYEACVLGNRVTIHAGSIIGEDGLGYAPVGEKWVKIPQIGNVVIGDDVEIGANC
                     TIDRATLGSSVIGSGTKFSNLIAIGHGTKIGEDCLFVAQVGIAGSVSVGRHVTMAGQA
                     GVVGHISIGDNAVIGAKAGVTNDVEPGLTMLGQPAVPINECKRQVAIVQRLPQLKNEI
                     KRLRRDLDQLMKQVGGG"
     gene            18202..19074
                     /gene="lpxI"
                     /locus_tag="P5316_04640"
     CDS             18202..19074
                     /gene="lpxI"
                     /locus_tag="P5316_04640"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRR84276.1"
                     /translation="MSDHLYNPLGIIAGAGRFPFLAAQGAHQAGRAVVIVALRGLANP
                     ELRTVADRFHWSGIVRLGRWIRLFRKSGVNEAIMAGSVIKSEMYGRLRILRYVPDWTS
                     IRLWFFEVADRRNDTILRAVAEHMQREGITLTDSLQYCRKHLPAPGVLTRNRPSKALD
                     KDIEFGWHIAKEMGRLDIGQSIAVKEQEVIAVEAIEGTDRMIERAGELCRHGGWTLIK
                     VAKPNQDLRFDVPTVGPNTIENLHRHGAKALVFEAGVTLAIDVNQMIALADRYGMIVV
                     AHEPGNATHTQQTA"
     gene            19720..21051
                     /locus_tag="P5316_04645"
     CDS             19720..21051
                     /locus_tag="P5316_04645"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRR84277.1"
                     /translation="MHSPDELIALLREEIRQRREEGCNVVGHAQRMRKAGKRIDELNA
                     LYDDLMTLKPEPRLARREPNDLAGIRRLRPRRPRRIKSPASTARLRDRLRGAFLGRCA
                     GCMLGKPVEDWHRDRIFKALKKADRFPLAYYFESSTIEAGGKPIHPAGVTYCTLGHFK
                     HMERDDDTDYTILGIHCLKTYGPKFTTANVAHEWQMRLPYFMTYTAERAAYRNLVNRL
                     PLDQVPLYRNPFREWIGAQIRADGFGYCAAGMPELAAEFAYRDATLSHAKNGVYGEML
                     FAAMIAAAAVCDDVREVIEIGLSEIPAGCRLATAVRDVTRWADENRNWQDTLHAIHDR
                     YGHYHSVHTINNACLVIMGLLYGQMDFGKTICITVMGGWDTDCTGATAGSVLGAMLGA
                     DRLPRRWIKPLNNRLESIVIGYNDVRITDIADAAFEVNRKIRLGGPRFSKG"
     gene            21749..22189
                     /locus_tag="P5316_04650"
     CDS             21749..22189
                     /locus_tag="P5316_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR84278.1"
                     /translation="MQVRRYNRISPFEQLRREIAGLLDEFDPLGVRGALFGSPGFPAI
                     NVWESGDCLYAEAEIPGVTQEDLEILVVGNELTIKGRRGTAEEEDASYHRRERGTGEF
                     SRTVTLPVDIKAEAVDATLKDGVLTLSLPKADEARPRKITVKEV"
     gene            22348..22749
                     /locus_tag="P5316_04655"
     CDS             22348..22749
                     /locus_tag="P5316_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR84279.1"
                     /translation="MRKHKAIVRTEGDVAQAEQTRDRQVYSPQVDIIEKDNGLLLLAD
                     MPGVKAADIDIQYEDGQLTIYGKVEPRQDDSKTSYLLHEYGVGDFYRSFRIGDGIDVD
                     SIRAELKNGVMTLHLPKTEAFRPRKIAVQAK"
     gene            complement(22800..23036)
                     /locus_tag="P5316_04660"
     CDS             complement(22800..23036)
                     /locus_tag="P5316_04660"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HRR84280.1"
                     /translation="MRRIWRLIWRILVAIVGSAVVLAGIGLSIPLIPGPGFAVIIAGL
                     AILATEFSGPRRLLRWLPRRIRQWKAERARRQAR"
     gene            complement(23107..24144)
                     /locus_tag="P5316_04665"
     CDS             complement(23107..24144)
                     /locus_tag="P5316_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRR84281.1"
                     /translation="MLLLWIGFVTLVVLMLVFDLCVLHRRAVVIGLKQALLWSAMWIA
                     VALSFNVALYYIYKHDWMGIAAQRIASDPARYPMPVAQADAKVAATAAKEYFGGYILE
                     KSLSVDNLFVMAVIFSFFGIKAEHQHRVLFWGILGALVMRGIMIGGVTWVVHHLHWMT
                     YVFGLLLILTAVKMLFSKEEHIDPDRNPLVRLVRHFYPVTSQFQGARFVVRQNGRWAI
                     TPLLLALLVVETTDLLFAIDSIPAIIGLTADTFIVFTSNIMAILGLRALYFALAGMMR
                     EFEYLKLALVIILAFVGVKMLIVAFGIRLDINLSLAIIVTILTIGVVASIIAGKRQKG
                     TTNDGVTGTTA"
     gene            complement(24303..25508)
                     /locus_tag="P5316_04670"
     CDS             complement(24303..25508)
                     /locus_tag="P5316_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HRR84282.1"
                     /translation="MSRTSASLEQTAEAKAAAMEVLLNNVRGPCQRLPRTAAWGYPEP
                     YTRDMMISALGFLSSGDSVLRDALRRVLLSLARTQTPHGHIASLAHDPKDLGASDTTP
                     LFLFGLGLYRKVTGDKRFLRNAAMKALRWMEYQGADDMVMVDQQPTTDWRDEQWVLGY
                     GLYVNTIVYACLRLHGQHRKADLLRCLIDHPDLRGSKRQRYVHEGFVIPGKPYYALWS
                     FKVYHSRRFDLLGNSLAILTGIAPQARAQRIINWTETQCRRLRRRGELAVDLPPCLIP
                     YIQPEDQDWRPRYQRYNRPGNYHNGGVWPFVCGFYIAALVAAGRSRLAAQKLAILTEL
                     VRPAKKAKVSYGFNEWIKAQTGRPRGHDWQTWSAAMYLYAAECVEQGRTPFFDEIRRL
                     GRSSQKPDE"
     gene            complement(25505..26647)
                     /locus_tag="P5316_04675"
     CDS             complement(25505..26647)
                     /locus_tag="P5316_04675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR84283.1"
                     /translation="MKALVLYDYPPSPGGLATQGDLLYRGLQQLGVDVYPVNFESAQE
                     KEWYYRWFKPDVVVGVGYWGHTPHLILHPQRHGVQAVPWLVADGFVANYREVLNSLPL
                     ILTTSEWVKNVYIRDGINGENMCVLPVGCDTDSFIPRDISDAKVRVVREALGVEPDDL
                     MILTVGGDAASKGAQEVMQALALIAKDVPKWKYVCKVWPQQRTEIQNRMDRELATQLG
                     IEKNVIYTTNKVSRNFMPYLIAACDIYAAPSRLEGFGMPQVEAGACARPVVSINAMAP
                     AETLVHGETALLAGVAQEIRIAEAILGEESGFNKAQRVVFENLRIVDYRASIYDIAEH
                     LKTLMTDADLRARMGTKGRERVVSKYDYRVVARQFVDYVREKLGIQ"
     gene            complement(26644..27312)
                     /locus_tag="P5316_04680"
     CDS             complement(26644..27312)
                     /locus_tag="P5316_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRR84284.1"
                     /translation="MLLDTILDSSYRPPRVAVIVAHPDDETLWAGGQILARPDWDWTI
                     VTLCRGNDPDRAPKFRRVLERLNARGAMADLDDGPEQTPLPWAEVEDAILSLLCGTAF
                     DAVLTHGPWGEYTRHRRHEEASQAVTRLWRNGRLTAGRLFLFAFEDGQRAYLPRPRAN
                     AHLVETLSQEIWQQKYDIITGLYAFAPDSWEAQATPRQEAVWHFESPDALNGWIGSEE
                     GSCR"
     gene            complement(27317..>29675)
                     /locus_tag="P5316_04685"
     CDS             complement(27317..>29675)
                     /locus_tag="P5316_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HRR84285.1"
                     /translation="ESFERYVDSFRRKGKGPHFNYNSWWTAPVPFSEKDVLSLIKTFE
                     DQLYRPFGAAMDSFTLDMGWSARTGIWQIDRSRFPGEFATLNRALAAQSCRLGLWWSP
                     SNNYTPQSFDSLWAAKQGYETQSLASSTIRDYRVCCLAKGTRYQAAAKEAIAGLANRF
                     KLGQMKFDGYRHECKEADHGHLPNELSREVTAEGIIDIFAAVREASPDIWMEPTCFGY
                     DASPWWLRYVESVIGPFGDDAPAGAVPAPVYRESYTTSRDFYNLRGAATPVPIAAQEV
                     LGIVHQTPDPLCNDAVVTVLRGHQFISLYLNPKHMRPDEYRFLAELMTWSRANASLLE
                     RTKLVWPRTWREKGLPTVHDTKAMQRETYGYAHWNDGQGLICLRNPWIQMDLVEVLLD
                     EATIGADVRFERYSAIQEYPHRRCVAVGLNHGDRLTLSMGPYETKVIRLCPSIREDLP
                     DSSSGQVCGAKVASAKSKAVKTTAQSPGTQPFGDDYTIVTPPEGLRWTSTIEGRSPGR
                     GWKVYYLLESDKSPEVLDPVFTINGLAAESTLIDSAGQWCASGLKSKHGWKWYVVDLP
                     SGQWQVSAAMNTPQHDISASAWLVREFDPPAAITQPGPHKGRMPPFPLPPDCRSQISV
                     VAIAPTRLLTPDMEVITFEPRIKRIPGIYLDRLEPVSVKQGWGRLMKNKSVWDKTMCI
                     GSQVYHRGLGTHAPAEVVYDLGGKYKAFHGLAGQDAAANGTITVEIHVDGEKRWDSGR
                     MARHEPAREFSVSVAGAKTLTIIVTDADDGIVGDHADLADAWLEP"
BASE COUNT         5753 a         9115 c         8999 g         5808 t
ORIGIN      
        1 gcagccggcc cgcaccgggc ctgaatgagc gatcaggttg actgggcatc agtctcgact
       61 gctccgtaag atcggttttc ttcatcgccg gccggggatg gttcggtatc cggctgttcg
      121 accacctgca gggcatcaac ctcgccgctg agaatggccg tgagctgggc gctgagccgc
      181 tcgatctgtt ccttttccag ccgggggttg agcacccggt gcacggtctg gtcgctgctc
      241 atccgcaggt gccagtactc acggccttct tcggtcgtac tccggtccat cttcagcagc
      301 cgtttgcgca tcagcagaag ggcgaggacg aagcgcaatt gttgcttcga ctccgattgg
      361 gcgtcttcca gttgtgtgaa cagttgcgac aagatggcca tatccaccgc gtaggtgccc
      421 ggcttcttct cgcgaaccgg cacccggcct cgccaataac agaacgtgcc ttccggtggg
      481 ccctgccaga catcgacggc ataatcgcgg cgttcaaacc cgctcgccgt ctcgagcaac
      541 accgcgtaat aggcctcacc ctcggccagc acccgatccg acgccgcgca cctgcccgtc
      601 gtccgtgata cttcccaatc ctcgaccatc gatcctgctc gtgcctacct gtgaccgtga
      661 taccacccgg ccgccagaga caaaggtaca cgatccgcgg caaggcgtaa atgcgggagg
      721 gtccgcgccc acaagccgga atccgggacc agcgaacgga aatcatcgaa ccggcaactt
      781 ggcccggggc ggtaccatgg ggctcagggg ttgggatacc tcgccgccgc agtgcctctc
      841 tggacaaaca taaggcagac cgcctttttg cgggccgcaa cccaagtgct ctgcgatcga
      901 gaaacatgcg cgcaagccgc acccctctct ggccgtaagg ttgtaaagca gatcaccagc
      961 catgccggtc acggctgggc ggcaacctga atctgatgct tgaggatgct cggatggcga
     1021 atggccgtgg cgacagaggg ccgccaggtt ctcaggctgc caccgtgggc ataccttcgt
     1081 cagaagcttc aatccttgcg ccgcatgagg agccgatgcc tccggcaccg gggtcgttgt
     1141 accgagttcc tcatccacca acgaaccctc gatcaggcaa tccatcgcca tcaccttggc
     1201 gctgcccggg cttcatctcg ggtcttcggt caactccgat accatttgct cgtaagcctt
     1261 tctgacgagg ctggcttcca ggccgttcgc ggcactggca tgcgaggatg accatttcat
     1321 gctggtggta tcggcttcaa gggccttcgc cagacgcttc atttcaggca gcagggtctt
     1381 ggccttgtct cgacggccgg cctgaacaag ccggcgggcc cggacaagcg cgtcgaagaa
     1441 gtgcaacgtg tcagcagcgt aacggatggg gccggcatcc tccatgattc gcgaccgcac
     1501 gcgctccggc cactgctgcg actgcacttc ctccatcagc ttcacacatt tgtcgatgga
     1561 tgcgaccatc tcgaccagat cgggcccgtc gtcggcgtca aaacgcgcgg cctcatgctt
     1621 gaggtgacgt gtcgggaaga gctccttgtt atcacggttg agccggtcga ccagatggta
     1681 tttcagcggc gtggcgttac acaaagcggt gtcgagtatg ccgtacagtt ctcgcagctt
     1741 tccggcagcc ggaccatacc ggtgggcgaa gtagtcatcg agcagggcct tgctgtcgag
     1801 cttcggatcc cacagcatgc gggcgaactg ccagttggtc agagtcctgg tgccccaatc
     1861 cctggtcgtg cagtgcatgt agtgcatgtg ccgggcgcct tgctcgaagt aataagggat
     1921 gtccacagac atggtccgct cgaagacgat tggcaggcac ttgtatcggc tcacattata
     1981 gtattcgccg atgaagatct gcccgcggta atggcggtcc ggattgacga accagttgat
     2041 gaagtggttc atgtagctgc ggttgaactc ggtgcaggcc ggatcgtcga agcggtggac
     2101 atagcagcgc cggatgggga agaacgtcgc gatgcagttc tcgtagtcga agtcgtccgg
     2161 cagcggccgg gtcggtggtt cgatgacgtc ggagtatgca aggaagtaga tgcggatgtt
     2221 gcggtgcagg cggttttcct gggtcgcccg ttctgtctcc tgtcgcagcc ggtgcaccag
     2281 cagtagattg cgatccgtcg gggtgccgag ggtcttgcat gagtcgcatt ggcaccattt
     2341 gccggcgtcg agcgtccaga agttgatgct gtcggcgtcc ttccactcgc cgtctatcaa
     2401 gtctttcacg aggttcttga aaaactcgtc gcatgcgtgg tgattggagg tgcagaagtt
     2461 gtcgccgaaa tcgtcccgga tgcgaaagct ccgcttgccg ccgatgaagc cgtaccattc
     2521 ggggtgggcc tcgctgtagc tgagcttgcc gtcttcatcc gcatcgccct tgaaatcggg
     2581 gctgaccgga tatgggtcgg ccggacggtc atcgtcaccc gcaaagccgc tgtggttgta
     2641 gggatacgca gcgtccgggc tgataaaccg gtgcgtgagc agatgcccgc cgcagttcat
     2701 ctgaatgccg cgtttcttga gggcggcctt gtcgggcact tggacgcacc agtagttcat
     2761 ccggttacgg gccatccagt caaagaactc cggatcaccg cgatcttccc aggcgtgaaa
     2821 cccgcgcgag acaaaagccg gtttctcttt gatgttcaag gaccggtcca gactgcgtag
     2881 ccgacgatcg gggatctcct cgccgatctt gccgggtgac agccaccgga cacccaggtg
     2941 atcgaggaag cggtagacgc cgtagagtgt gccgattcgt cgctcaccgc cgatgtgcgt
     3001 cgaccgctct gcgctccgga tgatgtagcc ttccggtccg aggtcgccga agtctgcagg
     3061 cggcagcttc accgccttgt ttgtttgcgg gttgccgagc aggatgatct cgccctttgc
     3121 aggcaagtga tcgtcatcga cgatccggta gtcttcttgc ccttccggcc cggccatcat
     3181 tcgcaggtat cgttgcagtt cgagggccgc gaaattctcc gtgcagatga cgtcgtcggc
     3241 atcgtctcca tcgtcccact tgacgttggc ttcgtcgttg ccggcttgtt cggcggactt
     3301 gtattctccg cggttgacca cgatgaaggt agtggcgggc gcggccgggg cggcaaagcc
     3361 gagaagggca agtgtcacgg gcagagaccg catgcgtgat gctcctgtgc tgcgagacca
     3421 cgggtgagaa atccagaatg acgaaatccg gatgacgaat gaagcccgaa tgatgaacgt
     3481 cgaacaccgg ccttgggcaa ttcgacattc gggtttcgtc attccttcgt cattcggatt
     3541 tcgacattcg tcattccttg gctgttcaat tcgtcgcgac tactgcgcga ggacggggat
     3601 ctccgtcacc cgcaggcgcg tattgccgta ggggatgagt tcaacgtcag ccaaaggctg
     3661 gtccgacgtc accggactga cgggcggatc gtcggccgag ttgtccacca tgccccactg
     3721 ggggatcagc cgccccttgg ccttgaggac cacaggcggg ttgcgctgat caaacggcaa
     3781 cttgctgatc gagcgttttt cgacggcgat cgacttttcc ggattctcgc gatcgaccag
     3841 caggccgtag ttccaggggg tcgtcggctc gatcgaccag tcgattgccg gcagcgtctc
     3901 gtgatgccgc ttaatctcct tatactgctc gccgatcttg agcgagaagt acagcggccc
     3961 ccggaggatc gaaaccgcgt tgttgtagcg agtctcggtt ctcaggttca tcggcaaggt
     4021 cacttcaagc atgtcgccct gtttccagag tcgatcgaca acggcaaatg accccggccg
     4081 cgtgtcgacg gtctgttctg gagtcttgat ccgggcgccg gcagcccatg ccgggatccg
     4141 caggtgtagc gggaacttcg cgggttgcgg cgaatccatg cggatgcgga ccaagccgtc
     4201 gaacgggtag tccgtcagct cgacgagcgt cacctcgacc ccctcgccga ccttcgcttt
     4261 cacgatgctg gggccgtagg cgatgaccgc aagaccctgg tcgtgcgttg ccatccacag
     4321 gtgagccacg aattttggcc agccctggtg catgttcgcg gtacagcaac cgaagttcgg
     4381 ttccagcccg tagacgttcg atgcgtcgcc gttcgtactc cagcggcgtt tggccacgct
     4441 gaccagcacc tggttggcct gctggtcgta ctgatgggcc cagtaatccg gtgtgcacgt
     4501 gccgggattg gcactgtagg ccagcatttc caggcggtcg gcatagtaca catctcccag
     4561 ttgggccgcg agattttcca gggagaacat gaactcgacc acggcgcata gctcggtgcc
     4621 ttgtgtcggg cgtcggccgg aaagatgttc atcgccggca aagcgcccgg tcacctgccc
     4681 gtggtatttg tcgaggctgg ccaagccggt tttgacggct tctaccagtc tcgggtcgtg
     4741 agcctgaatg taccgcatac cggggtgctt gacggccatc gcaatgttca ccacgtgggt
     4801 ggggtggccg tacttgacac ccatggccat gacgtcatcg gtgtacggga agttgagaaa
     4861 gtagtcggtc cacttgaagc tgttgttgta gatcgagtcg gccacttcca ggatggtctt
     4921 gtcgccggtg cggttgtaga gccaataggc cgtgatgatg ttttccatcg cccggacgcc
     4981 acgccactcc ttgtcgggcc agtctggggg ggccgccttc aggtagttga agtagttctg
     5041 cagcaagggg atgacgcgcg ggtcgccggt ggcctcctgg tattggatca acaccttcat
     5101 cgccaccgcc aggggccagc gatcggtgtt cttctcaggg ccgaaccatc cgtccggtct
     5161 ggccgaggcg atgatcggct cgatccacga cttgaccttg gcgatcaaac gctggtcgtt
     5221 gagcagatac gccaggggaa ccaaaccgtc gaggtagtaa ggccctcgct cccaggcttc
     5281 tgcatccttg tccttgctgc ctctccaggc gttgttggcc aggctgggcc agaactcatc
     5341 gaggcggccg gtcaatccgt tggcctggac agtcagttga tctttgagcc aaccttcggg
     5401 cctgacggtt cccaagggta ggggcacaaa cttgctttga agcagcggtt cacggttgac
     5461 cggatagtgc ttgttggccg gctcggccgc cagcccgctg gtcaccagta atacgattac
     5521 acacagtcca atcaccaacg attgcatcat ggtcattact ccgtcacagc gggggcaagg
     5581 aacacgcaaa cgaagatgac tatatactgc caactgcgtc ccattgccat gtgtcatacg
     5641 agaggcgcgt ggacgggcag tccatcagac aggcgaagcc gccgctcata aatggtttcg
     5701 cggtaatggt taacgggccg agcggatcat gagggagtgc tgacgggggc gtcggcgccg
     5761 cttgctacac ctccagcttc catggtgccc gatacggata ccggagcagg tcgtccagat
     5821 cggttcggcc gaccggtcgc tcgttcttgg cgtcccagat gatcgtggac ctgctccgca
     5881 gggccaggtt gcccaagtgg gcggcggtgc tgatcgcgtg gccgagttcg aaatcctcga
     5941 ccgggcgctg ccgcgatcgc atgcaatcca ggaagttgcg aacgtgctgc ggacggccgg
     6001 cctccgcgaa tttgctctcg tgcttctcgg cggggagagg ttcttcctcg gatggcaccc
     6061 gcttgtcctt caccgcaggc gcgacttccc agccgtaagc cccgacagtc agcgttccct
     6121 cggtgccgta gaatgcgatg ccgtgctcct tgccgtcgac gccgcagcct gcggtcatct
     6181 ggtgctccca tatcagggca aagccggggt attcgtacac ggcgaactgc gtgtcgggcg
     6241 tatcggagat gtcgtcgtag atgtacttgc ctccggacga ggagacgcgc tcgggcatcc
     6301 cgaccttcat gccccagagg gcgatgttga tcagatgcac gccccagtcg gtcatcaggc
     6361 cgccggcgta atcccagaac cagcggaagc tgaaatggaa tcttgccggg ttgaacggcc
     6421 gtttgggcgc cggcccgagc cacatgtcat aatcgacacc gtccggggcc ttgtcttggt
     6481 cggccggctt cccgacgctg tggacccagt tcagataggc ccagcagcgg accatgcgta
     6541 tcttacccag cttgccggaa tgaacgtacg caatcgcctc ggtgaagtcc gggttgctgc
     6601 gccactgggt gcccatctgc accacgcggt tgtacttgcg ggcggcattc agcatcgccc
     6661 ggccctcgcc gacgctggtg gccaagggct tctcacagta gacgtccttg ccggcctggc
     6721 aagccatgat cgtcggaatg gcgtgccagt ggtcgggcgt ggccacgacg acggcgtcaa
     6781 cgtccttgcg gtcgatgacc gtgcggaagt ccttcaccgt ctcgggggcc ttgccgcggg
     6841 ctttctcgat ctccttgacg ccctcggcta gcatcttgtc gtccacgtcg cagatgatcg
     6901 ggcactcgac gtcgccatat ttgagaaaag tcagaatgtc gccccggccc atcccgccgc
     6961 tgccgatcag gccgacgcgg atcttctcgt tggcccccag cacggaccgg ggggcggcca
     7021 atgatgacca gacggccgag ctggcggccg cggccatgga actgttgata aagtgacgac
     7081 gagtgatgcg ggacatggaa gatctcctaa aagaagctgt cagccgtcag ctttcagcgg
     7141 tcagcttgag aaccaagatt gcgatatctg cccgaatacc gtactcggcc gcctggacga
     7201 ccgcaacagc agaacctcaa gcctcggcgg gcgtagcgca tccggctcgc agatgatctc
     7261 gcgtccttct tcggctgatg gttgaacaag ttggctcggg gctcttctgc agcgtcgagg
     7321 cattcagtga ttcaagtcga gtagcgcgca tcgccttgtc gcgaaagccc tgtctgtggt
     7381 atgaatgcat ccatgagagg caaacaaatc atcagcgtcc tcattccagt tgtctcactc
     7441 tggccctgga ttgccctggc cgagtttccc ggcgagcggc cttacccggg aatcacatac
     7501 gtgcaggaaa cccgtcagga tccgcccatg cgcctgttta ttgctcaggt ggatttggcc
     7561 gacccgaaag tgcgtgtcga tgttgccccg ggcgggcctg acccggacgg gcccggcgag
     7621 tgggagacga cgctgatggt tccaagcaag gttgcgtccc gcgagcgatt cgacctggcg
     7681 gtcaacggcg atttcttcag cattcccaag cccgccagct ccgcagctca gccgtcatat
     7741 cgccccgagg tctgggccaa cgcgatcggg ccggcggtga cggacggaaa ggcttggtcc
     7801 gttgccgaga agaagacgcc ctgcctgatc gttcgtaaga acggacgtgt ttcgatcgag
     7861 atgatcgacc ggccaccgtc tgacgcggcc gaggttgtcg caggcaacgt gatgctggtt
     7921 gagaacggca agccggtgcg gcacgagagc aaagccagac atccccgaac ggttgtcggt
     7981 ctggacaaga aaaaaaaccg gttaaccatc ctggtggtcg atggtcgccg gccgggcgcg
     8041 tcgatcggca tgagctacga ggaactcgcc aaggaaatga tccgccttgg ctgccacacg
     8101 gcggttaatc tcgacggcgg cggcagttcg gtcatggtca tgcgcgatcc ggaaaccaac
     8161 gagtataaga tcctcaataa acccagtgac gggcgggaaa ggccggtcgc caacgtgctg
     8221 gggattaccg tcgggaatgg ggagaagaag tagtcagtta tcagttctca gtgatcagtc
     8281 gagcgacttg cgtcttgcct tgccgacagc tacatgctga tggctgaccg ctctcctcag
     8341 tgccccggca acaactcgat cagttggtcg atttcctcga gcgtgttgta agcatgtggg
     8401 ctggcgcgca gacggccttc acggcaggaa atgatgacgc ggtgttgctc ctgcagacgc
     8461 tttcggatct ctccatggtc gtggtggtct gagacgaatg cgacgatgct gctggcctca
     8521 ccggggagtc tggatgaaac cacccggtag cccttgcccc tcacgccttc gaccagccga
     8581 tcggtcagcg cgagcgccca ttgggccacg acatcgatcc ctgcttcgag ccacatctca
     8641 atcgacgcgc ccaggccgca gatgcccgcg agattgtaac tgccggtgtc gaacttgcgg
     8701 gccgatcgca ggaactcaaa acggtagttg ccgaagtcta gcgcgtcctc catgcacaac
     8761 caaccggcaa aaaccggcgt cagcctgtca agcagttcgc gccggcagta gaagatgccg
     8821 caaccctcgg gcccgcacaa ccacttgtgc ccgtcggccg agaggaaatc gatttgcatc
     8881 gctcgaacat cgatcggcaa gactcccagg gcctggatcg cgtccacgca gagaagaacg
     8941 tctcgctcac ggcaaacctg ccccaggcgt ggcagatcca tgcgaaatcc gctggcgtat
     9001 tggacggctg agacgctcac cacgcgcgtc cgctcatcga tggccgcgat gacgtcctcc
     9061 gccggcacgc gtccgtcgac ctccttcacc attcgcagat cgacgccctg cctgccgagc
     9121 cccatccatg ggtaaacgtt ggccggaaac tccaccgccg tgatcacgac gttgtctccg
     9181 gcccgccagg gcaacccgcc ggccacccag ccgatgcctt cggtggtgtt tttcgtgaag
     9241 gtgacctcat cgggatcggc accgatcaag gcggcggcga gggagcgggt ttgctcggca
     9301 ttcatataga aatccgcgcc gatcgtggcc tgcccgcaag tatgttcgat gtagcggcac
     9361 atggcctggg acgccggact gctgagcggg gccaccgccg cggcgttcag aaagttgtac
     9421 cttcgcgtta tcggaaacag ttctcggagt tggcgcacat tcattcgttg gcaatctccc
     9481 gtgcttccac cgttatcggc acgtccgatc atctcttgac ggccaagacc gataggtaaa
     9541 ggttccctga aaacaaggaa agcaacatca tatcagacgt cctgtttctc cgctcgcgcc
     9601 aatttgcctg aattgcctgt cttctttgcc cggtggggca actgaatcgt aagttttccc
     9661 tgcgagcaag ttgctcacgg ggaaagcgag ccacctccgc accgcggcgg gtctggccgc
     9721 ggaaccaaag agtgctttga cgacagcttt cgcacgttgc aaggggggac aaccgtgtcg
     9781 cgctctgcct cattcattcg gatcgccata ctctcatccg tcgtgccggc atgcctggtc
     9841 ggatgcctgc cacttgattt cagcggctgg ggagatcaag ttctgtcatt ccccgcacca
     9901 caaccgcttt ccggaccgca aatcaatgag gtcgaaccca acgacgacat ggctgcggcc
     9961 aatgcagttg ttttcgatgg ccaggtgacg ctggtcggaa gcatagcggc caagggcagc
    10021 ttcgacttcg acgtctttgc gcttggaccg gtgaacgccg gtgaccgggt ggttgccgag
    10081 ctcgctgcca gcgccggcag tgacgtcgtg ctcgggctgt ttgacggcgg cttcaacctg
    10141 ctgggctacg tggattactc gcggggaacc gcggctcccg gacgcatcga cctggttctg
    10201 cgcgactcgg tcgacacgct ttatgccatc gccggcacgc gttccttatc ggaagcgacc
    10261 cgactgtaca atgtcggcat cagtgtcaca ccgaacgtgg gtgttccgca gcccaatccg
    10321 cagatggtag tgctgaactt catcggggcc tccggagttc gaatcggacg ccaggcaccg
    10381 gtcaacgtgc cgccgtttga tgtggccacc attgattcgc gcttcgccgg aaagacccag
    10441 gaggtcatcc agtccatcct gcaaatggtc cgcgaggact atgcaggcct caacgtcgtc
    10501 ttctacctgg ccggtgatcc gaacatcccg gccggcgacc tgaccgttat tcactttggc
    10561 acctacagcg accgctatct cgggctggcc gacaacgtcg atccgttcaa tcgagacagc
    10621 acgcaaaacg cgatcattta cactgacacg ttctcgctgt tcagcacctt gtggccgacg
    10681 caggagcaga ttgcccaggt attggcgaac gtggccagtc acgaactcgg ccatctcctt
    10741 ggcctgcggc acactgctga cattcaggac ctgatggaca tcaccgcgag cgcccggcaa
    10801 atgcttagcg accagtggca caaggtggcc gatctgcatc cctcgatcat ggcgatcgga
    10861 taccaggatg ccgccacgat gctgtcttgg tgcgtgggcg gcacaccgcc ggcacctcct
    10921 gcgaacttgt cggccaagac acaacggatg gccaaaacgg cctacgaccc ggacgatttc
    10981 tacattccac gagggtggct ggcagagaac tccgctgagg ctgatgaagc cgtcgaggat
    11041 ccggccgagg agtagctgac caaagccggc ggctgcccgg ggggtcaact cttcatgcag
    11101 cttggatcaa ccgatacgcc cctgccgctg atgcactgcc ggaacagggc aaagtcgctt
    11161 tcgtccacgt cgttgtcgcc gtcgaacttg gcgttctggc atctgggctc gttttgcgac
    11221 gtgccggacc cggacagaca tgactgaaag tgtccgaaat cctcctgatc gacgtcgccg
    11281 tcttggtcga aatcggcctg gaccggcctc ggcccctcga cagtcagcga tgccgcgttg
    11341 gacgctgcgc tgccctccga attgctcacg acacagcggt agaagccgac gtccgaccca
    11401 tccgcgttcg acacagtcag cagagcagtc gtgacgcctg agcaatggcc tgcctcggcc
    11461 aagtcaatca aatccttctg ccactggtaa cttacgggcg gaaagccgct tgcggtcacg
    11521 ctgaacacgg cggtctcacc caagtagacg gtttgcgaaa cggggtgttg gctgatcacc
    11581 ggtggttcga ccaccggacc gatggtcacg ttgtccacga gggtcttggc tccgctgatg
    11641 ctactccggt aattgtaaat gaacggccgg cccttcaaca gcgagctatc atggatcggg
    11701 gtcgcggtca acagttggtc gtcgacccaa acgctcagtt cctgtccctc catggaaagc
    11761 cgcatcttgt gccagccggc tacgtccggg taggtccagt ttgccagcac ggtttcgatt
    11821 tcgttctgcc gccttataag cacacagtgg ccgtcggccg ccaggttgtc attgcgcaca
    11881 aatgcgagcg cgtagtagct acgctcggcc gcggagaaga acaggcggcc gcgcaggcca
    11941 acgccgaacc ggttcttcac ggatgtgctg ttgtcgtagt atacctgggc ttcaatgctc
    12001 tgattggtga gcgcctcatc ggccagcatc gggtaggcgt actggtggcc gctggtgtca
    12061 gcgacctcga atacatatcc atccccgctc gggttgccgg gacgggcaac gagcccgcac
    12121 tgactcccga agttccacgg ggcgatccag ttcagttgtg ggccggcggc cggaaaggca
    12181 tcctgccagg gcatcgcgat ggccggcttg tcgatctgga aaccgtccag aacggtccca
    12241 tctgaggtct tgaggcaccg gccaagcagg cggctgccct ggatatcgag gcgtgtgaag
    12301 cagtaatact ggtccgcgta cgcccgaacg tagggctggc tgtcatcggc atgcttcgtc
    12361 gaccacggct ggccaccggt accctgatcg atgtagcaca cgccgttgac gacgttgcgc
    12421 tgatagttgt ggtagtggcc gtgcagataa agatcgacac cgtgctgctc gaaaagcggt
    12481 accaggctgg ttcgcatcgt cgtgctgccg ttcgagcctc cgtcctcgct gacgtacggg
    12541 ccgttgtgcc cgaagacgat cttccatggc tgggtggtac cggtgaggtc gccggccagc
    12601 cagttgcttt gcgccgaacc cacaccgtag gaggaccaga acgtgtcgat gctcgcgaaa
    12661 tgcacatggg ctttgtcgaa cgagtagtag aattcggtgc ccgaactagc ctttgggagc
    12721 gaccaattgg ccaggtaccg ggcgggatac acgtaggtgg ccgtttcatc gtacagctcg
    12781 tgatttccaa tggctgtata catgcaaatg tgtttgatga agccggccgc cggcgtgaag
    12841 aagtgcgatt gccagtagtc ggtgatgttg tcctcaccgc gacctgccag atcaccgaga
    12901 gtcaagcaga acccgttagg cgattgcgac agcattgcat tataggttgc cgcgcagttg
    12961 ctcggggcgc tgcacgaatc ccccacaaaa ctgaacgaga cagccgaggc accgggcggc
    13021 ggggcggtcc tgaaagtggc atcaccgctg aggggcgtgc cgtcgctgcg agcgcggtag
    13081 tggtagagcg tgtccgggga caggcccgtc accgcgattt cgtgataggt gccgggctga
    13141 gttgattggg caagattacc gtagctcgcg gtcaagcccc actcgacttc gccggtgcag
    13201 gtgctatccg tgaaccacac cacgctgacc gagtccgggg tctggagttg aagatagggc
    13261 ccacgagtga tttgcacgcc ccacgccggc agcgccgccc aggcgcacaa tacggcgaag
    13321 gtaatcagaa gtcgccgccc tatccttttt tggggagcca tttggactga cctcgcctga
    13381 ttggctggcc cggccggacc gccgggtcgc acatgaccat tgtacagcat aactcccccc
    13441 ggtgatcgcg ccttccgtgt tcttctcggg gcagttccgt gcgcagtccg cggcgaatca
    13501 tcttgagtca gagatcgttc ttgctctctc ggccgtgatg gtctcagtgg cctgggcgcg
    13561 caaagcgcgg attgtcatgt ccccaacccg gcgggttccc tcagaatcgg atctggctgg
    13621 catgggttat atcgagatcc ggaggcccga tatccgcggc ccccatgtcg gcccatccgg
    13681 ggagaatcgg caagatagct ctcgtctggt tgcctcctcg ccctcggccg gttaaactgc
    13741 acggagaacg catgtcgcag gaagggctcg tgggtgctca gggtcgagcg gtcaaaaccg
    13801 gcgggcggtc ggttacgtaa tatcagcgca tcgtttgggg acatgcccgt cgcaacggaa
    13861 gtgaggtcgc catatgccaa gcggaagtcg tacaggtcag ccaccaagct ctcccgttca
    13921 tgattcgagt gcgccgacgt atatgccgcc ggcgggaggg ggcagtcctc cgtccagtca
    13981 gtcttcatcg gttgaatcgc caggacaaaa ggccccgacc gtcggccccg gtggggggct
    14041 ggtcgacctc cacatcaccg agcagaatgc ccgggaggtc atggaccgtc tgctgagagg
    14101 catggggcaa tccagcggtg agggtggcca ggcgcatgct cgtgcgaccg atctccacat
    14161 caatcccggg cgaccgccgg actaccgtat cgcccgggtg attcgcagcg tggccgctcg
    14221 tcccctgagc caaaaggatg tgattctcct gtccaatgcc atcatgaacc cggcccagca
    14281 ggagcggttc aatcgcaacc actgcatcga cttctcctat cacgtggacc gaaaccgttt
    14341 tcgcgttaac atcgtcgaga gtttcattgg acgaagcatt tccatccggc ggttcgagga
    14401 gatcaagaac ttcgaagatt acaaccttcc cagcaactac atcgatattt gcgagaatgc
    14461 ccgggggttc gttatcatgt ccggctcaac gggcagcggc aagaccacct ccatcgccgc
    14521 gatgctcgac catgtcaacc ggaactttca ccgcaagatc gtgaccattg aggacccgat
    14581 cgagtacgtt tacaccccga agaagtcgat cgtagtccag atcgagatcg gcgaggacgg
    14641 gatcccgaat gaggacattg ccttgcgcaa tctggtccgt atggacccgg atgcgggcct
    14701 tgccggcgag atgcgtgatc gacagtcgct ggaggcggcg ttgaagaccg cccaagccgg
    14761 tcacctgatt ttcggcacgc tgcactgcga aggcgtcggg caggccttcg accgcgttct
    14821 ggcatattac gataccatcg agcagcaacg catcctgggc acgctggccg acaacctcgc
    14881 ggcaatcatc aaccaacggc tgttaccgtg catcaagccg ggcatcgacg tggtgccggc
    14941 ctacgagatc ttccggccca acccgctgac ccgccggtac atccgtgaac ggcggtacac
    15001 agaagcggtc gctgaaatgg agaagcagtc catgaaggaa ctcggttgtg tgacctacgt
    15061 ggactcgctg tacaagctgg taaccgagga atggatcgat taccacaccg ccctcgaaca
    15121 ctccggcgat caggccgaga agctcaaagc caaactgaag ggcattgata tgaaatgatc
    15181 gggggcgtca tcgtcccacg ctctgcaccc cttggcggca agtcgccgtc acgtgaccga
    15241 gcggttcaat aaccgatccc ggccgatcgt tcgaggggag gaactacatg cgtctttttc
    15301 gcttggcggc tatccatgcc cgcaatcggg ctaccggcaa cgtattgagg atatgctcgg
    15361 gcgtcgccca gcctcgacgt gcggtttgaa cccccaatgg catctggtcg agctgatcgg
    15421 tcgaatgggc atccgtgctg atcgccaggc ggcaccccgc atcgatcgcc tgccgaatat
    15481 gaacgtcttt caggtccaga cgctgccacg aggcactgat ctcgagggcg cagcccgtcc
    15541 gtgcggcggt ggcgaagagc tcatcccaat caagatccat ggcatcgcgc cggccgagca
    15601 gacgacccga cggatgcccg agacagcaaa cgtacgggtt ctccatggcc gcgatcgagc
    15661 gacgggtgag gctggcgcga tcctgctgct gggccgagtg aatactggcg atcacccagt
    15721 cgagctgagc aagaatgtca tccggatagt cgaggctccc gttggccagg atatcgcact
    15781 cgatgcccgc cagcagcgtg aggcccttga cccgctcgcc cgcggcccgg atgtcctgaa
    15841 tatgccgcag caggcgggtc tcatcgagac cgttggcgat caccgagctt tttgagtgat
    15901 cgcagatcgc caggtaacgg tagcctcgct tgccggccgc ctcggccatc tcctcgatgg
    15961 tgcttcgccc gtcgctggca gtcgagtgaa cgtgcatgtc acccttgatg tccgccaagg
    16021 atacgagccg gggaatctcg cctctgcact cgatctcccc gcgatcctcg cgcaattccg
    16081 gcggtatcgg gcgcagatca agcttctcgt agatctcctc ctcttcccgt ccggcgatcc
    16141 ggcgatcccc atcaaagagg ccgtattcgt taagtttcca gcctctccgg acggccagtt
    16201 cccgcaaacg cacgttgtgc tccttcgatc cggtgaagta ctgcagcgcc gctccgaatg
    16261 actcctcggg cacgacgcga aggtcgacct gaatttctcc gcggacagga ccggcgacca
    16321 gcacggaacc cttcgtcccg ccggccgcac gaacgccggt cacctcgggc agcttggtga
    16381 acgcgttgac caccctctcg ccgtcgtcgg cgatgcacaa caggtcgatg tcccccaccg
    16441 tctcacagcc tcgtcgtgcc gacccggcaa tctcgatgcg ccggacgccc gtgaattcgc
    16501 gcaccgcatc ccgaagcccc tcggcaagcg gcagcgcggc acccaacggc gtgcgacctg
    16561 ccgaacgttt gagaaacgcc aacccctcgg cgatcttttc gacgctcttc ttgccgaatc
    16621 cgggcagcct ctccgcgcgg ccgtcggcaa tgaccgcctc caggtccgcc atgctgttga
    16681 cgcccagctc gcggtgtagg gcctgcacct tcttgggccc cagccccgga acggtgagca
    16741 gctcgggcag tccccgcgga atcgaattca gcagttcctg gtggacggcc accttgcctg
    16801 tcttcaggta ctgcataatg cgctcggctg tccccttgcc gacgccgggg atgttctgca
    16861 actcgccgcg attcgccacg tcggcgatgt cctccgtcaa gtcctcgatg gagcgagccg
    16921 cttttctgta tgaattgacc cggaacgggt cgccgccggt cagttccagc aaatcggcaa
    16981 tctcgttgaa aacagccgct atctccgggt taaccatgtt ctttttccat tatcctgcga
    17041 gcctgcgtcc gtgcgccgat ccaacgtgcg attgacaccc gctgcccgag gaatgatatt
    17101 atagcggtct cgaacgactg gtgggcagtc gctagacagt gagcattgaa tgggcctgac
    17161 cctgacgcaa ttgaccgagt tcctcgcatc tcagagtatc ccttgtgaag tggacggaga
    17221 cggctctgtg gttgtctcgt cagtggctac tctggaggac gccggcgagg gacagctcag
    17281 cttcttgtca aaccccaagt acgagaaaga actggcggcg acaagggcct cagccgtgct
    17341 ggttcgccct gacgtcagag tgtcacgcac gatgaacctt cttcggacac cggaccctta
    17401 tgcggcgctg acggcggcga tcgtccggat ccacggctat cggcaacacc cgcaatgggg
    17461 ccgctcggcc caggcctgca ttcacccctc ggcggtcatc ggcgagaagc cgaacattgc
    17521 gccgggcgtc cacatcgatg agaacgtgcg gattggccgt catgcgacca tttatccggg
    17581 cgtttacatt gcccgtggct gcaggatcgg cgatgacgtg gtgctgatgc ccaacgtggt
    17641 catctatgag gcctgcgtgc tcggcaaccg ggtcaccatt catgcgggct cgatcatcgg
    17701 tgaggatgga ctgggctacg ccccggtcgg cgaaaaatgg gtcaagatcc cgcagatcgg
    17761 caacgtggtc atcggagacg acgtggaaat cggagccaac tgcactattg accgggccac
    17821 gctcggcagt tcggtcattg gcagcggcac caagttcagc aacctgatcg ccatcggaca
    17881 cggcaccaag atcggcgagg attgcctgtt tgtcgctcag gtcggcattg ccggttcggt
    17941 cagcgtcggg cgtcacgtga ccatggctgg acaggccggc gtggtggggc atatcagcat
    18001 cggcgacaac gcggtcatcg gagccaaagc cggcgtgacc aacgatgtgg aaccaggcct
    18061 gaccatgttg gggcaaccgg ccgtccccat caacgagtgc aaacggcagg tggcgatcgt
    18121 gcagcgactt cctcaactca aaaacgagat caagcgcctg cggcgcgacc tggatcaact
    18181 catgaagcag gtcggcggag gatgagcgat catctgtaca atcctttggg catcatcgcc
    18241 ggcgcggggc ggtttccatt cctcgctgcc cagggtgctc accaggccgg ccgagcggtt
    18301 gtgatcgtcg ccctcagagg cctggccaat ccggagctcc ggacagtggc cgaccggttc
    18361 cactggtcgg gaatcgtgcg gctcggccgg tggatccgcc tgtttcgcaa gtcgggcgta
    18421 aatgaggcga tcatggccgg gtccgtgata aaatcggaga tgtacggacg gctccgcatt
    18481 ctgcgatacg tacccgattg gaccagcatc cggctgtggt tcttcgaggt ggccgatcgg
    18541 cggaacgaca ccatcctccg tgcggtcgcc gagcatatgc agcgggaggg catcactttg
    18601 accgattccc tgcaatactg tcgcaagcac ctgcctgcgc ccggcgtgct taccaggaac
    18661 cggccctcga aagcgctgga caaggacatc gagttcgggt ggcacatcgc caaggagatg
    18721 ggccgactgg acatcggaca gtcgatcgcc gtcaaggaac aggaagtcat cgcggtggag
    18781 gcgatcgaag gcaccgatcg catgatcgag cgggcgggcg aactctgccg tcacggcggt
    18841 tggacgctga tcaaggtcgc caaacctaac caggatctcc gcttcgacgt gccgaccgtc
    18901 gggccaaaca cgatcgagaa cctgcatcgt cacggcgcca aagcgctggt tttcgaagcc
    18961 ggagtcacgc tggccatcga cgtgaatcag atgatcgccc tggcagaccg atatggcatg
    19021 atcgtcgttg cccatgagcc gggcaacgca actcacacgc aacagaccgc gtgaggatgt
    19081 ctggcgaagc tgatcgacgc tgcaggagaa cccgccacaa ccggccggcc ttgcaaaaag
    19141 accgtcagaa gccgatcttc agataattgc aggttggacg ccccagagca gcatcgcggc
    19201 cgggtttcag taggcatgca caagtccgag ccccgcgcgg ggagggtgac gccccctcag
    19261 caaagggcgg cgtacatcag gaccgtcaag agaatgatct ttgtcttacg agcgagaaac
    19321 atgtagacca tcgtggcgta ggcgtacatc tcttcttctg cctcctctac ctccaccggc
    19381 ctcgctctcg gttccgggcc ggtctgctaa gctgaggaag caaccaatgt tccgaggtgt
    19441 gccagagcgt tctcttcgat cagtatggca taagtcttta tgccacaatg ctttgtgagc
    19501 cgaaaagtga tgttgttaaa atgggtgatt tgtgcggctc gtgagggctg ttggaatgcc
    19561 tcgtcgacac tcagtcctga gcaggtagta acttcgcgca agtccttgca catccagtag
    19621 taacacctat gatgccggcc gccgacgtct gcggagcttc cggcaggcgt ccgcttctgg
    19681 acagcgctgt tatcgctctc gccggttcgg aggacgggta tgcacagccc ggacgagctg
    19741 attgccttgc ttagagaaga gattcgccag cggcgcgaag agggatgcaa tgtagtgggg
    19801 cacgctcaac ggatgcgaaa ggcaggcaag cgcatcgatg agctcaacgc gctctacgac
    19861 gacctgatga ccttgaaacc cgaaccaaga ttggcgaggc gcgagccgaa cgacctcgcg
    19921 gggattcggc ggcttcggcc gcgcagacca cggcgaatca agtcgccggc ctcaaccgcg
    19981 cgattgcgcg atcggctccg aggagctttt ctcggacgct gtgccggctg catgctgggt
    20041 aaacccgtgg aagactggca ccgtgaccga atcttcaagg ccctgaaaaa agctgatcgc
    20101 ttcccgttgg cctactactt cgagtcgtcg acgatcgagg ccggaggtaa accgatccat
    20161 ccggccgggg taacatactg caccctcggt cacttcaagc acatggaacg cgatgacgat
    20221 accgactaca ccatactcgg cattcactgc ctcaagacct acggaccgaa gttcaccacc
    20281 gccaacgtcg cccacgagtg gcagatgaga ttgccctatt tcatgaccta tacggccgag
    20341 cgggcggcgt atcgcaatct cgtcaacaga ctgccgctcg atcaggttcc gctctaccgt
    20401 aaccccttcc gcgagtggat cggggcacag atccgggccg acggcttcgg ctactgtgcc
    20461 gccggcatgc cggagctggc ggcggagttc gcctaccgcg acgcgacgtt atcgcacgcg
    20521 aaaaacgggg tttacggcga gatgcttttt gcggctatga tcgccgcagc cgcggtgtgc
    20581 gacgacgtgc gtgaggtgat cgagatcggc ctgtcagaaa tcccggctgg ttgccgattg
    20641 gcgacagcgg tccgtgacgt caccaggtgg gccgacgaaa accgcaactg gcaggacacc
    20701 ctgcacgcga tccatgaccg atatggccac taccactcgg tccacaccat caacaacgcc
    20761 tgcctggtga tcatggggtt gctgtatggg cagatggatt tcggcaagac gatctgcata
    20821 acggtgatgg gcggctggga cactgattgc acgggggcca ccgccggttc ggtcctcggt
    20881 gccatgctgg gagccgaccg tctgcctcgc agatggatca agccgctgaa caatcgcctt
    20941 gagtcaatcg tgatcggata caacgatgtg aggatcaccg atatcgccga tgcggcgttc
    21001 gaggtaaaca gaaagatcag gcttggtggt ccgcgattca gcaaaggcta aggcggagcg
    21061 cctttttgcg ggtcgcaacc caagcgctct gcgatccaga aacatgcgcg caagccgcac
    21121 agatgtccgg ccataaggtt cgaaagacaa gcccttccgt gagaccaaag ccgataaggg
    21181 acaatcggat ggaaccccgg agacaacctt cttcgcaggc ttcttggctt gcactggcgc
    21241 accaccgccg gtgcacgtcg tcgatcgcgt tagagccgac cccgttctca attctggatg
    21301 ggccattcgc cattccccag cgccgccgtg tgcaatgctg gtgtgaacgc ggctttcccg
    21361 accgcgcagc cttgcctgac aatagtcctg gcctggcgca aggcttgcca gagccgacct
    21421 gtgtgtctgt ttcttcctga caagatatgc tgccaaattg ggcatgccag aatgaccctt
    21481 tccgactccc gatatcacct tggcgagtgc aactcttttg caaatcatga tttacgtaaa
    21541 ggcatctacc cagagcaccg ctggtacggt acttgccatt gtttggtgga aacaaccgtt
    21601 agcacaccgg tgtcgtatca agggtggttg gcgggatctg atggttggcg cggaggcggc
    21661 gaacgccgcg tcgcaagcgg taccgtactg ccgttgcccg gacctccgcc ggaatgttgc
    21721 gacccgtcgt cgcgaaagga gaagagccat gcaggtgcgt cgatacaacc gaatctcgcc
    21781 ctttgaacaa ctgcggcgcg agatcgccgg actgctggat gaatttgacc ccctgggtgt
    21841 tcgcggggcg ctgttcggat cgccaggttt tcctgcgatc aacgtctggg aaagcggcga
    21901 ttgcctttac gccgaagccg aaatacccgg cgttactcag gaggatctcg agatccttgt
    21961 tgtcggtaac gagctgacca tcaagggtcg ccgcggcacg gccgaggaag aagacgcgtc
    22021 ttaccaccgg cgcgagcgcg gtacgggcga gttctcgcga acagtgaccc tgccggtcga
    22081 tatcaaggcg gaggccgtcg atgcgacgct caaggatggg gtcttgacac tcagcctgcc
    22141 caaggccgac gaggctcggc cccgaaagat cacggtcaag gaagtctagg ccgtctttca
    22201 caaggctcgt gcgggaccgg catctcaccg gtctttgcag caggccggaa gcccgtcgtg
    22261 caggttcttg ggagcgcttg gacggcggac gagcagctcc cggcatcgta agcaggaaca
    22321 ggaaaacaat aacggagctc atgtgtcatg agaaagcaca aagcgatcgt gagaacagag
    22381 ggtgatgtgg cgcaggccga gcagacccgt gatcggcagg tctactcgcc gcaggtggac
    22441 atcatcgaaa aagacaacgg cctgttgctg ttggcggaca tgccgggtgt caaggccgcc
    22501 gatatcgaca tccaatatga ggacggtcaa ttgaccattt acggcaaagt ggagccgcgg
    22561 caggatgatt ccaaaaccag ctatctcctt cacgagtacg gagtgggcga cttctatcgc
    22621 tcgttccgca tcggcgacgg cattgatgtg gacagtatcc gcgccgagct gaagaacggc
    22681 gtcatgaccc tgcatctgcc caagaccgag gcgttcaggc cgagaaagat cgccgtgcaa
    22741 gcgaagtgag tggttcgggc gagcggcagg cgcccgttct cccgctcatc gagcagactt
    22801 taccgagcct gccgcctggc cctttctgct ttccactggc gaattctgcg cggcagccac
    22861 cggagcaggc ggcgcgggcc ggagaactcg gtggcgagaa tagccagccc ggcgataatc
    22921 acggcgaacc ccgggccggg aatcagcggt atgctcaaac cgatgccggc cagcaccacc
    22981 gccgagccga caatggccac caggatgcgc caaatgagcc gccagatgcg acgcattcgg
    23041 gtcgctggta ccgtctgcgc cgaggaggcc tctccgcagt cggtgtcttc gtgccggcgg
    23101 cctgcgtcag gcggtagtcc ctgtaactcc gtcattcgtg gtccctttct gtcgcttgcc
    23161 tgctatgatc gaggccacca cgccgatcgt caggatcgtc acgatgatcg ccagcgagag
    23221 attaatgtcg aggcgaatgc cgaaggccac gatgagcatc ttcacaccga cgaacgccag
    23281 gatgatcacc agcgccagtt tgagatactc aaactcgcgc atcatgccgg ccagagcgaa
    23341 atagagagcc cgcaacccga gaatggccat gatattcgag gtgaacacga tgaaggtgtc
    23401 cgcggtcagg ccgatgatcg ccggaatcga gtcgatggca aacaacaggt cggtcgtctc
    23461 cacgacaaga agagccagaa gcaggggcgt aatggcccac cggccgttct gtcgcacgac
    23521 aaaacgcgct ccctgaaact gggacgtcac cgggtaaaag tgccggacca gccggacgag
    23581 aggattacga tcgggatcga tatgctcctc tttagaaaaa agcatcttga cagccgtcag
    23641 aatcaacaac aacccgaaga cgtaagtcat ccaatgaaga tgatgaacca cccaggttac
    23701 cccgccgatc atgatgccgc gcatgaccag cgccccgaga ataccccaga aaagcacgcg
    23761 gtgctgatgc tctgccttga taccgaagaa tgagaagatc accgccatga cgaaaaggtt
    23821 gtctacgctc agcgatttct cgaggatgta gccgccgaag tactccttgg cggcggtggc
    23881 cgccaccttg gcgtccgctt gggccaccgg catcgggtac ctggccggat cactggcgat
    23941 gcgctgtgcc gcaatgccca tccagtcgtg cttgtagatg tagtaaagcg cgacgttgaa
    24001 agagagcgcc accgcgatcc acatggccga ccagagcagc gcctgcttga gcccgatgac
    24061 caccgccctg cggtgcaaga cgcacaggtc aaaaacgagc atcaggacga caagggtgac
    24121 gaacccgatc cacaacaaca gcatgaaggc ttcctctggg gccggcggtt ctcaggactc
    24181 atggcgaatc ttcctgattc gcgggaatag cgtaacggga ttcgtgggcg ggcggaatag
    24241 gcggaagccg gcctccgttt gtcaggctcc gcgaggacgg ggccatcctg cgacgttgcc
    24301 tttcattcat ccggtttctg ggacgaccga cccaggcggc gaatctcatc gaaaaacggc
    24361 gttcgccctt gttcaacgca ttcggccgca tagaggtaca tcgccgcgga ccaggtttgc
    24421 cagtcgtggc cgcgaggtcg acctgtctgg gccttgatcc actcgttgaa cccgtagctt
    24481 accttggcct ttttcgccgg gcggacaagc tcggtcagta tggccagctt ctgagccgcc
    24541 agtcggctgc gacctgcggc gaccagcgcg gcaatgtaga agccgcagac gaacggccag
    24601 acgccgccgt tgtggtagtt ccccggccga ttgtaccgct ggtagcgcgg ccgccagtcc
    24661 tggtcttcgg gttgaatata cgggatcaga cagggcggca ggtccacggc cagttccccg
    24721 cgacggcgca agcggcgaca ttgcgtttcc gtccagttga tgatccgctg ggcccgggct
    24781 tgcggggcga tgccggtgag aatcgcaaga ctgttgccca gcagatcgaa ccgccggctg
    24841 tggtagacct taaacgacca gagcgcatag tagggctttc ccggaatgac gaaaccctcg
    24901 tgaacatagc gctgtcgttt tgagccgcga aggtcgggat gatcgatcag gcaacgaagc
    24961 aggtcggcct tgcgatgctg accgtgaagc cgaagacacg cgtacacaat cgtgttgacg
    25021 taaagcccgt agcccagcac ccactgctca tcacgccagt cagtagtcgg ttgttggtcg
    25081 accatgacca tgtcatccgc tccctggtac tccatccagc gaagggcttt catggccgcg
    25141 ttgcggagaa accgcttgtc gccggtgacc ttgcgataca atcccaaccc gaaaaggaac
    25201 agcggcgtgg tgtcacttgc gccgagatct ttcgggtcgt gagccaatga ggcgatatgc
    25261 ccatgcggcg tctgagtccg ggccagcgac agcagcaccc gccgcagcgc atctcgcagt
    25321 acggaatccc cggaggaaag aaagcccagc gcggaaatca tcatgtcacg ggtgtacggc
    25381 tcggggtaac cccacgcggc cgtccgcggt agcctctggc aaggcccccg aacgttgttc
    25441 aggagcacct ccatcgccgc cgccttggcc tcggcggtct gctccaggct tgccgacgta
    25501 cgactcattg gatacccaac ttctcacgga catagtcgac aaactggcgc gccaccaccc
    25561 ggtagtcgta cttgcttacc acgcgctccc gtcccttcgt tcccattcgg gcacgcaagt
    25621 cggcatcagt catcagagtc ttgagatgct cggcaatgtc gtagatgctg gcccggtagt
    25681 cgacgatacg caggttctcg aaaactaccc gctgcgcctt gttgaaaccc gactcttctc
    25741 ccagaatggc ctccgcgatt cggatttcct gtgccactcc cgcaagaagc gccgtctcac
    25801 cgtggaccaa cgtctcggcc ggggccatcg cattgatact gaccaccggg cgtgcgcacg
    25861 cacccgcctc cacctgcggc atcccgaagc cttccagtcg cgacggggcg gcgtatatgt
    25921 cacaggcggc gatgagataa ggcatgaagt tccgcgacac cttgttcgtc gtgtagatga
    25981 cgttcttctc gatacccagt tgcgtggcca actcccgatc catacggttc tgaatttcgg
    26041 ttcgctgctg cggccagacc ttgcaaacgt atttccactt ggggacgtcc ttggcgatca
    26101 aggccagggc ctgcatgacc tcctgcgcgc ccttcgacgc cgcgtccccg cccaccgtca
    26161 ggatcatcag atcatcgggc tcgacgccca gtgcctcccg cacgaccctg accttcgcat
    26221 cactgatgtc cctgggtatg aagctgtcgg tgtcgcagcc caccggcagc acgcacatgt
    26281 tctcgccgtt gataccgtcg cggatataga cgttttttac ccattcagag gtggtcagaa
    26341 tcaatgggag agagttgaga acctcgcggt agttggccac gaagccgtcc gccaccagcc
    26401 acggcaccgc ctggacacca tgcctttgcg gatggagaat cagatgcggc gtgtggcccc
    26461 aatacccgac acctaccact acgtccggct tgaaccaccg gtaataccat tctttctcct
    26521 gggccgactc gaaattcacc gggtaaacat cgacgcccag ttgctggagt ccccggtaaa
    26581 gcagatcccc ctgagtcgcc aacccgccgg gtgacggcgg gtagtcatac agaaccagtg
    26641 ctttcatcga caggatcctt cctcgcttcc aatccatcca ttcagcgcgt cgggagactc
    26701 aaaatgccaa accgcctctt gtcggggcgt tgcctgggcc tcccaactgt cgggcgcaaa
    26761 ggcgtaaagg ccggtgataa tatcgtattt ctgttgccat atctcttgcg agagcgtctc
    26821 aaccaggtgg gcatttgctc ggggtcgggg caggtatgcc cgttgcccgt cctcgaaggc
    26881 gaacaggaac aaacggccgg ccgtcagccg cccattccgc cataaacggg taaccgcctg
    26941 cgaagcctcc tcatgacgcc ggtgccgggt gtattccccc cacggcccgt gtgtcaggac
    27001 cgcgtcgaat gccgttccgc ataataacga caggatggcg tcctcaactt cagcccaagg
    27061 cagcggagtc tgctccggcc cgtcatccag gtcggccatg gccccacggg cgttcaggcg
    27121 ctctaacacc cgccggaact tgggagcccg gtccgggtca ttgccccgac acagagtcac
    27181 aatggtccaa tcccagtccg gtcgagccaa tatctggccg ccggcccaca gcgtttcgtc
    27241 gtcggggtgc gctacaatga ccgccaccct gggcggccgg taggaggaat caagaatagt
    27301 gtccagcagc atgcccctac ggctccagcc aggcgtctgc caagtcggca tggtcgccga
    27361 caatcccgtc atcggcatcg gtcacgatga tagtcagcgt cttggccccg gcaacagaga
    27421 cactgaactc tctggcgggc tcgtgacgcg ccatccgccc gctatcccag cgtttctcgc
    27481 cgtccacgtg aatctcaaca gtgatggtgc cgttcgcggc cgcgtcttgg cccgccaggc
    27541 cgtgaaaggc cttgtacttc ccgccaagat cgtagaccac ctcggccggt gcatgcgtgc
    27601 cgagcccacg gtggtagacc tgggagccga tgcacatcgt tttgtcccac acgctcttgt
    27661 ttttcataag cctgccccag ccctgcttga ccgaaaccgg ctccagccgg tcgaggtaga
    27721 tcccagggat cctcttgatc ctgggctcaa aggtaatgac ctccatatcc ggagtcagca
    27781 gtctcgtcgg agcaatcgca accaccgata tctgcgatct acagtcgggc ggcaggggaa
    27841 atggcggcat gcggccctta tgtggccccg gttgtgtgat ggctgccggc ggatcgaact
    27901 ctcggaccaa ccatgcggat gccgagatgt catgttgcgg cgtgttcatg gccgcagaca
    27961 cttgccattg tcctgatgga agatcaacca cgtaccactt ccatccgtgc tttgacttca
    28021 atcccgaggc gcaccactgg cccgccgagt cgatcagcgt gctttcagcg gccaagccgt
    28081 tgatcgtgaa cactggatcc aggacttccg gcgatttgtc cgattcaaga aggtagtaaa
    28141 ccttccagcc gcgcccaggg ctccgtcctt cgatcgtcga ggtccaccgc aagccttccg
    28201 gcggcgtcac gattgtgtag tcatcgccga agggttgcgt cccaggggat tgagcagtcg
    28261 tcttaaccgc cttgctcttc gccgaggcga cttttgcccc gcacacctgt ccgctggagg
    28321 agtcgggcag atcctccctg attgacggac aaaggcggat gaccttcgtc tcatacggtc
    28381 ccatgctcag cgtcagcctg tcgccatgat tcagtccgac ggccacgcaa cggcgatggg
    28441 ggtattcctg tattgcggag tatctctcga acctcacgtc cgcgccgatg gtcgcttcgt
    28501 caagcagcac ctcgaccaag tccatctgaa tccacgggtt gcgcaggcag atcaggccct
    28561 ggccgtcgtt ccaatgagca tatccgtagg tctcacgttg catcgccttg gtgtcgtgga
    28621 ctgtcggcaa gcccttctcc cgccacgttc gcggccagac gagtttcgtt cgctcaagca
    28681 acgacgcatt ggcccgcgac cacgtcatga gctcggcaag gaagcggtac tcgtccggcc
    28741 gcatgtgctt gggattgagg tacaggctga tgaactggtg cccacgcaga accgtgacca
    28801 cggcgtcgtt gcacagcggg tcgggcgtct ggtgcacgat gccgagcacc tcttgagcgg
    28861 caatcgggac gggcgttgcc gccccacgca gattgtaaaa gtcgcgggag gtcgtatagc
    28921 tctcgcggta aacaggtgcg gggactgcgc cggccggtgc gtcgtcgccg aacggaccga
    28981 tcaccgactc gacgtagcgc agccaccacg ggctggcgtc gtatccgaaa caggttggct
    29041 ccatccagat gtccgggctc gcctcgcgga ccgccgcaaa gatatcgatg atcccttcgg
    29101 ccgtgacctc gcgcgagagc tcgttgggca gatggccgtg atccgcttcc ttgcattcgt
    29161 gcctgtagcc gtcgaacttc atctgcccga gcttgaaccg gttggccagc ccggcgatcg
    29221 cctcctttgc agcagcctgg tatcgcgttc ccttcgcaag gcagcaaacg cggtagtccc
    29281 tgatggtgga actggccagc gactgcgtct catagccttg cttggcggcc caaagactgt
    29341 caaacgactg cggtgtgtag ttgttgctgg gcgaccacca caggcccaag cggcaggatt
    29401 gcgcggccaa ggcacggttc agcgtcgcga actcgccggg gaatctcgaa cgatcgatct
    29461 gccaaatgcc tgttcgagcc gaccagccca tgtcgagggt gaatgaatcc atcgccgcgc
    29521 cgaacgggcg gtaaagctga tcttcgaacg tcttgatcag gctgaggacg tctttctcgg
    29581 agaaagggac cggcgccgtc caccaactgt tgtagttgaa gtggggacct tttcctttgc
    29641 ggcgaaacga atcgacgtat cgctcgaacg attct
//
LOCUS       DAOTPW010000033        29536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1650_length_29536_cov_5.324344, whole genome shotgun sequence.
ACCESSION   DAOTPW010000033 DAOTPW010000000
VERSION     DAOTPW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 29536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29536
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1650_length_29536_cov_5.324344"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(414..1679)
                     /locus_tag="P5316_04690"
     CDS             complement(414..1679)
                     /locus_tag="P5316_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84286.1"
                     /translation="MAKMYYNLEETLARLGLTEDKLKALVREGKLREFRDAGKLNYRV
                     DEVERLSKEEGAPLSLGDSDVLPLAPPEEDTPSASRFGKTSAAPGGSGSGFELGTDAS
                     GLKIDEGTGDFKLSDSGGSGGISLSDSGGGSDSSGVLALEDSGSGSGVISLEDSGGGS
                     GPTALSDSGPAAGQKRKPSDSGSGSDLGRDIVSLDEVDKSAVEGMKKDDTVITNIGIS
                     VFDDDDLEIAADPMAKTLMTGGDEHLGLDGSGGGSGLLELTRESDDTSLGAELLEGID
                     MGDTAETALAATGPTVTEPAPEEPAEALPAAEESFAPAAVAMPMVIGTVEAASPAFTG
                     LLISGTLVMGLLMAANVAMAFGTCPGYLLALTEQFWFLLLGCLLVGGLGAGIGWFVGN
                     QASKPRIPKPPKEKKDKKAGKGKAAPAAG"
     gene            complement(1762..2301)
                     /locus_tag="P5316_04695"
     CDS             complement(1762..2301)
                     /locus_tag="P5316_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84287.1"
                     /translation="MQLGSDVTSSDVAHAIDAGADRGQPVVVVVREGGRHTVWWVIAV
                     LLAIIASALVLRLDEGRFMRSAVAQTGGAGGGLVGARGIYAFSGQLTPRTYGVFMLDV
                     DTGTIWCYELDRASNDELLLRLVAARTWTSDRYLEEFNVADPVPGAVRMMVQQQRAIR
                     QDQADLPVTSAPAKGPVLP"
     gene            complement(2528..3196)
                     /locus_tag="P5316_04700"
     CDS             complement(2528..3196)
                     /locus_tag="P5316_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84288.1"
                     /translation="MDASDQVVFVLQLVLALGPLAVYFLGLGLVNSQAHPCMVPARTD
                     FVVLTIALVPLIMGPLLAMVEFGGWWPVAVACVIVAGFVGLLLPRRNSGWVIYNTSLP
                     QCRRLLYRACRRLGFSADGNEEQVQIAEIGLTIVLTSLPVLRNVTLSLRTQPGRSANA
                     NDAVAETLMQTLESEIRKESMLPSPAGASMVVIGAAMLAVPMWFFFHNVDAIARAVRQ
                     IFFA"
     gene            complement(3271..3471)
                     /locus_tag="P5316_04705"
     CDS             complement(3271..3471)
                     /locus_tag="P5316_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84289.1"
                     /translation="MSDELLEFVMAIDEYKRLNNRPFPTWSEVFEVIRYLGYRKVAAK
                     GQHIDRPAADPLPAAPPAEATQ"
     gene            complement(3645..3920)
                     /locus_tag="P5316_04710"
     CDS             complement(3645..3920)
                     /locus_tag="P5316_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84290.1"
                     /translation="MTWMEKITASDGIAAALEEPDETEEQETVSPGELPLIVVCARDP
                     DSDDDDDFEEDDEDFDYFYDDDDEEDFDDEFDEDLDEEEIEDEDDDS"
     gene            complement(4050..5096)
                     /locus_tag="P5316_04715"
     CDS             complement(4050..5096)
                     /locus_tag="P5316_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84291.1"
                     /translation="MVRRAMMAALLVFGFAALAAAQQSLDSLPASTSAPTETAPVTTR
                     PQARVGLNEVCPVTWRGTPLREAFAELAERLGVRYILDSSIPPAALDEPVRLSASHLT
                     GQQAFRWLARIGGLSAVLVDGVFLVAAEDRLPAVWRLTGTGSLPDRPSEAARWAKVNA
                     RKADINWVDAPLAGVAEDVAALFGVDLLFHPALLADPKLLYVRENGVDLDRVREVVSR
                     QLKARTTLYDGALWVHPEGENVHWLPAIPDQGKENAPESDGLYDSPLDSWLIVDRSVT
                     TWAAFAEAVSTSAGVSCVLDDQAGTAYPVLETAGSVAEVLEGLRMLKLVAWNIAPQGP
                     SGAPTINIKLRENR"
     gene            complement(5642..8572)
                     /locus_tag="P5316_04720"
     CDS             complement(5642..8572)
                     /locus_tag="P5316_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84292.1"
                     /translation="MKNVLCLALAMTLIGLLAAPATAANLLLNPGFEEGYSTYWTRNQ
                     GSLDGTGLGEEAHSGTKSWHGAWNYAGSAQTTTYYQDVPVLPGAVCNASMWCKANAWG
                     GIYGNDTHSFRLRIRFRDAAGNNIETWDATSTPNDTWQQPSITNKTAPVGTVVCRVLF
                     SYITTQPANAWKVWNIDDLVFDAGNDGEQPIVTGITPYLIANNLSNTSATVTGVRLTG
                     VTAVKLVQGATELVGGNIVVAGDGKSLTVDFPTTGAPYGSYNVVVEKTGYAPAFLNNA
                     FRIRDPLASLLVNGGFEQGTPGSDTAAIPGWTRYNSDWGGGMANSRIDSGGWIHNPKL
                     NGVETYVIEGTYSFRTGEWYANGGEGGLVQQVPVVPGEQLVLSWWWAAGYEQPNFAIE
                     VGVLKGAYTTAWNYYGPDNLGLVRVENFPPQSYDWDDASLTFTVPAGVTTVTVYNKIW
                     NNGPNQAIALWMDDMKLLAPSCPNQHTVTGFNPSITDPVSEFDLTLTGAYLDQVSAIR
                     LGRGLDQYPGTITAQSSGSLTAHFVPPPEGMTLGLYDIITEQAGCFGQIQKQVFEVGC
                     GMYALTGVTPNAFVKPQGVVELTVSGMNVDLLTEVKLVRMLEPRNPPGDRAPYWEPET
                     TIVGTVVDATDPNAVVMSFDLHNAQAGNYRLVGTAGACTLTVNNAFSLALPTGDSIVV
                     DGGFEEASSAWVLTPDELTTDKGDPMPGASIAYTNWVGPGDPGNYGYPKISGIDPPYT
                     GDEVHLARSGNYLAGCYAFAYGSNDPVLNWNTWVSPNNGKLSQSLGLPNGPGNYALVL
                     TYWVRFWDQDWPGGTLKASIIVDGAEASSTTIGFPEYGKATQDGYDPYTQLSVDFIGS
                     AQNDITIEFYFQTVGGTGANSSATTAIVIDDVAVLGAKLCGNPFADADRDGDVDQADF
                     ALFQACYTGPNGTVADECRCFDRKDPLQALPDGDVDQTDMAAFEACASGPGIAADPAC
                     DDDN"
     gene            9604..9768
                     /locus_tag="P5316_04725"
     CDS             9604..9768
                     /locus_tag="P5316_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84293.1"
                     /translation="MIDAASANMISILAVPRVIGKQGDVAQPTCGLAKGKPGAQWGSP
                     SGTAANTPSG"
     gene            10000..10386
                     /locus_tag="P5316_04730"
     CDS             10000..10386
                     /locus_tag="P5316_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84294.1"
                     /translation="MAGVAEEDRCRLAAILCIGGLLFVLLGVFLTLQYQYDVYETTTS
                     GPGPLPDNKATARMLQAVLFWLLLLLGIFMVSTLAFLRWSRRFRKYLLARPHPPTPVD
                     DVWSMHRLPEEAAGLASPPNQGGEPG"
     gene            10509..11639
                     /locus_tag="P5316_04735"
     CDS             10509..11639
                     /locus_tag="P5316_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84295.1"
                     /translation="MSLTPIGPVDLFSRSNRWAPHAIISILIAVGALTVLALPAVVVL
                     VARQGVVTGMEILAIVGLLIGVLSVCGMLIIGIIVYRQTEALVEKLTAIDQQTAILAE
                     QSMARTQAHTYAQSTSVNPAELRTLLERIHEVLLLPEQERANRYRAMVEREFGRRLAV
                     IDHLISTNEFHQAREELTALSERFGQDRRILVAADRLEKAANAALANDVARVTQFVKD
                     VVAQGRWDEAERTVLELARKYPSATEPNALLERLRQEKKKFEQLNRQRMHDEIQQFVN
                     QKRWQEAHSAARKFLETFPTGPDSDVLRNQLPTLEANAEIQARQTLERYIKQYIQKQQ
                     YWDALALARRILNEYPLSPQANALRGQIGRLEELARRQAPQS"
     gene            complement(11743..12552)
                     /locus_tag="P5316_04740"
     CDS             complement(11743..12552)
                     /locus_tag="P5316_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRR84296.1"
                     /translation="MRKQGAFLLVIGLLSTGPWSVGCSTVAETGRKQIMLVSAEQEQR
                     LGLDAYQEVLAKEKRSSDPRMTAIVERVGRRIAAVANRPDFQWEFALLESDQVNAFCL
                     PGGKIAVYTGLLPIARNEAGLAVVVGHEVAHAVARHGGERLSQRLSAEIIQELLATGL
                     SRASPMVRTGALQAFGIGTNVGVLLPYSRTHETEADQIGLMYAARAGYDPREALALWQ
                     RMEAAGKNRPPEFLSTHPREARRIERLGKLMPQAMKAYEASPHRYGLGETW"
     gene            complement(13204..13878)
                     /locus_tag="P5316_04745"
     CDS             complement(13204..13878)
                     /locus_tag="P5316_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRR84297.1"
                     /translation="MIMTQTDSSVADAPGLLRPAAPRWRRTVGWAYRRIRGLLALSML
                     ILIVLVFTPLTGWLQDLLDVTQPPQAADYIVCLGGEDERLIWAAELFKQGYAPRVIVS
                     NIGVSALVMKSRIENMGIPPDRLMVDENSRTTADHPGSIAALEGINPAAHRFLIVTNL
                     EHSRRAAACFRKGGYAHFSVFVGRPSTKQEGPLTARNWRARARILPTLAYEYTAIMKY
                     WLQDKI"
     gene            13984..15582
                     /locus_tag="P5316_04750"
     CDS             13984..15582
                     /locus_tag="P5316_04750"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRR84298.1"
                     /translation="MAASINQSGNQPNAEQEAGRSAFDTFAGWLLVFLASLCLYAATA
                     NRGAQWQDSGEFILRITTGDLLGRLGLALSHPLHYWIGRAVIAPRLLEPAFAITLISS
                     LGAALAVAAVYGCVRTLSGSEPAAFFAAGSLAVANTFWHLATIAEVYTLSAALLAGEC
                     WCVAVYARRGSRWAVWGMCLFNGLGLANHLQSVLTLPVIAVVILHGIGSRRLKTGDVL
                     IALWLWLLGSLPYTGLVLGLMVHGADPMKTLQSALFGNHFSGAVLNARATTRMMVTDI
                     AFPLLNFPNLLIPAALYGLAGYRRAGVPRLAWLAMIVGLAIHAVFVLRYEVLDQHTFF
                     LPMYTFLAIFGGVGAAAVLQWPSTPKRSILCTAAIFLLLVTPLLNIAAITMARRAQLL
                     GRFAHRKPYRDDYIYLLVPWSAVDRSAERMSNEAVSLAGPDGLILVEDGMARSAVEYR
                     RLQAGLPGIHVRMVPAAGQQEELERLATDVRVEAQAGRAVVLVPLNRDQPRLAPPLGR
                     WERHGDLYVLDAAAGTPASAETAP"
     gene            15969..16685
                     /locus_tag="P5316_04755"
     CDS             15969..16685
                     /locus_tag="P5316_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84299.1"
                     /translation="MFETLTLLADHALLWAGPVPMAAIYIVSLILGGGMLIVSSILGG
                     HASTDVQVDGGFDFSGDVHTDLPGDMHVDPSGGAEGAFDAAGHGHDWLSLTSWFSISF
                     VVYFLAVFGLVGTSLTYLSNVQPHGVAALAVLTGLLAGQGVHHLLRFLRRTSGNSAPG
                     IAEYVNRSACVTLGINGHSKGEVSVSVRGGMRFIPAVARQPNARFEKGDRVVIVALND
                     ATAEVVSHDEYVSSKGSEPV"
     gene            16707..18095
                     /locus_tag="P5316_04760"
     CDS             16707..18095
                     /locus_tag="P5316_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRR84300.1"
                     /translation="MHTLILAAPPGADSFLGPALIIGLGLLGVAIAIALLISKLMVVG
                     NPSEMLVISGRRSAQGQGYRTLIGGRTLVIPIIEKVSRISLRNMQVGLEVSAQSGGGK
                     MIPVTVTGVANVKVSSEPDLRGNAIERFLGEPMQELQRVARETLEGGLRAVIGKMTPE
                     EIVEDRDKFVATVMREVTDDFRKLGLTIDSVNIQNVHDAEQYLESIARKASAEVRASA
                     RQYEAQKKADADIKEAEADAMARKARADRDSEARSAEAAAHQRAESARLAAEQSIAEA
                     ENALRIRRAELAREAELKEAETQKAAAAKEEARLVAAQVKPAIAARDVAKAKAEGDAA
                     LIMEEGKARAAAIETIGKAIQAHPDALKVMLAEMMPTAIREITKTVANVELGSVTVID
                     GDQGRAISGAAMGRARMISESLATLEAVMGIDLRRLAHSIAGNLGGGDGKQDTPAASA
                     HAATAPPKKQDR"
     gene            complement(18166..19959)
                     /locus_tag="P5316_04765"
     CDS             complement(18166..19959)
                     /locus_tag="P5316_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRR84301.1"
                     /translation="MVLGSAGFTHWDWIVLIVYFVGIVTFGLWMARKIRSSGGYFLGD
                     RQLPWWVMMGQAFGTGTNAENPVAQTGASCQHGFATIWYQWKNMLITPFYWLMSPWYR
                     RSNRTTIGEIIEDRYGRRLGLLYTVFAIAFFVFNQGAMLKGAGKIISVATGGQIISPN
                     GVVMAMTATFILYSFFGGLIASAYTDFVQGFLIILLSFLLIPAGLSAVGGFQGMRATI
                     PPDFFTLYSDVSGLDAFTIAMLAINGLVGITAQPHILSMNATGRTERAGRIGQMYGNL
                     VKRLCTVGWALTGLVVAAMVLQRGISLPDPEHAFGYACLELLGPGLVGLMVACVLAAN
                     MSTCSNFMVNTGALFTRNLYAHYLKPAAGDRELLMVGRLSGLALTGLGILFALTVERV
                     LDAFLFTETIAAFMGIMFLGGVLWKRANRYGAFTATIAAFGAYYALNFLMTCRVDPSD
                     ASSRTLSEAWVRMINAARSDALWGFLGNGELRLVYKWTPGPYGWATLIGFTLFVAVSL
                     LTRPEDQERTEEFFDRMRYTSDRQGQLAAECGQDLVLLDLPGWFTVERWKGFFRRYRE
                     DLVGFILGWLAVGALILLAWGILQIGRQPGL"
     assembly_gap    20112..20121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20122..20510
                     /locus_tag="P5316_04770"
     CDS             <20122..20510
                     /locus_tag="P5316_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84302.1"
                     /translation="AFSGRSTAECLIRYNLGLMNGTYGTIRSRALRREAVSPVGLAAL
                     VAALHLAVWTGRVSARWAGARPGALVIWRMPQDAIRVAAGKSAQPPSRPTQTYSVGQL
                     ALAVGMAVVTLAGWLHIMRLTGVHDE"
     gene            20643..24605
                     /locus_tag="P5316_04775"
     CDS             20643..24605
                     /locus_tag="P5316_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRR84303.1"
                     /translation="MVDILSVPGLLLRKVFGTRNERLLKRLGIVADRVLTLEEEIRQL
                     APEQLRAKTDEFKKRLADGEPVASVLPEAFAVLREASDRAQKHRHFHCQVIGGQVLYQ
                     GNVAEMRTGEGKTIVCHLAAYMKFLQGKKAHIVTVNDYLVRRDAEFAKPIFELLGATV
                     GFIQAQVDPGGHEGIRQAAYACDITYGTNSEFGFDYLRDNMKIRYEDQVQGRLDYAIV
                     DEVDSILIDEARTPLIISGPAHGDVGRYKWADSIARSLVRQQQSLNNETARRISEWGD
                     NPPEHLRANPKFEDAIKRFRVDPYMLTEDEAEAIGHRQLYVVQQDRKQVQITHEGVQR
                     AQDEAGIGSFYVGENMECPHLIENALRAHVVYERDKEYVVQDRQVIIVDEFTGRLMHG
                     RQWSDGLHQAVEAKEGVPVKEETQTMATITIQNFCKLYEHLAGMTGTAMTESEEFMKI
                     YKLEVVAIPTNRPVNRLDHNDRIYRTFKNKYEMIVEEIHEMHRRGRPNDPFLLAEVFN
                     RLKPIIQETPDLSEDRRNEQIEAIEQALQQFRDAENGDTQVTRAMMETYDRVMGNLAT
                     GRPILVGTTSVENSERLSQLLIKTYGIEHEVLNAKNHAREAEIVAKAGHRHEPTRGTE
                     NLPLGNVTIATNMAGRGTDIKLEEGVVYGKCKVPQDVRTMIPEYPAASLRLFPPGSTK
                     CCINCPEYNAATACSHCFKPKLDSRFPAMGRHICSLNAPCGLHIVGSERHEARRIDNQ
                     LRGRAGRQGDPGSSRFFLSLEDDLLKLFMPEWMLKMMERLGFTEGVSLEDKRISKGIE
                     RAQRKVEERNFSIRKHLLEWDEPMDYQRRAFYKERQQILEGRQLSDLIWRMIEETVEE
                     AVADYLAEDYMARRISDWCKNNLDVAIDAEVLEGDDAAHLQRIIREKAKDEIRDAIRT
                     SLGEYIDEEEGSAAWDVGGLLKWAQRMFPVSTTQNQLRKMTPAEIEDLLTQAADAHYD
                     QVDLSAIEAYLDPQYARRALADWARNKFGIAIKADEIAEGAEEKVKEVIYAKVREAYT
                     QREIYYPVETVLKQAFGSTGTDNVYACQAVVGWANAKYQLGWNSERFVGRPIKDIADE
                     LVQAGRDFLEGGRLEHEIDGALSRYGATGNGELYEWARKRFGKALNEKALRSGAEEPR
                     EVLLNAGRQLARWELTQLERWVLLRIYDQGWKDHLLEMDHLKHAIMQRPLGGDQTHPQ
                     SQYAIEGREQFRQMWKTIRERVTDMIFKVSHGQAGEGAEESRRTSHLEARHDSSVGAG
                     FAGASEDQQAAMRAQGEGAKPVTIRREAPKVGRNDPCPCGSGKKYKQCHGRKEPLRRS
                     "
     gene            24609..24815
                     /locus_tag="P5316_04780"
     CDS             24609..24815
                     /locus_tag="P5316_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRR84304.1"
                     /translation="MALRDNSEGNRFDRRKQREEWRKAGLPGKDEEPLPPPVEPIRAD
                     KKEVGRNDPCPCGSGKKYKKCCGK"
     gene            24815..25210
                     /locus_tag="P5316_04785"
     CDS             24815..25210
                     /locus_tag="P5316_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009572910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HRR84305.1"
                     /translation="MATLNHWFVEFWRDFEVWDKLRDPWVWFGFGAQALFFGRFLWQW
                     IVSERRGHSTIPIAFWYLSLSGGGALFIYAWHQRDLVIMLGQLLACAIYFRNLMLIYG
                     QARRRALAGLPKAKLRSEVSGENDSEAGY"
     gene            25327..25635
                     /locus_tag="P5316_04790"
     CDS             25327..25635
                     /locus_tag="P5316_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84306.1"
                     /translation="MWFAQTSRPDVWEKALPILWVGFGLILAAVVFGAAIIWWRRRSL
                     ADEPTPGEVWTLDDLRRMRAEGSLTEDEYQRLRETMIAAYKSRVAKSPEADSSPEKRV
                     "
     gene            25914..27221
                     /gene="clpX"
                     /locus_tag="P5316_04795"
     CDS             25914..27221
                     /gene="clpX"
                     /locus_tag="P5316_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRR84307.1"
                     /translation="MAGTRGPTGGTGGPGRPGRRSTSCSFCGKSQREAAPMVEGPGDV
                     YICAACVDLCQNIIKQEKRKSGPARHLASSIPPPRQIKEFLDQYVIGQEHAKRALAVA
                     VHNHYKRLAHTDGADEDDVEIDKSNVLLIGPTGSGKTLLARTLARVLNVPFAIGDATT
                     LTEAGYVGEDVENILLRLLQNADYDLEAAQRGIVYIDEIDKIGKTSQNVSITRDVSGE
                     GVQQALLKMLEGTISNIPPQGGRKHPEQQYIQMDTTHILFICGGTFVGLDEIIGRRLG
                     KSTIGFMGEMSGKSTKDSTVRDRNILRSVTPDDLVTFGMIPELVGRLPVITALDQLDE
                     GTLVRILTEPKNALVRQYQRFFEMEGCELEFTGAALKVIAERAMERDTGARALRGVME
                     DLMLDVMYRLPEEGKNGRFVVTEEVARGEVDLFTQRARRKESA"
     gene            27793..28698
                     /locus_tag="P5316_04800"
     CDS             27793..28698
                     /locus_tag="P5316_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRR84308.1"
                     /translation="MSLLVTGSIAIDDVRSPFGSAEGVYGGSAVYFSFAATLFVPVRF
                     VGVVGEDFPDDFRAFLTKRNIDLAGLEVRRGSKTFRWSGQYDQVTSDAETIAIELNVL
                     AEAGPRIPPQFADSEYVFLAATHPSLQQDLVGQLKSPRLIVADTRNLWITNERAALLK
                     TFSMVHGAILNDAEARLLTDRHNLVLAGRRIIEWGPRFVVIKKGENGVMLVTKDTVAV
                     LPAFPTTEVKDPTGAGDSFAGGMMGCLAKQRDFSEVSLKRALAYGTITASFTIEDFSL
                     RRIEKVTIDDVNRRLAEYKAMLSLE"
     gene            28787..>29536
                     /locus_tag="P5316_04805"
     CDS             28787..>29536
                     /locus_tag="P5316_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84309.1"
                     /translation="MQDSTTRRSFLQALGLGTAALGVTGIARAEEKVIQGFEKEATDL
                     NASKDWKPVSDRKVRVGIAGYGVCRFGAAFSFQDHPNVEIVAVSDLDSDKCAALAKAV
                     RCEKTYPSAEEMIKDQKIEAVFVATDAPSHARLAIMALQHGKHVCSAVPACFGSIEDA
                     DKLFEAVKTTGLKYMLFETSYFHADLYAMREIYKAGGFGKLVYAEGEYYHYMEEPIDS
                     YKGWRVGLPPQWYPTHSNAYYVGVGGGSFTEV"
BASE COUNT         5946 a         8974 c         9079 g         5527 t
ORIGIN      
        1 caaatcggac ttggcgatgt tgtattgctc ggtcaactcc gcgttggccc gcttggcctg
       61 tacccattga tcgtaatagc atccggtgtt gccggcagcc agggcgatga acgccaaccc
      121 aatcatgcat ctcttggcca ttgcagtctc ctttgccatt cgctactcgt atccgtactt
      181 cgcgtctcgg gtcggccgga aagaacgcct cggggaatcc atggggcgaa tctaacacgg
      241 cggcgctcgt tcgcttgacg cgattgaacc tgcccgaggt atatgccctt gtaccgctat
      301 cagtctctca caccgccccg ctcgcgccaa ccggacggcg gcgtgaaact cggaatggtt
      361 tgcctctcgc atgcgtcgcc gagaaacagc tgcttcggtg atggcggtct gcctcaaccg
      421 gccgcaggcg ccgccttacc cttgccggct ttcttgtcct tcttctcttt gggcggcttg
      481 ggtattcgcg gtttcgacgc ctgattgccg acaaaccatc cgataccggc gcccaagccc
      541 ccaaccagca gacacccgag cagcaggaac cagaactgct cggtcagggc caacaggtag
      601 ccggggcaag tcccgaaggc catagccaca ttggcggcca tgagcaaacc catcaccaac
      661 gttccggaga tcagcaaacc tgtgaacgcg ggcgacgccg cttcaacggt cccaattacc
      721 atcggcatag cgacggccgc aggtgcaaag gactcttcgg cggcgggcaa agcctcggcg
      781 ggctcctccg gggccggttc ggtgaccgtc ggtccggtgg cggccaaggc cgtctcggcc
      841 gtgtcaccca tatcaatgcc ctcaaggagt tccgccccca gagacgtgtc gtcgctctcc
      901 cgggtcaatt cgagcaaccc ggagccccct ccgctgccat ccagcccaag atgctcgtca
      961 ccgccggtca tcagggtctt ggccatcggg tcggcggcga tttcaaggtc atcatcatca
     1021 aacacgctga tgccgatgtt ggtgatgacc gtatcatctt tcttcatacc ctcgacggcg
     1081 ctcttgtcca cttcgtcaag actgacgatg tcccgcccca gatcgctgcc acttccgctg
     1141 tcagagggtt tgcgcttctg cccggccgca ggtccactat cagacagggc tgtaggcccc
     1201 gagccgccgc cggagtcctc caggctgatc acaccactgc cgctgccgga atcctcaagg
     1261 gcaagcacgc cggagctgtc cgagccgcct cccgaatcgg agagggagat cccgccgctg
     1321 ccgccggaat cgctgagctt gaagtcgccc gtaccttcat cgatcttgag tccggaggcg
     1381 tcggtgccca actcgaagcc ggagccgctg ccgccgggtg cggcggaagt cttgccgaac
     1441 ctggatgccg aaggggtatc ctcctcgggt ggggccaacg ggagcacatc actgtctccc
     1501 agcgaaagcg gtgcgccctc ttccttcgac agccgctcga cctcatccac acggtagttg
     1561 agcttgccgg cgtcgcgaaa ctcccgaagc ttgccctcgc ggaccagagc cttcagtttg
     1621 tcctcggtca ggccaagcct ggcgagcgtc tcttccaagt tgtagtacat ttttgccatg
     1681 atcgccgccc aactctcttc gcgcccggcc cgtcggccgg accgcaaacc tcagctactc
     1741 agttgtccgc aagccgccat gttaaggcaa aaccggtcct ttggccggtg cggaggtcac
     1801 cggcaggtcc gcttggtcct gccggatggc tcgttgctgc tggaccatca tacgaaccgc
     1861 cccgggaacc ggatccgcga cgttgaactc ttccaggtac cggtccgaag tccaggttcg
     1921 agcggccacc agccggagca gcaactcatc attcgacgcc ctgtctaatt cgtaacacca
     1981 gatggtgccc gtgtccacat ccagcatgaa cacgccgtaa gtcctggggg tcaactgccc
     2041 gctgaacgcg tagatgcccc gggcgcccac caagccaccc ccggctcctc ctgtctgggc
     2101 cacggccgaa cgcatgaatc tgccctcgtc cagtcgcagg accaaggccg acgcgatgat
     2161 cgcgagcaaa acggcgatca cccaccagac cgtatggcgg ccaccttccc gaacgaccac
     2221 cacgaccggt tggccgcgat cagcccccgc gtcgatggca tgggccacat cactgctcgt
     2281 cacatcggac ccgagttgca tggggaacct ctggttccta gcggggctga aaacgcaggc
     2341 tcttacgaag catgaactta cgcggatgcc ccattagaca ttatacacct gctggccagc
     2401 gggtcaatct ggcggaccgg caagatcgca tacccgtggc gcaaagccgc aaagaccggc
     2461 accgcaatac tcggagacgg ccaatgccaa cagggaagat gtggaatgat gtcggcgcgc
     2521 cctgttttca ggcgaagaag atctgacgga cggctcgggc aatggcatcc acattgtgga
     2581 agaagaacca catgggcacc gccagcatgg ccgccccgat gaccaccatg cttgccccgg
     2641 ccggtgacgg gagcatggat tccttccgga tctcgctctc cagcgtctgc atcagtgtct
     2701 ctgcgacggc gtcattggcg ttcgccgaac ggccgggttg cgtccggagc gaaagcgtga
     2761 cgtttcgcag taccgggagc gacgttaaga caatcgtcaa gccgatctcg gcgatctgga
     2821 cctgttcttc attaccgtcg gccgaaaaac ccaaccggcg gcacgcccgg tacaacaggc
     2881 gacgacactg cgggagactc gtgttgtaaa tgacccatcc tgagttgcgt cgaggcagca
     2941 acaaaccgac aaagcccgcc acaatgacgc atgccacggc gacgggccac caaccgccga
     3001 actccaccat cgccagcaac ggacccatga tcaatggaac aagggcaatg gtcaacacca
     3061 caaaatccgt tcgtgccggc accatgcagg ggtgagcctg actgttgacc agccccaggc
     3121 cgaggaagta gaccgccaac ggcccgagag ccagcaccaa ctgcagaaca aacaccacct
     3181 gatcagatgc gtccacgatt gcctgtcacc tctatgcgat cggtcgcggc ccccagccgg
     3241 cagcgaaaca aagatcgatc cttttgcctg ttactgcgtt gcctccgcag gtggagccgc
     3301 aggcagagga tcggctgcgg ggcgatcgat gtgttggcct ttggccgcca cttttcgata
     3361 acccaaataa cgaatcacct cgaagacttc gctccatgtg ggaaacgggc ggttgttaag
     3421 gcgcttgtac tcatcaatgg ccatgacaaa ctcaaggagt tcatcactca tctccccttc
     3481 ttcagcttgt cgtcgcagat cgctccttcg tcgccccggt ccgcgtcgtc tctccaggcc
     3541 ggcgcgacga tccaccacgg atcgccgctc gccctgccgc cgttcaatcc cggtatactc
     3601 ttgagccacg gactctcccg ataccaccgt caagcgtttc gagatcagga atcgtcatcc
     3661 tcgtcttcga tctcttcttc gtcgaggtct tcgtcaaact cgtcgtcgaa gtcctcctcg
     3721 tcgtcgtcat cgtagaagta gtcgaagtct tcgtcgtcct cctcgaagtc gtcatcatcg
     3781 tcgctgtccg gatcccgagc gcagacgaca atcagcggca gttcacccgg gctgacggtt
     3841 tcctgctctt cagtctcgtc cggctcctca agcgccgccg cgatgccgtc gctcgccgtg
     3901 attttctcca tccaagtcat gacccaagcc tcccttacac gtgaatttca ggccttctcg
     3961 ccgcgatcac gaccggcaaa gtgtatagcc gatgtctgcc tcgtcaagcg attttaccct
     4021 gtgtgatcga ccaaaacctc aaaaaatccc taacggtttt cccgcagctt tatattgatg
     4081 gttggcgcgc cggaaggccc ttgtggggcg atgttccagg ccaccaactt gagcattcgc
     4141 aggccctcaa gaacctcggc gaccgaaccg gccgtttcga ggaccggata agcggtcccg
     4201 gcttgatcat caagcacgca agagacacca gccgacgtcg agacggcctc cgcaaacgcg
     4261 gcccaagtcg tcaccgaccg atccacgatc agccagctat ccaacggaga atcatagagg
     4321 ccgtcggatt cgggggcgtt ttccttgcct tggtccggga tagccggcag ccaatgaaca
     4381 ttctccccct ccgggtgcac ccaaagagca ccgtcataaa gcgtggtccg ggccttcagt
     4441 tgccgactca caacctctct gactcggtca agatcgacgc cgttttctcg gacgtacagt
     4501 agtttgggat cggccagcaa ggctggatga aacaaaagat ccactccgaa caaggccgcc
     4561 acgtcctcgg caaccccggc aagcggagcg tctacccaat tgatatcagc cttcctggca
     4621 ttgaccttag cccacctagc tgcctccgaa gggcgatcgg gcaaagaccc ggtacccgtc
     4681 aatcgccaga cggcgggcag acggtcttcc gccgcaacga ggaagacacc atccaccagg
     4741 acagccgaaa gacctccaat ccgagccaac cagcgaaaag cctgctggcc tgtcagatgg
     4801 ctggctgaga gtcgaacggg ttcatccaac gcggccgggg gaatcgagga gtcaaggata
     4861 taccgaacgc ccagcctttc agccagttcg gcaaaagcct cacgaagagg agtgcctcgc
     4921 caagtgacgg ggcaaacctc gttgagcccg actctggctt ggggacgggt ggtgaccggg
     4981 gcagtctcag tgggcgccga ggtggatgcg ggcagactgt ccagcgactg ctgggccgct
     5041 gcaagagccg caaatccgaa aacaagcaaa gctgccatca ttgcccgtcg aaccatcgaa
     5101 gaccccttgt cagcaagaaa ctggcctgca tggatccgca ccggctcggg tattgttaag
     5161 cgtccccgtc tgccccggca agtccgcgcc gacacgtcag acaaactgcg gaaacaccgt
     5221 ttcggcttgg caagagcagt cgtggtcctt cctccccccc cagtggcata ccaaaccgcg
     5281 aagacaaagg caatagccct gcgatattat cactaaccct ttccaggaca atgacttaag
     5341 cacaagaaag cgaacccctg aggcggactg cctttttgcc ggccataaac ccaagctcac
     5401 cgcgatccgg aaacacgcgc aagccgcaca aatgtctgat cacaaggttg tgaggttcca
     5461 ttgcaccgat aggccgatgc tcgcgacggg ggcgggagag gcagacacga ctcgcagtga
     5521 tcgctgcgac aatgcagagc ccgtgtccgc tgagaaaaaa ccggcgggag tcactggggc
     5581 tcccgccggt cttacgtgca cgcagagcgt gtccgcgcga gccgatttcg tcccccgcga
     5641 ctcagttgtc gtcgtcgcag gcaggatctg cggcgatgcc cggaccactc gcacaggcct
     5701 caaacgccgc catatcggtc tggtcgacat cgccatcagg caaggcttgc agtggatcct
     5761 ttcggtcgaa gcaccgacac tcgtcggcaa ctgtgccgtt gggaccagtg tagcaggcct
     5821 ggaacagggc gaagtcggcc tggtcaacat caccgtctct gtcggcatcg gcaaaggggt
     5881 taccgcaaag ctttgccccc aacacggcga cgtcatcgat gacgatggcc gtcgtggccg
     5941 acgaattggc accggtgcca ccaaccgtct ggaaatagaa ctcgatggtg atgtcattct
     6001 gcgcggaccc aatgaagtcc acgctcaatt gcgtgtacgg gtcatagccg tcctgcgtgg
     6061 ccttgccgta ttcggggaat ccgatcgtag tggaggacgc ctctgcgcca tcaacaatga
     6121 tcgacgcctt gagggtgcca cccggccagt cttgatccca gaatcgtacc cagtaggtca
     6181 gaaccaacgc atagttaccg ggaccgttgg gtagtccgag agattgtgac agcttgccgt
     6241 tgttcggcga gacccaggtg ttccagttca gaacaggatc atttgacccg taggcaaacg
     6301 cgtagcatcc tgcgagatag ttgccgctgc gggcaaggtg gacttcgtcg ccggtatacg
     6361 ggggatcgat tccggaaatc ttcgggtagc catagttgcc ggggtcaccc gggccaaccc
     6421 agtttgtata ggcgatcgaa gcccccggca tcggatctcc cttgtccgtg gtcagttcat
     6481 cgggagtcag cacccaggcg ctggaggcct cctcgaagcc tccgtcgact acgatactgt
     6541 cccccgttgg gagggccagg gagaaggcgt tattcactgt gagagtacag gctcctgccg
     6601 tgccgacgag tctgtagtta cccgcctggg cattgtgcaa gtcgaacgac atcacgactg
     6661 cgttggggtc agttgcatca acgacggtgc cgacgatcgt cgtttcgggt tcccaataag
     6721 gcgcccggtc acctggagga ttgcgtggtt cgagcatacg gaccagtttc acctcggtga
     6781 gcaagtccac attcatcccg ctgacggtta gttcgacgac accttgcggc ttgacaaatg
     6841 cattcggagt cacaccggtc aaagcataca tgccgcaacc cacttcaaac acctgtttct
     6901 ggatctgacc aaagcacccg gcctgctcgg tgatgatgtc atagagcccc agtgtcattc
     6961 cctctggcgg aggcacgaaa tgagcagtca acgacccgct gctctgagcc gtgatggtgc
     7021 cgggatactg atccagcccg cgccccaggc gaatagcgct cacctgatcc aggtaagcac
     7081 cggtcaatgt cagatcgaac tcgctgaccg gatcggtaat cgatgggttg aagcctgtga
     7141 cggtgtgctg gttcgggcaa gacggcgcca gaagcttcat gtcatccatc cagagggcaa
     7201 tcgcctggtt gggcccgttg ttccagatct tgttgtagac cgtcacggtg gtgacgcctg
     7261 cgggtacggt gaaagtcaac gatgcatcat cccagtcgta ggattgcggc gggaaattct
     7321 cgacccgcac caagcccaga ttatccggcc cgtagtaatt ccaggccgtt gtgtaggcgc
     7381 ccttaagcac gccaacttct atggcgaagt tgggctgctc gtatccggcg gcccaccacc
     7441 aggagagcac gagttgctca ccaggaacga ccggcacctg ttggacaaga ccaccctcgc
     7501 ctccgttggc gtaccattct cccgtgcgga acgagtatgt gccctcaatg acgtaggtct
     7561 cgactccgtt aagcttgggg ttgtggatcc acccgcctga gtcaattcgc gagttggcca
     7621 taccgccacc ccaatcactg ttgtatctgg tccacccggg gatcgccgcg gtgtctgatc
     7681 ccggagtgcc ctgttcaaag ccgccgttga ccagcaacga cgccaagggg tcccggattc
     7741 gaaaagcatt gttcaagaat gccggcgcat aaccggtttt ctcgaccaca acgttatagc
     7801 tgccgtatgg tgcaccggtg gttgggaagt ccacggtgag cgacttcccg tcgccggcaa
     7861 ccacgatgtt ccccccaacc agttccgttg ctccctgaac cagcttcacc gccgtcacgc
     7921 ccgtcaaacg gacaccagtc accgtcgccg atgtgttgct gagattgttg gcgatcaaat
     7981 aaggggttat gcccgtaacg atcggctgct cgccatcgtt gccggcgtca aacacgagat
     8041 cgtcgatgtt ccagactttc caggcgtttg ccggttgcgt cgtgatatat gaaaacaaga
     8101 cgcgacagac gaccgtcccg accggagcgg ttttgttcgt gatactgggc tgctgccatg
     8161 tgtcgttggg agtcgaagtc gcatcccaag tctcaatgtt gttgcctgct gcatcgcgaa
     8221 aacggatacg caagcggaag gagtgcgtat cattcccgta gatgccgccc caggcgttcg
     8281 ccttgcacca catggatgca ttgcatacag cgcccggcaa cacggggaca tcctgatagt
     8341 acgtggtggt ctgagcagag ccggcgtagt tccacgcccc gtgccagctc ttggtcccgc
     8401 tgtgtgcttc ctccccaaga cccgtgccgt cgaggctgcc ttggtttcgg gtccagtagg
     8461 tggagtatcc ttcctcaaac ccggggttca acagcaggtt tgcggccgtc gccggagcgg
     8521 ctagaagtcc gatcaacgtc atagccaggg ccaaacaaag aacgttcttc atgtgatcct
     8581 cctccacagg ttttgcgtgg accgggcgaa gtccggtcat cacggcttga tcaataaaat
     8641 cggtcatcat cgatcagggt cgacccccca taatcgattt taggactgct gcggggcgat
     8701 tgcgcgaccc gtcgctgaaa cttcaccttc actagaaccg ggctgactga aggccggaac
     8761 cagcttctca gatcatccct ccaagagacg ccggcggact gcatcgtggc gtccgggggc
     8821 tcactcgtcg ccagcaaaac ggacggcagc aacacctgcc agagggctcg catgcttcct
     8881 acgattgggg gccgatcagg tcctgcttta cgctcccgga cggccagaat agcccaagaa
     8941 catcaaggcg aacgcgagcc tcgcagtgcc gcctttcgca agatcaccac gaccgcaatt
     9001 ttacgccttt ccactgtagc ttcttctgat ggcgtcctga tacacgccaa ccctgcggtc
     9061 gaggtagatt gtccgcgact aactgcctct caggttctac tataaggtct cgacaacaaa
     9121 acgtcaacca tgagatcggc tgtttgttgt atcgaattac ctttctccag aaagcgtttc
     9181 tgggatcctg agccacagca acagcattaa ggatgtttgc tcttggcgat ggctgtgaaa
     9241 gtggcactga acaagcggag atgtgcatca cgggagatca gaaaggccct tcggagcggt
     9301 cggctggctc cttttctcgg ccaaaccccg gcgtggaccg ccacggagcc cgcaagccgc
     9361 cttgtcgggc ggtgggagac atgagcgcag gttatggatc attccactcg ttccgattcc
     9421 ccagccgcgg tacctgcctg ctgcggcgtt gacgagtcgt ttgcgtgctt gggggaggga
     9481 tcatcgggtg gcttgggcgg gcaattcagt ctctcgcagc aacagccgca acccgatccg
     9541 cttttgtgcc gtagcaggat gccgaggaag ccgcccgccg cagccagtag caggataatc
     9601 gcgatgatcg atgcagcatc agccaacatg atctccattc tggccgtgcc gcgagtgatc
     9661 ggcaagcagg gtgatgtcgc ccagccgaca tgcgggttgg cgaaaggcaa gcccggggcg
     9721 cagtggggct caccgtcagg cacagcggca aacactccgt caggctaacc tgacgggcaa
     9781 ggacggatct ctgtaagcgc tttcagttca gcaacttgca actggatggt gagccaagta
     9841 cgagtgtgat tcacgacctc aacccgcttg gccgatttcc attgagaagt tctcgcggat
     9901 tgccggaatg gaacctgtgt tgtggctgga cttgccggcc ggcacctggt ctggcataga
     9961 attagcggac actcgaggtt tcacggacag gaacagacaa tggccggcgt agcagaggag
    10021 gatcgctgcc gtttggcggc catcctatgt atcggtggac tgcttttcgt tctcctcggc
    10081 gtttttctaa cgttgcagta ccaatacgac gtctatgaaa cgaccacctc gggccccggg
    10141 ccgcttccag acaacaaggc gacggccagg atgctgcagg ccgtattgtt ctggctgctg
    10201 ttactgctcg ggatcttcat ggtaagcacg ctggcgttcc tgcgctggag tcgaagattc
    10261 cggaagtatc ttctggcgcg tcctcatcct ccgacgccgg tggacgatgt gtggtccatg
    10321 caccggttgc cggaggaggc ggccggtttg gcatcccccc ctaaccaagg cggcgaaccg
    10381 ggctgaaagg ggagcaggcc gcagttctcg tggcccccgc ctggggaggt gccgatataa
    10441 ccgctggacg ggcctgcgcg gctcgtgcca atcctgctgc aagaggctag tacgccggag
    10501 gtgtcggcat gagtttgacc ccgatcggcc cggtcgatct tttcagtcgt tccaaccgct
    10561 gggctcctca cgcaatcatc tcgatcctga tcgcggtcgg ggctctgacc gtcctggcac
    10621 tgccggccgt ggtggtgctc gtcgcccgac agggtgtggt gacgggaatg gagatactgg
    10681 cgattgtcgg gcttctcatt ggcgttctat ccgtctgtgg catgctgatc atcgggatta
    10741 tcgtttatcg ccagacggaa gccctggtcg agaagctgac cgccatcgac cagcagacag
    10801 ccatccttgc cgagcagagc atggctcgaa cacaggccca cacctacgct cagagcacca
    10861 gtgtcaatcc ggcagagctt cggacgttgc tggaacggat tcatgaagtg ctgcttctgc
    10921 ccgagcagga acgagccaat cgctaccgcg cgatggtgga acgcgagttc gggcggcgcc
    10981 tggcggtgat tgaccatctc atcagcacca acgagtttca ccaggcccgc gaggagttga
    11041 cggcgctgtc ggagcgtttc ggccaggatc gccgcatttt ggtggccgct gaccgtctgg
    11101 aaaaagcggc aaacgcggcg ttggcgaacg acgtggctcg cgtgacgcaa ttcgtcaagg
    11161 atgtggtcgc tcaggggcga tgggacgagg ctgagcggac cgtgctggaa ctggcccgga
    11221 agtatccgtc ggctaccgag ccgaacgccc ttctggaaag actgcgtcag gagaagaaga
    11281 agttcgagca gcttaatcgg cagcgaatgc acgacgagat tcagcagttc gtgaatcaga
    11341 agcgctggca ggaggcccac tccgcagccc ggaagtttct cgagaccttc cccaccggcc
    11401 cggattccga cgtactgaga aaccagctac caacgctcga agcgaacgcc gagatccagg
    11461 cccgccagac gcttgagcgc tacatcaagc agtacattca gaagcagcag tactgggatg
    11521 ccctggctct ggcgcggcgc atcctgaacg agtacccgct cagtccgcag gctaatgccc
    11581 tgcgcggaca aataggccgg ttggaggagc ttgcgcggcg tcaggcaccg caatcgtgat
    11641 tctctcgcgc ggttgcggcc cgcgggtacg cccgtcatcg atcatgggcc ggcttgcttg
    11701 ggaacccttg ctaccgcccc ggtcctgcca cgggctggtt catcaccacg tttcacccag
    11761 cccatagcga tgcggagagg cctcataggc tttcatggcc tggggcatga gttttccgag
    11821 ccgctcgatt cgccgtgcct cacgggggtg ggtggacagg aactcgggtg gacggttctt
    11881 gccggccgcc tccatccgct gccacagggc caaggcctct cgcgggtcgt agccggccct
    11941 ggccgcatac atcaggccga tctgatcggc ctctgtctca tgcgtccggc tgtacggcag
    12001 gagcacgccc acattggttc cgataccgaa ggcctgcaat gcccccgtcc gcaccatcgg
    12061 tgaagccctg ctcaggccgg tagccagcag ctcctgaata atctccgcgc tcaggcgctg
    12121 gctgaggcgt tccccgccgt gtcgtgcgac ggcgtgtgcc acctcatgac cgacgaccac
    12181 ggccagtcca gcctcattcc tggcgatggg cagcagcccg gtgtaaacgg caatcttgcc
    12241 gccgggcagg caaaaggcgt tgacctggtc ggattcgagc agggcaaact cccattgaaa
    12301 gtcaggacga ttggcaacag cggcgatgcg cctgccgacg cgctcgacga tggccgtcat
    12361 ccgcggatcg ctgctgcgtt tctctttggc cagtacctcc tggtaggcgt cgaggccgag
    12421 ccgttgttcc tgttcggccg acacgagcat gatttgtttt cgccctgtct cggcgacggt
    12481 ggagcacccc accgaccatg ggccggtgga gagcagaccg atgacgagca gaaatgcgcc
    12541 ttgttttctc atggcgttgc acctccctct gtcaacagtt gacacaagcg gtgttccatg
    12601 ccggcttatc ataattggac acatcaggct gacaaaagac aggccggggt gactgcgagg
    12661 tcagcgtctg tggcagcatc ggctggttgc gtaaagggag acgggctcac cggggcatat
    12721 ccgcacggac aacgttcagt gtcgaacgat cgccgggagt ggtcgcgggt tttggacaag
    12781 agcacgccgg ccgtgccgtg gacggcaaga cctcgattga tccgcagtcc ggaaaaacaa
    12841 acgggcggcc gcaatgaccg cccacttgcc gaaaaacggt tacctcactt gcagcagcat
    12901 ttcttgctgc tcttcttggc ggtcttcttg ccgccggctt tcttggcttt cttcttagcc
    12961 atggcgcacc tccttgcacg atcaacgaag gccccgcgtg acgcgaacgg gaccgataca
    13021 caacctagca tcactcggca gcgccgtcaa cctctttccg gtcgaaatcc gactgtgatc
    13081 cggggagaga tagtccatcg tcgccggacg gcaaggcggg ctgcgcagct tcacgattcg
    13141 gcgtggttcg aaagggcgtt aacgagtaac ccaaagcatg aacaccgccg gaaacggtca
    13201 gccctagatc ttgtcctgga gccaatactt catgatcgcg gtgtactcat acgccaaggt
    13261 cggaaggatt ctcgccctcg cacgccagtt gcgggcggtc agcggtcctt cctgtttggt
    13321 ggaaggtcgg cccacgaaca cgctgaaatg tgcatatccg ccttttcgaa aacaggccgc
    13381 agcccggcga ctatgctcca gattggtgac gatgaggaac cgatgcgctg ccgggttgat
    13441 gccctcaaga gcggcaatgg accctgggtg gtcagcagta gtgcgagagt tctcatcgac
    13501 cataaggcgg tcggggggaa tgcccatgtt ttcgatccgg ctcttcatga ccagcgcgga
    13561 gacaccgata ttggagacga tcacccttgg ggcgtatccc tgcttgaata gctccgccgc
    13621 ccagatcagc cgctcgtctt caccgccaag gcacacgatg taatctgccg cctggggcgg
    13681 ttgagtcaca tccagcagat cctgcaacca tcccgtcagc ggcgtgaaga ccagcacgat
    13741 cagaatcagc atgctcagcg ccagcaaccc gcggattcgg cggtaagccc atccgacggt
    13801 tcgacgccat cgcggcgccg cgggacgaag caaccccgga gcatctgcaa cgctactgtc
    13861 cgtctgagtc ataatcatcg catggtctcc tcggccttac gaccccacat ggttgccgca
    13921 ctcacagccg gctgcaagct gcacacgctt ccccaattcc atggccgcac gtaagatgcc
    13981 atcatggccg ccagcatcaa ccagtccggc aaccagccca acgccgagca agaggcaggt
    14041 cgatccgctt tcgacacctt cgcgggctgg ctgctggtgt tcctcgcaag cttgtgcctt
    14101 tacgccgcga cggccaatcg gggtgctcag tggcaagact ccggggaatt catactccgc
    14161 atcaccacgg gcgacctgct gggacgcctg ggactggccc tatcccatcc gctgcattat
    14221 tggatcggcc gtgcggtcat cgctcctcga ctgcttgaac ctgccttcgc gatcaccctg
    14281 atcagcagcc tcggggccgc cctggccgtt gcggcggtgt acggctgtgt gcgcacgttg
    14341 tcaggcagtg aaccggcagc gttttttgcc gcgggctcgc ttgcagtggc caacaccttc
    14401 tggcatctgg ccactattgc tgaggtctac actctcagtg ccgcgctgct ggcgggagag
    14461 tgctggtgcg tggcggtcta cgccaggcgg ggcagccggt gggccgtctg gggtatgtgt
    14521 ctgtttaacg gccttggcct ggcgaaccat cttcagtcgg tgttgacctt gccggtcatc
    14581 gcggtggtga ttcttcatgg catagggtcg cggcgattga aaacgggcga cgttttaatc
    14641 gctttatggc tctggctgct gggatcattg ccatacacgg ggttggtgct tgggttgatg
    14701 gtccacggcg ccgacccgat gaagacgctt cagtcggcgc tcttcggcaa tcacttttcg
    14761 ggggcggtcc tcaacgcacg cgccaccacc cggatgatgg tgaccgacat cgctttccct
    14821 ttgttgaatt tcccgaactt gctgatcccg gccgcactgt acggccttgc cggatatcga
    14881 cgagccggcg tgccgaggct tgcctggctc gcgatgatcg tcggactggc catccacgcc
    14941 gtttttgtct tgcggtacga ggttcttgat caacatacct tttttctgcc gatgtacact
    15001 tttctggcga tcttcggcgg tgtgggcgcg gcggccgttc tgcaatggcc gtccacgccg
    15061 aagcgctcga tcctgtgcac ggcggcgatt tttctgctgc tcgtgacgcc tttgctcaac
    15121 attgcggcca tcaccatggc aagacgtgct caactgctgg gtcggttcgc gcaccgcaag
    15181 ccctatcgcg acgactatat ctacctgctg gtaccctgga gcgccgttga ccgttcagcc
    15241 gagcgtatga gcaatgaggc ggtgagcctg gccggaccgg acgggttgat tctcgtcgaa
    15301 gacggcatgg cccgaagcgc tgtcgagtac cgccggctcc aagccggttt gcctggcatc
    15361 catgttcgaa tggtaccggc tgcggggcag caagaagagt tggagagact ggcaacggac
    15421 gtgcgtgttg aggctcaggc cggtcgagcc gtcgtgctcg ttcctctcaa ccgcgatcag
    15481 ccgcggcttg caccgccgct gggccggtgg gaacgacatg gcgaccttta cgtacttgat
    15541 gccgcggccg gcaccccggc ctctgcggaa acggctccgt gacggaccca ggcaagcttc
    15601 ggctcagcac catatttcag caccgtattg tcagagacca cccgccctgt aataaaccgc
    15661 ttgcaggagt gtccaacagg ctgaacaagc agcgcccaag atgaatcatc tctgcgccgt
    15721 gggtatcgcc atgacggtcg gccggcagaa ggtgtcggta gtatgtgttc agataattcc
    15781 ttttatggaa agcatttacg attccagtgc ggcgcatggt ggaaggggct ttgccggcgg
    15841 cggttcggaa aaacggccgg gtttctaatg ggcgcagacc gccgtagcgg atagattgcg
    15901 cttatccgag aaaagggttt ctcagcaatt caattcggtg ggccactgag gagaagaaag
    15961 gacgaaccat gttcgaaacc ctgacccttc tggcagacca tgcactgttg tgggccggtc
    16021 ctgtgcccat ggccgccatc tatatcgtgt cgctcatcct gggtggcgga atgctgatcg
    16081 tctcctcgat tctcggcggg catgcctcca cggatgtcca ggtcgacggc ggctttgatt
    16141 tttccggaga cgtacatacc gatctgcctg gcgacatgca cgtcgacccg agcggcggtg
    16201 ccgaaggggc gttcgacgcc gccggtcacg gacacgactg gctgtcactt acctcgtggt
    16261 tttcgatcag ttttgtggtc tactttctgg cggtgttcgg ccttgtgggc acgtcgttga
    16321 cctatttgtc gaacgtgcag cctcatggcg tggccgctct cgccgtgttg accgggctcc
    16381 tggccggaca aggcgttcac cacctgttgc gatttctgcg tcgaaccagc ggcaacagtg
    16441 caccaggcat cgccgagtat gtgaatcggt ccgcctgcgt gacgctgggc atcaacggcc
    16501 atagcaaggg tgaagtctcc gtgtccgtga gaggcggcat gcgattcatc cccgcggtcg
    16561 ccaggcagcc caacgcccgt ttcgagaagg gagatcgggt ggtcattgtt gcgttgaacg
    16621 atgcgacggc cgaggtggtc tcgcacgacg aatacgtttc gtccaagggc agcgagcccg
    16681 tctgataccg aaggaggaca tgcaacatgc acacactcat tcttgccgca ccgcccggag
    16741 cggacagttt tctggggccg gcactgatca tcggcctcgg cctgctgggc gtggcgatcg
    16801 ccatagcgtt gctgatcagc aaactcatgg tggtcgggaa ccccagtgaa atgctggtca
    16861 tcagcggcag gcgatcggcc caggggcagg ggtaccggac gctcatcggc ggtcgcacgc
    16921 tggtcattcc gatcatcgag aaggtttcgc ggatcagcct tcgcaacatg caggtcggcc
    16981 tggaagtcag cgcccagtcg ggaggcggga agatgattcc ggtaaccgtc accggtgtgg
    17041 cgaacgtgaa agtatcttcg gagcccgacc tgcgcggcaa tgcgatcgag cgatttctcg
    17101 gcgagccgat gcaggagctg cagcgcgtgg cccgcgaaac gctggaaggc ggcctgcggg
    17161 cggttatcgg caagatgacg cctgaggaga ttgtcgaaga tcgcgacaag ttcgtggcca
    17221 cggtcatgcg cgaggtcacc gacgattttc gcaagctggg gctgacgatt gacagcgtga
    17281 atatccagaa cgtccacgat gccgagcagt acctggaatc aatcgctcgc aaggcttccg
    17341 cagaagtccg ggccagtgcc cgtcagtacg aggcccagaa gaaagccgac gccgatatca
    17401 aggaggctga ggccgacgct atggcgagga aggcccgcgc cgaccgcgac tcggaagccc
    17461 gatcggcgga ggctgccgct catcagaggg ctgaatccgc ccgattggct gccgagcagt
    17521 ccatcgccga ggccgagaac gctcttcgca ttcgccgcgc cgagctggcc cgcgaggccg
    17581 aattgaaaga ggccgaaacg cagaaggccg cggctgccaa ggaagaggca cgcctggtgg
    17641 ccgctcaggt caagccggcc atcgctgctc gcgacgtggc caaggccaag gccgaaggcg
    17701 acgcggcgct gatcatggaa gagggcaaag cccgtgccgc cgcgatcgaa accatcggca
    17761 aggcgatcca agcgcacccc gacgctctca aggtcatgct cgccgagatg atgccgaccg
    17821 cgatccgcga gatcaccaag acggtcgcca acgtcgaact cggcagcgtg acggtgatcg
    17881 acggcgatca aggccgggcc atcagcggcg ccgccatggg gcgggcgcgg atgatctccg
    17941 aatcgctggc cacgctggag gcggtcatgg ggatcgacct gcgcagactg gcccacagca
    18001 tcgccgggaa cctcggcggc ggagacggca agcaggatac gcccgctgct tccgcccatg
    18061 cggccaccgc cccgcccaag aaacaggacc gctgagcgac gcacgagatt caccggcttc
    18121 gtgacggcgt gtgatgggtg gaccgccaat ccgtgaccac atgggtcata ggccaggttg
    18181 cctgccgatc tgtagaatcc cccacgccag caggatgagg gcgccgacgg cgagccagcc
    18241 gaggatgaac ccgacaaggt cctcgcggta ccgccggaag aagcccttcc accgctcaac
    18301 ggtgaaccag ccgggcaggt cgaggagcac gagatcctgg ccgcattcag ccgccagttg
    18361 accctgtcgg tccgaggtgt atcgcattct atcgaagaac tcttccgtcc gctcctggtc
    18421 ctcgggtcgg gtcagcaggc tgacagcgac aaacaaggtg aacccgatca gcgttgccca
    18481 gccgtagggt ccgggcgtcc atttgtacac cagccgaagc tcgccgtttc cgaggaagcc
    18541 ccacaaagca tcggaccggg cggcgttgat cattctcacc cacgcttcgc tgagggtcct
    18601 gctggacgcg tcggacgggt cgacacggca ggtcatcaga aaattcagtg cgtagtacgc
    18661 gccgaacgcg gcgattgttg cggtgaacgc gccatagcga ttcgcccgtt tccacaggac
    18721 gccgccgagg aacatgatgc ccatgaaggc ggcaatggtc tcggtaaaga gaaacgcgtc
    18781 cagtacccgc tccaccgtca gggcgaacag aattcccagc ccggtcagcg ccagcccgga
    18841 gagtcgcccc accatgagca gttctctgtc gccggccgcc ggcttgaggt agtgagcgta
    18901 gaggttgcgg gtgaacaggg ctcctgtgtt gaccatgaag ttcgagcagg tggacatgtt
    18961 ggccgccagg acgcaggcaa ccatcagccc caccaatccg gggccaagca gttcgagaca
    19021 ggcgtagccg aaggcatgtt ccggatcggg cagcgaaatg ccccgttgaa gcaccatcgc
    19081 cgcgaccacc aggccggtca gcgcccaacc gacggtgcag aggcgtttga ccaggttgcc
    19141 gtacatctgg ccgatacggc cggctcgctc cgtcctgcct gtagcgttca tgctcaggat
    19201 gtggggctgg gccgtgatgc ccaccagccc gttgatggcc agcatggcga tcgtgaacgc
    19261 gtcaaggccg ctgacatcgc tgtaaagtgt gaagaaatcg ggcggaatcg ttgcccgcat
    19321 tccctggaaa ccgccgacgg ccgacagccc cgcaggaatg agcaggaacg acagcaggat
    19381 gatgaggaag ccctggacga aatcggtgta agctgacgcg atcagcccgc cgaagaaact
    19441 gtaaagaatg aaggttgccg tcatcgccat gaccacaccg ttgggcgaga tgatctgccc
    19501 gcctgtggcc acggagatga tcttgcccgc ccctttgagc atggccccct ggttgaagac
    19561 gaagaacgcg atggcaaaga cggtgtagag gagccccagc ctgcggccgt agcggtcttc
    19621 gataatctca ccgatggtcg tgcggttgct gcggcggtac cacgggctca tcagccagta
    19681 gaacggcgtg atgagcatgt tcttccactg gtaccagatg gtggcaaagc catgctggca
    19741 ggaggccccg gtctgggcga cggggttctc ggcgttcgtt ccggtgccga acgcctggcc
    19801 catcatgacc caccagggca gttggcggtc gccgaggaag taaccgccgc tactgcgaat
    19861 cttgcgggcc atccagagac cgaacgtcac gatgcccaca aagtagacga tcagaacgat
    19921 ccaatcccag tgggtgaacc cggcactgcc caagaccatg ctgctgcccg cctttccgca
    19981 cgagtgtttg cctgcttgag ccgctcgcgg ccggagtccc caacgggcag cctataaccg
    20041 aagtctggca ttgcgtcaga ggccgcagcc gggcgctcga tcgacgcgga cagaataact
    20101 gatcgggttg cnnnnnnnnn nccgcgtttt ccggccgctc gaccgcggag tgcctcatcc
    20161 ggtataatct tgggctcatg aatggaactt acggcacaat ccgaagtcgc gcactacggc
    20221 gggaggccgt ctcgccggtg gggctggcgg ctttggttgc ggcgctgcac ctggcggtat
    20281 ggaccggtcg cgtttctgct cgatgggcag gtgctcgacc gggcgccttg gtgatttggc
    20341 ggatgccgca ggacgccatt cgggttgcgg ccggcaaatc cgcccagccg ccatcacgtc
    20401 cgacccagac ctactcggtc ggacagctcg ccttggccgt cggcatggcc gttgtgactt
    20461 tggcgggctg gctccacatc atgcggttga ccggcgtaca cgacgaatga caccgcctgc
    20521 gacctttgcc gcaaacccac gcgaaccgac gaaacccctc aatcgtgaaa cggattctgg
    20581 acgacgcaaa tgacgttcaa ccccggcgta gatgacaggt cattgaagac aacggtgatg
    20641 tcatggtgga cattctgagt gttcccggct tgctgctgcg aaaggttttc ggaacgcgca
    20701 atgaacgcct cctcaaacgg ctgggcattg tggccgaccg tgttctgact ctggaagagg
    20761 aaatccgtca actcgcgccc gagcagttgc gggcgaagac cgacgagttc aagaagcgat
    20821 tggcggacgg cgaaccggtg gcgtcggtcc tgccggaagc cttcgccgtg cttcgcgagg
    20881 cgtccgaccg ggcccagaag catcggcact tccactgcca ggttatcggc gggcaggttc
    20941 tgtaccaggg caacgtggcc gagatgcgga cgggcgaggg caagacgatc gtctgccatc
    21001 tggccgcata catgaagttc cttcaaggga agaaagccca cattgtcacg gtcaacgact
    21061 acctggttcg ccgcgatgcc gagttcgcca agccgatatt cgagttgctc ggcgcgaccg
    21121 tgggcttcat ccaggcgcag gttgatccgg gcgggcatga gggcatccgc caagcggcct
    21181 atgcttgcga catcacctat ggtaccaaca gcgagttcgg cttcgattac ctgcgcgaca
    21241 acatgaagat ccgatacgag gatcaggtgc agggccgcct tgactatgcc attgtcgacg
    21301 aggtggactc cattctcatc gacgaggccc gcacgccgct gatcatcagc gggcccgccc
    21361 acggcgacgt cggccggtac aagtgggcag acagcatcgc cagatcgctg gtacgtcagc
    21421 agcaaagtct caacaacgag accgcccgcc gtatcagtga gtggggcgac aatccgcccg
    21481 aacacttaag ggccaacccg aaattcgagg atgcgatcaa gcgattccgc gtcgacccgt
    21541 acatgctgac ggaggacgag gccgaggcga tcggccaccg tcagttgtac gtggtccagc
    21601 aggaccgcaa gcaggttcag atcacccatg aaggtgtcca gcgggcccag gacgaggccg
    21661 gcatcggcag cttctacgtc ggggaaaaca tggagtgtcc gcacctgatc gaaaacgcgc
    21721 tgcgggccca tgtcgtctac gagcgcgaca aggaatacgt ggtccaggat cggcaggtga
    21781 tcatcgtcga cgagttcacc ggccggctta tgcacggacg gcaatggtcc gacggcctgc
    21841 accaggcggt cgaggctaag gagggcgtgc cggtcaagga agaaacgcag accatggcca
    21901 cgatcaccat ccagaacttc tgcaaactct acgagcatct cgcgggcatg accggcacgg
    21961 ccatgaccga gtccgaagag ttcatgaaga tctacaagct ggaggtggtg gccattccga
    22021 cgaaccggcc cgttaaccgg ctggatcata atgaccggat ctaccgcacg ttcaaaaaca
    22081 agtacgaaat gatcgtggag gagatccacg agatgcaccg ccgcggccgt ccgaacgacc
    22141 ccttcctgct tgcggaagtg ttcaaccggc tcaagccgat tatccaagaa acccccgacc
    22201 tttccgaaga ccggcgaaac gagcagatcg aggccatcga gcaagccctg cagcagtttc
    22261 gcgacgctga gaacggcgac acccaagtca cgcgggccat gatggagact tacgatcgcg
    22321 taatgggcaa tctggcaacg gggcgcccca tcctggtcgg caccaccagc gtcgagaact
    22381 ccgagaggct gtcacaactg ttgatcaaga cctacgggat cgaacacgag gttctgaacg
    22441 ccaagaacca cgctcgcgaa gcggagatcg tggccaaggc cgggcatcgg cacgagccga
    22501 cccgcgggac ggagaatctg ccgctgggca atgtcaccat cgccacgaac atggccgggc
    22561 gaggaacgga catcaagctt gaagagggcg tggtttacgg caagtgcaag gtaccgcagg
    22621 atgtgcgcac gatgataccg gagtaccctg cggcctcgct gcgtctgttt cctccgggct
    22681 cgaccaagtg ctgcatcaac tgccccgaat acaacgccgc gacggcctgc agccattgct
    22741 tcaagccgaa actcgattct cgattcccgg cgatgggacg acacatctgc tctctgaacg
    22801 ccccctgcgg tttgcatatc gtcggcagcg agcggcatga ggcccggcgg atcgacaatc
    22861 agcttcgcgg ccgggccgga cgccagggtg acccggggtc aagccggttc ttcctttcgc
    22921 tcgaagacga tctgctcaag ctgttcatgc ccgagtggat gctcaagatg atggagagac
    22981 tggggttcac cgagggcgtg agcctggagg acaagcgcat ctcgaagggc atcgagcggg
    23041 cccagagaaa ggtcgaggag cgcaacttct cgattcggaa gcatctgctc gagtgggacg
    23101 agccgatgga ttaccagcgt cgcgcgttct acaaggagcg tcagcagatt ctggagggcc
    23161 gacagctcag cgacctgatc tggcgcatga ttgaggaaac cgtcgaggaa gcggtggccg
    23221 actacctggc cgaggactat atggcccgcc ggatcagcga ttggtgcaag aacaatctcg
    23281 atgtagccat tgatgccgag gttcttgagg gcgacgatgc ggcacacctg cagcggatca
    23341 ttcgcgaaaa agccaaggac gagatccgcg acgcgatccg cacgtcactc ggcgagtaca
    23401 tcgacgaaga ggagggttcc gctgcctggg acgttggtgg tctgctgaag tgggctcagc
    23461 ggatgtttcc ggtgtcgacg acgcagaacc agcttcggaa gatgacgccg gcagagattg
    23521 aggacctgct cacgcaggcc gccgatgctc actatgacca ggtggatctt tccgccatcg
    23581 aggcgtacct ggacccgcaa tatgcccgtc gtgccctcgc agattgggcg cgcaacaagt
    23641 tcgggattgc gatcaaggcc gacgagatcg ccgagggcgc cgaggagaag gtcaaggagg
    23701 tgatttacgc gaaggttcgc gaggcctaca cccagcggga aatatactat ccggtcgaaa
    23761 ccgtcctgaa acaggcgttt ggatcgaccg ggaccgacaa cgtctacgcc tgtcaggccg
    23821 tcgtcggctg ggcaaacgcg aaataccaac tcgggtggaa cagtgagcga ttcgtcggtc
    23881 gtcccatcaa ggacatcgct gatgaactcg tccaggccgg gcgcgatttt ctcgaaggcg
    23941 gccggctcga acatgagatt gacggggccc tctcgcggta cggcgccacg ggcaacggtg
    24001 aactgtacga gtgggctcgg aagcgattcg gtaaggccct gaacgaaaag gcgctgagat
    24061 cgggcgccga ggagccccgc gaggtgctgc tcaatgccgg tcgccaattg gctcgctggg
    24121 agctgaccca gctcgagcga tgggtgctgt tgcggatcta cgatcagggc tggaaagacc
    24181 acctgctgga gatggatcat ctcaagcacg cgatcatgca gcgccccctg ggcggcgatc
    24241 agacccaccc gcaaagccag tatgccatcg aaggccgcga gcagttccgg cagatgtgga
    24301 aaaccattcg ggagcgggtg acagacatga tcttcaaggt ctcgcatggt caggcgggcg
    24361 agggggcgga ggagtcccga cgcacctccc atttggaggc ccgtcacgac tcatccgtgg
    24421 gagccggctt tgccggggct tcagaagacc agcaggcggc catgcgggcc caaggcgagg
    24481 gggccaagcc cgtgaccatc cgacgcgagg ctcccaaagt cggccgcaac gatccgtgcc
    24541 cttgcggttc aggcaagaaa tacaaacaat gtcacggaag gaaagagccg ttgaggagga
    24601 gctgacgcat ggcgctgaga gacaacagcg aaggaaaccg ctttgaccgc cgcaagcaga
    24661 gggaggaatg gcgcaaggcg ggccttcccg gcaaggacga agaaccgctg ccgccgccag
    24721 tcgagcccat tcgtgccgat aagaaagagg tcggtcgcaa cgacccttgc ccctgcggat
    24781 cggggaagaa gtacaagaaa tgctgtggta agtgatggcg acactcaatc attggttcgt
    24841 ggagttctgg cgggatttcg aggtctggga caaactgcgg gatccctggg tctggttcgg
    24901 gtttggagct caggcgttgt tcttcgggcg gtttctctgg cagtggatcg tcagtgagcg
    24961 ccgcggacac agcaccatac ccattgcctt ctggtatctg tcgctctcgg ggggaggggc
    25021 tctgttcatt tatgcctggc atcaacgcga cctggtcatc atgctcggcc agttgctggc
    25081 ctgcgcgatc tactttcgca acctcatgct gatttacggg caggcgaggc gccgggcctt
    25141 ggcgggtctg cccaaagcca agcttcgttc ggaggttagc ggcgagaatg attcggaggc
    25201 cgggtactga gccgcgggcc tgtgtcccgg gagcgacgag agatcgcaag tgcctgttaa
    25261 caagatggtt atttctattg gaccctttcc tttcgcgtcc gtataatgaa taagggagac
    25321 ggctccttgt ggtttgccca gacaagccgg ccggatgtgt gggagaaggc tctgccgatc
    25381 ctgtgggtcg gttttgggct cattcttgcg gcagtcgtct ttggtgcagc gatcatttgg
    25441 tggcggcgac ggtcgcttgc tgacgagccg acgccgggcg aggtctggac cttggatgac
    25501 cttcgccgga tgagggcgga gggcagtctg accgaggacg aataccagcg cctgcgcgag
    25561 acgatgatcg ccgcctacaa gagcagggtt gccaagagcc cggaggccga ttccagccct
    25621 gagaagcgag tttgagcgcg gttcgggcgg gcaggatttt gatctccaga aagcccgtat
    25681 cgcgtgtcga tagaaccgga cacagcctgt tatccgggtg tactcccggc gggtgtccag
    25741 ccgctgaagg tgagcgaggc gagaggtcag gcgcaggaag atcgatcggg ggtccattcc
    25801 cgtgagccgt gcgggacggg cttgcagccc gttgtgatac gggcaagacg gcaaccccac
    25861 atcgggtggg tctgacgatt gatcagacga ttgatcattg gtgagaggtt ttcatggcag
    25921 gtacacgcgg gccgacagga gggacgggcg gaccggggcg tcccgggcgt cgcagcacgt
    25981 catgcagttt ctgcggcaag agccagcgtg aggcggcacc catggtcgaa ggtcccggcg
    26041 acgtgtacat ctgcgccgct tgcgtggatc tttgccagaa catcatcaag caggagaaac
    26101 gcaagagcgg ccccgcgagg cacttggcca gctccatccc gccaccgcgg cagatcaaag
    26161 agttcctgga ccaatacgtc atcgggcagg agcacgccaa gcgggctctg gccgtggccg
    26221 tccataacca ctacaagcga ctggcccaca ccgacggggc ggacgaagac gacgtcgaaa
    26281 tcgacaagtc gaacgtcctt ttgatcgggc ccaccggatc gggcaagacg ctgctggctc
    26341 gcacgctcgc gcgcgtcctg aacgttccgt tcgccatcgg cgatgcgacc actttgaccg
    26401 aggcgggata cgtcggcgag gacgtggaga acatcctgct ccgtttgctc cagaacgcag
    26461 attatgatct tgaggccgcc cagcgaggga tcgtctacat cgacgagatc gacaagatcg
    26521 gcaagacgag tcagaacgtc agcatcaccc gcgacgtcag cggcgaaggc gttcaacagg
    26581 ctctgctcaa aatgctcgag ggcaccatct cgaacatacc gccgcaaggc ggccggaagc
    26641 accccgaaca gcagtacatc cagatggaca cgacgcacat tctgttcatc tgcggcggca
    26701 cgttcgtcgg cctcgacgag attatcggaa ggcggttggg caaatcgacc atcggtttca
    26761 tgggcgagat gagcggtaag tcgaccaagg acagcacggt tcgcgatcgg aacattctca
    26821 gaagcgtgac gcccgacgac ctggtgactt tcggtatgat tcccgaactg gtgggccggc
    26881 tgccggttat caccgctctg gaccagttgg acgagggaac cctcgtgcgg atcctgaccg
    26941 agcccaagaa cgccctggtt cgccagtatc agcgtttctt tgagatggaa ggatgcgagc
    27001 tggagtttac cggcgcggca ctcaaggtca tcgccgagcg ggccatggag cgagacaccg
    27061 gcgcccgagc tctgcgcggc gtgatggaag acctgatgct ggacgtgatg tatcgtctgc
    27121 ccgaggaagg caagaacggc cgattcgtcg ttaccgagga ggttgcacgc ggagaagtcg
    27181 acctcttcac ccaaagggcc cggcgcaagg aatcggccta gattctctca tgacgctctc
    27241 gatatcgacc catgcctcga ggtccggtgc ctcggggcat ttttgttgga accgcccccg
    27301 tctgttattc ggcgcgctgg caagcattcc cgactcgtta tagactgccc ttgctgaggc
    27361 ggtcggcgcg gtaaccgagg aggcccatta catcgccgtg agaatcaagg ataagagatg
    27421 aaggaggaag gatgtggcaa gtgaggcttt gcccgccgcc ggagataggg tgggacgacc
    27481 gggaccttcc gcgtggagga tttccattgc cattcttgcc gaacgcggga taccttccgc
    27541 gagaggatgc ggcccggaaa ggattccggg ccgagcggta gcggattccg ggccaagcgg
    27601 tagcggattc cgggccgagc ggtagcggat tccgggccga gcggtagcgg attccgggcc
    27661 aagcggtagc ggattccggg ccgagcggac accgggactt tccatctttc atcctttcgc
    27721 cttcatcctt ttgtggatga caggagcctc acggcgatgg agcgcgcctt ccgggcgcat
    27781 ggagactcat cgatgtcact actggtgacc ggcagtatcg caattgacga tgtgagaagc
    27841 ccctttggca gcgccgaggg cgtctatgga ggctcggccg tgtatttctc attcgcggcc
    27901 acgctctttg tcccggtgcg gttcgtaggc gtcgtcggcg aggatttccc ggatgatttc
    27961 cgcgcgttcc tgacgaagcg gaacatcgat ctggcagggc tcgaagtccg caggggcagc
    28021 aagaccttcc gctggtcggg gcagtatgac caggtgacca gtgatgccga gaccatcgcc
    28081 attgaactga acgtgctggc ggaggccggc ccgaggatcc ctccgcagtt cgccgacagt
    28141 gagtacgttt tcctggccgc gacgcacccc tcgttgcagc aggatcttgt cggccagctc
    28201 aagtctccaa gactgatcgt agcggacacg cgcaacctct ggatcaccaa tgaacgagcc
    28261 gccctgctca agaccttcag catggttcac ggggccatcc tcaacgatgc cgaggctcgt
    28321 ttgctgaccg acaggcacaa cctggtgctc gccggccgac ggatcatcga atggggaccg
    28381 cggttcgtgg tgatcaagaa gggtgaaaac ggcgtgatgc tggtcaccaa agacacggtg
    28441 gcggtgctgc cggctttccc gacgaccgaa gtcaaggatc cgaccggggc gggtgacagc
    28501 ttcgccggcg ggatgatggg ctgcctggct aaacaacgcg acttcagcga ggtctcgctc
    28561 aaacgggccc tcgcttacgg taccatcacc gcgtctttta ccattgagga tttcagtctg
    28621 cgacggatcg agaaggtgac catcgacgac gtcaatcgac ggctggccga gtacaaggcc
    28681 atgctatcgc tggaatgagt tgaaccggca aggaacaaaa tcgcgagcga tgggttcgca
    28741 aggcggtctg cgagaaccgc cgcgtagaga tgaaggagga ctcacgatgc aggattccac
    28801 cacaagacgt tcgtttctgc aggcattggg tctgggtacc gccgctctcg gcgtgacggg
    28861 gatcgcccgc gccgaggaga aggtaatcca gggatttgag aaggaagcga ccgatttgaa
    28921 cgcatccaag gactggaagc ccgtctcgga tcgcaaggtt cgcgtcggta ttgccggata
    28981 tggcgtatgc cggttcgggg cggcgttcag cttccaggac catcccaacg tcgagatcgt
    29041 ggctgtcagc gacctggatt ccgacaaatg cgctgctctg gccaaggccg tccgttgcga
    29101 gaaaacctat ccttcggccg aagagatgat caaggatcag aagatcgaag cggtgttcgt
    29161 cgcgaccgat gccccgagcc acgcgcggct ggccataatg gctctccaac acggcaagca
    29221 cgtgtgctcg gccgtgccgg cgtgcttcgg ttcgatcgag gacgccgaca agctctttga
    29281 ggcggtcaag acaaccggcc tcaagtacat gctctttgag acctcgtatt tccatgccga
    29341 tttgtacgcc atgcgggaga tctacaaggc cggcggcttc ggcaagctgg tttacgccga
    29401 aggagagtat taccactata tggaagagcc gatcgactca tacaaaggtt ggcgcgtcgg
    29461 cctgccgccg cagtggtacc cgacgcactc gaatgcctac tacgtcggcg tgggaggggg
    29521 cagcttcacg gaagtc
//
LOCUS       DAOTPW010000034        29183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1669_length_29183_cov_5.652671, whole genome shotgun sequence.
ACCESSION   DAOTPW010000034 DAOTPW010000000
VERSION     DAOTPW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 29183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29183
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1669_length_29183_cov_5.652671"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="P5316_04810"
     CDS             complement(<1..1506)
                     /locus_tag="P5316_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR84310.1"
                     /translation="MNPHVLVACAAVLSAVPVTPGVGGDVLMNWTSRYGDWSKATETG
                     EVPSNWYKTPANGRVFLRNVPAGNHTFGVSRAFGFGPLVVPNVVVADGRTTLLDMRFH
                     TDYHAIPGDKTLKGSVFTQSFVARGDSIIKAAALFATAPQARSVKYTIHDAGPDGPPV
                     GPTALRPPNLNALVASWCHGQVPVTPGRLYCLRVEGTKGTQLDLRLSDSADPMLPLVV
                     DGKMVPGASLAGWVESDPPGLVSTMVCVEGRINPKPDKERCNAEFGQTFIARGKGLAM
                     IDFRPQVPDADGEVTIEIVVRQGGPKGRGFAHRNVRGPNGSVLQAVWPPGEVRTVSGE
                     QYCIELRNPAGGGFKMSTVAGVLYPSGCLAIDEVEVSHLDADMNIVEYGDDIHPPATP
                     RSRILSGDGLARIEYVAPGDPDVRKLQIRGRIGPHNENWPVSVSENTLLAELDVLPGG
                     TGVFHLFGLENFNTYSYAVYAVDVNGNYSKARVDSSFPGKGPVIPPQATVLN"
     gene            complement(1503..3479)
                     /locus_tag="P5316_04815"
     CDS             complement(1503..3479)
                     /locus_tag="P5316_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84311.1"
                     /translation="MRISISPSPITIVPWFMLFSTPTVCGEEIRGRLYDPTGGWKELF
                     VWTGRVGENRLTGNFTDMGGYFSIGVSEPGTYTVGFSRRFGLGPVISPGVAVEHGKTA
                     YVLLLQSWPLDVRGGKQRLEGHKTYAQSFVSRGECVRRARLDVDEPAGTELIYAVRES
                     RPDGRQIGPDSADGQWPPGQVRLARDKTCFLTVRRKDGQALAVLTTKADKTHMAFADG
                     QEITDAIICGRVVTDRTGIIVDNFKAEGEFFGPCKSGFCQTFVARGTGLAMIDFIPAV
                     GGGRRQTTRYTVRILEDGPGGRQVGPTKLSSGSDMIPSAALGFELRAVLYDRAELPLV
                     PGRKYCIDIVGEPKSFRITTHPGNLMDGEFLMDGRTVSDRCLDATIIEYEPDDCPPPS
                     ARNLRADLAGTAVQLSFDVPADMDVNSAAITRSAGTRTKQLAKLYSCPGRMALYVDRS
                     AEPNQTYVYQVELVDAAGNKSEAVSTRVEVKPLTPESNLLINGDFVASTSASGGLCPG
                     WDTAGLAQNARWSIQSPGKKGDSYAISAWQQWSTYDVVTYQRVPVVKGTTYLLSAMTN
                     RKEPFNDGNVNETTLVGIDPLGGSDASTDSVVWSSAEYAPDKWTLQKVRARAEADTIT
                     VYLRARSLQPGFGMEGLFSKASLKVATDSPGDAK"
     gene            complement(3809..4879)
                     /locus_tag="P5316_04820"
     CDS             complement(3809..4879)
                     /locus_tag="P5316_04820"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HRR84312.1"
                     /translation="MTIEDLLHSPGEWLKTGGNLGDVVVSSRIRLARNLSGFRFLTKA
                     SAAERRDIVRTLCEAIDSADFGVETFYIDVEKADPIDRQFLVERQLISRQHGEGEGSR
                     GVAVGASEQISLMINEEDHLRMQVLRHGLDLDAAWNQIAGIDVALEGLVKFAFHEDYG
                     YLTACPTNVGTGLRVSVMLHLPALKITNEIEKVLRAAKDMRLAVRGLYGEGTEALGDF
                     FQVSNQVTLGRSEEEILTDFKNNVIPSIVRYEQETRKALKKDRLTALDDKIWRAFGIL
                     SNARAISSEEALFLLSHLRLGAVMERIKHIDLSVINDLFLFTQSAHLQKRCGKRLDGE
                     QRSILRADYIRERLAKAQPSTN"
     gene            complement(4876..5361)
                     /locus_tag="P5316_04825"
     CDS             complement(4876..5361)
                     /locus_tag="P5316_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRR84313.1"
                     /translation="MLCQRCKKQTATVHLTDLVQGEKRERHLCEDCANAEGVVVKQNV
                     SINDVLNSFLMSQASIQEMARLRCPECGITFVEFRNQGLLGCPNDYDVFGKALTSIIE
                     RAQDGQTRHTGKTPGQVVRIDPVQQERFRLQRELREAVEREDYEQAAKIRDRLGELQS
                     Q"
     gene            5666..7174
                     /gene="trpE"
                     /locus_tag="P5316_04830"
     CDS             5666..7174
                     /gene="trpE"
                     /locus_tag="P5316_04830"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRR84314.1"
                     /translation="MLKILPTFTDFERLAAGGNTIPVYCQLLADNLTPVTAFSAIAEE
                     SPHAFLLESVVGGENIARYSFLAADPQATFEAKNSRITLDGPGGQSVEESLDPLARLA
                     RTLEGYKAVHLPELPRFAGGAVGYAGYDIVRYYEPLPNPPKDDRGLPDMLFGLYDSMV
                     VFDHVVKTILVISHAHVEIDGVRAGYEKACERVAATVKRLTRPRQTRTGHIDRLGDPT
                     IPFTSNFEAHGFQEAVEACKEYIRAGDIFQVVLSQRLRVKTKADPFDIYRALRVVNPS
                     PFMFFLKSPRVMLVGSSPEIMCRVEDGVITNRPLAGTRRRGRTEEEDKVLEAELKADP
                     KERAEHIMLVDLGRNDVGRVAELGSVELRDVMSIERYSHVMHISSTVTGRLRRGLTAF
                     DAMRATLPVGTVSGAPKVRAMQIIDEFEPTLRGPYAGAVGYVDFSGNMDTCIALRTMV
                     ITPGDVFGKNTVYVQAGAGIVADSQPELEYKETINKAKALLRAVEIAETGFE"
     gene            complement(7315..7626)
                     /locus_tag="P5316_04835"
     CDS             complement(7315..7626)
                     /locus_tag="P5316_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84315.1"
                     /translation="MTSQVTPGQWINVRVTALPKSVAGRKTMVRLFEKDRKVKAERAR
                     LARSRKPSSHRRGGRQWWDRPPRLQIVSTKPGATYRIFGSVDVLRELESLKEYVEISP
                     A"
     gene            complement(8303..9646)
                     /locus_tag="P5316_04840"
     CDS             complement(8303..9646)
                     /locus_tag="P5316_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR84316.1"
                     /translation="MRVLMLGWEFPPFISGGLGTACYGLTKGMSEIGTEVLFVLPRPV
                     EASFATHVTLLTPLGAGSSMYRIEGFKNILFRTVDAGFGVSAYQVAAALAASRQGQPS
                     GFLKVPKGETVGRLGEEAAKLAGTATTQPAFSGGQYEGDMFAEVERYTRLATQVAMQE
                     TFDVVHAHDWMTYRAGIAVAALTGKPLVVHVHSTEFDRSGEHVNQRIYDIERQGMHMA
                     TKVIAVSYLTRNIIISRYGVPPERVEVVYNAIDLNGNSLGITPTAIDRNEKLVLFLGR
                     ITMQKGPEYFLRAAKKVLEVMDNVRFIMAGSGDMVRGIIEMAAGLGIGHKVLFTGFLR
                     GPDVERVFRMADLYVMPSVSEPFGIAPLEAMSNDVPVIISKQSGVSEVLTHALKVDFW
                     DINEMANKIIAVLRHPSLQNTLRQHGRIEVQKLSWADSAKRCVEIYQDALGWSAN"
     gene            10375..11694
                     /locus_tag="P5316_04845"
     CDS             10375..11694
                     /locus_tag="P5316_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84317.1"
                     /translation="MARAGLTRRALLGKSAGLATVGLTTLDAQSAERLLGSNDRISVG
                     IVGAGNRGLALARWISQLATEQNVELTAVCDIWNRARDAGADYIEELTGRRPRKCRYL
                     AEICDLADVDALVVATADFQHAPHTRQAVEAGKDVYVEKPFGCDFEQIKLARDAAKAR
                     GRIVQAGTQGRAEGLPWAARDFIRSGKLGKVSFVEVTNSLFEQRWRIPGSETSLSEKD
                     TDWKEFLSYTPEVPFDARKYREFRLFWPYSTGIFCQFMSHAVDLVNLVLDDVPKAAIA
                     TGGVLVWKDGRSNPDTAHCLVEYLGGCIFSYHMRLGNSANGRTLTFYGTNGTLDLHAG
                     VAYGEGGLGEVVMKPGTHGVPELTVVANRRLPERRQGGVMLKAEPDGDHMVDFFECVR
                     SRKQPRASIDAAFYHALATTMAGMSLRLKTRIEYDMTTDTLLPLNTP"
     gene            complement(11784..13448)
                     /locus_tag="P5316_04850"
     CDS             complement(11784..13448)
                     /locus_tag="P5316_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR84318.1"
                     /translation="MATFLVLQGPDKGRTFRVMSESAVIGRKGDQIPLSDRTVSRRHA
                     EVRPEDGSWVLVDLQSANGTYVNGVRVRDPVRLKHGDQIKLGSTLIIYSGDQSMEKLS
                     GSSIPSDMIDLNTSDLNMDSAILASVGADDSIIIAGPELADAARAWRVMSELTSAIGV
                     IIDPDQLLERVMDVIGEELPIDRGFILLYDKKGEELVPHVVRYRRGVRARKQKITTSH
                     RIIKHVLEHRAAVLCTNAMTDSRFATDAGQDSIHDFGLHSVICAPIMVRDLLLGVIHI
                     DSTATTHTYTEEQLRLMSAIGQMTGLAIQNVRLVQQQMQTARLAATGETVAYLSHYIK
                     NILQGIQSGADLVDIGLARPDLETVSKGWEIVNRNLDRILGLTTNMLAFSKQREPNLR
                     QTNFNKIVQDALSLVQKKAQEHKVVLKVQLEESLPPIPVDADGMHQVALNIINNAIEA
                     VTPGEGVVTVSTRFNTETGIADLIISDNGPGIEHDQIEEIFEPFKSTKGHGGTGLGLA
                     VARKIVREHHGRIVVDSRVGEGTAFIIRLPVQELTRESEETYSTME"
     gene            complement(13973..14902)
                     /locus_tag="P5316_04855"
     CDS             complement(13973..14902)
                     /locus_tag="P5316_04855"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRR84319.1"
                     /translation="MVNYIRLLRPADWIKNVFVFAALLFGKQLENRQAVMLSLLAFVT
                     FCLASSAGYVLNDILDRKRDRLHPTKKDRPLACGTVSVASASAMVAVLVALCVILCWL
                     TLPPAFWLTVGIYLALSWTYSVALKHRMLLDVIVIAMLFVLRAIGGAEAISVEVSPWL
                     LTCTFMLCLFLGFGKRRCEVAMIDNSHAMRGHRPLLVRYTPDLLTHLLSTSAGVTIIT
                     FLLYTLDTTRKSPFEATKQNLIYTLPLVVYGVYRYAMLIELGKGTGPTDLILKDAPFL
                     AAVLLWVLSAWAILYGFPQLGITLPSLTTAPAG"
     gene            complement(15185..16597)
                     /locus_tag="P5316_04860"
     CDS             complement(15185..16597)
                     /locus_tag="P5316_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRR84320.1"
                     /translation="MSMTIGSNAGLYQLLHTMNRLESERAVTMARLSTGRRINRGADD
                     PAGLIALNSLRSELASVDAAISVNTRNQSFLNVADSTLMEIGKLTAEIEGLVVAAQGS
                     TVTTEEKAAYQAQIDANIEAIDRLVNNAEFNGQKIFNGENRINATTDDADAIKDIKVH
                     SRNPSVTGNISLSVTISTAAAFAFSNSSTGYNLASALSGDAVIQVTGKLGTAVITLTS
                     GSDSAAILAAINAEKETTGVSAFAGDSGRLTYASTEKGSEAFVTVSVISGPQAMVTNG
                     GTTKVTGSDAVVSINGQSAMASGTEVYYNGNGLSLSFTLNDDTPDTHTVTVIGGGAVF
                     RLGTDLNSRTALGLQGMNTYELGRADLGYLSSLKSGGANSLIASGSKALAIAKEAGQQ
                     VAVMAGRIGGFNKYQIGSTLNALNVAKEELSDAAGRIGDTDYALELATLERQNIVMEA
                     AVSLLSIANSQARSVLNLLK"
     gene            complement(17044..19767)
                     /locus_tag="P5316_04865"
     CDS             complement(17044..19767)
                     /locus_tag="P5316_04865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRR84321.1"
                     /translation="MRKSLCVSAIISALAVWVLTCALAIGAQEPPGIVPPATTGGGTP
                     RIEIAEPKHDFGKVWVGDKLEHAFNIRNVGDAELKILAVKPKCGCTTPAGYPKTIAPG
                     SSADFLFRLNADRYRGDYSTTIDVETNDPLNKIVHLTLSVHMQHHLEANPSFVLFDKL
                     RPDASAQTTVMLTNKTDKELKLATRGLLPAMQVFTWELKEISPGKTYQLLVSANPPYP
                     GKRNQQTIEIDLGIEGIPPVRIPCTAVVPERLDIEPRMLLVPVDSRPRTRVIAFTNNS
                     NNDVRLLEAVSADSNIAVNVSTLKPGRDYRVNLTFPANYVAPAEGTFITLKTDDTEQA
                     EIKIPVRSPATATRPTGAPPDVFADAQRATLSPQAAETPSDSQPAMQLIGIPAPVINA
                     ATFDGRPLQIGGPAGRVRLLTFYTSWCGYCRQALPGVEDLHKRYKDKGVEVLAINLDV
                     RTGRQPRTEEQVLAHYKELNLSMPMIMDPYQLIGPRFRVRSHPTFVLIGKSGVVEAVH
                     FGHTVPFGSTLPTQIDLLLQGKTRSDFPPAVAEALPPAATQPTATQPAARRDPVMELV
                     GNPAPSATVKTIDGKETKIGAGSERIQLLAFYASWCGFCKKAMPTVEQIHKDYGNKGI
                     EVIAINQDSREGARGRTEDQTLTTYKEWNLSMPMTMDPRQAIGRQFKVRGYPTFVLVG
                     RSGIVEAAHVGAAPTFDSTVRTELDLLLKGKNREAFPQQPAAPVATQPEQRDPAMQLT
                     GKPAPSVSAQTPDGKEIKIGSANGKVQLLAFYASWCGFCKAAMPAVEQLHKEFKDKGV
                     EVIAINQDARSGRGARTQEQSLATYKEWNLSMPVTMDPDHKIGEQFLVRGYPTFFVVG
                     SNGKVEAVYVGGGAVSGGTARSLLDSLLKNKPLTGTPGAGSGAPAASQPAT"
     gene            19954..20946
                     /gene="hemH"
                     /locus_tag="P5316_04870"
     CDS             19954..20946
                     /gene="hemH"
                     /locus_tag="P5316_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRR84322.1"
                     /translation="MIRSESSRGPFDAVLLVSFGGPQGPQDVGPFLDNILRGRPTPPG
                     RVEAIIANYMRYNGVSPITEITRRQAAGVRQRLADAGVELPVYVGMRNWHPYLADVMA
                     EMSRAGVRRAIGFITAAHHSYASCGQYKENVREARRRLAELGLPDVEVTYVESWYDHP
                     GFIGTLARQVRAAIDRLEAPLRPKARIVFTAHSIPVAMAESCRYREQLQTTARMVAED
                     LGRRDWALVYQSRSGPPQDPWLGPDINDYLKAEHEKGLEAVVVSPIGFICDHIEVLCD
                     LDVKVGDLCREIGLPMARAETVNDDPMFLDMMTEVVLRACNRHKRFPPLMITSR"
     gene            21411..21629
                     /locus_tag="P5316_04875"
     CDS             21411..21629
                     /locus_tag="P5316_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HRR84323.1"
                     /translation="MTKAIYADGVFKPEEKLDLPDQQRVQVIIQPIEEQADTNHTAAL
                     DRFFDHVSRSEFSYTGPMPTRDELHDRV"
     gene            22117..23523
                     /locus_tag="P5316_04880"
     CDS             22117..23523
                     /locus_tag="P5316_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRR84324.1"
                     /translation="MRRTRRDFLGKTIRGAASVSLGGSLLSAGGCFRSEPPDGRIHLR
                     YWDKWDGFEGDAMRAVVKDFNDSQGGITVHYTPVGVIDRKLLAATSGGDPPDVAGLWE
                     HNVTPFAEQGALTPLDELMSRDGIGRDHWIAAYVKICTHKGIMWAVPTTPFASGLHYN
                     AALFEASADQLRARGLDPGRGPATLAELDAYAEALTKYDASGNLVQLGFLPQEPGRFI
                     WAWGFWFGGKLIGEDDTITADDPLNVAAYEWIQSYSRKYGVDRIKRFSSGFGNLASPQ
                     NPFFSGKVAMVLDGAWMHNYIQQYAPGMRYGVVPWPKTPHGPERFSTVGCDIMVIPRG
                     VPAERREAAWQFVKYVISQPAMEKLNLGHRKATPLTKVSDDFLKKHPHRYLDLFISLS
                     KSKGVVSPLHVGIWSQYQTELFAAFERVRLLQSNPATGKPYTAREALDIVQERMTRAY
                     ARYKRSLSLRPPISGRLS"
     gene            23520..24431
                     /locus_tag="P5316_04885"
     CDS             23520..24431
                     /locus_tag="P5316_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRR84325.1"
                     /translation="MTRADRRHLVNGILFASPWLIGLSVFLAYPILSSLYYSFCDYST
                     LRPAVWVGLQNYQDLGQDAVMFKSLRNTLVFGAIALPGSLTAALMLALLLNSGVRGLP
                     IFRTIFYLPCLVPAVASAMLWLWVLNGDFGILNYALGALLGPLGLRPPTWLADPLWAK
                     PALAIMSIWGCGGTMVIYLAGLQDVPAELYEAAEIDGAGWWRRLFHVTLPCLSPVIYF
                     TLIIGIIAVLQVFTPAYIMGGGPQGGTQGQPAQSTLFYALYMWAQAFEWLRMGYGCAM
                     AWILFVIILVLTLIAHRTAGKRVTYVG"
     gene            24438..25640
                     /locus_tag="P5316_04890"
     CDS             24438..25640
                     /locus_tag="P5316_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRR84326.1"
                     /translation="MEPRTLFGKVLAYALLVLGSFLFIFPFIWLLSTSLKPLEQSTAM
                     PPQWIPRQYRATIDGRECEVVRGETLAARMLVVRLDIEDETDPRGGQKLLVPESDYQD
                     GTIPLKVAGIEQRIPVTVLRRAEPGWCYIKERKTLVDMNAPAVWDIVPPEAVESRAKL
                     FWGNFPAAINKMGTARVWMPLLGEREVSIFWLYLRNTLIVCLLGVTGTVCSSSLAAYS
                     LAKIPWRGRGVLFGLTLATMMVPFAVLMVPLYTLFARFGWIGTLLPLWVPAFFGSGFN
                     IFLLRQFFLTIPKDLSEAARIDGCSEFGIFWRVILPLSKPALAVVALFHFLYAWNDFM
                     GPLLFLTRRETFTLSLGLQAFQSQHGGTDWTSLMAASTLICLPIIVLFFFTQKTFIKG
                     IATTGLKG"
     gene            complement(26145..28160)
                     /locus_tag="P5316_04895"
     CDS             complement(26145..28160)
                     /locus_tag="P5316_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HRR84327.1"
                     /translation="MAERKGSSAAGAPTGIVREVPPLQENATPEEKELHWYRHVYQGE
                     RVRQLTLRAVLTGGILGMFMCVSNLYTTLKLGWSFGVAITACVLSYVIWNAVRAVSGN
                     RLSEMTILENNCMQSTASAAGMSTGGTLATAVGSLLLMSGTHLPWTTLVAFTFLTAAL
                     GVFMAIPLKRQMINIEQLPFPSGMAAAETLRSLHSRGAEAVRKAYSLIIALGCGAVTG
                     FFRGPEGVITTLDKIYRYVHIPELLPFGGIFNPMSWLRQGAAKAGLAGLGFEPGTLMI
                     AAGAFVGTRVSFSMLAGSILLYYVTMPLLVSHGAQVAVAGSSGYVPAGDLPPGAILLP
                     TRWALWGGTSIMVFSSLVSVGLQWRTLARSFRVFRRTTHADSGQTVRDSRGSSVDLQK
                     AIDAIEVPSSWLIGGLIPIGVAIVLLNYFAFHMSILLGVIAVAMSFVLSLVACRATGE
                     TDTTPTGAMGKVTQLVYAILAKGNYVVNLMSAGTTAAAAGSAADLLTDLKSGYLLGAN
                     PRKQFLAQFSGIFFGVAAIIPAWYLMVPTAERLHAFNPPAVYMWKAMADALTQGLHTI
                     PITAQWAILIGAVLGIILPVLSAKLPKAAPYLPSAMGLGLAWVVQFQNSLSFAIGAVI
                     AWAWARTHKKSAETYTFPVAAGMIAGESIMLAMIAIAATATQLFGHQ"
     gene            28355..>29183
                     /locus_tag="P5316_04900"
     CDS             28355..>29183
                     /locus_tag="P5316_04900"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84328.1"
                     /translation="MVNVVDRISGDGTFRALVARIRELSPLAAGSGDETHSPSGAQGG
                     VVSVDGLWGSFAPVLAGAAALELKCPLLYVTAHLDPADEIRDDLELCCGRTPELLSAF
                     EAAPGQGAASDEIYAERIRLCAQLQRASQGDQEPPSFIVAPIQALMQCVPSERALAGQ
                     LLTLCVGQEHDLQRIARWLMDHGYERLDQVESPGDFALRGDILDIFPPAQTDPYRIDF
                     YGNQIESIRGFDVSTQRSDTEFETIQIAAASVRGRQDHSPHKHQTVEARGVVSQRSHP
                     "
BASE COUNT         5419 a         8556 c         9255 g         5953 t
ORIGIN      
        1 gttcagcacg gtggcttgtg gcggaatcac aggccccttg cccgggaacg aggaatccac
       61 gcgggctttc gagtaattcc cgtttacatc cacggcgtag actgcgtagg aataggtgtt
      121 gaagttctcc aggccgaaga gatgaaacac accggttccg ccgggtaata catccagttc
      181 ggcaagcaag gtgttctcgg agacgcttac cggccagttc tcgttatggg gaccgatgcg
      241 gccgcgaatc tgcagcttgc gaacgtccgg gtcacctggg gcgacgtact caatcctggc
      301 caggccgtcg ccggacagga ttcgagaacg cggagtagca gggggatgga tatcgtctcc
      361 gtattcgaca atattcatgt ctgcgtcgag atgtgagacc tcaacttcgt caatcgccag
      421 gcagccgctg ggatagagga cgcccgccac cgtggacatc ttgaagccgc ccccggcagg
      481 gttgcgtaat tcgatgcaat actgttcgcc ggaaacggtc ctgacttcgc ccggcggcca
      541 gacggcttga agcacggaac cgttggggcc gcggacgttc cggtgggcga atcctcttcc
      601 cttggggcct ccttgtcgga cgacgatttc gatcgtgact tcgccgtcgg cgtcgggaac
      661 ctgggggcgg aagtcgatca tcgcgagtcc tttgccccgg gctatgaatg tctggccgaa
      721 ctcggcgttg cagcgttcct tgtcgggttt tgggttgatc ctgccttcaa cacagaccat
      781 ggtcgagaca aggccgggcg gatctgactc aacccagcct gccagagacg cgccgggaac
      841 cattttgccg tcgaccacca acggcagcat cgggtcggcg gagtcgctga gccggaggtc
      901 aagttgcgtt ccttttgtac cttcgacgcg gagacagtag agccgcccgg gtgtcaccgg
      961 gacttgtccg tgacaccacg aagccacgag agcgttcaga ttgggaggcc gaagagcggt
     1021 cggccccacc ggcgggccgt cggggccggc gtcgtgtatc gtgtatttca cgctgcgggc
     1081 ctgcggcgcg gtcgcgaaca gcgccgccgc ctttatgatc gaatcacctc tggccacgaa
     1141 ggactgagtg aacaccgagc ctttcagtgt tttgtccccc ggtatcgcat ggtaatcggt
     1201 atgaaagcgc atatcgagca gagtggttct gccatccgcg acaaccacgt tcggcacgac
     1261 cagcggtccg aagccaaacg ctcgagagac gccgaaggtg tggtttccgg ccggtacatt
     1321 tctcagaaag acccgaccgt tggccggcgt cttgtaccag ttggacggga cctcgcccgt
     1381 ctccgttgcc ttcgaccagt caccataccg gctggtccag ttcatcagta cgtcaccgcc
     1441 gacccctggc gttaccggga cagcactcag cactgccgcg cacgcgacga gaacgtgtgg
     1501 gttcatttcg catctccggg agaatcagtc gcgaccttga gactggcctt cgagaacaag
     1561 ccttccattc cgaagcctgg ttgcagcgag cgagcccgca agtacaccgt gatcgtgtcg
     1621 gcctcggctc ttgcgcggac cttctggagc gtccatttgt caggtgcata ctcggccgac
     1681 gaccagacga cggaatccgt tgaggcatcg gaaccgccaa gcggatcgat tcccacgagg
     1741 gtcgtctcgt tgacattacc gtcgttgaac ggctctttac ggttggtcat tgcgctcaag
     1801 aggtatgttg tgcccttgac caccgggact ctctggtatg tcacgacgtc ataggttgac
     1861 cattgttgcc aggcgctgat ggcgtacgaa tcccctttct ttccgggcga ttggatcgac
     1921 cagcgggcgt tttgagccaa gccggccgta tcccatcccg ggcaaagacc gccagacgcg
     1981 gacgtgctgg cgacaaagtc gccgttgatc agcagattgc tttcaggcgt cagcggcttc
     2041 acttccaccc gggtgctgac ggcttcggac ttgttgcctg cggcatcgac gagttccacc
     2101 tggtacacgt atgtctgatt cggctcggct gagcgatcca catagagggc catgcggccg
     2161 gggcacgagt agagctttgc cagttgcttt gtcctggtgc ctgcggaacg ggtaatggcc
     2221 gcgctgttga cgtccatgtc ggcagggaca tcgaaagaga gttgcaccgc tgttcccgcc
     2281 aagtccgcgc ggagattacg cgccgaaggg ggcggacagt catccggctc atactcgatg
     2341 attgtggcat ccaggcaacg gtccgatacc gtccggccat ccatcaggaa ctcgccgtcc
     2401 atgaggttgc cgggatgcgt cgtgatgcgg aagctctttg gctctccaac aatgtcgatg
     2461 cagtacttgc ggcccgggac cagcggcagc tctgcgcggt cgtacagcac ggcacgaagc
     2521 tcaaatccga gggctgcgct tggaatcatg tcgcttcccg atgaaagttt cgtggggccc
     2581 acctgcctac ctccggggcc gtcttccagg atccggacgg tgtatcgagt cgtctgtcgg
     2641 cggccgccgc caaccgccgg gatgaaatcg atcatagcca ggccggttcc gcgagccaca
     2701 aaggtttgac agaaacccga cttgcacgga ccgaaaaact cgccctcggc cttgaaattg
     2761 tcaacgatga tacccgtccg gtcggtcaca acgcgtccgc aaatgatcgc gtcagtaatc
     2821 tcctggccgt cggcgaatgc catgtgggtt ttgtcggcct ttgtcgtgag gacggccagt
     2881 gcctggccat ccttgcggcg aacggtgagg aagcaggtct tgtcgcgggc aagacggact
     2941 tgtcccggcg gccattgccc gtcggcacta tcaggcccta tctggcggcc gtcgggacgg
     3001 ctttcgcgca cggcgtagat cagttcggta cctgcgggtt cgtccacgtc cagccgcgct
     3061 ctgcggacac actcgcctcg tgagacaaag ctttgagcgt aggttttgtg accttcaagt
     3121 cgttgtttgc cgccgcgaac atccaaaggc cacgattgca gcagaaggac ataagccgtc
     3181 ttgccgtgct cgaccgctac tccgggtgag atcaccggac caaggccgaa gcgacgcgag
     3241 aaaccgaccg tgtaggtgcc cggttccgaa acgccgatgc tgaagtagcc gcccatgtcg
     3301 gtgaaattgc cggtgaggcg gttttctcca acccggccgg tccaaacgaa caactccttc
     3361 cacccgcctg tgggatcgta caagcgccct cgaatctcct cgccgcatac ggtcggcgtc
     3421 gagaacagca tgaaccacgg aacaatcgtg atcggcgacg gagaaatgct tatgcgcatg
     3481 gcttgaacct cgatgccacc tgaagtaccg agagaaccac tgaacgcggc gccccaatga
     3541 ataccatcca accgcagatc tcgtcaacaa accgccccgt cgaaggcgca gaaacacaac
     3601 cgccaccgat cgactgcgaa gacggggccc atgttcggga tacgctgctg agcggaacgt
     3661 accgagctcc gtcggggcga gccgccatct ctcggcaaag gcaaccgcag tcgaattgct
     3721 cgggaggagg cgatcctcgc acgcaagccg catggcaatc aaagcggcgc ctgcttgccc
     3781 ggcggttttg gctatccgga tgcttttttc aattcgtact tggttgggcc ttggccaggc
     3841 gttcgcggat gtaatcggcg cgcagaatgc tgcgctgctc accgtcgagc cgcttcccgc
     3901 atcgcttctg caggtgggct gactgggtga agaggaagag atcgttgatc acgctgagat
     3961 cgatgtgctt gatgcgctcc atgaccgcgc cgagtcgcag gtgtgacagc aggaacaacg
     4021 cctcctcgct gctgatggcc cgtgcattgg agagtatgcc gaacgcccgc cagatcttat
     4081 catcgagggc ggtcagccga tccttcttga gagcctttcg ggtttcctgc tcgtagcgga
     4141 cgatggacgg gatcacgttg ttcttgaaat cggtgaggat ttcttcctca cttcggccga
     4201 gggttacctg gttggaaacc tggaagaagt cgcccagggc ctcggttccc tcaccgtaca
     4261 aaccgcggac cgccagacgc atgtccttgg cggcgcgcag aaccttttcg atttcgttgg
     4321 tgattttgag ggccgggaga tgcagcataa ccgagacccg cagaccggta ccgacgttgg
     4381 tcgggcaggc cgtcaggtag ccatagtcct cgtggaaggc gaacttgacg agcccctcca
     4441 gagcgacatc gatgccggca atctgattcc aggcggcgtc aagatccaac ccgtgccgga
     4501 gcacctgcat ccgcagatgg tcttcttcat tgatcatcag cgagatctgt tcgcttgctc
     4561 cgaccgccac cccacggctt ccctcgcctt caccgtgctg ccggctgatg agctgccgct
     4621 cgaccaggaa ttgtcgatcg atcggatcgg ctttctcgac gtcgatatag aaggtctcga
     4681 cgccgaaatc agccgagtcg atggcctcgc agagtgtccg gacgatgtcc cgacgttcgg
     4741 cggcactggc cttggtcaga aagcggaagc cgctcagatt gcgggccaat cgaatgcgcg
     4801 acgaaacgac gacgtcaccg aggtttcccc cggtcttcag ccattcgcct ggcgagtgga
     4861 gaaggtcttc aatggtcatt gtgactgcag ttcccccagc cggtcgcgga tcttggcggc
     4921 ctgttcgtag tcctcacgtt ccaccgcctc gcgcagctcg cgctgaagcc ggaatctctc
     4981 ctgttgaacc gggtcgatcc ggaccacttg gcctggtgtc ttacccgtgt gtcgcgtctg
     5041 accgtcctgt gcccgctcga tgatgctggt caaagctttg ccgaagacgt catagtcgtt
     5101 tgggcaaccc aacagaccct ggttgcgaaa ctcgacgaaa gtgattccgc attcggggca
     5161 tctgagacgg gccatctcct ggatgctggc ttgggacatc agaaacgaat tgagaacgtc
     5221 gttgatcgag acgttctgct tcaccacgac gccttcagcg ttggcacagt cttcacagag
     5281 gtgccgttct cgcttctcgc cctggaccag atccgtcagg tgaacggttg ccgtctgttt
     5341 cttgcaccgc tggcacaaca tacccagtac ctgtcttgca atacccggga ggacgcttca
     5401 atgttaggcg tcttcatata tatcgtcaag gcaaagcgga cccttcaggc ccttctcccc
     5461 gtcctgtcaa ggacttgcga cacggatcgc cggcgggtgg cggccttgcg ggtggtccgg
     5521 cggctgccgg gcacaagcat gcgggcatcg tgccttggcc tgcgtccggc ccggtcatat
     5581 tctacggcaa ccggcaacct gagccgtctg aatgacgtcg tccacaccgt tgaccgctga
     5641 ctacctcaag ggatactatg acgcgatgct gaaaatcctg ccgactttca cggacttcga
     5701 gcgtttggcc gccggcggca acaccatccc ggtgtattgt cagttgctgg cggacaatct
     5761 gacccctgtc acggctttct ccgctatcgc tgaggagagc ccacatgcgt ttctgctgga
     5821 aagcgtggtc ggcggagaga acatcgcccg ctactcgttc ctggcggctg atccgcaggc
     5881 gaccttcgag gccaagaaca gccgaatcac gctggacggt cccggcggcc agtctgtcga
     5941 ggaatccctt gatccgttgg cacggctggc ccgtaccctg gaaggctaca aggcggtcca
     6001 tttgccggag ttgcctcgtt tcgccggcgg ggccgtgggt tatgcgggat acgacatcgt
     6061 gcgctactac gagccgctgc cgaacccgcc caaagacgac cggggcctgc cggacatgct
     6121 ttttggcttg tatgacagca tggtggtttt cgatcatgtg gtaaagacca tcctggtgat
     6181 cagccacgcc cacgtcgaga tcgacggggt tcgagccggt tacgagaagg cctgcgagcg
     6241 cgtcgccgcg acagtcaaac ggctgacccg gccccggcag acccgaaccg gccacattga
     6301 ccgtctgggc gacccgacaa ttccgtttac gagcaacttc gaggcgcacg gctttcagga
     6361 ggccgtcgag gcgtgcaagg aatacatccg ggcaggggac attttccagg tagttctgtc
     6421 gcagcgactt cgcgtgaaga ccaaggctga cccgttcgac atctaccggg ccctgcgggt
     6481 agtcaatccc tctcccttta tgttctttct gaagagcccc cgggtcatgc tggttggttc
     6541 atcgccggag atcatgtgcc gggttgagga tggtgtcatc acgaatcgtc cgttggcggg
     6601 gacccggcgt cgcggtcgaa ccgaagagga agacaaggtc ctggaagccg aactcaaggc
     6661 agaccccaag gaacgggcgg agcacatcat gctggtcgac ctcggtcgaa acgacgtggg
     6721 gcgggttgcc gaattgggta gcgtggaact ccgcgacgtc atgtcgatcg agcggtacag
     6781 ccacgtcatg cacatcagca gcacggttac cggccgactg aggcggggct tgactgcttt
     6841 tgacgccatg cgggcgacgt tgcccgtggg cacggtcagc ggagccccca aggtccgggc
     6901 tatgcagatc atcgacgagt ttgagccgac gctccgcgga ccctatgccg gggcggtcgg
     6961 ttacgttgat ttctcgggta acatggatac ctgtatcgcg ttgcggacca tggtgatcac
     7021 gcccggtgac gtgttcggga aaaacaccgt ctatgtgcaa gccggcgcag gaatcgtggc
     7081 cgactcacaa cccgagctcg aatacaagga aaccatcaac aaggcgaaag ccctgctccg
     7141 ggccgtcgaa atcgcggaaa cggggtttga atagaccgac caggataccc ggtcgccttg
     7201 tccggaccga aggccaaccg ggagaaaaac ccggactctg ccagtgcttg ctgattgacg
     7261 ggggctcaca caggcagagt ccgaggttat gatttctgtt tcgcgaccgg accgtcaggc
     7321 cgggctgatc tcaacgtact ccttgagact ttccagctct cgcaggacat cgaccgaacc
     7381 gaagatcctg taagtggcgc caggcttcgt ggaaacgatt tgaagccggg ggggacggtc
     7441 ccaccactga cgaccgcctc gccgatggga tgagggttta cgggaacgag cgagccgggc
     7501 gcgctccgcc ttgaccttgc ggtccttttc gaaaagtcga accatcgtct tacggcctgc
     7561 caccgatttc ggcagggcgg tgactctcac gttaatccat tgaccgggcg ttacttgcga
     7621 cgtcattgcc gactcccaat tgcacgttcg gtttcggttc gcgaccgccg tttggccggt
     7681 cgatagcggt ttccgaaccc ggaaaacaac cagtatagcc gttgtgggtc tgattgccaa
     7741 gatcggtgcg acgggggctt ggccgacggg tgcgtgacag aagcggtggc actcctcgcg
     7801 gctgacccgg ttgtcgtgaa cgcaagccga aagcagacca tttcagcggt cttaagggca
     7861 gataggccgg cctgtttatg gccggcttgc ggccaggcaa tcattcctct tctctccgtt
     7921 tcctcccatc cagcccgttt taaggggcct gtccttgtcc ggtaagcccg ttgaaacggg
     7981 ctgagaccat aaggaaggaa gaagaaccgc aggcgaccac caaccggccg tatgagggcc
     8041 gggctttggc gggaaccccg ctgaacgggg tcgatcgccc aacctggacc ggtcggcata
     8101 cccgtccagg ctgtcacgca cccttggcca actcggtggt ggttcgaaca gccctctcgg
     8161 aacacagggt gcttcggcga ccatcccgaa ggtacggtca tgttaaaatg ggcagaataa
     8221 gatctttgtc gttgtaggcc gatgggcggg tctgactcgg tcgcgtggta aagggacgct
     8281 ggacggcgga ttgggtacga cgtcagttgg ccgaccagcc caaggcgtcc tggtagattt
     8341 cgacgcatct cttggctgag tccgcccaag acagcttctg gacttcgatt cgtccatgtt
     8401 gccgcagcgt gttctgcagt gacgggtgcc ggaggaccgc gatgatctta ttggccatct
     8461 cgttgatgtc ccagaagtcg actttcagcg cgtgggtcag gacttcagag actcccgatt
     8521 gcttgctgat gatcaccggc acatcgttgg acatggcttc gagcggggcg atgccgaacg
     8581 gttcggagac gctgggcatg acgtaaagat cggccatgcg aaagacgcgc tcgacgtcgg
     8641 gaccgcggag aaagccggta aacaggactt tgtggccgat tcccaagccg gcggccatct
     8701 cgatgatgcc gcgaaccatg tctcccgagc cggccatgat aaagcggacg ttatccatca
     8761 cctccagaac cttcttggcg gctcggagga aatactcagg gcctttctgc atggtgatcc
     8821 ggcccaggaa aagaacgagc ttctcgttgc ggtcaatggc ggtgggggtg attcccagcg
     8881 aattgccgtt gaggtcgata gcattgtaca cgacctcaac gcgctcgggg ggcacgccgt
     8941 agcgggaaat gatgatgttc cgggtgagat agctgacggc gatgaccttg gtcgccatgt
     9001 gcatgccttg gcgttcgatg tcgtagattc gctggttgac gtgctcgccg ctgcggtcga
     9061 attccgtcga gtgaacgtgc accaccagag gcttacccgt tagggctgcg accgcaatac
     9121 ccgcgcggta ggtcatccag tcgtgggcat ggaccacgtc gaacgtttcc tgcatggcca
     9181 cttgtgttgc caagcgcgtg tatcgctcga cctccgcgaa catgtcgcct tcgtactgcc
     9241 cgccgctgaa agcaggctgc gtcgtggccg taccggccag ctttgcagct tcttcgccaa
     9301 gccgcccgac ggtttctccc ttgggtacct tcaggaatcc tgaaggctgt ccctgtcggg
     9361 aggcggccaa tgccgcggcc acctgataag cggacacacc gaacccggca tcgaccgtcc
     9421 ggaagaggat gttcttaaat ccctcgatgc gatacatgga tgagccggcg cccagggggg
     9481 tcaagagggt aacgtgcgtc gcgaagctgg cctccacggg tcgaggcagc acaaacagga
     9541 cttccgttcc gatctcgctc atacctttgg tcagcccgta gcaggcggta cccaatcctc
     9601 cagagatgaa tggaggaaac tcccaaccaa gcatcaggac tctcatggct cagatactcc
     9661 catcggagcg acaggatcgt agttttggac caacctcacg cacggagctt caaacgcccc
     9721 aaacagaagg gacacgggca agtgcgggcc gacatcctgt ccctcgattg agagacgagt
     9781 ctcattctcg aggaagacgc cgcagcgcac gcgccatggt cctcactgtc catgctgcta
     9841 ccgcgcctga cagcacaccc aaatggcccc ggcgtcggca gaacctatca cctctatccc
     9901 cgccggtctt gagagtcccc gaaccttcgc gccatattaa ggtgcgcctc gacgcttcgg
     9961 tccggtttcg agacagccgt gggcagggag cgccggatcg cggcaccgcc cttctgggct
    10021 gttgtgccga gccgcctgga cggtggtgct tgatcggccg ctccgcagtc catcgaccgg
    10081 aataagcata tccccggttc atcggagtgt caaacggttg ccgcacaaag tacgtctgtt
    10141 ctgaaggttg ccgatcgcgg tgagcgttga aaggacggac atccacgaca ttccattcgc
    10201 atcggcgtcg ctttgtccgc ttcccggctg tcaggacctc gggcggggga tagcggttcg
    10261 gaacggactg cgtttttttc caaaaccggg tcgcgagttg tagaatgacc tgctgggttc
    10321 gttggacgac tgaattcacg gcgtggcgct gaccgcaaca gggggatagg ccctatggca
    10381 agagcaggct tgacccgacg tgcgctgctt gggaaatcgg cggggctggc aactgtcgga
    10441 ttaacgacac ttgatgccca gtcggccgaa agattgctcg gttcgaatga tcgcatttcc
    10501 gttggcatcg tcggagccgg caatcggggt ctggccttag ccaggtggat cagccagctc
    10561 gcgaccgagc agaacgttga actgaccgcc gtctgcgaca tctggaaccg cgctcgtgac
    10621 gccggggcgg actatattga ggaacttacg gggcgccggc cgcgcaagtg caggtatctg
    10681 gctgagattt gcgacttggc agacgtggat gccttggtcg ttgcgactgc cgacttccag
    10741 catgccccac atacccgtca agccgttgaa gcgggcaagg acgtgtacgt agagaagccg
    10801 ttcggctgtg attttgagca gataaagctc gcgcgcgatg cagcgaaggc ccgggggcgg
    10861 attgtccagg cgggtaccca gggtcgggcg gagggtctac cctgggctgc cagggacttc
    10921 atccggagcg gtaagctagg aaaggtttca tttgtggagg tgacgaattc gctctttgaa
    10981 cagaggtggc ggattcccgg ctcggagacc tcgctcagcg aaaaggacac ggactggaaa
    11041 gagtttctct cgtatacgcc tgaggttcct tttgatgctc gcaagtaccg ggagtttcgg
    11101 ctcttttggc cctattcgac ggggatcttc tgtcagttca tgagtcacgc ggtggacttg
    11161 gtcaacttag tgctggatga cgtgccgaag gccgcgatcg cgaccggggg ggttcttgtg
    11221 tggaaggacg gccggtccaa tcctgacacc gcccactgct tggtggagta ccttggcggc
    11281 tgcattttca gctaccacat gcggctgggc aattcggcga acgggcgcac cctgaccttt
    11341 tacggcacca acggaacctt ggatttgcat gccggagtcg cttatggcga gggtggcctt
    11401 ggcgaagtgg tgatgaagcc cgggacgcat ggtgtgccgg agttgacggt tgtcgccaac
    11461 cggcgacttc cggagcggag gcagggggga gtcatgttga aggccgagcc ggacggcgat
    11521 cacatggtgg atttcttcga atgcgtccgt tcgcggaagc agccccgggc ctcgatcgac
    11581 gctgcgttct atcacgccct ggctacaacg atggccggca tgtctctgcg actcaagacg
    11641 cggatcgaat acgacatgac cactgacaca ctcctgccgt tgaacacgcc gtagctcttt
    11701 gccggttgtc ttgccctgtt tgaatgccgt ggcgcaccgg tatccgcgca gccgcctggt
    11761 ttgcgtgagg ttggacagca tctctactcc atcgtggagt aggtctcttc gctttcgcgg
    11821 gtgagttcct gaaccggcaa gcggatgatg aacgcggttc cttcgccgac gcggctgtcg
    11881 acgacgatcc gcccgtggtg ctcgcggacg atcttgcgag ccaccgccag cccgaggccg
    11941 gtgccgccat gccccttggt cgatttgaac ggctcaaaaa tctcctcgat ctggtcatgt
    12001 tcgatgccgg gcccgttgtc cgaaatgatc agatccgcga tgccggtctc ggtattgaac
    12061 cgcgtgctga cggtgaccac gccttctcct ggtgtgacgg cctcaatggc gttgttgatg
    12121 atattgaggg ccacctgatg cattccgtcg gcgtccaccg gaatcggggg cagtgactct
    12181 tcgagctgga ccttgaggac gaccttatgt tcctgggcct ttttctgcac cagcgacaag
    12241 gcgtcctgaa cgatcttgtt gaagttcgtc tggcgaaggt tgggttcgcg ctgcttgctg
    12301 aaggcaagca tgttggtggt caggccgagg atccggtcga ggttgcggtt gacgatctcc
    12361 cagcccttgc tcaccgtttc caggtcgggc ctggcgaggc cgatatcaac caagtcagcc
    12421 ccgctttgga tgccttgcag gatgttcttg atatagtgcg acagataggc gacggtttcg
    12481 ccagtggcgg cgaggcgggc ggtttgcatc tgctgctgta ccagccggac gttctggatg
    12541 gccaggccgg tcatctggcc gatggcgctc atgagccgaa gctgctcttc ggtgtaggtg
    12601 tgggtggtgg ccgtgctgtc gatgtggatg accccaagca gaaggtcccg caccatgatg
    12661 ggggcgcaga tcaccgagtg cagcccgaaa tcgtggatgc tgtcctggcc ggcatcggtg
    12721 gcgaaccgcg agtcggtcat ggcattcgtg cacaggacgg cagcgcggtg ttcgaggacg
    12781 tgcttaatga tccggtgcga cgtggtgatc ttctgtttgc gggcccgcac gccgcggcga
    12841 taccgcacca cgtgcggcac cagctcctct cccttcttgt cgtaaaggag aatgaagccg
    12901 cggtcgatcg gaagctcctc accgatcacg tccatcacgc gttccagcag ttgatcaggg
    12961 tcgatgatga cgccgatggc gctggtcagc tccgacatca cgcgccaggc acgggcggcg
    13021 tcagccagtt cagggccggc gatgataatg gaatcgtcgg ctcccacgct ggcaaggatg
    13081 gccgagtcca tgttgaggtc gctggtattc aggtcgatca tgtcggacgg aatactggat
    13141 ccgctgagct tttccatcga ctgatcgccg ctgtagatga tgagcgtcga gccgagcttg
    13201 atctgatcgc cgtgcttgag acgaaccggg tcgcgaacgc gcacgccgtt gacgtatgtt
    13261 ccgttggcgc tttgcaggtc gaccaggacc caactgccgt cttccgggcg gacttccgcg
    13321 tggcggcgcg acacggtccg gtccgacagc gggatctggt cgcccttgcg gccgataacc
    13381 gcggactcgc tcattactcg gaaggtgcgt cctttgtcgg gtccctgaag gaccagaaac
    13441 gtcgccacga tcaactcctg accacaatca gcttcgcact atgtgcgatc ctcgactttt
    13501 tcctgaaggc gccaaggcct tcggtcgccg gcctgacccc gcaacccgcc gtgggcttct
    13561 cgcggcggac gcggaacccg agaaccaact tcatgactcc gccaagaccg aagtagctcg
    13621 cgcccgaagg gacgaagaaa ggcggtcact gcccggtccg ctacaggtcg gcatcagcga
    13681 ccttccatct tgattctatt ctttgaatcg gggcagtcaa atgggccaac cgccgtgtct
    13741 ggggggcggg ggtcggtgac agcgcggacg actctttcag aatgtccgag cttgaatgag
    13801 cggccaagcc gccaagcggt caacaccgca cgggaaccgc tcccttgcag cttggtcagc
    13861 gggccgcgcc gggcaggcac gggcgtgttg ccgaacgagg gtcccggttc tgaaagtacc
    13921 ctcaggtttc cctcggccaa gccgtcggaa aagtggcgat gcgtgtggtc aactagccgg
    13981 ccggggcggt cgtgaggctc gggagagtga tgccgagttg agggaagcca tagagaatgg
    14041 cccaggccga gagcacccag agcagaacgg ccgccagaaa gggggcgtcc ttaaggatca
    14101 ggtcggttgg tcccgttccc ttgcccagct cgatcagcat cgcatagcgg tagacgccgt
    14161 agaccaccag cggcagcgtg tagatgaggt tctgcttggt ggcctcgaaa ggcgactttc
    14221 tcgtggtgtc gagcgtgtac aggagaaagg tgatgatagt tacgcccgcg gaggtgctca
    14281 acaggtgggt gagcaaatcc ggcgtgtacc ggacgaggag gggccggtgg ccgcgcatgg
    14341 cgtggctgtt gtcgatcatc gctacctcgc agcgtctctt gccgaatcca aggaacaggc
    14401 agagcatgaa cgtgcatgtg agcagccagg gcgatacctc gacgctgatg gcttccgcac
    14461 cgccgatggc ccggagaacg aacagcatgg cgatgacgat gacgtccagc aacatccggt
    14521 gcttgagagc cacggaatac gtccagctca aggccaagta gatgcccacg gtcaaccaga
    14581 atgctggtgg aagggtcaac caacacaaga tgacgcacag ggcaaccaac accgcaacca
    14641 tggcggatgc cgaggccacg gatacggtcc cacaggccaa cgggcgatct ttcttggtgg
    14701 ggtggagacg gtcgcgcttg cgatcgagga tgtcgtttaa cacatagccg gccgaggaag
    14761 ccagacagaa cgttacgaag gccaagagcg agagcatcac cgcctggcgg ttttccaact
    14821 gcttgccgaa cagcaatgcg gcgaaaacga agacgttctt gatccaatcg gcgggccgca
    14881 gtagccggat gtagttgacc atggcaagta gtgtagaaac cggcaggctg gatgcaactt
    14941 ttctgtggtc gggaaaacgc gaaaggccgt gtggcttgtg ttgccaccgg cctttcggta
    15001 cttctctcct tcgagttgac tctggccggg gcatttcccc cgcaccccgg ctatccgccg
    15061 tgccgaccgc tctcccctct tgggccggca agacggcagc acccctccgg aaagcaagcc
    15121 tgtctggtcc ccatttccgc cggtctcggc ttggcttgaa cgcctgccgg ctaccggaac
    15181 gcgcctattt cagcaagttc aacacgcttc tggcctgaga attcgcgatg ctcaggagcg
    15241 agacagccgc ctccatgaca atattctggc gttcgagtgt cgccagttcg agggcgtagt
    15301 cggtatcccc gatccggccg gcggcgtcgg acagctcttc cttggctacg ttcaaagcgt
    15361 ttagcgttga tccgatctgg tatttgttga acccgccgat gcggccggcc atgaccgcaa
    15421 cctgttgacc ggcttccttg gcgatagcga gcgccttact gcctgaggcg atcagggagt
    15481 ttgcacctcc gctcttgagc gagctgaggt atcccagatc ggcccggccc agttcatagg
    15541 tgttcattcc ctggaggcca agggcagtcc gcgagttgag atccgttccc agcctgaaga
    15601 cggcgcctcc gccgatcacc gtgacggtat gggtgtcggg cgtgtcgtcg ttgagcgtga
    15661 agctgaggct aagtccattt ccgttgtagt aaacctcggt gccggaggcc atcgcgctct
    15721 gaccgttgat gctgaccacc gcatccgagc cggtgacctt cgtcgtgccg ccgttggtga
    15781 ccatggcttg gggcccggag atgaccgaga cggtgacgaa ggcttccgag cccttttcgg
    15841 tggatgcata ggtaagcctg ccgctgtcgc cggcgaaggc gctgacgccc gtcgtttctt
    15901 tctcggcgtt gatggccgcc aggatggcgg cgctgtccga tccgctggtc agcgtgatga
    15961 cggctgttcc gagcttgccg gtcacctgga tgacggcgtc acctgacaaa gcgcttgcca
    16021 gattatatcc ggttgacgaa ttcgaaaagg caaaagcggc cgcggtgctg atcgtgacgc
    16081 tcagggatat gtttcccgtc acgctcgggt tgcggctgtg gaccttgata tccttgatcg
    16141 cgtcggcgtc atcggttgtg gcattgatgc ggttttcacc attaaagatc ttctgcccgt
    16201 tgaactcggc gttgttgacc agtcgatcga tggcctcgat gtttgcatca atctgggcct
    16261 ggtacgcggc cttttcctcg gtggtcaccg tcgagccctg tgcggccacc acgagtccct
    16321 caatttcggc cgtcagtttg ccgatttcca tcagagtgct gtctgcgaca ttcaagaatg
    16381 actgattcct cgtgttaacg ctgatggcgg cgtcgacgga cgcaagttcg gaacgtaacg
    16441 agttcagggc gatgagcccg gccgggtcat cggcgccacg attgatgcgc cggcctgtcg
    16501 aaagccgggc catggtcacg gctcgctcgg actccaggcg gttcatggtg tggaggagtt
    16561 gatagagccc cgcgttggaa ccaatggtca tggacattgc ttcgtttccc catttaatgg
    16621 cgcgcaaagg gtagccctgg ccggggcgga ccgcttgagc gcgcgtcgcc gtccccgccg
    16681 tcgctgtctc acccttcccg gccggaggcg agcggacgtt ccgcccgtga cccgagccgg
    16741 ttttcgcgtg cacttgtccc gacgatcgac ttgccgactg tcggccggcc ggtcgaaacg
    16801 tcgccccaac cgtaagatat aattttgcgg tccggaatct ttttttgagg aaggggactc
    16861 tggtcgtcat atctcattgt tgggtaatgg gttatgactt cagaagaccg gggctgccag
    16921 aggtccgcat tctcgggttt ttgctaaagt cacttgactt gggaaacgag ccggttctga
    16981 tcgcccgtgg cgagcgggcg agctgggatg ggagaggggc gtgggcgatt gtcggcggtt
    17041 tttctaggtg gctggttggg atgcagccgg tgctccgctg ccggccccgg gtgttcctgt
    17101 taagggcttg tttttgagaa gagagtcgag cagggaacga gccgtgccac ccgacaccgc
    17161 tccgccgccg acgtagaccg cttcgacctt tccgttactg ccgacgacaa agaaggtggg
    17221 gtagcctcgg accaagaact gctcgccgat cttgtggtcg ggatccatcg tcacgggcat
    17281 ggagaggttc cattccttgt aggttgccag cgactgctct tgggttctgg ctccgcggcc
    17341 gcttcgggca tcctggttga tggcgatgac ttcgacgccc ttgtccttaa actctttgtg
    17401 caactgctcg acagcgggca tggcggcttt gcagaacccg caccaggagg cgtaaaaggc
    17461 caggagttgg accttgccgt tggcgctgcc gatcttgatt tccttgccgt caggtgtctg
    17521 ggccgacact gacggtgccg gcttgcctgt gagctgcatc gccgggtctc gctgttctgg
    17581 ttgggtggcg accggagcag caggctgttg cgggaaggcc tcgcggttct tgcctttcag
    17641 cagcagatcc agctcggttc gcacggtcga gtcgaaagtc ggggcggctc ccacatgagc
    17701 ggcctcaaca ataccgcttc ggccaacaag gacgaaggtg gggtaacctc tgaccttgaa
    17761 ctgccggccg atcgcctgcc tcggatccat ggtcatcggc atggacagat tccactcctt
    17821 gtatgtggtc agcgtttggt cctccgttct gcctcgggct ccttcgcggc tgtcctggtt
    17881 gatagcaatc acctcaatac ctttgttgcc gtaatccttg tggatctgct cgaccgtggg
    17941 catggctttc ttgcagaacc cgcaccagga cgcatagaag gccaacagtt ggatgcgttc
    18001 gcttccggct ccgatcttgg tttccttgcc gtcgatggtt ttcacggtcg ccgaaggagc
    18061 ggggttgccg acgagttcca tgaccgggtc gcggcgtgcc gccggctggg tggcggtcgg
    18121 ctgagttgcg gccggcggca gggcctcggc gacggccggc gggaagtcgg agcgagtctt
    18181 gccttgcagg agcaggtcga tctgcgtcgg gagcgtggaa ccgaagggca cggtgtgtcc
    18241 gaagtggact gcctcgacga ctccggactt gccgatgaga acgaatgtcg ggtggctcct
    18301 gacccggaac ctcggcccga tcaactggta tggatccata atcatgggca tggacaagtt
    18361 cagttctttg tagtgggcca agacctgttc ttcggtacgt ggctgcctgc ccgtgcgcac
    18421 gtccaggttg atggccagca cctcaacgcc cttatccttg tatcttttgt ggagatcttc
    18481 aacaccggga agggcttgcc tgcaatagcc gcaccacgag gtgtagaaag tcagcagcct
    18541 gaccctgcct gccgggccgc cgatctgaag gggccggccg tcaaaggtgg cggcattgat
    18601 gaccggcgcc ggtattccga tcaactgcat ggccggctgt gaatctgacg gtgtctccgc
    18661 agcctgtgga ctgagagtag cccgctgggc gtcggcgaaa acgtcggggg gggcgcccgt
    18721 cggccgcgtg gccgtcgcag ggctgcgaac ggggatctta atttccgctt gttccgtgtc
    18781 gtcggtcttc agcgtgatga acgtgccttc ggcgggcgcc acgtagttcg ccgggaacgt
    18841 cagattgacg cgatagtctc tgccgggctt gagggtgctg acattcactg caatgttcga
    18901 gtctgccgag acggcttcca gcagtctgac gtcgttgttg ctgttgtttg tgaaagcgat
    18961 gacgcgagtc ctcggccggc tatcgacagg gactaacagc atccgcggct cgatgtcgag
    19021 tcgttcgggc acaaccgcgg tgcatggtat ccttacggga ggaatgccct cgatgccgag
    19081 gtcgatctca atggtctgtt ggttgcgctt gccggggtaa ggggggttgg cggacaccag
    19141 aagctgatag gtcttgccgg gcgagatctc cttgagctcc cacgtgaaga cctgcatggc
    19201 ggggagcagc ccccgggtcg ccagtttgag ctccttgtcg gtcttgttcg tcaacatgac
    19261 ggttgtctgt gcgctggcgt caggccgcaa cttgtcgaaa agcacaaagg agggattggc
    19321 ctcaaggtgg tgctgcatat ggacggagag ggtgaggtgg acgatcttgt ttagcgggtc
    19381 gttcgtctcg acgtcgatcg tggtggagta gtctcctctg taccggtcgg cgttcaggcg
    19441 gaagaggaaa tcggccgagg agccgggggc aatggttttc gggtaccccg ccggggtcgt
    19501 gcagccgcac ttgggcttga cggccaggat tttcagttcg gcgtcaccga cgttgcggat
    19561 gttgaaggcg tgctccaact tgtcacccac ccagactttg ccgaagtcgt gtttcggctc
    19621 ggcgatctcg attcttggtg tgccgccgcc ggtcgttgcg ggcgggacga tgccgggtgg
    19681 ttcttgagcc ccaatcgcca gggcgcaggt caaaacccaa accgcgaggg ctgaaatgat
    19741 cgctgatacg caaagagact tcctcataat cgagcgtcct tcgtttgaga caagtccgta
    19801 gctgtggctg ccctgaacaa ggggatgctt gagggcgcgc ttccgcccat tatagcggca
    19861 ttttcggttc gggaaatgcg tccgtgaccg gttgtccgat aactggtagg attgtcgcct
    19921 gcagatggca aggcagctac cgggtcagga tccatgattc gaagcgaatc gagtcgcggt
    19981 ccattcgacg cagtcctgct tgtgtcgttt ggcggaccac aaggtcctca agacgttggt
    20041 ccttttttgg acaacatact tcgtggccgg cccaccccgc ctggccgcgt cgaggccatt
    20101 atcgccaact acatgcgata caacggggta tcgccgatca ccgaaatcac ccggcgtcag
    20161 gcggccgggg tgcgtcaacg tctggcagac gcgggtgttg agctgcccgt gtacgtgggc
    20221 atgcggaact ggcatcccta tctggcggat gtcatggccg agatgagccg ggcgggggtt
    20281 cgccgggcga tcggcttcat caccgccgcc catcattcgt atgccagttg cgggcagtac
    20341 aaggagaacg tgcgcgaggc ccgacgacgt cttgcggagc tgggtcttcc cgacgtcgaa
    20401 gtcacgtatg tggagagttg gtacgatcat cctggcttca tcggcacgct ggctcggcag
    20461 gttcgggctg cgattgacag gcttgaggcg ccgctgcggc ccaaggcaag gatcgttttc
    20521 acggctcaca gtattcccgt tgcgatggcc gagagttgcc gttaccgcga gcagttgcag
    20581 acaacggccc ggatggttgc cgaagatctt ggccgcagag actgggcgtt ggtctaccag
    20641 agccgcagcg gtcctccgca ggacccgtgg ctcggaccgg atatcaacga ttacctgaag
    20701 gccgagcacg aaaagggact tgaagcagtg gtggtctcgc cgatcgggtt catctgtgat
    20761 cacatcgagg tattgtgcga cctggacgtg aaggtcggcg acctgtgccg cgagatcggc
    20821 ctgcccatgg ctcgggccga gaccgtcaat gacgatccga tgttccttga catgatgacc
    20881 gaggtcgtgc tacgagcctg caaccgccac aagcgatttc caccactgat gatcacatcg
    20941 cgatagcagt gttcaccgca tgcaggcgct ctccacgcgt actcccagga aatgctttcc
    21001 acgccctgcc gaccgccggc tcggccatgt acgctccgga ggtcagagca atcagtgatg
    21061 agacgcacct caaggtgcgc gactgaggac gagaaagacg atgaccagca gagcgaatga
    21121 tgccatttgt catactcgcc gaattgacat tctccggcac gcagtgagcc tcaaaccgac
    21181 aacctcgtcg gtgatgcttc gcccatgcga gggccggtcc ggatcgtgtg aggataaccc
    21241 tggcaaggcc gcggggccag agcgtttcta tgcaacaaga atcccctctt tcgcggcaag
    21301 aaaggaatgg ttcgcgggat gcggaacaag cggtcgtcca ccggtcgcat tgaggacctg
    21361 cagtcttgct ctggcatgcc aggagagact gtaatagttg tatggttcac atgaccaaag
    21421 ctatctacgc agacggggtc ttcaagcctg aggagaagct cgatttgcct gatcagcagc
    21481 gggtgcaagt gattatccag ccaatagaag agcaggcaga caccaatcac accgcagccc
    21541 tggatcggtt cttcgaccac gtgtcccgtt cagagttctc ttatacgggc cccatgccca
    21601 cccgcgacga gctgcatgac cgcgtttgac acgaacgtct tgatatactc ctgtaacaag
    21661 gcggataagg tgcgccagcc gatcgcggcc cgcctgctcc gagaaaccgg accgatggca
    21721 ttctgctctg gcaggcggcc tgcgaattca tcgcagcttc ccggaagctg atcgtatacg
    21781 gtttcagcca ggctgaggca tggcagtatt tgcgcgagta cctcggtttc ctgaggctgg
    21841 ttctgccatc ggcgcagacg ctcgatcttg ctcagagact ccacattgaa caaggctggt
    21901 cattctgggg cgctatgctt gttggtgcgg ccctggatgc aggcgcgagg acgctctact
    21961 caaaagatgt ccccgggcgc agggtagagg gtattgagat catcgatcca ttcgccggtt
    22021 ctgtccatta gagcctcgca acggggatga ggaggcaatc gccctggcga aatgccatcc
    22081 cttgccaaac gagcccttcc ccgcgacagt agtgtcatgc ggaggactcg gcgggatttt
    22141 ctcgggaaga cgattcgcgg cgcggcttcg gtctcgctgg ggggctcgct gttgtcggcc
    22201 ggggggtgtt ttcggagcga accgccggac gggcgaatcc atcttcgcta ctgggacaag
    22261 tgggacggtt tcgaaggcga cgccatgcgg gcggtggtca aggacttcaa cgactcgcag
    22321 ggcggcatta cggttcacta tacgcccgtc ggggtgatcg accgtaagct gctggcggcc
    22381 acctcgggtg gcgaccctcc ggacgtggcg gggttgtggg agcacaatgt cacgcccttt
    22441 gccgaacagg gggcgttgac gccgctcgat gagctcatga gccgcgacgg gatcggccgc
    22501 gaccactgga tcgcggcgta cgtgaagatc tgcacgcata agggcatcat gtgggcggtg
    22561 ccgacgacgc cctttgccag cgggctgcac tacaacgcgg ccttattcga ggcgtccgct
    22621 gatcagcttc gggccagagg gctcgatccc ggtcgcgggc cggcgacgct ggcggagctc
    22681 gacgcctacg ccgaggccct gaccaagtac gatgcctcgg gcaatcttgt gcaattgggt
    22741 tttctgccac aggagccggg acgattcatc tgggcctggg gtttctggtt tggcggcaag
    22801 ctgatcggcg aggacgacac aatcaccgcc gatgacccgc tcaacgtggc ggcctatgag
    22861 tggatccaga gctactcccg caaatacggc gttgatcgga tcaagcgctt ctccagcggg
    22921 ttcggcaacc tggcgtcgcc gcagaatccc tttttctcag gtaaggtggc gatggtcctg
    22981 gacggtgcgt ggatgcacaa ctatatccag cagtacgctc ccggcatgcg ttacggcgtg
    23041 gtgccctggc ccaagacgcc acatggacct gaacgcttca gcaccgtggg ttgtgacatt
    23101 atggtcattc cgcgcggcgt gccggccgag cggcgcgaag ccgcctggca gttcgtcaag
    23161 tacgttatct cgcagccggc gatggagaag ctgaacctgg gacatcgaaa ggcgaccccg
    23221 ctgaccaagg tgagcgacga ctttctcaag aagcacccgc accgttatct cgatttgttc
    23281 atcagcctgt ccaagagcaa gggggtggtt tcgcctctgc acgtgggcat ctggagccag
    23341 tatcagacgg agcttttcgc cgcgttcgag cgggtgcggt tgctgcagag caatccggcg
    23401 accggcaagc cctacacagc cagggaggcg ctcgatatcg tccaggaacg gatgacccgg
    23461 gcatacgccc gatacaaaag gtcgctctcg ttgcgtccgc cgatctcggg gaggttgtca
    23521 tgacccgcgc agaccggcga catcttgtca acgggattct tttcgcctca ccgtggctga
    23581 tcggcctgag tgttttcctt gcgtatccga tcctttcgtc gctgtactac agtttttgcg
    23641 attactcgac gctgcgaccg gccgtctggg tggggttgca gaactatcag gatctcggtc
    23701 aggatgcggt gatgttcaag tcattgcgga acacgctggt tttcggggcc attgcgttgc
    23761 ccgggagcct gacggcggct ctgatgctgg ccctgctgct gaattcgggc gtgcgcgggt
    23821 tgccgatctt caggacgatc ttttacttgc cgtgcctcgt gccggccgtg gcttcagcca
    23881 tgttgtggtt gtgggtgctc aacggggatt tcggcattct caattatgcc ctgggagctc
    23941 tgcttgggcc gcttggtctg cggccgccca cttggctggc cgatccgctg tgggccaaac
    24001 cggcattggc gattatgagc atctggggct gcggtgggac gatggtgatc tatctggcgg
    24061 gtttgcagga cgtgcccgcc gaactgtacg aagcggcgga gatcgacggg gccggctggt
    24121 ggcggagact gtttcacgtc acattgcctt gcctgtcgcc ggtgatctat ttcacgctga
    24181 tcatcggaat catcgccgtc ctgcaggttt tcacgccggc gtacatcatg ggcggcggcc
    24241 cgcagggtgg aacccagggc cagccggcgc agtcgacctt gttctacgcc ctgtacatgt
    24301 gggcccaggc gttcgagtgg cttcggatgg gctatggttg cgcgatggca tggattctgt
    24361 ttgtcatcat cctggtgttg acgctcatcg cccaccggac ggccggcaag cgggtgacgt
    24421 atgttgggtg agtgctgatg gaacctcgga ctctgttcgg caaggtgctg gcgtacgcgc
    24481 tcctggtgct tgggagcttc ctgttcatct tcccgttcat ctggctgttg agcacgtcgc
    24541 tcaaaccgct tgagcaatcg acggccatgc cgccgcagtg gattccccgc cagtaccggg
    24601 caaccatcga cggccgcgag tgcgaagtgg ttcgcggcga gacgcttgcc gcgcggatgc
    24661 tcgtagttcg actggacatc gaggacgaga cggacccgcg gggagggcaa aagctgcttg
    24721 taccggaaag cgactatcag gacggcacga tcccgctcaa agtcgccgga atcgaacaac
    24781 ggataccagt gacggttctg cgcagagccg agccggggtg gtgttacatc aaggagcgca
    24841 agacgctggt ggacatgaac gccccggccg tgtgggacat cgtgccgccg gaagccgtcg
    24901 agagccgcgc gaagctattt tggggcaact ttccggccgc gatcaataag atgggtacgg
    24961 cgcgggtctg gatgccgttg ctgggcgagc gggaggtttc catcttctgg ctgtatttgc
    25021 gcaataccct gattgtgtgc cttctgggcg tgaccggtac ggtgtgcagt tcgtcgctgg
    25081 cggcttacag cctggccaag attccctggc gcggccgtgg cgtcctgttc ggtcttacgc
    25141 tggcaacgat gatggtgcct ttcgccgtgc tgatggtgcc gttgtacacg ctgttcgcca
    25201 gattcggctg gatcggcacg ttgttgccgc tgtgggtgcc ggctttcttt ggcagcgggt
    25261 tcaatatctt cctgctgcgg cagttctttc tgaccatccc aaaggacctt tccgaggcgg
    25321 cccggatcga cggatgcagc gagttcggca tcttctggcg ggtcattctg ccgctgagca
    25381 agccggccct ggcggtcgtc gctttgtttc atttcctgta cgcgtggaac gacttcatgg
    25441 ggccgctgct gttcctgact cgccgggaaa ccttcacgct ctcgctgggc ctccaggctt
    25501 tccagtccca gcacggcggc accgactgga ccagcctgat ggccgccagt acgctcatct
    25561 gcctgccgat catcgtgctc ttcttcttta cccagaagac cttcatcaag ggcatcgcga
    25621 cgacggggct gaaaggctga caagccacgg gcgtcgaacc tcggcgacaa caacggcaga
    25681 gtcgctccaa ggcggagcaa acgggatagc acgttaccgg cgcaccgatg tcgggtcgta
    25741 aggctccaag gcggtcttta gccgcaacca aaaccgggca ggcggattac gtttgcaggt
    25801 ctttggtact gaacgagttc tgcactcttg caaaggcatg cgcgcaaaat gcgcaccagt
    25861 cccgatggaa gaggctaagg cggaccgcct ttttgcgggc cgcaacccaa gcgctccgcg
    25921 aacaagaaac atgcgcgcaa aacgcacacc agtcccgatg gaagaggcta aagggccaga
    25981 aggagcgccg ccgctccctc acggtcgcgg ttctgaaacg gtcgccgctc gttcagaacc
    26041 ctgcgaagag accgcagccg ttccacgtcg gatctctcac ggacccggaa ccagtgtaac
    26101 ctgccgcaca tcctggggcg agagacaggg aattgcggcg cggactactg gtggccgaac
    26161 aattgcgttg ccgtcgccgc gatcgcgatc atggccagca tgatcgactc tccggcgatc
    26221 atcccggccg caacgggaaa cgtgtaggtc tccgcgcttt tcttgtgcgt cctggcccaa
    26281 gcccatgcga tcaccgcgcc gatcgcgaac gagaggctgt tctggaactg caccacccac
    26341 gccagaccca accccatcgc agagggcagg tacggggctg ctttgggcag ttttgccgaa
    26401 agtaccggca agatgatgcc gagcaccgca ccgatgagaa tcgcccattg ggcggtgatc
    26461 gggatggtat gcaggccctg ggtgagcgca tcagccattg ctttccacat gtacactgcc
    26521 ggcggattga acgcgtgcag tctctcggcg gtgggcacca tcagatacca cgcggggatg
    26581 atggcggcga cgccgaagaa gatgccggag aactgggcca ggaactgctt tcgcggattg
    26641 gcacccagca ggtaacccga cttcaggtcg gtgagcagat ctgcggcact gccggccgcg
    26701 gcggcggtgg taccggccga catcagattg acgacgtaat tgcccttcgc caggatcgcg
    26761 tagaccagct gagtcacctt tcccatggcc ccggtcgggg tggtgtcggt ctcaccggtt
    26821 gcccggcagg cgaccaacga caggacgaac gacatggcca ccgcaatgac gccgagcagg
    26881 atcgacatgt gaaacgcgaa gtagttgagg agaacaatgg cgacgccgat gggaatcagt
    26941 ccgccgatca accacgagct gggaacctct atcgcgtcta tggccttctg caagtcgacg
    27001 gacgacccgc gtgagtctcg gaccgtttgc ccggaatccg cgtgagttgt gcgacggaac
    27061 acgcgaaacg accgcgcgag ggtgcgccac tggaggccca cggacaccag gcttgagaaa
    27121 accatgatcg acgttccgcc ccacaaggcc catcgtgttg ggagaaggat cgctcccggc
    27181 ggcaagtccc cagccggcac gtaacccgaa gagcctgcga ctgccacttg agctccatgg
    27241 ctgacgagca acggcatcgt gacgtagtag agcagaattg aaccggcgag catcgagaac
    27301 gatacccgtg ttccgacgaa tgcgccggcg gcgatcatca gagtccccgg ctcgaagccc
    27361 aaacccgcca ggccggcctt cgcggcaccc tgtctgagcc agctcatcgg attgaagatt
    27421 ccgccgaaag gaagcagttc cggaatgtgc acgtatcgat agatcttgtc cagcgtggtg
    27481 atgacgccct cagggccgcg gaaaaaacct gtgacggcgc cacagcccaa cgcaatgatc
    27541 agggagtagg ccttcctgac ggcctcggcc ccgcgactgt gcaggctgcg aagtgtttcg
    27601 gcagcggcca tcccgctggg gaacggaagc tgctcgatat tgatcatttg ccgcttgagt
    27661 ggaatggcca tgaacacgcc cagggctgcg gtcagaaacg taaacgccac cagagtggtc
    27721 cagggcagat gtgtgccgct catcagcaac agggagccga cggccgtggc caaagtcccg
    27781 ccggtagaca tgccggcggc ggaggcggtg gactgcatgc agttgttttc gaggatggtc
    27841 atctcgctca atcgattgcc tgaaaccgcg cggaccgcgt tccagatcac gtaggacagg
    27901 acacacgccg taatcgccac gccgaaggac cagccgagtt tcagcgtcgt gtagaggttc
    27961 gagacgcaca tgaacattcc gaggattcct cccgtgagaa cggcgcggag cgtcaattgc
    28021 cggacgcgct cgccctggta gacgtgccgg taccagtgca gttctttctc ttcgggtgtg
    28081 gcgttttctt ggagcggcgg cacctcgcgg acgatccccg tcggggcacc ggcggcagag
    28141 gagccttttc tttcggccat gctccggcgg catccggggc cgtcgtcggg ccggccctgg
    28201 caacgattgc gatcatcggc tggcataaac gaccttcgaa cagaccatcg tcgattggcg
    28261 ggaaagttaa ccgtaaccgg tccaaagaac aaacattcgg accttaaggc aagcgagtct
    28321 agccgagggc ggagatgacc gatacaatag aattgtggtc aatgtcgtcg atcggatttc
    28381 cggcgacggc acctttcgtg ccttggtggc ccggatccgc gaattgagcc ccttggccgc
    28441 cggatcaggc gatgaaacac attcgccgag cggtgcgcag gggggggtgg tatccgtgga
    28501 cgggctgtgg ggatcgtttg cgcctgtttt ggccggcgcg gcggctctgg agctgaagtg
    28561 tcccctttta tacgttaccg cccatctgga cccggccgac gagatccgcg acgacctgga
    28621 attgtgttgt ggtcgcacgc ccgagctgct gagcgccttc gaagccgccc cgggccaggg
    28681 ggcggccagt gacgagattt acgctgagcg cattcggctg tgcgcccaat tgcagcgcgc
    28741 ttcccaaggc gaccaagagc ctccctcgtt catcgtcgcc ccgatccagg cactcatgca
    28801 atgcgttccc tcggaacggg ccttggccgg ccagttgctt actctctgcg tgggccagga
    28861 acatgacctg cagcgaatcg cacgctggct catggaccac ggctatgaac ggctggacca
    28921 ggttgagtct ccgggcgatt tcgcattgcg gggcgacatc ctggacatct ttcctcccgc
    28981 ccagaccgat ccgtaccgca ttgatttcta cggcaaccag atcgaatcga tccgcggctt
    29041 tgacgtgagc acgcagcgat ccgataccga attcgagacc atccagatcg cggccgcctc
    29101 ggtaagagga aggcaggacc attctccaca caagcaccag accgtcgagg ccagaggcgt
    29161 ggtttctcag aggagccatc ccg
//
LOCUS       DAOTPW010000035        28701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1709_length_28701_cov_5.272045, whole genome shotgun sequence.
ACCESSION   DAOTPW010000035 DAOTPW010000000
VERSION     DAOTPW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 28701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28701
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1709_length_28701_cov_5.272045"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="P5316_04905"
     CDS             <1..416
                     /locus_tag="P5316_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84329.1"
                     /translation="LGKLKDRKDLMAVLAEVYPKFKHFHRVQNSGQIWAFVHRMSPGD
                     WVCVPSKRKTIHVGEITGQYTFVADQWCPRLIVSAKWAWASAGTYMHPAEPVGRPLAH
                     GPLIQRRAGGGRRSDNPLAPPASLLVWRIFAKPLY"
     gene            463..1938
                     /locus_tag="P5316_04910"
     CDS             463..1938
                     /locus_tag="P5316_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84330.1"
                     /translation="MSLSRRRFLSQSASLSAFAVVGKAVAAKAAEQPAAMPPPKRGAN
                     ERIRVAVAGINGQGTSHMGGYAGMKDVEIVYLVDPDSRLFESRAKSVEDKAGYRPKCV
                     QDIRKVLEDKSVDVVSIATPNHWHSLMTIWACQAGKDVYVEKPMSHNIHEGRIAVETA
                     RKYNRIVQHGTQSRSDMKWAKVVAAIASGKLGKLLISRALCYKPGGGHNTRGDLGFKP
                     YTNPPKELDFDLWLGPARKQPYHENLVHYRWHWFWDFGNGDLGNQGVHQMDIARWGIP
                     GATLPKSVYSFGGRLGFFDQGEAACTQMAVMDFGETKLIFETRGLKSPKYMGVDVGNI
                     FHLEAGDIVNGKFYPKGSNEEAPLPEVEATRGPGGDRLVNFLAAVRSRKVSDLNADIL
                     EGHYSSALCHLCNMSIRLGSKIPFRPRDTTLESDPVVLDLLERTEQHLAENGVDIEKS
                     GFVLGRKLVVDAGTETIVDDPEANQLLTRHYRKGFEVPDKV"
     gene            2199..2684
                     /locus_tag="P5316_04915"
     CDS             2199..2684
                     /locus_tag="P5316_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84331.1"
                     /translation="MRPSYEVLREAADKIGVKALAATLRLSPALVYKWCQEYDENDPD
                     TSGARNPLDRLADIIDATGDVEVVNWLCNRAGGFFVPNPEMTVRDFSTDLLLGTQRLV
                     KEFSDMLEEVSRSVANDGAIQPREAERIRRHWETLKRVAEAFVVSGESGLFNPPSNSR
                     F"
     gene            complement(2701..3159)
                     /locus_tag="P5316_04920"
     CDS             complement(2701..3159)
                     /locus_tag="P5316_04920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRR84332.1"
                     /translation="MKTRFTSPDPGFRQRMRTSFSRQGFLRHLGVKMIDLQPGYCEME
                     VAFGEHLSQQHGYFHGGLVATVADVAAGYAAFSLTESDASMVTAEFKINFIAPAKGER
                     LIARGSVVKPGKTLTVCQCDVVCIKDEVETTCAVALATFIALPGKPEKTT"
     gene            3544..4815
                     /gene="glyA"
                     /locus_tag="P5316_04925"
     CDS             3544..4815
                     /gene="glyA"
                     /locus_tag="P5316_04925"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRR84333.1"
                     /translation="MATIASDFEAYPTIKNTDPAVYRALTAEGERQAFCLELIASENH
                     VSRAVLEAVGSILTNKYAEGYPGKRYYGGCKHVDEIEDLARERAKQLFHCDHVNVQPH
                     SGSQANMAAYMAVLEPGDTILSLNLSHGGHLSHGMKANFSGKFYNVVHYGVDPRTEVF
                     NFDEIRRLARECRPKMIVTGASAYPRTIDFAEFAEIAAEVGAYHMADIAHIAGMVAVG
                     LHPSPVPCADFVTTTTHKTLRGPRAGMVMCKAQHAKALDSRVFPGLQGGPLMHVIAGK
                     AVAFGEALKPEFKQYQERILANAQALAETLRSFGYRLVSGGTDNHLMCVDLRQVHADL
                     TGKQAETWLEMAGVIVNKNMIPFDERKPTETSGLRIGTPAVTTRGLNTAEMRIVGEFI
                     HKVLSSRGDEGAIASVRHQVREMCQQFPMPH"
     gene            4914..5486
                     /locus_tag="P5316_04930"
     CDS             4914..5486
                     /locus_tag="P5316_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84334.1"
                     /translation="MLPLMRADIHKREPLVGSIDPHEGCYWQSEKGTLNIVLARRGRS
                     LFDENFDFTWIMSLVLEDVPAGSERLYRATPQTMRLFQSHAGDHRRAKSTSGVVVIER
                     LAGDRLKGRFHLLVRQQQFTVLGGWGPPLYRAPMSIIVGEFVVARDPTRGRRLFEQTE
                     ADGFDRLADEIALTQPSVRVLVPATSSAPH"
     gene            complement(5502..6752)
                     /locus_tag="P5316_04935"
     CDS             complement(5502..6752)
                     /locus_tag="P5316_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRR84335.1"
                     /translation="MPILVHVDELQPGMRLKRAILRDKQVMLSCNTPLEDWQINSLRR
                     RFPDLTVQICDPILDELVEFQDDSHDHEVSATVNRQIGKLMHRVREILSSKTALEGSD
                     ISGLQRSVAEVMQYIMDNPAAAAVLTRFGTAGDYLQGHVGNVFYVSLLVGNTIRDYIF
                     RERQRTVRIETACVRYSMNLTPLALGCLFHDVGMLPIEHLYEKKGGLTQEERRLIRDH
                     PNRGVEMLPPKLDAVAKMVVRTHHENMDGSGYPQGVPVESLHVFSRVIRAADALDAAT
                     SRHIYKKARSAARVLWEMSSGPFKEHYDPPIVKILMALVQPFPIGAKVKLNCERYGVV
                     VRHNRKHPFRPTIIIAFDELGRKLKKRDLAPPIDLSKTNEVQIVEFEGDDLTCLSDGA
                     DGSVWGVPEITRDEKEDLFAYCYP"
     gene            complement(7186..8151)
                     /locus_tag="P5316_04940"
     CDS             complement(7186..8151)
                     /locus_tag="P5316_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRR84336.1"
                     /translation="MRFLEDHRVPKPVFFGLLMAVSLITTMLPTETLSRLRSLAQPLG
                     LPQWLMQTATLETHRTAMTLISRNVPVDQYQQLEIERDALLSEIATLRYQNTQLQATV
                     TELANVRGMGFPRQGTLIPARVLAMDAAPGRDTMLAGKGSLQKVEKGDWVVSSLTVGA
                     GVNEGVKPQSHVLGRQCLIGWVDQTNLLTSRVILLSDKLAGKSRPRRVLIHPADSSSR
                     PSEVQPEVFVLEPYGGGRMIIRDIPRRMVDAGQIQVGDLVTSAPDDTRLPLAVVIGPI
                     EDLQANRDKPICFDALVRHMIDPASLSEVIIVDSSQPDSLPIPSP"
     gene            complement(8379..9428)
                     /locus_tag="P5316_04945"
     CDS             complement(8379..9428)
                     /locus_tag="P5316_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRR84337.1"
                     /translation="MLNSLLGLFSMDMGIDLGTCNTLVCVRGQGVVLNEPSVVAVKKG
                     TNRVLKDGTAVGNVAKEMIGKTPGTITAIRPLMDGVIADFDITEAMLSYFIRKVHGRR
                     SFVMPRVVIAVPSGITAVEKRAVYNSAERAGARRVYLVPEPKAAAIGAGLPVAEPRAS
                     MIIDIGGGTTEVAILTLADISVCESIRVAGDDMDAAIVNYMKRTYNLMIGPQTAERIK
                     IEIGSVAETDQELTMEVRGRDMISGLPRKTFVSSEEVREALKEPVGLIVESVVRSLEK
                     AEPELAADLVEDGIWLAGGGALLRGLDQVINQATGLKVQICEDPLTCVARGTSIFLEN
                     LNEWKDTMESDEDDL"
     gene            complement(9706..9867)
                     /locus_tag="P5316_04950"
     CDS             complement(9706..9867)
                     /locus_tag="P5316_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84338.1"
                     /translation="MAQLSPTGPRVPPQNDLYTFHLIVAAALLLVGIIYVAVQTVSYF
                     GTFLPPSGG"
     gene            complement(9897..10787)
                     /locus_tag="P5316_04955"
     CDS             complement(9897..10787)
                     /locus_tag="P5316_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84339.1"
                     /translation="MTPVTKIFVVLVCLFAFIFTPLTIGFVAQSYHWKALATQYQENM
                     STMVATERAALASAASAVSHYQAQRENDRLQMQSLSNDISRLEQRIEQLINEKEACDA
                     SRASWESSAQTLSAQLKVINNHNQALIEENSRLTKSEQDLRARNLSLQDRVEELSANS
                     VTLAQQLRQKIEELTAVRQENDELRQSLQLGKAREALTSAAQPPAKAGIPTKVSEIRG
                     SVTEVRLNEGLASIDVGSTAGVAEGMVMAVVRDGDYICDLIITDKVDPTQSVGKIAVQ
                     GSKQIRPGDTVIDERSLMAR"
     gene            11513..12943
                     /locus_tag="P5316_04960"
     CDS             11513..12943
                     /locus_tag="P5316_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84340.1"
                     /translation="MSGQKLYRPGPGYWSWATVAVLALAARPSWSLAAAAIEGRLPDS
                     WLAAGVRQAWQAVVPVRHVDKIQAWHLVDAYLVAIGSDGSVRVFAADKGTHLWTQPMA
                     EPLSNLFPPTVYQDGDLRAMVFTIRDRAVFLDPATGSQFRRPHRLDNGDTELRPVGPV
                     HLWAGPISPVVANGDHVFQAAPRKRIRRYSISRDIQNYQVGIDADIVLPPLCIPEKDL
                     LVLADYNGTVAATEIDTRRTVFTAELNARPLGWVAADEQALYVVTSGPRLHVLDLADG
                     HERLEGYSRGFLLPAVPVEGPIVTSESVYVALEGDKLQRVGKELRWPNWTATGVRRFL
                     AEWPGRVALLAENGSIRFVRPETGETLTTVEPPVPGLDGLSNPLNDAIFLTSARGEVR
                     CLRPANAAPLKEADFRPVTTRPAGLPADKTAVVAAPDAAPTGEGKTTSEEASTGQETP
                     AASTENTGPKLSPIEALIADPLRSRR"
     gene            13187..14842
                     /gene="purH"
                     /locus_tag="P5316_04965"
     CDS             13187..14842
                     /gene="purH"
                     /locus_tag="P5316_04965"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRR84341.1"
                     /translation="MSNLKIKRAIIAVYDKTGIADFARALVDEFGIEIISTGGTAKHL
                     KEAGIPVTMVEEVTGFPEMLDGRVKTLHPMIHAAILADRDNPEHMKTLEKHGIKPIDM
                     VVVNLYPFEKTVADPNCTFEQAIEMIDIGGPCMLRAAAKNHKHVLVCCSPEMYDAYLL
                     LLRKWSDPALRGAALVEEACRVFRTTCLYDEAVSTWLSARINREFSQRTLRLRAWGKL
                     RYGENPHQQAEVLGNVVDSEEPALILASMPNESAEMSFNNYGDGDAALSLVKELSRAK
                     LKKHSVAFIKHMNACGCAIGDDPVDTYRRAYLGDPIAAAGGVLCMNFPVTLDVANTVM
                     NSFGSWGKAAGAAFFKLDVWIAPHFDEDAVKLITTPTEKRKWGKECRLLAVGAMDTPP
                     DPNELDYKKITGGMLVQTRDLLGLNEDQWKVVTRRKPTQSEMADLRFAWLVCKHTKSN
                     AITICKDDMLLGNGAGQMSRVKSCDLAIQLARENGHGDKLKGAVAAGDAFFPVPDGPQ
                     KLIDAGITAIIHPGGGNRDADTIALADRANVAMILTGTRHFKH"
     gene            14971..16449
                     /locus_tag="P5316_04970"
     CDS             14971..16449
                     /locus_tag="P5316_04970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HRR84342.1"
                     /translation="MSNPANFAAIDLGAESGRVILGTVSDSKLELSDLHRFPNGPVQT
                     LDELHWDVLRLWSEMKQGVGMAFQQASGLRGIGIDTWGVDFGLFARGNALLGNPFHYR
                     DARTNGILDKAFSIVPRKEIFDHTGIQFMQFNSIFQLLAMKLANSPLLDAAETFLMMP
                     DLFNFWFTGRKCCEFTNATTTQAYDPRAGAWAKALIEKLGIPTRIFPEIVPAGTVLGP
                     VCKVVRDEIGIDQVPVIAPATHDTGSAVAAVPAVGDAPWVFLSSGTWSLMGMEVTTPI
                     INDKSLAYNFTNEGGVGGTFRFLKNIMGLWLVQESRRTWQKAGHDYSYAQLTDMAGAA
                     RPFLAVVNPDDVSFLPPGDMPARIAQFCRKTGQAAPSTPGETVRVCLESLALTYRQTL
                     ERIEACTGRRAEVIHVVGGGTQNKQLCQWTADATGRTVVAGPIEATAAGNVAIQAVAT
                     GVLPDLAAARKLIRQSFNLITYQPKDTTLWDEVYQSFLKVRG"
     gene            16727..17821
                     /locus_tag="P5316_04975"
     CDS             16727..17821
                     /locus_tag="P5316_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRR84343.1"
                     /translation="MATENRLTVCGFGEVMVRLSPIGPQAFGDSNTIQITAGGSEANV
                     LAKLAGYSPGFRTELVTALNDDLAGRTLVADLRRYGVGLAHVRWTKNLRNGVYYLEQG
                     LGPIVARVDYDRKGSAIAELAPDESLFDVVQQSSAMFISGITPALSAHCAANTELALK
                     RAKLSGVPVFFDVNYRKKLWSPADAGKQIDTYLRTGWITALITTETDAKTVFGIDFGV
                     DDESPIDVLIERSRKVLETLKERYGNRCELYVLTIRKRVTNETGVWTSAALLPDGAYC
                     VGDVFDYVILDRPGAGDACSSGLIAGYLGIQKDGSFNRKADLASRVKLGLDLGNRAAI
                     VAQKTIGDMGPVWDVGMYFNRVSAGKEIAR"
     gene            complement(17917..18945)
                     /locus_tag="P5316_04980"
     CDS             complement(17917..18945)
                     /locus_tag="P5316_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRR84344.1"
                     /translation="MGSPYRRCRAYIHYLRKPILSFLPFLGLLVTVLIIGTFCFHFLY
                     THKRLSYAEAFYITYCLVWVEHLIEFPEHWLLRMFYFVLPPLGFLVFLDAVVRFSYHI
                     LRRDEAGEEWIRAMCKTLNNHVVLCGLGKLGLRTLQQLIRLGETVAVLEKNPHCPNLA
                     FARNNGIPVRLGHSREEGVFDDLNVAKAKSIILATNDDLANLEMAIDARKLNPSIRVI
                     IRVFDQELAARLRDSFDMPLAFSTSELAAPLFATSSSDPTIVNSFYVGDRLLAVANLT
                     VNEEAELVGRAIRDVGKHLHAFVLSHTRSGQTMLFPSADVVLEAGDAITVQAEPEDLR
                     AIHRMNQS"
     gene            19025..20053
                     /locus_tag="P5316_04985"
     CDS             19025..20053
                     /locus_tag="P5316_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84345.1"
                     /translation="MPDVADSYRDQTATVVGIDEAGYGPLLGPLVVSATAFDVPLSVL
                     KKVTEPAAGPDLWSLLADSVTRKASKREPRLAVADSKKLHGKGGGKHGLLLLEQAALA
                     FLAQVGPMPGSLRGLLRSFCPQVLGQLAGYPWYQGADVELPVDCGPDSVRLQAHSLHR
                     SLARAGIRFRGAWVEVLPEGHFNRQVETTRNKAVVLFGRTTRLIQRVIESLGPRPLGV
                     WIDRQGGRVGYRRPLMTAFQDARLEVLEESPDRSGYRLNRRPAPLVVRFVKQGEKHHL
                     AIALASIFSKYVRELFMICFNRYWCSQVAGLRPTAGYYEDGQRFLADIKDAEACRQVD
                     RVMLVRAL"
     gene            20129..20467
                     /locus_tag="P5316_04990"
     CDS             20129..20467
                     /locus_tag="P5316_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRR84346.1"
                     /translation="MPIENWSDNVLLVELADDPQFTDDLTTVLDTVEQRTDLDVVLNF
                     QEVTFLNSSNIARLLKLRKAVVINNKRKLHLCAISIHVWGVFLVTGLDKIFDVYDDVA
                     TGLAGLQLAQ"
     gene            20632..23463
                     /locus_tag="P5316_04995"
     CDS             20632..23463
                     /locus_tag="P5316_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84347.1"
                     /translation="MSISRRTVSIGLLALFAVAPLAPAADAPPEGEKAAVTVETDFVK
                     YTLGADARSLHFIDKQRGTDYCRPVPVAQVEIAGKRHPAGSAKADGDLVVLGFAGTDA
                     RAVLKLISHSRYFVVEVVSLEGPGVEVFTFFDLPLTLQAQADEPLAACTLALNLKTNV
                     REIPRPVSRLEALSYPRFGCAGAQAAVVACPPDQLRQILQQVVTDAPDLPHSPLGGPW
                     AMDHDVNKGSYLFDFGDLNEKTVDDWIRLAKRLGMNQIDFHGGRSFRFGDCRPNPEIY
                     PNGLASMKAVVDRLHEAGIKAGLHTYAFFIAKDAPWVTPVPDPRLGKDATYTLASDIT
                     ADSATVSVNESLEGRNLKTGFFERNSLTLQIDDELIVYAGLSEDKPYAFTKCQRGAHG
                     TKVAAHKAGAKVHHLKQCFFLFTPDGDSTLLEEVAQKTADTYNACGFDMMYLDALDGE
                     GILGGNEYGWHYGSKFVFELFKRLNKPPVMEMSTFHHHLWYVRSRMGAWDHPNRSHKR
                     FIDLHCEANAGLARMFLPGHLGWWAVKTYSDIQNEPTFSDDIEYLCGKCIGNDCGFSI
                     MGVNPGNINTVPVFGRLAEIMRNYEELRHAKYFDEATRTKLAEVGKEFTLFKAEDGRW
                     RLRPVQYAKHKVESREDCARIWRVENPYRRQPVRLRIEALPSISPYDTPGGLVVEDFS
                     DTSDFSERAVNTGVTADLRPSTGQVKVGAVSGAFTASNSGKVGRTAAWAKVGKKFAPP
                     LSLEKHEGLGLWVHGDGKGEVLNVQLTSPPHVSHGIGDHYINVDFTGWRYFELVEPEG
                     ERWRQYTWPYGWAYSIFREHVDFRQVETISLWYNNLPAEDTVTCYLSPIRGLSVFMNK
                     LKDPSITIGGKTLVFPVEMESGSYIEFNSPADCKHYGKDGGFIGDVNPQGDVPTLEAG
                     ANEVRFHCDTGGEAQPRARVTVISYGEPL"
     gene            complement(23441..24781)
                     /locus_tag="P5316_05000"
     CDS             complement(23441..24781)
                     /locus_tag="P5316_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR84348.1"
                     /translation="MSDAEQRVTLGVDQQKRRASVFRGNVLVLGLVSLFTDLSSEMMN
                     PLLPIFIAGLVPLGMAPVYVGLVEGVAETTASLLKLVSGRISDRLGRRKALVVAGYGI
                     STAVRPLMALAGLVGAAWGGWQVVGLKFLDRVGKGIRTSPRDALIGDSVGADVRGLAF
                     SFTRALDHAGAVLGSLVSVCILMAMLGYGIWRSSDAKPTQDEMTALRWLFGIALIPGL
                     LAVATLILKVREIAPRPRRSVSGAQESKGMATAAGAWKRLPRRFYPFVGVVVLFTLGN
                     SSDMFLLLYAWQKFRLGLPAVIGLWIVLHLSKIVFSFPGGMLSDRLGRRPMIVAGWVT
                     YALVYLGLSQAQAEWQFWALFLAYGFYYGMSEGAEKALVADFVPSQHLGTAYGVYNGA
                     VGLAALPGSLLFGVFWAVIGPGWAFGIGAALAGLAAVLLMVLLTETRITAARRS"
     gene            complement(24958..26238)
                     /locus_tag="P5316_05005"
     CDS             complement(24958..26238)
                     /locus_tag="P5316_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR84349.1"
                     /translation="MSDNAHSGLRDLSTAQWKAGIAAWLGWMFDGLDMHLYTLVALPF
                     VAELLRVSIKDPAAGYYGSWIQASFLVGWALGGAFFGRIGDRLGRTRTLMLTILTYAA
                     FTGLSFFAQTWWHLLIFRFLAALGIGGEWAVGASLLSETWPKRLRPWVAAVLQTSGNC
                     GVLLASLAYYVIARMEAPNRAVFLVGILPALIVFWIRRAVPESSHWEAVKRHERDQPG
                     IADLFRGSIRRTTLLTVFVCATTLTAHWAFLFWYLQHFHGLESLQGWSEARRNALVSQ
                     AMFLVMASAIAGNFLAGFLATRLGFRRVILLLCVAYFGSLTLAYGVPRDHRSLLACLP
                     LAGLSQGIFALFTMYLPPLFPTLLRTTGAGFCYNIGRVVAAGGTVFFGLFSKVGDHRP
                     AILCAGLLFLPAAVVAYFLPEDRWQRGHDAGPRA"
     gene            26284..26766
                     /locus_tag="P5316_05010"
     CDS             26284..26766
                     /locus_tag="P5316_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84350.1"
                     /translation="MGGRYHRADRFASCMYLIPSEPLIPHLRTDHKIEPEVFRARFPE
                     HPLCSERFAGFLAAHNVRKVNECLHYRLEVAGCPMTARYGVEHPLIPQPDPTFVWPLT
                     RREPRSGIGRAATGGPCPQWRVKGWTDPARDVAEAVEHNERVFLYGPSGTGKLRPTRN
                     "
     gene            complement(27065..>28701)
                     /locus_tag="P5316_05015"
     CDS             complement(27065..>28701)
                     /locus_tag="P5316_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84351.1"
                     /translation="EQNDRSEGFRQPRYDWDHDCDIDQVDFARWQYCLGRQEPQQSLW
                     DDYLCVELDSNDDRMVSIEDWDAFEANATGAGISYNPKPPPASSSPLDPCETPENYFT
                     ITAWRSVRTHGTVGPLGITLDPDAAPDSAVIEPRQGDVQIIEVDLAQPAYPWTDLSFV
                     YVTGSDASQPWPMSYDFVNNDQTFRLFFDATDPDNRLNDQVCYTIDLSDALISLNWTE
                     LECDADCSIRMLAADVDGDVTVEETDDEGAIEAANGQTAGESNMRLDIDTDGTITGTE
                     TTGADWTIVHALLDNTVVCSGGSMTMGISQSMSMAERGDQTQELTLLALLQQQLAAGL
                     LSPTANVSARWVVHGTGATSVTLPAGGGTVAVDLVVATDAPIWGLEGRLAVDAANVVS
                     INSADWTELDNVLFSHRVDGHNQSSYYDLTVMDWLWARMTPDGSDVIDSGTDVEALLA
                     PDPPTAWTLTNSLATVDGAIATPAGDLFTTTASIGGATRQLLPVGQTAVATLSLTISG
                     TPGTYHLTLNDGSFINEDMASVPMVGGPELEIIVGQ"
BASE COUNT         5589 a         9274 c         8459 g         5379 t
ORIGIN      
        1 acctcggcaa gctgaaagac cgcaaagacc tgatggccgt gctcgccgag gtctatccga
       61 agttcaagca tttccatcgc gtccagaact ccggccagat ttgggccttt gtccatcgca
      121 tgtcgccggg tgactgggta tgcgtgccca gtaagcgtaa gacgattcac gtcggcgaga
      181 tcaccgggca gtacaccttc gtggccgacc agtggtgtcc tcgtctgata gtctcggcca
      241 agtgggcatg ggcgtcggcg ggaacgtaca tgcatccggc ggaacctgtc ggtcgcccac
      301 tggcgcacgg cccgctcatc cagcggcgcg ccggcggcgg ccgcagatcg gacaatccgc
      361 ttgcaccccc ggcatcgctc ctggtttggc gtattttcgc caaaccgtta tactgacacc
      421 ctcaatcgtt cgtccagagt ccgtccaaca ggaggatcgc aaatgtctct gagtcgtcgc
      481 cgttttctgt cacagtctgc ctcattgagt gccttcgccg tggtcggcaa ggctgttgcc
      541 gccaaggccg ccgagcaacc tgcggccatg cctccgccca agcggggagc caacgagaga
      601 atccgtgtcg ccgtggcggg catcaatggg caaggcacct cgcatatggg cggctatgcg
      661 ggcatgaagg acgtcgagat cgtctatctc gtcgatccgg actcccgctt gttcgagagc
      721 cgcgccaaga gcgtcgagga caaggccggg tatcgaccca agtgcgtgca ggacatccgc
      781 aaggtcctgg aggacaagtc ggttgacgtt gtgtcgatcg caacccctaa tcactggcac
      841 tcgctcatga ccatctgggc ctgccaggcg ggcaaggatg tctatgtcga gaagccgatg
      901 agccacaaca ttcacgaggg tcgcatcgcc gtcgagaccg cccgcaagta caatcgtatc
      961 gtccagcacg gcacgcaaag caggtcggac atgaagtggg ccaaggtcgt cgccgcgatc
     1021 gcctccggca agctgggcaa actcttgatc tcacgggccc tctgctacaa gcccggcggt
     1081 ggtcacaaca cccgcggcga cctgggtttc aagccttata cgaacccgcc caaggagttg
     1141 gatttcgacc tttggctcgg gccggcccgc aaacagcctt accatgagaa ccttgtccac
     1201 taccgctggc actggttctg ggatttcggc aacggcgacc tgggcaacca aggcgtgcac
     1261 cagatggaca tcgcccgatg gggaatcccc ggagccacgc tgcccaagag cgtctacagc
     1321 ttcggcgggc gcctgggctt ctttgaccag ggcgaggcgg cctgcacgca gatggcggtc
     1381 atggatttcg gcgagaccaa gctcatcttc gaaacccgcg gcctcaagag ccccaagtac
     1441 atgggcgtcg atgtaggaaa cattttccat ctcgaagccg gcgacatcgt caacggcaag
     1501 ttctatccca agggcagcaa cgaagaggcc ccgctgccgg aagtcgaggc tacccgaggt
     1561 cccggtggcg atcggctggt caatttcctg gccgccgtca gaagccggaa agtctcggat
     1621 ctcaatgccg atattctcga aggccactac tccagtgccc tttgccatct gtgcaacatg
     1681 tcgatccggc tcggaagcaa gatcccgttc cgtccgagag atacgactct tgagagcgat
     1741 ccggtcgtcc tggatctgct cgaacgcacg gaacagcatc tggctgaaaa cggcgtcgat
     1801 atcgagaaga gcggtttcgt cctcggtcgc aagctggtcg tcgacgccgg gacggagacg
     1861 atcgttgacg acccggaggc gaaccaactg ctgacccggc attaccgcaa ggggttcgag
     1921 gtgcccgaca aggtctgaac accctcgccg gcctctatcg cttgccccgg ggcgcctgct
     1981 tgtccgagcg ggtgcctcgc tttttacggc aagggtgtgt tgtagcccct tctcgcgtat
     2041 gatgacggcc ccgaaaggag gaacgcatga ttctggccga gatgatcgct caaatcctca
     2101 gttacatact ccagcagatg gtcggcaccc tggtctcgaa cctgctcagc aattggtttg
     2161 ggacgggaat gtgatcggac tgccgcgtgg gagccgctat gcgaccttcg tacgaagtcc
     2221 tcagagaagc cgccgacaag atcggcgtga aagccctggc cgccacgctg aggctttccc
     2281 ctgccctggt ctacaaatgg tgccaggaat acgacgagaa cgatcctgac accagcgggg
     2341 cacgcaatcc gctggaccgc ctggccgaca tcatcgatgc caccggagat gtcgaagtcg
     2401 tcaactggct ctgtaacagg gccggtggtt tctttgtccc taatccggaa atgaccgtcc
     2461 gcgacttcag caccgacctg ctgctcggca cgcagcgact ggtcaaggaa ttcagcgaca
     2521 tgctcgagga agtcagccgg tcggtggcca acgacggggc gattcagccc agggaagccg
     2581 agcgaatccg tcggcattgg gaaacgctca agcgggtggc cgaggccttt gtcgtgtcgg
     2641 gtgagtccgg cctgttcaat ccgccgtcga acagccgttt ttgaactccc gactcgttgt
     2701 tcacgtcgtt ttctcgggct tgcccggcaa cgcgatgaac gtcgccaatg cgaccgcgca
     2761 cgtcgtctca acctcatcct tgatgcagac cacgtcgcat tgacagaccg tcagagtctt
     2821 gcccggcttg acgaccgaac cgcgggcgat cagccgctcg cccttggcgg gtgcaatgaa
     2881 attgatcttg aactcggcgg tcaccatcga ggcgtccgac tccgtcaggc tgaaggccgc
     2941 ataccccgcc gccacatccg ccaccgtggc caccaggccc ccatgaaagt agccgtgttg
     3001 ctgcgacaaa tgctcgccga aggcaacctc catctcgcaa tacccgggct gcaggtcgat
     3061 catcttcacg ccgagatgcc gcaaaaaccc ctggcgactg aagctggtcc gcatccgctg
     3121 ccggaaaccg ggatcaggac tcgtgaatcg ggttttcact cgatacctca tgctcccatc
     3181 tgcacgttac gggacttgag ccgtcgtgtt ctgatcgtct tgtatcgttc ctggatcccc
     3241 tgtcaaggct ttggcgagta atggaatacg agcccttgct cgcaccgcga cgcgccgccg
     3301 tcgtcggcct gctctccttc cgttcggccg gcgggcgcag cgcagccctc cgacggcaat
     3361 gtcgagaaag cccgttgctg ctcgggctgc tcgccggttc catcccggtt tcctccccgg
     3421 gccacaccgc cggccccggt ttgctttttt gccgcgtgca gactatttgt aggccctgaa
     3481 tggacccgcc ggtcgtttcc ggaccggttc cctccctgcg atttgaggta tggtatgaca
     3541 agcatggcca cgatagcgtc ggatttcgaa gcttacccga ccatcaagaa cacggatccc
     3601 gcggtttaca gagctctgac cgccgaaggc gaacggcagg cattctgtct tgaactgatc
     3661 gcttcggaga accacgtttc ccgcgccgtg ctcgaggcgg tcggctccat cctgaccaac
     3721 aagtacgccg agggttaccc tggaaagcga tactacggcg gctgcaagca cgtcgacgag
     3781 atcgaagatc tggcccgcga gcgcgccaag caactgttcc actgcgatca tgtcaacgtt
     3841 cagccgcatt ccggcagcca ggccaacatg gccgcctaca tggccgtcct cgaaccgggc
     3901 gacaccatct tgtcgctgaa cctgtctcac ggcggccact tgtcccacgg gatgaaggcc
     3961 aacttcagcg gcaagttcta caacgtggtc cactacggcg tcgatccccg gaccgaagtc
     4021 ttcaatttcg atgagatccg acggctggct cgggaatgcc ggcccaagat gatcgtcacc
     4081 ggggcctcag cctaccctcg gacgatcgac ttcgcggaat tcgcggagat tgccgccgag
     4141 gtcggcgcct accacatggc tgacattgcc catatcgccg gcatggtcgc cgtcggactc
     4201 cacccctcgc cggtgccctg cgctgatttc gtcaccacaa cgacccacaa aaccctgcgc
     4261 ggccccaggg ccggcatggt catgtgcaag gcccagcacg ccaaggccct cgactcgaga
     4321 gtgttccccg gcttgcaggg cggaccgctc atgcacgtga tcgcgggcaa agcggttgcc
     4381 ttcggtgagg ccctcaagcc cgagttcaag cagtaccagg agcggattct cgccaacgcc
     4441 caggccctcg cggaaaccct caggagcttc ggatatcgac tggtctccgg cggcaccgac
     4501 aatcacctga tgtgcgtgga tttgcgacag gtgcatgccg acctgaccgg aaaacaggcc
     4561 gaaacgtggc tcgaaatggc cggcgtcatc gtgaacaaga acatgatccc cttcgacgaa
     4621 cgcaagccga cggaaacgtc cggccttcgc atcggcacgc ccgccgtgac cacccgcggc
     4681 ctcaacaccg ccgagatgcg aatcgtcggc gagttcatcc acaaggtact ctcgagccgc
     4741 ggcgacgagg gcgcgatcgc aagtgttcgg caccaggtgc gagaaatgtg tcagcaattc
     4801 ccgatgccgc attgatgggt attcctgttc cagcccgatc cgtcttgctg tcggtctcgg
     4861 ccttggcttg gaccgcgacg tgtcttcccg gctgcgcggt gggaaaggtt cagatgctgc
     4921 ccctgatgcg cgccgatatc cacaagcgag aaccgctggt cggctcgatc gatccccatg
     4981 agggctgcta ctggcagagc gagaaaggca cgctcaacat cgtgctcgct cgccggggac
     5041 gttccctctt cgacgaaaac ttcgatttca cctggatcat gtcgctcgtg ctggaggacg
     5101 tgccggcggg aagcgaaagg ctctatcgcg ccacgccgca gaccatgcgg ctcttccagt
     5161 cacacgcggg cgaccaccgc cgggcaaagt cgacctccgg cgtcgtggtg atcgagcgtc
     5221 tcgccggcga ccgtctcaag gggcgattcc atcttcttgt gcgacaacag cagttcacgg
     5281 tcctgggcgg ctggggcccg cccttgtacc gcgcgccgat gtcgatcatc gttggcgagt
     5341 tcgtggtcgc tcgcgacccg acccggggca ggcggctctt tgaacagacc gaggccgacg
     5401 gcttcgatcg ccttgccgat gagatcgcct tgacgcagcc ctccgtgcgc gtccttgtgc
     5461 cggccacctc aagcgctccc cattgaccgt cacgaatcac cctacgggta gcagtacgcg
     5521 aaaaggtcct ccttttcgtc ccgcgtgatt tcgggaacgc cccagacgct gccatcggcc
     5581 ccgtcgctca ggcaggtcag gtcgtccccc tcgaactcga cgatctgcac ctcgttcgtc
     5641 ttggaaaggt cgatcggagg agcgaggtcc cgcttcttca gcttacggcc gagttcgtcg
     5701 aacgcgatga tgatcgtcgg acggaaaggg tgcttgcggt tgtggcgcac cacgaccccg
     5761 taccgctcgc aattcagctt gaccttcgcc ccgatcggaa acggctgcac caaagccatc
     5821 aggatcttca cgatcggggg gtcataatgc tccttgaatg gcccgctcga catttcccag
     5881 agcacgcggg cggcgcttct ggccttcttg taaatgtgcc ggctcgtcgc cgcgtccagc
     5941 gcgtccgcgg cccgaatcac gcgggaaaac acgtgcaggc tctcgaccgg aacgccctga
     6001 ggatatccgc tcccatccat gttctcatgg tgcgtccgga ccaccatctt ggcgacggcg
     6061 tcgagcttcg gcggaagcat ctcgaccccc ctgttggggt ggtcgcggat cagcctgcgc
     6121 tcctcctgcg taagcccccc ctttttctca tacaagtgct cgatcggcag catgccgacg
     6181 tcgtggaaca gacagcccag cgccaatggc gtgagattca tgctgtagcg gacgcaggcc
     6241 gtctcgatgc ggacggttcg ctgccgctct cgaaagatgt agtctcgaat cgtgttgccc
     6301 accagcaggc tgacgtaaaa aacgttgccc acgtgcccct gcagatagtc gccggcggtg
     6361 ccgaatcggg tcaggaccgc ggccgccgcc ggattatcca tgatgtactg catgacttcg
     6421 gccacggaac gctgcaagcc ggagatgtcc gagccctcca gtgccgtctt gctgctgagg
     6481 atctcgcgga ctcgatgcat cagcttgccg atctggcggt tcaccgtggc cgatacctcg
     6541 tggtcgtgcg aatcgtcctg aaactcgacc agttcgtcga gaatgggatc gcaaatctga
     6601 acggtcagat cgggaaagcg tcggcgcagt gagttgatct gccaatcctc aagcggggtg
     6661 ttgcaggaca gcatgacctg cttgtcgcgc aggatggctc tcttcaggcg catgcccggc
     6721 tggagctcat cgacgtgaac gagaatcggc ataaggcctc tatcccgatt cccctcccgc
     6781 atacgtttcc gataaaccgg tgctcggctt ccctggagtc gagcaatcgg ccggcgcttg
     6841 catgcctgcg tcggacccgg gatcgcgccg caacccgacc cggcatgagt actcttctcg
     6901 cttgccgact cccgccgatt cggcggcgcc tctggcggct tgcccgctgg tacacaacaa
     6961 accgactcga aacggcacgg ctgtcgccga ccgccatgcg ggcgcagtac tcccgtgccc
     7021 ttgtttgccg tgtctggatt cgaccggcga tgcaccatac ttgagtactc gccggttgct
     7081 cgaaccgacg gcgctcacaa gacccgcgac tcgacgtccc gaatcggcgt ccaataacca
     7141 accccctgat cccgacggct tccgcaagta ccctcgcagc ctcggttaag gggagggtat
     7201 gggcaaggaa tccggctggg aactgtccac gatgatgact tcactgagac ttgccgggtc
     7261 gatcatgtgt cgcaccaggg cgtcgaaaca gatcggcttg tcccggttgg cttgaagatc
     7321 ctcgatgggc ccgataacca cggcgagcgg caggcgggtg tcgtccggtg cggaggtgac
     7381 aaggtcgccg acctgaatct gaccggcatc caccatccgg cggggaatat cgcgaatgat
     7441 catgcgcccg ccgccgtacg gctccagaac gaacacctcc ggctgaacct ccgaaggacg
     7501 actggaagaa tctgccggat ggatcagcac ccttcttggt cgcgacttgc cggccagctt
     7561 gtcgctgagc agaatgaccc gcgaagtcag caggtttgtc tggtctaccc agccgatcag
     7621 gcactggcgg ccgagcacgt gagattgggg cttcacgcct tcattgaccc cggctccgac
     7681 cgtcaggctt gagaccaccc agtcgccctt ctcgaccttc tgcagactgc ctttgcccgc
     7741 cagcatcgtg tcgcgacccg gggcggcatc catggcaagc acgcgagcag gaataagcgt
     7801 tccctgacgg ggaaacccca taccgcgcac gttggccagc tccgtcacgg tcgcctgcag
     7861 ttgagtattc tgatagcgga gcgtcgcgat ttcactcagc aaagcatctc gctcgatctc
     7921 caactgctgg tattggtcga caggaacgtt ccgactgatc agcgtcatgg ccgtccgatg
     7981 cgtttcgagt gtggccgtct gcatcagcca ttgggggagt cccagcggtt gagccagact
     8041 ccgaagccgc gagagcgtct ccgtcgggag catggtcgtg atcaacgaga cggccatgag
     8101 cagcccgaag aaaacaggct ttggaacgcg gtggtcttcc aagaatcgca tcgcagctcg
     8161 cgaccggcgg cggccggttc ccgcagtccc catggaacaa tggccgcggt gcggggtcgc
     8221 gccgggccat cgatgatctg aacacctgac tctctgtccc aaaacttcga gaacaaccgc
     8281 catcttgacg ggctgtcgaa ggggccaaaa cccgttctac gctgtcccgt ccccggttgt
     8341 ttcaggatgt actcaaagcc ggagggatac gtccgcgatc agagatcgtc ctcatcggac
     8401 tccatcgtat ccttccattc gttcagattc tcaaggaaga tgctcgtccc gcgagccacg
     8461 cacgtcagcg ggtcctcgca tatctgcacc ttcaagccgg tggcttggtt gattacttga
     8521 tccaaccctc gcagcagggc cccgcctccc gccagccaga tcccgtcttc cacaaggtcc
     8581 gccgccagtt cgggttcggc tttctccaat gaccgcacga ccgattcgac gatcaacccc
     8641 accggctcct tcaaggcctc tcggacttcc tcgctgctga caaaggtctt gcgaggcaaa
     8701 ccgctgatca tgtcacgccc gcgaacttcc atcgtcagtt cctggtcggt ctcggccacc
     8761 gagccgatct cgatcttgat tcgctcggcg gtttgcggcc cgatcatcag gttgtaagtg
     8821 cgcttcatgt agttcacaat cgccgcatcc atgtcgtcgc cggccacacg gatcgactcg
     8881 cacacgctga tgtccgccag cgtcagaatc gccacttccg tcgtgccgcc gccgatgtcg
     8941 atgatcatcg atgctcgcgg ctcggcaacc ggaagcccgg caccgatcgc ggcggccttg
     9001 ggctccggaa ccaggtacac gcgccgggca cccgcccgct ccgctgagtt gtagaccgct
     9061 cgtttctcca ccgccgtgat gcccgaaggt acggcgatca ccacccgagg catgacaaag
     9121 gatctgcgac catgaacctt ccgaatgaaa tagctgagca tcgcctccgt gatgtcgaaa
     9181 tccgcgatga cgccgtccat caacggccgg atcgccgtga tggtccccgg cgtcttgccg
     9241 atcatttcct tcgccacgtt cccgacggcc gttccgtcct tgagcaccct gttggtgcct
     9301 ttcttgaccg ccactaccga tggctcattc aagaccacgc cctggccgcg gacgcacacc
     9361 agcgtgttgc aggtacccag atcgataccc atatccatac tgaacaagcc gagaagtgag
     9421 tttagcacag ccaagtcatc cttttcgtct tggtttgccg gcctggaaca gtgaaggtgc
     9481 ggaattcccg ctcgccccct tgcacgcccc cgccggcgtc ccagaggctt catcggttcg
     9541 ccaggtattg taaccgtaga tacttttctt gcgaatgctc atcttttcgg acgcccttac
     9601 ccatcccgaa ttccgggttg gcagccccct tatacaggtg gcgactttcc gtctgcccgc
     9661 gccgattcgc ccaccaagaa taaacctaga gactgaggcg gtccgttagc cgcctgacgg
     9721 gggaaggaag gtgccgaaat agctcaccgt ctggaccgct acatagatga ttccaaccag
     9781 caacaacgcc gccgccacga tcagatgaaa cgtgtacaga tcgttctgcg gcgggactct
     9841 cggacctgtg ggactcagtt gggccatagc ggtttcccct gtgccgagcc ggttgctcag
     9901 cgggccatca ggctccgttc gtcaatcacc gtgtcgccag gacgaatctg cttgctgccc
     9961 tgcacggcga tcttgcctac tgattgcgtc ggatcgacct tgtccgtaat gatcaagtcg
    10021 cagatgtagt cgccgtcacg aacgacggcc ataaccatcc cttcggcgac tcccgccgtg
    10081 ctgccaacgt cgatgctggc caagccttcg ttcagtcgaa cctccgtgac cgaaccgcgg
    10141 atttcactga ccttcgtcgg aatccctgcc ttggccggtg gctgagcggc tgacgtcagc
    10201 gcctcacgag ccttgcccaa ttggaggctt tgacggagct catcgttctc ctgcctcacc
    10261 gccgtcagtt cctcgatctt ctgacgcaac tgctgggcca gcgtgacact gttcgcgctc
    10321 agctcctcga cccgatcctg cagactgaga ttccgggccc ggagatcctg ttcgctcttg
    10381 gtcaggcggc tgttctcctc aatgagagcc tgattgtggt tattaatgac cttcagttgg
    10441 gcagaaagcg tctgggccga gctctcccaa ctggctcggc tggcgtcgca cgcttccttc
    10501 tcgttaataa gctgctcgat tctctgttcc agccttgaaa tgtcgttcga gagcgactgc
    10561 atctgcagcc ggtcattttc acgctgggcc tggtaatggc tgaccgcgct ggcggccgag
    10621 gccaacgccg cccgctccgt tgctaccatc gtggacatgt tctcctggta ttgcgttgcg
    10681 agcgccttcc agtggtagct ctgggcgaca aaaccgatgg tcagcggtgt gaagatgaag
    10741 gcaaataaac acaccagaac gacaaatatc ttggttacag gggtcaaagg ccgggctcct
    10801 accttcgcgt tgatggttcc accaggcctc tcacgcccga ggccaccggt agagagggat
    10861 cgcgccgcat cccgcgtcgt tgccggccgc cgggccggaa cgcttgatcc caggcgagcc
    10921 gacggcatct gccgccagcg ttttgaccgg actgtgtgtg ccggctccgc cagccggccc
    10981 ggtcggagtg atccttacct cctgatccac tacaacccat agcatatctg cgtggaaaga
    11041 gtttgcaagg cccataggcc cccatctcag atagctccat gacggccggg gcggggcacc
    11101 cttcctgcct tgtctcctcg tgtgttcggt tccccaggcc tccacaggcg gacacccctc
    11161 cgtgacgccc accgcatagc ccagccgaat gcgttcccga cccacgcagg ttgaccgtcc
    11221 catgctcctg gccgggccgg gtgggccgag gcttcccgct ctgggtgata cggcggccgc
    11281 tccgcgttcc gggctggcag gaccgcatcg gaaaggcctc aggacactgg ctcggagccg
    11341 gcacgccggc gtccccccca ggccttctcc ccctcgaacc ggcgccagcc cccccacgcc
    11401 gcgaccaact gaaccgtacg tcgggccgtt ttgctcttga ccgctctctc ggctcacggt
    11461 tgaataccat cggggccgac gaggaccttc cacggattca caggagccat acatgagcgg
    11521 tcagaagctt taccggccgg ggccgggcta ttggtcttgg gcaacggtgg cggtcttggc
    11581 cttggctgca cgaccgtctt ggagcttggc tgccgcggcc atcgagggcc ggctgccgga
    11641 ttcatggctg gccgccgggg tccggcaggc ttggcaggca gtcgtcccag tgaggcacgt
    11701 cgacaagatc caggcttggc atctggtcga tgcctatctt gtcgcaatcg gttccgacgg
    11761 ctccgttcgg gtctttgccg cagacaaggg aactcacctc tggacccaac cgatggcaga
    11821 gcccctgtcc aacctttttc ctccgaccgt ttatcaggat ggcgacctgc gcgcgatggt
    11881 ctttacgatc cgtgatcggg cggtcttcct tgacccggcc accggctcgc aatttcgccg
    11941 gccccaccgc cttgacaatg gcgacaccga actccggccg gtcgggccgg tccacctgtg
    12001 ggccgggccg atcagccctg tggtggcaaa cggcgaccac gttttccagg ccgctccgcg
    12061 aaagagaatt cgccgctaca gcatctcgcg tgacatccag aattaccagg taggcatcga
    12121 cgcggatatc gtcctgcccc ccttgtgtat tcctgagaaa gacctgttgg tgctggcgga
    12181 ttacaacggg acggtagcgg ccaccgaaat cgacacccgg agaaccgttt ttactgcgga
    12241 actgaatgcc cgccccctcg gatgggtggc ggccgacgaa caagcccttt atgtcgtcac
    12301 cagcggtccc cggttgcacg ttctggatct ggccgacggc catgaacgat tggaaggcta
    12361 ctcgcgcggg ttcctccttc ctgcagtgcc cgtggaagga cccatcgtca cgagtgaaag
    12421 cgtctacgtc gccctcgagg gagacaagct ccagcgcgtc ggcaaggaac tcagatggcc
    12481 gaactggacg gccacgggtg tccggcgatt cctggccgag tggcccggcc gggtggctct
    12541 gctggccgaa aacggcagca tacgctttgt tcgtcccgag acgggcgaga cgttgaccac
    12601 cgtcgagcct ccagttcccg gcctcgacgg cctgagtaat ccgctcaacg atgcgatctt
    12661 tctgacgtcc gcgcgtgggg aggttcgctg cctgaggccg gcgaacgcgg cccctctcaa
    12721 agaggccgat ttccgccccg tcaccacccg gccggcaggt ctgcccgccg ataagacagc
    12781 ggtggtcgcg gctcctgatg ccgccccgac cggcgagggc aagaccacct ccgaagaggc
    12841 ctccaccggc caagagaccc ccgcagcttc gacagagaac accggcccaa agctctcgcc
    12901 catcgaggcc ctgattgccg atccgctgag gtcgagaaga taagcggtcg gcggtcagca
    12961 ctcagctatc agctcccgtg ctgccaacgc cctcgtcggc gcggcttctc gtgctcatcc
    13021 ttccgccttc gtccctcact ctgcctttgt ggtccgacga gggcatcggc cttgtgaacc
    13081 ctcaaccctg cttgccacgg cgcagctcat cgagcgaagc cgggagccct tccccgtccc
    13141 cctcaacggt tttcaccctc ggcctcgaac gctatcatac gcccccatga gtaacctgaa
    13201 gatcaaacgc gccattatcg cggtttatga caaaaccggg atcgcggact ttgcccgggc
    13261 cctggtcgac gagttcggca tcgagatcat cagcaccggc ggcaccgcca aacacctcaa
    13321 ggaggcgggc atcccagtga ccatggtcga agaggtcacc ggcttccccg aaatgctcga
    13381 cggccgggtc aagaccctcc accccatgat ccacgccgcc atcttggctg accgcgacaa
    13441 ccccgagcat atgaagaccc tcgaaaagca cgggatcaag ccgatcgata tggtggtggt
    13501 caatctctac cccttcgaaa agactgttgc cgaccccaac tgcaccttcg agcaggccat
    13561 cgaaatgatc gacatcggcg gcccgtgcat gctgcgagcg gcagcgaaga atcacaagca
    13621 cgtactggtc tgctgctccc cggaaatgta cgacgcttac ctgcttctct tgaggaagtg
    13681 gagcgacccg gcgctacgcg gcgcggcact tgtggaagag gcctgcaggg tctttcgaac
    13741 cacttgtctt tacgatgagg cggtttcaac ctggttgagc gccaggatca atcgcgagtt
    13801 ttctcagagg accctacgtc ttcgcgcatg gggaaaactg cggtatggcg agaatcccca
    13861 tcaacaggcg gaagtcctcg ggaacgtggt ggacagcgaa gagccagccc tgattctggc
    13921 aagcatgccc aacgaatcgg cggagatgtc attcaataac tacggcgatg gggacgcagc
    13981 cttatcgctg gtgaaggagt tatcgcgagc gaagctgaag aagcattcgg tggctttcat
    14041 caagcatatg aacgcttgcg ggtgtgcgat cggcgacgat ccggttgaca cgtatcggcg
    14101 ggcgtacctg ggtgatccga tcgcggctgc cggcggcgtg ctgtgcatga acttcccggt
    14161 caccttggac gttgccaata cggtgatgaa ctcattcggc agttggggca aagcagccgg
    14221 ggcggcgttc ttcaagctgg atgtgtggat cgccccgcac tttgatgagg acgcggtcaa
    14281 actcatcact acgccgacgg aaaagcgaaa atggggcaaa gagtgccgtc ttctggctgt
    14341 cggggcaatg gatacgccgc cggatccgaa cgaactcgac tacaagaaaa tcaccggcgg
    14401 catgctcgtg cagacccgcg atctcctggg cctcaatgag gaccagtgga aggtcgtgac
    14461 caggcgcaag ccgacgcaat ccgaaatggc ggacttgcga ttcgcctggc tggtctgcaa
    14521 gcacaccaag agcaacgcga tcacgatctg caaggatgac atgctcctgg gcaacggcgc
    14581 cggtcagatg agccgggtga aaagctgcga cttggcgatt caactggccc gcgagaacgg
    14641 ccacggcgac aagctcaagg gggcggtggc ggccggcgat gcgttcttcc cggtgcccga
    14701 cggcccgcag aagctcatcg acgccggcat caccgcgatc atccaccccg gcggcggcaa
    14761 ccgcgacgcc gacacgatcg cgctggcgga ccgggcgaat gtcgcgatga ttctgaccgg
    14821 cacgcggcat ttcaagcatt gatatgaagc tgtcagcgat cagcggtcag ctgtcagctt
    14881 ggacggatgg caggatgaat gctaacggtt tggaggtctg gctgatagct gaccgctgat
    14941 agctttcgcc agatgaagaa agcgtgagat atgagcaatc cagcgaattt tgcggcgatc
    15001 gatctgggtg ccgagagcgg acgcgtgatt ctcggcacgg tatccgacag caagcttgaa
    15061 ctgtccgatt tgcaccgctt cccgaacggc cccgtgcaaa cccttgatga gttgcactgg
    15121 gacgtcttgc ggttgtggtc ggagatgaag cagggcgtgg gcatggcctt tcagcaggcc
    15181 tccggcttgc gcggcatcgg catcgacaca tggggtgtcg acttcggcct cttcgcccgc
    15241 ggcaacgcgc tgctgggaaa cccgttccac taccgcgatg cccgcaccaa tggcattctg
    15301 gacaaggcct tcagcatcgt gcctcgcaag gaaatcttcg accataccgg tatccagttc
    15361 atgcagttca attcgatctt ccagctcctg gcgatgaagc tggcaaactc gccgctgctc
    15421 gacgcggccg agaccttcct catgatgccc gatttgttca acttctggtt caccggtcgc
    15481 aaatgttgcg agtttaccaa tgccaccacc acccaggcct atgacccgcg cgccggcgcc
    15541 tgggccaaag cgcttatcga gaagctgggg atcccgacga ggatcttccc cgagatcgtg
    15601 cccgccggta ccgtcttggg ccccgtctgc aaggttgtcc gcgacgagat cggcatcgac
    15661 caggtaccgg tgatcgcccc cgccacccat gacaccggtt cggcggtcgc cgccgttcct
    15721 gctgtcggcg atgccccctg ggtattcctg agttcgggca cgtggtccct gatgggcatg
    15781 gaggtcacga cgccgatcat caatgacaag tctcttgcct acaacttcac caacgaaggc
    15841 ggcgtaggcg gaaccttccg gttcctcaag aacatcatgg gactgtggct ggtgcaggag
    15901 tcgagacgta catggcagaa agccgggcac gactacagct atgctcagtt gaccgacatg
    15961 gccggtgccg cccgaccctt cctcgccgtg gttaatccgg acgacgtctc cttccttccc
    16021 cccggagaca tgcccgctcg cattgcccag ttctgccgca agaccggtca ggccgctcct
    16081 tccacccccg gcgagacagt ccgcgtttgc ctggaaagcc tggctctaac ctatagacaa
    16141 actctggagc gaatcgaggc ctgtacgggc agacgggccg aggtcattca cgtggtcggg
    16201 ggaggcaccc agaacaagca gctctgccag tggaccgccg atgcgacagg tcgcaccgtt
    16261 gtggccggcc cgatcgaagc cacggccgcc gggaatgttg ccatccaagc cgtcgccacc
    16321 ggggtgttgc ccgatttagc ggccgcccga aagctcatcc gccagtcctt caacttgata
    16381 acgtatcagc ccaaagatac caccctttgg gatgaggttt accaaagttt cttaaaagtt
    16441 agggggtaaa gccaggagca atcgcaggca gaaaaaaact gaggtcggac gcccgggttt
    16501 gcgcaataat gtggccccgg cgtggcaaac cggggccagg gaaggacttg acgcttccgc
    16561 aagtcgcgga gggagagagg agagcacgcg gaagcgtaac cccagaagga gcatacccca
    16621 tcggcgcctc atggtcaaga ggactaagcg attctcttaa acaatttttt catgccaccg
    16681 gtaatgcgat ttaccgacgc tcctatgcgg tggtggagga ttctcaatgg caacggaaaa
    16741 ccgtctgact gtatgcggct tcggggaagt aatggtgcgg ttgtctccaa ttggccctca
    16801 ggcctttgga gattccaata ccatccagat caccgctggg ggatcggagg ccaatgtatt
    16861 ggccaagttg gccggttact cacccggatt caggacggag ctggtgaccg ccctgaacga
    16921 tgacttggcc ggccgcacgc tggtagccga tttgagacga tatggggtgg gcttggccca
    16981 tgtccgttgg acgaagaacc tccgtaacgg cgtctattac cttgaacagg gactcggccc
    17041 gatcgttgcc agggtggatt atgaccgcaa gggttcagcg atcgctgagc tggccccgga
    17101 tgaatcatta ttcgacgtcg tgcaacagag ctcggccatg ttcatcagcg gtatcacccc
    17161 cgcgctgagc gcccattgcg cggccaacac cgaactggcc ctcaaacgag caaagctgtc
    17221 cggcgtgccg gtctttttcg acgtgaacta ccgaaagaag ctctggtcgc ccgccgacgc
    17281 cggcaagcag atcgacacct acctgcgcac cggctggatc accgccctca tcacgacgga
    17341 aacggacgcc aaaacggtct tcgggatcga cttcggcgtc gacgacgaat cgccgatcga
    17401 tgtgcttatc gaacgcagcc gcaaagtgct cgagaccttg aaggaacgct acggcaacag
    17461 gtgcgagctg tatgtcctga ccattcgtaa gcgggtgacc aatgagacgg gcgtctggac
    17521 ctcggcggcg ctcttgcccg atggcgccta ctgcgtgggc gacgtgttcg actatgtgat
    17581 tctggaccgt ccgggcgcgg gagatgcctg cagctctggc ctgattgccg gctacctcgg
    17641 catccagaaa gacggctcat tcaaccgcaa ggccgacctc gcctctcgcg tgaagctcgg
    17701 cctcgatctt ggcaaccggg cggccatcgt cgcccagaag accatcggcg acatgggccc
    17761 ggtctgggac gtgggcatgt atttcaaccg cgtgagcgcc ggcaaagaga tcgcacggta
    17821 aaacggtcac acctctgatg caaacgaacc ggccggcccc cgtagttggg ctctgctcag
    17881 gtacccgccc gcctgtggca gccttcggaa cgtccgtcaa ctctgattca tccggtggat
    17941 ggccctgagg tcctccggct ccgcctgaac cgtgatcgcg tcgccggcct caagcaccac
    18001 atcagcactg gggaacagca tcgtttggcc ggatcgggtg tgcgagagca cgaacgcgtg
    18061 caaatgcttg cccacgtcgc ggatcgcccg cccaaccagt tccgcttcct cattcaccgt
    18121 caggttggcc accgccaaca ggcggtcacc cacgtaaaac gaattaacga tcgtcggatc
    18181 gctcgaactg gtggcaaaga gcggcgctgc cagctccgag gtgctgaagg ccagcggcat
    18241 gtcgaacgag tcgcggagcc tcgctgccag ttcctggtcg aacacgcgga tgatcacgcg
    18301 aatgctcgga ttcagcttgc gggcgtcgat ggccatctcc agattggcca agtcgtcatt
    18361 ggtggccagg atgatcgact tggccttggc gacgttgagg tcatcgaaga ccccttcctc
    18421 acgactgtgg cccagccgta ccggaatgcc gttgttgcgc gcaaaagcga ggttgggaca
    18481 atgcgggttc ttctcaagaa cggctactgt ctcgcccaac cggatcagct gctgcagcgt
    18541 ccgcagacca agcttgccca gcccgcacag aacgacgtga ttgttgagcg tcttgcacat
    18601 ggctcgaatc cactcctccc ccgcttcatc gcggcgcagg atgtgatagc tgaaccgcac
    18661 gacggcatcg agaaaaacca ggaagcccag cggcggcaac acaaaataga acatgcgcag
    18721 cagccagtgc tccggaaact cgatcagatg ctccacccag acaagacaat aggtgatgta
    18781 gaacgcttcc gcatagctca gccgtttgtg ggtgtacaga aagtgaaagc agaaggtacc
    18841 gatgatcaac accgtcacga gcagccccaa aaacggcagg aagctcagga tcggcttcct
    18901 gaggtaatgg atgtaagccc ggcaacgtcg ataagggctg cccatgagct ggtactatgg
    18961 ccgactgagt gtcctctgta aatgctcttg agcccgacgg actgtttggg tatgaacatg
    19021 ggtcatgccg gacgtcgctg attcataccg ggaccagacc gccacggtcg ttggaatcga
    19081 cgaggccggg tacggccctc ttctcgggcc gctggtggtg tcggctaccg cgtttgacgt
    19141 ccctttgtcg gtgctcaaga aagtcaccga gcccgccgcc ggtcccgacc tctggagcct
    19201 cctggccgac agtgtcaccc gcaaggcctc taaacgcgag ccacgcttgg ccgtggccga
    19261 cagcaagaag ttgcacggca agggtggcgg aaagcacggc ctcttgctgc tcgagcaggc
    19321 ggccctggcc ttccttgcac aagtgggccc aatgcccgga agcctccggg gactcctccg
    19381 aagcttctgt ccccaagtcc tcgggcaact tgcaggttat ccatggtacc agggggccga
    19441 cgtggaacta cccgtcgact gcggaccgga ttccgtcagg cttcaggccc attcattgca
    19501 ccgttcgctc gccagggccg gaatccggtt tcgtggagcc tgggtggagg ttctgccgga
    19561 ggggcatttc aaccgtcagg tggagacgac ccgcaataaa gcggtcgtgc tcttcggccg
    19621 taccacccgt ttgattcagc gggtgatcga gtctctgggg ccgcgcccgc tcggcgtttg
    19681 gatcgatcga caggggggaa gggtcggcta ccgacggccg ctgatgactg ctttccagga
    19741 tgcccgcctg gaagtgttgg aagagtcacc cgacagaagc ggctatcgtt tgaaccgtcg
    19801 tcccgcgccc ttggtcgtcc gttttgtcaa gcagggggaa aaacatcacc tcgcgatcgc
    19861 cctggccagc atcttcagca agtacgttcg cgagctgttc atgatctgct tcaaccgata
    19921 ctggtgctcc caggtggcag gtttgcgtcc gactgccgga tattacgagg acgggcaacg
    19981 ttttcttgcc gatatcaagg acgccgaagc ttgcagacaa gtagaccggg tcatgctggt
    20041 ccgcgccctg tgaacggccg cgttgacaga cttgcagggc cacagtagag tggcgccaac
    20101 gatacccaag accgttgaga ggtggtatat gccgattgaa aactggtccg acaacgtctt
    20161 gctggttgaa ttggctgacg atccgcagtt cacggacgac ttgaccaccg ttctcgatac
    20221 ggtcgagcaa cgcaccgatc tcgacgtcgt gctgaacttc caggaagtca ccttcctcaa
    20281 ctcctcgaac atcgccaggc tgctcaaact gcgcaaagcg gtggtcatca acaacaagcg
    20341 aaagctgcac ctgtgcgcga tcagtatcca tgtctggggc gtctttctcg tcaccggcct
    20401 cgataagatc ttcgatgtct atgacgatgt cgcaacgggt ctcgccggcc tgcagttggc
    20461 tcagtagtcc cggtcgggtt tcgccgttcg cccttcttca ccggttgtcc cccgcggctt
    20521 gggtccgtcg ccgggccggt cttcttcacg cgccggacgc aactgattaa cctatcaacc
    20581 gcgtttgcgg gaaaccgttc cagggttggt tcattgcggg gagatcattc catgtccatc
    20641 agtcgtcgaa ccgtctcgat cgggttgctt gccctgtttg ctgtcgctcc cctcgcgccg
    20701 gccgcggacg cgccgcctga gggcgagaaa gccgcggtta cggtcgaaac cgacttcgtc
    20761 aagtacaccc tcggtgccga tgcccggagc ctccacttca tcgacaagca gcgcggtacg
    20821 gactattgcc ggcctgtgcc ggtcgcccag gtcgagattg ccgggaagcg gcacccggcc
    20881 ggctcggcga aagccgatgg ggatctggtg gtgctcggct tcgccggcac cgatgcgcgg
    20941 gcggtcctca agcttatctc tcattcccgc tatttcgtcg tcgaggtggt ctcgctggaa
    21001 gggcccggcg tcgaagtgtt cacgttcttc gatctgccgc tcaccctcca ggcccaggcg
    21061 gacgaaccgc tcgcggcatg taccttggcc ctgaatctga aaaccaacgt ccgcgagatt
    21121 ccgcgaccgg tcagtcgtct ggaagcgctg tcctacccgc ggttcggttg tgcgggtgca
    21181 caggcggccg tcgtggcgtg cccgccggac caactccgac agatcttgca gcaggtggtg
    21241 accgatgccc ccgacctgcc ccattcacct ctgggcggac cgtgggccat ggatcacgac
    21301 gtcaacaagg gttcatacct cttcgatttc ggcgacctca acgaaaagac ggtcgacgat
    21361 tggatcaggc tcgctaaacg cctcggaatg aaccagatcg acttccacgg cgggcggtcc
    21421 ttccgcttcg gtgactgccg gccgaatccg gagatctatc cgaacgggtt ggcgagcatg
    21481 aaggcggttg tggatcgcct gcacgaagca ggcatcaagg cgggtctgca cacatatgcg
    21541 ttcttcatcg ccaaggatgc tccttgggtg acgcccgtac ccgaccctcg cctcggcaaa
    21601 gacgcgacgt acacgcttgc ctcggatatc acggctgatt cggccaccgt gtccgtgaac
    21661 gagagtcttg aaggacgaaa cctcaagacg ggtttcttcg aacggaacag cctgacgttg
    21721 cagatcgacg acgagttgat cgtctacgcg ggcctgtccg aggacaagcc atatgctttc
    21781 accaagtgcc agcgaggagc ccacggcacg aaggtcgcgg cacacaaggc cggggccaag
    21841 gtgcaccatt tgaaacagtg tttcttcctc ttcacacccg acggcgactc gacgctgttg
    21901 gaggaagtcg cccagaagac ggccgacact tacaacgcct gtggtttcga catgatgtac
    21961 ctggacgctc ttgatggcga aggcatcctc ggcggcaacg aatacggctg gcactacggc
    22021 tcgaagttcg tctttgagct gttcaagcgg ctcaacaagc cgccggtgat ggagatgagc
    22081 accttccatc accacctatg gtatgttcgt tcgcgcatgg gggcctggga tcaccccaat
    22141 cgcagccaca agcggttcat cgacctgcac tgcgaggcca acgccggctt ggcccggatg
    22201 ttcctgccgg ggcacctggg ctggtgggcg gtcaagacat acagcgatat ccagaacgaa
    22261 cccaccttct cagacgacat cgagtatctc tgcggcaagt gcatcggcaa cgattgcggc
    22321 ttctcgatca tgggcgtcaa ccccggcaac atcaacaccg tccccgtttt cggacgcttg
    22381 gccgagatca tgcgaaacta tgaggagctt cggcacgcga agtacttcga tgaggccacg
    22441 cgaacgaaac ttgccgaggt gggcaaggaa ttcacgctgt tcaaggccga agacggccga
    22501 tggcgcctgc ggccggtgca gtacgccaag cacaaggtcg agagccggga ggattgcgcg
    22561 cggatctggc gggtcgaaaa cccctatcgg cgacagcccg tgcggttgcg gatcgaggcc
    22621 ctgccatcga tctcgcctta tgacaccccg ggcggccttg tggtcgagga tttctccgat
    22681 acgtctgact tctctgagcg ggcggtcaac accggcgtga cggccgatct gcggccgagc
    22741 accgggcagg tcaaggtcgg tgctgtcagt ggagccttca ctgcatccaa ctcgggcaag
    22801 gtgggccgaa ccgcagcctg ggccaaggtc ggcaagaagt tcgccccgcc gcttagcctc
    22861 gaaaaacacg agggcctggg cctgtgggtg cacggcgatg gcaaaggcga ggtgctgaac
    22921 gtgcaactga ccagcccgcc tcatgtctcg cacgggattg gtgaccacta catcaacgtg
    22981 gattttaccg gatggcgcta tttcgagttg gttgagccgg aaggcgagcg ctggcggcag
    23041 tacacctggc cctatggctg ggcctattcg atctttcgcg aacacgtaga cttcaggcag
    23101 gtcgagacca tcagcctctg gtacaacaac ctcccggccg aggacaccgt cacctgctat
    23161 ctcagtccca tccgcgggct ctcggtgttc atgaacaagc tcaaggatcc gtcgatcacc
    23221 atcgggggaa agacacttgt gttcccggtc gaaatggaga gcggcagcta tatcgagttc
    23281 aactcgccgg ccgactgcaa gcactatggc aaggatgggg gctttattgg cgatgtcaac
    23341 ccgcagggag acgtgccaac cctggaggca ggggccaacg aggtccgctt tcactgtgac
    23401 accggcggcg aagcccaacc ccgcgcacga gtcacggtga tcagctacgg cgagccgctg
    23461 tgatgcgagt ctcggtcaac agcaccatca gcagcaccgc ggccagaccc gccagcgcgg
    23521 ccccgatgcc gaatgcccag cccggcccga tcaccgccca gaagacgccg aacagcaggc
    23581 tgcccggcag tgccgccagg ccgaccgcgc cgttgtagac gccgtaggcc gtcccgaggt
    23641 gctggctggg gacgaaatcg gcgaccaggg ctttctccgc gccttcgctc atgccgtaat
    23701 agaatccgta ggccaggaac aacgcccaga actgccattc cgcctgcgcc tgcgaaagtc
    23761 ccagatacac cagcgcatac gtcacccagc ccgccacgat catcggccgc cgacccagcc
    23821 ggtccgagag catccctccg gggaaactga agacgatctt ggacagatgc agcacgatcc
    23881 acaatccgat gaccgccggc agtccaagcc ggaatttttg ccaagcgtaa agcagcaaaa
    23941 acatgtccga gctgttgccc agcgtaaaca gcaccacaac gcccacaaag ggatagaaac
    24001 gccgaggcag gcgtttccat gctccggcag cagtcgccat ccccttgctt tcctgcgccc
    24061 ccgagaccga ccggcgcggc cgaggagcaa tctcacgaac cttcaggatg agtgtcgcca
    24121 ccgccagaag ccccggaatc agggcgatgc cgaacagcca tcgcagagcg gtcatctcgt
    24181 cctgtgtggg cttggcgtcg ctgcttctcc atatcccgta gcccagcata gccatcagga
    24241 tgcacaccga aaccagcgaa ccgagcaccg cccccgcatg atcgagcgcc cgagtgaagc
    24301 tgaacgccag cccgcgaacg tcggccccca cggagtcgcc gatcaacgca tcccgcggcg
    24361 aggtgcggat gcctttgccc acacgatcca gaaatttcaa tcccaccacc tgccagccgc
    24421 cccaagccgc ccccaccagc cccgccaagg ccatcaaagg ccgcaccgcc gtcgaaatgc
    24481 catagcccgc tacaaccagg gcctttcgcc tccccagacg gtcgctgatc cggccggaga
    24541 ccagcttcag cagactggcc gtcgtctctg cgaccccttc caccagcccg acgtacaccg
    24601 gggccatgcc cagcggcacc agcccggcga tgaagatcgg caatagcggg ttcatcatct
    24661 cgctggacag gtcggtgaac aaactcacca gccccagcac caggacgttg ccccgaaaaa
    24721 cacttgctcg ccgcttttgc tggtcgacac caagcgtcac tctttgttct gcgtcgctca
    24781 tcgtcgaaga ctccggcaca caggcatgaa ccgctcggac accgccagtg cgaacaggcg
    24841 ccgcgcgccc agcaccaggg taatcgtcat cgaccgcggc acccgcatga caactacctc
    24901 gaacgagtca cggttttcta ctgcaaaact gcaaacccgt tgatagtcgg ttggccttca
    24961 tgccctgggg ccggcgtcgt gccccctttg ccagcgatcc tccggcagga aatacgccac
    25021 cacggccgca ggcaggaaca gcagaccggc acagagaatt gccggccgat ggtcgcctac
    25081 cttcgagaac aaaccgaaaa acaccgtgcc gccggctgcc acgacccggc cgatgttgta
    25141 gcagaaaccg gcccccgtcg tgcgaagcaa cgtcggaaac aatggcggca gatacatggt
    25201 aaacagggcg aagatgcctt gcgatagccc ggccaggggc aagcatgcca gcaggctccg
    25261 atgatcgcga ggaacgccgt acgccaacgt cagcgacccg aagtaagcca cacataacag
    25321 cagaatgacc cggcggaaac ccaatcgcgt tgccagaaaa ccggccagga agttgcccgc
    25381 gatagccgag gccatcacca ggaacatcgc ctggctcacc aaggcattgc gccgcgcctc
    25441 gctccagcct tgcaggctct ccagcccgtg gaaatgttgc agataccaga acaaaaacgc
    25501 ccagtgagcg gtcagcgtgg tggcacatac gaagaccgtc agtaacgtcg tgcgtcggat
    25561 cgagccacga aagagatctg cgatgccggg ctggtcccgt tcgtgccgct tgaccgcttc
    25621 ccagtgagaa gactccggca ccgcccgtcg aatccagaac acgatcagtg caggcaggat
    25681 gcccaccagg aacacggcgc ggttcggcgc ttccatgcgg gcaatcacgt agtaagccag
    25741 gctggccagc agtacaccgc agttgccgct ggtctggagt acggccgcga cccacggccg
    25801 caaccgcttc ggccaggtct ccgacagcag cgaagccccg accgcccatt cgcctccgat
    25861 ccccagcgcc gccagaaacc ggaaaatcag caggtgccac cacgtctggg cgaagaacga
    25921 gagacccgtg aaggcagcgt aggtcaggat ggtcagcatg agcgtacgag tgcggccaag
    25981 gcggtcgccg atgcgcccga agaacgctcc ccccaaggcc cagccgacca gaaaactcgc
    26041 ctggatccac gaaccatagt agcccgccgc cggatccttg atcgaaacgc gcagcagctc
    26101 ggccacaaac ggcagggcca ccagcgtgta caggtgcatg tccagcccgt caaacatcca
    26161 ccccagccac gcggcgatgc cggctttcca ctgggcggtg gataggtcgc gcaaaccgct
    26221 gtgtgcgttg tcgctcatgc tccaggcttc cgggccgttc tcatccccct gaggcggtta
    26281 cagatgggcg gaaggtatca tcgggcggat cgatttgcca gttgtatgta cctcatcccg
    26341 tcagagcccc tgattccgca cctgcggacc gaccacaaga tcgagccgga ggtcttcagg
    26401 gctcggttcc ccgaacaccc cctgtgctcg gagcgtttcg ccggtttcct ggcggcccac
    26461 aacgtccgca aggtcaacga gtgcctgcac taccgcctgg aagtggccgg ctgcccgatg
    26521 accgcccgct acggcgtcga gcaccccctg atcccccagc ccgatccgac cttcgtctgg
    26581 cccttgacgc gccgcgagcc acgaagtggc atcggtcgcg ccgcgaccgg cgggccttgc
    26641 ccgcagtggc gtgtcaaggg ctggaccgac cccgcccgcg acgtggccga ggccgtcgag
    26701 cacaacgagc gggtcttcct ctacggcccc agcggcacgg gcaaactcag gccaacgagg
    26761 aactgaccac agtcctcacg accaggcgac ttctggctct ctgcgccagg ttggagcgtg
    26821 gcaacaagtt tgcccgggcc ctcgccgtct gcgtcttgaa caaggttccg gccgaggacc
    26881 ggaaagtgat cgaagaaacc ttccaacacc atatcggccc gctgatgcag ggcaaggtat
    26941 gagcgggaat tatgcggagg gagcactctg ggcaattgct ccctccgcac ttcatgcctt
    27001 tggtacgatc acaccgaaca agcagcggcc cagttcttca caggactgcc gtcctgtgcc
    27061 gttactactg ccccacaatg atttcgagtt caggtccgcc aaccatcggg acactcgcca
    27121 tatcctcatt gatgaacgat ccgtcattca gcgtcaggtg gtaggtaccg ggtgttccgc
    27181 tgattgtcag cgagagtgtc gccacagcgg tttggccaac cggcagcagt tgtcgggtcg
    27241 ctccaccgat gctggccgtg gttgtgaaca ggtccccggc tggggtggcg atcgccccat
    27301 caaccgtagc caagctgttc gtcagtgtcc acgctgtcgg cgggtccggc gccagcaagg
    27361 cttccacgtc ggtgccggag tcgatgacgt cgctgccatc cggcgtcatc ctggcccaga
    27421 gccagtccat caccgtcagg tcgtagtaac tgctctggtt gtggccgtcc actctgtggg
    27481 agaagagcac gttatccagt tcggtccagt cggccgagtt gatcgagacg acgttggctg
    27541 catcgacagc cagcctgccc tcgaggcccc agatgggcgc atccgtcgcg actaccaggt
    27601 caaccgcaac cgtcccaccg ccagccggca gtgtcaccga tgttgctccc gttccgtgga
    27661 ccacccaccg agccgagacg tttgccgtcg ggctgagaag acccgcggcc agctgctgtt
    27721 gtagaagtgc cagcaaggtc aattcctgtg tctgatcacc cctttcggcc atgctcatcg
    27781 actggcttat ccccatcgtc atgctgccac ccgagcaaac aaccgtgttg tcgagaagtg
    27841 catgtacaat ggtccagtca gcaccagtgg tctcggtgcc ggtgatcgtg ccgtcagtgt
    27901 cgatatccag acgcatgttg gattcaccgg cggtctggcc gttggcggcc tcgatcgccc
    27961 cttcatcgtc ggtctcttcc acggtaacgt cgccgtccac gtccgccgcc agcatgcgaa
    28021 tcgagcaatc ggcgtcgcac tcaagctccg tccagttcag gctgatcagg gcgtcggaca
    28081 ggtcgatcgt gtagcacacc tgatcgttaa gacgattgtc cgggtcagtc gcatcgaaga
    28141 agagccggaa ggtctgatca ttgttgacga aatcatacga cataggccag ggctggctgg
    28201 catcgctgcc ggtgacgtag acaaaggata ggtccgtcca cgggtaggcc ggctgggcca
    28261 agtccacttc gatgatctgg acgtcgccct gacgcggttc gatcaccgca ctatcaggcg
    28321 ccgcgtccgg gtccagggta atcccgagtg gtccaaccgt gccatgagta cgaacgctcc
    28381 gccaggcggt gatggtgaag tagttctcag gtgtttcgca cggatccagc ggagatgatg
    28441 acgctggagg cggttttgga ttgtaactga tgcccgcccc agtcgcgttg gcttcaaagg
    28501 catcccaatc ctcgatgctg accatccggt catcgttgct gtcaagttcg acgcacaagt
    28561 agtcatccca cagcgactgt tgcggctcct gtcggccgag gcagtactgc cagcgcgcga
    28621 aatcaacctg atcgatatca cagtcatggt cccagtcgta ccgtggctgg cggaatcctt
    28681 cagatcgatc gttctgctcg t
//
LOCUS       DAOTPW010000036        28105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1758_length_28105_cov_5.568485, whole genome shotgun sequence.
ACCESSION   DAOTPW010000036 DAOTPW010000000
VERSION     DAOTPW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 28105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28105
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1758_length_28105_cov_5.568485"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            526..1353
                     /locus_tag="P5316_05020"
     CDS             526..1353
                     /locus_tag="P5316_05020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR84352.1"
                     /translation="MTRGQLHSCHLDFVILVLHLYLLVERSLAISMIIDGHVHIGVGN
                     YFHMKADVDQLLRLADKVGVDKLFVTDLGSLFYDMSEGNDRLARDIAAHSDRLIGYCS
                     VPTPRFGKRAVDEIRRCFEKHGFRGVKIYSHPEAPITEPAAYPLLESAAECGMLILSH
                     TTPDECDHLMQNVPQARLVMAHMGGHPYAFGDWYRAVAVAKKHPNLLLDTASSQIDNG
                     MLEWAVAELGPEKILYGSDMPLLDPFTQMAKVTGAELDDRAKSLILGDNLHRLLGLP"
     gene            1350..2099
                     /locus_tag="P5316_05025"
     CDS             1350..2099
                     /locus_tag="P5316_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR84353.1"
                     /translation="MMRLDINACFGHWPYWDLPHKTAEDLVRLMDRNGIEKAAVLSLR
                     GVLVDWRAGNDETLAAADRHPGRLIPMATLSPFMDGNGDELRRLADTGMRGVRLYPLF
                     HSYPLDSEFTDDVCRAAAERRIPVMIPTRPMMNWRFKTVAIESIGALAARHPSTTFII
                     SGPNYLIECQALVKVMGQCPNVSCEISCLQGFDSVTNLVRQVGADRVLLGTGATLHYP
                     ACNVAKLDHAELSTRQREAIAATNALRMLGL"
     gene            complement(3243..3737)
                     /locus_tag="P5316_05030"
     CDS             complement(3243..3737)
                     /locus_tag="P5316_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRR84354.1"
                     /translation="MSIDLSKDAGLLAGLAGEVRAKTLRLFDDVKDSELMYSPPGLSN
                     HILWYAGHVLWVQDALGIAAITGKSELPVGWAETFGKFCRPVKETKQWPGRGEIRERL
                     VAQLPRLQEVIGSVGKRDLESPSPNKALGPEKPLWYWITHASHDEANHQGEIYLLMKI
                     QRNR"
     gene            complement(4517..4969)
                     /gene="ruvX"
                     /locus_tag="P5316_05035"
     CDS             complement(4517..4969)
                     /gene="ruvX"
                     /locus_tag="P5316_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRR84355.1"
                     /translation="MDTRYLGVDLGTKRIGLAISDPHTGVVSPLSVINTRAGLEQNAS
                     AIIDVAEEYDAGGIVIGLPLNMDGTEGPQARLSKQLAEAIRKACQARRNAATITVHLH
                     DERLTSHAADRRLLGRDLTRKKKKNRQDAIAAQVLLESFLDRLTGKNT"
     gene            5380..6369
                     /locus_tag="P5316_05040"
     CDS             5380..6369
                     /locus_tag="P5316_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR84356.1"
                     /translation="MMTKRERVEAVLGGRRPDCPPVSFWHHFEPHEITGRAALDAHLR
                     HLETYDLDFLKVMNDHHYPRGDVNLVQSVADLRKIKPLPGDAGQLAGQLDLLRGLRRR
                     LDDDLFTCATVFSAWTVLRAWVAPPSDKHGPPKIIGEDQRDDVLTKLLQEDRSAVKAA
                     VRAIGKMLAAFARECVAAGAMGIFLSVRDDWVDRPANGDGVYDEMVMPTDMEILDAVK
                     HAPFNVLHVCGRPMIFERFANYPVAVINWADRAAGPSIAYACEVARPAIAGGVDNLKT
                     LPQGKPEDCVAEVRDALRQAGDRPILITPGCTYDPKAVPAENLKAVVSAARSG"
     gene            6430..6606
                     /locus_tag="P5316_05045"
     CDS             6430..6606
                     /locus_tag="P5316_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRR84357.1"
                     /translation="MKTGTIRGGFGASTTYNLVHGSDSPEWAAVGISLCFNDAEILDY
                     VTPEAGWVAESDEG"
     gene            complement(6873..8303)
                     /locus_tag="P5316_05050"
     CDS             complement(6873..8303)
                     /locus_tag="P5316_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRR84358.1"
                     /translation="MNAFYLPVYHSLACLRRFASTAILFLASSAVTAAAPSSQAADGQ
                     PKTVAAPPATQPDSLTIPLQTTHVDALRADEKDLPLIRDLIARFDTSSLKPPLTQATA
                     SRPAATAPAAEEPLVVRVFSLKHIDPKTVINAITTAFSDPTTRVTAGSSNGVVVRAKE
                     KAMAAIEQLLQKLDAAPDQSQEMRTIIFPLRHAKAAELAPILQRAVPGLRECISWAAA
                     NSLLVTGTPKAIETARSLVAELDAPPSAPPPAPPTNEPITKLFPLEFARPNPALLDAL
                     RAAAGDGSSVVPVPDIRSIIVTARPKNMAAVESIIKQIDRQTEPGHAPAVGLRIRFLW
                     LVSGLPEDKSSPTPADLLPLVEDLQRIGVTGLRLATQNVVEATPGRRFIMKASPLLAN
                     RCDLELEGQFVNETASQPCVELQARVAEYAPYLEPEKNGYKVYERRELCKLETTVVTP
                     LGKAIIAGVTPIDRMTSVFVVQVLPQ"
     gene            complement(8573..9721)
                     /locus_tag="P5316_05055"
     CDS             complement(8573..9721)
                     /locus_tag="P5316_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR84359.1"
                     /translation="MRNRQANTLQREAFTLIELLVVIAIIAMLISILLPSLANVRELA
                     RRVSCGVNLRSIAQACIMYSESNQQGFPTAWQPSTVALSMTSNTAIGSLRGLPDGWMD
                     ADGPAALDNQSNYRAYYRLLLGGPKAYLQPRQLICPTGNRKLGHFSSGTNPSPIVNDA
                     NLKYFAKPKPPVGAEGKWYDFDGSPTAIVNAGTELAEISYSLQMTRVATVNERGIGQT
                     YGVRMTSSQDPRKALAADRNPFSNRIEMLHPNPAGPQASRYNFDPTAQGTGLPAPGDL
                     DGNGSVDAGEWLAALRKRDRSLSSRNHNLAGQNVAFLGGNVKWCRHPLCGADEDLIWT
                     PTSPPQVGGTVQDPLETHVYLLPGRGTPANYQTALSDPAVSTDSLLIP"
     gene            complement(10096..11043)
                     /locus_tag="P5316_05060"
     CDS             complement(10096..11043)
                     /locus_tag="P5316_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84360.1"
                     /translation="MTYRLEPGVQVRVVAPPAAQFDRPKPTQIIVYALPNGNTTDQTI
                     GKRVTSGVDWHYGIQHIGAQTRRMREVIRDENLVTAYVEADGKSWPAWRSKHADSSRI
                     IAQVIDSIAGRFEGMQTSITLTGHSGGGSFIFGYLNGVDQISDRIDRIAFLDSNYAYS
                     DDQHHGDKLIAWLKASPRNHLIVIAYDDRNIMLDGKRVVSPTGGTYRRTTEMIARLRK
                     EIALSEKPLLETLPTGAFPAIAPSGHASALAMSKRYRGLDGQIDIVLVENPQNKILHT
                     VLVGDMSGFIHAITSGTPYENRVAVFGGPVTYEKWIQQD"
     gene            11965..14544
                     /locus_tag="P5316_05065"
     CDS             11965..14544
                     /locus_tag="P5316_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84361.1"
                     /translation="MLQKNQRRTAFLRAATQVLCDPETCAQNAHQSRRKGLKALAAIA
                     AVAAFGLSIQAAYGANEIRYSYYGGGQQIWIPAARTAAVSANYGQAANYVEDLSANDP
                     ISGHAYFFPPTGLPADAWRVSCPSPPGTCVDNQAQADAWVQYEIFPADVPGDISLSGK
                     WYFWTRTQQLYAESDVESNQDSDWLVVNGNPPGFGGSNDKGDIDITDHVPTDAEWYTA
                     VTKGAANEPQREYDRICNDVRFVEWPPGINPKLDGRRPNWVWIGNIIANGNYRLNKNF
                     KLINGRITFRIYEAEASPWNACIDVICWSTIESYIPTDEDFNQALILGQCPVQHKTSG
                     SEPASHDNASNTTMTITGTDLHLVSSVKLVPTGGGAELVGTNLVAGAGNTTLTVQFPT
                     QGAQWGTYDVVTLQPNCATKTLPGAFQLTCATATVFNSIEPSTVSNPQYPLQFRIRGA
                     NVTLLTSVKLVYANEDDPNNPNIPGTMLTPDGDDLLATFNLSCFNGEGGPAGPYHLEG
                     TRSSDCSNPARLSNALWVVKPPTGFACVWQPWAAPWSSLNKGPDLDPDPVGEAYNPTN
                     WDYSFSQNTNLGMVKDTPDGSPTAFHFFWDSQPPDTGTQAGSGGIYQEISVTPGVPIE
                     YSFWWKGQATYQANDNIWYELLLLDGPFNLYYADGYQEHTRQQNNPYMVRKKVGMDSN
                     PFGWEQITDQTPPDQGNYGPRPQTITPVNDVVTVVFKCGRVPPGRLEFFVDKVEVRQS
                     GGPNLVSNANFETGSQVYSCENEWVFKDACESDYWRRSPLTPTVCPNPFADFDNDADV
                     DQADFASFQICYTGPTRPLVANECRCFDNNNDNYIDQIDVIAFERCASGPGVPADPTC
                     DDY"
     gene            complement(14542..14614)
                     /locus_tag="P5316_05070"
     tRNA            complement(14542..14614)
                     /locus_tag="P5316_05070"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14578..14580),aa:Glu,seq:ttc)
     gene            complement(14843..15040)
                     /locus_tag="P5316_05075"
     CDS             complement(14843..15040)
                     /locus_tag="P5316_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84362.1"
                     /translation="MACELKVFNTENRTNQTYICDEDNAGRMVENDFAAKGTGEYTDT
                     SGKKFAIDWTKHRLVGFKRGN"
     gene            complement(15201..15707)
                     /gene="tsaE"
                     /locus_tag="P5316_05080"
     CDS             complement(15201..15707)
                     /gene="tsaE"
                     /locus_tag="P5316_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRR84363.1"
                     /translation="MNQRTINIADVEATRRLGRRLGSALQAGDLVALIGPLGSGKTTL
                     VKGIAAGAGVADSRQVNSPTFVIVNEYETGLSPAPRIYHIDAYRLRGGGDLESLGFDE
                     MLSLGAVLIEWADRVQGVLPSSRLEISIEPVDENRRRFLCRAFGSATRLLDRLGPDER
                     IVRPAVNG"
     gene            complement(15704..16651)
                     /locus_tag="P5316_05085"
     CDS             complement(15704..16651)
                     /locus_tag="P5316_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRR84364.1"
                     /translation="MCKLDAMSRGENALIDWLRRRFPANPARVPIGIGDDMACLRLEG
                     SLLQITTDMLLDGVHFDTRRHIYELIGRKAIACSLSDCAAMACRPRAATVSLALHDGM
                     SMDDVKRLYEGMACIAGEFDCAIVGGDTTSWPGKLAIDVAMLAEPMSPRGPIRRCDGR
                     VGDGIYVSGPLGGSAAGKHLTFTPRLELASRLVLDPELHAMMDLSDGLSMDLHRMCAA
                     SRCDAELSTEAMEEVISPAARAMSQEDGISAMDHALNDGEDFELLVAGGEGLRAEELG
                     LTRIGRLTRPSQEGRPTICLCDRLGRFSYIEPRGYEHFR"
     gene            complement(16856..17317)
                     /locus_tag="P5316_05090"
     CDS             complement(16856..17317)
                     /locus_tag="P5316_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84365.1"
                     /translation="MTKWQYWSPLLVGGVIGEVYGLQASMNSGGNPLITMGLLPLASA
                     LSWAILLQLAMFAAQALFAAVLPVPFGKSIRGTQCRVIGFLMLLGSLITLLFKLSGAF
                     VYLGGQRHTLTSLWQIWFLGWAVVAALCFPAALVIYLYSLPMAESDFQAED"
     gene            18439..19554
                     /locus_tag="P5316_05095"
     CDS             18439..19554
                     /locus_tag="P5316_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRR84366.1"
                     /translation="MTRREEHQTKHRRVVEYMESHALDAVVLAQRPNFAWYTAGGLNH
                     VGTADSTGIAALLITRERTICLTNNIEAPRLADEELADLDIEVKAASWFDEGALKRLW
                     LAEVGGLRTACDVPFPALPGGVCCLDTDFATLRWVMTEGEIRRYRILARDVAECLEVA
                     CGQAEPGMTEFGLAARIAQTALAKGIRTPVLLVAADDRIRHYRHPIPTGRVFERYGMG
                     VLGGERHGLTVSVTRLFSFGPIDPELRRKHEAVCRIDAAMMAATRPGKAMGEVLAVAQ
                     EVYAQQGFTDEWTFHHQGGLTGYLGREIRVAPGCLIAIQGHQVFAWNPSIAGTKSEDT
                     LLVGPGRNEILSLTGGWPTMPFAVQGQQYCRCCVREL"
     gene            19853..20551
                     /locus_tag="P5316_05100"
     CDS             19853..20551
                     /locus_tag="P5316_05100"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRR84367.1"
                     /translation="MNNTNTAPVQSRGEATRTDILQVARQLFSKHGYHRTGIADIQEA
                     TGLTKGAFYHHFHSKEDLALAVLERTRQEYAEQLFAPAMKQHTAAERLEALLDQAAEL
                     NNRPEWCNCQMLATLTAELTPADGRLREAVQDIYDDLQDRCRKLLREARDAGHTSDQV
                     DPDTGAQWIANTLTGLSLAKKLGIARVPATNLIDAMKRTLIKRSGRVKGQASGNGESS
                     KSQPRTIPGGRSDA"
     gene            20548..21054
                     /locus_tag="P5316_05105"
     CDS             20548..21054
                     /locus_tag="P5316_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRR84368.1"
                     /translation="MNGTAVLKAQLDMSRQWLMALLADVEGAEVTAPTPNGGNHPLWA
                     LGHIIHSEAGIVNGFVLGRPSPLARWDRLFGKGSRPVNDISVYPSLTELRAEFDRVRG
                     ETLNVLSEMSESDLDRPSKAPTHLSSLFGTIGQCFAIIGLHACFHAGQIADARRAAGK
                     KPVFDSQN"
     gene            complement(21235..23109)
                     /locus_tag="P5316_05110"
     CDS             complement(21235..23109)
                     /locus_tag="P5316_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR84369.1"
                     /translation="MTHVKNVIRLPKRSLLVRECWAVGIVCLLAGCPSNDGNGGPLPN
                     GQANVIGSVKDVAGRRIANAYVACGGVTTTTDNEGMFYLDLPVGQEHIVVISCEGYVT
                     AGVRVDVNETGSSYLPVTLMPMAEPQSLNASDGGVVTGARNASITVPPGAFVNASGLP
                     VTGNVDVRLTPFDPATAAELAAYPGELRGLTLTGETLPLVTYGVLDITVTQNGQPLQI
                     AQGQTITVQVPAPSKGDKPDTSEVWIFDAATSLWVQSEHGDAIYDPQTDSYIATIGHL
                     SPCNIDRPIVPTCIWGLVKDAQGNPVAGAFVQAIPESAGRISSDYTDMYGYFCMYVER
                     NTDMRIEVWTPISDSCPSAMRNDSYCVTTRSIHSGSGLAAGGYPADCSANCTQVPVIT
                     TEDVDPGPIDEAACVVASIADNPFWNTCASGLGDFYACYAPQGACFYEIDPFNPFGAG
                     FVLEFENGSKMESEFSIFQGPVTRVYGPTAKGNPLCGTITGNDSETTITTASGSSYTV
                     SVSESGRMEMTCSGGFSFVLTPEQVEFLSGCNGSSSNDGSGVACKAKPGTMGAACTFD
                     SDCTTPGLTCCGPIGGEQTCQIATFCDLLCDNDLDCEFPSICCSAGGYNMCMPAQACQ
                     "
     gene            23343..23525
                     /locus_tag="P5316_05115"
     CDS             23343..23525
                     /locus_tag="P5316_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84370.1"
                     /translation="MRRGFSVSTAVVGTFKPGVLLQAAFNPSISPPHRHGMNPKTSLE
                     AYLAGAAAVRKSIFVG"
     gene            complement(23540..25738)
                     /locus_tag="P5316_05120"
     CDS             complement(23540..25738)
                     /locus_tag="P5316_05120"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRR84371.1"
                     /translation="MLMRAGIVAVAAMGLVDSRLAAQGFIDVNVKPAPDHEVRYTSGT
                     TIYVEGLVEDRWVGRYWTPSGRINLPYQRWAEDAFELQVRDDPAPDAPLIPLSKGWRW
                     VSATEAQGTNPSRRHFVVELSREAPPVTVRVHTMLDGTPVLTRWLEITNNGRKPLALM
                     TVSPWAGRLWAQTNARLLPPHGIEHDYTLGYFTRQDQGFEGWLEWKPLPAGDTVIQSL
                     LGQAFDAPFFIVRNEARGEYFVGHLAWSANWHMTFRCEQDGALHFGIGPSAGCAQRIL
                     GQDETVQTPAVHLGHIEGDLDSAVQAVHDHLRRSVLPAYPRERACLVQYSATGDQGYL
                     ANEFGDTAGMNESNILANIDLAAAIGADVFIVDAGWWDNPGDWVPSPARFPHGLEPIV
                     DYAHKKGLLFGLYVEIERVNAWNVGNDIGTSKVAREHPDWVGPRAILDLTKPEVAAYV
                     ESELARLIDQYKLDLYRLDYNPLPTSEGPSATRHGFTEDNYWRYYEAFYGLFERIRAK
                     YPNLILQQCAAGGARNDLGTMGRFHEHYLTDGLTMPKVLQNYSGQSLALPPEVFVIGL
                     AEGAHGVNGSGHMDTYLRNVFTLSTPWLLLGVAPSLGELSPRQRERYAHYVNLYKTFI
                     RPILPVCRMYHHAPVSATGGVTSSGWFAMEYTAPDRGKGWATIVRIGTSDTDTYLFKP
                     RGLDRGKTYRATFDSTGETAIRDGMEWVRDGLPVRLEAMMSSELLLFEAQ"
     gene            complement(26206..26850)
                     /locus_tag="P5316_05125"
     CDS             complement(26206..26850)
                     /locus_tag="P5316_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84372.1"
                     /translation="MKPIFIGMALLFMVSASAHADLVYTFNSDAEGFQNVSWQANAPA
                     GWGNTPTVMQTHTVGGWQMQMTKEFSWGPGGGSANQQLAMQALANSGTAKIAFDVMVD
                     GTSFPPASGVWYQFNAVGNSDGTQGWTQIDKLIDAWQNVGQSDLRTWHFELTFAQMGW
                     QPGDTWFQFWTGSNSAAENPVNFYLDNVIITPEPAALTLLGLCGLVLIRQRRIG"
     gene            complement(27152..>28105)
                     /locus_tag="P5316_05130"
     CDS             complement(27152..>28105)
                     /locus_tag="P5316_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84373.1"
                     /translation="DVLRQTRMFDKIWVGTETRDPDNEKWGRKSQYEAYFIMRWLATL
                     GGDKCGGGWFDPYGTIPAVYLEQGRQTVLAGAREVLLFCYGSLQSPEGQANVQAFRRE
                     FPQLFELAALLRGKTPRGIAAPKPPDSPGGSNRYIYDYIGLLGLPLVPAVEIRADAAA
                     MFLPLQASADAGLASKIATMVAAHKPLLLTRQLAEKLAGSVDWAVPEISILDVPNDPW
                     KLMDMPAETLQRIRDRMTAPFGVQVQCPTRVALYLFEGNVAAVESFNDRPAEVLLTVA
                     GMKAPRLELSIPPQSAEIAVRGDGMRLLLAPRSLAVVKWAD"
BASE COUNT         5350 a         8263 c         8870 g         5622 t
ORIGIN      
        1 ctgacggaaa ggggtgagct gcggtactgg atcatcggtg actggcgtcg cggccggcaa
       61 caccccatcg ccccgacagt ggagaactgg cggcgggagg tcgaggctct ggcgagtgga
      121 ctgaggcaaa aagtcagcat ccaggtgggc gagtccgagg aaccgaggtc ttgaggaaat
      181 gacgaatgac gaagcacgaa tgtcgaatga atgacgaagc acgaatgacg aaaggtatgc
      241 ggcttacgtc cagcctgctt gttcagggtt ggtcatccgg gtttcattcg ccgttgaggt
      301 gcaattcgcc gaacacgcca tcacaagccg ccccggcggg ggcgaaggta tttagccaag
      361 ggcgtaagcc cctgggttct tgtaaacgga tggtgttttc taccgcccct tcggggctga
      421 tcggtctcgg cggcagttgg acgaggggct tgcgttcctg gctacatagc tgtgcccctt
      481 cggggcaacg cggccgctgg tatgtagaaa ccacgagaga gccgtatgac gcgaggtcaa
      541 ttacactcct gtcatttgga cttcgtcatt ctggttcttc acctgtacct ccttgttgaa
      601 aggtctctcg caatctcaat gatcatcgac ggccatgtcc atatcggggt cggcaactac
      661 ttccacatga aggcggatgt ggatcagctt ctgcgtcttg cggacaaggt gggcgtggac
      721 aagctgttcg tcaccgatct cggttcgctg ttttacgata tgagcgaagg caatgaccgg
      781 ctggcacgtg acattgcagc ccattcggat cggctcattg gctactgttc ggtgccgacg
      841 ccgcgattcg gcaagcgggc cgtggacgag atccgccgct gcttcgagaa gcacggcttc
      901 cgcggggtga agatctactc gcacccggaa gccccgatta ccgagccggc cgcctatccg
      961 ctgctggagt ctgcggccga gtgcggcatg ctcattctct cacacaccac accggacgaa
     1021 tgtgaccacc tcatgcagaa cgtgccgcag gcccggctgg tcatggccca catgggcggg
     1081 cacccgtatg ccttcggcga ctggtaccgg gcggtggcgg tggccaagaa gcacccgaac
     1141 ctgctgctcg atacggccag ctcgcagatc gacaacggca tgctcgaatg ggcggtcgcg
     1201 gagctcggac cggagaagat cctgtacggc agcgacatgc cgctgctcga cccgttcaca
     1261 cagatggcca aggtcacggg ggcggaactg gacgaccggg ccaagagcct gatacttgga
     1321 gacaacctgc accggttgct cggcttgcca tgatgcgatt ggatatcaac gcctgtttcg
     1381 gtcactggcc ctattgggat ctgccgcaca aaactgcgga ggacctggtc cgtttgatgg
     1441 accgcaacgg catcgagaaa gcggccgtgt tgagccttcg cggcgtgctg gtggactggc
     1501 gggcgggcaa cgacgagacg ctggcggcgg ccgataggca tcccggtcgt ctgatcccca
     1561 tggcgacgct cagtccgttc atggacggca acggcgacga gctgcggcgc ctggccgata
     1621 cgggcatgcg cggcgtgcgg ctgtatccgc tgtttcatag ctacccgctg gatagcgagt
     1681 tcaccgacga cgtgtgccgg gcagccgccg agcggcgaat ccccgtgatg attccgacgc
     1741 ggccaatgat gaactggcgg ttcaagacgg tggccatcga aagtattggg gccctggcgg
     1801 cccggcaccc gtcgacgacg ttcattatca gcgggccgaa ctacctgatc gagtgccagg
     1861 cattggtcaa ggtcatgggg cagtgtccca acgtgagctg cgagatctcg tgtctccagg
     1921 gcttcgactc ggttacgaat ctggtccggc aggtgggtgc ggatcgcgtt ttgttgggga
     1981 ccggcgcaac tttgcactac ccggcgtgca acgtggccaa actcgatcac gcggaactga
     2041 gcacacggca gcgggaagcc atcgcggcaa cgaatgccct gcgcatgctt gggctatgac
     2101 cggatcgtcg ctgcggtcgc tctcaatgtg tttcacgacg gtgtgggaca ggcttccagt
     2161 ctgtcacagg agattggtcc cacaaagcag gatggggttc gaccgagctc gccgctctgc
     2221 aagccggggg ccacaaggtt attcgcggct caatcggctt cggagggtac cagggaattc
     2281 cgattggccg tgccagtgac gtgctccggg cggtgtccga tccccggaaa gacggcgtcg
     2341 ccgtcggctg ttgagcaatg tccggcgatg gggccggcag gctccacccc gggcatgtta
     2401 cccgagcccg ccaggcccga tctcgccgga tcaaaccttc ccgttgctac gacagaaacc
     2461 acgcggagcg gtctgcccgc ctcgcttgtc tgacgtatct tttgcccatc aaatgcttct
     2521 tgccaggcgc tcctgcctga cagtccgacg gccggattgc cgggcagtct ttgttcagtc
     2581 gaggacgaac acccgtgctt cggcaggccg agagtattcc tccatctcgg caatgctgga
     2641 aatcgggagc ggttgcgcct gctcccgggt cttggacggc acataggcgc gcccacactg
     2701 acggcagaac tgcggtctgc ccctacgccg gaccggaacg atctgcgggt gttcacaatg
     2761 cggacaaagc aggtatagcg gcacagcaaa cactccttct cactcgcggg tacctgccgg
     2821 gctcgccctt gcttccctct tgcccaggct cttgcggccc tgagcctgac cggcaggccc
     2881 caatggggaa tctacggaat attggacacc tatttcggcg aaaggttgca cctcttctga
     2941 gggattcttt ccatccgacc ggaagattgc aaaggattgc caaaaaagga gttatcggcc
     3001 ataggcatcc tctcgtcaga gacgctggac ggtctggtcg atcacaagga taggtgccga
     3061 cgatggttat ggaatatttt caaaaagtga aacattttcg cggtcggatc gggaaagctg
     3121 gctggcacaa ctggcgggcg cgagctcggg tggacggaaa gccgacagga ggttttgctg
     3181 tcgtatgtcg gctcgggcac tgagattcct cctgggagcc aagggtggcc gcagagcaag
     3241 aatcatctgt ttcgctgtat cttcatcaac agatagattt caccctggtg gttggcctcg
     3301 tcgtggctgg cgtgggtgat ccagtaccag aggggctttt cggggccgag ggccttgttc
     3361 ggtgatggac tctccaggtc gcgtttgccc accgaaccaa taacctcctg caaccttggc
     3421 aactgggcga cgaggcgttc gcggatctcg ccgcggccgg gccactgttt ggtctctttc
     3481 accggccggc agaacttgcc gaaagtctca gcccagccga ccggcaattc gctcttgccg
     3541 gtgatcgcgg caatgcccag ggcatcctgc acccacagca cgtgcccggc gtaccacagg
     3601 atgtggtttg acagccccgg cggcgagtac atcaactctg agtctttcac atcgtcgaac
     3661 agccgcagcg tcttggcccg cacttcgccg gccaaccccg ccagcagccc cgcgtctttg
     3721 ctcagatcga tcgacatggc ggcgtttctc ctggcaccga agcctgcgct atccgacaac
     3781 acaccatgca cattatagcc ctgccgggag ccacagcgtg tctctggtcg ctcttcgggt
     3841 cagagagaag cactgtcaga gtgagacgca acggtctgct gcccaatctg ccggtgggaa
     3901 gatgatcgac cccggcgttg catacggcag cgccggggaa tgcggaattg tgagtgtgga
     3961 attgggaatg ggctgtcgtt cgccgtcgcc catgaacctc accgccggcg aactccggaa
     4021 acagcgatga ttcggcacga aacgcccgat ctacaacggc cttcatggat acctgcggcg
     4081 atgttcatgc aactgggggc ttcagtccgg ctgagcggcg gtggaagtgc gggctgaacc
     4141 ggcagcccga ggggattctg caaccgtcgt taccgccgag agaagcggaa acccgtcgct
     4201 cgcgcacatt catcgggctt tgagccatag catgtccgag attcattaga agcatcctgg
     4261 cgtctggctg ggttgtcctc agtacctcgc aaggaagccg tcaggattgc agaacagtcg
     4321 cgaaaacaac ttgacggctg ctacaccttc gtctacattc cctacatctg taggataaag
     4381 caggagacga gaggagaact gagcgagaag ggaatggcgt ccaggtggct ggtcaaataa
     4441 accgtcctga cacttttgtg tcgcatcctg acactttttt tgttgagcag gcggttcttg
     4501 cagttctggg tgttgctcac gtgtttttcc cggtcaggcg gtcaaggaac gactccagga
     4561 gaacctgagc ggcgatcgcg tcctgtcggt tttttttctt cttgcgagtc aaatcgcgac
     4621 cgagcaaccg tcgatcagcg gcatgggacg tcagacgctc atcgtgcaga tgaacggtga
     4681 tcgttgcggc gtttcttcgt gcctgacagg ccttgcggat ggcctcggcg agctgtttcg
     4741 agagccgtgc ctggggtccc tcggttccat ccatgttcag cggcaggccg atcacgatcc
     4801 cgccggcgtc atattcctcg gccacgtcga taatcgcgga cgcgttttgt tcgaggccgg
     4861 cgcgggtgtt gatgacactc aggggtgaga cgacgccggt gtgcggatcg ctgatggcca
     4921 ggccgattcg cttggtgccc agatcaacac ccagataacg ggtatccatc gttcttcctt
     4981 gcaggaaagg ctgtcttgcg tacgaaagat catgttaagc ggcatgccgg agcgatacaa
     5041 acgttcgagt ggggggcgcg gcggcctgag tgcgatgatc gggtctcccg cgacttgcag
     5101 ggcgatgatc cttcgaagca agcgtttcgc caagccggac gatgcggctt gacggggaga
     5161 cgggatagag gcagcggcga ctcggaggca ggcctgcaag ggatggcgca aacgctcacg
     5221 agagagtcct cgtattggca tcgtggcatc tattattcgc aggattgcgg caggcaactg
     5281 ctatggggtg gcaagaacgc gggcgctcgt ttcccattcc ccgacggcgg tgcttgtcag
     5341 aagcttgacc ggggatctcg tcgtgcctag attcctcgca tgatgaccaa gagggaacgc
     5401 gtggaagcgg tgttgggggg gcggcgtccg gattgtccgc ctgtgagctt ctggcatcat
     5461 ttcgagccgc acgagattac cgggcgggcg gccctcgatg cccatcttcg tcacctggaa
     5521 acctatgatc tcgatttcct caaggtgatg aatgaccacc attacccgcg aggggacgtg
     5581 aacctggttc agtcggtggc ggacttgcga aagatcaagc cactgccggg cgatgcaggc
     5641 cagttggcgg ggcaactcga ccttctgcgg ggcctccgca ggcggctgga tgacgatctg
     5701 tttacctgcg cgacggtttt cagcgcgtgg acggtgttgc gagcctgggt ggcgccgccg
     5761 agtgacaagc acggcccgcc caagatcatt ggagaagacc agcgtgacga cgttctcacg
     5821 aaactcttac aagaggaccg gtcggcggtg aaggctgccg tgcgggcgat cggcaagatg
     5881 ctcgcggcgt tcgcccgcga gtgtgttgcc gccggggcca tgggcatctt cctgtccgtg
     5941 cgcgatgatt gggttgaccg cccggccaac ggcgatggcg tgtatgacga gatggtgatg
     6001 cctacggata tggagattct ggatgctgtc aagcacgcgc cgttcaatgt gctgcacgtg
     6061 tgcggtcgtc cgatgatctt cgagcgattc gcaaactacc cggtggcggt gatcaactgg
     6121 gcggatcgtg ccgcgggtcc gagcatcgcc tatgcgtgtg aggtggccag gcctgccatc
     6181 gccggcggcg ttgacaacct caagacgctg cctcagggca aacccgagga ttgtgtagcc
     6241 gaggttcgcg atgcccttcg gcaggccggc gatcgcccga ttctcatcac tcccggatgt
     6301 acatacgacc ccaaggccgt gcccgcggag aatctcaagg ctgtagtgtc ggcagcccgc
     6361 agtgggtgac ggcggggctt acttgttccg acttcctgta aaacgggagc acgagcgaca
     6421 ctccagccag tgaagaccgg caccattcgc ggtggctttg gggccagcac cacctataac
     6481 cttgtccacg gcagcgactc gccggagtgg gcggccgttg ggatctcgtt gtgtttcaac
     6541 gatgcggaga tcctggacta cgtcacgccc gaggccggat gggtggccga gtcggacgaa
     6601 ggctgaaggc cgaaggaccg gggatgcagg caaaggctcg ccccaggcga ctcgccgtgg
     6661 cgagacgaag gatgaaggcg gagggatgaa ggcagtcttc gacaagctca ggccggggca
     6721 ggatgaggca tgaattgaga ccccgcggaa gacggttgct ctgtgatttc gcgccgacct
     6781 tcgtcttgtg gccttctcac cgaaccccga accccgaagg ctgcttgccc tgcgaagctc
     6841 aaggagcgaa gcagggaacc ctgacccctt gcctactgcg gcagcacctg cacgacgaac
     6901 acgctggtca ttcgatcgat aggtgtcacc cccgcgatga tcgcctttcc cagcggtgtg
     6961 acgactgtgg tctccaattt gcatagctcc cgtctctcgt acaccttgta gccgttcttc
     7021 tcaggctcga gatagggggc atactcggcc accctggcct gaagctcgac gcagggctgc
     7081 gaagcggtct cattgacgaa ctggccttcg agctccaggt cgcaccggtt tgccaacagc
     7141 ggactggctt tcatgatgaa gcgtcggccg ggcgtggctt cgaccacgtt ctgggttgcc
     7201 agccgcagcc cggtcacgcc gattcgctgc aaatcctcca ccaacggcag cagatcagcc
     7261 ggcgtcgggc ttgacttgtc ctcgggcaga ccgctgacca gccagaggaa tcggattcgc
     7321 aaccccacgg ccggtgcatg gccgggctcg gtctgtctgt cgatctgttt gatgatgctc
     7381 tcaacagcgg ccatgttctt gggtctggcc gtgacgatga ttgagcggat gtcgggaacc
     7441 ggcacgacgg aactgccgtc gccggcggcc gctctcaggg cgtcaagcag ggccgggttg
     7501 ggccgtgcga actccagagg gaagagcttt gtgatcggct cgtttgtcgg cggtgcgggg
     7561 ggaggtgccg agggtggggc atccagttcg gcgaccaggg agcgagccgt ttcgattgcc
     7621 ttgggggttc ctgtgacgag caatgaattg gccgccgccc acgaaatgca ctcgcgtagt
     7681 ccgggtaccg ctcgttggag aataggggca agctcggccg ccttggcatg cctgagcgga
     7741 aaaatgatcg tcctcatctc ctgggattgg tcgggagccg catccagttt ttggagcaac
     7801 tgctcgatgg cggccatggc cttctctttg gcccgcacga cgacgccgtt gctgctgccg
     7861 gcggtgaccc gcgttgtggg atcgctgaat gcggtggtga tggcgttgat cactgtcttg
     7921 ggatcgatgt gcttcaggct gaatacccgc acgacaagcg gctcctcggc agccggagcg
     7981 gtcgccgccg gccggctggc agtcgcttgg gtaagcggcg gcttgagaga actcgtgtcg
     8041 aaccgggcga tgagatcgcg aatgagaggc aggtctttct cgtccgccct gagggcatcg
     8101 acgtgcgtcg tttgcagcgg gatggtcaac gaatccggct gcgttgccgg aggtgcggcc
     8161 acggttttcg gttgcccgtc cgctgcttgt gacgaggggg ctgccgccgt cacggccgag
     8221 gaggctaaga acaggatggc cgtcgaagcg aaccttctca agcacgccag gctatgatac
     8281 acaggcaagt agaatgcatt catgtcgttt cgcctcccga tttcgacggg cggccgtgga
     8341 gtctgcgcca ccttggggaa tatactcgtg acggccgttg gagattgcac cctttcccga
     8401 acgcctttcc cgactcgcac ccacttcgag cgatccggtt tcggaccaaa ggcgacatta
     8461 tagcagactt acgcctctgt gtgaggtctc cggcagcaac gttgatgtcc gagcgtccgt
     8521 attgacccgc gctgctcttc cggtggaggg cctccggctg cgtgaccgga ggctatggga
     8581 ttaacaggga gtcggtactc acggccgggt ccgacagcgc ggtctggtag ttggctggtg
     8641 ttccccggcc gggcagcaga tacacatgcg tctcaagtgg gtcctggacg gtcccgccga
     8701 cctggggtgg actcgttggc gtccagatca gatcttcatc agccccgcaa agcggatgcc
     8761 tgcaccattt aacgttgccg ccaagaaagg cgacattctg ccccgcgagg ttgtggttgc
     8821 gagagctgag actccgatct cgttttcgca atgcggccag ccattcaccg gcgtccacgc
     8881 tgccgttgcc gtcgaggtcg ccaggggcgg gcaggccggt gccctgggcg gtcggatcaa
     8941 agttgtatcg tgaggcctgc ggaccggctg ggttggggtg caacatttcg attcggttcg
     9001 agaagggatt gcgatctgcc gcaagggcct tgcgagggtc ttgcgagctg gtcatcctga
     9061 ccccgtaggt ttgcccgatt cctcgctcat tgaccgtcgc cacgcgcgtc atctggaggg
     9121 agtagctgat ctcggccaat tcggtgccgg cgttgacgat ggccgtgggc gagccgtcga
     9181 aatcatacca cttgccctcg gcgccgaccg gtggcttggg ttttgcgaag tacttgaggt
     9241 tggcatcgtt gacgatcgga ctgggattgg taccggacga gaagtggccg agcttgcgat
     9301 tgcccgtcgg acaaatgagc tgccgcggtt gcaggtacgc ctttgggccg cccaggagaa
     9361 gccgatagta ggcacgataa ttgctctggt tatccaatgc cgccgggccg tcggcgtcca
     9421 tccagccgtc gggcaggcct cgaaggcttc cgatggcggt attgctggtc atgctcaggg
     9481 cgaccgtcga gggctgccag gcggtcggga atccctgttg gttgctctcg ctgtacatga
     9541 tgcaggcttg ggcgatggac cggagattca cgccgcaaga cactctccga gcaagctcgc
     9601 gaacgttggc cagtgacggt agcaggatgc tgatcagcat ggcgatgatg gcgattacca
     9661 ccagcaattc aatcagcgtg aaagcctcac gctggagcgt attggcttgc cggttcctca
     9721 tgatatgcct cctttccaga aaagcgcgca ccagttcccc gaacaatcgt cggcatacgt
     9781 gccgcgcgga ctgctttcgc actcggttca gcggccggtc gaaccgccgg tccggcgaat
     9841 cgacattcca ggccacttct cttcgttgtt ttctgccata aaaacgctgg catttggcgt
     9901 gccatcgatg aggccccgta aaccggcagc ccagtcgtcg gctgggcgag gggcagtcgc
     9961 acgaagtggt cgaaatgaac catgtcgtgg tccaaatcaa ccagcgtcat cctgggttga
    10021 gtggtgcgag ggcgccgggt cctggcgggc ggccatcaga aagagcttgc tcacgggcgg
    10081 aggttgccga cgcagttaat cctgctgaat ccacttctcg taagtgacgg gtccgccgaa
    10141 gaccgcgacc cggttctcgt aaggcgttcc ggaggtgatt gcgtggatga agccggacat
    10201 atcgccgacc agtaccgtat gaaggatctt gttctgtggg ttctcgacga gaacgatgtc
    10261 aatctggcca tcgagcccac ggtaacgctt gctcattgcc agagcggagg cgtgcccgga
    10321 cggggcgatg gcgggaaacg cacccgtggg cagcgtttcc agcagaggct tttcggacag
    10381 agcgatctct ttgcgcaacc gggcgatcat ctctgttgtt cggcggtacg tgccaccggt
    10441 cgggctcacc acgcgtttgc cgtcgagcat gatgttccgg tcatcgtagg cgatcacgat
    10501 caggtggttt cgtggcgacg ccttgagcca tgcaataagc ttgtcgccgt ggtgctggtc
    10561 gtcgctgtag gcgtagttgc tatcgagaaa cgcgatgcgg tcgatgcgat cggagatttg
    10621 atcgacgccg ttgaggtagc cgaagatgaa gctgccgccg ccgctgtggc cggtcagcgt
    10681 gatgcttgtt tgcatgccct cgaaccggcc ggcgatcgag tcgatgactt gggcgatgat
    10741 ccggctgctg tcggcatgct tggatcgcca cgccggccag ctcttgccgt cagcctcgac
    10801 gtaggcggtg accaggttct catcacggat gacctcgcgc atccgccggg tctgggcacc
    10861 aatatgctgg atgccgtaat gccaatcgac gccggatgtc acgcgcttgc cgatggtctg
    10921 gtcggttgtg tttccgttgg gcagggcata gacgatgatt tgcgtcggct tgggccgatc
    10981 gaattgggct gccgggggag ccacgacacg gacttgaacg cccggctcaa gacgatacgt
    11041 caagaccccc tcaccgaagt gccggtcagc cctgaagccg gcggatgcat tgtgaggtcg
    11101 agtcagggga ctctgaacat ctaacgaggt cgggttcggt aagctccccc tttggggggg
    11161 ctcggcaatg gcgggcattg ccgacgcagc cgcaaggatg cccaggagtg tcgttccgac
    11221 ccgcatgatc cagtcggtcc gctcgtcgcg ctgtgtcatg tccgcacgct aaccctgttc
    11281 cccgcaggag tcaaaaccgc ccatcaccct cccgagaaat ccgtgatccc cgaccgtcgg
    11341 ggtcagcggt tcagggccag tttcgggttc ctcggtcacg atttagagcc ttgcggcggg
    11401 atatctgtgc ggtttgcgcg catgtttctg gatcacagag cacttgggtt gcggcccgca
    11461 aaaaggcggt ccgccttagg gaaaacacct gatatgccca aaacgttcgc catatccgca
    11521 ctgcaagacc ccaaaaaaac gggacttcag gctttcttgg caggtcctgc cgatggtacc
    11581 atagagtatc agcagggtca gcctgctata atgtgtcgcg caaatgtctg gtttcgatct
    11641 tcgtgccctt ccgggcagtg tgtgattcta ttcttggagg tatgaggccc ctggccgaga
    11701 aggttgggcg ttgaatcgtc cggccgctgc tgcgctcgtt cgtctttggt cggtcgtcac
    11761 atgacccatc ttcctcggtc ccgtcgggga ggttcgcaga tggcgtagga tggcaccaac
    11821 atccgcttga atgctggccg atcgattggc acttggcatc ggcttgtcct gaagcagcgt
    11881 gcgaccgggg tgcgtgcccc gacaaaaccg gagagtgcgg aatgaggttt cttgttgaag
    11941 aagtcacata aggaggacta ggagatgctt cagaagaatc aaaggcggac cgcctttttg
    12001 cgggccgcaa cccaagtgct ctgcgatcca gaaacatgcg cgcaaaacgc gcaccagtcc
    12061 cgacggaagg gactaaaggc tcttgcggca atcgctgccg tggccgcctt tggtttgtcg
    12121 atccaggcgg cgtacggagc caatgagatc aggtattctt actacggagg cggtcagcag
    12181 atctggatac ccgctgcgag aaccgccgcg gtcagcgcca actacggaca ggcagccaat
    12241 tacgtcgagg atctgtcggc caacgatccg atcagcggcc atgcttactt ctttccgccg
    12301 accggactgc ccgcggacgc ttggcgcgtt tcctgtccga gtccgcccgg cacctgcgtg
    12361 gacaaccagg ctcaggccga cgcatgggtg cagtacgaga tctttcccgc cgatgtgccg
    12421 ggagatatca gtctcagtgg caagtggtac ttctggaccc gaacgcagca gctttatgcc
    12481 gaaagcgatg tggaatcgaa ccaggactct gactggctgg tggtcaacgg caacccgcct
    12541 ggtttcggtg gaagcaacga caagggcgac atcgacatta ccgaccatgt tcctacggat
    12601 gcggagtggt acacggccgt gaccaaagga gcggcaaatg aaccacaacg agagtacgac
    12661 cgaatctgta acgatgtccg gtttgtcgaa tggcctcccg gaatcaatcc gaagctggac
    12721 ggccggcggc ccaactgggt ctggatcggt aacatcatcg ccaacggcaa ctatcgactg
    12781 aacaagaact tcaaactcat caacggcaga atcacgtttc gtatttacga ggccgaggcg
    12841 tccccctgga atgcctgtat cgatgttatc tgctggtcga cgatcgaatc gtacatcccc
    12901 acggacgagg acttcaacca agctctgatt ctggggcagt gtccggtgca gcataagacg
    12961 tccggaagcg agccggcatc gcacgacaac gcaagcaata cgacgatgac gattaccggg
    13021 acggatcttc acctggtcag cagcgtcaag ctggtgccga ccggcggcgg cgccgaactc
    13081 gtgggcacca acctggtcgc cggtgcgggg aacaccacgt tgaccgtcca gttcccgacc
    13141 cagggtgcgc aatggggcac ttacgacgtg gtgaccctac agcccaattg cgcgacgaag
    13201 actctacccg gcgccttcca gctcacctgt gccaccgcga ccgtgttcaa cagcattgag
    13261 ccgagcacag tctccaatcc gcagtatccg ctccaattcc gcatcagggg ggcaaatgta
    13321 accctgttga caagtgtgaa gcttgtctac gccaatgaag atgatcccaa caatcccaac
    13381 attccaggaa ccatgttgac gcccgacgga gacgacctgc ttgcgacctt caacctgtcc
    13441 tgcttcaacg gcgaaggggg accggccggg ccttatcatc tggagggaac tcgcagttct
    13501 gactgctcca atccggccag gctgagcaac gcgctctggg tggtcaaacc accaaccggt
    13561 tttgcctgcg tctggcaacc gtgggcagcc ccctggagta gtctcaacaa agggcctgat
    13621 cttgatccgg atcccgttgg tgaggcgtac aatcccacga attgggatta ctctttcagc
    13681 cagaacacca atctgggaat ggtgaaggat acgcccgacg gaagtcccac ggccttccac
    13741 ttcttctggg attcacagcc gccggacacg ggcactcaag ccggaagcgg cggtatctac
    13801 caggagatct cagttacgcc gggggtgccg atcgagtact cgttctggtg gaaggggcag
    13861 gcgacgtacc aggcgaacga caacatctgg tacgagctcc tgctgctgga cgggcccttc
    13921 aacctctatt acgccgacgg ttatcaggaa cacacgcggc aacagaacaa tccgtacatg
    13981 gttcgcaaga aggtgggaat ggacagtaat cctttcggct gggagcagat caccgatcag
    14041 acgccgcctg atcaaggtaa ttacggccca cgcccgcaaa ccattacgcc ggtgaacgat
    14101 gttgtgaccg tggtattcaa atgcggccgt gttccgccgg gacggctgga gttcttcgtc
    14161 gacaaggtcg aggtgcgtca gagcggcggt ccgaatcttg tctcaaacgc gaatttcgag
    14221 acgggctctc aggtgtattc gtgcgaaaac gagtgggtgt ttaaggatgc ctgtgagtcc
    14281 gactattggc ggcggtctcc gttgacgccg acagtgtgtc cgaacccgtt cgccgacttt
    14341 gacaacgacg cggacgtcga ccaggccgac tttgcgagct tccagatatg ttacacggga
    14401 ccgacccggc cgcttgtggc aaacgaatgc cgttgtttcg ataacaacaa cgataactac
    14461 atcgatcaga tcgacgtgat cgcctttgaa cggtgcgcga gcgggccggg cgttcctgcc
    14521 gacccgacat gcgatgatta ttgaccccag ggggattcga acccccgttg ccggcttgaa
    14581 aagccggtgt cctaggccac tagacgatgg ggccggtaga ctaccgatcg catcagtata
    14641 gttaaggccg cgcggctggg caaatcgcga cgaccagggt gtggagtttg ccgctcttgc
    14701 ctgctcgtcg ccccagggtt ttacggcgat ccttgcgtgt gaaaggggcc gggaccgaga
    14761 tgccttggcg ataacggcca ccgcagttgg ctgctgtggg tggctgagac tgctgcgctg
    14821 agcaagagga aggtcggcgt gctcagttgc cccgcttgaa accgacgagg cgatgctttg
    14881 tccagtcaat tgcgaatttc ttgccgctgg tatcggtgta ttcaccggtg cccttggcgg
    14941 caaagtcgtt ttcgaccatc cggccggcat tgtcttcatc gcaaatgtac gtctggttgg
    15001 ttctgttttc cgtgttgaat accttgagtt cgcaagccat tgtcagccct ctgtttagga
    15061 gtgcctcccg atgcggtgcg gagccgtttc cgacggttac cggagcggca caatgccgct
    15121 gccgcattgg tttcaggacg cacggtcatc gatctcccac tcggcctttg cgatctgtgg
    15181 accggtgcgg agcggacatt ctacccattc acggcgggtc ggacaatccg ctcatctggt
    15241 cccagccgat cgagaagcct tgtggccgat ccgaacgccc ggcagaggaa tcggcgtcgg
    15301 ttctcgtcaa ccggctcgat gctgatctcc aggcgactgg acgggagaac accctgaacg
    15361 cggtcggccc actcgatcaa aaccgcgcca agcgaaagca tctcatcgaa acccagcgac
    15421 tcaagatcgc ctccgccgcg gagacgatag gcatcgatgt ggtagattcg cggggcgggt
    15481 gaaagaccgg tctcgtactc gttgacgatc acaaacgtgg ggctgttcac ctggcgcgag
    15541 tcagccacgc ccgcgccggc ggcgatgccc ttgaccagcg tcgtcttgcc ggagcccagc
    15601 gggccgataa gggcgacgag gtctcctgcc tggagggccg aaccgagtcg ccggccaagc
    15661 cggcgggttg cttcgacatc ggcgatgttg atcgtccgct gattcatcgg aagtgctcat
    15721 atccccgtgg ttcgatgtag ctgaatcgtc ccaggcgatc gcacaggcat atcgtgggcc
    15781 ggccttcctg tgacggcctg gtcagtcggc cgattcgcgt caggcccagt tcctcggccc
    15841 gcaggccctc gccgccggcg acgaggagct caaaatcttc gccgtcatta agagcgtggt
    15901 ccatcgcgct gatgccgtct tcttgtgaca tggcccgagc ggccgggctg atgacttctt
    15961 ccattgcctc ggtggacagt tccgcgtcgc atcgggacgc cgcgcacatc cgatgcagat
    16021 ccatagacag cccatcggag agatccatca tcgcgtgaag ctcggggtcc agcacaagtc
    16081 ggctggcgag ttccagtcgc ggtgtgaagg tcaggtgctt gccggcggcg cttccgccga
    16141 gcgggccgct gacatagatg ccgtcgccga cacggccgtc gcatcggcgt atcggcccgc
    16201 gcggcgacat cggctcggcc agcatagcta catcaatggc gagcttgccc ggccaactgg
    16261 ttgtgtcgcc tccgacgatg gcacaatcga attctcccgc gatgcacgcc atgccctcgt
    16321 acagcctttt cacatcgtcc atcgacatgc cgtcgtgcag tgcgaggctg acggtggcgg
    16381 cgcgcgggcg gcaagccatg gccgcacagt cgctcagcga gcaggcgatc gcttttctcc
    16441 cgatcagttc gtagatgtgc cggcgggtgt cgaaatggac gccgtccagc agcatgtcgg
    16501 tggtgatctg aaggaggctt ccctccaggc gcaggcaggc catgtcgtcg ccgatgccaa
    16561 tggggacccg cgcggggttg gctgggaatc gccgccgcag ccagtcgatc agtgcatttt
    16621 cgcccctgga catggcatca agcttacaca tcagactttt ttcgggaaag ggaggataga
    16681 ggccctcggc ggctttcgtc gtccggatta cactatcggg ccagggtcgc cacggcgggc
    16741 ccggacggat cttcgaaagg gggcttggtc atggaggctt gtcccctctt gccgggtgcc
    16801 gccgtgtcct gcccgctact gtggcgattg gcggagcggt attggcggag cacggttaat
    16861 cctcggcttg aaagtccgac tcggccatgg gaagggagta cagatagatc accagagccg
    16921 ccggaaagca cagggcggcg accacggccc agccgaggaa ccagatctgc cagagtgatg
    16981 tcaaggtatg ccgctggccg cccaagtaga caaacgcccc actcagtttg aagagcaggg
    17041 taatcagcga ccccaagagc atcaggaagc cgatcaccct gcattgggtc ccgcgaatac
    17101 tcttgccgaa aggaaccggc agcaccgctg caaagagggc ctgtgcggcg aacatggcga
    17161 gctgaagcag tatcgcccag ctcagcgcgg atgccagcgg aagcaggccc atcgtgatga
    17221 gcgggttgcc tcccgagttc attgaggcct gcagcccgta aacctcgccg atgacgccac
    17281 cgacaagcag cggcgaccag tactgccact tggtcatgaa tccggctccc ggttaccgtt
    17341 gataccccga ttaaagcatc agacgctacg ggatgcaagc cacgccgcgg cgccccgaag
    17401 aacagggggc tccacctcgc agcgatgtgt gtcgcgtagc ggggattctt cggtcgctca
    17461 tctatcagtg aaacaaccct ttatcgatcc tataacttcc tttgccatga cttctccgcc
    17521 catggcttgt catattgtca tgggcggcgg agggcgggtt ggtttggcgc tgggggagtg
    17581 gtcgggcttg cggctaccgt acctgccggt ggaggatcgc catcgtcatg ggcgtcgaat
    17641 gcggaaagga ttccgcgggg gattcggccc ggaaaggatt ccgggccgag cgagcgaggg
    17701 tgcggcccgg aaaggattcc gggccgagcg agcgagagtg cggcccggaa aggattccgg
    17761 gccgagcggg tcggtgggtt cgtttggtcg gagagagttc ttggggtgtt ggctcttggc
    17821 ttcggccgga gggggctggt cttcaggttt caaagagcgg gcaagggagc ttccagcaat
    17881 cagcaatcag ctctcagtcg tcagctagac aaggggggga acatgagggg tgctccggaa
    17941 agggcggttg gagcggagcg gcggcggggc tttgggctct gggctgtaag ctcgcggctc
    18001 ttggcggggc cgggggtgtg gggcctgtga cagaagcggc ggcactcctg gcggctgacc
    18061 cggttgtcgc gaacgcaagc cgaaagcaga ccgcttcagc ggtctttccc gaagggcaaa
    18121 caggccggcc gttttacgac tggttcacgg tcggccaatt cctccctcac ggtgtcaccc
    18181 catcgagcct gttttaacgg gcctgtcctt gtcgagtaag cccgttgaaa cgggctgaga
    18241 ccataggaag aacaaccgcg ggcgaccaca accggtcata caacggtcgg cctatcagcc
    18301 cgccttgggc gggaagaccc cctcaagagg gttgattgcc caacctggac cggtcggcat
    18361 gcccgcccag acggtcgcgc actgcaggcg gagcagagat ctcttacaat ctggcgcatt
    18421 gctcggaggt gcgcgagcgt gacaagacgc gaggaacatc aaaccaagca tcggcgggtg
    18481 gtcgagtaca tggaatccca tgccctcgat gcagtggtgc tggctcaacg accgaacttt
    18541 gcctggtaca ccgccggggg gctcaaccat gtgggaacgg ccgacagcac cggcatcgcc
    18601 gcgctgttga tcaccaggga gcggaccatc tgtctcacca acaatatcga agccccgcga
    18661 ctggccgacg aggaactggc cgatttggac atcgaggtca aagcggcatc ctggtttgat
    18721 gagggggccc tgaagcgact ttggttggcg gaggtcggtg gacttcggac agcctgcgac
    18781 gtgccatttc ctgccttgcc gggcggggtg tgctgtctgg acacagactt cgcgacgctt
    18841 cgatgggtca tgacggaggg tgaaatccga cgctaccgca tccttgcccg ggacgtggcg
    18901 gagtgccttg aggttgcctg cggccaagcc gagcctggca tgacggaatt tggtttggcc
    18961 gcccgaatag cccagacggc cctcgccaag ggcattcgca ctccagtcct gctcgtggcg
    19021 gccgatgatc gcataaggca ttatcggcat cccattccga ccggtcgggt ttttgaacgg
    19081 tacggtatgg gtgtcctggg tggtgaacgg cacggtctga ctgtctcagt aacccgcctt
    19141 ttcagctttg gccccattga cccggaactt cgtcgcaaac atgaagcagt ctgccgaatc
    19201 gatgcggcca tgatggctgc cacccgtccg ggcaaggcca tgggcgaggt gttggctgtt
    19261 gctcaggaag tctatgccca gcaaggcttt accgacgaat ggacctttca ccaccagggt
    19321 gggctcaccg gttacctcgg gcgcgaaatc cgcgttgctc ctggctgcct gattgccatt
    19381 caggggcacc aggttttcgc gtggaatccc tcgattgcgg ggaccaagag tgaggatacc
    19441 cttctggtcg gtccagggcg gaatgagatt ctttctctga cgggggggtg gcccacgatg
    19501 ccgtttgccg ttcaaggtca acagtactgt cgctgctgtg tccgggaact gtgagtattc
    19561 ccgttcaaaa acgggaaggt atgaccggcg gcacagaacc gaccttcaag cgcgtttaag
    19621 tatttactta tcaattactt aagatcttgc tgacgagcta tctcctaccg ctcaacttga
    19681 gcttgtttgg caattgggga ctgcgagaac gggtcttgtc cgccccatcg aaaactcaca
    19741 ggatttggtc agccggttgc tgggcgtccc aaggactcca acggccttga caaaaccact
    19801 caactatcat aaagctaccg accgattggt actttttgtg ggaggcgcgc ccatgaacaa
    19861 tacgaacaca gctccggttc aatcgcgcgg cgaggcgacg cgaaccgaca ttcttcaggt
    19921 cgcccgccag ttgttcagca agcatggcta ccacaggact ggtatcgccg acattcagga
    19981 ggcgacgggc ctgaccaaag gtgcgttcta tcatcatttc cattcaaagg aggatctggc
    20041 gctggctgtg ttggagcgga cccgccagga atacgcggaa caactctttg ccccggccat
    20101 gaagcaacac acggctgcgg agcgtctcga ggccctgctc gaccaagcgg ccgagttgaa
    20161 caaccggccc gagtggtgca actgtcagat gttggccacc ctgacggccg agctgacccc
    20221 cgccgacggc cgtcttcgag aggcggttca ggacatctac gacgaccttc aggatcgctg
    20281 ccgcaagctg ctgcgtgaag cccgcgatgc cggccacacc agcgaccagg tcgatccgga
    20341 cacaggggcc cagtggatcg ccaacacgtt gacggggttg agtctggcga agaaactggg
    20401 aatcgcccgc gtgccggcga ccaacctgat tgacgcgatg aaacgcactc tgatcaaacg
    20461 ctcaggcagg gtgaagggac aggcctcggg aaacggtgag tcatccaagt cacaaccgcg
    20521 cacgatacct ggggggagga gcgacgcatg aacggcacgg cggtcttgaa ggcccaactt
    20581 gacatgagtc gccagtggct gatggccctg ctcgcggacg tcgagggggc ggaagtcacc
    20641 gcacccacgc ctaacggcgg caatcatccc ttgtgggctt tgggccacat catccactcc
    20701 gaggccggaa tcgtcaacgg ttttgtcctc ggtcggccaa gccccctggc ccggtgggac
    20761 agactcttcg gtaagggcag ccggccggtt aacgatatca gcgtctatcc ttctctgact
    20821 gagctgcggg cggagtttga cagggttcgc ggtgagaccc tcaacgtgct ctccgagatg
    20881 tctgaatcgg atcttgacag gccgagcaag gctcccacgc acctgtcttc cctgttcgga
    20941 acgatcggcc agtgtttcgc gatcatcggc ctccatgctt gtttccatgc cggccagatt
    21001 gccgatgcac gccgcgccgc gggaaagaaa cccgtgttcg attctcagaa ctgaaccggc
    21061 cttgtgctcg cctgcgccag ggccgtcctg gtttctcaca ctgctgctgc tattacgtct
    21121 ccaccgcctg cacattcctg gtcaaccgct gtatgacgcc tgccttggtg acgaagaaga
    21181 acggcacatt cagactggtc gacgtcgatg ccgtgaacct tggccttcgg ccgactattg
    21241 gcacgcctgg gccggcatgc acatgttgta gccgccggcc gaacagcaga tgctcggaaa
    21301 ctcgcaatcc agatcgttat cgcacagcaa atcgcagaac gtcgcaatct ggcacgtttg
    21361 ctcgccgccg atcggcccac agcatgtcaa ccccggcgtc gtgcaatccg agtcgaaagt
    21421 gcaggcggcg cccattgttc ctggctttgc cttgcatgcg actccggatc cgtcgttgct
    21481 actgctcccg ttgcagccgc tcaagaactc cacctgctcg ggtgtgagga caaaagaaaa
    21541 cccgccggag caggtcattt ccatccgccc cgactcgctg acactgacgg tgtacgagga
    21601 gcccgatgcg gtcgtgatcg tggtttcgct gtcattgccg gtgatcgtcc cgcataatgg
    21661 gttacccttg gccgtcggcc cgtagactct tgtgacaggt ccttgaaaga tggagaactc
    21721 cgactccatc ttggagccgt tctcgaactc aaggacaaag ccggcaccaa aggggttgaa
    21781 aggatcgatc tcgtagaagc atgcgccttg cggcgcatag cacgcataga agtcgcccaa
    21841 gccactggca caggtgttcc agaacgggtt gtcggcgatg ctggctacca cgcaggcagc
    21901 ctcgtcgatc gggccggggt cgacgtcttc ggtggtgatc accggtacct gggtgcagtt
    21961 cgcggagcag tcggccggat atccccctgc ggccagcccc gagccgctgt ggatggagcg
    22021 cgtcgtcacg caataggagt cattccgcat ggccgacggg caactgtccg agatcggtgt
    22081 ccatacctcg attcgcatgt cggtattacg ctcgacgtac atgcagaagt agccgtacat
    22141 atccgtgtag tctgaggaga ttctgccggc cgactccggg atcgcctgga cgaaggcacc
    22201 ggccaccgga ttaccttgcg catccttgac caggccccag atacaggtcg gcacgatggg
    22261 ccgatcgatg ttgcacggcg acagatggcc aatggtggcg atgtagctgt cggtttgagg
    22321 atcgtaaatg gcgtcaccat gttcggattg gacccagaga ctcgtggccg catcgaagat
    22381 ccacacctcc gacgtgtcgg gcttgtctcc cttactggga gccggcacct gcaccgtgat
    22441 ggtctgtcct tgggcgatct gcagcggctg accgttttgc gtgacggtga tgtccaggac
    22501 cccgtaggtg accaggggca gtgtttcacc ggtcagcgtc agaccccgca gctcgccggg
    22561 ataggcagcc aactccgcag ccgtcgcggg atcaaagggc gtgaggcgga catcgacgtt
    22621 gcctgtaacc ggcaggcccg aagcgttcac gaacgcaccg ggggggactg tgatcgacgc
    22681 gtttcgcgca cccgtgacca ccccgccgtc ggaggcgttg agactctgcg gctcggccat
    22741 cggcatcagc gtgacaggca agtaggaact gcccgtctcg ttgacgtcga cgcgaacgcc
    22801 ggcggtcacg tacccttcgc aggagataac gacaatgtgt tcctgtccga caggcaggtc
    22861 gagatagaac atgccctcat tgtccgtcgt agtggtaacg ccaccgcagg caacataggc
    22921 gttggcgatg cgccggcccg ccacgtcttt gacggatcca atgacgttgg cctgcccgtt
    22981 gggcagcggg ccgccatttc catcattcga tgggcaaccg gccagaagac atacgatacc
    23041 aactgcccag cattcccgga caagaagaga tctctttggg agccggatga cgttcttcac
    23101 gtgtgtcatg gcaagtcctc cttctgcgat ggctgggaga tgacatgcgg cctgggactc
    23161 cgatttgccc gttcagcccg gggatgcgac gtcgatgaac tctattgaac caaccgtatg
    23221 ttctctgacg tccttgcccg gccgcgcccc ttggccgcat tctaattata catcacctgc
    23281 gacggcgcca aagctgtttt cagtctttct ccgggttcag gaaatgaggc ttccgtccac
    23341 gcgtgcgaag agggttttcg gtctccacgg ccgttgtggg caccttcaag ccgggtgtgt
    23401 tgttgcaggc tgcattcaac ccgtcgatca gcccgccgca ccggcatggc atgaacccga
    23461 aaacatcatt ggaggcttac ttggccggag cggccgccgt gagaaagagc atcttcgtcg
    23521 gctgagcaac ggcaagaagc tactgcgctt caaagagcaa caactcggag gacatcatgg
    23581 cctccaagcg gacaggcaac ccgtctcgaa cccattccat gccgtctcgt attgccgttt
    23641 cccccgtgct atcaaaggtc gcgcgataag tcttgccgcg gtccaacccg cggggtttga
    23701 atagataggt gtctgtgtcg ctggttccga tccgaacgat ggtagcccat cctttgccgc
    23761 ggtccggagc cgtgtattcc atcgcaaacc agccgcttga ggtgactccc cccgtcgcag
    23821 agaccggggc atggtgatac atcctgcaaa ccggcaggat cggccggatg aacgtcttgt
    23881 agagattcac gtaatgtgca tagcgctcgc gctggcgagg actgagttca cccaggctcg
    23941 gggcaacgcc cagaagaagc cagggcgtcg agagagtgaa gacgttccgc aaataagtat
    24001 ccatgtggcc gctgccattg actccgtggg ccccctcggc caggccgatg acgaagactt
    24061 ccggcggcag ggccagagac tgaccactgt agttctgcag gaccttgggc atggtcaggc
    24121 cgtcggtgag gtagtgttca tggaacctgc ccatggtccc caggtcgttt cgagcgcccc
    24181 cggcggcgca ttgctggagg atcaggttgg ggtacttggc gcggatccgc tcgaagagac
    24241 cgtagaaggc ttcatagtaa cgccagtagt tgtcctccgt gaagccgtga cgtgtcgcgg
    24301 aaggtccttc actcgtgggc agggggttgt agtcaagcct gtagagatcg agcttgtatt
    24361 ggtcaatgag ccgagcgagt tcggattcca cgtaagcggc gacctccggt ttggtgagat
    24421 ccaggatggc gcggggtcct acccagtcag ggtgctcccg ggccaccttg ctggtgccga
    24481 tatcgttgcc cacgttccat gcattgacgc gttcgatctc gacatacagg ccgaagagca
    24541 ggcccttctt gtgagcgtaa tccacgatgg gctcgagacc gtgcggaaag cgagcaggcg
    24601 agggaaccca gtctccggga ttgtcccacc atccggcatc gacgatgaag acgtccgccc
    24661 cgatggccgc tgccaaatcg atgttggcca gtatgttgct ttcgttcatg ccggccgtat
    24721 ccccgaactc gttggccaag taaccctggt cgcctgttgc cgaatactgt accaggcaag
    24781 cccgctctcg tgggtaggca ggcaggacgg agcggcgcag gtgatcgtgc acggcttgca
    24841 cggcgctgtc gaggtcgcct tcgatgtggc ccagatgcac cgccggcgtc tgaacggttt
    24901 catcctgacc gaggattcgc tgggcacacc ctgccgacgg cccgatgccg aagtgcagtg
    24961 cgccgtcttg ctcgcaccgg aaagtcatat gccagttggc ggaccatgcc agatggccga
    25021 cgaagtattc acccctggct tcgttgcgga cgatgaaaaa cggagcgtca aaagcctgcc
    25081 ccagaaggct ttggatcact gtgtctcctg cgggcaacgg cttccactca agccagcctt
    25141 cgaaaccttg gtcctgccgg gtgaagtaac ccaacgtata gtcgtgctcg attccgtggg
    25201 gtggcagcag acgggcattt gtctgcgccc agagcctgcc cgcccaaggc gaaaccgtca
    25261 tcagtgcaag cggtttcctg ccgttattcg ttatctccag ccagcgggtc aggacagggg
    25321 tcccgtcgag catagtgtgt actcttaccg tgacgggagg ggcttcgcgc gaaagctcaa
    25381 cgacgaagtg gcgccggctc gggttggttc cctgtgcttc ggtcgccgag acccatctcc
    25441 aacccttgga gagcgggatc agcggcgcat ccggagcggg gtcgtccctg acctgaagtt
    25501 caaacgcgtc ttcggcccat cgctggtacg ggagattgat ccggccgctt ggagtccaat
    25561 accggccgac ccatcgatct tcgaccagcc cctccacgta aatcgtcgtt ccggaggtgt
    25621 agcgtacctc gtggtcaggt gccggtttca cgttgacgtc gatgaaccct tgtgcggcca
    25681 gcctggaatc caccagaccc atagccgcga ccgcgacaat acccgcgcgc atcagcattg
    25741 acattgcccc ctagcgagtg acttacgcac ctgcggtttc accaccattt tctcgaaggc
    25801 cggtttggcc gaattgcccg ggccgtccgg gcgatggtcg agcttggcga cgccaattac
    25861 tgcagtaatg tactggtggc atgcccgttt atgaagacgg cgttggtaga gcacaggggg
    25921 tgcgtgatcc ggttggtacg gaaacagccc tgccggggct tgtgtcccga ccatcgtgtc
    25981 gcattccgcc tgaaatcgtt ggcgatgacg agggtcgtga ctacgtggtc ttcgtgaggc
    26041 aagaacgagc gtggcggtgc ggtctgacca ttcaaaagaa atctgggaat tgaccttgta
    26101 tgttcaagct tgggccgaga agcgcgcagc ggctctgccc ttccggactc ggctgaatag
    26161 tcgcatccga atagcggtcc ggccagccct ggggggccgc cggtctcacc caatgcgccg
    26221 ttggcggatc agaaccaacc cgcacagacc cagtagagtc agcgccgccg gttcgggagt
    26281 gatgatcacg ttatcgagat agaagttgac gggattctcc gctgccgaat tgctgcccgt
    26341 ccagaactgg aaccaagtat cgccaggctg ccagcccatt tgggcaaagg tcagttcgaa
    26401 gtgccaggtt cgcaagtcgg actggcccac gttctgccag gcgtcaatga gtttgtcgat
    26461 ctgtgtccag ccctgagtgc cgtcgctgtt gccgacagca ttgaactgat accacacgcc
    26521 gctggcgggc gggaaactgg tgccgtcgac catgacgtcg aacgcgatct tggccgtacc
    26581 gctgtttgcc agcgcttgca tcgcaagctg ttgattggcg cttccaccac cgggtcccca
    26641 ggagaactcc ttggtcattt gcatctgcca gccgccgacc gtatgggtct gcatcacagt
    26701 cggcgtgttt ccccagccgg caggggcatt tgcctgccag gacacgttct ggaaaccctc
    26761 agcatcgctg ttgaaggtgt atacaaggtc agcatgagcc gaagcgctga ccatgaacag
    26821 aagggccatt ccaataaaga tcggtttcat cttgttctcc tccaacaaga aggtgtgtgg
    26881 aaaatgacct ctctctcccc gcttgccggt catcgtgcgt cgcactgttt tcggcagcgg
    26941 aaatcctccg aaatgcgtgt caggtagtct accgcatcag tgcccggccg gtcaagcaca
    27001 atcttttccg agaccggcgg attcggtcgg ctccggcagc aactctaaat cgttaccagg
    27061 ggcttggtta tggccgttcg gctaatcgtt tctgctcgtc atttcacagg acgggctcgc
    27121 cggcttgacc gcgttctccg gtcctcgttg ctcagtcagc ccatttcacc accgccaaac
    27181 tgcgcggagc aagcaagagc ctcattccat cgccgcggac ggcgatctcc gcggactgtg
    27241 gcgggatcga cagctccagt ctgggagcct tcatgcctgc cactgtcaga aggacctcgg
    27301 cggggcgatc attgaaactc tcgacggctg cgacgttacc ttcgaacaag tacaaagcga
    27361 ctcgcgtggg gcattgaacc tggactccga acggagcagt catgcggtct cggatccgct
    27421 gcagcgtttc ggccggcatg tccatgagtt tccacgggtc gttcgggacg tccaggatgc
    27481 tgatctcggg cacggcccag tcaaccgagc ccgccagctt ctcggccagt tgcctggtca
    27541 ggagaagcgg cttgtgcgcc gcgaccattg tcgcaatctt ggacgcaaga cccgcgtccg
    27601 ccgaggcttg gagaggaaga aacatcgcag cagcgtcggc gcggatctcg acggccggta
    27661 cgagcggtaa acccagcaag ccgatgtaat cgtagatgta acggttactg cctccggggc
    27721 tgtcgggcgg ctttggggcg gcaatacctc gcggcgtctt gccccgcagg agggccgcga
    27781 gttcgaaaag ctgcgggaac tcgcggcgga acgcctgaac attggcttgc ccctccggac
    27841 tttgcagtga accgtagcaa aacaagagga cttctcgcgc cccggccagg accgtctgcc
    27901 gcccttgctc gagatacaca gccgggatgg tgccgtatgg gtcgaaccat ccaccgccgc
    27961 atttgtcgcc gccgagggtg gcaagccagc gcatgatgaa gtaggcctca tactgcgact
    28021 tacggcccca cttctcgttg tcggggtcgc gggtctcggt gccgacccag atcttgtcaa
    28081 acatgcgggt ttgccgcagt acgtc
//
LOCUS       DAOTPW010000037        27810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1786_length_27810_cov_5.726248, whole genome shotgun sequence.
ACCESSION   DAOTPW010000037 DAOTPW010000000
VERSION     DAOTPW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27810
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_27810_cov_5.726248"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="P5316_05135"
     CDS             <1..349
                     /locus_tag="P5316_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84374.1"
                     /translation="ASIQRLRLWGAIIGGFCFALAVVWLICGVIHRTRTGLPAGVWAR
                     GSLATVASALLLTSLVLWAVLAVNQYFDIRHERAFVEAMIDPVADKLGPNWLDRYFPK
                     PVTERVPTTATTG"
     gene            496..1548
                     /locus_tag="P5316_05140"
     CDS             496..1548
                     /locus_tag="P5316_05140"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRR84375.1"
                     /translation="MADRESKQLSKTSERGRAIQEFVNRFANCEDRELLEDMLVTVCR
                     LASDGTGRGELKILSKAMAELRYAFKVFAPYSEVRKVSMFGSARTPVDHPNYIQADRF
                     ARRMREQHWMVITGAGDGIMRAGHVGAGREGSFGVAIRLPFEQVTNPIIANDAKLINF
                     KYFFTRKLIFLKEASAVALFPGGFGTQDEGFEALTLVQTGKNQLIPIVMIDAPGGTYW
                     SRWRDYVTSELLVKGMISPEDMNLLRLTDDVETAVQEVVRFYRVYHSSRYVRNLLVVR
                     LNEPLSEARIEKLNDEFKHIITAGKIEPHPGPVEGEDGDYPDKPRLAFHFDRKSVGYL
                     RLMIDEINEAGRPPAL"
     gene            1660..2727
                     /locus_tag="P5316_05145"
     CDS             1660..2727
                     /locus_tag="P5316_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRR84376.1"
                     /translation="MFGKRAVPNQFRAAFSPQELATVLARYDLGNIRQIERQLKGSRR
                     SPKAIIISDWGRFLLKRRAHGRDHPLKVAYAHGIQQYLGRQGFPLPRLVPVRKGDDTM
                     VIHNGHIYEVFEYVQGEGYHRLVSETFDAGRVLGMFHRLVRGYDSDWVPSRRGYHDTD
                     IVRECLSSIPMSVGKNDSAFGRETELLTTVNILYDAYETAAEKINEAGFHEWDTQTVH
                     SDWHPGNMMFLNGRVVAVIDYDSLHMLPPITDVANGALQFSIIGGPSDPREWPPELDL
                     ERVRRFLMGYELETPASPEQIQTLPSLMIEALIAEAVAPIAATGSFGRMEGFRFLQMI
                     CRKVGWLQENSERLVAMLLPR"
     gene            2747..3823
                     /locus_tag="P5316_05150"
     CDS             2747..3823
                     /locus_tag="P5316_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRR84377.1"
                     /translation="MVLAIILLSVLAAATDAPVTRPASAPAGLPSALISERHLDRLVT
                     DLNSPRFPIRESATRQLCDLDAAFLPKLAQRYHAATGEEAKHRLRYAMETIFYRRELA
                     GRNGFIGIRLSQQTITDMIDPSDGRRCHGIYILDAIKDMPAANAGVHGGDTIISLNDR
                     PLPAEPTSRQFISLIERTPPGSTIRIRVLRPEKRIRKITVQPPKDEASSTLGLKLSSP
                     PPGLIIGGIRVVKVEKGSAAEAAGLKANDLISAANGVSVSGLLDGLTILTQSLESAGP
                     AAGVQLSVQSCEDVTLEVVVGRRTPEYVTNARDRQEMQDRFARWWRDHGGQWHAPPEP
                     PRRVIPLTAPDTSPLQQERRADIR"
     gene            3894..4910
                     /locus_tag="P5316_05155"
     CDS             3894..4910
                     /locus_tag="P5316_05155"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRR84378.1"
                     /translation="MNAHTDRRSLLKQTLAGAGAALLAGRSAAGADTHPDAAGNRSAD
                     DEPAAAPEFSAYAFGANIWVRIDNRVFTCYRANADQKYPYLYPLTGPATGLSMTDETC
                     EPWPHHRSVFFGCDRVNGANYWQEGNDRGQIVSRGPKLEPLKGRKDASAPGGVTITDI
                     CDWRVPGQSPVIEDRRRYTISAPSRDLRLLDVDITVTAKVDIHISRTNHSLFGIRAAR
                     ELAPVGGGMLINSKGQIGEKATFGQPANWCGYQGRRLGIVESIVVMDHPNNPWAPDCP
                     WFTRDYGNISPTPFLWIDEQKGWDLPVGKSIRMLYRVVVTKGEIEQAAMDRLWEAFAK
                     AEQA"
     gene            complement(5064..5258)
                     /locus_tag="P5316_05160"
     CDS             complement(5064..5258)
                     /locus_tag="P5316_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84379.1"
                     /translation="MEKEKRSYLLPKRLLEVFDQETDRLGYVREKVVAASIAHFMRSS
                     PDDRARMFELLEKVIGGRAK"
     gene            complement(5430..6941)
                     /locus_tag="P5316_05165"
     CDS             complement(5430..6941)
                     /locus_tag="P5316_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR84380.1"
                     /translation="MRPLSRLTVCLLVSGPAALGLLRARADAASGDGSAAGRVNVLLI
                     TADDLNYDSLGVTGCKVPEITPNIDRLASEGMRFEHAHVTIAVCQPSRSVLMTGLYPH
                     RNGAKGFEPIRTDVPTLQESLRAAGYLNGIMAKVGHLEPGEKFCWDTVVPAEQLGNGR
                     DPDLYYRHAKSFFERARNENRAFFLMANSQDPHRPFAGSEQEANRANRGRPAGDAAAV
                     GRTYKPEEVVVPGFLPDQLALFHRWTLPDLPDIRTELAQYFTSVHRCDQTVGRVLEAL
                     KESGSEQNTLVMFLSDNGMSFPYSKTNCYPAGTRTPWIVRWPGQVKPGTVDKDHFISG
                     IDLTPTILEAAGLKPIEGIDGRSFVGLFRGEKDDSRGGVFTYFFQTSGRNDYPMRAFQ
                     TRKYIYIWNPWSDGKTLFRNEPQSGLTFKAMQEAAKKDPAVAARVKLFLYRVPEEFYD
                     VEKDPNALRNLVDDISVKDEITRFRGELFRHMTTTDDPLAERFRKEILPDGPK"
     gene            complement(7679..8896)
                     /gene="amrS"
                     /locus_tag="P5316_05170"
     CDS             complement(7679..8896)
                     /gene="amrS"
                     /locus_tag="P5316_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRR84381.1"
                     /translation="MDNLDNMFRRPNRRDLLKWGTVCGAVCTCGLRPCVAQDVAGTSE
                     LEGPKLKGVIRHPAKHWEKLEDKKVRCVLCPRECEVADVERGYCGVRENQGGRYQTLV
                     YGALCSANLDPIEKKPLFHYLPGTTAFSVATAGCNIECKFCQNWQISQFRPEQVESVA
                     VPPNKLVAACKARSSPTIAYTYSEPVVFYEYVYDSAEQARKAGIGSVIISNGFIQEKP
                     LRELCGQLTGVKIDLKAFTEEFYREHCAGRLAPVLKALEVLKDTGIWFELVILIIPTL
                     NDSPDEIKEMSRWVVRQLGPDVPMHFTRFHPTYRVTNLPQTPVSTLERCRQIALDAGV
                     HYVYAGNVPMHKGENTYCHKCQAELIRRVGFRIASNQVKDGKCPKCGTAIPGVWSQAQ
                     ALAFEPRQQSRPT"
     gene            complement(9109..10134)
                     /locus_tag="P5316_05175"
     CDS             complement(9109..10134)
                     /locus_tag="P5316_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR84382.1"
                     /translation="MSKKGTTRRGFLAKVGAAAAAALGFRRPRAAVAESPSEATRPAV
                     NVKAPARVVIARDEALARGEVGDHVDLLTKLLDAAVQKVTRTDSARRAWSSLFTPGDR
                     IGIKVNTLGLSTQPAVVEAIVAGLRRADVPAGNIIIWDRFDVELLAAGFKLAKSAGAV
                     QCKGTDADSYGSGYQEQIEHSGRIGSCFSRILAEEVTSLICVPVLKDHNLAGVSLGLK
                     NFFGAIHNPNKYHEHNCDPYIVDVVSHRFIRPKWRLTICDATRGQYQGGPTRNPEYAW
                     PFGGLIVGTDIVAVDAVAADLLARQRQAKGLKSLEQEKRPPKHIATAASRGLGTADLA
                     KIERIEL"
     gene            complement(10142..11695)
                     /gene="amrB"
                     /locus_tag="P5316_05180"
     CDS             complement(10142..11695)
                     /gene="amrB"
                     /locus_tag="P5316_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRR84383.1"
                     /translation="MKSEVGLVAGSVVIWAMLAGGTHMANAQDSKTVASREPVCAGTW
                     YPGDAGTLNSLVDKLMDEAKPPAINGKPLAIIAPHAGYRYSAPVAAAAYRCLRGQSYK
                     RVFVMAFSHRRASAYRGVHVPDDLGAYDMPAGAVPLDRETIALLKQNRAFVSIAGIDR
                     DEHSLELQLPFLQRTIKDFKLVPLYVGQMTPEFFALAARAIVQCLDGDTLLVASSDFT
                     HFGPNYGYEPFKDDVPKKLAELADKAAGPLLACDFDGFAEHMKKTDDTICGYGPIMLL
                     LRVLSMKGGAQATRAAFDTSGRMTNDYTSSVTYQSFVFTPRPPLFEEPLRRQLLKLAR
                     ETITAYLSGREPPKVDPTTLPAPLRENGACFVTLQNRGELRGCIGNTEAIDPLYTAVI
                     RNAINACQDPRFTLDPVTAAEVPKLHIEISRLTPMKRVRDTSEVIIGRHGLLISLHGR
                     RGLLLPQVAYERGWTREQFLGEVCRKAGLPMDSWKQPEAELYSFEAEVFGEPETAGQA
                     GQTTAPKPE"
     gene            11863..12288
                     /locus_tag="P5316_05185"
     CDS             11863..12288
                     /locus_tag="P5316_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HRR84384.1"
                     /translation="MKLKISHEEVWVTGITDRPGGLATKLADLARAGANLEFVIARRE
                     SSIPGTGVVFVTPLKGAKQINAAKAAGFAKSKSLHSLRITCANKKGLGATLTRALAKE
                     GINLRGFSGAAIAKQCVFHLAFDKAADARKAAAVLRKMA"
     gene            12321..13322
                     /locus_tag="P5316_05190"
     CDS             12321..13322
                     /locus_tag="P5316_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRR84385.1"
                     /translation="MKAMVLEHNAPIESAPLRLIDTPTPSPQAGEVRIKVRCCAICRT
                     DLHVIEGELPPRKQPIIPGHQIVGVVDALGPRCGRLKIGQRVGAAWLRHTCGQCDFCR
                     TGHENLCGSQLFTGYHADGGYAEYAVVPEAFAYEIPEVFENVQAAPLLCAGIIGYRAL
                     QRSAVPNGGTLALYGFGSSAHVVIQIARHRGCRVCVATRGESHRELAREMGAEWVGED
                     PREMPVKADSAIIFAPAGELVPPALAALKKGGTLALAGIYMTDVPTMQYEEHLFYEKN
                     VCSVTANTREDGRALLAEAARIPIRPHTTTYPLEEANRALQHLKADRIDGSGVLVIG"
     gene            13546..14769
                     /locus_tag="P5316_05195"
     CDS             13546..14769
                     /locus_tag="P5316_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRR84386.1"
                     /translation="MSQLNRRQFIAGAAGLAGALYAQRSPGTPMKSVKIDRIETIPLT
                     YPTAGRFKFFEGPKGEPLGRQTVVVKITADDGTVGWGQSVPTNKWSYETLETVHTTIE
                     RYLTPVLIGLDPFDLTVVHKAMDTIIAPSFSTGQPICKAGIDLALFDLTGKLLKQTAQ
                     ERWKHKGRDKVLLSWTLNPKTLDDIPRLIEEGKQHGYRNFNVKVAPDPKFDVEMCTMV
                     KKLVPDGFLWADANGGYDEATAVEVLPKLAKAGVAVLEQPLKPNRISGYQRLKKIGGL
                     PILMDEGIVSTGELEEFIKLGMLDGVAMKPARCGGITEAQAQIELCLKKGLMWLGSGL
                     TDPDISLAASLLLYGAYDLRFPAALNGPQFLSGLSILKKPLVPTGDLLPVPTGPGLGV
                     EVDEQKLEEHRMKKL"
     gene            14795..15940
                     /locus_tag="P5316_05200"
     CDS             14795..15940
                     /locus_tag="P5316_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRR84387.1"
                     /translation="MLADAPTWSPWFRRCPLWSLLVTALMLPMLVAGCAAPESFRRVQ
                     QPRNGVPLNGRVCLVYSQRYQIDLGGFESLHSFDINKYARIYLQLVTDGLIRPEDVYV
                     PAEIGREDLLRVHTPEYLARLRQPSALARYLENACVALMLPGLADAAILRPFRYATGG
                     TVLAARLAVRYGVAVNLGGGYHHAEPDRGGGFCIYADMPIAIRVLQSEGLIRRALVVD
                     LDAHQGNGTARCLAGDDHVFTFDMHEEDIYPFPKETNDLDISLTAGMENDEYLRMLSD
                     HLPKVFDRARPDIVFLQAGVDVLAGDRLARLRLTPEGIIERDRLVFDEAARRMVPIVM
                     VLGGGYSPQAWQIQYRSIRGVIAKYGATGLPRPASRPATFAEKMYAR"
     gene            complement(16023..16820)
                     /locus_tag="P5316_05205"
     CDS             complement(16023..16820)
                     /locus_tag="P5316_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84388.1"
                     /translation="MAQHAQAFVQSMFVDGDGYYLTGTRGADGTEPNPSPIPADAQVW
                     AVLAGIDEQSRLEQALEWLLNHLVITEVVGEHTYMGVKFSTEGNHIMSEATAGAAMAL
                     WLAVREPEAQSLFDSLEQIQAFAPNSDGNGIVATPFPAGAPTGYGSTYPNSLHVASTA
                     WTSLAWLSRCDSDANPLGPHAGSDSRSDRVPADYDCDCDVDGDDFSLFEACASGPAVL
                     SAVGCEARDFDKDQDVDQSDFGIFQRCYSEVGTIVDPTCAETRAFCE"
     gene            16952..17098
                     /locus_tag="P5316_05210"
     CDS             16952..17098
                     /locus_tag="P5316_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84389.1"
                     /translation="MTVQPCGKCGKLRTGALLPLLVVPELRVWSELVVDAATARDLLR
                     QAPV"
     gene            complement(17382..18617)
                     /locus_tag="P5316_05215"
     CDS             complement(17382..18617)
                     /locus_tag="P5316_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR84390.1"
                     /translation="MMTSRERILAAIDHREPDRLPIDCGAMRSTGIQAVAYGRLKAYL
                     GVKEGQTRVFDVIQQLAEPEDFYLERFRVDAINAGRDFDPNGWKDWVLPDGSPCQIPR
                     HIDFRPDNGDWVAVDAQGKELARMIAGSTYFSQTHYPFNRDDWPDLLDRIGGCMGEVC
                     WGALAEPIYAGGLSDDNLERIAAHVARLRQTTDKAIMIAFGANLFEWSAYLRRMDNAL
                     MDLAAELDKAEALLDKLVETHLAGLDRLLPVLGDNVDLIQLGDDLGMEAGPFFSPELF
                     RKIFKPRYKAIIDRVHTLAPNIRVFLHSCGSIYKLLGDLIEVGVQVINPVQISARDME
                     PARLKKEFGKDVTFWGGGCDTQKVLPRGTPQEVRDHVRRNIDLLAPGGGFVFTQVHNI
                     LSEVPPANIVAMYEEATKS"
     gene            18904..20523
                     /locus_tag="P5316_05220"
     CDS             18904..20523
                     /locus_tag="P5316_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRR84391.1"
                     /translation="MAQLIFNGKHVDIAVGESLLAAIARSGDAAHAVASSCPAAGRCK
                     ECIVQVLAGNEALNDWTEPEAFLRHQSDSETIIHRLACQARVAQTDAVIRVEAFKRKL
                     DIAVNGRPADDAFDPWITCNQGRLFCDDKQFGDCAGPIYGLALDIGTTTVVLHLVELQ
                     TGNTLDMRAFENPQRFGGSDVIHRISYDGHNPGRLHRTIIAYLNREIRALPVDPKGIV
                     AVVVAGNPTMRDLFLGLDVQSIGRSPFMSTTQVDLLAGRRPTTAVWTTGQELGIEIHP
                     QGRVYAMPLISNHVGADTAAALATLPLNDQPFMMIDIGTNTEVVAGCGDRLMAASCAA
                     GPAFEGGRLGCGMAACEGAVTRMRRRPGGWNLSVIGDGPARGICGSGLVDILAELRRT
                     NEIDPLGRFDNGQTHVTVADNPPLRFTRRDAGELAQTKAAIGLGQTVLLRHLRISPRD
                     LRAYYLAGAFANHIDLESGRRIGLLLPVPDERVIRIGNASVEGAKGVLRSRFCRDRIE
                     HLVGRIEHVELEKEPDFFDLFAEMTQLQPIT"
     gene            complement(20583..23336)
                     /locus_tag="P5316_05225"
     CDS             complement(20583..23336)
                     /locus_tag="P5316_05225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRR84392.1"
                     /translation="MRSHDLLWLSAAIGLLPAIGLTMGQGCVTVDVDSDWDGFTDKEE
                     INNDPPTDPFDPLDTPFNVVDSDGDGCSDYGESEFGHCDGNPSTPVARVSVRGSVSVG
                     NTLVVDGDTADASNPTVYNDMVSPGGAQAVPNPCTVGGFLGSISGSDDLRDVYMVQMA
                     GGQRATLLLADPNDNDFDLYLYDESGAQLLDSSEGLGKAEQVTAPANGTYLIEVRGYS
                     VIHDNDQGGLYSLLVGESLTTSVTVQAQRDRLSSLFPHAEGEILVKYAETVKSARAAT
                     QKRIGLEELNDGSNSGGFHRLRLNREAMDAYKSVARARAAGGGMAAEISPTIAAIKAL
                     RRQAGVECAEPNYIRRAFAVPNDEFYGYQWHYRLISLPEAWNITTGSSSVIVAVIDTG
                     VALGHPDLAGQLTTGYDFISSVTTSMDGNGIDADPDDPGDSAGYGISSSFHGTHVAGT
                     IAARTNNTTGVAGVAWNARIMPLRVLGRAGEGTDYDIAQAVRYAARLSNDSGTLPARR
                     ADIINMSLGGPGSSSVLTNAVVAARQAGAIIVTAAGNEATSADNSFPGAINGVVNVSA
                     VDQLRHLASYSNFGSTVAVCAPGGDLNADRDGDGVSDGVLSTIRKDDGRYTYAFYDGT
                     SMACPHVAGVASLMKAVNPDLTPADFDQLLAGTHPGTSVKIVDDLGIAGRDAYFGYGL
                     INAPNAIRAASEIAGSGVSDAPLLRVVPQDISLGSVLTSAEVTVSNVGGGTLIVSSVV
                     SSKSWISVSPSSGGEGSYEITVDRTGLANGVYSGSVTFVSNDGNATVTVRMTVGSITM
                     TGGNVGTVYVLLVDPDTLEAYAQDETDVSENYEFSFDDVYAGNYRLYAGSDLNNDSYI
                     DDEGEAFGGYPVLSKTEVLDIGADLGGLSFSVNYRINVQKAAPLSVPGASVGPRPRLK
                     RLR"
     gene            complement(23682..24491)
                     /locus_tag="P5316_05230"
     CDS             complement(23682..24491)
                     /locus_tag="P5316_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRR84393.1"
                     /translation="MSFFNRYHPITQALIATLFTWFLTAAGAAPVLFFKKVNQKIMDG
                     LLGMAAGVMIAASYWSLLAPAIEMTEGDWKPAVVGFLAGGFFLYIIDKILPHLHLGLE
                     TSEAEGIKTSWQRSVLLVLAITLHNFPEGLAVGVAFGAIGSGVDPEMARSMTLGGVAL
                     AIGIGLQNFPEGMAVSMPLRREGVGRFKAFLMGQASGMVEPVAGVLGAWLVFSMKALL
                     PYGLAFAAGAMIFVVVEELIPESQRDKFTDFATVGTMLGFAIMMSLDVALG"
     gene            complement(24677..24835)
                     /locus_tag="P5316_05235"
     CDS             complement(24677..24835)
                     /locus_tag="P5316_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRR84394.1"
                     /translation="MKRYVCDVCGWVYDPAVGVPEQGIPAGTPFEDLPDDFVCPECGV
                     GKDQFSEA"
     gene            complement(25128..26429)
                     /locus_tag="P5316_05240"
     CDS             complement(25128..26429)
                     /locus_tag="P5316_05240"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HRR84395.1"
                     /translation="MPQLVEQFVLGSWQVLGQMAPYLLFGFVMAGLLSVWISAAWVER
                     HLGGRGFKAVVKGALFGVPLPLCSCSVIPVSVSMHRKGAGRAATTAFLLATPQTGADC
                     IAAMYAVLGPVFAIFSPIMAFLTGLLGGTLVRFFEGREERASGAEATASCVEQGGCGA
                     AFKPPARGSLWSGLRYGLTVLPRDIGAALMAGVLIAGAMTAFIPRGSLEPYIGGGLFS
                     ILLLMAAGVPVYVCATASIPIAAGLIHLGASPGAALAFLIAGPATNAATITTLWKVLG
                     RRSAMLYMVTVAMSAIGFGLLLDAIVTVTGSRVPDLGGHVHGGEAAAIRSHLWAILLL
                     GVLAVSYIVSWRQKITAGAEEAATVTPTDQRVELAVSGMTCEHCANVVRKTLADQPGI
                     SSTEVDVHNGRVIVTGQTLNPESLVEMISSLGYSARLTNAP"
     gene            complement(26561..27112)
                     /gene="dcd"
                     /locus_tag="P5316_05245"
     CDS             complement(26561..27112)
                     /gene="dcd"
                     /locus_tag="P5316_05245"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRR84396.1"
                     /translation="MQDGHLLIEPNPADDQFSPTALDLHIGDDFRQFQPKLYETKGVQ
                     VSFDLDEVQIPDLAPYMEPLPAESNGTVILRPKQFVIATTLERVALPSHGKPAARVEG
                     RSRFARLGLVIHMTAPTIHNSFQGKITLEIMNHGPISLRVTPRVTRLCQLIFERVEEI
                     PGAELVSPFQGQQGPLGSLAPRQ"
     gene            complement(27161..27451)
                     /locus_tag="P5316_05250"
     CDS             complement(27161..27451)
                     /locus_tag="P5316_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84397.1"
                     /translation="MVEELKVRCKVRPGLFKSEYIVTFPVVSSGSRRQIEFVSDADGV
                     MVTAGAAAPPCDASVRVWKVGESDDSYVVLIPASGGSDAMKVSVPAECVLSA"
     gene            27624..>27810
                     /locus_tag="P5316_05255"
     CDS             27624..>27810
                     /locus_tag="P5316_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84398.1"
                     /translation="MSGERIDTVWDRDRLQSPHAQPDKAQRVRRMFDAIAPTYERINT
                     IASAGRDRYWRREMVRLA"
BASE COUNT         5543 a         9001 c         8181 g         5085 t
ORIGIN      
        1 ggcgtccatc cagcgcctgc ggttgtgggg ggcgatcatc gggggattct gcttcgcctt
       61 ggcggtcgtc tggctgatct gcggcgtcat tcatcgaacg agaaccgggc ttcccgcagg
      121 ggtgtgggct cggggcagtc tggccacggt ggcttcggcg ttgctgctca cctcgcttgt
      181 gttgtgggcg gtactggccg tcaatcaata cttcgatatc cggcacgaac gagcgttcgt
      241 cgaggccatg atcgacccgg tcgcagataa actcggcccc aactggctcg atcgatactt
      301 cccaaaaccg gttaccgagc gagtcccgac aacagccaca acaggttgac tcggcgcacc
      361 gcatcgtgag atggacaggc gacgaaaagt cgggcattcc atcccccaag cccgccgggg
      421 aacctgacaa ttcgtgctcc ccgcccgggg caccgatata atgaccggct catacatgcg
      481 gaggaaagct gagtgatggc cgacagggaa tccaaacaac tgtcgaagac ctcggagcgc
      541 gggcgggcaa tccaagagtt tgtcaatcgc ttcgccaact gcgaggaccg ggaactcttg
      601 gaagacatgc ttgtgacagt gtgccgtctt gcctccgacg ggaccggtcg aggggagctg
      661 aagatcttga gcaaggcaat ggccgagctt cggtacgcct tcaaggtctt tgccccctat
      721 tcggaggtgc gcaaggtcag catgttcgga tcggcccgaa cgccggtcga ccatccgaac
      781 tacatccagg ctgaccgatt tgcccgccga atgcgggagc agcactggat ggtcattacc
      841 ggcgccggtg acgggatcat gcgggccggg cacgttggag ccgggcggga aggcagcttt
      901 ggtgtggcca tccgtctgcc gttcgagcag gtaacgaacc cgatcattgc caacgacgcg
      961 aaactcatta acttcaaata cttcttcacc cgcaagctca tcttcctaaa ggaagcctcc
     1021 gctgtggcgc tgtttcccgg cgggttcggc acccaggatg aaggcttcga ggccctcacg
     1081 ctggttcaga cgggcaagaa ccaattgatc ccgatcgtca tgattgacgc gccgggaggg
     1141 acttactggt ctcgctggcg cgactacgtc accagtgagc tgctggtcaa ggggatgatc
     1201 agtccggagg acatgaacct gttgcgactc accgacgacg tcgaaaccgc ggtgcaggag
     1261 gtcgttcggt tctaccgcgt ttatcactca tctcgttatg tacgcaacct gctggtggtg
     1321 cggttgaatg agccgctgag cgaagcgcgg atcgagaaac tgaacgatga gttcaagcac
     1381 atcatcaccg ccggaaagat cgagccgcac cccggacccg tcgaaggcga ggacggcgat
     1441 tatcccgaca agccgcggct cgccttccat ttcgaccgca agagcgtcgg atatctgcgg
     1501 ctgatgattg atgagatcaa cgaagccggc cggcccccgg ccctttgact tgccgccgca
     1561 tctcgttaag atcggagggc aggattgatc caggccgggg gattgcgggg ggctgcgctc
     1621 gccgggcgtt ggggtgagcc ccgcgaggcg gcggttcccg tgttcggaaa gcgtgccgtg
     1681 cccaaccagt ttcgagccgc tttctcgccc caggagcttg ccaccgttct ggcgcgctac
     1741 gatttgggca acatccggca gatcgagcgc caactcaagg gttcgcggcg aagtcccaag
     1801 gcgatcatca tcagtgactg ggggcggttc ctgctcaagc gacgggccca cggacgggat
     1861 cacccgctca aggtcgccta cgcccacggc atccagcagt acctcgggcg gcagggtttt
     1921 ccgctgccca ggctcgtgcc cgtccgcaag ggcgacgaca cgatggtcat ccacaacggt
     1981 catatctacg aggtcttcga atacgtccaa ggcgagggct atcaccgtct cgtttccgag
     2041 acgttcgatg cgggtcgggt gctgggcatg tttcatcggt tggttcgcgg atacgacagc
     2101 gactgggtgc cctcgcgacg cggctaccac gacacggata tcgtccgcga gtgcctgagc
     2161 tcgatcccga tgagcgtcgg caagaacgac agcgcctttg gccgcgagac cgagctgctc
     2221 actaccgtca acattctata cgacgcgtat gaaaccgccg ccgaaaagat caatgaagcc
     2281 ggctttcatg agtgggacac gcaaacggtg cattcggact ggcacccggg caatatgatg
     2341 ttcttgaacg ggcgcgtggt cgcagtcatt gactatgatt cgttgcacat gctgccgccg
     2401 atcaccgacg tggccaacgg ggccctgcaa ttctcgatta tcggcggccc cagcgaccct
     2461 cgcgagtggc ccccggaact ggatctcgag cgcgtccgcc ggttcctgat gggatacgag
     2521 ttggagacgc cggcctcgcc ggagcagatc cagacgctgc catcgttgat gatcgaagcc
     2581 ctcatcgccg aggcggtggc tccgatcgcc gcgaccggct ctttcggccg gatggaaggt
     2641 ttccgctttc tgcagatgat ttgccgaaag gtcggttggc tccaggagaa cagcgagcgg
     2701 ctggttgcga tgctgctgcc gcgatgatcc aggagcgggc ggtattatgg tcctcgcaat
     2761 aatccttctc tcggttcttg ctgcggcgac cgacgccccg gtgacccgtc ctgcctccgc
     2821 ccctgccggc ctgccgtctg cgctcatcag cgaacgtcat ttggatcggc tcgtcaccga
     2881 cttgaacagc ccacgattcc ccatccgtga gagtgcgacg cgccaattgt gcgatctcga
     2941 cgccgccttt ctccccaagc tcgctcaacg ttaccacgca gccaccggtg aggaggccaa
     3001 gcatcggcta cgatatgcca tggaaacgat tttctaccgg cgggagctgg ccggacgcaa
     3061 cggtttcatc ggcatccgat tgtcgcagca gacgatcacg gacatgatcg atccgtcaga
     3121 cggccgcagg tgccacggca tctacattct cgatgccatc aaggatatgc cggccgccaa
     3181 cgccggcgtc cacggcggtg acaccatcat cagcctcaac gatcggcctt taccggcaga
     3241 accaacttcc cggcagttca tttccctgat cgaacggacg ccccccggat cgacgatccg
     3301 gattcgagtt ttgcggccgg agaagcgaat tcgaaagatc acagttcagc cgccaaagga
     3361 tgaagcgtcg tccactctgg gcctcaagct ctcctcacca ccgccgggtc tgatcatcgg
     3421 cggaatccgc gttgtaaagg tggagaaggg ttctgcggct gaggccgcgg gtctgaaagc
     3481 caacgatctg atttcggctg caaacggcgt ttcggtctca ggattgctgg acggactcac
     3541 gattctcacc caatcgctgg aatcggccgg tccggccgca ggcgtgcaat tatccgttca
     3601 gtcgtgcgag gacgtcacac tggaagtcgt cgtcggacgc cgcacgccgg aatacgttac
     3661 caacgcccgt gacagacagg aaatgcaaga ccgcttcgcc cgctggtggc gcgatcacgg
     3721 cggccaatgg cacgctccgc cggaaccgcc ccgacgtgta attcccctta ccgccccgga
     3781 cacaagcccg ttgcagcagg agcgaagggc ggatatccgt tgaccacatt gacaaccacg
     3841 tttctcgtgc ccaaactgac cgccgcagct ttacaacttt atggagacat accatgaacg
     3901 cccataccga tcggcgcagt cttctcaagc agacccttgc cggagcgggc gccgccttgc
     3961 tggccggtcg gtccgccgct ggggcagaca cgcatcccga cgcggccgga aaccgatccg
     4021 ccgacgacga gcctgccgcg gcccccgagt tctccgccta cgccttcgga gcaaacatct
     4081 gggtgcggat cgacaaccgc gttttcacct gctaccgggc caacgccgac cagaagtacc
     4141 cgtatttgta tccgctgaca ggccccgcca ccggattgtc catgaccgac gaaacctgcg
     4201 aaccgtggcc tcatcatcgc tccgtcttct tcggctgcga tcgcgtgaat ggggcgaatt
     4261 actggcagga gggcaacgat cgcggccaga tcgtctcgcg cgggcccaag ctggaaccgc
     4321 tcaaagggcg gaaagacgcg agtgcccctg gcggggtgac catcaccgac atttgtgact
     4381 ggcgcgtgcc cgggcaatca ccggtcatcg aggatcggcg acgatacacg atcagcgccc
     4441 catcacgaga cctgagactc ctggacgttg acatcaccgt cacggcgaag gtggacattc
     4501 atatcagcag gacaaaccat tcgcttttcg ggatccgcgc ggcccgggaa ttggcccccg
     4561 tgggcggcgg gatgctgatc aacagcaagg ggcagatcgg cgaaaaggcc actttcgggc
     4621 aaccggccaa ctggtgcggc tatcagggcc ggcgtctagg aatcgtggaa tcaatcgtgg
     4681 tcatggatca cccgaacaat ccctgggccc cggattgccc gtggttcacg cgggattacg
     4741 gcaatatctc cccgaccccg ttcctgtgga tcgacgagca gaagggttgg gacctgccgg
     4801 tcggcaagtc catccgaatg ctttaccgtg tggtcgtcac gaagggcgag atcgagcagg
     4861 cggccatgga ccgactctgg gaggccttcg cgaaggcgga acaagcgtga acgcgtcgcg
     4921 accggccatg cgtgtcaacc ggtgtcggga gaactaaaag gttcctccga gcggtgccac
     4981 gagtgccggc aaatacgttc actgcggtcg gcgcgagcgg catcgactgc ggcgacctcg
     5041 gatcgcgcca tgcagcctgg aagctacttc gctctgccgc ctatgacctt ctcaagcaac
     5101 tcgaacattc tcgcgcgatc atcgggggaa ctgcgcatga aatgagcgat tgatgccgcg
     5161 accactttct ctcggacata gcccagcctg tcggtttcct ggtcaaagac ctccaggagt
     5221 ctcttcggca acagatatga tcgtttttcc ttttccatgc tcaactcctt tcgcgacaag
     5281 cgactgcaac ggccgaacag caccggaaaa agagtactcc gcggggcagt gcgaatcaag
     5341 aaccgcgcgg tcggcgctcg ccggaacaaa aggtgctcgc cgccggcagc attgaccgga
     5401 gctggaggca cggtgaaaag cacaccgctt cacttggggc cgtctggcag tatctccttc
     5461 ctgaatcgct ccgcgagcgg atcatcggtc gtggtcatgt gtctgaagag ctcgccccgg
     5521 aaacgggtta tctcgtcctt cacggagata tcatcgacca ggtttctcag cgcgttgggg
     5581 tctttttcga cgtcgtagaa ttcctcgggt acgcggtaca ggaagagctt gacccttgcg
     5641 gcgacggccg gatccttctt tgcggcttct tgcatggcct tgaaggtcag accgctctga
     5701 ggctcgttcc ggaacaacgt cttgccgtcg gaccaggggt tccagatgta gatgtacttg
     5761 cgcgtctgga aggcacgcat cggatagtcg tttcttcccg aggtctggaa gaagtatgtg
     5821 aacaccccgc cgcgagaatc atccttctca ccacggaaca accccacaaa cgagcgcccg
     5881 tcgatgccct caatcggctt gaggccggct gcctccagga tcgtcggcgt caagtcgata
     5941 cccgagatga aatggtcctt atcgacggtc ccgggcttga cctgtccggg ccagcgcacg
     6001 atccacggtg tccgcgtgcc ggccggataa cagttggtct tcgagtacgg gaaggacatg
     6061 ccgttgtcgc tgagaaacat caccagggta ttctgctccg agccgctttc cttgagggct
     6121 tcaagaaccc gccctacggt ctggtcgcag cgatgcaccg aggtgaaata ctgggcgagt
     6181 tcggtgcgga tgtcgggcaa atcgggaagg gtccatctgt ggaaaagcgc cagctggtcg
     6241 ggcaggaagc cggggacaac aacttcctcg ggtttgtacg tgcgacctac agccgcagca
     6301 tcacccgccg gccgtccgcg attcgcgcgg ttcgcctcct gttcgctgcc ggcaaagggc
     6361 cggtggggat cctgcgagtt ggccatgagg aaaaacgccc gattctcgtt gcgggctctc
     6421 tcgaaaaagc tcttggcgtg tcggtaatac agatccgggt cgcgaccgtt gcccagttgc
     6481 tcggcgggca caaccgtgtc ccagcagaac ttctcgccgg gttccagatg gccgactttg
     6541 gccatgatgc cgttgagata accggccgct cgcaacgact cctgcaacgt cggcacatca
     6601 gtgcggatcg gctcgaagcc ctttgctcca ttgcggtggg ggtagaggcc tgtcatcagc
     6661 accgagcgag aaggctggca cacggcgatg gtgacgtggg cgtgctcgaa ccgcatcccc
     6721 tcggaggcca gtcgatcgat gttcggcgta atctccggca ctttgcagcc ggtgaccccc
     6781 aaggaatcgt aattcaggtc gtcggcggta atcagtagca cgttgacccg gccggcggcg
     6841 gagccatcgc cactggcagc gtcggcccga gcccgcaaca agcccaatgc cgccggtccc
     6901 gacaccaaca ggcacaccgt caatcgagat aacggcctca taggaaatga aacctcacaa
     6961 gaaggtcagg cacgagcgtg tcgcgcggcg cctcggcgcc ggacgggacc caccggctcg
     7021 ctcaaggccc tcgatgttcg cttgtcccat catatttgtc cagcggccgt ggcgttcgaa
     7081 cgctactacg gcgatagtgc cgactacccc atcacgagac cagctatagc cagtcatgag
     7141 caaagcaaaa ggacttcgcg aattcgatct ttgacaagtg aggattcctt aaagctgttt
     7201 gggcggcgtt tttcaggccg gggacctgga aagatagtcc ccgcgatcgg tcatgatccg
     7261 ccaggctacg cgggtcagct cgacggctac ggcggtgact gcgaccaacc gccatcgaag
     7321 cccgtctgat cgcagaggcg acgaaggtgt gcgccgagat cagccggccg gcttgcggcg
     7381 agagatctcg tatacgctcg cgacccccag gttcactggc aggaggccgg tgcccacgat
     7441 ggaatacacc gtcaaggaca tcccctcgct gattcaggca gtgcaacgcg acaagcaagg
     7501 gctcgatgaa gaactgtggt ttcacgaaac gacgaaaacc gcgtatgatc tcgtgccatc
     7561 cgctcaccgt cggtatggca agagcaatga acgagttccc cggcaggtct cgccgccgtt
     7621 ggcgggtccg accatgctct acgcttctcg gacacggtct gccggttcaa caatccattt
     7681 acgtcggtct gctctgctgt ctcggctcga acgccaaggc ctgtgcctgt gaccagacgc
     7741 ccggaatggc cgtgccgcac ttggggcact tgccgtcctt gacctggttg gaggcaattc
     7801 ggaaacccac tcggcggatc agctcggcct ggcacttgtg gcagtaggta ttctcgcctt
     7861 tgtgcatggg cacgttcccc gcgtagacgt aatggacgcc cgcgtcgagg gcgatctggc
     7921 ggcagcgctc cagcgtcgaa accggcgtct gcggcaaatt ggtcacgcgg taggtcgggt
     7981 gaaatcgcgt gaagtgcatc ggcacgtccg gaccaagctg ccggacgacc cagcggctca
     8041 tctccttgat ttcgtccggc gagtcgttga gtgttgggat gatcagaatg accagctcaa
     8101 accagatgcc cgtgtccttg agcacttcga gcgccttgag gaccggggcc agccgcccgg
     8161 cacagtgctc gcggtagaac tcctcggtaa atgccttcag gtcgatcttc acgccggtca
     8221 gttggccaca gagctcacga agaggtttct cctggatgaa cccgttggag atgatcacgc
     8281 tgccgatccc ggccttgcgg gcttgttccg ccgagtcgta aacatattca tagaacacca
     8341 ccggctccga gtaggtgtac gcgatggtcg ggctggaccg ggccttgcac gcggcgacaa
     8401 gcttattcgg cggtacggcg acgctctcga cctgctcggg gcggaactgg ctgatctgcc
     8461 agttctggca gaacttgcac tcgatgttgc agccggccgt cgccaccgag aatgccgtcg
     8521 tgccgggcag gtaatgaaac aacggtttct tctcgatagg gtcgaggttc gccgaacaca
     8581 gggccccgta aaccagcgtc tgataccgtc caccctggtt ttcgcgaaca ccgcagtaac
     8641 cccgctcgac gtcggctacc tcgcactcgc gcgggcagag cacgcacctg accttcttgt
     8701 cctccagctt ctcccagtgc ttggccggat gccgaatgac gcccttgagc ttcgggccct
     8761 ccagctcgct cgttccggcg acgtcctgag cgacgcaagg ccgcagtccg caggtgcaca
     8821 ccgccccgca gacggtaccc catttcagga ggtcacgccg gttcggacgc cggaacatgt
     8881 tgtcgaggtt gtccatgtca gcgctcgatc cgttcgattt ccgccaggtc ggcggtgccc
     8941 aattgtgctg gccccaggat tctttcacgc tggccccagg attctttcac gctggcccca
     9001 gaatcccttc tgggcccccg gtcagaggat tcccatcctc attatcaccg aatgcggaat
     9061 ggattccgca cactcacacg gcccggaagg gattccgggc cgagcggatc agagctcgat
     9121 ccgctcgatc ttcgccaggt cggcggtgcc caatccgcgg ctggcggccg tcgcaatgtg
     9181 cttgggcggg cgcttttcct gctccagcga cttaagcccc ttggcctgcc gctgcctggc
     9241 caacaggtct gccgccacgg catcaaccgc cacaatatca gttccaacaa tcagcccgcc
     9301 gaatggccag gcgtactccg ggttgcgcgt cggaccgccc tgatactgcc cccgcgtcgc
     9361 gtcgcaaatc gtgagcctcc acttcggccg gatgaaccgg tgcgagacga cgtccacaat
     9421 gtacggatca caattgtgtt catggtactt gttcgggttg tgaatcgcgc cgaagaagtt
     9481 cttcaggccc agcgaaacgc ccgccagatt gtggtccttg agcaccggca cacagatcag
     9541 cgaggtcacc tcctccgcca ggatccgcga gaagcacgaa ccaatccgcc cgctgtgctc
     9601 gatctgttcc tggtaaccgc tgccgtagct gtcggcgtcg gtacccttgc actgcaccgc
     9661 gccggcggac ttggccagct tgaatccggc agcgagcagt tccacatcga accggtccca
     9721 aatgatgata ttcccggccg gaacgtccgc tcggcgcaac ccggcgacaa tggcctccac
     9781 caccgccggc tgagtggata agcccagcgt gttgaccttg atgccgatcc ggtcaccggg
     9841 cgtaaacagg ctcgaccacg cccgacgggc cgaatccgtg cgggtgacct tctgcacggc
     9901 cgcgtcaagc agcttcgtca gcagatcaac gtgatcaccg acctcgcctc tggccagcgc
     9961 ctcatcacgc gcgatgacca cacgagccgg ggcctttacg tttacggcag gacgcgttgc
    10021 ttcgcttggt gattccgcaa ccgcggcccg cggtcggcga aaacccagcg ccgcagcggc
    10081 tgccgccccg acctttgcta gaaaacccct tctggtcgta cctttcttgg acattgttgc
    10141 cctactccgg ttttggggcg gtggtttgcc cggcttgtcc cgccgtctca ggttccccga
    10201 acacttctgc ctcgaatgag tacaactccg cctcaggctg cttccatgag tccatcggca
    10261 agccggcttt gcggcagact tcgcccagaa actgctctcg cgtccaacct cgttcgtaag
    10321 ccacctgcgg cagcagcaac ccgcgccgcc cgtgcagcga aatcagcagg ccgtggcggc
    10381 cgatgatgac ctcgctggtg tcccggaccc gcttcatcgg cgtcaggcga ctgatttcga
    10441 tgtgcagttt ggggacctcg gccgccgtga ccgggtcaag agtgaaccgc ggatcctgac
    10501 aggcattgat ggcgttgcgg atcacggcgg tatagagcgg atcgatcgcc tccgtgttgc
    10561 cgatgcaacc acgcagttcg ccccggtttt gaagggtcac gaaacatgcc ccgttctcgc
    10621 gcagcggtgc cggcagcgtc gtcggatcca ccttgggcgg ctcccggccg ctcagatagg
    10681 cggtgatcgt ctcgcgagcc agcttgagca actgccgccg aagaggctcc tcgaagagcg
    10741 gcggcctcgg cgtaaaaaca aacgactggt atgtcacgct gctggtatag tcattggtca
    10801 tgcggcctga ggtgtcgaac gccgcacgcg tcgcctgggc cccgcccttc atcgaaagaa
    10861 cccgcaacag cagcatgatc ggcccgtaac cgcagatggt gtcgtccgtc ttcttcatgt
    10921 gctcggcgaa gccgtcgaag tcgcaggcta gcaacggtcc ggccgccttg tcggccagtt
    10981 cggccagctt cttgggcacg tcatccttga aaggctcgta accgtagttc ggaccgaagt
    11041 gggtaaagtc gctgctggct accagcaggg tatcgccgtc aaggcactgt acgattgccc
    11101 gtgccgccag agcgaaaaac tcaggcgtca tctggccgac atacagcgga accagcttga
    11161 aatccttgat cgtgcgctgc aggaaaggca attgtagctc cagagaatgc tcgtcgcggt
    11221 ctataccggc gatcgacacg aatgcacggt tctgcttgag cagcgcgatg gtctcccgat
    11281 cgaggggcac cgcgccggcg ggcatgtcgt aagcgcccag gtcatcgggg acatgaacgc
    11341 cgcggtacgc ggatgccctc ctgtggctga aggccatgac aaagacccgc ttataggatt
    11401 gccctcgaag gcaacggtat gccgccgccg caaccggagc gctgtatcga tacccggcgt
    11461 gcggggcaat gatcgctagc ggcttgccgt tgatggccgg cggcttggct tcatccatca
    11521 gcttgtcgac caggctgttc agggtgccgg catcccccgg ataccatgtc cccgcgcaaa
    11581 ccggctcccg actagcgact gtcttgctgt cttgggcatt tgccatgtgc gtgcctcctg
    11641 ccaacatggc ccagatcaca actgaacctg caaccagacc cacttcagac ttcatggcgt
    11701 gacctccaca cgaggtgtgc cataagtata ccacgtttcg gcgcggcaag agacgtcaac
    11761 tcggcaaagc ggggaaatca aacatagaaa aaggccgatt gacagcaacc gccatctgcg
    11821 gttagatctc tgcacgacaa gtctcttatt ggaggacaca tcatgaaact gaagatctct
    11881 catgaggagg tatgggtcac gggcataacg gaccggcccg gcggcctggc caccaagctg
    11941 gcggacctgg cccgggcggg tgcgaatctg gaattcgtca tcgcccggcg agagtcgagt
    12001 atccccggca ccggtgtcgt ctttgtgacc ccgctcaagg gcgccaagca gatcaacgcc
    12061 gccaaggcgg ccggcttcgc caagagtaag agcttgcatt cgttgcgcat tacctgtgcg
    12121 aacaagaaag ggctcggagc gacgctcacc agggcgcttg ccaaagaagg aatcaatctg
    12181 cggggcttct cgggcgccgc catcgccaag cagtgcgttt ttcacttggc tttcgacaag
    12241 gccgctgacg ctcgaaaagc agcggcagtt ctcagaaaaa tggcctgacg cccctcatcc
    12301 cgcaagagga atccgtcccc atgaaggcca tggtgctcga acacaatgcg ccgatcgaat
    12361 ccgcacctct gcgcctgatc gatacgccga ccccgtcgcc tcaggccggc gaggtccgca
    12421 tcaaagtgcg ttgctgtgcc atctgcagga cggacctgca cgtgatcgag ggagagctgc
    12481 cgccgcgaaa gcagccaatc attccgggcc accagattgt cggcgtcgtg gacgctctgg
    12541 gcccccggtg cgggcgcctc aagatcgggc aacgagtcgg cgcagcctgg ttgcggcaca
    12601 cctgcgggca atgcgacttc tgccgaacag gtcatgagaa cctctgcggg tcgcagcttt
    12661 tcaccggcta tcacgccgac ggcggctatg ccgagtatgc ggtcgttccc gaagcgttcg
    12721 cctatgagat tcccgaagtc ttcgagaacg ttcaggccgc cccgctctta tgtgcgggga
    12781 tcatcggcta ccgggcgttg cagcggtcgg ccgtgccgaa tggcggcacg ctcgccttgt
    12841 acggattcgg ttcgtcggct cacgtggtca tccagatcgc tcgtcatcgc ggctgccgcg
    12901 tgtgcgtggc gacccgcggt gagagccatc gcgaactggc ccgggaaatg ggagctgagt
    12961 gggtcggcga ggatccgcgc gagatgccgg tcaaggccga cagcgcgatc atctttgctc
    13021 cggccggcga gttggtgccc ccggcgctgg ccgcactcaa gaagggcgga acgctggcgc
    13081 tggcaggcat ctatatgacc gatgtgccca ccatgcagta cgaggagcac ctgttttacg
    13141 agaaaaacgt ctgctcggtg accgccaaca cccgcgaaga cggccgggcg ctattggccg
    13201 aggccgcaag gatcccgatt cgcccgcaca ctacgactta ccctcttgag gaggccaacc
    13261 gcgctctgca gcacctcaag gccgaccgca tcgacggctc aggggtactg gtgatcggct
    13321 gattttcctc gcgtcttgac gagccggcgg cttcatgccg gcggcactgc gggtacttgt
    13381 gcaggtctcc gcgataagcg gccgtggctc gccaagtaag tgtctcctcg tttcctgcaa
    13441 gtggagaacg atgaccgacc aacggtctgc cgcgtggaag gctctctgat tacctgatag
    13501 acttgaactt aatcaggaat tcgccgtgaa gcaggaggtc cgcccgtgtc tcaactaaac
    13561 cgtcgccaat tcatcgccgg ggctgcgggg ttggccggcg cgctgtatgc acaacgatca
    13621 ccggggacac cgatgaaatc cgtgaagatc gatcgaatcg agaccattcc tttgacctac
    13681 ccgacggccg ggcggttcaa attcttcgaa gggcccaagg gcgagccgct cgggcgacag
    13741 acggtggtgg tcaaaatcac cgccgacgac ggcaccgtcg gttggggaca aagcgtgccg
    13801 accaacaaat ggagctacga aacgcttgaa accgtccata ccaccatcga gcggtacctg
    13861 acccccgtgc tgatcgggct ggacccgttc gacctgacgg tcgtccacaa ggctatggac
    13921 accatcatcg ctccgtcgtt ttccaccggc cagccgatct gcaaggcggg aatcgacctg
    13981 gcgctcttcg acctgacggg caagctgctc aagcagacgg cgcaggagcg atggaaacac
    14041 aagggccgcg ataaggtgtt gctcagttgg acgctcaacc ccaagactct tgatgatatc
    14101 ccccggttga tcgaggaggg caagcagcac ggctaccgca atttcaacgt gaaggtcgcc
    14161 cccgatccga aattcgacgt cgagatgtgt accatggtca agaaactcgt gcccgacggt
    14221 tttctgtggg ccgatgccaa cggcgggtac gacgaggcca cggccgtcga ggtattgccc
    14281 aaactggcca aggccggtgt ggcggtcctg gagcagcccc tcaagcccaa ccggatctcg
    14341 ggctaccagc ggctcaagaa gatcggcggg ctgcccatcc tgatggatga ggggatcgta
    14401 agcaccggcg aactggagga gttcatcaag ctgggcatgc tcgatggtgt ggcgatgaag
    14461 ccggcccgct gcgggggcat caccgaggcc caggcccaga ttgagctttg cctcaagaaa
    14521 ggactgatgt ggctgggcag cggcctgacc gacccggaca tctcgctcgc ggccagcctg
    14581 ctgctctacg gggcatacga tctgcgtttc ccggccgccc tcaacggccc ccagttcctg
    14641 agcggtctga gcatcctgaa aaagcccctg gtcccaaccg gcgacctgct gcccgtcccc
    14701 accggccccg gtctgggcgt ggaagttgac gagcagaagc tggaagagca tcggatgaag
    14761 aagctctgac gagaataggg agacgactcg actcatgctg gcggatgctc ctacttggtc
    14821 gccttggttt cgccgatgcc ccctttggtc gttactggtc accgcgctca tgctgcccat
    14881 gctggtggcc ggctgcgccg ctccggagtc gttccgtaga gtccagcagc cacggaacgg
    14941 cgtgcctttg aacggacgcg tgtgcctggt ctactcgcaa cgataccaga ttgacctcgg
    15001 tggcttcgag agcctccact cgttcgacat caacaagtac gccaggatct acctgcagct
    15061 cgttaccgac gggctgatcc gacctgagga cgtctacgtg cccgcggaga tcggccgcga
    15121 ggatctcttg cgcgtccaca cgcccgagta cctggcccgg cttcggcaac cctcggccct
    15181 ggcccggtat ctcgaaaatg cctgcgtggc gctgatgctg ccggggctgg ccgatgctgc
    15241 catcctgcgc cccttccgat atgcgacggg cggcacggtg ctggcggcaa ggctggccgt
    15301 gcggtacgga gtcgccgtca acctcggcgg cggctatcac cacgccgagc ccgatcgcgg
    15361 cggcggattc tgcatctacg ccgacatgcc catcgcgatt cgagtcttgc agtccgaagg
    15421 gctcatccgc cgcgccctcg tggtcgatct ggacgctcat cagggcaacg gcacagcccg
    15481 ctgcttggcc ggcgacgatc acgtgttcac gttcgatatg cacgaagagg acatctaccc
    15541 gttcccgaaa gagaccaacg atctcgatat ctcgctgacg gccgggatgg aaaacgatga
    15601 gtacctgaga atgttatccg accatctgcc aaaggtgttt gatcgcgcca ggcccgacat
    15661 cgtgttcctg caagccggcg ttgacgtcct ggcaggcgac cggctcgcac ggctgcgact
    15721 gacacccgag ggaatcatcg aacgcgaccg gctggtgttc gatgaggccg cgcggcgaat
    15781 ggtcccgatc gtcatggtgc tcggcggcgg ctacagcccg caggcctggc agatccaata
    15841 ccgcagtatc cgaggcgtga tcgcaaagta cggtgccacc ggcttgcccc gacccgcttc
    15901 acgccccgcc acgttcgcgg aaaagatgta cgcaagatag tcaagtaccg cgcaggttgt
    15961 gtcttcgatc ctgcaccccc caaggtccga ccgaccgcgg gctcccgagc cttctgcgac
    16021 tttcattcgc aaaacgctct ggtttccgcg caggtcggat cgacgatggt gccaacctcg
    16081 gaatagcacc gctggaatat gccgaagtcg gactgatcca catcctgatc tttgtcgaaa
    16141 tcccttgcct cacaaccgac cgccgacaac accgcgggac ccgacgcaca cgcctcaaag
    16201 agactgaaat cgtccccatc cacgtcgcag tcgcagtcgt aatcggccgg cacccgatcc
    16261 gaccgagaat ccgagccggc atgtggcccc agcggattgg catccgaatc gcaccgagaa
    16321 agccacgcca ggctcgtcca ggccgtgctc gccacgtgca gggaattggg atacgtcgaa
    16381 ccataccccg tcggagcacc agccgggaag ggcgtcgcca caatgccgtt tccatcgctg
    16441 ttcggagcga atgcctggat ctgctcaagg gaatcaaaga gactttgtgc ctccggctct
    16501 cgcacggcca accatagagc catcgcggcc ccggccgtcg cctcactcat gatatggttg
    16561 ccttccgtcg agaacttcac gcccatatag gtatgctcgc cgaccacctc ggtaataaca
    16621 agatgattca acagccactc cagagcctgc tccagtctcg actgctcatc gatccccgcc
    16681 agcacagccc acacctgggc gtcggccgga atcggtgacg gattgggttc agtaccatcc
    16741 gctccccgcg tcccggtcag gtaatacccg tctccgtcca cgaacatgct ttggacgaat
    16801 gcctgggcat gctgagccat actgttccat ttcgcgtgac cggtcaggtc atacaatacg
    16861 cgacccagag caaagacatc gatattgtgc tcggtggatc tccaggtaac cgcttcataa
    16921 ttccacccga gcagacctcc gggccgtcgt cttgaccgtc cagccgtgcg gcaagtgcgg
    16981 caagctgcgc acgggggccc tccttccgct gctcgtcgtc cccgaactgc gggtatggag
    17041 tgaactggtt gttgacgccg ccaccgcccg cgatctcctg cggcaggcgc cggtgtgagc
    17101 aaggccgcga atgggaaccc aataccggct cgggtgccgc ggtcgcgctg ccgttgaaca
    17161 gccgctaccg cgtcccccgc cgtcggccgc ccgaccgggg atgcttctgc cccggcgacg
    17221 tttcgcccca atgatgcgac accaccggag atcagtcaaa aagcctccgg acaggcccgg
    17281 tcacaatagc gagtggcatg acaaccgacc ataatgcggc acaggccgcg gatggattac
    17341 acgtccaagt cccccaattc gcaactcggc attctctcaa ctcaactctt cgtcgcctct
    17401 tcatacatgg caacgatgtt ggccggcggg acctcggaga gaatgttgtg cacctgggtg
    17461 aagacgaatc cgccgcccgg cgcgagaagg tcaatgttgc gacggacgtg gtctcggacc
    17521 tcttgcggtg tgccgcgcgg caggaccttc tgcgtgtcgc atccgccgcc ccagaacgtg
    17581 acgtccttgc cgaactcttt cttgagtcgg gcgggctcca tgtcgcgggc cgaaatctga
    17641 accgggttga tcacctggac gccaacctcg atcagatcgc ctaagagctt gtagatcgaa
    17701 ccgcacgagt gcaggaaaac ccggatgttg ggggcaagcg tgtggacccg gtcgatgatg
    17761 gccttgtacc gcggcttgaa gatcttgcga aacagctccg gcgagaagaa cggaccggcc
    17821 tccataccca gatcgtcgcc caactggatc agatcaacat tgtctccaag caccggcaac
    17881 aggcggtcca agcccgccag atgcgtctcc accagcttgt cgagcaacgc ctcggccttg
    17941 tccagctcgg ccgccagatc catcagcgcg ttatccatgc ggcgaagata agccgaccat
    18001 tcaaaaaggt tggcgccgaa ggcgatcatg atcgccttgt ccgtcgtctg ccgcaggcgg
    18061 gcgacatggg cggcgatgcg ctcgaggttg tcgtcgctca gcccgccggc gtaaatcggc
    18121 tccgccaaag cgccccagca gacttccccc atgcacccac caatccggtc gagtaaatcc
    18181 ggccaatcat cacggttgaa aggatagtgc gtctggctga agtaggtgct gccggcaatc
    18241 atccgggcca gctccttccc ttgggcatca accgcaaccc agtccccgtt gtccggtcga
    18301 aagtcgatgt gccggggaat ctggcagggc gaaccgtcgg gcagcaccca gtccttccat
    18361 ccgttcgggt cgaaatctcg gccggcgttg atcgcgtcga cgcgaaatcg ctcaagatag
    18421 aaatcctcgg gctcggcgag ttgctgaatg acgtcgaaca cgcgtgtctg gccttccttg
    18481 acgcccagat atgccttcag ccgcccgtag gccacagcct ggatgcccgt cgatcgcatg
    18541 gccccgcaat caatagggag ccggtccggc tccctgtgat cgatggccgc cagaatccgc
    18601 tcccgcgagg tcatcatttt gaggctcctc tcgtcagctt atactatacc ccgtcggccc
    18661 aaaggccgga agaatcctgg aaccttacgg ccagacgtgt gtgcagattg cgcgcttttt
    18721 tctgaatcgc agtgcgcttg ggttacggcc cgcaaaaagg cggtccgtct tggaatgtgt
    18781 gtgaaagccc acggagccgg ttggtagggc agtgtcaagg tcgccttggc gaccgagacc
    18841 tgccgtattc cgcagccggg acgaccgctt tcatacacct gcttgcacct gacggaagaa
    18901 cacatggcac aactgatctt caacggaaaa catgttgata tcgccgtggg tgagtcgctg
    18961 cttgcggcaa tcgcccgctc tggcgatgcg gctcatgcgg tggccagctc atgcccggca
    19021 gccggtcgct gcaaagaatg catcgtccag gtgctcgccg gaaacgaggc cctgaacgac
    19081 tggaccgagc cggaagcctt cctccgtcat caaagcgaca gcgaaaccat cattcatcgg
    19141 cttgcctgtc aggcaagagt cgctcagaca gatgccgtga tccgcgtcga ggccttcaaa
    19201 cgaaagctcg acatcgccgt gaacggtcgc ccggccgatg acgcatttga tccgtggatc
    19261 acctgcaacc agggacgctt gttttgcgac gacaagcagt tcggcgactg cgccggtcca
    19321 atctacggac tggcgctgga tatcgggaca acaaccgtcg tacttcacct tgtcgagttg
    19381 cagacgggca acaccctcga catgcgggca ttcgagaacc cccagcgctt cggcggcagc
    19441 gacgtcatcc atcggatctc gtacgacggc cataatccgg gccggcttca ccgcaccatc
    19501 atcgcttatc tcaaccgcga aatccgtgcg ctgccggtcg accccaaagg tatcgtagcc
    19561 gtcgtggtgg ccggtaaccc gacgatgcga gatctgttcc tcggcctcga cgtgcaatcc
    19621 atcggccggt cacctttcat gtccacaacc caggtcgatc tgctggccgg ccggcgccca
    19681 actaccgccg tgtggacgac cggtcaggag ttgggaatcg agatccaccc ccaagggcgc
    19741 gtctacgcca tgccgctgat ctccaaccac gtcggggccg acacggccgc agccctggcc
    19801 accctgccgc tcaacgacca accattcatg atgatcgaca tcggcacgaa cacggaggtc
    19861 gtcgccggct gcggtgatcg gctgatggcg gcctcatgcg ccgccgggcc ggctttcgag
    19921 ggcggacgcc tcggatgcgg aatggccgca tgtgagggag ctgtcacccg aatgcgccgc
    19981 cggccgggcg gctggaatct ctcagtcatc ggcgacggcc cggccagggg aatctgcggg
    20041 tccggactcg tcgacatcct ggccgagctt cgacgaacca acgagatcga cccactcggg
    20101 cgcttcgaca acggtcagac ccacgtgaca gtggccgaca accctcccct gcgatttacg
    20161 cgccgcgatg ccggcgagct ggcccaaacg aaggcagcca tcggcttggg gcagacggtc
    20221 ttgctgcggc acctgcgcat cagcccgcgc gacctgcggg catactacct ggcgggggcg
    20281 ttcgccaatc atatcgatct ggaaagcggc cggcggatcg gcttgttgct gcctgtgccc
    20341 gatgagcgcg tcattcgtat cggcaacgcc agcgtcgagg gcgccaaggg cgtgctgcga
    20401 agccgcttct gccgcgaccg aatcgagcat ctggtggggc gtattgaaca cgtcgaattg
    20461 gaaaaggagc cggacttctt cgacctgttc gccgagatga ctcaattgca gccgatcact
    20521 tgacgaacga tcgcccgctt ccgggccacg cgaccgaaac gccgccccga taccgaactc
    20581 gctcaccgca gtcgtttcaa tcgcggcctc ggccccaccg aggccccggg cacactcagc
    20641 ggcgccgcct tctgaacgtt gatccggtaa ttcacgctga acgacagtcc gccaagatcc
    20701 gcgccaatgt ccagaacctc agtcttggaa agcaccggat atccgccgaa cgcctcacct
    20761 tcgtcatcaa tgtaactgtc attattgaga tccgacccgg cgtaaagacg ataattgccg
    20821 gcgtagacgt catcaaaaga gaactcatag ttctccgaca cgtccgtctc atcctgggcg
    20881 taggcttcga gcgtgtcagg atccacaaga agcacataca ccgtgcccac gttgccgccc
    20941 gtcatcgtga tcgaacctac ggtcatccgg accgtgacgg tcgcattgcc gtcgttggat
    21001 acaaaggtga ccgagcccga gtagacgccg ttcgccagac ccgtgcggtc caccgtgatt
    21061 tcatagctgc cctccccgcc cgaggaaggg ctcaccgaaa tccagctctt ggacgaaacc
    21121 acactgctca cgatcagcgt gccgcccccc acgttgctca ccgtcacctc cgccgacgtc
    21181 aagaccgaac ccaaagagat gtcctgcggc acgacccgca gcaacggcgc gtcagacacc
    21241 cccgagcccg cgatttccga cgcggcccgg atcgcattcg gcgcgttgat caaaccatag
    21301 ccgaaatagg cgtcacgccc ggcaatccca agatcatcca cgatcttcac cgaagtcccc
    21361 gggtgcgttc cggccaggag ttggtcgaaa tcggcgggcg tcaggtccgg attcacggcc
    21421 ttcatcaggg acgccacgcc ggccacatgc gggcaagcca tcgaggtacc gtcataaaag
    21481 gcatacgtgt atcgcccgtc gtccttcctg atcgtgctga gcacgccgtc gctcacgccg
    21541 tcgccgtcgc ggtccgcatt cagatcgccc cccggtgcgc aaaccgccac cgtcgagccg
    21601 aaattggagt acgacgcgag atgacgaagc tggtccaccg cgctgacatt gactacgccg
    21661 ttgatcgccc cggggaacga attgtcggcg ctggtggcct cattgcccgc ggcggtgacg
    21721 atgatcgcac cggcctgccg ggctgcaaca acggcattgg tcagaacgct cgatgaaccc
    21781 ggcccgccga ggctcatgtt gatgatgtcc gcccgcctcg ccggcaacgt ccccgagtcg
    21841 ttgctcaaac gagccgcgta acgcacggcc tgggcaatgt cgtagtccgt gccctcgccg
    21901 gctcggccca gtacccgtaa aggcatgatg cgcgcgttcc aggcaactcc cgccacgccc
    21961 gtcgtgttgt tggtgcgggc ggcgatcgtg ccggcaacgt gcgtcccatg aaaactgctg
    22021 gaaataccgt agcccgccga atcgcccgga tcatccgggt ccgcatcgat cccgttgccg
    22081 tccatcgacg tggtcacgct gctgataaag tcgtagcccg tggtcaactg ccccgccagg
    22141 tcgggatgac cgagcgcaac gcccgtatca atcaccgcca cgatgacact ggagctgccc
    22201 gtggtaatgt tccaggcctc cggcagactg atcaggcggt agtgccactg gtaaccgtag
    22261 aattcgtcgt tcggcaccgc gaaggcgcgg cgtatgtagt tgggttcggc gcattcgacg
    22321 cccgcttgtc ggcgcagagc cttgattgcc gcaatggtcg gcgagatctc ggccgccatg
    22381 cctccccctg ccgcgcgagc tcgggccacg gacttgtatg cgtccatcgc ctcacgattc
    22441 aaccgcagcc tgtgaaaccc gccggagttg ctgccgtcgt taagctcttc cagaccgatg
    22501 cgcttctgtg tcgccgctcg cgcagacttg acggtctcag cgtacttgac cagtatctcg
    22561 ccttccgcgt gcggaaacag actcgatagc cgatcccgct gtgcctggac ggtcaccgat
    22621 gtggtcaggc tctcgccgac taacagtgag tagagaccgc cttggtcatt gtcgtgaata
    22681 acactgtaac cgcgaacctc gatcaggtag gtaccattgg caggagcggt cacttgctcg
    22741 gccttgccga gaccctccga actatcaagc aactgagcgc ccgattcgtc ataaagataa
    22801 aggtcaaaat cattatcatt cggatccgca agcaacaacg ttgctcgctg accgcccgcc
    22861 atctgaacca tgtacacgtc gcgcaggtcg tcactgccgg agatgctccc gagaaaaccc
    22921 ccgaccgtgc agggattggg cacggcctga gcaccgccgg gactgaccat gtcgttgtaa
    22981 acggtggggt tgctggcgtc ggctgtatcg ccgtccacaa caagcgtgtt gccgacactc
    23041 accgaaccgc gaacagagac tcttgcgacc ggggtagacg ggttcccgtc gcaatgacca
    23101 aactcgcttt ctccgtagtc tgagcatccg tcaccgtcgc tgtccacgac attgaaaggc
    23161 gtatccagag gatcaaaggg atccgttggg ggatcgttgt tgatctcttc cttatcagtg
    23221 aagccatccc agtcggagtc cacgtcaacc gtgacgcagc cctgtcccat agtcaacccg
    23281 atcgccggca ataacccgat agctgccgac aaccacagaa ggtcgtgact ccgcataccc
    23341 tttcctttca agatcgatcc aagacacgcc ctgtcaagca tttatccgga ccaagtgccc
    23401 ggcaagggct gcttcggccg tgtcatgcac gctgattcca actcctggac ggtaataggc
    23461 ataccctgat ctcgggccat caggaaaccg ccgcaaacat ttgtaatcgg ttgtttcgac
    23521 ctgcaaatgt ggatcttttt gtggagaccg gctttacagg aaaagtcgtc gacaaaccga
    23581 aggaaaccct tcaaaaaccg cgatgcttgc cgcaaaggct cgtttcaccg gaaggcgcaa
    23641 ccggtgggca aacgccgtca tccgccggca gggccgtcat gctaccctag agccacatcg
    23701 agagacatca tgatggcaaa cccgagcatg gtcccgaccg tggcgaagtc cgtgaacttg
    23761 tcacgctggg attccggaat cagttcctca accactacga agatcatcgc cccggccgca
    23821 aaggccaaac catagggcaa cagcgccttc atgctgaaaa ccagccaagc cccgaggacc
    23881 cccgccacgg gctcgaccat cccggacgcc tgccccatca gaaatgcctt gaaccggcca
    23941 acaccttcac ggcgcagcgg catcgacacg gccatcccct ccggaaagtt ctgcaagccg
    24001 atgccgatgg ccaacgccac gccccccaga gtcatgctgc gagccatctc aggatcaact
    24061 ccactgccaa tggcgccgaa agccacgccg acggccagtc cctcgggaaa attgtgcagg
    24121 gtgatcgcaa gcaccagcag aacgctgcgc tgccaggagg tcttgatacc ctccgcctcg
    24181 gatgtctcca accccaggtg caggtggggc agtatcttgt caatgatgta gagaaagaag
    24241 ccgccggcca gaaaccccac gaccgccggc ttccagtccc cctccgtcat ttcgattgcc
    24301 ggggccagaa gcgaccagta actggccgca atcatcacac cggcggccat acccagcaat
    24361 ccgtccatga ttttctggtt gaccttcttg aaaaagagaa ccggcgccgc acccgccgcg
    24421 gttaggaacc aggtgaacaa cgtggcgatc agggcctgcg tgatcgggtg gtaccggttg
    24481 aagaagctca gcaaggcctc attcatgcaa catcctcgac ctgcttgact ccaggaatcg
    24541 ctttttgtga aaccctgaaa cccctttccg agcacaaaac cggactgccg gctgacagat
    24601 acccgagacc gcacggccac cggagcctcg tgacctccgc tcgacaagcg ctctcgcttt
    24661 ccaccacggg ctcgccttat gcttccgaga actgatcctt gccgacaccg cactccgggc
    24721 aaacgaaatc atcggggaga tcttcgaacg gcgttcctgc cggaatgcct tgctccggaa
    24781 caccaacagc aggatcatac acccaaccgc acacgtcgca gacgtatctc ttcatcgatg
    24841 gttcctccca aaacaaaggt gccggagtaa gggcaccctc cactaccacg atcccggaga
    24901 tcaacgcgga gattctcgct tgcttcgcgt ccggccgcaa cccggagcgt cccgtcgtct
    24961 gatcgctgca ccgggggtcg ccatggccgg atccgcgatc atcccgcggc ggtacccacg
    25021 gtgcaagccc gcaacacaag cttcccgccg tgcaggccgc gagcgttacc atgtttcgga
    25081 gcgtctgcct gtgtcacgtc acaggcccgg cggagtgctg cgtcgagcta cggcgcattt
    25141 gtcaggcgcg cggaataccc tagtgatgag atcatctcga caagagattc cggattcaga
    25201 gtctgaccgg tcacgatcac ccggccgttg tgtacatcca cctcggtgga agagatcccc
    25261 ggttgatcgg ccagagtctt gcgaacgaca ttcgcacagt gctcgcatgt catcccgctg
    25321 acggccaact ccacacgctg atccgtgggc gtgaccgttg ccgcctcttc cgcccctgcg
    25381 gtgatcttct gccgccatga aacaatgtat gacacggcca gcacgcccag aagcaggatc
    25441 gcccacaagt gagaccggat cgcggccgcc tcgccgccat gcacgtgtcc gcccaaatcc
    25501 ggcaccctcg aacctgtgac ggtcacaatc gcatccaaca acaggccgaa accaatcgca
    25561 ctcatcgcca cggtgaccat gtacaacatc gctgatcgcc ggccgagcac cttccacaac
    25621 gtcgtgatcg tggccgcgtt ggtcgccgga ccggcgatca gaaaagcaag ggccgcgccg
    25681 ggggaggccc ccaagtgaat gaggccggcg gcgatcggaa tggaggcggt ggcgcacacg
    25741 taaaccggta cgcccgccgc catcagcaac agaatggaga ataggcctcc tccaatgtat
    25801 ggttccagcg agccccgcgg aatgaacgcc gtcatcgctc cggcgatcaa aacgcccgcc
    25861 atcagcgccg ctccgatgtc tcgcggcagg accgttagac catatcgcaa tccggaccat
    25921 aacgagccgc gcgccggcgg cttgaacgcg gcgccgcaac cgccctgctc cacgcaagac
    25981 gccgtcgcct ccgcacccga cgcgcgttct tcgcgtccct cgaaaaaccg gaccagcgtt
    26041 ccgccgagaa gacccgtcag gaaggccatg atcgggctga agatcgcgaa caccggtccg
    26101 agcacggcgt acatggccgc gatacaatcc gcgccggtct gcggagtggc cagaaggaaa
    26161 gccgtcgtcg ccgctcggcc ggctcccttg cgatgcatcg aaaccgagac aggtatcacg
    26221 ctgcaggaac atagcggcaa cggcaccccg aagagagcgc ccttgaccac cgctttgaaa
    26281 ccccgtcccc ccaaatgccg ctccacccac gcggccgaaa tccacacgga cagcaacccg
    26341 gccatgacaa agccgaacag caggtaagga gccatctgcc ccaacacctg ccaactcccg
    26401 agaacgaact gctcaacaag ctgcggcata ctgcaagaat tttagtgagg gccggcaccg
    26461 ctgaccacaa agccccgggc acgaacacgg gcgcgcattc aaccgtcata gacgccaccg
    26521 gcattggctt gcccagtcaa tgcgcgccgg tgaaggagac ttattgccgc ggagccaaag
    26581 acccaagggg accctgctgg ccctgaaaag gcgacaccag ctcagctccc gggatttctt
    26641 ccacccgttc gaagatcagt tggcacaacc tggtgactcg aggtgtcact cgcagcgata
    26701 tggggccgtg gttcatgatc tcaagagtga tcttgccttg gaaactgttg tggatcgtcg
    26761 gagcagtcat atgaatcacg agcccgagtc gagcgaatcg acttcgtcct tccacccggg
    26821 ccgccggctt gccgtgcgat ggcaacgcga ccctctccag ggtcgtggcg atgacaaact
    26881 gcttcggcct cagaatcacc gtgccattcg actccgcagg caggggttcc atgtaggggg
    26941 ccagatccgg gatctgaacc tcgtcgagat cgaagctgac ctgcacgcct ttcgtctcgt
    27001 acaacttggg ctggaattgg cgaaagtcat cgccgatgtg aagatccagg gcggtaggag
    27061 agaactgatc gtctgccgga ttcggctcga tgagcaaatg cccgtcttga atggctttcc
    27121 gaatctatct atcggtcaga atcagcgccc gacgtctccg tcaagcggac agaacacact
    27181 cagccggcac gctgaccttc atggcatcgc tgcctccgga cgcaggaatc agtacaacat
    27241 aggagtcatc ggactctccc accttccata ctcgaacact cgcatcgcag ggcggtgctg
    27301 ccgcgccggc cgtcaccatc acgccatccg cgtcagagac gaactcgatc tgccgacggc
    27361 tgcccgaaga aaccactgga aaggtaacaa tgtattcact cttgaacaga ccgggtcgaa
    27421 ccttgcatct gaccttcaac tcctcgacca cgacgcgcct cctgctatgg caggcaacac
    27481 catcatctcc ctcatacgcg cagctttcac cataaccgtt ccataggtag atacaagcgt
    27541 gggaaacgac ttcaaaacgg ctcctgcttg cccggcatga gtacgcttgg cgtcgtcggg
    27601 gtggcccaac tacaatcgca ggggtgagcg gcgagcgaat cgacaccgtc tgggatcgcg
    27661 atcgactgca gtcgccccat gcgcagccgg acaaggctca gcgcgtgaga cggatgtttg
    27721 atgccatcgc gccgacgtac gagcgcatca acacgatcgc cagcgcgggg cgcgaccgct
    27781 actggcgacg cgagatggtt cgactggccg
//
LOCUS       DAOTPW010000038        27676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1798_length_27676_cov_5.968176, whole genome shotgun sequence.
ACCESSION   DAOTPW010000038 DAOTPW010000000
VERSION     DAOTPW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27676
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1798_length_27676_cov_5.968176"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="P5316_05260"
     CDS             complement(<1..519)
                     /locus_tag="P5316_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRR84399.1"
                     /translation="MQRDRQSMGNRMSDNPMTSFISSLCQHAGITIIATDEQFVIRFW
                     NSAAERMFGQPASAAIGQPIWLIIPPERHGLAQRMLERAVTRGETSEVELRRTGPDGR
                     KHYLAVTISPVPDEHHHVVGVSICVRDVTKGMDLMRDVAETQRMSALGSMSGAIAHHF
                     NNLLGGALTALDF"
     gene            complement(529..1413)
                     /locus_tag="P5316_05265"
     CDS             complement(529..1413)
                     /locus_tag="P5316_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84400.1"
                     /translation="MRTRPLLIGAILIASGCSDWPTWPNQAPPSPTTTPSADLGQPRT
                     PSIAGIGEQLTDNGPRRPTMILQISFEVLRARVPRGFFSQSEKIWNHVNEAVVPQDVA
                     VLLQRNGLRAAVGGTDSWPPIRALLEQQGPVETSQNRMILHNGLPLVVEPDQRTRNQT
                     LFMFRPDGTLGGASFPQSVNGLLIEYQLAGAAAEDLRISVTPQVRLPMQAPRLTHNVP
                     GYIDQPVVPPTRVFRELAFTVTVAPGQFVAIGPSRLIENAHLVGSLLLCDEREGEKME
                     SMYFITPQVLNTGRRVGF"
     gene            complement(1588..2676)
                     /locus_tag="P5316_05270"
     CDS             complement(1588..2676)
                     /locus_tag="P5316_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRR84401.1"
                     /translation="MQPSTLRAPNVEGNLPGFARRAARLFATPCGMLFVFLGGMLPGV
                     TAITRKLDIPALACLAGATLCWGSVPVMLGYLADPNLVPDGYTANAVRYPISALAYLP
                     WLVVAIRRGGLGRFWLTALIPSAVNVAGQTLWGLAPYYLPPGQFAFLFRLSALFGVLG
                     AFWLFRDERRLARSPLFWVGAILATIGFGTMSALILTGGTAIRPAGIVIIFLCSLCYG
                     AYGVAVRYVMGKLDPLVVFAVICAYTSIPLLAMAPLGNPASVLGLKAVPMIVLIVSGL
                     LGVAVAHGLYYIAVQRLGVVVTSMTLTATPFVSIVGSSIWLGERFTAGLWVAGSVLAI
                     GTGCALWSQQHLPEAPKDEAMAETAGQP"
     gene            3056..4027
                     /locus_tag="P5316_05275"
     CDS             3056..4027
                     /locus_tag="P5316_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR84402.1"
                     /translation="MSSSAGDFVIEDFTPAYLAPEVYRELSGRVAVAIESLRCCQMCP
                     RRCEADRLSDGIGFCRTGRHAAVCSAFAHTGEERCLVGRHGSGTIFFGRCNLRCVFCQ
                     NWDISQEESGTPCKADDLADIMLALQEKGCHNINLVTPEHVVPQVVEALAIAAEQGLE
                     LPVVYNTSGYDSMESLRLLDGLVDIYMPDFKLWSPDLCAEYLNARDYAEHTRQAIVEM
                     HRQVGDLTFTPDGVACRGLLVRHLVMPGLPDESGRILEWLAGEVSPNTFVNIMGQYRP
                     EHLVGRMCEDPNAAGGVRYPKINRRPTRGEIEWAYEAARKAGLWRFD"
     gene            complement(4059..5741)
                     /locus_tag="P5316_05280"
     CDS             complement(4059..5741)
                     /locus_tag="P5316_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HRR84403.1"
                     /translation="MLSKSVSLAAAVLVVFSQSTIGQQPLRLEIWAVDPLVKVFRDAT
                     PAAGTEALAEVACGEHATFQIVVRSEQPVTALRASVAPLALETPMGVYRQADKPRFVG
                     YVPVDRPMQTPSKDQLRKPPADYPDPLLEVDSIDLPAGQAQPIWITVPVPVHSAVGTY
                     RGSLNVTGQIENAFVRAQIPLVLKVHRAIIWKSRLWTTNWFGMHWRHMQISPKDDSPE
                     YWELLARYARNMAEHRQNVALISPLALAEFKADEGNKLQIDFSKFDRWVKIFMDEGVI
                     GMIEGGHLGGRAGGWESQFVVQIRQVKDGKVVPSSVDPGSPEADAFYARFLPALVNHL
                     KEKGWSNIYVQHLADEPIKENIATYRAIAALVRKYAPGIKIIEACHTKDLVGAIDIWV
                     PQLNFLHQDYQHYQDRRKAGDEVWFYTCVFPQGEYANRFIELPLIKTRLLHWINYRYG
                     ITGYLHWGYNWWTEEDPFKKVTREHGGPPFLPAGDAWIVYPGKNGPLDSIRFEAMRDG
                     IADYELLCKLGEKDPEAAKTLVARHVLDFDKYETDVAAFRAARRELLEKLSK"
     gene            6100..6468
                     /locus_tag="P5316_05285"
     CDS             6100..6468
                     /locus_tag="P5316_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR84404.1"
                     /translation="MEELPNVTTIPAANGMEALALVAREQPDLILLDIMMPKMSGFEV
                     CRHLKSDPQTRDIQIMMVTALNELGDHERAAECGTDEFLSKPVNRVELTTRVKSLLRI
                     RHLKQELQRTLESMGNKSQA"
     gene            6520..7125
                     /locus_tag="P5316_05290"
     CDS             6520..7125
                     /locus_tag="P5316_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRR84405.1"
                     /translation="MSADKDYLSLPLRTFVADLAAKRPTPGGGSVAAMAGLLAAAQAR
                     MVVEYTVGKPKFAAYEKKLREALGELERAEAAFAELMSEDMAAYERLAASCKAGDPAE
                     QQQAVATATAVPMEITAMAGAVLALLDGLKECVNPYLLGDLKVAAIMSLAAAQSAAVN
                     VEVNLRDLADGSEVERVNKEMTRILEKAYDHRNSVLNQRAS"
     gene            7139..7885
                     /locus_tag="P5316_05295"
     CDS             7139..7885
                     /locus_tag="P5316_05295"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR84406.1"
                     /translation="MMRSLWVFAVLVGVAWAVALWFNRRGVCRFDGSGDPNRGIVVFV
                     EPVRWLFIVWCFNALCRALRRAGRTDAVYLYRWSRAAGSVLVLPDLMRRHRLDAKAVR
                     LAQFIEQLRREHPASPIHLVGYSTGVYIAFESLRQMSPATTIGQVVALHGTVSPDYDM
                     TEVTRRAKGVLTVHGRPDWLINGLGPLLFGTNDRVHTAACGMVGLRSAPAKVEQRGWQ
                     ISDARAGYLGDHFTVMSRGWLRVNIISRLT"
     gene            8049..8297
                     /locus_tag="P5316_05300"
     CDS             8049..8297
                     /locus_tag="P5316_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84407.1"
                     /translation="MATEPPYYLDIKGIEGEPPEPEGPQREPRRWIGVEFECCGVYTR
                     IYKNREGTAYEGKCPKCLRPVRVRVGPGGTSHRMFRAR"
     gene            complement(8779..9678)
                     /gene="scpB"
                     /locus_tag="P5316_05305"
     CDS             complement(8779..9678)
                     /gene="scpB"
                     /locus_tag="P5316_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRR84408.1"
                     /translation="MSADREPTPDQQPVEDGLPADVAASEAIADALPADSDPVWPESG
                     LTEIAPDLQAEPAEQGGRLMTIAGGRLVGDSEEPATDPTAGRLAEALAALSQETAETA
                     DADESKEAGPSDDADAAPRAGEQELTTERVIEAILFATDTPLTLARIVSILGVGSARD
                     VRKHINALNKRYDQQGSAFRIEMIAGGYQMLTRAEYNTWLRRLRQNKQDSRLSQAAME
                     TLAVIAYKQPVVRAEIEAIRGVQSGEMLNRLRELGLVKIVGRAEDVGRPMLYGTTKRF
                     LEVFGLSSLEDLPEVEELKTDEE"
     gene            9861..11129
                     /locus_tag="P5316_05310"
     CDS             9861..11129
                     /locus_tag="P5316_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR84409.1"
                     /translation="MSMCRTPAWSSGIRQEKTMVWAAKGKSPFYPGQPVPVEFFVGRT
                     EQIAHIMQRGAGQVAAGKPVAMFVQGEYGIGKSSIAGFVQRLAEREYGLHAVYAPLGS
                     ANFLDDVGASILESMLRSGAFDRSRSERIRNWLAKYVGQQELFGLTIHTEALRRDAPT
                     VAKPSGVLAFLGEALRRLGETGVRGLFLVLDEINGISAAPKFAHFIKENVDANAMARE
                     PLPLLLMLCGVEERRRDMIKSHQPIDRIFDVVEVERMTVPEMEGFFRKAFDSVQIAVE
                     PEAMKLLTEYSAGFPKIMHIVGDAAFWLDQDGVITEQEAWRAVPNAADEVGRKYVDQQ
                     VYSVLRSRDYRSILGTIARTGLDMGFRRADVARGLTDTEKRKFDNFLQKMKRLNVIRS
                     GDLPGEYVFNVRMVRLYIWLDSLRKNHAAR"
     gene            complement(11269..12699)
                     /gene="purF"
                     /locus_tag="P5316_05315"
     CDS             complement(11269..12699)
                     /gene="purF"
                     /locus_tag="P5316_05315"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRR84410.1"
                     /translation="MSSPSLKHNCGLFGVYGHPDAVLLTYLGIYAQQHRGQEAAGICS
                     VGGGGPIQRYANLGLVTTVFHNKRILDELRNPHAIGHVRYSTTGSCHNINCQPILVNY
                     ARGQVAVAHNGNLINAGLLRKEFEEQGAIFNTTSDSEVILHLLARPAHLDKANVIPHI
                     LGHLQGAYSLLFLFPDRMVAARDPFGFRPLVLGTLENGAIAVASETCAFDIINARFLR
                     EVEPGEVVTISEKGMESSRIVPEDAFRPAYCIFEQIYFADPASDVFGENVHKVRQAMG
                     AELAREAPVEADAVIPVPNCARCAAMGYARASGIPYERGYTTNHYVGRSFIMPEQVQR
                     DLVVRMKLNVIKENVRGRRLIVVEDSVVRGTTTRGKIGTLRQAGAKEIHLRVASPPIR
                     HPCHFGIDFPTQQELIAHQRSVAEIRDYLGVDSLHYLSLEGMLKCVKHPGRYYCTACF
                     SGRYPMPVDQQIGKFALERYQLKMFE"
     gene            12871..13797
                     /gene="rbsK"
                     /locus_tag="P5316_05320"
     CDS             12871..13797
                     /gene="rbsK"
                     /locus_tag="P5316_05320"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRR84411.1"
                     /translation="MMASSPVVIIGSITMDLVVRTPYIPVGGQTVSGRGFTAIAGGKG
                     ANQAVAVARLGEPAVMIGRVGDDAFGATLLCDLKSNGVDCTWVQSTPGVASGITVISV
                     SDSGEKAVTVVGGANLLVTPTDVDAAQEVIKKARICLLQLEIPLETVKHAIEVCRRHG
                     VETVLETAPVPRDGLPDSLLQADVVSLNDQEAAQLTGLSAIKSPAAIAAALSKRGCRS
                     VVLKLGQHGAYVFSPEGESAVPGFAVRVVDTTAAGEAFTAALAVARSWGWKLIEAIRF
                     ANAAGALACTRTGAQRSMPALSEVEALLAGQP"
     gene            14023..14601
                     /locus_tag="P5316_05325"
     CDS             14023..14601
                     /locus_tag="P5316_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR84412.1"
                     /translation="MGKLKDVLTTGDVARICNVAPRTVSKWFDTGQLRGYRIPGSKDR
                     RIPLAQLIRFMKAHGIPLDHLETGRPRILIVDADVDLARLLEKALEERFDYEVMVASS
                     AFEAGALMEQCKPAVVLVDIDVPGIEGRVLFRFIAARGELQGTQVVAMGAAITDADRQ
                     SLLQQGYHNTIAKPFNVQQLAALVEESLAVGI"
     gene            14667..15383
                     /locus_tag="P5316_05330"
     CDS             14667..15383
                     /locus_tag="P5316_05330"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRR84413.1"
                     /translation="MSTSSAPDPLSSALFGKTRRAVLALLLSHPDRSFYLREIARQVG
                     TGLGAAQRELTRLAAAGVIRRISQGHQTHFRANPECPVFAELRGIVLKTSGAAEVLRE
                     MLWPLRDRIRLAFIYGSIARGEPREGSDVDLCVIGDTTFSAVVAALGGAHDLLGREVN
                     PTVYPPDEFRRRLARKDHFLYALSRGPRIVILGDERELAGMAEQRLAGPSSDEPARDS
                     RPARSGRPRPRRRTNAAAQR"
     gene            15334..15702
                     /locus_tag="P5316_05335"
     CDS             15334..15702
                     /locus_tag="P5316_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84414.1"
                     /translation="MVDRDLADAQTPRLSADWRLNIAYNAALQAATAALAAAGCRASR
                     EAHHFRVLQSLRFTIRLEENAIRELEQFRKKRNIGGYERAGAVSDQEAAEMLLLAQKL
                     RRAVVNWLKSAYPQLVPSGD"
     gene            complement(15747..18083)
                     /locus_tag="P5316_05340"
     CDS             complement(15747..18083)
                     /locus_tag="P5316_05340"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HRR84415.1"
                     /translation="MGKHRRSSAAGLLGVFFLVVVFPARGATERVLARWDFDSRSGWN
                     GWLPGGTIKNVEFGPDGVAFDALGPDPIIVSPEFELSPATNRQYVTIDLDCSAAGRAE
                     LFFTNKTTGPYGGFEPKWVNLAFVPSGGRQSLTIWPFWGVLQKVIRIRFDPPSGVRCR
                     LSSIRIVDASQSGGPPAWSFRDANDPWRPMCASTAERTGEGLVVRATAPQASIAVVVA
                     PFAAERRFILRLDARCPGEDVLGFYWATDDQPGLIGRPIPIAGEGHARSIELDLRCFP
                     EWKGTVTHLALAFGTHGSETLTLRSMSIDENDATRPFLRLRHMGFERPVSRPGKAAGV
                     QAVLEHVGGPAVPAGVASFAVDENVRCEKPSVSMPAVEPGKRAEVATEIIPSKPGSTS
                     VTLHANGQTFTRILHVDEPITDPTLLDRGSDNYDVPKPRPVKTRYQIGVYYFPGWSPD
                     QISRWQKQADFPERDPVLGWYKEGLPEVADWHVKWAVENGISFFVFDWYWRDGKEELG
                     RALNDGFLNARYRDYMKFAVMWANHPPFAGHTPDQLLAVTEYWLKHYFRRPNYLTRDG
                     KPYVSFFSVHELLTDLGGPEKTKAAFDAMRRRVRDAGLAGLHIAACTGNDSHSIEMVR
                     KAGFDSMTAYNYSGAGATMPQSSYRSFLLGHESIWKAMRQAGNPPYVPLLTVGWDPRP
                     WHGPRTNSRFARRTRDFAEALGRLKVDLDMNRERMALLEAWNEWGEGSYIEPNAEFGF
                     RDLEAVRRTFAEPGDWPVNVAPQDLGLDGKYDLRAEKK"
     gene            complement(18261..19652)
                     /locus_tag="P5316_05345"
     CDS             complement(18261..19652)
                     /locus_tag="P5316_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRR84416.1"
                     /translation="MTTTAFDQSDLAVGSEAMRFFPVEVATIEDGTLEMDVYLDSAGD
                     GSSYVLYCAKGAAFTSKLREKLIPRNVRFLYVPMSQHKEYRKALARYLEKTYTDPHRE
                     RSERIRIVREACTKMIDDVLLFPERPESLETVKDVSRQFAEWVDEDQVGFSYLMDMAT
                     HDYYTVTHMVNVGVGCGLLAKQLKPDDSQWHAALIEGGLLHHVGKRRIPTEILNKEGK
                     LSPEEWELVKSHPGTGFDLIKTNPAMSQIVMDMVHSHHERFDGAGYPIGLAGEQISEE
                     ARICSVIDVFDAICAARPYRGPLPPEQTLKVLAEGKSTCFDPEMVDAWDAIVRGLLEA
                     DPQRVPPQGAANESLSLDSFVQQSPSGGTRAEPINRRRHPRFECCLNARARWLDKRTE
                     HPGKGDMWAEVIVLDISQGGARISMALPIEVDEVLELEVPAKGRPPVRRVGRIVHVAT
                     ESGPRWRAGMVFL"
     gene            complement(20292..21098)
                     /locus_tag="P5316_05350"
     CDS             complement(20292..21098)
                     /locus_tag="P5316_05350"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRR84417.1"
                     /translation="MGLPPDQIRGSLERYLEIDRLLGIDSAPLSPRPPRHAQLSGKHP
                     PAAASPGRDKPVVALQALSPEELLRRQEALKTLDAGQVKGCMKCRLSRTRTQTVFGQG
                     NPAARLVFVGEGPGADEDAQGLAFVGRAGQLLTRMIEAMGLTRDQVFICNIVKCRPPE
                     NRTPAPDEISACWPYLEEQLRIITPEVIVALGKPATQTLLGTTEGIGRLRGHWHEFFL
                     SGSPMIGPATPLMPTFHPAYLLRDPGKKPQAWSDLQMVMARLGLPLPERR"
     gene            21534..21839
                     /locus_tag="P5316_05355"
     CDS             21534..21839
                     /locus_tag="P5316_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84418.1"
                     /translation="MAFDSNRYLAVGSKVRVAEPMEVPDWSTWDDDHGRISSQIKKRL
                     QTLFFKGNDKITAEVVYIAKESEREKLRRLGRIKLRLRDQSGCALVITADPKHLESA"
     gene            21836..22474
                     /locus_tag="P5316_05360"
     CDS             21836..22474
                     /locus_tag="P5316_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84419.1"
                     /translation="MTCSGPPGGYHHLVMSDAGVVLIPHSLTEARLYVKTRRCASCGE
                     GPLLISREAPVYDAAAHGLTVTVVCDECGQPASIRFCVAELERRGPVLSMLDELRDVS
                     QQPAAQVINPTRRPSRLIDVAGWVMLYTMLTEAARTESVKSTSIERRAAVRRMRIEAA
                     LCLDEALKFFDEDSDLPPREAFFTKESYGRFLDRPELYTRQRLIEMRGQSPR"
     gene            22471..22605
                     /locus_tag="P5316_05365"
     CDS             22471..22605
                     /locus_tag="P5316_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84420.1"
                     /translation="MSHPASKHGKLPGWYLLDCGTSKPVPLIRFRLGLPKATGPMLLR
                     "
     gene            complement(22597..24225)
                     /locus_tag="P5316_05370"
     CDS             complement(22597..24225)
                     /locus_tag="P5316_05370"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HRR84421.1"
                     /translation="MKARISRRIYLKSLACGAAAALGAGSRARLFAAESKDSRATLDI
                     LLDEQIREPDGKPAVISPLLHGHFTEHIGGVIYDGIWVGPDSRVPNVDGIRKALVESF
                     KKIAPPVIRWPGGCFADRYHWRDGIGPAAGRPRRFNRWGERGEPNAFGTHEFIKFCRL
                     VGAEPYLAANVGTGTAEEFQQWVEYCNAPAGSTTLADERNANGDHEPFKVRFWGVGNE
                     NWGCGGTYTPEDYCTEYRKFTTWVPKYGVPLYLVACGPNGGDVSWTRRFLKKWRDAAT
                     APISGFAGHYYCGSSGTATQFNAAQWYDLLDKANYMETFINEHWKAMGEFDPKHEIKF
                     IVDEWGCWHPSGTEIKKEFDFCQISTLRDALVAGLTLDIFHRHAEKLSLCAVAQLVNC
                     LQSLYLATDDKFIETTNYHVFSMYLDHQNGTAVPLRVAADPVTFSLDGKDRKIFRVAG
                     SASVRDRQLTLTLTHTHASEPIEVAIKLVGSARPAELAGTVLTHSKLNAANTFESPAE
                     VIPRDLPLDRPKPDGSLVVTLPPASVTRLRIPLS"
     gene            complement(24333..24794)
                     /locus_tag="P5316_05375"
     CDS             complement(24333..24794)
                     /locus_tag="P5316_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84422.1"
                     /translation="MSFQNDVEQTAGLEHAYRPGKQALRPADQNKVDAIATRRLAGSA
                     DIDAALENLRPNENRWDYVVGWATKEKSEFLYWIEVHSASGAGAVREILDKLTWLKGW
                     LDGEGNRLRNYRRKIVWIASGKSAFQQNSPQLKRLVAAGVYFAGGHYTIAD"
     gene            complement(24791..25957)
                     /locus_tag="P5316_05380"
     CDS             complement(24791..25957)
                     /locus_tag="P5316_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR84423.1"
                     /translation="MPPSLELRNIGQIQEANLSFGDLTVLVGPQASGKSIALQWLKLV
                     LDTGLVQAQLSQYGLDWGKSVSKFLDLYFGEGMHTVWQEGLSEVLWDHQPWDANKKIS
                     RKAVSKKESVFLVPAQRVLTLRDGWPRPFSDFSAGDPFAVRSFSEKLRLLMEQELGAG
                     DAVFPKSNRLKSEYRKLLEKTIFQQFRLKVDALRSQKRLVLGQTAGTAPLPYMVWSAG
                     QREFVPLLLGLYWLMPPAKVSRRNDVQWVIIEEPEMGMHPRAITAVLLLVLELLDRGY
                     RVCISTHVPQVLDLVWVIQKLGRTQAKPEKLLQVFEAPNTQGLRTVAANALRKECKVY
                     FFDGDSGQVHDITGLDPASLDKTESSWGGMLDFSGRANEAVATAMANTGTLFPL"
     gene            26390..27631
                     /locus_tag="P5316_05385"
     CDS             26390..27631
                     /locus_tag="P5316_05385"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRR84424.1"
                     /translation="MKEKLFVGSWLLAQVLVTGMALGAESWTAPTVRLPAAERHPVIA
                     CAPEELARLRAAYQAGGPAHVVAANIVKATDKFLDDPVTFPPRGGQHNQWYQCDKCEL
                     GLKTLSDTQHQCPKCGKIYTGEPYDDVVFKRHHSRNLSQMRQAAWAYAITGDRKYADY
                     VVKILLGYADRYLKYPYHTNSATKQPKGLSGGHIDEQTLGEATALSRQIAPAYDLIHD
                     SPALSDADRKAVEEQLILPMLKNIDKHKAGKSNWQTWHNAAMLWGGAVIGDEAWIRKA
                     IEQPGQGFAFQMSHCLSEEGMWYENSWGYHFYTLDALVETCEGARRLGIDLWSHPNLK
                     KMFLLPIRYAMANGSLPRFGDDVNASTQRIGSLGEPAYLVYREPLLVPYLSKTPTPEN
                     IMLGRKIERQALEHKPSPISP"
BASE COUNT         5384 a         8192 c         8876 g         5224 t
ORIGIN      
        1 gaagtcgagg gcggtcaggg cgccgccgag aaggttgttg aaatggtgag cgatcgctcc
       61 ggacatcgac ccgagggccg acatcctctg ggtctcggcc acgtcgcgca tgaggtccat
      121 gcccttggta acatcgcgga cacagatcga gacgccgacc acgtgatggt gctcgtcagg
      181 cacaggggag atggtcacgg ccaagtagtg cttgcggccg tcggggcccg tccgccgcag
      241 ttccacttcg ctggtttctc cgcgtgtcac cgcccgttcg agcatgcgtt gggccaagcc
      301 gtgacgttca ggcggaatga tcagccagat cggttgtccg atcgccgccg atgccggttg
      361 tccgaacatc cgctccgcgg cgctgttcca gaaccggatc acgaactgct catccgtggc
      421 gatgatggta atgcccgcgt gttggcacag gctggagata aaagatgtca tcgggttgtc
      481 ggacatccgg ttgcccatgc tctgacgatc gcgctgcaag atagcgcctc aaaaccccac
      541 tcgccggccg gtgttcaaca cctggggcgt gatgaagtac atggattcca ttttctcccc
      601 ctctctttca tcacacaaca ggagggagcc gaccaggtgg gcattctcaa tcagccggct
      661 ggggccgatg gcgacgaatt gccctggggc tacggtgacc gtgaaggcca attcccgaaa
      721 cacacgcgtg ggagggacca cgggctggtc gatgtagccc gggacattgt gagtgaggcg
      781 gggagcctgc atcggcaaac ggacctgagg cgtgacggaa atgcgcagat cctccgccgc
      841 cgcgccggcg agctggtatt cgatcagcaa tccgttcaca ctttgcggaa agctggctcc
      901 acccagcgtt ccgtcggggc gaaacatgaa caaggtctga ttgcgcgtcc gctgatccgg
      961 ctcgaccacc aggggcagtc cgttgtggag aatcattctg ttctgggagg tttccacggg
     1021 cccctgttgt tcgagcaggg cgcggatcgg cggccacgag tcggtgccac ccacggctgc
     1081 tcgcaatccg tttcgctgga gcaacacggc aacgtcttga gggacaacgg cctcgttgac
     1141 gtgattccag attttctcgg attggctgaa gaaccctcgc ggtacacgag cccgcaacac
     1201 ctcgaaactg atctgaagga tcatcgtcgg gcggcgcggc ccgttgtcag tgagctgctc
     1261 gccgataccg gcgatggaag gggtgcgggg ttgtccgaga tcggccgagg gtgtcgtggt
     1321 tggtgagggc ggggcctggt ttggccaagt cggccagtcg ctgcaacctg aggcaatgag
     1381 gatcgcgccg ataagcagcg gcctggtccg catagatttc gaaccccgag cctgaatcat
     1441 gagtgggtga cgtggaatag tgacagcgtt tctcccagct tgcaggtcat gacgacgggg
     1501 gggatgacaa gttgacgaaa tcggcagcat ggcatggtct aaccatacgt gaaatatggg
     1561 cttaggtcaa tcgaccagcc ggattggtca cggttggccg gcggtctcgg ccattgcttc
     1621 gtctttgggg gcctccggca ggtgctgctg tgaccacagc gcgcaaccgg tgccgatcgc
     1681 cagaaccgat ccggccaccc acaatcccgc tgtgaaccgc tctcccagcc aaatcgacga
     1741 gccgacgatt gacacgaacg gcgtggccgt cagggtcatc gaggtgacca ccactccgag
     1801 ccgttgcacg gcgatgtagt agaggccgtg cgccacggcc acgcccagca gcccggagac
     1861 gatgagaacg atcatcggca ccgctttcag gccgagaacc gaggccggat tgcccagcgg
     1921 ggccatcgcc agcagcggga tggaggtgta cgcgcagatc accgcgaaca cgaccaacgg
     1981 atcgagcttc cccatcacat agcggaccgc aacgccatag gcgccgtagc agagcgagca
     2041 caagaatatg atcacgattc cggccgggcg gattgccgtg ccgccggtca gaatcagcgc
     2101 ggacatggtg ccgaatccga tcgtcgccag gatggccccg acccagaaga gagggctgcg
     2161 agccagccgt cgctcatccc gaaacagcca gaaggcccca agaacgccga acagggccga
     2221 aaggcgaaag agaaaggcga actggcccgg aggcaggtaa tacggtgcca gcccccagag
     2281 cgtctgaccg gcaacgttga ccgcgctggg aatcagagcg gtcagccaga agcgtccgag
     2341 tccgcccctg cgaatggcca ccaccagcca cggcaggtac gccagcgcgg agatcggata
     2401 ccgaacggcg ttggcggtgt agccgtccgg aacgaggttc ggatcagcca aatagccaag
     2461 catgaccggg acgctgcccc agcagagcgt tgcaccggcc aggcaagcca atgcagggat
     2521 gtcgagtttg cgagttatgg ccgtgactcc tgggagcatg ccgccgagaa acacgaagag
     2581 catgccacat ggcgtggcaa acagccgtgc cgcacgacgg gcaaagccgg ggaggttacc
     2641 ctcaacgttg ggtgcccgca acgttgaggg ttgcaaattt cagatcaaag acggcggggg
     2701 tgcatccgcc ggactgtggc agcccatcgg cagggcaaac gccgcggtgt gccgccgccg
     2761 cgtcgtccaa aaccccagca ctgctcacaa agcggccgct ggaccgagat tcccatctcg
     2821 gtcccctctg gtgcggattc ccatccgcat gggtgccgaa tgcggaatgg attccgcgaa
     2881 ccaagcgacc gagaagggat tctcggtcga gcggtcgaga gcggactctc ggtcgagcgg
     2941 aattcaataa gggcggacga gtgcgtcggc cccactgtgt gttgaacgga gattcgccgg
     3001 gggggggctt cggcggtaca atgggcatcc gctgcaaaca cgaagggtcg gtttcatgtc
     3061 gtcgtcagcg ggagatttcg tgatcgagga cttcacgccg gcctacctgg ctccggaggt
     3121 gtaccgggag ctgtcggggc gtgtagcggt cgctatcgag tcgctgcgct gctgccaaat
     3181 gtgcccgcgc cggtgcgagg cggatcggct gagcgacggg atcgggttct gccgcaccgg
     3241 ccgtcacgcg gccgtctgtt ccgccttcgc ccatacgggc gaggagcggt gcctggtcgg
     3301 ccgtcacggc agcgggacga tcttcttcgg ccggtgcaac ctgcggtgcg ttttctgcca
     3361 gaactgggac atcagccagg aagaatcggg cacgccctgc aaggccgacg atctggccga
     3421 tatcatgctc gccttgcagg agaaaggctg ccacaacatt aatctggtca ccccggaaca
     3481 cgttgtgccg caggtggtcg aagccttggc catcgccgcg gaacagggtc tggaactgcc
     3541 ggtggtctac aacaccagtg ggtacgactc gatggaatcg ctccggctgc tggacggtct
     3601 ggtggacatc tacatgccgg atttcaagct ctggtcaccg gatctgtgcg ctgagtacct
     3661 caatgcgagg gattatgccg agcacacccg gcaggccatc gtcgagatgc accgccaagt
     3721 cggtgacttg actttcacgc ccgatggcgt tgcctgccgc ggcctgctgg tccgccacct
     3781 ggtgatgccg gggctgccgg atgaaagcgg tcgaatcctg gaatggctgg ctggtgaggt
     3841 ttcgccgaac accttcgtga acatcatggg ccagtaccgc ccggagcacc tggtggggcg
     3901 catgtgcgag gatccgaacg ccgcaggagg tgtgcgctat ccgaagatca accgccggcc
     3961 tactcgcggg gagatcgaat gggcctatga agccgcgcgc aaggcgggcc tgtggcgatt
     4021 cgattgaact ccggcgtcgc agacggcagc gccggggctt acttgctcag cttctccagc
     4081 agttcgcgcc gggcggcccg gaaggcggca acgtccgttt cgtacttatc gaagtcgagg
     4141 acatgcctgg cgacgagggt tttggcggcc tcggggtctt tttcgccgag tttgcagagc
     4201 agttcgtaat cggcgatgcc atcgcgcata gcctcgaagc ggatcgagtc gagggggccg
     4261 ttcttgccgg ggtagacgat ccacgcgtcg ccggccggca agaagggcgg gccgccgtgc
     4321 tcgcgcgtca cttttttgaa cggatcctcc tcggtccacc agttgtagcc ccagtgcagg
     4381 tatccggtaa tgccgtagcg atagttgatc cagtgcagca ggcgggtctt gatcagcggc
     4441 agctcgatga accgattggc atactcgccc tgagggaaaa cgcaggtata gaaccagacc
     4501 tcgtcaccgg ctttccggcg atcctggtag tgctggtagt cctggtggag gaaattgagc
     4561 tgcgggaccc aaatatcgat cgccccgaca aggtccttgg tgtgacaggc ctcgatgatt
     4621 ttgatccctg gggcgtactt gcgaaccaaa gccgcaatgg cccgataggt ggcgatgttt
     4681 tccttgatcg gttcgtcggc caggtgttga acatagatgt tcgaccagcc tttttccttc
     4741 aggtggttca ccagggcggg caagaaccgg gcgtagaacg cgtccgcctc ggggctgccg
     4801 gggtcgaccg aactgggcac gactttgccg tccttgacct gccggatctg aaccacgaac
     4861 tgcgactccc agccgccggc acggccgccg aggtgtccgc cctcgatcat cccgatcaca
     4921 ccctcgtcca tgaagatctt cacccagcga tcgaatttcg agaaatcgat ctggagcttg
     4981 ttaccttcgt cggccttgaa ctccgccagg gccagcggcg aaatcaaggc cacgttctgg
     5041 cgatgctcgg ccatgttccg ggcataacgc gccagaagct cccagtactc aggcgagtca
     5101 tccttcgggc tgatctgcat gtgtctccaa tgcatgccga accagttggt ggtccacagg
     5161 cgcgacttcc agatgatcgc ccggtgaacc ttgagcacga gaggaatctg tgcgcgaaca
     5221 aaggcgtttt cgatttgacc ggtcacgttc agggaacctc gataggtgcc gaccgccgag
     5281 tgcacgggta ccggcaccgt gatccagatc ggctgggcct ggccggcggg aagatcgatc
     5341 gaatccacct cgagcagcgg atccggataa tcggccgggg gcttgcggag ctggtctttg
     5401 gaaggcgtct gcatgggccg gtcaaccggt acgtatccga caaaccgcgg cttgtcggcc
     5461 tggcggtaga cacccatcgg cgtttccagg gccaaagggg ccaccgatgc ccgcagggcg
     5521 gtcacgggct gctcgctgcg gacgacgatc tggaaggtgg catgctcgcc gcaggcgacc
     5581 tccgccaacg cctcggtgcc ggcggccggc gtcgcgtcgc ggaacacctt gaccagcggg
     5641 tcgaccgccc agatttcaag gcggagcggc tgttgaccta tggtggattg cgaaaaaacc
     5701 acaaggactg ccgccgccag agaaacggat ttgctgagca tggcaactcc tttctgagat
     5761 ctctatgggc tctgtgagga gatagtggta gcgtaaccgg ctcttgtacg atttcaagcc
     5821 atacgagcgg ggccgaggtg caccgaggat attatccggc cacgtattgc taatccgcgg
     5881 attctgacgc aggatatggg gctggatttg tgggtttgag acaagacgag cgtcagaaac
     5941 cgggacgccc cgtacggccg ggggcaagac tcgccgccga gacggacttc cgggagcggg
     6001 aggccccaat tgactgtccc agacgaaaaa ccattgccac cttcgaaggt ccttgtggtg
     6061 gatgacaacg cccagaaccg cgagttgctc tgcgcctaca tggaagaact gcccaacgtg
     6121 accaccattc ccgcggccaa cgggatggaa gccctggccc ttgttgcccg cgagcagccg
     6181 gacctgatcc ttctggacat catgatgccg aagatgtccg ggttcgaggt ctgtcggcac
     6241 ctcaagagcg acccgcagac tcgggatatc cagatcatga tggtcacggc cctgaacgag
     6301 cttggtgatc atgagcgtgc ggccgagtgc ggcacggacg agtttctgag caagccggtg
     6361 aaccgcgtgg agctgacgac acgggtcaag agcctgctgc ggatccgtca cctcaagcag
     6421 gagctgcagc ggacactgga atcgatgggc aataagagtc aggcgtgaca cgaggaagcc
     6481 gtttcgcgac ggagctgatg ccggctgttg gagccaccga tgagcgctga caaggactat
     6541 ctatcgctgc cgttgcggac gttcgtggcg gacctggccg cgaagaggcc gacgccgggc
     6601 ggggggagtg ttgcggcgat ggcaggcctg cttgcggccg cacaggcacg aatggtggtc
     6661 gagtataccg ttggcaaacc caagttcgcc gcctatgaga agaagcttcg ggaggcgctg
     6721 ggggaactgg agcgggccga ggcggcattc gcggaactga tgagcgagga catggccgca
     6781 tacgaacggt tggcggcgtc gtgcaaagcc ggcgacccgg ccgagcagca acaggcagtg
     6841 gccacggcaa cggccgtgcc gatggaaatc acggcgatgg ccggggcggt gctcgcgctg
     6901 ctggatggtc tcaaggaatg cgtcaacccc tacctgctcg gcgatctgaa ggtggcggcg
     6961 attatgagcc tggcggcggc gcagtcagcg gccgtcaatg tggaggtcaa cctccgcgac
     7021 ctggcggatg ggtctgaggt ggagcgagtg aacaaggaga tgactcggat cctcgagaaa
     7081 gcctacgatc atcgcaattc agtgctgaat cagcgggcct cgtaggaaat tcgcctggat
     7141 gatgagaagc ctttgggttt tcgcagtctt ggtcggtgtg gcgtgggcgg ttgccctgtg
     7201 gttcaatcgt cgcggtgtgt gtcgctttga cggaagcggc gatcccaacc gtggcatcgt
     7261 ggtgttcgtt gagccggtcc gctggctgtt cattgtctgg tgcttcaacg cactgtgccg
     7321 ggcgcttcgg cgtgcggggc gaacagatgc cgtgtacttg tatcgctgga gtcgcgcggc
     7381 cggaagtgta ctggttcttc ccgatctgat gcgacggcat cgactcgatg caaaggccgt
     7441 tcgactggcg caattcatcg aacaactgcg ccgtgagcat ccggcatcac cgatccatct
     7501 tgttggatat tccacaggtg tctacatcgc gttcgaatcg ctcagacaga tgtcgccggc
     7561 cacgacaatt ggacaagtcg tcgcgctaca cggcaccgtg tcaccggact acgacatgac
     7621 cgaggtgacc cggcgggcaa aaggtgtgct gaccgtacac ggacgaccag attggctcat
     7681 caacggcctt ggaccgctcc tgttcgggac caacgatcgc gtgcacacgg ccgcctgcgg
     7741 catggtcggc ctgcgaagcg cgccggccaa ggtcgagcag cgtggctggc aaatcagcga
     7801 cgcccgagca ggctacctgg gcgaccattt cacggtcatg tccaggggct ggcttcgggt
     7861 gaacatcatt tcgcgactca catgacgcac aagccaactt cccaacggga catggcttac
     7921 ccttcagcag caacatggct gcctgcaagc cgcttcgtgg ccccgtgaag ccatgtcacc
     7981 ctcgacggca actgtggtca agccgcggga ttggagtggg gcctgtcttc cggccgatga
     8041 gaaaggcgat ggccaccgaa ccgccctatt acctggacat caaaggaatc gaaggcgagc
     8101 cgcctgagcc ggaaggtccg cagcgggagc cgcggcggtg gatcggggtg gaattcgagt
     8161 gctgcggggt ctacacgcgg atctacaaga acagggaagg tacggcctac gaaggcaagt
     8221 gcccgaagtg cctgcggccg gtgcgggtcc gcgtcggacc ggggggcaca tctcatcgga
     8281 tgttcagggc gaggtaggaa gagaagtcct cagttctcag ttttgagttg tcagtcggaa
     8341 aaagcacgca ggggcgcggg gctgcatcat cgcccgggac acacgtacga agcaggcttt
     8401 ccgccatcaa cctgcccgcg cagaacggcc ggtacgatac atgatctatg cgctgtcctg
     8461 cgaagcgttc accgcgaagc agggtcggcc cgctcggcgg aatcccttcc gcattcgacg
     8521 gcaatgagga taggaatcct ccacgtcaaa ggccccagaa aggattctgg ggccagcgag
     8581 cagctgaatt ctggggccag cgagcagcta gattctgggg ccagcgagca gctagattct
     8641 ggggccagcg agccaaaggc cccagaaagg attctggggc cagcgagcag ctagattctg
     8701 gggccagcga agacggcgaa tggtgcaacg ctcatatctt gaaagattgg gccgtcaggt
     8761 ggtccagcca cgcggtggtc actcttcgtc ggttttgagt tcttcgacct cgggaaggtc
     8821 ttcgaggctg gagaggccga agacttcgag aaatcgcttg gtggttccgt agagcatggg
     8881 acggccgacg tcctcggccc ggccgacgat cttgaccagg cccagttcgc gcaggcggtt
     8941 gagcatttcg cccgactgca cgccgcggat ggcttcgatt tcggcccgga ccacgggctg
     9001 tttgtaggcg atgaccgcca acgtttccat ggccgcctgg gagagccgcg aatcctgctt
     9061 gttctggcgc agccgccgaa gccaggtgtt gtactcggca cgtgtgagca tctggtatcc
     9121 gccggcgatc atttcgatgc ggaaggccga gccctgctga tcgtagcgct tgttgagagc
     9181 attgatgtgc tttcggacat cgcgggccga gccgacgccc agaatagaca cgatcctggc
     9241 cagcgtcaga ggcgtgtcgg tggcaaacag aattgcctcg atcacgcgtt cggtggtcag
     9301 ttcctgttcg cctgcccggg gtgcggcgtc ggcgtcatct gacggccccg cctctttgga
     9361 ctcgtcggca tcggcagtct cggccgtctc ctgtgacaac gcggccaagg cctcggccag
     9421 tcggccggcc gtcgggtccg tggcaggctc ctcggagtca ccgaccagcc ggccaccggc
     9481 gattgtcatc agccttccgc cctgctcagc cggctcggct tgcagatcag gggctatctc
     9541 ggtgagcccc gactcgggcc agacgggatc ggaatcggcc ggcaaagcat cggcgatcgc
     9601 ctcggaggcc gcgacatcgg cggggaggcc gtcttcaacg ggctgctggt ctggagtcgg
     9661 ttcacggtct gcactcataa acacacaata taccgttgcc gaacgagatt caccagtccg
     9721 cactgagcac aaaacgaacc ggcgacaggt agcctggaac cagcacccga gggtggcccc
     9781 ctgccggctg cgaagtgtca cgaacgggct ggtgggcgat cgcgaatacc ggctgatgga
     9841 gcaaggagac agggagtagt atgagcatgt gtcggacgcc ggcttggtcg tccggcattc
     9901 ggcaggaaaa aaccatggtt tgggcagcca aaggcaagag tcccttttac cccggccagc
     9961 ccgttccggt tgagttcttc gtcggcagga ccgaacagat cgcccacatc atgcagcgcg
    10021 gagccggcca ggtggccgcg ggaaaacccg tggccatgtt cgttcaaggt gagtacggga
    10081 tcgggaagag ctcaattgcc ggcttcgttc aacgactggc cgagcgggag tacggtctgc
    10141 acgcggttta tgcgcccctg ggatcggcga acttcctcga cgacgtcgga gcaagcattc
    10201 tcgaatccat gttgcgttcc ggagccttcg atcgcagccg gtctgagcgg attcgaaact
    10261 ggctcgccaa gtatgtcggt caacaggagc tttttggcct gacgattcac accgaagcct
    10321 tacggcgcga tgcgcctacc gtggccaagc cgagcggcgt cctcgccttc ctgggcgagg
    10381 ctctgcggcg attgggcgag accggcgtgc gcggcttgtt tctggttctt gacgaaatca
    10441 acggaatcag tgccgctccc aagtttgctc acttcatcaa ggaaaatgtg gatgccaatg
    10501 ccatggcacg cgagccgctg ccgcttctgc tgatgctctg cggtgtggag gagcggcgcc
    10561 gtgacatgat caaatcgcat caacccatcg accggatttt tgatgtggtt gaggttgaac
    10621 gcatgactgt gccggagatg gaaggtttct ttagaaaggc cttcgacagt gtccagatcg
    10681 cagttgagcc tgaggcgatg aagctgctca cggagtactc ggccggtttt cccaagatca
    10741 tgcacatcgt gggcgatgcc gcgttctggc ttgatcagga cggcgtgatc actgaacagg
    10801 aggcgtggcg agccgtgccg aatgctgccg atgaagtcgg caggaaatac gtcgaccagc
    10861 aggtctacag cgtgctgcgc agcagagact accgctcaat cctcggcacg attgcccgca
    10921 ccgggctcga catgggcttc cgtcgagccg acgtagccag aggccttacc gacaccgaga
    10981 agcgcaagtt cgacaacttt cttcagaaga tgaagaggct caacgtcatc cgctcaggag
    11041 accttccagg ggagtacgtt ttcaacgtcc gaatggtccg gctctacatc tggctggaca
    11101 gcctacggaa gaaccatgcc gcacgttgac ctcgcgtcgc catgcccctg attcagtcgc
    11161 tccgctggca ggtattcccc ggtccggcgc agcccaagat ccggtttttt gagaaacctt
    11221 ccgtctattg ccgctgcgga ggtgcctgct attcggccag agcttgcgtc actcgaacat
    11281 cttgagctgg taccgttcga gggcgaattt gccgatctgc tggtcgaccg gcatgggata
    11341 acggccgctg aagcaagccg tgcaatagta gcgaccgggg tgtttcacgc atttcaacat
    11401 gccttcgagg gagaggtagt gcaatgaatc gacaccgagg taatcgcgga tctcggcgac
    11461 ggacctctga tgggcaatca actcctgctg ggtagggaag tcgattccga agtggcaggg
    11521 atgtcgaatg ggcgggctgg ccacgcggag atgaatctcc ttggcccccg cctggcgcag
    11581 ggtaccgatc tttccgcgcg tcgtcgtgcc gcgaacgacc gaatcctcaa cgacgatcaa
    11641 gcggcgtccg cggacgttct ccttgatgac gttcagcttc atcctcacca cgaggtcgcg
    11701 ctgcacctgc tcgggcatga tgaacgagcg gccgacatag tggttggtgg tgtagccgcg
    11761 ttcgtaagga atgccgctgg ctcgggcgta gcccatggcc gcacagcggg cgcagttggg
    11821 cacggggata acggcgtcgg cctcgaccgg tgcctcgcgg gctaattcgg cccccattgc
    11881 ctggcgcacc ttgtggacgt tttcgccaaa gacatcgctg gcggggtcgg cgaagtagat
    11941 ctgctcgaag atgcagtacg ccggtcgaaa agcgtcctcc ggcacgatcc gggaactctc
    12001 catccccttt tccgagatgg tcacgacttc gccgggttcg acctcgcgca ggaacctggc
    12061 gttgatgatg tcgaaggcgc aggtctcgct ggcgacggcg atggcgccgt tttccagtgt
    12121 gcccaagacc aatggccgga agccaaaggg atcgcgggcg gccaccatgc ggtcggggaa
    12181 caggaacagg agcgaatatg ccccttggag gtgtccaagg atgtgcggaa tgacgttggc
    12241 cttatccaga tgggccggtc gggccaggag gtggagaatg acctctgaat cactggtggt
    12301 gttgaaaatg gcgccttgtt cctcgaattc tttgcgaagc aggccggcgt tgatgaggtt
    12361 gccgttgtga gcaaccgcaa cctgtcctcg tgcgtaattg actaagatcg gctggcagtt
    12421 gatgttatgg cacgagccgg tagtcgaata gcgcacatgt ccgatggcgt gggggttacg
    12481 cagttcgtcg aggattcgct tgttgtggaa aaccgtcgtg accagcccga ggttggcgta
    12541 ccgctggatg ggacccccgc cgccgacgct gcagattccg gcagcctcct ggcctcggtg
    12601 ctgctgcgcg tagatgccga ggtacgtcag caggacggca tcggggtgac cgtagacacc
    12661 gaacagccca cagttgtgtt tcaatgaagg ggatgacacg ccgagcgagg ttccttcttt
    12721 ttcaggctaa tcagcgtcaa aagcgcagga tattgaatgc cggtggacaa gtcaagcgac
    12781 cgaccgtcca ataatgcctg cgcatacggt ttgtcgcaaa ggcaaggcgt ttcccggccg
    12841 atggtgcgtt ggcccggaag gagatgacaa atgatggcct caagtccggt cgtcatcatc
    12901 ggaagcatca ccatggatct ggttgttcgg acaccgtaca ttcccgtggg agggcagacg
    12961 gtttccggcc ggggttttac ggccattgcg ggaggcaagg gtgccaacca ggcagtcgcg
    13021 gttgccaggc tgggcgaacc ggccgtgatg atcggccggg ttggcgacga tgcgtttggg
    13081 gcaacgctgc tctgcgacct gaagagcaat ggggtggatt gcacctgggt ccaatcgacg
    13141 ccgggagtcg ccagcggcat taccgtgatc tcggtgtccg acagcggcga gaaggccgtc
    13201 acagtggtgg gaggggccaa tctcctggtc acacccaccg atgtggacgc tgcgcaagag
    13261 gtcatcaaga aggccaggat ctgcctcttg cagttggaaa tccctcttga gactgtgaag
    13321 catgcgattg aggtgtgcag gcggcacggc gtcgagaccg ttctggaaac cgccccggtg
    13381 cccagagacg gcttgccgga ttctctactt caggccgacg tggtttctct gaacgatcaa
    13441 gaagcagcgc aactgaccgg tctctcggcg atcaagagtc ccgcagcaat cgctgccgcc
    13501 ttgtccaaga ggggctgccg atcggtagtg ctcaaactgg ggcagcacgg ggcctatgtg
    13561 ttctcgcccg agggggaatc tgcggtaccg ggttttgcgg tccgtgttgt ggacacaacc
    13621 gcagcgggcg aggctttcac tgccgcactg gcggtggcgc gaagctgggg ctggaagctg
    13681 atcgaagcca ttcggtttgc caacgcggcc ggggccctgg cctgcacgcg gacgggagcc
    13741 caacggtcca tgcctgcgtt gtcggaagtg gaagctttgc tggcaggcca gccgtgacgc
    13801 cagaggcggt catcattgtt ttcggacctt gaggcggcga gacttcgacc tgtctgccgg
    13861 tcgaatccgg gtcgcgtgcg ccccgtcgta tcgtttttgt ggggcttgcg gtctgcacct
    13921 tccgcatggg ttttgccgat tcggcgaaac ctgaggtctg tagcgaccga catatcctct
    13981 taggagacgg ctgcgattcg aagacctgtc ggggattcga ccatgggcaa actcaaagac
    14041 gtgctcacga ccggcgatgt cgccaggatc tgcaatgttg cccctcggac agtatccaag
    14101 tggttcgaca ccgggcagct tcgcggctac cggattccgg gcagcaagga ccgccgcatt
    14161 ccactggccc agctcatccg tttcatgaaa gcccacggca tacctctcga ccacctggag
    14221 accggccgcc cgcgcatact gattgttgac gccgacgtgg atctggcccg gttgctggag
    14281 aaggctctgg aagaacgctt cgactatgaa gtgatggtag cctcatccgc ctttgaggcc
    14341 ggcgcgctga tggagcaatg caagccggcc gtggtgttgg tggatatcga cgtgccgggc
    14401 atcgagggga gggtcctgtt tcggttcatc gcggcccgcg gtgagcttca gggaacccag
    14461 gtggtggcta tgggggccgc cattacggat gcggaccgcc aaagcctgct gcaacagggt
    14521 taccacaaca ccatcgccaa gccgttcaac gttcagcaac tcgctgcttt ggtcgaggaa
    14581 tcgctggcgg tcggaatctg agatccgtgc ttggctctaa ggacggtctt gcacatgtac
    14641 tcacataaga tacaatcgtt cccaagatga gtacatcctc tgcaccggat ccgctgtcct
    14701 cggcattgtt cggcaagacc cgccgggcgg tgctcgcctt actgctgagc catccagacc
    14761 ggtcgttcta tcttcgcgag attgcccggc aggtgggaac gggcttgggg gctgctcaaa
    14821 gggaactgac acgcctggca gcggccggcg tcatcagaag gatctcgcag ggtcaccaga
    14881 ctcacttccg cgccaatccg gagtgtccgg tcttcgccga gttgcggggc atcgttctca
    14941 agacctcagg cgccgccgaa gtcctccgcg aaatgctgtg gccgctaagg gaccggatcc
    15001 gccttgcgtt catctacgga tcaattgccc ggggagagcc ccgagagggc agcgacgttg
    15061 atctctgtgt catcggggac acgacgttct cggcagtggt ggccgccttg ggcggggctc
    15121 acgacttgct gggccgggaa gtgaatccta ccgtctatcc gcccgacgag tttcgtcgca
    15181 ggttggctcg caaggaccac ttcctctatg ctctaagccg tggcccgcga atcgtgattc
    15241 tcggagacga gcgtgagctt gcaggaatgg cagaacaacg gctggctggt ccctcatcag
    15301 acgagcccgc aagagatagc agacctgctc gcagtggtcg accgcgacct cgccgacgca
    15361 caaacgccgc ggctcagcgc tgattggcgg ctgaacatcg cctacaacgc cgccttgcag
    15421 gctgccacgg cggccctcgc tgccgccggg tgccgggcgt cacgagaggc ccatcatttc
    15481 cgtgtcctac agtcgctccg attcacgatt cgcctggagg aaaacgcgat tcgggagctg
    15541 gagcagttcc gcaagaagag gaacatcggc gggtatgagc gggcgggggc cgtgtccgac
    15601 caggaagctg cggaaatgct cctgctggcg cagaagctga ggagagccgt cgtcaattgg
    15661 ctgaaatcgg catatccaca gttggtgccc tccggcgatt gatcccggca ggtccgcgtc
    15721 ctgactgatc gcacgcaagc aggggctcac tttttttctg ctcgcaggtc gtattttccg
    15781 tccagcccga ggtcctgggg ggcgacgttg accggccagt cgccgggttc ggcaaaggtg
    15841 cggcgaacgg cctccagatc cctgaaaccg aattcggcgt tgggctcgat gtatgagcct
    15901 tcgccccact cgttccaagc ttccagcagg gccatccgct cgcgattcat gtcgaggtcg
    15961 accttcagcc ggccgagggc ttccgcgaaa tcccgggttc gacgggcaaa cctgctgttg
    16021 gtgcgcgggc cgtgccaggg acgaggatcc caacccacgg tcaggagcgg cacgtagggc
    16081 gggttcccgg cctgccgcat ggctttccag atcgactcgt ggcccaggag gaatgatcga
    16141 tagctggatt gaggcatcgt ggccccggca ccggagtagt tgtaggcggt catcgagtcg
    16201 aagccggcct tccgaaccat ctctatgcta tgcgaatcgt tcccggtgca ggcggcaata
    16261 tgcagtcctg ccaaaccggc atcgcgaacg cgccgacgca tcgcgtcgaa agccgccttg
    16321 gttttctccg gtccaccaag gtcggtcagc aactcatgga cgctgaagaa gctcacgtag
    16381 ggctttccgt cgcgggtcag gtaattcggt cggcggaagt agtgtttgag ccagtactcg
    16441 gtgacggcca gcaactggtc gggcgtgtgg ccggcgaacg gcgggtgatt ggcccacatg
    16501 accgcgaact tcatgtaatc gcgatagcgg gcgttgagga agccgtcatt gagggcccgg
    16561 cccaattcct ccttgccgtc gcgccagtac cagtcgaaga caaagaatga gatgccgttt
    16621 tcgacggccc atttgacgtg ccagtcggcg acttccggga ggccttcttt gtaccatccg
    16681 agcaccgggt cgcgttcggg aaaatcggcc tgtttctgcc agcggctgat ttgatccggg
    16741 gaccaaccgg ggaagtaata aacgccgatc tggtagcgtg tcttcaccgg cctcggcttg
    16801 ggaacgtcat agttgtcgga cccgcgatcc agaagcgtcg gatctgtgat cggctcgtcg
    16861 acatgcagga tgcgagtgaa cgtctggcca ttggcgtgca aggtaacgct ggtcgatccg
    16921 ggcttcgacg ggataatctc agtggcgacc tcggcgcgct tgccgggttc gacggccggc
    16981 atgctcacag aaggcttctc acagcggacg ttctcatcga ccgcgaacga cgccacgccg
    17041 gcagggaccg ccgggccgcc cacgtgttcc agcacggctt gaacgccggc ggccttgccc
    17101 ggtctactaa ccggacgttc gaaacccatg tggcgcagcc gaaggaatgg acgtgtcgca
    17161 tcgttctcgt cgatactcat cgatcgcaac gtaagggtct cgcttccgtg cgtgccgaaa
    17221 gccagagcga gatgagtcac cgtccccttc cactccggga agcagcggag atccagttcg
    17281 attgaacgag catggccttc accagcgatg gggatggggc ggccgatcag gccgggctgg
    17341 tcatcggtcg cccagtaaaa gcccagcacg tcctcgccgg ggcatcgggc gtccaaccga
    17401 aggatgaatc gccgttccgc ggcaaaaggc gcgactacga ccgcaatgct cgcctgcgga
    17461 gcggtcgccc gcacaacgag gccctcaccg gtccgctcag ccgtggaggc gcacatgggc
    17521 cgccacgggt cgttcgcgtc gcgaaatgac catgccgggg ggcctccgga ctgcgatgca
    17581 tccacgatcc gaatggacga caagcggcat cggacgcctg aggggggatc gaagcggatc
    17641 cggatcacct tttgcaggac gccccagaag ggccagatgg tcaagctctg tcgtcccccg
    17701 gacggcacga acgccaagtt gacccatttg ggctcgaacc cgccgtaggg gccggttgtc
    17761 ttgtttgtga agaagagttc agcccggcct gcggccgaac agtccagatc gatcgtcacg
    17821 tactgccggt tggtggccgg cgacaactcg aactcgggac tcacgatgat ggggtcggga
    17881 ccaagggcat cgaaggctac gccgtcgggc ccgaattcaa cgttcttgat agtgcccccg
    17941 ggcaaccaac cgttccatcc tgatcgggag tcgaaatccc aacgggccag gactctttcc
    18001 gtcgccccgc gagcggggaa gacgactacc agaaagaaca cgcccagcag accggcagcg
    18061 gaagatcgcc tatgtttgcc catgacgcca gctcccgtga attcgtccca tgaaccgcat
    18121 tgtgcccgca tctcatgcga ggtcggaatg ggttacccgg atcagtggtc ccattgtaca
    18181 acggagaccg agaggaaagg atgggaagaa gaaaggcagt ccggcattga cggacgaccc
    18241 gcgccggttg tttgaagggg ttacagaaat accataccgg cccgccagcg agggccggac
    18301 tccgttgcca cgtgaacgat gcggccgacg cgacgcacgg gcggacgtcc cttggcaggc
    18361 acttcgagct cgagcacttc gtcgacttcg atcggcaacg ccattgagat cctggctccg
    18421 ccctggctga tgtccaggac aatcacctcc gcccacatgt ctcccttgcc cgggtgttcg
    18481 gtacgtttgt ccagccatcg agcgcgggca ttcaggcagc attcgaatcg tggatggcgc
    18541 cttcgattga tgggctcggc gcgcgtacca cccgacggcg actgctgaac aaatgagtcc
    18601 aacgaaaggg actcatttgc ggcgccctga gggggtactc gctgcgggtc ggcctccaga
    18661 aggccgcgaa cgatggcgtc ccaggcatcg accatctccg gatcgaagca ggtgctcttg
    18721 ccttccgcaa gtaccttgag cgtctgttcg ggggggagcg gcccgcgata gggcctcgcc
    18781 gcgcaaatgg catcgaagac gtcgatgacc gaacagattc gagcctcttc gctgatctgt
    18841 tcgccggcca gaccgatcgg gtaccccgcc ccgtcaaaac gctcgtgatg ggagtgtacc
    18901 atgtccataa cgatctggga catggcaggg tttgtcttga tgaggtcaaa gcccgtaccg
    18961 gggtgagact tgaccagttc ccactcttcg ggtgagagct ttccctcctt gttaagtatc
    19021 tccgtgggga ttctgcgttt gcctacgtgg tgcaggagtc cgccttcgat caacgccgcg
    19081 tgccactgcg aatcgtccgg tttgagctgc ttggcgagca agccgcagcc cacgccaacg
    19141 ttaaccatgt gggtgacggt atagtaatcg tgcgtcgcca tgtccatcag gtaggagaag
    19201 cccacctgat cttcgtcgac ccactcggcg aactggcggc tgacgtcttt tacggtttcg
    19261 agggactccg gccgctccgg aaagagcagc acgtcgtcaa tcattttggt gcatgcctcc
    19321 cggacgatgc ggattcgctc ggatcgttcc ctatggggat cggtgtaggt cttctcgagg
    19381 tagcgggcga gagctttgcg atactctttg tgctgcgaca tcggaacgta caggaaccgg
    19441 acgttccgcg ggatcagctt ctctcgcagc ttgctggtaa aggcggcacc cttcgcgcag
    19501 tagagcacat agctgctacc atctccggcc gagtcgaggt atacgtccat ctcgagggtg
    19561 ccgtcctcga tggtggcaac ctcgaccgga aaaaacctca tggcctcgct gccgacggcg
    19621 aggtcggatt ggtcgaaggc agtcgttgtc atctgacccc cacaagttct ccctggtgca
    19681 gggcaaacgt ggccgtatac gtgtagatcg ggctataatg ggggtctcat gatatgtaaa
    19741 tgcttccagc cgatgtgtta aaacgcgcaa ccgcgtccac ggacatggca ccaacgcaag
    19801 acgccgcact caagggaata cctaccgcgc ttacagccgc aagctaagag aaagactgca
    19861 cccgcggaac cgataaccgg ccctgccgag tccggtaaca gaaaggtccg cggaggacct
    19921 atactggtag gtgtgcaacc cgcattttgc cagtgtccgg ttggaaggct gtcccacata
    19981 gcttcctgag acaggcggta ataccttccg ggcatgcgcg gaggtttggc actcgaccgg
    20041 gccagaacgc gatccgcact tggccgggtg ctgatcaatg cgagcatacg gctgatgctt
    20101 gaacaatcgc ggccggcagt catccgtccg gacgcgggca tcctggcccc ggcctgaggg
    20161 gaaggaaaag tgacttggcg gtttccggaa cagcaagccc caagggaagt cggccggctt
    20221 gcggggcgcc agggcgcagg ggttgtgcaa aggcggaacc cgtgctcgcc ggtgagtttt
    20281 gaacgaatcg tctaccgccg ctccgggagg ggcagcccaa gtcgtgccat gaccatctgg
    20341 aggtcgctcc aggcttgcgg cttctttcct ggatctcgca aaagataggc ggggtgaaag
    20401 gtgggcatca acggcgtggc cggcccgatc ataggtgatc cggacaagaa gaactcatgc
    20461 caatggcctc ggagccggcc aattccctcg gtggttccca gcagggtctg cgtcgcgggc
    20521 ttgccgagag ctacaatgac ctccggcgtg ataatgcgaa gctgctcttc gaggtagggc
    20581 caacaggcgg atatctcgtc aggagcaggc gtgcggttct ccggcggacg gcacttgacg
    20641 atgttgcaga taaacacctg gtctcgggtg agtcccatcg cctcaatcat tctcgtgagc
    20701 aactggcctg cccggccaac aaaggcgagc ccctgcgcat cttcatcggc ccccgggcct
    20761 tcgcccacga agacgaggcg cgcagccgga ttcccctgtc cgaaaacggt ctgcgttcgc
    20821 gtccgcgata accggcactt catacagcct ttgacttgcc ctgcgtccag ggtcttgagg
    20881 gcctcctgcc gacggagcaa ttcctccggg gaaagggctt gaagggccac aaccggcttg
    20941 tcccgtcctg gggaagccgc ggcaggaggg tgtttacctg aaagctgtgc gtgccgtggc
    21001 ggacgtgggg acaggggtgc ggaatcgatc cccagaagcc gatcaatctc cagatatcgt
    21061 tccaaggaac cacgaatctg atcgggagga agccccatgt cgccctcgtg tcggattgcc
    21121 cgcgcgaccg cctctttgcc aggataccgc tcaggcaggc gttctttctc cggcgaagac
    21181 ggccatacgt gacaagtgga cggtgtttta agcccccgca aaggcgtcgt caatcggcag
    21241 gcaggtgtca accgaaagag tgtccgagga ggtcgacgat taaaggcaaa aatgcggaaa
    21301 aacggcttgt tcaggcggcg cagatatgct agcatgctgt gcagcaggcg gttgtggcgg
    21361 tgccgagcat gcaactctgg gctgccacca tgcacgcccc gctcaggggc gagccggatg
    21421 cctgtttcca gtccgccggt taccggccgg catgattaag gagtattgcc cgtgtcaacg
    21481 tcagggcggc gtggacggct ctgattgctg acggcaatcg tggagactgt gggatggcgt
    21541 tcgattcgaa caggtacctt gcggtaggtt ccaaggtcag agtagccgaa ccgatggaag
    21601 tacccgactg gtcgacgtgg gatgatgacc acggccgcat ttcatcacag atcaagaagc
    21661 gccttcagac gctgtttttc aaaggcaatg acaagatcac ggcggaagtg gtgtacatcg
    21721 ccaaggagtc agagcgagag aagctgcgcc ggctggggcg tatcaaactg cggctgcgag
    21781 accagagcgg ttgcgcactg gtgatcacgg ccgatcccaa gcatcttgaa agcgcgtgac
    21841 ttgctccggt ccgcccggcg gttaccatca cctggttatg agcgacgccg gcgtagtact
    21901 cattcctcac agtctcaccg aagcacggct gtacgtaaag acgcggcggt gcgcgtcgtg
    21961 cggagagggg ccgctgctga tttctcgtga agcgccggtc tatgatgccg ccgcacacgg
    22021 gttgacggtg accgtggtgt gtgacgaatg cggccaaccg gcaagcatca ggttctgcgt
    22081 ggcagagctt gagcgccgcg gcccggtcct gagcatgctc gacgaactgc gggacgtttc
    22141 gcaacaaccg gccgcccagg tcatcaatcc gacacgccgc ccgtcgcgtc tgatcgacgt
    22201 tgccggctgg gtgatgctgt acaccatgct gaccgaggct gcgcgaacgg aaagcgtcaa
    22261 atcgacaagc attgaaagac gggcggccgt acggcggatg cgaatcgagg ccgccctgtg
    22321 tctcgacgag gcactcaagt tcttcgacga ggatagtgat ctgccgcccc gggaggcttt
    22381 cttcacgaag gagagctatg ggcgttttct cgatcgaccc gagttataca cccggcagcg
    22441 gcttatcgag atgcgtggtc agtcgccgcg atgagccatc cggcctcgaa gcacggcaag
    22501 ctgccgggct ggtatctgct cgactgcggc acgagcaagc ccgtgccgct gatccggttc
    22561 cggctcggtt tgccgaaggc gacgggcccc atgctgctac gataagggga ttcgcagccg
    22621 cgttacggag gccggaggca aggtgacgac cagtgagccg tcgggtttcg ggcggtcgag
    22681 aggcagatct cgcgggataa cctccgccgg gctctcgaac gtgttggcgg cgttgagctt
    22741 gctgtgagtg agcacggtgc cggcaagctc ggccggcctg gcgctaccca ccagcttgat
    22801 cgcgacttcg atcggctcgg atgcgtgtgt gtgcgtgagc gtcagagtca gttgcctgtc
    22861 ccgcacggac gcggagcccg ctacgcgaaa gatcttgcga tcctttccat cgagcgagaa
    22921 ggtgacgggg tcggcggcga cgcgaagcgg gacggcggtg ccgttttgat ggtcgaggta
    22981 catgctgaac acgtggtagt tggtcgtctc gatgaacttg tcatcggtgg ccaagtagag
    23041 cgactgaagg cagttgacga gttgagcaac ggcacatagc gagagcttct cggcgtggcg
    23101 gtggaaaatg tcgagcgtca gaccggcgac gagcgcatcg cggagcgtgc tgatctggca
    23161 gaagtcaaac tccttcttga tctcggtccc cgacgggtgc cagcagcccc actcgtcgac
    23221 aatgaacttg atctcgtgct tggggtcgaa ctcgcccatg gccttccagt gctcgttgat
    23281 gaacgtctcc atgtagttgg ccttgtcgag caggtcgtac cactgggcgg cgttgaactg
    23341 agtggcggtg cccgaacttc cgcagtagta gtgaccggcg aatccgctga tcggggcggt
    23401 tgcggcgtcg cgccacttct tgaggaatcg ccgggtccac gagacgtcgc cgccgttggg
    23461 tccgcaggcg accaagtaga gcggcacgcc gtacttcggc acccaggtgg tgaacttgcg
    23521 gtactcggtg cagtagtcct cgggcgtgta ggttccgccg cagccccagt tctcgttgcc
    23581 cacgccccag aagcggacct tgaacggttc gtggtcgccg ttggcgttcc gctcatcggc
    23641 cagcgtggtg ctgccggccg gagcgttaca gtactcgacc cattgttgga actcctcggc
    23701 cgtccccgta ccgacattgg cggccaggta gggttcggct ccgaccagcc ggcagaactt
    23761 gatgaattcg tgtgtgccga aggcgttggg ctcgccgcgc tccccccagc gattgaagcg
    23821 ccgcgggcga ccggcggccg ggccgatgcc gtcgcgccaa tgatatcggt cggcaaagca
    23881 gccgccgggc cagcggatca ccggcggagc gatcttcttg aagctctcga ccagagcctt
    23941 gcggatgccg tccacgttgg gcactctgga gtcgggcccg acccagatgc cgtcgtagat
    24001 aaccccgccg atgtgctcgg tgaaatggcc gtggagcagc ggactgatca cggccggctt
    24061 gccatcgggc tcccgaatct gttcgtcgag cagaatatcg agcgtcgccc gcgaatcctt
    24121 gctctctgcc gcgaacaggc gagcccgcga accggcgccc aatgcggccg ctgcgccgca
    24181 ggccaggctc ttcaggtaga tcctccggct gattcgcgcc ttcatgatgt gaccctcaat
    24241 gctaccggtg ctcgcccgat gcgatccaag acgccagtgg tttaaggctc gcgctccctt
    24301 ctcaccatgg cacccgtcaa ggcggcagca ggttagtcgg caatcgtgta atgcccgccg
    24361 gcgaagtaga ctccggcagc aaccagccgc ttaagttgcg gcgagttctg ctgaaaggcg
    24421 ctcttaccgc tggcgatcca cacgatcttc cgtctgtagt tgcggagtct gtttccctcg
    24481 ccatcaagcc accccttgag ccaagtcagc ttatctaaga tttcacgaac cgcacccgcc
    24541 ccgctggccg aatggacctc gatccagtac aggaattcgg atttctcctt ggtcgcccac
    24601 ccgacaacgt agtcccatcg gttctcattt gggcgcagat tctcaagagc tgcatcgata
    24661 tccgcgctcc cggccagtct ccttgtcgct attgcatcga ccttgttctg atcggcagga
    24721 cggagcgcct gcttgcccgg tcggtaagcg tgttcgagtc ccgcagtctg ttcaacatcg
    24781 ttttggaagc tcataacgga aagagcgtcc ccgtattcgc catggcggtc gcgaccgcct
    24841 cgttcgcccg accgctgaag tcgagcatcc caccccaact cgactcggtc ttgtccagcg
    24901 aggcagggtc caagccggtg atatcgtgga cttgaccact gtcaccatcg aagaaataca
    24961 ctttgcattc cttcctgagc gcgttggctg caacggtgcg caggccttgc gtattcggcg
    25021 cctcgaacac ctgcagcagc ttctcgggtt ttgcctgcgt cctcccaagc ttctgaatca
    25081 cccacacgag atcaagtacc tgcgggacat gagtggagat gcagactcga tagccgcggt
    25141 ccagcaactc gagcacgagc agcaataccg ctgtgatggc ccgcggatgc atgcccatct
    25201 ccggctcctc gatgatcacc cattgaacat cgttgcggcg ggacactttt gccgggggca
    25261 taagccaata cagacccagc aacaagggca caaactctct ctgccccgcc gaccaaacca
    25321 tgtaaggcag gggggcggtc cccgccgtct ggccaagaac gagccgtttt tgcgacctga
    25381 gtgcgtccac cttgagacga aactgctgga agatcgtctt ctccagcagc ttgcggtact
    25441 cgcttttcag ccggttgctc tttggaaaga ccgcgtctcc ggcaccaagt tcttgttcca
    25501 tgagaagtcg aagcttctcg ctgaacgatc gcaccgcgaa gggatcgcca gcagagaaat
    25561 cactgaaagg acgaggccag ccgtcacgaa gcgtcagcac gcgctgcgcc ggaaccagaa
    25621 agacggactc cttcttgctg accgcctttc gactgatctt cttgttcgca tcccatggtt
    25681 gatggtccca gagcacttca cttagacctt cctgccagac cgtgtgcatg ccctcgccga
    25741 aatagagatc aaggaacttc gagacactct ttccccaatc caagccgtat tgggacagtt
    25801 gagcctgaac gaggcctgtg tccagaacca gtttcagcca ttgcaaggcg atgctcttgc
    25861 cgctggcctg tgggccgaca aggaccgtca gatcgccgaa agagagattc gcctcttgaa
    25921 tctgaccgat gtttcgcaat tctaacgacg gaggcatgtg ctgatcctct cagactgtcg
    25981 tgaagttgat gcgagcaaca ataagccgaa catcatgatg gactgcaaca agaccctgtg
    26041 cgggaccggc atccgtcttg ccggtccagt cgaccggctg gaagccggcc tgacaagctc
    26101 cgtcagagtg ccttggtccc attctcgtga tcggcctcgt actggaacct agaaacgctg
    26161 caaccgccgc ccgtccagta ttgtaccgct gatcggcaaa gtcaccaagg atgtttgaat
    26221 ggtttgcatg gacacaggac ggttcgtcat tccttttgtt gttcgataga atcggagtaa
    26281 tgacagaacc acgacggacg cgagtcgcac aatgaaccgt gcgctatcga agtgagcagc
    26341 gggccagaac tgatttcgac tcacacaaca atgacgacga ggatcactga tgaaagaaaa
    26401 gctgtttgtg ggctcctggc tgttggcaca agtccttgtc acaggcatgg ctctgggcgc
    26461 agaaagctgg acggcgccga cggtgcggct tccggctgcc gagcggcacc ccgtgattgc
    26521 ctgcgcaccc gaggagttgg cccggctccg cgccgcctac caggcgggcg ggcccgcaca
    26581 tgttgtcgcc gccaacatcg tcaaggcgac cgacaagttt ctggacgacc cggtcacatt
    26641 tccgccgcgg ggcgggcagc acaaccagtg gtatcagtgc gacaagtgcg aactgggcct
    26701 caagacgctc agcgacacgc agcatcaatg ccccaagtgc ggcaagatct ataccggaga
    26761 gccctacgat gacgtggtct tcaagcggca tcacagccga aacctcagcc agatgcgtca
    26821 ggcggcttgg gcctatgcca tcaccggcga tcgcaagtac gcggactatg tcgtgaagat
    26881 cctgctcggc tatgccgacc gctatctcaa gtacccctat cacaccaata gcgcaacgaa
    26941 gcaacccaag gggctctcgg gcggacacat cgacgaacag acgcttggcg aggcgaccgc
    27001 cctgtcccgg cagatcgctc cggcctatga cttgattcac gattctccgg ccttatccga
    27061 cgccgatcgc aaagccgtcg aggagcaact catcctgccg atgctgaaga acatcgacaa
    27121 gcacaaggcc ggcaagagca actggcagac gtggcacaac gcggccatgc tctggggcgg
    27181 cgcggtgatc ggcgacgaag cctggatccg caaagccatc gagcagccgg gtcagggctt
    27241 cgcctttcag atgagccact gcctgtccga ggaaggcatg tggtacgaga acagttgggg
    27301 ctatcacttc tacacgctgg atgcgctggt ggaaacctgc gagggtgccc gtcggctggg
    27361 aatcgatctg tggagccatc cgaatctcaa gaagatgttc ctgctgccga tccgctacgc
    27421 catggccaac ggttcgctcc cgcgattcgg ggacgatgtc aacgcctcca cacaaaggat
    27481 cggctcactc ggtgaaccag cttacctggt ctatcgcgag ccgctgctgg tcccctatct
    27541 gtcaaagaca cccacgcctg agaacatcat gctgggccgc aagatcgaga ggcaggcgct
    27601 tgagcacaaa ccgtctccta ttagtccctg atcaagcata ctccggcgag gttttctcgc
    27661 tttgttcgta aaaaag
//
LOCUS       DAOTPW010000039        27668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1800_length_27668_cov_5.308876, whole genome shotgun sequence.
ACCESSION   DAOTPW010000039 DAOTPW010000000
VERSION     DAOTPW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27668
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1800_length_27668_cov_5.308876"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="P5316_05390"
     CDS             <1..250
                     /locus_tag="P5316_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84425.1"
                     /translation="MVLRVTSSGRGMIAPVVSCAELPGETPAGQPAVFDVRAGGGASA
                     SRSNSAAGCGLIGVAPAFAFPGFYLLHRHRRRGAGRVR"
     gene            complement(279..2219)
                     /locus_tag="P5316_05395"
     CDS             complement(279..2219)
                     /locus_tag="P5316_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR84426.1"
                     /translation="MMTRVYATRPAGPISVLCMALLTAATEAQWSNAQLFNLPGEAGL
                     DVKTAKIAAARSGGFHGIYHVNNRIRYRRYSGGTLTSPIDVFPGTHFMANGAIAEALN
                     GDIHVVFEDWAWGEKNDKPEVRWYKSSNGGASFPFTQVLSATGGCAKHPHITAFGTGT
                     SAEVIMSYFRSGSTGGCDKSLYFARYNGSTWTAEADIGSHSNSEYDCFGMARSPLDGS
                     VYRSYDPNGSSMAMRRYSGSWGPQINLNSGAWPVRQHMAISATGQVMLLWDQDERIKS
                     QLYTPAVGPGPVIDVSAGGYAGSCDVCWVPGTNDFYMVVARPTGGGGFHVYGRRWTAG
                     AWSGEESVMNGQTDAFTVTPVVAADDTGALYCIWEYWGSDKPQQWYAVKPSSLPPGPK
                     GTISGFVRDNLGAGLPNATVIVAGVPSAVSQSNGAYTLLVPVGTHSAQASKTFYTTQT
                     VSNVTVLENQTTTVDFMLLTQPPTPVASLTVTPGNAQNQLNWTNSTSVNCNGVVIRYS
                     TTGHPAGPADGQLLLDRSASPGATDSFLHTGLVNGTRVYYSAFAYFQDASRFYSTTAT
                     TASGSPYGVADFDRDGDVDQADFGYFQECFSGAFVPQNDPKCQGAKLDPYDDDVDMDD
                     FAIFLQCFAGPKIPTDPDCANP"
     gene            complement(2237..4162)
                     /locus_tag="P5316_05400"
     CDS             complement(2237..4162)
                     /locus_tag="P5316_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR84427.1"
                     /translation="MRRIRSTTAVAGLWLLCSAGSLCAWTTPGPVVSTSEDSLDPRMT
                     VDSNGRCHVVWRERTGGSLFRIWYTNNTAGPFAGPTEISQTGPAQSYSPVVAADGADL
                     HTAWICDPDGTNIEIWYRKMTADVWGPILNASNTPIKSLRPAIAARNGIGPVVAWDEA
                     IYADDNYDTYFADWNGSGFNPAINISNTPGGAVYGSVNVNIAVAPNGDVTAVWADRIS
                     GSYHVNARRRVGGVWQPRQEISTLQTGPATPGIAVSGDNRVHVVYEAEGAIRYQVWNG
                     SAWTAPATLPGGLNSGIRPKIAVDSAGIAHVAADAFTDAQNNRDIFYTSNESGSWGNW
                     SNISNTAGTASLNADIGYAAGLITVTWQENSNGAGGTDVYNTWFSTQPRPLPGPTGQI
                     TGTVRDSFGHLLSGAAVSAAGYYYVAITAADGSYVIPTVPVGTYNVTASKPWHTSQTL
                     SGISVTAGSTAIADFSLVPTPPEPVTSFTATAGNTLNTLSWVNPAAGNFAGTMIRFST
                     TAYPAAPDDGSLLIDRPAMPGTTDTYPHTGLTNGVTYYYTAFAHSDTYVYSPGATAAG
                     RPFGPADFDRDGDVDQQDFGHFQQCFSGAFVPQPDLNCADCLFDADDDVDVDDFNMFM
                     GCFSGPGVAADPYCMVP"
     gene            complement(4191..5906)
                     /locus_tag="P5316_05405"
     CDS             complement(4191..5906)
                     /locus_tag="P5316_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84428.1"
                     /translation="MHLLRTVSGILGIGLLLPAAAHAWTPEYSIHPGITWPGGKPSMG
                     MDASGRIHLVYSPTVPPATAGHIYYRFLNGTTWTTPQDLPGPDRKEPESDMCVSPDGH
                     VYVVGIARVDNTTATPYTVYFWEFDGTTWAGPTMLSSGQGNDGNNCTSPCIARDRNGD
                     LHVVWSQDGLTGGEADIMYRKRQAGVWQPIQNITRNNAGTSYGSCDPDIAVDADGNTV
                     HVVWHDDFLNNGFQAYYTKNTNLGDPAAWLPSIEWYQLSTGNYGKAPKIFLDHNDLPN
                     VFWQDRFGGSENRQGYSRWTGSTWTAPANWGIDFVQDAIFDASNTMHAVYAQASGSTE
                     IYYRQYQYSGATYSEMISTGPNTLKAYTAGIVMDLAGKLHVVWCERKSVDGNEQRYVF
                     YSTTAVLGSPDPVSSFTAQSLDGAVALSWTNPGSMSYRGTTVWSNPDGYPASPEDGVL
                     VCDRPAAIGSSDSFAHTGLTNGQRMYYAAFTYNANREYSVGVNLAAMALSPVDFDRDG
                     DIDQEDFGLFQTCLSGAFVPPTEPACQTMNLDGDLDVDADDLSLFLGCMAGTGLPPAV
                     NCTNQ"
     gene            complement(6440..9763)
                     /locus_tag="P5316_05410"
     CDS             complement(6440..9763)
                     /locus_tag="P5316_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84429.1"
                     /translation="MVRMAPLHRFAVLVVLVSPLAAGATVGPEQAVVGEPVDLARFAT
                     RLTDDHGIGVEWDNPREVSEIALSGVDDSLLGSLCVEWWGSVWPGNGTGGWMRLDDPF
                     NGRWVRVKATVEKDPRRGLVFRLPPLVKEEWDQEPNPAWKEPPAYRRTLKVRVVAEGR
                     GLPSDAVLSAYGQSRWREAQFDIESRHATDAIITRQLDVINGVVLEMKSLAPPRSAEI
                     EGRKWTAQKEAGGSSGVRVRLRYTDNRDPNSNDLTRVTVRFGSDTWSTGFSFVPQDVL
                     ADGAMRLPDFGTLVTSSSSGLTLANDPGPPTGNWRRPVRQRLTEKPEMTREMAMAGIP
                     RLKPAPWVPLGVPSARQEFFVSPAGDWSIWAMSLNNDNGRDAKRWSFQSDFGTQRLQQ
                     RLDYLLDTREQPAFDGRDREGCIRRLEQGHLPLIHAEWYTGPMQFRHSLVTTTLLGDY
                     GDDVTRKGDETVVLLGRLAVTNTDGQTRTATLNLRVHDKSPISLDPDGVMRIERPSNR
                     PVPEGLTALRGVISNDKPSGGGITGWTVQRGDEPEDHPILRWQAELRPGETRLMYFKT
                     PFVELLEPQELTRIREISYEVEAPAIMKYWQTRLGRGMTLDVPDTAIMDFYRANLWHN
                     IITTDRDPETGLYNQGVGTVGYRVFANETVMIARSMDMRGEHVEAERFIEPMLRFQGH
                     EPLKGRFSTKDGVFHSAGAYTHGEYAMNHGFTMWGIADHYLMTRDRRYLDRVAPQLIK
                     GCDFLINERKSTMGKQGSPRPSIHGMAPACSLEDVVEYQYWFATNAYFHLGMKRVAQA
                     LADAGHPEAERIAVEAEEYRNDLERNMREATTRAAAVRLRDGHFIPYTPSRVFHWRHL
                     TEGWIREALYPAIHLATAEVVSPNDPLITWMLDDLEDNIYFSWQSGFNIGDFEQTWFE
                     HGGVTLQPCLVDSATVYMARDEIPAALRAFWNTYALSIFPDVNCFSEWAKALGKPGGP
                     LYKTSDESRFIMWLRQLMIWEDGDRLWYGRAVPKQWLENQKDIRIERAPTIFGTAGLV
                     IHSESDSGRITATIRIPTRTPPKEVWLRLRHPADLKPTEVFVNDRPIAPGNLLGQDIR
                     LVPGIEDLSTPVEVTAVFR"
     gene            complement(9791..13456)
                     /locus_tag="P5316_05415"
     CDS             complement(9791..13456)
                     /locus_tag="P5316_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR84430.1"
                     /translation="MSAREIQMSALTLVFLQAAIAPAAEKNLPPTITGKDGSVMLVVP
                     AGPFIMGSAEVWDETPPHQVDLAAFYVDRTEVTVAQYARFVKETGITPPPDWINGIPP
                     AGRDNMPITNITWFDAMRYAVWAGKRLPTEAEWEKAARGTDGRRFPWGNVDDETLRNL
                     GSDRIASTSREATGGTGPPKGASPYGCLDMSGNAWEWTADWYDGYPGTSARSLHFGRQ
                     YKVMRGGGGIYFYGTANAGTCTQRARLVPYGAHDSLGFRCVLDADPAKRPYDPQVVLK
                     EAEERLKTIRNRPAKLSYEIEFERMKQAARVPVTVVGRSGQRGHVRAGFPLPKGAVRK
                     ETDIQVFGPVGNNIASMATALSKWEDDSIRWALLDFTGEAGQVFEIDISGRPGGSNAE
                     HESVQINAAGKAAAMRTDKITVAASPEGLIDQIRLRESDVVLGKLTMEMQVTLEGEAT
                     TLSAMPAQQIEVEEARALHGCLRMQGFFGTADGRQTAMKYDLRAQAAAGSHRLTLMLT
                     ITHWADRKKSQNRDDPYGDPDPAINVADLSVRFALAGNPALVALGTDGGMLAADGTVE
                     LQQPDDLRYVIRQTGKPDTNGTRAPGWTAVKQGDTWCVLGVRHFWQNCPKALFVTPEA
                     IGARLYTGSRPFNWEAGLAKTHEIILDFPLAKDDSAPPNVVLDPLRMTMPPMWACGTK
                     AFGAVLPRSTEALQSFPYWECWREADMRDYVNRMPFGMRDFGDGYMGGPYKGKNAYQN
                     LEYDVPYNYLLEFLRTGDPWYLDAAEPMARHQTDVDVNNFNGMVWKHSPLHTATEADL
                     GHVFLRGVLLHYLLTGETRQLEMARKVGDFVAAMLTRESGSVVGNERQIGWSLYLLTG
                     MYEVTRDEKYLKACESLCNSLLAGQAATGKFAIRWDNRIAFFNGIAMNGMLSVQENNG
                     DDKLADGILRVADRTLGFYPEYACRTLNAFSWALQQTEDPRYLDALERCWQSSIEFLH
                     DRNVASEATHMWRFPRFAARYGLFRMFDEATVLPEPSSWKATRFKAPDVEVFLHVSDR
                     PAPILVIREGLAEGRITLFDVAGNTLRQVLLKDAGNYFEPAVLNLPGNRGLFRMRLTG
                     GDAFGWQVHRDRAARMTVVDVAGRHMPQMLPRAVGFVREGAKEVKIRFEAMGEGFHTA
                     TLYNPAGRPVATVRHFIDFQDPGRYELELKTVASGDTRGWALEVCNLKVLSIDGLLPY
                     WADDEVELFNPERFGMH"
     gene            complement(13788..14672)
                     /locus_tag="P5316_05420"
     CDS             complement(13788..14672)
                     /locus_tag="P5316_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR84431.1"
                     /translation="MTHRNEPTRVVRSVSTGFTLIEVLVVVAIIALLIAILLPSLSRA
                     REHARRIQCASNARQIAIACTFQSEDTPAKVYASTASTGSDSLNHIFPKYLKGVQTAL
                     CPGTRNVVRENRRSFNTFYDRIILDDLEHSAANAADDTGGHSYEIWGWFDGPSIYLDR
                     TKIDGRSESVGQQLCLPRKPAVEPLYSQRTGCLVKKQSTIKRPHTCLLVLDSDQGGSG
                     QPGDENNNYPDPANNHGKDGLNVSFVDGHTQFVRPSQVTSIYLQSYNDPPYDNWRTIA
                     PWLRETQQNGYTVWTRVE"
     gene            15424..15603
                     /locus_tag="P5316_05425"
     CDS             15424..15603
                     /locus_tag="P5316_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84432.1"
                     /translation="MMNSPISAEETTDSHRPERGRRQTYSDHLHRGDFAGRERADSLV
                     GDIFKTILLVVESAA"
     gene            15795..17672
                     /locus_tag="P5316_05430"
     CDS             15795..17672
                     /locus_tag="P5316_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HRR84433.1"
                     /translation="MKLHALDITIIFVYLLVVSLIGVLLRRRAARNLNSYLLGGKELP
                     WYFLGLSNASGMFDISGTMWLVTIMFVYGLKSIWLPWLWPTFNQIFLMVYLSAWLRRS
                     NVTTGAQWIGTRFGRDMGSELSHAVVVIFAIIGCLGFLAYGFVGLGKFIQIFIPWEII
                     SPYVPFNVPAQYVPHVYGIAFTLVAVLYTILGGMTSIVLADIIQYVIMTVSALTIGGI
                     AMFHLSSQALNVPPGWHSPFFGWKLDLDWSGIIADVNEKIAADQFSFFGIIMMLMLFK
                     GIMASAAGPAPNYDMQKILATRNPREAALMSGFVSVVLMPIRYFMVAGFAILGLLYYH
                     RLNLVVGHRIDFEQILPSAISEFVPVGLMGCLLAGLLAAFLGTFAGTLNAAQAYLVND
                     LYLKYIKPDAGNRRVVMVNYATGLVVVVVSTVLGFFAQDVNSLLQWIVSALYGGYVSS
                     NVLKWYWWRFNGHGYFWGMIAGLVPALVLPLVFRQTLELYYFPLLFVLSMAGCIIGTL
                     ASRPTDEETLVSFYRRVRPWGFWKPIHEKVVAADPGFRKNTDFGRDMFNVIVGTIWQT
                     ALVLLPICVILLKGRSVLIVAGVIVLTSWILKKSWYDRLEASGAAAPVEASRGSGARL
                     A"
     gene            17749..19707
                     /locus_tag="P5316_05435"
     CDS             17749..19707
                     /locus_tag="P5316_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84434.1"
                     /translation="MSSIVGVVLGKSVMHRRASRRRYSSRGRASPTERGAVGGPAWLL
                     AMAAGLCLAGCTPLPASKAPSMNDFVRREGDRLMDGSNEFRFISVNIPNLHYVEDDMR
                     FSRSMPFRLPNDYEINDALETVGQMGGQVVRIYALPIRRADDSDDVPRYITGPGEFNE
                     KAFVALDRVLVAARRHGVRLIVPFIDQWSWWGGQAELAAFRGKAAKNCWTDPELIDDY
                     KQVIAFVINRRNTLTGVCYRDDPAILAWETGNELNAPASWTQQIAAFIKSIDRNHLVI
                     DGATYDTLPKSRLEDPNVDFVQTHHYENNAPAMIDRIQRNCRAARGHRPYHVGEFGFL
                     GTQSLRAVMDTVIEQRVTGALLWSLRYRSREGGFYWHHEPAGGDLFKAYHWPGFEAGE
                     AYDERGMMRLLRAKAYEIRGLTPPAIPVPSSPCSVRADDGGMVSWRGSVGATCYDVQR
                     AEWPLGPWLTVGSGISEAQVQYQPQFIDESACPGKTYRYRVIARNHAGCSGPSQAGSP
                     VRISHRTLVDELRNDSQIFVKQGRLHFRQNEARKFKEDCHRLSADPDSAVIYHTHGCM
                     SAVRLFVYSQAEPKDIQIAFSADCKEFKPVEPDVQRTTTFGERVYGFWKADLYTAKPK
                     GNDCRFVKIVFKTDAQLGRVEVEYDAAH"
     gene            19691..20899
                     /locus_tag="P5316_05440"
     CDS             19691..20899
                     /locus_tag="P5316_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRR84435.1"
                     /translation="MTPLTDGALAASVRRELLENILPFWRSHSVDAQRGGFVAELSND
                     LRARPDAAKGLILNARLLWTFSAAYRYTQRAEDRLLARRAYDYLAHHFLDPQHGGYFW
                     ELNPDGSVRDGKKKIYGQAFCAYALSECHRTFGEPKALEQAVDVFHRIEEAGHDDRHG
                     GYFETLARDWAPCEDVRLSDKDMNEKKSMNNHLHVLEAYTSLLRARPDAFLADRLREL
                     IDLFERRILNPEKTHLQHFFAEDWTPRSDSYTFGHDIEGSWLLCEAAEALGDEGLLGR
                     VRDVALRMARAVLNEGLDADGGLCYEGRDGRIIDSNREWWPQAEAVVGFFNAWQISGE
                     NMFRDAAVRCWQFILNRIVDHAHGEWFWRVSRDGVPDVSMPKVSMWKCPYHNGRCCLE
                     ILRRLETGAT"
     gene            20956..22098
                     /locus_tag="P5316_05445"
     CDS             20956..22098
                     /locus_tag="P5316_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRR84436.1"
                     /translation="MADHDALIERPNEIAPGGNGVFDRYKYPVLTAEHTPIFWRYDLD
                     PQTNPHLMERLGINSVFNPGAIEWDGRILLVARVEGCDRKSFFAVAESPTGVDRFRFR
                     DYPVVLPETADPDINVYDMRLTKHQDGWIYGLFCTERKDPGAPRGDLSSAIAQCGIAR
                     TRDLESWQRLDDLRTASPQQRNVVLHPEFVDGKYAFYTRPQDGFIDTGSGGGIGWGLS
                     ESIEHAVIGPERIVHDRKYHTVYELKNGLGPAPIKTPDGWLHLAHGVRGTAAGLRYVL
                     YAFMTALDDPARLTHEPGGYLLAPSGEERVGDVSNVTFSCGWVAKSDGRLFIYYASSD
                     TRCHVATTTVERLVDYCLNTPPDPLRSYACVQQRIELIRRNLSLRR"
     gene            complement(22153..23373)
                     /locus_tag="P5316_05450"
     CDS             complement(22153..23373)
                     /locus_tag="P5316_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HRR84437.1"
                     /translation="MLSIPMTMMVAVLTTAETPEPTFTIRMPSEKIGYPGVEYWGAID
                     GPDGKTLWSFAIYAGKDAWTIPLRAPAPGTYRLTRVEKRAGDGQTDIELAEISPPQID
                     VAKARQTEAPSPKTLVEAGEFKRIFAAEEPWCINDHTIVQGPDNTWHLFGITHPKPLD
                     FFKDPGRRLAHATATSLLQDPWQAQPPAVTADWEKYKEYLLWAPHVIRSNSIYYMFVC
                     AGDKDTHKYRIHLLTSPDLKSWMRSPDNPMVVDGFDGRDPMVLRAENQWIMYYTATTT
                     PDGGNHTVVCVTSKDLTHWGDRKVVFIHPNAGSFGGPTESPFVVRRGRHYYLFVCDND
                     WTHVYLSRDPFHWDIEQRVGRIRSHASEVVRDAEGKWYITHAGWMSGPVSIAPLKWKD
                     DLDETPSSIPPAFK"
     gene            complement(23409..24467)
                     /locus_tag="P5316_05455"
     CDS             complement(23409..24467)
                     /locus_tag="P5316_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR84438.1"
                     /translation="MAVLLVFPLTTLCAASGSQPADGRPARLSVRGTEILRPDGNPIH
                     FRGFNLLWWVPPTAQDVVDIKDLGANCVRYQFGYVPSGRFDPRQLRFLKRHVRLFTSQ
                     GLWVIPNLHTFHAGGRPGSGNVWNTPDLQQEFLDMWNYVLDLLKDEPFIAAWEPMNEP
                     HDVDRTELAPWYREVIAHFRRKDPRTPIVVEGAQYSGPEELLDYLKLDEPNLIYSFHF
                     YYPHEYTHMLSPESEPLLEYPGKWGKAALAERMSTAVRFRDRHQVPVFCGEWGVRTGA
                     PGYVQWLKDVGSLLEEHKIPWAHWAWAMQRHWPVNDTFDLNRQKTEIYGVVSAILRNA
                     LSGDQAKLDHRNATRQAE"
     gene            complement(24576..27194)
                     /locus_tag="P5316_05460"
     CDS             complement(24576..27194)
                     /locus_tag="P5316_05460"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HRR84439.1"
                     /translation="MRARRSASTVPVGFKPVGSDVPDLMLMAAALVCLVTGAARAADV
                     LKQYYAHPTVEDGNGVIAPWYQGQNGQFDERMQIAVDIYKRYPWTKPGQAVIPAPHIV
                     YNTHWKIDNDGTISIPPTQPWMCGDLSQRAFSIIRGLSEYYRYSGDPIAFVYVPITVD
                     YILDYCLTDPDHAWPSFPIATPTKGIGYRKADPNVPNQLDLCAYVGLEVLRAHKLLGN
                     ERYLQAARHWGDVFAEKCNFSDPQMPPWSRYMSPEYMLWSDELTGSTTLITEFLDALI
                     DMGYEGKDGLIVKARDAGRAYVIDQILPRWIDNEAWGRHYWDVEGDWISGGASWICGY
                     FLDHPEAFPNWRNDVRNILSLVFNRNCVDPASRGEVYSGAWAFPESFVCCGTSLSYNQ
                     YTYAAPLIQLGELAEDEWAREVGRRMLMMATYDSRENGVVLDGLAGNVVAAADWLNLA
                     HPWPLCQILAAMAWRPETYGPSRENHVMRTKSVVTSVNYDKGVVRYSTFDAPAGNIDV
                     LRLAFEPQSVSADGKALAKRADLKENGFTAERLPDGDCIVSIRHDGAKNVSVRGDDPQ
                     EVANDDAFSYTGQWSTVRMDGAREGSLHVATDAGAALTFTFTGNQFRLIGTVDDKGGL
                     ADVYIDGQKQMAPLDCWNPKPRHQQLIYRKGGLADGKHEIRIVARGEKNPLSAGSGIY
                     VDGIQYSAATGKSNFGEGGGPTTAQRLILGYTGRNDYVDSQGRSWRPGTEFVVRLGAS
                     VDAVARTWFTDRRSLYIGNTPDPELYRYGVHAQEFCVNLTVGPGRYRVGLHFADTNTR
                     SILRVLVNDEQVIEKMKIAEAAGGIFRACNRWFGGISPKNGVIEIRFIGCDKTEACAQ
                     AIEVEPEATDSGNGGS"
     gene            27267..>27668
                     /locus_tag="P5316_05465"
     CDS             27267..>27668
                     /locus_tag="P5316_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR84440.1"
                     /translation="MNRLILAFVVLVLPPLVSVARGQVANRHYPSSRPPLRQTRFVEL
                     PLGAVKPQGWLRDQLRIQAEGLTGHLDEFWPDLARSAFRRPVTERYPFEKRPAPLTYR
                     GQLHWNPEGCTGCTLCVKDCPAEAIDFFILDK"
BASE COUNT         4997 a         8378 c         8698 g         5595 t
ORIGIN      
        1 catggtcttg cgggtcacgt cgagcggtcg ggggatgatt gcgcccgtcg tatcctgcgc
       61 cgagttgcct ggggaaacgc ctgcgggtca gcccgcggta ttcgatgtac gagccggcgg
      121 aggcgcaagc gcatcaagga gcaattcggc ggcgggttgc ggtctgatcg gcgtcgcccc
      181 cgctttcgcg tttccaggat tctacctgct gcatcgtcat cgtcgtcggg gcgccggccg
      241 cgtccgttag caaagccggt cgcgattggt gcctgtcgtc acgggttagc gcaatccgga
      301 tccgtcggta tcttcggacc ggcgaagcat tgcaggaata tcgcgaagtc gtccatgtcc
      361 acatcgtcgt catagggatc gagtttggcg ccctggcatt ttggatcgtt ctgaggaaca
      421 aaggccccgc tgaagcattc ctggaagtat ccgaagtcgg cctggtccac gtctccgtcg
      481 cgatcgaagt ccgcgacgcc gtacggtgaa cctgaagcgg tcgtggccgt ggtcgagtag
      541 aaacggctcg catcctggaa gtaggcgaac gccgagtagt aaacccgtgt accgttcacc
      601 aggcccgtgt gaaggaacga gtcggtagcg cccggcgacg cgctcctgtc gagcagcagt
      661 tgtccgtcgg caggccccgc ggggtgccct gtcgtgctgt agcgaatcac cacgccgttg
      721 cagttgacgc tggtggagtt ggtccagttc aattggttct gagcgttgcc cggtgtcacc
      781 gtcagcgagg caaccggcgt cggcggctgg gtaagaagca tgaagtcgac ggtagtcgtc
      841 tggttttcga gcacggtcac gttgctcact gtctgggtcg tgtagaaggt cttcgatgcc
      901 tgggcgctgt gtgtgccaac gggcacgagc agcgtatagg caccgttcga ctgggagacc
      961 gcactgggaa caccggcgac gatcactgtg gcgttcggca ggccagcccc gaggttgtca
     1021 cgaacgaacc cgctgattgt tcccttgggt cccggcggca gcgaagacgg cttgaccgca
     1081 taccattgtt gaggcttgtc gctaccccag tactcccaaa tacagtaaag cgcgccggtg
     1141 tcgtccgccg cgaccaccgg ggtgaccgtg aacgcgtccg tctggccgtt catgaccgat
     1201 tcctcgcccg accacgctcc cgcggtccat cgtcggccgt agacgtggaa cccacctccg
     1261 cccgtcggtc gggccacaac catgtagaag tcgttcgtcc cgggcaccca gcagacgtcg
     1321 cacgaccccg cgtatccgcc cgcgctcaca tcgatgaccg gtccgggccc gactgccggc
     1381 gtatagagct gcgacttgat cctctcgtcc tgatcccaca gcagcatgac ttgccccgtc
     1441 gcgctgatgg ccatgtgctg gcggaccggc cacgcaccgc tgttcaggtt gatctgtggt
     1501 ccccaggagc cgctgtatcg ccgcatggcc atactgctgc cgttcgggtc gtacgagcgg
     1561 tagaccgagc cgtcgagggg tgaccgggcc atgccgaagc agtcatattc ggaattactg
     1621 tgcgatccga tgtccgcttc ggccgtccat gtcgatccgt tgtagcgggc gaaatagagc
     1681 gatttatcgc agccgccggt cgagcccgag cggaagtagc tcatgatcac ttcggcgctg
     1741 gttcccgtgc cgaacgcggt gatgtgcggg tgtttggcgc agccgcccgt ggccgacagc
     1801 acctgggtga aggggaacga tgctcccccg tttgatgatt tgtaccagcg gacctcgggt
     1861 ttgtcgtttt tctcgcccca ggcccagtcc tcgaacacaa cgtggatgtc gccgttcagt
     1921 gcctcggcga tcgccccgtt agccatgaag tgtgtccccg gaaagacgtc gatcgggctg
     1981 gtcagcgtgc ctccgctgta gcggcgatag cggatccggt tgttgacatg gtagattccg
     2041 tgaaaaccgc cgctgcgagc cgccgcgatc ttggcggtct tgacgtccaa ccccgcctcg
     2101 cccggcaagt taaacaactg ggcgttggac cactgggcct cggtcgcagc agtcagaaga
     2161 gccatgcaca gtaccgagat cggtcctgcc ggtcgcgtcg catagactct tgtcatcatc
     2221 agaattcctt tgctcctcaa gggaccatgc aatacgggtc ggccgcaaca cccggcccgc
     2281 tgaaacagcc catgaacatg ttgaagtcat cgacatccac gtcgtcatcc gcatcaaaca
     2341 ggcagtctgc gcaattcagg tccggctgag gcacaaacgc gccgctgaag cattgctgga
     2401 aatggccaaa gtcttgctgg tcgacgtcgc cgtcgcgatc gaaatcggcc ggtccaaacg
     2461 ggcgccccgc agcggtggcc ccaggcgagt acacatacgt gtcggagtgt gcgaacgccg
     2521 tatagtagta ggtcacgccg ttggtaagtc cggtgtgcgg gtatgtgtcg gtggtcccgg
     2581 gcatggccgg gcggtcgatc agcagacttc cgtcatcggg cgcggcgggg tatgcggttg
     2641 tcgagaagcg gatcatcgtg cccgcgaagt tgcctgcggc aggattgacc cagctcaggg
     2701 tgttcaatgt atttcccgcg gtcgcggtga agctggtcac cggctccggc ggcgtcggaa
     2761 ccaatgagaa gtccgcgatt gctgttgagc cggctgttac gctgatgccg ctgagggtct
     2821 gactggtgtg ccagggcttc gatgcggtga cgttgtaggt cccgaccggt acggtgggaa
     2881 tgacgtatga gccgtcggcg gcagtgatgg caacgtagta gtatccggcc gccgacacag
     2941 cggctcccga cagcaaatgt ccgaaactgt ctcgaaccgt cccggtgatc tggcccgtgg
     3001 gccccggcaa cggccgaggc tgcgtgctga accaagtatt gtagacgtcc gtcccgcccg
     3061 ctccgttgct gttctcctgc caggtcacgg tgatcaggcc ggccgcatag ccgatgtcgg
     3121 cgttcaacga tgcggttcct gccgtgttgg agatgttgct ccagttgccc cacgatccgc
     3181 tttcgttgct ggtgtagaag atgtcgcggt tgttctgtgc gtcggtgaag gcgtcggccg
     3241 ccacgtgagc gatgcctgct gaatcaacgg cgatcttcgg gcggatgccg ctgttcaagc
     3301 cgccgggcag agttgccgga gcagtccagg ccgagccgtt ccagacctgg taacggatcg
     3361 cgccttcggc ctcatagacg acgtgtaccc ggttgtcacc gcttaccgca atgccgggcg
     3421 tagccggacc tgtttggagt gtggagatct cctgccgagg ctgccatacg cccccgaccc
     3481 gccgacgtgc gttcacgtgg taactgccgc tgatgcgatc cgcccacacc gcagtcacgt
     3541 cgccgttcgg tgccacggcg atgttgacgt tgaccgatcc gtacaccgcg ccgccggggg
     3601 tattggagat gttgatcgcg gggttgaatc ccgagccgtt ccagtcggca aagtatgtgt
     3661 cgtagttgtc atcggcgtag atggcctcat cccacgccac caccgggccg atgccgttgc
     3721 gggccgcgat cgccgggcgc agagatttga tcggcgtatt gctggcgttg aggatcggac
     3781 cccacacgtc ggcggtcatc ttgcgatacc agatctcgat gttggtcccg tccggatcgc
     3841 agatccaggc agtgtgcagg tccgccccgt cggcggcgac aaccggcgaa tagctctggg
     3901 ccggaccggt ctgcgagatc tccgttgggc cggcgaaggg gccggccgta ttgttggtgt
     3961 accagatgcg gaagagagag ccgccggttc gctcgcgcca gacaacgtgg caacggccgt
     4021 tcgagtcgac cgtcattcgc gggtcgaggc tgtcttcgga cgttgaaacc accgggccgg
     4081 gcgttgtcca ggcgcagagt gatcctgcgc tgcacagcag ccagaggccg gcgacggccg
     4141 ttgttgatct gatgcgtctc atttttctct tcccgtcccg ctgttcgcga tcactggttc
     4201 gtgcaattca ccgctggtgg caggccggta ccggccatgc agccgaggaa caacgacaga
     4261 tcgtccgcgt ccacgtccag gtcaccgtcg agattcatgg tctggcaggc aggctctgtc
     4321 ggcggcacaa acgcgccgct gaggcacgtc tggaacagtc cgaagtcctc ctggtcgatg
     4381 tcgccgtcac ggtcgaaatc caccgggctc aaagccattg ccgccaggtt cactccaacc
     4441 gagtactcgc ggttggcgtt gtaggtgaac gcggcatagt acatccgctg accattggtc
     4501 aggcccgtgt gagcaaagct gtcactgctg ccgatcgcgg ccgggcggtc gcatacaagc
     4561 actccatctt ccggactggc cggataaccg tctggattgg accagacggt cgtacctcga
     4621 tagctcatgc tgccggggtt tgtccatgac agggcaaccg cgccgtcgag cgactgggcg
     4681 gtgaacgacg aaaccggatc cggcgagccc aggaccgcag tcgtggaata gaagacatac
     4741 cgctgctcat taccatcgac gctcttgcgc tcgcaccaca cgacgtggag cttgccggcc
     4801 aggtccatca cgatgcccgc cgtgtaggcc ttcaacgtat tcggacccgt ggagatcatc
     4861 tccgagtacg tggccccgct gtattgatac tggcggtaat agatctccgt tgagccgctt
     4921 gcttgtgcgt agacggcgtg catggtgttg gacgcgtcga atatcgcatc ctgcacgaag
     4981 tcgatacccc agttggccgg cgcggtccag gtactgcccg tccagcgaga gtatccctgt
     5041 cgattctccg agccgccgaa gcggtcctgc cagaacacgt tcggcagatc attgtggtcg
     5101 aggaagatct tgggcgcctt gccgtagttg cccgtcgaga gctggtacca ctcgattgat
     5161 ggcagccacg ccgccgggtc gccgaggttg gtgttcttcg tgtaatacgc ctggaagccg
     5221 ttgttcagga aatcgtcgtg ccagacgaca tggacggtgt tgccatcggc gtcgaccgca
     5281 atgtccggat cgcatgagcc gtaggaagta ccggcattgt tgcgggtgat attctgaatc
     5341 ggttgccaaa cgcccgcctg acgtttgcga tacatgatat cggcctcgcc gccggtgagg
     5401 ccgtcctggc tccacacgac gtgcaaatcg ccgttgcggt cgcgggcgat acatggactc
     5461 gtgcagttgt tgccgtcgtt accttggccg ctgctgagca tcgtcgggcc ggcccaggtc
     5521 gtgccgtcga actcccagaa gtagacggtg taaggcgtgg cggtggtgtt atccacgcgg
     5581 gcgattccca cgacatacac gtgcccgtca gggctgacgc acatatccga ttcgggctcc
     5641 ttgcggtctg ggccgggcag atcctgtggc gtcgtccaag tcgtgccgtt caggaaacga
     5701 tagtagatgt gacccgcggt cgccggtggc acggtcggcg agtacacgag gtggatgcgc
     5761 cccgacgcgt ccatccccat gctcggcttg ccgccgggcc aggtaatacc cgggtggatg
     5821 gagtattccg gcgtccaagc atgcgccgcg gcgggcagaa gcaaaccaat tcccaggata
     5881 ccagagacag tcctcagcag atgcatcggc ggttgcccct cgggcatgcc gtgcgaatcg
     5941 cagcatctgc gaggtgccgc cattcgcccg gcatactgcg acctgatccg ccgttcgcgc
     6001 ggcggtcagc gataggaata ggagctctca gcttgaacgt cctaaggcgg accgcctttt
     6061 tgcgggccgc aacccaagcg caccgcggtc cagaaacatg cgcgcaaaac gcgcaccagt
     6121 cgcgacggaa aagactaaaa ggaccaagca acgctttgcc ctccggtcag cgcgacccga
     6181 agaccacgac tctactccag gatatcttcg ctaaaacatt ttagcaagcc tttgggcgtc
     6241 tttgggcggg atgtggttgc ggggggtagg tagaggatgt gatcacccgg cagggcgggg
     6301 gagacgcgag aacgggccgt cccagctcgc gttgcagctt ccaagcgggg atcaaaagcc
     6361 gtcagggcgg tatcacgccg gcatcaatga gacaggcggt agtcgccggt caaggggcga
     6421 gccgccggcg actttctact cacctgaaca cggcggtaac ctcaacaggc gtggataggt
     6481 cttcgatgcc gggcacaaga cggatatcct ggcccagcag gttaccgggg gcaatcggcc
     6541 ggtcgttgac aaaaacctcg gtcggcttga ggtcggccgg gtgacggagt cgcagccata
     6601 cttccttggg aggcgttcgc gtcgggatcc ggatcgtcgc ggtgattcgt ccgctatcgg
     6661 actctgagtg aatgaccaaa ccggccgtac cgaagatcgt cggagcgcgc tcgatccgaa
     6721 tatccttttg attctcaagc cactgtttgg gaacggctcg cccgtaccac aggcgatcgc
     6781 cgtcctccca gatcatgagc tgtcgtagcc acatgatgaa gcgcgactcg tcggaagtct
     6841 tgtaaagcgg cccgccgggc ttgcccagcg cctttgccca ttcgctgaaa cagttgacat
     6901 cgggaaagat gctcagtgca tacgtgttcc agaacgctcg cagggcggcc gggatctcgt
     6961 cgcgggccat gtagacggtt gccgagtcca ccaggcaggg ttgaagggtc acgccgccgt
     7021 gttcgaacca ggtctgctcg aaatccccaa tgttgaagcc ggattgccag gagaagtaga
     7081 tgttgtcctc caaatcgtcc agcatccagg tgatcagggg gtcgttcggg gagacgactt
     7141 cggcggtggc caggtgtatc gcggggtaca gggcctcacg tatccagcct tcggtcagat
     7201 gccgccagtg gaagacccgt gaaggcgtgt agggaatgaa gtgtccgtcg cgcaaccgca
     7261 ctgctgccgc tcgggtggtg gcctcgcgca tgttgcgctc gaggtcgttg cggtattctt
     7321 cggcctcgac ggcgatacgc tctgcctcag gatggcctgc gtccgccagc gcctgtgcga
     7381 cgcgcttcat cccgaggtgg aagtaggcgt tggtcgcgaa ccagtactgg tactcaacga
     7441 cgtcttcgag cgagcaagcc ggcgccatgc catgtatcga aggccgggga gagccttgct
     7501 tgcccatagt gctcttgcgc tcattgatca ggaaatcgca ccccttgatg agttgaggcg
     7561 cgacgcgatc aagatatcgg cgatcgcggg tcatgaggta gtggtcggcg atgccccaca
     7621 tggtgaaccc gtggttcata gcgtactcgc cgtgtgtata ggccccggca ctgtggaaca
     7681 ccccgtcctt ggtcgagaat cgccccttga ggggttcgtg tccctgaaac ctgagcatcg
     7741 gttcgatgaa tcgctccgcc tcgacgtgct cgccgcgcat atccatcgac cgggcgatca
     7801 tgaccgtctc gttggcaaag acacggtatc cgacggtgcc cacgccctgg ttatacaagc
     7861 cggtctccgg atcgcggtcg gtggtgatga tgttgtgcca gaggttggcc cggtagaagt
     7921 ccatgatggc ggtgtcgggg acatcgaggg tcatgccgcg tccgagacgg gtctgccagt
     7981 atttcatgat cgccggcgcc tcgacttcgt aactgatctc gcgaatacga gtcagctcct
     8041 gcggttcgag cagctcgaca aacggcgtct tgaagtacat gaggcgcgtc tcgccgggcc
     8101 gcagttcggc ctgccaacgc aatattggat gatcctcggg ctcgtcacct cgctggaccg
     8161 tccagcctgt aatgccgccg ccggacggct tgtcgttgga aatcacgccg cgcagagcgg
     8221 tcagcccttc gggtaccggc cggttgctcg gacgctcgat cctcatcacc ccgtcgggat
     8281 ccaagctgat cggcgacttg tcgtggactc gcaggttgag cgtcgcggtc cgcgtttggc
     8341 cgtcggtatt ggtgaccgcc aggcggccga gcaacactac ggtctcgtcc cccttgcggg
     8401 tcacatcgtc gccgtagtcg ccgagcagag tggtcgttac caacgaatgc cgaaactgca
     8461 tcggtccggt ataccactcg gcgtgaatga gcggcagatg tccttgttcg aggcggcgaa
     8521 tgcatccttc gcgatcgcgg ccgtcaaaag cgggctgctc gcgcgtgtcg aggaggtaat
     8581 cgagtcgctg ctgaagtcgt tgggtgccga aatcgctctg gaagctccaa cgtttggcgt
     8641 cgcggccgtt gtcgttgttc aggctcattg cccagatgga ccagtcgccg gccggtgaaa
     8701 cgaagaattc ctgccgggcg gacggcacgc ccagcggaac ccatggagcg ggcttgagcc
     8761 gcggtatgcc cgccatagcc atttcgcggg tcatctcggg tttctcggtc agtcgctgcc
     8821 gcaccggacg tcgccagttg ccggtgggtg gaccgggatc gttggccagc gtcaggccgc
     8881 ttgaggacga agtcaccaat gtgccgaaat ccggcagccg catcgcccca tccgcaagca
     8941 catcttgcgg cacaaaggaa aagccggtcg accaagtgtc gctgccgaat ctcaccgtca
     9001 cccgggtcag atcattggag ttcgggtcgc ggttatcggt atatcgcagg cgcacgcgta
     9061 ctcccgacga gccaccggcc tccttttgcg cagtccattt ccgtccttcg atctcggcgg
     9121 accgcggcgg tgccaggctt ttcatttcga gcaccacgcc gttgatgacg tcgagctgtc
     9181 gggtgatgat ggcgtccgtt gcgtgccggc tctcgatgtc gaactgagct tctctccacc
     9241 gcgactgccc ataggcggag agaacggcgt cggacggcag gcctctccct tcggccacga
     9301 cccgaacctt gagcgttcgc cgataggccg ggggttcctt ccatgccgga ttcggctcct
     9361 ggtcccattc ttctttgaca agtggtggca acctgaacac gagaccacgg cggggatcct
     9421 tctcgacggt cgccttgacc ctcacccagc ggccattgaa cggatcatcg agacgcatcc
     9481 acccgcccgt gccgttgccc ggccagacgc ttccccacca ctcgacgcac agggacccga
     9541 gcagcgaatc atcgactcca ctgagggcga tctcggacac ttcgcgagga ttgtcccatt
     9601 cgacaccgat gccgtggtcg tcggtgagcc tagtcgcaaa gcgagccagg tcgacgggtt
     9661 cgccgacaac ggcttgttca ggaccgaccg ttgcgcccgc agcaagaggc gaaacgagga
     9721 cgaccaggac tgcgaaccga tgaaggggcg ccatcctcac catgatgcct cccttgccgg
     9781 aatgaatctc tcagtgcata ccaaagcgtt cggggttgaa gagttcgacc tcgtcgtcgg
     9841 cccaatacgg cagcaatccg tcgatgctca gcaccttgag attacagact tcaagggccc
     9901 atccccgcgt gtctccggaa gcgacggtct tcagctccag ttcgtagcgg ccggggtcct
     9961 ggaagtcaat gaagtgccga acggtcgcga ccggccggcc agcggggttg tatagcgtcg
    10021 ccgtgtggaa accctcgccc atggcttcaa acctgatttt cacttccttg gctccctcac
    10081 gcacgaaccc gactgcgcgg gggagcatct gcggcatgtg ccggcctgca acatcgacaa
    10141 cggtcatccg ggcggcgcgg tcgcgatgca cctgccagcc gaaggcatca ccgccggtca
    10201 gacgcattcg gaaaaggccc ctgttgcccg gcaggttcag gaccgcaggc tcgaaatagt
    10261 tgccggcgtc cttgagtaat acctgccgca aagtattgcc cgcaacgtcg aacagcgtga
    10321 tcctgccctc ggccaggcct tcccgaatga ccaggatcgg cgcgggccgg tcactcacgt
    10381 gcaggaaaac ctcaacgtcg ggcgctttga accgcgtggc cttccacgac gagggctccg
    10441 gcaacaccgt tgcttcatcg aacatgcgga acagcccgta gcgggcggcg aatcgcggga
    10501 atcgccacat gtgtgtggcc tccgaagcca cgttgcgatc gtggaggaac tcgatgctgc
    10561 tttgccagca tcgctcgagg gcatccaggt atcgcggatc ttcggtttgc tgcaaggccc
    10621 acgagaacgc gttgagcgtt cggcaggcgt actcgggata gaacccgagc gtccgatcgg
    10681 cgacccggag gatgccgtcg gcgagcttgt cgtctccgtt gttctcctgc accgagagca
    10741 tgccgttcat ggcgattccg ttgaagaagg cgatgcggtt gtcccatcgg atggcaaact
    10801 tgcccgtcgc agcctgacct gcgagcaggc tgttgcagag ggattcacag gccttgaggt
    10861 atttctcgtc gcgagtgacc tcgtacatgc cggtgagcag atacagcgac cagccgatct
    10921 gccgttcgtt gccgaccacg gagccgcttt cgcgcgtgag catggcggcg acgaaatcac
    10981 cgaccttgcg ggccatttcg agttgccgcg tctcgccggt caacaagtag tgaagcagca
    11041 cgccccgtag aaagacgtgt cccaaatcgg cctcggtggc ggtgtgcaac gggctgtgtt
    11101 tccataccat gccgttgaag ttgttcacgt ccacgtccgt ctggtggcgg gccatgggct
    11161 cggcggcgtc gaggtaccag ggatcaccgg tccgcaggaa ttcgagcagg tagttgtacg
    11221 gcacgtcata ttcgaggttc tgataagcgt tcttaccttt gtacgggcca cccatgtagc
    11281 cgtcgccgaa atcgcgcatg ccgaaaggca tccggttgac gtagtcgcgc atgtccgcct
    11341 cgcgccagca ctcccaataa gggaagctct gcaaggcttc cgtgctgcgc ggcaagacgg
    11401 caccgaatgc tttcgtgcca cacgcccaca tcggcggcat ggtcatcctc aaggggtcca
    11461 ggaccacgtt gggcggggcg gaatcgtctt ttgccagagg gaagtcgaga atgatctcgt
    11521 gggtcttggc gagtcccgct tcccagttga agggccggct gccggtgtag agtctcgcgc
    11581 cgatcgcctc aggcgtgacg aacagcgcct tgggacagtt ctgccagaaa tggcgtactc
    11641 cgagcacaca ccaggtgtca ccctgcttca ccgccgtcca gccgggggct cgtgtgccgt
    11701 tcgtgtcggg cttgcccgtc tgcctgatca catagcgcag gtcatccggt tgctgcaatt
    11761 ccaccgtccc atcggcggca agcattcccc catcggtgcc aagcgcgaca agggcaggat
    11821 taccggccaa cgcgaaccgc acggacaggt cggccacatt gatggccggg tccggatcgc
    11881 cgtacggatc atcgcgattc tgcgatttct tgcgatcggc ccaatgagta atggtgagca
    11941 tgagcgtgag gcggtgcgac ccggcagcgg cttgtgcgcg aagatcgtat ttcatcgctg
    12001 tttgccggcc gtccgccgtg ccgaagaagc cttgcatgcg caggcagcca tgcagcgcgc
    12061 gggcttcttc gacctcgatc tgctgtgcgg gcatggccga cagggtggtt gcctcgccct
    12121 caagcgttac ctgcatttcc attgtcagct tgccgagaac gacatcgctc tccctcagcc
    12181 ggatctggtc gatcagccct tcgggcgacg cagcaacggt gatcttgtcg gtgcgcatcg
    12241 cggcggcttt gccggcggca ttgatttgca ccgattcgtg ttcggcgttc gagccgcccg
    12301 gacgcccgct gatatcgatc tcgaacacct gcccggcctc accggtgaaa tcgagcaggg
    12361 cccatcggat ggagtcatct tcccacttcg acagagcggt cgccatggag gcgatgttat
    12421 ttccaaccgg gccaaagact tgaatatcgg tctccttcct gaccgcgccc ttgggaagcg
    12481 gaaagccggc acggacatgc cctctctgtc ccgaacgccc gacgacggtg acaggtactc
    12541 tggcggcctg cttcattctc tcgaactcga tctcatagga gagctttgcc ggccggttcc
    12601 ggatggtttt gaggcgctcc tcggcttcct tcagcaccac ctgcggatcg taaggccgtt
    12661 tggccggatc ggcatccaac acacaacgaa atccaagcga atcgtgggcc ccgtagggaa
    12721 ccaggcgggc tcgctgggtg caggtgccgg cgttggcggt cccatagaag tagatcccgc
    12781 cgccgcctct catgactttg tactgcctgc cgaagtgcaa agaccttgcc gaggtgcccg
    12841 ggtagccgtc gtaccagtcg gccgtccact cccaggcgtt tccggacatg tccagacagc
    12901 cgtatggaga tgctcctttc ggcggtccgg ttccaccggt cgcctcacgg gaggttgagg
    12961 caattcggtc actgccgagg ttgcgtagcg tttcgtcgtc aacgttaccc caggggaatc
    13021 gccggccgtc ggtcccgcgc gccgcctttt cccattcggc ctcggtcggg aggcgtttgc
    13081 ccgcccaaac ggcgtaacgc atggcatcaa accaggtgat gttagtgatc ggcatgttgt
    13141 ctcgccccgc cggcggaatt ccattgatcc agtcgggcgg cggcgtgatg ccggtttcct
    13201 tcacgaaccg ggcatactgg gcgacggtta cctcggtcct gtcgacatag aaagcggcca
    13261 gatcaacttg gtgaggcgga gtctcatccc aaacctcggc cgagcccatg atgaacggtc
    13321 cggcgggcac cacgagcatg accgaaccgt ctttgccggt gatcgtgggg ggcagattct
    13381 tttccgctgc cggagcaatt gccgcctgca agaacaccag cgtcaaagcc gacatctgga
    13441 tctcacgagc actcatcggt ttctctccgg acggtggcag ggagcgcaac tctccagatt
    13501 ataaggtgac caccggcaac cggcatgcat tctcagaacc cgcccgatcc ggcgtgataa
    13561 gcgtcgccgt cgtcgcgggg aaggccaaac gacacgggga tatgggaatc gcggtttccg
    13621 gaggagccgg cactgacagc ggaaccgtca ttgtcgtcgt tcaggcgaga acgtacgggc
    13681 aagaccaatg acctgtccgg cgaaggtctc cgggcgaggc cgtcctcttt ctctgctctt
    13741 gccttctctc caaggcgtgt ggggcatgtt ttctttagtg tggggtccta ctcaacgcgc
    13801 gtccagacgg tgtagccgtt ctgctgcgtc tccctcaacc acggagcgat ggtccgccag
    13861 ttatcatacg gcgggtcgtt atagctttgc aggtagatgc tcgtcacctg actcggtcgc
    13921 acaaactgcg tgtggccgtc gacgaacgaa acgttgagac cgtcttttcc gtgattgttg
    13981 gccggatcgg gatagttgtt attctcgtct cctggttggc cgctgccgcc ctggtcgctg
    14041 tcgagaacca gcagacatgt atgcgggcgt ttgatcgtgc tctgcttctt gaccaaacag
    14101 ccggttctct gactgtacaa gggctcgacg gccggtttcc gaggcaaaca caactgctgg
    14161 ccgacgcttt ccgatctgcc gtcgatcttg gttcgatcga ggtagatgct gggaccgtcg
    14221 aaccagcccc agatctcata gctgtgtcca cccgtatcgt cggcggcgtt ggcggcggag
    14281 tgttccaggt cgtcaagaat gatccgatcg taaaaggtgt tgaaagaccg acgattctcg
    14341 cgaacgacgt ttcttgtgcc agggcacaaa gccgtctgga cacctttcaa gtacttcgga
    14401 aagatgtgat tcaggctgtc gcttcccgtc gaggcggtgg aggcgtagac cttcgcgggg
    14461 gtatcctcgg actggaaagt gcaggcgatg gcaatctgac gcgcattgga cgcgcattgt
    14521 attcgtcttg catgttcgcg agctctgctg agcgatggca gcagaatggc gatgagcaaa
    14581 gcaatgatgg ctacaactac aagaacctct attagcgtga aacccgtgga gaccgagcgg
    14641 accacgcgag ttggttcatt cctgtgggtc atgagcatgc tccttgcctt ggggcatctg
    14701 cgaggcgccg cagcgtcgat gccgggaccg ctccccgacg ccgccgcaac cgtaaagcac
    14761 tgcggtcacc gaggtcaaca cggccgttat caccatcgcg gccgtcgccc tctgttatca
    14821 ttatactgat cggcaaggag aagggcgggc agaatctata tcgatattgc gacttggaca
    14881 atacggattg cgacgcgcag gatatcactg tgcgtatgga atgcggggcg acctcataag
    14941 ccgcgcccgg cgtccccaca aggcggtgcc aggacttgac ggtgaaccaa ggggttctca
    15001 cgccgtcggt aagccttttt cctcacttaa ctggctgtca ggcactctga ccatgcgtcc
    15061 gttccgagtc cttcgcgttg tccagccggt cgtgctcgcc gctgaggccg cggtggccaa
    15121 tcaaggtctc attatcatcc tccggccgtc cggccggggg gggtactgcc gcagcgaccc
    15181 gtggcacgca agtgcttggc ccaaaggaag atacgtcaca cctgccgaaa gacggcaaga
    15241 gccaccgcat gcccatccgg cgtgctcctg tcgcgtgacg gccgttgtgt cgtggctccg
    15301 cacgaccgcg ttctcacgtt gatcgacgcc cggaccccag acgaatcccc gtgctgcccg
    15361 agccatggcg gcaagctacc cagccgcttc ggggagggga cgtttatagg cgtccagttc
    15421 tttatgatga actcgcctat ctcggcggag gaaacgacgg attctcatcg tcctgagcgt
    15481 ggccggcggc aaacgtactc ggaccatctc catcgcggcg actttgccgg ccgcgagcgc
    15541 gcggacagcc ttgtcggcga tatcttcaag acgatccttc tggttgtgga atccgccgct
    15601 taatcagtcc aacgtcgcgg ccagcctgct gcaggattcc ttcgcaagcg aggccgcccc
    15661 ccgatgccgc gttgagacgg tttgcgaaga ggctgccccg cacgacgtgc tggggctctt
    15721 tggatggctt gatgggcagg ccgatggtct ggcgtgccga gacaacggat ggtacgattg
    15781 ccgcggaaat ccatatgaag ctgcacgcgt tggatatcac gatcatcttc gtttacttgc
    15841 tggtcgtcag cctgatcggc gtgttgctgc gacgacgggc ggctcgaaat ctcaattcct
    15901 accttctcgg cggcaaggag ttgccctggt atttcctggg cctgtcaaac gcctccggca
    15961 tgtttgacat ttcgggcacg atgtggctgg tcacgatcat gttcgtttac ggtctcaaga
    16021 gcatctggct gccatggctc tggccgacgt tcaaccagat attcctgatg gtatacctgt
    16081 cggcctggct gcggcggtcg aacgtcacga ccggagctca atggatcggg acgcgtttcg
    16141 gccgcgacat gggctcggag ctctcgcatg cggtcgtagt cattttcgcg atcatcggct
    16201 gcctgggttt cctggcgtac ggctttgtag gcctgggcaa attcattcag atcttcattc
    16261 cctgggagat catctcgccg tatgtgccat tcaacgttcc cgcgcaatac gtgccccatg
    16321 tgtatgggat cgcctttacg ctggtggcgg ttctctacac cattctgggc ggcatgacca
    16381 gtatcgtgct ggcggacatt atccagtacg tgatcatgac cgtttccgcg ctgaccatcg
    16441 gtggcatcgc gatgttccat ctgtcttcgc aagcgttgaa cgttccgccc ggatggcaca
    16501 gcccgttttt cggctggaag ctggacctgg attggagcgg gatcatcgcc gacgtcaacg
    16561 agaagattgc cgccgaccag ttttcattct tcggcatcat catgatgctg atgcttttca
    16621 agggcatcat ggccagcgcc gccggtccgg ccccgaacta cgacatgcag aagatcctgg
    16681 ccacgcgcaa cccgcgagaa gcggccctga tgagcggctt cgtatcggtt gtgctgatgc
    16741 cgattcgcta cttcatggtg gccggtttcg cgatcctcgg cctgttgtac taccaccggc
    16801 tgaatctggt cgtaggacat cggatcgact tcgaacagat tctgccttcg gcgatcagtg
    16861 aattcgtgcc cgtgggcctg atgggatgcc tgctggccgg actgctggcg gcgtttctcg
    16921 ggaccttcgc cggaacgctc aacgccgccc aagcctacct ggtcaacgac ctttacctga
    16981 agtacatcaa gcccgacgcg gggaaccgca gggtcgttat ggtgaactac gcaaccggtc
    17041 tggttgtggt ggtggtgtcc acggtgctgg gctttttcgc ccaggatgtc aacagcctgt
    17101 tgcagtggat tgtctcggcg ctttacggcg gctatgtgtc ctccaacgtg ctcaagtggt
    17161 actggtggcg gttcaatggg catgggtact tctggggcat gattgcgggt ctggttcccg
    17221 ccctggtgtt gcctctggtg ttcaggcaga cgctggagtt gtactacttc ccgctactat
    17281 tcgtcctttc gatggccggt tgcatcatcg gaaccctggc aagccgtccc accgacgagg
    17341 aaacgctggt ctcgttctat cgtcgcgtcc ggccatgggg gttctggaaa ccgattcacg
    17401 agaaggttgt cgccgccgat ccgggctttc ggaagaacac ggatttcggg cgggatatgt
    17461 tcaacgtgat cgtggggacg atctggcaga ccgccctggt gcttctcccg atctgcgtca
    17521 ttctgctgaa gggtcgcagc gttctcatcg tggcgggcgt tatcgtgctg acgtcctgga
    17581 ttctcaagaa aagctggtat gatcgcctgg aagcatcggg agcggccgcg cccgtcgagg
    17641 cgtcgcgggg atcgggggcg aggttggcgt gagcgtagcc ggtttttcag gttgacgatg
    17701 gtcggctcga tgccacaact tggctgtacc catgtcggga gtacccccat gagctcaatc
    17761 gtcggcgttg ttctcggcaa gtcggttatg caccggcggg caagccgccg ccggtactct
    17821 tcgagggggc gagcgtcgcc taccgaaaga ggcgcggtcg gagggccggc ctggttgctt
    17881 gcgatggcgg cgggcctgtg cttggcaggc tgcacgccgc tgccggcgtc gaaagcgccg
    17941 tcgatgaacg actttgtacg gcgcgagggt gatcggttaa tggacggatc gaacgagttc
    18001 cggttcatct ccgtcaacat acccaatctg cattacgtcg aggacgacat gcgattcagc
    18061 cggagcatgc ccttccgcct gcccaatgat tatgagatca atgatgcgct cgagacggtc
    18121 gggcagatgg gcggccaggt cgttcgtatc tacgccctgc cgatacgacg agccgacgat
    18181 tccgacgatg tgccccgcta catcacgggc cccggcgagt tcaacgagaa ggcctttgtc
    18241 gcactcgatc gcgtcctggt cgccgcacgc cgccacggcg tgcgtctcat cgtgcccttc
    18301 atcgaccaat ggagctggtg gggaggccag gctgagttgg cggcgtttcg gggcaaggcc
    18361 gcaaagaact gctggaccga tccggaattg atcgacgact acaagcaggt tatcgcgttt
    18421 gtcatcaatc gcaggaatac gctcacgggg gtttgctatc gggacgaccc ggcgattctg
    18481 gcctgggaaa ccggcaacga gctcaatgcg ccggcatcct ggacgcaaca gatcgcggcg
    18541 ttcatcaaga gcatcgaccg gaatcacctg gtgatcgacg gcgccaccta cgatacgctc
    18601 ccgaagagtc gtctcgaaga tccgaacgtc gatttcgtcc agacgcatca ctatgagaat
    18661 aacgctccgg ccatgattga tcgcattcaa cgcaattgcc gggcggcgcg agggcacagg
    18721 ccctaccatg tcggcgagtt cggtttcctg ggcacgcaat cgcttcgtgc ggtgatggat
    18781 accgtcattg aacagcgtgt cacgggcgct ttgttgtgga gcctgcgata tcgcagtcgc
    18841 gaggggggat tctactggca tcatgagccg gccggcggcg atctttttaa ggcttatcac
    18901 tggccgggat tcgaggccgg cgaggcttac gacgagcgag ggatgatgcg tctgcttcga
    18961 gccaaagcat acgagatccg cgggctgact ccgccggcga tccccgtgcc gtcttcgccg
    19021 tgttcggtcc gggcggacga cggcgggatg gtgagttggc gcggctcagt cggggcgacc
    19081 tgctacgacg tgcaacgggc tgaatggccc ctcggaccat ggttgaccgt cggtagcggc
    19141 atttccgaag ctcaggtcca gtatcagccg cagttcatcg acgaatcggc ttgtccggga
    19201 aagacctacc gctaccgggt cattgccagg aatcatgccg gctgctccgg gccttcacag
    19261 gccgggagcc ccgtcaggat cagccatcgg acgctggtcg acgagctgcg taatgactca
    19321 cagatcttcg tgaagcaagg caggctccat ttccggcaaa acgaggcccg caagttcaag
    19381 gaagactgcc atcggctgtc cgccgacccg gacagcgccg taatctatca cacgcacggt
    19441 tgcatgagcg ccgtgcggtt gttcgtctac agccaagccg agcccaagga tatccagatc
    19501 gccttctccg cggactgcaa ggaattcaag cccgtcgagc cggatgtcca gcgaaccacc
    19561 actttcggcg agagggttta cggtttctgg aaggccgacc tctacaccgc caagccaaag
    19621 ggcaatgatt gtcgtttcgt gaagatcgta ttcaagactg atgcgcagtt gggcagagtc
    19681 gaggttgaat atgacgccgc tcactgacgg cgcattggcc gcgtccgtgc gacgcgagtt
    19741 gctggaaaac atcctgccgt tctggcgttc tcacagcgtg gacgcgcaac gcggcggatt
    19801 cgtcgccgag ctatccaatg atctgcgcgc ccggccggat gcggcgaagg gcctgattct
    19861 caacgcccgg ctgctgtgga cgttctcggc ggcttatcgc tacacgcaac gggcggagga
    19921 ccggctgctc gcccggcgag cctatgacta cctcgcgcat catttccttg atccgcaaca
    19981 tgggggctac ttctgggaac tcaatccgga cggaagcgtc cgcgatggca agaagaagat
    20041 ttacggtcag gcgttctgcg cgtacgcctt gagcgagtgc catcggacct tcggcgagcc
    20101 caaggctctc gaacaggcgg tcgacgtctt tcaccgtatc gaggaggccg gccacgacga
    20161 ccgacacggc ggctacttcg agacgctggc ccgcgactgg gcgccctgcg aagacgtgcg
    20221 actcagcgac aaggacatga acgagaaaaa gtcgatgaac aatcacctcc acgttctgga
    20281 ggcctacacg agcctgctgc gagcgcggcc ggacgcgttt ctcgctgacc ggctgcgcga
    20341 gctgatcgac ctgttcgagc ggcgcatcct gaaccccgaa aagacgcact tgcagcattt
    20401 ctttgccgag gactggacgc ccagatccga cagctacact ttcggtcatg acatcgaggg
    20461 cagttggctg ctatgtgagg cggccgaggc actcggtgat gaaggactgt tgggacgcgt
    20521 tcgagacgtc gccctgcgaa tggcgagggc ggtgttgaac gaagggctcg atgccgacgg
    20581 cggattgtgc tacgagggtc gagacggacg gatcatcgat tcaaacaggg aatggtggcc
    20641 gcaggcggaa gcggtggttg gtttcttcaa cgcatggcaa atcagcggcg agaacatgtt
    20701 tcgtgacgcc gcggttcgct gctggcagtt catcctgaac cggatcgtcg atcacgccca
    20761 cggcgagtgg ttctggcggg tatcacgtga cggtgtgccc gacgtttcca tgcccaaggt
    20821 gtccatgtgg aagtgcccgt accacaatgg acggtgttgt ttggaaatcc tgcgccgcct
    20881 tgagaccggc gcaacgtgac gaaaggacct gtgcccgtgt caaacgagca gttcaaccgg
    20941 cgtctgcagc gactcatggc cgaccatgat gccttgatcg aacgccccaa tgaaatcgct
    21001 cccggcggca acggcgtctt tgatcgttac aaataccccg tgctcaccgc tgagcacacg
    21061 ccgatcttct ggcgatacga tctcgacccg cagaccaacc cgcacctgat ggaacggctg
    21121 gggatcaata gtgtgttcaa tccaggggcg atcgaatggg acggccggat cctgctggtc
    21181 gcccgcgtcg aggggtgcga tcgcaagagc ttcttcgccg ttgcggagag cccgacgggc
    21241 gtggatcggt tccgcttccg ggactatccg gtcgtgctac cggagactgc tgatccggat
    21301 atcaacgtgt acgacatgcg gctgacgaag catcaggacg ggtggatcta cggtctgttc
    21361 tgcacggagc gcaaggaccc gggcgccccg cgcggcgatt tgtcctccgc aatcgcccag
    21421 tgtggcattg cccgcacacg ggatcttgag agttggcaga ggctggatga cctgcgtacc
    21481 gcatcgcccc agcagcgcaa cgtcgttctg catcctgagt ttgtggacgg caaatatgcc
    21541 ttctataccc gcccgcagga cggcttcatc gacaccggct caggcggcgg catcggttgg
    21601 gggctgtcgg agagcatcga acatgcggtc atcggccccg aacgaatcgt gcacgatcgc
    21661 aagtaccaca ccgtctacga gctcaagaac ggcctggggc cggcgccgat caagacgccg
    21721 gacggctggc tgcacctggc tcatggcgtg cgaggaacgg ctgccgggct gcgttacgtg
    21781 ctgtatgcgt tcatgaccgc acttgatgat ccggctcgcc tgacccacga gcccggcggg
    21841 tacctgctgg caccgagcgg tgaggaacgg gtgggtgacg tctcgaacgt gaccttctcg
    21901 tgcggctggg tggcgaaatc cgacgggcgg ttgttcatct actacgcctc cagcgatacc
    21961 cgctgccacg tggcaacaac gacggttgag agactggtcg actattgcct taacaccccg
    22021 cctgatccgc tccgttctta tgcgtgcgtc cagcagcgga tcgaactcat ccgacggaat
    22081 ctgtccttga ggcggtaaca gggttgccgt gcctcgatgg ggccgaacgc tcttcgccgt
    22141 gtcaggtcct ggttacttga aggcgggcgg gatactcgac ggtgtttcgt cgagatcatc
    22201 cttccatttg agcggcgcga tcgacaccgg tccgctcatc cagccggcat gcgtgatgta
    22261 ccacttcccc tcggcgtcgc ggacgacctc cgaggcgtgc gaccggatcc gtccgacgcg
    22321 ctgttcgatg tcccaatgga acgggtcgcg ggacaggtag acatgggtcc aatcgttgtc
    22381 gcatacgaac aggtaatagt gccggcctcg ccggaccaca aagggggatt cggtgggccc
    22441 gccgaaggat cccgcattcg ggtgaatgaa gacaaccttg cggtcgcccc agtgggtcag
    22501 atccttgctg gtgacgcaca cgacggtgtg gttgccccca tcaggtgtgg ttgtcgcggt
    22561 gtagtacatg atccattggt tctccgcccg caggaccatc ggatcgcgcc catcgaatcc
    22621 atccaccacc atcggattgt ccggtgaccg catccatgac ttgagatccg gcgaggtcag
    22681 cagatggata cggtacttgt gtgtgtcttt gtccccggca cagacgaaca tgtagtagat
    22741 cgagttgctt cgaatgacgt gtggggccca gagcaggtat tccttgtatt tctcccaatc
    22801 ggcagttacg gccggcggct gagcctgcca ggggtcttgc agcagcgaag tcgcggttgc
    22861 gtgagcaagc cgccgcccgg gatccttgaa gaaatccagc ggcttggggt gggtgatgcc
    22921 aaacaggtgc cacgtgttgt cgggcccttg gacgatggtg tgatcgttga tgcaccacgg
    22981 ctcctcggcg gcaaagattc tcttgaactc tccggcctcc accagggtct tgggcgaggg
    23041 ggcctcggtc tgccgggctt tcgcgacatc gatctggggc ggtgagatct ccgcaagctc
    23101 gatgtcggtt tgcccgtcac ccgcccgctt ctccacgcgg gtcagccggt aggtgccggg
    23161 cgccggcgcc cgcagcggta tggtccaggc atccttgccc gcgtagatgg cgaaggacca
    23221 cagtgtcttt ccgtccggcc cgtcgatagc gccccaatac tcgacgccgg gatagccgat
    23281 cttctctgac ggcatacgga tggtgaatgt gggttccggc gtttccgccg tggtgagaac
    23341 ggcaaccatc atcgtcatag gaatcgagag catgtcggta cctcaatcag catgtacact
    23401 tgccatttct actccgcctg gcgcgtggcg ttgcggtggt cgagttttgc ctgatcgccc
    23461 gagagcgcgt tcctcagaat cgccgacacc acgccataga tctccgtttt ctgcctgttc
    23521 agatcaaatg tgtcgttgac cggccagtgc ctctgcatgg cccacgccca gtgcgcccac
    23581 ggaatcttgt gctcttcgag gagactgccg acgtccttga gccactgcac gtagcctggc
    23641 gcaccggttc tcacgcccca ttccccgcag aagaccggca cctgatggcg atcgcgaaaa
    23701 cggaccgccg tcgacatcct ctcggccagg gccgctttgc cccacttgcc cgggtattcg
    23761 agcagcggct cgctctccgg cgacagcatg tgcgtgtact cgtgggggta gtagaaatgg
    23821 aatgagtaga tgaggttggg ctcgtcgagc ttgagatagt cgaggagttc ttcggggccc
    23881 gaatactggg ctccttcgac gacaatcggc gtccgcggat cctttcggcg aaagtgggcg
    23941 atcacctcgc ggtaccaagg ggccagttcc gtccggtcca cgtcgtgcgg ttcattcatc
    24001 ggctcccagg cggcgataaa cggctcatcc ttcagtaagt cgaggacgta gttccacatg
    24061 tccaggaact cctgctgcag gtcgggtgtg ttccacacat tgccgctgcc gggccggccg
    24121 ccggcatgga acgtatgcag attgggaatc acccagagcc cttgcgacgt gaacaagcgc
    24181 acgtgccgct tgaggaaacg gagttggcgg ggatcgaacc gccctgacgg gacatagcca
    24241 aattgatagc gcacgcagtt ggcgcccaag tccttgatat cgacgacgtc ctgtgccgtc
    24301 gggggaaccc accagagcag gttgaagccc cggaagtgaa tcgggtttcc atcaggcctc
    24361 agaatctcgg tgccgcgcac cgagaggcgc gcgggtctgc cgtcggccgg ctgcgatccg
    24421 gatgcggcac acagtgtcgt gagcgggaag acgagcagga ctgccatgag gagcgcgaca
    24481 cgacgacacg agcgcagaat atgatccgcg acaagaccac tgccggcggt ccgaagggca
    24541 cgatgacagg agtgttcgcg caacatgagg agctttcacg aaccgccgtt gccggaatcc
    24601 gtagcctcgg gctccacctc gatcgcctgg gcacaggctt ccgtcttgtc gcaaccgatg
    24661 aatcggatct cgatcacgcc gttcttggga ctgatgccgc cgaaccaacg attgcacgcc
    24721 cggaagattc cccccgcggc ctcggcgatc ttcatcttct cgatcacctg ctcatcgttg
    24781 accaacacgc gaaggatgct cctcgtgttt gtgtctgcga agtgcaggcc cacacgatac
    24841 cggcccgggc cgaccgtcag gttcacgcag aattcctggg catggacgcc gtagcgatag
    24901 agttccgggt ccggcgtgtt accgatgtac aagctccggc gatcggtgaa ccacgtgcgg
    24961 gcgacggcgt ccaccgaggc cccgagacgt acgacgaact cggttccagg ccgccaggaa
    25021 cggccctgcg aatcgacgta gtcgtttcgg cccgtgtaac cgaggatcag tctttgtgcg
    25081 gtggtagggc cgccgccctc accgaaattg ctcttgcccg tcgcggccga atactggatt
    25141 ccgtccacat agatgccgga gccggcagac agcgggttct tctcgcctct ggcaacgatt
    25201 cggatttcgt gcttcccatc ggccagtcct cccttgcggt agatgagctg ctggtgcctg
    25261 ggcttgggat tccagcagtc gagcggggcc atctgcttct gcccgtcgat gtacacatcc
    25321 gccagtccgc ccttgtcgtc gactgtcccg atcagacgga actggtttcc cgtgaatgtg
    25381 aacgtcagcg ccgcgcccgc gtcagttgcc acgtgcagcg agccctcccg ggctccgtcc
    25441 atccgaaccg tgctccactg cccggtgtat gagaaggcat cgtcgttggc gacttcctgc
    25501 gggtcatcac cgcggacact gacattcttg gctccgtcgt gacggatgct cacgatgcag
    25561 tcgccgtcgg gcagtcgttc agccgtaaag ccgttttcct tcaggtcggc gcgttttgcc
    25621 aatgccttac cgtccgctga taccgactgc ggctcgaaag cgagccgcaa gacatcgatg
    25681 ttgcccgccg gggcatcgaa cgtcgagtag cggaccacac ccttatcgta gttgacagac
    25741 gtgaccacgg actttgttcg catcacgtgg ttttcgcggc tggggccgta ggtttcggga
    25801 cgccaggcca tggccgcaag aatctggcac aggggccagg gatgggccag attgagccag
    25861 tcggccgctg cgaccacgtt gcccgccagt ccgtccagga cgacgccgtt ctcgcggctg
    25921 tcgtaagtcg ccatcatgag catccgtcgc ccgacctcgc gtgcccactc gtcttcggcc
    25981 agctctccga gctgtatcaa aggtgccgcg tacgtgtatt ggttatagga caacgaagtt
    26041 ccgcaacaga caaacgactc gggaaacgcc cacgcgccgc tgtagacttc gccgcgggag
    26101 gccgggtcga cacaattgcg attgaagaca agcgacagga tgttgcggac gtcgttccgc
    26161 caatttggaa aggcctccgg atgatccagg aagtacccgc aaatccagga agcgccgccc
    26221 gagatccagt cgccctcgac gtcccagtag tgccgccccc acgcctcgtt gtcgatccag
    26281 cggggcagaa tctgatcgat cacataggca cgcccggcat ctcgtgcctt gacgatcaag
    26341 ccgtccttgc cttcgtagcc catgtcgatc agggcatcaa gaaactcggt gatcaacgtc
    26401 gtgctgccgg taagctcatc ggaccagagc atatattcag ggctcatata gcggctccac
    26461 ggcggcatct gcggatcgga gaagttgcat ttttcggcga agacgtcgcc ccagtgcctg
    26521 gcggcttgca ggtagcgttc gttgcccaga agcttgtgcg cccgcaatac ttcaaggcca
    26581 acgtacgcgc agagatccaa ctgattcggt acattgggat cggctttccg gtatccgatg
    26641 cccttggtgg gggtggcgat tggaaaactc ggccaggcat ggtccgggtc ggtgaggcaa
    26701 tagtcgagaa tgtagtccac ggtaattgga acatatacga aggcgattgg gtcaccgctg
    26761 taccggtagt actccgagag gccgcgaatg atgctgaacg ccctctggct caggtcgccg
    26821 cacatccacg gttgcgtcgg cggaatcgat atcgtgccgt cgttgtcgat cttccagtgc
    26881 gtgttgtaga caatatgcgg agcgggtatc acggcctgac ccggcttggt ccaggggtat
    26941 cgcttgtaga tatcgacggc gatctgcatt cgttcgtcga attggccgtt ctgtccctga
    27001 taccagggag cgatgacgcc gttgccatcc tcgaccgtgg ggtgagcgta gtactgtttg
    27061 agaacgtcgg cggcccgggc cgcccctgtg accaggcaga ccagtgccgc ggccatcagc
    27121 atgaggtccg gaacgtctga gccaaccggc ttgaacccga cgggcaccgt ggaagccgaa
    27181 cgccttgctc gcatgtgtga tcctccgcag aatgtgacca atatggtaca gtgcactacc
    27241 gcaaagccgc cacaaggaac ggattgatga atcgcctgat actcgccttc gttgttctgg
    27301 tgttgccgcc cctggtctct gtggcgaggg ggcaggttgc aaacagacat tacccatcaa
    27361 gccgccctcc gctcaggcag actcggtttg ttgaactgcc cttgggggcc gtgaaaccgc
    27421 agggctggtt gcgcgatcag cttcgtatcc aggcagaagg actcacgggg cacctggacg
    27481 aattctggcc cgatctggcc cggtcggcct ttcgccggcc ggtcaccgaa cgctatccct
    27541 ttgagaagcg gcccgcgccg ctgacctatc gcggccaact gcactggaac ccggaaggct
    27601 gcaccggctg cacgctgtgc gtcaaggact gcccggccga ggccatcgac ttcttcattc
    27661 tcgacaag
//
LOCUS       DAOTPW010000040        27483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1824_length_27483_cov_9.172889, whole genome shotgun sequence.
ACCESSION   DAOTPW010000040 DAOTPW010000000
VERSION     DAOTPW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27483
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1824_length_27483_cov_9.172889"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="P5316_05470"
     CDS             complement(<1..1350)
                     /locus_tag="P5316_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRR84441.1"
                     /translation="MDPRTQINNTLTAGQRFLDKAFDLRGLARWLALGVVVGLVAGAS
                     AILFHVALDGLRTFSFNALIGLSPGRPGGEEAVFDVPVAGHVRYVLIVALPAAGAFLA
                     GLLVRFFAPEAKGHGTDAAINSFHRKGGHIRKRVPVIKFFATVLTMGSGGSGGAEGPI
                     AQIGAGFGSFIADRLKLGPREKRLLMISGVGAGVGAIFQVPLAGAIFAAEVLYSDPDI
                     ETDAIIPACVCSIVAYCVYGAWHGFGHMFPVSGMSFQNPLELLPYLLLALLVTAGALL
                     YIWVFYGTEGAFNRLRLPFIVKPLIGGLMTGATALALLLIVGDEHVIDVMSTGYGILQ
                     DALSGQIVEIGVLILLAVAIGKIFTTSFSIASGGSAGVFGPSLVIGGTLGGAAGLVFQ
                     GWWPGLVQNPSSFAMVGMAGFFAAAANTPLSAVIMVSEMTTGRRHAHPRHQRHALREL
                     "
     gene            complement(1463..2401)
                     /gene="ablB"
                     /locus_tag="P5316_05475"
     CDS             complement(1463..2401)
                     /gene="ablB"
                     /locus_tag="P5316_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative beta-lysine N-acetyltransferase"
                     /protein_id="HRR84442.1"
                     /translation="MNQSATMKRIATRTADEVREHRQTKFDRTARIGKAQIQYGPVSD
                     RVYVMRWNGADPEHVLPRLQSLAESENLGKIIAKIPNSVAPGFLDAGYRLEAGIPGYY
                     GGREAAVFMGRYPDRSRRHPENREKLEHVLILAAQNASESEGDVADFKSPDLELREAT
                     RDDIGQMATIYRKVFETYPFPIHEPAYLLDTMNQDVRYFAAVAGDRLVALSSAEMDPK
                     RLGVEMTDFATLPEWRGRGLASLLLDKMEIAMRQSGMRTAYTIARAPSAGINIVFARK
                     GYDFGGTLVNNTNIAGTIESMNVWHKNLAESGVKSY"
     gene            complement(2419..2913)
                     /locus_tag="P5316_05480"
     CDS             complement(2419..2913)
                     /locus_tag="P5316_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRR84443.1"
                     /translation="MADRETEILRSLRKIVRAIDMQSHRINVSFNVTVPQLLCLYAIQ
                     RNPSLTLSGLAEEISLSPSTVNGIADRLEEKGLIHRVRSSQDRRKVIPMLLEAGGRIV
                     DEAPPMLQETFVQRFRRLPEDEQNAIAAALRKTVRLLDAEAIDASPNLLPHDPCCSSD
                     VRTG"
     gene            3093..3341
                     /locus_tag="P5316_05485"
     CDS             3093..3341
                     /locus_tag="P5316_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84444.1"
                     /translation="MSDTLYVICNLTPAGGQYSTVVTAIGANGQKKWTATLPDGHTAS
                     LPVIALRSLSETMADLYQVLKRFYGGVPFDYGQCPRNY"
     gene            complement(3379..3588)
                     /locus_tag="P5316_05490"
     CDS             complement(3379..3588)
                     /locus_tag="P5316_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84445.1"
                     /translation="MANHSVVSQWESRQDSPAGMDYNTEVLLRLFMATRAGMTDKLAE
                     LIGGSLRNLKASSDHPLEIRMPHAA"
     gene            3577..4569
                     /locus_tag="P5316_05495"
     CDS             3577..4569
                     /locus_tag="P5316_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84446.1"
                     /translation="MVCHVQRRRKIHLAHPQIRSDEADLRTGQPGGLESQKAKNLVLK
                     LPHVDVRNLLTGSTSDSTSATDESTSSWRSLGEFLGQVFGGIVTVITLALGGLIKIIT
                     AVIWVVNAVKDAFVIAGTWIGETAAKIYLWFTETLPNAISSAIDTVVGFFRAIGQFFV
                     GIGRWFKNLFRSIADGIMSFLQPVVDFFRGVGRAIKAVFDGIRDLVIRILRRIPDALL
                     PASLERLKRTPLSTEVRTEDEFAAVGRTESTAARSEAATSSMPSSAEATGRMNEFAQL
                     EANMMAFANSQARDRGQAPPFQINVQVDGETIARATHNAGSDAAARSFSPVPAY"
     gene            4577..5086
                     /locus_tag="P5316_05500"
     CDS             4577..5086
                     /locus_tag="P5316_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRR84447.1"
                     /translation="MGIELAMVAPPRCVLVNVTTGESMECLLNPTQLSEKLQVNWNRL
                     AVPGLSHQVLQFQSTGNRQLSGVEFYLDRFFAVEQPGEPDIMEFRRFLRALTVPPEGT
                     EDVASTAPPRVLVIWPEVITVEAVVTDVEFQYRQVGVDGTVLVYAATVGFEEILDARV
                     TSEALREEV"
     gene            5089..5430
                     /locus_tag="P5316_05505"
     CDS             5089..5430
                     /locus_tag="P5316_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRR84448.1"
                     /translation="MPPRVGSRHSFTFGVHDADGVLHLTEREPYRYKAHTDNRVHVVV
                     QGDTLWDLAGRYFAPLPRACGFWWAIADFQPDPIVDPTLGLEAGRRLFIPSLRVLTDV
                     ILGEAGRRSRG"
     gene            5427..6698
                     /locus_tag="P5316_05510"
     CDS             5427..6698
                     /locus_tag="P5316_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HRR84449.1"
                     /translation="MNPIIDRSAPGVRITLLADERAESGEPLELGGRIIGFTFDDAES
                     KADKLSLQLDNQDLSLFDREDLMGGATLEVSWGYAGNMAVPRRVVVKKLKGFTTLEVE
                     GQALSTLMNREAKTRSWQNVTRSAVVRQIAEEHGYEGQFIDAEDTGEVLDVINQTAET
                     DARFLRRLASCEEFEFYVDDGGFHFHERRQEAAPTHVFTWYSDPGRGDILSINVESDL
                     IKRAGRVTVRGRDPMSRSTIESSSSNDTAGRSTLAEVVEVVDPETGSTSLETRNATAS
                     VHPTSAPTAGRAARESAARFRRAERATVKLSMQVVGDPTLHAKSIVEVRGVSGLLSGK
                     YYVTGVKHVVSSSGYTCELKLTRDGQGRRARQVAREQRGERNTSRPRTDGEMTQVEAV
                     DPETGSTRIEYRRDGRRIGYDDPEAGMSVME"
     gene            6701..7285
                     /locus_tag="P5316_05515"
     CDS             6701..7285
                     /locus_tag="P5316_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HRR84450.1"
                     /translation="MSYSQFDDDIGAHDTRLLGMYVGYVTHRNDPEKLGRVRVCIPGV
                     LEPFSAWAWPLGTSGGGFKDRGFFAVPEEGAEVAVFFNQGDVDAPYYLSAHWGKPDGE
                     SEVPEEAQKDPPDNRVIATPTFRIELDESDKGKKLKLTNRKTGDHLLFDAEENTITLE
                     GTTAITIRAVGAISLEAAQVTIAGRAVRPVADPI"
     gene            7297..7971
                     /locus_tag="P5316_05520"
     CDS             7297..7971
                     /locus_tag="P5316_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84451.1"
                     /translation="MALPICIEIPEIPDPFNLTLPGGIQIEHINLMEVIQPALTPLVP
                     IFDIIDTVVAVFNCIKAIPDTLGPPPDPTAIAACLPDLAEKVAKLLKLIPQLSIPLTI
                     IGLIDLVIDTLLRARSEIMHLQQQMQQILGVIDRATNLDDAGLMAIAQCAQANVAQEA
                     ANVGKSLAALGKLIGIINLFMGMVGGPEIPDLSNLAGRPLDDVVPPLNAIVDALKTAR
                     DAVPVP"
     gene            7982..8392
                     /locus_tag="P5316_05525"
     CDS             7982..8392
                     /locus_tag="P5316_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84452.1"
                     /translation="MNDHESQSRGLLVPFRRDKKRDFASDTGADLLGSKVLQALMTCG
                     ATPRSSGELPWRTAFGAGLDLLRHQRNDAALAELARVQVRDALKRWVSEAELVEVSVS
                     REDASLLLRVRFRPARRAGRGDEQPGEVTLAIEA"
     gene            8445..9956
                     /locus_tag="P5316_05530"
     CDS             8445..9956
                     /locus_tag="P5316_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HRR84453.1"
                     /translation="MAVLPTSLDYTDKDFDALRARLIALVKSVFPDWSDFSVASFGNL
                     LIEMYAFVGDVLTFYLDNQARESRLSTATQRKNVIALARMLGYKLHGAQAATAEVLLK
                     LARPPSADVVIPAGTVVRTQEVTEPVRFQLLADVTIPAGADPPEVLCVVESSETRTQL
                     FDARGLVDLDILLDHAPYLDGSAVVSAANGAYVEEDSLLGSEPNDRHFMVLVDQNDRA
                     TIRFGNGTSGAPPTGTVQIAYKTGGGAGGNVDAGRLVVIEGAFADVHGHPVQVSVTNP
                     SPATGGADRQGVASAKLLAPESLRTLTRTVTREDFEVNARRVPGVARALMLTSNEDTS
                     IDENAGILYVIPEGGGLPTVALRNLVLAQVTDVYPHTLTFQVAVQSPVYRNVDIEARI
                     YLRQGHAKAAVRDRVKANLQAMFRVSEPDGTPNPLVDFGFNIKDADGNPVGEIAWSDV
                     FNVIRDTTGVRKVGDRHGDLKLNNLPADVRLGIKEFPLLGNVTLIDGDTGSYL"
     gene            9956..10504
                     /locus_tag="P5316_05535"
     CDS             9956..10504
                     /locus_tag="P5316_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84454.1"
                     /translation="MAVANPSYEDAGAAPGQAEHWTLRTSASLERIAGFGPDPYRAWE
                     DFERWFDLVLSLGPGDVAIAFFDPLAEGYEDFEEAWANDLFMTELPSGQVVTAPFGGG
                     AVEDLEDGWDNVSYAWAWEEVAAVTGVFDGEPREDFEDQWRSNQSYAWSWGSVTSQAA
                     MFDGGTQNREDFENDWSAASTI"
     gene            10516..11211
                     /locus_tag="P5316_05540"
     CDS             10516..11211
                     /locus_tag="P5316_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84455.1"
                     /translation="MAETDWTYLNDGLDIATVDRGVTAGIARPPGGGSFLFAFNSLAA
                     VQGAVALFANLVDFAPMAKGGSIRGCVQRGPGGGPTGFSPFLYLCCQGNSVNDTAYLL
                     GLSDDDPHRIVLRKGMVGAGIPDSEGANVLLASGESFAQATWLHLRLDAIVNLNGDVE
                     LKVFKNDLVAHPLGTAPDWQPVSGMADFIDDHLGINSGSQPLTSGRGGFGVTVKDVTR
                     RCLFDHLELLRQV"
     gene            11211..11606
                     /locus_tag="P5316_05545"
     CDS             11211..11606
                     /locus_tag="P5316_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84456.1"
                     /translation="MELDAFTSRLGLGQGRIAPVNAAPASGDHLFVLGEDELGRFFDL
                     APGDHAEVVQDTDLTDVALVRANLRLRVPTSLPTTHGWEASIVVDGVKVARATCRAGR
                     ERLLTDLAANVSKLTGLHEVGVRLELVEV"
     gene            11607..12944
                     /locus_tag="P5316_05550"
     CDS             11607..12944
                     /locus_tag="P5316_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRR84457.1"
                     /translation="MPLFELPALYADNVAAIVTVDRIVLVNRDPGPAETGVPLDWTVA
                     LEVLDTGPDGVDRSATRVWVDSVLAFDGGAAPEIQPGFDGLRAEVVETADTLRIVIDP
                     AAPCASEAEVEVRVVSTTVGGGHALDESYAFFAEDRTAPRVLAAQATSPTTVQIGFDE
                     DVVVTNPEGFVFAPRAFPAVPVLAVGASAQGSVVTVELDTEMTPDVFHEVVVSGVADL
                     RGNLVLEAHDRAFFNGFRPARPASRRFDLWSMLPRHNRRGDATGDLRRFIACLQEALD
                     LVLAEADRFADVFDIERAPESFLDLILQDLGNPFPFDLDVLGKRRLASVLVEMYRQKG
                     TEAGIENAIRFFLGIDVTAITPFTGTTLVLGESELGVDWELGPSDRFARYAFDVEVDV
                     PLTTTERRQIRAIVDYLKPAHTHFVDLIEPGAPVAYDHWELGISELGETTDLH"
     gene            13017..14918
                     /locus_tag="P5316_05555"
     CDS             13017..14918
                     /locus_tag="P5316_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84458.1"
                     /translation="MAGRTDYFFRQKVTEAELDLAFELLELADRNLAADIGVYGIISG
                     AEPTPHSPVPNLTVDLTAPARAYDNLGQRIFFGTGQVVDCSVDLTGIPTEVPVAGQER
                     WLGLFLRFDRLLSDPRTDGNSQQVFFRRDESFEVVVRQGPLGPVGGASKVPVMPDELL
                     ICDVLRKNAQTQILAPDIDVTRRQAFIFAQGDAVEIVSSVWSVLAPAVDTVQAALDEI
                     DSELAGHFGGSARRHPASDIDYTPHGFVAAGNVQAALDEVVDDLSASAAGNPGAQRVG
                     ADAVSGTPHALTASNVDSHLSQLLGWLNAHLAAASGAHNASAIAAAVHSYIAGTTVQA
                     QLQEIVSDLALQTAGSAGASRIGADAVSGSPNALAAGTVDSQLAELLADGNNHVGATS
                     GAHAASAISVADSGGNLTASNVETALAEILDAFEDDHYRGNESNAGQHRAIRQPNFGS
                     GKVLLWDAVGVGSNASHFRVHADSDSIWFTVNAFWNGSAWQRDLAGNYAGGFRFSRTA
                     FEMFHDNSFTLTFTDWTRTWRLPMSGTVNSAFEMTGSTTEVGRLGYEMHNTYNATRNM
                     AVGGANTFRSRFPSTPSSITFSETSSYNTDGPPGLWDADRDGFGFFDYVNVAANSTLY
                     WFGTYTAVA"
     gene            14928..15344
                     /locus_tag="P5316_05560"
     CDS             14928..15344
                     /locus_tag="P5316_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84459.1"
                     /translation="MPIREIRADDFDQECSKCGSIVAVPLTAIAAGISRSGQMNPNVV
                     AMPVCATCGAAEFLLRSPGEEEHPSQGSYGHKHKLLVDKLHAKLVQQGRVAEGLTPEQ
                     VPTKEPPAAVMDKWFKDGLKLPPPPFQDESGRSEPK"
     gene            15355..16098
                     /locus_tag="P5316_05565"
     CDS             15355..16098
                     /locus_tag="P5316_05565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRR84460.1"
                     /translation="MNTRTFIVGGKEIEPPSGLDVLNFTDASVHRFRGRDRTGKPVTE
                     LVIHETVTRSAADTVVVLKKRRLGVHLILDEHGRFTQHGDLADDRLAHASQHNGPSIG
                     IEVVTPYYPAYLKPGMVWSTVIDAPWAHKKKYVLPTPVQAEAVARFVEWVTSPASSLS
                     IPRTWIGLHGTALAMGQIESAAKLKPGLYAHHYFGHADGAWLVLYAWLRLEAGLAPEV
                     AFDEAARRATGVRRRVDLSDLMTAAAPKE"
     gene            16102..16338
                     /locus_tag="P5316_05570"
     CDS             16102..16338
                     /locus_tag="P5316_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84461.1"
                     /translation="MDPSSFTTIAEVLKATGPYGLVAVLGWAFWRVNEKKDRELKDLH
                     ATVVKMAEAQTAAITKVESALVALKEAIREMRAG"
     gene            16560..16880
                     /locus_tag="P5316_05575"
     CDS             16560..16880
                     /locus_tag="P5316_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84462.1"
                     /translation="MAKILGSTTTYHGTEHPYLKGHKVRIVAVLKNAARPDIDVDGPD
                     YDHISDEDDLARAGGVTADDRIEVQPWIQKEGRFSFVTSDPRAVDLAYFAGAKKAAKK
                     GGQR"
     gene            16877..17152
                     /locus_tag="P5316_05580"
     CDS             16877..17152
                     /locus_tag="P5316_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84463.1"
                     /translation="MTTSSKRYPVQVEGEIRYVTVPGSGDPQPGPESEDELESFLAEA
                     IEAYAEASEVSRPRISTFADAGVLTANRGLVVRIGTAEFQVTLVRSR"
     gene            17234..17842
                     /locus_tag="P5316_05585"
     CDS             17234..17842
                     /locus_tag="P5316_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRR84464.1"
                     /translation="MNKEIRKQVKALEKLRLPELRARYAEVLGEETRAPNRTYLIRRI
                     TEALEARAAEAPEPAEAEPQATPVETAPTETAAASPETGDATETKLSKLPIAELQARY
                     LEVVQRPTASVHRRYLIWKIREAQKGRIPVGPQGSRRADGEALDFKVLPLRMEADLVA
                     KLDEARERLGLKSRMELLRRSIHLFLAREGEAEVAALFAPEA"
     gene            17843..18049
                     /locus_tag="P5316_05590"
     CDS             17843..18049
                     /locus_tag="P5316_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84465.1"
                     /translation="MAGDYDKRRLVEWLRAEIQRQTGRRYDRLDLDSLDPVSLRELQR
                     LLRDLDAEQRMAIQRARICPWRTP"
     gene            18235..19125
                     /locus_tag="P5316_05595"
     CDS             18235..19125
                     /locus_tag="P5316_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HRR84466.1"
                     /translation="MRTLRFGIEIETVGQTRERVAQAIQRVVGGTTSHVGAPFSYDPW
                     HVTDARGRTWKVVADSSLNASRDRQAEVVSPVLTYEDLPELQEIVRALRAAGAKADSS
                     CGIHVHVEAARFGAKGLRNLVKIVNKQERLIEHALGISEARRARWCRGIDQAFLDKIE
                     KQRPRDLEDINRAWYGYHNTAPIHCDASRYHGVNLHNVWFRGTVEYRWFDGTLHAGKI
                     KAYIQFVLALSAKAMRARASSSKRREFNPDTARYDFRVFLLHLDLIGDEFKTARLHLM
                     ANLNGSAAWKHGRPEQQPAL"
     gene            19140..19583
                     /locus_tag="P5316_05600"
     CDS             19140..19583
                     /locus_tag="P5316_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRR84467.1"
                     /translation="MKQTLYFAYGSNLDTARMRRRCPTAAPVGPATLWGWRLAFGGHS
                     RAWGGPVATVVRAADERVDGLLYELALGELADLDRVEGHPLSYRRKRLRVVDGRGRER
                     RAHVYILPVAWEAPPAPDYLGVLWHAYRRLGFDRDSLLEAAGGAG"
     gene            19580..19969
                     /locus_tag="P5316_05605"
     CDS             19580..19969
                     /locus_tag="P5316_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRR84468.1"
                     /translation="MSTGTNGKTRVFVYGTLLSGEPNHRLLASAARVGEARTEPAFDL
                     VSLGAFPAMVAGGRTAVAGEVYEVDAGTLADLDRLEGHPRFYQRRRIRLEDGGEVLAY
                     VFTSEQARGRPAIASGNWRHERKERWS"
     gene            19966..20211
                     /locus_tag="P5316_05610"
     CDS             19966..20211
                     /locus_tag="P5316_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84469.1"
                     /translation="MRIRMLRDGRVFQGTPKQIVEAMQYIAFGQENRTLGEYIDWLVD
                     QVQRLESIDLKVEGDTDEDKAASLVQAMMGSGLAEKM"
     gene            20455..21441
                     /locus_tag="P5316_05615"
     CDS             20455..21441
                     /locus_tag="P5316_05615"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HRR84470.1"
                     /translation="MGLVVYRSPGRQRPELRRRRASSCGRLRFPTLVVCSRLAIRKRI
                     VEAARAQVARSVNTAMVHAYWLIGREIVEVEQEGKERADYGEQVVRRLAERLGKRFGK
                     GFSYPSVKRMKQFYLSFPQGSAIPEGLGGYQKGSATLSLSPGSDVIEKGSTLLSQSAR
                     HVSTLFPPLLSWSHYLPTEEQLREDEAEHRMAVQRLGSAPVDLEGVEPCIVSVVGRLP
                     LRQESLDQSNSYPQHSGGIDNIATVLAELAEQLDPAALASLAPLSPVPWAQRLGHLLE
                     RVGAADKASPLAEHVASVVNETTALVASSPAEGAPYDKRWRLLANVQVEPDL"
     gene            21438..21608
                     /locus_tag="P5316_05620"
     CDS             21438..21608
                     /locus_tag="P5316_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84471.1"
                     /translation="MIPLDFITEWRRTAPWIHDSQVEQDLLVTFKTDNPGWGASLCAG
                     YALGFALATVWR"
     gene            complement(21653..>22108)
                     /locus_tag="P5316_05625"
     CDS             complement(21653..>22108)
                     /locus_tag="P5316_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84472.1"
                     /translation="CGECKNESKQCIDDENEIGKPRTCIAGVWKDDANQCDDVSCDVV
                     TNECGECKNHSNNICKNDEDTQAGHLETCVAGKIVLGDKCQNNNGSLKYSCEGESEMT
                     VCGTCLNDEKVCEKRGGDDRFWVYLCTHGEWKKQYTCPDGCNEQTNECN"
     assembly_gap    22109..22208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22209..23065)
                     /locus_tag="P5316_05630"
     CDS             complement(<22209..23065)
                     /locus_tag="P5316_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84473.1"
                     /translation="MMKRAFGIAAFFMLSIFLTGGCGSSEPCPIDGLDEETAAKLKCE
                     DSGGTFANDLCVCGQTFCPAGVVCKDGACAKTAFELSCEASGGTYKDERCECKNVKCD
                     AGLVCNYLTKECPESKPTGFCNAADVFCANSRMYTCGEDNTWKEGETCDKGCHSDQKK
                     CANCTSDGCEDGRLFTCNDGEIIAGDACKSGKCTDDGLKCELSCTEGDVSCFDGKLQT
                     CTSETWTTTEVCDAGCAEDKESCATRCDDGDTQCVDGKLKTCSGGVYGGEENCDNGAS
                     CKSETECGE"
     gene            23430..24290
                     /gene="htpX"
                     /locus_tag="P5316_05635"
     CDS             23430..24290
                     /gene="htpX"
                     /locus_tag="P5316_05635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRR84474.1"
                     /translation="MKRIILFLLTNLAIMFVLSIVLGLLSTQLGLDPTGIGAVVLFAL
                     VFGMGGALISLAMSKFIAKRSTGAQVITNPRSDTEHWLINTVRELASTANIGMPEVAV
                     YRGAPNAFATGATRNNSLVAVSDQLMQNMTRAEVRAVLAHEVGHIASGDMVTMTLLQG
                     VMNTLVLLLARIIGVTVDKAVFGTRNGRGIGYFITYIVMQLVLGILASIVVMGFSRRR
                     EFAADATAAKLLGSPRDMIAALQRLGSLEPGALPQSMKAFGITGAPKWAALFASHPPL
                     EKRIERLERA"
     gene            complement(27212..>27483)
                     /locus_tag="P5316_05640"
     CDS             complement(27212..>27483)
                     /locus_tag="P5316_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84475.1"
                     /translation="YALDYDQIERYCSGEGLKNHGYCANHWVILGKRSLSEFCIMPVG
                     DFGLDITLGYASLNDGASNFGKMVVCCYFLDHFKRRFHAVLRGES"
BASE COUNT         5012 a         9151 c         8317 g         4903 t
ORIGIN      
        1 aagctcgcga agcgcgtggc gctggtgccg tggatgagcg tgccgccgac cggtagtcat
       61 ttcgctgacc atgatgaccg ccgacagcgg cgtgttggcc gcagccgcaa aaaatccggc
      121 catcccgacc atggcgaagc ttgaaggatt ctgaaccaga ccgggccacc atccctggaa
      181 caccagcccc gccgcgccgc ccagtgttcc gccgattacc agcgacgggc cgaacacgcc
      241 cgcgcttccg ccggacgcta tggaaaatga cgtggtgaat atcttgccga tggcgacggc
      301 cagcaggatc aggacgccga tttcaacgat ctgtccggac agcgcgtcct gcagtattcc
      361 gtaaccggtg gacatcacgt cgattacgtg ttcgtccccg acgatcaaca gcaaagccaa
      421 tgctgttgct ccggtcatga ggcccccgat caatggcttg acgatgaacg gcaggcgcag
      481 gcggttaaac gcgccctcgg tgccgtagaa cacccagatg taaagcaggg cgcccgccgt
      541 gaccagcagc gccagaagca ggtaaggcaa tagttccagc gggttctgga acgacatgcc
      601 agacaccgga aacatgtgcc cgaagccgtg ccaggcgccg taaacgcagt acgcgacgat
      661 gctgcacacg cacgccggta tgatggcgtc cgtttcgatg tcggggtcgg aatacagaac
      721 ttcggccgcg aagattgccc cggccagcgg cacctggaat attgcgccaa ccccggcgcc
      781 gacgccggag atcatcagca gtcgtttttc acgtggaccc agctttaaac gatcggcgat
      841 gaagctgccg aacccggcgc cgatctgcgc gatcgggcct tcggcgccgc ccgatccacc
      901 cgaacccatc gtcagcactg tcgcgaagaa tttgatgact ggcacgcgtt tcctgatgtg
      961 tccgcccttt ctgtggaaac tgttgatggc ggcgtctgtc ccgtggcctt ttgcctcggg
     1021 cgcgaagaac ctgaccagca gtccggccag gaacgcgccg gccgccggca gggccacgat
     1081 caggacgtaa cggacatggc ctgcgacggg aacgtcaaaa acggcttctt cacccccggg
     1141 gcggcctggc gataaaccta tcagggcatt gaagctgaat gtacgcagtc cgtccagcgc
     1201 aacgtgaaac aggatggcgg acgcgccggc gaccagcccg accaccacgc ccagcgccag
     1261 ccatcgcgcc agcccgcgca ggtcgaacgc cttgtccagg aagcgctgac ccgcggtcag
     1321 cgtgttgttg atctgtgttc tgggatccat gtgaagccgc atgattctcg cgccatatta
     1381 tccctgttcc ccgtttgatc aaccctgcaa gcggcagtcg gccgtcgagc cacaccggaa
     1441 cgtggcgtaa ccgctgccag tttcagtatg acttcacgcc ggactcggcc aagtttttgt
     1501 gccagacgtt cattgattcg atggtgccgg cgatgtttgt gttgttgacc agggttccgc
     1561 caaaatcgta ccccttgcgg gcgaatacga tgttgatgcc agcgcttggc gccctggcga
     1621 tcgtgtaagc ggtccgcatg cctgactggc gcatggcgat ttccattttg tccagtagca
     1681 gggaggccag cccgcgcccg cgccattccg gtagtgtggc gaaatcggtc atttcgacgc
     1741 cgagccgctt cgggtccatt tcggcggatg aaagggcgac gagcctgtcc cccgcgaccg
     1801 ccgcaaagta gcggacgtcc tgattcatcg tatccaggag gtatgccggc tcgtggatgg
     1861 ggaaggggta tgtttcgaag accttcctgt aaatcgtcgc catctgcccg atgtcatccc
     1921 ttgttgcctc gcgcagttcc aaatcaggtg atttgaagtc tgcgacatca ccttcagatt
     1981 ccgacgcgtt ttgcgcagcc agtatcagca catgctccag tttttccctg ttttcaggat
     2041 gtctgcggct gcgatccgga tatcttccca tgaagactgc agcttcgcgt ccaccgtaat
     2101 atcccggtat tcccgcttcg aggcggtatc ccgcgtcgag gaaccccggg gccacgctgt
     2161 tcgggatctt tgcgataatt tttcccaggt tttcgctttc ggccagtgat tgcagtcgcg
     2221 gcagcacatg ttccgggtcg gctccgttcc agcgcatcac gtagacacgg tcgctgaccg
     2281 ggccgtactg aatctgtgcc ttgccgatcc gggccgtcct gtcgaatttt gtctgtctgt
     2341 gttccctgac ttcgtcagcg gtccgggtgg caattcgttt cattgttgcg gattggttca
     2401 ttcgttctcc tttggccgtc atccggttcg tacgtcgctg ctgcagcagg gatcgtgcgg
     2461 caacaggttc ggggatgcgt cgatcgcttc cgcgtccagc agcctgaccg tcttccgcag
     2521 cgcggcggca attgcgttct gttcatcttc cggcagacgc cggaatcgtt gtacaaaggt
     2581 ttcctgcagc atcggcgggg cctcatcgac gatccttccc ccggcttcca gcagcatcgg
     2641 gatgactttc cgtcgatcct ggctgctgcg tacacggtgg ataagacctt tttcttccag
     2701 cctgtcggca attccattga cggtactggg gctcaggctg atttcttcgg ccagcccgga
     2761 aagcgtcaaa gacgggttgc gctggatggc gtacaggcac agaagctgtg gcaccgtcac
     2821 gttgaacgac acgttgatcc tgtgggactg catatcgatc gcgcggacga tttttcttaa
     2881 cgaacgcagg atttcagttt ccctgtcagc cattcaaata accctttcga tacgaatcat
     2941 tcgtatagat actaacagcg aatcgacagc cggtcaacgg gccggtttcc cgcctgatat
     3001 acagcatcgc gcccgacaaa accgtcaact ggcgttttga cgacttcgac aacccggaac
     3061 gtggcgactg cagcgacccg gtttattcaa acgtgtccga cacgctttac gtcatctgca
     3121 atctgacgcc tgcgggtggg cagtacagca cggtcgtgac ggcgattggg gcaaatggtc
     3181 agaaaaaatg gaccgcgaca ctgccggacg gccatacagc cagccttccg gtaattgccc
     3241 tgcgcagtct gtcggaaacg atggccgacc tgtaccaagt cctgaagcgt ttttacggtg
     3301 gcgtgccttt cgattacggt cagtgcccgc ggaattactg atgtcgtttc gtcaccgttt
     3361 cgtcagccgc agcatgtttc acgccgcatg cggcatccgg atctcaagcg gatgatccga
     3421 gctggccttc aggtttcgaa gacttccacc gatcagttca gccagtttgt cggtcattcc
     3481 ggccctggtc gccatgaaca ggcgcagcag gacctctgtg ttgtagtcca tgccggcagg
     3541 ggaatcctgc cgtgattccc attgggacac cacggaatgg tttgccatgt tcaacgtcgc
     3601 cgcaaaatcc acctggcgca tccgcagata cgatctgatg aagccgacct ccgaaccggt
     3661 cagcctggcg ggcttgagag ccagaaggct aaaaaccttg tcctgaagtt gccccatgtt
     3721 gacgtccgga acctgctcac cggctcgacc agcgactcca cctcggccac cgacgagtcc
     3781 acgagctcgt ggcgctcgct cggcgagttc ctggggcagg tgttcggcgg catcgtcacg
     3841 gtgatcaccc tggcgctcgg cgggctgatc aagatcatca ccgccgtcat ctgggtggtg
     3901 aacgcggtca aggacgcctt cgtcatcgcc ggcacctgga tcggcgagac ggcggccaag
     3961 atctacctct ggttcaccga gaccctgccc aacgccatct cgtcggccat cgacacggtg
     4021 gtcggcttct tccgcgccat cgggcagttc ttcgtgggca tcggccgctg gttcaagaac
     4081 ctcttccgca gcatcgccga cggcatcatg agcttcctcc agccggtggt ggacttcttc
     4141 cgcggcgtgg gccgggccat caaggcggtc ttcgacggca tccgcgacct ggtgatccgc
     4201 atcctgcggc gcatcccgga cgcgctcctg ccggcgagcc tcgagcggct caagcgcacg
     4261 ccgctctcga ccgaggtgcg caccgaggac gagttcgccg ccgtgggtcg gaccgagtcc
     4321 actgccgcgc ggtccgaggc ggcgacgtcc tcgatgcctt cgagcgctga ggccaccggc
     4381 cgcatgaacg agttcgccca gctcgaggcc aacatgatgg ccttcgccaa ctcccaggcg
     4441 cgggatcgcg ggcaggcgcc acccttccag atcaacgtcc aggtggacgg cgagaccatc
     4501 gcccgggcca cccacaacgc gggctccgac gcagcggcga ggtcgttctc gccggtgccg
     4561 gcctactgag gtgacgatgg gtatcgagct cgccatggtc gctcctcccc gctgcgtgct
     4621 cgtcaacgtg accacgggcg agtcgatgga gtgcctgctc aacccgacgc agctctcgga
     4681 gaagctccag gtcaactgga accgcctggc cgtgcccggg ctgtcgcacc aggtgctgca
     4741 gttccagtcc acgggcaacc gccaactctc gggagtcgag ttctacctcg accgcttctt
     4801 cgccgtggag cagccgggcg agccggacat catggagttc cgcaggttcc tgcgggcgct
     4861 caccgtgccg cccgagggca ccgaggacgt ggcgagcacg gcaccgccgc gggtgctcgt
     4921 catctggccc gaggtcatta ccgtcgaggc ggtggtgacc gacgtcgagt tccagtaccg
     4981 ccaggtcggg gtggacggca cggtgctcgt ctacgcagcc accgtaggct tcgaggagat
     5041 cctcgacgcg cgggtgacat ccgaagccct gcgggaggag gtgtagccgt gccgccgcgc
     5101 gtgggctccc gccacagctt caccttcggt gtccacgacg ccgacggcgt gctgcacctg
     5161 accgagcgcg agccgtaccg gtacaaggcg cacaccgaca accgcgtcca cgtcgtggtc
     5221 cagggcgaca cgctctggga cctcgcgggc cgctacttcg cgccgctgcc tcgggcctgc
     5281 ggcttctggt gggccatcgc cgacttccag cccgacccca tcgtggaccc gacgctcggg
     5341 ctcgaagcgg gtcggcgcct cttcatcccc tcgctgcgcg tgctgaccga cgtgatcctc
     5401 ggcgaggccg gacggaggtc gcgcggatga acccgatcat cgaccgcagc gcccccggtg
     5461 tccgcatcac gctgctcgcc gacgagcgcg ccgagagcgg cgagccgctc gagctcggcg
     5521 gccgcatcat cggcttcacc ttcgacgatg ccgagagcaa agccgacaag ctctcgctcc
     5581 agctcgacaa ccaggatcta tcgctcttcg accgcgagga cctgatgggc ggggccacgc
     5641 tggaggtctc ctggggctac gcgggcaaca tggccgtgcc gcgccgggtg gtggtcaaga
     5701 agctcaaggg cttcaccacg ctcgaggtcg aggggcaggc cctctcgacg ctgatgaacc
     5761 gcgaggccaa gacccggagc tggcagaacg tgacccgctc ggcggtggtc cggcagatcg
     5821 ccgaggagca cggctacgaa gggcagttca tcgatgccga ggacaccggc gaggtgctcg
     5881 acgtcatcaa ccagaccgcc gagaccgacg cgcgcttcct gcggcggctc gcttcgtgcg
     5941 aggagttcga gttctacgtg gacgacggcg gctttcactt ccacgagcgg cggcaggagg
     6001 ccgctcccac ccatgtcttc acctggtact ctgatccggg acgcggcgac atcctatcga
     6061 tcaacgtcga gtcggatctc atcaagcgcg ctggccgtgt gaccgtgcgc ggccgcgatc
     6121 cgatgagccg ctcgaccatc gagagctcat cgagcaacga cactgccggc cggtcgactc
     6181 tggccgaggt ggtcgaggtg gtggacccgg aaacgggctc cacctcgctc gagacgcgca
     6241 atgccacggc gagcgtgcat ccgacatcgg cacccaccgc gggtcgggcg gcgcgcgagt
     6301 ccgcggccag gttccgccgc gccgagcggg cgaccgtgaa gctctcgatg caggtcgtgg
     6361 gcgacccgac cctgcacgcc aagagcatcg tcgaggtgcg cggcgtctcc gggctgctct
     6421 cgggcaagta ctacgtcacc ggggtcaagc acgtcgtctc ttcctcgggc tacacctgcg
     6481 agctgaagct cacccgtgat gggcagggtc gccgcgcccg gcaggtcgcc cgcgagcagc
     6541 ggggagagcg caacaccagc cgcccgcgca ccgacggcga gatgacccag gtcgaggccg
     6601 tggacccgga aaccggctcg acccgcatcg agtaccgacg cgatggccgc cgcatcggct
     6661 acgacgaccc cgaggccggg atgagcgtga tggagtagcc atgtcctaca gccaattcga
     6721 cgacgacatc ggcgcgcacg acacccggct gctgggcatg tacgtgggct acgtcaccca
     6781 tcgcaacgac cccgagaagc tcggccgggt gcgcgtgtgc atcccgggcg tgctcgagcc
     6841 gttcagcgcc tgggcctggc cgctcggcac ctcgggtggc ggcttcaagg accgaggctt
     6901 cttcgccgtg cccgaggagg gggccgaggt cgcggtcttc ttcaaccagg gcgacgtgga
     6961 cgcgccctac tatctgtccg cgcactgggg caagccggac ggcgagagcg aggtgcccga
     7021 ggaggcccag aaggacccgc ccgacaaccg ggtcattgcc acgccgacct tccgcattga
     7081 gctcgacgag tcggacaagg gcaagaagct caagctcacc aaccgcaaga ccggcgacca
     7141 cctgctcttc gacgccgagg agaacaccat cacgctcgag ggaaccacgg cgatcaccat
     7201 ccgcgcggtc ggcgccatca gcctcgaggc ggcgcaggtg accatcgccg gccgggccgt
     7261 gcgcccggtc gcggatccca tctgaaggag gcggccgtgg ccttgcccat ctgcatcgag
     7321 atccctgaaa ttcccgaccc cttcaacctg acgctgccgg gcgggattca gatcgagcac
     7381 atcaacctga tggaggtgat ccagcccgcg ctcactccgc tcgtgccgat cttcgacatc
     7441 atcgacaccg tggtcgcggt gttcaactgc atcaaggcca tcccggacac gctcggtccg
     7501 ccaccggacc cgaccgccat cgcggcttgc ctgccggacc tcgccgagaa ggtggccaag
     7561 ctcctcaagc tcatcccgca gctctcgatc ccgctcacca tcatcgggct catcgacctg
     7621 gtgatcgaca cgctgctccg ggcgcgcagc gagatcatgc acctgcagca acagatgcag
     7681 cagatcctcg gggtcatcga tcgggccacg aacctcgacg acgctggcct gatggccatc
     7741 gctcagtgcg cgcaggcgaa cgtggcgcag gaggcggcga acgtgggcaa gagcctggcg
     7801 gcgctcggca agctcatcgg catcatcaac ctgttcatgg gcatggtggg cgggcccgag
     7861 atcccggacc tgtcgaacct cgcgggccgg ccgctcgacg acgtggtccc gccgctcaac
     7921 gccatcgtgg acgcgctcaa gaccgcacgc gacgcggtgc cggtgccgta ggaggaacgc
     7981 gatgaacgac cacgagagcc agagccgggg cctgctggtc ccgttcagga gggacaagaa
     8041 gcgggacttc gcctcggaca ccggtgcgga cctactcggg tcgaaggtgc ttcaggcgct
     8101 catgacctgc ggcgctacac cccgatcctc gggcgagctg ccctggcgca cagcatttgg
     8161 cgccggcctc gatctcctgc gccaccagcg caacgacgcg gccctggccg agctcgcccg
     8221 ggtgcaggtc cgggacgccc tcaaacgctg ggtctccgag gccgagctgg tggaggtctc
     8281 ggtcagccga gaagacgctt cgctgcttct gcgggtgcgg ttccgtccag cgagacgagc
     8341 cggtcgtggt gacgaacagc ccggcgaggt gacgctggcg atcgaggcct gagcctgtcc
     8401 ccgcccgggc ccctccgggc tttgcccctt tggaggtgag acccgtggcc gtgcttccga
     8461 ccagcctcga ctacacggac aaggacttcg acgccctgcg ggcgcggctc atcgcgctcg
     8521 tgaagagcgt gtttccggac tggtccgact tctcggtggc gagcttcggc aacctcctca
     8581 tcgagatgta cgccttcgtc ggggacgtgc tcaccttcta cctcgacaac caggcccgcg
     8641 agtcgcgcct ctccaccgct acccagcgca agaacgtcat cgcgctcgcc cgcatgctcg
     8701 gctacaagct ccacggcgct caggccgcga ccgccgaggt gctgctcaag ctcgcgcggc
     8761 caccgtcggc ggacgtggtc atccccgcgg gcacggtcgt gcgcacccag gaggtgaccg
     8821 agccggttcg cttccagctc ctcgccgacg tcaccatccc ggcgggcgcc gacccgccgg
     8881 aggtgctgtg cgtggtcgag agctcggaaa cccgcaccca gctcttcgac gcccgggggc
     8941 tcgtcgatct cgacatcctg ctcgaccacg cgccctacct cgacggctcg gccgtggtct
     9001 ccgcggccaa cggcgcctac gtcgaggagg acagcctgct cggctccgag cccaacgatc
     9061 ggcacttcat ggtgctggtg gaccagaacg accgggccac cattcgcttc ggcaacggca
     9121 cgagcggcgc gccgcccacg ggcacggtgc agatcgccta caagaccggc ggcggcgcgg
     9181 gcggcaacgt ggacgccggc aggctcgtgg tcatcgaggg cgccttcgcc gatgtccacg
     9241 gtcacccggt gcaggtctcg gtcacgaacc cgtcgccggc aacgggtggt gccgaccgcc
     9301 agggcgtggc ctcggccaag ctgctcgcac ccgagagcct gcgcaccctc acccgcaccg
     9361 tgacccgcga agacttcgag gtcaacgcgc gccgcgtacc cggcgtggcc cgcgccttga
     9421 tgctgacctc gaacgaggac acctcgatcg acgagaacgc gggcatcctc tacgtcatcc
     9481 ccgagggcgg cggcctgccc acggtcgcgc tcaggaacct cgttctggct caggtcaccg
     9541 acgtctaccc gcacacgctg accttccagg tcgcggtgca gagcccggtc taccgcaacg
     9601 tggacatcga ggcgcggatc tatctgcgcc agggtcacgc caaggccgcg gtgcgcgacc
     9661 gggtgaaggc gaacctccag gccatgttcc gggtgagcga gccggacggc acgccaaacc
     9721 cgctcgtgga cttcggcttc aacatcaagg acgcggacgg caacccggtg ggtgagatcg
     9781 cctggtccga cgtgttcaac gtcatccgcg acacgacggg cgtgcgcaag gtcggcgacc
     9841 gccatggcga tctcaagctc aacaacctgc ccgcggacgt gcggctcggg atcaaggagt
     9901 tcccgctgct cggcaacgtg acgctcatcg acggcgacac ggggagctac ctctgatggc
     9961 cgtcgcgaac ccgagctacg aggacgcggg ggccgcgccg ggccaggccg agcactggac
    10021 gctgcggacc agcgcctcgc tcgagcggat cgccggcttc ggtcccgacc cgtaccgcgc
    10081 ctgggaggac ttcgagcgct ggttcgacct cgtgctctcg ctcggccccg gcgacgtggc
    10141 gatcgccttc ttcgacccgc tcgccgaggg ctacgaggac ttcgaggagg cctgggccaa
    10201 cgacctcttc atgaccgagc tgccgagcgg gcaggtcgtc accgcgccct tcggcggcgg
    10261 cgcggtcgaa gacctggagg acggctggga caacgtgtcc tacgcctggg cctgggaaga
    10321 ggtcgcggcc gtgaccggag tcttcgacgg cgagccccgc gaggacttcg aggaccagtg
    10381 gcgctcgaac cagagctacg cctggtcctg gggctcggtc acttcccagg cggcgatgtt
    10441 cgacggcggc acccagaacc gcgaggattt cgagaacgac tggagcgccg ccagcaccat
    10501 ctgaaggaga cgagcatggc cgaaaccgat tggacctacc tcaacgacgg gctcgacatc
    10561 gccaccgtgg atcggggcgt gaccgcaggc atcgcccggc cgccgggcgg tgggagcttc
    10621 cttttcgcct tcaactcgct tgcggccgtg cagggtgcgg tcgcactctt cgccaacctc
    10681 gtcgacttcg cgcccatggc caagggcgga tccatccgcg gctgcgtgca gcgcggtcct
    10741 ggcggcggcc cgacgggctt ctcgcccttt ctgtacctct gctgccaggg caactcggtc
    10801 aacgacacgg cgtacctgct cggcctctcc gacgatgacc cgcaccgcat cgtgctgcgc
    10861 aagggcatgg tcggcgccgg catcccggac tcggaggggg cgaacgtgct cctcgcctcg
    10921 ggtgagagct tcgcgcaggc gacctggctg cacctcaggc tcgacgcgat cgtgaacctg
    10981 aacggcgacg tggagctcaa ggtgttcaag aacgacctcg tcgcccatcc gctcggcacc
    11041 gctcctgact ggcagcccgt ctcgggcatg gccgatttca tcgacgacca cctcggtatc
    11101 aacagcggca gccagccgct cacctcgggg cgcggcggct tcggtgtgac ggtcaaggac
    11161 gtcacgcgcc gctgcttgtt cgaccacctc gagctgttga ggcaggtctg atggagctcg
    11221 acgccttcac gagccgcctc ggtctgggcc agggacggat cgcgccggtc aacgctgcgc
    11281 cggcctcggg cgaccacctg ttcgtgctgg gcgaggacga gcttggccgg ttcttcgacc
    11341 tcgcacccgg tgaccacgcg gaggtcgtgc aggacacgga cctcaccgac gtggccctcg
    11401 tccgcgccaa cctgcggctg cgcgtgccta cgagcctgcc gaccacgcac ggctgggagg
    11461 cgtcgatcgt cgtggatggc gtgaaggtcg cccgcgctac ctgccgcgct ggacgagagc
    11521 gcctgctcac cgacctcgcg gccaacgtct cgaagctgac cggactgcac gaggtgggcg
    11581 tgcgcctcga gctcgtggag gtgtagatgc cgctcttcga gctgcccgca ctctacgccg
    11641 acaacgtcgc ggccatcgtc acggtcgacc ggatcgtgct cgtgaaccgc gatccgggtc
    11701 cggccgagac gggcgtgccg ctcgactgga ccgtcgccct cgaggtgctc gacacgggac
    11761 cggacggcgt ggaccgctcg gcgactcgtg tctgggtgga cagcgtgctc gccttcgacg
    11821 gtggggccgc gcccgagatc cagcccggct tcgacgggtt gagggccgag gtcgtagaga
    11881 ccgcggacac cctgcgcatc gtgatcgatc ccgctgctcc gtgtgccagc gaggccgagg
    11941 tcgaggtgcg ggtggtctcc acgacggtcg gcggcggcca cgcgctcgac gagagctacg
    12001 ccttcttcgc cgaggaccgc accgccccca gggtgctggc ggcgcaggcg accagcccga
    12061 cgaccgtgca gatcggcttc gacgaggacg tggtcgtgac caaccccgag gggttcgtgt
    12121 tcgcgccgcg agccttcccg gctgtgccgg tgctggccgt cggagcctcg gcgcagggct
    12181 cggtcgtcac ggtcgagctc gacaccgaga tgacccccga cgtgtttcac gaggtcgtgg
    12241 tgtcgggcgt cgccgacctg cgaggcaacc tggtcctcga ggcccacgac cgcgcgttct
    12301 tcaacggctt caggcccgcg cgcccggcga gccggcgctt cgacctctgg tccatgctgc
    12361 cccggcacaa ccgccgtggg gacgcgaccg gagacctgcg gcgcttcatc gcctgcctcc
    12421 aggaggcgct cgacctggtc ctcgccgagg ccgaccgctt cgccgacgtg ttcgacatcg
    12481 agcgggcgcc cgagtccttc cttgacctca tcctccagga cctcggcaac ccgttcccct
    12541 tcgatctcga cgtgctcggc aagcggcggc tcgcctcggt gctcgtggag atgtaccggc
    12601 agaaggggac cgaggccggc atcgagaacg ccatccgctt cttcctcggc atcgacgtca
    12661 cggcgatcac cccgttcacg gggaccaccc tggtgctcgg cgagtcagag ctgggcgtgg
    12721 actgggagct cgggccctcg gaccgcttcg ctcgctacgc ttttgacgtc gaggtggacg
    12781 tgccgctcac caccacggag cgccgccaga tccgggccat cgtggactac ctcaagcccg
    12841 cccacaccca cttcgtggac ctgatcgagc caggagcccc ggtcgcctac gaccactggg
    12901 agctgggcat cagcgagctg ggggagacga ccgacctgca ctgacgtccc ccctgtcccc
    12961 gtccggcgcc cccgaggctt tgcccctctg gaggcagact gcccaaggag cgccggatgg
    13021 ctggacgaac ggactacttt tttcgccaga aggttacgga ggccgagctc gacctggcct
    13081 tcgagctgct cgagctggcc gaccgcaacc tggccgcgga tatcggggtc tacggcatca
    13141 tcagcggggc cgagcccacg ccccactcgc cggtcccgaa cctcaccgtc gacctgaccg
    13201 cgccggcccg ggcctacgac aacctgggcc agcgcatctt cttcggcacg ggccaggtgg
    13261 tggactgctc ggtggacctc accggcatcc ccaccgaggt gcccgtcgcc ggccaggagc
    13321 gctggctcgg gctgttcctg cgcttcgacc ggctgctcag cgacccgcgc accgacggca
    13381 actcccagca ggtgttcttc cggcgcgacg agtccttcga ggtcgtggtc cgccagggac
    13441 cgctggggcc ggtgggcggc gcctccaagg tgccggtcat gcccgacgaa ttgctcatct
    13501 gcgacgtgct gcgcaaaaac gcccagaccc agatcctggc tccggacatc gacgtgaccc
    13561 ggcgccaggc cttcatcttc gcccagggcg acgcggtcga gatcgtgagc agcgtgtgga
    13621 gcgtcctcgc gcccgccgtg gacacggtgc aggccgcact cgacgagatc gactccgagc
    13681 tggccggcca cttcggcggc tcggcacgac gccatccggc gagcgacatc gactacacgc
    13741 cccacggctt cgtcgccgcc ggcaacgtgc aggctgccct cgatgaggtg gtggacgacc
    13801 tctcggcgtc agccgccggc aacccgggag cccagcgggt gggcgcggac gcggtgagcg
    13861 gcacgccgca cgctctcacg gcaagcaacg tggacagcca cctctcgcag ctcctcggtt
    13921 ggctcaacgc ccatctcgcc gccgcgtccg gtgcgcacaa cgcgagcgcc atcgccgctg
    13981 ccgttcatag ctacatcgcc ggcacgaccg tccaggcgca gctccaggag atcgtgagcg
    14041 acctggccct gcagaccgcg ggctccgcgg gtgcctcgcg catcggcgcg gacgcggtct
    14101 cgggcagccc gaacgccctc gccgcgggga ccgtcgacag ccagctcgcg gagctgctcg
    14161 ccgacggcaa caaccacgtc ggcgcgacga gcggtgcgca tgcggcctct gccatctcgg
    14221 tcgccgactc gggcggcaac ctcaccgcct ccaacgtgga gaccgcgctc gccgagatcc
    14281 tcgacgcctt cgaggacgac cactaccggg ggaacgagag caacgctggc cagcaccgag
    14341 cgatccgtca gcccaacttc ggctcgggca aggtcctgct ctgggacgcg gtcggagtcg
    14401 gatccaacgc ctcgcacttc cgcgtccacg ccgacagcga ctcgatctgg ttcacggtca
    14461 acgccttctg gaacggcagc gcttggcagc gtgacctcgc cggcaactac gccggcggtt
    14521 tccgcttctc gcggaccgcc ttcgagatgt tccacgacaa cagcttcacg ctgaccttca
    14581 ccgactggac tcggacctgg cgcttgccca tgagcggcac ggtcaactcg gccttcgaga
    14641 tgaccggctc gacaaccgag gtggggcgtc tcggctacga gatgcacaac acctacaacg
    14701 ccacgcggaa catggcggtc ggtggcgcga acaccttccg ctcgcgcttc ccgtccaccc
    14761 cgtcgtcgat cacgttctcc gagaccagca gctacaacac cgacggaccg ccgggcctgt
    14821 gggacgccga ccgcgatggt ttcggcttct tcgactacgt caacgtggcc gccaactcga
    14881 ccctctactg gttcggcacc tacaccgccg tcgcctgagg agcagctatg cccattcgtg
    14941 agatcagagc cgacgacttc gaccaggagt gcagcaagtg cggcagcatc gtcgccgtgc
    15001 cgctgaccgc catcgcggcg ggcatctccc gcagcgggca gatgaacccg aacgtcgtgg
    15061 ccatgccggt ctgcgcgacc tgcggcgcgg ccgagttcct gctgcgctcc cccggcgagg
    15121 aggagcaccc ctcccagggc agctacggcc acaagcacaa gctgctcgtg gacaagctgc
    15181 acgccaagct cgtccagcaa gggcgggtcg cggaggggct gacgcccgag caggtgccga
    15241 ccaaggagcc gcccgccgcc gtcatggaca agtggttcaa ggacggcctc aagctcccgc
    15301 cgccgccgtt ccaggacgag tccggccgca gcgagccgaa gtaggaggag gccggtgaac
    15361 actcgtacct ttatcgtcgg cggcaaggag atcgagccgc cgtccggtct cgacgtcctc
    15421 aacttcaccg acgcgagcgt ccaccgcttt cgcggcaggg accgcacggg caagcccgtc
    15481 accgagctcg tcatccacga gacggtcacg cgctcggccg ccgacaccgt ggtcgtgctc
    15541 aagaagcggc ggctcggcgt ccatctcatc ctcgacgagc acggccgctt cacccagcac
    15601 ggggacctcg ccgacgaccg gctggcgcac gcgagccagc acaacggccc gtcgatcggc
    15661 atcgaggtcg tcaccccgta ctacccggcg tacctcaagc ccggcatggt ctggtcgact
    15721 gtcatcgacg cgccctgggc ccacaagaag aagtacgtgc tgccgacgcc ggtccaggcc
    15781 gaggcggtcg cgcggttcgt cgagtgggtg acctcgcccg cgtcgagcct ctccatccca
    15841 cgaacgtgga tcggtcttca cggcaccgcg ctcgcgatgg gccagatcga gagcgccgcc
    15901 aagctcaagc ccggcctcta cgcccatcac tacttcggac acgccgacgg cgcctggctc
    15961 gtgctctacg cctggcttcg cctcgaggcc ggcctcgctc ctgaggtcgc cttcgacgaa
    16021 gcggcgcggc gcgcgaccgg cgtgcgcagg cgagtggatc tctcggacct catgacggcg
    16081 gccgcgccga aggagtagcc catggacccg agcagcttca caaccatcgc cgaggtcttg
    16141 aaggccaccg gtccgtacgg actcgttgcc gtcttgggct gggccttctg gcgggtgaac
    16201 gagaagaagg accgcgagct gaaggacctt cacgcgaccg tggtgaagat ggccgaggcg
    16261 cagaccgccg ccatcaccaa ggtcgagtcc gcactggtcg cgctcaagga agcgatccgc
    16321 gagatgcgcg cggggtgagg cgatccgggg accgctcctg gtgcgatttc tcgcgcccct
    16381 cccggcgacg tcgaatgtcc cgctgccact tcccgcgtga tttccgcgac ctgcgcatcc
    16441 gtttcaaccc atcgaaaata cacgtgaaat cgacagaaag accttgaggt tctgcccgcg
    16501 caacgcctgt atgtccatga cgatcgagat gggctcgatc gcgagcgcgg aggcgaacga
    16561 tggcgaagat tctcggaagc acgacgacct accacggaac ggagcacccc tacctgaagg
    16621 gccacaaagt ccgcatcgtc gcggtgctca agaacgcggc ccggcccgac atcgacgtcg
    16681 acggccccga ctacgaccac atcagtgatg aagacgacct cgcccgcgcg ggcggcgtca
    16741 ccgccgacga ccggatcgag gtccagccct ggatccagaa ggagggccgc ttctccttcg
    16801 tgaccagcga cccccgggcg gtggacctcg cctacttcgc cggcgcgaag aaggcagcga
    16861 agaaaggggg ccagcgatga ccaccagctc gaagcgctac ccggtccagg tcgaaggcga
    16921 gatcaggtac gtcaccgtcc ccggctcggg tgacccgcag cccggtccgg agagcgagga
    16981 cgaactcgag agcttccttg ccgaggccat cgaggcctac gcggaggcga gcgaggtctc
    17041 tcggccgcgg atcagcactt tcgccgacgc cggcgtgctc accgccaacc gcggcctggt
    17101 ggtgcgcatc gggaccgccg agttccaggt gaccctggtg aggtccaggt gaacgtcgcc
    17161 gcgcagcgat cggagtcaga aacccgccgg tgcggaagct aagcaccggc ccagaccgaa
    17221 cgaggaggac gacatgaaca aggagatcag gaagcaggtg aaggccctgg agaagctgcg
    17281 gctgcccgag ctgcgggccc gctacgccga ggtcctcggc gaggagacga gggcgccgaa
    17341 tcgcacctac ctcatccggc gcatcaccga ggcgctcgag gcgcgggctg cggaagcgcc
    17401 cgagccggcc gaggccgagc cccaggcgac gcccgtcgag acagccccca cggagacggc
    17461 ggcggcgagc ccggagacgg gcgacgccac cgagaccaag ctcagcaagc tccccatcgc
    17521 cgagctgcag gctcgctacc tcgaggtcgt ccaaaggcca actgcgtcgg tgcatcgccg
    17581 ctacctcatc tggaagatcc gagaggcgca gaagggccgc atcccggtcg gaccgcaggg
    17641 cagccgacgc gccgacggcg aggcgctcga cttcaaggtc ctgccgctgc gcatggaggc
    17701 cgacctggtc gcgaagctgg acgaggcccg cgagcgcctg ggcctcaaga gccgcatgga
    17761 gctgctgcgc cggtccatcc acctcttcct ggcccgcgag ggcgaggccg aggtcgccgc
    17821 cctcttcgcg ccggaggcgt agatggccgg cgactacgac aagcgccgcc tggtcgagtg
    17881 gctgcgggcc gagatccaac gccagaccgg gcggcgctac gaccgcctcg acctcgactc
    17941 gctcgacccc gtgagcctcc gcgagctcca gcgcctcctg cgcgacctcg acgccgagca
    18001 gcggatggcc atccagcgtg cccggatctg cccctggcgc acgccgtgag cccgcccccg
    18061 ggcggaaacg gccgatcccc tccggccgta acttggcgga accttgtcac tactcaagtg
    18121 cttgaaaata gtgttctttt cctcgatttc gacctggact tccccgccga aggaagcctg
    18181 tatgtcctca ggatgaggac gatgcaacac atcgaaaacg aaggagaaaa cgcgatgagg
    18241 acgctccgct tcggcatcga gatcgagacg gtcggccaga cccgcgagcg ggtggcccag
    18301 gccatccagc gggtggtcgg cggcaccacc agccacgtgg gcgcgccctt cagctacgac
    18361 ccctggcacg tcaccgacgc ccgcggccgg acctggaagg tggtggccga cagcagcctg
    18421 aacgccagcc gcgaccgcca ggccgaggtg gtcagcccgg tcctgaccta cgaggacctg
    18481 ccggaactgc aggagatcgt ccgggccctg cgcgccgccg gcgccaaggc cgacagcagc
    18541 tgcgggatcc acgtccacgt cgaggctgcc cgattcggcg ccaagggcct gcgcaacctg
    18601 gtcaagatcg tcaacaagca ggagaggctg atcgagcacg ccctcgggat cagcgaagcc
    18661 cgccgcgccc gctggtgccg gggcatcgac caagccttcc tggacaagat cgagaagcag
    18721 cgcccgcgcg acctcgagga catcaaccgc gcctggtacg gctaccacaa caccgcgccg
    18781 atccactgcg acgcctcgag gtaccacggg gtcaacctgc acaacgtctg gttccggggc
    18841 acggtcgagt accgctggtt cgacggcacg ctccacgcgg gcaagatcaa ggcctacatc
    18901 cagttcgtcc tcgcgctcag cgccaaggcc atgcgggcgc gggcgagcag cagcaagcgc
    18961 cgcgagttca acccggacac ggcccgctac gacttccgcg tgtttttgct gcatctggac
    19021 ctgatcggcg acgagttcaa gaccgcccgg ttgcacctga tggcgaacct caacggctcg
    19081 gcagcctgga agcacgggcg gcccgagcag cagccggcct tgtgaaagga ggcggcacga
    19141 tgaagcagac cctgtacttc gcctacggct cgaacctgga tacggcccgg atgcgccgac
    19201 gttgccccac agccgcgccg gtcggcccgg ccacgctgtg gggctggcgg ctcgccttcg
    19261 gcggacacag ccgcgcctgg ggcggcccgg tggccaccgt ggtgcgcgcc gcggacgagc
    19321 gggtggacgg gctcctttac gagctggccc tcggggagct ggccgacctc gaccgcgtcg
    19381 agggacaccc gctgagctac cgtcgcaagc gcctgcgcgt ggtcgatggc cgggggcggg
    19441 agcgccgagc tcacgtctac atcctgccgg tggcatggga agcgcctccc gcgcccgact
    19501 acctcggcgt gctgtggcac gcctaccggc gcctcggctt cgaccgcgac tccttgctcg
    19561 aggcggcggg aggtgcggga tgagcaccgg gaccaacggg aagaccagag tgttcgtcta
    19621 cggcacgctg ctcagcggcg agcccaacca ccgcctcctg gccagcgccg cccgggtggg
    19681 cgaggccagg accgagcccg ccttcgacct ggtgagcctc ggcgcgttcc cggctatggt
    19741 ggccggcgga cggacagcgg ttgccggcga ggtgtacgag gtcgacgccg ggacgctggc
    19801 cgatctcgac cggctcgagg gccacccgcg cttctaccag cggcgccgga tccggctcga
    19861 ggacggcggc gaggtgctcg cctacgtctt cacctccgag caggcgcggg gccggccggc
    19921 gatcgcgagc ggcaactgga gacacgaacg aaaggagaga tggtcatgag aatccgaatg
    19981 ctacgcgacg ggcgcgtctt ccagggcacg cccaagcaga tcgtcgaggc gatgcagtac
    20041 atcgccttcg gccaggagaa ccgaaccctg ggcgagtaca tcgactggct cgtggaccag
    20101 gtccagcggc tcgagtcgat tgacctcaag gtcgagggcg acaccgacga ggacaaggcg
    20161 gcctccctgg tccaggccat gatgggcagc gggctcgcgg agaagatgta gaccaggacc
    20221 gggaacgctg gacgaccagg catcatccgg ccgatgtccc tgtcaaccta gcgagagggt
    20281 gacccacggt gtgggcgtcc attcttgggc cgcctggagc ctgcccgcca cacgtctctg
    20341 ctattcctcg gagtccaatg ttgcgacctc attgaagatt cggcgcaatg tacttgcgag
    20401 cggttcgcgg tgagggtaga ttggggagcc aatcaaagtc gcctaccaac ccggatgggc
    20461 ttggtggttt acaggagccc cggtcgacaa cgaccggagc ttcgtcgtag aagggccagt
    20521 tcttgcgggc gtttacgctt cccgactctc gtcgtctgct ctcgccttgc gatcaggaag
    20581 cgcatcgtcg aggccgcccg ggctcaggtg gcgaggtccg tcaacaccgc gatggtccac
    20641 gcgtactggc tcatcggccg cgaaatcgtc gaggtcgaac aggagggcaa ggagagggcc
    20701 gactacggcg aacaggtggt ccgacggctg gccgagcgac tcggcaagcg gttcggcaag
    20761 ggcttcagct acccctccgt caagcggatg aagcagttct acctgtcgtt cccgcagggc
    20821 tcggccatcc ccgaggggct gggcggctat cagaaaggct cggcgacgct gagcctttcg
    20881 ccgggctccg acgtaatcga aaaaggctca acgctgctga gccaatctgc gcgtcacgtg
    20941 tccacgctgt tcccgcctct cctgtcctgg tcgcactacc tgccgaccga ggagcagctc
    21001 cgcgaggacg aggccgagca ccggatggcc gttcagcggc tcggatctgc ccctgtagat
    21061 cttgaggggg ttgagccctg catcgtatcg gtggtcggtc gtctgccact acgacaggaa
    21121 tcgcttgatc aaagcaatag ctacccccag cactcgggcg gcatcgacaa catcgccacg
    21181 gtcctcgccg agctcgccga gcagctggac cccgccgccc tagcttcgct cgctcctcta
    21241 tctcccgtgc cctgggcgca gcggcttggc catctcctag agcgggtcgg tgccgctgac
    21301 aaggcgagcc cgctcgcaga gcacgtcgcg agcgtcgtga acgagacgac cgcgctggtt
    21361 gcgagctcgc cggcagaggg agcgccgtac gacaagcgct ggcggctgct ggcgaatgtc
    21421 caagtggagc ccgacctatg atccccctcg acttcatcac cgaatggcgc cgtacggctc
    21481 cctggatcca cgactcgcag gtcgagcagg acctcctcgt gacgttcaag accgataacc
    21541 ctggctgggg agccagccta tgcgccggtt atgctctcgg tttcgcgctc gcgaccgttt
    21601 ggaggtgact cgactccagt tgagttaggg ggcaagtgga gtgtcccctg cgctaattac
    21661 attcattcgt ctgttcatta cagccatcag gacaagtata ctgctttttc cattcgccat
    21721 gtgtacacaa atagacccag aacctatcat ccccaccccg tttctcacat accttttcgt
    21781 cattgaggca cgttccgcag acggtcattt ctgattcgcc ttcgcatgaa tatttcaggg
    21841 atccattatt gttttggcat ttgtctccca gaacgatctt tccggcgaca cacgtttcga
    21901 gatgtccggc ctgtgtatct tcgtcgtttt tgcagatgtt attcgagtgg tttttgcact
    21961 cgccgcattc gtttgtcaca acgtcgcatg aaacatcatc acattggttt gcatcatcct
    22021 tccagacgcc ggcgatgcat gtcctaggtt tccctatttc attctcatca tcgatacatt
    22081 gcttgctctc gtttttgcac tcgccgcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22201 nnnnnnnnca ctcgccgcac tccgtttcgg atttgcacga tgcgccgttg tcgcaatttt
    22261 cttcgccgcc gtaaacgccg ccactgcatg tttttagctt gccatcgaca cactgggtgt
    22321 ccccgtcatc gcaccgtgtg gcacatgatt ccttgtcttc cgcgcatccg gcatcgcaga
    22381 cttcggtggt agtccatgtt tcgctcgtgc atgtctgcaa tttgccatca aagcacgaaa
    22441 catcgccttc ggtacaagaa agttcgcatt tcaagccgtc atccgtacat ttcccagatt
    22501 tgcatgcgtc cccggcgatg atttctccgt cattgcatgt gaacagtctg ccatcctcac
    22561 agccatcaga tgtgcagttc gcacatttct tttgatcgga atggcagcct ttgtcgcacg
    22621 tttctccttc tttccaggta ttatcttcgc cacatgtgta catccggctg tttgcgcaaa
    22681 agacatcagc tgcgttgcag aagccggtcg gttttgattc gggacattct ttggtcaaat
    22741 aattgcatac aagccctgca tcgcacttta cgtttttgca ttcacagcgt tcgtccttgt
    22801 atgtcccccc gctggcttcg cacgacagtt caaatgcggt ttttgcgcat gcgccgtctt
    22861 tgcacaccac gcctgcagga cagaacgtct gtccgcagac gcataaatca ttagcaaacg
    22921 tgccgccgct atcttcacat ttcaatttgg ccgctgtctc ctcatcaagc ccatcgatgg
    22981 gacacggctc cgacgaaccg cagccgccgg tcaaaaaaat ggaaagcata aagaatgctg
    23041 cgataccgaa agctctcttc atcatgatct ctcctttggt ttagctatag gaactttcat
    23101 cccgcaaatc caccattctg gcaagtattg tcaaatctcc ctggagtgtc aattctgagt
    23161 gcgcaagcat cggtatccgc acgaaaatcg tctcaaactc tcgcacaaat agcatgctac
    23221 ctgcagagga ataggcgcct gtgagcgaaa ttcaagcagt ctttgcggac ctcggcctcc
    23281 gaggccgcag aggcgatcat gaggggggga ttcggtacga caccccttga ctgcttcaat
    23341 cctgctgccc accattgtat aatcgggcgc tcggtttttg ggctgggctg tgccctgttt
    23401 tttgcttgtt gctaacactt gagggtgtga tgaaacgtat cattctgttt ttgttgacta
    23461 acttggcgat tatgtttgtg ctttctattg tgctgggttt gctgtctacc cagcttgggc
    23521 ttgatccgac cggtattggg gcggtggtcc tgtttgcctt ggtgtttggt atgggtggag
    23581 ccctgatttc tttggcgatg tctaagttca ttgccaaaag aagtactggg gcacaggtca
    23641 ttaccaatcc caggtctgat acggagcact ggcttatcaa tacggtccgt gagctggcca
    23701 gtaccgcaaa tattggcatg cctgaggttg cggtttatag gggtgcgcct aatgcctttg
    23761 caactggtgc gacccgtaat aactcgcttg tggctgtgtc agaccagttg atgcaaaaca
    23821 tgactagggc cgaagtccgc gctgtgctcg cccatgaagt tggacacatc gctagtgggg
    23881 atatggttac catgaccctg cttcaaggcg tgatgaacac tctggtgctt ttgctggcca
    23941 gaatcatcgg tgtgaccgtt gataaagccg tgttcgggac cagaaatggg cgcggaattg
    24001 gctatttcat cacgtatatc gttatgcagt tagtgctggg gattctggca tccatcgtcg
    24061 ttatgggctt ttcgcgacgc cgtgagtttg ctgctgacgc caccgcggct aaattgttgg
    24121 gctcgccaag ggatatgatt gccgcgttgc aacgactggg ttccttggag cctggcgcct
    24181 tgccccagtc aatgaaggcc tttggtatta ccggcgcccc aaaatgggcg gcactgtttg
    24241 cctcccaccc tcccctggaa aaacgcatcg agcgcctgga aagagcctag tcccaaaatc
    24301 caaaacagga ttcaaaactt caagccgcca ccatcgctag caccgttgcc aacactcgca
    24361 cggaccaatc ccaaaatcca aaacaggatt caaaactcca agccgccacc atcgctagcc
    24421 agccaccacc caaaactttt cagctactta ttagacaagg tatggtcaca agcacccagt
    24481 tcgcctgcat acaagcaccc agttcgcctg catttcctcc cctcagggca cggtccagca
    24541 gcaccccact tagttgagca agtgcatcct atttcttgat cgcacacatc tgacctgtca
    24601 tcacaaacgg aagaccccga ttcatgcagg caataacaac caccaattcc attgcagtca
    24661 ggatttaggc gatttttgca ttgatatacg gtccctgtac actgcttgtt tgcatcgcag
    24721 gcatcactgt gggtacacat gttggcgtcg ttgcagggcc ttccaactgc aggcggatac
    24781 gagcagctgc ggtcctccag acacttcgcc ccaaccatag tttcgcaata ccctggcgca
    24841 tcacagtccg ctgcatcagg gtctggataa tataaacagt cgccattacc caaacaagcc
    24901 gcacccgctg cggtctcgca aaaaccagga tcttcgcaag tataagcaac gccagagcat
    24961 gttttgtctt gcttacatgt atcgttatac gtacataaat cgccatcatc acagggagca
    25021 tctgtgttag gcggatagta gcaggtaccg tcgccgttac acatggcacc aaccgcagtc
    25081 tcacacgccc caggtaaact acacgtatag aagaatcctt ggcagacggg caccccacct
    25141 ggtcctgcct tgcacttgtc attccaggta caagggtcat gatcatcaca ctctgcttcc
    25201 gaatctgggg ggtatttaca ggtgccgtca ccgttgcagg ttgcaccaac tacagtgccg
    25261 caatatggag ggttgtcgca agaatagtca actcccacac acgaaccatc tagctgacac
    25321 acgtcgtcaa aggtacaagc atcgccatcg tcacacgatt cccaaaccgc gatctcgtat
    25381 gtacaagtac cgtccccatt acaaatggca ccatcagcag tctcacataa acctggttcg
    25441 tcgcatgaat aggaagtccc caagcagatt ttgttgggtc tgcagttatc gtcataagta
    25501 caaggatctc catcatcgca cgccctattt atgtccgccg gatagttaca ggtaccatcg
    25561 ccgttacaga tcgctccctc ggacgtttcg caaaaccctg gagaatcgca ttcgtagagt
    25621 gttccaagac agacaggcaa gccatcttgg ccctcctgac agtggtcatc aatggtacac
    25681 ggattggcat cattacaggc ttttccctgt tctacaggat agttacaggt gccatcaccg
    25741 ttgcaggttg caccttcagc tgtggtgcac tctggaggct tatcacacat gtagaaagtg
    25801 ccccgacaag acttgttatc ctggcattta tcatcatagg tgcacgccag ttcatcgtcg
    25861 cacggggcgc cggcatcagc tgggtaatta cacgtaccat cacccttaca agttgccccc
    25921 tcggccttct cacaaaaccc tggagtttca catgcatatg cggttccagc acaaatgccg
    25981 tttccgaagc catcatccat gcacacatcg ttaaaagtgc ataaatcctt gtcgttacag
    26041 gcagcaccaa cgtctacctg ataggtacaa gtaccatctc cattgcatat agcgccaact
    26101 ttggtctgac aaaaacctgg ggaatcgcat tgataggttg agcccctaca acttttatcg
    26161 gcctgacaaa catcgtttat tgtacagggg tcaccatcgt cgcatctaac tccaacagcc
    26221 gccctatagg tgcaagtgcc atcaccattg caggttgccc cttcggcagt ttcacaaggt
    26281 ccaggcgcat cgcaagaata gcgagtccca atgcattgag ggcctcccgc cacatcggat
    26341 cggcactgat cgtttacaga acaaggattg ccatcatcac aggggtgtcc ctcattgaac
    26401 gaccattcag ttggatgcgc tgggtcacat acaaggcatt tcccctgtgt tgtttgagcc
    26461 ctatgacaaa caaggtcaat caggcagtag ttctttttga ttctttcaag gcagcctcgg
    26521 acggggtcac agtagttgtc agtacagaca agccagcctt cgcaattctc gtcggggccg
    26581 ctgggcacac aagagccttc aacgcacttc tgggctaata aaaacgtcag cccgtcgcac
    26641 ctacttcctg ggcaatcccc acattctcca ccgcattggt cagggccgca ctccttgcca
    26701 tcacacttag ggacacaatt cttgcatcgc gcattggcac aaccagagtg acactctgca
    26761 acgacttcac cagcttgacc gcaagagtca aaccagcaga cgttatcacc acaacagtcc
    26821 ttatgatgct tgggctcgca agtacaaacg ccctctatac agatttcacc accatcacat
    26881 tcgccacacg tgcctccaca accgtctcca ccacaattgc gaccctggca ctctggcaca
    26941 catacctcat catcacgtcc ctgcgttaac tcatcgaggt cagtaatgtc gttgtcatct
    27001 gagtctggcg agaccgcctc cgcgccaacc ctgtcagact cggcgtcggt gtcacggtct
    27061 ccaaaattaa actctggttg accataatca tatccttcgc cgtagtccct gcggtttgga
    27121 tccgaggaac caggctccaa tgtcgggcca gtcccacctc cacatgctga caggatcgat
    27181 aacactgcaa atacgggtac aagcctatgt gtcatgattc acctctcagc acggcatgaa
    27241 aacgcctttt aaaatggtct aaaaagtagc agcacacaac catttttcca aaattactag
    27301 cgccatcatt aagactggca tatccaaggg taatgtcaag tccaaaatcc ccaactggca
    27361 ttatacaaaa ttctgacagg gaacgcttgc ccaaaatcac ccaatgattc gcgcagtagc
    27421 cgtgattttt tagaccttcc ccactgcaat aacgttcgat ttggtcataa tcgagcgcat
    27481 acg
//
LOCUS       DAOTPW010000041        27470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1827_length_27470_cov_5.157943, whole genome shotgun sequence.
ACCESSION   DAOTPW010000041 DAOTPW010000000
VERSION     DAOTPW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27470
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1827_length_27470_cov_5.157943"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..784)
                     /locus_tag="P5316_05645"
     CDS             complement(83..784)
                     /locus_tag="P5316_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR84476.1"
                     /translation="MKLSIVVPVYNEVRTLEELVRRVLAVDVGMEKELILVDDASTDG
                     TRDLYDRIRREHAGQHIQVLMHPHNRGKGAALRTGFDKATGDIILVQDADLEYDPRDY
                     PRLLAPILDNRADVVFGSRFVGGEAHRVLFFWHMLGNKILTLLSNMLTNLNLTDMEVC
                     YKVFRREILRNFELKSDRFEFEPEFTAKIAKTGCRVYEVGISYAGRDYSEGKKITWVD
                     GIKAVIAIFRYSFFR"
     gene            1274..3853
                     /locus_tag="P5316_05650"
     CDS             1274..3853
                     /locus_tag="P5316_05650"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRR84477.1"
                     /translation="MTKGHMSKELIILAASLVFTTLPGAGEVKGQVPPGVAPVPPASS
                     VAPADPNGPKLRCDENTFDFGDAWSGTVVEHAYVIHNDGKSPLELLNVKTTCGCTVVD
                     YDKVIAPGGQGKVTVKLTLSKAVQSQPVSRSVQVESNDPANKVVPLTIKGKVKPRVAL
                     EPSNAVFGTVTDETDVTRVIKVTNYTEGHMKLEPVPLPPGQQSVFKAEVTEVEPGKVA
                     EIKITGVRPFAERNNSTVLQFTTGLDKDERISVPCSLYSPPLLEVSPNRLPLFLPLTR
                     EFIRQVTINYSGKEDFKIESVVPGNDQIKTELTPVAGSKSFRMVVRVPQDFTPPNHDP
                     IDIILKTNLKEKSELQVTLEPRVSPTAPQPQPPPVVVARVEDLLGKPTPVTSQMPLKA
                     GQAVQFGTGNQPAVVNFWAAWSSQCRRQLPYMRTLQSLYGVKGVTFLNISLDSLKPVG
                     EVLEAAKEAGLTEAALAADPQYTTAARFGIKRVPTVLLIGRNGTVEAVHEGVPAAEQM
                     AEYEEMLRNEIDALLEGKGRSEFGSIPRPAGSAELLEPQGAGTTPLGRMPRLTVESAR
                     QDLGGVKPASKVTAKLYYRNDGMEPLTINSVTASEGLSVGSEYTKSLTPGAMAVLNCT
                     FESGARPGPFEHKVTLTTNDPTRPKLDVVLLGTVRPYLECDPATGIDFGRHPRKHTMG
                     RLATILYHGTDEIEYLSAECTSPKFEVIVEKLRGSQNAKLVVNPKPPFDLGELKATIK
                     VTTTCKEQPTLEVPLRLYNPKRIEVTPEAIVIGGPARAQRFSVTIANNGLDNLSIFGI
                     TRSNNLIRARIYPDADDVSYKLDVFVPPTYVPPAEGDKITIRTDDKEFGEIVIPIKPA
                     AGG"
     gene            complement(3997..5697)
                     /gene="hcp"
                     /locus_tag="P5316_05655"
                     /gene_synonym="priS"
     CDS             complement(3997..5697)
                     /gene="hcp"
                     /locus_tag="P5316_05655"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRR84478.1"
                     /translation="MFCFQCEQAAKGTGCSTIGVCGKTPDVAALQDLLIHAAKGISMH
                     AHRAGYLGARDRQVDRFIVEALFTTVTNVNFSPERMGEELHKAASIRDRARILYEQAC
                     IKAGKQPEILTGPAIWSPAGDIDGLVAQGQDVGIMDRIRVRGMDVAGLEELLTYGLKG
                     TAAYAHHAMILGHEDDGVYAFFHEALDSLTRTDHTAASLLSLVLKCGEVNLKVMELLD
                     RANTSAYGHPVPTPVRITPVKGKAILISGHDLKDLEELLKQTEGTGINIYTHSEMLPA
                     HGYPELKKYKHLVGNYGGAWFQQQDEFERFPGAILMTTNCIQKPRDSYRHRLFTCGLV
                     AWPGVTHITDRDFRPVIEAALAADGFRHTEPEKTILVGFGHNSVLGAADKVIKAVKAG
                     RIRHFFLIGGCDGHKEGRNYYTDFAEAVPDDCVILTLACGKYRFNKLDFGTVEGLPRL
                     LDVGQCNDAYSAIKIAIGLADALKCGVNDLPLSLILSWYEQKAVCILLTLLHLGVRNI
                     RLGPSLPAFVSTNVFAVLSEKFGIRPIGTARENLAECLEATPSKTVPIVESDPGKTVG
                     "
     gene            6259..9066
                     /locus_tag="P5316_05660"
     CDS             6259..9066
                     /locus_tag="P5316_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRR84479.1"
                     /translation="MSRKLHSQAAMCVVVFLLPVAVMGEELPPEDSYDPGAGYYSAGY
                     QPLMPVFEEAAAEYDVPLDLLLALGKVGSAFEDRGTEPTIEGGYGIMALRDSEYSDSL
                     KQAARLLKLDSEVLKIDAAANIRGAAAVLDRAAKKAKIDRNQGLRAWLPVVIEYAGLD
                     AESSKFFARGIYQRLQNGFSKVNSAGEVFEVLSQSVGVDLDSLVPKGLVAVEATTFDY
                     QIMSAPGDCGNPDYALATWDPAPTCNYSTSVTNKDTIVIHVAEGSAAGTISWFKNCSS
                     GVAAHYVMDEAGAIWQMVCERHVGYHIVCYNSRAIGIEHEGYTADPSHPEAMYQASAN
                     WARDVCNRWGIPKQKARSAPGIVGHADVSECVRCTDHMDPGHGWDWGHYMALVNQAPD
                     PITFIVESRAGGKNNANYSEVGSFGNSSGKSTVWDCTSGIGSRYAYMNGTNRCSYSFT
                     PTTSGTYRVYVTWCTSANATQQLEHIVTHAGGSTSIVLDTDVDTNPCGRNNWNLLGEF
                     VLNSGVQYSVTQTSQNYHDGMVLRADAVKWEYVGGGGGVQPPTITQHPAAQSVCPGST
                     AVFSVTATGEGTLTYQWQKNQVNLTNGGHYSGVTTEALTVSNADGNDAANYRCVVSNA
                     GGSTTSNAAGLTLKAATTITQHPSDQTVATGGTANFSVAAAGDGALTYQWQKNQVNLS
                     NGGHYSGVTTATLTVSNADSNDAASYRCVVTAGCGIATSNSATLTVTTGGTTIFFDNF
                     DSYANQTAFTAVWPISVSPGGTLSTALYFSSPKSIYIGTSAARNQRNFTETTASDAVP
                     IVWKFRLYDNNTSNLDRQWCELLDASPSVVQLVALGKSNVQEAMRTYYAARVAYSPGP
                     GWVALNGPGAPTRSVGWHELKAVIKSTTIDFYVDGVLAKSGVSYANSQGQFSFDRARL
                     GSGYSSTSLAYYDDYSVVSGQ"
     gene            9180..11891
                     /locus_tag="P5316_05665"
     CDS             9180..11891
                     /locus_tag="P5316_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRR84480.1"
                     /translation="MSFGLVSRPVRIAVILAALPVGRAAAQTTEFRGMWADVWHEGYK
                     SAAQIDAMVARAVQGGYNAIFPELLAYQDSGSSAHGAYWNSAIVPKATDIQGGIDPLA
                     YLIQQAHAQGIEVHAWLTAFRVSTSWPPANNATLAAHPEWIMVEQADMNAGPQVIGSD
                     YTLDPGSPDVQEYLMSIVRELVTNYELDGIHWDRIRYEQTDAGYPAYTSYARSGLARF
                     KEIAGYAGTPAPTGVPAWNDFRRRSITELVRRAQVEIESVTGNPRQPLRHSAALITWG
                     DAPSNFASTNAYLLFQNWEEWQRLGYLDMAIPMTYYDEDSYPTWYRNWVNREIAWSYD
                     RHMVVGPALYLNEFDDSVAQMLYARNAGADGVCTYAYALTRSGANYDWTWYPYAAANI
                     FTSAAGPPGMPWRSPYTATEGRVWGRVTDSFTGEPIDDAAVQVGALGTVRTDGNGYYV
                     MTLVPASSSGTHYDVTVSRDDYTSQVLTVQVSAGQPVRQDFALDLAPIQTFIVESRAG
                     GQNFDRFSQAGTWANSASKSSAAGTTAGIGSLYTYLGYADRIVTYSFTPTTTASYDVY
                     VTWVTSANACSSAKHTVTHAGGTSTVYKNQLTGGDTWVLLGRYNFNAWTTYGVSQYSS
                     GSSGGTIIRADAVKWQIVGPQPPTITLSPTTLSPSCAEGSNAASQSFMVQNTGGSTLE
                     YFISVNQTWLSVSPASGSSTGEADTITVNYSTSGLSAGTYNATITVSDPNATNNPQSI
                     AVTLTVSPAITVFIVESRSGGQNYDRYSETGAWSNSTAKSTASGCTAGIGSRYCSISS
                     TAKTAVFKFTPTATGSYKIYTTNASTTNSGNPLVHKVTHAGGTTNVNVCQNSTCNPNP
                     CNQWRLLGTFTLNAGTEYKVTLDGSTGAGSAPTGNAGRADAIKWEKQ"
     gene            12337..14982
                     /locus_tag="P5316_05670"
     CDS             12337..14982
                     /locus_tag="P5316_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HRR84481.1"
                     /translation="MDATLCIQRPSPDSGMAGVVLCGGKGSRMGRARIHKVCEPIAGR
                     PAVVRLLDTLQADGVDPIVVVVGHNSGAVVQTIGASHAGVVFVYQRDRLGTGHAARIG
                     VEALARLGYQGTVLVTMGDKWLAPNLVQEAGRRFRRLRADLLLVTTPKRPEGSGGRLV
                     NLPGRGIIGIVERRDIDRARVLRDWFELAASCDTLSRALLRHVGLKRIRPAVKLWRAL
                     GPLAGFSRGSGRVRSSELLQALREAGMDVRVGGTTLGPEKIEQYSKTVNESVYVGDIN
                     VLQAALHRVGRANAQDEYYVTDMIEIIGRGLHCPENDCPGRVVEYEVRRDQVMAFNTR
                     EELFSIEEHVRKQERDLGSERALKKLAPALRSCEEWLAILRPAGRRAQRLLERAYGVD
                     GSVVMRRIGPLRGVVRLWARAFDGKRKIFLVRAPGRLNLMGRHIDHQGGFVHSMALDC
                     EVVMAVSPRQDDVVRLVNADPVAFPARELVIGDWLAALRTPDWLSFVDGQRVRTHLMS
                     TAGDWSNYVLASVLYQQYMHRGRRLCGMDVAVSGNVPIASGLSSSSAMVVAAMEAIVA
                     VNGIAMSPSEIVPACGQAEWFVGSRGGAGDHAAIRLGKAGHVARMGFHPFSLSRYVRL
                     PSDLAVLVAYSGQHAVKSAGARDRFNERVACYRIGMMLLQKKYPRLAKRLQYVRDLTP
                     AYGVFKPHEVDAVLESLPERISRRQIRARLGQEFEERLARVFASHTDPGQYTIRDVVV
                     FGVSECERSRLAADLLDRKDLPGFGEMVLLSHDGDRVSGSPAAEAATAAGRKAESLWR
                     MTGAYACSTPNIDRLVDLASAVPGVYGAQLAGAGLGGCVIILARQSAVPQVRATLARE
                     YYGSLGLAPCIWRVRPVAGGGIIRP"
     gene            15013..15684
                     /locus_tag="P5316_05675"
     CDS             15013..15684
                     /locus_tag="P5316_05675"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRR84482.1"
                     /translation="MSETYRRDSWECPICLKQRRRPPDHKRLYNVLVCRKCRNGFANR
                     RQAAFLVDCVVFYLLVGGAAMLIGLLEDRSPESLESLWAVVWRVLAVIIVPVAFALKD
                     SVNGRSPGKILFGLQVVDAYSRDPIGPGQSFKRNLILAVPYLGLLGLIVGCITMLRGR
                     RWGEGWAGTEVVWLKHATREPFAPAGRYCRVCGYDLTGNISGRCPECGTEIRDRELVP
                     QPRRA"
     gene            15925..17631
                     /locus_tag="P5316_05680"
     CDS             15925..17631
                     /locus_tag="P5316_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRR84483.1"
                     /translation="MARQKKKLGEILVDWKIISPKALEDALKYAEEHRKRIGQALVEL
                     ELCREEDVAKALAAQFGVDYVDVDQTAAIAANMHLIPTKIMKDHQILPLGQENGRLRV
                     IVSDPLDLELLDLLRFTVNPNIDLVIAAPSKIKRFIDKHLSEVGEKMKSTLTSIDEDR
                     EEEEAEGIKIAGEEPDAPIIKLLNQYITEAVNMRASDIHIEPMADRVRVRYRVDGECL
                     ERQKIPKAVQAAVIARLKLMSGIDIAERRLPQDGRIKLTIGGKDIDFRVSTCPAYHGE
                     SCVLRILRPESVRVGIPSLGLEQDDYEQFMKTIKRPNGIFLVTGPTGSGKTTTLYAAL
                     QELNRPDRKIITAEDPVEYNFSGINQCQVRDDIGLTFAKILRAMLRQAPNIILVGEIR
                     DLEVGEVAIQAALTGHLVFSTLHTNDASSAITRLIDMGIKPFLVASSIQAILAQRLTR
                     VICEECKEDDPDPDPKLMRLLRFTEDELRGRLKRGAGCKRCNGTGFRGRLGIFEFLTM
                     TNELRELAFNRASTSQIRKAARASGMRGLLEDGKIKVLRGVTTLGEVAKHAQIEGVVT
                     ED"
     gene            17659..18822
                     /locus_tag="P5316_05685"
     CDS             17659..18822
                     /locus_tag="P5316_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRR84484.1"
                     /translation="MGTLHIDRILETCIRRDGSDIHLTVGRPPVIRVHGHLRSLETKV
                     LEPDDTVALMKAITPDRNQQELQEEGGTDFGFAFGNQGRFRVSVFRQKGHVTLVLRLI
                     PYKLLSFKEIGLPSICQALCRRPRGLFLVTGPTGSGKTTTLATMINYINENFDRHIVT
                     VEEPIEYYHTHKKSLINQREVGLDVPSFAEALRRVLRQDPDVILVGELRDLETMEAAL
                     RAAETGHLVFSTVHTTGCQGTITRIIDQFPHEQQEQIRVQISTNLIAVLSQALCARIP
                     KGRVAAYEFMVVTPAIANLIRENKTFRIDSSIQTGKKFGMQLLDEHLLQLYQDHIISA
                     EEAIDHARNPGEMQDKIEAIQKGLPPEAGPGGGPGGKPKGPDGEPDLPPMPKG"
     gene            18940..20811
                     /locus_tag="P5316_05690"
     CDS             18940..20811
                     /locus_tag="P5316_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRR84485.1"
                     /translation="MAEPLKKTQETPDRKPATAIGAGMEKPPTKPPAPPADRPRGPGS
                     RMPPISQLRGRTLGRILIKMGKLTRTQVGEALNIQKKNPGPIGQILVELGYVDESDVQ
                     IALAAQVGMEPIDLNKIDVPKEVLALVPAKVAHTYKIIPIDYEPSTKTLSIAMDNPAN
                     FQATDDLKTLMGLAIKPYLSNPDDIAASLNKYYPEDQAESISDLINELADDEDLAKFA
                     DRGDGIDLEELKEVAESNPVKKLLNLVLLQAIRDKASDIHFEPFEDEYKIRYRIDGVL
                     YEMVPPPRHIAVAIASRIKVMANLNIAERRLPQDGRIELTVGGNPVDLRVSVLPTMFG
                     ESVVMRVLDRSNVKLSLSRIGLRDDDMNIFRQLIAKPNGIIINTGPTGSGKTTTLYAA
                     LAELNDIETKILTAEDPVEYDIDGLCQCQVNPDIGLTFHRCLRSFLRQDPDVILVGEI
                     RDLETAKIAVEASLTGHLVFTTLHTNDAPSAVARLMDLGLEPFLLTATLEAVVAQRLV
                     RRICENCKAEFHPTEEMLMELDLRPEDVRGKVFMYGRGCDYCNNTGYRGRMGLFEMML
                     MNDDLRDLVMKHASTNILRNAARKMGMRTLRESGLLAIYEGITTIEEVVKETIMEEM"
     gene            20899..22185
                     /locus_tag="P5316_05695"
     CDS             20899..22185
                     /locus_tag="P5316_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRR84486.1"
                     /translation="MPTFQYEAMNQAGQEVKDEIEAQSVEDALAKIRQQGYYPTKLRQ
                     KGGKRGQKAAEAGGAGAKGKKKTVGGFGKVKSKEIVQFTRQLSTLQDAGLPILRCLRI
                     LEQQQKPGKLRLILRNVADDVEGGSTLSEAMAAQPKAFDRLYCNMVAAGEAGGVLDVI
                     LQRLADFMEKAQRLKRKVMGAMIYPIAVITFAIGIVSGLMIAVIPKFKTIFADFGTSL
                     PAPTVALISISEWFVHGTPPGWVIVFLSPVGLMLLVKLIKQSESGRFAIDTIKLKIPI
                     LGNILGKSSVARFTRTLGTLLAAGVPILEAINITRDTSGNEVYARALKSVHDDIREGE
                     SFANPLRAAKICDNIVINMIDVGEETGDLDKMLMKIADNYDEEVETLVDGLVSLLEPV
                     IVIVLGAIVGFIVIALFLPLVELINSVSSGGQKEGV"
     gene            22465..23469
                     /locus_tag="P5316_05700"
     CDS             22465..23469
                     /locus_tag="P5316_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR84487.1"
                     /translation="MTCRVCVCRRRGFSLVELLVVVGIIALLVAILVPSLNVASRRAK
                     SAATLNTIRVLETGLETFKADVVVGGAYPESAVEVPLGRGSITAANPHPSGLTGKVPF
                     NGASYLVWALAGADLLGTPGFLDLTGNGSWRDDTGYSGTHALYQIDPNSRRPIHPRYG
                     PFVAVESMRLARWNDKEKCFFVEKDISRTPLPSTCFLDAFDQPILYYRANPAGTLIAG
                     VQTALSPGAAGLGIYDLYDNFTFTGVVNPVDNQFGPRLNRPQGLDFGAGRIHPLGQLG
                     NPLNPGATLRTFSYMIWDSNVLALPRPQRPDTFILISAGPDHLYGTPDDIGNIQIKKK
                     "
     gene            23633..24478
                     /gene="larE"
                     /locus_tag="P5316_05705"
     CDS             23633..24478
                     /gene="larE"
                     /locus_tag="P5316_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRR84488.1"
                     /translation="MSDAGLQAKLERMKEVLRGLERVAVGFSAGVDSTFLLKVAVDTL
                     GPANVVAVTADSESLTRRELDEARKLAAQMGVEHVVVRTDELDDPNYRANPANRCYYC
                     KQALFRRLDEFIAGRGAMVMVIGVNTDDFADWRPGLRASKEKGVRMPAAEAGMSKADV
                     RELSRQMGLPTHDKPSSPCLASRLPYGEEITPEKLSQIERSEAFLHSLGFHVCRVRHH
                     GNMARIEVPADQIERLCEPATRAKVDATLREFGYQYVAVDIRGFRSGSLNEMLSPSQT
                     GVQAK"
     gene            complement(24822..25913)
                     /locus_tag="P5316_05710"
     CDS             complement(24822..25913)
                     /locus_tag="P5316_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR84489.1"
                     /translation="MPDTGHMSVLGSVSFGWLHAHGGFLFDEGFFFDPRRRLEQERRM
                     QAFVAQRFPDLPIYSIEAHLVQVEGRRRPVALVGGLQPNLILGAAVGARFIIAPDKDP
                     DISPEPLAGLTDVGPLWNIDWPDTWPVSLLLGQVHEMRQTLGPDVAVIPPFFWDTTGR
                     ATTHGIITTAQKLIGERVFLEMADNPGFVRELFAWIVDAYVKLIQLFADAAGITVTGL
                     HTGECSGCMMGPDQFAEFALPCLNNLADRVGPLRLHSCGNSNHLLDVFREVRNVAILN
                     LGSGTSVAAVRDRFGPIRIDIMPQTHLLTAGSPQDMDAWVRQCVADNGDARLQFEYHL
                     DLNQPEQNCLQIHRTLEEMGIHSPRLQVY"
     gene            complement(25984..26262)
                     /locus_tag="P5316_05715"
     CDS             complement(25984..26262)
                     /locus_tag="P5316_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRR84490.1"
                     /translation="MFPRTTEATPIRQYRLRLVFTDGTQGEANLQDWVVGQGGVFAPL
                     EDLGYFRQVTVNTELGTIQWPNGADLCPDVLYSLVTGRPIPAPESATP"
     gene            complement(26374..27393)
                     /locus_tag="P5316_05720"
     CDS             complement(26374..27393)
                     /locus_tag="P5316_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84491.1"
                     /translation="MPKKTWNVAVVGPGWVAGAYAKSFCKRDDVRIGYVVGRTYEGAA
                     AFTREHNLNCPCSDDLCAALKDPSIDIVGVFTPHHLHAPITLAAAKAGKHIIIEKPVC
                     LTLEDMKAIRAAVREAGVKTIIGFVLRWNPLLKIIRRNIAEGRLGRIIFAETDYLHGI
                     VGKPYTKPWHTTRATAGTSLLIGGCHAIDAIRFLVGRPVVEVSAYSAGRTNALDYPST
                     ELALLKFDDGSIGKVGCCLEAKLPYVFNVEVYGTAGSFRNNQFAGDLYDGQTGFATIP
                     TILPDSGDVSHHPFDGEVAELIDALNAGRRPMPDIEDAAETMEICFAAEISARQRQAV
                     KLPLG"
BASE COUNT         5263 a         7709 c         9187 g         5311 t
ORIGIN      
        1 gccgcaaccc aagcgttctg cgatccggaa acatgcacgc aaaatgcgca ccagttgcga
       61 cggaagggac tatagacgtc ggctagcgga agaaactgta tcggaagatc gcgatgactg
      121 ctttgatgcc gtccacccaa gtaatctttt tcccctctga gtagtcccgg ccggcgtagc
      181 tgatgcccac ctcgtaaacg cggcagccgg tcttggcaat cttggcggtg aactcgggct
      241 cgaactcgaa gcgatcgctc ttgagctcga aattgcgcag gatctcgcgg cgaaagacct
      301 tgtaacagac ttccatatcc gtgaggttca agttggtaag catgttggac agcagggtca
      361 ggatcttgtt tccgagcatg tgccagaaga aaaggaccct gtgcgcctcg ccgcctacga
      421 agcgggagcc gaaaacgacg tcggcgcggt tgtcgagaat gggggcaaga agtctcgggt
      481 aatcgcgagg gtcgtattcg aggtccgcgt cctgcacgag gatgatgtca ccggtggcct
      541 tgtcgaagcc cgttcggagc gcagccccct tgcctcggtt gtgagggtgc atgagcacct
      601 ggatgtgttg gccggcatgc tcgcggcgga tgcggtcgta aagatcacgg gtgccgtcgg
      661 tggaggcgtc atcgacgagg atgagttcct tttccatgcc gacgtcaacg gcgagcaccc
      721 ggcgaaccaa ttcctcaagg gtccggactt cgttgtagac aggcacgacg atgctgagtt
      781 tcacgcgtcg cctccattac ctgcgtgacc ggtgcccatc ttagaagcga atacgtggag
      841 cgggcaagtc ggtgagaggc cgcgttgggg gactcgggtg aaatgactta tcgtcccatg
      901 cgtttcgcag aggtttgcgg tgatatccgt ggggacatgt tgtgaactcg tggggtgggc
      961 ggccggctgg ttggatgagt gccagggggg ctgtccaagg cgggtccctg aggtcggcga
     1021 taagtatatt tatgacaaga ggttgaagtc cgtgagacat ctgttgcaaa gatggttgca
     1081 ctgtcgagag cggggtgggg gcacgggcgg tcaattgccg ggcttttgcg tatagggccg
     1141 ttggcctaga attaccgatt cgagattcta cagtacataa gttcttgttc cgatcggctt
     1201 caaggtgacg caccatgcgg agcgggtcgg gcggttacag cgagtgttca gcatgcgtta
     1261 aacggaggtc gtcgtgacga aaggccatat gtccaaggaa ttgatcattc tggcggcgtc
     1321 attggtattc acaaccttgc caggtgccgg cgaggtgaag ggtcaggtcc ctcccggcgt
     1381 tgctccggtt ccgccggcgt cgtcggttgc cccggccgat ccgaatgggc ccaagctgcg
     1441 atgcgacgag aacacgttcg atttcggcga tgcctggtcc ggcacggtgg tcgagcacgc
     1501 gtacgtgatt cacaacgacg gcaagtcgcc gctggagttg ttgaatgtca agacgacttg
     1561 cggttgcacg gttgtcgatt atgacaaggt gatcgccccg ggggggcagg gcaaggtgac
     1621 ggtcaagctg accctgagca aagcggttca gtctcagccc gtatcgaggt ccgttcaggt
     1681 tgagagcaac gatccggcca acaaggtcgt tcccttgacc atcaagggca aggtgaagcc
     1741 tcgtgttgcc ctggaaccgt ccaatgccgt tttcggcacg gtgaccgacg agacggacgt
     1801 gacgcgggtg atcaaggtca cgaattacac cgaagggcac atgaaactgg agcccgttcc
     1861 gttgcctccg ggtcagcaat ccgtcttcaa ggcggaagtc accgaggtgg agccgggcaa
     1921 ggtggcggag atcaagatca caggcgtgcg gccgtttgcc gagcggaaca attccacagt
     1981 gttgcagttt accaccggtc tggataagga cgaacggatc agcgtgccgt gttcgttata
     2041 ctcgccaccg ttgctggagg tgagtccaaa ccggcttccg ttgttcctgc cgctgacccg
     2101 ggagttcatt cgtcaggtga cgatcaacta cagcggcaag gaggatttca agatcgagtc
     2161 cgtggtgccg ggcaatgatc agatcaagac tgagctgaca cctgttgcgg ggtcgaagtc
     2221 tttccggatg gtggtgcgcg tgccccagga ttttactccg ccgaaccatg accccatcga
     2281 tatcatcctg aagaccaacc tcaaggagaa gtcggagttg caggtaacgc tggagcctcg
     2341 cgtgtctccg acggcgccgc aaccgcagcc cccgccggtg gtggtggctc gggtggagga
     2401 tctcctgggc aagccgacgc cggtgaccag tcagatgccg ttgaaggctg ggcaggccgt
     2461 tcagttcggg accggcaacc agccggccgt ggtgaatttc tgggcggctt ggtcgtcgca
     2521 gtgccgccgg caactgccgt acatgaggac tctgcagagt ctgtacgggg tcaagggcgt
     2581 caccttcttg aacatcagcc tggactcgct caagccggtc ggcgaagttc tggaagcggc
     2641 gaaggaggca gggctgacgg aagcagcgct ggcggcggat ccgcaataca cgactgccgc
     2701 acggttcggg atcaagaggg tgccgacggt cttattgatc ggcaggaacg ggacggtgga
     2761 ggctgttcac gaaggcgttc ccgcggcgga acagatggct gagtacgagg agatgctgag
     2821 gaacgagatc gacgctttgc tcgagggcaa gggtcgcagc gagttcggca gcataccccg
     2881 tccggccggc agcgctgagc tgctcgaacc ccagggagcc ggcactaccc cgctggggcg
     2941 gatgccgcgg ttgacggtgg agtcggcccg acaagacctt ggtggggtca agccggcgag
     3001 caaggtgacg gccaagctct actatcgtaa tgacggcatg gagccgctca cgatcaactc
     3061 agtgactgca agcgaggggc tctcggtcgg atccgagtac acgaagagcc tgacaccggg
     3121 ggccatggcg gttctcaact gtacatttga gagcggcgca cggccgggac ctttcgagca
     3181 caaagtgacg ctgacgacga acgatccgac gcgaccgaag ctggacgttg tgttgttggg
     3241 taccgttcgg ccttacctgg aatgcgatcc ggcgacgggc atcgactttg ggcgtcaccc
     3301 ccgaaagcac acgatggggc gactggcgac cattctgtac cacgggacgg acgagatcga
     3361 gtacctgtcg gctgaatgca cctcgccgaa attcgaggtc atcgttgaga agcttcgcgg
     3421 cagtcaaaac gccaagctgg tggtcaaccc caagccgccg ttcgaccttg gcgaactgaa
     3481 ggcgaccatc aaggtgacga cgacctgcaa ggagcagccc acgctcgagg ttccgttgag
     3541 gctgtacaat cccaagcgga tcgaggtgac accggaagcg atcgttatcg gcgggccggc
     3601 ccgcgctcag cgattcagcg tgacgatcgc caacaacggt ctggacaacc tgagtatttt
     3661 cggcatcacg cggagcaaca atctcatccg ggcgaggatc tatccggacg ccgacgacgt
     3721 ttcctacaag ctcgatgtgt tcgttccgcc gacctacgtt ccgccggccg aaggcgacaa
     3781 gatcaccatc cggaccgacg acaaggagtt tggcgagatc gtgatcccga tcaagccggc
     3841 ggcaggcgga tgaaatgctc cacgcgttgt cgggatgctc tttcttgttt tggcggagca
     3901 tccagggcgt gctgccttct tgcgggccgc aagaccgagg cgtgcggtga ttcgggggcg
     3961 cttgggcaag gcgcgcgggt ttccggtcgc agcggttcag ccaacggttt tgcccgggtc
     4021 gctctccaca atgggcacgg tcttggatgg ggttgcctcc aagcactcag cgaggttctc
     4081 gcgtgcggtg ccgatcggcc ggatgccgaa tttctcgctc aggacggcga agacattcgt
     4141 tgacacgaac gccggcagcg acggtcccag tcggatgttc cgcacgccca ggtgcagcag
     4201 cgtcagcaga atgcacactg ccttctgctc gtaccaactc aggatcagcg acagcggcag
     4261 atcgttgact ccacacttga gggcgtcggc caagccgata gcgatcttga tggccgagta
     4321 ggcgtcgttg cactggccga cgtccagcaa tcgcggcagc ccctcgacgg tgccgaagtc
     4381 gagcttgttg aatcggtact tgccgcaggc cagcgtcagg atcacgcagt cgtcgggcac
     4441 ggcctcagcg aagtcggtgt agtaattgcg gccttccttg tggccgtcac aaccgccgat
     4501 caggaagaag tgacggattc tgcctgcctt gacggctttg atcaccttgt cggccgcacc
     4561 aagcaccgag ttatggccaa atcccaccag gatcgtcttt tccggctcgg tgtgtctgaa
     4621 gccgtcggcg gcgagggcgg cttcgatcac cgggcggaag tcgcggtcgg tgatgtgggt
     4681 gacacccggc catgcgacca ggccgcaggt gaaaagccgg tgacgatagc tgtctcgggg
     4741 cttctgaatg cagttggtag tcatgaggat cgccccgggg aagcgttcga attcgtcctg
     4801 ttgctggaac caggctccgc cgtagttgcc gaccaggtgc ttgtacttct tgagctccgg
     4861 ataaccgtgg gccgggagca tctcgctgtg cgtgtagatg ttgatgcctg tcccctcggt
     4921 ttgcttgagc agttcttcca gatccttgag atcatggccc gagatcaaga tcgccttgcc
     4981 cttgaccggc gtgatgcgca ccggtgtcgg caccggatgg ccgtaggcac tggtattggc
     5041 cctgtccaac agctccatga ccttcagatt aacctcgccg cacttgagta ccagggacag
     5101 caggcttgcg gcggtgtggt ccgttcgtgt caggctgtcg agggcctcat ggaagaaggc
     5161 gtaaacgccg tcgtcctcgt gtccgagaat catcgcgtgg tgggcgtagg ccgccgtgcc
     5221 cttgaggcca taggtcagaa gttcttcgag gccggccaca tccatcccgc ggacgcgaat
     5281 gcggtccatg atgccgacgt cctgaccctg tgcgacgaga ccgtcaatgt cgccggcggg
     5341 ggaccaaatc gccggaccgg ttagtatttc aggctgcttg ccggccttga tgcaggcttg
     5401 ctcgtagagt atgcgggctc tgtccctgat cgaggcggcc ttgtgcagtt cctcgcccat
     5461 ccgctccggg ctgaagttca cattggtgac ggtggtgaac agggcttcga cgatgaagcg
     5521 gtcaacctgt cggtcgcgag ctcccaggta gccggcgcga tgcgcgtgca tcgagatgcc
     5581 cttggctgca tggatcagca ggtcttgaag ggccgcgacg tcgggggtct tcccgcaaac
     5641 gccgatggta ctgcaccctg tccctttggc cgcctgttca cactgaaaac agaacatcag
     5701 gaaacctcca tttgggccga cggcaggcca tcgtgacaca tctccgagag ctgccccggc
     5761 gggttttcga atcgagagag tatgggcagg ttggctcgga gcagcccttg atctgtgtca
     5821 aagtgatgcg gttttcgtag cggggtcggc aatccgtatg ttagtcattg ctatacaaga
     5881 ggttatgatc cggccgcggg tcatgtcgca tttgagcagc tttgcgtcgg tggcgccaat
     5941 tccggaaggc aacagccatt gaggatggag gctcagatga gggcgtggcg aaccttgtcg
     6001 acggaattgg cacgtgatcg acgtcgaatt ttggagaaaa cggttgacag acgtttgcgt
     6061 cacggtattg tgtcgtgcgg agcaggcggt ctgattgtgg cggatgcggt atgtggcacc
     6121 gtatccggcg tctggtagga aaagctggtc atctcaggct ctgtcatcaa tgagcagccg
     6181 ctgcccaaaa cgctgatgcg aaaggccggc tctgtgccgg tttcacattc cggttgtctc
     6241 tgtacgagga ggatgatcat gtcgaggaag ctgcattctc aggcagcgat gtgtgttgtt
     6301 gtgtttttgc taccggtggc ggtgatgggc gaggagctgc cgccggagga ttcctacgat
     6361 ccgggggcgg ggtattactc ggccgggtat caaccgctca tgccggtatt cgaggaggcg
     6421 gccgcggagt acgacgtgcc gcttgacctc ttgctggctc tgggcaaggt tggcagcgcg
     6481 tttgaagatc gcgggaccga gccgacgatc gagggcggct acgggatcat ggctctgcgc
     6541 gatagcgaat actctgattc gttgaagcaa gcggcgagac tgctgaagct ggacagcgag
     6601 gttctcaaga tcgacgcagc cgcgaacatt cgcggggcgg cggcagtgct ggaccgggcg
     6661 gccaagaagg ccaagatcga ccgcaaccaa ggtttacgtg cctggttgcc ggtggtcatc
     6721 gaatacgccg gcctcgatgc cgaaagcagc aagttctttg cccggggcat ttatcagaga
     6781 ttgcagaacg gtttttcgaa ggtgaattcg gcgggcgagg ttttcgaggt tctttcgcag
     6841 tcggttggcg ttgatctcga cagtctggtg ccgaaggggt tggtggcggt ggaggcgacg
     6901 actttcgact atcagatcat gagtgcgccg ggcgattgcg gcaacccgga ctacgctttg
     6961 gccacttggg atccggctcc gacctgcaac tactcgacgt cggtgaccaa caaggacacg
     7021 attgtgattc acgttgcgga gggctcggcc gcggggacca tctcctggtt caagaactgc
     7081 agttcaggag tggccgccca ctacgtcatg gatgaagccg gagcgatctg gcaaatggtt
     7141 tgcgagcgac acgtcggata ccacatcgtc tgctacaaca gcagggccat cggtattgaa
     7201 cacgagggtt ataccgccga ccccagtcat ccggaggcga tgtatcaggc ctcggcgaac
     7261 tgggcccggg acgtctgcaa tcgctggggc attccgaagc agaaagcccg ttcggcgccg
     7321 ggcatcgtgg ggcacgcgga cgtgagcgag tgtgttcggt gtacggacca catggacccg
     7381 ggccatggtt gggactgggg tcattacatg gcgctggtca accaggcacc ggatccgatt
     7441 acgttcattg ttgagagccg cgcagggggc aagaacaatg ccaactactc ggaagtgggc
     7501 tcgttcggga actccagcgg caagagcacg gtgtgggact gcaccagcgg catcgggagc
     7561 cggtacgctt acatgaacgg cactaacagg tgcagttaca gtttcacgcc cacgacgagc
     7621 gggacctacc gggtttatgt cacctggtgc acctcggcca acgccactca gcaattggag
     7681 cacattgtca cgcacgccgg cggttcgacg agcatcgtgc ttgacacgga cgtggatacg
     7741 aatccctgcg ggcgcaacaa ttggaacctg ctgggggaat tcgtgctcaa ttccggcgtg
     7801 cagtacagcg tgacgcagac cagccaaaac tatcatgacg ggatggtatt gcgggccgac
     7861 gcggtcaagt gggagtatgt tggcggcggc gggggcgtcc agcctccgac gatcactcag
     7921 caccccgcgg cccagagtgt ctgtccgggc tcgacggcgg ttttcagcgt gacggccacg
     7981 ggcgagggca cactgaccta ccagtggcaa aagaaccagg tgaacctgac gaacggcggg
     8041 cattactccg gagtgaccac cgaggcgctg acagtgtcaa acgcggacgg caatgacgcg
     8101 gcgaattacc ggtgtgtggt gagcaacgcc ggcggcagta cgacctcaaa cgcggcgggc
     8161 ttgacgctca aggccgcgac tacgatcacc caacatccat cggatcagac ggtggcgacg
     8221 ggcgggacgg ccaacttcag cgtcgccgct gccggggacg gcgccctgac ctaccagtgg
     8281 caaaagaacc aggtgaacct gtcgaacggc ggccactact ccggcgtgac gaccgcgacg
     8341 ctgaccgttt ccaatgccga cagcaacgat gcggcgagct accgctgcgt ggtgacggcc
     8401 ggctgcggga tcgcgacctc gaactcggcc acactgacgg tgaccacggg gggcaccacg
     8461 atcttcttcg acaatttcga tagttatgcg aaccagacgg cgtttaccgc cgtgtggccg
     8521 atttcggtat cgcccggcgg gaccctgtcg acggcgctgt atttcagctc gccgaagtcg
     8581 atctacatcg gcacgtcggc ggcccgaaac cagcgcaact ttactgaaac gacggcctcg
     8641 gacgccgttc cgatcgtgtg gaagtttcgc ctgtacgata acaacacctc caatctggat
     8701 cgtcagtggt gcgagctgct ggatgcgtcg cccagcgtcg ttcagttggt agcactgggc
     8761 aagtccaacg tgcaggaggc gatgcggacg tactacgcag ctcgcgtggc ctattcgccc
     8821 ggaccgggtt gggtagcgct gaacggcccg ggtgctccga ctcggagcgt gggatggcac
     8881 gaactgaaag ccgtgatcaa gagcacgacc attgacttct acgtggacgg cgtgttggcc
     8941 aagtcgggcg tatcctacgc caacagtcaa ggccagttca gcttcgaccg tgcgagactt
     9001 ggttcgggtt acagttcgac aagtctggcg tattacgacg attactcggt cgtgagcggt
     9061 caataagcag gtcagggtgc gcggcacggg ccttgggggg gccgtgccgc ctccggtttg
     9121 tcgtatgggt cattctgcaa atctcggcca taaagccatt tctcgaggag gatcaatcca
     9181 tgtcatttgg actcgtctct cgtccggtca ggattgccgt gatcctggcg gctttgccgg
     9241 tcggccgtgc cgcagcccaa accacggagt tccggggcat gtgggcggat gtctggcatg
     9301 aaggatacaa gagcgcggcg cagatcgacg cgatggtggc tcgcgcggtt caaggaggat
     9361 acaacgcgat ctttcccgag ctgctggctt accaggatag tggatcgagc gcccacgggg
     9421 cgtattggaa ctcggccatt gtgccgaagg caactgatat tcaaggaggc atcgacccgc
     9481 tggcctattt gatacagcag gctcatgcgc agggcatcga ggtacatgcg tggttgaccg
     9541 cgtttcgggt gtcgacgagc tggccgccgg cgaacaacgc gacgctggcc gctcaccccg
     9601 aatggatcat ggtggagcag gcggacatga acgccggtcc gcaggtcatc ggctccgact
     9661 acactctgga tcccggttcg ccggacgttc aggagtattt gatgagcatt gttcgcgaac
     9721 tggtgaccaa ttacgaactg gacggcattc actgggaccg gatacgctat gagcagacgg
     9781 atgccggcta tccggcttac acgtcctacg ccaggtccgg cctggcccgt ttcaaggaga
     9841 tcgccggata cgcgggcacg ccggcgccca ccggcgttcc ggcatggaat gacttccggc
     9901 ggcgaagcat tacggagctg gtgcgacgcg cccaggtgga gatcgaatcg gtcaccggca
     9961 acccgcggca gccgctgcgt cacagtgccg cactgatcac atggggcgac gcaccgagca
    10021 acttcgccag cacaaatgcc tacctgctct tccagaactg ggaggagtgg cagcggcttg
    10081 gctatctcga catggcgatc cccatgacgt actacgatga ggacagttat ccaacctggt
    10141 atcggaactg ggtgaaccgg gagattgcct ggagctatga tcggcacatg gtggtcggcc
    10201 cggctttgta cctcaatgag tttgacgaca gtgtggcgca gatgctctat gcgcggaatg
    10261 ccggcgccga cggcgtgtgc acctatgcgt acgctctcac gcgatcgggc gcgaattatg
    10321 actggacgtg gtatccatac gcggcggcca acatattcac ctcggcggcc ggcccgcccg
    10381 gaatgccttg gcgcagtccg tacactgcaa ccgaggggcg tgtctgggga cgggtgacgg
    10441 actcgttcac cggcgaaccg atcgatgacg cggccgtgca ggtaggtgcg ctgggcacgg
    10501 tgcggacgga cggtaatgga tactatgtca tgacgctggt accggcgagc agttccggca
    10561 cccactatga cgtcactgtt tcccgggatg actacacatc gcaagtgctg accgtgcagg
    10621 tgtcggccgg tcagcctgtc cgccaggatt tcgccctgga tctcgcgccc atccagacgt
    10681 tcatcgtaga gagtcgcgcg ggcggccaga acttcgaccg attcagccag gcgggcactt
    10741 gggcgaacag cgcgtccaag agcagcgcag cgggtaccac ggccggcatc ggcagccttt
    10801 acacgtacct ggggtacgcc gatcgtattg taacgtacag cttcacgccc acgacgaccg
    10861 cttcgtatga cgtgtacgtg acctgggtaa cctcagccaa tgcatgcagc agcgccaagc
    10921 acaccgtcac ccacgccggt ggaaccagca cggtctataa gaaccagttg accggcggcg
    10981 acacatgggt gctactcggc cgctacaact tcaacgcgtg gacgacgtac ggcgtttctc
    11041 aatactcatc ggggtcctcc ggcggcacga tcattcgggc ggacgcggtc aagtggcaga
    11101 tcgtcggacc gcagccgccg acgatcacgc tttccccgac gacgctgtcg ccgtcgtgcg
    11161 cggagggcag caatgcggcg agtcagagct tcatggttca gaacaccggc ggctctaccc
    11221 tggagtactt catcagcgtg aatcagacct ggctcagcgt gagcccagcc agcggttcgt
    11281 ccaccggcga agctgatacg atcacggtga attacagcac gtcgggcttg tcggcgggca
    11341 cctacaacgc gacgatcacg gtcagcgatc cgaatgcgac gaacaatccg caaagcatcg
    11401 ccgtaacatt gaccgtcagt cctgcgatca ccgttttcat tgtggagagc cggtcaggcg
    11461 ggcagaacta cgacaggtac tcagagaccg gggcatggtc gaacagcaca gccaagagca
    11521 cggcctcggg ttgcacggcc gggatcggca gccgttactg ttcgatcagc agcacggcca
    11581 agacggcggt attcaagttc actccgacag ccacgggttc gtataagatc tacaccacca
    11641 acgccagcac caccaacagc ggcaacccgc tggttcacaa ggtgacgcat gccggtggaa
    11701 cgaccaacgt gaacgtctgc cagaactcaa cctgtaaccc gaatccctgc aaccagtggc
    11761 ggctcctggg gactttcacg ctgaacgccg gaacggagta caaagtgacc ctggacgggt
    11821 cgaccggggc aggttccgct ccgaccggta acgccgggcg agcggacgcc atcaagtggg
    11881 agaaacagtg accgtcagag cggcggtcgg agggtatgca aagaacgggc cttcggtgag
    11941 aggtggcacg gtcggtagcg gccgtgccac ttcgtcaggt gccgaccttg ttgcggtcgc
    12001 agttgcgacc ttttcagcag gggcattctg agcagggccg gacgaggatt gtgcaggcgt
    12061 gcgcggccgc acgggcgaag catggtcgca tcttacggtg cgatcctctt cgcggttcgg
    12121 cgatcattga atgaggatgg gcatcgtcgg acccggatgc gaagccgggg ccagcggtgg
    12181 tttgccacct attggaccgg cggggctctt ttcgagaatc cgttcacggg tataagatga
    12241 gcgcgtttga ccggattcct tatggcgagt gcgacggcga ggcgcgtagt ggctttgaga
    12301 gcggacagga atcggagcgg cccggcgaag gctgcggtgg acgcgacatt gtgcattcag
    12361 cggccatcgc cggattcggg catggccggc gtggttctgt gcggcgggaa aggcagccgc
    12421 atgggacggg cgcggatcca caaggtctgc gagccgattg ccggtcggcc ggccgtggtt
    12481 cggttgctcg atacactgca ggccgacggc gttgacccga tcgtggtggt cgtgggacac
    12541 aacagcgggg cggtggtcca gaccattggg gcatcccatg ctggggttgt tttcgtttat
    12601 cagcgagacc ggttgggtac gggccacgcc gcccggatcg gcgtcgaggc tctggcgcgg
    12661 ctcggttacc agggcacggt gctggttacg atgggcgaca aatggctggc gccgaatctc
    12721 gttcaggaag ccggcaggcg ttttcgccgg ttgcgagccg acctgttgct ggtgaccacg
    12781 ccgaagcggc cggaaggcag cggcggacgg ttggtcaatt tgcccggccg gggcattatc
    12841 ggcattgtgg aacggcggga catcgaccgg gcgcgcgttc tgcgggactg gttcgagttg
    12901 gccgcctctt gcgacacact ttccagagct ctgcttcgtc acgttgggct taagcgaatc
    12961 cggccggcgg tcaagctgtg gcgggcgctg gggccgcttg ccggtttctc acggggttcc
    13021 ggcagggtcc gatcgtctga gttgttgcag gcccttcgcg aggccgggat ggacgttcgc
    13081 gtaggcggca cgaccctcgg gccggagaag atcgagcagt atagcaagac ggtcaacgaa
    13141 tccgtttacg tgggtgacat caacgtttta caggcggctc ttcatcgcgt cggacgagcc
    13201 aacgcccagg acgaatacta tgtgaccgac atgatcgaga tcattgggcg cggcttgcat
    13261 tgcccggaga acgactgccc gggcagggtg gtcgagtatg aggtccggcg cgatcaggtg
    13321 atggctttta acactcgcga ggaactcttc agcatagaag aacacgtccg caagcaggag
    13381 cgtgacctgg gtagcgagag ggcccttaag aagctggctc ccgccttgcg gagctgcgag
    13441 gagtggctgg ccattcttcg tccggcgggg cggcgtgctc aaaggcttct cgagcgcgcc
    13501 tatggtgtgg acgggtctgt ggttatgcgc cgcatcggcc ctctgcgggg cgtggtgcga
    13561 ttgtgggcca gggccttcga cggaaagcga aagatttttc tggttcgcgc gccggggcgg
    13621 ctgaatctca tgggccgtca cattgatcac caaggcgggt ttgttcactc gatggcgttg
    13681 gactgcgagg tggtcatggc ggtgagccca cggcaggacg acgtcgttcg gttggtcaat
    13741 gccgacccgg tggcttttcc cgcccgcgaa ctggtcattg gcgattggct ggcggccttg
    13801 cggacgccgg attggctgag tttcgttgac ggccagagag ttcgcacgca cctgatgtcc
    13861 acggccggag attggagcaa ttacgtcctg gcgtccgtgc tctatcagca atacatgcat
    13921 cgtggacgac ggttgtgtgg gatggatgtg gcggtcagcg gcaacgtgcc gatagcctcc
    13981 ggtctttcga gcagttcggc catggtggtg gcggcgatgg aggcgattgt ggccgtcaac
    14041 ggcatcgcca tgagcccgtc ggagattgtg ccggcgtgtg gccaagccga atggtttgtc
    14101 gggtcgcggg gcggggcggg cgatcacgcg gctattcgcc tgggtaaagc cgggcatgtg
    14161 gcccggatgg gctttcaccc cttttcgctt tctcgatacg ttcgtcttcc ttctgatctt
    14221 gccgttctgg tcgcttacag cgggcaacat gcggtgaaga gcgccggggc ccgcgatcgg
    14281 ttcaacgaac gtgtggcctg ctaccggatc ggcatgatgc tcttgcagaa aaagtacccg
    14341 cgtctagcca agaggctgca gtacgtgcgc gatctgacgc cggcctacgg cgttttcaag
    14401 ccgcacgagg tggatgcggt gctcgaatcg ctgcccgagc ggatctcacg gcgtcagatc
    14461 agggcccgac tcggtcagga gttcgaggag cggttggcca gggtctttgc ctcgcatacc
    14521 gatcctggtc agtacaccat tcgcgacgtc gtggttttcg gcgtcagcga gtgcgagcgg
    14581 agccggctgg cggccgactt gctggatcgg aaagacctgc ccggtttcgg tgaaatggtc
    14641 ttgctgtcgc atgatggtga ccgggtcagc gggtcgcccg cggctgaggc ggcaacggca
    14701 gccggacgga aggctgagag cctttggagg atgaccggag cttatgcgtg cagcaccccg
    14761 aacatcgacc gtctggtgga cttggcctct gcggtgcccg gtgtttacgg tgcccagctt
    14821 gccggggcgg gactgggcgg ctgtgtcatt atcctggcgc gccagtcggc cgtgccgcag
    14881 gtccgagcga cacttgcccg cgagtactac ggttcgctcg ggctggcgcc ttgtatctgg
    14941 cgggtccgcc cggtggccgg tggggggatc atacggccgt aggaatcgag aattgagaat
    15001 ggagaatggg gaatgagtga gacctatcga cgcgattcct gggagtgccc gatttgtctg
    15061 aagcaacggc ggcgcccgcc ggatcacaag cggctttaca atgtgctggt gtgcaggaag
    15121 tgccgcaacg gttttgctaa tcgccggcag gcggcgtttc ttgtcgattg cgtggttttc
    15181 tatcttctgg ttggtggggc ggccatgctg atcgggctct tggaagaccg ttctccggag
    15241 agcctggagt cgctgtgggc cgttgtttgg cgggtgctcg ctgtgatcat cgttcccgtc
    15301 gcatttgccc tcaaagactc cgttaatggt cggtctccgg gcaagattct cttcggccta
    15361 caggttgttg acgcgtacag tcgcgacccg atcggtccgg gacagtcgtt caaacgaaac
    15421 cttatccttg cggttcccta cctgggtctc ctgggcttga ttgtcggctg tatcaccatg
    15481 ctgcgagggc gccggtgggg tgaggggtgg gctgggacgg aggttgtctg gttaaagcac
    15541 gccactcgag aaccgtttgc tcccgcgggg cgctactgcc gggtgtgcgg ctacgacctg
    15601 acggggaata tcagcggccg gtgcccggaa tgcgggaccg agatccgcga tcgggaactg
    15661 gtaccccagc cgaggcgagc ttagcggtca gcgaggtgtt tcaggcggtg gggcaactgc
    15721 ccgagccagc ggccacgatt gcggcagcgc cccagtcttg cgttgggaaa tgtgtccagc
    15781 ttgatgttgt agtcggcttg ccgatagaat aggaggggta ggcgagaaca ggccgatcgt
    15841 gggatcctgc gccgcgatct tcgccgccca ccggctgggt tagctgacgt atttccttac
    15901 cagattaaga cttgccgcga actcatggcc cgacagaaga agaaattagg cgagatcctg
    15961 gtggactgga agatcatcag tccgaaggct cttgaggacg ccctcaagta tgccgaggag
    16021 caccgtaagc ggatcggcca ggccctagtt gagctggagc tctgccgtga ggaggacgtg
    16081 gccaaggctc ttgctgccca gttcggcgtg gattatgtcg acgtcgacca gacggcggcc
    16141 atcgctgcga acatgcacct gattccgacg aagatcatga aggatcacca gattctgccc
    16201 ctcggccagg agaacggccg gcttcgcgtc atcgtcagcg atcctttgga tcttgaactg
    16261 ctcgacctgt tgagattcac ggtcaacccg aacatcgacc tcgtcattgc cgccccgagc
    16321 aagatcaagc gcttcatcga caagcacctc tccgaagtcg gcgagaagat gaagagcacc
    16381 ctgacgagca tcgacgagga ccgcgaagag gaggaggccg agggcatcaa gattgccggc
    16441 gaagagcccg acgccccgat catcaagctt ctgaaccagt acatcacgga agcggtcaac
    16501 atgcgggcga gcgacattca catcgagccg atggcggacc gcgttcgtgt ccgataccgc
    16561 gtcgacggcg agtgcctgga acggcagaag attccgaagg cggtgcaggc ggcggtgatc
    16621 gcccgtctca agctgatgtc gggcattgac attgccgagc gccgactgcc ccaggacggt
    16681 cgtatcaagc tgaccatcgg cggcaaggac atcgactttc gcgtgagcac ctgcccggca
    16741 taccacggtg aaagctgcgt gctgcgtatt cttcgtcccg aatcggtccg ggtgggaatt
    16801 ccgtcgcttg gtctggagca ggatgactac gaacagttca tgaagaccat caagcgtccc
    16861 aacggcatct tcctggtgac cgggccgacg ggatcgggca agaccacgac gctgtatgcg
    16921 gcgttgcagg aactcaaccg cccggacagg aagatcatca ccgcggaaga cccggtcgag
    16981 tacaacttca gcggcatcaa ccagtgtcag gtccgcgacg atatcgggtt gactttcgcc
    17041 aagattctcc gggcgatgct gcgtcaggcc ccgaacatca tcctcgtcgg cgagattcgt
    17101 gacctggagg tgggggaggt agcgatccaa gcggccctca cgggccacct ggtgttcagc
    17161 acgctgcaca ccaacgacgc cagcagtgcg atcacgcgtc tgattgacat ggggatcaag
    17221 ccgttcctgg tcgccagttc gatccaggcg atcctggccc agcgtctgac gcgggtgatc
    17281 tgcgaggagt gcaaagagga cgatccggac cccgatccga agctgatgcg tttgctgcgt
    17341 ttcaccgaag acgaactccg gggccggctc aagcggggcg cgggttgtaa gcggtgcaac
    17401 ggtacgggct tccgcgggcg gctgggtata ttcgagtttc tgacgatgac caacgagctg
    17461 cgggaactgg cgtttaaccg ggcttccacg agccagatcc gcaaggcggc ccgggcgtcg
    17521 ggcatgcgtg gtctgctgga ggacggcaag atcaaggttc ttcggggcgt gaccactctt
    17581 ggcgaggtgg ccaagcacgc ccaaatcgaa ggtgtggtga cggaggacta gtagccgggg
    17641 gaagaattgg caggtgttat gggtacgctg catatagatc gtattcttga gacgtgcatt
    17701 cggcgagacg ggtctgacat tcacctgacg gtgggcaggc ctccggtgat tcgggtacac
    17761 gggcaccttc gctcgcttga aacgaaggtg ctcgagccgg atgacacggt ggcgctcatg
    17821 aaggccatta cgccggatcg gaaccagcag gagttgcagg aggaaggcgg caccgacttc
    17881 gggtttgcct tcggcaacca ggggcgattc cgggtgagcg ttttcaggca gaaggggcac
    17941 gtgactctgg ttctgcgtct gattccttac aagctactga gcttcaagga aatcggtttg
    18001 ccctcgatct gccaggccct gtgccgccgg ccgcgaggct tgttcctggt cacggggccg
    18061 acgggatcgg gcaagaccac cacgctggcg acgatgatca actacatcaa cgagaacttc
    18121 gaccggcaca ttgtcaccgt ggaggagccg atcgaatact accacaccca caagaagtcg
    18181 ctgatcaacc agcgtgaggt gggtctggac gtcccgagtt tcgcggaggc cttgcgtcgg
    18241 gtcctgcgtc aggacccgga cgtgattctg gtcggcgagt tgcgcgacct ggaaacgatg
    18301 gaagcggcgc tccgcgcggc cgagacgggt cacctggtgt tctcgacggt acataccacc
    18361 ggttgtcagg gtacgatcac ccgcatcatc gatcagtttc cccacgagca gcaggaacag
    18421 attcgtgtgc agatctcgac caatctgatt gcggtgcttt cccaggctct gtgtgccagg
    18481 attcccaaag gccgggttgc cgcgtatgag ttcatggtgg tcacgccggc catcgccaac
    18541 ctgatccgcg agaacaagac gttccgtatc gattcgagca ttcagacggg caagaaattc
    18601 ggcatgcagt tgctggacga gcacctattg cagttgtacc aggatcacat aatctcggcc
    18661 gaggaggcca tcgaccacgc gcgcaacccg ggcgagatgc aggacaagat cgaggctatc
    18721 cagaagggtt tgccgcctga ggctggtccc ggcggcggcc cggggggtaa accgaagggg
    18781 ccggacggag agccggacct accgccgatg ccgaaaggat gaagaggagg ctgttagact
    18841 tttagacctc taggcttcta gaccgttaga tgccccatcg gggcatgatg ccgaacagtc
    18901 taatagccga acagccgaat agtctggaag atcctgacca tggctgagcc gctgaagaaa
    18961 acgcaggaga cacccgaccg caagccggca acggccatcg gcgcgggcat ggagaaaccg
    19021 ccgaccaaac ccccggcgcc gcccgcggat cgtccgcgtg ggccggggag caggatgccg
    19081 ccgatcagcc agttgcgagg ccgcacgctg ggccgaattc tgatcaagat gggcaagctg
    19141 acccgtacgc aggtcggcga ggccctcaac attcagaaga agaaccccgg accgatcggc
    19201 cagatcctgg tggagctcgg gtatgtcgac gaatcggacg tacagatcgc gctggccgcc
    19261 caggtgggca tggagccgat cgatctgaac aagatcgacg tcccgaagga ggtgctggct
    19321 ctggttccgg cgaaggttgc tcacacctac aagatcattc cgatcgatta tgagccttcg
    19381 acgaaaacgc tctcgatcgc gatggacaac ccggccaact tccaggccac cgatgacctg
    19441 aaaacgctga tggggctggc gatcaaacct tatctgtcaa accccgacga cattgccgcc
    19501 agtctcaaca agtactatcc cgaggatcag gccgagtcga tcagcgacct gatcaacgag
    19561 ctggccgatg atgaggacct cgccaagttt gccgaccgcg gcgacggcat cgacctggaa
    19621 gagctcaaag aggtggcgga gtccaacccg gtcaagaagc tgctcaacct ggttcttctg
    19681 caggccatcc gagacaaggc gagcgacatt catttcgagc ctttcgagga cgagtacaag
    19741 atccggtatc gcatcgacgg cgtgttgtac gagatggtgc cgcccccccg gcacattgcg
    19801 gtggccattg ccagccgtat caaggtcatg gccaacctga acattgcgga acgccgtctg
    19861 ccgcaggacg gtcgtatcga gttgacggtc ggcggcaacc cggttgatct gcgcgtgtcc
    19921 gttctgccga ccatgttcgg cgaaagcgtg gtgatgcgtg tgctggaccg cagcaacgtg
    19981 aagctgagcc tttcgcggat cggtctgcgg gacgatgaca tgaacatctt ccgccagctc
    20041 atcgccaagc cgaacggcat catcatcaac accggcccga ccggatcggg gaagacgacg
    20101 accctgtatg cggcgctggc ggagcttaat gacatcgaga ccaagatcct gaccgccgaa
    20161 gacccggtgg agtatgacat cgacggcctg tgccagtgcc aggtcaaccc ggacatcggg
    20221 ttgacgtttc accggtgctt gaggagtttt ctgcgtcagg acccggacgt catcctggtc
    20281 ggcgagattc gtgacctgga aacggccaag atagccgtgg aggcctcgtt gaccggacac
    20341 ctggtgttta cgacgcttca tacgaacgat gccccgtcgg ccgtggcccg gctgatggac
    20401 ctggggttgg agccgttctt gttgacggcc acgctggagg cggtggtcgc gcagcggctc
    20461 gttcggagga tctgcgagaa ctgcaaggcg gagtttcacc cgacggaaga gatgctgatg
    20521 gagctggacc tcaggccgga ggacgtgcgc ggcaaggtgt tcatgtacgg ccggggatgc
    20581 gactactgca acaacaccgg ttaccgggga cggatgggct tgtttgagat gatgctgatg
    20641 aacgacgatc ttcgggacct ggtgatgaag cacgcgtcga cgaacatact gcggaacgcg
    20701 gcccgcaaaa tggggatgcg gacgttgcgg gagtcgggct tgctggcgat ttatgagggg
    20761 atcacgacca tcgaagaggt agtgaaagag acgattatgg aggagatgta aggggtccgg
    20821 gatatgcggc ccgcggccgg tcgttccggc ccgggcggag acgcgtgacc ccggggtctt
    20881 cccgcgtgga ggctgaccat gccgaccttt caatacgagg ccatgaacca ggccggccag
    20941 gaagtcaagg acgagatcga agctcagtcc gtggaagacg ctctggcgaa gatcaggcaa
    21001 cagggctact acccgaccaa gctccgccag aagggcggca agcgggggca gaaagccgcc
    21061 gaggcgggcg gtgccggcgc gaagggtaag aagaagacgg ttggcgggtt cgggaaggtc
    21121 aagagcaagg agatcgtgca gttcactcgt caactttcga cgctgcagga cgccggtctg
    21181 ccgatcctgc gttgccttcg gattctggag cagcaacaga agccgggcaa gctccgcctg
    21241 atccttcgga acgtggcgga cgacgtcgag ggcggctcga cgctttcgga ggccatggcc
    21301 gcccagccca aagcgttcga ccgcctgtac tgcaatatgg tggccgcggg tgaggccggc
    21361 ggcgtgctcg acgtgatcct gcaacgtctg gccgacttca tggaaaaggc ccagcggctc
    21421 aagagaaagg tgatgggcgc gatgatctac ccgatcgcgg tgatcacctt cgcgatcggc
    21481 atcgtgtcgg gcctgatgat cgcggtcatc ccgaagttca agacgatttt cgccgacttc
    21541 gggacgtcgc tgccggctcc gacggtggca ttgatcagca tttcggagtg gttcgttcat
    21601 ggaacgccgc caggatgggt gatcgtgttc ctgtcgccgg tggggctcat gctgctggtc
    21661 aaactgatca aacaatcgga gtccgggcga ttcgcgatcg acacgatcaa gctgaagatt
    21721 cctattctgg ggaacatcct ggggaaatcg tcggtggccc ggtttacccg gacgctggga
    21781 actctgttgg cggccggcgt gcctatcctg gaggcgatta atatcacccg cgatacgtcc
    21841 ggcaatgagg tctatgcccg agccctcaag tcggttcatg acgacatccg tgagggcgag
    21901 tcgttcgcca acccgctgcg ggcggccaag atctgcgaca acatcgttat caacatgatc
    21961 gacgttggcg aggagaccgg cgatcttgac aagatgctga tgaagatcgc cgataactac
    22021 gatgaggaag tcgaaacgct ggtcgacggg ttggtcagcc tgcttgagcc ggtgatcgtt
    22081 atcgtgctgg gagcgatcgt cggctttatt gtcatcgccc tgttcctgcc gctggtcgag
    22141 ttgatcaact cggtttcttc gggcgggcag aaggagggcg tgtgaaccgg cgcgtccgcc
    22201 cggggccatc ggagccgaga gtggcctgca ggatggcctg ttcggggtct ggagagatgt
    22261 aagctcttgg gcctcagtgg tttatcgcga cgggggtggc tgtgtgcggg accgagcttg
    22321 ggtcgggcaa gcccggtccg gacggtgact tggctgcgat ggtgtgctac catcaggtgg
    22381 ggcggggtta ttgggatgcc tgcccggcct gcctcgaagc ggccataagc ctgcggtcgc
    22441 aggttctcaa taaggagcgt cacgatgact tgccgcgtct gtgtttgtcg ccgcaggggt
    22501 ttttccctcg tcgaactcct ggtcgtcgtc ggtattattg cgttgctggt ggcgatcctg
    22561 gtcccgtcgc tcaatgtggc ttcccggaga gccaaatccg cggcaacgct gaacacgatt
    22621 cgcgtcctgg agacgggttt ggaaaccttc aaggcggacg tggtggtcgg cggggcgtat
    22681 ccggaatctg cagtcgaagt gcctctcggt cgcggctcta tcaccgcggc taatccccac
    22741 ccgtctggcc tcaccggcaa agtgcccttc aatggggcct cgtaccttgt ttgggcgctg
    22801 gcgggagctg atctgcttgg gacgcccggt ttcctggatt tgaccggaaa cggcagttgg
    22861 cgcgacgaca cgggatactc gggaacacac gctctttatc agatcgaccc gaatagtagg
    22921 aggccgatcc atcctcgcta tggtcctttt gttgccgtcg aaagtatgag attggcccga
    22981 tggaacgata aggaaaagtg cttctttgtc gagaaggata tcagtcgtac cccgttgcct
    23041 tctacgtgtt tccttgacgc gtttgaccag ccgattctgt actatcgggc gaatccggcc
    23101 gggacactca ttgcaggggt gcagacagct ctgtcgcccg gggccgcagg gctgggcatc
    23161 tacgatctgt acgacaactt cacgtttacc ggtgtcgtca acccggtgga caaccaattc
    23221 ggtcccaggt tgaataggcc gcagggtctt gattttgggg ccggtcggat ccatccgttg
    23281 ggccagttgg gtaacccgct caatcccggg gccacgctcc gcacgttttc ctatatgatt
    23341 tgggacagca acgtgcttgc cttgccgcgt ccgcagcgtc cggacacgtt tatcctcatt
    23401 tcggcggggc cggatcacct gtacggcacg cccgacgaca tcggcaacat ccagatcaag
    23461 aagaagtagc gcggccggcg attacgcagg actcatgtgg ttgcgattgc tgcacgctcg
    23521 cctctgctgc gcgcggcagg gaggggattc gtgcgtggtc cggggcttgg acgggggtat
    23581 aatcttggag gtttgacgcc ggtccggttt tgtcgatgag gtgaggttgc gggtgagcga
    23641 cgcaggttta caggcgaagc tggagagaat gaaggaggtt ctccgcgggt tggagcgggt
    23701 tgcggtgggc ttttcggcgg gggtggattc gacgttcctg ctgaaggtcg cggtggacac
    23761 gcttgggccg gccaacgtgg tggccgtaac cgccgacagc gagagtctga cccgccggga
    23821 gctggatgag gcccggaagt tggcggccca gatgggtgtc gagcacgtgg tcgtccgcac
    23881 ggatgagctg gatgacccca actaccgggc caacccggcc aaccgctgct attactgcaa
    23941 gcaagcgctg tttcggaggc tagacgagtt catcgccggg cggggggcga tggtcatggt
    24001 catcggcgtg aatactgacg atttcgccga ctggcggccg ggcctgcggg ccagcaagga
    24061 gaaaggcgtt cgcatgcccg cggccgaggc cggcatgagc aaggctgatg tgcgtgagtt
    24121 gagccgccag atgggtttgc ccacccacga caagccctcc agtccgtgtc tggcgtcccg
    24181 cctgccgtac ggcgaggaga tcacgcccga gaagctcagc caaatcgagc ggtcggaagc
    24241 cttcttgcac tcactcggtt ttcacgtctg tcgcgtgcgt catcacggca acatggcccg
    24301 gatcgaggta cctgccgatc agatcgagcg attgtgcgaa cctgcgacac gggccaaggt
    24361 tgacgccacc ctccgagagt tcggctacca gtatgtggcc gtcgacattc gtggcttccg
    24421 cagcggaagt ctcaacgaga tgctgtcccc gtcgcaaacg ggcgttcaag ccaagtaggg
    24481 cagcgtcgag cgacgcggga cgtgtcattg ggcagaggcg gagggatgaa ggcagaaggc
    24541 tgaaggcgga aggcgaaagg ctgaaggcgg aaggatgaag gaggaaggat gaaggatgag
    24601 ggaggaagga tgaaggatga gctgaggagg cgaagctcgg cggctacgtt gcggtatcga
    24661 ctcatccttc cgccttcatc ctttcttccc aaggcggacc gcctttttgc gggtcgcaac
    24721 ccaagcgctc tgcgatcaag aaacatgcgc gcaagccgca cagatgtctg gccgtaaggt
    24781 tctgaaccct gaaccctgaa ccctgaaccc ctgtcccgtg ctcaatacac ttgcaaccgc
    24841 ggcgagtgta tccccatctc ctccagtgtc ctgtgtatct ggaggcagtt ttgctccggc
    24901 tgattgaggt ccaggtggta ctcgaactgc agccgggcgt cgccgttgtc ggcgacacat
    24961 tggcggaccc aggcgtccat gtcctggggg cttccggcgg tgagcagatg ggtttggggc
    25021 atgatgtcga tgcggatggg cccgaaccgg tctcggacgg cggcgacgct ggtgccgctt
    25081 ccgaggttca ggatggcaac gtttcggact tcgcgaaaga cgtcgagcag gtgattggag
    25141 ttgccacagg agtggagtcg cagcggcccg acgcgatcgg ccagattgtt gaggcagggc
    25201 agggcgaact cggcgaactg gtccggcccc atcatgcagc cggagcactc gccggtgtgc
    25261 aggccggtga cggtgatccc ggcggcgtcg gcgaagagct gaatcagctt gacgtaggcg
    25321 tcaacgatcc aagcgaacag ttcgcggacg aagcccgggt tgtcggccat ttcgagaaag
    25381 acgcgttcgc cgatcagttt ttgggcggtg gtgatgatgc cgtgggtggt ggcccggcct
    25441 gtggtgtccc aaaagaaggg cgggatgaca gccacatccg ggccgagcgt ctgtcgcatc
    25501 tcgtggactt ggcccagcag gaggctgacg ggccaagtgt ccggccaatc gatgttccac
    25561 aagggaccga cgtcggtcag cccggccagc ggttcgggtg agatatccgg gtctttgtcg
    25621 ggcgcaatga taaaacgggc cccaacggcc gcaccgagga tgagatttgg ctgtaggccg
    25681 ccgaccaagg ccaccggccg tcgccggcct tcgacctgca cgaggtgggc ctcgatgctg
    25741 tagatgggca ggtcgggaaa ccgctgggcg acgaaggcct gcatccggcg ctcctgttca
    25801 agtcggcgcc gcgggtcgaa aaagaagccc tcgtcgaaca ggaacccccc atgcgcgtgc
    25861 agccagccga agcttacact gccgaggacg gacatgtgcc cagtgtcagg catagtttgt
    25921 ctctgtcgcc gggtcgcatc acctctggcg actctggcct gccccgaagg accgtggttt
    25981 cattcagggg gtcgcgctct cgggcgcagg aatcggccgg cctgtgacga ggctgtacag
    26041 aacatcaggg cagagatcgg ctccgttcgg ccactggatg gttccgagct ctgtgttgac
    26101 cgtgacctgc cgaaagtaac cgaggtcttc gaggggggcg aagacgccgc cctgccccac
    26161 aacccagtcc tgaaggttgg cctctccctg tgtgccgtca gtgaaaacga gtctgaggcg
    26221 atactggcga atcggggtgg cttcggttgt gcgaggaaac atggtgacct tcggcataca
    26281 actcctttct gtacagccat tatactgtgc caacaaccgt caagtaagga agggcgcaag
    26341 gaagacgggt ggaacgtcga attggagaac ggtctaaccc agtggcagct ttaccgcttg
    26401 cctctgcctc gccgagatct ccgccgcgaa gcagatttcc atcgtttcgg ccgcatcctc
    26461 gatgtcgggc atcggccgtc ggccggcatt aagcgcatcg atcaactcgg ccacctcgcc
    26521 gtcgaacggg tggtgcgata cgtcgccgga gtcgggcagg atcgtcggga tagtcgcgaa
    26581 gccggtctgg ccgtcgtaga ggtcgccggc gaactggttg ttgcggaagc tgccggctgt
    26641 tccgtagacc tccacgttaa acacatacgg caatttcgcc tccaggcagc aaccgacctt
    26701 gccgatgctg ccgtcgtcga atttgagcag ggccagttcg gtgcttgggt agtcgagtgc
    26761 attggtgcgg ccggcggagt acgccgacac ttccaccact ggccggccga ccaggaagcg
    26821 aatcgcgtcg atggcgtggc agccgccgat caggagactg gttccggccg ttgcccgcgt
    26881 ggtgtgccaa ggtttggtgt agggcttgcc gacgatgccg tgcagatagt cggtctctgc
    26941 gaagatgatc cggccgagac ggccttccgc gatgtttctg cggatgatct tgagcagggg
    27001 gttccagcgc aagacgaagc cgatgatggt tttcacgccc gcctcgcgca ccgccgcccg
    27061 tatggctttc atgtcctcca atgtcaggca gaccggcttt tcgatgatga tgtgcttgcc
    27121 ggccttggcg gccgcgaggg tgattggggc gtgaagatga tggggtgtga acacgccgac
    27181 gatgtcgatt gagggatcct tcagggcggc acagagatcg tctgagcagg ggcaattcag
    27241 gttatgctca cgggtgaacg cggcggcgcc ctcgtacgtt cttccgacga cgtagccgat
    27301 tcggacatcg tcccgcttgc agaatgattt cgcgtaagcc ccggccaccc atccaggccc
    27361 aacaaccgca acgttccagg tcttcttcgg cacaggtctg tcctctcatg atgtcggctg
    27421 tcatcggcag gtctgcgccg ccttcggcga ctccgacctg ccctacgttc
//
LOCUS       DAOTPW010000042        27285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1841_length_27285_cov_4.947962, whole genome shotgun sequence.
ACCESSION   DAOTPW010000042 DAOTPW010000000
VERSION     DAOTPW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27285
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_27285_cov_4.947962"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2803
                     /locus_tag="P5316_05725"
     CDS             <1..2803
                     /locus_tag="P5316_05725"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRR84492.1"
                     /translation="GCPVHGTAIYQYGTYPWRLHPTNRFKVICPVGGESYPSNDFDAY
                     YRSGMTDTSLLAGKYVDSGRGYVAPDGEKYWFVAYACHSHWANDWLPAVTWLAQAYAL
                     TGQRVYAEKAIVMLDRIAEVYPAMDYDKQSRYGEMSSATYPGKILNRGWEMQTLRDLV
                     LAYELVFDALIDPGSVSLPWRSAANIRANIEANLLEEAVDVLDGWQDGNSIGIRQNTL
                     VHTMLVRQHGPTASLLDQLLSSPGGAAQQQGINYALHNLVYKDGMPHEGSPYYCFVWV
                     RELTELGLPLLRANIDLFAHPKMQRMLDTPLEMICIGQFTPAVGDAGTINDGWIGPIT
                     PTYHAAYQQLGRPQYAWALDQLGELRDGWTHYFEDCLRGAIDLAALQSDLDSYQPIQP
                     SRLLDGYGMAILNNPQNRLAVSVFHGLRGSHAHADRLNIELFGRNRRLSPDLGYPDAM
                     NEYVAGIYSWSKNTVSHNTLLVDDRKQDGNVAGTVLRFHESPTAHAVDIDAAGTYAKT
                     DIYRRTLVLVDVDNDNSYLVDVFRARGGSKHVLSIHGPEGQFTMTGTTLSAPVTQGTL
                     AGANVAYGQLYDDPVLGAPGYGGPYSTYMGSGYSHLFNWQHAAPSTPITAQWRTTDNL
                     AGLRVHVPDHAGQDVIVADAYVSPLRKIPTILKYMLVRRSADAPGNTFVSVWEPTGSQ
                     SFIQSVNLSNAASLGQGSDQIVVLTVQRAGATDTIVVSPEAGKAFTLPSGLSCDAAVA
                     VVTVSNGQLTRGFAAGGTGLTMSQPPVEIAVPATITGSISAADYENKAVTMTCDQPPA
                     NAAALVGKMVRFHNAAHSCMYTIASARASGNTLVLGLEGSEVFTGRVSIRNADLLART
                     VTTPTKVPHPANLAGMRLLTDDLSAQAMIVSMSSTGTIQLAAGSNMAAFASSLPKDAW
                     IADFGYGDDVEIEMSVHMP"
     gene            complement(3009..4232)
                     /locus_tag="P5316_05730"
     CDS             complement(3009..4232)
                     /locus_tag="P5316_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR84493.1"
                     /translation="MHVIYIHQHFSTRKGATGTRSYEMSRRLLAAGHRVTMICGAYGH
                     GDLGGDTAERLAEHDIDGIRVFRIAEPYSNQMSFMRRTMAFGRFARAAAQIACGLDGD
                     LVFATSTPLTVGLPGMKAGRRLGVPFVFEVRDLWPELPIALGVVRNPLTKAYLRRMER
                     RIYRAAHRIIALAPGIKEGICRTGYSADQVAMIPNCADLDLFRPTDEPLSDPRFGGPV
                     DLKLVFTGAHGLANGLDAVLDAAAVLRDRGEKGIRLVFIGDGGQRERLMRRSQNEGLD
                     ALISWLPPMPKEELADVLPRMDAGMMVLKNVPAFYYGTSPNKFFDYIACGLPVLNNYP
                     GWLADMVTANRCGLVVRPDDPEAFADAVIWMRDHRPELKEMGRRARELAEREFGRDRL
                     ADQFVRTLEEARASF"
     gene            complement(4436..5599)
                     /locus_tag="P5316_05735"
     CDS             complement(4436..5599)
                     /locus_tag="P5316_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR84494.1"
                     /translation="MIDSSARSVREYQRAFAESLGVPRAFAFWKGRVALYAILKALGI
                     GEGDEVIMPGYTCVMAVNPLVYLGAKPVFVDIEPRTYNINPDFIEAKITPRTRLIIAQ
                     HTYGYPARMDAITDICQRHGLTLVEDCCLALGSRYKGKLAGTFGVAAYFSFQWNKPFT
                     SGLGGMAVVHDRELADRIASVVGQGLAQPTMREVAMLRAQLMIYRTAIYPRTTAFAQN
                     VFRWLTRKGLVVGSSSTCEFQPRMEPDFFKGMSQVQAHVGLRQLARLDNNIEHRRRMT
                     RVYDSLLSERGWSVTQLPSEMEPVLVRYPVRVADKQRALAEAASHFVELGSWFECPLH
                     PIETPMEAYGYHTGMCPEADRASRETVNLPLHPRADEATARRTVDFICTIGQA"
     gene            complement(5596..6276)
                     /locus_tag="P5316_05740"
     CDS             complement(5596..6276)
                     /locus_tag="P5316_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR84495.1"
                     /translation="MKVSDREILQWDVANWRVVLQLWDETAVCRDDAQVLDLGARDGG
                     LSLYFASKGCRVVCSDLNGPSPVAKVLHERYGVGHLVKYEAIDATNIAYPEASFDIVC
                     FKSVLGGIGGAGGYTAQEKAVREMHRVLRPGGKLLFAENLVGTRLHAFLRKRFVAWGE
                     RWRYLSLGEVHDLLRPFSEVTRVCTGLLATFGRREWQRSLLHFVDVLVDPITPDAWKY
                     IVAGCAVK"
     gene            complement(6371..7477)
                     /locus_tag="P5316_05745"
     CDS             complement(6371..7477)
                     /locus_tag="P5316_05745"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR84496.1"
                     /translation="MQVPLLDLRAQYAKIKDEILAAVSEVLDSQICIGGPKVAELEKA
                     IAAASDCRFAVGTSSGTDAILCSLMSLEIGPGDEVITTPFTFFATVGCIARTGARPVL
                     VDIDPQTYNISPALIEEVVTPRTKAIIPVHLFGQMADMDPIIDIARRHNLHVIEDAAQ
                     SITATYKGRKAGSIGTVGCFSFFPSKNLGGIGDGGMCVTNDEALFNRLMVMRNHGSQP
                     KYYHKYIGGNFRLDPIQAAALLVKLPHLNAWSEARRRNALLYTRAFAGSVVQTPYVNP
                     DCVSIFNQYTIRMPKRDEMVQHLRAHEIGCEVYYPVPAHLQECFACLGYKRGDFPESE
                     KASLEVLSLPIYPELTEEQISYVANTVLDFVERG"
     gene            complement(7538..8224)
                     /locus_tag="P5316_05750"
     CDS             complement(7538..8224)
                     /locus_tag="P5316_05750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRR84497.1"
                     /translation="MSGSGMREQAGERGSFFVHESSYVDDGAVVGQGSRIWHFSHLMK
                     GCRIGRNCNLGQNVFVAAGVEIGDNVKIQNNVSLYTGVIVEDDVFLGPSCVLTNVSNP
                     RSQVNRQSLYERTLVRRGATVGANATVVCGVTLGRYCFVGAGSVVTRDVPDYALMVGV
                     PARQKGWVSRHGVPLSPADANGIMTCPESGYRYQETSAGVLRCLDLDEETPLPADKSV
                     GTRSYRSYRP"
     gene            complement(8239..9177)
                     /locus_tag="P5316_05755"
     CDS             complement(8239..9177)
                     /locus_tag="P5316_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84498.1"
                     /translation="MKRFALIGAAGYIAPRHMKAIKETGNVLVAALDKSDSVGILDSY
                     FPDADFFTEFERFDRHIELLRRKGEGVDYVSICSPNYLHDAHVRFALRVGAHAVCEKP
                     LVLNPWNATALAELAAEANRQVNTILQLRLHPAIAGLRDRILSAPKDKMFDVDLTYVT
                     SRGHWYMASWKADVAKSGGVATNIGVHFFDMLSWIFGEPRSSIVHISEPKRMAGCLEL
                     ARARVRWFLSIDRRDLPEPPAAGKPATYRSITVDGAEVEFSEGFRDLHTVSYAQILEG
                     RGFRPEEVVPSIEIVSAIRHAKPVGLVGEYHPMLKR"
     gene            complement(9317..10351)
                     /locus_tag="P5316_05760"
     CDS             complement(9317..10351)
                     /locus_tag="P5316_05760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR84499.1"
                     /translation="MTVQENPFVSVIVAVRNEADFIERCLSSILDGDYPRDLMEVLVV
                     DGMSDDGTREIVQRLSARDARIRLLDNEGRIVACGLNAGIRAARGQVITWLGGHAEYA
                     PDFLRQSVETLKAHADCWCVGGPIETVSETYVGKVTAAAMSTPVGVGNAMFRLGGYEG
                     YVDTATFASYWRWVFDKIGLFDEELVRNQDDEFNARLIRQGGKIWLTPLIRSRYYPRT
                     SLGKLWRQYFQYGTWRIRTIQKLGQPATVRQVAPLLFVVGLVACLVGAAVWRPLTWLF
                     AAYVALYSLGLLVGVWQVARQAGIRCALFSPVVFMILHFGYGLGSLKGIWLFVIRKRG
                     GRYGDKSLSR"
     gene            complement(10449..11483)
                     /locus_tag="P5316_05765"
     CDS             complement(10449..11483)
                     /locus_tag="P5316_05765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR84500.1"
                     /translation="MKKRVLVVSTFLREAGLPPSTCYVLTSLLTQAGFEIVTTSNKQG
                     RLSRLADMIVTAWRARHQYAAACVDVFSGPAFIWAEAVCWTLRRARKPYILMLRGGDL
                     PLFASAHKGRVRRLLQSASTVVAPSRYLQEKMQPYRDGIRVLPNGIHLSAYRFRLRSS
                     PRPRLAWLRSLAETYNPCMAVRVVAELSVEWPDLELVMAGADKGDGSMQQVERLAAEL
                     GVQSRVKLPGQVPKERVPDLFERADIFLNTTNIDNTPISVLEAMACGLCVVSTDVGGI
                     PYLVQNGHDALLVPPDDAPAMAEAVRRLLRQPAAAETLSRNARQTAERCDWSRIIPQW
                     QSIFAEVGGW"
     gene            complement(11574..12959)
                     /locus_tag="P5316_05770"
     CDS             complement(11574..12959)
                     /locus_tag="P5316_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84501.1"
                     /translation="MNVMRWVYDHSPLFWQNVMCSVSGAVRQWQRYGRGFEERLAFFR
                     EAAKWSRAEAERYQAAKVREMLQAAYDAVPFYQHRFDQVGFRPDEFRTLEDLARLPLL
                     CKDEVRRAGKTLIAAGYRGRRLVVSHSGGSTGMPLTCYHDREAMIDLYASFWAYQRPG
                     VQLGDRYATFQGLELIPPRQTTGPYWRMNRAASQRLYSIFHLSEHTISEYIEDLDRFK
                     PVYVQGYANSLYLLAKLAREKGIDPKWAPKAVFSTSEQLLPVYREVIESVFRTKVWDA
                     YSQDEGCGSITQYECGYYHYDRAYGYMEFLDQAADGTRKLAEIICTGFLNKAWPLIRY
                     RVGDMVDYEDVETCPKCGRPGPIIHEIRGRTGDVLITPSGRAFPHISLIVKNLHGVRQ
                     VQLVQEAKDRIRIRFVPSEDFRESEDEHLILSSFAKAIHEPVDWSLERVEEIPRTRSG
                     KFMSIVSNISP"
     gene            complement(13304..15181)
                     /locus_tag="P5316_05775"
     CDS             complement(13304..15181)
                     /locus_tag="P5316_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84502.1"
                     /translation="MPGMIGGIGLLSARSASLQGQFESLQREFRETWGDYELINLPTA
                     FLAGHSFVPGTAVHTTRDGVQFAVDGESAIYVHAHRFAEEGRPELYCLEKEELGLRPH
                     AKGNVAILAEQGQVLYLITEHSASFPLYYAEVEGGLLFSSLMRPLGRAVGAEPDPAGV
                     VQYCWVGYHVNGRTHFKGVRGLLPGQALIYEAATGRMQIRETSRAWLKDLSHMRPAEI
                     VEQACETLSRAVDRTCMPELRHALMMSGGWDSRLALAAMRKKLSPSHVQGYLHGDPAS
                     RELALGLQICRRLKVPCHLQPVEEDSFQLAAMPRDFARSESLEWFYWHRAACLLASRG
                     INCIGTGMWGEVMGGGFGTATLLKGSSSLRHLACLGGHLLNVGRVSPRLLIDHGWGIK
                     QVHGHLRLSFKNRPWTANVTCFTDLPGITEEINADVMAHLKRIEARGIDDPSRMIEAF
                     ITETKGGQRMTAQLRCLRAYVDVANLFADQEFFYLGSWVPYRYKIHCLLFREILRRVE
                     PALLRYSTAATLVPAGSPILLQEASRVMRRVFDDAATRLVARSRVNLGIRPSGYMNLE
                     FLRDGRLFTALIDSLRTQFLDKSVLRDVADQVVHRQIADVPNNVSKGFLRVYAADLFL
                     R"
     gene            complement(15248..16675)
                     /locus_tag="P5316_05780"
     CDS             complement(15248..16675)
                     /locus_tag="P5316_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84503.1"
                     /translation="MTGLGQRAYWLAPYFLKRWMASWSARRLDGQRYGPEYDAVLSEI
                     RERDGWSAEQFAAYQNEALRRIVQLAGEHVPYYQRRFAEAGISPQDIRGVEDLSRLPI
                     LEKNVVREFPTQLLNQTLDRASLILLHTSGTTGTPLDLYRDRRLSATACAYFDARCHA
                     VAGMQRRVNRSVSIGGHLVAPPWRTRPPFWVYNARWRQLYMSSSHLAPQYLDAYVDAI
                     REFAADYIEGYPSSVYAIARHIVDQKLEPVRMKACFTTAETLLEYQRDAISGAFCCWT
                     YDQYGCGEMAVFAAECREGSLHLSPEYGIVEVLDAQDRPVLPGEVGQLVCTSLVNHVQ
                     PFLRYRLGDLGCLLPGRCRCGSEMPMLGQIEGRIDAVLVTRDGRRIGRLDPVFKGARG
                     IVEAQVVQDDLDRFRIRIVPAKDYTDNDGRVVAANLAHRIGGGDIRVEVVPAIERTAA
                     GKFQAVVSLLPRPSAAAASASKHDG"
     gene            complement(16672..17868)
                     /locus_tag="P5316_05785"
     CDS             complement(16672..17868)
                     /locus_tag="P5316_05785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR84504.1"
                     /translation="MDRLSGHHPARSDGSQGSDDRSRVLHVGPLPPPVGGMATNVEAF
                     LCSDVARSFAIRNVRVDLIGKSRYRGFLRKLANLINAAVLTAVVASMVVVWRPVLVHV
                     RTNSWGGFFEKAFLVLLARLGRCRTILQVHGGAFAEFYDQAGWLGKKAIRRVLAVPDR
                     VIAISREMQGTLCRVSSRPDRVTVVENAVFVPQRTIWDVKEAAGSQRGQAPVKILFLN
                     RVEPAKGIWELLEALGTVLERSNACARIVGPEPDCGGELMSRIGQLGLQDRIRLEPAV
                     QGQAKEKAYLSADIYVLPSHVEGMPLGLLEAMSYGLPCIATPVGGVPSVIDHDRNGLL
                     VPPKDPRALAAAILRLTQDASARRRLGSVARQTIVDRFSWDRRAKEFVSLYRDVLETA
                     TSDRRR"
     gene            complement(17872..18984)
                     /locus_tag="P5316_05790"
     CDS             complement(17872..18984)
                     /locus_tag="P5316_05790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR84505.1"
                     /translation="MIPQIGVGGAERQLLELIVRSDRAVVEHQVLYYNDARDTDQMAA
                     YQRLGVAPARVPRNRARPWRFIRDLAAMIRAARPDVVHCWMGSGAVWGRFAAMLAGAR
                     NIIVVYRGTRLSRVYMQRLNERLTGRRVHYMSNSRACARIIAEQLHLVAAKFHVVYNG
                     VDASRYQVPSDRAALLSELGLPDDRKLVVTVARLTPVKDYPTLLRAARRCRGQLPVRF
                     LIVGHGELDAELRAVASQWDLDDTVHFLGLRHDIPRILRSSDLFCFTSLSEGFPNALL
                     EAMAAGLPIVTTDFPGAEEVIDHGSTGFVVRSGDDEAVYSAIRELVEKPDPGREMGDR
                     ARQRAQGSFSMEAMVRNTLSYYRTILNGSARPTAGG"
     gene            complement(19163..20473)
                     /locus_tag="P5316_05795"
     CDS             complement(19163..20473)
                     /locus_tag="P5316_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84506.1"
                     /translation="MINPNSMVYAQERVWVAPFAPRPISAASAYDQPPSLGRKCVELF
                     YYLSVVNSVFVYKTGAAMLPYFGGALVVMAGAVCLTIMLAQRERIPLTVWCAVLINVF
                     ATLSQGIRGEIPIIGEGLNQLFLWACQSLVICYLVRNPGTQKRLLLFFAIVLVFCVYF
                     SGELQGGSSGRRLRLERYGVGSSFGNANDLAYMVGTFAVAVLFWSLRSIRSVRPLLWA
                     LALVLLVITVRTVSRGAITAVGCGLAMLAVAIMLARGARIGGIVMVVFGIILLSQLSF
                     LLADPMEFLKARMKGDGSSRVRLAVYNEATLEQLAETYLFGQGIAGTKSVVTGVTPHN
                     TYLYTQFAYGGLAAVSYLAWMLAMAIRTTRVCFRSELRMDLRLEVVALFGMTLMSHLL
                     SNQGFVFLSSMYAVGLIEKYAVRLPRDVWVGQAGWPDRQSAGLG"
     gene            complement(20510..21649)
                     /locus_tag="P5316_05800"
     CDS             complement(20510..21649)
                     /locus_tag="P5316_05800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR84507.1"
                     /translation="MRIAVVSPSFLPSIGGAEFVAHHLARNWSMNGHEVCVMNAISDQ
                     PPENETSYNVKRFTILRGSTRFGYHRFPFSGYATRQLDRLISDYKPDFISAHFGYPTG
                     VWLSRISRLPTTLVTCHGRELTKFDWGYRSVHGIDSILARALNSLTRSVAISTHARKL
                     MEELGVVSNKIVDIPNGVEWGKFQEKATFDLRKKLQLPRDAIVVLTVGRQHQQKDYET
                     GLRAFAQLAAGTRGVYYVILGRGTACWQRLAEELGVSTRVRLCEGLHGIELIGAYQQA
                     DVFFSPSIWEMFPLVVIEAMAAGLPAVVTNISGSQDAVKTNVNGIIVEPRQPGQMAEA
                     LGRLTADAGVRHQMGQASRELAERYDWRHISRMYIDCVKSNAETA"
     gene            complement(21652..22704)
                     /locus_tag="P5316_05805"
     CDS             complement(21652..22704)
                     /locus_tag="P5316_05805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR84508.1"
                     /translation="MARALRSLGVDVRFVVGRKVASVDQPLDEFETEYIRSPYLRDVE
                     YRYSGSPWRILRAASLRARRMDDRLFQMAALKRLLADKQADVFQVCALADLAARLTEA
                     GRKAVVRWTGPPSGYMAGWAVRCSGSFSHGESYEAAKRCGLNPAHIVAGCDTELFRPP
                     VRREYLRDRCEFIFVGRCVPVKNLEFLLSGFAEARRRSHGLRLSIVGDGDSLPGIRKR
                     IAELGIADSVRLPGFLHGADLAQQYQAADCFVLVSLYESFSLVALEAMSSGLPLILSR
                     VGHLPRFVEQHQAGSLVEPGDVQGLADEMLRWQEQVEERRRTGENNRRVIEEQYSWKS
                     SARKLLDYYESLCRKA"
     gene            complement(22798..23640)
                     /locus_tag="P5316_05810"
     CDS             complement(22798..23640)
                     /locus_tag="P5316_05810"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HRR84509.1"
                     /translation="MTDRIFFSLRDDDTSYFTRPEHLERCYKRIWRICPISLSVVPFH
                     GCTKSGALPREYWRGDRMFPLASNGELVAFLRENMSHGRIHVTLHGYHHRDEVRGFEF
                     VAGADLDRKVRDGRQYLESLLGQPVTVFVPPHNALSWEGYRAVVRAGLHISGIQSFHP
                     SFRGWDPRIAWLGLKRRWWYGQHGCEWPWPLVFPDGHREVPHLTVSPSVGLDHVMRAF
                     RSVLKTGGVFCLATHYWEFDAPSRCGSVTVREMVEQLWEEAQQHVDRIEFLPLGRLAE
                     VNRP"
     gene            complement(23664..25493)
                     /locus_tag="P5316_05815"
     CDS             complement(23664..25493)
                     /locus_tag="P5316_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRR84510.1"
                     /translation="MFVLGTCQSRGQIGLHQWLTIVQLASGASTRPGIHLNQELLGLT
                     CVLGDGLASGVCVGAAESQSGLTIAGVVTTGAWAIRNRQGSQANAVGDFAARLAERYE
                     DEGDGIFADLDGAFLAVVGDIRSRKVVLARDHVGQFPLYYALLDHGIAFSSQAHLLLD
                     GPVVGRHLDREGAAQYLLFGFLMGNKTLVKGIALLEAGCALHFQPEAGKSEVRRYFVL
                     PSGDEEPNPAAAIDQIDDSLRTACRGAAAAGDVPAICLSAGLDSRVLLAITHREGLDL
                     ACVTNGVQGGIELELTSRMCRSVGHKHIKCLFGPDLVEKASDWLSGVVEASDGEASLL
                     DIMMLYVARKYRHELGISHVIRGHGGELTKLERAYSFAVPAELALGSDHVAVRDHLYR
                     QLIASCEGVDLCAISAADWRDVMLDGPRSTFCASYAKWEGSVYPGQCVSILFITEFLS
                     RRMMSSMRCLRTELAVSLPFLDPKVICAILRSPLRLRLGTTMQNLLVSRNHAGLLTIP
                     NSAMRVPLTASPLRRQLVSYAYRIARRLGYGRADVPERWIERRLANLFDTVLLSSQAR
                     SRDFVQSQAVSKLVRRYAEGEKRLAGFLGRMTMLELWMRRFMD"
     gene            complement(25599..25724)
                     /locus_tag="P5316_05820"
     CDS             complement(25599..25724)
                     /locus_tag="P5316_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84511.1"
                     /translation="MGLKSYVNRIKRFRRVATRYEKTARNFMSFVQLAALFCWMT"
     gene            25784..27223
                     /locus_tag="P5316_05825"
     CDS             25784..27223
                     /locus_tag="P5316_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HRR84512.1"
                     /translation="MQATAEPMRVEGLGAGLTEEQAREIYRQGEEAVVFALLEQAKLL
                     AEAQANGSAPPHPMSPSTPSGMRPVYTKPPGSRRQKRPGCKKGHPGARRSRPDRIDQR
                     REHRLECCPHCQGPLHRCHQTRTRYTEDIPENIQPVVTEHIIHRDWCPRCKKHVEPVV
                     TEALPGSTLGHRVLVLSAWLHYALGNTLSQIVEVFGHHLQLELTPGGLVQMWHRLAEI
                     LGGWYEQIRDEALRAGVLHGDETGWRVNGKTRWLWCFSTVDLTFYMIDRCRGSPALLR
                     FFQQAFGGTLVSDFWGAYNAVACSKRQKCLVHLLRDLEHVETYRPVNQDWPAFAKKLR
                     RLIGDAIRLWKREAVPPAEYASRRARLDRRLQELIETRWTHPDARRLIKRLRRHHNEL
                     FTFLDEPGVPFDNNHAERAIRPAVIIRKNSQSNRSDRGAETQAILMSVYRTLKQRGYP
                     PIRTIVDAIRSYLTTGQLPPLPAKIAASG"
BASE COUNT         5927 a         8711 c         7501 g         5146 t
ORIGIN      
        1 aggctgcccg gttcacggca ctgcaattta ccagtacggg acatacccat ggaggctgca
       61 cccgacaaat cgcttcaagg tcatttgccc ggtcggcggg gagagctacc cgagcaacga
      121 ctttgacgcc tattaccgaa gcggcatgac ggacaccagc ctgctggctg gcaagtacgt
      181 cgacagtggc agaggctacg tcgcacccga cggcgagaag tattggtttg tcgcttacgc
      241 gtgtcacagc cactgggcga acgattggct acctgctgtc acctggctgg ctcaagcgta
      301 tgcgttgacg ggtcagcgcg tctatgccga aaaagcgatc gtcatgctcg acaggatcgc
      361 cgaggtctac ccagcgatgg actatgacaa gcagtcgcga tacggggaga tgagtagcgc
      421 aacctaccca ggaaaaatcc tgaatagagg ctgggagatg caaacgcttc gggacttggt
      481 actggcatac gaactcgtgt ttgacgctct catagatccg ggctcagtct ctttgccgtg
      541 gaggtccgct gcgaacatta gagctaacat tgaggccaac ctgcttgagg aagccgttga
      601 tgttctcgac ggctggcaag acggcaacag catcggaata cgccagaaca ctttggtcca
      661 caccatgctc gtacgtcagc acggtcctac agcgagcttg cttgaccaac tcctgagttc
      721 tcccggcggc gctgcccaac agcagggcat caactatgct ctccacaatc tggtttacaa
      781 ggacggcatg ccccatgagg gctcacccta ctactgcttt gtctgggtaa gggaactgac
      841 agaactcggc cttccgctgc tcagagcgaa tatcgatctg ttcgctcatc caaagatgca
      901 acgcatgctc gacacaccac tggaaatgat ctgcatcggc cagttcacac ctgccgtggg
      961 tgatgccggt accataaacg atggctggat cggtcctata acgccaacct accatgccgc
     1021 gtatcagcag cttggtcggc cccaatatgc ctgggctcta gaccagctgg gcgaactccg
     1081 cgacggttgg acgcattact ttgaggattg tctccgtggc gccatcgacc tggccgcact
     1141 ccaatctgat cttgattcat accagcccat acagcctagt cgattgctgg atggctacgg
     1201 catggcgatc ctcaacaatc cacagaatcg ccttgcggtt tctgttttcc atggattgcg
     1261 aggctcgcat gcccatgccg atcgcttgaa catcgagttg ttcggtcgga atcgaaggct
     1321 ttcaccggat ctcgggtatc ccgatgcgat gaacgagtat gtcgcgggaa tctacagctg
     1381 gtccaaaaac accgtcagcc acaacaccct gcttgtcgat gaccgcaagc aggacggcaa
     1441 cgtcgcgggc acagtgctgc ggttccacga aagcccgaca gcccacgcgg tcgacatcga
     1501 cgccgccggc acctatgcca agaccgacat ctatcggcga acactggtgc tggtcgacgt
     1561 cgataatgac aactcctacc tggtggatgt tttccgtgcc cgtggcggca gcaagcacgt
     1621 gctcagcatc catggcccgg aaggacagtt caccatgacc ggcaccacgc tctctgcccc
     1681 ggtcacccag ggcaccctgg ccggcgcaaa tgtcgcctac ggccagctct acgacgatcc
     1741 agtcctcggc gcaccggggt atggtggccc gtacagcaca tacatgggca gcggctactc
     1801 acacctgttc aactggcagc atgccgcgcc gagtactccg atcaccgccc agtggcgtac
     1861 aaccgacaac ctcgccggcc ttcgcgtcca cgttcctgat cacgctggac aggatgtgat
     1921 cgtcgccgat gcctacgtgt ccccgctccg caaaatcccg acgatcctta agtacatgct
     1981 ggtgcgccga tccgccgatg cgcccggcaa tacatttgtc tctgtttggg agccaacggg
     2041 aagccaatcc tttatccagt cggtgaatct cagcaacgcc gcctcactcg ggcagggtag
     2101 tgaccagatc gttgtgctta ctgtgcagcg ggcaggggca actgacacga tcgtcgtttc
     2161 tccggaggct ggcaaggcgt tcacgctgcc atcgggcctg tcttgcgatg cggccgtcgc
     2221 agtggtcacc gtctcaaacg gacagctcac ccggggcttc gctgccggcg gcacaggtct
     2281 gaccatgagc cagccgccag tggagatcgc cgtccccgcg acgatcacgg gctcgatctc
     2341 cgcagccgac tacgaaaaca aggccgtgac gatgacctgc gaccagcccc ccgccaacgc
     2401 tgccgcactc gtcggcaaga tggtgcgatt ccataacgcc gctcactcct gcatgtacac
     2461 gatcgcctcg gcacgggcct ccggcaacac gctggtcctg ggtcttgaag gctcggaagt
     2521 cttcactggc cgggtcagca tccggaacgc cgacctgttg gcacggacgg tcaccacgcc
     2581 caccaaggtt ccgcatccgg ccaaccttgc gggcatgcgg cttctgacgg atgatttgtc
     2641 cgctcaggcg atgatcgtct cgatgagcag cacggggacc atccaactgg ctgccggcag
     2701 caacatggct gcattcgcca gcagccttcc aaaggacgcg tggatcgctg atttcggcta
     2761 cggcgacgac gtggagatcg agatgtcagt tcacatgccg tagcaaacgg aagtgaatca
     2821 ggacgctgtg gcggcgaggt tcagagaggc ggatcggggc cctatggcga tcagtgccat
     2881 ctggtgcgac ctcgaccggc cgttggtgag gtggtgtgga aatggattac tagcaggctt
     2941 cttcgcagac cggaaacagt cgcgaatatg gagaggtggc atgagacggc gtgcggagtg
     3001 ccgacagctc agaagcttgc ccgggcttct tccagcgtgc gaacgaattg atcagccaga
     3061 cgatcgcggc caaactcccg ctcggccagc tcccgggccc gccgtcccat ttccttgagt
     3121 tccggccgat gatcgcgcat ccagattacg gcgtcggcaa aggcctcagg atcatcgggc
     3181 ctgaccacca agccgcagcg gttcgcggtg accatgtcgg ccagccaacc ggggtagttg
     3241 ttcagcaccg gaagcccaca ggcgatgtag tcaaaaaact tgttgggcga cgtcccgtag
     3301 tagaatgccg gaacgttctt gagaaccatc atccccgcgt ccattcgcgg cagaacgtca
     3361 gccagttcct ccttgggcat cggagggagc caactgatca gtgcgtcgag cccctcgttc
     3421 tggctccgcc gcatcagccg ctcgcgctgc cccccgtcgc cgatgaacac caagcggatc
     3481 cccttttcgc cccggtctct taacacagct gctgcgtcga gcaccgcgtc caggccgttg
     3541 gcaaggccgt gggccccggt aaaaacaagc ttgagatcga ccggccctcc gaaccgagga
     3601 tcactgagcg gttcgtccgt cgggcgaaac agatcgagat ccgcgcagtt cggaatcatc
     3661 gcaacctgat ccgccgaata acccgtgcga cagattccct ccttgatgcc cggcgccagg
     3721 gcaatgatac ggtgagccgc tcggtagatc cgtcgctcca tcctgcgaag gtacgccttg
     3781 gtgagcgggt tgcgaacgac gcccaaggcg atgggcagtt ccggccacag atcacgaacc
     3841 tcaaacacga acggcacgcc caggcgtctg cccgccttca tgccgggtag ccctacggtc
     3901 agcggcgtcg aggtcgcaaa gacaagatcg ccgtccaggc cgcacgcaat ttgcgccgcc
     3961 gcacgggcaa agcggccgaa tgccatggtc cgccgcatga agctcatctg gtttgaatag
     4021 ggctcggcga tgcgaaacac tcgaatacca tctatatcgt gctcggccag tcgctccgcg
     4081 gtgtcgcccc cgaggtctcc gtggccgtat gcgccacaga tcatggtcac ccggtgcccg
     4141 gcggccagca accttcggct catttcatac gagcgggtgc cagtggctcc cttgcgggtc
     4201 gagaaatgct ggtggatgta gataacgtgc atgatcagct catgccctcg gtcattcccg
     4261 cgcggaccgc cacctgattc tgcccatggg gcagacgcaa cgccacacgt ggatgtctgg
     4321 attcaggaaa aacagaaggg agagatcgga gaccgttggg accgccggat catagcaaca
     4381 gcgacgaagg tcaatcgagg aacactcctg ttcggttgtc cattcgctgc caagatcacg
     4441 cttggccgat cgtgcagatg aaatcgaccg ttcgtcttgc ggtggcctca tcggcgcgcg
     4501 gatgcagggg taagttgacg gtctctcgcg aggcccgatc cgcctcgggg cacatgccgg
     4561 tatggtagcc gtacgcttcc atcggcgtct cgatcgggtg cagcggacac tcaaaccaac
     4621 tgcccagttc cacgaagtgg gaagccgcct ccgccaaggc gcgttgcttg tcggcgacgc
     4681 gcaccgggta gcgaaccagt actggctcca tttccgacgg caattgcgtc accgaccagc
     4741 ctctctcgct cagcagacta tcgtacacgc gggtcatccg ccggcggtgc tcgatgttgt
     4801 tgtccagtct tgccaattgc cgaaggccga cgtgggcctg cacctggctc atgcccttga
     4861 aaaagtcagg ctccatacgg ggctggaatt cgcacgtact cgatgaaccg acgaccagcc
     4921 cctttcgcgt cagccatctg aatacgttct gggcgaatgc ggttgttcgc gggtagatcg
     4981 ccgtgcggta gatcatcaac tgtgcccgga gcatggcgac ttcacgcatc gtcggctgtg
     5041 ccaagccttg tccgacgaca ctcgcgattc gatcggcaag ctcgcggtcg tggaccaccg
     5101 ccatgccgcc caggccgctg gtaaatggct tgttccactg gaaggagaag taggccgcga
     5161 caccaaacgt cccggcgagc ttgcccttgt accgtgagcc cagagccagg cagcagtctt
     5221 cgaccaaggt cagcccatgc cgctgacaga tatctgtgat ggcgtccatt cgggccggat
     5281 acccgtaggt gtgttgggcg atgatcagcc tggtgcgtgg cgtgatcttc gcctcgatga
     5341 agtccggatt gatgttgtac gtgcgaggct caatgtcgac aaaaaccggc ttggcaccca
     5401 gatagacgag cgggttgacg gccatcacgc aggtgtagcc gggcatgatg acttcgtcgc
     5461 cttcaccgat acccagggct ttcagaatcg cgtacagcgc aacgcgacct ttccagaagg
     5521 caaaggcacg gggtacaccc agagactcag caaaggcgcg ctgatattcc cttacactgc
     5581 gggcagaaga atcaatcatt tcaccgcgca ccccgccact atgtacttcc atgcgtccgg
     5641 cgtaatcgga tcaaccagaa catccacaaa gtgtagcagg cttcgctgcc attctcttcg
     5701 tccgaacgtc gcaagcaacc cggtgcagac ccgcgtgacc tcggaaaacg gtcgcagcaa
     5761 atcgtgtacc tcgccaagcg acaggtatcg ccatcgctca ccccaggcga caaatcgctt
     5821 tctcaggaag gcatgcagac gagtgccaac aaggttctcc gcaaagagca gcttgccgcc
     5881 cggtcgcaga acgcgatgca tctctcgaac agccttctct tgggccgtgt aacccccggc
     5941 cccgccaatt ccgcctagaa ccgacttgaa acagacgatg tcgaacgagg cttcaggata
     6001 tgcgatgttc gtcgcgtcga tggcctcgta cttgacgaga tgcccaacac cgtatcgctc
     6061 gtgcaagacc ttggcgaccg gagacggacc gttcaggtcg gagcaaacaa cgcgacaacc
     6121 cttcgatgcg aagtagaggg aaagcccgcc gtctctggct cccagatcaa gaacctgggc
     6181 gtcatcacga caaaccgcgg tttcgtccca aagttgcagc accacccgcc agtttgcgac
     6241 atcccactgc aggatttcgc ggtccgagac cttcacgagc cgcttgcctc cctcgcaaaa
     6301 cacccgacat tccccgaatc aggacctctt cgggcatcat cataatgtgg gccattcatc
     6361 gagcagccct tcaccctcgc tcgacgaaat cgagcacggt gttggcgaca taactgatct
     6421 gttcctcagt cagttccgga tagatgggca gcgacagaac ttccagagaa gccttttcag
     6481 attccgggaa gtcgcctctc ttgtatccca ggcaggcaaa gcattcctgc aaatgcgcgg
     6541 gaaccggata gtagacctca cagccaatct catgcgccct gaggtgttgc accatctcgt
     6601 ctcgcttcgg catacggatg gtgtactgat tgaaaatcga cacacaatca ggattcacgt
     6661 acggcgtttg caccactgac cctgcgaagg cgcgcgtgta cagcaacgca ttccgcctcc
     6721 tggcctccga ccaggcattc agatgcggca gtttgaccag cagcgccgca gcttgaatcg
     6781 gatcaagccg gaagttgccg ccgatatact tgtgatagta cttaggttgg ctgccatgat
     6841 ttcgcatgac catcaggcga ttgaacaagg cctcatcatt ggtcacgcac atgccgccat
     6901 cgccgatgcc gcccagattc ttgctcggga aaaagctgaa acacccgacg gtgccgatgc
     6961 ttccggcctt gcgtccctta taggtggcgg taatcgattg cgctgcatct tcaataacgt
     7021 gcaggttgtg gcgccgagcg atgtcaataa tcggatccat gtccgccatc tgaccgaaga
     7081 ggtgtaccgg gatgatggct tttgtccgcg gtgtgacgac ttcttcgatc agggcagggc
     7141 tgatgttgta cgtctgcggg tcaatgtcca cgagtacagg tctggcaccc gttctggcga
     7201 tgcagccgac ggtagcgaaa aaggtgaacg gcgtcgtgat gacttcgtcg ccagggccga
     7261 tctccaggct catgaggctg cagaggatgg catccgtgcc gctggaggtc ccgacggcga
     7321 accggcagtc gctggcggca gcaatggcct tctcaagctc ggcgaccttg gggccgccga
     7381 tgcatatctg ggaatcaagc acttccgaaa ccgcagccag gatttcgtcc ttgatcttcg
     7441 cgtactgcgc ccgcaaatcc aatagtggaa cctgcatcag tcacccgcat gtgaaagcat
     7501 aactggaacg tagcagccgt tcacaaatca gacgcctcta tggccggtac gaccggtacg
     7561 accgcgtgcc cacggactta tcggccggaa ggggcgtctc ctcgtccagg tcaaggcagc
     7621 ggagcacgcc cgcagaagtc tcctggtacc ggtagccgct ctccgggcac gtcatgatgc
     7681 cattcgcatc ggccggcgaa agtgggacac cgtgccggct cacccaccct ttttgacgag
     7741 cgggcacgcc gaccatcaat gcatagtccg ggacatcacg cgtcaccacc gatcctgccc
     7801 ccacaaaaca ataccggccc agtgtcacgc cacatacaac cgtcgcattg gctccgaccg
     7861 tggctcctcg cctgaccaag gtgcgctcat acaggctctg acggttcacc tgggaccgcg
     7921 ggttgctcac gttcgtcagc acgcaggaag gcccgaggaa gacgtcgtct tccacgatca
     7981 caccggtgta tagcgagacg ttgttctgga tcttgacgtt gtcgccgatc tcgacacctg
     8041 ctgcaacgaa cacattctgc cccagattgc agttccgtcc tatacgacag cctttcataa
     8101 ggtgagagaa gtgccatatg cggctgcctt ggcccaccac cgcaccgtcg tcgacgtagc
     8161 tgctctcatg gacgaagaaa ctgccgcgct caccagcctg ttcgcgcatg ccgctgccag
     8221 acattcgaac accccttact atcgtttgag catcgggtga tactcgccca ccagccccac
     8281 cggcttggca tgtctgatgg cagaaacgat ctcgatcgaa gggaccactt cctcaggacg
     8341 aaaacctctg ccttccagga tctgtgcata gctcacggtg tgaaggtcgc gaaacccttc
     8401 ggagaactcg acttccgcgc cgtccacggt gatggaccgg tacgtcgccg gcttgccagc
     8461 cgccggcggc tcgggcagat ccctccgatc aatcgagagg aaccatcgca cacgagcccg
     8521 ggccaattcc agacagcccg ccatgcgctt cggctcagag atatggacaa tgctgctcct
     8581 gggttcgcca aaaatccacg acaacatgtc aaagaaatgg accccgatat tggtggccac
     8641 gcctccggac ttggccacgt cagccttcca cgaggccata taccagtggc cgcgggaggt
     8701 cacgtaggtc aaatcgacat cgaacatctt gtccttcggt gccgacagaa tgcgatccct
     8761 gagccccgcg atcgccggat gcagccgcaa ctgcaagatc gtattcacct ggcggttggc
     8821 ctcagccgcc agttcagcga gcgcggtcgc gttccacggg ttcagaacga gcggcttctc
     8881 gcaaacggcg tgcgcgccga ccctgagagc gaaacggaca tgggcgtcat gaaggtagtt
     8941 tggcgagcag atcgacacat agtccactcc ctcgcctttg cgtctgagca actcgatatg
     9001 ccggtcgaac cgctcgaatt cagtgaagaa atccgcatca gggaagtacg agtcgagtat
     9061 gcccaccgaa tcgctcttgt ccagggccgc cacgaggacg ttgcccgttt ccttgatcgc
     9121 cttcatatga cgcggcgcga tgtaaccggc cgctccgata agagcaaagc gtttcattct
     9181 cacaccttgt ggtcagaggc cgttattcgg ccgtccacta cctgaaggcc ctctcattcg
     9241 gaagcctccc ttgcagacac acgtccgcgc cctcggcacg cacgcctcaa ttcatgcacg
     9301 aaggctccga gcgattctac cggctcagag acttgtctcc gtagcggcca ccccgcttgc
     9361 gaatcacaaa cagccagata cctttcagag agcctagacc gtacccgaag tgcagaatca
     9421 tgaacacgac cggcgagaaa agagcacagc gaatgcccgc ctgccgtgcg acctgccata
     9481 caccaacaag aaggccaaga ctgtagagcg cgacgtaggc cgcaaacagc caggttaaag
     9541 gtcgccagac agcggcgccg acaaggcagg cgacgaggcc caccacgaac agtagcggcg
     9601 cgacctgccg aaccgtcgcc ggctgtccca gtttctggat ggtgcggatg cgccacgtcc
     9661 cgtactggaa atactggcgc caaagcttac caaggctggt tcgcgggtag taccggctac
     9721 ggataagcgg cgtgagccat atcttgccgc cttgccgaat caggcgggcg ttgaactcgt
     9781 catcctgatt gcgaacgagt tcctcgtcaa acaagccgat cttgtcaaac acccagcgcc
     9841 agtacgaggc gaaggtggcc gtatcgacat agccctcgta tccgccgagc cgaaacatgg
     9901 cgtttccaac accgaccggg gtgctcatgg cggcggccgt aaccttcccc acataagttt
     9961 cgctgaccgt ctctatcggt ccgcctacgc accagcaatc agcatgagct ttaagcgttt
    10021 cgaccgactg cctcaggaaa tccggggcat actcggcgtg cccccctagc caagtgatta
    10081 cctgccctct ggccgcgcgt atgcctgcat tgagtccgca tgccacaatg cgcccctcat
    10141 tatctaacag cctgatcctc gcatcccgcg ctgacagccg ctgcacgatc tcacgcgttc
    10201 catcgtcgct cattccgtcg acaaccagga cttccatcag gtcgcgaggg tagtctccgt
    10261 caagaatgct gctcaggcat cgttcaatga agtccgcctc attgcgcacg gcgacaatca
    10321 cagacacaaa cggattctct tgaaccgtca ctcaacctcc ttcagtgtct ccgtgtcgcc
    10381 catcgcgatc gccaatacac acgccgcgct tccgcccgat aggtctcggg aacgattcct
    10441 tgtggacctc accatccgcc aacctccgca aagattgact gccactgagg aatgatgcgg
    10501 gaccaatcac atcgctccgc cgtttgccga gcattccgcg acagggtctc cgccgcggcg
    10561 ggctgcctca gcagccgccg cacggcctca gccatggcag gagcatcatc gggtgggaca
    10621 agtagcgcat cgtgcccgtt ctgcaccaag tacggtatgc cgcccacatc tgtactcacc
    10681 acacacagac cacacgccat ggcttcaagg acactgatcg gcgtattgtc gatattcgtc
    10741 gtgttcagga aaatgtccgc cctttcaaac aggtccggca ccctctcttt cggcacctga
    10801 cccggcaact tcacacgact ctgaacaccg agctcagcag ccagtctttc gacctgctgc
    10861 atgctcccgt ctcctttgtc cgccccggcc atgaccagtt caaggtcggg ccattccacc
    10921 gaaagctcgg ccaccactcg gacggccatg cagggattgt aggtttcagc aaggcttcga
    10981 agccacgcca gtcgcggacg cggggaggat cgaagcctaa aacggtatgc agacaggtga
    11041 attccattcg gcaaaacgcg gatgccatcc cggtagggct gcatcttctc ctggagatac
    11101 cgcgaaggtg caacgaccgt agaggcagat tgcaggaggc ggcgcactcg acctttatgg
    11161 gcagaggcga acaagggcaa atcgcctcct cgcaacatca gtatgtatgg ctttctagct
    11221 cttcgcaacg tccagcatac ggcctccgcc catatgaagg ccggaccact gaacacatcg
    11281 acgcaggccg ccgcgtactg atggcgcgca cgccatgccg tcacgatcat gtctgccaac
    11341 cgcgacaacc gaccctgctt gtttgatgtc gtgacgatct cgaagccggc ttgcgtcaga
    11401 agcgaggtta atacgtaaca ggtcgacggt ggaagcccag cctcacgcaa gaaagtgctg
    11461 acaaccagga ctctcttctt catgcgtcct gttccctggg tatggcgttg tggaacctac
    11521 tgaccagccc tttggtggcc cggccaaacg gacgagaaaa ccacggtttc gtactacggt
    11581 gagatgttgc tcacaatcga catgaacttg cccgatcggg tccgcggtat ctcctcgact
    11641 ctctccaagg accagtcaac cggctcgtga atggccttgg cgaagctgga tagaataagg
    11701 tgctcgtctt cagattcacg aaagtcctcg ctcggcacaa agcggatcct gatccgatct
    11761 ttcgcctcct gaaccagttg cacctgcctg accccgtgca ggttcttgac aatcaggctg
    11821 atatgaggga aggcacggcc gcttggtgtt atcagcacgt ctccagtgcg cccgcggatc
    11881 tcgtgaataa tcgggcccgg ccggccgcat ttggggcagg tctccacatc ttcataatcc
    11941 accatgtctc cgacgcgata cctgatgagc ggccaggcct tgttgagaaa acccgtgcag
    12001 atgatttcgg ccagtttccg cgtgccatcg gccgcctgat ccagaaactc catatagccg
    12061 taagctcggt cataatggta gtagccgcac tcatattgag taatactgcc gcagccctca
    12121 tcctgcgaat aggcatccca cacctttgtt cgaaacaccg attcaatcac ctcgcggtag
    12181 acaggaagaa gctgctcgct cgttgagaac acggccttcg gcgcccactt gggatcaatg
    12241 cccttctccc gggccagttt ggccagaaga tacagcgagt tcgcatagcc ctggacgtac
    12301 acgggcttga agcgatccag atcctcgatg tactcgctga tcgtgtgttc gcttagatgg
    12361 aaaatgctgt agagcctctg gctggccgcc cgattcatcc gccagtatgg tcctgtcgtc
    12421 tgtcgcggcg ggatcagttc gagcccttga aacgtggcgt aacgatcccc taattgcaca
    12481 cctggacgct ggtatgccca gaaggacgcg tacagatcga tcatagcctc acgatcatga
    12541 tagcacgtca acggcatccc cgtgctgcct ccgctgtgcg ataccacgag tcgcctgccc
    12601 ctgtagcccg cggcgatcag tgtcttgccc gccctgcgaa cctcgtcctt gcacaagaga
    12661 ggcagcctgg ccagatcctc cagagtgcgg aattcatcgg gcctgaagcc aacctggtca
    12721 aaacggtgct ggtagaacgg aacagcatca taggcagcct gcagcatctc ccgcaccttg
    12781 gcggcctgat agcgctcggc ctcggctcgc gaccacttgg ccgcctctcg gaagaaggct
    12841 aacctctcct caaagccccg gccatatcgc tgccattgcc gcacggcacc ggaaaccgaa
    12901 cacatcacat tctgccagaa cagcggtgaa tgatcgtata cccaccgcat cacattcatt
    12961 gaacgatcct gcctttgcaa gtgatccgtg ctcgttcgcg atactccggc cccgggagca
    13021 ggtccgcact gtcgtgcatg gtggtaagcc cagtcgctcg cttcattcag agcctcaagc
    13081 tgctgtggcg aaggttctcg cacggaacgc ccgggcacgc aagagtcaac ctcagataga
    13141 ctccgagact attcagagcc tgcatccgtt cgtaaccgtc gctgcatgac aagccttgtg
    13201 gaagtaccat ttgcagatca ttcggaccgg gccgccgtcg acaacacacc acaatatccg
    13261 caacatcccg caggaatgag aacgtgacaa ccgccaccaa ccgctaccgc aaaaacaggt
    13321 cagcggcgta cacacgcagg aatcctttgg agacattgtt gggcacgtct gcgatctggc
    13381 gatgaaccac ttgatccgcg acgtcgcgta agacactctt gtcgaggaac tgtgttctaa
    13441 gactatcaat caatgccgta aaaagccggc catcacgaag gaattcgaga ttcatgtatc
    13501 ccgatggccg gatgcccagg tttactcgcg atctggcaac aaggcgcgta gcggcatcgt
    13561 caaatacccg ccgcatcacg cgagaagctt cctgtagcaa tattggagac cctgccggaa
    13621 ccaacgtcgc cgccgtcgaa taccgtaata gagcaggctc cacccgcctc agaatctcgc
    13681 gaaagagaag gcaatggatt ttgtagcggt aggggaccca actacccaga tagaaaaact
    13741 cctgatccgc gaacagattg gccacgtcga cgtaggcgcg caaacaacgc agttgcgccg
    13801 tcatgcgctg gccgcccttg gtctcggtga tgaacgcctc gatcatccgc gacgggtcgt
    13861 caattccgcg agcctcgatc cgcttcaggt gagccatcac atctgcgttg atctcctccg
    13921 tgataccggg caggtcggta aagcaagtga cgttggcggt ccacggccgg ttcttgaacg
    13981 aaagccgcaa atgcccatgg acctgtttga tgccccatcc gtgatcgatc agcaggcgcg
    14041 ggctgacccg ccccacattc agcagatgcc cccccaaaca tgccaggtgc cttagagaac
    14101 ttgagccctt gagcaacgtt gccgtgccaa atcccccacc cattacttcg ccccacattc
    14161 ctgtgccgat gcagttaatg ccgcgactcg ccagcaggca cgccgcgcga tgccagtaga
    14221 accattccag actctcggaa cgggcgaaat cccgtggcat cgcagccaat tgaaagctgt
    14281 cctcctcgac gggctgcaaa tggcaaggaa ccttcagacg acgacagatc tgcagcccca
    14341 gcgccagctc tcggctggct gggtcgccgt gcaggtaacc ctgtacatgc gaagggctca
    14401 gcttctttcg catcgccgcc agcgccagtc tcgaatccca ccccccggac atcatcagcg
    14461 cgtgccgcaa ttcaggcata caggtccgat cgactgccct actcagcgtt tcgcaggcct
    14521 gctcaacaat ctccgcaggg cgcatgtgtg acaggtcctt cagccaggct cgtgaagtct
    14581 cgcgaatttg catcctgcct gtcgcggcct cgtagatcag tgcctggccc ggaagaagcc
    14641 cgcgcacccc cttgaaatga gtccgcccgt tgacgtggta cccgacccag caatactgaa
    14701 cgactccggc cgggtccggc tccgcaccga cggctctgcc caatggccgc atcaggcttg
    14761 agaacagcag tccgccctcc acctcggcat aatagagcgg aaagctggcg ctgtgttcgg
    14821 tgatgaggta gagaacctga ccctgctccg caaggatggc aacattgccc ttggcatgag
    14881 gtcggagccc caattcctcc ttttcaaggc aatacagctc cggtcgtcct tcctcagcaa
    14941 accggtgggc gtgaacatag atcgccgatt cgccatcgac cgcgaattgt acgccgtctc
    15001 gcgtcgtgtg aacggccgtc cccggcacaa aagaatgtcc cgccaggaaa gccgtcggca
    15061 ggttaatcag ttcgtaatcc ccccatgtct cgcggaattc ccgctgcagc gattcgaact
    15121 gaccttggag cgacgcagac ctcgccgaca gaagcccaat tccgccgatc atgccgggca
    15181 tgaggtctcc cttatgcgca gccccgtgat caggaacatc agagactcac caggcgacat
    15241 gagccgctca accatcatgt ttgctcgccg aggccgccgc tgccgaaggc cgaggaagca
    15301 gcgagacgac tgcttgaaac ttgcctgccg ctgtccgctc gattgccggc acgacctcca
    15361 ccctgatatc gccgccgccg atcctgtggg ccagattggc ggcaacaacg cgtccgtcgt
    15421 tgtccgtgta gtccttggcc ggcacgatgc ggatacgaaa ccgatcgaga tcgtcctgga
    15481 ccacttgggc ttccacgatc cctcgagccc ccttgaacac cggatcgagt cgtccgatac
    15541 gccggccgtc gcgagtcacc agcaccgcat cgattcgccc ctcgatctgg cccagcatcg
    15601 gcatttcgct gccgcaccgg catcgtccgg gtagcaggca tcccaggtcg ccgagccgat
    15661 atcgcaggaa cggctgcacg tggttgacca ggctggtgca gaccagttgg ccgacctcgc
    15721 cgggcagcac cggccggtcc tgtgcgtcaa gcacctcgac gatgccgtac tccgggctca
    15781 ggtggaggga cccttcacgg cactcggcgg cgaacaccgc catctcgccg caaccgtact
    15841 ggtcgtaggt ccagcagcaa aacgccccgc tgatcgcatc tcgctggtac tccaacagcg
    15901 tctcggccgt tgtgaaacaa gccttcattc gcaccggttc gagtttttgg tccacaatgt
    15961 gccgggcaat cgcgtacacg ctcgacgggt aaccctcgat gtaatcggcg gcgaactcgc
    16021 ggatagcatc cacataagcg tccagatact gaggcgccag atgcgagctc gacatataca
    16081 gttgccgcca ccgggcgttg tagacccaga acggaggccg agttcgccac ggcggggcaa
    16141 ccaggtgccc cccgatgctg accgatcgat tgacccgcct ttgcatcccg gcaaccgcat
    16201 gacatcgggc gtcgaagtag gcacaagccg ttgcgctcag ccgccgatcg cgatacagat
    16261 ccagcggagt gccggtcgtc cctgaagtgt gcaggaggat cagcgaggct cggtcaaggg
    16321 tctggttaag cagttgggtc gggaactcgc ggacaacgtt cttttccaga atcggcagac
    16381 gcgaaaggtc ctcgacaccc ctgatgtcct gaggtgagat gccggcctcg gcaaaccgcc
    16441 gttgatagta cggaacatgc tcacctgcca gttgaacgat ccgccgcaaa gcctcgttct
    16501 gataggcggc gaactgctcc gcactccagc catcgcgctc cctgatctcg gacagtaccg
    16561 cgtcatactc gggtccatat cgctgaccgt cgagccggcg ggcgctccac gaggccatcc
    16621 accgcttcag gaaatagggg gccagccaat aagctcgctg tccgaggccg gtcatcgccg
    16681 cctgtctgac gtcgccgttt ccagaacatc ccggtagagg ctgacgaact ccttcgcccg
    16741 ccggtcccac gagaaccgat ccacgatcgt ctgccgggcc accgatccca agcgccgccg
    16801 ggcagacgcg tcttgcgtca aacggagaat cgcggccgcc aaagcccgcg gatccttcgg
    16861 gggaacaaga agcccgttgc ggtcatggtc gatgacgctc ggcacccctc cgaccggcgt
    16921 ggcaatgcag ggcagaccgt aggacatcgc ctcaagcaga ccgaggggca tgccttcaac
    16981 atggctcggc aggacgtaga tgtctgcgct cagataggcc ttttccttgg cttgcccctg
    17041 gacggccggc tccagccgaa tccgatcctg aaggccaagc tgcccaatcc gactcatcaa
    17101 ctcgccgccg cagtcgggct ccgggcccac gattcgcgcg catgcgttgc tccgctccag
    17161 gacggtgccc aaggcctcca gcaactccca tatgcccttg gccggctcga cccggttcag
    17221 gaacaggatc ttcaccgggg cttggccgcg ctgggagccc gcggcttcct tcacatccca
    17281 gatcgttcgc tggggcacaa agacggcgtt ctcgacgacc gtaacccgat ccggacgcga
    17341 agagacgcgg cacagtgtgc cttgcatctc gcgagagatc gcgatgacac gatcgggaac
    17401 tgcaaggacc cggcggatcg ccttcttgcc gagccaaccg gcctggtcgt agaactcggc
    17461 gaaagcgccg ccatgaactt gaagaatggt gcggcagcga ccaagcctcg ccagcagcac
    17521 aagaaacgcc ttctcgaaga agcctcccca tgagttcgtt cgcacatgca ccaagacggg
    17581 ccgccacacc accaccatcg aggccacgac ggctgtcaga accgccgcgt tgatgaggtt
    17641 cgccagcttg cgaagaaagc ctcggtagcg ggatttcccg atcagatcca cgcgaacgtt
    17701 gcggatcgca aatgacctgg ccacatcgga acacagaaac gcctctacat tcgttgccat
    17761 cccgccaacc ggcggcggca gggggcccac gtgcagcacg cggctccgat catcgctccc
    17821 ctgtgaaccg tcactgcggg cgggatgatg gcccgacaac cgatccacac gtcatcctcc
    17881 cgccgtcgga cgcgcagatc cgttcaagat cgtcctgtaa tacgacagcg tgtttcgcac
    17941 catggcctcc atcgaaaacg agccctgcgc tctctgcctg gcccggtcgc ccatttccct
    18001 gcccggatcc ggcttctcga ccaattcccg aatcgccgag tacaccgctt cgtcatcacc
    18061 cgaccttacg acaaaccccg tgctcccatg atcgatgacc tcttcggccc cggggaaatc
    18121 ggtagttacg atcggcaggc ccgcggccat cgcctcgagc agagcgtttg ggaatccttc
    18181 actcaggctg gtgaaacaga agaggtccga cgaacgcaga attctgggaa tatcgtggcg
    18241 aagcccaagg aagtgcaccg tgtcgtcaag atcccattgg cttgctacgg ctcgcagttc
    18301 cgcgtcgagt tctccgtgtc cgacgatcag gaaccggaca ggcagttgcc cccgacaccg
    18361 ccgagctgct cgcaacagcg tcggataatc cttaaccggt gtgagccggg ctacggtcac
    18421 aaccagcttg cgatcgtcgg gcagccccag ttcactgagc agcgccgccc ggtctgaggg
    18481 aacctggtag cgcgaggcgt cgacgccgtt gtagaccaca tgaaacttcg cggccacaag
    18541 gtgcaactgc tcggcaatga tccgcgcgca ggcccggctg ttcgacatat agtgaacgcg
    18601 gcgcccggtc agtctctcgt tcaaccgctg catgtacacc cgggacagtc tcgtgccccg
    18661 gtaaaccacg atgatgttcc tcgcaccggc cagcatcgcc gcgaaccgcc cccagaccgc
    18721 tccgctgccc atccagcagt gcacgacatc cggccgtgcc gctcggatca tcgccgccag
    18781 gtcccggatg aaacgccacg gccgtgccct attgcgaggc acgcgcgccg gcgctacgcc
    18841 cagacgctga tatgcggcca tctgatccgt gtctcgggca tcgttgtagt acaacacttg
    18901 atgctcgacc acagcacggt ccgatcgcac gatcaactca aggagctgcc gctcggcgcc
    18961 gccaaccccg atttgaggga tcacgtggag aatacggagg ggagtcaatc gaattcgttg
    19021 cctcgtgctg acttacagcg gggtctccgc tcgccgatac gcaggcccgt tcgtggcggg
    19081 tgaatctcgc cgagccaccg gatgccactg gaggctgtcc accagcgcgc gcaattgacc
    19141 taaagaagaa gtcattcgct tctcagccaa gtccagcaga ctgcctgtcg ggccaaccgg
    19201 cttgccccac ccagacgtca cgcggcaggc gaacagcgta cttctcaatc agacctactg
    19261 catacatgct ggaaagaaaa acgaaccctt ggttgctgag caggtgcgac atgagcgtca
    19321 tcccaaacag agcaacaact tccagccgca gatccatccg taattcgctc cggaaacaca
    19381 cacgagttgt acgaatagcc atcgccagca tccaggccaa gtagctgacg gccgcgaggc
    19441 cgccatatgc gaattgagtg tagagatagg tattatgcgg tgttactcct gtcacaacgg
    19501 acttggtccc cgcgatccct tgtccaaaca ggtatgtctc agccagttgc tcaagcgttg
    19561 cttcgttgta gaccgccagt cgcactcgac tgctgccatc gcctttcatc cttgccttca
    19621 ggaactccat ggggtcagcc aacaagaatg aaagctgcga cagtaatatg atgccgaaga
    19681 caaccatgac gataccaccg atcctcgccc ccctggccaa cataatggcg accgctagca
    19741 tggccagacc gcagccaaca gccgtgatcg cgccacggga aacagtccga acagtgataa
    19801 caagaagaac taaggccagc gcccacaata gcggccgcac agatcttatg ctgcgaagcg
    19861 accagaacag gacggcaacc gcaaaggttc ccaccatgta ggccaaatcg ttggcgttgc
    19921 caaaggacga acccacacca tacctttcca atcgcaagcg cctgcctgag ctcccgccct
    19981 gcagttcgcc ggagaagtaa acacaaaaca cgagcacaat tgcaaaaaac aacagcaacc
    20041 gcttttgtgt ccccggattc cgaacgaggt aacagataac cagcgactga cacgcccaca
    20101 gaaagagctg attcaacccc tctccaataa tgggtatctc gccccgtatg ccttgcgaga
    20161 gcgtcgcgaa aacgttgatg agtacggcac accacactgt caacgggata cgttcacgtt
    20221 gtgccagcat gatggtcaga cagaccgcgc cggccatgac cacaagcgct ccgccgaaat
    20281 acggcagcat ggcggcacct gtcttatata cgaagacact attcacgaca gacaaatagt
    20341 agaacagttc gacgcatttt cgccccagag aagggggctg atcgtatgcc gacgccgcag
    20401 aaatcgggcg cggtgcaaaa ggcgcaaccc agacccgttc ttgcgcatag accatgctgt
    20461 taggattgat cattgtacac tccgacctcg ccgtttcggc ttctctcccc taggctgtct
    20521 cagcattgct cttgacacaa tcgatgtaca tgcggctgat gtgtctccaa tcatagcgtt
    20581 cagccagttc ccgactggcc tggcccattt gatgacgcac gccggcatca gccgtaagtc
    20641 tgccaagtgc ttcggccatc tgacctggtt gtctcggctc tacgattatt ccattgacat
    20701 tcgtcttgac ggcatcttgg ctgccgctga tattcgtcac tacggcaggc aatccagcag
    20761 ccatcgcctc gatgaccacc aacggaaaca tctcccatat tgaaggggag aagaagacgt
    20821 ccgcttgctg gtatgcccca atgagttcga tcccatggag tccctcgcac agacgcacgc
    20881 gagtcgacac acccaattct tccgctaggc gctgccaaca cgccgtgcca cgcccgagaa
    20941 tcacgtagta cacacctctt gtcccggctg ccagttgggc gaaggcacga agaccggttt
    21001 cataatcttt ttgctggtgc tgacgaccaa cagtgaggac gacaatggcg tcgcgaggca
    21061 actgaagctt ctttcgaagg tcgaacgtgg ccttttcttg aaacttgccc cactcgacgc
    21121 cgttcgggat atccacgatc ttgtttgaca cgactcccag ctcctccatt agctttcttg
    21181 catgtgtgct gatggcaacg gaccgcgtca acgaattgag cgctcttgcg agtatcgaat
    21241 cgatgccgtg aacggaccga tacccccagt caaacttggt gagttcacgc ccgtggcagg
    21301 ttaccagagt tgtgggcaat cgtgagatgc ggctcagcca cacccctgtg gggtatccga
    21361 agtgggccga gatgaagtct ggcttataat cagaaataag gcggtccagc tgcctcgtcg
    21421 cgtatccgga gaagggaaac cggtgatagc caaatcgcgt cgaaccacga agtatggtga
    21481 atcgcttcac gttataagag gtctcgtttt cgggaggctg gtcactaata gcgttcatca
    21541 cgcagacttc gtgtccgttc attgaccagt tccgcgccaa atgatgagca acgaactcag
    21601 ccccgccgat tgatggtaga aacgaaggag acaccactgc aattctcata ttcaggcctt
    21661 cctgcagaga ctctcgtagt aatccagcag tttgcgggca ctcgatttcc aactgtactg
    21721 ttcctcgatc actcgacggt tgttctcgcc agtacgtctg cgttcctcga cttgctcttg
    21781 ccagcgaagc atctcgtccg caagcccctg tacgtcgccg ggttccacga gacttcccgc
    21841 ctgatgctgt tcaacaaaac ggggtaaatg acccactcgc gacaaaatga gcggtaatcc
    21901 ggagctcatc gcctcaagag cgacaaggct aaacgactcg taaaggctga ccaaaacaaa
    21961 gcagtcggct gcctggtatt gctgcgccag atccgcccca tgaaggaagc caggtagtct
    22021 cacgctatca gcaattccca attcggcaat cctctttcgg atacccggca acgagtcgcc
    22081 gtctcccact atcgagagtc gcagaccgtg gctccgtctt cttgcctctg caaagccgct
    22141 cagaaggaac tcaaggttct taacgggaac gcaacggccc acaaagatga attcgcaacg
    22201 atcacggaga tactcccgac gaacaggcgg gcggaataac tccgtatcgc aaccggcgac
    22261 tatatgagcc ggattcaatc cgcatcgctt cgccgcttcg tacgattcgc catgcgaaaa
    22321 actaccgctg caacgaacgg cccagccagc catataaccg gacggtgggc cggtccatcg
    22381 gacaaccgct ttcctgccag cttcagttag ccgggccgct agatcggcca aggcgcaaac
    22441 ctggaatacg tcggcttgct tgtccgcaag caatcgcttc agggcggcca tctgaaacag
    22501 gcggtcatcc attctccgcg cgcggagaga ggcagcccgc aagattctcc atggactccc
    22561 gctgtatcga tactccacat ctcgcaggta cggactgcga atgtactcag tctcgaactc
    22621 atccaacggc tggtcaacac ttgcgacctt gcgccccacc acaaagcgaa cgtcgactcc
    22681 gagacttctc agtgctcggg ccatgttcaa atcaaaaagc tcccccccgc cgcggagtat
    22741 ccccatcatt ctattgacaa acgtgattcg cacttcaacc ggccgctgca gagcgggtca
    22801 tggcctgttc acctcggcca gacgtcccag tgggaggaac tcgatacggt cgacgtgctg
    22861 ctgcgcctcc tcccacaact gttccaccat ctctcggaca gtgacagacc cgcatcggct
    22921 gggggcatcg aattcccagt agtgcgtcgc caagcagaac acgccgccag tcttcagcac
    22981 gctcctgaag gcacgcatca catggtcaag gccaacggat ggactgactg tcaaatgggg
    23041 cacctcgcga tgtccatccg gaaagacgag cggccatggc cactcgcacc catgctggcc
    23101 gtaccaccac cgccgcttga gcccgagcca tgcgatccgc ggatcccatc ctcgaaagga
    23161 cgggtggaat gactggatac cgctgatgtg caggccggcc ctcacgactg ccctgtaacc
    23221 ttcccagctg agcgcgttgt gtggcggaac gaacacggtg acaggctgtc ccagaaggct
    23281 ttctagatac tgtctaccgt cgcggacctt gcgatcaaga tctgcgccgg caacgaactc
    23341 gaatcctcgc acctcgtcgc gatgatggta accatgcagg gtcacatgaa tacgtccatg
    23401 cgacatgttc tcccgaagaa aggccaccag ttctccattc gaagccagtg ggaacatcct
    23461 gtctccgcgc cagtattcac gcggcaaggc tccagacttt gtacaaccgt ggaatggaac
    23521 aactgataag gatatgggac agatcctcca aatgcgcttg tagcaacgct ccaggtgttc
    23581 cggccgcgtg aaatacgacg tatcgtcgtc ccgcaacgag aagaagatgc gatcagtcat
    23641 catggcaacc gcttatcggc atcttagtcc atgaacctgc gcatccacag ctcaagcatc
    23701 gtcatgcgac cgaggaaacc ggcaagtcgt ttctcacctt ccgcgtatcg gcgcacaagt
    23761 ttggacactg cctgactctg gacgaagtca cgtgatcttg cttgcgaaga cagcaatacc
    23821 gtatcgaaca gattcgcgag ccgccgctcg atccaccgct ccggaacatc cgcccgacca
    23881 taacccaatc gccgggctat ccggtaggca tacgagacga gttgcctcct caacgggctg
    23941 gccgtcaatg gtactctcat cgccgagttc gggatggtca acaatccggc atgattcctg
    24001 gagacgagca agttctgcat ggtagtcccc agccgcagcc tgaggggaga acgcagaatc
    24061 gcacaaatca ctttcgggtc gagaaagggt agcgagacag ccagttcggt gcggagacac
    24121 ctcatgcttg acatcatccg ccgcgaaagg aactcggtga tgaatagaat cgaaacgcat
    24181 tggccggggt agacagatcc ctcccacttc gcatacgacg cgcagaacgt gcttcgcggt
    24241 ccatcgagca tgacatctcg ccagtcggcc gccgaaatag cacacaaatc gacgccctcg
    24301 caggaagcga tgagttgcct gtacagatgg tctcggacag cgacatgatc ggagccaaga
    24361 gccaattcag caggaaccgc gaaggaatag gctcgttcga gtttggttag ttcaccacca
    24421 tgacctcgga tcacatggga aatgcccaat tcgtggcggt acttgcgagc cacgtacaac
    24481 atcataatat ccaagagact tgcttcgcca tcgctcgcct ccacgacgcc tgagagccaa
    24541 tcagaagcct tctccacaag gtctggaccg aagaggcatt tgatgtgttt gtgtccaact
    24601 gaccgacaca tccggctcgt caactcgagt tcgatgcctc cctgcacgcc atttgtcaca
    24661 caagcgagat ccaggccctc cctgtgagtg attgccaaaa gcactcgcga atcaaggcct
    24721 gcggacaggc aaattgctgg cacgtcacca gcagccgcag cgcctctgca cgccgttcta
    24781 agcgagtcgt caatctgatc aatagcagca gcgggattgg gctcttcgtc gccgcttggc
    24841 aacacgaagt atctcctgac ctcagatttg cctgcttctg gctgaaaatg caaagcgcat
    24901 ccggcttcaa gcaatgcaat acccttaaca agcgtcttgt tacccatcag gaagccgaac
    24961 aacaaatact gcgcagcgcc ctcccgatca agatggcggc cgaccactgg gccatccagc
    25021 agaagatgag cttgagagga gaaagcaata ccgtgatcaa ggagggcata gtagagcgga
    25081 aactgtccaa cgtgatcccg ggctagaacg accttgcgcg atctaatgtc gccaacaaca
    25141 gcaagaaaag ccccgtcgag gtcagcaaat atgccatccc cttcatcttc atagcgttcg
    25201 gcaagcctcg cagcgaaatc tccaaccgca tttgcctggg agccttgccg gttgcggata
    25261 gcccaggctc ctgttgtcac gacgccggca atcgtcaggc ccgattggga ttcagccgcg
    25321 ccaacgcaga ccccggacgc gagcccatcc ccgagaacgc acgtgagccc gagcaactct
    25381 tgatttaaat gtatcccggg tctggtgctt gccccagagg ccaattgcac gattgtgagc
    25441 cactgatgca ggccgatttg accccgcgat tgacaagtac caagcacaaa catcaggtca
    25501 gcagcctctc gcccccagcg aatgtaaaga gcacgatgag ttctagcaat tcgttatagg
    25561 taagagaatc ggagttcctg gctaggtcgt gttgacattc aggtcatcca acaaaagagg
    25621 gcggcgagtt ggacgaagct catgaagttc cgggcggttt tctcgtagcg ggtggcgacg
    25681 cgacgaaaac gcttgatccg attcacgtaa ctctttagcc ccatgcaaca ccgttcacag
    25741 gattgctgtg tgttttgggt gaacatggcc gatacgaatt atcatgcaag caacggcgga
    25801 gccgatgcgg gtggaagggc tgggcgcggg cctgacggag gaacaggcgc gggagatcta
    25861 tcgccaaggc gaggaggcgg ttgtctttgc cttgttggag caggccaagc tgctggccga
    25921 agcccaggcc aacggttccg ctccacccca cccgatgtcc cccagcacgc cctcggggat
    25981 gaggccggtc tacaccaagc cacccgggtc gcgacgccag aagcgtccgg gctgcaagaa
    26041 gggtcatccc ggcgcgcggc gcagccgtcc cgaccggatc gatcagcggc gggaacaccg
    26101 tctggagtgc tgtccgcact gtcaggggcc gcttcatcgc tgccatcaaa cccgcacccg
    26161 ttacaccgag gacatccccg aaaacattca acccgtggtc accgagcaca tcatccatcg
    26221 cgactggtgc ccacggtgca agaagcacgt ggagccggtg gtcaccgagg ccctgcccgg
    26281 ctcgacgctg ggccatcgcg tgctggtctt gtcggcctgg ctgcactacg cgctgggcaa
    26341 cacgctctcg cagatcgtcg aggtgttcgg ccatcatctc caactggaac tgacgccggg
    26401 cggcctggtg cagatgtggc accggctggc cgagatcctg ggcggctggt acgaacagat
    26461 ccgcgacgag gccttgcgag ccggggtctt gcacggggac gagaccggct ggcgggtcaa
    26521 tggaaagaca cgatggctgt ggtgcttttc gacggtcgac ctgacctttt acatgatcga
    26581 tcgttgccgg ggcagtccgg ccctgctgag gttcttccaa caggcattcg gggggacgct
    26641 ggtcagcgat ttctggggag cgtacaacgc ggtggcgtgc tccaaacggc agaagtgcct
    26701 ggtacatctg ctgcgcgatc tggaacacgt ggaaacctac cgccccgtga atcaggactg
    26761 gccggcgttc gccaagaagc tgcggcggct gatcggcgac gccatccgcc tgtggaagcg
    26821 ggaggcggtc ccccccgccg agtatgcgtc gcggcgggcc cgtctcgatc ggcgcttgca
    26881 ggaactgatc gagacgcggt ggacccatcc ggacgcccgc cgtttgatca aacgcctgcg
    26941 acgacatcac aacgagttgt tcacgttcct cgacgagcca ggcgtgccgt tcgacaacaa
    27001 ccatgccgaa cgcgccatac gcccggcggt catcattcgc aagaacagcc agagcaaccg
    27061 cagcgaccgc ggcgccgaga ctcaggccat tctgatgagc gtgtatcgga cgctgaagca
    27121 acgcggatat cctccaatcc gaaccatcgt cgatgccatc aggagctacc tgactaccgg
    27181 acaattgccg ccgctaccag cgaagatcgc tgcaagcggc taaagagtta ccgattcaca
    27241 aatcgctccg ccaggttgcg ttgccggtac tgcttcctca gcaca
//
LOCUS       DAOTPW010000043        27132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1854_length_27132_cov_5.891864, whole genome shotgun sequence.
ACCESSION   DAOTPW010000043 DAOTPW010000000
VERSION     DAOTPW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27132
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1854_length_27132_cov_5.891864"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="P5316_05830"
     CDS             <1..662
                     /locus_tag="P5316_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84513.1"
                     /translation="REQTQSLDRWIKAAPTAGDNLHEVYTMTTEAADRQREAAHTMLE
                     RIREQSQALDKRIAELKSTVAGAGEQIDRMTALTAEARTAAFDLSFNLAQVQQHAELA
                     EASCTSRMQDLTAKRAEAEDQVSQLEAVLRRAEKMATELPAQLEAFFEVQKQVGMTIE
                     ALEGAGASGEARSRLAEAAARSIRAVQAIREGAETRRENADRIPPSGAKEETASASTT
                     T"
     gene            complement(777..1391)
                     /locus_tag="P5316_05835"
     CDS             complement(777..1391)
                     /locus_tag="P5316_05835"
                     /inference="COORDINATES: protein motif:HMM:NF022389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HRR84514.1"
                     /translation="MDDVELGQTLQRVADAKFVLYRRRLHPELFRIHEIKHLERPAYS
                     AEIWVMGLSHAVTVQADSRCITEATTDDLEVLPQNGLVTSFQFRGERDHVEEFDDGMK
                     YILSTQVERMTQNLFHASHRDLMRYASTRGLLVRFTEWAQGDELVPFTFIDYEARDRE
                     LHVQSFHVFPADYTILKTQSIVEIGRVPVQRKPARPVNGHGRHN"
     gene            complement(2066..2632)
                     /locus_tag="P5316_05840"
     CDS             complement(2066..2632)
                     /locus_tag="P5316_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84515.1"
                     /translation="MPVASVMSPSSVAVTGPDKPVTSNTYGPLPLTDTTVPTERLRSS
                     AETLSTGSLRDTTIRRTTASVLPSVGQVFQTVGQKAVAGGLGGLVMPDLVVIGIDGNC
                     QPHRVARQSIVAKLDKPFRDRAVIACPAPHIERWFLADARAFTSVVGSSPKVRKGKCQ
                     RDYYKGVLLQAVVAGGHVPTLGGIEFAR"
     gene            complement(3132..3941)
                     /locus_tag="P5316_05845"
     CDS             complement(3132..3941)
                     /locus_tag="P5316_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84516.1"
                     /translation="MSWKTDKDSPILVSEVGGRQVCTVHAAEVPAERRSVVELSRPNE
                     SLTFVNSKGGVTSFALSSLFDEGRHLFLCIRIGSGLGVQCDCLISSSGEDPTEGFRQG
                     LAKGVRFQPFYLPESKGNPDELVGRGLFSRGFHFPGTITPVNVSLLCICDYCLRNFRL
                     QSFHAGFSDLTYLYCSQGPHTLVASSYLDDAPPVLGVADAASVSRFESRLPRCEKCGG
                     EFRHRNPLRCPHCGKPYIDFLRHPQERQFEYYGNHLYGDTLQEWSGDRTPS"
     gene            complement(4232..6328)
                     /locus_tag="P5316_05850"
     CDS             complement(4232..6328)
                     /locus_tag="P5316_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84517.1"
                     /translation="MIRPGLPLAGVLIVGLAVPTSLAAQDTELKVSRIALFSSGVGFF
                     EREAEIDGNAAAELKFRTDQINDILKSLVVQDLGGGTVNAVGYASRDPIEKTLKSFAV
                     NITGKPTMANLLDQLRGEPVEITGAQAIRGIIVGVEKQKIAIDKEIIEIDLLNVLSEL
                     GLQQLRVSDIRGIKLTNEKLEGELRKALETLAGSHEADKKGVTIQFAGQGKRLVRASY
                     LLEAPIWKTSYRLVLSPDKKPFLQGWATVENATEEDWNDVRLSLVSGRPISFTMDLYT
                     PLYVPRPKEELELYASLRPPTLEAGVPLAPGAAPAPARRAMAASKAPEVREETVAQAG
                     AARARTAGDAAGHYAAGALVAMRDADADGVLDLADHGVQSVAEAQSAGELFKYVINTP
                     VSIARQRSAMLPIVNQEVSAQKVSIFNPATHPKHPLNGFILENTTDLNLMQGPITVFD
                     ENVYAGDAKLPDLKPAEKRLLAYALDLSAEVIVDHKPQPDSIVSLRIAKGVLWHKHKH
                     VDTREYTIRNKDKKDREVIIEQPYTADWTLVEPKEPFERTQNILRFKTGVPAGQTVVQ
                     KVQLERVSDQTLALIDSDQDAVRFFLRSKVISPAVKQALEKVIALRSELYDLVRAREL
                     QESELKKAVEEQARIRENLKTLQQGSDPYQRQLKKFDDVETQIEKLKGQVSELRQTEE
                     SKRKELEAYILSLDVE"
     gene            complement(6471..8576)
                     /locus_tag="P5316_05855"
     CDS             complement(6471..8576)
                     /locus_tag="P5316_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84518.1"
                     /translation="MIRPIYPLVAVLVVVLAVPTLLTAQETELKISKIALFSSGVGYF
                     EREAEIDGSAAAELKFRTTQINDILKSLVIQDFGKGTISAVGYASHDPVDKALKSFAV
                     DITGKPTMATILGQLRGEPVELTGSKAVSGIIVGVEKLRIAREKETVEIDVLNVLTES
                     GLQQLKLSEIGGVKLKNEKVNGELRKALDTLAASHDADKKAVTIQLAGDGKRRVRASY
                     LLEAPIWKTSYRLVLSPGKKPFLQGWATVENATEEDWKDVRLSLVSGRPISFTMDLYT
                     PLYVPRPREELELYASLRPPTLEAGIAVADQPAKPAPAPAAEAPQAGKESRGRARSAM
                     APMRRVHAVGGMGGYGMGGMMGGMGAPAEVEPEMDLAAQGVESVAEAQSAGELFEYAI
                     NTPVSIARQRSAMLPIVNQEIAAEKVSIYNPATHRKHPLNGLIVDNTTGLNLMQGPIT
                     VFDENVYAGDAKLPDIKPAEKRMVVYALDLGVEVIADQKERPDELLSARIAKGTLILQ
                     HKLVDVSEYTIRNKDKKDRDIIIEQDYNNEWRLVEPKEPFERTHNLLRFRTLVPAGET
                     VVQKVQLEAVTDEEIHLTTIDEEFVGIYLKSKVISDAVRQAMETVIKIQSELEALERD
                     LDQREEDLERSVEEQARIRENLKTLQANSDPYQRQLKKFDLVETRIEQLQGQLDELRR
                     QVQTKEQELEAYVLSLDVK"
     gene            complement(8698..9900)
                     /locus_tag="P5316_05860"
     CDS             complement(8698..9900)
                     /locus_tag="P5316_05860"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR84519.1"
                     /translation="MSIRGSSATPFSAALLLSTLAFVAWNALGLPLSTAATSQPAVTV
                     VKATTTAPAETGVVRKALKRLVTIPRPGTELPDETVRPSKASPAPVVPQGKTSGAAPA
                     VKPVQRAILRAGQNSEQQPQTEQPPADQPQPAPEASYVYPVPAPYVYVPPPYVQMPLN
                     EMSPADREWNSYLSFGGRPWGYGYGYGYGPYYGGYSYYPGESWGDAYRFGFTSGYNYW
                     RFHKTSTERQESVAVHALNAMDRGLKFYREGKYRQAIDAFKLAAETDQGDPAARIYAA
                     HALFATGRYREAMPFLARAFELQPKLALLDYDMRGDYGRPADFEAQVRALESALAQSP
                     SSVERLIMLGYVRFYSGQKDAAYDPLSKAKILDPANALPDRLLPHCTPPDVVLDSQKT
                     AAKPATTR"
     gene            complement(10016..10675)
                     /locus_tag="P5316_05865"
     CDS             complement(10016..10675)
                     /locus_tag="P5316_05865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRR84520.1"
                     /translation="MYPFLKRAVDILVSLVLIVLFSPVILAAALAVKLSSPGPVFFVQ
                     PRGGRFGRPFMSIKFRTMRADHVHDVHEVVPLTHPNITRLGRFMRRFKIDELPQLFNI
                     LRGDMSLVGPRPTIMEQVVAYNDFQRRRLDVRPGLTGLAQVNATAAMSWEERIKYDVY
                     YVDHCGPLLDLLILAKTPVVVLLGEERFARPFEQSPYARKQRYSTQDNPAQNDSCNIR
                     S"
     gene            complement(10699..11130)
                     /locus_tag="P5316_05870"
     CDS             complement(10699..11130)
                     /locus_tag="P5316_05870"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRR84521.1"
                     /translation="MSGRLPDAEYAPGLLPDFQRQLREYFAGRRRKFDVKLDLSALTP
                     FQRQVLDRCAQIGYGRTKTYGQLAREVGRPKAARAIGGAMARNPLPLVIPCHRVVAGD
                     GSLGGFSAEQGVSLKRRLLDMEAGRPASRLLLPRRRRRPAG"
     gene            complement(11362..12081)
                     /locus_tag="P5316_05875"
     CDS             complement(11362..12081)
                     /locus_tag="P5316_05875"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR84522.1"
                     /translation="MNTLFPMEAAHQANVLADAIEDLRPALATWRQGDRWAAYETRFV
                     NVDYSAGELTLQYDASEQGVPELSDGQEVCVSFRRGRRRCAFDTVVLGRGRAPVGDAE
                     TVSTIRLKYPRELCELQRRTYYRQKVPANVVVPVSLSIASDGATTAADARGNLLDISP
                     DGMGVHVTEGSGMSFDVAFLADCTLHVQAGATPIRVRARVCSRTNTPDGLVRLGLQFV
                     DDDQLARARADLARLLASVRT"
     gene            complement(12078..12890)
                     /locus_tag="P5316_05880"
     CDS             complement(12078..12890)
                     /locus_tag="P5316_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84523.1"
                     /translation="MAYAIELSARQSSRTLEQAVRHHAEILIYPRLFEDGDPIVSRLV
                     SIETPIGWQSRRPCLVLETFSAGPVNRNDTADKGESEAAGLDSLVGTYCDVMLQLGEN
                     RYLFSSDVVAVQGASGTGARPRIYLARPETVQVAQRRRSWRFRPAQSSRVEMTWINAE
                     KSKTEGIGWLCNVSAEGLACRTEMAVAEQLGIGEQVCLSFALTPGDPERFVIDAVLCN
                     KTPAGTEGTMILGLQFLVGQGHQASAAAAEALRRKLLARYAPVVDTPGGAAQ"
     gene            13290..13976
                     /locus_tag="P5316_05885"
     CDS             13290..13976
                     /locus_tag="P5316_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84524.1"
                     /translation="MADAQSGLPTTDLEKTGTWKKALARTAVYKRVPFSTRRALDRAI
                     LARPEGCATLEAIEARFRLTERFGVSRDNLRTYARKLERVAEPCFAGNLAAAILGCMP
                     TAYGSQVAAGSRIILVSRLVQALTQGESRLEIPELIKLASALRLAHATAVARNAAMKG
                     CGGRGKDRGRDRKDSWCFSNQAALAEAVRTVYGLEYPAHRPTGAETPGSPKPEIARQP
                     TRRYDEKPTD"
     gene            14001..15137
                     /locus_tag="P5316_05890"
     CDS             14001..15137
                     /locus_tag="P5316_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84525.1"
                     /translation="MSNLPELSSRIPFYSGQVVYIYSFDIAYEFLRQPEGNLLGQPIV
                     QVSVGPSKRSPRQLVFYRPNMINLPPEERVGPHGPVTVERAVKLLSVGAISITVRVPF
                     KVSRIEQLVDYHDLQFADGSLTEGVQELAEQVRKELIPYCDRPVSQVGEGEAYTVFCI
                     TSPLVTEEGSLLSAEAWLEDRRRQLASILTQEEDMSLLSEQEVEETTSRYCSYYGHDL
                     TMVDWDAAIIINDPQDMEETLYIMELANVQLTELEAYDRLLDEAVMRSYRDLESARLR
                     GRSPVMAALREIRIDLARLSDELSNITKFFGDWHLARIYQMLSERFHLGDWHRVVGGK
                     LNTLDSLYSLHKQDQTNRWMLVLEATIVLLFVLDLIVLVMGLGK"
     gene            15333..17051
                     /locus_tag="P5316_05895"
     CDS             15333..17051
                     /locus_tag="P5316_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84526.1"
                     /translation="MSRTVALALLLFHSCLRTSPTVDGQCLNWSPPTNVSRDVGHYAV
                     DGNPAIDAFGKVHMVYQSFLDDYGETFYVTDLSGDWSSPQSLGSQGGKGSAPKIVITP
                     DDMLHTFYGKNKLYWRTKPVSGGQWSSPAQIDVNPGGGSFIQQVTVDGSGGIYFMYGH
                     LFDSSAPVRNGIYGRYKPLGGAWNTTELIYGNNDDGNWPRGDDIAARGNMLWIAIGVD
                     GDAYFKKKPSTGVWPAGKGTKLVDDAGGMRFAFSPMSGEIVAFYTKSLPCTDPCEDDP
                     WFEVFARFSHDDGATWSAPQSISDNVDDIDRTPSAVYDSAGNLHVVWESFCCDHKLRM
                     RYRGRMNGQWDPTITRITSHVGGHVPNSIKSFGSNLFLTFSDSGTGIGMYDVVFSTAA
                     VAEPKLALSPSSLTRTILVGSGVADDLLAVMNVCVGSLDYTVSVNAPWLEVSPPAGSV
                     TTETDAIVVSYPQAPAMTAGIYNATITVSGNAVNTPQTVAVQLKIQSVKPDFDGDGDV
                     DQTDFGHLQECMSGFAVPQTDPACSNTFLNPGDDFVDATELGIFLGCISGSGVRADVT
                     CAALHP"
     gene            17259..18395
                     /locus_tag="P5316_05900"
     CDS             17259..18395
                     /locus_tag="P5316_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006635482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR84527.1"
                     /translation="MQHRRFGKTEMNLSVLTLGLMRYMSKDLDQSARVVRRAVELGIN
                     HLETARGYDDSEELLGHALKSIDRGKVHITTKVGPCKTYDEFMNKFETSMSKMGIDVL
                     DNLDVHGINNEDKFRMAMDENGTLKACRKLLDSGAVRHIGFSTHGKTELVLKTINTGF
                     FESINLHYYWFYQAHAPVIARAAELDMGVLIISPNEKGGMLFKPPEVLTRISAPLHPM
                     NLNQRWLLSDPRIHTLSLGPAVAEELDAHMAVADDVGPLTEQEKAALARWDRTIREKL
                     RGDFCTQCGRCLPCPEFIDIPEILRLRNLAVGLEMNAYGSFRYNLLDGSNDWFHGWPG
                     SRCTKCGQCLPRCPEKLDIPRLLFDAHKRLKTGIGRRLWQRLVR"
     gene            19071..19559
                     /locus_tag="P5316_05905"
     CDS             19071..19559
                     /locus_tag="P5316_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HRR84528.1"
                     /translation="MVKSQPTAVYTFKVALKYSPDIWRLIEVPGNQTLEDLHFAIFEA
                     FERYDEHLYSFYFEPPGGYRGRRRPSRNAVEFTHPYAAEEADPWDGPAPHDASRTTID
                     SLNLHRGRKFEYLFDFGDSWWHEITVVKTDGHRRRGRYPRIIEKHGKSPPQYPDYDEG
                     IC"
     gene            19895..21037
                     /locus_tag="P5316_05910"
     CDS             19895..21037
                     /locus_tag="P5316_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRR84529.1"
                     /translation="MRDPRIDKLAQVLVNYSVAVKRDDVVRIVGSITAIPLIRAIYRE
                     VVIAGGHPVLRVTDDECTDIFFEHARPRQLAYSNPLNIHEIKNVDCSISIWANDNTRS
                     MTRVPPEKQAAVSKARRQFMDVFMKRAAMKGKGKLRWSGTQFPCHSSAQDAEMSLSQY
                     EDFVYGAGLLACKDPPAEWRKVSARQRRVCDVLNKAREVRFKTLQGTDIRFGVKGRRW
                     VNCDGHENFPDGEVFTGPIEDSAEGEVKFSFPAVYRGQEVLDVRLRLRAGKVVEASAS
                     KGEPFLIQMLDQDKGARFVGELALGTNYSIRQFTRNTLFDEKIGGTFHMALGAGYPET
                     GSRNKSALHWDMVCDLRQGGVVEADGKVISKNGRFLRADWPGPAGR"
     gene            21063..22046
                     /locus_tag="P5316_05915"
     CDS             21063..22046
                     /locus_tag="P5316_05915"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRR84530.1"
                     /translation="MKRVVSGILIAAVHAAMVLGVAPAARAAATSRPAETHSQMETRR
                     VSFTTDDGIEIHGTFVPSRAQAEDMAPIAILLHMYNTDHKDFLPLLPFLHMAGFAALA
                     IDVRGHGQSVGPAAMNLPKRVGDRDPKLFREMYRDVEAAYLWLADQPGVDTGRFVLVG
                     ASVGGSVVMNYAGRDRSVDAVVWLTPGTKYLGIDSMRDARKYGRRPLLMLAAEPERSA
                     AEALAAIVPGANVQLFPGQAGDQMALHGTRMLGQVAGIEKTIVDFLTKSVGPPATQPV
                     VASLSSDVYHTPDSPSVKRIKKANRRQFSSPTEAETRGLKPSKTAARKPAR"
     gene            complement(22097..22237)
                     /gene="rpmH"
                     /locus_tag="P5316_05920"
     CDS             complement(22097..22237)
                     /gene="rpmH"
                     /locus_tag="P5316_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006344410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRR84531.1"
                     /translation="MHYPRKISRRKKIRKQGFRARMRTSKGRKLINRQRRRGRHTLSV
                     MR"
     gene            complement(22391..23131)
                     /locus_tag="P5316_05925"
     CDS             complement(22391..23131)
                     /locus_tag="P5316_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84532.1"
                     /translation="MADSPTPAEPSFVGPKPIEALVIRPWPKIILMLPTLFAALICGI
                     LMLWYQMPTAEANATFGAVQHYIGLFFLVVLAVNLTMLLYDLSLKGFILVTLLIAVLI
                     LVLALINRSAETSIWAIIGRFLSVRVVANDRFYFLFAMVLLFNLGIAWIITRFNYWKV
                     EHNEIIIHRGFMHEQERHPTAQARFKLVIEDVVEYAILGTGKLVFYFGDSNTQHELST
                     VLFVHRKAKKLDELLGRLAVTQTAPTES"
     gene            complement(23356..24090)
                     /locus_tag="P5316_05930"
     CDS             complement(23356..24090)
                     /locus_tag="P5316_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84533.1"
                     /translation="MEERLEKLSWTGNDMPATAAAISPTAMALFAASMARGSSRITNA
                     AFDPAIPTLIDGLRQLGAMILADEATGCIEITGCGGYWVHGDLELNCWGQSPTAHLLL
                     AACVSGRSRYCLTNPPSMTASLASLINALTDLGAQIHPDHDASGSGIQIGPALFRGGR
                     ATVQNNDAPGAVASLLLVAPRAAGDVFLEVPLWPVRPPEVIVALQTLEDFGITVVDDQ
                     AARMIVPAPQCYDACQHDISIRRNVS"
     gene            24345..25355
                     /locus_tag="P5316_05935"
     CDS             24345..25355
                     /locus_tag="P5316_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HRR84534.1"
                     /translation="MTHTVCLVPGDGIGPEVTSVAKRVIEAANVDIEWVNLPAGETGI
                     EFFGNPLPERTIAAIIGHGIALKGPITTQVASGHGNVSIRLRQRLGLSAAVRPVKSIP
                     GVKALHPKVDLIIIRENTEGLFAGIENEVAPGVTQALKVCTADVSTRIARFAFEYALS
                     HDRRKVTVFHRADLLRRTDGLLLRCARKAHRSFSDQIVYEEMNIDNGCMQLVRNPSQF
                     DVILLDSLTGGMVSSIAAGLVGGPGMVPSASFGSQCVLFEPLHGSAPDIARKGIANPL
                     ACILSGAMMLEHLGEDAAASRIRSACELALSTGDASVLTPDLGGSGSTASLTDAVIRA
                     LK"
     gene            25352..26380
                     /gene="hemC"
                     /locus_tag="P5316_05940"
     CDS             25352..26380
                     /gene="hemC"
                     /locus_tag="P5316_05940"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRR84535.1"
                     /translation="MSKSRRQNELVVGTRGSQLARAQTNWVIERLRAAHAGLEVRAEI
                     ITTTGDRFQDRPLPEIGGKGLFTEELERALLDGSIDLAVHSAKDLPTDLADGLDVLAY
                     PAREDPRDAWVSADGAPFDKLPPESVVGTTSLRRQAQLLHLRPDLRFVGLRGNVDTRI
                     RKIHRGDCAGAVLAMAGLKRAGLEEHVTHPFDPQVCLPAPGQGALALEGRKGDERVVS
                     LLAVVHDEATAAAVRSERAVLSMLDAGCRAPVAILAEVIAEPMSVSGPTSVSGSPSPL
                     AKGGPRGILRCQALVLDPKGTRHVRAKAERPASQSDDLVADIVSQLRSGGGDEIIAKC
                     RFDEGTRR"
     gene            complement(26536..>27132)
                     /locus_tag="P5316_05945"
     CDS             complement(26536..>27132)
                     /locus_tag="P5316_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84536.1"
                     /translation="DLLLYWLSQWVEALPPFLLLIFLTTGLRRRSLARRPDEAPPLLS
                     RTRFNPRKDWLLLLVVIFINMGLLEAWIAAGFGGILRYLRLQGYPSGKAFCVLAIFIT
                     AGFAIWLWLSAILQWRKPRLVRTLAVLNLAIFTAGLMIQQMVNQLLWPSPSPLAVLAW
                     YAGRALLAGLLPLLPMLIFLFRDLTHSDLQLVSSPKQR"
BASE COUNT         5281 a         8169 c         8517 g         5165 t
ORIGIN      
        1 tccgggagca gacacagtcg ctcgatcgtt ggatcaaggc ggctccgacc gcgggtgaca
       61 atctgcacga ggtctacacg atgacgaccg aggcggccga ccggcaacgt gaagccgccc
      121 acacgatgct ggagcgcatc cgcgaacagt cacaggcatt ggacaagaga atcgcagagc
      181 tgaagtcgac cgttgcgggg gccggagaac agatcgaccg gatgacggcg ctcacggccg
      241 aggcccgtac tgcggccttc gatctatcct tcaacctggc acaggtccag cagcatgcgg
      301 aactggcgga agcgagctgc accagccgga tgcaggacct gaccgccaag cgggctgaag
      361 ccgaggacca ggtttcgcag ttggaagcgg tccttcgccg agccgagaag atggcgacgg
      421 agttgcccgc gcagttggag gctttcttcg aggtccagaa gcaggttggg atgaccatcg
      481 aagcccttga gggggccggc gcttcgggcg aagcccgcag caggctggcg gaagcggccg
      541 cccgctccat ccgggccgtt caggcaatcc gggaaggtgc cgagacgcgt cgagaaaacg
      601 cggaccgaat accgcccagc ggcgcaaagg aagaaacagc ctccgcttca accactacct
      661 gaatcccttt ccccacgaat ccataagcga ggcgcgtgga cgagtctcat cgaacaaaaa
      721 gagggcggag gggtcttgag acccgtccgc cctgaaaacg atgcggggag tctactttag
      781 ttgtgccggc cgtggccgtt gaccggtctt gcgggcttgc gctgaaccgg gacgcgaccg
      841 atttccacga tcgactgcgt cttgagaatc gtgtagtccg ccgggaacac atggaaggac
      901 tgcacgtgaa gctcgcggtc gcgagcttca taatcgatga aggtaaacgg cacaagttca
      961 tcgccctggg cccactctgt gaagcggacg agcaaccctc gggtgctggc gtacctcatc
     1021 agatcacggt gcgaggcgtg gaagagattt tgtgtcatac gctcgacctg ggtcgagaga
     1081 atgtatttca tgccgtcgtc gaactcctcg acgtggtcgc gttcgccgcg gaattgaaac
     1141 gacgtcacaa gtccgttctg ggggaggact tccagatcgt cggtcgtcgc ctcggtaatg
     1201 caccgggagt cggcttgtac ggtgacggca tgggaaagcc ccatcaccca gatttccgcc
     1261 gagtaagcgg gccgctccaa gtgcttgatt tcgtgaatgc gaaacagctc cggatgaagc
     1321 cggcggcggt acagcacgaa tttcgcgtcc gccactcgct gcaatgtttg gcccagttcc
     1381 acgtcatcca ttccttcgag acgctcctgt ctcaattcgc gccgcaactc tgtgcggctg
     1441 atctccaatt caccaccctg gacagacgca acgcacgccg tgccatctct aaaagggatg
     1501 gtgagtgccg cctctagctc tcctcaagct acccaccgcg gcggctactc cctcctcgta
     1561 ggcgggggtg ggcttttcgg tcgaggccca ttataacctc tcttgaagcc cgagccaaga
     1621 gaaaaatcgg caaaacgtca tttttgccct ctctgccacc ccggcgggct atctatatgc
     1681 tatgtacgaa gttagcatcg catggttgcg gcatgtttcg ggtttatttg agatttgcgg
     1741 gcttggtcac cgattgacaa atgacgattt tccgtctgac atctgagtaa caaatgttac
     1801 cagggatgaa atcaaagcgg aagcgggtaa cgactctttt ggagaagacg ggagcaccga
     1861 ggccggggct cacggcctaa ggcggaccgc ctttttgcgg gccgcaaccc aagtgctctg
     1921 cgatccggaa acatgcgccc aaaccgcgct ggtctctggc cataaggttc tatagccttt
     1981 ccagagaggc accgatctcg tcgagaaacg ccttcaggct cctgtcggtc ttgccggcgt
     2041 ggtacaaatc cgtggcctcg acgatctatc gcgcgaactc gatcccaccc agcgttggta
     2101 catgcccgcc ggcgacgacg gcctgcagca gaacaccttt gtagtaatcc cgctgacact
     2161 tgcccttgcg gaccttcggg gacgagccca caacgctcgt aaaggcacga gcatccgcca
     2221 gaaaccatcg ctcgatgtgc ggggcggggc acgcgattac ggccctgtct cgaaacggct
     2281 tatccagctt cgctacgatg ctctgtcgag ccacgcggtg cggctggcag ttcccatcaa
     2341 tgccgatgac taccaggtcc ggcataacca gaccaccaag tccccccgcg acagccttct
     2401 gaccgactgt ctggaatacc tggccgaccg acggaagtac cgaggcggtc gttcgccgga
     2461 ttgtcgtgtc tctcagcgat ccggtactca acgtctcggc tgacgacctg agtctttccg
     2521 tcggcacggt ggtgtccgtc agcggcaacg ggccgtacgt gttcgaggtg acgggcttgt
     2581 cagggccggt gaccgccacg ctggacggcg acatcaccga cgcgacgggc atcgatctgc
     2641 ccgcgtatca atggcagttc gccatctgca cgcccgacag cgacggcgat ggcgtcggcg
     2701 actgcggcga tgcatgtccc aacaccatcc cgggcgcaag cgttgacctg cggggctgcc
     2761 cgcacggcga ttcatcatcg agcccccacc cagcgaagaa tgtgtgtgaa gacggtaggg
     2821 cacggtcgga gccacccaag gtggcgcaga tctgtcggtc ctggctgcag aatatggcag
     2881 atctcggtcg ccacggcgac ctcggcactg ccctacccac cggccgcgtc ggcttgccta
     2941 catattgcac ggtgccgatc gtcgctcccc caactgcaag aagcaggtgg ggcacccggc
     3001 cgatcggcgt gatttgcgtg agctgcgggc cacccccacg cgaagagttc cgtcctccac
     3061 cgccgcgcca tggggcgccc cgcgtcgccg cgtcccaacc cagttcatcc gtggagaagt
     3121 gcttttcgac atcagctcgg cgtacggtca ccggaccatt cttgcagggt gtctccatag
     3181 aggtggttgc cgtaatactc aaactgcctt tcctgggggt gccgaaggaa atcgatgtac
     3241 ggcttgccgc aatgcggaca gcgcagcgga tttcgatgac ggaattcccc accgcatttc
     3301 tcgcatcgag gaagccggct ctcaaagcga ctcacagaag ccgcatcggc caccccaagt
     3361 acgggcggcg catcatcgag ataactgctg gcgactaagg tatgtggccc ctgcgaacag
     3421 tacaggtaag tcaggtcgct gaaaccggcg tggaaggatt ggaggcggaa gttccggaga
     3481 cagtagtcgc atatgcacag cagcgagacg tttacgggcg taatcgtgcc gggaaagtga
     3541 aagccgcgag agaagaggcc acggccgacc agctcatcgg ggttaccctt ggattccggc
     3601 agatagaagg gctgaaaccg cacccctttt gctaggccct gtctgaagcc ttcagtcgga
     3661 tcctccccgc tactggagat gagacaatcg cattggaccc caagaccgga tccgattcga
     3721 atgcacagaa agagatgtct accttcatcg aacagcgacg acaaagcaaa agacgtgacg
     3781 ccccctttgc tgttgacgaa agtcagactc tcgttcggac ggctcagctc aacaacggat
     3841 cgtctctctg ccggcacctc ggcggcgtgg acggtgcaca cttgcctccc gccaacctcc
     3901 gaaacgagta tcggactgtc cttgtcggtc ttccagctca tgcacactcc cggcaggtat
     3961 tcgcgggcgc ccagggactg acagttcttc gaccagccgt cccacgaaat cggtatgccg
     4021 tcgcgctcga caacaccaac gtctttgcct cctctcgggt cgcgcgatcg tacttgaact
     4081 tcgaccttct gtcatgatgc tgctggccct gcacgagaca aagatgtcgc ggcagcggca
     4141 aaaccggcga gaaaggaggc aacgaatcac aaaccacgcc cgcgaggtgt agtttgtgac
     4201 ttcctggtgt tgcgggctgc aacgctgggg ctcactccac gtcgagtgac aggatgtagg
     4261 cctccaattc ctttcgcttg ctctcctcag tctggcgcag ctcggaaacc tggcctttga
     4321 gcttctcgat ctgcgtttcg acgtcgtcga acttcttgag ctgccgctgg tacgggtcgc
     4381 taccctgctg gagcgtcttg agattctcgc ggatgcgggc ctgctcttcg accgccttct
     4441 tcagctcact ctcctgcagc tcgcgcgcgc ggaccaggtc atacagctcg ctgcgcagag
     4501 cgatcacctt ttcgagggcc tgcttgacgg ccggactgat caccttgctg cggaggaaga
     4561 atcgaaccgc atcctgatcg ctgtcgatca gggccagcgt ctggtcggag actcgttcaa
     4621 gctggacctt ctggacgacc gtctgtcctg caggaacgcc cgtcttgaac cgcaggatgt
     4681 tctgggttcg ctcgaagggc tccttgggct cgaccagcgt ccagtcggcg gtgtagggct
     4741 gctcgatgat cacctcgcgg tctttcttgt ccttattgcg gatggtgtac tcgcgggtgt
     4801 ccacgtgctt gtgcttgtgc cacaggacgc ctttggcgat ccgcagactg acgatgctgt
     4861 cgggctgggg cttgtggtcg acgatgacct cggccgacag gtccagggcg taagcaagga
     4921 gccgcttctc ggcaggcttg aggtccggaa gcttcgcgtc gccggcgtag acgttctcat
     4981 caaaaacggt gatcgggcct tgcatgaggt tcaggtcggt cgtgttttcg aggatgaaac
     5041 cgttgagcgg gtgcttgggg tgggtcgcgg ggttgaagat gctcaccttc tgggccgaaa
     5101 cctcctggtt gacgatcggc agcatggccg accgctggcg ggcgatgctg acaggagtat
     5161 tgatcacgta tttgaacagt tcgccggccg actgtgcttc ggccaccgac tgcacgccgt
     5221 ggtcggccag atcgagcacg ccatccgcat ccgcgtccct catcgccacg agtgcgccgg
     5281 cagcataatg gcccgccgca tcaccggccg ttcttgctct ggcggctcct gcctgagcta
     5341 cggtttcctc acggacctct ggtgccttgc tggctgccat ggcccgtcgg gccggggctg
     5401 gagcagcacc tggtgccagc ggaacaccgg cctcaagggt cggcgggcga agcgaggcat
     5461 agagttccag ttcctccttc ggccggggca cgtacagcgg ggtgtagaga tccatggtga
     5521 aggagatcgg ccggcccgag accagcgaga ggcggacgtc gttccaatct tcctcggtgg
     5581 cgttttcgac cgtggcccag ccctgcagga acggcttctt gtctggcgag agcaccagcc
     5641 gatagctggt cttccagatc ggggcctcga gcagatacga ggcccgcaca aggcgcttgc
     5701 cctggccggc gaactggatg gtcacgccct tcttgtctgc ttcatggctg ccagccaggg
     5761 tttccaaggc cttgcgcagc tcgccttcca gtttctcgtt ggtcagctta attccccgga
     5821 tatccgagac ccgcaactgc tgcaaaccga gttcgctcaa cacgttgagc aggtcgattt
     5881 cgatgatttc tttgtcgatg gcgatcttct gcttctcgac gccgacgatg atgccgcgaa
     5941 tcgcctgggc cccggtgatc tcgaccggtt cgccgcgaag ctgatcgagc aggttggcca
     6001 tcgtgggctt gccggtgatg ttgacggcaa agctcttgag ggttttctcg atcgggtcgc
     6061 gagacgcgta gccgacggcg ttgaccgtgc cgccgcccag gtcctgaacg accagcgact
     6121 tgaggatgtc gttgatctgg tcggttcgga acttgagctc ggcggcggcg ttaccgtcga
     6181 tctcggcctc ccgttcgaag aaccccaccc cgctggagaa cagggcaatt ctcgacacct
     6241 tcaactcggt atcctgggca gccaacgacg tcggaaccgc caaaccaaca ataagcaccc
     6301 ctgccaacgg caaacccggt cgaatcatga catggctcct ttcagatgct gcggaagcac
     6361 tgtattcaga ctcaattcaa tggttagacg cccgaagccg cccggagttc ccaaaaccgg
     6421 gccgtttctc cgaaaaatcg cctcgccacc cggcggccgg accgccgggc tcacttcaca
     6481 tccagagaca acacgtaggc ctccagctcc tgttcctttg tctgcacctg ccggcgcagc
     6541 tcgtcaagct gcccctgcaa ctgctcgatg cgggtctcca ccaagtcgaa cttcttgagc
     6601 tgccgctgat acggatcgct gttggcttgc agggtcttga ggttttctcg gatgcgggcc
     6661 tgctcttcca cggaacgctc aaggtcttcc tcgcgctggt cgaggtcgcg ctccagggcc
     6721 tccagttcgg attggatctt gatcactgtt tccatagctt gccgtacggc gtccgagatc
     6781 accttgctct tcaggtagat cccaacgaac tcctcgtcaa tcgtggtcag gtggatctct
     6841 tcatctgtga ccgcttcgag ctgcactttc tgcacgaccg tctcgcctgc ggggacgaga
     6901 gtcctgaaac gaagcagatt gtgcgtccgc tcgaatggct ccttgggttc caccagtctc
     6961 cactcgttgt tgtagtcctg ctcgataatg atgtcccggt ctttcttgtc cttgttgcgg
     7021 atggtgtact ccgagacatc caccagcttg tgttgaagaa tcagtgtgcc tttggcgata
     7081 cgagcgctca gcagttcgtc gggacgctcc ttctggtccg cgatcacctc gaccccgaga
     7141 tcaagagcgt agacgaccat gcgtttttcc gccggcttga tatccggcag cttggcgtcg
     7201 ccggcataga cgttctcatc gaaaaccgtg atcggccctt gcatcaggtt gagcccggtc
     7261 gtgttgtcca caatcaggcc gttcagcggg tgcttgcggt gcgtggcggg attgtagatg
     7321 ctcactttct cggccgcgat ctcctggttg acgatcggaa gcatcgccga ccgctgccgg
     7381 gcgattgaaa ccggcgtgtt gatcgcgtac tcgaacagct cgcccgcgga ctgggcctcc
     7441 gccaccgatt ccacaccctg cgcggccaga tccatctctg gttcgacctc cgccggcgcc
     7501 cccatgcctc ccatcatgcc tcccataccg tatccgccca tgccgcccac ggcatgcaca
     7561 cgacgcatcg gcgccattgc cgacctcgcc cggccacggg attccttacc tgcttgcgga
     7621 gcctcagccg ccggagcagg agccggcttc gcgggctggt cggccaccgc gatcccagcc
     7681 tcaagcgtcg gcgggcggag cgacgcatat agctccagct cttccctcgg ccggggcacg
     7741 tacagcggcg tgtacaggtc catggtgaac gagatcggcc ggcccgagac cagcgagagg
     7801 cgcacgtcct tccagtcttc ctctgtggcg ttttcgaccg tggcccagcc ctgcaggaac
     7861 ggcttcttgc cgggcgagag caccagccgg taactggtct tccagatcgg ggcttccagc
     7921 aggtatgacg cccgcacccg ccgcttgccg tcaccggcga gctggatggt caccgccttc
     7981 ttatcagcat cgtgactggc cgccagcgta tccagggctt tgcgcagctc gccgttcacc
     8041 ttctcgtttt tcagtttgac gccgccgatc tcgctgagtt tgagctgctg caggccgctt
     8101 tcggtcagca cgttcagcac atcgatctcg accgtctcct tctccctcgc gatcctcagt
     8161 ttctcgacgc ccacgatgat cccgctgacc gccttgctgc cagtgagctc caccggttcg
     8221 ccgcggagct ggcccagaat tgtcgccatc gtgggcttgc cggtgatatc aaccgcgaaa
     8281 ctcttcagcg ctttgtccac cgggtcatgc gaggcgtacc ccaccgcgct gatcgtgccc
     8341 ttaccgaagt cttggatcac cagcgacttg aggatgtcgt tgatctgggt ggtgcggaac
     8401 ttcagctcgg cggctgcgct gccgtcgatc tcggcctcgc gttcgaagta ccccaccccg
     8461 ctggagaaca gggcgatctt cgagatcttg agctcggttt cctgggcagt cagcaacgtc
     8521 ggaactgcca acacaacgac aagtaccgcc accaacggat aaatcggtcg aatcatggca
     8581 ccactcctct tctctggtga accagcgctt tcggatcagg cctgatcatg ggacgcacga
     8641 acccggttgg agacccctga cggggttcat cgtgagagtt taccctcggt tcgcagacta
     8701 ccgcgtggtc gcgggttttg ctgctgtctt ctggctgtcg agcaccacgt ccggcggggt
     8761 gcagtggggc aggagccgat cgggcagggc gttggccgga tcgagtatct tggccttgga
     8821 cagcggatcg taggcggcgt ccttctggcc ggagtagaac cggacatagc cgagcatgat
     8881 gagccgctcg acgctggagg gcgactgggc taaggccgac tcaagcgccc ggacctgggc
     8941 ttcgaaatcc gccggtcgcc cgtaatcccc gcgcatgtcg tagtccagta gtgcaagctt
     9001 gggctgcagt tcaaaagccc gagccaggaa gggcatggcc tcgcgatagc ggccggtggc
     9061 aaacagggca tgagcagcgt agatgcgagc ggccggatca ccctggtcgg tctccgcggc
     9121 aagcttgaag gcgtcgatcg cttggcggta tttaccttct ctatagaact tcagccccct
     9181 gtccatggcg ttgagggcgt gaacggccac cgactcctgg cgttcggtcg aggtcttatg
     9241 aaatcgccag tagttgtaac cgctggtgaa gccgaagcga tatgcatcgc cccaggactc
     9301 gccgggataa tagctgtaac caccgtagta aggtccgtat ccatagccgt atccgtatcc
     9361 ccagggtctg ccaccgaaac tgagataact gttccactcg cggtctgcgg gactcatctc
     9421 attgagcggc atctgcacat agggtggcgg cacgtaaacg tatggcgcgg gcaccgggta
     9481 gacatatgaa gcttccggtg cgggctgcgg ttgatccgcc ggcggttgtt cggtttgggg
     9541 ttgctgctcg ctgttttgtc cggctctgag aatggctctc tggacgggct tgaccgcagg
     9601 tgccgcgccg gacgttttgc cctgcggaac caccggcgcc ggggaagcct tcgacggtcg
     9661 gaccgtctca tccggcagtt cggtgcccgg tctgggaatg gtcaccagcc gcttgagggc
     9721 cttgcggaca actcccgtct cggccggggc ggtcgtcgtg gccttgacca ccgtcaccgc
     9781 cggctgcgac gtcgcggccg ttgacagggg caagcccagg gcattccagg cgacaaaggc
     9841 aagggttgac aacaacagtg cggcagaaaa gggcgtggca gagcttcccc ggatcgacat
     9901 ggcacgcctc caatgcttcg tgatgggtct gtgcggcacg tcctcctcat tatcaatacc
     9961 caccaaccaa atggcaagat gcggcccggt tggagccggt caggcctaaa ctgtttcagg
    10021 agcggatgtt acaagaatca ttctgcgcag gattgtcctg ggtactatat cgttgcttgc
    10081 gggcataggg actctgttca aaggggcggg caaatcgctc ctcccccagc agaaccacca
    10141 ccggtgtctt ggccagaatc agcagatcca ggagcgggcc gcaatgatcg acgtagtaga
    10201 cgtcgtactt gatgcgttcc tcccagctca tggcggcggt ggcgttgact tgggccagac
    10261 cggtcagccc cggacgcacg tcgaggcgtc gtcgctggaa gtcgttgtag gcgacaacct
    10321 gttccatgat cgtcggtcgg gggccgacga gggacatatc gccgcgaagg atattgaaca
    10381 actggggcaa ctcgtcgatt ttgaaccgac gcatgaagcg tcccagccgg gtgatgttgg
    10441 gatgagtcag gggcacgact tcgtggacgt catgcacatg gtcggcccgc atggtgcgga
    10501 acttgatcga catgaagggc cgaccgaagc ggcctccgcg gggttggacg aaaaacaccg
    10561 gtccgggcga tgacagcttg acggccagag cggcggccag gatcaccggt gaaaagagga
    10621 cgatcagtac gaggctgacc aggatatcga cagcgcgttt gagaaacggg tacatcaaca
    10681 catcctgaaa ggcccccctc aaccggcggg gcgtctgcgg cgccgaggca atagcaggcg
    10741 gcttgcaggc cggcctgcct ccatatcgag caatcggcgt ttcaggctga ccccctgctc
    10801 ggcggagaag ccgcccagcg aaccgtcgcc ggccaccacg cgatgacaag ggatgaccag
    10861 cgggagcggg tttctcgcca tcgctccgcc gatggccctg gctgccttgg gccgcccgac
    10921 ctcgcgagcc agttgaccat aggtcttcgt tcggccgtag ccgatctggg cgcagcgatc
    10981 gagcacctgc cgttgaaagg gcgtcagcgc cgacaggtcc agcttcacgt cgaacttcct
    11041 gcgccggccg gcgaagtact cccgcaactg ccgctgaaag tccgggagta agccgggggc
    11101 gtactctgca tccggcaacc ttccggacaa gcgttttcgg atttgcgagg gtcggcttcc
    11161 ggtaaggacg acattggata cgcctttgtc cgtggcgaca aaaccgaccg aacctcccgg
    11221 tatctcaacg actgcgtatt tcaacatatc agacaggtta gagagaaact gccgtggcga
    11281 caaggagcgc agccgttggc aagaggtcgc ttgggtgcgt tttgcggggc ttcgatgcat
    11341 cctgcgaaga agagattccg ttcacgtccg cacgctggcc agcaagcggg ccagatcggc
    11401 gcgtgcacgt gcgagttggt cgtcgtcgac aaactgcaag cccaagcgga ccaggccatc
    11461 aggcgtattc gtccgcgaac acacacgggc gcgaacgcgg atcggcgtcg caccagcctg
    11521 aacatgcagc gtgcagtcag cgaggaaggc cacatcgaac gacatccccg aaccttcggt
    11581 cacatggacg cccatgccgt caggagagat gtccagcaag ttgccgcggg catcggccgc
    11641 ggttgtcgcg ccgtccgagg cgatcgacag gcttactggg acgacgacgt tcgccggcac
    11701 cttctggcga tagtaggttc ttcgttggag ctcgcacaac tcgcgcggat acttcagacg
    11761 gatggtggag accgtctccg catcaccgac gggcgcgcgg ccccggccga ggacaacggt
    11821 gtcaaaagca cagcggcgac ggccgcgccg aaatgagacg cacacttcct gcccgtctga
    11881 gagttcgggc acgccctgct ccgaagcgtc atattgcagg gtcaactcgc cggcggagta
    11941 atcgacgttg acgaaacgag tctcatacgc cgcccaccgg tcaccctgac gccaagtggc
    12001 cagagcgggc ctgagatctt cgatggcatc ggccagcacg ttcgcctgat gtgcggcttc
    12061 catgggaaaa agcgtgttca ttgcgcggct cctcctgggg tgtcaaccac gggagcgtaa
    12121 cgggccagga gctttcgtcg caaggcctcg gctgccgctg ccgaagcctg atggccctgc
    12181 ccgacaagga attgcagacc gaggatcatg gtaccttccg tgcccgcggg agtcttattg
    12241 cacaaaacgg catcaatgac aaaccgctcg gggtcgcctg gggtgagcgc gaatgagagg
    12301 caaacctgtt ctccgatccc gagttgttcg gccacggcca tctcggtccg gcaggccaat
    12361 ccctccgcgc tgacgttgca caaccagccg atgccctcgg ttttgctttt ctccgcgttg
    12421 atccaggtca tctcgacacg ggacgattgg gcggggcgaa aacgccagga ccggcggcgc
    12481 tgggcaacct ggactgtttc cggtcgagcc agataaatgc ggggtcgggc gccggtgccg
    12541 gaggcccctt gtaccgcgac gacgtccgaa gagaaaagat aacggttttc gccgagttga
    12601 agcatcacgt cgcagtaggt gccgacaagg gaatcgaggc ctgccgcttc ggattcgcct
    12661 ttgtcggccg tgtcgttccg gttaacgggc ccggcactga acgtctcaag caccaggcaa
    12721 ggtcgtcgcg actgccaccc gatcggcgtc tcaatgctca ccagacgcga gacgatcgga
    12781 tccccgtctt cgaagaggcg cggataaatc aggatttccg cgtggtgacg gacagcctgc
    12841 tccagcgtgc ggctggactg cctcgcactc aactcaattg cgtaagccat gactgaaccc
    12901 ccggctccgc cagggctccc ctcgtgccga ccggcacagc agacacccgt gcccgccaga
    12961 cgcctcgcgg cagtttcggc ggcctgacac cctgggcatg tcgcccgttc cctctatcct
    13021 ctcattcggt gagcgcagat aacgacttaa ccttggggca ccgggcaaac gccggtcacg
    13081 gaatctgttc cgataagacg cgtcgggaga cttgttccgt caggatcgcc gtaagcggag
    13141 cagtcggagt gttgtacgct cgctgccgaa tgggggccga gaatggtccg ggaccgccgc
    13201 tgtcaaggcg aggacgttct gtcgaaaaac aaggagtcga tccgatgagg ggcaccatga
    13261 ttcaaaccga tcaaacccaa gcatcttgta tggcggatgc acagagtggc ttgccgacga
    13321 ccgatctgga gaagaccgga acatggaaga aggctctggc gcggaccgcc gtgtacaagc
    13381 gggtcccttt ctcgacgcgt cgggcgctgg accgggcgat cctggcgcgc cctgaagggt
    13441 gcgccacgct ggaggcgatc gaggcaagat tcaggctgac cgaaaggttc ggggtgagtc
    13501 gggacaacct ccgaacgtac gcccgcaaac ttgagagagt tgcggagccg tgcttcgccg
    13561 gcaaccttgc ggcggcgatc ctgggatgca tgccgaccgc gtacggcagt caagtggcgg
    13621 ccggcagccg gatcattctg gtcagccgtc ttgttcaagc tctgacgcag ggggaatcac
    13681 ggctcgagat tcccgagctg atcaagctgg cctcggccct gcgattggcc catgcgacag
    13741 cagtcgcgag aaacgccgca atgaaaggct gtgggggaag gggcaaggac cgcggacggg
    13801 accggaagga ctcctggtgc ttctcgaatc aggcggctct tgccgaggct gtcaggacgg
    13861 tctacggttt ggagtatccc gcacatcggc cgaccggtgc tgaaacgccc ggttcgccga
    13921 aaccggagat agccaggcag ccgacgcgac ggtacgatga aaagccgacg gactgaagct
    13981 cttgcaggat aacaaactgt atgtcaaacc tacccgagtt gtccagccgg attccgtttt
    14041 acagcggcca ggtggtttat atctactcat ttgacatcgc ctacgagttc ttgcggcagc
    14101 cggagggcaa cctgctggga caaccgattg tgcaagtctc cgtcggcccg agcaagcgga
    14161 gcccccgaca actggtcttc tatcggccga acatgatcaa cctgccgccg gaggaacggg
    14221 tcgggccgca cgggccggtg accgtcgagc gggcggtcaa gctgctgagc gtcggagcga
    14281 tcagcatcac cgtccgtgta cccttcaagg tgagccgcat cgagcaactg gtggactatc
    14341 acgacctgca attcgccgac ggctcgctga ccgagggggt gcaggaactg gccgagcagg
    14401 tccgaaaaga acttattccc tattgcgatc gaccggtgag tcaggtcggc gaaggcgagg
    14461 cctacacggt tttttgcatt acttcgccgc tggtgaccga ggaaggttcg ctgctcagcg
    14521 ctgaagcgtg gttggaggac cggcgccggc agttggcctc gatcctgaca caggaagagg
    14581 acatgtcgct gttgtcggaa caggaagttg aggagacaac gtcccggtat tgcagctact
    14641 acgggcacga cctcacgatg gtcgactggg atgcggcgat catcatcaat gacccgcagg
    14701 acatggagga aacgctgtac atcatggagc tggccaacgt ccagctcacc gagctggagg
    14761 cctacgaccg gcttctggac gaggccgtga tgcggtcata ccgcgacctc gaatcggccc
    14821 ggcttcgcgg gcgcagcccc gtgatggcgg cgctgcggga gatccgcatc gatctggccc
    14881 ggctgagcga tgagctgtcg aacatcacca agttcttcgg tgactggcac ctggcccgga
    14941 tctaccagat gctttcggag cgatttcacc tcggtgactg gcatcgcgtc gtgggcggca
    15001 agctcaacac gcttgacagc ctttacagcc tgcacaagca ggaccagacc aaccgctgga
    15061 tgctggttct cgaggcgacg atcgtgctgc tcttcgtgct ggacctgatt gtgctggtca
    15121 tggggttggg caagtagacc ccgggaaccc actgcggcga gaatcgggag cttgaacgct
    15181 gccgccggct tcgactcgcc gcgtattcct gccgacgccc gacggaaaac gcctgaaaaa
    15241 acgtttcagt attgtccctg cgtgcgagac ccgatatgct gggaagggcc actgggggat
    15301 agacttgccg ctgtcgggga ggaggctcag gcgtgtcccg aaccgtcgca ttggcccttc
    15361 tactgttcca ttcctgcttg cgcacatcgc cgacagtcga cggccagtgc ctcaactggt
    15421 caccgccgac caacgtcagc cgtgacgtcg ggcattacgc cgtggacggg aatccggcca
    15481 ttgatgcctt cggaaaggtg cacatggtct accaaagctt cctcgacgac tatggagaga
    15541 cgttctacgt caccgacctg agcggtgact ggtcctcgcc tcaatccctt ggttcacagg
    15601 gaggcaaggg atcggccccc aagatcgtca tcacgcccga cgacatgctg cacacgttct
    15661 acggcaaaaa caagctttac tggcgcacca agcccgttag cggcggacag tggagcagtc
    15721 cggcccagat cgacgtcaat cccggcggcg gctcattcat tcagcaggtg acggtcgacg
    15781 gctccggcgg catctacttc atgtacgggc acctctttga cagcagtgcc ccggtgcgaa
    15841 acggcatcta cggtcggtac aagcccctgg gaggcgcctg gaacaccacc gaactcatct
    15901 atggaaacaa cgacgacggc aattggcctc gcggggatga catcgccgcc agaggaaaca
    15961 tgctctggat cgccatcggg gtcgacggag acgcttattt caagaagaaa ccgtcgaccg
    16021 gcgtctggcc ggccggcaag ggtacgaagc tggtggacga tgccggggga atgcgattcg
    16081 ccttcagtcc gatgtccggc gagatcgtcg cgttctacac taagtcgctg ccgtgcaccg
    16141 atccatgtga ggacgatccg tggtttgagg ttttcgccag gttttcccac gacgacggtg
    16201 cgacatggtc cgcgccgcag agcatctccg acaacgttga cgacatcgat cgcacacctt
    16261 cggcggtcta tgactccgcc ggtaatcttc atgtcgtttg ggaaagcttc tgctgcgatc
    16321 acaaactgag aatgcgctat cgcggccgga tgaacggcca atgggacccg acaatcaccc
    16381 gaatcaccag ccacgtgggc gggcacgtcc ccaactcgat caagagcttc ggtagcaact
    16441 tgtttctgac gttctcagac tccggcaccg gcattggaat gtatgacgtg gtcttcagta
    16501 ccgcggccgt cgccgagccc aagcttgctc tcagtccttc gtcactgacg cgcaccatcc
    16561 tcgtcggaag cggtgtcgcc gacgacctgc tggctgtcat gaacgtctgt gtcgggagcc
    16621 tggactacac ggtatccgtg aatgcccctt ggctcgaagt ctcgccgccg gccggctcgg
    16681 tgacgactga aaccgacgcg atcgtcgtga gctacccgca agcgccggcc atgacggccg
    16741 gcatctataa cgccacgatc accgtctccg gaaacgccgt gaacacccct cagaccgtcg
    16801 ctgttcagct caagatccag tcggtcaagc cggattttga cggggacggc gacgtcgacc
    16861 agacggactt cggacacttg caggagtgca tgtccggctt cgccgttccg caaaccgatc
    16921 cggcttgcag caacacattc ctgaacccag gcgatgactt cgtcgatgcg accgagctgg
    16981 gaatctttct cggctgcatc agcggatcgg gcgtccgcgc cgacgtgaca tgcgcggcac
    17041 tccatccgta agtgcgacac cgcaagcttt cgacggccgc agacaggcct ggcgaacgct
    17101 cgccaacggc cacaggggcc ggatcgtcgc tcaagcagag agcttgcggg ccaattcgct
    17161 tctaccgaca atgcactgat gataggctca gagcataggt ctttggcgat caagcacacg
    17221 aggtgaagtc ggcgacgaaa cgatgtcgga ggcagtgaat gcaacaccgc cgcttcggca
    17281 aaacggagat gaacctgtcg gtgctcacgc tggggctcat gcgctacatg agcaaggacc
    17341 tggaccagag tgctcgcgtc gtacggcggg cagtggaact gggcatcaac caccttgaga
    17401 cggcccgcgg ttacgacgac agcgaggaac tgcttggcca cgccctcaag agcatcgatc
    17461 ggggcaaagt ccacatcacg acgaaggtcg gaccgtgcaa gacctatgac gaattcatga
    17521 acaagttcga aaccagcatg agcaagatgg gcatcgacgt gctggacaat ctcgacgtcc
    17581 acggcatcaa caacgaggac aagttccgga tggccatgga cgagaacggc acgcttaaag
    17641 cctgtcgcaa gctgttggat tcgggcgccg tgcggcacat cggcttctcc acccacggca
    17701 agaccgaact tgtgctcaaa acgatcaaca ccggattctt cgaatcgatt aaccttcact
    17761 actattggtt ctatcaggcg catgcgccgg taattgcccg ggcggctgag ttggacatgg
    17821 gggtgctcat catcagtccc aacgaaaagg gtggtatgct gttcaagccg ccggaggtcc
    17881 tgactcggat ctcggccccg ctgcacccga tgaatctgaa ccagcggtgg ctgctgagcg
    17941 acccccgcat ccacacgctg tcgctggggc cggcggttgc cgaggaactc gacgcccaca
    18001 tggccgtggc ggacgacgtc gggccgttga ccgaacaaga gaaggctgcg cttgccaggt
    18061 gggaccggac gatccgtgag aagctgcggg gcgacttctg cacccaatgc ggccggtgcc
    18121 tgccctgccc ggagttcatc gacatcccgg agattctgcg gttgcgaaac ctcgcggtcg
    18181 gactggagat gaacgcctac gggagtttcc gctacaacct gctggacggc agcaacgact
    18241 ggttccacgg ctggccggga agccggtgca cgaagtgcgg ccaatgcctg ccccggtgcc
    18301 ccgagaagct ggacatcccc cgattgttgt tcgatgctca caagcggctc aaaacgggca
    18361 tcggccgccg gttgtggcag aggctcgtcc ggtaggcacg gagtttcgat gttcgtttgg
    18421 gatcccggca acggagaaga ccgttagcca caaaaaacac aaagagtcac aaaaacagtc
    18481 cacggccggt cttggccgtg cgcccattcc gcactgtctg accacggatc acacgcttgc
    18541 ctcgccgcag gcgggggtat cactgataag gactgagcgt catccctcca tccgtgtcat
    18601 cagtgcaatc cgtggttctc ctcccgtctg cagcccggta ggtcgtgctc cgcacgacat
    18661 cctcttgtca ggccaaagga ccaacagccc aaggcggcct tcggccgcaa ccaaagaggg
    18721 cttggtaagc ccctaagggg ctattcttcg tcgctgatgg cctccggcag caatagaccg
    18781 atgacggcgt cggcgataca cacgttggac agaggggctg aacttctcgg ctcgtcaaga
    18841 agggcatcgt gtttgcgggt tcgcgctgac ttccttcttc gcatcgcaag ctttgcccgg
    18901 gatggcgtgt ggccaacgcc ttgggccttg cggaacgacg tcgtatcgcc gagactcggg
    18961 tgtgccgggg ccatgcaatc agaggtgtgt cggtgtcggg agtcatctgt tcgccggtca
    19021 cgatgtcgcc agcccggaat atgataaaga atcctttgag ggaaacgatc atggtcaaat
    19081 cgcagccaac agccgtgtac acattcaagg tcgccctcaa gtactcgcca gacatctggc
    19141 ggctcattga ggttccaggc aatcagacgc ttgaggatct gcacttcgcg atcttcgaag
    19201 ccttcgaacg ttatgacgaa cacctttatt cgttttactt cgagccgccc ggcggatatc
    19261 ggggccggcg ccgcccaagc cgcaacgccg tggaattcac gcatccctac gctgcggagg
    19321 aagccgaccc ttgggatggc cctgcgcccc atgacgcatc ccgaacgacg atcgattcgc
    19381 tcaacctaca tcgggggcgc aagttcgagt accttttcga cttcggagac tcctggtggc
    19441 acgagattac ggtggtgaaa accgacggtc accgccgccg cggccgttac ccgcgaatca
    19501 tcgagaagca cggcaagtcg ccgccgcagt atcccgatta cgacgaaggc atttgctgat
    19561 cggaaaagca tctggagaca acctgtcagg ttctgacaga tgcgctatgg aaaccgagat
    19621 ggggatgtat tcacgtcgcc atgcccttga ggcaccatga gggcattcgc attcaagagc
    19681 gggagagctt cgccggaacg cggaggagca ctttatcctc gagtcgggaa cggatggcac
    19741 cggtaagagg gcctacgggg ctgccctgct gcgacgtcac ggcacgccat tgcgacgcag
    19801 aacgtcgggg agtatcctgc catcggcacg ggcggccggg aagcagagcc agcccttgca
    19861 agcgtatcga atacacagga gcaacggttg tcccatgcgc gatccaagaa tcgacaagct
    19921 cgcacaggtg ttggtgaact actcggtggc cgttaagcgg gatgacgtgg tgcgtatcgt
    19981 cggctcgatc acggccatcc cgctgattcg agcgatctac cgcgaggttg tgatcgccgg
    20041 cggacatccg gttcttcgcg tgactgacga cgagtgcacc gacatcttct tcgagcatgc
    20101 acggcccagg caattggcct atagcaaccc gctgaacatt catgagatca agaacgtgga
    20161 ttgttcgatc agcatctggg caaacgacaa cacacgttcc atgacgcgcg ttcctccgga
    20221 gaaacaagcc gcggtcagca aggcccggcg gcagttcatg gatgtcttca tgaaacgtgc
    20281 cgccatgaaa ggcaagggca agctgcgctg gtcgggtacg cagtttccct gtcactcttc
    20341 ggctcaggat gccgagatgt cgctgtcgca atatgaggat ttcgtttacg gggctggttt
    20401 gctggcctgc aaggacccac ccgccgagtg gcgcaaagtg agcgcccgtc agaggcgggt
    20461 gtgcgacgtg ctcaacaagg cccgcgaggt gcgtttcaag acgctccagg gcacggacat
    20521 ccggtttggc gtgaaaggac ggcgatgggt caactgtgat ggacacgaga acttcccgga
    20581 cggcgaggtc tttaccgggc cgatcgagga ctcggcggag ggggaagtga agttcagttt
    20641 tccggccgtt tatcgcggtc aggaggtgct ggacgtgcgt ttgcggctca gagccggtaa
    20701 ggtggtcgag gcctcagcga gcaagggcga gccgttcctg atccagatgc tcgaccagga
    20761 caagggtgct cgctttgtgg gcgagctggc cctgggcacg aactactcaa tacggcaatt
    20821 cacgcggaac acgctgttcg acgagaaaat cggcgggacc ttccacatgg ccttgggagc
    20881 gggctatcct gaaacgggta gccgcaacaa gagtgcgttg cactgggaca tggtttgcga
    20941 cttgcggcag gggggcgtgg ttgaggccga cggcaaggtc attagcaaga acggccggtt
    21001 cctgagagcc gactggccgg gaccggcagg cagatagcgg gctcggcgac gaggggggcc
    21061 acatgaagcg ggtggtatcc gggattctga tagccgcggt tcatgcggcg atggttttgg
    21121 gcgttgcgcc ggcggcgcgt gcagccgcca catcgcgccc ggccgagacg cattcccaaa
    21181 tggagacccg tcgcgtcagt ttcaccactg acgacggtat cgaaatccac ggcacgtttg
    21241 tgccgtcgcg ggcccaggcg gaagacatgg ctccgatcgc cattctgcta cacatgtaca
    21301 acacggacca taaggacttt ttgccgctgc tgccatttct gcatatggcg ggctttgccg
    21361 cgcttgccat tgacgtgcgt ggtcacggcc agagcgtcgg cccggccgcg atgaacctgc
    21421 cgaagcgggt gggggatcgc gatccaaaac tgtttcggga gatgtatcgt gacgtggagg
    21481 cggcttacct gtggctggct gatcaaccag gagtcgatac ggggcgattt gtgctcgttg
    21541 gtgccagcgt cggcggcagc gtggtgatga actacgccgg ccgtgaccgc tccgtggacg
    21601 ccgttgtgtg gctgacgccc gggacgaagt acctcggcat cgactcgatg cgtgacgccc
    21661 gcaagtacgg ccgtcggccg ctcttgatgc tggctgcaga gcccgaacgg agcgccgccg
    21721 aggcgctggc ggcgattgtg cccggagcca acgtgcaact tttccccgga caggccggcg
    21781 accagatggc cctgcacggt acacgcatgc tgggccaggt ggccgggatc gaaaagacca
    21841 tcgtcgattt tctcacgaag tccgttgggc cgcccgccac gcaacccgtg gtggccagtc
    21901 tcagcagtga tgtctaccac accccggact caccttcggt caagcgaatc aaaaaggcga
    21961 accgccggca gttcagctcg ccgacagagg ccgagactcg aggcctgaag ccctcgaaga
    22021 ccgccgctcg caaaccggct cgatgatcac aggtaaaggg tttccctggg taccccgctt
    22081 ggacggtgcc ggcgggtcac cgcatgacgg acagagtgtg acggcctcgg cggcgttgtc
    22141 gattgatgag tttgcggcct ttggaagtcc gcatacgggc acgaaaacct tgtttacgta
    22201 tcttctttct gcgactgatc ttacgcggat aatgcatggt cctttcgtcc tctgtttctc
    22261 agcttttggt ctctttgatc ccctatgata acggcctgtt gccgtgaacg ccaatctcta
    22321 cgacagcctt ttccgtcggc ggaaaggccg gcgaggctga agtgggcatt gtgagcctcg
    22381 gtatcgggtc ttacgattcg gtgggggcgg tttgtgtgac ggccaggcga ccaagcagct
    22441 cgtccagctt cttagctttg cgatggacga aaagcacggt actcaattcg tgctgggtgt
    22501 tgctgtcgcc gaagtagaag accagcttgc cggttccgag aatcgcgtac tcgacgacgt
    22561 cttcgatgac cagcttgaag cgggcctggg cggtggggtg ccgttcctgt tcgtgcatga
    22621 agccgcggtg gatgatgatc tcgttgtgct cgactttcca gtagttgaag cgggtgatga
    22681 tccaggcgat tccaaggttg aagagcaaca ccatggcgaa cagaaagtag aaccggtcgt
    22741 tggccaccac cctgacgctg agaaagcggc cgatgatcgc ccagatcgat gtttcggcac
    22801 tgcggttgat cagcgccagc acaagtatca ggacggcgat gaggagcgtt accaggataa
    22861 agcctttcag gctgaggtca tacagcaaca tggtcaggtt caccgcgagt accacaagga
    22921 agaagaggcc gatgtagtgt tggacggcac cgaaagtggc gttggcctct gcggtgggca
    22981 tctgatacca gagcatgagg attccgcaga tcagggcggc aaagagcgtt ggcagcatga
    23041 gaatgatctt aggccagggc ctgatgacca gtgcctcgat aggtttaggt ccgacaaagg
    23101 atggctcggc tggggttggc gaatcggcca tgaatcgggt ctcctacctg aaagtcttca
    23161 gcccctcaga cacaacggca atgcagatat gtcggcagat cataaacggg cgccgagcgg
    23221 gaatccagat gggtcaggca ggccggtgaa tagggcgaag ggggagagca agatcgcgcg
    23281 gatggaagct ggtttccggg acaacgcgcc ggtgggcaag ccgctgatgc cactgtgccg
    23341 acgacggacc gatggtcagg agacgttcct gcggattgag atgtcgtgtt ggcaggcgtc
    23401 gtagcactgg ggcgctggga cgatcatgcg agcggcctgg tcgtcgacga ccgtgattcc
    23461 gaagtcctcc agagtctgca aagcgacaat cacttccggc ggccggacgg gccacagcgg
    23521 aacctcaagg aacacgtccc ccgccgctcg tggggcaacg aggagcaagg aggccacagc
    23581 gccgggtgca tcgttgttct gaaccgtcgc ccggccaccg cggaagagtg ccggtccgat
    23641 ctggataccg gacccggacg catcatgatc agggtgaatc tgagctccca gatcggtcag
    23701 cgcgttgatg agcgatgcga gagaggcggt catcgacggc gggttcgtga ggcagtaccg
    23761 gcttcggccc gaaacgcagg ccgccagcag caggtgagcc gttggcgatt gtccccaaca
    23821 gttgagttcg agatcgccgt ggacccagta tccgccgcag ccggtgatct cgatgcagcc
    23881 tgtggcttcg tccgccagga tcatggcgcc aagttgccgc agtccgtcga tcagcgttgg
    23941 tatggccggg tcaaatgccg cgttggtgat tctcgatgag cccctggcca ttgacgcggc
    24001 gaatagcgcc atggcggtag gcgatattgc ggccgccgtc gcgggcatat cgttgccagt
    24061 ccagctcagc ttctcgagtc gctcttccat attcgtcatc ataccctggg tgttcccgca
    24121 agtcttcaat cgaggcatga atcgggtcgg ttcgtcgtat cgacgccggc caggaatccg
    24181 ccggcggcac acacataaga acgtccgtca gatgtgtcga acacttgcga tgggacgtcg
    24241 gattgccgga ggaaaccggg tggctataat ttcgcccgtc gctcaggctg ccgcagggca
    24301 acatggtgcc tgacaggtgc gccgctacgc gaggaccaag agacatgaca cacacggttt
    24361 gtctggtgcc cggagacgga atcggtccgg aagtcacctc cgttgccaag cgggtgatcg
    24421 aagcggcgaa tgtcgatatc gagtgggtca acctgccggc cggcgagacc gggatcgagt
    24481 tcttcggcaa tcccttgcct gagcggacca tcgcggccat catcggccac ggtatcgctc
    24541 tcaagggccc gatcaccaca caggttgcca gcggtcatgg caacgtcagc atcagactgc
    24601 ggcagcggtt ggggctctcg gcagcggtca ggccggtcaa gtccattccg ggcgtgaagg
    24661 ccctccaccc caaggtcgat ctcatcatca ttcgcgagaa tacggagggt ttgtttgccg
    24721 gaatcgagaa cgaggttgct ccaggggtca cccaggcgct gaaggtttgt acggccgatg
    24781 tatcgacgag aatcgccagg ttcgcattcg agtatgccct gtcacacgat cgcagaaaag
    24841 tcaccgtatt tcaccgggcc gatctcctga ggcgcacgga cggccttctc ttgcggtgtg
    24901 cccggaaggc gcaccgctcg ttttccgatc aaatcgtcta tgaagaaatg aacatcgaca
    24961 acggttgcat gcagttggtt cgcaacccct cgcagtttga cgtcatcctg cttgacagcc
    25021 tgaccggggg catggtcagc agcattgcgg ccgggctggt tggtgggccc ggcatggtcc
    25081 cgagtgccag tttcggctcg cagtgcgtgc tcttcgagcc gctgcatggc tcggctccgg
    25141 acatcgcgcg aaaagggatc gccaatccgc tggcttgcat tctcagcgga gcgatgatgc
    25201 tcgaacacct gggagaggac gcggcggcct ctcgtattcg cagtgcctgc gagctggcac
    25261 tcagcacagg tgatgcctca gtgctgacac ccgaccttgg cggttcaggt tcgacagcct
    25321 ctctgactga cgcagtcatt cgagccttga agtgagcaag tcgcgccggc aaaacgagct
    25381 tgtcgtgggt acccgcgggt cgcaactggc ccgcgcccag acgaactggg ttatcgagcg
    25441 gcttcgcgcc gcccacgccg ggctcgaagt ccgcgctgaa atcatcacca caacgggcga
    25501 ccgctttcag gatcggccgt tgccggagat tggcggcaag ggcttgttca ccgaggagct
    25561 tgaacgcgcc ttgctggacg gctcaattga cctggcggtc cactcagcca aggacctgcc
    25621 cacggatctg gccgacgggc tcgatgttct ggcctacccg gctcgcgagg acccgcgaga
    25681 cgcgtgggtt tcggctgatg gagctccctt tgacaagctg cctcctgaga gtgtcgtggg
    25741 gacgaccagc ctgagacgcc aggcgcagtt gctgcatctg cggccggatc ttcggttcgt
    25801 tggtctgcga ggcaacgtgg acacgcgcat ccgaaagatc catcgcggag actgtgcggg
    25861 ggctgtgctg gccatggcgg gcctgaagcg cgcgggtctg gaagagcatg tcacgcatcc
    25921 cttcgacccg caggtgtgtc tgccggcgcc ggggcaaggt gccctggccc tggaaggccg
    25981 caagggcgac gagcgagtgg tatcgctgct ggcggtcgtg catgacgagg cgacggcggc
    26041 cgctgtccgt tccgagcgag cagtcttgtc gatgctggat gccggttgcc gggcgccggt
    26101 ggcgatcctg gcggaagtga tcgccgagcc gatgagtgtt tcagggccga caagtgtatc
    26161 aggcagtcct tccccccttg ccaagggggg accgaggggg attcttcggt gccaggcgct
    26221 ggtccttgat cctaaaggaa cccgccacgt ccgggccaag gccgagcggc cggcaagtca
    26281 gtcggacgat ctggtggccg acatcgtatc ccagctccgc agcggcgggg gagatgaaat
    26341 catcgcgaag tgccgttttg atgaaggaac caggcggtag ggcagtgtcg aggtcgctac
    26401 ggcggccgag acccgtcgtt ttgttgggcg tgggccgctg gaacgagatt cccatctcgt
    26461 ttccccatgt ggtaggattc cagtccgcaa acacgaacac cgctcagcgg atctgaggct
    26521 cgtgctcacg gacggtcacc gctgtttcgg gctgctgacg agctgcaggt cgctgtgggt
    26581 gagatctcgg aacagaaaga tgagcatggg cagcagaggc agcaggcccg cgagcaatgc
    26641 tctgccagca taccaggcca aaaccgccag tggcgacggt gacggccaga gcagctggtt
    26701 caccatctgc tggatcatca atcccgcggt gaagattgcg agattgagaa cagccaacgt
    26761 gcgcacaagc cttggtttgc gccactgcag gatcgcggac agccacagcc agatggcaaa
    26821 cccggcggtg atgaaaatgg caaggacgca gaaagccttg ccgctcgggt aaccctgcag
    26881 ccggaggtag cgaaggatgc cgccgaagcc cgcggcgatc cacgcctcaa gcagccccat
    26941 gttgatgaaa atgacgacga ggagaagcag ccagtccttg cggggattga atcgtgtgcg
    27001 tgacagcagt ggtggtgctt cgtccggtcg tcgagcaagc gaacggcggc ggaggcccgt
    27061 tgtcaggaag atcagcagga gaaacggcgg cagcgcctcc acccattggc tcagccagta
    27121 gagtaacagg tc
//
LOCUS       DAOTPW010000044        27031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1859_length_27031_cov_5.665555, whole genome shotgun sequence.
ACCESSION   DAOTPW010000044 DAOTPW010000000
VERSION     DAOTPW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27031
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1859_length_27031_cov_5.665555"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="P5316_05950"
     CDS             complement(<1..258)
                     /locus_tag="P5316_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HRR84537.1"
                     /translation="MADLEGLAISLIRGKQDEVRAKVQAALDEGVSPVTVLNEGLVAG
                     MSVVGERFRKNLIYVPEVLISARAMRGGMEILRPKLAASGAQ"
     gene            complement(479..1123)
                     /locus_tag="P5316_05955"
     CDS             complement(479..1123)
                     /locus_tag="P5316_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84538.1"
                     /translation="MMSMDLGAYRSLPIPQWLDVNCPKCAYPLRGLPEHRCPECGAEF
                     NIDELVTDTTPLRPPEITARTRPVPQLGLECNGCGYPLRGLPGDQCPECGREFSLTDY
                     IPPEPWGEVAVGASATETMLIFSHLRSLGIPCMITEARGTLGARGTDVFLGGVSKQLR
                     VRRDYYLDALAAIAEATEKTGRPWTCPNCSEEVPGNFELCWKCQHGRGDEPVRN"
     gene            complement(1146..3320)
                     /locus_tag="P5316_05960"
     CDS             complement(1146..3320)
                     /locus_tag="P5316_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRR84539.1"
                     /translation="MTRDEICSKFIESITFDLYSYQEEALMAWFDSPGGVMVCAPTGM
                     GKTLIAEAAVFEALHSRRRLFYTTPLIALTDQKFRELQDLAEKWGFAREEIGLITGNR
                     KVNPDALVRVVVAEILLNHLISPEPGQSDMSEVAAVVMDEFHSFNDPERGIVWELSLA
                     LLPPHVRVLLLSATVGNPYEFCRWLRVEHGREVTLVQSNERRVPLEFIWVGDKLITEL
                     LTEMVSDDDSKCRVPALVFCFNRDECWEVAERLKGLPIISNAQKVEIDAELAAHKDDL
                     ADGVGPKLKQMLIRGVGVHHAGVLPKHKEIVESLFLRKLVPFVVCTETLAAGINLPAR
                     SVVISTLMKGKPGEKKLIFASAAHQMFGRAGRPQFDTQGYVFALAHEDDVRLLKWKKK
                     YDQIPATTKDPGLLKAKKDLERKKPVRSKTEQYWTEGQFKTLIQAGPAKLLSRSMISY
                     NVLIYMLTRTGTLNEVRTFLGKRFESAERVARFREQLDFMLSNLAGLGYITRSDNGEH
                     VTLNDSIRKLQVFRSVDPLYGAFLAEQLNHADGREKILALESVLEIPPTILRRVRIPD
                     APPGPLQTQVIEPQLIQQGLMAAMPQDAPEEDDGDDYWAEEQERPPTFPEMLKLVFES
                     KLAAPEPVYVQPKWVAGGVAEFQGDFYKFVHNRELIKQEGLILRHLLRLTILASEFFA
                     LTEDPEYQSISDAATAACQKVDPRYTSRFLEQAAETRTLTAI"
     gene            complement(3596..4243)
                     /locus_tag="P5316_05965"
     CDS             complement(3596..4243)
                     /locus_tag="P5316_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84540.1"
                     /translation="MYTILTSSLPRILRTSWFRQAVAGYGKLLSQYAATNAATDRDFQ
                     TKYASFWQMRWVRGDTDNSAWLQTYFQILEEAKGKPDVDIEAVARQLYEIPVRRGKKQ
                     LQFSFVTKLRHMRQPTAPIYDANVEAFFMLPRKDQSHPVDERLANLMMNYEFLVKEYD
                     RVISNDLLREAVAACRTELPAMQRITDEKAVDFMIWGFVNVARKGAVRDRTIVYS"
     gene            complement(4313..6367)
                     /locus_tag="P5316_05970"
     CDS             complement(4313..6367)
                     /locus_tag="P5316_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84541.1"
                     /translation="MIILFKCPQCGRAHNVKSELGGRKARCACGQELTIPVVREDDTT
                     ETRRGIALDTTGTASGPVVPAQANSSSQARPDSQGKVRWYDRGLLVLSLLVLFFPVGA
                     YGLWRNSHYSRRSKILLGVTGFALAVAIGQQLTEQVVSAPTDITWKQYMDECGTTAQE
                     VNKTRAIKVFEANYKGKQVRWAGIVDSVSPGLLGGTRIGLKMSPTESFGADVVLKVPD
                     NVDVSALNRGAILECVARIDTQGGPILPHELAWIGPATGATTPLSAQRPTSDTTAVPP
                     HGMVLSQAQIMPATWKDFMSACGTAAQQANEIQAVDLFEKQFKGKVVKWSGVVDRIST
                     SIRGEAQIWMKMSPTESLGPDLVLNAPRNLDLKSIRKGDTVEFLGQIETQGGAFTPHV
                     LYCDQVPVREDGLTGEAGSRLRPEEKTAKDRSSQSMLSSPAQDTSDLARASDLDSHMI
                     RELVRNLNHPDNPVVDAATGELSTYKDKAVPVLKEFILDSTNTTSGRLAAITSLWQIS
                     SQLARSALRDCAMTLAKRTSSLDKALANACNDYARRPSAEHPEDTDNSPDSQDTAGTN
                     TPDSVQKPSEDTPRDQPGSEIDERLNRLVTLKKPYPKVYPNAPTDRITAQYVAILVAE
                     QVGLQYDFKTSFTNTNPKCRQWIYPEFEGIPCNVALKQVLDPLGLTYELKGDRIVLQK
                     KR"
     gene            complement(6935..7465)
                     /locus_tag="P5316_05975"
                     /pseudo
     CDS             complement(6935..7465)
                     /locus_tag="P5316_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(7767..8609)
                     /locus_tag="P5316_05980"
     CDS             complement(7767..8609)
                     /locus_tag="P5316_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84542.1"
                     /translation="MRRLMLFLLIVAFCSGQVCNQQPPDNNDTDNGGGGPATPVDGEA
                     IIADHLAAAAFDDIPAETIQAVKSGYRIWYGHTSHGSQIVTGMQMLFAENSRYAFNAE
                     AGSLVLEEQDDVDLGHEGNLDWTYITRDVLDEPGNNINVVMWSWCGGVSDNTVAGINA
                     YLNAMNELETDYPDVIFIYMTGHLDGTGPSENLYARNNQIRNYCRTHGKILFDFADIE
                     SYDPAGNYYPDGSDWCEWCEDWCSTHTCPTCVECQHSVCFNCYQKGRAFWWLMARIAG
                     WEGT"
     gene            complement(9132..10295)
                     /locus_tag="P5316_05985"
     CDS             complement(9132..10295)
                     /locus_tag="P5316_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84543.1"
                     /translation="MSTATTTRREVLSIFGQTVAGLALIPAPGPALGADAGSGSAIRV
                     GIIGAGNRSGVHIAQLKELPDRCLITAVCDIEQARAEAALVKVGGDAKPFTSHADLLT
                     SRLCDAVVVATPNYTHKQIVVDALKAGCHVLCEKPMATTLDEAREIVAAVRSSKKVFS
                     IGLQFRYASVYRKVRDLVASGTIGDLKYVWAEEFRGDWARLFADPQENARRNWPYYQK
                     LSGGTLLEKNCHDFDILGWVIGSKPVRVTASGGNSVYRERETIDHASVTVDYENGVQL
                     ALGVCMFAKVGRHQTTLVGTDGMIEFPRGGNQLTIRRPKQKDEVLQLVEQTGDSHGQQ
                     GIRELHLDFLDAISTGRPPRTDVNVGYDAMHVPVAAEEAIRDHRVIEFKHVAS"
     gene            10516..12813
                     /locus_tag="P5316_05990"
     CDS             10516..12813
                     /locus_tag="P5316_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84544.1"
                     /translation="MSQRKHTPALLVLVASIVLAASWCVAAGQQGDTASDPVVQSFRS
                     RLEKALDQWCRWLSTYLYRVPGTDLYTLNPTLGTGSNPYRDVAGNTFAAAAAGYWLKR
                     ANPPEDIARPLRGLIKLVLGTHIAANTVDRPDIQKWGATLSMADDWHADLFAVADAML
                     LIDALPPDQREQMLKILVWEADKQVEYGISKKWRTWPGTWPEHSCGESNAWSSTLLQM
                     ARVAMPDSPRQAAWRESAIEYCLNAICMPADVASDKVVGGKPLKERVKAANFEPGGIQ
                     EHHGFYHPGYMGWPLAYLAYAYVMDQQLPESQRNPDVYLHNWKYAYDRLKQATLYTGR
                     FIHCAGDDWITYGYGNTQMMPAYLFAAAHFKDPDATLMADEWIKMIELQQKLGGGSVL
                     AARLSSLLRFRLNDFSWYEGQEGCVLAQALWLLEHMKHEQMVPPASVKEFNERNVGTY
                     YEPNVRLAWHRDSHSFSSVAWRSAFGWWQMLVQPVGLPHLLRYNGNGMGMIQATGVTP
                     QFSLESFKIGTFDEGGFWTTGRIGRETKNVIHGRPNNKVFPMIRQYQAFVALPGGPTL
                     LIDWCMAEDQIWLLREGSLGLRLACDIYNNHQVNLATNGKEHVFKPAECNDTWHDLQA
                     RSITIEKQMAIHALAGEGSFQLMQKRQRYADRSQMVYPEDRFAVDESLLAHELYFGPP
                     AYDRPRIVSPQEWFRKTVLAIYCDPASTPGRPSGTVSGLFPHIVVHLPEAKCAVAVNF
                     TGTTAEIDTPLGRVSVEAESVAVVR"
     gene            12913..13116
                     /locus_tag="P5316_05995"
     CDS             12913..13116
                     /locus_tag="P5316_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRR84545.1"
                     /translation="MKQLQIELPDKIAGEVAALVQAGWFHSQDELVRTALLEFLRHRR
                     LELTEAFQREDIDWALHQRTANP"
     gene            13113..13610
                     /locus_tag="P5316_06000"
     CDS             13113..13610
                     /locus_tag="P5316_06000"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HRR84546.1"
                     /translation="MRRVVSNTGPILRLSEIDALDLLQEVGAVHIPPAVQEELQRALA
                     EWSSRRPEWIQVIDVDSAHMVELQPWMDTEAIHIGEAAALALARQIQADWFLTDDAAA
                     RLVGRTMNLEVHGTIGILLWAAGARRLEAREAEGFLDRLSRSSLWMSPRVLAQARSAL
                     RVICP"
     gene            13669..14172
                     /locus_tag="P5316_06005"
     CDS             13669..14172
                     /locus_tag="P5316_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84547.1"
                     /translation="MGSTVLIIWTAVGMTAGLTVSLLYLGLFQDRSRGRPRCPKCWYN
                     MTGAPSLVCPECGYDARRSKALYCTRRRRWAMVAAVLMAACCYYAWLVRIRVIWLHEP
                     VYLAVRPTSYFLATLPHAQRAQTEYIIKRVQRFGVWPWESVGYHRVRLGTVRADRQSR
                     WHGFSAG"
     gene            complement(14257..15990)
                     /locus_tag="P5316_06010"
     CDS             complement(14257..15990)
                     /locus_tag="P5316_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84548.1"
                     /translation="MLRHSLALLTASVTMTTASAEEFRYKDRLLDDLVRQVPDILKTY
                     DAETGHFGKGIWICTDQNLMFPLAVAYARQGEGNKYYKDQQLLETIMKAGDALIADAD
                     KDGKWVFRKKDGSEWGKIWMPWTYSRWVRAFMLIRDDMPPDRRAAWEKALTLGYDGIA
                     RSQLGHVHNIPAHHAMGLYFAGKVLGKPDWCEKAADFMKKVAGAQFEGGYWSEDVGPV
                     VRYNFVYVDALGTYYSASGDQHVLPAIENALRFHSMFTYPDGSIVETIDERNPYHDGV
                     HPANVGFTLTPHGRALLHVLWAKLGKEPLSADLIASLLMHGEEGPMDPSFLNPGGSLE
                     VLTEKGVDRAAVLRRGPWYVCVSAYTAPIPKSRWHQDRQNFVSVFHEKVGLIVGGGNT
                     KLQPAWSNFTVGDMALLQHKPGDENPDFLPKGPLFHVPSDVRLIREPQLGLDLTYGKQ
                     LCRIRIQPNDDHRLDLVLSAPGVEDGPVAAHLTLIPHIKKPLETAGEQSVTLGDASIA
                     WTPAQVAGAVSHGAARLLVPDTASLFWPALPHNPYRKDGRAEPAEGRIEIRIPFDRQH
                     TEYTVTLEVPE"
     gene            16177..16971
                     /locus_tag="P5316_06015"
     CDS             16177..16971
                     /locus_tag="P5316_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRR84549.1"
                     /translation="MKVVRFEDPSGKVCIGEWIAADQARLISGDLYGSYEITSQTAKI
                     KRLLAPVVPVNVIAIGLNYRRHAAEGGQQVPEFPLVFVKLTTSVIGPGEPIVLPTDAP
                     DEVDYEAELAVVIGRKARKVSEADALKYVLGYTCGNDVSARDCQIRRDKQWARAKSFD
                     TFCPLGPCLLIDPNQDPNAFPIRGRLNGKVMQESNTADMIFSVPKLISYLSHQFTLLP
                     STVIVTGTPEGVGVARKPPVFLREGDTFAVEIDGIGELVNPVVRDE"
     gene            17188..17850
                     /locus_tag="P5316_06020"
     CDS             17188..17850
                     /locus_tag="P5316_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR84550.1"
                     /translation="MPPSAISRLSFVEQAYQAIKQRIVGGDFGPGTQLNIDALARQLG
                     VSNSPVREALRRLENERWVETIPFRGAFVRPLDPVELAGLYELRGMLELAALRKVMPR
                     PPAEGLGQLEKALAELRAAVRRSDPLAYLAADTRFHQAIVDMARNKRLSEMYATLVEQ
                     GKCFMLGRGPESMARYRKGRDQHGAIFDAVRRGDGKRAVRLLEEHLRIGPEDAPVQTR
                     KK"
     gene            18380..20116
                     /locus_tag="P5316_06025"
     CDS             18380..20116
                     /locus_tag="P5316_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84551.1"
                     /translation="MTRDGKLDLKGKEWWPQAAGLEVGEQFIVRAEGEAATRMLVRRE
                     KVTLRDGKEAEALVWIIDDGHDGSAVRGGDRVNDCYVADYGRDGVVDRIVDWIDNDRD
                     GVPDEMDIRYFTNGRLNYCWLGMDFDGDGHMWSLTGYEYGGPSFFEADPYGNNMIYMN
                     KFNPNLGRWSPISECPFAFYDTDGDGQSEEVVRFSAVPLEYDVGKYPDYANTAYALPW
                     KNELERMGVVNIRYSFDIDNLSSAQTPLHYDFGFNLVSKVPYQFPGMYHYNPLRRPPQ
                     ATVVAPYDRVRHISNIYPARETGFSWHENHDDTISIGYGASKDDDFRWEGVFWIWERR
                     FMENTGGPCQKWNVRREWSRKPANRRELYYSGIDRRIHLFGAEEGWIQMGHFAGLGEI
                     GESRMYDTDGNGYFDRWEVWLKGEPGPWRVSTVHDEKARRVPFDIEWLREFYTKKVLP
                     ETMAANEKLMAAMSRVRPFTCSAELQAAMREGPDNYRRYAQDIALELHYRDLRRSLSE
                     AANKVLAEAKMDDLRPLSPEQRAATPNSQTAWELTRLLERLDVAWGEGDIDAVLAALD
                     ELSASPLVRKTD"
     gene            20142..21881
                     /locus_tag="P5316_06030"
     CDS             20142..21881
                     /locus_tag="P5316_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84552.1"
                     /translation="MNILAAGKLYGPDYFIIVAYFVLMLGVGAYFYRYIKAMKDYFAG
                     GNRIPWWLSGVSYYMSSFSAFAFVAYSGLAYKYGIFGVTFYWVTVPAAAFGVVFFATR
                     WRRARISSPVEYLESRYDSAVRQLFAWQGIPQKIIDDALKIVAIGMFLATALNVPLKQ
                     GMFWSSVIMLAYTFMGGLWAVTVTDFLQFVVMMLAVLILFPLSLQAAFDKTGGLSEMI
                     ETAPAGFFKPYTGDFNWVYLATAVVLYCLSYSVNWGLVQRYYCVPTEKDAKKVGWLVV
                     VLNIVTPPLMFMPPALADPNQAYPSLCVTLLPVGLLGLVVAAMFSATMSMLSTDYNVC
                     ASVLTNDVYRRLIRPNASQEQLVLVGRLMTLLVGLISLAVAFYIVSLEGAGGDKLFRW
                     MITLFSVATAPVAVPMLAGLITPKVTTRAALIGFAAGVGVGLFMFFGLEEKFVFLKMT
                     WMRENAIFLVALAVTICLTAAISWLDGQRQGTLPRVEQFLDRLRTPIGELDGDRVEEP
                     ARGDIISPFRIVGTSTAIIGVLLIAITPWTRTRLGLVLDSVLGGFLVVSGVYVAWRSG
                     RGSRTETQLKSQE"
     gene            21887..22912
                     /locus_tag="P5316_06035"
     CDS             21887..22912
                     /locus_tag="P5316_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84553.1"
                     /translation="MTEIGIGVIGSGFMGRTYTETINKYCRRGRVRAVAVGSRAGQLA
                     KDYGIDLEPSVESLLGRKDIQAVFIATPHHVHAEQSIAAAAAGKHILIEKPMACTVAD
                     CDAILDACKKAKVLSSIAFTQRTRICNMRAKQLIEGGRIGTVQQIASTQLLGGGVGTL
                     PGWQSDAANLGTLFGHGIHNFDSIRWLSGQEIKTVYAKCTSFEPDVKVEGTSMVLMTL
                     SGGTFASLWSSFQLPKPSFPRSQFAARIVGEKGLIDLDAYGELRLAVDGEWQVVETQA
                     PIDWQGKGFLDPVRLESYVRQCQDFIDACIENRPPAVTGWDGRQAVAAALAAYESSRT
                     GKEIVLS"
     gene            22909..24207
                     /locus_tag="P5316_06040"
     CDS             22909..24207
                     /locus_tag="P5316_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRR84554.1"
                     /translation="MKFAESLRLYERACKTTPGGIHSNVRANWKPHPMFYERGHGSHV
                     WDADGNEFIDYVLARGPLLLGHSPKPVLDAVKRQLDLGLMYAGQHRLEIEAAERFCKM
                     VPCAEMVRFSSSGSEAVTAAIRLARAVTGRTKILRFEGHYHGWFDNQFWSYAPALDQA
                     GPRENPRPIPATRGQSPADAEHLVIRPWNDLALVEEAFGRHPGQIAAIITEPIMCNMG
                     GIMPRKGYLEGLRELCEKNGALLIFDEVITGFRVSLGGAQEFFGVTPDLATFAKGMSG
                     GFPVSAIAGKHEYMKDFGDLSVSHAGTYNSNAPCMAATVAALDMLAADDGAALKHAHE
                     MGRRLMVGIEDIGRKAGKNVRIRGTPAAFFVSFNDKEEVVDYRTCLSRDREAYTRFWL
                     ALQDRGVRVIPDGLWFVSTAHTQQDVVRTLEAVAEAMKVV"
     gene            24384..>27031
                     /locus_tag="P5316_06045"
     CDS             24384..>27031
                     /locus_tag="P5316_06045"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HRR84555.1"
                     /translation="MSLAAAPAHGQGAKAKLFERVFGDAVRLDPAMVAKVKAAKPGER
                     IFVDRNGDGKNDEAWYIDTAYRHNDRARPLLVRAIDEDGDLDSWMGPDLDSDLYVVDW
                     NADGTVDVVLDYQDNDGDNDVDEMAFYFYMTEHPYFGKDVLRVWWGSDDGDDNLLWYD
                     IDYTYDQHTCQYRCHFSGDESFVAFGLRLEDKEWQSAFENPFLFYDPDGDTCSEVVLR
                     IEGQGNQVRAIRYSFDADDDAYGRRTHDYDFSITARAPEDRPVVLPADATISTKLRGI
                     PTQAWLRRDKAEAFVQNAQWDRVLLTWDEINANTEENVEKDPHERWEGVIAHGNPDFK
                     QVGGPPCSTMNKRYELALKPVSPLKLYYSTADRRLHLKGASKGWLDVDYDFDGKPDAR
                     YVYRDDDHDGIFERRLIDWNGDGTADFDWMVQHGSQHELELAFRAISTFYRGKLNQTL
                     AESQAFVDAARAALGPNDYPEAEAVEDFFLNRLDGWMPRTGLGEYIRRSPAGARFYAD
                     LVRDRLLWSLKQRYGEEEAWKAIESAYGVGNLRTATKYVPREDKEAVTARVNGLSGFA
                     KRISIRMSNSGRTARFDWPVKLGLRRIQTKVPDFNADNCAVVASERWLDWRRIPHQVD
                     EIDPAIGPELSFVADVPASGEATYYLYYSPTGKSETSFARKTSTAEDWVPPNIGWESN
                     RCAYRAYWGQFDFFGKKTDKLIYEDIGKQSYHEETEWGIDALHVGNASGIGGLTLYVD
                     DKPYLVQNPAGKGDVKFSKRQLVKGPVRAAVEITAENIVPEQPDLRVRILCISYAERQ
                     ESEIRATVTGAKGNVLLAPGLVKLPREQAFSDPDKGILGSWGYQQEVIGDIGMAIVVD
                     AANSAQDVVDLPEERRIRCGLTERGEL"
BASE COUNT         5066 a         7843 c         8732 g         5390 t
ORIGIN      
        1 ctgggccccc gaggccgcca gtttgggcct gaggatttcc ataccaccgc gcattgcccg
       61 ggcgctgatc aggacttcgg gaacgtagat caggtttttg cggaaccgtt ctccgaccac
      121 cgacatgccg gcgacgagcc cttcgttcaa cacggtgaca ggcgatactc cttcgtcaag
      181 ggccgcttgg accttggccc tcacctcatc ctgcttgccg cgaatcagcg aaattgccaa
      241 accctcaaga tctgccatga gcgatgctcc aaaccgacct atccgccgat cagcgaatcg
      301 ggcctgaacc tctctgaatg ccggccgagc cactcggccg ggtacgcggg cggcacccgc
      361 ccggccaggg acggcgaaat cagggagata acgtatcgaa ccggccggat ggtgtcaacg
      421 agcccgcgcc ggcaggccag tggccgggga aacacgacgt cgtccgcgat aaggtggctc
      481 agtttcgcac cggctcatct cctcggccat gttggcattt ccagcagagc tcgaagttgc
      541 ctggtacctc ctccgagcag tttggacacg tccaaggccg gccggttttc tcggttgcct
      601 cggcgatggc cgcgagggca tcaagatagt agtcccggcg cacgcggagc tgtttgctca
      661 cccctccgag gaacacgtcg gttccccggg cgccgagagt tcctctggcc tctgtgatca
      721 tgcaggggat gcccaacgag cggaggtgcg agaagatgag catggtctcg gttgctgacg
      781 cgcctacggc cacttcgccc caaggctcgg gtgggatgta atcggtgaga ctgaactccc
      841 ggccgcactc cgggcactgg tcgcccggta gtcctctgag cggatagccg cacccgttgc
      901 attccagacc gagctgaggc acgggccgag tccgggcggt gatctccggg ggccgcaacg
      961 gcgtcgtgtc ggtcacgagt tcgtcgatgt tgaactcagc gccgcattcc gggcaacgat
     1021 gctccggaag gccgcgaagc gggtaggcgc acttcggaca gttcacgtcc agccattgcg
     1081 gaattggcaa agatcggtag gcgccgaggt ccatactcat catcccttgc acccgagatg
     1141 ataccttata tggctgtcag tgtcctcgtt tccgcggctt gttcaaggaa gcggcttgtg
     1201 tagcgcgggt cgaccttctg gcaggcggcg gtggcggcgt cgctgatgga ctggtactcg
     1261 ggatcctccg tgagggcaaa aaactcgctc gccaggatcg tcagacgcag caggtggcgc
     1321 agaatcagtc cttcctgttt gatcagctcg cggttgtgca cgaacttgta gaaatcgccc
     1381 tggaactcgg ccacgccgcc ggccacccac ttgggctgga cgtacactgg ctcgggcgcg
     1441 gccagcttcg actcgaagac cagcttgagc atttcgggga aggtcggcgg acgctcctgt
     1501 tcctcggccc agtagtcgtc gccgtcgtct tcttcggggg catcctgcgg catggccgcc
     1561 atcaggccct gctggatcag ttgcggctcg atgacctgcg tctgaagcgg gccgggcggg
     1621 gcgtccggga tccgcacgcg gcgcagaatg gtcggcggaa tctcaagcac cgactccagt
     1681 gcgagaatct tctcacggcc atcggcgtga ttgagctgct cggccaggaa tgccccgtac
     1741 aacggatcga cgctgcggaa tacctgcagc ttgcggatcg agtcgttgag cgttacgtgc
     1801 tccccgttgt cgctgcgggt gatgtagccc aggccggcga ggttgctgag catgaaatcc
     1861 agttgctcgc ggaagcgggc cacacgctcg gcggactcga accgcttgcc gaggaaggtg
     1921 cgcacctcgt tgagcgtgcc ggtgcgggtc agcatgtaga tgagcacgtt gtatgagatc
     1981 atcgagcggc tgagcagctt ggctgggccg gcctggatga gcgtcttgaa ctgcccctcg
     2041 gtccagtact gctcggtctt gctgcggacg ggctttttgc gctccagatc cttcttggcc
     2101 ttgagcaggc cggggtcctt ggtggtcgcg gggatctggt cgtacttttt cttccacttg
     2161 agcagccgga cgtcgtcctc gtgggccagg gcgaagacat atccctgcgt gtcgaactgc
     2221 ggtcgcccgg cacggccgaa catctgatgg gccgccgacg cgaatatcag cttcttctcg
     2281 cccggcttgc ccttcatcag cgtgctgatc acgacggacc tggccggcag gttgatcccg
     2341 gctgccagtg tttcggtaca aaccacgaac ggcacaagct tgcgcaggaa gagcgattca
     2401 acgatctcct tgtgcttggg cagcacgccc gcgtgatgca cgccgacgcc gcggatgagc
     2461 atctgcttga gcttggggcc gacgccgtcg gccaggtcgt ccttgtgggc cgccagctcc
     2521 gcgtcaatct cgactttctg tgcgttgctg atgatcggca gccccttgag ccgctcggcc
     2581 acttcccagc actcgtcgcg gttgaagcag aagaccaggg cgggcacgcg gcacttgctg
     2641 tcgtcgtcgc tgaccatttc ggtgagcagc tcggtaatca gcttgtcgcc cacccagatg
     2701 aactccagtg gcacacggcg ctcgtttgac tgcaccagag tcacttcgcg cccatgctcg
     2761 accctcagcc agcggcagaa ttcgtacggg ttgccgaccg tggccgacag cagcagcacc
     2821 cgcacgtgcg gcggcagcag ggccagcgac agctcccaga cgatgccccg ctcggggtcg
     2881 ttgaagctgt ggaactcgtc catgaccacg gcggccacct cgctcatatc cgattggccc
     2941 ggctccggcg agatgaggtg attgagcagg atctcggcca ccaccacgcg gaccagagcg
     3001 tccgggttca ccttgcggtt accggtgatc aggccgattt cctcgcgggc gaaaccccat
     3061 ttttccgcca gatcctgcag ctcgcggaac ttctggtcgg tcagggcgat cagcggagtg
     3121 gtatagaaga gccgccgacg gctgtgcagg gcctcgaata cggccgcctc ggcgatgagc
     3181 gtcttgccca tccctgtcgg ggcgcagacc atcacgcccc cgggcgagtc gaaccacgcc
     3241 atcagggcct cttcctgata ggaatagagg tcgaaggtga tggactcgat gaacttggag
     3301 cagatctcgt cacgtgtcat gacaccagca tcgcatctca gcgaagaaat gtcgagccga
     3361 gctcagtgag gccgacccgg cgacgacacg gcttctgcct ggcggagcga tacgattggc
     3421 gttcaccgac tgatctgccg gcggactgcg ggaactggat cgtgtcgccg tggcgcgcgc
     3481 cctcgtcggc cctccctccc cctttggcaa gggggagcgg gcgggggtcg cagcgagggg
     3541 cgagggcgag gggcacaggg cgggaaaggc tttcggggat gtcgcagacc gtggatcaac
     3601 tatacacgat ggtccggtct cgcacggccc cctttcgcgc cacgttcacg aaaccccaga
     3661 tcatgaaatc gaccgctttc tcgtcagtaa tccgctgcat cgctggcagt tcagtacgac
     3721 aggcggcaac cgcttcccgc aaaagatcat tgcttatgac gcggtcgtat tctttgacaa
     3781 gaaactcgta attcatcatc agatttgcca gccgctcatc gaccggatgg ctctggtcct
     3841 tccgcggcaa catgaagaac gcctccacat tcgcgtcgta tatcggggcc gtgggctgtc
     3901 tcatgtgccg aagctttgtc acgaaggaga actgaagctg cttcttgcca cgtcgcacgg
     3961 gaatctcata gagttggcga gcaacggctt caatgtcaac gtcgggtttc ccttttgcct
     4021 cttccagaat ctgaaaatac gtttgcagcc atgccgagtt gtccgtatca cccctgaccc
     4081 atcgcatttg ccagaatgac gcgtatttcg tctgaaaatc ccggtcagtg gccgcattcg
     4141 ttgcggcata ctggctcaag agcttgccgt acccagcaac ggcctgtcga aaccatgagg
     4201 ttctcaggat acgcggcagg ctgcttgtga ggatggtgta catccgatgc tccaattcag
     4261 aatgacggtt tgcctttggg aatcggcata ggactcctct gccgcaacag acctaccgtt
     4321 tcttctgcaa aactattctg tcacccttca actcgtaggt aagaccaaga gggtcaagta
     4381 cttgcttgag agccacgttg catggaatgc cctcgaattc tggatatatc cactgacggc
     4441 atttcgggtt agtgttcgtg aatgaggtct taaagtcgta ttggaggccc acctgctcgg
     4501 ctaccagaat cgcgacatac tgggcagtga ttctgtctgt tggggcgttc ggatacactt
     4561 ttgggtatgg cttcttcaag gtcaccaacc gattgagacg ttcgtcgatt tcagatccgg
     4621 gctggtctct cggtgtatct tcggatggtt tttgaacact gtcaggcgtg tttgtgccgg
     4681 ccgtgtcctg actgtcgggt gagttgtcag tgtcctctgg gtgctccgca ctcggtcgtc
     4741 gtgcgtagtc attgcaggca ttggcgagag ccttgtccag gctgcttgtt ctctttgcta
     4801 gcgtcatagc gcagtctctc agcgcagaac gtgccagttg actagaaatc tgccacagcg
     4861 acgtgattgc tgccagccga ccggaggtcg tgttcgttga gtcaaggatg aactctttta
     4921 ggacgggcac cgccttatcc ttgtaagtac tcaactctcc tgttgcagcg tccacgaccg
     4981 gattgtctgg gtggttcagg ttgcgcacca gctctcgaat catgtgcgag tccaaatccg
     5041 atgcacgagc caaatcggac gtgtcctgcg cagggctgct gagcattgat tgggaggatc
     5101 ggtctttcgc cgtcttctct tcgggcctga gccgcgaacc agcctcgccc gttagcccgt
     5161 cttcccgtac cggcacttga tcgcagtaga gtacatgagg cgtgaatgct ccgccctgtg
     5221 tctctatctg ccccaggaat tcgacggtat cacccttcct aatcgacttg aggtcaagat
     5281 tgcgtggcgc gttaaggacc agatcggggc cgagggattc ggtgggactc atcttcatcc
     5341 aaatctgggc ctcgccccga atactagtgc ttattctgtc gacgaccccg gaccacttga
     5401 ctaccttgcc cttgaactgc ttctcgaaga gatcgacggc ctggatttcg ttggcctgct
     5461 gggccgccgt gccgcaagca ctcatgaagt ctttccaagt ggccggcatg atctgtgctt
     5521 ggctcagaac catgccgtgc ggaggcaccg ctgttgtatc actcgttggg cgctgcgcag
     5581 acaagggcgt tgtcgccccc gtggcaggtc caatccacgc cagttcgtgc gggaggatcg
     5641 gtccgccctg tgtgtcaatc cgggcgacac attccagaat ggcacctcgg ttaagcgcag
     5701 agacatccac gttgtcgggg acctttagga caacgtcggc accgaacgat tccgtagggc
     5761 tcatcttgag cccgatccgc gttcccccaa gtagtccggg cgatacgctg tccacaatgc
     5821 cggcccatct cacctgcttc cccttgtaat tggcctcgaa caccttgatg gcgcgggtct
     5881 tgttgacctc ttgcgcggtc gtgccgcact catccatgta ctgtttccag gtgatatctg
     5941 ttggagcaga caccacttgc tcagtgagct gctggccaat ggctaccgca agtgcaaaac
     6001 cagtgacgcc gagtagaatc ttactccgcc tagagtaatg gctgtttcgc cagaggccgt
     6061 atgcgccaac ggggaaaaag agcacgagca agctgagcac gagcaatcct cgatcatacc
     6121 acctcacttt gccttggctg tcgggacggg cttggcttga agaattggct tgggccggta
     6181 ccacgggacc gctggcggtt ccagtagtgt cgagagcgat accccttctc gtctccgttg
     6241 tgtcgtcttc tctcacgact gggatcgtga gttcttggcc acacgcgcat cttgctttcc
     6301 tcccacccag ttcactcttt acgttgtgag ccctgccaca ttgcggacac ttgaacagga
     6361 taatcatgtc gttcacctcg ccgaagaagg atcggaaaag gcgatccaga tctgtttcgc
     6421 ggatgccttg gcggccttcg tctgctcttt gtcaccttgt ctgatgcggt cattgcgtct
     6481 atccgatcgc cttcacaaga gaagcttcgt cgaagccatg atgggcaaga atagattact
     6541 gctcgagact gttggatgca agggctatgg gctggaggcg gtcggtggtc ggtttccgat
     6601 tcctggtcgc ccccgggaga acggtgctca tcgaggatgc tccaaccgcg cacctcgcat
     6661 ccggcagcgc cttcggaacg gctcccctta gtttccgcca catgactgtg cctgtgggtt
     6721 tcttcctctt ccgtgacgca tcagaaacgg gcgtacgcag ctctttgcag gacccctcgg
     6781 aattcagcat gctaaaccga acagcaacgc ccccaccggc tgcgtgtgca cgccgccgaa
     6841 ttgctgctcc tcccgggcat cgaggctgac ctgaaccccc tttgaacgga agtcgcccgg
     6901 agaaatgcct cagccagcgg caggactcat acgggttgcc gaccgtggcc gacagcagca
     6961 gcacccgcac gtgcggcggc agcagggcca gcgacagctc ccagacgatg ccccgctcgg
     7021 ggtcgttgaa gctgtggaac tcgtccatga ccacggcggc cacctcgctc atatccgatt
     7081 ggcccggctc cggcgagatg aggtgattga gcaggatctc ggccaccacc acgcggacca
     7141 gagcgtccgg gttcaccttg cggttaccgg tgatcaggcc gatttcctcg cgggcgaaac
     7201 cccatttttc cgccagatcc tgcagctcgc ggaacttctg gtcggtcagg gcgatcagcg
     7261 gagtggtata gaagagccgc cgacggctgt gcagggcctc gaatacggcc gcctcggcga
     7321 tgagcgtctt gcccatccct gtcggggcgc agaccatcac gcccccgggc gagtcgaacc
     7381 acgccatcag ggcctcttcc tgataggaat agaggtcgaa ggtgatggac tcgatgaact
     7441 tggaacagat ctcgtcgcgg gtcatgccac cagcatcgcc gcagaatgcg gaaatgtcga
     7501 gcgccgcgtg gcactgggag cttgtctggc agtgtattct gcttctcagc tccgcaatgg
     7561 cgggcccggt gtcgacgagg ctgctcgttt tccgcggcca cgactcccgt tcggccgcat
     7621 cccagaggcg gccaggtttt tataacctta cggccagata tctgtgcggc ttgcgcgcat
     7681 gtttctggat cgcagaacgt ttgggttgcg gttcacaaaa aggcggtccg ccttagtgga
     7741 ggaggcacag cgcctgcttc accttttcac gtcccttccc agccggcgat gcgggccatc
     7801 agccaccaga aggcccggcc tttctggtag cagttgaaac acacggagtg ctggcattcg
     7861 acgcaggttg ggcaagtgtg cgtggaacac cagtcctcgc accattcgca ccaatcggag
     7921 ccgtcagggt agtagttccc ggcggggtcg tagctctcga tgtcggcaaa atcgaacagg
     7981 atcttgccgt gggtccggca gtagttgcgg atctggttgt tgcgtgcgta gagattctcg
     8041 ctggggccgg tgccatccaa gtggccggtc atgtagatga agatgacgtc gggatagtcc
     8101 gtttcgagct cgttcatggc attgagatag gcattgattc ccgccacggt gttgtcgcta
     8161 accccgccgc accatgacca catcaccacg ttgatgttgt tgcccggctc atcaagcacg
     8221 tcacgggtga tatatgtcca gtcaaggttg ccctcatgac cgaggtccac gtcgtcctgc
     8281 tcttccagga ccagggaacc tgcctccgcg ttgaaggcat accggctgtt ctcggcgaag
     8341 agcatttgca tgccggtgac gatttggctg ccgtgcgacg tgtggccgta ccagatgcgg
     8401 tagcctgact taaccgcctg aatcgtctcg gccggaatat catcaaaggc tgcggcggca
     8461 aggtggtcgg cgatgatggc ctcgccgtca acgggcgtcg cggggccgcc gccgccgttg
     8521 tcggtgtcat tattgtccgg aggttgttgg ttgcacacct gacctgaaca gaaagcgaca
     8581 atgagcagaa agagcatcaa acggcgcatg gtatcctcct tttggaaaca ggcgggctga
     8641 cttgcggtcc aggacggatg tgtcaccggc aacgtgcaac ggcggtagtc caggagtccc
     8701 ggatcggttg ccgccgggtc tgctaagaag ctaccccatc tgccgcttgc gggcaagtat
     8761 catcttgaac gcggaaaccc tcacagtggg gccagggttt ttggcgcggt gcagcatccc
     8821 gttgatcggg ccggcagggc aaagggggat caccaggcac agcccagaag aaagaatgtg
     8881 cccgcaagac cacacagatg gacgcaaacg gaaagggaag gtagggcggg ctttgcccga
     8941 cgccgcccgg gccggaaccc cttcgggtcc gtatcttcgg gcggaatcct ctctgccagg
     9001 tgtcgggagc catgcccacg gttttgcgtg ggcatgcggc acggccgcct tacacccgaa
     9061 gtcccgacga gggcgtcggc accgcaagcg gcatctccga aagagaggct catcgatctc
     9121 tggaaggacg attaactcgc gacgtgctta aactcaatga cgcggtggtc gcggatggcc
     9181 tcctcggcgg cgacgggtac gtgcatggcg tcgtagccga cgttgacgtc ggtccggggt
     9241 ggccggccgg tgctgatggc atcgaggaaa tcgaggtgca gctcgcggat tccctgttgg
     9301 ccgtgggagt cgccagtctg ctcgacaagt tgcaagacct catctttctg cttgggcctg
     9361 cggatggtta actggttgcc gccccgaggg aattcaatca tgccgtcggt gccgacaagc
     9421 gttgtctgat ggcggccgac cttggcgaac atgcacacgc cgagggcgag ttggacgccg
     9481 ttctcgtaat cgaccgtcac actggcatga tcgatggtct cgcgttcgcg gtagacgctg
     9541 ttgccgccgg aggccgtaac gcgaaccggt ttgctgccga tgacccagcc caggatgtca
     9601 aaatcatggc agttcttctc cagcagtgtg ccgccggaga gcttctggta gtagggccag
     9661 ttgcggcggg cgttttcctg cgggtcggcg aacagacggg cccagtcgcc gcggaactcc
     9721 tcggcccaaa cgtacttcag atcgccgatc gtccctgatg cgaccagatc gcgcactttg
     9781 cggtagacgc tcgcgtagcg gaactgaaga ccgatggaga aaactttctt gctgctgcgg
     9841 acggcggcaa cgatttccct cgcctcgtcc agagtggtgg ccattggctt ctcgcagagg
     9901 acatggcaac cagccttgag agcgtcgacg acgatctgtt tgtgcgtgta gttgggcgtg
     9961 gcgacgacca cggcgtcgca gagtctggag gtcaacaggt cagcgtggga ggtaaagggt
    10021 ttggcgtcgc cgccgacctt cacgagagcc gcctcggccc tggcttgctc gatatcacag
    10081 acggcggtga tgaggcagcg gtcagggagc tccttaagct gggcgatatg caccccgctg
    10141 cgattgcccg ccccgatgat acccacccgg atcgccgatc ctgaaccggc atccgcgccg
    10201 agggcagggc cgggggccgg gatcaaggcc aagcctgcga cggtctgacc gaagatggag
    10261 agaacctctc ttctggttgt ggttgcagta gacatgcggc atgctcccgg aatgcgcagc
    10321 ggatgcggca aggggccgca gttccgcggc tgagttcctc aacgaattcg cgcgaaccat
    10381 catccaggag ccgtgccggg ttgtccagta gggtcgcagg tattctggaa cgttggtctt
    10441 gctcatgggg cgattctgag gctaccctat gctcttggtc tcaagcgatc gcacgcaata
    10501 cttcaggaga tggtcatgtc gcaacgcaag cacactccgg ccctgttggt gttggtggcg
    10561 agtattgttc tggcggcctc ctggtgcgtg gccgcgggac agcagggaga cacggcatcc
    10621 gatccggttg ttcagagctt ccggtcgcgt ttggagaagg ccctcgacca atggtgtcgc
    10681 tggctctcga cataccttta ccgagtcccg ggaacagacc tgtacacgct gaaccccacg
    10741 ctgggcacgg gcagcaatcc ctaccgtgac gtggccggta acacgtttgc ggccgcggcg
    10801 gcggggtact ggctcaagcg ggccaacccg ccggaggata tcgcccggcc gcttcgcggt
    10861 ttgatcaagc tggtcctggg tacgcacatc gcggccaaca ctgttgatcg accggacatc
    10921 cagaaatggg gggcgacgct gtcgatggcc gatgactggc acgcggatct gttcgcggtg
    10981 gcggatgcca tgctcttgat cgacgccctg ccgccggatc agagagagca aatgctcaag
    11041 atcctggtct gggaggcgga caagcaggtc gagtacggga tcagcaagaa gtggcggacc
    11101 tggccgggaa cctggccgga acactcgtgc ggcgaatcca acgcgtggtc gtcgacgttg
    11161 ctgcaaatgg ctcgcgtcgc gatgccagac tcacctcggc aggccgcctg gcgtgagtcg
    11221 gccatcgaat attgcctgaa cgccatttgc atgccggcgg acgtcgcctc ggacaaggtg
    11281 gtcggcggca agccgctcaa ggagcgagtg aaagcggcca acttcgagcc cggcggcatc
    11341 caggagcacc acggctttta ccatccgggc tacatgggct ggccgttggc gtatctggct
    11401 tacgcgtacg tgatggatca gcaattgcct gagtcgcagc gaaacccgga cgtctacctg
    11461 cacaactgga agtacgcgta cgatcggctc aagcaggcca cgctgtacac agggcgtttc
    11521 atccactgtg ccggcgacga ctggatcacc tacggctacg gcaacacgca aatgatgccg
    11581 gcgtatctgt tcgcggccgc ccatttcaag gaccccgacg cgacgctcat ggccgacgag
    11641 tggatcaaga tgatcgagtt gcagcagaag ctcggcggcg gaagcgtcct ggcagcgagg
    11701 ctttcgtcgt tgttgcgatt ccgtttgaac gacttttcct ggtatgaggg gcaggaaggc
    11761 tgcgtgctgg cccaggcgct gtggctgttg gagcacatga agcacgagca gatggtgcca
    11821 ccggccagcg tgaaagagtt caacgaacgt aatgtcggca cgtattacga gccgaacgtg
    11881 cggctggcct ggcatcgcga ttcccacagc ttttcgagcg tcgcgtggcg gtcggctttc
    11941 gggtggtggc agatgctcgt tcagccggtg ggcctgcctc acttgctgcg gtacaacggt
    12001 aacggcatgg ggatgatcca ggcgacgggc gttacgcctc agttcagttt ggagtctttc
    12061 aagatcggga cgttcgatga gggcgggttc tggacgaccg gacgtatcgg ccgggagacc
    12121 aagaacgtca ttcatggccg gccgaacaac aaggtcttcc cgatgattcg tcagtaccag
    12181 gcgttcgtcg cgctgccggg cggacccacc ttgctgatcg actggtgcat ggccgaggac
    12241 cagatctggc tgctgcgcga ggggagcctg ggcctgcgat tggcatgcga catctacaac
    12301 aaccaccagg tgaacctggc gaccaacggc aaggagcatg tcttcaaacc ggccgagtgc
    12361 aacgacacct ggcatgactt gcaggctcgc tcgatcacta tcgagaagca gatggccatt
    12421 cacgcactgg ccggcgaggg ctcgttccag ttgatgcaga aacggcagcg gtatgccgat
    12481 cgcagtcaaa tggtgtatcc ggaggacagg tttgcggtgg acgagtcgct gctggcccac
    12541 gaactgtact tcggcccgcc ggcttacgac cgcccgcgga ttgtcagccc acaggagtgg
    12601 ttccgcaaga cggtgctggc aatttactgt gatccggcca gcacgcccgg acggccgagc
    12661 ggcacggtca gcggcctgtt ccctcacatc gtcgtgcatc tgccggaggc caagtgcgcg
    12721 gtggcggtca acttcacggg gacgacagcg gaaatcgata cgccgctggg gagagtgagc
    12781 gtcgaggcgg aaagtgtcgc tgtggttcgg tgagggtcgc cattgtctgg cctgcaaccg
    12841 accaacggct aagaacagat tgccctgacc gaggtaaggg tttatcatat gctgttcgga
    12901 aggaatgttg ccatgaaaca actccagatt gaactgcctg acaagatcgc cggtgaggtc
    12961 gcagcccttg tccaagccgg ctggttccat agccaggatg agctcgtacg cactgcgctc
    13021 ctggagttcc tacggcaccg gcggctggaa ctgaccgaag ccttccagcg agaggacatc
    13081 gactgggccc tgcaccagag gacggccaat ccgtgagacg agttgtttca aacaccgggc
    13141 cgattctgcg cctatcagag atcgatgccc tcgatctgtt gcaagaggtg ggtgcggtcc
    13201 atatcccacc ggccgtgcag gaggagttgc agcgggctct tgccgagtgg tcatcacgac
    13261 gccccgaatg gatccaggtg attgacgtcg actctgccca tatggtcgag ctgcagcctt
    13321 ggatggacac ggaagcgatt catataggcg aagcagccgc cttggcgttg gcgagacaga
    13381 ttcaggctga ttggtttctg acagatgacg ccgccgctcg acttgtcggt cgaaccatga
    13441 acttggaggt acatggtacg atcgggatac tgctctgggc tgcgggagct cgccggcttg
    13501 aagcacgtga ggctgagggc ttcctggatc ggctcagccg ctcctcgctt tggatgtcac
    13561 cacgcgttct cgcccaggct cgatcggctc tgcgagtcat ctgcccctga tcatttcaga
    13621 agctccgcgc attgtgaata ccctctgcct ggctgcataa tgctgttcat gggatcgacc
    13681 gtcctgatca tctggacagc cgtcggaatg actgccggcc tcacggtctc gctgctctat
    13741 ctggggctct tccaggaccg cagccgtgga cggccgcgct gcccgaagtg ctggtacaac
    13801 atgaccggcg ctcccagcct ggtttgccca gagtgcgggt atgatgcccg acggtcgaaa
    13861 gccttgtatt gcacccgccg gcggcgatgg gcgatggtgg cagcagtcct catggcggct
    13921 tgctgttact acgcgtggct ggtcagaata cgagtcatct ggttgcatga acctgtctac
    13981 ctcgcggtac gaccgaccag ctatttcctg gcgacactcc ctcatgctca gcgggcacag
    14041 acggagtata tcatcaagag agttcaacga ttcggcgtgt ggccctggga atctgtcgga
    14101 taccatcgtg ttcgcctcgg aaccgttcga gctgatcgtc aatcccgttg gcacggattc
    14161 tcggccggct agcctgccgt gatagctctc gcgggaaaat cgagagctcg gatcaaaaga
    14221 gggccaaggc cactcatcca cctcgcgtcg tcaacgtcat tcaggtactt ccagggtgac
    14281 cgtgtattcg gtatgttggc ggtcgaaggg gattcggatt tcgattcggc cttcggcggg
    14341 ttcggcacgg ccgtctttgc ggtaggggtt gtggggcagg gccggccaga agaggctcgc
    14401 ggtgtcggga acgagcaggc gagcagcgcc gtgtgagact gcgccggcga cctgcgcggg
    14461 tgtccaggcg atggacgcat cgccgagcgt cacgctctgt tcgccggccg tctccagcgg
    14521 cttcttgatg tgcggtatca aggtcaggtg agcggcgacg gggccatcct cgacgccggg
    14581 tgccgacaga accaagtcca gcctgtggtc atcgttcggc tggatgcgga tccggcagag
    14641 ctgtttgccg taggtcagat ccaggcccaa ctgcggctcg cgaatgagtc tgacgtcgct
    14701 gggcacgtgg aacagcggcc ctttgggcag aaagtcgggg ttctcgtcgc cgggtttatg
    14761 ctggagcaga gccatgtcgc cgacggtgaa gttgctccag gccggttgca gcttggtgtt
    14821 gccgccgccg acgatcaggc cgaccttttc gtgaaagacg ctcacgaagt tctggcggtc
    14881 ctggtgccat cgcgacttgg gaatcggcgc ggtgtaggcc gacacgcaga cgtaccatgg
    14941 acctcgccgc aggaccgccg cccggtcaac gcccttctcg gtcaagacct cgagcgagcc
    15001 gccgggattg aggaacgaag gatccatggg gccttcctca ccgtgcatca acagggaggc
    15061 gatcagatca gccgagagcg gctcttttcc cagtttggcc cacagaacat gcagcagagc
    15121 acggccgtgt ggcgttagcg tgaaacccac gttggctgga tggacaccgt cgtgatacgg
    15181 gttgcgctcg tcgatggtct cgacgatcga tccgtcggga taggtgaaca tgctgtggaa
    15241 gcggagggcg ttctcgatag ccggcagcac gtgttgatcg cccgacgcgg agtagtaggt
    15301 gcccagtgca tcgacgtaaa caaagttgta gcggacgacc gggccgacat cctcggacca
    15361 gtagccgccc tcgaactgtg ccccggccac cttcttcatg aaatcggcgg ccttctcgca
    15421 ccaatcgggc ttgccgagca ccttgcccgc gaagtacaag cccattgcgt ggtgcgcggg
    15481 gatgttgtgc acgtggccca actggctgcg ggcgatgccg tcgtagccga gggtcagggc
    15541 cttttcccaa gcggctcggc ggtcgggggg catgtcatcg cggatgagca tgaaggcgcg
    15601 cacccatcgc gagtaagtcc agggcatcca gatcttgccc cactcgctac cgtctttttt
    15661 gcggaagacc cacttgccgt ctttgtcggc gtccgcgatc agggcgtcgc cggccttcat
    15721 gatggtttca agcaactgct ggtctttata gtacttgttc ccctctccct gtcgcgcata
    15781 ggccacggcc agcgggaaca tcaggttctg gtcggtacag atccagatgc ccttgccgaa
    15841 gtgtcccgtt tcggcatcgt aagtcttgag gatatcaggg acttgccgca cgaggtcgtc
    15901 gagaagacgg tccttgtatc tgaactcctc ggccgaggcg gttgtcattg tgaccgaagc
    15961 tgtcagcaag gccaacgaat gtctgagcat ggcatttcct ccaaatgaga gccaaaggtc
    16021 tgaataatag atgatatcag cgtggcagtg cttctgtcac gatgggaggt gtcgagggcg
    16081 gagcagttgc ttttcatggt ggggtttcag tatgttggcc gcacgacgga ttcgcgttcg
    16141 agtctggatt gaccacgcag agaatcggga gtcatcatga aggttgttcg ttttgaagat
    16201 ccgagcggaa aagtgtgcat tggcgaatgg atcgcggcgg accaggccag gctgatcagt
    16261 ggcgatctct atggttccta tgaaatcacg tcgcagaccg ccaagatcaa gcggctgctg
    16321 gctccggtgg tgccggtgaa cgtcattgcc atcggcttga actaccggcg gcatgcggct
    16381 gagggcggcc agcaggtgcc cgaattcccg ctggttttcg tcaaactcac gacaagcgtg
    16441 atcgggccgg gtgagccgat agtgctgccc acggatgccc cggacgaggt ggattacgaa
    16501 gcggagttgg cggtggtgat cgggaggaag gcccgcaagg tgagcgaagc cgatgcgctc
    16561 aagtatgtgc tgggttacac ctgcgggaac gacgtgtcgg cccgcgactg ccagattcga
    16621 cgggacaagc agtgggcaag agccaagagc ttcgatacct tttgcccgct ggggccgtgc
    16681 ctgctgatcg acccgaatca ggatcccaat gcgtttccca tccggggccg gctcaacggc
    16741 aaggtcatgc aggagtcgaa cacggccgac atgatcttct cggtgcccaa gctgatcagc
    16801 tacctctcgc accagttcac tctcctgcca agcacggtga tcgtgaccgg cacgcccgaa
    16861 ggcgtgggcg ttgcccgcaa gcctcccgtt ttccttcgcg aaggtgacac ctttgccgtc
    16921 gagatcgacg ggatcgggga gctggtcaat ccggtggtga gggacgagtg aagtcgtcag
    16981 tggtccgttc tcggttgtca gagtcagcgt gacccatgcg ggtaacagaa cctgatgccg
    17041 agccggggtt caggcttgcc ccggtccctc tccgcgtgca ttcacaggtt tggcggcgct
    17101 gggggttgac aggcagccga tcgttgatta tggatcatgc acgattgggt gcgcttgccc
    17161 attggcttgc tcgggtgatg aacgatcatg cctccttcag ccatttcgcg attgagcttt
    17221 gttgagcagg cgtaccaggc catcaagcaa cgtatcgtcg gcggagattt cgggccgggc
    17281 acacagctca acattgatgc cctggcgcgg cagctcggcg tcagtaactc gcctgtccgc
    17341 gaggctttac gacgactgga gaacgagcgt tgggtggaga caataccgtt tcggggggca
    17401 ttcgtgcggc cgttggaccc ggtcgagctg gccgggctct acgagcttcg ggggatgctg
    17461 gagttggccg cgctgcgaaa ggtgatgcct cgcccgccgg ccgaggggct ggggcaactg
    17521 gagaaggcgc tggccgagct ccgggcggcg gttcgaagaa gcgatcctct ggcctatctt
    17581 gcggcggaca cgcggtttca tcaagccatc gtggatatgg cccggaacaa gagactgagt
    17641 gagatgtacg ccacgctggt cgagcagggc aagtgcttca tgctcgggcg ggggccggag
    17701 agcatggcgc gctatcgcaa gggccgcgat cagcatgggg ccatttttga tgccgttcgc
    17761 cgaggcgacg ggaaaagggc cgtcaggttg cttgaggagc atctgcggat cgggcccgag
    17821 gatgcaccag tgcaaaccag gaagaaatga gagacgtgcc atgcgattgt ggcagacaac
    17881 ggcgaacctc actatgggtc agcacctgcc cgcgaaaggg tggcacggat ccgccgttcg
    17941 cgtatcgtct gcgcaccagc agcctttttg ccccgaaggg gcacagatat gtagcctggg
    18001 gcgcgagccc caagtgcaga tgtcgccagg accgatcagc cccgaagggg cggaagaaga
    18061 gccaggcctt ccacaggcgt ccatcgtccc gctggggcta accggtgaat ggggcatcga
    18121 atccaggggc tcgcgcccct ggctacacac ctccgcccct accggggcga tttgcagccg
    18181 tgcggggtca ctgattgtga agccacaggg ctgctctacg gacccgtccc acccagccgc
    18241 agctcgtagt cggatctctc gccatacgtt gtgtctaaca gccgccgttt tgctgatcgc
    18301 cggatgttcg gccgggcgcg gtcgcggcct ggatgccggg caggaagggc tcggcccctg
    18361 gtggatgcgg gagactgcga tgacccgcga cggcaagctg gaccttaagg gcaaagagtg
    18421 gtggccccag gctgcagggc ttgaggtcgg tgaacagttc attgttcgtg ccgagggaga
    18481 ggcagccact cgaatgcttg tgcggcgaga gaaggtcacg cttcgcgacg gcaaggaggc
    18541 tgaagcactc gtctggatca ttgacgacgg ccatgacggc agtgcggttc gcggcggcga
    18601 tcgggtcaac gactgctacg tggcggacta tggccgcgac ggcgtggtcg accgcatagt
    18661 cgactggatc gacaacgacc gcgacggcgt ccctgacgag atggacatcc gctatttcac
    18721 caacggccgg ctgaactact gttggctggg aatggatttc gacggcgacg ggcacatgtg
    18781 gagcctgacg ggttacgagt acggtggtcc gagcttcttc gaggccgacc cgtacggcaa
    18841 caacatgatc tacatgaaca agttcaaccc caacctcggc cgatggagcc ctatctccga
    18901 gtgcccgttt gctttctacg acaccgacgg cgacggccag agcgaggagg tggttcggtt
    18961 cagcgccgtg ccgcttgaat acgacgtggg gaagtatcct gattacgcaa atacggccta
    19021 cgccttgccg tggaaaaacg agttggagcg catgggcgtg gtgaacatcc gctacagctt
    19081 cgatatcgac aacttgagca gcgcgcagac gccgctccac tatgatttcg gcttcaacct
    19141 ggtcagcaag gtaccctatc agttcccggg gatgtatcac tacaacccgc tgcgacggcc
    19201 gccgcaggca acggtggtgg cgccatacga ccgcgtccgg cacatcagca atatctatcc
    19261 ggcccgcgag acgggttttt cctggcacga gaaccacgat gacacgatct cgatcggcta
    19321 cggggcgagc aaggacgacg atttccgatg ggagggcgtg ttctggatct gggaacggcg
    19381 gttcatggag aacaccggcg ggccgtgcca gaagtggaac gttcgccgag agtggagccg
    19441 caagccggca aaccgccgcg agttgtacta cagcggtatc gacaggcgga ttcatctgtt
    19501 cggagccgaa gaaggctgga ttcagatggg ccacttcgcc ggcctgggcg agatcggcga
    19561 gtcgcgcatg tacgacacgg atggcaacgg gtacttcgac cgctgggagg tctggctcaa
    19621 gggcgagccc ggcccgtgga gggtctcgac ggtgcacgac gagaaggccc gccgcgtgcc
    19681 attcgacatc gaatggctgc gggaattcta cacgaagaaa gtgctgcccg agacgatggc
    19741 ggcgaatgag aagctgatgg ccgcgatgag ccgcgtgcga ccgttcacct gttcggcgga
    19801 acttcaggcg gcgatgcgcg aagggccgga caattaccgc cggtacgcgc aggatatcgc
    19861 cctggagctc cactatcgcg atctgcggcg gagcctgagc gaggcggcta acaaggtcct
    19921 ggctgaggcg aagatggacg acttgcggcc gctgtccccc gagcagcggg cggccacgcc
    19981 caattcgcag accgcctggg aactgacccg cctgctggag cgtctggacg tggcgtgggg
    20041 cgagggagac atcgacgcag tactcgctgc gctggatgag ctttcagcat caccactggt
    20101 gaggaagacg gactgatcgc gaagactggg gaggtgatcg agtgaacatc ctggcagccg
    20161 gcaagctgta cggtcccgac tacttcatca ttgtcgccta ctttgtgctc atgctgggcg
    20221 tgggtgcgta cttctatcgt tacatcaaag cgatgaagga ctactttgcc ggggggaatc
    20281 gtattccctg gtggctgagc ggcgtgtcct actatatgtc gagcttcagc gcctttgctt
    20341 tcgtggccta ctcagggctg gcctataagt acggcatctt cggcgtcact ttttactggg
    20401 tgacagtgcc ggcggcggcg ttcggtgttg tcttcttcgc gacccgctgg cggcgggctc
    20461 gaatcagcag cccggtcgaa tatctcgaat cgcggtacga ctcggctgtc cggcagcttt
    20521 tcgcctggca gggcatcccc cagaaaatca tcgatgatgc tctgaagatc gttgcgatcg
    20581 ggatgttcct ggcgacggcg ctgaacgtcc cgctcaagca agggatgttc tggtcgtcgg
    20641 tgatcatgct tgcctacact ttcatgggcg gtctgtgggc ggtgacggtg accgattttc
    20701 tgcagtttgt ggtcatgatg ctggcggtgc tgatcctgtt tccgctgtca ctgcaagcgg
    20761 cctttgacaa aaccggtggc ctgtctgaga tgatcgagac cgcaccggcc ggtttcttca
    20821 agccgtatac gggggatttc aactgggtct acctggcaac ggccgtggtg ctatactgcc
    20881 tctcttacag tgtgaactgg ggtctcgtgc agcgttatta ctgcgtgccc acggagaagg
    20941 acgccaagaa ggtcggctgg ctggtggtgg ttctgaacat cgtgacccct cctctgatgt
    21001 tcatgccgcc cgccctggcc gatcccaatc aggcctatcc gagtctttgt gtgacgctgt
    21061 tgccggtcgg gctcctgggg ctggtggttg cggcgatgtt ctcggcaacg atgtcgatgc
    21121 tgagcaccga ctacaacgta tgcgccagcg tattgaccaa tgatgtgtat cggcggttga
    21181 tccggcccaa tgcctcacaa gaacaactgg ttctcgtcgg tcggctcatg acgctgctgg
    21241 taggtttgat ctcgctggcg gtggcgttct acattgtcag ccttgagggg gccggcggcg
    21301 acaagctttt tcgttggatg atcactctgt tcagcgtggc gaccgcgccg gtggccgtcc
    21361 cgatgctggc cggactgatc accccaaagg ttacgactcg cgccgctttg atcggtttcg
    21421 cagcgggggt gggcgtcgga ctgttcatgt tctttggact cgaggagaag tttgttttcc
    21481 tcaagatgac atggatgagg gaaaatgcga ttttcctcgt cgccctggcg gtcaccatat
    21541 gcctgaccgc ggccatcagt tggttggacg gacagcggca agggacgctt ccgcgcgtcg
    21601 agcagttcct ggaccgactt cggacgccga tcggcgaact cgatggggac cgcgtcgagg
    21661 aaccggcgcg gggcgatatc atctcaccct tccgcattgt cggcacgagc acggccatta
    21721 tcggcgtgtt gttgattgcg atcacgccct ggacgagaac aagactcggc ctcgtgctgg
    21781 attctgtgct cggcggtttt ctggtcgtca gtggggtata tgtcgcgtgg cgaagcggtc
    21841 ggggctcaag gacggaaacg caactcaagt cgcaggagta ggaatcatga cagagatcgg
    21901 cattggcgtc atcgggtccg gcttcatggg tcggacgtac acggaaacaa tcaacaagta
    21961 ctgcaggcgg ggacgcgttc gggcggtcgc cgtcgggtcg cgggccgggc aactggccaa
    22021 agactacggc atcgacctgg aaccgtcggt cgagtcactg ctgggtcgca aggacattca
    22081 agccgtgttt atcgccacgc cgcaccatgt ccatgccgaa cagtcgatcg ccgccgccgc
    22141 cgccggcaag cacatcctga tcgagaagcc catggcctgt acggtggccg actgcgacgc
    22201 aattctcgat gcgtgcaaga aggccaaggt gctatcctca atcgccttca cgcagcgcac
    22261 gcgaatctgc aacatgcggg ccaagcagct tatcgaaggc ggccgcatcg gcaccgttca
    22321 acagattgcc tcgacgcaac tgctcggcgg cggggtgggt actctgcccg gctggcagag
    22381 cgacgcggcc aacctgggca cgctgttcgg ccacggcatc cacaacttcg actcgatccg
    22441 ctggctcagc gggcaggaga tcaagacggt ttacgcgaag tgcacgagtt tcgaaccgga
    22501 cgtcaaggtt gaaggcactt cgatggtgct gatgacgctg agcggcggga cgttcgccag
    22561 cctgtggagt tcgttccagt tgcccaagcc ttcgttccca agatcgcagt ttgccgcccg
    22621 aatcgtcggc gaaaagggtc tgatcgacct ggacgcatac ggcgagctgc ggctggcggt
    22681 ggatggcgag tggcaggtgg tcgagacgca ggccccgatc gactggcagg gcaagggttt
    22741 tctggaccct gtgcggctgg agagctacgt ccgccagtgc caggacttca tcgacgcgtg
    22801 catcgaaaac cgcccgccgg cggtcaccgg ctgggacggg cgccaggcgg tcgcggccgc
    22861 tttggcggcg tatgagtcgt cgcggacggg caaggagatc gtgctgtcat gaagttcgcc
    22921 gaatcgctcc gtctgtacga aagagcgtgc aagactacgc cgggtggtat tcacagcaac
    22981 gtgcgggcca actggaagcc ccatccgatg ttctacgaac gcgggcatgg gtcacacgtc
    23041 tgggacgccg acggcaacga gttcatcgat tatgtgctgg cacgcgggcc gctgctgctg
    23101 ggtcattcgc ccaagccggt tttggacgca gtgaaacgac agcttgattt gggcctgatg
    23161 tacgccgggc agcacaggct ggagatcgag gcggccgagc gcttctgcaa aatggtaccc
    23221 tgcgccgaga tggtgcgttt ctcgtcgtcg ggcagcgagg cggtaacggc ggccatccgg
    23281 ctggcccggg cggtgaccgg gcggacgaag attctgcgtt tcgaggggca ctaccacggt
    23341 tggttcgaca accagttctg gagctacgct ccggcgctgg atcaggcggg cccgcgagag
    23401 aatccccggc cgatcccggc aacacgcggc cagtcgccgg ccgatgccga gcacctggtc
    23461 atccgtccgt ggaatgacct ggcattggtc gaggaggcat tcgggcgaca tcccggacag
    23521 atcgcggcga tcatcaccga gccgatcatg tgcaacatgg gcgggatcat gccgcgcaaa
    23581 ggttacctcg agggactgcg cgagctgtgc gagaagaacg gggcgctgct cattttcgac
    23641 gaggtgatca cggggtttcg ggtgagtctg ggcggggcac aggagttttt cggtgtgacg
    23701 ccggacctgg ccaccttcgc caaggggatg tcgggcggct ttccggtcag cgcgatcgcc
    23761 ggcaagcatg agtacatgaa ggacttcggc gatctgtcgg tcagccacgc agggacgtac
    23821 aactccaacg ccccgtgcat ggctgcgacc gtggcggcac tcgacatgct ggcggccgac
    23881 gacggggcgg cgttgaaaca tgcccacgag atgggacgac gactgatggt cgggattgag
    23941 gatatcggtc gcaaggcagg caagaacgtc cgaattcgag ggacgccggc ggcgttcttc
    24001 gtgtcgttca acgacaagga agaagttgtg gattaccgca cctgtctgtc gcgcgaccgc
    24061 gaggcgtaca cccgtttctg gctggctctg caggatcgcg gcgttcgtgt gatccctgac
    24121 ggcctgtggt tcgtctcgac ggcacatacg cagcaggatg tggttcggac gctggaagca
    24181 gtggcagagg cgatgaaggt ggtgtgatca tgagagtggt cagttgtcag tggtcagtta
    24241 tcagtcagct attcgcagag ctattggctg ttagctctcg gctattagct ttcaaggcat
    24301 ctcggactgc gttggtcagg cacgcagggc caattcgcaa ttctcaactc ctcattctca
    24361 gttccgccct ggcagtgttg gtcttgtcat tggctgcggc acccgcccac ggacagggcg
    24421 caaaggccaa gctgttcgaa cgggtttttg gcgatgcggt caggctcgac ccggccatgg
    24481 tggccaaggt caaggcggcc aagccgggcg agcggatctt cgtggatcgc aacggcgacg
    24541 gcaagaacga cgaagcgtgg tacatcgaca cggcctaccg gcacaacgac agggctcggc
    24601 cattgctggt acgggcgatt gatgaggacg gcgatctgga ctcgtggatg gggccggatc
    24661 tcgatagcga tctgtacgtc gttgactgga acgcggacgg cacggtcgac gtggtgctcg
    24721 actaccagga caacgacggc gacaacgatg tcgacgagat ggccttctac ttctacatga
    24781 ccgagcaccc ttatttcggc aaagacgtgc tgcgggtctg gtggggcagc gacgacggcg
    24841 acgacaacct gctctggtac gacatcgact acacctacga ccagcacacc tgccagtacc
    24901 gctgccattt cagcggcgat gagtcgttcg tggcctttgg tctgcgtctt gaggacaagg
    24961 agtggcagtc ggccttcgag aacccgttcc tgttctacga tccggacggc gacacgtgca
    25021 gcgaggtggt ccttcggatt gaggggcagg gcaaccaggt gcgggcgatt cgctacagct
    25081 tcgatgccga cgacgatgcc tatggccgtc gaacgcacga ttacgacttt tccatcaccg
    25141 cccgggcccc ggaggatcga ccggtggtgc tgcccgccga tgcgacgatc agcactaagc
    25201 ttcgcggcat ccccacgcag gcctggctga ggcgcgataa ggccgaggcg tttgtccaga
    25261 atgcccagtg ggaccgcgtg ctactgacct gggacgagat caacgcaaac accgaggaaa
    25321 acgtcgagaa agacccgcac gagagatggg aaggcgtgat cgctcatggc aacccggact
    25381 tcaaacaggt cggcggcccg ccatgcagca cgatgaacaa gcgatacgag ttggctctca
    25441 agccggtctc gcccctgaag ctctattact cgacggctga ccggcgcctg cacctcaagg
    25501 gcgcatccaa gggctggctt gacgtcgatt acgacttcga tggcaagccg gatgcgcgat
    25561 acgtgtatcg tgacgatgac catgacggca tcttcgaacg gcggttgatc gattggaacg
    25621 gcgacggaac ggcggacttc gattggatgg tgcagcatgg cagccagcac gagctcgagt
    25681 tggcgttcag ggccatctcg accttttatc ggggcaagct gaatcagaca ctggctgaaa
    25741 gccaggcatt tgtcgacgcc gcaagggccg cgctggggcc gaacgactat cctgaagcgg
    25801 aagctgtgga ggacttcttc ctgaacagac tggatggctg gatgccccgg acgggtctcg
    25861 gcgagtacat ccgccgttcg ccggccggag ccaggttcta cgccgatctc gtccgcgacc
    25921 ggctgctctg gtccctgaag cagcgatacg gcgaagagga ggcgtggaag gccattgagt
    25981 cggcatacgg cgtaggcaat ttacggaccg caacaaagta cgtcccgcgc gaggacaagg
    26041 aagccgtgac ggctcgggtg aacggtctca gcggcttcgc gaaacggatc tcgatcagga
    26101 tgagcaacag cggtagaact gcgcgattcg attggcccgt gaaactgggt ctgaggcgga
    26161 ttcagacgaa agtacccgat ttcaacgccg acaactgcgc ggtggttgca tcggagcgtt
    26221 ggctcgactg gcgtcggatc ccgcaccaag tggatgagat tgacccggca attgggcctg
    26281 agttgagctt cgtggcggac gtgccggcga gcggcgaggc aacctactat ctgtactact
    26341 cgccaaccgg caagagcgag accagcttcg cccgcaagac cagcacggcc gaggactggg
    26401 tgccgcccaa catcggatgg gagtcgaacc gctgcgcgta ccgggcctac tgggggcagt
    26461 ttgatttctt cggtaagaag accgacaagc tcatctacga agacatcggc aagcagagct
    26521 accacgagga gacggagtgg ggcatcgacg ccctgcatgt tggcaacgct tccgggattg
    26581 gcgggttgac gctctatgtc gacgacaagc cttatcttgt ccagaacccg gccggcaaag
    26641 gcgatgtgaa attcagtaaa cgacagcttg tcaaggggcc ggtgcgggcg gcggtcgaga
    26701 tcacggccga gaacatcgtg cccgagcagc cggacctcag ggtgcgaatt ctgtgcatct
    26761 cctacgccga gaggcaggaa tcggagatcc gggcgacagt caccggagcg aagggcaacg
    26821 ttctcctggc tccggggctg gtcaagctgc ctcgcgaaca ggccttttcc gatccggaca
    26881 agggcatctt gggaagttgg ggctatcagc aggaagtcat cggcgatatc ggcatggcga
    26941 tcgtggtcga tgccgccaat tcggcacaag acgtcgtgga cttgcccgag gagcgacgaa
    27001 ttcgctgcgg cctgacggaa aggggtgagc t
//
LOCUS       DAOTPW010000045        26832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1876_length_26832_cov_5.464354, whole genome shotgun sequence.
ACCESSION   DAOTPW010000045 DAOTPW010000000
VERSION     DAOTPW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26832
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1876_length_26832_cov_5.464354"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..4284
                     /locus_tag="P5316_06050"
     CDS             58..4284
                     /locus_tag="P5316_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRR84556.1"
                     /translation="MRDYVSRVSVGEFGIASLKSRIPDLGFRIADGRFACRWAVSLTA
                     LLLLTAATQTLAQQPATARASVAADPAQDAAFVSMLEKANRHHEAKAFHLEAESIEKA
                     IALLPPGHLKRAELANRLAAVYRGSLWRFERALFWVKTIADELGPQPEGINPLFELAD
                     TLFNHGKPDEALAIYRGILKRIMAPDPPWDRNDHRCSRGWRGEELCKFPMGCDPLFPS
                     DLSAAGKLGRLPQLVENRDWRSIRSTLDQNLPLQSLIQQSDGGTTWISLRRWVGESLS
                     SLSAETAQALVAAYEPVLRELADNHDWRALVAFRLSHPIPQLERRVDVMIGDNLLDLG
                     LPALASLYYGRAVRQAGAFDPDLLAREIFCRVQSGESVSPESVPDVVVEAGGAKESLR
                     RHLASWQSSSPGTSVAEPAQLDLAASVVTRLPVLRATLPLRRWQSHWEARAVVESDVP
                     PFAEEFVPLIPLGDSKCLVLNMGDAVQALDLRNNRTLWTFLPPEHNAMTFPSQKYEPQ
                     KTFIECARARRPVVTGNRVYCYLAWGHHDTYRHAGAVFALDRSDGRMLWSSLYLPELA
                     DIMMAGDPAVSRGVVVALAWRPREALPLFFVVGLSAETGELLWLNHLYSGGSLIAYEN
                     QFFIDHPLANAAPVIADGVAYLCTGAGVVAAVDIMDGTTRWAMSYPRLKTMNSAKWAS
                     HMTTSRPAGIVAVFENTVLFAPVDSHVLLAVDRSTGQTRYCREMLDLKAIAAADDERA
                     YLVSGTTISAIRPADGRPVWEQPLPVSGMVGLPTLGPRGLMCPSWEALYVLDAATGAV
                     RAQKNWNREDACGYLCDLGDRLAGGSQTGLNVLMEQKLDEIAPSWLSPETDAKPVEIE
                     VPSRADKWVRWGLPAVDRGDFVLSDDDPDHLLIRSELLQMRNMEPVPTLRWQRQSPLP
                     WGYEATFNRHWVAVWTLDDVFILDAETGRTIWEDHPNKRMNRPIAGVRTVEHQVQVHL
                     GWHGGRALSQTAPTFILFDGQETREVPINDPSMLAATTGQRDGDEQDITYAFEARRIR
                     AFDKDRRQLWQTLTLLDEPRLIKRMGPYVVAVTQDRTFRQNHLRDVSHLRVFDAASGE
                     SLKSIAFPKRWFHLVGQGNGRLLLWDCAFLYCISTPQAVPADRRMITIRQDRPDPDAV
                     AALRMARDLEDPPAFEIPTLTSAPRVDAEFSEWSGVEPHRLSGVMDWTPDFVHRCRTK
                     ARSYAGGEGYAADVRMGLFGEDLYVAVQVSDDIHCTSPVPGLWRSDSVSLIFGEPKGE
                     EIDPLLLTVSLVNGVPRFELGTAVGVFAVSDPTVQDLVSNHGPRRFLLPALSVGVISL
                     PKPTQSLEIAARRDEATRQTWYEFRVPRLLLRYGPDFYWDLLVNENDGAGRAGALQMA
                     SATWGTEETQIGSLRGKGEQPRRRAATPLP"
     gene            complement(4438..6303)
                     /gene="ggt"
                     /locus_tag="P5316_06055"
     CDS             complement(4438..6303)
                     /gene="ggt"
                     /locus_tag="P5316_06055"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRR84557.1"
                     /translation="MRNKPPARLKTCEAAARGGTAETDPPADLMAGRREPRRASWRTC
                     VFGLVSVLALVPIGCGAAPPTYHARPWVKSGRQAMVASDSIHASRAGGEILRSGGNAV
                     DAAVATSLALAVTRPYSTGLGGGGFFMVRLAATGEVFVLDARECAPAAATPDLFTKSR
                     ERWPDAPPLSRFGGLAVGVPGLPAGHRALLERFGTRPLKDLVEPARSLAADGFAIDAD
                     FRGAVRSTLQQIDDHPGLPQRTAALRRALLFDGRVPEIGTILIQPQLARTLQALSAQG
                     LASFYTGDMAAQLVQAVRQDRGVMTAGDLTAYKPVWRKPIRVQYREHFDLFLMPPPSS
                     GGICIAEALSILERWDLRAIHRKDPGLAAHLTVEAMKHAFADRARFLGDADHANVPVA
                     VLTGKPHARSLAARINEQSVADSAAYGQTEPTSALPEDSGTSHFCVVDRWGNIVSATE
                     TINTGFGSLLYVEDLGIVLNNEMDDFTTEPGQVNVYGLRQSDANLVGPHKRPLSCMSP
                     TIVLKDDRPFLAVGGSGGPRIISSVLQVLLNVLEYDLGPGEAVAEPRYHHQWQPDLLY
                     RNDYPADHPVVIGLKRRGHQISDQKRGAVVQAVLVSDDGLIGASDPRKGGRPDGY"
     gene            complement(6534..8768)
                     /locus_tag="P5316_06060"
     CDS             complement(6534..8768)
                     /locus_tag="P5316_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRR84558.1"
                     /translation="MPKLFTLLTAVVSITFATSASADVDAWYPFENNVDDATENGYNG
                     TIIGSPAYAAGVVGQAFDFNGTDNRVEFPGAPGSVGTTVTVACWINSDQQLKRWVVSN
                     MAVGGWGLKLQDNGAFGLVLQTTAGKKTLNTANGVWTAGVWQHWACTFDGAVAKTYKN
                     GVLISTVSSGLAPLSHSSSGVMTVGQEPGDPAGYYDGKLDELAIWNRVLDAGEIAELY
                     ARGVAWPISVTPGLNQNLTALQEGTSPGPIAYTVANGDGLGSHTADIAEVDANGALFD
                     YDWLSLSASQLTVAAHGQEVVTATIDVNPGGTPLSDGLHTAYIKFTDDRSPEPYEIIR
                     QIDLTIIGCRVDATPHAAARAYVLGSNDPVDTVVFTVSNPGKDNVSYDIEKVTPCAWL
                     TIDKSTVGPLNNGDSDTVTATIDPSGLALGTYSCDLCFVTAACSPSDQENARVWLDVV
                     SPSSIISVPGLRAYYRLDDQTVDSSGHEYHGIPVGTIGFADNPLNGTRGLFLPNPLDQ
                     IGDSITVPPEAGAVNTALTLAFFIKPTVVKESWLIQRGFWGAKMTTNSPLTFRFYDTG
                     GARKNVTTTTASQLDTLQHWIVVYTDEGNGTVRWYLNGVLDKEATGLGIMQNPTAVTE
                     MTIGSFTSNAPADEFAGLLDEVMIFDKALNASQALDVYSCGARAVWADADQDKDVDQA
                     DFARFQACMTEVSLPEGREPCRCFDRDRNGTVDMVDFGEFEKCATGPGIAVDPANPPS
                     DCSL"
     gene            complement(9340..9963)
                     /locus_tag="P5316_06065"
     CDS             complement(9340..9963)
                     /locus_tag="P5316_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR84559.1"
                     /translation="MPSDSIVFVVDDDAAVLDSLRFLIESVGLRVRTFSSADEFLAAY
                     TPDQPGCLVLDIRMPGMSGLELQEQLAKRGYTLPVIIITAHGDVPSAVRAMHAGAVDF
                     MSKPFNDQSLLDRVHQALAKDARTRRDEAVRAAIAAKVALLTPREREVMDLVVSGMSN
                     KGIAAQLQLSAKTVETHRARVMEKMEAGSVAELVRMVLTVREPANKG"
     gene            complement(9953..11404)
                     /locus_tag="P5316_06070"
     CDS             complement(9953..11404)
                     /locus_tag="P5316_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR84560.1"
                     /translation="MLVGTVASVATILQLVAVALAVRVWRITGRSPVWLIISIVALLM
                     AARRIMSYSHLLGYHLSHGENADPAELTNEWVGLAISILMVAGIAWAGPLLRSVYESR
                     EALRRQAEDLDHRIRELNCLFGMADVVNRFGASLEDIIRETVELIPKAWRLSDVAYAR
                     VLMDGREYRTSNFKVTSWLMSEPILVFGRSCGEVQVGYLEQRWPENGAPFVPEERRLL
                     TAIAERLGKIAERHQVQEQLNQHREELARVSRLSTMGEMASSLAHELNQPLAAVANYV
                     RGCQRRLGSGTWNESELMQALAEAGNQAERAGQILRSLRDFVAAREPRRVPADINRIA
                     ETAVDLARPEAKVRGIAIRFVPAKDLPPVLADAIQIEQVVLNIVRNALEAMSNTRLNC
                     GEVFVSTSAGGPGEVEVAIQDSGPGLPADAVKKVFDAFYTTKPEGMGIGLSISRSIIE
                     AHRGRLWVTRNEDRGVTFRFALQTCHGENGYAV"
     gene            12259..14247
                     /locus_tag="P5316_06075"
     CDS             12259..14247
                     /locus_tag="P5316_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRR84561.1"
                     /translation="MAHDHLKDSIEAQTGRSTILLIVTLMGGLLVINSFVAEWIFPDK
                     QYSAIFALIGAICLGAPLAFHSLKHIGHGNLPMELLVTLAIGAALSIEKYQEAGVIAF
                     FMVIATLIETRTALGARASIESLIRLTPTKAHRILPDGTEEEVEARLLKAGDLIRVRP
                     GDNIAADGVVVAGESTINQATITGESMPVDKTVNDEVFNGTLNLTGSLDIRVTRAGSE
                     TTLGRVQKLIMNAEQTRIPLMRIIDQYANWYTPTIIMLAFLVWFFIRGPEGMSRAIAM
                     LVVACPCALVLATPTAMVAALSCAARLGILVKDVSTLEGARNLTAIMFDKTGTLTTGE
                     LTVTQMKPAPGVDGATLLKLAASADSLSKHPNAKAVMAVAQKARLSLVRPEQFEEVTG
                     KGVAARINGDEILVGRNTFLADRGVDLGLLNDPNFKEPEGISVLYVSRNGRLIGWIGL
                     EDRTREEAKEAIAELRDLGVRAITMVTGDKWSVARRVAGEMGCTDVYAEVLPSHKLEL
                     VDQMKRKGHRVAVIGDGVNDAPALAAGDLSVAMGAAGSDVAINSASIALMNNDLRRLP
                     FLVRLSRAATRVIWQNMLLGITYIVVLETLGALGKISPMLAAFLHVVASAIVIFNSAR
                     LVRFGEHLQTTEPPSAERPPVKAYGGARSAPSAAPMPA"
     gene            14485..15276
                     /locus_tag="P5316_06080"
     CDS             14485..15276
                     /locus_tag="P5316_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRR84562.1"
                     /translation="MDLATLIGLVAAIGMVVWSLWVGTSGQISVYWDTPSAILVLGGT
                     VFVLLTTQTVERFLRLHKVYMRGFFGKAMTIPETIEKLVTLGEQARREGVLSLENALN
                     EIEDEFLKNGIRLVIDGTAAGEIEQILTSEMEAMDQRHNQGKSIIDMAAKYAPAFGMI
                     GTLMGLVAMLRNMDDPKAIGPGMAVAILTTFYGAIIANVLCLPLSDKLSYRNEEEMLL
                     RTVMMKGILSLQAGDNPRITQAKLAVYLPVDKRALINGVEGKKRQ"
     gene            15306..16130
                     /locus_tag="P5316_06085"
     CDS             15306..16130
                     /locus_tag="P5316_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR84563.1"
                     /translation="MALSKKPAAEGAPAWVLTYGDMMSLLLCFFVLLAAFADYSPGGG
                     TPTPELKKALESIRAAFGAGGTIGPNDEIDYQALIKKLEAISSKETGGYRGDTREEGI
                     YGKHFRLRKIRDGMEITMGGPVVFEPFSDKLTPEGKQALEQIAETVRGHRNKMEIRGH
                     AAEEPRPADWTDDDALQLSFRRAKAVADELVLRGVDRRALVCSAVGANEPLATGVYDV
                     NRRAQARRVEIIVRESLIDDYVKPSSEQDARAKILATPPTSTPSTTPSAPASQDSP"
     gene            complement(16134..16964)
                     /locus_tag="P5316_06090"
     CDS             complement(16134..16964)
                     /locus_tag="P5316_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84564.1"
                     /translation="MGVRFIACFSAVCLICGASGGQASREEGPSASRPATEPASQPAP
                     LDPAVEKILDRLENKDVRDVQADLVYIKKDPLIDYEQKYKGVLRFIEDKPSPRFFIRF
                     DECIVEGVVEKEKEWHVFDGRWYIEVRERSKQVIKREVVGPGEKAEDLFRIGKGPFPM
                     PFGQKKGDIIRHFTIKLVAPGPTDPPNSDHLECTPLPGTELDRRYGKVHFHIDRTLDV
                     PVRVATVAKEDKQEIVASFSNVRLNKGMAASEVNLPSLPGYEVREEPMAEPAGAPTHD
                     "
     gene            17718..18455
                     /locus_tag="P5316_06095"
     CDS             17718..18455
                     /locus_tag="P5316_06095"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRR84565.1"
                     /translation="MRDQADTLGRFLRSAGAEVIEAPTIELGPVEDYRKVDDALRHLD
                     RYQWLVLTSANGVDAMCDRIRAIGLDTRCLARVKIAAIGSATARRLTEQGARPDLVPP
                     EAVGESMAQALVEQGVRGSRILLLVADIARTQLPDALQAAGAQCDSLAIYRTKRPAAL
                     PAVFVERLDRGQIDWITLTSPSSFVNLLWLLGPQHCERLHTVGLASIGPVTTRAIREA
                     GYSVAAEADPHDIPGLVSAIIDASTRD"
     gene            18615..19295
                     /locus_tag="P5316_06100"
     CDS             18615..19295
                     /locus_tag="P5316_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR84566.1"
                     /translation="MSLQYRVISIGTLSRNRFWGETEPKRFPHATTTLIRADNETILV
                     DPSLPAEVLAQRLEERSGLKPEQIQAVFLTTFRPIHRRSLSLFSGATWLMYRPEIEAV
                     QAHLNEMRERAQEGPEDPELCKLLDEEESLVARIEAAPEHLTAQTHLFPAVGATPGSA
                     ALLLALPTQTAVITGDAIVTQEHFAAGQVYEHVAMIEAARKSFEDILEVADEVVPGHD
                     NVFRIGGL"
     gene            19436..19843
                     /locus_tag="P5316_06105"
     CDS             19436..19843
                     /locus_tag="P5316_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRR84567.1"
                     /translation="MEVRMDLARIVISDTSEQQIIVLQEHEGSRQFPIVIGLAEALAI
                     DRRVKNISTPRPLTHDLLANVIRELNGELEKIVIHDLRDHIFFAKLVIRSNGKLIEVD
                     SRPSDAIALGVASDAAIYVTENVLRQVCESPEA"
     gene            19880..21298
                     /locus_tag="P5316_06110"
     CDS             19880..21298
                     /locus_tag="P5316_06110"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84568.1"
                     /translation="MSITGTENGLSTRTDQFLQLCEQRWPAHQPRPAVVTRAPGRLDC
                     MGGMADFSGALALQTTIEQGVYAAAGRRDDQRIRVESVGWNQDGQPSVFEWSLSWFYQ
                     SDGQFVTAETLSSKFDACPWARHVAGVCLALLESGDLPHLAGGVNFLIQSDIPPKAGL
                     ASSAALQVACAKALAALFDLELTAQQLVRACQLANTEVVKNESGLVDHVTCLLGEPDS
                     LLEIRCQPQEVIGSLAMPKCAAFAGVYSGLRLPFYQERYADNRAASLMGRYIIEQILR
                     TSGDPGELNSAYLASIPPNEYVRRFRNYLPPKLRGRDFLDHFGQPPELDIIIRPGQVY
                     KVRSRTEHHIYENDRAHRFMERLARVYRTGDLDTLIEAGELMYASHWSYGQRCGMGSI
                     ETDVLVNCIRERGPACGLYGAKVTGGGCGGTVAVLMADTSAALQALSDACAAYAAKTG
                     RQPMLLSGSSPGAMAFGHRNIG"
     gene            complement(21658..22044)
                     /locus_tag="P5316_06115"
     CDS             complement(21658..22044)
                     /locus_tag="P5316_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84569.1"
                     /translation="MTTRSSIVVIFAFWLLFGALLAGGCGEDSELPEYDQISGKVTRI
                     EKTSGEVSMSYFSEKHRKPVEIHGRLSPEAEIFIDGVTASLDDVRIGDRVKVLGRVEK
                     REGERNLVALKVEIDRREPATSSAPG"
     gene            22275..23117
                     /locus_tag="P5316_06120"
     CDS             22275..23117
                     /locus_tag="P5316_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HRR84570.1"
                     /translation="MADGPSQVEEMFGVGKALVGMVHVGALPGTPLFSRPMPAIIEQA
                     VSEARLLADAGFDGIMIENMHDRPYLRQRVGPEIVAAMTAVLDALRAAVSKPLGLQIL
                     AGANREALAVAAAAGASFIRVENFVFAHVADEGLMSEADAGPLLRYRREIGAEHIRIF
                     ADVKKKHSGHAITADVNIAQAARAAAFFGADAIVVTGPMTGEAATVGDVREVAAAVEI
                     PVAIGSGITPDNLPVFWDLADVFIVGSFIKQDGRWYHPPDPDRVNALIRAVNQLRRVQ
                     RSGL"
     gene            23223..23444
                     /locus_tag="P5316_06125"
     CDS             23223..23444
                     /locus_tag="P5316_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84571.1"
                     /translation="MTRLLRLIMSVGIVVALAGCAATDGGSTQRQASEQPGVAKVSSE
                     TGGEKAGCPKTKCCGEKAAGCCHKPCEKK"
     gene            complement(23609..25384)
                     /locus_tag="P5316_06130"
     CDS             complement(23609..25384)
                     /locus_tag="P5316_06130"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRR84572.1"
                     /translation="MTNHAKLNAWVQEMAAMCRPDKIVWIDGSDAQREQLAAEAVASG
                     ELIKLNQKKYPGCYYHRTAKNDVARTEHLTYICTPRKEEAGPTNNWMPPEEGYRRAAE
                     IFKGSMKGRTMYVIPFSLGPVGSVFSKYGVELTDSIYVVLNMLIMVRCGQAVLDKLGA
                     DGEFTRCLHGKADLDDKRRLILHFPQDNTIWSVGSGYGGNVLLSKKCMALRLASCAAR
                     KEGWLAEHMLILGIEDPSGRIEYVAAAFPSACGKTNLAMLVPPESMTRKGYRIWTVGD
                     DIAWMRIDTDGALWGVNPETGMFGVAPGTNTKTNPNAMATVRRNTIFTNVLLTDNKTV
                     WWEDGDGEPPAKGIDWKGDPWTPDMRDANGKPVLGAHPNARFTAPLSQCPSHSFRTEH
                     HHGVPISAIIFGGRRARLAPLVYQSYSWQHGVFIGSTMASERTAAQYGKQGEVRRDPM
                     AMLPFCGYNMAEYFMHWLQMGQQMTKPPKIFHVNWFRQDVDGQYLWPGYGENLRVLEW
                     VLARCRGEAEATATPIGYVPTPDSLDLGGLNLTQEVLQELLTVDREAWLKETQDIEEF
                     YKLFNDRLPHALRDELASLKRRLMT"
     gene            26408..26542
                     /locus_tag="P5316_06135"
     CDS             26408..26542
                     /locus_tag="P5316_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84573.1"
                     /translation="MERLLSTKQARPAVPAKVDIARKDEPVPDGFLAQLGREQDPKGE
                     "
BASE COUNT         5064 a         8257 c         8303 g         5208 t
ORIGIN      
        1 aatgagaacg tgggggcagg tcggcaagtg tgggcgcggc gaccaccgag gtctctgatg
       61 agggattatg tatctcgagt ctcagttggt gagttcggca ttgcaagtct caaatctcga
      121 atcccggatc tcggattccg gatcgcggac ggccgattcg cgtgccgatg ggcagtatcg
      181 ctgactgccc tcctgctgct gaccgctgcg acgcaaaccc tcgctcaaca accggcaact
      241 gcccgggcat ccgtcgccgc ggatccggca caagacgccg cgttcgtctc gatgcttgag
      301 aaggcaaacc gacaccatga agcgaaggcc ttccatctcg aagccgaatc gatcgagaaa
      361 gccatagctt tgctaccgcc gggtcacctc aagcgagcgg aactggccaa tcggctggcg
      421 gcggtgtatc gaggcagctt gtggcgcttc gagcgagccc tgttctgggt gaaaacaatc
      481 gctgacgaat tgggaccgca gcctgaggga atcaatccgc tgttcgagct tgctgatacg
      541 ctgttcaatc acggcaagcc ggatgaggcc cttgccatct atcgcggcat cctgaagcgg
      601 atcatggccc cggatccgcc gtgggaccga aacgaccacc gctgctccag gggctggcga
      661 ggcgaggaac tctgcaagtt cccgatgggt tgcgatccgc tctttccgtc ggatctgtcc
      721 gcagccggca agctcggcag actgccacaa cttgttgaga accgtgactg gagaagcatc
      781 cgctcgaccc tcgaccagaa cctcccgctg caatcgttga tccagcaatc ggatggggga
      841 actacgtgga tttccctgcg gcgatgggtc ggcgagtcgc tatcaagcct ttctgcggag
      901 acggctcagg ccctggtcgc cgcctatgaa ccggtcttga gggaactggc cgacaatcat
      961 gactggcggg ccctcgtggc gtttcgtctt tcgcatccca ttcctcagct tgagcgtcgt
     1021 gtcgatgtca tgattggcga caaccttttg gatctcggtc ttccggcgtt ggcttcgctt
     1081 tactacgggc gggccgttcg acaggcagga gcctttgatc ccgatctgct ggcccgcgaa
     1141 atcttctgcc gcgttcagtc tggcgagtct gtttcacctg agtctgtccc tgacgtggtt
     1201 gtcgaagcgg gcggcgccaa ggagtcgctg cgccggcatc tcgcctcctg gcagtcgtca
     1261 tcgccgggaa cgtccgtagc ggaaccggca caactggatc tggccgcctc ggtcgttacc
     1321 cgattgcccg tgctgcgcgc gacactgccg ttgcgcagat ggcagtctca ttgggaggcc
     1381 agagctgtgg tcgaaagcga cgttccgccg tttgcggaag aattcgtgcc cctgataccg
     1441 ctcggggatt caaagtgcct ggtgctcaac atgggtgacg ccgtccaggc cttggacctg
     1501 cgcaacaatc ggacgctctg gacttttctg cctcccgaac acaatgccat gaccttcccc
     1561 agtcagaagt acgagcctca gaagacgttc atcgaatgcg cgcgagccag aaggccggtt
     1621 gtgaccggca accgggttta ctgttacctc gcctggggcc accatgacac ctacaggcac
     1681 gccggcgcgg tttttgcgct cgatcgatcc gacggccgaa tgctctggag cagtctgtat
     1741 ctgccggaac tggcggacat catgatggcg ggagatccgg ccgtgagccg aggggtggtc
     1801 gtggcccttg catggcgacc tcgcgaagcc ctgccgctgt tctttgtcgt cggtctgtcg
     1861 gccgagaccg gagaactcct ctggctcaac cacctttaca gcggcggctc gctgattgct
     1921 tatgagaacc agtttttcat cgatcaccct ttggccaacg cggccccggt catcgccgac
     1981 ggagtggctt atctgtgtac gggagcagga gtcgtcgccg ccgtggacat catggatgga
     2041 acaactcgct gggccatgtc gtatccacgc ctcaagacga tgaactcagc caagtgggcc
     2101 tcccacatga ccaccagccg ccccgccggc attgtcgccg tttttgagaa tacggttctc
     2161 ttcgccccgg tcgattctca tgtgcttctg gcagtggatc gttcaacagg tcagacgcgc
     2221 tactgtcgcg agatgctcga tctcaaggcc atcgctgcgg ccgatgacga gcgggcgtat
     2281 ctggttagcg gtaccaccat tagcgcgatc aggcccgcgg atggccggcc cgtatgggag
     2341 caacctctgc ccgtctctgg catggtgggg ctgccgaccc tcggaccgag aggtttgatg
     2401 tgcccgtctt gggaggcgct ctacgtgctc gacgccgcca ccggggcggt ccgcgcgcag
     2461 aagaactgga atcgcgagga tgcatgtggc tacctgtgcg atctgggcga ccgtctcgca
     2521 ggcgggagcc aaaccggcct gaatgtcttg atggagcaaa agctcgatga aatcgcccca
     2581 tcctggttga gccccgagac tgacgccaag ccggtggaaa tcgaggtccc gtctcgagcg
     2641 gacaagtggg ttcggtgggg cctgcccgcc gtggatcgtg gcgatttcgt gctcagtgac
     2701 gacgacccgg accaccttct gatccgctct gaactgctgc aaatgcgcaa catggagccc
     2761 gtgccgactc tccggtggca aaggcaatcg ccactgccgt ggggatatga ggccacgttc
     2821 aatcggcatt gggtggcggt gtggaccctc gacgatgtat tcatactgga tgcggagacg
     2881 ggccgcacca tctgggagga ccacccgaac aagcggatga atcgcccgat cgccggcgta
     2941 cggactgtcg agcatcaggt gcaggtgcat ctcggctggc acggtggcag ggcgttatcc
     3001 cagacagctc cgacgttcat tttgttcgac gggcaggaaa cgagagaggt tccgatcaat
     3061 gacccgtcga tgcttgcggc cacgactggg caacgagatg gagatgaaca ggatattacc
     3121 tatgctttcg aggcacgacg catccgggcc ttcgacaagg atcgccgcca gttatggcaa
     3181 accctgaccc tgcttgatga gcctcgcctg atcaagcgga tgggcccgta tgtcgtcgcc
     3241 gtcactcaag atcgaacgtt ccgtcagaat caccttcggg acgtaagcca tcttcgcgtt
     3301 ttcgacgccg cgagcggcga gtccctcaag agcatcgcgt tccccaagcg atggttccat
     3361 ctcgtgggac agggcaacgg gcggctactg ctctgggatt gtgccttctt gtattgcatt
     3421 tcgacgccac aagccgttcc tgctgaccgg cggatgatca ccatccgcca ggaccggccc
     3481 gatcccgatg cggtcgccgc attgcgtatg gcacgtgatc tggaggatcc tccggctttc
     3541 gagattccaa ccttgacgtc ggccccgcgt gtcgatgccg agttttccga atggtccggc
     3601 gttgagcccc accgattgag cggtgtgatg gattggacgc ctgattttgt ccatcgttgc
     3661 aggacgaagg cacgctcgta tgccggcggc gaaggttacg cggccgatgt tcgcatggga
     3721 ctcttcggcg aagatctcta tgtggctgtg caagtcagtg atgatattca ctgcacctca
     3781 ccggttccgg gactctggcg gtccgacagt gtaagcttga tcttcggcga gccgaaaggc
     3841 gaggaaatcg atccgttatt gcttactgtc tcactggtca acggtgtgcc gaggtttgag
     3901 ttgggcacgg cggtcggcgt ctttgccgta tctgatccga cggtccagga tctcgtgtcg
     3961 aatcatgggc ctcggcgctt cctgctgccg gccctgagcg ttggggtgat ctcgctgcca
     4021 aagccgaccc agtcgttgga gattgcggcc cgccgcgacg aggcaacgcg acaaacctgg
     4081 tacgaattcc gtgttcccag gctcctgctg cgatacggcc ctgatttcta ctgggacctg
     4141 cttgtcaacg agaacgacgg tgccggacgt gctggtgcct tgcagatggc ctcggcaacc
     4201 tggggcaccg aggaaaccca gatcggcagc ctgcggggca agggtgaaca accccgccgc
     4261 cgagccgcca cacctttgcc ataatccgct tctgccggcg aggagtgagc ggcgggatac
     4321 cgatcgagcg gaacccaccg gcgggagcgc tggcaaatgt gatagaagac ccaatcgaga
     4381 tggaaatcct cgccatcgac cttgaaatgc acctggcggc tcacgaagac aaagccgtca
     4441 gtatccgtcg ggccggcctc ctttgcgtgg gtcgctggcc ccgatgaggc cgtcatcgct
     4501 gacgaggaca gcctgaacaa cggcaccgcg tttttgatcg gaaatctggt ggcctcgccg
     4561 cttgagaccg atcacgaccg ggtgatcggc ggggtaatcg tttcggtaga gcaggtcggg
     4621 ctgccattga tggtgatacc ttggctcggc aaccgcttcg ccgggaccga gatcatactc
     4681 aagaacgttg agcaagacct ggaggacgga tgaaatgatc ctcgggccgc cggaaccgcc
     4741 gacggcaaga aaaggtcgat cgtccttcag gacgatggtc ggcgacatgc atgaaagcgg
     4801 ccgcttgtgc gggccgacaa ggttcgcatc tgactgtcgc aggccgtaga cgttgacctg
     4861 gccgggctca gtcgtgaaat catccatttc gttgttcaag acaatcccga ggtcctcgac
     4921 gtagagcaac gaaccaaagc cggtgttgat cgtctccgtg gccgacacga tgttgcccca
     4981 gcgatccacc acgcagaaat gcgaagtccc gctgtcctca ggcaaagcgg atgtcggttc
     5041 cgtctggccg taggcagcgg agtcggcgac cgactgctca ttgatgcgag cggcaaggct
     5101 ccgggcgtgg ggtttgccgg tcaacacggc gacagggaca ttcgcatggt ccgcgtcgcc
     5161 cagaaaccgg gcgcggtcgg caaacgcatg tttcattgcc tccaccgtca gatgtgccgc
     5221 caagccggga tccttgcggt gaattgctct caagtcccag cgttcgagaa tactgagagc
     5281 ctcagcgatg cagatgccgc cggacgaggg cggcggcatg aggaagagat caaaatgttc
     5341 gcggtattgc acacggatcg gtttccgcca gaccggtttg taggcggtca gatcgccggc
     5401 ggtcatgaca ccgcgatctt gccggacggc ttgaaccaat tgggcggcca tgtcgccggt
     5461 gtaaaaagac gcgaggcctt gagccgagag cgcctggagc gtgcgtgcca gttgcggttg
     5521 tataaggatg gttccgattt caggcacgcg gccatcaaac aacagagctc gacgcagtgc
     5581 cgccgttcgc tgcggcaggc ctgggtggtc gtcgatctgt tgcagggtac tcctgaccgc
     5641 gccgcgaaaa tcggcgtcga tcgcgaagcc gtccgccgca agtgaccggg ccggctcgac
     5701 aaggtccttc aatgggcggg taccgaaacg ttccagcagt gcgcgatggc cggccggcag
     5761 gcccggtacg ccgaccgcga gacctccaaa tcggctcaga ggaggggcgt cgggccatcg
     5821 ctcgcggctc ttggtgaaaa ggtcgggcgt cgcggcggcc ggggcacact cgcgagcatc
     5881 gagaacaaac acttcaccgg tcgcggcgag acgcaccatg aagaagccgc ctccgccgag
     5941 gcccgtgctg taaggccgcg tgaccgcaag ggccagcgag gtggcaaccg ctgcgtcgac
     6001 cgcgttgccg ccggaacgca ggatctcgcc cccggcacgg gaggcgtgga tgctgtcgct
     6061 ggcgaccatg gcctgacggc cggattttac ccagggccgg gcgtgatacg tcggcggcgc
     6121 cgccccacag ccgatcggga cgagagcaag gacgctgacc agtccaaaga cgcaggtcct
     6181 ccacgaggcg cgtcttggtt cgcgccggcc ggccatcaga tcggccggcg ggtcggtctc
     6241 agccgtcccg cctcgggcgg ctgcctcaca ggttttgaga cgcgcagggg gtttgtttct
     6301 catactcagc gactcggccg ggcgcacctg cagggcatca acccacagcc ttctccttgg
     6361 cagcctcgtg cgccctgaag cccaccagac ttcagggtcg aaggccgcag cacaccgggt
     6421 cggcgagact atggtaacag aatccgaacg aagaaacccg caacccggtt ctatccttca
     6481 ttctcacaac cgccctctgg cgagcttgcc ccgggccgac ctcaaatgcg atcctacagg
     6541 ctgcagtcgc tcggcgggtt tgccgggtca actgcgatac cgggccccgt cgcgcacttc
     6601 tcgaattcgc cgaagtccac catgtccacg gtgccattgc gatcgcgatc gaaacaccgg
     6661 cacggctcgc gtccctcagg aagggagact tcggtcatgc acgcctgaaa ccgggcgaag
     6721 tccgcttggt ccacgtcctt gtcctggtcc gcgtcagccc agacagcgcg ggccccgcac
     6781 gaatacacgt ccagcgcttg agaggcgttc aaggccttgt cgaaaatcat cacctcatcg
     6841 agcagcccgg cgaattcgtc cgccggcgcg ttcgaggtga aggaaccgat ggtcatttcg
     6901 gtgaccgccg tggggttctg catgatgccc agaccggtcg cttccttatc caggactccg
     6961 ttgaggtacc atctgacggt gccattgccc tcgtccgtat agaccacgat ccagtgctgg
     7021 agggtgtcca actgcgaagc tgtagtagtc gtgacgttct tccttgctcc tccggtgtcg
     7081 tagaacctga acgtcagggg cgagttggtg gtcattttcg ccccccagaa tcctcgctga
     7141 ataagccagg attccttgac cacagtgggc ttaatgaaaa acgccagggt cagtgccgta
     7201 ttgaccgcac ctgcctcggg cgggacggtg atcgaatccc cgatctggtc caggggatta
     7261 ggcaggaaca accctcgagt cccgttcagc ggattgtcgg cgaagccgat cgtgcccact
     7321 ggaatgccgt ggtactcgtg accagaggag tcaaccgtct ggtcgtcgag ccggtagtag
     7381 gcccgaagcc ccggcacaga gatgattgat gacgggctga cgacgtcgag ccagactctg
     7441 gcgttttcct gatccgaagg actgcatgcg gcggtgacaa aacagaggtc gcaagaatag
     7501 gttcccagag caaggcccga cgggtcgatc gtcgcggtca ccgtgtcgga atcaccgttg
     7561 ttgagcgggc cgaccgtgct cttatcgatc gtcagccacg cgcacggggt cactttctcg
     7621 atgtcgtacg agacgttgtc cttcccgggg tttgagacgg tgaagaccac ggtgtccacc
     7681 ggatcatttg aacccaggac gtaggcgcgc gccgctgcgt gcggagtcgc gtcgaccctg
     7741 caaccgatga tcgtcaggtc gatctgccgg attatctcgt agggttcggg actgcggtcg
     7801 tcggtaaact tgatataggc cgtgtggaga ccgtccgaca gcggcgtccc gcccgggttg
     7861 acgtcgatcg tcgcggtgac cacctcctgg ccgtgggccg cgacagtcaa ttgcgatgcg
     7921 ctcaacgaca gccagtcgta gtcgaataga gccccgttcg catcgacctc ggcgatatcg
     7981 gccgtatgtg aaccaagccc gtcgccattg gccaccgtat aggcgatagg tcccggggac
     8041 gtgccttctt gcagggcggt gaggttctga ttgagacccg gcgtgactga gatcggccaa
     8101 gcaacaccac gggcgtaaag ctcggcgatt tcacccgcgt cgagcacgcg gttccatatg
     8161 gccagctcat ccagcttgcc gtcatagtag cccgcggggt ctcctggctc ctgcccgacc
     8221 gtcatcacac cgctcgagct gtggctcagc ggagccaggc cgctggacac agtgctgatc
     8281 agtaccccgt tcttgtaagt cttggctacc gctccgtcaa aggtgcaggc ccagtgttgc
     8341 cagacgccgg cggtccagac gccgttggct gtgttcaggg ttttcttgcc ggcggttgtc
     8401 tgcagaacca gcccgaacgc cccgttgtcc tggagcttga ggccccaccc gccgaccgcc
     8461 atgttgctca ccacccacct cttgagctgc tggtcactgt tgatccagca ggccaccgtg
     8521 acggttgtgc ccacagagcc gggggccccg gggaactcca cacggttgtc cgtgccattg
     8581 aagtcgaatg cctggcccac cacccccgcc gcataggccg gcgaaccaat aatcgtcccg
     8641 ttgtagccgt tctcggtagc atcatcgacg ttgttctcga acggatacca cgcatcgacg
     8701 tccgctgatg cacttgtggc aaaggtgatg ctcaccacgg ccgttagtaa tgtgaaaagc
     8761 ttaggcatga caacctcctt cctgaaccag atgctttgga cagacgagca gtaattccgc
     8821 ctcgataaat cacaccccgc cgatgaatgc cgccgctggg ccatgcggcc tgcaatcaaa
     8881 gcggctttcg gcccgtgtgg gtttcaggct tgtcttatcc ggctacccgt tttccctgat
     8941 ctccgattcg gacgggtcct gggtcatccg gcgaaccgcg agaccctttg tgcgcacgca
     9001 cggtttcgcc tcggccccgg caactctgtg tgctccggta ctcgtcgctc cccaaaccgg
     9061 gggtcgatct cgaacgatca ataagaattg actgtcgccg ccgacagcaa aaacgattct
     9121 acgactccga ttcccgaagc gtcaagacaa ttcctgtcga cttcggtgtt cctgagggtt
     9181 catccgtcgc cccgcaacgg gcggtgccgg cgttgtgctg aggtcaagcc gtcggcggga
     9241 cacggacgtc ctggagtaca tcagcaaaag atccattcaa actgatatgc gggtccggtc
     9301 gaggagcccg gttggggagg gacgccggct gaacggtgct catcccttgt tggcgggttc
     9361 tctcaccgtc aagaccatgc gtacgagttc ggccacgctg ccggcttcca tcttctccat
     9421 gacgcgagcg cggtgggtct caacggtctt agcgctgagc tgcagttggg cggcgattcc
     9481 cttgttggac atcccggata cgacaaggtc cattacctcg cgctcgcgcg gggtgagcaa
     9541 agcaaccttc gcagcgatgg cggcgcgaac ggcctcgtcc cgacgagtcc gggcatcctt
     9601 ggccagcgcc tggtggacgc ggtccagcag gctctgatcg ttgaacggct tgctcataaa
     9661 atcgacggcc ccggcgtgca tggcccgtac ggcagagggt acatcgccgt gggcggtgat
     9721 gatgatcacc ggaagtgtgt agccgcgctt cgccagttgc tcctggagtt ccagaccgct
     9781 cattcccggc attcgaatat ccaacaccag gcagccgggc tgatcgggcg tgtaggcagc
     9841 gaggaattcg tccgcagagg agaacgtccg gacccgcagg cccaccgact cgatcagaaa
     9901 acgaagcgaa tcaaggacgg cggcgtcatc gtccaccaca aagacaatgg aatcagacgg
     9961 cataaccgtt ctctccatga caggtctgga gcgcgaagcg gaaggtcacg ccccggtcct
    10021 cattccgcgt cacccacagc cggccccgat gagcttcgat gatcgagcgg ctgatcgaca
    10081 gaccgatgcc catgccctcg ggctttgtcg tatagaaggc gtcaaacacc ttctttacgg
    10141 cgtcggccgg caagccgggc ccggaatcct gaatggcaac ctccacctcg ccgggcccgc
    10201 cggcactggt gctgacaaac acttccccgc aattcaggcg ggtgttgctc atcgcctcca
    10261 gcgcgttgcg aacgatattg agcacaacct gctcgatctg aatcgcatcc gccaacaccg
    10321 gcggcagatc cttggccggc acgaatcgga tggcgatgcc tcgcacttta gcctccggcc
    10381 tcgccaggtc aaccgccgtc tcggcgattc tgttgatgtc ggcaggaacc cgtcgaggct
    10441 cgcgtgcggc cacaaagtcc ctcaggctgc ggaggatctg cccggcccgc tccgcctggt
    10501 tcccagcttc ggccagggcc tgcatcagct cgctctcatt ccaggttccc gagcccagtc
    10561 gtcgttggca tccgcggacg taattggcga ccgcagccaa gggctggttg agctcatggg
    10621 ccagactgga ggccatctct cccatggtgc tgagacgcga gacgcgagcc agttcctccc
    10681 ggtgctggtt cagttgttcc tgaacctgat gccgctccgc gatcttgcca agtcgctccg
    10741 caatggcggt cagaagtcga cgctcttctg gaacaaaagg agcaccattc tcaggccacc
    10801 gctgctccaa atagcctact tgaacctcgc cgcaggaacg cccgaaaacc agaatgggtt
    10861 cgctcatcaa ccatgaggtg accttaaaat tgctcgtccg atactcgcga ccatccatga
    10921 gcacgcgggc ataggcaaca tccgacaatc gccatgcttt cgggatcaat tccaccgtct
    10981 cgcggatgat gtcctcgagt gaggcgccaa accggttaac cacgtcggcc ataccaaaca
    11041 ggcagttcag ctcgcggatg cggtgatcca gatcctcggc ctggcggcgg agagcttcgc
    11101 gtgattcgta aaccgacctg agcaatggtc cggcccacgc gatgcctgcc accatcagaa
    11161 tggatatagc cagtccgacc cactcgttgg ttagctcggc aggatcagca ttctcgccgt
    11221 gcgacaggtg gtagccaagc aggtgcgagt aactcatgat gcgtcgggcg gccatcagca
    11281 gggcgacgat ggagatgatc agccaaacgg gagatctccc ggtaatgcgc caaacgcgaa
    11341 ccgccaatgc gacggcaact agctgcagga ttgttgccac tgaggcgacg gtcccgacaa
    11401 gcatacgcga ggctccacca gtctcagagg atgccgaaga gacccgcaag atcggcacga
    11461 atgccccgga ctgggacagt cctgtggctg cggctcccga aagggcaaac ccatgccggt
    11521 ttctcacctc gggtatggta accggttgcc aagcgagccg cgagttcaat gagactcaat
    11581 gggcaggccc atgccccggg tttcggatca cccctgcccc atcggctgcc tcaccgcaaa
    11641 acaagctcgt aaaccgcctc cagtacgcca gcggatcgtg ataaaccgct cgctcagcgt
    11701 tccggtctcc cggaatgtcc gataagccca gctcccctcc agcaaggtat gtggctccag
    11761 tacggtccga agcactgaag tcgcgaattt ccggggcttt tttgggggac gtgaatcttc
    11821 gccaaaacgg catcggacgg gtttccgtct cgcgcaaggg tacctgtgaa cggcgctgtt
    11881 gctgagttcg tgacagcctg ggcggatggc gcggctgcct tatggtttcg ccgctcgctt
    11941 gcggtcgagg ttctcaaaga ggcccctgag ccgtcacgga cccctgtgtt ggcctgccca
    12001 aaccaagcga cagccgccat cgtatgatgt aagatacgtg ggcttcgcgc tcgtcgtccc
    12061 gacggcgaag aagccgagct ggaacggacc atcgagacct ccaaggctgc ggggaatcgc
    12121 ttccagcagg tcagcgaccc aagccggcgg ggccgacctt gtcgagtggc accaagctgg
    12181 ccggcggctg atcagacctt cgggccggcg agagtagtcg gccgtgatga ccaatagcca
    12241 gggagacaaa ccgcggatat ggcacacgat cacctcaaag actcgatcga agcacaaacc
    12301 ggacgatcca cgattctgct catcgtaact ctgatgggcg ggttgctggt catcaattcc
    12361 ttcgttgcgg aatggatatt ccccgacaag cagtacagcg cgattttcgc cctgattggg
    12421 gcgatctgtc tgggggcgcc gctggcgttc cactcgctta aacacatcgg gcacggcaac
    12481 ctgccgatgg aactgctggt caccctggcc atcggcgctg cattgagcat tgagaagtac
    12541 caggaggcgg gggtcatcgc tttcttcatg gtcatcgcga cgctcatcga gacccgcacc
    12601 gctttgggtg cccgagcttc gatcgaatca ctcatccggc tgacgccgac caaggctcat
    12661 cggattctgc cggacggaac cgaggaggag gtcgaggcca gactgctgaa ggccggcgat
    12721 ctgatccggg tgcggcccgg ggataacatt gccgcagatg gcgtcgtggt ggcgggtgaa
    12781 tcgaccatca accaggcgac gatcacgggt gagtcgatgc ctgtggacaa gaccgtcaac
    12841 gacgaggtct tcaacggcac cctgaacttg accggctcgc tcgacatccg cgtgacgcgg
    12901 gcgggttcgg agacgacact gggccgggtg cagaagctga tcatgaatgc cgagcagaca
    12961 cgcatcccgc tcatgcggat cattgaccag tacgccaact ggtacacacc gacgattatc
    13021 atgctggctt tcctggtgtg gttctttatc cgtggaccgg aaggcatgtc gcgggccatc
    13081 gccatgctgg ttgtggcgtg tccttgtgct ctggttctgg ctacccccac ggcaatggtg
    13141 gcggctctct cctgcgctgc tcggctgggg atcctggtca aggacgtttc aacgctggaa
    13201 ggggcccgca acctgaccgc gatcatgttt gacaagaccg gcacgctgac gaccggcgaa
    13261 ctgacggtca cgcagatgaa accagcaccg ggcgtggacg gggcgacgct gcttaagctg
    13321 gcagcctcgg cagactcgtt atccaagcac ccgaacgcca aggcagtgat ggccgtcgcc
    13381 cagaaggccc ggttgtcgct ggtccggccg gagcaattcg aggaggtgac cggcaagggc
    13441 gtggccgcaa gaatcaacgg cgacgagatc ctcgtggggc gcaacacctt cctggccgat
    13501 cgcggcgtcg acctgggcct gctgaacgac cccaatttca aggagccgga aggcatcagc
    13561 gttttgtacg tcagccgcaa cgggcgactg atcggctgga tcggccttga ggaccggacc
    13621 cgcgaggaag ccaaggaggc gatcgccgaa ttacgggatc tgggcgtgcg agccattacc
    13681 atggtcaccg gcgacaagtg gtcggtggcc cgacgagtag ccggggaaat ggggtgcacc
    13741 gacgtttatg ccgaggtgtt gccttctcac aagctcgagc tggtcgacca gatgaagcgc
    13801 aagggacacc gcgtggcagt catcggtgac ggagtcaacg acgctccggc actggcggcc
    13861 ggcgacctga gtgtggccat gggagcggcg ggcagcgacg tggccatcaa ctccgccagt
    13921 atcgctttga tgaacaacga tcttcgccgg ttgcctttcc tggttcgcct gtcacgagcg
    13981 gccaccagag tcatctggca aaacatgctg ctcggtataa cctacatcgt ggttttggaa
    14041 accctcgggg ccctcggcaa gatctcgccg atgttggcgg cgtttttgca tgtggtggcc
    14101 agcgcgatcg tgattttcaa ctccgcgcgt ctggttcggt ttggcgaaca tctgcagacg
    14161 accgaacccc cctcggccga gcgaccgccg gtcaaggcat acggcggtgc gcgttcggca
    14221 ccctcggcag cccccatgcc ggcatgatcg atggtttatc ggcctgtgcc cgttgcgtca
    14281 ttgttgtgtg aagcccggcc aagccgtccg atatgggttc gctcgcgctc atcagcccga
    14341 ggagtctttc ccgcgttctc attggtgtag agcagccccc agtgaaccga tagcaacttc
    14401 ggaggcgggc ttgccgcgcg cccgccatcg agtcgggaac ctggtcgtca aagcgggcat
    14461 tataggaacc gccgagccaa gtacatggat ctagcgacct taataggatt ggttgccgcg
    14521 atcggcatgg tggtctggag cctgtgggtc ggaaccagcg gccaaatcag tgtttactgg
    14581 gatactccgt cggccattct ggtccttggc ggaaccgtgt ttgttctcct gacgacccag
    14641 acggtagagc gcttcctgcg acttcacaag gtgtacatgc ggggattctt cgggaaggcc
    14701 atgaccatcc cggaaaccat cgaaaagctt gtgacgctcg gggagcaggc ccgccgcgag
    14761 ggggtattgt cactcgaaaa cgccctcaac gagatcgaag acgagtttct caagaacggg
    14821 atcaggctgg tgatcgacgg cacggccgcc ggcgagatcg aacagattct cacttccgag
    14881 atggaagcca tggaccagcg gcacaatcag ggcaagagca tcattgatat ggccgccaag
    14941 tatgctccgg ccttcggcat gatcgggaca ctgatggggc tggttgccat gctgcgaaac
    15001 atggacgatc ccaaggcgat cgggccaggc atggcggtcg cgattctgac cacgttttac
    15061 ggggcgatca ttgccaatgt gctctgccta ccgctttccg acaagctgtc ctatcgaaat
    15121 gaggaggaga tgctgttgag aacggtaatg atgaaaggca tcttgtcgtt gcaggccggc
    15181 gacaacccgc gaatcaccca ggccaagctg gcggtctatc ttcccgtgga caagcgggcg
    15241 ttgatcaacg gcgtcgaggg taagaaacgg cagtagcggg gtcaaccgct cagcaggaac
    15301 gcgccatggc tctctcgaag aaacctgcgg ccgaaggcgc tccggcgtgg gtacttacct
    15361 acggcgacat gatgtcgctg ttgctgtgtt tcttcgttct gctggcagcc ttcgccgact
    15421 actctccggg gggcggcacg ccgacgcccg agttgaaaaa agcacttgaa tccatccgtg
    15481 ccgccttcgg agcggggggc acgatcggcc cgaacgatga gatcgactac caggcgttga
    15541 tcaagaagct ggaggccata tcgtcgaagg aaacgggtgg ttaccggggt gacacgcgag
    15601 aggaaggcat ctacggcaag catttccggc tccggaagat tcgcgacggc atggagatca
    15661 ccatgggcgg tccggtggtg ttcgagccgt tctcggacaa gctgaccccg gagggcaagc
    15721 aagccctgga acagatcgcc gaaacggtcc ggggccaccg caacaagatg gagatccgag
    15781 gccacgcggc ggaggagcct cggccggcgg actggaccga tgatgacgct ctgcagctct
    15841 catttcgaag ggccaaggca gtggctgatg agcttgtcct gcgcggcgtg gaccggcggg
    15901 cactggtctg ctcggccgtg ggagccaacg agccgttggc aacaggcgtc tacgacgtga
    15961 acaggcgggc ccaggcgcgc cgcgttgaaa tcatcgttcg tgaatcgctg atcgacgatt
    16021 acgtcaaacc gtcgagcgaa caagacgctc gcgccaagat actggccacg ccgcccacca
    16081 gcacaccctc gacgactccc agcgctccgg ccagtcaaga ctcgccctga cgcctaatcg
    16141 tgcgtcgggg ccccggccgg ttcggccatc ggctcctctc gcacttcgta gcccggcagg
    16201 ctgggaagat tgacctcgct ggcggccatg cctttgttca gtcggacgtt ggagaacgag
    16261 gcaacgatct cctgcttgtc ctccttagcc accgtcgcaa cccgaaccgg tacgtccagc
    16321 gtccggtcga tgtggaagtg gactttgccg tagcggcgat ccagttccgt tcccggcaac
    16381 ggtgtgcatt ccaggtgatc cgagttcggc gggtctgtcg ggccgggagc aacaagcttg
    16441 atggtgaagt gacgaatgat gtcacctttc ttctggccga agggcatcgg gaaaggcccc
    16501 ttgccaattc ggaagagatc ttcggccttc tcgcccggtc ccacgacttc tcgcttgatc
    16561 acctgtttcg accgctcacg gacctcgatg taccaccgac cgtcaaagac atgccactcc
    16621 ttctctttct cgaccacccc ttcgacgatg cactcatcga aacgaatgaa gaatcgcggg
    16681 ctgggcttgt cttcgatgaa acgaagaaca cccttgtact tctgctcgta atcgattaaa
    16741 gggtctttct tgatatacac cagatcggcc tgcacatcgc gcacatcctt gttctccagc
    16801 cggtcgagga tcttctcgac cgccggatcc aacggcgcgg gttgagaggc gggctcagtt
    16861 gccgggcggc tcgccgaagg cccctcctcg cggcttgcct gcccgccgga ggcaccgcag
    16921 atcaaacaaa ccgccgagaa gcacgcgatg aatcgtacac ccatcgtcac aatcctttca
    16981 gaaccaagat ctcgtggcga cacacgcgca ccggccgcgc gctcatcccc cagaattgta
    17041 aataccgagt ggcggggtaa caagatccgg gtgcaccccg ttttcggtgg cgttacgaag
    17101 gtagggtatg gtcagaatcc gcgcagcgca tgagacctgc cgacgattct gtcttacccg
    17161 ccgcaaccat cgaccgatgt cgccaccgtt tcagaaacat gcccataaga ctcggatcac
    17221 cgaatgagtc cgtgacagaa gcggcggcac tcctcgcggc tgacccggtt gtcgtgaacg
    17281 caagccagac cgcttcagcg gtctttcccg aagggcaaag aggccgcctt tggcgggcaa
    17341 acccgctgaa gggccttgat cgcccaacct ggaccggtcg gcatgcccgc cgagactgtc
    17401 acgcaccctc accgaatcgc ccgcggaccc tgtcggcaag gtcagacagt ctgagcttcc
    17461 gcgcccgttg ttcacgaggt accatctatc ggcagcacac cactggggct tgccggccag
    17521 tgcgcaattg acctaagcgg accgcctttt tgcacgccgc agctcaagcg cactgcgatc
    17581 cagaagcatt cgagcaggcc gcacggttgt cgggccgtaa ggttctgtta gaggcgacac
    17641 cggcatcagt cgaaaggaag ccaatggagt cgcgcagtct tcaaccacta cacggtcgga
    17701 cggtcgcggt cacccgcatg cgtgatcaag cggacactct cggcaggttc ctgcggtcgg
    17761 ccggcgcgga ggtgatcgag gccccgacca tcgaactcgg tccggtcgag gactaccgca
    17821 aggtggatga cgccctgcga catctcgatc gctaccagtg gctggtgctc accagcgcca
    17881 acggcgtcga cgcgatgtgt gaccggatca gggccatcgg gctcgacacc cgctgcctgg
    17941 ctcgggtgaa gatcgccgcg atcggctctg ccacggcccg gcggctgacc gaacaaggtg
    18001 cgcggccaga cctcgtgccc cccgaagcgg tcggtgaatc aatggcccag gcactggtcg
    18061 aacagggagt gcggggcagc cgcatcttgc tgcttgtggc cgacatcgcc cggacgcaac
    18121 tgcccgacgc gctgcaagcc gccggtgccc agtgcgacag tctggccatc taccggacca
    18181 aacggcctgc ggccctgcct gcggtcttcg ttgagcgact cgatcgcggc cagattgact
    18241 ggatcacgct gaccagcccg tcgtcgttcg tcaacctgtt gtggttgctt gggccgcagc
    18301 actgtgagcg gttgcacacc gtgggccttg ccagcatcgg tccggtcacc acacgggcca
    18361 tccgcgaggc cgggtattcg gtggccgccg aggccgaccc acatgatatt cccggcttgg
    18421 tctcggccat tatcgacgcc tcaacgcgag actgaaaccc gtttgagttc gtcccagcct
    18481 gtcgggaccc ctcccacccg tctttgagct ttgatctttt cctgctgcag gccctcgccc
    18541 atggctcaac gcctcggtta gaatgtggcg gtcgctcgtc aacccgagtg ccatctgtac
    18601 gttgaggaga acgtatgagt ctgcaatacc gggtcatcag tattgggacg ctgagccgaa
    18661 accgtttctg gggcgagacc gagcccaagc gatttccaca cgccaccaca acgctgatcc
    18721 gggcggacaa tgaaacgatc ctcgtggacc ctagccttcc ggcggaagtg ctggcacagc
    18781 ggctggagga gcgatcaggt ctcaagcctg agcagatcca ggcggttttc ctgaccacat
    18841 tccgccccat acatcgacgc tccctgtcgc tcttctccgg agccacgtgg ctcatgtacc
    18901 ggcccgagat cgaggcagta caggcacacc tgaacgaaat gcgcgaacga gcccaggagg
    18961 gacccgagga cccggaactc tgcaagctgc tggatgagga ggaatctctt gtggctcgta
    19021 tcgaggcggc cccggaacac ctgacagccc agactcatct ttttcccgcg gtcggtgcaa
    19081 cgccgggatc agcagctttg ctgcttgcct tgccgacgca aaccgccgtc atcaccggag
    19141 atgcgatcgt cactcaggaa cacttcgcgg ccgggcaggt atacgagcat gtggccatga
    19201 tagaagctgc ccgcaagtcg ttcgaagaca ttctagaagt ggccgacgag gtggttcccg
    19261 gtcacgacaa tgtctttcgc atcggtggcc tttagtcccc ccagaggcgt ttgtcaagca
    19321 caggggccat ctggcaggaa aggcgtcggc aggtcacgaa gccgcacggt atagctgtcc
    19381 ttgacaccca cggaagggct gggtagtctc gactgcgatg atcggagcgt gagagatgga
    19441 agtccgcatg gacctcgcca ggatcgtgat cagcgatacg agcgagcagc aaatcattgt
    19501 cctgcaggaa cacgagggct ctcgccaatt cccgatcgtc atcgggctgg cggaagcttt
    19561 ggccatcgat cgccgggtca agaacatcag cacgccacgg ccgctgacgc acgacctgtt
    19621 ggccaacgtg attcgcgaac tgaatgggga actggaaaag atcgtcatcc atgatctccg
    19681 ggaccacatt tttttcgcga agcttgtgat ccgaagcaac ggcaagctga tcgaggtcga
    19741 ttcccggccg tcagacgcga ttgcccttgg cgtggcgagc gatgcggcca tctacgtgac
    19801 agaaaacgtc ctccgtcagg tgtgtgagtc tcccgaagcc tgaggaggag aaggccctgc
    19861 gaacgctgaa gaggtagcag tgagcattac gggaaccgag aacggcttgt cgacccggac
    19921 agatcagttt ctccaactgt gcgaacagcg ctggccggct catcagccgc gacccgccgt
    19981 tgtgacgcgc gctcctggcc ggctggattg catgggcggt atggccgatt tcagcggtgc
    20041 gctggccctg cagacgacca tcgaacaggg ggtttacgca gccgccggtc gccgcgacga
    20101 ccagcgaatt cgggttgagt cggtgggctg gaatcaagac ggacagcctt ccgtcttcga
    20161 gtggtctttg tcctggtttt atcagtccga cggccaattc gtcaccgcag agacactctc
    20221 gtcgaagttc gatgcctgtc catgggcccg tcacgttgcg ggcgtctgtc tggctttact
    20281 ggaatcaggc gatcttccgc atctggccgg cggggtcaat ttcctgatcc aatccgatat
    20341 tccgcctaag gcgggcttgg cttcgtcggc ggctctgcaa gtggcgtgcg ccaaggcttt
    20401 ggctgctctg ttcgacctgg aactgacggc acagcagttg gtgcgggcct gccaactggc
    20461 gaacaccgag gtagtcaaga atgagtccgg cctggtggat catgtgacct gcctcttggg
    20521 cgagcctgat tccctgctgg agattcgctg ccagcctcag gaggtcatcg gatcgctggc
    20581 gatgcccaag tgcgcggcct ttgcgggtgt gtattcgggt ttgaggcttc ccttttacca
    20641 ggaacgctat gccgacaacc gggctgcctc cctgatgggc aggtacatca tcgagcagat
    20701 cctgcggacc tcgggggatc cgggtgagct taacagcgcc tacctggcga gtattccgcc
    20761 caacgaatac gttcgacgat tccgcaacta tttgccgccc aagcttcgtg gtcgtgattt
    20821 cctggaccac ttcggacagc ccccggagtt ggacatcatt atccggcccg ggcaggtata
    20881 caaggtccgc tcccgaacgg agcaccacat ctacgagaac gaccgtgcgc accgtttcat
    20941 ggaacggctg gcccgcgtct accgcaccgg cgacctggac accctaatag aggccggcga
    21001 actgatgtat gcctcccact ggagttacgg ccagcgatgc gggatgggaa gcattgaaac
    21061 agacgtcctg gtcaattgca tccgcgagcg cggtccggcc tgtggccttt acggagccaa
    21121 ggtcaccggc ggcggctgcg gtggaacagt ggccgtcctg atggccgaca catcggcggc
    21181 cctgcaggca ctttccgatg catgtgccgc atacgcggcg aagacgggca ggcagcccat
    21241 gttgctgagc ggcagttcgc cgggggcaat ggcgttcggc caccggaaca tcggttgacc
    21301 ggccttgcga agaccggcga tttccagtga ctacagaact tgacggccgg aagtctgtgc
    21361 gtcttggttc cttccgtcgc acccgacgtg cattttgcgc ccatttttcc ggccccgccc
    21421 agccgcttcg attgcggcct tcaatcccca tcggacgccc ctcgtcctcc gagcggatgc
    21481 tctggcccca agtccccagc cctgctgctt tggtcatgga tcgggatcac agtgcgcttt
    21541 ggctgccgcc tgtaagaagt ggttcgcctt cgatcaccgg cgcttgccgc cctgaagcga
    21601 tctgacgtcc ttgcccggcg ccgatgcccc cggtttcctg tgacatttcg tccggtctta
    21661 tcctggtgca gacgaggtgg ccggctcgcg acgatcgatc tccaccttga gggccacgag
    21721 attccgctcg ccctctctct tttccacgcg gccgagcacc tttacccgat cgccgattcg
    21781 cacgtcatca aggctggcgg tcacgccgtc gatgaagatc tccgcctcgg gagaaagccg
    21841 tccgtgaatc tcaaccggct tgcggtgttt ctcgctgaag taggacatcg agacctcacc
    21901 gctggtcttc tcgatcctgg tgaccttccc ggaaatctgg tcgtattcgg gtaattccga
    21961 atcctctccg catccgccag ccaggagagc accgaagagc aaccagaagg caaagatgac
    22021 gacaattgag gatctcgtgg tcataggggc accttctcag tatcgtcagg caagcttcaa
    22081 tcgatgggcc gtctgaagcc ggccggtgtt gccgatgttc gtatacctca tctggccttg
    22141 ccttggcaag ttctgaccct ctcttctggc gatccttcat ggaccggatc ctgatcggcc
    22201 atcggctcgc ccgcttgtgg catccctgcc gcctaacgcc tataacgtac tcgattgggc
    22261 tggaggaaac gagcatggcg gatgggcctt ctcaggtgga agagatgttc ggggtcggca
    22321 aggcccttgt ggggatggtt cacgtgggcg cattgcccgg cacgccgctg ttctcgcggc
    22381 cgatgccggc gatcatcgag caggccgtca gcgaggctcg actgctggcc gacgcgggct
    22441 ttgacggcat catgattgaa aacatgcatg accggccgta cctgcgccag agggtcgggc
    22501 cggagatcgt cgcggccatg accgccgttc tcgacgccct gcgcgcggcg gtcagcaaac
    22561 cgttgggtct tcagattctg gccggggcta accgcgaagc tctggcggtc gcggccgccg
    22621 ccggggcttc tttcattcgc gttgaaaact tcgttttcgc ccacgttgcc gacgagggcc
    22681 tcatgtccga ggctgatgca ggcccattgc ttcgctaccg gcgagagatt ggtgccgagc
    22741 acattcgcat cttcgccgat gtgaagaaaa agcactccgg ccatgcaatc accgccgacg
    22801 tgaacatcgc gcaggcggcc cgggcggcgg ccttcttcgg ggcggacgcc atcgtggtca
    22861 ccggcccgat gacgggcgag gccgcgacag tcggggacgt ccgggaagtg gctgctgccg
    22921 tggaaatccc cgtggccatc ggctcaggca tcacccccga caacctgccg gtcttctggg
    22981 acctggcgga tgtgtttatc gtgggctctt tcatcaagca ggacgggcga tggtatcacc
    23041 cgcccgatcc tgaccgggtc aacgccctca ttcgggccgt caaccagctc cggcgagtcc
    23101 aaaggtccgg actctgaaga atcggctggc cataggggaa gtggaggact agaatacggg
    23161 cacaaaacat gggaggcgtg ggcctgccca gtatgaggtg aattcaccgc ggagagacgc
    23221 tcatgacaag attgctgagg ttgattatgt cggttggcat agtggtcgcc ctggccggtt
    23281 gtgcggctac cgatggcggc agcacgcaga ggcaggcgag cgagcaaccc ggcgtggcaa
    23341 aggtctcgtc agagaccggc ggcgagaagg cgggctgtcc gaagaccaag tgctgcggtg
    23401 agaaagccgc cggctgttgc cacaagccct gcgagaagaa gtaggcttct acaggtcagc
    23461 actgcattcg agcgacgctc acgcgagctg cttcggcggc tcctgatact tgcgctggca
    23521 acctaccgta atcagcagca tcggttgaaa aaggggcggg cactcggcca tgcaggcccg
    23581 cccactctta tgcgcatgcc gtcctcgatc aggtcatcag ccgtcgtttg aggcttgcca
    23641 gttcatcccg caaggcatgc ggcagtcggt cgttgaaaag cttgtagaac tcctcgatgt
    23701 cctgtgtttc cttgagccac gcctcgcgat cgacggtcag cagctcctgc aagacctctt
    23761 gtgtcaagtt caggccgccc aagtcgagcg aatcgggcgt gggcacgtaa ccgataggcg
    23821 tggcggttgc ttccgcctcg ccccgacacc tggctaggac ccactcgagt acgcggagat
    23881 tctcgccata accgggccag aggtactggc cgtccacgtc ctgtcggaac cagttgacgt
    23941 ggaagatctt gggcggcttg gtcatctgct ggcccatctg cagccagtgc atgaagtact
    24001 cggccatgtt gtagccacag aaaggcagca tggccatggg gtcgcggcgc acctcgcctt
    24061 gtttgccgta ctgggcagcc gtccgctccg aagccatggt cgacccgatg aagacgccgt
    24121 gctgccaact gtaggattgg tagaccagcg gcgccagtcg ggcccgccgc cctccgaaaa
    24181 tgatggcgga aatcggtacg ccgtggtggt gctccgttcg gaagctgtgc gatggacact
    24241 gcgacagcgg cgcggtaaag cgagcgttag gatgcgcccc gagaacgggc ttgccgttcg
    24301 cgtcccgcat atcgggtgtc cacggatcac ccttccagtc gattcccttg gcggggggct
    24361 cgccatcgcc gtcctcccac cagaccgtct tgttgtcggt cagcagaacg ttggtgaaga
    24421 tcgtgttgcg ccggaccgta gccatggcgt tggggttggt tttcgtattc gtccccggcg
    24481 ccacgccgaa catacctgtt tcgggattga cgccccagag cgcaccgtcg gtgtctattc
    24541 gcatccaggc aatgtcgtca cccacggtcc agatccggta acccttgcgg gtcatgcttt
    24601 cagggggaac aagcattgcc aagttggtct tgccgcaggc actcggaaaa gcggcggcga
    24661 cgtactcgat ccgtccggac ggatcttcga tgcccagaat cagcatgtgc tcggccagcc
    24721 agccttcctt gcgggcggca cagcttgcca aacgaagggc catgcacttc ttgctgagca
    24781 gcacgttgcc cccgtagccc gagccgacgc tccagatggt attgtcctgc gggaagtgca
    24841 gaatcagacg gcgtttgtca tcgaggtccg ccttgccgtg caagcatctg gtaaactcgc
    24901 cgtcggcgcc caacttgtcc aagacggcct gcccgcagcg gaccatgatc agcatattga
    24961 gtacgacgta gatgctgtcg gtgagttcga caccgtactt ggagaagacc gacccgaccg
    25021 gtccgagcga gaagggaatg acatacatgg tccgtccctt catcgagccc ttgaagatct
    25081 cggcggctcg gcgatatccc tcttctggag gcatccaatt gttggtgggc ccggcctcct
    25141 ccttcctggg cgtgcagatg tacgtgaggt gttccgttcg ggcgacatca ttcttggccg
    25201 tgcggtgata atagcatccg gggtacttct tctggttaag cttgatcagc tcgccgctgg
    25261 ctacggcctc ggccgcaagc tgctcgcgct gtgcgtcgct gccgtcgatc cagacgatct
    25321 tgtccggccg gcacatggct gccatttcct gcacccaagc gttgagtttt gcgtgattgg
    25381 tcatgtcgcc tcacctcttt cggtattccc gacgggtcct tctccttcac gttgtccggg
    25441 gaccattcgt gttctggtga cggcacgttg gtccctgcgg acgcgccgtc acgacgcccc
    25501 ggcccggggt gacaggcgca caagtacccc tggccgaaat cgaccctttt gtcgcctgat
    25561 gaaccctgtt aggatcccgg aatcacgcca aaccggtggt acacgcggct ggcagaccga
    25621 tgggaggccg gtcccgatct ctcgtcggac cggctcacta gtgcctcagg gcggtccgaa
    25681 agtccacagg cagaatctca tgctatccgc ccggcgggag cgccgcaagt cggcttcgac
    25741 ggtgccctcg tcggtcaagg caggtctgcc agagctgtgc gggccgtatc acgcctggat
    25801 ccacaggggc gccggctggc ccggacaacc gagcgtcggg taaaatcccg tcttgagcac
    25861 gtgaaggccg caaagacaac ccatggggct ccggagggca cctggccatg tacagccggt
    25921 ccgaaaacgc cgcaacaatc gttcggggaa acgcaagggg gtctctgtta gaggcagcct
    25981 tgctgctggc tatcggattc tacttgcgat ggagcggcct cgaggggatc agtcgctcac
    26041 aactgtacaa ctggtctgtc gacgtggtgg tctggacatt cctgataggg ggcttcctga
    26101 tcctgggggt tatgcttctc tgctggacgg gccggccgtg ggcattgatc gtggatggac
    26161 tagtcagtct cctgattggg gccgcgctgg tgggttgcgg catcgtctgg ctgatccact
    26221 cggacatcca gggtatcctc atcttgctct ctggcctcct tgtcctcagt gccgccaagg
    26281 ggagctgggc caactaccgc ctggccctgc aggcagtgac aacggtccct tccggagaac
    26341 cttccttggt gaggccggtg gaagaggcgg tgcctgctgt accagaccct caagccaagc
    26401 cgaaaccatg gagcgattgc tgtcgaccaa gcaggctcga ccggccgttc ctgccaaggt
    26461 ggatatcgct cgcaaggacg agccggtccc cgacggattc cttgcccagc ttggcaggga
    26521 gcaggacccc aagggcgaat aagcatggct tcgcgtgatt tgacgcccga ttcatgctaa
    26581 cattttcccc cgtttcgccc cggagcgcgt gtgaaaaggg taaacaacgc ggcaggtctc
    26641 ggtcgttgcg gcgacctcga ctctgtcgtc caacatggct ccgttgccct ttgcaccggc
    26701 tcctgattta ctgggatgaa ctaagctggg tgtgtgagat catgcacaac gacaccatca
    26761 ccgcaccggc cttgaaaccc gccggagcgg tgatggtgtc gttgtgcatg atcaaacagg
    26821 tcaacaagct cg
//
LOCUS       DAOTPW010000046        26754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1889_length_26754_cov_5.566426, whole genome shotgun sequence.
ACCESSION   DAOTPW010000046 DAOTPW010000000
VERSION     DAOTPW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26754
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_26754_cov_5.566426"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(749..1642)
                     /locus_tag="P5316_06140"
     CDS             complement(749..1642)
                     /locus_tag="P5316_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR84574.1"
                     /translation="MADLVQRRTGARGRQASWGRSAFTLIEVLVVVAIIALLVAILLP
                     SLSKAREQSRRAVCAAQQREFAKGTLMYLLDSRDVLPGPIHGAMELETAGKVASKDYE
                     EWHLPHFIRRYFHDRGRQGRLTDEVASCPTAYTLTAASYQKAFAEADFRRPFTYALNN
                     WNRNHSTQVEYGTNPPWYFGYPDHYWNNTKPPFTPLASPTVLTKDAAPKKISLIRQPA
                     REWAFGDAFRWDPQAPLELARTLKPGQWKKGTYQLDFVHENGLTLIPKGPCHSGGINV
                     TMFDGHVEWQRPWFGTLNPQK"
     gene            complement(1868..2914)
                     /gene="hemE"
                     /locus_tag="P5316_06145"
     CDS             complement(1868..2914)
                     /gene="hemE"
                     /locus_tag="P5316_06145"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRR84575.1"
                     /translation="MDCEVLHNSPFLRACRREKADYTPIWLMRQAGRYQRQYREIREK
                     VSFLELCKTPGLAAQVTVMAVEQLGVDAAIIFADILLIVEPMGVGLSFNKGEGPSIAR
                     PVRSGVDVNQLREVDVDDSLSFVFEAIRIARRELAPGIPLIGFCGAPFTVASYMIEGG
                     ASREFTRTRSLMHDDPGAWNALLERIVRASADYVNGQIAAGAQAVQVFDSWVGCLDEQ
                     EYRRFVLPHTQSLIRAVSAGTPVIHFGVNTGSMLKAIREAGGDVIGLDWRVNLAEAWK
                     TIGYDVGVQGNLNPNVLLTSADEIRRQAKIILDQAAGRPGHIFNLGHGVLPNTPVENA
                     RALVDAVHEYSRGF"
     gene            complement(3045..4094)
                     /locus_tag="P5316_06150"
     CDS             complement(3045..4094)
                     /locus_tag="P5316_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84576.1"
                     /translation="MGKTYGFGVVGAGMIGKFHAEAIKGLPNARLLAICDQVASAAET
                     LAGKFGCGAETSLEKLLSRDDIDVITVATPSGLHGEVAIAAARHGKHCVVEKPIEITL
                     EKIDRILEAHDKAGTTVGGIFNMRHEETAKLFKKAVDAGRFGRMTFGMAYGPWWREQS
                     YYDNGGWRGTWALDGGGALMNQGIHTIDTLQWLMGPVKAVTAFCRTLAHERIEVEDTG
                     VAAIEFANGAIGTIACATSMWPGHFRIIEVAGDRGTAAMADNKFFFWQFAQETPEDAR
                     IREKYLQFPGVSVGAANPSAGMTADNHRANFAEFLAALDAGKEPPISGREARKAVEII
                     LAIYESSRSGRTVSL"
     gene            complement(4522..5115)
                     /locus_tag="P5316_06155"
     CDS             complement(4522..5115)
                     /locus_tag="P5316_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84577.1"
                     /translation="MRLPELESPEKYRGLYVFDFGDQVAVGYTADEIAVLLESQRYAD
                     GKVYRIHRAMPDGTMELQGVSRETFLKEDGLFFYRDEPGLAEEDFQTLVREAAGTPPP
                     CRMKVHLARISGSRYELCTVAIFPAEYTHDVSDWLNRIGYEGGDLVEGGPSQVSDYYA
                     AGATVIKRQQLWPMPSQSRSADEVLATTHLAVQRKMA"
     gene            complement(5425..6441)
                     /gene="rtcA"
                     /locus_tag="P5316_06160"
     CDS             complement(5425..6441)
                     /gene="rtcA"
                     /locus_tag="P5316_06160"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HRR84578.1"
                     /translation="MLTIDGSFGEGGGQILRSSLALAMVTGRAFRIEKIRAGRKKPGL
                     MRQHLTAVQAAAQISQADVRGADIGSKVVEFHPKQVQPGEYSFSIGTAGSTTLVLQTV
                     LPALLTASGPSRLTFEGGTHNPFAPPFDFLAKAFIPLVNRMGPTVSAELERPGFYPAG
                     GGKFTISIEPARELKGFELTERGEIIRRCGRAVVSNLPGHIAEREINVLRKGTNWGES
                     CYSVENVAHPRGPGNVVIIEVEAENVAEVFTGFGALGRAAEAVATHALQAYQRWLKAD
                     VPVGEYLADQIMLPLAIAGRGRYRTTPLTLHSTTHMDLIQKFLDIEVAVDHLDRNRCE
                     VRIG"
     gene            complement(6669..8261)
                     /locus_tag="P5316_06165"
     CDS             complement(6669..8261)
                     /locus_tag="P5316_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HRR84579.1"
                     /translation="MQFDSHDINRDSGSKGGACCGHPDERAAGISRRSLLGGIGGAAA
                     VGGLAMLAASRAQAAEAGQSAGSGLPMGAPLKVKPALMYSIPQRREKTSWRPYGGILT
                     EEDLAAEVARVEQDLAKLSSQAEFRLEMQPPAMVAGPDQAKSVASSDADAIVLFAAGG
                     DQAWLETIAASGKPVVMFVRHKSGPVYLYYEIVHWRWLRKNEDLIKETRIGVDDIVVD
                     DAGEILWRLRALHGLKNAKGTRCVAIGGLRAYSVPGQQKGPDHVKQVWGYELITVPDE
                     DVAEQLAKARKDESAMKEIRRQADEFLAQPNVDSSIEKRFVENSFLALRIFKEIMKAN
                     GAANLGVAHCMGSLIGLLDTAPCLVLSMLNDEGFTAFCHTDYTHTAPGVLMRWISGKP
                     SFVCNTHFPHDGVITLAHCAAPRRMNGRDYAPTKIMTHFESDYGTATKVEYDKGQVVT
                     VIIPNLNCTKWFGFRGRIVDSPHYDMCRSQMDVAIDGDWRRMAREMQGFHAVVTYGDY
                     LREVGYVLGKVGIEWQDFSEKA"
     gene            8574..9884
                     /locus_tag="P5316_06170"
     CDS             8574..9884
                     /locus_tag="P5316_06170"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84580.1"
                     /translation="MKRRDFLRKSAAAAAAGVSFPYFVPRHVLAAPGRPGANDRFKVG
                     FIGCGGRAHALMNTERLSDHADIVAVADCFLTRIDERAKATPDADKWVRYQDYRKMLE
                     KEKLDAVFVPTTTHARVLACITAMEAGCDVYAEKPFALTIAEGRSLVKAVKKYKKVLQ
                     VGTQQRTIPINAWASKLIRDGGIGKLKEVQAPNFIGPDRWKPLPAEEIPQGLDWDLWC
                     NQTELRPYHKELHYGWAKWWDYDGGGKSWGVTGWGTHAFDQAQCGMGADDTGPIEIWP
                     EGADAAGNVKVTMKYANGTLLKCHNPLRLKDDKGNNVYHDLGAIFVGEKATLEIRRGS
                     LVCDHPELIKDAPPGYPAATNGEVVWHIKDFFDCCRSRKQPAAHVEAAQRATTLCYLV
                     NICRDMNRKLKWDPNAERFVGDDEANRELSRPRRKGYELPKEFT"
     gene            complement(9922..10584)
                     /locus_tag="P5316_06175"
     CDS             complement(9922..10584)
                     /locus_tag="P5316_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR84581.1"
                     /translation="MALQRLILFPGMACDERLFSPQSSFGVRFECPRLPVPRDGEDLP
                     SYAARVVSDLGLGRTPCVLGGVSFGGMLACEVAARCNCKRVILIASCRSSAAIPKRNW
                     LIFHPAKLVPDTVIRGISGPVSRVVARMEGIGHEHHRLLLDMCRDVPIVQLRRQAAMI
                     LGWRNPSRLDCPVHQIHGRRDHMIPLGGVQAEQSIADGGHFINLTHPAAVARFIAQHS
                     AQ"
     gene            10877..14362
                     /locus_tag="P5316_06180"
     CDS             10877..14362
                     /locus_tag="P5316_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRR84582.1"
                     /translation="MFMQRLLSRTVLAVTVISLLCPMLMGPGCPNLEMIPLNNDPEAL
                     QPPDDSSGSSTPATLQIYAGPDREATVGSPITLSATVEGGEEPYLASWSPASALSDAN
                     TLSPTFVPPAEGEYEFTLTVADRKGLGGSRTVKVNALDHATLASLKWAPNFSDGGYQL
                     IAAFTKPLDKPTAENVNNYRTTPKADDTRAASPREIVAKVSVKPTSATLSSDGLVVTL
                     LFDSTTLARNTRFDISVSNGILGADGVAVSEIKGLPAAADSADTKAPTVVSRRWVTGT
                     SVSDAETAAEDKIWENSGVHTEVSYSYILQVVFSETMDPVPAVNPAAYRIQEGTNRYA
                     PAIVELANDGRTATLIFTDGPLSQESKLDVGLTRLRDMNGLDLVLDASKSVAANADDK
                     VAPKIVAESVRFLSSSGEGYRVEITFDEPMDRKTCETAANYLLDGHAASTAELGSDGR
                     RVVLTFEGLAATTNSKLSIPANKVKDINGVSLAAQNNLSILSADDPMTPRGVPTLTWL
                     PGDKTTGYEIWAEFPLIMDRATVEDVRNWRITGTDLNPKSVVLHSTTNAAAGTIAGQT
                     AKITFWDPGLTDQTERTRRARLLNRRTQLDVSVGASIRDISGTAIGQVSIPVVANEND
                     VTGPRLVPLPGQTEAKPIWGDVLKSEYGKTHYSVSLAFSETMDSLSVLNSENYVLGGV
                     RPTAIQLDLDGRTVVLTFAKLTTPLGKSDQLRLYSTLRDINGRANTSVTPMTILPASE
                     TTPPTIESLYWLADSRPYRVVVKFSEVMDRDSVEVVDKWLLLDYGGIDRKPTQAVLNP
                     SEPTTLVLTFENGVFATDSVLSLDGEIHDINGNAYDGSGGGIVLPAAPADDANDVSAA
                     RLLSATWMVDSNLGYLVLVEFSEAIDADHAGTFTLDGIGPAWTMITDGGTLVVALFPD
                     PATPAVFNRDSRIAISGLQDMSGNVSTTMVRNVAANPLDTLGPTPLRAMWAADYGDAP
                     AEQGYQVIVVFSEVLDALTAQVGANYQITGTFSKPTEAALSPTDGRTVVLTFPPTVLY
                     RSLAATDTLDASTGNSILDVNGVFGWQATVDITRNSADTTSPTVVAATAVGGTGVEIL
                     FSEALDRVSAEMLDNYVLTGNVYPTTALLKTDGRTVTLSFDQSVSGKNLRVSAQDTIA
                     DVNGNRLSATDVGPL"
     gene            14598..18332
                     /locus_tag="P5316_06185"
     CDS             14598..18332
                     /locus_tag="P5316_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84583.1"
                     /translation="MARKHVGKRVVLVFWALCLGTPLLMGQGCCEGLWLLGLLGAGQM
                     AEPRCPTLINPLTVDAGDDLAVAAGGNVQFGASVTGGTVPYIFAWSPTTGLSDPSALR
                     PTFTATTAGQFPFTLTVTDAAGCSISDALIVTVSAGDGDSGHGIGGDGGDGGDSGTDG
                     SSQPQPLVVDAGPDRTGTVGASMLLQGSFTGGTGPFTISWSPAGSLIGPNTLTPTFTP
                     TSAGNFTFTLTVTDGTGASESDSMVVSVSAMTSLSSLRWGANFAGESYQLLAEFSKAV
                     DKASAERLTNYCVSGTETHPVSAVLSSDMRTVVLVFADVKLTAKAEFDISVGSGVLDS
                     NGTRIPQVKGLVPAPNTEDTTAPKVALIRWAVNYNGSYGVELIFNEAMDQSTLENARS
                     YEIHDNGVAAVAFDAQMGLDGKTVNLVFYGIPLSQAAKLSVGLLTIRDINGQRLQPVD
                     QQAISANSNDLDPPVVVDNSIQFVVDYSAGGYQVVLDLNEAMDRVSVEDPTAYLIDGL
                     AASAAKLGDNGRHVTLKCANNSYAPTSKLSILGNRVKDINGRYLAARSNLAILPASST
                     GAIPSVPVLTWLKGDESTGYQFLARFNESMNKTTVENKNNWRISGTNIRPTSVVLSTQ
                     TVGDEIAGRTAKVTFGDFGDSRTTMSRTTRVDVSVGDSITDITGQRLAQVTCAIGAHP
                     GDTVSPFVAAPYSGLPARPVWGDILSPDGYRGDTYVVSVVFSETMDATSTMDATNYYL
                     VGVHPTSVVLGPRGREVILTFANIPGGLTSAEKLKILGNVRDINGRRTSSVVSLPIQP
                     SPDDMMPPGVSDMFWGINKGPYEVAVTFDEIMDAESATNVAAYTIDGLNPTAAIMQPD
                     GKTIVLVFGNSLLTPLLELSIVGEVKDLNGNAYDGSHGDVSLPAAPSARPETDRQAAR
                     LASAVWAVDSLDYRILATFTEALNAGTAGNPDYYEAAAGFNRFKATSAVLMPGGITVE
                     TVFADFGNPVPGQLPPSPLNRTAQLNVVQSMGGVTDMHGWLSRTSMIVGANPQDVAAI
                     ELPNPVATWAANFDQGGYRLTVTFAGEVLDARSAEETRNYMISRTFVNPTSARLSGVD
                     DLPRGIYAGRTMFLTFTKKPMSIDTTIEVSVGGSITDMSGNSMPQITQLAINPNPEDT
                     TPPEALHAGPGAQLGQIKVTFSEAMDETSTESPLNYMLLVDPNDPQAPFYLPYAVSLD
                     NDGMTAYVQFDLIPQTGMFLRIAGVQDVNGLTIVEVDLRL"
     gene            complement(18395..19354)
                     /locus_tag="P5316_06190"
     CDS             complement(18395..19354)
                     /locus_tag="P5316_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRR84584.1"
                     /translation="MSDIVNVGICCVDAIGQTIDDYPPPGGLRLFDRLTLTTGGNAVN
                     CSIALAKMGVACDAIVKVGNDALGEFVVAELKKYGVGTKGVIHTSGIHTPFTFASVFT
                     GGQRSFFHTMGANGTLAYEDIDLEIIRGAKFCFVTGTMVMATFDGEQTARLLRDAQGF
                     GAKTLLDTVYLDSAPREQWHRNIYPSLPVLDYFIPSQPEAKAITGLSDPSDIAKAFQD
                     RGCRNVVVKMDEKGAFCRDASGAEDLVPAYKVDRVVDTTGAGDSWCAGFICGLHESLS
                     MPEAALLGNATAAHCIQAPGASTGIVALDRIREFQRATPLRKP"
     gene            complement(19351..21363)
                     /locus_tag="P5316_06195"
     CDS             complement(19351..21363)
                     /locus_tag="P5316_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84585.1"
                     /translation="MCASSTGLILIVGVALVAAIIATESMAALAIAALDGGGTVLVLA
                     AASLAGGWLVHLVGLGRAPWHERLIIGAGLGTGGLSLMVLGLGSAGWLNRPAALALAG
                     ILAAIGIARLALDVRTLQQAGPQPRGQESGRLNWLWLMACPFLAIAILAACLPPGVLW
                     GRPGTEEAYGYDVLEYHLAVPKAFFEQGRIGFLANNTYSNFPLNSEMLSLLMMVLRGD
                     AVEAAFMAQMVNVFFAGLFAAAAWLVGKAFSDRSGLVTGIAALTAPWTAYLAGIAYVE
                     VGMLAMGMCAFAVVLRADVRAEGASRRGLAAGLLAGLSAGFKYTAIPMIAVPAAMLIV
                     FCRRSPQKRLLGMATFAAGFLIAFSPWMLRNLMNTGNPVFPMAYSVFGAKPGLWDTEL
                     EARWQKAHGWQGIAEADKPLFVRLVGRTIGDYRMGGMVVVLAVVGAVVARDRWTVALL
                     VILVLQAVFWTFATHQYARFAVVMLLPLATLAGRAFGGRAGGRGWLLGLLIVGTAWNL
                     YRLAGVYYDHTRVRTMTGDVGLDAYGHTNWFMSGQWPGTRTLKPVNARPPGTVVMLVG
                     EARSYYVVSPCDYATVFNRHPLADAVRDRRDPQEIIHWLRNRGIRYILADWTEMERLR
                     NTYGFDPEIDAALFEKLEPAGLEPIEEFLLPETERVHATLYEVPLR"
     gene            21611..22690
                     /locus_tag="P5316_06200"
     CDS             21611..22690
                     /locus_tag="P5316_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRR84586.1"
                     /translation="MVIAVLLGVLCGVVLLRFLGYFGPPPEPRAITPRGDLMETEKTT
                     IELFEKAAPSVVNITNVALRRDRWRLNVMEIPQGAGSGFIWDGSGHVVTNYHVIHGAN
                     ALRVTLSDQSRYNATVVGAEPDKDLAVLKINAPPGKLAAIPLGTSHDLQVGQAVFAIG
                     NPFGLDHTLTTGVVSALGRTIQSMTQRTIDDVIQTDAAINPGNSGGPLLDSAGRLIGV
                     NTAIYSPSGSSAGIGFAVPVDTVNRVVPQLIAHGQTVRPRMGVRLLSERLNAQLLAGL
                     GVKGVMIADVEEGSGAAAAGLRGVRQTPEGDILPGDVIQKVDDRNIDSIDGLLNVIEK
                     HKAGDTVRVTFLRDGKTQTVDVVLQ"
     gene            22704..23285
                     /locus_tag="P5316_06205"
     CDS             22704..23285
                     /locus_tag="P5316_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRR84587.1"
                     /translation="MSAKKILMIVGDFVEDYEVMVPFQALQMVGHTVHAVCPGKKAGE
                     KVRTAIHDFEGDQTYSEKPGHNFTLNATFAGLSPDDYHALVIPGGRAPEYLRLNEQVI
                     KIVQHFVRTKKPIAAICHGAQLLAAAGALQGKTCSAYPAVGPDIAGAGGTYVEIGIDQ
                     AHVCGNLVTAPAWPAHPAWLAKFLEVLGTKIEP"
     gene            23436..23645
                     /locus_tag="P5316_06210"
     CDS             23436..23645
                     /locus_tag="P5316_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84588.1"
                     /translation="MTYKGKVQGGVVVLEGGAALPEGAEVVVEPVEDLPSWAEVFKDV
                     LGKATGLPKDLARNHDHYLHGAPKR"
     gene            complement(24145..24813)
                     /locus_tag="P5316_06215"
     CDS             complement(24145..24813)
                     /locus_tag="P5316_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84589.1"
                     /translation="MSSTRLTIVVLLLASCGLYGCAPSRRPSPAQVDDFINRRAFVIS
                     AINDIKDPMPGTSYSGISENYASDSEKRIKRELEDSAFLMEGIRQEIDQRGSYVVEPG
                     LGIEFDVDLSEQLTEKTDREAIARQLNHYYVRKLEKAGWYVGACRKGGVSPGAVTVTY
                     IWTDRGKGSHTYSKDYSSMSLQSTFRDPEDYRRGFFHVLIAVRVDTDVNRAHVAINYW
                     GRIG"
     gene            complement(25017..26069)
                     /locus_tag="P5316_06220"
     CDS             complement(25017..26069)
                     /locus_tag="P5316_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR84590.1"
                     /translation="MSQSKPTRREFLKTTSVAMASVTLAGQASAVQTRPADTSKILNY
                     NPKMGYRRLGKTGLMISEISLGGHGGKTVEDRVPVLERAIELGMNYVDNNIVEECALY
                     GKAMKGKRDRWYIGFASWPEKLTSEYEKELSKEGMAKAIDDRLKAYDTDMLDIWRPVG
                     ATWGEGQTKQETMLEISPRVLDMVVEVFEKARTQGKVRFLGVSAHKPEVFRRVLNDYP
                     QFSVILFPYMFLTTAFKGDSLLDLAQKKDVGVIAIKPFAAGATFVGGTPTKLEGKVDT
                     RASSLLKAMLAEKRISAVIPGVTAAEQLDENVKASYERDKPLTDKDKQALRECTESFH
                     AHLPARYQWLHEWKTV"
     gene            complement(26086..>26754)
                     /locus_tag="P5316_06225"
     CDS             complement(26086..>26754)
                     /locus_tag="P5316_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine oxidoreductase"
                     /protein_id="HRR84591.1"
                     /translation="CATCHMSATKTQPLNHDIGLRISWNNRPAISIRPEVSDKKMGLP
                     GADIDWKTRRANMQNVCLNCHEKQWVDNFYVQYDSLVELYHDKFAKPGLELYELAKPL
                     LVPVEFANKIDFVWYEIWHHEGRRARHGASMMGPDYTHWHGTYEVAKHFYSEYVPELR
                     ELAHANANSSDAAKAEAAKKLQARLDEVLSSPNHSWYIDKLDPAETQRRAKAAEEFRK
                     RYGK"
BASE COUNT         5361 a         8552 c         7887 g         4954 t
ORIGIN      
        1 gtcccagatg ggaatctggg accagcgggt cacagccccg ccaagtcgcc ccgaccaagc
       61 acggtgccga cctgccgccg tctcgaccaa cccccgcgcc aaacgaaccc aacccaaaaa
      121 ctgctaagcg cctgtagcag aagtgtttac gcgaataacg gacgtttctc ctcgctgctt
      181 taacccaccc ccgcgatgcc tttccccatc ggctcatgcc cgttattcca gcggaaccct
      241 cgtcagactt ggctcaacga ttcttgccgg gctggggtcg actcccaagg gcagggctca
      301 tctctcgatt cctggcatgt tggccatcgc ggatcagata ccatgcgagg cctggccaag
      361 cccatttcgg actgaatgtc cagcggtggt gtatcatgta actgtccggg acaatgagcg
      421 cagaacagga gttcgcgacg atgagaaccg tcgccggttc gttggtctct tccgtcgcga
      481 ctggtgtgcg tttcaacgaa gcttttccgc cggcgaccag accaacctcg cttgccatgg
      541 ggccatcctc gcggcggaat gactctcttc gttttgcgtg catgtttctg cagaagtgca
      601 gaactcgttc agcaccaaag acttgcacgc gtaattcacc tgtcccgttc aggttgcggc
      661 cgaaggccgc cttggggatt ctgctttgca tgtgcgggtg tacgccgacc gagtgtcaga
      721 gacgccgctt ttcgcccgag ccacaagcct atttctgcgg attcagcgtg ccaaaccagg
      781 gacgctgcca ttcgacgtgt ccgtcgaaca tggtgacgtt gattccgccc gaatggcaag
      841 gccccttggg aataagcgtc aagccattct catgtacaaa gtcgagctga taagtgccct
      901 tcttccactg tccgggcttc agcgtcctgg ccagctcgag cggggcttga gggtcccaac
      961 gaaaagcatc accgaaagcc cactctctgg ccggctgtcg aatgagcgaa atcttcttgg
     1021 gtgccgcatc cttcgtcagc accgtcggcg acgccaaagg cgtgaaaggc ggtttcgtgt
     1081 tattccagta gtggtctggg tacccgaaat accaaggcgg attggtgccg tattccacct
     1141 gcgtgctgtg attccggttc cagttgttca gagcgtaggt gaacggacgc cggaaatcgg
     1201 cttccgcgaa cgccttctgg tagctggccg ccgtgagcgt gtaggccgtc gggcaactgg
     1261 caacctcgtc ggtcaggcga ccctgccgtc cgcgatcatg aaagtatctc cgaatgaagt
     1321 ggggaagatg ccattcctcg taatccttgg aagcaacctt cccggccgtt tcaagctcca
     1381 tggccccatg aatcgggccg ggcagcacat ccctgctgtc aagcagatac atcaaagttc
     1441 ccttggcgaa ctcccgctgt tgagcggcgc acaccgctcg tcgcgattgc tcgcgggcct
     1501 ttgatagcga tggcaagagg atcgccacca gcagcgcgat gatcgccacc accactagga
     1561 cttcaatcag cgtgaaggcg gatcgacccc aactggcttg cctacctcgt gcccccgtcc
     1621 tgcgttgaac aagatcagcc atacctgcct cgctcctgcc tcgtcgggcg cgggatcgcc
     1681 acgaattgaa tctcccgctc ggcgagccgc accgagatgc cggcaacgca tctgtctcgc
     1741 ttcgaactga tgcccatatc gtacccgaga aacgttatca gaacaaccgg gtttactcgt
     1801 cgtgtccgtc ggccctttcc ccagatgccg gccggccatg gggtaaacaa ggcgcccggc
     1861 agatccgtca gaaacccctg gagtactcat gaaccgcatc taccagggct ctggcgtttt
     1921 ccacaggggt gttcggcaac acgccgtggc cgagattgaa gatatgtccc ggccgcccgg
     1981 cggcctgatc caggatgatc ttggcctggc ggcgtatctc gtcagcagag gttaataaca
     2041 cgttcggatt cagattgccc tggacgccga cgtcgtaccc gatcgttttc caggcctcgg
     2101 ccaggttcac ccgccagtcc agaccgatca cgtcgccgcc ggcctcacgg atcgccttga
     2161 gcatggaacc ggtattgacg ccgaagtgaa tcacgggcgt gcccgcactg acggcccgga
     2221 ttagcgactg cgtgtgcggc agcacgaagc ggcgatactc ctgctcgtcg aggcagccga
     2281 cccaactgtc gaaaacctgc acggcctgtg cgccggccgc gatctgaccg ttcacataat
     2341 cggccgacgc ccggacgatc cgctcgagca gtgcattcca cgcacccggg tcgtcgtgca
     2401 tcagcgaccg cgtccgggta aactcgcgac tggccccgcc ctcgatcatg tagctggcca
     2461 cggtgaacgg tgcgccgcag aagccgatga gcggaatgcc gggcgccagc tcgcggcggg
     2521 caatccgaat ggcctcgaaa acaaaggaaa gcgaatcgtc gacatcgacc tctctaagct
     2581 gattcacgtc gacgccgctt cgcaccggcc tggcgatcga gggcccttcg cccttgttga
     2641 acgacagccc cacgcccatc ggctcgacga tcagcaggat gtcggcaaag atgatcgccg
     2701 catcaacacc gagctgctcg acggccatca cggtaacctg ggcggccagt cccggcgtct
     2761 tgcacaactc cagaaacgag accttctcgc gaatctcgcg gtactgacgc tggtatcgcc
     2821 ctgcctgacg catcagccag atcggagtgt aatccgcctt ctcgcggcga caggcacgga
     2881 gaaacgggct gttgtgaagg acttcgcaat ccatggcgtc tgcttcgtct ctgcctgacc
     2941 agacatatct ggaacgaggt gcggcggata aactgtcggg cgatactcag gctggacaac
     3001 ccaatcaccg gcggccaggc tgccggcggc aaccgtcggc cggatcaaag cgagaccgtt
     3061 ctgccgctcc gggacgactc gtagatcgcc aggatgatct ccaccgcttt gcgagcctcg
     3121 cggccggaaa tcggcggctc cttgcccgca tcgagggcag cgaggaactc ggcgaagttg
     3181 gcgcggtgat tgtccgcggt catccctgca gacggattgg ccgccccgac cgaaacgcct
     3241 gggaactgaa gatacttctc gcggatccgc gcgtcctcgg gcgtctcctg ggcgaattgc
     3301 cagaagaaaa acttgttgtc ggccatggcc gcggttccgc gatcgccggc tacctcgatg
     3361 attcggaagt gccccggcca catgctggtc gcgcaggcga tggtaccaat cgccccgttg
     3421 gcgaactcga tcgccgccac gccggtatcc tcaacctcga tccgctcgtg ggccagcgtc
     3481 cggcaaaaag cggtcaccgc cttgaccggc cccatcagcc attgaagcgt gtcgatggtg
     3541 tgaatgccct gattcatcaa ggccccgccg ccgtcaagtg cccaagtacc gcgccacccg
     3601 ccgttgtcgt agtaagattg ctcgcgccac cagggaccat aggccatgcc gaaggtcatc
     3661 cgtccgaagc gaccggcatc gacggccttc ttgaacaact tggccgtctc ctcatgacgc
     3721 atgttgaaga tgcccccgac ggtggtgccg gctttgtcgt gggcctccag gatccggtcg
     3781 atcttttcca gtgtgatctc gatgggcttt tcgaccacgc agtgcttgcc gtgcctggca
     3841 gccgcgatgg ccacctcacc gtgcagcccg ctcggcgtcg ccaccgtgat cacgtcgatg
     3901 tcatcgcggg acagcagctt ctccaagctg gtctcggccc cacacccaaa cttgccggcc
     3961 aaggtctcgg ccgccgacgc aacctgatca caaatagcca gcagtctcgc gttcggcaga
     4021 cccttaatcg cctcggcgtg aaacttgccg atcatgccgg ccccaaccac accgaatccg
     4081 tatgtcttgc ccattaccat caaacctcgt ctttgctcgc ccaaaaacgg caggcaaggc
     4141 ccacgccact gaaaaccgac gactgagaac tgacaactac ttcttcaccc tcagccacct
     4201 caaccgcagc cgatccctca ggaactccgt gagtcgcaac ggaaaccggg acacgttcgt
     4261 ttcgaaggac agccgccgcg atcgttcact gatctcctcg gaccccggac gccatacgtg
     4321 gagccggatc gccaggtctg ccacgaacga ataccacttt cgccccgccg cgttcattgt
     4381 cgctgctctg taggggtggt ccccaaacat gatctcccac gtagcccatc gccctggagg
     4441 ctgcgttctt gtcaacaatt cgcagccccg ccgctagagc gacgggccgt cagggctgac
     4501 ggctaagagc taatagcttc ttcaagccat cttccgttgc acggccaggt gcgtcgtcgc
     4561 cagcacttcg tcggctgagc gcgattggct cggcatcggc cacaactgct gccgcttgat
     4621 gaccgttgct ccggccgcgt aatagtcgct cacctggctc gggccgccct cgaccaggtc
     4681 gccgccttcg taaccgatcc gattcagcca atcggacacg tcgtgcgtgt actcggccgg
     4741 aaaaatcgcg accgtgcaca gctcgtatct ggaaccggaa atcctggcta aatgaacctt
     4801 catccggcac ggcggcggag tgccggccgc ctcacgaacc agggtctgga agtcctcctc
     4861 ggccaggccc ggctcgtcac gatagaagaa caaaccgtct tccttcagaa acgtctcccg
     4921 cgacacgccc tgcagttcca tcgtgccgtc cggcatggcc cggtgaatgc ggtatacctt
     4981 gccgtcggca taccgctgcg actccagtag gacggcgatc tcatcggccg tgtatccgac
     5041 ggccacctga tcgccaaaat caaacacgta cagcccccgg tacttctccg gcgattcaag
     5101 ttcgggcagt ctcatggtcg tatctccccg gtcagcgtgc cccaagacca aggtacgcgg
     5161 aacgagagcg gtggtcaagc aacaggtcca aggcggatcg cctttttgcg ggccgtaacc
     5221 caagcgcacc gcgatccgga aacatgcgcg caagccgcac agatctctgg ccgtaaggtt
     5281 ccaaggcgga cgcaataccg atcgcatgtt ctgatgcctc attcggtctt caagaagagt
     5341 ccgggaatac tcgaccgcgg ccgccgggtt gcagaaggcc gctctgccgc cgcgcgtcgc
     5401 caagcaggca agggtcgaat acgctcatcc gatccgcact tcgcagcggt tgcgatcgag
     5461 atgatcgaca gccacctcga tatcgaggaa cttttgaatg aggtccatgt gtgtcgtcga
     5521 atggagggtc aacggcgtgg ttcggtagcg gccacggccg gcgatggcca acgggagcat
     5581 gatctggtcg gccaggtatt cgcctaccgg gacatctgcc ttcagccaac gctgatatgc
     5641 ctgcagcgca tgcgtggcga cggcttcggc tgcccgtccc agtgctccaa agccggtaaa
     5701 cacctcggcc acgttctcgg cctcaacctc gatgatgacg acgttgccgg ggccacgggg
     5761 gtgtgcaacg ttctcgacgc tatagcacga ctcgccccaa ttcgtcccct tacgtaagac
     5821 gttgatctcg cgctcagcta tgtggccggg caggttggac acgacggcac ggccacaccg
     5881 gcggatgatc tcgccgcgtt ccgtaagctc aaagcccttc agctccctgg caggttcgat
     5941 ggagatcgtg aacttcccgc cgccggccgg gtagaagccg ggacgctcca gctccgccga
     6001 taccgtcggt cccatccggt tgaccagcgg gatgaacgcc ttggccagga aatcgaacgg
     6061 cggggcaaac gggttgtgcg tgccaccctc gaaagtcagc cgcgacgggc cggaggccgt
     6121 gagcagggcg ggcaggaccg tttgcagaac cagcgtcgta ctgccggccg taccgatgct
     6181 gaaggagtat tcgcccggtt gaacctgctt gggatgaaac tcgacgacct tcgagccgat
     6241 atccgcccca cgcacgtcgg cttgcgaaat ctgtgccgcc gcctgcaccg cggtcaggtg
     6301 ctggcgcatc agccccggct tcttgcgccc cgcccggatc ttttcgatcc gaaacgcccg
     6361 ccccgttacc atagccaacg ccagcgacga ccgcagtatc tgcccgccgc cttcaccgaa
     6421 tgaaccgtca atggtcaaca ttcaatcatc cgccttgtcc gcacacctgc tcatcaaaca
     6481 aacgttgagc tcttccgggt actcactaac aacacaatgt cttctggcgc gttgcgggtt
     6541 gaggattcct atcatcatta tcgtcgaatg cggaacggat tctgccaaga cccgcggccc
     6601 ggaacgaatt ccgggccgag cggatgatga agaccgtggg gacgagcccc acccctcgtc
     6661 aagggcaatc aggccttctc gctgaagtcc tgccactcga tcccgacctt gccgagaacg
     6721 tagccgacct cgcgcaggta gtcgccgtag gtgacgacgg cgtggaagcc ctgcatctcc
     6781 cgggccatac gccgccagtc gccgtcgatg gctacgtcca tctgcgagcg gcacatgtcg
     6841 tagtgcgggc tgtcgacgat cctgccgcgg aagccgaacc acttggtgca gttcagattc
     6901 ggaatgatga cggtgacgac ctggccctta tcgtattcca ccttggtggc ggtgccgtag
     6961 tccgactcaa aatgggtcat gatcttcgtc ggggcgtagt cccggccgtt cattcgccgc
     7021 ggggcggcgc agtgggccag ggtaatgacg ccgtcgtgcg gaaaatgcgt attacagacg
     7081 aacgacggct tgcccgagat ccagcgcatc agcacccccg gagccgtgtg cgtgtaatcg
     7141 gtgtggcaga aggcggtgaa tccctcatca ttcagcatac ttagcaccag acacggcgcg
     7201 gtgtcgagca ggccgatcag cgatcccatg cagtgagcaa caccgaggtt ggcggcgccg
     7261 ttggccttca tgatctcctt gaagatgcgc aaggcaagga atgagttctc gacgaatcgc
     7321 ttttcgatac tcgaatccac attgggctgt gccaggaact cgtccgcttg tcgtcggatt
     7381 tccttcatcg ccgactcgtc cttgcgggct ttggcaagct gctcggcaac gtcctcgtcc
     7441 ggcacggtga tcagctcgta gccccagacc tgcttgacat ggtccggtcc tttctgttgg
     7501 cccgggacgc tgtacgcccg caggcctcca atggccacgc acctggtgcc tttggcgttc
     7561 tttaggccgt gcaaggcccg caaccgccac aggatctcgc ccgcgtcatc aaccacgatg
     7621 tcatcgaccc cgattcgcgt ctctttgatc agatcctcat tcttgcggag ccatcgccaa
     7681 tgaacgatct cgtaatacag gtaaacgggg ccggatttgt gccgcacgaa catgacgacc
     7741 ggtttgcctg aggcggcaat cgtctccagc catgcctggt cgccgcctgc ggcgaagaga
     7801 acgatcgcgt cggcatcact gctcgcaacg ctcttggctt ggtcagggcc ggcaaccatc
     7861 gccggcggct gcatctccag cctgaactcc gcttgtgacg acaactttgc gagatcctgc
     7921 tcgacgcggg caacctccgc ggccagatcc tcttcggtga ggatgccgcc gtacggccgc
     7981 caactggtct tctcgcgccg ctgcgggatg ctgtacatca acgcaggctt gaccttgagc
     8041 ggagccccca tcggcaagcc ggagccggcc gattggcctg cctcggccgc ctgggctcgc
     8101 gaggcagcaa gcatcgccag cccgcccacc gccgccgccc cgccgatgcc gcccagcaga
     8161 ctccgacgac tgatcccggc cgcgcgctca tccggatgac cgcagcaagc gccgccctta
     8221 ctgcctgaat cacgattgat gtcgtgtgaa tcgaattgca ttgcgggtgc ctcctaattg
     8281 atcgcgaacc gtcacttcga aatgccacag cgttgcgtca tgccgcgccg gggtccatct
     8341 taccccagat cgattgttga cacaccagcc ggcatttcgg atcggccgac tgacacggtt
     8401 ggacgcctgg aggcctttcc gatacgctaa gaatgtgcgt gaaaacggta gggcatggtc
     8461 ggagccaccg aaggtggcgg agacctgccg gtcttggctc ggtctgacaa gctcgaagcc
     8521 cagacatgcc atagaacatg accttgcagg catcctcata gaaaggaaca cccatgaagc
     8581 gtcgtgattt cctccgcaag tctgcggccg ctgcggcggc cggcgtctcg tttccgtatt
     8641 tcgtgcccag gcacgttctg gcggcacccg gcaggccggg tgcaaacgac cgattcaagg
     8701 tcggcttcat cggctgcggc ggccgggccc acgctctcat gaatacggaa cggctctccg
     8761 accatgccga catcgtggcc gtcgccgact gtttcctgac ccgcatcgac gagcgggcca
     8821 aggccacccc cgacgctgat aagtgggtca gataccagga ctaccgcaag atgctcgaga
     8881 aggaaaagct cgatgcggtt tttgtcccca ccaccacaca tgcgcgggtg ctggcctgca
     8941 tcaccgcaat ggaggccggg tgcgacgtct acgcggagaa accttttgcc ctgacgatcg
     9001 ccgagggccg ctcgctggtc aaggcggtca agaagtacaa aaaagtactc caggtcggca
     9061 cgcaacagcg aaccattccg atcaacgcct gggccagcaa actgattcgc gacggcggaa
     9121 tcggcaagct caaggaggtg caagccccca acttcattgg gccggatcgc tggaaaccac
     9181 tacccgccga ggagatcccg caaggccttg actgggacct gtggtgcaac cagaccgagt
     9241 tgcggcccta ccacaaggag ctgcattacg gctgggccaa gtggtgggac tacgacggcg
     9301 gcggcaagtc ctggggcgtc accggctggg gcacccacgc cttcgaccaa gcccaatgcg
     9361 gaatgggcgc cgacgacacc ggtccgatcg aaatctggcc ggagggcgcc gacgcggcgg
     9421 gcaacgtcaa ggtcacgatg aaatacgcca atggcacact cttgaaatgc cacaatccgc
     9481 tgcggttgaa ggacgataag ggcaacaacg tgtaccacga cctcggggcg atcttcgtcg
     9541 gcgagaaggc cacactggag atccgccgcg gaagcctggt ctgcgatcat cccgaactga
     9601 tcaaggatgc cccgccgggt tacccggcgg ccactaacgg cgaggtggtc tggcatatca
     9661 aggacttctt cgactgctgc cgaagccgca agcagccggc cgcccacgtc gaggccgctc
     9721 agcgggccac cactctgtgc tatctggtga acatctgccg ggacatgaac cgcaagctca
     9781 agtgggatcc gaacgccgag aggttcgtcg gcgacgatga ggccaacagg gaactctcac
     9841 gcccgaggcg caagggatat gaactgccga aggaattcac atgattaata ttgttttcat
     9901 gatctattcg tagtaaaggc ttcattgtgc tgaatgctgt gcgatgaagc gagcgactgc
     9961 ggcggggtgc gtcaaattga tgaaatgccc tccgtcggca atcgactgct cggcctgcac
    10021 accgccaagc gggatcatgt ggtcgcgtcg gccgtgaatc tgatgcaccg ggcagtcgag
    10081 ccgggagggg ttcctccaac ccaagatcat ggcagcctgc cgtcggagtt ggacaatcgg
    10141 cacgtcccgg cacatgtcca gaagcagccg atggtgttcg tggccaatcc cttccattcg
    10201 ggcaaccact cggcttaccg gtccggagat gccacggatg accgtatcgg gtaccagctt
    10261 ggcggggtga aagatcagcc aattccgctt tggaatagca gccgaactgc ggcaactggc
    10321 aatgaggatg acacgcttgc agttgcaccg ggcagccacc tcgcaagcca gcatgccccc
    10381 gaaagaaacc ccgcccagaa cgcagggagt ccgcccaagc cccaaatcgg acaccacccg
    10441 ggccgcgtaa gacggcagat cctcgccatc tcgcgggacg ggcagccttg gacactcgaa
    10501 gcggacacca aagctgctct ggggggagaa aagacgttcg tcacaagcca tacccggaaa
    10561 cagaatcagc ctttgcaacg ccatcacaac tattggagcg gcatacccct tccctggtaa
    10621 cccgcttgct cttgataggc caacaagcac cagcggcagg caaatcgcca gaccaagtcc
    10681 taagatttgg tcctataagc gcttgcggga tgccggcaaa atcgccaatg ccaacactgg
    10741 caacaacttc cggttgctca gacactgtgg tctttgtagg atgaccagta taggaggcaa
    10801 ggtcggccat ggcatggtct gaccggcgag gggtggtttc gcgaaatagt ccacattttg
    10861 cctgaggaca cctgagatgt tcatgcaacg cttgctcagc cgcacggtcc tggctgtgac
    10921 ggtaatcagt ctgttgtgcc cgatgctgat ggggccgggc tgtcccaatc tcgagatgat
    10981 accgctgaac aatgatcccg aagccttgca gccgccggac gactcttcgg gcagcagcac
    11041 accggctacc ttgcagatct acgccggtcc agatcgtgag gccacggtcg gttcccccat
    11101 caccctgtct gccacggtcg aaggcggaga agaaccttat ctcgcctcct ggtcgccggc
    11161 ctcggcattg agcgacgcca acaccctctc accaacattc gtccccccgg ccgagggcga
    11221 atacgaattc actctgacgg tcgcggatcg caaagggctt ggcggcagcc gcaccgtgaa
    11281 ggtcaacgcc ctggatcatg ccactctggc aagcctcaaa tgggccccca acttcagcga
    11341 tggcggttac cagttgatcg ctgcgttcac caagccgttg gacaaaccca ccgctgagaa
    11401 cgtcaacaac taccgcacga cccccaaggc ggatgatacg cgcgcggcct cgccccgtga
    11461 gattgtcgcc aaggtgagtg tgaagccgac cagtgcgacc ctgtcttccg atggcttggt
    11521 ggtgacgttg ctgttcgaca gcaccacgct ggccagaaac acgcgtttcg acatcagtgt
    11581 cagcaacggc attctcgggg ctgacggggt ggcggtatcg gaaatcaagg gcctgccggc
    11641 tgccgccgac agtgccgaca cgaaagcccc gaccgtcgtc agtcgccgat gggtcaccgg
    11701 cacgagcgta agcgacgcgg agaccgccgc ggaagacaag atctgggaaa acagcggcgt
    11761 ccataccgag gtctcttaca gctacatcct gcaagtggtt ttcagcgaga cgatggatcc
    11821 cgtgccggcc gtgaatccgg ccgcttatcg catccaggaa gggaccaacc gatacgctcc
    11881 ggcgatcgtg gaactcgcca acgacggtcg aactgccacg ctcatcttca ccgatgggcc
    11941 gttgtcccag gaaagcaagc tcgacgtggg gttgacccgt ttgcgcgaca tgaacgggct
    12001 cgacttggtc ctcgatgcga gcaagtctgt tgctgccaac gccgacgaca aggtcgcgcc
    12061 caagatcgtc gcggaatcgg tgcgattcct gagcagcagc ggcgagggct atcgtgtgga
    12121 gatcacgttc gacgagccca tggatcggaa gacatgcgag accgccgcga actacctgct
    12181 ggacggccac gcggcctcga cggcggaatt gggcagtgac ggccgccgcg tggtgctgac
    12241 atttgaaggt ctcgccgcga ccactaacag caaactgagc attccggcaa acaaagtgaa
    12301 ggacattaac ggcgtctccc tcgcggctca gaacaatctc agcatcctct ccgccgacga
    12361 ccccatgacc ccaaggggcg tcccgacgct gacctggctg cccggcgata agaccacggg
    12421 ttatgaaatc tgggccgagt tccccctgat catggaccgg gcaacggtgg aagacgtcag
    12481 gaactggcgc atcacgggta ccgacctcaa tccgaagtcg gtggttttgc actcgaccac
    12541 aaacgcggcc gccggcacca ttgccgggca aaccgccaag atcaccttct gggatcccgg
    12601 cctgaccgac cagacggagc gaacaagacg cgcgcggctc ttgaatcgac gcacgcaatt
    12661 ggacgtctca gtgggagcat ccatacggga catcagcgga acggcgatcg ggcaggtgag
    12721 catccccgtc gtcgccaacg agaatgacgt cacgggtccg cgcttggtac ctctgccggg
    12781 gcagaccgaa gccaagccga tctggggcga cgtcctgaag agcgagtacg gcaagactca
    12841 ctacagcgtg tctctggctt tcagcgaaac catggacagc ctctccgtgc tgaattccga
    12901 gaactatgtg ctcggcggcg ttcgacccac ggcgattcaa ttggatctcg acggccggac
    12961 cgtcgtgctg accttcgcaa aactcacgac gccgctgggc aagtcggacc aactgcgact
    13021 gtacagcacc ctgagggaca tcaacgggcg cgccaacacc agcgtgaccc cgatgaccat
    13081 cttgcccgcc agtgaaacga cgcccccgac gatcgagtcg ctatactggc tggccgacag
    13141 tcggccctac cgggtcgtgg tcaagttcag cgaagtcatg gaccgggatt cggtggaagt
    13201 tgtcgacaaa tggcttctcc tcgactacgg cggcatcgat cggaaaccca cgcaggccgt
    13261 gctgaatccc tcagagccca caacgctcgt tctcaccttc gaaaacgggg ttttcgcgac
    13321 cgactcggta ttgtcgctgg acggcgaaat ccatgacatc aatgggaacg cttatgacgg
    13381 gtcaggtggc ggaatcgtcc tgccggctgc gcccgccgac gacgcaaacg acgtttcggc
    13441 cgcgcgactt ctttcggcca cgtggatggt agattccaac ttgggatacc tggtgctggt
    13501 ggagttcagt gaagccatcg acgccgatca tgcaggaaca ttcaccctgg acggaatcgg
    13561 cccggcgtgg accatgatca ccgatggcgg gaccctggtt gtcgcgttgt tccccgaccc
    13621 ggcaacgccg gcggttttca accgtgactc gcgaatcgcc atttcgggct tgcaggacat
    13681 gagcggcaac gtgtccacaa ccatggtgcg gaacgtcgcc gccaacccgc tggatactct
    13741 cgggccgaca ccgctgcggg ccatgtgggc ggcagattac ggcgacgccc ccgcggagca
    13801 aggttatcag gttattgtcg tattctcgga agtccttgac gctcttacag cgcaggtcgg
    13861 tgccaactac cagatcaccg gaacattctc gaagccgacg gaggcagcac ttagtccaac
    13921 cgacgggcgg acggtcgtct tgaccttccc accgaccgtt ttgtaccgga gcctggcagc
    13981 gaccgatacc ctggacgcca gcaccggtaa ctcgatcctc gatgtgaacg gggtattcgg
    14041 ctggcaagcc actgtggaca ttacgcgcaa cagcgccgac acaacctccc cgacggtggt
    14101 ggcggcgacg gccgtgggcg gaaccggggt cgagatcctg ttcagcgagg ctttggaccg
    14161 cgtctcggcc gagatgctgg acaactacgt gctgacgggc aatgtgtatc cgacgacggc
    14221 gttgctcaaa accgatggcc ggaccgtgac cctgagcttc gaccagtcgg tgagcgggaa
    14281 gaatctccgc gtcagtgccc aggatacgat cgcggacgtc aacggcaatc gtctcagcgc
    14341 aaccgacgtg ggtccgctgt aacagccggg cttgacccgg gccgatcgtg ggagcgaggc
    14401 accgataact ccgcccgaac gttcgccggg cgggtccgtg atcggttatc gggggcaaag
    14461 gatgcttgtt tgtcgagaca accggctcct cgtagaatgc cggaggatat aatcagcagt
    14521 ggtgttgagc ggcacgcgct gggccgtcgt cggaggggtt gttggcattc cgtattgcat
    14581 cgaaggatat gatttccatg gcaagaaaac atgtggggaa gcgagtcgtt ctggttttct
    14641 gggctctttg cctgggcacg cccctgctta tgggccaggg atgttgtgaa ggtctgtggt
    14701 tgctcggatt gcttggcgcg ggccagatgg cggaacccag gtgcccgacg ctgatcaacc
    14761 cgctgaccgt cgatgcggga gacgacttgg ccgtcgcggc aggcggtaac gtacagttcg
    14821 gggcctcggt cacgggggga accgtcccct acatctttgc ctggtcgccg actaccggtc
    14881 tgagcgatcc cagcgcgctc aggccaacct tcaccgcgac aacggcgggg cagttcccgt
    14941 ttaccctgac cgtcaccgac gcggctggct gctcgatcag cgatgccttg attgtgaccg
    15001 tcagcgccgg cgatggagat tccggccacg gcatcggcgg tgacggcggt gatggcggcg
    15061 actcgggcac cgatggctct tcgcaaccgc agcccctcgt ggtagacgcc ggcccggatc
    15121 gtacgggaac ggtcggcgcg tcaatgctgc tccaagggtc tttcaccggg ggcaccgggc
    15181 ccttcaccat ttcctggtca cccgctggtt ccctgatcgg accgaatacg ttgaccccga
    15241 cattcacgcc gacgtcggcc gggaatttca cgttcacatt gaccgttact gacggcaccg
    15301 gggccagcga aagcgactcc atggtcgtat cggtctccgc gatgacctcg ttgagcagcc
    15361 tgaggtgggg ggcgaacttc gcgggcgaga gctatcagct tctggccgag ttctcgaaag
    15421 cggtcgataa ggcatccgca gagcgactta cgaactattg cgtcagcggg acggaaaccc
    15481 acccggtcag cgccgtgttg tcttcggaca tgcgcaccgt cgttctggtt ttcgcggacg
    15541 tgaaactcac agccaaggcc gagttcgata tcagtgtcgg aagcggtgtc ctcgacagca
    15601 acggcacccg cattccgcag gtcaaaggcc tcgtgcccgc gccgaacacg gaagacacaa
    15661 ccgcgccgaa ggtcgcattg atccgctggg ccgtgaacta caacggcagt tacggggtcg
    15721 agttgatctt caacgaggcg atggatcagt cgacgctcga aaacgcccgg agctatgaga
    15781 tccatgacaa cggtgtcgcc gccgtcgcct tcgacgctca gatgggcctg gacggcaaga
    15841 cggttaactt ggtgttctat ggaataccat tgtcgcaggc cgccaaactc agcgtcggct
    15901 tattgaccat ccgcgacatc aacggacaga gattgcagcc tgtcgatcaa caggcgatct
    15961 cggcgaacag caacgacctt gacccaccgg tcgtggtcga caactcgatc cagttcgtgg
    16021 tcgattattc ggcaggcgga tatcaggtgg tgctcgatct gaacgaggca atggaccgcg
    16081 tctcggtcga ggatccgacg gcgtacctta tcgacggcct ggccgcctcg gcggcgaagt
    16141 tgggcgacaa tggccgccac gtcactctca aatgcgcgaa caactcctat gcgccgacca
    16201 gcaagctctc gatcctgggc aacagggtca aggacatcaa cggccggtat cttgccgccc
    16261 ggtccaactt ggcgatcctg cccgcttcca gcaccggcgc gattccaagc gtccctgtcc
    16321 tgacatggct caagggcgac gagtcgaccg gttatcagtt cctggcgcgg ttcaacgaat
    16381 caatgaataa gacgacggtg gaaaacaaga acaactggcg gatcagcggc acgaacattc
    16441 gtccgacatc ggttgtcctc agcacgcaaa ccgtcggcga cgaaatcgcc gggcgcaccg
    16501 ccaaggtgac attcggcgat tttggcgaca gccgcaccac tatgagccgc acgaccaggg
    16561 ttgatgtctc cgtcggcgac tcgatcaccg acatcaccgg gcagagactc gcccaggtca
    16621 cctgcgcgat cggggctcat cccggggaca ccgtctctcc cttcgtggcg gccccctaca
    16681 gcggcctgcc ggccagaccg gtgtggggtg acatcctttc gcccgacgga tatcgcggag
    16741 atacctacgt cgtgtcggtc gtattctctg aaaccatgga cgcaacctcg acaatggacg
    16801 cgaccaacta ttatctggtc ggcgtccatc cgacgtcggt cgtactcggc ccgaggggtc
    16861 gcgaggtgat cctgacgttc gcgaacatcc ccggcggcct gacgtcggct gaaaaactga
    16921 agatcctggg caacgtccga gacatcaacg ggcggcgtac ctcaagcgtg gtgtcgcttc
    16981 cgatccagcc ctcgcccgat gacatgatgc ctccgggcgt cagcgacatg ttctggggaa
    17041 tcaacaaggg cccctatgaa gtggccgtca cctttgacga aatcatggat gccgagtccg
    17101 caaccaatgt ggccgcttac acgattgatg ggttgaatcc gacggcggcg atcatgcagc
    17161 ccgacggaaa gaccatcgtt ctggtcttcg gcaactcgtt actgactccg ttgcttgagt
    17221 tgagcatcgt cggcgaggtg aaagatctca acggcaacgc ctacgacggc tcccacggtg
    17281 atgtgtcctt gccggcggcg ccgagcgcac gacccgaaac tgaccggcaa gcggcgcgac
    17341 tggcatcggc cgtttgggcg gtcgactcgc tcgactatcg catcttggca acgttcaccg
    17401 aggccctcaa cgccggcacc gcaggaaacc ccgactatta cgaggctgct gcgggtttca
    17461 atcggttcaa agcgaccagc gccgtgctga tgccgggtgg aataaccgtg gaaacggtgt
    17521 tcgccgattt tggcaacccg gtgccggggc agttgcctcc cagccccttg aacaggacgg
    17581 cacaactcaa cgtcgtccag tccatgggcg gtgtgacgga catgcacgga tggttgtcgc
    17641 gtacctcgat gatcgtcggg gcaaacccgc aggatgtcgc cgccatcgag ttgccgaacc
    17701 ccgtcgcaac gtgggccgcc aacttcgacc agggcggtta tcgtctgacc gtcacctttg
    17761 ccggagaggt tctcgatgct cgctcggctg aagagacccg caattacatg atttcgagga
    17821 cctttgtcaa cccgacaagc gcgagactct caggcgtcga tgatttgccg cgagggatat
    17881 acgcgggccg aaccatgttc ctgaccttca ccaagaagcc gatgagcatc gatacgacga
    17941 tcgaagtcag tgtgggcggc tcgatcaccg atatgagcgg caacagtatg ccgcagataa
    18001 ctcaactggc gatcaacccg aaccctgagg ataccacgcc gcccgaggcc ttgcacgcag
    18061 gcccgggcgc gcaacttggg cagatcaagg tcacattcag cgaagcgatg gacgagacca
    18121 gcacggaatc gcctctcaac tacatgttgc tcgttgatcc caacgatccg caggctccct
    18181 tctaccttcc ctatgcggtt tcgctcgaca atgacggcat gacggcttac gtgcagtttg
    18241 atctgatacc tcagaccgga atgttcctgc ggatcgcggg tgtccaggac gtcaatggac
    18301 tgacgatcgt ggaagtggat ctgcggctct gaggcacctt gatcgacaaa accaaaggcc
    18361 ccgtgacgac cggtcacggg gcctttctcg ttgatcaggg ttttctaagt ggcgtggctc
    18421 tctggaactc gcggatccga tccagcgcca caatgccggt cgacgcgccg ggggcctgga
    18481 tgcagtgagc agcggtggcg tttcccagca gggcggcctc cggcatactc aggctctcgt
    18541 gcaaaccgca gatgaaaccg gcacaccaac tgtcgcccgc gcccgtggtg tcaacaaccc
    18601 tatccacttt gtaggccggc acgaggtctt cggcaccgga agcgtcgcgg cagaatgccc
    18661 ctttctcatc catcttcaca acgacgttcc tgcagccgcg atcctggaaa gccttggcga
    18721 tgtcggatgg gtcgctcaga ccggtaatgg cctttgcttc cggctgggag ggaatgaagt
    18781 agtcgagtac cgggagggac ggatagatgt tccggtgcca ctgctcgcgg ggggcgctgt
    18841 cgagatagac ggtgtccaat agtgtcttgg ccccgaatcc ctgtgcgtcc ctgagcagac
    18901 gcgccgtctg ctcgccgtcg aacgtcgcca tcaccatcgt gccggtgaca aagcagaact
    18961 tcgctccgcg aataatctcg agatcgatgt cctcatacgc cagcgtgccg ttcgccccca
    19021 tggtgtggaa gaaactccgt tgcccacctg tgaagacgga ggcgaaagtg aacggtgtgt
    19081 ggataccgct ggtatggatg acacccttcg ttcccacgcc gtacttcttc aactcggcca
    19141 cgacgaactc gcccagagcg tcgttgccga ccttcacgat cgcgtcgcag gcgacaccca
    19201 ttttggccaa ggcgatcgag cagttgacgg cgtttccacc cgtggtcagg gtcagcctgt
    19261 caaacaagcg aaggccgccc ggagggggat aatcatcaat cgtctggccg atcgcatcca
    19321 cgcagcagat tccaacatta acgatgtcac tcatcgcagt ggcacctcat acaaggtcgc
    19381 atggacccgc tccgtctcgg gcagcagaaa ctcttcgatc ggctcgagac ccgccggttc
    19441 cagcttctca aacaatgccg catcaatctc cggatcaaat ccgtacgtat tccgcagacg
    19501 ctccatttcc gtccagtccg ccaggatgta ccggatgccc cgatttctga gccagtggat
    19561 gatctcctga ggatcacgcc gatcgcgaac ggcatcagcc aacgggtggc ggttgaatac
    19621 cgtcgcataa tcgcacggag acaccacgta gtacgaacgg gcctcgccga ccagcatgac
    19681 caccgttccg ggcggtctgg cattcaccgg cttcagcgtc cgggtgccgg gccactgccc
    19741 gctcatgaac caattcgtgt gaccataggc atcaagcccg acgtccccgg tcatcgtccg
    19801 gacgcgggtg tgatcatagt aaacccccgc gagacggtaa aggttccagg cagtaccgac
    19861 gatcagaaga cccaggagcc atcctcggcc accggccctg cctccaaatg cccggccggc
    19921 aagagtcgcc agcggcagga gcatgaccac ggcaaagcga gcgtactggt gggtcgcgaa
    19981 cgtccagaac acggcctgca acaccagaat gaccaacaaa gcaaccgtcc aacgatcgcg
    20041 agccaccacc gcccccacga ccgcaagaac gaccaccatg ccgcccatcc ggtaatcccc
    20101 gatcgtccga ccgacaagcc gcacaaacag aggcttgtcg gcttcggcaa tgccctgcca
    20161 accatgggcc ttctgccaac gagcctcaag ctccgtgtcc cataaccccg gcttggcccc
    20221 aaagaccgaa taagccatgg gaaacaccgg attaccggta ttcatcaggt tccgcaacat
    20281 ccaaggcgaa aacgctatga ggaaaccggc cgcaaaggtc gccatgccaa gcaaacgttt
    20341 ctgcggtgac cggcgacaaa aaacgatcag catggcagca ggaacagcga tcatcggtat
    20401 ggcagtgtac ttgaaaccgg cggacaaacc tgccagaagg cccgctgcca ggcctcggcg
    20461 gcttgcccct tcggctcgaa cgtcggcacg caagaccacg gcaaaggcgc acatgcccat
    20521 ggccagcatg ccgacttcca cataggcgat tcccgccaga taggccgtcc acggagcggt
    20581 cagagctgca attccggtca caagccccga gcggtcggaa aaggcctttc caaccagcca
    20641 cgctgcagcc gcaaagagac ctgcaaagaa gacattgacc atctgagcca tgaatgcggc
    20701 ctcgacggca tcgccccgaa gaaccatcat gagaagcgac agcatctcgc tgttcagcgg
    20761 gaagttgctg tatgtgttgt tcgcgagaaa cccgatacgg ccctgctcaa agaatgcctt
    20821 gggaacggcc agatggtatt caagcacgtc gtagccgtaa gcctcctctg tgccgggcct
    20881 tccccaaagc acgccgggcg gcaggcaggc ggccagaatc gcaatcgcca ggaagggaca
    20941 cgccatgagc caaagccaat tgagccggcc ggattcctgc ccgcgcggct gcggccctgc
    21001 ctgctgcagc gttcgcacgt ccagagccag ccgcgcgatg ccgatcgcag ccagtatgcc
    21061 tgccagggcc aacgccgccg gccggttcaa ccatccggcc gatcccagcc caagcaccat
    21121 cagcgacaag ccgcctgtgc ccaacccggc cccgatgatc agtctttcat gccaaggagc
    21181 ccggcccaga ccgaccagat ggaccagcca gccgccggct agcgaggctg ctgcaagaac
    21241 gaggaccgtc ccgccgccgt caagcgctgc gatggcgagg gcggccatcg attcggtggc
    21301 aataatagcc gccacaagcg ctaccccgac gatcaggatc aaaccggtcg aggaggcgca
    21361 caacgccgag gacggctccc acgagccttc tggtcttgtc tcgagctcct ttgacacgcc
    21421 gataaggtag cttggccggg ccgcatcgca aggccggagg cttcgttggg tcggccgcca
    21481 actgtgggta caatctccca ccggcagccc caatagcagg gtttccggct accgaggaaa
    21541 atcacccatg tatgaaacca tcccgccgct gcagacctac tcctcccgcc cacaaaggtc
    21601 cctgggacgc atggtcatcg ccgtgttgct cggagtcctt tgcggggtcg tgttattgcg
    21661 atttcttggc tacttcggtc ccccacctga gcctcgggcg atcaccccgc gcggtgatct
    21721 gatggagacc gagaaaacga cgatcgagct gttcgagaag gccgcaccct cggtcgtcaa
    21781 catcaccaat gtggccctcc ggcgcgaccg ctggcggctg aacgtaatgg agattcccca
    21841 aggggccggt tccggattca tctgggacgg cagcggtcat gtcgttacca actaccacgt
    21901 catccacggc gccaacgccc tcagggtcac gctcagcgac cagtcccgct acaacgcgac
    21961 cgtggtcggc gccgagccgg acaaggacct cgcagtcttg aagatcaacg ccccgcccgg
    22021 caagcttgcg gccatccccc tcggaacgtc tcacgatctt caggtcggcc aggccgtgtt
    22081 cgccatcggc aacccctttg gcctcgatca taccctcacg accggcgtcg tcagtgctct
    22141 tggccggaca atccagtcga tgacccagcg gacgatcgac gacgtgatcc agaccgacgc
    22201 cgcgattaat ccgggcaact cgggcggccc cttgctcgat agcgccggcc gattgatcgg
    22261 agtgaatacc gcgatttaca gcccgtccgg ctccagcgcc ggcattggct tcgccgtgcc
    22321 ggtcgatacc gtcaatcgag tcgttccgca gctcatcgct cacgggcaaa ccgttcgacc
    22381 acgcatgggc gtccgcctcc tgagcgagcg gctcaatgcc caactgcttg ccggcctcgg
    22441 cgtcaagggt gtcatgatcg ccgacgtcga ggaaggctcc ggggccgcag ccgccgggct
    22501 gcgaggagtt cgccagactc ccgaaggtga cattctgccg ggagacgtta ttcagaaagt
    22561 cgatgaccgc aacatcgatt cgatcgacgg actactgaat gtcattgaga aacacaaggc
    22621 cggcgacacg gtgagagtaa cgttcctcag agacggcaag acgcagacag tggacgtggt
    22681 gctgcaataa ggagacacca actatgagtg ccaagaagat cctgatgatc gtcggggatt
    22741 tcgtcgagga ctacgaagta atggtcccct ttcaggccct gcaaatggtc gggcacacgg
    22801 tacacgccgt ctgcccgggc aagaaggccg gcgagaaagt ccggacggcc atccacgatt
    22861 tcgagggcga ccagacctac agcgaaaagc ccgggcacaa cttcacgctg aacgcgacgt
    22921 tcgccgggct gtcaccggat gattaccacg cgttggtcat tcccggcggc agggctccgg
    22981 aatacctgcg gctcaatgaa caggtcatca agatcgtcca gcatttcgtc aggacaaaaa
    23041 aacccatcgc agccatctgc cacggggccc aactgctggc cgccgccggg gctctgcagg
    23101 gtaaaacgtg cagtgcctac ccggccgtcg ggccggatat cgccggcgcc ggcggcacct
    23161 acgtcgagat cggcatcgat caggcccacg tctgcggcaa cctggtcacc gcccccgcct
    23221 ggccggcgca cccggcatgg ctggccaagt tcctggaagt gctggggacg aaaatcgagc
    23281 cgtgagcagg cggccactgc caccttcaac aagtgaacgt cgaacgagca ggcttcctgg
    23341 ggcgtcagac cgaatcatca cggcaccgca tatccgcttc tgccgcgatg gatgagcccc
    23401 tggctcctgt ggtataatac tctgcgaggt gaagcatgac ctacaaaggc aaagtgcaag
    23461 gcggtgtggt ggtgctggag ggaggggcgg ctttgccgga aggcgccgag gtggtcgtcg
    23521 agccggtgga ggacctgccc agttgggctg aggtattcaa ggacgtcttg ggcaaggcca
    23581 ccggattgcc gaaggacctg gcccggaacc acgatcacta tctgcatggg gcgcccaagc
    23641 gatgacccct tgctttgccg acagcttcta cttcctcgcc attctaaacc ccgacgacga
    23701 ggcccacaag aaagcagcgg agtgggctgt atcgttgcga cgtccgcttg tgaccacagc
    23761 ctgagtgctc actgaggtag gtgatgcgat ggccgacaca cccaaccgac cgctgtttgg
    23821 gagattcctg gacaacctgc gatctgatcc gcgcgcactt gtggtcagta caaccgacga
    23881 gctttttgat cgcggagttg atctttacga ggcacgcccg gacaagagat ggtccctgac
    23941 agactgcatc tcgttcgtcg ttatgcggga acgcgggatc tcggaagccc tgaccggtga
    24001 tcaccattcg agcaggcggg gtttgtagcc ctgctcaagt aagccggcca ggaggcccac
    24061 ggttgagggc cggcgccgat cagaactgtc tgacaacttg ctgggatcag gtccgaggct
    24121 actcccgtag atcgcccggc ctcgtcagcc gattctgccc cagtagttga tggcgacgtg
    24181 ggctcggttg acgtccgtgt cgacgcggac ggcgatcagg acgtggaaga aaccgcggcg
    24241 gtagtcctcg gggtcgcgaa acgtgctctg caggctcatg ctggagtaat ccttgctgta
    24301 cgtgtgcgaa cccttgcccc gatcggtcca gatgtaggtg accgtcaccg caccggggct
    24361 cactccgccc ttgcggcacg cgccgacgta ccagccggct ttctcgagct tccggacata
    24421 atagtgattc aactgcctgg ctatggcctc acggtctgtc ttctcggtca actgctcgct
    24481 cagatcgacg tcgaactcga tccccagtcc gggctccaca acgtaagaac ctcgctggtc
    24541 gatttcctgc cggattcctt ccatcagaaa ggctgagtcc tcaagctctc gcttaatccg
    24601 tttctcgctg tcggatgcat agttttccga aatacctgag tatgaggttc cgggcatcgg
    24661 atccttgatg tcgttgatgg ccgagatcac aaaggccctt cggttgatga aatcatccac
    24721 ctgggctggg cttggcctgc ggctgggggc gcagccatag agaccacagg aagcgagcag
    24781 caagacaacg attgtcaggc gagttgagct catgtcatcc tccttgaatg ttccggtcat
    24841 tacaggaaag cggtcgcatc ggagaccgcc cctttctgcc ggttgggtcg ctgtccttaa
    24901 gaccacgtgg ataaaccccg tggctcgtca aggctgctga agtccgcccc acctcactga
    24961 accctgaacc ccgaaccctg ctcgccacgc cgaagcctcg gcgaaggcgg gaaccctcac
    25021 accgtcttcc actcatgcag ccactggtag cgggccggca ggtgggcgtg gaagctctcg
    25081 gtgcactcgc gcagggcctg cttgtccttg tcggtcagcg gcttgtcgcg ctcgtaggag
    25141 gccttcacgt tctcatcaag ctgctcggcg gcggtcacac ccggaatgac ggccgagatg
    25201 cgtttttcgg ccagcatggc cttgagcagg ctgctggccc gcgtgtccac cttgccctcc
    25261 agcttggtgg gcgtgccgcc gacaaaagtt gctccagccg cgaaaggctt gatggcgatc
    25321 acgccgacgt ccttcttctg ggccagatcg agcaggctgt cacccttgaa agccgtggtc
    25381 aggaacatgt acgggaacag gatcaccgag aactgtggat agtcgttcaa tacccggcgg
    25441 aagacctccg gcttgtgggc cgagacgccc aggaagcgga ccttgccctg tgtgcgggct
    25501 ttctcgaata cctcgaccac catgtcgagc acgcgggggc tgatttcaag cattgtctct
    25561 tgtttagtct gcccctcgcc ccatgtggcc cccaccggcc gccaaatgtc gagcatgtcg
    25621 gtatcgtagg ccttgagacg gtcatcgatt gccttggcca tgccttcctt ggacaattcc
    25681 ttctcgtatt cgctggtgag cttctccggc caggaggcaa aaccgatgta ccagcggtcg
    25741 cgtttgcctt tcatcgcctt gccgtagagg gcacactcct cgacaatgtt gttgtcgacg
    25801 taattcatgc ccaactcgat ggctcgctca agaaccggca cgcgatcctc aaccgtcttg
    25861 cctccatgac cgccgagact gatctctgaa atcatgaggc cggtcttgcc caatcgccga
    25921 tatcccatct tcgggttgta gttgagaatc ttcgacgtat cggccggtcg agtctggacg
    25981 gcggacgcct ggcccgccag ggtcaccgag gccatcgcca ccgaagtggt cttcaggaac
    26041 tcacgccggg tcggtttgct ctgactcatg ccctgttctc ctggctcact tgccgtagcg
    26101 cttgcggaat tcctcggcgg ccttggctcg ccgctgcgtc tcggccggat ccagcttgtc
    26161 aatgtaccaa ctgtggttgg gggaactcaa cacctcatcc agcctggctt ggagcttctt
    26221 ggcggcttcg gccttggcag catcgctgga gttcgcgttc gcgtgggcta attcccgcaa
    26281 ctcggggacg tactcgctgt agaaatgctt ggccacctcg tatgtgccgt gccagtgggt
    26341 gtaatccggc cccatcatgg aggccccgtg ccgggcccgc cgcccttcat ggtgccagat
    26401 ctcgtaccag acgaagtcga tcttgttggc gaattcaacc ggcaccagca acggcttggc
    26461 tagttcgtac agttcgaggc cgggcttggc gaacttgtca tgatacagct cgaccaggct
    26521 gtcatactga acgtagaagt tgtccaccca ctgcttctcg tggcagttga ggcagacgtt
    26581 ttgcatgttg gcgcggcggg tcttccagtc gatgtcggcc ccgggcagcc ccatcttctt
    26641 gtcgctgacc tcgggtcgga tcgaaatggc cggccgattg ttccaactga tccgcaggcc
    26701 gatatcgtgg ttcaggggtt gcgtcttggt ggcgctcatg tggcaggtgg cgca
//
LOCUS       DAOTPW010000047        26481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1912_length_26481_cov_5.312571, whole genome shotgun sequence.
ACCESSION   DAOTPW010000047 DAOTPW010000000
VERSION     DAOTPW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26481
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1912_length_26481_cov_5.312571"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="P5316_06230"
     CDS             complement(<1..703)
                     /locus_tag="P5316_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84592.1"
                     /translation="MDDLRKTKKQLVDELHALRARVALLEREASAREVLPLERDEQPF
                     EYLPRLSRDSAPADGSSVAPGESGCGDEGRACAPAEILCIAPSPAMESLAQVCQDLGH
                     HCTRVTDWDSLRNLQGRFHLAVVAVKPGGHALFNGPTLHDLMVHPPGWPMVERGLLLG
                     NMDGLRGHVHAYEQRASGYCQIGDPERPMSPDLLARIRCAIVDVLRLPAYEPVNFFDE
                     VDDSVIHSVIESSQAP"
     gene            1104..2042
                     /locus_tag="P5316_06235"
     CDS             1104..2042
                     /locus_tag="P5316_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family transcriptional regulator"
                     /protein_id="HRR84593.1"
                     /translation="MAIDCRQFVEEQIRELRAKVGKETAITALSGGVDSSVVTVLGHK
                     ALGSRLKTVFVDNALMRENEPQRVVEIFGKMGIKVDLIDAREEFLSALAGLTDPEQKR
                     NAITNTFYSRVFGRLVRESGARFLLHGTILTDIEETVAGIKRQHNILAQLGIKPEEAY
                     GYTVLEPLSTLRKDGVREVARFLGLPNEISERMPFPGPALAARIVGEVTRERLDTVRA
                     ATAIVEEELINCGAFQYMAVLLNDKATGIRDNKREFGQIIVVRCMESVDAREATVTRP
                     SWETLDRISQRITQIKGVNRCLYDLTPKPPATIEYV"
     gene            2077..5310
                     /gene="carB"
                     /locus_tag="P5316_06240"
     CDS             2077..5310
                     /gene="carB"
                     /locus_tag="P5316_06240"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRR84594.1"
                     /translation="MPRRNDIHKVLIIGSGPIVIGQACEFDYSGTQACKALRKLGYQI
                     VLVNSNPATIMTDPGMADVTYIEPLTVETLSEIIAKERPDALLPNLGGQTGLNLTAEL
                     AKAGVLDQYGVTVIGVQVDAIERGEDRTAFKNTMNRLGIDMPKSEAVTSVEEAERVAA
                     GLGYPVVIRPAYTLGGTGGGLVYNVEELRTVAGRGIAASLIGQILVEESVLGWEELEL
                     EVVRDAKGQKITVCFIENVDAMGVHTGDSYCTAPMLTIAPELQARLQKYSYDIVDAIE
                     VIGGTNVQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIAMVSAMLAAGLTLDEI
                     PYWRDGTLEKYTPSGDYVVVKFARWAFEKFKNAQDKLGTQMRAVGEVMSIGKNYKEAF
                     QKAIRSLENGRHGLGFARDFNRRSLSQLMDMLREPSSERQFIMYEALRKGASVDELYG
                     LTFIKPWFIQQMKELVELEEEILKYKGSLLPDTLLTKAKKDGFADKYLAKLLGVAESQ
                     IRNQRLRLGVVEAWDAVPVSGVENAAYYYSTYNGPDRVSTTHRKKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALRDMGFETIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIYEK
                     EKPVGVICQFGGQTPLNISQELAAAGVNVLGTSPDMIDLAEDRDRFRQMMDRLGIPMA
                     PSGMASTLQQALAIAQRIGYPLMVRPSYVLGGRGMEIVYDEEMLKQYVAAAVDVTPER
                     PILIDRFLENAIETEADAIADGAQTFVPAVMEHIELAGIHSGDSACVIPPVSIPAKHL
                     DTIYEYTQRIARELNVVGLMNMQYAIANDVVYVLEANPRASRTVPLVSKVCNIQMARI
                     ATQLMMGRKLADLKLTPREIRHYGVKEAVFPFPMFPEVDPVLGPEMRSTGEVLGLADS
                     FGMAFYKAEEAAKSRLPTEGTVLISVSRKDRPAVLKPAKRFQELGFKIRATEGTHAFL
                     TTNGVRCEPIKKIHEGRPNIVDALMNKEIHLVINTPVGKLSQHDDSYIRKSAIKYKIP
                     YITTISAALAAVEGIAARRNARGTVKSLQEYHADIAKMPVARRGGRQ"
     gene            5307..6071
                     /gene="hisF"
                     /locus_tag="P5316_06245"
     CDS             5307..6071
                     /gene="hisF"
                     /locus_tag="P5316_06245"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRR84595.1"
                     /translation="MNYRRIIPCLDTRNGRLVKGVHFVNIKDLGDPAENGAAYSQAGA
                     DELVLLDITATLEGRKTLMDAVKRTVEHITIPLTVGGGISEIRHIQELFDAGVSKASI
                     NTAAVRNPNLVNEAVREFGGERITIAIDTRKSASCPSGFEVMVSGGTKPTGIDAVEWA
                     RKVEDLGAGSILPTSMDADGTQAGYDIPMTRAIADAVKVPVIASGGAGKLEHLYEAIA
                     DGHADAVLVASIAHYGTFTIRQMKDYLAQRGVPVRQ"
     gene            6267..7991
                     /locus_tag="P5316_06250"
     CDS             6267..7991
                     /locus_tag="P5316_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR84596.1"
                     /translation="MLPVRHWICFREPMRFSIGSPMALALILTASAAGQPIIIPAGGF
                     WMGRSEPEPPDCSPPGTCYYDDETPAHWVELPEFQISPYEVTNAEYATFLNAVGVTAD
                     AEGRPYIDAADPQIRIHHNGSAWVPDEGWEDHPMREVSWYGADAYCRWAGGRLPTEAE
                     WEKAAGWDEAAQHARKWPWSDTWSCYCSSWWCNAPYYGGPTTYPVGSFPAGVSPYGLF
                     DMAGNVWEWTMGGYRSYAGGPLVFEDDSREVQRGGSWTNSDYNVRCATRSPLPRYITD
                     ANLGFRVCYGPEPSIPPVVINPREQYSEWLEDFTAYTPMDEIYTWWVYGTGTRFLIEA
                     TNGWVLASLRNTTDPKDVGGEVMAMIRRDTGDLFAPGDYVDITVRLRYERGDRDYART
                     SVGVAWGDERTIVPGGRGADENFGYPWFLVANNSDEPGVWHEVTLRQVRWGEGKLCIG
                     FGLWGNLSSLASPPIVGQHRMWVDWVRVRHSLPVTALADLDSDGDVDAEDVGTFKSCL
                     AGPGLRPAGYCARCDFDRDQDVDQVDFGYLQRCISGSTIQADPLCIPPAPAFTGRAIL
                     PANDVLPP"
     gene            8228..9397
                     /locus_tag="P5316_06255"
     CDS             8228..9397
                     /locus_tag="P5316_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR84597.1"
                     /translation="MPRTETLSPRQRVRLALNHEIPDRVPIDLGGFQTGIHKDAYVAL
                     IEHLGIHDSISIIDAVQQLARPCEEVLQRFHVDTRYLCAHAPDGFKGGIIQNRRDGRL
                     WHDLRDEFGVIWSMPDDQPLFMDISYHPLARASTADVASYPFPRGNDPTRFTGVREQA
                     LKLRNETDYAVCTGIGGVVYEYCWYMRGLEQWFIDMIENPAFCEVLLDRMLQYWKDFY
                     TGFLGQVGDLVDVVMIGDDLAGQRGPLFDPAFYRRVVKPRQKALVQHIKRLTQAKVWY
                     HTCGACSLYIPELIDNGVDVVNPVQIGLEDMDPTSLKERFGSRISFWGGGIDAQHVLP
                     FASPAQVREHVRANIAAFMPGGGYVFNNVHNIQHGVPPENIVALFDAAYEFGFYE"
     gene            10211..11041
                     /locus_tag="P5316_06260"
     CDS             10211..11041
                     /locus_tag="P5316_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRR84598.1"
                     /translation="MTRRLRLGLDTYSLHQRLTAKDPRNRKDLWWVLDQLEPCGLEGV
                     QIDPSHFPGDDEKTLERLASVVGPRGHYVEFGMGGWDPTRLEHRGKLTVRFGGRAVRT
                     FCGGETTSHDKIAEYIKWAPPALRQAAEAADRYGFDIAVENHGDFTSTQLKALLDAVD
                     HPRVGACLDTGNSLFRKEDPIHCARVLAPYAKSMHLKDWTMSFRPDGTPEWKEAVLGT
                     GQVPVKEMLRIAIEANPNLYIALEAPVRPSEDETETVDREWQFFKQNARVARELLADL
                     "
     gene            11083..12420
                     /locus_tag="P5316_06265"
     CDS             11083..12420
                     /locus_tag="P5316_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84599.1"
                     /translation="MNHKMTRRRFLRSNVATAVGAGLAGRLVRGENSNPTTRPTDSLS
                     VGIIGCGDRGKYLTYVCQIVPGIRVAAICDVNRDRLAKVHQQLGGQADTHHDYRRILS
                     NKSIDAVVIATNAHWHARIAIDACEAGKDIYLEKPVATSIAEGQAIIKAARRYNRLIQ
                     MGMQQHSWEHYREAVEIVRSGRLGTVSHVHIWDVENAWPGFGMPPDGPAPPELDWEFW
                     LGPSPLVPYNPNRYTRAEWFFDYDGGWQVAWGAHHFDIVHWAMGVTAPVAATGSGGHF
                     AFKDNREWPDTFNGTCEYPSGPVAKNGFLVTYTMRHGSGRLIEGRAHGKAFHGTDGVL
                     IVDRSGYEVLSENRDGARMVAEEKVASYTRSHDVVQNHVKAFLECVRARRSPEADVLV
                     GHRATNPGHLMNIAWRVGRRVRWEGTTEQVVEDSEAAKWLVKTYRRPWALRES"
     gene            12450..13595
                     /locus_tag="P5316_06270"
     CDS             12450..13595
                     /locus_tag="P5316_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84600.1"
                     /translation="MMTADVTSRGRSAVGLLVTGLTVLGWAGAGKAEQPRRTEVAVRG
                     DAFLINGRPTYEGRIWQGHKIEGLLLNSRMVQGVFDDLNPDTRARWTYPDTGRWDPDR
                     NTREFIAAMPQWQAHGLLAFTINLQGGCPQGYCKEQPWHNSAIEPDGSLRNDYMRRLE
                     RILDKADELGMVAILGLFYFGQDQRLADEAAVVRASDKVVDWLADRRYRNVLIEINNE
                     CNVNYDHAILRPDRVHELIERVRQRSDPVRRLLVSASYGGGAVPNQNVVHASDFLLLH
                     GNGVGDPKVIAWMVRETRKVPGYRPMPILFNEDDHFDFDKPVNNFVAAVAEYCSWGFF
                     DPGRGNYRDGYQCPPVNWGINTDRKRAFFEKLSEITGRHPLTSRPAR"
     gene            13665..14813
                     /gene="rlmN"
                     /locus_tag="P5316_06275"
     CDS             13665..14813
                     /gene="rlmN"
                     /locus_tag="P5316_06275"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRR84601.1"
                     /translation="MTSNHRKQHLLGTPLTGFATFCAEQKWPVYRARQVYQWVFDRGV
                     TDFEAMTNLAKTERAKLAASWTILTGKELRRQESEDGTTKLLLEWTDGATSEYVFIPD
                     ETRRTACISSQIGCPVGCTFCASGMGGLQRQLTAGEIVEQAARIRALCRQPPEDRRFG
                     EDTRLSNIVIMGIGEPLANYENVLQAVRMINADWGMNIGARKITLSTVGLPKQIRRLA
                     EEDLQINLAISLHAPTDSLRRRLIPWANRVSVDELVSAANFYFEKTGREVTIEYLLLG
                     DVNDRPRHAADLASLCRRMRCNVNLIRYNPVAGLGFERPTSAAAHHFVEQLRARGINA
                     HVRKSRGCDIDAACGQLRRTHRGPGSPGAVDKPASPGSNMTAPDGAIE"
     gene            14936..15760
                     /locus_tag="P5316_06280"
     CDS             14936..15760
                     /locus_tag="P5316_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR84602.1"
                     /translation="MEPLQIGVCSWSLKMPDIGDALVAVKHELGLNLVQIGFWDEGFK
                     QTDRIIKLIERTGLEVSATCIGFEGEDYSSIQRIAETGGFKPDNEWPRRLAKTKAFAD
                     FTAALNVKMLACHIGFVPHDKSESVYAVMVDRIKQVCDVLAHRDLTLVMETGQERAEN
                     LLEFIDAVERDNIRINFDPANMILYGVGDPLQAVDILKDKIVHCHMKDAVWSREPTKI
                     WGEEVVLGTGQANIPKLIAKLRSYGYTGPLVIEREAGNQRIADIKTAIGLLEKVVG"
     gene            15923..18211
                     /locus_tag="P5316_06285"
     CDS             15923..18211
                     /locus_tag="P5316_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRR84603.1"
                     /translation="MSNPDRHQSTPADENVQLRERLHRLEHPGEEKEVFVHLARRLAG
                     ATSADAVMAAAQEASERLLAWDFFYLAHRLPESNQFRIAGVIDTIEGRRHTFPEQRDA
                     EATFSPPVREVLAGKRLLVNRREGRTTPVMRPLGSGRPSASLMFVPVQCEGQVIGLLS
                     AQSYVPNQYGESDLQILQDVADAVAPAMERVRAEESLRSREQLLRLERDLAVSLGAAR
                     DVHEALDRLLETSLQIEGIDCGGAYLVDPATHDLILARYRNLSAGFADGVKHLARDSW
                     QAALVLKAKTVYMSTAELHSIDAACRDEGLMSVAVAPVAQSGNLIAVLNLASRRLNEI
                     PPHARHAIEAVAAQMGTALIRIRAEQDLREARDQLEQRVEQRTAELREANARLEAEII
                     EHQKSEQALLEKEAQLRSLNENLAEGMVYQINSGQDGQKRQFTYLSPAVEHLHGLRVE
                     DVLANSTLIYDQVIEEDRSLLAEQETRAFATRTTLDVDVRVRLPSGEIRWRRFISSPR
                     VVPDGTLMWEGIELDITRRKKAEEALLRKERELRLLSAEANRRLEEERARVSRELHDE
                     LGQMLTALNLNLRWLGRRIGDVGDDVVERIDESIQYVSRMITSVRTLSRSLRPAALGH
                     EGLVEAVRSHMAEFEQYAGIRCRVEVVPPNLAVSEPLATTAFRIVQEALTNVARHSKA
                     TFCRVNIRAVDDLLVLEIRDNGVGLKEQEPTDAISLGIPGMRERAETIGGSLVVANNP
                     EGGVCVTARLPRQRRDHSPLGS"
     gene            complement(18238..20346)
                     /locus_tag="P5316_06290"
     CDS             complement(18238..20346)
                     /locus_tag="P5316_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR84604.1"
                     /translation="MMPRAVQTVEPRARDVWRRRFRPRPWPAAWAIALTFLANVPVLG
                     QSRPASAPAEDPVGQAEALDRFSLPQDGLNDMEVFNLDIPEVDVVQTATRSPKRIMDL
                     PYAVSVVTADDIRRSGARSVPDALRLLPGVDVADLSWGYSGVSTRGAHGLTADQVLVL
                     MDGRQMFDPILGATFWYFWPIQLEDIERIEVLRGPAGVTWGANALNGVINIVTKDPAD
                     QVGLTSTVSGGSRGAHKEHLGYGYADEKLKLRVSGEYESSDGFRKGGSILQQLDDDFL
                     GGRTGVHMVYDATPKDVFTLSGGNSVAGGGNPTSPSFFPPDKLSPRSQTAFLLGKWDH
                     RVARDNQFGINAYVNDYHFEPGAKTIDYRYQQIALEFNHNFKLGDNHTLTWGLDSRTD
                     LIDGTNSDPHLMTRDYVSSATIGLYVQDEWRFAPRWALELGARIDYEFYGGFQPSGRI
                     ALSHKFSDGSLAYGAVSRAFQMPPVGGRFADFVLYSGLIHQTADPETDPQTVIAYELG
                     YRKKFLSDRLTTNFNLFWHDMDDVITATTMEFGPPGLLQAKYDNRASAAMYGFEFEAK
                     YAATKQLTLLAHYTYQWMDWFGHVPYTDKDFITPPEHKFMIGAQYDPVKDLHLSAYLH
                     YVDTVKAPNGTMALLPMQIPPYLRLDLRAEYEFWKKQAAVAVGVRNLLDPDHPEGGTT
                     FLNNAEVPRMVYAEFRLSLK"
     gene            complement(20383..21414)
                     /locus_tag="P5316_06295"
     CDS             complement(20383..21414)
                     /locus_tag="P5316_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRR84605.1"
                     /translation="MTTVILFLLALVAGHNSQATHGAESARITILAAPQAIYRKTIDS
                     IETAAKQKKWSYVMVELPAPPEQPQPEDSVDSDKASASPAPASRPASSGVSLQDVRSR
                     LVQTRPAVVIAVGTEATILALESVPDAAVVFCMVPNALDASFLAEDSRHRARVAGVTT
                     DISPVEQIAWVTQMDEGVKNIGVPFSSRTRRTVEALRKAGQDRGMTITPIETSKNDFL
                     KAARLLDSNRCDGVIMVPDADVYNTATAQRLLLWGIRARKPVWAFSSKIVKAGAFAGW
                     QADDESIGRQTVDLADKIAGGTKPASIGVVYPAKVICSINHRSTEMIGISLADDILAK
                     IPEQYGRND"
     gene            complement(21961..23679)
                     /locus_tag="P5316_06300"
     CDS             complement(21961..23679)
                     /locus_tag="P5316_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRR84606.1"
                     /translation="MVRIMAYWNRRQAGRCRWGVVLVAAAVAVVVLPCWVCAEQSRAS
                     KPAAIGTRFGKDIPKYRIYPLRNGRCEIAGNDAFEGGDPAQSYEYVLYVWLILGGDKP
                     MLVDAGLSDVAEMNRGAAAVLKKPITQDPTETVAAQLARFGLKPAEIGHVFITHLHFD
                     HVDGLDAFTNARIYIGKQEWQLATANDCKGSWGHGRIMFMLRDNPEWNKRLVLVEDEE
                     VLPGIESFFVGGHTLGSTAYQVNTAHGRTVLTGDTVSLLANIEKDIPVGVRMDLDQCR
                     AGMKKIRERADIVLPSHDPTTPRRWPPVAPGLPRYTIRAIKVGQCEVREYISFQDSEG
                     QDTRMYNLYVWAVLGGPHPILVETGPNPKHVGDFNRATAKYIPGGVRQLPEEDTLIAL
                     KRVGIDPSDVSHVIVTHLHADHYDYYPAFPNARLVVNRREYEQTRGDADNPEDRGHLN
                     PEVFAAIRSHPEKLILAEDQEILPGIRTVPLGCHTPGSQGVLVQTHVGPVMLTGDVVY
                     LYENIERDRPARSPDPRVCQEAMAKIRTLADIVLPAHDPLTLVRWPGGVIGAEPGSVA
                     TRQAAK"
     gene            24427..25185
                     /locus_tag="P5316_06305"
     CDS             24427..25185
                     /locus_tag="P5316_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR84607.1"
                     /translation="MKNFLTIAGGLLAVLAFGMPATAAVVWSDGVNLPYSSATVDIEG
                     EFDNEAAWAGGDFKVLNGTASQVPAGWKFAQQSWNNWGNWAVSSSTNLTEGNAALKMT
                     PGGGDWNDIFAGTFITGLTVGQAYTLTFDFQADVGFDKGNPGLKTGDDHQPSASFNVM
                     NVPDNPIGWGATEDYPGHLTGSTWIGLGNWDGNYHQQSRDFVATADSMAFVIKIRSMN
                     SPGSVRIDNLVVTPEPAAMILLALGGLACLYRRR"
     gene            25622..>26481
                     /locus_tag="P5316_06310"
     CDS             25622..>26481
                     /locus_tag="P5316_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84608.1"
                     /translation="MTQRARVSRRTLLKRAAGALAVPYFVPAWALGRGGWLPPSERIT
                     VGFIGIGNMGGGHFDGFLEDKQLQVLAACDVRRDVRERRKEQADKKYAADRQAGDYKG
                     CAAYNNFEDLLARDDIDAVLIAVPDHWHAIIACAAARAGKDIYCEKPLTLTIKEAWDL
                     VDTVRRYGRVFQTGSQQRSDREFRLACELVRSGRIGKLQTVTVNIGEPSQEAYLPEQP
                     VPEGFDWDRWLGPAPWQPYNAERCSGNYGGGWRKIRDYSGGMTTDWGAHHYDIAQWGM
                     GMDGSGPVEI"
BASE COUNT         5269 a         8390 c         8017 g         4805 t
ORIGIN      
        1 tcggagcctg gctgctttcg atcaccgaat gaatgacgct gtcatcaacc tcgtcaaaga
       61 agttgaccgg ctcatacgcc gggaggcgca gaacgtcaac gatggcgcac cgtatcctgg
      121 ccagaaggtc gggagacatg ggccgctccg ggtcgccgat ctggcagtag cccgaggctc
      181 gctgctcgta agcatgcacg tggcctcgca gtccatccat attcccaagt agaagtcccc
      241 gctccaccat cggccaaccc ggcggatgca ccatcaggtc gtgcagggtc ggaccgttga
      301 aaagcgcatg ccccccgggc ttgacggcca ccacggccag gtgaaaacgc ccttggaggt
      361 tccgcaggct gtcccagtcc gtcacacggg tgcagtggtg ccccaggtcc tgacagacct
      421 gcgccagcga ttccatcgcc ggcgatgggg cgatgcagag aatctcggcc ggggcgcacg
      481 cgcggccttc gtcaccgcag cccgattccc ccggggcgac ggatgacccg tcggcgggcg
      541 cggaatcgcg agacagccga ggcaggtact cgaatggttg ctcgtctcgt tcaagaggaa
      601 gcacttctcg agcggaagcc tcccgctcca gtaaggccac tcttgctcgc agcgcgtgca
      661 actcatccac aagctgcttc ttggtcttcc tgaggtcgtc catatgatgt ctcaccagcc
      721 ttccgcaggg tcctcagaac ctccggacgc cgattccggc tgtccagggc cagacatgtc
      781 gagacagtat gcgttccccc ctggggtccg caatcgatga ccccccgcta acgtgcgaca
      841 cgtgcataga ctattgtacc ttttcacgac gaaatagaaa ggtacacacg aaggaggcgt
      901 ttggggcggg cgcacgccgc cgcgcgccct tccgcgccgg gacccggaac ccgcgcgtcc
      961 tttggcaaat aaaaaggcgg ggctttgccg ggtttgacat ggattcctaa tctcattata
     1021 tcaaacgccc aatctaaagc ctcaggttgt ctcgctggcg cgatcctggt cgtctccggt
     1081 gccctgccgg gaaggagagt gcgatggcta tcgactgtcg acagtttgtt gaggagcaga
     1141 ttcgcgagct gcgtgcgaag gtcgggaaag aaaccgctat cacggcccta agcggcggcg
     1201 tcgacagctc cgtggtgacc gttctgggcc ataaagcctt gggctcccgg ctcaagacgg
     1261 tcttcgtgga caacgccttg atgcgcgaga acgagccgca gcgggtggtt gagatcttcg
     1321 gaaagatggg gatcaaggtc gacctgatcg acgcacggga agagttcctc tcggccctcg
     1381 ccggcctgac cgatccggaa cagaagcgga acgccatcac caacaccttc tactctaggg
     1441 tgttcggccg gctggtccgg gagtccgggg ccaggttcct tcttcacggg acgattctga
     1501 ccgacatcga ggagaccgtc gccggaatca aacggcagca caatattctc gcacaactcg
     1561 gaatcaaacc ggaggaggcc tacggttaca cggtgctcga acctctgagc acgcttcgga
     1621 aagacggcgt gcgggaggtc gcgaggttcc tgggcctgcc gaacgagatc tccgagcgaa
     1681 tgcccttccc gggaccggcc ctggcagcgc gaatcgtcgg cgaggtcacc cgcgaacggc
     1741 tcgacaccgt tcgagccgcg accgccatcg tcgaggagga actgatcaac tgcggggcct
     1801 tccagtacat ggctgttctg ctgaatgaca aggccaccgg catccgcgac aacaaacgcg
     1861 agttcggcca gatcatcgtc gtacgctgca tggaaagcgt cgacgcccgc gaggccaccg
     1921 tgacgcggcc gtcgtgggag acgcttgacc ggatttcgca gcggatcacg cagatcaaag
     1981 gcgtgaaccg ttgcctgtac gacctgacgc ccaaaccgcc ggcaaccatc gagtacgtat
     2041 gaccttatcc aggatcggga ggcgggaact acccagatgc cgcgccgcaa cgacattcac
     2101 aaagtgctca ttatcggctc aggtccgatt gtcatcggcc aggcctgcga attcgactac
     2161 tccggcaccc aggcgtgcaa ggcccttcgc aagctcggct accagatcgt gctggtaaac
     2221 tcaaacccgg caaccatcat gaccgacccg ggcatggccg acgtgaccta catcgaaccg
     2281 ctcaccgtcg agaccttgtc ggagatcatc gctaaagagc gccccgacgc cctcctcccc
     2341 aacctcggcg gccagaccgg ccttaacctg accgccgagc tggccaaggc cggcgtgctg
     2401 gaccagtacg gcgtcacagt gatcggtgtt caggttgacg ccattgagag gggcgaagac
     2461 cggacggcct tcaagaacac catgaacagg ttgggcatcg acatgcccaa gagcgaggcg
     2521 gtcacctccg tcgaggaggc cgaacgcgtg gccgccggcc tgggctatcc ggtcgtcatc
     2581 cgccccgcct acacgctggg cggcaccggc ggcgggctgg tgtacaacgt cgaggaactc
     2641 cgcaccgtcg ccggccgcgg gatcgccgcg agcctgatcg gccagatcct cgtggaagag
     2701 tcggtactgg gatgggagga gcttgaactc gaggtcgtcc gcgacgccaa aggccagaaa
     2761 atcaccgtgt gcttcatcga gaacgtcgat gccatgggcg tgcacaccgg cgattcgtac
     2821 tgcacggccc cgatgctcac gatcgcaccg gagctgcagg ccaggctcca gaagtattcg
     2881 tacgacattg tcgatgccat cgaggtgatc ggaggcacga acgtccagtt cgcccacgac
     2941 ccgaagaccg gccgcgtggt ggtcatcgag atcaacccgc gaacgtcgag atcgtcggcc
     3001 ctggcgtcca aggccaccgg ctttcctatc gccatggtgt cggccatgct cgccgcgggt
     3061 ctgacgctcg acgagattcc ctactggcgc gacggcacgc tcgaaaagta caccccctcc
     3121 ggcgactatg tcgtggtcaa attcgcccgc tgggctttcg agaagttcaa gaacgcccag
     3181 gacaagctcg gcacgcagat gcgggcggtc ggcgaggtca tgagcatcgg caagaactac
     3241 aaggaggcct ttcagaaggc gattcgttct ctcgaaaacg gtcgtcacgg cttgggcttc
     3301 gcccgagatt tcaaccggcg gtcgctgtcg cagcttatgg acatgctgcg cgaaccttcg
     3361 agcgagcggc agttcatcat gtacgaggcg ctgcgaaagg gcgcctcggt cgacgaactc
     3421 tatgggttga ccttcatcaa gccctggttc attcagcaga tgaaggagct ggtcgagctg
     3481 gaagaggaaa tcctcaagta caagggcagc cttctgcccg acacgctgct gaccaaggcg
     3541 aagaaggacg gcttcgcgga caagtatctt gccaagctgc tgggcgtcgc cgagtcgcag
     3601 attcgcaacc agcggcttcg tctcggcgtt gtcgaagcct gggacgcggt gccggtcagc
     3661 ggagtcgaaa acgccgcata ctactactca acatataacg gccccgatcg cgtctcgacc
     3721 acccaccgca agaaggtcat ggtgctcggc ggcgggccga accgcatcgg ccagggcatc
     3781 gagttcgact attgttgcgt ccacgccgcc ttcgccctcc gcgacatggg ttttgagacc
     3841 atcatggtca actgcaaccc ggagaccgtc tccaccgact atgacacctc ggacaagctg
     3901 tactttgaac cgctgacggt cgaagacgta ctgagcattt acgaaaaaga gaagccggtc
     3961 ggcgtgatct gccagttcgg cggccagacc ccgctcaata tctcccagga gctggccgcc
     4021 gccggcgtca acgttctggg caccagcccg gacatgatcg acctggccga ggatcgcgac
     4081 cggttccgtc agatgatgga ccgactgggc attcccatgg ccccgtccgg tatggccagc
     4141 acgctccagc aggctttggc catcgcccag cgaatcggct atccgctcat ggtccggccg
     4201 tcttacgtgc tcggcggccg gggtatggaa atcgtctatg acgaggaaat gctcaagcaa
     4261 tacgtcgccg ccgcggtgga cgtcacgccg gaacggccga tcctgatcga ccgattcctg
     4321 gagaacgcca tcgagacgga ggcggacgcc atcgccgacg gcgcccagac cttcgtaccg
     4381 gccgtcatgg agcatattga actggccggg atacattccg gcgattcggc ctgcgtgatc
     4441 ccgcccgtca gcatccccgc caagcacctc gacacgatct acgaatacac gcagcggatt
     4501 gcccgtgagt tgaacgtcgt cggcctgatg aacatgcagt atgccatcgc caacgacgtc
     4561 gtttatgttc ttgaagcgaa cccgagagcc tcacgcaccg tcccgctggt ctccaaagta
     4621 tgcaacatcc agatggcccg catcgccact cagctcatga tgggcaggaa actggccgac
     4681 ctcaagctga cgcccaggga aatccgccat tacggcgtga aggaggcggt cttccccttc
     4741 cccatgttcc ccgaagtcga tccggttctt ggccccgaga tgcgctccac cggcgaagta
     4801 ctcggcctgg cagacagctt cggaatggcc ttctacaagg ccgaggaggc tgccaagagc
     4861 cggttaccga ccgaagggac ggttctcatt tcggtttcac ggaaggaccg ccccgccgtg
     4921 ctcaaaccgg ccaaacgctt ccaggaattg ggcttcaaaa tacgggcgac cgaaggtacg
     4981 catgctttcc tgacgaccaa cggcgtccgg tgcgaaccga tcaagaagat tcatgagggc
     5041 cggccgaaca tcgttgatgc tctgatgaac aaggaaatcc acctggtcat caacacgccg
     5101 gtcggcaaac tcagccagca cgacgacagc tacattcgta aatccgccat caagtacaag
     5161 attccctaca tcaccacgat cagcgccgcg ttggccgccg tggaaggaat cgctgcccgg
     5221 cgaaacgcac gcggcacggt caagtcgctc caggagtatc acgccgacat cgcaaaaatg
     5281 ccggtcgcca gacggggagg tcgccagtga attatcgtcg tatcattccg tgtctggata
     5341 cccgcaacgg ccgcctggtc aagggtgttc atttcgtgaa tatcaaggac ctcggtgacc
     5401 ccgctgagaa tggggcagcc tacagccagg caggcgccga tgaactcgtc cttctggata
     5461 tcaccgccac gctcgaaggc cgtaagacgc tcatggatgc cgtgaaacgc accgtcgagc
     5521 acatcaccat ccccctgacc gtcggcggcg gcatttccga aatccggcac atccaggagc
     5581 ttttcgacgc gggcgtaagc aaggcgtcga tcaacactgc cgcggtacgc aacccgaacc
     5641 tcgtcaacga ggccgtcaga gagttcggcg gcgagcggat taccatcgcg atcgatacac
     5701 gcaaatcggc gtcctgcccc tccggttttg aggtcatggt cagcggcggg acaaagccga
     5761 ccggcatcga cgccgtcgaa tgggccagga aagtcgaaga tctcggtgcc ggtagcatcc
     5821 tgcccaccag catggacgcc gacggcaccc aggccggcta tgacattccc atgacccgcg
     5881 ccatcgccga cgcggtcaag gtcccggtca ttgcctcggg aggcgcgggc aagctcgaac
     5941 acctctacga ggccattgcc gacggccacg ccgacgcggt gttggtcgcg tcgatcgccc
     6001 actacggcac gttcacgatc aggcagatga aagactatct cgctcagcgg ggtgttcctg
     6061 tcaggcagta gccggtccgg tcgtactcgg ttcccccaga cggggtgatc cggttcgatc
     6121 tgcgatgccc tgtcattttt cgcgaaagcg ccaatcctgc cgctaccatc aaggctgctc
     6181 tgacggtaca atgaacgcaa tgtgatcgcc gtgcaggctg acgcggcagt ggtcaggccc
     6241 gatcgaggtg gctggaggga aacggcatgc ttccggtgcg gcattggatc tgtttccggg
     6301 aacccatgcg gttctcgatc ggcagcccga tggctcttgc cctgatcctg acagcttccg
     6361 ccgccggcca accgatcatc attccagccg gcggcttctg gatgggacgc agtgagccag
     6421 agccacctga ctgctcgccg ccgggaacct gctactatga cgatgaaacc ccggctcact
     6481 gggtcgagtt gcccgagttt cagatcagtc cttacgaagt caccaacgcc gaatacgcca
     6541 cgttcttgaa tgccgtcggc gtcactgccg acgctgaggg ccgtccctac atcgacgcgg
     6601 ctgacccgca gatccggatt caccacaacg gatcagcctg ggtgccggac gagggttggg
     6661 aagaccatcc catgcgcgag gtctcctggt atggtgcaga cgcttattgt cgctgggccg
     6721 gcggacgttt gcccactgaa gcggagtggg aaaaggccgc cggctgggac gaggctgctc
     6781 agcacgcccg aaagtggccg tggtccgata catggagctg ctattgcagc tcatggtggt
     6841 gcaatgcccc gtattacggc ggcccaacaa cctatccggt gggttccttc cccgcaggcg
     6901 tcagcccgta cggccttttc gacatggccg gaaacgtctg ggaatggacc atgggtggtt
     6961 atcgctccta tgccggcggg cccctcgtgt tcgaggacga cagtcgcgag gtccagcgag
     7021 gcggcagttg gacaaacagc gactacaacg tccgttgtgc gacccgaagc cctttgccca
     7081 gatacatcac cgatgcgaat ctcggttttc gcgtttgcta tgggcctgag ccgagcattc
     7141 ccccggtcgt gatcaaccca cgtgagcagt attccgaatg gctggaggac ttcaccgcct
     7201 acacgccgat ggacgagatc tacacctggt gggtctacgg tacgggaacg cgcttcctca
     7261 ttgaggcgac caacggatgg gtcctcgcct cgctcaggaa caccaccgat cctaaagacg
     7321 tcggcggtga agtgatggcg atgatcaggc gtgataccgg ggatctgttc gctccgggtg
     7381 attacgtgga catcaccgtg cgcctgaggt atgagcgcgg cgaccgggac tatgcccgca
     7441 cgtcggtcgg tgtcgcttgg ggcgacgagc gaacgatcgt gcccggcggc cgcggggccg
     7501 atgagaactt cggttacccg tggtttctcg tggccaacaa ctcggacgag cccggtgtct
     7561 ggcacgaggt cacgctcaga caggttcgat ggggcgaggg caagctctgc atcggcttcg
     7621 ggctttgggg gaacctcagc agcctggcca gcccccccat cgtcggccag caccgcatgt
     7681 gggtggattg ggtgcgggtc cgccactccc tgccggtgac ggctttggcc gacctcgatt
     7741 ccgacggcga cgtcgatgcc gaggacgtcg gcacgttcaa gtcgtgcctg gccggcccag
     7801 gtttacggcc ggcaggatac tgcgcccgct gcgacttcga ccgggaccag gacgttgacc
     7861 aggttgattt cggttacctc cagcgctgca tcagcggctc cacgattcag gccgatcccc
     7921 tgtgcatacc tcccgcgccg gctttcaccg gccgggcgat cctccccgcg aatgatgttc
     7981 tgcccccatg acaaccgcgc cgcgaccacg atgcgggtag gcgacaacgc atcatctctc
     8041 atgcggacag taccgtcctt cggctcgcga gatcctcgac cgttgcaacc tcgccgactc
     8101 cgccgcataa tcctgcgatc gtgagcctgc ctcgttcgcg tgcggcgccg gcgacgtgcc
     8161 ggcccgatga caagcgcgag ccggcccctt ggggctggcc gagcaggttc cggcaggatg
     8221 gaacatcatg ccgcgcacgg aaaccctgag ccccaggcag cgcgtccggc tcgcactcaa
     8281 tcacgagatt cccgatcgcg tcccgatcga cctgggcggc tttcaaacgg gcattcacaa
     8341 ggacgcgtat gtggcactga tcgagcacct gggcatccac gattccatct cgatcatcga
     8401 cgccgtccag cagctcgcca gaccctgcga ggaggtgttg cagcggttcc acgtggacac
     8461 ccgctacctc tgtgcccacg ctcccgacgg cttcaaggga ggcatcatcc agaatcgccg
     8521 tgacggccgc ctgtggcatg atctccgcga cgagttcggc gtcatctggt ccatgcccga
     8581 cgaccagccg ctcttcatgg atatctctta ccacccgctc gcccgggcat cgaccgccga
     8641 tgtggcttcc tacccgtttc cccggggcaa cgatcccacc cgcttcaccg gcgtacgcga
     8701 gcaggccctg aagcttcgca acgagacgga ttacgccgtc tgcaccggta tcggcggcgt
     8761 ggtctacgag tattgctggt acatgcgcgg tctggagcag tggttcatcg acatgatcga
     8821 gaaccccgcg ttctgtgagg tcctgctcga ccggatgctc cagtattgga aggattttta
     8881 cactggattc cttggacagg tgggagactt ggtcgacgtg gtgatgatcg gcgacgacct
     8941 tgccggccag cgcggaccgc tcttcgatcc ggccttctac cggcgcgtcg tcaagccccg
     9001 gcagaaagcc ctcgttcagc acatcaagcg gctgacccag gccaaggtat ggtaccacac
     9061 ctgcggggcc tgctccctgt acatccccga gctgatcgac aacggcgtgg acgtggtcaa
     9121 cccggtgcag atcggcctgg aggacatgga cccaacctcg ctgaaggagc ggttcgggtc
     9181 gaggatcagt ttctggggcg ggggcatcga cgcccagcat gtcctgccgt tcgcctcccc
     9241 agcccaggtt cgcgaacacg tccgcgccaa catagccgcg ttcatgcccg gcggcggata
     9301 cgtcttcaac aacgttcata acatccagca cggcgtcccg cccgagaata tcgtcgccct
     9361 cttcgacgcc gcctacgaat tcggcttcta cgagtgacct cgcacccttg cccatggaaa
     9421 gctgcacggt gcgctccgcc atctgcaatc tcgaatcgcc agttaccccc attgactgat
     9481 gcgcgtcttt agtggccgtc gtcctgagtg cggttttgga ttccacggcc gcggccacag
     9541 ggttccgggc ttgcgttctc agaggcgccc gccaagcaga tcaaggggtt tccgttgctt
     9601 ctcgtcgggg ttggtcttga gggagacctc ggtgcttcgc tcttgggaca acctcggcgg
     9661 acaaagtccc gcatggcctc caaccgctcc agcgacaaac ccgccacccg ccctcgcatg
     9721 tggtggcacg ccttgccgtt ctcgcgaaag atctcccaaa acacaccgta cacgcaagga
     9781 tgcttacgcc gccgagccgc caaggtctcg ggggggcatg acccgcctcc gtgcagggct
     9841 cactacaaga tatacgataa agattcaaaa acataaccaa aaatgcacat ggccgcatct
     9901 gaaaggcagc acggcagcca actaccgcag tcacagatac ttacctggtt tcgcgggggg
     9961 gtaagttgcg gtaagatccg gtttccccga ggaaccctga aggcccaaac ccaacatgcc
    10021 ttgtggagta gccaacatga ccccgttcca cacccagact gaaaacgcgg caagctgcct
    10081 tggttcggac ctgcccagat catctagaag gcgttttctc gccggcgcgg cagcatgggc
    10141 aggcagcttg tatacctttg ccggttgttc ctcgcgaggg ggcagtcaac ctgccacagc
    10201 tcctgaagga gtgacccgcc gcctgcgact cggcctggac acctacagcc tgcaccagcg
    10261 cctgaccgcc aaggatcccc gtaaccggaa ggacctgtgg tgggtgctgg atcagcttga
    10321 gccctgtggg cttgagggcg ttcaaatcga ccccagtcac tttcccggag acgatgagaa
    10381 gaccctggaa cgactggcat cggtcgtcgg ccctcgcggt cactatgtcg agtttgggat
    10441 gggtggctgg gacccgactc gtctggagca tcgcgggaag ctgacggtgc ggttcggcgg
    10501 ccgggcggtt cggaccttct gcggcggaga gaccaccagc catgacaaaa tcgccgagta
    10561 catcaagtgg gctccgccgg ctctcaggca ggctgccgag gcggccgaca ggtatggctt
    10621 cgatatcgcg gtcgaaaacc acggagactt cacttcgact caactcaagg ccctgttgga
    10681 cgcggtggac cacccccgcg tgggagcctg tctggacacg ggcaacagcc tgtttcgcaa
    10741 ggaagacccc attcattgtg cgagagttct ggctccttat gcgaagtcga tgcatttgaa
    10801 ggattggacc atgagcttcc ggccggacgg gacacccgaa tggaaagaag cggtactggg
    10861 gacaggacag gttccggtca aagagatgct tcgcatcgcg atcgaagcaa accccaatct
    10921 gtacattgct ctcgaggctc ccgtccggcc aagcgaggat gagacggaaa ccgttgatcg
    10981 cgaatggcaa ttcttcaagc aaaacgctcg cgttgcacgg gagctgctgg ccgacttgtg
    11041 accggcaagg ccgccggcgc gcacaagcga ggaggcattc acgtgaacca caagatgacc
    11101 agacgccgtt tccttcgttc caacgttgcg acggcggtcg gcgcgggcct ggccggccgg
    11161 ctggtcaggg gagaaaactc gaatccgaca acccgaccga ccgacagcct gtcggtcggc
    11221 atcatcggat gtggcgaccg aggcaagtac ctgacctatg tctgccagat cgtgccgggc
    11281 atccgcgtgg cagcgatttg cgacgtcaac cgcgatcggc tggccaaggt ccatcagcag
    11341 cttgggggcc aggccgacac tcaccacgat tatcggagga ttctgagcaa taagagcatc
    11401 gacgcggtcg tcatcgcgac gaatgctcac tggcatgccc gtatcgcaat cgatgcctgc
    11461 gaagcaggca aagacatcta tctcgagaaa cccgtggcca catcgatcgc cgagggacag
    11521 gcgatcatca aggcggcccg ccgttacaac cgcctgatcc agatggggat gcaacagcac
    11581 agttgggagc actaccgcga agcggttgaa atcgtccggt caggcaggct ggggaccgtc
    11641 tcacacgtcc atatctggga cgtcgagaac gcatggcccg gtttcggaat gcccccggat
    11701 ggtcctgcgc cgcccgaact cgactgggag ttctggctcg gaccttcgcc gctggtcccg
    11761 tacaatccca atcgatacac ccgcgccgag tggttctttg actatgacgg aggatggcag
    11821 gttgcctggg gtgcccatca cttcgacatc gtgcactggg ccatgggcgt cacggctccg
    11881 gtggccgcca ccggctcggg cggtcatttc gcgttcaagg acaatcgcga atggccggac
    11941 acgttcaacg gcacctgcga atacccgtcg ggtcccgtcg ccaagaacgg gttccttgtg
    12001 acttatacca tgcggcacgg aagcggccgc ctgatcgagg gacgggccca tggcaaggcc
    12061 tttcacggaa ccgacggcgt tctgatcgtg gaccgcagcg gttacgaggt gctatcggaa
    12121 aaccgcgacg gcgcgaggat ggtcgcggaa gaaaaagtcg cctcttacac gcgatctcac
    12181 gatgtcgttc agaaccacgt gaaggctttt ctggaatgtg tgcgagcgcg caggtcgccg
    12241 gaagccgacg tcctggtcgg acaccgggcc accaacccgg gccatctgat gaacatcgcg
    12301 tggcgcgtgg gccgccgcgt gcgctgggag gggacgaccg agcaggtcgt tgaagactcc
    12361 gaggccgcca aatggctggt taagacctat cgccgaccat gggctctacg tgagtcgtga
    12421 cagaccgacg cctggaacaa agcgaacaga tgatgaccgc agatgtgacc tctcgtggcc
    12481 gatccgccgt tggcctcctg gtgaccggcc tgacggtctt gggctgggcc ggcgcgggca
    12541 aagccgagca accgagaaga acggaagtcg ccgttcgcgg cgatgccttc ctgatcaacg
    12601 gccggccgac ctacgaaggc agaatctggc aagggcataa gatcgaaggc ttgctgttga
    12661 acagccgtat ggtacagggc gttttcgacg acctcaaccc cgacaccaga gcccggtgga
    12721 cctatccgga cacgggtcgg tgggaccccg atcgcaacac gcgcgagttc atcgctgcca
    12781 tgccgcagtg gcaggcacac ggactgctgg ccttcactat caaccttcag ggcggatgcc
    12841 cccagggcta ctgcaaggaa cagccgtggc acaactcggc catcgagccg gacggatcgc
    12901 ttcgcaacga ttacatgaga cggctggagc gaatcctcga caaggccgac gaattgggta
    12961 tggtcgccat cctcgggctg ttctacttcg ggcaggacca gcggctggcc gatgaggcgg
    13021 cggtcgtgcg ggcatccgat aaggtggtgg actggctggc cgaccgccgc taccgaaacg
    13081 tgctgatcga aatcaacaac gaatgcaacg tcaattatga ccatgccatt ctcaggcccg
    13141 accgcgttca cgaattgatc gagcgggtca ggcaacgctc ggaccccgtc cgccggttgc
    13201 tggtcagcgc cagctatggc ggcggcgccg tacccaacca aaacgtggtc cacgcctcgg
    13261 atttcctgct gctccatgga aacggggtgg gcgatccgaa ggtgatcgcc tggatggtgc
    13321 gtgaaaccag aaaggtgccg ggctatcggc cgatgccgat cctcttcaat gaggacgatc
    13381 atttcgactt cgataaaccg gtcaacaact tcgtggcggc cgtcgcagag tactgttcgt
    13441 ggggtttctt cgaccccggc cgcggcaact accgcgatgg ttaccagtgt ccgccggtca
    13501 actggggcat caacactgac cgaaagcggg cgttttttga aaagctcagc gaaatcaccg
    13561 gccggcaccc cctaacatcg cgtcccgccc gttgactcgg ggccgccgat cggcggtttc
    13621 ggccatttga acccttcgcc cgtgaatctt acaattgaca ggtgatgact tctaatcatc
    13681 gcaagcagca tctcctgggg acaccgctga ccggctttgc gacgttctgc gcggagcaga
    13741 aatggccggt ctacagggct cggcaggtct accagtgggt tttcgatcgc ggcgtgaccg
    13801 atttcgaggc catgaccaac ctcgccaaga ccgaacgggc caaactggcc gcgagctgga
    13861 ccatactgac cggcaaggaa ttgcgacgcc aggaatcaga ggacgggaca acgaaacttc
    13921 tgctggagtg gacggacggg gcgaccagcg aatacgtgtt catccccgac gaaacacgcc
    13981 gaacggcctg catctcaagc cagatcggct gcccggtcgg atgcacgttc tgtgccagcg
    14041 ggatgggcgg gctccagcga caactgaccg ccggcgagat cgtcgaacag gctgcgcgga
    14101 ttcgggctct ctgccggcag ccccccgaag atcgtcgttt cggcgaagac acgcggctca
    14161 gcaacatcgt gatcatgggc atcggcgagc cgctggcaaa ctacgagaac gtcctccagg
    14221 ccgtccgcat gatcaacgcc gattggggca tgaatatcgg ggcacgcaag atcaccctca
    14281 gcaccgtcgg cctgcccaag cagatacggc ggctcgcgga ggaagacctg cagatcaacc
    14341 tggcgatctc gctgcacgct cccaccgact ccctccggcg caggctcatt ccctgggcca
    14401 atcgggtctc ggtcgacgaa ctcgtgtccg cggcgaactt ctatttcgag aaaaccggac
    14461 gtgaggtcac gatcgagtat ctgctgctcg gcgacgtcaa cgaccggccc cgacacgccg
    14521 ccgacctggc aagcctgtgc cggcgaatgc gatgcaatgt caacctgatc cgatataacc
    14581 ccgtcgcggg attggggttt gaacgaccga cctcggcggc agcccatcat ttcgtcgagc
    14641 aactgcgggc acgcggcatc aatgcgcacg tccgcaagag ccgggggtgc gacatcgacg
    14701 cggcctgcgg gcagcttcgg cgcacgcacc gcggtcccgg gtcgcccggc gctgttgaca
    14761 aacccgcctc gcccggttca aacatgacgg cccccgacgg ggcaattgag tgagagtctt
    14821 tcacaaagcg aggtcgcgag caacgggccg ctcctccgca gacgacggca tgttgacccc
    14881 acgcttgggg ctctgtcggg gcgccccgaa aacctgagcc aaggagaaaa cagcgatgga
    14941 acccctccag atcggcgtgt gcagttggtc cctcaagatg ccggatatcg gcgacgcact
    15001 ggtggccgtc aagcatgaac tcggcctgaa tctcgtgcaa atcggctttt gggacgaggg
    15061 cttcaaacag acggaccgga tcatcaagct catcgaacga accggccttg aggtgagtgc
    15121 cacttgcatc ggcttcgagg gcgaggacta ctcgagcatc cagcggatcg ccgagacggg
    15181 cggcttcaag cccgacaacg agtggccgcg gcgcctcgcc aagaccaagg cgtttgccga
    15241 tttcacggcg gccctcaacg tgaaaatgct ggcgtgccac atcggcttcg tcccgcacga
    15301 caagagcgaa tccgtgtacg cggtcatggt ggaccgcatc aagcaggtct gtgacgtgct
    15361 ggcccaccgc gatctgacac tcgtcatgga aaccggccag gagcgggccg agaaccttct
    15421 ggagttcatc gacgccgtcg aacgggacaa catccggatc aacttcgacc cggccaacat
    15481 gatcctctac ggcgtcggtg acccgcttca ggccgtcgac attctcaagg acaaaatcgt
    15541 tcattgccac atgaaagacg ccgtctggtc ccgggagccg acgaagatct ggggcgaaga
    15601 agtcgtcctg ggcaccggcc aggccaacat ccccaaactc atcgccaagc tccgctccta
    15661 cggctacacc gggccgttgg tcatcgaacg cgaggccggc aaccagcgaa ttgcagacat
    15721 caagacggcc atcggcttgc tggaaaaagt agtgggctaa ttcaagctct tggtcattcc
    15781 caggcaccag cctactcatt cgccttgacc gacgcagcgg tcattgtgca gaatcatatt
    15841 cgtggtgagg acggcgggca tgcagcgtgg tgacgatgcc tgcgtcgatc gagttctcgg
    15901 gcaagccgcg agcaggtgac gcttgagcaa tccagaccgc catcagtcga ccccggccga
    15961 cgagaacgta caactgcggg agcgccttca ccggcttgag catcccgggg aggaaaagga
    16021 ggtcttcgtc catctggcca ggagattggc cggcgcgacc tcggccgatg cggtcatggc
    16081 tgccgcgcag gaggccagcg agcggcttct cgcctgggac ttcttctatc tggcgcaccg
    16141 ccttccagag tcgaatcagt tccgcatcgc cggagtcatc gacaccattg aaggacgccg
    16201 gcataccttc cccgaacagc gggatgccga ggccacattc agtcctccgg tgcgcgaggt
    16261 gctcgccggc aaacgactgc tggtcaatcg ccgggagggc cggacaacgc ccgtgatgcg
    16321 accgcttggc tcgggtcggc cctcagccag tctgatgttc gtccccgtgc agtgcgaagg
    16381 ccaggtgatc ggccttctgt ccgcgcaatc ctacgtgccg aaccagtatg gcgaatccga
    16441 cctgcagatt cttcaggacg tcgcggacgc cgtcgctccg gcaatggagc gcgtccgcgc
    16501 cgaggaatcg ctccgcagcc gggagcaact cctgcgcctg gagcgggatc tggccgtttc
    16561 gttgggcgcc gcacgggacg tgcatgaggc actggaccga ctcctggaaa cgagcctcca
    16621 gattgaaggc attgattgcg gcggggcgta tcttgtcgat cccgccacgc acgatcttat
    16681 cctcgcgcgc tacaggaatc tgtccgcggg cttcgctgac ggcgtcaaac atctggcccg
    16741 cgacagttgg caggccgcgt tggtccttaa ggccaaaacc gtctacatgt cgaccgccga
    16801 actgcactcg atcgatgcgg cttgccgcga cgagggcctg atgtccgtgg ccgttgcacc
    16861 cgtcgcacaa tcaggcaacc tgatcgccgt gctgaacctg gccagccgcc gcctgaacga
    16921 aatcccgcct cacgcacgtc acgccatcga ggcggtcgcc gcccaaatgg gtaccgccct
    16981 gattcgcatc cgcgcggaac aggacctccg ggaggcgaga gaccaactcg agcaacgtgt
    17041 ggagcagcgc acggccgagc tgcgcgaagc caacgcccgc ttggaagccg aaatcatcga
    17101 acaccagaaa agcgagcagg ccttgctgga aaaagaggcg caactgcgca gcttgaacga
    17161 gaacctcgcc gagggaatgg tctaccagat caacagcggt caggatggtc aaaagcgaca
    17221 gttcacgtac ctgagccctg ccgtggagca tcttcacggc ctgagggtgg aagatgtcct
    17281 ggcaaactcg acgctcatct acgaccaggt gatcgaggag gaccggtcct tgctggccga
    17341 gcaggaaacg cgggcctttg cgaccagaac cacgcttgac gtggacgtcc gggtccggct
    17401 tccatcggga gagatccgct ggcgccgttt catttcttca cctcgggtcg ttccggacgg
    17461 aacacttatg tgggagggca ttgagctgga tatcacccgc cgaaagaaag cagaggaggc
    17521 tctgctgcgc aaggaacggg aactccgatt attgagtgcc gaggcaaatc gccggctcga
    17581 agaagagaga gcccgcgttt cccgcgagct ccacgacgaa ctcggtcaga tgctcaccgc
    17641 cctcaacctc aacctgaggt ggctgggccg tcgaatcggc gacgtcggag acgacgtcgt
    17701 cgagcgaatc gacgaatcga ttcagtacgt gagccggatg atcaccagcg tacggacgct
    17761 gtcccgcagc ctccgaccgg ccgcactcgg ccacgagggg ctggtcgagg ccgtccgatc
    17821 gcacatggct gaattcgaac agtacgccgg catccgctgc agagtcgagg tggtaccgcc
    17881 gaacctcgcg gtgagcgagc ctctcgcaac caccgccttt cggattgtcc aggaggccct
    17941 aaccaacgtg gcccgccatt ccaaggcaac cttctgccgg gtgaacatca gggcggtcga
    18001 cgacctgctg gtgctggaga ttcgtgacaa cggcgtcggc ctgaaagaac aggagccgac
    18061 cgacgcgatt tccttgggaa taccgggcat gagggagcgg gcggaaacga tcggtggaag
    18121 cctcgtggtc gcgaacaacc ccgagggagg cgtttgcgtc accgcgcgcc tgccgcgcca
    18181 acgccgcgat cattcgcccc tcggttcata gtccgaccgc aaggccgcac gccgggacta
    18241 tttgagactc agccgaaatt cggcgtagac catccgcggc acttccgcat tgttgagaaa
    18301 ggtcgtcccg ccctcgggat ggtccgggtc cagaagattg cgaacaccca ccgccacggc
    18361 ggcctgtttc ttccagaact cgtactcggc cctcaggtcg agccgcaagt aaggcggaat
    18421 ctgcatcggc agaagagcca tggtcccgtt gggtgctttc accgtatcga cataatgcag
    18481 ataagccgat agatgcaggt ctttcaccgg atcgtactgt gcaccgatca tgaacttgtg
    18541 ttcgggcggg gtgatgaaat ccttgtcggt gtacggcacg tgcccgaacc aatccatcca
    18601 ttgataggta tagtgggcca gcaaagtcaa ctgtttcgtc gccgcgtatt tcgcctcgaa
    18661 ttcgaacccg tacatcgccg cggaggcccg gttgtcgtac ttcgcctgaa gcaggcccgg
    18721 cgggccgaat tccatggtcg tggcggtgat cacgtcgtcc atgtcgtgcc agaacaggtt
    18781 gaaattcgtg gtgagtcggt ccgaaaggaa cttcttccgg tatcctaact catacgcaat
    18841 caccgtctgg gggtccgtct cggggtcggc ggtctggtgt atcaggccgc tgtacagcac
    18901 gaaatcggca aagcggcctc ccaccggcgg catctggaac gcccgagaca ccgctccgta
    18961 ggccagagag ccgtcggaaa acttgtggga cagggcgatc cgcccgctcg gctgaaagcc
    19021 cccataaaac tcgtaatcga tccgggcgcc cagctccaga gcccatcgcg gagcaaacct
    19081 ccactcgtcc tgcacgtaaa gaccgatcgt cgcgctgctg acgtagtccc gggtcatcaa
    19141 gtgaggatcc gagttggtgc cgtcaatcag gtcggtccgg ctgtccagac cccacgtcag
    19201 cgtgtgattg tcgcccagct tgaagttgtg attgaactcc agagcaatct gctggtagcg
    19261 atagtcgatc gtcttggcgc cgggctcgaa atggtagtcg ttgacgtaag cattgatgcc
    19321 gaattggttg tcacgagcga cccgatgatc ccacttgccc agcaggaagg cggtctgcga
    19381 cctgggactg agtttgtccg gagggaagaa gctcggagaa gtcgggttgc ccccgccggc
    19441 tacgctgtta ccgcccgaca gagtgaaaac atccttcggc gtggcgtcat agaccatgtg
    19501 gacgccggtc cgtccgccca gaaaatcatc atcgagttgt tgcaggatcg aaccgccctt
    19561 gcggaagccg tcgctgctct cgtattcgcc tgacacccgc agtttgagct tctcatcggc
    19621 atagccgtat cccaggtgct ccttgtgggc ccctcgtgat cccccactga cggtactggt
    19681 cagacccacc tgatcggcag ggtccttggt cacgatattg atgacgccgt tcagcgcgtt
    19741 ggcaccccac gtgacgcccg ccggaccccg gagcacttcg atccgctcga tatcttcaag
    19801 ctggatcggc cagaaatacc agaacgtcgc tcccaggatg ggatcaaaca tctggcggcc
    19861 gtccatcaac accagaacct gatcggccgt cagtccgtgg gcgcctcttg tcgacacgcc
    19921 ggagtaaccc cagctcaggt ccgcaacgtc cactccgggc aacaatcgca gcgcgtcggg
    19981 gaccgaacgc gcccccgatc gacggatgtc gtcggccgtc accacgctga cggcgtaagg
    20041 caagtccatg atccgcttcg ggcttctggt tgcggtctgt accacgtcta cttcgggtat
    20101 gtccagattg aacacctcca tgtcgttcaa gccgtcctgc ggcaggctga accgatcgag
    20161 cgcttcggcc tgccccacgg gatcctcggc cggtgcactg gccggccgcg attgacccag
    20221 aactgggacg ttcgcaagaa aagtcaaagc gatcgcccat gccgccggcc acggccgggg
    20281 acgaaaccgc cttctccaga cgtccctcgc cctcggctcc accgtttgca cggctcgagg
    20341 catcatgtcg atcaatctcg ttgtacgccg cagtatcctc acctagtcgt tccttccgta
    20401 ctgttcagga atcttggcca atatgtcgtc ggccagcgaa ataccgatca tctctgtgct
    20461 tcgatggttg atcgagcaaa tgaccttggc cggatacacc accccgatcg atgccggttt
    20521 ggtgccgccc gcgatcttgt cggccagatc gaccgtctgg cgcccgatag actcgtcatc
    20581 ggcctgccag ccggcaaacg ctcccgcctt gacgatcttg cttgagaaag cccaaaccgg
    20641 cttgcgagca cgaatgcccc agagaagaag gcgctgcgcg gtggccgtgt tatacacgtc
    20701 ggcatccggc accatgatga cgccgtcgca acgattgctg tccagaagac gcgcggcttt
    20761 cagaaaatcg ttcttgctcg tttcgatcgg cgtaatggtc attccgcgat cttgccccgc
    20821 tttgcgcagc gcctcgaccg tgcgacgcgt tcggctgctg aacgggacgc cgatgttctt
    20881 gacaccctcg tccatttgcg tcacccacgc gatctgctca accggcgaaa tatccgtggt
    20941 gacccccgcc acgcgggccc tgtgtcggct gtcctccgcc agaaaagatg cgtccagagc
    21001 gttgggcacc atgcaaaaca cgaccgccgc atcgggaacc gattccagcg ccagaatcgt
    21061 ggcctccgtc ccgaccgcga tcaccaccgc cggccgagtc tgaacaaggc ggctccggac
    21121 gtcctggaga gaaacacccg aagacgccgg tcgcgacgcc ggcgcgggcg aagctgaagc
    21181 cttgtccgaa tcgacggagt cttccggctg aggttgctca gggggtgcgg gcaactccac
    21241 catgacgtaa gaccacttct tctgtttcgc ggcggtttcg atgctgtcga ttgtcttgcg
    21301 ataaatcgct tgtggggcag caagaatcgt gattctggct gactcggcgc cgtgcgtcgc
    21361 ctgcgagttg tgacctgcca ccagggcaag caggaaaaga atgaccgtcg tcattcgcct
    21421 gcctggcgac cgcaccccgg cactcgacac tggcatcctc ataggttgaa cgtacgtgag
    21481 cagcgcggcc cgctcaccaa ggccgtcttg gtcttgcccg tcgcaaccag tgcccattct
    21541 gcgcgcacgt ttcgggatca cagcgcgttt gggttgccgc ccgcaaaaag gcggtccgac
    21601 ctggatgaag gtcgcggcga cctggagcct atcaccggag tccgtgtggg accgccttcc
    21661 agccggtcga cgttcggggt ttccggatag gtctttaatc gcaccgtctg tacgccgccg
    21721 tgacagacac cgatcacgtg gagaatcgcc tgtccacgag ggtggaccat actaatggct
    21781 tttatcggtc atcgggcgcg ctatctggcg attttgattg ggcccacgcc cgctcagcgc
    21841 ggagaacaag tcagggcgga ttctcgtcag cccggcttcc tattgactgg caatggctgg
    21901 tatgcctcgc accgatgtcc tgtgcggtcc gtgcatggct tgacagtatt gataagcccg
    21961 tcatttcgcg gcttgccgcg tcgccacgct gcccggttcg gcgccgatga cgccgcccgg
    22021 ccatcgaaca agcgtcagcg ggtcatgggc aggcaacaca atgtcggcca gtgtccggat
    22081 cttggccatg gcttcctggc agactcgcgg atcgggactc cgggccgggc ggtctcgttc
    22141 gatgttctcg tacaggtaga caacatcgcc cgtgagcatc actggcccga cgtgcgtctg
    22201 aaccagcact ccctggctgc cgggcgtgtg gcaacccagc gggaccgttc ggatgcccgg
    22261 aaggatctcc tggtcctccg ccagaatgag cttttcagga tgcgagcgga tcgcagcgaa
    22321 aacttcggga ttcaagtgac cgcggtcctc ggggttgtcg gcgtcaccgc gagtctgttc
    22381 atactctcgc cggttcacca ccaaccgggc gttggggaaa gcagggtaat agtcgtaatg
    22441 atccgcgtgc aggtgtgtca cgatgacgtg actgacgtca gatggatcga tgccgacccg
    22501 cttcagagca atgagcgtgt cttcctccgg caattgcctg acgccgccgg gaatgtactt
    22561 ggccgtggcc ctgttgaaat cgccgacatg cttgggattc gggccagtct caacaagaat
    22621 cgggtgcggg ccgccgagaa cggcccaaac gtacaggtta tacatgcgcg tgtcctggcc
    22681 ctcgctgtct tggaagctga tatactcgcg gacctcacat tggccgacct tgatggcccg
    22741 gatggtatat cgcggcaaac ccggcgcgac cggaggccag cgacgagggg tcgtcgggtc
    22801 atggctgggt aggacgatgt ccgctctttc gcggatcttc ttcatgccgg ccctgcactg
    22861 atccaggtcc atccgcacgc cgacaggaat gtccttctcg atgttggcaa gcagcgacac
    22921 cgtatcgccg gtgagcaccg tacgcccgtg cgcggtgttg acctgatagg ccgtcgagcc
    22981 cagcgtatgt ccgccgacaa agaacgactc aatgcccggc aagacctcct cgtcctcgac
    23041 gagcaccaga cgcttgttcc actcggggtt gtcccgaagc atgaacatga tgcggccgtg
    23101 gccccaactg cccttgcagt cattggccgt ggccagttgc cattcttgct ttccgatgta
    23161 gatcctggcg ttggtgaaag catccagccc gtccacgtgg tcgaagtgca ggtgagtgat
    23221 gaacacgtgg ccgatctccg cgggcttcag gccgaatcgg gcaagttgcg cggccacggt
    23281 ttccgtcggg tcctgagtga tcggcttctt caacaccgcg gccgctcctc ggttcatctc
    23341 cgcgacatcg ctcagccccg cgtcgacgag catcggcttg tcgcccccga gaatcagcca
    23401 gacatagaga acgtattcat aactctgcgc cggatcgccc ccctcaaacg cgtcattacc
    23461 ggcgatctcg catcgaccgt tgcgaagcgg gtagatgcga tacttgggga tatctttacc
    23521 gaatctcgta ccgatggccg ccggcttgga cgcccgggac tgctcggcgc aaacccaaca
    23581 tggcaggacg acaacagcaa ccgcagccgc gacgagcacc acgccccacc ggcaccgccc
    23641 ggcttgcctt ctgttccagt acgccataat tctgaccata gcgcgagcgc ctctgccgcc
    23701 gctcaccagg aaacacgacg cccaacagtg ccgctgcgat cagacaatat agtctcttcc
    23761 gtcgcgactg gtgcgcattt tgcgcgcatg tttctggatc gcggtaccct tgggttgcgg
    23821 cccacaaaaa ggcggtccgc cttaggcgat accggcctgc ttggcgattc ggctgctatc
    23881 cgggtaccgc cgccaccctg cgcgtggcac cctctggacc ggcaatcggc ccacgaaacg
    23941 ccctcgccca ggatgaacgt ggcccttgct ctccagcccc acgcccaacc cgcctccaaa
    24001 ccgtaatctg atggcacgaa cagatcaaaa tcaagtgatc tggtttttca tttatggttg
    24061 atggtcatta ccctaatcaa catattattg cctcagatga tcctaatgcg ccaatacttt
    24121 accgaagctg aaagccgcat gccgttcgac cagcgcgagg acgaattctc ggcagtttct
    24181 gtcttgccgc gctatggggc acccgttatg atacaggctg tcacttagaa ccttacagcc
    24241 agacatctgt gcgccttgcg cgcatgtttc tggatcgcag agcgcatggg ttgcggcccg
    24301 caaaaaggcg gtccgcctta acactccaag gaggcgaaag gctgtagagg gcaaacgacc
    24361 aagccaattc gtccgagggt ttcaagtatc ggcggtcttt agtctttcaa agaggaggag
    24421 tcgaagatga agaactttct aactattgct ggtggattgt tggccgtcct ggcgttcggg
    24481 atgccggcca cggccgcagt cgtctggtcc gacggtgtga acttgcctta cagcagtgcg
    24541 accgtcgaca tcgagggcga gttcgataac gaggccgctt gggcgggtgg cgacttcaag
    24601 gttctcaatg gtaccgcctc acaggtgccc gctggctgga agttcgccca acagagctgg
    24661 aacaactggg ggaactgggc tgtcagttct tccacgaatc tcaccgaggg caatgccgcc
    24721 ctgaagatga cgcccggcgg tggagattgg aatgacatct tcgccggcac cttcatcacc
    24781 ggcctgacag tgggccaagc ttacaccctg acgttcgatt tccaggctga tgtcgggttc
    24841 gacaaaggaa atcccggact gaaaacggga gacgaccacc agcccagtgc ttccttcaat
    24901 gtcatgaacg tccccgataa tccgatcggt tggggagcca ccgaagatta ccccggccac
    24961 ctgaccggct cgacatggat cggtctgggg aactgggacg gcaattacca tcagcagtca
    25021 cgcgacttcg tggcaacggc tgatagcatg gccttcgtga tcaagatccg gagcatgaat
    25081 tctccgggtt cggtccggat cgataacctg gtcgtcacgc ccgaaccggc ggcgatgatc
    25141 ttgctggccc tcggcggcct ggcatgcctg tatcgccgac ggtaaggttg cccgaccggg
    25201 ctgacggaat tcaacagcac ctgagagcca tacgacggtg gtcgtgtggc tcttcttttt
    25261 aacgactgtg gtcaaaaacc ttcggatgct catccgatgc caccctcgcc caaaccattt
    25321 cggacttgtc ccggaccggt gagctccccg aggcagtgag acgattcggg cagggacgcc
    25381 attggcggca tgcctccggt cttttctacc gcccgagcct gcacgggcgg agaacccgcc
    25441 cgttccagcc acaacatacc cctgccggcc gcgccgcaga cccgacttga agccccctgt
    25501 tgagacacgg tcggccattt ccacgtcccc ctgccggtgg taagctgtca tcctcaaagt
    25561 cgcgccggat caccggtcga tccgggcatc gatcgagatt ctcaaaggga aagggttggt
    25621 tatgactcaa cgagcaaggg tgagtcggcg gacgctgctg aaaagggcgg ccggggcact
    25681 ggcagtgccc tatttcgtgc ccgcttgggc attgggacgc ggaggatggc tgcctcccag
    25741 cgaacgcatc accgtcggct tcatcgggat cggaaacatg ggcggggggc actttgacgg
    25801 cttcctggaa gacaagcagc tccaagtcct ggccgcctgc gacgtccgtc gcgacgtgcg
    25861 ggaaaggcga aaagaacagg ccgacaagaa gtacgcggcc gatcgccagg ccggcgacta
    25921 caaaggctgc gcggcttaca acaatttcga agacctgctc gcccgtgacg acatcgacgc
    25981 cgtcctgatc gccgttcccg atcattggca cgcgatcatc gcctgcgcgg ccgcccgggc
    26041 aggaaaggac atctactgcg agaagccgct gaccttgacc atcaaggagg catgggacct
    26101 cgtggatacc gttcggcgct atggccgcgt attccagacg ggcagccagc agcgcagcga
    26161 ccgcgaattc cgtctggcct gcgaactggt ccgcagcggc cggatcggca aactgcagac
    26221 ggtcaccgtc aacatcggcg agccttcaca agaagcctat ctgcccgagc aacctgtgcc
    26281 cgagggcttc gattgggatc gctggttggg accggccccc tggcaaccat acaacgccga
    26341 acgatgcagc ggcaactacg gcggcgggtg gcgaaagatc cgagactact ccggcggcat
    26401 gaccaccgac tggggcgctc accactatga catcgcccaa tggggcatgg gcatggacgg
    26461 cagcgggccc gtcgagatca t
//
LOCUS       DAOTPW010000048        26192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1936_length_26192_cov_6.133795, whole genome shotgun sequence.
ACCESSION   DAOTPW010000048 DAOTPW010000000
VERSION     DAOTPW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26192
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1936_length_26192_cov_6.133795"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1398)
                     /locus_tag="P5316_06315"
     CDS             complement(217..1398)
                     /locus_tag="P5316_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR84609.1"
                     /translation="MRRVTCCLIILGGIVGCGPPEALREMHAAFNQGQWAAVTEKFAD
                     LGRPRDLNGVSYARVGIAATEVYVKSKSPAYLDLAIDCYQLAADRDRSLADGHHFARG
                     ELLLYANRKDEAIAAYLDALAVNARHVGAAWRAAVLLTEAKRAADASRAARGLLAVRR
                     PVNQQLLQGLSALASAEASLGRHDAAIKVLRHAASIGPSTWGPDILYALACCYQAQNE
                     NGPARMAMRRALAALPEGDSRRPRFDKYLEDLERASRTATREPAPEPTIDTGPRVAPP
                     PQEPADSAPEQPAPAPPPLRGHSPEARAVELAAKASVLAAENKVEEAISLLEEAVGLA
                     PRLPAPPAQLANLYVKQGRYAKAVEVLETAIGHNPDNVILKQMLDKLRSALAAESRPG
                     R"
     gene            complement(1428..9548)
                     /locus_tag="P5316_06320"
     CDS             complement(1428..9548)
                     /locus_tag="P5316_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84610.1"
                     /translation="MSIAQRRHAALLPFLALFEQRRCPSCPILFGTLVWLAGWIAVVP
                     AADPTTPGETPPPAAAASPDPGAAEPGREGVPDPVLQLGSVSVPLSARVSELPRETTT
                     DNQPRWKTLDWAIRAFTDFVNSEWNYGTQADQDRKILDQLDREVDAETTRLREEYKQK
                     LIDEWRAVTKDTTRSPPDELLKQAEKATLLQIPGNIRTADRFHKLLVTIYEDGGQKRV
                     KYLVDRGAISQKLGELLNRVRFEAYTAAMYDVAKEYHGGDVSVFWFSFPKLISSDIDH
                     TVVGVKFPDGTIIPAAKVIDRFNARFEVHLVPPDVADVMAHDARNRPPDLFSGMIDDG
                     GTLEIRLRPEADAAYYEDAGRATKNLQHTKDAYWLFAPFGTQVDRRSLTGTAEENYFR
                     ITAEDVGTPDNPRFVYREERVTAREVIYKNIPPLLSQKVGFDTMVGNWLLATDHAAGK
                     DPTAASTLTEQAKYLLRGVTEGMLTSALENGKPKYDPYEPPDWGKIPNAERRRMIDEI
                     AGRQFPDEASRAHFQALIDTADALRANKGDAAFAPMIEWYRQHGPEAIRKLPSDEAIL
                     VAAKENFGDRARAALMQGALAVAEDRVRAIISPDIPLSYQDRILKLATEGEGDAKVRA
                     NQIVEILGDKTARATVTRRLKHVWANQFHHALNALDDANFDRAAQRFDEVIKNLDAGR
                     EDRALMKSIMDNLRTRRTRLRAIRSDLEARLTRIEDPPPTRIGSADDNAESVTNYVRG
                     QQAELKRLIQEFGFDLPDAQLRQRVQEALDAKPVYRPVSERVPTFSERISKVFAPDGE
                     FSRLGRAIDTTAGLLGEATRYANSEVATHFGWDVDAETASMYRKAGLDPSGLPKQFQF
                     STLFKSQVNLGTMGSALTVYEEYKKDAAADPEAAWARAKARMLVEYAYAIPVYGDAVQ
                     LIDQAMAGDWYSAGSNIGMFALTRGSQPFTRWAMTHAGYAEAQAAETAAAVGNVVGSL
                     AQGWAAYGLGRRIGETYVGPGIYKNAALEFYEGVDPTKGPDSWGATLAQWAARQLNAA
                     ADGLTSLEGWFFPKAAPDLEKTMGWLLTGYPNKARYRLKQLDRILALDLRQQTDKPAA
                     QAYVKVADELIQTYRSRVAKALQEYNSSRRYSELLHDDPWVKLTQETIDNVIAKFPTL
                     PDLPMMLDGTGIVGVKELDDKLWKAGVEPRSWELYVRMLRPEMVGINRFPDDPPGGAD
                     APPLDRYRKELLDKATDAWKPFLEAERYKDNQTTPEYEAFMSADLAVREYRLKELDRI
                     LRAKPGDVPALSASMLNAARRARDTIAAQVERIRETDRRGYYADDFRQSAAEMPKNAL
                     PVAHDVWRALSEDVRRGVLANRRILDELGVSMAEYIFIAEWLRPEYARFDELPDTPPP
                     GTSMDAWRQKLIGEARSIWDKFIWSELDRRGMRPPVDPRYVTPVIPAEKISVLKWVPG
                     ADAKEKRENVYATFFPMIRDRWIQEVGWAKLLGPGGGRGQSKLTRTGLTEFVVNSLGG
                     PPPERKALPLELAVHHEVARDFFFHYVENFATGGYHDIYLNRETPLPGRITFENMSKC
                     ANPWLWDVETRMWFHQSGKYYWALMESKDAKDLLVSMLYNDYVTGMTKRLAEVGGLQQ
                     ADLEDDAKIGHVCRRMYDKVLRNTVPSSDVTTLSIVQQLYPESRANPPPDSDDWIKYQ
                     AMPITGKAVKIRGKPFLIVPRLDQEVTLNLHAEVSVPRYAAVRPFEYEWAIQGASNAN
                     IGDREGVGLKLSDLTPGQKVKVSVRVYDASEARKAIGSDEIEVTVVGGAALVDKVAVY
                     HTDAPDAPLGEWEAELVATRIAAGHGYELEIAGQASDEYTGIRRIGVGLIPRKAVIDM
                     LGESSCWQRHLISSDTLLAEPTFSSSPAPAPSADNGASSAGTTPGSAPPAGGATASGE
                     PNDERLWRSLVDVATWKTIDSPDPTQPAKEKLRCHVSQEIETYVPFVVAESKDEGIRY
                     FEVARGTIEVVSAPMPEARFATAVLERELDGQKSVVNLLNGTSLIRDTHIVCQGKFGT
                     ARFTVLASTGGPKPEVLEVGYIKEEEQGQYKASSFRSITAEAKEVSAGTGEYSCSGEW
                     NASSAALGTYLLYARVKNPAPQADFFKLERLSDETSVWDLIQVEPSPDRDEDTSTAAS
                     RPGGAASQSASQPGSQPGDNGSDGTKPPIDPGLLDGLAEQAEVQAVEFKSTFMLQRIR
                     RDGDVRMPETFFARAMAVKLMPVVKVDNLYPGMKVHIETGYIEKGKIRRLFQQILPIA
                     GDKHEVSSLVLDLSKRGELAPRWVGKQIFRGDHVAFLHVQVVSRTGRYRTEVAKNEIA
                     FQVQEEGLGAASFKPMTFYDVRENNRMLAGQKFWDDANLRVDSPLEVSEVYGPELISV
                     THRFILASPSGKTLRWQINDRITVPGGGPHNTVKMLSLKPPIERGTWVVRHEVVDVKD
                     ADGIDVLKPGSPVFQSEGRCQVVEPKVLFKGLEDCYQIQHPPGNVKGAPVYEFLKETD
                     RPTARMALHVTDVPVDCNLRGSLTWVSPDTGRTILRLPAVVNMRENQGRIYFASYAFK
                     SDDYDGLYTAEVILEFYRAGRQPRAKLDLKPLRFRFKGRKRSGSTLEDITVASRRVDY
                     RIWDNGGLEDDVINVYVDGARVISGWKMPIAGKDFHLMIPADRDEVIFTIESVSSEEG
                     PNTAAITFKGAIEISRQTQSWWIDKPGQKASCVITYKPPATQPGQPRGGTSP"
     gene            9913..11646
                     /locus_tag="P5316_06325"
     CDS             9913..11646
                     /locus_tag="P5316_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84611.1"
                     /translation="MTCDTILGSKPLVLRQTGADGQTRQSPHDLQYGGAKALPAQPAA
                     VAHWPLTADVRDATGRGHEGLAHNITWNADGSAEFNGRDGFIEIPNHPDLAPGTGPFT
                     LTAWVYTETDLDDTLGDIAGKFDPSARKGFTLSIMNYPGVTSGQPNWRNLAFGIDDAK
                     ISQQWTDCGRPGNCKMLWTFCVWRDDLYAATFELEADQAGHVYRYTGGTNWEDCGSPD
                     AANAVTALAVFEDRLYAGTARYDSAGSSLPSSPNKNPGGKVYRYDGPGKWTYCGQLEN
                     PHTGKADTVHSLAVYCGKLYASSMYAAGKGLYEYQGGSGWKYVGHPGCRVEVLGVYNG
                     DLYTTSYDAPGRVARWNPTRGWQDLGAPPDTSQTYGFMPYEGRLMLATWPRATVYCLD
                     EADQWACQGRLDEESETMAMVVYNGKLYAGSLPLAKVYRYDGGRTWTETGRLDLTADV
                     RYRRVWSMAVFKGKLFAGTMPSGHVWCFEAGRNATCDHELPPGWHHVAAVRDENRLRL
                     HVDGSLVSRSEPFRLDDFDLSNNSPLRIGLGSHDYFRGRMADVLFYRAALSDDMIAAL
                     ARRRPRPTTRP"
     gene            complement(11690..13027)
                     /locus_tag="P5316_06330"
     CDS             complement(11690..13027)
                     /locus_tag="P5316_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84612.1"
                     /translation="MRSKVRICVVIAGTPADRSWGSSETEIAAVRKRLAEAEANLGNV
                     EFVIGQASTVEETTQLLAKSGPDAPVLAVSADIFGLSRGVMPTVFKEGRPVAVFHVPV
                     TGGHDWCLVNPWREEGHRITLFNSSDYDDLERAAALLRVIPLLRQSRVLASPPFKGTP
                     ASHDPEQVKSRLGVEIVAIADGRYDEVMAGVDAAATEKEAQRWLDEAKGMVEPTREDV
                     LKAARASLTLDQLIAESRADGLCVGTCMGWLPRGFPCLGFSRLNDRGIPAACDGDMDC
                     LLTMLIFRYALNRAGFLGNAGGVDTARNVFHLSHCSAPLRMEGPESPAAPYLIRRHAE
                     VRGGAVPEVHYRIGQEVTFTKLVHLDTLLLFTGKIVEVPEVPKGTERGCRTELVAEVK
                     DADKLLANWGGGALGASVKDYYASLHRVAYYGNHTRTIRHLAHLMGLKVVEEV"
     gene            complement(13500..14420)
                     /locus_tag="P5316_06335"
     CDS             complement(13500..14420)
                     /locus_tag="P5316_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR84613.1"
                     /translation="MIRRGENMCSRSRNAFTLIEMLVVAAILALLIAILLPSLNRARQ
                     VAKITVCKTNLDTMYKGHAFYAIDHKQHFPDPDWWLWDGIGGEMVNWFPNLYKGGSRP
                     ADSAQWVRFGHIFKYVKNAQAYFCPDDTLTRRAASIGSGQTVGGAFRGNKPIHSYVRL
                     IHPHQFYAAHIGSIADKIDNGSLPAMYRSDFIDPDRLPKSWTYGGKRLNATPSRLAMM
                     YEEFQNYDDPATWAPSPPNLENMLNDGYSGFIDGDMGLTWRDYISVWHQNRSHLVYFD
                     GHASLVDAIKFNKKENKCRYAEWLAAGGPM"
     gene            complement(14462..14743)
                     /locus_tag="P5316_06340"
     CDS             complement(14462..14743)
                     /locus_tag="P5316_06340"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRR84614.1"
                     /translation="MECVSASQDRAAIVIVRELLANEGIETVVQNENLSAICGEVAFM
                     RAMPEVCVSRDQDAVRAGAVVERFESGNARDQGHREPWRCPRCGETIEG"
     gene            complement(14873..15724)
                     /locus_tag="P5316_06345"
     CDS             complement(14873..15724)
                     /locus_tag="P5316_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HRR84615.1"
                     /translation="MGIEPQNRMETTSPQEPAEVVPPLCQRPKRAGAEEGGTGEVPLF
                     AGEPAWLTELARRRPDFVFMAPYLAYLLLLPLKDWVPAAYVPWATAARGILGLAVFWF
                     LRRHFPPLGKPHWPIAITAGVLTVVLWVGGEHLLNRVYIGNFHVGGRLFVFPGVFEVK
                     DPREGLTALSWASQVVLRLAVATITVPIVEEIFWRGFLLRAFINWDRFERVPLGAFAW
                     RAFIGTALLSCLQHPDNWVVSILCWLAWNGLMYWKKSLLCLMITHGITNLVLYLYVIR
                     AGDWQFW"
     gene            15996..20810
                     /locus_tag="P5316_06350"
     CDS             15996..20810
                     /locus_tag="P5316_06350"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRR84616.1"
                     /translation="MSRAIPCTRTLFLAFLASFHISAITGGTAGAAEPYLDVVISELM
                     YNPPGTISVGGQSVENEYIEITNRGTQTVDLGGWKIADGVRYTFPGGTLIAPGARLII
                     AKDPASAQTQYGITGVLGPYEGQLDNDGERIELRNADDGLVCELEYDDEAPWPTGGDD
                     DGRSIELIDLSKNGNVGRFWQPSSRIQGSPGMANSPGNPPMTVVINEFVANSAGSGDW
                     IELYNYGNAAVDLNNCYLTDNPRLPTKARISASWSAGTTVIQPKGYWVSTEADWATFG
                     LKTDGEQIYLVASDGITWIDGYNFGNQPIEGASEGRYPDGDKSWYKMVLPTPGYANQP
                     PPPSPVVINEIMYHPPGDSLADPGTEYVELYNTSGTAVDLSGWRLNRAIEYTFAPGTF
                     IPAYGYLVVAYNPASLQSTYGITGVLGPYGKKLSNFSDEIELQDNLRNRVDFVEYRQE
                     GLWPTTPDGCVAPVGPDGCGASLELVNYDMDNRLPGAWRASLGNGTPGQPNSRYIPDP
                     PASIDEVEHAPLVPTASQEVTVTARVGAGNLTSVTLYHKRDADGSFTAVTMHDDGLHG
                     DRLAADGIYGGTIPARTDGTIVEFYIQANAAGGNRKFPDAAPGKTCLYIVQNNPVPDS
                     NLNVFRIVITDENHAGLLSSSNCNAYPPDDTRFDCTFIYGNKAYYNSQIRRRSGARCG
                     PKYSYKIYLPPGYRFRGADRFDLNHEKNDRSFLRNQIVNHLVKYMGLPWARSEYVHTR
                     YRNSYAGVHLYTDSRGQDDFLERHLPTDYDGNLYKATAGWQTATAWNENHGIYQKETN
                     EWLNDWSDLDELGDITSNEPEATYEAEMRRVIDVVNWGRSFAVWGVTCLIDSPWHIHN
                     QNYRLYRRFSDNRFMHILYDFDDTYWDTMWDNALFFNSAYPDVNRYYDCEPLVREYLH
                     GVWRAVNLTNGVYRENRIMPEAHYYHRLIYNDVDLDPVLGSGDNWIAFTNGVNQWSTR
                     LSGRNSLLRANLPSAGLAITTNNGQPITTSNPNLTLAGTAPISAPRVEIAGSETGYRW
                     VDGSVTEWRKDLTLVYMYNTILVRTLNDDGSEIDRRTIDITYTNGVVNDVYFSADVTS
                     GAPPLTVQFTDESRASDITAWYWEFGDGHTSPEQHPTHVYVQDGTYTVRLTITTPGGP
                     LSFERLNYILVQTPPDTVEFTADVTKGAPPLTVSFTDCSTVQGAGSWIWDFGDGRTGS
                     ERNPVHTYTVEGAFDVRLTVTGTQGPSILTKTGYISAFHPPKVAFLVGQVPATVADDS
                     IKRHLESFDLRVDLYDDEPGNRPTAAQIAAEYDVVLASSTILSANVGGEFRHQTVPFI
                     YWEGSLGINGREALADGPGITTSSTRIYVVNNAHPVMAGLPGGGITVTASGTDFSRST
                     GPIASGAQVLARHYSDASQRMVIVAEPGAELLDGGVAAGKRASLYLYDDTWLVTNPTG
                     KKILENAVAWGLGTRTAAFSASVLTGTAPLTVVFTDQSVGPVTSWSWSFGDAATSRLR
                     HPTHVYMIPGTYDVTLTVGGGLGKPASLTRTAYIQVAAPNGPDLDADNDVDASDFAIF
                     RSCLGGPMVAPADPNCLKADFSGDNDVDQEDFGILQRCYSGQGVSPDPACDD"
     gene            20970..22085
                     /locus_tag="P5316_06355"
     CDS             20970..22085
                     /locus_tag="P5316_06355"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRR84617.1"
                     /translation="MREAHRRLLLDLLSLPTSPFHEHAVIAYIRGWAAGRPGISLNTD
                     VYGNLRLDLRRGGRGSTPDWFLSAHMDHPGFAAVRMLDNNRLEARWHGGVQPDFFREA
                     RVRFHTNHRWVRGKIESYEVTNVMGRQRVSTAVVSMSRPVAAEAVGMWDLPNPTIRGS
                     RLFARACDDIAGVAATLAALDILNDLDKPVHVGLLLTRAEEVGFAGAIAACHSRLLPK
                     KVPVLSVECSPAQPGVEMGAGPVVRVGDRMSVFDNAMVAWCRQVAETLSRTTPDFAFQ
                     RKLLDGGACESSAYLEYGHQTTGLSLPLGNYHNMDHRTGRIAPEYIDLKDFDRLICWF
                     VALATAGIRSKPRSPALRQTFDREHRIWGPMLRQSSG"
     gene            22238..23584
                     /locus_tag="P5316_06360"
     CDS             22238..23584
                     /locus_tag="P5316_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRR84618.1"
                     /translation="MLARTLTALLLSAVAAPTVHATETTTPGRLSLRRTPVVEVFEKN
                     RGAVVNISATMEVENYESIFPQGLFNEFFDGMLARPIRRKYTSIGSGFVIHPDGYVVT
                     NAHVVMRAVDQRVIFADGSEFEADRIAVDEKHDLAILKINSGKPCQAVTLGRSDDLMI
                     GETVIAIGNPLEYQHTVTSGIISALNRTLTFSEQVEYKGLIQTDASINRGNSGGPLLN
                     VLGELIGITTAIRGDAQNIGFAIPVDQLHSLLPEMLSIRHRQRLEVGLRLDWHEGVRV
                     SEAKGPAAQAGIRTGDELVSVAGTPIRQDADYYIYLLGITADDSLDMVLKRNSREIKA
                     TVKPATIPIPDAAKLLRQRTGLSVRALTREQAAKIHPQLRAGLQITNVEYGSPAHQAG
                     FVEGYVIVQIGKYAPNNLDEVAVLLESLPAGERVQFRVLEVQKRQILLLQGELAVR"
     gene            24053..25180
                     /locus_tag="P5316_06365"
     CDS             24053..25180
                     /locus_tag="P5316_06365"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRR84619.1"
                     /translation="MRIVVAPDSFKEALPARQVCEAIARGIRRACPDATIDLLPMADG
                     GEGTVDALVAATSGQLRESTVTGPLGEPVRACWGVLGDDSGTAVLEMAAASGLGLVPP
                     DRRDPLLTTTFGTGELIAAALDSGARRILIGIGGSATNDGGTGAAQAIGVRFIDRNGR
                     VLPNGLTGGQLSLIARIDPASRDPRIGPVPIEVACDVDNPLCGPRGAAAVYGPQKGAD
                     PQTVRILDDNLAHLADVIQRDLGMQVRDIPGAGAAGGLGAGLLAFFDAKLRPGIQMVM
                     DALRFSERIAGADLIITGEGRLDRQSMMGKLIEGVGRAAKSAGIPVIALVGSVGDGAD
                     DALEVLQSYHCINPPNTPPAEALPQTATCLEQTAARVLSDH"
     gene            complement(25389..>26192)
                     /locus_tag="P5316_06370"
     CDS             complement(25389..>26192)
                     /locus_tag="P5316_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84620.1"
                     /translation="HRIIKPADLQHVQTGADTETKFVKLGQRPARYFLKELLPEHGKS
                     IEKSISTADDSVPTVYAYTEADLHPFLAYYARLYFKAYTKTIRHSTSNKKSFGEWVHP
                     DVIGVYYAVEDWTAEVLDLSAATGNPAVKLYSFEVKKGLSFSNLREAFFQAVSNSSWA
                     HEGYLVAAEISTDEDFLTELRRLAAAFGIGVIRLALDDPDSSEVLVPARERETLDWDT
                     LNKLTMNNDVTELLTRIKNDLQTKEVIREKYDAVYTAEELVKRIKKKSA"
BASE COUNT         4837 a         8719 c         7735 g         4901 t
ORIGIN      
        1 ggggtcgcag agcagaatgc gatgatacgc gtcaacgccc accagttgtt ccggcgatcc
       61 gggcaccatg gcggcgaagc gcatgcggat gttgtgcggc tgctggcagc cgagaagcat
      121 gccggtgagc atgactacga ggctggcaat gatccgcttt cccatgtcct acactcctga
      181 tgaattcggt tcgacgaaaa gccctcacgc gaaacgtcac ctgcccggcc gcgactcggc
      241 ggccagcgcg ctgcgaagct tatcgagcat ttgtttgaga atgacattgt cggggttgtg
      301 cccgatggcg gtctcgagca cctcgaccgc cttggcgtac cggccctgtt tcacgtagag
      361 gttggcgagc tgcgccggcg gcgccggtag cctgggtgcg agcccgaccg cttcctccag
      421 cagactgatc gcctcctcga ccttattctc ggcggccagg actgaggctt ttgcggctag
      481 ctcgaccgct ctggcctcgg gcgaatgacc ccggagcggc ggcggtgcgg gagcgggctg
      541 ctcgggggcc gagtccgccg gctcctgcgg cggcggcgcg acccgagggc cggtgtcgat
      601 cgtcggctcg ggggccggct cgcgggtcgc cgtccgcgat gcgcgctcca gatcctccag
      661 gtacttgtcg aaacgcggcc ggcgggagtc accctcaggc aaggcggcga gcgcccggcg
      721 catcgccatg cgcgccggac cattctcgtt ctgtgcctga taacaacacg ccagggcgta
      781 gagtatatcc ggcccccacg tgctcgggcc gatcgaagcc gcgtggcgca gcactttgat
      841 cgccgcgtca tgccgaccca gcgatgcctc ggcgctggcc agggcactca agccttggag
      901 cagttgctgg ttgaccggtc gtcgcaccgc caggagtccc cgagccgccc gcgatgcgtc
      961 ggccgctcgc ttggcctccg tgagcagaac cgccgcccgc caagcggcgc caacgtgacg
     1021 ggcattgacg gccagggcgt cgagataggc ggcaatcgcc tcgtcttttc tgttcgcata
     1081 caacagtaac tcgccccggg cgaagtgatg accgtccgcc agcgagcgat cccgatccgc
     1141 cgccagttga tagcagtcga ttgccagatc aagataggcg ggtgacttcg acttcacgta
     1201 gacctcggtg gccgcaatgc ccacgcgggc atacgagaca ccattgagat cgcggggacg
     1261 gccgaggtcg gcgaacttct cggtgacggc ggcccattgt ccttggttaa aggccgcatg
     1321 catttcgcgc aaggcctcgg gcgggccgca gcccacgatg ccaccgagaa tgatcaggca
     1381 acaggtcaca cgacgcatgg atatacctcc cacgacaagc gtcagattta cggactcgtc
     1441 cctcctcgcg gctgtcccgg ctgagtcgcc ggcggtttgt aagtaatcac gcaactggcc
     1501 ttttggcccg gcttgtcaat ccaccagctc tgggtctgcc gggaaatctc gatggcaccc
     1561 ttgaacgtga tcgccgccgt attcggccct tcctcactcg aaaccgattc tatcgtgaag
     1621 atgacctcat cgcgatcggc cgggatcatc agatggaagt ccttgcccgc aatgggcatc
     1681 ttccagccgc tgatgacgcg ggccccgtcc acatagacgt tgatcacgtc gtcctcgagg
     1741 ccaccgttat cccagatgcg gtagtccacc cgccgcgacg ccacggtgat gtcctcgaga
     1801 gtcgacccgg atcgtttgcg ccccttgaat cggaagcgca aaggcttgag atccaacttg
     1861 gcccgcggct gacgaccggc ccgatagaac tccagtatga cctcggcagt gtagagccca
     1921 tcatagtcgt cgctcttgaa ggcgtaggac gcgaagtaga tcctgccctg gttctcccgc
     1981 atgttcacca ccgcaggcag ccgaaggatt gtccggccgg tatcgggact cacccaggtc
     2041 aagctccccc gcaggttgca gtcgacgggt acgtcggtga cgtgaagggc catccgcgcg
     2101 gtgggacgat cggtctcctt caggaactcg taaacgggtg cgcccttcac gttgcccggc
     2161 ggatgctgga tctggtagca gtcctccagg cccttgaaca ggaccttcgg ctcgacgacc
     2221 tggcatcgtc cttcgctctg gaagacgggc gagccgggct tgaggacatc gatgccgtca
     2281 gcgtccttga cgtccaccac ctcgtgacga acgacccagg tgccgcgctc gatcggcggc
     2341 ttgaggctca gcatcttgac cgtgttgtgg ggcccgcctc ccggcacggt gatgcggtcg
     2401 ttgatctgcc accgcagcgt cttgccgctc ggcgaagcca aaatgaagcg gtgggtcacg
     2461 ctgatcagtt ctggcccgta gacttcactg acctccagcg gcgagtcgac ccgcagattg
     2521 gcgtcgtccc agaacttctg gcctgcgagc atccggttgt tctcgcgcac gtcgtagaac
     2581 gtcatcggct tgaacgaagc agcgccaaga ccctcttcct ggacctggaa tgcgatttcg
     2641 ttcttcgcga cttccgtacg ataccgcccg gtcctggaga caacttgcac gtgcaggaag
     2701 gctacgtggt cgccgcggaa gatctgcttg ccgacccagc gaggggcaag ctcaccacgc
     2761 ttcgataggt ccagcacgag cgagctcacc tcatgtttgt caccggcgat cgggagaatc
     2821 tgctggaaga ggcggcggat cttgcctttt tcgatgtagc cggtctcgat gtggaccttc
     2881 atgccgggat agagattgtc gaccttgacc accggcatga gcttgaccgc catggcccgg
     2941 gcgaagaaag tctcgggcat cctcacgtcg ccgtcgcggc ggatgcgctg aagcatgaat
     3001 gtcgacttga actcaaccgc ctggacctcc gcctgctcag ccaggccgtc cagcagacca
     3061 ggatcgatcg gcggcttggt gccgtcgctg ccattatcac cgggttgcga gccgggctga
     3121 gacgctgact ggctggccgc cccaccgggc cggctcgccg cggtcgaggt atcctcgtcc
     3181 cggtcggggc tcggctcgac ctggatgaga tcccagaccg aggtctcgtc gctgagccgc
     3241 tcgagcttga agaaatccgc ctgaggcgcc gggttcttga cgcgggcata gagaagataa
     3301 gtgcccagcg cggcacttga cgcgttccat tcgccgctgc aactgtactc gcctgtgccg
     3361 gcggaaacct ccttggcctc ggcggtgatc gaccggaaac tgctcgcctt gtactggccc
     3421 tgctcctctt ctttgatgta gccgacctcg agcacctcgg gcttcggccc gcccgtcgaa
     3481 gcgagtaccg taaacctggc ggtgccaaac ttcccctggc aaacgatatg ggtgtcgcgg
     3541 atcaggctcg tgccgttcaa caggttcact acgctcttct ggccgtcaag ctcgcgttcg
     3601 aggaccgccg ttgcgaaacg agcctcgggc attggggccg acaccacctc gatcgtgccc
     3661 cgtgccacct caaaatagcg gatgccctcg tccttcgatt cagccacgac gaacggtacg
     3721 tatgtttcga tctcctgcga tacatggcag cggagcttct ctttcgcggg ctgcgtcgga
     3781 tcgggagaat cgatcgtctt ccaggtcgcg acgtcgacca gcgagcgcca gagtcgttcg
     3841 tcgttcggct cgccggaagc cgtcgccccg cccgccggcg gcgcgctgcc cggcgtcgtg
     3901 cccgcgctcg acgcgccgtt gtccgcgctc ggcgccggtg cggggctgga agagaacgtg
     3961 ggctcggcga gcagcgtgtc cgaggagatg agatgacgct gccagcacga gctctcgccg
     4021 agcatgtcga tcaccgcttt cctggggatc agacccacac cgatgcgccg aataccggtg
     4081 tactcgtcgg aggcctggcc cgcgatctca agctcgtatc cgtggcccgc agcgatgcgc
     4141 gtggcgacca actcggcctc ccattcgccc agcggggcat cgggagcatc ggtgtgataa
     4201 acggccacct tgtcaaccag cgccgccccg ccgacgaccg tcacttcgat ctcatccgag
     4261 ccaatcgcct tgcgggcttc gctcgcgtcg tagaccctga ccgagacctt cactttctgg
     4321 cccggggtaa ggtcgctgag cttcagacct accccctccc gatcgccgat gttggcattg
     4381 ctggcgccct gaatcgccca ttcatactcg aacgggcgca ccgcagcgta gcgcggaaca
     4441 gagacctcgg catgcaggtt gagtgttacc tcctggtcga gacggggcac aatgaggaac
     4501 ggcttgcccc ggatctttac cgccttgcct gtgatcggca tggcttggta cttgatccag
     4561 tcgtcggagt cgggcggggg gttggcccgg ctctcggggt agagctgctg gacaatactc
     4621 aaggttgtta cgtcgctgct cggtacggtg ttccgcaaga ccttgtcata catccgccgg
     4681 caaacgtggc cgatcttggc gtcgtcctca aggtcggctt gctggagtcc gcccacctcg
     4741 gcaaggcgct tggtcatgcc ggtgacgtag tcgttgtaga gcatcgatac cagcagatcc
     4801 ttggcgtcct tggactccat cagggcccaa tagtacttgc cgctctggtg aaaccacatc
     4861 cgcgtctcga catcccagag ccaggggttc gcgcacttgc tcatgttctc gaaggtgatc
     4921 cgaccgggga gcggcgtctc gcggttgaga tagatgtcgt ggtagccgcc cgtggcgaag
     4981 ttctcgacgt aatgaaagaa gaagtcgcgg gcgacctcgt gatgcaccgc cagttcaagc
     5041 ggcagggctt tgcgctcggg cggcggcccg cccagtgaat tgacgacgaa ttcggtaagc
     5101 ccggtgcgcg tcagcttcga ctggcctcgc ccgcctcccg ggccaaggag cttggcccag
     5161 ccgacctcct gaatccagcg atccctgatc atggggaaga acgtcgcgta aacgttctcg
     5221 cgtttctcct tcgcatcggc gccgggcacc cacttgagaa cgctgatctt ttcggccggg
     5281 atcacgggtg tcacatatct gggatcaacg ggcggtctca tgccgcggcg atcgagctcc
     5341 gaccagatga acttgtccca tatgctcctg gcctcgccga tcagcttctg tcgccatgcg
     5401 tccatgctcg tccccggcgg cggcgtgtcg ggcaactcgt cgaaccgggc gtactcgggg
     5461 cgcagccact cggcaatgaa aatgtactcg gccatgctga cgccgagctc gtcaagaatg
     5521 cggcggttgg ccagaacgcc ccgacgcacg tcctcgctta aggctctcca cacgtcgtga
     5581 gcgactggca gggcattctt cggcatctct gctgccgact ggcggaaatc gtcggcgtag
     5641 taacctcgac ggtccgtctc gcgaatgcgc tcgacctggg ccgcaatcgt atctcgtgcc
     5701 cggcgggcgg cattcagcat cgaggcagaa agggcaggca cgtcacccgg cttggcgcgc
     5761 aggatccggt caagctcctt gagccggtat tccctgacag ccaggtcggc gctcatgaat
     5821 gcttcgtatt cgggcgtggt ctggttgtcc ttgtagcgct cggcttcgag aaacggcttc
     5881 cacgcgtcgg tggctttgtc gagaagctcc ttgcggtacc tgtccagagg cggagcatcc
     5941 gcgccgcccg gcggatcgtc cggaaagcga ttgataccta ccatctccgg ccggagcatc
     6001 cgcacgtaga gctcccagga gcggggctcc acgccggcct tccacagttt gtcgtcaagc
     6061 tccttgacgc caacgatgcc cgtgccgtcg agcatcatcg gcaaatcggg gagcgtgggg
     6121 aacttcgcga tgacgttgtc gatggtctcc tgggtgagct tcacccaggg gtcatcgtga
     6181 aggagttcag aatagcgcct gctgctgttg tattcctgga gagccttggc tacccggctg
     6241 cggtaggtct ggatcagctc gtcggcaacc ttcacgtagg cctgggcggc cggcttgtcg
     6301 gtctgctggc ggaggtcgag agccaggatg cggtcgagct gcttgagacg gtaacgggct
     6361 ttgttcggat agccggtgag cagccacccc attgtctttt caagatcggg ggcggccttc
     6421 gggaaaaacc agccctccag gctggtcagc ccgtccgcag cggcattgag ctgccgggcg
     6481 gcccactggg ccagcgtcgc cccccatgag tcgggcccct tcgtaggatc aacgccttca
     6541 tagaactcaa gggcggcatt cttgtagatc ccagggccga cgtaggtctc gccgatccgt
     6601 cgtccgaggc cgtaggccgc ccatccttga gccaagctgc ccacgacgtt gcccacggcg
     6661 gccgccgtct cagcagcctg ggcttcagcg taaccggcgt gggtcatggc ccaacgggtg
     6721 aacggctgtg agccccttgt gagcgcaaac atgccgatgt tgctgccggc gctgtaccag
     6781 tcgccggcca tggcctgatc aataagctgc accgcgtcac cgtacacggg tatcgcgtaa
     6841 gcgtactcca ccagcatccg ggccttggcc cgggcccacg ccgcctccgg atcggcggcg
     6901 gcgtccttct tgtattcctc atagacagtc aaggcgcttc ccatcgtgcc caggttgacc
     6961 tgcgacttga agagcgtgct gaactggaac tgcttgggca acccggaggg atcaagcccc
     7021 gccttgcggt acatgctcgc tgtctccgca tccacgtccc agccaaaatg cgtggccact
     7081 tccgagttgg catatcgcgt cgcctcgccc agcaagccgg cggtggtgtc aatggctcgc
     7141 cccaggcggc tgaactcgcc atcaggcgca aagaccttgc tgatccgctc cgaaaacgtc
     7201 ggcacccgct ccgaaacggg tctgtagacc ggcttggcgt ccaaggcttc ctgcacccgc
     7261 tggcgaagct gggcatcggg caagtcgaat ccaaattcct ggataagacg cttcagctca
     7321 gcctgctgcc ccctcacgta gttcgtgacg ctctcagcgt tgtcgtccgc agagccgatc
     7381 cgagtcggag gtggatcctc gatccgggtt agacgagcct ccaagtcgct gcggatcgcc
     7441 cggagacggg ttcgccgggt gcgaaggttg tccatgatcg atttcatcaa agcccggtcc
     7501 tcacggcccg cgtcaaggtt cttgatgact tcatcaaagc gctgggccgc ccgatcaaaa
     7561 ttcgcgtcgt cgagcgcgtt gagagcatgg tgaaactggt tggcccagac gtgcttgaga
     7621 cgccgtgtga ccgtggcccg ggccgtcttg tctcccagaa tctcgacgat ctggttggcc
     7681 cggactttgg cgtccccttc gccttcagtg gcgagcttca ggatacgatc ctgatagctc
     7741 agcgggatgt cgggcgaaat gatcgcgcgg acgcggtcct cggccaccgc gagggctcct
     7801 tgcatcagcg ccgcccgggc ccggtcgccg aagttctctt tggccgcgac gaggatggct
     7861 tcatcggacg gcagtttgcg gatcgcctcc ggaccgtgct gcctatacca ctcgatcatg
     7921 ggcgcgaagg cagcgtctcc cttgttggct cgcagggcgt cagcggtatc gatcagggcc
     7981 tgaaaatgcg cccggctcgc ctcgtcggga aattggcggc cggcgatctc atcgatcatg
     8041 cggcgacgtt ccgcatttgg gatcttgccc cagtccggcg gctcgtacgg gtcgtacttg
     8101 ggcttgccgt tttccaacgc cgaagtcagc atgccctcgg tcacgccccg aagcagatac
     8161 ttggcctgct ccgtcagtgt cgatgcggcc gtcgggtcct tgcccgccgc atggtccgtc
     8221 gccagcagcc aattcccgac catggtgtcg aagccgacct tctggctcag caggggcggg
     8281 atgttcttgt agatcacctc gcgggccgtg actcgctctt cgcggtagac gaatctcggg
     8341 ttgtcggggg ttcccacatc ctcggcggtg atgcggaagt agttctcttc ggcggtgccg
     8401 gtcagggacc ggcggtcaac ctgcgtgccg aatggtgcga atagccagta agcgtccttg
     8461 gtgtgctgaa ggttcttggt ggctcggccg gcgtcttcgt agtaagccgc gtcggcctcc
     8521 ggccgcagcc ggatctccaa cgtgccgccg tcgtcgatca tgccgctgaa caggtcggga
     8581 gggcggttcc tggcatcgtg agccatcacg tccgccacgt ccgggggaac tagatgcact
     8641 tcgaagcggg cgttgaaccg gtcgatcacc ttggcggcgg ggatgatcgt cccgtcgggg
     8701 aatttcacac cgacgaccgt gtggtcgata tcgctggaaa tcagcttggg aaagctgaac
     8761 cagaacaccg acacgtcgcc gccgtggtac tccttggcca cgtcatacat ggccgcagtg
     8821 taagcttcga accgaacgcg attgagcagc tcaccgagtt tctgagagat ggctccacgg
     8881 tcgacgagat acttgacccg cttctggccg ccgtcctcat agatggtgac cagcagcttg
     8941 tgaaatctgt cggcggtgcg aatgttgccc gggatttgca ggagggtggc cttctcggcc
     9001 tgtttgagca actcgtcggg agggctgcgc gtcgtgtctt tggtgaccgc tcgccattcg
     9061 tcgatcagct tctgcttgta ttcctctcga agccgcgtgg tctccgcgtc tacttcccgg
     9121 tcgagctgat ccaggatctt ccggtcctgg tcggcctgcg tgccgtagtt ccattcggag
     9181 ttgacaaagt ccgtgaaggc ccgaatcgcc cagtcgaggg ttttccacct cggctggttg
     9241 tcggttgtcg tctcgcgggg cagttcgctg acccgtgcgg acaggggcac cgaaacgctg
     9301 cccaactgca gcaccgggtc cgggaccccc tcgcggccgg gctcagcggc ccccggatca
     9361 ggcgatgctg ctgccgcggg cggaggcgtt tcgccgggtg tggttgggtc cgcggccggg
     9421 accaccgcga tccatccggc aagccagacg agcgtgccga agagaatcgg gcaggacgga
     9481 caccgtcgtt gttcgaaaag ggcaagaaac ggaagcaggg cagcgtgtcg ccgttgggct
     9541 atcgacatga tctaatcgcc tcccggatgt gctccggtac aagatggggt cgtgcagacg
     9601 actgcaaatg ataaccgaag cagcccgcgt cggccagcct ggcaagccgg tgtacattgc
     9661 tccttgacgt tcttccgggc ttgcgtctat tgtcgcagta tgcacgtcaa agacacttac
     9721 atggtgattg ccgtcagtat cgtcgcttcc gtgggctcgt acgtttgcac acggcccgga
     9781 ttgtggcgac tggcaaagtc gattcctgcg acctcaggcc tgcacaacaa tccgttgcca
     9841 atgcagcttg ggaactgccc cgctgtcggc gaaaatgatc gttctcccaa gtcggtaccg
     9901 aagtccaagc cgatgacctg cgacacgatc ctcggttcga agcctcttgt tttgcggcag
     9961 acgggcgccg acgggcaaac ccggcagtct ccgcatgatc tccaatacgg cggcgcaaag
    10021 gctttgccgg cccagcccgc ggcggtcgct cactggccgc tcaccgccga cgtccgcgac
    10081 gccaccggcc gggggcacga gggtctcgcc cacaacatca cctggaacgc ggacgggtca
    10141 gccgaattca acggccgcga cggcttcatt gagatcccga atcatcccga cctcgcgccc
    10201 ggcacaggtc cctttacgct caccgcctgg gtctacaccg agacagacct tgacgacacc
    10261 ctcggcgaca tcgccggcaa gttcgatccc tcggctcgca agggtttcac actcagtatc
    10321 atgaactacc caggcgtcac ttccggccag cccaactggc gcaacctggc cttcggcatc
    10381 gacgacgcga agatctccca acagtggacc gactgcggtc gccccggcaa ctgcaagatg
    10441 ctctggacat tctgtgtctg gcgagacgac ctttacgccg cgacgttcga actcgaagcc
    10501 gaccaggctg gtcatgtcta ccgctacacc ggcggcacga actgggaaga ctgcggctcg
    10561 cccgacgcgg ccaacgcagt caccgcgctc gccgtcttcg aagacagact ctatgccggc
    10621 accgcccgct acgactccgc cggttcgtcg ttgccctctt cgcccaacaa gaatcccggc
    10681 ggcaaggtct atcgctatga cggtccaggc aaatggacgt actgcggcca actcgagaac
    10741 ccccacaccg gcaaggcgga cacggtccac agtctggccg tctactgcgg caagctctac
    10801 gcctccagca tgtacgccgc aggcaagggc ctttacgagt accaaggtgg atcaggctgg
    10861 aagtatgtgg gacacccggg ctgccgcgtg gaggtcttgg gcgtctacaa cggcgatctc
    10921 tacacgactt cttacgacgc gcccggccgc gtggcccgct ggaaccccac ccgcggctgg
    10981 caggacctcg gcgccccgcc cgacacgagc cagacctacg gcttcatgcc gtacgaaggc
    11041 cggctcatgc tcgcaacgtg gcccagagca actgtctact gcctcgatga ggcggatcag
    11101 tgggcctgcc agggccggct tgacgaagag agcgagacca tggccatggt cgtttacaac
    11161 ggcaagctgt acgccggctc cctgccgctg gccaaggttt atcgttacga tggcggccgg
    11221 acatggaccg agaccgggcg cctcgatctc acggccgacg tacgctaccg tcgcgtctgg
    11281 tcgatggccg ttttcaaagg caagcttttt gccggcacga tgccctccgg ccatgtctgg
    11341 tgctttgagg ccggccgcaa cgccacctgc gatcacgaat tgccccccgg ttggcaccac
    11401 gttgccgctg tccgcgacga gaaccggctt cgactccatg tcgatggctc gctcgtctcc
    11461 cgatccgaac ctttcaggct cgacgatttc gacctgagca ataattcgcc cttacgaatt
    11521 ggactgggct cacacgacta cttccgcggc cgcatggcgg acgttctttt ctaccgcgcc
    11581 gccctgtccg acgacatgat cgcagccctc gcccgccgtc ggccccgacc gaccacccgc
    11641 ccgtagcctt cgcacctgcc aagagcgtcg tccgtactgc ttgcgatgat cagacctcct
    11701 ccacaacctt gagccccatc aggtgggcca ggtgacggat ggtccgggtg tggttgccgt
    11761 agtaggccac tcggtgcagc gaggcatagt aatccttgac gctagccccc agcgccccgc
    11821 cgccccaatt ggccagcagc ttgtccgcat ctttgacctc ggcgacgagt tccgtccggc
    11881 agcctcgctc ggtgcccttg gggacttcgg gaacctcgac aatcttcccc gtgaacagca
    11941 gcagcgtgtc gaggtggacc agcttggtga acgtgacttc ctggccgatg cgataatgga
    12001 cctcgggaac cgctccaccg cggacctcgg cgtgccgacg tatcaggtag ggtgcggccg
    12061 gactctcggg tccctccatc ctcagcgggg ccgagcagtg ggaaaggtga aagacatttc
    12121 tggctgtgtc gacgcctccc gcgttgccca ggaacccggc ccggttcaac gcgtaccgga
    12181 agatcagcat ggtcagcaga cagtccatgt ccccgtcgca cgcggccggg atcccacgat
    12241 cgttcagccg tgaaaacccc aggcatggaa aacctctcgg caaccagccc atgcatgtcc
    12301 cgacgcacag cccgtcggcc cggctttcgg cgatgagttg atcaagcgtc aaactcgccc
    12361 gcgcggcctt caggacgtct tcgcgcgtcg gctcgaccat accctttgcc tcgtccagcc
    12421 atcgctgggc ctctttttcc gttgcggcag catccacccc ggccatgacc tcgtcgtaac
    12481 gtccgtcggc aatggccacg atttccactc ccagccgcga tttcacttgt tccgggtcgt
    12541 gggaggcggg cgttcccttg aacggcggag aagcgagcac ccggctttgc cgcaacaacg
    12601 ggatcaccct cagcaacgcc gccgcccgct ccagatcgtc gtaatcgctg ctgttgaaca
    12661 gcgtgatccg gtgcccttcc tcgcgccagg ggttcaccag gcaccagtcg tgcccgcccg
    12721 taacgggcac atgaaagacg gccacgggcc ggccttcctt gaagaccgtg ggcatcacgc
    12781 ccctgctcag cccaaagatg tccgcgctga ccgccagcac gggagcgtcc ggacctgatt
    12841 tggccagcaa ctgagtggtc tcttcaacgg tgctcgcctg accgatgacg aactccacgt
    12901 tgccaagatt cgcctccgcc tcggccaggc gcttccgcac cgccgcgatc tctgtttccg
    12961 agctgcccca actgcgatcc gccggagtac ccgcaatcac tacgcagatc cggaccttcg
    13021 atctcaccga tgacggcgtt cccgacgcct cggccccggc cgccaagcga cctgccgcca
    13081 acgcgatccc gcttcctaca cctgcctcga gtagctcccg acgagtgcaa tgcatacaca
    13141 tgagcagttc ctccgtagat gcatggttgt cctggagaat gcacgatcgc ccttgcctgc
    13201 cgggaatccg gccgcaggcc gcgcaagacc gggcgcctgc caactcggaa ctccggaact
    13261 gccgaggaaa ggacgtcccg ttggccggac ttcgatcgcg aatcctaccc gaatgacctg
    13321 ttccatccaa gcaatggcgg cctgtcacac catgatgctc gtttcctcgc ccccgcgacg
    13381 tagtgctgtc ttcgctgcgt cagttgtcca tcgccgcgta agccgcccgt ggcatttctt
    13441 gagcaggcga aagcccgcaa ctgaaaaggg cagtagcgcg gtcatccact ctcggcatgc
    13501 tacatgggcc cgcctgcggc cagccactcg gcgtacctgc acttgttttc tttcttgttg
    13561 aacttgatcg catccaccag gctggcgtgt ccgtcaaagt agacaagatg tgagcgattc
    13621 tggtgccaga cgctgatata atccctccag gtcagtccca tatcgccgtc aatgaagccg
    13681 ctgtatccgt cgttgagcat gttctccagg ttgggaggac tgggcgccca ggtggcggga
    13741 tcatcgtagt tctggaactc ctcgtacatc atggcgagcc gactcggcgt ggcattcagg
    13801 cgcttgccgc cgtaggtcca gctcttgggg agccgatccg gatcgatgaa atccgagcgg
    13861 tacatcgccg ggagcgaacc attgtcgatc ttgtcggcga tcgatccgat gtgcgcggca
    13921 tagaactggt gaggatgaat caaacgcaca taactgtgaa tcggcttgtt gccccggaac
    13981 gctccgccga ccgtctggcc gctgccgatc gacgcggccc tgcgagtgag cgtatcgtcc
    14041 gggcagaagt aagcttgggc gttcttcacg tatttgaaaa tgtggccgaa ccggacccat
    14101 tgggcggagt ccgcgggccg cgacccgccc ttgtacaggt tcggaaacca attcaccatc
    14161 tcgccgccga tgccgtccca caaccaccag tccgggtccg ggaaatgctg cttatggtca
    14221 atcgcgtaga aggcgtgccc cttgtacatg gtgtccaggt tcgtcttgca caccgtgatt
    14281 ttggccacct gtctggcccg gttcagcgag ggcaacagga tggcaatcaa aagagcgagg
    14341 atggccgcca cgaccagcat ttcgatcagc gtgaacgcat tcctggatct ggagcacatg
    14401 ttctcacctc gcctgatcat gcggtctcca tctgtgtacc acatttccgg caggcggtga
    14461 actacccttc aatcgtctcg ccgcaacggg gacatcgcca cggctccctg tgaccctgat
    14521 cccgcgcgtt ccccgactcg aaccgctcca cgacggcccc tgcgcgtacc gcgtcctgat
    14581 cacgcgaaac gcaaacctcg ggcatcgcgc gcatgaacgc aacctccccg cagatggcgg
    14641 acaggttctc attctgaaca acagtctcta tgccttcatt cgcgagcaac tcgcgaacaa
    14701 tgacgatcgc ggcccggtcc tgagatgcgg agacgcactc catgtctcct attctcgttc
    14761 ttcggtcctc cgcgtgtcaa tcgaaatccg gaacgagcaa tccgtccgag ccgcgtatga
    14821 tttccgcgtc ctttcaatga aagccaccgg tgaagccttg ctgagcctgt tctcaccaga
    14881 actgccagtc gcccgcccgg atcacgtaga ggtacagcac cagattggtg atcccgtgtg
    14941 tgatcatcag gcacagcagg ctctttttcc agtacatcag cccgttccag gccagccagc
    15001 aaagaatgct gacgacccag ttgtcgggat gctgaaggca cgacagcaag gccgtcccga
    15061 taaaagcccg ccaggcaaat gcgcccagcg gcacccgctc gaagcggtcc cagttgatga
    15121 aagcccgaag caggaagccg cgccagaaga tctcttcaac gatcggcact gtgatcgtcg
    15181 caaccgcaag gcgcaacacc acctgcgacg cccatgagag cgcggtcagg ccctcgcgcg
    15241 ggtctttgac ctcgaaaacg cccggaaaca cgaagagccg cccaccaaca tggaagttgc
    15301 cgatgtaaac ccggttcagc aggtgctcgc cgccgaccca gagcaccacg gtcagcacgc
    15361 ccgccgtgat cgcaatcggc caatggggct tacccagcgg cggaaagtgg cgacggagaa
    15421 accagaacac cgccagcccc aaaatgcccc gggctgcggt ggcccagggc acataagcgg
    15481 ccggaaccca atccttcaga ggcagaagca gcagatatgc caggtaggga gccatgaaaa
    15541 cgaagtcggg gcggcggcgg gccaactccg tgagccaggc aggttcaccc gcaaacagag
    15601 gaacctcccc ggtgcctccc tcctcagctc cggccctctt aggtctttgg cagagaggtg
    15661 gcacgacttc ggccggttcc tgcggcgagg ttgtctccat gcgattctgt ggctcaattc
    15721 ccatagactt ctccggtcaa tctgcttcca aggcaaaccg catccttgcg ggccgcagcc
    15781 aaaacgcatt gtgagccgga aacatgcgcc cggaaagaat acccgtcccg acacaaggga
    15841 cgagcaacgc ccgtgtcgcc cgctctgccc ggcgcccgac cggtaagtat aaggtcaaca
    15901 cctccagacc aggaaaccag gcgggcctct atggtatact acccgggagc ggagtctccg
    15961 ccggccccgc gccgggcaaa ccaggaggtt aacgaatgtc tcgggcaata ccctgtacgc
    16021 gtactctttt cctggccttt ctggcctcat ttcatatatc cgctattacc ggcggcacgg
    16081 caggtgccgc cgagccctac ctcgacgtgg tcatcagcga gctgatgtac aaccccccgg
    16141 gaacgatatc cgttggcggc cagagcgtcg aaaacgagta tatcgagatc accaatcgcg
    16201 ggacacagac ggtggacctg ggcggctgga agatcgccga cggcgtccgc tacaccttcc
    16261 cagggggaac cctgattgcc cccggtgccc ggctgatcat cgccaaggac ccggcctcag
    16321 cccagacaca gtacggaatc accggagttc tcggacctta cgagggtcag ctcgacaacg
    16381 acggcgagcg aatcgaactg cgcaatgcgg atgacggttt ggtatgcgag ctcgagtacg
    16441 acgatgaagc cccctggccg accggtggag acgatgacgg ccgatccatc gagctgatcg
    16501 atttgagtaa gaacggcaac gtaggacggt tctggcagcc ctcttcgcgc atccagggct
    16561 cgcccggaat ggccaattcc cccggcaatc cgccgatgac cgtggtgatc aacgaattcg
    16621 tggccaactc ggccggctcg ggcgattgga tcgagttgta caactacggc aacgccgcgg
    16681 tcgatttgaa caactgttat ctgaccgaca accccaggct gccgacgaag gcccgcatct
    16741 ccgcttcatg gtcggcagga acgaccgtca tacaacccaa gggatactgg gtctcaacgg
    16801 aggcggactg ggccaccttc ggcctcaaga ccgacgggga acagatctat ctcgtcgcct
    16861 cggacggcat cacctggatc gacggctaca acttcggaaa ccagccgatc gagggtgcct
    16921 ccgaagggcg atatcccgac ggcgataaga gctggtacaa aatggtcctg ccgacgcccg
    16981 gttatgccaa tcagccgccg cctccgtccc ccgtggtcat caacgagatc atgtatcacc
    17041 cgcccggcga cagcttggct gacccgggga ccgagtacgt ggagttgtac aacacctccg
    17101 gcacggccgt tgacctgagt ggttggcgac tcaaccgggc gattgagtac accttcgcgc
    17161 ccggcacctt catccccgcg tacggctacc tggtcgtggc ctacaacccg gcttcgcttc
    17221 aaagcaccta cggcatcacc ggcgtgcttg ggccttatgg caagaagctg tccaacttct
    17281 cggacgagat cgaactccag gacaacctca gaaaccgcgt cgatttcgtc gagtatcgcc
    17341 aggaaggact ctggcccacc acacccgacg gttgcgtcgc cccggtcggc cccgacggtt
    17401 gcggagcctc tcttgaactg gtcaattacg acatggacaa ccgcctgccg ggcgcctggc
    17461 gcgccagcct cggcaatggc acccccggtc aaccgaacag ccgctacatc ccggacccgc
    17521 cggcctcaat cgatgaggtc gaacatgcac cattggttcc cacagcaagc caggaagtga
    17581 ccgtcacggc acgcgtcggg gccggcaacc tgacctccgt gaccctctat cacaagcgag
    17641 atgcggacgg ctctttcacc gccgtcacca tgcacgacga cggcctgcat ggagatcggc
    17701 tggccgccga cggcatctac ggcggaacga tcccggcacg aaccgacggc accatcgtgg
    17761 agttctacat ccaggccaac gccgctggcg gcaaccgcaa gttccccgac gcggcacccg
    17821 gcaaaacgtg cttgtacatc gtccaaaaca acccggtgcc ggactcgaat ctcaacgtct
    17881 tccggattgt catcaccgat gagaaccacg cggggctgct cagttcatcg aactgcaatg
    17941 cctaccctcc ggatgacaca cgattcgatt gcaccttcat ctacggcaac aaggcttact
    18001 acaactcgca gatccgtcgc agaagcggag cccgctgcgg ccccaaatac tcgtacaaga
    18061 tctacctgcc ccccggttac cgcttccgcg gcgcagatcg cttcgatctc aaccatgaga
    18121 agaacgatcg cagcttcctc cgcaaccaga tcgtcaacca cttggtaaag tacatgggcc
    18181 tgccgtgggc gcgaagcgag tatgttcaca cccgctaccg caacagctat gcaggggtgc
    18241 acctgtacac tgattcccga ggccaggacg actttctgga acgacacctg ccgaccgact
    18301 acgacggcaa cctctataag gccaccgcag gatggcaaac cgcgacggcc tggaatgaaa
    18361 accacggcat ttaccagaag gaaaccaacg aatggctcaa cgactggagc gatctggatg
    18421 agttgggcga catcacctca aatgagccgg aggctacgta cgaagcggag atgcgacggg
    18481 tcatcgatgt cgtcaactgg ggccggagct ttgccgtctg gggcgtgacc tgtctgatcg
    18541 actctccgtg gcacatccac aaccagaact accgcctcta ccgccgattc tccgacaacc
    18601 gcttcatgca tatcctctac gactttgacg acacctattg ggacacgatg tgggacaacg
    18661 ctctgttctt caactccgcg tatcccgatg tcaatcgcta ctacgattgc gaacccctgg
    18721 tccgtgaata cctgcacggc gtctggcggg cggtgaatct gaccaacggc gtctaccgcg
    18781 aaaaccggat catgcctgaa gcccactatt accatcgcct gatctacaac gacgtcgatc
    18841 tcgatccggt gctcgggtcc ggcgacaact ggatcgcttt cacgaacggt gtcaaccagt
    18901 ggagcacgcg cctgtccggc cgcaactcgc tgcttcgcgc gaacctgccc tcggccggct
    18961 tggccatcac caccaacaac ggccagccga tcaccacgag taacccgaat ctgaccctcg
    19021 ccggcaccgc cccgatcagc gccccgcgcg tcgaaatcgc cggctcggaa accggttatc
    19081 gctgggttga cggctcagtc actgagtggc ggaaagacct gacgttggtc tacatgtaca
    19141 acaccatcct ggttcgcacc ctgaatgatg acggcagcga gatcgaccgt cgaaccatcg
    19201 acatcaccta taccaacggc gtggtgaacg acgtttactt cagcgcggat gttaccagcg
    19261 gagcgccgcc gctgaccgtt cagttcaccg acgagtcgcg ggcttcggat atcaccgcct
    19321 ggtactggga gttcggcgac ggtcatacca gccccgagca gcacccgacc cacgtctacg
    19381 tgcaggacgg cacctacacg gtgcgactga cgattaccac ccccggcggg ccgttgtcct
    19441 tcgagcgtct taactacatc ctcgtacaga ctccgcctga tacggttgag ttcactgctg
    19501 atgttaccaa gggagcgccg ccgctcaccg tcagtttcac cgattgctca accgtgcagg
    19561 gcgccggctc ctggatatgg gactttggag acggccggac cggcagcgaa cgtaacccgg
    19621 tgcataccta caccgtcgaa ggtgccttcg acgtccgctt gacggtgacg ggcacgcaag
    19681 gtccatccat cctgaccaag accggctaca tcagcgcatt tcatccgccc aaggtcgctt
    19741 tcctggttgg ccaggtcccg gcaacggtcg ccgacgactc gatcaagcgg catctcgagt
    19801 cgttcgacct tcgcgtcgac ctttatgacg acgaaccggg caaccgtccg accgccgctc
    19861 agatcgccgc tgaatacgac gtcgtactcg cgtcctcgac gatcctgtca gccaatgtgg
    19921 gcggcgagtt ccgccaccag acggtgccgt tcatttactg ggaaggctcg ctgggcatca
    19981 acggacgcga ggcactggcc gacggccccg gcatcaccac gagttccacg cgcatctacg
    20041 tggtgaacaa cgcgcacccg gtcatggccg gtttgcctgg cggcggtatt actgtgaccg
    20101 cctcgggaac cgacttcagc cgaagcacag gccccatcgc ttcgggcgcg caggtcctgg
    20161 ccaggcatta cagtgacgcg agtcaacgca tggtcatcgt ggccgagccg ggcgcggaat
    20221 tgctcgatgg cggcgtggcg gccggaaaac gggcgtcact ttacctgtac gacgatacgt
    20281 ggctggttac caaccccacc ggcaaaaaga tacttgaaaa cgccgtcgcc tggggcctcg
    20341 gaacacggac tgccgccttc tccgcgtcgg ttctcactgg aaccgctccg ttgacggtcg
    20401 tattcaccga ccagtccgtc ggtcccgtca cttcgtggag ctggagtttc ggcgacgcgg
    20461 cgacgagccg gctcaggcat ccgacgcacg tctacatgat tcccggaacc tatgacgtca
    20521 ctctgaccgt gggcggcggt ctcggcaaac cggcatcgtt gacccggacg gcctacattc
    20581 aagtcgcggc cccaaacggc cccgacctcg acgctgacaa cgacgtcgac gccagtgatt
    20641 tcgcgatctt tcggtcctgc ctcggcggtc caatggttgc ccccgctgat ccgaattgcc
    20701 tgaaggccga tttcagcggc gacaacgacg tcgaccagga ggatttcggc attctgcagc
    20761 gatgttacag cggccagggc gtcagccccg accccgcctg cgacgactga gccgcctttg
    20821 cgggttccct tttggtttgg cgacggtaat caagccgggg ggaccccgcc tcgttttcga
    20881 ggtgcgggag gaatgaacgc agaccatccc actcgtcgcc tgcccccggc ggcttgcccg
    20941 ccggtgcatc actgcatagg ctgctggaca tgcgagaggc tcatcgccgc ctgctgctgg
    21001 atttgttgtc cttgccaaca tcgccctttc acgaacacgc cgtgatcgcc tacatccgcg
    21061 gatgggccgc cggacggcca ggtatcagtc tgaacaccga cgtctacggg aatctccgcc
    21121 tcgatctccg ccgcggcggt cgaggctcca caccggactg gtttctctcg gcgcacatgg
    21181 atcatcccgg ctttgccgcc gtccgaatgc tggacaacaa ccgccttgaa gcaaggtggc
    21241 acggcggcgt ccagccggat ttcttccgcg aggcccgcgt acggttccac actaatcacc
    21301 ggtgggtccg cggaaaaatc gagtcctacg aggtgaccaa cgtcatgggc cggcagcgcg
    21361 tttccacggc cgtcgtctcg atgtcccgac ccgtcgccgc tgaagccgtc ggcatgtggg
    21421 acctgcccaa tccgacgatt cgtgggtccc gcctgtttgc ccgcgcctgt gatgacattg
    21481 ccggcgtggc cgcaaccttg gccgcactcg acatcctgaa cgacctcgac aaaccggttc
    21541 acgtgggtct gctgctcacc cgtgccgaag aggtgggctt cgcaggtgcc atcgccgcgt
    21601 gccacagcag gctgctgccg aaaaaggttc ccgtgttgtc cgtggaatgc agtccagccc
    21661 aacccggcgt ggagatgggt gccggtcccg ttgtccgcgt gggcgaccgc atgtcggtct
    21721 tcgacaacgc tatggtcgca tggtgccgcc aggtggccga gaccctatcc cgcacaaccc
    21781 ccgatttcgc ctttcagcga aagctccttg acggcggggc ttgtgaatcc tccgcctacc
    21841 tcgagtacgg ccaccagacc accggcctga gcctgcccct gggcaactat cacaacatgg
    21901 accaccgcac cggccgcatc gcccctgagt acatcgatct caaggatttc gaccgactca
    21961 tctgctggtt cgtcgccctg gcaacggcgg gaatcagatc gaaaccccgc tcacctgccc
    22021 tgcgccaaac gttcgaccgc gaacatcgca tatggggacc aatgctccgg caatcatccg
    22081 ggtagggcgc tgtggaggtc gccccggcca ccgagacctg ccttggtggc tgcccgatct
    22141 ccgccgcgtc actccttgtt ctcaactcct cgttcccaat tccttcggcc gtgttatact
    22201 cattcgcacc gaagactcga ataaggcagg tggttccatg ctcgccagaa cactgaccgc
    22261 tttgctgttg tcggcagtcg cggcccccac ggtccacgcc accgagacga caacgcccgg
    22321 ccgcctttct cttcgtcgaa cgcccgtcgt cgaggtgttc gagaaaaacc gcggcgcggt
    22381 ggtgaacatc tccgcgacca tggaggttga gaactacgag agcatcttcc cccagggcct
    22441 gttcaacgag tttttcgacg ggatgcttgc tcgccccata cgccgcaagt acaccagcat
    22501 cggcagcggg tttgtcatcc atccggacgg ctacgtggtc accaacgccc atgtggtgat
    22561 gcgggccgtc gaccagcgcg tgatcttcgc cgacgggtcc gagttcgagg ccgatcgtat
    22621 cgccgtcgat gagaagcacg acctggccat tctcaagatc aactccggca aaccctgcca
    22681 ggccgtcacc ctcggccgaa gcgacgacct gatgatcggc gagacggtca tcgcgatcgg
    22741 caacccgctc gagtaccagc acacggtcac ctctggtatc atcagtgctc tgaaccgcac
    22801 gctgaccttc agcgaacagg tcgaatacaa gggcctgatc cagaccgacg caagcatcaa
    22861 tcgcggcaat tccgggggcc cgctgcttaa tgtcctcggc gagctgatcg gcatcaccac
    22921 cgccatccgc ggtgacgccc agaatatcgg ctttgccatc cccgtcgacc agttgcatag
    22981 cctgctgccc gagatgctct ccatccggca ccgccagcgg ctcgaggtcg gtctgcgcct
    23041 cgattggcac gaaggtgtcc gcgtgtcgga agccaagggc cccgctgccc aggccggaat
    23101 aagaaccggc gatgagctgg tcagcgtggc cggtacgccc attcggcagg atgccgacta
    23161 ctatatctat ctgctgggca tcacggctga tgattcgtta gacatggtcc tcaagcggaa
    23221 ctcgcgtgaa atcaaggcca cggtcaagcc ggcaaccatc cccattcccg atgccgccaa
    23281 gctcctccgc cagcgaacgg gcctgtcggt ccgcgccctg acccgcgagc aagccgccaa
    23341 gatccacccg caactgcggg ccggcctcca gatcaccaac gtcgagtacg gcagccccgc
    23401 ccaccaggcc ggattcgtcg agggctacgt catcgtccag atcggcaagt acgcccccaa
    23461 caatctcgac gaggtggcgg tgctcctgga aagcctgccc gccggcgagc gcgtccagtt
    23521 ccgcgtgctc gaagtgcaaa aacgacagat cctccttctc caaggcgaac tggccgtccg
    23581 ctagcgggtg tcccatcctc gccgcagcga cggtggggtc acgatgatcg attacgcggg
    23641 gatcacgtca acggctccgc gtggacatgc tctctccctc agctaagagc acacagtagg
    23701 gcagcctcga ggtcgccccg gcgaccgaga cctgccgcat tgaagctggc cccagaatcc
    23761 attctgggac ctttcgcata gacgattccc atccccattc tcaccgaatg cggaatggac
    23821 tccgccgggc aaggcagccc cgcttcgcaa tcagcgcttc gcaggacagg cccggaacgg
    23881 attccgggct aagccccccc acccagctaa ccgccaaagg ctgagagtca cggcgtagcg
    23941 attccttttc cgcccactga cgactgacat ctgataaccg atcactcgcg ccgctgcccg
    24001 ctcctcgacc gcctgtagcg gacctcaacc ccccacaggt atattgctga gcatgcgaat
    24061 cgtcgtcgcc cccgactcat tcaaagaggc cctgcccgcc cggcaggtct gcgaggccat
    24121 cgcccgcggt atccgtcgtg cctgccccga tgcaaccatc gatctgctgc cgatggccga
    24181 cggtggcgag ggcacggtcg atgctcttgt ggcggccacc agcggccaac tgcgcgaatc
    24241 aaccgtgacc ggcccgcttg gcgaaccagt gcgggcctgc tggggcgtac tcggcgacga
    24301 cagcggaacg gccgtgctgg agatggccgc cgcatccggc ctggggctgg ttccgcccga
    24361 ccgccgcgac ccgctgttga ccaccacctt cggcacaggc gagctgatcg ccgccgcctt
    24421 ggacagcggc gcccggcgga tcctgatcgg catcggcggc agcgctacca acgacggcgg
    24481 caccggcgcc gcccaggcga tcggcgtacg attcatcgac cgcaacggcc gcgtgctgcc
    24541 caacggcttg accggcggtc aactgagcct catcgcccga atcgatccgg cctctcgcga
    24601 cccgcgcatc gggcccgtgc cgattgaggt cgcctgcgac gtcgataacc ccctgtgcgg
    24661 ccctcgcggt gcggccgccg tctacggccc gcagaaaggc gctgaccccc agaccgttcg
    24721 cattctcgat gataatctgg ctcacttggc ggacgtcatt cagcgcgatc tgggcatgca
    24781 ggttcgcgac attcctggcg ccggcgccgc cggcggtctg ggcgccggcc tccttgcatt
    24841 cttcgacgcg aagctacggc cgggaatcca gatggttatg gacgctttgc gcttctccga
    24901 acggatcgcc ggcgccgacc tgatcatcac cggcgaaggc cgcctcgacc gccaatccat
    24961 gatgggcaag ctgatcgagg gcgtcggccg cgccgccaag tcagccggca tacccgtcat
    25021 cgccctggtc ggttctgtcg gcgacggggc cgacgacgcc ctcgaagtcc tccagagcta
    25081 ccactgcatc aacccgccca ataccccacc ggccgaggcc ctccctcaaa ccgccacctg
    25141 cctcgaacaa accgccgcga gagtgctgtc agatcactga caaccgacgg ttgaccgctt
    25201 tccccaaaca atgccgacga ctggatgcca tttgttgacc gctgaatact gcgaagccgg
    25261 aagcagagcc gaggcgcgcc aacacgaatg cttacccgtt tcaggaggca caaagcaccc
    25321 tgcaagaaat ccattgtctt cttcgttggt tcctgtggtc caacactgtt cgcgatcggc
    25381 gcggatcatc aggcagactt cttcttgatt cgtttcacga gttcctcagc cgtatagaca
    25441 gcgtcgtatt tttcgcgaat tacctctttc gtctgaagat catttttgat ccgggtcagc
    25501 aactcggtga cgtcattgtt catggtgagc ttgttgagcg tgtcccaatc cagcgtctca
    25561 cgttcccggg ccggcacaag gacttctgag ctgtctggat catccagagc aagtcgaatg
    25621 acccctatcc caaaggcggc agccagcctg cgcaactccg tcaggaagtc ttcgtcggtc
    25681 gaaatctccg ccgcaacaag gtatccctcg tgagcccatg acgagttcga gaccgcctgg
    25741 aagaaggcct ccctgagatt ggagaatgaa agccctttct tgacttcaaa cgagtatagc
    25801 ttcactgctg gattgccggt tgcggcacta agatcgagca cttcggcggt ccagtcctcg
    25861 accgcgtagt agacaccgat gacgtcggga tgtacccact caccgaacga cttcttgttg
    25921 gatgtgctgt gtctgatggt cttcgtgtag gccttgaaat acaaacgcgc gtagtacgcg
    25981 aggaagggat gaagatcagc ttcagtatac gcatagaccg taggcacgct gtcatccgct
    26041 gtcgagatgg acttctcgat actcttaccg tgctcaggaa gcaattcctt aaggaaatag
    26101 cgagccggtc gctgccccaa cttcacaaac ttcgtctcgg tgtcggcgcc ggtctgaaca
    26161 tgctgtaaat ccgccggttt gatgatgcgg tg
//
LOCUS       DAOTPW010000049        26147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1940_length_26147_cov_5.671125, whole genome shotgun sequence.
ACCESSION   DAOTPW010000049 DAOTPW010000000
VERSION     DAOTPW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26147
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1940_length_26147_cov_5.671125"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..1164
                     /gene="dapF"
                     /locus_tag="P5316_06375"
     CDS             307..1164
                     /gene="dapF"
                     /locus_tag="P5316_06375"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRR84621.1"
                     /translation="MKFTKLHGLGNCYIYVNCFDERVNDAPSLARAVSDRNTGVGSDG
                     LILICPSRTADARMEMYNVDGSRGQMCGNGIRCLAKYVYDHGLSRNNPLRIETDAGIK
                     TVSLNIEDGKVASACVDMGEPRLRPEDLPVKLTQEQMIDHPLEILDHQALMTCVSMGN
                     PHAVIYGVPLKLLNNRGPAGWNLEIEGRRIETAAIFPERTNVHFVDVLHPSEVKMLTW
                     ERGSGATQACGTGASAVCVAGVLTKRSSRKIIAHLPGGDLTLEWREADNHVYMTGPAV
                     EIFTGDWPA"
     gene            complement(1199..3391)
                     /locus_tag="P5316_06380"
     CDS             complement(1199..3391)
                     /locus_tag="P5316_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR84622.1"
                     /translation="MRSSTRSRRKSRPPLLTTILVVGAFVGVQVATRDQHPEASAGTG
                     RLRPLVVSSPHPGRSADTAQLTLIDALIEARQDPHDNVIQFDPKVFKSSELSIRLPEA
                     LVISDESGGHDRIDGALPDGLVTLDSSDCPDAGIMVSGKAQISLMNLAVRGGRLRGIL
                     ISANARLNLDNVLVRDCLGPGVAVFGEARVHIRGGSINTNGSHGVEVRDRALADLEDV
                     RLSGNGQSDVAGFDQGGIFAAGCQMSSAADWNVVLSDEAQAILTRCALSRARFANADA
                     SERAKLRIADSTIDESERTGIFATGQAVIDLTSTRLRRHSSRCIELQDEAAATIEASA
                     MEFGGEYGMVLFGKSSVHASQVRITDSGSHGVCLRQQARGWFDRCVFTGNRRSGIACP
                     DAYEGGPVRASRCIFSGNGMRPICRGPLHISPLAPTPVRIDGPQVTCTAEPNATVELY
                     ADRVGEAARFLRTVSADGMGRFTVDCRDVPAGHVITAAATSAESTSEFNVVAGADCGP
                     ILSALLGDTGPLSDKGGPIRPESLIRRWEADTRLIFNVVNPPNSAVERYVRFLVDRIN
                     DWTGASLRSELGIGQMRRPPANAVVLPIRYVTADSPALVGRGGATFMKWDAGGLFMPP
                     MEVVLAVGQDPRQTCPRVLAHEIGHTLGLCHARVGLLSRMQGITPPPKDSSYLNDFSP
                     MMTYYDVLALQILHDRRNSGRLTVGQLVEKGTISTDAGGVLDSGTVSR"
     gene            3724..5697
                     /locus_tag="P5316_06385"
     CDS             3724..5697
                     /locus_tag="P5316_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR84623.1"
                     /translation="MYPARMTRVLLLTVDEGLADVVRGICRQRSFEYVVLPGLPSAKT
                     PKDYFDLVLVDARPAPTSTLSFNTIPPITLEKTAPLEAGRVLAISHYGTLVGRMTGYV
                     SGASGYCRIHPFGISCDPALSEQLTEAILDGLSLPEYRRVDFHDAFPDRDFYRQVFES
                     IQIPIVLITSEGIIKLMNLRALARFNYFTQMVINQSWLMLVAPQDRGKRAEDFLVRIS
                     GGYFYEGTLRCVDRAGKEFPGLITSSRVFAPTHGSDMLIILAIHDLTEMEELQRQVTT
                     FQKMESMERVVAGLTHEFNNMLTAILGHAEMLTQDLPRGTDLYDSARVICQEAERARE
                     MTARLLGLSSSRQFVPGRVSCNQVVRETQALLRHSLGDAVRIETCLMESGDAVDGDAS
                     QLRQVLVNLCLNARDAMNGKGVITIRTSLREVTPEECGPHQDWTPGMFVEICVQDQGC
                     GIAPDIIGRVFEPFFTTKQDGAGTGLGLSVVRGIVRTHGGHVTIESKVGQGTSVRVRL
                     PQREAQAEEADRADENPPAAVPCGKGACVLIVDDDRAVLTYAQKALQRAGYKVRMATH
                     GLLALEIFEHYRDEIELIMLDLTMPGTAGRDILKSLRKMDAKVPIVICTGFAHGGMDD
                     DLLSQVQGFIKKPFRPNDLIEQVAEVLQAAREE"
     gene            6000..6344
                     /locus_tag="P5316_06390"
     CDS             6000..6344
                     /locus_tag="P5316_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRR84624.1"
                     /translation="MSEQDKKQSGGDEGTCNAVPVKPRRERLPRQSPPRVLPPWKVIL
                     HNDDVNEMNRVVEVICLLTRLNRQEAAARMEEAHKTGAALLLVTHRERAELYVEQFAT
                     YNLTVTAEPEVG"
     gene            complement(6429..6647)
                     /locus_tag="P5316_06395"
     CDS             complement(6429..6647)
                     /locus_tag="P5316_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010476276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84625.1"
                     /translation="MAGKKKAILLRIPQDLWDSLNRWSRDDLRSLNGQIEFILREAVR
                     RRAGGRTGESPRDDQVSDGPDVEKREPA"
     gene            complement(6686..7585)
                     /locus_tag="P5316_06400"
     CDS             complement(6686..7585)
                     /locus_tag="P5316_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRR84626.1"
                     /translation="MSAEYPRRTVNGWGMLGFLLVATLGLAALLVYATVEAAKAPREL
                     YASAVALLVAYVLGLGVVAILWAGFFTLQPNEGAVLLLFGAYRGTSRRDGLQWANPFY
                     KKLKISLRARNLNGDKLKVNDQRGNPIEIAAVVVWRVQDTAQAFFDVDRYENYVSVQS
                     ESAVRHLASKYPYDSSADHELSLRGSMDEVSRDLQGELQERLSKAGVVVEEARLSHLA
                     YAPEIAGAMLRRQQAEAVVAARTRIVEGAVGMVETALQQLEQHKTVELDDERKAAMVS
                     NLLVVLCGEQAAQPVVNTGTLYT"
     gene            complement(7861..8523)
                     /locus_tag="P5316_06405"
     CDS             complement(7861..8523)
                     /locus_tag="P5316_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84627.1"
                     /translation="MAFLNIIIEYEGPVVDVEPRYWAAHQAAIQAVGFHGPPRSEFWR
                     LWRTGATDSQFVRFGRPEKVADYARIRAETIDSTDLMALDQSGPKVAENLRLLKQMGS
                     CQLVSLSRNREGLNAALDRLDLWMHFESKQCLPEDRDRRVEALHKLAGGMRVTLAVVG
                     TVPMAYAAGEAGCRTVGLKNGPAVPRLLRQVGVDVFFESLDELTDALTTRHPDLQRIG
                     IF"
     gene            complement(8555..8782)
                     /locus_tag="P5316_06410"
     CDS             complement(8555..8782)
                     /locus_tag="P5316_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84628.1"
                     /translation="MKSSAGNALVLGIISLPLLLCGIGAIVGLAAVITGLLTLRDQPG
                     DRERREVTVGIFLGGLSCLVGLGAVLIELFG"
     gene            complement(8784..9176)
                     /locus_tag="P5316_06415"
     CDS             complement(8784..9176)
                     /locus_tag="P5316_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84629.1"
                     /translation="MRRDNTMNAPACGPTFRFRCLRCGSVLEARIAQVGERARCPSCD
                     AVFVVPQVDPRTGLALGGADPGHDGQYPAPVHAYAAAGHAAPRIVRLPDDSLVIECPN
                     CLHRTDIKANNCPACGRPFFPGGQRCLA"
     gene            9446..10636
                     /locus_tag="P5316_06420"
     CDS             9446..10636
                     /locus_tag="P5316_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR84630.1"
                     /translation="MSFSQAGSLFGHFLQGGVRAVITGQVGVDKKPFIERVAQIARQR
                     GKDVLVCHVGEMMYAEAPDVAPGRILDLPRSRLDLLRRSVFKDILRLAEKADNLLVNT
                     HATFRWRHGLFPAFDHDQMVELDADLYVTLVDNVDAIHERLTREHEVVHTLKDILVWR
                     EEEVLATEVMAGMIRGHGRFFVVARGIERDTAESVYRLMFEPHRRKVYPSFPMTHVTD
                     EPRILAEIDSFRDVLAEHFITFDPGDLDEKRLLIEAAAAAQRGEKSFSVESNNRRMVF
                     GVDEVNAVAADIDAQIYARDFKLIDQSDMIVSYIPELADGRPAVSSGVERELQHAYES
                     TREVYVVWRSRTDPSPFITNTATGVFSSVAELMKHFQRRGYVREYQLRLDTDQPPRHR
                     PPLG"
     gene            10644..11318
                     /gene="deoC"
                     /locus_tag="P5316_06425"
     CDS             10644..11318
                     /gene="deoC"
                     /locus_tag="P5316_06425"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRR84631.1"
                     /translation="MTTRQALAAMIDHTLLKPEATAVDIDRLCDEALRHHFAAVCVNP
                     VWVARCAERLASSTVRLASVAGFPLGASRSEVKADEARRAIDDGAVEVDMVISVGNLI
                     DGNVSSVRDDIAAVAEAVHAGSRQNVLKVILETAVLTERQIIAGCRCAAEAGADFVKT
                     STGFHPAGGATVEAVRVLRAHAGLMKVKAAGGIRTLDVALAMIAAGADRLGCSASVEI
                     VRQLPA"
     gene            11467..12852
                     /locus_tag="P5316_06430"
     CDS             11467..12852
                     /locus_tag="P5316_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84632.1"
                     /translation="MKSCLPVVLLLFHLTLALAEDKDNQGKTIAPVIDQDPAMLVPPD
                     DLKDNPAFAVAKEPPKITFSTLNGLPRRDSKTGGLWCSWGEGIVASNGKLYVGVGDHR
                     GIDANCFLYEYDPATHKHRQVLDMARLVGQKPGDYGHGKLHGRLDETPDGWIYMATYW
                     GEHPDMPPAERSKIGGRLARYNVRTDTAEDLGMPLPGDSFPMHATDAARGVFHGLGLL
                     GGYVAYDLHARRPLYAGPLPGDVTWELRSTLIDPKTGYCYGSETHTRRIVGYDPGRNV
                     FFHTKATIPLHPETGKELKPWIRSYTRQRLLDGSFICQTYDGVMFKFFPDEERTELIG
                     LNWVEGRYSTSMALSPDGRYLYYAVGAHGQTWQVGSPIIQMDTQTYARKVLAFLHPYY
                     QQKYGYVFGGSYSVSLDQKAERLLIFWNGRFRSPEEKDDSFGHPTFMCLEIPAEERRI
                     ATSPAKAQPRQ"
     gene            complement(12965..13996)
                     /locus_tag="P5316_06435"
     CDS             complement(12965..13996)
                     /locus_tag="P5316_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84633.1"
                     /translation="MPQQLNWGLISTGRISGTFARGLAHSKTGRLLAVGSRTQEAADR
                     FGKEHNVLRCYGSYEALLADKDVQAVYVSTPHPMHAEWAIKAAEAGKHILCEKPIGVN
                     HAEATAIIEAARKHDVFLMEAFMYRCHPQTHKIIELVRSGAIGEVRVVHATFSFRAPR
                     DANPRIYGKELAGGGILDVGCYCTSMSRLIAGVATGGDFAEPIELEGVGHLGPTGVDE
                     YASAVLKFPGEIIANIACGVAVQQDNHVRIFGTKGHLVVPWPWIPQREGGTSRIFLHK
                     PDDSAPQEILVRCDEWLYGMEADTVAANIERRQAPPPAMTWDDTLGNMKTLDRWRESI
                     GLVYEFERR"
     gene            14843..14914
                     /locus_tag="P5316_06440"
     tRNA            14843..14914
                     /locus_tag="P5316_06440"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14875..14877,aa:Gly,seq:gcc)
     gene            15102..15848
                     /locus_tag="P5316_06445"
     CDS             15102..15848
                     /locus_tag="P5316_06445"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR84634.1"
                     /translation="MQRYVKYFLLIGTLPLSAAIVLAAEGQKKEAGERQAKREDAAAT
                     QPAYLDKEHAPRGKGWIKLFNGKNLKGWKSIPENRPNSWKVENGILVSDFKEGEHGTN
                     IFSEKKFGDFEIYYEYLVPKNGNSGVFLRGQYEIQIQADHGVPSDKPRDWGNGGIYGQ
                     KAPSRNVSKPDGEWQSVYATMIGKKITVFLNGEKIIDNYEPPRATHLYGELKIKDGDP
                     TGPILLQGDHKPIKFRYVMIKPIGEPAEKK"
     gene            15971..16165
                     /locus_tag="P5316_06450"
     CDS             15971..16165
                     /locus_tag="P5316_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HRR84635.1"
                     /translation="MECRQKTNLKDCTCTYGGCERKGICCECLQYHLASRQLPGCCFP
                     PEAERTYDRSFKAFAKAWKL"
     gene            complement(16452..18425)
                     /locus_tag="P5316_06455"
     CDS             complement(16452..18425)
                     /locus_tag="P5316_06455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HRR84636.1"
                     /translation="MRSIAHGGLMFMCLTLLIIGRGCSPHHPADDDGSNDSFDSYVPD
                     PDVYCTPPDPDRLIRRSLPGGRTVEFAVNQMIVLMNEGTERSEVEQLAASLGGAIIGQ
                     MPDIDFYQIEVPATTQAELDALIDQVKANPVVTSAGYNGVPQFDQTCPAQSDNRDIAA
                     QDQCSFAETEYYQAVTMFDFFRQHLTLYPVRVGVIDSGVDPSTHEFDDVSILYLNDIA
                     GRPEDTHPHRHGTAVAGIIAADNDFVGVNGIASRLIGDRLRLIVGRASDDAGVIVYTG
                     LAAAAGADVINLSFGWSESDPHIETVRNIWVRLMQRHSGALFVCAAGNESTELTGLNH
                     APGGIGLSNVLTVAATAQCNPTQLSTMSNYGIAVDIAAPGENVPVVAPDGTYVGRSGT
                     SFSAPMVAALAAVLKSIDPTLTPARLKHHIAARGYPLADGSPFGRIVFTTSIQELLLD
                     MGVGDPIRSWIDPVGRHHAEASGLVLSRLCPPGMYFTIDGYGLHSVQAGGGALVGAIG
                     GPAAPPAFCINAQDGEVYLEIAGPRMGQFALGSYSIERIAIGGGVAGVSFQDHGSIDA
                     GPGVSGTLTFETCRIDQRDPWGSRHPMIVYVTGGFEGFLEVTHSDGGGPTLNEFEGGF
                     NMPLVVSPNAGDEVFEYLENNCEGGIPTSPTEP"
     gene            complement(18739..19758)
                     /gene="ltaE"
                     /locus_tag="P5316_06460"
     CDS             complement(18739..19758)
                     /gene="ltaE"
                     /locus_tag="P5316_06460"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HRR84637.1"
                     /translation="MIDLRSDTVTKPTAAMREAMAAAEVGDDVLGDDPTVIALERRVA
                     EVLGKEAALYMPSGTMTNQVAIRAHTEPSDEIIVEADAHVYYFEGGAPASLSGVMCRP
                     IQGRRGIFTAGDVRAVLRPRNVHFPPTRLICIENTHNRGGGSIWPIQDIADIAALARE
                     AGLRMHLDGARLWNASAATGIPEWEYAAHFDSVSVCFSKGLGAPVGSALAGTGEFIDR
                     ARRFRKQCGGGMRQAGIVAAGALYALEHHRRRLVDDHANAKRLAQGIADCPGIEVDPA
                     TVETNIVFVRVTAVPAAELSARLKAAGVLTNAAGDRLRLVTHLGVSREQIEQAIGAFR
                     HTMSR"
     gene            complement(20035..20847)
                     /locus_tag="P5316_06465"
     CDS             complement(20035..20847)
                     /locus_tag="P5316_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRR84638.1"
                     /translation="MSQDVSLITEDRSMIDEWDLTATNLHRVSKRCYEVAVLPIGAIE
                     AHNRHLPEGQDYRHTTHVARTASKMAWDRCQSVICLPTIPYGVDCNLLAYPLTIHVSQ
                     ATLDALVRDIIVSLRKHGIHKVLILNGHGGNEFMPLIRQLQTEIDTHVFLCNWWRVGM
                     DKYAEIFEARDDHAGEMETSVAMALYPELIERGVAGDGGVQPFRFEAMRQGWVLTSRD
                     FAKINNHCASGDPSKASAEKGRRYLDLVCGRIADFLVEVAGAEIDGKFPHVP"
     gene            20989..23679
                     /locus_tag="P5316_06470"
     CDS             20989..23679
                     /locus_tag="P5316_06470"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR84639.1"
                     /translation="MPRLTSAIVAIVVLTTPGPAALGAPPPLAPVVEYPIHPDGGIVY
                     DPLKREADRTGQLERYRSTPSNSQEIRLANCRLRVEVPRKAAAYEVIPIPYELSWEGG
                     APAFPLAVEVTAFEDESRRKGRDCHDLNLPGRIDLDVEYLGSITAHLRPNGRHNLKAD
                     MSDQPETYPPFNRRPFVRSGVLEAGDLVWFKLRYTNTGNTILDPEGLGGCLFYPQLLQ
                     KQEDGQYTAVGQAYNLYYRDLEYLYPGESHEIWLHLTSNMPGYMEGAGKSGTPQGFGI
                     SPGEYMLQLRLFARCYKTSDDFLNIWEGPEMYAWEMPFSVEAQAREAPVLPGTKTLVD
                     GGDPDKITRFIHTFEEFMSSFDCHLSAPADGTSRIGGTLHLQVAPWTSQVVAKLIATG
                     PVAIATAGIPIEIDATSLKVRFNPNPGTCMVRDGRRIPIITSQTMADMRTNVQIGPFP
                     EKHIVDRLREMMDCGINVIAPTNMPWLYDDMRNPKSNYQGDAFKYVLDVARREGMTVE
                     GWGTYPFDRANVRDIAAWITGESIPIERYTLGKSAISLTEPMLPFANSVAWLYQFHRW
                     GDLYLQVERGDVPISVEDTRGWMRQDVNVRHPMGDQTVKAFRKWVRNKYRTIEAANKA
                     WGSSFGSFDEIDPEKDRAPNRFGHRWEYTDPKHAFRDWNQAVCDLDEFRTELRVSNYR
                     DTLALVRREIPQAVICLRTEGGNVLVDGIDPTDPNPHMRHVYYSQRRCAAIAGMLQKS
                     GLIRYHSDYTTVPYTPSELRRLVRMAVEQGIIPSYFPQFDNMRDIAINERYGTEYQIH
                     YNLPEPRKGYMMHCLTALYPWFTAVHESGGIPGILWEDYQCDGFATETQKREMRLFKE
                     KLIEAMSTPEGQKALAENVRMPSQEWRKGARAMWSYKLPK"
     gene            complement(23756..24880)
                     /gene="arsB"
                     /locus_tag="P5316_06475"
     CDS             complement(23756..24880)
                     /gene="arsB"
                     /locus_tag="P5316_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRR84640.1"
                     /translation="MSSGLTKKLSFLDRYLTLWIFLAMGVGVLLGYFLPGVGEWISSL
                     RPEGTQTSIPIAIGLILMMYPPLAKVKYEELGDVFRNIKILGLSLLQNWVIGPILMFL
                     LAIVFLRGQPEFMIGLIMIGLARCIAMVIVWNDLAKGDTEYAAGLVAFNSIFQVLFYS
                     VYAWLFITVLPPLLGLQGADTSGITIGQIAESVFIYLGVPFLAGMLTRLVLVKTKGKE
                     WYHGRFIPKISPITLIALLFTIVVMFSMQGQKIITKPGDVLLIAVPLLIYFVVMFLVS
                     FWMGKKAGAGYAKSATLSFTAASNNFELAIAVAVGVFGINHGAAFAAVIGPLVEVPVL
                     ISLVNVAFYLRRRWFALVPQSAASQAADSRTCPKSDGAGW"
     gene            complement(24935..25837)
                     /gene="arsM"
                     /locus_tag="P5316_06480"
     CDS             complement(24935..25837)
                     /gene="arsM"
                     /locus_tag="P5316_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRR84641.1"
                     /translation="MSDKIIRTVQSKYAAVAGSGLSSRQDGVRQVAEAFGYSADDLAA
                     IPAEANMGLSCGNPTAFASLKPGETVVDLGCGGGLDVLLAAPKVGPTGKVIGIDMTPE
                     MIELARKNAARSAGGKMPANVEFHLATIDRLPLPDASVDCVISNCVINLAPDKRAVFR
                     EVSRVLKPGGRLAVSDIALKKQLPKAVAEDVMAYVGCIAGAILIADYEAGLRESGFAH
                     VQVIDSGADLNAYAKVENSSGCCSPTPSLPVANQPARSGCCCPKPADCGSGDLHKGMA
                     QLLSRYNINDYAASVKVYAVKPGN"
     gene            complement(25840..26112)
                     /locus_tag="P5316_06485"
     CDS             complement(25840..26112)
                     /locus_tag="P5316_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84642.1"
                     /translation="MLDDYQRSKNVTEFHGQPFDLVVTVCDNAAAACPSFPGAKCRVH
                     WPFEDPPHLPGDDEAKLVVWRRVRDQIRARVGEYLREQEKAAIQGG"
BASE COUNT         5240 a         8337 c         7800 g         4770 t
ORIGIN      
        1 caacagatcc cgaagcgacc cccgcgcgcc aaaagccgga caccgaccgc cgaccgcccc
       61 ccgcgccaaa cgaacccaac cgagatctta agtcgttatc gattaaacac ttaaggaaaa
      121 ccgtggccgg ttccccgtca tccgatccac cccgtttgtg tccctgatcg gcctgagtgc
      181 gggatactcc aacctgccct tggatccgac atcggcaatc cgctatgctt ccaccacggc
      241 cgcaagccgg aagcggccgc cgagcaactg ccaccatgga accgaatcaa cagcggtgca
      301 acagcgatga agttcacgaa actgcacggc ctgggcaact gctacatcta cgtcaactgc
      361 ttcgacgagc gagtgaacga cgctccctcg cttgctcggg cggtcagcga tcgtaacacc
      421 ggcgtcggca gcgacggcct gatcctcatc tgcccctcaa gaacggccga cgcgcgaatg
      481 gagatgtaca acgtggacgg ctcccgcggc cagatgtgcg gcaacggcat ccgatgtctg
      541 gccaagtacg tctacgacca cggcctctcg cgaaacaacc cgctgcgaat cgagaccgac
      601 gcgggaatca agaccgtcag cctgaacatc gaagacggca aggtcgcttc cgcctgcgtc
      661 gatatgggcg aacctcggct gcgtcccgag gatttgcccg tcaagctgac ccaggaacag
      721 atgatcgacc acccgctcga gatcctcgac catcaggcgc tcatgacctg cgtctcgatg
      781 gggaatccgc atgcggtgat ttacggggtc cctctcaagc tgctgaacaa tcggggaccg
      841 gccgggtgga acctggaaat cgagggccgc cgtatcgaga ccgccgccat cttccccgaa
      901 cgtacgaacg ttcatttcgt ggacgtgctc cacccgagcg aggtgaaaat gctcacctgg
      961 gaacggggca gcggggcaac ccaggcctgc ggaaccggcg cctcggccgt atgcgtggcc
     1021 ggagtcctga ccaaacgcag cagccgcaag atcattgctc atctccccgg aggcgatctg
     1081 accctcgaat ggcgcgaagc ggacaaccac gtctacatga ctggtccggc agtcgagatc
     1141 ttcacgggcg actggccggc gtgaccggca ggactggccg cccagccgag ccgaaaggtt
     1201 aacgggagac ggtgccgcta tcgagaactc cgccggcgtc cgttgagatc gtcccttttt
     1261 ccacgagttg accgactgtc agccgaccgc tgttccggcg atcgtgcaga atctgcagtg
     1321 ccaggacgtc gtagtatgtc atcatcggcg agaaatcgtt caggtagctg ctgtccttcg
     1381 gcgggggtgt gatcccctgc atacgcgaga gcagcccgac gcgagcgtgg cacaatccca
     1441 gcgtgtgccc gatctcatga gccagcacgc gcggacacgt ctgccgcgga tcctgcccga
     1501 cggccagcac cacctccatc ggcggcatga acaaaccgcc ggcatcccat ttcatgaagg
     1561 tcgcgccgcc gcggcccacc agtgcgggag aatcggccgt gacataccga atgggcagaa
     1621 ccaccgcatt ggccggtggg cgtcgcatct ggccgatgcc gagctccgat cgcagggacg
     1681 cccccgtcca gtcattgatg cggtccacaa ggaatcggac gtaacgctcc actgcggagt
     1741 tgggcgggtt gaccacattg aagatcagcc gcgtgtccgc ttcccatcgc ctgatcagcg
     1801 attccgggcg gatcggccct cccttgtcgg acaatgggcc cgtgtctccc agtagggcgc
     1861 tgagaatggg accacaatcg gccccggcaa cgacgttgaa ctcgctcgtc gattccgcgg
     1921 acgtggctgc ggcagtaatg acatggccgg ccggaacgtc gcggcagtct actgtgaagc
     1981 gtcccatgcc atccgcgctg accgtcctga ggaatcgagc cgcctcgccc acccgatcgg
     2041 cgtaaagctc gaccgtggca ttcggttcgg ccgtgcacgt gacctgcggg ccgtcgatgc
     2101 gaacgggcgt cggtgccagc gggctgatat gcagcggtcc gcggcagatc ggccgcatcc
     2161 cgttccccga gaaaatgcac cggctggccc gaaccggccc gccttcgtag gcgtcgggac
     2221 aagcaatccc ggatcgccgg ttgcctgtga aaacgcagcg gtcgaaccat ccgcgggcct
     2281 gctggcgcag acaaaccccg tgagagccgc tatccgtgat gcgcacttgt gatgcgtgga
     2341 cggacgactt cccgaaaagc accatgccgt attccccgcc aaactccatc gcggaagctt
     2401 ctatcgtggc ggcggcttca tcctgaagct cgatgcaccg gctgctgtgg cgacgcagcc
     2461 tcgtcgaggt caggtcgatc acggcctgac cggtcgcgaa gatgcctgtt cgctcgctct
     2521 cgtcgatggt tgaatccgca atccgaagct tggccctttc gctggcgtcg gcattggcga
     2581 agcgggcccg cgacagcgcg cagcgcgtca gaatggcctg ggcctcgtcg ctgagaacga
     2641 cgttccagtc ggccgcactc gacatctggc agccggcggc gaagattcct ccctgatcaa
     2701 agccggcgac gtcggattgg ccgttgccgc tgagtcttac atcctcaaga tcggccaagg
     2761 cacgatcgcg cacctccacg ccgtgactgc cgttcgtgtt gatcgacccg ccccgaatgt
     2821 gaactctggc ttcgccgaaa accgccacac ccggccccag acagtcgcga accagcacgt
     2881 tgtccagatt gagcctggca tttgcagaaa tcaggattcc tcgaaggcgg cccccgcgaa
     2941 cggcgagatt catcaggctg atctgcgcct tgccggaaac catgatgccg gcgtccgggc
     3001 agtcagagct gtccagcgtc accagaccgt cgggcaatgc gccgtcgatt cggtcatggc
     3061 cgcccgattc gtcactgatc acgagcgcct caggcagccg gatggaaagc tccgaagact
     3121 tgaagacctt gggatcgaat tggatcacgt tgtcatgggg atcttggcgc gcttcgatca
     3181 acgcgtcaat gagagtcaac tgcgcagtgt cggctgatct tccaggatgc gggcttgaaa
     3241 cgacgagcgg acgcaaccgg cccgttcccg cggatgcttc cggatgctga tcgcgcgtgg
     3301 ccacctgaac gccgacgaat gcccccacga ccagaatggt cgtcagcagg ggtggtcggc
     3361 tcttgcggcg cgaacgggtc gacgaacgca tcggacagaa atccttattc cgtgtcggta
     3421 ctgagagcgg tgccggccga tcggttgggg aacactctgt gtagagcgtg gtcccggagt
     3481 gtccgccgtc cccgcccctc cgtggtcccc tggcgggacg tatccaagcc agtcccgagg
     3541 acgtatcgac ttgtgagaag acgcgggtta gtagtggaaa agcccccacc tcaagcactg
     3601 aaggaatcag ccgtccgacg cgtcccataa ccccgctcgg cattggccgg cgacctgccc
     3661 ccgcgagttc ccggtcgaca aaacagagaa cctgcgaatc gcgttgagta ccaggatgcg
     3721 gcgttgtatc ctgcacgcat gaccagagtc ctcttgctga ccgtggacga ggggttggcc
     3781 gacgtcgtca ggggaatctg ccggcagcgt tcgttcgagt atgttgtcct gcccgggctg
     3841 ccctccgcga agaccccgaa agactacttc gacttggttc tcgttgatgc ccgaccggca
     3901 ccgaccagca ccctgtcgtt caacaccatc ccacccatca ctcttgagaa gactgctccc
     3961 ttggaagccg gacgggttct cgcgataagc cactatggaa cgctcgtggg acggatgacg
     4021 ggctacgtca gcggagccag cggctactgc cgaatccatc cgttcggcat ttcttgcgat
     4081 cctgcgctct ccgagcagct cacggaggcg attcttgacg gcctttcgct tcccgaatac
     4141 cgacgcgtcg atttccatga cgcttttcct gatcgggact tctaccgcca ggtcttcgaa
     4201 agcattcaaa tccccatcgt cctcatcacg agcgagggga tcatcaaact catgaacctc
     4261 cgcgccctgg cacgattcaa ctacttcacc cagatggtca tcaaccagtc ctggctgatg
     4321 cttgtcgccc cgcaagaccg cggcaaacgg gccgaagact ttctggtgcg catcagcggc
     4381 ggctatttct acgaaggaac gttgcgctgc gtcgaccgcg ccggcaagga gtttccggga
     4441 ctgatcacca gcagccgcgt gttcgccccg acgcacgggt cggacatgct gatcatcctt
     4501 gcgatccacg acctgaccga aatggaggag ctccagagac aggttacgac ttttcagaag
     4561 atggagtcga tggagcgagt ggtcgcgggc ctgacccacg agttcaacaa catgctgacg
     4621 gccatcctgg gtcacgccga aatgctgaca caggacctgc ctcgtgggac cgatctctac
     4681 gacagcgctc gcgtcatctg ccaggaagcg gaacgcgccc gcgagatgac cgcgcggctt
     4741 ctcgggctgt cgagcagccg gcagtttgtt cccggccgcg tgtcctgcaa tcaggtggtc
     4801 cgcgaaaccc aggccctcct gcgtcactct cttggcgacg cggtcagaat cgaaacctgt
     4861 ctgatggaat cgggcgatgc cgtcgacggg gacgccagtc agctccggca ggtactcgtg
     4921 aacctgtgtc tcaatgctcg cgacgcgatg aatggcaaag gcgtgatcac catccgcacc
     4981 tccttgcgcg aggtgactcc cgaagaatgc ggtccgcatc aagactggac cccgggaatg
     5041 tttgttgaga tctgcgtgca ggaccagggg tgcggcatcg cccccgatat catcgggcgc
     5101 gtgtttgagc ctttcttcac gaccaaacag gacggggccg gaacgggttt ggggctctcg
     5161 gttgtgcggg gcatcgtgcg aacccatggg gggcacgtga ccattgaatc aaaggtcgga
     5221 cagggaacct cggtgagggt ccggttgcca cagagagagg cccaggccga ggaagctgat
     5281 cgtgcggacg agaacccgcc ggccgccgtg ccgtgtggta agggagcttg cgtattgatc
     5341 gtggacgacg accgggcggt tctgacctat gcgcagaaag ctcttcagcg ggccggctac
     5401 aaggtgagga tggcgacgca cggtttgctg gcactagaga tattcgagca ctaccgggat
     5461 gagatcgagt tgattatgct cgatctgaca atgccgggaa ccgccggccg cgacatactc
     5521 aagagcctcc ggaagatgga cgccaaggta ccaattgtga tatgcaccgg tttcgcacac
     5581 ggtggcatgg acgacgattt gctcagccag gtgcagggct tcattaagaa gccgtttcga
     5641 ccgaatgatc tgatcgaaca ggttgccgaa gtgctccagg cggccaggga ggagtaattc
     5701 accccacagc cgaggatggg cgacatcggc gccattcagt cgcgccgtga catgccgttc
     5761 tggcatggcg cgacatcaag gtcgtgcgaa aaatcgcgcc aaaatggtgc ggtaggcatg
     5821 cttcatcagc ccatgcgagg tcgtccgttg cgtgttgaaa aatctctctg gaccggcgta
     5881 taatcgagcg aggagggagg gcagagattc aggttgttga ataatcccgg cggtttggct
     5941 cgtcagaggg tattgggggc cgacgaaaag ggccgctttt gtggaaagtg gtgagagcca
     6001 tgtcagagca ggacaagaag caatcgggag gcgacgaagg cacctgcaat gcggtgcctg
     6061 tgaaacctcg aagagagcga ctaccccggc agtcgccgcc tcgggttctt cccccgtgga
     6121 aggtgatcct tcacaacgac gatgtcaacg agatgaacag ggttgtggag gttatctgtc
     6181 tgttgacccg attgaaccgg caggaagccg ccgcacgcat ggaggaggcc cacaagaccg
     6241 gcgcggcgct gttactggtt acccaccggg agcgagctga gctctatgta gaacaattcg
     6301 ccacctacaa ccttacggtc acggcggagc ccgaagtcgg ctgacgtctt cctgatattc
     6361 tgttcatgag tcttccaggc ggcttgcctc ctgcgcgtgg atgagtcgcc gaaaaaggca
     6421 cagctcattc acgccggttc acgcttctcg acatccggcc catccgagac ctggtcgtcc
     6481 cgggggcttt ctcccgtgcg tcctcccgcc ctgcggcgga cggcctctcg cagaatgaat
     6541 tcgatctgac cgttcaggct gcggaggtca tcacgcgacc aacgattcag actgtcccac
     6601 aaatcttgcg ggattcggag caggatggct ttcttcttgc cggccatgag tgactgtcaa
     6661 tctgaagccc aaacccggtt ttcgatcagg tgtagagcgt acccgtgttc acgacgggct
     6721 gcgccgcctg ctcgccacac agcactacca gcaggttgct gaccatcgcg gccttgcgct
     6781 cgtcgtccag ttcaacggtc ttgtgctgct cgagctgctg cagcgccgtc tccaccatgc
     6841 ccacggcacc ctcaacgatc ctcgttcgcg cggccacaac cgcctccgcc tgctgccgcc
     6901 gcagcatggc ccccgcaatc tccggggcat acgccagatg gctcaggcga gcttcctcaa
     6961 cgaccacacc agccttcgac agcctctcct ggagctcccc ctgcagatca cgagaaacct
     7021 cgtccatgct gccgcgcagc gacagttcgt gatcggccga tgaatcatac gggtacttac
     7081 tggccaggtg ccggaccgcc gattcgctct ggactgatac gtagttctca taccgatcga
     7141 cgtcaaaaaa ggcttgggcg gtgtcctgaa cgcgccagac cacgaccgcg gcgatctcga
     7201 tgggattgcc gcgttgatcg ttgactttca gcttatcgcc gttgaggttt cgagctctca
     7261 gcgagatctt gagtttctta tagaacgggt tggcccactg caacccatct cggcgcgatg
     7321 tgccccggta tgccccaaaa agcagaagca ctgcaccttc gttcggctgc aacgtgaaga
     7381 aaccggccca gagaatcgcg acgacaccca ggccgagcac atacgcgacg agcaacgcga
     7441 cggccgaggc atagagctcc cgtggtgctt tcgcggcctc gacggtcgcg tagaccaaga
     7501 gtgcggccag ccccagcgtg gcaacaagca ggaatccgag catcccccat ccattgaccg
     7561 ttcgacgcgg gtattcggca gacatggttg tttcctcccg ggccaatggc cctatttgat
     7621 atcattaata tatcagaaaa atatcgaaat gcaagcaaga aatgcgagaa acgtaaactt
     7681 ttgaaaaaac aaagtttacg attatcacca ttaggaacca gcgcgagttc cggctctccc
     7741 gggaaacggg accaatgctt caaagcccca gtctcgccgc gcagcttcgc tgacttaatc
     7801 gggtgccaag taaggaaaag acccaagcgc gatcaacccc gactgcgatc agagcttgcc
     7861 tcagaaaatg ccgattctct gcaggtccgg gtgacgggtg gtcagagcgt cggtcagttc
     7921 gtcgagcgat tcgaagaaca cgtccacgcc cacctgacga agcagtctgg gcacggccgg
     7981 cccgttcttc aacccgaccg ttcggcagcc ggcctcacct gcggcataag ccatcggcac
     8041 tgtgccgacg accgcgagcg tcacgcgcat gccgccggcc agcttgtgga gggcctcgac
     8101 gcgccggtcg cggtcctcgg gcaggcactg cttgctctca aagtgcatcc agagatcgag
     8161 gcggtcgagc gcagcgttca ggccctctcg gttgcggctg agcgaaacga gctgacaaga
     8221 gcccatctgc ttgagcagtc gcaggttctc agcaaccttg ggtccggatt ggtccagcgc
     8281 catcaaatcg gtgctgtcga ttgtctccgc ccggatgcgg gcgtagtcgg cgaccttctc
     8341 cggccggccg aagcgaacaa actgcgagtc ggtcgccccg gtccgccaca gcctccagaa
     8401 ctccgaccgc ggcggcccgt gaaaaccgac cgcctgaatc gccgcctgat gcgccgccca
     8461 gtaacgcggc tcgacatcca cgacgggccc ctcatactcg atgataatgt tgagaaaagc
     8521 catatgcgct tagccacagg actccaccat catctcagcc gaacaactcg atcagtaccg
     8581 ctcccagtcc gaccaagcac gacaacccgc ccagaaagat gcccaccgtc acttctcgcc
     8641 tctctcgatc gccgggctgg tcccgaagcg tcaacaggcc ggtgatgacc gccgccaaac
     8701 ccacaatcgc cccaattcca cacagcagca gcggcaacga gatgataccc aggaccaggg
     8761 cgttgccggc actcgatttc aagtcaggcg aggcagcgct gccctccagg gaaaaacggc
     8821 cgtccgcagg ccggacagtt gttggccttg atatctgtcc gatgtaggca attcgggcat
     8881 tcgatcacca ggctgtcgtc gggaaggcgg acgattcgcg gagcggcatg cccggcggcg
     8941 gcgtaggcgt gtaccggtgc ggggtactgg ccgtcatgac cggggtccgc gcctcccaac
     9001 gccagtccgg ttcgaggatc cacctgaggg acaacaaaga ccgcgtcaca cgaagggcag
     9061 cgagcccgct cgccgacctg agcgatgcga gcctccaaca ccgacccaca ccgcaggcat
     9121 cgaaagcgga aggtcggtcc gcaagccggg gcgttcatgg tattgtcgcg tctcacggac
     9181 ctcatggtac tgagattgcg cggtaaggaa acaggcgttg gccctgttgt ggtgccgacg
     9241 tccccgtcgc tggtgttgtg tgaacctgtc ctgccaatcg gctcggcttg gggaggagtg
     9301 tatcgttctg gcccggatgg gccaacgagg gcgttggccc cgctgtggtg ccgacgcccc
     9361 cgtcggcaca tccggtcgca cgggcggttt tgaccctggt ggttcgggca gatagcatgc
     9421 acccggtttg attcgggagc gtgtgatgag tttcagccaa gccggctcgt tgtttggtca
     9481 tttcctgcaa ggaggagtgc gggcggtcat taccggtcag gtgggcgtgg acaaaaagcc
     9541 gtttatcgag cgggttgccc agatcgccag gcagcgaggc aaggatgtgc tggtctgcca
     9601 cgttggcgag atgatgtacg ccgaggcccc cgatgtcgcc cccggaagaa tcctcgatct
     9661 gccccgttca cggctggacc tgctacgccg gtcggtcttc aaggacatcc ttcggctggc
     9721 cgagaaggcc gacaacctgc tggtcaacac tcacgccacc ttccgctggc gccatggcct
     9781 ttttccggcc ttcgatcacg accagatggt cgaactcgac gccgatctgt acgtaacgct
     9841 ggtggacaat gtcgatgcga tccacgagcg cctgacccgc gagcacgaag tggtacacac
     9901 tctcaaggac atcctcgtct ggcgcgagga ggaagtgttg gcgaccgagg tgatggccgg
     9961 catgattcgc gggcacgggc ggttcttcgt cgttgccagg ggaatcgagc gggacacggc
    10021 cgagagcgtg taccgcctga tgttcgaacc gcatcggcgc aaagtgtatc cctcgtttcc
    10081 aatgacccac gtgaccgacg agccacgaat ccttgctgaa atcgattcct tccgggacgt
    10141 cctggccgag cacttcatca cctttgatcc gggtgacctg gacgagaaac ggctgctcat
    10201 cgaggcggcg gcggcggcgc aacgcgggga gaagagcttc tccgtcgagt ccaacaaccg
    10261 gcgaatggta ttcggcgtcg atgaagtcaa cgcagtggcc gctgacatcg acgcacagat
    10321 atacgctcgc gatttcaagc tcatcgacca gtcggatatg attgtcagct acatacctga
    10381 gcttgccgac gggcgacccg ctgtttccag cggcgtcgag cgggagctgc aacacgccta
    10441 tgagtcgacg cgcgaggtct acgtcgtctg gcggtcacgg accgacccgt cgccgttcat
    10501 caccaatacc gcgacggggg tgttctcatc ggtcgcggag cttatgaagc acttccagcg
    10561 cagaggatac gtcagagaat accagttgcg cctcgacacc gaccagcctc ctcgccaccg
    10621 gcctcctttg ggatgaatcc gcgatgacca cccgacaggc attggccgcg atgatcgatc
    10681 acacgctgct caagcccgag gcgacggccg ttgatatcga tcgactctgc gatgaggcct
    10741 tgcgacacca tttcgcggcc gtgtgtgtca atcccgtttg ggtggcccgc tgtgccgagc
    10801 ggctcgcatc gtcgacggtc cggctggcga gtgtggcggg ctttccgctg ggggcaagcc
    10861 gttccgaggt gaaggccgac gaggcccggc gggcgatcga tgatggagcc gtcgaggtcg
    10921 atatggtcat cagcgtcggc aatctgatcg acggcaacgt gtcttcggtg cgcgacgata
    10981 tcgccgctgt ggcagaggcg gttcacgccg gctcgcgtca gaacgttctc aaggtgatcc
    11041 tggaaacggc cgtcctgacc gaacggcaga tcatcgccgg ttgccgctgt gccgccgagg
    11101 ctggagccga tttcgtgaag acgtcgaccg gcttccaccc ggcaggggga gccacggtgg
    11161 aggcagttcg cgtgcttcgc gctcacgccg gattgatgaa ggtcaaagcc gctggcggca
    11221 ttcgcacgct ggatgtagcc ttggccatga ttgccgccgg tgccgatcgc ctgggctgct
    11281 cggcgagcgt ggagattgtg cggcagttgc cggcataaag accggcctct tgacgaaccg
    11341 gccgattcat cccgccggtg cgttcggtat gcgatgaaga ctttgggaca actgtcgccc
    11401 gtctggcgat actctcccga ggtcgacgac ggttgcggtg gatactcata gcgaggtgcg
    11461 agtgaaatga agagctgttt gccggttgtc ttattgttgt ttcacctgac gctcgcgtta
    11521 gcagaggaca aggacaacca ggggaagacg attgcccccg ttattgatca ggacccggcg
    11581 atgttggttc cgccggacga cctgaaggac aacccggcgt ttgctgtggc caaggagccg
    11641 ccgaagatca ccttcagcac gctcaacggt ctgccgcggc gcgacagcaa gaccggcggg
    11701 ttgtggtgca gttggggcga ggggattgtg gcctccaacg gcaagctcta cgtcggcgtg
    11761 ggcgaccacc gggggatcga cgccaactgc tttctgtacg agtacgaccc cgccacccac
    11821 aagcaccggc aggtgctgga catggcgagg ctcgtcggcc agaagccggg cgactacggg
    11881 catggcaagc ttcatggccg tctcgacgaa acgcccgacg gctggatcta catggccacc
    11941 tactggggcg agcacccaga catgccccct gccgagcggt cgaaaatcgg tggacgactg
    12001 gcccgctaca acgttcgtac cgacacggcg gaggacctgg gcatgccgct gcccggcgac
    12061 agcttcccga tgcacgccac cgacgcggcc cgaggggttt ttcacggcct tgggttgctc
    12121 ggcggatacg tcgcctacga tctgcatgcc cgccgtccgc tctacgccgg ccccttgccc
    12181 ggcgatgtta cctgggaact gcggagcacg ctgatcgacc cgaaaaccgg ctattgctac
    12241 ggcagtgaga cccacacccg ccggatcgtc ggctacgatc ccggccgcaa cgttttcttc
    12301 cacaccaagg cgacgatccc gctgcatccg gagacaggga aagagcttaa accctggatc
    12361 cgctcgtaca cgcgccaacg gctgctcgac ggctcgttca tctgtcagac ctacgacggg
    12421 gtgatgttca agttctttcc tgacgaggaa cgaacggaac tgatcggact gaactgggtc
    12481 gaaggccgat actcgacctc gatggccctg agcccggatg gccggtacct ctattacgcc
    12541 gtcggggccc acggtcagac ctggcaggtc ggctcgccga tcatccagat ggatacgcag
    12601 acgtatgctc gcaaggtgct ggccttcctg cacccgtatt accagcagaa gtacggctac
    12661 gtgttcggcg ggtcgtactc ggtcagcctg gaccagaaag ccgagagact gttgatcttc
    12721 tggaacggtc gttttcgcag cccggaggaa aaggacgact ccttcggtca ccccactttc
    12781 atgtgcctgg agattccggc tgaagaacgc cggatcgcga cctcgccggc gaaagcacaa
    12841 ccgcgacagt gagcggaatg acagcgaggc cgattcaagt ggcggggcat gacgcctcgc
    12901 agcactttcg aggcaggtcc tcgtcgccta tcgcgacttc gatcgggtct gaagcttgtg
    12961 ccagtcaacg tctctcgaac tcgtagacca acccgatcga ttcacgccag cgatcgaggg
    13021 tcttcatgtt gcccagggtg tcgtcccaag tcatggccgg cggtggcgcc tgccgtcgct
    13081 caatgttggc agccaccgta tcggcctcca tcccatacag ccattcgtcg caccggacga
    13141 gtatctcctg cggggcgctg tcatcgggct tatgcagaaa aatgcggctg gttccgcctt
    13201 ctcgctgagg tatccagggc caaggcacaa ccaggtggcc tttcgtaccg aagatgcgaa
    13261 cgtggttatc ctgctgaacc gcgaccccgc aagcgatgtt cgcgatgatc tcgcccggaa
    13321 atttcagcac cgccgaggcg tactcgtcaa cgcccgtagg gcccagatgg cctacgccct
    13381 caagttcaat gggctcagcg aaatccccgc ccgtcgcgac gcccgcgatc agtcgggaca
    13441 tcgaggtgca gtaacagccg acgtccagaa ttcctccacc ggcaagctcc ttgccgtaga
    13501 tacgagggtt cgcatcgcga ggcgctcgaa agctgaacgt tgcgtggacc actcgaacct
    13561 cgccgatggc tccgctccga accagttcga tgatcttgtg cgtctgcgga tggcagcggt
    13621 acatgaacgc ttccatgagg aagacgtcgt gcttgcgggc ggcttcgatg atcgccgtcg
    13681 cctcggcatg attgacgccg atgggtttct cacaaaggat gtgcttgccg gcctcggcgg
    13741 ccttgatcgc ccattcggcg tgcatcgggt gcggcgtcga gacataaacc gcctgcacgt
    13801 ctttgtcggc caacagagct tcgtagctgc cgtagcaccg caggacgttg tgctccttgc
    13861 caaacctgtc cgcggcctcc tgcgtgcggc tgcccaccgc cagaagcctc cccgtcttcg
    13921 aatgggccag cccacgggca aaggtcccgg aaatccgccc tgtgctgatg agcccccagt
    13981 tgagctgttg tggcatcgga tgatcctcgc gattccatac cccgaccttc tatgttcccc
    14041 gttcaacatc tcatatacac attccgcgct cctgaacttg ccaagacccc atgccggaat
    14101 cttcacattg ccggcctcgt tcattcggtg cccacttggg ggctccttcg ggatcagcgg
    14161 ctcatggccg ggactatgat ccggcagaaa ggccgtgcgg gaaacaggcc tcttatagtc
    14221 tcttgccggt tcatagcccc ctcgcgcaac caacccgacg ttaagatggg caacctccgc
    14281 tcggaaccgc cgttaccgtt gcccgcccca cccggcaaac cttgcaggat gaaccaacgc
    14341 attggggctc atgagttctc gtctcattcg cgtggcgcca aatcctgccg cgctgcccgg
    14401 cggatcccga catccgacgt tttggccgtt ggaggtgagt ccctggccat tcatccgccc
    14461 ataccatgaa gacttaaata tctgcacata aagaacatac ggatcatcca gcctgcttct
    14521 cgggccgtcc cggctcgctg ggtggccgaa aaccctggtt ggggcgtccg gtcggggatg
    14581 ctgggaacca accagccgcc tagctgcgat tgggccaaag cggcggccgc gcctcccctc
    14641 ggattcgtga cacaattggc ggaggttctg tcccaggccg caggcaactt gtccgatcgc
    14701 gcaagcacgg ccaacaggta agcctcgcca agagcctgcc cgtggcaccc tcgcttggat
    14761 tgtcgcgcac ccaagcgccc gtgtatccgc aaacggcagg tgttgaaaag cctgcgacgg
    14821 cgcgtatcat gagagccgga acgcgggtgt agctcagtgg tagagcgtca cgttgccaac
    14881 gtgaatgtcg tgggttcaag tcccatcacc cgcttttgag tgccatgggg gtggttgggg
    14941 caagtccgca catttccgca cgccggccgg ttggccgtcc accaaggtcg ggagtcccct
    15001 gttttcaacg ccggcgacgg agtatcatga tcggcccgtt ggccggacga cctgttgcgg
    15061 agactggccg gctcggcttg ggatgctaac gaggtgtcac catgcaacga tatgtgaagt
    15121 acttcctgtt gatcggcacc ctgccactat cggcggcgat cgtcctggcg gcagagggcc
    15181 agaagaagga agccggcgag cggcaagcca agcgcgagga cgccgcggcg acccagccgg
    15241 cctatcttga caaggagcat gccccgcgag gaaagggctg gatcaagctc ttcaacggaa
    15301 agaacctcaa gggctggaaa tcgatccccg aaaaccgccc caactcttgg aaggtggaga
    15361 acggtattct cgtcagcgac ttcaaggagg gcgaacacgg gaccaacatc ttcagcgaga
    15421 agaagttcgg cgacttcgag atttattacg agtacctcgt gcccaagaac ggcaacagcg
    15481 gggtctttct ccgcggccag tatgagatcc agattcaggc ggatcatggc gtgccgtcgg
    15541 acaaacccag ggattggggc aacggcggaa tttacggcca gaaagctccg tccaggaacg
    15601 tcagcaaacc ggacggcgag tggcagagcg tctacgccac gatgatcggc aagaagatca
    15661 cggtcttctt gaacggggag aagatcatcg acaactatga gccccccagg gcgacgcacc
    15721 tgtacggcga actcaaaatc aaggatggcg acccgaccgg cccgatcctg ctccagggcg
    15781 accacaagcc catcaaattc cggtacgtga tgatcaagcc catcggtgag ccggcggaga
    15841 agaagtagtc tgcaagacgt ttggcaatac gagatggacg cgtcgccgga caaacacgcg
    15901 tccatcccga gtgcgtcctg ttcgcgatca cgcctacccg gacgagatga cgagcggagg
    15961 agaatcgatc atggagtgtc gacagaaaac gaatctcaag gactgcacgt gcacctatgg
    16021 cggttgcgag cgcaagggta tctgctgcga atgcctgcaa taccacctgg catcccgcca
    16081 actgcccggt tgctgcttcc cgcccgaggc cgagcgcacg tatgaccgca gcttcaaggc
    16141 cttcgccaag gcctggaagc tttagtctct tgcgtcgcga ctggtacgca ttctgcgcgc
    16201 atgtttctgg atcgaagagc gtttgggttg cggcccggaa aaaggcggtc cgccttagga
    16261 agtcttgcgt tccaacggtt tgcggcgttc ggatggccct gcagaccctg ccgtccgaag
    16321 ctgcctcgct gagcattgac cggcaagccg cccggggcat ccggcgttcg caggagagcg
    16381 cgacccgctc ccgctccccc cttgtagagt aggagagtct tcgcgggtga tccgccggtt
    16441 gcatcgaccg ctcacggttc ggtcggcgag gtcgggatgc cgccctcgca gttgttctcc
    16501 aggtactcga atacctcgtc gccggcgttc gggctcacaa cgagcggcat attgaaacct
    16561 ccctcgaact cgttgagcgt gggaccaccg ccgtccgagt gcgtcacctc taaaaaacct
    16621 tcaaaacctc ctgtaacata cacaatcatg ggatgcctgc ttccccaggg gtcgcgttgg
    16681 tcgatacgac acgtctcaaa agtgagcgtc cctgaaactc ccgggccggc atcaatggaa
    16741 ccgtgatcct gaaagctgac cccggcaacg ccgccaccga ttgcgatcct ctcgatgctg
    16801 tacgagccga gggcaaactg gcccatgcgc gggccggcga tttcgaggta cacctcaccg
    16861 tcttgcgcgt tgatacagaa cgcgggcggc gccgccgggc cgccgatcgc tccaaccagc
    16921 gctccgccgc cggcctgaac ggaatgcaat ccgtatccgt caatggtaaa gtacatgccg
    16981 ggcgggcaga gccgactgag caccagtccc gacgcctcgg cgtggtgacg cccaaccgga
    17041 tcaatccacg agcgaatcgg atcgcccacg cccatgtcca gcaacaactc ctgaatgctc
    17101 gtggtgaaaa cgattcttcc aaacgggctg ccgtcggcca gcggataccc acgcgccgcg
    17161 atgtgatgct tgagccgggc cggcgtcagg gtcgggtcga tcgacttgag cacggcggcc
    17221 agcgcggcaa ccatgggcgc cgagaaggat gttccggaac gaccgacgta cgtcccgtcg
    17281 ggagccacga cgggcacatt ctcgcccggc gccgcaatgt cgacagcgat gccgtagttg
    17341 ctcatcgttg agagctgggt cggattgcac tgcgccgttg cggccacagt caagacattc
    17401 gacaacccga tgccgcctgg agcgtggttc aggccggtca actccgtgga ttcgttccct
    17461 gccgcgcaaa cgaataacgc gccgctgtgc cgttgcatca gcctgaccca gatgttccgc
    17521 acggtctcga tgtgcgggtc agactccgac caaccgaagc tcagattgat gacgtcggca
    17581 cctgcggccg cagccaagcc cgtgtaaacg ataacaccgg catcgtcgct cgcgcggccg
    17641 acgataagac gaagacggtc tccgataagc cgtgatgcga tgccgttgac accaacgaaa
    17701 tcgttgtcgg ccgcgatgat ccccgctact gctgtgccgt gtcggtgggg gtgcgtgtct
    17761 tcgggccttc cggcgatgtc attcaggtag agaatgctca catcatcgaa ctcatgcgtg
    17821 gagggatcaa cacccgagtc aatgacgccc accctgaccg ggtatagcgt caggtgctga
    17881 cggaaaaagt cgaacatcgt gaccgcctgg tagtattcag tctcggcgaa cgaacactgg
    17941 tcttgagcag cgatgtcccg gttatcgctc tgggccgggc aggtttggtc gaattgggga
    18001 acaccattgt aaccggccga tgtcacgacg gggttggctt tcacctggtc gatgagggca
    18061 tcaagctcgg cctgtgtcgt cgccggcacc tcgatctggt agaaatcgat gtccggcatc
    18121 tggccgatga tggcccctcc cagcgaggcc gcaagctgct cgacctcgct gcgttccgta
    18181 ccttcgttca tcaaaacgat catctgattg acggcgaact cgacggttcg gccaccgggc
    18241 aggctccgcc ggatcaaacg gtccggatcg ggaggggtgc agtagacgtc cggatcggga
    18301 acatatgagt cgaaggaatc gttagaacca tcgtcatcgg caggatgatg cggcgagcac
    18361 cctctgccga tgataagcag cgtaagacac atgaacatca ggccgccatg tgcaatgctt
    18421 ctcatcacgt tctctccggc aagtgggacg agaacaaccg cacggctgcg cggcatgagg
    18481 gcggattacg gcacgacaag ttgtgcccgg atcaccacac gcagaaccgt ctttcgacct
    18541 gtcctccacc cgtcaacgcc ttgaacagat actttctcgc tccattgtat cagaaacaca
    18601 ggcatattca aggatcaaat ggggaacaaa aagataatac gggcaacagt gggccgccct
    18661 gacgggtgca tgggtcgcgc ctccgcagcg atcactcaat ggcaggagtc aggttgacac
    18721 cggattggag gcggatcgct atcttgacat ggtgtgtcgg aacgcgccta tagcctgctc
    18781 gatctgctct cgcgacacac caagatgggt aaccagccgc agtcggtcgc cggcggcgtt
    18841 ggtcagtacg ccggcagcct tgagtcgcgc ggacaactct gcggccggca cggcggtgac
    18901 cctcacgaaa acaatgttcg tctcgaccgt cgccggatcg acctcgatac ctggacagtc
    18961 cgcgatgccc tgtgcaagcc tcttagcgtt ggcgtgatcg tcgacgagac gccgccgatg
    19021 gtgctccagc gcatacagcg cccctgctgc gacgattccg gcttgccgca tgccgccgcc
    19081 gcattgcttg cgaaagcgcc tggcccggtc gatgaactcg cctgtgccgg ccagggccga
    19141 gcccacggga gccccgagac ccttcgagaa gcagacgctc accgagtcga aatgggcggc
    19201 gtattcccac tcgggaatcc ccgtagccgc cgaggcgttc cataaccgag ccccgtccag
    19261 gtgcatccgc aaaccggcct cccgcgccaa ggcggcgatg tcggcgatat cttgaatcgg
    19321 ccagatgctc cccccgccgc ggttgtgcgt gttttcgata cagatcagcc gcgtcggcgg
    19381 aaagtgaacg tttcgcggcc tcaacacggc ccgcacatca ccagcggtaa agatgccccg
    19441 ccgcccttgg atcggccggc acatcactcc cgacaacgac gccggcgcgc cgccctcaaa
    19501 gtaatatacg tgcgcgtccg cctccacgat gatctcgtcg ctgggctccg tgtgagcacg
    19561 aatcgccacc tggttggtca tcgtgccgga gggcatgtag agggccgcct ccttacccag
    19621 aacctcggcc acccgccgct ccagggcaat taccgtcggg tcatcgccca acacatcatc
    19681 accaacctcc gccgcagcca tcgcttcgcg catggcggcc gtgggcttgg tgaccgtatc
    19741 gcttcgaagg tcaatcatcg attccatgct ccaggaccga gccggcgcga ggcttcgcgg
    19801 tccgggctcg gggttctggg ttcagtccac gcggctgccc cgtctgtttc cggttcactc
    19861 tgcttgcctc agccttgctg cctggtcacc atcctgtgtc cgtcattccc cccgaacgcc
    19921 tcaggcgggc cgccttttgc gggccgcaac ccaagcgcac cgcgatccag aaacatgcgc
    19981 gcaagccgca ccgatatccg gccgtaaggt tctaatcacg ctccttgtct gccttcacgg
    20041 cacatgcggg aacttgccgt cgatctctgc tcccgccacc tcgacgagga aatctgcgat
    20101 ccggccacag accagatcca ggtaccgacg gcccttctcg gccgaggcct tcgacgggtc
    20161 gccggaggca caatggttgt tgattttcgc aaagtcacgg ctggtcagca cccaaccttg
    20221 ccgcatggcc tcgaagcgaa acggctgaac gccgccgtcg cctgccacgc ccctttcaat
    20281 gagctctgga tacagggcca ttgccaccga cgtctccatc tcgccggcgt gatcgtcgcg
    20341 agcctcaaag atctccgcgt acttgtccat gccgaccctc caccagttgc acaggaaaac
    20401 gtgggtgtcg atctcggtct gcaactggcg gatcaaaggc atgaactcgt tgccgccgtg
    20461 gccgttaagg atcagcacct tgtggatgcc gtgcttgcgc agcgagacga tgatgtcccg
    20521 gaccagcgca tcgagcgttg cctgcgaaac gtggatggtc agcggatagg ccagcaagtt
    20581 gcagtccacg ccgtacggaa tggtcggcaa gcaaatcacc gactgacaac gatcccacgc
    20641 catcttactg gcagtccggg ccacgtgcgt ggtgtgacgg tagtcctgcc cttcgggcag
    20701 atgtcgattg tgggcctcga tagccccgat cggcagaacc gcgacctcat agcagcgttt
    20761 cgagacccgg tgcaggttgg ttgcggtcaa gtcccattcg tcaatcatgg atcgatcctc
    20821 ggtgatcagc gagacgtcct gactcacgtc gcttcccccg tccttgtgcg gagcaattgt
    20881 agacgttaca catgtggacc gaaagggact cgctcaagcg ggcaacccgc ggcttgtatg
    20941 gccagggcaa ccgttccgct gacgacctcg caaccaagga agatccgaat gccccgtttg
    21001 acgtcagcca tcgttgccat cgttgtcctc acaacccccg gccctgcggc tctcggagcc
    21061 cctcccccgc tggcccccgt cgttgagtat ccgatccatc cggacggtgg catcgtctat
    21121 gaccctctga aacgcgaagc cgatcgcacc ggccaactgg aacgataccg gagcaccccc
    21181 agtaacagcc aggagatccg gctggccaac tgccgcctgc gagtcgaggt gccccgcaag
    21241 gccgccgcct acgaagtcat cccgatcccc tacgaactgt catgggaagg cggcgcgccc
    21301 gcgttcccgc tggcggtcga ggtgacggct ttcgaggacg agtcccgtcg aaaagggcgc
    21361 gattgccatg atctcaacct gcccggcaga attgatctcg atgtcgagta cctgggctcg
    21421 attaccgcac atcttcgacc aaacggccga cacaacctga aggccgacat gtccgaccag
    21481 cccgaaacgt accccccttt caaccggaga ccgttcgtgc gctcgggcgt gctggaggcc
    21541 ggcgacctgg tgtggttcaa gctgcgatac accaacaccg gcaacaccat cctcgacccg
    21601 gagggcctcg gcgggtgctt gttctacccc caactgctcc agaaacagga ggacggacag
    21661 tacactgccg tgggacaggc gtacaacctc tattatcgcg atctggagta tctctatccg
    21721 ggcgaatccc atgaaatctg gctgcatctg accagcaaca tgcccggtta catggagggg
    21781 gcaggcaaga gcggcacccc gcagggcttc ggcatctcgc ccggcgagta catgctgcag
    21841 cttcggctgt ttgctcgctg ttacaaaaca tcggatgact tcctcaacat ctgggaaggc
    21901 ccggagatgt acgcctggga gatgcccttc tcggtcgagg cccaagcccg cgaggcccct
    21961 gtcctgcccg gcacgaagac actggttgac ggcggcgatc ccgacaaaat cacgcggttc
    22021 atccacacct tcgaggagtt tatgagttcg ttcgactgtc acctgtcggc accggcggac
    22081 ggaacgagcc gcatcggcgg cacgcttcat ctgcaggtgg ccccatggac gagccaggtg
    22141 gtggccaagc tcatcgccac cgggccggtg gccatcgcga cggccggtat ccccatcgag
    22201 attgacgcga cgtcactcaa ggtgcgattc aacccgaacc cgggaacttg catggttcgc
    22261 gacgggcgcc gcatcccgat catcacctcg cagaccatgg ccgacatgcg aaccaacgtt
    22321 caaatcggcc cgttccccga aaaacacatc gtcgatcgcc tccgcgagat gatggactgc
    22381 gggatcaacg tcatcgcgcc caccaatatg ccctggctct acgatgacat gcgcaatccg
    22441 aaatcgaact accagggcga cgccttcaag tacgtgctcg atgtcgcccg ccgcgaggga
    22501 atgacggtcg aaggctgggg tacgtacccc ttcgaccggg caaatgttcg agacatcgcc
    22561 gcgtggatca ccggcgaatc gatcccgatc gagcgctaca cgcttggcaa atcggccatc
    22621 agtctcaccg aaccgatgct gcctttcgcc aactccgtgg cctggctcta ccagttccat
    22681 cgctggggcg atctgtatct gcaggttgaa cgcggcgacg tgcccatcag cgtcgaggac
    22741 acccgcggct ggatgcggca ggacgtgaac gtgcgccatc cgatgggcga ccagactgtc
    22801 aaggcgttcc gcaagtgggt ccgcaacaaa taccgcacga tcgaggcggc gaacaaggcc
    22861 tggggcagtt cgttcggctc attcgatgaa atcgaccccg aaaaagaccg cgcacccaac
    22921 cggttcgggc acaggtggga atacacggac ccgaagcatg ccttccgcga ctggaaccag
    22981 gccgtctgcg atctggatga gtttcgcacc gagctccgcg tcagcaacta ccgtgacacg
    23041 ctggccctgg ttaggaggga gatcccgcag gcggtcatct gcctgcgaac cgaaggcggc
    23101 aacgtattgg tcgacggcat cgatcccacc gaccccaacc cacacatgcg gcacgtctac
    23161 tacagccagc ggcgctgcgc agccatcgcc ggtatgctgc agaagtccgg cctgatccgc
    23221 taccactcgg attacacgac cgtgccgtat accccgagcg aattgcgccg gctcgtgcgg
    23281 atggccgtcg agcagggcat catcccatcg tacttccctc agttcgacaa catgcgcgac
    23341 atcgccatta acgagcgata cggaaccgag taccagatcc actacaatct gcccgaaccg
    23401 cgaaagggct acatgatgca ctgcctgacg gcactgtacc cctggttcac cgccgttcat
    23461 gagtccggcg gcattccggg cattctctgg gaggattacc agtgcgacgg ctttgccacc
    23521 gagacgcaga agcgggaaat gcggctgttc aaggagaagc tgatcgaggc catgtcgacg
    23581 cccgaggggc agaaggcgct ggccgaaaac gttagaatgc cttcgcaaga atggcgcaag
    23641 ggcgcgcggg caatgtggag ctacaagctg ccgaagtagg gcacggtcgg agacctgccg
    23701 ctcctcgctc accaatacgg caggtcgcgt ccgctgcggc gacctcgacg ccgccttacc
    23761 acccggcccc gtcgcttttc ggacaggttc ttgagtctgc ggcttgcgat gccgcagatt
    23821 gcggcaccag ggcaaaccat ctacgccgca ggtagaacgc aacgttgacc aggctgatca
    23881 gcacgggcac ctcgaccagc gggccgatca cggccgcgaa ggccgcgccg tgattgatgc
    23941 cgaacacgcc gaccgccaca gcgatggcca gctcgaagtt gttgctggcg gcggtaaaag
    24001 acagcgttgc ggatttcgcg tagccggccc cagccttctt gcccatccag aaactgacta
    24061 ggaacatcac cacgaagtag atcaggagcg gcacggcgat caataggaca tcaccgggct
    24121 tggtgataat cttctgaccc tgcatcgaaa acatcaccac gatggtgaat agcagggcaa
    24181 tcagcgtaat cggcgaaatc ttcggaatga acctgccgtg ataccactcc ttgcccttgg
    24241 ttttgacgag cacaagacga gtcagcatgc cggccaggaa tggaactccc agatagatga
    24301 atacactctc ggcgatctgg ccgatagtga tcccggatgt gtccgctcct tgcagtccca
    24361 gcaacggcgg caggacagtg atgaacagcc acgcgtatac gctgtagaac aggacctgaa
    24421 agatcgagtt gaaggccacg aggccggcag cgtactccgt atctccctta gccagatcat
    24481 tccagacaat gaccattgcg atgcagcgcg ccagaccgat cataatcaga ccgatcatga
    24541 attccggttg cccgcgaagg aacacaatcg cgagcaggaa catcaggatc ggtccgatga
    24601 cccagttctg cagcagcgac agacccagga tcttgatgtt acggaaaacg tcgcccaatt
    24661 cctcgtactt caccttggcc agcggcgggt acatcatcag aattaggcca atggcaatcg
    24721 ggatgctggt ctgcgtgccc tcgggccgaa gcgaactgat ccactcgccg acgccgggca
    24781 agaaataacc caggagtact ccaacgccca tggccaagaa gatccacagc gtcaggtaac
    24841 gatccaggaa ggacagtttc ttggtcagtc cacttgacat cgctcttgct cctgttcgct
    24901 attcaaatcc tgcatttgca gccgcttgcc tcgatcaatt accaggcttg acagcataga
    24961 ccttcacgct cgcggcataa tcgttgatgt tgtagcggct cagcaactgg gccattccct
    25021 tgtgaagatc cccagagccg cagtcagcgg gcttcgggca gcaacaaccc gatcgggcgg
    25081 gctggttggc gacggggagg ctcggtgtcg gcgagcagca tcccgatgaa ttctcgacct
    25141 tcgcgtatgc gttcagatcc gcaccgctgt cgatcacctg gacatgggcg aaacccgact
    25201 cgcgcaaacc ggcctcgtaa tccgcgatga ggatcgcccc ggcgatgcac cccacgtacg
    25261 ccatcacatc ctcggcgacc gccttgggca attgcttctt gagcgcaata tcgctgaccg
    25321 ccagccgccc gccgggcttg agcacccggg aaacttcgcg aaacaccgcc cgcttgtcag
    25381 gggccagatt gataacacaa ttgctgatca cgcaatcaac cgaagcgtcc ggcaagggca
    25441 gccggtcaat ggtcgccaga tgaaactcga cattcgcggg catcttgccg cctgccgatc
    25501 gagccgcgtt cttgcgggcc agctcaatca tctccggtgt catgtcgatg ccgatgactt
    25561 tgcctgtcgg cccgaccttg ggggccgcca gcagcacgtc caacccgccg ccgcagccca
    25621 ggtcgaccac ggtttcgccg ggcttgaggc tggcaaatgc cgtcgggttg ccgcacgaca
    25681 agcccatgtt cgcctcggcc gggatggccg ctaggtcatc tgcactgtaa ccaaaagcct
    25741 cggccacctg ccgcacccca tcctgccggc tcgacaggcc gctgcccgcg accgccgcat
    25801 acttcgactg cacggttctg ataatcttgt ccgacacgtt tatcctccct gaatcgccgc
    25861 cttctcctgc tcgcgcagat attccccgac ccgtgcccgg atctggtcgc gcacccgtcg
    25921 ccagacaacc agtttcgcct catcatcgcc tggcaaatgg ggaggatcct cgaacggcca
    25981 atgcactcgg catttggcac ccgggaacga cggacatgcg gcagccgcgt tgtcacacac
    26041 ggtcacgacc aggtcaaacg gctggccgtg gaactcggtc acgttcttgc tgcgctgata
    26101 atcatctagc aagcctccga gaggtatgct gttggttcgt gttcagg
//
LOCUS       DAOTPW010000050        25933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1968_length_25933_cov_5.384999, whole genome shotgun sequence.
ACCESSION   DAOTPW010000050 DAOTPW010000000
VERSION     DAOTPW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 25933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25933
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1968_length_25933_cov_5.384999"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(385..855)
                     /gene="bfr"
                     /locus_tag="P5316_06490"
     CDS             complement(385..855)
                     /gene="bfr"
                     /locus_tag="P5316_06490"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRR84643.1"
                     /translation="MKGNDKLIAVLNDLLADELTAISQYMVHSEMCDGWGYEALHKSI
                     EARAIVEMKHAEKLIGRILFLEGTPQVDKLKKMQIGSDVPKQFANDLEAEIGAWKAYN
                     DAIKLANDVGDAPTKHLLEEIVRDEDAHIDDLEEKQDQIKQMGLQLFLANQVEA"
     gene            1332..1423
                     /locus_tag="P5316_06495"
     tRNA            1332..1423
                     /locus_tag="P5316_06495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1366..1368,aa:Ser,seq:gct)
     gene            1707..2066
                     /locus_tag="P5316_06500"
     CDS             1707..2066
                     /locus_tag="P5316_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84644.1"
                     /translation="MHLEIFVDRRRKHPYVYGLFPETFRENGKMCHRTRGRVTGLSLE
                     QLEAMRDFVRQGCPKSEEPRSPSRPPPTTKKSRSAISSPLRTRVPNPVPTPVKSKTEL
                     RKMATKDSHQSIGEIAP"
     gene            2268..2537
                     /gene="rpsO"
                     /locus_tag="P5316_06505"
     CDS             2268..2537
                     /gene="rpsO"
                     /locus_tag="P5316_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRR84645.1"
                     /translation="MAITAERKRQVVTEYRQHERDSGSPEVQVALLTERISELTEHLK
                     THKNDHSSRRGLLKLVSRRSGLLKYLTNRDRERYQKIINRLGLRK"
     gene            2734..4842
                     /gene="pnp"
                     /locus_tag="P5316_06510"
     CDS             2734..4842
                     /gene="pnp"
                     /locus_tag="P5316_06510"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRR84646.1"
                     /translation="MAIVKVECEVGGRKLSFETGKLAKQADGAVVASYGECVVLGTSV
                     VGEPRDEGDFFPLTVDYREKAYAAGKFPGGFFKREGRPSNKEILTMRMIDRPIRPLFP
                     EQFVNEVQIQTLVLAADPTLDPDITAINAASAAMAIGSTPFEGPVGAVRVGYIDGQIV
                     INPTYDQVEASELDMVLAGHKDGINMIEVGAREVSEQIVLEAIAQGFEAIQTICKTIA
                     ELRQAAGKPCSWQPPEPNHELSRLVREKAYDQMWQRRQIPGKLERQNAIKELYDQILN
                     ELSPEDVDTPPYVRSEVMHCLQKIEEEVVCKQILETNRRSDGRSPTDIRPVSGEVGFL
                     PRTHGSALFTRGETQAIVVTTLGTARDEQIIDGLVEEYSKKFMLHYNFPPFCVGETRR
                     IMGPGRREIGHGALAERSLEAVLPAVDTFPYTIRLSSEIMESNGSSSMASVCGGTLAL
                     MDAGVPISQPVAGISIGSVHDDSRRVLLTDILGEEDHFGDMDFKVAGTGRGITGIQLD
                     LKERFISQDTIAQVLERARDARKFILREMLKIIDRPRPKISDYAPRILTITINPEKIG
                     AVIGPGGKGIRSIEAETGATIDIEDDGKVTISCLNMAGAIQARDMIEQISEGVKVGKI
                     YTGRVTGIKDFGAFIEIAPGQDGLCHISELSDGYVKSVTDVCKVGDVLKVKVILIDDQ
                     GRVKLSRKAVLREETAEAKK"
     gene            5233..5448
                     /locus_tag="P5316_06515"
     CDS             5233..5448
                     /locus_tag="P5316_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRR84647.1"
                     /translation="MATGTVKWFDCKKGFGFIRQPEEDKDIFVHYTSIEGEGFRSLKD
                     GEAVEFELVESEKGLQAHHVRRAKLTV"
     gene            5646..7094
                     /locus_tag="P5316_06520"
     CDS             5646..7094
                     /locus_tag="P5316_06520"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR84648.1"
                     /translation="MIQATARAPYNTNFDRPVVDGHRKKGRATVAETAFILIVEKDAL
                     RAEAMVKDLSEQGHVCLVVPSGSEALDSIRVRQPDVIVADESLFDKGNDNLLRQARRL
                     APQAEIILVTDGARRPPDDSTTEDAVRVYRRIPRQMNADDARATIFAAADQATRNRHR
                     RALQEQVERRFEFEGIVTGNPQMERIINMIRRVADSKLTVLILGPSGSGKELAAQAIH
                     RHSPRRNKPYRAINCAGLNENLLESELFGHVKGAFTGAISDRKGLFEVADGGTLFLDE
                     VGDMPLPMQAKLLRVLENGEILPVGSTEVRKVDVRVVAATRRDLRAMIDSGAFRDDLF
                     YRLHQATIRLPALRERRDDIPLLIDHFLKEACRLHNKKVDSISPEAVRRLTSYSWPGN
                     IRELRSVIDVMVVMADRPQLEIEDLPENIRGSTDIVLAGTAGTAGLTMEQMERIHIAN
                     TLKLTGGNREKTAKILGIGARTLYRKLREYGL"
     gene            7216..8526
                     /locus_tag="P5316_06525"
     CDS             7216..8526
                     /locus_tag="P5316_06525"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR84649.1"
                     /translation="MLFLPQHEHRSRPAAVLIVAILHAGGCGRELMPTPNICADPRRN
                     LFQDVPPQHRSNRVPVLYATDRVPFQHKKRGLQYDVNRSQSLGFGTCMVEIGKNLSWE
                     ELVVQSRMRRRTRPLPLSVVGIDEKGRYPDAVNFRVVDGKPVVDEAAREKEKQANEAL
                     QALLSEHLAVVPIKEAYVYIHGVGNTFDYAAGTIAELWHFMGRQGVPVLYSWPAGYEE
                     GIIQSYTHDRESGEFTVFHLKQFLRTLASCPDLQKMHLIAHSRGTDVIATALRELHIE
                     CTAAGKDTRSQLKLGTLVLAAADMDIEVTTQRISAEGLPLVPECSTVYTSEKDLAMKL
                     ADFLFGSLRRLGQLRGNDLTPEQQRMLAAITHLQFIDAKVSSGFIGHSYFYENPAVSS
                     DLILLLRDRRKPGAENGRPLKKRDDGFWEIRDGYPVFNDESATK"
     gene            complement(8626..9519)
                     /locus_tag="P5316_06530"
     CDS             complement(8626..9519)
                     /locus_tag="P5316_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRR84650.1"
                     /translation="MAEDRLPVLLDTDIGSDIDDAVALTYLLSQPRCELVGITTVTGE
                     PAVRAQLADAICQAFGRKDVPIHSGAGKPLLVPQKQPDVPQRTVLSRYPHRETFAANT
                     AVDFLRQAIRSRPGDITLLTIGPLTNVGLLFALDPEIPALLKQHVMMAGVYADRPPGC
                     GLTEWNVTCDPHASAIVFGARPPVVRCVGLEVTTRCEMPAEEFRRRFSERPRRIVGEM
                     AEEWFKHRPVVTFHDPLATADIFQPGILSYRAGLVQVELESDKLRGLTHFDPDAPERP
                     HQVAVGVEPDAFFEHYFEIVR"
     gene            complement(9529..12168)
                     /locus_tag="P5316_06535"
     CDS             complement(9529..12168)
                     /locus_tag="P5316_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRR84651.1"
                     /translation="MKLGHLLMWAMVLLAWAVAPSGLMASEKAPSVAVELLSEQTAWV
                     PGETVNVALRFKVEDGWHIYWRNAGEGGLEPSFKWEVPGGWRVGMLAYPFPKRYVDAG
                     GEHTFILEGEPTILAPIEVPREAVAGSQVTVGVDVTWMACRKACFLGNKKLAITLPVA
                     GGEVKAEPANEDTFQLARSYLPQPAEKAKYLKRLEAVADTHKAKPGTKFEVAVVFDVA
                     EGHHINSHRPLGEFLMPTDVFHEKTEGLYIGRARFPAGKVESSGLPGDQLSVYRGRVF
                     VLLPVEAEPGLTGSEVRIRGVATYQACSDQTKVCFPPMAVEWELVLPVAKAGETPQPA
                     HGELFAAARRGMPPQELKTPGRDSGRSEKIGPGKDSGPSELDSGPSELDSGPSELASG
                     PSESDRRADEGVGPTSGLGPTTSAPSLPLLTSDSWLGRIQASLAGLGFVGYLIMSLVG
                     GLILNLMPCVLPVISIKVLSFVQQARESRMRVLTLGLAFGAGIELSFIVLGLLIVGLG
                     RTWGGFFQYPQVIIGLASVVTAFSLSLFGVFALFPPRIVGDLAGQVEGEGHLSAFGMG
                     LLATLLGTACTAPFLAGAVAIAAQQPAVVGMSIFVTAGFGMSLPYVILAAKPMWVRFI
                     PKAGPWMKTFEHLMGFCLLATVVWLLNPLAAQLGGYGLLLAVLFLLLVSLAAWLYGKV
                     EYGAAPRRKITYYGLAAAVVVGGWWLCFHRWSSIPDLIERQRALVLGAWQPIPAGSRP
                     TDEFAGLVWKGDKIPWIPYTRERARRAVDTGYTVFIDYTAEWCVNCKANEKAVINTQA
                     VHEAMKRLGVLPFKADYTLPSREIAEDLKRYGSGGVPLYLIMPAGKPDHVIRLDEVLT
                     ASSLIGGLERAGPSKPSLQAGGS"
     gene            complement(12655..13221)
                     /locus_tag="P5316_06540"
     CDS             complement(12655..13221)
                     /locus_tag="P5316_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84652.1"
                     /translation="MKRMTVVVAVLMMAGIGYGATFSVTSGGSPVKIVAPGEEFMVQI
                     NLDPQGMELVSWGVVIEAPDGYKIPGAPGVAAYAPGVAQGWSATDGNTAWQGSNWPRS
                     FPAGPIFNVFGDVFGITAAGPVFEMKVRAPMDPAALMGEITVTGAYVGDTDFVEHSDV
                     NVVGLRVVPEPVSALLLLAGVPMLRRRR"
     gene            complement(13385..14992)
                     /locus_tag="P5316_06545"
     CDS             complement(13385..14992)
                     /locus_tag="P5316_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HRR84653.1"
                     /translation="MRVTAIGAIVLMLGGIATAQTQWSEIDYTIEIGGTNYADDVHAG
                     DYKAFVPGSTTPAPQTGIVTWDVVAAAAGLDQTEQYMIGGIANMVFDIQLFKQDGENW
                     VPAAPKKFFSTMNDGSELPPPLTGQNPFERAAFCLGWNVDPEYDGYFTVEYGPPNGPG
                     RLYDVPADGGAFMDRVQFPSTKYHGGGRMKAPAYFPDCNGNLINDDTEENGTTDADSN
                     GILDICENLGVELSGAETLDPSKLSGMGIGYSAFSISTANNSAGVGLAKQSPENPGPM
                     VVWNPDGYVGLGALPVAEGQIDMSNLENGTYKLVLTVPANSNNVIPADYMGTAGGFAE
                     AVTEAIAPAEVVFEWQGYVPVCQEVSPIAWNSLRTHGAAGQQAIVLNPADGTATVEPR
                     NNGIQKISIQFSGDLTKANYAAGGVSITGGLVVTAESLATTAVANDTLILTVTGNVDK
                     TCYTINVAGATQCTVGDTTCSVLALAGDANQDKRVSATDVALVKSRVGQSVAANVRCD
                     LNCDGTISATDVALAKSKVGLPTYTCP"
     gene            complement(15709..16965)
                     /locus_tag="P5316_06550"
     CDS             complement(15709..16965)
                     /locus_tag="P5316_06550"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRR84654.1"
                     /translation="MNKPGARISKRSSRLRRPADRSAVGLVLGLMAGTSGDGIDLACV
                     RIEGVGARMRPRLLWHRHTGFPPSLRRRLLAVMAPGETTTQEIAHLHADLGEAFGKAA
                     AWAIGLARGSERPSLIGLSGQTVCHLPGPTGRTVTLQLGEPARVAARTGLPVVAEFRQ
                     SDVAAGGQGAPLVPWTDWALFRHHRISRAIQNIGGIANVTWLPAGGDASRVIAFDTGP
                     GNMVIDAVVRCITGGRETMDRGGRRAAKGRVLGDVLDQWLRHPYFEIKPPKTTGREDF
                     GKPFMTREIKHLAAAGSRPDDWVATATALTARSIAQAYRRFLPDVATLSARRRKIRGG
                     SALPAMEVIVCGGGARNPTLMLMLQAELRGATVRRIEEFGISAQAKEAVSFAMLAKAY
                     IDGVPGNLPQVTGARRPALLGRLVWP"
     gene            17306..18847
                     /locus_tag="P5316_06555"
     CDS             17306..18847
                     /locus_tag="P5316_06555"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84655.1"
                     /translation="MAGAESNAVRPPMAVFRTVLTIFFLLPATFVRADSRWKLIYYRQ
                     YGNQLNANNRTIENHCFNDNGTKAAGIGVTNLSNSGWAIFGTTDFTGWNRLNPLDPNN
                     CAYDIRIYDPSTPTDQSPNFNWQNTQPPWPDYYSYITWWMKVSDDTRVRAFPQKPVYH
                     YDLVDGLNQAGGFTGGCIPTGWVEAPGANSDYRELPTGGWATYQAQTFVVPAGINRIV
                     AASAFLTRGSSPPGPKFTYRATIREGSPTGTQIGPATVSREVFSGEFKEVAVCWPLNG
                     VPVTEGQTYALHLEATDGQGFNVYRTVNNNYPNGSLWHGDTHVAEHDMVAVVVGVGYD
                     ASPPHISRNPALLTKSVVRKNNLPNDTFTIANGGGGTLNYTINDNASWLSVDPAAGSS
                     TGETDTISILYGTAGLAIGSYSGTITINATGADNTPQTVVVNLSVTAPPFAPCDFDTD
                     HDVDQEDFGVFQSCYTGPGVNQTDPACSGALLDLDTDVDYEDFARFLACMGGPDIPVD
                     TTCAD"
     gene            18929..20752
                     /locus_tag="P5316_06560"
     CDS             18929..20752
                     /locus_tag="P5316_06560"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84656.1"
                     /translation="MNTCQPPRWLNAGLMRLVCVGLLMPATVAVADGTVSGQCRFEQI
                     KGRSIWYTNLHEWNAFLIPSWSMFADPLSYRVDHGNYTFTNPAGDYTLYVNQPEFFGR
                     AKVVRDVQIINGQTVTVHPSLNLDYSCYYKNEWPSGWGTPWYQTFIATGTSVNKIVYS
                     CASGDTGTINVSLLKDTGGNVTTWTQVGVTKSSGSSIGESWVGYLSGQLPTVPGERYA
                     IRLAGEASTPFSPYWRTDNGDGYAGGQAFDPNGNPLNKDLCILVFSDNDGTVIPYMKT
                     TGGMGSTAWWEWQWGQTFKATGEALAGVDLWISHGGDLQWNMRTEVKVYSNPPTGNPP
                     TPSGLVGLVKRGRGAWQAGGAAMLGIGYAPGEVPLVAGNTYYIEITPYGGDAIGYEAY
                     RFDQPADAYPYGHAYSNRNPQPDIDLSMTIMEYAVSGPPPQIERNPASFTRSVERGKN
                     LPDATFTVRNAGGGVMPYTISDDAAWLSVAPNSGTSSGEVDPIGILFNTSTLAVGSYT
                     GTITISAPNAANPTETVIVYLTVTPPLFAPCDFNTDHDVDQTDFGVFQACYSGAGVTQ
                     TDVACTGADLDGDGDVDMDDFTRFVGCLSGPGVQADTLCAG"
     gene            21666..21992
                     /locus_tag="P5316_06565"
     CDS             21666..21992
                     /locus_tag="P5316_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84657.1"
                     /translation="MKHFAITLVAVCALVLAAFAARPLLAGPPVPAAGMSCGDNCGTD
                     RYETKCGDNCKCGDNCQCGNDCKCGDECKCGDDCKCGDNCKCKCKDKSEDKSKGKCKD
                     KCRDKK"
     gene            22451..23314
                     /gene="ispH"
                     /locus_tag="P5316_06570"
     CDS             22451..23314
                     /gene="ispH"
                     /locus_tag="P5316_06570"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRR84658.1"
                     /translation="MEVRLARKHGFCFGVEQAIELAEQTLRDNPPGRVAALGPVIHNP
                     QVVRRLEQEGLNQSAHLDNLEAGTTVLIRSHGVGPEIYEMARQRGINLVDATCRLVKR
                     AQTVVKQLHEEGYQVVMIGDANHPEVKGVIGYAPNVIVVEQEEDLANKIPPRARLGIV
                     AQTTHAPEHVARMIGAIAALPFQELRVVNTLCLEVVRRQEAAVELAREVDVMFVLGGL
                     TSANTCELARLCQGEGVRTYHLETWADFRPEMVAGCKVAGITAGASTPDDTITEFAEN
                     LRRYNPIKQPD"
     gene            complement(23477..24223)
                     /locus_tag="P5316_06575"
     CDS             complement(23477..24223)
                     /locus_tag="P5316_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84659.1"
                     /translation="MVHRLAAALLCAGTVCVLAAGESNIDPNHRFAWAENVGWLNWHG
                     ANSNVRVHPSFLSGFVWAENVGWINLGNGRPANGTAYANTNGEDCGVNIDALTGDLSG
                     LGWGENIGWVNFDTWAALGTYGRQARFEYAPAGNYWAGRFRGYAWGENVGWINLDDSV
                     AYVSVVSPDCRDPFADADGDGDVDQADFAIMQICVSGDLTPALAGCVCFDRPEWGFPL
                     GDNDVDANDLAAFEACATGPGLVVPSGCDG"
     gene            complement(24277..24747)
                     /locus_tag="P5316_06580"
     CDS             complement(24277..24747)
                     /locus_tag="P5316_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84660.1"
                     /translation="MSSRRIRGLIVTGMVVCLCLVLWVQAGDLNPPVGPVSPTMKTLE
                     QLSAEHAQLASAIAGIPGGGGGGVKQVVRGVIELASGAVEGSANFTPEIMPSKSMVVL
                     SDAVVVNMSDARNTLAGRTAACLMDLTASSLTIRVDEVPLTGIRRVSYQIIEYN"
     gene            complement(25279..>25933)
                     /locus_tag="P5316_06585"
     rRNA            complement(25279..>25933)
                     /locus_tag="P5316_06585"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         5502 a         8368 c         7282 g         4781 t
ORIGIN      
        1 aagccggaca ccgaccgccg accacccccc gcgccaaacg aacccaaccg agatcgtaag
       61 tctttatcaa ttaaacactt aaggaaaccc gtggccggtt ccccgtcatc cgttccaccc
      121 cgaacatctc accccagccc tccaacccgc cttccgggag gtcgccagcc tcgctcggtt
      181 gacctgcaga gcccgccatc cccccaccca gccaccttac ctttgcgcca actgggccct
      241 ggtcgatcgg ccatctcaga actcggatat ttcgccggaa cagtaccagt aggagaccga
      301 ggaatagatc ccccggcccg tcaagaaaag catcagggca cgccaatccg cgatcgcggg
      361 ccatcctcga tggttgtcgc gcctttacgc ctcaacctgg ttggccagga acaactgcag
      421 gcccatctgc ttgatctgat cctgcttctc ctcaagatcg tcgatgtggg catcctcgtc
      481 ccggacgatc tcttcgagca agtgcttggt cggagcatcc ccgacgtcgt tggccagctt
      541 gatggcgtcg ttataggctt tccaggcccc gatttcggcc tccaggtcgt ttgcgaattg
      601 cttgggaaca tccgagccga tctgcatctt cttgagcttg tcgacctgcg gcgtaccctc
      661 caggaagagg atgcgaccaa tcagcttctc agcgtgcttc atctcgacga tagctcgggc
      721 ctcaatgctc ttatgcaggg cctcataacc ccagccatcg cacatctccg aatgaaccat
      781 gtattggctg atcgccgtca gttcgtcggc cagcaggtca ttcaacaccg cgatcagttt
      841 atcgttgccc ttcatcgcat acctccatgc ccaagcgggc tcttaagttc ttccggaaat
      901 ccgaggccac aatcaggtgg cactatacga aggacggccg gaggcgtcaa accgccggaa
      961 aaatcacgct ggcgggcagc aaagcgctag gccgacgtga ggccaaaagc cctttccgag
     1021 accccgaaag ccgaccggcc ggaagctgat cccgccacag gtttcggggt aggccgtaag
     1081 ttcttgacca tacgcgaggt aaacgataga ttaggggtgg tcgctgtcgt cgagcatgca
     1141 ggtctggcac catggcaaga taccgtcccg cgtcgacatg gcgatgcatc catgaaccga
     1201 gacggtggct gtgccgacgg gcctgtgggt tgccactgtg ggggcgaaca caataggagc
     1261 ctatccaaga accctgaaag ccgagcggcc gcaagccggt cccccacagg ttctgagata
     1321 ggttctgagt tggagaggtg gctgagtggc tgaaggcacc ggtttgctaa accggcatac
     1381 ggccgttaag gctgtatcgc gggttcgaat cccgccctct ccgttccttc gtcactgtcg
     1441 cctgtcaagc gcacgcggca acttaccccc ccatgaaaca aggcaagtat ctgtaactgc
     1501 ggtggttggc tgccgttctg cagtccaaat gtagccatgg gggcggttca cccctcccac
     1561 aaaacgctat aaatgattgt cgctaaagta gttgtctgct gttctgcatt tcaaatgtag
     1621 ccatgtacat ttttgctctt attcttgata tcggctgtac ttaatgatat attttcggtt
     1681 atttgggcca tgtacggagg cagctcatgc acctcgagat ctttgtggat cggcggcgta
     1741 aacatcccta cgtgtacggt ctgtttccgg agacctttcg cgagaacggc aaaatgtgcc
     1801 accgcacgcg agggcgagtg acgggcctgt cgctggagca gttggaggcc atgcgggact
     1861 ttgtccgcca aggttgtccc aagagcgaag aaccgaggtc tccatcgaga ccacccccga
     1921 cgaccaagaa aagcagatcc gcgatctcct cgcctctgag aacgcgagtc cccaaccctg
     1981 tgcccacacc cgtgaaatcc aaaaccgaac tcaggaaaat ggccaccaaa gactcgcatc
     2041 aatcaatagg ggagatcgcg ccttgactgc ggtactcttt cagattgagg ttctgaaaaa
     2101 accgcccagc ctgtcaccct tgcaaagctg ccccgaccgg catatagtgg tgcatcggaa
     2161 acagaagaaa gggtaaggga attgttaggt ttggcggccg gccaaggccg tcaaatggct
     2221 ggaacagttg gttcctggtt ttggcattcg gtctgtggta ggcggcgatg gcgataacgg
     2281 ctgagcgcaa aaggcaagtg gtaactgagt atcggcagca cgagagggac tccgggtcac
     2341 ccgaggtcca agtggcgctg cttaccgaac gaatctcgga gctgaccgag catctgaaaa
     2401 cacacaagaa cgaccactcc tctcgacgcg gcctgctcaa gctcgtgagc cggcgctcgg
     2461 gtctgctcaa gtacctgacc aatcgagatc gcgaacgtta ccagaagatc atcaaccgct
     2521 tgggcctgcg caagtagtat cccgcgactc ccagcccgaa tcgggttccc cccggtcagc
     2581 ccataacaac cctccccatc ttccggcagc ggcagtacag cgtcaggtgg accgccagtc
     2641 cccctttgga gggtccaacc ctcagtgtga ccggcttccg gatagcgcgt atcggtgtca
     2701 gttcaagaaa ggcaaagagt aggtgctggg ataatggcga ttgtgaaggt ggaatgcgaa
     2761 gttggcggaa gaaagctgtc ttttgagaca ggtaagctgg ccaagcaggc ggacggagcg
     2821 gttgtcgctt cctacggcga atgtgtcgtg ctgggcacct cagtggtggg cgaacctcgc
     2881 gacgagggcg acttctttcc tctcacggtg gattaccgcg agaaggccta tgctgccggg
     2941 aagttccctg gcggcttctt caaacgagag ggtcgtccga gcaacaagga gattctcacc
     3001 atgcggatga tcgaccggcc gatccgtccg ctcttccccg agcaattcgt caacgaagtg
     3061 cagattcaga cgctggtgtt ggccgccgac ccaacactcg acccggacat cacggcgatc
     3121 aacgccgctt cggccgccat ggccatcggt tcaacccctt tcgaggggcc ggtcggggcg
     3181 gttcgggtag gctacattga cggccagatc gtcatcaacc cgacttatga ccaagtcgag
     3241 gcctccgaac tcgacatggt tctcgccggc cacaaggacg gcatcaatat gatcgaggtc
     3301 ggtgccaggg aagtcagcga acaaatcgtc ctggaggcca ttgcccaggg attcgaggcc
     3361 atccagacga tctgcaaaac catcgccgag ctccgacaag ctgcaggaaa gccttgcagt
     3421 tggcaacctc ctgaaccgaa ccacgaactg tcccgcctcg tgcgcgaaaa agcttacgac
     3481 cagatgtggc agcggcggca gattcccggc aaactcgaac gtcagaatgc catcaaagaa
     3541 ctctacgacc agatcctgaa cgagctctcg ccggaagacg tcgacacccc cccctatgtc
     3601 cgcagcgaag tcatgcactg tctccagaag attgaggaag aggttgtctg taaacagatc
     3661 ctggagacaa accgtcggtc agacgggcgt tcaccaacgg acatcagacc tgtctccggc
     3721 gaagtgggct tcctccccag aacccatggg tcagcactgt tcacccgggg tgaaacgcag
     3781 gccatcgtcg taacaacgct gggaaccgcc cgggatgagc agatcatcga cggcctcgtc
     3841 gaggaataca gcaagaagtt catgcttcat tacaacttcc cgcccttctg cgtgggcgag
     3901 accagacgga tcatgggtcc aggacgacgc gagatcggtc atggggcctt agcggagcga
     3961 tcgctggaag cggtcctgcc tgcggtggac actttcccct ataccatccg actcagcagc
     4021 gaaatcatgg aatccaacgg ttcgagttcg atggcttcgg tttgcggcgg aacgttggca
     4081 ctcatggacg cgggcgttcc catcagccag cctgtggctg ggatttcaat cgggagcgtc
     4141 cacgacgaca gccggcgggt cctactaacc gacatacttg gcgaggaaga ccacttcggc
     4201 gacatggatt tcaaggtcgc aggcaccgga cgcggcatta caggcataca gctagaccta
     4261 aaggaaaggt tcatctctca ggacaccatt gcccaggtgc tcgagcgggc ccgtgacgca
     4321 cgtaagttca ttctccgtga gatgctaaag ataatcgatc gccccagacc caagatcagt
     4381 gactatgctc ctcgtatcct gacgataacc atcaatccgg agaagatcgg cgccgtcatc
     4441 ggaccaggcg gtaagggcat ccgtagtatt gaggccgaaa ccggtgctac aatagatatc
     4501 gaggatgatg gcaaggtcac catctcctgt ttgaacatgg ccggtgccat ccaagctcga
     4561 gatatgatcg agcagatcag cgaaggcgtc aaggtcggta agatttatac cggtcgcgtg
     4621 acgggtatca aggacttcgg cgccttcatt gagatcgcgc caggccagga cggcctttgc
     4681 cacatcagcg agctgtcaga cggatacgtg aaatcggtaa cggacgtgtg caaggtcggc
     4741 gacgttctca aagtgaaagt catcctcatc gacgaccaag gacgcgtcaa actcagccgc
     4801 aaggccgtgc tccgcgagga aacggctgag gcaaagaagt gagacctggg caaacgcaac
     4861 cggctcacgg attccgaaat cggcttcgtg ccgaggggcg gccatgcaat tcgtgccagg
     4921 gcctctccgc atctgcatgc gtcttgatcg tccgcggtgt tgcgtgactc cgcaggacgt
     4981 tgcataggaa tcaggtcgga tacccttttg tggtacgtgt gtttccggac gcacgccggt
     5041 cgggtatccg tgtgcagacc gccccggata ggtcgtgtga acaggtgccc gtctcggcac
     5101 gatgttgtcg gacatgggcg ggggtcttcc ggggcagtcg gcgcaatcga attcgcgggc
     5161 cggcacgtcc aagtctgtca agatgttccg cgcggggggt gcgaaacgtc gctcttgccg
     5221 gaggtactgc acatggctac cggcacagtc aagtggttcg attgcaagaa aggattcggg
     5281 ttcatccgcc aacccgagga ggacaaggac atctttgtcc actacaccag tatcgagggc
     5341 gagggcttcc ggagcctgaa ggacggcgaa gctgtggagt ttgaactggt cgagtccgaa
     5401 aagggtctcc aagcccacca tgtccgccga gccaagctga ccgtctgagc ggccgtccgt
     5461 ttgctgccgg cgtcctcggt gatcatcttg ggcgacccca gggggaatga ccacgggggc
     5521 ccgcacgtcc cgagcatacc ttgcagccgt tacggccaag cccccttgcc tcgttgccga
     5581 ccgagaagag tcccgccggc aatcccgagc aacgagcctt tttcgcatcc aagacggcct
     5641 cccagatgat tcaagcaacc gcaagggcgc cttataatac gaatttcgac cggccggtcg
     5701 tcgacggcca tcgcaagaaa ggtcgtgcaa ccgtggctga aaccgctttc atcttgatcg
     5761 tggaaaagga cgccttgcga gccgaggcaa tggtcaagga tctgagcgag cagggccatg
     5821 tttgcctggt cgtcccgtcg ggctcagaag ccctggacag cattcgcgtc cggcaacccg
     5881 atgtcatcgt ggcagacgaa tcgctcttcg acaaaggcaa tgacaatctg ctgcgccagg
     5941 ctcgaaggct cgctccgcag gcggaaatca tcctggtcac cgacggagcc cgcagacctc
     6001 ccgacgactc gaccaccgaa gacgccgtgc gggtataccg gcgcatccca cgccagatga
     6061 acgccgacga cgcccgggca accatcttcg cggccgcgga tcaggccacg cgaaaccggc
     6121 atcgacgcgc ccttcaggaa caggtcgaac ggcggttcga gttcgagggc atcgtcaccg
     6181 gcaatccaca gatggagcgg atcatcaaca tgatccgtcg cgtcgccgac agcaagctta
     6241 ccgtgttgat cctcgggcca tccggcagcg gcaaggagtt ggccgcccaa gccattcatc
     6301 gccactcgcc tcgccgcaac aaaccctacc gggcgatcaa ttgtgccgga ctcaacgaga
     6361 acctgctgga aagcgaactc ttcgggcacg tgaagggagc atttaccgga gcaatctccg
     6421 accgcaaagg tctcttcgag gtggctgacg gcggcaccct ttttctggac gaggtcggcg
     6481 acatgcccct gcccatgcag gccaaactcc tgcgagtcct cgaaaacggc gagattctgc
     6541 ccgttggcag caccgaggtt cgcaaggtcg acgtccgcgt ggttgcagcc acccggcgcg
     6601 acctgcgggc aatgatcgac agcggcgcat tccgcgatga tctcttctac cggctgcacc
     6661 aggcgaccat tcggcttcct gccctgcgtg agcgccgcga cgacatcccg ctgttgatcg
     6721 accacttcct caaggaggcc tgccgcctgc acaacaagaa ggtcgattcg atcagcccgg
     6781 aggccgttcg gcgcctcacc agctactcct ggccgggcaa catccgcgag cttcgcagtg
     6841 tcatcgacgt catggtcgtc atggccgacc gcccccagct cgagattgag gacttgccgg
     6901 agaacatccg cgggagcacg gacatcgtcc tggccggcac cgccggaacc gccggcctca
     6961 ccatggagca gatggagcgc attcacatcg ccaacaccct caagctcacc ggcggcaacc
     7021 gcgagaaaac cgccaagatc ctgggcatcg gcgcccgcac gctctaccgc aaactccgcg
     7081 aatacggact ttgagtcaat ctctgccggc ccggccaagc ccaagagcga acggccgcgc
     7141 gtggctcgac gccgggactc ggatacgata ggcatggcac cttcaccgaa cagccctgta
     7201 ttctggagat ccggcatgct cttccttccc caacacgaac accgaagtcg cccggctgca
     7261 gtcctgatcg tggcgatcct gcatgccggt ggatgcggcc gcgagctgat gcccacgccg
     7321 aacatctgtg ctgacccccg tcgaaacctc ttccaggacg tgcccccgca gcaccggagc
     7381 aaccgcgtgc cggtactgta cgccaccgat cgcgtaccct ttcagcacaa gaaacggggc
     7441 ctgcaatacg acgtaaaccg ttcgcaatcg ctgggctttg gcacgtgcat ggtcgaaatc
     7501 ggaaagaatc tctcatggga agagctggtc gtacaaagcc gtatgcgccg gcgcacgcgg
     7561 ccgctgccgc tgtccgtggt cgggatcgac gaaaaaggca gataccccga cgcggtcaac
     7621 ttccgcgtcg tcgatggaaa gccggtggtc gacgaggccg cccgagaaaa ggaaaaacaa
     7681 gccaatgagg cgctgcaggc actcctctcc gagcacctgg cggtcgttcc cattaaggaa
     7741 gcgtacgtct atatccacgg cgtcggaaac accttcgatt acgcagcggg cacgatcgcc
     7801 gagttatggc acttcatggg tcgtcaggga gtgcccgtcc tctactcctg gcccgccggc
     7861 tacgaagagg gaatcattca gtcgtatacg cacgaccgcg aatcgggcga attcaccgtc
     7921 tttcatctca agcagttcct gagaactctg gcctcatgcc ccgatctgca gaagatgcac
     7981 ttgattgccc acagccgcgg aacggacgtc atcgcgacgg ccctgcggga gctgcacatc
     8041 gaatgcacgg cggccggcaa ggacacacga tcccaactca aactgggtac tctcgttttg
     8101 gctgctgcgg acatggacat cgaggtgaca acccagcgaa tctctgccga aggccttccg
     8161 ctggtgcctg agtgttccac cgtctatacg tcggaaaaag atctggcgat gaagctggcc
     8221 gattttctgt ttggcagcct gagacggttg ggtcaactgc ggggaaacga tctgactccc
     8281 gagcaacagc ggatgcttgc tgcgataacc cacctgcagt tcatcgatgc caaggtcagc
     8341 agcggcttca ttggacacag ctacttctat gagaacccgg cggtcagctc ggacctgatc
     8401 ctgctgctgc gagaccgccg caaacccgga gcggaaaacg gccggccatt gaaaaagcgc
     8461 gatgacggct tctgggagat ccgcgacgga tacccggtct tcaatgacga gagcgccacg
     8521 aagtgacgat ggcggcaata agccctgtcc caagcgtcga atttgaaacc gcgacgtcgc
     8581 cggataaacc tcaatgcgaa agccggagga aagtttgcat caggctcacc gcacgatctc
     8641 gaaatagtgc tcgaagaaag catccggctc cacgccgacg gccacctgat gaggtctctc
     8701 aggggcatca ggatcgaaat gcgtcaggcc cctgagcttg tccgactcca gctcaacctg
     8761 caccaaaccg gcacgatagc tcaagattcc tggctggaag atgtccgctg tggccagcgg
     8821 gtcgtgaaaa gtcaccaccg gtcggtgctt gaaccactcc tccgccatct cgccgacgat
     8881 cctccgcggc ctctcgctga atcgcctccg gaactcttct gccggcatct cgcaccgcgt
     8941 ggtcacctcc agcccgacac acctgaccac cggtggccgg gccccgaaaa caatggcaga
     9001 tgcgtgcgga tcgcaagtga cattccactc cgtcaaacca cagcccggcg gacgatccgc
     9061 atacacgccc gccatcatca catgctgctt gagcagggcc ggaatctcgg gatccagggc
     9121 aaacaacagc ccgacgttgg taagtgggcc gattgtcagc aacgtgatat cgccgggccg
     9181 ggagcggatg gcctgacgca ggaagtcgac cgccgtgttg gccgcaaatg tctcgcggtg
     9241 cggatatcga gacagcaccg tccgctgtgg aacatccggc tgtttctggg gcacaagcag
     9301 cggcttgccc gcgccgctgt ggatcggcac gtccttacgg ccgaacgcct gacagatggc
     9361 atcggcgagc tgagcacgaa ccgccggctc gccggtcacc gtggtaatgc ccaccagctc
     9421 gcaccgaggc tggctcaaca ggtacgtcaa cgccaccgcg tcgtcgatgt cactgccgat
     9481 atcggtatcc agcaggacag gtagccggtc ttcggccatg ctcgatcctc agcttccgcc
     9541 cgcctgcaag ctcggcttgc tcggccccgc cctctccaac ccgccgatca gcgatgacgc
     9601 cgtcaggact tcgtccagac gaatcacatg atccggcttg cccgcaggca tgatcaaata
     9661 aagcgggaca ccaccgcttc catacctctt caaatcctcc gcaatctcgc ggcttggcaa
     9721 cgtgtagtcg gccttgaacg gcagcacacc cagtcgtttc attgcttcgt gaaccgcctg
     9781 ggtattgatc accgctttct cgttggcctt gcagttgacg caccactcgg cggtatagtc
     9841 gatgaacacg gtgtagcccg tatcgacggc gcggcgagcc cgctcgcggg tgtagggtat
     9901 ccagggaatc ttgtccccct tccagaccag gccggcgaat tcgtctgtcg gccgcgaacc
     9961 tgccgggatc ggctgccacg cccccagcac caatgctctt tgccgctcga tcaggtccgg
    10021 aatgctcgac caccgatgaa aacagagcca ccagcccccg acgaccacag ccgcggccag
    10081 cccgtagtaa gtgatctttc gtcgcggggc ggccccatat tcaaccttgc catacagcca
    10141 cgcggccaac gacaccagca gcagaaacag aaccgccagc agcaggccat agccaccgag
    10201 ctgcgccgcc agcgggttga gcagccagac cacggtggca agcaggcaga agcccatcag
    10261 gtgctcgaat gtcttcatcc atggcccggc cttgggtatg aacctcaccc acatcggctt
    10321 ggctgccagg atgacatacg gaaggctcat gccgaaaccg gcagttacaa agatcgacat
    10381 tccgaccacc gccggctgct gggccgcaat ggccacagca ccggccagaa acggcgcagt
    10441 gcaggccgtg cccagaagcg tggcgagcag ccccatcccg aaggcactca aatggccttc
    10501 cccttcgacc tggccggcca gatccccgac gatccgcggc gggaacaagg caaacactcc
    10561 gaacaaactc aatgaaaacg ccgtcaccac cgaagccagg ccaataatca cctgaggata
    10621 ctggaaaaag ccgccccacg tgcgtcccaa cccgacaatc aacaaaccga gcacgatgaa
    10681 ggacaattcg atccccgcgc caaacgcaag accgagcgtc agcacgcgca ttcggctttc
    10741 cctggcctgc tgcacgaagg acagaacctt gattgaaatc accggcaata cgcagggcat
    10801 gagattgaga atcaggccgc caaccaggga catgatcagg tagccgacaa aacccagccc
    10861 cgccagggac gcctggatcc gccccagcca tgaatcactg gtgagcaagg gcaaggaagg
    10921 cgccgaggtt gttggtccaa ggccggaggt ggggccgacg ccctcgtcgg cccgtcgatc
    10981 tgactcgctc ggcccggaag ccaactcgct cggcccggaa tccaactcgc tcggcccgga
    11041 atccaactcg ctcggcccgg aatcctttcc gggccctatc ttttcgctcc gcccggaatc
    11101 ccttccgggc gtcttcagct cctgcggcgg catcccccgc ctggcagccg cgaaaagctc
    11161 cccatgggcc ggctgcggag tctcgcccgc cttggccacc ggcaggacca gctcccactc
    11221 caccgccatc ggcgggaaac aaaccttggt ctgatccgaa caggcctgat acgtcgccac
    11281 cccgcgaatc cggacctcgc tgcctgtcaa tcccggctca gcttcaaccg gcaggagcac
    11341 gaacacccgc ccgcgatata ccgacagttg gtcacccggc aggccgctgg actcgacctt
    11401 gccggccgga aagcgagctc ggcctatgta caggccctct gtcttttcat ggaatacatc
    11461 ggtaggcatc aagaactcgc ccagcggcct gtgggagttg atatgatgac cctcagccac
    11521 gtcaaacacg accgccacct cgaacttggt gccgggcttt gctttgtgcg tgtcggcaac
    11581 ggcctccagc cgcttcaggt atttcgcttt ctccgctggc tggggaagat agctgcgagc
    11641 gagctggaac gtgtcctcgt tggcaggctc agctttcact tccccgccgg cgaccggcaa
    11701 ggtgatggcc agtttcttgt tacccagaaa gcacgccttg cggcacgcca tccaagtcac
    11761 gtccacgccg acggtgacct gcgacccggc tacggcctct ctgggcacct ctatgggcgc
    11821 gaggatcgtc ggctcgccct cgagaataaa ggtatgttcg ccacccgcgt cgacgtaccg
    11881 ctttggaaac ggataagcca acatgccgac ccgccagccc cccggtacct cccatttgaa
    11941 agaaggttca aggccgcctt caccggcgtt ccgccagtaa atgtgccacc catcctcaac
    12001 cttgaaccga agggccacat tgaccgtctc tcccggcacc caggctgtct gttccgagag
    12061 aagctccacc gctaccgacg gtgccttctc cgaagccatt agcccgcttg gggccaccgc
    12121 ccacgccaac aacaccatcg cccacataag cagatgtccg agcttcatac gtctcgcccc
    12181 cagaccccct gcatacgcat ggagagcttc tttatgcgag attgtagccg cttgcgtccg
    12241 tgacgcgata gagatacgag aaaacggatc gcgggcgaga aatgcgactc gaggctggag
    12301 accacggccg catcaaaaga agcaagccag aatcctggtg cgctgttgcc ctgtgtgtgt
    12361 aggctgccgc tctgcggcgg ttggttgtcc ctcgttcgca cccagctcgg ccatggccgc
    12421 tccccgcgcg cttacacgcc cgggcctatt ggatggagca gcttagagcc attggcgacc
    12481 gctccccgcg cgcttacatt ggactgctcg cggtcattcg cttggtggtg gacaagccag
    12541 tggcgttcga ggcaagacct tcagaggcaa tagacaaaaa cacggagccg gtgagagcca
    12601 ccggctccgt aacaaacaaa tcgaaaccct taactgtccg tcactccacc cgtcttaccg
    12661 acggcgacga agcatcggaa caccagccag cagcaacagg gcgctaaccg gctctggcac
    12721 tacccgcagt ccaaccacgt tgacatccga atgctctaca aagtccgtgt cgccgacata
    12781 ggcacccgtc actgtgatct cacccattaa agcggcggga tccatcggag cccgaacctt
    12841 catctcgaat actggccccg cagccgtaat gccgaagaca tcgccgaaaa cgttgaagat
    12901 cggacctgcc ggaaaagacc tcggccaatt gctaccctgc caagcggtat ttccatcggt
    12961 ggcgctccaa ccttgggcta cgccgggggc gtaagcggct acaccgggcg ctcccggtat
    13021 cttataaccg tctggtgcct ctatgaccac accccaggaa accaactcca tcccctgcgg
    13081 gtccagatta atctgcacca tgaattcttc tcccggtgcc acgattttga ccggcgagcc
    13141 accggaagta accgaaaagg tcgcaccata tccaatgccc gccatcatca ggacggccac
    13201 aaccactgtc attctcttca tattgatcct ccaatcctct cttctctcca gagaacccta
    13261 aaactaaaac cttcaatctt ccctccgctg ggtcctacat aacccttgcg gccaaatctt
    13321 cttcgctggg gcccccgccg gggtgtctgt ccaccccggc ggcaagaagc aactaatcat
    13381 ctcatcacgg gcacgtgtaa gtggggagcc cgaccttgct tttcgccagc gccacgtcgg
    13441 tagcagaaat cgtgccgtcg cagttcaggt cgcatcggac gttagcggca acactctggc
    13501 cgaccctaga cttcaccaat gccacgtcgg tcgcgctgac tctcttatct tggttagcgt
    13561 cacccgcaag agccaacaca ctgcacgtcg tatcgcctac cgtgcactgc gtggcacctg
    13621 ctacgtttat cgtataacag gtcttgtcta cattgcccgt aaccgtcagg atgagcgtat
    13681 cattggcaac cgcagtcgtg gcaagcgact cagccgtcac gaccagacca cccgtaatgc
    13741 tcacgccgcc cgcagcatag ttggctttgg tcagatcacc actgaactga atggagatct
    13801 tttgtatgcc gttattgcgc ggttccactg tagcggtccc atccgctgga ttgagtacaa
    13861 ttgcttgctg accagcagcg ccgtgtgttc tgaggctgtt ccacgcgatc ggactcactt
    13921 cttggcacac cggaacatac ccttgccact cgaacacaac ttcagcaggc gcgatcgctt
    13981 cggtgaccgc ctcggcaaag ccgccggccg tacccatata gtcggctggg atcacgttgt
    14041 tgctgttggc cggaaccgtg agcaccagct tgtaagtgcc gttttccaga ttcgacatgt
    14101 caatctggcc ttcggccaca ggcagggcac ccaggccaac gtacccatcc gggttccaga
    14161 caaccatggg tccggggttt tcagggcttt gcttggccaa gccgacaccc gcgctgttat
    14221 tggctgtcga gatcgaaaac gcgctgtacc caatacccat accgctgagc ttgctgggat
    14281 ccagcgtctc ggcaccgctc agttcgactc cgagattctc gcaaatgtcc agaatcccgt
    14341 tactgtcggc gtcggtcgtg ccattctctt cggtatcatc gttaatcagg ttcccgttgc
    14401 agtcggggaa gtaagcaggg gccttcatgc ggccgccgcc atgatacttc gtgctcggga
    14461 attgtacacg atccatgaac gcaccgccgt cggccggtac gtcatacaga cggccaggac
    14521 cgttcggtgg gccatattcc acagtgaaat acccgtcata ctccggatcc acgttccacc
    14581 ccaggcagaa tgcagcccgc tcgaaggggt tctggccggt caatggcggt ggcaactcac
    14641 taccatcgtt catcgtgctg aagaacttct tcggggccgc aggaacccag ttctcgccat
    14701 cctgtttgaa caactgaatg tcgaaaacca tgtttgcgat gcccccgatc atgtattgct
    14761 ccgtttgatc caggcccgct gccgccgcga cgacgtccca ggtcacaatg cctgtctgcg
    14821 gggccggagt cgtcgacccc gggacgaacg ccttatagtc gcccgcatga acgtcgtcgg
    14881 cgtagtttgt acccccgatc tcgatcgtgt aatcgatctc ggaccactgt gtttgcgccg
    14941 tcgctatgcc accaagcatc aaaacgatgg ccccgattgc agttactctc atggttcctg
    15001 cctcctcttg aattgcgtcg ggccttagag ctttaccaga actccggcga gcccgactaa
    15061 cacctcgaaa caccagaaac gctttctcaa agcatgcagg ccacatgcac gcctggggct
    15121 cctcctgccg cagaccaagc cataacctgc cgcttctacg cttcgcacac cgcctatcct
    15181 accgaacgcg gtcatcgatg tccagggaaa tccttgatga gctctgcgct cctcttccct
    15241 tccacctctc ccgcccgcca ccggacgagc cactcactgt cgacatcaaa ttgtacccaa
    15301 cgctcaggag actgcaagac cttttcgcgc aaattctctg caaattctcc cgacacattc
    15361 ccccgttcat gcgcggcgag cttcgctctg ccgggtcttg ttctctcgct ttcgttcaac
    15421 ttggccttgc tctcgaaaca acatcgcaag gctttgcctt gaaaaagctt atgcgctgat
    15481 cagcctagcc tcagcttcaa tgcctgcctt gccccagaat acccattatc cctatttatc
    15541 caaacctgca tggcgatgat gttcgcaggc tgcgccccat ctgaagccac gctggcacgg
    15601 caggaatgtc catctttccg ggaggctgcc tcgaagcctg atcccccttg aaacggccta
    15661 gctaacgaac tggccggctc gtacggggcg acattatggg gcctgtcctc agggccatac
    15721 caagcgcccg agcagcgccg gtcggcgggc gcctgtcacc tggggcaggt tacccggcac
    15781 gccgtcgata tacgcctttg ccaacatggc aaagctcacg gcctccttcg cctgcgcgga
    15841 aatcccgaat tcctcgattc gccggacagt cgctccccgc agctcagcct gcaacatgag
    15901 catcagcgtt gggttcctgg cacctccccc gcagacgatc acctccatgg caggcagtgc
    15961 tgagccaccg cgaatcttcc gccgccttgc cgataaagtc gccacgtcgg gcaggaaccg
    16021 gcgataggct tgggcaatcg agcgggcggt cagcgccgtg gctgtggcta cccagtcgtc
    16081 aggacgggag ccggcagcgg ccaaatgctt gatttctcgc gtcataaatg gcttgccaaa
    16141 atcctcgcgc ccagtggttt ttggaggttt aatttcaaaa taaggatgtc tcaaccactg
    16201 atccagtacg tcgcccagaa cccttccctt tgccgcccgc cgcccgccgc ggtccatcgt
    16261 ttctcggccg cccgtaatgc agcggaccac agcgtcaatg accatattcc ccggaccggt
    16321 atcgaaggcg ataacccgag aggcatcccc tccggccggc agccaagtta cattcgcgat
    16381 ccccccgatg ttctgtatcg ctcgcgagat tctgtgatgg cgaaagagcg cccagtcggt
    16441 ccacggcacc aacggagcac cctgcccccc tgctgccacg tccgattgcc tgaactcggc
    16501 aaccaccggc aaaccggtcc gcgctgccac cctggccggc tcacccaatt gaagagtaac
    16561 ggtccgaccc gtcggccccg gtagatggca gacggtctgg ccagacaggc cgatcaaaga
    16621 gggacgctcg ctgccacgag ccagaccgat ggcccatgcg gcagccttgc caaacgcctc
    16681 acccaagtcg gcgtgaagat gggcaatctc ctgcgtcgtg gtttcgccag gggccatcac
    16741 cgccaacagc cttcgccgca ggctgggcgg aaaaccggtg tgccgatgcc acaagagccg
    16801 aggccgcatc cgggctccaa ccccctcgat ccggacgcaa gccaggtcga tcccatcacc
    16861 actggtgccc gccatgagtc cgagaaccaa ccctacagcc gagcgatcgg caggcctccg
    16921 aagtctggag gatcgcttcg agatccgtgc gccgggcttg ttcattggcg atccgatccc
    16981 tgccattagc aaatgcggga acgtcccgcg gccgcaacat ccgtcacgtg cacaccctgt
    17041 cccccgcaac tgattgtgct atactgtggg ggtaatcgcg gagcgccgca aggtagatgc
    17101 cgaaatcgat tggccgtgcg gtaaccctcg gcggccgaca agttgaagga aagaacgaaa
    17161 gcgaaaaccg tctgttgatt cgcccgatgt tgacggccga tgaccaagaa aggtctcacc
    17221 tcaggcagga cctgctcggc tgatcgatgg gcaaccgagt cggtggcggc gtggattcgg
    17281 taaagtgacc caggagggtg tgtgcatggc aggggcagag tccaacgctg ttcgcccgcc
    17341 aatggctgtc tttcggaccg tcttgaccat tttcttcctc ctccccgcga ccttcgtcag
    17401 ggccgactcg agatggaagc tcatctatta ccgtcagtac ggcaatcagc tcaacgcgaa
    17461 caaccggacc atcgaaaacc actgtttcaa cgacaacggc accaaggcgg cgggtatcgg
    17521 cgtgaccaac ctgtcgaact cgggatgggc catcttcggt accaccgact tcaccggatg
    17581 gaatcgcctt aacccgctcg atcccaacaa ctgcgcctac gacatccgga tctacgaccc
    17641 gagcacaccc acggaccaga gtccgaactt caactggcag aacacacagc caccctggcc
    17701 cgattactac agctacataa cgtggtggat gaaggtctcg gacgacaccc gcgtgcgggc
    17761 ttttccgcaa aagccggtgt atcactacga tcttgtagac ggcctcaatc aggccggcgg
    17821 ctttaccggc ggttgcatcc caaccggttg ggtcgaggcg ccaggtgcca acagcgacta
    17881 tcgcgagctt cccaccggcg gctgggcgac ctaccaggct caaaccttcg tcgtgcctgc
    17941 aggcattaat cgtatcgttg cggcatcagc gttcctgacc cgcgggagct ccccgccggg
    18001 gccgaagttc acataccggg ccacgatccg agagggcagc ccgacgggca cgcagatcgg
    18061 tccggcaacc gtctcgcgcg aggtcttctc aggcgaattc aaagaggttg cggtctgctg
    18121 gccactcaac ggtgtgccgg ttaccgaagg acagacctat gcccttcacc ttgaggccac
    18181 cgacggacaa ggcttcaacg tctatcgcac cgtcaataac aactacccga acggctcact
    18241 ctggcacggc gatactcacg tggccgagca tgacatggtg gccgtcgtgg tcggcgtcgg
    18301 gtacgacgcc tccccgcccc atatcagtcg aaatcctgcc ctcctgacaa agtcggtcgt
    18361 acgaaagaac aacctgccga acgacacctt caccattgct aatggcggag gcggcacact
    18421 gaactacacc atcaacgaca atgcgagctg gttgtcggtc gaccccgcag ccggctcgtc
    18481 aaccggtgag acggatacga tcagtatact ctacggcacg gccggtctgg caatcggttc
    18541 ctatagcggt acgatcacca tcaatgccac gggggcggac aacacaccgc aaacggtcgt
    18601 tgtgaatctg tcggtgaccg ctcctccatt tgcgccttgc gatttcgaca ctgaccacga
    18661 cgtcgatcag gaggatttcg gagtgttcca gagctgttac accgggcccg gcgtgaatca
    18721 gacggacccg gcttgctccg gggcgctgct ggatctggac acggatgtcg actatgagga
    18781 cttcgccaga ttccttgcct gcatgggcgg cccggacatt ccggtggaca ccacctgtgc
    18841 ggactgagcg agggcccaaa cgttgcgtga tgcgaaaccg ggtgagtgag ggttggtgag
    18901 cgagttctgt tcagatagga gggtcataat gaatacctgc cagccacctc gttggctgaa
    18961 tgctgggttg atgcggcttg tctgcgttgg cctactcatg ccggcgaccg ttgccgtcgc
    19021 tgatggaacc gtgagcggac agtgccggtt cgagcagatc aaaggaagaa gcatctggta
    19081 cacgaacctg cacgaatgga atgccttttt gatccccagt tggtcgatgt tcgcagatcc
    19141 gctgagctac cgcgtggacc acggcaacta cactttcacg aaccccgccg gcgactacac
    19201 cctctacgtc aatcagccgg aattcttcgg ccgggctaaa gtcgtccgag atgtacagat
    19261 catcaacggc cagacggtca cggttcatcc ctcactcaac ctcgattact cctgctacta
    19321 caaaaacgag tggccgagcg gctggggaac cccctggtac cagactttca tcgctacggg
    19381 cacgtcggtc aacaagatcg tctactcctg tgcgagcggc gatacgggca cgatcaacgt
    19441 cagtctgctg aaggacaccg gaggcaacgt tacaacctgg acccaggtgg gagtgaccaa
    19501 gtcttcggga agtagcatcg gcgagtcgtg ggtcggctac ctcagcgggc aactcccgac
    19561 tgtcccgggc gagcgatacg ccatccgcct cgcgggagaa gcaagcacac ccttttctcc
    19621 ctactggcgc accgacaacg gcgacggcta tgccggcggc caggcgtttg accccaacgg
    19681 aaacccgctg aacaaagacc tgtgcatcct cgttttcagc gacaacgacg gcaccgtcat
    19741 cccctacatg aagacgaccg ggggaatggg ctcaacggcg tggtgggaat ggcaatgggg
    19801 ccagacgttc aaggccaccg gcgaagcgct ggcgggtgtc gatctctgga tctctcacgg
    19861 cggcgacctg cagtggaaca tgcggacgga ggttaaggtt tactccaatc cacccacggg
    19921 caacccgccc acgcccagcg ggctggtggg tttggtgaaa cgcggacgcg gcgcctggca
    19981 ggccggcggc gccgccatgc tcggcatcgg ctacgcgccg ggtgaggtgc ctctcgtggc
    20041 cggcaacaca tactacatcg agatcacacc gtacggcggc gacgcgatcg gctacgaggc
    20101 ttaccggttc gatcagccgg cggacgccta cccctacggg cacgcctact caaaccgcaa
    20161 cccgcaaccc gacatcgacc tgagcatgac catcatggag tacgccgtca gcggtccgcc
    20221 cccgcagatc gagcgcaacc cagcctcgtt cacccgtagc gtcgagcgag gaaagaatct
    20281 gcctgacgct accttcaccg tccgtaacgc cggcggcggc gtaatgcctt acacgatcag
    20341 cgacgacgcc gcgtggctgt cggtcgctcc caacagcggt acgtcgtcgg gggaagttga
    20401 tcccatcggt attctcttca acacgtcgac gcttgcggtc ggttcataca ccggcacgat
    20461 caccatcagc gcgccgaatg ccgccaaccc gactgagacg gtcatcgtct atctgacggt
    20521 cacaccgccc ctgtttgctc cctgtgactt taacaccgac catgatgtcg accagacgga
    20581 tttcggtgtc tttcaggctt gctacagcgg agcgggggtc acacaaaccg acgtcgcctg
    20641 caccggagcc gacctggatg gcgacggcga cgtcgacatg gatgacttca cgcgatttgt
    20701 cgggtgcctc tccggtccgg gtgtgcaagc cgatacgctc tgtgcggggt gagcgattaa
    20761 gaccctgtcc tcggttggtg tgggaccggc gcttccggct ttgtggggat ctcgccgcgg
    20821 ccaagaccgt aacgaacaag ttcaccgttg tgcccgcccg gatctgtgcc cacgatgtcg
    20881 gcagtcgcga gcttctgcct tttcccgccc gagcagcctt cgggacctcg ggcaagatta
    20941 ggcgcaccga tttcggcaca agctgttcac ttacgataca cccctcttga tataagcatg
    21001 atcgcttcct tacagggttt cacgaccgga gcggttgctg tcaaatttct gcaaagtatt
    21061 tgaacgatcc ggccgctacc gacggtataa tcctttgcat gctgacgcga cttgccatga
    21121 tcggtttgct actcacccaa ccgctacgag tctgcttcgc gggcgcgtgc gagtcggtct
    21181 gccgatcaga ggagcagccg acgacatgct gcaaaggcac gtgcgcgggg gaggcaaagc
    21241 cgcagtgttg ccggacggca tccgagacga cggagccgtc atgcccctgc accttctgcc
    21301 caaacgggcc gcgcgagccg gtcaaccgac cgaataaccc cccttcggta aacgatcagc
    21361 catccatcgc agccgtcacc tggatctgtg ccgatggcga cgacaaaccg agcttgtcgt
    21421 tgctaacagc caaccgggcc ggctcttgtc gaagcgtgtc cggcaatacc cgccaagcct
    21481 ctctttgtgt ctggctcaac tgagcaagcc atcctgggaa tactgtgcgc ccggaaaacc
    21541 cctgacgggc ttcattcgtg tatcgacggg gcctggccgt cctgcatgga cggaacggcc
    21601 agggcaggtt ttggacaagc acaagtatgt gaattgatct cttccaggaa gacaggagtc
    21661 tgaacatgaa acattttgcg attactcttg tggctgtatg tgcgttggtg ttggctgcct
    21721 ttgccgccag gccgcttctg gcaggcccac cagtgccggc cgccggcatg agctgcggtg
    21781 acaattgcgg caccgacagg tacgaaacca agtgcggcga caactgcaaa tgtggcgaca
    21841 attgccagtg cggtaacgac tgcaaatgcg gcgatgagtg caagtgtggc gacgactgca
    21901 aatgcggcga caactgcaag tgcaaatgca aggacaagtc tgaggacaag tccaagggca
    21961 agtgcaaaga caagtgcagg gacaaaaagt gacaggcggc tcgccgtgaa tcggggataa
    22021 tccacggcaa ccgcccggcg gttaaatacc cgccggccgc caacgacagg ccgggtccat
    22081 caaccgatgg atccggcctt ctttgtgtcg ccatccgcac caagcgagaa tgcggtgcgc
    22141 gacgattcag gcagggctgc cgcaagaccg attcagaacc cgcagcgtaa gcgacggccc
    22201 cgaccggatg gcgaagctgc ttggtcagcg agggtccgcg ccgacagccc ttcacgcgtg
    22261 caacgcggcg caggctgaaa tatactgact gcgcggcatg gtccgggcac atgggcacgc
    22321 aggactgcgt cacacttgaa cggattgtcg gcatttgcct gaaccccgct tgccacggcg
    22381 tagctcgcag agcgaagtcg ggaacactgc ttgccgcggc gtagctcaaa aagcgaagcc
    22441 gggaaccctt atggaagtgc gtctcgcgag aaaacacggt ttctgcttcg gcgtcgagca
    22501 ggcgatcgag ctcgccgagc aaaccttacg cgacaatccg cctggccgcg tcgcggccct
    22561 gggcccggtg atccacaacc cccaagtcgt ccgaagactt gaacaggaag gcctcaatca
    22621 gtccgcccac ctcgacaacc tggaggccgg tacaaccgtg ctgatccgtt cgcacggcgt
    22681 cgggccggag atctatgaaa tggcccgtca gcgcggcatc aacctcgtcg atgcgacgtg
    22741 cagactcgtc aagcgcgccc agaccgtggt caaacaactg cacgaggaag gctatcaggt
    22801 ggtcatgatc ggcgacgcca accacccgga ggtcaaaggg gttatcggct acgcccccaa
    22861 cgtcatcgtg gtcgagcaag aggaagatct ggcgaacaag atcccgccac gggcgcggct
    22921 cggcattgtc gcacagacca ctcacgcccc cgagcacgtc gcccgaatga tcggagcaat
    22981 tgccgccctg ccgttccagg aactcagagt cgtcaacacg ctctgtctgg aggtcgttcg
    23041 acgccaggag gcagccgtcg aactcgcccg cgaggtcgac gtcatgttcg tgcttggcgg
    23101 cctgacctcg gccaacacct gcgaactggc ccggctctgt caaggcgaag gcgtccgaac
    23161 gtatcatctg gaaacctggg ctgacttcag acccgaaatg gtcgccggct gcaaggtcgc
    23221 cggcatcacc gccggcgcct ccacgcccga cgacacgatc accgaattcg ccgagaacct
    23281 tcggcgctat aacccgatca agcagcccga ttagcagctc gcctgctttg ccgcatcatc
    23341 gcagctttcc ttcgaagctt gatcagccga ccttgccggt tgtccttcgg cctcattcgc
    23401 agggggacga cggcaacaac cggggccgcg acacaagcca gggtcgcgaa atcggcgcgc
    23461 ccgcgcaacc ccgccctcag ccgtcgcagc cggatggcac cacaaggccc ggacccgtgg
    23521 cacaggcctc gaacgcggcc aggtcgttcg catccacgtc gttgtcaccc agggggaagc
    23581 cccactcggg ccgatcaaag caaacgcacc cggccagtgc cggggtcaga tccccggaga
    23641 cgcagatctg catgatcgcg aaatccgcct ggtcgacgtc cccgtcgcca tccgcgtccg
    23701 cgaagggatc gcggcagtcg ggcgagacca ccgaaacgta agcgacggag tcgtcgaggt
    23761 tgatccatcc gacgttttct ccccaggcgt agccgcggaa acgcccggcc caataattgc
    23821 ccgcgggggc atattcgaag cgggcctgcc ggccgtaagt gccgagggcg gcccaggtat
    23881 cgaaattgac ccagccgatg ttctcgcccc agcccagacc cgacaagtcg ccggtcaacg
    23941 catcgatgtt gacgccgcaa tcctcgccgt tggtgttggc gtacgccgtg ccgttggccg
    24001 gccgaccgtt acccaggttg atccagccaa cgttttccgc ccagacgaaa cccgaaagga
    24061 acgagggatg cacccgaacg ttcgaattgg ccccgtgcca gttaagccaa ccgacgttct
    24121 cggcccacgc aaatcggtga ttcggatcga tgttggactc cccggcggcc aatacacaga
    24181 cggtgcccgc acagagtagc gccgctgcaa gacgatgaac catatctcac ctcctgccgt
    24241 caccggctcc cttaatggaa ccggtgatcg cgcggctcag ttgtactcga tgatctggta
    24301 actcacccgg cggatcccgg tcaacggtac ttcgtcaacg cgaatcgtga gcgacgaagc
    24361 cgtcagatcc atgaggcaag cagcggtgcg acccgcaagc gtgttcctcg cgtctgacat
    24421 atttacaacg acggcgtcgc tgagaacgac catgcttttg gagggcatga tctccggcgt
    24481 gaagttggca ctgccctcga ccgcgccact ggccagttcg atgacgcccc ggacaacctg
    24541 tttcactcct cctcctccgc cgccggggat tccggcgatg gccgacgcca actgagcgtg
    24601 ctccgccgaa agctgctcga gcgttttcat cgtgggggat accgggccca cgggcggatt
    24661 aagatctccg gcttggaccc aaagaacgag acacaagcag accaccatcc ctgtcacgat
    24721 gagtccgcga atgcgacgcg atgacataga agcctccttc caaacgcatt gccacttttc
    24781 caatcagacg aaaatgtcct ccggactgat tcacaatccc tcaggaccgc ccgtagcacc
    24841 gtgccacctg aatcagcttt tggcgggtca tcaacctacc aaagatcctt aagacgtgca
    24901 acataaaaag agggataggc ctatgctact gaaggataag agatacccct atggtatggc
    24961 agaaatgaac tgaggtcgga ctggggcgat gctcatccgg cgtcggggaa accaatctgg
    25021 cagccccctc caacgacgtt tttgggcctc cgccggcttt tcttggaagc aagggctgaa
    25081 cccaaagaaa aacgcccggc gcgtcgcaac acacaccggg cgtttcgtat atcaagttaa
    25141 cagtgtgacg ccgggcgggg aattgctccc accgctcacc cactatgggc cgagaacgcg
    25201 ctgactcatg gcacgccttg tcggaccggc ggcccaagcc gaccgatccg ccgcgaacca
    25261 cgtctagtga tccactagaa aggaggtgat ccagccgcag gttcccctac ggctaccttg
    25321 ttacgactta gtcccagtca ccggtctcac cgtcggccgc cgcctcctcg attaaaaggt
    25381 tagctaagcg gacttcgggt gctaccagct cccatgactt gacgggcggt gtgtacaagg
    25441 ctcaggaaca cattcaccgc gtcgtagctg atacgcgatt actagcgatt ccgacttcat
    25501 ggagtcgaat tgcagactcc aatccgaact ggggcgcgtt ttttgcgatt tgctccacct
    25561 cacggtcttg cgtcgctttg tacgcgccat tgtagcacgt gtgcgtcccc gggcataaag
    25621 gccatgatga cttgacgtca tccccacctt cctccggctt aacgccggca gtctcgctag
    25681 agtccccgcc attacgcgct ggcaactagc gacaggggtt tcgctcgtta agggacttaa
    25741 cccgacactt cacagcacga gctgacgaca gccatgcaac acctgtgcaa gtttcacccc
    25801 gaagggcagc ccaaccgagt tatccccggc gggcatccaa gcatgtcaaa cccgggtaag
    25861 gttcttcgcg ttgcttcgaa ttaagccaca tgctccaccg cttgtgtgag cccccgtcaa
    25921 ttcctttgag ttt
//
LOCUS       DAOTPW010000051        25882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1974_length_25882_cov_5.916250, whole genome shotgun sequence.
ACCESSION   DAOTPW010000051 DAOTPW010000000
VERSION     DAOTPW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 25882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25882
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1974_length_25882_cov_5.916250"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="P5316_06590"
     CDS             complement(<1..325)
                     /locus_tag="P5316_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84661.1"
                     /translation="MLKNGDNATDRNCTLATTRPAPQAGLRANPQVSPAAASQGLDLS
                     RPIHDAGGIAGLLAVEEETQGTFHGTYWFFYDGNGNVGQLVKTIKDGQGNITGVENTL
                     AAHYEY"
     gene            complement(877..1374)
                     /locus_tag="P5316_06595"
     CDS             complement(877..1374)
                     /locus_tag="P5316_06595"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRR84662.1"
                     /translation="MNVVLVMFKDDGSSKEFPLSPGKTLVGRSEECDLRIPLPEISRK
                     HSVLMVTGNTVAVRDLGSANGTYVNNKRISEQELAAGDHLVIGPVVFTVKVNGEPKNI
                     KPVHTRLETRRPATAKQPATPKPATAGATAKGEEDPISALEALAGTDDTAEMDLDGSS
                     IFGDE"
     gene            complement(2755..4617)
                     /locus_tag="P5316_06600"
     CDS             complement(2755..4617)
                     /locus_tag="P5316_06600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRR84663.1"
                     /translation="MDSLLEVVKKHWGYLSFRPLQHEAMVAAVEGRDSLVVLPTGGGK
                     SLCYQAPALLSDKLTVVVSPLIALMKDQVDRLIQRGISSAFFNSSLDAADRRRVMAGI
                     TRREYKLLFVAPERFADPLFVDMLGHVGAGSFAIDEAHCISHWGHDFRADYRRLGQLK
                     VRFPNAAVHGFTATATPTVRDDIIAQLGLRKPVVLVGDFFRPNLRYRVVRRTSTLANV
                     LSVIKQHRGHVGIVYCIRRDDVDGLTAQLQRAGVRAIGYHAGMSDEVRTQAQDDFSAG
                     KADVVVATVAFGMGIDRADIRYVVHAAMPKSIEHYQQETGRAGRDGLPADCVLFYTGG
                     DFHLWRTIIRKNESADLENKLRMLSEMYDFCTGISCRHQRLVSYFGQAWDRASCDACD
                     VCTGTLERAPDSTVTAQKILSCIVRTGERYGAAYVADVLMGVPTDRIVDRGHDKLSTF
                     GLITDRPKGVLIMWMHQLADQGFLRIDGEYRVFKMTPEGWKVLRSQAVARLSDPGRRD
                     GRQRRERRTDDPDRPKRAPTRTTRLPPLSGKSEPLDAAAMNLLGKLRDLRRHLADELN
                     VPAFMIFSDKTLREMARRRPRSPDEFLDIKGVGAIKSRQFGERFLEAIQSHLRQ"
     gene            complement(4668..4868)
                     /locus_tag="P5316_06605"
     CDS             complement(4668..4868)
                     /locus_tag="P5316_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84664.1"
                     /translation="MFSPFRGRFESWTAIRARMLRETEVFIEEGLRHPERQVRIPALK
                     VGSGTFTRIFAQAFWAQVLGTS"
     gene            complement(4868..5803)
                     /locus_tag="P5316_06610"
     CDS             complement(4868..5803)
                     /locus_tag="P5316_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84665.1"
                     /translation="MESAVTIAPPHVGLIDRTRVALACGVAILMMYGVGWNVAQPPDS
                     LLGLTFTAGGRSSYFHIWLMMLVLGAATSAIATAVAGKRLPEAGLFATGVGMATMALR
                     GGSMQSVLAYCASPETASRRELMRLMGQDCLLWAAVMALAWAASTLTRKWLWPDSKVS
                     ACPARTKPVSPQLGAATLASTLIVALIVIWLTIARTPVAEIARGQVIASVAAGLFLGA
                     MAGRTVTGVDNVRWYLPAVVLVGLTAYLIGYLESDMSWAGSGSYRPYAALATTPPHDL
                     VRPLPIEYMTVGVAAVLGGFWCGNKVEQTVEEGTR"
     gene            complement(5952..8627)
                     /locus_tag="P5316_06615"
     CDS             complement(5952..8627)
                     /locus_tag="P5316_06615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRR84666.1"
                     /translation="MELTATARKLPSLRDRLSRLNYTKACQLLGENGKELIKLGGAYE
                     IRLDEQVYLGEDLFRLSLPDAVVTITAMAEAKNRLRFNCTSCNTVCRHIGAALALIIE
                     EKMSLGLAAPPRERVPVESLSEEKLIEQALEERRERARTGNMTVTSMDPARLWTDYLV
                     TNRMSGKTYRVALRGWERGQSYCSCPDYRKNTLGTCKHIMRVAEKVKKRFPENTRLRP
                     YVPKDLAVYLQYGEQLELRLLTPPNLNGAAGALIGPVSGRAIQDIPDLLRRIQKLAIL
                     GYPVVIYPDAEEYINQQLFLQRMAAKTAEIRKNPKDHPLRKTLLKAELLPYQMDGIAF
                     AVGAGRAILADDMGLGKTIQGIGVAELLAREVNIRKVLIVCPTSLKSQWRSEIHRFSN
                     RDCQLVLGGAAERAAQYDNDRFFTICNYEQVMRDILSVEKVKWDLIILDEGQRIKNWE
                     TKTSRVIKGLKSPFALVLSGTPLENRLDDLFSVAEFIDDRRLGPAFRFFNRHRITDKT
                     GYVLGYKNLDELRARLKPVLLRRTRDSVMKQLPPRTTEIVRITPTDEQLKLHQTYSQI
                     VRSIVTKPYITEMDLLRLRQALTLCRMTADSTYLATKEEPGYSSKLETLDNLLEQLVA
                     EENRKILLFSEWTTMLNLIEPLLNKRKIAFVRLDGSIPQKKRQPLVNAFQNDPRIKVF
                     ITTNAGATGLNLQAANTVINVDLPWNPAVLEQRISRAHRMGQEQPVQVFVLVTEATIE
                     ENLLGTLSAKHDLAMAALDAESDVSQVDLMTGIDELKRRLEVLIGARPYAPVDESEKR
                     RVMEEAERLARADRVGSAGGRLLTAALGFMSELLPERPQTPEFDDMVETLRARLSECI
                     TQDEKGQHKLTVTLGDDSAVNNLLHALARLLSLKG"
     gene            complement(8706..9701)
                     /locus_tag="P5316_06620"
     CDS             complement(8706..9701)
                     /locus_tag="P5316_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRR84667.1"
                     /translation="MSKQWTNEQILDLARAYQPACVIAAAADLDLFTVIADQEMAADT
                     LAGRLQVDTRALTVLLDALASIGLLNKRHGKYRTPAEVSAALSQDGPRSVLPMVQHTA
                     NCMRRWAHLARVVKTGRPAERTPSIRGEAADQAAFIGAMHVVSEPVADRVVSDIHPPP
                     FKHLLDIGGASGTWTMAFLRLDPQATATIFDLPEVIPMAEQRLREAGFAERVRLAPGD
                     FYVDKLPAGADLVWLSAIAHQNSRRQNRELFAGIHDALIPGGTLLLRDVVMNDDHTAP
                     PGGAMFAVNMLVNTEGGGTYSLSEYRQDLEASGFKDVRLLREDPWMNSVIRASRN"
     gene            complement(9739..11613)
                     /locus_tag="P5316_06625"
     CDS             complement(9739..11613)
                     /locus_tag="P5316_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84668.1"
                     /translation="MSAVDLAILIAYIGAVVTIGFWVKRRASKGLESYFLGGRELPWW
                     MIAMSGSSSYFDITGTMWIVSMFVAYGFNGFWIQWMWGFLIAAFYLAYMGKWIRRSGV
                     ITGSEWMVYRFGPTRSGDTARLAYTTFAVLTLTTFIGYTAVGMGKFGGQFLPIREWVP
                     ILADWTPRNIDRLCAVLIISLTGAYVVVGGFTGLTIVDFVQTIVLTTGAAMIAVLGFA
                     TFDEQPVAAALAKLAEGTNRIVQVNPVQWYSLLPPWQIENQADQYALFGAVVIAFLMK
                     GLLLGLSGPEQLYDFQKFLATRNTREASLAGMLWGICHTVRFPMAMAITIIGLVAVAG
                     STETGFDAEKVLPYVIAHKLPPVLKGLALAALIAAFMATFGAMVNGSASYLIRDIYQR
                     YVNPSASTRHYVRASYLASILMILIGIAISLVSSSIATMITWILGFLGSAVLVPNVLR
                     WYWWRISGYGFAAGMWTGMVFSLIQTFLDEPIAGYMGWGRSVPVYYVIPVLASVSAAA
                     CIIVTLLTRPPDMNTLAHFYRTTKPAGRWGPVVEYIRRTNPEFRKEPFLPDIAALVVG
                     LFWLGAMYVGPSYLVAHQWLPAGICALMVAVGSVVLAASWYRRLESGEPRPASPKAIP
                     "
     gene            12041..12244
                     /locus_tag="P5316_06630"
     CDS             12041..12244
                     /locus_tag="P5316_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84669.1"
                     /translation="MLISVCFGGTDGEAVEAEPDEGAGSWLPWNRGSLRLWSIFKWQG
                     TGQAFRMMAFPMPQKGMMTELHL"
     gene            12241..12471
                     /locus_tag="P5316_06635"
     CDS             12241..12471
                     /locus_tag="P5316_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRR84670.1"
                     /translation="MTRTILTIRGLHNKRSASNVARSLKATKGVTGVAIDVQSRSACV
                     EHDEQAKVEQLISAVKQVGLQVDGFKPSDAEA"
     gene            complement(12565..13662)
                     /locus_tag="P5316_06640"
     CDS             complement(12565..13662)
                     /locus_tag="P5316_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84671.1"
                     /translation="MNKVKVGIIGSGFVADLHAAAFKMIPEGEVVAVASPSPGKAKKF
                     AEERGIPKAFHDYHDLLAVKDIEMVNLCVPNDLHAEVAVAAAKAGKHVVCEKPLCRTL
                     DEADGMIAACRDAGVLLMYAEELCFAPKYVRAKTLVDEGALGKAFLVKQSEEHFGPHM
                     PWFWDVNRSGGGVLLDMGCHSIEFARWVFGKPAVKSVYAQMGTYVHADKTRGEDHSLC
                     IVEYEGGRVGLAENSWAKQGGVDDRCEIYGSGGFTRADLLRGSALLTYSQPGYGYAVE
                     KAATTKGYTFTMFEEIWNYGFPQEMAHFCRCVRGLEKPIETGEDGREVLKIILAAYHS
                     AGTGRRIEWPYQPPKTDKPIDLWRQIDTPKP"
     gene            complement(13699..14724)
                     /locus_tag="P5316_06645"
     CDS             complement(13699..14724)
                     /locus_tag="P5316_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84672.1"
                     /translation="MIRVGLIGAGFIGRNHFNQYEKMPERVTLIICDKEPDRREGDWS
                     KVGGNIADSKGSKRDVSHLQRYSDWKQLIADPNVDAVDICVPTPLHKEITLAALLSGK
                     HVLCEKPMALTVEDCDTMLAAAAKSKARFMVAQVIRFWPEYRFLRQIVENRRYGQLKA
                     LHLRRQAATPGYTLNNWVLNPKLSGGAVLDLHVHDVDFVLGLLGKPKAVCAQGHIKPN
                     GSCDRIHAQWYYDAVPLVQLEGWWDMPGNFPFNMGFTAVFENGGVHWDMNTGKPLAVY
                     ENGKDPYTPEIPTEDGYYAEIDYFLSCIEQNRDPKLTTPKESRDAVALALAEQQSAET
                     ARQVEIT"
     gene            15013..18282
                     /locus_tag="P5316_06650"
     CDS             15013..18282
                     /locus_tag="P5316_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRR84673.1"
                     /translation="MRNAIPNTIIVTGLFSITAFAADIPWPEHPRPDFQRSPWVNLNG
                     QWDFDFDPQDVGEKEQWFVPVQHAFSRKITVPFPWESKLSGIGDKDYKGVAWYSREIA
                     LPDGEGWKGKDAWLIVGACDWEARVWVNGQPAAEHVGGYLPFEVNLSQFAKPGEKVAI
                     VIRAKDVTDDQQPTGKQIGWYTRTSGIWQTVYVEPRPSRYINWIKAYPDIKTGEVRFA
                     VSCIGSGINSLLVTSPDNSFDTVAVSLSASGGDTSGEPRSAKIRVKEPRLWSPDSPHI
                     YPVAVEISSELASRSKDRVESYFALRDISVGPAPGRDYQYVCLNGKPIYLRGALHQSF
                     HPDGIYQYPNDAVMRSDYELCKKIGINFLRIHIKTPVPRELYWADKLGVMIMQDMPNF
                     WNNTGQARKWYEEMLHGAVARDFNHPAIFAWVDFNETWGLDNPGPYSEERQQWVENIY
                     KLTKLLDPTRLVEDNSPCKYDHVVTDINSWHFYINDYEQAKKHIQEVVDKTYPGSTFN
                     YIGGRRQADAPLMNSEYGGISAGHGDQDISWCFKYLTNELRKHDKICGYIYTELSDIE
                     WEHNGYVNYDRSPKEYGYDFWFPGFSLADINSPDFVVIDAPPVIELKDGQPREIPIKI
                     SHWSDKQAKALTLRRRVDWIDRDGVKWEGQERKTQPAIWKPYEVVDQPAINVSAEWGV
                     GPVVGALLVELVDGDDILARNYVNLLVRRSAPAAEVVGKACFLRFRPGDFSAWQWEED
                     PTTMPVAVSADKACIRWSGYIEYQIDTGIKSPDGLVGLDLTAEVSAKAGDEKLDWPER
                     KKDIDYPQTDVKKWPSDLRISINGVEIHRETLPDDPADARGVLSHHRQYHPGSYGFLV
                     TANVQDESIVKRIFDAAKDGVLTIRFEVPADAKNKGGLAIFGDALGCYPVMPTLVVYS
                     RDEMPQPPASFTARSTAAVNRFADRAKSLLPTAEGGGHTWRWTTDRPDDRWNQPDFDD
                     RQWKTGRSGFGKEGTPNTIIRTPWTSQDIWLRTEVTIEDPATIVAGQWRLYHDENCEV
                     FVNGKQVIRQRGFVTQYKDVPLGREALSALRPGRNIIAIHCRQTEGGQYIDAGLTVLV
                     K"
     gene            18449..19012
                     /locus_tag="P5316_06655"
     CDS             18449..19012
                     /locus_tag="P5316_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84674.1"
                     /translation="MIRPSLLLLPVLFWGCGSGTRSCLDPSRRSAEDPEAIRIKPHHF
                     VDIITSYGRGEEFKPHPYGHAVHTVAERILSNRDVMLVMELGADDICGPCKHNVGGVC
                     DDVIDTSYRPQAPSRKQEWNLLIDERWCKRLELLQGDRLTARQFCERLRDRAGDITDI
                     YREIPAEMTAERVKAMREGVRRFLQGP"
     gene            complement(19034..19318)
                     /locus_tag="P5316_06660"
     CDS             complement(19034..19318)
                     /locus_tag="P5316_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84675.1"
                     /translation="MRQGHDVSHNPHDNYWCLEPERTIDFMQTVNKPWIPYKILAAGA
                     IEPEAGFRYAFQNEADFACVGMFDFQVVQDVNILVGLWPDAQKRKRPWLA"
     gene            complement(19818..19891)
                     /locus_tag="P5316_06665"
     tRNA            complement(19818..19891)
                     /locus_tag="P5316_06665"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19855..19857),aa:Asp,seq:gtc)
     gene            complement(20129..20791)
                     /locus_tag="P5316_06670"
     CDS             complement(20129..20791)
                     /locus_tag="P5316_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84676.1"
                     /translation="MRTALAHADLFLRGSGPFLPEQAASRPGWWLPLMVVAFSPIYGA
                     FMGSFDLGSAERLLIIAYSGAKVPLLLFATTALCLPGFFVLNTVLGLRDDFREAVQAV
                     LAGQAGLSIALASLAPLTRFWYFSCGSYRAALLFNAAMFTVATAAGQIIMLRYYRPLV
                     RRNRRHRLMLMVWVGLYAFVGMQMGWMLRPFIGDPKAPVAFFRQEPFSNAYVVIARLI
                     FR"
     gene            complement(20810..21664)
                     /locus_tag="P5316_06675"
     CDS             complement(20810..21664)
                     /locus_tag="P5316_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84677.1"
                     /translation="MLSAFKQLDELLRGRITSPGRLAEGKVELSLRRFVPLAVGLGAS
                     YGFFMGWYALSIYWGGTSPEPQRCWQLLASMVKLPALFLLTLMVTFPSLYVFNAMVGC
                     RLSFTATLRLLVGAIAVNVAVAASLGPILGFFTVSTTSYPFMVLLNVAFLAVAGCVAL
                     AFLLNTLQRLAYPVVPPPAPTSPPPPDAPTDMGRSSLAEFSTAPPRELGPLDPQPSPM
                     PDYAIGQAKTIFRIWLLIYCLVGAQMGWVLRPFIGNPNMPFSWFRPRSGNFFQAVFHQ
                     IQALLGLN"
     gene            21962..24202
                     /locus_tag="P5316_06680"
     CDS             21962..24202
                     /locus_tag="P5316_06680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRR84678.1"
                     /translation="MPPDTTAQQRQALTERLLAAFDGSIPRVWKSPFYLLGLLIVATV
                     ILLLPLVYLGFIALTGYGIYYHIIHNIDVLSLPSAVHRRTVFVIMLLRLAAYFGPILI
                     GMILLLFMVKPLFARPARSERRLSFVRENEPILFAFVEKLCEVVRAPVPRRIDVDCDI
                     NAAAFFRRGFLSFLGRDLVLRIGAPLVAGLSLREFAGVLAHELGHFSQGTGMRLTYII
                     GCVNGWFARVVYQRDTWDDRLIALSQEQGWFSLFFLVARLFVWLTRRILWLLMIVAYA
                     MSCSLLRQMEYDADRYWARICGSECSESSLKRLHALAKASQTAFAFIAHCWQEHRLPE
                     DLPALIVAEAERLSPEARQEIEKGISESRTYMLATHPSDRIRIQRTRSENEPGIFHLA
                     EPASVLFVDFDALCREVTFTFYQGLIGPEVRREHLLPRSDVDQKRRLLDETLEAARRF
                     WTTLDFVTRPFRIDRFSPVFDLPPRECLERLKRARCALERSQHTIRKEYGRLRELDQT
                     ISNARQAECLLKAGFRVDPEMIGLKKADPLQAHEVGRRAERDRQGVEERLLKIEAVFA
                     VRLEAALALLKCDRVAARLENAGALRAKSEQLLDVTAGLYRAEYLLTSLRLNQNSLRA
                     MEDAISRGYEEDALIAQTLRLLETQNRALAELREATKEVPYPYEHAGGPVSVAQYAIG
                     IVPSVNDLTGTGAAVSACLDNLFALYVRVMGELARIGEQVEAVVGLQPLAVTKDAPAT
                     DRPGVQ"
     gene            24427..>25882
                     /locus_tag="P5316_06685"
     CDS             24427..>25882
                     /locus_tag="P5316_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84679.1"
                     /translation="MFTIHPAGASVGRLSSCLVVLPVLTITIGCTTGPNGLDVDRAWR
                     EAEVARRGACEPNADIACAWWPDLRDIWTPVGWKDHAFRFNVLFNGTILAQPDMNPRT
                     QKWKNQGVQLAFEPSYDGKWRNYGYIHRDDTMVRQCWNEAHAAPIVRSEWTQHGLSLR
                     QEVFGHVPGGKRLTSGTEPIFAWVRLSIASICPGVPAEKNHTFLIKLNAPHISAGMTY
                     MNNIQFHLDRSAYPRRLTAEPAGDGWRLLEEGGKVRLGVARGRPVEINFTPGKPTEKD
                     SLIALTMEVKPGNHVDVLVPMLPTEREVFDAELMLGLEGALAEADAFWAIVPATAAVV
                     DTPEALVNNTIKHSIRLAEMISEINPSNGLPTFLSGSLEYTYLWPTPGAMACTMLLDA
                     MGYHDSVAAHLEQFRVGQGQFKPPGDLFEKHPGYLSASTGLSAIDWLSDHGAILYSVA
                     EHSLMTGDEEFTRRWLPAIEKACDFIKDARRMQGH"
BASE COUNT         4964 a         7525 c         8379 g         5014 t
ORIGIN      
        1 cgtactcgta gtgggcggcc agggtgtttt cgacgccggt gatgttgccc tggccatctt
       61 tgatcgtctt gacaagctga ccgacgttgc cgtttccgtc atagaagaac cagtacgttc
      121 cgtggaacgt gccttgcgtc tcctcctcca cggccagaag gccggcgatg ccgccggcgt
      181 cgtggatggg gcgggaaagg tccaggcctt gactcgccgc ggcaggtgaa acctgcgggt
      241 tggcgcgcaa gccggcctgc ggggcgggcc gcgtggtggc aagggtgcaa ttgcgatcgg
      301 ttgcgttgtc gccgtttttg agcatgaagc gctccggtcg ctatttgtcg cgggtgatga
      361 aaatgtacag tatggatgac tggttgtcaa ggcatttgaa acctgggctc tatcgggtct
      421 gtgaaggttg ggtggccggc tgtcacagga tctggagctt acgcgacggg ctcttgaacg
      481 actcctggag gtttgttcag gtgtgtgcag accaagaggc agacgcatgc ccgagagggt
      541 cgcgtccatc cgtcccggcc tcaccccaag gctgtgcaag acggacacgg ttgtgaatcg
      601 tccggggaga aggtctgcga gcgcggatct tatctggttc aaccaccgcg ccacgtctgg
      661 accctgccgt ccgcgacgcg caggtcgagg tcatttctgg gactgccttg agaacgtaag
      721 tcgtattatg gcaagcaatt aacgtcgaag accggtctgt tgctgtccac aggggaacag
      781 cggggagagg cattgatata ccgtgggggt gcggaatccc aggtcccgtg ttttgcgggc
      841 ttggacaggg gcgaagtgat gtgggatgta cgggcgttac tcgtcaccga agatggagga
      901 tccgtcaaga tccatctcgg cggtgtcgtc cgtgcccgcc aaggcttcca aggcactgat
      961 ggggtcttct tcgccctttg cggtcgcccc ggcagtggct ggtttagggg tagcgggttg
     1021 cttggcggtc gcaggtcttc gtgtttcgag acgggtgtga acgggtttga tgttcttggg
     1081 ttcgccgttc acctttacgg tgaagacgac ggggccaatg accaagtggt cgcctgcggc
     1141 gagttcctgt tcgctgatcc gcttattgtt gacgtaggtg ccgttggcgc tgccgaggtc
     1201 gcggacagcc accgtgttgc cggttaccat caaaaccgaa tgttttcgcg agatttccgg
     1261 aagggggatt cgcaggtcgc attcctcgct gcgtcccaca agagtcttgc ccggcgagag
     1321 ggggaactct ttgctcgagc cgtcgtcttt gaacattacc agcacaacgt tcatgacgcc
     1381 aacctgcctc aagttgcctg aaactcaggt tttgcgcact gctcgatgcc ccgcgagtct
     1441 cgcgatgccg atgctcgcct ggaggaccag cagcgcgccc aatccacacg cggcggggcg
     1501 tccctgaacg gccgtttgtg aggtgtgcat cagctcactt gttgcgccgg gtccgcccca
     1561 acgctgatta gtctaccgct tggcgtcatg cctggcaagg cttttgggct ttgccggctt
     1621 acaggcgcag cccgccccag gatcggggcg gaatcgcaag aagcgagttt gccgaccttt
     1681 atgtggaagc gatctgagag gcgcaacttg agactcgacc gccctggcgt tctgccaagg
     1741 cggttggagg agggagtccc gaaaggccca ggctgtggcc tatcggaggc ggtcttccgg
     1801 agggcctcgg cccgaaacgg tgccgcccaa aggatgtctg ccgtaggggg acgcgccggc
     1861 ggcggcgtgc ccctgttgct cgggatcgga ggatttccga cggccggagg tagagaggga
     1921 agccgtatgg ttggtgggcg gcggggcgtt caggtttgac tgtccggcag cagcggcatg
     1981 tttttctcgt cggatgcagg gggcaagaac ggtgcggacg tgtttggtcg cgtgagagcc
     2041 gtccggtgct gacaagacct gcttgtctaa ggcggaccgc gttatggtgg gccgcaaccc
     2101 aaacgttctg cgatccagaa acatgcgcgc gagccgcaca gatgtccggc cgtaaggatt
     2161 gaaaggcgca tgcccacgca aagccgtggg catggcaccc gacggacctg ccctacctgc
     2221 tcggtggagg attggcatcg tcatgggcgc tgaatgcgga aaggattccg cgggggatgc
     2281 ggcccggaaa ggattccggg ccgagcggac gagagtgcgg cccggaaagg attccggggc
     2341 gagcggtcct acctccgcat gcccacgcaa agccgtgggc atggcacccg acgatcggga
     2401 gacatgcgcg caaaatgcgc accagtcgcg acggaagaga ctaaggcgga ccgccttctt
     2461 gcgggccgca ggccaagcgc gcgtagaccg gcaatatgcg cgcgaacccc accgatgtcc
     2521 ggccgcaagg attcaaaggc gcatgcccac gcaaagccgt gggcatggca cccgacgatc
     2581 gggagacatg cgcgcaaaat gcgcaccagt tgcgacggaa gagactaagg acgagcatgt
     2641 ccgcgaggtg accatacact cgcacagacg tttgatcaac caactcaagg aagaggttgc
     2701 ccgatacgac gctcgtcagc cggcacgggg gtaggaccac gaccgcgacc gtcctcactg
     2761 ccgaaggtgc gactggattg cctccaggaa gcgttcgccg aactggcggc tcttgatggc
     2821 tcccacccct ttgatatcga ggaactcatc cggagagcgg ggccggcgac gggccatttc
     2881 gcggagcgtc ttgtcgctga agatcatgaa cgcgggaacg ttcaactcat cggcgaggtg
     2941 gcggcgaagg tcgcggagct tgccgagcag attcatggcc gcggcatcga gcggttcgct
     3001 cttgccggac agcggcggca ggcgggttgt tcgtgtgggc gcccgcttgg gacggtctgg
     3061 gtcgtctgtc cttcgctccc ggcgctgacg gccgtcgcgt cgtcccggat cggaaagccg
     3121 ggcgacggcc tgtgacctga gaaccttcca gccctccggc gtcattttga aaacgcggta
     3181 ctcgccgtcg atgcgcagga aaccctggtc ggccagttgg tgcatccaca taatgagcac
     3241 acccttgggc cgatcggtga tcaggccgaa agtgctcagt ttgtcgtgtc cacggtccac
     3301 aatccggtcg gttgggaccc ccatcaagac gtcagccacg tatgcggctc catagcgctc
     3361 gccggtgcgg acgatgcagg agaggatctt ttgggcggtc accgtggagt ccggggctcg
     3421 ctcgagagtt cccgtgcaga cgtcgcaggc atcgcaggat gcgcggtccc acgcctgacc
     3481 gaagtagctg accagccgtt ggtgccggca gcttatgccg gtgcagaagt cgtacatctc
     3541 ggagagcatg cggagtttgt tctcgaggtc ggcggattcg ttcttgcgga tgatcgtccg
     3601 ccaaaggtgg aagtcgccgc cagtgtagaa caagacgcag tcggcgggca ggccgtcgcg
     3661 cccggcacgg ccggtttcct gctggtagtg ctcgatggac ttgggcatgg ccgcgtggac
     3721 gacgtagcgg atgtcggcac gatcgatgcc catgccgaac gccaccgtgg ccacgacgac
     3781 gtccgccttg ccggccgaga aatcatcctg ggcctgtgtt ctgacctcgt cgctcatccc
     3841 cgcgtgatag ccgatcgccc gtacgccggc ccgttgaagc tgcgcggtga gtccgtcaac
     3901 atcatcccga cgaatgcagt acacgattcc gacgtgtcca cgatgctgct tgatgacgct
     3961 caatacgttt gccagggtac tcgtgcggcg gaccacacgg taacggagat tcggccggaa
     4021 gaaatcgccg accaacacca ccggcttccg cagcccaagt tgagcgatga tgtcgtctcg
     4081 aaccgttggc gtggccgtgg cagtgaagcc gtgtaccgcg gcattcggaa accggacctt
     4141 gagctgtccg agccggcggt agtcggcgcg gaaatcgtgg ccccagtggc tgatgcaatg
     4201 ggcctcgtcg atggcaaacg accccgcgcc aacgtggccg agcatgtcca cgaacaaggg
     4261 atcggcgaat cgctcggggg ccacgaacag aagcttgtat tcccgacggg tgattcccgc
     4321 catgacccgc cgtcggtcgg cagcgtccag gctgctgttg aagaaagctg acgagatgcc
     4381 gcgctggatg agccggtcca cctggtcttt catcaaagcg ataaggggcg aaaccaccac
     4441 tgtgagcttg tcactcagca gagcgggggc ttggtaacac agcgacttgc cgccgccggt
     4501 cggcaggaca accagggagt cccgaccctc gacggctgcg accatcgcct catgttgcag
     4561 tgggcggaag gataagtagc cccagtgctt cttgaccact tccagaagag aatccaagac
     4621 cccggccctc gatcttctgc tcctcggtca atgtccatca gacccggtca tgatgttccc
     4681 agcacctggg cccagaatgc ctgggcaaag atgcgcgtga aggtgccgga accgaccttg
     4741 agggcgggga tgcgaacctg ccgttccggg tgtcggaggc cttcctcgat aaacacctcc
     4801 gtttccctga gcatgcgagc gcggattgcg gtccagcttt cgaaccgacc tctgaaaggc
     4861 gagaacatta ccgcgtccct tcctccaccg tctgctcgac cttgttgccg caccagaagc
     4921 cgccaaggac cgcggcgacg ccgaccgtca tgtactcgat gggcaggggt ctgaccagat
     4981 cgtgcggcgg ggttgtcgcc agggccgcat agggtcggta tgaaccggag ccggcccagc
     5041 tcatgtcgga ttccaggtag ccgatgaggt atgccgtcag tccgacgagc acgacggcgg
     5101 gcagatacca gcggacgtta tcgacgccgg tgacggttcg tccggccatg gctccgagaa
     5161 aaagcccggc ggccactgaa gcgatgacct gtccccgggc gatctccgcc acgggagtcc
     5221 gggcaatggt aagccagatc acgattaatg ccacgatcag tgtcgaggcg agcgttgcgg
     5281 ctccaagttg cggcgagact ggtttggtgc gagcggggca ggctgagacc ttgctgtccg
     5341 gccagagcca cttcctggtc agcgtggagg cggcccaagc caaggccatc accgcggccc
     5401 agagcaggca atcttgtccc atcagccgca tcaattctct gcgcgatgcc gtctcgggtg
     5461 aagcgcagta agccaggacc gactgcatgg agccgccgcg gagggccatg gttgccatac
     5521 cgacgccggt ggcgaacaag ccggcttcgg gcagccgttt tccggccacc gccgtggcga
     5581 tggcggaagt cgcggccccc aagaccagca tcatcagcca gatgtggaaa taggatgacc
     5641 gtccgccggc ggtgaaagtc agaccgagga gtgagtccgg gggctgagcc acgttccagc
     5701 cgacgccgta catcatcaga atggccaccc cgcaggcgag ggcgacacgg gtgcggtcaa
     5761 tgagcccgac atgcggcggc gcaatggtta cagcggattc catgccttgc aggctacccg
     5821 atagcgggcg gggaggtcaa cagaatcggg gtcggcggcg aggggccaca ggcgggagtt
     5881 gacatcaggc gaagggtggc ccttgctcca gtggagcaca gaagaggatg ctgcctcgat
     5941 tctcgttccg cttacccttt caagctgaga agcctggcaa gggcgtgcag caggttgttg
     6001 accgccgagt catcgcccaa cgtgacggtc agcttgtgct gacccttctc atcctgagtg
     6061 atgcattcgg acaagcgggc ccggagggtt tcgaccatgt cgtcgaattc cggtgtctga
     6121 ggacgttcgg gcaagagctc ggacatgaag cccagggcgg cggtcagaag ccggccgccg
     6181 gccgagccga cgcgatcggc ccgggccagc cgttccgcct cttccatcac ccgccgcttc
     6241 tcgctttcat ccaccggtgc atacggtcgg gcgccgatca ggacctccaa gcgccgcttc
     6301 aactcgtcta ttccggtcat gaggtcgacc tgactgacat ctgactcggc atccaaagcc
     6361 gccatggcca gatcgtgctt ggccgacagt gtgccgagca ggttctcttc gatggttgcc
     6421 tcggtgacca gcacaaaaac ctggaccggt tgctcctggc ccatgcggtg agcccgggag
     6481 atacgctgct ccaggaccgc cgggttccag ggcaggtcta cgttgatcac cgtgttggcc
     6541 gcctgaaggt tgaggccggt ggcacccgcg ttggtggtga tgaatacctt gattcgcggg
     6601 tcgttctgga aggcgttgac cagcggctgc cgtttcttct gagggatcga cccgtccagc
     6661 cggacgaagg cgatcttgcg tttgttcaac agcggctcga tcaggttcag catcgtcgtc
     6721 cattccgaga agagcaggat cttgcggttc tcctcggcga caagctgttc gagcaggttg
     6781 tccagcgttt ccagcttgct ggaatagccc ggttcttcct tggttgcgag gtaggtgcta
     6841 tccgcggtca tgcggcacaa cgtgagagcc tgcctgagac gcagcaggtc catttcggtg
     6901 atgtaaggct tggtcacgat ggatctgacg atctgggagt aggtctggtg gagtttgagt
     6961 tgctcgtcgg tcggggtaat gcggacgatc tccgtcgttc tcggcggaag ctgtttcatg
     7021 accgagtcgc gtgtgcggcg gagcagaacc ggcttgagcc gggcgcgcaa ctcgtcgagg
     7081 ttcttgtagc ccagaacata gccggttttg tcggtgatgc ggtggcggtt gaagaaacgg
     7141 aaggccgggc ccagccgacg atcatcgatg aactctgcca cggagaacag gtcatccagg
     7201 cggttctcga ggggcgtgcc ggacaggacc aaggcgaacg gcgacttcag gcctttgatg
     7261 acccggctgg tcttggtctc ccagtttttg attcgctgac cctcgtcgag aatgatcagg
     7321 tcccatttga ctttctcgac ggacagaata tcgcgcatca cctgttcgta gttgcagatc
     7381 gtgaagaacc gatcgttgtc gtactgggcg gctctttcag ccgctccgcc gagaacaagc
     7441 tggcaatcac ggttggagaa acggtggatc tcgctgcgcc actgcgattt gagagaggtt
     7501 gggcagacga tcagaacctt tcggatgttg acctcgcggg caagcaactc ggccacgccg
     7561 atgccctgga tggtcttacc caggcccatg tcgtcagcca ggatagccct tccggcaccg
     7621 acggcgaacg cgatgccgtc catctggtag ggcagcagtt cggccttgag cagagtcttg
     7681 cgcaggggat ggtccttggg gtttttccgg atctccgcgg tcttggcggc catgcgctgg
     7741 agaaagagct gctggttgat gtattcctca gcatccggat aaatgaccac cgggtacccg
     7801 aggatggcca gtttctgaat tcgcctgaga aggtcgggaa tgtcttgaat cgcccggccg
     7861 ctaaccggcc cgatgagggc accggctgcg ccattgaggt tgggcggggt cagcaggcgc
     7921 aattcaagtt gctcgccata ctgcaaatac acggccaagt ctttcggtac atacgggcgg
     7981 agccgggtgt tctcgggaaa gcgtttcttg accttttcgg ctacgcgcat gatgtgcttg
     8041 cacgtaccga gcgtgttctt gcgataatca gggcacgaac aataggactg ccctcgttcc
     8101 caaccccgca gggccacacg gtaggttttg cccgacatgc ggttcgtgac caggtagtcg
     8161 gtccagagtc gggcagggtc catggacgtg acggtcatgt tcccggtgcg ggcccgctcg
     8221 cggcgctcct ccaacgcctg ctcgatgagc ttctcctcgc tcaggctctc gaccgggaca
     8281 cgctcgcgag gcggggccgc caggcccaag gacatcttct cctcgatgat cagtgccagc
     8341 gccgccccga tgtgccggca gacggtgttg caactggtgc agttgaagcg caggcggttc
     8401 ttggcttcgg ccatcgcggt gatcgtgacc acggcgtccg gcaggctcag gcggaacagg
     8461 tcctcgccga ggtacacttg ttcgtcgagg cggatctcgt atgctccgcc cagcttgatc
     8521 agttccttgc cgttttcgcc gagaagctga caggccttgg tgtagttcaa acgggagaga
     8581 cgatcacgaa gcgaagggag tttcctggca gtggcggtga gttccattgt tgtgtacctc
     8641 ctcctgggca cggacagccg attgcgatgc tgaacaatgt accgatggca tgctcgtcgt
     8701 ccagatcagt ttcgggaggc ccggatgact gaattcatcc acgggtcttc acggagcaga
     8761 cggacgtctt tgaatccgga ggcttcgagg tcttggcggt actcgctcaa cgagtacgtg
     8821 cccccgcctt cggtgttgac cagcatgttg acggcgaaca ttgccccgcc cggcggggcg
     8881 gtgtggtcgt cgttcatgac cacgtctcgc aggaggagcg ttccgcccgg gatcagggcg
     8941 tcgtgaatcc cggcaaacag ttcgcggttt tgcctccgcg agttctgatg ggcgatggca
     9001 ctgagccaga caaggtcggc gccggcgggg agcttgtcga cgtagaagtc gccgggggcg
     9061 agcctgacgc gctcggcgaa gccggcctcg cggagccgct gctcggccat gggaatcacc
     9121 tcgggaaggt cgaagatcgt ggcggtggct tgggggtcga gtcgcaggaa ggccatggtc
     9181 caggttcccg acgccccgcc gatgtcgagc aggtgcttaa acgggggagg atggatgtcc
     9241 gagacgaccc ggtcggcaac cggttcggaa acgacatgca ttgctccgat gaacgcggcc
     9301 tggtccgctg cttcgccccg gatgctgggt gttcgctcgg cgggacggcc ggtcttgacg
     9361 acgcgggcga gatgcgccca tctgcgcatg caattagcgg tgtgctggac catcggcagg
     9421 accgatcgtg ggccgtcctg cgacagggcg gcggagacct cggccggcgt gcggtacttt
     9481 ccgtgacgtt tgttcaggag cccgatggag gccagcgcat ccagcagcac ggtcaatgcg
     9541 cgggtatcga cctggagtcg gccggcaagc gtgtcggcag ccatctcctg gtcggcgatg
     9601 acggtgaaca ggtcgaggtc ggccgccgcc gcgatcacac aggccggctg gtatgcgcgt
     9661 gcgagatcga ggatttgttc gttcgtccat tgtttcgaca tgacggtgcg ttctccgaaa
     9721 acgtgcggtc gatagcggtc atgggattgc cttgggggag gccggtcggg gttcaccgga
     9781 ttccagtcgg cggtaccagc ttgcggcgag aactaccgaa ccgaccgcga ccatgagcgc
     9841 gcagatgccg gccggcagcc attgatgagc caccaggtaa ctcggcccga cgtacatggc
     9901 tccgagccag aacaggccga cgaccagtgc ggcgatgtcg gggaggaagg gctccttacg
     9961 aaactcgggg tttgtccggc ggatgtattc gacgaccggg ccccagcgtc cggcgggctt
    10021 cgtcgtacgg tagaagtgag ccagcgtgtt catgtcgggc ggcctggtca gcagtgtcac
    10081 gataatgcat gccgccgcgg agaccgacgc gagcaccgga atgacgtagt ataccggtac
    10141 ggaccggccc caccccatgt atccggcaat cggctcgtcg aggaaggttt ggatcagcga
    10201 gaataccatg cccgtccaca tcccggcggc aaacccgtat ccgcttattc gccaccaata
    10261 ccatcgcagg acgttgggaa ccaggacagc cgagccgagg aatccgagaa tccaggtgat
    10321 cattgttgcg atcgaactgc tcacgagcga gatcgcgatg ccgatcaaga tcatcaggat
    10381 gcttgccagg tagctggcgc ggacgtaatg ccgggttgat gcggacggat tgacataacg
    10441 ctggtagatg tcgcggatca gataagaggc cgatccgttg accattgctc cgaacgttgc
    10501 catgaaggct gcgatcagcg cagccagggc aagccctttg agtaccggcg gaagcttatg
    10561 agcgattaca tagggcagca ccttttcggc gtcgaagccg gtctcggtag accctgccac
    10621 cgccacgagc ccgatgatgg tgatggccat ggccatgggg aaccggacgg tgtgacagat
    10681 tccccagagc atgccggcca gcgacgcctc gcgcgtgttg cgagtcgcaa ggaatttctg
    10741 gaagtcgtag agctgctcgg gcccgctcag gcccagcagc agccccttca tcaggaaggc
    10801 gatgaccaca gccccgaaca aagcgtattg gtcggcctgg ttctcgattt gccacggtgg
    10861 tagaagcgag taccactgta cggggttgac ttgaacgatg cggtttgtgc cctctgcgag
    10921 cttggccagg gctgcggcga ccggttgttc gtcgaacgtc gcaaagccga gtacggcgat
    10981 catggcggcg ccggtggtca gtacgatcgt ttgcacgaag tcgacgatgg tcaagccggt
    11041 gaagccgccg acgaccacgt acgcgcccgt caggctgatg atgagcacgg cgcacaggcg
    11101 gtcgatgttg cgtggtgtcc agtcggccag gatgggcacc cattccctga tggggaggaa
    11161 ctgaccgccg aacttgccca tgcccacggc cgtgtagccg atgaaggttg tcagggtcag
    11221 cacggcaaag gtcgtgtagg ccagccgggc ggtgtcaccg cttcgcgtcg gcccgaatcg
    11281 atagaccatc cactcggacc ccgtgatcac gccgctacga cggatccact tgcccatgta
    11341 cgccagatag aacgcggcga tcaggaagcc ccacatccac tggatccaga agccgttgaa
    11401 accataggct acgaacatgg agacaatcca catcgtgccc gtgatgtcga agtagctgga
    11461 agagccgctc atggcgatca tccaccacgg cagttccctt ccgccgagga agtaagattc
    11521 caggcctttg gaggctcggc gcttcaccca gaagccgatg gtcacaacag caccgatgta
    11581 ggcaatgaga atcgccagat cgacagccga catgagtgtt cctcgtttcg cttggacatc
    11641 gagcttgcga aggtcgggca agtgtacctg ttgttgttcg tcttgccagc ctgcggggaa
    11701 tcccggcgag tcttgcgggc cttggcgagg gcaagcgtgg gctcgtaacg gggttagaca
    11761 tcggaccttg gatcgaatcg acccggttgc catcgattga tgggacggcg ggccggtttt
    11821 ctggaaccgc agtgccgtgc ggtgttgtgc agcgagggca cgacagggca tcgattcttg
    11881 cgtaagatta gctttatcaa tggcttatag attgtgtgaa ggttggccgt tcggcggacc
    11941 gacaatagag aatccgagac cggcaagccc gcgaagcggc cgattcaagg ggcgggtgca
    12001 acgtcggcac gccaccttga caagggagat ggccaattcg atgctgataa gcgtctgctt
    12061 tggtgggacc gacggcgagg cagtggaagc cgagcccgac gagggcgccg gttcctggtt
    12121 gccgtggaac cgcggcagcc tcaggctttg gtcgattttc aagtggcagg ggacagggca
    12181 agccttccgg atgatggcct ttcccatgcc acagaaagga atgatgacgg agttgcattt
    12241 gtgaccagaa caattctgac tattcgaggg ctgcataata agaggtcggc gtcgaacgtt
    12301 gcccgcagtt tgaaggccac gaagggcgtg acgggggtgg cgattgacgt gcagtctcgc
    12361 agcgcctgcg tcgaacacga cgagcaggcc aaggttgaac aactgatcag tgcggtgaag
    12421 caggtggggc ttcaggtgga cggttttaag ccgtcggacg ctgaagcgta agattcacct
    12481 catctggttg gcggactgag gttccggacg ggcagaatat gccctcacgt accgtcaagc
    12541 cgtaaggcct ttcctttgca gcacttatgg ttttggggtg tcgatctgtc gccacaggtc
    12601 tatgggcttg tctgttttgg ggggctggta tggccattca attcgccggc cagtgccagc
    12661 ggagtggtag gctgccagga tgattttgag cacctcgcgt ccgtcctcgc ccgtttcgat
    12721 gggtttttcc agtccgcgaa cgcatcggca aaagtgagcc atttcctgcg ggaagccgta
    12781 gttccagatt tcctcgaaca tggtgaaggt gtaccctttg gtggtagcgg ccttctcgac
    12841 ggcataccca taacccggct ggctataggt cagcagggcg ctgccgcgga gcaggtcggc
    12901 tcgcgtgaag ccacctgagc cgtagatctc gcatcgatcg tcgacgcccc cttgcttggc
    12961 ccaactgttc tcagccaagc caacgcggcc gccttcgtac tcgacgatgc agaggctgtg
    13021 gtcttcgccc ctggtcttgt cggcgtgcac gtatgtgccc atttgagcgt agacgctctt
    13081 gacggccggc ttgccgaata cccagcgggc gaattcgatg ctgtggcagc ccatgtccag
    13141 cagcacgccg ccacccgagc gattcacatc ccagaaccac ggcatgtgcg gaccgaaatg
    13201 ctcttcgctc tgcttgacca ggaacgcctt gcccaaggcg ccctcgtcga ccaaggtctt
    13261 ggctcgcaca tacttgggcg caaagcacag ttcctcggcg tacatgagca gaacacccgc
    13321 atcgcggcac gcggcgatca ttccgtcggc ctcatcgagg gtgcgacaga gcggtttctc
    13381 gcagacgacg tgcttgccgg ccttggccgc ggcgaccgcg acctcggcat gcaggtcgtt
    13441 gggaacgcac agattgacca tctcgatatc tttcacagcc agcaggtcat gatagtcgtg
    13501 gaaggctttc ggtatgcccc gctcctcggc gaactttttc gccttgccgg gggagggcga
    13561 cgccacggcg acgacttcgc cttcagggat catcttgaaa gccgccgcat gcagatctgc
    13621 aacaaaaccc gagccgatga taccgacctt gaccttgttc atttgacctc tccacagagc
    13681 atgttgatga gactggcttc aggtaatctc gacctgtcga gctgtttccg cactttgctg
    13741 ttcggccagc gccagggcca cggcgtcgcg gctttctttc ggcgtggtca gcttcgggtc
    13801 ccggttctgc tcgatgcaac tgaggaagta gtcaatctcg gcatagtagc cgtcttctgt
    13861 ggggatctcc ggcgtgtagg ggtccttgcc gttctcataa acggccagcg gtttgccggt
    13921 gttcatgtcc cagtggacgc cgccgttttc gaagacggcg gtgaagccca tgttgaaggg
    13981 gaagtttccc ggcatgtccc accagccctc caattgaacc agggggaccg catcgtagta
    14041 ccactgggca tggatgcggt cgcaactgcc gttgggtttg atgtgacctt gggcgcagac
    14101 ggctttcggc ttgcccagaa gcccgaggac gaaatccacg tcgtgcacgt gcaggtcgag
    14161 gacggccccg cccgagagtt tcggattgag cacccagttg ttcaaggtgt agcccggggt
    14221 ggcggcctgg cggcggaggt gcagagcctt cagttggccg taccggcggt tctcgacaat
    14281 ctgccgaagg aagcggtatt ccggccagaa gcggatcacc tgggcgacca tgaacctggc
    14341 cttcgactta gcggcggcgg ccagcatggt atcgcagtct tcaaccgtca gtgccatggg
    14401 tttttcacac aggacgtgct tgcctgacag cagggcggcc aaggtgattt ccttgtgcag
    14461 cggcgtcgga acgcagatgt ccacggcgtc gacgttcggg tcggcgatca actgcttcca
    14521 gtcggaatat cgctgcagat ggctcacgtc ccgcttactg cccttggagt cggcgatatt
    14581 gccgccgacc ttcgaccagt cgccttcgcg gcgatcgggc tccttgtcgc agatgatgag
    14641 agtcacccgt tcgggcatct tctcgtactg gttgaagtgg ttgcgtccga tgaaaccggc
    14701 gccgatcagg cctacgcgaa tcatgagatc tctccataac gcaagcgtgc gatgctttgg
    14761 ggcacgcgac gagccgggga caaaacccga atgtcggaat cgagttgtca gtcatcagtt
    14821 ttcagtctcg ggggctttga agaaaccggt tactgacaac tgagcactga ccacttctat
    14881 tggctttgtt cgtcattctg gtttcgtcat tcgtcatttc caggcctccc cagcgggccc
    14941 gggatcgcac cgaaatggta cccgggtctg cccgctggcg aaaggctttc tcgtgcgtta
    15001 cattctggcg acatgagaaa cgcaattcca aacaccatca tcgtcacagg actattcagc
    15061 ataaccgcct tcgccgccga catcccgtgg cccgagcacc cgcgaccgga ctttcagcgg
    15121 agcccgtggg tcaaccttaa cggccagtgg gacttcgact ttgatccgca ggatgtgggt
    15181 gaaaaggagc agtggtttgt tccagtccag catgcttttt ccaggaaaat cactgtgccg
    15241 tttccgtggg agagtaagct gagcgggatc ggcgacaagg actacaaggg cgtagcctgg
    15301 tactcgcgcg agatcgcgct gcccgatggc gaaggctgga agggcaagga tgcctggttg
    15361 atcgtcgggg cgtgcgactg ggaagcgagg gtgtgggtca acggccagcc ggccgccgag
    15421 cacgtcggcg gctatctgcc gttcgaggtg aacctttcgc agttcgccaa gcccggcgag
    15481 aaggtggcga tcgtcatccg cgccaaagat gtcaccgacg accagcaacc gaccggcaag
    15541 cagatcggct ggtacacccg aaccagcggg atatggcaga cggtctacgt cgagccgcgg
    15601 ccctcaagat acatcaactg gatcaaggcc taccccgata tcaagaccgg cgaggtcaga
    15661 tttgcagtct catgcatcgg aagcggcatc aacagtctgc tcgtcacgag ccctgacaat
    15721 tcattcgaca cggtagcagt cagcctttcg gcaagcggcg gcgacacctc cggagaaccg
    15781 cgatcggcga agatcagggt caaagagccg cggctgtgga gccctgacag tccacacatc
    15841 tacccggttg ccgttgagat ctcttccgag ctggcgtcca ggagtaagga tcgggtcgag
    15901 agctacttcg ctttgcgcga catctccgtc ggccctgccc ccggtcgcga ctatcagtac
    15961 gtttgcctga acggcaagcc catctacctc cgcggggcgc tgcaccagtc gttccatccg
    16021 gatgggatct atcaatatcc gaacgacgcg gtgatgcgca gcgactacga gctctgtaag
    16081 aagatcggca tcaacttcct gcgcattcac atcaaaaccc cggtgccgcg cgagctgtac
    16141 tgggccgaca agctcggcgt gatgatcatg caggacatgc cgaacttctg gaacaacacg
    16201 gggcaggccc gcaagtggta cgaagagatg ctgcacggag cggtggcccg cgatttcaat
    16261 cacccggcca tcttcgcgtg ggtggatttc aacgagacct ggggcctgga caatcccggt
    16321 ccatatagcg aggaacgcca gcagtgggtc gagaacatat acaagctgac caagttgctg
    16381 gaccccacgc ggttggtcga ggacaactcg ccctgcaagt acgaccacgt ggtgaccgac
    16441 atcaactcgt ggcacttcta catcaatgac tacgagcagg cgaagaagca tattcaggag
    16501 gtcgtggaca agacttatcc gggctcgacg ttcaactaca tcggtgggcg caggcaggcg
    16561 gacgctccgc tgatgaacag cgagtacggc gggatcagcg ccggtcacgg cgaccaggac
    16621 atttcctggt gcttcaaata cctgaccaac gagctgcgca agcacgacaa gatctgcggc
    16681 tacatctaca ccgagctgtc ggacatcgag tgggagcaca acggctacgt caattacgac
    16741 cggtcgccca aggagtacgg ctacgatttc tggttccccg gattctcgct ggcggatatc
    16801 aacagcccgg actttgtggt gattgacgcc ccgccggtga tcgaactgaa ggatggtcag
    16861 ccccgcgaga tcccgatcaa gatcagccac tggtccgaca aacaggccaa ggcactgacc
    16921 cttcgccggc gagtggattg gatcgatcgg gacggcgtga agtgggaggg tcaggagcgg
    16981 aagacacagc cggccatctg gaagccgtac gaggtcgtcg atcaaccggc gatcaacgtc
    17041 tctgcggaat ggggggtggg gccggtcgtt ggggccctgc tggtcgaact ggtggacggc
    17101 gacgacattt tggcccgcaa ctacgtcaat ttgctggtgc ggcggagcgc tccggcggcg
    17161 gaggttgtgg gcaaggcatg cttcctgcgt ttccggccag gtgacttttc agcctggcag
    17221 tgggaggaag acccgaccac gatgccggtg gcagtgagcg ctgacaaggc gtgcattcgg
    17281 tggagcggtt acatcgagta ccagatcgat acagggatta aaagcccgga cggcctggtt
    17341 ggtctcgatc tgacggccga ggtctcggcc aaggccgggg acgagaagct ggactggccg
    17401 gagcgcaaga aagacattga ctacccgcag accgatgtga aaaagtggcc gagcgatctg
    17461 cggatcagca tcaacggcgt ggagatccat cgtgaaacgt tacctgacga tccggccgac
    17521 gcacgtggag ttttgtcgca tcatcggcag taccaccccg gctcatacgg gttcctggta
    17581 acggcgaatg ttcaggacga atcaatagtc aagcggatct tcgacgccgc caaggacggt
    17641 gtcctgacca ttcgcttcga ggttccggcc gacgcaaaga acaagggcgg cctggcgatt
    17701 ttcggcgacg cgcttgggtg ttatccggtc atgccgacgc ttgtcgtcta ttccagagac
    17761 gagatgccac agccgccagc ttcgttcacc gctcgttcca cggcggcagt caaccggttc
    17821 gccgatcggg cgaaatcgct gctgccgacg gccgagggcg gcggtcatac ctggcgatgg
    17881 accaccgaca ggccggacga caggtggaac cagccggatt tcgatgaccg gcaatggaag
    17941 accggcagga gcggcttcgg caaggagggt acgcccaaca cgatcatcag gacgccttgg
    18001 acaagccagg acatctggct gcggaccgaa gtgacgatcg aggacccggc caccatcgtt
    18061 gccggccaat ggcgacttta ccacgatgag aactgcgaag tgttcgtgaa cggcaagcag
    18121 gtcatcaggc aaaggggttt tgtcacgcag tacaaggacg taccgcttgg ccgcgaggct
    18181 ctgtccgccc tgcgtcccgg caggaacatc atcgcgattc actgccggca gaccgaaggc
    18241 gggcagtaca tcgacgccgg gctgacggtg ctggtgaaat agtcaaacga cgcggtccgc
    18301 gggccttctt ggcatcatcg gcgtggttga atcaggcggg gtcgtccggc attgaagatg
    18361 tcggcgcgag cgtctatgtt tgttgtgccc ccgagtgaca cggtaaccgg cgcacgaata
    18421 gacgtcatcg tcaggaggat cacggtccat gattcgtcca agcctgcttt tgctgcccgt
    18481 actcttctgg gggtgtggct ccggcacccg gagttgcctc gacccgagcc gccgcagcgc
    18541 ggaagatccg gaagcgattc gtatcaagcc gcatcatttc gtcgacatca ttacgtcgta
    18601 tgggcgtgga gaagaattca agccgcatcc gtacggccac gctgttcaca cggtcgccga
    18661 gagaatcctg agtaaccgtg acgtcatgct ggtcatggag ctgggagcgg atgacatttg
    18721 cgggccgtgc aagcacaacg tgggcggcgt gtgcgacgac gtcatcgata cgtcctatcg
    18781 accgcaggcc ccgtcccgca agcaggaatg gaacctgttg atcgacgagc ggtggtgcaa
    18841 acgactggag ctcttgcagg gcgatcgcct gaccgcccgg cagttctgcg agcggctccg
    18901 cgatcgcgcc ggtgacatca cggacatcta ccgcgagatc ccggcggaga tgaccgccga
    18961 gcgagtcaag gcgatgcgcg agggcgtgcg gcggttcttg cagggcccct gatgcactcg
    19021 gccgagccgt ctgctatgcc agccagggac gtttcctttt ctgcgcgtcc ggccacaggc
    19081 cgacgaggat attgacgtcc tgaaccacct ggaaatcgaa catgccgacg caggcgaagt
    19141 cggcctcgtt ctgaaacgcg taccggaaac cagcctcggg ctcgatcgcc ccggccgcca
    19201 ggatcttgta ggggatccag ggtttgttca ccgtttgcat gaagtcgatg gtccgctcgg
    19261 gttcgaggca ccagtagttg tcgtgcgggt tgtgggatac gtcgtgtcct tgccgcaagc
    19321 atgggtaatc ttggacggcg tgcccggggc gggacggagt gacgagggat cagccaggga
    19381 tgtcggcgtc ggtctcctgg attgtcttgc ggtagttcca gggcatccag ttttcgggtt
    19441 gggcggcgag gtcttcggcg tggcgttgca gttcagtcag gtagtcgaag ggatcgacat
    19501 cgttgagccg gcaagtatgg atgaggcccg cgaagatgtc gtagaggggg ccccgtgccc
    19561 caatgttctg gaaatgcccc tgtgctgctc cagactgttt agcgacacta acacattccc
    19621 cttgttaacc agcggtttgc cccaaagacc agacagccat tttgagcaac tgaccgtcag
    19681 gcatcctcga tggcacgtta gagggcgata tctgacggtt gattcagccg gaatctgatg
    19741 gttcaatcga aggaggatcc tgtctgagag caacaaaaaa cctccgtaag tcgctcacct
    19801 acggaggttt acatcatcga ggccgacggg gctcgaaccc gcaaccttcg gatcgacagt
    19861 ccgatgctct agccaattga gctacggccc cttcgggttt ccccgcggga ttatcgtgag
    19921 tatatcctgc ggggggttca agtcaaggtt ggcgggttct ggtcggtcct gtgtccaagt
    19981 agtttgcttt tgccattgtc cttggccggt tcgccgttta tgcggtcctg tttggaacgg
    20041 caatcacgcg ctccctgcga acagccgtcc tcggggtcat ccctcgggcg gcctgcgtgc
    20101 cggtcagagc gttcgttaac cgaacgcttc aacggaagat cagtctcgcg atcaccacgt
    20161 aggcgttgct gaacggctcc tggcggaaga aggcgacggg tgctttggga tcgccgatga
    20221 agggtcggag catccagccc atctgcatcc ccacgaaggc gtagaggccg acccagacca
    20281 tgagcatcag ccggtggcgg cgattccggc ggaccagtgg tcggtagtaa cgcagcatga
    20341 tgatttgacc cgccgcggtc gcgaccgtga acatggcggc gttgaacagc agcgctgccc
    20401 ggtagctgcc gcacgagaag taccagaacc gcgtcaatgg ggcgagcgag gccagggcga
    20461 tgctcagccc ggcctggccg gcgagaacgg cctgcacggc ctcgcggaag tcgtcccgca
    20521 agccgagcac ggtgttgagc acgaagaatc cgggcaggca cagtgcggtc gtcgcgaaca
    20581 gcagcaatgg taccttggcg ccgctgtaag ctattatgag cagccgttca gccgagccga
    20641 gatcgaaact gcccatgaac gcgccgtaga tcggcgagaa agcgactacc atcagcggga
    20701 gccaccaccc cggccgtgag gcagcctgct ccggcaggaa cggtccgctg cctcgcagga
    20761 agagatcggc atgtgccagc gcggttcgca tcgttacaga cgcccggact caattcaacc
    20821 ccagcaacgc ttgaatctga tggaacaccg cttggaagaa attgcctgac ctcggcctga
    20881 accaactgaa gggcatgttg gggttgccga tgaagggtcg cagcacccag cccatctggg
    20941 ctcccaccag gcagtagatc agcagccaga tccggaagat cgtcttagcc tgtcctatcg
    21001 cgtagtcggg catcggcgag ggttgtgggt ccagcgggcc gagttctctc ggaggggcgg
    21061 ttgagaactc tgccagtgac gaccgaccca tatcggtggg ggcgtcaggc ggaggcggtg
    21121 atgttggcgc gggtggcggg acgaccggat atgccagccg ttggagcgta ttcagcagga
    21181 aagccaaggc cacgcagccg gccaccgcca gaaatgcgac gttgagcaga accatgaacg
    21241 ggtagctggt cgtactgacg gtgaaaaacc cgagaatagg ccccaatgaa gcagccacgg
    21301 ccacgttgac cgcaatcgct ccgacgagca ggcgcagagt ggccgtgaag ctcaagcggc
    21361 agcccaccat ggcattgaac acgtacaacg atggaaaagt gaccatcaac gtcagcagaa
    21421 acaaggccgg aagcttgacc atgcttgcga ggagctgcca gcacctttgc ggctcggggc
    21481 tggttccgcc ccagtagata gacagggcgt accagcccat gaagaaaccg tagctcgccc
    21541 ccagaccaac ggccagcggc acgaaacgcc tgagggacag ctccaccttt ccttccgcca
    21601 gacgtcctgg tgatgtaata cgaccgcgca acagctcgtc gagctgcttg aacgccgaca
    21661 acatatttcg tcctccgaaa aggataagag ggaccggccc gagccgggta tcactaccgg
    21721 accgcgcctg cctcgccaat gcgatgccgg cctcgcattc cgcctgccgg gcatgtcgta
    21781 tttacgttcc cgccgaccac cggttcgcgc cgtttttgtg gtttgtgcca tcctcccagg
    21841 aaacgtggct caagagcccc gttgagaata gacttgcggc gtgtcggcgg tcgtggcggc
    21901 cgccgtcgca ggctccgcaa gagtatcgtt ttccatcgat tgccgcaaca ggtgtgagtc
    21961 gatgcctccg gataccaccg cccagcagcg acaagccctg accgagagac tgctcgcggc
    22021 attcgacggt tccatcccgc gcgtctggaa atctccgttc tatctgttgg gcttgttgat
    22081 cgtcgcgacg gttattctgc tcctgcccct ggtttatctc ggcttcatcg cgttgacggg
    22141 ctacgggatc tactaccaca tcatccacaa tatcgatgtt ctgagcctgc cttctgctgt
    22201 gcacagacga accgtgttcg tgatcatgct gctgaggctg gcagcgtact ttggtccgat
    22261 cctgatcggg atgattctcc tgctgttcat ggtcaagccg ttgtttgccc ggccggcccg
    22321 cagcgagcgg cggctctcat tcgtacgcga gaacgaacct atcttatttg cctttgtaga
    22381 gaagctgtgt gaggttgtcc gggcgccggt tccgcgacgt atcgacgtcg attgcgacat
    22441 caatgccgcc gctttcttcc ggcgaggttt tctcagcttt ctcggacgcg atctggtgtt
    22501 gcggatcggt gccccgctgg tggccggcct gtcgcttcgc gagttcgcgg gcgttctggc
    22561 ccatgaatta ggccacttca gtcagggcac gggcatgcgt ttgacgtaca tcatcggttg
    22621 cgtcaacggg tggttcgccc gcgtcgtcta ccagcgagac acctgggacg atcggttgat
    22681 cgctctctca caggaacagg gatggttcag cctcttcttc ctcgtcgcaa ggctgttcgt
    22741 ctggctgacc agacgaatcc tgtggctgct gatgatcgtc gcttatgcta tgagctgcag
    22801 tctccttcga cagatggagt acgacgccga tcggtactgg gcacgcatct gtggaagcga
    22861 gtgctccgag tcgagcctca agaggctgca cgctctggcg aaggccagcc agacggcgtt
    22921 tgcgttcatc gctcattgtt ggcaggaaca tcgcttgccg gaagacttgc ccgcgctgat
    22981 tgtggccgag gccgaacgtt tgtcaccgga ggcccggcag gagatcgaga aaggcatttc
    23041 cgaatccaga acgtacatgc tcgcgacaca tccctccgac cgcatacgca tccaacgaac
    23101 acgcagtgaa aacgagcctg gaatcttcca tctggcggag cccgcgtcgg tgctgtttgt
    23161 ggatttcgat gccctctgtc gtgaggtgac ctttacgttc tatcagggcc tcatcggccc
    23221 ggaggtccgc cgcgaacacc ttcttccaag aagcgacgtg gatcagaagc gcaggctcct
    23281 ggacgagacg ctggaggctg cccggcggtt ctggaccacg ctggattttg tgacacggcc
    23341 gttccgcatc gaccgattct ctccagtctt cgacctgcct ccaagggagt gcctggagcg
    23401 gctcaaacgc gctcgctgcg ctctcgaacg ttcgcagcat acgatccgca aggaatacgg
    23461 tcggttacgt gaacttgacc agaccatcag caacgcccgt caggcagagt gcctgctcaa
    23521 ggccgggttc cgcgtcgatc cggagatgat tggactcaag aaagctgacc ccttgcaggc
    23581 acatgaagtt ggtcgccggg ctgagcgcga tcggcaggga gtcgaggaac gtctgctgaa
    23641 gatcgaggcc gtctttgcgg ttcgactgga agccgctttg gccttgctca agtgtgaccg
    23701 ggtggccgct cgattagaga acgcgggcgc tttgcgggcc aagagcgagc aactgctcga
    23761 cgtgacggcc ggcctgtatc gagccgagta tcttctgacg agcctgcggc tcaaccagaa
    23821 ctcgcttcgg gcaatggaag acgcgatcag ccgcgggtac gaggaagatg cgctgatcgc
    23881 ccagaccttg agattgctgg agactcagaa ccgggcgttg gcggagttgc gagaagcgac
    23941 caaagaggtt ccatatccgt atgagcacgc aggtggtccg gtctctgtcg cgcagtatgc
    24001 catcggtatc gtcccgtccg tcaacgacct gaccggtacg ggcgcggccg tcagtgcctg
    24061 cctggacaat ctttttgcac tttacgtcag ggttatggga gagctggccc gcatcggcga
    24121 acaggtcgag gcagtggtcg gcctccagcc acttgccgtt accaaagacg ccccagcgac
    24181 cgaccggcct ggcgttcagt aatcctccag agtcggcccg ccgaacagcc ctggaagctc
    24241 gtagcggcac cgcgagctat tcgaagacaa ggctatgcaa tacttgagaa catggtatgg
    24301 cgaatcgccg tgcctggggc gccaactgga catcttcagg gcgcggcgta taagagttgg
    24361 gcattggcat gcgtgctcgc gcggtacccg gtcacgttcg agaagtccca gacgaggaga
    24421 atgaccatgt ttaccatcca cccggcaggc gcgtcggtcg gccgattgag ttcatgtctt
    24481 gtcgttctgc cggtccttac cattaccatc ggctgcacca ccgggccgaa cggcctcgat
    24541 gtcgatcgag cctggcggga agcggaggtt gccagacggg gggcctgtga gcctaacgcc
    24601 gacattgcct gcgcgtggtg gccggatctg cgggacatct ggactccggt cggctggaag
    24661 gaccacgcct tccgtttcaa cgtgctgttc aatggcacga tcctcgctca gccggacatg
    24721 aaccctcgaa cacagaaatg gaagaaccag ggcgtgcaac tggcctttga gccctcctac
    24781 gacggcaagt ggcgcaacta tggctacatt caccgggacg acaccatggt ccgccagtgc
    24841 tggaacgagg cacatgcagc gccgatcgta cggagtgaat ggactcagca cggcctgtcg
    24901 ctgcgccagg aggtgttcgg ccacgtgccc ggagggaagc ggctgaccag cggcactgag
    24961 ccgatcttcg cgtgggtacg attgtcgatc gcgagcatct gcccgggcgt accggcggag
    25021 aagaaccaca ccttccttat caagcttaac gcaccgcata tctcggcggg catgacctac
    25081 atgaacaaca tccagtttca cctcgaccgg tcggcctacc cgcggaggct caccgccgag
    25141 ccggcgggtg acggatggcg tctgttggaa gaggggggca aggtccggct tggtgttgcg
    25201 agaggccggc cggtcgagat caatttcacg cccggcaagc ctacggagaa ggactcgctg
    25261 attgcgctga ccatggaggt caagcccggc aatcacgttg acgtactcgt tccgatgttg
    25321 ccgacggagc gtgaggtctt cgacgccgag ttgatgcttg gccttgaggg ggccttggcg
    25381 gaggcggatg ctttctgggc gatcgtccca gccactgccg ccgtggtgga cacgccggaa
    25441 gcgctggtca acaacaccat caagcacagc atccggctgg cagaaatgat ttcggagatc
    25501 aatccgtcca acgggctgcc aaccttcctg agcggttctc tcgaatacac gtatctgtgg
    25561 ccgacgcccg gagcgatggc gtgcacgatg ctgctggatg cgatgggtta tcacgacagt
    25621 gttgcagcgc acctggagca gtttcgagtg ggccagggcc aattcaaacc gcccggagat
    25681 ctttttgaga agcacccggg ctatctgagc gcctcgacgg gcctgtcggc gatcgactgg
    25741 ctgagcgacc acggggccat tctgtacagt gttgccgagc attcgctgat gaccggcgat
    25801 gaggagttca cccgccgctg gctgccggcc atcgagaagg cgtgcgactt catcaaggat
    25861 gcccggcgca tgcaggggca ca
//
LOCUS       DAOTPW010000052        25675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_1998_length_25675_cov_5.651835, whole genome shotgun sequence.
ACCESSION   DAOTPW010000052 DAOTPW010000000
VERSION     DAOTPW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1998_length_25675_cov_5.651835"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="P5316_06690"
     CDS             complement(<1..1486)
                     /locus_tag="P5316_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84680.1"
                     /translation="MRRMLPVLMALLAIPAVAQAGEVIEVLSEDFESGLDAWTTGRTP
                     YVDDYPDRMNVFDGSGAVISSDPRDQNMFGPMSDDWGVWGNSQCVGQSSKTWDGTSDH
                     WIQKQWPGALAPGTYSVTLEYDFYVYADPATLCGGRWEVGNRVYLLTDEDFDYPCFNF
                     DNHENLEAPGFKVDYWPGYENYATCQDVGGELRCVGPASGSGEQPCTVTTDCQNAGAT
                     PTAWIYNGKWRRVTWEATVTTTTGNLELRLLKHEKCSGLETVAWDDVDLVIRDASEQV
                     VFEFHDNFDGPDPLADWTQVISCREPVSNNDEPKLFAANDPLLYKNIDNPGSQSAGYS
                     SAEYFKDKTGHAWMKRLFPNALPPGTSGTMRLEFDWYVYRRLQNDGVDNDSPTVFPAP
                     DGAGIYGPNTGYPGSVSVGYASNLIRDGIRYAWIQKQFPSALPAGTYDVTWEMDLYVY
                     SIKTGDYAHGNRLYLLTDTAYNNPTWNPDGSMPANSGGSFRVSYW"
     gene            complement(1700..2632)
                     /locus_tag="P5316_06695"
     CDS             complement(1700..2632)
                     /locus_tag="P5316_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR84681.1"
                     /translation="MQRTCKKTPPVCGFTLIEVLVVVAIIALLVAILLPSLSRARAQA
                     RSVACLNNLRQIGMAVQQYAVQNADIVPRACNDFDPTNWAVVAARSIGAIKRIPAGFT
                     VNQLRVAEIELLHCPERMLSMDYPFLDYVVSSMDPDGPRAGGETGTPNPTGEWYQVYH
                     FQASTAPRCRTNTYRSPGEVVYLLDAEREDRNTASVGAISLAQAHQNYRTNQATEWWM
                     GVMDVWRGLHVPQGKNNINTSDAPGPRRAARNMHLGRFTNAVFMDGHAAGVPLAQRSL
                     PTGQPDHIAQYAYWLRLLGVKNAATIAALDPDLQ"
     gene            complement(3032..4141)
                     /locus_tag="P5316_06700"
     CDS             complement(3032..4141)
                     /locus_tag="P5316_06700"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRR84682.1"
                     /translation="MTQDKILAPYAEAAYFINLANLKSHTSAGVTLCGKNYYGFLRLP
                     TEGGYYNLHDSLPEYVTGMGRYRCMVDLMGHAHTGGKALLYLIDGLYAGVHPIEVAPR
                     KWYTDPFNGDWTSSLFISQDPIAIDSVCFDFLWAEWTDYPHMSGADDYLHEGALADNP
                     LSGTFYDPDHPTPTVRMASLGVHEHWNNPRDRQYSRNLGTGNGIELVKGGCPVAVIAA
                     DPTSGKAPLTVNFDGSASYDTDPAGFIVEYHWDFETDGTVDATGPSATHTYTSVGDHL
                     ASLTVIDNDGERETSSLRISVEYQPGDFDADYDVDQADFGTEQGCLSGNGTNVSGICV
                     RADLDHDGDVDAVDIEIFRGCLSGADVPAERDCAD"
     gene            complement(4138..5124)
                     /locus_tag="P5316_06705"
     CDS             complement(4138..5124)
                     /locus_tag="P5316_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84683.1"
                     /translation="MTDPRACPPASPCPPNAHRPAGPRHLRWQWLFPVAGLLSLIWFL
                     ARVVPKPLRATYPCQRIAAPMAGGFVLWIVGAITSALAYRKARALLGRAQLKLALACF
                     AVAAVAGVIAVYYTPDRFAEAANPEPNAPIGEAKGIFPGRVVWVHDPDATDWVGPGYG
                     HWWEPAHTNQTVVDAMMSRAIRSLTGQTSDTAAWDALIRHFNVTHGRGNVGYTPGEKV
                     AIKVNFVGFIDMGGWGGVDPNTYNLVSKQDYMNTSPQMMLALLRQLVNVVGVNQADIS
                     IGDPVCLFANEYYNLLHDEFPNVRYLDHLGKFGRTKPQASTVISISALARAG"
     gene            complement(5384..6772)
                     /locus_tag="P5316_06710"
     CDS             complement(5384..6772)
                     /locus_tag="P5316_06710"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRR84684.1"
                     /translation="MLAVLVVLLGTVGRTPASGGLIAPGATLVPVRSDSRFYEGPSWD
                     TFTEKLYFSALSTNELFRLTPPDTSALWMSDTRGINGTYLANDGRLLCAQGSARRILS
                     LRIGPNGPEDPIILAENPVWNAPNDLCQTPAGDIYFTTPDFGAQTTSKVYRLTSTGAT
                     TAVITDMPLCNGIIGSLDGSILYVSDSSRKWWRSYPVNPDGSVGAGSLFFNPSTPNQN
                     DPDGMTIDEFGNLYFAGRGGFWIVSPDGQQLDMVAVPEFVTNLTFGGTDGRTLYITCD
                     GKLYSLAMEVRGGRWHNVPEDNQPPAVNAGPDLRTTSRFPTAWLDGSVTDDGLPDPPG
                     ALTVAWSKVSGPGDVDWSAPTAARTLATFSTVGTYVLQLMAYDGIRSAIDQVTVRVLR
                     VGDLDDDTSVDQDDVALFRECVTGSGNGPPITACELADIDADQDVDQTDFGLLQRCIS
                     GPGAYSDPHCAG"
     gene            complement(7073..9568)
                     /locus_tag="P5316_06715"
     CDS             complement(7073..9568)
                     /locus_tag="P5316_06715"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HRR84685.1"
                     /translation="MRSIPISLAGLLLALPAVSMAGESLVNGSFEDGTNNGWIIWAQT
                     WGTGQTWYHDPPAETDVCGHGLTAPDGRYFGGISYLKGTTPDFQGHVLYRQPLEVKNW
                     HPEADRVNYDLELWAQVAHSAQYTGYETRQEHILWWALDGEMPHDTAFVRIGNTVADG
                     DTIEIDTGGVNRVYEFSTDGVLRDPANVLVDVSAAGGGAGVANAKTKLAMAINDDASA
                     PCTASVGSKVVVLLWKETYANGATNTNLNDAGNVIEVTNFSTLPGTPQYETNQDKQYD
                     ENVPMKRVVLTQLKTTFFVTRNDANEVNQCFTNWRKIKLSGSVPGNPKVVVLEIRLKT
                     DSRLTNLHNLFDGVVFSATASTGSLMGKFEGDIPNSDFEDPPYNTQHHGDKTGIRYDP
                     PSGWGDRPPPPPEGYRNYLRDWGRDGATRGPVEGYTNPGTEPPPLPHGTDPLGAFTPS
                     GTHFWGRANPGGENGTGVQGYWGHVVPIRNWSPCATGLRFKLRYLTLMKDNAGSQIME
                     LCWGDTAWAKQAGNPNPATRSILEQPTDALNFDLLQSYQVPWMKIIDFSTALIGDVCA
                     TKGFVEIERTGEFSPTDLNGAASCPQYILLRNSTLSSTATLPFSHFYDMVDFYAEAVV
                     PTDVRIVTQSPLPAAVCDSLYNLQLIGCYVEPLRFSVASGNLPPGLKLSPNGLISGQP
                     NTAGTFAFTVQLEDGASATATKTFEITVNGNCCGPVRGDLDRDADVDQRDFALMQLCY
                     TDSDGGIPMPPDDPDCTCADLDADGVDIDQADLLVFEQCASGPGVPANPQCSMEACCL
                     TEGKCVEILPGTCRNLDGVPQGVGTTCETTSCP"
     gene            10446..12101
                     /locus_tag="P5316_06720"
     CDS             10446..12101
                     /locus_tag="P5316_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84686.1"
                     /translation="MRKSILVTVFPVVLGLVSHWAAGQCEFVATSVTPNIAGRHLNPP
                     YLYVVFKASATDPSIHVNDGDLMTFDTGAQFGGPYVFEFDTDNSVAPGNIAVPLTATD
                     NFTSSRALVEVVKAHPSLPFSALVAPVGPDDQYVSFVFKKNEGYGAGVSRTLAVPGAI
                     TGSATSFAYQTSGNVWFQIAGSNLHPGSPHIVAKFVSQADPTKVITTTSTIRTAEVLT
                     VRASLGLGATPFPAGVYDLVLSRATTPACPDFVLPGALTIVDNLLSDPTFQTNILGNA
                     GTLMWNVEYNTAWLPLVWHHNNVNITNSFADGVFYEAVKRGDKFHPMQQEKPTRKEAP
                     RQAPFPTGPLESTAGDNEWATLRWDPANGIGIGRHQFWQYIDINLSETSFVTLSGVWL
                     GGTTSPSTLTYGIEIRDGDENGTILAQTPPQQTIDSFYWTPFSYVMTLPAGQRRVACV
                     IYGDNSDDVDAGLHIDDLLLRVGAEFWPDVDGDGDIDQADFSSFQACYTGAGAGVVAA
                     GCERFDREADMDVDAADFEAFERCATGPGIRLTPVAVAAGCEP"
     gene            12417..14321
                     /locus_tag="P5316_06725"
     CDS             12417..14321
                     /locus_tag="P5316_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84687.1"
                     /translation="MRSRLLPLITLILANHAGAETLTLPLEQRPAWLAREGIVMAGSW
                     EPLLFRVRRDGSDGYTPTPQQVADYRREHSPEMVARLKSLGVNFVMMHSYKAFGREAE
                     RESMQDAVRFAKLCREAGLRVGVYNNSGTLGWELLFKELPEAKDWAVLDAAGKPVTYG
                     RATYRYYWDRNHPAAQAFHKEIIRFSVEQIQADLLHFDNYIIGPGHNANSVSRFRSYL
                     KSRFKPQQLEEMGITDPDRAMPPTGPDANGLLGRAWLDFSCGSMAESYHDLSRYARTL
                     RSDVLVECNPGGPGNRISPPIDHGRLLVGGEAFWDEGREPGYRDGQLNTRIRTYKLAR
                     RMNNIAFAYTTSPLEAAEAMAFNLDCFGCICWFEYGRLAAKPGVETPVSLELAPYIRF
                     FRDRRDLLRNAEVVADVAILRSFPSQVYAEATQAQLTAQAEQFLIENRVPFQIIYDDH
                     LSELSRYRALVLAGCVAMSDAQIEQVRSFAAGGGRVLLVGDAATHDEWLMPRRAPGLQ
                     DLPEAQLVRIQKIAEMGDAVSTLLQASPSLAVEGPHGLCAELTERPGRRLVHLVNYRT
                     DTPARNVTVTVQLPEGKQVKNVRLARPEHDKDADLEIRQQGRVITCVVPVIDVYGIVV
                     VTRDTPGAPR"
     gene            14459..14770
                     /locus_tag="P5316_06730"
     CDS             14459..14770
                     /locus_tag="P5316_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HRR84688.1"
                     /translation="MRRVISIRRLREFWKEHPDAETPLREWYKIARKAQWHSLQDVRK
                     TYPKADAVTAGSGATMTVFNIGGNKFRLITSIWYPGQQIYIKMVLTHAEHSKDKWKKQ
                     L"
     gene            14791..15216
                     /locus_tag="P5316_06735"
     CDS             14791..15216
                     /locus_tag="P5316_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRR84689.1"
                     /translation="MPRTTISRSPRKLPGRFEDLVALLAPRAIRDEIDYESMIEMLDR
                     LTSLPRLTKGQEQYFETLSVLVEAYENDHHAIEVADLPPIQVLKHLLDSSDMNASQLG
                     ELLGNRSLGSKILRGQRELSKNHIRLLAERFNVDPGLFI"
     gene            15594..16982
                     /locus_tag="P5316_06740"
     CDS             15594..16982
                     /locus_tag="P5316_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR84690.1"
                     /translation="MTTADRASTIWLTALSVVMPVAATQAAQAEPAGKAVKRPNIVLI
                     VSDDQGYNDIGIQGCEDIPTPNMDAFARGGVRFTNGYVSCPVCAPTRAGLMTGRYQQR
                     FGFEFNPGPEGQASDRFGLPLSEKTIAERLKPAGYVTGMVGKWHLGFKDDMQPPQRGF
                     DEFFGFLAGAHQYLSRDGGAAAPILRGQEQVDEKEYLTDAFKREALAFIDRHKNETFF
                     LYVPFNAVHAPLQATDKYLKRLDGISDETRRIHAAMVSALDDAVGEIMTKLRDSGVEE
                     NTLVFYLSDNGGPTRQTTSSNKPLRGFKGDVFEGGIRVPFMVSWKGRLPAGKVYDHPV
                     ISLDIHPTILAAAGVEPAADARLDGVNLLPYLKGEKTERPHETLFWRFGNQWAVRSGD
                     WKLLKAGKDQSPMLFNLKDDVSEQSDLAAKEPERVSTLRGVYEEWNRQLASPKWESGR
                     RRAGAQSRPRNR"
     gene            17309..18526
                     /locus_tag="P5316_06745"
     CDS             17309..18526
                     /locus_tag="P5316_06745"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HRR84691.1"
                     /translation="MLSTRSVLTQLIIVPLHVLSAMGETGGVASATTAPENSTNLPDA
                     KSYWVYVGTYTSPATGSKGIYLLELDMRSGEIKPMGLAAEVASPSFLALHPNRRFLYA
                     VGELNEFQGKKAGAVSAFAIDARTGKLTLLNQQPSGGQGPCHVTVDPTGKCVLTANYT
                     TGTVASLPIDNEGRLGEPTSIIQHIGSGPNAKRQQSPHAHSINLDRAGRFAFAADLGA
                     DKVFVYRFTANEGSLVANDPPFAQIAPGSGPRHFTFHPTGRFAYVINELASTITAFSY
                     DAERGVLKELQTIPTLPEGFAGENFTADIHVHPNGRFLYGSNRGHDSIAIFAIDSDNG
                     RLSAVGHEPTHGNWPRNFAVDSSGRWLLAANQKSNNVVEFAINPQTGLLAPTGRVLAT
                     PSPVCLMCVRAFP"
     gene            complement(18542..20017)
                     /gene="rpoN"
                     /locus_tag="P5316_06750"
     CDS             complement(18542..20017)
                     /gene="rpoN"
                     /locus_tag="P5316_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRR84692.1"
                     /translation="MSQFMSQSLSLGLRQEQRLTPQLIQSMNILQLPILALEAKIQEE
                     MERNPALEEVDLAPPGDLDGQPPVEPSSEGSTPVDAEQRHEAKGFEHLERLSREYDFD
                     DSDRPFGRRAGHGDGERDAKMDAMANTASRGISLNEYLRQQWAFVELSDHLKQAGELI
                     INYIDEDGYLRTPLEQIAEQHVPPFSPADMAEALSWVQTLDPPGIAARDLRECLLLQL
                     DNLEGNHELERILVERHLTDIEKNRYPEVAKATGRSIEDIKQAVRNLGRLYPRPGALV
                     VDRDVPPITPDVIVDYSEETGGYRVRLARGNSPKLRISETYRRMLADQNNDKDTRDFI
                     RKNLESAGALIDAIQYRRNRLQEVSEEIVDRQKDFFEMGPAGLKVMRMSDLAEKLGCD
                     PSTISRTVADKYMQTPRGIFPLRCFFTGGIETQTGEETSWDSVRARVKEIVDKEDKNQ
                     PLSDDAIAAKLQAEGITVSRRTIAKYRQQLRIPTARQRKQF"
     gene            complement(20367..21650)
                     /locus_tag="P5316_06755"
     CDS             complement(20367..21650)
                     /locus_tag="P5316_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRR84693.1"
                     /translation="MHQYRRVFPISTLFLDVLLCVGFVPSWAAAAPAAAKDAAVQNGV
                     DVLIADDFSLLAGKRVGLITNPTGVTRDLRSTIEVLHKSGKVKLVALFGLEHGVRGNV
                     HAGDKINDSNDPATGLPVYSLYGKTPKPTTQMLKGIDALVYDVQDIGCRSYTYVSSMA
                     DAMEAAAENGVGFVVLDRPNPLTGNRIEGRPLDLKYKSHVGYFPIPYIYGMTCGELAK
                     MINGEGWLAGGIKCDLTVVPLKGWRRDMWFDQTGLLWVPTSPHVPRADTSMFYAATGI
                     MGELQVVSEGVGYTLPFELAGGPFVENAEAFADELTRRKLPGVQFRPTYFTPYYMDHV
                     KGKACGGVQIYITDRDSVDLTAIQFHVMDAVRKLYPQVQLFGGKRDGMFDKVCGTDRI
                     RKMFLEGKSADEIVKYWNSGRDSFMKQRAKYLIYR"
     gene            complement(21707..22801)
                     /locus_tag="P5316_06760"
     CDS             complement(21707..22801)
                     /locus_tag="P5316_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR84694.1"
                     /translation="MDDPLAHLPEPQPDVERLKTVLRRGCPDRIPLFELAIAEEVLAE
                     LNAGSLLPPPADGEKSRLRAWAEQRVRLWWRLGYDYYRVRVNIPFTVDYGQASEGAPA
                     AVVGRQWVNEQQGLIQTRRDIERYPWPSPDSFGFEQAEAAIACLPEGMEAIGFSGGVL
                     EWAGTLMGLEHFMIMLHEEPDLVKEVVDRVGSLLLAAFERFCTMPEVFALWLGDDMGF
                     KTATLISPDHLREYILPWHRRFAELAHRTGRFFLLHSCGYVESVMPDLIEYVGIDGKH
                     SFEDAIVPVEEFKGRWGDRVAVLGGLDVDLLSRAPREVVCRRTGEILERCSPGGGYAC
                     GSGNSVTNYVPADNFLAMVETVHRFNGRMS"
     gene            complement(22801..23805)
                     /locus_tag="P5316_06765"
     CDS             complement(22801..23805)
                     /locus_tag="P5316_06765"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRR84695.1"
                     /translation="MTGNLRALFAAFAIVVLSGLCGCSPTRYGQRPGDPLMRCGDEIV
                     AAGRFFHTGTPVVLWMDPGGYDAYRCRRHFEPELIMPHTPAAPLDPNRYGTRRNLPKD
                     VEERIKDRGWSLDEARRVVDQFVIHYDACGTSARCFKVLHDLRGLSVHFLLDIDGTVY
                     QTLDLKERAWHAGTANDRSLGVEIAHIGAYGDMKTLDEWYAKDLFGWPYVSPPKWVKE
                     RRVRTPWFMGRAARKEVVTGNINGRDLMQYDFTCEQYDALIKLTAALNRIFPRLRLDY
                     PRGNDGRVRTEVFSEQELAAWSGLLGHMHVTKEKIDPGPAFDWDRVVEGARRERGTTW
                     "
     gene            complement(23835..25349)
                     /locus_tag="P5316_06770"
     CDS             complement(23835..25349)
                     /locus_tag="P5316_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRR84696.1"
                     /translation="MIWMSMLLSALAAAGQTDAGPLSGAAQLFTSDEPIMVRVRQLVA
                     DGRLREAENLLQAEQKIDDPRRSQAAADALEIIRRIRRDYRLELPALVERLRSPIPDV
                     TAADVERWRAAGEVQYRVLDGRPCYFIREPSNIFRFCEEARQRRDAHAATQAAPAPDK
                     KAQAEQQMIEHIRKALAAADASGKTEVLPIRQRIRYKLTVAPNRPGARSGSLVRCWLP
                     FPQEYRQQKQVRLIRTAPTECKLAPNAEGNQILTGAAQRTVYLEQKISDPAQPVVFEE
                     EFEYVCYAFCPKLDDAAARPLPDDWGKACLEERPPHVVFTPELREAVKQAVGDETNPL
                     AQARKIFYYINTRMKYCAEEEYSIIPSCTRKGLATWRGDCGIHAMLFIAMCRSVGIPA
                     RWQSGWETQPLEWNMHDWAEFYVEPWGWLPADASYGLKKSDDPRIRDFFFGHMDSYRM
                     IVNLDYGWQLDPPKHSLRSEPADFQRGEVEIDGRNLYYDEWDWDFSFDLQPVDD"
BASE COUNT         4993 a         7754 c         7919 g         5009 t
ORIGIN      
        1 tccaatagct gacgcggaag ctgccgccgc tgttcgccgg catgcttccg tccggattcc
       61 aggtcgggtt gttgtaggcc gtatcggtca gcaaatagag gcggttgcca tgggcgtaat
      121 ccccggtctt gatggagtag acgtagaggt ccatttccca ggtcacgtcg taggtgcccg
      181 cgggaagtgc tgacgggaat tgcttctgaa tccaggcgta ccggataccg tccctaatga
      241 ggttcgaggc gtagccgacg gacaccgagc ccgggtaacc ggtattcggg ccgtagatac
      301 cggcgccgtc gggcgcagga aagaccgtcg ggctgtcatt gtccacccca tcgttctgca
      361 gcctgcgata gacataccaa tcgaattcga gtcgcatggt cccggaagtg ccggggggca
      421 gggcgttggg gaacaaccgc ttcatccaag cgtgcccggt cttgtctttg aaatactcgg
      481 cggacgaata tcctgccgac tgtgatcccg gattatcgat gttcttgtag agcagcggat
      541 cgttcgcggc aaagagcttg ggttcatcgt tattggagac cggctcgcga caactgatca
      601 cctgggtcca gtcggcgagc ggatcggggc catcgaagtt gtcgtggaac tcgaacacca
      661 cctgctcgct tgcgtccctg atcacaaggt caacgtcgtc ccaggcgacg gtctcgaggc
      721 cgctgcactt ttcgtgcttg aggagacgca gttcgaggtt tccagtggtg gtggtcaccg
      781 tggcctccca ggtcacgcgg cgccacttgc cgttgtagat ccaggcggtg ggcgtggccc
      841 cggcattctg gcagtcggtg gtgacggtgc aaggctgctc gccgcttccg ctggccgggc
      901 cgacgcatcg gagctcgccg cccacgtcct gacaggtggc gtagttctca tagccgggcc
      961 agtagtcgac tttgaacccg ggcgcctcga ggttctcgtg attgtcgaag ttgaagcagg
     1021 ggtagtcgaa gtcttcgtcc gtcagcagat agactcgatt gcccacttcc cagcgaccgc
     1081 cgcagagggt tgcggggtcc gcgtacacat agaagtcata ctccaatgtc acgctgtacg
     1141 ttcccggggc taaggctccc ggccactgct tctggatcca gtggtccgag gttccgtccc
     1201 atgtcttgct ggactgaccc acgcactggc tgttgcccca gacgccccag tcgtctgaca
     1261 tggggccgaa catgttctgg tcccttggat cgctcgaaat aaccgcgccg cttccgtcga
     1321 acacgttcat tcgatcgggg taatcgtcga catacggggt ccttcccgtc gtccacgcat
     1381 ccagtccgct ctcgaagtcc tccgagagca cctcgatcac ttcacccgct tgcgcgaccg
     1441 cagggatcgc cagcagagcc attaacacag gaagcattct tctcattggt ccaacctcct
     1501 ctaccaaaat gttgacaatc tgtccggctc ggagcctgtc cccgacgcga agaccgagcc
     1561 tcccaagcct gtttctgttc ggaacgtcct ttacgcgata gcagcgcctc atacgcaggc
     1621 aatgcggttt ttcgcaccgt tttgtccggc tgttatcctc cttcccgcac tgtcacgaac
     1681 tgcgcgaggt ccgaaggcct cactgcaaat ccgggtcgag cgcggcgatc gtcgccgcat
     1741 tcttcacgcc caagagcctg agccaatagg cgtactgtgc tatatgatcc ggctggcccg
     1801 taggcagcga gcgttgggcc agcggcaccc ccgcggcgtg cccgtccatg aacaccgcgt
     1861 tggtgaaccg acccagatgc atgtttctgg ctgcccgccg cgggcccggc gcgtcggaag
     1921 tgttgatgtt attcttgccc tgcggcacat gaagtccccg ccatacatcc atcacaccca
     1981 tccaccactc ggtggcctgg ttggtacgat aattctgatg agcctgggcg agtgagatcg
     2041 cgcccacgga cgccgtgttg cgatcctccc gctcggcatc caacagatac acgacctcgc
     2101 ccgggctgcg gtaggtatta gtccggcagc gaggtgccgt tgatgcctgg aaatgatata
     2161 cttggtacca ctctccggtt gggttgggtg tgccggtttc gcctcctgct cgggggccgt
     2221 cggggtccat ggagctgacg acgtaatcca ggaacgggta atccatcgac aacatccgct
     2281 ccgggcaatg cagcaactcg atttccgcga ccctgagctg attgacggta aagcctgcgg
     2341 gaattcgctt gatagctccg attgacctgg ccgcgaccac ggcccagttg gtcggatcga
     2401 aatcattgca tgcccgcggg acgatgtcgg cgttctggac ggcatactgc tgcacggcca
     2461 taccgatctg tctgaggttg ttcaggcagg caacgctgcg ggcctgtgct ctcgcccggc
     2521 tcagggacgg taacagtatt gccaccaaca aagcaatgat cgcgacgacg acgaggactt
     2581 cgatcagggt gaagccacaa actggcggcg tttttttgca tgtccgttgc attgcgcact
     2641 tcctccacca cgatgggcga cggtcgctct tacaggtgca ttgcgcctac tccgacaggc
     2701 tctcacacat atctcaatgt atcacagccc tagtgatgaa tcaaggccga catagaaaaa
     2761 atcgcatgag ttgtcagagt cccgctcatg cggacatggg tgaacagcag aggttggtta
     2821 agaggagggc gaagacccga tgcggaatag gtcgggttta tgtttctgaa aaatcgctgg
     2881 aacacgccca ggctgagtgg tcaacgaggc ggcgctccac gatcggttac ctgtttcccc
     2941 aggcagccga ttgttcttat ggcagacaca acccggggaa ggagcaagcg gttgtctttg
     3001 ctctggatct gccgggagcg accgatgtgt cttaatcggc acaatcccgc tcggccggca
     3061 cgtcagctcc gctcagacag ccgcggaaga tctcgatgtc aacggcatcg acatcgccgt
     3121 cgtggtccag atccgcacgg acgcagatgc cggagacatt cgttccattt ccactgagac
     3181 aaccctgttc ggtgccgaag tccgcctggt cgacgtcgta gtcggcgtcg aagtcgcccg
     3241 gttggtactc aacggagatc cggaggctgc tggtctcgcg ctcgccgtcg ttatcgatga
     3301 ccgtcaacga cgcgagatgg tcgccaacgc tggtgtatgt atgagtggca ctcggtccgg
     3361 ttgcatcgac ggttccgtca gtctcgaaat cccaatgata ctcgacgatg aaacccgccg
     3421 ggtcggtgtc ataggatgcg gagccgtcga agttcacggt gagcggtgct tttccggacg
     3481 tgggatcggc agcgatcacc gcgacggggc agccgccttt gaccagttcg atgccgttgc
     3541 cggtgcccag gttccggctg tattggcggt cccgagggtt gttccaatgc tcatggacgc
     3601 cgagactggc cattcgcacg gtgggggtgg gatgatcggg gtcgtagaag gtgcctgaaa
     3661 gtggattgtc ggccaaggct ccttcatgca ggtaatcatc ggccccggac atatgggggt
     3721 agtcggtcca ctcggcccaa aggaagtcaa agcaaaccga atcgatggcg atgggatcct
     3781 gggagatgaa caggctggag gtccaatcac cgttgaaggg atcggtgtac catttgcgcg
     3841 gggcaacctc gatcgggtgc acacccgcat agaggccgtc gatgaggtag agaagggcct
     3901 tgccgccggt atgggcgtgg cccatgaggt cgaccatgca tctgtagcgt cccatgcctg
     3961 tgacatactc gggcaagcta tcgtgcaagt tgtaataacc gccttcggtc ggcagtcgca
     4021 ggaagccgta gtagtttttt ccgcacagtg tgacccctgc ggaagtatgg cttttcaagt
     4081 tcgccaagtt gatgaagtag gcagcctcgg cataaggggc cagaatcttg tcctgcgtca
     4141 ccccgctcgg gcgagagctg aaatagaaat tacggtcgac gcctgcggct tggttcggcc
     4201 aaacttgccc agatgatcga gataccgcac attggggaac tcgtcgtgca agaggttgta
     4261 gtattcgttc gcgaacaggc agaccggatc tcctatcgag atgtccgcct ggttgacgcc
     4321 gacgacattc acgagttggc ggagcagtgc cagcatcatc tgcggagacg tgttcatgta
     4381 atcctgtttg ctgacgagat tgtaggtatt gggatcgacg ccgccccatc cgcccatgtc
     4441 gataaagccg acaaaattga ccttgatggc cactttctcg cccggggtgt atccgacgtt
     4501 gccgcgaccg tgggtcacgt tgaaatgacg gatcagggcg tcccaagcgg ccgtgtcgga
     4561 ggtctggccg gtcagggaac gaatggctcg cgacatcatg gcgtcaacga cggtctgatt
     4621 ggtgtgagct ggttcccacc agtggccata gcccggcccc acccaatcgg tcgcatcagg
     4681 atcatggacc cagacgaccc gaccggggaa gatgcctttg gcttcgccga tgggagcatt
     4741 gggctctgga ttggccgcct ccgcaaaccg atccggcgtg taataaaccg caatgacgcc
     4801 tgcgaccgca gccaccgcga aacaagcgag cgcgagctta agttgtgctc gcccgagcag
     4861 tgcgcgggcc ttgcgataag caagtgccga ggtaatggcg ccgacgatcc acagaacgaa
     4921 gccccccgcc atcggtgcgg cgatgcgctg gcacggatag gtcgcccgta acggcttggg
     4981 cacgacgcga gcgaggaacc agatcaagga gagcaagccg gcgaccggga acaaccactg
     5041 ccagcgaagg tgtctgggac ctgcgggccg atgcgcgttt gggggacagg gagatgcggg
     5101 cgggcaggct ctcggatcag tcatgtgtca ttccctcgtg agctgggtgt cggtccaagc
     5161 gttctgtgcg atccgcgacg aatgagcaaa gtatagtaga gtattctacc atgattgcag
     5221 ccggccgcac tagtggggtc gtacttgcgt catttcgtcc ggcgccttgc gtctcgaaga
     5281 ccatgtgccg cctgccgcgc gtgccgtcct gaaaagcggc acttgatttt gcacggcgcc
     5341 ccgactccga ggcactgtgg gaagcgtcga gaccggtctc agatcatccg gcgcagtggg
     5401 ggtctgagta ggcacctggt ccgctgatgc agcgttggag gaggccgaaa tcggtctgat
     5461 caacgtcctg atcggcgtcg atgtcggcca actcgcaagc tgttatcggc gggccgttac
     5521 cgctgccggt cacgcactca cggaacaagg caacatcgtc ctggtcgaca ctcgtgtcgt
     5581 cgtcaagatc cccgacccgc agtactctga ccgtaacctg gtcaatagcg gatcggatcc
     5641 cgtcgtaggc catcaactgc aacacatatg tgccgacggt cgagaacgtc gccagagtgc
     5701 gggctgccgt gggggccgac caatccacat cccccgggcc gctgactttc gaccatgcca
     5761 ccgtgagtgc tccggggggg tcgggcaatc catcatcggt gacgctgccg tcgagccatg
     5821 cggtggggaa tcgcgaggtg gttcgcaaat cgggtcccgc gttgacggca ggcggctgat
     5881 tatcttcggg cacgttgtgc cagcgtccgc cgcggacctc catggccaga ctgtacagct
     5941 tgccgtcgca ggtgatatac aacgtgcggc cgtcggtgcc gccgaaagtc aggttggtga
     6001 cgaactcggg cacagcgacc atatcgagct gctgaccgtc gggcgagacg atccagaaac
     6061 cgccccggcc ggcaaagtag aggttgccga actcgtcaat ggtcataccg tcggggtcgt
     6121 tctggttggg cgtcgagggg ttgaaaaaca acgagccggc accgacgctg ccatcgggat
     6181 tgacggggta cgaccgccac cacttcctgg agctgtcgga aacgtagagg atgctcccgt
     6241 caagcgaacc gatgatacca ttgcacaagg gcatatcggt aatcacggcg gttgtggcgc
     6301 ccgtggaggt gagacgatag accttgctgg tcgtctgagc accgaagtcc ggagtggtga
     6361 agtagatgtc gcctgcgggc gtctggcaca agtcgttcgg ggcattccat acggggttct
     6421 cggccaggat gatggggtct tccgggccgt tgggtccgat tcggaggctg agaatgcgcc
     6481 tggcgctgcc ctgggcgcag agaaggcgtc catcgttggc gaggtaggtt ccgttaatcc
     6541 cacgagtgtc actcatccag agcgcgctcg tgtccggcgg agtcaggcgg aacagctcat
     6601 tcgtggacaa cgccgagaag taaagctttt ccgtgaacgt gtcccaggag gggccttcat
     6661 agaagcgtga atcactgcga acgggaacca gggtggcccc cggcgcgatc aggccgccgg
     6721 aggccggggt ccggccgacc gtgcccagca gaacgacaag gactgcgagc acagccggcg
     6781 gctttccaga ccacccgccc aaggaccggc ttggatgcat cgacaaacaa cctcagaaaa
     6841 agcctgacgg tgtcggggag aaccacatgt tgcgcggctc ctgcaccccg gacctgcgat
     6901 actgattata ccatccggcg acgtattctc ccgattgttt cgtccacgag cccgtgtcaa
     6961 gtccgtgtcg gctcacaagg gtaccggcag gcgctcgcct gcttgagtcc cgcaggcgta
     7021 tggtggccgc gtggtcagac cgggtgccgt gccgcgggcg accggcggac gtctacgggc
     7081 aactggtcgt ctcgcaggtc gtcccgacgc cctggggaac cccatccagg ttcctgcatg
     7141 tccccggaag gatctcgaca cactttccct ccgtcaggca gcacgcttcc atcgaacact
     7201 gcggattcgc cgggactccc gggccgctgg cgcactgctc gaatacgagc aggtcggctt
     7261 gatcgatgtc cacgccatcg gcgtcgagat cggcgcacgt gcagtccgga tcatcggggg
     7321 gcatcggtat accaccgtcc gaatcggtgt aacagagctg catgagggca aagtccctct
     7381 gatccacatc ggcgtcgcgg tccaagtctc cgcggaccgg accgcaacaa ttgccgttca
     7441 ccgtgatctc aaaggtcttg gtggcggtgg cggacgcgcc gtcttccagt tgcacagtaa
     7501 aggcaaaggt tcccgccgta ttcggctgac cggagatcag cccgttgggc gacagcttca
     7561 ggcccggagg cagattcccg gaagcgacgg agaacctcag cggctcaacg tagcagccga
     7621 taagctgaag gttgtagagg ctgtcgcaaa ccgctgccgg caagggcgac tgggtgacga
     7681 ttcgcacgtc ggtcggaacg acagcctcgg cgtagaagtc gaccatgtca taaaagtggc
     7741 tgaagggcag cgtggcagtg gaggacaggg tgctgttgcg caagagaatg tactgcggac
     7801 aagaggcggc cccattgaga tccgtgggcg agaattcgcc cgtccgctcg atctcgacaa
     7861 agcccttggt ggcacagacg tcgccgatga gcgcggtgct gaaatcgatg atcttcatcc
     7921 agggcacctg atacgactgc aacaagtcga agttgagcgc atcggtgggt tgttcgagga
     7981 tggaacgagt agccgggttg gggtttccgg cctgcttggc ccaagccgtg tccccccagc
     8041 acaactccat gatctggctg ccggcgttgt ctttcatcag cgtcaggtac cgcaatttga
     8101 acctcaaccc ggtggcgcac ggcgaccagt tgcggatagg tacaacgtgc ccccaatacc
     8161 cttgtacacc cgtgccgttt tcgcccccag ggttagcccg gccccagaag tgagtcccgg
     8221 aaggagtgaa ggccccgagc gggtcggtgc catggggaag cggcggaggt tctgtgccgg
     8281 gattggtgta tccctcaacg gggccccgcg tggcgccgtc ccggccccag tcgcgcagat
     8341 aattcctgta tccctcgggc ggcggaggcg ggcgatcgcc ccagccactg ggcgggtcgt
     8401 atcgaatacc cgttttgtcc ccgtggtgtt gggtgttata cggggggtct tcgaagtcgc
     8461 tgttgggaat atccccctcg aatttgccca tgaggctgcc cgtcgatgcc gtggccgaga
     8521 aaaccactcc gtcaaagagg ttgtgcaggt tggtcaacct ggaatcagtt ttgagtctga
     8581 tctccagcac caccaccttg gggtttcccg ggacactgcc gctgagcttg atcttccgcc
     8641 agttggtgaa gcattggttc acttcgtttg catcgttgcg tgtcacgaaa aacgtagtct
     8701 tcagttgcgt cagcaccacg cgtttcatgg ggacgttctc gtcgtactgt ttgtcctggt
     8761 tggtttcata ctgcggcgtc cccggaaggg tggagaagtt ggtcacctcg atgacattgc
     8821 cggcatcatt cagattggtg ttggtggcgc cgttggcata ggtctctttc caaagcagga
     8881 ccaccacttt gctgccgacc gacgcggtgc agggagccga ggcatcgtcg ttaatggcca
     8941 tggccagctt ggtcttggcg ttggcgacac ctgcgccgcc acctgccgcg ctcacgtcga
     9001 ccagaacgtt ggcagggtcg cggagaacgc cgtcggtgga gaactcatag acacggttga
     9061 cgccgccggt gtcgatttcg atggtatcac catcggccac ggtgttcccg attcgcacga
     9121 atgccgtgtc gtggggcatt tctccatcca aggcccacca gaggatgtgc tcctgcctcg
     9181 tctcgtagcc ggtgtactgt gccgagtggg ccacctgagc ccaaagctcg agatcgtagt
     9241 tcaccctgtc tgcctcggga tgccagttct tcacctcgag gggctggcgg tagagcacgt
     9301 gcccttgaaa gtcgggcgtg gtacccttca ggtaggaaat gcccccgaaa tagcggccgt
     9361 cgggagctgt cagcccatgc ccgcacacgt cggtttccgc cggcgggtcg tgataccagg
     9421 tttggccggt gccccaggtt tgggcccaga tgatccagcc gttgtttgtg ccgtcctcga
     9481 agctgccgtt gaccagagat tcccctgcca tggataccgc gggcaacgcc aatagcagac
     9541 cagccagcga gataggaatg gatcgcatca gcataccctc cagtgagagg tgattgagac
     9601 aggtcatgcg gcccggccag acggcgttac cgcaacgatt caagtccgtg ccaggatgag
     9661 aatctgactc accacgaaca atggggttct gcggccgtga acgagcgcgc ccaacgtcgg
     9721 tgccctacac agccgttcat cccggcagac atgctgccgt caggttattg cattcttggg
     9781 gaaaaaatca agcctggcat ccgaaaaaca ctgccgagtt gacagaaaac tgctggtgtt
     9841 tttgccggca gatatcgagg ttcgggcgct ggggaggtga acaccccatc ctggccggac
     9901 ctgaccggaa atgatgagct attctgatcg ctacagggca agcgtcgtaa cctctcccgg
     9961 ctacaacaac gacgggtctg ccaagctgct gtcccgcgcc gagccgctgc ccgcgatgag
    10021 cacaacatgc gctgcctgga agaaggaccg cgagaagctt gaccactctt tgtcaactcg
    10081 atggaccttt tgctcgtcat gcatcggtcg acgctatcga tgagccatcc gatcatgcgt
    10141 caagcgaggc ttggaaacca tcacctttcg actcccggat cttcaggggg caccgatggg
    10201 gaaaaggccc tcgtgctgcc cgtctcctgg gcataagacc gagtctgcgg accgtggcgg
    10261 caagaggttc accaagtggc ggggcaagct atcggggaaa agagcagtct atgataagaa
    10321 catcttgacc cgggtgtttg cggaccggta gaatgcgcgc gtcatatcgg aagcacggcc
    10381 gtatcgagcg gacggcggac gggattggag agaaggcttc ttagttcatt tcaggaggac
    10441 tatggatgcg caaatcaatt cttgtgactg tattccctgt ggtgctgggc ctggtcagcc
    10501 attgggcagc cgggcaatgc gagttcgttg cgacaagcgt gacgccgaat attgcgggcc
    10561 gacaccttaa cccgccctat ttgtatgttg tcttcaaagc aagtgcgacg gatccgagca
    10621 ttcacgtgaa cgacggggat ctgatgacct ttgataccgg ggctcagttt ggcgggccgt
    10681 acgtcttcga gttcgacacg gacaattcgg tggcccccgg caacatagcg gttcctctga
    10741 cggccaccga caacttcacc agctcccggg cgctggtcga ggtcgtgaaa gcgcatccct
    10801 ctcttccgtt ctcggcgctg gtggcacctg taggtccgga cgatcaatac gtaagttttg
    10861 tcttcaagaa gaacgaaggt tacggcgcgg gggtatcaag gactcttgcc gtcccgggag
    10921 ccattacggg cagcgctacc agttttgcat atcagacctc cggcaacgtg tggttccaga
    10981 ttgcgggcag caatctgcat cccggcagcc cgcacatcgt ggccaagttc gtcagccagg
    11041 ccgatcccac caaggtcatc accaccacgt caaccatcag aacggcggag gtgttgaccg
    11101 tcagggcatc cttggggctg ggtgccactc cttttccggc gggggtctac gatctggtcc
    11161 tttcgcgagc caccactcct gcctgccccg acttcgtgct ccccggggct ctgacgatcg
    11221 tcgataacct gctcagtgac ccgacgttcc agacgaacat cctgggcaac gccgggacgc
    11281 tgatgtggaa cgttgagtac aacaccgctt ggctccctct ggtctggcac cacaataacg
    11341 tcaacatcac caattccttc gcagacggcg ttttctatga agccgtgaaa cgcggcgaca
    11401 agttccaccc catgcagcag gagaagccaa ccagaaagga agcaccgcgg caggctccat
    11461 tccccacggg tccattggag agcacggccg gggacaacga gtgggcgacg ctgcggtggg
    11521 atccggccaa cggcatcgga atcgggcggc atcagttctg gcagtacatc gacatcaatc
    11581 tgtcggagac gtcgttcgtc acgttgtcgg gtgtctggct cggcggcacc accagcccct
    11641 cgacgctgac gtacggcatc gagatccgcg acggcgacga gaacggcacc attctggccc
    11701 agacgccgcc gcaacagacc atcgacagtt tctattggac ccctttctcg tacgtcatga
    11761 cgctgccagc cggccagcga cgggtggcct gcgtgattta cggggataac tcggatgacg
    11821 tcgacgcggg gctgcatatt gatgacctgc tgttgcgagt cggcgcggag ttctggcccg
    11881 acgtcgacgg agacggggat attgaccagg cggacttcag cagcttccag gcctgctaca
    11941 cgggtgcggg cgcgggcgtt gtcgccgcgg gctgcgagcg attcgacagg gaagcagaca
    12001 tggatgtcga tgcggcggat ttcgaggcct ttgagagatg cgcgacgggt ccagggattc
    12061 ggctgacgcc tgttgctgtg gccgccgggt gtgaaccgta gagattcact tcgggcgacc
    12121 tggcaaggat cgcccacaaa tgaacgatct acgcgcggga gaacgtacgg ttcccaggtc
    12181 aagcggggcc agggatgttc cttgttgcgc ctggaggagt acaaggcggc agatcggttc
    12241 cgcgggtccg tgaacgcggc ccgtacagcc actcaacccg gttggaccat cggcaagccg
    12301 gtctggtagt ctatggggcc agtcggaaga aggaggggcg agatcgcgat tgaatcagcc
    12361 ggtgggctga tcaccgttcg ttcagcgtgc gtttgctgcg atcaggaggg ttgctgatga
    12421 gaagccgtct tctgccgctg atcaccctga ttctggcgaa tcacgcaggc gccgagacgc
    12481 tgaccctgcc gctggagcaa cgacctgcgt ggttggcccg tgagggcatc gtgatggccg
    12541 gcagttggga gcccctgcta ttccgcgtgc gccgtgacgg cagtgacggt tacacaccga
    12601 cgccgcagca ggtggccgac taccggcgag aacacagtcc ggaaatggtg gcgcgactca
    12661 agtcgcttgg cgtcaacttc gtcatgatgc attcgtacaa ggcattcggc cgtgaggcgg
    12721 agcgcgagag catgcaggac gcggtccgat ttgccaagct ctgccgcgag gccggcctgc
    12781 gggtcggagt ctacaacaac agcggcacgc tgggttggga gttgctcttc aaggaactcc
    12841 ccgaggcgaa ggactgggcg gttctcgacg ccgcaggcaa gccggtcacc tatggacggg
    12901 cgacctatcg atattactgg gaccgtaatc atccggccgc ccaggcattc cacaaggaga
    12961 tcatccgctt ctcggtggag cagattcaag ccgacctgct tcacttcgac aactacatca
    13021 tcgggccggg ccacaatgcc aactcggtga gccgttttcg cagctacttg aagagccgat
    13081 tcaagcctca acagcttgag gagatgggca tcacagaccc tgaccgtgcg atgcccccga
    13141 ccggacccga cgcaaacgga ttgcttggcc gggcctggct cgacttctct tgtgggtcga
    13201 tggcggagtc ctaccacgac ctgagccgct atgcgcgcac tctgcggtct gacgtactcg
    13261 tcgagtgtaa ccctggcgga cccggcaatc gcatctcacc gccgatcgat cacgggcggt
    13321 tgctggttgg cggggaggcc ttctgggatg aaggccgcga gccgggatac cgcgacgggc
    13381 agctcaacac ccggatccgc acctacaagc tggcccgccg catgaataac atcgcctttg
    13441 cctatacgac ctcgccgctg gaggccgccg aggcgatggc cttcaacctc gactgcttcg
    13501 gctgcatctg ctggttcgaa tacggccgtc tggccgccaa gccgggcgtc gagacccccg
    13561 tgtccctgga gttggcccca tatattcgct tcttccgcga ccgccgcgat ctcctgcgta
    13621 atgcggaggt cgtcgcagat gtggcaatcc tgcggagctt cccttcgcag gtctacgctg
    13681 aagcgacaca ggcccagctc accgcgcagg ccgagcaatt cctcatcgaa aaccgggttc
    13741 ccttccagat catctacgac gatcatctga gcgagttgtc gcgataccgc gccttggtcc
    13801 tggccggctg cgtggccatg tcggacgccc agatcgagca ggttcgcagc ttcgcggccg
    13861 gcggtggacg cgtgctgctg gtcggggatg cggcgaccca tgatgagtgg ctgatgcccc
    13921 gcagggcgcc cggcctccag gatctgccgg aagctcaatt ggtgcggatc cagaagatcg
    13981 ctgaaatggg ggacgcggtc agtacgctgt tgcaggcatc accctcattg gccgtggagg
    14041 ggcctcacgg actgtgtgcc gagctgaccg agcggcccgg ccgtcgcctg gtgcacctgg
    14101 tcaattaccg caccgacaca ccggcccgca acgtcaccgt gaccgtccag ctcccggaag
    14161 gcaagcaggt caagaacgtc cgcctggccc gccctgagca cgataaagac gcagacctgg
    14221 agattcgaca acaaggaagg gtcataacct gtgttgttcc tgtcatcgac gtgtacggga
    14281 tcgtcgtcgt gacccgggat acaccgggtg caccacgatg agtgccctcg actgcagctc
    14341 ccgcggctgt gcggtctgcg actgcgcttt cctttactgg agcccgtccc caggatgccg
    14401 atacacatta tgcacgcaat cgggttgact tttcctggcc agggtacata atgtactcat
    14461 gagacgcgtg atcagcattc ggcgactgcg ggagttctgg aaagagcacc cggatgccga
    14521 gacgccgctt cgggagtggt acaagattgc tcgaaaggcc caatggcaca gcctccagga
    14581 cgtccgcaag acctacccga aagctgacgc cgtgacagcg ggcagcggag caacgatgac
    14641 ggtgttcaac atcggaggca acaagttccg gctgatcacg agcatctggt atcccggtca
    14701 gcagatctat atcaagatgg tgttgaccca tgccgagcac agcaaggaca agtggaagaa
    14761 gcaactgtga tcgccgtgga ggatgacgat atgcctcgaa ctacaatatc tcgatctccc
    14821 cgcaagctgc cgggccggtt tgaagacctg gtggcgttgc tggcccctcg ggccatccgt
    14881 gatgagatcg attacgaaag catgattgag atgctcgacc gccttacctc cctgccccgc
    14941 ctgacaaagg ggcaggagca gtatttcgag acgctttccg ttttggtcga agcctacgag
    15001 aatgaccatc acgccatcga ggtggctgac ctgcccccga tccaggtgct caagcatctg
    15061 ctcgacagca gcgatatgaa cgcctcccag ttgggcgagc tgctggggaa tcgctcactc
    15121 ggatccaaga ttctgcgcgg ccaaagggaa ctcagcaaga accacattcg tttgttggcc
    15181 gagcggttca acgttgaccc cggtcttttc atctgacttg tcgggtgccc cacgtgcttc
    15241 atccaggcag gagagcgatc atcggttcac caaacaaact ggcagtccgt tcttccgaac
    15301 agaagacgac gggtggccta ttcccgccgc ggcgggggca aggtcgcgat gatcgaatgc
    15361 cgcacggccc ggccctcggg taagtcggac ggctacgcct ccaacagcca tgcagctcgg
    15421 tgaataccga tgtctgctca gggtgccgac ctgccccaag cctccttgac acgcccgtga
    15481 gagcggacga cgatgaccga ggggcggata tctggagcga atgtgtcaag agccgagaat
    15541 tcgagcccgg tttggccatc gaatcttcga ttggcccgca caggagaccg tcaatgacaa
    15601 ctgccgacag agcatcgacc atctggctga ccgcattgag tgtcgtcatg cccgttgccg
    15661 caactcaggc tgcccaagcc gagcccgccg gcaaggccgt gaagcggccg aacatcgtgc
    15721 tgatcgtctc agacgatcag ggatacaacg atatcggcat tcagggctgc gaggacatcc
    15781 cgactccgaa catggacgct tttgctcgtg ggggcgtccg gttcacgaac ggatacgtct
    15841 cgtgtccggt gtgcgccccg acgcgggccg gcctgatgac cggccgttac cagcagcggt
    15901 tcgggttcga gttcaacccc ggacctgagg gccaggcgtc cgatcgtttc ggtttgccgt
    15961 tgtctgaaaa gacgattgcc gagcgcctca agcccgccgg ctatgtcacc gggatggtcg
    16021 gcaagtggca cctgggcttc aaggacgaca tgcagccgcc gcagcgcggt ttcgacgagt
    16081 ttttcggctt cctggccggt gctcatcaat acctttcccg cgacggagga gctgctgctc
    16141 caatcttacg tggccaggag caggtcgatg agaaggaata cctcaccgat gccttcaagc
    16201 gggaggcgct ggcgttcatc gatcggcaca agaacgagac cttcttcctc tacgtgccgt
    16261 tcaacgccgt tcacgcgccg ctgcaggcca cggacaagta tctgaagcga ttggacggca
    16321 tcagcgacga gactcgtcgg attcacgcgg cgatggtctc ggccttggac gacgccgtcg
    16381 gcgaaatcat gaccaagctg cgagacagcg gcgtcgaaga gaataccctg gtattctacc
    16441 tgagcgacaa cggcggcccg acccgacaga ccacatccag caacaaaccg ttgcggggat
    16501 tcaagggcga tgtcttcgag ggcggcatac gcgtgccgtt catggtgagt tggaagggcc
    16561 gtctgcccgc gggcaaagtt tatgaccatc cggtcatctc gctggacatc cacccgacca
    16621 ttctggccgc ggccggggtg gagcctgccg ccgacgccag gctcgatggc gtcaatctgc
    16681 tcccctacct caagggcgaa aagacggagc gcccgcacga aaccctcttc tggcgattcg
    16741 gcaatcaatg ggcggtccgc agcggcgact ggaaactgct gaaggcgggc aaggaccaga
    16801 gtccgatgct cttcaatctc aaggatgacg tcagcgaaca gagtgacctc gccgccaagg
    16861 agccagaacg ggtcagcacc ctccgtggcg tctacgagga atggaatagg cagttggcgt
    16921 cgccgaaatg ggagagcgga cgcagacgcg cgggagcaca gagccgaccc agaaacaggt
    16981 aatctcggag agcgctgtgc gcacctgaaa tcagtccctt ccgtcgcgac gggtgtaggt
    17041 ttttcgcgca tgttgcgtgg tgagaatcca cggcagaccg tctttttacc ggccgcaacc
    17101 aaagcgcacg ggaagacaga aacatacgcg caacgtgcgt attagttacg acggaaaaga
    17161 ccatcaaaaa aacgctcttg ccaatcccac tgggcggcgt gaaacccttg cggtggcgaa
    17221 acgttcattt ggaatgccgc gtcgctttgc tgttccgtcc cattcgggat attatcgccc
    17281 tcgcatcttc cgaaatgtga ggtcgatcat gttaagcaca cgatctgtat tgacgcagct
    17341 cattatcgtg ccccttcacg tgttgtctgc catgggcgaa accggcggag ttgcctcagc
    17401 tacaactgca ccggagaaca gcacaaacct gcccgacgcg aaaagctact gggtctatgt
    17461 cggcacatac acctcgcccg ccaccggcag caagggtatc tacctgctcg aactcgacat
    17521 gcgatccggt gagatcaagc ctatgggcct ggccgcagag gtggccagcc cgtccttcct
    17581 ggcgctgcac ccaaaccgcc gctttctgta tgccgtcggc gagttgaatg agtttcaggg
    17641 caagaaggcc ggcgcagtca gcgcctttgc catcgatgcc cggaccggca agctgacatt
    17701 gctgaaccag caaccctccg gcggccaggg accctgccac gtcaccgtcg atcccaccgg
    17761 caagtgcgtg ctgaccgcca actataccac cgggaccgtg gcctcgttgc cgatcgacaa
    17821 cgagggccga ctgggtgaac cgacctcaat catccagcac atcggatccg gccccaacgc
    17881 caagcgtcaa caaagccctc acgcacattc gatcaacctg gaccgggccg ggcggttcgc
    17941 attcgccgct gatttggggg cagacaaggt cttcgtctac cggttcacgg caaatgaagg
    18001 atcgctggtc gccaacgatc cgccctttgc tcaaatcgcg ccgggctccg gaccgcgaca
    18061 ctttacgttc cacccgacag gtcgctttgc ctatgtgatc aacgaattgg cctcgacgat
    18121 caccgctttc tcatatgacg ccgagcgagg ggtcctcaag gagctgcaga cgatcccgac
    18181 gctgcccgaa ggcttcgccg gcgagaattt cacggccgac atccacgtcc atcccaacgg
    18241 cagatttctc tatgggtcga atcggggtca tgacagcatc gctatcttcg caatcgactc
    18301 cgataacggc cgattgtccg ccgtaggcca cgaacccacg cacgggaact ggccgcgcaa
    18361 cttcgccgtc gactcttcgg gcaggtggct cctggcggcc aaccagaaat cgaacaacgt
    18421 cgtcgaattc gcgatcaatc cgcagacggg cttgctcgcg ccgacgggtc gcgtgctggc
    18481 aacaccgtcg ccggtatgcc tcatgtgcgt cagggcgttt ccatgattga aagagccgcg
    18541 atcagaactg ttttcgctga cgtgccgtgg gaatacggag ttgctgacga tacttggcga
    18601 tggttctgcg agacacggtg attccctccg cctgaagctt ggccgcgatg gcgtcgtcgc
    18661 taaggggctg gttcttgtct tctttgtcga cgatttcttt gacgcgggcg cgcacgctgt
    18721 cccaactcgt ctcctcaccg gtctgtgtct cgatgccgcc ggtgaagaaa caccttaagg
    18781 gaaagatgcc acggggcgtc tgcatgtatt tgtcggccac ggtgcggctg atggtcgaag
    18841 gatcgcaccc gagtttctcg gccagatcgc tcatccgcat gactttgagc cccgccggcc
    18901 ccatctcgaa gaaatccttc tggcgatcaa caatctcctc agacacctcc tgcaagcggt
    18961 ttcgacgata ctgaatcgca tcgatcaaag ccccggccga ttcgaggttc ttgcgaatga
    19021 aatcccgcgt gtctttgtcg ttgttctggt ccgccagcat acggcggtag gtctcggaga
    19081 ttcggagttt cggactgtta cctctggcga gccgcacgcg gtagccgccg gtttcttcgg
    19141 agtagtccac gatgacgtcg ggcgtgatgg gcggaacgtc gcgatcgacg acgagcgcgc
    19201 cgggccgagg gtaaagccgc ccaaggtttc gcaccgcctg cttgatatct tcgatacttc
    19261 ggccggtggc cttggcaacc tccggatatc tgttcttttc gatgtcggtc agatgccgtt
    19321 cgaccaggat gcgttcgagt tcgtggttgc cttccagatt gtccagttgc agcaagagac
    19381 actcgcgcag gtccctcgcc gcgatgcccg gcggatcgag ggtctggacc cagctcaggg
    19441 cctcggccat gtcggccggt gaaaaaggcg gcacatgctg ctcggcgatt tgctccagcg
    19501 gcgttctgag atatccgtct tcgtcgatgt agttgatgat cagttcgccc gcctgcttca
    19561 ggtgatcgct gagctcgacg aaagcccatt gctggcgaag gtactcgttg agcgagatac
    19621 cccggctggc cgtattggcc atcgcatcca ttttggcgtc gcgttcgcca tcgccatgcc
    19681 cggcccggcg tccgaacggt cggtcgctgt cgtcgaaatc gtactcccgg gaaagccgct
    19741 ccagatgctc gaagcccttc gcttcgtgcc tctgctcagc gtcaactggg gtgctcccct
    19801 cgctggacgg ctcgacgggg ggttggccgt cgaggtcccc tggcggagcc agatcgacct
    19861 cctccagcgc gggattgcgc tccatctctt cctggatctt cgcctccagc gccaagatcg
    19921 gaagctggag gatgttcatc gactggatga gctggggagt aagccgctgc tcctgccgaa
    19981 gacccaaaga cagactttgc gacataaact gcgacatcga cagatattat acccgataga
    20041 tggccgggtc gcagcatgcg accgcacggt cgatccgact ggcgggcgac cgccagcacc
    20101 cggcttgcgg cggcgatgac ggccgaggca cggccagaag cattgctcag acacgcgcca
    20161 gcgcgttttg ggcaggtcca gacgtactgg ggcaagccat cagtggcaac ccgccccggt
    20221 gcaagccggc gttggcagtt gcgctgagac tcctgggggc gtcgtgtgac ggtcgagtgg
    20281 gcggtgcctt gcgtgcctgc aacggcgcac cacgtagcga aaagcagggg gcgcgacggc
    20341 aggaccctgg gcgaaaccgg agggcttcat ctgtagatta agtacttcgc ccgctgcttc
    20401 atgaatgaat cgcggccgga gttccagtac ttcacgatct catcagcaga cttgccctcc
    20461 aggaacatct tgcggatccg gtcggttccg cagactttgt cgaacatgcc gtcccgcttg
    20521 ccaccgaaca gttggacctg cggatagagc ttgcggaccg cgtccatgac atggaactga
    20581 atcgctgtga ggtccaccga gtcgcggtcg gtgatgtaga tctggacgcc gccgcaggcc
    20641 ttgcccttaa catgatccat gtagtacggt gtgaagtagg tcggccggaa ctgcacacca
    20701 ggaagcttgc ggcgagtcaa ctcatcggca aaggcctcgg cattctccac gaacggtccg
    20761 ccggccaact caaacggcag cgtgtaaccg acgccctcgc tgacgacttg caactcgccc
    20821 ataatgcccg tcgcggcata gaacatcgac gtatccgcgc gcggaacgtg cggcgaggtg
    20881 gggacccaca acaaacccgt ctggtcgaac cacatgtcgc gtcgccagcc tttgagcggg
    20941 acgaccgtca ggtcgcactt gatgcctccc gccagccagc cctcgccgtt gatcattttc
    21001 gccagttcac cgcaggtcat gccgtagatg tatggaatag ggaagtaccc cacgtgggat
    21061 ttgtacttga gatcgagggg gcgtccttcg atgcgattgc cggtcagggg attagggcga
    21121 tcgagaacga cgaagccaac cccgttttcg gccgctgctt ccattgcatc ggccatggaa
    21181 ctgacgtagg tgtatgatcg acaaccgata tcctgaacgt catagacaag ggcgtcgata
    21241 cctttgagca tctgcgtggt cggctttggg gttttgccgt aaagcgagta gacgggcaga
    21301 ccggtagcgg ggtcgttcga gtcgttgatc ttatcgcccg cgtggacgtt gccgcggacg
    21361 ccgtgttcaa gcccaaacag ggcaaccaac tttaccttgc cggacttatg cagcacttcg
    21421 atggtgctgc gcaggtcgcg ggtgacgccg gtcggattgg tgatcagccc gacgcgtttg
    21481 ccggccagga gggaaaagtc gtcggcaatc agaacgtcga cgccgttttg aacggccgcg
    21541 tcttttgcgg ctgcaggtgc ggcggcggcc catgagggaa cgaatccgac gcacaggaga
    21601 acgtccagaa acaatgtcga gattggaaac actctccggt actgatgcat ggctgaacac
    21661 tccataagat ctctacaggc ctcaaccgac gacccggcga ccgtcatcac gacatccggc
    21721 cgttgaaccg atgaaccgtc tcgaccatgg ccaggaagtt gtcggcaggc acgtagttcg
    21781 tgacggagtt gccggaaccg caggcgtatc cccctccggg cgaacatcgt tcgaggatct
    21841 cgcctgtacg ccgacagacg acctcgcgcg gagcccgact caacagatca acatccaggc
    21901 cgcccaggac ggccactcga tcgccccacc gccccttgaa ttcctcgacg ggcacaatcg
    21961 cgtcctcgaa actgtgtttg ccgtcgatgc ccacgtactc gatcagatcg ggcatgaccg
    22021 actcgacgta tccgcaactg tggaggagga aaaaccggcc cgttcggtgg gccagttccg
    22081 cgaaacggcg gtgccacggc aggatgtact ctcgcaggtg atcgggactg atcaatgtgg
    22141 cggttttgaa acccatgtcg tcgcccagcc acagagcaaa cacctcgggc atggtgcaga
    22201 aacgctcgaa agcggccagc aacagcgatc cgacgcgatc gaccacctct ttcaccaaat
    22261 cgggctcctc atggagcatg atcatgaaat gctcgagtcc catgagggtg ccggcccatt
    22321 cgagcacgcc gcctgagaaa ccgatggcct ccatgccttc gggcagacaa gcgatggccg
    22381 cctctgcctg ctcgaaaccg aacgaatcgg ggctcggcca gggatatctt tcgatgtccc
    22441 ggcgggtctg gatcaggccc tgctgctcgt tgacccactg tctgccgacc accgcggcgg
    22501 gcgccccttc agacgcttgt ccgtaatcga ccgtgaacgg gatgttcact cgcaccctgt
    22561 agtagtcata cccgagccgc caccataatc gcaccctctg ttcggcccag gcccgcaatc
    22621 gagacttttc gccgtcggca ggcggcggca ggagagaacc ggcgttcaac tccgcaagca
    22681 cttcctccgc gatggccaac tcaaacagcg ggatccggtc gggacagcct cggcgcagaa
    22741 cggtcttcag tcgctccaca tcgggctggg gttcgggcaa atgagccagc ggatcatcca
    22801 ttaccaagtc gttcctcttt cgcgacgagc cccctcgacc acccggtccc agtcgaacgc
    22861 cggaccggga tcgatctttt ccttggtgac gtgcatgtga cccagcagtc ccgaccacgc
    22921 ggcaagttcc tgctcggaga agacctcagt tcgcacgcgg ccgtcattgc cgcgagggta
    22981 atcgagcctc agtcgcggga agatgcggtt cagcgccgca gtcagcttga tcagagcgtc
    23041 atactgctcg caggtgaagt cgtattgcat caggtcgcgg ccgttgatgt tgccggtgac
    23101 cacttccttt ctggccgctc gtcccatgaa ccagggcgtc cggacccgac gttctttcac
    23161 ccacttgggg ggtgacacgt acggccagcc aaacaagtcc ttggcatacc actcgtcgag
    23221 cgtcttcatg tcgccgtagg ctccgatatg agcgatctcg acaccgaggg agcggtcgtt
    23281 ggccgtgccg gcgtgccaag cccgctcctt gagatccagc gtctggtaga cggtcccgtc
    23341 aatatcgagc aagaaatgga cggacaggcc gcggaggtcg tgcaggacct tgaaacagcg
    23401 agccgatgtc ccgcatgcgt cgtagtgaat cacgaactga tcgaccaccc gtcgggcttc
    23461 atcgaggctc catccgcggt cctttatacg ctcctcgacg tccttgggca ggttccgccg
    23521 ggttccgtag cggttgggat ccagcggagc ggccggcgtg tgcggcatga tcagttcggg
    23581 ttcaaaatgg cgccggcagc ggtaagcatc gtatccgccc gggtccatcc agaggacaac
    23641 aggagtaccg gtgtgaaaga accggccggc ggcaacgatt tcgtcgccgc accgcatcag
    23701 cggatcgccg gggcgctggc cgtatctggt cggcgaacac ccgcacaacc ccgataggac
    23761 aacgatcgca aacgcggcaa acaatgctcg gaggtttcct gtcatgatag agcctcccgc
    23821 acagcgctcg tcactcagtc atcaaccggc tgtagatcga agctgaaatc ccagtcccac
    23881 tcatcataat agaggttgcg gccgtcgatt tcgacttcac cccgctgaaa atcggcgggc
    23941 tcgcttcgca agctgtgctt gggcggatcg agctgccagc catagtccag attcacgatc
    24001 atccggtagg aatccatatg gccgaagaag aagtcgcgta tcctcgggtc atccgacttc
    24061 ttgaggccat aggaagcgtc ggccgggagc cagccccacg gctccacgta gaactcggcc
    24121 cagtcgtgca tgttccattc gagcggttgc gtttcccagc cggactgcca tcgagcgggt
    24181 atgccgactg agcgacacat ggcgatgaac aacatggcgt ggatgccgca gtcgccccgc
    24241 caggtggcca gccctttacg cgtgcaactg gggatgatcg agtactcctc ctccgcgcag
    24301 tacttcatcc tggtgttgat gtagtagaag atcttccgag cctgtgccag gggattggtc
    24361 tcgtcgccga cggcctgttt cacggcctcg cgcagctcgg gtgtaaacac gacgtgcggg
    24421 ggccgttctt ccagacaagc cttaccccag tcgtccggca ggggtcgagc ggcggcatcg
    24481 tccagcttcg ggcagaacgc atagcagaca tactcgaact cttcctcgaa gaccaccggc
    24541 tgggccggat cgctgatctt ctgttccagg tacacggtcc gctgggcagc accggtgagg
    24601 atctgattac cctcggcatt gggtgcaagc ttgcactccg tcggcgcggt gcggatcagc
    24661 cgcacctgct tctgctggcg gtattcctgg gggaacggca gccagcatcg caccagggag
    24721 ccggacctgg cgccagggcg attgggggcg acggtgagct tgtaccggat gcgttgccgg
    24781 atgggtagca cctcggtctt gcctgatgcg tcggccgcgg ccagcgcctt gcggatgtgc
    24841 tcgatcatct gctgctcggc ctgtgccttc ttgtccggtg caggagcggc ctgcgttgcg
    24901 gcatgcgcat cacgacgctg cctggcttct tcgcagaaac ggaagatgtt cgacggttcg
    24961 cggatgaaat agcacggccg gccgtctaaa acgcggtatt gaacctcgcc ggccgcccgc
    25021 caccgctcga cgtcggccgc ggttacatcg gggatgggcg agcggagccg ctcgacgagc
    25081 gccggcaatt ccaggcggta gtcgcgtcga atccggcgga tgatttccag ggcgtcggcg
    25141 gcggcttggc tgcgacgtgg atcatcgatc ttctgttcgg cctgcaagag gttctcggcc
    25201 tcacgcagac gaccgtcggc gaccaactgc cgcacgcgaa ccatgatggg ctcatcagag
    25261 gtgaacaact gtgcggcccc cgacagagga ccggcatccg tttgtccggc ggcggccagt
    25321 gcactcaata gcatcgacat ccagatcatt gttgttcctc atctacgacc gacgtttccc
    25381 gtcaaccatt ccactcagag tacatcccaa gacagtggct tcatcctcag acaatgagca
    25441 catcctaaga gagtgtgggt tccgcttcca gcccgccgct cagtccgaaa tccgttccgg
    25501 ggcccgggcc cggaaaggat tccgggccga gcggctggtc ccagattcct atctgggacc
    25561 cttctggtga cgattcccat cgtcattcat atgtgaatgc ggaagggatt ccgcgaggag
    25621 tgcggcccgg aaaggattcc gggccgagcg gattcatttg gaggcgcatg cccac
//
LOCUS       DAOTPW010000053        25197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2057_length_25197_cov_5.626879, whole genome shotgun sequence.
ACCESSION   DAOTPW010000053 DAOTPW010000000
VERSION     DAOTPW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 25197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25197
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_25197_cov_5.626879"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            559..1965
                     /locus_tag="P5316_06775"
     CDS             559..1965
                     /locus_tag="P5316_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HRR84697.1"
                     /translation="MKAGQIDAGQEVNHRDTGSDTSGISRSLDTARASVRVECRPIIP
                     LLRYQQADIESPARFSWCCWSRQTGKSFTKSLKRLLRGLARRRNQILLSAGERQSAEL
                     MLKVRQHCRALNIACSFHGDRFFEGTRFRQLTIELSNGVRIIGLPANPMTARGFTGDV
                     LLDEFAMHRDDRAIWASIFPTILRGGGELDVASTPKGRDNLFAELRTNERFARSVVTL
                     HDAVAAGLEVDPEEIRCSMSDDELFRQEFLCEFLDESSVLLSHELIAGVEDASLDKAL
                     DETRLQRCKRPLYVGVDIGRCRDLTVIWVLEAEGDILVTRGVRESMGEPFREQYQALR
                     RLLSLRQVTRCCIDAGGIGMALAEAAVDEFGGSRVEPVTFTAAVKDELANRLRLRVEE
                     QKIRIPADEAIRNDWHSVRRSVTTAGPARYDAARSGGGHADRFWAACLAIRAAGQPAG
                     PIEYLHGPGLRFGREGTW"
     gene            2184..3473
                     /locus_tag="P5316_06780"
     CDS             2184..3473
                     /locus_tag="P5316_06780"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HRR84698.1"
                     /translation="MGLKRLIAHAFGRATKAMRPSVGQMVQPMAADRWRDYPADGLTP
                     ARLVQILREADDGAIDRAMALYQQMEEKDAHLYAVARTRRQALTGLSWQVISAADLHD
                     VPDRAAADEAADYCGEVLSGIERFDDALEHLSLALGRNIAVAENVWDFADGELRLVDI
                     VPVAFERLTFDEAGKLRVLTEEAPYEGIELPPNKFIVHVPHAASGHPMRGGLLRASAL
                     AYLGKHLAMKDWLVYAELFGMPVRVARYEPSATPEEKRELLKMLESLRADAAGIFSKA
                     VELQLLEAGQGKSPPPYEHICEFFNRELSKAWLGGTLTVETTGAGLERSSGGPSVHNE
                     VRLDLRQDDIASEGRTIRRDVLGPVARLRFGDRVPVPYFRRQLELPRDRRELSEVLDR
                     AVNELRMRVPARWAHDVLGIPMASEEETVLRGTLGSG"
     gene            3641..6184
                     /locus_tag="P5316_06785"
     CDS             3641..6184
                     /locus_tag="P5316_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRR84699.1"
                     /translation="MPNGDCRTWRVPLFMLTIAVAGSAANAAITLIGVQYKPDRALPE
                     YECFWHPDQLPEPCGPSAPMGASVHVFLLNSGPSSVTVQDVTLAGISLELALYEEEQV
                     VKRHPVSIYFALAHGAITQQQFNTLISAGEPVWYKADPKTIPAGGTAQIVVRLRQVPT
                     TPTVGIAVIHTGGSTVATVPVDADQPHAAGVSFSSDLTKVYLYWRRSNGTAPTTILFD
                     GSDVTASTTTVNDPSVETAASVLRSAQPLSPASFHVYQGIYADGRVASAGVRTWANPF
                     IYGTWGAKDAPDGDYAAARAWIDDATNHCVNSLVVQGGSEALKSYLKTAEGRQYAADH
                     GYGFVIDEIGKWSCQNPQLWFIRDEPDAADSRVTGLPGDRMVGSLADMAVERGEELRA
                     ADPTAPTTLNIDSTYTPFNWYNYGQVPDVLMTDPYYQNRLREALWFYPTRIPLYSKAA
                     YIYAVSRVARSSCEPNPLHVVLYSCEYVDTTTGQRFPFAPPACKRIEVYYALAAGATG
                     LSYWWYLPGKPSNGLGAGTPAALALWREIGLLGAEIRTAAPLLVLGCPAELSLQTSSG
                     LWVRSLVAGTDALLLFVVNDQYYNDEQGCHYTPVAGASLTVSLPAWLQSPEAFEIVAS
                     GIRDTTTQVAGNQLQVNLGTVDVTRMIVVTSNPLLRDSIEQRYAQEVHPKLCAFAPDA
                     CLPVITQQPIGQVATAGSTVSFTVAASGVGTLSYRWQKNGSDLGDGGHYSGCTTTTLT
                     ISNVDNADAAGYRCVVSTSNGIAVSRQVQLSIVPAQIPGDFDGDGDVDQEDYGMFQRC
                     LSGVTIPQEDPACAAARLDADGDVDRDDAALFLRCISGRDAAADADCAD"
     gene            7248..8360
                     /locus_tag="P5316_06790"
     CDS             7248..8360
                     /locus_tag="P5316_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRR84700.1"
                     /translation="MLKRVRVTKYQRGLLFKDGDFRGLLNPGVYWYSPLRRTRIEVAG
                     ILETRLAHPLLEILLADERLRAAVEVINLTDAQRALVWLDERLTYVLGPGRYAFWKEP
                     YRLRIEIFDIAQVRLHKPELEALAGRPDIALWVNVIQVGPEEEALVFQNGTLIDRLRA
                     GTYLYWKSTSFACRKVSLKELVADIAGQEIMTQDKVTLRLNLSVGYQVVDPVKAVSTV
                     DDYAQAFYREAQLALRAAVGARTLDALLTDKQAVGQEIIEALSKRAAEMGLAVRNVGI
                     RDIILPGDMKQLLNEVLAAEKRAQANLIKRREETAAARNQANTARLLAENPILARLKE
                     LELLQEILADSKPTFVFGNTNLLDQLRSLTGKPVGS"
     gene            8471..8653
                     /locus_tag="P5316_06795"
     CDS             8471..8653
                     /locus_tag="P5316_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84701.1"
                     /translation="MIRDPIVEDIREARRKTEADCEGDWDKLVEHYRQVETHAGKRVI
                     QGSPRRPATTRTNPRQ"
     gene            complement(8697..10613)
                     /gene="asnB"
                     /locus_tag="P5316_06800"
     CDS             complement(8697..10613)
                     /gene="asnB"
                     /locus_tag="P5316_06800"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRR84702.1"
                     /translation="MCGIAGIIDFAGRLMDPDLLRNLCGCLAHRGPDDTGIWAAQTPG
                     FSVGLAHTRLAVIDPTPDGHQPMTDPSHRRAIVYNGELYNYRELREQLPGPFETACDT
                     EVALAACMHWGPEALRRFDAMWAMAYVDLERRSGHLSRDPMGIKPLYWTFHNHRLVFA
                     SELSTLCRIPEVRREIDPQAVALYLTLGWIPHPRTIFAGVQKLSPGHCLFFDARGPMN
                     PEPFFRLNPLPAPPPAYADAVCELRRRVEEAVLAQRIADVPLGAFLSGGLDSSIVTAC
                     LARAGPTPVETFSIGYADHPRYDETEYAELVARHLGTRHHTFRLAFKDVLDAVEPVLN
                     HLGEPFADSSLLPAALVSRYTRRHVTVALSGDGGDELFAGYWRYAGHHYLARYRRLPR
                     VLRRGVIEPMLKLAPQARSNRLLDRLRQARKLLRGDLADPLGTHLAWARIIDQRQATE
                     LLGPERAAGAGEYLVNLYRDAPARLRADAAELTPLDRILLADLAIGLPADMLHKVDTA
                     SMLHSLEVRVPLLSVDVVNFAASLPIEYKLQGSIGKRILRDAFRDVLPEPILNRKKMG
                     FEVPVGEFLRNELAPMYKDLVTKKALQDFGVRHEAAQRLYEAHCRRRADHTEILWALL
                     VLCHWKSRKNQDGR"
     gene            complement(10754..11200)
                     /locus_tag="P5316_06805"
     CDS             complement(10754..11200)
                     /locus_tag="P5316_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84703.1"
                     /translation="MGLLVPLLGMGIGAGLPLLSSGCAGRYDVNEVLRYLVERRGLDT
                     GENTVTVRLSNQTAGYDERVTLRIDGLTSVFECPADEMVCDYILVEIPDLIEVIEEQR
                     YDEDGGFAGGRILEGQPGFTLDKTQYRPGSIIVLQMGVESARVRVL"
     gene            11608..12654
                     /locus_tag="P5316_06810"
     CDS             11608..12654
                     /locus_tag="P5316_06810"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRR84704.1"
                     /translation="MYRPIFRAACNRRMAGLGAAMLCLPCILAGAAMAADLPQVTIDS
                     TAVTLHLGGRPVGRYRYAEVPFKPYLQELRTPSGINILRDAPFDHLHHHSLMFAVAVE
                     GVNFWEEKDGFGKQEHLAIRDVFAGHAGGLPCASFKEEVAWRAGGKGLLHETRAVRVL
                     ADPAGKASVVSWTAEFRELNPDGRTAGAGQPAVTLGGERYYGLGMRFITSMDGTGQFF
                     NADGANGVDGTNDKRSRWCAYTAEADGKPVTVAMFDHPTNARHPANWFTMLEPFSYLC
                     ATLGLQHEKLALGRAASQPADQQAGAGPFRLTYGVAVWDGKVQPDEIERVYALWLKTT
                     PAQETGESTNVKAK"
     gene            12801..14069
                     /locus_tag="P5316_06815"
     CDS             12801..14069
                     /locus_tag="P5316_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR84705.1"
                     /translation="MSQTKPVDYAAVSTKTPPGLGMGVRLSAMMFLQYAIWGIWAPIL
                     GLHLGALPGFQTPEGAADFGRIGLIYMTMPIASIIAPFIGGQIADRYFSAERFLAFSQ
                     LLGGVVLLVVSQLTTFAGVFIGMLVYNLLYAPTIALTNSITFQHWPNQQFSKIRVAGS
                     IGWIVIGWIFTVWMAKLGHAFRTPAMGDCLYGAAILSIAYAGYALFLPHTPPSKQAGN
                     PLAFLDAVKMLRNPAFAVMAVVSFFVAIELQLYFIWAQSFLKSGVGIPEGWVSTVLTA
                     GQVCEMVMMVLLPLALLKWGFRATMAIGIAAWCLRDLVFAVGQPTSLVVGAVGLHGVG
                     YAFFFTTIFMFADAIAPKDIKSSAQGFLASVTIGCGMLIGSLLAGPISTACDNQWSKI
                     FLVPAILCAVCCVAFLIGFREKRDQAAVPA"
     gene            complement(14141..15466)
                     /gene="hemL"
                     /locus_tag="P5316_06820"
     CDS             complement(14141..15466)
                     /gene="hemL"
                     /locus_tag="P5316_06820"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRR84706.1"
                     /translation="MAKQKSETDETQKNKKSQAAFTAALRVLAGGVNSPVRAFGAIGG
                     VPRFIAKAKGATLTDLDGNDYIDYVCSWGPLILGHTDERVAAAASKALDKGWSYGAPT
                     EAETRLAERIVEDFPSIERIRFVNSGTEAVMSAVRLARGFTGRDLVVKCEGCYHGHVD
                     YLLVKAGSGLATFGTPSSAGVPTAMTANTLVVPYNDLGAAREVFERHGSRIAAFLVEP
                     VAGNMGCVPPVDGYLAGLRQLCDEAGALLIFDEIVTGYRVSVGGAQEAFGVRPDLTCL
                     GKIIGGGMPVGAYGGRADVMEKLSPVGPVYQAGTLSGNPLAMAAGLATLEALHEPGTY
                     ERLDTLATRLEDGLKAAAATAGVPAFVTRVASMCTLFFQVGPVTDYASAARSDTRAYA
                     QFVHAMLERGVYLPPSQFETFFVSAAHTEEQIDQTLAAAAEAFQVLKPE"
     gene            15707..17029
                     /gene="dnaA"
                     /locus_tag="P5316_06825"
     CDS             15707..17029
                     /gene="dnaA"
                     /locus_tag="P5316_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRR84707.1"
                     /translation="MTSISERAWNDILASVKASNPELARPWFAALQPLDMRHGVIRVN
                     TTTPEQHHYLTHDCQQAFNSAAQSITGRLVTVHFVSPPTPAPRIEPLAFERETESIVL
                     NGNYTFDNFVTGPCNRLAHAACVAVAENPGSVYNPLFLHGAVGLGKTHLLQAVCHHIR
                     SQQPESKILYLSCETFTNHFVEAIERGALHQFRYRYRHVDALVIDDVQFLAARERSRE
                     EFFHTFNTLFQLQKQIILSADESPRDIPSLEDRLVSRFNSGLVTRLEAPCLETRMAIV
                     SHKAKLRCADVPEDVVHFIAASVDSSIRELEGALTRVLAVAQQYGGTVDLGVAREALA
                     QSPAAGNRRITISDILQMVTERYNVRLSDLQGKRRHRSLAFPRQICMYLARELTPLSL
                     EEIGGHFGGRDHTTVLHAIRTISEQRSVDQELEANIRQITALLSKQKA"
     gene            17533..18633
                     /gene="dnaN"
                     /locus_tag="P5316_06830"
     CDS             17533..18633
                     /gene="dnaN"
                     /locus_tag="P5316_06830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRR84708.1"
                     /translation="MQVTVNRNALVEVLNMAAPVAASRTPRELLKCVRLTTVDGSLLV
                     GATDLEVALRGEVRQVEVKVPGAALVPADKLMQIARECPDETLNIESEELVCRIRGAD
                     SHFEIYGQDPKDFPPVPDLEGTPDVEVEGSVLTSLIERTLFAVAKENTRYAISGVLWE
                     TKGKRLSLVATDGRRLAEANGPVARSTGGDQRMIVPAKTMQVLQRVLPHVPEDTPVGV
                     RFSSNQVVVRAGLFVVSSPLVEGQFPKYEDVIPKDSDKKITLSTPDFHSAVRRAALLA
                     SDESKGIRLAFDAGMLVLSSRTPEQGEATVSMRTDYQGQPLQIGFNPAYLAEALRVVS
                     SPTVQMELKEGNRPGILRAGAEFLYVIMPVSL"
     gene            18659..19096
                     /locus_tag="P5316_06835"
     CDS             18659..19096
                     /locus_tag="P5316_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84709.1"
                     /translation="MNDEQLRWVQYNHGETGARARPPVETERTRQREEREAARRGAVT
                     QIGKLVRAMVEKRSGLTALQQKLLAILDEEGGIDLAAQVTGLGLYQGILRIETADPVT
                     LCDLRMRWQRRLLEAVQSRLPEAGIRAVRFSLAGRSSQSDSQE"
     gene            19111..21708
                     /gene="gyrB"
                     /locus_tag="P5316_06840"
     CDS             19111..21708
                     /gene="gyrB"
                     /locus_tag="P5316_06840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRR84710.1"
                     /translation="MTEAASEAQGVPTGEPAEQSATPTESRYDESSIRVMEGLEAVRK
                     RPAMYIGDTGITGLHHLIYEAVDNAIDEAMAGYCTEIAVTLGADGSCAVRDNGRGIPV
                     GPMTHPDPKINGKPAVEVCMTVLHAGGKFDRRSYKVSGGLHGVGISVVNALSEWLRVQ
                     VRQGGNVYEMGFERGVTTSPLKIIGHAVDTGTVVKFKPDAQIFPDHDFRYETLRSRLR
                     ELAYLNEGVRITLTDERTGKSEVYHFADGLREFVAHLNEGKEPIHKDIILMRAQDQEQ
                     RLVVEIAMQWNDGYNENVSCFANNIRNIDGGTHLSGFRAALTRTLNYYAKKENLVKGD
                     LVTTGEDFREGLTAIVAVKVPEPQFEAQTKVRLMNPEVETFVTQVVNSQLSTFLEENP
                     ADARRIVLKGIQAAQAREAARKARELTRKSALSSGGLPSKLWDCRVKDADTAELFIVE
                     GQSAGGSAKTGRDSEFQAILPLKGKILNVEKARIDKMLSHEEIKTLILALGCGIGEDE
                     FDISKRRYGKVVLMCDADVDGSHIRTLLLTFLFRHMRPLIEGGYVYVAQPPLFLLKKG
                     KKSEYVLNDAVLSRKLKEWGTEDTTLVVRAADGTEREIAGPELAELYTLLERIDAQRR
                     ILERRGIDQRELLTRHVDEQGRLPAYRAVVYRPGDPEPATYFGYSDEEFQAFCEQERQ
                     RLGEVEVVEAGAGMIPPKNNGNMAHRIIRSDLSECAVLEEAMRKLGAFGLSMDDYYAE
                     RVEHVDGTVDPARFVLKHGGQVVREVDGLRHLVEAIRQVGSTGVEVKRFKGLGEMNGE
                     ELWETTMDPERRSMLKVVISDEPDDPEQLALDAREADRMFSILMGDNVELRRQYIEAN
                     AAHVKNLDI"
     gene            21831..24500
                     /locus_tag="P5316_06845"
     CDS             21831..24500
                     /locus_tag="P5316_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRR84711.1"
                     /translation="MGASPGRWAYREGRLDNGLRVLTLEDHRSPLVCVQVWFHVGSKD
                     EDPQRQGFAHMFEHMMFRGTDRIGPQDHFRYLNRFGARVNGYTSFDETVYWEVLPASQ
                     LDLALWLEAERMGRLKINEEYFAAERNVVKEERRMRYLNRPYGRLYETLFDAAFKVHP
                     YRWTPIGNIPHLNAATAEELQAFFEKFYVPNNATLVVVGDVQHDDVMAKATRFFGTIS
                     RRPDPVRVGVVEPPVTEPRRVEIADRAPSPRVVIAYHTPGARDPDSLALEVLSKILSS
                     GQSSRLFRQLVQERQIAVSVFGSAYTLEQAGLFALSAILKPEVSIEEGEQALLEEIRR
                     LLEQGIEPAELEKARNQTLAEYVQRGETVQGRADLLGYAAVILGDPERVNTDLERTRA
                     LSAEQVMAVARRVFGEQGRVTVVIRPDPNPPADDEEDTKAPEEKPIPDLPPPPEMPQG
                     DSPRPVKLASPVMRQLENGLRVAVFSDHSVPAVTVALNVLVGAASDPPEAAGLAYVTA
                     NTLRRGTARHSGDELAEIIDSHGMTLSESVDHYESGIRMWTLSEHVQLACRTLAEIVR
                     EPSFPQREVKNFVERAVARERIEEENPATIAGRTLDEAIFGDNPLARPEGGTSTSLKG
                     ITRDQVTAFHRQCYAPNVATLVFAGDVTAEQATAMAEECFGGWGGRATFGDPPAPPPR
                     GDLKVLLVDRPSASQSEIRIGQVVPVSRRDGDYAAVRLLSMVFGESFSARIGRVLRIE
                     KGLTYGARGSFEVEGNAAAFRVSTFTRTEKTADAVQAALAEVDKLLTGDVSREELALA
                     RDQLIGGFQVGLETPWQTASRWWDLKVWGLPEGWYEDYQAAIVRVNDPAVLRQAAART
                     IEPRKLTIVVVGNGEGVREGLSRIAPVESAPAP"
     gene            24563..24961
                     /locus_tag="P5316_06850"
     CDS             24563..24961
                     /locus_tag="P5316_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84712.1"
                     /translation="MSEGPSGRRGAGGAVFGALLGVNFAALVLLGGVLLVLLPKMAAV
                     YAAFGTPLPAVTGILLQVSQARAAVLMGLGLLLAGQVTLARVAGRGRHVVLLVGVLVE
                     IVLVVAVAFAVFVPYVELINSISGSNGAGG"
BASE COUNT         4838 a         7555 c         8257 g         4547 t
ORIGIN      
        1 ccgagaagca cccagcggcg cggctcccag ggaacctcct atactgctat ccggacctat
       61 actcccatcc ggatcgtaga gggatacgct gagaacaaga tccgaccggg ccaaaaccgg
      121 caggggtttc ccgccctccg ccggcggggc tcctgagact ggccgacagg tcacgccgtt
      181 caccgccgta agaaacctcg gtgagggcct tccagcatcg ctacggctcg cggtcgcttc
      241 ttcccgcccg cgccaatcgg cgcgcttcta cccccctgac cttaccgcgt caagcggttc
      301 aaagcggatc cgtcagcctg aggggtacga atcgagccgg ccgcagcgag tcgaacatct
      361 cgctcatgca ataagtcctt tactgaaaac ggcctatgcg catccatggg ctgagtggcg
      421 acctcgaaaa cggccgggca tacccggctg ccaagagcga gcgcggctta acccgttccg
      481 ctcagaaggc cggttccgga gcagcccagc gcgttatggc cacgtgtgga caacacgcgc
      541 cggaggtgaa ggctgggcat gaaggcaggt cagatcgacg cgggtcaaga ggtaaaccat
      601 cgggacaccg ggagcgatac ctcgggtatc agccgctctt tggatactgc cagagcatcc
      661 gtccgggtgg agtgtcggcc catcatccct ctgctcaggt atcagcaggc cgacatcgaa
      721 tcaccggcgc ggttcagttg gtgctgctgg tctcgccaga caggaaagag cttcaccaag
      781 tcgctcaagc ggcttctgcg agggctggcc cgccggcgaa accagattct cctctcggcc
      841 ggcgagcggc aaagcgccga gttgatgctc aaggtgcggc agcattgccg ggccctgaac
      901 atcgcgtgca gtttccacgg tgaccgcttc ttcgagggaa cccgtttccg tcaactgacg
      961 atcgagttgt ccaacggcgt gcggattatc ggacttccgg ccaatcccat gaccgcccga
     1021 ggtttcaccg gggacgtctt gctggacgag tttgccatgc accgcgacga ccgggcgatc
     1081 tgggcgtcga tctttccgac cattctacga ggcgggggcg agttggacgt ggcctcgacg
     1141 ccgaagggtc gcgacaacct cttcgccgaa ctgcggacca acgagcggtt cgctcggtcg
     1201 gttgtgaccc tgcacgacgc cgtggccgcg ggactggagg tcgatccgga ggagatccgc
     1261 tgctcgatgt cggacgacga gctctttcgg caggagtttc tctgcgagtt tctggacgag
     1321 tcatcagtcc ttctgagcca tgagctgatc gcgggcgtcg aggatgcctc gttggacaag
     1381 gcgctggatg agacgcggct gcaacgctgc aagagaccgc tctacgtcgg cgtggacatc
     1441 ggccgatgca gggacctgac ggtgatatgg gtgctggagg cggaaggtga catcctggtc
     1501 acgcggggtg tgcgggagtc gatgggcgaa ccattccgcg agcagtatca ggcattgcgg
     1561 agactgctgt cgctgcgaca ggtgacccgt tgctgcatcg acgcgggcgg catcggcatg
     1621 gcactggcgg aggcggcagt ggacgagttc ggtggatcgc gagttgagcc cgtcaccttc
     1681 accgccgcgg tcaaggatga actagccaac cgcctgcggt tgcgggtcga agaacagaag
     1741 atccggattc cggccgacga ggcgattcgc aacgactggc actcggttcg acggagcgtg
     1801 acgacggccg ggccggcgcg atacgacgcc gcccgctccg gcggcggtca tgccgaccgc
     1861 ttctgggcag catgcctggc cattcgcgcg gccggacagc cggccggccc gatcgagtac
     1921 ctgcacggac cgggtctgcg attcggtaga gagggcacct ggtagtccgg caacagcttg
     1981 ctccttgcag acggcggacg acagagagca aaacggcacg aggaccggga atgagatctc
     2041 acatttgaga tttgagattc cagatcggcg atcggaggtc ccggattgga gatctgaggt
     2101 ttcaaatgtg aaatcagaga ttgcggattg gcagttggaa tcgaagacca tcgatcacgt
     2161 aatcgcaaat ggaggaccaa cagatgggat tgaagcgctt gattgcacac gcgttcggac
     2221 gagcgaccaa ggcgatgcgg ccgtccgtgg ggcaaatggt acaacccatg gcggcggacc
     2281 gttggcgcga ttacccggcc gacgggctga cgccggcgcg cttggtgcag attctccgcg
     2341 aagccgacga cggagctatc gaccgggcca tggctctgta ccagcagatg gaggagaaag
     2401 acgctcatct ttacgccgtg gcccgcacac ggcggcaggc gctgaccggg ctgtcatggc
     2461 aggtgatctc ggcggccgac ctgcatgacg tcccggatcg agcggcagcc gacgaggcgg
     2521 ccgactattg cggcgaggtg ctgtcgggaa tcgagcggtt cgacgacgcg ctcgaacact
     2581 tgtccctggc tctgggccgc aacattgcgg tggccgagaa cgtctgggac tttgctgatg
     2641 gcgagttgcg cctggtggac atcgtgccgg tggcctttga gcggctgacc ttcgacgaag
     2701 ccgggaagct tcgcgtgctc accgaggagg caccgtacga gggaatcgaa ctgccgccga
     2761 acaagttcat cgttcacgtg ccgcacgccg ccagtggaca cccgatgcgc ggcggcctgc
     2821 tgcgagccag cgccctcgcc tacctgggca aacacctggc gatgaaggac tggctggtct
     2881 atgcggagct gttcgggatg ccggtgcggg tcgcgcgata tgagccgtcg gcgacgccgg
     2941 aagagaagcg cgagctgctg aaaatgctcg aatcgctgcg cgccgacgcg gccggcatct
     3001 tcagcaaggc ggtggagctg caactgctgg aggcgggcca aggcaagtcc ccgccgccct
     3061 acgagcacat ctgcgagttc ttcaaccgcg agctgagcaa agcgtggctg ggcggcacgc
     3121 tgacggtcga gaccaccggt gcggggctgg agcgatcctc gggcggaccg agcgttcaca
     3181 acgaagtgcg gctcgatctg cggcaggatg acatcgcctc tgaaggccgc acaatccgtc
     3241 gcgacgttct cgggccggtc gcacggctgc gattcggcga tcgggttccg gtgccctact
     3301 tccggcggca actggaattg ccccgcgatc ggcgtgaact cagcgaagtc ctcgaccggg
     3361 cggtcaacga actgcgtatg cgcgtgcccg cccgctgggc tcatgacgtg ctgggaatcc
     3421 cgatggcctc cgaagaagaa acggtgctcc gcggaaccct tggttcgggc tgagaagaaa
     3481 tctctcgaag aacgtcggcc cacacaaagg ggcagaacaa cgatcgagaa caccttctct
     3541 ccttccaatg ccgcagcggc acgcatgtcg tggccgggac gcgttgcatt tgctaagatg
     3601 gttcaggacc gcgaccggcc cggcagagag gaggaaggtg atgcccaacg gtgattgccg
     3661 cacttggcgc gttccgctgt ttatgctaac catcgccgtt gccggctcgg cggcaaacgc
     3721 ggcaatcact ctgatcggcg tccagtacaa gccggaccgg gcattacccg aatacgaatg
     3781 cttctggcat cccgaccagt tgcccgagcc gtgcggtccc agcgcgccga tgggcgcgtc
     3841 cgtccacgtg ttcctgctga acagcggccc ttcctcggtg acggtccaag acgtcacgct
     3901 ggccggcatc agcctcgaac tggccttgta cgaggaggag caggtggtca agcgtcaccc
     3961 ggtcagcatc tatttcgcgc tggcccacgg ggccattacc cagcagcagt tcaacaccct
     4021 gatcagcgcg ggcgaaccgg tctggtacaa agccgatccg aagacgatcc cggccggagg
     4081 cacggcgcag atcgtggtgc gactgcggca ggtacccacc actccgacgg tcggtattgc
     4141 ggtcatccac accggcggtt cgaccgtcgc aaccgtaccg gtcgatgccg accaacccca
     4201 cgcggccggg gtgagcttct cgtcagactt gaccaaggtg tatctgtact ggcggcgcag
     4261 taacggtact gctcccacaa ccattctgtt cgatggcagc gacgtcaccg cgagcacgac
     4321 tacggtcaat gatccgagtg tcgagactgc cgcatccgtc ctgcgatcgg cccaaccgct
     4381 ttcgcccgcc tcgttccatg tgtaccaggg catctacgcc gacggccgcg ttgcctccgc
     4441 cggtgttcgc acgtgggcca atcctttcat ttacgggacc tggggcgcca aagacgctcc
     4501 cgatggcgac tacgccgccg ctcgtgcgtg gattgacgac gcgaccaatc actgcgtcaa
     4561 ctcgctcgtg gtacaaggcg ggtcggaggc cctgaagagc tatctgaaaa cggccgaggg
     4621 caggcaatac gccgccgacc acggctatgg atttgtcatt gatgagatcg gcaaatggtc
     4681 ttgtcagaat ccgcagttgt ggttcatacg ggatgagccc gacgccgccg attcgcgggt
     4741 caccggcctg cctggggaca gaatggtcgg ttccctcgcc gatatggccg tcgagcgcgg
     4801 cgaggaactc cgggccgccg acccaacggc gccgaccacg ctcaacatcg acagcaccta
     4861 cacccctttc aactggtaca actacgggca ggtaccggat gtgctgatga ccgatccgta
     4921 ttaccagaac cgcctgcgcg aagcgctgtg gttctaccca acaagaatcc cgctttacag
     4981 caaggccgcc tacatctatg cggtctcgcg cgttgcccgg tcaagctgcg agcccaatcc
     5041 gctgcatgtc gtgctgtact cgtgcgagta cgtggacacc acgacaggcc aacgcttccc
     5101 gtttgcgccg cccgcgtgca agcgcattga ggtctactat gccctcgctg ccggtgccac
     5161 ggggctgtcc tactggtggt acctcccggg caaaccgtcg aacggcctcg gggccggcac
     5221 cccggccgcg ctggcacttt ggcgggaaat cgggcttctc ggcgccgaaa tccgaaccgc
     5281 ggcgccactg ctggtcctgg gctgcccggc cgagctgagc ctgcaaacca gttcgggact
     5341 gtgggtcagg tcgctggtcg ccggcaccga cgcccttctg ctgtttgtgg tcaacgacca
     5401 gtactacaac gacgaacagg gctgccacta caccccggtg gccggcgcga gtctgacagt
     5461 gagcctgccg gcgtggctgc agtctcctga ggcgttcgag atcgtggcaa gcggcatccg
     5521 ggataccaca acgcaggtcg caggcaacca gttgcaggtg aatctcggca cggtggacgt
     5581 gacgcggatg atcgtggtca cctccaaccc gctgctgcga gacagcattg agcaacgcta
     5641 cgcccaggag gttcacccca agctctgtgc cttcgcgccc gacgcgtgtc tgccggtcat
     5701 cacgcagcag ccgatagggc aagtcgcgac ggccggttcg accgtctctt ttaccgtggc
     5761 ggcctcgggc gtgggcacac tcagctaccg atggcagaag aacggctcgg acctcggcga
     5821 cggcgggcac tattcgggct gcaccacgac aacactgacg atctcgaacg tggacaacgc
     5881 cgatgcggcc ggctaccggt gcgtggtcag cacctccaac ggcatcgccg tttcccgcca
     5941 ggttcaactg tcgattgtcc ccgctcagat cccgggtgac tttgatggcg acggcgacgt
     6001 ggatcaggaa gactacggta tgttccagcg atgtctgagc ggtgtgacga ttccgcagga
     6061 ggacccggcg tgtgccgccg ccaggctcga cgcggatggc gacgtggacc gggatgatgc
     6121 cgccctgttc ctccgctgca tcagcgggcg cgacgcggca gcggacgccg actgcgcgga
     6181 ctgacggacg agatccgcct gtccttgggg cgatctcaag aaaacttgca catttccgca
     6241 gttttcgctt gcattttgaa aaagcgcgtg tatatatatg tccgcacagg agaatgcgat
     6301 gatgtattgc gcgatatcaa tgaatgtgaa agagggccgt tcctcaaagg agggctccgg
     6361 gtaggtttgt tcgatcaggt cgatcgtgca gattcacccc gaagctttgc ggcctcgggg
     6421 tttttgtttt cctgtcccgt gggcgcttgg ctcgatgacg gccgatgagg ccgagattcc
     6481 tgaagggaac agtctgaaga ggagatgaca acatccgcct cgggcggcac ggcgatggtg
     6541 cgcgcccggc gaactccggt ccccgtatca gcacgaacga gagggcttcg tgccggggaa
     6601 agggtgcgcc cgcgtggcag agtcaacgat ggttgcgctg gaggtgtcgg ccggcggcgc
     6661 acgcggccac cgaggaaggc gttgcaggcg tgtgttccgt gaacggcgtc gaccgcggta
     6721 cctgcggtgc aagctgccaa tagctcattt ggtagagcaa cgggccttta acccgtaggt
     6781 agcaggttcg attcctgctt ggaccaccag aaggtcgagt ctggaggtga gattcgaggt
     6841 gacgtcggtc gatggtcgcg gcggttgcgc ggcgggttat cccaaagcca acgcccactg
     6901 tcgacaccgt gtcgaaagcc ctgcaatccg gcggataccg ccgggccaat cgtcgtcgaa
     6961 ggcggcatcg agcttgtgtg tgcagcgaga caccgcatcg aggcgacggg aggcccgact
     7021 caagccgggt tccgccgtag ccacgggacg aaaccgcgaa tccacctccg gctcggacaa
     7081 tggccgcggt cgtgaggatg agagattagg aacctatgcc ggttgggtgc cacgggcggc
     7141 tcgtgtgtcc gtgccgccca tgcactggcg ggcaagcccc tcgacggcag ctcaggatga
     7201 aggccgccag tggcagccga gcgcattgtg aagggaggta tgcgatcatg ttgaagcgag
     7261 tgcgagtcac caagtaccag cggggtctgc tgttcaagga cggcgacttc cgcgggctgc
     7321 tcaatccggg cgtctactgg tactcgccgc tgcgccggac gcggatcgaa gtggccggca
     7381 tcctggaaac gcggctggcg cacccgctac tggagatcct gctggcggat gagcgactgc
     7441 gagcagcggt tgaggtgatc aacctgacgg acgcgcagcg ggcgctggtc tggctggatg
     7501 agcgcctgac ctacgttttg gggccggggc ggtacgcctt ctggaaggaa ccgtaccggc
     7561 tgcggatcga gatcttcgac atcgcccagg ttcggctgca caagcccgag ttggaggcct
     7621 tggccggccg gccggacatc gccctgtggg tcaacgtgat ccaggtgggt ccggaggaag
     7681 aggctttggt tttccagaac gggacgctga tcgaccggct gcgtgccgga acgtacctgt
     7741 actggaagag cacgagcttc gcatgcagga aggtgagtct caaggagctg gtcgccgaca
     7801 tcgcgggtca ggagatcatg acccaggaca aggtgacgct gcggctgaac ctgagcgtgg
     7861 gttaccaggt ggttgacccg gtcaaggcgg tgagcacggt tgacgactat gctcaggcct
     7921 tctaccggga ggctcaactg gccctgcggg cggcggtggg cgctcggacg ctcgatgccc
     7981 tgctgaccga caagcaggcg gttggccagg aaatcatcga ggcgttgagc aagcgggcgg
     8041 ccgagatggg tctggccgtc cgcaacgtcg gtatccggga catcatcctg cccggcgaca
     8101 tgaagcagtt gctcaacgag gttctggccg cagaaaagcg ggctcaagcc aacctgatca
     8161 agcggcgtga ggagacggcc gcggctcgga accaggcgaa caccgcccgg ttgctggccg
     8221 agaacccgat cctggcgcgg ctgaaggagt tggagctgct tcaggagatc ctcgccgact
     8281 cgaagcccac gttcgtgttc ggcaacacca acctgctgga tcaactgcgg tccctgaccg
     8341 gcaagccggt cggatcgtga aaccccgagg gcatccgact tcaccgaaag cgggtgccct
     8401 ctttcttggt cgttctgggg aattgcatcc cccggtattt gcacaccgga ggagttgctg
     8461 gagtttcgag atgattagag acccgatcgt tgaagatatc cgagaagccc gtcgcaagac
     8521 ggaggcggac tgcgaaggtg actgggacaa gctggtggag cattaccggc aggttgagac
     8581 gcacgcggga aagcgtgtga ttcagggttc gccgcggcga ccagcgacca caaggaccaa
     8641 cccgcggcag tgatggcatc cgtgaagtgc gatgcttctg gatcgctgat ggttgtttat
     8701 cgcccgtctt ggtttttccg actcttccag tgacagagca ccaggagtgc ccagaggatc
     8761 tcggtgtggt cggcgcggcg gcggcagtgg gcttcgtata gccgctgagc ggcttcgtgg
     8821 cggacgccga aatcctgcaa ggcctttttc gtgaccagat ccttgtacat tggggcaagc
     8881 tcgttgcgca ggaactcgcc gacgggtacc tcgaatccca tcttcttgcg attcagaatc
     8941 ggctccggca gtacgtcgcg gaatgcgtcg cggaggatgc gcttgccgat cgagccctgg
     9001 agcttgtact cgatcggcag cgatgccgcg aagttgacga cgtcaacgga cagcagcggc
     9061 acgcgaacct ccaagctgtg cagcatgctg gcggtatcga ccttgtgaag catgtcggcc
     9121 gggagaccga tggccaggtc ggccaggaga atacggtcca gcggagtcag ctcagccgcg
     9181 tccgctcgca gccgcgcggg agcgtcacga tagaggttga cgaggtactc gccggcgccg
     9241 gcagcgcgct cgggtcccag caattcggtc gcctgccgct gatcgatgat ccgagcccaa
     9301 gccagatggg tgccaagagg atcggccaaa tcgccgcgga gcagcttgcg cgcctgccgc
     9361 aggcgatcca ataggcggtt ggagcgggcc tgcggcgcca gcttgagcat cggctcgatg
     9421 acacccctgc gaagcacgcg cggcaggcgg cggtagcggg ccaggtagtg atggccggca
     9481 tagcgccagt acccggcgaa tagctcatcg ccgccatcgc cgctgagggc cacggtgacg
     9541 tgtcgccgcg tataacggct gaccagagca gcgggcagca gcgagctgtc ggcgaagggc
     9601 tcaccgagat ggttcagcac gggctcgacg gcgtcgagaa cgtccttgaa ggcgaggcga
     9661 aatgtgtgat gccgcgtgcc caagtgacgg gcgaccagtt ccgcatactc ggtctcgtcg
     9721 tatcgtggat gatcggcata gccgatgctg aaagtctcga ccggcgtcgg gccggcgcgg
     9781 gccagacagg cggtcacgat ggaggaatcg aggccgcccg agaggaacgc gccgagcggg
     9841 acatcggcga tgcgctgagc gaggacggct tcttcgacac gccgccggag ttcacaaacc
     9901 gcgtcggcat aggctggggg tggggcgggc agaggattca ggcggaagaa cggttccggg
     9961 ttcatcggcc cgcgtgcatc gaagaacaag caatgccccg gcgacagctt ttgcaccccg
    10021 gcgaagatcg tgcggggatg cggaatccag cccagcgtga ggtacagcgc aacggcctgc
    10081 ggatcgatct cccgccgcac ctccggtatg cggcaaagcg tggacaattc actggcgaag
    10141 accagtcggt ggttgtggaa cgtccagtag agcggcttga tccccatcgg gtcgcgcgac
    10201 agatgacctg accgccgctc gaggtcgaca tacgccattg cccacatagc atcaaaacgc
    10261 cgcagggcct ccgggcccca atgcatgcac gcggcgagcg ccacctcggt atcacaagcg
    10321 gtttcaaaag gcccgggaag ctgctcgcga agctcgcggt agttatagag ttcgccgttg
    10381 taaacgatcg cgcggcgatg actgggatcg gtcatcggct gatgaccatc gggcgtcgga
    10441 tcgatcaccg ccagccgggt gtgggccaac cccactgaaa agccgggtgt ctgggccgcc
    10501 cagatgccgg tgtcatcggg cccgcggtgc gcgaggcaac cgcagagatt ccgaagcaaa
    10561 tccggatcca tgagccggcc ggcaaagtcg atgatcccgg cgatgccgca catgccaggg
    10621 cattgtcgag accggaaaca ggaaaggcaa gtccgagccc gccgaggatg aggccatcat
    10681 ccttgaaagc cgaaaaaaag cggcaccccc gagggcaggc cctcgtggaa gccgctgact
    10741 ctcaccgccg gttctacaaa acacgaaccc tggcggactc aactcccatt tgcagcacga
    10801 tgatggagcc gggacggtat tgagttttgt cgagggtgaa acccggttga ccttccagaa
    10861 tccgtccgcc ggcgaagccc ccatcttcat cgtatcgctg ctcctcgatc acctcgatca
    10921 ggtcggggat ctccacgaga atgtagtcgc agaccatctc gtcggccggg cactcgaaga
    10981 ccgacgtaag cccatcgatg cgcagggtca cccgctcgtc atagccggca gtctggttgg
    11041 agaggcggac ggtaaccgtg ttttccccgg tatcgagacc gcgtcgctcc acaaggtacc
    11101 gcagaacctc attcacgtcg taacgacccg cgcagccgct gctcaggagg ggcagaccgg
    11161 cgccgattcc catgcccaac aaaggcacca gtagacccaa ggcggaccgc ctttttgcgg
    11221 gccgcaaccc aagtgctctg cgatccagaa acatgcgcgc aaaatgcgca ccagttgcga
    11281 cggaagggac taaacacttg cgggcaaaca cgttgtcaga ccgcctttcc agttgcaggt
    11341 ttccagctgt cctcggcagg aagccgcccg gcagctcggg gatctgcccc cgccgaccga
    11401 cccgggcgtc caaacccacc atcactttaa cgaccccagg tgcccgcggc aacccggcag
    11461 ggtcgtgaga accggtcctg gacggggaca tgtctcccgc agaaccaatc gggacgagcc
    11521 gccgaccggc cctggcccgt gcccgtcagc cctattggcg ggtaccatat gcactccaga
    11581 gtcccatact gtttgaggag gttcaacatg tatagaccga tttttcgagc tgcatgcaac
    11641 cgccggatgg cgggtctcgg ggcagcgatg ctatgtctgc cttgcatcct cgcgggggcc
    11701 gccatggcgg ccgacttgcc ccaggtcacg atagatagca cggccgttac gcttcacctc
    11761 ggcggccgcc cggtcgggcg ctaccggtac gcggaggtgc cgttcaagcc ctacctgcag
    11821 gagcttcgca cgccatcggg catcaacatc cttcgcgacg cacccttcga ccacctccac
    11881 catcacagcc tgatgtttgc cgtggcagtc gagggggtga acttctggga ggaaaaggac
    11941 ggtttcggga aacaggagca tctggcaatt cgcgacgtgt tcgcggggca tgccggcggg
    12001 ttaccctgcg cgtcattcaa agaggaagtg gcctggcgag cgggcggcaa gggactgctg
    12061 cacgagacgc gggcggtccg cgtgctggcc gatccggcgg gcaaggcctc ggtggtgtca
    12121 tggaccgccg agttccgcga actgaaccct gacgggcgaa cggccggggc cggccagccg
    12181 gccgtgacct taggtggcga gcgatactac ggcctgggca tgcggttcat cacctcgatg
    12241 gacggaaccg gccagttctt caacgccgac ggtgccaatg gcgtcgacgg gaccaacgac
    12301 aagcggtcac gctggtgtgc ctacaccgcc gaggccgacg gcaaacccgt caccgtggcg
    12361 atgttcgacc atccaaccaa cgcacgccac ccggctaact ggttcacgat gctcgagccg
    12421 ttctcgtatc tctgcgcgac gctcggcctg cagcacgaga aactggccct gggccgcgcg
    12481 gcctcgcagc cggccgatca acaagccggt gccgggcctt tccgcctcac ctacggcgtg
    12541 gccgtgtggg acggcaaggt acagcccgac gaaatcgagc gggtgtatgc tctctggcta
    12601 aagacaaccc cggcgcaaga gaccggtgaa agcaccaacg tgaaggccaa gtaaggttgg
    12661 caacttctcc tgcggtcatc agccggcccg cgccgggtaa gattgcacgg atcgagaccc
    12721 cggcttagac tcttgctgct tgtcgaattc gtcgtcggcg agccggcatc cttgtggttc
    12781 attcttcagg aggctcacgc atgagccaga ccaagcccgt tgattacgca gcggtttcga
    12841 ccaagacccc accgggtctg ggtatgggcg tccgcctttc cgcgatgatg ttcctccagt
    12901 acgcgatctg gggaatctgg gccccgattc tcgggttgca cctgggggct ttgcccggct
    12961 tccagacccc tgagggagcg gcggacttcg gacgcattgg cctgatctat atgaccatgc
    13021 caatcgcctc catcatcgcc ccgtttattg gcggccagat cgcggatcgc tatttctcgg
    13081 cggagcggtt tctagccttc agccagctac tgggtggagt tgtcctgctg gtcgtcagtc
    13141 agctcacgac cttcgccgga gtcttcatcg gcatgctggt atataatctt ctatatgccc
    13201 caactatcgc cttgaccaac tctatcacct tccagcactg gccgaaccag caattcagca
    13261 aaatccgcgt cgccggctcg atcggctgga tcgtcatcgg ctggatcttc accgtttgga
    13321 tggccaagct cggccatgcc ttccggacgc ccgcgatggg cgattgcctt tacggcgctg
    13381 caatcctgtc tatcgcctac gcggggtacg ctcttttcct tccccacacg cctccctcca
    13441 agcaggcggg caacccgctg gctttcctcg acgccgtgaa gatgctgagg aaccccgcgt
    13501 ttgcggtcat ggctgtggtc tcgttcttcg tggccatcga actccaactc tatttcatct
    13561 gggcacagag ttttctgaaa tcgggggtcg ggattcccga gggctgggtg agtaccgtac
    13621 tgacggcagg gcaggtgtgc gagatggtga tgatggtgct cttgccgctg gctctgctga
    13681 agtggggctt ccgcgcaacc atggcgatcg gtatcgccgc gtggtgtctg cgagatctgg
    13741 tctttgcggt cggccaacca acctctctgg tggtgggagc agtcggccta cacggcgtgg
    13801 gttacgcgtt cttcttcacg acgatcttca tgttcgctga tgcgatcgct cccaaggata
    13861 tcaaatccag cgcccaaggc tttctggcct cggtgacgat cgggtgtggc atgctcatcg
    13921 gttcgctgct ggccgggccg atctcgaccg cctgcgacaa ccagtggagc aagatcttcc
    13981 tggtgccggc gatcctgtgc gcggtctgct gcgtcgcgtt cctgatcggc ttccgcgaga
    14041 agcgcgacca agctgcggtt ccggcttgac ggggatctcg cgagagcagt tgagcctcgg
    14101 gtcacaggcc aaggcgggca tccgcgcggg cggaaaggcg tcactctggt ttgagcacct
    14161 gaaatgcctc ggcggccgca gccagcgtct ggtcaatctg ctcctctgta tgggccgcag
    14221 agacaaagaa cgtctcgaac tgcgagggcg gcaggtaaac gccgcgttcc agcatggcat
    14281 gcacaaactg ggcataggcc cgcgtatcac tacgggcggc gctggcatag tctgtgaccg
    14341 gtccgacctg gaagaacaac gtacacatgc tggccacgcg tgtgacgaaa gccggaacgc
    14401 cggccgtggc cgcagcagct ttcaaaccgt cctccaagcg agtggccaag gtatccagcc
    14461 tttcatatgt tccgggctcg tgaagcgcct cgagggtggc tagtcctgcg gccatggcca
    14521 atgggttccc cgaaagcgta ccggcttggt acaccggccc cacagggctc agcttctcca
    14581 tgacgtccgc acggccacca tacgctccta cgggcatccc gccgccgatg atcttgccga
    14641 ggcaggtcag atccggccga accccgaaag cctcctgggc tccgcctacg gacacgcggt
    14701 agccggtgac gatctcatcg aaaatcagga gggctcccgc ctcgtcgcac agttgccgaa
    14761 ggcccgccaa gtatccgtcc actggcggca cgcaacccat gtttcccgcc accggctcga
    14821 ccagaaaagc ggcgatacgc gacccgtgtc gctcaaacac ctcacgggca gccccaaggt
    14881 cgttgtatgg caccacaagc gtgttcgccg tcattgccgt cggcaccccc gccgaggatg
    14941 gggtgccaaa tgtcgccaat cccgaacccg ccttgaccaa gaggtagtcc acatgcccgt
    15001 ggtagcagcc ttcgcatttg accacaaggt cgcggcccgt gaagcctcgc gccaaccgca
    15061 ccgcactcat cacggcttcg gtaccggaat tgacgaaacg tatccgttcg atggacggaa
    15121 aatcctcaac gattcgctca gccaagcgcg tctcggcttc cgtgggggcg ccgtatgacc
    15181 agcccttgtc caaagccttg ctggcggcgg cagcgacgcg ctcgtccgtg tgcccgagga
    15241 tcaggggccc ccaggagcaa acgtaatcta tgtagtcgtt gccatcgaga tccgtcagtg
    15301 tcgctccctt cgccttggca atgaaccggg ggacaccgcc gatcgcccca aaggctctga
    15361 ctggtgagtt caccccgccg gcaagaaccc tgagggccgc cgtaaacgcg gcttgcgact
    15421 tcttgttctt ttgtgtctca tcggtctcgc ttttctgctt ggccatgatg gtgagaatca
    15481 taacggttcg gtccgggcct cgcgagtggc tgcatatggc gctctgactg gctgtggacc
    15541 ctacattttg tggctggttt tttggctttg gctcggtata atctctgcct cgttgggcga
    15601 tgtgattgcg tcccgtaagt atttgtgacg taacaactaa cgtatcctgg gtcgcgcgcg
    15661 gcgcagcagc cgatccggcg acaccatcgc aagtatctca ccgccgatga ccagcatcag
    15721 cgaacgtgcc tggaacgata ttcttgcctc tgtgaaggcc tccaacccgg agttggcccg
    15781 cccgtggttt gccgctctgc aaccgctcga catgcggcac ggcgtgatcc gggtgaacac
    15841 cacaacgcca gagcagcacc actatctcac gcatgactgt cagcaggcct tcaactccgc
    15901 cgcccaatca atcaccggcc gcttggtaac ggtacacttc gtgtcacccc cgacaccggc
    15961 tccgaggatc gagccactgg ctttcgaacg cgagaccgag agcattgtcc tcaacggcaa
    16021 ctacacgttc gacaacttcg taaccggccc ctgcaaccgc ttggcccacg ccgcctgtgt
    16081 tgccgtggcg gagaacccgg gcagtgttta caaccccctg tttcttcatg gcgctgtcgg
    16141 cttgggcaag acccacctgc tgcaggccgt ctgccaccac atccggtcgc agcagccgga
    16201 gagtaagatt ctctacctgt cctgcgagac atttaccaac cacttcgtcg aggcgatcga
    16261 gcggggtgcc ctgcaccagt tccggtatcg ctaccgccac gttgacgctc tcgtgatcga
    16321 cgacgtccaa ttccttgctg cccgagagcg aagccgagag gagttcttcc acacatttaa
    16381 caccctcttt cagttgcaga agcagatcat cttatccgcc gacgaatcac cgcgggacat
    16441 tccgagcctg gaagatcgac tcgtgagccg cttcaactcc ggtctggtaa cccgacttga
    16501 ggctccctgc ctggagactc gaatggctat cgtctctcac aaagcgaagc ttcgctgtgc
    16561 cgatgtcccc gaagatgttg ttcacttcat tgccgcctcg gttgattcaa gtattcgtga
    16621 actagagggg gccctgacgc gcgtcttggc cgtggctcag cagtatggcg ggacggtaga
    16681 cctaggagta gcccgtgagg ccctggcaca gagcccggct gccggcaatc gacgaattac
    16741 gatctctgac atcctccaga tggtaacgga acggtacaac gtccgcctca gcgacctgca
    16801 gggcaagcgc cgccatcgtt cgctagcctt tccacgacag atatgcatgt acttggctcg
    16861 cgaactgact cctctgagcc tggaagaaat tggcggccat tttggcgggc gcgaccatac
    16921 taccgtcctg cacgcaattc ggacaataag cgagcagcgc agcgttgatc aggagctgga
    16981 agcaaacatc cgccagataa cggccctgct aagcaagcaa aaagcctaac ctgcgccggc
    17041 gcggccatgc cctaccgagc catcctacca cgattcacgc ctcctgagtc gccgaaccgt
    17101 aggcgagtca gctttttgcc agctacaacc ccagcgcgtc atcactcgat aacgcaagcc
    17161 cccagcccgc ctggccggat gattctagtc accgccggta tcagcaggat cgtgatcccg
    17221 ccgccagtcc tgtggataaa ctgtgccgtt tccagtatcc tatcgacggc ttgctcctgc
    17281 ccggcgtctg gggagccaag gcccccggca tggcctggca cttggacagg ctgcgctgac
    17341 agcttatcca cgaccagcag accccaggcc ggagcggtaa gcccttgtgg aggctccaca
    17401 tcgcggaccg gcgttcgcga tggacgcgca attcacagga cacacgaaga tgatctgatt
    17461 agatagacaa tagttattag tcagcagtta gttgtgactc catcaaccta gtacggaagg
    17521 agcgagttga ccatgcaagt gaccgtcaac cgcaacgcgt tggtcgaagt gttaaacatg
    17581 gctgccccgg ttgcagcaag ccgcacgccg cgggaattgc tgaagtgtgt cagactgacg
    17641 actgtcgacg gctcgctgct ggtaggcgcg acggatctgg aagtggcctt gcgcggcgag
    17701 gtgcggcagg ttgaagtcaa ggtacctggg gctgcgctgg taccggccga taagctgatg
    17761 cagattgcac gcgaatgtcc ggacgagacg ttgaacatcg agagcgagga gttggtgtgt
    17821 cgcattcgcg gtgcggacag tcactttgaa atctacgggc aggatccgaa ggatttcccg
    17881 ccggtgcctg atcttgaggg tacgcctgat gttgaagtcg agggctcggt gttgacgtcg
    17941 ctgattgagc gaacgctctt cgcggttgct aaggagaaca cgcggtacgc catcagcggt
    18001 gtcttgtggg agacaaaggg gaaacggctc tcgctcgtgg cgacggatgg tcgtcggttg
    18061 gcagaggcaa acgggccggt ggcgcggagc accggtggtg accagcgtat gattgtgcct
    18121 gccaagacga tgcaggtcct gcagcgggtg ctgccgcatg tcccggagga cacgccggtt
    18181 ggggtccgct tctcgagtaa tcaggtggtg gtccgagcgg gattgtttgt ggtcagctcg
    18241 ccactggtgg aagggcagtt tccaaagtac gaagacgtca ttccgaagga cagcgacaag
    18301 aagattacgt tatcaacgcc agactttcac agcgcggtcc gccgggcagc tctcttggcc
    18361 agtgatgagt cgaaaggtat tcgtttggcg tttgacgcgg ggatgctggt actgtcaagc
    18421 cggaccccgg aacaagggga agcaaccgtg tcgatgcgca ccgactatca gggtcaaccg
    18481 cttcagattg gcttcaatcc tgcctatcta gcggaggcac ttcgcgtggt ctcctcgccg
    18541 acagtacaga tggagctgaa ggagggaaat cggccgggca tcttgagggc aggggccgag
    18601 ttcctctacg tgattatgcc cgtcagcttg tagctgggga tgggccaggg gagcgagcgt
    18661 gaacgacgag cagttgagat gggttcagta caatcacggg gaaaccgggg cgcgcgcgcg
    18721 gccgccggta gagactgagc ggacgcgcca gcgggaggag cgggaagcag cacgacgagg
    18781 ggcggtaacc cagatcggta agctggtccg ggcaatggtc gagaagaggt ccggcctgac
    18841 ggcacttcag cagaagttgc tggctatctt ggacgaagag ggtggaatcg acttggcggc
    18901 gcaggttacc ggcctgggac tctatcaggg aattctgcgg atcgagacag ccgatccggt
    18961 gacactctgc gatctccgca tgcgctggca gaggcgcttg ttagaggcag tccagagccg
    19021 cttaccggag gcgggcattc gtgcggtccg gttcagtttg gcagggcgca gtagtcagag
    19081 cgactcgcaa gaataggata gacggaaacc atgaccgagg ccgccagtga ggcacaggga
    19141 gttccaacgg gggagccggc tgagcaatcg gcaacgccga cggagagcag atacgacgag
    19201 tcaagcatcc gtgtgatgga gggacttgag gcggtgcgca agcgcccggc gatgtacatc
    19261 ggcgacacgg gcatcacggg cttgcaccat ctgatctatg aggcagtaga caacgcgatt
    19321 gacgaagcga tggccggcta ctgcacagag atcgccgtga cgctgggagc ggacggcagt
    19381 tgcgcggtgc gcgacaacgg gcgcggcatc ccggtcggcc cgatgaccca ccccgacccg
    19441 aagatcaacg gcaagcccgc agtcgaggtg tgcatgaccg tactccatgc cggcgggaag
    19501 ttcgaccggc gaagctacaa ggtctcggga gggctccacg gcgtaggcat cagtgtggtc
    19561 aatgcgctgt ctgagtggct tcgcgtccag gtgcggcaag gtgggaacgt ctatgagatg
    19621 gggtttgagc gaggagtaac gacgtcgccc ctcaagatca tcggccatgc ggtcgacacc
    19681 ggcacggtcg tgaagttcaa gcctgatgca cagatctttc ctgaccacga tttccgttat
    19741 gaaacacttc gatcgcgact gcgagaactc gcgtacctta acgagggagt gaggatcacc
    19801 cttaccgacg agcggaccgg gaaatcggaa gtgtatcatt ttgctgatgg tctgcgcgag
    19861 tttgtggcgc acctcaacga aggaaaggag ccgatccaca aagacatcat cctgatgcgc
    19921 gcccaggacc aagagcagcg cctcgtcgtc gagatcgcca tgcagtggaa cgacggctac
    19981 aacgagaacg tctcgtgttt cgcgaataac atccgcaaca tcgacggcgg aacgcacctg
    20041 tcgggcttcc gtgcggcgct gacgcgaact ttgaactatt acgcaaaaaa agagaacctc
    20101 gtgaaaggcg acctcgtaac gaccggagag gactttcggg aagggttaac cgcgatcgtg
    20161 gcggtgaagg tgcccgaacc gcagtttgag gcccagacga aggtccggct gatgaatccg
    20221 gaggtcgaga cctttgtgac ccaggtggtc aactcgcaac tctcgacgtt cctggaggag
    20281 aaccctgccg acgcaaggcg gattgtgctg aagggcatcc aggctgctca agcccgggag
    20341 gcggctcgga aagcccggga gctgactcgc aagagcgccc tctccagcgg cgggctgccg
    20401 agcaagctct gggactgccg ggtgaaggac gctgatacgg ccgagttgtt catcgttgag
    20461 ggccaaagtg ccggaggcag cgccaagacc ggccgtgaca gcgagttcca ggcgatttta
    20521 ccgctgaaag gcaaaatact caacgtggag aaagcccgaa ttgacaagat gctcagtcac
    20581 gaggagatca agacgctgat cctcgccctg ggttgcggca tcggggaaga cgagttcgat
    20641 atcagcaaac ggcgatacgg caaggttgtc ctgatgtgcg atgccgacgt cgacgggagc
    20701 catattcgta ctctgctgtt gaccttcctg tttcgccaca tgcggccgct gatcgaaggt
    20761 ggatacgtct acgtcgccca accgccgttg ttcctgctga agaagggcaa gaagagcgag
    20821 tacgtcctga acgatgccgt gctcagccga aagcttaagg aatgggggac tgaggacaca
    20881 acgctggtgg tgcgggccgc cgacggcacg gagcgggaga tcgccgggcc ggagctggcg
    20941 gagctctata cgctgcttga gcgaatcgat gcgcaacggc gcatcctcga gcgccgggga
    21001 atcgatcagc gagagttgct tacgcgacac gtcgatgaac aggggcgctt gccggcctat
    21061 cgagcggtgg tctacaggcc gggcgatccg gagcccgcca cttacttcgg ttacagcgac
    21121 gaggagttcc aggcattctg tgaacaggag cgacagcggc tcggcgaggt tgaggtggtg
    21181 gaggccggcg cgggtatgat tccgccgaag aacaacggca acatggctca ccggattatt
    21241 cgctccgatc tgagcgagtg tgcggttctc gaggaggcga tgcgcaagct cggggcattc
    21301 ggcctgagca tggatgatta ctacgccgag cgggtggagc acgtcgacgg cacggtggat
    21361 cccgcgagat tcgtgctcaa gcacggcggc caggttgtcc gggaggtaga cgggctgcga
    21421 caccttgttg aagccatccg ccaagtgggt agcacgggag tggaggtgaa acggttcaag
    21481 ggtcttggcg aaatgaacgg tgaggaactc tgggaaacga cgatggaccc ggagcggcga
    21541 agcatgctca aggtggtgat ctccgacgag ccggacgacc cggagcagtt ggccctggac
    21601 gcccgtgagg ccgaccgcat gttttcaatc ctgatggggg acaacgtcga gctccgacgc
    21661 cagtacatcg aagcaaacgc cgcacacgtg aagaacctgg atatctagga cacggggccc
    21721 tattgcgggg cgcaacgcga acacgccgtg atccggaaag tcccgacatg agcaacaacc
    21781 gaatcccttg tacgacggca acgtttcttc tcttggcagg aagtctgaca atgggagcat
    21841 caccgggccg ctgggcctac cgagaaggcc ggttagataa tggtttgcgc gtcctgacgc
    21901 tcgaggacca tcgttcgccg ctggtttgcg tccaggtttg gtttcacgtg ggcagcaagg
    21961 atgaagaccc gcagcggcag gggttcgccc acatgttcga gcacatgatg ttccggggca
    22021 cggaccgcat cggcccgcag gaccattttc ggtatctaaa tcgcttcggg gcccgcgtga
    22081 acggatacac cagcttcgac gagacggtgt actgggaggt cctgccggcg tcgcagttgg
    22141 atcttgccct ctggctggag gccgagcgca tgggccggct gaagatcaac gaagaatact
    22201 tcgcggccga gcgaaacgtc gtgaaggaag agcggcggat gcgttacctc aaccggccgt
    22261 atggccgatt gtatgagacg ctgttcgacg cggctttcaa ggtgcatccc taccgctgga
    22321 cgccgatcgg caacatcccg catctcaacg ccgcgacggc ggaggagctc caggcgttct
    22381 tcgagaagtt ctacgtacct aacaatgcga ccctggtcgt ggtcggcgac gttcagcatg
    22441 acgacgtcat ggccaaggcg acccggttct tcggaaccat ctcaaggcgg ccggacccgg
    22501 tgcgggtcgg cgtcgtcgag cctccggtca ccgaaccgcg tcgggtggaa atcgcggatc
    22561 gggcaccgtc gccgcgcgtc gtcattgctt atcatacgcc gggcgcccgc gacccggaca
    22621 gcttggccct tgaggttctg tcgaaaatcc tctcctccgg tcagagcagc cgcctgttcc
    22681 ggcagctcgt ccaggagagg cagatcgccg tgagcgtgtt tggatcggcg tacacgctag
    22741 agcaggcggg cttgtttgct ctatcggcga tactgaagcc ggaggtctcg atcgaggagg
    22801 gcgaacaggc tctgctggaa gagatccggc gtctgcttga gcagggtatt gaaccggccg
    22861 agctggaaaa agcccgcaat cagacactgg cggagtacgt acagcggggc gagacggtgc
    22921 aggggcgggc ggatctgctc ggctatgcgg cggtgatcct gggtgacccg gagcgggtga
    22981 acaccgatct ggagcggacc cgggccctga gcgccgagca ggtcatggcc gtggctcggc
    23041 gagtgttcgg cgagcaaggt cgcgtgaccg tcgtgatacg ccccgacccc aatccgccgg
    23101 cggacgacga agaagacacc aaggcgcctg aggaaaagcc gattccggac ctgccgccgc
    23161 caccggagat gcctcagggc gactcgccgc ggccggtcaa actggcgtca ccggttatga
    23221 ggcagcttga gaacggcttg cgtgtggcgg tgttttcgga tcactcggtg cccgcggtga
    23281 cggtggcact gaacgtgctt gtgggggccg caagtgatcc gccggaagcg gcgggcttgg
    23341 cctacgtcac ggcgaacact ctccgtcgcg ggacagcccg gcatagcggc gacgagcttg
    23401 cggaaatcat tgactcccac ggcatgaccc tgagcgagtc ggtcgatcat tatgagagcg
    23461 ggatacggat gtggacgctc tccgagcatg tccaactggc gtgccggacc cttgccgaga
    23521 tcgtacgaga gccgtcattc ccccagcgcg aggtgaagaa cttcgtcgag cgggcggttg
    23581 cccgagagag gatcgaggag gagaaccccg ctacgatcgc cggtcggacc cttgatgaag
    23641 cgatattcgg cgacaacccg ctcgctcgac cggagggcgg cacctcgacg tcattgaaag
    23701 ggatcacgcg ggaccaggtt actgcgtttc accggcagtg ttacgcccca aacgtcgcaa
    23761 cgctggtgtt tgccggggat gtcacggcgg agcaggcgac cgcaatggcg gaggaatgct
    23821 tcggcgggtg gggtggtcgg gcgaccttcg gggacccgcc ggcaccgccg ccgcgaggcg
    23881 atctcaaggt gctgttagtg gatcgcccgt cagcctcgca gagcgagatc cggatcggac
    23941 aggtggtgcc ggtatcacga cgtgatgggg actacgcggc cgtgcggctg ctgagcatgg
    24001 ttttcggcga gtcgttcagc gcccgcatcg ggcgcgtgct gcgcattgaa aagggcctga
    24061 cctacggggc ccgcggctct ttcgaggtcg agggcaacgc agcggcgttt cgcgtcagca
    24121 cgttcacccg aacggaaaag actgccgatg cggtccaggc ggcccttgcc gaggtggaca
    24181 agcttctcac cggtgacgtc agccgcgagg agctggccct ggcgcgggat cagttaatcg
    24241 gcgggtttca ggttggcttg gagaccccgt ggcaaacagc cagcagatgg tgggacctca
    24301 aagtctgggg cctgccggag ggctggtatg aggactacca ggcggcgatc gtcagggtga
    24361 acgatccggc ggtcttgcgc caggcggccg cgcggaccat cgagccgagg aagctgacca
    24421 tcgtggtggt gggcaacggg gagggggtcc gcgaggggct ttctcgcatc gcgccggtag
    24481 aatcggcccc tgctccatga gtcgcggggc atggtgatga acagtcgact gcggggcggt
    24541 ccgctttcgc aggaggttgc cagtgagcga gggaccatcc ggcaggcgag gagcgggtgg
    24601 ggctgttttc ggcgcgctgc ttggcgtcaa cttcgcggca cttgtgctgt tgggcggtgt
    24661 gcttctggtg ctgctgccga agatggctgc ggtatacgct gctttcggga caccattgcc
    24721 cgccgtgacg gggatcttgc tgcaggtgtc gcaggcccgg gctgcggttc tgatggggct
    24781 tggtctcctg cttgcagggc aagtgacctt ggccagggtt gccggccgtg gccgccacgt
    24841 tgtgctgctt gtcggtgtcc tggtggagat cgttctcgtt gttgcggttg ccttcgccgt
    24901 gtttgtgccg tacgtcgagt tgattaacag catttcgggt tcgaatggag caggcggtta
    24961 gttgcttggt cgccggcggg gctctgttct gctcgatcag gccgggtgcc ggattcgtcc
    25021 cgcctgcccg tggcattttc cccttgtcag gagacaattc acgaggtaca attatttgta
    25081 tcttgaaacg gcgttcgcag acggtcttga ggccactgtt ctaaggcgga ccgccttttt
    25141 gcgggccgca acccaagcgt tctgcgatcc agaaacatgc gcgcaagccg cacagat
//
LOCUS       DAOTPW010000054        25123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2062_length_25123_cov_5.823759, whole genome shotgun sequence.
ACCESSION   DAOTPW010000054 DAOTPW010000000
VERSION     DAOTPW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 25123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25123
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2062_length_25123_cov_5.823759"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="P5316_06855"
     CDS             complement(<1..685)
                     /locus_tag="P5316_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR84713.1"
                     /translation="MLRQITTLLRPAPQIDRIRDAAKVERQYAYWRQRVLYASLIGYA
                     VFYLVRKNLPVAMPVMQEAMGATKADLGLFLTLHGILYGVSKFANGFLGDRTNPRWFM
                     ALGLLLSAMMNIFCGFTGVALTFGVFWVMNGWFQGMGFPPCARSLTQWFSPAERGTKF
                     ALWNTSHSIGAAAALGLCSLLVTHDWRLCFLVPAVVAVAGAVFLVDRLRDSPQSLGLP
                     SIEAYWDMHE"
     gene            complement(698..2089)
                     /locus_tag="P5316_06860"
     CDS             complement(698..2089)
                     /locus_tag="P5316_06860"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HRR84714.1"
                     /translation="MSCEQRAQVACEERFAERVERALRTASDTREVQIGEGVIALTAE
                     VFRRQFPESAAVVVADRNTLRAAGHRVLGLCRQAGLPVTEPVVFEDPDLHADYRHVER
                     LQSFLNGGGAVPIAVGSGTINDLVKLAAHLCGRPYMVVATAASMDGFTASGASITRHG
                     LKETFFCPAPRAVIADLDIVSEAPGELNAAGYADLAAKTTAGADWILADALGIEAIEP
                     GAWDLVQRCLRQWLMNPEAIQKGNRIAIRGLLEGLLMTGLAMQHCRSSRPASGAEHQF
                     SHLWDMEHHRHAGTVPLHGHKVGIGTLAVTMLYEELLRYPLQLMDPQRICDRQPDLES
                     VRRQVRQTHTLPELRDVSLREVEAKHPDRKQLLALLQRLREVWPDLRERLRDQLIPSR
                     VLCRMLQQAGAPCRPEAIGIDLPRLRRSYLAAQQIRRRFTVLDLATLTGLLPECLERL
                     FARGGAWEQLGHE"
     gene            complement(2086..3027)
                     /locus_tag="P5316_06865"
     CDS             complement(2086..3027)
                     /locus_tag="P5316_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRR84715.1"
                     /translation="MNCFEMAASFCGGARRLVGRGFSAVVLLAWTTGCSLPGDGINQT
                     SGSCPPEFIIQSHRGAGKLAPENTMQAFELAWRLGTVPEADVRTTKDGVVVAFHDATL
                     DRLVSDLPPEFQGKGTRDLTWSELSKLRIGPQGQPIPRILEVLDAIRGRPERWLYLDV
                     KEVPLERLAEMVSERGVASQVILASTKYEETRRWKELLPRSQTLLWMGGSEAGLSRRL
                     KEVKAADFIGVTQLQIHVKVGNLASEDTFQPSAAFLRAIGEELKARRILFQVFCPGGC
                     DAAGYERLLQLGVQSLATDDPEAALEAVRNHKKRSKK"
     gene            3373..3972
                     /locus_tag="P5316_06870"
     CDS             3373..3972
                     /locus_tag="P5316_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HRR84716.1"
                     /translation="MKKNNRSDDIRQWLTRPIIPVVSQANADTALVAEASLLFLQGCE
                     LADLADILGWLKKGPLAHLPIMLHIDLLAGLSSDDAGLRYLAGLGQIDGIITVRPHMV
                     AAARKQDLASILLLFLQDGRAVDRGLHIVEQSEPDLIELVPGVAALEVLPQFANVPMP
                     RIAGGLIRTADLARRLIDAGCDAVSTSTADLWQLNRSTS"
     gene            4286..6226
                     /locus_tag="P5316_06875"
     CDS             4286..6226
                     /locus_tag="P5316_06875"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRR84717.1"
                     /translation="MTCERARIVLWAVAAAMLIVGPVTGQMNDGAEAESEQAKSAPAD
                     ETSGGQVIEGLVLNYYGGGITGAQIRVEALNAKPGDPPIAEGKSGKGGEIRIRLPKRI
                     DENVLVRIRMDGYQEFVQEVDPTDPENPPFVDAALQGAGTISGVVRAKSSGKPVANAK
                     VLGASGGRDTSARTDANGKYTLTGIVRGRAYLKVIADGFATFQTILNIDRQDSVQDVE
                     LAAEHPVELLVITGDGDPADKVLIEGWIESTKSSIETITDAQGRATLRGIGENVEEIR
                     LRLNGDRYVRMSGFDERIDLAASQPATETRPATTRPSRHKIIVRIGARVRGKITNDKN
                     EPLAGVRVTAGRKNLADMPVTWTDLDGEYELQGLPAGLITMTFQHPDHAPDVQEISLN
                     AGQTGTLNARLTTGGTVAGTVVGPDDKPIEQAWVVLEEWKGYQTIGMRAVTDEQGRFS
                     FKSVPEGDLIFTIVKSGFGGPIRQTMAAGKTDYRIVLEETTPPAVTGGPGPQEDKIGV
                     GQAVPDFTLVGVDGTKYKLSELKGKYVFLDFWASWCGPCQREMPHIKALHEAMKNEPS
                     FILIGVSLDGNEKDFKAAVSKRNIDWPQVWGPESGAEKTFEMLEGLGIPYTCLIGPDG
                     RMLAQHIVGPETTEQVKKLIRN"
     gene            complement(6376..7668)
                     /locus_tag="P5316_06880"
     CDS             complement(6376..7668)
                     /locus_tag="P5316_06880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR84718.1"
                     /translation="MAAGSVPSGKLIVFALSALLMWPPIGCSEQAGGVEKPPVTTTPA
                     SQPASERVLIRIGDKAVVTQADVEEILRTTPPERRRGSAYKALGTALTNKLFLVYLDE
                     HPDLVTQEDIDREIDKDLKIAKAKTTEEMKMILQSSGVSWDEYLRQLRIRLGQGKLSK
                     IGAARVKDDEYLRQIYDEHPDYYDDTRVKLRHIVFASPLTATPEQKKATREALERLRE
                     EIVSGKKTWDEAVKLSNDNTRTKGGLLGTLPRYMRLNEVLISAAWALEPGQISNVVES
                     GIGYHIIQVLERVPGRRPFDDPHTKFQIRAVMQSRPLAEAMEEVKRKYPIIGVQPMDL
                     ASIMALPEPPAAPTASQPTRSSTRPAGGTASRPATRPATARPVTRPAGFRAATSPAAA
                     RPSTRSAPPRPAVPRTTAPQGLPGGVPGASPVSQPSGS"
     gene            complement(7765..8472)
                     /gene="plsY"
                     /locus_tag="P5316_06885"
     CDS             complement(7765..8472)
                     /gene="plsY"
                     /locus_tag="P5316_06885"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRR84719.1"
                     /translation="MHVIESGWWPVAGLILLSYLCGAVPFGLLVGRLKGVDVRTKGSC
                     NIGATNVGRVLGRPYGILVFVLDGLKGFLPVTLGRLLLFGAAADSRLAGPGYLPYLLW
                     VLAAVACVLGHMFPVYLKFKGGKGVATSLGVGLGIYPYYTLAGLAALTLWAVVLWVGR
                     YVSLSSIVAVTAFPVIFALLAILNRDRWGGFSVLWPLQVFGIVIAALVVYRHRSNIGR
                     LLAGTEPKIGGSPHRRE"
     gene            complement(8531..9712)
                     /locus_tag="P5316_06890"
     CDS             complement(8531..9712)
                     /locus_tag="P5316_06890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR84720.1"
                     /translation="MNVALVIERIEAWRGGAETSTVQFARHLGSIGCNVTVVTMTDVP
                     STPAMSIVPIKVGAGWRAARTWRFVRGAAKYFRTHQFDIVHAIAPCPFADVYQPRGGT
                     VPEMLSRNLALRPSAVKRGLKWVGQRADLKYRLLAGLEKRLLTRRPAPWVIAISQYVS
                     NQLKRHYGFDAARIRLIFNGVDPDTSPAHERRRHRIEIRRQFGLADDDLVLLAVAHNF
                     RLKGVDKMIQAMADGRVRELGNVFAIIVGRDNPKNLVERAAATGLADRVFFAGPSQRV
                     SAFFHAADVLVHPTYYDPCSRVVLEAMAAGLPVITTRYNGAAERITDGREGYVIDCPT
                     DLAALADRIARLADADHRRHCAQAAPAAVEQFTVARHATEVMRLYEEVLRDRNSRVTD
                     R"
     gene            complement(9709..10569)
                     /locus_tag="P5316_06895"
     CDS             complement(9709..10569)
                     /locus_tag="P5316_06895"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HRR84721.1"
                     /translation="MFQTTVGCERLLSGCGLGDLDAVFRWHQGERLDKPGLEPWRQRW
                     RIALGHQASAAEVLYLKRFLNPPLKRQCARWLEGNWRLSTAGIEWRNAQDLAAAGIPA
                     AEAVAFGQEMMGPWERRSFVVLRAVEGTSLEKWVPRYLSRTSAGADDQHRRERIDRLA
                     RFVAEFHRAGFVHRDLYLSHVFLEPQIGKPGESQCEGYRLIDLQRVFRPRWRLCRWVV
                     KDLAALHYSTPADCVTTTERLRFLARYVRCCTRFGSARRLAAKVHAKTRNMLGRRRKV
                     VAAESSGPGR"
     gene            10838..12097
                     /locus_tag="P5316_06900"
     CDS             10838..12097
                     /locus_tag="P5316_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84722.1"
                     /translation="MQSGKSNGPSRRQVMCGAAVAAATTAFPYVLKGAAFDKPIKVGL
                     IGCGGRGTGAAHNAMEADPAVRIVALADAYKDRLEGCRGELKKRGAEIEDKHCFVGFD
                     AYKKLLELDLDYVILATPPYYRPEHFTAAIKAGKHVFTEKPVAVDPVGIRIMLEAGKM
                     ADEKKLSVTAGTQRRHQNSYIETIKRIHDGAIGKILSAQCYWNMGQLWYKEREPKWSD
                     MEWMHRDWVNWAWLSGDHVVEQHVHNLDIINWVLKTHARKAVAMGGRARRVTGDQYDF
                     FSADLSFPNPEAPDKENMDIHVHSMCRQITGCASSVSERVIGEKGISNCAGWISNAGK
                     LDIPGNEPYVQEHVDLIKAIRTGEQINETQNVSESTMTAIMIRMSAYTGKEVAWDDAI
                     ESDLKLGPPDYKLEEAEIRKHIPVPGK"
     gene            12597..13007
                     /locus_tag="P5316_06905"
     CDS             12597..13007
                     /locus_tag="P5316_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84723.1"
                     /translation="MQYVPQLLPYNKERIDEQRHLHGHYLLTASQNLLTMEQITESLS
                     GYELCDHRDIDARLCFRQTSTGAEVDIVVETEGRLVPIEVKLLATPQPAMAAGTLAFR
                     DAYGEGAAPGYVVHPGNIRLSLAPGVTAIPFAEL"
     gene            complement(13177..14319)
                     /locus_tag="P5316_06910"
     CDS             complement(13177..14319)
                     /locus_tag="P5316_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84724.1"
                     /translation="MTIRTGFGILILCQLASIAGCAAVKPSTPNVRREVPVAVLRSAP
                     PMELPGAAMPEKNVTGLVDCNNPVHWDGDAMYVFSSVGYADRSEGADLFSLKGPSVRT
                     RYDNDADPSIGGRWIEATFKHESGVLYGWYHNEPPQVCGPHKRLTAPRIGAAISFDNG
                     LNWKDLGIILTAPPDSLYCETPNRYFAGGSGDFSVILDQQRRFFYFLISTYNRDVAEQ
                     GVAIARMAFADRDHPVGKLFKWCKGAWREPGLGGHVTPVFPARSDWHQEKVDVFWGPS
                     IHYNAYLDQYVILMNRAIDTEWTQEGIYVIFCPDLSDPNGWATPVKVFDGRGFAPRQN
                     NWWYPQVIGIDASRKETDKLAGRVARFFIRGKSAWEIVFLRPGEKL"
     gene            14469..15455
                     /locus_tag="P5316_06915"
     CDS             14469..15455
                     /locus_tag="P5316_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRR84725.1"
                     /translation="MGIIADIPAIGTISEESGLIPLPATADGRFHVGRDGVVSVTATG
                     DRKVEFVAFAGHTLAHVKSAMGYAAYYPVHPVRLDPPVEAVLMDLDGTSVRSENFWIW
                     IIEMTTASLLDNPRFELEEADLPFVSGHSVSEHLQYCIRKYCPDKTVEEARRYYFEHT
                     HREMAAILAGRGRENAFTPTPGLKDFLRELKATGVKVALVSSGLHEKAWPEIVSAFRT
                     MNLGDPKGFYDAIITAGYPLRHGEVGTLGELSPKPHPWLYAEASRVGLGIPFERRHHV
                     IGIEDSGAGVCAIRLAGFAAIGLAGGNIAESGTKTLCMSYCETFEEIASYLR"
     gene            15591..18188
                     /locus_tag="P5316_06920"
     CDS             15591..18188
                     /locus_tag="P5316_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84726.1"
                     /translation="MPVNTITLDGDWLLKDFDHERGEAARPFDAACPTDDWLTTPVPG
                     DIHPTLVEAGRLPSDMFMGTNVEQCRWTGEREWWFRRDFDVPADFVLQRSELVFEGID
                     LFGTVWLNGHMVGQTRNSHRAYRMDVTDVIRPGQRNTLVVRIGATLRILEANPYQKYF
                     ACFNTPRVFARKAQCQFSWDWAPHLPALGIWRSVRLESFDSGRVMDVAVRARNDGHVT
                     FHMTLDERTCRQDLDQSSTEDRAAETVKPTGNLLVEITGPFNLAKPVKARPIRHGIPI
                     TGQKNFCTLCVPKPQLWWPNGYGQQPLYKYRITLIRNGRAHHQVEGRFAFREVRLVEE
                     PLGSDRLSFRFEINDTPIFCKGANWVPPSCFPGTLRPDRYRHLVHLAQDAHFNMLRVW
                     GGGIYESDAFYDTCDELGIMVWQDFMFACSDYPDDHPAFIDEVIPEIEYQVKRLRNRP
                     CVVYWCGGNEKTGSAGFKVSYGERLFHVIIRGVCTDLDPTRPYRPASPQSYTDLGNDR
                     NSGDSHGGCYEDAYQAGITCWREKLRDYNTVFNSEFGYHGPTRMQSLIKFVPREKIWP
                     ISEEMEYHVQDNPYNTIPETFAQVQAKMASTLVGEISDAEGFVRCASTFHAELIRAEI
                     EHHRRRKWENAGAMFWMFNDCWPCASWSVVDYYLVPKPAYYAGKRGFAPIIAAIIDEP
                     DHYAVYVVNDTLKPLAGTLTFGQGRVAGKPVWSRKCRVRVGANSAQLAASIRKKQVKA
                     TPDSYLFASFGTGARSLAETSFFHRPWREIHWPVPELSWRTSESIRKPAPLSYKPGPH
                     YVMRINFSTVNYARMVHIDGIESEGVLLSDNFFDMLPGSTKTVEIRSDHRIDPTAMRI
                     RHWLDRWEE"
     gene            complement(18760..19524)
                     /locus_tag="P5316_06925"
     CDS             complement(18760..19524)
                     /locus_tag="P5316_06925"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HRR84727.1"
                     /translation="MDCRHRQTGIGVLVCLVIVAGMVGASVKGLLGPWGSAGAPAPPT
                     GRLKVVMGRTPAPAEPLKRASLTVTRVEVLRQHTAPQLAENAGFLGLPQAAAAVAADQ
                     SWIVVQEGEQIIDLLEIQAGQANLLINADLPEGPYTSLRLTCREGRVTLDRPDHETPD
                     DTLVPDGNRRKAAQVTLDCGFMIAPGRETALLLDIDVNEAFEPVKVVDGQEAMLKGFR
                     FNPRTAMRLTHLLPAGTAGTTTAVPSTHQHRGDCSW"
     gene            complement(19836..20033)
                     /locus_tag="P5316_06930"
     CDS             complement(19836..20033)
                     /locus_tag="P5316_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84728.1"
                     /translation="MFNFGAMGSEIVRAECPECGLVNVRIESRLGRVQCIRCGHVFWK
                     SAALPTSELKAGWRRQARRDF"
     gene            complement(20438..20629)
                     /locus_tag="P5316_06935"
     CDS             complement(20438..20629)
                     /locus_tag="P5316_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84729.1"
                     /translation="MTIHIKGLPRRILDTEDATAGDVILSARDAFDGGADVDIDDIHR
                     AVGCIGGAAQSKALPARMP"
     gene            complement(20630..21709)
                     /locus_tag="P5316_06940"
     CDS             complement(20630..21709)
                     /locus_tag="P5316_06940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRR84730.1"
                     /translation="MPIRRRLFEGLSWRLEARAAASPAASGDRLRGCAGRMMALAGAH
                     NHLPDIEQQAGILCFHGITERPDPQVEDNVLSLRNFRRLLNVLHRSFHVISLTELVAC
                     IRERRSPPPKSIVVTFDDGYACCHELAAGELALRRMPWSVFLPAGLIETGGRQWLDDV
                     RVLIHRGGRKRIVLHWDGQAVEFDLSTPEHRSAAVKQIIEACRYLPEPQRRSRFDELL
                     SLYSDDEIAFLREQYPSFAPMTWQQVRELKLAGVDVGSHGLSHIALAPQTSDYIRREL
                     AAARDLLQERIGDHSPHFSYPYGRLAAMSSQTEVLIREMGYHCALTLEQEIVRCPSCN
                     LMALPRLIVSAQVGRVLFGLWQRFL"
     gene            complement(21754..22554)
                     /locus_tag="P5316_06945"
     CDS             complement(21754..22554)
                     /locus_tag="P5316_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR84731.1"
                     /translation="MLQLWLYRDLLYQLVRREVVVRYRHSLLGVSWALLQPLGMMLLF
                     TFIFTRAVSLNAIASLDVPYPLFVYIGVLPWTFFATGLNGSIQSLTSQRDLVTKVYFP
                     QQILPLTSIASAFFDFLVASVVLVGLVVYFDATSDWSFAFRSVLLMLPLVVVIQVVLM
                     VGLGLWLALGNLWYRDVKYLFAVGIQMWMFLTNVLYPLPTDGSAALRWLTTVNPMVPI
                     ISAYRDIVIHGRLPEAVPFLTALGVSVVLAASGWAVYRALEGRFAERV"
     gene            complement(22683..23915)
                     /locus_tag="P5316_06950"
     CDS             complement(22683..23915)
                     /locus_tag="P5316_06950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR84732.1"
                     /translation="MISGKNIICFASGWNFHPTSKHHIMRRLARRNNVVWVNWHASRR
                     PTMHPYDLRMIWDKLRQMRQGAQRVAHNIAVVTPFQVPMPSMRLARRFNRFTVRRAIE
                     QVVETLPNRPVQLWSFAPDVSDFVGCFGEELALYYCVDAFGEFPGYDRALIENRERQL
                     LDRVDVVIATSRPLYESKSRFHRNVHLVEHGVDYEQLSRAVKEELDAPRELESLRRPI
                     IGYIGVVGEWVDLDLLAALAREYRRASIVVIGPVLVPHGPCEDLPNVYWLGERDHVLL
                     PAYLRLFDIGLIPFKHVPLTRSANPIKLYEYLAAGVPVVSTGLPSVRPIPNAVWVADD
                     VERMIRCCEDALACNSAADRRRRSDLMRSESWEARLEQIAAIIDACGRTPPCGRSGGL
                     AEALAEEERCEAAVMTST"
     gene            complement(23912..>25123)
                     /locus_tag="P5316_06955"
     CDS             complement(23912..>25123)
                     /locus_tag="P5316_06955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR84733.1"
                     /translation="RFLAQRHDVWCAGFLETGFLGRTSQEARRSLGALRSMCREVAAV
                     PLHESLAQARALTGLLAGGTATEQYFASGRLRRQVMQWAEQVGFDAVLAFSSSMAPLA
                     LQVPASRRVLNLDDLDSRKWAESAAGAAWPMSWVYRIEARRLDRRERRWLGSFDASVL
                     ISEREASLVEDERLRQKVHVVGPSTPALVAADLPTVPKEVNLPREPVIGFVGAMDYGP
                     NIDGACWFAEKVWPLITSSRRDASWWLVGRSPARVLRRLDNGKNIQVTGTVAEVEPYL
                     EKMRICIAPLRIARGAQIKVLMAMTSGRPCVVTPGVAEGLGARPGRELLVGESPAEFA
                     EAVLRLLNDDSLAQDVARAGLAFVVGRLRPDVSLLRLERLLTSGRERGSACAFDGATA
                     SPCCQPGGLST"
BASE COUNT         5306 a         8115 c         7319 g         4383 t
ORIGIN      
        1 cctcgtgcat gtcccagtat gcctcgatgg aaggcagacc gagcgactgc ggcgagtcgc
       61 gcagccgatc gacgaggaaa accgcccctg ccaccgcgac caccgccgga accaggaagc
      121 acaaccgcca gtcgtgagtg accagcaggc tgcagagccc aagggccgcg gccgctccga
      181 tcgaatgcga cgtgttccac aaagcgaact tcgtgccccg ttcggcgggc gagaaccatt
      241 gcgtgaggct ccgcgcacag ggcggaaagc ccatgccctg aaaccacccg ttcatcaccc
      301 agaagacacc gaaggtcaga gccactccag tgaacccgca gaaaatgttc atcatcgccg
      361 agagcagcag ccccagagcc atgaaccatc gagggtttgt ccgatcaccc agaaatccgt
      421 tggcgaattt ggatacgccg tagaggatgc cgtgcagcgt caggaacagg ccgagatcgg
      481 ccttggtcgc gcccatcgct tcctgcatga ccggcatcgc gacgggcagg ttcttgcgca
      541 ccagataaaa gaccgcgtag ccaatcaggc tcgcgtagag aactctctgt cgccaataag
      601 cgtattggcg ctcgaccttg gcggcgtccc gaatgcgatc gatctgggga gcaggccgca
      661 acagtgttgt gatttgcctc agcatcaccc gatcccgcta ttcgtgtccc aattgctccc
      721 atgccccgcc tcgggcgaag agccgctcca agcattcagg cagaagcccc gtcagagtcg
      781 ccagatccag cacggtaaac ctacggcgta tttgctgtgc cgcaagatag ctgcgccgca
      841 accgcggcag gtcgatgccg attgcctccg gccggcacgg ggcccccgcc tgctgcaaca
      901 ttcggcacaa gacccgagaa ggaatcagtt gatcgcgcag tcgctctcgc aggtccggcc
      961 acacctcccg caatcgctgt agtagcgcca gcaactgttt tcgatcagga tgcttggcct
     1021 cgacttcccg gagcgaaaca tcccgcaact cgggcagcgt gtgcgtctgc cttacctgcc
     1081 ggcgaaccga ctccaggtcg ggttggcggt cgcaaatccg ctgcggatcc atcagttgaa
     1141 gcgggtatcg aagcaactcc tcgtataaca ttgtcaccgc cagcgttcct atgccgacct
     1201 tgtgcccatg cagcgggact gtgcctgcat gtcgatggtg ctccatatcc cagaggtggc
     1261 tgaactgatg ctcggcacct gaggccgggc ggctggaccg gcaatgctgc atcgcgaggc
     1321 cggtcatcag caggccctcc aacaaccctc ggatcgcgat acgattcccc ttttgaatcg
     1381 cctccgggtt catgagccac tggcgaaggc atctttgcac gagatcccac gccccaggct
     1441 cgatggcctc aatacccaat gcatcggcca gaatccaatc cgccccggcc gtggtcttcg
     1501 ccgcaagatc cgcgtagccg gccgcattca gctcgccggg cgcctcggat acgatgtcca
     1561 gatctgcgat cactgctcgc ggagctggac agaagaatgt ctccttcaag ccgtgtcggg
     1621 taatcgacgc tcccgacgcc gtgaatccgt ccatcgaggc ggcggtcgcc acaaccatgt
     1681 acggcctgcc gcagagatgg gccgccagct tgaccagatc gttgattgtg cccgaaccga
     1741 ccgcaatcgg caccgcaccg ccgccgttca ggaagctctg tagtcgctcc acgtgacgat
     1801 aatcggcgtg caaatccggg tcttcgaaga cgacgggctc cgttacaggc agaccggctt
     1861 gacgacacaa acccaacacg cggtgtccgg ccgcacgcaa tgtgttgcgg tcggcgacga
     1921 ctaccgctgc tgactcggga aactgccggc gaaagacctc ggccgtcaga gcaatcacgc
     1981 cttcgccaat ctgcacctcg cgcgtatccg aggccgtgcg gagcgcacgt tcgacccgct
     2041 cggcaaatcg ctcttcacaa gcaacctgtg ctcgctgctc gcagctcatt tcttgctccg
     2101 tttcttgtgg ttgcggacgg cctccagtgc cgcctccggg tcatccgtgg ccaacgactg
     2161 gacgccaagc tgcaacaatc gctcataccc ggccgcgtca cagccgcccg ggcagaagac
     2221 ctggaacaga atacggcgtg ctttgagttc ctctccgatt gctcgcagga acgcagcgga
     2281 cggctgaaag gtgtcctctg atgccaggtt acctaccttc acgtggatct gaagctgggt
     2341 cacaccgatg aaatccgccg ccttgacttc cttcagccgc cggctcagcc cggcctcgct
     2401 cccgcccatc cacagcagcg tctgtgaccg aggcagcaat tccttccacc gccgggtctc
     2461 ctcgtacttc gttgacgcca agatcacctg cgatgccacc cctcgctcag agaccatctc
     2521 agccaggcgc tcgagcggca cctccttgac gtcgagatac aaccatcgct cggggcggcc
     2581 ccttatggca tccagcacct ccaggatccg aggaatcggt tgtccttgcg gaccaattcg
     2641 caatttggac agctccgacc aggtcaggtc tctcgttccc ttgccttgga actcgggtgg
     2701 gagatcgctg accaaccgat cgagagtggc atcgtggaat gccacaacca cgccgtcctt
     2761 ggtcgtccgg acatcagctt ccggcactgt gccgagccgc cacgccagtt caaacgcctg
     2821 catggtgttc tcaggggcca gctttcctgc accgcgatgc gactggatga tgaactccgg
     2881 tgggcatgag cccgaagtct ggttgatgcc gtcgccaggc aatgaacacc cggtcgtcca
     2941 tgccaacaaa accacggccg aaaaacctcg cccgaccaga cgacgggccc caccacagaa
     3001 actggctgcc atctcgaagc aattcatggg tcgaccctca accggacacc aggcgcgcgc
     3061 aaaaaagccc ctgcgaaagc tcgaagctcg caggggcctt tctctttatt ctctggccct
     3121 caatgcgatt aacgctggcg tgaacccagt gttatgagta gtgtatcggc tggcggtgcc
     3181 cctgtcaaga atcggagcga ggccgctggg cccagaatcc gttcgccgct ggccccagaa
     3241 tccattctgg ggcctttcgg ttcgaggatt gccgttctca tcggcactga gcggaacgaa
     3301 actctgcaac gacagaaggc ccggaaagga ttccgggccg agcggcctcc tgtcaagagt
     3361 cagagcaaga ccatgaagaa gaacaaccgt tcagacgaca tccggcaatg gctgacacga
     3421 ccgatcatcc ccgtcgtctc ccaggccaat gccgacacgg ccctggtggc cgaggccagc
     3481 ctcctgttcc tccagggctg cgagctggcc gatctggccg acatcctcgg ctggctcaaa
     3541 aagggacctt tggcccactt gcccatcatg ctgcacatcg acttgctggc cggcctgagc
     3601 agtgacgacg ccggcctccg gtacctggcc ggcctcggcc agatcgacgg gatcatcacc
     3661 gtccgccccc acatggtggc cgcggcccga aagcaggatc tggcctccat cctgctgctg
     3721 ttcctccagg acggacgagc ggtcgatcgc ggcttgcaca tcgtcgagca atcagaaccc
     3781 gacctcatcg aactcgtccc cggcgtggcc gccctcgagg tcctcccgca attcgccaac
     3841 gtccccatgc cgcggatcgc cggcggcctc atccgcactg ccgacctggc ccgccgcctc
     3901 atagacgccg ggtgtgacgc agtgagcacg agtacggccg acctatggca actcaaccgc
     3961 tcaacgtctt gagaatgtgt acgagaaccg ccggctggtt cttggctcga accgtcgcag
     4021 ggtcagggtt tgctcgaagg gctctgcgac acagtgacgg cgtccggttt gccgttgcgg
     4081 cgcctattca tctccctctc tcagcatgcg aatgtataat acagcggggc cgcaagagcg
     4141 ggctgctcag gcgcgggact gcgcaccttt cgtgctcgat gagccaaggg cggcatgaaa
     4201 agttgctcat ccgcacgacc gcgagcgacc gttgtgcggc cgcgccgcag gattgtggtt
     4261 ctcctcgttc agggagcaag acacgatgac atgcgagcgt gcaagaatcg tcctgtgggc
     4321 cgtggcggcc gccatgctga ttgtcggccc ggtaacgggt caaatgaacg acggcgccga
     4381 agcagaaagc gagcaggcga agtcggctcc ggcggatgag acatccggcg ggcaggtgat
     4441 cgaaggtctg gtcctgaact actacggagg cggcatcacc ggagcacaaa tccgcgttga
     4501 ggccctgaat gccaagccgg gcgatccgcc gattgcggag ggcaagtccg gcaagggcgg
     4561 cgaaattcgc atccgcctgc ccaagcggat cgatgaaaac gtgctcgtcc gcatccgcat
     4621 ggacggttat caggagttcg tccaagaggt ggacccgacc gatcccgaga atccgccgtt
     4681 cgtggatgcc gcccttcagg gggccggaac gatcagcggc gtcgtgcggg ccaagtcgag
     4741 cggcaagccc gttgcgaacg cgaaggttct gggggcgagc ggcggccgtg acacctccgc
     4801 tcggaccgac gcgaacggca agtacacgct cacgggcatt gtacgcggaa gggcatacct
     4861 caaggtcatc gccgacggct tcgccacctt tcaaacgatc ctgaacatcg acagacaaga
     4921 ctctgtgcag gacgttgagc tggcggcgga acatcctgtc gagttgcttg tcatcaccgg
     4981 cgacggcgac ccggccgaca aggtgctgat cgagggctgg atcgaatcga ccaaaagctc
     5041 gatcgaaacc ataacagatg cccaaggccg ggcgacactg aggggcatcg gggagaacgt
     5101 cgaggaaatc cgtctgcgac tcaatggaga tcgctacgtc cggatgtccg gctttgacga
     5161 gcgaatcgat ctggccgctt cgcaacccgc gaccgaaacg aggccggcaa cgacccgacc
     5221 gagccgacac aaaatcatcg tgcggatcgg cgccagagtg cgaggaaaga tcaccaatga
     5281 caagaacgag ccgcttgcag gcgtgcgagt gaccgccgga cggaaaaacc tcgctgacat
     5341 gcccgtgacc tggaccgatc tggatgggga atacgaacta caagggctgc cggcgggact
     5401 catcaccatg accttccagc accccgacca tgcgccggac gtccaggaga tctcgctcaa
     5461 tgcaggccaa accggcacgc tcaatgccag gctgaccacc ggaggaaccg tcgccggcac
     5521 ggtggttggt ccggatgaca agcccatcga acaggcatgg gttgtcctgg aggaatggaa
     5581 aggataccaa accatcggca tgcgggccgt gaccgacgag caaggccggt tctcgttcaa
     5641 gagcgtcccg gaaggcgatc tgatcttcac gatcgtcaaa tccgggttcg gcggaccgat
     5701 acgccagacg atggctgcgg gcaagacgga ctaccggatt gtcctggaag agacaacacc
     5761 gccggcagtc acgggcggcc caggaccgca ggaggacaag attggtgtgg gccaggccgt
     5821 cccggatttc acgcttgtgg gagtcgatgg gacgaagtac aagctttcag agctcaaagg
     5881 caagtatgtc ttcctggatt tctgggcgag ctggtgcggg ccgtgtcaga gagaaatgcc
     5941 gcacatcaag gccctgcatg aggccatgaa aaacgaaccg agcttcattc tcatcggggt
     6001 gagcctggac ggcaatgaga aagacttcaa ggcggcggtc tcgaagagga acatcgactg
     6061 gccgcaggtg tggggacccg agtccggggc ggagaagacc tttgagatgc ttgagggctt
     6121 gggcattcct tacacttgtc tgatcggccc cgatgggcgg atgctggctc agcacatcgt
     6181 cggcccagaa accacggagc aggtcaagaa gcttatcagg aactgatcgc tggatcgcaa
     6241 gcggtcgaga cgcaggctcc accggcaggc atcctgctca gggtcggaac gggacaactg
     6301 aggggaacgt gatcagtcgc tcgccgggcc gggagaaccg caacacgttc caccgtacac
     6361 ttgccgacga catcgctatg acccggacgg ttgcgagacc gggcttgcgc caggcacgcc
     6421 cccgggaagt ccctgcggag cggtcgtccg gggcaccgcc ggacggggag gagccgaacg
     6481 cgtgcttggg cgagcagccg ccggcgaggt ggcggctcgg aatccggctg gacgggtgac
     6541 cggacgggcg gtcgcgggac gagttgccgg cctggatgcg gttccccctg ccgggcgggt
     6601 gctcgacctc gtcggttgac ttgccgtcgg ggcggcggga ggctcaggaa gcgccataat
     6661 cgaagccaga tccatcggct gaacaccgat aatcgggtac ttccgcttga cctcttccat
     6721 tgcctcggcc aatggtcggg attgcatcac ggcccgaatc tggaacttcg tgtgagggtc
     6781 atcaaagggg cgacgtccag gaacccgctc gagaacctgt atgatgtgat agccgatgcc
     6841 gctctccaca acgttggaga tctgtcccgg ctccaaagcc caggcggcgc ttatcagcac
     6901 ctcattcagc cgcatgtacc tgggcagcgt gcccaacagg ccaccctttg tacgagtgtt
     6961 gtcgttgctg agcttgacgg cttcatccca ggtcttcttg ccggacacga tttcctctcg
     7021 caatctctcg agagcctctc gcgtggcctt cttttgctcg ggggtggccg tcagaggcga
     7081 agcgaaaacg atgtgccgca atttgacccg tgtgtcgtcg tagtagtcgg ggtgctcatc
     7141 gtatatctgc cgaagatact cgtcgtcctt caccctcgcg gcaccgattt tcgacagctt
     7201 gccttgtccc agacgaatcc tgagctgcct gaggtactcg tcccaactga cgccggatga
     7261 ttgaaggatc atcttcatct cttcggtggt tttcgccttg gcgatcttga gatccttgtc
     7321 aatctcccgg tcaatatcct cctgcgtgac cagatccgga tgttcatcca gatagaccag
     7381 gaacagcttg ttggtcagtg ccgttccaag ggccttgtat gcggatcccc tgcgtctctc
     7441 gggaggcgta gtgcggagga tttcctccac gtcagcctgc gtgacgaccg ctttgtcgcc
     7501 gatgcgaatc agcactcgct cagaggctgg ttgactggct ggggttgtgg tcacgggtgg
     7561 tttctcaacc ccccccgcct gctccgagca gcctatcggg ggccacatca gcaaggcact
     7621 cagggcaaag actatgagct ttccactcgg cacggaacct gctgccatca cacatatcct
     7681 cccagcatgc ccggcggatt cagcgtcggg cgtggccggc tatggccccc ttgcggaggc
     7741 cgctcctatc tgcctatttg tcggtcactc ccggcggtgg ggtgagcctc cgattttggg
     7801 ctcggtcccg gccagaagcc gcccaatgtt agaccgatga cgataaacca ccaaggcggc
     7861 aatcacaata ccaaagacct ggagcggcca aaggaccgag aacccccccc atcggtcgcg
     7921 gttcaatatc gccagcaagg caaaaatgac cgggaaggca gtaacggcca caatcgagga
     7981 aagggagaca tagcggccga cccacaaaac aaccgcccac agcgtcaagg ccgccagacc
     8041 tgccagggtg tagtacgggt agattcccag ccccacgccc agcgaggtgg ctacgccctt
     8101 gccgcctttg aacttgagat agacaggaaa catatgtccc aggacgcagg ccaccgccgc
     8161 cagcacccat agtaaatacg gcaggtaacc cgggccggcc agccggctgt cggcagcggc
     8221 accaaaaagc agcaaccggc ccaaggttac aggcaggaaa cctttaagcc catcaagcac
     8281 aaagaccaag atcccataag gccgtcccaa gacccggccc acgttcgtgg ccccgatgtt
     8341 gcagctaccc ttggttcgaa cgtccacccc tttaagccga ccgactaaca agccgaacgg
     8401 aaccgccccg cagaggtaac tcagcagtat cagcccggcc acaggccacc atccactctc
     8461 aattacgtgc atcaacgcag gtctcccgca cgtccacgcc gccgctctcg tcacaatgta
     8521 ggcacgcacc tcagcggtcg gtcacgcgtg aattacgatc tcgcaacact tcctcgtaga
     8581 gcctcatcac ttcggtcgca tggcgggcca ctgtgaactg ctcaaccgca gccggcgccg
     8641 cctgggcaca gtgacgtcga tggtcggcgt ccgccaggcg ggcaattcga tcggccagcg
     8701 cggccaagtc cgtgggacaa tcgatcacat aaccctcgcg gccatccgtg atgcgctcag
     8761 ccgcgccatt gtacctggtg gtgatgaccg gcaaacccgc cgccatcgct tcgagcacca
     8821 ctcggctgca gggatcgtaa tacgtcggat gaaccaggac atcggccgca tgaaagaagg
     8881 cactcactcg ttgcgacgga ccggcaaaga aaacacgatc ggccagaccc gttgcggcgg
     8941 cccgctcgac caggttcttc ggattgtccc gccccacaat gatcgcgaat acattaccga
     9001 gctcgcgaac ccggccgtcc gccatcgcct ggatcatctt gtccacgccc ttaagtctaa
     9061 agttgtgggc gaccgccagc agaaccaggt cgtcgtcggc cagtccgaac tgccggcgga
     9121 tctcgatgcg gtgtctccgt cgctcgtgag ccggcgacgt atccggatcg acgccgttga
     9181 agatcagacg aatgcgtgcg gcatcgaagc cgtaatgccg tttgagctgg ttgctcacgt
     9241 actgcgagat ggcgatcacc catggtgccg ggcgacgggt caacaggcgt ttttccagtc
     9301 cggccagcag ccggtacttg agatccgcgc gctggcccac ccacttgagc ccgcgcttga
     9361 ccgccgaagg cctcagcgcc agattccgag acagcatttc cggaacggtg cccccacggg
     9421 gctggtatac gtcggcgaac ggacaagggg cgatggcatg aacgatatcg aattgatgcg
     9481 tgcgaaaata cttcgccgcc ccgcgcacaa accgccaggt tcgggccgcc cgccagcctg
     9541 cgccgacctt gatcgggacg atgctcatcg ccggcgtgga agggacatcg gtcatcgtta
     9601 caacggtgac gttgcagcct atgctcccga gatgacgcgc gaactgaacg gtcgaggtct
     9661 cagcgccgcc ccgccaggcc tcaattcgtt ctatgaccag cgccacgttc atcgacctgg
     9721 cccactgctt tcagccgcga cgactttgcg gcggcgacca agcatgttcc tcgtcttggc
     9781 gtgcaccttc gcggccagtc gccgggcaga gccaaaccgg gtgcaacacc ggacatatcg
     9841 ggccagaaaa cgcaatcgct cggtggtcgt cacacagtcg gccggcgtgg agtagtgcag
     9901 agccgccaga tccttcacca cccacctgca aagccgccat ctgggtcgga agacccgttg
     9961 gagatcgatc agccgatagc cctcacactg cgattcgccc ggcttgccga tctgcggctc
    10021 caggaaaacg tgtgacaggt aaagatcgcg atgaacgaaa ccggcccgat gaaactcggc
    10081 cacgaaccta gcaagacggt cgatccgttc gcggcggtgc tggtcgtcgg cacctgcact
    10141 cgtgcgagac aggtaccggg ggacccactt ttccagcgac gtcccttcca cagcccggag
    10201 tacgacgaaa ctccgcctct cccaagggcc catcatttcc tgaccaaaag cgacggcctc
    10261 tgcggcagga atgccagccg cagccagatc ctgagcattc cgccattcga tgccggccgt
    10321 gctgagacgc cagttaccct ccaaccaacg ggcacattgt cgtttgagcg gaggattcag
    10381 aaaacgcttc agataaagaa cttccgcggc agaggcttgg tgacccaggg cgatgcgcca
    10441 tcgctggcgc cagggttcca aaccaggttt gtccagtcgc tccccttggt gccatctaaa
    10501 gacggcatcc aagtcaccca gcccgcagcc ggacagcagg cgctcgcagc cgacagtcgt
    10561 ttgaaacacc gtattcatcc gtccgcgcaa agcaactgtt tctccaggga taaggctatc
    10621 ggccaccccc gcagcatgca accggacacc ccatctcatt accgaaatga cccggcagat
    10681 cgcggcgagc aaagttgaca tctgacgggc catcggcatc atgtggaaat ctgacggcat
    10741 cgtgcatgcg tagaacctga aggtccgtgc cggcgattcg gtcggccgtc tcctccggtt
    10801 tgcacgatgc acaaggctca aaccaggagg aaatgtcatg caatccggca aatccaatgg
    10861 accctcacgg cggcaggtaa tgtgcggcgc ggccgtggcg gcggcaacca cggctttccc
    10921 gtacgtgttg aagggagcgg cttttgacaa gcccatcaaa gtcggactga tcggctgtgg
    10981 cggccgtggc acgggcgcag cccacaacgc gatggaagcc gatcctgccg tgagaatcgt
    11041 ggcccttgcc gacgcctaca aggaccggct cgagggctgc cgcggggagt tgaagaaaag
    11101 gggagctgag atcgaggaca agcactgctt cgtcggtttc gatgcctaca agaagctgct
    11161 cgagctggac ctggactacg tgatcctggc cactccgccc tactaccggc cggaacattt
    11221 cacggcggcg atcaaggccg gaaaacacgt gttcaccgag aagcccgtcg ctgtggaccc
    11281 ggtgggtatt cggatcatgc tcgaagcggg gaagatggcg gacgagaaaa agctctcggt
    11341 caccgccggg acccagcgcc gtcaccagaa ctcctacatc gagacgatca agcgaatcca
    11401 cgacggcgcc atcggcaaga tcctgtccgc ccagtgctac tggaacatgg gccaactctg
    11461 gtacaaggag cgagaaccca agtggtctga catggagtgg atgcatcgag actgggtgaa
    11521 ctgggcttgg ctgtccggcg accacgtggt cgagcagcat gttcataatc tggacatcat
    11581 caattgggtg ctcaagacac atgcccgcaa ggccgtagcc atgggcggac gcgcccgccg
    11641 ggttaccggt gatcagtacg acttcttctc ggcggatctg tcgttcccga accccgaggc
    11701 tccggacaag gaaaacatgg atatccacgt gcacagcatg tgccgtcaga tcaccggttg
    11761 tgcctcgagc gtcagcgaac gggtcatcgg cgagaagggc atctccaact gcgccggctg
    11821 gatcagcaat gcgggcaaac tggacattcc gggcaacgag ccctacgtcc aggaacacgt
    11881 ggatctgatc aaggccatcc gcaccggcga gcagatcaac gagacgcaaa acgtcagcga
    11941 gtccaccatg acggcgatca tgatccgcat gtcggcctac accggcaagg aagttgcctg
    12001 ggacgatgca atcgaatccg acctcaagct cggcccgccg gactacaagc tcgaggaagc
    12061 ggagatccgc aagcacatcc cggtgcccgg gaagtagctt cgatgccggc caccgcgaga
    12121 ggcagggatc caggctttcg ttgacagaat cgcggagcag ggcgaccaag tgttaccctg
    12181 ctccgcctgt ttcggaacca aggccgctcc aaagaggaat ggcgaccacc tcaccctgaa
    12241 cctgcgcacc gcagtcaaac gccatgtccg gatggatccg gccagcggtc agcagccggt
    12301 gggctttatc ggacgttgcg aatctgcatt tcaattgcac aattgcaccg ccgggtcgac
    12361 ctgccgccgg aatgcattcg acgcactatc gtgccggtgc tcaggcaggc tgtgaccgga
    12421 tttccagccg tcatgctgac cggcccccag accatcaggc aagaccacgt tgctcagaca
    12481 cctgttcggc aagtcttaca gatacgtctc gcttgaatcg cccgacgtgc gaacggcagc
    12541 gattgctgac cctcgtggct ttctcgatct gaataccccc gcctattctc gatgaggtgc
    12601 agtatgtacc gcaattgttg ccctacaaca aggaacggat cgacgaacag cgccaccttc
    12661 acgggcacta cctgctcacg gcttcgcaga atctgctcac gatggagcag ataaccgaat
    12721 cgctctccgg ctatgagcta tgcgaccacc gcgacattga cgcacgcctc tgcttccggc
    12781 aaacctcaac cggcgccgag gtggacatcg tcgttgaaac ggaaggacga cttgttccca
    12841 ttgaagtgaa gctgttggcg actccacaac cagcgatggc ggctggtacc ttagcatttc
    12901 gagacgcata cggcgagggg gcggcacccg gatacgtcgt ccatcccgga aacatccgac
    12961 tgtccctggc acccggcgtg acggctatcc cgttcgccga gctgtgaaac caaaaagggc
    13021 cgcctttttg cgggccgcaa cccaagcgcg cttcaatccg gaaacatgcg cgcaaaatgc
    13081 acaccagttg cgacggaaga gactaaaggc acatgcccac gcaaaaccgt gggcatggca
    13141 cccggcatag gtgtccggct gttaggttcc aaaggctcac agcttctctc ccggacggag
    13201 gaacacgatc tcccacgcgg acttgcctcg gatgaaaaag cgggcaacgc gaccggccag
    13261 tttgtccgtc tccttccgcg aagcgtcgat accgatgact tgcggatacc accaattgtt
    13321 ctgcctgggg gcgaaccccc ggccgtcaaa gaccttcacc ggcgtggccc agccgttggg
    13381 atccgacaga tcagggcaga aaatcacgta gatgccctcc tgggtccact ccgtgtcgat
    13441 cgcccggttc atcaggataa cgtactggtc caagtacgcg ttgtagtgaa tggacggccc
    13501 ccagaagaca tcgaccttct cctggtgcca gtccgacctg gcgggaaaaa ccggcgtcac
    13561 gtgtccgccc agccccggct cacgccatgc acctttgcac catttgaaca acttaccgac
    13621 cggatgatcg cgatcggcga aggccatgcg tgcaatggca acaccctgct cggccacgtc
    13681 gcggttgtaa gtactgatga gaaagtagaa aaaccgcctc tgttgatcga ggatcaccga
    13741 gaagtcccca ctgccccctg cgaaataccg gttgggagtc tcacaataga gcgagtccgg
    13801 aggggcggtc aggatgatac caagatcttt ccagttgaga ccgttgtcga aagaaatcgc
    13861 cgccccgatc cggggagcgg tcagccgttt gtgcggtccg cagacttgag gcggttcatt
    13921 gtgataccaa ccgtatagca cgccgctctc gtgcttgaag gtggcttcga tccaccggcc
    13981 gccgatggac gggtcggcat cattatcgta gcgcgtgcga accgaaggcc ccttcagcga
    14041 gaaaagatca gccccctcac tgcgatcggc ataaccgacg gaggagaaca cgtacatggc
    14101 gtctccgtcc cagtgaacag gattgttaca gtcaaccagg ccggtcacat tcttctcggg
    14161 catggcggcg ccgggcagct ccatcggcgg tgccgaccgc aacacggcca cgggcacttc
    14221 acgccgcaca ttcggggtgg acggcttgac ggccgcacag cccgcaatcg acgcgagctg
    14281 gcacagaatc aggattccga agcctgtcct gattgtcata gctcgtcctc cgagactcga
    14341 tttcgccgga cgccctcgca taagccgttc gcattatgct gatgcgggtg gaacgctgtc
    14401 aatcgcaacc gttgtgaaat cggcccaatc cggtagactg tacggatctg ttattgagtt
    14461 ggattgacat gggtatcatt gccgacatac cggccatcgg gacgatatcg gaagaatcgg
    14521 gtcttattcc gctgccggcc accgcagacg gccgcttcca cgtcggacgg gacggggttg
    14581 tctcggtcac tgccaccggc gaccgcaaag tggagttcgt cgcgtttgcc ggtcacaccc
    14641 tggcacacgt gaagtccgcg atgggctacg cggcatatta cccggtacat ccggttcgac
    14701 tcgacccgcc cgtcgaggcg gtgttgatgg acctcgacgg caccagcgtg cgtagcgaga
    14761 acttctggat ctggatcatc gagatgacca cggccagcct gctggacaac ccgcggtttg
    14821 agctggaaga ggcggacttg ccgttcgtct cggggcacag cgtctcggag catctgcaat
    14881 actgcatccg taagtactgt ccggacaaga cggtggagga ggcccgccgc tactattttg
    14941 agcacaccca ccgcgagatg gccgcgattc ttgccggccg cggccgcgaa aacgccttca
    15001 cccccacgcc gggactcaag gactttctgc gcgagctcaa ggcaaccggc gtgaaggtgg
    15061 cgctcgtgtc gtcggggctt cacgagaaag cctggcccga aatcgtttcg gcgttccgca
    15121 ccatgaactt gggcgacccg aaagggtttt acgatgcgat catcaccgcg ggttacccgc
    15181 ttcgtcacgg agaggtcggc acgctcggcg agctgtcgcc caaaccgcat ccgtggctgt
    15241 atgccgaagc ctcccgagtc gggctcggca ttcccttcga gcgacggcat cacgtgatcg
    15301 ggattgaaga cagcggcgcg ggcgtctgcg ccatccgcct ggcgggcttt gccgccatcg
    15361 gcctggccgg aggcaacatc gcagagagcg gaacgaagac gttgtgcatg agttattgtg
    15421 agacgtttga ggagatagcg agttatctga ggtagttgtc agtcgtcagt gatcagtttc
    15481 agccgaaatc cgccgggcaa tacgacgaat aaaagcggct caaaaagccc gtccgactga
    15541 aaactgacaa ctgaagactg agaacttctg atgctcccag ggagcccccc atgcctgtca
    15601 acacgatcac actcgacggc gactggttgc tgaaggattt cgatcatgaa cgaggcgagg
    15661 ccgcgcgtcc gtttgatgcc gcgtgcccga ctgacgactg gttgaccacg ccggtgccgg
    15721 gcgacattca cccgacattg gtcgaagcgg gccggctgcc gtcggacatg ttcatgggca
    15781 cgaacgtcga gcaatgtcgc tggacgggtg aacgcgaatg gtggttccga cgggactttg
    15841 acgtaccggc cgatttcgtg ttgcagcgat ccgagctggt attcgagggc atcgacctgt
    15901 tcggcacggt gtggctcaac gggcacatgg tcggccagac ccgcaacagc caccgcgcct
    15961 atcgcatgga cgtcaccgac gtgatccggc ccgggcagcg caacacgctg gtcgttcgca
    16021 taggggccac gctgcgaatc ctggaggcca acccgtacca gaagtacttc gcctgcttca
    16081 acacgcctcg cgttttcgcc cgcaaggctc agtgccagtt cagttgggac tgggcccccc
    16141 atcttcccgc cctgggaatc tggcgaagcg tgcggcttga gtcgttcgac agcggccggg
    16201 tgatggacgt ggccgtccgg gcgagaaacg acggccatgt cacctttcac atgaccctcg
    16261 atgagagaac ctgtcggcag gatcttgacc agagcagtac cgaggacaga gcggcggaaa
    16321 ccgtcaagcc aacgggcaac ctgttggtcg agatcaccgg accgttcaac ctggccaagc
    16381 cggtcaaggc cagaccgatc cggcacggaa tccccatcac cggccagaag aatttctgca
    16441 cgctctgtgt gccgaaaccc cagctctggt ggcccaacgg ctacgggcaa caaccgctgt
    16501 acaagtaccg catcacacta atccgcaacg gccgcgcgca tcaccaggtc gagggtcggt
    16561 tcgcgttccg cgaggtcagg ctcgttgagg agccgctcgg cagcgatcgt ctgagctttc
    16621 gcttcgagat caacgacacg ccgatcttct gcaaaggggc caactgggtg ccgccttcct
    16681 gtttccccgg cacgctgagg ccggaccgat atcggcatct ggtccacctc gctcaggatg
    16741 cccacttcaa catgctccgc gtttggggcg gcgggatcta cgaatccgac gctttctatg
    16801 acacctgcga cgaactgggc atcatggtct ggcaggactt catgtttgcc tgcagcgact
    16861 acccggacga tcatccggca ttcatcgacg aggtcattcc ggaaatcgaa taccaggtca
    16921 agcgccttcg caaccggccg tgtgtggtgt attggtgcgg cgggaacgag aaaaccggct
    16981 cggccgggtt caaggtcagc tacggcgagc ggctgttcca cgtcatcatc cggggcgtat
    17041 gcaccgacct cgaccccacc cgcccctatc gaccggccag tccccagagt tacaccgacc
    17101 tgggcaatga taggaactcg ggcgactcgc acggcggctg ctacgaggat gcctaccagg
    17161 caggcatcac ctgctggcgg gagaaactgc gggactacaa cacggtgttc aattccgagt
    17221 tcggctacca cggcccgacg cgaatgcagt cgctgatcaa gttcgttccc cgggagaaga
    17281 tctggccgat cagcgaggaa atggaatacc acgtccagga caacccgtac aacaccatcc
    17341 ccgagacgtt cgcccaggtg caggccaaga tggcctcgac gctggtcggc gagatcagcg
    17401 acgcggaagg cttcgtccgc tgcgcgtcga ccttccacgc cgagctgatc cgcgcggaga
    17461 ttgaacacca tcgccgccgc aagtgggaaa acgccggagc gatgttctgg atgttcaacg
    17521 attgctggcc gtgcgcaagc tggtccgtgg tcgattacta cctggtaccc aagccggctt
    17581 actatgccgg gaaacgcggt ttcgcgccta tcatcgccgc gatcatcgac gagccggatc
    17641 actatgccgt gtatgtggtc aacgacacgc tcaagccgct ggccgggacg ctgaccttcg
    17701 gccagggccg cgttgccggc aaacccgtct ggtcgcgcaa gtgccgcgtg agagtcggcg
    17761 cgaattcggc acagctcgcg gcatcgatcc gcaagaagca ggtcaaggcc acgcccgact
    17821 cctatctgtt cgcgtcgttc ggaaccggag ccagatcgct ggccgagacg tcgttcttcc
    17881 atcggccgtg gcgggagatc cactggccgg tgcccgagct gagctggcga accagcgaga
    17941 gcatccgaaa acccgccccg ctttcataca agcccggccc gcactacgtc atgagaatca
    18001 acttcagcac cgtcaactac gcccgcatgg tgcatatcga cggcatcgaa agcgagggcg
    18061 tcctgctcag cgacaacttc tttgacatgt tgccgggatc aacgaaaacg gtcgaaatcc
    18121 gcagcgacca ccgcatcgac ccgacggcca tgcggatacg tcattggctg gatcggtggg
    18181 aagaatagct ttcagcaatc agcagtcagc ctcttgacga gccacggggt ttatccccgt
    18241 ggtattatga tgtggtcgcc atcgattctc actgctctgt tatggaagct attagcccaa
    18301 gttgaacact tgtgagccaa aaatggcctt agcgatagcg ccagggccgt ttatgttcga
    18361 aagtcgtgac tacatttcgc ggtcctctgc gagttccgaa gcatccgctg gtcgtggtca
    18421 gcgacacatc cagttcccac tgtaagcatg tatcccttca cgatgttagc aagcactaag
    18481 gtcgtgacta cacggccttg gcggagcaaa aaccgcatcc acgacctcgc cagtgcggtt
    18541 cgacaccccc caaaaaacct ggaatcgcct ttggctgttc aacttgggtt agcgcacgac
    18601 cgaaaaagaa gcaccccgcg catcgtcgat cgctgcaccg ccttcacggc tttgaagatc
    18661 gtcaatggtg ttaaccggcg atttcagggt agatacgcat ctaccggttc aatgatccgc
    18721 accggccacc ccaagccgcc acatcaccat gcttcaacat caccaggagc aatccccgcg
    18781 gtgctggtgc gtcgacggca cggccgtggt cgtgccggcg gttccggccg ggagaagatg
    18841 ggtcagacgc atggccgttc gagggttgaa ccggaaaccc ttgagcatcg cctcttgacc
    18901 atcaacgacc ttaaccggct caaacgcctc attcacgtcg atgtcgagca acaacgcggt
    18961 ctcacggccc ggagcaatca tgaacccgca atccagagtg acttgcgccg ccttcctgcg
    19021 gttcccgtcc ggaacaaggg tgtcatccgg agtctcgtgg tcaggccggt cgagggtgac
    19081 tcggccttcc ctgcacgtga gacggagact cgtgtacgga ccttcaggaa gatcggcgtt
    19141 gataagcagg ttggcttggc ctgcctgaat ctcgagtaag tcgatgattt gctcgccttc
    19201 ctgaaccacg atccacgact gatcggcggc gaccgctgcc gcggcctgcg gcaagcccag
    19261 aaaacccgcg ttttcagcaa gctgcggagc cgtgtgttgt cgaagcacct ccactcgcgt
    19321 gacggtcaag cttgcccttt tgagcggctc cgctggcgcg ggtgtcctgc ccatgaccac
    19381 cttcagcctg cctgtgggcg gcgcaggcgc cccggccgag ccccacgggc ccaagagccc
    19441 cttgactgaa gcacccacca tgcctgccac gatcaccagg cagacgagca cccctatacc
    19501 cgtttggcga tgacggcaat ccatggcccc ctcacgtctt gtggcttacc ctcaatcccg
    19561 aagccccgtt ccacgagtgg ttcgttcaca acactgattc acgcattgac gcatgtcccg
    19621 ccgattcccc gagcggaaaa caatgccaga ctgtttatcg tctaagcccc cggcgtgacg
    19681 ttagcgaact tgaggttatc ggctgagggg cgggctcttc gggttgatgc ctggtccgcg
    19741 ggccctgcca aaacacatca acaaggcccg agaaacaagg gagaaaagca gttatcaacc
    19801 acgctgattg gaaaaaccgg cctcgctggc cggggtcaga aatcgcggcg agcctgacgc
    19861 ctccagccgg ccttcagctc ggaagttggc aaagcggcgg atttccagaa aacgtggccg
    19921 caccggatgc actgcacgcg acccaatctc gactcgatcc gaacattgac cagcccgcac
    19981 tccgggcact ccgctctcac gatttccgat cccatggcac caaagttgaa catatcgcaa
    20041 ctaccaatga gaggaacgct tcttctccaa agcctccggt cagcaagccg tgcggctcgt
    20101 gcctcgcgcc agaaaccaga gatcgcccct ctcctccggg cacccccgac aacccccggc
    20161 ccgacagacc tttgcgagct atccaatgtt atggtcgtcc atctggctaa ggcaaaggcg
    20221 gaaaacaccc ccatccaggt ctcataaacc ggatccgccg ccgtgaatcg caatgactcc
    20281 aagcgcctga tcttgaagtg ccccgcatac gcgcataccg atggccaaac ggtatcttcc
    20341 cgctcaatct atacacgcgg gatccgctgc gatatccgtg cccctcaagc atcaccgaga
    20401 gaggccccgg tcagcttggt cgatctgctg attaccatca cggcatcctc gcaggcaaag
    20461 ccttggactg cgccgcacca ccgatgcagc ccacagcccg atggatatca tcgatgtcaa
    20521 catcggcgcc tccgtcgaac gcgtcgcgag ccgacaggat cacatcacct gcggttgcgt
    20581 cctccgtatc gaggatcctg cgcggcaaac ccttgatatg aattgtcact cacaggaatc
    20641 tctgccacaa cccaaagagc acccggccta cctgggccga aacgatcagg cggggcaaag
    20701 ccatcaaatt gcaggatggg caacggacga tttcctgttc gagggtcagg gcacaatgat
    20761 agcccatctc gcgaataagg acttcagtct gagacgacat tgcagccagg cgcccgtagg
    20821 gataggaaaa gtgcgggctg tgatcgccga tccgctcctg gagcaagtcg cgagcagcgg
    20881 ccagttcacg tcgaatgtag tcggacgtct gcggggccag agcgatatgt gacaggccgt
    20941 gactgccgac gtcgaccccg gcaagcttca attcacgcac ctgttgccag gtcatcgggg
    21001 cgaaagaagg atactgctcg cgcagaaaag cgatctcatc atccgagtac agtgagagca
    21061 attcatcgaa acgagaacga cgctggggct cgggcagata gcggcaggcc tcgatgatct
    21121 gcttgaccgc ggcacttcga tgctcgggcg tgctcaggtc aaactcgacg gcctggccgt
    21181 cccagtgcag cacgattcgt ttccgcccgc cgcgatgaat cagcacgcgc acgtcgtcca
    21241 gccactgtcg cccccccgtc tcgataagac cggcaggcag aaagaccgac catggcatcc
    21301 gtctcaaggc cagctcaccg gccgcaagct cgtggcagca ggcataacca tcgtcgaacg
    21361 tcaccacgat gctcttggga ggcggggagc gacgctctct aatgcaggcc accaactcgg
    21421 tcaggctgat cacgtgaaac gaacgatgga gcacgttcag caaccggcgg aaattgcgca
    21481 gactcagaac gttgtcctcc acctggggat ccggacgttc ggtaataccg tgaaagcaca
    21541 ggattcccgc ctgctgttcg atgtccggga ggtggttatg ggccccagcc aacgccatca
    21601 tacggccggc gcagcctcgg aggcggtcgc cgcttgcggc aggcgacgcg gcggcccgcg
    21661 cctcgagccg ccatgacagg ccctcaaaca gtctccgacg gatcggcatt actcgtctcc
    21721 acttcccgca cgcggcaaac cggcacgcaa aagtcacaca cgctcggcaa aacggccttc
    21781 cagcgcccgg tacaccgccc atccgcttgc tgcgagcaca acagaaacgc cgagggcggt
    21841 taggaagggc acggcctccg gcaatcgtcc atggatcacg atgtcacgat aggcggagat
    21901 gatcggaacc atcgggttaa ccgtcgttag ccatctgagt gccgccgaac cgtcagtcgg
    21961 cagcgggtac aacacattgg tcagaaacat ccacatctgg atgccgaccg cgaacaggta
    22021 cttgacgtcg cgataccaca gattgcccag ggccagccaa agacccaggc ccaccatcag
    22081 aaccacctgg ataaccacca cgagcggcaa catcagaagt accgaacgaa acgcgaatga
    22141 ccagtcgctg gtcgcgtcga agtaaaccac caaacccacc agcacgacgg aggcgacgag
    22201 aaagtcaaag aaagcgctgg caatcgatgt cagcggaaga atctgctgcg ggaaataaac
    22261 cttggtcacc aagtcccgtt gcgacgtcaa gctctgaatt gagccgttca ggcccgtcgc
    22321 aaaaaaggtc cagggcaaca ccccgatgta gacgaacagc gggtagggca cgtccaggga
    22381 ggcgatcgcg ttgagcgaaa ccgcgcgcgt gaagatgaac gtgaacaaca acatcatgcc
    22441 cagtggctgg agcagtgccc aggatacgcc cagcagcgag tggcgatagc gcaccaccac
    22501 ctcgcgccgc acgagttggt acaagagatc gcgatagagc cacagttgaa gcataaggaa
    22561 acgcctagcc gtcagctatc aatccgttcc tcgtatggcg tccgcggccg gtgaaaccgt
    22621 caggcccctc aaccaggcag ctcgctcgcc ctgacgctgc ctccgccaaa gccgggaacc
    22681 cgctaagtcg acgtcatgac cgcggcttcg caacgctcct cttcagcaag tgcctctgcg
    22741 aggcccccgg agcgcccgca gggcggtgtc ctgccgcacg cgtcgataat ggcggcgatc
    22801 tgttcgagcc gggcctccca ggattcgctt ctcatcaaat ccgacctgcg ccggcggtcg
    22861 gcggcgctgt tgcaggcaag cgcatcctcg cagcacctta tcattcgctc cacgtcgtcg
    22921 gccacccata cggcgttggg tatgggtcga accgagggca acccggtgct gaccaccggg
    22981 acgccggccg ccagatactc gtacaacttg atcggattgg cgcttcgcgt caggggaacg
    23041 tgcttgaagg gaatcagtcc gatgtcaaac aggcgaagat aggccggcaa caaaacgtga
    23101 tctctttctc cgagccaata cacgttcggc agatcctcgc acgggccgtg tggaacgagc
    23161 accggtccga tcacgacgat cgacgcgcgc cgatattcgc gcgccagagc ggccagcaga
    23221 tcgagatcga cccattcacc cacaaccccg atgtaaccga tgatcggtcg ccgcagtgat
    23281 tccagctccc tcggggcatc cagctcttct ttcaccgccc ggctcaactg ctcgtagtcc
    23341 acgccgtgct ccacaagatg cacgttgcgg tgaaaccgtg acttgctctc atagagcggc
    23401 cgggaagtcg cgatcactac gtccacccgg tccagcaact gccgctcgcg attctcgata
    23461 agagcccggt cgtagccggg aaactcgccg aaagcgtcca cacagtaata cagggctagt
    23521 tcctcgccga aacaacccac gaagtcgctg acgtccgggg cgaagctcca gagctgcacc
    23581 ggccgattcg gtaaggtttc gaccacttgc tcgatcgccc gtcgcacggt gaatctgttg
    23641 aaccgacgtg ccagacgcat cgaaggcatg ggcacttgga agggcgtcac caccgcgatg
    23701 ttgtgggcga ctcgctgggc accttgacgc atctgccgga gcttgtccca aatcatccga
    23761 aggtcgtacg gatgcatcgt cggccggcgc gaagcatgcc aattcaccca gacaacgttg
    23821 ttgcgtctgg ccaggcggcg catgatatgg tgcttgctgg tcggatgaaa attccagccg
    23881 ctcgcgaagc aaatgatgtt cttgccgctg atcacgtcga tagacccccg ggctgacaac
    23941 aaggcgaggc tgttgccccg tcaaacgcac aggccgatcc tcgctctctg cccgatgtca
    24001 gaagccgctc caggcgaaga agactgacat ccggtcgcag gcggccgacg acaaacgcca
    24061 gacctgcccg ggccacatcc tgcgccaggg agtcgtcgtt gagcagtctc agcaccgctt
    24121 cggcgaactc ggccggcgac tcgccaacca gcaattcccg ccccggccgg gctcctagtc
    24181 cctcggccac accgggtgtc accacgcacg gccgcccgga ggtcatggcc atcaacacct
    24241 tgatctgcgc cccccgcgcg attcgcaatg gggcaatgca aatccgcatc ttctccaaat
    24301 acggctccac ctcggcaacg gtcccggtca cctgtatgtt cttcccgttg tccagccgcc
    24361 gaaggactcg cgccggagat ctccccacca gccaccagct tgcatcacga cggctgctgg
    24421 tgatcaacgg ccagaccttc tcggcaaacc aacaggcacc gtcgatattc ggtccgtagt
    24481 ccatcgcccc cacaaacccg atcaccggct cacgcggaag attcacctct ttcggtactg
    24541 tcggcagatc cgcggccaca agggccggag tactcggtcc caccacgtgc accttctgac
    24601 gaaggcgttc atcctcaacc agcgacgctt cccgctcgct gatcaggacg ctggcatcaa
    24661 agctgcccag ccacctccgt tcgcggcgat caagccggcg agcctcaatc cggtaaaccc
    24721 aactcatcgg ccaggccgca ccggccgcag actcagccca tttgcggctg tccagatcgt
    24781 ccagattcaa cacccgacgc gacgccggaa cctgaagcgc cagcggggcc atgctcgacg
    24841 aaaatgccag taccgcgtcg aaacctacct gctcggccca ctgcatcacc tgccgccgca
    24901 aacgccctga ggcaaagtat tgctccgttg ccgtcccgcc ggccaacaac cccgtcaacg
    24961 cacgagcctg tgcaagggac tcatgtagcg gcaccgccgc gacctcgcgg cacatcgacc
    25021 gcagtgcccc cagcgatcgg cgggcctcct gcgatgtacg ccccaggaag cccgtctcga
    25081 gaaacccggc gcaccagacg tcgtgccgct gcgcaagaaa ccg
//
LOCUS       DAOTPW010000055        25102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2065_length_25102_cov_5.663393, whole genome shotgun sequence.
ACCESSION   DAOTPW010000055 DAOTPW010000000
VERSION     DAOTPW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 25102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25102
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_25102_cov_5.663393"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1196
                     /locus_tag="P5316_06960"
     CDS             438..1196
                     /locus_tag="P5316_06960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR84734.1"
                     /translation="MDLGMDMWKYYGITHTDHTVMNPLSLEKTRELVGLLRLPNGGHI
                     LDVACGKAEFLCLVAEAYEVKATGIELSPYTIKAARKNVETRGLADRIELLHTDGGNY
                     EPNAPESLDLASCIGASWVYENHRGTLQALTKMTRSGGLVLVGEPFWMTDPDPEYLKL
                     TGVDPNLWGSHAGNAAIGEDVGLALLYTLVANPDDWDRYEGLQWRAAERYAVDHPEDP
                     DVEALLRSTRRSRDAYLRWGRNCLGWAMYLFQKP"
     gene            complement(1957..4077)
                     /locus_tag="P5316_06965"
     CDS             complement(1957..4077)
                     /locus_tag="P5316_06965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRR84735.1"
                     /translation="MSEPWLEGLNEQQREAVKHGDGPLLIIAGAGTGKTRTLASRVAE
                     LLHRGVSPERILLLTFTRRAALEMISRADQQTGLKVSSRVWGGTFHAVSNRLLRIYGR
                     ALGLPDGFTVMDQGDAADMMSLIRNELGAAKEKKRFPNKNTIVAIYSRTVNSQDKLVD
                     VLDLHFPWCREFEKELIAIFDLYVDRKRANNLMDYDDLLLHWKLMCDVPGVGERVAGR
                     FDHILVDEYQDTNVVQADILVGMRRRNNNICVVGDDAQSIYSFRAATIRNILDFPQRF
                     PGTRIIALEQNYRSIQPILDLSNAVMEPARERYTKNLWSTRTAERRPVLYYCMDEDEQ
                     SRLVCERILEHLEQGVPLRQQAVLFRAGHHSDALEVELMQRRIPFRKFGGLKFIESAH
                     IKDMLAFLRVLENPHDEISWFRILCMMRGMGPQSARRVMQQLGVRRQSPAQGDEQEAS
                     GEMGSPLSRFWSYNLKVPPDSKKSIADLREALKACYGVVNVDEESDEAQSIVATGSAP
                     TLAVQIQRLREYYEPIFKTIYDNWQIRIRDIEQLEQIAGRFRSRRRFVADLTLDPPNS
                     TADLAGPPCLDDDYLILSTMHSAKGCEWTSVHIIHAADGMIPSDMATRDESQIDEERR
                     LFYVALTRAKDWLYIYFPLRYYRTRYPAGDAHSIAQLTRFLPKKALALLEKRSDSPPG
                     FVDDELPPGGRSHIDAQLKKRWRS"
     gene            4256..4480
                     /locus_tag="P5316_06970"
     CDS             4256..4480
                     /locus_tag="P5316_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84736.1"
                     /translation="MTYRGKVKDGVVVLDGDVKLPDGATVEVSLPERSANEEAPTLLE
                     RLGPVIGKAKGLPPDASQNIDRDLYGQGKR"
     gene            4477..4878
                     /locus_tag="P5316_06975"
     CDS             4477..4878
                     /locus_tag="P5316_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRR84737.1"
                     /translation="MNPRFADTAFYVALVNPHDELHVAAIELARDFRGTITTTEYVLV
                     ELGNRLARSGDKEVFIELVGQLQADARTIVVPGDPSLFRRGLDLFADRLDKERSLTDC
                     TSFVVMSEQGITDALTGDHHFEQAGFVALLR"
     gene            5076..7730
                     /locus_tag="P5316_06980"
     CDS             5076..7730
                     /locus_tag="P5316_06980"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HRR84738.1"
                     /translation="MIQRCVRITPALLSATASAFLATTGTSGAASPEPAAAANTARWI
                     WSDPADPAPKNRFTWFRKVVELNDMPADATLHLAADSNARVWINGQIVRRKVARYHED
                     RITTERINAGPYLRPGPNVILVLHHNWGDIITFQRTGNKHAGLYVSSTWVNSDASWKC
                     LKAAEYMEHEKQIPGVIGDKRIRYPVIADGSKVIAGNLHDPMFDDGTWKPAVVIENGP
                     WPARPSNVETPGQREYPVRPAKILAAGTAERTMLISDDPASMAAGIRTSKCRPETELT
                     GIAGDLISGKPITISGQAGETKYITFDFHQPIHGYPFIGLKDAPAGVKIDLGYCELSR
                     SLCTGKLHVDETGWINPEGVVGPGYADRYITRAGTQSFEFPDERTARWLSLHIHFAAD
                     GQVILSDVGITKSQYPITPVGSFQCGNELMHQIVKLCLIHAEVTMSDTYVDTPGREDG
                     QWIEDNRPRAILAARWFGDNRLRRFMIRTFAQGQGKDGQFHPFAPSNYPAYPATYDWG
                     VEWTAALYDDYMWTGQTDLVREYWDTLRRFWDNCLTHVGDDGLWRTPHIFADIRNSLR
                     PANDKQSSGIVTPWIIQRLGWSVEMAEAVGKTEQAAKWRETREKMIVAFGKYHIVPPK
                     GDVPPHVGDRVDPADPNLDRGYCQAGQTIAIIAGLLDYSEAIADLNYAFQDPDGSPGP
                     GVKRWNNPTYGYRSLRALSDTGFTQRAVNHVIERYAPYLPGHPRNQVPPVLQGPYGGP
                     LPEYWISREDLGLKDGEKNPTQPDDETGSHGWGALPLLWLHESLLGVRIVSPGGNKIR
                     ITPEDGGLPFVAGHTCTPKGVVWVYWDPQQWRLEVSIPADVEADLVLPKQCRGKRIAI
                     AESAGPVEPAGEERYTLSAAGRCVLIAR"
     gene            8124..11615
                     /locus_tag="P5316_06985"
     CDS             8124..11615
                     /locus_tag="P5316_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRR84739.1"
                     /translation="MPVRFVVGRSGSGKTWRCLEAVRARLREDPVGGSKLLLLVPEQA
                     SFQMERALIETPDIHGFIRCEVLSFQRLAYRIFAETGADPRRADETIGPLGRMMAIRR
                     LIRREKNNLRLLGRVADKPGLVRQVSSALDELMRADLEPATLAELAERYEGANPLAAA
                     KLADLTRLYRAYLDYLEDGRLDPAQYLHLAAERLDACTWLNGAEVWVDGFAGFTQQEY
                     VLLVKLAQRAAFMEIALLVDPRASAIEAIELPKTTFSLFARTERTLVRLRRDLTTAGV
                     PIAEPVRLDPWPPPRFVAPELAALEKHLFRTGSPEDTGAAPTAISLLEFPDRRCEVDA
                     AVAEIRRLTQLPDAPLRYRDIAVVVRDLTEYHDLLSASLRAAGVPFFIDRRQPTTNHP
                     LIELVRGLLSVASDDCRLESVRLTLKTGLLPIGDDDADTLENYLLAHGIAGRDAWREP
                     WTFTRMFRRRGRKAGLSDAQKSILNRVNQTRQAWLDAVGPWLETAAAPEGHRGRTWAS
                     SLFACLERIGAGGHLERWARDAELDGRSDEADHHRQVWVDFVRFLDEFVMALGGDRMS
                     INEFRETVEAGLAEFDLGLAPPTLDQVLVGAIERSRHPPVRAVLLLGFDEQHFPMRRT
                     EDPLLGDLERDDLDRAGMEIGPSRQRQLLDERMLAYIAMTRAGERLWISYPRAAGDGT
                     PVEPSAYLPDLLAAVPGLRPQRCDDPRLTRTLAGISRVPELAERLAQELRWRPRAPDE
                     VDPAARARWNAIYEAARQHVTWQHTLRMCLSGLAYRNQAKLEPGLMEQAVPWPFAASV
                     SRLERFAACPFAHFAEHTLALEQRMEADLGTLDLGNISHAVLEMFIAELAREGRRLGD
                     LDDDEVIDRIDRTAAELAPKLGGDMMLAEARNAYLFDRSRNHLHRVTQWQRDAARSGR
                     YYPVRTEREFGYRENEKVVITTPRGRRVELHGIIDRVDVVELADCYGAVVIDYKTVSE
                     RRLKMAEVYHGLTLQLVGYLLALQQAGESLTGRKVRPVAAFYLPLLQGFETVSHPSKA
                     TSPTEAYRFRGILDLTALDGLDTSAASDRKSRYMSAGLTKDGKPHQNSDLASPKEFEA
                     IMAHVSRKMGELADSLLDGNVEVKPYRLNKTSPCKWCPYMAVCRYEADWQPPNTLAPL
                     GRRADILKRLTEEHPDA"
     gene            11608..15447
                     /gene="addA"
                     /locus_tag="P5316_06990"
     CDS             11608..15447
                     /gene="addA"
                     /locus_tag="P5316_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HRR84740.1"
                     /translation="MPDAAPRDRYAHLTERQREAIQTTGRSLLVSAAAGAGKTTVLAE
                     RCAYLICDLPKADRCGIDELLVVTFTDAAAGEMRTRIRKAIRQRRQESSHDPWLDQQI
                     HLLETASISTIHSFCKTVIQQWFPQAGVDPQAAVLAEDEADLLRHETIDALFVELYGR
                     NDEYGRDFQSLVDDYGSGDDARIAEALLAIHAFIASLPEPQAWLDRAVQVLEPGAPQS
                     LADRIDEIQHRRLIRELELHVRQARESARVIDSLYPVASMHADALREYVSQCEAWLDR
                     LNAGSQDAWGEIAREVAGHTIATERRPSRLPEAQVAQFDAAKKIVEWHRDLLEKRLQD
                     GICSFSKNEYIEGLQRILPYVRTIVRLVKDFGDRYQQAKAVQAAVDFNDLQRQAYWLL
                     SRDGDPNRPSEVALQLQKRYRHVLVDEFQDVDPLQEAILRLVSRETAEPPEGNLFAVG
                     DVKQSIYRFRLAEPALFTRRADAFSSESTIGRLIPLGDNFRSREGIIDAVNLVFAPLM
                     SRSFGGSDYDETARLRAGACYPPSEGLRVFDGPAVELHLLEPVRGMAQAVDAGDEGTG
                     DPGATQGDSQQDELAGIEREAFLIGRRIRDWMGSSGARMNVADRPATPGGPPVLRPIE
                     YRDIVILLRSMPHKAEPIANILRRMGIPVLIGRGESPADSTEFRDVLSLLRVLDNRQQ
                     DIPLAAVLRSPLVGRPLTETQMLRVRLHDGEVPFHRAVMRYAENGPDEELRYRLSVIL
                     ATLDRWRTRIRRLPVAEVLWDILEETHYPAFVAGLPDGARRRERLIQIHNLARDFGQF
                     RRQGLRRFLRFMDELIAADRSPQPAAGSGEDDNAVRIMTVHTSKGLEFPVVIVADLSK
                     SFNLSDVKESVLVHRNLGIALRAADPERRIHYPTLIHQLAVEDDRRESLSEELRVLYV
                     AVTRAREHLVLVGRIQPERLAVFRMPREPSAEKLPRLQLEVANQPLTWLLAAIGAAPA
                     DNVRWPEDAGASGSILIEVHQYPRAETDHWRTPPAVAPERLDALANCARLGPLPGDEP
                     VQGSDEAPRLIASLDAPYPALELTTLPARVAVTELKRRWDAWSDPDLRPGKPPVAGPA
                     PAVPVFIEPLPVAQAVNRGMATHRFCQLIDLARPCDAADLAAQHRFLREAGRLSEAEA
                     SAVLLDDVAWFFSTALGHHIRKHAGRVRREIAFVSRIIPDRYEPMVTARDSRDVILVR
                     GTVDVLLCHEDHLEIVDYKTDAIPHEECTERSRLYQEQLDNYAKALHGIFQRPVVGRW
                     LVFLQGRCVVALAAPTA"
     gene            15383..18007
                     /locus_tag="P5316_06995"
     CDS             15383..18007
                     /locus_tag="P5316_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRR84741.1"
                     /translation="MADGWYSCKAGASLPSLLQQPDLLPTSRLSHLPVSRRAELAVWL
                     NDEPLVLPAAALAAGIWLDAVWPVPGWLSMLLFLAAGGLLLIARRRPWFVYAVLPLAA
                     LSTGAAVHDLHFRRQPTNHVAHYCARESLRVRMTGTVLAPPQIRSAGTGRITWFKELP
                     RTCLLVEAEHLERRSGPLTVCGMIAVHVRQPVLHITAGDRVEIIGTLYQPPPPDNPGE
                     KDWRLILRRKGILAEMSCERDADLVVKGVSDPGRRWIAGLRRRLKAAMIDQAMPGDMP
                     GAKMLAALVLGQRSAVDDRLNQAFVNTGTVHYLSVSGAHVAMLASVVWMIGTVAGVSR
                     RSCALWAMMLIIGYALLTEPEPPVVRSALMGTLLCISVLMRRPVRSANWLALSAIILL
                     IIAPLQLFDPAFQLSYLTVPALMFVGPRLHSVSTLAVRRMLGREDPLLSPAIQKKLNP
                     PSPPKMILDWLLTALGWSLAISVSAWAVGTVLGVYHFRQIAAWGWLNTILIIPLVWLV
                     LVLGLLKTLVSTIVPSYGGLVGRPLAWLSDRLVDVVDRLQILPGSGTIMPTIPLWLTA
                     VTLGLIGLWTVQPWLRLRRRWLILSATAVLVAWVWHLLPQSRQGTLTLTFPSITNGNC
                     CLIALPNGQTWMYDVGSLAGYDIQRWVVGPLLADKRVFSIDALMLSHANLDHFSGVPD
                     LIDHHNVRRLLVSPLFFDFSTPGDATDRLLADLRRQRIPMTLIGRGSTLPDTGDVTVE
                     VLWPPLGGGLPIRNANDSSLVVRIGYAGYRILLCGDVMEQAQSHLIRQGGLAADVLVL
                     PHHGSTTSDPRPFILAVNPRYCLRCGGPRNSDVSSRLADVLADRVYLDCQIDGAIEIE
                     VGKSGLSVKPWRSSKMSQ"
     gene            18162..19085
                     /locus_tag="P5316_07000"
     CDS             18162..19085
                     /locus_tag="P5316_07000"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR84742.1"
                     /translation="MSVKNLQPEHGSLEFVFEKIEPLAQSEPPATKQERRRAPRRKLH
                     VPVWVRVVIGNHLSPAQARQLIDISASGLGIVSKASYEPGQVMMIELCINNITWSGLM
                     KVVHCSETAGGYKVGLTTITAHVGDAQHQGQLVGRRPSDAATLKQLQEEIPKAMRAYR
                     QARASWGLLGTPIQRNIMRVIANLEPLEEDRPKDTERKHRRLQMKGDVHLVVPTYYGG
                     KWLRAQIIDISEGGAGLLLPFNLTPDDIERELAGHFRIAPGVPVIVGIGTSPNIVWLP
                     AEITRCEKPENGTTRIGVAFNTPASREAFGA"
     gene            19355..20263
                     /gene="metF"
                     /locus_tag="P5316_07005"
     CDS             19355..20263
                     /gene="metF"
                     /locus_tag="P5316_07005"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRR84743.1"
                     /translation="MLFTQLHKQKPLVISFELFPPKTAEAEARLFEQTIPEMLELEPG
                     FLTCTYGAGGSTREETMRMVTRIRQQFGIEIASHLTCIGASRESIAAYLARMRENDIN
                     NVLALRGDPPLNDPNYRPAREGFRLAINLVRYIKGVGGFDIFVAGYPEGHPECPDKYL
                     DWRRCAEKVEAGADGIITQLFYDNNDFFEFDDYLRNKLGVTVPIVPGILPILSTNQIR
                     RFCGLCGAKLPPQVSAQLDRLADDNEACRRYGIDLATQMCDELIRHGVPGIHFYTLNR
                     AASTRAVMQNLGLVAQKQPIEGRESR"
     gene            complement(20371..20868)
                     /locus_tag="P5316_07010"
     CDS             complement(20371..20868)
                     /locus_tag="P5316_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84744.1"
                     /translation="MVARRKSRRRMLLVGGAVLALACVAVVSVLMHLSSGTPGDRKQC
                     AENLRAIGQACISYANANQGNFPRSLRQLLRANGGTLRPDQITCPNAGKKRRGGDYVY
                     VQGYRITDDPNAVLVYEPPDNHKRKPGGHVLLLSGVVNWRDKEQLAEAIAQLKARQAT
                     PMTGR"
     gene            complement(20892..24701)
                     /locus_tag="P5316_07015"
     CDS             complement(20892..24701)
                     /locus_tag="P5316_07015"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRR84745.1"
                     /translation="MKQHLSYPTALFCSWFLVTSVFGQWSSVAPGIDYREYSITMSDG
                     KPNKLFVARMDRSNQSCIIDSTIAMGQLRTGRETVPGMATRYDDTINYWGESWGQRSD
                     VVVAINGDGYTSSPGPTNGQIISGWQAWRWPEYSGGSGFCYKLDRTCFLGGNVRNGNV
                     PGFYYQFIFFANGGNAVPTSVNKVRAGDNELIVYTPQFAEHTYTDNSGTEVLIEMTRP
                     LVCLPVGGPNYVYGTIKQIRPNAGSTPIPFDHIVLSGTGSYATVLQSNCVAGEQIRFQ
                     FEIHDYGTSGQVPTGPSDWTKAYASVGCFAYVVKNGIVPVDDWASNSGAWIRNPRTSV
                     AFNDNYIYFVVCDGRSAQSVGMTYPELGNWLVSNLGAQWATNQDGGGSSTMWVNGQVK
                     NVPSDGSPRAVANGLTMVVVQPKVQSTTFSVAQLVTTNTSSNVRLGPGTNYGILATVA
                     GGTQGQIAPHSLNGVQAKGYYWWKVNFGGSTVGWVAENLLIGGGIAPTITVQPVPQTV
                     PVGGTAAFTVVASGDQPLSYRWQKNGVNLSNGGHYSGVTTTTLTVSSADINDVASYRC
                     VVSNSYGTATSDEALLTLTGQPTITQQPIPQNVCTGGTAVFTIEASGQGTLTYRWQKN
                     DVNLSDGGHYSGSTTPTLTISNADSSDVGSYRCLVTNAAGTTNSNQAPLMIDAIVALV
                     GVGGTDSTITGVSADGTVVAGNNSNGGFIWSVAHGVVGLGTGTSTAGVATKNGNVVVG
                     GLNAGNASRWDGNTAGSGAWTALPLVGGTAAWTPLCTSAQDGSPGNVWVGGNSGSTTG
                     TRQAVRYKESTNSTTALALPPNGNKDAYIYGISDIGSYAGRYQFGNVSPSGSRQAMGG
                     ATLTALWPLIGSPSTSNEGVANTISRDGTKAGGWSQSASFSRQGTIWTIANPTVPFAV
                     PFIGTNDYGEVQALNSDGSVAAGYAQTLAIPGSKHAIIWDATNGTRDLATWLTTRYGL
                     DLNGWTLTDVKGISGDGKVIAGNGTYNGLVRAWVALLGVPGQPPTITQHPTPQIVCAG
                     ADAAFAVAASGLGTLNYQWQKNGADLSDGGHYSGATTPTLSISGADAGDAAGYRCVVS
                     AGCASAISNAAVLTVRPSTTITQQPTARNTCMGLTVEFAVAATGDGAISYRWQKDGVN
                     LNDGGHYSGTTTATLIISDADAGDEAGYRCVVTAGCGTATSDEAVLTLVAKVAPDLDQ
                     DCDVDADDFDLFATCASGPGIIHNGLPLCQMADFDDDNDVDQPDFAVFQRCLRGPGTP
                     ADPNCAN"
BASE COUNT         4588 a         7710 c         7943 g         4861 t
ORIGIN      
        1 gatgggccga cgggggcgtc ggccctaccc cccgcagcct acgcatggcc gtgggcatgg
       61 caggcggcag aggatacggg taagcggtca ggcttctgca aaaacatgcg cgcaaaatgc
      121 gcaccagtcg cgacgataga aactgaagtc tccagcctga agcctgaagt cccgccggat
      181 agcgcgctgg tctccaagcg aaggtcgatg tggggttgtc actccgaagc atttgtagcg
      241 aaggtgggca catccgcgga gcccgcaatg cgccacaagc tgcacgaccg ggtggaaccg
      301 agagcgcacg tcgggcatcg tgttgcttgg cctggctgga agcgttggtt agtatgggcg
      361 tcggctggaa gcaagccgcc cacaggggat agaggaagga ccgacggtat accaatcgga
      421 atagaaaggg cccgaccatg gacctgggca tggacatgtg gaagtactac ggcatcactc
      481 acactgacca cacggtcatg aaccccttga gcctggaaaa gacccgggag ctcgtgggtc
      541 tcttgcggct gccgaacggc gggcacatct tggacgtggc ctgcggcaaa gcagagtttc
      601 tctgcctcgt cgccgaggcg tacgaggtga aggctacggg catcgagctt tccccctaca
      661 ccatcaaggc ggcccgcaaa aatgtggaaa cgcggggcct ggccgaccgc atcgagttgc
      721 tccacacgga cggcggcaat tatgagccga atgcgccaga gagtctggac ctggcttcct
      781 gcatcggtgc ctcctgggtg tacgagaatc acaggggcac gttgcaggcc ctgacgaaga
      841 tgactcggtc cggcggcctg gtgctcgtgg gcgagccgtt ctggatgacc gatcccgacc
      901 cggaatacct gaagctcacg ggcgttgatc ccaacctgtg gggttcccat gccgggaacg
      961 cggccattgg tgaggatgtg ggccttgctc tgctctatac tctggtagcc aacccggacg
     1021 attgggaccg ctacgagggg ctccaatggc gggctgcgga gcgatatgcg gtcgaccatc
     1081 cagaggatcc cgacgtggag gctctgctcc gcagcacccg caggagtcgc gacgcctatc
     1141 tccgctgggg ccggaactgc ctgggctggg ctatgtacct ctttcagaag ccctgaaaac
     1201 agcagcttct ttcaaccggt cgaataatca acctgcacgt tctcgtccgc ctgccgcgag
     1261 caacggcccg aactctctgg ctgtctcgta aacagcggtc gcccagtcaa cagcccgtcc
     1321 gtcccggacc ccaccgccga ttcgaactcg cgcggggaaa gtgagagaag gacgaagagc
     1381 accagtcccg ccggacagcg agccggtcgc caagcgaagg ttgttgcggg cttgcccgcc
     1441 gcagctcctt gggcgaaggc gggcgtttcc ccaaggagcg tgcccatgca tacgagcgac
     1501 ctgtccgcag cagccttgac ggaggcgaac gttccgccgg ccgccgacga gcgggtccgc
     1561 gaagcttgcc tgccgaagga cgggcgcaga tgagtcgccg ccatctttgc ctgcggcctc
     1621 ctgcggctgc gcaggcgtcc gcaactcgcg accacatccg acatgtccgg ccggacgtgc
     1681 tcgtttttgt ccgattccgt ttgcccccaa tccttctaca cgtcgcatgc tgtgggatgg
     1741 gctgttgatc gcgttggaat ccgggctgcg agcgggaaat gggccgaagt cgaaaaatgc
     1801 cgcccccgtt cagtgagaag tacgtcatca cgctggtgcc gaagggagag cgcgtgaaaa
     1861 cgaattcgcc aatggggcat tgtgaaaagg gactgtcgcg acagcgagtt gtactccctc
     1921 atcggctcgt cgcttcgcct tcatctttgt ttttcctcaa cttcgccatc gcttcttgag
     1981 ctgggcatcg atgtgactgc ggccgccagg cgggagctcg tcgtcgacga atcccggtgg
     2041 gctgtctgag cgtttctcga gcagcgccag ggctttcttg gggaggaacc gcgtgagctg
     2101 agcgatgctg tgggcatcgc cggcggggta cctcgtgcgg taatagcgga gcgggaagta
     2161 gatgtagagc cagtccttgg cgcgggtaag ggcaacgtaa aacaggcgtc gttcctcgtc
     2221 gatctgcgat tcgtctcttg tggccatgtc cgacgggatc attccgtcgg cggcgtggat
     2281 gatatggacc gacgtccact cgcatccctt ggccgagtgc atcgtgctga ggatcaagta
     2341 gtcgtcatcc aggcacggtg gcccggccag atcggccgtc gagttcggcg ggtcgagcgt
     2401 caggtcggct acgaaccgcc gtcgcgagcg gaagcgcccg gcgatttgtt cgagctgttc
     2461 gatgtcccgg atccggatct gccagttgtc gtagatggtt ttgaagatcg gctcatagta
     2521 ctctctgagc cgctggatct ggaccgccag tgtcggggcg ctgccggtgg cgacgatgct
     2581 ctgggcctcg tcggattctt cgtcgacatt cacgacgcca tagcaggcct ttaatgcctc
     2641 gcgcaggtcg gcgatcgact ttttcgagtc cggcggcacc ttgagattgt atgaccagaa
     2701 acggcttaat ggcgagccca tttcgccgga cgcttcctgc tcatctccct gggcgggcga
     2761 ctgtcgccgg acacccaact gctgcatcac ccgccgcgcc gactgcggcc ccatgcctcg
     2821 catcatgcac agaatgcgga accaacttat ttcgtcgtga gggttctcca gcacccgaag
     2881 gaaagccagc atgtccttga tgtgggccga ctcgatgaat ttcaggccgc cgaacttgcg
     2941 gaacgggatc cgccgctgca tcaattcgac ttccagggcg tcgctgtggt ggcccgcgcg
     3001 aaacaggacg gcctgctgcc gcagcggaac gccctgttcg aggtgttcga ggatccgctc
     3061 gcagaccaac cgggactgct cgtcttcatc catgcagtaa tacagcaccg gccgtcgctc
     3121 ggccgttcgg gttgaccata agttcttggt gtagcgctcc ctggccggtt ccatcaccgc
     3181 gttggacaga tcgaggatgg gttggatgct gcggtagttc tgctccagtg cgatgatccg
     3241 cgtcccgggg aatcgctgcg ggaaatcgag gatgttgcgg atcgtggccg cccgaaaaga
     3301 atagatcgac tgggcatcat cgccgaccac gcaaatattg ttgttccgcc gccgcatgcc
     3361 gaccaggatg tcggcctgca cgacattggt gtcctggtac tcgtcgacga ggatgtggtc
     3421 gaatcgtccg gcgacccttt cgccgacgcc cggcacgtca cacatcagtt tccagtgcag
     3481 gagcaggtcg tcgtagtcca tcaggttgtt ggctcgcttg cggtcgacgt agagatcgaa
     3541 gatcgcgatc agctctttct cgaactcgcg gcaccagggg aaatgcagat ccaggacgtc
     3601 gaccagtttg tcctgagagt tgaccgtccg cgagtagatg gcgacgatcg tgttcttgtt
     3661 gggaaaccgc tttttctcct tggcggcgcc gagttcgttg cggatgaggc tcatcatgtc
     3721 ggcggcgtcg ccctggtcca tgacggtaaa accgtcaggc aggccgagag cgcggccgta
     3781 gattcgcagc aggcggttgg acacggcgtg gaaagtcccg ccccacacgc ggctcgagac
     3841 cttcaggccc gtctgttggt cggcccggga gatcatctcc aaggccgctc ggcgggtgaa
     3901 cgtcagcagc aagatccgct ccggcgaaac gccgcgatgc aaaagctcgg ccacgcggct
     3961 ggccagcgtt cgcgtcttgc ccgtacccgc gccggcgatg atcagcagcg ggccgtcgcc
     4021 atgcttgacg gcttctcgct gctgctcgtt cagaccttcc agccacggtt cggacataac
     4081 gccccctgaa acgttcccac cgatgagcat cggctccgct atggatctgt acagcgtacc
     4141 gaactcggcg cgaaatgagt agtcgattga ccgcaagaca ccaaaccggc caattctcgg
     4201 cagatgtctt tcacgtcagc agggccttat gatttaattg cctgcgaggt gagtcatgac
     4261 ctaccgaggc aaggtgaagg acggcgtggt ggtcctggat ggagatgtga agctgcccga
     4321 tggtgccacc gtggaagtca gtctgccgga gcggtccgcc aatgaggagg ctcccaccct
     4381 gcttgagcgg ctcgggccgg tgatcgggaa agccaaaggg ctgccgcccg acgcgtcaca
     4441 gaacatcgat cgcgacctgt acgggcaggg taagcgatga atccacggtt tgccgacacg
     4501 gcattctatg tcgctctggt caacccgcat gacgagttgc acgtcgccgc gatagaattg
     4561 gcccgcgact ttcgcggaac cattaccaca accgagtatg tgctcgtgga gcttggcaat
     4621 cggcttgcga ggtcggggga caaagaagtc ttcatcgaac ttgtcgggca actgcaagcg
     4681 gatgctcgca cgatcgtcgt ccccggagac ccatcgctgt tccggagagg tctggacttg
     4741 ttcgcggatc gactggacaa ggaacggtct ctcaccgact gcacttcatt cgtggtgatg
     4801 agcgaacagg ggattaccga cgcactgacg ggcgatcacc acttcgagca ggcgggtttt
     4861 gtggccttgc tcaggtgacc ttgccgcacg gaggcgcacg agcgtcggcc ggcaggcacc
     4921 ctgacgatcc aggcagcagt tcgatgcctc ttcccgccag acacgatcga gtccataatc
     4981 tgcgatccgg tgagcccatc tgcggttccg cagtcggcgt ctgagaacaa gaagacactg
     5041 cggatcccag gcgtgacgag gaggcattct gtctgatgat tcaacgatgt gtgcgaatca
     5101 cccctgcact tctgtcagct acagcgtctg ccttcttggc gacaaccggc acgagcggag
     5161 ccgcgtcacc cgagccggcc gccgcagcga acaccgcccg ctggatctgg tccgacccgg
     5221 ccgatccggc accgaagaac cggttcacct ggtttcgcaa ggtcgttgaa ctgaacgaca
     5281 tgcccgccga tgcgacactc cacctggcgg ccgacagcaa tgcgcgggtg tggatcaacg
     5341 ggcagatcgt ccgccgcaag gtggcccgct accatgagga ccgcatcacc accgagcgga
     5401 tcaacgccgg gccgtatctg cggccggggc cgaatgtcat tctcgtcctg catcacaact
     5461 ggggcgacat catcaccttc cagcggaccg gcaacaagca cgcgggtctg tatgtctcca
     5521 gtacatgggt caacagcgac gcttcgtgga aatgtctcaa ggccgcggag tacatggaac
     5581 atgagaaaca gatccccggc gtgattggcg acaagcgaat ccgttatccg gtgatcgccg
     5641 acggcagcaa ggtgatcgcc ggcaacctgc acgacccgat gtttgacgac ggcacatgga
     5701 aaccggcggt ggtcattgag aacggtccct ggccggccag gccgagcaac gtcgaaacac
     5761 ccggccagcg agagtatccg gtacggcctg ccaaaatcct ggccgcgggc acggctgagc
     5821 ggacgatgct gatctcggat gatccggctt ccatggccgc agggattcgc acctcgaaat
     5881 gccgccccga gacagagttg acgggcattg cgggcgacct gatcagcgga aagccgataa
     5941 ccattagcgg ccaagcgggc gaaacaaagt acatcacgtt cgatttccat cagccgatac
     6001 acggttaccc gttcatcggt ctgaaagacg ctccggccgg tgtgaagatc gatctgggct
     6061 actgcgaact gtctcgctcg ctgtgcaccg gtaagctgca cgtcgacgag accggctgga
     6121 tcaacccgga aggcgtcgtc ggaccgggat acgcggaccg ctatatcacc cgggctggta
     6181 cgcagtcgtt cgagttcccc gatgagcgga ccgcccgctg gctctcgctg cacatacact
     6241 tcgcagccga cggccaagtg atcctcagcg atgtcggcat caccaagtcg cagtatccga
     6301 tcactcctgt gggcagcttc cagtgcggca acgagctgat gcaccagatc gtcaagctgt
     6361 gtctcatcca cgctgaggtg accatgtcgg acacctacgt ggacacaccc ggccgtgagg
     6421 acggccagtg gatcgaagac aaccgcccgc gggccatcct cgctgcccgc tggttcggcg
     6481 acaaccgact gaggcgtttc atgatccgca cctttgccca aggccagggc aaggacggcc
     6541 agttccaccc cttcgccccg agcaactacc ctgcgtatcc ggcgacatac gactggggcg
     6601 ttgaatggac cgccgctctg tatgacgact acatgtggac cggccagacc gatctggtca
     6661 gagaatactg ggacacgctc cgacggttct gggacaattg cctcacgcac gtcggtgatg
     6721 acggcctgtg gcgcacacca cacatattcg ccgatattcg caacagcttg cgtccggcca
     6781 atgacaagca gtccagcggt attgtcaccc cgtggatcat ccagcgactc ggatggtcgg
     6841 tcgaaatggc cgaggccgtc ggcaagaccg agcaggccgc aaagtggcgc gaaacccgag
     6901 agaaaatgat cgtcgcgttc gggaagtacc acatcgtacc gcccaagggc gacgtccctc
     6961 ctcacgtcgg cgaccgggtg gacccggccg acccgaatct cgatcgaggc tactgccagg
     7021 ctggacagac gatcgccatc atcgccgggt tgctcgatta ctcggaggcc atcgcggact
     7081 tgaactatgc ctttcaggat cccgatggct cgcccggccc gggggtgaag aggtggaata
     7141 accccaccta tggttaccgt tcgctgcggg cgctgtcgga caccggcttc acgcaacgag
     7201 cagtcaacca cgtcatcgaa cgctatgcgc cctacctgcc cggtcatccg cgaaaccaag
     7261 tgccgccggt cctgcaaggt ccctacggcg gtccgctgcc cgagtattgg atcagtcggg
     7321 aagatctcgg tctcaaggac ggtgagaaga accccacgca acccgacgat gagaccggct
     7381 cgcacggctg gggcgcgctg ccgctgctgt ggttgcacga gtcgttgctc ggcgtccgca
     7441 ttgtttcacc gggcggcaac aagatccgca tcacccccga agacggcgga ttgccctttg
     7501 tcgcgggcca tacctgcacg cccaagggcg tcgtctgggt ctattgggac ccccagcagt
     7561 ggcggctgga ggtgagtatt ccggctgacg tggaagccga tctggtgctg ccgaagcagt
     7621 gccggggcaa gcgaatcgcg atcgcagaat ctgccgggcc tgtggagccg gcgggtgaag
     7681 aacgatacac gctgagtgct gctggtcgat gcgtgttgat cgccagatag ccgcggagcg
     7741 tctcgtgttc ggtggcagcc cttatctttg tgccactgct ctgtgagcag tgctgagagg
     7801 acgtcggaca ctgctcacag agcagtgaca cacagcggcg tctgcccact ccatcggccg
     7861 ccgcagtttg acggatgcac ccagacaacg gccagacacg ttcacagcct cgacgccatt
     7921 atcgcgtagc cttaacgtgc ccgctggccc cagattccca tctggggcct cttatgagag
     7981 gattctcatc ctcattctcg ccggatgcgg aacagattcc gcgaagagac acggcccgga
     8041 acggattccg ggccgagcgg tcacaacctc gacgacgctg ccgggcggcc gtatcatgcc
     8101 cctcgtaacg tcggagagcg gcgatgccgg ttcgtttcgt ggttggtcga tcggggagcg
     8161 gcaagacgtg gcggtgcctg gaggccgttc gggcgcggct gcgcgaagac cccgtcggcg
     8221 gcagcaagct gcttctgctg gtacccgagc aggccagctt ccagatggag cgggcgttga
     8281 tcgagacgcc ggacatccac gggttcatcc gttgcgaggt cctgagtttc cagcggctgg
     8341 cctatcgcat ctttgccgaa acaggcgccg acccgcggcg ggcagacgag accatcggcc
     8401 cgctcggccg gatgatggcc attcgccggc tgattcgtcg cgagaagaac aacctgaggc
     8461 tgctcggccg ggtggccgac aagcccgggc tggttcgaca ggtgtcatcc gcccttgatg
     8521 agctgatgcg ggccgatctt gagccggcga cgctggccga gcttgctgag cgatacgagg
     8581 gtgccaaccc gttggcggcc gcgaagctgg ccgacctgac ccgcctctac cgggcctatc
     8641 tcgattacct ggaggatggc cggctggatc cggcgcagta cctgcacttg gctgccgaac
     8701 ggctcgacgc ctgcacgtgg ctcaacggtg cggaggtctg ggtcgacggc ttcgcagggt
     8761 tcactcagca agagtacgtc cttctggtca agctggccca gcgggccgcg ttcatggaga
     8821 tcgccttgct cgtcgatccg cgggcttccg ccattgaagc gatcgaactg ccgaagacga
     8881 cgttctcact tttcgcccgc accgaacgaa cgctggttcg actgcgcagg gacctgacga
     8941 ccgccggtgt ccccatcgcc gaaccggttc gactggaccc gtggccaccg ccgcgattcg
     9001 tcgcccccga actggctgcg ctcgaaaagc atctcttccg caccggctct ccggaggaca
     9061 ccggcgcagc gccgacggcc atcagcctgc tggagtttcc cgatcgccgc tgtgaggtcg
     9121 atgccgcagt tgctgagatc cgccgtctca cgcaactgcc cgatgctccg ctgcgatacc
     9181 gggacatcgc ggtcgtggtg cgcgacttga ccgagtacca cgatcttctc tcggcatcgc
     9241 ttcgagcggc cggcgttccg ttcttcatcg accggcgaca accaacgacc aaccacccgc
     9301 tgatcgagct ggttcgcggg ctgctgtccg tcgcgtcgga cgattgccgg ctcgaatccg
     9361 tgaggttgac tctcaagacc ggcctgttgc cgatcggcga cgacgatgcc gatacgctgg
     9421 agaactattt gctggctcac gggattgccg gccgggacgc atggcgggaa ccctggacct
     9481 tcacccggat gttcaggcgc cgcggccgga aagcaggact cagcgatgcg cagaagagca
     9541 tcctgaatcg cgtcaaccag actcgacagg catggctcga cgcagttggt ccctggctcg
     9601 aaaccgccgc ggccccggaa gggcatcggg gccgcacgtg ggcgtcgtcg ctgtttgcct
     9661 gcctggaacg aatcggcgcg ggcggtcacc tggaacgttg ggcacgcgac gcggaattgg
     9721 atggccgcag cgatgaagcc gaccaccacc gtcaggtctg ggtggatttc gtccggttcc
     9781 ttgatgagtt tgtaatggca ctgggcggcg acaggatgag tatcaacgaa ttccgcgaga
     9841 cggtcgaggc cggcctggcc gagttcgatc tggggttagc cccgcccacc ctcgaccagg
     9901 tgctggtcgg agccatcgaa cgaagccggc atccgccggt ccgtgcggtc ttgctgctgg
     9961 gtttcgacga gcagcatttc ccgatgcggc gcaccgagga tccgcttctg ggcgatctcg
    10021 aacgcgacga tctcgaccgg gcaggcatgg agataggccc gtcgcgtcag cgacagttgc
    10081 ttgatgagcg aatgctcgcc tatatcgcca tgactcgcgc cggcgagcga ctctggatca
    10141 gctatccgcg cgccgccggc gatggcacac ccgttgagcc gtcggcgtat cttccggact
    10201 tgctcgcggc agtgccggga ttgcggcctc aacgatgcga cgacccgcga ttgacccgca
    10261 ccctcgcagg catcagccga gtgcccgagc tggcggagcg tctggcgcag gaattgcgtt
    10321 ggcgtcccag ggcgccagac gaggttgacc ccgcagccag agcacgatgg aacgcgattt
    10381 acgaggccgc aagacagcac gttacgtggc agcacacgct gagaatgtgc ctgtcgggtc
    10441 tggcgtatcg caatcaggca aagcttgaac cgggcctcat ggagcaggcg gtgccctggc
    10501 cgttcgccgc cagcgtcagc cgtctggaga gatttgcggc atgccccttc gcgcactttg
    10561 ccgagcacac actggccctc gaacagcgca tggaggctga cctcggcacg ctcgatctgg
    10621 gcaatatttc ccacgcggtc ctggagatgt tcattgcgga gctggccagg gagggccgtc
    10681 gccttggtga ccttgatgat gacgaggtca ttgatcgcat cgatcgtacc gcggccgaac
    10741 tggcacccaa gttggggggc gacatgatgc tggcggaggc ccgcaacgcc tatctcttcg
    10801 atcgcagcag gaatcatctg caccgcgtga cccagtggca gcgcgacgca gccaggtccg
    10861 gccgttatta tcccgtccgt actgagcggg aattcggcta tcgggagaac gagaaagtgg
    10921 ttatcaccac gccccgaggc cggcgcgtgg agcttcacgg catcatcgac cgggtggacg
    10981 tcgtcgagct ggccgactgt tatggcgcgg tggtcatcga ctacaagacc gtcagcgagc
    11041 gcaggctgaa gatggcggag gtctatcatg gattgaccct gcagcttgtc ggatacctgc
    11101 tggcgctgca acaggccggt gagtcgctca ccggtagaaa agtccggccg gtggcggcgt
    11161 tctacttgcc gctgttgcag ggcttcgaga cggtatctca tccttcgaag gcgacttccc
    11221 cgacggaggc ttaccgtttc cgcgggattc tcgacctgac ggcactcgat gggctggaca
    11281 cgtcagccgc aagcgatcgc aagtcacgat acatgagcgc tggtctcacc aaggacggca
    11341 agccacacca gaacagcgat ctggcaagcc cgaaggagtt cgaggcaatc atggctcacg
    11401 tcagccgcaa gatgggagag ctggcggatt cgttgctcga tggcaacgtc gaggtgaagc
    11461 cctatcggct caacaaaacc tcgccgtgca aatggtgccc ctacatggcc gtgtgtcgat
    11521 acgaagcgga ctggcaaccg cccaacaccc tggcccctct gggcaggcgc gcagacatac
    11581 tcaagcgatt gacggaggag catcccgatg cctgacgcag caccccgcga tcgctacgcc
    11641 cacctcacgg aaagacaaag agaggcgatc cagaccactg gccggagctt gctggtctcg
    11701 gccgcggccg gagcaggcaa gaccaccgta ctggcggagc gatgcgctta cctgatttgc
    11761 gatttgccca aagccgaccg ttgcggcatt gacgagttgc tggtagtcac gtttactgat
    11821 gcagcggcgg gggagatgcg tacccgcatc cgaaaggcca ttcgccagcg ccggcaggaa
    11881 tcatcacatg acccctggct ggaccagcag attcaccttt tggagactgc gagcatttcg
    11941 acgattcact cgttctgcaa aacggtaatt cagcagtggt tcccgcaggc cggcgtcgat
    12001 ccgcaagcgg cggtcctggc cgaggatgag gctgatttac tccgacatga gacaatcgat
    12061 gctttattcg tcgagcttta cggtcgcaac gacgaatatg ggcgagattt ccagtcgctg
    12121 gtcgatgact atgggtccgg cgacgacgcc agaatcgccg aggctctact ggcaatccac
    12181 gctttcatcg ccagtctgcc cgaaccacag gcctggctcg accgcgccgt gcaggttctc
    12241 gaacccggcg cgccgcaaag cctcgcagac cggatcgatg agattcagca ccggcggctg
    12301 atccgcgaac tggagcttca cgtccggcaa gcccgtgaga gcgcgagagt catcgactcg
    12361 ctgtaccccg ttgcttccat gcacgccgac gccctgcgtg agtacgtttc gcagtgtgag
    12421 gcgtggctcg accgcctgaa cgccgggagt caggacgcct ggggggagat tgcccgcgaa
    12481 gttgcgggac acacgatcgc caccgaaaga cgtccgagca ggctccccga agcgcaggtc
    12541 gctcagttcg acgcggccaa gaaaatcgtc gaatggcaca gggacctgct ggagaaacgc
    12601 ctccaagacg gcatctgctc gttttccaag aacgaataca tcgagggttt gcagcggatt
    12661 ttgccgtacg tgcggaccat cgtgcgactg gtcaaggatt tcggcgaccg ataccaacaa
    12721 gccaaagccg tccaggccgc cgtcgatttc aacgaccttc aacgtcaagc ctattggctg
    12781 ctcagccggg atggcgaccc caacaggcct tcggaggttg ctcttcaact tcagaagcgg
    12841 tatcggcatg tcctggtgga tgagtttcag gacgtcgatc cgctgcagga ggcgatcctg
    12901 cggctggtca gccgtgaaac cgccgaaccg cctgagggca acctgttcgc ggtgggcgat
    12961 gtcaagcaga gcatctaccg cttccgattg gccgagccgg cgttgttcac ccgccgggcg
    13021 gatgcattct cctccgagtc aaccattggc aggctgattc ctctgggcga caacttccgg
    13081 agtcgcgaag gcatcattga cgcggtcaac cttgtgtttg ctccgctgat gagccgctcg
    13141 ttcggaggaa gcgactacga cgagaccgcc cgtctgcgtg ccggtgcctg ctatccgccg
    13201 agcgaagggc tgcgggtgtt cgacgggccg gccgtcgaac tgcatctgct cgagccggtc
    13261 agaggtatgg ctcaggcagt cgatgcgggc gacgaaggaa ccggcgatcc cggcgcgacg
    13321 caaggtgatt cgcagcagga tgaactggcc ggcattgaac gggaggcttt cctgatcggc
    13381 cggcgaatcc gggattggat gggatcaagc ggcgcacgga tgaacgttgc cgaccggccc
    13441 gccacgcccg gaggaccgcc cgttctgcga cccatcgaat atcgcgacat cgtcattctg
    13501 cttcgctcga tgccgcacaa ggctgagccg atcgccaaca tcctccgccg gatggggatt
    13561 cccgtgttga tcgggcgagg ggaaagcccg gccgactcga cggagttccg tgacgttctc
    13621 agtctgctgc gcgtgctcga taacaggcag caggacattc cgctggcggc agtgctgaga
    13681 tcgccgctcg tcggccggcc gttgactgag acgcagatgc ttcgcgtcag gctgcacgac
    13741 ggcgaggttc cgtttcaccg ggcggtcatg cggtatgctg agaacgggcc tgatgaggag
    13801 ttgcgatatc ggctttccgt gatcctggcg acactcgatc gctggcggac gcgcattcgc
    13861 cgcctgcccg ttgcggaggt tctgtgggac attcttgagg agacgcacta tccggcgttt
    13921 gttgccggtc ttccggacgg tgctcggcgc cgcgaacggc tcatccagat tcacaatctg
    13981 gcgcgagact tcggtcagtt ccgcagacag ggacttcggc gatttcttcg attcatggat
    14041 gaactgatcg cggccgatcg gtcgccgcaa ccagccgcgg gctccggcga agatgataat
    14101 gcggtccgaa tcatgaccgt ccacaccagc aaggggctcg agttccccgt ggttatcgtg
    14161 gcggacctgt ccaagtcgtt caacctgtct gatgtcaagg aaagcgttct ggtccatcgc
    14221 aatctgggaa tcgctcttcg agccgcggac cccgagaggc ggatccacta cccgacgttg
    14281 attcaccagt tggccgttga agacgaccgc agagagagcc tgtcggagga gcttcgtgtc
    14341 ctgtacgtcg ccgtcacacg tgcccgcgag catctggttc tggtcggccg cattcaaccg
    14401 gaacggctgg ccgtgttccg catgccgcga gaaccatccg cagagaagct gcctcgcctg
    14461 caactcgaag tggcgaacca acccctcact tggttgctgg cggcgatcgg ggcggcaccg
    14521 gccgacaatg tccgatggcc cgaggacgcc ggggcatccg gctccatcct gattgaggtc
    14581 catcagtatc ccagggccga gaccgatcat tggcggacgc cgcccgcagt cgcgcccgag
    14641 cggctcgacg ctctggccaa ctgtgcccgg cttggtcctt tgccgggcga tgagcctgtc
    14701 cagggatctg atgaagcccc gcgactgatc gcctctctgg atgcgccgta tccagccctc
    14761 gaactcacga ccttgccagc aagggttgct gtgactgaac tgaaacgccg atgggacgct
    14821 tggagcgatc ccgacctgcg accaggcaag ccgccggtag ccggacctgc accggccgtc
    14881 ccggtcttca tcgagccgtt gcctgttgcc caagccgtca atcgcggaat ggcgactcac
    14941 cgtttctgcc aactcatcga cttggccaga ccctgtgatg ccgccgatct cgctgcgcag
    15001 caccgcttct tgagggaggc cggacgcctc agtgaggccg aggcctcagc ggtgttgctc
    15061 gatgacgtcg cgtggttctt ctccacggct cttggtcatc acatccgcaa gcatgctggg
    15121 agggtgcgac gggaaatcgc ctttgtgtcg cggatcatac ctgatcgtta cgagcccatg
    15181 gttaccgctc gcgattcccg agacgtgatt ctggtcaggg gtacggtcga cgtgctgttg
    15241 tgccacgagg atcacctgga aatcgtggac tacaagaccg atgcgatccc acatgaagaa
    15301 tgcacagagc ggtccagact ctatcaggaa cagcttgaca actatgcaaa ggccttgcat
    15361 ggtattttcc agcgtccagt agttggccga tggctggtat tcctgcaagg ccggtgcgtc
    15421 gttgccctcg ctgctccaac agcctgatct gctgccgacc tcacggcttt cacatttgcc
    15481 cgtttctcgt cgtgccgaac tggcggtctg gctcaacgac gagccgctgg tgctgccggc
    15541 ggcggcattg gcggccggca tatggcttga tgccgtctgg ccggtgccag gctggttgtc
    15601 gatgttactg ttcctggcgg caggtggctt gctcttgata gctcgccgcc gtccgtggtt
    15661 cgtatacgcc gttctgcctc tggcggcgct atcgaccgga gcagcggtgc atgatcttca
    15721 ttttcggcgg cagccgacga atcacgttgc tcactactgc gcgcgcgagt cacttcgggt
    15781 tcggatgacc ggaaccgtgt tggcgccgcc gcagattcgc tcggccggta ccggtcggat
    15841 cacctggttc aaggaactgc cgcgtacgtg tctgctggtg gaagcggaac atctcgagcg
    15901 ccgctccggg ccgctgaccg tgtgtgggat gatcgccgtt cacgttcgcc agcctgttct
    15961 ccatatcacg gccggagatc gcgtcgagat catcggcact ctctatcaac cgccgccgcc
    16021 cgacaatccg ggcgaaaaag actggcgatt gatattgcga cgcaaaggca tcctcgcgga
    16081 gatgtcatgc gagcgggacg cggaccttgt ggtaaagggc gtctcagatc cgggccgtcg
    16141 atggatcgca ggtctcaggc gtcgcctgaa ggccgccatg atcgatcagg cgatgcccgg
    16201 cgatatgccg ggggccaaga tgctggccgc cctggtgctt ggacagcgca gcgccgtgga
    16261 tgacaggctc aaccaggcct ttgtcaacac cggaacggtc cactatctca gcgtgagcgg
    16321 ggcacacgtt gccatgctgg cgtcggtcgt gtggatgatc gggacggtgg ccggggtgtc
    16381 acgcaggtcc tgtgcgctct gggccatgat gctgatcatc ggctatgctc tcttgaccga
    16441 gcccgagccg cccgtcgtgc gatcggccct gatgggaacc ctgttgtgca tctcggtcct
    16501 gatgcgccgg ccggttcgat cggccaactg gcttgccctg tccgcgatca tcctcctcat
    16561 cattgcgccc ttgcagttgt tcgacccggc ttttcaactt tcgtacctga cggtgccggc
    16621 gttgatgttc gtcgggcctc gtctgcatag cgtttcaacg ctcgcagtgc gacggatgct
    16681 cggccgcgag gatcctctgc tttcgccggc gatccagaag aagctcaacc cgccgtcgcc
    16741 accgaagatg atattggact ggttgctgac cgctctcggg tggtcgttgg ccatcagcgt
    16801 ttctgcgtgg gcggttggca cggtcctggg cgtttatcat ttccggcaga tcgccgcgtg
    16861 gggctggctg aacacgattc tgatcattcc gctggtttgg ctggtgctgg tgctgggcct
    16921 gctgaaaacg ctggtgtcga caatcgtccc ttcgtacggg gggctggtcg gtagaccgct
    16981 ggcctggctc agcgaccggc tggtcgatgt ggttgaccga ctgcagatac tccctggatc
    17041 gggcacaatc atgcccacga ttcctctctg gttgaccgcg gtgactctgg ggctgatcgg
    17101 actgtggacc gtccagccct ggctgcgctt gcggagacgg tggctgatcc tatcggcgac
    17161 ggccgtgctg gtcgcctggg tctggcatct cttgcctcag tcgcggcaag gcacgctcac
    17221 gctgaccttt ccctccatca ccaacggcaa ttgttgcctc attgcgctcc cgaacggcca
    17281 aacgtggatg tacgacgtcg gctcgctggc cggctatgac attcagcgat gggtggtcgg
    17341 gccgttgctg gcggacaagc gggttttctc gatcgacgcg cttatgctga gtcacgccaa
    17401 tctcgaccat ttcagcggcg taccggatct gatagaccat cacaacgtcc ggaggctgtt
    17461 ggtatcacct ctgtttttcg acttcagcac tccgggtgat gcgactgatc gtttgctcgc
    17521 cgatctgcgc aggcaaagaa tacctatgac cttgatcgga cgcggttcga ccctgcctga
    17581 tacaggcgac gtgaccgtgg aggtgctgtg gcctcctttg ggcggcggtc tgcccatcag
    17641 aaacgccaat gacagctcac tcgttgtgcg gatcggttac gccggttatc ggattctgct
    17701 atgcggcgac gtcatggagc aggctcaaag ccatctgatc cggcagggcg gtctggccgc
    17761 cgatgtgctg gttcttcctc atcacggcag caccacgagt gatccccgcc cgttcatcct
    17821 ggcggtcaat ccccgatact gtcttcgttg cggcggcccg cgcaatagcg acgtatccag
    17881 tcgcctggcg gatgtgcttg ccgatcgtgt ctatcttgat tgccagatcg atggagccat
    17941 cgagatcgag gttggaaaat cgggcctttc ggtcaaaccc tggcgcagct cgaagatgag
    18001 ccaataggtg acgtcgtggg gttcttgtta tcggctgccg gacgttattg ggtcgcaagg
    18061 tacttaccgg acgcggtccg caagttgccc cgtttgcctg cctgtccaat gaatcgtatc
    18121 ttgactctcg tgcagatgtg tgggttaaag gggggcacgt gatgtcggta aagaacctcc
    18181 agccggaaca cggatccctg gaattcgtgt tcgagaagat agaaccgctc gcgcaatcgg
    18241 aaccgccggc gaccaagcag gagcgccgga gagctccccg gcgcaagctt cacgttcctg
    18301 tgtgggttcg cgtggtgatc ggcaaccacc tcagtccggc acaggcccga cagttgatcg
    18361 acatttcagc gagcgggctg ggcatcgtct ccaaagccag ctacgagcct ggtcaggtga
    18421 tgatgatcga gctgtgcatc aacaacatca cctggtccgg cttgatgaag gtcgtgcatt
    18481 gcagtgagac ggccggcggc tataaggtcg ggttgaccac aatcaccgcc cacgtgggtg
    18541 atgctcaaca tcaggggcag cttgtcggcc ggcggcccag cgacgcagcc actctcaaac
    18601 agcttcagga agagattccc aaagcgatgc gggcatatcg gcaggcccgg gcctcctggg
    18661 ggctgcttgg gactccgatc cagaggaaca tcatgcgggt tatcgccaac ctggagcctc
    18721 tcgaggagga tcgtccgaag gacaccgagc gcaaacaccg ccgcctgcaa atgaaaggcg
    18781 acgtccactt ggtcgtgccc acctactacg gcggaaagtg gctgcgggcc cagatcatcg
    18841 acatcagcga aggcggggcg ggacttttgc ttcctttcaa tctgacgccc gacgacatcg
    18901 agcgcgaatt ggcgggtcat ttcaggatcg ctcccggggt accagtgatt gtcggcatcg
    18961 gcacctcacc caatatcgtc tggttgccgg ccgagatcac gcgctgcgaa aagccggaaa
    19021 acggaaccac gagaatcggt gttgcgttca atacccccgc ctcacgagag gccttcggcg
    19081 cgtaagagcg tcgggggcgt acagatcgta cgcgccacct gcgcggcgtc gccacagggg
    19141 ccgtacatga cgcggcgcgc gttccgcaac catgtcttgg cataatggca gcgagattca
    19201 atgattcgca gcggctgagc tgtgtgtttc gtttgccaca aggcacccag ggctggacgc
    19261 ccatccgtca gtcggtttta tcgaggactg atgcaatcag gtcgatataa tcgtgacggc
    19321 cacggacgct ggaccttcct gatggagatt tgcggtgctt ttcacgcagt tgcacaagca
    19381 aaaacccctg gtcatttctt tcgagctgtt tccgcccaaa accgccgagg ccgaggccag
    19441 gcttttcgag cagacgatac cggagatgct cgaactggaa ccgggctttc tgacttgcac
    19501 ttacggggcc ggcgggagca ctcgcgaaga aaccatgcgg atggtcaccc gcattcgcca
    19561 gcaattcggc atcgagattg caagccacct tacctgtatc ggggcttcgc gtgaaagcat
    19621 cgccgcttac ctcgccagga tgcgggagaa cgacatcaac aacgtgttgg cccttcgcgg
    19681 tgacccgccg ttgaatgacc ccaattaccg cccggcccgg gagggttttc ggctggccat
    19741 caacctggtt cgctacatca agggcgttgg cgggttcgac attttcgtgg ccggctatcc
    19801 ggaggggcat cccgagtgcc cggacaaata cctcgattgg cggcgctgcg ccgagaaggt
    19861 cgaggccgga gccgacggga tcattaccca gctattctac gacaacaacg acttttttga
    19921 gttcgacgat tatctcagaa acaagctcgg cgtcaccgta ccgattgtgc ccggcatctt
    19981 gccgatcctg agcaccaatc agatcaggcg gttctgcggg ctatgcgggg caaagctacc
    20041 gccgcaggtt tcggctcaac tcgacagatt ggccgacgat aatgaggctt gccgccggta
    20101 cggaatcgat ctggccacgc agatgtgcga cgaattgatc cgccacggcg ttccggggat
    20161 tcacttctac accctcaatc gtgccgcctc cacgcgtgcg gtgatgcaga acctcgggct
    20221 ggtggcacag aaacaaccga tcgaaggtcg cgagagccgg tgacggccgg gttgagaaaa
    20281 cccaagagaa tcgccggcgc attcagaccg ccgcaccgga tgcggcagcc cgcccatgat
    20341 ccggtttggg accaccttcg atgttcccgg tcaccggcca gtcatgggcg tcgcctggcg
    20401 ggctttgagc tgggcgatcg cctcggcgag ttgctccttg tcccgccagt tcacaacgcc
    20461 gctcagcaga aggacgtgcc ctccgggctt tcttttgtgg ttgtccggcg gctcgtagac
    20521 caggacggcg ttcggatcat ccgtgatgcg atagccttgc acgtacacgt agtcgcctcc
    20581 gcggcgtttc ttcccggcgt tggggcaggt gatctggtcg ggccgcagcg tgccgccgtt
    20641 ggcgcgaagg agttgcctga gcgagcgggg aaagttgccc tggttcgcat tggcatagct
    20701 gatgcaagcc tggccgatgg cccggaggtt ctcggcgcat tgcttccggt cgcccggcgt
    20761 tccggaggag aggtgcatca aaacgctgac gaccgcgacg caggccaagg ccagcacagc
    20821 ccctccaacc agcagcatcc gccgtcgact ttttctgcga gcgaccatgc cagcctctgg
    20881 ttactgcctt ctcagttcgc gcaattcgga tcggccgggg tgcccgggcc tctgaggcat
    20941 cgttgaaaga cggcgaagtc cggctgatcg acgtcgttgt cgtcgtcaaa gtcggccatc
    21001 tggcacagcg gcaagccatt gtgaatgatg cccggcccgg aggcgcacgt ggcaaacagg
    21061 tcgaaatcgt cagcgtccac gtcgcagtct tggtcaaggt cgggcgcgac cttggcgacc
    21121 agggtcagga ccgcctcgtc agaggtcgcc gttccgcagc cggccgtgac cacgcaccgg
    21181 tagccggcct cgtcaccggc gtccgcgtcg gagattatca gggtcgccgt cgtcgtgccg
    21241 gagtaatgtc caccgtcatt caggttgacg ccatcctttt gccatcgata ggatatcgca
    21301 ccatcgccgg ttgccgcaac ggcgaattca acggtgaggc ccatgcacgt gttcctggcg
    21361 gttggttgct gggtgatcgt tgtggacggc ctgacggtga ggaccgcggc gtttgagatc
    21421 gcgctggcgc aacccgccga caccacgcag cgatagccgg ccgcgtcacc ggcgtccgcc
    21481 ccggaaatgc tcagcgtcgg agttgttgcg ccggaataat gcccgccgtc gctcaggtct
    21541 gccccgttct tttgccactg atagttcagc gtgcccaagc cggaggcggc aacggcgaac
    21601 gccgcatcag cacccgcaca gacaatctgc ggcgttggat gctgggtgat ggtcggcggt
    21661 tggcccggca cgccgagcag cgccacccac gcccgaacga gaccgttata cgttccattg
    21721 cccgctatta ccttgccgtc gccggagatg cccttgacgt cggttagcgt ccagccgttc
    21781 aggtctagac cgtaccgcgt agtcaaccag gtcgccagat ctcgcgtacc gttggtcgcg
    21841 tcccagatga tggcgtgttt ggaccctgga atggccaatg tttgggcata tccggcagca
    21901 accgatccgt cactgttgag cgcctgcacc tcgccgtaat catttgttcc gatgaacggg
    21961 accgcaaacg gcacggtggg gttggcgatc gtccagatgg ttccctgtct tgaaaagctg
    22021 gcggattggc tccagccgcc ggctttggtg ccgtcccgcg agatggtatt tgcgacaccc
    22081 tcgttgctcg tgctgggcga gccgatgagc ggccaaagag cggtcagcgt cgcgccgccc
    22141 atcgcctgac gcgaaccgct tggcgagacg ttgccgaatt ggtatcgacc ggcataactg
    22201 ccgatatccg agatcccgta gatgtaggcg tccttgttac cgttgggcgg cagggcgagc
    22261 gccgtcgtgc tgttggtgct ttccttgtag cggaccgcct gcctggtgcc ggtggtggat
    22321 ccggagttgc ccccaaccca gacgttgccc ggcgagccgt cctgggccga cgtgcacagg
    22381 ggtgtccaag ccgctgtgcc ccccacaagc ggcaacgcgg tccaggctcc gctcccggcc
    22441 gtgttgccgt cccaccgcga ggcattaccg gcgttgagcc cgccgacgac cacgttgccg
    22501 ttcttggtgg cgacgcccgc cgtcgatgtt ccagttccaa gacccacgac accgtgggcg
    22561 acggaccaga tgaacccgcc gtttgagttg ttacctgcca cgacggtgcc atcggcgctg
    22621 acgccggtaa tggtactgtc tgtgccgccc acgccgacaa gggcgacaat ggcatcgatc
    22681 atcagcggcg cctgattgga gttggtagtg ccggccgcgt tggtcaccag gcaccgatag
    22741 ctgccgacgt cgctgctgtc ggcgttcgaa atggtcaacg tcggcgtggt cgagccggag
    22801 taatgaccgc cgtcggacag gttgacgtcg ttcttctgcc atcggtaagt cagcgtaccc
    22861 tggccggatg cctcgatggt aaagacggcc gtcccaccgg tgcagacgtt ttgggggatc
    22921 ggctgctgcg tgattgtcgg ctggccggtg agggtcagta aggcttcgtc ggacgtcgcc
    22981 gtgccgtaag aattgctcac cacgcaacgg tagctcgcca cgtcgttgat atcggcgctc
    23041 gatacggtca gcgtcgtcgt ggtaacgcct gagtagtgcc cgccgttgga taggttgacg
    23101 ccgttcttct gccaccggta ggaaagcggt tggtcgcccg aggcgaccac tgtgaacgct
    23161 gccgttccgc cgacgggaac cgtttgcggc actggctgaa cggtgatcgt tggggcgatt
    23221 cctcccccga tgagcaggtt ctcggcgacc cagccgacgg tgctgccgcc gaaattcacc
    23281 ttccaccagt agtagccctt ggcctgcacg ccgttcagcg agtgcggggc gatctgaccc
    23341 tgcgtgcctc cggccacggt ggccagaatg ccgtagttgg tgcccggtcc gagccgaacg
    23401 ttcgagctgg tgttcgtggt caccagttgg gcgacggaga aggtcgtgga ttggacctta
    23461 ggctgcacaa cgaccatcgt caggccgtta gcgacggctc gcggtgaacc gtcggaaggc
    23521 acattcttca cctgtccgtt tacccacatc gtcgatgagc cgccgccgtc ctggttggtg
    23581 gcccactggg cgccgagatt gctgaccagc cagttgccca gttccggata ggtcatgccc
    23641 acgctctggg cggagcgtcc gtcgcagacg acgaagtaga tgtagttatc attaaatgcc
    23701 acagacgtgc gagggttgcg gatccaggcg ccgctgttgc ttgcccagtc gtcgacgggc
    23761 acgatgccgt tcttcaccac atacgcgaag cacccgaccg aggcgtaagc cttcgtccaa
    23821 tcagacgggc ctgtcggcac ctgcccactt gtgccgtagt cgtggatctc aaactggaac
    23881 ctgatctgtt cacctgcgac gcagttgctc tgaagaacgg tcgcatagga tcctgtgccg
    23941 gagagcacga tgtggtcaaa gggaatcggt gttgatccgg cattcgggcg gatctgtttg
    24001 atcgtcccgt aaacgtagtt cggcccgccc acgggcaggc agacgagcgg ccgcgtcatc
    24061 tcgatcagca cttcggtgcc ggagttgtcg gtgtaggtat gctcggcaaa ctgcggggta
    24121 tagacgatca gttcgttgtc accggcccga accttgttga cgctggtcgg aaccgcattg
    24181 ccgccgttgg cgaagaagat gaactgataa tagaagccgg ggacgttccc gtttcggacg
    24241 ttcccgccga ggaagcaggt tcgatccagc ttgtagcaga agccgctgcc cccggaatac
    24301 tcaggccacc tccaggcctg ccagcccgag atgatctgac cgttggtcgg tcctggagag
    24361 gaggtgtacc catctccgtt gatcgccacc accacgtccg atcgttgccc ccacgattcg
    24421 ccccagtagt tgatggtgtc gtcatagcgg gtcgccatgc ccggcacggt ttcgcggccg
    24481 gtacgcagtt gccccatcgc aatggtgctg tcaatgatgc agctttgatt actccggtcc
    24541 atccgggcca caaacagttt gttcggtttc ccgtcggaca tggtgatcga gtactcccga
    24601 tagtcgatac cgggagccac ggagctccac tgcccgaaga cactcgtcac caagaaccaa
    24661 gaacagaaaa gggccgttgg gtaggagaga tgctgcttca tatcaccgac tccaagactc
    24721 ggatccggga ttcgctggcg acccactgcc acttgtggga accatcggct tttagtctac
    24781 agtgatgcag ctatttggtc aacaaaaacc cgtccgtgtg cgtaagggat ctatcggcct
    24841 gccgagtcaa gcgcggttgg cccgcgcgta tgcgggttgg gggcctgctg gaccgagatt
    24901 cccatctcgg tcctcggttg gcccgcgcgt atgcgggttg gggtcggccg atcgagattc
    24961 tggcctggat tgactgaagg agtcttaccg tcaaagagtt atgacaagtc agccctagcc
    25021 ttgcgcgggc ggttcctggg ggcggatctg tgaaggatgc ttatcggccc tcggcaggct
    25081 ctgatgcctt tctggctcgc tc
//
LOCUS       DAOTPW010000056        24963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2079_length_24963_cov_5.098001, whole genome shotgun sequence.
ACCESSION   DAOTPW010000056 DAOTPW010000000
VERSION     DAOTPW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 24963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24963
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2079_length_24963_cov_5.098001"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="P5316_07020"
     CDS             complement(<1..1134)
                     /locus_tag="P5316_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84746.1"
                     /translation="MAIDPRHLKPAQLVQLLNSWGEVISERQLLRHRSRAGLRIGDGR
                     TIDLIRYVAWLVQERHAPRPEPDGPTGYEALKERARQRNLALSLAGRDIGELPPVADP
                     PRKAKAARDFRFFCEQYFPQTFHLPWSPDHLKVIAKIELAVLAGGLFAMAMPRGSGKT
                     SLCETACLWAMVYGHREFVALIGADEEHAANMLDSIKAELENNERLLADFPEVVFPIH
                     RLEGIHQRSAGQLYQGKQTHIGWTAREIVLPTIPGSAASGAIIRVAGITGRIRGMKHK
                     RADGSSVRPSLVLIDDPQTDESARSPSQCATRERILAGAILGLAGPGKKIAGLMTLTV
                     VRPDDMADRILDREKHPQWQGERTKMVYAWPTNEALWARYTEIW"
     gene            complement(1124..1330)
                     /locus_tag="P5316_07025"
     CDS             complement(1124..1330)
                     /locus_tag="P5316_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84747.1"
                     /translation="MGEPARMNPTAIAVADAARLLSAAGGRSVTPEQIEADIEAGMPT
                     NADGTINLVRYAAWLVKEMSAGGD"
     gene            1490..1732
                     /locus_tag="P5316_07030"
     CDS             1490..1732
                     /locus_tag="P5316_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84748.1"
                     /translation="MTTNANNKTAAETYATRRHEVARLMDVLEMELKKYDDQAAAKPK
                     DWGFAGTMAHVRECLIDLVEGLSGIERSEIERFLSE"
     gene            1752..1979
                     /locus_tag="P5316_07035"
     CDS             1752..1979
                     /locus_tag="P5316_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84749.1"
                     /translation="MTTKDMSDLLEGLFNEIAGCEDDDEMTPAELDPDGTLYECQASS
                     FRDCGLLTSNDGVVLTLRDGTEFQITIVRSR"
     gene            2036..2590
                     /locus_tag="P5316_07040"
     CDS             2036..2590
                     /locus_tag="P5316_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRR84750.1"
                     /translation="MKKDQIQIGGLYTAKVSDKVVTVRIDSVNSHGGWNATNTATGKR
                     IRVKSAQRLRAEAKGGKKKAAEAPKPDVTPTVEIVTGQPVPEPAKREKKTRKTAADKP
                     KRVSALDAAAEVLKAEGKPMRAKDLIDAMVAQGLWSSPNGKTPEATLYAAMIREIRKA
                     VETGTVSRFRKIDRGQFEYNEHYA"
     gene            complement(2581..3951)
                     /locus_tag="P5316_07045"
     CDS             complement(2581..3951)
                     /locus_tag="P5316_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HRR84751.1"
                     /translation="MEITLRPLADIKPYEKNPRINDAAVDAVAESIRRFGFRQPIVVD
                     TDGVIVCGHTRWKAAQKLGLAEVPVHVARDLTPEQIRAYRIADNKTAELAEWNMELLP
                     IELAELQGAGIDWSLLGFDADDLAKLLDPGVQQGLTDPDEVPEPPDEATTQSGDIWLL
                     GNHRLMCGDSSRPEDVDHLLDGQRIHLVNTDPPYNVKVEPRSNNAIAAGLSSFGEAGV
                     THHQGFDLARRPEIARPTHHKLRPKDRPLANDFVSDEEFDRLLDAWFGNMARVLEPGR
                     AFYIWSGYANCANYPPVLKRCELYFSQAIIWVKEHPVLTRKDFMGNHEWCFYGWREGA
                     AHVFLGPNNAVDVWSVKKVNPQSMIHLTEKPVELAVRAMQYSSRAGENVLDLFGGSGS
                     TLIAAEQTGRRAYLMELDPLYCDVIVVRWEKFTGRKAERISGRSNAPVEAEASEEKAS
                     CVTDQA"
     gene            complement(3966..4550)
                     /locus_tag="P5316_07050"
     CDS             complement(3966..4550)
                     /locus_tag="P5316_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84752.1"
                     /translation="MAKVELIRTLRAPEMWKSIPRASQGMTWNPADLTYDRLATVQRL
                     LDVRDVQRMLLWFWIKGITADATADIQIAGWPLFGDVNTPVVPGNQGSIAKPHGWIMA
                     DGQLAFDYHSPIAKNPITGVAETWFPAHNWTPSPDYNTHFYMSASGAVANPTAGKAQP
                     LIFEIGDLKNIGYVDIQADVLPTASTLLIIAASS"
     gene            complement(4906..5169)
                     /locus_tag="P5316_07055"
     CDS             complement(4906..5169)
                     /locus_tag="P5316_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84753.1"
                     /translation="MAKHRPKPRNGFEDSLPVMADEWNRLPAEVEQAYRRGFQQGLAV
                     AIEGLRSGATADDIEEFTYGIVHAWRYQDPMRWSPAPRMEDQG"
     gene            complement(5458..7314)
                     /locus_tag="P5316_07060"
     CDS             complement(5458..7314)
                     /locus_tag="P5316_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HRR84754.1"
                     /translation="MDCDSAMLHAALWYAELGYPVFPCVPGGKTPLPAHGLLEATTDA
                     ERITGWWMRHPDANVAIRTDDLLAIDVDGRDNPWLADEPAQRADLEIAPQSLTPRGGR
                     HYFFRQPEGRSWRNTAGRLAPHVDSRAHGGYVLVPPSTVGGGSYCWRDGHELTVSPAR
                     LPEPPAWLTGQLDALTAANGTVQPDTPANAIPRGHRNATLARLAGAMRRVGMSQAEIG
                     AALRQANADRCAPPLPAREVERIAASIARYEPDGVAVALVENHWDQDRDVPVRNQPLT
                     VRELMAAYRMLRAPVLQGLLRRGETMNVIAPPKTGKSWLVLALAMAIATGRLWLDTFE
                     TVRGDVLILDNELHPETLAHRIPQVAECLRIGMNEIAETMSVQSLRGQLRDIFSLGPY
                     FESVAPGRFALVVLDAFYRFMPRDMDENDNGTMANIYNHLDALADRLGCAFVLIHHAT
                     KGNQSAKAVTDVGAGAGSQSRAADAHLVLRPHEEPGAVVLEAAVRSWPPVEPRVLRWS
                     FPLWRPAPDLDPARLKSEKPKRAKADAKESSEPPAWNVERFVETFLSDQPATKAEIRA
                     RAADVPGLSWRRIADLLDIAEVRGLIHRRRVGKAHQVLYATVPPSTEQEVRS"
     gene            complement(7329..9002)
                     /locus_tag="P5316_07065"
     CDS             complement(7329..9002)
                     /locus_tag="P5316_07065"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRR84755.1"
                     /translation="MELRAYQREAVEAVYTHLRGRDDNPCVVIPTGGGKTAVMATICR
                     DAVQMWDGRVLILAHVKELLEQIAEKLRTMAPDLPFGIYSAGLKRRDLGYAVTIAGIQ
                     SVYQRACDIGPVDLAIVDEAHLIPPDGEGMYRQFLADAKVINPQMRVIGLTATPFRMK
                     SGMICAPENILNAICYEIGVRELIVQGYLCPLRTKSGSQRPDYDQLHVRGGEFIANEV
                     EDLMDDDALVRSACREIVEHAADRKSVLIFASGVRHGEHIVRVLRERHGAECGFVCGE
                     TLPFERAQTLRRFRDGSLKYLCNVNVLTTGFDAPNIDCIALVRPTLSPGLYYQCIGRG
                     FRLHEGKTDCLVLDFGGNVLRHGPVDQIRIEAPREGQGHTPAKECPQCHEVIAAGYGM
                     CPQCGYEFPPPERAKHDAEASSESILSDPASRTEHEVASVFYAVHTKRDAPPDAPRTM
                     RVDYRIGFNQYVSEWVCFEHTGYARQKAESWWRARSVESVPNDAAEAVELAHAGALAR
                     TLAVTVEHKSGEKYDRVVSHRLGDKPPRLESEEGLPEYAPLGDEDSIPF"
     gene            complement(8977..9354)
                     /locus_tag="P5316_07070"
     CDS             complement(8977..9354)
                     /locus_tag="P5316_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HRR84756.1"
                     /translation="MTRTYELPYPPSVNHYWRRVGWRTLISREGRRYRKEVVALLAAQ
                     RAQPLGGRLVMRVTVFPPDARRRDLDNVQKALLDALQQGEAFHDDSQIDELVIKRGAV
                     VPDGKTIVEITEIDANGTASVPA"
     gene            complement(9351..9809)
                     /locus_tag="P5316_07075"
     CDS             complement(9351..9809)
                     /locus_tag="P5316_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HRR84757.1"
                     /translation="MANLNGFDAREVDPSVGFDPIPAGKYLAIISGSQMKPTKSGAGN
                     YLELTFQILEGEFKGRLVWTRLNLDNPKAETVKYARAELSAICRAVGVMAPRDSVELH
                     DLPLIITVAHKKRSDTDEITNVIKGYAKKDGNGTRPAAPAGNNGKAPWQK"
     gene            complement(9813..10595)
                     /locus_tag="P5316_07080"
     CDS             complement(9813..10595)
                     /locus_tag="P5316_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR84758.1"
                     /translation="MSLMNQIQRGKAEMPRRVLVYGTQGIGKSTFGSMADRPVFIQTE
                     DGLGGIDCERFPLASKYGDVLSALAELYTAPHEFGTVVLDSLDWLERLIWAEVCRARG
                     VESIEDIGYQKGYTFALTQWREVLEGLNALRTDRGMQAILIAHAKIERFENPETDAYD
                     RYVPRLHKLASALVQEWCDEVLFATYRVHTRKVGEQFGQDRHKGIGTGERIIRTTERP
                     AHVAKNRLSLPDEIPLDYRIYAAFVRGENPLAMSEQPAEQGA"
     gene            complement(10718..11614)
                     /locus_tag="P5316_07085"
     CDS             complement(10718..11614)
                     /locus_tag="P5316_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HRR84759.1"
                     /translation="MRTNKCLPLVRIGQGTSQRDIYPEEIMTPKNGIDLRFLIREPAE
                     VYHAKAKDYLSAHALADFRRCPLLYRKKQLGLIPDHDSTFYLIGRAAHTLILEGRERY
                     AREFAVGGPINPKTGQPFGSNTKAFAEWAAQQSKAVLSDDQAALVEQMAAAVKGHELA
                     RELLADGSAEGVVRCEYEGHACQARIDWISADRDQGIVDLKTADTIDSFELSASAFGY
                     MHQLAFYRALVARVSGRILPVHLIAVEKREPYRCGVWRISPAVLDAAQEQNESDLQQL
                     SRCRATGIWPTRYEALRTIDRL"
     gene            complement(11611..11982)
                     /locus_tag="P5316_07090"
     CDS             complement(11611..11982)
                     /locus_tag="P5316_07090"
                     /inference="COORDINATES: protein motif:HMM:NF019248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1580 domain-containing protein"
                     /protein_id="HRR84760.1"
                     /translation="MHGQTITDAHITLTEAANIAPGRPSANCIWRWCRRGVRSRNGDR
                     VRLQHMRIGGKIFTTARWLEEFGRALADADARYFDLCEAASHAAAAGPRRRRLRSPSA
                     FEEQRRREIAEAERELEEAGL"
     gene            complement(12217..13560)
                     /locus_tag="P5316_07095"
     CDS             complement(12217..13560)
                     /locus_tag="P5316_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRR84761.1"
                     /translation="MPTKVPSYRKRKGYSQALVTLTDAATRRRRDYWLGEHGTPESRE
                     AYHRIIAQWEAAGRRLPANDSAGACSATTTSVTITELVRDYWRWAEEYYRPKHTQALI
                     CALRLMRQYYGQTLAAEFGPKKLRLLREEMIRGDEGAVPPRRAWARKFINSQVQRIRH
                     MFKWAVAREMVPASVYQALCTLEPLKRGRTTAREGRKVGPVSQPLLDATLPLLSRPVR
                     ALVDLQLLTGARPGELLELRACDLEIDEKAGIWTYRPERHKNVHREHDRIIYFGPRAQ
                     AVIRPFLGNRPTTAFLFSPADAEAERREALHAARTTPLSCGNRPGTNRRDEPACSPGD
                     RYTTNTYAQAIRYACDRAYPPPLPFGREKKETKKAWRERLKKKPELKEQLLEWQRAHR
                     WHPHQLRHNAATLLRREFGLEAAQLALGHASAQITDAVYAERDHAKVVEIMRKIG"
     gene            complement(13678..13749)
                     /locus_tag="P5316_07100"
     tRNA            complement(13678..13749)
                     /locus_tag="P5316_07100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13715..13717),aa:Gly,seq:ccc)
     gene            14830..16590
                     /locus_tag="P5316_07105"
     CDS             14830..16590
                     /locus_tag="P5316_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84762.1"
                     /translation="MASYPQTYQIARQGAVRPVTVGNDEDVIALRTELELMSSFAQGA
                     TPELVEALEDRLAAVKSGAAEEGGRTAEDIKDMVAILRDAMHPSTGEAIAKLDADFPT
                     ARGEIDMAAVEKESNDFHEDVGNEVTASGKPSAGRVGQAFKQAMLNKAVAARRAAPET
                     SGVSETPASENASETGAASGQQPASAEPASGQQVPAESSAGTTESDPVEAALREAMGI
                     AEGALAGETSDMSGVGDEFDEEGLSVEDLEAEGVTEGEKADEVEGMTEGDSLTGKAGV
                     AEGTAGGEATVDPLEQIMAEQAGLPDSEVSLDQLADMLLAGQANPSPAGQESVTEGSG
                     IDATDAVDASRSIPENGGRAQEVTDARDQTEVDKADASQNTACVPKDEAGQPDLAAEE
                     VAQAIEASRTEIDAIASAFEEATADLGALADQVCDSAIPPGDSVLPEAELIGAAEIPE
                     AAQGMELSADEVAEISSDLNAAGMEDEPAFQTSNLPSLNMREELRTVRETIQTGLERL
                     IQLLDHMDRTHIEAENRLARAKMYQQAARRAQEASEKLVQARTEAADARAAYESAQRR
                     LEEAESAWEEARRAADDAAL"
     gene            complement(16836..19349)
                     /locus_tag="P5316_07110"
     CDS             complement(16836..19349)
                     /locus_tag="P5316_07110"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRR84763.1"
                     /translation="MHSLNKDSRTPLKRTLHHPSRFALFLLITLAIPACTTTKVVMHD
                     RLAADGSNSFYIGNRAPLLPGPLIKLPVGAVEPKGWLRSQLQFQAEGMVGHLAEISQW
                     CNFAESAWVTPDGTGKWPWEEMPYWLKGYVDLGLLLKDPRIMAEATKWIRAILDSQRP
                     DGTFGPKDNFVNNDLWPHMCILYAMRSYYEATGDPKVISVMTNYFRYVQKIPVDKLYT
                     WGKQYGAGWWQWIRAGDHLDSIYWLYNQTGEPWLLDLAKVNHERTADWTGGIASWHGV
                     NIAECFREPAQYYVQSHDPKHFAAAARNYDEVRAKFGQVPGGMYGADENCREGFHDPR
                     QGTETCAFVEMMHSHEILLKISGDGLWADRCEEVAFNSFPPSLTPDLKGLHYLTCPNQ
                     IQCDRADKTPMIENRGDMMSYTPYEQYRCCQHNVAFGWPYFVEHLWMATPGNGLAAAM
                     YAPSKVTARVGDGTKVTIDEKTDYPFDDVIDLHFSMAQSVEFPLSLRIPGWCKLAHIR
                     INDQAQEPVSSRWVVYTRKWSDGDKVRIELPMEISTHVWTANRNSVSVSRGPLTYSLK
                     IGEKWQEYDNGKPWAAYEVFPATPWNYGLIVNPGDPATSFEFKRTGVRVGQQPFRPEA
                     APVMLKAKGKRIPEWQQEKNGLVGEVPESPVRSDQPVEELTLIPMGCARLRIAAFPRI
                     GDGPDAKTWKKPPPKPALTASHSNPADSLQAVADGVIPKSSSDQSIRRFTWWPHRGTN
                     EWVERALYEPQTIGYCEVYWFDDGGTGSCRVPKSWTIEYRPKSGGPWTSVKNTGPYGV
                     EKDTFNKVSFEPVDAIEIRIKVELQPGFSGGILEWRIGE"
     gene            19641..21362
                     /locus_tag="P5316_07115"
     CDS             19641..21362
                     /locus_tag="P5316_07115"
                     /inference="COORDINATES: protein motif:HMM:NF040163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5722 domain-containing protein"
                     /protein_id="HRR84764.1"
                     /translation="MNPDLGTVTFLSFAMLFCAGAAPVQKSREKLGRGSLPAESSAQE
                     SQVQPASKTEAPVVKATSGQIQIVLPPGEHAGKVVVYELRPNEKDDAWRGKKPIETAG
                     MAARRMVKLNVDRFERTPGGRRDRLYSKFVVVPVNGQEPIMPARFVEDFTSLQRRRLP
                     FPRTRSKKGLQVQMVDDAIALGVQHAALNVDLARLFAWTPMPDAIPHVVDGERFLLNP
                     SLVVDLDRRVTMLSQAGMTVTLILLNYANTAEAGAAEVLRHPNYNDGCPNQISAFNTA
                     TADGLRHYRAAVEFLIDRYTQPDGRFGQALGYIIGNEVNSHWHWCNMGEVSMEEFTED
                     YLRAMRIAATAAEKICPTARVYMSLEHHWNARFDSQTKSFPGRAFLELFARRAREQGD
                     FAWHLAYHPYPDNLTEPAFWRDRAATTDVNTTPKITLKNIELLPEYLRRPELLYKGEP
                     RRLILSEQGFHTKAGPDGEILQAAAFCAAFVKVAHIDGIDAFILHRHVDYRDEGGLKL
                     GLWTRNEASPGPGDPGRPKRIHEVFRMADTPGWAAAFEFAKPIIGIRDWAELLPPSAG
                     QPLPSRK"
     gene            complement(21454..23676)
                     /locus_tag="P5316_07120"
     CDS             complement(21454..23676)
                     /locus_tag="P5316_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84765.1"
                     /translation="MTIGDFPPRDDRYTILQGEMDWLRIPVWESLFSQSNGLLGIRGS
                     FEEPMIGTRSRNLTLMAGLYDTLPDGLPELPPLPDALTTHIVLCDQPFDLRRGHIRSF
                     SRWLDMKRGLLCREVTWQDAAGHTTRLTFQRFVSLANRNILAVRIRIVPVDWSGPVEL
                     KTGIRHSEPAAFGKVSHWDPPKLTSSAHGLATLETRTSQSRRPLAVAGAYVAVDQQNP
                     IPGEASSDPCTAGQSYILDVSEDAVYCFDRFVAFMAGSPDAESPAKAARNAALSAAKS
                     GWNEQLEDHIVACAELWDQMDVEIDGPVEDQQAIRFNLFQLATLCPPPGEIAGIAPKG
                     LGGTHYGGHVFWDTEIYMLPFFALTNPQGAKTLLTYRRATLDGARRKARSNNYRGAQY
                     AWESADTGDETCPASWTDPTTGEKHRIWCGDIQDHISADVSFAIDQYVRVSGDEDFLW
                     EHGAEVMFETARFWASRVTRNAAGRFEIRDAMGPDEFHIHVDNDAFTNYMARWNLQAA
                     ADLWDNAAFPKSKRRDLAAQLGLDSDEVATWRDIAKNIVLLCDEQTGLINQHAGFFDR
                     PEVSPDILRITRSTSITEIIGAGMAIESQVLKQAEVVLLQTLLEDQFSRESREANYRY
                     YEPRTSHDSSLSVSAHAWAAASLDHIEEAYSYFRRAAYLDLNDLAGNTAEGLHTANMG
                     GVWLTVAMGFAGLNLRGESPTATPALPANWRHLKMNISFRGKRFRVECSSDRGAVEPR
                     "
     gene            complement(23802..24668)
                     /locus_tag="P5316_07125"
     CDS             complement(23802..24668)
                     /locus_tag="P5316_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR84766.1"
                     /translation="MKLSRRQWLAGAGTAAAGLALTRLQAMADETRKSGMKFGMCDWS
                     IGRDDPGVFDVAKQIGLDGVEVSIGYPKDGLQLRRAEVQKAYMEAAHRTGLSIPSTAM
                     GVLNDVPLVSEPKAALWVADGIEATARLGAKSMLLAFFGKGELKEDAKEDLRRVTEAL
                     VELAPRAEKAGVILGFESYLSARTLIGILDQVKSPALQVYYDIYNNAHVKHDPLEEMR
                     LLGRNKICQIHFKDYPMLEKGSGKVDWPATVRVLREIKYDGWIVLETGSPNKDIVADT
                     KKNLAYVKGLFA"
     gene            complement(24749..>24963)
                     /locus_tag="P5316_07130"
     CDS             complement(24749..>24963)
                     /locus_tag="P5316_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR84767.1"
                     /translation="STGTAFCYNVARVLTAVIILLGGGLQLALKKAGVDEVFRWGAVI
                     LCGFYFLGIIALIWAPETKDKPLPED"
BASE COUNT         4213 a         7666 c         8457 g         4627 t
ORIGIN      
        1 ccagatctcg gtgtaccgtg cccacagggc ctcgttcgtc ggccaggcgt agaccatctt
       61 cgtccgttcg ccttgccact gcgggtgctt ctcgcggtcg aggatgcgat cggccatgtc
      121 gtccggacgc acgacagtca gcgtcatcag cccggcgatc ttcttaccgg gaccggccag
      181 gccgaggatg gcgccggcca ggatgcgttc gcgcgtggcg cactgactcg ggctacgtgc
      241 cgattcatcc gtctgcgggt cgtcgatcag cacgagcgac ggccggacac tcgatccgtc
      301 cgcccgcttg tgcttcatgc cgcgaatccg accggtgatc cctgccacgc ggatgatggc
      361 cccggacgcc gcgctgccgg ggatcgtggg cagcacgatc tcgcgggcgg tccagccgat
      421 gtgcgtctgc ttgccctggt agagctggcc ggccgaccgc tggtggatgc cttcgagcct
      481 gtggatcggg aagacgacct cggggaagtc ggcgagcagt cgctcgttgt tctccaactc
      541 ggccttgatc gagtcgagca tgttggcggc gtgctcctca tcggcgccga tgagggcgac
      601 gaactcgcga tggccgtaga ccatcgccca caggcaggcc gtctcgcaca ggctggtctt
      661 gcccgatccc cggggcatgg ccatcgcgaa aaggccgccg gcaagaaccg ccagttcgat
      721 cttggcgatg accttcaggt gatccgggga ccacggcagg tgaaacgttt gcgggaagta
      781 ctgctcgcag aagaaccgga agtccctcgc ggccttggcc tttcgcgggg gatcggccac
      841 gggcggcagc tcgccgatgt cgcggccggc gagcgacagc gcgaggttgc gttgccgcgc
      901 ccgttccttc agggcctcgt agccggtggg accatcgggc tccggccgcg gcgcgtggcg
      961 ctcctggacg agccaggcga cgtagcggat cagatcgatg gtccggccgt cgccgatgcg
     1021 aagccccgcg cggctgcggt ggcgcagcag ctgccgttcg ctgatcacct cgccccagga
     1081 gttcagcagt tggaccagtt gcgccggttt cagatgcctc gggtcaatcg ccaccggcgg
     1141 acatctcctt cacgagccat gcggcatagc gcacgaggtt gatcgtcccg tccgcgttcg
     1201 tgggcatccc ggcctcgatg tcggcctcga tctgttccgg tgtcaccgaa cggccacctg
     1261 cggccgacaa aaggcgggcg gcgtccgcca cggcgatcgc ggtcgggttc atgcgcgcgg
     1321 gctcgcccat tcacgccgcc tcctgccgac atgaagttcg gtcacaccgc atgtcggtac
     1381 cggtgagtac ggtgcagccg aatcttcgag aaatcgcaga ttctccgggg aattgccttc
     1441 cctttcgcga aacatgaggt aagtcatgca caacgaaagg agacgaacga tgacgacgaa
     1501 cgccaacaac aagactgccg ccgagacgta tgccacgcga cgccacgagg tcgctcggct
     1561 gatggatgtc ctggagatgg aactgaagaa gtacgacgac caggccgccg ccaagccgaa
     1621 ggactggggc tttgcgggca cgatggcgca cgtccgtgaa tgcctgatcg acctggtcga
     1681 ggggctctcg gggatcgagc gcagcgagat cgagaggttt ctgagcgagt gatcgcagaa
     1741 aggaaacgac catgaccacg aaagacatga gcgatttgct ggaaggcctg ttcaacgaga
     1801 tcgccggctg tgaggacgac gacgagatga cgccggccga gttggacccg gacgggacgc
     1861 tgtacgaatg ccaggcctcg tccttccggg actgcgggct gctgacgtcc aacgacggcg
     1921 tggtcctgac gcttcgcgac gggaccgagt tccagatcac gatcgtgcga tcgcggtagg
     1981 ggattggaac gccaggggcc cggccagccc gaacacggcc agaggagaca atgccatgaa
     2041 gaaggaccag attcagatcg gcggcctgta cacggccaag gtgagcgata aggttgtcac
     2101 ggtgcggatc gacagcgtca acagccacgg cggctggaac gcgacgaaca ccgccacggg
     2161 caaacgcatc cgcgtcaaga gcgcccagcg cctgcgggcc gaggccaagg gcggcaagaa
     2221 gaaggccgcc gaggcgccca agcccgacgt aacgcccacc gtcgagatcg tcaccggcca
     2281 gccggtgccg gagcccgcca agcgcgagaa gaagacccgc aagacggccg ccgacaagcc
     2341 caagcgggtc agcgccctgg acgccgccgc cgaggtgctc aaggcggaag gcaagccgat
     2401 gcgggccaag gacctgatcg acgcgatggt cgcccagggc ctgtggtcca gccccaacgg
     2461 caagacgccc gaggccacac tgtacgccgc catgatccga gagatccgca aggcggtcga
     2521 gacgggcacc gtctcccggt tccgcaagat cgatcgcggc cagttcgagt acaacgagca
     2581 ttacgcctga tcggttacac atgacgcctt ctcctccgac gcctcggcct cgaccggggc
     2641 gttgcttcgg cctgaaatcc tctccgcctt gcgtcccgta aacttctccc acctgacaac
     2701 gatgacatcg cagtacagcg gatcgagttc catcaagtac gcgcgccggc ccgtctgctc
     2761 agcagcgatc agcgtgctgc ccgagccgcc gaacagatcc agtacgttct cgccagcccg
     2821 cgatgagtac tgcatggccc gcaccgccag ctcgacgggc ttctccgtca aatggatcat
     2881 gctctgcggg ttgaccttct tgaccgacca gacgtccacg gcgttgttcg gaccgaggaa
     2941 gacatgggcc gcgccttccc gccagccgta gaaacaccac tcgtggttgc ccatgaagtc
     3001 cttgcgggtc aggaccggat gctccttcac ccagatgatc gcctgcgaga agtagagctc
     3061 gcagcgcttg agcaccggcg ggtagttcgc gcaattggca tagccgctcc agatgtagaa
     3121 ggcccgcccg ggctcgagca cccgggccat gttcccgaac cacgcatcca gcagccgatc
     3181 gaactcctcg tcgctgacga agtcattggc cagcggacga tccttgggcc gcagcttgtg
     3241 gtgcgtgggt ctggcgatct caggacgccg cgccagatca aaaccctgat ggtgcgtgac
     3301 gcctgcttcg ccgaacgacg acaggccagc cgcgatcgcg ttgttgctcc ggggctccac
     3361 cttcacgttg tacggcggat cggtattgac cagatgaatc ctctgtccat ccaacagatg
     3421 gtccacatcc tcgggccgcg acgagtcgcc gcacatcaga cggtggttgc ccaacagcca
     3481 gatgtcacct gactgtgtgg tcgcctcgtc tggtggctcg ggtacctcgt cgggatcggt
     3541 cagcccctgt tggacgcccg ggtcgagcag cttggccagg tcgtcggcgt cgaatccgag
     3601 caacgaccag tcgatgccag cgccctggag ctcggccaac tcgatcggca gcaactccat
     3661 gttccactcg gccaactcgg ccgtcttgtt gtccgcgatc ctgtacgccc ggatctgttc
     3721 gggcgtcagg tcgcgggcga cgtgaacggg cacctcggcg agcccgagct tctgcgccgc
     3781 cttccatcgc gtgtgcccgc agacaatcac gccgtcggta tccaccacga tcggttggcg
     3841 gaacccgaag cggcggatgg actcggcgac ggcatcgacc gccgcgtcgt tgatccgggg
     3901 gttcttctcg tacggcttga tgtcggccaa cggccgaagg gtgatttcca tcgatgacct
     3961 ccgtgtcacg aagatgccgc gatgatcaac aacgtcgaag ccgtcggcag gacgtccgcc
     4021 tggatgtcca catacccgat gttcttgagg tccccgatct cgaagatcag cggctgtgcc
     4081 ttgcccgccg tcggattggc gaccgcgccg gacgcggaca tgtagaagtg cgtgttgtag
     4141 tccggcgatg gcgtccagtt gtgggccggg aaccaggttt ccgcgacgcc ggtgatgggg
     4201 ttcttggcga tcgggctgtg gtagtcgaag gcgagctggc cgtcggccat gatccagccg
     4261 tgcggcttgg cgatgctgcc ttggttgccg ggcacgaccg gggtgttcac atcgccgaac
     4321 aacggccagc cggcgatctg gatgtcggcc gtggcatccg ccgtgatgcc cttgatccag
     4381 aaccacagca gcatccgttg gacatctcgc acatcgagca gtcgctggac cgtcgcgagc
     4441 cgatcgtagg tcagatcagc ggggttccac gtcatgccct ggctcgccct cgggatggac
     4501 ttccacatct ccggggcccg cagcgttcga atgagttcga ccttggccat cgcaatgcct
     4561 ccttggcttt caccgccgac cggcgctgtg ccgccggcgc ggtcatccgt gacctatccg
     4621 ttcacctcgg ggccgcccgg cgtcgccaac gtggcgacgg ggcgccccag gggtaccgac
     4681 cgtacgcccg ggcgacgacg atcgcccaga gtggccccca acgcgtcccg tggccgccct
     4741 gggtgccgcc cggcggcggt cggaccggca aaacaaattc ggttggggtt gggagctgtt
     4801 cccggcggcc aaacccgccc cgttttcgcg gaagtaccta tgcattcgtt tcacgcgttg
     4861 caccgccttg gcttcttgac gcgtcgccga gcggaatcca cctattcatc cctggtcctc
     4921 catccgcggc gctggcgacc agcgcatggg gtcctgatat cgccacgcgt gaacaatccc
     4981 gtatgtgaac tcctcgatgt cgtcagccgt cgcgccgctg cgaagcccct cgatcgctac
     5041 ggccagaccc tgctgaaacc ctcggcggta ggcttgctcg acttccgcgg gcagccggtt
     5101 ccattcgtcc gccatcacgg gcaggctgtc ctcgaaccca ttgcggggct tgggtctatg
     5161 ctttgccatc tggattcctc tcggtaacga gatcaggctc atcgtgtgcg cgcgcacgcg
     5221 cgcgcccata catgcccggg atgtgctcat aagggttcat cagtttcgct ggagcgcttc
     5281 gccgaagcgc gcgcgctcca gcgaccccag ggggcgtcgc tcgagcgcac gcccctgggg
     5341 ggtgtggggg gtgtgcgcgc gcacgagcga agtcggcttc gacgggttcg gaagttcgtc
     5401 ggagcgcttc ggcgaagcgc tccagcgatg cgcgcacgag cgaacgggga gccggtttca
     5461 cgaacggacc tcctgttctg tggacggcgg gacggtcgca taaagcacct gatgcgcctt
     5521 gcccacccgc cggcggtgga tcaacccacg gacctcggcg atgtccagca ggtccgcgat
     5581 ccgcctccag gacagcccgg ggacatccgc cgcccgcgcg cggatctccg ccttggtcgc
     5641 cggctggtcc gacaggaacg tctcgacgaa ccgttcgacg ttccatgccg ggggctccga
     5701 ggattccttc gcatctgcct tcgcccgctt cggcttctcg ctcttgagcc tcgcgggatc
     5761 gaggtcgggg gcgggcctcc acagcgggaa cgaccaccgc aagactctgg gctcgaccgg
     5821 cggccacgac cggacggcgg cctccagcac gaccgccccg ggctcttcgt gcggccggag
     5881 cacgaggtgg gcgtccgccg cccggctctg gctgccggcg ccggccccga cgtcggtgac
     5941 ggccttggcc gactggttgc ccttggtcgc gtggtggatc agcacgaacg cgcagcccag
     6001 ccgatcggcc agggcgtcaa ggtggttgta gatgttggcc atcgtgccgt tgtcgttctc
     6061 gtccatgtcg cgcggcatga agcgatagaa ggcgtcgagc acgaccaagg cgaagcggcc
     6121 gggggcgacc gactcgaagt acgggcccag cgagaagatg tcgcggagct ggccgcgcag
     6181 gctttgcacg ctcatcgtct cggcgatctc gttcatcccg atccgcaggc actcggcgac
     6241 ctgcgggatg cggtgggcga gcgtctcggg gtgcagctcg ttgtcgagga tcagcacgtc
     6301 gccccggacc gtttcgaacg tgtcgagcca aaggcgcccc gtggcgatcg ccatcgccag
     6361 cgccagaacc agccagctct tcccggtctt gggcggcgcg atgacattca tcgtctcgcc
     6421 acggcgcagc aggccctgga ggaccggggc acgcaacatc cggtacgcgg ccatcagttc
     6481 ccgcacggtc agcggctggt tcctgaccgg cacgtcgcga tcctggtccc agtggttctc
     6541 gaccagcgcg acggcgacgc cgtccggctc gtagcgggcg atgctcgccg cgatccgctc
     6601 gacctcacgt gccggcagcg ggggcgcgca gcgatcggca ttggcctgcc gcagggcggc
     6661 gccgatctcg gcctgcgaca tgcccacgcg gcgcatcgcg ccggccagcc gggccagcgt
     6721 ggcattgcga tggccccggg ggatcgcatt cgccggtgta tcgggttgaa ccgtgccgtt
     6781 ggccgcggtc agggcgtcga gctgcccggt cagccaggcc ggcggctcgg gcagccgggc
     6841 cggcgagacg gtcagttcgt ggccgtcgcg ccagcaatac gacccgcctc ccacggtaga
     6901 cggcggtacc agcacgtagc cgccgtgggc gcgcgagtcg acgtgcgggg cgagccggcc
     6961 ggccgtgttc cgccacgaac ggccctcggg ctgccggaag aagtagtgcc gtccgccccg
     7021 tggcgtcagc gactgcgggg cgatctcgag gtcagcccgt tgggccggct catcggcgag
     7081 ccaggggttg tcccgcccgt ccacgtcgat cgcgagcagg tcgtcagtgc ggatggcgac
     7141 gtttgcatca gggtggcgca tccaccagcc cgtgatccgt tcggcatcgg tggtggcttc
     7201 cagcaggcca tgcgcgggaa gcggcgtctt gccgccggga acgcagggga acaccggata
     7261 acccaattcg gcgtaccata gcgccgcatg gagcatcgcg ctgtcgcaat ccattgccgg
     7321 cacactcctc agaacggaat cgagtcctca tcgcccaggg gggcgtactc cggcaggccc
     7381 tcctccgatt cgagccttgg cggcttgtcg cccagcctgt ggctgacgac gcggtcgtac
     7441 ttctcgccgg acttgtgctc gacggtgacg gccagcgtcc tggccagggc gccggcgtgg
     7501 gccagctcga cggcctcggc ggcgtcattc ggtaccgact cgacggagcg ggcccgccac
     7561 caggattccg ccttctggcg ggcgtagccg gtgtgctcga agcagaccca ttccgatacg
     7621 tactggttga agccgatgcg gtagtcgacg cgcatcgtgc gtggcgcatc cggcggtgca
     7681 tcccgtttgg tgtgaacggc gtagaagacg ctcgcgactt catgctcggt gcggctcgcc
     7741 gggtcggaca ggatgctctc gctgctggcc tccgcatcgt gcttggcccg ctccggcggt
     7801 gggaactcgt acccgcactg cgggcacatg ccgtagccgg ccgcgatcac ctcgtggcac
     7861 tgcggacact ccttggccgg cgtgtggccc tggccctccc ggggcgcctc gatgcggatc
     7921 tgatcgaccg gcccgtggcg cagaacgttg ccgccgaagt ccagcacgag gcagtcggtc
     7981 ttgccttcgt gcaggcggaa tcctcgccct atgcactgat agtagagtcc cggcgagagc
     8041 gtcgggcgga ccagggcgat gcagtcgatg ttcggggcgt cgaagccggt cgtcagcacg
     8101 ttgacgttgc ataggtattt gaggcttccg tcgcggaacc gccgcaacgt ctgggcccgt
     8161 tcaaacggca gcgtctcgcc gcagacgaac ccgcattcgg cgccgtggcg ttcccgcagg
     8221 acgcgcacga tatgctcgcc gtgccggacg cccgaggcga agatcagcac ggactttcgg
     8281 tcagccgcgt gctccacgat ctcgcgacag gccgaacgaa cgagggcgtc atcgtccatg
     8341 aggtcctcga cctcgttggc gatgaactcg ccgccgcgaa catgaagttg gtcgtaatcg
     8401 ggccgctgcg agcccgactt ggttcgcagg gggcagaggt agccctgcac gatcagctcg
     8461 cgaacgccga tctcgtagca gatggcgttg aggatgttct cgggcgcgca gatcatgccc
     8521 gacttcatgc ggaatggggt cgccgttagc ccgatcaccc gcatctgcgg gttgatgacc
     8581 ttcgcgtcgg ccaggaactg ccggtacatc ccctcgccgt cgggcggaat gagatgggct
     8641 tcatcgacga tcgccaggtc caccggcccg atgtcgcagg cccgctggta gaccgactgg
     8701 atgccggcga tcgtcacggc gtagccgagg tcccgacgtt tcaggccggc ggagtagatc
     8761 ccgaacggca ggtccggcgc catcgtgcga agcttctcgg cgatctgctc gagcagctcc
     8821 ttgacgtggg ccaggatcag cacccgaccg tcccacatct ggacggcgtc gcggcagatc
     8881 gtcgccatca ccgcggtttt ccccccgccc gtggggatca cgacgcacgg gttgtcgtcg
     8941 cggccgcgaa ggtgggtgta gacggcctcc acggcctcac gctggtacgc tcgcagttcc
     9001 atttgcatcg atctccgtga tctcgacgat cgtcttgccg tcggggacaa ccgcgccgcg
     9061 cttgatcacc agctcgtcga tctggctgtc gtcgtggaag gcctcgcctt gctgaagggc
     9121 gtcgagcagg gccttctgca cgttgtccag gtcgcgtcgg cgtgcatcgg gcgggaacac
     9181 cgtcacccgc atgacaagcc gcccgcccag gggttgcgcc cgctgcgccg cgaggagcgc
     9241 taccacctcc ttgcggtagc gccggccctc gcggctgatc agcgtgcgcc agcccactcg
     9301 ccgccagtag tggttgaccg acggcggata cggcagctcg taggtgcggg tcacttctgc
     9361 cacggcgcct tcccgttgtt gccggccggc gccgccggac gcgtcccgtt cccgtccttc
     9421 ttggcgtagc ccttgatcac gttggtgatc tcgtcagtat ccgaacgctt cttatgggcg
     9481 acggtgatga tcagcgggag gtcgtgcagc tcgacgctgt cacggggcgc catgacgccc
     9541 accgcgcggc agatggccga cagctccgcc cgggcgtact tgaccgtctc ggccttcggg
     9601 ttgtcgaggt tcaggcgggt ccacaccagc cggcccttga actcgccctc gagaatctgg
     9661 aacgtcagct ccagatagtt gccggcaccc gacttcgtcg gcttcatctg cgatccgctg
     9721 atgatcgcca ggtacttgcc ggcgggaatc ggatcgaatc cgaccgacgg gtccacttct
     9781 cgcgcatcaa aaccattcag gtttgccatt gcttacgctc cttgctctgc aggttgttcc
     9841 gacatagcca acgggttctc gccgcgaacg aacgcggcgt agatgcgata gtccaggggg
     9901 atctcgtccg gcaggctcag ccggttcttg gcgacatggg cagggcgctc ggtcgtgcgg
     9961 atgattcgct cgcccgtgcc gatgcccttg tgcctgtcct ggccgaactg ctcgccgacc
    10021 ttgcgggtgt gaacgcgata cgtggcgaac agcacctcgt cgcaccactc ctgcacgagg
    10081 gccgaggcca gcttgtgcag gcggggcaca tagcggtcat aggcgtcggt ctcggggttc
    10141 tcgaatcgct cgatcttggc gtgggcgatc agaatggcct gcatgccccg atcggtccgc
    10201 agggcgttga ggccttcgag cacctcgcgc cactgcgtca gcgcgaacgt gtagcccttc
    10261 tggtagccga tgtcctcgat gctctcgaca ccccgggccc ggcagacctc ggcccagatc
    10321 aggcgttcga gccagtcgag gctgtcgagc acgaccgtgc cgaattcgtg cggcgcggtg
    10381 tagagctcgg ccagggcgct cagcacgtcg ccgtacttcg acgccagcgg aaaccgctcg
    10441 cagtcgatgc cgccgagccc gtcctccgtc tggatgaaga cggggcgatc ggccatcgag
    10501 ccgaacgtgg atttgccgat gccctgtgtg ccgtacacca gcacgcgccg gggcatctcg
    10561 gccttgcctc gttggatctg attcataagg ctcatggttc ctccttgtct gttcgtgacc
    10621 gtgaatccgg actcggggcc ggtacaggtc gtcccgactg cggtgggctt ccggcgaaag
    10681 gcctccgcag ccacaccatc cggccccggg tcggggctca cagccggtcg atcgttcgca
    10741 acgcctcgta gcgcgtcggc cagatgccgg ttgcccggca ccgggacagc tgttgaaggt
    10801 cgctttcgtt ctgctcttgg gcggcatcga gcacggcggg gctgatgcgc cacacgccgc
    10861 agcgataggg ttcgcgcttc tcgacggcga tcaggtggac gggcaggatg cggccgctga
    10921 cccgcgcgac cagcgcccgg tagaacgcca actggtgcat gtagccgaac gccgatgccg
    10981 agagctcgaa gctgtcgatg gtgtcggcgg tcttgaggtc cacgatcccc tgatcgcgat
    11041 cggcgctgat ccaatcgatc cgcgcctggc acgcgtggcc ctcgtactcg cagcgaacca
    11101 caccctcggc cgagccgtcg gccagcagct cacgggcaag ttcatgcccc ttgaccgccg
    11161 ccgccatctg ttccacgagg gcggcctggt cgtcgctgag tacggccttg ctctgctgcg
    11221 ccgcccactc ggcaaaggcc ttcgtgttcg acccgaacgg ctggccggtc ttcggattga
    11281 tcgggcctcc gacggcgaac tcgcgggcgt agcgttcgcg cccttcgagg atcagcgtgt
    11341 gggccgcacg cccgatcaga tagaacgtgc tgtcgtgatc ggggatcagg ccgagctgct
    11401 tcttgcggta cagcagcggg cagcgtcgga agtcggccag ggcgtgggcg ctcaggtagt
    11461 ccttggcctt cgcgtggtat acctctgcgg gctcgcggat caggaaacga aggtcgatgc
    11521 cgttcttggg ggtcatgatc tcctcggggt agatgtccct ctggcttgtc ccttggccga
    11581 tccgcacgag cggaaggcat ttgttggttc tcacaggccg gcctcctcga gttcgcgctc
    11641 ggcttccgcg atctcgcggc gacgctgttc ctcaaacgcg gatggtgacc gcaggcgccg
    11701 tcgtcgcggc cccgccgcgg cggcgtgcga ggcggcctcg cacaagtcga agtagcgggc
    11761 gtccgcgtcc gccagtgccc ggccgaactc ctcgagccac cgggcggtgg tgaagatctt
    11821 gccgccgatc cgcatgtgct ggaggcgaac gcgatcgccg ttccgcgacc gtacgccgcg
    11881 ccggcaccag cgccagatgc aattcgccga cggtcggccc ggcgcgatat tggccgcctc
    11941 cgtcagggtg atgtgtgcgt ctgtgatcgt ttgcccgtgc atcgaaacct ccacggcctt
    12001 gccggccatg catcctctgc acgggttctt acaacacgat ttcgcgcggt aactcggtaa
    12061 ctccagttac cgcgctacaa gcggctggcg agatgcgtaa cagcccatgt gacaagcggt
    12121 tacgaattcg cagaaaaatg tggaacgagg ccgggctgcc gagtttggta actcgacagc
    12181 ccggcgcaaa gcttcgcgct ggcgtgacga cgggcgtcag ccgatcttcc gcatgatctc
    12241 aaccaccttg gcgtggtcgc gttcggcgta gacggcgtcg gtgatctgcg cactggcgtg
    12301 gcccaaggcc agttgggcgg cctccaggcc gaactcgcgt cggagcagcg tcgccgcgtt
    12361 atgacggagt tggtggggat gccagcggtg cgcccgttgc cactccaaaa gctgctcctt
    12421 gagctcgggc ttcttcttga gtcgctcacg ccaggccttc ttggtctcct tcttctcgcg
    12481 gccgaagggc aacggcggcg ggtatgcccg gtcacacgcg tagcgaatcg cctgggcata
    12541 cgtgttcgtt gtgtagcggt cgccaggcga gcaggctggc tcatcgcggc gatttgtgcc
    12601 gggacgattg ccgcaagaaa gcggcgtcgt tcgcgcggcg tgcagcgcct cacgacgttc
    12661 ggcctccgcg tccgccgggc tgaacaggaa tgcggtggtc gggcggttgc ccaggaatgg
    12721 tcggatgacc gcttgggcgc gggggccgaa gtagatgatg cgatcgtgct cgcgatggac
    12781 gttcttgtgt ctttccgggc ggtaggtcca gatgcccgcc ttctcgtcga tctccaggtc
    12841 gcaggctcgc agttcgagca actccccggg gcgtgccccg gtaaggagct ggagatcgac
    12901 cagggccctg accgggcggc tgagcagcgg cagcgtagca tcaagcagcg gttgcgatac
    12961 cggccccacc ttacggcctt cgcgggcggt ggtacggccg cgtttgagcg gctcgagcgt
    13021 gcagagcgcc tgatagaccg acgcgggcac catctcgcgg gccacggccc acttgaacat
    13081 gtggcgaatg cgctggacct gggagttgat gaacttgcgt gcccaggctc gacgcggtgg
    13141 aacggccccc tcgtcgccgc gaatcatctc ctcgcgaagg agacgcagct tcttggggcc
    13201 gaactcggcc gcgagtgtct ggccgtagta ctggcgcatc aatcgcagtg cgcagatcaa
    13261 agcctgcgtg tgcttgggcc ggtagtactc ctcggcccat cgccagtaat cgcggaccag
    13321 ttccgtgatc gtcacgctcg tggtggtcgc ggagcaggct cccgcagagt cattggcagg
    13381 caatcggcgg ccagcggcct cccattgggc gatgatgcgg tgatacgcct cacggctctc
    13441 aggcgtgcca tgctcgccga gccagtagtc gcggcgtcgc cgcgttgcgg catccgtgag
    13501 agtgaccagt gcctggctgt agcctttgcg cttacggtag gaaggaacct tcgtcggcat
    13561 gtatcggcct ccgaaacgct tgcgcggtgt aaaaccgcgc ggttttgcgt ctcaagccgc
    13621 actgccgacc gtcggcaggt cctcgtaacg ttcgagggct ggcgtattta cgttcacagc
    13681 gggcgatggg attcgaaccc acgacgtcca gcttgggaag ctggcattct accactgaat
    13741 tacgcccgca ggactgtttc ctgaacggta aagggctcac gcgccgaagt caagaccccc
    13801 gtgatagtat ggcaacgttc ctgcgcagcg gggtctggaa cctcccggcc agatgactgc
    13861 ggcttgcggc gcgtgtttca agaccgcagt gcgtttgggt tgcagcccat gacgaggcgg
    13921 tctgcctcgg cgctgggggt ttgggacgag agtatcggtt tggggagcga ggggcgtagg
    13981 agggggttgg acgccgtaac tgaaatacgt gtccagagcg agcatctgct atgcgggaag
    14041 ggcgtgatgg ttgggttagt ctcttccgtc gggactggtg cgcattttgc gtgcatcttg
    14101 ctggctgcaa ggcgtaaccc gcctgcaaac aagggcttgt ggtcgtaaac ggactgccct
    14161 tcttggttgc ggccgaaggc cgccttgggt tggggatcgg aagaacgata atcggcgacg
    14221 agggcgtcgg gctagcgaat gcttgagaaa gatgcgtcca ggacgggtgg cagtcgggcc
    14281 ggaagaggcc aatgaccgtg ggtggtcagg cggcgttgag gtcaattcca ttgtctttgc
    14341 cagcatcctc acccggcgtt gcgagattct gcacggtcat tcagcattct tttctggcgt
    14401 tgggcagccg attgccggtg aggtgggccg ttggcggttt ttcaaggggg cgtgagccgt
    14461 gggggctgtt cgtgtccggc gcgttgtggt cactgtcggg cggggaacct tctcacaccg
    14521 gttgcggcga atggttccat cgtcctataa gcgataccac ggcagtactg tgcggcgtgg
    14581 aatgagggtt tggtaccggg tatctccggg agcctgaaaa acgcgcgccg ggatggccct
    14641 ccggggtgct tttttctcgg gtctgaagcg ggggcggtaa gcagatatta cgcagcatgt
    14701 cacggaagtc gataaatgat aggtcgttcc gtctgaccgc tcggaccggt ggcggcagcg
    14761 ttggctgccc tctggggtcg tggacaaggg gccgatggtc tccgctggtg gcgggcggag
    14821 gaggtgcggg tggccagtta tccgcagacc taccagattg cccggcaagg cgcggtgaga
    14881 cctgtaacgg tcgggaacga cgaggatgtg atcgcgctgc gcacagagct ggagttgatg
    14941 tccagcttcg cccagggagc gacgccggag ctggtggaag ccctggagga ccggctggct
    15001 gcggtgaagt cgggcgcggc ggaggaaggc gggcgaactg cggaagacat caaggatatg
    15061 gtcgcgattc tgcgcgatgc catgcatccg tcgaccgggg aagccattgc caagcttgac
    15121 gccgactttc cgacagccag gggagaaatc gacatggccg cagtggagaa ggaaagcaac
    15181 gactttcacg aggacgtcgg caacgaagtt acggcgtcgg gcaagccttc tgcggggagg
    15241 gtgggccaag cattcaagca agcaatgctg aacaaggccg tggccgctcg ccgggccgcg
    15301 ccagagacca gcggtgtttc cgagacgccg gctagcgaga atgcaagcga gacgggagcg
    15361 gcctctggtc agcagccggc ttctgcggaa ccggccagcg gtcaacaggt accggccgag
    15421 tcatcggcgg gaacgacgga gtccgatccg gtggaggcgg cgctacgaga agcgatggga
    15481 atagccgagg gcgcacttgc cggtgagacc agcgatatgt caggcgtcgg cgacgagttc
    15541 gacgaggaag gcttgtcggt tgaggatctc gaagccgagg gggtcacgga aggtgaaaag
    15601 gcggatgagg tggagggcat gaccgagggc gactcactca ctgggaaagc cggagttgcc
    15661 gaggggactg ccggtggcga ggcgacagtc gaccctctcg aacagatcat ggctgagcag
    15721 gccggcttgc cggatagcga ggtctcgctc gaccagcttg cggacatgtt gcttgccgga
    15781 caggcgaatc cttcaccggc gggccaagag tcggtgacgg aaggcagcgg aatcgacgcg
    15841 actgacgcgg ttgatgcctc gcgatcgatt ccggagaacg gcggccgtgc tcaagaggtg
    15901 acggacgcaa gggatcagac ggaggtggac aaagcagatg cgagtcagaa tacggcttgc
    15961 gtgccgaaag atgaggcggg tcagccggac ttggcggcgg aagaggtcgc tcaggccatt
    16021 gaggccagcc gtaccgagat cgatgccatt gcttccgcgt tcgaagaggc taccgcggac
    16081 ctgggcgcgc tggcggatca agtatgcgat agtgcgattc cgccgggtga ttcggtgctc
    16141 ccggaagcgg agttgatcgg ggcggccgag ataccggaag ccgctcaagg gatggagttg
    16201 tccgcggatg aggtcgccga gatcagcagc gatctgaacg ctgccggcat ggaggacgag
    16261 ccggcatttc aaacatccaa tctgcccagc ctgaacatgc gcgaagagtt gcgtacggtt
    16321 cgcgagacga ttcagacagg tctcgagagg ttgattcagt tgttggatca catggaccgg
    16381 acgcacatcg aagccgagaa caggttggct cgggccaaga tgtaccagca ggccgcccgg
    16441 cgggctcagg aagcgagtga gaagctggta caggctcgaa cggaggcggc ggatgccagg
    16501 gccgcctacg aaagcgccca gcggcggctt gaggaagcgg agtcagcgtg ggaagaagcc
    16561 cgccgtgcgg cggatgatgc ggccttgtga ggggggcgtg cggacggcaa ggtcacccac
    16621 accccggccc acaagactcc gcgctgagcg gagtatcatc atcgcacctc cacccatttg
    16681 gtcgcgatgg gcgtcgccgg gattgttgca aacccatggc cagacatctg tgcggcttgg
    16741 gcgtaccttt tcggctcggg gtgcgctttg gctgcgaccc gcaaaaaggc ggtccgtgtc
    16801 aggacgcgct ggaggcagac agcgtttccg acggtctact cgccgatgcg ccactccagg
    16861 attccgccgg agaagccggg ctgcaattcg accttgatgc ggatttcgat tgcgtcgacc
    16921 ggttcgaatg acaccttgtt gaaggtatcc ttttccacgc cgtagggacc ggtgttcttg
    16981 acggaggtcc acggtccgcc gctcttgggc cggtactcga tcgtccatga cttagggacg
    17041 cggcaggagc cggtgccgcc gtcgtcaaac cagtatacct cgcagtatcc tatcgtttga
    17101 ggctcataca gcgcgcgttc gacccattcg ttcgtgccgc ggtgaggcca ccaagtaaaa
    17161 cgacgaatgg attgatcact cgagctcttg gggatgacgc catcggccac ggcctggagg
    17221 ctgtcggccg ggtttgaatg cgaggcggtc aacgccggct taggtggggg tttcttccag
    17281 gtcttggcgt cgggcccgtc gccgattcgc gggaaagctg cgatgcgcag gcgagcgcac
    17341 cccatcggga tcagcgtgag ttcttcgacc ggttggtcgc tgcggacggg gctttcagga
    17401 acctcgccca ccagcccgtt cttctcctgt tgccactcgg gaatacgttt gcccttggcc
    17461 ttgagcatta ccggagcggc ctcagggcgg aacggttgct gaccgacgcg gacgccggtg
    17521 cgtttgaatt caaaggacgt ggccggatct ccgggattga cgatcaggcc gtagttccac
    17581 ggcgtggcgg ggaagacctc gtaggccgcc cacggcttgc cgttgtcgta ttcctgccac
    17641 ttttcgccga tcttgagcga gtaagtgagc ggtccgcggc tgaccgaaac gctgttgcgg
    17701 ttggccgtcc agacgtgggt gctgatttcc atgggcaact cgatgcgcac cttgtcgcca
    17761 tcggaccact tgcgtgtata gacaacccag cgcgaagaca cgggctcctg ggcttggtcg
    17821 ttgatgcgaa tgtgggcaag cttgcaccag cccgggatac gcagcgagag cgggaactca
    17881 acggactggg ccatcgagaa gtgcaggtct atgacgtcat cgaacgggta gtcggtcttt
    17941 tcgtcgatgg tgactttcgt tccgtcgccg acccttgcgg tgaccttgct gggggcatac
    18001 atggcggcgg ccaggccgtt gccgggtgtg gccatccaga ggtgctcgac gaaatagggc
    18061 cagccgaaag ccacgttgtg ttggcagcag cggtactgct cgtatggcgt gtatgacatc
    18121 atgtcgccgc gattctcgat catgggggtc ttgtcggccc ggtcgcattg aatctggttc
    18181 gggcaggtca ggtagtgcag gcctttgagg tccggtgtga gcgacggcgg aaaggagttg
    18241 aacgccacct cctcgcagcg atcggcccag aggccatcgc cgctgatctt cagcaggatt
    18301 tcatggctgt gcatcatctc gacgaacgcg caggtttcgg ttccctgtcg cgggtcgtgg
    18361 aagccctcac ggcaattctc gtcggctccg tacattccgc cggggacctg gccgaacttg
    18421 gcgcgcactt cgtcgtaatt gcgggcggca gcggcgaagt gtttcggatc gtgactctgg
    18481 acgtagtatt gtgccggttc gcggaagcac tccgcgatgt tcacgccatg ccaactggcg
    18541 atgccgccgg tccagtcggc ggtgcgttcg tggttgacct tggccaggtc gagcagccac
    18601 ggctcgccgg tctgattgta gagccagtat atgctatcga gatgatcgcc ggcccgaatc
    18661 cactgccacc agccggcgcc gtactgttta ccccacgtgt agagcttgtc tacggggatc
    18721 ttctgcacgt agcggaagta gttggtcatc acggaaatga ccttggggtc tccggtggcc
    18781 tcgtagtagg agcgcatggc gtagaggatg cacatgtgcg gccagaggtc gttgttcaca
    18841 aagttgtcct tgggaccgaa ggtgccatcg gggcgttggg agtcgaggat ggcccggatc
    18901 cacttagtgg cctcggccat gatccgcggg tctttgagca gcaggccaag gtcgacgtaa
    18961 cctttgagcc aatagggcat ctcttcccag ggccacttgc cggtgccgtc gggtgtcacc
    19021 cacgcgcttt cagcaaagtt gcaccactgg ctgatttcgg cgaggtgccc aaccatgcct
    19081 tcggcctgga attgtagctg gctgcggagc cagcctttgg gctcgaccgc gccgacgggc
    19141 agtttgatca gaggaccggg cagcaagggt gcccggttgc cgatgtagaa ggagttggag
    19201 ccgtcggcgg cgaggcgatc gtgcatgacg accttggtag tggtgcatgc cggaattgcc
    19261 agagtgatca gcaggaagag agcaaatcgg cttgggtggt gcagtgtccg tttgagcggc
    19321 gtgcgagagt ccttgttcaa gctgtgcatc agtattctcc atttactgcg cggtgcgcgc
    19381 ggtccagaag gaggccggca tccgaaccgg tcggcaagtg ggtgacacct cgcggctccg
    19441 gcggatccgg cttggccaag gctctgccgt ggcaatcgcg ccgccggcgg caactcttcc
    19501 gggtgtcacg gactgccggc cgatgacggt cgagcattat agcgacccac gcgagtggag
    19561 ggtagaatcg gctctgcggt gcgtacatcg ggtgacgcag ggtcgacgag gggagacggt
    19621 catccaccgt gaggcgacat atgaatccgg atttgggcac ggtcacgttt ttgagttttg
    19681 cgatgctgtt ttgtgccggc gcggctcccg tgcagaagtc cagggagaaa ctggggcggg
    19741 ggtctcttcc cgcggagtcg tccgcgcaag aatcgcaggt gcagcccgcg agtaagacgg
    19801 aggcgccggt cgtcaaggcg acgtcgggcc agattcagat cgtattgccc cccggtgagc
    19861 atgccggcaa ggtggtcgtg tacgagcttc ggccgaacga gaaggacgat gcctggcggg
    19921 gcaaaaagcc gatcgagacg gccggtatgg ctgcccggcg gatggtcaag ctcaacgtgg
    19981 accgtttcga gcggacgccg ggcggtcggc gggatcggct atattccaag ttcgtggtgg
    20041 tgccggtgaa cgggcaggag ccgattatgc cggcccgttt cgtggaggat ttcacttcgc
    20101 tgcagcgccg caggctgccc tttcctcgaa cacgttccaa gaagggtttg caggtgcaga
    20161 tggtcgatga cgcgattgcg cttggtgtgc agcatgcggc tcttaacgtc gatctggcca
    20221 gactgtttgc ctggacgccc atgccggacg cgattcccca cgtggtcgac ggcgagcggt
    20281 ttctcctgaa tccgtcgttg gtcgtcgatc tggaccgacg ggtgaccatg ttgtcgcagg
    20341 cggggatgac ggtcacactg attctcctga attacgcgaa caccgcggaa gctggggcgg
    20401 cagaggtgtt acggcatccg aactacaacg acggctgccc gaaccagatc agcgcgttca
    20461 atacggcgac ggccgacggt ctgaggcatt accgcgcggc cgtggagttt ctgatcgacc
    20521 gctacaccca gcctgatggc cggttcggtc aggcgctggg ctacatcatc ggcaacgagg
    20581 tcaactcgca ctggcactgg tgcaacatgg gcgaggtgag catggaggag ttcacggagg
    20641 attatcttcg ggcgatgcgg attgcggcga cggcagccga gaagatctgt cccaccgcac
    20701 gcgtctacat gtcgctcgag catcactgga acgcgaggtt cgattcgcag accaaatctt
    20761 ttcccggtcg tgcctttctg gagcttttcg ccaggcgggc gcgggagcag ggcgactttg
    20821 cctggcacct ggcttatcac ccgtacccgg acaacctgac ggagccggcg ttctggcgag
    20881 accgggccgc gacgaccgat gtgaacacga cgcccaagat cacgctcaag aacattgagt
    20941 tgctgcccga gtacctgcga cggccggaat tgctttacaa aggcgagcct cggcgcctga
    21001 tcctgtccga gcagggtttc cacaccaagg ccgggccgga cggtgagata ttgcaagccg
    21061 cagcgttctg cgcggcattt gtcaaggttg ctcacattga cggcatcgac gcgttcattc
    21121 tgcatcggca cgtggattac cgggatgaag gggggctcaa actggggttg tggacccgca
    21181 acgaagcgtc tccgggtccc ggcgaccccg gacgacccaa gcggattcac gaggtttttc
    21241 ggatggcgga cacgccgggc tgggcggcgg cttttgagtt tgccaagccg atcatcggaa
    21301 tccgggattg ggcggagctg ttgccgccat cggcgggcca gccccttccg tccaggaagt
    21361 gaggagatcc gatagcgaag ggcgattgcg cggagatgat cgcccgacgg acgcgatccg
    21421 tgcgcggcga catactcggg cgagggttgt gtgctacctt ggttccactg ctccgcgatc
    21481 gctgctgcat tcgacgcgga atcgcttgcc gcggaaggag atgttcattt tgagatgtcg
    21541 ccagttagcc ggcagggcgg gggtcgcggt aggggattcg cccctgaggt tcaggccggc
    21601 aaagcccatt gcgacggtca gccagacgcc gcccatgttg gcggtgtgga ggccttcggc
    21661 cgtgttgcct gccagatcgt tgaggtccag ataggcggct cgtcgaaagt agctgtaagc
    21721 ttcttcgatg tggtcgaggc tcgcggcggc ccacgcatgg gcgctgacgc tcagcgatga
    21781 gtcgtggctg gtccgaggtt catagtacct gtagttggcc tcgcgtgatt cgcggctgaa
    21841 ctggtcctcg agcagggtct ggaggagcac cacctcggcc tgcttgagca cctgactttc
    21901 gatagccatt cctgcaccga tgatctccgt gatcgaggta ctccgcgtga tgcgtagaat
    21961 atccggcgag acctccggcc ggtcaaagaa accggcgtgc tggttgatga ggccggtttg
    22021 ttcgtcgcag agcagaacga tgttcttcgc aatgtctcgc caggtggcga cttcgtcgga
    22081 gtccaagccc agttgggcgg caaggtctct tcgtttgctt ttcgggaagg cggcgttgtc
    22141 ccacaggtcg gcggcggcct ggaggttcca gcgggccatg tagttggtaa aggcgtcgtt
    22201 gtcgacgtgg atgtgaaact catccggtcc cattgcatcg cggatctcga atcgaccggc
    22261 ggcgttgcgc gtgacgcggc tggcccagaa gcgggcggtc tcgaacatga cctccgcccc
    22321 gtgctcccag aggaaatcct cgtctccgct gacgcgaacg tactggtcga tggcgaacga
    22381 tacgtcagcg gagatatggt cctggatgtc gccgcaccag atacggtgtt tctcgccggt
    22441 ggtcgggtcg gtccagcttg cggggcaggt ctcatcgccg gtgtcggcgc tttcccacgc
    22501 atactgggcg ccgcggtagt tgttggatcg tgccttgcgg cgggcgccgt ccaatgttgc
    22561 acgacgatag gtcagcaggg tcttggcgcc ttgcggattg gtcagggcga agaacggcag
    22621 catgtagatc tcggtgtccc agaagacgtg gccaccgtaa tgcgtgccgc ccaggccttt
    22681 gggggcgatg ccggcgatct cgccgggcgg cgggcagagg gtggcgagct ggaagaggtt
    22741 gaaacggatg gcctgttgat cctcgaccgg tccgtcgatc tcgacgtcca tttggtccca
    22801 gagttccgcg caggcgacga tgtgatcttc gagttgttcg ttccatccgc tcttggcggc
    22861 ggacagggca gcgtttcggg cggccttggc gggtgattcg gcatccggcg atccggccat
    22921 gaaggcgaca aagcggtcaa agcagtagac ggcgtcttct gagacgtcga ggatatacga
    22981 ctggccggcc gtacagggat cggaggaggc ttcacccggt atggggttct gctggtctac
    23041 ggcgacgtaa gcaccggcca cggccagcgg gcgccgactt tggctggtcc gggtttccag
    23101 ggtcgccaac ccgtgggccg aggacgtgag cttgggcggg tcccagtgcg agaccttgcc
    23161 gaaagcggcg ggctcgctat gccggatgcc cgttttgagt tcgaccggtc cggaccagtc
    23221 aaccgggacg attcggatgc gcaccgcgag gatattgcga ttggccaggc tgacaaatcg
    23281 ttgaaaagtc aggcgcgttg tgtggccggc cgcgtcttgc caagtgactt cgcggcaaag
    23341 caaccctcgt ttcatgtcca gccatcgcga aaagctgcgg atatgcccgc gacgcaagtc
    23401 aaaaggctgg tcgcacaaca cgatgtgcgt ggtcaaagca tctggaaggg ggggcagttc
    23461 cggcagaccg tcaggcaggg tgtcatagag accggccatg agggtcagat ttcgagatcg
    23521 cgtgccgatc atcggctcct cgaagctgcc gcggataccc agcagcccat tgctctggct
    23581 gaacagcgat tcccagacgg gtatgcgcag ccaatccatt tcaccctgga gaatggtgta
    23641 acgatcgtcg cgaggcggaa aatcgccgat ggtcatgagt agggctccga ggagaaaaag
    23701 cctccagccc atcagatcct caggtaatca ggacaagcga aaggggcgat gagcttgcct
    23761 gacgtgcccg gatcgtgcaa ggggctgata ggctaacggt cttaagcgaa caatcctttc
    23821 acgtatgcca gattcttctt tgtgtcagcc acgatatcct tgttcgggct gccggtttcc
    23881 aggacgatcc agccgtcgta cttgatctcg cgcagcactc tcactgtggc cggccagtcc
    23941 accttgccgc tgcctttttc gagcatcgga tagtccttga aatggatctg gcagatttta
    24001 ttgcggccca gcaggcgcat ttcttcgagc ggatcatgct tgacgtgggc gttgttgtaa
    24061 atgtcgtaat agacctgcag cgcgggcgac ttcacttggt cgaggatgcc gatcagggtc
    24121 ctggccgaga ggtagctctc gaagcccaga atcactccgg ccttttcggc ccgcggggcc
    24181 agctcgacca gcgcctcggt gacccgccgg aggtcttcct tggcatcttc ctttaactcg
    24241 ccctttccga agaaagccag caacatcgac ttggcaccca gtctggcggt ggcttcgatc
    24301 ccatcggcga cccagagcgc ggctttcggt tcggacacca gcgggacgtc gttgagcacc
    24361 cccatggccg tcgaaggaat cgacaagccc gttctgtggg ctgcttccat gtaggctttc
    24421 tgcacctcgg ccctgcgaag ctggagacca tcttttggat agccgatgct gacctcgacg
    24481 ccgtcgaggc cgatctgctt ggcaacgtcg aatacgccgg ggtcatcgcg gccgatggac
    24541 cagtcgcaca tgccgaactt catgcccgac ttgcgggttt catcggccat ggcttgcagc
    24601 cgcgtgagag ccaagccggc cgctgcggtt cccgcgcctg ccagccattg tcgtcgtgaa
    24661 agcttcatgg gcccattcct ccgcccataa gaccaagatg ggaactgcac gccttacgct
    24721 cccaaaggtg gcaggcaaag cctgccggtc aatcctccgg caacggctta tccttcgtct
    24781 cgggtgccca gatcaaggcg atgattccga ggaagtagaa gccgcacagg atgaccgcgc
    24841 cccagcggaa gacctcgtcg acgccggctt tcttcaaggc gagttgaagg cctccgccca
    24901 acagtatgat gacggcggtc agcacgcgag cgacgttgta gcagaaagcc gttcccgtgg
    24961 agc
//
LOCUS       DAOTPW010000057        24777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2096_length_24777_cov_5.446485, whole genome shotgun sequence.
ACCESSION   DAOTPW010000057 DAOTPW010000000
VERSION     DAOTPW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 24777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24777
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_24777_cov_5.446485"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..2511
                     /gene="pilM"
                     /locus_tag="P5316_07135"
     CDS             364..2511
                     /gene="pilM"
                     /locus_tag="P5316_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRR84768.1"
                     /translation="MAANRPVWGIDLGQCALKAICLRPTEDKEKVEVLDHVYVEHSRI
                     LSHPDADRPALIAEAMKKLTEQHDLSKEILVVSVPGQHTLARFTKLPPLENKRKIPEL
                     VHFEAQQQIPFDMDEVVWDYQVFEDPKSGEIEVGIFAMRRDLLREQLQYLTGINLEPA
                     VVQSSPLALYNALRYDGICTDEPVCILDVGTQNTDLIVVVEGRSLWTRNIPIGGNAFT
                     EVLLKTFKLSFGKAERLKREAQKHKYARQIFQAMRPVFADLVAEIQRSIGFFTSSRRG
                     VRLSKIIAMGNAFKLPGMVKFIQQNLGMDVFRPASFNKLAVGNVSNAAVLTENLLSYG
                     VAYGLALQGLGLSQITSNLLPTEIAKQVVWRRKTPWFYGAAAALALSAAIVWGRNLAD
                     AGAVSAGRGSEPAPSFQVQYDPNDKDRENPLPDPAAEAVLKGSFTAQSPMGRAREIAA
                     VADHFNEVLTAIAGKNDARATQAEDMVKLEVNEAVWPNILYMIHSALPQPDPVMKQAL
                     EQGPEAVKKLVQSDPKYERSKRREIYILSLTSQYSPDVLTEFKALGEDKSGMRPPTSG
                     YMPDPSATDAAAEPVQANLEGFLINFVIRSPNQQQFGYINDEFLVKLREGVVPEVAYV
                     AKVRVPQRAQLKDGGILGSVGGMGSGVSRAPTAGGQPTVEALLDPVTGESMREDWEYA
                     GTMAVVLGKKPDQPPASPVPDGTGGQPEPEAGM"
     gene            2613..4013
                     /locus_tag="P5316_07140"
     CDS             2613..4013
                     /locus_tag="P5316_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84769.1"
                     /translation="MQWIKSHLVIVIVASVSLLSLTGLVLGYVMSSVGADLETDAREL
                     TKLQGIKRVNPKVIDAARKQQQEDAARLADYFRKYQEAKQYTPLDDQVFADNITDSQR
                     QAAIFRFQDKYQPKSLLQLLKAKDQPTAEEFTEYEQEIKAKQDKIEKELGVAGGGRPS
                     EVLTAPTAPRAGALGQTGTSADQDASRRLRMAYAVDRAKQVYCYASEQSLDDRTAEVK
                     TVGGTPPIEDLWYAQVALWIQEDIFRALGGLNEASAKELPEEARWVAYLPVKRLIKFT
                     MGNYVPRGAAGDAEGGGSALRGGPMGGGGGGTGAELLTAGPTAVFTKRVSSETVDVVR
                     FHLDMVVDARRLLDVLDAISKAGFYTPLNVDLREVVPAPEEYYIYGTAPVLQMTVVYE
                     GSFMRSSYDKFMPTAVKNAIAEGRAGGLGKAGAGLGGTGIRPPAAFPGHRGTPRGGRG
                     EYREDFESERPRSRRN"
     gene            4076..6061
                     /locus_tag="P5316_07145"
     CDS             4076..6061
                     /locus_tag="P5316_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84770.1"
                     /translation="MAKQNVTYVERHVEKIAAGVAGAVLLFVSVTSLLMSPHAVRIAG
                     DSVKPAEFYGKVADQAEAARRQLEGASEVPMELAEKSKRLHEMLDQTAKAPPNELPVA
                     AVPLSPALPEVAGEFISAPVGAKVRLVQILPPTRPILSTGKAFARLQTPRTDQPGVSV
                     NTATDDLMVTTDLHWVVGAAVFDRKAQRDRFAKANYLSDRQEVIVAEVRVERREQLPN
                     GQWSDPVVVQPYMNRRILARAVLNTLAEADGVILPEDEQRYAADLRQMLYSKETQADI
                     LRPEFQSYLADPTPDSDDTIQWQIPRSLTAADGTQVDMTAPEFGVQFFEEEGRPGSKT
                     GPGAATDGGAQASPAVRLQKALKEAQDAINQKRWLDAQRILDDIIASTDLTPAQKKPA
                     EELRKRHLRDFEEAERLAARAGQAQEREVLGPDNDPLWFTDISVQPGKAYQYRVCLMV
                     FNQYVGMSSRLENPADAGKLLLQGDWSEWSDAVTVPAAKHFFLAEVPRDGERISIELW
                     EWKGGDWVKAKAELLPGDPVVAARNPRDKIDYGAILVSADPSRPYARRSGNPKSEAFK
                     YDIQETGAAVLVKAEGQIEEHFVAESADRRAEVLRAMAEDRRRRETFKPQGGAIAPRV
                     RQPPVRPPRERFSDEEPRRSESRREREDRGDRTRGRR"
     gene            6450..9758
                     /locus_tag="P5316_07150"
     CDS             6450..9758
                     /locus_tag="P5316_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84771.1"
                     /translation="MKTATNTMRRMAMLALGLCATLPVLAATPSELLDKAVELFREGK
                     YQQAQEVLLEIDREQLPADRRDQRDELAEVITTAVNQSVKATQNLEDAVAAQAGNDLN
                     TAEKLFQAVTENKYATEAQKRKAAEGLELIKKQRDLAGKIEAAQPPSTRPTGPAASRP
                     ADAYGKAREERARIEAAVKIKAGEDATAKGQYDLAEQHFQEALKLVPNHPAAIHGLQL
                     VKQHRSVEGKSSLIEETANRKLLRWKRTVSLYQEGVREVRGLINERRYDEARRRVADT
                     GQLLDAGRQDASPPSDYEALKRELDGLARRIEVEEKDALEAKAIADRKATLETDRRRQ
                     WRVEQEKMERIGELFNQAEQLKRQKEYEKAAELLREAIAIDPTYERAKWQLEAMEDAG
                     LIDDQRRNREHFLNQMQDALLEADRAKTPVVTGKNDKIIAFPDEEQWRLISSRDPYGV
                     DGVGGETEADRIAAKALSTPLPPDVQFAQGTTFRQAIDRLKEVGRIPINVNWSTLSMV
                     GIDPDGAEVGDLNVGGVKLETALEMVLDVAGGVVGNIELGFDVLEGIILVSTVEELNR
                     KTEVRVYDIRDLLLRIRSLSIGQGGMGGMGMMGGMGMMGMGMMGGMMGGMGGYGMGGM
                     GGYGMGGMGGYGMGGMGGYGMGGMGGYGMGGYGGGGGYGTGGTGGEDEEEDDREEQID
                     NLKELIQKLVEPGTWEEEGGGNIGSLDVWNDRLIVRHTRRAHRQLRELFRQLREARDL
                     QVAVEARFITLTNNFLEEIGVDLDVILNQGNAGLDQAIVPSTGGTGGYQQAVNPITGM
                     GLLQTRQMTQYGFLPASVSAGVPLTQQGGFVQPFGAAGLVPQGSPENWWDRHTTAVPL
                     LNSTMGLASPRPTGIPGGYGPQAYPAFSLFGSFLDNIQVDFLLKATQLDSRSSIVDAP
                     RLTVYSGRRAEIQVQTSSYFVSAPGQLPVGGTGINGQAAIGRPPSISMAPSGRTFSVK
                     PTVSGDRKYVTMELIPTVYASELQDVVDATGPMKLPNISTTSIATTVTVPDRGWLLLG
                     GLKQAGETEVEAGVPILSKIPILKRAYTNRSRVKDERTLLLLVKPTIIIQGEIEEEAF
                     PNLVTVNQGGIGGG"
     gene            9822..11093
                     /locus_tag="P5316_07155"
     CDS             9822..11093
                     /locus_tag="P5316_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRR84772.1"
                     /translation="MTKTVPIRLPLFQRFDCHRCGYCCRNLVVNVDADERRRIISAGW
                     AARLAGTRLFESYRLSGRRYYCLAHRPEGGCIFLRQDGLCRLQAESGREIKPLACRLY
                     PFAPSPGADVVRVDLRFDCPSVAANKGRPLSVHAAEIASLATEIGARPMSHAPSWRGQ
                     RSLTAREFTSIAAAFEGLLRRESLSIRLRLLAGCRLLDLVYGLEIAKVRDDRFDELMT
                     LLADAVFEEVRKASPGLPSAVAPARAGKLFRQWVFLHSLTEGPQDRALGLAGRWIRSW
                     RRYRESRSFSAGGGPIPACRPEWPSVRFEDLGRVGPAADEALEPICRSMRVKLETHAF
                     SGPAYFGYETLAGLTALWLMPAVVGWFARLEALAKGLPTLTAEAVIGGLRQVHHTFGI
                     SPVFGRISERFRLRALARPGIPAAILAEYGP"
     gene            11315..12352
                     /locus_tag="P5316_07160"
     CDS             11315..12352
                     /locus_tag="P5316_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR84773.1"
                     /translation="MARPVTLFTGQWADLKFDVICKKAKKLGYDGIELPCWGDHFEVS
                     KALEDRSYCKRKWEVLQKNGLACYAISNHLVGQAVCDLIDERHKSILPPHVWGDGKPE
                     GVRTRAAAEMMDTARAARKFFDAAPGTVKKNLKRVVVNGFTGSSIWYLLYSFPPVLPG
                     QIDAGYKDFAKRFIPILDVFDREDVYFGLEVHPTEIAFDIASAARAIEAVKGHKRFGF
                     NYDPSHFGYQGVDYVRFLREFGDRIFHVHMKDVYWSSTPTRAGVFGGHLDFGHPDRSW
                     EFRSLGHGCINFEEIIRTLNQIGYDGPLSVEWEDAGMDREHGAAEACAFVRKVDFPSS
                     QIVFDAQFAKK"
     gene            13291..14418
                     /locus_tag="P5316_07165"
     CDS             13291..14418
                     /locus_tag="P5316_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR84774.1"
                     /translation="MAKEILITGASGFLGRALAKGLLLESDDTLHVLVRSDKAECTLR
                     RHFGDLVSSRVHFVRGDITESGCGVEQEVRTRLNGKIDELWHLAASTTFDDTRADEIR
                     RANVGGTENILSLARHFDRLDRFYYMSTAYVCGKRTDVIDEDNIDASEGFKNVYENTK
                     WQCERIVRASGLPFTIIRPSILVGDSRNGDSMGEARMMYGYLLALYHAALHAVGGSAE
                     YSRYWHRAATGRWINVNARLYGSGDVTKNVVTVDDVIRVCMAIRASGSLAENQTFNVV
                     NSRNLPIAYIVDSMQRAMRLAGYYYDASLPPTGLVTDNKVERAAYRSTRPFFPYAKYT
                     EPSWVCNHRPISDVSRVTMTEELFDFLMRRFVEDTLVSNAR"
     gene            14408..15391
                     /locus_tag="P5316_07170"
     CDS             14408..15391
                     /locus_tag="P5316_07170"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HRR84775.1"
                     /translation="MLGDTVQRFSFQQRLDYLRQFGTHCMAYSTLQPGMRYHDMPAVG
                     YIAYRQYCGTRFVLGDPVCAFADIPRIVGEVLREHPQTCFVQIREHTATMLRDEFGLR
                     SLHMGVETLLPLARWNLTGKRKQVVRTARNQAMERGITVEEDGSSPREELARISEEWL
                     GTRRVKSRQMSFLVRPFDMQLNGDTRMFVARRDGRLTGFAVFDPIYSQGRVIGYTPDI
                     TRSSQDFRQGVYYCLVTAAAERFKEEGAEILNLGLSPLAVNNGTPRSESRLVTLGLEL
                     LFERGNRFYNFKGVCFTKSRFCGTEIPVFFSHPNRYPFREILSVLSLSNVL"
     gene            15521..17149
                     /locus_tag="P5316_07175"
     CDS             15521..17149
                     /locus_tag="P5316_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HRR84776.1"
                     /translation="MATMVNSPAPVFPRTEEIPENLRIGYYEAGARYLIDGRVHTWTG
                     PCEEVLSPVCVAGADGAVQYRLGHYPLMAEAESLQALDAACKAYAKGCGDWPTMSVAD
                     RIRHVEAFMPRMQAVRDEVVKLLMWEIGKTLPDATKEFDRTLEYVADTIDALKDLDRI
                     SSRFAVEQGFVAQVRRSPLGVVLCMGPYNYPLNETFTTLIPALIMGNTVIFKPPKYGV
                     LLHAPLLQAFADSFPPGVVNTVYGDGPVVVGPLMTSGRVDVLAFIGSARVASILKHQH
                     PRPNRLRCVLGLEAKNAAVVLPDADLDLAVKECLLGATSYNGQRCTAIKIIFVQRRLA
                     ETFCGKFCDALGKLKVGMPWEEGVTITPLPEQGKPEWLAELVSDAVTAGARVINEAGG
                     TIDHTMYYPAVVYPAKPDMRICQVEQFGPVTPIVPYDDEQEVIDWLTESPYGQQVALF
                     GSDPEQLSEIIDSAVNQVCRVNLNSQCQRGPDKFPFTGRKDSAEGTLSVSDALRVFSI
                     RSLVAAKATDYNEQIISKIVIGRHSRFLSTDFIF"
     gene            17375..18319
                     /locus_tag="P5316_07180"
     CDS             17375..18319
                     /locus_tag="P5316_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRR84777.1"
                     /translation="MMSYQDIHVVTGAFGYSGRYIARRLLDRDCAVRTLTNSTARRHD
                     FGDRIEVHPFHFGEPEKMRRSLAGASVLYNTYWVRFNHKGFSHAEAVRNTISLFEAAK
                     AAGVQRIVHVSITNPSEDSPLEYFRGKAALERYLRDLGVSYAILRPAVLFGKEDILIN
                     NIAWLLRRFPFFGVFGDGQYRIQPIYVDDLARLAVEQGLSRENVVINAIGVETFTYRQ
                     LVATIGEIIGKPRRIISVSPTCGYVLSRVIGRLVGDVVLTREEIEGLMQGLLYVNAPP
                     AGQTRLTDWVRRHADTLGLRYTSELARRRDRTSRYASN"
     gene            complement(18595..21132)
                     /locus_tag="P5316_07185"
     CDS             complement(18595..21132)
                     /locus_tag="P5316_07185"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR84778.1"
                     /translation="MMNLIRQTPARRTVCASLLIAACTSGVCAATSPDIDSAVAVEKR
                     KREFLGRLADDWAAAGHPIQWKHEEWKFDEIEPNWSGALAAMHLKRNREEVSKADAFF
                     AAMPLDEKIDPDMRVCEAIHAYYLFRDDPSLSPAARKRLLDIMHALPAPRRINPSIWE
                     FGATENHAFMGHTWNLMTAQLDRDHNAVAEMSRHIALFIIEHIRKGWLEYNSPCYVEK
                     EIGCLVMVAEWAEDPRLRQIAELGLDVLFAEHAALNLEGMLCGPACRVYQPAHDGILP
                     AEFNHNSRRDAQCSGSYPCMYMLFDQGRPHYYGVLGAPLLATSRYVPPKAVTALATAG
                     PERGCYEFKARRPGRYHNLLRAKPGQSSPPPEVFSSRVYAWVTPDFILGSFQEVDGLF
                     GASRALPLSSVLRISGSTRRAVYADLVPAEREASATSTVDCVQYKNVVLGRGSVGQAY
                     LATKEFDEVAEQQGWIFARAGGTFLAYRVIGAAWKWAGADDPSVFGDFVKFENPQAPF
                     VLEVAGTSDYKGDFSRFRADVLDNTIKQEENTVSYESCSQGNAGPSAERFTLTLRYGQ
                     LPLLDGQPLDLEGYGTFESPYLNSAWDSGIVNLRYGSERLMVNVTRLERVIRTEETIT
                     PLSLPYQTDCEKPDAAWVPFLNYWRLKPQQWYWSNDGGKPGGCLRHDAARGVDNAERG
                     AHDAMILLRGGETWADYSFEADARSDKGTLGLWFRADMEDEGGGNGRRVQGYYLVIDP
                     AHKKCRLWRARKDGLQSADAKGEPGPAEVNHFSNPLLIAEQQTPDPIAAGSWIHLHVE
                     VKGSKIACSVNGQTVITTEDGTYPSGSVGLFAYKGMDVRFDNIRVSP"
     gene            complement(21543..23576)
                     /locus_tag="P5316_07190"
     CDS             complement(21543..23576)
                     /locus_tag="P5316_07190"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR84779.1"
                     /translation="MKPTSMKALLALAMTTVWFSSAIAAIDTESLLNEMTDLRRLATL
                     PEPPYVTKQFSSWDRASKSHSDFQGYFANGDCGQYLRIEDKDGRKEHVMMDTPGPGAI
                     VRIWSANPKGTLRIYLDKADKPAIETPMTKLLGGEVPGFPKPIAGERSRGWNLYFPIA
                     YAKHCKVTSDEGGFYYHVNYRTWPAGTEVKTFTMEDTQRLADKIKDVADRLSNPRQGG
                     GPPDERTKSPFEVSLAPDKEAVLWEMEGPKAICGFLVRLDAGDLDAAARQIVLYMSFH
                     GDKKDEKTGQPVLEKTVESPIGDFFGTAPGLTPYDSLPLGITKDKPQDLWCHWWMPFN
                     KKAVIRIKNLGEQEVRVSGAVAVMPWEWNDKSLLFHAKWRIEKNLPSRPMTDWGHLAC
                     SGHGRFVGGSLHIINPVKDWWGEGDEKIYVDGETFPSHFGTGTEDYYGYAWCCNERFV
                     HAYHNQPRCDGPGNFGNTSVNRFHIIDDIPFTKSFKFDIENWHWNPVCDTTRAAISYW
                     YAKSGCWDDWFFGPITRDDVKIEPMPVLPINKVPGAIEGETMEVVEKTGATSTQELGG
                     WSSENHLWWTQGKPGDKLTLAFQAKEAGSKHVIVRLTKAVDYGIVQLYVNGQKAGDPI
                     DLFQADGVGRTDELDLGTFDLKEGRNLLTAEIVGANSKAKKDYMFGLDYLLLK"
     gene            complement(24076..24462)
                     /locus_tag="P5316_07195"
     CDS             complement(24076..24462)
                     /locus_tag="P5316_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84780.1"
                     /translation="MTAEIVGQAQRRLDLGRPRHEVAEESDLQYETPGARGKLMGLDR
                     IPEVCCLGKRLSQLSEDEAPQQWAAVLSQRWMEQNPDDWHSRCDTPLMNRNRQYSPIL
                     PKERRGVVRHGASFHEPIAKPWRLHE"
BASE COUNT         4756 a         7128 c         7954 g         4939 t
ORIGIN      
        1 gggaagttcc ctgacggtct tcctcagggg gcggcttttc accgtcgcgg ttgacaaaga
       61 gccgcttgaa ctatcacgct ttcgcgaggg cgctcttccg tgtccagctt gacatagagc
      121 cgccacctct gaatactcta gtagacgtga accctagcga gggccgtctg cctgcgacgc
      181 gttttcaaga cgaggtcacg ccaaccgagc tgatagctcc ctgaggattg cgatcgtccg
      241 ggtgatgggc gacgcagtcc gctccgatgg ccgagttgat ggttttcgac aagtggaaaa
      301 caggatccca ggcgagccgc ggcggtcccg atcgggcgac cggcggacta caaggagaag
      361 ccgatggcag ccaacagacc ggtgtggggg attgacctgg gccagtgtgc cctgaaggcc
      421 atctgccttc gtccgaccga ggacaaggag aaggtcgaag tcctcgatca cgtctatgtc
      481 gagcattcgc gcattctgtc gcatccggat gccgaccggc ccgctctcat cgccgaggcg
      541 atgaagaaac tcaccgaaca acacgacctg agcaaggaga ttcttgttgt gtctgttccc
      601 ggacagcaca cccttgcccg gttcaccaaa ttgccgccgc tcgagaacaa gcggaagatt
      661 cccgagttgg tgcactttga ggcccagcag cagatcccct tcgacatgga cgaggttgtc
      721 tgggactacc aggtgttcga ggatcccaag tcgggcgaga tcgaagtggg cattttcgcg
      781 atgcggcgag atctgctccg agagcagctc cagtatttga ccggcatcaa cctcgaaccg
      841 gcggtggtcc agtcgtcgcc cctcgcgttg tacaacgctt tgcggtacga cggcatctgc
      901 acggatgagc ccgtctgcat cctcgacgtc ggcacgcaga acaccgacct gatcgtggtg
      961 gttgagggta ggagtctgtg gacgcgcaac attcccatag ggggcaatgc ttttacggag
     1021 gtcctgctca agaccttcaa attgtcattt ggtaaggccg agcgactgaa acgcgaggcc
     1081 cagaagcaca agtatgcccg gcagatcttc caggcgatga ggccggtttt tgctgatctg
     1141 gtcgcagaga ttcagcgatc gatcggtttc ttcacctcct cccggcgcgg cgtgcggttg
     1201 agcaagatca ttgcgatggg taacgccttc aaactgcccg gcatggtgaa gttcatacag
     1261 cagaaccttg gcatggacgt gttcaggcct gcctccttca acaagctggc ggtcggcaat
     1321 gtttccaatg ccgccgtttt gactgagaac ctgctcagtt acggcgtggc ctacggcctg
     1381 gctctgcaag ggctgggtct ttctcagatc accagtaatc tgttgcccac ggaaatcgcc
     1441 aagcaggtcg tctggcggcg caagacgcct tggttctacg gcgcggcggc ggcgttggcg
     1501 ttgtcggcgg ccatcgtctg gggccggaat ctcgccgatg ccggcgcggt ttcggccgga
     1561 cgcggcagcg agccggcgcc cagctttcaa gtgcagtacg acccgaatga caaggatcgc
     1621 gagaacccgc tgcccgaccc ggccgccgaa gccgttttga aaggcagttt cacggctcaa
     1681 tcgcccatgg gcagggccag agagatcgca gcggtcgccg atcacttcaa cgaggtcctc
     1741 accgcgattg ccggcaagaa tgatgcgagg gccacgcagg cggaggacat ggtcaaactc
     1801 gaagtcaatg aggcggtctg gccgaatatt ctctacatga ttcacagcgc tttgccccag
     1861 ccggatccgg tgatgaagca ggcgctggaa cagggtcccg aggcggtcaa gaagctggta
     1921 cagagcgatc cgaagtacga gcgatcgaag aggagggaga tttacattct cagtcttact
     1981 tcgcaatact cgcccgatgt gctgacggag ttcaaggccc tcggggagga taaatcgggg
     2041 atgaggccgc ctacatccgg ttacatgccg gacccaagcg cgaccgatgc ggcggccgag
     2101 ccggtacagg caaacctcga ggggttcctc atcaatttcg tcattcgatc ccccaaccag
     2161 cagcagttcg ggtacatcaa cgatgagttt ctcgtgaagt tgagagaagg ggttgtcccg
     2221 gaggttgcct acgttgcgaa ggtgcgcgtg ccccagcgcg ctcagctcaa agatggcggt
     2281 attctgggtt cggtcggcgg catgggctca ggcgtcagca gagcacctac ggccgggggc
     2341 caaccgaccg ttgaagcgct cctcgatccg gtcacgggcg aatcgatgag ggaggactgg
     2401 gaatacgccg gtaccatggc agtggttctc ggaaagaaac ccgaccagcc gccggcgtcg
     2461 cccgtgccgg atggcaccgg cggtcagccg gagcccgagg cggggatgta gccggcggtt
     2521 ttggggaccc taatgggctt tgccggtccg accagcgcga tcggacaagg tgagccggtc
     2581 ggtgcctcgg cagagacagt ggagactgga tcatgcagtg gatcaaatct catctggtga
     2641 tcgtgatcgt ggcctcagtc agcctgttgt cgttgaccgg gctggtactc ggttacgtga
     2701 tgagctcggt gggggcggat ctcgaaaccg acgctcggga gctgaccaag ctacagggta
     2761 tcaaacgcgt gaatccaaag gtgattgacg cggctcggaa acaacagcag gaggacgcgg
     2821 ctcggctcgc ggactacttc cgcaagtacc aggaagccaa gcagtacacg ccgctggatg
     2881 accaggtttt tgctgacaat atcacggact cccagcgaca ggccgcgatt ttccgcttcc
     2941 aggacaaata ccagccgaaa tccctgcttc aactgttgaa ggccaaggac cagcccacgg
     3001 cggaggagtt cacggaatac gagcaggaga tcaaggcgaa gcaggacaag attgaaaagg
     3061 aattgggcgt cgcagggggg ggccggccga gcgaggtgct gaccgcgccg acagctccca
     3121 gagcgggtgc cctcggccag actgggacga gcgcggatca ggatgcgagc cgcagactgc
     3181 ggatggccta tgcggttgac agggcgaagc aggtgtattg ctacgcaagc gagcagagcc
     3241 ttgatgatcg gaccgcggag gtcaagacgg tcggcggtac gccgccgatc gaagatctgt
     3301 ggtatgccca ggttgccctg tggatacagg aggacatctt ccgggccctc ggcggactca
     3361 acgaggcttc tgccaaggag ttaccggagg aagcaaggtg ggtcgcttat cttccggtca
     3421 aacgcctgat caagttcaca atgggcaatt acgtcccccg aggagcagcc ggcgatgctg
     3481 agggtggcgg ctcagctctt cgcggaggac cgatgggggg tggcggaggc ggaaccggcg
     3541 cggagttgct cacggcggga cccactgccg tatttacgaa gcgcgtttcg agcgagaccg
     3601 tcgatgtcgt gcgatttcac ttggacatgg tggttgacgc acgacgtctg cttgatgtgc
     3661 tggatgcgat ttccaaggcg ggtttctaca cgcctctcaa cgtcgatctc cgcgaggtgg
     3721 tgcccgcacc ggaagagtac tacatctatg gaacggcccc cgtcctgcag atgacggttg
     3781 tttatgaggg gagcttcatg cgaagctcgt atgacaagtt catgccgacg gcggtcaaga
     3841 atgcgattgc cgagggtcgt gccggaggac tcggcaaggc cggagcaggg ctcggcggga
     3901 caggaataag accgccggcc gctttcccgg gtcatcgtgg aactcctcgc ggaggtcgag
     3961 gcgaataccg cgaagacttt gaaagcgagc gtccccgcag tcggcgcaat tgatctcggg
     4021 cgcgcgacac accgcgcgcg tgtgacatac cgggccggtt caggaggtcc gacgaatggc
     4081 aaaacagaat gttacttacg tcgaacggca cgtcgagaag attgccgcgg gagtggccgg
     4141 ggcggtgctt ctgttcgtct cggtaaccag tctgctgatg agccctcacg ccgttcgaat
     4201 cgccggtgat tcagtcaagc cggcggagtt ctatggcaag gtggccgatc aggccgaggc
     4261 ggccagaagg cagcttgagg gggcttccga ggttcccatg gagctcgccg agaaatccaa
     4321 gcgactccac gagatgttgg atcaaacggc caaggccccg cccaacgaat tgcccgtagc
     4381 cgcagtgccc ctcagcccgg cgctgcccga agtggccggc gagttcattt cggcgccggt
     4441 cggcgccaaa gtgcgcctgg tgcagatcct tccacccacc cggccgatcc tctcgaccgg
     4501 caaggcgttc gccaggctcc aaacgccccg gaccgaccag cccggcgttt ctgtgaacac
     4561 cgctacggat gacctgatgg tcacgaccga cctgcattgg gtggttgggg cggcggtttt
     4621 cgaccgcaag gcccagcgag acaggtttgc caaggcgaac tatctttcgg atcgccagga
     4681 ggtcatcgtt gccgaggtcc gtgtggagcg ccgcgagcag ttgcctaacg gtcaatggag
     4741 tgatcccgtc gtggttcaac cttacatgaa ccggcgcatc ctggcccgcg ccgtgctcaa
     4801 cactctcgca gaggctgatg gcgtgattct gcccgaggat gaacaacggt acgccgcaga
     4861 cctccgtcaa atgctctata gcaaggaaac gcaggccgac attctccggc cggaattcca
     4921 gtcttatctg gcggacccga ctccggacag cgatgacacc atccagtggc agattccacg
     4981 aagtctcact gctgctgatg gcacccaggt ggacatgacc gcgcccgaat tcggggttca
     5041 gttctttgaa gaagagggac gccccggaag caagacgggc ccgggcgcgg cgacggacgg
     5101 cggcgcacag gcatcgccgg cggtgaggct gcaaaaggcg ctcaaggagg ctcaggatgc
     5161 catcaatcag aagagatggc tcgatgctca aaggatcctc gacgacatta tcgccagtac
     5221 cgacttaacg cccgcgcaga agaaaccggc agaagaactg cggaaacgtc atctgaggga
     5281 tttcgaagaa gcggagagac tggctgcccg cgctgggcag gctcaggaga gggaggtgct
     5341 tggtccggat aacgatcctt tgtggttcac cgacatcagc gttcaaccgg ggaaagcata
     5401 tcagtatcgg gtctgcctca tggtgttcaa ccaatatgtc ggcatgagca gccgtttgga
     5461 gaatcccgct gatgccggca agctcttgct ccagggagat tggtccgagt ggtctgacgc
     5521 ggttaccgtc ccggcagcca agcatttctt cctcgccgag gtcccccggg acggcgaacg
     5581 tatcagcatc gaactgtggg agtggaaagg cggcgattgg gtgaaagcga aagcggagtt
     5641 gctcccgggg gatcccgtcg tggccgccag gaaccccaga gataagatcg actacggcgc
     5701 catcctggtc tcggccgatc cttcgcggcc gtatgctcgc cgtagcggca acccgaaatc
     5761 ggaagcattc aagtacgaca tccaggagac gggcgcggcg gtactagtga aggcagaagg
     5821 gcaaatcgag gagcacttcg tggcggaatc cgcagaccgc agggccgagg tcctccgcgc
     5881 tatggccgag gatcgcagac gccgcgagac cttcaaaccc cagggtgggg caatcgcgcc
     5941 ccgcgtgcgt cagcctcccg ttcgacctcc acgagagcgg ttttcggatg aggagccgcg
     6001 ccgatcagag tcccggcgcg agagagaaga cagaggtgat cggacgcgcg ggaggcggta
     6061 gtattgacct aagctcttta tccggcgaat cttaccgcag gtcagatact tcgttcagtt
     6121 atatttcgtt gactttttga cctgcggttt gagggctggc gaacttgagg cgactgcggt
     6181 aagctgagcg gcctgagcgg cgcgcgaggt gcgtccgcca gacggtcggc ggaagagtgg
     6241 agacgcgccg gttccggaca aggcctgtgt ttgatccgcg gccgcgagca gcgcgtgaga
     6301 cccgtgcggg gcgggacgaa ggctgagaaa caacaagtga tgcggcgttg gttttgccgc
     6361 agcctgcgtt gaccaccata cttcaggtgg tcgctgacac ctacctgtga tgtgtgccct
     6421 cccgagtggg atgcatagga gacccaaacg tgaaaacggc aaccaacacg atgcggcgga
     6481 tggcgatgtt ggcgctaggt ctttgtgcga ccttgcccgt tctggctgcc actcccagtg
     6541 aactgctcga caaagccgtc gagttgttcc gtgaaggcaa gtatcaacag gctcaagagg
     6601 tgctgttgga aatcgatcgc gagcagctcc ccgcggatcg tcgggatcaa cgagacgaac
     6661 tcgccgaagt gatcacgacc gccgtcaacc aatcggtcaa ggccacacag aacctggagg
     6721 acgcggttgc agcgcaggcc gggaacgact taaacaccgc cgagaagctc ttccaggcgg
     6781 tcactgagaa caagtatgcg accgaggccc agaagcgcaa ggccgccgaa ggcctggaac
     6841 tgatcaaaaa gcagcgggac ctggccggga agatcgaggc tgcccagccg ccaagcactc
     6901 gtccgacggg tcctgcggcg tccaggcctg ccgatgccta cggaaaggcc cgcgaggaac
     6961 gggcccgtat tgaggctgcg gtgaaaatca aggccggcga ggacgcgacc gccaagggcc
     7021 aatacgacct ggcggaacaa catttccaag aggccctcaa gcttgttccg aaccatcccg
     7081 ccgcgatcca cggcctgcaa ctggtcaagc agcaccgcag tgttgaaggc aagtccagtt
     7141 tgatcgagga gacggccaat cgcaagttgc ttcggtggaa gcgaacggta agtctctatc
     7201 aggagggcgt ccgggaggtt cgcggtttga tcaatgagcg acggtacgat gaggctcgtc
     7261 gtcgcgtcgc ggacaccggg cagttgctcg atgccggcag gcaggatgcc agcccgccat
     7321 cagactacga ggccctcaaa cgcgagttgg acgggcttgc ccggcgcatt gaggtcgagg
     7381 aaaaggacgc cctggaggcc aaggctatcg cggaccggaa ggccacgctc gaaaccgatc
     7441 gccggcgaca gtggcgagtc gagcaggaga agatggagcg tatcggagag ctcttcaacc
     7501 aggccgagca actcaagcga cagaaagaat atgagaaggc ggcggagttg ctcagagaag
     7561 ccattgctat tgatccgacc tacgagcggg ccaagtggca actggaagcg atggaagacg
     7621 ccggactgat cgacgaccag aggcggaatc gcgagcactt ccttaaccag atgcaggatg
     7681 ctctgctgga ggccgaccgg gccaagactc cggttgtgac cggcaagaac gacaagatca
     7741 ttgcctttcc cgatgaagag caatggcgat tgatcagcag ccgcgatcca tacggcgtgg
     7801 atggcgtggg tggtgagact gaagccgatc ggattgcggc caaggccctc agcactccgt
     7861 tgccgcccga cgtgcagttt gcccagggta cgaccttcag gcaggcgatc gaccggctta
     7921 aggaggttgg aaggatcccc atcaatgtta actggagcac gctctcgatg gtgggtatcg
     7981 atcccgacgg ggccgaggta ggtgatttga atgtcggggg cgtgaagctc gaaaccgctc
     8041 tggaaatggt tctggatgtg gcgggcggag tggtcgggaa cattgaactg ggctttgatg
     8101 ttctcgaagg cattattctg gtgtccaccg tggaggaact gaaccgcaag accgaggtcc
     8161 gtgtttacga catccgcgat ttgcttctgc gcatccgcag cctctccatc ggtcagggag
     8221 gcatgggcgg catgggcatg atgggcggta tgggcatgat gggcatgggc atgatgggcg
     8281 gcatgatggg cggcatgggc ggctatggca tgggcggcat gggcggctat ggcatgggcg
     8341 gcatgggcgg ttacggcatg ggtggcatgg gcggctatgg catgggcggc atgggcggct
     8401 atggcatggg cggctatggc ggcggcggcg gctatggcac ggggggtacg ggcggcgagg
     8461 acgaggaaga ggatgatcgc gaggaacaaa tagacaacct caaagagttg attcagaagc
     8521 tcgttgagcc gggtacgtgg gaggaggaag gcgggggcaa catcggttcg ctcgacgtgt
     8581 ggaatgatcg cctgattgtc cggcataccc gccgtgccca ccggcaactt cgcgagttgt
     8641 tcaggcagtt acgcgaggcc agggatcttc aggtggcggt ggaggcccgg ttcattaccc
     8701 tgaccaacaa cttcctcgaa gagatcggcg tcgacctgga cgtcattttg aaccagggca
     8761 acgcgggcct tgaccaagct atcgttccct cgaccggcgg caccggtggt taccaacagg
     8821 cggtcaatcc gattaccggt atgggccttc tgcagaccag gcagatgacg cagtatgggt
     8881 tccttccggc atccgtcagt gcgggtgtgc ccctgacgca acagggcggt ttcgttcaac
     8941 cgtttggagc tgccggtttg gttcctcaag gaagccctga gaactggtgg gacaggcata
     9001 caaccgccgt tccgttgttg aacagtacca tggggctggc ctcgccgagg ccgacgggca
     9061 tccccggcgg ctacgggccg caggcctatc ctgcgttttc tctgtttggc agcttcctgg
     9121 acaacattca ggttgacttc ctcctgaaag caacccaatt ggattcgcgg agttcaatag
     9181 tggacgctcc gcgattgacc gtgtacagcg gccggagggc cgagatccaa gtgcagacct
     9241 cctcctactt tgtgtcagca cctggtcaac tgccggtcgg cgggacgggc atcaacggac
     9301 aagcggcaat cggccgtccg ccttcgatct ccatggcacc atctggacgg accttctcgg
     9361 ttaagccgac cgtctcgggg gatcgtaaat atgtgaccat ggagctgatt ccgaccgtct
     9421 atgccagtga actgcaagac gtggtcgacg cgacgggacc catgaaactc ccgaatatca
     9481 gcaccacctc tattgcgacg acggtgaccg tccccgatcg cggctggctg ctcttgggcg
     9541 gcctgaagca ggccggtgag accgaagttg aagcgggtgt cccgatcctc agcaagatcc
     9601 cgatcctcaa gcgggcctac accaacaggt cacgcgtgaa agacgaacgg acgcttctgt
     9661 tgctcgtgaa gccaaccatc attattcagg gcgagatcga ggaagaggcg ttcccgaatc
     9721 tcgtcaccgt caaccagggc ggcattggtg gaggctgagc gcagcccgag accggttgtc
     9781 cgagcgggtc ctgcgcacgg tgtgcgtgcc gtggcaagcc catgaccaag acggtgccta
     9841 tccgtttacc gttgttccag cggtttgact gccatcggtg cggttactgc tgccgcaacc
     9901 tggttgtcaa cgttgacgcc gatgagcgac ggcggatcat ttcggccggc tgggcggcca
     9961 gactcgccgg gacaaggctt ttcgagagct atcggctctc cggtcgccga tattactgtc
    10021 ttgcccatcg gccggagggc ggctgcatct ttcttcgtca agacggtctc tgccggttgc
    10081 aggccgagag cggtcgggaa atcaagccgc tggcctgtcg cctgtaccct tttgctccaa
    10141 gtcccggcgc ggatgtcgta cgagtcgatc ttcgcttcga ctgcccctcg gttgcggcaa
    10201 acaagggacg ccctctgagc gtacacgccg ccgagatcgc gtcgctggca accgagatcg
    10261 gggctcgccc catgtcgcat gccccgagtt ggcggggtca gcgaagcttg acggcgcgag
    10321 aattcacctc cattgcggcg gcgtttgagg gtcttctgcg acgggaaagc ctgtcgattc
    10381 ggttgcgact gttggccgga tgccggcttc tcgatctggt ttacggtctg gagatcgcga
    10441 aggtccgcga cgaccggttc gatgagctga tgacactgct ggccgatgcg gtctttgaag
    10501 aggttcggaa ggcctccccc ggtctgccgt ccgctgtcgc gccggccagg gccggcaagc
    10561 tgtttcgcca atgggtgttt ctccattcgc tgaccgaggg gccgcaggat cgggccctcg
    10621 gcctggccgg gaggtggata cgttcgtggc gacgatacag ggaatcccgc agtttttcgg
    10681 ccggcggcgg ccccattccc gcctgccggc ccgagtggcc gtcagtccgt tttgaggatc
    10741 tgggccgggt cggcccggcc gccgacgagg ccctggaacc catctgtcgg tcgatgcgcg
    10801 tgaaactgga gacccacgcg ttttccgggc cggcctactt cggatacgag accttggccg
    10861 gtctaaccgc cctttggctg atgcctgccg ttgtcggctg gtttgcccgg cttgaggcct
    10921 tggccaaagg attgcctacc ctgaccgccg aggcggtgat cggggggctt cgccaggtac
    10981 accatacgtt cggtatttcg ccggtcttcg gccgaatcag cgagcgattc cgcctccggg
    11041 cgttggctcg accgggtatc ccggctgcga tcctggccga gtacggtccc tgacgacggg
    11101 gcgacagaca gcttgtgtcg acctgcccga ttgccaaccc tgtccgaccc gtggtaacat
    11161 ttgcggcctg aataggctgg cggcaggcgg tttcggcccg aacaagaagt atgaggttct
    11221 tacgggcgat ttgtgccgct gggttgaagt gcggattcgc aggggcgccg gcttgggcgc
    11281 cggttggtct tcgttgatga aaggatggcc tgaaatggca cgacccgtca cgctgttcac
    11341 cggtcaatgg gcggatttga agttcgatgt catctgcaag aaggctaaga aactcggcta
    11401 cgatgggatc gaactgcctt gctggggaga tcacttcgag gtcagcaagg ctctcgagga
    11461 ccgcagctac tgtaaacgga aatgggaggt gctgcaaaag aacggtctgg cctgctacgc
    11521 gatcagcaac catctggttg gccaagcggt ctgcgacctc atcgacgagc gccacaaatc
    11581 gatccttccg ccgcacgttt ggggcgatgg gaagcccgaa ggcgtccgca cccgggccgc
    11641 cgccgagatg atggacacgg cccgcgccgc ccgcaagttc ttcgatgccg ccccgggcac
    11701 cgttaagaag aacctgaagc gcgtggtcgt taacggtttc accggcagca gcatctggta
    11761 cctgctctat tccttccccc cggtcctgcc cggtcagatc gacgcgggct acaaggattt
    11821 cgccaagcga ttcattccca tccttgatgt gttcgatcgg gaggatgtct acttcggact
    11881 ggaagttcat cccaccgaga tcgcgtttga catcgcttcc gccgctcgag cgatcgaggc
    11941 ggttaaaggg cacaagcgat tcggcttcaa ctatgatccc agccacttcg gctatcaggg
    12001 ggtcgactac gttcggttcc tcagggagtt tggcgaccgg attttccacg tgcacatgaa
    12061 agacgtgtat tggtcgagta cgccgacacg agcgggggtg ttcggcgggc acctggactt
    12121 cgggcacccg gaccgttcgt gggaattccg gtcgcttggc catggctgca tcaatttcga
    12181 agagatcatt cgaacgctga accaaatcgg ttatgacggc ccgctgtccg tggaatggga
    12241 ggatgccggc atggaccgcg agcacggtgc tgccgaggcc tgcgcgttcg tccgcaaggt
    12301 cgattttccc tcgtcccaga ttgtcttcga cgcgcagttc gccaagaaat gaggggcatg
    12361 atgcgaaacc cgtctttccc ggcgggacca taggcaacgg caaaagcctg cggacctgcg
    12421 tctgcaggct ttcttcttgt cgtcatcacg tcagaaacgg cagtttggga aagatgtcct
    12481 ccgatagcgc ggggtcggga aggcttcgtc tcggccgatc agcgtccaca tcgaaggcgg
    12541 aagtaacggt tattcgccga ggtgaccaga aggcattcta cccctcgtta tgggggcatc
    12601 accgcccaat cgaaaacagg agaaaccggg ctgttgatag ccgcacgagt atgtgcttgg
    12661 ttttcttttg aatcccgttg tccgtaaaga gttatcgatt gccaaaaggg ctgtcgcgac
    12721 cgctgagcgg gctcacgacg catcgccgcc acctaggata acttcctaag cgtcagcgct
    12781 tccatggatg cccatagcgg tcgcgtgtct cttagcaact cttcagaatt cgattactct
    12841 gatgcgtatc attttctggc aaatatagtg gatggtgatc ggggtgcgcc attatcggac
    12901 cgaacccgtg gtgctgttat tggaggtgac agatgattgg gtcgtggggg cactttggca
    12961 aggtgcgcaa cgggagaaag gtggtaactt gggtgattaa gatgagcccc atgtgagaca
    13021 tacgcaattt ttgcgctttg tcttctttga gaaggcggat gcctgcaact acaaaatcgg
    13081 gtggttgtac cgaagttcgg gcaaaaaact cttgaaaaaa aagcgcgatt gctgctacac
    13141 tgacatcccg cagtgtggca ttgaggagga gtggtacgaa ggacttgaaa cgggaaagtt
    13201 gcggacagct cgcgaaatcg actggttggc ggcttgcggt ttgcggcctg tcaggaccgc
    13261 gggcgtgtcg acagggggag gtcgattgag atggccaaag agatactcat cacgggagcg
    13321 agtggttttc ttggtcgagc actggccaag ggtctgctct tggagagcga cgacacgctt
    13381 cacgtgcttg tcaggtctga caaggctgag tgcaccctga ggcgccattt cggcgatctt
    13441 gtttcgtcgc gcgtgcattt tgttcgaggg gatatcaccg aatcgggttg tggggtcgag
    13501 caggaggtcc ggacacgact caacggcaag atcgacgagc tgtggcatct ggcggcctcc
    13561 acgacgttcg atgacaccag ggcggacgag atacgccggg cgaacgtggg cggtacggaa
    13621 aacatcctgt cgctggcaag gcatttcgac aggctggacc ggttctacta tatgagcacg
    13681 gcctacgtct gcgggaaacg cacggacgtc atcgacgagg acaacattga cgccagtgag
    13741 ggtttcaaga acgtctacga gaacaccaag tggcagtgcg aaaggatcgt acgagcttcg
    13801 gggctgcctt tcacgatcat tcgcccgagt attctggtcg gcgactcacg aaacggcgac
    13861 tcgatgggtg aagcgaggat gatgtacggc tatctgctcg ccctttacca tgcggccctt
    13921 cacgccgtcg gggggagcgc tgaatactcc cggtattggc accgggcggc cacaggccga
    13981 tggatcaacg tgaatgcgcg gctgtacggc tcgggcgacg tgaccaagaa cgtggtcacc
    14041 gtcgatgatg tcatacgggt ttgcatggca atccgggcct cagggagtct ggccgagaat
    14101 cagacgttca atgtggtcaa cagtcgcaac cttccgatcg cgtacatcgt tgattcaatg
    14161 cagcgagcga tgcggctcgc cggttattac tatgatgcct cgttgccgcc gacgggtctg
    14221 gtgacggaca acaaggtcga gcgggccgcg tatcggagta cgcgtccatt ttttccctat
    14281 gccaagtaca cggagcccag ttgggtctgc aatcaccgcc cgatttcgga cgtgtctcgc
    14341 gtgacgatga ctgaggagct gtttgacttt ctgatgcggc ggttcgtgga ggacacgctc
    14401 gtgtccaatg ctcggtgaca ccgtgcagcg tttctcgttt cagcagcggc tcgactatct
    14461 tcggcagttc ggcacacact gcatggccta ctccacgctc caaccgggca tgcggtacca
    14521 cgacatgccg gccgtgggtt atattgccta ccggcagtat tgcgggacgc gttttgtcct
    14581 gggagacccc gtctgcgcgt ttgccgatat acctcggatc gtgggagagg tccttcgcga
    14641 gcaccctcag acctgttttg tccagatccg tgagcacacc gcgacgatgc tccgcgatga
    14701 attcggtttg cgttctctgc acatgggcgt ggagacgctg ttgccgctgg cccgctggaa
    14761 cctgaccggc aagagaaaac aggtggtcag aacggcccgc aaccaggcca tggaacgtgg
    14821 catcaccgtt gaggaggacg gcagcagccc gcgtgaggag ctcgcgcgaa tctccgagga
    14881 atggctgggc accaggcggg tcaagagcag gcaaatgtct tttctcgtgc gaccgtttga
    14941 catgcagctc aacggggaca cccgcatgtt cgttgcccgg cgcgacggac gactgacggg
    15001 ttttgccgtg ttcgacccca tctactcgca gggccgagtc atcgggtaca cgccggatat
    15061 cacgaggtcg agccaggatt tcagacaggg ggtctattac tgcctggtta ccgccgctgc
    15121 cgaacgattc aaggaggagg gcgccgagat tctcaacctt ggtctttcgc cgctggccgt
    15181 caataacggt acccctcgct cggaaagccg gctggtgact ctcggcttgg agctgctctt
    15241 tgaacgaggc aaccgcttct acaatttcaa gggcgtatgt ttcaccaagt cccggttttg
    15301 tggtaccgag attccggtct tcttctcgca ccccaaccgc taccctttcc gcgagattct
    15361 ctcggtgctg agtctgtcca acgtgctgtg ataccgccac tcgggcaagc atggcaggat
    15421 ggctcattct gcgggcgtgt tgtgtcttcg ccgcgcggtg atacactgtt gagccgacga
    15481 cggacgttct tgtcgccttt ccgcttgttg gaggctttcc atggccacaa tggtcaactc
    15541 acccgcgccc gtcttcccgc ggaccgaaga gattcccgaa aacctcagga tcggctatta
    15601 cgaggccggg gcccgttacc tgatcgatgg gcgggtacat acctggacag ggccgtgcga
    15661 ggaggtgttg tcgcccgttt gcgtggcggg tgccgacggg gcggtgcaat acaggctggg
    15721 gcactacccg ctgatggccg aggccgagtc gcttcaggct ctcgatgcgg cctgtaaggc
    15781 ttacgcgaag ggttgcggcg actggccgac gatgtccgtt gcggatcgca tccgtcacgt
    15841 ggaggcgttc atgccgcgca tgcaggcggt tcgcgacgag gtggtcaagc tgctcatgtg
    15901 ggagatcggc aagaccctgc ccgacgcaac caaggagttc gatcgcacgc tcgagtacgt
    15961 cgccgatacc attgacgcac tcaaggatct ggaccgcatc agttcgcggt tcgcggtcga
    16021 gcagggattc gtggcccagg ttcgccgcag cccgcttggt gtggtactgt gcatggggcc
    16081 gtacaactac ccgctcaatg agacgttcac gacgttgatc ccggccctga tcatgggcaa
    16141 cacggtcatc ttcaagccgc cgaaatacgg tgtgcttctg cacgcgccgc tcctgcaggc
    16201 ttttgccgat tccttcccgc ccggcgtggt gaacacggtt tacggagacg gaccggtcgt
    16261 tgtcgggccc ttgatgacca gcggccgggt cgacgtgctg gcgttcatcg gttcggccag
    16321 agtcgcatcc atcctcaagc atcagcaccc gcggcccaac cgcctgcggt gcgttctcgg
    16381 tctggaggcc aagaacgcgg ccgttgtcct gcccgatgcc gatctcgatc tggcggtcaa
    16441 ggagtgcctg ttgggcgcaa cgtcgtacaa cggccagcgg tgcacggcga ttaagatcat
    16501 ttttgttcag cgccgtctgg ccgagacgtt ctgcggcaag ttctgcgacg cgctcggcaa
    16561 actgaaagtc ggcatgccct gggaggaggg ggtcacgatc acgccgctgc cggaacaggg
    16621 taagcccgag tggctggccg aactggtaag cgatgccgtt accgctggtg ctcgggtgat
    16681 caatgaggcc ggcgggacga tcgaccacac gatgtactat ccggcggttg tctaccccgc
    16741 caagccggac atgcgcattt gccaggtcga gcagttcggg ccggtcacgc cgatcgtgcc
    16801 gtacgacgat gaacaggagg tcatcgactg gctgaccgag tcgccttacg gccagcaggt
    16861 ggccttgttc ggctcggatc ccgagcagtt gagcgaaatc atcgattcgg ccgtcaacca
    16921 ggtgtgccgg gtgaacctca acagccagtg tcaacgcggg ccggacaagt tccccttcac
    16981 cggccgcaag gactcggccg agggaacgct gtcggtctcg gatgctctga gggtgttctc
    17041 gattcgcagc ctggttgccg ccaaggccac cgactacaac gagcagatca tctcgaagat
    17101 cgtgatcgga cgtcactcgc gctttctgtc gacggatttc attttctaga ctgccaggct
    17161 cttcggctgt tagactttta gaaccttgcg gccggatatc tgtgcggctt gcgcgcatgt
    17221 ttccggatca cagagccctt ggactgcggc ccgcaaaaag gcggtccgcc ttaggatagg
    17281 tggtaggccg aggcatgacg atgaaggacg gcactatcga attcgttgcc ctcagcttct
    17341 tccgaatagt ctaacggcct aacagtctga cagtatgatg agctatcaag acattcacgt
    17401 cgttaccggc gcgttcggct attccggccg gtacatcgcc aggcggctgc tcgatcggga
    17461 ttgtgccgtc cgcacgctga ccaactcaac cgcccgtcgg cacgatttcg gcgatcgcat
    17521 cgaggttcat cctttccatt tcggcgagcc ggagaagatg aggcgctctc tggcgggagc
    17581 gagcgtgctg tacaatacct actgggtgcg tttcaaccac aagggcttct cccacgcgga
    17641 agcggtgcgg aacacgatct cgctgttcga ggccgccaag gccgccggcg ttcagcggat
    17701 cgtccatgtg agtatcacca atccgtctga ggattcgccg ctggagtact ttcgcggcaa
    17761 ggccgcgttg gagcggtatc tgcgggacct cggcgtttcg tacgcgatcc tgaggccggc
    17821 cgtcctgttc ggcaaggaag acatcctgat caacaacatc gcctggctgc tcagaagatt
    17881 cccgtttttt ggggtgttcg gcgacgggca gtatcgcatc cagccgatct acgtcgacga
    17941 tctggcccgg ttggccgtcg agcagggcct cagtcgcgag aatgtggtca tcaacgccat
    18001 cggagtggaa acgttcactt accggcagct cgtcgcgacg atcggcgaga tcatcggcaa
    18061 acctcgacgg atcatttcag tctcgccgac gtgcgggtac gtcttgagtc gggtgatcgg
    18121 gaggctggtc ggggatgtcg tgctgactcg ggaggagatt gaggggctta tgcaaggcct
    18181 gttgtatgtg aatgcgccgc ctgccggcca gacgcgcctg actgattggg tccgccgtca
    18241 cgccgatacg ctcggactgc gttacacgag tgagttggcc aggcgtcgcg accgtaccag
    18301 ccgctatgcc tcgaattgag agacgagtcg tcactcttca gttctcagtc gtcagtcgtg
    18361 gggcgtaaga atgtgtgtga agacggcagg gcatggtcgg agccctgcga agtctgacgg
    18421 acgaagcagg gcggagacct gccggtgttg gctgcagaat atggcaggtc tcggtcgcca
    18481 aggcgacctc gacactgccc taccggaccg ctccgtcggt tttcgcacgc agtccgaggc
    18541 gattgaagcc gtgaccacga accttgttgc cgtcttctga agaaccgtga actatcaagg
    18601 tgagactcga atgttatcga atcgcacgtc catacccttg taggcaaaca agcccaccga
    18661 gccggatggg tatgtgccgt cttcggtggt gatgaccgtc tgtccgttga ccgagcaggc
    18721 gatcttgctc cccttcacct cgacgtgcag atggatccac gacccggcag cgatcggatc
    18781 gggggtttgc tgctctgcga tgagaagggg attggagaaa tggttgacct cagccggtcc
    18841 gggttcgcct tttgcgtcag cggattgcag gccgtccttg cgagcccgcc acagcctgca
    18901 cttcttgtgt gcggggtcga tcaccaggta gtagccttgg actcgtcggc cgttgccgcc
    18961 gccctcatcc tccatgtcgg cccggaacca gagacccaac gttcctttgt cggatcgggc
    19021 gtcggcctcg aagctgtagt ctgcccatgt ttccccacca cgcagcagaa tcatcgcgtc
    19081 atgtgctcct cgctcggcgt tgtcgactcc gcgagccgca tcgtggcgaa gacatccgcc
    19141 gggcttgccg ccgtcgttgc tccaatacca ctgttgcggt ttcagccgcc agtagttcag
    19201 gaacggtacc cacgccgcgt ccggcttctc gcaatcagtc tggtaaggca atgacagcgg
    19261 cgtaatcgtt tcttcggtgc ggatcacccg ttcgagtcgt gtgacgttga ccatcagccg
    19321 ctcgctgccg tatcgaagat tgacgatacc cgagtcccat gccgagttga gataaggtga
    19381 ttcaaacgtg ccgtatcctt cgagatccaa cggctggcca tcgagaagag gcagttggcc
    19441 gtacctcagt gtcagggtga agcgttccgc tgacggcccg gcattgccct gcgaacagct
    19501 ttcgtagctc accgtgtttt cttcctgctt gatagtattg tccagcacgt ccgcgcggaa
    19561 tcgcgagaaa tcgcccttgt agtcgctggt tccggcgact tcgagaacga aaggcgcctg
    19621 tggattctcg aacttgacga aatcaccaaa aactgaggga tcgtctgcgc cggcccactt
    19681 ccaggcggca ccgatcaccc ggtaagcgag gaaagtcccg ccggcccggg cgaagatcca
    19741 gccctgctgt tcggcgactt cgtcgaattc ctttgtggcc agataagcct gccccacgct
    19801 cccacggccg aggacgacgt tcttgtattg gacgcaatca acggtcgagg tcgccgacgc
    19861 ctcccgttcc gccggcacga ggtcggcgta gaccgcccgc cgtgtgctgc ccgagatccg
    19921 cagaacgctg ctgagcggca gggctcggga cgctccgaac agaccgtcga cctcctggaa
    19981 cgagccgaga atgaaatcgg gggtgaccca ggcgtacacc ctggagctga acacctcggg
    20041 cggcggcgaa ctctggccgg gcttcgcccg gagcaggttg tggtagcggc ctggccggcg
    20101 agccttgaac tcgtagcagc ctcgctcggg gccggccgtt gccaacgccg tgaccgcttt
    20161 gggcggcaca tatcggctgg tggccagaag cggcgccccc agcacgccat agtaatgcgg
    20221 ccggccttgg tcgaagagca tgtacatgca cgggtagctg ccgctgcatt gagcgtcccg
    20281 acggctattg tgattgaact cggcggggag tatgccgtcg tgggcgggct gataaacgcg
    20341 gcaggcgggg ccgcagagca tgccttcgag gttcagggcc gcgtgttcgg caaacaacac
    20401 gtccaagcca agctcggcga tttggcgcaa cctggggtct tcggcccact cggccaccat
    20461 gacgagacag ccgatttcct tctccacgta gcacggcgag ttgtactcca gccaaccctt
    20521 tctgatatgc tcaatgatga agagcgcgat gtgccgggac atctccgcca cggcgttgtg
    20581 gtctcggtcc agttgggcgg tcatcagatt ccacgtgtgc cccatgaagg catggttttc
    20641 cgtggccccg aattcccaaa tcgagggatt gattcttcgc ggtgccggca aggcatgcat
    20701 gatgtcgagc agacgcttcc ttgcagccgg gctcagcgaa ggatcatcgc ggaaaaggta
    20761 gtaggcgtga atggcctcgc atacccgcat atcgggatcg atcttttcgt ccaatggcat
    20821 ggcggcgaag aaggcgtcgg cctttgagac ctcctcgcga ttgcgtttca gatgcatggc
    20881 ggccagcgca cccgaccagt tcggctcgat ctcgtcgaat ttccactcct cgtgtttcca
    20941 ctggatggga tggcctgcag ccgcccagtc gtcagcgaga cgcccaagaa actcgcgttt
    21001 tcgcttttca acggccacgg ctgaatcaat gtcggggctg gtcgccgcgc acacgcctga
    21061 ggtacacgcc gcgatcaaca gcgatgcgca aaccgtgcgc cgggccggag tctgccgaat
    21121 caggttcatc attgcgagct ctcccgtcgt tcagggcgca atcgccgatc gagatgacgc
    21181 atggagaata ctcagattcc gtcgcgcgtc cagtcggtgc ccagcgttga acggcggagt
    21241 gagccggctg catcgggtcg ccttctctcg taacccaggt caataatcca tgaccagacc
    21301 cggacgacca acgaagcccg atgtgcgaat cacgaacgga atccgaggtc tgccttggtg
    21361 ggatccgccg gctacgctca ccacagcaag caggacggtc gaacttgtgg cgaacgcttc
    21421 tgtttggcga caacctgcgt aaaccccaag caacaccgac cacgtccttt gagatgaggg
    21481 cgcggtcggt gatcgactcg atggttgccg gcacaggctc gacagaccgg tcgtttgcgg
    21541 ctttacttca gcaacaggta gtcgaggccg aacatgtaat ccttcttggc cttgctgttg
    21601 gctccgacga tttccgccgt caggaggttt cggccttcct tgaggtcaaa cgttccgagg
    21661 tccagctcat cggtccggcc cacgccgtcg gcctgaaaga ggtcgatggg gtcgccggcc
    21721 ttctgaccgt tgacgtaaag ctgaacaatg ccgtaatcga cggctttggt cagccggacg
    21781 atcacgtgtt tgctgccggc ctctttggcc tggaacgcca gcgtcaattt gtcgccgggt
    21841 ttgccctggg tccaccacag atggttttca ctgctccatc cacccagttc ctgcgtgctg
    21901 gtggcgccgg ttttctcgac cacctccatt gtttcgcctt cgatcgcgcc cggcaccttg
    21961 ttgatcggca ggacgggcat gggttcgatc ttgacgtcgt ccctggtgat cgggccgaag
    22021 aaccagtcgt cccagcagcc cgacttggcg taccagtagc tgatcgcggc ccgcgtcgtg
    22081 tcacaaaccg ggttccagtg ccagttctcg atgtcgaact tgaacgactt ggtgaatgga
    22141 atgtcatcga tgatgtggaa ccggttcacg ctggtgttgc cgaagttgcc cgggccgtcg
    22201 catcgcggct gattgtggta ggcgtggacg aaccgctcat tgcagcacca ggcgtagccg
    22261 tagtaatcct cggtcccggt gccgaaatgg ctgggaaacg tctccccgtc gacatagatc
    22321 ttctcatcgc cctcgcccca ccaatccttt accgggttga tgatgtgcag tgagccgccg
    22381 acgaaacggc cgtggccgct gcaagccagg tggccccagt cggtcatcgg ccggctggga
    22441 aggttcttct cgatccgcca tttggcgtgg aagagcaagc tcttgtcgtt ccactcccag
    22501 ggcatcacgg cgaccgcgcc ggacacgcgg acttcctgct cgccgaggtt cttgatcctg
    22561 atgaccgcct tcttgttgaa cggcatccac caatggcacc agaggtcctg cggcttgtcc
    22621 ttggtaatgc ccagcggcag cgaatcgtag ggcgtcaggc cgggtgcggt gccgaagaag
    22681 tcgccgatgg gggattcgac ggtcttttcg aggacaggct ggccggtctt ttcatctttt
    22741 ttgtcgccat ggaaactcat gtagaggaca atctggcggg cggcggcgtc gagatcaccg
    22801 gcatcgaggc ggacgaggaa gccgcagatg gccttcgggc cctccatctc ccagagtacg
    22861 gcctctttat cgggagcgag gctgacctcg aaggggctct tggttcgctc gtccggtggg
    22921 cccccgccct ggcgggggtt ggacaacctg tcggccacat ccttaatctt gtcggcgagt
    22981 ctctgggtgt cttccatggt gaaggtcttg acctcggtgc cggccggcca ggtgcggtag
    23041 ttgacgtgat agtaaaagcc gccttcgtcg ctggtcacct tgcagtgttt ggcgtaggcg
    23101 atggggaagt agaggttcca cccgcgcgac cgctccccgg caatcggctt gggaaagccg
    23161 ggcacttcgc cgcccagcag tttggtcatc ggggtctcga tagccggctt gtcggccttg
    23221 tcgaggtaga tccgcagggt gcccttggga ttggctgacc agatgcgaac gattgctccg
    23281 gggccggggg tgtccatcat gacgtgctcc ttgcgaccgt ccttgtcctc gattcgcagg
    23341 tattggccgc agtcgccgtt ggcgaagtaa ccctggaagt cgctgtggct cttggaggct
    23401 cggtcccaac tcgagaactg ctttgtgaca tacggcggtt cgggcagggt tgccaatcgt
    23461 ctcaggtcgg tcatctcgtt gagcagcgat tcggtatcga tcgcggcgat ggccgacgag
    23521 aaccacacgg tcgtcattgc gagtgccagt aaggctttca tggatgtggg tttcatcttg
    23581 gctctcctgt ttgatgaagg tcatctgtcc ggcaagccac tgcggcaaag gggtattgtg
    23641 gccgggtcgg gcaaaaaggc aagtagcgcg gcaacgactg tctggaggcg ggctcgggag
    23701 gggcacgact tcggcaaggt catgtggatc ccaagggcag gactgatagg agcacttgtc
    23761 aggacgaggg gcgagtctgt ttggcgcggg cacgccgcgc ggagaacgtc ggaaggaggt
    23821 ttaggagatt gaagatcagt ctgccgcgtg tctggcgcgc atgtggcaca caggttcctg
    23881 gatcacaatc cgttttcgtt ccggcccgct gaaagtcggt ctgcttcagg tcgttcttca
    23941 gtaacgccga ctcggctaag ccggccggct ttgccgcggc gagcgtcccc ctcggcgttt
    24001 ttccccagta gcgccgacac gcccgtcggc gattcctgcc actatgaccg cgccgatgat
    24061 agaacatccg gagtgttatt cgtgtagtct ccatggcttt gcgatcggtt catgaaagga
    24121 ggcgccatgt ctgacgacac cacgacgttc cttggggagt atgggactgt attgacggtt
    24181 acgattcatc aagggcgtat cgcatctact gtgccagtcg tcagggttct gttccatcca
    24241 ccgttggctc agcacggccg cccattgttg cggtgcctca tcctcgctga gttgggacag
    24301 tctcttgccc aggcaacaca cttcaggaat ccggtccaag cccatcaact tgccccgtgc
    24361 tcccggggtc tcgtactgaa gatcgctttc ttcggccacc tcatggcggg gacggccgag
    24421 atcgagccgt cgttgggcct ggccgacgat ctcagcggtc atgaccgacg aaccccgacc
    24481 ccgccggggt cgatagaaac cgtggattcc ctcatgggaa aaacccgatt aacggccgag
    24541 atcaggaggt ctgagccttg acctgtgccc ttgttaatcc ggtagcgcgg cgctcgcttg
    24601 ggtttggcgg agacgaccga cctcgtcgca ggtggtacga ctcgcatttg gcccttggtt
    24661 acggccgctc cgccggacgg tgggtcttcg caactcgcga agtcagcggg aattacgtca
    24721 actggagccg atgagattcg aactcacgac ctcttgcatg ccatgcaagc gctctcc
//
LOCUS       DAOTPW010000058        24760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2100_length_24760_cov_5.607529, whole genome shotgun sequence.
ACCESSION   DAOTPW010000058 DAOTPW010000000
VERSION     DAOTPW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 24760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24760
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_24760_cov_5.607529"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..467)
                     /locus_tag="P5316_07200"
     CDS             complement(45..467)
                     /locus_tag="P5316_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRR84781.1"
                     /translation="MLAMHMANELLTPMVAWAFIVVAAAVLVYSSWRTGRTFDPAKVP
                     LMGVMGAFVFAAQMINFPILPGTSGHFSGGVLLAILLGPHAATLVMASILIVQCLIFQ
                     DGGLLALGANIFNMGVLPCYLGYALFALIAGARPGARP"
     gene            complement(594..1592)
                     /locus_tag="P5316_07205"
     CDS             complement(594..1592)
                     /locus_tag="P5316_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HRR84782.1"
                     /translation="MFKVRITDYLTPPVRIEENALASLATVECLMSLGPDDLIGRVED
                     SDGLIVYHQTSIPRRVIDVLDRCKVIVRGGVGFDSVDLEAAGRKGIIVCNVPDYGVDE
                     VADHAIALMMACNRGLVLADRRLRSRLEPWDYRSAEPVFRLSEATLGIVGLGRIGTAT
                     ALRAKALKMRVLACDPYLRPGIEKAVGVERVDLDTLLATSDIVSLHVPLTRETYHMIN
                     ATTLGRMKPGAFVINTARGDVVNVDDLADALESRRIGGAGIDVLPAEPPPANLRLIKL
                     WRRTEEPFVNLIITAHCAFYSESAFVEVRTKAAQEVARVLRGEKPLNCVNSEYLMR"
     gene            complement(1605..2645)
                     /locus_tag="P5316_07210"
     CDS             complement(1605..2645)
                     /locus_tag="P5316_07210"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRR84783.1"
                     /translation="MKAARLTGPNQIEYLDVPQPVCADDEVLVQVKCVGVCGTDAEMY
                     RGTMAYFKMGWTQYPITLGHEWSGVVVERGRKVTEFSVGDRVTGDVTIGCHRCENCLR
                     GSYNLCLSKIEVGLCRGKDGAYAEYLTMPARHTFRVPDTVSFEEAAMTEPAATVVKAI
                     RKAPFEPGAVCVILGDGPIGLLGLQAAEACGAGKLVLTGTVDAKLQLGHELGADVVVN
                     VRRQDLQEVIEDLTGGVGADYVMEASGSVEAMQQAVAIARMGGIVSVVGLPEIPVPEF
                     DMGNVAVRDLNIITSVASPNVFPQTLRLMAKSKIRTRPLISHELPLSETVKAFDLQIN
                     HPAERLKIMLHP"
     gene            2852..3289
                     /locus_tag="P5316_07215"
     CDS             2852..3289
                     /locus_tag="P5316_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84784.1"
                     /translation="MHKTYTWEYRIDLSPYDKLVGVVEAFFCTCPAGDYTCEHREAYK
                     LRFRRGAWRKTLLGLGRWQPVALVKGQFNQWPVVVHALIRPSPQTYRVMLRYELSLPD
                     GVRELQPEVQASVAQHIRRELEDLSAYLAECAGQPTPPEVVAV"
     assembly_gap    3609..3618
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3619..3937)
                     /locus_tag="P5316_07220"
     CDS             complement(<3619..3937)
                     /locus_tag="P5316_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HRR84785.1"
                     /translation="MPQAIRITIGEMVFDAELNDSGTAARILDALPIDGEVNRWGDEI
                     YFTIPVEGDRSDSMRTEMSVGELAYWPPGAAFCIFWGPTPASKGDEPRAASNVVPIGR
                     VIGD"
     gene            complement(4186..4857)
                     /locus_tag="P5316_07225"
     CDS             complement(4186..4857)
                     /locus_tag="P5316_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HRR84786.1"
                     /translation="MSSRTSQTLRAFYDAMYNALGPQHWWPAQTPTEVIIGAILTQNT
                     AWRNVERAIDNLKRAKALDWKRLHELSLEELAELIRPAGTFNVKARRLKSFIQWFHDR
                     FDLDLDRMFEMSVSSLREGLLEIPGIGRETADAILLYAGQKPSFVVDAYTARILYRHH
                     LIDRSADYDEIKELFESNLPDDVKLFNEYHALLVDVGKKHCRPRARCEGCPLRRFEHD
                     AERED"
     gene            complement(4862..6430)
                     /locus_tag="P5316_07230"
     CDS             complement(4862..6430)
                     /locus_tag="P5316_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR84787.1"
                     /translation="MMMLTTCRRLLQVWLIVSLMVSIGWSAVPTTWPGDSVTGPYHSR
                     LFSRPATHPAGLPAWLLASRPTTASAPASRPAVKRAIPSGPNFILITVGNQSAGMTGF
                     EGHPQIKTPNLDRLAGDGLCFQRCYTPTPMSAASMASILTGRYPHSHGVVTDKSALAS
                     SSDTFTAQLQAAGYRCGIVGKWNLPGTTAKAPGFGLTDFVATQDVDAEGHGWKWEQCP
                     VWVQGEKKTADKFLTDWEADRAIEFIETHKEQPFFLWLSFRTNEEPLTRPPGTENEYP
                     PDAMNLPDPKTKTRSDQPPSLQNVAATKPKNDRELREAKSKYAAMVSRMDENIGRLAA
                     RLDELHLREKTVVIITSDGGFALGEHGLYGRGPFIYDVMVRCPLVVRYPKLVPGGGRC
                     ARTVSLVDLAPTICELAGLPRSPMMHGHSLVALLRNPESNALPDERFIEYDAQDSGRS
                     ILIRGLVTSQYKYARYLRQDNNYDVLYHLTHDAEEMNNVAKKPDLARHYAGVVKVLQN
                     RVEHFRKVTRDQAR"
     gene            complement(6577..7728)
                     /gene="dprA"
                     /locus_tag="P5316_07235"
     CDS             complement(6577..7728)
                     /gene="dprA"
                     /locus_tag="P5316_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRR84788.1"
                     /translation="MDPSEVASSAAIEHLRWSLTDQVGPITFGRLIERFGSAQAALGA
                     GVRQLESIEGIGPATAEAIARSRDRADVACEVALAARHGVRIICRDDEEFPAPLRHIP
                     DPPICLYVRGRLEPTDALAIAVVGSRRSTLYGREQAYRFGYQLAQRGMTIVSGLARGI
                     DGESHKGALAAGGRTLAVLGNGLASVYPPEHRELADRVAADGALISELPMTTPPDTGN
                     FLPRNRLIAGLSLGVLVVEAARRSGALSTAARASEYNREVFAVPGRVDSDYSVGTNAL
                     IRDQHAKLVTCADDILDELGEVGQTLRGTDEETEAEGEPNLFHVQPTLSGDEQAVCAK
                     LDHEPRSVDQLAAAAGLPESRVASTLIMLQLKGLARQLPGNLFVRPGKR"
     gene            complement(7815..9128)
                     /locus_tag="P5316_07240"
     CDS             complement(7815..9128)
                     /locus_tag="P5316_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84789.1"
                     /translation="MNSRSDRINRRQFLEGTLGGMALASMSRAQAPGLETLGANERIR
                     LGCIGTGGQGTHHIGQWAKMNDVRIVAVCDVDQARLANAAKVAGSSPDMTKDFREVLG
                     RKDIDAVCIATPDHWHTPLAIRACQAGKHIYVEKPISHNVREGRAIADAARKAGVVVL
                     HGTQQRSSSHWGNAVRRIKDGEIGKVNMVHAWNAWATEQMFGNIGRPDDAADPPPGVD
                     YDMWLGPAPKRKFNPAHFHGTFYFFWDYAGGMISDWAVHLFDIVMWAMGYGFKSVSTI
                     GGKFVHDDMRETPDTASAVFECSGYNLYYSMRHGNSWKPHGKLDHGIEFFGTEGTLQI
                     DRMGFQIYDEQNSAQRKPRYSEEDKVDDSFEHKRHFLDCIRSKATKPRCDAEEGHKAC
                     IYGHLANIAYRVGRRIEWDAAKETILSDPEAEKLLARTYREPWTL"
     gene            9327..11546
                     /locus_tag="P5316_07245"
     CDS             9327..11546
                     /locus_tag="P5316_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR84790.1"
                     /translation="MSSAHKSASIQAATGHITVLVSLIVCAGARLAWAQTALLRMNEH
                     YIAGPMPGQNRDEWLTAAREYRRQIRENGFARAETRAAGARQLDLRIYDRADLQWMAR
                     NFACHFTFMYDRSFYDPESGRYTVDEFLDDGVREFGGYDSIMLWQGYPRLGVDDRNQF
                     DMYRDMPGGLKGLREMVRRCHERGVKVFIDYNPWDTGTRREGEPDEQALADLVAAIEA
                     DGIFLDTMTAGSVELRRRIDQARPGVAFIPEGHPAIEQLSICSGSWAQWLVDSYLPGM
                     LHLKWIEPRHMQFQIRRWNTDHVAEIETAFFNGSGMMIWENIFGSYNPWSARDRAIWR
                     RASAILRCFASDFASKRWEPFYPTLVDRLHAHRWPGDGYWLFTLLNHGQPLNNAGLID
                     VPYGKDNRFFDVWRGRDLQTRPAGTGTRIIDSVDRLGCILAIEQARITPELEKLLSQG
                     RIESEPRLPIDQRNVVRSVVEAEPVKPTPLVPRHSPPKAMVLVPGAKLRMQIKHMRRE
                     CGCYPDPGTPKDRWNDFLAGNPHDQILTHDYAVDLKSFFIDEVEVSNADFSRFLEATR
                     YQPKHPENFLKHWPNGHMPETLADHPVVYVDLDDARAYAKWAGKRLPTEAEWQLAAQG
                     TDGRAWPWGNEFDASRCNCDGQGTMPVRSLPDGRSPCGCYHMLGNVWEWTESARDDGH
                     TRFVMIRGGSWFDAKGSIWYIRSATERCDSHVKFIRMWPGLDRCATIGFRCVMDVAE"
     gene            11782..12441
                     /locus_tag="P5316_07250"
     CDS             11782..12441
                     /locus_tag="P5316_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRR84791.1"
                     /translation="MSSDSLPGGVIFDMDGVLLDSEPFICKAACMMFAERGLTVKPED
                     FIPFVGAGENRYIGGVAEKYNFPVDIIEVKRRTYEIYLEIIKGQLEPLPGVHEFLAAC
                     RKAGKKMALATSADYVKAEGNLREIGLPMSTFDAVITGDDVVHKKPHPEIFTTSAQKL
                     GLDPRACLVVEDAVNGVAAAKAAGSKCLALTTSFSREALAGADWFAPTLAEAPPQVLR
                     W"
     gene            complement(12643..13914)
                     /locus_tag="P5316_07255"
     CDS             complement(12643..13914)
                     /locus_tag="P5316_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRR84792.1"
                     /translation="MAKRRPGRLIVKSVLSHGLLIALSVVFLIPFVWLLTSSFKSESH
                     LFTKRPEWIPRGEYVRLDDGTWAEVVMQEVLEYHPVDYDEQGRPKDRRPKTTRVTLVD
                     RRGDPLLKKLTAADLPADVRDMDMGIIHPALKAAFTQSGKSLPELPRLKPVWKGRKWE
                     IPAGEQTYILKREGDRIGVYSDEPARMVIDASRIETRVWPTFRHYVKGIEGFHFSLFL
                     QNTLRIAVFTVLGTVLSSSWVAFGFSILEWRGRDKLFYLMLGTMMLPPQVTMIPVFVI
                     FKSLGWCNTYLPLIVPSFLGNAFFIFLLRQFFLTIPKDLIDAARVDGCSTFRIYWQIV
                     LPLSIPALATVALFTFMWSWNDFMGPLIYVVDEAKYTLSLGLAMFKGRFASRYGEMMA
                     VSVLLTLPIIVLFFFTQKTFIQGIKTSGMKN"
     gene            complement(13916..14893)
                     /locus_tag="P5316_07260"
     CDS             complement(13916..14893)
                     /locus_tag="P5316_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRR84793.1"
                     /translation="MAEFWTRAERKRQILGLVFAGPWILGFLVFALYPVIASFYYSLC
                     DYNVFDPPVFNGGRNYVELVREDKLFWTSLWNTGYFMFFAIPTTMLTALVLAMLLNLK
                     VRGQAFYRTIFFLPSIVPIVASTVLWLWILNPRYGVANKILGPISPYLQICWDALREK
                     LISWGVENVPALRFPPGWLTNEYWSKPGLILMSVWGVGNNMILYLAGLQEVPKELYEA
                     AEIDGASAWKRTWHITLPMISPILFFTLVMGMIGTFQYFTQAFIMTDGGGGPNDSTLF
                     YAMYLFNNAFIYFKMGYASAMAWILFVLIVICTALTFRLTRGRVYYAGA"
     gene            complement(14984..16375)
                     /locus_tag="P5316_07265"
     CDS             complement(14984..16375)
                     /locus_tag="P5316_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRR84794.1"
                     /translation="MKNVLLAIAALAVVMYTFYPLESRWKASSPDVTVLQFWHPWTGQ
                     YADDLAEVVAEFNRTHPKIQVNALFMPTGAGENMKFFVSVAGGVPPDVVFVDGAQVAS
                     WAHMGVLRPLDDLLASAGVDKSDYFEPSWKQCEYNNKVFAVPAAADPNFALVWNKDHF
                     RQAGLNPERPPQTIEELEEYALKLTIRDKKGEIERLGFLPTFVPHGSIAVLTWGWAFG
                     AEFYNDETQEFTCDDPRAIEAMKWILRLQQAYGGQERILNFEAGFGFSAQEPFIQRDL
                     SMSIAYIAYAQDIVRFAPDLDLGIGPMPVKNGVKLGCEWIGGWTMAIPYGQRGHEKEA
                     FELIRWMCADPVGTRHVALTMKLLPAYRESPFFTKDLAEDLANRPDIRLMKAFYEILK
                     RAQRVRPVSPVSAYYMTELMRALGRIRNGSMTPEEALKEARERVTEEWHKSQKRAQAR
                     HVRATANSPPPKP"
     gene            complement(16718..17227)
                     /locus_tag="P5316_07270"
     CDS             complement(16718..17227)
                     /locus_tag="P5316_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84795.1"
                     /translation="MPARTDLPQSKTRARRWWAIRSSTALIFMAAALCLLVIWNRDRN
                     LARRLLASLDPVVKELQDRTDALGVLPAHVPDPAPIGQARFSFAYTFSADERQFAIDS
                     GEPCIMAYTSLIRLLLHRDGRCVICHHKGQISAVWMAESDFQQQMLRQNQLVEEFQAK
                     RRSEPVKLP"
     gene            17816..18253
                     /locus_tag="P5316_07275"
     CDS             17816..18253
                     /locus_tag="P5316_07275"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HRR84796.1"
                     /translation="MDAFETVSLTDLLAGSSKKYFSLREANRSIILVRRIVADIVRDY
                     QELCELHAACKAYDAAGDIHRAEQGRERYARITDHISELQEELEKIGCEIKDYRLGLV
                     DFPALLDGRQVYLCWQIGEDRVSHWHEATTDDTQRRPISREFV"
     gene            18613..18954
                     /locus_tag="P5316_07280"
     CDS             18613..18954
                     /locus_tag="P5316_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84797.1"
                     /translation="MKKTVVVTLGLAICLGSGCACRETARVAPVCQPAVRPAPSRTEL
                     AAGLLFDRRPGLYDASEFTIRGDWPSTVSFWSPGQVIYAREHLIDVQGPRWGGHSYQR
                     FHTERVMVGHR"
     gene            19947..20558
                     /locus_tag="P5316_07285"
     CDS             19947..20558
                     /locus_tag="P5316_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRR84798.1"
                     /translation="MVASPIAPSPPAPKPTPTVESDLAKTKREHVIQPGDTFSGLAVK
                     YFGHAKHADLIQNANPDRDPRKLRPGMKIVIPPNPDAVSGSAVAPSPAPSAVPSPTPS
                     PLRTGAEPAKTPDLSLMLSARRLPSPEPAAAERAYTVKAGDSWESLAKRFMGTTNWTE
                     LFEHNKERLRGDRRNLRPGLVIELPEGANLSALNTTATRPASR"
     gene            21055..22257
                     /locus_tag="P5316_07290"
     CDS             21055..22257
                     /locus_tag="P5316_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRR84799.1"
                     /translation="MRPNRTFLLALPLGAAFYGTAIAGAADLPRTRIKWEKVSLVDNL
                     NEGIDVVDVNRDGKLDIISGPLWFEAPNWTPHPVRDVGINNKEFFENNGDHAIDLNGD
                     GYPDVISASWFSDKVYWYENPGKEGLAEGRAWKQHFIVDGQNTCEGTLLQDIDGDKVP
                     ELILNHWTAEKPMTIIRVKLGRNGAAPQFEKVEIGKPQTGHGIGVGDINGDKRSDIVL
                     PGGWFEQPSSDWAEKPWPFHKHNGFKMDHSSVPCLVVDVNGDGRNDIIYGKAHDYGLF
                     WMEQGPAKDGEPTWAQHEIDKSFSQVHCLVWTDLDDDGRKEIITGKRYRAHGDGDPGA
                     ADPQCLMRFIWNDKTKSFDKDVISYGEKIGSGMQIRVADLDGDKKLDLAVAGKSGTYI
                     LFNRGPNK"
     gene            22318..23424
                     /locus_tag="P5316_07295"
     CDS             22318..23424
                     /locus_tag="P5316_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR84800.1"
                     /translation="MANDKKVRVAIIGLGFGAEFIPIYQKHPHAEMYAICQRNREKLD
                     QIGDAFGVAKRYTSYEDVLNDPDVDAVHINTPIPDHAPMSIAGLKAKKHVACTVPAAT
                     SVSDCRKLVEAQKKARRNYMMMETVVYSREFLFVKELYETGVLGRIQFLRGSHQQEMA
                     GWPGYWEGLPPMHYATHCVSPCLCLAGKLAESVVCHGSGRISPKLTRKYGSPFAVETA
                     TIKLKNSNLVCEVTRSLFETARQYRESFDVYCDKVSFESPQIEHEPAILHRGEQPERV
                     TIPDYAHLLPESIRPFTTKGVYDLEQNVHLSFKQGSGHGGSHPHLAHEFITSIIERRP
                     PMPDVKTSVNWTMAGICAHESAMKGGARVKIPQF"
     gene            23676..>24760
                     /locus_tag="P5316_07300"
     CDS             23676..>24760
                     /locus_tag="P5316_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRR84801.1"
                     /translation="MQSGPAIPELILLLLIGSALIVGVIFIFRGIRGVPVLSDPRCTR
                     CGYDLRWIKPDVNVVCPECGSDLTVRRAIRFAEYQRRPRSILVGLGLIVAVFVVPVGL
                     RLVLMRSRVGPMTPASRASLPSSTLIANLATTASQPWDWQELERRLNAGQLSKRDTAV
                     AIDQLIKHMQTKPSGWREPLHWCDRFVAAADKAGFISDEQLVRLGQAFYGAQPDVKAR
                     SRLRQGQPFTFELSYGTGWSLPGLKLVMALSRTRLEGAGELGVSDPFRDDRKPADPDD
                     LSGGGPWSMIRGKVVSDISAGKHNLVFEVDTGLIDEFRPFKTDNSMKPGQAERWPKTR
                     CRWNTTVTVPVEIVAPDRPAVDLVIDE"
BASE COUNT         4934 a         7606 c         7495 g         4715 t
ORIGIN      
        1 ttttttacga acaaagcgag aaaacctcgc cggagtatgc ttgatcaggg tctagcgccg
       61 ggcctcgccc ccgcaatcag ggcgaacaac gcatatccca aatagcaggg caacacgccc
      121 atgttgaaga tgttcgcgcc aagggccagc aggccgccat cctggaaaat cagacactgc
      181 acgatcagga tggaagccat caccagcgtg gccgcgtgtg ggccgagcag aatggccagc
      241 agcaccccac cgctgaaatg cccgctggtg cccggcagga tgggaaagtt gatcatctgg
      301 gcggcgaaga cgaaggcccc catcacgccc atcagcggca ccttggccgg atcaaacgtc
      361 cggccggtgc gccaagagga gtacaccagc acggccgccg cgacgacgat gaaagcccac
      421 gcaaccatgg gcgtcagcaa ctcgttggcc atgtgcattg ccagcatgcc ggcgtgtctc
      481 cgccgcgcgc cgtcttcaga atggcggtat tacggcttat gagattgtag cactgcaccg
      541 gaaccccgtc aacggctcgc aacgtaagtt acgggtcgaa ccgtgtcagg tgttcatctc
      601 ataagatact ctgaattgac gcagttgagc ggtttttctc cacggagcac gcgggcgact
      661 tcctgagcgg ccttggtgcg cacctcgaca aaggcggatt cagaatagaa ggcgcaatgc
      721 gccgtgatga tgagattgac gaacggctcc tcggttcgcc gccacagttt gatgagccgc
      781 agatttgccg ggggcggttc ggcaggcagg acatcgatgc cggctccccc gatgcgccgg
      841 gactccaggg catcggccag atcgtccacg ttgaccacgt cgccgcgagc agtgttgatc
      901 acaaaagcgc cgggtttcat tcttcccaaa gtggtcgcat tgatcatgtg gtacgtttcg
      961 cgggtcagcg gcacgtgcag agaaacgata tcgctcgtgg cgagcaacgt atccagatcg
     1021 accctttcga cgccgacggc cttttcgatc cccggccgca agtacgggtc acaggccaga
     1081 actcgcattt tcagcgcctt ggcacgcaaa gcggtggccg tgccgatccg ccctagacca
     1141 acgatgccaa gcgtcgcctc gctcaaccgg aagaccggct cggctgagcg gtagtcccaa
     1201 ggctccaacc gcgaccgaag acgacgatct gcaagcacca atccccgatt gcacgccatc
     1261 atcaaggcaa tagcgtgatc ggccacctcg tccaccccgt agtccggcac gttgcagacg
     1321 ataatgccct tcctgccggc ggcctcgaga tccacggagt caaagccgac cccgcctcgg
     1381 acgatcacct tgcaccgatc gagaacgtcg atcactcggc ggggaatcga ggtctgatga
     1441 tacacaataa ggccgtcgga gtcttcgacg cgaccgatga ggtcgtcagg tcccaacgac
     1501 atcaggcact caaccgtcgc caggctggcc agcgcgtttt cctcgatcct gaccggcggc
     1561 gtcagatagt ccgtgattct gaccttgaac atggtacctc tcgctcaagg gtgcaacatg
     1621 atcttcagtc gttcggccgg gtgattgatc tggagatcga aggccttgac ggtctcggac
     1681 agcggcagtt cgtgcgagat caatggccgg gtccggatct tgctcttggc catgagccgc
     1741 aaagtctgcg ggaacacgtt cggcgaggcc acagacgtaa tgatgttcaa atcgcgaacc
     1801 gcgacgttgc ccatgtcaaa ctccggcacc ggtatctcag gcaggcccac gacactgaca
     1861 atgccgccca tgcgggcaat ggccaccgcc tgctgcatgg cctctacgct tccggaggcc
     1921 tccatgacgt aatccgcgcc gacgccgccg gtgaggtcct cgatgacttc ctgcaaatcc
     1981 tgccggcgca cgtttacaac aacgtcggcc ccaagttcat gaccgagttg cagcttggcg
     2041 tcgacggtac ctgtcaggac aagcttgccc gcaccgcagg cctcggccgc ctgaagccca
     2101 agcaagccga taggcccatc accgaggatg acacagacgg ccccgggctc gaacggagcc
     2161 ttgcggatgg ccttgaccac ggtcgcggcc ggctcggtca tggccgcctc ctcgaagctg
     2221 accgtatcgg gcactcggaa cgtgtggcgg gccggcatgg tgagatactc ggcgtaagct
     2281 ccatccttac cgcggcacag accgacttcg attttggaaa gacacaggtt gtacgacccg
     2341 cgaaggcagt tttcgcagcg gtgacagccg atcgtgacgt cgccggtgac ccggtcgcct
     2401 actgagaact cggtcacctt ccgcccgcgc tcgaccacga cgccggacca ctcgtggccg
     2461 agggtgatgg gatactgcgt ccagcccatc ttgaaatagg ccatggtacc gcggtacatc
     2521 tccgcgtcgg tgccgcagac gccgacacat ttgacctgca ccaggacttc atcgtcggca
     2581 cagacgggtt ggggaacgtc cagatactcg atctggttcg gtccggtcag acgagctgct
     2641 ttcatgacgt gggcctcttt gggtgcggtt cttcggtccc gacctcttcc gcgataccgc
     2701 ttatacgccg gacgggccca aaatcaagcc gcctcgacac ccgtttgggc acgagctgct
     2761 gcttctttgc tttctttcct cgccgtagtc cacctccgtt atcgttctcg ctatactgtg
     2821 cggtctgcta ggatgaaggg gcgatctggc catgcacaag acctacactt gggaataccg
     2881 catcgatctg tcgccctacg acaagctcgt gggcgtggtg gaggcgtttt tctgcacctg
     2941 cccggccggc gactacacct gtgagcatcg cgaagcctac aagctgcgat ttcgccgtgg
     3001 ggcatggaga aaaacgttgc tgggtctcgg ccggtggcag ccggtggcgc tggtcaaggg
     3061 gcagttcaac cagtggccgg tcgtggtgca cgccctgatc cgcccgtcgc cgcaaaccta
     3121 tcgcgtgatg ctccgttacg aactctccct gcccgatggc gtgcgcgagc ttcaaccgga
     3181 ggttcaggct tcagtcgccc agcacattcg ccgcgaactc gaggatctga gcgcctacct
     3241 cgccgagtgc gccggccagc cgaccccacc ggaagtggtg gccgtgtagg cagggttcag
     3301 ggttcagtag ggcacggtcg aagccgccaa aggcggcgca gacctgccgc gaactctctg
     3361 gtggatttcc tcattccttt gtcccgccgc gccagatctt cgccattcgg acttcggcat
     3421 ttactgctca ctgggctttc ggcactggtc attcgccgcg ttccgttcag tccccccttg
     3481 gcagggggaa agatcaggcg cgctcgaacc ccgagcggcc agcaatgtgg tgccgatcgg
     3541 ccgcgtcatc ggcgatctca gcgaattgtc cacatcccgc gacggtgagc cggtgaggat
     3601 cgagcgcgnn nnnnnnnnga tcgccgatga cgcggccgat cggcaccaca ttgctggccg
     3661 ctcggggttc gtcgcccttg gaggccggcg tagggcccca gaaaatgcag aaagccgcac
     3721 ccggcggcca gtaggccagc tcgccgacgc tcatctcggt tcgcatcgag tcgctcctgt
     3781 caccctcgac tggaatagtg aaatagatct catcgcccca gcgattcacc tcgccgtcga
     3841 tcggcagggc gtcgagaatt ctcgcggccg tgccggagtc gttaagttcg gcatcgaaga
     3901 ccatctctcc gatcgtgatt cggatggcct gaggcatgga atggctccta tgactgtcat
     3961 ccctcgtgcg aagcagaaag cggcacgtgg ggcggcgtcg aggtcgccgc agtgaccgag
     4021 acctgccgct gagtgacgca gagtcctttg cagtgcggga cgcgttccca gcccgctatt
     4081 gacctgcaga atgccggtcc cacactatga ttgcctctgc gtttctactc aagcttcgag
     4141 gcggcaggtc tccgccgcct acggcgtctc caaccgtgcc ctacctcagt cctcgcgctc
     4201 cgcatcgtgc tcgaaccgcc ttagcgggca gccttcgcag cgggctcttg gacggcagtg
     4261 cttcttaccg acgtcgacca acagtgcatg gtactcgttg aacagcttca cgtcatcggg
     4321 caaattggac tcgaacagtt ccttgatctc gtcatagtcc gccgaccggt cgatcaggtg
     4381 gtgccgatag aggatccgcg cggtgtaggc gtcgacgaca aagctcggct tctgtccggc
     4441 gtacaacaga atcgcgtcag ccgtctcgcg gccgatgcct gggatttcaa gcagtccctc
     4501 gcgaagcgac gacacggaca tctcgaacat gcggtcgaga tccaggtcga accggtcgtg
     4561 gaaccattgg atgaagcttt tgagccgccg ggccttgacg ttgaacgtcc cggccggccg
     4621 gatcagctcg gccagctctt caagggacag ctcgtgcagg cgcttccagt cgagggcctt
     4681 ggctcgcttc aggttatcga tcgcccgctc gacgttgcgc caggcggtgt tctgggtgag
     4741 gatcgccccg atgatcactt cggtgggcgt ctgcgcgggc caccagtgtt gtggcccgag
     4801 ggcgttgtac atcgcgtcat aaaacgcccg caacgtctgc gaagtccggc tgctcatgtg
     4861 ctcacctggc ttggtcccga gtaaccttgc gaaaatgctc cacacggttt tgcaggacct
     4921 tcactacgcc ggcatagtgc cgcgccaaat ccggtttctt ggccacgttg ttcatctcct
     4981 ccgcgtcgtg ggtcaggtgg tagagaacgt cgtagttgtt gtcctgacgc aggtagcggg
     5041 catacttgta ctggctggtg accagcccgc ggatcaggat cgaccggccg gaatcctgag
     5101 catcatactc gatgaaacgt tcgtcgggca gggcattcga ctcgggattc ctcagcagcg
     5161 caacaagcga gtgcccgtgc atcatgggcg agcgcggcaa gccggccagt tcgcagatcg
     5221 tcggggccag gtccaccagg ctcacggtgc gggcacaccg gcccccgccg ggaaccagct
     5281 tcggatagcg cacaaccagc gggcaacgga ccatgacgtc gtagatgaac ggcccgcgac
     5341 catagaggcc gtgctcgcca agggcaaaac ccccgtcgct ggtgatgatc accactgtct
     5401 tttcgcggag atgcaactca tccagacgag ccgctaatcg gccgatgttc tcgtccattc
     5461 gcgagaccat ggcggcatat tttgattttg cctcgcggag ttcccgatca ttcttgggtt
     5521 tggtcgcggc gacattctgc agcgatgggg gctgatcact gcgtgtcttg gtcttcggat
     5581 ccggcagatt catcgcatcg ggcggatact cattttctgt tccggggggt cgggtcaacg
     5641 gctcttcgtt cgtgcggaat gacaaccaga ggaagaacgg ctgctccttg tgcgtttcga
     5701 tgaactcgat cgctcgatcc gcctcccagt cggtcaggaa cttgtcggcc gtcttcttct
     5761 caccttgcac ccagacaggg cactgttccc acttccatcc gtgaccctcg gcatccacgt
     5821 cctgcgtggc aacgaagtcc gtcaggccga aaccgggggc cttggccgtc gtcccgggca
     5881 ggttccactt tcccacgatg ccgcatctgt agccggcagc ctggagctgg gctgtgaatg
     5941 tatcgctgct cgatgcgaga gcagacttgt cggtgaccac gccgtgcgag tgcgggtacc
     6001 ggccagtgag aatgctcgcc atgctggcgg cgctcatcgg cgtcggcgta taacatcgct
     6061 ggaaacacag cccgtcacca gcaagccggt cgagattggg cgttttgatc tgcgggtggc
     6121 cctcaaaacc ggtcatgccg gccgattggt tgccgacagt gatcagaatg aagttgggac
     6181 cggagggaat ggctcgtttg accgcgggcc tgctggcagg ggccgaggcc gtcgtcggtc
     6241 tgctggcgag gagccaggca ggtaagccgg cagggtgtgt cgcgggcctg gaaaacaacc
     6301 ggctgtgata gggtccggtg acggaatcac ccggccaagt ggtcggcacc gcactccatc
     6361 cgatcgaaac catcaaagag acgatcaacc agacctgcaa caatcgccga caagttgtga
     6421 gcatcatcat atccgcgaat cctatgagcc tgctcctaag actgccatgg gattgtaccg
     6481 ggcaggcgtc ctttaggcgc gttggaacgt tggaaaccgt tggagctcag gattcccagg
     6541 cgtccagccg gccggccgcg cggtccgtca cctagctcat cttttccccg gacgcacgaa
     6601 gaggtttcct ggaagctgcc gggccagtcc tttgagctgc agcatgatca gggtggacgc
     6661 tacccgtgac tccggcaacc ctgcggcagc cgcaagctgg tcaactgatc gcggctcgtg
     6721 gtcgagcttg gcacaaacgg cctgttcgtc gccgctgagc gtcggttgaa cgtggaacag
     6781 attcggctct ccctcggcct cggtttcctc gtcggtcccg cgcagggtct ggccgacctc
     6841 acccagttca tccaggatgt catcggcaca agtgaccagt ttggcgtgct ggtcgcgaat
     6901 caaggcgttg gtgccgaccg agtaatccga atccacgcgg ccgggaaccg cgaagacctc
     6961 gcggttgtac tcgctggccc gggcggccgt ggaaagagcc ccactacgac gggctgcttc
     7021 gacgaccagc acacccagcg acaaaccggc aatcaagcgg tttcggggca agaagttgcc
     7081 ggtatccggc ggcgtggtca tgggcagctc gctgatcagc gccccgtcgg cggccacgcg
     7141 gtcggccagt tcgcgatgct cgggcgggta gacgctggcc aacccattcc ccagaacggc
     7201 tagtgtacgt ccgccggcgg ccagggcgcc cttgtgcgac tcgccgtcta tcccgcgggc
     7261 caacccggag acgatcgtca tgcctcgctg ggcaagctgg tagccaaagc gataagcctg
     7321 ctcgcggccg tagagtgtgc tgcgacgtga cccgaccacc gcgatggcca atgcatcagt
     7381 gggttccagc ctgccgcgga catagaggca aatcggcggg tcgggaatgt ggcgcaacgg
     7441 ggcggggaac tcctcgtcat cgcggcagat gattcgtaca ccgtgccggg cagccagagc
     7501 cacctcgcag gcaacgtcgg cccgatcgcg agaccgggcg atggcctccg cggtcgccgg
     7561 gccgatgcct tcaatgctct ccaattggcg aactcccgca cccaaagccg cctgtgcgga
     7621 gccgaagcgc tcgatcaggc ggccaaacgt aatcggcccc acctggtcgg tcaaagacca
     7681 cctcaggtgt tcgatcgcgg ccgatgaagc cacctctgac gggtccatgg tcaactcgcc
     7741 tccagaccac ccccctatcg tttgtaaagg ctaacaacgg ttgccagtgg tgacaagaac
     7801 agtggccaac gcccttagag cgtccacggt tcccgatacg tgcgggcgag gagcttttcg
     7861 gcctcggggt cgctcagaat cgtttccttg gccgcatccc attcgatccg ccggccgacg
     7921 cggtaagcaa tgttggccag gtggccatag atgcaggcct tgtggccctc ttcggcgtcg
     7981 catcggggct tggtggcctt actccggatg caatcgagga aatggcgctt gtgttcgaag
     8041 gaatcatcaa ccttgtcttc ctcgctgtaa cggggtttgc gctgggcgct attctgctcg
     8101 tcgtagatct gaaagcccat tcggtcgatc tgaagtgtcc cctcggtgcc gaagaactcg
     8161 atgccatggt ccagtttgcc gtggggtttc caactattgc cgtggcgcat ggagtagtag
     8221 aggttgtagc cggagcactc aaagactgcg ctggccgtgt ccggggtctc gcgcatgtca
     8281 tcgtggacaa acttaccgcc gatcgtggaa accgatttga agccgtagcc catcgcccac
     8341 atgacgatgt cgaaaaggtg cacggcccaa tcactgatca tgccgccggc ataatcccag
     8401 aagaaataga aagtcccatg gaaatgcgcc ggattgaact tccgcttcgg cgccggtccg
     8461 agccacatgt cgtaatcgac gccgggcggc gggtcggccg catcatccgg tcggcctatg
     8521 tttccaaaca tctgctctgt ggcccaggcg ttccaggcat ggaccatgtt gaccttgccg
     8581 atttctccgt ccttgatccg tctgacggcg ttgccccagt gagaactgga tcgctgctgg
     8641 gtcccgtgaa gtacaacgac tccggccttg cgagcggcat cggcgatcgc ccgtccctcg
     8701 cggacgttat ggctgattgg tttctcgacg tagatgtgtt tgcctgcttg gcaggcacgg
     8761 atcgcaagcg gtgtgtgcca atgatcgggc gtcgcaatgc agactgcgtc aatgtccttt
     8821 cggccgagga cttctcgaaa atccttggtc atgtccggtg acgagcctgc caccttcgca
     8881 gcgttcgcca agcgagcctg atcgacgtcg caaacggcga cgatccgtac gtcgttcatc
     8941 ttagcccatt ggccgatgtg gtgggtgccc tgtccgcccg tgccgatgca gccgaggcga
     9001 atgcgctcgt tggccccgag cgtctccagg ccgggggcct gagcccggct catggaggcc
     9061 agtgccatgc cgcccaacgt accctccaga aactgacgac gattgattcg gtcacttcga
     9121 gaattcatga caggcttcct catagactga cacccggggc agagcgaccc atagcctctg
     9181 cttgtcgccc ggtaatgaag gctacacccc gaaccgaagc tgtgtcaacg cctgacccgg
     9241 gccgcctcga cgtggcccgt cgcagccgtt aaggtcggga gcgggccttg tggtgcattt
     9301 ggaggaaacc agacaggaga accctgatgt cctcggctca caagtcggct tcaatccagg
     9361 cggctaccgg gcacattacc gtgcttgttt cgctgatcgt atgcgccggg gctcggcttg
     9421 catgggccca aacagctcta ctgcgaatga atgagcacta cattgccggg ccgatgcctg
     9481 ggcagaacag agatgagtgg ctcacggcgg cgcgggagta tcgccgacag atccgcgaga
     9541 atgggtttgc gcgggccgaa acgcgagcgg cgggggcacg gcagctcgat ctgaggattt
     9601 acgaccgcgc cgatctgcag tggatggccc gcaacttcgc gtgtcatttc actttcatgt
     9661 acgaccgttc gttttatgac cctgagtccg gccgatacac cgtcgatgag ttcctcgacg
     9721 acggcgtgcg cgagttcggc ggttacgaca gcatcatgtt gtggcagggt tatcctcgtc
     9781 tcggggtgga cgaccgcaac cagttcgaca tgtaccgaga catgccgggc ggcttgaagg
     9841 gtctgaggga aatggtgcgc cgatgccacg agcggggcgt gaaggtcttc atcgactaca
     9901 acccgtggga caccggtacc cggcgtgagg gcgagcccga cgaacaggcc ctggccgatc
     9961 ttgtcgcagc catcgaggcc gacgggattt ttctggacac gatgacggcc ggatcagttg
    10021 aactgcggcg gcggatcgat caggcccggc cgggtgtggc gtttatcccc gaaggacacc
    10081 cggctatcga gcaactctcc atctgcagcg gatcttgggc tcagtggctg gtggactcat
    10141 accttccggg gatgcttcat ctcaaatgga tcgagccgcg ccacatgcag tttcagatca
    10201 ggcggtggaa caccgatcat gttgccgaga tcgaaacagc gttcttcaac ggcagcggca
    10261 tgatgatctg ggagaacatc ttcggatcgt acaacccgtg gtcggcgcgg gatcgtgcaa
    10321 tctggaggcg cgcctcggcc atactgcggt gtttcgcgtc cgatttcgcg agcaagcggt
    10381 gggagccttt ctatccgacc ctcgttgacc gtctgcacgc tcaccgctgg ccgggcgacg
    10441 ggtattggct gttcacattg ctcaatcatg gtcagccact gaataacgcc gggctgatcg
    10501 atgtgcctta cggcaaggac aacaggtttt ttgacgtgtg gaggggtcgg gatttgcaga
    10561 ccagacccgc cggcaccggc acaaggatca tcgacagcgt cgatcggctc ggctgcattc
    10621 tggcgattga gcaagcgagg atcacgcccg aactggagaa actgctcagc cagggaagga
    10681 tcgagtccga gccgaggttg ccgatcgacc agcgcaacgt cgtccggtcg gtcgtcgaag
    10741 cagagcccgt gaagcctacc ccgcttgtac cgcgacactc gccaccgaag gctatggtcc
    10801 tcgttcccgg tgcgaagctg cgaatgcaaa taaagcacat gcgccgcgag tgcggttgtt
    10861 atcctgatcc gggcacgccg aaagacaggt ggaacgactt tctggccggc aatccgcacg
    10921 accagattct gacccacgat tatgccgttg acttgaagag ctttttcatt gatgaggtcg
    10981 aggtctcgaa cgcagatttc tcccgttttt tggaggcgac ccgctaccaa cccaagcatc
    11041 cggagaactt cctgaagcac tggccgaacg gccacatgcc ggagaccctg gccgatcatc
    11101 cggtggtgta tgtcgatctc gatgacgccc gggcttacgc caagtgggcc ggcaagcggt
    11161 tgccgacgga ggcagagtgg caactggccg cccaaggcac cgacgggcga gcctggccgt
    11221 ggggcaacga atttgacgcc tctcgctgca actgcgacgg ccaagggacc atgccggtcc
    11281 gctcgcttcc tgacggccgc agtccttgcg gttgttacca catgcttggc aatgtctggg
    11341 aatggaccga gagcgcccgc gacgacggcc acacgcgttt cgtgatgatc cgcggcggaa
    11401 gctggttcga cgccaagggc agcatctggt acattcgcag cgcaaccgag cggtgcgatt
    11461 cccacgtcaa attcatccgc atgtggccgg ggctggatcg gtgcgcgacc atcgggtttc
    11521 ggtgtgtgat ggatgtcgca gagtaagggt tcaggcggaa atgacgaatg tcgaaacccg
    11581 aatgacgaag gacagtgcca cattttgggc tgcgtcgttt ggatttcttt cgtcgttctg
    11641 gattcgtcat tcgggtttct ttggtcattg cacattcctc attgatcatt tgcccgcctt
    11701 gaccggcccc cgagcagtgg atagcttgcg ctgcggtcgc cggtcgaacc ggcgtctgcg
    11761 attgaaggac atggagacca catgagtagc gattcgctgc ctgggggcgt gattttcgac
    11821 atggacggcg tgctgctcga ttccgagccg ttcatctgca aggccgcgtg catgatgttt
    11881 gccgagcggg gcctgacggt caagcccgaa gacttcatac ctttcgtcgg agcgggcgag
    11941 aatcgctaca tcggcggcgt ggccgagaag tacaactttc ccgtcgatat catcgaggtc
    12001 aaacgccgta catacgagat ctacctcgag atcatcaaag gccagctcga gccgttgccg
    12061 ggcgtacacg aattccttgc cgcttgccgc aaggccggca agaagatggc cctggcgacc
    12121 agcgccgatt acgtcaaggc cgagggtaat ctccgtgaga tcggcctgcc gatgtccacc
    12181 ttcgacgcgg tgatcaccgg cgatgacgtc gtccacaaga agccgcaccc cgagatcttc
    12241 acgacctcgg cgcagaaact tggcctcgac ccgcgcgcct gcctggtggt cgaagacgcg
    12301 gtcaacggcg tggccgccgc caaggccgcc ggttcaaaat gcctggctct gaccacgagc
    12361 ttcagccgcg aggcccttgc cggtgccgac tggttcgccc caacgctcgc cgaggccccg
    12421 ccgcaagtcc tccgctggtg aaaggtgaac cgcagtggag acagtagata tcgacgtcgg
    12481 ttagatcatc gggctgtgcg gactcattat ttggctgctc accgcacatg gtctgcgcat
    12541 ccgtttcagc ccggtggttc cccggacatg gcgacgcagg gccgtcgcca tgccacccgg
    12601 tcactgacaa ctgatcactg aaaactgacc actcacgccg cctcagttct tcatccccga
    12661 cgtcttgata ccctggatga aggtcttctg ggtgaagaag aacagcacga tgatcggcag
    12721 ggtcagcagc acgctcaccg ccatcatctc gccgtatcgg ctggcgaagc ggcccttgaa
    12781 catcgccagg cccagcgaga gcgtgtactt ggcctcgtcc accacgtaga tcagcggacc
    12841 catgaaatcg ttccacgacc acatgaaggt gaacagggcc acggtggcca gggccgggat
    12901 cgacagcggc aggacaatct gccagtagat gcggaaggtg ctgcatccgt ccacgcgggc
    12961 ggcgtcgatc aggtccttgg ggatggtcag gaagaactgc cgcagcagga agatgaagaa
    13021 cgcgttgccc aggaacgacg gcacaatcag cggcagatag gtgttgcacc agcccagcga
    13081 cttgaagatc acgaacaccg ggatcatggt cacctgtggc ggcagcatca tggtgccgag
    13141 catcaggtag aacagcttgt cgcgtccgcg ccattcgagg atcgagaagc caaaggccac
    13201 ccaactggaa ctgagcaccg tcccgagcac ggtgaacacc gcgatgcgca gcgtgttctg
    13261 caggaacaag ctgaagtgaa acccctcgat gcctttaaca taatgccgga atgtcggcca
    13321 gactctggtc tcgatccgcg aggcatcgat caccatacga gccggctcgt cgctgtatac
    13381 gccaatccgg tcgccctcac gcttaaggat gtaagtctgc tcgcccgccg ggatctccca
    13441 cttgcggccc ttccacacgg gcttgagccg cggcagctcc ggaagcgatt tcccggactg
    13501 cgtgaaggct gccttgagag cggggtgaat gatgcccatg tccatgtcgc ggacgtcggc
    13561 cggcaggtcg gcggcggtga gtttcttgag aagcggatcg ccccggcgat cgaccagtgt
    13621 gacccgggtt gtcttcggac ggcgatcctt tggccggccc tgctcgtcgt agtcaacagg
    13681 gtggtattcg agcacctcct gcatgacgac ctcggcccag gtgccgtcgt cgaggcgcac
    13741 gtactcgcca cgggggatcc actcgggccg cttggtaaac agatgcgact cgctcttgaa
    13801 tgatgaagtc agcagccaga caaacgggat cagaaacaca accgacagcg cgatcagcag
    13861 cccgtggctg agcaccgatt tgacgatcaa tctgccgggt cttcgtttcg ccacgtcatg
    13921 cccccgcgta gtacactctg ccgcgcgtca gccggaaggt cagcgcggta cagatgacaa
    13981 tcagaacgaa caggatccac gccatggccg aagcgtagcc catcttgaag tagatgaatg
    14041 cgttgttgaa caggtacatc gcgtagaaga gggtcgaatc gttcggcccg cccccgccgt
    14101 cggtcatgat gaacgcctgg gtgaagtact gaaacgtccc gatcatgccc atgaccaagg
    14161 taaagaacag gatcgggctg atcatcggca gagtgatgtg ccaggttcgc ttccaggcac
    14221 tggccccgtc gatctcggcg gcctcgtaaa gctccttggg cacctcctgc aggcccgcca
    14281 gatacaggat catgttgttg cccacgcccc agacgctcat cagaatgagt ccgggcttgg
    14341 accagtactc attggtgagc caacccggcg gaaaacgcag cgcgggcacg ttttctaccc
    14401 cccacgagat cagcttctca cgaagcgcgt cccagcatat ctgcaggtag ggagatatcg
    14461 gtccaaggat cttgttggcc acgccgtagc gcgggttcag aatccacagc cagagcacgg
    14521 tcgaggccac gatcggcacg atcgacggca ggaagaagat cgtccggtag aaggcttgtc
    14581 cacggacctt gagattcagc agcatcgcca gcaccagggc agtcaacatc gtcgtcggta
    14641 tggcaaagaa catgaagtag ccggtgttcc acagggatgt ccagaaaagc ttgtcttcgc
    14701 gcacgagctc gacgtagttg cggccgccgt tgaacaccgg agggtcgaac acgttgtaat
    14761 cgcaaaggct gtagtagaac gaggcaatca ccgggtacaa tgcaaagacc agaaagccca
    14821 gaatccaggg accggcgaag accaggccca ggatttgtcg ctttcgctca gcccttgtcc
    14881 agaactcggc cattcctcaa cctttcgcca cggcgatgat tccactgcga ggctcaccgc
    14941 gcgtcccgga tccctcccgg caccgacttg cggccgggcg cactcagggt ttcgggggcg
    15001 ggctattcgc tgtcgccctc acgtgtcgag cctgggcgcg cttctggctc ttgtgccact
    15061 cctcggtgac ccgttccctg gcttccttca gggcctcctc cggcgtcatg ctgccgttgc
    15121 gtatccgccc cagcgcccgc atcaactcgg tcatgtaata ggcactcacc ggcgagaccg
    15181 gacggacccg ctgagctcgc ttgagaatct cgtaaaaagc tttcatcagg cggatgtccg
    15241 gtctgttcgc caagtcctca gcgagatcct tcgtgaaaaa cgggctttcg cgataggctg
    15301 gtagcagctt catcgtcagg gccacgtgac gcgtgccgac cgggtccgca cacatccagc
    15361 ggatcagctc aaaggcctct ttttcgtgac ctcgctggcc atagggaatc gccatcgtcc
    15421 agccgccgat ccactcgcaa cccagcttca ccccgttctt gaccggcatc ggcccgatgc
    15481 cgagatccag gtccggtgca aacctgacaa tatcctgcgc ataggcgatg taggcgatgc
    15541 tcattgacaa gtcgcgttgg ataaacggct cctgcgcaga aaagccaaaa ccggcctcga
    15601 aattcaggat ccgctcttgc ccgccgtacg cctgctgcaa ccgcaggatc cacttcatgg
    15661 cctcgatagc gcgaggatcg tcgcaggtga actcctgggt ctcgtcgttg tagaactcgg
    15721 ccccaaaggc ccagccccag gtcagcacgg caatcgaacc atgcgggaca aacgttggca
    15781 ggaacccaag ccgctcgatc tcgcctttct tgtcccggat cgtcagtttg agggcgtatt
    15841 cctccagttc ttcgatggtt tgcggcggcc gttcgggatt cagcccagcc tgtcgaaaat
    15901 gatccttatt ccacaccagc gcgaaattcg ggtctgcggc ggccggtacg gcaaagacct
    15961 tgttgttgta ttcgcactgc ttccaacttg gctcgaagta atcggacttg tcgacccctg
    16021 cggaagccaa cagatcgtcc aaagggcgaa gcactcccat atgggcccat gaagcaacct
    16081 gggcgccgtc aacgaatacg acgtcaggtg gaactccgcc ggccaccgag acgaagaact
    16141 tcatgttctc gcccgccccc gtgggcataa acagggcgtt aacctggatc ttgggatggg
    16201 tgcggttgaa ctccgcgacg acctcggcca agtcgtcggc gtactggcct gtccaggggt
    16261 gccagaattg cagcactgtc acatcaggcg atgaagcttt ccatcgcgat tcaagcgggt
    16321 agaacgtgta catgaccacc gccaacgccg cgattgccag cagaacgttc ttcacgctcg
    16381 ctcctcttcc tgcaatgcgc ctcacatggg tcggctggtg ctccgcgatt cacctgccag
    16441 ccgatcaggg catggtaaag catcaaccca gcgacggcaa ccacgggaag cctggcagct
    16501 cgtggcgggg ccatgatgga tcaggcggcg gccggcgacg gctccttcag gcaagtcgtt
    16561 cagaaccgca gccccggttt ctcgatacaa ccgtgcttgt ccggtgtagc agggtgacaa
    16621 ggccggaagg tgcggtgtgc tggtggcggg accgccgtgc ggtgtggccc gtcatgccgg
    16681 cggactgcgg cgaagcagat aggtagggac gccggcatca aggcagcttg accggctcgg
    16741 accgccgctt cgcctggaac tcctccacaa gttggttctg acgaagcatc tgctgctgga
    16801 aatcactttc cgccatccat accgccgaga tttgtccctt gtgatggcag atgacgcacc
    16861 ggccatctct atgaagaagc agacggatga gcgacgtgta ggccatgatg cacggctcgc
    16921 ccgagtcgat ggcaaattga cgctcgtcgg cactgaatgt gtaggcaaag ctgaatctcg
    16981 cctggccgat gggtgccgga tcaggtacgt gtgcgggtaa aacgccgaga gcatccgtcc
    17041 tgtcctgaag ttccttcacc acgggatcga gggacgcaag caagcgacga gcaaggttac
    17101 ggtcacgatt ccagatgacc agaaggcaca gggcggcggc cataaatatg agcgccgtcg
    17161 aggaacgaat cgcccaccac cgtcgggctc gggtcttgct ctgaggcaga tccgttctcg
    17221 cgggcaatct gcgcccccga gcccggacaa catgccggtc tgatgattcc atgccaggca
    17281 tgggcgtttt gctccgtgat cggctgccgc cggtcggaga tcaactcacg gatgcgccgc
    17341 accggccgtc aacactgccc gatcccacaa agaaaagccg cgatcgacag gcaatcctgg
    17401 gtatgagcat acgagattcg ctcatcagtg gaaagccctg tcgcctatac gatgtcgaac
    17461 acagccggac ctgcccgatt cgcggcatct cggaggcgca agtaagcatc gcagatacaa
    17521 gttcttgcgt gaagctcagg cagcgtaagg gaccaatagc ctcgacgata caagcgtaga
    17581 acctggttct cgtcgattcg cgtcgcttgg gccgcatgac ctgacacccg ccgagcttgc
    17641 acgcggtctc atcagcatgc agcgttgact tgccacaaga cctttgatag tgtgaattct
    17701 ggcgaccgga taaggttgtc atcgcggtca gaaagccgcg aacaatcgcg gggaactcgc
    17761 acgacattga tgccccgtat cgtcgacatc gtgtcgcggc gcgtggagtc acgcgatgga
    17821 tgcctttgag acagtctcgc ttaccgacct cctggccggc tctagcaaga aatacttttc
    17881 actgcgagag gccaaccgat ccatcatcct cgttcgacgg attgtagccg acatcgttcg
    17941 tgattatcag gaactctgcg aactacacgc ggcctgcaag gcttatgacg ccgccggcga
    18001 tattcaccgc gcagaacaag gccgtgagag atatgctcgc ataaccgacc acatttccga
    18061 attgcaggaa gagctggaga agatcggttg cgagatcaag gattatcggc ttgggctggt
    18121 cgattttccg gcccttttgg atgggcgtca ggtgtacctt tgctggcaaa tcggtgagga
    18181 tcgggtgtcg cattggcacg aggcaaccac cgacgacact cagcgccgac caatctcgcg
    18241 agaattcgtc tgataagatc ctgagaacaa ggatatgcgg ctgggggatg gaatatgcca
    18301 agcgtcggcg cgcaggagcg cgtgttcctc agcacgccgg tcaaccggtc cgcagctttc
    18361 gtgcaatcgt tagaaacctt acagcttgcc ttgttttaca gggaccccat gcaagaatgg
    18421 cgaacagcca atgaataccg gaccacgcct ttgatcgcaa cgcgggaacg ccctttgcgg
    18481 ggggccatag cgaggaggtg ggacggcatt ctgagcagaa aagggaccag ggtgttgtct
    18541 ctctgcggtg ctcggcgtat gcggccgacg atcgaggttc cgtctgacct cttgtccgcg
    18601 agggtgggag aaatgaaaaa gacggtcgtt gtcaccctgg gactggctat ctgtctgggc
    18661 agtggttgtg cttgccggga aaccgcaaga gtcgctcctg tttgtcaacc cgctgttcgc
    18721 ccggcaccgt cccggacgga actcgcggcg ggactgctgt tcgaccgccg gcccggtctc
    18781 tacgatgctt ccgaattcac catccgcggt gactggccct cgacggtatc gttctggtcg
    18841 ccgggacagg tgatctacgc cagagaacat ctcatcgacg tccagggccc gcgctgggga
    18901 ggtcactcat accagaggtt ccacacggag cgggtcatgg tcggacaccg ctaaccgact
    18961 cataagatca ggcagtttcg agatggggca cacctgccga gcaggaccag agacgcgtca
    19021 tggggtattc gtgcagccgc tgccgaccga atcctgacct tgtcgcctgc ctcgaaaccc
    19081 gctccgactt tctcccttgc catcgcccga aaagcgggag ttcggatgca atcctgatga
    19141 cgcaaacatc cctgtccctt gcaggcgtta tctgccgata tttcactttg acttgcgtga
    19201 gtccggggta attggcttgt caattcgacg gattctcgtt atagtggccg cgttaaaccg
    19261 tgaataatgc ccgagacagc agtgaacggc ttccggtggc cccgttcgac tgcttaatgg
    19321 ccgcaggatt tccggcgggt ccttgagtgg cagcggtatc gttcttgaag tacatgaaca
    19381 gggttaggga atcgcgaaca ggccgaccca tcagtgcggg gttcatagga tagagagggg
    19441 tccgttgagt cgcttcgaac ggcaattctc agaggtgtag aacaatgcaa ctggtaacca
    19501 aggtagccat tggtgttgga gtcgttgtga tcggtctggg aacctactac gcggccactc
    19561 ggatgggtac aggcaagtcg caagaaccgg aaatggctcg tgctctacag gacgatgatc
    19621 cgttggctgt cggcccgtcc acttcccgca gggcggctga cacgcccccc cggcaggggc
    19681 agcggacgcc ccaaaccggt gtcccatccg gcgtcagtgg aaggaagccc cggatctcag
    19741 aggcggacac cccgccgcga ggctctgcaa cccgccccga acaggcacgt ccgcttgtcg
    19801 gtgatgcccg gccaacaaca ggttcgacca cccgccccgg ggggatactg cagccgccga
    19861 cgaccggtcc ggccatcgcc gatcgcccgc ctgacacagc accctcgatc gtgacggggc
    19921 cgctgactcc accaatcgag tcgtcgatgg tagcttcgcc catcgcgccg agtccgcccg
    19981 cacccaaacc cacgccgacc gtggaaagcg accttgccaa gacaaaaagg gaacacgtga
    20041 tccagccagg cgatacattc agcgggctgg cggtcaagta ctttggtcac gcaaagcacg
    20101 cagacctgat ccagaatgcc aacccggaca gggatccgcg caaacttcgc ccaggcatga
    20161 agatcgtgat cccgcctaac ccagacgcgg tctcgggtag cgccgtcgca ccgtcaccgg
    20221 caccctcggc cgttccctcg ccgacgccct ccccactccg taccggagcc gaaccggcga
    20281 agacacccga tctgagcctg atgctttccg ccagacgcct tccatcaccc gagcctgcgg
    20341 cggcagaacg agcctacaca gtcaaggcag gtgacagttg ggagtccctt gcaaagcggt
    20401 tcatgggcac gaccaattgg actgagcttt tcgagcacaa caaggaacgt ctcagaggtg
    20461 atcgacgcaa cctgcgaccc gggctggtga tcgagctgcc agagggcgct aacctgtcgg
    20521 cgctcaatac gaccgcaact agaccagcct cacggtgacg cgacccgtac cgcgccatgc
    20581 attcttgttc agttcaagca ggcaaggaca tggggcagaa ccgcatgcag ggataggcgc
    20641 gcttggcagg gcctttcacc caggcagacc gtttgttcgg ccaacgcccc agagccgccc
    20701 tgcaaggtgt tgaaccgggt atcgtcaaac tcgacagtct cccttcctgc caattcggcg
    20761 ataagcatcg ttcctcgaca taagtcattc caaaatagta gcttacaacg agagggggag
    20821 ctgtcaaagc tctgagagac ggggctcccc ttgaatcgcc tcggcggtac ctcgtggccg
    20881 agctgccgct cgctccgagg cacaatacgc atcctctgcc agacggttac gcatctgcgg
    20941 taaagccccc gaccgttaca aagagtcatg agatgccgct acaataccgc cctgaagacc
    21001 gccagcagga agtctgattt gtcgtttttt ccatacggga ggtttgccag catcatgcga
    21061 ccgaaccgca cctttcttct tgcgttgcca cttggagccg ctttctacgg gactgccatc
    21121 gccggagccg ctgacttgcc ccgaaccagg atcaaatggg agaaggtcag cctcgtcgac
    21181 aatctcaacg aagggatcga cgtcgttgac gtcaaccgcg acgggaagct ggatatcatt
    21241 tccgggccgc tgtggtttga ggccccgaac tggacgccgc acccggtccg cgacgtcggc
    21301 atcaacaaca aggagttctt cgagaacaac ggcgaccacg ccatcgatct gaacggcgac
    21361 ggctacccgg acgtcatctc cgcctcatgg ttttcggaca aggtttactg gtatgagaac
    21421 cctggcaagg aaggattggc tgagggccga gcgtggaaac agcacttcat cgttgacgga
    21481 caaaacactt gtgagggcac gctccttcag gatattgacg gcgacaaggt ccccgaactg
    21541 attctcaacc attggaccgc cgagaagccc atgactatca tccgcgtgaa gctgggcagg
    21601 aacggtgctg cgccgcaatt cgagaaggtg gagatcggta agccgcaaac cggtcacggc
    21661 attggcgtcg gggacatcaa cggtgataag cgatccgaca tcgtcctgcc cggcggctgg
    21721 ttcgagcagc cgtcaagcga ctgggccgag aagccatggc cgttccacaa acacaacgga
    21781 ttcaaaatgg atcatagcag cgtgccttgc ctggtcgtgg atgtcaacgg cgacggcagg
    21841 aacgacatca tttacggcaa agcccacgac tacggcttgt tctggatgga gcaggggccg
    21901 gcgaaggacg gagagcccac ttgggcgcag cacgagatcg acaagagctt ctcgcaggtt
    21961 cattgcctgg tctggaccga tctcgacgac gatggccgca aggagatcat caccggaaag
    22021 aggtatcggg ctcacggcga cggcgacccc ggcgcggccg atccgcagtg cctgatgcgc
    22081 ttcatctgga acgacaaaac caagtccttc gataaagacg tcatcagcta cggtgagaag
    22141 atcggttcgg gcatgcagat ccgggtcgcg gacctcgatg gggacaagaa gctggatctg
    22201 gccgtggctg gcaagagcgg aacgtacata ctcttcaatc gcgggccgaa caagtaaccc
    22261 ttgtcaactt gcctcgaagc cgggatgatg ttgtcgtccc tttcagagga gcaagtcatg
    22321 gcaaacgaca agaaggtccg ggttgcgatc attgggctgg ggtttggggc ggagttcatc
    22381 ccgatctacc agaagcatcc gcacgcggag atgtacgcca tctgccagcg gaaccgggag
    22441 aagctcgatc agatcggtga cgccttcggc gttgccaagc gctataccag ctacgaggac
    22501 gtgctgaacg accccgacgt cgacgccgta catatcaaca cgccgattcc cgaccacgct
    22561 ccgatgagca tcgccggcct caaggcgaaa aagcacgtgg cgtgcaccgt tccggccgcg
    22621 acctcggtgt cggactgtcg caagctggtc gaggcccaga aaaaagcccg ccgcaactac
    22681 atgatgatgg agaccgtggt ctacagtcgc gagtttctct tcgtcaagga gctttacgag
    22741 actggtgtcc ttgggcgtat ccagttcctt cgcggttcgc atcagcagga aatggccggc
    22801 tggcccggct attgggaagg cctgccgccg atgcactacg ccacacactg tgtgagcccg
    22861 tgcctgtgcc tggcgggcaa gctggccgag tcggtggtct gccacggctc ggggcggatc
    22921 agcccgaagc tcacgcgaaa gtacggatcg cccttcgccg tcgagaccgc caccattaag
    22981 ctcaagaact cgaacctggt gtgcgaggta acccgtagcc tctttgagac agcccggcag
    23041 taccgcgaga gcttcgacgt gtactgcgac aaggtatcgt tcgaatcacc ccagatcgag
    23101 cacgagccgg ccatcctgca ccgcggcgaa cagcctgagc gggtgacgat cccggactac
    23161 gcccatctgc tgcccgagtc gatccggccg tttaccacca agggcgtcta cgacttggag
    23221 caaaacgtgc acctgtcttt caagcagggc agcggccacg gcggctccca cccgcatctg
    23281 gcccacgagt tcatcacgtc gatcattgag agacgcccgc ccatgcccga cgtcaagacc
    23341 agcgtcaact ggaccatggc cggtatctgc gcccacgaat cggcgatgaa gggtggcgcg
    23401 cgggtgaaga tcccgcagtt ctgagtccaa ccttgacgag ccacggggtt tatccccgtg
    23461 gtctggtgcg atagagccga ccgctgtggg agcgatctcc ggccgcgatc cgctgatccc
    23521 ggattcggat cctcattgtc gtcacatgca caaggggctt catcaccaga gtcacccttc
    23581 tccgatgtac tcgctgatcc tccgaattga ccagcccgcc caaggccggt agcatccgat
    23641 cggttcacgc gtgtcatgga acagggggtt ttccgatgca gagcggcccg gcaatacccg
    23701 aactgatcct gctgcttctg atcggcagcg ccctgatcgt cggggtcatc ttcatcttcc
    23761 gcggcatacg cggtgttccc gtattgagcg atccgaggtg tacgcggtgc gggtacgacc
    23821 tccgatggat caagccggac gtgaacgtcg tctgtccgga atgcgggtcg gacctcaccg
    23881 tcaggcgggc catccgattt gccgagtacc agcgccggcc gcgatcgatc ctggtcgggc
    23941 tggggctgat tgtcgctgtc ttcgtcgtcc ctgtcggctt gcgacttgtg ctgatgcgtt
    24001 cacgtgttgg ccccatgaca ccggcctcgc gtgcctctct cccgagcagc acgctcattg
    24061 ccaacctggc aaccaccgcc agccagccgt gggactggca ggaactcgag cggcggttga
    24121 acgcgggcca actgtcgaaa cgggataccg cggtcgccat cgaccagttg atcaagcaca
    24181 tgcagacgaa acccagcggc tggcgtgagc ccttgcactg gtgtgacaga ttcgtcgctg
    24241 ccgcggacaa agccggcttc atctcagatg aacaacttgt gcgtctgggc caagcatttt
    24301 atggggcaca gccggatgtg aaagcccgca gtcgcctgcg gcaggggcag ccattcacgt
    24361 tcgagcttag ctacggcacc ggctggagtc tgcctggact gaagttggtc atggctttgt
    24421 cacgaactcg acttgaggga gccggagaac tgggggtgtc tgaccccttt cgggacgatc
    24481 gcaagccggc cgaccccgac gatctcagcg ggggcggtcc ctggtccatg attcgtggca
    24541 aagtcgtcag tgatatctcg gcgggaaagc acaatctcgt ctttgaagtc gacacggggc
    24601 tgatcgacga gttcaggcct ttcaagacgg acaatagcat gaagcccggc caggccgaac
    24661 gatggcccaa gacccgttgc aggtggaaca caacggtgac cgttccggtc gaaatcgttg
    24721 cacccgaccg cccggccgtc gatctggtga tcgatgagaa
//
LOCUS       DAOTPW010000059        24674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2109_length_24674_cov_5.055120, whole genome shotgun sequence.
ACCESSION   DAOTPW010000059 DAOTPW010000000
VERSION     DAOTPW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 24674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24674
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_24674_cov_5.055120"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="P5316_07305"
     CDS             complement(<1..867)
                     /locus_tag="P5316_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR84802.1"
                     /translation="MKRLCRMTNSTIAPSRLGFTLIEVLVVVAIIALLIAVLLPSLSK
                     AKEKARQSVCLSNLHQQGIGFAAYAAENRHMLPMVGRFRYTLAEGKYYTLGDPNRSDW
                     IKVNNGLLYRKYVGGNPEVFYCPSNTDADADGDRGMAVFLQRYQHPAASDPQYVDSHN
                     AANSPIGAYTYALPIAAGKSPTDAGAKMYPVEAMIGGPYYSYMTDPTELDHQQALEFL
                     GHFPQYLRGKHSMHALLTDAYFGGYRGYHLNGFNVLYGDYHAKRVPDPAGKIIKGVGG
                     GAVYTPGELATKG"
     gene            1930..5433
                     /locus_tag="P5316_07310"
     CDS             1930..5433
                     /locus_tag="P5316_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84803.1"
                     /translation="MKMQTSSQPRSIRILFWSLAVFVAALILPTAYAAGGSAAVGVAS
                     RPADTQVFTEQSIYIPYEKLREVFEKQGRGVFLPYEEFEKLWRAARAGTKPPPEVGPP
                     VDALITEASHDAVVTKDVVRVTASLKIELLKKGWREVPLHLATSAITGATIGGKPARI
                     IFQPEAGYKLLLDKAEDGSETVDVKLEYVQAYSKAPGQNSVRFMCPQAPVSRWTIRIP
                     EPGIKVNIEPLIAATEVPGGGSGTATQPSETVVLAFVGAASSVRLDWTPKAEGATGLE
                     ALVSVKGEQQVLIEEGVTRSRAALTYEISRAELSQLVIAVPAEQKIVNVLDANVRQWT
                     VKDADGKQHITVQLFEAARASQRVMVELEHFRGEADRSEVGIAVVEAVGVGRQQGIVV
                     VQVASGLRAETTRHTGVLQIDAGELPSALAKTPWSFAYRYGAVPFDLRIQIEKIQPRI
                     TTDSLVEVLLEPDKLSIEVFAVYAIEQAGVFQLEMTVPADFEIRHVAGREAPGIAPVQ
                     VDTHDRQGDDKTRLVMNLSRKAMGRAGLGVRLERVLTEPDLLAPTGKPAALAIPLPRV
                     AGKAIERSIGRMIIHAPEGLRVNPGATEGLRPASFGEAVQGMGTPSEKRFAGARPVLA
                     FIFGQEPAALTLLAERRKPQVTARQLLTASVDSGVVKYEAAFFYDILYSGVKSLRIDL
                     PADLAPDVRNNSPGIRDKVIEPPPKDLREHHVAWSFTGETEFLGAVAIRLTWERKIDK
                     LDLGKSVEVAVPQLRPAEVDRHWGQIVLIKAESIDVQETGEPTGVRPIDPQHDLMPGA
                     SVPNAARAFEFHDDWRLRIQVTRYKLEEVKRTSIERALIQMVVTRGGQTTVQALYRIR
                     SAHQRLAVVLPGGVEFDSEPLRINGRRVPLERGDKDEFFVPLVGLNPEQPFLLELRYR
                     IAGAGRRLSPPAFPSEPAVQKVYLCVFLPPEWTYLGSTGPWTDEMDWTWRPVFDRQPS
                     ASCTNTQLVSWVDEGVSTAAGPVDTFQTDGRMYLFSSLCPAAPPAGALHLITMDEDWL
                     AFLVLAIVLVPGVVLLWTRASVRWTACGVFVVLLIAIGIFFPTLSRQIVDGKLVAAVV
                     IVLTIWCLHYFIRVRPYDPVLIARRQAREQMKLARLGMQAAAAGAGAGVSPAAPGPNA
                     GSPSETNAANKEVREGDSSHA"
     gene            5426..13201
                     /locus_tag="P5316_07315"
     CDS             5426..13201
                     /locus_tag="P5316_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84804.1"
                     /translation="MRKTLLPLLAAVAIPHLSGSAASGIGLTRTGDAMTARELYVPYE
                     DLNVILEGQPRRVLLSREEYAQLKKAAVAEAEARPGHDAAILAADYRVEVQAGRAAIT
                     GELSIEVLVPGIHALALDLAGVGLRKAELDGGNAAIGRSDDGHLDLFVSGKGLHSLRL
                     ELVAPLETTAATQVLNYRLPTPAATRVRLTVPGDVEVKAGAETISREVEQDGSATRFE
                     LVPRQGDVSLVVTLNSKLLRQERVVVARSVIVAEVTQVYERLHATFSMTALHKAVDAF
                     EFRLPAGFEVTYVQSPQMARWSVDSSGGDRILRIHLREQTAGLVVLSLSAIRAAPKLD
                     AWSMPRLIPLDVEGQVSVVGLVLEDRLQPRSLKTDGLIPVDTLVLTRALPASVFQAEP
                     GSPRVRPIIAGYAPQRDFSILAEFHKPEAEMFVTTNLLLTLTDKGHEMRGGFAILPKE
                     EKRFSLDFAVPEGWDVTTVTDAENKTLPIERFDRPKGGTRVHVRLPQGVAPLQEYRIY
                     FQASHQPAGWLALWTTRETAFPIFGVLGASRESGALAVAVKDDIAVRPSETERIEPLD
                     EHEKEKYGLGGVPTQLAFRFEGADYRMTLAVERVKPRLTARSISSFVVTPDFLIAHYE
                     VFYEIQEAATRELALLLPKSMPESLVIRGLGPARLKEYSSQLDGDYRRWQILLAEPGR
                     GSVRLAVDCRQPLTEEGTSTLSLPVVKAEGVSYQSGLVSVEGSAELNVEVKTDLGKAD
                     VGELVDAEYQPGKRLLGVYRFVGDQPAITVNVSRDVAYMLPAVIVQRAELATLLDAGG
                     LAQTAARYALRTKAPFVQLELPSGSKLWSVEVDGLPAAPQREGESLMISLPAKAGGTL
                     RDLRVVYETQIEGVALFGNLEVSAPRLRLRGEEEATAIDVPAADLLWRLYTPSGYEVL
                     STAGTVVTEQVTPSSLALSNVAQWLFKTGGGVNWFYGSSGLARARAMAKVGMRLKYGE
                     LNAVSQEEDKRPVWGVSMQPREAAIEPRQPAPPPPPPAVQTAGSPGQPRPSVALAPQA
                     ASTTKPPLKPQQAGIGWALSGVRSLRIELQRGGKEIVFHSLGEEPKLVVGVAHTRRSS
                     SLAWALAGLVILVGLARVNWSVKSRATMVVAIIGVATILPLIAGSAALAQVLNPACYA
                     GFALIPLYVAISVVRRLAVRFRPNSAVSNLVATAAATLLVMLVLCPAAHAETSNDHMI
                     RSLVERLQPPAPAGVPADAVIVPYDPKTGTGIRDAAQLLVPYDQFVELWNQAHPDRQI
                     EAIKPPKSYGLAGATYMATLQGDEYLLVEGVMEIEVYTDEMVSIPLGLEGGVLAGATL
                     DGRTARLSIATVSADQPSQAVQVPAKGKRPPRGSVFLLHVTGKGRHRLELSVRMRLDR
                     PGGWRVAQGQLPAAPATALSLVIPQAQTELRIGQVADRRTLVTSGPGEVLNTALNADG
                     KFDVSWRPKVGEAAIDRSLTVESSARFDVQEDGLMLDWTLRLSFRRGEREFFRFGLPT
                     DYIVLQVTGSNVRGWETRDVDGRREIEVTLLKPAKDRDEIAVHLWHRGKVGDESFSTF
                     ELPMVSVPDAILHQGSVAIRRSPLLNLRTIEVSGVARTDLTDTSASRPADNTSPLGIR
                     PYQAYRFAATPFVIRLEATPVSARVTARFQTILRVAERERMLESRVILKVEDRPLYQV
                     RIALPADLKVDRVTAPGVFEWAIAQQAGGPLLNIYLAGGQRGDVPITLTGTLGKPGPV
                     EQVPLPRLAVQDVAEQEGDIVVQGDPAYRIEAEDLVDCESVLLTRVFGWLTDAQRAPA
                     QLAVHYRGDKYKGSVRVTQRQPDVSCHTVSSVRVTSRAIEETVLLDFNVRDAGIRELS
                     FLLPAQMKDARISVPQLRLKTIQPTSDGTGAPIRVRLELQDRLMGQVRVLIENDRLLT
                     AAVQSVPIPVVETGRTVRRYVVLESAGRSEVVIESSPGLDALTREQQEWRWLSSVLGG
                     KVTQAYLVQPEAQQPALKFRTQERAVVETAGASIGLAETLMVVDAQGTYRAGQILRVN
                     NATEQYLEILMPDGAVLWTARVAGEPVKPTQVPDRTKARHVRIPLIKTATGDLDYEVV
                     LKYGGRLGPIGITGSVTFPVIETVNIEVALSQVRLYLPETHRWFRFGGTMRQAGDEVE
                     LAADYVTYQTRKARQLAEALSSADDYAKVRAAENVRVLGGQMQEFQSFNSAIVQEGRL
                     RKELLSNAMVISQAGEQVKELAKVLEQGAVTDNRGDLNDYFAGQANTRSSNVVMELGT
                     NFETGDASAATLQPGGDDGFSHDWFVANGLVSPAPQSIIWDAPATAPAEVQDLAGIQG
                     VANLYFSVRQEGAKARQPAVPQVIQEAQMEDLRQSLDRVQVSARERKKEDVLDGKGDT
                     LTRYQDVVRERASVSQRRSSMSYGHGVELGHRSTITLDAMDDADGDDAKDRSRYRGTM
                     GGMGMGGMGGGMEGMGAGYGAYAGGGDVMTGGAGAGVLTARVPGGFSAKGLASLDVDI
                     PVRGQVYFFTTPQGKAEITARAVSRGFLSNLRYLVVVLVLAGAALACYRALAQKAFTC
                     CTSRRGAVVTIVLGLIAVIVGFLPVVGLLAVAVGLIVLIRARGKVAAGPLQAT"
     gene            complement(13258..14634)
                     /locus_tag="P5316_07320"
     CDS             complement(13258..14634)
                     /locus_tag="P5316_07320"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRR84805.1"
                     /translation="MAETTAPTLSGLSVPQQQGRLVSLDVFRGITIAGMILVNNPGSG
                     SHVYAPLRHADWNGWTPTDLVFPFFVFIVGVAMTFSFDRRIAQGAGRPALFGQVVRRT
                     IILFVLGLILNGYLTCFPAWDALAKPSGWKIMGPFVAVIVGLALMFADRGPFCWPRGT
                     WPQMRMVAGWLLTIGAIATFATCFDAFKESRIRVPGVLQRIALCYFFSSIIVMYSGVR
                     GRAIWTAVLVVGYWALLRYASPPGDFRAELLPPDRPEGLLHEWVDLRMFRLGNHLYGE
                     RPDPEGVLSTIPAIATCLLGVLAGNWLHSSRDKRDTAAGLFLAASAAIAVGYFMNYSV
                     PINKKIWTSSYVLFTGGLALQSLAVSFWLVDVKRYRRWAWPFLVFGTNAIAVYFVSGI
                     FGRLMMTYKWHLADGSEISLRTWLYQTIFASWAGPFNASLLWAITYVLLWLLLTAPLY
                     RRRIFIKV"
     gene            complement(14774..15676)
                     /locus_tag="P5316_07325"
     CDS             complement(14774..15676)
                     /locus_tag="P5316_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009369183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRR84806.1"
                     /translation="MSTALATIDLWSSVILPAIDEALRRILPTKWGEYQFMRYALLEC
                     LLLAPVCAAVGVKVVNFRMAFFSDAISHSAFAGVAIGLLLHESFSAADPRMTLVLFGL
                     IVGLGIAYVRRTTDLSNDTVIGVFFSAVVALGIAIVTRSGARTTQFQRYLYGDILNLD
                     SADLGLSLLLGIAVLVFMIAGFNRLILVGLNDELAHSRGIRVRLYDYLFTLLLALVVT
                     VSIRTAGILLVTALLVIPAATARNLARSAGGMFWWAIAFGLLSGVAGTLASFSDYLAN
                     VGTGAVIVLASVFFFIISALLRHK"
     gene            complement(15673..16491)
                     /locus_tag="P5316_07330"
     CDS             complement(15673..16491)
                     /locus_tag="P5316_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRR84807.1"
                     /translation="MTLNAVPDNAAPTFALRLTNVGVTLGARPILEHITFEPAVGQIN
                     AIIGPNGAGKTTLLRAILGLVPYTGRIEWGASRNGKPLRVGYVPQRLDFDRGIPMTVM
                     DFMCAGLQRTPVWLGHHRQARQLAQTWLDRVGGKDWADQPLGRLSGGELQRVLLAMAL
                     SDEPEILLLDEPVSGVDVTGEELFCDLLASLQRDSRYTMLLVSHDLSIVTRHAQYVLC
                     LNKTVKCSGRTVETLTAENLKALYQQEVALYAHTGEHEGHSHGHTHHHGGSCGK"
     gene            complement(16488..17573)
                     /locus_tag="P5316_07335"
     CDS             complement(16488..17573)
                     /locus_tag="P5316_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRR84808.1"
                     /translation="MKPASGAHWRTILLIAVILAIVVAVVFLPSGRDSQQAARLKVVC
                     TFLPNYVFTRNVVGDTPGVDIRLLVSTAVGCPHNYVVTPADLKAVSQADLIVANGLGL
                     ESFLDTLQKANPKARLITISDDCEILQSRTSHHHKHHSAETAASRPATDRQAAPNRNE
                     HEHDNHHQGAATAPAPETDDHYDEEHAAGEPNPHVWVSPIQAIRQIRNLAHRLSEADP
                     QYAARYQANAEAYVARIQALHRRLREASVRFANRRIVTFHDAFAYLAKDLDLEVVATL
                     TLDPSRPPSAAEMTEVIDAIRRTGAAAVFFEPAYSDAVARTISRDAGVPAFPLNPFNS
                     LDVDPTERSYEEVMEANLEVLVKALGS"
     gene            17809..18228
                     /locus_tag="P5316_07340"
     CDS             17809..18228
                     /locus_tag="P5316_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84809.1"
                     /translation="MKVVVLFTVGADETLIVGLAPLSLGYLCRVGEMAAGAHDGLEVG
                     RMGLEPVGEELRKHAPAPAKRADGMKPLKNGVADKRGLVGDIVQDLGQMLIDTEGNDG
                     LFGLVWHKSNSLPAIGVAGVIFRYHSRSARIVPGGQP"
     gene            18262..18525
                     /locus_tag="P5316_07345"
     CDS             18262..18525
                     /locus_tag="P5316_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84810.1"
                     /translation="MPKEPDLFEMYRWVLMIICGVYTLACLGQTLVGWLDYFSQSRRT
                     QVLGHYAVVLLLRARLRRFARDLWQIGVLLAIFVGLVYAHRGF"
     gene            18640..19494
                     /locus_tag="P5316_07350"
     CDS             18640..19494
                     /locus_tag="P5316_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84811.1"
                     /translation="MSEQQGKSAAIDDLPVDELVAYGRTLGMHLNANAGRGELLRLVR
                     ERQALLAELEHEVLLDIVVWARRPVRQSASKEELAKEIASVKRVDFQGLSDRGLVALA
                     RLRGVPVSPKQPRKELEKAVRASEPFWDKITRKRRRLIGSLISHLLDDAEPESKEAYR
                     FLPEESGHTSLKERIAEQGVVSGLARTIRGVADDYVRQKMDEIEARIDRKLDEIDRRL
                     AEWRDREIANRLKIIKITLVASILVALMSYGYTLLKSNIKPVPAGVEGIPVSAPAGGA
                     LTRPAEAD"
     gene            19500..19952
                     /locus_tag="P5316_07355"
     CDS             19500..19952
                     /locus_tag="P5316_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRR84812.1"
                     /translation="MYERLDKGLERVIKVAHEIAREYEQEYVGTEHLLLAIAREREGL
                     SGQVLAEVGASEEKTKAAVDRLIKNSLEDTWVFGRLPGTPHFRNVMAKAIQEARNFGS
                     KEVSTEHLLIGLLLEKGSVGYEALKSLGLTPKLIRDKIRRLMAEGAVD"
     gene            20231..20581
                     /locus_tag="P5316_07360"
     CDS             20231..20581
                     /locus_tag="P5316_07360"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRR84813.1"
                     /translation="MSRLAELKCEACRRDEPCVTRAESLDLLKAIPDWRIIECDGLQR
                     LERVYTFSNFARAVDFTDQVASLAEEVGHHPAILTEWGRVTVHWWTHKIKGLHRNDFI
                     MAARTDEVYAGHVR"
     gene            complement(20585..21034)
                     /locus_tag="P5316_07365"
     CDS             complement(20585..21034)
                     /locus_tag="P5316_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HRR84814.1"
                     /translation="MIVDSLKNADLYKTIHPLLPKAIDFLRRPDLATLPAGRYPIDGD
                     RLFAFVCDNQTKPANQGKWEAHHKYWDVQYVVRGRERMGYARMNGASIREPYNPETDL
                     VFFNTRGDFFDVEEGMFAVFTLQDIHMPGLAVEKPEPVRKVIVKVAP"
     gene            complement(21085..22689)
                     /locus_tag="P5316_07370"
     CDS             complement(21085..22689)
                     /locus_tag="P5316_07370"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HRR84815.1"
                     /translation="MRTRIDHATIITCRGNDPVVLSDHSVEFRDGVITGVGPTDHVTE
                     TNGRPTNQPPPELKGRPLGTILLATGKVTQEQIQEALQIQGQSHGVIGQELLHLGYVS
                     EADIEYALSIQSGGISQAGTARANDLTVIDGRDCLVIPGLINTHHHLFQSLTRCAPHV
                     QNACLFDWLVGLYPRWQHLTYDALHLAALITIAELILNGCTTTSDHMYLFPPASDVAA
                     EAVLRAAETLGIRIHFCRGSMTLGRSAGGLPPDECVETDEHVLADSVRVIELYHDPSP
                     LAMRRIDLAPCAPFNITPALLDQTRDLAAERDVLLHTHIAETLDEERYCLDRFGVRPL
                     EYLRQHRWLGPNVYLAHCVHVDEAEIRLLAETSTGVAHCPCSNMRLGSGIAPVCKMLD
                     AGIKVGLAVDGSSSNDAGNLLAEARQALLLQRVVNGAGAMSPADAFRLATAGGAAVLG
                     RPKLGRIEPACAADLVLYDATDIAMAGGAAQDALGALLLCQPSRPKRVIVAGRTLAQD
                     GRLVGIDQAGLAADFNQLVRQQFTNG"
     gene            complement(22773..22982)
                     /locus_tag="P5316_07375"
     CDS             complement(22773..22982)
                     /locus_tag="P5316_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84816.1"
                     /translation="MLVRHPDQVVVAERHLLVGLARIESSHPMATPRSADAIARDWMI
                     INLLHISMIEPQNGNGRRRRSGRST"
     gene            23519..24613
                     /locus_tag="P5316_07380"
     CDS             23519..24613
                     /locus_tag="P5316_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR84817.1"
                     /translation="MAQVFCIAAKSSPPLPTRTYPQTVWVGFQVRPARCRSRTGAAGF
                     RGDRLARAWAWRRGFTLLEVLIVVAVVALLAAILVPALSGARDQARSLACRSNMKQLM
                     NGVSFYVADYHVLPGTHSLFFFQALFGAAWPRPSGVTWDGARDRLVGLTFKAAYAEPH
                     HLDPEFIADVPRKGTIFRYTKEERIYTCPSDRPGKAEDTALGGGGNGRLGYSLNAYIG
                     YKAPEHLQSFTYVAASPDNRLPGGQKRRSFAAGQRVVFSPGAFMVLFEEHPFYHMNTS
                     FPEGNFNCLDRIATRHLLKGGAKGGTPEGRTSVVFLDGHAESRLYPAKTMGRELFAEF
                     GQPYVWRESGPPDTTNMTAFITRLRGPCPW"
BASE COUNT         4766 a         6870 c         8286 g         4752 t
ORIGIN      
        1 gcccttggtg gcgagttctc cgggcgtata gacggccccg ccgccgacgc ctttgatgat
       61 cttacccgcc gggtcgggaa ctctcttggc gtggtagtcg ccatagagga cgttgaaccc
      121 attgaggtga tatcccctgt acccgccgaa ataggcatcg gtgagcagcg cgtgcatgct
      181 gtgcttacct cgaagatact ggggaaagtg tccgaggaac tcgagagcct gctggtggtc
      241 gagctcggtg ggatccgtca tgtaggagta atagggcccg ccgatcatcg cctcgaccgg
      301 gtacatcttg gctccggcgt ccgtgggaga ctttcctgcg gcgatgggca aagcataggt
      361 gtaggcaccg atgggagaat tggctgcgtt gtgcgagtca acgtactggg gatcggaggc
      421 agccgggtgc tggtatcttt gcaggaagac agccatgccg cggtcgccat ccgcatcggc
      481 atcagtgttg ctgggacaat agaatacttc gggattgccg ccgacgtatt tgcgataaag
      541 gaggccgttg ttgaccttga tccagtcact tcggttgggg tctcccaacg tgtaatactt
      601 gccttcggcg agcgtgtacc taaaacgccc caccatgggg agcatgtggc gattctcggc
      661 ggcataagcc gcaaagccga ttccctgctg gtggagattg gacaaacaca ccgactgcct
      721 ggccttttcc ttcgccttgc tgagagaagg gagcagtacg gcgatcagaa gggcgatgat
      781 ggccacaacg actaggacct caataagggt aaagccgagc cgagaaggtg caatcgtact
      841 attggtcatc cgacaaagac gcttcatgat gggggccctc acctcgctgt tcttgcgttg
      901 ccagccgttc caaaccgaca ggcaccgacg gaacactcgc caaccacaag tattttaacg
      961 tgtcaacgga gccaagtcaa ccttgtcgac aaaatatccg gggggcattt catccggtag
     1021 acctccaacg aggtcggcca gcctatatat gcgatctccg gtggggcagc tcgcagcctg
     1081 gcgatgagca cgttgggggc ctttagacag caaaaataga aataaataag tgagaatcgt
     1141 attccctcgt gactcagatt tcggactgtg cctggcgcag gaaccttggg tgtgcagctt
     1201 gagccgtttc ggcgtccggg gtctgcaaca cgtgcggaag cggctgcata cccgggcact
     1261 gtcacggacc ctctgtcagg ggggtggcag acgggtttcc agtgcccccc gcctgaaccc
     1321 gcaggaacct ttcgggtggt ttgaggcgcc cagtggtacc caggcgtcat gcgcggctct
     1381 tgcgggcggg aatgcacgtt tcgagggttg gttggctgcg gcgcttgtgg agacctcaga
     1441 aatccaaggc ggccttcagc cgtaaccaga acgcgcgcgc agaatgcgca ccagtcgcga
     1501 cggaagagac taaggcggac cgcctttttg cgggccgcaa cccaagcgga gcgcgatcca
     1561 aaaagatgcg tgcaaacccc accgctttct ggccgtaagg ctttagccag gtaagcgaag
     1621 agtcatgggt gaaggagcgc cttcggtgcc gccggtgaaa aaagttacat ggccgttaca
     1681 ggaaaacgaa accacgggtg ggcgctttcg tctaaactgc tgtacgggca ggacgctgcc
     1741 agggcggcta agccggcggt ttagtggcag agcagtctac ctgcgtaccg acgtagggac
     1801 ctgccgatca tggcgaccct gacgtcatgt catcgtgacg agttaagcaa cccccggtgc
     1861 attggggtgt aggaatgaat gacgcttctc ctgtcgtgta gattgtcagg ccggtcaaga
     1921 ttcgagggaa tgaaaatgca aaccagttcc cagccgcgat cgatccgtat ccttttttgg
     1981 agcctcgccg tgtttgttgc cgctcttatc ctgccgactg cctatgcggc aggtgggtcg
     2041 gcggctgtcg gagttgccag ccgtcctgca gacacgcagg tcttcaccga gcagagcatc
     2101 tatatcccgt atgagaaact gcgggaggtt ttcgagaagc aagggcgagg tgtgtttctt
     2161 ccctacgagg agttcgagaa gctttggcgg gccgcccgcg caggcacaaa accaccccca
     2221 gaggtgggac cgcccgtgga tgcgttgatc acagaggctt cgcatgacgc ggtggtgaca
     2281 aaggatgttg taagagtgac ggccagcctc aagattgagt tgctgaagaa agggtggagg
     2341 gaggtgccgc tgcatcttgc cacatcggcg ataacaggag ccacgatcgg cggcaagccg
     2401 gcgcggatca tcttccagcc ggaggcaggg tacaagctgt tgctcgacaa ggcggaggat
     2461 ggttcagaga cggtcgacgt caagctcgaa tacgttcaag cctacagcaa ggcgcccggc
     2521 cagaacagcg ttcgtttcat gtgcccgcag gcgccggtca gcagatggac gatccgcatc
     2581 ccggagccgg gcatcaaggt gaatatcgag ccgctgatcg ctgcgacgga agtacccggc
     2641 gggggttccg gtacggcgac gcagccgagt gagacggtcg tcctcgcgtt tgtgggggcg
     2701 gcgtcgtccg ttcggctgga ttggacgccg aaggccgagg gggcaacggg cctggaggcc
     2761 ctggtcagtg taaaaggaga gcagcaggta cttatcgagg agggcgtcac ccggtcccgg
     2821 gcggcgctga cctatgagat cagtcgtgcc gaactcagcc aactggtaat tgcggttccg
     2881 gccgagcaga agattgtcaa cgttctggat gcgaacgtgc gtcaatggac ggtcaaggat
     2941 gccgatggca aacagcacat caccgttcag ctttttgagg cggcgcgagc ttcccagcgt
     3001 gtcatggttg agctcgaaca tttccggggc gaggccgatc gcagcgaggt gggcatagca
     3061 gtggttgagg ccgtcggcgt ggggcgtcaa cagggcattg tagtggtcca agtcgcttcc
     3121 ggacttcgag cggagacgac acgacatacg ggcgtgctcc aaatcgatgc cggggaactg
     3181 ccgtctgcgc ttgcgaagac gccgtggagt tttgcgtacc ggtatggggc ggtgcctttc
     3241 gacctgcgta ttcagatcga gaagattcag cctcggatca cgactgactc gcttgtcgag
     3301 gttctgctgg agccggacaa gctgagcatc gaggtattcg ccgtgtacgc catcgagcag
     3361 gcgggtgttt tccagcttga gatgacggtt ccggccgatt ttgagatcag gcacgttgcc
     3421 ggccgtgagg ctcccgggat tgctccggtg caggtcgaca cgcatgatcg gcagggagac
     3481 gacaagacca ggctggtgat gaacctctcg cgtaaggcga tggggcgtgc gggtctgggt
     3541 gttcggttgg agagggtgct gacggagccc gatctgcttg caccgacggg caagcccgcg
     3601 gccctggcga taccgctgcc tcgcgtggcc ggcaaagcca ttgagcgaag catcgggcga
     3661 atgatcattc acgcgccgga gggacttcgg gtcaatccgg gagccacgga aggcctgcgg
     3721 cccgcgtctt ttggtgaggc cgtgcagggc atgggaacgc ccagcgagaa gcggtttgcc
     3781 ggggcgaggc ccgttctggc gttcatcttc gggcaggagc ccgccgcgct gacgctgctg
     3841 gccgagcggc gtaagcctca ggtgactgct cgccaactgc tgacggcgtc ggtggattcg
     3901 ggagtggtca agtacgaagc cgctttcttc tacgacatct tatacagcgg ggtcaaatcg
     3961 ctgcggatcg atcttccggc cgatctggcc ccggacgtac ggaacaactc gccggggata
     4021 cgcgacaagg tgatcgaacc gccgccgaag gaccttcgcg agcaccacgt cgcctggagt
     4081 ttcacggggg agaccgaatt ccttggcgcg gtcgcgattc gtctgacgtg ggagaggaag
     4141 atcgacaagc ttgacctcgg caagagcgtg gaagtggcgg tccctcagct cagaccggca
     4201 gaggttgacc ggcattgggg tcagatcgtc ctgatcaagg ccgagagcat tgacgtccag
     4261 gaaaccggcg agcccacggg cgtccgaccc atcgacccgc agcacgacct gatgcctggg
     4321 gcgagcgttc cgaacgcggc aagggctttc gagtttcatg atgactggcg gcttcgcatc
     4381 caagtgacgc gctacaagtt ggaagaagtc aaacgcacga gtatcgagcg tgcattgata
     4441 cagatggtcg tgaccagagg cggtcagacg acggtgcagg cgctctatag aatccgcagc
     4501 gcgcaccagc ggctcgcggt ggtcctgccc ggcggggtgg agttcgacag cgagccgctt
     4561 cggatcaacg gtcgtcgggt tccgttggag cggggcgaca aggatgagtt ttttgtgccg
     4621 ctcgtcggcc tcaatccgga gcagccgttc ctgcttgaac tgcgataccg gattgcgggg
     4681 gccggtcggc ggctgtctcc gccggccttc ccgtcggaac cggcagtgca gaaggtatat
     4741 ctgtgcgtat ttctccctcc ggaatggacg tacctgggtt caaccggtcc gtggacggat
     4801 gaaatggact ggacatggcg gccggtgttt gatcggcaac cgtcggcgag ttgcacgaat
     4861 acgcagttgg tttcctgggt tgacgaaggg gtttccactg cggcgggccc ggtggatacg
     4921 ttccagaccg acggacgcat gtacctgttc tcatcccttt gtccggcggc gccgccggcg
     4981 ggagcgctgc atctcatcac gatggacgaa gactggctcg cattcctggt gttggcgatc
     5041 gtcctggtgc ccggcgtggt gctgctatgg acaagggcgt cggttcggtg gacggcctgc
     5101 ggcgtctttg tggtcttgct gatcgcaata gggatcttct tcccgacgct gagtcgacaa
     5161 atcgttgacg gaaaacttgt ggcggcggtc gtgatcgtgc tgaccatctg gtgcctgcat
     5221 tacttcattc gtgttcgacc atatgatccg gtcctgattg cccgtcgcca ggcccgcgag
     5281 cagatgaagt tggcccggct ggggatgcaa gcggcggcgg caggagcagg agcaggagtc
     5341 tcaccggctg ctccggggcc aaacgcgggc tcgccgagcg agacaaatgc tgcgaacaaa
     5401 gaggtgaggg aaggagactc aagccatgcg taagacactc cttccactcc tggccgcggt
     5461 cgctataccg cacttgagcg gctccgcggc aagcgggatc ggcttgacgc ggaccggcga
     5521 tgcaatgacc gctcgcgagc tgtacgttcc atatgaagat ctcaatgtta ttcttgaagg
     5581 tcagccccgg cgcgtgctcc tgtcgcgaga ggaatatgcg cagttgaaga aggcggccgt
     5641 cgccgaggcg gaggctcggc ccgggcacga cgcggccatt cttgcggccg attatcgcgt
     5701 ggaagtccaa gccgggcgtg cggcgatcac aggcgagctt tcgatcgagg tgctcgtgcc
     5761 gggcattcat gccttggccc tggatctggc gggggtgggg ctgcgcaagg cggaacttga
     5821 tggcggcaac gccgcgatcg gtcgctcgga tgacggtcat ctcgacttgt tcgtgagcgg
     5881 caaaggcctc catagcctgc ggcttgagct tgttgcgccg ctggagacga cggcggcgac
     5941 gcaggtgctc aactaccgtt tgccgacgcc ggcggccacg agagtgcgac tgaccgttcc
     6001 gggtgacgtg gaggtcaagg cgggtgcgga aaccatcagc cgcgaggttg agcaggacgg
     6061 cagcgccacc cgtttcgaac tcgttccccg gcagggggac gtgtcgctgg tcgtgacact
     6121 gaacagcaag ctgcttcggc aagaacgcgt ggttgtcgct cgcagtgtca tagtggctga
     6181 agtgacgcaa gtctatgagc ggttgcacgc gaccttctcc atgacggctc tccacaaggc
     6241 cgttgatgcg ttcgaattcc ggttgccggc gggtttcgag gtgacttatg tccagtcgcc
     6301 gcagatggcg cggtggagcg ttgattcgag cggcggtgat cgcattcttc gtattcattt
     6361 gcgcgagcag acggccgggc ttgtggtctt gagcctttcg gcgattcggg ccgcccccaa
     6421 gcttgacgca tggtccatgc ccaggttgat tccgctcgac gttgagggac aggtatctgt
     6481 tgtcggcctg gtgcttgagg atcgtctgca accccggtcc ctgaagacgg acggcctgat
     6541 tcccgtggat acgcttgtgt tgacacgggc tttgccggcc tcggtgtttc aggcggagcc
     6601 gggatcgcct cgcgtgcgac ccattattgc ggggtatgct ccgcagcgcg atttcagcat
     6661 cttggccgag tttcacaaac ccgaggccga gatgttcgtc accacgaatc ttctgctgac
     6721 cctgacggac aaggggcacg agatgcgcgg cggctttgcc attctgccca aagaggagaa
     6781 gcgcttcagc ttggatttcg cggtcccgga agggtgggat gtcaccaccg tcacggatgc
     6841 cgagaacaag acactgccga tcgagcgttt tgaccgcccg aaaggcggta cacgagttca
     6901 cgtcaggctc ccgcaggggg tggcaccgct gcaggagtat cgaatctatt ttcaggcctc
     6961 acaccagccg gccgggtggc tggccctctg gacgacaaga gaaacggcgt ttccgatctt
     7021 cggtgttctg ggagcctctc gtgaaagtgg cgcgctggcc gtggcggtta aggatgacat
     7081 tgccgtgagg ccgagcgaga ccgaacggat tgaaccgctc gacgagcatg aaaaggagaa
     7141 atacggcctg gggggcgtac cgacccagtt ggctttccgt tttgagggag ctgattaccg
     7201 gatgacactg gccgtcgagc gggtgaagcc cagactgacg gcgcgaagca tatcatcatt
     7261 tgttgtcaca cctgactttc tgatcgctca ctatgaagtc ttctatgaaa tccaggaggc
     7321 cgccacccgc gagctggccc tgctgctgcc gaagagcatg cctgaatcgt tggtcattcg
     7381 tggactcggt cctgcccgcc tgaaggaata ctcaagccag ttggacggcg attacaggcg
     7441 ctggcagatt ttgttggccg agcccggccg agggagcgtt cgtctggccg tggattgccg
     7501 gcagcctttg accgaagagg gcacaagcac gctgtctctg ccggtggtga aggccgaagg
     7561 agtgtcatat cagtcgggcc tcgtatccgt ggaaggaagc gcggaattga acgtcgaggt
     7621 caagacggac cttggcaagg ccgacgtggg tgaactcgtt gacgctgagt atcagcccgg
     7681 gaagcggttg ctcggcgttt atcgttttgt tggagatcaa cccgcgatca ccgtgaacgt
     7741 gtcgcgggat gtcgcttata tgctgccggc ggtcattgtc cagcgagccg agctggcgac
     7801 gctgcttgac gcgggtggcc tggcccagac ggcggcccgc tatgcgctgc gtaccaaggc
     7861 gccgttcgtg cagcttgaac tgccttccgg ttcaaagctg tggtcggttg aagtcgacgg
     7921 tctgccagcc gcgccgcaac gagagggcga gtcgttgatg atcagtctgc cggccaaggc
     7981 gggcggcaca ttgcgcgatt tgcgcgtcgt ttatgaaaca cagatcgagg gcgtagcatt
     8041 attcggcaat ctcgaggtgt cagctcccag gcttcgtctg cgaggtgaag aagaggcgac
     8101 tgcgattgac gttcccgctg ccgacctcct gtggcgattg tacacgccca gcggctatga
     8161 ggttctctca acagccggaa ccgtcgtcac cgagcaggta acgccatcga gtctcgcttt
     8221 aagcaacgtc gcccagtggt tgttcaagac cggcggtggt gtgaactggt tctacggatc
     8281 gtctggactg gcccgtgctc gtgcaatggc gaaagtcggc atgaggctga aatatggcga
     8341 acttaatgcc gtttcgcagg aggaggacaa acgccctgtt tggggtgttt ctatgcaacc
     8401 acgcgaagct gccattgaac cgcgacagcc tgcgccgcca cccccgccac cggctgtcca
     8461 gaccgcaggc agccctggtc agccacggcc atcggtggcg ttggcgccgc aagcggcatc
     8521 gaccacaaag ccgccgctca agccacagca ggccggcatt ggctgggcgc tgtcgggcgt
     8581 tcgcagtttg agaattgagc tgcagcgagg cggcaaagag attgtgttcc acagtcttgg
     8641 cgaggagccg aagcttgtgg tgggtgtcgc ccatacgcgg cgatcaagct ccctggcgtg
     8701 ggcactggcg ggccttgtga ttctggtcgg tctggcaaga gtgaattggt cggtgaagtc
     8761 tcgcgcaacc atggtggttg cgatcatcgg ggttgccacg attcttccgc tgattgccgg
     8821 atcggcggct ctggcccagg tcctgaaccc ggcctgttac gccgggttcg cactgattcc
     8881 gctctatgtc gccatctcgg tggttcgccg actggccgtc cggtttcgtc ccaactccgc
     8941 agtctcgaac ttggttgcca cggccgcagc cacgctcttg gtcatgctgg tcttgtgtcc
     9001 ggcggcccat gctgaaacga gcaatgacca catgattcgc tcgctggtcg agcggctgca
     9061 accgccggcg ccggccggcg tgccggccga tgcggttatc gtgccctatg accccaagac
     9121 gggtacgggt atccgggatg ccgcccagtt gcttgtgccg tacgaccagt tcgtcgagtt
     9181 gtggaaccag gctcacccgg atcggcagat cgaggcgatc aagccgccga aatcttacgg
     9241 tcttgcgggg gcgacctaca tggcgacgct gcagggcgat gagtacttgc ttgtcgaagg
     9301 ggtcatggag atcgaggtat acaccgacga gatggtttcg attccgctgg ggctcgaagg
     9361 gggggtgctt gccggcgcaa cgctggacgg caggacggcc cggctgagca tcgccacggt
     9421 gagcgcagat cagccctcgc aagcggttca agtccccgcg aaaggcaagc gcccgccgag
     9481 ggggagcgtc tttttactgc atgtcaccgg gaaagggcgt caccgtctcg aattgagtgt
     9541 acggatgcgc cttgatcgtc ccggcggctg gcgggtagcc caagggcaac tgcctgcggc
     9601 tcctgcgacg gcgctcagcc tcgtcatccc acaggcacaa accgaactcc gcattggcca
     9661 ggttgcggac cggcggacgc tcgtcaccag cggtcccggc gaggtcctta ataccgcact
     9721 caatgctgat ggtaagtttg acgtgagctg gcggcccaag gtcggggaag cggccatcga
     9781 tcggagcctg acagttgaat cctcggcgcg attcgacgtc caagaggacg gtttgatgct
     9841 ggattggacg ttgcggttgt catttcgccg cggcgagcgg gagtttttcc gtttcgggtt
     9901 gccgaccgac tatatcgtgc ttcaggtgac cggcagcaac gtgcgcggct gggaaactcg
     9961 cgatgtcgac ggccgccggg agatcgaagt gaccctgctg aagccggcca aggatcgcga
    10021 tgaaatcgct gttcacctct ggcatcgggg gaaagtggga gacgagtcgt tttcgacctt
    10081 tgaattgccc atggtgagcg tgccggatgc gattcttcac cagggttcgg tggccattcg
    10141 ccggagcccg ctgttgaatc tgaggaccat cgaggtatcc ggcgtggctc gcaccgatct
    10201 gacggacacc tcagcctcgc gaccggccga caatacaagc ccgctgggga tccggccgta
    10261 ccaggcctat cgattcgcgg caacaccgtt tgtgattcgc ctggaggcca cgcctgtaag
    10321 tgctcgtgtc acggctcggt ttcaaaccat ccttcgggtt gcggaacgag agcggatgct
    10381 cgagtcacga gtaattctca aggtggaaga ccgaccgctt taccaggttc gcatcgcctt
    10441 gccggcggat ctgaaggtcg atcgggtcac ggccccgggg gtcttcgaat gggcgatcgc
    10501 gcagcaagcc ggcggaccct tgttgaacat ctacctggcc ggcgggcagc gtggggacgt
    10561 tccgatcacg ctcaccggta cgctcggcaa gccgggtccg gttgagcagg tacccttgcc
    10621 tagactggcg gttcaggacg ttgccgagca ggagggcgac attgtcgttc agggcgatcc
    10681 ggcgtaccgc atcgaggcgg aggatctggt tgattgcgag tcggtcctgc tcacgcgcgt
    10741 tttcggctgg ctgacggatg ctcaacgtgc gccggctcag cttgcggttc actaccgtgg
    10801 ggacaagtac aaaggctcgg tgcgcgtgac acaaaggcag ccggacgttt catgccacac
    10861 agtcagcagc gttcgggtga ccagccgggc gatcgaggag acggtccttc tggacttcaa
    10921 cgttcgcgat gccgggatca gggaactcag ttttctcctg ccggcacaga tgaaggatgc
    10981 ccgtatcagc gtgccccaac tgcgtctgaa gaccattcag ccgacctccg acggaacggg
    11041 tgcgccgata cgcgtgcgtc tggagttgca ggacaggctc atggggcagg tgcgggtgct
    11101 gatcgagaac gatcggcttc tgacggcagc ggttcagagc gtgccgattc cggtggtcga
    11161 gacgggacgc acggttcgcc gatatgtcgt gcttgaaagt gccgggcgaa gcgaggtcgt
    11221 catcgagtcc tcgccggggc tcgacgccct gacgcgcgag caacaggagt ggagatggct
    11281 cagttcggtt cttggcggga aggtgacgca ggcttatctg gtacagcccg aagcccagca
    11341 gcccgcgctg aaattccgca cgcaggagcg ggccgtcgtc gaaacggccg gggcatcgat
    11401 cggcctggcc gagacgttaa tggtcgtcga cgcacagggc acatatcggg cgggccagat
    11461 cctcagggtg aacaacgcca cggagcagta tctggagatt ctcatgccgg atggggcggt
    11521 gctctggacc gcgcgagtcg cgggcgagcc ggtgaaaccc acgcaggtgc ccgaccggac
    11581 caaggcccgt catgtacgca ttcccctgat caagacagcc accggcgacc tcgactatga
    11641 ggttgttctc aagtacggcg gccggcttgg ccccatcggg ataaccggct cggtgacgtt
    11701 tccggtgatt gagacggtga acatcgaggt agccctgagc caagttcgct tatatcttcc
    11761 ggaaactcat cggtggtttc gcttcggcgg gacgatgcgg caggcgggcg acgaggtcga
    11821 gctggcggcc gactacgtga cgtatcagac caggaaggcc cgccaactgg cagaggcgct
    11881 gagctcggcc gacgattacg ccaaggtccg ggcggccgag aacgtgagag ttctcggagg
    11941 gcagatgcag gagttccaga gtttcaacag cgcgattgtc caagagggca ggctacgaaa
    12001 agagctcctc tcgaacgcca tggtcatctc gcaggctggt gagcaggtca aagaacttgc
    12061 caaggtactg gaacaagggg ctgtgacgga caaccgcggg gatctgaacg attacttcgc
    12121 cggccaggcg aacacccgat caagcaatgt ggtcatggag ctgggcacca atttcgaaac
    12181 aggagacgcc tcagcggcaa cacttcaacc gggcggggac gacgggttca gccatgattg
    12241 gttcgtcgcc aacggcctgg tgagccccgc gccgcaatcc atcatctggg atgctccagc
    12301 aaccgctcct gctgaggtgc aagacctcgc cggcattcag ggtgtcgcga atctctattt
    12361 cagcgtgagg caggaagggg ctaaggccag gcaacctgcg gttccgcagg tgattcagga
    12421 ggcgcagatg gaggatcttc gccagagcct cgatagggtc caggtatccg cgagggaaag
    12481 aaagaaggaa gacgttctcg atgggaaagg cgacactctg acgcgttacc aggacgttgt
    12541 gcgtgagcgg gcatcggtgt cgcaacgccg ctcgagtatg tcgtatggcc atggtgtgga
    12601 gctgggccac aggagcacga taacgcttga cgcgatggat gatgccgacg gtgatgatgc
    12661 aaaggatcgt agccgatacc gcggcaccat gggcggcatg ggcatgggag gcatgggcgg
    12721 agggatggag gggatgggcg ccggatatgg cgcgtacgcc ggagggggcg atgtaatgac
    12781 gggcggtgca ggtgccggtg tcctgaccgc aagagtacct ggcgggttct cggcgaaagg
    12841 actggccagc ctcgatgtgg atatcccggt ccgcggccaa gtctacttct ttaccactcc
    12901 ccaaggcaaa gcggagatca ctgcccgggc ggtctcacgc ggcttcctga gcaatcttag
    12961 gtatcttgtc gtggtgctgg tactcgccgg cgcggccctg gcatgctatc gcgcgttggc
    13021 gcagaaggcg ttcacctgct gcaccagtcg gcgcggggcc gtggtcacga tcgttttggg
    13081 gctaatcgcc gtgatcgtgg ggttcctacc cgtggttgga ctgctcgccg tggcggtggg
    13141 tcttattgtg ttgatccgag cgcggggcaa agttgcagcg ggcccgctgc aggccacatg
    13201 attcattgag agcggaacga tggtagccgg cttgtcgtcc gcgtttttgt gggggggtca
    13261 gaccttgatg aagatccgcc ttctatagag cggcgcggtg agcagtagcc agagcagaac
    13321 gtaggtgatc gcccagagca gggaggcatt gaaggggccg gcccaggagg cgaagatggt
    13381 ttggtagagc caggttctca ggctgatctc gctgccgtcg gccaagtgcc atttgtatgt
    13441 catcatgagc cgtccgaaga tgccggaaac gaaatagacg gcgatcgcat tcgtgccgaa
    13501 taccaggaac ggccatgccc accggcggta tctcttcacg tcgaccagcc agaaggagac
    13561 agccagagac tgaagggcca gcccgccggt gaagagtacg tatgaactgg tccatatctt
    13621 cttattgatc ggcacgctgt agttcataaa gtagccgacc gcgatggcgg cactggccgc
    13681 cagaaacagg ccggcggccg tgtcgcgctt gtcacggctg ctgtgcagcc agttgccggc
    13741 caggacaccc agaaggcacg tggctatggc tgggatggtg cttaataccc cctcggggtc
    13801 ggggcgctcg ccatagaggt gattgcccag gcggaacatg cgaaggtcga cccattcgtg
    13861 gagcagacct tccggacgat cgggcggaag aagctctgcg cgaaaatcgc ccggcggcga
    13921 ggcatatctc agcagggccc agtatccgac aacgagaact gcggtccaga tggcccgacc
    13981 tcggacgcct gagtacatca cgatgatcga gctgaagaag tagcacaagg cgatgcgctg
    14041 caagacgcct ggaacgcgaa tcctggattc cttgaaggcg tcaaagcagg tcgcgaaggt
    14101 tgcaatcgcg ccgatcgtca gaagccagcc ggccaccatg cgcatctgtg gccacgtgcc
    14161 gcgaggccag cagaaggggc ctctgtcagc gaacatcaga gccaggccga cgatgactgc
    14221 gacaaacggt cccatgattt tccagcccga gggcttggcc aacgcgtccc aggcaggaaa
    14281 gcaggtgaga tagccattaa ggatcaagcc gagcacgaaa agaatgatgg ttcgacggac
    14341 aacctgcccg aacaaagccg gccggccggc cccttgagcg attcgccggt caaacgagaa
    14401 ggtcatcgcc acgccgacga taaacacgaa gaaggggaac accaggtcgg tcggcgtcca
    14461 gccattccag tccgcatgcc gcagcggcgc gtaaacgtgt gaccccgagc ctggattatt
    14521 gaccaggatc attcccgcga tggtgattcc tcgaaagacg tccaagctga caagacggcc
    14581 ctgttgttgg ggcacggata atccggaaag cgtcggggcg gttgtttcag ccatggggca
    14641 ccgtccccaa taatcaccgg gaaaaggctt cagtgcaagg tgcgggaccg gacgattcag
    14701 cgggccgggt tgtcttcggc ccttccgagc catgccggcg caggcgacgg acccgcccgg
    14761 agcagggcgg ggcctatttg tgccgaagaa gagcactgat aatgaagaag aacacacttg
    14821 ccaggacgat gacggcgccg gtacctacgt tggccaggta gtctgagaag gaagccagcg
    14881 tcccggccac gccgctgagt aagccgaagg ctattgccca ccagaacatt ccaccggcgc
    14941 ttcgtgcgag gttgcgggcg gttgcggcgg gaatgaccag cagggcagtg accaggagga
    15001 ttcccgcggt ccggatggac accgtcacga ccaaggccaa gaggagagta aacaagtaat
    15061 catagagccg cacgcggatg ccgcggctat gggccaactc gtcgttcagg ccgacgagaa
    15121 tcagcctgtt gaatccggcg atcatgaaca cgaggacggc gattcccaac aggaggctca
    15181 gtccgaggtc tgcgctgtcg aggttcagga tatcgccgta gagataccgt tggaactgcg
    15241 tcgtccgggc gccgcttcgg gtgacgatgg cgatgccgag ggccaccacg gcgctgaaga
    15301 acacgccgat gaccgtatcg ttcgacagat cggtggtacg gcgtacgtag gcgattccga
    15361 ggccgacgat caggccgaat agcaccagcg tcatgcgcgg atcggccgca gaaaaggatt
    15421 cgtgcagcag caggccgata gccacgccgg caaacgcgga gtgcgaaatc gcgtcggaga
    15481 agaaagccat tcggaagttg accactttga cgcccacggc ggcacaaacc ggagccaaga
    15541 gcaggcattc cagcagagca tatcgcatga actgatattc gccccacttt gtgggcagga
    15601 tacgacggag cgcctcgtca atcgccggca ggataacgct gctccagagg tcgatagtgg
    15661 ccaacgccgt actcatttgc cgcaactgcc cccgtgatga tgcgtgtggc cgtgagagtg
    15721 cccctcgtgc tcgccggtat gggcgtagag ggcgacttcc tgctggtaga gagcttttag
    15781 attctctgcc gtgagtgtct cgacagttct tccgctgcat ttcaccgttt tgttgagaca
    15841 cagaacatac tgggcatgcc gcgtgacgat gctcaggtcg tggctgacca gcagcatggt
    15901 gtaacgagaa tcacgttgca gggaggccag gaggtcgcag aacagttcct cgccggtcac
    15961 gtccacgcct gatacgggtt catcgagcag caggatttcc ggttcgtcgg acagggccat
    16021 ggccaggagc actcgctgca attcgccgcc cgaaagcctt ccaagcggct ggtcggccca
    16081 gtccttgccg ccgacgcgat ccagccaggt ctgggccaat tggcgagcct gcctgtggtg
    16141 tccgagccag acgggagtgc gttgaaggcc tgcgcacatg aaatccatga cggtcatcgg
    16201 gattccgcga tcgaagtcca agcgttgcgg aacgtagccc acccgcaacg gcttgccatt
    16261 cctgcttgca ccccactcga tgcgaccggt atagggcacc aagccgagaa tggcccgcag
    16321 cagcgtagtc ttgccggctc cgttgggacc gatgatcgca ttgatctgcc cgacggcagg
    16381 ctcgaaggtg atatgttcca ggatcggtcg cgcaccgagc gtgacgccga cgttcgtcaa
    16441 gcgtagtgcg aaggtgggcg ccgcgttgtc gggaaccgcg ttcaaggtca tgatccaagg
    16501 gcctttacca gcacttcaag gtttgcttcc atgacctctt cataagaccg ttctgttgga
    16561 tcgacgtcaa ggctgttgaa ggggttgagc gggaaggcgg gtacgccggc gtcacggctg
    16621 atggtgcggg cgaccgcatc cgaataggcg ggctcaaaga acacggccgc cgctcccgtc
    16681 cggcgtatgg catcgatgac ctccgtcatc tccgccgcgg aagggggcct tgatggatcg
    16741 agcgtcaatg tggcaacgac ctcgaggtca agatcctttg ccaggtaagc gaaagcgtcg
    16801 tggaaagtca caatccggcg gttggcgaag cgaacagatg cttcccgcag tcgccggtga
    16861 agggcctgga tgcgggcaac gtaggcttcg gcattggcct gatagcgagc ggcgtattgg
    16921 gggtctgcct cggagagccg gtgagccagg tttcggatct gccgtatggc ttgaatgggc
    16981 gagacccata cgtgagggtt cggttcaccg gcggcatgtt cctcatcgta atggtcgtca
    17041 gtctccgggg caggggcggt tgccgcaccc tggtggtgat tgtcgtgctc gtgttcgttc
    17101 cggttgggtg cggcttgacg gtccgtcgcc ggtcttgaag cagccgtttc tgccgagtgg
    17161 tgcttgtgat ggtgcgaggt ccggctttgc aggatctcac agtcgtcgga aatggtgata
    17221 agcctggctt tgggattggc tttctgcaac gtatccagaa acgattcaag cccgagtcca
    17281 ttggcaacaa tcaagtcggc ttgcgacacg gccttgagat ctgcgggggt cacaacatag
    17341 ttgtgggggc atccgacagc ggtggatacg agcaggcgga tatcgacacc cggggtgtcg
    17401 ccgacgacgt tgcgggtgaa cacgtagttg ggcaggaagg tgcagacgac cttgagcctg
    17461 gctgcctgtt gggagtcgcg tccgctgggc aagaaaacga cggcaaccac tatggccagt
    17521 atgaccgcaa tcaggaggat ggttcgccag tgcgcgcccg atgcaggttt cattctcgtt
    17581 cctctcaggt gcttgcgaac gatgccaaca cggtttggcc tcagggccta cgggtttcac
    17641 ccctttccat atcataggcg cgcgtattac agtgtcaact ggctcgtgat aaaggacttg
    17701 caggcggccg gttaatgaag gccttccccg caagaagtcc tgcgtcccgc gcacgtgtct
    17761 tcgtgggagg gtttcgcggg ttgcacccgc cgagcggagt tggcctcaat gaaggtggtc
    17821 gtgctgttca cagtgggggc agacgaaacc ctgattgtcg gtcttgcccc gttgagtctg
    17881 ggctatctct gccgggtagg cgaaatggca gcaggtgcac acgacggcct cgaagtcggg
    17941 cgtatgggcc ttgagccagt cggcgaggag ctgcggaagc acgccccggc cccggcaaaa
    18001 agggcagacg gcatgaaacc gctgaagaat ggcgtcgcgg ataaacgtgg acttgtcggg
    18061 gatatcgttc aggatctcgg ccagatgctg atcgacacgg aaggaaacga cggtctgttt
    18121 ggattggtct ggcataagag caactcactt cccgctattg gcgttgcagg ggttattttc
    18181 cgttatcata gcagaagcgc ccggattgtg cccgggggac aaccctgacg gggcattgcg
    18241 gcggcgtagc aggaaagcac aatgcccaag gaaccggacc tgttcgagat gtaccgctgg
    18301 gtgctgatga tcatctgcgg cgtctacacg ttggcctgcc tggggcagac gttggtcggc
    18361 tggctggact atttcagcca gtcgaggcgg acacaggttc tcggccacta tgcggtggtg
    18421 ttgcttctga gggcaaggtt gcgacgcttt gcgcgtgacc tgtggcagat cggggtactg
    18481 ttggcgatct ttgttgggct ggtctatgct catcgcggct tttgagacct tacggcctga
    18541 gagacctgtg tttgacgcgc gtgtttatga tggccgcact ctttggtttg cggctcgcaa
    18601 ggcggcttgt gtcccgagtt gacgggggta gcgggaatca tgagcgaaca gcaaggcaaa
    18661 tcggctgcaa ttgacgacct gccggttgac gaactggtcg cctacggtcg gacactcggg
    18721 atgcacctga acgcgaacgc cggaaggggg gagctgctgc gtctggtgcg cgagcggcaa
    18781 gcgctcctgg cagagttgga gcacgaggtc ctgttggaca tcgtcgtctg ggcacgccgg
    18841 ccggtgcgcc aatcagccag caaagaggag cttgccaagg aaatcgcctc cgtcaagcgg
    18901 gttgattttc agggcctgag cgacaggggt ctggtggccc tggccagact gcgaggcgtg
    18961 cccgtctcgc ccaagcagcc gcggaaagaa ctcgagaaag ccgttcgcgc gagcgaaccc
    19021 ttttgggaca agatcacgcg caagcgacgg cggctgatcg ggtcgctcat cagtcacctg
    19081 cttgacgatg cggagcctga atcgaaagag gcctatcgtt ttcttccaga agagagcggt
    19141 catacctcgc tcaaggagcg gatcgccgag caaggggtgg tgagcggcct ggctcggact
    19201 attcggggcg tggcagacga ttacgttcgt caaaagatgg acgagatcga agccaggatc
    19261 gatcgcaagc tcgatgagat cgaccggcgt cttgcggaat ggcgggaccg tgagatcgcc
    19321 aaccgcctca agatcatcaa gatcacgctg gtggcgtcga ttcttgtggc gctgatgagc
    19381 tacggctata ctctgctcaa gtcgaatatc aaacctgttc ccgccggcgt ggaggggatc
    19441 cccgtgagcg cgcctgcggg gggtgcttta acaaggccgg ctgaggcgga ttgactgata
    19501 tgtacgagcg gttggacaag gggctggagc gggtcataaa agtcgcccac gagattgcgc
    19561 gggagtatga gcaggagtac gtgggcacgg aacatctgct tctagcgatc gcccgagaga
    19621 gggaagggct ctcgggtcaa gtgctggcgg aggtgggtgc cagtgaggag aaaaccaagg
    19681 ccgccgtgga caggctgatc aagaacagcc ttgaggatac atgggttttc gggcgtttgc
    19741 cgggtacgcc ccactttcgc aatgtgatgg ccaaggcgat tcaagaggcc cgcaatttcg
    19801 gcagcaagga agtctccacc gagcacttat tgatcgggct gctgctcgaa aagggctccg
    19861 tgggctacga ggcccttaag tcgctggggc tgacgcccaa gctgatccgc gacaagatcc
    19921 gaagactcat ggccgagggg gccgtggact gatctgccgg cttgtgcctc gccatgtccc
    19981 cagtttccct gcaggcatca ttcggttcgg atggcaggac gacagaactc ggtctggaaa
    20041 gctgctgaag gaccgcactt gcgcaccacc aacgtctgtc ctgccggccg ggcttgatgc
    20101 cgcctcgctc ctcctgtgcc gccgggcagt tctgctgtgc gtggtctagt ttcaagagcc
    20161 gacctataag tgtttgagga tgtgcgtatg agaatctttg cggacggcgg atagaagaag
    20221 gaggttgacg atgtcgcgct tggctgaact gaagtgcgag gcatgccgta gagacgagcc
    20281 gtgcgtcacc agggcggaaa gccttgatct gttgaaagca atccccgact ggcggatcat
    20341 cgaatgtgac ggcttgcagc ggctggagcg tgtttacacc ttctctaact tcgctcgggc
    20401 ggttgatttc accgaccagg tggcgtccct ggcggaggag gtcggccatc accctgcgat
    20461 tctgacggaa tggggccggg tgacggtgca ctggtggacg cacaagatca agggcctgca
    20521 ccggaacgat ttcatcatgg cggcgagaac tgacgaggtc tatgctgggc acgtccggtg
    20581 agggctacgg ggcgactttg acaatcacct tgcgcacggg ttccggcttt tccaccgcca
    20641 ggccgggcat gtgaatgtct tgcagggtaa agacggcgaa catgccctct tcgacgtcga
    20701 agaaatcgcc gcgggtgttg aagaagacca gatccgtctc agggttgtaa ggttctctga
    20761 tcgaagcacc gttcattcgg gcatatccca ttcgctcgcg gcctcgtacc acatactgaa
    20821 catcccagta cttgtggtgg gcttcccact tgccctggtt cgcaggcttg gtctgattat
    20881 cgcagacaaa ggcgaagagc cggtcgccgt cgatcggata tcggccggcc ggcagcgtcg
    20941 cgagatcggg acgccgaagg aaatcgatcg ccttgggcaa caggggatga atggtcttat
    21001 acaaatccgc gttcttgaga gaatcaacaa tcatgatcga gtgcctcttg tcaaaagctg
    21061 acgcggggct attctaagcg gttcttatcc gtttgtgaat tgttgccgga caagttggtt
    21121 gaaatcggcc gcaagcccgg cttgatcgat cccgacgagg cgtccgtcct gggccagggt
    21181 ccggcctgct acaatgacgc gcttggggcg ggaaggctga cagagaagaa gagctcccaa
    21241 ggcatcttga gccgcgccgc cggccatcgc gatgtcagtg gcatcgtaca aaaccaagtc
    21301 ggcagcgcaa gcgggctcga ttcggcccag tttgggccgt cccaggaccg ccgcacctcc
    21361 ggccgtcgcg aggcgaaatg catccgcggg actcatggcg cccgccccgt tgaccactcg
    21421 ctgaagcagg agggcctgcc gggcctcggc cagcaggttg ccggcgtcgt tgctgctgct
    21481 gccatcgaca gccaagccta ccttaatgcc ggcatcgagc atcttgcaaa ccggcgcgat
    21541 gccgctgccc aaacgcatgt tcgagcaggg gcaatgggcc acgccggtcg aggtttctgc
    21601 gagcaggcgt atctcggctt cgtccacatg aacgcagtgg gccaggtaga cgttcggtcc
    21661 cagccaccgg tgttgcctca ggtactccag cggccttacg ccaaaccgat ccaggcaata
    21721 ccgctcctca tcgagcgtct cggcgatgtg cgtgtgaagc agaacgtcgc gctcggccgc
    21781 tagatccctg gtctggtcga gcaacgccgg cgtgatgttg aacggcgcgc acggggccag
    21841 gtcgatccgg cgcatggcca gcggcgaagg gtcgtggtaa agctcgataa ctcgtaccga
    21901 gtcggccagg acatgctcgt cggtttccac gcactcatcg ggtggcaacc ctcccgccga
    21961 tcgtccgagt gtcatgctgc cccggcagaa atgaatgcga atgcccaggg tctcggcggc
    22021 tctcaacacc gcctcagcgg cgacatcgct ggccggtggg aacagataca tgtgatcgga
    22081 ggtcgtcgtg cagccgttga gaatcaactc cgcgatggtg atcagagcgg ccaggtgcag
    22141 ggcgtcatag gtcagatgct gccagcgcgg atacagcccg acgagccagt cgaacaggca
    22201 agcgttttgt acgtgcgggg cgcaacgggt cagtgactgg aaaaggtggt ggtgggtgtt
    22261 gatcagcccg ggaatgacca ggcagtcacg gccgtcgatg acggtgaggt cgttggcgcg
    22321 ggccgtaccg gcttgcgaga taccgccgga ctgtatggac agcgcgtact cgatgtcagc
    22381 ctcactgacg tagccgaggt gaagcagttc ctggccgatg acgccgtggc tttggccttg
    22441 gatttgcagg gcttcctgga tctgttcctg ggtgactttg ccggtcgcca acaggattgt
    22501 cccgagcggc cgtcccttga gttccggagg cggttggttc gtcggccggc cgtttgtttc
    22561 agtcacgtga tcggtcggtc ccacccccgt gataacgccg tcgcggaact cgacggaatg
    22621 gtctgacagg acgaccggat catttccgcg gcaggtgatg atcgttgcgt ggtcgattcg
    22681 tgttcgcatc ggctgacagt gtagcccgag acggtgatct cacaagcccg gccgacgggc
    22741 atagagacgg tagggtgggt ttcgtccacc gatcacgtcg agcgaccgga acgtctgcgg
    22801 cgtccattgc cgttttgcgg ctcgatcatc gaaatgtgca gaaggttgat gatcatccaa
    22861 tcccgagcga tggcgtcggc agaacgggga gtggccatcg ggtgcgagga ctcgattcgg
    22921 gccaagccga cgagaagatg gcgctcggca acaacgacct ggtccggatg gcgaacgagc
    22981 acacaccggc cgtccgacag gccgatccgc agaggttcaa agggttgtct cctcagaagc
    23041 ttggtgatcc cccaggcgcg cacagcgccg cattgtatga gtgtttcttt ccgcgagcaa
    23101 actcgtcaag gcgggttctg ctcgccgatg accgttcggc gaatgtcacc ggccggaaga
    23161 ggaggcgttc aaccccgcgc gccgacgacg cctccgccgg cgcgacaagg tcctggcgac
    23221 aactggtggc atgaacaccg ccgccgcagg cgaggcaagt ggtatggctt gaacaactca
    23281 tcgccgatcg gtggcccggc tcggtgactg gagttgtcac tattattccc catgatgatg
    23341 gcgatgattc gggtcgtgtc ggcacggcgc aaactaaagc cgtgtggtat tccggcggca
    23401 ggagaatcgc cctattggca ggggcagacc gggacgaggt agaataatga ttttgcgagg
    23461 tgagcggaaa ccgccggatg ctgaaaccgg cgctgcgccg tttgggagac agacgctgat
    23521 ggcccaagtg ttttgcatag ctgcgaagag ttcgccaccg ttgccgacaa ggacgtatcc
    23581 tcagacagtg tgggttgggt ttcaggtccg accggccaga tgccggtccc gcaccggggc
    23641 tgcgggtttc agaggagaca ggcttgcccg ggcgtgggcg tggcggcgcg ggttcacgtt
    23701 gctggaggtt ctaattgtcg tagcggtggt ggcgctgctg gcggcgattc tggtgccggc
    23761 gctgtcggga gcgagggatc aagcccgcag cctggcttgt cgttcgaaca tgaagcagct
    23821 catgaacggc gtgtcattct atgtggcgga ctaccacgta ttgccgggta cgcacagtct
    23881 tttctttttt caagctcttt tcggcgcggc ctggccccgg ccgtcggggg tgacctggga
    23941 cggagccagg gaccggctgg tggggctgac gttcaaagcc gcttatgccg aaccgcacca
    24001 tctggatccg gagttcattg ccgacgtacc ccggaagggc acgatcttcc ggtacacgaa
    24061 ggaggagcgg atctacacgt gcccttcaga caggcccggc aaggccgagg acacggccct
    24121 cggcggggga ggcaacggcc ggctcgggta ttcgctcaac gcttacatcg gttacaaggc
    24181 gcctgagcac ctgcagagct ttacgtacgt cgcggcatcg ccggacaacc gcttgccggg
    24241 cggtcagaaa aggcgctcgt ttgcggccgg gcagcgggtg gtcttttcgc ccggggcctt
    24301 catggtgctg ttcgaggaac acccttttta ccacatgaac acgagtttcc cagaggggaa
    24361 cttcaactgc ctggaccgga tcgccacccg gcatctgctc aagggcgggg ccaagggcgg
    24421 aacgcccgag gggcggacga gcgtggtgtt cctcgacggc catgccgaaa gccgcctgta
    24481 cccggccaag acgatggggc gggagctgtt tgccgagttc ggccagccgt acgtctggcg
    24541 agagtcggga ccgccggaca cgacgaacat gacggccttt atcacaaggt tacgcgggcc
    24601 gtgcccgtgg tgacaggcca ctaggccctg atcaagcata ctccggcgag gttttctcgc
    24661 tttgttcgta aaaa
//
LOCUS       DAOTPW010000060        24311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2149_length_24311_cov_5.287475, whole genome shotgun sequence.
ACCESSION   DAOTPW010000060 DAOTPW010000000
VERSION     DAOTPW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 24311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24311
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2149_length_24311_cov_5.287475"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="P5316_07385"
     CDS             <1..375
                     /locus_tag="P5316_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84818.1"
                     /translation="SARRSLCRRGRRHYLGRRRCFWANTLYWPIGFVPTRASALLWVS
                     ALLGALALLGVSALLGVSALRMSPVDGVGEKKFVPTRASALLLGQYPLLADRFCADEG
                     VGTTLGVGTTWGVGATWGVGAT"
     gene            complement(368..3370)
                     /locus_tag="P5316_07390"
     CDS             complement(368..3370)
                     /locus_tag="P5316_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84819.1"
                     /translation="MQKWLCACVLVFAAIANAADNGPWKLVFEDRFQRAELGPDWVEA
                     KNVSIVHGRLFLKGRADARLRRGFPGDVKVEFLAEADPDQPPCDLAVTLANTFLLAFG
                     GGNNTFNQIMGAGVTVRDNKPPFPIEHGKKYKIVAVKEGPMLILYCNDHKLLEARLDD
                     PLGGPEFDGVALTTWNGMYVDYVKVFERTTPAPGGPVVLRAMPALGFTWKDRQLSYSG
                     PAALTPNVTQGIRLYNERKYQEAFATLSAVSPATLESAAALAYVVGDLAFLEKPAHQA
                     QVARVAAELAARAPEDTKAKDFALAAKWFADFNVYRRDHRATRRLIGCGPDNNPFYYK
                     ARLFENRFNYAHAIEGAHREGMQAAINEFAELKKIWPEHQALREWTGEKIPWGEELIR
                     PESDGPAWARHLQESLARQQAVLRWWFSERQLPDGQLGGGWGDDVEILRGWVPIACIT
                     SAAEPVIAGIERLAQGVWDHVLKDGYDPFPGDVEHSAEPSCDSLPTMIVLRYGDPLWL
                     ERNLRSAKTVREKFMDLNERGFLQFMSRDFGTDGVKRGLGNGGDSGYHARATKHFIYL
                     GWYGVPEGRDAFVQWCDTWRDATMRQIGPKPAGFPPSTLWFPSGGIAPPDGKPWYDEQ
                     RNSYGFPGLPLMIYESFTTAYLLTNDRKFLDPVQTMMHMATAGPLHKGDPKLPPDHIE
                     NLMAQWSHMASADVLSAYRLLTGERVYDEYILRMATPTQRYMTDYDLARYVTTFEKTA
                     NSLRHNWTQLTSEILQTDRAGLTGSAEVFGAYTGAVCSFRDSKPPTISVTYDTPDLNF
                     ASVVTETTPARLRIMLYNFNAEPMRVGLKPWRLVPGVYVLNAGEPVPGEYDFQKRYTW
                     TEPTEVEHLHRGTPIWVEIPSHKEWVVDLRLRKAIERPAMLPDLAIASRDIQLKDGKV
                     VVRVHNIGGAAAGPFDVVAQAGNGDTWTQLAHAKVDGLPAIKSFEPSVREIVVNVDPD
                     KLANARIRLDPQNQVQELYELNNEATLK"
     gene            3610..5397
                     /locus_tag="P5316_07395"
     CDS             3610..5397
                     /locus_tag="P5316_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRR84820.1"
                     /translation="MQNSLNPFIMPHNRSHPISVEEFMMAGTLNAPTEPVLARRRPRE
                     NWGSRLGVILAVAGSAVGLGNFLRFPGLAATNGGGIFMIPYFISLLVLGIPICWVEWT
                     IGRYGGARGFNSAPGIFSALWPSRVSKYFGALALIIPVGIYMYYVYIEAWCLAYAWDY
                     VSGTMTRYGADAGTDVTAAVAAYRTHFTEFVGMGENGLLVYGSLQKGVVFLLIVFVIN
                     FVLIYRGLTKGIEAFCKYAMPLLAVFALIVLVRVLTLGAPDSSRPEINVNNALGFMWN
                     PKTGDEGFLAALANPELWLSASGQIFFSLSVGFGIVLNYASYLKADDDVVLSGLTATS
                     TNEFCEVCLGGLITVPVAFLFLGEANLTKDVLGSTFSLGFMSLPAVFARMPAGSFFGA
                     VWFFMLFIAAITSSLSMLQPAIAFFEEGFGMGRRLSVTCLGLLTAIGSLLVVYFSKNL
                     LATDTLDFWIGTVCIFMLATVEVILVGWVFGVGRAREEVKRGAALEVPRVFWFIIKYV
                     SPTYLLVIFGFWCYKNIPAKIDEVRAMSPEDRGTVLLMLAFIGVLLIFFGMLVNLADK
                     RWHLIGKLPLEPARGLPLAAADEEGRTRS"
     gene            5394..5546
                     /locus_tag="P5316_07400"
     CDS             5394..5546
                     /locus_tag="P5316_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84821.1"
                     /translation="MTWAGWLLMTLSCGVVVSLMVFCYRRVLRTPRPEEHMHAPLEID
                     TRDADT"
     gene            complement(5561..7087)
                     /locus_tag="P5316_07405"
     CDS             complement(5561..7087)
                     /locus_tag="P5316_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR84822.1"
                     /translation="MRIALINPVARRCQGYHTIGGRIPQLGLQVLAQLTPSDHQVDII
                     DEIFGPQATERLVRRGRYDLVGITSYSSGASRAYEIAAQARREGIPTIMGGPHVWAVP
                     DEVAGHVDSIAIGECDEIWTEILSDAAAGRLKPRYQGRPASISTPGIGRGAQHLQAVN
                     GSYTVAAIQTSRGCPVGCDYCSVTLFSGPEIRRRPIDDIIDEWNSTIKRFIFVTDDNF
                     FGVGPKHAEWAKELLRQIIKRGKKRLWFSQTTINMGADAEGLDLAYRAGCRGMLIGFE
                     TFNPESLRDYHKGINRMNLSRYKELVDGFHRAGIPVFGAFIIGADQDDENTVAETAIQ
                     SVKLGIDTIQITNLTPLPGTRLYQRWMSEGRIFATNYPEDWERYTFVETVYHPRKMTA
                     RKLDETIYELRHAAANTPWVWRRTLRTLLATRSLTGSLFIHGMNKGWKRIARMQLPKD
                     EERFGFIPHENERTRKLRDAFTMSLRKYQAHRSMQPAPSPAPLNPVCPAKLIERSREP
                     "
     gene            8234..9943
                     /locus_tag="P5316_07410"
     CDS             8234..9943
                     /locus_tag="P5316_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR84823.1"
                     /translation="MTDRLFPIFEDHRRVGLTVVLAFCWLTGCSSVFPTKVEPLLDPE
                     SLRPSPQVWQLSDPLDPSKPATQPMATRPAGLAATRPAAGPSTRPATGELPSPPYRIN
                     ADSIPRLVYHKYPLVTSAREQMIAAQHGLQEFKANLSRFEPFTNVSGMAFQYPERRDA
                     SGLSGEVTGGIEKETFDGAIYRVEGGARGERIKYGEVGEGQASEDSGEGGLVRGRVEV
                     PFIGSRKRQDRTINQAYQESTARAAVLNYLSYYRSYALTAMNYYAGALLYLGYARAYE
                     HQIEALEDLLKEPGLTPQDQQVILTSIGSSRIVRDSYKASYQSYLLWTLEYLGIRPGE
                     EYYLEEPPVSEGSIYYDRTRTDEQRQELLTEAYESNPRFRVLNDAIRDAELKRSQAIL
                     GKNDITAFVEGTQHAFGAETFDDRVGGWELRGGVSFRMNDPRVLNASIRKAEAEIRSY
                     QAQIEAEELSVQQQIAVQSATLTTYVESKPQILKNIDEARSEFEARRKAYFAGQSTLL
                     RIDDVLSSLQSITTAEVRLVSNRYYTALADNMLMSASGQLYQMAGVKLTEDAGTVGLA
                     EKK"
     gene            10430..11848
                     /locus_tag="P5316_07415"
     CDS             10430..11848
                     /locus_tag="P5316_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR84824.1"
                     /translation="MAYLISPLVIASREDMMAARHGLEEFRANLSRLEPYIGVDGGAL
                     EYPQRRGVNGYDSELVAGIQQETFDGSVFRVEGGVNGSRYSYDRTQSDAAKTESGSSG
                     VLRARVEIPFIGSRKRQDRTIKQAYQESSARQAMLEYLRDYRTYVNNALAEYRQTILY
                     LNQMRAYEDHIAAIEALWDLPELTDDERTRLQTALGDAKVHHSGLQASYWSSLLSLLE
                     TLGIQPGEKYTVEEPKELSLQFLDEVSTPEGRQRLLAKAFENNPRFAVLEDAIRNAEL
                     KRSQAVLGTHDMTAYFEGAHYPFAPPEFDDRVHGWLLTAGITVRINDRRVLTESRKKA
                     EAEIRAYRAQIAAEENDIRDQITIRADRLLTYSKSRSEAIENVEQARAQFQERCEAYF
                     WRGESGMNIDDVLRSLSVLTNAQVQLASNFNQIIDAEHDLLVATGEVYRLVGIRIEKE
                     DSGTVLHEALLSQPSVTSSQPE"
     gene            complement(12059..15397)
                     /locus_tag="P5316_07420"
     CDS             complement(12059..15397)
                     /locus_tag="P5316_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84825.1"
                     /translation="MSSRILIFVYVLLPAALAQGDWELYVNKFCCQNADAEAKWDANQ
                     DTDVWNDGPTLWVYVQCYFNDNDWQNNYKYKMRFSGGENEAAEISHTTQGCSGDYYGT
                     TLSQKLSTGGNRYYKDYDLTIYAQVRNTDGQQLDSESNTMTFRFRDTSWKIPTQTDIT
                     SPVSSVYKWSDIKVKGTVKRKPEEGGNWIAMDNHYKVKVELDHAGNHYEATVQTGNGG
                     FEHTFAQPQGGWDSRVSVYVYATFLSGQFDPADPPGCYVIEYQSSSDSGTYEVTDKRD
                     EAKSTWESLIQATGTITIQNPTDDALPDGSTWAYDLDGTLLGWQSTMAPPPHGVIVID
                     EDTLDDDLTALAGTIAMDQMIPGYETWAAPGRRGTAAAPGQNQPGPQAWIATLAFQLP
                     SGPMFGGEGLGWRYTTAWVYDPPESTALLTMRFYDMSGRLLHTKQATVNAYQRFEVPK
                     SWLTGAAAMHAELSQGHLLGWALAEDLTYDAFETTAQTSVAAELIAPYVTSEADWQTM
                     VILHNPTDSAALVVMEWYHIGGAFLGSLPELIAPKATMTFDADHLGPPALAKSVRVRV
                     LEGEGIIGSCVRTTNEATTMAESMVRPASSLRIPSPGVPLLQDGLLVMHNPEEEPVTI
                     DLRLYGLDGTPQGGAPMELAPHMTEILPLIPPLPGQPVPSFEMRASGGGRFAAIAMFH
                     QPDSGGAASVPALPMSERFRCGPPWLQGGWNLISIPLDSDNPNVTAVLAALEQAGNAL
                     PGHLLRYRADAYQAYPDEMTTLARGEGYWLRLDQASTFSVRGKAAKQNVLIGLTDGWN
                     LIGCPTDVPVLWAACSVSDGSQTKTVTEAGQTGWIQSTLLYHEPVSGYRLVIPGETGD
                     DAYLRPCQGYWLRAFRHGLILVVPPEGGAAGILDAGAETGSSLGIQITGQATATWSFR
                     AAVDPAASDELDALDLMAPPAAPAGLAELRLTTTPDFQGPRIRDYREPPCGSDEVWEA
                     TASLAGLGAGHCEQATMTWDLSNIGSYSYTLVDVTGQQNVDLSPGGQYLFRICEGQDV
                     SFEIKAKRTGLTPADLDCDADVDGADADLFENCATSPGVSLSPGCDRIDFDQDGDADQ
                     TDFAFFQRCCSGENVPADPSCAER"
     gene            complement(15609..16850)
                     /gene="fabF"
                     /locus_tag="P5316_07425"
     CDS             complement(15609..16850)
                     /gene="fabF"
                     /locus_tag="P5316_07425"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRR84826.1"
                     /translation="MAKRRVVITGMGVITPLGCSVREYWDGLISGRSGIGPIQRFDAS
                     AFDTHFAGECNSFNPEQWIEPKAAKRMDRFAQFAVAAAIDAAQDSGLDFSRINPHRVG
                     VITGSGIGGIQELEDQHRRLMDKGPSKVSAFTIPKLMVNAASGNISIRFGTRGPSTAV
                     ATACASATNAMGDALRLIQRGECDVIFTGGTEAAVTTLGLSAFNAMKALSTRNDEPTR
                     ASRPFDRDRDGFVMAEGAGILIFEEYEHARQRGARIYAEVRGFGCSADGSHLTQPNET
                     GDYAAVAMRDCLNDSGINPDELDYINAHGTGTVLGDTAETLAVKTVLGEQSRKVAISS
                     TKSATGHLLGASGGVELVAAVLAIHLGILPPTINLDNPDPQCDLDYVPNVAREARIRT
                     AMSNSFGFGGHNACILVSRLD"
     gene            complement(17032..17292)
                     /gene="acpP"
                     /locus_tag="P5316_07430"
     CDS             complement(17032..17292)
                     /gene="acpP"
                     /locus_tag="P5316_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRR84827.1"
                     /translation="MTVQEIEDKVISIVSEQMGINKAEITREVSFINDLNADSLDIVE
                     LVMELEDSFETQIPDEEAEKIKTVGQAIDYIVAHQGQQQQQQ"
     gene            complement(17405..18151)
                     /gene="fabG"
                     /locus_tag="P5316_07435"
     CDS             complement(17405..18151)
                     /gene="fabG"
                     /locus_tag="P5316_07435"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRR84828.1"
                     /translation="MSWTEQVAVVTGGARGIGRAICLGLAQQGATVLAADLNFEGTKA
                     VADEAKTNGLPGKIVPKSLNVTDRAAVESFVEEVIQQFGKISILVNNAGITRDGLLLN
                     MEDEQFDTVINVNLRSVFLMTRAVCRHMMQARYGRIVNIASVSGMMGNAGQANYAASK
                     AGVIGFTKTVAKELARRNITANAVAPGFIDTEMTKVLPDKVKDMVKQLIPCQKMGEPA
                     DVAAGVIFLASPQAKYITGQVLVVDGGLNM"
     gene            complement(18249..19178)
                     /gene="fabD"
                     /locus_tag="P5316_07440"
     CDS             complement(18249..19178)
                     /gene="fabD"
                     /locus_tag="P5316_07440"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRR84829.1"
                     /translation="MAKTAVLFTGQGAQSVGMGRDIAQASPAAAAVFERANQVLGYDL
                     RALCFEGPAERLEQTDLQQPAIFTTSAAIWQALQERAVLTEPVAAMAGLSLGEYTALF
                     AAGAVAFEDALRLVKRRGELMQEAATAVPSGMVSAMGLDPDQVEAICRQASDAGVIQP
                     ANFNSPGQIVLSGAKAACEKAALLIEQAGGRAVPLKVAGAFHSPLMKPAADGLAEMLA
                     VTPFETPRVPVIANVNCQPHGDPDTIRRRLTEQLTQPVRWQESMQKLLAEGVQQFIEI
                     GPGRVLTGLMKKISRRTPIINVSTAADLALKAA"
     gene            complement(19191..20183)
                     /locus_tag="P5316_07445"
     CDS             complement(19191..20183)
                     /locus_tag="P5316_07445"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRR84830.1"
                     /translation="MGERIPLAIRGMGSALPDRVMTNDDFARILDTSDEWIRTRTGIR
                     ERRFADDHEDSLTLATTAAKRAVDDAGLTPADIDLIVFATSTPAYPLPATACFLQHAL
                     GCRHVPAFDIAAACAGFVYAFVAGAGLMQIGGYRHVLVVGSEKMSAITDFQDRSTCVI
                     LADGAGAAIIGPPDNELSGLYEHMLGADGSGARLIMVPAGGSAMPATAKTVEDRRHYM
                     KMRGREVYKFAVTKMQEILIETTRRAGLRVEDLAYVVPHQSNLRIIESAAQKMGLPMD
                     KIAVNIDRYGNTSAASIPMALEEAWRAGKVKTGDWVLMAGFGAGLTWGSALIRL"
     gene            complement(20191..21207)
                     /gene="plsX"
                     /locus_tag="P5316_07450"
     CDS             complement(20191..21207)
                     /gene="plsX"
                     /locus_tag="P5316_07450"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRR84831.1"
                     /translation="MRIGIDAMGGDYAPREIVRGAVEASAKLNGHQIVLIGDENVICK
                     ELELAGGAWPNISVMHTPQVIGMDESPVEAVRHKPDSSICRMAQMAAKRELDAIISAG
                     NTGAFAAASQLRMKLLPKVARAGIAVVLPSFAGPIVMCDAGANLTPKAHHLVQYAIMA
                     GAYAKHVLKITRPRVALLSVGQEDVKGTSLVKQTHELLDSCPQVNFVGNVEGRDLFRG
                     GCDVVICDGFVGNIALKLIEGLAEGLFKTISKEIALASPELARHFEPVVKRVWANHDY
                     AEYGGAPLLGVDGACIICHGSSDHVAIRNAVRVAAEYYENDLNSIIAAELVEEPAAQR
                     LSDK"
     gene            complement(21213..21398)
                     /gene="rpmF"
                     /locus_tag="P5316_07455"
     CDS             complement(21213..21398)
                     /gene="rpmF"
                     /locus_tag="P5316_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRR84832.1"
                     /translation="MLPARKVSKARKRKRRAHHALRRPNLVPCPKCNVAKLPHASCEN
                     CGYVSGDVALAIEEEES"
     gene            complement(21817..22470)
                     /locus_tag="P5316_07460"
     CDS             complement(21817..22470)
                     /locus_tag="P5316_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR84833.1"
                     /translation="MPKVLLINRDKATLGLLEAMFRPHFEVKKTTEVTAGASLLTKSR
                     PDLVVVGQEAKSDDALRLATWMRDNGVQLPVVAVLGPKADPVQPKLHKLGVRAFVEHP
                     ITKQQVQEAINTAVRFHKTRQAAPPPPTLEELRANLSELENRLNKAMKCFAGTNKVFI
                     LSQIGSTSRPRICLKCPLRPEYGLPPNVYYEFIRDVCCGDPNKCEAYYSFQTARESA"
     gene            complement(22779..23249)
                     /locus_tag="P5316_07465"
     CDS             complement(22779..23249)
                     /locus_tag="P5316_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HRR84834.1"
                     /translation="MSNPEKRAPILVLGIGNILLRDEGVGVRLIEAMRGMALPPGVEL
                     VDGGTAGADLLDIISNRRKVFVIDAVDAGLEPGAILRLHVEDLASAGRPLVSLHEIAL
                     TEALAAAQIIGSMPDEVIIWGVQPKSVSPDDTLSEEVASAMPQLIERIIAELAR"
     gene            23459..24280
                     /locus_tag="P5316_07470"
     CDS             23459..24280
                     /locus_tag="P5316_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRR84835.1"
                     /translation="MGIRTGKTVLKRTPTPQVTRTHYLVDKNAPLAQVVAETDESGTV
                     KVKYVHGDDLISQTRDPNGPSPITSYYHYDGQMSTRHLTNDPADPQTTSAQVTDSYTY
                     EAFGALWAHTGETPNDFLYTGQQLDPNTGFYYLRARYYNPAAGRFVTRDTFDGLKYDP
                     ITLHKYVYAASQPLTYQDPNGAFPSLLGTVGVISILLIVMSFPNVVNAPSIWDTTQPD
                     ASGEMVIGLGVSLILGLVVLGIGGLISRADRLFAGRNPSVLRSGNMRASRTDVRP"
BASE COUNT         4375 a         7366 c         7708 g         4862 t
ORIGIN      
        1 tcggcgagaa gaagtttgtg ccgacgaggg cgtcggcact acttggggcg tcggcgctgc
       61 ttctgggcca ataccctcta ttggccgatc ggttttgtgc cgacgagggc gtcggcacta
      121 ctttgggtgt cggcactact tggggcgttg gcgctacttg gggtgtcggc gctacttggg
      181 gtgtcggcac tacgcatgtc gccggtcgat ggcgtcggcg agaagaagtt tgtgccgacg
      241 agggcgtcgg cgctgcttct gggccaatac cctctattgg ccgatcggtt ttgtgccgac
      301 gagggcgtcg gcactacttt gggtgtcggc actacttggg gtgtcggcgc tacttggggt
      361 gtcggcgcta cttgagggtc gcttcgttat tcagttcgta gagttcctgg acctggttct
      421 gcgggtcgag gcggatgcgg gcgttagcaa gcttgtccgg gtcgacgttc accacaatct
      481 ctcgaaccga gggctcgaag ctcttgatgg ccggtagccc atcgactttg gcgtgagcaa
      541 gctgcgtcca ggtgtcgccg ttcccggcct gggccacgac gtcgaacggt ccggccgccg
      601 ctccgccgat gttgtgaacc cggacaacga ccttgccgtc cttcagctga atgtcccgcg
      661 acgcgatggc caagtccggc agcatcgcgg gcctttcgat cgccttgcgc agtcgcagat
      721 cgacgaccca ctccttgtgc gaggggattt ccacccagat gggcgtgccg cggtggagat
      781 gctccacctc ggtcggttcg gtccaggtgt agcgtttctg gaaatcgtat tcgccgggca
      841 cgggctcgcc ggcgttgagc acgtagacgc cgggcaccag ccgccagggt ttgagcccga
      901 cgcgcatcgg ctcggcgttg aagttgtaga gcatgatccg cagacgagcg ggtgtggtct
      961 cggtcactac cgaggcgaag ttgaggtccg gtgtgtcgta ggttaccgag atggtcggcg
     1021 gtttgctgtc gcggaagctg cagacggccc cggtgtaggc cccgaagacc tcggccgacc
     1081 ctgtcaaccc ggcccgatcg gtttgcagga tctcgctggt gagctgcgtc cagttatgcc
     1141 gcaaggagtt ggccgtcttc tcgaacgtcg tgacgtaccg ggccaagtcg tagtcggtca
     1201 tgtagcgttg cgtcggggtg gccattcgca ggatgtactc gtcgtagacc cgctcgccgg
     1261 tcaggaggcg gtaagcgctg agcacgtcgg ccgaagccat gtgcgaccac tgggccatga
     1321 ggttctcgat atgatcgggc ggcagcttgg ggtcgccctt gtggagcgga ccggcggtgg
     1381 ccatgtgcat catcgtctgc accgggtcga ggaacttacg gtcgttggta agcagatagg
     1441 ccgtcgtgaa gctctcgtag atcatgaggg gcaggccggg gaagccgtac gaattgcgct
     1501 gctcgtcata ccagggtttt ccgtcgggcg gggcgatgcc tcccgaggga aaccacaagg
     1561 tggacggagg gaagccggcg ggtttggggc cgatctgccg catggtggcg tcgcgccagg
     1621 tgtcgcacca ctgcacaaac gcgtcgcggc cttcgggcac cccataccag ccgagataga
     1681 tgaaatgctt ggttgcccgc gcgtggtagc cgctgtcgcc gccgttgccc aggccgcgtt
     1741 tgacaccgtc ggtgccgaag tcccgcgaca tgaactggag gaaaccgcgc tcgttgaggt
     1801 ccatgaactt ctcgcgaacg gtcttggcgg atcgcaggtt gcgttcgagc cacaacggat
     1861 cgccgtagcg gaggacgatc atggtgggca gggaatcgca ggacggctcg gcggagtgct
     1921 cgacgtcgcc ggggaacgga tcgtagccgt ctttgagaac gtggtcccag acgccctggg
     1981 ccagtcgctc gataccggcg atgaccggct cggccgcgct ggtgatgcag gcgatgggca
     2041 cccaaccgcg aaggatctcg acgtcgtcgc cccagccgcc gccgagttga ccgtccggaa
     2101 gctgtcgttc gctgaaccac cagcgtagga cggcctgttg gcgggccagt gattcctgca
     2161 agtggcgagc ccaggccggc ccgtccgact cggggcggat cagttcctcg ccccagggga
     2221 tcttctcgcc ggtccactcg cgcagggcct ggtgttcggg ccagatcttc ttgagttcgg
     2281 cgaactcatt gatcgctgcc tgcatgccct ctcgatgggc accctcgatg gcatgggcgt
     2341 agttgaagcg attctcgaac aggcgggcct tgtagtagaa cgggttgttg tccgggccgc
     2401 agccgatcag tcgacgggtg gcccgatgat cgcgcctgta gacgttgaag tcggcgaacc
     2461 acttggcggc cagggcgaaa tccttggctt tggtgtcttc gggggccctg gcggcgagct
     2521 ccgcggcgac tctggcgacc tgtgcctgat gcgccggttt ttccagaaac gccaggtcgc
     2581 ccaccacgta ggccagagcg gccgcgctct cgagtgtggc aggtgagacg gccgagagcg
     2641 tagcgaaggc ctcctggtac tttcgctcgt tgtacagcct gatcccctgg gtcacgtttg
     2701 gtgtcagggc ggccggcccg ctgtaactca gttgacgatc tttccaggtg aatcccagcg
     2761 ccggcatggc tcgcagcacc accggtccgc cgggcgcggg tgttgtccgc tcgaagacct
     2821 tcacgtaatc gacatacatg ccgttccagg tggtcagcgc gacgccgtcg aactccggcc
     2881 ctccgagcgg atcgtcgagc ctggcctcga ggagcttgtg gtcgttgcag tagaggatca
     2941 gcatcggtcc ttccttgacc gcgacgatct tgtatttctt gccgtgttcg atcgggaatg
     3001 ggggcttgtt gtcccgaacg gtcacacccg cgcccatgat ctggttgaag gtgttgttgc
     3061 cgccgccgaa agccaggagg aaggtgttgg ccaaggtgac ggccaggtcg cagggcggct
     3121 ggtcggggtc ggcctcagcc aggaactcca ccttcacgtc gcccgggaat ccgcggcgga
     3181 gcctggcgtc cgcccgcccc ttgagaaaca ggcggccgtg gacgatgctc acgttcttgg
     3241 cttcgaccca gtccggtcca agctcggccc gctggaagcg gtcctcgaac acgagcttcc
     3301 atggcccgtt atcggccgca ttggcgatcg ctgcgaaaac cagtacacac gcgcaaagcc
     3361 atttctgcat gacacgactc ctcagtgagc atgcacggcc acccggccat gatgaaagag
     3421 agcctctccg caaagcccgt ccgaaagcag gttgtcatgg atcgcagggg ttgtgtcaag
     3481 agtcgacgga attgagggga tgaggcccgc tgccggtgcg acggtgaacg tgttgcgacc
     3541 tccgtttggc tcgcgcacaa aggcctgtcg tgccgcgttg agcccccgct tgcgggaggc
     3601 tcaggctcct tgcaaaacag cctcaatccg ttcatcatgc cgcacaatcg cagtcacccg
     3661 atctctgttg aggagttcat gatggccgga accctgaacg ccccgaccga acccgttttg
     3721 gcacgtcgca ggccccgcga gaactggggt tctcgtctgg gagtgattct ggcggtggcg
     3781 ggctcggcgg tcgggctggg caatttcctg agatttcccg gcctggcggc gacgaacggc
     3841 ggcgggatct tcatgatccc gtacttcatc tcgctgctcg ttctaggcat ccccatctgt
     3901 tgggtcgaat ggaccatcgg ccgttacggc ggagcccgcg gcttcaacag cgctccgggc
     3961 attttctccg ctctctggcc gagccgggtg agcaagtact tcggagcgtt ggcgctgatc
     4021 attcccgtcg gcatctacat gtactacgtc tacatcgagg cgtggtgtct ggcctatgcg
     4081 tgggactacg tatcgggcac catgactcgt tacggcgcag atgccggcac ggacgtgacc
     4141 gcggcggttg ccgcgtaccg gacgcacttc accgagtttg tcggcatggg cgaaaacggc
     4201 ttgctggttt acggatccct gcaaaaaggc gtcgtttttc tgctgatcgt gttcgtcatc
     4261 aactttgtcc tcatctaccg cggtctgacc aagggcattg aagccttctg caagtacgcc
     4321 atgccgttgt tggccgtctt tgcgctgatc gttctggttc gcgtgctgac cctgggcgct
     4381 cccgattcct cccgccctga gatcaacgtc aataatgccc tcggtttcat gtggaacccc
     4441 aagaccggtg acgaaggttt tctggcggcc ctggccaacc cggaactgtg gctcagcgca
     4501 tcgggccaga tctttttcag cctgtcggtg ggcttcggca tcgtgctgaa ctatgccagt
     4561 tacctcaagg cggacgacga cgtggtcctc tcgggcttga ccgccacttc gaccaacgag
     4621 ttctgcgaag tatgtcttgg cggactgatt acggtgcccg tggccttcct tttccttgga
     4681 gaagccaacc tgaccaagga cgtgctcggt tcgacgttca gcctgggctt catgtccctt
     4741 ccggcggtgt ttgcacggat gccggcgggc agctttttcg gggcggtgtg gttcttcatg
     4801 ctctttatcg cggcgatcac cagctctctg agcatgcttc agccggcgat cgcgttcttt
     4861 gaagaaggct tcggcatggg ccgacggctg tcggtgacct gcctggggct gctgacggcc
     4921 attggcagtc tgctcgtcgt ctacttttcg aagaacctgc tggccacgga cacgctggat
     4981 ttctggattg gaacggtttg catcttcatg ctcgccacgg tggaagtgat ccttgtcggg
     5041 tgggttttcg gcgtgggccg tgcccgcgag gaggtcaaac ggggggccgc cctggaagtg
     5101 cctcgagtct tctggttcat catcaagtac gtctcgccga cttacctgct ggtcatcttc
     5161 gggttctggt gctacaagaa cataccggcc aagatcgacg aagtccgggc gatgagtccc
     5221 gaagatcgcg gcacggtgct cctcatgctg gccttcatcg gggtgctgct gatcttcttc
     5281 gggatgctgg tcaacctggc cgacaagcgg tggcatttga tcggcaaact gccgctggag
     5341 cccgcgcgag gtttgccgtt ggccgccgcg gatgaagaag ggagaacgcg ctcatgacct
     5401 gggccggatg gctgctgatg acgctttcct gcggggtggt ggtctcgctg atggttttct
     5461 gctaccgacg cgtgctgcga acgcccagac ccgaggagca catgcacgct cctctggaga
     5521 tcgacactcg cgacgcggac acttgataag ggttcggggt tcagggttcc ctgcttcgct
     5581 cgatgagctt cgcagggcaa acggggttca gcggagcggg gctcggcgca ggctgcattg
     5641 atcgatgcgc ttggtacttg cggagcgaca tggtgaaggc gtccctgagt ttgcgcgtcc
     5701 gctcgttctc gtgagggatg aacccaaagc gctcttcgtc tttgggcaac tgcattctgg
     5761 cgattcgttt ccaacccttg ttcatcccgt ggatgaagag cgagccggtc aggcttctgg
     5821 tcgccagcag cgtccttaac gtgcgtcgcc agacccaggg ggtattcgcg gcggcgtgtc
     5881 gcagttcgta gatggtctca tccagctttc gggcggtcat tttgcgcggg tggtacacgg
     5941 tctcgacgaa ggtatacctt tcccagtcct ccgggtagtt ggtagcgaag atccggccct
     6001 cggacatcca cctttgatac agcctggtgc cgggcagagg cgtgaggttg gtgatctgga
     6061 tggtatcgat ccccagcttg accgactgaa tggccgtctc ggcgacggtg ttctcatcgt
     6121 cctggtcggc accgatgatg aaggccccga agacgggaat ccccgcgcgg tggaatccgt
     6181 cgacgagttc cttatagcgt gacagattca tgcggttgat acccttgtgg taatcccgca
     6241 ggctctccgg gttgaacgtc tcgaagccga tcagcattcc gcggcaaccg gccctgtaag
     6301 ccaggtccag tccctccgca tcggcgccca tattgatcgt ggtctgactg aaccacaggc
     6361 gtttcttgcc ccgcttgatg atctggcgaa gcagctcctt ggcccactcg gcgtgcttgg
     6421 gcccgacacc gaagaagttg tcgtcggtca cgaagatgaa ccgcttgatg gtcgagttcc
     6481 actcgtcgat gatatcgtcg atcggtcgcc gccggatctc cgggccgctg aataacgtga
     6541 cgctgcagta gtcgcatccg accgggcagc ctcgtgaagt ctgaatcgcg gcgacggtgt
     6601 atgaaccatt gacggcctga aggtgctgag ctccacggcc gatgcccggg gtactgatgg
     6661 aggccggccg cccctggtat cggggtttga gccgaccggc cgcggcgtcg gacagaatct
     6721 ccgtccaaat ctcatcgcac tccccgatcg cgatggaatc gacatgtcct gccacctcgt
     6781 cgggcaccgc ccaaacgtgg ggtccaccca taatggttgg aatgccctcg cggcgagcct
     6841 gggctgcgat ttcgtaggcc cgcgacgcgc cgctggagta gctggtgatg cccaccagat
     6901 cgtatcggcc gcggcggacc aatcgctcgg tggcttgggg tccgaagatc tcgtcaatga
     6961 tgtccacttg atggtccgag ggagtcaact gggcaaggac ctggaggccg agctgcggga
     7021 ttctcccgcc tatcgtgtgg tagccctggc atcggcgggc aacggggtta atcaatgcta
     7081 ttctcatgtg aggcctttca cgacaaccaa gcccgatccg gcgacaaacc acggtcgtgc
     7141 aaagtcatgt tgtttgttgc tgtcaggcgg tgagacacac gaagctcaac acgacgggtg
     7201 gcaagcaagc ctacagcccg ggatcgccgg tcgccggtgt gggttcttgg atgcgaatcc
     7261 ggtcctgatc cgcgctcata tccgtccggc attaagggat gacgataacc gcaaccggcg
     7321 ggtgaatcaa ccctcgccat gaacccgagg tcaatggggt ggtatggcgc aaaaaacgag
     7381 ccccgcaatc gggcgcgggg ctcgctagtc atatgtcgtg gctgccgtaa ggcagctgcg
     7441 tgggcgttcc ctacggcagc tcacctctgc aatctgtcaa gggaccacct cctttcggga
     7501 agaaacacct cgggtcattc gctgcggtcc gcacaagcac cggcacaaat gtcgccgagc
     7561 ttcatcccac ctgccgatcg ccaccggcag gtccacaggt tgtgatcatt ccagaaaccg
     7621 gttgaatctc ctccgaccct cccggcgaca gactgacgca gtccgtttcc ggaaaggtct
     7681 gacacctgcc tctatctatc tatacgtcag gatggctgtt tcggcaaaga aaaatcactg
     7741 aaaaagggag taatcgcgtt tgttgggtcg cggacggggc aggtggttac ccgatttcgg
     7801 agcgcctgca gttaccggtc cggtctgaag gctcgcgggc tgccggcctt cacaacagat
     7861 gtgccgaata gggacaaacc tcgcgggttg ctcaagaatc gccccccgct gcaacctggc
     7921 cattggttat cggtatcaga gggcggtcgg ggtctgcgca tatgctggcg gtgcttgcgc
     7981 ctcgatgggc gggcgcgcgg tcctgggctt tcggttcgac tggcgcgcac ggaaggttga
     8041 cggcaatatc ttagaagtat ggtcgcgtgg gcgagggctt ttcgatagat attatgaggg
     8101 ctgcccgccg tggattgcgg gtcagggacg tcgatcgggg ttcgggagga ggttttatga
     8161 tcgacggacg cggcaccccc accggcttgg gctccccgcc cgctgcggac atcggggttc
     8221 gggaggaggt ttaatgaccg accgactgtt tcccatcttt gaggatcacc gcagagtggg
     8281 gctgacggtt gtcttggcct tctgttggct gacaggctgc tcatcggtgt tcccgaccaa
     8341 ggtggagccg ctgctggatc ccgagtctct gcggccgagt ccccaggttt ggcagttgag
     8401 cgatccgctg gatccatcca agccggccac tcagccgatg gcaacgcgtc cggcgggctt
     8461 ggcggccacc cgtccggctg ctggaccgag cacccgtccg gcgaccggtg agttgcccag
     8521 ccccccttac cggatcaatg ccgacagcat cccgaggctg gtttatcaca agtatccgct
     8581 ggttacttcg gccagagagc agatgatcgc cgcccaacac ggcctgcagg agttcaaggc
     8641 caacctgagc cgttttgaac ccttcaccaa cgtgtcgggt atggcctttc agtatcccga
     8701 gcgacgcgat gccagcggat tgtcgggcga ggtcaccggc gggatcgaga aggagacttt
     8761 tgacggcgcc atctacaggg ttgagggtgg tgctcgcggc gagcgaatca aatacggcga
     8821 agtgggagaa gggcaggcgt cggaggatag cggcgagggc ggtctggtga gaggccgtgt
     8881 cgaggttccg ttcattggtt cccgaaagcg gcaggaccga acgatcaacc aggcctacca
     8941 ggaatcgacc gcccgggcgg cggtgctcaa ctatctgagc tactatcgct cttacgccct
     9001 taccgcgatg aactactatg ccggcgcctt gctctatctg ggctatgcac gggcctacga
     9061 acaccagatc gaggcccttg aggatctgct caaggagccc gggctcaccc ctcaggatca
     9121 gcaagtcatt ctgacctcga tcggcagcag ccggatcgtc cgtgactcct acaaggccag
     9181 ctatcagtcg tacctgttgt ggactctgga gtatctgggt attcgcccgg gcgaggagta
     9241 ttatctcgag gagccgccgg tgagcgaggg gagcatctac tatgaccgca cccggaccga
     9301 cgaacaaaga caggagcttc tcaccgaggc gtacgagagc aatccccggt ttcgggtgct
     9361 gaacgacgcc atccgggacg ctgaactgaa gcggtcgcag gccattctcg ggaagaacga
     9421 catcaccgcc tttgtcgagg ggacacagca cgccttcgga gcggagacct ttgacgatcg
     9481 agtcggcggg tgggaattgc ggggcggcgt gtccttccgg atgaatgatc ctcgagtcct
     9541 caacgcttcg atcagaaagg ccgaggctga gatccggtcc taccaggccc agatcgaggc
     9601 tgaggaactg agtgttcagc agcagattgc ggtgcaatcg gccacgctga ccacctacgt
     9661 tgaatccaag ccgcagattc tcaagaacat cgacgaggcc aggagcgagt ttgaggctcg
     9721 tcgcaaggcg tactttgcgg gccagtcgac gctactgaga atcgatgacg ttctgagttc
     9781 gctccagagc atcacaacgg cagaagtgcg cctcgtcagc aacaggtatt acaccgcttt
     9841 ggcggataac atgctcatgt ccgcgtcggg acagctttac cagatggccg gcgtgaagct
     9901 gacggaggac gccggcacgg tggggcttgc ggagaagaag tagtgaaagc agccacgggg
     9961 tgccgtgacc aggcgcggac aggagtccgc gtggcgagaa gccgcccccc aggcaaaccc
    10021 atgaacacac agggacagga tgccaagatc ggccccggcg agacaggctt attacgcgcg
    10081 gcgacgaagc atgctgctga gcctctgtgc gcttgccgtg ggggcgtgtt cgaccgttga
    10141 ccggcgcgag gctccgccac cttcgggcac cgggggagtc aaccgtcgga cccaattatg
    10201 gcggacgccc cgaaggcaac caagccgcag agtggcgtct cgcccggcag ggtcacgacc
    10261 ggcgacgagc caagcggtcg gcacgcggcc cgcggcaacc cggccggccc tgcccccgct
    10321 ggcctcggtt cgccccgtcg ccatgacccg gccggcctcg tcgcaacctg ccgagactcg
    10381 cgaacctctt gccaagccct atcgcattaa cactgccaac gttgttcgga tggcttatct
    10441 gatcagcccc ctggtgattg ccagtcgcga ggacatgatg gccgcgaggc acggcctgga
    10501 agaattccgc gccaatctca gtcgccttga gccttatatt ggggttgacg gaggagccct
    10561 tgaatatccg caacggcggg gggtcaacgg ctacgacagc gagctggttg ccggtatcca
    10621 gcaggagacc tttgacggat ccgtgtttcg cgtcgaaggc ggggtgaacg gctcgcgtta
    10681 cagctacgac cggacgcaaa gcgacgcggc caagacggaa tccggcagca gcggagtctt
    10741 gcgagcccgg gtcgagattc ctttcatcgg ttcccgcaag cgtcaggatc gaaccatcaa
    10801 gcaggcctac caggagtcca gcgcccggca ggcgatgctt gaatacctca gagactaccg
    10861 aacctatgtc aacaacgcgc ttgccgaata tcgccagacg attctttacc tgaaccagat
    10921 gagggcgtat gaggatcaca tcgccgcgat cgaagccctc tgggacctgc ccgagctgac
    10981 ggacgacgag cgcacgcgct tgcagacggc tttgggagat gccaaagttc accacagcgg
    11041 cctgcaggcc agctactggt cctcgctgct ttcgttgttg gaaactcttg gtattcaacc
    11101 gggcgaaaag tataccgtcg aagagcccaa ggaattgtcc ctacagttcc tggacgaggt
    11161 ctcgacgccc gaaggccgac agcgactgct ggccaaggcg tttgagaaca atccccggtt
    11221 tgcggtcctg gaggatgcga ttcgcaatgc cgaactgaag cgttcccagg ctgttttggg
    11281 aactcatgac atgaccgcct attttgaggg ggcgcactac ccgtttgccc cgccggagtt
    11341 tgacgaccgg gttcacgggt ggcttctgac ggcggggatt accgtgcgga tcaacgaccg
    11401 gcgcgtcctc acggagtcgc ggaagaaggc ggaggccgaa atccgggctt accgggctca
    11461 gatcgccgcc gaagaaaacg atattcgcga tcagatcacg attcgcgcgg atcggctcct
    11521 gacctactcc aaatcccgct cggaggcgat cgaaaacgtc gagcaggcgc gggcgcagtt
    11581 ccaggaacga tgcgaggcct acttctggcg aggcgagtcc gggatgaata tcgacgacgt
    11641 cctcaggtcg ctgtcggtcc tgacaaacgc ccaagtgcag cttgccagca acttcaatca
    11701 gatcattgat gccgaacacg atctgttggt tgcgaccgga gaagtctatc gcttggtggg
    11761 catccgaatt gagaaggaag actccggcac cgtgttgcac gaggcgctgc tctcacagcc
    11821 gtctgttacc tcatcacaac ccgagtgact gattttactc aggcagattc atttgcaggc
    11881 gctcatttga caagccggat ccgcaagcaa cgccaaccct gctctgcagg acaaagtcgc
    11941 aacgcggcgt gagggcgagc gtcactgctt gccggtccta cggcggttga cgagatacgg
    12001 cttgccggtg cggcccctgg aatgctcaca gggacggacg ctgaacgttg acatccgctt
    12061 atcgctccgc gcagctcgga tcggccggca cattttcccc gctgcagcat cgctggaaga
    12121 aggcgaagtc ggtctgatcg gcatcaccat cctgatcaaa atcgattctg tcgcagccag
    12181 gcgacaggga gacgcccggc gaagtggcgc agttttcaaa cagatcggcg tcagcgccgt
    12241 ccacgtcggc gtcgcagtcg agatcggccg gggtcagccc ggtgcgcttc gctttgattt
    12301 cgaacgagac atcctgcccc tcgcaaatcc ggaagaggta ttggccaccc ggtgacagat
    12361 cgacgttttg ctgaccggtc acatcgacca gcgtgtagct gtaactaccg atgttgctca
    12421 ggtcccaggt catcgttgcc tgttcgcaat gcccggcccc gaggccggcc aagcttgcgg
    12481 tcgcttccca gacctcgtcg ctgccgcagg gaggctctcg gtaatcccga attcgcggtc
    12541 cttggaaatc gggcgtggtg gtaagccgga gttcggccag tccagccggg gccgcggggg
    12601 gagccatcag gtcgagcgca tcgagttcat ccgatgctgc cggatcgacg gctgctcgga
    12661 agctccatgt ggcagtcgcc tggccggtta tctgaatgcc cagcgaggag ccggtttcgg
    12721 cgccggcgtc cagaatgccg gcagcgccgc cttccggggg aaccaccaga atcagtccat
    12781 gcctgaaagc ccggagccag tagccctgac acgggcggag gtaggcgtcg tctcccgttt
    12841 cgccgggtat caccagccgg tagccggaaa ccggttcatg ataaagcagg gtcgactgga
    12901 tccagcctgt ctgtccggcc tcggtgacgg tttttgtctg cgagccgtcg gagacggaac
    12961 aggccgccca gaggaccggc acatccgtgg ggcatccaat cagattccac ccgtcggtca
    13021 gtccgatgag cacattctgc tttgccgctt tcccgcgcac gctgaacgtg gaggcctggt
    13081 caaggcgcag ccaatatcct tctccccggg caagcgtggt catctcgtcg ggatatgcct
    13141 gataggcgtc tgcccggtac cggagcagat gcccgggcaa ggcattgccg gcctgctcaa
    13201 gggccgccag caccgctgtc acatttgggt tgtcggagtc gagcgggatc gaaatgagat
    13261 tccagccgcc ctggagccag ggcggtccgc accgaaaccg ttcggacatg ggcagtgccg
    13321 gcacgctagc ggcgccgccg ctgtccggct gatggaacat ggcgattgcg gcgaagcgtc
    13381 cgccgccgga cgcgcgcatc tcgaacgagg gcacaggctg acccggcaat ggcgggatca
    13441 acggcaggat ttcggtcatg tgtggcgcca attccatggg agcgccgccc tgcggcgtgc
    13501 cgtccaggcc gtacaaccgt aagtcgatgg ttacgggttc ctcctccggg ttgtgcatga
    13561 cgagcaggcc gtcctggagg agcggcacgc ccggcgacgg gatcctcaag gacgaagcgg
    13621 ggcggaccat agattcggcc atcgtcgtag cctcgttggt cgtgcggacg catgaaccga
    13681 tgatgccttc gccctccaga acgcgcaccc ggactgactt agccaaggca ggcggtccga
    13741 gatgatccgc gtcgaaagtc attgtggcct tgggggcaat cagctcgggc aggctgccaa
    13801 gaaaggcacc gcctatgtgg taccattcca ttaccaccaa ggcggccgag tccgtcggat
    13861 tgtgaaggat taccattgtc tgccagtcgg cctccgaggt cacgtagggg gcgatcagct
    13921 cggcggcgac cgaggtctga gcggtggtct cgaaggcgtc gtaggtaagg tcctcggcca
    13981 aggcccaccc gagaagatgc ccttgcgaga gctccgcgtg catggccgcc gcacccgtga
    14041 gccatgactt gggcacctcg aatcgctggt aggcgttcac cgtggcctgc ttggtgtgaa
    14101 gcagcctacc gctcatatcg tagaagcgca tggtcaacag agccgtcgac tcaggcgggt
    14161 cgtacaccca agccgtggta tagcgccagc ccaagccttc ccctccgaac atcggcccgg
    14221 aaggcaactg gaacgccagg gtggcgatcc aggcttgagg ccccggctga ttctggccag
    14281 gtgctgcggc ggttccgcgc ctgccaggcg cggcccaggt ctcatacccg ggaatcatct
    14341 ggtccatggc gatcgtgccg gccagggcgg tcagatcatc gtccaacgtg tcctcgtcga
    14401 tcacgatcac gccgtgggga ggcggcgcca tggttgactg ccagcccagc aatgtgccat
    14461 ccagatcgta cgcccaggtc gagccgtcag gcagggcatc gtcggtcggg ttctggatgg
    14521 tgatcgtgcc cgtggcctga atcaatgact cccatgtgga cttggcctca tcgcgtttgt
    14581 cggtgacctc ataagttccg gaatcggagc ttgactggta ttcgatcacg tagcagccgg
    14641 gcgggtcggc cggatcgaac tgccccgaga ggaacgtggc gtagacataa acactgaccc
    14701 gtgaatccca accgccctgc ggctgggcga aggtgtgctc gaacccgccg ttgccggtct
    14761 gaacggtggc ctcatagtgg ttgccggcgt gatcgagctc gaccttgact ttataatggt
    14821 tgtccatggc aatccagttg ccgccttcct cgggttttct tttcaccgtt cccttaacct
    14881 tgatgtcgct ccacttgtag acggaagaga ccggggaggt aatgtcggtc tgggtgggga
    14941 tcttccagga agtgtcccga aacctgaaag tcatcgtgtt tgactcgcta tccagttgtt
    15001 gcccgtccgt attcctgacc tgggcgtaga tagtcaagtc gtagtctttg tagtacctgt
    15061 tcccaccggt cgaaagcttc tgagagagcg ttgttccgta gtagtcgcct gagcatccct
    15121 gcgtggtgtg ggagatctcg gcggcctcgt tctcgccgcc cgagaagcgc attttgtatt
    15181 tgtagttgtt ttgccagtca ttatcgttga agtagcactg gacatagacc cataaggtcg
    15241 gtccatcgtt ccagacgtcc gtatcctgat tggcgtccca ctttgcttct gcgtcggcgt
    15301 tctggcagca gaacttgttg acgtaaagct cccagtcacc ttgggccaga gctgcgggca
    15361 aaagaacata cacgaagatc agaatccggc tcgacatggc atacctcttt cctgcggcgg
    15421 tgacggccgg cgcggcccgc ttagaaaaga acacaaatgc gaaagccgga cggtgccgat
    15481 cagcccgccc gcgtatggac tcgacgcgtg gaaatgaacc agacttcagg ttttatcata
    15541 ctgcgaggct agcggcgaag caagaccaat aacagggttg aaaagggatc atctgggttc
    15601 gggtttggtc agtccagtcg cgagaccagg atacaggcgt tgtggccgcc gaagccgaat
    15661 gagttgctca tggccgttcg gattcgggcc tcgcgggcga cattgggcac ataatccagg
    15721 tcgcactgcg ggtcggggtt atcgagattg atcgtagggg gcagaatgcc gaggtgaatg
    15781 gccagcacgg cggccaccag ctcgacgccg ccgctggcgc ccagaagatg acctgtcgcc
    15841 gacttggtgc tgctgatcgc caccttgcga ctttgttcgc cgaggacggt cttgaccgcc
    15901 agggtctcgg cggtgtcgcc gagcacggtg cccgtgccgt gggcgttgat gtagtcgagc
    15961 tcgtcgggat tgatgccgga gtcgttgagg cagtcgcgca tggcgacggc ggcgtaatcg
    16021 ccggtctcgt tcggctgggt caagtgtgat ccgtccgcgc tgcacccgaa gcccctgacc
    16081 tcggcgtaga ttctcgcccc tcgctgcctg gcgtgttcgt actcttcgaa gataaggatg
    16141 ccggccccct cggccatcac gaatccgtcg cgatccctgt cgaaggggcg gctggcccgg
    16201 gtgggttcgt cgttgcgggt gctgagagcc ttcatggcat tgaaggcgct gaggcccagg
    16261 gtggtgacgg cggcttcggt tcctccggtg aagatgacgt cgcattcgcc gcgctgaatc
    16321 agtcggaggg cgtctcccat ggcattggtc gcggaggcgc atgccgttgc gaccgccgtg
    16381 ctggggccgc gggttccgaa gcggatggag atattcccgc tggccgcatt gaccatcagc
    16441 tttggaatgg tgaaggccga gactttggag ggtcctttgt ccatgaggcg ccgatgctgg
    16501 tcctcaagct cctggatgcc gccgatgccc gagccggtga tcacgcccac gcggtgcggg
    16561 ttgattctgg agaagtcgag tccgctgtct tgggccgcat caatggccgc ggcgacggcg
    16621 aactgggcga agcggtccat gcgtttggcg gccttgggtt cgatccactg ttccgggtta
    16681 aaggaattgc actcgcccgc gaaatgggtg tcgaaagcgc tcgcgtcgaa ccgttgaata
    16741 ggcccgatac cgctgcgtcc cgagatcagt ccgtcccaat actcgcggac ggaacagccg
    16801 agaggcgtta ttacgcccat gcctgtaatc acaacccgcc gtttcgccat gcttagcatt
    16861 ccaggaaccg cgccaaggtc agcttgaggt cgccgcccaa ggttccttcg agacccggag
    16921 atcggggcgg ccgcattggg gtttgtgccg tcaccgactg ctggggcacg agttcgcgga
    16981 agggcgccga ctgccggagc ggccggggcg cggacccgtc ccaccgactc actactgctg
    17041 ctgctgttgc tgaccctggt gtgcgacgat gtagtcgatc gcctggccca cggtcttgat
    17101 cttctcggcc tcctcatccg gaatctgcgt ctcgaagctg tcctccagct ccatgaccaa
    17161 ctcaacgatg tccagcgagt cggcgttcag atcgttgatg aaagagacct ctcgggtaat
    17221 ctcggccttg ttgattccca tctgttcact tacgatcgat atgaccttgt cttcgatttc
    17281 ctgaaccgtc actccgggat cctccgtagc ttatcagatc cactgtcgca actacggcct
    17341 cttgcaggcc actccgtaac cttacgcggc cctctgcggt ttttcgccag gggccattag
    17401 tagactacat attcagcccg ccgtccacca ccaagacctg tccggtaata tacttggcct
    17461 ggggggacgc caggaagatg acccctgcgg ccacgtcggc cggctcgccc atcttctggc
    17521 aggggatcaa ctgcttgacc atgtccttga ccttgtcggg cagtaccttg gtcatctcgg
    17581 tatcaatgaa gccgggggcc accgcgttgg cggtgatatt gcgccgggcc agctctttgg
    17641 cgaccgtctt ggtgaaaccg atgactccgg ccttgctggc cgcgtagttg gcttggccgg
    17701 cgtttcccat catgcctgaa acgctggcga tgttgacgat ccggccgtaa cgggcctgca
    17761 tcatatgacg acaaaccgca cgggtcatca ggaacacgga acgcagattg acgttgatga
    17821 cggtgtcgaa ctgctcgtct tccatgttca gcaggagacc gtcccgggtg atgccggcgt
    17881 tgttgaccag gatgctgatc ttgccgaact gctggatcac ttcctcgacg aagctctcca
    17941 ccgccgcccg gtcggtcacg ttgagcgact tgggcacgat cttgccgggc aatccgttgg
    18001 ttttggcctc gtcggcaacc gctttcgtgc cctcgaaatt cagatcggca gccaagaccg
    18061 tggccccctg ctgagcgagc ccgaggcaaa tggcccgccc gatgcctcgt gctccgccgg
    18121 tcacaaccgc cacttgttcg gtccacgaca ttccgatgtc ctcccgctgc gttgtccgca
    18181 ctgtcacccc gaatcgatcg cggccgcgtg ggggctctgc accgcccccc gccggttcga
    18241 agccggcctt aagcggcttt cagagccaga tctgccgcag tgctgacgtt gatgatcggg
    18301 gttcgcctcg atattttttt catcagtccc gtcagcacgc ggcccggccc aatctcgata
    18361 aactgctgca cgccctccgc caaaagcttt tgcatggact cctgccagcg caccggctgg
    18421 gtcaactgct ccgtcaaccg ccgacgaatg gtatccgggt ccccatgggg ttggcaattc
    18481 acattggcga tgaccggcac ccgcggggtc tcgaacgggg tgaccgcgag catctcggcc
    18541 aggccgtcgg cagccggctt catcagcggc gagtgaaaag ccccggcgac cttgagcggt
    18601 accgcccgcc cgccggcctg ctcgatcagc aaagccgcct tttcgcaagc tgccttggct
    18661 cccgagagca cgatctggcc cggcgagttg aagttcgcag gttgaatcac acccgcgtcg
    18721 gaggcttggc gacagatcgc ctccacttgg tcgggatcga gacccatggc ggagaccatg
    18781 ccgctgggaa ccgcagttgc cgcttcctgc atgagctcgc ccctgcgttt gaccagccgg
    18841 agggcgtcct cgaacgccac ggccccggca gcgaacagag cggtgtactc cccgagactc
    18901 aggccggcca tggctgcgac gggctcggtc aagaccgccc gctcctgcaa cgcctgccag
    18961 atggcggcgc tggtcgtgaa gattgccggc tgttgaaggt cggtttgctc cagccgctcg
    19021 gcgggcccct cgaagcacaa ggcgcgaaga tcgtagccga gcacctggtt ggcccgctcg
    19081 aagacggcgg ccgcggcggg agacgcctgg gcgatgtccc tgcccatgcc caccgattga
    19141 gcaccctgcc ccgtgaataa aacggccgtc ttggccatgc ccaggaaacc tcacaacctg
    19201 atcagagccg agccccacgt caggcccgcg ccgaacccag ccatcagcac ccagtctcca
    19261 gtcttgacct tccctgcccg ccatgcctcc tcaagagcca tcgggatcga ggcggcggag
    19321 gtgttgccgt agcgatcgat gttcaccgcg atcttgtcca ttggaagtcc catcttctgt
    19381 gccgccgact cgatgatcct caagtttgac tgatgcggga cgacgtaggc caagtcttcg
    19441 acccgcaggc ctgcgcgtcg cgtggtctca atcaggatct cctgcatctt ggtgacggcg
    19501 aacttgtaga cctcgcggcc ccgcatcttc atgtagtgcc ggcgatcctc gaccgtcttg
    19561 gcggtggcgg gcattgccga gccgccggcc gggaccatga tgagccttgc tccgctgccg
    19621 tcggctccga gcatgtgctc atacaatccg ctgagctcat tgtcgggcgg gccgatgatt
    19681 gcggcgcctg ctccatcggc cagaatcacg caggttgagc gatcctggaa atcggttatc
    19741 gccgacattt tctcggagcc gaccaccaaa acatggcgat accccccgat ttgcatcaaa
    19801 cctgccccgg ccacgaacgc atacacgaaa ccggcgcagg cggcggcgat atcaaacgct
    19861 ggaacgtgcc gacaccccaa agcgtgctgg aggaagcagg cggtcgccgg caacggatac
    19921 gcgggcgtgg aggtcgcaaa gacgatcagg tcgatgtccg cgggggtcag gccggcgtcg
    19981 tcgactgcgc gcttcgcggc ggtggtcgcc agcgtcagcg aatcctcgtg gtcgtcggcg
    20041 aatcgccgtt cgcggatgcc cgtccgcgtg cgaatccact cgtcggacgt gtcgagaatc
    20101 ctggcaaagt cgtcatttgt catcacgcga tccgggaggg cgctgcccat gccccgaatc
    20161 gccagcggaa ttcgttcgcc catcagtact tcacttatcg ctaagccgct gtgcggcagg
    20221 ttcttcgacc agttcagcgg caataatcga gttcaggtcg ttctcataat actccgccgc
    20281 cactcggacc gcgtttcgaa tggcaacgtg atcgctggag ccgtggcaga taatgcaggc
    20341 tccgtcgacg ccgagcaagg gtgcgccacc gtactcggcg tagtcatggt tggcccagac
    20401 gcgcttgacc acgggttcga aatgcctggc caattcggga ctggccaggg cgatttcctt
    20461 cgagatggtc ttgaacaggc cttcggccaa cccctcgatg agcttgaggg cgatgttgcc
    20521 cacaaacccg tcgcagatga cgacgtcgca gccgccgcgg aacaaatccc ggccctcgac
    20581 gttacccacg aagttcacct gcgggcacga atcgagcaat tcgtgagtct gtttgacgag
    20641 agacgttccc ttgacgtcct cttggccgac ggacagcaat gccactcgcg ggcgggtgat
    20701 tttgagcacg tgtttggcgt atgccccggc catgatggcg tactgcacga ggtggtgcgc
    20761 tttgggagtc aggtttgctc cggcgtcgca catgacgatc gggccggcaa aagagggcag
    20821 aacgacggcg ataccggccc gggcgacctt cggcagcagc ttcatgcgaa gctggctggc
    20881 agcggcgaag gcaccggtgt tgcccgcgct gatgattgca tccagctccc gctttgcggc
    20941 catctgtgcc atacgacaga ttgaggaatc gggcttgtgg cgaaccgcct cgaccggcga
    21001 ctcatccatg ccgatgacct ggggggtgtg catgaccgag atatttggcc acgcaccgcc
    21061 ggcgagttcc aactccttgc agatgacgtt ctcgtcgccg atgaggacga tctggtgccc
    21121 gttcagcttc gcggacgcct cgaccgctcc tcggacgatt tcccgaggcg cgtaatcccc
    21181 ccccatggca tcgatgccga tccgcatgag gtctaggact cctcttcctc gatggccagc
    21241 gccacgtctc cgctcacgta gccgcaattc tcacacgacg cgtgaggcag cttggccacg
    21301 ttgcatttcg ggcatggcac caagttgggc cgtctcaacg cgtggtgggc acgtcgcttg
    21361 cgcttgcggg ccttggatac ttttcttgca ggtagcatgt ctttttccgg taagctctta
    21421 cagtttcagt gctttcccgc gatcacccgg cgggcatcaa tcgccgtcgt gaggtcactt
    21481 ggtgattacc aaagagaggc ttgcaccccc ggccaaccat ctgtcaggcc gctgttgcaa
    21541 gcctcccgcg aaccatagac agtactaggc ttggcgaagg ctgtcaaggc ggccgcccgc
    21601 ctgtcggggc cttgtcgccg gggcagcctg ggcggctctc tgaaaacgtc gggcgcaaac
    21661 cgggaaccaa gccgcagaac cgccaacacc ggaggaggtc ggcgtgttgg ctgtcacgga
    21721 cgcttggcga aggtcgggtg ggtccagcct gtgcgggcgg acaagagggg atgggcttca
    21781 ggggtcgtcc gataacagcg aggttgcaag gcagaactag gcggattctc gcgccgtttg
    21841 aaaagagtag taggcctcgc acttgttcgg gtcaccgcaa caaacgtctc ggataaactc
    21901 gtagtagaca ttgggcggaa gcccatactc gggtcggagg gggcatttca ggcaaatccg
    21961 ggggcgggat gtgctaccga tctgggacag gatgaagact ttattggtgc cggcaaagca
    22021 cttcatggct ttgttgaggc ggttttccag ttcggaaagg tttgcccgca gttcctccaa
    22081 agtcggaggg ggtggggcgg cttggcgggt tttgtggaat cgaactgcgg tattgattgc
    22141 ctcctggacc tgctgtttgg tgatgggatg ttccacaaat gcgcggacgc ccagcttgtg
    22201 cagcttcggc tggacggggt cagcctttgg gcctagaacg gcgacgacgg gtaattgaac
    22261 gccgttatct cgcatccaag ttgcgagccg gagggcatca tcgctcttgg cttcctgccc
    22321 gacaaccaca agatccggcc ggctcttggt caagagcgag gccccggccg tcacctccgt
    22381 ggtttttttg acctcgaaat ggggccggaa cattgcttcc agcaggccca gtgttgcctt
    22441 atctcggttt atcaacaaaa ctttgggcac aaacgcctcc gtgggaggta ctgtttgttc
    22501 ggtcggttat ccaacctcat gcggctgctc ggacgagcac cgggtagggc gctcctatcg
    22561 gctcgtcgcg gagtcgttac ttattgcctg attatactcc agaggcaagt gtcttgccat
    22621 ttcaataatc gctgataaaa ggtccgacca agcagtgtgg ctccgaagcg cagccaccgt
    22681 aattcacaac aggccgagac gcaacctagc tggcggcagc gtcaggggct tttggattcg
    22741 cccggaaaag ggcggtagca accctgaaaa cgccgtttct acctggccag ttcggcgatg
    22801 atcctttcga tgagctgagg catggcggag gcgacttcct cgctcaatgt atcatccggg
    22861 ctgacactct tgggttgaac gccccagatt atcacttcat caggcattga accaatgatc
    22921 tgtgccgcag cgagcgcttc ggtcaaagcg atttcatgaa gggagacgag cgggcgcccc
    22981 gcactggcaa gatcttcaac gtgcaggcgg agaattgctc ccggctccag gcccgcatcc
    23041 actgcgtcga tgacgaagac tttgcgccgg ttgctgatga tgtccagcag atccgccccg
    23101 gccgtgccgc catcaaccag ttcgacccct ggcggcaagg ccatcccccg catggcctcg
    23161 atcagtcgca cgccaacccc ctcgtcgcgc agaaggatgt tgccgatgcc caggaccagg
    23221 ataggggctc gcttctcggg attgctcatg ccttaagagt ggcagaaatg atcgccgagt
    23281 caatggtgag ccctttgccc gcacgggccc cctgcgccaa gcggtcatcc gcatcgatcg
    23341 gggcaccgag aagaccatcg gctacaccta cgacgccgac ggcatccgct tgcagggcca
    23401 acgccctcgt cggctcatgg gttgcagaca gaatcgccga cgagggcgtc ggcgctactt
    23461 gggcatccgc accggcaaga cggtgctgaa gcggacgccc accccccagg tcacccggac
    23521 ccactacctg gtggacaaga acgccccgct ggctcaggtg gtggccgaga ccgacgagtc
    23581 cggaacggtc aaggtcaagt acgtccacgg cgacgacctg atcagccaga cccgcgaccc
    23641 gaacggcccg tcgccgatca cgagctacta ccactacgac ggccagatgt ccacgcggca
    23701 cctgaccaac gacccggccg acccgcaaac cacttcggcg caggtgaccg attcatacac
    23761 ctacgaggcc ttcggcgcgc tgtgggccca cacgggcgaa acgcccaacg acttcctcta
    23821 caccggccag caactcgacc ccaacaccgg gttctactac ctgcgggcac ggtactacaa
    23881 cccggcggcg gggaggttcg tgacgaggga cacgtttgac ggcctgaagt atgatccgat
    23941 cacgcttcac aaatatgtct atgctgcgag tcaacccctc acatatcagg atccgaatgg
    24001 agcgttccct tccctcctcg gtactgtcgg cgtcatcagc attctcctca tcgtgatgag
    24061 tttcccgaat gttgtgaatg ctccgagcat ttgggacact acgcagccag acgccagtgg
    24121 cgagatggtc atcggcctcg gcgtatccct gatccttggt cttgtagttc tcgggatcgg
    24181 tgggctaata tctagggcgg accgcctttt tgcgggccgc aacccaagcg ttctgcgatc
    24241 cggaaacatg cgcgcaagcc gcacagatgt ccggccgtaa ggattgaaag gcgcatgccc
    24301 acgcaaagcc g
//
LOCUS       DAOTPW010000061        24241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2158_length_24241_cov_5.525014, whole genome shotgun sequence.
ACCESSION   DAOTPW010000061 DAOTPW010000000
VERSION     DAOTPW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 24241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24241
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_24241_cov_5.525014"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2320)
                     /locus_tag="P5316_07475"
     CDS             complement(<1..2320)
                     /locus_tag="P5316_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84836.1"
                     /translation="MEMTTGVGETSSAGLASTPSTGQTQDCEPAGGAQRDAIRAITEE
                     RVQARMLLERTVKQCRELYEQVQQQVRIADAKLCSAESIANYLRDLHQDLAASYNPER
                     LSAFDAKSVQLSEVIALADRRQEELGDVSVAVKTLRDEVVDWTHRATRVRGSLLRTSQ
                     SAEAKETEVSRAAERAEQARLAVEAATQTAAGLVDKVYGLSQRIDEQTKTFDVRISNM
                     RQVVADLEALYERALALTSGQMESATALARRIDEQTQTFSDRLADADAATARLLHAQE
                     QATAATNEQLQAGSELAQRIQNRTEAFDLRVESAKGITSALDDACNRAVAAADRQLEQ
                     AETLGRRIDEQIQTFDKRVADSAIAAGRLDEMLLASDSMIERIEQQEQRLNERIASAE
                     TVAARMDEHCSRICRAIDEQLQAANGLSHRIQSQEDALGAYIGQMQSNIAVLNEAYER
                     ADTAGKRHLESADALAARARECAEALDARIDAARGASERLEETAARSNEVLVRHTEAM
                     QAMSEQSKEQMAALQSTIKDAVQVSEHLQHVYGRLSSDATAQLDAMNALSTHVQEQTR
                     AFDARVSEGHAVVARIEEASSRALETSGRQIETMNAALERLMDQTARLESCISTAKDA
                     AEHLTNTYSKASSDSKREIEAASALMERMQEQMRILSDRTENARSTGEQLEAVAGRVA
                     EAAAQQTQAVNGMMNRVGGLLEELAGRISDARTAGEQLERVQAQLAETAAGQSETANA
                     LAVRIAEHTRMFDSRTTEARTLADRLDEVYARV"
     gene            2976..3671
                     /locus_tag="P5316_07480"
     CDS             2976..3671
                     /locus_tag="P5316_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR84837.1"
                     /translation="MAARKKSVLIVEDEADLAELIRHNLEHEGYTCRMVSNGGSALAE
                     AVAHPPDLILLDRMLPGMSGDEFITHLKRDNRVASIPVIMLTAKAEEADQLVGLSLGA
                     DDYITKPFSMKLLLARVAAMFRRVEIASTDQEVVTRGPVSLDSSRHEVTVAGSQVALT
                     ATEFRLLRALMAANGRVLTRARLIETTMGVGVAVTDRTIDVHVASLRKKLGKAARWLQ
                     TIRGVGYAFRESP"
     gene            3697..5589
                     /locus_tag="P5316_07485"
     CDS             3697..5589
                     /locus_tag="P5316_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR84838.1"
                     /translation="MRRPRSLFSKLFLGNTVLIIAVLGICAYLIVQHVQRAYAEQVGR
                     ELRLHAHLVEAAVHAQLDPQRAEELDRLVKQFGAAADNQVRITLILPDGQVLADSQAE
                     PKTMESHATRPEVVRALSAGWGEETHYSRTLEQFQRYAALRVGSAEAPSGVIRVSMPV
                     QMITARANAFRRIIWTIVLLGAIAAILFALGLARLWSWPIRRITAIARSLSKGDLSAR
                     ARVAGPAELAMLAASLNGMRDHIADQLATIDRQRRTLESLLAQLHEGVIVAGSNGRIL
                     LVNPAAVRMLGLSGDPVEKAGSLLGQAVEQYVLQHDLQDMLLSENRRRADKRTDKPPA
                     EPAVPREVRVQIEEENGELVLMARASDIVLPVPASSGGPLERPDVGRMLVLSDITQIS
                     RMVRIKTDFAANASHELRTPLSAIRAAVETLLSLDWQNDQDAAKRFLDMIDRHSARMQ
                     QMVSDLLDLSRIESSPGQFKPQTLILSAVIEELRARFAEKLAARNLQWSLKLDPELRT
                     IDVSPHLLRVVLDNLVDNAIKFTNPGGRVQIASQKVLNGAGKIEAVIISVEDTGCGIA
                     EEEQERVFERFYQVEKARSGPDRGTGLGLSIVRHAVAAMHGRVTLTSKLGQGTCVTVS
                     IPLPRQ"
     gene            6128..7213
                     /locus_tag="P5316_07490"
     CDS             6128..7213
                     /locus_tag="P5316_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR84839.1"
                     /translation="MKQEWNTRIGSLARPAFTLIELMVVVAIIALLMAILLPSLQQAR
                     AEVKAVTCASNLHHIGQAVAIYLNRDKVYPVAYAYVDDRGRVDLSPPVQEFLQSKGQK
                     RYVHWSYFLYSNGQVSDSAFQCPAFGKGGIPRTNPGPEQADWESAQVDEMGQTGANPR
                     VDFQASRMAYTANAAIMPRNKMTPSMSGGSRVNKFANDSAIKHPGQVIMATEFNNHWP
                     AIAVNSGSGLLSKSHRPINPFYSYSSGWDEYNASDPRGAGRAAFTLGNGGPDNNYGLY
                     PLQQVLTTGGLIEESEINAVGRHHPGGDKEFGGTANFLYVDSHVDRKTIMDTMKKFEW
                     GSRYYTLSGNALVERPGPGGAVTASLN"
     gene            7271..10048
                     /locus_tag="P5316_07495"
     CDS             7271..10048
                     /locus_tag="P5316_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRR84840.1"
                     /translation="MYRKRFLVSALAVTITIIAVRAATAQSYLYNGEISIAGATLFRD
                     FFRFPASTNDYIDADGDGIAGKFNSWPPDQLAVTFVPGSPLTTHWIVQYRGVGSVNGL
                     SEFLASQGCGTVATRIPDPGYFNRFLYAQAGTYNWTGPYGDVSGTPRTPDQIDLAVLD
                     VPTDWAVLTAGGTPKWNLKPGMPGYGRCPIKSSTDWGYELADLTVSCPGGSQVVFNKN
                     VGSPDVLTIFDTPIAWVPIAIIANRGTGLHQLKASEMQYLYVTGRMPNGENLVAATRD
                     AGSGTRNGVMNTLGIDPSWGRGDNLGSETTSSSRFILGPNHQPTNSASSSRLQDVAKD
                     RRLAVGYTGLAGSTAAVAQARGGRFEILDIIFDDRGGISPVRPGIDTVLDNADPNTGY
                     QVGGPETFASLGDPFETNPAAPSYMSDQHAAKYLRNIVESVANFVSAPADPANDGMPG
                     EYLATKFFLSAGLDALPDYANFDPATFVLQPTPPFNQTLQDYMRANIDLTGGTSHVDT
                     PPFGSINKAGLTPTRTVNPPFADGITRYSDGSSNGNYCYWVGGWEQIATNVKLPESCD
                     LSGDFNQDNVRNVNDAEQMVAAYYSPRAWTVSMGFRGGQAGGPSTPSGIAADTIIPEV
                     IGDYDGDGDFGKEDLRYFADGLAIDPLTGQLNRKKGAIAVDNAILHYAPANDENGQPV
                     ADRNHWFPWKATAKRVLIPSDTPCGEPTHVLPPDVNDPADPFLATGAVYAPGDFRGDV
                     AGRSPVAGAQPLGWDGRVDAHDIDYVCQNIGNWENLDQAALIDLSADMNGDLVIDMND
                     QLEIVAGILKTRIGDVNLDGNVDAADLAIIIANQGRTGGWAMGDMNCDGVIDQNDLPP
                     RADFDGDRDVDADDWAAFELCASGPGVLPLAECQPKDFDLDGDVDQADFARFQRCFSG
                     ENVSAGPDCMD"
     gene            10287..11141
                     /locus_tag="P5316_07500"
     CDS             10287..11141
                     /locus_tag="P5316_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRR84841.1"
                     /translation="MKTTRSLLAGAFLVATSILSTGPAQADDKAKDTNKIVIDGSTTV
                     GPIAKAFAEYYMRQNPGVNITVSESGSGNGAKSLVNGTCDIASMSRFMKDEEVRAAIE
                     KGVVPVCHTVAVDGIAVIVHPSNPIKGLSLAQVRDIYTGKVTNWNQVGGPNAKIVVIS
                     RDSSSGTYESFETLVMNKEKMAGSVEYVGSSGAIRQRVQSTQGAIGYVGIGFTDRTVK
                     ALEIDKVMPTKATVASGRYPIARPLFLFTNGYPAMGSHLYNFTNLYLSRKGQEMIEAI
                     GFVPLTEY"
     gene            11191..12420
                     /locus_tag="P5316_07505"
     CDS             11191..12420
                     /locus_tag="P5316_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstC family ABC transporter permease"
                     /protein_id="HRR84842.1"
                     /translation="MGKPPVMSGEKDMRVMESVGEISGLRTMRRPSLLISRKAGLVRW
                     IAGRLGQGLLLIVTCSSILIVLFIFYFIARDSIPFFRAQGWSGAREFFTSTAWYPTGT
                     PAHFGALAIFYGSAMVTLGATLVAVPLGVAAAVCLSDVLPFWLRQWTKPVIELLAAIP
                     SVAFGFFALVVFATQLQDNGGVILSTAWWLIATPILSLAVFVATEVMTAGIQTPSTRR
                     IARGIVGALLVCLAGGLLWWVSTSLRALHVDSGCNALNVSIILGIMALPTVVSVSEDA
                     LQAVGRELREGSYALGATRAETMLRVVIPAAGSGICAAVILGIMRAVGETMVVWMASG
                     NASRIPSPRYDFLQPIRTLTATIAGEMGETDQMTGAAHYSSLFLMGFCLLVISFLFNL
                     ISEAVVRRSVMGKGGKR"
     gene            12423..13772
                     /gene="pstA"
                     /locus_tag="P5316_07510"
     CDS             12423..13772
                     /gene="pstA"
                     /locus_tag="P5316_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRR84843.1"
                     /translation="MALTSRKLTDRLFTFVGVSSILLLAAFLLVLLTPITLGGIGAFV
                     FRGTVEHRRMQLELFETGKKADLEADMAAAFEARRPVYEMIAAFEKEIEAQGFLASRK
                     YAEPLQAVKDELRLLLGPAPGEPKPVMYRDQYGQTRWDRARVVLHHLLYDKQYDYSNP
                     DEMGKPIDVPRVNEFKGTALEPLFAYVEQNLDKMLLPRVTLYWGFLTQTSYDSHYFGG
                     VWAELLGTFYLTVGAILFAVPMGVVSAIYLVEYAPATSKLVQLIRTCISTLAGVPSIV
                     FGLFGLAFFINTVGVSQSKSVLAGSLTLSILILPLVIRASEEAIRAVPHTYKEASLSL
                     GAGKWRTIVRVILPSALPGILTGIVISMGRAAGETAPIIYTAAVSVGLPLSLRDTLTQ
                     PTPALPWNIYNLCTEHQRVDEIRHVQYGMVFTLVMLVLLLNSVAIYARAKISKRLRG"
     gene            13871..14617
                     /gene="pstB"
                     /locus_tag="P5316_07515"
                     /pseudo
     CDS             13871..14617
                     /gene="pstB"
                     /locus_tag="P5316_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
     gene            14622..15290
                     /gene="phoU"
                     /locus_tag="P5316_07520"
     CDS             14622..15290
                     /gene="phoU"
                     /locus_tag="P5316_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRR84844.1"
                     /translation="MATHFINLLDELMRRSLRMGAAVEDMLLEACEAVTHADHNLARR
                     IIDRDAEIDAEEVAIEAEAVRLMTLFQPMGADMRRLCTILKVNNDLERIADCGVNIAE
                     RAAHLEPEALVEARNNLKEIYPVVRRMLHGALHAYATLDRQAAERVVAEDEVIDAFYG
                     DFIRHLVIDAARSPQAMAAHLDILSIAKNLERTADHAVNIAEDVIFLVTGQIVRHTNK
                     KVSG"
     gene            complement(15498..16082)
                     /locus_tag="P5316_07525"
     CDS             complement(15498..16082)
                     /locus_tag="P5316_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR84845.1"
                     /translation="MQKLAVLGGALAMCMSWASAVRAVTVTIYTDKTQWESALGGQFL
                     SEDFADSQLNDGVSFVSTESGHINPAEECYQDVLASQSQNEPMTIWSFSPEIAAYGGD
                     WALGGPGGSGNSLLVYIADFSLYVGAISNNYGGEFWGFISDTPFTSVRLVGGPGSHQQ
                     NYRLDNMVYSQVPEPAAMSLLTLGGLMLIRRRTA"
     gene            complement(16370..17407)
                     /locus_tag="P5316_07530"
     CDS             complement(16370..17407)
                     /locus_tag="P5316_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRR84846.1"
                     /translation="MRRLQRRRVVAGSGLSVVTAWLAIAGCSVPGFEKEDERPLQAPE
                     VPVSSPSITSFPATAPVEGPWAAPAPPVVQSAPAIATQPAQPPPPDPLDTACQEGMLA
                     WEQGDLVRARYHLNRVLREGYPSERQRRVREILNLIADRTIFSPEILPGDPLVVSYRI
                     VKGDTLSRIARKHGLTEELISSINRLSAGSVLRPGHQIKLVRGPFSATVVKSDHEMHL
                     YLQDVYVRTCGVALGTDNKTPTGKWVVTTRLRHPSWVDPRNGRRYGSHDPDNPLGGYW
                     IALKGIEGNAVGRRGYGIHGTNDEASIGTDASLGCVRLGKQDIEAVYAMLARDCIVTI
                     IESGERRGQIE"
     gene            complement(17450..17761)
                     /locus_tag="P5316_07535"
     CDS             complement(17450..17761)
                     /locus_tag="P5316_07535"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRR84847.1"
                     /translation="MAYVHCVFFTLKPGTPQSQIDAQVVGGRELLARIPTVRRLETGR
                     RDETMCREVSVTDFDLGLVILFDDKAGHDVYADHPLHLEYIKRHKARWARIRVFDYLS
                     A"
     gene            18022..18420
                     /locus_tag="P5316_07540"
     CDS             18022..18420
                     /locus_tag="P5316_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRR84848.1"
                     /translation="MKHVIALALALALNAAANLMMKIGSGRFNASGLNTDPGLRSLAA
                     ALTANWVLVLGLLCFAINVMFYTYALRAAFLPVSMAYPIMVGGGFAIIALVAWWFLGE
                     RMSVVQWTGVVMILLGVFLVAREVRVDSGV"
     gene            18437..19750
                     /locus_tag="P5316_07545"
     CDS             18437..19750
                     /locus_tag="P5316_07545"
                     /inference="COORDINATES: protein motif:HMM:NF021584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HRR84849.1"
                     /translation="MRWFKKTIPWRGEVVFRGSLQPRAADFEYLEKHRIRIRPGNGDP
                     EFLWSLTLEHPDWGTATLQCPRETHRIDPAILDHDWLLSETERQEARLGESTVLLGMP
                     SAKGHMLRDRKLALRFMRAIMGDDGVLGLDCLGWRTWTRTGLDEELAHDADLDVEAIH
                     SLHWVKTESGEEAGWLHSHGLAEIGGFDFDILRPAKELATSACDALRAIAFAILEERV
                     STSTTRFSLAMPGGDVQFVDVDRFNALASAADRALRTADDPAHSRKRAVLCDPAGGFF
                     SRFSRKVRPSSYLAGPIDDRAVICFSGSATDLMAERARKTYPVLRALLDELAEFEFPV
                     GAKIGYPTDHRTGPDDREHLWFSIHRCFDDKIDATLENSPLDIAGMNAGDRAEHPVEL
                     LTDWIIMTPFGQINPRSMVVVRKIREHREELRAIMAEYRRQSSKA"
     gene            complement(19736..20206)
                     /locus_tag="P5316_07550"
     CDS             complement(19736..20206)
                     /locus_tag="P5316_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR84850.1"
                     /translation="MDDNLIIRAAGAVDVPVITEFIIAQALESEGLRLDPETARRGVA
                     HAVAHPQQGRYFVAECDGRVIGQLMITREWSDWRARVFWWIQSVYVHPDYRGRGIFRR
                     LYRHVESLAQQDPGICGLRLYVHRDNHRAMAAYEKLGMRATNYRVYEVKWSGLG"
     gene            complement(20360..22582)
                     /locus_tag="P5316_07555"
     CDS             complement(20360..22582)
                     /locus_tag="P5316_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84851.1"
                     /translation="MARRPDPQSEGRPADHDSSAHGLLGDVRRKLDQIERELTERERK
                     LAEAESRLRDEQARLRKEQAAWSSQHGREMEAQAKLEQAAKLLAKQEALLKDREEQLR
                     VFAAKNQELREQASRLGRDRDETRKRLAEAVEEAEIQSRRAAGLEKQIAVLKNTMETE
                     SAVPAQPTTFFAGTSQPDREAATIDKPGRSESSAALVAFGIIAVLVSAGLGVWSHATY
                     KPLYTVNGHIKTRPGDAVTLSACADLAKADGLPIQEMDVPRGLLTFRLQTREPDQAAA
                     RMNQTARHMVALYRSPASAPASRPSSTPQRERIRQQIAEIEHRLEIATRPAFAEQEEG
                     ARLVAQWTAAEAQRREVEAALASLSNAETGAEIDPASVRIDARQIQAAVTEDRKLSSE
                     IQALQQREAELSQALKRITDGSDGTFRTLERAIADGTARIDRAMKEEYGARIRESLSD
                     LKQALAAWNEAATAMSSDWAAQRDALAGEADLLTCQTELDRAARQFIDAGSAVLIQFR
                     QRLDAISQDTDEPTKGLVLGRALTKDLQPVVDAQQEIVRLARSATLTGNLDLATIVQQ
                     VAGLRRQVNDRRTEIEASLRAKMLDDLKQDREQTIRQRREQLTQRAEDLDREIRGHVE
                     NVRALVRRMDQRQAELAEWRRLQDERAAALRSLADVQEQEPPAGPAVEPVFIEFSGTG
                     IDPGEPGDRRVVRSLLAGFGTLFVALVGWLSVHSYRSWHRGRQAIEACTRELRAATRE
                     "
     gene            23186..23515
                     /locus_tag="P5316_07560"
     CDS             23186..23515
                     /locus_tag="P5316_07560"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRR84852.1"
                     /translation="MIIALNLFDIIPGKEAVYAEYLRRVRPILERHHARVLFYGRTRA
                     RFMGACNQEYCGVIAYEDPTDLTRFSHDPEFAEIKPLRDASTHNYVLTVAEETGIEDA
                     AEMLSRS"
     gene            23655..24026
                     /locus_tag="P5316_07565"
     CDS             23655..24026
                     /locus_tag="P5316_07565"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRR84853.1"
                     /translation="MQEYLAITKALGDETRVRALLALRGGELCLCHIIQLLRLAPATL
                     SKHMDLLVQAGLVERRKDGRWCYFRLIGANAQPAARRALRWTLDALADDPVIRRDAEA
                     LNKIRCADPKELSACYRSGKY"
     gene            24002..>24241
                     /locus_tag="P5316_07570"
     CDS             24002..>24241
                     /locus_tag="P5316_07570"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRR84854.1"
                     /translation="MLQKRKVLILCTGNSARSQMAEGFWRHLGGDRWEVFSAGVDPKG
                     VNPVAVAVMAEAGVDISAHPLAPVLSSAKTPLANLS"
BASE COUNT         4550 a         7652 c         7228 g         4811 t
ORIGIN      
        1 atacccgggc gtagacctca tccagacggt cggccaacgt cctggcttcc gtcgtcctgg
       61 aatcaaacat ccgggtatgc tcggcgatcc tgacggccag cgcgttggcc gtttccgact
      121 ggccggcggc agtctcggct aactgtgcct gaactcgctc aagttgttcg ccggcggttc
      181 gagcatcact gatccgcccg gcgagctctt ccagcaatcc tccgacgcgg ttcatcatcc
      241 cgttgaccgc ttgcgtctgc tgagccgccg cctccgcgac acgccccgcc accgcttcaa
      301 gctgctcgcc ggtgctgcgg gcattctcgg tccgatcgct caggattcgc atctgttcct
      361 gcattcgctc catcaaggcg cttgccgctt cgatttctcg cttgctgtcg gaagaagcct
      421 ttgaataagt gttcgtgaga tgctcggcgg cgtccttcgc ggtcgaaatg cacgactcaa
      481 gccgggccgt ctggtccatc agtcgctcta aagcggcgtt catggtttct atctgtcggc
      541 cggatgtttc cagagcccgc gacgaggcct cttcgatccg ggccacaacg gcgtggcctt
      601 cgctgacgcg ggcatcaaaa gcccgcgttt gctcctgcac gtgcgtcgat agggcgttca
      661 tggcatcgag ttgcgccgtc gcatcggagg acagtctccc gtaaacgtgc tggagatgct
      721 cgctcacttg tacggcgtcc ttgatggtcg actgcaaggc agccatctgt tccttgctct
      781 gctcggacat cgcctgcatc gcttccgtgt gacggaccag gacctcgttt gatcgggcgg
      841 cggtctcctc cagtctttct gatgcgcctc gagccgcgtc gatgcgtgcg tcgagtgcct
      901 ccgcgcactc gcgggcacgt gcggccagcg cgtcggcgga ctcgaggtga cgcttgccgg
      961 cggtgtcggc tcgctcatag gcctcgttca gcaccgctat gttgctctgc atctggccga
     1021 tataggctcc cagcgcatcc tcctgtgact gaatacggtg ggacaggccg ttggccgctt
     1081 gaagttgctc gtcaatcgcc cggcagatcc ggctgcagtg ctcgtccatg cgcgctgcta
     1141 ccgtctctgc tgacgcgatt cgctcgttca gccgctgctc ctgctgttcg attcgctcga
     1201 tcatcgaatc cgaggccagg agcatctcgt cgaggcggcc cgcagcgatg gccgagtcgg
     1261 caacccgctt gtcgaaggtc tgaatctgct cgtcgatgcg ccgacccagc gtttcggcct
     1321 gttccagttg ccgatccgcg gccgccacgg ctcgattgca ggcatcatcc aaggctgagg
     1381 tgattccctt ggcgctttcc acccgaaggt cgaaggcttc cgtccggttc tgtatccgtt
     1441 gagccagctc ggagccggcc tgcaactgct cattggttgc ggccgtagcc tgttcctgcg
     1501 catgcaacaa ccgcgcggtc gccgcatcgg catccgccag ccgatcactg aacgtctgag
     1561 tctgctcatc aatgcggcgg gcaagagccg tcgccgactc catctgtccg ctggtgagcg
     1621 ccagcgctct ttcatacagc gcttcgagat ccgccacgac ctgccgcata ttgctgattc
     1681 gcacatcgaa ggtcttggtc tgctcgtcga ttcgttgcga gaggccgtag accttgtcga
     1741 caagtccggc ggccgtctgg gtcgccgctt caacggccag gcgggcttgt tcggctcgct
     1801 cggccgcgcg gctgacctcg gtttctttgg cttcggcaga ctgcgaggtg cgaagcagcg
     1861 agccgcggac tctcgtcgcc cggtgcgtcc agtcgacgac ctcatcgcgc agcgttttca
     1921 ccgcgaccga gacgtcgccc agttcctcct gccgccggtc cgccagtgca atcacttcgg
     1981 acaactgcac actcttggca tcgaaagcgc tcagcctctc ggggttgtaa ctcgccgcca
     2041 gatcctggtg caaatcgcga agataattcg cgatgctctc ggcgctgcac agcttggcat
     2101 cagcaatccg aacctgctgc tgaacctgct cgtacagctc gcggcattgt ttgaccgttc
     2161 gctccagcag catgcgggct tgaacccgtt cctcggtgat ggcacggatg gcatcccgct
     2221 gggcgccacc ggccggctca caatcctgcg tctgtccggt cgagggggtc gaagcaagac
     2281 cggcggacga cgtctcaccg acgcctgtcg tcatctccat aggttcatct cccgatgagt
     2341 caaggctcgt ttcatgttgc atgacatcac ctttccgaca catccggcag gcgtagtaac
     2401 cgcggatcgg tcgcatcgca gaaaggattc ttggtcagac ggcagaactt cttgtacatg
     2461 cctgtacgcc tcaccatccc tgggcaagtt ctccaagaca taatcggaca ttgccttctc
     2521 ggacctaagc taccgtgccc acgaaaacgg tcctctgagg cttggtcttt ccgttccctt
     2581 gcagatccgc cggtcgccgg cggaatcctc gcatgcgtgt gaacgcttgc tctcaggttc
     2641 cttccctccg gctgttccgc agagtcccat tttcccggac gttggtcggc caggcctgtc
     2701 agactcctca ggacaccgtc ggtcttccgt tgcggtcgta gaccagcctc gggtaacact
     2761 tgctttcctg ctatcagcga tgagaatgtc ctataaacgg gaacgaatcc cttggtgacc
     2821 gagacgggca tgtttaacca attcctaaca taatccttac gcgggattag cccaacgttt
     2881 gtaaaagagc ttcatcgatt gtggcaaaag ggggcaaaac caatcggccg caagggcaac
     2941 cgcagtccgc gttcggcttc tgagggtaga tgttgatggc agcgagaaaa aagagcgttc
     3001 tgatcgttga ggatgaagcc gatttggcgg agttgatccg ccacaacctc gaacacgaag
     3061 gatatacctg ccggatggta tccaacggcg gttcggcgct ggccgaggcg gtggcgcatc
     3121 ccccggattt gatcctcttg gaccgcatgc ttccaggcat gtccggcgac gagttcatca
     3181 cgcacctcaa gagggacaac cgggtagcct cgattcccgt catcatgctg accgccaagg
     3241 cggaggaggc ggaccagttg gttggcctga gcctcggtgc cgatgactac atcaccaagc
     3301 cgttctcgat gaaattgttg ctggcccgtg tcgcggcaat gttccgccgc gtggagatcg
     3361 cttcgaccga ccaggaggtc gtgacaaggg gccctgtctc gctcgacagc agtcgccatg
     3421 aggtcacggt agcgggctcg caggtcgcgt tgactgcgac ggaattccgc ctgcttcgcg
     3481 ccctgatggc cgccaacggg cgggtactga cgcgtgctcg tctgatcgag accaccatgg
     3541 gtgtcggtgt cgccgtgacg gatcgaacaa ttgacgttca cgtggcttcg cttcgaaaga
     3601 agctgggcaa ggcagcccga tggcttcaga ccatccgcgg tgtcgggtac gctttccgcg
     3661 agagtccttg agatccggcc tcgaaaggca tggctgatga gacgcccgcg ctcgctcttc
     3721 tcgaaactct ttctcggcaa cactgtgttg atcattgctg ttctcgggat ctgcgcatac
     3781 ctgattgttc aacacgtcca gagagcctat gccgagcagg ttggccggga gctccggctt
     3841 catgctcacc tcgtcgaagc tgcggttcat gctcaactcg atccgcagcg tgccgaggag
     3901 ttggatcggt tggtcaaaca gttcggtgcc gccgccgaca accaagtccg gatcacattg
     3961 attctgcccg atggtcaggt gttggcagac tcacaggccg aacccaagac gatggagtcc
     4021 cacgccactc gccccgaagt ggtaagagcc ctgagcgcag gctggggcga agagactcat
     4081 tactccagaa cgcttgagca attccagcgg tatgctgcgc ttcgggtcgg atcagccgag
     4141 gctccgtcgg gcgtgattcg tgtctctatg cctgttcaga tgatcacggc cagggccaac
     4201 gccttccgtc ggatcatttg gaccattgtc ctgctcggag cgattgcggc gatactcttt
     4261 gcccttggtt tggcccgcct gtggagctgg ccaatccggc ggatcacggc aattgcccgc
     4321 agtctttcaa agggcgatct ttctgccagg gcccgggtcg ccggccccgc agagctggcc
     4381 atgctcgccg catccctcaa cggcatgcgc gatcacatcg ccgatcaact ggcgaccatc
     4441 gatcgccagc gccgaacgct cgaatcactg ctggcccaac tccacgaagg cgtcattgtg
     4501 gcaggcagca acgggcgcat cctgctggta aacccggcag cggtccgaat gctcggcctc
     4561 agcggcgacc cggtcgagaa agcaggatcc ttgctcggac aagccgttga acaatacgtt
     4621 ttgcagcacg acctgcagga catgctgttg tcggaaaaca ggcgccgcgc ggacaaacgg
     4681 accgacaagc cgcctgctga accggcggtt ccgcgtgagg tccgggttca gatcgaggag
     4741 gagaacggcg agcttgtgct gatggcccgc gcatccgaca ttgtcctgcc ggtgcctgcg
     4801 tccagcggcg ggccgctcga acggccggat gtcggccgga tgctggtcct gtccgacatt
     4861 acccagatca gccgaatggt ccggatcaag actgattttg cggctaacgc ctcacacgag
     4921 ctgcgaacgc ccctctccgc gatacgcgcc gccgtcgaga ccctgctcag tcttgattgg
     4981 caaaacgatc aggatgccgc caagcggttc ctggacatga tcgaccgtca cagcgcccgc
     5041 atgcaacaga tggtatccga cctgctggac ctttcccgga tcgagtcctc ccccggacaa
     5101 ttcaagcccc aaacgctcat cctgtcggcg gtgatcgagg aactcagggc ccgattcgcc
     5161 gaaaaacttg ccgccaggaa cctgcaatgg tcgctcaagc tcgaccccga gctgcgaacc
     5221 atcgacgtca gtcctcactt gctgcgggtg gtgctcgaca acctggtgga caacgcgatc
     5281 aaattcacca acccgggcgg ccgcgtgcag atcgcttctc agaaagtact gaacggagcc
     5341 ggcaagatcg aggcggtcat catctcggtc gaagatacgg gttgcggaat cgccgaggag
     5401 gaacaggagc gggtcttcga gcgattctac caggtcgaga aagcccgcag cggccccgac
     5461 cgcggaaccg gcttggggct ttccatcgtg cgccatgccg tggccgccat gcatggccgc
     5521 gtcactctga ccagcaaact cggccaagga acctgtgtga ccgtcagcat ccctcttcca
     5581 cggcaataat cacgtcgtcg gcacaccctg ccgaaatgtc acgccgctcg ccggagcttt
     5641 cagtgcgcaa gacgagcccg gtctgacccc tccggcaaga ccgggctgac gtctggcacc
     5701 gtctgtccga cgagtgcctc ttgcgcggat tctgacgtga gatggaagtg atgtcattct
     5761 caatatgaaa ccggcccgtg agacctgtgt gtggagctgg tcagaatctc gcgaattcgc
     5821 accgattcgc gctcgccgca ggcgccgctc ttgaccagtt atccggtggc gcgattgggt
     5881 ccggcgcgtt gacgggtatt cgccgcgcct cctggatcgc ctcgctcgtt ggtgatcccc
     5941 gctcgtgcct gatccggcaa gtcttgtggg atttcgtgct ttttctgatc agtggtgaag
     6001 ggattggggc gaccggcggg gaattgttca ttttttctcc gggtgctcat ctcggcttag
     6061 cgcaccatct cgacaatacc cgccgtgtta gcacggattt ctgttgtgca ttgtaaggag
     6121 tctcataatg aaacaggaat ggaacacacg gatcggtagc cttgcaagac cggcttttac
     6181 tctcatcgag ttgatggttg tggtggccat cattgcactg ctcatggcca tccttttgcc
     6241 gagcctgcag caggcgcgtg cggaggtcaa agccgtgacc tgtgccagca acctgcatca
     6301 catcggccag gcggtcgcca tttaccttaa tcgggacaag gtgtacccgg tcgcctatgc
     6361 ctacgttgat gatcgcggcc gagtggacct cagtccgccc gttcaggagt tcctgcagag
     6421 caagggccag aagcggtacg ttcactggtc ctatttcctt tatagcaatg ggcaggtgtc
     6481 ggacagcgcc tttcaatgcc ctgccttcgg caagggcgga atccctcgga ccaaccccgg
     6541 acccgagcag gcggactggg agtcggcaca ggttgacgag atgggccaga ctggagccaa
     6601 tcctcgcgtc gactttcagg cttcgcgaat ggcctatacg gccaatgcgg cgatcatgcc
     6661 aagaaacaag atgaccccgt cgatgtccgg cggcagccgc gtcaacaagt ttgcgaatga
     6721 ctcggccatc aaacaccccg gacaggtcat catggcaacc gaattcaaca accactggcc
     6781 ggcgatcgcg gtcaattccg gcagcggcct cctcagcaag agtcatcgac cgatcaatcc
     6841 cttctacagc tacagcagcg gctgggatga gtacaacgcg agtgaccctc gcggggccgg
     6901 ccgtgcggcc tttacacttg gaaatggcgg accggacaac aactatggcc tctatccgct
     6961 tcagcaggta ctcaccacgg gcggactcat cgaagagtcc gaaatcaacg ccgttgggag
     7021 acatcatccc ggcggggaca aggaattcgg aggtacggcc aactttctct acgtcgattc
     7081 ccacgtcgat cgcaagacaa tcatggacac gatgaagaag tttgaatggg gctcgcgtta
     7141 ctacacactg agcgggaacg ccctggtgga gcggcccggg cccggcggcg ccgtgacggc
     7201 gtcgctgaac taaggatatc ccaaagctga ccctcgtcgt ctatttgatg aaagaaagga
     7261 gaacggaaag atgtatcgga aacggttctt ggtatccgcc cttgcggtca cgatcacaat
     7321 catcgcagtg cgggctgcca ctgcacagag ttacctttac aacggggaga tctcgatcgc
     7381 cggtgctacg ctttttcggg actttttcag gttcccggcc agcaccaacg actatatcga
     7441 cgccgacggc gacggcatcg cgggcaagtt caatagctgg cctccggacc agttggctgt
     7501 cacctttgtg cccggcagcc ccttgacaac ccactggatc gtccaatatc gcggcgtggg
     7561 ttcggtgaac gggctgagcg agttcctggc atcccaaggc tgcggcaccg tcgccactcg
     7621 aattcccgat cctggctatt ttaaccgctt cctgtacgcc caggcgggaa cctacaactg
     7681 gaccggccca tatggcgatg tcagcgggac tcccagaacg cctgatcaga tcgatctggc
     7741 ggttcttgac gtgcccaccg actgggccgt cctcaccgcc ggcggcacac cgaaatggaa
     7801 tctgaaaccc ggcatgcctg gctacggacg ctgccccatc aaatcgagca ccgactgggg
     7861 atatgagttg gccgacctga ccgtgagctg tcccggcggc tcccaggtcg tcttcaacaa
     7921 gaacgtcggc agccccgatg tcctcaccat ctttgacacg cctatcgcct gggtgcccat
     7981 cgccatcatc gcaaaccggg gcacgggcct ccatcaactc aaggccagcg aaatgcagta
     8041 cctgtacgtg accggccgaa tgcccaacgg cgagaacctc gtcgcggcta ctcgtgatgc
     8101 cggctccggc acgcgcaacg gcgtcatgaa caccctcggc atcgacccga gctgggggcg
     8161 cggtgacaat ctcggaagcg aaacgacgtc gtcatcccgc ttcatcctgg gccccaatca
     8221 ccagcccacc aactccgctt cgtccagccg gttgcaggat gtcgccaagg accgtcggct
     8281 tgcggttggc tataccggtc tggccggcag caccgcggcg gtggcgcagg ccagaggcgg
     8341 gcgattcgag attcttgata ttatctttga tgatcgcggc ggcatcagtc ccgtgcgtcc
     8401 cggcatcgat accgtcctgg acaacgcgga cccgaacacc ggttatcaag tcggcggccc
     8461 cgaaaccttt gcctcactgg gcgatccttt cgaaacgaac ccggccgctc caagttacat
     8521 gagcgaccag catgcggcca agtatctccg caacatcgtg gaaagcgtcg ccaacttcgt
     8581 tagcgctccg gcggatcccg ccaacgacgg catgcccggc gaatacctgg cgaccaagtt
     8641 cttcctgagc gccggtcttg acgccctgcc cgattacgcc aactttgacc cggctacctt
     8701 cgtgctgcag ccgacgccgc ctttcaacca gaccctgcag gactacatgc gggccaacat
     8761 tgacctgacc ggcgggacca gccatgtgga tacgcccccg ttcggcagca tcaacaaagc
     8821 cggcctcacg ccgacgcgta cggtcaaccc gccgttcgcg gatgggatca ctcgttacag
     8881 cgacggcagc agcaacggta actactgcta ttgggtcggc ggttgggagc agatcgcaac
     8941 caacgtcaaa ttgcccgaaa gctgcgactt gtccggcgat ttcaaccagg ataacgtccg
     9001 caacgtcaat gatgccgaac agatggtcgc ggcctactac agcccgcgag cttggaccgt
     9061 gtccatggga tttcgcggtg gtcaagccgg tggtccaagc actccttcgg gcatcgcggc
     9121 cgacaccatc atacccgagg tgatcggtga ctatgacggc gacggcgatt tcggcaagga
     9181 agaccttcgg tacttcgccg acggcctggc catcgacccg ctgaccggac agctaaaccg
     9241 caagaaggga gcgatcgcgg tcgacaacgc cattctgcac tacgcgcccg caaacgacga
     9301 aaacgggcag cccgtggccg accggaacca ctggttccct tggaaggcaa ccgccaagcg
     9361 ggtgctcatt ccgtcggaca caccgtgtgg cgagcccact catgtcctgc cgccggatgt
     9421 caacgatccg gctgacccgt tcctggcgac cggcgcggtc tatgcccccg gtgacttccg
     9481 cggtgacgtg gcaggccgtt cccccgtggc tggtgcccag ccgctcggtt gggacggtcg
     9541 ggttgatgcc catgacatcg attacgtgtg ccaaaacatc ggtaattggg aaaaccttga
     9601 ccaggcggcg ctcatcgacc tcagcgccga catgaatggt gatctggtca ttgatatgaa
     9661 tgaccaactt gaaatcgtcg cgggcatcct gaaaacccga attggcgatg tcaatctgga
     9721 cggcaacgtt gacgccgccg atttggccat catcatcgca aaccagggcc ggacgggagg
     9781 ctgggcgatg ggtgatatga actgcgacgg cgtgatcgat cagaacgacc ttcccccgcg
     9841 agcggatttt gacggcgatc gagatgtcga tgccgacgat tgggccgcgt tcgagctctg
     9901 cgcgtcaggc cctggggtct tgccgcttgc cgagtgtcag cccaaagact tcgacctgga
     9961 tggcgatgtc gaccaggcgg acttcgctcg ctttcaacgg tgcttcagcg gagaaaacgt
    10021 ctcggccggt ccggactgca tggattgatt cgtccgccca gaccgcttag cgccgcgtgc
    10081 tgttgccgca gcggaggcct cggcgagggg aaagaagtcg ccgaggcctc ttttcatgcc
    10141 gttcccggcg cgggtcggct taacaatctt aatgagttct taacgcgcgg cgaaccccgt
    10201 cttaacgtcc tgtgtccaga atgagaacgg tacagacaac ttgcagttga gaattgtgag
    10261 aagccagcat gataggagaa aagtcaatga agacgacgag atcgcttctg gcaggggcat
    10321 tcctggtcgc aacatccatc ctttcaaccg gcccggccca agccgacgac aaggccaagg
    10381 acacgaacaa gatcgtcatc gacggctcga ccaccgtcgg cccgatcgcc aaggcctttg
    10441 ccgagtacta catgaggcag aacccgggcg tgaatatcac cgtcagcgag tcgggcagcg
    10501 gtaacggggc caagtcgctg gtcaacggca cctgcgatat agcctccatg tcgcggttca
    10561 tgaaggatga agaggtcagg gccgcgatcg agaaaggcgt cgtgcccgtg tgccacacgg
    10621 tggccgtcga tgggattgcc gtcatcgtgc acccgagcaa ccccattaaa ggactctcgc
    10681 tcgcacaggt tcgcgacatt tataccggta aggtcaccaa ctggaatcag gtcggcggcc
    10741 ccaacgccaa gatcgtggtc atcagccgcg acagcagcag cggcacctat gagagctttg
    10801 agaccctggt gatgaacaag gagaaaatgg ccggctcggt tgaatacgtc ggcagcagcg
    10861 gcgcgatccg ccagcgggtg cagtccacgc aaggggccat cggctatgtc ggcatcggtt
    10921 ttaccgacag gacggtcaag gcgctcgaga tcgacaaggt catgccgacc aaggccacgg
    10981 tcgcttcggg ccgctacccg atcgcccggc cgttgtttct gttcaccaac ggctacccgg
    11041 ccatgggcag ccacctgtac aacttcacca acctgtacct gagcaggaag gggcaggaaa
    11101 tgattgaagc catcggcttt gtgcctttga ccgaatactg atccatctgc acgggatcga
    11161 cggcggctgg cgagcgactg gatcgcaccg atgggcaagc cgccggtgat gtccggcgag
    11221 aaggacatga gggtcatgga atcagtcggc gaaatcagcg gactgcggac catgcgcagg
    11281 cccagcctgc ttatcagcag aaaggccggt cttgtccgtt ggattgccgg tcggctgggc
    11341 cagggcctgc tcttgatcgt cacttgcagc tcgatcctca tcgtcctctt cattttctac
    11401 ttcatcgccc gggactcgat cccctttttt cgggctcagg gctggtcggg ggccagggag
    11461 ttcttcacga gcaccgcgtg gtatcccacg gggactccgg cccacttcgg cgcgctggcc
    11521 atcttctacg gcagcgccat ggtgaccctc ggggcgacgt tggtggccgt accgctcgga
    11581 gtggcagcgg ccgtgtgcct gagtgacgtt ctacccttct ggctgcggca gtggaccaag
    11641 ccggtcatcg aactgctggc cgcgattccg tccgtggcgt tcggcttctt cgctctggtc
    11701 gtcttcgcca cccagctgca agacaacggg ggagtcatcc tttcgaccgc ctggtggctg
    11761 atcgccacac cgatactgtc gttggcggtg tttgtcgcga cggaggtcat gaccgcgggc
    11821 atccagaccc cctccactcg ccgcatcgcc aggggcatcg ttggggcatt actcgtgtgc
    11881 ctggcgggcg gtcttctctg gtgggtctcc acttcgctgc gggcactgca cgtcgacagc
    11941 ggatgcaatg ccctcaacgt ctcgatcatt ctcggtatca tggccctgcc gaccgtcgtg
    12001 agcgtgtcgg aagatgccct gcaggccgtc ggccgcgaac ttcgcgaggg atcttatgcc
    12061 cttggagcca ctcgggccga gacgatgctg cgcgtcgtca tacccgcggc cggcagcggg
    12121 atctgcgcgg ccgtcatcct cggcatcatg cgggccgtcg gcgaaaccat ggtcgtctgg
    12181 atggcctccg gtaacgcctc caggatccct tcaccgcggt atgacttcct ccaacccatc
    12241 cgtaccctga cggccaccat tgccggcgaa atgggtgaga ccgaccagat gaccggggcg
    12301 gcccactaca gctctttgtt cctcatgggt ttctgcctgc tggtcatttc attcctgttc
    12361 aatctgatca gcgaggccgt cgttcgacgc tcggtcatgg gcaagggagg caaacgctga
    12421 tcgtggcact gacgtcgcgc aaactgaccg atcgcctgtt taccttcgtg ggcgtcagct
    12481 cgatcctgct gctggcggcc ttcctgctgg tgttgctaac gcccatcacc ctcggcggga
    12541 tcggggcgtt tgtcttccgc ggcaccgtcg aacaccggcg tatgcaactc gaactgttcg
    12601 agaccggtaa aaaggccgac ctcgaagccg atatggccgc cgccttcgag gcccgcaggc
    12661 ccgtgtacga aatgatcgcc gcattcgaga aggagatcga ggcgcagggc ttcctcgcct
    12721 cgcgcaagta tgccgaaccg ctccaggcgg tcaaagacga gctgcgcctg ctgctcgggc
    12781 cggcacccgg cgagcccaaa ccggtgatgt accgcgatca atacggtcag actcgctggg
    12841 atcgggcccg cgtcgtgctt catcacctgc tctacgataa gcagtacgac tactccaatc
    12901 ccgacgaaat gggcaaaccc atcgacgtgc cgcgggtcaa cgaattcaaa ggtaccgcgc
    12961 tggaaccgtt atttgcctat gtcgagcaga acctcgacaa gatgctcctg ccgcgcgtga
    13021 ccctctattg gggctttctg acgcagacat cctatgactc ccactatttt ggcggcgtct
    13081 gggcggaact cctggggacc ttctatctga cagtgggagc aatcctgttc gccgtcccga
    13141 tgggcgtggt gtcggcgatc tacctcgtcg agtacgcccc tgcgacgagc aaactggttc
    13201 agttgatccg cacctgtatc agcacgctgg cgggcgtgcc gagcatcgtc ttcggcctgt
    13261 tcgggttggc tttcttcatt aatacggtcg gcgtctccca atccaagagt gtcctggccg
    13321 gttcgctcac cctgtccatt ctgattctgc cgctggtgat aagggcttcc gaagaggcga
    13381 tccgcgcggt gccgcatacc tacaaagagg cctcgctgag cctgggcgca ggcaagtggc
    13441 gcaccatcgt gagggtcatt ctgccctccg ccttgcccgg catcttgacc ggcatcgtga
    13501 tcagcatggg ccgggccgcg ggcgagacgg ctcccatcat ctacacggcg gccgtcagcg
    13561 tgggtctgcc tttgtcactc agggatacgc tcacccaacc gacgccggca ttgccctgga
    13621 acatttacaa tttgtgtacg gaacaccagc gggtcgacga gatccggcat gttcagtatg
    13681 gtatggtctt tacccttgtg atgctcgtac tgctcttgaa ctcggtggcg atctacgccc
    13741 gggcgaagat atccaaacgg cttcgaggat gaaactgatg gcggtagcaa tgatggaatc
    13801 gatggtgacg gtgagagacc aacgtggcga tccggcaaac aaagggcttg ctcatgcccc
    13861 ggaaaccgcc cacgtgattg ccgagcggtt ttccgttttc tacggctgca acgaggcggt
    13921 caaaaaggtc acaatggcca tccccgccgg gaaggtgacc gccatcatcg ggccgagcgg
    13981 ctgcggcaag agcacgctgc tgcgggccat caaccgcatg aacgacctga ttcccggctg
    14041 ccggacggcc ggcagcctga tcctcgatgg tgaggaaatc tacggaccgc gaatcgatgt
    14101 caccgccctg cgccgccgcg tcggcatggt attccagaag cccaatcctt ttccgaagac
    14161 cgtcttcgac aatgtggcct tcgggccgcg catccacggc atccgaggcc ggacggacgt
    14221 ggcgtccatc gtcgaagaaa gcctcaggcg ggcggccttg tgggacgagg tcaaggatcg
    14281 cctgcacgaa aacgccttgg gcctctccgg cggccagcag cagcgcttgt gcatcgcccg
    14341 ggcactggcc gttcagcccg agctgctgct gatggacgag cccacttcgg cactcgatcc
    14401 tctcgccacg gaccggatcg aggacctgat cggagcactt cgcggccaat acaccgtgat
    14461 catcgtcacc cacaacatgc agcaggccgc tcgcgtgtcc gatatgaccg ctttcatgta
    14521 cgtgggtgaa ctcatcgagt tcgactcgac gtccaagctg ttcaccagcc ccagtgagca
    14581 gaagacgcag gactacatca ccgggcgatt tggatgatca catggcgacg catttcatta
    14641 atctgctgga tgaactgatg cggcggtctt tgcgaatggg cgccgccgtc gaagacatgc
    14701 tcctcgaggc ctgcgaggcc gtgacccatg ccgatcacaa ccttgcccgt cggattatcg
    14761 atcgcgacgc cgagattgat gccgaagagg tggccatcga agcggaggcc gttcgcctga
    14821 tgaccctttt ccagccgatg ggcgccgaca tgcggcggct gtgcaccatc ctcaaggtca
    14881 acaatgacct ggaacgtatc gccgattgcg gcgtcaatat cgccgagcgg gccgcccacc
    14941 tggaacccga ggcgttggtc gaggcccgca acaacctgaa agaaatctat cccgtcgtcc
    15001 gccgcatgtt gcacggggcc cttcatgctt acgcaaccct cgatcgccaa gccgccgaga
    15061 gggttgtggc cgaggatgag gtcatcgacg ccttctacgg cgatttcatc cgccacctgg
    15121 tcatcgatgc cgccaggtcg ccccaggcca tggccgccca cttggatatc ctttccatcg
    15181 ccaagaacct cgaacgtacc gccgaccatg ccgtcaacat cgccgaggac gtgatcttcc
    15241 tggtcaccgg ccagatcgtc cggcacacca ataagaaggt ttcaggatga tttttgtcgc
    15301 ggtttcggcc gtcagggacc ccaaccggta cacctttcgc acaagacggc attctgcagt
    15361 tacctgaggc gggccggctt tttgcgagca gcagctcagg agcactgcga ttcggaaaca
    15421 tgcgcgcaaa agcgcaccag ttacgacgca agaggctacg gggccacagg cctgttcgac
    15481 ggggcagata tcggtggtca agctgtgcgg cggcgaatca gcatcagacc gcccagggtc
    15541 aacaggctca tggcggccgg ctcaggaacc tgtgaataaa ccatgttatc caaacgatag
    15601 ttctgctgat gactgcccgg tcctcccact aatctgaccg aggtgaaagg agtgtcagag
    15661 atgaagcccc aaaattcgcc accgtaattg ttggaaatcg ctcccacgta aagagaaaag
    15721 tcggcgatgt agaccagcaa gctgtttcca gagccgccgg ggccgcccaa ggcccagtct
    15781 ccgccatagg cggcgatttc ggggctgaag ctccagatgg tcataggctc attctggctt
    15841 tgagaagcca gaacatcctg gtagcattcc tccgcagggt tgatgtgccc ggattccgtg
    15901 gacacaaaac tgactccgtc gttcagttga ctgtcggcga aatcctcgct caggaactgg
    15961 ccgcccaagg cgctctccca ttgcgtcttg tccgtgtaga ttgtaaccgt caccgccctg
    16021 accgcagatg cccaagacat gcacatggcc aacgccccgc ccaaaaccgc aagtttctgc
    16081 atggttcctc ctcccttacg tacagcacaa gccgggacca acatcaccaa aacaacgcgc
    16141 actatcttac caattctccc aaggaaccac aatcgcaaat ctgctttttg tcgctgggtg
    16201 gcagatccgg ttaaggaaga caccccacca gaaaagcggc agggaggttt cgtatcccat
    16261 tacggataaa gagttactga tccaagaaga cgcggcagct cagtccatcc cggcgacgga
    16321 caacgaagcc ggatgggtgg cccctgaatg atcagccggc gtgagatcgc tactcgatct
    16381 gcccccgacg ctcaccggat tcaatgatcg tgacgatgca gtcccgggcg agcatggcat
    16441 agacggcttc gatgtcctgc tttcccaggc gaacacatcc cagagaagca tcagtgccga
    16501 tgcttgcctc atcgttggtg ccgtggatgc cgtagccgcg ccggccgacg gcgttccctt
    16561 cgattccttt cagggcaatc cagtacccgc cgagcgggtt gtccggatcg tgcgagccgt
    16621 accgcctgcc gtttcgcgga tcgacccacg acgggtgtcg cagacgggta gtcacgaccc
    16681 atttgcccgt cggcgtcttg ttgtcggtgc cgagggcaac cccgcacgtg cgcacgtaca
    16741 catcctgcag gtacaaatgc atttcatggt cgctcttgac gaccgtggcg ctgaaggggc
    16801 ctcgaacgag cttgatttga tggccggggc gaaggacgct tccggccgag agccggttga
    16861 tgctgctgat cagttcctca gtcaaaccat gcttgcgggc aatcctggag agggtgtccc
    16921 ctttgacgat gcgataactg acgaccagcg gatcgcccgg cagaatctca ggcgagaaga
    16981 tcgtcctgtc ggcgatcagg ttcagaatct cacgaacccg gcgttgtcgc tcggacggat
    17041 aaccttcccg gagaacgcgg ttcagatggt atctcgcccg caccagatct ccctgttccc
    17101 acgccagcat gccttcctgg caggcagtgt ccaacgggtc gggcggcgga ggctgggcag
    17161 gttgcgtggc aatcgccggc gcggattgca caaccggcgg tgccggcgct gcccagggtc
    17221 cttcgacggg agcggtggcg gggaacgagg tgatcgacgg tgatgagacc gggacctcgg
    17281 gtgcctgaag cggccgctca tcctccttct caaatcccgg caccgaacaa cccgcaatag
    17341 ccagccacgc cgtcaccaca gaaaggccag aaccggcgac tacacgcctt cgctgaagtc
    17401 ttcgcacgtc aatcctccct gttggcatgc ggtgtcatcg gttcagggcc tacgcggaca
    17461 ggtaatcaaa cacgcggatc ctcgcccagc gggccttgtg acgcttaata tactccaggt
    17521 gcaacgggtg gtcggcgtaa acgtcgtgcc cggccttgtc gtcgaagagg atgaccagac
    17581 cgagatcgaa gtcggtgacg ctgacttccc gacacatggt ttcgtcgcgc cggccggtct
    17641 ccagccgccg gacggtcgga atcctggcca gcaactcccg gccaccgacg acctgggcgt
    17701 cgatttggga ttggggggtg ccgggcttga gggtaaagaa cacgcagtga acgtaggcca
    17761 tgggcccttc tccgatggtc tggccgtctc gggccgatcg gacggccggg actgttcttg
    17821 acggcaggtt gttatacaat ctgcccgcac gaaacgccaa cgaaagacct cagacacttg
    17881 cgacgggcgg ctcggatctg aggtgccatg accacggctt tgcatgaccg tggtgttgag
    17941 caatcccgag acacgtgccc gcgcagggcc gtgggcacgg cgtccggcag agcggtaccc
    18001 gcgctacgat ggagtcctat tatgaaacac gtgatcgcac tcgcacttgc gctggccttg
    18061 aacgccgcag ccaacctgat gatgaaaatc ggctccggtc gattcaacgc ttccggcctc
    18121 aacaccgacc ccggtctgcg gtcgctggcc gcggccctga ccgccaactg ggtgctggtc
    18181 ctcggcctgc tgtgttttgc gatcaacgtg atgttctaca cctacgcgtt gcgggccgct
    18241 tttttgccgg tgagcatggc ctatcccatc atggtcgggg gcggctttgc gatcatcgcc
    18301 ctcgtcgcct ggtggttcct cggcgaacgt atgtcagtcg tccaatggac gggcgtcgtg
    18361 atgatcctgc tgggcgtctt cctggtggct cgtgaggtcc gggtcgatag tggggtatag
    18421 aaaggacaac cgacggatgc gatggttcaa aaagacaatt ccctggcgcg gcgaggttgt
    18481 gttccgcggc tccctgcaac cgcgggccgc agactttgaa tatcttgaga agcaccggat
    18541 ccgcatccga ccgggcaacg gagacccaga attcctctgg tcgttgacgc tcgagcatcc
    18601 tgactggggt accgccacat tgcagtgtcc ccgcgagacg caccggattg acccggcgat
    18661 tctggatcat gactggctac tgtcggagac cgagcggcag gaagcccggc tgggcgaatc
    18721 aaccgtcctg ctcggtatgc cttcggccaa gggccacatg ttgcgggacc gaaagctggc
    18781 cctgcgattc atgcgggcga tcatgggaga cgacggagtg ctaggcttgg attgtctggg
    18841 ctggagaacg tggacacgaa ccggtcttga cgaggaactg gctcacgacg ccgacctgga
    18901 cgtcgaagcc attcattcgc tgcattgggt gaagactgaa tccggcgaag aggcaggttg
    18961 gctgcattct cacggactcg ctgaaatcgg gggcttcgac tttgacatac tccgtcctgc
    19021 aaaggaactg gccacgtcgg cctgcgacgc ccttcgggcg attgcctttg cgattctcga
    19081 agagcgagtc tcgacgtcaa caacccgctt ttcgctggcg atgccgggcg gagacgtcca
    19141 gttcgtcgac gtcgacagat tcaacgccct cgcctccgcc gccgaccgcg ccttgcgaac
    19201 ggccgatgat ccggctcatt cgcgaaagcg ggccgtgtta tgcgatccgg ccgggggatt
    19261 cttctcgcga ttctccagga aggtccggcc ttcttcatat ctggccggtc cgatcgacga
    19321 tcgcgcagtg atctgttttt ccggctccgc caccgatctg atggcggaac gtgccagaaa
    19381 gacctatccc gtcttgcgcg ccttgttgga tgagcttgcc gaattcgagt ttcccgttgg
    19441 cgccaagatc ggatatccga ccgatcaccg aaccggaccc gatgaccgcg agcatctttg
    19501 gttcagcatc catcgatgtt ttgacgacaa gatcgacgcg acgctcgaga actcgccttt
    19561 ggatatcgcc gggatgaacg ccggcgatcg cgccgagcat cccgtcgagc ttctgaccga
    19621 ctggatcatc atgaccccgt tcggtcagat caacccgcgg agcatggtgg tcgtgcgcaa
    19681 gattcgcgag catcgcgagg agctgcgggc catcatggcc gagtatcgaa ggcaatcatc
    19741 caaggcctga ccatttgacc tcgtacaccc ggtagttcgt cgccctcatg ccgagcttct
    19801 cgtatgcggc catggcccga tggttgtcac ggtgcacata gagccggagg ccgcagatgc
    19861 cgggatcttg ctgagcgagc gattcgacat ggcggtagag gcggcgaaag atcccccggc
    19921 cgcggtaatc cggatgtaca tagacgctct ggatccacca gaacaccctc gcacgccaat
    19981 cgctccattc gcgggtgatc atgagctgac cgatgacccg cccgtcgcac tcggccacga
    20041 aataccgacc ctgctgcgga tgagcgacgg catgcgccac ccctcgtcgg gccgtttcgg
    20101 ggtccagccg cagcccttcc gattcaagcg cctgagcgat gatgaactct gtgatcaccg
    20161 gaacgtcgac ggctcccgcg gctcgaatga tcaagttgtc atccatgtgc ggcaatcata
    20221 tgaggaaggc ttgtgccggt ctacgctccg ccgcgggctg ccattcccaa ggcgggccgc
    20281 ctttttccgg gccgcaaccc aagcggactg ggatccggaa acatgcgcgc aagccgcacc
    20341 gttttctggc cgtaaggttt tattctctag tcgccgcccg tagttcgcgc gtgcaggctt
    20401 cgatagcctg acggccacgg tgccacgacc ggtagctgtg aaccgagagc caccccacga
    20461 gtgccacaaa gagggtgccg aagcctgcca acaaggatcg cacgacgcgc cggtcccccg
    20521 gctcgcccgg atcgatgccg gttcccgaaa actcgatgaa caccggctcg acggcggggc
    20581 cggccggggg ctcttgctct tggacatccg ccagcgaacg caacgccgcg gcgcgttcat
    20641 cctgaagcct gcgccactcc gccagctccg cctgccgctg atccatgcgc cgcacaagcg
    20701 ctcgtacgtt ctcaacatgc ccgcgaatct cccgatccag atcctccgcc cgttgcgtca
    20761 actgctcgcg gcgctgcctg atggtttgct cgcgatcttg cttaagatcg tcgagcattt
    20821 tcgctcgcaa cgaggcctcg atctcggttc gtctgtcatt cacctggcgg cgcaagcccg
    20881 cgacctgctg cacgatcgtt gccagatcga ggttcccggt caacgtggcc gagcgggcca
    20941 acctgacaat ctcctgctgc gcatcgacga ccggttggag gtccttggtc agagcccggc
    21001 caagaaccaa tcccttggta ggttcgtccg tgtcctggct gatggcgtcc agtcgctgtc
    21061 gaaactggat cagcacagcc gatccggcgt cgataaactg gcgcgcggcc cgatccagtt
    21121 ccgtctgaca ggtcagcaga tcggcctcgc ccgccagggc atctcgctgc gcagcccagt
    21181 cactcgacat cgctgtggcg gcctcgttcc acgcggccag ggcttgcttg aggtccgaga
    21241 gcgactcccg aatccttgca ccgtactcct ccttcatcgc ccgatcgatg cgggccgtgc
    21301 cgtccgcgat tgcccgttcg agtgtcctga acgtaccgtc gctgccgtcg gtgatgcgtt
    21361 tcagcgcctg gctcaactcg gcctcgcgct gctgcagcgc ctggatttcc gaactcagtt
    21421 tcctgtcttc tgtgaccgcc gcctggatct gacgggcgtc aatccggacg gatgcaggat
    21481 cgatctccgc tccggtctcg gcattcgaca acgaagccag ggccgcctca acctcccgac
    21541 gttgtgcttc cgccgcagtc cattgagcca ccaggcgagc tccttcctct tgttctgcga
    21601 acgcaggacg agtcgcaatt tccagacgat gctcgatctc ggcgatctgt tgccggattc
    21661 tctctcgctg aggtgttgac gagggccgcg aggcgggcgc cgaagccggg ctccggtaca
    21721 acgcgaccat atgccgcgcc gtttggttca ttcgggcggc agcctgatcc ggctcccggg
    21781 tttgcagccg aaaggtgagc aacccgcgcg gcacgtccat ctcctgaatc ggcagaccgt
    21841 ctgccttggc aagatcggca catgccgaca gcgtgacggc atctccaggc cgggtcttga
    21901 tgtggccgtt gaccgtgtag agcggcttgt atgtggcatg cgaccacaca ccgagccccg
    21961 ccgaaaccag gacggcgatg attccaaaag cgaccaacgc cgccgaagac tccgacctgc
    22021 cgggcttgtc aatggttgcc gcctcgcgat cgggctgcga cgtccccgcg aaaaacgtcg
    22081 tcggttgggc gggcacggcg gattcagtct ccatggtgtt cttcagaacc gcgatttgct
    22141 tttccagccc ggccgcccgt cggctttgga tttctgcctc ctcaaccgcc tcggcaagcc
    22201 gcttccgagt ctcgtcgcga tcccgtccaa gccgcgaagc ctgttctcgc agttcctgat
    22261 tcttggcggc gaaaactctg agttgctctt cccggtcttt gagcagtgct tcctgtttcg
    22321 ccagcagctt ggcagcctgc tccagcttgg cctgcgcctc catctcgcga ccatgttgtg
    22381 acgaccatgc cgcctgttcc ttccgaagcc gggcctgctc gtcacgaagt ctcgactcgg
    22441 cttcagccag cttgcgctct cgctccgtca gttcccgctc gatctgatca agcttccggc
    22501 ggacatcgcc aagcaagccg tgtgccgatg agtcgtggtc ggcgggcctt ccttcagact
    22561 gcggatcggg tcttcttgcc acggcggacc tgccctcggg gtactcgggg ggccatcatt
    22621 cccttgccat gtcgcgaacc aacccagtct atccttgccg gcaggcctca acaagcgagt
    22681 cctccgcaga tgcaggcgaa ggggggcgtg acagaagcgg cggcacttct ggcggctgac
    22741 ccggttgtcg tgaacgcgag ccgaaagcag agcacttcag cggtctttcc cgaagggcaa
    22801 acaggccggt ccttttacga ctggttcacg gtcggccaat tcctccttca cggtgtcacc
    22861 ccatcgaagc cttttcagca ggcttgtcct tgtcgagtaa gcccgttgaa aagggctgag
    22921 accatagaaa ggaaaaagaa ccgcgggcga ccaccgaccg gccgtatgag ggccggcctg
    22981 tcagcccgcc tttggcggga agaccccctg aagatgttga tcgcccaacc tggaccggtc
    23041 ggcatgcccg cccagactgt cacgcacccg gttggccacg gcgaggaaga cgggaattgg
    23101 acaaaagcgg gtggatgcgt atgctatcgg cctttcggcc ggggtactgt gtgctggtgt
    23161 ccccgatgcc gtcgggcaaa ggactatgat cattgcgctc aatctcttcg acatcattcc
    23221 cggcaaggaa gccgtctacg ccgagtacct tcgccgcgtg cgtccgatcc tggaaaggca
    23281 ccatgctcgc gtcctgtttt atggtcggac gcgggcacga ttcatgggtg cctgcaatca
    23341 agaatactgc ggcgtcatcg cctacgaaga tcccacggac ctgacccgat tctcacacga
    23401 cccggagttc gcggaaatca aaccactgcg cgacgcctca actcacaact atgttctgac
    23461 ggtcgccgag gagaccggca tcgaggatgc ggccgagatg ctcagcagat cttgacggga
    23521 aaaggcatca cggtgcaggg acccggcgac tgccggtgga ccggttgggc tttcggtcgg
    23581 cgggggttgc caaaggccgg gtgccgatct atacttcgtt agatgacgaa gtataagctc
    23641 tggcggatac ggccatgcag gagtatctgg cgatcacaaa agccctgggt gacgagacgc
    23701 gagtgcgggc cctgctggcc cttcggggcg gcgaactctg cctttgccac atcatccagt
    23761 tgttgcggct tgctccggcg acgctgtcca aacacatgga tctgctggtt caagccgggc
    23821 tggtcgagcg gcgcaaggac ggccgatggt gctacttccg actgattggt gccaacgcgc
    23881 aaccggcggc gcggcgggcg ctgagatgga ccttggatgc tctggcggat gatcccgtca
    23941 ttcgtcgaga tgccgaggca ctgaacaaga tcagatgtgc cgatcccaag gagctgagtg
    24001 catgctacag aagcggaaag tactgattct gtgcacgggc aattccgctc gatctcaaat
    24061 ggccgaggga ttctggcgtc acttgggcgg cgaccgttgg gaggtgttca gtgcgggcgt
    24121 tgatcccaag ggagtcaatc cggtcgctgt tgcggtcatg gccgaggccg gcgtggacat
    24181 cagcgctcac ccattagccc ccgtcttgtc aagcgcgaaa actcccttgg cgaatctttc
    24241 t
//
LOCUS       DAOTPW010000062        23924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2194_length_23924_cov_5.113788, whole genome shotgun sequence.
ACCESSION   DAOTPW010000062 DAOTPW010000000
VERSION     DAOTPW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23924
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2194_length_23924_cov_5.113788"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="P5316_07575"
     CDS             <1..278
                     /locus_tag="P5316_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84855.1"
                     /translation="PQGSGTYNYDRLVGMTYPTRPETGARSLWEIGGHYTYFDEIGAV
                     FRCKLRRSARQPAVHTSGHRRPVPPRGAVTRAHAGQQLQVDHGTNLR"
     gene            complement(995..1069)
                     /locus_tag="P5316_07580"
     tRNA            complement(995..1069)
                     /locus_tag="P5316_07580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Pro,seq:cgg)
     gene            1954..3666
                     /locus_tag="P5316_07585"
     CDS             1954..3666
                     /locus_tag="P5316_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84856.1"
                     /translation="MLVFGVNRVGNPAPLYNPAGYDEALYQRIREAGGTCVRLTASPR
                     DIEAERGKRDWREFDRDVALALKYGQEPIVCIVNTPAWASPDDQPTHLHPYRTELLPE
                     FGDFCTDLARRTKGKVKYFQLWNEQNGCGWYFFEGFNRADEYLPVLKVCYDALKKGNP
                     DCILSMGSLDDAEGHAPIFVRKIYEEQKKQNISGPLFDVISDHPYSDTPQIMRAKLDA
                     LRWLLKANGDGDKPFWLTEYGWHTGNTPPAEQASRLAAVLEAFISPGWSDVQAAVYLC
                     IADFENHATGFGLVDTNLRPRPSFHAFQGASRFGAYPPCEIKADFSAADRLVITWRTL
                     QPTRGKVMLQGPDSSTAVTRTVTEPATEQRAEFDGIQPDKVYRYSIETVCERGGRAKS
                     IKSADYEIRSPGPAVFNGDFDEGFFAGIAKGWRIDGEGFCTDAALIPLTHLVYGKHAQ
                     VVYAAGERGHAALDSTLSTLVAVERGQEVRISVSWFGNATADSVKVLSRLGIGPEGQS
                     QPGAVGVQWSSWEETPRFWDDASLSAKAANSVIRVFVQCRSEGSLEKATATFMLDNVR
                     IEAE"
     gene            complement(3701..4108)
                     /locus_tag="P5316_07590"
     CDS             complement(3701..4108)
                     /locus_tag="P5316_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRR84857.1"
                     /translation="MRGCRWTASVAVRRWIFLAYWFTMFAGTHTPRLEIIRPEVFRIP
                     HADKVIHFCMFAGWMSLCWWLLRSPRGRPGRRAVTGAFLAGALYAAFDELTQSLVGRD
                     TSFGDFLADIAGMLAALWVLEAWHRRQTSRKTA"
     gene            complement(4119..5123)
                     /locus_tag="P5316_07595"
     CDS             complement(4119..5123)
                     /locus_tag="P5316_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRR84858.1"
                     /translation="MNKTSGTKTRQFIKDLTAGAFLEDQVFLISAKDLRTTSNGSLYI
                     HCVLADKTGQLLGRIWQATEPMFEQMPEGGFMRFKGRVENYKGSLQFIVDGMRPVDPK
                     SVDLSEFIPHTQEDVAAMFARVKEILRQVKNKHLLNLAKQFILDEPLMERFRQSPAAM
                     QMHHAYVGGLLEHTRNVLELALLVMPRYPAVSLDLVLVGIFLHDIGKTAELRCETSFQ
                     YTNEGQLVGHIVQAAIWVQEKAKAVEAETGEPFPADVKNALQHIILAHHGMYEFGSPK
                     LPMMPEAVAVHYLDNLDAKLNMFMTRIGADPDPASDWTEYVRGLDVKIFKKDVMGIRR
                     "
     gene            5335..7110
                     /locus_tag="P5316_07600"
     CDS             5335..7110
                     /locus_tag="P5316_07600"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRR84859.1"
                     /translation="MLWSQYFIPTVKESPADAVAASHVLAIRAGLVRQVAAGSYTYLP
                     LGYRCLRKIEEIIREEMNAAGAIELHMPALQPLSLWEETGRAEAMGEVLMRIKGSGWQ
                     TGVVLGPTHEEVITETVRAYISSYKQLPINLYQIQTKFRGEERAKSGILRTREFLMKD
                     AYSFDVDLAGLDASYRRMYDAYCRIFKRCGLPYLAVEAESGPIGGDASHEFMVLTDAG
                     EDFVAISEDGSYAANIERAARAVPPLPGDPAPGDMKSLETVHTPGCPGIEDVCRFLGV
                     QSSQMIKTLVYRFEPSSSEAKAGAGYVIICVRGDHDVNENKAKRLCPGGTLTMGEIAR
                     AEQDGFAIGYVGPHVVNQKDCTLIIDPDARALVNAVTGANKKDHHVTGFNWVRDVAAE
                     KLSAAIVADVRNVVEGDIAPNGSGSRMLFRKAIEVGHVFKLGTKYSASMGATFLDENG
                     KAREFIMGCYGIGVNRIMAAAIEAHHDADGICWPMSIAPFEVLIVALDTREEGVMSAA
                     RRIHDRLAEEGMDVLLDDRDARPGFKFKDADLIGIPIRVTVGKKGLTEGVVEVKMRDS
                     KEMVKLTPEAAVAEVMNWVKEAKVL"
     gene            complement(7135..7848)
                     /locus_tag="P5316_07605"
     CDS             complement(7135..7848)
                     /locus_tag="P5316_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84860.1"
                     /translation="MIRVPVTHIQELSQAFLERYLDEREKGPFESVDDFVRRCRPTES
                     EGRLLLDSGALDVFGISRPMLFWRLRRLQGMGHEHGGTLWKTQPEKDDQTPPVDLDCP
                     DAWQVARREMDLLGFPITIDPITYLSRDDKGRTIDWSRYVPVSELNEHTGRRVSVCGL
                     MVADRVNRTNTGDLMKFVTLADRTGFVETCLFPNTYRRFGHLTAANPILAVDGVVEMF
                     ENKRGLTLRAERVQVPAMI"
     assembly_gap    8109..8208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8209..11056)
                     /gene="dnaE"
                     /locus_tag="P5316_07610"
     CDS             complement(<8209..11056)
                     /gene="dnaE"
                     /locus_tag="P5316_07610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRR84861.1"
                     /translation="MPIPLHNRSSYSFGSSLTRPEELAAFAAEHGIPAIALTDLNGLY
                     AAVEFQKACQRAGVKPIFGAELTLTSHGVDPRGLPSLPTVNDRENRQHEPAASSRGVY
                     PRGSENQVRSAPADHTIVHRSPFTIHHSTFNIQHVRSAPADHTAERITVTLLAENPTG
                     YGNLCRLISLHHLRETPLDLQDLCEHAEGLICLLGQWNEPRGLSPWYTPILRDAFEGQ
                     LYVELAVHGPDDVLVARRRARIADELGIPVLASCESRCLKRWHMPALRALASIGTLTL
                     LDEPHPDKPQGTWHLRTPSEMKHLFHRRPDALANTMKVAERCNMTIDLSQNRFPPFES
                     SDRRSAAQHLRDLCVAGCRRRYVEQPQQKGIGGKRPTLAEALERLDRELTVIEQVHYA
                     EYFLVFHEIVEYCRSWGIAVLARGSAADSLVCYSLGVSHACPFRFDLPFDRFLNPERA
                     RFSKMADIDLDLPWDRRDEVVRWVYERWGYDRVAMIGSPNRFHGRAVVAELGKVLGLP
                     PHEVHEITRYVPHTSADDLARAIPASPEARRLSLEEEPLPSILRVAGLFEGLPRHWSM
                     HPCGLVVSPEPLTDLVPVQRSPKGPLVAQYDMDAIEELGFIKIDLLGQAGLSVLRDTV
                     DEVHRTEGITVDLEQDVDYSEGQTWDMIARGDARGVHHIESPAMTSLLVQSNCRDIDC
                     LTAIVAIIRPGAANQGKKEAFCRRYQQLEPPGQVHPALTSVLAGTYGLMIFEEHILQA
                     AVEFAGMNLGRADVLRRALNKENLPMIRRMEGEFREAARLQGRSEAEINEVWAMIEGF
                     RGFMFNKAHSAEYAVEAFQGAWLKRRWPAHYLAAVLSNYRGFYAHSPTLPQILYVMEA
                     LRLGVGFLPPCVNRSRKRFSVEHVQVPHRAGGEGPNSNESRPALTSHGVYPRGLPFLP
                     TGYGPEDRPDNPAGSTRGVYPRGLPFLPTGYGPED"
     gene            complement(11070..12290)
                     /locus_tag="P5316_07615"
     CDS             complement(11070..12290)
                     /locus_tag="P5316_07615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRR84862.1"
                     /translation="MFLLHFDADAFFASVEQAADNRLRGRPVAVGGLHRGIVASASYE
                     ARQYGVRTPMPMSQARRLCPKLIVLPVRFELYEQFSENIFGMAEEITPIVEKQGIDEG
                     YLDLTGTPACRKREPAEVADKFGRDVRDWLKVTISRGVARTKLISQIASKLNKPNGLT
                     VIPPDESTELSFLQPLAVKWLPGIGEVGARLFESAGILRIGQVAEMPLDWLTELVGSA
                     AHQIRDFARNIDPRPVELTRPDAFSYGHQETFPEDKTNPNQVLAALCRLADEAFLRLR
                     ADGKQARTVTVKIRYTDMDEHQGQMSLPEPTDVEMQVYPLLSKLLDRLWDRRVRIRMV
                     QVKLSNIYSGFSQMDLFGAKQKQRNLAIACQAIRERFGTKAMMRKHDWEAVSDQHSAV
                     SKDMLTAGCLAGLE"
     gene            complement(12325..12684)
                     /locus_tag="P5316_07620"
     CDS             complement(12325..12684)
                     /locus_tag="P5316_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRR84863.1"
                     /translation="MQDFRKLQVWQKGHQLVLAVYRDTRRFPKEEIFALTNQMRRSAI
                     STCANIAEGCGRQTNSELSHFMHIAMGSACELEYHLLLARDLGMLAIDRYKQLATDLA
                     EVKRMLTSLIQKVKAKN"
     gene            complement(12731..13204)
                     /locus_tag="P5316_07625"
     CDS             complement(12731..13204)
                     /locus_tag="P5316_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRR84864.1"
                     /translation="MRTFIDITDLCTKLYDHNEAAGGQAGNALVNNGGIDESDLVSIA
                     AGWPGELAEGCARLDPAAILRATPRSFALRVRGQSMVNAGIYDGDIVVGEITPVARPG
                     AIVVALIDGESTLKRLMVERGRPYLISENPTCPAFMPLGELVIQGVVHTVVRRVQ"
     gene            13324..14319
                     /locus_tag="P5316_07630"
     CDS             13324..14319
                     /locus_tag="P5316_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRR84865.1"
                     /translation="MPGEQLGFGWEETPAAPVPPARATMLSQRLARLARRGIYLGTSS
                     WKYPGWLGQIYTPARYETRGRFSEKRFNETCLEEYSRVFPTVCGDFAFYQFPSEKTWH
                     EIFAQVPRGFRFSLKVPEDITVERFPDLRRYGQRAGQRNPHFMDASVVKSELLDRLEP
                     YRDKLGILIFEFGTIREGPMVDPKGFAAALDRMLSRLPLDHFRFSVEVRNPEYLEFGA
                     DYLSCLRGHGVAHCYNSWTRMPSLPRQIELPGTLTAPFATARLLLRPGRTYQQAVDRF
                     APYEKVQDPYPEGRSALARLMETCVAENRELFAFVNNRFEGNAVETIEGVIERFE"
     gene            complement(14358..15620)
                     /locus_tag="P5316_07635"
     CDS             complement(14358..15620)
                     /locus_tag="P5316_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR84866.1"
                     /translation="MRTVLAIAGKNLLLCWRDRLAFFWWMIGFPLLIAILIGTMFAGV
                     LSGQSQPVLVAVIDEARNSDSREFIAALSQSEILQISRSSRDDAETCVRRGSLSAFVL
                     LPAGFKLTPAGLLDARLPIAVGADPSRTAELAYLQAALREAAAGVIRARWNDPLRRSE
                     MIDAWLKAQGRSGPSRSLDRAAVEALLATFDPRLTSMPESAGPRPALSNVELIPFTAW
                     RNTPRSPFDVCFPLGMIWGLLGLTAEFAMAFAKERETGTWLRLRIAPIRRWQILLGNG
                     LACLVASVGVIAMLLLVGRVACGVRVDNPLALLLGVPCVALCFVGLTMLLSVLGRTES
                     SVGGAGWACLLVMAMLGGGMVPRMFMPSWMETAGNLSPVKWAIVAFEGGIWRGFSLRE
                     ILLPCSLLLAQGLVYGTLGVIIMPRFKE"
     gene            complement(15620..16510)
                     /locus_tag="P5316_07640"
     CDS             complement(15620..16510)
                     /locus_tag="P5316_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR84867.1"
                     /translation="MIRVDHLRKTFGSTRAVDGISFEVRRGETFGLLGPNGAGKTTTL
                     HMLVGLLKPDAGDIHIDGATDPTRPRVRRSIGLAPQTLSLYEDMTATENLKFFGRLYG
                     LRDGRLSERVAQAIEQAGLADRSRHLVKTYSGGMKRRLHLACALLHQPQVLLLDEPTL
                     GVDPQARLHILEDIEQLGRQGRTILFTTHYIEEAQKLCNRVAIMDHGRILAMDTVSAM
                     IDCHGGQAVVHAELDEAPPGDWSLPGRLNGTSLSAETDHPFETVRRWAASGIRIRSLS
                     IERPSLETVFLRLTGRRLRD"
     gene            16780..18210
                     /locus_tag="P5316_07645"
     CDS             16780..18210
                     /locus_tag="P5316_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRR84868.1"
                     /translation="MWTFMCRRAGRAFAAVGGMGVLMAANLGCFPEKSFKMDPEASVR
                     VSERLVNGEDGASTRVWLRRTQRELAGLLPDSVASPLLTEELVNGDGRAVDVLRHFGV
                     SRQSLQSIVKNYRGIRYSAQAASRDYCIEKQAAAWPGFEDVWIPMRLAVDDQLELSGR
                     LGYARNGNGEIIEADCVVILPGLFGDHGVKRSRDLAIPLRESGFHVLHLELRGHGQTE
                     SRYPRMYHTFGVMETDDLMQVSDYLEQLPHIRRTGLIAYCWNASIALLAAWYDGRLPD
                     DPQISPAIRPYLQCPPEPLRRRFSAGIIAFSPVVRWEVLMDELDRQRSIWAHPIYAAI
                     QDTIRDRMVRKGYPERSGSLRNLIEYEHNGYGIPMPHGAREGYPFLRLISYNGEPSGD
                     KLEFARMPVLIVHGADDPLVPAQDVADLMATVDNPRVGAIILPSGGHVGFAAYCPRYY
                     LSLITNFFDPVNGPAGRGAAISAAAR"
     gene            18668..19033
                     /locus_tag="P5316_07650"
     CDS             18668..19033
                     /locus_tag="P5316_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRR84869.1"
                     /translation="MNAKLTTKRSPENDCSTHANVSRGKSDQSPLSGPKLKESLERLE
                     RQLVIEALIRTKGNQSRAARSLGITERIMGLRVKKYGIDPQSIRDAYQSDPIVQQCSS
                     AAALRTMSNEEKDHVRSSR"
     gene            19075..20508
                     /locus_tag="P5316_07655"
     CDS             19075..20508
                     /locus_tag="P5316_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRR84870.1"
                     /translation="MNENTFKRLMGKLLPLLAVLATAILATRAFGQETQPPSASDILD
                     KVSLLSVDVDTLWVLITAFLVFWMQAGFALVETGLTRAKNTVNICMKNLLDFCFASIG
                     FWAIGFAIMFGTGNKWFGTSGWFLQGDAGTTFAALSWTSVSLDAKFIFQLVFAGTAAT
                     IVSGAMAERTRFTSYMLYSLAITVFIYPVAGHWIWGGGLLSAGNMFGEGKGMFDFAGS
                     TVVHSVGGWLALTGAIVLGPRMGKYGPGGKVNAIPGHNFPLAILGVFILWLGWFGFNP
                     GSTMMATGCGAAIAHIAVTTNFAAATGTIGALLTSKLWFSKWDASMAGNGCLAGLVAV
                     TAPCAFVDTWAAGLIGLVAGVLVVGSVVFIDRLLKVDDPVGAVSVHLVNGIWGTLALG
                     LFSNPVHGSTGPLPGLFYGGGVEQLKMQLIGAVLVGIWCLLAGFALFLGLKALTGLRV
                     SPEEELRGLDVDEHGMEGYPNFQLLKH"
     gene            20647..20985
                     /locus_tag="P5316_07660"
     CDS             20647..20985
                     /locus_tag="P5316_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRR84871.1"
                     /translation="MKKIEAIIKPFKLDEVKTALTAIGIQGLTVSEVRGFGRQKGHTE
                     HYRGAEYTVDFIPKVKIEVVVPEAKVAEVLETIAKAARTGQIGDGKIFVSSLDEIVRI
                     RTGEKGPAAI"
     gene            21231..23864
                     /gene="glnD"
                     /locus_tag="P5316_07665"
     CDS             21231..23864
                     /gene="glnD"
                     /locus_tag="P5316_07665"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRR84872.1"
                     /translation="MSNHPLPTTDSESDPSRLKEHFILGRERIRHMYHSGGSAAEVSK
                     ALTALCDSVVRQAYLAAIQDLPPDDRVSVLGSLSLVAVGGYGRGDLAPYSDLDLLFLT
                     VSQPSAVTRDFVSRLVRDLWDAGLHLSQSVRSPGECGSFAETDFAARTALFETRLIVG
                     DPELYTNLRLRVCPPRGHRALRAFINQAVASRSEEHEDYFSRTALLLEPNVKKSPGGL
                     RDVHLLRWLAMARYGDSDLNRLRADGILAAEEADTIVGAHEFLRGIRHALHFHAGSAQ
                     DVLTREEQLRLAEQYGYRQQGALLPVECFMQQYYRHTAGLRDLLLRFIERLERRSPVR
                     RLYSRILSRRVTPSIVAYPDHIEIVGGTDGRSFGQADTVLQAFDLARRFGVPVHSDTL
                     SEIRQHLPACIVTTAERRRFLECLAQPHGLGTLLRNLHGVGLLQRLIPPFEHARGLIQ
                     FSLLHHYTIDEHVIRAVEAAARLADERTPLGLAYRDIRRKDLLHLALLLHDLGKGLGE
                     DHCEVGRQMAEDAARDFDLDEHEARLLVFLVRRHLLMAHTSQRRDPADADTLLQFSRL
                     VATPELLRMLYVLTAADTEAVAPGEFTTWKESLLTNLYARAMECLTGETPLGDAANRL
                     AAVRSSLHERLAGTFDPTWLTGQLDAMPPSYLTGTEPRRVKAHLRLLHGMDGAGVRVT
                     AEYRKDLQLSEYTVLTHERLIPGIFSKAAGALAASGFQIVDAQIITRPDGVVLDTFRG
                     LDLDFSGPPPPERMTEIGERIERVLLGQETVEALITRRGEPLSRGPHAGPQEPAQVEI
                     DNSSSDRFTIIEIFANDRLGLLYAITRTMFEIDLSVHSAKISTHYSQVVDAFYVTTNN
                     GSKIDDPALIRRIRQRLLEVV"
BASE COUNT         4639 a         6953 c         7562 g         4670 t
ORIGIN      
        1 gcccccaggg ctcgggcacc tacaactacg atcggctggt cggcatgacc taccccacac
       61 ggcccgagac tggtgcccgc agtctgtggg aaatcggggg acactatacg tatttcgatg
      121 agataggggc tgttttccga tgcaaactgc gaagatcggc tcgacaaccc gccgtccaca
      181 ccagcggaca ccgtcgaccc gtgccgccac gaggtgccgt gacccgggcg catgccggtc
      241 agcaattgca ggtcgatcat ggcacgaact tgcgctgaga cacgatctcg aactgcagca
      301 acagcccgca atggagcagg cccgactggc tggcgtcgct tctgtcgcag gggctcgatt
      361 gtcttcggtc gttggtagac ctcgatcggg aacagctccc gtgccacagc ccatcgatac
      421 atcgcaatga tgatcgtcgt atgtcgtgcg gccgcgtccg acgatatggt atgagcgatt
      481 gtagcacacc agtcacgcag tgcggccgga ccgatcgtta ctgcaggcat gcgaccgaaa
      541 tgttctcgcc agcgtcgcag cgcgatcctc aacatggact cgtacgagtg actgtactgt
      601 ccttggaggg cttgccaata ttcggccacc aattgggttc ccgtccgtcc gtcggctgga
      661 cgaattgcgc ctgtcggctg cggcagagat tgccgtgttt cccactcggc caacaaccgg
      721 gcgtagcgcg cgcggctctc gggtgtgcca tattcgccga gccaatagtc gcgacgacga
      781 cctgtggctg aatctcgtaa ggtgacataa gcgattggtt tgccccgcac cacccgtctc
      841 cggtagctcg gaggcctttg agaatgacgt ggcatatgcg gctccttgct cgtttgcgcg
      901 gagcgcgctc cgcgcaaaaa cccttagttt gccgcactga accacgaatg gcaggtatgc
      961 gtaagtcacg cattcggcgg gagttatcca ccaatcgggg cgactggatt tgaaccagcg
     1021 acctcttggt cccgaaccaa gcgctctagc caggctgagc tacgccccgg tcagttattg
     1081 atattatcct gcggcgggcg aaaagcaagc cgtgcgtacc cgtgcgtacc gagcatagcc
     1141 ggcaagggtc cgatgggctt tcggcagctt tttcagagcg gcgtgcgatg ggcaagggca
     1201 aggatgggca acgcggatga ccggctggaa gcgtgttcca gagtgccggc caatgacagg
     1261 ctggaagctt gtcccacacg gcgtgacaga atggaaatcc gtcccacaaa gcctgtggca
     1321 gactggaagc gtgtcccaca gtgtcggccg acgacaggct aaaagctcat cccgcaaggc
     1381 aaggcacctg tttggcaacg tggtgcgagt tgcggggcct ccgaggtcct gaaaggttgt
     1441 ttccgcggcc ctttggcggc gcccctgcgg actggtagaa tactgtcggt ttgcgggaag
     1501 gcggagataa gaacatacga ttcagaggtc gttatgagtc gctgcttaaa gcctgaggcg
     1561 gaccgcaacc caggcgtact gcgagccaga aacatgcgcg ccaattgcgc accagtctcg
     1621 acggaagagg ccaaggcaaa accccgaagg cgaaccgtct tcttgcgggc cgcaagccaa
     1681 gcgcgccgcc gtgcggcaac atgcgcgcaa ggcgccctga tgtccggccg tgaggttcca
     1741 aaggcgaggg gccgcaagtc ggcggcacag aggtttcggg gcaggttctc agccgttgga
     1801 gttctgggat tgcttcttgc ggggtgccac caggctgccc gcccacccgc gcacaagcct
     1861 gcaaacgatt cgaccgaagc cggtgaagtc tctgccgtca cacggccgac cgaagcatac
     1921 gctgcgtctc aaccggcgga ggatgatgct cggatgctgg tcttcggcgt caaccgtgtg
     1981 ggcaatcccg cccctttgta caacccggcc ggctatgatg aggcgcttta ccaacgcatc
     2041 cgcgaggcgg ggggaacatg cgtgcggctg acggccagtc ctcgcgatat cgaggcggag
     2101 cgaggcaaac gggactggag ggaattcgac cgcgacgtgg ccttggcttt gaaatacggc
     2161 caggaaccca tcgtgtgcat cgtcaacacc ccggcgtggg ccagcccgga cgaccagccc
     2221 acacacctgc acccctaccg gacggaactg ctgcctgagt tcggcgattt ttgcaccgac
     2281 ctggccaggc ggaccaaggg caaggtcaag tactttcagt tgtggaatga acagaacggc
     2341 tgcgggtggt acttctttga aggcttcaac cgggcagatg agtatttgcc cgtcctcaag
     2401 gtttgctacg acgctttgaa gaagggaaac ccggattgca tcctgtccat gggatcactt
     2461 gacgacgctg agggccatgc gccgattttc gtacgcaaga tctacgaaga acagaagaaa
     2521 cagaacatct ccggaccgct ttttgacgtg atcagcgacc atccctacag cgataccccg
     2581 cagatcatgc gggccaagct ggatgccctg cgctggctgc tgaaggccaa cggcgacggg
     2641 gacaaacctt tctggctgac cgagtacggc tggcatacgg gcaacactcc gccagcggag
     2701 caggcttcgc gacttgcggc ggtgttggaa gccttcatat cgccaggctg gagcgacgta
     2761 caggcggccg tgtatctgtg cattgccgat tttgaaaacc atgcgacggg ttttggactg
     2821 gttgatacga atctgcggcc gaggccgtcc tttcatgcct ttcagggagc gtctcgcttc
     2881 ggagcctatc ccccctgcga gatcaaagcc gacttttccg ccgccgatcg gctggtaatc
     2941 acttggcgaa cactccagcc cacaaggggc aaggttatgc tgcaggggcc ggattcctcg
     3001 acagcggtca cgcgaacggt gactgagccg gcgacggagc agcgagctga gtttgacggc
     3061 attcagccgg acaaggttta tcggtacagc atcgagaccg tgtgtgaacg cggcggacga
     3121 gccaagagca tcaagtcggc cgactatgag ataaggtcgc cgggcccggc ggtgttcaat
     3181 ggggacttcg acgagggatt ctttgccggc atcgccaagg gatggaggat tgacggagag
     3241 ggattctgta cggatgctgc tctcatcccg ttgacgcatc tggtttacgg caagcacgcc
     3301 caggttgtct atgcggcggg cgaacggggc cacgcggcgc tggacagcac gttgagcact
     3361 ctggttgccg tcgaacgggg tcaagaggtc aggatcagcg tttcctggtt cgggaatgcc
     3421 acggcagatt ccgtcaaggt tttatcgcgg ctgggcatcg gccccgaggg gcagagccaa
     3481 ccgggcgccg tcggtgtgca atggtcgtcc tgggaggaga caccccgctt ctgggatgac
     3541 gcctcgcttt ccgcgaaggc cgccaacagc gtgatccgcg tgtttgttca gtgtcgctcg
     3601 gaaggttctc tggaaaaagc gaccgcgacg ttcatgctgg acaacgtgcg gatcgaggcg
     3661 gagtagaagc cggatcgccg ggcgaccgcc cggacgctgt tcaggccgtc ttgcgagagg
     3721 tttggcggcg atgccaggct tccagcaccc agagggccgc cagcattccg gcgatgtccg
     3781 cgaggaaatc gccaaatgaa gtgtcacgtc cgaccaacga ttgcgtcagc tcgtcaaaag
     3841 cggcgtaaag ggcgcctgcc aagaacgcgc ccgtgacggc cctgcggccg ggcctgcccc
     3901 gcggcgaccg cagcagccac caacacagcg acatccaacc ggcgaacatg caaaaatgta
     3961 tcactttgtc ggcatgtgga atgcggaaca cctcgggtct gatgatctca aggcgcggcg
     4021 tgtgggtccc ggcaaacatg gtgaaccaat acgccaggaa gatccagcga cgcacagcga
     4081 cacttgcggt ccatctgcaa ccgcgcatca ggtcctcgtc atcgccgtat gcccatgacg
     4141 tccttcttga agatcttcac atcgaggccg cggacgtact cggtccagtc gcttgccgga
     4201 tcggggtccg ccccgattct ggtcatgaac atgttgagct tggcgtccag gttatcgaga
     4261 tagtggaccg cgacggcctc gggcatcatg gggagcttag ggctgccgaa ttcgtacatg
     4321 ccgtggtggg cgaggatgat gtgctgcaag gcgttcttga cgtcggccgg gaacggctca
     4381 ccggtctcgg cctcgaccgc cttagctttc tcctggaccc agatcgcggc ctggacgata
     4441 tgtccgacca gttgtccctc gttggtgtac tggaagcttg tttcgcaccg cagctcagcg
     4501 gtcttgccga tgtcgtgcag gaagatgccg accagaacca agtccaagct gacggcgggg
     4561 tagcgaggca tgacgaggag ggccagttcg agcacgttgc gggtgtgctc gaggagcccg
     4621 cccacgtagg cgtgatgcat ctgcattgcg gcgggcgact gccggaagcg ctccatgagg
     4681 ggctcgtcca ggataaactg cttagcaagg ttgagcaggt gcttgttctt gacctgacgc
     4741 aggatctcct tgacacgggc aaacattgcc gcgacgtctt cctgggtgtg cgggatgaac
     4801 tcggagaggt ccacgctctt cggatcgacc ggcctcatgc cgtcgacgat gaactggagc
     4861 gaacccttgt agttttcgac tcgccccttg aatcgcatga agccgccctc gggcatctgc
     4921 tcgaacatgg gctcggtcgc ctgccagatc cgacccagga gttggccggt cttgtcggcc
     4981 aacacgcagt ggatgtacag ggaaccgttg gaggtcgtcc ggaggtcttt ggcggagatc
     5041 aggaatacct ggtcttcaag aaaggcgccg gcggtcagat ccttaatgaa ttgtcttgtt
     5101 tttgtgcctg aagtcttgtt catgtcgctc cttccatgcg gcgccgaggg ggcgaaaccg
     5161 cggcggccac gatcagcata accgctcttt ggtcatcttg ccacgtttga ggcatatccg
     5221 tcttgccgcc ggagcaggcg cagcatcggc tgccggagtc aggggcggct tgcgtccacg
     5281 gcccatgatc gttaaaaagg ttcacccctg gctcactgac attgagaggt acgcatgctc
     5341 tggtcacagt atttcattcc aaccgtcaaa gagtcgccgg cggacgccgt ggccgcatca
     5401 cacgttttgg ccattcgggc agggctggtc cggcaagtgg cggccggctc atacacctat
     5461 ttgccgctcg gataccggtg ccttcgcaag atcgaggaga tcattcgcga ggagatgaac
     5521 gccgccgggg cgatcgagct gcacatgccc gccttgcaac cgttgtcttt atgggaggag
     5581 accggccgtg ccgaggccat gggcgaggtc ctgatgcgga tcaagggcag cggttggcag
     5641 accggcgtgg tcctggggcc gacgcacgag gaggtcatca ccgagaccgt cagggcgtac
     5701 atcagcagtt acaagcagct tccgatcaac ctgtaccaga tccaaacgaa gttccgtggc
     5761 gaggaaaggg ccaagagcgg gatcctgcgc actcgtgagt ttctgatgaa ggacgcctac
     5821 tcgttcgacg tcgacctggc ggggctggat gccagctatc gcagaatgta cgacgcctac
     5881 tgtcgcatct tcaagcggtg cggtctgccg tacctggcgg ttgaggccga gtccggcccg
     5941 atcggggggg atgcctcaca cgagttcatg gtgctgaccg acgccggcga ggatttcgtg
     6001 gccatcagcg aagacgggtc ttatgcggcc aacatcgagc gtgccgcccg cgcggtgcca
     6061 ccgctgccgg gcgacccggc ccccggggac atgaaaagcc ttgagacggt tcatacgccg
     6121 ggctgcccgg gcatcgagga cgtatgcagg tttcttgggg tccaatcatc acagatgatt
     6181 aagacgttgg tgtaccgttt cgaaccctcc tcgtcggaag cgaaggccgg ggccggttac
     6241 gtcatcatct gcgtacgcgg cgaccacgac gtcaatgaga ataaggccaa gcggctctgc
     6301 ccgggcggga ccctgacgat gggcgagatc gcccgggccg agcaggacgg tttcgccatc
     6361 gggtacgtcg gaccgcacgt tgtcaaccaa aaggactgca cgctgatcat tgatccggac
     6421 gcgagagcgc tggtcaatgc ggtgaccggg gccaacaaga aggaccatca tgtgacgggc
     6481 ttcaactggg tccgagacgt agcggccgag aagctgagtg cagcgatcgt ggccgacgtc
     6541 cgtaacgtgg tcgaggggga cattgcgccc aacggcagcg ggtcgaggat gttgttccgg
     6601 aaggccatcg aagtaggcca cgtgttcaag ctggggacga agtattcggc cagcatgggg
     6661 gcgacctttc tggacgagaa cggcaaggcc cgcgagttca tcatgggctg ttacgggatc
     6721 ggcgtgaacc ggatcatggc cgcggcgatc gaggctcacc atgacgccga cggcatttgc
     6781 tggcccatga gcatcgctcc gttcgaggtg ctgatcgtcg ctctcgacac ccgagaagaa
     6841 ggcgtgatgt ccgcggctcg cagaattcac gaccgacttg cggaagaagg tatggacgtc
     6901 ctgctggacg accgggacgc acggccgggg ttcaagttca aagacgcgga cctgatcggg
     6961 atccccattc gcgtcaccgt gggcaagaag ggcctgacgg agggcgtggt ggaagtgaag
     7021 atgcgggaca gcaaggagat ggtgaagctg acaccggagg cggctgttgc cgaggtaatg
     7081 aactgggtta aggaggctaa agtactctaa tagctcttat ttggtgtcca gctttcagat
     7141 catggcgggc acctggactc gttctgctcg caaggtaaga cctcgtttgt tttcgaacat
     7201 ctcgactacg ccgtccaccg ccaggatcgg gttggcggcc gtcagatggc cgaaacgacg
     7261 gtaagtgttc gggaacaagc acgtttccac gaatccggtc cggtcggcga gggtcacgaa
     7321 cttcatcaga tcacccgtat tggtgcggtt aacgcggtcg gcgaccatca agccgcaaac
     7381 cgagacgcga cggccggtat gctcgttcaa ttccgagacc gggacatatc gcgaccagtc
     7441 gattgttctg cccttgtcgt cgcggctcaa atacgtgatg ggatcgattg tgatcgggaa
     7501 gccgagcaag tccatctcgc gtctggccac ctgccaggca tcgggacagt ccagatccac
     7561 cggcggggtc tggtcatcct tctcgggttg cgtcttccac agcgtgccgc cgtgttcgtg
     7621 ccccattcct tgcagccgtc tcaatcgcca gaacaacatc ggtcgcgaaa tgccgaacac
     7681 gtccagcgcc ccgctgtcca gcaacagccg cccctccgac tccgttggtc ggcagcgccg
     7741 cacgaaatcg tccacgcttt cgaagggccc cttttcgcgt tcatcgagat atcgttcgag
     7801 gaatgcctgc gacaattcct ggatgtgagt gacggggacg cggatcatta accgaaatga
     7861 atggtccgcg ggcgcggacc ctacagggtc ggcaccacgg ggataaaccc ctcttgtcgg
     7921 gccggcagga ttatccgtac ccttccggtg aatcccatct tgtttttcac tcgcacgatt
     7981 tgagaagata gcccaggtca tggcacctcg tggattatcc tgccggtctt cgggaccgtg
     8041 ccccgttgga agaaaaggaa gaccacgggg ataaacccct cttatcgagc cggcgggatt
     8101 atccggccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tcttcgggac
     8221 cgtaccccgt tggaagaaaa ggaagaccac ggggataaac ccctcttgtc gagccggcag
     8281 gattatccgg ccggtcttcg ggaccgtacc ccgttggaag aaaaggaaga ccacggggat
     8341 aaaccccgtg gctcgtcaag gcggggcggc tttcattcga attcggtccc tcaccgccgg
     8401 cgcgatgggg tacctggacg tgttccacgc tgaaacgctt gcgtgagcga ttgacgcatg
     8461 gggggaggaa accgacgccg aggcggaggg cctccatgac gtagaggatc tgggggaggg
     8521 tgggcgaatg ggcatagaag ccgcggtagt tcgacaggac cgccgccagg taatgggccg
     8581 gccaacggcg tttgagccag gcgccttgga aagcctcgac cgcgtactcg gccgagtggg
     8641 ccttgttgaa catgaagccg cggaagccct cgatcatggc ccagacttcg ttgatctccg
     8701 cctcgcttcg gccttgcaac cgggcggctt cgcgaaactc gccttccatt cggcggatca
     8761 tcgggagatt ttccttgttg agcgcccggc gcaggacgtc agcccggccg aggttcatgc
     8821 cggcgaactc gacggccgcc tggaggatgt gctcctcaaa gatcatcagg ccgtaggtgc
     8881 ctgccaggac ggaagttagg gctggatgca cctgtccggg cggttccagc tgctgatagc
     8941 gacgacagaa tgcctctttt ttaccttgat tggctgctcc gggacggatg atggccacga
     9001 tggccgtcag gcagtcgatg tcgcggcagt tggactggac cagcaggctg gtcatggccg
     9061 gggactcgat gtgatgcacg ccgcgggcat cgccgcgagc gatcatgtcc catgtttggc
     9121 cttccgagta atcgacatcc tgttcgaggt cgaccgtaat tccttccgtg cgatgcacct
     9181 catccaccgt atcgcgcagg accgacaggc cggcctggcc gagcagatcg atcttgatga
     9241 agcccagttc ctcgatcgcg tccatgtcgt attgggccac cagtgggcct ttcggcgacc
     9301 gctgcacggg cacgaggtcg gtcagcggtt cgggcgaaac gaccaggccg cacgggtgca
     9361 tcgaccagtg gcgcggcagg ccctcgaaga gtccggccac ccgcaggatc gagggaagcg
     9421 gttcttcctc cagcgacagc cggcgggctt ccggagaggc cggaatggcc ctggccaagt
     9481 cgtccgccga ggtgtgcggc acatatcgtg tgatttcgtg tacctcgtga ggcggcaggc
     9541 caagcacttt gcccagttcc gccaccaccg cgcggccgtg gaacctgttc ggcgaaccga
     9601 tcatcgccac acggtcgtag ccccatcgct cgtacaccca tcgcaccacc tcatcccggc
     9661 ggtcccaggg cagatcgagg tcgatatccg ccattttgct gaatctggcc cgctcgggat
     9721 tgaggaagcg gtcgaacggc aggtcgaatc ggaacgggca ggcatggctg acacccagcg
     9781 agtagcagac cagcgaatcg gccgcggagc cgcgagccag tactgcgatt ccccagcttc
     9841 ggcagtattc gacgatctcg tgaaagacca ggaagtattc ggcgtagtgc acctgctcga
     9901 tgacggtcag ctcgcggtcg agccgctcga gggcttccgc gagcgtcggg cgttttccgc
     9961 cgattccctt ctgctgcggc tgttcgacgt aacgacgacg acagccggcg acgcacaaat
    10021 cgcggagatg ttgggccgcg gaccggcggt cggaagactc gaaaggcggg aagcgattct
    10081 gcgacagatc gatggtcata ttgcatcgct cagccacctt catcgtgttg gccagggcgt
    10141 cggggcggcg atggaagagg tgtttcattt cggacggcgt ccgcagatgc cacgtacctt
    10201 gtggtttgtc ggggtgcggt tcgtcaagga gcgtcagtgt gccgattgac gccagtgccc
    10261 gcagggccgg catgtgccag cgtttcagac atcgcgactc acagcttgca agtacgggaa
    10321 tacccagttc atcagcgata cgtgcccgtc ggcgggcgac cagaacgtca tccggtccgt
    10381 gtaccgccaa ttccacatag agttgcccct caaacgcatc ccgcaggatc ggggtatacc
    10441 acggggataa accccgtggc tcgttccact gtcccagaag gcagatcagg ccctcggcat
    10501 gttcgcatag atcctgcaaa tcgaggggtg tttcacgtag atgatgcagc gagatcagac
    10561 gacagaggtt gccgtagccg gtcgggttct cggccagcag tgtgacggtg attctctcgg
    10621 cggtatggtc cgcgggcgcg gaccttacat gctgaatgtt gaatgttgaa tggtgaatgg
    10681 tgaatggtga acggtgaacg atcgtatggt ccgcgggcgc ggaccttact tggttttcag
    10741 agccacgggg ataaacccct cttgacgagg cggcaggttc atgctgccgg ttttcgcgat
    10801 cattcaccgt cggaagggaa ggaaggccac ggggatcaac cccgtggctc gtcaaggtca
    10861 gttccgctcc gaagatcggt ttgacgcctg cgcgctgaca ggctttctgg aactcgacgg
    10921 cggcatagag gccgttgaga tccgtaagcg cgatggcagg gatgccatgc tcggctgcaa
    10981 aagccgccaa ctcctccggt cgtgtcagtg acgagccgaa ggaataggat gagcggttgt
    11041 ggagggggat gggcatagcg atccgccatt cattccaaac cggcaagaca acctgctgta
    11101 agcatgtcct tgctgactgc tgaatgctga tcgctgaccg cttcccaatc atgtttcctc
    11161 atcatcgctt ttgttccgaa tcgttcgcga atcgcctggc aggcgatggc caggttgcgt
    11221 tgtttctgtt tcgcgccgaa caggtccatc tggctgaacc cgctgtagat gttcgagagc
    11281 tttacctgga ccatgcggat gcggacccgg cgatcccaca gccggtcaag caactttgac
    11341 agcaacgggt agacctgcat ttccacatcc gtgggctcgg ggagactcat ctggccctgg
    11401 tgctcgtcca tgtcggtgta gcggattttg acggtgaccg tgcgggcttg tttaccgtcg
    11461 gcccgaaggc ggagaaaagc ctcgtccgcc agccgacaca gggcggccaa gacctggttt
    11521 gggttcgttt tgtcttccgg gaatgtctcc tggtggccgt acgaaaaagc gtcagggcgg
    11581 gttagctcga ctgggcgagg gtcgatattg cgggcgaaat cgcgaatctg atgggctgcc
    11641 gaaccgacca attcggtcag ccagtccaat ggcatttcgg ctacttggcc gatccggagg
    11701 atccctgcgg actcaaacaa tctagctcca acctcgccga ttccgggcaa ccatttgacc
    11761 gcaagtggtt gcaggaacga tagttccgtc gactcgtcag gcgggatgac ggtcaggccg
    11821 ttgggtttgt ttagtttact ggctatctgg ctgatcagtt tggtccgggc cacgccacgg
    11881 gaaatcgtca ccttcagcca gtcgcggacg tcgcgtccga atttatccgc cacctctgcc
    11941 ggctcgcgtt tgcggcaggc aggggtaccg gtcaaatcca gatagccctc atcaatgccc
    12001 tgcttttcga cgatgggggt gatctcctcg gccatgccga agatgttttc cgagaattgc
    12061 tcgtatagtt cgaaccgcac tggcaggacg atgagtttcg ggcacagccg tctggcctgg
    12121 ctcatgggca tgggggtgcg gacgccgtat tgccgggctt catagctggc ggaggcgacg
    12181 attccgcggt ggagcccccc taccgccacg gggcggccac ggaggcggtt gtcggccgct
    12241 tgttcaacgg aggcgaagaa ggcgtcggcg tcgaaatgga gaaggaacat gagtgttcag
    12301 ttatcagttt tcagttgtca gggctcagtt tttcgctttg accttctgaa tcaacgatgt
    12361 gagcatccgt ttgacttcgg ccaagtcggt ggccaactgt ttatatctat cgatagcaag
    12421 catgccgagg tctcgcgcga gaagcagatg atattccagc tcacaagccg agcccatcgc
    12481 aatgtgcatg aaatgactca attccgagtt tgtttgcctt ccacaacctt cggcgatgtt
    12541 ggcacaggtg gatatggcgc ttcgccgcat ctggttcgtc agggcaaata tctcctcttt
    12601 cggaaagcgc ctcgtatcgc ggtatacggc caacaccaac tggtgccctt tttgccacac
    12661 ctgcagtttt cgaaaatcct gcatgatttc tcctttgtct tcgcactgac aactgaaaac
    12721 tgacgactga tcactgcact cttcgcacca ccgtatgcac caccccctga atcacgagtt
    12781 cgccgagggg catgaaggcc gggcatgttg ggttctcaga gatcaggtac ggacggccac
    12841 gttcgaccat caggcgtttg agcgtggact cgccgtcgat cagggcgacc acaatcgcgc
    12901 cgggtcgggc gacgggggtg atctcgccga cgacgatgtc tccgtcgtag ataccggcgt
    12961 tgaccatcga ctggcctcgg acgcgcaggg cgaagctgcg aggcgtggcc cgcaggatcg
    13021 ccgcggggtc aaggcgcgcg cacccttcgg ccaactcccc cggccagccc gcagcgatgc
    13081 ttaccaggtc gctctcatca atcccgccgt tgttgaccaa ggcgtttccg gcctggccgc
    13141 cagcagcttc attatgatcg taaagttttg tgcataagtc agttatgtca ataaatgttc
    13201 gcatggttag tcccccaata ctgacgatat attataacct aacaaaatcc gaactgcaaa
    13261 tagcaagaac atcgcaaagg ccccctggcg agggtacaat cctgcgaagg atggaagggc
    13321 gctatgcctg gtgagcagct tggcttcggc tgggaagaga cacctgcagc cccggtgccg
    13381 ccagcgcggg ccacgatgct gtcccagcgt ctcgcccgac tcgcccggcg gggtatctat
    13441 ctgggcacat cgagctggaa ataccccggc tggcttggcc agatctacac gcccgcccgg
    13501 tatgagacgc gcggccggtt ttcggagaaa aggttcaacg agacgtgcct ggaggagtat
    13561 tcgcgggtgt ttccgaccgt ctgtggagac ttcgcgttct accagtttcc gtcagagaag
    13621 acgtggcacg aaatcttcgc ccaagttccg aggggctttc ggttcagcct caaggttccg
    13681 gaggacatca ccgttgagcg ttttcccgat ctgcggcgtt acggccagag ggccgggcag
    13741 cggaatcccc acttcatgga tgcgtcggtg gtcaaatcgg agcttctcga tcgcctcgag
    13801 ccgtatcgag acaagctggg aatactgatc tttgaattcg gcaccattcg cgaggggcca
    13861 atggtcgatc cgaaggggtt cgcggccgca ctggaccgca tgttgtcgcg tttgcctctg
    13921 gaccattttc ggttcagcgt cgaagtgcgg aaccccgagt atcttgaatt cggtgcggat
    13981 tacctttcgt gtctcagagg acacggggta gcgcattgtt acaacagttg gactcgcatg
    14041 ccctcgctgc cccggcagat tgagctgccc ggcacattga ccgcgccgtt cgccacggcg
    14101 cggctgctgt tgcggcccgg gcgtacttat cagcaggcgg tcgatcgctt tgccccatac
    14161 gagaaggtgc aagacccata cccggagggc cggtcggcgt tggcgcggtt aatggagaca
    14221 tgtgttgcag agaatcgtga gctttttgcc tttgtgaata accggtttga aggtaacgcg
    14281 gtcgagacga ttgagggagt cattgagcgt tttgagtgac gcgatgtccg gcgggcgcca
    14341 agacggggtt ttacgtttta ttccttgaat ctgggcatga tgatgacccc gagcgtgcca
    14401 tagaccagac cctgggccag gagcaggctg cacggcagga ggatctcccg cagactgaag
    14461 ccccgccaga tgccgccctc gaaggcgacg atggcccact tgacggggct gaggtttccg
    14521 gccgtctcca tccaggacgg catgaacatc cgggggacca ttccgccgcc gagcatggcc
    14581 atgacgagca ggcatgccca tccggcgccg ccgacggacg attcggtgcg acccagcacc
    14641 gagagcagca ttgtcaggcc cacgaagcag agggcaacac aagggacgcc gagcagtaaa
    14701 gcgagcgggt tgtcgacccg gacaccgcaa gccacccggc cgaccagcag cagcattgcg
    14761 atgacgccga cgctcgcgac caggcaggcc aagccgttgc ccagcagaat ctgccaacgc
    14821 cggatggggg cgattcgcaa gcgaagccaa gtgcccgtct cgcgctcctt ggcaaaggcc
    14881 atggcgaact cggccgtgag gcccagcaag ccccagatca tgccaagcgg gaagcacacg
    14941 tcgaagggag accggggtgt gttgcgccag gcggtaaagg ggatgagttc gacattggac
    15001 agcgccggcc gggggccggc ggattccggc atcgaggtga ggcggggatc gaaggttgcc
    15061 aacagcgctt cgactgccgc tcggtcaagt gagcgtgacg gcccggaacg gccttgtgcc
    15121 ttcaaccatg cgtcgatcat ttcggatctt cggagcggat cgttccaccg ggcacggatt
    15181 actccggccg cggcttcgcg caacgcggcc tgcagataag ccagttcggc ggttctggaa
    15241 ggatcggcgc caaccgcgat cgggagcctc gcatcaagca gaccggcggg agtcaatttg
    15301 aaacctgcgg gcaacagaac aaacgcggag agcgagcctc gccgcacgca ggtttcagca
    15361 tcgtctcttg acgaccgact gatctgcagg atttcggact gcgacaaggc ggcgatgaac
    15421 tcccgcgagt cgctgttccg tgcctcgtca atcaccgcaa ccagcacagg ctgggactgg
    15481 ccgctgagga cgcccgcgaa catggtgccg atcaggatgg cgatcaatag tggaaagccg
    15541 atcatccacc agaagaaagc gaggcggtcg cgccaacaga ggagcagatt cttgcccgcg
    15601 attgccagga cggtgcgcat cagtcccgca acctccggcc ggtgagtctc aggaagaccg
    15661 tttcgagact tgggcgctcg atgctgaggc tccggatgcg aatgcccgac gcggcccacc
    15721 gtctgaccgt ttcaaacggg tgatccgttt cggccgacag ggacgtgccg ttgagacggc
    15781 cgggcaagga ccagtcgccg ggcggcgcct cgtcgagttc ggcgtgaacc accgcttgtc
    15841 cgccatggca atcgatcatg gccgatacgg tatccatggc caggatccgg ccgtgatcca
    15901 tgatcgcgac acgattgcag agtttctggg cctcctcgat gtagtgggtg gtgaagagaa
    15961 ttgtgcggcc ctgtcggccg agctgctcga tgtcctcgag gatatggagc cttgcctggg
    16021 gatcgacgcc gagagtgggc tcatcgagca ggagcacttg cggctgatgc aggagggcgc
    16081 acgccaggtg gaggcgtcgc ttcatgccgc ccgaataggt tttgaccagg tgacgggagc
    16141 ggtcggcgag gccggcctgt tcgatagctt gggcgacgcg ttcggacagt cgcccgtcgc
    16201 gcaggccgta gaggcgaccg aagaacttga ggttctccgt agcggtcata tcctcgtaga
    16261 gggagagggt ctggggcgcc aagccgatgc tgcggcgaac ccgcggccgt gtgggatcgg
    16321 ttgcgccgtc gatgtgaata tcccctgcgt cgggtttgag gagcccgacg agcatgtgaa
    16381 gcgtcgttgt cttacctgcg ccattggggc ccagaaggcc aaacgtctcg ccgcgccgga
    16441 cctcgaatga gatgccgtcg acggccctcg ttgagccgaa tgtttttcgc aggtggtcca
    16501 cgcggatcat ggcagcagta tacgtggcgg atgacgtttg tgaatcgttg cggacgagat
    16561 ctgtgatgat gattttgggc aagagtcttg gggtgaatcg gcgctgcgct cgttgctgcg
    16621 ccctacggtc aggctcatgg cgaggaggag accgagtcaa gccgggctac cagcgggatt
    16681 ggggcgaggc ggtctgtgta tcgggctgtt tgaccgggca tgcatgagct tttacactat
    16741 cgggctcgat atgatgcaac gtccgatgga ggtcaggtcg tgtggacttt catgtgccga
    16801 cgggccggac gtgccttcgc ggccgtcggg ggtatggggg tactcatggc ggcaaaccta
    16861 ggctgcttcc ctgaaaaatc gttcaagatg gacccggagg catcggttcg cgtttcggag
    16921 cgtctggtca acggggagga tggagcgtcc acgcgagtct ggctgaggcg gacgcaaagg
    16981 gagcttgccg ggttgttgcc tgactcggtt gcgagcccac tactcactga agaactggtg
    17041 aacggcgacg gacgggctgt ggacgtcctg cgtcacttcg gcgtcagccg gcagagtctt
    17101 cagagcattg tcaagaacta tcggggaatc cgctattcgg ctcaagcggc cagccgggac
    17161 tactgcattg agaagcaggc agcggcctgg ccgggcttcg aggacgtctg gattcccatg
    17221 cgtctggcgg ttgacgacca gctcgagctg tcgggccgtc tgggctatgc ccgcaacggg
    17281 aacggcgaga tcatcgaagc ggactgcgtg gtgatcctgc cggggctatt cggcgatcac
    17341 ggcgtcaagc gcagccgcga cctggcgatt ccgctgcgtg agtccgggtt tcatgtgctg
    17401 cacctggagc ttcgcggcca cggtcagacg gagtcgcggt acccgcggat gtatcacacg
    17461 ttcggcgtca tggagaccga cgacctgatg caggtgtcgg actaccttga gcagttgccg
    17521 cacattcgcc ggacggggct gatcgcctat tgctggaacg ccagtattgc cctgctggcg
    17581 gcgtggtacg atggccgatt gcctgacgat cctcagattt cgccggcaat caggccttac
    17641 ttgcagtgcc cgccggaacc tttgcgtcgc cgcttctcgg ccggcattat cgcattttcg
    17701 ccggtggttc gttgggaagt gctgatggat gagctggatc ggcagcgatc gatctgggcg
    17761 catccgatct atgcggcgat ccaggacacg atccgggacc gcatggtgcg caaggggtat
    17821 cctgaaagga gcgggagtct gcgcaacctg attgagtatg agcataacgg ctacggtatt
    17881 cccatgccgc acggggcgcg ggaggggtat ccgtttcttc ggctgatttc gtacaacgga
    17941 gaaccatcgg gggacaaact ggagttcgcc cgcatgccgg tgctgatcgt tcatggagcc
    18001 gacgacccgc tggtgccggc tcaggacgtt gccgacctga tggcgacggt agacaatccg
    18061 agggtggggg cgatcatttt gccaagcggc gggcacgtgg ggtttgcggc atattgtccg
    18121 aggtattatt tgagcttgat caccaacttc ttcgatccgg tcaacggtcc ggccggtcgc
    18181 ggtgcggcca taagcgccgc ggcccggtga cggcccaagg ctgcggaatc aaaggggcag
    18241 cgatttccaa ggcggagcgc cttcttgcgg gccgcaagcc aagcgtaccg cgatccggaa
    18301 acacgcgagc aagccgcaca gatgtccggc cgtagggttg taacgacccg caaggctgaa
    18361 tctgctccca acgcaaaaga agaacatgtg ctgcgggctt taagtggcat ggtgctcaaa
    18421 taggcaagat tcacaaacct acatggtagt acgtcccgag ggccaattcc tacgcatacg
    18481 tatgctcatc gtttatcggc agatcgcaca acgacggatg gagaacaggc gcaggcactg
    18541 tttttgcaag tagttacgac atccgcgggt tggttcacga cttcgtggca cacgggttgc
    18601 agatgaggaa cggcgtgttc cgacctcaaa acgggccgga tgatcaacaa cgccgaggaa
    18661 caggactatg aatgcgaagc tgacaacgaa gcggtcgccc gagaacgact gttcgacaca
    18721 cgcaaacgtc agccgaggca aatctgatca atcgcctctt tccggcccca agttgaaaga
    18781 gagccttgaa cgcctcgaac ggcaactggt catcgaggca ctgatacgaa ccaaagggaa
    18841 tcaatcacgc gccgcccggt ctctgggcat taccgaacgg atcatggggt tgagggtgaa
    18901 gaagtatggt atcgatccac agagcattcg cgacgcttac cagtctgacc cgattgtgca
    18961 gcaatgctca tcggccgctg cgctacgaac aatgagcaac gaagagaagg atcatgtccg
    19021 ttcttcccgc tgatgtaacc gggttacaat cccgattgcc ggaggagact actcgtgaat
    19081 gagaatacat tcaaacgttt gatgggaaaa ctactaccac tcctggcagt cctggcaaca
    19141 gccatactgg ctactcgcgc tttcggtcag gagactcagc cgccgtcggc cagcgacatc
    19201 ctcgacaagg tttcattgct gtcggtcgac gtggataccc tgtgggttct gattacggca
    19261 tttctggtat tctggatgca ggccggattt gcgttggttg agaccggtct gacccgggcc
    19321 aagaacaccg tgaacatctg catgaagaac ctgctggact tctgttttgc cagcatcgga
    19381 ttctgggcga tcggctttgc gatcatgttc ggaacgggca acaagtggtt cgggacaagc
    19441 ggctggtttc tccaaggtga tgcggggacg acgtttgcgg ccctgtcatg gacttccgtc
    19501 agcctggatg ccaagttcat tttccagctc gtctttgcgg ggacggccgc aacgatcgtg
    19561 tcgggcgcca tggccgaacg cacgaggttc accagctaca tgctgtacag cctggcaatc
    19621 accgtattca tatacccggt cgccggtcat tggatctggg gcggagggct tttatcggcc
    19681 ggcaacatgt tcggcgaagg caaaggcatg tttgacttcg ccggctcgac cgtggttcac
    19741 tcggtaggcg gctggctggc gctaaccggt gcgattgttc tggggccgcg aatgggcaaa
    19801 tacggcccgg gcgggaaggt caatgccatt ccgggtcaca actttccgct ggcgatcctt
    19861 ggcgtgttca tcctctggct gggctggttc ggcttcaacc ccggcagcac catgatggcc
    19921 accgggtgcg gagcagccat cgctcacatc gcagtgacca cgaacttcgc agcggcgacg
    19981 ggaacgatcg gcgccctgct gacatcgaag ctctggttta gcaagtggga cgccagcatg
    20041 gcgggcaacg gctgcctggc cggcctggtg gcggtgacgg caccatgtgc attcgttgac
    20101 acctgggcgg ccggactcat cggcctcgtc gcgggcgttc tggtagtcgg ctccgtcgtc
    20161 ttcatcgacc gactgctgaa agtggacgac ccggtgggtg cggtttccgt ccacctggtt
    20221 aacggtatct ggggcacttt ggccctcggc ctgttctcca acccggtaca cggttcgacc
    20281 gggccgctgc cggggctgtt ctatggcggc ggtgtcgagc agctcaaaat gcagttgatt
    20341 ggcgcagtcc tggtcgggat ctggtgcctg ctggccggtt tcgccctgtt cctgggattg
    20401 aaggctctga cggggttgcg tgtcagcccc gaagaggaac tcagaggcct cgatgtcgac
    20461 gagcacggca tggaaggtta tccgaacttc caacttctga agcactgagt atcctggaat
    20521 cgttcccggc atcgcgggcc ggttcctgaa ccgagtgagg gattgtgtga taggacgaca
    20581 agacttccga actcctgaca ccgaccgtcg agagacggat cattcctccg ctgaaaggag
    20641 gcttggatga agaaaatcga agccattatc aagccgttca agctggacga ggtcaaaacc
    20701 gctctgaccg cgatcggtat ccaagggctt acggtgagcg aagtccgcgg gttcgggcgt
    20761 cagaaaggcc acaccgagca ttaccgcggg gccgagtaca ccgtggattt catacccaag
    20821 gtgaagatcg aagtcgtcgt gcccgaagcg aaggtggccg aggtcctgga gaccatcgcc
    20881 aaagccgccc gcacggggca gatcggcgac ggaaagatat tcgtttcatc gctcgacgag
    20941 atcgtgcgga ttcgcaccgg tgaaaaagga ccggcggcga tttgacctcg ttcttcctga
    21001 ccatgggaac aagcaactca gcgctgcggc gcttgcgtcg ggcggaaaaa gctctggccg
    21061 acgcgatcga gcacttcctt gttctccgca cgggacgcgg cgtcgctccc ggagccgaag
    21121 gcggcgatcc tcaactccgg ctcccatctt tcggcggctg accggagcgg ttgagactct
    21181 tcgattctcc agacaggttt ccgggaacat gcggtttgca caggtgctca atgagcaacc
    21241 accccttacc gacgaccgat tccgaatcgg atccctcacg gctgaaagag catttcatcc
    21301 tgggacggga gcggatcaga catatgtatc acagcggtgg ctcagccgct gaggtcagta
    21361 aggccctcac ggccctgtgt gactcggtgg tcaggcaagc gtacctggcg gcgatccagg
    21421 atcttccgcc ggatgaccgt gtttccgtgc tcggctcgct ctcgctcgtt gcggtcggcg
    21481 gatacggtcg cggcgacctt gcgccgtatt cagacctgga tctgctcttt ctcaccgtct
    21541 ctcagccgtc ggcggtcacg agggatttcg tgtctcgact tgttcgggat ctctgggatg
    21601 cggggcttca tctgtcgcaa agcgttcgaa gcccgggcga gtgcggctcc ttcgcagaga
    21661 ccgacttcgc cgcaaggacc gcgctgttcg aaacccgcct gattgtcggt gatcccgagc
    21721 tctacacgaa cctgcggcta cgcgtctgtc ctccgcgagg ccaccgggcc cttcgcgcgt
    21781 tcatcaacca ggctgtcgcc tctcgatccg aagaacacga ggactacttc agccggacgg
    21841 cgctgctgct cgaaccgaat gttaagaaga gtcccggcgg cctgcgggat gtccatctcc
    21901 tgcgctggct ggcaatggcg cgttacggcg actcagacct gaaccgtttg cgggccgacg
    21961 gcatactagc ggccgaagag gccgacacga tcgtcggagc gcatgagttc ctccgcggaa
    22021 tccgtcacgc ccttcatttc cacgccggtt ccgcccagga tgtgctgact cgcgaggaac
    22081 aactgcggct ggccgaacaa tacggctacc gacagcaagg tgccctcctt cctgttgagt
    22141 gcttcatgca acagtactac cggcacacgg ccggccttcg cgacctgctg ttgcggttca
    22201 tcgaacggct tgaaagacgg tcaccggtcc gcaggctgta cagccgcatc ctgagtcgcc
    22261 gcgtgacccc cagcatcgtt gcctaccccg accatattga gattgtcggg ggcaccgatg
    22321 gccgttcctt cggtcaggcc gacaccgtgc ttcaggcgtt cgatctggcc agacgattcg
    22381 gcgtgccggt gcactccgac acgctatccg aaatacgaca acacctgccg gcctgcatcg
    22441 tcaccactgc cgaacgccgc aggtttctgg aatgcctcgc ccagccgcac ggcctgggca
    22501 ctctgctccg gaaccttcac ggcgtcggcc tcctgcaacg gctcatcccc cctttcgagc
    22561 acgcccgcgg cttgatccag ttcagtctgc tgcaccacta cacgattgac gaacacgtga
    22621 ttcgagctgt cgaggccgct gcgcgtcttg cggatgaacg cactccgctg ggcctggcgt
    22681 atcgtgatat ccggcgcaag gaccttcttc accttgccct gctgctccac gacctcggga
    22741 aaggtcttgg ggaagaccac tgtgaagtcg gccgacagat ggccgaggac gctgcccgag
    22801 acttcgattt ggatgaacat gaagcacgac tgctcgtgtt tctcgttcgt cgccacctgc
    22861 tgatggccca cacttcacag cggcgcgacc cagccgacgc cgataccctc ctccagttca
    22921 gtcgactggt cgcaacgccg gagttgcttc gcatgctcta tgttctaacc gccgccgaca
    22981 ccgaggcggt tgcgcccggt gagttcacca cctggaagga atccttgctc accaacctct
    23041 acgcccgcgc gatggaatgc cttaccggcg aaacgccgtt gggtgatgct gcaaacagat
    23101 tggcggccgt gcgttcctcg cttcacgaaa gactggccgg cacctttgac cccacttggc
    23161 tcacaggaca actcgacgcc atgcccccgt cctacctgac cggcacggag ccgcggcgag
    23221 tgaaagcgca cttgcggttg ctgcacggta tggatggggc tggtgtccga gtcacggccg
    23281 aatatcgtaa ggatctccag ctttcagagt acaccgtgct cacccacgag agactgatcc
    23341 cgggcatctt ctcgaaagcc gccggagctc tcgccgcatc gggctttcag atcgtcgacg
    23401 cgcagatcat cacccgaccg gacggcgtcg ttctggacac gttccgcggc ttggacctgg
    23461 atttttcagg cccgccgccg cccgaacgga tgacagaaat cggagaacgt atagagcgcg
    23521 tgctcctcgg gcaggaaacg gtcgaggcgc tgataacccg gcgaggcgaa ccgttgagcc
    23581 gaggacctca tgccggtccg caggaacccg ctcaggttga gatcgacaat tccagttccg
    23641 atcgcttcac aatcattgag atcttcgcca atgaccgtct gggcctgctc tacgcgatca
    23701 ccaggacgat gtttgagatt gatctttctg tgcactccgc gaaaatctca acccattaca
    23761 gccaggtggt tgacgccttc tacgtcacca ccaacaacgg aagcaagatt gacgatccgg
    23821 ccttgatccg gcgcattcgg cagcgtctgc tcgaagtggt gtgagaagtc taaggcggac
    23881 cgcctttttg cggaccgcaa cccaagcgtt ctgcgatcca gaaa
//
LOCUS       DAOTPW010000063        23718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2213_length_23718_cov_5.552424, whole genome shotgun sequence.
ACCESSION   DAOTPW010000063 DAOTPW010000000
VERSION     DAOTPW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23718
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_23718_cov_5.552424"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1454)
                     /locus_tag="P5316_07670"
     CDS             complement(84..1454)
                     /locus_tag="P5316_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR84873.1"
                     /translation="MTADDVRLSHPRERAPDDKPKRGILLRLIRGLAMYLLPVVVIVA
                     GIAGAVHLIRTAPQAQRRTPENPAALVRVEMVRRVSANAVVQAQGTVMPSQEVLLQPR
                     VSGEVTELSPKLVPGGRFKTGEFILQIDRQDYKLAVEKTKSQVAQAEYELKLEQGHQE
                     IARREWELLDMKEQAGALDFELALRKPHLLKATAALEAAKAALREAELNLDRTTIRAP
                     FNCVVRSENVDLGAQVAPQTQLAILVGTDQYWVRAAVPVDHLRWVLFPDEHGEGGSKA
                     AIRQELGTGPQGEWCGMVVRLLSDLEPQGRMARVLISVPDPLNLNEMNKRSHPLLIDA
                     YVSVDIEGREVNDVFALPRTALRQGRQVWIMNGRNELEIRDVDIVWGNRDTLLVRSGM
                     QEGERLVVSDLPAPVAGMALALEEPPPRPNAADGRDFRERADRPWSRQCRICTIALNN
                     AARSPG"
     gene            complement(1451..3037)
                     /locus_tag="P5316_07675"
     CDS             complement(1451..3037)
                     /locus_tag="P5316_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR84874.1"
                     /translation="MRILQVMPWCLCPAICCGACTAFLPQRTAPPEGPIPTRFTLGNT
                     DVAESNRWWQDFESEELNRLIDVALRENLSLRQLWARLEQAGCQVVMAASGLYPQVTV
                     GADAAYRRTVTKVDSEASSLKSRLGETVANEVSKGISKALGQDSGATSSSGSAPGTQE
                     QSSPSRLIRETKSFGLSLAANYEVDVWGRVASAYRAARFDFAASREDLESTAMTLVAE
                     VAERWLRILEQQELQRLLSEQLATNKIYLELVELRFRKGQVSALDVYQQRQAVSEVER
                     LLPLSEAEEQVLRHDLALLMGKPPTADLTVGLYDLASVPDPPGTGIPAELLARRPDVR
                     GALSRLHAADHRVASARADRLPAILLNGGIGYDTGDIAHLFDDWFLNLAAALTAPLFD
                     GFRRQAEVDRTLAVVEQRLSEYRLAVLTAIKEVEDALIRERKQCEHIQGLSRQLEDAR
                     NALREASGRYQAGLSDYLPVLAALERTQSLARTLVVARRELLVTRIDLYRALGGTWTQ
                     ELEPPVRASDEAAEPKDSES"
     gene            complement(3571..5913)
                     /locus_tag="P5316_07680"
     CDS             complement(3571..5913)
                     /locus_tag="P5316_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRR84875.1"
                     /translation="MKRLLKTSTVLWCLLATGLAVVRSPRASVADTLEENKPIGTAAQ
                     QQQWIEGLGLVGLGRIDKAAEIINSLATTGVSDERVAHVRDWLSEYGDFEKSRRERVG
                     KDYHKYVSWAKEDVAAGRRHQALVSISLAHSVADNPDDFLKEAWVVEALENAVKAAQE
                     YEAKGKWYAAARVYVRLQDLYPLNRDYRKALERCQDHIRLELTYEPESDWESAVADIS
                     KDMAYDAFLKISTDYLKTPDFREAVLGALRQVLLMTKTPKLPKVFEKLNDKEAVSDFS
                     TRVEARLRFAEAKDSLTVHELIAHFERVLEINEENGLFPQPVLVREFTYGALQPLDKF
                     SDMIWPADVPEFNKHTQGRFSGVGIQIRKNDGEPILVVSPLKGSPAYRAGVRPGDLIT
                     RINGKNAAKYTITRAVREITGPVGTTVNLTVKRPGQEQEFELTLERQEINVESIKGFD
                     RDDHDAWKYMLDENQKIACVRLTNFTENSADELKKVLTDLTKQGLRGLIFDLRDNPGG
                     PLKAAVDVSGMFLPPNKPIVSTRDRNSSPWEVSSPGDVSYTDFPMIVLTSRYSASASE
                     IVTGALQVHKRALVLGERTYGKGSVQQVLPLNRSRMAFLKLTTQLYYLPDGRCLHKDD
                     DSVTWGVDPDISARVFPKERFKLYELQLKRDILKGKDQGELTDDDIQRVTSYRNLAAS
                     QPGKGESGKGKDDTGDETPTASSKPATQAETGEGEETEDEDDAAEDFVTREDPNDYPE
                     SDPQLDLALLLMRIRLETNEPWPSRATQMAATPAPANAQP"
     gene            6673..8052
                     /locus_tag="P5316_07685"
     CDS             6673..8052
                     /locus_tag="P5316_07685"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRR84876.1"
                     /translation="MAKKAVSGSIRTYRSALTFLDSMVNHEKMTRVGYTPRNFNLARM
                     TRLLSGLGNPHREFKSVHIAGTKGKGSTAAMLSAMLSNCNLKVGLYTSPHLLDVRERI
                     QIDGKMISEQDMTHLMGRIAPVVRRLGKDEPTYFEILTALAFLYFAEQQLDIAVVETG
                     MGGRLDSTNVLRPEVCAITSISYDHMGQLGLTLDKIAEEKAGIFKPGIPAISAPQAPE
                     AKAALQRVAAKVKCPLLFTGKDIEFSYRFESTRPIGPHTCVSLITANSRFEHLPVPLM
                     GEHQAINCGLALSVMATLKERGFKLEDQSVIEGLAKAKLPGRMETIHENPRIMVDGAH
                     NAASVEALMRAIGQNIPYDSMVVIFGCRSDKDISGMLNHIQLGADKVIFTEGGIPRAA
                     DPGELAAEYIERCGKMAQVAHTLEEALEIAERAVTREDIICITGSFHLVGHAKRLIAS
                     RTAAGSVAS"
     gene            8262..9599
                     /locus_tag="P5316_07690"
     CDS             8262..9599
                     /locus_tag="P5316_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84877.1"
                     /translation="MAGLNPHRSIVSLTLLAVVICVQGGACLPRTTPDEGKGNRAGET
                     VRKTIGSEGGVIEAINSRGDTIRLEIPSGALSGSVDISVDPLDEPPANPIGENVFPGV
                     DLAPDGLMLLKAATLKVSPAEGTVDARALLFHLRQADFVVPISEQEVNGGVISGKIRH
                     FSSYLGGSPSGNEASQQAGQAAGQSQGSSHWQSTLDDVGGLLQWGEWFQSQGMESEAQ
                     DSFKEAEERLREAIECFLDPGCHVVPIDICDDDYVREALAYLGQAQLLGFDEESELMQ
                     DLHTAVTHLLNQCTNRFAIEYDYLQTVDYASYSHDIHVTGQVLFSLPIYVVGDIGALQ
                     ATGQGTVNGTITGAADECTITGSFTINVVVEGELEADEMGQPWLNLRLNESWYASGQQ
                     TVVCPDESSDLPLFPVQSVQNVRLLMQDGYVLEQPHLETQGHYRWTLHVLHLW"
     gene            complement(9713..10441)
                     /locus_tag="P5316_07695"
     CDS             complement(9713..10441)
                     /locus_tag="P5316_07695"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HRR84878.1"
                     /translation="MPDKRSHRGPHPDDARLFAESQWPILRAAVGDLSFLLGRGYAEK
                     SALKLVGDHFGLTQRQRIAVMRCSCSDDALATRRQREVPPGAVSASELAVDGYNVLTT
                     VEAALAGGVVIQGRDGCYRDMASMHGTFRKVRETGPALGLIGETVAALRPQRCLWLLD
                     SPVSNSGRLRSQMRDLAARRGWTWEIELVTNPDTVLADSSAIIATADSVVLDRCRSWL
                     NLARLVLTFHLPNARIINLAPENR"
     gene            11703..12767
                     /locus_tag="P5316_07700"
     CDS             11703..12767
                     /locus_tag="P5316_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84879.1"
                     /translation="MPAKARLCVACGFDTKTGRRMQDTAISGGGSGALTAVGGVLGSA
                     VRSSGALLLGTLLSFLGAMLGAAIWAGVAYAIHLQIGWIAWAIGGLAGGGMYLGYRNQ
                     STPAGLIASFMAVLGIVVGKIGVFVIVVLSVAASMMPAMSNSGAVHTAVRSEGEETAM
                     MTERYVLASHHATLKANRQGLSYDDPQREALYAQEQQRFKDMPEDRVKKAMEEMKQWE
                     QGGKWEDAAYVRDFLIYSYIDEALETEASKRGVDTDELEVNAAQWAQYHRAAAKRADS
                     IAENQRVVEAKRIERDRNEESERALVEAEKEFEDAAGKAAIGLLGLFFSALFGWMDVL
                     FIILAVATAYHLASGLSFGG"
     gene            12929..19090
                     /locus_tag="P5316_07705"
     CDS             12929..19090
                     /locus_tag="P5316_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR84880.1"
                     /translation="MSKSFPRHAIAFSTLLAVLILTASVNRADAGQVCTPIFDPALQP
                     PGTNAKITALCVFNDSLYAGGEFSLAGDVSAGCIARWDGRHWSALGTGMANDPGVTAT
                     PQVAVIAVYNNHIYAGGFFTRADGQPADHIARWTGSAWAPVGSGVNGPVVAMMVYNNE
                     LYVGGSFTQAGGNPASNIARWNGSAWTAVGSGANGHVLAMTVLDGALYAGGPFTEAGG
                     VTANRIARWNGSWSAVGSGFDGTVQALGVLGGQLYAGGTFSKSGAASVSKIARWDGTT
                     WSALGSGIVGSVACMATFDDRNGEALYVAGTFSQAGGLTANQIAKWDGTAWSTLGTGT
                     NNGVKALAVFSDDISPALFVGGLFTIANGKTANYIARWACPWDGDKDGIPDVEDNCPK
                     KSNADQADGDGDGVGDACDNCRTIANPDQTDTDGDGVGDACDNCPTVKNANQADNDQD
                     SAGCDGRPDCGGDACDNDDDNDGVLDVSDNCPKTANPANTEPTDCNGDGDTDDADEAV
                     GLQCDRDGDGIGDTCDNCPDVTNPDQKDTDKDGLGDVCDPDADNDGILNDGDGSGIAG
                     DNPCTGGNKHECDDNCPLHFNPDQEDPDGDGKGNPCDNDDDGDGRADSQDNCPLVYNP
                     SQTDTDKDGVGDACDNCPKVANVNQTNSDNDSLGNACDNCDFADNPPHTEPTDCNGDG
                     DTTDPDEAVGKQCDQDGDGVGDACDNCPTVPNPDQANNDGDEFGDACDDDDDNDGVLD
                     DDDNCPLHYNPDQTDSDGDALGDACDNCPEISNSDQADADGDGIGDACDNCPNKAGFD
                     QMDTDGDGLGDLCDNCAGIDNPDQTDADGDGIGDACDNCPNKAGADQTDSDGDGLGNL
                     CDNCAYVSNPDQVDTEGDGVGDACDNCPLVRNANQRDSDGDGTGNACDNCPFEPNAGQ
                     ENRDGDGAGDACDLCPDDPDKIAPGACGCGMADTDADSNGVPDCLDKDTCPEDPDKRA
                     PGQCGCGNPDTDTDGDGVADCVDSCLNDPGKVTPGVCGCGIPDRDSDNDGVLDCREEC
                     PHDPNKTTPGECGCGVPDVDSDGDGVLNCHENCPYDPAKTEPGGCGCGTPDIDTDSDG
                     VLDCNETCDNDPNKTSPGICGCGTPDTDTDGDGTADCVDACPVDPAKTAPGQCGCGNP
                     DTDTDRDGKADCVDQCPEDPLKIAPGTCGCGTPDTDTDQDGVADCHDLCPEDAGKINS
                     GICGCGQPDTDSDSDGEPDCVDPCPDDPDNLCGDDECPDDPAKHVPGICGCGVPDTDS
                     DDDGTPDCNDRCPDDPGKSEPGTCGCGVPDTDSDDDGSADCADPCPDDPLDQCGDLCP
                     DDPNKTSPGICGCGTPDTDSDGDLTPDCRDACPQDPDKVAEGICGCGVPDVNEDEDDF
                     MDCVDPCPADPANTCVVDLCPDDPKKIEPGVCGCGIPDDDTDQDGTLDCLDGCPADAG
                     KTEPGLCGCGVADLDQDGDGTPDCLDDCPIDPNKTQPGLCGCGVSDADTDSDGVPDCG
                     DNCPADAAKVEPGICGCGTPDTDSDRDGVPDCNDRCVNDPNKSQPGVCGCGTPDTDTD
                     GDSIPDCTDGCPTDAGKTEPGWCGCGTADTDQDGDDVPDCLDNCPFDPDKAQPGLCGC
                     GVSDADTDSDGVPDCSDNCPADAAKVEPGTCGCGTPDTDSDSDGVPDCNDGCMNDANK
                     SQPGACGCGIPDTDSDADGTPDCNDQCPGDADKVSPGACGCGVADTDTDGDLTPDCLD
                     PCPEDPLDQCGDLCPDDPNKTSPGICGCGVSDTDTDSDGTPDCNDGCPEDAVKLAPGL
                     CGCGTPDADRDQDGTADCHDLCPDDPNKIAPGVCGCGVSDADTNNNGIADCQESGGGG
                     GGGGGGGGGLPPIDNCPNDPLKTEPGVCGCGVPDTDSDGDTIPDCIDNCPFDVGLDQT
                     DTDSDGPGDLCDNCPLTANPSQLDSDGDGVGDLCDNCPLTPNSDQLDSDTDGVGDACD
                     LCPNVANPSQTDTDGDGVGDECDNCPETANPDQSDRDGDGPGDVCDPSPDGGSGQPIP
                     NDGQTPSSRRVWCGLGATQAAMVGALLLMLIRPRRRR"
     gene            19589..20488
                     /locus_tag="P5316_07710"
     CDS             19589..20488
                     /locus_tag="P5316_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84881.1"
                     /translation="MKTGKVLFSGRDIVCFDWDRQVFELTRERAMDLMSLPPMLQRDF
                     VVRDREGEIYRGCFMSALSSSSYDGPTIGSDFFEVEGIRPPLYHIAGGYPGEHGPGAK
                     LRFDPRLRQHLDTAGLLRSIKEGEKVEPVETVFCGWHGEALGRRAAIMFFPETIRMGL
                     DIRLVLRFHKTKAATGSPAERERDARRGITRDPAISQEPDRVEAHITLRSNDGKSERT
                     RKITVPVENLIEGYSSAFPLVVWPWSESGRSVDDLFKPGPAELTVSIVATRKTDKGIE
                     QIGIWDIPTRQVQILPGPHREGK"
     gene            20560..21267
                     /locus_tag="P5316_07715"
     CDS             20560..21267
                     /locus_tag="P5316_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR84882.1"
                     /translation="MSQRRILVVEDDAAIRQGIVDALQFDGYATLEAANGDVGLDMAR
                     AVDCDLLLLDLVLPGADGLTILREVRITRPTLPVIILTARGEEQDRVQGLKLGADDYV
                     VKPFRVAELLARVEAVLRRSPERPADLRLVAFAGGVIDLARSEIRFENNDRCELSERE
                     VELLRYLACNNGRAISRDEILSRVWRLDPIGIATRTIDMHIARLRDKLRDDPEDPKII
                     LTVRGKGYMFANCGGGG"
     gene            21267..23288
                     /locus_tag="P5316_07720"
     CDS             21267..23288
                     /locus_tag="P5316_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR84883.1"
                     /translation="MRPWLTWPAFVVCIAVILAAMGWHSQTVLQLDRADAAAIRRAAI
                     EERIRLALWRMDSALTPLVAQESTWPYYAYASFAPTERAYTNLLNPVQPGEILMASPL
                     LDPPSRHVLVHFQINPDNSISSPQVPTGPMRQIAEGQYTTADKIELYTRRLAALQPIL
                     SRANLLSMLEPAEPPPVRIAVLPTASQPSADQESLAQQSEVQSFWGLSNQLPDRDLRR
                     RLGRLDEQIYTQQAVTPQPAAQEQGQVSGLSQGGNPRQDRAQRPSSSGKQVRSSGKTS
                     WISPDQQDLMNTYDAQARGYNYDVNGELILTNAGNSRVMRGGVKEGSARSLWIGDTLV
                     LARRARVNGSEYIQGCRFDWPGIQSWLLGEIRDLLPEARLEPADLELDQTDTRRLAAL
                     PIRLVPGDIPSPPAQGMSPVRMTLLVAWSCLLLAAAAVIILLVGTMSLSERRAAFVSA
                     VTHEMRTPLTTFRMYTEMLTGGMVPSEEKRRRYLDTLRVEAERLSHLVENVLAYARLE
                     KNRAISAAQNVTLAELIARVEKRLAERAAQAGMKLEVSRPEDICSSTVHVDPSAVEQI
                     LFNLVDNACKYAVSTGDRRIRLETLRDGGKATVRVRDYGPGIPEREARKLFRPFCKSA
                     KDAANSSPGVGLGLALSRRLARHMGGDLRLDQSVNDGACFVLTLPATVN"
BASE COUNT         4729 a         7307 c         7387 g         4295 t
ORIGIN      
        1 ttcctgcttg attcgcagag ccatgaacag gccgcccagc agcatgaaga gcatcagcag
       61 attcggggcc acggagtgcc gggtcatcca ggcgatcgcg ccgcgttgtt caaggctatt
      121 gtgcagatcc ggcattgcct gctccatggg cggtccgcac gttctcggaa atcacggccg
      181 tccgcggcat tgggtcgggg ggggggctcc tccagcgcca aggccatccc ggccaccggg
      241 gcgggcaggt cgctgacgac caggcgctcg ccttcctgca tccccgatcg caccaacagg
      301 gtatcgcggt ttccccaaac gatgtccacg tcccgaatct cgagttcatt cctgccgttc
      361 atgatccaga cctgccggcc ttggcgcagg gcggttcttg gcagggcgaa aacatcattc
      421 acttcccgcc cttcgatgtc gacgctcacg taggcatcaa tcagcaacgg atgcgaacgc
      481 ttgttcatct cgttcaaatt caacgggtcg ggtactgata tcaagacccg tgccatacgg
      541 ccctgcggtt caaggtcgct cagcaatcgc acgaccattc cgcaccattc cccctgagga
      601 cccgtaccga gttcctgtcg gatcgcagct tttgaaccgc cctcgccatg ttcatcagga
      661 aaaagaaccc atctaaggtg atccacgggc acggccgccc gcacccaata ttggtccgtg
      721 ccgaccagga tcgcgagttg ggtctggggc gcgacttggg cgccaaggtc gacgttctcc
      781 gatctcacga cgcagttgaa aggggcccgg atcgtcgtcc ggtcgaggtt cagctctgcc
      841 tcgcgaaggg ccgcttttgc cgcttcgagc gccgcagtgg ctttgagcaa atgaggcttg
      901 cgcaaggcca gttcgaagtc cagagcgccg gcttgttctt tcatatccag cagttcccat
      961 tcgcgtctgg cgatctcctg atggccctgt tcgagcttca gttcatactc cgcctgggcc
     1021 acctggctct tcgtcttttc caccgcgagc ttgtagtcct gccggtcgat ctggagaatg
     1081 aactcgccgg tcttgaagcg tcctccgggt accaacttgg ggctcagttc ggtgacttca
     1141 ccgcttaccc tgggctggag caggacttcc tgggacggca tgaccgtgcc ctgggcctga
     1201 acaacggcgt tcgcgctgac ccgccggacc atttcaactc gaacaagtgc tgcggggttc
     1261 tccggcgtac ggcgttgggc ctgcggtgcc gtcctgatca ggtgaacggc acccgcgatg
     1321 cccgcgacga tcacgaccac cgggagaagg tacatggcga gaccgcggat cagtcgcagg
     1381 agaatccctc gtttgggctt atcatccggt gcccgttcgc gagggtgtga aagacgtacg
     1441 tcgtcggcag tcatgattct gaatccttcg gctcggcggc ctcgtcgctt gctcggacag
     1501 gcggctccag ctcctgtgtc catgttccgc ccaatgcgcg gtacagatcg attcgcgtaa
     1561 ccaagagctc gcgtcgggca acaaccagcg tccgggccag tgattgcgtc ctttccaggg
     1621 cggccagcac cggcaggtaa tcgctcaatc cggcctggta ccgccctgat gcctcgcgca
     1681 gggcgtttct cgcatcctcc aattgtcgag aaagtccctg gatgtgctcg cactgctttc
     1741 gctctctgat cagagcatct tccacttctt tgatcgcagt gaggaccgcc aaccgatact
     1801 cggacagacg ttgctcaacg acggccagcg tccgatccac ctcggcctga cggcgaaacc
     1861 cgtcaaataa aggcgccgtc aaggcggcgg caaggttcaa gaaccaatcg tcgaacaggt
     1921 gggcaatgtc ccccgtgtcg tagccgatgc cgccgttaag aagaatcgcc gggaggcggt
     1981 ccgcccgggc cgacgccaca cggtgatccg cggcatgcag tcgtgacaag gccccgcgaa
     2041 catcgggtcg acgggccaac agttcagccg ggatgcccgt ccccggcgga tccggcaccg
     2101 aggccagatc gtagagacct acggtcaagt cggccgtggg cggcttgccc atcaataggg
     2161 ccagatcgtg gcgaagcacc tgttcctcgg cttccgaaag ggggagcaaa cgttcaacct
     2221 cgctgaccgc ctgtcgctgc tggtacacat cgagggctga cacctggccc tttcgaaacc
     2281 gaagctcaac aagctccaga tagatcttgt tggtcgccag ttgctccgac agcaagcgtt
     2341 gtaattcctg ctgctccagg attcgcagcc atcgctcggc cacttcggcg accagtgtca
     2401 tcgcggtcga ctccaagtct tcgcgcgagg cggcaaagtc gaaccgagcg gcacgataag
     2461 ccgaagcaac acggccccac acgtccacct catagttggc ggccagcgac agcccgaaac
     2521 tcttggtttc cctgatcaat cgcgagggcg acgactgttc ctgcgtgccg ggggcgctgc
     2581 cggaagaaga agtcgctcct gagtcctggc cgagtgcctt gctgatacct ttcgagactt
     2641 cgttggccac agtctctcca agccgggact tcaacgacga cgcttcgctg tcgaccttcg
     2701 tgaccgtgcg tctgtaagcg gcatcggccc cgaccgtcac ctggggatac aatcccgagg
     2761 ccgccatgac cacttgacag cccgcttgtt cgaggcgggc ccagagttgc cggagcgaca
     2821 ggttctcccg cagggcgacg tcgatcaacc ggttcagttc ctcgctctca aagtcctgcc
     2881 accagcgatt gctctcggcg acatcggtat tcccgagggt gaatctcgtc gggatcgggc
     2941 cctcgggtgg ggctgttcgc tgcggaagaa aagccgtgca ggcgccgcag catattgcgg
     3001 gacacaggca ccacggcatc acctgcagaa tcctcatgcc gcatcctcat ccctggccgg
     3061 catactcccc aacgccgccg ggcaaggccg ggttgaatgg tcgacccgcc gttcgacgtt
     3121 ttcagcaaaa cctggaagct gaggaagcat gtgcatagct gcgcgtccaa gaagccggtg
     3181 tcatgacagc gtggaagccc gccacacaac gtttcaaaac gcacttgagg gacttggcgt
     3241 ccatgcaccc gatgatcata tcactccaca acgccgtcag catcctgtct tagccgcctt
     3301 agaagacccc catgattgat ggccgctctt tcaacagaag acccgatcag caactcaaac
     3361 cttggcccaa tgaaactgaa tgacacattt gtttcaaggc caagcagatg tctcttgccg
     3421 acactgtttg tgcggccatt gacgagcctg agaccgccat ttgccctcga tcgactgcct
     3481 acgagccggc tttccaggcg gacccgtcat ttcagcggcc ccgaagacga gaggcaaggg
     3541 gtttgtgcgc cggttgtgcg gcgagtgcga ttacggctgg gcgtttgcgg gagccggagt
     3601 cgcggccatc tgggtcgccc ggctcggcca gggttcgttt gtctccagcc ggatgcgcat
     3661 cagcagaagc gcgaggtcca gttgcggatc gctttccggg tagtcgtttg gatcctcgcg
     3721 ggtcacaaag tcctcggcag cgtcgtcttc atcctccgtt tcctcgcctt cgccggtttc
     3781 tgcctgggta gccggttttg agcttgcggt cggagtctca tcgccggtat cgtctttgcc
     3841 cttcccggat tcacccttgc ccggttggct ggcggcgagg ttgcggtagg acgtcaccct
     3901 ctggatgtcg tcgtcggtca gctcgccctg atccttgccc ttgagaatat ctctcttgag
     3961 ctggagctcg tagagtttga atcgctcctt ggggaacacc cgcgcggaga tgtccgggtc
     4021 gactccccac gtgaccgagt catcatcttt gtgaaggcag cggccgtcgg gcaggtagta
     4081 aagctgcgtg gtcagtttga gaaaagccat acggcttcga ttcaacggca agacctgttg
     4141 cacactgccc ttgccatagg ttcgctctcc caagaccaag gcccgcttgt gaacctgcaa
     4201 agcaccggtc acgatctccg aggcgctggc actgtagcgg ctggtgagga caatcatagg
     4261 gaaatcggtg taagagacgt cgccgggaga tgagacctcc cagggggagc tgtttctatc
     4321 ccttgtggag acgatgggct tgttgggcgg caggaacatg cctgagacgt ccaccgccgc
     4381 cttgagcggg ccgccaggat tgtcccgcag gtcgaaaatg aggccccgca ggccctgctt
     4441 ggtcaggtcg gtgaggactt ttttcaactc gtcggcgcta ttctccgtga agttggtcag
     4501 gcggacgcag gcaatcttct ggttttcatc gagcatgtac ttccatgcgt cgtgatcatc
     4561 ccgatcaaac cctttgatcg actcaacgtt gatctcctga cgttcgagcg tgagttcgaa
     4621 ctcctgctcc tgcccgggcc gtttgaccgt caggttcacg gtggtgccca ccggaccggt
     4681 gatctcccga accgccctgg tgatcgtgta tttggcggca ttcttgccat tgatcctggt
     4741 gatcagatca ccggggcgaa cgccggcccg gtatgcgggc gaacctttga gcggcgagac
     4801 gaccaggatg ggttcgccat cgttcttgcg aatctggatg cccacgccgc tgaagcggcc
     4861 ttgcgtgtgt ttgttgaatt cgggcacatc ggccggccag atcatgtcgc tgaacttgtc
     4921 cagcggttgc agtgctccat aggtgaactc gcgaacgagc acgggttgcg ggaacaaacc
     4981 gttctcttca ttgatttcca gaacccgctc gaaatgcgct atcagctcat gcacggtgag
     5041 cgaatctttg gcttccgcaa acctgagtct ggcctcgacg cgggtggaga aatcagacac
     5101 cgcctccttg tcgttgagtt tctcgaagac cttgggcagc ttcggcgtct tggtcatgag
     5161 cagcacttgg cgcagggctc ccagcacggc ctcgcggaaa tcgggcgtct tgaggtagtc
     5221 ggtggagatc ttgagaaagg catcgtatgc catgtcctta gagatgtctg cgacagccga
     5281 ctcccaatcc gactccggtt cgtaagtcag ttccaggcga atatggtcct ggcaccgctc
     5341 cagagccttt cggtaatccc tgttgagggg atagagatcc tgcaaccgga cgtagacgcg
     5401 ggcggccgca taccacttgc ccttggcctc gtactcctgg gcggccttga cggcgttttc
     5461 aagggcttcg acgacccaag cctccttgag gaagtcgtcc gggttgtccg caacgctgtg
     5521 agccaacgaa atgctcacca gcgcctggtg acggcgtccg gcggcgacgt cctccttggc
     5581 ccaagaaacg tacttgtggt agtccttgcc gactcgctcc cggcgacttt tctcaaaatc
     5641 accgtattcg ctcagccaat cgcgaacatg ggcgactcgt tcgtcgctta ccccggttgt
     5701 cgcgaggctg ttgatgatct ccgcagcctt gtcgatcctg cccaagccaa cgaggccgag
     5761 tccctcgatc cactgctgtt gctgagctgc ggtaccgata ggcttgtttt cttcgagcgt
     5821 gtccgccacg gatgctcgcg gcgagcgcac caccgccaga cctgtggcaa gcagacacca
     5881 caacaccgtc gacgtcttga gcaggcgctt catcagcact ccttcagatt cacattcacc
     5941 ctggttgcgg tcatcgcgcc agggcaggtt ggccggccta caggaccgag ctgccgcccg
     6001 tgccatcggc cggattggcg gaaccgagat tcgccaggac ggattgtcgc gcggctcgtt
     6061 ggggtggcac tagctctccc cggacaggcg tcgtcaaccc caaaagctta attatatata
     6121 cgacctagac atcggcaatg ccgcacctgg cgtcagacag gtgcaaaacc tctggcgagt
     6181 ctcggcgccg aaggtccctt ttcgcatcac gtcggttcgt gatttcggct ttctctgact
     6241 gcaaattcgc ggcagttctc ggcaaggcgt ctaccgtcac tcgcgcggct tgtcggcagg
     6301 caggtgtcgg atttggacgg cggatgcgaa acaaggagag ccgcactctg taaccgattg
     6361 tcaaaaagca agttagacga cagccattgg cggctgatcc tcgggtctgg gtgaggtttg
     6421 acaagcgaac tgaaggccag cctgggacga ggccccgacc tgcccggaat agtgtcgatg
     6481 cccagcgttg gccgacccca ggccgacagc aaacccaaga agatgcaata agtctctaag
     6541 ctcaatccaa ataaatagat acgagaattg atgccctgtg attttgtgct cggtatatag
     6601 cgagggattt gactgggatc gggctttttg atagtatggc ggcctatggc tcggtccacg
     6661 acggcaaaga caatggcaaa gaaagcagtt tccggctcca tccgaaccta tcggtcagca
     6721 ttaaccttcc tggactcgat ggtaaaccac gagaagatga cccgggtggg gtacacacca
     6781 cggaacttca acctcgcgag gatgacccgg ctgttatcgg gcttgggcaa tcctcatcgg
     6841 gagttcaagt cggttcacat tgccggaacc aagggcaagg gctcgaccgc agcgatgctg
     6901 tcggcgatgc tttccaattg caatctcaaa gtggggctgt atacatcgcc gcacttgctg
     6961 gatgttcgag agcgcatcca gattgacggc aagatgatca gcgagcagga catgacccac
     7021 ctcatgggcc ggatcgcacc ggtggttcgc cgtctgggta aggacgaacc cacctatttt
     7081 gaaatcctga ccgctctggc gtttctctat tttgctgagc agcagctcga catagcggtg
     7141 gttgagaccg gcatgggtgg gcggttggac tccactaacg tgctgagacc ggaagtctgc
     7201 gctatcacga gcatcagtta cgatcacatg ggtcagcttg ggctcacgct ggacaagata
     7261 gccgaggaga aggccggcat tttcaagcca ggaattcccg cgatcagcgc cccccaagcg
     7321 cctgaggcca aggcggctct tcagcgcgtt gcagccaagg tgaaatgtcc gctgctgttc
     7381 accggcaagg acattgagtt cagctaccgc ttcgaatcga cacggccgat tgggccgcat
     7441 acgtgcgtca gcctcatcac ggcaaacagc cgtttcgagc atctgccggt acccttgatg
     7501 ggagagcatc aggcgatcaa ctgcgggctg gcgctcagtg tcatggcgac gctcaaggag
     7561 cgaggcttta agctggagga tcagtcggtc atcgaagggc tcgccaaggc gaagcttccg
     7621 gggcgcatgg aaaccatcca cgaaaaccct cgaatcatgg tcgatggagc ccacaacgca
     7681 gccagcgtcg aggcactgat gcgggcgatc ggtcagaaca tcccatacga ctcgatggtc
     7741 gtcattttcg gctgccggtc tgataaagac atcagcggca tgctgaacca tatccagctt
     7801 ggagccgaca aggtgatctt caccgaagga ggcattccgc gagccgcaga cccgggggag
     7861 cttgccgccg agtacatcga gcgatgcggc aagatggccc aggtggccca tacccttgag
     7921 gaagccctgg agatcgcgga acgagcggtg actcgcgagg atatcatctg catcaccggt
     7981 tcgttccacc tggtcggcca cgcgaagagg ctgattgcct cgcgaaccgc cgccggttcc
     8041 gttgcctcct gaaaaaaagc gccggcggta gtgacggttc acggcgctgc ggtgtgccct
     8101 gctcttggga tggtcgatcg tccggccaat gcgacccaga gtgtagctgt cagtcggcca
     8161 gaccatatcg cggtcgagag aagacattga tcgacaccgg tgccggtgat agagtacgaa
     8221 gcgtctgtct cggcgttcat ctcaagacaa ggagaccgca catggccggc ttgaatccgc
     8281 accggagcat cgtgtcgctg accctgctcg cggtagtgat ctgtgttcaa ggcggagctt
     8341 gcctgccccg aacaacgcct gacgaaggta agggcaaccg cgccggtgag acggtgcgca
     8401 aaaccatcgg atctgagggc ggcgtgatcg aggccatcaa cagccgcggt gacacgatcc
     8461 gcctggagat accgtcgggc gcgttgtcgg gatctgttga catctcggtc gacccactgg
     8521 atgagccgcc ggccaacccg atcggggaga acgtgtttcc tggcgttgat ctggcaccgg
     8581 acggtctgat gctgctgaaa gccgccacat tgaaggtctc gccggccgag ggaacggttg
     8641 acgcgcgggc tctcctgttc catctgcgac aggcggactt cgtcgtgccg atttcggagc
     8701 aggaggtgaa cggaggcgtg atttccggga agatccggca cttcagcagt tacctgggcg
     8761 gatcgccgtc cggtaacgag gcctcgcaac aggccggcca agccgccggt caatcgcaag
     8821 gctcatccca ctggcaatcg acgcttgatg acgtcggcgg cttgttgcag tggggcgaat
     8881 ggttccagtc acaaggcatg gagagcgagg ctcaggattc gttcaaagaa gccgaggaac
     8941 gcttgcgtga ggccatcgag tgtttcctgg atccgggttg tcatgtcgta ccgatcgaca
     9001 tctgcgacga cgattacgtg cgcgaagccc tggcttacct cggccaggct cagttgttgg
     9061 gcttcgacga ggaatcggag ttgatgcaag atcttcacac tgccgtgacg cacctgctca
     9121 accagtgcac gaaccggttc gccatcgagt acgactattt gcagacggtc gactacgcct
     9181 cgtacagcca cgacatccac gtcaccggcc aggtcctttt ctcgctgccg atctacgtcg
     9241 tcggtgacat cggggcgctg caggcaaccg gccaaggcac tgtgaacggc acgatcacgg
     9301 gggctgcgga cgagtgcacc atcaccggca gttttacgat caacgtcgtg gtggagggcg
     9361 aactggaggc cgacgagatg ggtcaaccct ggctgaacct gcgcctcaat gagagttggt
     9421 atgccagcgg acaacaaacc gtggtttgcc cggacgagag cagcgattta ccactgtttc
     9481 ctgttcagtc cgtccagaac gtccgtctgc tcatgcagga cggctacgtt ctcgagcagc
     9541 ctcatttgga gactcaggga cactaccggt ggacgctgca cgtgctccat ctgtggtaag
     9601 cggcttccga gagcttctgc gaagacctgt gggcggcgtc ttcggctcgt ggagatgagg
     9661 accaagcccg cgcagccgcc aggatggcaa cgaaggtgat ctgccccgag cgtcagcggt
     9721 tttccggagc aaggttgatg atgcgagcgt tcggtaagtg aaaggtcagc accaagcgag
     9781 cgaggttgag ccaggaacgg catcggtcaa ggacgacgct atcggcagtt gcgatgatgg
     9841 cgctggaatc ggccagcacg gtatcagggt tcgtgacgag ttcgatttcc caggtccacc
     9901 cccgcctggc cgcgagatcc ctcatctggc tccgcagtcg accggagttg gagacggggc
     9961 tgtcgagcag ccagaggcag cgttggggcc ggagggcggc tacggtctcg ccgatcaggc
    10021 ccagtgccgg gccggtttct cggaccttgc ggaaggtccc gtgcatgctg gccatgtcgc
    10081 ggtagcagcc gtcccgtccc tgaatgacga ccccgccggc cagagcggct tcgacagttg
    10141 tcaggacgtt gtatccatca acagccaatt cgctcgcgct cactgccccg ggcgggactt
    10201 cgcgttgcct ccgtgtagcc aatgcgtcat cggagcagga gcacctcatc accgcgattc
    10261 tctgccgctg cgtcagtccg aagtgatcgc caaccagttt gagggccgat ttctcggcat
    10321 aacccctgcc aagcagaaaa gacaggtctc cgacggcagc tcgaagaatc ggccattggg
    10381 actcggcaaa cagtcgggca tcatccgggt gagggccgcg atgcgatctc ttgtcaggca
    10441 tagggtgttc ctgtcaaccg gtgccggatc ggctcaatta cccaaggcgg accgcctttt
    10501 tgcagaccgc aatccaagcg cactgcgatc cagaaacatg cgcgcaagcc gcacagacat
    10561 ccagccataa ggttctaagg cggaccgcct ttttgcggac cgcaacccaa gcgctctgcg
    10621 atcaagaaac atgcgcgcaa gtcgcacaga tgtctggccg taaggttcta attctacctc
    10681 tcccaaccca aatgacatac cgccgaggac cgtttccata agcggccaga tccgtgacgc
    10741 tgtagccctt ctttcacaat cccgagcgat cgataccgcg aaggacgggt cagacgcaag
    10801 gcgaacacgg ttccgcgagg cattcggcgt gtacatacct tgggggccac gggcggttcg
    10861 tccgaccgag caggcccggc gggcagaggg taccggcaaa caagcggcca gcggcagctc
    10921 cagaattctc tcgaacccat tgaattacca ccttggtagg aagggttatt tcggttagca
    10981 tatgccatga agtgcttgcg gaagcctctc gacgtgcggc cgcgtcgcgg catgggccag
    11041 caatctgggc catatcggtc ttgggcgcgt cttgcaccca atagcggcag aggaagactt
    11101 ccgcggtttt tcacttcccg gaggtcgttg cactatggag gacacgcaat cagagcgtat
    11161 caccgtgagg tgcgagtgtg gcgcaaagct gggactgcgg gcctcttttg ccgggcacaa
    11221 ggccaagtgt cccaagtgca gcagatcctt catcatacca gcaactgacg gtcgcgcaag
    11281 catcgcagca cccgcgtatc caagcccggg gatgcccaag caggagtccc gacccgcaac
    11341 ggcgagccag aagaccgtca tggtgggttg tccggcgtgt ggcaagcaca tccgcgtgcc
    11401 cgcgaacaca accggccgaa aggcgcgctg tccggcctgc gagggtgtgt tcgtcatcgg
    11461 tcatgcagcc gcgagtccca cggttgggat tcaaccggga ccggtatcgg aacctgcggc
    11521 gaaaccggcg ccgaagcccg ctccggtttc ggagtcgcct ctgccagagg aataccaaaa
    11581 gcaggattga tggtacttac ggatggtcgg acgatggtgg ttccctgctg accgaggcgt
    11641 cgcagcagcc ggccgcagac atcggcacgg catacaagct gtgcccgaac tgtggccaga
    11701 ccatgcctgc gaaggcccga ctgtgtgtgg cgtgcggctt tgacaccaag accggacgtc
    11761 gcatgcagga tacggccatc agcgggggcg gaagcggcgc actgaccgcc gtcggcggag
    11821 tactcgggtc ggccgtcagg tcgagcggcg cgctgttgct gggcacgctg ttgagcttcc
    11881 ttggagcgat gctgggcgcg gcaatctggg ccggcgttgc ctatgccatt catcttcaaa
    11941 tcggctggat cgcatgggcc atcggcgggc tggcgggagg cggcatgtac ctcggttacc
    12001 gcaaccaaag cacgccggcc ggactgatcg cctcgttcat ggcggttcta ggcatcgtcg
    12061 tcggtaagat cggtgtcttc gttatcgtgg tcttgtcggt ggcggccagc atgatgcccg
    12121 cgatgagcaa ctctggcgcg gttcataccg ccgttcgcag cgagggtgag gaaaccgcca
    12181 tgatgacgga gaggtacgtt ctggcctccc accacgccac tttgaaggcc aatcgccagg
    12241 gattgagtta tgacgatccg cagcgggaag ccctttacgc ccaggaacag cagcgattca
    12301 aggacatgcc tgaggatcgg gtcaagaagg ctatggagga gatgaagcag tgggaacagg
    12361 gcggcaaatg ggaagatgcg gcctatgttc gagactttct catctattcg tatatagacg
    12421 aggccctgga gaccgaggcg agcaagaggg gcgttgacac ggacgagctc gaagtgaacg
    12481 ctgcacaatg ggcacaatat caccgggcgg ccgccaagcg ggccgacagc atcgcagaga
    12541 atcagcgagt ggtcgaggcg aagcgaatcg agcgcgatcg gaacgaagag agtgagaggg
    12601 ccctcgtcga agccgaaaaa gagttcgagg acgccgccgg caaagctgcc ataggactgc
    12661 tcgggctgtt tttcagcgca ttgttcggct ggatggatgt cctcttcatc attctggcgg
    12721 ttgccacggc ataccatctg gcgtccggtc tgtcgttcgg cggttgattg caacgcaccc
    12781 ccgttttcag tactgtccgg ccggctcatt ccggccgata gggctgtttt ctgctcaacc
    12841 cgtcgggtcc aagctcacct gatgccgata aacctgatag gcagaatgga atctgccacg
    12901 gatagtccac gcgccaaagg atgggcccat gagcaagtcg tttccccgtc acgcaatcgc
    12961 attctccaca cttctggcag ttctgatcct gacggcttcc gtgaaccggg ccgatgcggg
    13021 tcaggtctgc acgccgatct tcgacccggc tcttcagccg ccggggacaa acgccaagat
    13081 caccgcgttg tgcgtcttca acgactcact ttacgcgggc ggcgagttca gcctggccgg
    13141 tgacgtttcg gccggctgta ttgcacgatg ggacggccgg cactggtcgg ccctgggcac
    13201 cggaatggcc aatgatccag gcgtgaccgc tactccgcag gtggcggtga ttgccgtgta
    13261 caacaaccac atctacgccg gtggcttttt taccagggcc gacggtcagc cggccgacca
    13321 catcgcccgc tggacgggct cggcttgggc acccgtggga tcgggcgtca atgggccggt
    13381 cgtggccatg atggtgtaca acaacgagtt gtacgtcggc ggctcgttca ctcaggccgg
    13441 aggcaacccg gcctcgaaca tcgctcgctg gaacggctcg gcatggaccg cggtgggcag
    13501 tggagccaac ggccacgttc tggcaatgac tgtgcttgac ggggcccttt acgccggagg
    13561 cccattcact gaggccggcg gcgtcaccgc taaccgcatt gcacgatgga acggctcgtg
    13621 gtccgccgtc ggcagcggat ttgacggaac ggtgcaggct ttgggcgtac ttgggggaca
    13681 gctttacgcc ggtggaacgt ttagcaaatc gggggcagcc tccgtcagca agatcgcccg
    13741 ttgggacggg acaacgtggt cggcgctggg cagcgggatc gtcggtagtg ttgcctgcat
    13801 ggccaccttt gacgaccgta acggcgaggc tctctatgtc gccggcacgt tcagccaagc
    13861 gggcggcctg accgccaacc agatcgccaa gtgggatggc actgcctggt caacgttggg
    13921 cacgggtacc aacaacggcg tcaaggccct ggcggtgttc agcgacgaca tcagcccggc
    13981 cttgttcgtc ggcggcctgt ttaccatcgc caatggcaag acggcgaact acattgcccg
    14041 ctgggcatgc ccatgggacg gcgacaagga cgggatcccc gacgtagaag ataactgtcc
    14101 caagaagagc aatgcggacc aggcggacgg cgatggcgac ggcgtcggcg atgcctgcga
    14161 caactgccga accatcgcca acccggacca gacggacacc gatggcgacg gcgtcggcga
    14221 cgcttgcgac aactgtccaa ccgtcaagaa tgccaaccag gccgacaacg accaggactc
    14281 ggcgggctgc gacggccggc ccgattgcgg cggcgacgcc tgtgacaacg acgacgacaa
    14341 cgacggcgtc ctcgacgtca gcgacaactg ccccaagacc gcgaacccgg ccaacaccga
    14401 gccgaccgac tgcaacggcg acggggatac cgacgacgcc gacgaagcgg tgggcttaca
    14461 gtgcgaccgc gatggtgacg gtatcggcga cacgtgtgac aactgtcccg acgtcaccaa
    14521 ccccgaccag aaggacaccg acaaagatgg tctgggcgac gtatgcgatc cggacgccga
    14581 caacgacggg attcttaatg acggcgacgg cagcggcata gccggcgaca acccttgcac
    14641 cggcggcaac aagcatgaat gcgacgacaa ctgcccgctg catttcaatc ccgaccagga
    14701 agaccccgac ggcgacggga aaggtaaccc ttgcgacaac gacgatgacg gcgacggacg
    14761 tgcggacagc caggataact gcccgctggt ttataacccg tctcagaccg acaccgacaa
    14821 ggacggcgtc ggggatgcat gcgacaactg ccccaaggtg gcaaacgtca atcagaccaa
    14881 cagcgataac gattccctcg gcaacgcttg tgacaactgc gatttcgcgg acaatccccc
    14941 acacacggaa ccgaccgact gcaacggtga cggcgatacg accgaccccg atgaggccgt
    15001 cggcaaacag tgcgaccagg acggcgacgg cgtcggcgac gcctgcgaca actgcccgac
    15061 cgtgcccaac cccgatcagg ccaacaacga cggcgatgaa ttcggcgacg catgcgatga
    15121 tgatgacgac aatgacggcg tgctggacga cgacgacaac tgtcctctgc actacaaccc
    15181 cgatcagacc gacagcgacg gcgacgcctt gggcgacgcc tgcgacaact gccccgagat
    15241 ttccaactcg gaccaggctg atgcggacgg cgacggcatc ggcgatgctt gcgacaactg
    15301 cccgaacaag gccggcttcg atcagatgga taccgacggc gacggtttgg gcgatctgtg
    15361 cgacaactgc gccggaatcg acaaccccga ccagacggat gccgacgggg acggcatcgg
    15421 cgatgcttgt gacaactgcc cgaacaaggc cggcgccgac caaacggact ccgatggaga
    15481 cggcctcggc aacctgtgcg acaactgcgc ctatgtgagc aaccccgatc aggtggacac
    15541 cgaaggcgac ggcgtgggcg acgcctgcga caactgtcca cttgtcagaa atgccaatca
    15601 gcgggacagc gacggcgacg gcaccggcaa cgcctgtgac aactgtccgt ttgagccgaa
    15661 cgccggccag gagaaccgcg acggcgacgg ggccggcgac gcgtgcgacc tgtgcccgga
    15721 cgatcccgac aaaatcgcac cgggagcctg cggatgcggc atggccgata ccgatgccga
    15781 cagcaacggc gttccagact gcctcgacaa agacacttgc cctgaggacc ccgataagcg
    15841 agcccccggc cagtgcggct gcggcaaccc cgacaccgac accgatggcg acggagtcgc
    15901 cgattgcgtc gattcgtgtc tcaatgatcc aggcaaggtc acgcctgggg tttgcggttg
    15961 cggtattcct gatcgcgaca gcgacaacga cggggtgctc gactgcagag aagaatgccc
    16021 gcacgatccg aacaagacca cccccggcga atgcggatgc ggagtgcccg atgtggactc
    16081 cgacggcgac ggcgtgctga actgccatga gaactgtcca tacgacccgg ccaaaaccga
    16141 gcctggcggg tgcggctgcg gcacaccgga cattgatacc gacagcgacg gcgtgctcga
    16201 ctgcaatgag acctgcgaca acgatcccaa caagacctca ccaggaatct gcggctgcgg
    16261 aacgccggat acggacaccg acggggacgg cacggccgac tgcgtggacg cctgtccggt
    16321 tgaccccgcc aagaccgcgc ccggtcagtg tggctgcggc aaccccgaca ctgacacgga
    16381 ccgcgacggc aaagccgatt gcgtggatca gtgccccgag gacccgctca agatcgcacc
    16441 cggcacctgc ggctgcggga cgccggacac ggataccgat caggacggcg tggccgactg
    16501 ccacgacctt tgtccagaag acgccgggaa gatcaactcg ggcatctgcg gttgcggcca
    16561 accggatacg gacagcgaca gcgacgggga acccgactgc gtggatccct gccctgatga
    16621 tccggacaac ctctgcggag acgatgagtg cccggatgac cccgccaaac acgtgccggg
    16681 catttgcggc tgcggggtgc ccgataccga cagcgacgac gacggcaccc ccgactgcaa
    16741 cgaccgatgt cccgacgatc cgggcaagag cgagcctggc acctgcggct gcggggtgcc
    16801 tgacaccgat agcgacgacg acggaagtgc ggattgtgcc gacccctgcc ccgacgatcc
    16861 gctcgatcag tgcggcgacc tctgccccga cgatccaaac aagacgtcgc cgggaatctg
    16921 cggatgcggc acaccggaca ccgacagcga tggagacctc acgcccgatt gccgcgatgc
    16981 ctgccctcag gatccggaca aagtggcgga aggaatctgc ggatgcggtg tgcccgacgt
    17041 gaacgaggat gaggacgact tcatggactg cgtcgacccc tgtcccgccg atccggcaaa
    17101 cacgtgcgtc gtggatctgt gtcccgacga cccgaagaag attgagccgg gagtctgcgg
    17161 ttgcggcatc cctgacgacg acaccgatca ggatggaacc cttgattgcc tcgacggctg
    17221 cccagccgac gccggcaaga cggaacccgg cttgtgcgga tgcggagttg cggacctcga
    17281 tcaggacgga gacggcacac ccgattgcct ggacgactgc ccgatcgatc cgaacaagac
    17341 gcagccgggg ctttgcggct gcggcgtctc tgatgctgat accgattccg atggcgtacc
    17401 tgattgcggt gacaactgcc cggccgatgc cgccaaggtc gaacccggca tctgcggatg
    17461 cgggacccca gataccgaca gtgatcgcga cggcgtaccc gactgcaatg acagatgcgt
    17521 gaatgatccg aacaagtcgc agcccggcgt gtgcggatgc ggaaccccgg ataccgacac
    17581 cgatggcgac agcatacccg attgtaccga tggttgcccg accgacgccg gcaaaacgga
    17641 acctggctgg tgcggatgtg gtacggctga tacagaccaa gacggtgatg atgtgccgga
    17701 ttgcctggat aactgcccgt tcgacccaga caaagcacag ccggggcttt gcggctgcgg
    17761 cgtctctgat gctgataccg attccgatgg cgtacctgat tgcagcgaca actgcccggc
    17821 cgatgccgcc aaggtcgaac ccggcacctg cggatgcgga acccccgata ccgacagtga
    17881 cagcgacgga gtgcccgact gcaacgacgg atgcatgaac gatgcgaaca agtcgcaacc
    17941 cggcgcgtgt ggctgcggga tcccggatac ggattcagac gcggacggca cgccggactg
    18001 caacgatcaa tgcccgggag acgccgacaa ggtttcgccg ggagcttgcg gatgcggcgt
    18061 ggccgacact gatacggatg gcgatctgac ccctgactgc ctggaccctt gccctgaaga
    18121 tccgctcgat cagtgtggcg acctctgccc cgacgatccg aacaaaacct cgccggggat
    18181 ctgcggatgc ggcgtgtcgg acacggacac cgatagcgac ggcaccccgg actgcaacga
    18241 cggctgcccc gaagatgccg tcaagcttgc cccaggcctc tgcggatgcg gcacgcccga
    18301 tgccgatcgg gatcaagacg ggacagccga ctgccacgat ctgtgccccg acgacccaaa
    18361 caagatcgct ccgggcgtct gcggctgcgg cgtttcggac gcggacacca acaacaatgg
    18421 aatagctgac tgccaggagt ccggcggtgg cggaggaggg ggcggcgggg gaggaggagg
    18481 cttgccgccg atcgacaact gcccgaacga cccgctcaag accgagcctg gcgtttgcgg
    18541 atgcggtgta ccggacaccg actcggatgg cgataccatt cctgactgca tcgacaactg
    18601 cccgttcgat gttggcctcg atcagacgga cacggactcc gatggtccgg gcgatctgtg
    18661 cgacaactgc ccgctgacgg ccaatccgag tcaactggat agcgacggcg acggtgtcgg
    18721 tgacctgtgc gacaactgcc cgctcacccc caattcggat cagcttgaca gcgataccga
    18781 cggtgtaggc gacgcctgtg acctgtgccc gaacgtcgcg aacccgtctc agacggacac
    18841 cgatggcgac ggcgtgggcg atgagtgtga caactgcccc gagacggcga atccggatca
    18901 gtcggaccgg gacggtgatg gaccaggcga cgtttgcgat ccctcacccg acggtggctc
    18961 gggccagccc atccccaatg atggtcagac cccttcatcc aggcgggtat ggtgcgggct
    19021 gggtgcgacc caggcggcga tggtcggcgc actgctgctg atgctgatcc gcccccgtcg
    19081 gcggagataa gaccggtccc acgcgccaca tccctttgcg acacccgggc gaaccgccaa
    19141 ggtgttacac acccgttaca ccccggcgtc caaacacagc gtagatttct atgtagccga
    19201 cgctccaccc tctaccaagg agtctgccat gacaagccgt caccatcctc gcctgattgt
    19261 cgccactgtt ctgtgtgccg ggtgccgggc gcgatgctca tggctctgcg cgggcaggcc
    19321 gattccaacg ctccggagga ctgtttcgtt gaacgcaagt atctttcatg gcatcctaag
    19381 gcggaccgcc tttttgcggg ccgcaaccca agcgctctgc gatccagaaa catgcgcgca
    19441 agccgcacag atgtctggcc gtaaggttcc aatgacgttc ttgttgccgg tcgcaacact
    19501 cgccgcagcc ctgtggcagc cagcagggac cacaacgcct gtgacttccg aaccagccgc
    19561 tgaccgagcg gccgtggagt gggtcgacgt gaaaaccggc aaggtcctgt tctccggccg
    19621 ggatatcgtt tgcttcgact gggaccggca ggtctttgaa ctgacccgtg aaagagcgat
    19681 ggatctcatg tccctcccgc ccatgcttca acgcgacttt gtggtccgcg accgggaggg
    19741 cgagatctac cgcggctgct tcatgagtgc gttatcatcg tccagctatg acggcccgac
    19801 cattggcagc gacttcttcg aggttgaggg tatcagaccg ccgttgtatc acatcgccgg
    19861 aggctacccc ggtgagcacg gtccgggtgc caaactacgg ttcgatcccc gcttgagaca
    19921 acatcttgat accgccggtt tgctgcgcag tattaaagag ggcgagaagg ttgaacctgt
    19981 cgaaaccgtt ttctgcggct ggcacggcga ggccttgggg cgccgagcgg caatcatgtt
    20041 cttccctgag actatacgca tggggcttga cattcgactc gtgctgcggt tccacaagac
    20101 caaagcggca acaggctcgc cggccgagcg cgagcgagat gcacgtcgtg gcatcacgcg
    20161 cgatcctgcg atctcccaag agcctgatcg cgtcgaagcc catatcacgc tgagatcaaa
    20221 cgacggcaag tccgagcgca ccaggaagat cacagtccct gtcgagaatc tgatcgaagg
    20281 gtactccagt gccttcccgc ttgtcgtctg gccgtggagc gaatcgggcc gatccgtgga
    20341 cgacctgttc aagcccggcc ccgccgagct gactgttagc atcgtggcta cgcggaaaac
    20401 agataaaggc atcgaacaaa tcggcatctg ggatatcccc acccgccagg tgcagattct
    20461 gcccggtccg catcgagaag gaaaatgaat ggccaatggg tatgatagga ggtaccgggc
    20521 gacgggggca cgggcagatt cggacgtaag aggcatccga tgtcacagag acgcatcctg
    20581 gttgtcgaag atgacgccgc gattcggcaa ggtatcgtcg atgccctgca attcgacggt
    20641 tatgccacgc tcgaagccgc caacggcgac gtggggctgg atatggcccg agccgtggat
    20701 tgcgatctgc tgctgctcga cctggtgctg ccaggggcag acggtttgac tattctgcgg
    20761 gaagtgagga tcacgcgacc cacgctgccg gtgatcattc tcaccgcccg cggcgaagag
    20821 caggaccggg tacagggcct gaaacttgga gcagatgact atgtcgtcaa gccgttccgc
    20881 gtcgcagaac tgctcgcccg cgtggaagcc gtgctgcggc gatctcccga gcggccggcc
    20941 gatctcaggc ttgttgcttt cgccggtggc gtgatcgacc tggcgcggag cgagattcgt
    21001 tttgagaaca acgaccgctg cgagctgtcg gagcgcgagg ttgaactgct gcgttacctg
    21061 gcctgcaaca acggacgggc gatatcgagg gacgagatac tctcgcgcgt ctggcggctc
    21121 gatccgatcg gcatcgcgac gcgaacgatt gacatgcaca tcgcgcggtt gcgagacaag
    21181 ctgcgagacg accctgagga tccaaagatc attctcactg ttcggggcaa ggggtacatg
    21241 ttcgccaact gcggaggagg cggttgatgc gaccctggct gacatggccg gcctttgtcg
    21301 tgtgcattgc cgtgatcctg gcggccatgg gctggcatag tcagaccgtg ctgcaacttg
    21361 accgtgcaga cgcggccgcg attcgccgag ccgcgatcga ggagcgaatc cgcctggccc
    21421 tgtggcggat ggattcggcc ctgacaccgt tggtcgccca ggaaagtacc tggccatact
    21481 acgcgtacgc gtcgttcgcg ccgacggaaa gggcctatac gaatctgctc aacccggtcc
    21541 aaccaggcga gattctgatg gcctccccgc tgctcgaccc gccgtcgcgg catgtgttgg
    21601 tgcattttca gatcaatccg gataacagca tctcctcgcc gcaggtaccg accggcccga
    21661 tgcgccagat tgcggaaggg caatacacca ccgccgacaa gatcgagctc tacacccggc
    21721 gactcgcagc tctccaaccg atcctgagca gggccaacct cctgagcatg ctggaaccgg
    21781 cggagccccc tcctgtcaga attgccgtgc tgccgaccgc ctctcaaccc tcggccgacc
    21841 aggagagcct cgcccagcaa tccgaggttc agtcgttttg gggtctgtcc aaccagcttc
    21901 ccgatcggga tctccggcgg cgattgggtc ggcttgacga gcagatctac acccagcaag
    21961 ccgtcactcc tcagcccgcg gcacaggagc aaggacaagt ctcgggatta tcccagggcg
    22021 gcaaccccag acaagaccgt gctcaacggc cgtcgtcctc cggcaaacag gtgagaagtt
    22081 ccggcaagac aagctggata tcacccgatc aacaggacct gatgaacacc tacgatgccc
    22141 aagcacgggg ctacaactac gacgtcaacg gagagctgat cctgactaac gccggcaatt
    22201 cgagagtaat gcgagggggc gtcaaagagg gatcggcacg gtctttgtgg atcggggaca
    22261 cgctcgtact ggctcgcaga gcaagggtca acgggagcga atacattcag ggctgccggt
    22321 tcgattggcc ggggattcag agctggctgc tcggcgagat ccgggatctg ttgcccgaag
    22381 cccgtctcga accggccgac ctggagctcg accagaccga cacgcgccgg ctggcagctc
    22441 tgccgatccg cctcgtcccg ggcgacatac ccagcccccc ggcacagggc atgtccccgg
    22501 ttcgaatgac gttactcgtc gcgtggtcct gcctgctcct ggcggcggcc gcagtgatca
    22561 tactgctggt gggaacgatg tctctgagcg agcgccgggc agccttcgtc tcggcagtca
    22621 ctcacgagat gcggacgccg ctgaccacgt ttcggatgta caccgagatg ctgaccggtg
    22681 gcatggtgcc cagcgaggaa aaacgacggc gatacctcga tacgttgcgc gtcgaagccg
    22741 agcggctcag ccacctggtt gagaacgtac ttgcttatgc ccgcctggag aagaatcggg
    22801 cgatcagcgc cgctcagaac gtgacccttg ccgagttgat cgcgagagtc gagaaacgcc
    22861 tggccgagcg cgccgcacaa gcgggcatga aactggaggt atcgaggccc gaggacatct
    22921 gctcttcgac tgttcacgtg gatccttccg cagtggaaca aatcctgttt aacctggtgg
    22981 acaacgcctg caagtacgcc gtctcgaccg gtgatcggcg aatccggctg gagacgctgc
    23041 gggacggagg caaggcaacc gtgcgggtcc gcgattacgg gccggggatc cccgagcgcg
    23101 aggcaaggaa gctatttcgt ccgttctgca agtcggcaaa ggacgccgcg aactcatcgc
    23161 ccggcgtggg tctgggattg gccctcagtc gccgactggc gcggcacatg ggaggcgatc
    23221 tgcgactcga tcagagcgtc aacgatggcg cctgcttcgt cctcactcta cccgcaacgg
    23281 tcaactgatt ccccctgctc cagcacccga actcttgacg agccacgggg ttgctcgggc
    23341 tgagcctgtc gaagccatcc ccgtggtctt gccgaggtgg gcgtcgaatc ccctcgctgg
    23401 cagcggaatc cgttctgctg gcatcggaat ctgttctgct ggccccggaa tccggtctgc
    23461 tggccccgga atccattccg gggccttccg cgccgaggat tcccatcctc attctcgccg
    23521 atggcggaat gaactccgcc aggcagggcg gtcctgcttc gcgaccagcg cttcgcagga
    23581 caggcccgga agggattccg ggccgagcga atgtggattc cgggccgagc gaatgtggat
    23641 tccgggccga gcgagcccgg aagggattcc gggccgagcg aatgtggatt ccgggccgag
    23701 cgaatgtgga ttccgggc
//
LOCUS       DAOTPW010000064        23591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2228_length_23591_cov_4.914641, whole genome shotgun sequence.
ACCESSION   DAOTPW010000064 DAOTPW010000000
VERSION     DAOTPW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23591
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2228_length_23591_cov_4.914641"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1389)
                     /locus_tag="P5316_07725"
     CDS             complement(184..1389)
                     /locus_tag="P5316_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR84884.1"
                     /translation="MSHASHNTVSRRDFLRTTTCGAAAIGLGPMASGTLAASTASQPA
                     KTFKKPSLPYGKLGRTDFPVTLISFGAIRIAERLGTRIAKGVIDAGINLIHTSSSYMG
                     GNSIAAIADLFKADKSYREKVFLCLKSFNPDSEKEIDEMLMTLGTDHTDALLTTFENA
                     DPRRLEAIQKQQEALIKKGKLRHTGFVCHGDLHGVMELTIEKAPRFFEVALLAMRMLP
                     LGKSSISDESKRFLKNLKTFRDNGVGILSMKSGAWSALEHGADVFLPHAKAVLEAGCD
                     SVLTSVNRFDQIDMIKTLDLRSRHMTEDEKKAAAEFLDGRRQACLMCARCRKVCPQGL
                     PVSDLMRIRMYNDEYGWPDHAGNEFARLGLDANRLASACGDCTACAQACPIGLAGAEA
                     VRHVAEMFV"
     gene            1607..2956
                     /locus_tag="P5316_07730"
     CDS             1607..2956
                     /locus_tag="P5316_07730"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRR84885.1"
                     /translation="MTGKPWEPAGWRCCPAAQQPQYPDATAYEAALCALKNCPPLVAV
                     GEVDNLRARLAGAGRGEAFILQGGNCAERFLDCRAESIANKLKILLQMSVILTYGVRR
                     PVVRIGRFAGQYAKPRSNETEIADDRELPVYRGDAVNAVEPTPEARAADPQRLVSAYF
                     YAAATLNHIRALIDGGFADLRHPYTWNLSSMENSRNWNEYKAVLDQILDAINFMESFG
                     GARSDVLGRVDFFTSHEGLLLGYEEAMTRKDPDSGRWYNLGAHMLWIGARTRQVDGAH
                     VEYFRGIANPIGLKVDAAMRPEELLALVDVLNPHNDEGRLTLITRLGAGRVEERLPPL
                     IRAVRNAGRNVVWSCDPMHGNTVTLENGRKTRDFSVVLDELRRTFEVHRRQASRLAGV
                     HFELTADDVTECIGGAGDLTSGDLARRYETYCDPRLNCSQSLEMAFLIARLLQSQRF"
     gene            3205..3927
                     /gene="pyrH"
                     /locus_tag="P5316_07735"
     CDS             3205..3927
                     /gene="pyrH"
                     /locus_tag="P5316_07735"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRR84886.1"
                     /translation="MARTKVAFSRVVLKLSGEGLGGRGGAGIDPEQLDYVTREIVAVH
                     RTGVELAIMVGGGNILRGGAFSRKSAIPEATAHYMGMLATVINGLALQEALEDKKVPT
                     RLMSALGVTGLCEPFSRHRCKGHLDKGRVVVLAGGSGRPFFTTDTAAALAAVEIDADA
                     LLKATQVDGVYSADPKKDPRAMFYPKLSYDDVIDKRLAVMDLSAVEMCQRCGVTMVVF
                     NLHKAGNLKRAATGRRVGTIIS"
     gene            4000..6087
                     /locus_tag="P5316_07740"
     CDS             4000..6087
                     /locus_tag="P5316_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84887.1"
                     /translation="MDLIMTSRLAMILLATAACIVPTPWISLPAAAILVLWLPGRSLL
                     CLLPGLESLRTSKWAMVAASAALMPVPLSWAWRLTNARWAIVALVCGLNLVLAVAVRK
                     DIRPREAPPASTSRRYRLLLAAMTIWTSGCVFLSFWLPSQVGNVTITRAHDYVKHHAV
                     MLSLERHPLPLHSCFYRAEADTPYYYYEYYYLLPAALRTMTGNKVSIPLAFGLTSALL
                     AAILIHLTSSLAGSITRDERCSALAAAFVSLVGGFDIVPVLIRAATGGSLVVTLDAWC
                     PVAWRVHNFTTQYFWCPQHVFAVVGLTLAAIWLRLSPRGTWWLLMAPLLSASIFGGSA
                     HLAIVIFPAAAVYAVLFLWRIAREDRTSLKRVLAGIVVIGVLGLALMGRQAWGYKEMS
                     SRYPGGLTTSWERFPFAIFGRLLPAGPLANYADAPWLLIVELGLPALACLLVSGTFWR
                     VLWADPGVKLLILVGLSGAVLMFTFRSEVNAIDYGFRIAPMAAAPLLAGMAGSLLNAD
                     LLRRRVRRIGVGLAVIGASLGLPVGLYEAPLTAVRTIWESRRLMPESGAIRFLRESTP
                     TDAVIQGDPDCRLDLAALTDRRIGASKPNSPHVVVFSPKDPASMRRAYSDVMQAFYTP
                     SSEEAHAKLETWGVRYVFVGLAERKRFGIMPQFNDQRWFEPVYNGDGVTVYRLADLFS
                     QSRPEVITQRGGP"
     gene            6084..6740
                     /locus_tag="P5316_07745"
     CDS             6084..6740
                     /locus_tag="P5316_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84888.1"
                     /translation="MTRNHPAHRCTERFRLCPYAVLFLSLLVVTDAAPSFADSAQSAD
                     QLVEIRGGADETRQNYEWTVINKHSLAIVEVEFPHYHADLFQIIPPAGWEQECTYLVN
                     VGVPDRPGVCIARRKPPNPGIPPGGQHLFAMRLTAKGALVGKGKVTIKFADDRTVIVP
                     GVTLPVPPPPSFKLLPLLGAAIIFGLWVLVRTVRERRRKGQRTEGPVAGSQVEQKPES
                     "
     gene            6923..7558
                     /locus_tag="P5316_07750"
     CDS             6923..7558
                     /locus_tag="P5316_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRR84889.1"
                     /translation="MTTPSDEQLLAEHVEGLPDRFELLVRRHSHELFRFLVRFTGSQA
                     LAEDVVQEAFLQVHLAAASFDRSRRFKPWLFTIAANKARDCLRSRGRKPEVPLDAHVG
                     NADQETGQTFRDFMADNSADPVAGLESMEEREQVRAVLARMPDHLREVLVLSYYHQFP
                     YREIAEILNVPLGTVKSRLHAAVATFGKAYKAAVGKRSVGGPSAPKGLKAV"
     gene            7593..8615
                     /locus_tag="P5316_07755"
     CDS             7593..8615
                     /locus_tag="P5316_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84890.1"
                     /translation="MQDDSQTIESKLLDLQLNRLDPEQAREVEEAVTTSADLAAKSRA
                     LRTLLGLLDAVEVPEPSADLADAVMARIDERTRPIPFKEMTPVSPVEGRRVSGRSVLS
                     LRELIAIAACITLFVGVFVPGYQKAQNIARRNACRWGLRTVSLGMSAYAQQNDGYLPY
                     FGYMPNASWLRTRTPNVPRYSNTRAMFVLLRQGLIPNDDPRVFICPSMPDARPMIARD
                     YQRFDDFAEPANVSYSLLLMNVLKGWRVEKMDPQMVIAADANPLFDGRGGGHRISPYD
                     DNAANSLAHENGAGQNAVYVGGTGGWFTRPTIGVDQDNIYRAGRLTQYTGVERPFGDT
                     DTLLVP"
     gene            complement(8699..9034)
                     /locus_tag="P5316_07760"
     CDS             complement(8699..9034)
                     /locus_tag="P5316_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HRR84891.1"
                     /translation="MSFEDKTIVCSDCGQTFIHSAQDQARYAQRGFTNEPKRCRECRD
                     KRKAQGGSGRSGGASGNRSFNGGRGDRPRRESFPATCAECGAQTTVPFKPVQDRPVYC
                     QDCFRARKR"
     gene            complement(9312..10745)
                     /gene="lpdA"
                     /locus_tag="P5316_07765"
     CDS             complement(9312..10745)
                     /gene="lpdA"
                     /locus_tag="P5316_07765"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRR84892.1"
                     /translation="MSESPKQRESDVVVIGAGPGGYTAAFLAADHGRKVTLIDAGEQP
                     GGVCLNRGCIPSKTLLHMARLLSEAREAADRGVSFQRASVDLEKLRAWKNEVVSRNAS
                     GLRQLAQARRVEMVRARARFLGSRVLRLTDASGDDLCLGFNHAIIATGASPARLPGLT
                     VESDRIMDSTAALELPEIPGSLLVIGGGYIGLEIGTVYAALGSRVTVVEMTADLLPGA
                     DRDLVRILHNRLKKSFANIMLKSKVVSLAEAGQGISATIATEGADSPTQQVFDRVLIA
                     VGRLPNSSELDLELAGVEVDRQGFIRVSQQLRTGNERAFAIGDVAGGLMLAHKAAHEA
                     RVAVEVLAGRDARSVPAVVPCVAFTDPEVAWVGLTETEAAAKGIPFEAARFPWAACGR
                     AASIGRTDGLTKILVSPDDKRILGVGIVGTGASELVAEGVLAIEMKATAREVARCIHA
                     HPTLSETVGEAAESALGITPHILRPRR"
     gene            10972..11421
                     /locus_tag="P5316_07770"
     CDS             10972..11421
                     /locus_tag="P5316_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84893.1"
                     /translation="MSRAGREPAEDVKRRIVAACETAGLKVKSARMYLRKAQRDRIVL
                     VAASPVDWRAQRPMVTILTTVDVCGEWSGEVDVRCSAGEDEPVVKFWDIPVMQGRNTV
                     PMHELPRQLCETMREREQVVAAMRLGVTGPYTFERSRWQKPGDLLRA"
     gene            11601..13058
                     /locus_tag="P5316_07775"
     CDS             11601..13058
                     /locus_tag="P5316_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRR84894.1"
                     /translation="MDVIWLSRVQFALTIMFHYLFPPLTIGLGAMLVILEGAWLKTGD
                     AGYKSAAQFWTKIFALNFAMGVVTGIVMEFQFGTNWATYSRYVGDVFGSALAAEGIFA
                     FFLESGFLAVLVFGWDRVSPRMHFFSALMVALGSIFSSIWIVVANSWQQTPAGFHLVE
                     QTIGGRTQIRAEITDFWAVVFNPSTVERLVHVWIGAFILGAFFVTSITAYYILRKRHE
                     TFARRSFAAALGVTLVASLAALVSGHSQARNVYHTQPAKMAAFEGHFQTARGVPLYLF
                     GFPDEQTQQVRAGLKIPGMLSFLMHNDAKAEVTGLDRFPRDEWPPVAASFHCYHIMVA
                     LGMFFIGITVLAAVLWWRGTLFNKKWLMWVFVFAVIGPYIANELGWAAAEIGRQPWIV
                     YGLLKTADAASPSVGAAEVLGSIVMFSLIYLILFGIWLYLLNAKIKAGPEEAAGDTAD
                     GKGFFDTAARFAGHEKGVSMTESEPGRSVDTGGRE"
     gene            13063..14094
                     /gene="cydB"
                     /locus_tag="P5316_07780"
     CDS             13063..14094
                     /gene="cydB"
                     /locus_tag="P5316_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRR84895.1"
                     /translation="MELTHDTLCQIWFVLLGVLMTGYAILDGFDLGVGILHLAARDDR
                     ERRVLMNSIGPLWDGNEVWLVTFGGAMFAAFPHAYATVFSGLYVAFMALLLALIFRAV
                     SLEFRGKTASATWRRIWDFGFFAGSLLAALLFGVAVGNAMIGMPIGPDMEFVGSFLDL
                     LRPYPLLIGLFTVAMFAMHGALYLYLKTEGRLQERIASWMWHTFGVFFVLYLLTTIFT
                     LATVPDAIRNFAAHPWAWGVVVLNVLAIANIPRAIYLRRPGYAFVSSCCTIAAFIFLF
                     SIALSPNLVTSSIDPRYSLTIYNAASSARTLTIMLIIAVIGMPFVLAYTAVIYWVFRG
                     KVQLGRHSY"
     gene            15194..16648
                     /locus_tag="P5316_07785"
     CDS             15194..16648
                     /locus_tag="P5316_07785"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRR84896.1"
                     /translation="MKRPMPGLGWFGSTLLVSSIAVAANDVGFYQIGSASVVITPKVA
                     HDAPPVWLAGYGQGRQAEGVHDDIYARAIFIHDGQFGLAIVACDLIGLFHDEVAKVRK
                     ELGRLQLSPSIDYVLVSSTHTHAGPDTLGLWGPVGRTGITPGYLDEVRTACVDAVRRA
                     HRNARRGTLRIGTADVNRLAELIGDSRLPKVIDSQLTVIQATNDGGEMIVTLVNMPCH
                     PEVLGSKNKQLSSDFPSTLRQYLETKFGGLAVYNSGAVGGLLSPREPKSDPFTNEPLP
                     EDEIGRMIAYGRIIGRIAEGAIDQAQPLRGPIRAISREVQIPLWNPFYRLGGSIGVLQ
                     RPMLDADGRPVKMQQAATATAAAGAPAEGDLYLKTEIGLIQIGSLQIAAVPGELYPEL
                     ALGRFQQPQEAAADFPNAPLEPAIYPLMTGRFKMVIGLANDEIGYIIPKSQWDWSSPY
                     AYGRKERQYGEINSCGPEAATRLMAAWQSLVAQR"
     gene            complement(16729..18231)
                     /locus_tag="P5316_07790"
     CDS             complement(16729..18231)
                     /locus_tag="P5316_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRR84897.1"
                     /translation="MLLTSFLDTADRCAGKTAVLDETRQLTYNQLVSRAAALARMVTS
                     ASRGANVGILLPSSTDAVAAFYAVLWAGRVVVPLNVLLNPSEIKTIIQDAGVDLILSC
                     SALEKVVNALPAQAVFLDRLPQEPPPSPSGPPRFPAVPESKPDDLAVILYTSGTTAMP
                     RGVCLSHNNLHSNASATVRHVLLRDDDRFLGVLPLSHSFGLLATLILPTLFGATVEYQ
                     TRFQPVEVLRALAGGGISVFMAAASMFAALARVKNVSSEMLRGLRIAVSGGEALPDAV
                     FKDCRTRLDFTILQGYGLTETSPVVSVNQPWAHRPGTIGLPIPDVEVSIRDEQGRALP
                     SGEQGEICVRGPNVMQGYLNRPQETAAVLMPDGWLRTGDLGRQDADGYTMITGRKKEL
                     IIVAGENVFPREIENVLDDHPAVSQSAVIGVPDPRRGEVVVGFVVPKDGAQVSAIELR
                     EYCRRHLASFKVPRQIIIHRDLPRGPTGKIAKRLLPTVTGVADMLMSSKP"
     gene            18688..19011
                     /locus_tag="P5316_07795"
     CDS             18688..19011
                     /locus_tag="P5316_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84898.1"
                     /translation="MRVRALLFEKPTGVFLWVAVPAMLFFSIAPLPAAQQPDAEPPVP
                     KGFGSTEEVKEAIGQGKVRLVNLFIPLPENVEVINNVEYGRVGERSLQLDLYQPKGLV
                     SRSRR"
     gene            19008..19742
                     /locus_tag="P5316_07800"
     CDS             19008..19742
                     /locus_tag="P5316_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR84899.1"
                     /translation="MILIHGGGWKSGNRQIYRYYCIKLAEKGYVTATISYRLSEEAPY
                     PAAVQDAKCAVRWLRANARKHHVDPDKIAVLGGSAGGHLAMMVGYSSGVKALEGNGGN
                     AGVSSAVQAAIDFYGPVDLTADEAKKAGAVIDFMGGKRYNEAEAQYREASPITHLAKG
                     APPTLILHGTIDDTVPIAQADLLARKLKELGIPYEYERLEGWPHTMDLSEDVNRRCLW
                     RVERVLVKHLPVPPGALPPASSTAAE"
     gene            19774..20757
                     /locus_tag="P5316_07805"
     CDS             19774..20757
                     /locus_tag="P5316_07805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR84900.1"
                     /translation="MKRRDFLKATTLSAAGAASETLWLGQAGTAMSQTTPSSNPAVLP
                     RRDYGRTGVQLSIIGLGGIVLAGLEQQRADRIVAEAVERGLNYFDVAPTYGDAELKLG
                     PALAPYRKRAFLACKTTERRREGAASELRRSLERLRTDYLDLYRLHALTDVRKDVDAA
                     FAEGGAMEVFIEAKKSGRVRHIGFSAHSVEAALAAMERYEFDSILYPVNFATDYANDF
                     SRQVIETARRKGVAVLALKAMARQAWQEHDSRRAAYNKCWYEPLTDPREAELGVRYTL
                     SRPVTAAVPPGEESLFRLAMDVAMRFQPIRPSEAEELKTLTATLKPIFPKV"
     gene            20776..22908
                     /locus_tag="P5316_07810"
     CDS             20776..22908
                     /locus_tag="P5316_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84901.1"
                     /translation="MAAFSRVACFVVLATLLPACSPAAQVCEDVPFRQVRAIHFKNEV
                     DLSDYELRTVRVDRDGKVLINTNRGLLKGYDGRLVRYRELAGISELDHYDLELLKGKF
                     VFLTDKMLLPLHGAGVDFHHNTESQWTRLAAAASGHYLLLSKAKIAEIAGGKNAQIDN
                     PGFSEVILCPFDRMFVLYATDKLARYQGGRLIPLPAPPARITGVVPASNGELKVATDQ
                     GLFTVTDKSVVADLRPLPWQDLTCITRDADGRLWIGSTRGAFSLDHAGEINYYAGRRW
                     LQDDQVIDIHVDMRNDVYVLNRRGLSQLRFEMMTLADKAAIELRNLRLNHIRYGLVSD
                     AELVDADYAHLRLHDTDNDGLWSSLYLASEAFRFAVTGAEDARENAIDGFDAIERLVT
                     ITSIPGFQARTFELEGYKRSDPERWRTRPQHDFEWKGHTSSDEIVGTFFFHSVFYDTV
                     ARDDPALRERVASVVRAITSHILDHNLYLVDVDGKPTTWGRWNPEYVNTPAVGGDRRL
                     NSIEILSFLQLAYHVTGSQRFKDAFYDLVRKHGYADNTINYLPDPRGEWNHSDDELYW
                     LSYYNLIKHCFDDKLKATFLKSVRAHKEATHRKRNPVWNFIFGGVTGEAIDLEGSIFV
                     LRRFPLDNRNWRMQNSHRLDIRIDARPGVEPEAAAVLPPDERRIQKWNGNEMGLDGGG
                     DGNSPESGAEYLLPYWMGRYFGFITAPR"
     gene            complement(23018..>23591)
                     /locus_tag="P5316_07815"
     CDS             complement(23018..>23591)
                     /locus_tag="P5316_07815"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HRR84902.1"
                     /translation="ETGQKLLAIVDPYSYRKRLKIPKLILLGTNDRYWTLDALNLYYD
                     GLEGEKYILYVPNVGHGLNDMVRVVSSVSAFALKAAGKLDFPKLTWDMKEDGDTLLLT
                     IWSDVKPSSVSAWVAESPTKDFRDAKWRPEAIDPAGEEYEYRLKQPEKGFAAVFGEAV
                     YEVKGRMIYLSTNVRIVGTQPALPVSGPTP"
BASE COUNT         4308 a         7308 c         7359 g         4616 t
ORIGIN      
        1 acgaataccg ccaggcgcgg ccgcccggca cgggcacgta gggcaggtca cagcgaagcg
       61 gcgacctgcc tttcccgctg tgccgcacga aacgaaccca ccgcgagcaa gtcagcggtg
      121 ttcagttctc actggtaggc cgctattcgc cggcccccag tccccgccac ggcccatgag
      181 tggctacaca aacatctccg ccacgtgacg gacagcttcg gcgccggcca ggccgatggg
      241 gcaggcctga gcacacgcgg tgcagtcgcc gcaggccgag gccagacgat tcgcgtcgag
      301 accgagcctc gcgaactcat tgccggcatg gtccggccag ccatactcgt cgttgtacat
      361 ccgaatccgc atcagatcgc tcaccggaag cccctgaggg cagacctttc gacaacgggc
      421 gcacatcagg caggcctggc ggcggccgtc gaggaactcg gccgccgcct tcttctcatc
      481 ctccgtcatg tgccgcgacc tcaggtccag cgttttgatc atgtcgatct gatcgaagcg
      541 gttgacactg gtcaatacgc tgtcacaacc ggcttcgaga acggccttgg cgtgaggcag
      601 aaagacgtcc gcgccatgtt cgagcgcgct ccaggcgccg ctcttcatcg agaggatgcc
      661 gacgccgtta tcgcggaagg tcttgaggtt tttcagaaac cgcttgcttt catcggagat
      721 gctgctcttg cccagcggca gcatccgcat tgccagaagc gccacctcaa agaacctcgg
      781 ggctttctcg atggtcaatt ccatgacccc gtgcaggtcg ccgtggcaaa cgaaaccggt
      841 gtgcctgagc ttgcccttct tgatcagcgc ctcctgctgc ttctgaatgg cctccagccg
      901 gcgggggtcg gcgttctcga acgtggtcag cagggcgtcg gtatgatccg tgcccagcgt
      961 catgagcatt tcgtcgattt ccttctcgct gtcggggttg aagctcttga ggcagagaaa
     1021 gaccttctcc cggtagctct tgtccgcttt gaagaggtcc gcgatggcgg cgatgctgtt
     1081 gcctcccatg taactggagg atgtgtgtat caggttgatt cctgcgtcaa tcacgccttt
     1141 cgcaatgcgc gtgcccagtc tctcggcaat gcggatggcc ccgaagctga tcagcgtcac
     1201 cgggaaatcc gtccggccca gcttgccgta aggcaacgaa ggtttcttga aggtcttggc
     1261 cggctgcgag gctgtcgatg cggccagagt ccccgaagcc atcggtccca gcccgatggc
     1321 cgcggcgcca caggtggttg ttcggaggaa atcgcgccgg ctcacggtat tgtgcgatgc
     1381 gtggctcatg gttacccctt tccaaaatca gttcgcgacg cggtcagtat aaaccggcag
     1441 agctgcgatt caaaaccaac cacccggccg tgttgcgttg tcccgggatg gcgagagcga
     1501 tgacgacggg tacaataggt ctggaccgtg gcccggcgtc cggtttcggg gcatccgggc
     1561 gatatcggca cagcaaggtg tgttcagtct ctttcaggaa tccgcaatga ccggcaagcc
     1621 atgggagccg gccgggtggc gttgctgtcc ggccgctcag caaccacagt atcctgatgc
     1681 aacagcatat gaagcggcat tgtgcgcgct gaaaaactgc cctccgctcg ttgcggttgg
     1741 tgaagtcgac aacctccgag cccggctggc cggtgccggc cgcggcgagg cattcattct
     1801 tcagggcggc aactgcgcgg agcggttcct tgactgccgg gccgagagta ttgccaataa
     1861 gctcaaaatc ctgctccaga tgagcgtcat tctcacctac ggcgtccgca gacccgtggt
     1921 gcgcatcggc cgtttcgccg ggcagtatgc caagccccgg tccaatgaga ctgagatcgc
     1981 ggacgaccgt gagttgcccg tctaccgcgg cgatgccgtc aacgctgtcg agccgacgcc
     2041 cgaggctcgc gccgccgacc cgcagcgact cgtctccgcc tacttctacg ccgccgcgac
     2101 gctcaatcac atccgggcgc tgatcgacgg aggctttgcg gatctgcgac acccctatac
     2161 ctggaacctc agctcgatgg agaacagccg caactggaac gagtacaagg ccgtcctcga
     2221 ccagattctc gatgcgatca acttcatgga atcgttcggc ggggcacggt ccgacgttct
     2281 gggccgagtc gatttcttca cctcgcacga agggcttctg ctgggatatg aggaagcgat
     2341 gacccgcaag gaccccgact cgggacgctg gtacaacctg ggagcgcata tgctgtggat
     2401 cggggcccgg acccgtcagg tcgacggagc tcatgtcgag tacttccgcg ggatcgccaa
     2461 cccgatcggc ttgaaagtag atgcggccat gcggccggaa gagctccttg ccctggtgga
     2521 cgtgttgaac ccgcacaacg acgaaggacg cttgaccctg atcacgcgat tgggggccgg
     2581 gcgagtcgag gaacggctgc cgccgctgat tcgggcggtt cggaacgccg gccggaacgt
     2641 ggtttggagt tgcgacccga tgcacggcaa caccgtgacg ttggagaacg gaaggaagac
     2701 tcgcgatttc tcggtggtgc tcgacgagtt gcggaggacg ttcgaagtcc accggcgcca
     2761 ggcaagccgc ctggccggcg tacattttga actgaccgcc gacgacgtca ccgagtgtat
     2821 cggcggggcg ggcgatttga ccagcggcga cctcgcgcga cgctacgaga cctattgcga
     2881 tccgcggttg aactgctcgc agagcctgga aatggcgttc ctgatcgccc ggctgctcca
     2941 atctcaacgg ttctgacttg ccggatgccg atgatgtgat taaggtcacg gccgttggac
     3001 accgaggttc gatccccggt ctccagtgga acgaggtctt gggagtgatg tgctttggcc
     3061 gctcggcccg gaatcccttc cgggccgttc tggccggccg aatccgttcg gcgttcactg
     3121 ctcatgagga tgagagtcct cggtgcaaac cgccccagaa aggattctgg ggcgagcggt
     3181 acgcggtctt gggagtgatg tattgtggcc aggacgaagg tcgctttttc cagagtggtg
     3241 ctcaagctga gtggcgaggg cctgggcggg cgcggcggcg ccggcatcga tccggaacaa
     3301 ttggattacg tgacccgcga aatcgttgcc gttcatcgca cgggtgtcga gttggcgatc
     3361 atggtcggcg ggggcaacat tcttcgcggc ggggcttttt ccaggaagtc cgccatcccc
     3421 gaggcaaccg ctcattacat ggggatgctg gccacggtga tcaacggtct cgctttgcag
     3481 gaggccctgg aggacaaaaa ggtgccgacc cgcttgatgt ccgccctggg cgtgacgggc
     3541 ttgtgcgaac cgttctcgcg gcatcgctgc aaggggcatc tcgacaaggg ccgcgtggtt
     3601 gttctggccg gcggcagtgg acgcccgttc ttcaccaccg acacggccgc cgctctcgcg
     3661 gccgtcgaga tcgacgccga tgcactgctg aaggcgaccc aggtcgacgg cgtctacagc
     3721 gccgatccca agaaagatcc tcgggccatg ttctatccga agctcagcta cgacgacgtc
     3781 atcgacaagc ggctcgcggt catggatctc agcgcggtgg aaatgtgcca gcgctgtggc
     3841 gtcaccatgg tggtgttcaa tctgcacaag gccggcaatc tcaagcgggc ggccacaggc
     3901 cggcgcgtgg ggacgattat ctcgtgaccg gcgtcgtcca cccggcaaaa tggcggcagc
     3961 cgatgcccaa atcgccttga tggccgacgg gttgggtgta tggacctgat catgaccagc
     4021 cggctcgcca tgattcttct cgcgaccgca gcctgcatcg tgccgactcc gtggatctcg
     4081 ctgcctgctg ccgccatatt ggtgctgtgg ctgccggggc ggtcgctcct ctgcctgttg
     4141 cccggcctgg aatcgcttcg cacttcaaag tgggcaatgg tggcggcatc tgcggctctc
     4201 atgccggttc ctctgagctg ggcttggcgc ttgaccaatg cccgatgggc gatcgtggcg
     4261 ctcgtctgcg gcctcaatct ggtcctggcg gtggccgttc ggaaagacat ccgcccccgg
     4321 gaagctccac cggcgtccac gtcccgtcgc tatcgcctgc tgctcgcggc aatgacgatc
     4381 tggacgtcgg gttgcgtgtt cctctcgttc tggctgccca gtcaggtggg aaacgtcacc
     4441 atcacccgtg cccacgacta cgtcaaacac cacgccgtca tgctctcgct cgagcggcat
     4501 ccgcttccat tgcacagttg tttctatagg gccgaagccg acacgccgta ctattactat
     4561 gagtactact acctcctgcc ggccgcgctc cggacgatga ccggtaacaa ggtatcgatt
     4621 cccctcgcct ttggcctgac gtccgcgctt ctcgcggcaa ttctgatcca tctcacctct
     4681 tcgctggccg gcagtatcac aagggacgag agatgcagcg cgctcgcggc tgcgttcgtc
     4741 agcttggtcg gcggcttcga tatcgtcccc gtactgatca gggcggcgac cggcggaagc
     4801 ttggtggtca cgttggacgc ctggtgtccg gtggcctggc gggtccacaa tttcactacg
     4861 cagtacttct ggtgcccgca acacgtcttc gccgtcgtcg ggttgacgct ggcggcaatc
     4921 tggctgaggc tgtcgccgcg cggcacgtgg tggctcctga tggcgccgct gttgtcggcg
     4981 tcgatctttg gcgggagtgc tcatctggcc atcgtgatct tcccggcggc ggccgtctac
     5041 gccgttctct tcctgtggcg gattgcccgc gaggaccgca cgagcctcaa gcgggtgctg
     5101 gcgggaatcg tggtaatcgg cgtgcttggt ctggcattga tgggccggca ggcatggggc
     5161 tacaaggaga tgagcagccg ttaccccggc gggctgacaa cctcctggga aagattcccc
     5221 tttgcgatct tcggtcgctt gctgccggcg gggccgctgg ccaactatgc cgacgcgcct
     5281 tggctgctga tcgtcgagct tgggctgccg gcactcgcct gcctcttggt gtcagggacc
     5341 ttctggcgtg tcctgtgggc ggatccgggc gtgaagctgc ttatcctggt tgggctctcg
     5401 ggagccgtac tgatgttcac ctttcgcagc gaggtcaatg ccatcgacta tggttttcgg
     5461 atcgcaccga tggctgcggc acctttgctg gccggcatgg ccggatccct gctcaacgcc
     5521 gatcttctgc gtcggcgcgt tcggcggatc ggcgttggtc tggcggtcat cggcgcgtcg
     5581 ctgggtttac cggtgggttt gtacgaagca ccgttgaccg cagtcaggac gatctgggag
     5641 tcacgacgac tcatgcctga gtccggggcg atccgtttct tgcgggagtc gacgccgacc
     5701 gatgccgtca tccagggcga ccccgattgc aggctggatc tggcggcctt gaccgaccga
     5761 cgcatcggcg cgtcgaagcc gaattcccct catgtggtcg tgttcagccc caaggatccc
     5821 gcgagcatga ggcgggctta ttctgatgtc atgcaagcct tctacacgcc gtccagcgag
     5881 gaagcgcacg cgaaacttga aacgtggggc gtccgatacg tcttcgtcgg cttggccgag
     5941 cggaagcgtt tcgggatcat gccgcaattc aatgaccaac gttggttcga gccggtgtat
     6001 aatggcgacg gtgtcaccgt ctatcggctt gcggatttgt tttcgcaaag ccggccggag
     6061 gtgattaccc agaggggcgg tccatgacgc gaaaccatcc tgctcatcgg tgcaccgagc
     6121 gattccggct ctgcccatat gcagttctgt tcctgtcgtt gctcgtcgtg acggatgcgg
     6181 caccctcctt cgccgactcc gcgcagtcag ccgaccagct tgttgaaatc aggggcggcg
     6241 ccgacgaaac acggcaaaac tacgaatgga cggtgatcaa caaacactcg cttgcgatcg
     6301 ttgaggttga attcccgcac tatcacgccg acctcttcca gatcatccct ccggccgggt
     6361 gggaacagga atgcacctac ttggtgaacg tcggcgttcc tgacaggccc ggagtctgta
     6421 tcgccagacg caagcctcct aaccccggta tcccgcccgg cgggcagcac ctttttgcca
     6481 tgcgactcac cgccaaggga gccttggtcg gtaaagggaa agtcaccatc aagtttgccg
     6541 acgacaggac ggtcatcgtg cccggcgtta cgctgcctgt gcccccgccc cccagcttca
     6601 agctcttgcc ccttcttggt gcggcgatca tttttgggct ttgggtgctc gtccgtaccg
     6661 tgcgcgagcg gcggcgaaaa ggacaacgga ccgaggggcc ggttgccggc tcgcaagtgg
     6721 aacaaaagcc ggaaagctga cccaagatct tttcgggggc cgataatggt cgaacgggtt
     6781 caaccctctg ttgccacgaa tgttatggcg gcacgtctgc tgcctcactt tgaccgtcga
     6841 cggacacgga tctattatga ggtcgttgtc caaccgtgcg gtgaacaaag cgcaagtcca
     6901 atcgtaagga actgttgaag cggtgacgac gccttcggac gagcagttgc tggccgaaca
     6961 tgttgaaggc ctgcccgacc ggttcgagct gctggtccgg cggcatagtc atgagctgtt
     7021 tcgctttctg gtccggttta ccggtagcca ggcgctggcg gaggacgtcg ttcaggaagc
     7081 gttcctccag gtgcatctgg cagccgcaag cttcgaccgg tctcggcgat tcaagccgtg
     7141 gctgttcacc atcgcggcaa ataaggctcg cgactgtctc aggagccgag ggcgaaaacc
     7201 tgaagtgccg ctcgatgcac acgtcggcaa tgccgaccag gaaaccgggc agactttccg
     7261 ggacttcatg gccgataatt cggccgaccc ggtggccggg cttgaaagca tggaagaacg
     7321 cgagcaagtt cgcgcggttc tggcaaggat gccggatcac cttcgggagg tgctggtgct
     7381 gagctattac catcagtttc cgtaccggga gatcgcggag atccttaatg tcccgttggg
     7441 gaccgtcaaa agccggttgc acgccgcggt ggccactttc ggaaaagcct acaaggccgc
     7501 ggtaggaaag cgatcggtcg gcgggccttc cgccccaaaa ggcttaaagg ctgtttgacg
     7561 atggtcggac gggcaggagc acgttgagga cgatgcaaga cgactcgcag acaattgaat
     7621 cgaagctgct ggaccttcag ttgaatcggc tcgatccgga gcaggcccgg gaggttgaag
     7681 aggccgtgac aacgtcggcc gacctggcgg ccaaaagccg ggccttgcgt accctcctgg
     7741 gactcctcga tgccgtggag gtccccgagc cgtccgccga cttggcggat gcggttatgg
     7801 cccgaatcga cgaacgcacc aggccgattc ctttcaagga aatgaccccc gtatcgcctg
     7861 tggaggggcg acgtgtttcg ggccggtcgg tgctgtcact gcgagaactg atcgctatcg
     7921 ctgcctgcat cacccttttc gtgggagtat tcgtgcccgg ataccagaag gctcagaaca
     7981 tcgcccggcg gaacgcatgt cgctggggac tgcgcaccgt gtccttgggc atgagcgcgt
     8041 acgcccagca gaacgacggc tatcttccat atttcgggta tatgcccaac gcatcgtggc
     8101 tccgcacacg cacgcccaac gtgcctcgct actccaatac cagggccatg ttcgttctgc
     8161 tccggcaggg gcttattccc aacgacgatc cgcgcgtgtt catctgcccg agtatgcccg
     8221 acgcacgccc gatgatcgcc cgggattatc agcggttcga cgatttcgcc gagcccgcaa
     8281 acgtctccta cagcctgttg ctcatgaatg tcttgaaggg gtggcgggtc gagaagatgg
     8341 atccgcagat ggtgatcgcc gcggacgcca atccgttgtt cgacgggcgc ggaggcggtc
     8401 atcgcatcag cccgtatgat gataatgctg ccaactcgct ggcccacgag aacggcgccg
     8461 gtcagaacgc ggtctacgtg ggtgggacgg gcggctggtt cacccggcct acgattggtg
     8521 tcgatcagga caacatctac cgtgccggtc ggcttaccca atacacgggc gtcgagagac
     8581 ccttcggcga caccgacacc ctcttggtgc cgtgatccgc acgggcgcgc agaccctccc
     8641 ccccgtccgc cttgtgtatc gaaaccgatt caagcggctt taccggcaaa agcggcgctt
     8701 agcgttttct cgcccggaag caatcctggc agtataccgg ccgatcctgg accggcttga
     8761 atgggacggt tgtctgagcc ccacattcgg cacaagtggc cgggaaagac tcccgccgag
     8821 gacgatcccc gcggccgccg ttgaagctcc ggttcccgct ggcgccgccg gaacgcccgc
     8881 tgccaccctg ggccttgcgc ttgtcacgac attcgcggca gcgcttgggc tcattggtga
     8941 agccacgctg ggcgtagcgt gcctggtcct gggcgctgtg gatgaacgtc tggccgcagt
     9001 ccgagcacac gatcgttttg tcttcgaacg acatggtgga gttgcctccg tctagaggag
     9061 acggaccatt cctgaatggc cgccgaacct gattgaagcg ggaatcccgg gtaggagggg
     9121 aaccatttga atacctgcaa agggttggtc ggcgtctttc gccgcccctc aaggccgagt
     9181 ggcccgcagt ccaagagcgg tgaacccatt tcaccggcag atcgtaagta tgcccgaaaa
     9241 acctccgccg gtcaactcgc ggcgatgccc gaccttggca gggtttcgag ttgacgtaag
     9301 actcctccgg ctcaccgccg aggccggaga atatggggcg taattccgag agctgattcg
     9361 gcagcctctc cgactgtctc cgacagggtg gggtgggcgt ggatacagcg ggcgacctcc
     9421 cgggcagtgg ccttcatttc aatggccagg acaccctcgg ccaccagttc actggcacca
     9481 gtgccgacga tgccgacgcc caggatgcgt ttgtcatccg gggatacgag gatcttggtg
     9541 aggccgtcgg ttcggccgat gcttgcggcc cggccacacg ccgcccacgg aaatctggcc
     9601 gcttcaaaag gaatgccctt ggcagcggcc tcggtctcgg tcagcccgac ccaggccacc
     9661 tccggatcgg tgaacgccac gcacggcacg accgcaggga cagaacgagc atccctcccg
     9721 gcgaggactt cgacggcgac gcgggcttca tgagcagcct tgtgggccag catgagaccg
     9781 ccggccacgt cgccgatggc aaacgctcgc tcattcccgg tccgcagttg ttggctgacc
     9841 cggatgaagc cctgacggtc cacctcaacg ccggcaagct ccaaatcaag ctcgctggaa
     9901 ttcggcaacc tgccgacggc gatcaggacc cgatcgaaaa cctgttgagt gggcgaatcg
     9961 gccccctcgg tcgcgatcgt ggcggagatt ccctgaccgg cctcggcgag agatacgacc
    10021 ttcgatttca gcatgatgtt cgcaaagctc tttttcaagc gattatgcag gatgcgcacc
    10081 aggtcgcgat cggcaccggg cagcagatcc gcggtcattt ctaccacggt gactcggctg
    10141 cccaacgcgg cgtaaacggt gccgatttcc agaccgatgt agcccccgcc aatgaccaga
    10201 agcgaacccg ggatttccgg caactccaga gcggcggtcg agtccatgat ccgatcgctt
    10261 tcaacggtca gtcccggcag tctcgcggga cttgcccccg tggcgatgat cgcgtggttg
    10321 aacccgaggc aaaggtcgtc gccggaggcg tccgtgagcc gaagcacgcg ggaaccgaga
    10381 aaccgagcgc gggcccgcac catttccacc cttctcgcct gcgccagttg ccgcaggccc
    10441 gaggcgtttc gggagacgac ctcgttcttc caggcacgca gcttctccag atcgacgctt
    10501 gcgcgctgaa aagacacgcc gcgatcggcc gcctcgcggg cctcggagag aagccgcgcc
    10561 atgtgcagaa gtgttttgga gggaatgcag ccacggttca aacagactcc gccgggttgc
    10621 tcgccggcat cgatcagcgt cacctttcga ccgtggtcgg cggccaggaa cgcggccgtg
    10681 taaccgcccg gtccggcccc gatgaccacc acgtcgctct cacgctgctt tgggctttcg
    10741 ctcatctctg ctccgtttcc gttcagcgca cactgcgtcg ggccttttac gtgccggaaa
    10801 ttcgcctcaa tggcggaacg ctcaacactg ccgtgccgga tattatcgga acatcaaaat
    10861 catttgtgaa gcacacttgt ggccccgcaa ctgtttcacc ataatggcgc cagggaagaa
    10921 gcttggcggt tgcaggcgag attgggtcca ttccggtttg cagtgtgagc catgagcaga
    10981 gcaggacgcg aacctgccga agacgtcaag cgacgcatcg ttgccgcttg cgaaacggcg
    11041 ggcctcaaag tgaaatcggc ccggatgtac cttcgcaagg ctcagcggga tcgaattgtt
    11101 ctggtcgccg cgtcgccggt ggactggagg gcccaacgcc cgatggtcac gatcctgacc
    11161 acggttgatg tgtgcggcga gtggtccggt gaagtcgacg tccgctgttc agccggcgag
    11221 gacgaaccgg tcgtcaagtt ctgggacatt ccggttatgc aggggcggaa taccgtgccg
    11281 atgcatgaat tgccccgcca gctttgcgaa acgatgcgag agcgcgaaca ggtcgtggcc
    11341 gcgatgcgac tcggtgtcac cggcccatac actttcgagc ggtcccggtg gcagaaacca
    11401 ggcgatctgc ttcgggcgtg atccgcatcc ccaacccgac aattgcacgg tgagtcgtca
    11461 acttcctctt ttgcaccacg ccgcttgccc tgaacgatcg ctaaaaggcc tttacaccca
    11521 acccattccc gctattctgt tgtcgtggtt cagcgtctga aaaatgaaaa ctgagaactg
    11581 atcactcaca actctcttcc atggacgtca tctggctatc tcgtgtccaa ttcgccctga
    11641 cgatcatgtt tcactatctc ttcccgcccc tgaccatcgg gctgggagcg atgctggtca
    11701 ttctcgaagg agcgtggctc aagacggggg atgccggcta caagtccgcg gcccaattct
    11761 ggacgaagat cttcgctctc aatttcgcga tgggcgtggt caccggcatt gtcatggagt
    11821 ttcagttcgg aaccaactgg gcgacatact cgcgttatgt cggcgatgtc tttgggtccg
    11881 ctctggccgc tgagggcatt ttcgcgttct tcctcgaatc cggtttcctc gccgtcctgg
    11941 tgttcggctg ggatcgcgtc tcgccgcgga tgcatttctt ctccgcgctc atggtggcgc
    12001 tgggctcgat cttctcctcc atttggatcg tcgtcgccaa ttcctggcag cagacccccg
    12061 ccgggtttca cctcgtcgaa cagaccatcg gcggccggac acagatccgc gccgaaatca
    12121 cggatttctg ggccgtcgtt ttcaatccct cgaccgttga acgactggtc cacgtgtgga
    12181 ttggcgcgtt tatccttggt gctttcttcg taacgagcat cacggcgtat tacatcctca
    12241 ggaaacgcca cgagacgttt gcccgtcgat cctttgccgc tgccctgggc gtgaccctcg
    12301 ttgcgtctct ggcagctctg gtttcgggcc attcccaagc caggaacgtg tatcacactc
    12361 aacccgccaa gatggccgca ttcgaggggc atttccagac cgccagggga gtcccgctct
    12421 atctgtttgg tttcccggat gagcagacgc agcaggtgcg tgcgggtctc aagatccccg
    12481 gcatgctgag cttcctcatg cacaacgatg ccaaagccga ggtgaccggc ctggaccgat
    12541 tccccagaga cgaatggccg ccggtggccg cgtcgttcca ctgctaccat atcatggtcg
    12601 cccttggcat gttcttcatc ggcatcaccg tgttggcggc cgtcctgtgg tggcgaggga
    12661 cgctgttcaa caagaagtgg ctgatgtggg ttttcgtgtt cgccgtcatc gggccgtaca
    12721 tcgcgaatga gctgggctgg gcagctgccg aaatcggccg gcaaccctgg atcgtgtacg
    12781 gactgctcaa gacggccgac gcggcctccc cctcggttgg tgccgctgaa gtacttgggt
    12841 caatcgtcat gttcagcctg atctacctga tcctgttcgg catctggctg tatctgctca
    12901 atgccaagat caaagccggc ccggaggagg cggcaggtga cacggccgac ggcaagggct
    12961 tcttcgacac ggccgcacgg ttcgccgggc acgaaaaagg cgtatcgatg accgagtctg
    13021 agccaggacg ctccgtagat accggaggcc gggaataagg ccatggaact cacccacgac
    13081 acactgtgcc agatctggtt cgtgctcctg ggcgtgctca tgaccgggta tgccatcctc
    13141 gacggcttcg atctgggcgt gggcattctg caccttgccg cccgcgacga ccgcgaacgc
    13201 cgcgttttga tgaactcgat cggaccgctc tgggacggca acgaagtatg gctggtcacc
    13261 ttcggcgggg cgatgttcgc ggcgttcccc catgcttacg ccacagtctt ctcgggcttg
    13321 tacgtcgcct tcatggccct gttgctggcg ctgatcttca gggctgtgtc gctcgagttc
    13381 cgcggcaaga cggcgtcggc aacttggcga cgaatctggg atttcggttt ttttgcgggc
    13441 agcctgctgg ctgccttgct gttcggtgtg gccgtcggca acgcgatgat cggtatgccc
    13501 atcggcccgg atatggagtt tgtcggttct tttctcgatc tgctccggcc ctacccgctg
    13561 ctgatcggac tgttcaccgt ggcgatgttc gccatgcacg gggccctcta cctgtacctc
    13621 aagaccgagg gacggctgca ggaacgcatc gccagttgga tgtggcacac cttcggtgtc
    13681 ttcttcgtcc tgtacctcct gacgacgatc ttcaccctgg ccacagtgcc cgacgccatc
    13741 cgcaatttcg ccgctcaccc ctgggcctgg ggcgtggtgg tgctcaacgt cttggccatc
    13801 gccaatattc cacgggccat ttaccttcga cggccgggat atgccttcgt ctcgtcctgc
    13861 tgcacgatcg ccgctttcat tttcctgttc tcgattgcct tgagccccaa ccttgtgacc
    13921 tccagcatcg atccccggta cagcctgacg atctacaacg ccgcttcatc ggcccggacg
    13981 ctgaccatca tgctcatcat cgccgtcatc ggaatgccct tcgtcctggc ctataccgct
    14041 gtcatctact gggtcttccg cggcaaggtt cagcttggcc ggcacagcta ctgatggggc
    14101 gcgccccggc cgcatcactg cccaaaggag ggaaaaccag atgccttaca gcccgtgcgc
    14161 cttcatgaga ggcaacgtgg ccctttcaga accacgaccg tcagagagta ccgtcccgat
    14221 agacgatgaa caggacgcca ggcgagtcct gtgtgcccca tccaaggtgg gccgggggtc
    14281 atccctcctc cacgccgctc cgcacacacg ctcaccgccg gagcatgcca tcgtgttatg
    14341 tcgtcaccga aatcagcgga gatcctctgc ccgcacccgg cgggcacctg cggttccggg
    14401 ctctttgata tattgatacc ttacggccgg cgatctgtgc ggcttgcgcg cacgttgctg
    14461 aatcgcagtg cccccgggtt gcggcccgca gaaaggcggt tcgccttcgc agccctactc
    14521 tggcggactg ccgggctgga aacgacctcc ggtcttaccc cgtcccagcc gaacgaaccc
    14581 aaaagtcaag atagagaacc tccgcccaag cacggccggc ttccccgcca gacccgggca
    14641 gttgccggat cgccgcgcca aacgaaccca aaaaatgacc taaacgattg atagaaaagg
    14701 ggttgcgatc gtcccactgg cctgccatcc ccctcggaat cccttggctt gagcccccct
    14761 tttctgtccc ccggctggcg gggaagtcgg gactcctcgg aatggggcac ccggttggac
    14821 acgcggcggc cgccggcgag agtcgccgac caagagtctt ggagcgactt ggttgctgct
    14881 acaacgatgc cggagaagtc aggaacccct gataagcatc ttctacccga tgcctgcacc
    14941 gctttcacaa tggcgtaaag ccgtttaccc cctccggttg cataagaatc cccgcaggcg
    15001 cgcggagaag catgtccgaa tcgggcgtct tgtgccgaat caccgctgtc tccggagttc
    15061 accggtgacg aggtccatgg gcgacggcag aacacggccc attctcagtt ccatgttcac
    15121 aatcctgcat tcccctgcgt tgccgtgtgc aaccctcggc tttacactgc cgagcggcca
    15181 gggaggacga atcatgaaac gaccgatgcc aggtcttggc tggttcggaa gcacgctgct
    15241 ggtcagttcc attgcggtcg cggcaaatga tgttggcttc tatcagatcg gttcggcctc
    15301 ggtcgtcata acgccgaagg tggcccatga cgccccaccg gtgtggctgg ccgggtacgg
    15361 gcagggccga caggccgagg gcgtgcacga cgacatctac gcgcgggcca tattcatcca
    15421 tgacggccaa ttcggtctgg ccatcgttgc atgcgacctg atcggccttt tccacgacga
    15481 ggtggcgaag gtccgaaagg aacttggccg gctgcaactg agcccgtcga tcgattacgt
    15541 ccttgtcagc agtacgcaca cgcacgccgg gccggacacg ctgggactct ggggccccgt
    15601 tggccggacg ggcatcacgc ccggttacct cgatgaagtg cggacggcgt gcgtcgatgc
    15661 cgtccgccgg gcgcatcgga atgcccggcg cggcacactg cggatcggaa ccgccgatgt
    15721 gaatcgtctg gccgaactga tcggcgactc gcggctgccc aaagtgatcg actcgcaact
    15781 gaccgtcatc caggcgacga atgatggcgg cgagatgatc gttacgctgg tcaacatgcc
    15841 gtgccaccct gaggttctcg gttcaaagaa caagcagttg agcagcgatt tcccttcgac
    15901 gttgcgccag taccttgaaa cgaagttcgg cgggctggcc gtctacaaca gcggggcggt
    15961 cggcggcttg ctctcgcctc gcgagccgaa gtctgacccg ttcacaaatg agccgttgcc
    16021 ggaggatgaa atcggccgga tgatcgctta tggacggatc atcggacgta ttgccgaggg
    16081 cgccatcgac caagctcagc ctctgcgcgg gccgattcgt gctatctctc gggaagtgca
    16141 aattccgttg tggaacccgt tttacaggct gggcggatcc atcggggttc ttcagcggcc
    16201 gatgctcgac gcagacgggc gacctgtcaa gatgcagcag gccgccacgg cgaccgccgc
    16261 ggccggcgca ccggccgagg gcgatctgta tctcaagacc gagatcggcc tcatccagat
    16321 cggttcgctt cagatcgcgg cggttccggg cgaactctac ccggagctgg ccctgggcag
    16381 attccagcaa ccccaggaag ccgcggccga tttcccgaac gccccgctgg aaccggcgat
    16441 ctatccgctc atgaccgggc ggttcaaaat ggtcatcggg ctggcgaatg acgagatcgg
    16501 ctacatcatc cccaagtcgc agtgggactg gtcctcgcca tacgcctatg gccgcaagga
    16561 gcgccagtac ggtgagatca actcctgcgg gccggaagcc gccacgcgcc tgatggcggc
    16621 gtggcagtcg ctcgtcgcac agcggtagac gcggaatcgc gtgcgacgtc cgacctcggc
    16681 gattgggagg cggtcatggc ggcaaacgcg gcctgccaat ccggggtttc aaggttttga
    16741 agacatgagc atgtcggcaa cgcctgtcac cgtcgggagc agacgctttg cgatcttgcc
    16801 tgtaggaccc cgcggcagat cacgatggat aatgatctgg cgagggactt tgaagctggc
    16861 cagatgacga cggcagtatt cacgaagctc gatggcgctg acttgggcgc cgtccttggg
    16921 aacgacaaaa ccgaccacaa cttcaccacg cctggggtcc ggtacgccga tcacggcgga
    16981 ctgggacacg gcagggtgat cgtcgaggac attctcgatt tcccggggga acacgttctc
    17041 gccggctacg atgatcagtt ccttcttgcg gcccgtgatc atggtatagc cgtctgcgtc
    17101 ctgtcggcct aggtcgccgg tacggagcca gccgtccggc atcaggacgg ccgcagtctc
    17161 ttgcggcctg ttgaggtatc cctgcatgac attgggacct cggacacaga tctcgccctg
    17221 ctcgcccgac ggcagagcgc gtccttgctc gtccctgatg ctcacctcca cgtcgggaat
    17281 cggcagacca atggtcccgg ggcggtgagc ccatggctgg ttgacggaga ccaccggcga
    17341 tgtttcggtc agaccgtagc cttgaagaat ggtgaaatcg agccgtgtcc ggcaatcctt
    17401 gaaaacggcg tcgggcaagg cttcgccgcc gctgaccgca atcctcagcc ccctgagcat
    17461 ttccgacgag acgttcttca cgcgggcaag cgccgcgaac atgctggccg cggccatgaa
    17521 cacggagata cccccgcctg caagggcccg aagaacctcg accggctgaa agcgagtctg
    17581 gtactcgacg gtcgcgccga acaaagtggg caggatgagc gtcgcgagga ggccgaagct
    17641 atgggagaga ggcaatacgc caaggaaccg gtcatcgtcg cgaagcagca catgccgcac
    17701 ggtggcggac gcgttggagt ggaggttgtt gtgcgaaaga cagacccctc ggggcatcgc
    17761 ggttgttcct gaggtgtaca ggatcaccgc gagatcatcg ggtttgctct ccggcaccgc
    17821 cggaaatcgc ggggggccgg agggtgacgg cggtggttcc tgcggcaggc gatccaggaa
    17881 caccgcctgt gcaggcagcg cattgacgac tttctcgagc gcggagcagc tcaggatgag
    17941 atcgacgccc gcgtcttgaa tgattgtttt gatctccgag ggattgagca gaacgttgag
    18001 aggcacaacg acgcgaccgg cccacaagac ggcatagaag gcggcaacag cgtccgtcga
    18061 cgatggcaga aggatcccaa cgttggcccc acggcttgca gaggtgacca tccgagccag
    18121 cgccgccgct cgggagacga gttggttgta ggtcagttgg cgcgtctcat cgagaacagc
    18181 cgtcttaccg gcgcatcgat ccgcagtgtc gaggaagctg gtcagaagca tcgtgtcgcg
    18241 tgcaaccggc aggcccggtc gttcgcgttc ggccgcctcg accggacgcc gcctgcgtcc
    18301 cctttcgtgg tgcgataggc tcagaacgac tctattggcc cgtccgtgtt ggcgtcttgt
    18361 taatggaccc aagccgccgt gtcaagccag tgcgtccaag ccgcttcagt gcccccgggg
    18421 atctgcccca gtaatagcgg ccaggattcc acgcggcctg aatgggcccc agacgcttcg
    18481 ccgacgttct gggaagatga ccttggcctc ctcacggcat tctccaggcc gccctgccag
    18541 tgacaaaacg tcgtgttgtt cgacaaccga atcgaagctg gtatcatcga gcctcaggtc
    18601 agatcttgga tgtccgggta tcgacagggt tgacatcacg tcaccgctga tcgccgagac
    18661 cacaggccgt ggaacggagg caatgtgatg agagttcggg cccttctttt cgagaagccc
    18721 accggcgttt tcttgtgggt cgccgtccct gcaatgctct tcttcagtat cgcccccttg
    18781 ccggccgccc aacaaccgga tgctgaaccg cccgtgccca aaggtttcgg ctccacggag
    18841 gaagtcaagg aggcgatcgg gcagggcaaa gtcaggctgg tcaacctgtt catcccgctg
    18901 ccggagaacg ttgaggttat caacaatgtc gagtacggca gagtgggcga aagatcgctc
    18961 caactcgacc tttatcagcc caaaggccta gtaagccggt cccggcggtg atcctgatcc
    19021 acggcggagg atggaaaagc gggaaccgcc agatctatcg gtactattgc atcaagttgg
    19081 ccgagaaggg ttacgtcacg gccacgatct cctaccggct ttcggaggag gcaccatacc
    19141 cggcggctgt gcaggacgcc aagtgcgcgg ttcgctggct gcgggccaat gcccgcaaac
    19201 accacgtcga cccggataag atcgccgtgc tcgggggctc ggccggcggt cacctggcga
    19261 tgatggtcgg gtactcatcg ggggtgaaag ccctggaggg caacggcggt aacgcgggcg
    19321 tcagcagcgc cgtgcaggcg gcgatcgact tctatgggcc tgtcgatctg actgctgatg
    19381 aagccaagaa ggcaggggcg gtgatcgatt tcatgggcgg caagcgatat aacgaggccg
    19441 aggcgcagta ccgcgaggcg tctccgatca ctcacctggc aaagggcgcc ccgccgacgc
    19501 tcatcctgca cggcaccatc gacgacacgg ttcctatcgc gcaggcagac ctgttggcca
    19561 ggaagctcaa ggaactgggt attccatacg agtacgagag gttggagggc tggccccaca
    19621 ccatggattt gtccgaggat gtcaaccggc gttgtttgtg gcgggtcgag cgggttctgg
    19681 tcaagcatct tccggttcca cctggggccc tgccacccgc ttcaagcacg gcggcggagt
    19741 agcagcggtc aatgcggcaa gggagcacgt cccgtgaaac ggcgagattt tctcaaagca
    19801 accacgctgt cggcggcggg tgcagccagt gagaccctgt ggttggggca ggcaggtacg
    19861 gccatgagtc aaaccacccc gtcttcgaac cccgcggtgc tcccgcgacg tgactacggg
    19921 cgcaccggag tccaactgtc tatcatcggg ctggggggca tcgtgctggc gggattggag
    19981 cagcagcgag ccgaccgcat cgtggccgag gcggtggagc gcggattgaa ctactttgac
    20041 gtggccccca cctatggcga cgcggaattg aagctcggac cggcgctggc gccgtaccgc
    20101 aagcgggcgt tcctggcctg caagaccacc gagcggcggc gcgaaggcgc ggcgagcgag
    20161 ctgagacgtt ccttggaacg tcttcggacc gattacctcg acctgtaccg gctccacgct
    20221 ctgacggacg tacggaaaga cgtcgacgcc gcgttcgctg aaggcggggc gatggaggtg
    20281 ttcatcgaag cgaagaagag cggcagggtc cggcacatcg gtttctcggc tcactcggtc
    20341 gaggccgcac tggcggcgat ggagcgctac gaattcgact cgattctgta cccggtcaat
    20401 ttcgccacgg attacgcgaa cgacttctcg cggcaggtca tcgagacggc tcgaagaaag
    20461 ggcgtggccg tgctggcgct gaaggccatg gcccgccagg cgtggcaaga gcatgattcg
    20521 cggcgagccg cctacaacaa gtgctggtac gaacccctga ctgacccgcg cgaggccgag
    20581 ttgggcgtgc gatacacgct cagccggccg gtgaccgctg cggtgccgcc gggcgaggag
    20641 tcactcttcc gcctcgccat ggacgttgcc atgcgttttc aaccgattcg gccgagcgag
    20701 gccgaggaac tgaaaacctt gactgccacc ctcaagccga ttttccccaa ggtctgatcg
    20761 cgaggaggta ccatcatggc cgcattctcc agggttgcgt gcttcgtggt cctggcgacg
    20821 ttgctgccgg catgttcgcc ggccgcgcag gtgtgcgagg atgtcccctt tcgacaggtg
    20881 cgggcgattc atttcaagaa cgaggtggat ctgtcggatt atgaacttcg cacggtccgt
    20941 gtggaccgcg acgggaaagt gctgatcaac accaacaggg gtctgctcaa gggctacgat
    21001 ggtcgactgg tccgttaccg ggagctggcc ggtatatccg agctggatca ctacgatctg
    21061 gagctgctca agggcaaatt cgtcttcctg acggacaaga tgcttctgcc ccttcacggc
    21121 gcgggtgtcg attttcacca caatacggaa tcgcaatgga ctcggttggc cgccgcggct
    21181 tccggccact acctgcttct gtcaaaggcc aagattgctg agatcgcggg gggcaagaat
    21241 gcgcagatcg acaatcccgg cttcagcgaa gtgattctgt gccccttcga ccgtatgttc
    21301 gtcctgtacg cgaccgacaa gctggcacgg taccaaggcg gccggctcat ccccctgccc
    21361 gccccgcccg ccagaatcac tggcgtggtt ccagcctcca acggcgagtt gaaggtggcg
    21421 acggatcaag gcctcttcac cgtcacggac aagtcggtgg tcgccgatct ccggccgttg
    21481 ccctggcagg atctgacttg catcactcgg gatgccgacg ggcggctctg gatcggatca
    21541 acccgaggag ccttcagcct cgaccatgcg ggagagatca actactacgc cggccgccga
    21601 tggctccaag acgaccaagt gatcgacatt cacgtcgata tgcgaaacga cgtctatgtc
    21661 ctcaaccggc gagggctttc tcagcttcgc ttcgagatga tgaccctggc ggacaaagcc
    21721 gcaatcgaac tgcgcaacct gcgcctcaat cacatccggt atgggctggt ctccgatgcc
    21781 gagcttgtcg atgccgatta tgcgcatctg cggctgcacg atacggacaa cgacgggctg
    21841 tggtcgagcc tgtacctggc ctccgaggcc tttcgatttg ccgtcacggg ggccgaggac
    21901 gcccgtgaga acgcgattga cggattcgac gcgatcgagc gactcgtgac catcacctcc
    21961 attcccggtt ttcaggcacg gacttttgag ctcgaagggt acaagcgctc ggatccggag
    22021 cggtggcgga cccgaccgca acatgatttc gaatggaagg ggcacacgtc gagcgatgag
    22081 atcgtcggca cgttcttctt tcattcggta ttctatgaca cggtggccag ggatgaccca
    22141 gctctcagag agcgtgtggc gtcggtcgtg agggccatca cgagccacat tctcgaccac
    22201 aacctgtatc tggttgacgt cgacggcaag cccaccacct gggggcgctg gaaccccgaa
    22261 tatgtcaaca cgccggccgt cggcggcgac cggcgtctga actccatcga aatcctgtcg
    22321 tttctccagc ttgcttacca cgtcaccggc tcgcagcggt tcaaagatgc tttctacgac
    22381 ctcgtccgca agcacggtta tgcggacaat acgatcaatt acctgcccga ccccaggggc
    22441 gaatggaatc actcggacga cgaactctat tggctgagct actacaacct catcaagcat
    22501 tgctttgacg acaagttgaa ggcaacgttc ctgaagagcg ttcgcgccca caaggaggcc
    22561 acgcatcgca agaggaaccc ggtgtggaat ttcatcttcg gcggcgtgac gggcgaggcc
    22621 atcgacttgg agggaagcat ttttgtcctg cgaaggtttc cgctggataa ccggaattgg
    22681 cggatgcaga actcgcatcg cctggacatc cggatcgacg cccggccggg tgtggagccg
    22741 gaggccgctg ccgtcctgcc gcccgatgag cggcgcatcc agaaatggaa cggcaacgag
    22801 atgggcctgg acggcggcgg ggatgggaac agcccggagt ccggagcaga gtaccttctg
    22861 ccctactgga tgggaaggta cttcgggttc attactgctc ccaggtaggc tccagagtgc
    22921 ggcgggactc ggtcgctacg gcgacctcca ccctgcccca ccctctggtc ccgttgattc
    22981 tcgcgcacgc acggaaaccg cggcgccggc tgtccgttca aggtgtggga ccgctgacgg
    23041 ggagagccgg ttgggtgccc acaatccgga cgttcgttga aagatagatc atccggccct
    23101 tgacttcgta gacggcttcg ccgaagacgg ccgcaaagcc cttctccggc tgcttcaatc
    23161 ggtactcgta ttcctcgccc gcgggatcaa tggcttccgg ccgccacttg gcgtcccgga
    23221 aatccttggt gggcgattcc gcaacccagg ccgaaacgct ggagggcttg acgtccgacc
    23281 agatcgtcaa cagcaaggta tcgccgtcct ccttcatgtc ccaggtgagc ttgggaaaat
    23341 cgagtttgcc cgctgccttc agggcaaagg ccgacacgct gctgaccacc cgaaccatgt
    23401 cgttcaggcc gtggccgacg ttcgggacat aaaggatgta cttctcgccc tccagcccgt
    23461 cgtagtacaa gttgagcgcg tctaacgtcc aatagcggtc gttggtgccc aggagaatga
    23521 gcttggggat cttcagccgc ttgcggtacg agtatggatc gacaatagcc agcagcttct
    23581 gtcccgtctc g
//
LOCUS       DAOTPW010000065        23264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2265_length_23264_cov_5.727519, whole genome shotgun sequence.
ACCESSION   DAOTPW010000065 DAOTPW010000000
VERSION     DAOTPW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23264
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_23264_cov_5.727519"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..957)
                     /locus_tag="P5316_07820"
     CDS             complement(139..957)
                     /locus_tag="P5316_07820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRR84903.1"
                     /translation="MFRRSSITLLCAILLAVPVACRSGSRPVPTPTSPVRSGFAYDRG
                     GIIRGPRDCKQIALIFTGGKYGEGTAAILDALSARDIKGSFFVTGDFIRVPEHQAHLR
                     RVVAEGHYLGPHSDAHPLYCSWEDRDQTLVTESDFRADLEKNLADLSRHGRTREQMRY
                     FIPPYEWYNEQIVTWARDMGLVLFDFTPGTRSNADYMADSDPRFVSSPRIVESILEYE
                     SSKPDGLNGFLLLLHLGAGPDRTDKMHAHLGPLLDELARRGYRFVRVDEMLGGQ"
     gene            complement(1001..2686)
                     /locus_tag="P5316_07825"
     CDS             complement(1001..2686)
                     /locus_tag="P5316_07825"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRR84904.1"
                     /translation="MQELSKLCLWLTISCVTGGCSKMVEYDLLIRNGEIYDGSGRAPF
                     RGDIAINGDTIAEIGDLPAARGRTEIDARGLAVAPGFINMMSWANESLIADGRSQSEI
                     RQGVTLEVLGEGNSMGPLNDKMKADEKERQGDIQYDIEWSTLREYLDYLVRRGVSCNV
                     ASFVGAATVRIHEIGHENRRPTAQELERMKALVRQAMEEGAVGLSSALIYAPGCYADT
                     EELLELAKVAAEYDGLYASHLRSESEQLLEALDEFLSIARQAKIRAEVYHIKASGRRN
                     WHKLDAMLGRIEAARAEGLHVTADMYTYHASATGLDSIMPPWVQEGGHRAWVERLKDP
                     AIREKVKQEMRMTGNAYDNGLIAAGSADNIILIGFRSEALKPLTGMTLAEVAAQRGRS
                     PEDTAIDLIIEDDSRVGAAFFTMSEDNVRKEIRQPWISFCSDSASQAPEGVFLKRNPH
                     PRAYGSFARLLGKYVREEKLICLTEAIRRLTWLPAENMRLDRRGRLSPGYFADIVVFD
                     PATIIDRATFKQPHQYATGVRDVFVNGVQVLRNGEHTGAKPGRVVCGPGVRKG"
     gene            2790..4742
                     /locus_tag="P5316_07830"
     CDS             2790..4742
                     /locus_tag="P5316_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84905.1"
                     /translation="MSEVPDGTSRTAPTALPMTAYRSMTVIMATAAVVFVFWRAWVCD
                     DAFITFRHVANCLNGNGPVFNPGERVQGYTHPLWFLILLTGSTLLDPHPLAMILGLVS
                     TAAIILLLSRFFRARQHNGLCLMAAAAMLLSSPTFIEYQTSGLETCLTHLLVVWMWAW
                     LLTFTTGDREQTGNPALSSPPLTKGGQGGVPHQPQTRFAPSPAAIVLICSLLMLNRPD
                     HLVPCVPLFLLALHLLRGRRRRAWLLSLAALLPLLAWYGFATVYYGSPLPNTAYAKTG
                     MPLGTTILRGLAYLRNYSAAEPIHGLIIPIVLIIQTVIAVRDFRAKRPGGQVRLALVV
                     AVWLHVGYVVMVGGDFMRGRFLNLRLVATAILTGDLIGRLSPRRFWKGVFSLVLIAGI
                     AWSVGRAVWQFAAFRMIGPPGVYPLLAIILLAITALGGIAAVIFLIRRRPLAPGPVTS
                     ILVLTAAGLCTLFDFKPRTTEFAVDGIADEYAWYAGTWRESRFARPAQYPEECVNDRV
                     RLGQSANRYAGRYGPITIAWDTTGFLGYLAGPKVQIVDKLGLTDSFIARLPADPNSRI
                     GHLLHDIPKEYLRSRMVINELPDWRRRLDEGDPTLIADARTLPPTAPWADTSLEQRWK
                     EIQTITKGCLFSGERWKLITGYVRGR"
     gene            4944..6011
                     /gene="pheS"
                     /locus_tag="P5316_07835"
     CDS             4944..6011
                     /gene="pheS"
                     /locus_tag="P5316_07835"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRR84906.1"
                     /translation="MLEQLTEQLKKAAAEAERLLAESKTVDALRQAESRLMSKQGPLG
                     SLLGQIGKLPAEVRGTAGKEINQAKARITEMFAAAKAALEQAADRQASAEQAFDPTLP
                     GPVVPRGSVHPVTAVQWEVEEIMARIGFVVVGGPELETDYYNFEALNIPAMHPARDMQ
                     DTFWLTNGWLLRTHTSPCQVRAMLRLGPPLSIIAPGRVFRYETEDASHANTFHQLEGL
                     MIDRHISIANLIAVMKLILTEIFHRDVKVRLRPGYFPFVEPGFELDMQCGICGGSGCP
                     TCKRSGWIEILPCGMVHPNVLRSGNIDPREYTGFAFGLGLTRLAMMKYGVADIRGLNS
                     GDLRAIIRPEQRNGAAIIDKD"
     gene            6033..8255
                     /locus_tag="P5316_07840"
     CDS             6033..8255
                     /locus_tag="P5316_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84907.1"
                     /translation="MTPSAPVDFPAGRQAIPAPDIPTEDIRRSTYRLTAAGLVCAAAL
                     FVLYRTWVSDDAFITFRHVSNFLAGHGPVFNPGERVQGFSHPLWFFLLAAGGLLFDVY
                     GVAVALGILLTVAMLGTQAWLLRNERHGVLRLQFMALMLLSSKTFVEFQTSGLETSLT
                     NALAGSLWALLAARGLAGKPPPLTAAVWLCGLLVLTRPDLVVLTTPIALYLLACLLRK
                     PPELATPANQQNRGNSAGGQSRRWLGPAFALLAVLGWYGFATVYYGTPLPNTAYAKVT
                     MPFSAALPMGAHYVMDYARSEPIHALLILTAIVAAPTLAVSNRLRGHGGTGVLGWLGL
                     GIACHVAYVISIGGDFMRGRFMDPALTASVVLTTWLLGCLLSRTRVSPKAVSAAIGLV
                     GAVFLLIQVRSPVALSCIGRGADELVYRITRWPATTILGMIGYATCCVLLVGWIGRTH
                     GSRAGSAFTVLVAIEVLVLLGLVGFWQPTWGGIGLLAGAGLCAASCLAIGFAGGGLRH
                     KGAGPAACLLIAAGASLVDVGRPREGGQGAISDDYSFYWVGKWHNPFRHPTEALRGST
                     GSWLRFGMAACRYARLYGPITVATDSLGIVSYYAGPDVRIIDLYGLTDPYLARSDAPP
                     CGRPGHIKFRIPPGYLEDRSTVNLLPNWLERLNLMDPRLANDARRLPAEAMWDDADKE
                     RWLATQRVISSPLFSRQRLADIPRYMWPERHYVPDSPDGDPVRARVSPRLGAIPTRGP
                     "
     gene            8406..10814
                     /gene="pheT"
                     /locus_tag="P5316_07845"
     CDS             8406..10814
                     /gene="pheT"
                     /locus_tag="P5316_07845"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRR84908.1"
                     /translation="MLISLNWLRDFVDVPQDLDPGDLAERFTCTTAEVEGVQHITCSA
                     SGLIAAGIVNVEAIPGDKPLFLVKVDLGNKQVDTVTIAEGLKSGDRVVFAPPGSTLPG
                     VGQVAERKTAGRTSAGMIVPGDALSLPTVGQRAMWLPPSTRPGSPVDMSLFDDWVIEV
                     DNKSITHRPDLWGHYGIAREVAAICDRRLKPYPIVPPQELDDPALPAIPIVIDDPSKC
                     PRYSALRFTGVRPQPAPLWMQVRLAHVGLRPIDILVDLTNYIMAELGQPMHAFDCANI
                     DRIEVALAKPGEKFVTLDGFTRTMPEGALMIQCNRQSVALAGIMGGANTEVTAKTTDL
                     LLESANFEAATIRRCATALGHRTDASARFEKSQDPHNTVIGIGRFVYLARPELPDMKF
                     TSRLSDCFPNPPKPITIRVDPAFVGRYIGRQVPADRIMSILAKIGFAVKPVDGMLEVG
                     VPTWRATKDVSIEADIIEEVARFIGYGTITPALPEVTVRYAEPEAITRLERQTLTLMC
                     GGMSYAEVHRYIWFDAEWLKLLGFDPGPTLSLKNPVAAGTERLRTTLLPGLLAAVDLN
                     RRHFERFELLEIGSALPYGEKEAPEKTEQRHLALAVVAPGRKPAHEDALILRLKSDLE
                     TFAQQVLKGHFQFAAEKAAAPWEHPAKTAAVKLDGLLVGRITAVPAALRRRIDEHLVA
                     WSIALAEINLSLLAERRPEPRKLAPVPVYPRINLDFSVLARSSRRYEDIEGALRTYDH
                     PLLRRLAFVDSYEGGSVPQGMRSFTFRATIGDPSRTLTEQDIQDFRSTFLEWMKSQDL
                     ALRT"
     gene            10818..12392
                     /locus_tag="P5316_07850"
     CDS             10818..12392
                     /locus_tag="P5316_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84909.1"
                     /translation="MKQPVQRPVSGAPAVLSCVAALVLMPRPAAAQMLPPSTIDADTV
                     WSGVIEIKGEVCIRDAAVRVEPGSTIRFVGGPGSNGASIRFGGSLSGRRFSSRSRLTL
                     AGTAERPITVETPAGHPPGFISGDLESGCALVARHVVFRGLGSAVSGKRGTPAVFLIL
                     NSPDSDLWLSHCRFTDCGRIHVEFIGGAATAEIESCTFVRTIGDTTIEFLGTGEGLRV
                     ISDNISSAAFRIECPQTLLKNNVLVGEWAAISVNARNVSGIAITGNYVNCATDRDEGR
                     YALRCSAEDAVVTRNVLLGGTYVIEQSPRTVTGNVLVGAAALQPGVSPGGAPATKLTT
                     THYLLSKPAPQARIADNLFLGPAYAAISAGRESPQIRIEHNFFDGWGVAKRAVHFNLL
                     MPASREAPLAAGITGNIFARYRAAPVADDAKHVGTAAQAGLNVFAEVPDTLYELSGIQ
                     KAAPGDLVLPALADLRLQAPVATRSALSAEAQLLQRQLTVAKVREMWFDAYRPVQGSP
                     LIGSRPAGPRPDSSPE"
     gene            12487..13596
                     /locus_tag="P5316_07855"
     CDS             12487..13596
                     /locus_tag="P5316_07855"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84910.1"
                     /translation="MTHGLPENAAFSRRRLLVGGCAAVVGPATVRGSVQQAATRHAQG
                     PDTSSWPKPIKAFCIDFNWAGNQPSPPGTYAHADPADHVRWYRDLGANTIQTFCVTYN
                     GYAWYPSDVAPVTPGLKSPNFLGDMVELGHKAGMRVLGYFTLGANPYWEKLHPNLVHG
                     GDSDSIKIPMTLEYLDYFCRSVEDAILKTGVDGFMIDWVRPTQHKNWLDCEKEMYRQL
                     LGEEFPESGRPSTKMILEFDRRAMERAWRHIRWTVRATGPSVIWTNHPFLKSEYPIWT
                     GHRVLEEVDWVLNEAPELDHLDWLRRQVGPRTLILQNLCGWKDHDALMWKKIAPMALG
                     FYGFAKADEKTTLPDDTIEVNVKNIEILREAYHSL"
     gene            13932..14237
                     /gene="gatC"
                     /locus_tag="P5316_07860"
     CDS             13932..14237
                     /gene="gatC"
                     /locus_tag="P5316_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRR84911.1"
                     /translation="MTRKIDEAQVRHISHLSRLNPTEDEVRLFSEQLSAILEYVEQLN
                     EVDTTDVPPTAHALPVKNVFRADEPRTGLTPDQALANAPHREGNFFAVPKVLDQDSA"
     gene            14274..15743
                     /gene="gatA"
                     /locus_tag="P5316_07865"
     CDS             14274..15743
                     /gene="gatA"
                     /locus_tag="P5316_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRR84912.1"
                     /translation="MTAPTLLETRDSIRNRKGTSQACVRHMLDAIRELDPKIRAVISL
                     DVERALHLARQADERISRGQIDGPLAGVPLLIKDNLCTAFGRTTCASRILENFTAPYN
                     AHVVEKLEAAGAIIVGKTNLDEFAMGSSTENSAFTVTRNPWNLDYVPGGSSGGSAAAV
                     AAGITAGSLGSDTGGSIRQPASFCGVTGLKPTYGRVSRYGLVAYGSSLDQIGPFGRTV
                     RDVALLLGVIAGKDHRDSTSVDQSVPDYLAALEQPLASVRIGYAEEYFGEGLDPEVRS
                     AVEAAIELYRSAGATVQTVHLPHMKYAIACYYLIATAEASSNLARYDGVHYGHRTASP
                     RDYIDMYCSSRQEGFGAEVRRRIMLGTYALSSGYYDAYYLKALKVRTLIQRDFEQALR
                     QVDVIASPTSPTPAVRIGEKINDPLAMYLMDIYTVSANLAGIPAISIPCGFSRSGLPI
                     GLQLMGGHFAEEKLLRIAHQYQLRTDWHTRMPPIANGGS"
     gene            15759..17045
                     /locus_tag="P5316_07870"
     CDS             15759..17045
                     /locus_tag="P5316_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR84913.1"
                     /translation="MTSSVYHARWILPVASPPIENGRLVVQDGRIVAVEKAGTLDPAA
                     IDFGDSVLLPGFVNAHTHLMLTGCRGLRYHGSFTGWIRDLVTVHPRLSPPGTVRQSVR
                     EGLQQSLTAGVTTVADIGDGMEAIEAWAARRINIAGFLEVLGIGPKRAGKHPSAVQVA
                     ADMLRNGSLPLCAEAQKKGSLTQESLFVPGLSPHAPYSTDADVYREAIECVSGLSGIC
                     RGLCTHLAETPEEEQFLRDGTGPFRDLLEQFGLWDGSYQPPGCSPVEFMEHVAFLSHR
                     PLLVHCNYVSDSDLDLIAASEASVAYCPRSHAFFNHEPHRWREMLARGINVCVGTDSL
                     ASNHTLSVLDELRFLYRIDPSIPGEQLIHMGTLAGAKALGLADAIGSLETGKRADLAV
                     VTLDRPGSRDPVDAILTGTAQPSAVFVCGRNVQGMR"
     gene            17173..18117
                     /gene="cysK"
                     /locus_tag="P5316_07875"
     CDS             17173..18117
                     /gene="cysK"
                     /locus_tag="P5316_07875"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRR84914.1"
                     /translation="MARLYDNITQTIGNTPLVRIRRIIQAPATVYAKLEFFNPLSSVK
                     DRIGVAMIEAAEQAGRINKNTVLVEPTSGNTGIALAFVCAAKGYRLILTMPESMSIER
                     RNLLRALGAELVLTPATQGMKGAIAKAEEIAAQTPNAFIPQQFQNPANPEIHRKTTAE
                     EIWRDTDGAVDILVSGVGTGGTITGVAGVIKQRKPSFKAIAVEPADSPVITQRRSGEE
                     LKPGPHKIQGLGAGLILGVLDLSLIDDVVRVANEDAFAWARKAARQEGILCGISSGAA
                     LCAANEVANRPENAGKMIVVILPSCGERYLSTPLFHEG"
     gene            complement(18294..18914)
                     /locus_tag="P5316_07880"
     CDS             complement(18294..18914)
                     /locus_tag="P5316_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HRR84915.1"
                     /translation="MATRQTKKQNPKTSSGSGLYTLEVFLIGGPVTEEFVKENPVVSR
                     TIQIRGDQTLDKLHFAIFDAFDRDDEHMYEFQIGGKGPQDPGARCYVLPDARDAYDRR
                     QAGDLTRTRIGTLGLKVGDAFGYWFDYGDDWWHQINVISIEENIPPGEYPRVTQRVGE
                     SPPQYPDPEDEDEEWDEDEDDESDEDEDDDGEWEDPDEEDDADEED"
     gene            19204..19752
                     /locus_tag="P5316_07885"
     CDS             19204..19752
                     /locus_tag="P5316_07885"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRR84916.1"
                     /translation="MRYVSRKLGPLLIAIVVGLLPAGCASTETVGPAASLEARTDLIP
                     VGQKAPDFDAADQSGKRIRLAELLKKSSVVLIFYPGDFTPGCTRQLCAVRDDWSQFEK
                     HGITVLGVNPADAVQHARFVGENRFPFSLIVDEGSSITAAYGARGPDRTQRTVYAIRK
                     DGKVALAERGFVAHEKIFAALK"
     gene            complement(19926..20687)
                     /locus_tag="P5316_07890"
     CDS             complement(19926..20687)
                     /locus_tag="P5316_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84917.1"
                     /translation="MSRRHRHVSSRRLRRRLRSQRPPSPSGLRLIATGGHKGEPVVLE
                     MLSFEITEDPIPDPTLDRLRPEDREMILEVGSRVMEGATDQVPILERLAREFPQVPKV
                     YNYLTIAYSNAGLYAKAEEMAEEAYSRFPNYLFAMINYAHALLRRWHIRRVEQILDNR
                     FSIHLWITDRKRYHVSEYVAFNGLMARYFSAIGEDEIAERYYKMLLEVAPDHPGTKTI
                     DRVIHPTPMGSLLKRLTRWAHAKEADQEPVRAGGK"
     gene            complement(20796..21836)
                     /gene="istA"
                     /locus_tag="P5316_07895"
     CDS             complement(20796..21836)
                     /gene="istA"
                     /locus_tag="P5316_07895"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRR84918.1"
                     /translation="MLPTVEVFSRLRSLGYTGGKSAVYELVRSVRPRKNQGPEVRFEG
                     VPGEFSQHDFGSVNVTYADGTSEKIHFFASRLKYSRWTHVVIVPDERVESLIRALLKA
                     FESFGGVPLRAVFDNPKTIVLSRAQGFINWNPVFVHVPVDYSFGVELCRPRRANQKGS
                     VENLVGWVKGSFFKVRRFHDRADLEQQLIAWLEEVNLQRPSRATNQIPAERMKAERER
                     LTPLATPADDYPLRVAVTVGTTGFVEYERIRYSMPPTAIGIPGTLFLYPDRVRIVTRS
                     GIEVEHPRRPAVGNTSYCSEHRVARLAAVHGQRAKLYQKRQEILELGPPAEGLLTCGG
                     LASSRSVFGVSE"
     gene            complement(21870..22070)
                     /locus_tag="P5316_07900"
     CDS             complement(21870..22070)
                     /locus_tag="P5316_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84919.1"
                     /translation="MISMLDRVKIWALRQAGHTLEEIAAAVKVGKRSVQRILKEPPIT
                     SLESAPTPGSRQIGRPSRVEAF"
     gene            22579..22652
                     /locus_tag="P5316_07905"
     tRNA            22579..22652
                     /locus_tag="P5316_07905"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22613..22615,aa:His,seq:gtg)
     gene            complement(22939..>23264)
                     /locus_tag="P5316_07910"
     rRNA            complement(22939..>23264)
                     /locus_tag="P5316_07910"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         4175 a         7551 c         7021 g         4517 t
ORIGIN      
        1 ccagcggtca cggtagggca gcgtcgaggt cgccacagcg accgagacct gccgtcttgt
       61 tgagccggag ccggccccag cttccggggg cgagcagatc ggcaggtctc cgctgccttc
      121 gacggccccg accatgccct actgtccgcc gagcatctca tccacgcgga cgaagcgata
      181 gccgcgtcga gcgagctcgt cgagcagcgg gccgaggtgg gcgtgcatct tatcggtgcg
      241 atcgggacct gcgcccagat gcaagaggag aaggaacccg ttgagaccat ccggcttgga
      301 cgattcatat tcgaggatgc tctcgacaat ccgcggcgag gaaacgaatc tcggatcgct
      361 gtcggccatg tagtcggcat tggaccgcgt gcctggcgtg aagtcgaaaa gcaccaggcc
      421 catgtcacgc gcccacgtga cgatttgctc gttgtaccac tcatatggcg ggatgaagta
      481 ccgcatctgc tcgcgggtac ggccatggcg agaaaggtcg gcgaggttct tctccagatc
      541 ggcgcgaaag tccgactcag tcaccagggt ctgatcgcga tcctcccagg agcaatagag
      601 cggatgggcg tcggaatgcg gaccaagata gtggccttcg gcaacaaccc gccgaagatg
      661 tgcctgatgc tcgggaacac gaatgaagtc gccggtcacg aagaaggagc ccttgatgtc
      721 ccgtgcgctg agcgcgtcga ggatggcggc cgtgccctcg ccgtatttgc cgccggtgaa
      781 tatcaaggcg atctgtttgc agtcgcgagg tccgcgaatg atgccgccgc gatcataggc
      841 gaacccgctg cggacagggc ttgtcggcgt cgggaccggc cgacttccag aacgacacgc
      901 gacaggaacc gcgagcagga tcgcacacag cagggtaatg cttgaccgtc ggaacatgga
      961 tgtcctgctc gtcaaacgag cttcctcgcc ggcgaaacgc tcagcctttt cgcacccccg
     1021 gaccgcacac gacacggccc ggtttggccc ccgtgtgttc gccgttcctc agcacctgca
     1081 cgccgttgac gaagacatcc cgcacgccgg tggcgtactg atgcggttgc ttgaaagtgg
     1141 cgcgatcgat gatcgtcgcc gggtcgaaga ccacgatatc cgcgaaataa cccggcgata
     1201 accggccgcg ccggtccagt cgcatgttct cggccggcaa ccaggtcaga cgccggatcg
     1261 cctccgtaag gcagatcagc ttctcttcgc ggacgtactt gcccagcaat cgggcgaacg
     1321 atccgtaagc ccgcggatgt ggattcctct taaggaagac gccctcgggg gcctgcgaag
     1381 cggaatcgga acagaagctg atccacggct gacggatctc cttgcgaacg ttgtcctcgg
     1441 acatggtaaa gaacgccgcg cccacccggc tgtcgtcctc gatgatcagg tcgattgccg
     1501 tatcttcagg cgaccggcct cgctgagcgg ccacctccgc cagggtcatc ccggtcagcg
     1561 gcttgagggc ctcgctgcga aaaccgatca gaatgatgtt gtcggccgac cccgcggcga
     1621 tcagcccgtt gtcgtaggcg tttccggtca tccgcatttc ctgcttcacc ttctcgcgga
     1681 tggcggggtc cttcagacgc tcgacccagg cccggtgacc gccttcctga acccacgggg
     1741 gcatgatcga atccagaccg gtggcgctgg catggtacgt gtacatgtcc gcagtgacgt
     1801 gcaaaccctc ggctcgggcg gcctcgatgc gcccgagcat cgcgtccagc ttgtgccagt
     1861 tgcggcggcc ggaggccttg atgtgataaa cctccgcccg gatcttcgcc tgccgcgcga
     1921 tgctcaggaa ctcatccagc gcctccagca actgctcgct ctcactgcgc aggtgcgagg
     1981 cgtacaggcc gtcatactcc gccgcgacct tcgccagctc cagcagttcc tcggtatccg
     2041 cgtagcagcc gggcgcgtag atgagcgccg atgacagccc cacggcccct tcttccatcg
     2101 cctggcggac caaggctttc attcgctcga gttcctgcgc ggtcggcctg cggttctcgt
     2161 gaccaatctc gtggatgcga accgtcgccg ctccgacaaa cgaggccacg ttgcaggata
     2221 cccctcggcg aaccaggtaa tcgaggtatt ccctcagagt gctccactcg atgtcgtatt
     2281 gaatgtcgcc ctggcgctcc ttttcatcgg ccttcatctt gtcgttaaga ggccccatcg
     2341 agttgccttc gccaagcacc tcgagagtca cgccctggcg aatctcgctt tgcgaccggc
     2401 cgtcggcgat cagtgattca ttggcccagc tcatcatgtt gatgaagccg ggcgcgacgg
     2461 ccagtccccg ggcgtcaatc tccgtccgac cgcgagccgc aggcaggtcc ccgatttcgg
     2521 cgatcgtatc gccgttgatc gcgatgtcgc cgcgaaaggg cgctcttccg gaaccgtcgt
     2581 atatctcgcc gttgcggata agcagatcgt actcgaccat cttggaacag ccccccgtga
     2641 cacacgaaat ggtcagccag agacagagct tcgaaagttc ttgcatttca ccatcctact
     2701 ccgcatcccg tgcgcatgtc caaggctccg cctcctggcg taaccggccg atctggaatc
     2761 ctggtccgcc gtcggctacc gtgtcgggca tgagtgaggt acccgatgga acatccagga
     2821 ccgcgcccac cgcgttgccg atgaccgcct atcgatcgat gaccgtcatc atggcgacgg
     2881 cagcggttgt gttcgtcttc tggcgagcct gggtttgtga cgacgcattc atcactttcc
     2941 gccacgtggc gaactgcctt aacggaaacg ggccggtgtt caatcccggc gagcgagtcc
     3001 agggctatac gcatccgctg tggttcctga tacttctgac cgggagcacg ttgctcgatc
     3061 cccacccgct ggcgatgatt ctcggccttg tttcgactgc cgcgatcatc ctcctgctga
     3121 gccgcttctt ccgcgcacgg caacacaacg gcttgtgcct tatggccgcg gccgcgatgc
     3181 tgctctcttc ccccacgttc atcgagtacc agacttcagg cctggagacc tgcctgacgc
     3241 acctgctggt tgtgtggatg tgggcctggc ttctcacctt cacaaccggg gatcgcgagc
     3301 agactggcaa tcccgccctg tcttctcccc cccttaccaa gggggggcag gggggggtgc
     3361 cgcaccagcc tcaaacacgt ttcgcaccgt cccccgccgc tatcgtgctc atctgctcgc
     3421 tcctgatgct caatcgaccg gaccacctgg tcccctgtgt gccgttgttc ctgctggcac
     3481 ttcacttgct tcgaggacgc cgtcgccgcg cctggctgct gagcttggct gcgttgctgc
     3541 cgctgttggc ttggtacggc tttgccacag tctactacgg gtcaccattg cccaacaccg
     3601 cttacgccaa gacgggaatg ccgctcggca cgacgatcct tcgcggtctg gcttatctgc
     3661 gcaactacag tgccgccgag ccgatccacg gactgattat tccgatcgtg ctcatcatcc
     3721 agaccgtcat agcagtgcgt gacttcagag ccaagcgtcc gggcggccag gtgcggctgg
     3781 ccctcgtcgt ggccgtgtgg ctgcacgtcg gctatgtggt catggtcggc ggcgacttca
     3841 tgcgcggccg gtttctgaac cttcggttgg tggccacggc gatactgacc ggcgatctga
     3901 tcggacgctt gtcgccgcgg cgtttctgga aaggcgtctt ttcgctggta ttgatcgccg
     3961 gcatcgcttg gagcgtgggc cgggcagtgt ggcaattcgc ggcgtttcgc atgatcggac
     4021 cgccgggggt atatccgctt cttgccatca tcctcctggc aatcacggcg cttggcggca
     4081 tcgccgccgt catattcctg ataaggcgca ggccgctggc cccgggaccc gtgacatcga
     4141 ttctggtgct gacagcggcg gggctgtgta cgctttttga tttcaaaccg cgcacaaccg
     4201 aatttgccgt tgacggcatc gccgacgagt acgcatggta tgccggcacc tggcgcgaga
     4261 gccgatttgc ccggccggcc caataccccg aagagtgcgt gaacgaccga gttcgactcg
     4321 gccagtccgc gaaccggtat gccggccggt acgggccgat caccatcgcc tgggacacga
     4381 cggggttcct gggctatctt gccggtccga aggtgcagat cgtcgataag ctcggcctga
     4441 ccgactcgtt catcgcccgt ctgcccgccg atccgaactc ccgcatcggc cacttgcttc
     4501 acgacattcc gaaggagtac ctgcggtcgc ggatggtgat caatgagctt ccggattggc
     4561 gccggcggct cgatgaaggc gacccgaccc tgatcgcaga cgcccgaact cttccgccga
     4621 ccgccccctg ggccgataca agcctcgaac agcgctggaa ggaaatccag acaatcacga
     4681 agggttgcct gttttccgga gaacgatgga agctgatcac aggttacgtg aggggccgat
     4741 aggagcctct caaaccgttg acatcacttg ttccgtcgcg gctcgcgcga tccccatgtg
     4801 cgactggcgg gagtcgaaga tccttcatgc cgggcgcctg aggctgaatg ccgccggcgc
     4861 tccaaccgtt gccccgcctt gcattccccc cgattgcgtc ctatcgtacc ggctttacgt
     4921 cagtggttcg agaatgatag accatgcttg agcagcttac tgaacagttg aagaaggcgg
     4981 cggcggaagc ggagcgtttg ctggccgagt cgaagactgt ggacgccctt cgccaggcgg
     5041 agagccggct gatgagcaag cagggaccat tgggcagcct gctcggccag atcggcaagc
     5101 tcccggccga ggttcggggt acggccggca aggagatcaa tcaggccaag gctcgcatca
     5161 ccgagatgtt tgccgcggcc aaggccgctc ttgaacaggc ggccgacagg caggcatcgg
     5221 ccgagcaggc ttttgacccc accttgcccg gcccggtggt tccccgaggc agcgtccacc
     5281 ccgtgaccgc cgtccagtgg gaggttgagg aaatcatggc ccggatcggg ttcgtggtcg
     5341 tcggcggccc ggaactggag accgactact acaacttcga ggccctcaac atcccggcga
     5401 tgcaccccgc tcgtgacatg caggacacct tctggctgac caacggctgg ctgctgcgga
     5461 cccatacctc cccctgccag gtccgggcca tgttgcggct cggtccgccg ctgagcatca
     5521 tcgcccccgg gcgggtgttt cgctacgaga cggaggacgc atcgcacgcc aatacctttc
     5581 atcagcttga agggctcatg atcgaccggc acatctccat cgccaacctg atcgcggtga
     5641 tgaagttgat actgaccgag atcttccacc gcgacgtcaa agtccgcctt cgaccaggct
     5701 atttcccgtt cgtcgagccg ggtttcgaac tggacatgca gtgcggcatt tgcggcggaa
     5761 gcggttgccc gacctgcaag cgcagcggct ggatcgagat cctgccctgc ggcatggtac
     5821 atcctaacgt gctccgcagc ggcaatatcg acccacgcga atacaccggt tttgcgttcg
     5881 gtctgggcct gacgcggctg gcgatgatga aatacggagt ggccgatatc cgcgggctca
     5941 acagcggcga cctgcgggcg atcatccggc cggagcagag gaacggcgcc gcaatcatcg
     6001 acaaagactg agccacccat gggagcggcc acatgacacc gtcggcaccc gtggacttcc
     6061 cggccggccg ccaggctatc ccagcccccg acatcccgac tgaagacatc cgccgctcca
     6121 cataccgatt gaccgcggcc ggcctcgtgt gtgcggccgc cctctttgtt ctataccgca
     6181 catgggtctc ggacgacgcc ttcatcacct tcaggcacgt ctcaaacttc ctggcgggtc
     6241 acggcccggt cttcaatccc ggcgagaggg tgcaggggtt cagccatccg ctctggttct
     6301 ttctgctcgc ggccggcggc ctgctgttcg acgtctacgg ggtcgccgtt gcgctgggta
     6361 tcttgctgac cgtcgcaatg ctcggtacac aggcatggtt gcttcgcaac gagcggcacg
     6421 gggtcctccg actgcagttc atggccctca tgctattgag ctcgaagacc tttgtggagt
     6481 ttcaaacttc cggactggag acatcgctga ccaacgccct tgctggctcg ctctgggctt
     6541 tgctggccgc acgtgggctt gcgggaaagc cgccgccgct gaccgccgcg gtgtggctct
     6601 gtggcctgct ggttctgaca cggccggacc tggtggtcct gacgaccccg attgcgttat
     6661 atctcctcgc ctgtctgtta cggaagccgc cggaacttgc cactcccgcg aatcagcaaa
     6721 accgggggaa ttccgccgga gggcaatccc ggcggtggct cgggccggct ttcgcgctgt
     6781 tggccgttct gggctggtat ggctttgcca ccgtctacta cggcacgccg ttgccgaaca
     6841 ccgcgtacgc aaaagtgacc atgcctttct ccgcggcctt gccgatggga gcccactatg
     6901 tgatggacta cgcgcggtca gaaccgatcc atgcgctgtt gattctgacc gccattgtgg
     6961 cagcgccgac tttggccgtg tcgaaccggc tgcgaggtca cggcgggacc ggcgtcctgg
     7021 gatggctggg tctgggaatc gcgtgccacg tcgcttacgt catcagtatc ggcggcgatt
     7081 tcatgcgcgg tcggttcatg gatccggcac tgactgcatc tgtggtgttg acgacttggc
     7141 tcctcggctg ccttctatct cgcacccgcg tttcgcccaa ggcggtcagt gcggcgatcg
     7201 gtctggttgg ggccgtattt ctgctgatcc aggttcgttc gccggtcgct ttgtcctgca
     7261 tcggtcgagg tgcggatgag ctggtctaca ggatcacgag gtggcccgca acaacaatcc
     7321 ttggaatgat cggctacgcc acgtgctgcg tcctcctggt agggtggatt ggtcgcacac
     7381 acggctcacg tgccggatcc gcgttcacag tcttggttgc gattgaagtc cttgtgctgc
     7441 tgggactggt ggggttttgg cagccgacct ggggcgggat cggcctgctt gccggtgcgg
     7501 gattgtgcgc ggcgagttgt ctcgcgatcg gctttgcggg cggcggactg cggcacaagg
     7561 gcgctggccc ggccgcctgt ttgctcatcg ccgccggcgc gtcactggtc gacgtgggcc
     7621 gtccaagaga aggcggccag ggtgcgatct cagacgacta ctcgttctac tgggtcggga
     7681 aatggcataa tccgttccgc catcccacgg aggccttgcg aggctccacc ggttcgtggc
     7741 tgcgtttcgg catggccgct tgtcgctacg cccggctata cggtccgatc acggtcgcca
     7801 ccgattcgct gggcatcgtg tcctactacg ccggccccga cgttcggatc atcgatctgt
     7861 atggcttgac cgacccatat ctggcccgga gcgatgcgcc gccctgcggt cggcccggcc
     7921 atatcaagtt ccgtattccc cctggttacc tggaagaccg cagtacggtc aacctcctgc
     7981 ccaattggct cgaacggctc aacctgatgg atcccaggct cgcgaacgac gcgcgaagac
     8041 tccctgcaga agcgatgtgg gacgacgcgg acaaagaaag atggttggca acgcaacggg
     8101 tcatctcaag tcctctgttc tcaaggcaac ggctggcaga tatcccgcga tacatgtggc
     8161 ctgagcggca ctacgttccc gactcaccgg acggcgaccc cgttcgcgcc cgcgtctcgc
     8221 cgcggctggg ggccataccc acgcgcggcc cctgacaagg tcacaccact catgccggtt
     8281 gccgaagacc gcgtggcggc ccggcggcgg tcggatcccg atcgtccgcg aatcttgaaa
     8341 gatcctgctt gcttccgtat cgtactgtcc cgtaaattcg actcctgact gaaaaggacg
     8401 aacccttgct gatctcactt aactggttgc gcgatttcgt ggatgttccc caggaccttg
     8461 atcccgggga tctggcagag cgattcacct gtacgacagc cgaagtcgaa ggcgtccaac
     8521 acatcacctg ctcggcctcg ggtctgatcg cggccggcat tgtcaatgtc gaagccatcc
     8581 ccggcgacaa gccgttgttt ctcgtcaagg tcgaccttgg caacaagcag gtggataccg
     8641 ttaccatcgc cgagggcctg aagagcggtg accgcgtggt ctttgctccg ccgggatcca
     8701 cgctgcccgg cgttgggcag gtcgctgagc gcaagaccgc gggccgcacc agcgcgggaa
     8761 tgatcgtccc cggtgatgcc ctcagcctgc ccactgtcgg ccagcgggcc atgtggctgc
     8821 cgccctccac aagaccgggc agtcccgtcg atatgagcct gttcgatgac tgggtcatcg
     8881 aggtcgacaa taagtccatc acccatcggc cggatctgtg gggccactac ggaatcgccc
     8941 gcgaagtggc ggccatctgc gaccgccgac tgaaaccgta cccgatcgtg ccgccgcagg
     9001 aactggacga cccggcgcta ccggctattc cgatcgtcat cgacgacccg agcaaatgcc
     9061 cgcgctacag cgcgctgcgt tttaccggtg tgcgcccgca acccgccccg ctgtggatgc
     9121 aggttcggct cgctcacgtg gggttgcgac cgatcgacat tcttgtcgac ctgaccaatt
     9181 acatcatggc cgaactgggt cagcccatgc acgcattcga ctgcgcgaac atcgatcgaa
     9241 tcgaagtggc cctggccaaa ccgggcgaga agttcgtcac cctcgacggt ttcacccgaa
     9301 ccatgcctga gggtgccctc atgatccagt gcaaccgcca atcggtggcc ttggctggca
     9361 tcatgggcgg cgccaatacg gaggtcaccg ccaagaccac cgatctgctc cttgagagcg
     9421 caaactttga ggcggcaacc atccgacggt gtgcgactgc tctcggacat cgcaccgatg
     9481 ccagtgctcg cttcgagaag tcacaagatc cgcataacac ggtcatcgga atcggacgtt
     9541 tcgtctacct ggcacgtccc gaactcccgg acatgaagtt taccagccgg ctctctgact
     9601 gtttcccgaa cccgcccaaa ccgatcacga tccgcgtcga tccggcgttt gtcggccggt
     9661 acatcggtcg ccaggtgccg gccgaccgga tcatgagcat cctggcaaaa atcggtttcg
     9721 ccgtaaagcc ggttgacggc atgcttgaag tcggcgtgcc gacttggcgg gcgacaaaag
     9781 acgtcagcat cgaggccgat atcatcgagg aagtggctcg gttcatcggt tacggcacga
     9841 tcacgccggc gttgcccgag gtcaccgtcc gttacgccga gccggaagcc atcacgcgat
     9901 tggagcgcca gacgctcacc ctgatgtgcg gcggcatgag ttatgccgaa gtgcatcgtt
     9961 acatctggtt cgacgccgag tggctcaagc tgctgggctt cgatcccggt ccgacgctgt
    10021 cgctgaagaa tccggtggcg gccgggaccg aacggcttcg taccacgttg ctgcccggtc
    10081 ttcttgccgc cgtcgatctc aaccgtcgcc actttgagcg cttcgaactg ctcgagatcg
    10141 gcagcgcctt gccatacgga gaaaaagaag cgcccgaaaa aaccgagcaa cgccacctgg
    10201 cgctggccgt ggttgccccc ggtcgcaagc cggcacatga ggacgccctg atcctccggc
    10261 tcaagagcga tttggagaca tttgcccaac aggtgctcaa aggtcatttt cagttcgccg
    10321 ccgaaaaagc cgccgcccca tgggagcacc ccgctaaaac ggccgcggtc aaacttgatg
    10381 gactgcttgt cggacggatc accgctgtcc cggcagcgct gcgccgccgc atcgatgaac
    10441 atttggttgc ctggtcaatc gccctggctg agatcaacct gtcgctcctg gccgaacgtc
    10501 gccccgaacc tcgcaagctg gctcccgtgc ccgtctaccc gcgcatcaat ctggatttct
    10561 ccgtgctcgc tcgatcctcg cgacgctacg aggacatcga aggggcgctg cggacgtacg
    10621 atcacccgct cttgcggcgc ctggctttcg ttgacagcta cgagggcggc tccgtacccc
    10681 agggtatgcg aagctttact ttccgggcca ccatcggcga cccctcgcga acgctgaccg
    10741 aacaggacat ccaggatttt cgttcgactt tccttgagtg gatgaagagc caggatctgg
    10801 ctctgcggac atagaacatg aaacagccgg ttcaacgccc cgtttccggg gctcctgcag
    10861 tcctatcttg cgttgccgca ctcgtgctca tgccgcggcc ggctgcggcc cagatgctac
    10921 cgccaagcac catcgacgcc gacaccgtct ggagcggggt catcgagatc aagggagagg
    10981 tatgcattcg agacgcggcc gtgcgagtcg aaccggggtc aacgatccgg tttgtcggcg
    11041 gcccgggatc aaacggcgct tccatccgtt tcggcggcag cttgtcgggc agacggttca
    11101 gcagtcgaag ccgtctcacg ctggcaggca ccgccgagcg ccccattacc gttgagactc
    11161 cggcgggcca tccgcccggg ttcatcagcg gcgacctgga gtccggctgc gccctggttg
    11221 cgcggcacgt cgtgtttcgc ggcctgggct cggccgtctc cggcaaacga ggcacgccgg
    11281 ccgtgtttct cattctcaat tcgcccgaca gcgatttgtg gctgtcacat tgccgattca
    11341 ccgactgcgg ccgcattcac gtggagttca tcggcggtgc ggccacggcg gagatcgaaa
    11401 gttgcacctt cgtccgcacc atcggcgaca caaccatcga attcctggga accggagagg
    11461 ggcttcgcgt catcagcgac aacatctcca gcgcggcgtt tcgcatcgag tgcccgcaga
    11521 cgttgctgaa gaacaatgtt ctggtcggcg agtgggccgc catcagcgtt aacgccagga
    11581 acgtctccgg aatcgcgatt acgggcaatt atgtcaactg cgcgaccgac cgcgacgagg
    11641 gccgttacgc gctgcgttgt tccgccgaag atgccgtcgt cacccgcaac gtcttgcttg
    11701 gcggcacata cgtcatcgaa caatcgcctc gcacggtgac cggcaatgtc ctggtgggcg
    11761 cggcagcgct tcagcccggt gtgagcccgg gtggcgcgcc cgccacgaaa ctcaccacca
    11821 cgcattattt gctctcgaaa ccggcaccgc aggccaggat cgccgacaac ctcttcttgg
    11881 gccccgccta cgccgcgatt tccgccggtc gcgagagccc gcagatccgc attgagcata
    11941 atttcttcga cggctggggg gtggccaaac gggcggttca tttcaatctc ctgatgcccg
    12001 cttcacggga agcgcctttg gccgccggca tcaccggcaa catcttcgcc cgatatcgcg
    12061 ccgcccccgt ggcggatgac gccaagcacg tcggtacggc cgcgcaggcg ggtctgaacg
    12121 tatttgccga ggtgcctgat accttgtatg agctcagcgg tatccagaag gccgctcccg
    12181 gcgacctggt acttcctgcg ctcgccgatc ttcggctcca ggcaccggtg gcgaccaggt
    12241 cggccctgtc tgccgaagcg cagttgctgc agcggcagct cacggttgcc aaggttcgcg
    12301 agatgtggtt cgatgcatat cggcccgtgc agggctcgcc gctgatcggc agccggcctg
    12361 ccggtccgcg gcctgactcg tctcccgaat gatgcacttg caggcgcgat ccgcaccgga
    12421 gggcacggtc gaagccgcca ccaggcggcg caggcctgcc gcgattgtca ttgaaaggac
    12481 acgatcatga ctcacgggct tcccgaaaac gccgcgttct cccggcgccg gcttcttgtc
    12541 ggcggttgcg ccgccgtcgt cgggcccgcc accgttcggg gatccgtaca acaagctgcg
    12601 acccgacacg cgcagggtcc ggatacctcc tcctggccca aaccaatcaa ggccttttgc
    12661 atcgacttca actgggccgg caaccagcca tcgccgccgg gaacctacgc ccacgcggac
    12721 ccggccgacc atgtccgctg gtaccgggac ctcggagcca ataccatcca gaccttctgc
    12781 gtcacctaca acggctacgc gtggtacccc agcgacgtcg ccccggtcac cccaggtctc
    12841 aagagcccca acttcctcgg cgatatggtc gaactcgggc acaaggccgg gatgagagtg
    12901 ttgggctact tcacgctcgg ggcgaatcca tactgggaga agcttcatcc gaacctcgta
    12961 catggcggcg attccgactc catcaagatc cccatgacgc tcgagtacct cgactacttt
    13021 tgccggtcgg tcgaggacgc gatcctcaag accggtgtgg acggcttcat gatcgattgg
    13081 gttcgcccga cgcagcataa gaactggctt gactgcgaaa aagagatgta ccgccagctt
    13141 ctcggcgaag aattcccgga gtcgggcagg ccgtcgacca aaatgatact cgagttcgac
    13201 cgccgggcta tggagcgggc ttggcggcac atccggtgga cggtgcgagc caccgggccc
    13261 tccgtcatct ggacaaacca cccgttcctc aagagcgagt atccgatctg gacgggccac
    13321 cgtgtgctcg aggaagtgga ctgggtgctc aacgaagctc cggaacttga tcacctcgac
    13381 tggctgcggc ggcaggtcgg gccgcggacg ctgatccttc aaaacctctg cggctggaag
    13441 gatcatgatg ccttgatgtg gaagaagatc gcccccatgg ccctgggctt ctacggtttc
    13501 gccaaggccg acgagaagac caccctgccc gacgacacaa tcgaggtgaa cgtcaagaac
    13561 atcgaaatct tgcgcgaggc gtaccactcg ctatagaacc ttaccgccag atatctgtgc
    13621 ggcttgcgcg catgtttctg gatcgcagaa cgtttgggtt gcggcccgca aaaaggcggt
    13681 ccgccttggg acgactcccc tttgctcgcg gggatcgttc tcctgttcgg ccgtcggtcg
    13741 ggcctgtgcc cgtgtctcgc cgcgccgaag cgtggtcagc gagcgagcct cttggcaaag
    13801 cccggggccg ccaatgcaga ccctgccgac aaactgtccg ccgcagccgc tttcccattc
    13861 gcatcgtcct gtttacactg gtacacacgg cgaacgggcc gatcggatcg gctcgcccga
    13921 gacggtgact tatgacacgc aagatcgacg aggcccaggt caggcatatc agccatctgt
    13981 cgcggctgaa tccgaccgaa gatgaggttc ggctgtttag cgagcagctc tcggccattc
    14041 tcgagtacgt cgagcagctt aacgaggtgg acacgaccga cgtgccgccg acggcgcatg
    14101 ccctgccggt gaagaacgtc tttcgggccg atgagcccag gacgggtctc acgccggacc
    14161 aggccctcgc caacgcgcct caccgtgagg gcaacttctt tgccgtgccc aaggtgctgg
    14221 accaggacag cgcgtgatcc tgcgacgggc aagaccgcga tcaggcaacg aatatgaccg
    14281 caccaacgct gctggaaacc cgtgactcga tccgcaaccg caagggaacg tcgcaggctt
    14341 gcgtccggca catgctcgac gccatacgcg aactggatcc caagatccgg gcggtgatca
    14401 gtctggacgt cgaacgggcg cttcatcttg cccgccaggc cgacgagcgc atctctcgcg
    14461 gtcagatcga cgggccgctg gccggcgtgc cgctgctgat caaggataac ctgtgcacgg
    14521 cgttcggtcg gaccacctgt gcctcgcgga ttctggaaaa cttcactgct ccatacaacg
    14581 ctcacgtcgt ggaaaagctc gaagccgccg gcgcgatcat cgtcggcaag accaaccttg
    14641 acgagttcgc catgggttcg agcaccgaga acagcgcctt cacggtcacg cgaaatccgt
    14701 ggaaccttga ttacgttccg ggcggaagtt cgggcggctc ggcggcggct gttgcggccg
    14761 ggatcaccgc ggggagtctc ggctcggata ccggcggatc catccgccag ccggcttcat
    14821 tctgcggggt gaccggtctg aagccgacct atgggcgggt ctcccgctac gggctggtcg
    14881 cctacggcag cagcctcgat cagatcgggc cgttcggtcg gaccgtgcgc gacgtggccc
    14941 tgctgctcgg cgtcatcgcc ggaaaggacc atcgcgactc gacgagtgtc gatcaatcgg
    15001 tgcccgatta tctggcggcc ctggagcagc ctctggccag cgtgcggatc ggctatgccg
    15061 aggagtactt cggcgaagga ctcgatcccg aggtgcggtc tgccgtcgag gcggcgatcg
    15121 aattatacag atcagccggc gccaccgttc agaccgtgca tctgccgcac atgaagtacg
    15181 ccatcgcctg ctactacctg atcgccaccg ccgaggcctc gagcaacctc gcccgctacg
    15241 acggcgtcca ctacggccac cgcaccgcct cgcccagaga ctacatcgac atgtactgct
    15301 ccagccgcca ggaaggcttc ggtgccgagg tccggcggcg gatcatgctc ggcacgtacg
    15361 ccttgtccag cggctactac gacgcgtatt acctcaaggc tctcaaggtc cgaaccctca
    15421 tccaacgtga tttcgagcag gctctccgac aggtggatgt gatcgcctcc ccgacctcgc
    15481 cgacaccggc cgtgcgcatc ggcgagaaga tcaacgaccc gctggccatg tacctgatgg
    15541 acatctatac ggtttcggcc aatctggccg gaatccccgc gatcagcatc ccctgcggct
    15601 tcagccggtc cggcctgccg atcggcctgc aactgatggg tggccacttc gctgaagaga
    15661 aactgctgcg catcgcccac cagtaccagt tgcggaccga ctggcacacg cgaatgccgc
    15721 cgatcgccaa tggtggctcc tgatctacgg gtaggctgat gacctcttcg gtttaccatg
    15781 cccgatggat tctgccggtt gcctcgccgc cgatcgaaaa cggccgcctg gtggtgcagg
    15841 atggccgcat cgtcgcggtt gagaaggccg gcacgcttga tcccgcggcg atcgatttcg
    15901 gcgacagcgt cctgcttccc ggttttgtca acgcccacac gcatctcatg ctcacaggct
    15961 gtcgcggcct ccgttatcac ggttcattta ccggctggat ccgcgatctg gtgacggtcc
    16021 atccgcggtt gagccccccc ggtacagtgc gccagtcggt gcgcgagggc ttgcagcagt
    16081 cgctgacggc cggcgtgacc acggtggccg atatcggtga cggcatggag gcgatcgaag
    16141 catgggcggc ccgccggatc aacatcgccg gcttcctgga agtgctcggc atcgggccga
    16201 aacgtgccgg aaaacacccc agcgcagtgc aagtcgcagc cgacatgctt cgcaacgggt
    16261 ccctgcccct ttgcgccgag gcacaaaaga agggatcctt gactcaggag agcctgttcg
    16321 tgcccggcct ttcgccgcac gcaccgtaca gcacggatgc cgacgtctac cgggaggcga
    16381 tcgagtgtgt ctccgggctg tctggcattt gtcgtggcct gtgtacccat ctcgccgaga
    16441 cgcccgagga agaacagttc ctgcgtgatg gcaccggacc tttccgcgat ctgctcgagc
    16501 agttcgggct gtgggacggc tcgtaccagc cgcccggctg ctcgccggtg gagttcatgg
    16561 agcacgtcgc gtttctttcg caccggccgc tgctggttca ctgcaactat gtgagtgatt
    16621 cggatctcga cttgatcgcc gcctcggagg catcggtcgc ctactgcccc cgctcgcatg
    16681 ccttcttcaa ccacgagccg caccgctggc gcgagatgct tgctcgcggc atcaatgtct
    16741 gtgtgggcac cgactcgctg gccagcaacc atacgctctc cgtgctcgat gaattgcggt
    16801 tcctctaccg gatcgatccg tcgattcctg gcgaacagct catccacatg ggtacgctgg
    16861 ccggcgccaa ggccctgggg ctggcggatg cgatcggttc gctggaaacc ggcaagagag
    16921 ccgacctggc ggtggtcacg ctggatcgcc cagggtcacg agatcccgtt gatgctatcc
    16981 tcacgggaac ggcccagcct tcggcagtgt ttgtctgcgg ccggaacgtc caggggatgc
    17041 ggtgataacc gctttgagat tcgggcatgt gtctgagaac gaatcagaat ccggttgacg
    17101 cttcaagaca gcctacctat gatcccgcgt gaccggccct attgaggcct gttctgtcat
    17161 tggagcgatg gaatggccag actgtacgac aacattaccc agacgatcgg caatacgccc
    17221 ctcgttcgta tcagacggat catccaggcg cccgcgacgg tctacgccaa actcgagttc
    17281 ttcaatccgc tgtccagcgt gaaggaccgc atcggcgtgg caatgatcga ggcggccgag
    17341 caggccggca ggatcaacaa aaacacggtt ctcgtcgagc ccacgtcggg caataccgga
    17401 atcgctctgg cgttcgtctg cgccgccaag ggctatcgcc tgatcttgac catgccggag
    17461 agcatgtcca tagagaggcg gaacctcttg cgggccctgg gcgccgaact ggttttgacc
    17521 ccggccacgc aaggcatgaa gggtgcgatt gccaaagcgg aagagattgc agcccagact
    17581 ccgaacgcct tcatccccca gcaattccag aacccggcca atcccgaaat ccatcgcaag
    17641 accaccgccg aagaaatctg gcgcgacacg gacggggcag tggatattct tgtttcaggg
    17701 gtgggtacgg gcggcacgat caccggcgtc gccggcgtga tcaagcaacg caagccgtcg
    17761 ttcaaggcca ttgccgtcga accggccgat tcgccggtca tcacccagag gcgatcaggc
    17821 gaggaactca agcccggccc gcacaagatt cagggcctcg gcgcaggttt aatcctcggc
    17881 gtgctcgatc tctccctgat cgatgacgtc gtacgtgttg ccaacgaaga cgccttcgcc
    17941 tgggcccgca aggccgccag acaagagggc atcttgtgcg gcatctcctc aggcgcggcc
    18001 ctgtgcgccg ccaacgaggt tgccaaccgc cccgaaaacg ccggtaagat gatcgtcgtc
    18061 atcctgccca gttgcggcga acgttacctg tccacgccgc tgttccacga aggataaggc
    18121 ggttgccgat cgaacgtacg ttgcttgggg gccgacgtcc tttccgaacc tgctatgtga
    18181 cagaggtctt ccagccgcag cgcctcgaag ggtcggaggc aagctacgcg gtcttttcga
    18241 gtgccgccgc gtcactctga gatgcgcagc accaggatcc gcaaccttgg cgatcaatcc
    18301 tcctcgtccg catcgtcttc ctcgtccggg tcttcccact ctccgtcatc atcctcgtcc
    18361 tcgtcgcttt cgtcgtcttc gtcctcgtcc cattcctcat cttcatcttc cggatcggga
    18421 tactgcggag ggctctcacc tacgcgttgg gttaccctgg gatactctcc gggggggatg
    18481 ttctcctcga tcgagatgac gttgatctgg tgccaccagt cgtcgccgta atcgaaccaa
    18541 tagccgaatg catctccgac tttcaggcca agagtgccga ttctggttcg agtcaggtct
    18601 cctgcctgtc gtctgtcgta cgcatcacgg gcatccggta agacgtagca gcgcgcgccc
    18661 gggtcctggg gccccttgcc gccgatctga aactcgtaca tgtgctcgtc atcacggtca
    18721 aaggcgtcga aaatggcaaa gtgcagcttg tccaacgtct gatcgccgcg gatttggatg
    18781 gtgcgggaga caaccgggtt ttccttgacg aactcctcgg tgacgggccc accgattaaa
    18841 aatacctcca atgtatacag tccgctcccg gacgaggtct tggggttttg tttcttcgtc
    18901 tgacgggtag ccatgacaga tctccaacat tcagtgaacg gacccaacac agaccacgaa
    18961 ccagagcata acttctcggc atgcaagacg ccagagacga ccgacgaatc aacccgtttg
    19021 catccggccg gcgcggcccc cgccgcaacc ctgcagcacg cgccctgcaa cccgtgaagg
    19081 catcagcatt tagagcttgt tctccccggc ccgcgtttgt tcttggtttg gggctagaat
    19141 aagggaaagt cgtggtcgat cacgacggtt caagtcccgg gactctccga ggaggtcact
    19201 tcgatgagat acgtcagccg caagctgggt ccgctgctca ttgcgattgt cgtcgggttg
    19261 ctgccggccg gttgcgcgtc gacggagacg gtcgggcccg ctgcctcgct ggaagcacgt
    19321 accgatctga ttcccgtcgg gcagaaggct cctgatttcg acgctgccga ccaatcgggc
    19381 aaacgcatcc gcctggccga gctgctcaag aagagcagcg tcgtgctgat tttctatccc
    19441 ggtgatttca cgccgggctg cacgagacag ctttgtgcgg ttcgtgacga ctggtcgcag
    19501 tttgaaaagc acgggatcac ggttcttggc gtcaatccgg ccgacgccgt gcagcacgcc
    19561 cgcttcgtcg gcgaaaaccg gttccccttc tcgcttattg tcgacgaggg ttcaagcatc
    19621 accgctgctt atggcgccag gggaccggat agaacgcagc gcacggtcta cgccattcgt
    19681 aaagatggca aggttgccct ggccgagcgc ggctttgtcg cccatgagaa gattttcgcg
    19741 gcgctcaagt gacggccacg accgatgaac cggccgcgtg gccttcagaa ccttgcggcc
    19801 ggatatctgt gcggcttgcg cgcatgcttc tggatcacaa gtgcgcttgg gttgtgaccc
    19861 gcaaaaaggc ggtccgcctt gggaaccacg caatgggctg tgtttgcgca cccgtgccgc
    19921 ctctactact tgccgccggc gcggaccggc tcctgatcgg cttctttggc atgagcccat
    19981 ctggtcagac gcttcaagag cgaacccata ggcgtgggat ggatgacgcg gtcgattgtc
    20041 tttgttccgg gatggtccgg agccacctcc aacagcatct tatagtatcg ctctgcaatc
    20101 tcatcttcac cgatggccga aaaatacctg gccatgagac cgttaaaggc cacatattcg
    20161 cttacgtgat atcgctttcg atcggtgatc cagaggtgaa tcgaaaagcg attgtccagt
    20221 atctgctcca ctcggcggat atgccaccga cgaagcagcg catgggcata gttgatcatc
    20281 gcaaagagat agttcgggaa ccggctgtac gcctcctcag ccatttcctc ggccttggcg
    20341 tacagaccgg cattggaata ggctatcgtc aagtaattgt agactttcgg cacctgcgga
    20401 aactcccggg ccagccgttc gagaatcggc acctggtccg tggccccttc catcactctt
    20461 gagccgacct ccaggatcat ctcccgatcc tcgggacgga gacgatccaa tgtcgggtca
    20521 ggtatcgggt cttcggtgat ttcgaaactg agcatttcca ggacaacagg ctcgcctttg
    20581 tgcccgccgg tggcgatcag acgcagtccg gaaggagaag gcggacgctg tgatcgaagc
    20641 cttcttcgaa gtcttcttga tgacacgtgc cggtgtcttc gagacatgaa ggtcactcct
    20701 tgacgcgagg gcccgcttcc aagctgacgg accatagtgt gccttgcggc ggtctccacg
    20761 tcaacggctg cttcgacaag aacaggccaa aaaacctatt ccgaaacccc gaaaaccgac
    20821 cggctggagg ccagtccccc acaggtcagc aggccttccg ccggcggccc caattcgagg
    20881 atttcctggc gtttctgata gagtttggcc cgctgcccgt gcaccgcggc cagccgggca
    20941 acccggtgct cgctgcaata tgaagtgttg cccacggccg gacgccgcgg atgctcgacc
    21001 tcgatcccgc tgcgggtcac gatccggacc cggtcgggat acaggaagag cgtgccggga
    21061 atgccgatgg cggtcggggg catggaatag cggatacgct cgtactccac gaagccggtg
    21121 gtgccgacgg tcactgccac ccgcagggga tagtcatccg ccggggtcgc caggggcgtg
    21181 aggcgttccc gctccgcctt catccgctcg gccgggatct ggttggtggc ccggctgggg
    21241 cgctgcaggt tgacctcttc cagccaggcg atgagctgct gctccaggtc cgcgcggtcg
    21301 tggaaccggc ggaccttgaa gaagctgccc ttgacccagc ccaccaggtt ctcgacggat
    21361 cctttctggt tggcccgccg aggccggcac aactccaccc cgaaactgta gtccacgggg
    21421 acgtggacga aaaccgggtt ccagttgatg aacccctggg cacggctgag aacgatcgtc
    21481 ttgggattgt cgaaaaccgc ccgcaggggc acaccgccga aggactcgaa ggccttcagc
    21541 aacgcccgga tgagcgactc gactcgctca tccgggacga tgaccacgtg cgtccatcgc
    21601 gagtacttca gacgagaagc gaagaagtgg atcttctcgc tcgtgccgtc ggcataggtg
    21661 acgttcacac tgccgaaatc atgttggctg aactcgcccg gcaccccctc gaaccgcacc
    21721 tcaggcccct ggttcttccg gggacgaacg gacctgacca actcgtacac cgccgacttg
    21781 cccccggtgt aacctagcga tcgcaggcgg ctgaacactt ccactgtcgg cagcaacggc
    21841 tcggcttcca ggatccgttc ggcgtcgccc tagaaggcct ccacccggct cggacgcccg
    21901 atctgccggg accccggcgt cggggccgac tccaggctcg tgatcggcgg ctccttcagg
    21961 atccgctgta ccgaacgttt cccgactttg accgcggccg cgatctcctc caacgtgtgt
    22021 cccgcctgcc gcaatgccca aatctttacc cggtccaaca tactgatcat cctcctcgtt
    22081 cctcgcggcg gggagcccca ttgcccccgg ccattgcgag gcaaaactgc cggaaatcac
    22141 aacctcacat gtggtcagaa tttccggaaa ggaggtggcc agaatctaag gaactggcag
    22201 cgcctttggc ggggaaaccc gctgaacggc cttgatcgcc caacctggac cggtcggcat
    22261 gcccgcccag actgtccgca cccggcatgc acgggtctgt gacagctcag gcggggagac
    22321 gctatggctt ttcagaaccg caaccgtgag ggagcggccc catgtggcat tggccgctct
    22381 gcggcttggc ccctcgctta cgctcggggt gctgaaagag ccgcctgaac ggtcatggat
    22441 tcggcatgca cccaaggccg cttgccccgt tgaacggcgc ctcccgcaaa ggtatcctag
    22501 tccgttgggg aggtggcagg cgtccgccaa acttggctat tggtttgggt tgccgacgcc
    22561 tggttcgggc cgttgatggt gagcgtagct cagttggtta gagcacgagg ttgtggtcct
    22621 cggggtcggg ggttcgagtc ccctcgctca ccctttttcc cgcttggtgt ccgcgcacga
    22681 acggaccggg cgcgaacgag ttaggtcgag cgtttcccac ccatcggttg tcgcggacgt
    22741 tccttccacg ggtgatcacg tgcctgcttt cggcaggcgg cgcaaaaaaa aaccgccggc
    22801 cgcgtttgcc ttggccggcg gtttgaaacg tcagtcggat aggtgtgtgc aggcagcgac
    22861 ttcagcatta ccgcgctcca tcttcgagcc tcgcggtgta ctcagcgaat gacggcctaa
    22921 aaacagcaca tcattcatac gatcaagcga ttggccatta gtaccggtcg gcttaagaca
    22981 tttctgtcct tacacttccg gcctatcgac ctggtagtct tccaggggcc taccgactcc
    23041 gaagagtcgt cgagacctca tcttgagggg ggcttcacgc ttagatgcat tcagcgttta
    23101 tcctttccca gcatagctac cctgcgctgc cgctagcgcg acaacaggta caccagaggc
    23161 tggtgccttc caatcctctc gtactaggaa gacatcctct caagtctcat acgcccacgg
    23221 cagataggga ccgacctggc tcacgccggt ctgaacccag ctcg
//
LOCUS       DAOTPW010000066        23237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2267_length_23237_cov_4.965620, whole genome shotgun sequence.
ACCESSION   DAOTPW010000066 DAOTPW010000000
VERSION     DAOTPW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2267_length_23237_cov_4.965620"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..990
                     /locus_tag="P5316_07915"
     CDS             193..990
                     /locus_tag="P5316_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84920.1"
                     /translation="MRYRLSTISLLAGFVAPASGAVTMSVLTAGGSGASVTVAPGGVF
                     SVDIRLDIQDVVVVAAQAQLVAGAPNVLDITGGSYNAADWDASWGLPIDPMGLDPVGS
                     GTIGSLPAGDHIGPTAVTVLATIHLSVDPAAPAGTYTLNLINASVGDDTFTDIDAIVG
                     PDFIVTVSGSAPPGGGGTTPPDDGGIAPPDDGGTEPPDDQGTEPPNDDGTDPPDDGDT
                     SSTGDGGTTPNGGNATIPTVTRCGAGMIETLSLSLTALLARPRRRLR"
     gene            1129..3282
                     /locus_tag="P5316_07920"
     CDS             1129..3282
                     /locus_tag="P5316_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRR84921.1"
                     /translation="MTSVTGPSAACRILWFFVRLCLTALGVMVVAGAILRWDRLHSLH
                     DPRWQIALTLVGLAIGGWLAGLQARGAYRYPWIAAISVATIVLSQTCYLVLVWTSWKM
                     HPLIWRVWWLSMIAACHSTHIIWLRMAAPPDRRGLVRVTIACALLTSLLLAVMGLRRH
                     LLADVAPLHIWMTALAAGMATLGSVVLWLRGIHLSAARVPPTVKIAWLLVGQALLVLA
                     AFYAGRVTAPSPPLFEPLPSLLANLSPAELESQARSDLDRLKIVVAGLDDLIAKGSAL
                     QSQLQQRRAADSRQYFTPDEEDQLRRQFMSYLSYRAALLRMVATYAGFQSVRDPALRA
                     RCMLLGYAAGTGLFDADRRFVSVYGDDQPARRKLNEADPEAGIPAGMLDRIRESVFSG
                     RNAQALAEMEAYFETHREVWASGQVWPAEDWFWIEKLIDRSAGQIRQSALDRAGARFE
                     QILARVRQDSYTPAYAAQSVVSTWIGDTRLAQWEPLISREQILAIQEQLRPGDILLER
                     RNWFLSNAFLPGFWPHAALYVGATEDLERLGLIRREGNRYTSDDPMIRDHLTRYLAMA
                     ADGQPHTVIESVSEGVIVNSLTESLHADYVAVLRPRLDDAQKARAIARAFAQLGKPYD
                     FEFDFFSADKLVCTELVYRAYEGLIHFELVRVMGRDTLPALEIARKFAKQRGTPDRQL
                     DFVLFLDRVPGEHRSAPSDEEAFCSSIGRPREFNE"
     gene            complement(3830..5023)
                     /locus_tag="P5316_07925"
     CDS             complement(3830..5023)
                     /locus_tag="P5316_07925"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRR84922.1"
                     /translation="MISRLSMWVFVVGVGFASLPVLAESPTSQPTREMVAVEGKGYLE
                     QVDGYPVLHLKGSPEEMGYQHGVLMREHIRQNVAFLLDQNPDEVIEIGPLKLTRTMIG
                     GMLNGVFADKVPERFMKEMNGLARGAGLPVQRITAANLIPELFHCSGFALLKEATAEG
                     RLLHGRVLDYGVNLRLQEHAVLVIQEPDGQIPFVNVSYAGFIGSVTGMNFEQISIGEM
                     GGRGQGQWQGIPMSFLVRMVLEQAKTLDEAIAVFEKNPRTCEYYYVISDARANTAVGM
                     KAVPEKVELVRPGEPHPLLQSPVKNTVLMSAGDRYQALSRLVAEGYGKFTQASAIRLM
                     DAPVAMKHNLHDALMVPGQGIIWVANADQEGSPAWKQRYYRFDCRALLKSRPPAIAPH
                     DAAKR"
     gene            5158..5304
                     /locus_tag="P5316_07930"
     CDS             5158..5304
                     /locus_tag="P5316_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84923.1"
                     /translation="MSGVGVPLAAGVSRCACLDADGDAEVDQSDFAEFQSCFGGENQM
                     PACP"
     gene            5386..6582
                     /locus_tag="P5316_07935"
     CDS             5386..6582
                     /locus_tag="P5316_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRR84924.1"
                     /translation="MRATKACIVMLMVATFLAASSASQAVPGQYEAELVFPLHPQHNH
                     APGIVECPNGDLLVSWYRGSGERSADDVAVYEARRRKGSTQWSEAFLLADTPGFPDCN
                     TAMHIDGQGRLWLFWPTILANSWESCITNFRVASHYTGEGAPKWEWQGVILLKPRAFR
                     EKMHRALDERLRAEPSILPDEVQTKQKIEQLKERIDDKLFSRLGWQPRCKPTTLPSGR
                     ILLPLYSDTYSAGIMAISDDKGRTWFASEPLAGFGSIQPTVLRRSSGTLVAYMRENGP
                     LKRIRVAESADEGITWGPVGVTDLPNPGSGIDGLRLANGNWVLVYNDTTEGRSSLAVS
                     LSSDEGRSWKWTRHLERHKTGSYHYPAVIQAADGRIHVVYSFFTDGGKSMKHAAFDEA
                     WISEQD"
     gene            6701..8227
                     /locus_tag="P5316_07940"
     CDS             6701..8227
                     /locus_tag="P5316_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRR84925.1"
                     /translation="MTLRFMDVGTIVVYLLGMAAIGVYFARKKKTTEGYFVGNRAFPG
                     WAVGLSMFATSISSVTFLAFPGAAFILDWRQIVQNLTLPLIAVVAILLFIPFFRRGKV
                     MSAYEYLEERFGPLLRLYGAINFIILQFVRLGTVLYLVSIPVQILTGTSIIWVIVIVG
                     VVVSFYTILGGLEAVIWIDVVQAIVLITGAVLPLFFIVDQLPGGLAQIVQDGMKQHKF
                     SLGPMSWDLAERTFPAMVLIGLLNWTTEYTTNQNVIQRYLAASSMREARKATAICAAM
                     SLPIWVFFFFVGTSLFVYYQVFPDSGIEQIVSERRTDYIFPYFILSRMPAGIAGLVIA
                     GLLAATMSLDSSINSVATVTTVDIVKRHICPGRDDAFYLRVGKIASVGAAVAMIVGAV
                     VIAHLPKESMVDLNLRIIQIVFGGGLMGLFLLGFLTTRANYISASVALVLTLLLDAYL
                     ALCSFDQLPDCISVRVHTYWIGVIANGFVIFVGYAMSFITPGVPKPLGRLTIWTMGSP
                     "
     gene            complement(8367..9434)
                     /locus_tag="P5316_07945"
     CDS             complement(8367..9434)
                     /locus_tag="P5316_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR84926.1"
                     /translation="MARILIVEDEEGFRRQLDIGLSALGHEIRCASNGREGVDVGARF
                     RPDLLVTDWMLKDDIHGLHVIQVLRAVLTDLRAILITGFASTELRQNVDSSGIAQFIE
                     KPFTLEQIRKAVENALTGPYQTGRRATLAVVEITTGGGIRHANDAALDLFDETEKGRG
                     ARNFSELFPAGAAPDLDQALDDWKVAHPVADRRIAWHLRTQEPDEHGSRLVILRRGDG
                     PQYVGLPLIEMLLGVVSPRGTQWPFDGRVVIIEADATTRKWLVTTIENVGAGCYAVES
                     VDQAVRLLGTDEGLQFAVLDYNTAGPDPSDAIEQMRAVRPDVILIGSSIDDRREAFAC
                     LGVTLFLQQPWRAADLINLLT"
     gene            complement(9535..11724)
                     /locus_tag="P5316_07950"
     CDS             complement(9535..11724)
                     /locus_tag="P5316_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HRR84927.1"
                     /translation="MTRSWERQWMEGELAELAGDRASDVEREFSHCVGQVRLVATYFA
                     SSEHVTPVEFRTYVSRLTQGLGQGLLLQWVPYVRQAERAAYQDRARAEGRQDFEFKDL
                     DAAGQLIVAADRPEYFPIYYEYPENRVPSFLGLDLSTDADKLALFRKACDAGEVIVLS
                     RGSHLPQSSDDPSFLVIVPVFHTPGRSDLPESHRQSLTGLVIGVLRVQDYLNRALRPS
                     GPRGVIVRIADSPEPGTSLSCWLYEAPGESVREVAPSAFRETLSTSCSQSRSVQIGDR
                     QWLVTCLSTPAFEAARQRVTQWILLILGLAITGLVAGHFRRTRDYTQGLMEVNERLVR
                     EIGERERIASSLKENERFLSILLANLPGMVYRCRNDRNWTMVFVSEGCVSLTGYLPAD
                     LLENAKTSYAEVIHPEDRLQVWDKVQAALAEGTEFELTYRIRTAGGDEKWVWERGRGV
                     FSAEGELMFLEGFVADITEQKRAQDELARYRENLEARVAERTRQLDESKEQLRRSERL
                     ASLGTFAAGIAHEINNPVGTMLLAAENALELRKMPGSEAVVDDCLRGIVSEARRCGAV
                     IQGVLQFARREQAPKTPTSIEAVIRRSVEMIAPQAQRQEATVSVHLGADLPQISLNAL
                     QMEQVFVNLLRNALEAGGKGVKLEVRADVADDRLRVTVSDNGPGIPRDHLPRVFDPFF
                     TTRRERGGTGLGLSLAHAIIVDHDGQISVNSRPGGGTTFTIDLPLRS"
     gene            12150..13847
                     /locus_tag="P5316_07955"
     CDS             12150..13847
                     /locus_tag="P5316_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRR84928.1"
                     /translation="MTSDDFSILIGGEAGQGLVTAGTILAKALMRSGLGVLVTQSYES
                     RIRGGHNTFAIRCAPRPVIAPRETVDLLLALTGDTVGIHQGQMTARGRMIVDTAHGAA
                     ESDSCVAVPFKELADKRMFTMAATGVAAALLGLERTLMGQTVNDVLGTKHPELTEANQ
                     KVLTDAYEWVGQNGFPKLGPPSARNRSRRMLMDGNEALAAGAISAGVRFCSYYPMTPG
                     SSIMQAMITHAKHVGVMVEQAEDEIAAINMAIGASYAGAPSMVSTSGGGFALMVEGVS
                     LAAMTETPVVVVVAQRPGPATGLPTRTEQADLEFVLHSGHGEFPRALFAPGDVEECFH
                     LTRKAFELSEKYQGPMFVLTDQFLADSLRSAEPFDAANLQTVAPLIASADVPGPYKRY
                     AITDAGVSPRLVPCETPHLVIADSDEHDEEGHLTEDLGIRVRMVDKRLRKGRGICGEV
                     VRPGHTGDDSPDLLLVGWGSTKGSMEEAAAVLRGRGQSVGTLHFSQVWPLVGDLFVPQ
                     LEKAGQVVCIESNATAQFARLIRRETGFHISRRILRYDGLPITPEYILRELEALECT"
     gene            13847..14674
                     /locus_tag="P5316_07960"
     CDS             13847..14674
                     /locus_tag="P5316_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRR84929.1"
                     /translation="MVRIEDYGEFETAWCPGCGNFGILKAVKQALVRENVAPHEVVFV
                     SGIGQAAKAPHYLNCNVFNGLHGRALAAATGIKLAKPSCRVIVESGDGCTYGEGGNHF
                     LAAIRRNVDLTVLVHTNQVYGLTKGQASPTSDLGFVTKTQPDGVKVAPFNPIATAVAL
                     RAGFVARSFSGMQDHLTEMIVQAMNYPGFALVDILQPCVTFNKVNTFAWYKKRCQEVP
                     PDHDPTDWQAAMRLAYEWQERIMIGVIFKCSRPVLTFPQHDGTPRPQALSESMQRYY"
     gene            14689..15990
                     /locus_tag="P5316_07965"
     CDS             14689..15990
                     /locus_tag="P5316_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRR84930.1"
                     /translation="MPKDPSDHGADVLIIGAGAAGLAAAVMAAEQHRESRIVLLEGTS
                     RPGSKLLLTGGRRCNLTNRTVTPQDFSGSNPHAIKKVLGAFTVTQTIEFFRSLGVLLH
                     EEEGGKLFPQHGGAHAVRNALLGRVESSGLKILTRQKVKRIEQSSNAFVVSTSESTFT
                     APRILLTTGGCSYPSTGSDGSGYELARSCGHTIIPPTPALVPLVLAGGFHRGLIGISH
                     DVELTLVVDGKRVKTAGSLLWTHFGVSGPAVLDISGHWLRGKLEGLRVEVTVNLLPGE
                     TAASLEQWLIRLASNHPRMSFHRALSSRMPARLAEAVLHGLDVDGTTAMAHLSRDTRR
                     HVRSALVAWPLPVVDSRGYEHAEVTAGGVPLKEVNPATMESRICPGLFLAGEVLDVTG
                     RVGGFNLQWAWSSAWVAARGLTVDNAALPRAEVSAHSGRHH"
     gene            16060..16800
                     /locus_tag="P5316_07970"
     CDS             16060..16800
                     /locus_tag="P5316_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRR84931.1"
                     /translation="MNSFASILWITAGLLLPGQPATQPAETRLARILIITGRDVPAHN
                     WRETTPVLREHLEQTKKFEVVVSEEPLVLESSALDGYDVILINYYNWQRPSITNKAKE
                     NLLKFVRDGKGLVCYHFSVRAFGDWPEYRNLVGRIWTDGSGHGPRGPFKVKITDPDHF
                     ITQGTSDFDADDELYAKLVGDAPVHVLMEAYSDWSKRTEPLAWTLEYGKGRVFSIMLG
                     HTAKDCRNPDFVRLLQRGTEWVAQKDSH"
     gene            16889..18457
                     /locus_tag="P5316_07975"
     CDS             16889..18457
                     /locus_tag="P5316_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRR84932.1"
                     /translation="MSKRITNCRLALPAVLFLWSSFAWAEVKPHALFSDNMVVQQGVP
                     INVWGTAKAGEKVTVKLSTQEASVVADGEGRWSVRLEAMKAGDALTMTISGDNTISFK
                     NMAVGEVWVASGQSNMQWPVRASTNAEKEIAEANYPNIRLFTVSRVVAGKPQTDVQGS
                     WSVCSPQTVGDFSAVAYFFGRELHKAIKVPVGLINTSWGGTPAEAWTSMAVLEGEPAF
                     KSILDSWDARFKEYPAAIEAHIAAYGKAVREWLKSADSAEAAGKPIAPPPKLDFPGDP
                     RTSPHRPAGLYNAMIHPLLPFGIKGAIWYQGESNAGRATQYRTLFSTMIRNWRKDWGL
                     GDFPFLFVQLAPYQREPANPPPGTWPELREAQLLTMLNVPNTAMAVITDVGEEKDIHP
                     KLKQPVGARLALAARALGYGERIEYSGPIYKDMKRDGSKIVISFTHTGGGLVAKGGPL
                     KGFTIASSDQKFVDAQAEIVGNTVVVSSPEVAEPAAVRYGWANFPVVNLWNKADLPAS
                     PFRTDDWPMVTAGK"
     gene            complement(18619..19962)
                     /locus_tag="P5316_07980"
     CDS             complement(18619..19962)
                     /locus_tag="P5316_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRR84933.1"
                     /translation="MGMTMTEKILARHAGRNSVTAGENVWVNVDVLMTHDVCGPGTIG
                     VFYEHFGRNAKVWDPSRVVIIPDHYIFTADSKAHRNIDILRSFVKEQGIKYYYDPDFI
                     QGEGMPSPYKDPTKTNYKGVCHIALPEEGHTRPGEVLLGTDSHTCTAGAFGEFATGIG
                     NTDAGFVLGTGKLWLKVPPTMRFVFDGDLPSYLMAKDMILTVIGDIGVDGATYRAMEF
                     SGGGVMGLNIEERTTLCNMAIEAGGKNGIVPVDEVTADFVRARTDKPWQAVENDKDAK
                     FCYEKVFRMKDLEPTVAKPHSPDNRDLARNLKGTKVDRVYIGSCTGGKITDFRAAAKI
                     IKGREVKVPTFIVPATTEVHAKMREEKIDGMTLEQIFINAGCAMGPASCAACLGGPGD
                     TFGRANEAIRVLSTTNRNFPGRMGSKDAQVFLASPYTAAATAVSGTITDPREFLG"
     gene            complement(20141..20542)
                     /locus_tag="P5316_07985"
     CDS             complement(20141..20542)
                     /locus_tag="P5316_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRR84934.1"
                     /translation="MIQTITVTTGERCQLVDVTSKVQKAVRETRLSSGYVICYVPHTT
                     AGITIQENADPDVIHDFLWKLADLVPHVEASYRHGEGNSDAHIKASLVGASQTILVED
                     GSLVLGTWQGIYFCEFDGPRQRKLHIRCVAG"
     gene            20653..21762
                     /gene="mnmA"
                     /locus_tag="P5316_07990"
     CDS             20653..21762
                     /gene="mnmA"
                     /locus_tag="P5316_07990"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRR84935.1"
                     /translation="MTDRKGKVVVAMSGGVDSSVAACLLLEQGYEVIGLFMRTGVKAV
                     LDEPACPADEATSNRPFRGCCSAADAADARDVAGRLGIPFYALDFKESFESIIDHFVA
                     EYVRGRTPNPCILCNDQLKFGRLAEYGRAADADFIATGHYARIVQHAGQARLCRGVDT
                     DKDQSYALFQLDRGILPRVMLPIGEMTKERVRDYARKRGLPLFDKRESQDICFVPDRD
                     YARLIRDRRPDAFRPGRIVDRSGRELGRHDGIVNFTIGQRRGLRLAMGKPVYVTAIDA
                     ASDTVTIGPREELARSELRASKVHWLIDPPTEIIEADVQIRYTHKAARAVVEPIGADC
                     VRIRFREPQYAITPGQAAVFYDGDVVLGGGWIDLE"
     gene            21772..22734
                     /locus_tag="P5316_07995"
     CDS             21772..22734
                     /locus_tag="P5316_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRR84936.1"
                     /translation="MQSPGVPTLVFLHQGAIGDFVLALSIVQATAARVRAARVVAIAS
                     ASSAGLAAGRSIINQRLFPDQVGLHTLFGEGAAADRRLRELLADASYILSFLGPPEGP
                     VHNALREATQARVISLDPRPSAETLDQRRHITVQWASDVAGAGFELTELEPPAIRMDL
                     PAESTDDNRSNVIIHPGSGGDAKCWPLDRFMAVADKLQDCRVKWMLGPAECESDAVSV
                     KSLRQRCDGQNEVLLIEEDFVKAVGHIAAADLYLGNDAGMTHVAAAMGIPTVAIYGPT
                     DPAVWRPLGTHVCPVATPQPGMAIDAVPATAVIAAVHARLAQLS"
     gene            complement(22788..>23237)
                     /locus_tag="P5316_08000"
     CDS             complement(22788..>23237)
                     /locus_tag="P5316_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84937.1"
                     /translation="ENRGQFPVIRSTPNTSPNPGEQPGGGGRLSAASSDPPRTPRPTS
                     PPPHILGRKDADLPTINGPIATPAAEPAIEAQDSARQFTTTATIGGATREPLPASQTI
                     LATPSLTISAQPGTHHLTLTAPNSTDPTHVTFPASASHSLQIAVDWQ"
BASE COUNT         4295 a         7035 c         7338 g         4569 t
ORIGIN      
        1 gagggaccgc cccgcccatg cgcaggcagc ccctcccgtg cgtttggggc cggttatgga
       61 cccctgcccg agactgctgc ccgcgacttc tccgccggca cgcgatgggc ctgccccaaa
      121 cggcgtgggc catcgtcggg aacggcgaga acgtccgatc acgaaccctt gcgtcacagt
      181 ggagagacga gaatgagata ccgcctgtca actatctcgc tgctcgcggg ttttgtcgcc
      241 ccagcctcgg gcgcggtcac aatgtccgtg ctcacggccg ggggcagcgg cgcgtccgtg
      301 accgtcgccc cgggcggcgt tttcagcgtc gatatacgcc tggacatcca agacgttgtt
      361 gttgtcgcgg ctcaagcgca gttggtagcc ggtgcgccga acgtcctcga tatcaccggc
      421 gggagctaca acgccgccga ctgggatgcg tcgtgggggt tgcccattga ccccatgggg
      481 cttgacccgg tcggctcagg tacgatcggg tcattaccgg cgggcgatca tattggcccg
      541 acggccgtaa ccgtattggc aaccatccac ctgtccgttg atccagccgc accggccggc
      601 acatacacgt tgaacctcat caatgcaagc gtgggggatg acaccttcac tgatattgat
      661 gcaatcgttg gtcccgattt cattgtgacg gtcagcggca gcgcgcctcc gggtggcggt
      721 ggaacaacgc cgcccgacga cggtggcatc gcgcctccgg acgatggagg caccgagccg
      781 cctgacgatc agggaaccga acctccgaac gatgatggca ccgacccgcc ggacgatggc
      841 gacacaagca gcaccggtga cggcggcaca acccccaatg gcggcaacgc gaccatacca
      901 accgtcactc gttgtggagc cggaatgatc gagactctga gcctctcgtt gacggctctg
      961 ctcgcgcggc cgcggcgacg ccttcgatag cctggccgcc aggccaaaac ggtcgaacga
     1021 atgaccgccg gcgacccacc ccggtacgcc cacaccagag catgttgagc ggttcaatgg
     1081 ccggcgatac catgccgttt gggtttcctg cagacatgtg aggatcgaat gacctcggta
     1141 accggcccat ccgcggcatg ccgcatcttg tggttttttg tccgtctctg cctgacagcg
     1201 cttggtgtca tggttgtcgc gggcgcgatc ctccggtggg atcgtcttca ctcgttgcac
     1261 gatccacggt ggcaaatcgc actcacgctg gttggcctgg ccatcggtgg ctggctggcc
     1321 ggcttgcagg cccgcggtgc ttaccgctac ccatggatcg cggccatcag cgtggcgacc
     1381 attgttctgt ctcagacgtg ctatctggtg ttggtctgga cgtcctggaa gatgcacccg
     1441 ctgatctggc gggtgtggtg gctgtccatg atcgcagcct gccattcgac acatatcatc
     1501 tggctgcgaa tggcggcccc acccgatcgc cgcggcctcg tgcgtgtgac catcgcctgc
     1561 gcgctgctga cctccctgct gctcgcggtc atggggctgc gcagacacct cctcgcggac
     1621 gtcgcccccc tgcacatctg gatgacggcc ttggccgccg gcatggccac cctgggttcg
     1681 gtggtcctct ggctgcgggg catccacctg tcggccgcac gcgtgccgcc gacggtgaag
     1741 atcgcgtggc tgctggtcgg gcaggcactc ctggttctgg ccgcattcta tgccgggcgc
     1801 gtcaccgcac cgtcaccccc gctttttgag ccgctgccgt ctctcctggc aaacctcagt
     1861 ccggctgagc tggaatcgca agcacgcagc gatctcgatc ggctcaagat tgtagtcgca
     1921 ggccttgacg atctgatcgc caaaggatcg gcactgcaat cgcagttgca gcaacggcgt
     1981 gccgccgaca gccggcagta cttcaccccc gatgaggagg atcagttgcg ccgccagttc
     2041 atgagctacc tgagctatcg ggccgccctg ctccgaatgg tggcgactta tgcgggcttt
     2101 cagtccgtgc gcgatccggc cttacgagcc cgctgcatgc tgttgggcta tgcggcaggg
     2161 accggcttgt tcgacgccga ccgtcgcttc gtttccgttt acggcgacga tcagcccgcc
     2221 cgccgcaagc tgaacgaggc cgacccggag gccggcatcc cagccgggat gctcgaccgc
     2281 atccgcgaaa gcgttttcag cgggcggaat gcccaggccc tggcggagat ggaagcttac
     2341 ttcgagactc atcgtgaagt gtgggcctcg ggccaggtgt ggcctgcgga agactggttc
     2401 tggatcgaga agttgatcga ccgctcagcc gggcagatac gtcagagcgc cttggatcgc
     2461 gccggggccc gcttcgagca gatactggcc cgcgttcgtc aggacagcta cacgcccgcg
     2521 tatgcggccc agtcagtcgt ctcgacatgg atcggcgaca cgcgcctggc ccagtgggag
     2581 ccgctgatca gccgcgaaca aatacttgcg atccaggagc aactccggcc gggtgacatc
     2641 ctgctggaac gacgcaactg gttcctgtcc aatgccttcc ttcccggctt ctggccgcat
     2701 gcggctctgt acgttggagc aaccgaagat cttgaacgcc tcggcctgat ccggcgcgag
     2761 ggtaacaggt acaccagcga cgatcccatg atccgcgatc atctgacgag gtacctggcg
     2821 atggccgcgg atggccagcc tcacaccgtc atcgaatccg tcagcgaagg agtcattgtc
     2881 aactctctga cggaatccct tcatgccgat tacgtcgctg tcctgcgtcc gcggctcgat
     2941 gatgcccaga aggcacgtgc aattgccagg gcgttcgccc aactcggcaa accgtatgat
     3001 ttcgagttcg atttcttctc cgcggacaag ctggtctgca cggagctggt ctaccgggca
     3061 tacgaagggc tgatccattt cgagttggtg agagtcatgg gccgcgacac gcttccggcg
     3121 ttggagatcg cccggaagtt cgccaagcag cgcgggacac ccgaccggca gctcgatttc
     3181 gttctgttcc tagaccgcgt acccggagag caccgttcgg ccccaagcga cgaagaggct
     3241 ttctgctctt ccatcggccg tcctcgcgag ttcaacgagt aggacgttcc cggcaacgac
     3301 gtgtgtgccc tcgccgtccg tgcattccgg ttacccttgg aagcgcgcgg ccgcatgaaa
     3361 ggcgaccgga accgcgggga gcatacgaca cttcttgccg gtctggaatc acgccaaacg
     3421 aagcagtgag gccggtcgtg gccatgacga caatctgatc gcagtcgtca ttcgggagac
     3481 ggcatactga tttcagagct acggtcagac cgctacggcc tacgacggtg agaacgccac
     3541 cggcaccgtc ctctcgcggg accatttcca ctatgacgag ttgcggcacc tgacggccga
     3601 gttccaagcg cacggggccc cggtgggtga aaagtatcct tccggcaagc ctgcggtagg
     3661 cgcgaaagat gggacggtgt gtgcgcggtg agttccggca gcgtcaggaa gtggggtgtt
     3721 ggctgctgcg cgtgtcgcgg taattccaga gcgtcctgca ctcggcatgg tcgtcgctct
     3781 gccgcgacgt ttgcttctgc ccggtgcaat cctcgtcgag agccggcttt caacgttttg
     3841 cggcatcgtg cggggcaatg gccggtggac ggcttttcaa cagggctcgg cagtcaaaac
     3901 gatagtacct ctgcttccac gcggggctgc cttcctggtc ggcgttggcc acccagataa
     3961 tgccctggcc gggcaccatg agagcatcat gcagattgtg tttcatggct accggggcgt
     4021 ccatcaaccg gatcgccgag gcctgagtga acttgccata accttcggcc accagcctgc
     4081 tcagggcctg gtagcggtcg ccggcggaca tgagcacggt gttcttgacc ggcgattgca
     4141 gcagggggtg cggctcgccg gggcggacca gttccacctt ctccgggacg gccttcatcc
     4201 ccaccgccgt gttggcccgg gcgtcggaga tgacgtagta gtattcgcag gttcgcgggt
     4261 tcttctcgaa gacggcgatg gcttcgtcga gggttttcgc ctgctcgagc accattcgca
     4321 ccaggaagct catcgggatg ccctgccact ggccctggcc ccggccgccc atttcgccga
     4381 tgctgatctg ttcgaagttc atgcctgtga ccgagccgat gaacccggca taggagacgt
     4441 tcacgaaggg gatctggccg tcgggttcct gaatgaccag gaccgcgtgc tcctggagtc
     4501 gcaggtttac cccgtagtcg agcacccggc cgtgaaggag ccggccttca gcggtggcct
     4561 ccttgagcag ggcaaagccg ctgcagtgga atagttcggg gatgagattg gcagcggtaa
     4621 tccgctggac cggaaggcct gccccgcggg ccagcccgtt catctccttc atgaaccgct
     4681 cgggcacctt atcggcgaag accccgttga gcattccgcc gatcatcgtc cgggtgagtt
     4741 tcagagggcc gatttcgatc acctcgtccg gattctgatc gagcaggaag gccacgttct
     4801 gccggatgtg ctcgcgcatc agcacgccgt gctggtaacc catctcttca gggctcccct
     4861 tcaggtgcag gacagggtag ccgtcgactt gctcgaggta gcccttgccc tcgacggcga
     4921 ccatctcacg ggtcggctgc gaggtcggag attcggcaag cacaggcagc gaagcgaagc
     4981 ccacgccaac gacaaacacc cacattgaaa gacggctgat catgaccgga atccacatgc
     5041 tgagaagaac accgcaaacc gacacgggca acagagacgt atgcgttggt cggcgatcat
     5101 acagtgatgc ccttcaaggg gtcaaggcgg ttgtaacgag taatgaaaga gggccgcgtg
     5161 agtggtgttg gcgttcccct cgccgcgggg gtgagcagat gtgcctgtct tgacgcggac
     5221 ggcgacgccg aggtggatca gtcggacttc gcggagttcc agtcctgttt cggcggcgag
     5281 aaccagatgc cggcgtgccc gtagcaggtg cgtcctcgcg cctgccgaac ttgacctcgt
     5341 gcagggattg cttttacctt gtttgagtga ttgcagagag aaatcatgcg tgcaaccaag
     5401 gcctgcatcg tgatgctgat ggtggcaacg tttcttgccg cttcgagtgc aagtcaggcc
     5461 gtgcccgggc aatacgaagc tgagctggtc ttcccgctgc atccccaaca caaccatgcg
     5521 cccggaatcg tggagtgccc caatggcgat cttctggttt cctggtaccg cggctcgggt
     5581 gagcgctcag ccgatgacgt ggccgtatac gaggcccgac gtcggaaggg ctcaacacaa
     5641 tggagcgagg cgttcctgct ggccgacaca cccggcttcc cggactgcaa caccgccatg
     5701 cacattgacg gccagggacg cttgtggctc ttctggccca cgatcctggc caattcctgg
     5761 gagtcctgca tcaccaactt tcgggttgcc agccactaca ccggcgaagg ggcaccgaag
     5821 tgggagtggc aaggggtgat tctgctcaag ccacgcgctt ttcgggaaaa gatgcacagg
     5881 gccttggacg aacggttgcg tgcggaaccc tcgatcctgc ccgacgaggt tcagaccaaa
     5941 cagaagatcg aacagctcaa ggagcgaatc gacgacaagt tgttcagtcg cctcggctgg
     6001 cagccgcgct gcaagccgac cacgctgcct tccggccgaa tcctcttgcc cctctattcg
     6061 gacacttact ccgccgggat catggcgatc agcgatgaca agggccggac ctggttcgcc
     6121 agcgagccgt tggcgggctt cggcagcatt cagccgacgg tactgcgacg atcgagcggg
     6181 acgctggtcg cctacatgcg tgaaaacggc ccattaaagc gaatccgcgt cgcggagtcc
     6241 gccgacgagg gcatcacctg gggtccggtg ggcgtcaccg atttgcccaa tcccgggtcg
     6301 ggaattgacg gcctgcggtt ggccaacggc aattgggtgc tggtctataa cgacaccacc
     6361 gagggccgca gcagtctggc ggtctcgctt tcctccgatg agggacgttc ctggaaatgg
     6421 acccggcatc tggaacgtca caagaccggc agctaccatt acccggccgt gatccaggcc
     6481 gccgacggaa ggatccacgt ggtgtacagc ttcttcaccg acggcggcaa gagcatgaag
     6541 cacgccgcgt tcgacgaggc atggatttcc gagcaggact gaggacattc cctggaacag
     6601 cgtgaaacag gctcgaagaa atgtctcgat gagcgaatcg ccggccttgc gactctgacg
     6661 gtcgcggata ctcatccggc cacggacaat gattgcagac gtgacacttc ggtttatgga
     6721 tgtgggcacc attgtcgtct acctgctcgg gatggcggca ataggcgttt acttcgcccg
     6781 gaaaaagaag accacagagg ggtattttgt cgggaatcgg gcgttccccg gatgggcggt
     6841 cgggctgtcg atgttcgcaa cctccatcag ctcggtgacg ttcctggcat ttcccggtgc
     6901 ggcattcatc ctcgattggc gacagatcgt gcagaacctg acgctcccat tgatcgcggt
     6961 ggtcgccatc ctgttattca tcccgttttt ccggcgcggc aaggtcatgt ccgcttacga
     7021 gtacctggag gaacgttttg gcccgctgct gcggttgtac ggggcgatca acttcattat
     7081 tctgcagttc gtgagactgg gcaccgtcct ttatctcgtc tccatcccgg tgcagattct
     7141 taccggcaca tcgattatct gggtgatcgt gatcgtcgga gttgtggtgt ctttctacac
     7201 catcctcggc gggctggagg cggtcatctg gatcgacgtg gttcaggcga tcgtgctcat
     7261 cacgggtgcc gtacttccgc tgtttttcat cgtggaccag ttgcccggcg gtttggcgca
     7321 gatcgtgcaa gatgggatga aacagcataa gttcagcttg ggccccatga gctgggacct
     7381 ggccgagcgt acgttccccg ccatggtgct tatcggcctg ctgaactgga ccacggagta
     7441 caccaccaat cagaacgtga tccagcggta tctggccgcc tcgtccatgc gtgaagcccg
     7501 caaggcgacc gcgatctgcg cggcgatgag tctgccgatc tgggtcttct tcttcttcgt
     7561 tgggacgagt ctattcgtct attatcaggt gtttcctgat tccggcatcg agcagatcgt
     7621 ttcagagagg agaacggact acatcttccc ttacttcatc ctcagccgga tgccggccgg
     7681 gatcgccggg ctggtcatcg cgggcctctt ggcggcgacc atgtcgcttg attcaagtat
     7741 caattccgtg gcgacggtaa ccacggtgga catcgtcaag cgccacatct gccccggccg
     7801 cgatgatgct ttctaccttc gcgtaggcaa gatcgcgtcg gtgggcgcgg cggttgccat
     7861 gattgtcggg gccgtcgtca tcgctcacct gcccaaggaa agcatggtgg acttgaacct
     7921 cagaattatc cagatcgtgt tcggcggcgg attaatgggg ctcttcctgc tcgggtttct
     7981 caccacgcgg gccaactaca tctccgcctc ggtcgcattg gtgctgacgc ttctgctcga
     8041 cgcctatctt gccctgtgca gcttcgacca actgccggac tgcatttccg ttcgagtcca
     8101 cacatattgg atcggcgtca tcgcgaacgg gtttgttatc ttcgtgggat atgccatgag
     8161 tttcatcacg cccggcgtgc cgaaaccgct tggtcggctg acgatctgga caatgggttc
     8221 accataacac gattgcgagc cgccaggttc ttgcgtcccg gtgaagaatc atcgagccag
     8281 agacgtcgcc atcagccccg cggacgagca tctcaatcgc attgaccgga gcttttcaga
     8341 ctcgcggccg aaggccgtct tattcgtcag gtcagcaggt tgatcaggtc ggcagcccgc
     8401 cacggctgtt gcaggaacag cgtcacgccc agacaggcaa aggcttcgcg tcggtcatcg
     8461 atgctgctac cgatcaggat gacgtcgggc ctgaccgctc gcatttgttc gatcgcgtcg
     8521 gacgggtcgg gtccggccgt gttgtagtcg agcacggcaa actggagtcc ctcgtcggtg
     8581 ccaagcagtc ggacggcctg gtcaaccgac tcgaccgcat agcacccggc gccgacgttc
     8641 tcgatggtcg taacgagcca tttgcgcgtg gttgcgtcgg cctcgatgat gacaacgcgc
     8701 ccatcgaacg gccactgtgt gccccgcggc gagacgacgc cgaggagcat ttcgatcagg
     8761 ggaagcccga catattgcgg accatcgccg cgccggagga tcacaagccg gctgccgtgc
     8821 tcgtccggtt cctgggtacg gaggtgccag gcgattctgc gatcggccac ggggtgagcg
     8881 accttccagt catcgagggc ctggtcgagg tccggcgcag ctccggccgg gaagagctca
     8941 gagaagttgc gagcgccccg ccccttctcg gtctcgtcaa agagatcgag ggcggcgtcg
     9001 ttggcgtggc gaatcccgcc accggttgtg atctcgacga ctgccagcgt tgcccgccgg
     9061 ccggtctgat acggccccgt gagagcgttc tcaaccgcct tgcggatttg ctccaacgtg
     9121 aaaggcttct cgatgaattg ggcgatgccg cttgagtcga cgttctgacg aagctcggtc
     9181 gaggcaaagc cggtgatcag gatggccctg agatccgtca gaaccgcccg caggacctga
     9241 atgacatgca ggccgtgaat gtcgtccttc agcatccagt cagtcaccag caggtcgggc
     9301 cgaaaacgcg cccccacgtc cacgccttct cgtccattcg atgcgcagcg tatctcatgg
     9361 cccagggcgg acaggccgat atccagttgc ctgcgaaagc cttcctcatc ttcaacaatc
     9421 aaaatcctgg ccatgggagc ccccgaatgc cgccgtccgc cgccgtcttg ggcaggttcc
     9481 ccacgggccg gcggcgatgc ccgcccaggc agtgagagtc gcgaaccgct agaatcatga
     9541 tcgcaagggc aggtcgatcg taaaggtcgt gccgccgccg ggccggctgt tcacggagat
     9601 ctgcccgtcg tggtccacaa tgatagcatg cgccagactc aagccaagcc ctgtcccgcc
     9661 acgttctcgt cgggtggtga aaaaggggtc gaaaacccta ggcaaatgat ctcttgggat
     9721 tcccgggccg ttgtcgctca ccgtgactcg gagtcggtcg tcggccacat cggcccgcac
     9781 ctccagcttg acacccttac ctcccgcttc caacgcattc ctcagcagat tgacgaagac
     9841 ctgctccatt tgaagagcat tgagtgagat ttgcgggagg tccgcgccca gatgaacact
     9901 gacagttgct tcctgtcgct gtgcctgggg agcgatcatc tcgaccgacc tgcgaatgac
     9961 ggcttcgatg gatgtcgggg tcttgggggc ctgttcccgg cgagcaaact gaagcacgcc
    10021 ctggatcacc gctccgcagc ggcgggcttc ggagacgatt cctctgaggc agtcatcgac
    10081 cacggcctcg gacccgggca ttttgcggag ttcgagggcg ttctcggccg caagcagcat
    10141 cgtgcctacg gggttgttga tttcgtgggc gataccggcc gcaaacgtac cgagggaggc
    10201 aaggcgttcc gagcggcgga gttgctcttt agactcatcg agctggcggg tccgctcagc
    10261 gacgcgtgcc tcgagattct cgcgataacg ggccaattcg tcctgggcgc gcttctgctc
    10321 ggtgatgtca gccacgaacc cctcaaggaa catcaattcc ccctcggccg agaacacccc
    10381 gcgtcctcgc tcccagaccc atttctcatc gccgccggcg gttcttattc tgtaggtcag
    10441 ttcgaactcg gttccttcgg ccagcgcggc ctgcaccttg tcccaaacct gcaaccgatc
    10501 ctcggggtga atcacttccg cgtaggacgt tttggcgttt tcgagcagat cggccggcag
    10561 gtagccggtc aaggagacac agccctcgct gacgaatacc atggtccagt tgcggtcgtt
    10621 gcgacaccga tacaccatac ccggcagatt ggccagcagt atggacaaga accgctcgtt
    10681 ctctttgagg ctgcttgcga ttctctcgcg ttcgccgatc tctcgaacga ggcgttcatt
    10741 gacctccatc aggccttgcg tgtagtccct tgtcctgcgg aaatggcctg cgacaagccc
    10801 ggttatggcc aagccgagaa tcaaaaggat ccactgagta acgcgttgac gtgcggcctc
    10861 aaaggcgggc gtggatagac aggtgacaag ccattgtcga tcgccgattt gaacggaccg
    10921 gctttggctg cacgaagttg aaagtgtttc gcgaaaagcc gacggggcaa cctcgcgcac
    10981 cgattctccg ggggcttcgt acaaccagca cgacaaggat gttcccggtt caggcgagtc
    11041 ggcaatccgg acgatgacac cgcgaggtcc tgaaggccgc agggcccgat taagataatc
    11101 ctggactcgc agaacaccga tcaccagtcc cgtcaagctc tgccggtgcg attcggggag
    11161 atcgctccgg ccaggcgtgt gaaagaccgg gacaatgacc aggaacgagg gatcatcgga
    11221 gctttgcggc aggtgtgagc cacgggacag gacaatgacc tcacccgcgt cgcatgcctt
    11281 ccggaacagg gccagcttgt cagcatccgt tgacagatcg agcccgagaa acgaaggaac
    11341 gcggttttcg gggtattcgt agtagatcgg gaagtactca ggccggtcag ccgcgacaat
    11401 gagttgtccc gccgcgtcga gatccttgaa ctcgaaatcc tgacggccct ccgcgcgagc
    11461 ccggtcttga taggcggcgc gttccgcctg ccggacgtag ggaacccatt gcagcaacaa
    11521 gccttggccc agcccttgag tcaacctgga aacatacgtc cgaaactcga ccggcgtgac
    11581 atgttccgag ctcgcgaagt aagtggcgac cagtcgtacc tggccgacgc agtgcgagaa
    11641 ttcgcgttcg acatcagagg ctcgatctcc cgccagttcc gcaagttccc cctccatcca
    11701 ctgccgttcc cacgatctgg tcatcaggaa cagacttacg gacacgagag aaccgatgac
    11761 aacggacaac gcgattgcca gggagcctgt cctgattctg ccggacggag caggatgtgc
    11821 agccgtgtgc attcgattgg gcctcgccga atgactgccg cgtggtgacg atgtcgatca
    11881 gacatcatca taacgagggg cgtcgtggaa tcccagccgc caagggcgtc cgggtgtgta
    11941 cgagcctcac cgacccagtc cgccacgccg cggaagcgtc ggtttcgtac cagacgggat
    12001 ggccggttgc gctttgcccg aaccgtcagg caccctacaa tggtcagtcg gcaagagtaa
    12061 ttctcgggaa agtctgggac ggcctttcgg cccatgatta aaccggcagg atggccgtcc
    12121 cttaccacgc gggcaagaag agagatcgca tgacgtcgga cgatttcagc atccttatcg
    12181 gtggagaagc gggacaaggc ttggtcaccg cgggcaccat tttggcgaag gccttgatgc
    12241 gcagcggcct tggggtgttg gtaacccaga gttatgagtc gcgaattcgc ggcgggcaca
    12301 acacgttcgc aatccgttgc gctccgcgtc cggtgattgc cccgagagaa actgtggatc
    12361 tgctcctggc gctgaccggc gacacggtcg ggattcatca aggacagatg acggcgcgag
    12421 gtcgcatgat tgtcgatacg gcgcacgggg cggccgagtc tgattcgtgc gttgccgttc
    12481 cgttcaagga gctggccgac aaacggatgt tcacgatggc cgccacaggg gttgctgccg
    12541 ctttgttggg tctggagcgg acgctgatgg ggcaaacggt gaacgacgtt ctggggacaa
    12601 agcaccctga actgaccgaa gccaaccaga aagtgctgac cgatgcatac gaatgggttg
    12661 ggcagaacgg ctttcccaaa cttggtccgc cgtcggcgcg caaccgctcg cgacggatgc
    12721 tgatggacgg aaacgaggcg cttgcggcgg gagcgatatc ggccggggtt cgattctgct
    12781 cgtactaccc catgactccc ggcagttcga tcatgcaagc aatgatcact cacgccaagc
    12841 atgtgggcgt tatggtcgag caggcggaag atgagatcgc cgcaatcaac atggcgatcg
    12901 gagcttcgta tgcgggggcg ccaagcatgg tgtccacttc gggcgggggt tttgcgttga
    12961 tggtcgaggg cgtgagcctg gcggcgatga cggagacacc cgtcgttgtg gttgtcgcgc
    13021 aacgacccgg tcccgcgacc gggctaccca cgcgaaccga gcaggccgat ctggaattcg
    13081 tgcttcattc cggtcatggc gagttcccac gggccctatt tgctccgggt gacgtcgagg
    13141 agtgtttcca tctgacgcgg aaggcctttg agttgtccga gaagtaccag ggtcccatgt
    13201 tcgtactgac cgaccagttt ctggcggact cgttgcgatc cgccgagcca ttcgacgccg
    13261 ccaaccttca gactgttgcc ccgctcatcg cgtcggccga cgtgcccggt ccctacaagc
    13321 gatacgcgat aaccgacgcg ggcgtctcgc ctcgactggt tccttgtgag acccctcact
    13381 tggtgatcgc cgatagtgat gagcacgatg aggaagggca tctgaccgag gatttgggta
    13441 ttcgggttcg gatggttgac aagcggctca ggaagggacg cggcatctgc ggtgaggttg
    13501 tgcggccggg tcacaccggt gacgattcgc ccgacctgct gctggtcggc tggggatcca
    13561 caaagggctc gatggaagag gctgcggcgg ttcttcgcgg cagggggcag agcgtcggca
    13621 cgctgcattt ctcacaggtt tggccgctgg ttggcgactt gttcgttcct cagttggaga
    13681 aggccggaca ggtcgtgtgt atcgagagca atgcgacggc ccagttcgcc cggctgatcc
    13741 gccgagagac cggattccat atcagccggc gcattcttcg atacgacggg ctgccgatca
    13801 cccccgagta cattcttcgc gagcttgagg cattggagtg cacgtgatgg ttcggattga
    13861 ggattatggc gagttcgaga ccgcgtggtg cccgggatgc ggcaacttcg gcattctgaa
    13921 ggcggtgaag caggctttgg tgcgcgaaaa cgtggcccca cacgaggtgg ttttcgtatc
    13981 gggtatcggg caggcggcca aggcacccca ctatctgaac tgcaatgtat tcaacggtct
    14041 tcacggtcgg gcccttgccg ccgcgacagg tatcaagcta gccaagccca gttgcagggt
    14101 tatcgttgag agcggcgacg gctgcacgta cggcgagggc ggaaaccact ttctggcggc
    14161 gattcgccga aacgtcgatt tgaccgtgct ggtgcacaca aaccaggttt acgggttgac
    14221 caaaggacaa gcgagcccga cctcggatct gggcttcgtc accaagacgc agcctgacgg
    14281 cgtcaaggtc gcgcctttca atcccatcgc gacggccgtt gctctgcggg cgggcttcgt
    14341 ggcccggagc ttcagcggta tgcaggacca cctgaccgag atgatcgtgc aggccatgaa
    14401 ctacccgggc ttcgcgctgg tggacatcct gcaaccctgt gtcactttca acaaggtcaa
    14461 caccttcgcc tggtacaaga agcgatgcca ggaggttcca cccgaccatg atccgacaga
    14521 ctggcaggcg gccatgcggc tggcttacga atggcaggaa cgaatcatga tcggcgtcat
    14581 tttcaagtgc agtcggccgg tgctgacctt cccgcaacac gacggaacac ccaggccgca
    14641 ggcgctgtca gagagcatgc agcgatacta ttgacaaccg attcacccat gcccaaggat
    14701 ccttcggacc acggagcgga tgttctgatc atcggagcgg gcgcggccgg tctggcggca
    14761 gcggtcatgg cggccgaaca gcatcgcgag tcgcgtatcg tgctcttgga aggcacatcg
    14821 aggcctggtt ccaagcttct gctcacgggt ggccggcggt gcaacctcac caaccggacg
    14881 gtgacgccgc aggatttcag cggctcgaac cctcatgcca tcaagaaggt gcttggggct
    14941 ttcaccgtga cgcagacaat cgaattcttc aggagtcttg gcgtcctgct gcacgaagaa
    15001 gaaggcggca agctgttccc gcaacacggc ggcgcccatg ccgtccgaaa cgctttgctc
    15061 ggccgcgtcg aatcgtcggg cttgaagatt cttacgaggc agaaagtcaa acggatcgaa
    15121 caaagctcaa atgcctttgt cgtctcaaca tctgagagca ctttcaccgc gccacgcatt
    15181 ttgctgacga cggggggatg ctcatacccg agtacgggga gcgacggcag cggctatgag
    15241 ttggcgcggt cttgcgggca taccatcatt cccccaacgc ctgccctggt gccactggtg
    15301 ctggccggag ggtttcaccg cggcctcatc gggatcagcc acgacgtcga gctgacgctt
    15361 gtggtcgacg gcaagcgcgt caagacagca ggttcgctgt tgtggacgca ttttggagtc
    15421 agcggaccgg ccgtcctgga catctccggg cactggcttc gaggcaagct ggaagggctc
    15481 agagttgagg tgacggtgaa tctcttgccg ggcgagacgg ccgcctcact cgagcagtgg
    15541 ctgatccggc ttgccagcaa ccatccgaga atgagctttc acagagctct ttcgtcccga
    15601 atgccggctc gcctggccga agcggttttg cacggactcg acgtagatgg aacaacggcg
    15661 atggcgcatc tttccaggga cactcgtcgc cacgttcgaa gcgcgctggt tgcatggccc
    15721 ctgccggttg tggacagtcg cggttacgag cacgccgagg tgacggccgg cggtgtgccc
    15781 ttgaaggagg tcaaccccgc aacgatggag tcccgcatct gcccgggtct gtttctggcc
    15841 ggcgaggtac tggacgtgac cggccgagtg ggcgggttca atcttcaatg ggcctggtcc
    15901 agcgcttggg ttgcagcgag gggcctgacc gtagacaatg ccgcgttgcc gcgggccgag
    15961 gtttcggcgc actcaggacg tcatcactga cgaaccgcac gatgccgcct gtggcggggt
    16021 ttctcggaat cggccatcat gttcatcgag gagacgcaca tgaactcttt tgccagcatt
    16081 ctatggatca cggccggtct gcttcttccc gggcagcccg ccactcaacc tgctgagact
    16141 cgacttgctc gtatcctgat catcaccgga cgtgacgtcc ccgctcacaa ctggcgcgag
    16201 accacgccgg tccttcgcga gcaccttgag cagaccaaga agttcgaggt ggtcgtgtcg
    16261 gaggaaccgt tggtgctcga gtcttcagct cttgatggat acgacgtgat tctgatcaac
    16321 tattacaact ggcagcggcc gagtatcacg aacaaggcga aggaaaacct gctcaagttc
    16381 gtaagagacg gcaagggcct ggtttgttat cacttttcgg tccgggcgtt cggcgactgg
    16441 ccggaatacc gcaacctcgt gggccggatc tggacggacg gctccggtca cgggccgcgc
    16501 gggccgttca aggtcaagat cacggatccg gaccacttca tcacgcaggg gaccagcgat
    16561 tttgatgccg acgacgaact ctatgccaag ctcgtcggcg acgctcccgt tcacgtgctg
    16621 atggaggcct actcagattg gagcaaacgg accgagccgc tggcctggac actggagtac
    16681 ggaaaggggc gagtgttcag catcatgctc ggccacaccg ctaaggactg ccgcaacccg
    16741 gacttcgtgc ggctgttgca gcgcggcact gaatgggtcg cacaaaagga tagtcattga
    16801 gccgggcctt ctgccgcgtg gcattgttgt cgtcaggcat gcgttgaaca tggttgacga
    16861 ccgtcgtcaa acgagaagag gaagatccat gtcaaagcgt atcaccaact gccgcctcgc
    16921 cctgccagcc gttttgtttt tgtggagctc tttcgcctgg gcggaggtca aaccccatgc
    16981 tctgttcagc gacaacatgg ttgtgcagca aggagtgccc atcaacgtgt ggggtacggc
    17041 caaagccggt gagaaagtca ccgtgaagct gagtacacag gaagcttccg ttgttgccga
    17101 tggtgagggc aggtggtcgg tcaggctgga ggcgatgaaa gcaggcgacg ccctgaccat
    17161 gaccatttcc ggggataaca ccatcagctt caagaatatg gcggtgggtg aagtgtgggt
    17221 cgcctccggt cagtcgaaca tgcaatggcc ggtgcgggca tcgaccaatg ccgagaagga
    17281 aatcgccgag gcgaattacc ccaatattcg tctgttcacc gtgtcgcgcg tcgtggccgg
    17341 aaaacctcag accgacgtgc aggggagctg gagcgtctgc agtccccaga ccgttggaga
    17401 cttttccgcc gtcgcgtact tcttcgggcg cgaattgcac aaggcgatca aggttccggt
    17461 tggcctgatc aacacctcgt ggggcggtac tccggctgaa gcatggacca gcatggcggt
    17521 gctggagggc gagccggcct tcaagtcgat cctcgattcg tgggacgcgc gctttaagga
    17581 gtacccggcc gcgatcgagg ctcacatcgc cgcctacggc aaagccgtcc gtgagtggtt
    17641 gaaatcggcc gattccgccg aggccgcagg caaacccatc gccccgccgc caaagctcga
    17701 cttccccggc gaccctcgaa caagcccgca ccgtccggcg ggtttgtaca acgcgatgat
    17761 ccacccgctg ttgcccttcg gcatcaaagg ggccatatgg tatcagggcg aatcgaatgc
    17821 cgggcgagca actcagtacc gcacgctgtt ctcgaccatg atccggaact ggcgcaagga
    17881 ttggggactg ggcgattttc ccttcctgtt cgttcaactg gctccctacc agagggagcc
    17941 ggccaatccg ccaccgggca cttggccgga actccgggaa gcgcaattgc tgacgatgct
    18001 caacgtcccg aacaccgcca tggcggtcat caccgacgtc ggcgaagaaa aagacatcca
    18061 tcccaagctc aagcagccgg tcggcgctcg gctggccctg gccgccaggg cgctcggcta
    18121 tggcgagagg atagaatact ccggcccgat ctacaaagac atgaaacgcg acggcagcaa
    18181 gatcgtcatc agcttcacac acaccggcgg cggcctggtc gccaagggcg ggccgcttaa
    18241 gggcttcacc atcgcgagct ccgatcagaa gttcgtcgac gctcaggccg agatcgtggg
    18301 caatacggtg gtggtctcca gccccgaggt cgccgagccg gcggccgtcc gctacggctg
    18361 ggccaacttt ccggtggtca acctctggaa caaggccgat cttccggcct cgccgttcag
    18421 aaccgatgac tggccgatgg tgacggcagg gaagtagcat cacgccgcaa aatcgtagcg
    18481 gccggtgtcg cggtcggccg tgcagccggt ccttttcatg cccagaatgg ggcgtatctg
    18541 tatggccagg ggcacgagcc cctggtatct cgcatcaact gcccggggcc cattccgcag
    18601 tctcgacctc gcggtgagtt acccaaggaa ctcccgcggg tcggtaatcg tcccgctgac
    18661 cgccgtagcg gcggcggtgt aggggctggc gaggaagacc tgagcgtcct tggagcccat
    18721 tcgtccgggg aaattgcggt tggttgtcga gaggacgcgg atggcctcgt tggcccggcc
    18781 gaaggtgtcg ccggggccgc ccagacaggc ggcacacgag gccggtccca tcgcacagcc
    18841 ggcgttgatg aagatctgct caagcgtcat gccgtcgatc ttctcctcgc gcattttggc
    18901 gtggacctcg gtggtggccg gtacgatgaa agtcggcacc ttgacttcgc gtcccttgat
    18961 gatcttcgcg gccgcccgga aatcagtgat cttgccgccg gtgcaactgc cgatatagac
    19021 acggtcaacc ttggtgccct tgaggttgcg agccaggtcg cgattgtcgg gcgagtgcgg
    19081 cttggcgacg gtcggctcga ggtctttcat cctgaaaacc ttctcgtagc agaacttcgc
    19141 gtccttgtcg ttttcgacgg cttgccaagg tttgtcggtg cgtgcgcgga cgaagtcggc
    19201 ggtgacctca tcgacgggca cgattccgtt ctttccgccg gcctcgatgg ccatgttgca
    19261 caaggtggta cgttcctcga tattaaggcc cattaccccc ccaccggaga actccatggc
    19321 ccggtaggtg gcaccgtcaa caccgatgtc gccaatcacg gtcaagatca tgtccttggc
    19381 catgagatac gacggcaaat cgccgtcgaa gacgaagcgc atggtgggcg gcaccttaag
    19441 ccacagcttg ccggtgccca acacgaagcc ggcgtcggtg ttgccgatgc cggtggcgaa
    19501 ttcgccgaat gccccggccg tgcaggtgtg cgaatcggtt cccagcagca cctcgccggg
    19561 ccgagtatgc ccctcttcag gcagggcaat atggcacaca cccttgtagt ttgtcttggt
    19621 tgggtccttg tacgggctgg gcatgccctc gccctggatg aagtcggggt cgtagtaata
    19681 cttgatgccc tgttccttca cgaaggaccg aaggatatcg atattgcggt gtgccttgct
    19741 gtcggccgtg aagatgtagt ggtcgggaat gatcaccacc ctggacgggt cccacacctt
    19801 ggcgttcctc ccgaaatgct catagaagac gccgattgtg cccggcccgc agacgtcgtg
    19861 tgtcatcagc acgtcgacgt tgacccagac gttctcgccg gcggtcactg aattcctgcc
    19921 ggcgtgtcgg gccaaaatct tctccgtcat cgtcatgccc atcgcgttca cctcgtcaca
    19981 ggccgctgcc gactcgttgc tgccccgaga cccggagcac cggcgaactg acctgccgta
    20041 aatcctctca tggccatgga ttctagcagg ggcggaccct tgagacaatc gcagatcttt
    20101 ctcgggctgc cgcgtcaaca agccgagacc ccagaggttg ctaccctgcg acacatctga
    20161 tgtgcagttt tctctggcga ggaccatcga actcgcagaa gtagattccc tgccacgtac
    20221 caagaacgag actaccgtct tcgaccagga tcgtctgcga ggctcccacc agcgaggcct
    20281 tgatgtgggc atcgctgttg ccctcaccat gccggtagga tgcttccacg tgcggcacca
    20341 gatcagccag tttccagagg aaatcatgaa tcacgtcggg gtcggcattc tcctgaatgg
    20401 tgatgccggc cgtggtgtga ggcacgtagc agatgacgta gccgctgctc agacgagtct
    20461 cacggacagc cttctgcacc ttcgaggtca cgtccacgag ctggcagcgc tcgccggtcg
    20521 tcacggtaat agtctgaatc atcttattcc tccaaggagg tcccgtatcc acagggcatc
    20581 tatgtacacc cgtgccgaca tactatactg aacgagccgt cgaggggaga gccgaggaat
    20641 tgagagttgg agatgactga ccgaaaaggc aaagtcgtcg ttgccatgag cgggggcgtc
    20701 gattcgagtg tcgccgcctg cctgctcctg gagcaaggct acgaggtcat cggccttttc
    20761 atgcggacgg gcgtcaaggc ggttttggat gagccggcgt gcccggcgga tgaagcgaca
    20821 tcgaaccggc cgttccgagg ctgctgcagc gccgcggatg ctgctgacgc ccgtgacgtc
    20881 gccggccggc tgggcattcc cttctacgcc ctggacttca aagagagctt cgaaagcata
    20941 atcgaccact tcgttgccga gtacgttcgc ggccgcacgc ccaatccgtg cattctgtgc
    21001 aatgatcagc tcaagtttgg ccggttggcc gagtacgggc gtgcggcgga cgctgatttc
    21061 attgcaaccg gccactatgc ccggattgtt caacatgccg gtcaagcgcg gttgtgccgg
    21121 ggtgtggaca cggacaagga ccagtcatat gcgctttttc agctcgatcg cgggattctg
    21181 ccacgggtga tgctgccgat cggggagatg accaaggagc gggtccggga ctatgcccgc
    21241 aagcgtgggc tgcccctttt cgataagcgg gagtctcagg acatctgttt cgtacctgat
    21301 cgagactatg ctcggttgat tcgggatcgt cggcccgatg ctttccggcc cggccgtatt
    21361 gtggatcgat cggggcgtga actcggccgg cacgatggaa tcgtgaattt cacgatcggc
    21421 caacggcggg gccttcgctt ggccatggga aagccggtct acgtcacggc catcgacgcg
    21481 gccagcgata cggtgaccat cggcccccgg gaggagcttg cccggagcga actgcgagca
    21541 tccaaggtgc attggctgat cgatccgccg accgagatca tcgaagcgga cgtccaaatt
    21601 cgttataccc acaaggcagc ccgggcggtt gtcgagccga tcggggccga ttgtgtgcgt
    21661 attcgatttc gcgagcctca atacgcgatc acgccggggc aggcagcggt attctacgac
    21721 ggcgacgtcg tcctcggcgg cggctggatt gacctggagt agacagacac ggtgcaatcg
    21781 cctggtgttc ccacgcttgt cttcctgcat cagggcgcga tcggcgattt cgttctggcg
    21841 ttgtcgatcg ttcaagccac agccgccagg gttcgggcgg ctcgcgttgt agcaattgcg
    21901 tccgcgtctt cggcggggct cgcggccggg cggagcatca tcaaccagcg gctgtttccc
    21961 gatcaggtgg gccttcacac actgttcggc gagggggccg ctgcagatcg acggctgcga
    22021 gagcttctgg cggatgcaag ctatatcctc agcttcctcg ggcctccgga ggggccggtc
    22081 cacaatgctc tccgagaggc aacgcaagcc cgggtgatct cgctcgatcc ccgaccgtct
    22141 gcggagactc tcgaccaacg tcgacacatc accgtgcagt gggcaagtga cgtcgccggc
    22201 gcagggttcg agctcaccga actggaaccg cctgctattc gcatggattt gcccgccgaa
    22261 tcgaccgatg acaaccgctc caatgtgatc atacatcccg gcagcggcgg cgatgcgaag
    22321 tgctggccgc tcgatcgctt catggcggta gccgacaagc tgcaggactg ccgcgtcaag
    22381 tggatgctgg ggcctgctga gtgcgagtct gatgcggttt cggtcaagtc gctccgccag
    22441 cgatgcgacg gtcaaaatga ggtcctgctg atcgaggaag acttcgtgaa agctgtaggc
    22501 cacatcgcgg ccgccgatct gtacttgggc aatgacgccg ggatgaccca cgtggccgcg
    22561 gccatgggta tccccacggt cgccatctac ggcccgacgg acccggccgt gtggcgacct
    22621 cttggtaccc acgtctgccc agtggccacg cctcagccgg gcatggccat cgacgcggta
    22681 cccgccaccg ccgtgatcgc ggccgttcac gcccgcttgg cacagctttc atgaagtctc
    22741 atgacaaccc ataagccaag gggtgttaac acatggcacc catgtggcta ctgccagtca
    22801 accgcaatct gcaacgagtg gctggccgag gcggggaagg tcacgtgagt tgggtccgtg
    22861 gagtttgggg ccgtcagggt caggtggtgc gttccgggtt gggctgagat ggtcagcgac
    22921 ggggtggcca ggatcgtctg gctggcgggg agcggttctc tggtcgcgcc gccgatggtg
    22981 gccgtggttg tgaactggcg ggctgaatct tgtgcctcaa tcgcaggctc ggcggccggg
    23041 gtggcgatgg ggccgttgat cgtcggcaag tctgcgtcct tccggccaag gatgtggggg
    23101 ggcggggagg ttgggcgagg tgttcggggt ggatcggatg acgccgcgga cagccggccc
    23161 ccgccgcctg gctgctcgcc cgggttgggc gaggtgttcg gggtggaacg gatgacggga
    23221 aactggccac ggttttc
//
LOCUS       DAOTPW010000067        23175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2274_length_23175_cov_6.105061, whole genome shotgun sequence.
ACCESSION   DAOTPW010000067 DAOTPW010000000
VERSION     DAOTPW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23175
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2274_length_23175_cov_6.105061"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            706..788
                     /locus_tag="P5316_08005"
     tRNA            706..788
                     /locus_tag="P5316_08005"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:740..742,aa:Tyr,seq:gta)
     gene            1128..1871
                     /locus_tag="P5316_08010"
     CDS             1128..1871
                     /locus_tag="P5316_08010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR84938.1"
                     /translation="MDYIRVILFDWGGTLAHVRTQAQAFALGAAKAGQILCGSVGKAE
                     VEQLGRAIISAEAEAAADPSHREADLTRILAEWAAQINPRFTRDQLDAARDALGEQWI
                     GSLEVVPGALEAVRQLREQGLRMGLVSNCSVPPEYCRRELARQGLASLMDFALFSSEV
                     GYRKPSQIIYQEALQRSFADGEEADPSRVLFVGDSPALDVIAPAAIGMKTALVASAPG
                     LWPDEDHARAKPDLRIKNVTELPVLLNGR"
     gene            2003..3574
                     /locus_tag="P5316_08015"
     CDS             2003..3574
                     /locus_tag="P5316_08015"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRR84939.1"
                     /translation="MSAKCLYAVIIICAAMASGLRGEAKPGRPNIVIIMADDMGFSDL
                     GCYGSEITTQNVDRLAAGGLRFTQFYNTARCCPTRASLLTGLYPHQAGIGHMVGQGPT
                     PAYQGYLNDRCVTIAEVLKQAGYRTLMAGKWHVGEDRPHWPCDRGFDEYFGLISGASS
                     YFSIDPGRKFARGNEQIDPVKDRFYMTDAFTDEALSFLEKHGRRPQPFFLYLAYTSPH
                     WPLHAWPGDIAKYRGKYRIGWDALRRQRHKRMIEMGIVDPRWPLSPRDPEAPAWTDAA
                     DKDDLDLRMAVYAAQIDRMDQNVGRVLNKIGEIGADQNTLVFFLADNGACAEKVDRGR
                     KGAAADSKDSYLSYGLPWANASNTPFRLYKHWVHEGGISTPLIACWPAVIDKRGAITR
                     EVGHLIDLMATCLDAAGVEYPATYHDKPIAPLEGKSLIPIFRTGTRQGHEALFWEHEG
                     NRAVREGKWKLVSRHPGEWELYDLEADRTELNNLADKHPDRVKAMVDKYKAWADRCGV
                     LRWDQVTGGKKPGTK"
     gene            3665..5773
                     /locus_tag="P5316_08020"
     CDS             3665..5773
                     /locus_tag="P5316_08020"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR84940.1"
                     /translation="MSLITILAMLTMSVPLNAAKDTRALASFETADDLKALRTHETAI
                     QPTTRHATDGSTALEATFKPAEWPCVKMVPPAPWDWSGFDGLAADMTNPGPTSIDFGL
                     RADDDLTGGPQHWRQGSGRLEPGESATFVLPLGPDPMTYGMRGLPTPSGMRGLRGDQS
                     KPINLSHVVAFQIFMHKPASPMVLILDNIRLVHWNQPLDGIVDQFGQYANADWPGKLK
                     NVSEFAKRLASERKELKSRRALPDRDRFGGWDRRPKLKASGFFRTEKVRNKWWLVTPE
                     GRLFWSIGMDCVGTEASTITTGRENMFAWLPAQDDPLARHAGRVRNVHMGPVKEGATY
                     NFYTANLERKFGPNYFSLWAELTLERLESWGFNTIANWSDHRLYGNGRVPYVATVHIG
                     GNHGRLSSGSDYWGKMHDPYDPQFAVDAANAIRPIAERVRNDPWCLGYFVDNELSWGG
                     FGDEAGRCGLARGALAESSESHAKKAFLSQLRDKYGAVDKLNEAWGIKLADWAALDQP
                     YRHQGNETNAMTADMRAFVKDLARRYFTIVRDEMRKLDPNHLYMGCRFAWQTDEAVQA
                     STETCDVVSFNIYAVTPEPKRMAAVEPFDKPCIIGEFHFGALDRGMFHTGLVAAADQK
                     TRAQMYRDYVHHALDNPLVVGCHWFQYLDQPLTGRSYDGENYNIGFVTVTDSPYPEMV
                     EAARAVHTEAYARRWGKAAQ"
     gene            5863..6870
                     /locus_tag="P5316_08025"
     CDS             5863..6870
                     /locus_tag="P5316_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR84941.1"
                     /translation="MSPRQAVQAAFRFEQVEPVPYWLQWDKELDAGLDEYFGSCAWRK
                     RVVPYVVGRHFGHREKPVSVERSHDAFGTVFRIGNILHVEQPVLSEPSLKGYRWPEPN
                     ELIDWDEIDAFLAPFPQSFRMCGLAMGLFERSWLMRGFENILVDMMLVPDFVHELLDG
                     ILDLHLRVMDRIVARVPLDAYFGGDDWCDQRTVMMGLDLWRQFFKPRLQQMVRHCHEL
                     GLAYVLHSCGNVAPLVDDLLEIGLDGLESLQAESMNVYKLKRKVAGRMVLIGGMGVQR
                     LIPFGTPDEIREETCRLIRELGQGGGYVLGPSKPIPAGTPVANAAAFIETAMQYGASR
                     P"
     gene            7001..7558
                     /locus_tag="P5316_08030"
     CDS             7001..7558
                     /locus_tag="P5316_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRR84942.1"
                     /translation="MTRDQAWTLLNQYTKSDSLIKHALAVEAAMAYYAERFGEDVETW
                     RVVGLLHDFDYERWPRQPEHTREGARILRERGVDEEIVGAILSHAESNRADYPLDRPI
                     RKVLTAVDELCGFITAVAYVRPERLTGMTPKSVRKKLKQPSFAAAVSREDIARGAELV
                     GLDLDEHMSNVIAAMQRASKELGLA"
     gene            7618..8985
                     /locus_tag="P5316_08035"
     CDS             7618..8985
                     /locus_tag="P5316_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR84943.1"
                     /translation="MIRAFHWLMTAALFCLASAAIGAERKPNIVFILADDLGWTDLGC
                     MGSKYYETPHIDRLASQGTKFLNHHHCQNCTPTRAALMSGQYPPRTGVYTVGSLERGK
                     AEHRRMAVPQNQTDLPLDRRTIADQLKAAGYATAMFGKWHIGQQGQYHPAQRGFDEAI
                     VSMGRHFNFVTHPPVTYPPGTYLADFLTDKAVDFITRHKDKPFFLYLPHFAVHTPHEA
                     KKDLFARFKDKAPAGGHGDPTYAAMIYSVDESVGRMMARLDELKLADNTIVIFSSDNG
                     GVGGYEEIGGKGITSNAPLRGGKGMLYEGGVRVPFIVRWPGNTRPGTTCNEPTIHVDV
                     YATLLELAGAPKPPQTLDGESMVALFKDPAAKLKRDAIYTHFPGYLEGYGTGKWRTAP
                     VGTIQMGDWKLLEFFETGRLELYNLRDDPGEKNDLAERMPERVRELHARLAEWRKSTN
                     AAMPK"
     gene            complement(9026..9910)
                     /locus_tag="P5316_08040"
     CDS             complement(9026..9910)
                     /locus_tag="P5316_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR84944.1"
                     /translation="MSKKPEPTLFGSSERPAEPKAGPHGVAVREVECRKLLNRCGIDD
                     FSFNCYVGCGHGCGYCYARFMQRFRPHDEEWGRFVDVRVNAVAALTRQLRRLEPGSVF
                     TCSACDGWQPIEAHYRLTRECCRLLVATGFQLNILTKSKLVLRDLDILTGGRVQIGVT
                     ITTPDENWAAIWEPGASTVAERIEILRQAKAAGMCTAVMFGPLLPGISDTDEALARLF
                     AMAAEMGVDRIWTDLLNPRSRVWPAVQQVLRHHCPELYDHYQRVLFHAAFRRHYERRL
                     NARIRKAAQVAGVAERLT"
     gene            complement(10188..12833)
                     /locus_tag="P5316_08045"
     CDS             complement(10188..12833)
                     /locus_tag="P5316_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HRR84945.1"
                     /translation="MSGPNDISRRGFLVGSATAGASLALMQGCSLTTSQDTGDNMLVL
                     PQGNEPPVRLPDLNPARWIWYPSQRCLQNTFVLFRRNVSLVGKPKKATGWISADSRYL
                     LTVNGKRIQWGPSPCDPRWLEADPIDLTGSLQSGENVIGATVLFYGVGEGTWPAGKPG
                     FLFRLEIEYADGRVETVISDENWRAHLCRAWLPGHYKRDFCRTLQEEFDARLYPYGWD
                     TVEFKPDHDWLAAMPLHCSPNDPPVCADYPDYLHGFHGESHACELRARSTALLKEYMV
                     PAVDLSESLWLTWKRSPEEYFEFGVPGAFDVDRSLAFTEGGPGEWHVQLDGRRGAVLT
                     FELAEQVIGWPGFTIEAPAGTVIELLVHEAHEIGGPPLLNTHWNSWGRFVCREGVNRF
                     ETFDFESVRWLQLHIHGATGKVTVGDVHVRRRIFPWPHQVSVQVDEPELQRLIGATVN
                     TINNSCQETCVDGMARERQQYSGDCGHQLHALHLAFGETTLPRRFITTFSQGQAPDGY
                     FLDCWPAYDRLARMMERTVGLAGWGPLIDHGIGFNFDCWYHYLYTGNLKDLDEPYPRL
                     LRFCDYLTTIRDERGLLKVEGLGIPCVWIDHVAYQKQHHKQCALNLYAAAMLQHALPL
                     ICRAFGDRQRAERAEAFGRELLDAAVKTFWSCKHQTFVANLPWLTQEKDVRLCDRSLA
                     TAILFDQCPDGQTDASLRALVDCPKNMGFSYPANACWRLWALGKMGRADVILKDLRER
                     WATMDSVLLNNTLQEDWKVTADSGSQWSHCPVSPLFVLYMSIAGIKPLEPGFGRCEIR
                     PQLADLRELSLTARTVRGPVEFASKGIQGDREITISLPPRMEGELVLPEAERVDLEPI
                     SPPAVGRRRFRLPAGKAVKLHLRQT"
     gene            complement(13109..13588)
                     /locus_tag="P5316_08050"
     CDS             complement(13109..13588)
                     /locus_tag="P5316_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRR84946.1"
                     /translation="MARVYLETSFISACVTDRTDTASLYQRQVSCEWWDTQRSAHDLF
                     VSAEVIIELSGPDFHGRDEALQKIAGIPRLAIGDEVRGLAGLLVQEKVMPAPVAGDAV
                     HVAVATVHGMDYVLSWNVRHLANPNKIEHLQRICRRVGLMPPSIVTPDMLWGSEDES"
     gene            14302..14706
                     /locus_tag="P5316_08055"
     CDS             14302..14706
                     /locus_tag="P5316_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR84947.1"
                     /translation="MRVPTVLVVDDCEEDLDLVEHALGDEEFKTYRARSGPDAIGLMM
                     STRIDMVVLDLDLPEMSGIAVLEVIRYIPRLMRSQVLVMASPEQAHNVQRARELGACG
                     VVSKPLSPEEICLEVRTAFNEATETLVFAGVL"
     gene            complement(14716..14952)
                     /locus_tag="P5316_08060"
     CDS             complement(14716..14952)
                     /locus_tag="P5316_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84948.1"
                     /translation="MIAEKKSKPRDGLSRRGAPNHGQSQKEEEMTVNPALTWFPVLFL
                     AGRSRIAEPGPIPRAAPANVNNQREPPPIVSAII"
     gene            15062..16294
                     /locus_tag="P5316_08065"
     CDS             15062..16294
                     /locus_tag="P5316_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84949.1"
                     /translation="MNHIRAPIILFLLWTAKAGGATASAPAGEPQAAPLPFPIYVASR
                     IDAAITIDGRLTEPAWRQTALGWGMSHGMEPNVLCPDATLFRIGWNNQALLISLACYK
                     RDIQDDLPENIWRPQEPEIIDTQAVPRHDRERGVVPPVNTADVLISRDGRTVTLSFAP
                     PLTDPAQSVKGPPAPPTGSIHDSVGSRPLDLKIEHAYQGGPNDGLWTVEARVTWQQLG
                     FQPPTAGEDWALNVYRDIRFFSNWAFIAWMRDWGKAEYSRYDLVERFGRVLFVADRPD
                     DAIDKIVKETLPRRGPVRVFAADALVLVEPNVQTVRQRYGEQVEVLKAYAEGIRRQRS
                     RIGNDLPYHPFFTEKKPREHLALASRQLARLTWAVNEKPLWDDPACTVALISHAIPEA
                     REGLSAYKKERLYRGLFE"
     gene            16686..20165
                     /locus_tag="P5316_08070"
     CDS             16686..20165
                     /locus_tag="P5316_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84950.1"
                     /translation="MRFGEGLETPHTKWADPWYAGKPKVLFLANGSGAYDVCELARRV
                     PIEVHGFPTLTWFRLGEWYWLWMWLEQSTGSERAAMLERLLDDRFDAIVLANFRLASL
                     NETCQYKILRQVADGTGLVMFYKHDFDPRLLQGTVADAPAQIVNGVPFAGLEFYRNWF
                     MPSRDILTPDRIAPALLQTHTFGQGRVAIVDYNEQSSIFAAAPGAIAPTMEYDYTAEV
                     QYDYHMALAGRCLLWAARREPRLRWVSRFPDGMEIEALAKPQPVRVQAAWSSEHGEQL
                     RSGLTRWLRVRNLWGEILEDREDVIIPDGDRLQWDLALPAMPAGRHFVDCIISSRQGV
                     ENWGSFAVRVRSSLAIQSIQPSKGFYETSEPIKGQIALSAMTPEDREVRLDVHLEDCY
                     GRVFSAQALKVAAGTDRIDFEIPPGQVVSWGARLRASLTVDRQTVHRSEVEIRIRRPS
                     RGEYPIAMWGAISNYGNHIGNLQMKKLGFTAVLSGDPLPQARDDIGWMTFGSGQGAII
                     HSLGDEIPVPKPAKGEKLNEFLKKRYASLDDLNKAWGTSFALWEDVEPAYGAIDGNTG
                     SFVRLHDCLSAGEFMFADNIRTRREAIEKVNPPGVVGPEGSPVGDPELTLAEATFWGP
                     YLTVRDNLLVNALGRPGVLRGNWFGGYVEDRRVPTRNRHVLWLSILGGNNMIEYFTIP
                     GGLLAPDLTLMPFTEEFLPSWHQIRLGLGPQLAQCRPAGNPVALLHSQPSQHIGEAGG
                     DSTDTIKAHEYLLNLLGDAGYSPQYVSTGQVRQGRLSKGDIKVLFLIHAFALSDEEIK
                     AIRQFAQEGGTVIADIPPAWFDDRCRVRPQRAMDDFFGIVQSAPVASMQQRRTLMSTA
                     VSVESRFGPAQLRGRHPEVADAAEGKPASFLGVEHGNGRSMLINGVLWKEGRDNPDTV
                     RTFRNLLEQFANVQPVFHFSYTHPLGKLGTKVYSYQRGDIWIDAILPPEATDPAVPVR
                     IRMIWPQRRHTYDLRRSTPIVADLTESESSALVKPITGRGDRRRDWPEAASAEPALTT
                     QQVIEAQMIDAIELDVERSSPVVVARLPYRVGGVTLEAPASARRGNSLELKVSVHDHD
                     DKPRPGHVIRIRVFGPDGQERSHYGAWLDGDETPQPWLIRFALNDRLGTWNITATDVI
                     SGLSGSKTFELTR"
     gene            complement(20380..21054)
                     /locus_tag="P5316_08075"
     CDS             complement(20380..21054)
                     /locus_tag="P5316_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR84951.1"
                     /translation="MKRTERLFLASGLTVFVWPWIVLGAVPGVETFDTDTAGFVPNTT
                     SSVVVHVGGGGNPDGYIETRKVLTPPVFDIGAMPLDPDFTGDYAAAGIGQISVDLNFK
                     TDNITAGWIRFRPSIVENGWLYPLTNSFPTNVWNTYVVNFDPTWTDLQAQAAGWLTDN
                     DINPAADPSPPFATVLAGVLTTEVRLASEGSTIVGIDNFAIAVPEPGMISLLAAGIIA
                     VLRRRA"
     gene            complement(21661..21819)
                     /locus_tag="P5316_08080"
     CDS             complement(21661..21819)
                     /locus_tag="P5316_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84952.1"
                     /translation="MKIRSLQPAILGSILQLRHLLGAEGFVARLGGCGDAHTGLRGAL
                     GLLRRGCA"
     gene            21848..>23175
                     /locus_tag="P5316_08085"
     CDS             21848..>23175
                     /locus_tag="P5316_08085"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRR84953.1"
                     /translation="MQRMSIDHRTVSCPSAGCVALWIVALAWGAQAIVTQSLLLREAM
                     VLMSGGELVWGILLCAWLAGVAAGAWIGGRLARRTSRAERRLAIALLLLGILACASIW
                     SFREARVACGIGPGELLSLGTMVIVAILLVLPCGLMVGMAFPLASAIADRAQTPGAGI
                     SVNRIYALESAGSLIGGAMFTFWAVEHLTPIQTVLFCTAITSATLAVLLVRAGSRRIA
                     AAVVAGLAGPVVLTAFLAGPVINQFLVERRWRGLSPTCELVAEAESRYQNLAVGRMAE
                     QYTLYCDGQIAASFPDPFTFVPLAHFWMCQHPDPRRVLLLGGAAEGLLTEVLKHPVEH
                     VDCVEPDPRQIDLILPLLPEQDRTALHDPRVTVHHVDGRLFVKKRQARFDLVIARLPE
                     PTAALRARFYTREFFNELRRNMNDQSVLCLTATAGQARLSSESARYLAGLR"
BASE COUNT         4543 a         7403 c         6916 g         4313 t
ORIGIN      
        1 atgcaaaaaa caggtgcggt ccatgaataa tgggtgttgt tcgaggtatg accgggcagg
       61 agtaactcga catcgtccgc ttgtggtaac gagtgggtca ggggcctttg cgaaaagccg
      121 cggatcaggt gcgttgaatg ccccacccgc cgctttcttg ccgtcacgga cgaagtgaga
      181 cgtttctgca tagacacttg caaacgttac cggagacggc tggacggttt cagttgaacg
      241 ccgagttgcc cattcctttc gacggcccgg gggaaaacgg aggttgatct gatctgcgac
      301 gatccttgct gtactctggg ttgaggcggg agtaccgctg ggcttcaatc agctcgcgat
      361 gagcatcgag atgtccttca gacatcccca ttattaagtt cttctctgat ctggtggggc
      421 tcgggaagga cccacatggg ctcggcgaag gccagtctgt catgggcagc tctagctcgc
      481 ccaaggtcga gattgccctt gccgaagtat tgacgtttac cgtcatttat gatttaatag
      541 gcttgatcgg cggaccatga gactccgtcg atggttttgc ctttgtgact gatgaatttt
      601 cgaggtccgg gcacggctgc catcctgaaa atcgcgtgct ctttgtcgat caggaacgaa
      661 gccacagctt tgggcatctc ggaagtcaat ggtcagtagc cttttgggcg tgtgcccgag
      721 tggacaaagg ggacgggctg taaacccgtt ggcttaagcc ttcggtggtt cgaatccacc
      781 cgcgcccatt gctgccgatt atctgtctgt cacatcaatc atcacttgga acgggcggac
      841 tgccttgcgg ctcaccaacg gctgggccag accaacggtt aacagcaagg tgagtcctga
      901 aaacgtcgac aaaccgagaa tctgccttgt tggagaaact aaattctgtt cattggtacg
      961 ttaagccgcc gacttggctg acgggaatca gcaggacttc aactccgtag ctgcggtcgg
     1021 ccgtctgtga acgattgaac ctcacgcctc ctgacggtat caagcatgag tgtttttgcc
     1081 cggatgcggc gggtcgggtt tcaggcggcc gccgagcgag ttgccgaatg gactacatca
     1141 gagtcattct cttcgattgg ggcgggacgc ttgcccatgt ccgaacccag gcacaagcct
     1201 ttgccctcgg tgcggccaag gccggccaga ttctctgcgg atcggttggc aaggcggagg
     1261 tcgaacaact ggggcgagcg attatctcgg ccgaagctga agcggccgcc gatcccagtc
     1321 atcgcgaggc tgatctcacg cggatcttgg ctgaatgggc agcccagatc aacccgcgct
     1381 tcacgcgcga ccagcttgat gccgcccgcg acgccctggg cgaacaatgg atcggctcac
     1441 tggaagtcgt ccccggagcc ctcgaagccg ttcgtcaact ccgcgagcag ggcttgcgga
     1501 tgggcttggt cagcaactgc agcgtacccc cggaatactg ccgaagagag cttgcccggc
     1561 aaggtcttgc ctcgctcatg gattttgccc ttttctccag cgaggttggc tatcgcaagc
     1621 cctcgcagat catctaccaa gaagcgctcc agaggtcctt cgccgacggt gaagaagctg
     1681 acccatcgcg cgtgctattc gtgggtgatt cgcccgcgct ggacgtgatc gccccggccg
     1741 caataggcat gaaaaccgcc ttggtcgcca gcgcgccggg gctctggccc gacgaggatc
     1801 atgcccgggc caagcccgat ctacgcatca agaacgtgac cgaactgccg gtgctgctca
     1861 acggccgctg agtatccctg tctttccttc ggttcatccc actcagcgcc gcccccccct
     1921 gttttcagag accgtcgggt tgcccaaagg ccgttcatgc cttaccttgc cgggaccaga
     1981 gacccgtcga ggagaaaatg acatgtctgc caaatgcttg tacgcggtaa tcatcatctg
     2041 tgccgcaatg gcatcgggcc tgcggggcga agcaaagccc gggcgtccta acatcgtcat
     2101 catcatggcc gacgacatgg gcttttctga cctcggctgc tacggatcgg agattacaac
     2161 ccaaaatgtc gaccgtctcg cggccggtgg cctgcgtttc acccagttct acaacaccgc
     2221 aaggtgctgc ccgacgcggg cgtctctgct gacaggcctt tacccgcacc aggccggtat
     2281 cggacacatg gtcggccaag ggcccacccc cgcgtatcag ggatacctca atgaccgctg
     2341 cgtcactatt gccgaggttc tcaagcaggc gggctaccgc acgttgatgg ccggtaagtg
     2401 gcacgtggga gaggaccgac cgcactggcc gtgcgatcgc ggattcgacg agtacttcgg
     2461 cctgatcagc ggagcgtcga gctacttcag cattgatccc ggccgaaaat tcgcccgcgg
     2521 caacgagcaa atcgatcctg tgaaagatcg cttctacatg accgatgcct tcaccgatga
     2581 ggcgctttcg ttcctggaga agcacggccg ccggccgcaa ccgttcttcc tgtacctcgc
     2641 gtacacctcg ccgcattggc cgctccatgc ctggccgggg gacatcgcca agtaccgcgg
     2701 aaaatacaga atcggttggg atgcccttcg ccggcagcgt cacaagcgca tgattgaaat
     2761 gggcatcgtc gatccccgct ggccgctgtc tccccgagat cccgaggccc ccgcatggac
     2821 cgacgccgcc gacaaggacg atcttgatct gcgcatggcc gtctacgcgg cccagatcga
     2881 ccgcatggac cagaacgtcg gcagagtgtt gaacaagatc ggcgaaatcg gcgcggatca
     2941 aaacacgctg gtctttttcc tggccgacaa tggcgcctgt gccgagaagg tggatcgcgg
     3001 ccggaaaggc gcagcggccg acagcaagga ttcttacttg agctacggtt taccgtgggc
     3061 caatgccagc aacacgccgt tccggcttta taagcattgg gtgcatgaag gtggtatctc
     3121 cacgccgctc attgcctgct ggccggctgt gatcgataag cgcggagcga tcacgcgcga
     3181 ggtcggtcat ctcatcgatt tgatggccac gtgtctcgat gcggccggcg tggaataccc
     3241 tgccacctat cacgacaaac cgatcgcgcc gctcgagggc aagagtctga tacccatctt
     3301 ccgcaccggc acccgccagg ggcatgaggc gctgttctgg gaacacgaag gcaaccgcgc
     3361 cgtccgcgag ggcaagtgga agctcgtgtc gcgccatccg ggcgagtggg agttgtacga
     3421 tctggaggcc gatcggaccg agctgaacaa cctggccgac aagcacccgg accgcgtcaa
     3481 ggccatggtc gacaagtaca aggcatgggc cgatcggtgc ggcgtgctgc ggtgggatca
     3541 ggtcaccggc ggtaagaagc cgggaaccaa gtgacagagg cctgcatgcg gaagattcag
     3601 agaacgacgg accggtctgc gatctgtcga gcagtcctcc tcggccaact gtggagagca
     3661 acttatgagc ctgataacga ttctcgcaat gctcacgatg agtgttcccc tcaacgcagc
     3721 aaaggatacc cgcgcgctcg cgtctttcga aacagccgac gacctgaaag ccctccgcac
     3781 ccacgaaacc gctattcagc ccacgactcg ccatgccacg gacggttcga ccgccctgga
     3841 agcgaccttc aagccggccg agtggccgtg cgtcaagatg gtcccaccgg caccgtggga
     3901 ttggagcggc ttcgacggct tggcggcgga tatgaccaac cccgggccca cctcgatcga
     3961 tttcggcctc cgggcggatg atgacctgac cggtgggccg cagcactggc ggcagggctc
     4021 gggccgcctg gaaccgggcg aatccgctac gtttgtcctc ccgctgggtc cggatccgat
     4081 gacatacggg atgcggggcc tgccgacgcc gtcgggtatg cgaggtctgc ggggagatca
     4141 gagcaagccc atcaatctct cgcacgtcgt ggccttccag atcttcatgc acaaacctgc
     4201 ttcacccatg gtgctgatcc tcgataacat ccgcctggtg cactggaacc aaccgcttga
     4261 tggcatcgtc gatcagttcg gccaatacgc aaacgctgat tggccgggaa aactcaagaa
     4321 cgtatccgag ttcgctaaac gcctggcctc ggaacgcaaa gaactgaaat cccgccgagc
     4381 attgcctgat cgcgatcgtt tcggcggctg ggacagaagg ccgaagctca aggcctcggg
     4441 cttcttccgt acggagaagg tcaggaacaa atggtggctg gtcacacccg aagggcgcct
     4501 cttctggtcg atcgggatgg actgcgtcgg taccgaggcg tccacgatca ccaccggtcg
     4561 cgaaaacatg ttcgcctggt tgccggcaca ggatgatccg ttggctcgcc atgccggccg
     4621 cgtccggaat gtgcacatgg gcccggtcaa ggaaggcgcg acttacaact tctacacagc
     4681 caatctggag cgcaagttcg gcccgaacta cttctcgctt tgggcggagc tgaccctcga
     4741 gcggctggaa tcctggggtt ttaacacgat cgccaactgg tcggaccacc ggctttacgg
     4801 caatggccgc gtcccttacg tcgccacggt tcacatcggc ggcaaccacg ggcggctgag
     4861 cagcggttca gactattggg gcaaaatgca cgatccctac gacccgcagt ttgccgtgga
     4921 cgcggccaat gccatccggc cgattgccga aagagtcaga aatgatccct ggtgcctcgg
     4981 ctatttcgta gacaacgagc tgagttgggg cggtttcggt gacgaagcag ggcgttgcgg
     5041 cttggcacgg ggagcactgg ccgagtcttc tgagtcgcac gccaagaaag ccttcctctc
     5101 gcaactgcgc gacaagtacg gagccgtcga caagctcaac gaggcctggg ggataaagct
     5161 ggccgattgg gcggcgcttg atcagccata caggcaccag ggcaacgaaa caaacgccat
     5221 gaccgccgat atgcgggcct tcgtgaagga cctggcccgt cgatacttca ccatcgtgcg
     5281 cgacgagatg agaaagctcg accccaatca cttgtacatg ggctgcaggt tcgcctggca
     5341 gacggatgaa gcagtgcagg cttccaccga aacctgtgac gtggttagtt tcaacatcta
     5401 cgccgtcacg cccgaaccca aacgaatggc cgccgtcgag ccgtttgaca agccctgcat
     5461 cattggcgaa ttccacttcg gggccctgga tcgcgggatg tttcacaccg gcctggtggc
     5521 cgcagccgac cagaaaaccc gggcccagat gtaccgcgac tacgtacacc acgctctgga
     5581 caaccccctg gtcgtcggtt gccactggtt ccagtacctc gatcagccgc ttaccggccg
     5641 cagctacgac ggcgagaact acaatatcgg ttttgtcacc gtgaccgact cgccgtatcc
     5701 tgagatggtc gaagccgccc gcgccgtaca caccgaggcg tatgcccgac gatggggcaa
     5761 agcggcacaa tgagggtcac gattcaagcg tgacgaggag cggaggcatc acggccgggc
     5821 aagttggagg tctcccgtct ccgtcagagt gaggtttgtt tcatgtcccc tcgccaggcc
     5881 gtgcaagccg cgtttcgctt cgagcaggtt gaaccggtgc cctattggct gcaatgggac
     5941 aaggaattgg atgcggggct cgatgagtat ttcggctctt gcgcctggcg gaagcgagtc
     6001 gtcccgtacg tcgtcgggcg ccatttcggc caccgcgaga agcccgtcag cgtcgagcgc
     6061 agccatgacg ccttcgggac cgtcttccgc atcggtaaca tccttcacgt cgagcaaccc
     6121 gttctgtccg agccgagcct caaaggttac cgctggcccg agcccaacga gctgatcgat
     6181 tgggacgaaa tcgatgcgtt tctcgcgccg ttcccccaaa gctttcgcat gtgcggcctg
     6241 gccatggggc tcttcgagcg aagctggctc atgcgcgggt ttgagaacat cctcgtcgac
     6301 atgatgctgg tccccgattt cgttcatgag ttgctggatg gcattctcga tctgcatctg
     6361 cgagtcatgg acaggatcgt cgctcgtgtg cccctcgacg cctactttgg aggcgacgat
     6421 tggtgcgatc agcgcaccgt gatgatgggc ctggaccttt ggcgacagtt tttcaagccg
     6481 cggctgcaac agatggtccg ccactgccac gaactcggtc tggcttatgt gcttcattcc
     6541 tgcggcaatg tggcgccgct cgtggatgat ctgttggaaa tcggcctcga cggacttgaa
     6601 tcactgcaag ctgaatcgat gaacgtctac aagctcaagc gtaaggtcgc cggccggatg
     6661 gtcctcatcg gcggcatggg cgtacagcgg cttatcccgt tcggcactcc cgacgagatt
     6721 cgcgaggaga cctgccgcct gatccgggaa ctcggacagg gcggcggcta tgttctgggg
     6781 ccgtcgaagc cgattccggc cggcacgccg gtcgccaacg ccgccgcatt cattgaaacg
     6841 gccatgcagt acggtgcatc ccgcccatag aatgggttgg gccaacggcc gtgatcggcg
     6901 gaaaacagct ttcacgcccg aagtggacag ccccaggccg ggtgttattg tgtggattgg
     6961 gaaccggccc cgcagcgacc ttgtcgggag tattaccgag atgacccgcg accaagcctg
     7021 gacgttgctc aaccagtaca ccaagtccga ttcgctcatc aagcatgctc tggccgtcga
     7081 ggccgccatg gcatactacg cagaacggtt cggcgaggat gtcgagacct ggcgggtcgt
     7141 cggtctgctt cacgatttcg actatgagcg ctggccccgg cagcctgagc acacgcgaga
     7201 gggagccagg atcctgcgcg aacgcggcgt cgacgaggag attgtcggcg cgattctctc
     7261 tcatgccgag tccaaccgcg ccgactaccc cctggaccgc cccatccgga aggtgctgac
     7321 ggcggtcgac gagctgtgcg gcttcatcac cgccgtcgcg tatgtccgcc ccgagcgcct
     7381 gaccgggatg acgcccaaga gcgtgcgaaa gaagctcaag cagccgtctt tcgccgccgc
     7441 cgtgagtcgc gaagatatcg ccaggggcgc cgaactggtc ggattggacc tcgacgaaca
     7501 catgagcaac gtcatcgcgg ccatgcaacg cgcgtccaag gagcttggct tagcgtgagg
     7561 gtcggaacag gtaagccgcg gcccttcgag cgctgacaat cgaaaaggag tcccacggtg
     7621 attcgagcat tccattggct gatgacagcc gcgttgttct gtctcgcatc cgctgcgatc
     7681 ggggcggagc gcaagcccaa tatcgtcttc atcctggccg atgatctcgg ctggacggac
     7741 ctcggctgca tggggtccaa gtactacgaa acgccgcaca tcgatcgact cgcctcgcag
     7801 ggcacgaaat tcctcaatca ccatcactgt cagaactgca cgccgacccg ggcagccctg
     7861 atgtccggcc agtacccgcc tcgcaccggt gtatacacgg tcggctctct ggagcgagga
     7921 aaggccgaac atcgaaggat ggccgtaccg caaaaccaga ctgacttgcc cctcgatcgc
     7981 cgcaccatcg ccgaccagct caaagccgcc ggttatgcca ccgcgatgtt cggcaagtgg
     8041 cacatcggtc aacaaggtca ataccatccg gcccagcgcg gcttcgacga agcaatcgtc
     8101 agcatgggcc ggcacttcaa cttcgtcacc cacccacccg tgacctatcc gcccggcaca
     8161 tacctggccg acttcctcac cgacaaggcc gttgatttca ttacccggca caaggacaag
     8221 ccgttctttc tctacctgcc gcacttcgcc gtgcatacgc cgcacgaggc gaaaaaggac
     8281 ttattcgcca ggttcaagga caaggccccc gcaggcggcc acggcgaccc gacatatgcg
     8341 gccatgatct acagcgtcga cgagagcgtc ggtcggatga tggccaggct cgacgagctc
     8401 aaactcgccg acaacacaat agtcatattc tcgtcggaca acggcggagt gggcggctat
     8461 gaggagatcg gcggcaaagg catcacatcg aacgctccgc tccgcggcgg taagggtatg
     8521 ctttacgaag gcggcgtgcg cgtgcccttc atcgtccgct ggccgggaaa taccagaccg
     8581 ggcacaacct gcaacgagcc gaccattcac gtagacgtgt acgcgacgtt gctcgaactg
     8641 gccggcgccc ccaagccgcc gcaaaccctc gacggtgaga gcatggtcgc gctgttcaag
     8701 gatccggccg ctaagctcaa gcgagacgcc atttacacgc atttccctgg atatctggag
     8761 ggctatggca cgggcaagtg gcgcaccgcg cctgtgggca caatacagat gggagactgg
     8821 aaactgctcg agttcttcga gaccggccga ctggaactgt acaacctgcg cgacgatccg
     8881 ggtgagaaaa acgatctcgc ggagaggatg cccgagcgcg ttcgcgaatt gcacgctcgc
     8941 ctggccgaat ggcgaaagtc gaccaacgcc gctatgccca agtaaaaacg gccttgagtc
     9001 gaaaagcccg ccccaatccc ggcgtctacg tcaggcgctc agccactccc gccacttggg
     9061 cggccttgcg gatgcgggca ttgagccggc gctcgtaatg acgtcgaaaa gcagcgtgaa
     9121 ataaaactct ctgatagtgg tcataaagct cgggacagtg gtgacgtaag acctgctgaa
     9181 cggccggcca gacgcgcgaa cgcggattga gcaggtcggt ccagatccgg tccacgccca
     9241 tctcggccgc catcgcaaac aacctcgcca gagcctcgtc ggtgtcgctg atgcccggca
     9301 gcagaggccc gaacatcacc gccgtgcaca tacccgcagc cttggcctgc cgaagaatct
     9361 cgatccgctc ggcaacggtt gacgcccccg gctcccagat agccgcccag ttctcgtccg
     9421 gcgtggtgat ggttacaccg atctgaacac gcccgccggt cagaatgtcg agatcgcgca
     9481 gcaccagctt gctcttggtc aggatgttga gttggaaccc ggtcgcaacg agcaggcggc
     9541 agcactcgcg cgtcaggcga tagtgtgcct cgatcggctg ccacccgtcg cacgcgctgc
     9601 aggtgaacac cgaccccggc tccagccgcc gaagctgccg tgtcaaagcc gcaaccgcgt
     9661 tcacgcggac atcgacgaac cgcccccact cctcgtcgtg cgggcggaat cgttgcatga
     9721 accgcgcata gcagtaaccg cacccatgcc cgcaaccgac gtagcagtta aacgagaagt
     9781 cgtcgatgcc gcagcgattc agcagcttgc ggcactcaac ctcgcgaacg gcgacaccgt
     9841 gcggacctgc cttcggttct gccggccgct ccgaggagcc gaacagtgtc ggttcgggct
     9901 ttttgctcat aacgtgtgtc cccgaaggag gccgtgatag gagctcggcg attcatcgct
     9961 cggaacgaac ggcatcccga agaccattcg ctggcctcag aatccgctat gcatcgctgg
    10021 ccccagaatc cgttatgcat cgctggcccc agaatccgtt ctggggcctt tcgcgtggag
    10081 gattcccatc ctcattctct gctgggctac tctcgtgcgt gcctgcggca ctattctgcc
    10141 ggggggatgc tcccggacga cgttcgtcga accgaccccg gtgaccctca cgtttgtcgc
    10201 agatgaagct tgaccgcctt gcccgctggc agccggaagc gtcgccggcc gacggccggt
    10261 ggcgaaatcg gctccaggtc cacccgttcg gcctcgggca ggaccagctc accctccatc
    10321 cgcggcggca gcgaaatcgt gatctcccgg tcaccttgga tccccttgga agcgaactca
    10381 accggaccgc gaaccgtgcg ggcggtcagg ctcaactcgc gcaggtcggc aagttgcggc
    10441 cggatctcgc aacggccaaa gcccggctcc aacggcttaa ttcccgcgat actcatgtac
    10501 aacacgaaca gcggcgagac cggacaatgg ctccactgcg agcccgagtc cgccgtcacc
    10561 ttccagtcct cctggagcgt gttgttcaac agcacggaat ccatcgttgc ccaccgctcg
    10621 cgcaaatcct tcaggatcac gtccgcccgc cccatcttgc ccagggccca cagccgccag
    10681 caggcgttgg ccggatatga aaagcccatg ttcttcggac aatccaccaa cgcccgcaac
    10741 gacgcgtcgg tctgcccgtc cggacactgg tcaaagagta tggccgtcgc caatgaacga
    10801 tcacacaaac ggacgtcctt ctcctgcgtc agccacggca gatttgccac gaacgtctga
    10861 tgcttgcagc tccagaacgt cttgaccgcg gcgtccagca gttcacgccc gaacgcctcg
    10921 gcccgctccg cccgctgacg atcaccaaac gcccggcaga tcaagggcag cgcatgctgg
    10981 agcatcgctg cggcatagag gttcagcgcg cactgcttgt ggtgctgctt ctgataggcc
    11041 acgtggtcga tccagacaca gggaatgccc agaccttcga ccttgagcaa cccgcgttcg
    11101 tcgcgaatgg ttgtaagata gtcgcagaac cgcaacaacc gcggatacgg ttcgtccaaa
    11161 tccttcaggt tgcccgtgta caggtagtga taccagcagt cgaagttgaa gccgattcca
    11221 tgatcgatca gcggccccca gccggccagg ccgacggtcc gttccatcat ccgggccagg
    11281 cgatcgtatg ccggccagca gtcgaggaag tagccgtcgg gtgcttgccc ctggctgaat
    11341 gtggtgatga aacggcgggg cagcgtggtc tcgccgaacg ccaggtgcag agcatgcaac
    11401 tggtggccgc aatcaccgct gtactgttgc cgctcgcggg ccatgccgtc cacgcacgtc
    11461 tcctggcacg agttgttgat cgtgttgacg gtcgccccga tcagccgctg caactcgggc
    11521 tcgtcaacct gaaccgagac ctgatgcggc cacgggaaga tcctccggcg cacatggacg
    11581 tcgccgacgg tgaccttgcc cgtagcgccg tgaatgtgca gttgcagcca gcgaaccgac
    11641 tcgaagtcga aagtctcaaa gcgattcaca ccctcgcggc agacaaagcg gccccaactg
    11701 ttccagtgcg tgttgagcaa cggcgggccg ccgatttcgt gggcctcgtg cacgagcagt
    11761 tcgatcacgg tgcccgccgg ggcctcgatg gtaaagcccg gccagccgat cacctgttcg
    11821 gccagctcga acgtgagtac cgcgccgcgc cggccgtcaa gctggacgtg ccactcgccg
    11881 ggaccgccct ccgtgaacgc caggctgcgg tccacgtcga acgcgcccgg aacgccgaat
    11941 tcaaagtact cctccggcga ccgtttccag gtcagccaca gcgattcgga gagatccacc
    12001 gcgggcacca tgtattcctt cagcagcgcg gtgcttcgag cgcgcagttc gcaggcgtgg
    12061 gactcgccgt ggaaaccgtg caggtaatcc ggatagtcgg cacagaccgg cggatcgttg
    12121 ggcgaacaat gcaacggcat agccgcgagc caatcatgat cgggcttgaa ctcgaccgtg
    12181 tcccaaccgt agggatacag gcgggcgtcg aactcctctt gcagcgtccg gcagaaatcc
    12241 cgcttgtagt gacccggcag ccatgcccga cacaaatggg cacgccaatt ctcgtccgaa
    12301 atgaccgttt cgacccgacc atcggcgtac tcgatttcca ggcggaacag gaaacccggt
    12361 ttgcccgccg gccaggtccc ttcgccgacg ccgtagaaca ggacggtcgc gccgatcacg
    12421 ttctcgccgc tttgcagcga accggtcaga tcgattggat cggcctccag ccaccgcgga
    12481 tcacacggcg acggccccca ctgaatccgc ttgccattca ccgtcagcag gtaccggctg
    12541 tcggcgctga tccagcccgt cgccttcttc ggcttgccga caaggctgac gttccgccgg
    12601 aacagcacaa acgtgttctg cagacagcgc tgcgacggat accagatcca tcgcgcggga
    12661 ttcaggtcgg gcaaccgaac aggcggctca ttcccctgcg gtagaacaag catgttgtca
    12721 cccgtgtctt gcgaggtcgt gagcgagcag ccctgcatca gcgccaggga agccccggcc
    12781 gtcgccgaac caacgagaaa acctcggcgg gaaatatcgt tcggtccact catgaggcac
    12841 cctcaggtga atgaaaagcc gtcgacgagc agggtggaca cccggtgatc tgccgagggg
    12901 actgtccgac agtcaggacg gggaagaccg cattgacggc ttgcgatatg ccggttcttg
    12961 aataatcgtc cattcaggat gttggcgctc caactcctgc aggcgatcgc acaaccggtc
    13021 aacatcgttt ccggactctt cggaaatgga tcgccgaatt gtacgtacct cgtcgatcaa
    13081 agtgtcgggt tgtcttcgtc cttgtgcgtt aggattcatc ctctgacccc catagcatgt
    13141 ccggtgtgac aattgaaggc ggcatcaaac caacgcgccg acaaatccgc tgtaagtgct
    13201 caatcttatt ggggttcgca aggtggcgga cgttccaact cagcacgtaa tccatgccat
    13261 gcaccgtggc gacggcaacg tgaaccgcat cacctgcgac tggtgcgggc ataaccttct
    13321 cctgcaccag aagccccgcc aatccgcgaa cctcgtcacc aatggccagt cgcgggatcc
    13381 ctgcaatctt ctgaagagcc tcatcgcgac catggaaatc cggaccggat aattcaatga
    13441 tcacctcagc ggacacgaac aaatcgtgag ccgaacgctg agtgtcccac cactcgcaac
    13501 tgacctgccg ctgatagagg ctggccgtat ccgtgcggtc cgtcacgcag gcgctgatga
    13561 agctcgtttc cagatacaca cgtgccatca ccacattata gcggactcgg cagcggccac
    13621 ggaagcgtct ctggctccct cttcatccgc cagaacggga accatcggtc accgaccgcc
    13681 cggcacagtc gaggcgtctc cacaggcttg tttcaccaac cctcgctatt caggcccaca
    13741 tcatcgatcc gcaggtattc cccctgcaga gtcaggtggg aatgccgatc tgcgcaggta
    13801 ggatacccgt caatcacaag cacgcaaagg accgcctgcg gcagggtccg tgatcgttca
    13861 ggcgtttccc cgcctggacc gtcacagacc ctgtgtacct ggccggtgaa agcattatcc
    13921 ggcggtcggt tcgctgagag ccggttccgt aaccgaggtt cgagccgcac gtccgcatat
    13981 ctcaaccgtg tggccgataa gcacggttat ttgctcgtcg cgagcccccg ttttcagata
    14041 gtatttgaca ttcatcgatc tgaattcgat gtttgaggga ctgcttttca gttcgagccg
    14101 gggtggcagc ggtccaggca cgtccgccgg ccggctgatg ccagtccgag tgttcgcggg
    14161 cacaaaccgc cggcttgtcc gattccagag catccgaagc cgggacgccg tcggcttgtt
    14221 gccgatggca ctccgcagat gcgaccggcc gtggggtggg agtaatgggt ccgaccaggc
    14281 ggcgggttgt tgggggaagt gatgcgcgta cccaccgtat tagtggtaga cgattgcgaa
    14341 gaagatctcg acctggttga gcatgcactg ggtgacgagg agtttaagac ctaccgggct
    14401 cgctcgggtc ccgatgcgat cggactgatg atgagcaccc ggatcgatat ggtcgtcttg
    14461 gatctcgatc tgcccgagat gagcggaatc gccgttctgg aggtgatccg ctacatcccc
    14521 cggctgatgc gctcccaggt gctggtcatg gcctcgccgg aacaggccca caacgtccag
    14581 cgggcgcggg aactcggcgc ttgcggggtg gtgagcaagc cgctatcgcc cgaagagatc
    14641 tgtctcgagg tccggacggc attcaacgaa gctactgaaa cgctggtttt cgccggcgtg
    14701 ctgtgagctt ggcgatcaga tgatcgccga aacgatcggt ggcggttccc gctggttgtt
    14761 cacgttcgcc ggcgcagccc tcggaatcgg gccaggctcg gcaatgcgtg accgacctgc
    14821 caaaaacagc acgggaaacc aagtgagcgc gggattgacg gtcatctcct cctccttctg
    14881 cgattgcccg tgattgggtg ccccgcgacg gctcagaccg tcgcggggct tgctcttctt
    14941 ttcggcgatc aaacacccct ccttgccagc cacaacctga atccctagca tgtccgccaa
    15001 cagagatgac cgatgttttc ggtcgcacgc gagggcaacc cacaaccgat acgaggtcgc
    15061 aatgaaccat atacgcgctc cgatcattct gtttctacta tggacagcaa aggcgggggg
    15121 ggcgaccgca tccgccccgg ccggcgagcc gcaggcagca ccattgccat tccccatcta
    15181 cgtcgcctcg cgaatcgacg cagcgatcac gatcgacggc cggctcactg agccggcctg
    15241 gcggcagacg gccttgggct ggggaatgtc gcacgggatg gaaccgaacg tcctgtgtcc
    15301 cgacgccacg ctgtttcgca tcggctggaa caaccaggcc ttgctcattt cgctggcctg
    15361 ctataagcgc gatatccagg acgatttgcc ggagaatatc tggagaccgc aggagccgga
    15421 aatcattgat acacaggcgg tgcctcgaca tgaccgcgaa cgtggcgtcg tcccgccggt
    15481 caacaccgcc gatgtgctca tctcgcgaga tggtcggact gtcaccctca gctttgcccc
    15541 gcccttgacc gaccccgctc agtcggtcaa gggaccaccc gccccgccaa ccgggtccat
    15601 acatgattcc gtcggcagcc gcccgcttga tctgaagatc gaacacgcct accaaggcgg
    15661 cccgaacgac ggcttgtgga cggtcgaagc ccgtgtcacc tggcaacaac tcggcttcca
    15721 accgcccacc gccggcgaag actgggctct gaacgtctat cgcgacatcc gatttttctc
    15781 caactgggcg ttcatcgcct ggatgcgcga ctggggcaag gccgagtact cgcgatacga
    15841 cctcgtcgag cgattcggtc gtgtcctctt cgtggccgac cggccggacg acgccatcga
    15901 caagatcgtc aaagagactc ttccccgacg cgggccggtg cgcgtattcg ccgctgacgc
    15961 cttggtgttg gtcgagccaa acgtgcaaac cgtgcgacag cgatacggcg agcaggtcga
    16021 ggtcctcaaa gcttacgccg aaggcatccg tcgtcagcgg agccgcatcg gcaatgacct
    16081 gccatatcac ccctttttca ccgagaagaa acctcgcgag cacctggcgt tggcgtcgcg
    16141 tcaactggcc cggctgacct gggcggtaaa cgagaagccc ttatgggacg acccggcctg
    16201 cacggtcgca ttgatttcac acgccatccc ggaagcccgc gaaggcttat ccgcctacaa
    16261 gaaggaaagg ttgtaccgtg gcctgttcga gtaaccacct gatcgcggct ctgccggggt
    16321 ttcttgacgt tggccgctcg gcccggaatc cctttcgctc ggcccggagt cccttggtcc
    16381 gcttggcccg gaatcctttc cgggcccgtc ctgcgaaccg ctcggcccgg aatcctttcc
    16441 gggccgtccc gctgcgcagc gtccactcca cgtgcggcgc gaatgaggat ggggatcccc
    16501 gacgcagtag gcccccgaag ggattctggg gccagcggct gatcgcgact ctgcttattg
    16561 ctccggttgt agcatttccc aacccgtgct ccgcaagaga tggggaaccc gccggctcag
    16621 ccttcggtgc gacggccgaa gagcgactcg gacaactcgg ctggcgactc gacggcgatg
    16681 ctctgatgcg cttcggtgag ggcctcgaaa caccgcacac caagtgggcc gacccgtggt
    16741 acgcgggcaa gccgaaggtg ctgttcctgg ccaacgggag cggagcctac gacgtgtgcg
    16801 aactggcccg gcgtgtgccg atcgaggtcc atggctttcc tacgctgact tggttccgcc
    16861 tcggcgaatg gtactggttg tggatgtggc tggaacagtc gaccggcagc gagcgggccg
    16921 caatgcttga acgcctgctc gacgatcgtt tcgacgccat cgtgctcgcc aacttccgcc
    16981 tcgcgtcgct caacgagacc tgccaatata agatccttcg ccaagtggcc gatggcaccg
    17041 gcctggtgat gttctataag cacgatttcg acccgcgcct gcttcagggt accgttgcag
    17101 atgctcctgc tcagatcgtc aacggcgtcc cgttcgcggg cctcgaattc taccgcaact
    17161 ggttcatgcc cagccgcgac atcctcacgc ccgatcggat cgcgcccgcc ttgctgcaaa
    17221 cacatacctt cgggcaaggg cgcgtcgcga tcgtcgacta caacgaacaa tcaagcatct
    17281 ttgccgccgc cccaggtgcg atcgccccga ccatggaata cgactacacc gccgaggtcc
    17341 aatacgatta ccacatggcc ttggccggcc gttgcctgct ctgggcggcc cggcgcgagc
    17401 cgcgacttcg ctgggtcagc agattccccg acggcatgga aatcgaggct ttggccaagc
    17461 cgcaacccgt ccgcgtccag gcggcgtggt cgtccgagca cggcgaacaa ctgcgatcag
    17521 gcctgacccg atggctgcgt gtccgcaacc tctggggcga aatcctcgaa gaccgcgaag
    17581 acgtcatcat tcccgacggt gaccgcctcc aatgggatct tgccttgccc gcgatgcctg
    17641 ccggccggca cttcgtcgac tgcatcatca gcagcaggca aggtgtggag aattgggggt
    17701 cgttcgctgt gcgcgtgcgc tcctcactag ccatacagag catccagcct tccaagggct
    17761 tctacgaaac gagtgagccg atcaaagggc aaatcgcttt gtcggccatg acgcctgagg
    17821 atcgagaggt gagactggac gtgcaccttg aggattgcta cggccgggtg ttctcagccc
    17881 aggctctaaa agtcgcggcc ggtaccgacc gaatcgattt cgagattccc ccaggacagg
    17941 ttgtcagttg gggcgcacgg cttcgcgcga gtctcaccgt cgaccggcaa acggttcacc
    18001 gcagtgaagt cgagattcgc atccgacgcc cgtctcgcgg cgagtatccc atcgccatgt
    18061 ggggtgccat atccaattac ggcaaccaca tcggcaacct ccaaatgaaa aaactcggtt
    18121 tcaccgccgt gctttccggc gaccccctgc ctcaggcccg cgacgacatc ggctggatga
    18181 ccttcggcag cggccaggga gcaatcattc attcgctcgg cgacgagata ccggtgccca
    18241 agcccgcaaa gggcgagaag ttgaatgagt tcctgaaaaa acgctatgcc tcgctcgatg
    18301 atctcaacaa agcgtggggc acgagctttg ccctttggga ggatgtcgaa ccggcctacg
    18361 gcgccatcga cggcaacacc ggcagcttcg tgcggctgca tgattgcctc tcggccggcg
    18421 agttcatgtt cgccgacaac atccgcaccc gacgagaagc catcgagaag gtcaatccgc
    18481 ccggcgtcgt cgggcccgaa ggctctccgg tcggcgaccc tgagctgacc ctggccgagg
    18541 ccacgttttg gggaccatac ctgactgtgc gagacaacct cctggtcaat gccctcggac
    18601 gacccggcgt gctgcgcggc aactggttcg gcggctacgt cgaagaccgc cgcgtcccca
    18661 ctcgcaaccg ccatgtgctc tggctgtcga tcctcggcgg caacaacatg atcgagtact
    18721 tcaccattcc gggtggtctg ctcgcgcctg atttgacgct catgcccttc accgaggaat
    18781 ttcttccctc gtggcaccag atccggctcg gcctcggccc ccaactggcc caatgccgcc
    18841 cggccggaaa ccccgtcgcg ctcctccact cgcagccgtc ccagcacatc ggcgaggccg
    18901 gcggcgattc caccgacacc atcaaggccc acgagtacct gctcaacctc ctcggcgacg
    18961 cgggctactc gccgcagtac gtctccaccg gccaagtccg ccagggacga ctgagcaagg
    19021 gcgatatcaa ggtcctattc cttattcacg cgttcgctct gtctgacgag gagatcaagg
    19081 ccatccggca gtttgctcaa gagggcggca ccgtcatcgc agatatcccg cccgcgtggt
    19141 tcgatgacag gtgccgggtt cggccgcagc gggcgatgga cgatttcttc ggaatcgttc
    19201 aatcggctcc cgtcgcatcg atgcaacagc ggcgcacgct gatgagcacg gccgtctccg
    19261 tcgaaagccg gttcgggccg gcacaactgc ggggccgtca tcctgaagtc gcggatgctg
    19321 ccgagggcaa gcctgcctcc ttcctgggtg tcgagcacgg caacggacgc tccatgctga
    19381 tcaacggcgt gctctggaaa gaaggtcgcg ataatcccga cacggtgcgg actttccgga
    19441 atctgctgga gcagttcgcc aacgttcaac ccgtgttcca tttctcctac actcatcccc
    19501 ttggcaagct cggcaccaag gtctattcct accagcgtgg cgacatctgg atcgacgcca
    19561 tcctgccgcc cgaggctacc gatccggcgg tgcctgtccg gatcaggatg atctggcccc
    19621 agcggcgcca cacatacgac ctgcggcgat cgacgcctat tgtcgctgac ctgacggaat
    19681 ccgaatcctc cgcgcttgtc aagccaatca cggggcgggg cgaccggagg cgagactggc
    19741 cagaggccgc atcggccgag ccggccctga ccacgcagca ggtcattgaa gcccagatga
    19801 ttgacgccat cgagctggac gtggagcgaa gttcgcctgt ggtcgttgct cgtttgccct
    19861 acagagtcgg cggtgtcacc ctggaggcac ccgcttcagc gcgcagaggc aacagtctgg
    19921 agttgaaggt ctccgttcac gaccacgacg acaagccgcg tcccggccac gtgatccgaa
    19981 tccgggtctt cggccctgat ggccaggagc gttcccacta cggcgcctgg ctcgatggcg
    20041 acgagacgcc ccagccgtgg ctgatccggt tcgccctcaa cgaccgcctg ggaacatgga
    20101 acatcactgc cactgacgtc atcagcggcc tctcaggctc taagacattc gagctgacga
    20161 ggtaatccac gataaccaga accctttcgg ctcattcggc ttcatcagcc acctgcccta
    20221 cttgcccact cggcagtggg gaccccgtga gggactgcct gtcctctgag cctcgatacc
    20281 cagacagcac taaaagagtt gtttgggttt tccgtacctc cacggaggtt gcgccggccg
    20341 actgggtaga gacgtccggc cgcgccactt gccgctcact caggcccgcc ggcgcagcac
    20401 ggcgatgatg ccggcggcaa gcagactgat catgccgggt tccggtacgg cgatggcgaa
    20461 gttatcgatg ccgacaatgg tactgccttc gctggccaga cgcacctcgg tcgtgagaac
    20521 gccggccagt acggtcgcaa acggtggact cggatcagca gcgggattga tatcattgtc
    20581 ggtaagccat ccggcggcct gcgcttgcag gtctgtccag gtcggatcga agttgaccac
    20641 gtaggtgttc cagacgttgg taggaaagct gttggtcaac ggatacagcc agccgttttc
    20701 cacgatgctc ggcctgaagc gaatccagcc tgccgtgata ttgtccgtct tgaagttcag
    20761 atcaaccgag atctgcccaa tacctgcggc ggcatagtct cccgtaaagt ccggatcaag
    20821 gggcatcgcc ccgatgtcga agaccggcgg cgtgagaacc ttgcgagtct cgatgtagcc
    20881 gtccggattt cctcctccgc cgacatgcac cacgacggac gaggtggtgt tgggcacaaa
    20941 accggccgtg tcggtgtcaa acgtctccac gcccggaaca gcaccaagca cgatccaagg
    21001 ccagacaaat actgtcagac cactggcgag aaacagacgt tctgtgcgct tcatgacttg
    21061 ctctcccaaa aatgacccgc aacctgcctt ttccggccgg accaaacaga agaccggcca
    21121 cacaatgcgg catcttagaa ccctacggcc agacatctgt gcggcttgcg cgcatgtttc
    21181 tggatcacag aaagcttgag ttgcggcccg caaaaaggcg gtccgcctta ggacccacca
    21241 aaggctgaat ccgcaaagac cgtcgatctc gcaagaaaac gccatacccg gtgggagtcg
    21301 ccgcacaaac atccgcgaga acagatggac cgcgactttg ccatatgaac ggcaacaaca
    21361 agagcttacc gaaagaaaag gcatgggacc ggctacgaag acgcgctccg caaatgatcg
    21421 tgttgtcaca gtcatcggac aaccgtgata cccatcaccc gacgccggaa gaccttgttt
    21481 cgtaccgggc ggcttatcga atagcctcgt ccgtctggca caacctgccg atttcgccat
    21541 ggggccccca acaccccgag ccccacacga aagagcaatg ccgggagtcg atcctcggag
    21601 ggacgtcctc ggggccgatc aggaattcct gtgccgaacc gttgcacctg accaaccgga
    21661 tcaagcgcat ccgcgccgca agaggcccaa tgctcccctg aggccggtgt gagcgtcacc
    21721 gcagccgccc agccgggcaa caaagccctc cgcgccgagc agatggcgaa gttggagtat
    21781 ggatccgaga attgcgggtt ggaggctgcg aattttcatt caccactctc cattcaaggc
    21841 gggtacaatg cagcgcatgt cgatcgatca ccgcacggtc tcgtgcccat cagcgggttg
    21901 tgttgccctg tggatcgtgg ctttggcatg gggtgcacag gcgatcgtga cccagtcgct
    21961 gctgttgcgt gaggcaatgg tgctcatgtc cggcggcgag cttgtctggg ggatcctgct
    22021 gtgcgcgtgg ctggccggtg tggcggccgg agcgtggatc ggcggccggc tggcccgccg
    22081 gacgtcccgg gccgagcgtc gcttggccat cgcgttgctg ctactgggca tcctggcttg
    22141 tgcgagcatc tggagctttc gcgaggcccg cgttgcgtgc ggcatcggac cgggtgaact
    22201 gctttcgctt gggacgatgg tgatcgtcgc aatcttgctg gtgctgccct gcggcctcat
    22261 ggtcggtatg gctttcccgc ttgccagtgc gatcgctgac agggcccaga cgcccggcgc
    22321 tggaatctcc gtcaaccgca tttacgctct cgaatcggcc ggcagtctca tcggaggggc
    22381 gatgttcacc ttctgggccg tcgagcacct gactccgatt caaaccgtgc tgttctgtac
    22441 cgcgatcacc agtgcgacgc tggctgtgct gctggttcgc gccggatcgc gacggatagc
    22501 tgccgcggtt gtggcgggtc ttgccggccc agtggtgttg acggcgtttc tggccggccc
    22561 tgtaatcaat caatttctcg tggaacgtcg atggcggggc ctgtcgccga catgcgaact
    22621 ggtcgccgaa gcggagtctc ggtatcagaa cctggccgtc ggccggatgg ctgagcagta
    22681 caccctgtat tgcgacggcc agatcgccgc cagtttcccc gaccctttca ctttcgtgcc
    22741 gctggcgcac ttctggatgt gccaacaccc cgacccacgg cgagtactgc tcttgggcgg
    22801 ggcagcagag ggactgctga ccgaggtact caaacatcca gttgagcatg tcgattgcgt
    22861 cgagcccgac ccgagacaaa ttgacctgat cctgccgcta ctaccggaac aggaccggac
    22921 cgcactgcat gatccacgag tgaccgtgca ccacgtcgac ggccgtttgt tcgtcaaaaa
    22981 gcggcaagcc cgcttcgacc tggtcattgc ccgcctgccc gagccgactg ccgcgttgcg
    23041 ggctcggttc tacacccgag agttcttcaa tgagctcagg cggaacatga acgaccagtc
    23101 agtcttgtgt ctgacggcca ccgccggtca ggcgcggctt tcgtccgaat cggcccgtta
    23161 cctggccggt ttgcg
//
LOCUS       DAOTPW010000068        22815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2325_length_22815_cov_5.085062, whole genome shotgun sequence.
ACCESSION   DAOTPW010000068 DAOTPW010000000
VERSION     DAOTPW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22815
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_22815_cov_5.085062"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..166
                     /locus_tag="P5316_08090"
     CDS             17..166
                     /locus_tag="P5316_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84954.1"
                     /translation="MMELAGVKDVLTKVYGSTSPKNLVKAAFEGLAALRTKEDVQKLR
                     EVAVR"
     gene            192..641
                     /gene="rplO"
                     /locus_tag="P5316_08095"
     CDS             192..641
                     /gene="rplO"
                     /locus_tag="P5316_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRR84955.1"
                     /translation="MMIAEITSKAKRNRRRKRVGRGPGSGHGKTSTRGHKGEKARTGT
                     RGHVLREGGQMLFFRRIPKRGFSNFRFRRTFQVVNIAALQETFEDGAKINPSVLAEAG
                     LIRSAADPVKILGVGDLSKKFDVEATCFSTSAAMKITRAGGQIKSLG"
     gene            673..2022
                     /gene="secY"
                     /locus_tag="P5316_08100"
     CDS             673..2022
                     /gene="secY"
                     /locus_tag="P5316_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRR84956.1"
                     /translation="MFSTLANIFRIPELRKKLMFTLGLLIIYRLGFYIPLPGINQDEM
                     AKAAEAAGGGTGFGQMAAVFALFTGGDLQQSTIFGLGIMPYISASIIFQLLATVVPWL
                     EKLQKEGESGRKKINEYTRYATVGLCLIQGVFWVNYMINSRYVYRDFEDSVLFMMMAV
                     MALTTGTIFLMWLGEQIDEYGIGNGISLIIMAGIIARMPNAVIEVYQRADFSVGAGAP
                     QGAMTPLKIIFILVAFVAVVAGAILITQAQRRIPIQQAKHTRGRRVLGGQRQYLPLRV
                     NHGGVMPIIFASSLMIFPGILMSYLASAFGGSNILRILSDQLRTGSYLHELGYIGMIY
                     FFAYFWTAVQFQPKEMANNLRDYGSFIPGLRPGKRTADYLERVMTRITYVGATFLAII
                     AVIPTVVFVSLDVPYQISAFLGGTGLLIVVSVALDVVQRIEANLVMRNYKGFLGASD"
     gene            2053..2712
                     /locus_tag="P5316_08105"
     CDS             2053..2712
                     /locus_tag="P5316_08105"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRR84957.1"
                     /translation="MNLVILGPPGAGKGTQAVRLAQAFSLRHLSSGDLLRAERARGSE
                     LGKKAAEYMDAGKLVPDEIIIGAVLAQLSSLGDKAGLLLDGFPRTVAQAESLDKALAE
                     AGKRVDAVPALAVPDEMILDRITGRRTCPRCNAVYHVKTLPPAQEGICDADGTALVQR
                     PDDTAEVVRQRLDAYHRQTAPLEAYYRKKGLLVEVDGSLEMDAVFRALSKAVRRRLGM
                     E"
     gene            2717..3523
                     /gene="map"
                     /locus_tag="P5316_08110"
     CDS             2717..3523
                     /gene="map"
                     /locus_tag="P5316_08110"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRR84958.1"
                     /translation="MRTIKRRSRTAGLKTPEEIERMRRAGRLVHQVLRRIEQYVRPGV
                     TTAELNAVAEDMIRQAGAEALFRGQRNDRARFPFPAALCTSVNEQVVHGIPGERVLAE
                     GDVVSVDCGVRLEGYCGDAATTIPVGTVSEPVKRLLRVTREALGIAVARIRPKVWWSE
                     IAAEMQAHVEAAGFSVVRDFVGHGIGSSMHEEPKIPNYVDPRERRLDFLLTEGMTLAI
                     EPMVNMGSPQVVYEDSTGWPVVTKDRQYAAHFEHTIAVTGHGADVLTDGR"
     gene            3552..3665
                     /gene="rpmJ"
                     /locus_tag="P5316_08115"
     CDS             3552..3665
                     /gene="rpmJ"
                     /locus_tag="P5316_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRR84959.1"
                     /translation="MKVRSSVRRICENCKIIRRRGVIRVVCSNPRHKQRQG"
     gene            3699..4088
                     /gene="rpsM"
                     /locus_tag="P5316_08120"
     CDS             3699..4088
                     /gene="rpsM"
                     /locus_tag="P5316_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRR84960.1"
                     /translation="MPRIAGVDVPNEKRVEIALRYIYGIGPHLARVVLKEAGVDPNTR
                     AKNLTEDEVSRIAGVIDRNYLVEGQLRRQVQQNIARLRDIGCYRGLRHRRGLPVRGQR
                     TRSNARTRKGPKKTVAGKKGVKEMHRG"
     gene            4081..4476
                     /gene="rpsK"
                     /locus_tag="P5316_08125"
     CDS             4081..4476
                     /gene="rpsK"
                     /locus_tag="P5316_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRR84961.1"
                     /translation="MGKAAAGTVVRRKARRSVARAIAHIKATFNNTQITITDTNGEVL
                     TWASAGTVGFKGTRKSTPFAAQRAAEKVAHAARKFGVMEVEIRVKGPGPGRESAITSL
                     QAAGLRVLSIEDVTPLPHNGCRPRKRRRV"
     gene            4635..5267
                     /gene="rpsD"
                     /locus_tag="P5316_08130"
     CDS             4635..5267
                     /gene="rpsD"
                     /locus_tag="P5316_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRR84962.1"
                     /translation="MSRYIGPVCRLCRREGIKLMLKGPRCETAKCPMEKQWRSNPPGM
                     HAWRRGKSSGYSVRLREKQKVKRYYGILERGFRLYFRRAERSKENTGTALLRLLECRL
                     DNVVRKAGLAPSRKAARAMISHGHIYVNGRKVDRPGFSVSQGDRITVKNSDKSRKLAK
                     SHLELAGSLQPQSWLQVDADKMEATVVALPSRDDVQIPVEEQLIVEMCSR"
     gene            5416..6540
                     /locus_tag="P5316_08135"
     CDS             5416..6540
                     /locus_tag="P5316_08135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRR84963.1"
                     /translation="MRIRWRGLELPTQVVRDDTISTDTYARLVVEPFERGFGTTVGNS
                     LRRILLSSLEGAAVTKVRIAGASHEFMSLPGVMEDVTDIILNIKSLVIRMEGEGEGEK
                     TMHLSRRGPGEVYARDIEADPSLTIINTGQLIATLTADVELNIDFWVSSGRGYRTADE
                     NRNPADELGVIAVDSVFSPVTRVRYKTEETRVGQRTNYDKLIMEIWTKGTIRPEDALV
                     EAARILRKHLNPFVQYHELGREQVAAPSEAEATGGMMDKALQDRLSMPISQLDLSVRA
                     SNCLESAKVTTVGELARMTETDLLKVRSFGRTSLREVKRKLADLGLSLGMVFGDAPAQ
                     QTPAGGSDTGEAAPTTMATDAPAAPAVDAGTVGSTAQMGQ"
     gene            6647..7291
                     /gene="rplQ"
                     /locus_tag="P5316_08140"
     CDS             6647..7291
                     /gene="rplQ"
                     /locus_tag="P5316_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRR84964.1"
                     /translation="MRHLVARRKLNRTSEHREALRRNMAQSLFEHGQITTTLPKAKDL
                     RPFVERLITLGRKARSGNLAARQRLAALLGDRSMIPSGYQEQYEDMTYAARAKVLRMR
                     SGRRYRLGQARGGQKFTAVSIVHHLINEVVPRFEGRPGGYTRVIKLGPSGRNDNAQMA
                     VLQLLGEEESPGTVTRPEKTARQRRTERRYAAAAKAAKKSRSTQSQPTAEAQGE"
     gene            7377..7799
                     /locus_tag="P5316_08145"
     CDS             7377..7799
                     /locus_tag="P5316_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRR84965.1"
                     /translation="MIDASCVFCRIIAGQIPCMRVFEDDAALVFLDIGPLSEGHLLMV
                     PKQHVERLDQMSPEHVMAITRHLPMLGRSLMKAVGAAAYNILQNNGRESGQEVPHVHF
                     HVIPRKAGDGLGYRWNASKYAPGRAEEIRDLLIAALKP"
     gene            7917..9605
                     /locus_tag="P5316_08150"
     CDS             7917..9605
                     /locus_tag="P5316_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR84966.1"
                     /translation="MEWLFSNEGFRAVFLATYVIILLIVSVYGLHRYQLVHLFYKYRS
                     NKPKLTACFRELPRVTIQLPMYNEQAVARRVIDATCRIDYPRDRLEIQVLDDSTDETV
                     QIVGEAVKYWRSQGFNICHLHRTQRTGYKAGALSEGMKTATGEFILIFDADFLAPREI
                     LRETIHYFCDPRVGMVQARWEHLNRDQSLLTKTQAILLDGHFVIEHAARNRSGRFMSF
                     NGTAGLWRKTCIEDAGGWQHDTLTEDLDLSYRAQMKGWKFLFLSELASPAELPPDMEA
                     FKQQQYRWAKGGAQTARKLLPTVLRSRLPRRIKMEAFFHLTSCIVYPCIVLLTLMMYP
                     IVYLKVSMFQEGFWKYLFDFSILILATCSAGTFYVCSQRVLFRTWADSLKYIPFLMAL
                     GIGIALNNARAVFAGFFGKPGEFVRTPKFGVVARDHRWKRQVENARKSLKRVKLQPFV
                     EFAIAIYLLGCVIMCFSAPLNPADPKQQRLTIGVPFLCLFMVGYFYVPLTTWFGHRLG
                     KIESELPEDLAAVPVKFSLPNTQVARGGGSAASPDYAPLVKPDVPAISRQERKV"
     gene            complement(9793..12075)
                     /locus_tag="P5316_08155"
     CDS             complement(9793..12075)
                     /locus_tag="P5316_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR84967.1"
                     /translation="MSTLGTAAGDLRANFLAMAQEADLYPFISTVEMYLAQVPDDWPM
                     RGLAMKALAGKGLLSIAVEIGRACPRSGPEAGQLLDAAEQLAKVPNEIVPWAKTHNRF
                     AANLAALRSRGQNQAMLADALANVWPAMEKDLTLHQANDGNLLVRGSRSDGTRMWIPA
                     ALDFRNAVEHIAKTDAFKGKCGPPLLIDGVGMGWWLSGLYAMTARTFLDYSSTLYIVE
                     TNLKALALVLRLHDWSAELADPRVYILGGPDAWETWRNMMIADTTLPLPSTCQATVRW
                     PGQKPTPGQERLAEVNRHRIDHLIELRRRAEEIYADRDAAWWARRYAAAGPQDPLRVL
                     CVTSRFTTFLKYSMRDTVSALEGAGIRTRLLIEAQDHLILGPHAYMETFIEFQPDLVL
                     MIDHHRHEQPNAFINNVPFACWIQDPLPNLMCRRAGEALGPLDFTFGYYRNRCTREFG
                     YPADRFFTAPIPVADAMFSEEPVSDEEAKRLACDVMYVGHLNQTIEALRTHWRAEQPR
                     ELHPILDDIDAFVDRVLERGEHLREHYSNTDPTELVRRLAGDRGKHLRPEDAERLFTF
                     YANRTFDIGFRVRTLQWVARWARDTGRVFRLYGNGWQHVRELAPFAVGPIEHGEPLRR
                     AYRCATFAIQTMPAGFRHQRSLEAIAGGCLVLTRYIATDFGGLDLEEARRRRKAGEPL
                     PATAASFPRLDMVTFRDPQEMASVAQELLDKPDLRRELLAEFRERVLQEFSYGRVIRT
                     VIEYIRDALQQQASVRQGST"
     gene            12376..12594
                     /locus_tag="P5316_08160"
     CDS             12376..12594
                     /locus_tag="P5316_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84968.1"
                     /translation="MICQERPPKIVLAIWMAVIIIVMAAAWIVIGARSGFMAGLLWAA
                     VIWVMLGGVEVTYFILHHRSRDTAPPPT"
     gene            complement(12931..13128)
                     /locus_tag="P5316_08165"
     CDS             complement(12931..13128)
                     /locus_tag="P5316_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRR84969.1"
                     /translation="MAEVNKVSIDASECISCEACVDACPDVFEMGKDAAVVKAGADIQ
                     ANSDGIVSAAEACPTSAIKYE"
     gene            complement(13502..15454)
                     /locus_tag="P5316_08170"
     CDS             complement(13502..15454)
                     /locus_tag="P5316_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRR84970.1"
                     /translation="MAGISDRIRSSQIWKSVVRHGLPRDRQGRALVVVSNLFLHIHPV
                     DMRRYSLRFWFTWAMGWLTFVLFVIETITGIALMFYYRPAAEHAYADMQALRATVSFG
                     LVREIHRWGAHAIVIAAWLHMLRVFLTGSYKPPREFNWCIGVVLLVLTMLLSFTGYLL
                     PWDQLALWAVTVGTNMVSAAPVIGSDGPWSGRLPAGWDPKSLLLGGRDVGPPALLRFY
                     VLHCAVLPVISAIFISLHFWRIRKDGGISGPDELYTPSQARRRSNRTALVLALAALTA
                     LLLTAVFGSWSGIKGLVNWYCRPERLLPIAIAVLVIGLAAYRWWSRPVFAAILGFLFL
                     AGWATACADPQFSWIILQPDNVAISLMVCATIGFLWLAARRMAINDQRLGRGLPPCEA
                     EDRLRVMVWPDVLRVELVIFLIAAAGLIVWSILLRAPLEAAANPADVPNPSKAPWYFV
                     GLQELLVYFDPWLAGFLIPTLTIVGLCALPYLDVNRRGNGYYTLRERPFAVSVFLFGF
                     LILWLLPIVIGTFLRGPNWSFFGPFEPWDPQQTAVATCVNFSDLFWTHALGRSLPSPE
                     STGTAASLLRELPGLIALAAYFLGVPLILKRTWMRKLALQMGKPRFLALMLLLTTMGL
                     LPIKMLLRWMFHIKYIVSFPASAFNI"
     gene            complement(15619..16122)
                     /locus_tag="P5316_08175"
     CDS             complement(15619..16122)
                     /locus_tag="P5316_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRR84971.1"
                     /translation="MSNDPQVPRRRILSAGGIGWLALGASLGAGTWATARFLRPSPAS
                     RQENEVDAGPLDRYAALPSGQISDAVSDKAIWIVRMAKRIVAIRAACTHLGCRVAWVS
                     NDGRFRCPCHGSSFDLDGTNLDGPAPRPLERLAIRVQDDHVIIDSNRRFRKERGEWDH
                     PDSFISA"
     gene            16978..17574
                     /locus_tag="P5316_08180"
     CDS             16978..17574
                     /locus_tag="P5316_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84972.1"
                     /translation="MKKAMVFVLLAMAAPALAGNLNLADAKAWGAPWGQAATVSDQGN
                     GVFNLSIGYGSAGVFWRLPAFPSEIVRITGTWTGDCGGAGWAEVMMFTSTEGMSDAEI
                     ANRIDTGNAADIVAKHDSWGLNGGPAWPWQAIEAAPMTPPGGKYEIHATCAEVVVALK
                     VGQVGQPGTWATYDLTFIPEPAAALLLGLPLMLLRRRR"
     gene            complement(17606..19861)
                     /locus_tag="P5316_08185"
     CDS             complement(17606..19861)
                     /locus_tag="P5316_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR84973.1"
                     /translation="MRSAPKPPGDEGGTGSDRPDTTEAVTTSRVLPAVMLVAVTIIVF
                     FAHRRALTAQALSMNDGDYVVQNYRVQNPSWRHAGLFLAEVTRPAMVEGYYQPLAMIS
                     LMLDSAMGGRPDHLMPYHRTSLVLHVANTVLVIILLYLLFHRPVPAVLAGLLFGLHPM
                     TVEVVTWLGERKTPLATFFALISMVLYVRYARSGSRGIYAASLVMFLLALLSKPTVTP
                     LPILLLLLDYWPLGRLDWRRLIEKLPFFAIAVASAIITVISQRHVDLTAMQGISFEQN
                     LLIVCHNVLFYPLKMIWPANLCSVYPFPEPIDLSNRSVFLGAVSTVLLAGGLVISLRW
                     TRALMAGWLFFLVALAPTLLNVGYAMGVAADKYAYLPAVGFLLILTWLLSRLWNIAQA
                     GVARRLCRSAVVVVPLGAAACYAVAADRYQLKWQDPGTYCEHILSIAPKTAWAHQALA
                     SHLIDQNRIEDAVRHARQAVELDPDNYKVRITYGEALSRQNDTTEAMSQFTRALAIKP
                     ESAEAHNGLGMACGMAGQWDEASRHFEAALQSQPHSPEILFNLATAHLYAGQFDKALA
                     RLAEVSRYRPDSPEVTDRLAQTHYSWGQRLARENRLNDALPHFEKAAALRPNELQAYY
                     ALAYAYMGLDQSDQAISAFRKVVALAPKHADAHCRLGDLYLRKGQSELAAKAYQTALQ
                     VDPHNQKAKAGLEAAQSTQPASSLRSGRPHAIPPTQAGDGPQVHSAKNACDAHKRGRR
                     IFQIRRPPFLA"
     gene            complement(19962..>22815)
                     /locus_tag="P5316_08190"
     CDS             complement(19962..>22815)
                     /locus_tag="P5316_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84974.1"
                     /translation="DDDYGSNSGSAYVLRREGLKWVHQAKLYASDAGADDKFGRCVAI
                     SGDYIIVGAPDKDDAGADSGAAYIFTRTDASWTEQAKLVASDAAAGDKFGSSVAIAGD
                     FALVGAYGDDDNGSASGSAYVFLRNGDTWTQQAKLKPDDGAAGDSFGWAVSICGNHAV
                     IGAYGDDDTVTGSGSAYVFFHDGDNWTQQAKLVAADPQAFAGFGLSVSISGDHAIVGA
                     PWTDDGGNDSGSAYVFERQGTQWAQAEKLSAANAETGAMFGHSVSISGDSIVVGTLYD
                     DEGGNNAGAACVFHRRASAWIRKTKIIASDAAAGDQFGQAVCVNGDFAIVGSPYDSDS
                     GTHSGSAYVYEIGPASASLTITVCQGGGGDFSTIQAAIDAAVPGDVISICDGVYTGPG
                     NRDLDFGGKAITVRSQNGPAQTIIDCEGAGRAFRFHSGEGASSIVDGITMINGSVLAA
                     GQDGGAILCVGSSPTIKGCVIRNCTAGFGGGIACDAASPTIVDCRFYGNIADYGSGGG
                     ISCCNASHAIIDGCDFRGNRAVSGGGIRCYQSNPIITNCNISGNHGNTGGGIYCNRSE
                     AVISGCSLVGNHSYFGGGMYCYYNSPVIANCIVSGNTCVTSGGGLYCSTNAPAVTNCL
                     VSGNFAPANGGGIQGYLANPVFTNCTVSANMAGGDGGALACNQSSATTVNCILWDNTA
                     ARGPEIAVFAGGSLTVSYSDIHNGQAGAYVESGSTLSWQNGNIDADPLFMGGTSGIWT
                     APGTYDPDTFEITFTDANATWTPNQWVGKLVNPDTTQPLQMLIVANTANTVTVLAERN
                     TIELGASWITGAESYQIYGFHPQAGSPCINGGDTLALPPDKADLDHDGDASEPVPVDL
                     EARPRIQGCVVDMGAYEFEVPARLPGDLDNDCDVDQDDVAALLACLTGNGIPQYNPAC
                     SDARLDEDLDVDQADFGLLQRCLSGPDTLPDPHCAD"
BASE COUNT         4359 a         6538 c         7509 g         4409 t
ORIGIN      
        1 agccagcgtg cgggcggtga tggaactggc gggtgttaag gacgtgttga ccaaggtcta
       61 cggttcgacc agtccgaaga acctggtcaa ggcggcgttc gaggggcttg cggccctgcg
      121 gaccaaggaa gacgtccaaa agcttcggga ggttgcggtc agatagggcc gccgggcggg
      181 agtgacaggc catgatgatt gctgagatta cctcgaaggc caagcgcaac cgacgtcgca
      241 agcgggtcgg tcgcggtccg ggttcgggtc acggcaagac cagtacccgc gggcacaagg
      301 gcgagaaggc ccggaccggg acgcgtgggc atgtgctccg cgagggcggc cagatgctgt
      361 tctttcgcag gattcccaag cggggctttt cgaacttccg tttccggcgg acgttccagg
      421 tggttaatat cgcagcgttg caggaaacgt tcgaggacgg agccaagatc aacccttcgg
      481 ttctggcgga ggccggtctg atccgaagtg ccgccgatcc cgtgaagatc ctcggcgtcg
      541 gtgacctgtc gaagaagttt gacgtcgagg caacctgctt cagtacgtcg gccgcgatga
      601 agatcacgcg agcggggggc cagatcaagt cgctcggctg aggccggccg gaacgactgg
      661 aggcatgcgg gcatgttcag cacattggcg aacattttca ggatccctga gctgcgcaag
      721 aagttgatgt tcacgctcgg gctgctgatc atctatcgcc tggggtttta cattcctttg
      781 cccggtatca accaggacga gatggcgaag gcggccgagg cggccggggg cggcacgggc
      841 ttcgggcaga tggccgcggt tttcgctctg tttaccggcg gcgatcttca gcagagcacc
      901 attttcgggc tcgggatcat gccgtatatt tccgcctcga tcattttcca gctcctggcg
      961 accgtggtgc cctggctcga gaagcttcag aaggagggcg aatcgggccg caagaagatc
     1021 aacgagtata ctcgttacgc caccgtcgga ctgtgtctga tccagggcgt gttctgggtc
     1081 aactacatga tcaactcgcg ctacgtctac agggactttg aggattcagt tcttttcatg
     1141 atgatggccg tcatggctct gacgacgggg accatattcc tgatgtggct cggcgagcag
     1201 atcgacgaat acggtatcgg caacggcatc agtctgatca tcatggccgg tatcattgcc
     1261 cgaatgccca atgccgtcat cgaggtctat cagcgggcgg atttcagcgt gggcgcgggg
     1321 gccccgcagg gtgcgatgac gccgctcaag atcatcttca tcctggtagc gtttgtggcc
     1381 gtggtggccg gcgcgattct gatcacccag gctcagaggc gtattccgat ccagcaagcc
     1441 aagcacaccc gcggccggcg tgttcttggc ggccagcgac agtacctgcc cttgcgggtc
     1501 aatcacggcg gcgtgatgcc gatcattttc gccagttctc tgatgatttt tccgggcatt
     1561 ctaatgagtt acctggcgag cgcttttggc ggcagcaaca tcctgagaat cctctccgat
     1621 caattgagga cgggcagcta tctccacgag ctgggctaca tcggaatgat atacttcttt
     1681 gcctacttct ggaccgcggt tcagttccag cccaaagaga tggccaacaa cctgcgggat
     1741 tacgggagct tcattcccgg cctgcggccc ggcaagcgga cggcggacta ccttgaacgg
     1801 gtgatgacgc gtatcaccta cgtgggtgcg accttcctgg cgatcattgc ggtgattccg
     1861 accgtggtgt tcgtgtcgtt ggacgttccc tatcagatct cggccttcct ggggggcacg
     1921 ggtttgctga tcgtggtaag cgtggccctg gacgtggtcc agcggatcga ggccaatctg
     1981 gtgatgcgca actacaaggg gttcctgggc gcctcggatt agtgggaggt tcccgaaggg
     2041 catgcgatgg gtatgaatct ggtcattctt ggtcctcctg gagccggtaa gggcacgcag
     2101 gccgtgcgac tggcccaggc cttttcgctg cggcacctgt cgagcggcga cttgctgcgg
     2161 gccgagcggg ccagagggtc cgagttgggc aagaaggccg cagagtatat ggacgcgggc
     2221 aagctggttc cggatgaaat catcatcggg gcggttctgg cccagttgag cagcctgggt
     2281 gacaaggcgg gtttgctcct ggacgggttc ccccgaacgg tggcgcaggc cgagagcctg
     2341 gataaggcct tggccgaagc cggcaagcgg gtggatgcgg ttcccgcgct ggcggtgcct
     2401 gatgagatga tccttgaccg gatcaccggg cggcgtactt gtccccggtg taacgcggtt
     2461 tatcatgtaa aaaccctgcc gccggcacag gagggcattt gtgacgccga tggcaccgca
     2521 ctggttcagc gtccggacga cacggctgag gttgtgagac aacggttgga tgcctatcac
     2581 cgacagactg ctcctttgga agcttactat cggaagaagg ggcttcttgt ggaggtggac
     2641 ggcagcctag agatggacgc agtattccgg gcgctgtcga aagcggttcg ccgacggttg
     2701 ggaatggaat gagccgttga gaacgatcaa gcgacgatcc cgaaccgcag ggttgaagac
     2761 accggaggaa atcgagcgga tgcgacgggc cgggcggctc gtccatcagg tgcttcgccg
     2821 gatcgagcaa tacgtgcggc cgggcgtgac gacggccgaa ctgaacgcgg tcgccgagga
     2881 catgatccgg caggcggggg ctgaggccct ctttcgaggg cagcgtaacg accgagcgag
     2941 attcccgttt cccgcggccc tgtgcaccag cgtgaacgag caggtggtac acgggatacc
     3001 gggtgagcgg gtgctcgcgg aaggcgacgt ggtcagtgtg gactgcggcg ttcggctgga
     3061 gggctactgc ggggacgccg ccacgacgat tcccgtgggc acggtgagcg aaccggtgaa
     3121 acggttgctg agggtgaccc gggaggcgtt ggggatcgcg gtggcccgta ttcgcccgaa
     3181 ggtgtggtgg agcgagatcg ccgccgagat gcaggcccat gtggaagcgg cgggtttttc
     3241 ggtggtacgt gattttgtcg gccacggaat cggcagcagc atgcacgagg agccgaaaat
     3301 acccaactat gtcgatccga gggagcggcg actggatttc ctgctcacgg aaggaatgac
     3361 gctggcgatc gagcccatgg tgaacatggg atcgccgcag gtggtttatg aggactctac
     3421 ggggtggccg gtggtgacca aggaccggca gtatgcggcc cattttgagc acaccatcgc
     3481 ggtcaccggt cacggggcgg acgtgttgac ggacgggcgg taggcgtgca agcgcggcgg
     3541 aggaccgaaa tatgaaagtg cggagcagtg ttcgtcgcat ttgcgagaat tgcaagatta
     3601 tccgtcgccg gggcgtgatc cgggtggtgt gctcgaatcc gcgtcataag cagcgtcaag
     3661 gctgacggta ggagcctgtt gcaggaagga gtggcaacgt gccgcgtatt gcaggcgttg
     3721 atgtgccgaa tgaaaagcga gtcgagatcg cgctgcggta tatctacggc attggaccgc
     3781 atctggcgcg ggtggtgctg aaagaggcgg gagtagatcc gaacacgcgg gcgaagaatc
     3841 tgacggaaga cgaggtcagc cgaatcgccg gcgtgatcga ccggaactat ctggtggaag
     3901 gtcagcttcg caggcaggtg cagcagaaca tcgcccgtct ccgtgacatc ggttgctacc
     3961 ggggtttgcg tcaccgtcgc ggcctgccgg tccgcggcca gaggacgcgc agcaatgccc
     4021 ggacccgcaa gggtccgaag aagaccgtgg ccgggaagaa gggcgtgaag gagatgcatc
     4081 gtgggtaagg cagcagcggg aacggtcgtc aggagaaagg cgcggcgcag cgtggcccgc
     4141 gcgatcgccc atatcaaggc gaccttcaac aatacgcaga ttaccatcac tgacaccaac
     4201 ggtgaggtgc tgacatgggc gtcggcgggg accgtgggct tcaaaggcac gcggaagagc
     4261 actccgtttg ccgcgcagcg agccgccgag aaggtggcgc acgcggcccg caagttcggc
     4321 gtgatggagg tcgagattcg cgtcaaaggt cccggcccgg gacgggagtc ggcgatcacc
     4381 tcgttgcagg cggcggggct tcgggtgctg agcatcgagg acgtgacgcc gttgccgcat
     4441 aatggttgcc gtccgagaaa gcggcgccgg gtgtgattcg ggcggtttgg gagctgccgg
     4501 acacgggagt tcggctgaac gagcggggac ttgagacggc aggcagggtt gaacaagaac
     4561 ggacgccgtg gtggatgacc cgttcaaggc aggttgatcc ggttaacgga cgcagcacaa
     4621 gggaaacaga atagatgtcg cgatacatcg gtccagtctg tcggctttgc cggcgagaag
     4681 gcatcaaact gatgctgaag gggccgcgtt gcgaaaccgc gaagtgcccg atggagaagc
     4741 agtggcgatc gaatccgccg ggcatgcacg cgtggcggcg cgggaagtcc agcggctaca
     4801 gcgtgcggct tcgtgagaag cagaaggtga agagatacta tggcattctg gagcgcggtt
     4861 tccggctgta tttccgtcgg gcggaacgat ccaaggagaa caccgggacg gccctgctcc
     4921 ggcttctgga gtgccgcctg gacaatgtgg tccgcaaggc gggtctggct ccctcgcgca
     4981 aggcggcccg ggcgatgatc agccacgggc acatctacgt caacgggcgg aaggttgatc
     5041 ggcccggctt cagcgtcagc cagggcgacc gcatcaccgt gaagaactcg gacaagagcc
     5101 gcaagctcgc caagagccac ttggaactgg ccggctcgct gcaaccgcag tcctggttgc
     5161 aggtcgacgc cgacaaaatg gaggcaaccg tggtggccct gccgtcacgg gacgacgtcc
     5221 agattccggt tgaggaacaa ctgatcgtgg aaatgtgcag ccggtaacgg cttttcgtgg
     5281 gtgcgggccg agtcgaaagg ggtacccgac gtcgttcatc agaggccggt tcgtccggcc
     5341 gcagatgaaa gcccggtgat cctcgaatcg agcgccgttt gcatcgagct tgcaggcaat
     5401 tgtggaggaa tcccaatgcg aatcagatgg cgtgggcttg agttgcctac tcaggttgtt
     5461 cgggatgaca cgatcagtac ggacacctat gcccggctcg tggtcgagcc gtttgagcgg
     5521 gggttcggta cgacggtcgg gaacagcctt cgtcgaatcc tgttatcgag cctcgaaggc
     5581 gctgccgtta ccaaggtgcg gatcgccggg gccagccatg agttcatgag cctgcccggc
     5641 gtgatggaag acgtgaccga cattatcctg aatatcaaga gcctggtcat tcgcatggag
     5701 ggcgagggcg agggcgagaa gacgatgcac ctgtcccggc gcgggccggg ggaggtttac
     5761 gctcgagaca tcgaggccga tccgtctctt acgattatca acaccggcca gctcatcgcg
     5821 acgttgaccg ccgacgtcga actgaacatc gacttctggg tcagctcagg tcgaggctac
     5881 cgaacggcgg acgaaaaccg caacccggcg gacgagctcg gggtgatcgc ggtcgattcg
     5941 gtgttctcgc cggtcaccag ggtccgctat aagaccgagg agacgcgcgt cggacagagg
     6001 accaactacg acaagctgat tatggaaatc tggaccaagg gcacgatccg gccggaagat
     6061 gcgttggtcg aggcggccag gattcttcgc aagcacctca accccttcgt ccagtatcac
     6121 gagttgggtc gggagcaggt ggccgccccg tctgaggccg aggcgaccgg cggaatgatg
     6181 gacaaggcgt tgcaggatcg tctatctatg ccgatctccc agttggacct ttcggtccgg
     6241 gcaagcaatt gtctggagtc ggcgaaggtg accaccgtgg gcgaactggc caggatgacc
     6301 gagaccgatc tgctgaaggt gcggagtttt ggccggacct cgttacgtga agtgaagcgc
     6361 aagctggccg acctgggcct gagccttggg atggtctttg gcgacgcgcc tgctcagcag
     6421 actccggcgg gtggttcgga caccggcgag gccgctccga caacgatggc gaccgacgca
     6481 ccggcggcgc cggcagtcga cgccggcacg gtgggcagca cggcccagat gggtcaatga
     6541 ggcgagcagg ttcgaacgac cgggatcgga ggacggctac gggagacttt tgtgagcttg
     6601 ggtcccggcg cgggtgagtc cggcaggtga gttgggagcg tttggaatga gacaccttgt
     6661 tgcacggcgg aaactgaatc gaacgagtga gcaccgagag gcccttcggc gcaatatggc
     6721 ccagagcctc tttgagcatg ggcagatcac gacgaccctt cccaaagcga aggatctgcg
     6781 tccttttgtt gagcggctga tcactctggg gcgaaaggcc aggtctggga atctcgctgc
     6841 ccggcaacgt ctggccgcgc tgctgggtga ccgatcgatg attccctcgg gctaccagga
     6901 gcagtatgag gatatgacgt atgccgcccg cgccaaggtc ctgcggatgc gcagtgggcg
     6961 gcggtatcgg ttgggtcagg cccgcggtgg tcagaagttc acggcggttt ccatcgttca
     7021 tcatctgatc aacgaggtag ttcctcggtt cgaagggcgt cccggcgggt acacgcgggt
     7081 catcaagctt ggtcccagcg gccgtaacga caatgcgcag atggctgttc tccaactgct
     7141 gggagaggaa gaatcccccg gcacggtgac gcggcctgag aagaccgccc ggcagcgccg
     7201 gaccgaacgg cgctacgcgg ccgccgcgaa ggctgccaag aagagccgct caactcagtc
     7261 gcagccgacg gcggaagctc agggcgagtg aggcggaccg tagacttgct gatccggaat
     7321 gcggcaggca actcgaggtc atgccgaacc gcggtaccgc ggcacggagg agtgcgatga
     7381 tcgatgcttc ctgcgtattc tgcagaatca ttgccggtca gatcccgtgc atgcgtgtgt
     7441 ttgaagacga cgctgcgttg gtgttccttg atatcggtcc gctgtcggaa gggcaccttc
     7501 tgatggtccc caagcagcac gtcgagcggc tggaccagat gtccccggag cacgtcatgg
     7561 cgattacccg ccatctgccc atgcttggcc gatcgctgat gaaggccgtg ggggcggctg
     7621 cctataacat tctccagaac aacggtcgcg aatccggtca ggaggttccc catgttcatt
     7681 ttcacgttat cccgcgcaaa gcaggcgacg gtctggggta tcgctggaac gcctcaaaat
     7741 acgccccagg aagggccgaa gagatccgcg acttgctgat tgccgccctc aaaccgtaag
     7801 ccgttgacat tcttattcct aatcgtcatg atcaatttgg ggtggaacga ggcggcgcgt
     7861 ttgggatggc gctcgttctg aaggatgcga ccactcaacc gaggtgtcct ggtttcatgg
     7921 aatggctgtt cagcaacgaa ggttttcgcg cagtctttct ggcgacctat gtcatcatcc
     7981 tgctgatcgt cagcgtttat gggttgcatc ggtaccagct cgtccatctg ttctataagt
     8041 atcgctcgaa caagcccaag ctgaccgcct gtttcagaga gttgccgcgc gtcacgatcc
     8101 aactgccgat gtacaacgag caggccgtgg cccggcgggt gattgacgcg acctgccgaa
     8161 tcgactaccc gcgcgaccgc cttgaaatcc aggtgttgga cgattcgacc gacgagaccg
     8221 tccagatcgt cggcgaggcg gtgaagtact ggcgttcgca gggcttcaac atttgccacc
     8281 ttcatcgcac ccagagaacc ggctacaagg caggcgcgct ctccgagggc atgaaaactg
     8341 caaccggcga gttcatcctg atttttgatg cggattttct cgcgcctcgg gagattctcc
     8401 gtgagaccat tcattatttc tgcgacccgc gggtcggcat ggtgcaggcc cgatgggagc
     8461 atcttaaccg cgatcagtcg ctcctgacca agacgcaggc gattctgctg gacggacatt
     8521 tcgtcatcga gcacgcggcc cgtaaccgct cggggcggtt catgagtttc aacgggaccg
     8581 ccgggctttg gcgaaagacg tgtattgaag acgcgggggg ctggcagcat gacaccctga
     8641 ccgaagacct ggatctctcc tatcgcgcgc aaatgaaggg ctggaagttc ctgtttctct
     8701 ctgagttggc ctctccggcc gagttgccgc cggacatgga agccttcaag cagcagcagt
     8761 accggtgggc caagggcgga gcccagacgg cccgcaagct gttgccgacg gttctgcgct
     8821 cgcggttgcc caggcgaatc aagatggaag ccttcttcca tctcacgagc tgcatcgtct
     8881 atccgtgcat cgtgctgctc actttgatga tgtaccccat cgtgtacctc aaggtgagca
     8941 tgtttcagga agggttctgg aaatacctct tcgatttctc gattctcatt ctggcgacct
     9001 gttcggccgg gacgttctac gtctgcagtc agcgggttct ctttcgtacg tgggccgaca
     9061 gcctgaagta cattccgttc ctgatggccc tggggatcgg tatcgcgctg aacaacgcgc
     9121 gggccgtgtt cgcgggtttc ttcggcaaac ccggcgagtt cgttcggacg ccgaagttcg
     9181 gggttgtggc ccgtgaccac cgctggaaac ggcaggttga gaacgcccgc aaatccctca
     9241 agcgggttaa gcttcagccc ttcgtggagt tcgctattgc gatttacctg ttgggctgcg
     9301 taatcatgtg tttttccgct cctctgaatc ccgccgatcc aaagcagcag cgtctgacca
     9361 tcggcgtgcc cttcctctgc cttttcatgg tgggctattt ttacgtgccc ctgacgacgt
     9421 ggttcgggca ccgcctcggc aagatcgaat ctgaattgcc tgaagacctg gcggccgtcc
     9481 cggtcaagtt ttctctcccg aacacgcagg tggcccgcgg gggcggctcg gcagcgtctc
     9541 ccgactatgc ccccctggtc aagcccgacg ttccagcgat ttccaggcag gagcgaaaag
     9601 tctgagaccg ctcagccgct aaggaaggcc gcggatggca taaccggaca ctcgaaggtc
     9661 attcatgctg ccacggcaag tcttcgacat atggttttcg tcattgactg gcagtcggtc
     9721 gttcatcggg tttgtcaggc aacccgattc gcgaagcggg tttgctcatc aagcagcacc
     9781 gacgggtctt gttcaggtac tcccttgtcg aactgaagcc tgctgctgca aggcgtctcg
     9841 aatgtattcg atcactgtgc gtatgacgcg accgtagctg aactcctgca gcacgcgctc
     9901 ccggaactcc gccaggagtt cgcggcgcag atcgggcttg tccaggagtt cctgggccac
     9961 cgaagccatt tcctgagggt cgcggaacgt aaccatatcg agtcggggga aactggcggc
    10021 tgttgcgggc aggggctcgc ctgcctttcg ccgccgccgg gcttcctcca gatcaaggcc
    10081 gccgaagtcg gttgcgatgt aacgggtgag cacaaggcag ccgccggcga tggcctccag
    10141 cgatcgctga tgtcggaagc ctgccggcat ggtctgaatg gcaaaggtcg cacagcggta
    10201 ggccctgcga agcggttcgc cgtgctcgat gggcccaacg gcaaaaggcg cgagctcgcg
    10261 gacgtgctgc cagccgttac catacaacct gaacacccgg ccggtgtccc gtgcccatcg
    10321 cgcgacccac tgcagcgtcc ggacgcgaaa accgatgtcg aacgtgcggt tcgcgtagaa
    10381 ggtgaaaagc cgctcggcat cctccggccg gaggtgcttc ccgcgatcgc cggccaaccg
    10441 tcgaacaagc tccgtaggat cggtgttcga gtagtgttcc ctcaagtgtt cgccccgttc
    10501 caggacgcgg tcgacgaacg catctatgtc gtccaggatc ggatgcagct cgcggggctg
    10561 ctcggcccgc cagtgcgttc gcaacgcttc gatcgtctgg ttaaggtgcc ccacgtacat
    10621 cacgtcgcag gccagtcgct tcgcctcctc atcgctaacc ggttcctcgc tgaacatcgc
    10681 atccgccacg ggaatgggag cggtgaagaa tcgatcggcc gggtacccga attccctggt
    10741 gcagcggttg cggtaatagc caaaggtgaa gtcgagcgga ccgagcgcct cgccggcgcg
    10801 gcggcacatc aaattgggca gtggatcctg aatccaacag gcgaacggca cgttgttgat
    10861 gaaggcgttc ggctgctcgt ggcgatgatg atcgatcatc agcaccaggt cgggctgaaa
    10921 ctcgatgaac gtctccatgt acgcgtgcgg accgagtatg agatggtcct gagcttcgat
    10981 cagcagtcgc gtccggatgc ccgccccctc gagcgcgctg accgtatcgc gcatcgaata
    11041 cttcaagaat gttgtaaacc gtgacgtgac gcacagaacg cggagcgggt cttgtggccc
    11101 ggcggcagcg tatcgtcggg cccaccaggc ggcgtcgcgg tcggcgtaaa tctcttcggc
    11161 acgccggcgc aattcgatca ggtggtcgat gcgatgccgg ttgacctcgg ccagccgttc
    11221 ttgtcccggc gtgggttttt gtccgggcca ccggaccgtc gcctggcagg ttgacggaag
    11281 tggtagagtc gtgtcggcga tcatcatgtt ccgccaggtt tcccatgcgt ccggtccgcc
    11341 cagaatatac acccgcggat cggcgagttc cgccgaccag tcgtgtagtc gcagcaccaa
    11401 agccaaggct ttcaggttgg tttcgacgat gtagagggtg ctgctgtagt ccaggaaggt
    11461 tctggccgtc atggcataga gcccggacag ccaccaaccc atgccgactc cgtcgatcag
    11521 caggggcggt ccgcatttac ccttgaaagc atcggtcttg gcgatgtgtt ccacggcatt
    11581 gcggaagtcc agggctgccg gaatccacat cctcgtgccg tccgagcgcg atccacggac
    11641 cagcagattg ccgtcgttgg cctggtgcag cgtcagatcc ttctccatcg caggccacac
    11701 gttcgcaagg gcgtcggcga gcatggcttg gttctgcccc ctggatcgaa gtgcggccag
    11761 gtttgcggca aagcgattgt gcgtcttggc ccatgggacg atctcgttag gcaccttggc
    11821 tagttgttcg gcggcgtcga gcagttggcc ggcctcgggg cccgatcgtg ggcaagcgcg
    11881 gccgatctcc accgcgatgc ttaagagccc cttgcccgcc agggccttca tggccagccc
    11941 gcgcatcggc caatcatcgg gcacctgtgc gaggtacatc tccaccgtcg agatgaacgg
    12001 atagaggtcg gcctcctggg ccatcgccaa gaaattcgcg cgcagatcac ccgccgcagt
    12061 gccaagagtg gacattacac cggttatcga ccgattgagg cttgcaataa caccaggcgc
    12121 ccgaaatgac gattcgccgt cacaggcagg cacgcgtttt gtgccggtga accgtttacc
    12181 ggcatggcgg atctggatac atgtcccggc gatgttttct tcggaacaaa acggtgcctc
    12241 gtgtgtctta ttatctgggg aagcattgtc ccgtccgggc tgcggggcgc gatgctatgc
    12301 cgagcacaga tgaggctgcg acgggaggtc ggtgaaagac ccgttgcggc cgcactctga
    12361 catacggggg tggccatgat ttgccaggag cgtcctccga agatcgttct tgcgatctgg
    12421 atggcggtta tcatcatcgt catggctgcc gcctggatcg ttatcggcgc tcgcagcggt
    12481 ttcatggccg gtttgctctg ggccgcagtg atctgggtga tgcttggtgg agtggaagtc
    12541 acctatttca tcttgcacca ccgcagcaga gacacggctc caccgccgac gtgatcagga
    12601 acgctgtccg ggccccgctc ttgtctgagt tcaaggtact ccgatcagga ataccgagac
    12661 ggtgccgaag tatccgagtg cggcagcggc cgaccggcag gggccgtgag tcacatgtgg
    12721 agcttgccgt tcaaggctct gccccttccc gaccagcgta ccattgggca catcgggttt
    12781 tcgcggtgaa atcgccggag gtgggtgcaa tgggcggtga gaccatcgag cgcaatgaga
    12841 accggaagca gcagcgtgtt tttgctgctt ccggtcgtta ttccgagaac gctcctgcta
    12901 aaccgcccgc gaaccggacg gacgcaggca tcactcgtac ttgattgcgc tggttgggca
    12961 agcttcagcg gccgagacga tgccatcgct gttcgcctgg atgtctgccc cggccttcac
    13021 gacggccgca tccttgccca tctcaaacac gtccggacag gcgtcaacgc acgcctcgca
    13081 cgagatgcat tcgctcgcgt caatcgaaac cttattgact tccgccataa tcgtaggttc
    13141 tcctttcgtt gggcggtcct gccgccacat gtccgattgc gttgaccgcg atccgtgccg
    13201 ttcctggcct tgctcggagg cacgtcgccg gctcatttgt tacttgtccc cgaaaaccgg
    13261 ccgcctgtca accgatgaag gattatacgt gtactcctga tcattgcgga accgccggtc
    13321 ggtctggggc ttgattgtca gtcgtttacc gattgctttc ggctcatgcg caccggtaat
    13381 gaggcgaact gtcggcacgc aagccgaggg gggcctggcg gatgttaccg gcgggttgtg
    13441 tgccggcgcc cctcgaagcc gccgaaaagg gccgttgggc ctcctctcca tgtgattctg
    13501 ttcagatatt gaacgccgag gccggaaatg aaacgatgta cttgatgtgg aacatccagc
    13561 gaagaagcat tttgatcggc agcaaaccca tggtggtcag aagaagcatc agggcaagga
    13621 accgaggctt gcccatctgt agggccagct ttctcatcca cgttcgcttg aggatgagcg
    13681 gaacaccgag gaagtacgcg gccagggcga tcagccccgg cagttcccgg aggagcgacg
    13741 cggcggttcc cgtcgactcc ggcgaaggca gcgatcgtcc cagagcatgc gtccagaaca
    13801 gatccgagaa gttgacgcag gtcgcaacgg cggtttgctg agggtcccac ggttcaaacg
    13861 ggccgaaaaa actccagttg gggcctcgca ggaacgtgcc gatgacgatc ggaagcagcc
    13921 acagaatgag gaagccgaac aggaagacgg agacggcgaa aggacgctcg cggagcgtgt
    13981 aataaccgtt gcctcgcctg ttgacgtcca gataaggcaa ggcgcaaagg ccgacgatcg
    14041 tcagggtagg gatcaggaag ccggcgagcc aggggtcgaa atagaccagc agttcctgca
    14101 agcccacgaa gtaccacggg gctttggatg ggttcgggac atcggcgggg ttcgccgcgg
    14161 cctctagcgg cgcccgcaac agaatggacc acacaatcaa gcccgcggcc gcgatcagga
    14221 atatgaccag ctccacgcgc agcacgtcgg gccaaaccat cacccgcagg cggtcttcgg
    14281 cttcgcaggg cggcaatccc cggcctagcc gctgatcgtt gatcgccatg cgtcgagcgg
    14341 caagccaaag gaagccgatg gtagcacaaa ccataaggga aattgcgaca ttgtcgggct
    14401 gaagaatgat ccatgagaac tgtgggtcgg cacacgccgt cgcccagccg gccaggaaga
    14461 gaaagccgag aatcgcggcg aagaccggcc ggctccacca ccggtaggcc gcgagcccga
    14521 tgaccaggac cgcgattgcg atgggcagca gcctctcggg tcggcaatac cagttgacca
    14581 atcccttgat gccgctccat gatccgaaga cggccgtgag tagcaacgcc gtcaaagctg
    14641 ccaaagcaag gacaagggct gttcgattgg agcgacggcg tgcctgcgac ggagtgtaca
    14701 gttcgtcagg cccgctgata cccccatcct tgcggattcg ccagaagtgc agcgagatga
    14761 agattgccga gatcaccggc agcacggcgc agtgcagcac gtaaaagcgg agcagggcgg
    14821 gtggtccgac gtcgcggccg ccaagaagaa gcgatttggg gtcccagccc gccggcagcc
    14881 ggccggacca gggaccgtcg ctcccgatca caggggcggc cgagaccatg ttggtcccta
    14941 ccgtgaccgc ccataaagcc aactgatccc acggcagcag atagccggtg aacgacaaca
    15001 gcatggtcag cacgagcagc acaacgccga tgcaccagtt gaactcacgg gggggtttgt
    15061 agctgcccgt cagaaacact cgcagcatgt gcagccaggc ggctatcacg atggcgtgag
    15121 cgccccagcg atgaatctcc cgcaccagac cgaaagaaac agtcgctcgc agggcctgca
    15181 tgtccgcgta ggcgtgctcg gcagccggtc ggtaatagaa catcagcgcg atgccggtga
    15241 tggtctcgat gacgaagaga acaaacgtca gccagcccat cgcccaggtg aaccagaacc
    15301 gcagcgagta ccgccgcatg tccaccggat ggatgtgcag gaaaaggttg ctgaccacga
    15361 ccagggcccg gccttggcga tcgcggggca agccgtgacg aactacggat ttccagatct
    15421 gcgatgagcg aatacggtcg ctgatgcctg ccatgggggt tgtctgtgcc ttttgcggcc
    15481 tatggccgaa ctcgatcaca gcctccgctg ccgctggcga gaacatgtag acaatctgtc
    15541 tggccgagca ttctcaactc atcattcggt attcgcaatc gtcgagtgcc ggacaaagca
    15601 ttttgaggcc gcacgcggct aagcggagat gaagctgtcc ggatggtccc attcacctcg
    15661 ttctttcctg aaccgccgat tgctgtcgat gatgacgtgg tcgtcttgaa cccggatagc
    15721 caggcgctcc aacgggcgtg gcgcaggacc gtcgaggttg gtcccgtcca ggtcgaagct
    15781 gctgccgtga caggggcagc gaaagcggcc gtcgttgctc acccacgcca cgcggcaacc
    15841 caagtgcgta caggctgcgc ggatggcgac aatcctcttt gccatccgga cgatccagat
    15901 tgccttgtca cttaccgcgt cgctgatctg acctgacgga agagcggcgt agcgatccaa
    15961 tggaccggca tcgacctcgt tctcttgccg acttgccggg ctaggccgca ggaaccgggc
    16021 ggtcgcccac gttcctgctc ccaggcttgc accgagggcg agccagccga ttccgccggc
    16081 cgagaggatc cggcgtcgcg gtacctgggg gtcattcgac atgtcgtttg ccatttcctt
    16141 gtctgcccgc cgcccttgta cttccgcacc cggaggcatg ttaccaggag atgtcggtgc
    16201 tgtcgtcggt ggttccagga atgaacggca ggcgtggatc gcggtgcggt tcagggatgc
    16261 ccgagtgcgt ccgataagac gggtgtcagc ggagtatcag ataggggacg cttcatcgtc
    16321 acaagcaagc caagcaagct tcgaatcatg ggccatgatc ggactcgtag taccgcaggt
    16381 cagagtctga tcttcaagag agataggctg gccgggggtt gaacgactgt tttcagtaga
    16441 ctctttctgc ttaagatgct tcaagatatg aacttgtgtt tattgaggtg ctggcgtggc
    16501 ggcagacccc cggcttggac ggattcgacg cggagctaga gtattcgtac cggatcaagc
    16561 ttggcgaccg acgcctggcg atccgcagac gactgtttgg ggaataattc aaccgatgtc
    16621 agattgatat tttttgcctc gtgcatgaaa aaccggtgct ttttcgcttg catacagcca
    16681 gtcgtcaggg tatgattatg cgaaggaggc tggagtgcaa caccctatct caaggaggag
    16741 tcggcagagg ctcccgcagt ggtgtgcacg taccggctga gagggaagag cgaaagagcg
    16801 agggtcagga tagcgtagtt gagcatttcg accttccgga atcttacgct cgggttgcaa
    16861 tgtgcatatc gctcacggaa tgacgaaccg cgcggttcgg ttccgcgagg cagcagcatg
    16921 acgggacagc gatcgagtcc cgttatgtcg ggtcgttttt ctgaggagga gacaagtatg
    16981 aagaaagcaa tggtatttgt gctgttggcg atggctgcgc cggccttggc gggcaacctg
    17041 aatctggccg acgcgaaagc gtggggagcg ccgtggggtc aggcggccac cgtgtcggat
    17101 cagggaaacg gcgtgttcaa cctgagcatc ggctatggta gcgctggtgt cttctggcgt
    17161 ctgcccgctt tcccgagcga gatcgtccgg atcacgggca cgtggacagg cgactgtggc
    17221 ggcgccggct gggccgaggt gatgatgttt acctcgaccg aaggcatgag cgatgccgag
    17281 atcgccaatc ggatcgatac tggcaatgcc gcggacattg ttgccaagca cgattcttgg
    17341 ggcctgaatg gcggtccggc ttggccatgg caggccattg aggctgcgcc gatgacacct
    17401 ccgggtggca aatatgagat tcacgccacc tgtgcagagg ttgtcgtggc cctgaaggtc
    17461 ggccaagtgg gccagcctgg cacatgggct acctacgatc tcaccttcat tcctgagcct
    17521 gccgcagcgt tgctgctcgg gctgccgctg atgctgctgc gtcgtcgtcg ctaatcaggc
    17581 gcgaccgagc tgaattgtca gcgattcagg caagaaacgg aggccgccgg atttgaaata
    17641 tccggcggcc tcttttgtgc gcatcgcacg cattcttcgc ggaatgcact tgcggaccgt
    17701 cgcccgcttg tgtcggcggt atggcatggg gccggccgga acgaagactc gatgcgggct
    17761 gcgtgctctg ggcggcttcc aggcctgcct tggccttctg gttgtggggg tctacctgta
    17821 aggccgtttg gtatgctttg gcggccaatt cactctgacc cttgcgaagg tacagatctc
    17881 ccagtcggca atgggcatca gcgtgcttcg gggccaatgc cacgactttg cgaaacgcgc
    17941 ttattgcctg atcggattgg tccaagccca tgtaggcgta agccaaggcg tagtaggctt
    18001 gaagctcatt gggtcgaagg gctgcggctt tctcaaaatg ggggagggcg tcgttcaagc
    18061 gattctccct ggccaggcgc tggccccacg aatagtgggt ctgggccagg cgatccgtga
    18121 cttccgggct gtcgggccga taacggctga cctcggccag acgggccagg gccttatcga
    18181 attgaccggc gtacaggtgc gccgttgcga ggttgaagag gatttcaggg gagtgtggtt
    18241 ggctctgaag tgctgcctcg aagtgccgac ttgcctcgtc ccattgtcct gccatcccgc
    18301 atgccatgcc caggccgttg tgggcctcgg ccgattcggg tttgatggcc aacgcccgag
    18361 tgaattgcga cattgcctca gtggtgtcgt tttgccggct cagagcctcg ccgtacgtga
    18421 tgcgaacctt gtagttgtcc ggatcgagct cgacggcttg ccgagcgtgc cgcacagcgt
    18481 cctcgatccg gttctgatcg atcaggtggg acgcgagcgc ctggtgagcc catgccgttt
    18541 tcggggcgat gctcaggatg tgttcgcagt acgtaccggg atcctgccac ttgagctgat
    18601 atcggtctgc ggcgacggca tagcaggccg ccgccccaag cggaacaacg acgacggcag
    18661 agcggcacag cctccttgcg acgcctgcct gggcgatgtt ccacaaccgg cttagcagcc
    18721 aggtgaggat gagaaggaag cccactgccg ggagataggc gtatttgtcg gccgccaccc
    18781 ccatggcgta ccccacgttc agcagagtag gagccagggc gacgaggaag aacagccaac
    18841 ccgccatgag tgcccgcgtc caacgcaagg atatcacaag gccgccggcg agaaggacgg
    18901 tcgagacggc cccgagaaaa acggaccggt ttgacaagtc gatgggctcg ggaaacgggt
    18961 ataccgaaca cagattcgcc ggccatatca tcttgagagg atagaacagc acgttgtggc
    19021 agacaatcag caggttttgc tcaaaggaga tgccctgcat cgcggtcaga tccacgtgtc
    19081 tttgggagat gactgtgatg atggcggaag caacggcaat cgcaaagaat ggcagcttct
    19141 cgatgagcct gcgccaatcc aatcgaccaa gtggccagta atcgagcagc aacagcagga
    19201 tcggcagggg cgtgaccgtg ggcttggaca gcagggcgag aagaaacatc accaggctgg
    19261 cggcatagat cccgcggctg cccgaacgtg cgtatcggac gtagaggacc atgctgatca
    19321 gggcgaagaa ggtggccaac ggcgtctttc gttcgcccag ccacgtgacg acctcgacgg
    19381 tcatcggatg caggccaaac agcaggcccg ccagcaccgc gggcaccggc cggtgaaaca
    19441 gcaagtagag caggatgatc accaggacgg tgttggcgac gtgcagcacc aggctcgtgc
    19501 ggtggtaggg catcaaatgg tccggccggc cgcccatggc cgagtcgagc atcagcgaga
    19561 tcatggccag cggctgatag taaccctcga ccatcgccgg tctggtcacc tccgccagga
    19621 acaggcccgc gtggcgccaa ctggggtttt gcacgcggta gttctggacg acatagtcgc
    19681 cgtcgttcat tgaaagcgct tgggccgtca acgccctcct atgggcgaag aagacgatga
    19741 ttgtgaccgc gacgagcatc accgccggca gcacccgaga cgtggtgact gcctcggttg
    19801 tatccgggcg atccgacccg gtgccgccct cgtctccagg cggtttgggc gctgatcgca
    19861 cagtgcgttt ctgttgactg cggctcatgt ccaataaggt aaccgggaac ttcgaggctg
    19921 cctacacccg tgtgttcaag gcggatggcg gccgtggggt ttcagtcggc gcagtgcgga
    19981 tccggcagcg tgtccgggcc gctcaggcat cgctggagga gcccgaaatc agcctggtcc
    20041 acgtccagat cttcatcgag cctggcatca ctgcaggcgg ggttgtattg gggtattccg
    20101 tttccggtga gacaggctag aagcgctgcc acgtcatcct gatcgacatc gcagtcgttg
    20161 tcgaggtcgc cgggcagtcg ggcgggcact tcaaactcgt acgcgcccat gtccaccacg
    20221 catccttgta tgcgaggacg ggcttcaaga tcaaccggga cgggttcaga ggcgtcgccg
    20281 tcgtggtcca gatcagcctt gtccggcggc agggcgagag tgtctccacc attgatacag
    20341 ggcgagcccg cctgtgggtg gaagccgtat atctggtagc tctcggcgcc cgtgatccag
    20401 gatgctccaa gttcaatcgt gttccgctca gctagaacgg tgacggtgtt ggcggtgttg
    20461 gcgacgatca gcatctgaag cggctgcgtc gtgtcggggt taaccagttt cccgacccat
    20521 tggttcggcg tccatgtggc gtttgcgtcc gtaaaggtga tttcgaacgt gtccggatcg
    20581 tacgttccgg gagccgtcca aattccggat gttccaccca tgaacagcgg atcggcgtcg
    20641 atgttgccgt tctgccagct taacgtgcta ccgctttcga cgtaggcccc ggcctgcccg
    20701 ttgtggatat cgctgtacga gacggtcaga ctcccaccgg cgaagacggc gatttcgggg
    20761 cctcgtgcag cagtgttgtc ccataggatg cagttgactg tcgtcgcgct gctctggttg
    20821 caggccagcg cgcccccgtc gccgccggcc atattggccg atacggtgca gtttgtgaac
    20881 acgggattgg caaggtaacc ctgtatcccc ccgccgttgg cgggagcgaa attgccgctg
    20941 acgaggcaat tggtgacggc gggggcattg gtgctgcagt agagaccgcc gccggaggtt
    21001 acgcacgtgt ttccgctgac gatgcagttg gcgatgactg ggctgttgta atagcagtac
    21061 attccgccgc cgaagtaaga gtggtttccc accagcgagc atccggagat gacggcctcg
    21121 ctcctgttac agtaaatgcc gccgcccgtg ttgccgtgat tcccgctgat gttgcagttg
    21181 gtaatgatcg ggttgctttg gtagcagcgg atgcccccgc cgctgaccgc gcggttgccg
    21241 cgaaaatcgc aaccatcgat gattgcatga ctggcgttgc agcaactgat cccgcctccg
    21301 ctgccgtagt ccgcgatgtt tccgtagaag cggcagtcaa cgatcgtcgg gcttgccgcg
    21361 tcacaggcga tgccgcctcc gaagcccgcg gtgcagttgc ggatcacgca gcctttaatc
    21421 gtcgggctgc tgccgacaca caggatggcg cccccgtcct ggccggcggc caggacggaa
    21481 ccgttgatca tggtgattcc gtcgacgatg gacgaggcac cttcgccgga atggaagcga
    21541 aacgctcgcc cggccccttc gcagtcgatg atcgtttgag cggggccgtt ctggctgcgt
    21601 acggtgatcg ctttgccgcc gaaatcgagg tcgcgattgc ccgggccggt gtagaccccg
    21661 tcgcagatgc tgatcacatc gccgggaacg gcggcgtcga tggcggcctg tatggtcgag
    21721 aagtcgcctc cgccgccctg gcagacggtg atcgtgaggc tggccgatgc cggtccgatc
    21781 tcgtacacgt atgccgagcc cgaatgtgtt cctgaatcgc tgtcatacgg cgagccgacg
    21841 atggcgaagt ctccgttcac gcaaaccgcc tgtccaaatt ggtcgcctgc ggcggcgtcc
    21901 gaggcgatga tcttggtctt ccgtatccaa gccgacgcgc gccgatggaa cacgcaggct
    21961 gcaccggcgt tgttcccgcc ttcgtcgtcg tacagtgtcc cgacgacgat ggaatcgccg
    22021 ctgatcgaga cactatggcc gaacatggca ccggtctcgg cattcgccgc ggagagcttc
    22081 tcggcctggg cccattgtgt tccctgtcgc tcgaatacgt atgccgaacc cgaatcgttc
    22141 ccgccgtcgt cggtccacgg ggccccgacg atggcgtggt caccgctgat cgatacggat
    22201 agcccgaaac cggcgaaggc ctgaggatca gccgccacga gcttggcctg ctgtgtccag
    22261 ttgtcgccgt catgaaagaa gacgtaggcg gatccggagc ccgtcaccgt atcgtcgtcg
    22321 ccgtacgcgc cgatgacggc gtggttgccg caaatactca ctgcccagcc gaagctgtcg
    22381 ccggcggcgc cgtcgtcggg cttcagcttg gcttgctgag tccacgtatc cccatttcgc
    22441 aggaagacgt aagctgatcc ggacgcgctg ccgttgtcgt cgtcgccgta cgcgccgacc
    22501 agtgcgaaat cgccggctat ggcgaccgaa ctgccgaact tgtcgccggc ggcggcgtcc
    22561 gaggcgacga gtttcgcctg ctctgtccaa cttgcgtcag ttctcgtgaa gatgtacgcg
    22621 gccccggaat ccgcacctgc gtcgtctttg tcgggggccc cgacaatgat gtagtcgccg
    22681 ctgatcgcca cgcaacggcc gaacttgtcg tccgcgccgg cgtcggaggc gtaaagcttg
    22741 gcctggtgga cccatttgag cccttcgcgg cggagaacgt aggccgaccc ggagttggag
    22801 ccgtagtcgt cgtcg
//
LOCUS       DAOTPW010000069        22766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2333_length_22766_cov_5.899696, whole genome shotgun sequence.
ACCESSION   DAOTPW010000069 DAOTPW010000000
VERSION     DAOTPW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22766
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_22766_cov_5.899696"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..886
                     /locus_tag="P5316_08195"
     CDS             200..886
                     /locus_tag="P5316_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRR84975.1"
                     /translation="MIRTQAHACYCAMAIGVSVLGGPGVSTTRPQDRKPLPPADLSGK
                     MPLEQTIATRRSIREFAPRPLTPEQIGQLCWAGQGITDRSSGYRAAPSAGALYPVELY
                     LVTREGIDHYLPRDHALMPHQAGDVRRELQRASIDQDCVGDAPLTIVIAAVVDRSARK
                     YRGRAERYCFIEAGHVAQNILLQATALGLSGVPVGAFEDERVAQVLQLPKDARVLYLL
                     PIGYPPGGER"
     gene            complement(904..3600)
                     /gene="acnA"
                     /locus_tag="P5316_08200"
     CDS             complement(904..3600)
                     /gene="acnA"
                     /locus_tag="P5316_08200"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRR84976.1"
                     /translation="MAALRDDPFGVESTLVTQAGAVRFYSLSKLAACGTGKLDQLPFS
                     IRVLLENVLRNVDGFQVTAEDVTRLAAWSPKPAEAEVPFKPGRVILQDFTGVPALVDL
                     AAMRAALKRLGGDPRRINPEVPCDLIIDHSVQVDAFGRVEALRINQEIEFERNRERYA
                     FLKWGQQAFDNFRVVPPATGIIHQVNLEYLAKVVLTRPAGGHTLAFPDSVVGTDSHTT
                     MINGLGVVGWGVGGIEAEAVMLGQPIYMLSPEVIGFRLSGQLPEGATATDLVLTVTEM
                     LRAKGVVGKFVEFFGPGLAEMSPADRATLANMSPEYGATMGFFPVDEQTLWYLAMTGR
                     PVELIDLVERYCKEQGLFRGPNTPDPVYSDTLELDMSTVEPCLAGPRRPQDRVPLKVM
                     KKQWRKDLTASYKIPDVATEKGVDTEWSGWRFKLNHGAVVIASITSCTNTSNPSVMLG
                     AGLLARRAVEKGLACKPWVKTSLAPGSRVVTDYLIKAGLMPSLERLGFHVVGYGCTTC
                     IGNSGPLPEPIADAITKGDLVVASVLSGNRNFEGRVNPLTKANYLASPLLVVAYALAG
                     TTDIDLTCEPIGKGSDGRDVYLKDIWPGEKEIREAVERCVSPEQFSLCYARVFEGDEL
                     WRSVPCGGSELYDWDVNSTYIQEVPFFTEMATEAGSVQPITGARCLVMVGDSVTTDHI
                     SPAGSIKPTSPAGLYLQSLGVKPADFNSYGARRGNDRVMVRGTFANIRLRNLLAPGTE
                     GCTTVYLPTGEQMSIYDAALKYRETGTPLIVLAGKEYGTGSSRDWAAKGPMLLGIRAV
                     IAESFERIHRSNLVGMGILPLQYKPGRNRESLGLMGTETFDILGLSNDLKPRQDVKVV
                     ARRSDGAVVEFTTICRIDTPPEIEYYRNGGILQTVIRRMLRG"
     gene            5151..7568
                     /locus_tag="P5316_08205"
     CDS             5151..7568
                     /locus_tag="P5316_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR84977.1"
                     /translation="MSEVIRHSSLAKQLATRWQVPLLIVSLALLAVGVWRLRPGPVPP
                     TFEQLLSDVRALADSGLYPEASRYASELLAAPDRTAAERRRLYAELARIIFLHEQGNA
                     VHGTGNCERIIINTNMSLGEGESFDGPTHRMRAMALEWLQKPAAALAEYQQAVEKGVP
                     DAWEIRKRIIEIRRATQGMSAQELHDLFDQFMVGDGVSQELQFWAALQKVELFGREGN
                     HAAAERFLSDHVARFRDSVFAGQFDYLQALSWYHLGRKDDAERLLRSVRNRLAASDPT
                     YICAGWLLGRILQEQESPEFALSFYEDVLDKAPPGPYRTAAILGRAETLAALERFDES
                     IESYSDAVQKTMDSPYGSLVNIQQIRESATGWYQNLLSRGRPAEAMLYLRQAARLVPP
                     ADTERQAGYAEWLADLAMKLGESLLVEEEASATAAGRVARAREYLQEAAREYLRLARL
                     ASLNEVRSSHAIWRAAEALDRAGDPRQAAEVLDRFVTDRPTNPRVPEALRRLGQMYQS
                     VGDYDRAIARYQENMVKYSGTPAAVSSLVPLADCFIEKGDLEKAEQALLRLVGHKSEE
                     SLRSVMPEAREYREALFRLGDLYIRAGKYEEAIARFQEALDRYPNDPRSGRAMFMLAE
                     SHRISADRLYDDYGDPRNAPHKDRLRATYVERLNKARRLYDGLIERFRARDASTVGLL
                     DRAYVKLSHFYRADVVHDLARVSDPSDLRPFVESLELYDRAAWLYQDDPLAMSAYIQM
                     INCYIRLGKIDKARMTLQRARWALKGISDEAFTKYSPDEDREYWTAYLDWLEKTPTFS
                     VVLAGTP"
     gene            7568..8089
                     /locus_tag="P5316_08210"
     CDS             7568..8089
                     /locus_tag="P5316_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84978.1"
                     /translation="MRMTGNLPGKETSMNVTGSTPEAKSDERDDVLEILTEQCALCRQ
                     LKGLADRQRMLIASDQAEMLLEVLGRRRQIIDRLAALANRMRPYQQCWTEVRSRMADD
                     DARRADEMISELNAALAGILQGDKADAEILAGRRRETASDLGRLKATRMAGAAYAASQ
                     EGPQVSQVEWTDQ"
     gene            8086..8988
                     /locus_tag="P5316_08215"
     CDS             8086..8988
                     /locus_tag="P5316_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR84979.1"
                     /translation="MSVVTPQITDREKELAAIIAAYNDVTDQLKRAHDKLGQEVRHLR
                     RELARKNAELRRRERLAALGEMAAGVAHEIRNPLSGIRLYASLLCRDLRDRPAELRTV
                     EKISKCVGALEEIVSDILHFGRPTTPNPAPVRLGIVVRQAVELAASRMREAAIAFEVD
                     DEVDGFELVTDAALLQRALTNLLINAAEASAGLPAREPHVRVRADRNTTDRLAVRIED
                     NGPGIPPELMDRIFNPFFTTKHNGTGLGLAIVHQIAETLGGSVRAANRPEGGAVFTLL
                     IPVRYEESPLPEEFAFGQPREEVL"
     gene            8988..10397
                     /locus_tag="P5316_08220"
     CDS             8988..10397
                     /locus_tag="P5316_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR84980.1"
                     /translation="MGRVCVVDDKEVLRESIAETLSRDDHVVTTFGDPIEALEAIKSG
                     SFDCVITDLKMPGMDGVTLLREMRAAECDASVIVMTAFGTIESAVEAMKLGALDYIQK
                     PFEAEQLSLLVDRAIQHARLRSENEALKRSMTDGETREMIGDSPAMRVVRETIARIAP
                     SNNTVLIQGESGTGKELVARRLHGASGRAGRPMLCLNCAALSSNLLESELFGHERGAF
                     TGADRARKGRFELADGGTLLLDEVSEIPLPLQAKLLRVLQERQFERVGSSTTRTVDVR
                     VIATTNRNLPDWVARKRFREDLYFRLSVLPLTIPPLRERRDDIPMLVNYFLTRAASRA
                     GHPVLRVSDGAMRLLTDYDWPGNVRELENLCERASVLVLDGVLTEQIIVPWLNGIQRA
                     ESFPEKLRHGHIIEDMERLLIEKTLRECAGHRARTAEALGIGVRTLTLKLKQWREQDE
                     ARRSLLAAQSSGAMLVGSK"
     gene            10627..11061
                     /gene="flgB"
                     /locus_tag="P5316_08225"
     CDS             10627..11061
                     /gene="flgB"
                     /locus_tag="P5316_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRR84981.1"
                     /translation="MLFHGAVNSGTVPVLEKVLAYTHARHRMLTENIANIDTPGYKAR
                     QLNVRAFQNALKEAIARRPSERGPLSLRGAREFREDASGCLVVTPTEEPAENVLFHDR
                     TNMRIERQMAMLAENTLMHQIASDRLKGRFDQLMTAIRGTVA"
     gene            11142..11552
                     /gene="flgC"
                     /locus_tag="P5316_08230"
     CDS             11142..11552
                     /gene="flgC"
                     /locus_tag="P5316_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRR84982.1"
                     /translation="MFGSLDVSTSALVAQRIRMDTIHGNIANAQATQRADGRPGPYKR
                     RIAVFQTGDGAGRPGVHVSKIVEDPTVGQRVYEPEHPHAIKSGPYQGYVEYPNVDLPT
                     EMVNAILATRAYEANITAMEATKNMISASLRILA"
     gene            11599..11916
                     /gene="fliE"
                     /locus_tag="P5316_08235"
     CDS             11599..11916
                     /gene="fliE"
                     /locus_tag="P5316_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRR84983.1"
                     /translation="MPDPLSIHGIGAGQPPGPLGRTLSTGAGRETGGPSFREVLLNSL
                     EQVNRLQQEANEGVEKIMTGQTDNLAAVFSAVRKSEVAFSMLMEMRNKLIDAYREIQQ
                     MRV"
     gene            12020..13660
                     /locus_tag="P5316_08240"
     CDS             12020..13660
                     /locus_tag="P5316_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HRR84984.1"
                     /translation="MEFLRKIALQTKVHLQGLTTSQKLAIVLSVILIAVAVLALVRWA
                     AEPAMVILLDQPLTPEASARIQQFLDAAGVKYELAGDVVRVPIDQRPRLLAQLTQQKL
                     LPDDISLGFAKLIGEASNPWLPMQEQDRRWNVARCNELARVLREFNGIRDARVFVETA
                     TRRHVGQPNAQPTASVSVKLADGATLDKELVRAIAGFVSSAVSNLSIYNVTVADATTG
                     RAAGVPRPEDGMDADDIDRRKAKEEYFANKIRELLSDIPVSVKVYCELEPESRQEIKE
                     VYGKPVIATESTTSETDTTSTMAGEPGVVPNTTVSVAGGAPVNNHEKTTSDMTYKSEA
                     DKTVTQTKRPRDTIKSISASVTIPKSYLLNVFNLPKETSDQELDTASTSLREKIKKSV
                     MTALAIPPDAEERVTVDWFPDAGALALAHLGSSGAGEGVMSMVSTHWDKAGMAVLAVL
                     GLLMMLMMVRKVGEAPVLPGEDSPKAGRKGKLPVAEVEEEVPVSEAKETEPLLVGKEV
                     DEETLRARQIVEQVGELIKGDPVSSASILQRWIEEANA"
     gene            13663..14709
                     /gene="fliG"
                     /locus_tag="P5316_08245"
     CDS             13663..14709
                     /gene="fliG"
                     /locus_tag="P5316_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRR84985.1"
                     /translation="MAKKGKNKENEQGAEKGFTGIRKVAAFLLAIGPEAAGSLLRRMD
                     REIVEDVTREVASLGVVPPEQQKAIVEEFYNVALARQYANEGGLSYARALLERALSKE
                     EAARVMQQIEHQVHQQPFSFLQKAESEHLLTFMQDEHPQTIALILAHLPSSKGSEILS
                     GLPPAKQIEVVTRVANMEQTNPEVIREVEKGLEQRLSGVMTQQFQKVGGVEAVAEMLN
                     LSDRATEKGILETLEAEDPELVEQIRRLMFVFEDILLVNDKGIQALLKEIDNDELALA
                     LKTASDEMKEKIFSNMSERAAQLIKEDMEYMGPVRLSDVEAAQQRIVDVVRRLEDAGE
                     IIISGRGGESDVVV"
     gene            14734..15504
                     /locus_tag="P5316_08250"
     CDS             14734..15504
                     /locus_tag="P5316_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HRR84986.1"
                     /translation="MARRSTVIKAAEALPFQGAVLKPFHLSDMMGEVKAALIQAQADA
                     ARIVRQAKAQEDAIRRAGYEAGYQAGFAKGLEEGREKGHAQAFAEAKAQFAATHKSLL
                     SSCEKIIADIEERRAAWQAAARQDLVDLAMAVARRIVRHVSENDRRVVVENLEEAIRL
                     AGERSDVTIKINPADAQAVRVFAESLADRQASCKLVKIVEAPEISPGGCWVQWGSGAV
                     DARIETQLDRIAAELGVRGTPSIQHAAENMTREGPENA"
     gene            15501..16871
                     /locus_tag="P5316_08255"
     CDS             15501..16871
                     /locus_tag="P5316_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014970684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HRR84987.1"
                     /translation="MNAFAKEIEMVDSMVTPSLVGRVVDVTGLTITVSGLPAPIGAMC
                     TIERQNGTRIEAQVVGFRNDHTIVMPLQDTLGVARGDNVTAWPGEAKVPLCDEMIGRV
                     MDGLGRVIDNGPPLYCDTCYPVYRPAPRALERPRISHPLSTGIRSIDALLTAGRGQRL
                     GLFSGTGVGKSVLLGMIARYTDADVTVLALIGERGREVNEFLQNDLGPEGRRKTVLVL
                     STSDESPVLRVRAGFVATAVAEYFRDRGKNVLLLMDSITRIAMAQRQIGLAAGEPPAT
                     KGYTPSAFALLPQLLERAGQTENGSITGFYTVLVEADDINDPVGDAVRGILDGHVWLS
                     RALANKSHYPAVSVSESISRVMSSIVDPQHLAAARTVMKAVAIWNEIEDLVNIGAYAA
                     GANAEYDAVIQTRPAVLAFLQQGVAQRVSFAEARQQLLALAQQIEETTRRLSREGGRA
                     RTVTAA"
     gene            16888..17364
                     /locus_tag="P5316_08260"
     CDS             16888..17364
                     /locus_tag="P5316_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HRR84988.1"
                     /translation="MPRRFKFRFATMLKIRRQREDEQKRIVAERLRQIRQTRDHRLVL
                     QRQIEDEVDAIRHSQTGGAIDIQQVMRHRHWLSHLHKGVLEADARLRFLEARLAQERV
                     VLAEAVKQRKILEKLKERQWQRHLREQGVLEVKEGDELAAVRYVFGRDADAELLRT"
     gene            17409..18086
                     /locus_tag="P5316_08265"
     CDS             17409..18086
                     /locus_tag="P5316_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84989.1"
                     /translation="MLKRFYHLLALLALINLFAVAGFVGYLFASGRLDAERARQIAVV
                     LRGEFPASQPAAAAGTPTPRDESPQASGDEIARQQQTKELLALQERRRARDLRDRDLL
                     DKRIQLETRQLLEKLERKENELKQQKQVFEKEGEQAGFDRQLNLLSKTEPKLALQLLK
                     NQIKEPDAVQLLMKMDENRVKGIINACKTEEDKAWIGRILNQIGKYGSSTAAGGDDSG
                     KPASDGG"
     gene            18088..19842
                     /locus_tag="P5316_08270"
     CDS             18088..19842
                     /locus_tag="P5316_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRR84990.1"
                     /translation="MPTVEWAEVRTVAHSAQTVRDKPEQLDDVFAVVFDSARRSVGSR
                     VDRTVREQQHRAADKDKAAATRDPQAVTSGRSKSKGAEGPQEPKKNQQTAKPESPVKG
                     TPIEPAQAESPQPAPAEESPRANEPLVVEHATEPSAADPVPQVPPELKPAPSQKSSEQ
                     TKAAQNLLWKPTPGATEFPMDTAPAENRVDDSPTTQTSPQLVIWAVADPVAAVIPDIP
                     ATAQAGDAAPGGWAPPQATEVSATRETTGTSSGEQAPQPASGANVQPAQELSISVWQF
                     LRGVAAHLEQAIETRDRRAMNKQPASCSEPTVAARPTGETPPNPMTKPPLLGSLENGS
                     STNKQLLGQDENRSPEHDAASQASSGDAARNADGSAHQTDTNDEFRGLLELAGRVRGR
                     IVSPDLQPAPASFGRDLGGQGISLKEARAMTELADVVRANIGARHSSMVLKLEPPELG
                     RLRVDVRTHGDELTLRLQADSLLGRDALQSRINELRSSLEQHGFKLNQIQVELRVPQA
                     NPGEAHQDRPFQQQEHSDGQSSHEPAWQGHEGGGSQTPSESEWSNEAFAFSQENASSG
                     EEQDRVDRLAETGVDLVV"
     gene            19932..20429
                     /locus_tag="P5316_08275"
     CDS             19932..20429
                     /locus_tag="P5316_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRR84991.1"
                     /translation="MQITATRYTDGSTAQTTTQRDVSGISGQDFMKILIKQLQLQDPF
                     EPMTNQEMISQMATIRELEMNTRLTERLEQLTDQQRVASASGLIGKQIKGIVTDSNGN
                     EYELKGLVTGIEFTSRGEILLRLDNGLRLPITSLTEVTEPRSDLETEEDSESASQEEK
                     SEAAA"
     gene            20546..22258
                     /locus_tag="P5316_08280"
     CDS             20546..22258
                     /locus_tag="P5316_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HRR84992.1"
                     /translation="MGLTSAMYTGLSGLNANQYRIDTVGNNIANVNTTAFKGSRASFQ
                     NQFSVMLSGGSAPSNTSGGTNPSQVGMGSLVGSIQRNFQSGALETTGVPTDMAIEGNG
                     FFIVRTPELRQAYTRDGMFALDASNTLVTSDGYPVQGYGIDAAFNIDRSRLVDLNIPL
                     GTLTIARATSSAAFDGNLNANGTIGTQGTILYSQVFTDGSCGPAATGATLLTNLYDPT
                     VSTTSALFAVGDVITLQDVRKGGRQIETASFTVDAASTLDDFLTFLNNELGLNADPAV
                     PNTPGITVASGLGTPPDGTIIIEGNVGYDNRIEIAQVGIRNSNETVTASPFQFTPQQQ
                     ANGESVYTSFLVYDSLGTPIQVSVTAVLESKTDGGTLWRFYTESTDDTDPTPVLGATG
                     TIQFDNNGRLLNVTNNILQIDRDNSGASNPVSFELDFSNLTGLTTDMSTLVMTFQDGF
                     EAGTLTSFNTGADGVINGVFTNGLSRPLGQLALATFINCQGLVSRTDNLYYEGSNSGQ
                     PLITAPGNLGAGTIQGATLELSNVDLTREFIGLVTASTGFSASGRVINTSNELLNELL
                     SIAR"
     gene            22348..22539
                     /locus_tag="P5316_08285"
     CDS             22348..22539
                     /locus_tag="P5316_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HRR84993.1"
                     /translation="MITLTRLNGTRFVINAELIRTVEERPDTTIVLINGETFIVRERM
                     EEVVARAIEYGRMIRVFRP"
     gene            complement(22529..>22766)
                     /locus_tag="P5316_08290"
     CDS             complement(22529..>22766)
                     /locus_tag="P5316_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84994.1"
                     /translation="VNTSPTAVARSTMATSCVPRRGTYRTIRASEYLVSYIGHAVHVI
                     HLPGEVSQAGGHGAGGALRESSESSTGLPAATTV"
BASE COUNT         4683 a         7350 c         6819 g         3914 t
ORIGIN      
        1 gcagcgcaga cccggccgac gagggccgtt accgccccga cgtgttgccc gatgtcgaag
       61 gcgggcatcg gcaaacgcga cgggatcgaa tagcttctgc cattcatcga ccagctcgac
      121 gcacggcccg gttacaatgc ggcgcccggg accgtaggat gtccggaaaa cgctcccgtc
      181 ctgttacgcg gaggatacaa tgatcagaac acaagctcat gcttgctact gcgcgatggc
      241 tatcggtgtg tcagttctgg gaggaccagg cgtgtccacc acaaggccgc aagaccgcaa
      301 accattgccc cccgcggatc tgtccggcaa gatgcccctc gaacagacca tcgcaacgcg
      361 acgttccatt cgagagtttg cccccaggcc cctgacgccc gagcagatcg gccaactctg
      421 ctgggccggt caaggcatca ccgaccgctc ctccggctac cgcgccgctc cgtcggccgg
      481 ggccctgtat cccgtcgagc tctacctggt gacccgcgaa ggcattgacc attacctgcc
      541 tcgcgaccat gcactcatgc cgcaccaggc cggcgacgtt cgccgtgaac tccaacgggc
      601 cagcatcgat caggactgtg tgggcgatgc ccccttgacg atcgttatcg ccgccgtcgt
      661 tgatcgctcg gcccgcaagt accgaggccg ggccgaacgc tactgcttca tcgaggccgg
      721 acatgtggcc cagaatatcc tcctccaggc gaccgccctc ggcctgagcg gcgtaccagt
      781 cggggcgttc gaggatgagc gggtggccca agtccttcaa cttcccaagg acgcccgcgt
      841 cctctatctg ttgccaatag gttacccgcc cggcggggaa cgatgatcgc cggttgaaat
      901 gactcaaccc cgcagcatcc tgcgaatcac cgtctgtaag atcccgccgt tgcggtagta
      961 ttcgatctcc ggcggcgtgt cgatccgaca aatggtcgtg aactctacga ccgcgccgtc
     1021 gctgcggcgg gcaaccacct tcacatcctg tcgcggttta aggtcattgc tcagcccaag
     1081 aatgtcgaac gtctccgtgc ccatcaggcc gagtgattcg cggtttcggc ccggcttgta
     1141 ttgcagtggc aggatgccca ttcccaccag gttgctgcgg tgaatccgct cgaaactctc
     1201 ggcgatcacc gcccggatgc ccagcaacat cggccccttg gccgcccagt cccgcgatga
     1261 gccggtaccg tattccttgc cggccagaac gatcagtggc gtcccggtct ctctgtactt
     1321 caaggccgca tcatagatgc tcatctgctc gcccgttggc aggtagacgg ttgtgcagcc
     1381 ttccgtgccg ggcgccagca ggttccgcag gcgaatgttg gcgaacgtgc cgcggaccat
     1441 cacccggtca ttccctcgcc gggcaccata actgttgaaa tcggccggtt tgaccccaag
     1501 cgactgcaaa taaagcccgg ctggacttgt cggcttgatg gaaccggccg gagaaatgtg
     1561 atccgtggtt accgaatcgc ccaccatcac cagacagcgg gcaccggtaa tcggttgaac
     1621 cgaccccgct tccgtggcca tctccgtgaa gaacggcact tcttggatgt aagtgctgtt
     1681 gacgtcccaa tcgtatagct cgctcccgcc gcaaggcacc gatcgccaca actcatcgcc
     1741 ttcgaacacc ctggcgtaac agaggctgaa ctgctccggg ctcacgcatc gctcgacggc
     1801 ctccctgatc tccttctcgc ccggccagat gtccttgaga tacacgtcac gtccgtcgga
     1861 gcccttcccg atcggctcgc aggtcaaatc gatgtccgtc gttccggcca gagcatacgc
     1921 caccaccagc aacgggctgg ccagatagtt ggccttggtc agcggattga cccgcccctc
     1981 gaaattgcgg ttgccgctca gaaccgaagc caccaccaga tcgcctttag ttatcgcatc
     2041 agcgattggc tccggcagcg gcccgctgtt gccgatgcag gtggtacagc cataacccac
     2101 gacgtggaac ccgagtctct ccagtgacgg catcaggccg gctttgatca ggtaatcagt
     2161 gaccacccgt gagccgggcg ccaggctggt cttgacccac ggcttgcaag ccagtccctt
     2221 ctcaaccgct ctcctggcca gcagaccggc cccgagcatc accgacggat tagacgtgtt
     2281 ggtgcaactg gtaattgaag cgatcacgac cgccccgtgg ttgagcttga agcgccagcc
     2341 gctccactca gtgtcgacgc ccttctccgt cgcgacatcg gggatcttgt aggacgcagt
     2401 caagtctttg cgccattgct tcttcatcac cttcagcgga acccggtcct ggggccgccg
     2461 cggacccgcc agacacggtt ccaccgtgct catgtcgagt tcaagcgtat cgctataaac
     2521 cggatcgggt gtgttcggcc ccctgaacag cccctgttcc ttgcagtaac gctcgaccag
     2581 gtcaatcaac tccaccggtc gacccgtcat ggccagatac cacagtgtct gctcgtcaac
     2641 cggaaagaac cccatcgtcg cgccatattc cggactcatg ttggccaatg tggcacggtc
     2701 tgccggcgac atctccgcca agccggggcc gaaaaactcg acgaacttgc cgaccactcc
     2761 cttggctctc agcatctcag tgaccgtcag cacaagatcc gtggccgtcg ccccctccgg
     2821 gagctggccg gacagtctga atccgatcac ctcagggctg agcatataga tcggctgccc
     2881 gagcatgacc gcctcggcct cgattccccc aacaccccag cccaccaccc ccagcccgtt
     2941 gatcatggtg gtgtggctgt ctgtcccgac aacgctgtcc ggaaacgcca gagtgtgtcc
     3001 ccctgcgggg cgcgtgagga caactttggc taggtactca agattcacct gatggatgat
     3061 gccggtcgcc ggcggcacca cgcggaagtt gtcgaaggcc tgttgccccc atttcagaaa
     3121 ggcataccgc tcgcggttcc gctcaaactc gatttcctgg ttgatgcgca gggcctcgac
     3181 cctgccgaac gcgtccacct gaaccgaatg atcgatgatc aggtcacagg gaacctccgg
     3241 attgatcctt cgaggatccc cgcccaggcg cttcaaggcc gcccgcatcg ccgccaagtc
     3301 gactaaggcg ggtacgccgg tgaagtcctg gagaatcacc ctgcccggct tgaagggtac
     3361 ttcagcctcg gccggcttgg gactccatgc tgccaatcgg gtcacatcct ccgctgtcac
     3421 ctggaaacca tccacgttgc gcaggacgtt ctcaagaaga acccggatcg aaaacgggag
     3481 ctgatcgagc ttaccagttc cgcaggccgc cagcttggac aggctgtaga aacgcacagc
     3541 ccccgcctga gtgaccagcg tcgactcaac cccgaacggg tcgtctctca gtgctgccat
     3601 aagtgcctct gttgacgtat cttgcgtctg tcctgttctc gccgcatgcc catctaccgc
     3661 cgagcacgcc tccgacatcg cctgtcgaaa aaccgccgca atcagcaggc agcacagaag
     3721 atagcgattc tctgtctgcc ttgccacctc ggtggccggg gcgctgcggg aatatgccga
     3781 gacgaaaacc cgtctctcgc gcgactataa acaaccgcga tagttgtaat tgcctgcaac
     3841 gcaaatcgcg ggcatgtttc taatcatatg gcagcgtccc tttgggagca tgcgtgctac
     3901 ggtgcgtcgc gaggttgtgg ccggcatggt gtggcagacg agtgcggtgc ttgggcaatc
     3961 tgggtaatag aaccctacgg cgagacagcg gtgcggcttg cgcgcatgtt tccggatgcc
     4021 agtgcgcttg ggttgcggcc cgcaaaaagg cggtccgcct tcggttgagg catgggtgct
     4081 tgggaggcag tcttgtgcct gctggatgcg gggttggtcc gaatgaatgc aatggcaaac
     4141 cggggtgttg ccgttgctcg ccgggccgcc ccgcaaccgg ttcgacgtgc ggaaggcaga
     4201 aatgaaggca tcgccgacag gcttgcagtg cttggttgtg ggtttgggct gcgtgctctc
     4261 aaccgtgctt ctcacatcgc ctcagagact gcctgctccc tttcggacag gcatacggac
     4321 ggcggttgat gcagtagccg gtggcctgtt gtggctgtgg ggacagcctt ccgaagtgtg
     4381 gcagcagcct gatgagtacc gtgaccacgg tgaccaggct gatgaccgtc gcctggacgc
     4441 tttcttcgac acatccccga acagagttac cccacccatg agtgaaggcc tcggcaacca
     4501 cacgactctc tgaacctctg cgactcgaac acgcaacacg atcgtcgctg ttgttgcgga
     4561 ggcagcttac ctccaagcga cgctgaaaag cacatgtccc ttttagaacc tcacggccag
     4621 atatgtgtgc gacttgcgcg catgtttgtg gatcgcagag cgcttgggtg gtggcccgca
     4681 aaaaggcgct ccgccttaga accctatggc cagatatgtg tgcggcttgc gcgcatgttt
     4741 ttggatcgca gagcgcttgg gtggtggccc gcaaaaaggc gctccgcctt cgatcaatcc
     4801 gagttccgtt gccgccgaat agttcaccgg actgagcagc gcaagttatc ggacattttg
     4861 ttgatgatct cggcaaaatc tttcttcctt cccagccacc ttgcgcgtct gtgttgtcaa
     4921 tactgccacg tgggatgtca tcgtccggta aggctgctaa cgcggttcga ccctcgctga
     4981 tcttgcgcgc ggatcggcaa aaaatgtctt caccagccgg ttcgccgctg ttctaaacgc
     5041 cccagaactg ccgactaaga tcaaactcga tctcaggtct caatcttgac ccgagcatcg
     5101 gtcgttgaaa ggggcggtca ggaagacccc tgttggttgg aaggctcagg atgagcgaag
     5161 tcattcggca cagcagcctg gcgaagcagt tggcgacgcg atggcaggtt ccgttgctga
     5221 tcgtgtcgct cgcgctgttg gcggtcggag tctggcgcct ccgtcccgga ccggttcctc
     5281 ctaccttcga acaactgctg agcgatgtga gagctttggc cgactcaggc ctctatcccg
     5341 aggccagccg gtatgcctct gagctgctgg ctgctcccga ccgcacggcc gccgagagac
     5401 gccggttata cgccgagctt gccaggatca tctttctcca cgaacagggc aatgccgttc
     5461 acggtacggg caattgcgag cggatcatca tcaatacaaa catgtccctc ggcgagggag
     5521 aatcgttcga cggaccgacg catcgaatgc gcgccatggc cctcgagtgg ctccagaagc
     5581 cggccgccgc cttggccgag taccagcaag cagtcgagaa aggcgtcccc gacgcttggg
     5641 agatccgcaa acgcattatc gagatccgcc gagccaccca gggcatgagc gcccaggaat
     5701 tgcatgacct gttcgaccag ttcatggtcg gcgatggagt gagtcaggaa cttcaattct
     5761 gggcggcgct tcaaaaggtc gagctcttcg gacgcgaagg aaaccacgcg gctgccgagc
     5821 gcttcctctc ggaccatgtc gctcggttcc gcgactcggt gtttgccggt caattcgact
     5881 atcttcaggc cctctcatgg tatcacctcg gccgcaagga tgacgcagag cggctgctcc
     5941 gctcggttcg caaccgcctg gccgcttccg atcccactta tatctgcgcc ggctggttgc
     6001 ttggcagaat cctccaggaa caggagtcgc ccgagtttgc gctctcgttt tacgaagacg
     6061 tgctcgacaa ggctccgccg ggtccgtatc gcacggcggc catcctcggc cgcgccgaaa
     6121 cgctggctgc cctcgaacga ttcgatgagt ctatcgaatc gtattccgac gcggttcaga
     6181 agaccatgga cagcccctac ggatcgctcg tcaatatcca acagatacgc gaatcggcca
     6241 ccggctggta tcagaacctc ctgtcccgcg ggcgcccggc cgaggccatg ctttatctcc
     6301 gccaggcggc ccggctcgtg ccccccgcgg ataccgaacg acaggccggc tacgcggaat
     6361 ggctggccga cctggccatg aaactcggag aatctctgct ggtcgaggaa gaggcttccg
     6421 ccaccgccgc cggccgcgtc gccagggccc gcgaatacct ccaagaggct gcccgcgagt
     6481 atctcaggct tgcccgactc gcctcgctca atgaggttcg gtcgtcccat gcgatctggc
     6541 gcgccgccga ggccctcgac cgggccggcg accctcgcca ggccgcggag gtcctggacc
     6601 gcttcgtcac cgaccgaccc accaacccac gggtgccgga ggccctgcgg cggcttgggc
     6661 agatgtacca gtccgtcggc gactacgacc gggctattgc ccgataccag gaaaatatgg
     6721 tcaaatactc tggaacgcct gccgcggtct cttcactcgt ccccttggcg gattgcttca
     6781 tcgaaaaggg ggacctcgag aaagccgagc aggccctctt gagactcgtg ggccacaagt
     6841 ccgaggaatc cctccgctcg gtgatgcccg aggcccgcga gtatcgtgag gccctgttcc
     6901 gcctgggcga tctctacatc cgcgccggaa agtacgagga ggctatcgct cgcttccagg
     6961 aggccctcga tcgctacccg aatgatcccc ggagcggccg ggcgatgttc atgctcgccg
     7021 agtcccatcg catcagcgcc gaccggctct acgacgacta cggcgatccc agaaacgcgc
     7081 cgcacaagga ccgcctgcgg gccacgtacg tcgagcggct caacaaggca cgccggctct
     7141 acgacggcct gattgagcga ttccgcgccc gcgatgcctc gacggtcggc ctgctcgacc
     7201 gggcctacgt caaactgagc cacttctaca gggccgatgt ggttcacgat cttgcccgcg
     7261 tctccgatcc gtcggacctg aggccctttg tcgagtccct cgaattgtat gaccgcgcgg
     7321 cgtggttgta tcaggacgat ccgttggcca tgtccgccta tatccagatg atcaactgct
     7381 acatccggtt gggaaagatc gacaaggcga gaatgacgct gcaacgggcc cgctgggcgc
     7441 tcaagggtat ctcagatgag gcgttcacca agtactcccc ggatgaggac cgcgaatact
     7501 ggacggcata cctcgactgg cttgagaaaa cgccgacctt ttcggtcgtc ctcgcgggga
     7561 ctccgtgatg cgaatgacgg gaaaccttcc cggaaaggaa acgtcgatga acgtgaccgg
     7621 ctcaacaccc gaggcgaaaa gcgatgagcg ggacgacgtg ctcgaaatcc tgaccgagca
     7681 gtgcgcgctc tgtcggcaac tcaaggggtt ggccgaccgt cagcgaatgc tgatcgcttc
     7741 ggatcaggcg gagatgcttc tcgaagtgct cggccggcgc cggcagatca tcgaccggct
     7801 ggcagcgctg gccaaccgga tgcggcctta tcagcaatgt tggaccgagg tccgctcacg
     7861 tatggccgat gacgacgccc ggcgggccga tgagatgatc tcagagctca acgccgctct
     7921 ggccggcatc ctgcagggcg acaaggccga cgccgagatc ctcgccggcc gacgccggga
     7981 gaccgcatcc gacctcggtc gcctgaaagc cacgcgaatg gcgggagcgg cctacgccgc
     8041 cagccaggaa gggccgcaag tctcgcaagt ggagtggacg gaccaatgag tgtcgtaacg
     8101 ccacagatca cggaccgcga aaaagagctg gccgccatca tcgccgccta caacgacgtg
     8161 accgaccagc tcaagcgggc gcacgacaag ctcgggcagg aggtgcgtca cctgcgccgg
     8221 gaactggccc gtaagaacgc cgagttgcgc cgccgtgagc ggcttgccgc gctcggcgag
     8281 atggccgccg gcgttgccca cgaaatccgc aatcctctca gcggcatccg cttgtatgcc
     8341 tcgctgctgt gccgcgatct ccgggaccgg cctgcggagc tgcgcaccgt cgaaaagatc
     8401 agtaagtgcg tcggcgcctt ggaggagatc gtttcggata ttctccattt cggccggccg
     8461 acgaccccca accccgctcc ggtcagactc ggaatcgtgg tgcggcaggc cgtcgaactg
     8521 gccgccagcc ggatgcggga ggcggcaata gctttcgagg tggacgacga agtcgacggc
     8581 tttgaactgg tcaccgacgc cgcattgctc cagcgggcct tgacaaacct cttgatcaac
     8641 gccgccgagg cgtcggccgg cctccccgcc cgagaaccgc acgtccgggt gcgcgcggat
     8701 cgcaacacga ccgatcgtct cgccgttcga atcgaagaca atggccccgg catccccccg
     8761 gagctcatgg atcgaatctt caatccgttt ttcaccacca agcacaacgg aacgggcctg
     8821 ggactggcta tcgtgcatca gatcgccgag accctgggag gcagcgtccg ggccgccaac
     8881 cggcccgaag gcggagccgt gttcaccctc ctcattccag tgcgatacga ggagtcgccc
     8941 ctcccggaag agttcgcttt cggtcaaccc cgcgaggagg ttctctaatg ggtcgagtct
     9001 gcgttgtgga cgacaaggaa gtattgcgag agtcgatcgc cgaaacgttg tcgcgggacg
     9061 accatgtcgt gacaaccttc ggcgatccga tcgaggccct ggaagcaatc aaaagcgggt
     9121 cgttcgattg cgtcattacc gacctcaaaa tgccgggcat ggacggcgtg acgctcctcc
     9181 gcgagatgcg ggccgccgaa tgcgacgcct cggtcatcgt catgaccgcc ttcggcacga
     9241 tcgagtccgc cgtcgaggcg atgaaactgg gagccctgga ttacatccag aagcctttcg
     9301 aggccgagca gctttcgctt ctggtggacc gcgccattca gcatgcacgc ctgcgatccg
     9361 aaaacgaagc cctcaagcgg tccatgactg acggcgaaac ccgggaaatg attggcgact
     9421 cgccggcaat gcgggtggta cgcgaaacca tcgcccggat cgcaccgagc aacaacaccg
     9481 tgcttatcca gggcgaatcg ggaaccggca aggaactggt cgcccggcga ttgcacggcg
     9541 cctccggacg ggccggccgc cccatgctgt gcctgaattg cgcggccttg tcaagcaacc
     9601 tgctggaaag cgaattgttc ggacacgaac ggggggcctt caccggcgcc gaccgggccc
     9661 gcaagggacg atttgaactg gccgatggcg gcacgctgct ccttgatgag gtcagcgaga
     9721 ttccccttcc cctccaagcc aagctgctcc gcgtgctcca ggaacgacag ttcgagcgcg
     9781 tcggcagcag caccacccga accgtggatg ttcgcgtgat cgccacaacc aatcgcaatc
     9841 ttcccgattg ggtggcccgg aaacgcttcc gcgaggacct ttacttccgc ctcagcgtcc
     9901 ttccgctgac gattccgccg ctgcgcgaac gacgtgacga cattcccatg ctggtcaatt
     9961 actttctcac gcgggccgct tcccgggcgg ggcacccggt gttgcgggta tccgacgggg
    10021 cgatgcgcct cctgaccgat tatgactggc cgggtaacgt tcgagaactc gaaaacctgt
    10081 gcgagcgggc atcggtgctg gtactcgacg gagtgctcac cgagcagatc atcgttccgt
    10141 ggctcaacgg aattcagcgc gccgagtcgt tccccgaaaa acttcgccac ggccacatca
    10201 ttgaggacat ggagcggctg ttgatcgaga aaaccttgcg ggaatgcgcc ggccatcggg
    10261 ccaggaccgc cgaggccctc ggaatcggcg tgcgaacgct gaccctcaaa ctcaaacagt
    10321 ggcgggaaca ggacgaggcc agacgatctc tgctggcggc ccagagcagc ggtgcaatgc
    10381 tcgtcggatc caagtgagga aagaccgagg tccagacgtg atgcgatcgc gaaaacaact
    10441 gcacggcaag gcggcaagaa ccgcgcgtca ggcgcagaaa ttgcgcttca gagccgccaa
    10501 attgcctgtt ttcacattcg caaaccgcct tgacccgtct ggtatcgatt ttgctgattc
    10561 aataccccgc agcgcggcaa gttcgcgccg acgcgcccga ttccgcgctc tggacaggaa
    10621 gacgtgatgc tctttcacgg tgcggtcaat agcgggacag tcccggtgct tgagaaggtg
    10681 ctggcctata cccatgccag acaccggatg ttgaccgaga acatcgccaa catcgacacc
    10741 cccgggtaca aggcccggca gctcaacgtg cgggcctttc agaacgccct gaaagaggcg
    10801 attgccaggc ggcccagtga gcgaggcccc ctgagcctcc gaggagcccg cgagttccgg
    10861 gaagacgcct caggttgcct ggtggtgact ccgaccgagg aaccggcgga gaacgtcctc
    10921 tttcatgacc gaacgaacat gaggatcgag aggcagatgg cgatgttggc cgagaacacg
    10981 ctcatgcacc agattgcctc ggatcggctc aagggccggt ttgatcaact catgaccgcg
    11041 atccggggaa cagtggcatg atccaggccc ggcgcgcggc aggcgcctcc ggcacgaagg
    11101 ccggtgtgca tcgccacaac ggcggaagat gaggtgatgg aatgtttggc tcgctcgatg
    11161 tcagcacgtc ggcgttggtc gcccagagga tccgaatgga tacgatccac ggcaacatcg
    11221 ccaacgccca ggcgacacaa cgcgccgacg gccggccggg cccctacaag cggcgaatcg
    11281 cggtttttca gaccggtgat ggcgccggcc ggcctggagt gcatgtcagc aaaatcgtcg
    11341 aggatccgac ggtgggacag cgcgtctatg agcccgaaca cccgcacgcg atcaagtccg
    11401 ggccgtacca gggctacgtc gagtatccca acgtcgatct acccaccgag atggtcaatg
    11461 cgattctggc cactcgcgcg tatgaagcca acatcaccgc catggaggcc acaaaaaaca
    11521 tgatatccgc cagcttgcgg attctcgcat gagaggatgc gacagcacag gtcgccgttt
    11581 cggtagaaag gcagacacat gcccgaccca ctcagtatcc atggaattgg cgcgggtcaa
    11641 ccgcccggcc cccttggcag gacgctctca accggtgcgg gccgcgagac cggcgggccc
    11701 tcgttccgcg aggtcctgct caatagcctc gaacaagtca accgccttca gcaagaggcc
    11761 aacgagggcg tcgagaaaat catgaccggc cagaccgaca acctggccgc cgtgttcagt
    11821 gccgtgcgaa agtccgaggt ggctttcagc atgctcatgg agatgcgaaa caagttgatc
    11881 gacgcatatc gcgagatcca gcagatgcgc gtgtaacgcg gggtgagtga tccccgccgc
    11941 gccggtcgaa atcggttgtt gcgggccgga acggcaagga ggcctcgggg cccggccgca
    12001 atgagggagg ctgacaggaa tggaattcct tagaaagatc gcgctccaga ccaaggtcca
    12061 tcttcaggga ctgacgacct cacagaagct ggcgatcgtc ttatcggtca tcctgatcgc
    12121 agtggctgtc ttggccctcg ttcgttgggc ggccgaaccg gcgatggtga tcctcctcga
    12181 tcagcccctg accccggagg cgtccgctcg tatccagcag ttcctcgatg ccgccggcgt
    12241 caaatacgaa ctggccggcg acgttgtccg cgtgcccatc gatcaacgcc ccaggctcct
    12301 ggcccagctt acccagcaga agctgctccc cgatgacatc agcctcggat tcgccaagct
    12361 gatcggcgag gccagcaatc cctggctgcc catgcaggag caggaccggc gctggaacgt
    12421 ggcccgctgt aacgagctgg cccgcgtgct cagggaattc aacggcatcc gcgatgcccg
    12481 cgtgttcgtc gagacggcca ccagacgcca tgtcggacaa cccaacgccc aaccaaccgc
    12541 cagcgtgtcc gtcaagttgg ccgacggcgc gactctggac aaggaactcg tccgggcgat
    12601 cgccggcttc gtcagctcgg ccgtgtccaa cctcagcata tacaacgtca ccgtggccga
    12661 cgccaccacc ggccgcgcag ccggcgtgcc aagacccgag gacggaatgg atgccgatga
    12721 catcgatcgg cggaaggcga aggaggagta ctttgctaac aagattcgcg aactgctcag
    12781 cgatatcccc gtgtctgtga aggtctattg cgagctcgaa cccgagtcgc gacaggagat
    12841 caaggaggtc tacggcaagc cggtcattgc gaccgagtcc acgacgtcgg agaccgacac
    12901 gacctcaacc atggcgggtg agccgggtgt ggttccgaat accaccgtct ccgtcgccgg
    12961 tggcgcaccc gtcaacaacc atgagaagac aaccagcgat atgacttata agtccgaggc
    13021 cgacaaaacg gtcacccaga ccaagcggcc tcgagatacc atcaaaagca tctcggcgtc
    13081 cgttaccatc cccaagagct atctgctcaa cgttttcaat cttcccaagg agacgtccga
    13141 ccaggagttg gacacggcaa gcaccagcct gcgcgagaaa atcaagaagt cggtgatgac
    13201 cgccctggcg attccgcccg atgccgagga acgcgttacc gtcgattggt ttccggatgc
    13261 cggggccctg gcgttggctc acctcggttc gtccggagcc ggcgagggcg tcatgagcat
    13321 ggtctcaacg cattgggaca aggccggaat ggccgtgctg gccgtcctcg ggctgctgat
    13381 gatgttgatg atggtccgca aagtgggtga agcacctgtg ctgccgggcg aggactcccc
    13441 aaaggccgga aggaaaggaa aactgcccgt ggccgaggtc gaagaagagg tgccggttag
    13501 cgaggcaaag gaaaccgagc cgctcttggt cggaaaagag gtcgacgaag aaaccctacg
    13561 tgcccggcag atcgttgaac aggtgggcga actgatcaag ggagaccccg tgtcctccgc
    13621 aagtatcctc cagcgctgga ttgaggaggc gaacgcgtaa ccgtggcgaa gaagggtaag
    13681 aacaaggaga acgaacaggg cgccgagaag ggattcaccg gcatccgaaa ggttgccgcc
    13741 ttccttctgg ccatcggacc ggaggcggcg ggaagcctct tgcgacgcat ggatcgcgaa
    13801 atcgtcgagg acgtgacccg cgaggtcgct tctctcggag tcgtcccgcc cgaacaacag
    13861 aaggccatcg ttgaagagtt ctataacgtc gctctggcca gacagtatgc caacgagggc
    13921 ggcttgtctt atgcccgcgc cctcctcgag cgggccttgt ccaaggaaga ggccgcccgt
    13981 gtcatgcagc agatcgagca tcaggtgcat cagcagccct tcagcttcct ccagaaggcc
    14041 gagagtgagc acctcctgac cttcatgcag gatgagcatc ctcagaccat cgccctgatc
    14101 cttgcccacc tgcccagcag caagggcagc gagattctgt ccggactccc gcccgccaag
    14161 cagattgagg tcgtcacccg cgtggccaac atggaacaaa ccaaccccga ggtcattcgc
    14221 gaggtcgaaa aaggactcga acagcggttg tcgggcgtca tgacccagca gttccagaag
    14281 gtcggcggcg tcgaggccgt ggccgaaatg ctcaacctgt cggatcgggc gactgaaaaa
    14341 ggcatcctcg aaacccttga ggccgaggac cccgaactcg ttgaacagat ccggcggctc
    14401 atgttcgtgt tcgaggacat cctgctcgtc aatgacaagg ggattcaggc cctgctcaag
    14461 gaaatcgaca acgacgagct ggccctggca ctcaagacgg ccagcgacga gatgaaggag
    14521 aagatcttca gcaatatgtc cgaacgagcg gcccagttga tcaaggaaga catggaatac
    14581 atgggccccg tccgattgag cgacgtcgaa gccgctcagc aacgcatcgt cgacgtcgtc
    14641 agacgtctgg aagatgccgg ggaaatcatc atctccggtc gcggcggcga gtccgacgtg
    14701 gtggtgtgag aggcgcgttg aggaggtcgc ggcatggccc gcagatcaac ggttattaag
    14761 gcggcagagg cacttccctt ccagggcgcg gtactcaagc cgtttcacct cagcgacatg
    14821 atgggtgaag tcaaggccgc cctcatccag gcccaggccg acgccgcccg aatcgtcaga
    14881 caggccaagg cccaggagga cgccatccgc cgggcgggtt acgaggccgg ttatcaggcc
    14941 ggcttcgcga aaggcctcga agaggggcga gaaaaaggcc atgcccaagc ctttgctgaa
    15001 gccaaagccc agttcgccgc gacccacaag agcctcctgt cctcttgcga gaagatcatc
    15061 gcggatatcg aggaacgccg ggccgcctgg caggccgccg cccgccagga tctcgtcgat
    15121 ctggccatgg ccgtcgcgcg ccggatcgtt cgccacgtca gcgaaaacga tcgccgcgtg
    15181 gtggtcgaga acctcgagga ggccattcgt ctcgccggag aacggtccga cgtgaccatc
    15241 aagatcaacc cggccgatgc ccaggcggtt cgtgtgttcg cggagtccct ggccgatcgg
    15301 caggcctcct gcaagctcgt gaaaatcgtt gaagcccccg aaatctcgcc cggcggttgc
    15361 tgggttcaat ggggcagcgg cgcggtcgat gcccgcatcg agacgcagct tgaccgcatc
    15421 gccgcggaac tgggggtccg cggaacaccg tcgattcagc atgccgcgga aaatatgacc
    15481 cgggagggtc cggaaaacgc atgaacgcgt tcgcaaaaga gatcgagatg gtcgactcca
    15541 tggtcactcc gtccctcgtc gggcgggtcg tggacgtcac cggtctgacc atcacggtct
    15601 cgggcctccc cgcgccgatc ggagcgatgt gcacgatcga acggcagaac ggtacccgca
    15661 tcgaggccca ggtcgtcggc ttcagaaacg accacaccat cgtcatgccc ctgcaggaca
    15721 cgctcggcgt ggccagaggc gacaacgtga ccgcctggcc cggcgaggcg aaagtccccc
    15781 tctgcgacga gatgatcgga cgcgtgatgg acggcctggg ccgtgtcatc gacaacggcc
    15841 cgccgctcta ttgcgatacc tgttacccgg tctatcgccc cgctccccgc gccctcgagc
    15901 gccctcggat ctcccatccg ttgtccaccg gcatccgctc aatcgacgcc ctcttgaccg
    15961 ccggtcgggg gcaacgtctt ggactcttct ccggaaccgg cgtcggcaag agcgtattgt
    16021 tgggcatgat cgcccgttac accgatgccg atgtgaccgt cctggcgctc atcggagaac
    16081 gcggccgcga ggtcaatgag ttcctgcaga atgacctcgg gccggaagga cgccgcaaaa
    16141 ccgtactggt actcagcacc tccgacgagt cgcccgtcct gcgcgtacgc gcgggattcg
    16201 tcgccacggc ggttgccgag tacttccgcg atcgcggcaa gaacgtactc ttgctcatgg
    16261 attcaatcac ccgcatcgcc atggctcagc gacagatcgg cctggccgcc ggagaaccgc
    16321 ccgcgaccaa gggatataca cccagcgcgt tcgcgctgct cccgcagttg ctcgagcgcg
    16381 ccggccagac ggagaacggc agcattaccg gcttctatac cgtcctggtc gaggcggatg
    16441 acatcaatga tccggtcggc gacgccgtcc ggggcattct cgacggccat gtctggctct
    16501 cgcgggcatt ggccaacaag tcccattacc ccgccgtcag cgtctccgag agcatcagca
    16561 gagtgatgag ttccatcgtc gatccgcagc atctggccgc ggccagaacc gtcatgaagg
    16621 ccgtcgcgat ctggaacgag atcgaggacc tggtgaatat cggggcatac gccgccggcg
    16681 cgaacgccga atacgacgcc gtgatccaga cccgtcccgc cgtcctggct ttcttgcagc
    16741 agggggtggc ccagcgggtc agtttcgccg aggcacgcca gcagttgctc gccttggctc
    16801 agcagatcga ggaaaccacc cgcagactgt caagggaggg cggccgtgcc cgaacggtca
    16861 cggcggcata acgcaagaga gtagaacatg cccagacgat tcaaattccg gttcgcgacc
    16921 atgctcaaga tccgccgcca gcgcgaggat gagcagaaac gcatcgtggc cgaacggtta
    16981 cgccagatcc gccagacgcg agaccatcgt ctggtcctcc aacgacagat cgaggacgag
    17041 gtcgacgcca tccgccacag ccagaccggc ggggcaatcg atattcaaca ggtcatgagg
    17101 caccgccatt ggctgagcca ccttcataag ggcgtcttgg aagccgacgc ccgcttgcgc
    17161 ttcctggagg cccggctggc acaggaacgc gtggttctgg ccgaggcggt caagcagagg
    17221 aagattctgg aaaaactgaa ggagcgtcaa tggcaacggc acctccgcga gcaaggtgtt
    17281 ctcgaagtga aggaaggcga cgagttggcc gccgttcgat acgtgttcgg ccgcgacgcc
    17341 gatgccgaac tgctgcggac ctgaccgccg ccgtccgcac gtagcctcaa cgaggatcga
    17401 tctgaaccat gttgaagcgt ttctaccatc ttctggcctt gctggcgctg atcaacctct
    17461 tcgccgtggc gggtttcgtc ggctacctgt tcgccagcgg gcgcctcgac gccgagcgag
    17521 ccaggcagat cgccgtcgta ctgagaggcg aattccctgc gtcgcagccg gccgctgccg
    17581 ccggcacgcc gacgccgcgt gatgagagcc cccaagcctc cggcgacgag atcgcccggc
    17641 agcagcagac caaggaactc ctcgcccttc aggagagacg ccgggcacgc gatctgcggg
    17701 accgggatct gctcgacaag cgcatccaac tcgagacccg tcaactgctc gagaagctcg
    17761 agcgcaaaga aaacgaactg aagcagcaga aacaggtctt cgagaaggag ggcgagcagg
    17821 cgggattcga tcgccagctc aacctcttgt ccaagacgga acctaaactc gcgctgcagt
    17881 tgctcaagaa tcagatcaag gaacccgacg cggttcagct tctgatgaaa atggatgaga
    17941 accgcgtcaa aggaattatc aacgcctgta agacggaaga ggataaggcc tggatcggga
    18001 ggatcctgaa ccagattggc aaatatggaa gctcaacggc ggccggtggg gatgattccg
    18061 gcaagcccgc atccgacgga ggatgacatg cccaccgtgg aatgggcgga agtccggact
    18121 gttgcacact cagcgcagac cgtccgcgat aagcccgagc agctcgatga cgtgtttgcg
    18181 gttgtcttcg attcggcccg ccgctcggtc ggctcgcgcg tcgaccggac cgtgcgcgag
    18241 caacagcatc gcgccgccga caaagacaaa gcggcagcta cgcgcgatcc ccaggccgtg
    18301 acctccggcc ggtcgaaaag caagggcgcg gaaggacctc aggaacccaa gaagaaccag
    18361 cagaccgcaa aacccgaatc gccggtgaag ggaacgccga tcgagcccgc tcaggctgag
    18421 tctccgcaac ccgcccctgc cgaggaatcc cctcgggcaa acgaacccct cgttgtcgaa
    18481 cacgcaacgg agccgagcgc cgccgatccg gtcccgcaag tcccgcctga gctgaagccg
    18541 gcgccctccc aaaagagctc ggagcagaca aaagctgcgc aaaacctgct gtggaaacca
    18601 acacccgggg caaccgagtt cccgatggac accgccccgg cagagaaccg ggtagacgac
    18661 tcgccgacga cgcaaacctc gccccagctt gtaatctggg cggttgccga tccggttgcg
    18721 gcagtcatcc cggatatccc ggcgacggct caggcgggcg acgccgcccc tggcgggtgg
    18781 gctcctccgc aggcgactga agtctcggca acccgcgaga caaccggaac ctcttccggc
    18841 gagcaggctc cgcagcctgc ttccggcgcg aacgtccaac cggcacagga actgtcaatc
    18901 tccgtctggc agttccttcg aggcgtcgcc gcccatctgg aacaggcgat cgagacccgg
    18961 gatcgcaggg cgatgaataa acagcctgcc agttgttcag aaccaacggt cgcggcccgg
    19021 ccgaccggcg agacgcctcc caacccgatg acgaaaccgc cgctgctcgg cagcttggaa
    19081 aacggtagct ccacaaacaa gcagttgctc gggcaggacg aaaaccgctc accggaacat
    19141 gatgcggcct cacaagcgtc ttccggcgat gcggccagga acgcggacgg ttccgcccat
    19201 caaaccgaca ccaatgacga gttcaggggt ctgctggaac ttgccggacg tgtacgcgga
    19261 cggatcgtct cgcctgatct tcaacctgcc cccgcctcgt tcgggcgcga tttgggcggc
    19321 cagggcatca gtctgaagga ggcccgggca atgaccgagc tcgccgacgt cgtccgggcg
    19381 aatatcggcg cgaggcattc gtctatggtc ctcaagctcg agcccccgga actcggccgg
    19441 ctcagggtcg acgtccgcac gcacggcgat gaactgacac tgcgactgca ggccgactcc
    19501 ctcttgggac gagatgccct ccagagccgc atcaacgagc tgcggagttc cctcgagcaa
    19561 cacggcttca aactcaacca gatccaggtc gaactgcggg ttccccaggc caatcctggt
    19621 gaagcccatc aggaccggcc gttccagcaa caggaacact cggatggcca gtcttcccat
    19681 gagcccgcat ggcaggggca cgaaggcggc ggaagtcaga ctccttcgga atcagaatgg
    19741 tcgaacgaag ctttcgcgtt ctcccaggag aacgcttcat ccggggagga gcaggatcgg
    19801 gtcgatcgtc tggcggagac gggcgtcgac ctggttgtgt gacaaggcgc gtgcagatcc
    19861 gccccgtcgg aggacggggc ggatccatcc gcctgtcccg gctgcgccgg gacggctgag
    19921 aggacaacaa gatgcagata acagcaacac ggtacacgga cggttcgacc gcgcagacga
    19981 caacccagcg ggatgtgtcc ggcatcagcg gccaggattt catgaagatc ctgattaaac
    20041 agcttcagct tcaggatccc ttcgagccca tgaccaacca ggaaatgatc tcccagatgg
    20101 ccaccatccg ggaactggag atgaacaccc gcctcaccga gcggctcgaa caacttaccg
    20161 atcagcagag agtcgcctct gcttccggac tgatcggcaa gcagatcaag ggcatcgtca
    20221 ccgattcgaa cggaaacgag tatgaactca aaggtcttgt gaccggcatc gagttcacca
    20281 gcagaggcga gatactgctg cggctcgaca acggattgag attgcccatc accagcttga
    20341 ccgaggtcac ggagccgcga tcggatctgg aaacggagga ggattcggag tcggcctcgc
    20401 aggaagaaaa atcagaggct gcggcatgat tcaagggttt cgtccggccg attgtgcgaa
    20461 cggcaacggc cgatgcaagt aatcgacatg acaggctgtc tcggccgcaa gagccgagcc
    20521 ggatcagaag aaaaggagac gggcaatggg attgaccagt gcaatgtata ccggcctttc
    20581 gggcctgaat gccaaccagt atcggatcga caccgtcggc aacaacatcg ccaacgtgaa
    20641 taccaccgcc ttcaagggaa gccgggcgtc attccagaat cagttctcgg tgatgctctc
    20701 cggcggatcc gcccccagca atacctcggg cggcacgaat ccgtcccagg tcggcatggg
    20761 atctttggtc ggctcgatcc agcgcaactt ccagtccggc gccctcgaga ccaccggtgt
    20821 gcccaccgac atggccatcg aaggcaacgg cttcttcatc gtcagaacgc cggaactcag
    20881 gcaggcttac acccgcgatg gcatgttcgc cctcgatgcc tccaacaccc tcgtcacctc
    20941 ggacggatac cccgttcaag gctacggcat tgatgccgct ttcaacatcg atcggtcccg
    21001 cttggtcgac ctcaacatcc ccctggggac gctcaccatc gcccgggcaa cctcatcagc
    21061 cgccttcgac ggcaatctga atgccaacgg gaccataggc acgcaaggca cgattctcta
    21121 ttcgcaggtg ttcaccgacg gtagctgcgg accggccgcc accggcgcaa ctctgctgac
    21181 caacctgtac gaccccaccg tctccacaac cagcgccctc ttcgcggtgg gcgacgtcat
    21241 cacccttcag gatgtccgca agggcggccg acagattgaa acggccagct tcacggttga
    21301 tgccgccagc accctggatg atttcctgac tttcctgaac aacgaactcg gactgaacgc
    21361 cgatccggca gtacccaata cgccgggcat taccgttgcc agcggcttgg gcactccacc
    21421 cgatggaacg atcatcatcg aaggcaacgt cggctacgat aaccgaatcg aaatcgccca
    21481 ggtcggaatc cgcaactcca acgagaccgt caccgccagt ccattccagt tcaccccaca
    21541 gcagcaagcc aacggcgaaa gcgtctatac cagtttcctc gtgtacgact ccctgggcac
    21601 gcccattcag gtcagtgtga ccgctgtcct cgaaagcaag acggatggcg gtaccctctg
    21661 gcggttctac accgaaagca ccgacgacac ggatccgacg ccggtcctcg gagccaccgg
    21721 taccatccag ttcgacaaca acggccgcct cctgaacgtg accaacaata tccttcagat
    21781 cgaccgcgat aacagcggag cctcaaaccc cgtgagcttc gaactggact tcagcaacct
    21841 caccggcttg acgaccgaca tgtcaaccct ggtcatgacc ttccaggacg gctttgaggc
    21901 cggtaccctg accagcttca ataccggtgc cgacggggtc atcaacggtg ttttcaccaa
    21961 cgggctcagt cgccctctcg gccagctcgc ccttgccacc ttcatcaatt gccagggcct
    22021 cgtctcccgg accgacaact tgtattacga agggtcgaat tcgggccagc cgctaatcac
    22081 cgccccgggt aatcttggtg ccggtaccat ccagggagcg actctggagc tcagtaacgt
    22141 cgatttaaca cgcgaattta tcggacttgt cacggcatcg accggttttt cggcgtccgg
    22201 acgggtgatc aacacctcca acgaactgct caatgaattg ttgtcgatag cccgataaga
    22261 aaaggtgcgg cccacgcctg agggcggccc acaggcgctg ggcctcgaaa cgtcccgacg
    22321 ccacagggtg acgcggaaaa ccgaaacgtg atcacactga cccggttgaa cggaacacgc
    22381 ttcgtgatca acgcggagct gatccgcacc gtcgaggagc ggcccgatac gaccatcgtg
    22441 ttgatcaacg gcgagacctt catcgttcgc gaacgcatgg aagaggtcgt cgcccgggcc
    22501 attgagtacg gacgaatgat tcgggtcttc agaccgtagt ggccgccgga agacccgtac
    22561 tcgattcgct tgactcgcgc agcgccccgc cggcaccatg cccgccggct tggctgacct
    22621 cgcctggcag gtgaatcaca tgaaccgcat gaccgatgta agacacaaga tactcggatg
    22681 cacgaatcgt ccgataagtg ccgcgccttg gcacacagga ggtggccatg gttgatcgcg
    22741 cgaccgctgt cgggctcgta ttgacc
//
LOCUS       DAOTPW010000070        22513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2363_length_22513_cov_4.824339, whole genome shotgun sequence.
ACCESSION   DAOTPW010000070 DAOTPW010000000
VERSION     DAOTPW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22513
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2363_length_22513_cov_4.824339"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="P5316_08295"
     CDS             <1..1269
                     /locus_tag="P5316_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR84995.1"
                     /translation="IIFILADDLGYGDVGCYGCGDIQTPQLDSLARAGVRLTNFYVAS
                     PICAPSRAALITGRYPNRIGMSTEKNIESGMDQPGIPSSEITLAELVRPQGYATAALG
                     KWHLGSTHDTQPNAQGFDLFFGHHGSCIDAFSHMYYASLPYYHDLYRNRQEVFEDGMH
                     MTDLITREAVRFIEENKSRPFLIYAAYNAPHYPMVAHGRFHRQYAHLPRARRDYAAMV
                     AGLDESIGIIMGKINELGLTEDTFVFFTSDNGAADPSPRGEGGGSNAPYREYKRSLFD
                     GGIHMPAIISWPKRVRGGQVRDQLAVAMDLFATVAEITGSRLPPDHVIDGRSWMPFLL
                     DPSKPGHDVLYFEWDGQQAVRQGKWKVVRNGLMNMAQGRTTRATGDDVVFLVDLSADP
                     GEKVNVCQEHPDVAQQLLRLHAQWQSMKAK"
     gene            1283..2347
                     /locus_tag="P5316_08300"
     CDS             1283..2347
                     /locus_tag="P5316_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84996.1"
                     /translation="MNRVDLATIAGGLDPSKNAFNGTDANPLILEMYFWAQTGGKEDN
                     TAFFAQLYAEDSRVPVSQTVPDPENPGQYIDVPLRLVHASPCNGSSDIQNITPSVPRK
                     AMAFGFFGGENGLQPKLDCDLTNWMVYQNVIYDGDHWSLLKENFPTPGGQINSSEKWT
                     WLKMTIKSTTFDLDVRSSQYPATQTRRGILRAYTGGFQSLALGTTDYPGPNWTGSDYP
                     CYADNITLTGGVLDLVVPKGACCLPPPSLGCQSNATESDCTALGGIWQGAGSSCDDPN
                     INCCPDPFADADWDHDVDQADFATFQACFTGPGGGVPERCSCFNHVLTGVVQGIDSDD
                     WMAFEACASGPGMPADPACD"
     gene            2432..3943
                     /locus_tag="P5316_08305"
     CDS             2432..3943
                     /locus_tag="P5316_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR84997.1"
                     /translation="MKRSCAIAVVPAVLMSCSSAMAGRCIGVEPRRIIMCDDFDSYCN
                     GGDPWPGYPPSDPPPAACVDGSSVNSVGFLTNWAEMTMLCGFDMKIGSDQKYSMPFGM
                     KYPAGIEDYVDVEEEQRVYDLLRQGAIEMAAVRRGEPAADAVNGTADAPLVLYFVLWL
                     GHEGNRFGQYKDLYVELALGGDRAPTDHVMSEDCSQMSCGGPSKGFPVVCQVANPLEP
                     RPADCPPISTATHAAIAFGLLSKLDMNPCHPELCGTGAHHPQNNHPAFFDGNRWWVVK
                     GGYGGANGDFSLTKAYHRFSMTVTDTQVELRMWAPGATISPYRATYPRQYTGPFDRVR
                     IGVGRGCVLDDMTGTCAAPISYDCINGSHSGNPWVDDVVLYDGVLTRSQGSQGGCCVP
                     LDGCRLMTAGDCAAAGGTYRGDGTNCDDARACCPTPFADIDEDGDVDQTDFSFFQACY
                     TGPGAFALDGICKCFDVTDSAGSGLDSAVDGNDLARFERCGSGPGVPLVPACN"
     gene            complement(4061..5152)
                     /locus_tag="P5316_08310"
     CDS             complement(4061..5152)
                     /locus_tag="P5316_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HRR84998.1"
                     /translation="MAGLIGRPIEDLDTPALLLDWPAAQRNLRKMADFFRGRPAQLRP
                     HFKNHKCSELARRQLKAGSAVGVTCAKLAEAEVLATAGVDDILIANQIVGPAKIDRLI
                     ALARRVPRLRVAVDDLEQTRLLARVAEASGLTVGVLIEIDIGMGRCGLPPGEPAVRMA
                     AELATLKGIRLDGLQAYEGHLVAVADPRERQTRTQQAFEPALHTRQMLVDAGLPPGII
                     SGGSTSTYAITGRIEGIDEIQAGTYVTMDNMYRKLTPEFEVALSVLSTVISRPRSDTN
                     VLDVGLKGLGHEFGSPSPKEWLDGDCQSSLAEEHCIVRNGPDWRVGQKVELVPSHACT
                     TCNLYRQLHVFEQGRIVDVWPIDASGCLT"
     gene            complement(5140..6624)
                     /locus_tag="P5316_08315"
     CDS             complement(5140..6624)
                     /locus_tag="P5316_08315"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRR84999.1"
                     /translation="MNKCWPTQMYIGGRWVSGTGGKTMPVFNPATSEKLTEVAVADAA
                     DVDRAVAAARKAFDEGPWPWMDPLDRARYLWKIAEVLRRRAEEFSMTDTLNIGKPIRD
                     TRGWDTPVSVQLFESYAGLVDKVAGKCFGTLPESFTFLSREPIGVVASIVPWNYPLTN
                     AAIKLAPALACGNCVVLKPSEVAPLTALMLAEIAEEIGLPPGVLNVINGTGAETGSAL
                     VGHPGVDKISFTGRLATGRQIMKLAADGIKGVALELGGKTPNIVFPDARLEDAASAAL
                     MGIYCHLGQVCVAASRLLVHKSIHDEFVDRLLTRAKAFRQGDPTDERNHLGCIATPIH
                     LKTIEQYVEKAKSEKALLLMGGCRPDDPALAKGCYYRPTLFDRVTPDMTIAREEVFGP
                     VLAVMTFGDEDEAVRIANDSDFGLMACLWTQDGGRALRVARRLRVGKVAINGGGAFRS
                     GSPMSGYKQSGIGSDLGHEEAVHEYTNSKVVLYSLATEIPAWPD"
     gene            complement(6768..7883)
                     /locus_tag="P5316_08320"
     CDS             complement(6768..7883)
                     /locus_tag="P5316_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85000.1"
                     /translation="MRHRCLSDRRTVWLSSWVLVAVLPVFFAAVAPAAQVAGEPKADS
                     AVIEEYRQRLSELGPRDIAGHYALAQWCNERGQYELVLERTQYILRLDPRNVDARLLN
                     TAALHRLGQQPVDAGLDGRPPGEITLGLITKQDVQRLRFLELLDFQPDDVPPALRESI
                     SVRFDRGALTAFLDAMADVPEFAGKANRQRFIALKPTQQVQVMRQYGDLAFQPRITIL
                     NDPLVFRRFKPVAALIERGCATRDCHGGSTPAKPFGWRGHSLYPEQSLYTQFLILDRV
                     ALGRDRLIDRDQPADSLILQYGLPTGATRRSHPGQIKPLYPQGTEDPNYKMVLDWIDL
                     LRIPRPINGIRLEGYPEPLPPGSRLSATRPASSRPSR"
     gene            8097..8399
                     /locus_tag="P5316_08325"
     CDS             8097..8399
                     /locus_tag="P5316_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85001.1"
                     /translation="MGWYDERSVALPESLDILIGKEVVLDTEGPMVFIGRLASYNADG
                     FWLEQADVHNCRDGHAQREQYIAESARDGIRVNRERVFVFRRAVVGVSALADVTTD"
     gene            8425..9414
                     /gene="galE"
                     /locus_tag="P5316_08330"
     CDS             8425..9414
                     /gene="galE"
                     /locus_tag="P5316_08330"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRR85002.1"
                     /translation="MRVFVTGGAGYVGSHCVKDLCEHGYEVVVYDDLSRGHRGAVHPK
                     ARLIAGSLGDAAMLESALASQAFDAVMHFAAYAVVGESVDQPLMYYRNNAANTLLLLE
                     LMARIGIRRLVFSSTCATYGTPQRVPIPESAAQSPISPYGRSKLAVEWMLDDSAAAWG
                     LGSCALRYFNACGAAEDGSIGEDHQPETHLIPIVLQTALGQRPHVSVFGSDYPTADGT
                     CIRDYIHVEDLAEAHRLAIEAIRPGEYTAYNVGTGRGHSVREIIDAARRVTGQAIPVS
                     EAPRRPGDPPSLVADATQIRTRLGWQPRWRDIEKVVASAWKWHRLHPRGFGDR"
     gene            9515..11386
                     /gene="lnt"
                     /locus_tag="P5316_08335"
     CDS             9515..11386
                     /gene="lnt"
                     /locus_tag="P5316_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRR85003.1"
                     /translation="MKSSPVGSADRPCDIENDWLVAGLALLTWLLSLPIFEPQSVGLV
                     AGITFVPWTAAVCLARRARRMYLVSYLLGAAFFLTHLRWLYTTTGPGYVAAALYLAAY
                     FPLAAWPVRYLYRDRRISPTFALPIAWVAWELVRSKGLLEFPWFLAGHTQARRLLLIQ
                     IADLGGAYGVSFVVMMVNGWLAERVMRAIQVRRGRKNQETRLAAAVSTVVLLVVVGGT
                     VLYGHYRLSYSGTTTGPRIAVLQGDYLLSATSGLDSGPRKFRQPENDEIDRELRRLGD
                     DGDDEADKRLTYLSLIEKCAAESPDIMVLPETPWWMYLNRELRELPQSVIDEERDSAR
                     RAWLELCWKVANAQHREFQALARKHDCYLVVGALSEERQPPGKYPAEHRYNSAFVYSP
                     DGSSEPMRYDKIHLVVFGEYVPFRYSPHFFWLYRFLNDGWWNPWGRGGREYSLTRGTE
                     HTVFSVKPRSMGDRQVRFGVTICYEDVIPEVFRGFMVGQDGGKGVDFMLNISNDGWFG
                     HGTQQAQHLANCAFRAVENRVSVARAVNTGVSGFINSSGSWYSLVGASDRDPQVGGEG
                     YRVDEIQLDPRVTFYSRYGDLFALTCVGLVVVAVFDCLVVSRLLKQRSTKKRGLSHE"
     gene            11379..12440
                     /locus_tag="P5316_08340"
     CDS             11379..12440
                     /locus_tag="P5316_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR85004.1"
                     /translation="MSECRCAAQFPAREMTVWVVLLVLTTLATGCSFEPTPDFITAVR
                     QEARTCIERGMVYPFDPAVRAQAMEAASKVLGDSGRLRIREGLKDQHPGVRFAAAMAL
                     GKLRDKAAVPAMEALTNDPDVSVRLAAYFALEQCGLPTTERRTAWRDHLRKHENPEVR
                     RNAVLALGQLGDRSTVPLLRAAASGDSDEGVRTQALEGLAMLGDKDAISRFIFEAFGG
                     VGYRQPFAVMTLGQVPDDRVVPILRSRLASSPYLEAKLAAARGLGAKGYADGLGLAMR
                     SLDWNKPDRSLAEDRPENQIMRVRSMAAMALGEIGDPRALGMLKKTMENPEDPRVQLA
                     AATAILMIVDREALQPSVR"
     gene            12543..13082
                     /gene="mreD"
                     /locus_tag="P5316_08345"
     CDS             12543..13082
                     /gene="mreD"
                     /locus_tag="P5316_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRR85005.1"
                     /translation="MKWMPFAVLAIVVIVCQTTFVQALLAIQAIRPQWTIILAVHYAL
                     WAPSPDAAIAAWILGLIVDLQTASPHPIGLYAFSYGLAAWVVLRSRQVLFRDHPVTHA
                     VVTLAFAFAIQVIAGLYFWWKHRIPIDMEGFWRPAFLFAGYTAIWAPCLHWLLIKLQR
                     WTGLGPMRRRMTFRPENPA"
     gene            13281..15434
                     /locus_tag="P5316_08350"
     CDS             13281..15434
                     /locus_tag="P5316_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRR85006.1"
                     /translation="MFERRLRLIMVLMVMPVIAVTARLVQLQILEGEEYASKAEALLI
                     KPMRLYPCLRGEIVDSEGVRLAYDAESWDICVHYGILSEAEDHLRAMARERLGRRGIP
                     RTADNVAEEMHKLGIEINESWHAIARLTGRQVEELDAVRERRLRQVQRIKEVVTRRRK
                     IDTVVDEERTVHPIVQGLNHQMCVEAKVQLAEYPWVEVLVSHTRRYEGGDAMGHLYGM
                     LNEVGPDDLRENSNNADILSRYLLGDLIGRTGLERLGEQWLRGRRGCEQEDLRGVPTT
                     QPVAPVDGNTMRLSLNLRLQQYCYHRLQAAVREHPPSTGGAAVILDVPTRRVLALVSY
                     PAFDPNLPDTERAKLAAQDPFGQPHVFRAVHQPYPPGSTIKAMVLPSALTEGKVGPGT
                     TYDCVGHLFPGLPNKWQCDARRAHGTVDPVLAVQKSCNVFFYHVGENMGLANLVKWMD
                     RFGFGRVTGIGLIEDATGTLPQLRRDMGAGPARLAAIGQGEVSATPLQLANMMATVAS
                     GVWKPVTIWPDNPDQSLQGYALPIPAANWRLVREGLYKVVNEQGGTAYGPLRAKLAGE
                     EEFVLLGKTGSAEAPALERLYRCRFPDGTEKTIKARTFRELRNRYPEGQKPEYISQED
                     AQVYPTHGWFVGYLTTRGHYLEPAIDGELNVAIAVIVEYAGHGGAVAAPVARDMLQAM
                     ITLHRGGTIETAPADDSTVRSAAAVTDAAGEGERP"
     gene            15431..16705
                     /locus_tag="P5316_08355"
     CDS             15431..16705
                     /locus_tag="P5316_08355"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRR85007.1"
                     /translation="MSSLFSTRGGWIILLPVLPLLVAGVLTIQATNASDQPGRLADET
                     VKQIAYGAAGIGCMILIVTIGYLRIGRFSYILFGISILVLACLVIDRWFDLPFVPVKR
                     NTRRWLQVGGLQVQPSEMMKVAYVLGLAWYLRYRSNYRTLLGLVPPFMLALVPMGLIK
                     LQPDLGTLLLFLPVLFVVLYAAGAKGKHLAAVIVMGLLCSPLFWTKIEPYQQLRITSV
                     MLQNRAVRDYLSKPMPSRPDLPTRWEYFRPKEFSQSRWMLELANWENVTGYQLTRSKI
                     ALGSGGLFGQGSGRGVFVQYDFLPERHNDFIFAMIGHQWGIIGGVLTLLCYGLIVVFG
                     YEIAVLTREPFGRLVAVGLTTMLAFQAITNICMAIGLGPVTGVPLPFVSAGGSSLVIN
                     FMALGLIISVARDRPLLFTNRPFEFETEEEED"
     gene            16900..18054
                     /locus_tag="P5316_08360"
     CDS             16900..18054
                     /locus_tag="P5316_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR85008.1"
                     /translation="MADDQLTPETLNAAQPKNMAVAAKMREFLSQGRWAPGDQIPTAR
                     QLAQMFGVSLSPVLQALQILEKEGLLLRRARVGTFVAERRAKKDLLAFITATCDLEPY
                     IHSLWGFEEEASRRGYHILFCNANRVPERLAQMANACIEQGAAGIAFTPPEGSFYMDW
                     NRAFYKGVAEHGTPLVAITSQPFPEEDPAIISAAIPDGVWQGRRMAAHLIDQGHTHLA
                     FLYGRWSCTIEDRKQGVIAEAKERGLPPVDVRSVSPEELLRTGEVYDVTAMLGDWLGQ
                     DPPITAVVCCSDYAARIVYEHASTLGMSIPEDIAVVGFDDSSFAPLLGDGGLTTIRVS
                     YIEMGRSAAELLIAQINDPGRPPRILMSRGELVVRKSCRFLPSQVESDRR"
     gene            complement(18195..19514)
                     /locus_tag="P5316_08365"
     CDS             complement(18195..19514)
                     /locus_tag="P5316_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRR85009.1"
                     /translation="MLESRLLSKINRKQARVGILGMGYVGLPLARSFCGVGMPTVGFD
                     IDPRKIKMLNAGRSYIKHIPDQAVSAMRSSKRFEATTNPKVLRGCDALIICVPTPLSK
                     TRDPDMTYVIKTTETIATQLRRGHLVSLESTTYPGTSKEVLKPILEKTGLKAGKDFYL
                     AFSPEREDPGRKDFTTETITKLVGGHDPASLRVAVALYRQAIKNIVPVSSCEVAEAAK
                     ILENVYRCVNIAMVNELKMLFHRMGIDVWEVIEAAKTKPFGFQAFYPGPGLGGHCIPI
                     DPFYLTWKARQFGVTTRFIELAGEINTAMPEYVVEHTARALNERAKALKGSKILVLGL
                     AYKKNIDDIRESPSIELIEIFKKRGCKVDYNDPYIPTTHKQREHDLKMKSVPLTAANI
                     RKYDCVVISTDHDCYDYGMIVRNAKVVVDTRNACARVKGPKKNVYKA"
     gene            19757..20866
                     /locus_tag="P5316_08370"
     CDS             19757..20866
                     /locus_tag="P5316_08370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR85010.1"
                     /translation="MEIALSRPDITQAERDAVLEVLQTPNLSLGPKLPEFERVFAEYV
                     GSRYAVAVNSGTSALHLCIRAIGLKPGDEVITTPFSFIASSNCILFEGGKPVFVDIHP
                     ETWNIDPDLIEAAITPRTRAILPVDVFGHIADMPRIRQIADKYGLRVIEDSCEALGAA
                     LNGKKAGTFGDAGTFGFYPNKQMTTGEGGMIVTDDGQIRDLAVSMRSQGRGSGGGWLA
                     HERLGYNYRLSDINCALGIAQMKRVDEILSHRLNSANYYLVRLSDEKRISMQKALPGS
                     QVSWFVMVVRLNDDYSAEDRDQILVRLRQQGIGCSNYFPAIHLQPFYAREFGYKRGQF
                     PICEALCDRTVALPFHGLLREEEVDIACEAFRRLL"
     gene            21050..22315
                     /gene="icd"
                     /locus_tag="P5316_08375"
     CDS             21050..22315
                     /gene="icd"
                     /locus_tag="P5316_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRR85011.1"
                     /translation="MALPTPLAGDPIRKSSSKLEVPDHPVIPFIAGDGTGPDIWRASQ
                     LVFDAAVAKAYGGKRKIAWHELLAGEEAYKRTQNWLPDETVEAFRTYLVGIKGPLTTP
                     VGGGIRSLNVALRQLLDLYVCLRPVRYFKGVPSPVRNPELVNMVIFRENTEDIYAGIE
                     FQAGTPDAEKFAKWLQEVFPDRFRKIRFPATAGYGIKPVSAEGTKRLVKAAIDYAIAD
                     RRKSVTLVHKGNIMKFTEGSFRDWGYELAKEAYGATPLDGGPWCRLPADKGSVLIKDV
                     IADAFLQQILTRPAEYDVIATPNLNGDYISDALAAQVGGIGIAPGGNINYDSGHAIFE
                     ATHGTAPKYAGKDQVNPGSLILSGEMMLRYMGWTEAADLILKGMDGAISARTVTYDFH
                     RLMVNEGVDAKLLKCSEFGQAVVEHMGEV"
BASE COUNT         4280 a         6619 c         7133 g         4481 t
ORIGIN      
        1 atcatcttta ttctcgccga cgacctcggc tacggcgacg tcggctgtta cggttgcggt
       61 gacattcaga cgccccaatt agactctctg gcccgcgcgg gcgtccggtt gaccaacttc
      121 tacgtggcct cgccgatctg tgccccgtcg cgggcggcgc tgatcaccgg ccgatatccc
      181 aaccggatcg gcatgtccac cgagaagaac atcgagtcgg gaatggatca accaggaatt
      241 ccctcctcgg agataacgct tgccgaactc gttcgccccc agggctatgc gacggcggct
      301 ttgggcaaat ggcacctcgg ctccacgcat gacacccagc ccaatgccca gggcttcgac
      361 ctgtttttcg gccatcacgg cagttgtatc gacgcgttct cgcacatgta ctacgcgagc
      421 ctgccgtatt accacgacct gtaccgcaat cggcaggaag tgttcgagga cggcatgcac
      481 atgaccgacc tgatcacgcg cgaggccgtg cggttcatcg aggaaaacaa gagccgcccg
      541 tttttgatct acgcggccta taatgctccg cactatccca tggtcgctca cggacgcttt
      601 caccggcaat atgcacatct gccgcgagcc cggcgcgact acgcggcgat ggtcgcaggt
      661 ctcgacgagt cgatcggcat catcatgggg aagatcaacg aactggggct gaccgaggac
      721 acgttcgttt tcttcaccag cgacaacggg gcggcggatc cctctccccg cggcgaaggg
      781 ggcggcagta atgccccgta tcgtgaatac aagcggagtc tcttcgatgg cggcatccac
      841 atgccggcga tcatcagttg gcccaagcgg gttcgcggcg ggcaggtccg cgaccagctg
      901 gccgttgcga tggatctgtt cgccaccgtc gcggagatca ccggctcgcg gttgccgcct
      961 gaccacgtga tcgatggtcg cagttggatg ccgtttctgc ttgacccttc caagccaggc
     1021 cacgacgtcc tgtacttcga gtgggacggg cagcaggctg ttcggcaggg caaatggaaa
     1081 gtggtccgca acggcctgat gaacatggcc caagggcgaa cgacccgcgc cacgggggat
     1141 gatgtcgtct tcctggtcga cctgtcagcc gatccgggag aaaaagtcaa cgtatgtcag
     1201 gagcatcctg atgtggctca gcaactcctg cgcctccatg cacaatggca atccatgaag
     1261 gcgaagtgag tatgcggctc agatgaacag agtggacctg gcgaccatcg ccggcggcct
     1321 ggatccctcc aagaacgcct tcaacgggac cgacgccaac ccgctgattc ttgagatgta
     1381 tttctgggca cagacgggcg gcaaggagga caacacggcg tttttcgccc agttgtatgc
     1441 cgaggacagc agggttccgg tgagtcagac ggttccggac cctgagaatc ccggccagta
     1501 cattgacgtg ccgctgaggc tggtccacgc ctcgccatgt aacggctctt cggacatcca
     1561 gaacatcacg ccaagcgttc cccgcaaggc gatggccttc ggatttttcg gcggggagaa
     1621 cgggctccaa cccaagcttg actgcgatct aaccaactgg atggtttacc agaatgtcat
     1681 ctacgacggc gatcactggt ccttgctcaa ggagaatttc ccgacgccgg gtggtcagat
     1741 caattcatcg gagaagtgga catggttgaa gatgaccatc aagtccacca ctttcgatct
     1801 cgacgttcga agctcgcagt atccggcaac ccagacgcgt cgcggcattc ttcgcgctta
     1861 tacgggcggc ttccagagtc tggcgctcgg aaccaccgac tatccgggtc ccaactggac
     1921 cgggagcgat tatccatgct atgccgacaa tatcacgctt accggcggcg tgcttgacct
     1981 ggtcgtgccg aaaggtgcct gttgtctgcc gccgcccagc ctcggttgtc agtcgaacgc
     2041 caccgagagc gattgcacgg ccctgggagg catctggcag ggtgccggct cttcgtgcga
     2101 cgatccgaac atcaactgct gcccggatcc gttcgccgac gccgattggg atcatgacgt
     2161 ggatcaagcc gatttcgcga ccttccaagc ctgcttcact ggcccgggcg gcggagtgcc
     2221 cgagcgctgc tcgtgcttca accacgtgct cacgggcgtc gttcagggta ttgattcgga
     2281 cgattggatg gcctttgagg cctgcgccag cggtcctggc atgccggcgg atccagcttg
     2341 cgattgaggc tgcagccggt tttcgttcga ttcctcatac aacgtcgccc ggcgtgtggc
     2401 gaagccgatg gcccagacag gagggccgaa catgaaacga tcgtgtgcta tagcggtcgt
     2461 gccggcggtg ctgatgtcct gtagcagtgc catggccggc aggtgtattg gtgtcgaacc
     2521 ccgccgcatc atcatgtgcg atgacttcga cagctactgc aacggcggcg acccttggcc
     2581 gggctatccg cccagcgacc cgcctccggc cgcctgtgtg gacgggagca gcgtcaattc
     2641 ggtcggcttc ctgaccaatt gggcggaaat gaccatgctc tgcgggttcg acatgaagat
     2701 cgggagcgac cagaagtaca gcatgccgtt cggcatgaag taccccgccg gcatcgagga
     2761 ctatgtcgac gttgaggagg aacagcgggt ctatgacctg ctgaggcaag gggccatcga
     2821 gatggccgcc gttcgccgcg gtgagcccgc agcggacgcc gtcaacggca cggcagatgc
     2881 cccgcttgtg ctttacttcg tgctctggct cgggcacgag ggcaacaggt tcggccaata
     2941 caaggatctt tacgtcgagc tggctcttgg cggtgatcga gctcccaccg accatgtgat
     3001 gagcgaagac tgctcccaga tgtcctgtgg cgggccgtcc aaaggctttc ccgttgtttg
     3061 ccaggtagcc aacccgcttg aacctcggcc ggccgactgt ccgcccatat ccacggccac
     3121 tcacgccgcc atagcgtttg gcctcctttc gaagctggac atgaatccgt gtcaccctga
     3181 actgtgtggc accggcgccc atcacccgca gaacaaccac ccggcattct ttgacgggaa
     3241 tcgctggtgg gtcgtcaagg gcggttatgg cggtgccaac ggggatttca gccttactaa
     3301 ggcctatcac cgcttcagca tgaccgttac tgatacgcag gtggaactca gaatgtgggc
     3361 tccgggagca acgatcagcc cgtacagggc cacctacccc cgccagtaca ctggcccttt
     3421 cgacagggtt cgcattggcg tcggacgagg atgtgtgctt gatgacatga caggcacatg
     3481 cgccgcgccc atcagctacg actgcatcaa cggttcccac agcggcaatc catgggtgga
     3541 cgacgtggtt ctgtatgacg gagtgctgac tcggtcgcaa gggtcgcagg gaggatgctg
     3601 cgtgccgctg gacgggtgca gactcatgac ggccggcgac tgcgcggccg cgggcggaac
     3661 ctacagagga gacggcacga actgcgatga cgcgagagcc tgctgcccga cgccgtttgc
     3721 ggacatcgat gaggacggcg acgtggatca gaccgatttc tcattctttc aggcctgtta
     3781 caccggtccc ggggcctttg ctctcgacgg tatctgcaag tgctttgacg tcaccgacag
     3841 cgcgggtagc ggcttggaca gcgccgtgga cggcaacgac ctggccaggt ttgaacgatg
     3901 cggcagcggt cctggggtcc cgcttgttcc ggcctgcaat tagccgcagg agcgcgcagg
     3961 taagccggca gaaagtgata tctgtcgcat catcgcggcg gatagggtca gaaagccatc
     4021 tcctgtgttt ctgaatctgg cggttgcgat cattgatacc tcatgtcagg cagccgcttg
     4081 catcgatagg ccacacatcg acaattcgac cctgctcgaa gacgtggagt tgccggtaga
     4141 gattgcaggt ggtacaggcg tggctgggta cgagttcgac cttctggccg acccgccaat
     4201 cggggccgtt gcggacgatg cagtgctcct cggcaagcga tgactggcag tcgccgtcga
     4261 gccactcttt gggcgatggg gagccgaatt cgtgtcccag gcccttgaga ccgacgtcca
     4321 gaacgttcgt atccgatcgg ggacggctga tcaccgtgga caacaccgag agggccacct
     4381 cgaactccgg cgtcagtttg cggtacatgt tatccatggt gacgtaggtg ccggcctgga
     4441 tctcgtcgat accttcgatg cgtccggtga tggcgtaggt cgaggtggag ccgccgctga
     4501 tgatgcccgg cggaagtccc gcgtccacga gcatttgccg cgtgtgcaaa gccggctcga
     4561 aagcctgttg cgtcctggtc tgccgttccc gaggatcagc aacggccacc agatgcccct
     4621 cgtaggcctg caggccgtca agacggatac ctttcaaggt cgccagctcg gcggccatac
     4681 ggacggccgg ctcccctggc ggcaagccgc agcgccccat gccgatgtcg atctcgatca
     4741 gcactccgac ggtcaggcca gaagcttcgg ccacccgggc caggagacgc gtttgctcca
     4801 gatcatccac ggcgacgcga agcctgggca ccctgcgggc aagggcgata agacgatcga
     4861 tcttggccgg gccgacgatc tggttcgcga tcaggatgtc atcaacgccg gccgtggcca
     4921 gcacctcggc ctccgccaac ttggcgcagg taactcccac cgctgaaccg gccttgagct
     4981 gacgtcgggc cagctcgctg cacttatggt tcttgaagtg tggtcggagt tgagcgggac
     5041 ggccgcgaaa gaagtcggcc attttccgca ggttccgctg cgccgcaggc caatccagca
     5101 gcagcgcagg agtgtcaagg tcttcgatcg gtctgccgat caatccggcc atgcgggtat
     5161 ctccgtggca agagaataga ggacaacctt gctgttggtg tattcgtgga cggcttcctc
     5221 gtggcccaag tctgacccga ttccgctctg tttgtatccg gacatcggcg aaccggagcg
     5281 gaacgccccg ccgccgttga tggcgacctt gccgacgcgc aaccgccgtg cgactcgcag
     5341 ggcccgaccg ccgtcctgag tccacaggca ggccatcaag ccgaaatcgc tgtcgttagc
     5401 gatacggacc gcctcatcct catcgccgaa agtcatcacg gccagaaccg gtccaaaaac
     5461 ctcctcccgt gcgatggtca tgtcgggggt cacgcggtcg aacagtgtcg gccggtaata
     5521 gcagcccttg gccaaggcgg ggtcgtccgg ccggcagccg cccataagca gcaaggcttt
     5581 ttcgctcttg gccttctcga cgtactgctc aatggtcttc agatggattg gcgtagcgat
     5641 gcagccaagg tgatttcgct catcggtcgg gtctccctgg cggaacgcct tggctctggt
     5701 caacaggcga tccacgaact cgtcgtggat gctcttgtga accagcaacc gcgacgcggc
     5761 cacgcagacc tggccgaggt gacagtagat gcccatgagg gcggcgctgg ccgcgtcttc
     5821 gagtcgggcg tcagggaaga cgatgtttgg cgttttgccg ccgagctcga gggcaacccc
     5881 tttgatgccg tcggctgcga gcttcataat ctgccgaccg gtggctagac ggccggtgaa
     5941 gctgattttg tctacaccgg gatggccgac gagggccgag ccggtttccg cacccgtccc
     6001 gttgatcacg ttcagcacgc ccggcggcag gccgatttcc tcggcgattt cggcaagcat
     6061 aagagccgtc agcggagcta cttccgacgg cttgagcacc acgcagttgc cgcaggccag
     6121 ggcgggagcg agcttgatcg ccgcgttggt caacgggtag ttccagggaa cgatcgaagc
     6181 cacgactccg atcggctcgc gggacagaaa agtgaagctc tcggggaggg tgccgaagca
     6241 cttgcctgcc accttgtcga ccaagccggc atagctctcg aacaactgga cggacaccgg
     6301 cgtgtcccag ccgcgcgtat cgcggattgg cttaccgatg ttgagggtgt cggtcatcga
     6361 gaactcttcc gcccggcgtc ggagcacctc ggcgatcttc cacaggtagc gggctcggtc
     6421 caggggatcc atccagggcc aaggcccttc gtcaaatgct ttgcgtgccg cggctaccgc
     6481 ccgatcaaca tccgcggcgt cggccaccgc cacttccgtc aacttctcgc ttgtggcagg
     6541 attgaagacc ggcatggtct tgccgcccgt gcctgagacc catctcccgc caatgtacat
     6601 ctgtgttggc caacatttgt tcatgacgcg ctcccgcagg cagaaggaaa agaccgcagt
     6661 accgccgaga ctccctcggc gggagtattg taaccacaat gcgtccagta gtccgtcaag
     6721 tttgtctgat gccacacgac aagccgagcg cggaggcgga agccggttca gcgcgaaggc
     6781 cggctggagg ccggcctcgt cgcggacaac cgggagcccg gaggcagggg ttcgggatac
     6841 ccttcgagcc tgatgccgtt gattgggcga ggaatgcgca gcagatcgat ccaatcaaga
     6901 accatcttgt agttcggatc ctctgtgccc tgcggataaa gcggcttgat ctggcccggg
     6961 tgacttcgtc gcgtggctcc ggtaggcaaa ccgtactgga gaatgaggct gtcagcgggt
     7021 tggtcacgat cgatcagacg gtctcgtccc agcgcgaccc gatccagaat gagaaactgt
     7081 gtgtaaaggc tctgttcggg gtagagagaa tgaccgcgcc agccaaacgg tttggccggc
     7141 gtgctgcctc cgtggcagtc gcgggtcgcg caaccgcgct cgatgagagc ggcaaccggc
     7201 ttgaacctgc ggaacaccag aggatcgtta agaatcgtga tcctcggctg gaaagccaga
     7261 tcgccgtatt gccgcataac ctgtacctgc tgcgtcggct tcaaggcgat gaaccgctgt
     7321 cgattggctt tgccggcgaa ttcgggaacg tcggccatcg cgtccagaaa agcggtgagg
     7381 gcgccccggt caaagcggac cgagatcgat tcccgcaggg ccgggggcac gtcatccggc
     7441 tggaaatcga gcaactcgag gaaccgcagg cgctgaacat cctgcttggt aatcaggccg
     7501 agcgtgatct cgcccggggg ccggccgtcg aggccagcat cgaccggttg ctgacccagt
     7561 cggtgcaggg cggccgtatt caacaatcgg gcatcgacgt ttctcgggtc caatctcagg
     7621 atgtactgag tgcgttcgag aaccaactca tactgccccc gctcgttgca ccactgggcc
     7681 agggcatagt gcccggcaat atcccgcggc cccagctcgc tcagtcgttg acgatactcc
     7741 tcgatcacgg cgctatcggc cttcggctcg ccggcgacct gagcagcggg cgccacggcg
     7801 gcaaagaaaa ccggcagaac cgccaccaga acccatgagg aaagccatac ggtccgtcga
     7861 tcgctcagac accgatgacg catcaacaag gccctccaac agatctcgtc ttcaactgct
     7921 tttcaaggcc cggtcagacg atctggattg taccggaatg cacggctgcg gggcaaaggg
     7981 gcgtcggctg gttgggtcca tccggtcgca agcgcgacat cgcgggatcg caaccctttt
     8041 acttgaccgt ttcttgtcgg caaacgtact atacgctgac gagaaagcgg tgaccgatgg
     8101 gatggtacga cgaaaggagc gtcgcgttgc cggagtcgct ggacatcctg attggaaagg
     8161 aagtggtgct ggataccgag ggaccgatgg tcttcatcgg ccgtctggct tcgtacaacg
     8221 ccgatggttt ctggctcgag caggccgacg tccacaattg cagggacggg cacgcccagc
     8281 gcgaacagta catcgccgag tcggcaagag atgggatacg cgtgaaccgg gagagggttt
     8341 ttgtcttccg acgcgccgtg gtcggcgttt cggccctcgc cgatgtcacg acggattgat
     8401 gaacaaccgg tgggattctg caacatgcgc gtatttgtga cgggcggcgc cggctacgtc
     8461 ggcagccatt gcgtgaagga tctgtgcgaa cacggctatg aggtagtggt gtacgacgat
     8521 ctttctcgcg gacatcgtgg ggcggtgcat cccaaggccc ggttgatcgc cggcagcctg
     8581 ggcgacgcgg ccatgctcga gtcggcgctg gcgtcgcaag cgtttgacgc ggtgatgcac
     8641 ttcgcggctt atgcggtcgt cggggaatcg gtcgatcagc cgctcatgta ctatcgcaac
     8701 aacgcggcca acacgctctt gctgctggag ctgatggccc gaatcggcat tcgcagactg
     8761 gtcttcagct ccacttgcgc cacctacggc acaccccaaa gggtgcctat tcccgagagc
     8821 gcggcccagt cacccatcag tccctatggc cgatcgaaac tggcggttga atggatgctg
     8881 gacgactcgg ctgccgcttg gggcctgggt tcctgcgcct tgcggtactt caacgcgtgc
     8941 ggtgcggcgg aggacggatc gatcggtgaa gatcatcagc cggagacgca cctgatcccg
     9001 atcgttctgc aaaccgccct cggtcaaagg cctcacgtga gcgttttcgg ctctgactac
     9061 ccgacggcgg atggaacgtg catacgcgac tatatccatg tggaagacct ggcggaagcc
     9121 catcgcttgg cgatcgaggc aatcaggccg ggcgagtaca cagcctacaa cgtcggcacc
     9181 gggcggggac acagcgtccg agagatcatc gacgccgccc gccgcgtgac ggggcaagcc
     9241 atccccgtga gcgaggctcc tcgacgtcca ggtgatccgc cgagcctcgt tgccgacgcg
     9301 acgcagattc gaacacgact gggctggcag ccccgctgga gggacatcga gaaagttgtc
     9361 gcctcggcat ggaaatggca tcggttgcat ccgagaggtt tcggcgaccg atgagggatg
     9421 ctgggcgagc ttggcgcggc cgcctgacgg acgacaacag aatgtcacgg atggacagct
     9481 caaggtgaag cgaggatcca aagacaaacc ccgagtgaaa tcttccccgg ttggctctgc
     9541 tgaccgaccc tgtgacattg aaaacgactg gctggtcgcc gggcttgcct tgctgacgtg
     9601 gttgttgagc ctgccgatct ttgaacccca atcggtcggg cttgtggcgg gaatcacctt
     9661 tgttccctgg accgctgccg tatgcctggc gcgccgagcg cgacggatgt atcttgtcag
     9721 ctatctcctt ggtgcggcct tcttcctgac gcacttgcgg tggctctaca cgaccaccgg
     9781 tcccgggtac gtcgcagcgg ctctctacct ggcggcgtat ttcccgttgg cggcctggcc
     9841 agtgcggtat ttgtatcgtg acaggcggat cagtccgacg ttcgctctcc cgattgcctg
     9901 ggtcgcatgg gaactggtcc ggtcaaaggg gcttcttgag tttccttggt ttctcgcggg
     9961 ccacacccag gcaaggcggc ttctgctgat ccagattgcc gatcttggcg gcgcgtacgg
    10021 cgtgtcgttc gttgtgatga tggtcaacgg ctggctggct gagcgcgtga tgcgggcgat
    10081 ccaggtccgg cggggccgga agaatcagga gacccgcctc gccgcggcag tgtcgaccgt
    10141 ggtcctgctt gtcgtggtgg gcggcaccgt actatacggg cactaccgct tgagttacag
    10201 tggcacgaca acaggcccgc gcatcgcggt tcttcagggc gattacctgc tgtcggccac
    10261 ctcgggactc gacagcggac ctcgaaagtt caggcagccg gaaaacgatg agatagaccg
    10321 cgagcttcga aggcttgggg atgatgggga tgatgaggcc gacaagaggt tgacctacct
    10381 gagtctcatc gagaaatgcg ccgcagaatc gccggatatc atggtgctgc ccgagacgcc
    10441 gtggtggatg tatctcaatc gcgaactccg ggagcttccc caatcggtca tcgatgagga
    10501 gcgggacagt gccaggagag cgtggcttga gttgtgctgg aaggtggcca atgctcagca
    10561 tcgagaattc caggcccttg cgcgcaaaca cgactgttac ctggtggttg gggccctgtc
    10621 cgaggaaagg cagcctccgg gcaagtaccc ggccgagcac cgctacaact cggcgttcgt
    10681 ctactctccc gacggcagct cagagcccat gcgatacgac aaaatccatc tggtcgtttt
    10741 cggcgaatac gtgcctttcc gctacagccc ccacttcttc tggctgtacc ggttcctgaa
    10801 cgacggatgg tggaacccct ggggtcgcgg agggcgcgag tattcgctca cacggggcac
    10861 cgagcacacg gtgttctccg tgaaaccccg ttcaatgggc gaccggcagg ttcgtttcgg
    10921 cgtgacgatc tgctatgagg acgtgattcc agaggtgttc cgcggtttca tggtcggtca
    10981 ggacgggggc aaaggcgtcg acttcatgct caacatcagc aacgacggct ggttcgggca
    11041 cggaacgcaa caggcccagc atctggccaa ttgcgccttc cgggccgtcg agaaccgagt
    11101 cagtgtggct cgtgcagtca acaccggcgt cagcggattc atcaactcga gcggatcgtg
    11161 gtatagtctt gtcggcgcat ccgatcggga cccgcaagtg ggaggcgagg gatatcgtgt
    11221 cgatgagatt cagcttgatc ctcgtgtgac cttctacagc cgttacgggg atctttttgc
    11281 cctgacctgt gtggggcttg tagtagtggc cgttttcgac tgtctggtgg tatcacggct
    11341 gctcaaacag cgttctacga agaaacgggg cctcagccat gagtgaatgt cgatgcgctg
    11401 ctcagtttcc ggcgagagag atgacggttt gggtcgtcct tctggtcctg accaccctgg
    11461 ccaccggttg tagtttcgag ccgacgccgg atttcatcac ggcagttcgt caggaggccc
    11521 ggacctgcat cgaacgaggc atggtctatc cgtttgatcc ggccgtgagg gcacaggcga
    11581 tggaagctgc ctcgaaggtt ctgggggatt ccggccgact ccgcatccga gagggactca
    11641 aagatcaaca tccgggtgtg cggttcgccg cagccatggc cttgggcaag ctgagagaca
    11701 aggcggcggt gccagccatg gaggcgctca ccaatgaccc ggatgtgagc gtgcggcttg
    11761 ccgcctactt cgccctggag caatgcgggt tgccaaccac ggagcggcgc acggcatggc
    11821 gggatcacct gaggaagcat gaaaaccccg aagtgagacg aaatgccgtc ttggctcttg
    11881 gccagttggg cgacagatct accgttccgt tgcttcgcgc tgcagccagc ggcgattcgg
    11941 atgaaggcgt acggacacag gccctcgaag gattggcgat gctgggtgac aaggatgcca
    12001 taagccggtt tatttttgag gcgttcgggg gagtcggata caggcagccc tttgcggtca
    12061 tgactttagg tcaggttccc gacgatcggg ttgtgcccat actacgttcc aggttggcca
    12121 gctctccata cttggaggct aagttggctg cagcaagagg gcttggggcc aagggatatg
    12181 cggatgggct gggactagcg atgagatccc tggattggaa caagcccgat cgaagcctgg
    12241 cggaagaccg tccggagaac cagattatgc gtgttcggtc gatggcggcc atggctttgg
    12301 gggagatcgg tgatcctaga gctttgggca tgctgaagaa aaccatggag aatccggagg
    12361 atccgcgcgt tcaactggcg gccgccacgg ccattctcat gattgtggat cgggaggcac
    12421 tacagccatc ggtccggtaa gtcgtcgatg tgagttgtcg ataatattgg cattcgccgc
    12481 cgccgcgagc ggtttgtcgt ccaaggcagc aagcggaatg cacgagggtc gttgtgaatt
    12541 ccatgaaatg gatgccattt gcggtcctgg cgatcgtggt cattgtctgc cagaccacgt
    12601 tcgtccaggc gctcttggcc atccaagcca tccggcccca gtggacgatc atcctggcag
    12661 ttcactatgc cctgtgggcc ccctcgcctg atgcagccat agcggcctgg attctggggt
    12721 tgatcgtgga tctacagacc gcctcacctc atccgattgg gctgtatgcc ttttcctacg
    12781 ggttggcggc ctgggtcgtt cttcggagcc gacaggtgtt gtttcgagac cacccggtga
    12841 cccatgcggt tgtgactctg gcatttgcct ttgcaatcca ggtcatcgca ggtctttact
    12901 tctggtggaa gcaccgcatt cccatcgaca tggaagggtt ctggagaccg gcttttttgt
    12961 ttgccggata cacagccatc tgggcgcctt gtcttcactg gctgctgatt aagcttcaac
    13021 gctggacagg gctggggccg atgagacgcc ggatgacttt caggccggag aaccctgcct
    13081 agggccggtt gacgatcggg gatggcggcc gaggcgtcag ggcccgcccg acaaccggct
    13141 cggagggcgg ctgttatggg cgaacgtgga ctgcttcgga ctcgtcgggg gcgcgaggca
    13201 ttcttgccgt ccggcccggt gctgaggggt ttcggggaat ccggagcggg agctgacccg
    13261 cgtcgtttcg gggtacaccg atgttcgagc gccggttgcg gctgatcatg gtcctcatgg
    13321 tgatgccggt catcgcggta accgcgaggc tggttcagct tcagatcctc gagggtgagg
    13381 agtatgcaag caaggccgag gcgctgctga tcaagcccat gaggttatat ccctgcctga
    13441 ggggtgagat cgtcgactcg gagggcgtgc ggctggcata tgacgcggaa tcctgggaca
    13501 tttgtgttca ctatgggatt ctctcggaag ccgaggatca tcttcgagcc atggcccgcg
    13561 aacggctggg gagacggggt attcccagga ccgctgacaa cgtagccgag gagatgcaca
    13621 agcttggtat cgagatcaac gaatcatggc atgcgatcgc ccgcctgacg ggcagacagg
    13681 tcgaggagct tgacgctgtt cgggaaagac gcttgcggca agtccagcga atcaaggagg
    13741 tggtcacgcg acggaggaag atcgatacgg tggtcgacga agagcggacc gttcacccga
    13801 tcgtccaggg cctgaaccac cagatgtgcg tcgaggccaa ggtgcaactg gcggagtacc
    13861 cctgggtgga ggtgctggtc agtcacaccc ggcggtacga gggcggcgat gccatggggc
    13921 atctgtatgg tatgctcaac gaagtcggcc ccgacgacct gagagaaaac agcaacaacg
    13981 cagacatact ctctcgatac cttctcggcg atctgatcgg tcgcacgggt ctggagcgac
    14041 ttggagaaca gtggctccgc gggcgcagag gttgcgagca ggaggatctg cgaggcgttc
    14101 ccaccactca gccggtcgcg ccggtggacg gcaacaccat gcggttgagc ctcaacctcc
    14161 gcttgcagca gtactgttat caccggctcc aagcggcggt gcgggaacat cctccgagca
    14221 ctggcggtgc ggccgtgatc ttggatgttc ccacgcgtag agtgctggcc ctggtgagtt
    14281 atcctgcgtt tgacccgaat ttgccggata cggagagggc caagcttgcc gcgcaagacc
    14341 ccttcggcca gcctcacgtt tttcgcgccg ttcatcagcc ttatcccccc ggctcgacga
    14401 tcaaagccat ggttttgccg agcgctttga ctgaaggcaa ggttggacca ggcacgacct
    14461 atgattgcgt cggccatctc tttcccggct tgcccaacaa atggcagtgc gatgcccggc
    14521 gggcacacgg aacggttgac ccggtactgg ctgtccagaa gagctgcaac gtgttcttct
    14581 accacgtggg cgagaacatg gggttggcta acctggtcaa atggatggat cgattcgggt
    14641 tcgggcgggt cacgggcata gggctgattg aggacgcgac cgggacgctg cctcagctca
    14701 ggcgagacat gggtgccggg ccggcgcgcc tggcggccat tggccaaggt gaggtcagcg
    14761 ctacccctct tcagcttgcc aacatgatgg ccacggttgc ctcgggcgtc tggaagccgg
    14821 tgaccatttg gccggacaac cccgaccaat ctttgcaggg ctatgcgctg cccatcccgg
    14881 cggccaactg gaggctggtt cgggaaggtt tgtacaaggt ggtcaacgag cagggcggta
    14941 cggcatacgg cccgcttcgg gcgaagctcg ccggcgaaga agagtttgtc ttgctgggga
    15001 agacgggcag cgccgaggcc ccggctttgg agcggctcta ccgctgtcgc ttcccggacg
    15061 gcaccgagaa gaccatcaaa gcccgaacgt tccgcgaatt acgaaatcgt tatccggaag
    15121 gacagaagcc tgagtacatc agccaagagg acgcccaagt ctacccgacc catggctggt
    15181 ttgtcgggta tctgaccacg cgggggcatt accttgagcc cgcaatcgac ggtgagctta
    15241 acgttgcgat cgcggtgatc gtcgaatacg ccgggcacgg gggtgcggtg gctgccccag
    15301 tggcgcggga catgcttcag gccatgatca ccctgcaccg cggaggaaca atcgaaaccg
    15361 caccggcgga cgattccacc gtccggtcgg cggccgccgt caccgatgcc gccggagagg
    15421 gggagcgacc atgagttcgc tcttcagcac tcgcggaggc tggatcatcc tgctgccggt
    15481 cctgccgctg ctcgtggcgg gggtgttaac catccaggcc accaacgcgt ccgaccagcc
    15541 cggccgactg gcggatgaga cggtcaaaca gattgcctac ggcgcggccg gcatcggctg
    15601 catgatcctc atcgttacca tcggatacct gaggatcggg aggttttcct acatcctgtt
    15661 tggaatcagc atcctggtgc tcgcttgcct ggtcatcgac cggtggtttg accttccttt
    15721 cgtgcccgtg aaacgaaaca cccgccgctg gctccaggtg ggcggcctcc aggttcagcc
    15781 gtcggaaatg atgaaagtcg cgtatgtgtt ggggttggcg tggtacttgc ggtatcgcag
    15841 caactaccgg acgcttctgg gattagtgcc gccgttcatg ctcgcgctgg tgccgatggg
    15901 tctgatcaag ctgcagcccg atttggggac cctgttgctg ttcctgccgg tcctgtttgt
    15961 ggtgctctac gccgcgggcg ccaagggcaa gcacctcgct gcagtcatcg tcatgggctt
    16021 gctgtgctct ccgctgttct ggacgaagat cgagccctac cagcaattgc gaattaccag
    16081 cgtgatgctc cagaacaggg ctgtgcgcga ttatctgagc aagccgatgc cgtcccggcc
    16141 cgacttgccc acccgatggg aatactttcg gcccaaggag ttctcacaaa gccgctggat
    16201 gctggagctg gccaactggg aaaatgtgac tgggtaccaa cttacccgga gcaaaatcgc
    16261 ccttggcagc ggcggcttgt tcgggcaagg ctccggacga ggggtcttcg tccaatacga
    16321 ctttctgccc gagaggcaca acgatttcat attcgcgatg atcggtcacc aatggggtat
    16381 cattgggggt gtactgacac ttttgtgcta cggcctgatc gttgtttttg gatacgagat
    16441 cgccgttttg acccgcgagc cgtttggccg tctggtggcc gtgggtctga cgacgatgct
    16501 ggccttccag gcgatcacca acatctgcat ggcaattggg ctggggccgg taactggcgt
    16561 tccccttccg tttgtcagtg cgggcggcag cagcctggtc atcaacttca tggctctcgg
    16621 attgatcatc agcgtggcac gcgaccgtcc tcttctgttt accaaccggc cgtttgaatt
    16681 cgaaaccgag gaagaggagg attaatgggc ttgtccgcaa ggcgtttgcg gctcgcaaaa
    16741 ccggcggacg tccgatagca tgctactgct gatttgaagg ccccgagggc tcgatggtat
    16801 attcaggtag cacgtaccaa gccgagattg ccgccttggc aatcagaggc tttgctccca
    16861 tcgcttgtgc aagggcccgg tacagaaacg gaagcagaca tggccgacga tcaacttact
    16921 ccggagacgc tgaatgccgc tcaacccaag aacatggccg ttgccgcgaa gatgcgggag
    16981 tttctctctc aggggcgttg ggccccgggc gaccagatcc cgaccgcgcg gcagcttgcc
    17041 cagatgttcg gcgtgagcct ctcgccggtc ctgcaggcac tccagatcct tgagaaagaa
    17101 ggcctgctgc ttcgtcgggc gcgggtgggc acctttgtcg ccgagcgccg ggcgaagaag
    17161 gacttgctcg cattcatcac cgccacgtgc gatcttgagc cctatattca ctcgctgtgg
    17221 ggtttcgagg aggaagcaag ccggcgcggg tatcacatcc tgttctgtaa cgccaatcgc
    17281 gttccagagc gtcttgccca gatggccaat gcctgcattg agcagggagc agcgggcatt
    17341 gccttcaccc cgcctgaggg aagcttctac atggactgga atcgggcttt ctacaagggt
    17401 gtcgccgagc atggcacgcc cctggtcgcc attaccagtc agcccttccc tgaagaggat
    17461 ccggcgatca tctcggccgc cattcctgac ggggtctggc aggggcggcg catggccgcc
    17521 cacctgatcg accagggcca cacccatctg gctttcctgt acgggcgatg gtcctgcacg
    17581 atcgaggatc gcaagcaggg ggtaatcgca gaagccaagg aacgcggttt gccgcccgtc
    17641 gatgtgcgca gcgtctcgcc cgaggagctg ctgcggaccg gcgaggttta tgacgtgacc
    17701 gccatgttag gcgactggct ggggcaggat ccacccatca cggcggtggt gtgctgctcg
    17761 gattatgccg ccagaatcgt ttatgagcac gccagcacgc tgggcatgtc gattcccgag
    17821 gacatcgcgg tggtggggtt cgacgactcg tcgttcgccc cgctgctggg ggacggtggt
    17881 ctgacgacca ttcgcgtgtc gtacattgag atgggccgtt ccgcggcgga gctgttgatt
    17941 gcccagatca acgacccagg tcggccccct cgcatcctga tgtctcgcgg cgagctggtg
    18001 gtacgcaagt cctgtcgatt ccttccttcc caggtggaga gcgatcggag ataggcccgg
    18061 catgcaatag ccagggggta cggcggccgg ctgccccctg atgcctggcc ctgccaagcc
    18121 ggtccgggag gccgtgtccc tcgcgagaag attcttcgac gctcaggtct cggttctcgg
    18181 gcgccggccc gctctcaggc cttgtacacg ttcttctttg gtcccttgac gcgggcgcat
    18241 gcgttgcggg tgtcgaccac caccttcgcg tttcgaacga tcatgccgta gtcgtagcaa
    18301 tcgtgatcgg tagagatgac cacgcagtcg tacttccgga tgttggccgc cgtcaaaggc
    18361 actgatttca ttttgagatc gtgctctcgc tgcttgtggg tggttgggat gtacgggtcg
    18421 ttatagtcga ccttgcagcc gcgtttcttg aagatctcga tcagctcgat cgagggcgat
    18481 tcgcggatgt cgtcgatgtt cttcttgtac gccagaccaa gcacgaggat tttcgacccc
    18541 ttgagtgctt tggcccgttc attgagagcc ctggccgtat gttccaccac gtactccggc
    18601 atcgcggtgt tgatctcgcc ggccagctcg atgaagcggg tggtcactcc gaactggcgg
    18661 gctttccagg tgaggtaaaa cgggtcgatg ggaatgcagt gcccgccgag gcccggtcca
    18721 ggataaaacg cctggaaacc gaacggcttg gttttggccg cctcgatcac ctcccacacg
    18781 tcgatgccca tgcgatgaaa gagcattttg agctcgttga ccatggcgat gttcacgcac
    18841 cgatagacgt tctccaggat cttggcggcc tcagcgacct cgcaactcga aaccggaacg
    18901 atgttcttga tggcctgacg gtaaagggcg acagcaaccc tgaggctggc cggatcgtgg
    18961 ccgccaacca gtttggtgat tgtctcggtg gtgaagtcct tgcggcccgg atcctcgcgc
    19021 tcgggagaga aagcaagata gaaatccttt ccagccttca ggccggtctt ttcgaggatc
    19081 ggcttgagca cctcttttga cgtgccggga taagtggtgc tctccagcga gaccaggtgg
    19141 cctcgccgaa gctgggtcgc aatcgtttcc gtggtcttga tcacgtaagt catgtccgga
    19201 tcgcgcgtct tggacaacgg cgtggggacg cagatgatca gggcgtcaca cccccgcagc
    19261 accttgggat tcgtggtagc ctcaaaacgc ttcgacgatc tcatcgccga tacggcttgg
    19321 tcggggatat gcttgatata gctccttccg gcgttgagca ttttgatctt cctcggatca
    19381 atgtcaaaac ccaccgtcgg cattcccacg ccgcaaaaac tcctggccag cggcaagccc
    19441 acatagccca tccccagaat gccgacacgg gcttgtttgc ggttgatctt gctcagcagt
    19501 ctgctttcaa gcattgaatt actcctgtca gttgtccggt catgaggcaa ggcgtgcacc
    19561 gacgccgggc ggtccgacga ggctgaccgt gccgctcgcc gcgagatgga gaaatcactc
    19621 gaacgtcgca taagctggac cgataatcaa ggtccgggtt cttgagcatc gtctggaata
    19681 tgtcggcgaa aagccccgca cgctataatg gcagcccggt gcgccgaacc gaagttgaac
    19741 caacgtggag gatcggatgg aaatcgcctt atcgcggccg gacatcactc aagcggaacg
    19801 tgacgctgtg ctcgaagtac tccagacacc caatttgtcg cttggtccca agctgcccga
    19861 attcgagagg gtattcgccg aatacgtcgg cagtcggtat gccgtcgcgg tcaacagcgg
    19921 cacgagcgca ctgcacctgt gcatccgtgc gatcggcctc aagcccggcg acgaagtcat
    19981 taccacgccc ttctcgttta ttgcctcctc gaactgcatc cttttcgaag gcggcaaacc
    20041 ggtcttcgtt gacatccatc cggaaacctg gaacatcgat ccggacctga tcgaagccgc
    20101 gatcacgcct cgaacccgtg cgatccttcc cgtagacgtg ttcggtcaca ttgccgacat
    20161 gccgagaatc cgccagatcg ccgacaaata cggcctccgc gtgatcgagg actcttgcga
    20221 ggctctcggg gccgcgctca atggcaagaa agcgggaacg ttcggcgatg ccggcacctt
    20281 cgggttctat ccgaataagc agatgacaac aggcgagggc gggatgatcg tgaccgacga
    20341 cgggcaaatc cgcgatttgg cggtttcgat gaggagccag ggacgcgggt cgggtggcgg
    20401 atggctcgca catgagcgat tgggttacaa ctaccgcctg agtgacatca attgtgcgct
    20461 gggaattgcc cagatgaaac gcgtagacga aatactgtcc cacagactca attccgccaa
    20521 ctactacttg gtccggttga gcgacgaaaa gcggatttcg atgcagaagg cattgcctgg
    20581 aagccaagtc agttggtttg tcatggtcgt tcgtctcaac gacgactact ctgcggaaga
    20641 tcgtgatcag atcctggtca gacttcgcca gcagggcatt ggatgcagca actatttccc
    20701 ggcaatacac ttacagccgt tttacgcccg cgaattcgga tacaagcgcg ggcagtttcc
    20761 catctgtgaa gccctatgcg accgcacggt ggccctgcct ttccacggtt tgctccgcga
    20821 agaagaggtt gacatcgcct gcgaggcctt ccgccggttg ctgtaatcgg acggatcgac
    20881 gccgaatcgt tgatggtttg acagtttagc aggccaattg ctagcatctg ggcccccaag
    20941 ccgggctgag tgtctcctcg tacgattgtg cgcgcgtcat caggcacagg gtgcctgcgg
    21001 cagccggccc gaatgtggtt ctggttgagc aaaggaaagg acgacaggca tggcattgcc
    21061 cacacctctt gccggcgatc cgatccgcaa atccagctcc aagctggaag tgccggatca
    21121 tccggtcatc ccgtttatcg ccggagacgg caccggcccg gacatctggc gggcctcgca
    21181 attggtgttc gatgccgccg ttgccaaggc ctacggcggc aagcgaaaga ttgcctggca
    21241 cgagcttctg gccggggagg aggcatataa gcgaacgcag aactggctac ccgatgagac
    21301 agtcgaggct ttcagaacct atctggtggg catcaaaggc ccgctgacca cgccggtcgg
    21361 cgggggcatt cgttctctca acgtcgccct gcggcagttg ctcgatctgt acgtatgctt
    21421 gcggccggtg cgttacttca aaggtgtgcc ctcgccggtc cgcaaccccg aactcgtgaa
    21481 tatggtgatc tttcgcgaga acacagagga catctatgcg ggcatcgaat tccaggcggg
    21541 cacacccgat gccgagaagt tcgccaagtg gttgcaggag gtctttcccg accggttcag
    21601 gaagattcgt tttccggcta cggccggcta cggcatcaaa ccggtcagcg ccgagggtac
    21661 caagcgtctg gtcaaggcgg ccatcgacta cgcaattgca gaccggcgca aaagcgtgac
    21721 cctcgtccac aaggggaaca tcatgaagtt caccgagggg agcttcaggg actggggtta
    21781 cgagctggcc aaggaggcgt acggcgcaac gcccctggac ggcggacctt ggtgccggct
    21841 gccggcggac aagggcagcg ttctgatcaa ggatgtgatt gccgacgcgt tcctgcagca
    21901 gatcctgacg cggccggcgg agtacgatgt gatcgcaacg cccaacctca atggcgatta
    21961 catctcggat gccctagccg ctcaggtcgg tgggatcggt atcgcccccg gcggcaatat
    22021 caactatgac tcaggccacg ccatcttcga ggccacccac ggtacggcgc ccaagtacgc
    22081 gggtaaggac caggtcaacc ccggcagcct cattctgtcg ggcgagatga tgctgcggta
    22141 catgggctgg acggaggcgg ccgatctgat actcaagggt atggacggcg ccatctccgc
    22201 tcgcaccgtc acttacgatt tccatcgcct gatggtcaat gaaggcgtcg acgccaagct
    22261 gctcaaatgc agtgaattcg gccaggcggt ggttgagcac atgggggagg tctgacatcg
    22321 cgcgtcgcag cgttgggaga cggtccaatt gagggcaaga ggcggtgcgg gtacaacggg
    22381 tgcgagagaa gtgaacaatc ctgaagttgt cagtcggcag agcgggaacc gagaatcagg
    22441 tactgagaag tacttgatat caaaacgcgt atgcgaggcc gtgcggtact cttccatagc
    22501 cagaagtgta cct
//
LOCUS       DAOTPW010000071        22385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2387_length_22385_cov_6.210614, whole genome shotgun sequence.
ACCESSION   DAOTPW010000071 DAOTPW010000000
VERSION     DAOTPW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22385
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2387_length_22385_cov_6.210614"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..3459
                     /locus_tag="P5316_08380"
     CDS             181..3459
                     /locus_tag="P5316_08380"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRR85012.1"
                     /translation="MRRRISRCCWTLPTWMLIFGAVGPASARTVFVASEIDEFRTPPG
                     QTALLLPTAVAVDSAGTVFVADGANDRVVMFNPAGQFMAEIRKVGEESLSRPVGIKTD
                     SAGRLWIADTGHGRLLVRGADGRLDRLITLPPAEGRPPADPTDVLPLAEDRGIWVVDN
                     DGHRLLHLDREGHQLAIVGSRGETPGRFHYPFMIDCNRGQDLLVTDVINGRACVLDGK
                     GHLIGIIGTYGIDMGQLYRPKGIACDSQDRVWISDNLLGVVQIFATDGRFIDVLRDSQ
                     GEPMRFVTPAGLAFDRQGRIYVVEVQPGRVRRLQISEKDEPGTVPTPSRPQFVPEQPQ
                     SCTVCHIEWLQPPGPASATALIKLSLNEAVEPAVSRPDMCLSCHDGSVADSRRLVWQE
                     HGHRSGVEPPASMKVPADLPMVNGKISCRTCHCAHPSGETDDMATAVSLRRANSGNEL
                     CGACHVERTGRPSARVHPVEGVPWAIPEELIEPESRQGPDPRDLTCRVCHMAHGSRED
                     HLLVPGADTGQLCVNCHAKMRPGLWRSDTPREHPQSPPLSDDVQRQAIVAMGGRLGAD
                     DTITCLSCHKMHDGVTDRYLLADTLTDSRLCTRCHPEQGRMADSAHDLRQSAPSERNR
                     LGQTPAQSGPCGPCHSIHQYARRPDPQPADPSGTCTTCHQAGRCAEKVTRLPFGHPLE
                     LAADRATTSKLFTSLSTQPAGSRQLVCVTCHDPHRTDHGRFLVDQADRICGECHEGHV
                     ARLAGGHDFGNKPDLRNARDRGPSETGKCGFCHGIHGTMSPLLWSATGERPENPDEMC
                     LACHADDAQSAAEAAPALRHPSGASTTGKVAWVDSCYPLYDDRCRQSPAGFVTCASCH
                     DVHGDSRDDHALVRGDDPNKSPAACLACHENARAIESTMHSQEFMQRRFAAMDDDRSA
                     RFCAPCHVIHDDTSGPQTVDRFAGAVKELSSDMEPCLECHGAGGHSTLVRVTLHPVGG
                     LRNVAASGQPGFLPLADNQGRLGQEGRVTCQTCHLPHGRAASGGLPPVDASTASEPER
                     RGLKALLRPYVVPNLCSACHGSEGLARFLYYHHPEKRRLLQTNANGLSLDTFEPPSDP
                     SVRR"
     gene            complement(3410..4549)
                     /locus_tag="P5316_08385"
     CDS             complement(3410..4549)
                     /locus_tag="P5316_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85013.1"
                     /translation="MSKSKRMLFRSWRSAALGLAVIGLACVWGGSTCTPEHPVPPFPQ
                     QAWPTSLHATRRGKATFYNAENGGFGTLTGIAIEDLWCQDCHANTRADGTPIDSSTYR
                     PDCSDCHITPGDAVAEIICLKCHDRRRIERNRFTDVHRDQGMTCMDCHTTREMCGDGT
                     PYPSMMEPGAMDVTCEGCHADRPDNTSHSVHASRVHCSSCHAQAVVVCYNCHFQQDPG
                     AGRPTTHVGPVMREFTFLARHATTGQVHTATMVPAVWEDSSFVLITPYASHTITAKGR
                     PCDACHYNQTVRKYAETGRITVTRWDADESQLIQPRGVIPVPPDWRQAMQFDFIEYLR
                     NPDLPEDDPGQWGFLKSGADLMQMMYAEPLTVEQMDRLEVQRYRD"
     gene            complement(4722..5018)
                     /locus_tag="P5316_08390"
     CDS             complement(4722..5018)
                     /locus_tag="P5316_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR85014.1"
                     /translation="MASRKHPGSFLLKLCLTVLAGVLCTAQMCAVSGRDTDGDGVPDA
                     ADLCPTDPDKTAPKICGCGVPETDTDGDGAPDCIDQFPNDPNSIFEQDDCGCGG"
     gene            5860..7152
                     /locus_tag="P5316_08395"
     CDS             5860..7152
                     /locus_tag="P5316_08395"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRR85015.1"
                     /translation="MTGLPSVGTVLVAGLMAMIALAGCDPGIVWPPDDPPNNGDDIPA
                     EYVGAITCAACHPAPGSLHARHGHSHALKVVEGIAPEYPATPGLGEAPNPPAGLDWSD
                     ISFVIGGSAKSALFVDTAGFLLTDGTAGTFTQYNLANPAALLPAGFVPFKPEQVAPHP
                     FGEDCFRCHTTGPESLAMNGGRRQGNLPGIEGTWSQAGVQCEACHGPGSRHLLDPAAG
                     KLTVDSSGGICATCHVNQDEPSEIAVEGKCILGRQQYAEFAASPHQGFSCTFCHNPHA
                     SILRDRENGLRNTCGNCHLDFDTASHEGNVFQWGDYVEAVRCESCHMPLVASNGSCRD
                     VELPRGEIVSIGDTRSHLVHIDTSALDPTDILSADGTRVRLDEQGRAGVPVSCVCQRC
                     HHGLGNAFTLTAAEAGEIGRRLHSTEMTEAVTAGRHRN"
     gene            complement(7339..7426)
                     /locus_tag="P5316_08400"
     tRNA            complement(7339..7426)
                     /locus_tag="P5316_08400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7390..7392),aa:Ser,seq:gga)
     gene            complement(7651..8730)
                     /locus_tag="P5316_08405"
     CDS             complement(7651..8730)
                     /locus_tag="P5316_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HRR85016.1"
                     /translation="MTKHENYRRYGDASPLPRRQFASDNYAPVCPEAWESMAAANRGH
                     APSYGEDAWTRKACDLIREAFETDCEVFFVFNGTAANSLTIATLCKPYHSVICQDMAH
                     VETDECGAPEFFSSGSKVLHVPGRDGKLDPAGVEHTVKRRSDIHYPKPHALSITQATE
                     LGTVYSVEELCGLGDLARTLGLKIHMDGARLANALVSLAVSPKEITWKVGVDVLCLGA
                     TKNGGCLGEAVVFFDRSLADEFDYRCKQAGQLASKMRFLAAHWIGLLEHGTWLRHAEN
                     ANRCARRLEEQLRRIPGVRIMFPCQANSVFVEMSPAMVEALHQRGWHFYTFIGCGGAR
                     LMCSWDTTDEDVDAFVADAREVAAQ"
     gene            complement(9070..10020)
                     /locus_tag="P5316_08410"
     CDS             complement(9070..10020)
                     /locus_tag="P5316_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85017.1"
                     /translation="MRRKNRCGFTLIELLVVVSIIALLIAILLPSLTMARELARRAAC
                     AANLSGIGKGFYTYAMDNGDSCPIAAPNSTMNPNPSPVEYYNMTGKNGGAGTLGDPGR
                     QPSGSYWAQLSTTRTFWLLVKINGTSSRSFICPSSGDAPDNAENPADYWDFMAASPGT
                     DQHRGWAANRNNETHVSYGTQVPYGSKGRPGIEADPRMALAADKGPYGGVSLGNPQII
                     APPPGIDLRNVSPDECMPYNSGNHGGLGSGQGQVVLFVDSHAEFLNKPVVGAAMDNIY
                     TAWNLNGNQPEPTSRYCGRRPSADLPSLVPAADSDTLIYP"
     gene            complement(10745..11737)
                     /gene="xerC"
                     /locus_tag="P5316_08415"
     CDS             complement(10745..11737)
                     /gene="xerC"
                     /locus_tag="P5316_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRR85018.1"
                     /translation="MSETTLIDEFLNYLRFERHFSPHTAKCYAADLHQFVEFLGGPAD
                     FQGSTPPSTTTGQPPAGGAAPAAPTRAQVDTREVLKGVQTEDVRRFLAFLTDREYSKS
                     TTARKLATLRSFYKFCLKRGYVSANPLSAIRTPKQEKRLPKFLEEDEVRKLLETPDVS
                     TLLGARDRAMLETMYSTGVRVSELVDLNIEDVDTANECLHIHGKGKKERLTPISPTAL
                     AWIKHYMDMRRADPRASTFDQKPVFVNKHGKRLSTRSVRRKLDKYLSICGLDPSISPH
                     TLRHSFATHMLNRGADLRAVQELLGHQSLSTTQIYTHVTTSRMKQAYDNAHPRA"
     gene            complement(11818..12024)
                     /locus_tag="P5316_08420"
     CDS             complement(11818..12024)
                     /locus_tag="P5316_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85019.1"
                     /translation="MNHVRQLSLVKGNHRYVFRYKAGEEPSIIAVFASMAADPEHDFD
                     WFDAAVLSYQMGRRLEMDLEKTPA"
     gene            complement(12021..12692)
                     /locus_tag="P5316_08425"
     CDS             complement(12021..12692)
                     /locus_tag="P5316_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85020.1"
                     /translation="MEQEAEPALYLPEEVRIAVAPALNHSGGAEFDPARVGDLMASEL
                     SGLEGVKVVGVSRVLAVLAEQGDVRIRSPEHALEVCERLGVDAILVFAVTEYDAYTPV
                     VGLAAQLYGPWRPEWPQGGLDPVAASRAARPFPVPQGFGGIRPQAQVQRTFNAAHEAV
                     QRDVQRYAHSRSATAGPLGWKRYLASQEAYLRYCCFTLARELLLQRMENKSAAAVAAA
                     GETGQ"
     gene            complement(13033..14229)
                     /locus_tag="P5316_08430"
     CDS             complement(13033..14229)
                     /locus_tag="P5316_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRR85021.1"
                     /translation="MPINTRARMVRRGRGAVCAAIITTCLVGCTCFEKAKEESERPLP
                     PPPPEPAMPAAQDAQITLFGELPELARLPFHPRANAPMKQHSFTAEGADFDVDVSPDG
                     KNLVFASTRHSTQADLYMKTVDGVAVTQLTSDPAPDVQPCFSPDGKSIVFASNRSGNW
                     DLWLIGTDNGQATQLTRSPWHEIHPSFSPDGRQVVYCMFNVRAEQWELWILQLDRPDA
                     RRMIGVGLFPRWSPKGDSILYQRARQRGGRWFSIWRIDLQDGEPRFPIELAASSQMAF
                     IQPTWSPDGDWVAYGTALPQPVPADPAAGPLPPASRGDIWIMRADGTGPRQLTDGQGA
                     HFGPCWAADGRVYFTSRQSGSENIWSVLAEAVAMPQTPLAADDSAAAPAGAHSAVVPA
                     GNPRGG"
     gene            14724..16634
                     /locus_tag="P5316_08435"
     CDS             14724..16634
                     /locus_tag="P5316_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA strand exchange inhibitor protein"
                     /protein_id="HRR85022.1"
                     /translation="MDTHTLEKIDFDRVRQILAEHASCALGRAMVSKVRPVVREDLVR
                     QWLRQIEEMIEASATIGLPPFGGVRDVREIVRSAIPPHLPEPAEFATVAETLEATHRI
                     VRWGDKLSSASGELRRVCDKIGDFQAIADVIHRVIDGSGEVRDDASAKLRAIRTEIAN
                     ARISIGHVFDRILRDRHVTRWLRYANATFHEDRLVLPLAAEHRGRVPGIVHRSSDSGA
                     TLFVEPAEAVELNNRIITLKNDEQTEISRLLWNLTHQLHLNSVEILKTLDALAILDLI
                     AAKARFARSYDLHIPQISNDGSLRLRQARHLLLVEMQKQAAGRGEKREVVPIDIRLGD
                     DFDILIITGPNTGGKTVVLKTVALACTMAQAGLPIPAAEGSRVPIYHDILVDIGDEQS
                     LQQSLSTFSSHISRVMAMIKHARPGTLMLIDELGAGTDPEEGAALGRAIVAELLKRKC
                     PALVTTHLGVLKSLAYTEPRAENACVDFDAQTLRPTYRLLIGEPGNSNAINIASRLGL
                     PPHIVKAARAFLADSHQQLSRAIAGTLQSRRLAELARQQSEAARVEAEKQQAAAENAR
                     RELEEQQARFERWVAMILSLQPGHRVLVRRFDREGTIVRMLLHKQMAVVSVGAMEMEV
                     PIKELLPVTDDH"
     assembly_gap    16920..17019
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17065..18189)
                     /locus_tag="P5316_08440"
     CDS             complement(17065..18189)
                     /locus_tag="P5316_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HRR85023.1"
                     /translation="MKTDRTAKLPPFFTHPIGSLPRPKALLSVLDRRKQMDPGRLARV
                     MDDFVLFAIRLQEEAGMDVVSDGEWRREQYIRELLGRIGGFEKCRRYEHQGEVKYTDV
                     VVRRIAATEPVFAEDAAFLAGHTERVTKFALPSPFLIALRYWHADHSREAYPTMEHFL
                     EHLTEILAREARALVQAGIDIVQIDDPALVYFCDRDLMAGISSHDERLRRAWNPEKEL
                     PMAIAAINRVVENLRAETHLHCCHSVYKRRSDVTGNYKPLLPAFKDLKVDRVNLEFAY
                     KDTGDISDLKLLPSHLSVGMGVVDVRGEQIPTVEQIESLAAAGAEILSPNRIALNPDC
                     GFAPEAVEPPTIDEAFEKLSHLVAAATRLRERLSSTKPRV"
     gene            18323..19771
                     /locus_tag="P5316_08445"
     CDS             18323..19771
                     /locus_tag="P5316_08445"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRR85024.1"
                     /translation="MNSVFLVRRTRLRHALFGLMLIAVCAPAVFGKAGPAFKLVGKEV
                     FIPGRPGVRVVAQAFYVRSEGQEMISRYSEQTKSDKADIAYQRFSHDNGRTWSTPAML
                     VTNERVSGGAVGPTPSSAHSVSAPVTNESAPGGTRRRAMKPGFVDPTRDVLVTVFQEA
                     ILPTDNPLEGMKYWTLHYALSRDGGKTNYHEGPIVHKGTEYSHEHPLPNVWVGKNSAM
                     IGDTACVPIRIRTGEILQPIQITPIGPDGEYYNPGGGYTYHDSAVLIARWNDSGILDW
                     ELSKLVKGDATRSTRGMLEPTIIEAPDGRILMVLRGSNDRKPELPGYRWYAVSTDHGR
                     SWSPPKPWTYDNGESFHSPSSCSQLLGHSSGRICWIGNISPSNPRGNSPRYPLVIGEV
                     DPESLLLRKDSVLVIDDRLPGEPEWTALSNFYAREDRISREIVVHLSPIGKGLAQSSR
                     PVVQATQPTFEWTADAWVYRIAVLMAEPRKRE"
     gene            complement(19832..20857)
                     /locus_tag="P5316_08450"
     CDS             complement(19832..20857)
                     /locus_tag="P5316_08450"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRR85025.1"
                     /translation="MVEKIIIIGSGPAAWTAAIYAARANLKPLVFAGRPKQTPSTVLP
                     GGQLMLTTEIENFPGFPERITGPQLMARMEQQAVRFGARIQTDNGQNPDVSNPDDGHS
                     YSYHDVTKVDLSARPFKVWGEDATMIETHSLIVATGATANWLGLPNEQRLARSGGGVS
                     ACAVCDGALPVFRNKELAVVGGGDSAVEEATYLTKFASKVFMIHRRDQLRASKIMQKR
                     VFDNPKIEVLWNKIPVDVLGDKLITGVRLKDTKTGEVSDLPVGGLFVAIGHTPATAFL
                     EGQLELDQKGYVKLKDAYRTTTSVDGVFAAGDVVDSVYRQAITAAGMGCKAAIDAERW
                     LAEQGIE"
     gene            21277..22173
                     /locus_tag="P5316_08455"
     CDS             21277..22173
                     /locus_tag="P5316_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRR85026.1"
                     /translation="MRIALFFADGRAYFLGIALVIIGLLVLAAGKKRKPSRRIRIVLA
                     ARVLLIMGVLFILLSATPAPIWFYGLWFITIVAWLIHQSEPMLKREVGIALRIGSLLF
                     CLAAAGMEIPHRLLPKLEPQGQEKVYVIGDSLSAGMGTEEMTWPKVLAAGVPVEIVDL
                     SAAGATAGTAMEQAKRVPPGPAIIVIEIGGNDLLGGTPGHVFENDLRRLLETLSGDSR
                     RLVMFELPLPPFFNKYGRIQRRLASEFDVALIPKRILSDVIGSARGTLDGLHLSDDGH
                     RQIAETVRRVVNLPEPRGVPAS"
BASE COUNT         4042 a         6887 c         7124 g         4232 t
ORIGIN      
        1 ctgcctcttg gaaaacgtat ttggtccacg cgagacgtct gcaccggcct gtggagatac
       61 agcccgcaac cgcgaatgct gcacaaccga cgtgacacgc gacgtcgcga cgcccattat
      121 tactgaggtt catcttcaag acaacggcct tgagaacggc agttggggat cgggatggaa
      181 ttgaggcgac gcatatctcg ctgttgttgg acgttgccga cctggatgct gatcttcggg
      241 gcggttgggc cggcgtcggc gcggaccgtc ttcgttgcaa gcgagatcga cgagttccgg
      301 acgccgcccg gccagacggc tctgctgctg cccaccgccg tggcggtgga ctcagccggt
      361 accgtgttcg tagccgacgg ggccaatgac cgcgtcgtta tgttcaaccc cgcagggcag
      421 ttcatggcgg agattcggaa ggttggcgag gagtcgttgt cccggccggt aggaatcaag
      481 accgactcag cgggtcgatt atggatcgcg gacaccggcc atggccgctt gctcgtcagg
      541 ggagccgatg gtcgccttga tcgactgatc accctgcccc cggcggaagg caggcctccg
      601 gcagacccga cggatgtgtt gccgctagcc gaggacagag gcatctgggt tgtggacaac
      661 gacggtcacc ggttgctgca cctggatcgc gaaggccatc agctagcgat tgtgggatcg
      721 cgaggcgaga cgccgggtcg ctttcactac ccgttcatga tcgactgcaa ccggggccag
      781 gatctgctgg ttactgatgt gatcaatggt cgagcgtgcg tcctggacgg caaagggcat
      841 ctgatcggca ttattggtac ctacggcatt gatatgggcc agttgtaccg gcctaagggc
      901 attgcctgcg actctcagga cagagtctgg atcagtgaca acctgctcgg cgtcgtgcag
      961 atcttcgcga ccgacggtcg cttcatcgac gttctgcgcg attctcaagg cgagccgatg
     1021 cgctttgtca ctccggcagg actcgcgttt gaccggcaag gccgaatcta cgtcgttgaa
     1081 gttcaacccg gtcgggtcag gcggcttcaa atcagcgaga aggacgagcc tggcactgta
     1141 ccgacgccgt cgcgccccca atttgtgccc gagcaacctc agtcgtgcac ggtatgtcac
     1201 attgagtggc tgcagccgcc ggggccggcg tcagccacgg ctctgatcaa gctctcgctg
     1261 aatgaggctg tggagccggc tgtcagccga cccgacatgt gcctctcgtg tcacgatggg
     1321 tccgtcgccg attcgcgccg ccttgtctgg caggaacacg gtcatcggtc gggagtggag
     1381 ccgccggcat caatgaaggt cccggccgac cttccgatgg tcaacgggaa gatctcgtgc
     1441 cggacgtgtc attgcgccca cccgtccggc gagacggatg acatggccac ggccgtttcc
     1501 ctgcgaagag ccaactccgg caacgagctg tgtggtgcgt gccatgtcga aaggaccggc
     1561 aggccaagcg ctcgtgttca tcctgtggaa ggcgtgccgt gggccattcc cgaggagctg
     1621 atcgagcccg aatctcgcca agggcccgat ccgcgtgacc tcacatgtcg tgtctgccac
     1681 atggcccacg gttcgcgcga ggatcatctt cttgtgccgg gcgccgacac cggtcagctt
     1741 tgtgtgaatt gtcatgccaa aatgcgacct ggtttgtgga ggagcgacac tcctcgcgag
     1801 catccacaat cgccgccgct ctcggacgac gtgcagcgcc aggcaatcgt cgccatgggg
     1861 ggacggcttg gagcagacga cacaatcacc tgtctgtcgt gccacaagat gcacgacggc
     1921 gtcaccgatc ggtacttgct cgccgacacg ctgaccgaca gccgcttgtg tacccgctgt
     1981 caccccgagc agggacggat ggcagactcg gctcacgatc ttcggcagag tgccccatcg
     2041 gagcgcaacc ggcttggtca gacgcctgct caaagcgggc cctgtggtcc ctgccacagc
     2101 atccatcaat atgcccgacg ccctgaccct cagccggctg atccttcagg tacctgcact
     2161 acctgtcacc aggcgggacg ctgcgcagag aaggtcacgc gccttccgtt cgggcacccg
     2221 ctggagttgg ctgcggatcg cgctacgacc agcaagctgt tcacttcatt gagtacccaa
     2281 ccagccggtt cccgccaact ggtctgcgtg acctgccatg atccgcatcg gacggaccac
     2341 ggccgttttc tggtcgatca agccgatcga atctgcggcg agtgccacga aggccacgtt
     2401 gccaggttgg ccggcggcca tgacttcggg aacaagccgg atttgaggaa tgcccgcgac
     2461 cgtggacctt ctgagaccgg caagtgcggc ttctgccacg gtattcacgg cacgatgagc
     2521 ccgctgctct ggtcggcgac cggtgagcgc ccggagaatc ctgatgagat gtgtctggcc
     2581 tgtcacgcgg atgacgcaca atcggctgct gaagccgcgc ctgcgcttcg acatccaagc
     2641 ggcgcatcga ccacaggaaa ggtcgcttgg gtcgattcat gctatcctct ctatgatgac
     2701 cggtgcaggc aatcgccggc tggttttgtg acctgcgcca gttgccacga tgtgcacggc
     2761 gattcgcgtg acgaccatgc cttggtacga ggcgacgatc cgaacaagtc gcccgcggca
     2821 tgtcttgcat gccacgaaaa cgcccgggcc atcgagagca ccatgcactc ccaggagttc
     2881 atgcagcgtc gcttcgccgc tatggacgac gatcgctcgg cgcgattctg cgctccctgc
     2941 cacgtgatac atgacgacac gtcaggcccg caaacggtcg accgattcgc cggggcggtc
     3001 aaggagcttt cgtcggacat ggagccgtgt ctggaatgcc acggtgcggg cggtcacagc
     3061 acgcttgtcc gcgtgacatt gcatcccgtc ggcggcctgc ggaacgtggc ggcaagcgga
     3121 caaccgggtt tcctgcctct ggcagacaac caaggacgct tgggacaaga gggccgggtc
     3181 acctgccaga cctgtcatct gccgcatggc cgagcggcgt cgggaggtct gccgcccgtc
     3241 gatgcgtcta cggcgagtga acccgagcgt cgcgggctga aggcgttgct gcggccttac
     3301 gtcgtcccca atctgtgcag cgcctgtcat ggcagtgaag ggcttgcccg cttcctgtac
     3361 taccaccatc cggaaaagcg ccggctgctc cagacgaacg cgaatggcct cagtctcgat
     3421 acctttgaac ctccaagcga tccatctgtt cgacggtgag gggctcggcg tacatcatct
     3481 gcatgaggtc ggcaccggat ttgagaaaac cccattggcc cggatcatcc tccggcaggt
     3541 cagggttgcg caagtactcg atgaaatcga actgcattgc ctgtcgccag tcgggcggga
     3601 cggggatcac gccccgtggc tggatgagtt gtgattcgtc ggcatcccag cgggtcaccg
     3661 tgatccggcc tgtctccgcg tatttccgca cggtctggtt gtagtggcac gcgtcacagg
     3721 gtcgtccctt ggccgtgatg gtatgagagg catagggcgt tatcagaacg aaggacgagt
     3781 cttcccagac ggcgggtacc atggttgcgg tgtgcacctg gccggtggtc gcgtgccgag
     3841 cgaggaatgt aaactcgcgc atgaccggcc ccacatgagt cgtcggccgc cctgccccgg
     3901 gatcctgctg aaagtgacag ttgtagcaga cgacgaccgc ctgggcatgg caggaggagc
     3961 agtgtacccg tgaggcgtgg acggaatggg aggtattgtc tggtcgatcc gcgtggcatc
     4021 cttcgcaggt aacgtccatg gcccccggtt ccatcattga cggataaggc gtcccgtcgc
     4081 cgcacatctc gcgggtggta tggcaatcca tgcaagtcat gccctggtcc cggtggacat
     4141 cggtgaagcg gtttcgttcg attctacggc ggtcgtggca tttgaggcag atgatttccg
     4201 ctacggcatc gccgggggtg atgtggcaat cggagcagtc gggccgatag gtactactgt
     4261 caattggcgt tccgtccgcc cgggtgttcg cgtgacagtc ctggcaccag aggtcttcga
     4321 tggcaatacc ggtcagcgtt ccgaaaccgc cgttttcggc gttgtagaat gtggccttgc
     4381 cacggcgtgt ggcatgcaga ctcgtgggcc aggcctgctg cggaaacgga gggacaggat
     4441 gttcaggggt gcaggtactg cctccccaaa cacaggcgag gccaatcacc gcaagtccaa
     4501 gggccgcgct ccgccaggat cggaacaaca tgcgtttgga cttgctcata cgccgctttt
     4561 cagctcctcc ggtcgccctc ggtcttgttg cggctcccgc cgattcgaaa ccattcgtga
     4621 acccggccac attgttgccg tcgccgttcg tacgcgcccg gtccattcac caaaggtgac
     4681 cgtcaggcct ctgccaacag ggcccggtgt tcgctttctg ctcacccgcc acacccacaa
     4741 tcgtcttgtt cgaagatgct gttggggtca ttcgggaact ggtcgatgca gtccggggct
     4801 ccgtcaccgt cggtatcggt ttccggtaca ccacagccac aaatcttcgg ggcagtcttg
     4861 tccggatccg tgggacaaag gtctgcggcg tctggcacgc cgtccccgtc ggtgtcgcgc
     4921 ccactgactg cgcacatctg tgctgtgcac aaaacacccg caagcacggt gaggcagagc
     4981 ttcagaagaa agctgccggg gtgttttctc gatgccactg agactcctcc tttcgatctc
     5041 ttgggcgggt caacgtctgc cgcccgcgaa agggtccggc cggtatcact tgttatctga
     5101 gccgccgcac ccacaatcat catcgtcatc atcaacgtcg tgataatggt agatacttag
     5161 aggatcgtcg gggaattggt cgatgcaatc cggtcctccg tctccatcgg tatcggtctc
     5221 tgggctcccg cagccacaaa agccgggggc cctcttgttc ggatccaaag ggcaccgatc
     5281 aacactgtcc ggccagccgt ctgcgtcgac gtcgacatcg ctgacgacac acatttgtgc
     5341 cgggcacaag accgccgcgg caacaacaag acaggttttc gccacaacag cgcgcagacg
     5401 aatgaagaaa accatcggtg cttttccttc cgactgtttt ggcggatcaa cagccgcccc
     5461 gtccggctgc cccccgagtc gcgtaagcgc atccgctgca ttccaatatg ccatgatacg
     5521 cgatttctca accggatgac aagcccaatg caccggtcgc gaatcattcg tcgaaaaccg
     5581 ccacggtgaa ggttgcagcg aggctgtggg gcaagcggcg aataagcacc ggttgaaacc
     5641 agtgttgcag taggcgtttc ggttcaaact tggtaacatg ccagtgtttt gacgtccatg
     5701 gggcccttca cccggccata gccattcaca aggtcgatac tgctcagaac tattaccgcg
     5761 actttgccat cgtgtgtgac actgtttatg ctgttgggtg tgactgttgg ggtgtcgatg
     5821 tggaaaacgc ccatcaggtc aagtcatctg ggtggtggta tgaccggtct cccttcggtt
     5881 ggcacggtgc ttgtcgccgg tttaatggcg atgattgcgc ttgccgggtg cgacccgggc
     5941 atcgtctggc ccccggacga tcccccaaac aacggcgacg atattccggc ggagtacgtc
     6001 ggcgccataa cctgcgcggc ctgtcacccg gctccagggt cgctccatgc ccgacatggt
     6061 cacagccacg ccctgaaggt ggtcgagggc atcgctcccg aataccccgc tacgccgggc
     6121 ttgggcgagg ctccgaatcc gcccgcgggt ctcgactggt ccgatatcag tttcgtcatc
     6181 ggcgggtccg ccaagtcggc cctcttcgtc gataccgcgg gcttcctcct gacggacggt
     6241 accgcaggta cgttcacaca atacaatctg gccaaccccg ccgctctgtt gcccgcgggt
     6301 ttcgtcccat tcaagccgga gcaggttgcg ccccatcctt tcggcgaaga ctgttttcga
     6361 tgccatacta ccggtcccga atcactggcc atgaacggag gccggcggca gggcaacctt
     6421 ccgggaatcg aaggaacatg gtcgcaagcc ggcgtgcagt gcgaggcctg ccacgggccg
     6481 ggcagccgtc atctcctgga cccggccgcc gggaaactca cggtggactc cagcggcggc
     6541 atctgcgcga cgtgtcatgt gaaccaggac gagccttccg agatcgcggt cgaaggcaaa
     6601 tgcatccttg gccgccagca gtacgcggag tttgccgcca gtccccacca aggcttctcc
     6661 tgcacattct gccacaaccc gcacgcctcc attctgcgcg atcgggaaaa cggattgcga
     6721 aacacgtgcg gcaactgcca cctcgatttc gacacggcgt cccatgaagg caacgtgttc
     6781 cagtggggcg actacgttga ggccgtgcga tgcgagagtt gccacatgcc gctcgtggcg
     6841 agcaatgggt cgtgccgcga cgtcgagctg ccgaggggcg aaatcgtctc catcggcgat
     6901 acgcgaagtc acttggtgca tatcgatacg agtgcccttg atccgacgga tatactttcc
     6961 gctgacggta cacgggtccg cttggatgaa caaggccgcg cgggcgtgcc ggtgtcttgc
     7021 gtgtgccagc gatgccatca cggcctgggg aacgcattca cgctgacggc ggccgaggcc
     7081 ggcgagattg gccggcgtct gcacagcaca gagatgacgg aagcggttac tgccggacgg
     7141 catcgcaact gagggcacaa cccggagcaa gcgagggaat cggctgtctc aacatgcttg
     7201 gggcccatcc tgctgccttg tccaacacat cgggcagagg aaaagcggcc gtgcggcaaa
     7261 cggaggctat aaacgcttgc agattccacg cgcagcgtca cctgcaatac gcaaagccca
     7321 ctcttgaagg gagatcaacg gagagggggg gattcgaacc cccggtacag acaagccgta
     7381 cacagccttt ccaaggctgc ccgatcagcc actccggcac ctctccaagc tgctgattcc
     7441 aatggactta cggttccacg gcctgcgggt tctggccgtt gggccggttc attggagggc
     7501 cgccggccgc tctgaaggcg gcgatcggcg gcactaaggg accaaggtat cgacggagga
     7561 tcgggcggtc aagacgatcg tccggcacct cggccggtgc gcggatcgtg acctgccagc
     7621 cgggcgggtg agccgaggat ggcatcggcc ctactgtgcc gcgacttcgc gagcgtcggc
     7681 aacgaaggcg tcgacgtctt catccgttgt gtcccacgaa cacatcaggc gggcgccgcc
     7741 gcagccgatg aaggtgtaga aatgccagcc gcgttggtgg agcgcctcga ccatggcagg
     7801 cgacatctcg acgaagacgg aattcgcctg gcagggaaac ataatgcgca cgcccggtat
     7861 ccgccgaagc tgctcctcca agcggcgggc gcagcgattg gcattttcag catgtcgcag
     7921 ccaagtcccg tgttccagca ggccaatcca gtgcgctgcc agaaaacgca tcttggaggc
     7981 aagctggccg gcctgcttgc accgatagtc gaactcatcc gcaaggctgc gatcgaagaa
     8041 gaccactgct tcgcccaagc atccgccgtt cttggtggcg cccaggcaaa gcacgtccac
     8101 gccgaccttc caggtgattt ccttcgggct gaccgcaagg ctcacgagcg cattggccag
     8161 acgggctccg tccatgtgaa tcttcaggcc caatgttcgg gccaagtcgc cgagcccgca
     8221 cagttcctcg accgaataga ccgtacccag ttcggtggcc tgggtgatgc tgagagcgtg
     8281 cggcttggga tagtggatgt cgcttcgccg cttgacggtg tgctcgacgc cggcgggatc
     8341 gagctttccg tccctgccgg ggacgtgcag caccttgctg ccgctggaga agaactcggg
     8401 cgcgccgcac tcgtcggtct cgacgtgggc catgtcctgg cagatgacgc tgtggtacgg
     8461 cttgcagaga gtggcgatgg tcagcgagtt ggctgccgta ccgttaaaca cgaaaaagac
     8521 ctcgcaatcg gtttcgaagg cctcgcggat cagatcgcag gccttgcgcg tccacgcgtc
     8581 ctcaccatac gagggggcgt ggcctcggtt ggccgcggcc atcgactccc aagcctcagg
     8641 gcaaacaggg gcatagttgt cactggcgaa ctggcgacgc ggtagcggag atgcgtcgcc
     8701 gtaacgccgg tagttctcgt gcttggtcac tgaaaaagat ctccaaaaac tgcggcaaat
     8761 ggaaacgcca cacaatcccg gctgcgtttc agccaacacg gggacatcgg aaggtcgacg
     8821 atacgttgcc ctcggggaaa ccgcggttgc gggcaatgcc gtccggacag cggcgtcgag
     8881 cgagccggcc ccggttttcg tcgccagctt agatatcggt tcacgtgatg gcaataccgg
     8941 ctgagcatgc tcgggcaaat gcaggacagc catctgccgg gccgggcagc cggcgggtgg
     9001 ctggttgccc ccgccggcca tagcccgacc gcccgagccg accctcccgg ggggtgatgt
     9061 cggcccggat cacgggtaga tcagcgtgtc gctgtccgct gccggaacga gcgagggcag
     9121 atcagccgat ggacgcctgc cgcaatagcg cgacgtgggc tcaggctggt tcccgttcag
     9181 gttccaggcg gtgtaaatgt tgtccattgc cgcaccgacg accggcttgt tcagaaactc
     9241 ggcgtggctg tcgacgaaca gcaccacttg cccctggccg ctgcccaggc cgccatggtt
     9301 gccggagttg tacggcatgc actcgtcggg actgacattc cgcaaatcga tacccggcgg
     9361 cggagcaatg atctggggat tacccagcga tacgccgccg tatgggccct tgtcggcggc
     9421 aagggccatc cgcgggtcgg cttcgatgcc cggcctgcct ttggagccgt atggaacctg
     9481 tgtgccgtag ctgacatggg tctcgttgtt ccggttggca gcccacccgc ggtgctggtc
     9541 cgtcccgggg cttgcagcca tgaaatccca atagtcggcc gggttctcgg cgttgtcggg
     9601 cgcgtcgccg ctggacgggc agatgaagga acgggacgag gtaccgttga tcttcaccag
     9661 cagccagaag gtccgagtcg tggacaactg ggcccaatag ctgccgctgg gctgccggcc
     9721 ggggtcgccg agagttccgg cgccgccgtt cttgccggtc atgttgtagt actccaccgg
     9781 agacgggttg ggattcatcg tcgagttagg ggcggcaatc ggacaagaat ccccgttgtc
     9841 catcgcgtag gtgtagaaac ctttgccgat cccgctcaga ttggccgcgc aggctgcccg
     9901 cctggccaat tcgcgggcca tggtcaggct cggcagcaga atcgcgatca acagggcgat
     9961 aatcgacacc accaccagca gttcaatgag agtgaaacca catcggttct tgcgcctcat
    10021 gatgacacct cctgtgcttt cgcacatgct gttcgcgcct tcggcttgtc cggtgcatca
    10081 tgaggttgcc gcttgatggc ggaagggatc gaatcgggta tgagaaatat gagaacgggc
    10141 tgcctggaca ttgtcgatcg cagggtccag ctcttccgcc gtctctcggc cactcgtgct
    10201 gcatgatgac aacctccggc gccgccgccg gccgaggctg ccggaccgac ggctgagacc
    10261 acgagttatc cgcagacgaa ccggcgacgt tcgccggtcg ttgcacgctc cggtgacgtt
    10321 cgggcaggcg cgcggaccct ggtcgggcac acggcacggc cctgaccgaa cggctgagac
    10381 gaacctgtgc tcttgtcctt ttgttgcggt caccccggcc cgcaatgcgg gcgatgcggt
    10441 gtggccgcga ttcgaatggc tgcggacggg gatgtatgag tcgtcgggcg ggaagatgaa
    10501 tgtatgatga ctattattcc actgacgact accctgcgtg caatggctta tgaatcatcg
    10561 agttacggct cacgccgaag ctccttatgt atgagcgacg tatccacagc cactctaccc
    10621 aatctaatgc tttcagatgc tgaaaagtca actggaaaac aggagaggca cccggacaag
    10681 gggtgcctct cggtcgaagc tgtcgttggt tttcaaacgc agtgctattg ccgtcagtgc
    10741 cgtttcaagc cctcggatga gcgttgtcgt atgcctgttt cattcggctg gtggtcacgt
    10801 gggtgtagat ctgggtggtg gacaaagact gatgaccgag cagctcctgg accgcgcgca
    10861 ggtcggcgcc gcggttaagc atgtgggtcg cgaagctgtg ccgcagcgtg tgggggctga
    10921 tggacgggtc caggccgcag atggacaggt acttgtcgag tttgcggcgc acgcttcgag
    10981 tgctgaggcg tttcccgtgc ttgttgacga acacgggctt ctggtcgaag gtcgacgctc
    11041 gcgggtcggc ccggcgcatg tccatatagt gtttgatcca ggccagggcg gtgggcgaga
    11101 tcggcgtgag ccgctctttc tttcccttgc cgtggatgtg caagcactcg ttggcggtat
    11161 ccacatcctc gatattgagg tcgaccagtt cgcttacgcg aaccccggtg gagtacatgg
    11221 tctccagcat ggcgcggtcg cgtgcgccaa gcagcgtgga cacgtcgggg gtctcaagga
    11281 gcttgcggac ctcgtcctcc tcgaggaact tcggcaggcg cttctcctgc ttcggcgtac
    11341 ggatggccga aagcgggttg gccgagacat aaccgcgctt taggcagaac ttgtagaagc
    11401 tgcgcaaggt ggccagcttc ctggcggtcg tgctctttga gtactcgcgg tcggtcagga
    11461 aggccaggaa cctccgcacg tcctcggtct ggacgccttt gaggacttca cgggtgtcaa
    11521 cctgggcgcg tgtcggcgcg gcaggggctg ctccgccggc cggaggctgc ccagtcgttg
    11581 tggagggggg ggttgagccc tgaaagtccg ccgggccgcc aaggaactcc acgaactggt
    11641 gcagatcggc tgcgtagcat ttggcggtat gcggagagaa gtgccgctca aaacgaaggt
    11701 aattaaggaa ttcatcgatc aaggtcgttt cactcatgac tttctagcac cggcggcgat
    11761 tgcctgcgtt tatcaacctg gctctgtggc cgttcgctcg catccgaacc agcgacctca
    11821 agccggcgtt ttctcgagat ccatctcgag tcttcgaccc atctggtacg aaagaacggc
    11881 agcgtcgaac caatcgaaat cgtgttccgg gtccgccgcc atcgaggcga acaccgcgat
    11941 gatggacggc tcctccccgg ccttgtagcg gaacacgtac cggtggttgc ctttcaccaa
    12001 cgagagctgc cgaacatggt tcattgtccc gtctccccgg cggcggcaac ggccgctgcc
    12061 gacttgttct ccatcctctg caacaacaat tcgcgggcca gggtgaagca gcaatatctc
    12121 aggtacgcct cctggctcgc gaggtatctt ttccatccga gcgggcctgc ggttgccgac
    12181 cgtgaatggg cgtagcgctg gacgtcgcgc tggacggctt catgagcggc gttgaacgtg
    12241 cgctgcacct gggcttgcgg tcggatgccc ccgaaaccct gtggcaccgg aaaagggcgg
    12301 gccgcgcggg atgcggcaac ggggtccagt ccgccttgcg gccactcggg ccgccacggc
    12361 ccgtaaagct gagccgccag gccgaccacg ggggtgtacg cgtcgtactc ggtaaccgcg
    12421 aagaccagaa tcgcgtcgac tccgagtcgc tcgcacacct ccaacgcatg ttcgggagac
    12481 cggatccgga catcaccctg ctccgccaga accgccaaca cacggctgac gccgacgact
    12541 ttcaccccct ccagtcccga cagctcggag gccatcaagt cgcccaccct ggccggatcg
    12601 aactccgcgc cgccgctatg attcagcgcc ggagccaccg cgatgcgcac ctcttcgggg
    12661 aggtacaacg ccggctcggc ctcttgttcc atgggttgcc gggctgccga acagcccgcc
    12721 ccaagcatcg caagaatgag gaagtatctg gatctatacc cccatgcgtt ctcgcggttc
    12781 cggcgagggt ctcgccgggg ctctcggacg accctatggc tttccacggc gatcatccct
    12841 gatcgagcac cctttccgta tggtgcagcg cggcccgacg gcgactgcgg ctcgcccgca
    12901 ccggtcccgg gtcagcttct gcggctcggt aacacaggcc cgagacattt ctagttcgta
    12961 gttcggctct tttcgggacc agcttaaacg cctggttccc aaaaaccctt tattcgcttc
    13021 tcctccattt gcttatcctc cccgcgggtt gccggcgggc acgacggcgc tgtgggcacc
    13081 cgcgggcgcg gcagcgctat catccgccgc cagcggggtt tggggcatcg cgaccgcctc
    13141 tgcaaggact gaccagatat tctcggaacc gctctgacgg ctggtgaagt agacgcgacc
    13201 gtccgccgcc cagcagggcc cgaagtgtgc cccctggccg tcggtcagtt gccggggtcc
    13261 ggttccgtcg gcccgcatga tccagatgtc gcctcgcgaa gcagggggca ggggtccggc
    13321 ggcgggatca gccgggaccg gctgtggcag agcagttcca tatgccaccc aatcgccatc
    13381 cgggctccag gtcggctgaa tgaaggccat ctgcgaactg gccgccaatt cgatcgggaa
    13441 tcgcggttcg ccgtcctgaa ggtcgattcg ccagatgctg aaccatctgc cgccgcgctg
    13501 gcgtgcgcgc tggtaaagaa tgctgtcgcc tttggggctc caccgcggga agaggcccac
    13561 gccgatcatt cgccgcgcat cggggcgatc aagttgaagg atccacaatt cccattgctc
    13621 ggcccggacg ttgaacatgc agtacactac ttgccggccg tcgggtgaga acgaggggtg
    13681 aatctcgtgc cagggtgatc gggtcagttg cgttgcctgg ccgttgtcgg taccgatgag
    13741 ccaaaggtcc cagttgccag aacgattgct cgcgaaaaca atgctcttgc cgtcggggct
    13801 gaaacagggc tgaacgtcgg gcgccggatc ggaagtgagc tgggtgacgg ccacaccgtc
    13861 gaccgtcttc atatagaggt cggcctgcgt ggaatgccgg gttgaggcga ataccagatt
    13921 ctttccatcc ggtgaaacgt cgacgtcgaa atccgctccc tcggcggtga aggagtgctg
    13981 cttcatcggg gcgttggccc tgggatggaa gggtaagcgg gccaactcgg ggagttcgcc
    14041 gaagagggtg atctgggcgt cctgggccgc gggcatggcc ggctcgggtg gcggaggagg
    14101 aagcggtcgt tcgctctcct ccttagcctt ctcgaagcag gtgcatccca cgaggcacgt
    14161 ggtgatgata gccgcacata ctgccccgcg acctcggcga accattctgg cccgtgtgtt
    14221 gattggcatg cccatgactg tcccgatcca gttcccatcg agaagagagt gatcgggctt
    14281 cttatcggtt cctcagcaac accgttcgac ttaaatccgc cgccatcctg gcaggcctgt
    14341 tccgataata tgctgcgtat gtatcgacgt ccggtcggtg ggctcttcag atgttttcgg
    14401 cgcggaaggc aaggattctc ggacctttca aaatcgcgca agaatcgccg gcaaacgggc
    14461 aaaagatgcg catggagacg gatcttgggg catcaggccg ccgacatgcc cgccagtcga
    14521 gagcgtatat tgaagtcacc aaaggatctc ccagcggcca cttggcgccg gggttccatc
    14581 cgtcttgcgg cgggtcgggg agccggggct ggatcgaagc aaggcttcag aacggccctg
    14641 ccagtactgc cgtgcggccc ggcggtctgg agcatccggt cgagcgactt tgtggccgcc
    14701 tgcccgtttc gtagaatgcg gctgtggaca cgcacacgct ggagaagatt gatttcgatc
    14761 gtgttcgtca gatcctggca gaacatgcga gctgcgcgct gggacgggca atggtctcga
    14821 aggtaagacc cgtcgtgcgg gaggaccttg ttcggcaatg gctccggcag atcgaggaga
    14881 tgatcgaggc gagcgccacg atcggactcc cgccgtttgg gggcgttcgg gatgttcggg
    14941 aaatcgtccg ttcggccatt cccccccacc tgccggagcc ggctgagttc gccaccgttg
    15001 ccgagacgct cgaggcgact caccggatcg tccgctgggg cgacaagctg agctcggcat
    15061 ccggcgaact gcggcgagtc tgcgacaaga tcggcgactt ccaggccatc gcggacgtca
    15121 tccatcgagt tattgacggc agcggcgagg ttcgcgacga cgccagtgcc aagctccggg
    15181 ccattcggac ggagattgcc aatgcccgca tcagcatcgg gcacgtattc gaccgcatcc
    15241 ttcgcgaccg gcatgtgacc cgctggctgc ggtacgccaa tgccactttt cacgaagatc
    15301 gacttgtcct gccgctggcg gccgagcacc gcgggcgggt accgggtatc gtgcaccgca
    15361 gctccgattc gggggccacg ctgtttgtcg agccggccga ggccgtcgaa ctgaacaacc
    15421 ggatcatcac cctcaagaac gacgagcaaa cagagatcag ccgcctgctc tggaacctga
    15481 cacaccagtt gcacctcaac agcgtggaaa tcctcaagac tctggatgcc ctggccatcc
    15541 tggacctgat cgcggccaag gcgcggtttg cccgatccta cgacctgcac attccgcaga
    15601 tctccaacga cggctcgctg cgacttcggc aggcaaggca tctcctgctg gtcgagatgc
    15661 aaaaacaggc ggcgggccgc ggcgaaaagc gcgaggtcgt accgatcgat attcgccttg
    15721 gggatgattt tgacatcctg atcatcaccg gaccgaacac cggcggcaag accgtggttc
    15781 tcaagacagt cgcactggcc tgcacgatgg cccaggcggg cctgcccata cccgcggccg
    15841 agggaagccg cgtgccgatc tatcacgata ttctggtgga catcggagac gaacagagcc
    15901 ttcagcagtc gctcagcact ttcagctccc atatctcgcg ggtgatggcc atgatcaaac
    15961 acgcacggcc gggtacgctg atgctcatcg atgagctcgg cgcgggcacc gacccggagg
    16021 agggcgccgc cctggggcga gccattgtgg ctgagctgct caaacgcaag tgtccggccc
    16081 tggtgaccac tcatctggga gtgctgaaga gcctggcgta tacggagccc cgcgcggaaa
    16141 acgcctgtgt ggacttcgac gcccagacgt tacggccgac gtaccgcctg ctgatcggcg
    16201 agccgggtaa cagcaacgcc atcaatatcg cctcacgact gggcctgccg ccgcacatcg
    16261 tcaaggcggc tcgggccttc ctggccgact cgcatcagca gctcagccgc gcgattgccg
    16321 gcacgttgca gtcgcgccgc ctggcggagc tggcacggca gcaatccgaa gccgctcgtg
    16381 tcgaagcgga aaagcagcag gcagccgccg aaaacgcccg tcgcgaactc gaagagcagc
    16441 aggcccgctt cgaacgttgg gtcgcaatga ttctttcgct ccagcccggc caccgcgtgc
    16501 tcgtccggcg cttcgaccgc gagggcacca tcgtccgcat gctcctgcac aagcagatgg
    16561 ccgtcgtctc ggtcggggcg atggagatgg aagttccgat caaggagctc ttgcctgtga
    16621 cggacgatca ttgatgatcg gtggcggtgc tcctctttcc ggctcccagc agattaagcg
    16681 tatcagagca tatgtgaagc tcgggctggc aggtcttcgc cgcctccggc ggctccgacc
    16741 gtgccctacc catgtgaaac gtgttcttaa gagccattgg ttcctggctg tcagcgagca
    16801 ttcggcaggg cggcggcgag gtcgccgggg cgaccgagac ctgccatatt gttgagctcg
    16861 ggcgggcagg tcttcgccgc ctccggcggc tccgaccgtg ccctacccat gtgaaacgtn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttcttaagag ccattggttc
    17041 ctggctgtca gtcagccgtt agcctcatac ccgtggcttg gtcgaagaca agcgctctcg
    17101 cagtcgcgtg gcggcggcga ccaagtgcga cagtttctcg aacgcctcgt cgatcgtcgg
    17161 cggctcgacg gcctcgggag cgaaaccgca gtcgggattc aaggcgatgc gattgggact
    17221 gaggatctcc gccccggcgg ccgcgaggga ttcgatctgc tccaccgttg gaatctgttc
    17281 gcctcgcacg tcgacgacgc ccattccaac ggacaggtgt gaaggcagga gcttgaggtc
    17341 gctgatgtcg cctgtgtcct tgtaggcgaa ctcgaggtta acgcggtcaa ccttcaaatc
    17401 cttgaaggcc ggcagaagcg gcttgtagtt gccggtcacg tcgctgcgcc gcttgtagac
    17461 gctgtggcag caatgcaagt gagtctcggc gcggaggttc tcgaccacgc ggttgatggc
    17521 cgcgatcgcc atggggagct ctttctccgg gttccatgcc cgccgcagac gctcgtcgtg
    17581 cgaactgatc ccggccatga gatcccggtc gcagaagtac accagcgcgg gatcgtcgat
    17641 ctgcacgatg tcgataccgg cctgcaccag cgcccgtgcc tcgcgagcca agatctcggt
    17701 caggtgttca agaaagtgct ccatggtcgg gtaggcctcg cggctgtgat cggcgtgcca
    17761 gtagcgcaag gcgatcagga acgggctggg cagggcgaac ttggtcaccc gctcggtgtg
    17821 accggccagg aaagccgcat cctcggcgaa gaccggctcc gtggcggcga tacggcggac
    17881 gacaacgtcg gtgtacttca cttcgccttg atgctcgtac cgacgacatt tctcgaaacc
    17941 gccgatccgt cccagcagct cgcgaatgta ctgttcgcgc cgccactcgc cgtcgctgac
    18001 cacgtccatg ccggcctctt cctgaagccg gatggcaaac agcacgaagt cgtccatgac
    18061 gcgggccagg cgccccggat ccatctgctt gcggcggtcc aggacgctga gcagggcctt
    18121 cggccgtggc agcgacccga tcggatgggt gaagaacggc ggcagcttgg cggtgcgatc
    18181 agtcttcaag cggatactct cctatcccgc aaggacaccc aacggctgag tccggctatg
    18241 cagtcggacg aggcggcgct attatagccg acaatggcga aggcggccga tttctgcaca
    18301 ttcaagaacg ggacatcaac acatgaacag tgttttcctt gtccgaagaa cgcgtttacg
    18361 ccacgcctta tttggcttga tgctcattgc cgtttgtgca ccggccgtgt ttggcaaggc
    18421 cggtccggca ttcaagctcg tcggcaagga ggtgttcatt cccggccggc cgggcgtgag
    18481 ggtggtggcc caggccttct atgttcgcag cgaaggccag gagatgatca gccggtactc
    18541 cgagcagacg aagagcgaca aggcggacat cgcgtatcag cggttctccc acgacaacgg
    18601 gcggacatgg tcaacgcccg cgatgctggt cacgaacgag cgagtatccg gcggcgcagt
    18661 ggggccgacg ccctcgtcgg cccattccgt ctccgcaccg gtgacaaacg aatccgctcc
    18721 cggcggcacg cgccggcggg ccatgaaacc cgggttcgtc gatccgacac gggacgtgct
    18781 cgtcacggtg tttcaggagg cgatcctgcc gacggataat ccgctcgagg gcatgaagta
    18841 ctggacgctg cactatgccc tgagccgcga cggcgggaag acgaactacc acgaggggcc
    18901 gattgtccac aaagggacgg agtattctca cgagcatcct ctgccgaacg tctgggtcgg
    18961 aaagaactcg gcgatgatcg gcgacaccgc ctgcgtgccg atcagaatcc ggacgggaga
    19021 aatcctccag ccgatccaga tcaccccgat cggccccgac ggcgaatact acaaccccgg
    19081 cggcgggtac acctatcacg attctgccgt gctgatcgcc cgatggaacg actcgggcat
    19141 tctcgactgg gagttgtcca agctggtgaa gggcgacgcg acccgttcga cccgcggcat
    19201 gctcgaaccg acgattatcg aggcgcctga cggccgcatt ttgatggtgc tccgcggcag
    19261 caatgaccgc aaaccggaac tgcccggcta ccgatggtat gcggtgtcca ccgaccacgg
    19321 ccgaagttgg tcgccaccca agccgtggac gtacgacaac ggcgagtcct ttcactcccc
    19381 gagttcgtgc agccagcttt tggggcacag cagcgggcgc atctgctgga tcggcaacat
    19441 cagcccgtca aacccgcgcg gcaattcgcc gcgctacccg ctggtcatcg gcgaagtcga
    19501 tcccgagtcg ctgctgctgc ggaaggattc ggttctggtg atcgacgatc ggctgccggg
    19561 tgagccggaa tggacggctc tcagtaactt ctatgcccgc gaggaccgca tcagccgcga
    19621 gatcgtcgtg cacctctccc cgataggcaa aggtcttgcg cagtcctcac ggccggttgt
    19681 ccaggcaacg caaccgacct ttgagtggac ggccgatgca tgggtgtacc ggattgcggt
    19741 gctgatggcc gagccaagaa aacgagaatg atgacgcgat gcagtcggcc atcgcgcccc
    19801 gatccatccg gcgcggctcc aaggttctgt tctactcgat gccctgctcg gccagccatc
    19861 gctcggcatc gatggccgcc ttgcagccca tgccggcggc ggtgatggcc tgtcggtaaa
    19921 cgctgtcgac gacgtcgccg gcggcaaaga cgccgtccac gctggtcgtc gtccggtagg
    19981 catccttgag cttgacgtag cctttctggt cgagttcgag ctgcccctcg aggaaagccg
    20041 ttgcgggggt gtgtccgatc gccacgaaca gcccgccaac cggcagatcg ctgacctcgc
    20101 cggtcttggt gtccttgagc cggacgccgg tgatcaactt gtcgccgagc acgtcaacgg
    20161 gaatcttgtt ccagagcact tcgatcttgg ggttatcgaa gacccgcttc tgcatgatct
    20221 tgctggcgcg gagctgatcg cggcgatgga tcatgaacac cttcgaggcg aatttggtca
    20281 ggtaggtggc ttcctcgacc gccgaatcgc cgccgccgac gacggcgagt tccttgttgc
    20341 ggaacaccgg cagggctccg tcgcagacgg cgcaggcgct gacgccgccg ccgctgcggg
    20401 ccagccgttg ttcgttgggc aggcccagcc agttggcggt ggcgccggtg gcgacgatca
    20461 ggctgtgcgt ttcgatcatc gtggcgtcct cgccccagac cttgaagggg cgtgccgaca
    20521 ggtcaacctt ggtgacgtcg tggtaggagt aggagtggcc gtcgtcgggg ttggagacat
    20581 cggggttctg gccgttgtcg gtctgaatcc gtgcgccgaa acgaacggcc tgctgttcca
    20641 tccgagccat gagttgcggc ccggtgatcc gttccggaaa gccggggaag ttttcgatct
    20701 cagtggtcag catgagctga ccgcccggca aaacggtcga gggagtctgc ttggggcggc
    20761 cagcgaagac caagggcttg aggttcgccc gggcggcgta aatcgcggcc gtccacgccg
    20821 caggcccgga accaatgatg atgatcttct caaccataat cacctcccaa cggccggacc
    20881 gcgctacacc gcaaagcctc ggtctgggcc ggtgtcttgc cttgccggtc cgatgacggg
    20941 catgaatcgc gtggcatact ctgcttcagg gcgggcgccg catcgggaat catggcctgc
    21001 ccgccgcaag gccgaccgtg ccgtccgaaa cggcgaccgg cgattgtagc gctgatcctg
    21061 tcgggcgtcc acactggcgt tcggcaggaa acgaccgtat agtgcggcaa gtgcctgagc
    21121 cgatacacga caggaagtcg gcgcgggcaa agtctgtttg cgtccgcgac ctgccgtcga
    21181 aagggcctct cacaggttcg cggccggcgg tccaactgac agcggcggta gccgagtcac
    21241 gtggcctctc gtggcggaca ggacaagatg ctgaccatgc gcatagcctt attcttcgcc
    21301 gacgggcggg cgtacttcct gggaatagcg ctcgtcatca tcgggctgct cgtgctggcg
    21361 gccggcaaga aacgcaaacc ttcaagacga attcgcatcg ttctggcggc tcgcgtcctt
    21421 ctgatcatgg gggtcttgtt tattcttctt tccgctacac ccgctcccat atggttctat
    21481 ggtctgtggt tcatcacgat cgtcgcgtgg ctcatccatc agtcggagcc catgctgaag
    21541 cgcgaagtcg ggattgctct gcggatcggc tcgctgctgt tctgtcttgc cgcggctggg
    21601 atggagattc cgcaccggct gctgccgaag ctcgaaccgc aaggacagga aaaagtgtac
    21661 gtcatcggcg actcgctcag cgcgggcatg gggaccgagg agatgacatg gcccaaggtg
    21721 cttgcggcgg gcgtgcccgt ggagatcgtg gacctgtcgg ccgccggggc gacggccggc
    21781 accgccatgg agcaagccaa gcgcgtgccg ccgggcccgg ccatcatcgt gatcgagatt
    21841 ggcggcaatg accttctcgg gggaacgccc gggcacgttt ttgaaaatga tcttcgccga
    21901 cttcttgaga cgctgagcgg cgattcccgc cggctggtca tgtttgaact acctcttcct
    21961 cctttcttta acaagtacgg acgcattcag cgtcggctgg cgtcagagtt cgacgtcgcc
    22021 ctgatcccca agcgcatcct gtccgacgtg atcggctcgg cacgcggcac gctcgacggc
    22081 ctgcacctgt ccgacgacgg ccaccggcag atcgccgaga cagtccgccg ggtcgtcaat
    22141 ctgcccgaac cgaggggcgt gccggcttcg tgaccgttcg gtcggcaagc gcgacgaact
    22201 ggaatcaccg catcgacgca cacactgacg gtcctcaagc acggccgggg gtttctgctc
    22261 gacaggaaga aggtcaaagt ccagcgatta gtcgcgacgg ccggaagggg gccgcaggtg
    22321 gacactgatt gcggcggaga attgcggatg tgcttgatgt tgggtttgtg tggcgcgggg
    22381 ggggc
//
LOCUS       DAOTPW010000072        22364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2388_length_22364_cov_4.811242, whole genome shotgun sequence.
ACCESSION   DAOTPW010000072 DAOTPW010000000
VERSION     DAOTPW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22364
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_22364_cov_4.811242"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="P5316_08460"
     CDS             complement(<1..514)
                     /locus_tag="P5316_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRR85027.1"
                     /translation="MCVAYLICRLADTVEDEAALTDEQRALLYDILLAAVDAPDEPAL
                     AEEFRRRWPAIPADEYGRLVEGTPLVLAAYATLPAELRGPIRTCVHDMIGGMRSMGVV
                     EYRNGVGFVCRDMADLERYCHHVAGTVGIMSTALFEERLRQGGFAADAEWREDGRRLG
                     LGLQMTNIIKD"
     gene            complement(939..3362)
                     /locus_tag="P5316_08465"
     CDS             complement(939..3362)
                     /locus_tag="P5316_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRR85028.1"
                     /translation="MLKNVKIGTKLVGGFVLVALLLAGIWGTGVWGLTKVGHDLRVVS
                     DQCLPSVDGLGTVAKSLESVRVAQRTLLSTHLSAEDWKRQIDNINKANEGYAASWKTY
                     ENLPKTAEEAELCKQFAEAEKAWRESSESFLKAATDLKAKDIGDPAVLLASIQQFTGD
                     HYKLVMNLQSLVYNNEAFEGGHDPAACNFGKWMASLKTANPELKKILADVQEHHNAFH
                     GCVAKIRDLVAKADKEGAAKILHGEMANAMKEVFGEFANMRAEAQKSVDFYNTMHDLA
                     MVECRAKQEAVLKPLSRLIELNSTRAEAQSAQGKANSRLAMVTSGVGALIGVGLTLAL
                     GFSLTRGITRPLKQAVGLAKGISEGDLTQRLSLNQEDEVGQLVTAMNGMAARLCEIMH
                     DMNEKGSMLSCAAIELSATSTRLASGAEETTAQSATVAAAAEEMATNMNSMAASAEEM
                     SNSVKAAASAVEEMTASIGEVARNAEQAATVAENAAVLAKASNESISNLGAAADEIGK
                     VIEVIQDIADQTNLLALNATIEAARAGEAGKGFAVVATEVKELAKQTAEATEDIRRRI
                     EGIQSSTVSTVESIGQISGVIAKVNEVSRTIASAMEEQSIATKEIASNVSQTAVASGN
                     VSAGVTQSAAASREITENIAGVDLAAKQTAEGAAHTHAAGAQLQKVAEELQRLVGQFN
                     IGQTEFSAAPIKAAHSEWKRKLAELLAGQASLDPSEVTDHHSCQFGKWYFGEGMQRFG
                     HLSSFKSIDAQHAEVHRVAKEIAHSYKEGHKNKAAEQFREFYALTAELFAKLDRFEEE
                     AKRGAAVSV"
     gene            complement(3803..6004)
                     /locus_tag="P5316_08470"
     CDS             complement(3803..6004)
                     /locus_tag="P5316_08470"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRR85029.1"
                     /translation="MKLRSKIVSLGVTGVFLTAAVLVVLVLVQKGRVRTQLVEELDKQ
                     ARNECAAVAKGVYQMLRAQQASLNLKLESDLKVAGRVLRQAGEITFAPEKVTWDAVNQ
                     LTKQPTKVELPKMMVGQNWLGQNFDSKQPAPVVDEVKSLVGGACTIFQRMNEAGDMLR
                     ACTNVTAADGKRAISTYIPAVEPDGKPNPVVTTVLQGKTYVGRAFVVNAWFHTAYEPI
                     FDANKNVIGALFVGVPQESVKEVRDSIMKTVVGKTGYVYILGGTGDQKGKYIVSLGGK
                     RDGENIWEAKDADGHLFIQSIINKALVLKDDNCDFERYPWKNEGDVTARYKIAAISYF
                     QPWDWVIGAGAYEDDFQDAIASMDRALMSMLYWTIGGAVLTFVLCGLLSGFSAHRIVT
                     PLRRAAAALQDIAQGEGDLTRRLEVHTSDEVGDVAKWFNTFVEKLQRIIGEIAVSART
                     LAGSSTELSATATQLASGAEETTAQSATVAAAAEEMATNMNNMAASTEEMSANVKTVA
                     AAIEEMTASIGEVARNAEQAATVASQAAQLAAVGNENISQLGTAADEIGKVIGVIQDI
                     AEQTNLLALNATIEAARAGDAGKGFAVVATEVKELAKQTAEATEDIRKRIEGIQSSTG
                     SAVQSINEISGVIAKVNEVSSTIASAVEEQSTTTKEIAQNIGQTASAAETVSQGVAQS
                     AAVSKEITKNIAGVDQAAKQTAQGAAQTQAAGNDLSKLAEQLQGLVGQFKV"
     gene            complement(6758..7591)
                     /gene="tsf"
                     /locus_tag="P5316_08475"
     CDS             complement(6758..7591)
                     /gene="tsf"
                     /locus_tag="P5316_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRR85030.1"
                     /translation="MAEITAAMVKDLRERSGLPMMDCKRALEEASGDATKALELLRKR
                     GAAAAEKKAGRAMGEGRMGMYVDPAKGVAALAELSCETAPVGRNPEFIALANKIAKHA
                     ALTGNADAATIGAEQFVDDPSQTVQDLLIDVLNRIRENMKIARVARVRGRAAGYVHHD
                     GKLGVLLVVEGSGGSEALLNDLCMHIAAMAPKALRREDLPAELVEKEKEIQRAQIIAS
                     GKPENMVDKILIGKMNRYFSEQVLLEQPFVKDDKKTVGKVLEEAGLKAVTFLRMQVGG
                     A"
     gene            complement(7748..8599)
                     /gene="rpsB"
                     /locus_tag="P5316_08480"
     CDS             complement(7748..8599)
                     /gene="rpsB"
                     /locus_tag="P5316_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRR85031.1"
                     /translation="MASEFVRKLIDSGIHFGHRTSAWNPKMRPYIFGTRNQIHIIDIR
                     ETIKGILCAKKFVANVVAKGQDVLFVGTKRQARYAVMDAAKRTGMHYVSERWLGGTLT
                     NYRTIRSRLARLEELEAIEAEGMAGKYSKKMIATLTRERRKIKRNLEGIRSMTKLPGV
                     VVVIDVRREYIACLEARKLKIPVIGLLDTDSDPDYVDIAIPGNDDAMRAIELVCEAMV
                     EAIEEGKRARPAEGAGEAAATRGTAGAKRSSRAAAPAEEAGESTAQEGDEAASGQPVT
                     DSAVAST"
     gene            8841..10061
                     /gene="lpxB"
                     /locus_tag="P5316_08485"
     CDS             8841..10061
                     /gene="lpxB"
                     /locus_tag="P5316_08485"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRR85032.1"
                     /translation="MTVPESDKDAKPSCSPPARPRIFISAAEPSGDRHAAGLVREIRR
                     LCPKAHLFGVAGPAMQAAGCLAIDDLTSKSAMLVGALRLLRHARRLMRQISRILAATP
                     VDLAIVVDSPALHLPMAKRLRKAGCPVLYYIAPQLWAWAPWRIRRLRRRVNRVAAILP
                     FEEGYFRQRGVDARYVGHPLVEQLADYNPDHTLLAAMRQAGSPVVTCLPGSRSHVIQE
                     VLPGQIEVARTIAEHHPRAVFLFAAADARASAIIREFLPTDNLNCRVEIGHLPEMLSA
                     SDLALCASGTATLEVACHGVPMIVMYNGSKWGYRLVGRHLITTPHLSLVNILAGRRIV
                     PEFMPYYDSTEPIAAEALDILANPARQEAIKTDLARIVNSLGTSRAAEQAARMAVEMI
                     GSEPAASYNNSDAG"
     gene            10136..10399
                     /locus_tag="P5316_08490"
     CDS             10136..10399
                     /locus_tag="P5316_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRR85033.1"
                     /translation="MPTYEYRAADGQKACDYCRERFEIRQSMSEEPLAACPRCGNPVE
                     RVISLCAVSTAQSVKSMLSDKNLKSKGFTKLVNEGGGRFRKTT"
     gene            10823..11866
                     /locus_tag="P5316_08495"
     CDS             10823..11866
                     /locus_tag="P5316_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRR85034.1"
                     /translation="MTLRLVVLGLLLIGCVLVLRPFISAILWAIVLCISSWPICSRLA
                     DLLRGRRGLAAAILTVCIILILLAPFVLIGLSLADDVRALGSGLREFLGLPPLEPPGW
                     VERLPLIGSHAADTWRDLASDSAQLTSRLKPFVEPVTSALLAGSVHFMKGVAELALSI
                     FIAFFLFRDGAVVAQRFVEATEHIVGAQTRELVDVAAKTVRGVVYGILGTALAQSVIA
                     GIGFLIAGIPGAGLLALLTFFLSVVPMGPPLVWVPAAIWLFWKGWLGWGIFMVCWGLL
                     VSSVDNVIKPMIISQGSRMPFVLVLLGVLGGAIAFGFIGVFLGPVLLVVGLRLLDHWN
                     KTRLAQRRMGKSG"
     gene            complement(12124..13626)
                     /locus_tag="P5316_08500"
     CDS             complement(12124..13626)
                     /locus_tag="P5316_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR85035.1"
                     /translation="MTDREQLELLIHAGHPLISVVTGEEESAVNLVREVAFDMARDLA
                     GLGLWQWSVASGLRDGLVDAGKRVTTTENAAVALAQLTQLPGRYICVMLDLGPHLSDA
                     KTLRYFREAVCHCRQTGSHMVLIDYADQLPSVVAMEAARFEVSLPSETELDCIIREVV
                     RRLHRREPIEVDLTRRQLQTIIRNLRGLTRRQAERIITEVVLEDHRLDASDINGILAA
                     KRRTLDSGRLLEYVEAPVDLSEIGGLRRLKHWLKLRQQAMSDEAAKFGITAPRGVLML
                     GVQGAGKSLCAKAIATAWQRPLMRMDVGALYDKYIGESERRLRDALKQAESMAPIILW
                     IDEIEKAFASAASRSTDGGLSQRMFGSLLTWMQEHRAPVFLVATANDIEALPPELLRK
                     GRFDEIFFVDLPEASTRREIFAIHLRKRGRDPEKFDLDKLAERSEGFSGAEIEQAVVS
                     GLHHAFAEGAALDTDRLLQALEQSPPLSVTMAESVQALRQWAKGRCMPAD"
     gene            14010..15065
                     /locus_tag="P5316_08505"
     CDS             14010..15065
                     /locus_tag="P5316_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR85036.1"
                     /translation="MMIHSHHDSRRQFLRQVGRAALGGMALGFLPRPLSAQSRPTQTS
                     RVALTTGESRGDNIHRALRLMEPQIRETLARKKRVLLKPNIVSTGRQLAATHADCLEA
                     VLDFLSAFHKDEFIIADSSASGSSHEGFSNYGYDRLARKHKVRFLDFDDEPSTIVYVS
                     DHRFHPQAVRMSDLLLNPETYIVSAAILKTHDRAVVTLSLKNVVVGAAKKDKTFRWGV
                     PDSGSNDKMIIHGGPANEAINLNLFQLARRLHPDLSVLDGFQGMEHNGPVGGTPVDHK
                     VAVVSTDWLAADRVATELMGFDFNKVGYLSFAAKAGLGEGDLSRIEILGEKVTDHVRH
                     YRPHDRVEEQYKWMTRS"
     gene            complement(15062..16039)
                     /locus_tag="P5316_08510"
     CDS             complement(15062..16039)
                     /locus_tag="P5316_08510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85037.1"
                     /translation="MSPAYRSEIERALRLRRRLLEDDSLEAVRILNGAADGVPGLVME
                     KLGPVLMAQLHEQRLAMGEDAARAIAEQLCRRLGATAVYRKWFVRERGRLDRRIAAAH
                     TNPEPWFGQRAADEYAVWEHGLQFLVRPYDGFAYGLFLDHRDNRKRVRELSAGRRVLN
                     LFAYTCGFSVAAAVGGATQVASVDAHKRYLEWGKRNFEINGVALGPHRFFCSDIFDFY
                     KRARRQGLRYDLIILDPPTFSRQRQPRRVFVLEEQLEALCTGAIELLERDGLLLLATN
                     DRGLSRQRLEQAVQKAAGSRRCEIIERPGLPIDFAGDPDYSKTIIGRVG"
     gene            16441..16947
                     /locus_tag="P5316_08515"
     CDS             16441..16947
                     /locus_tag="P5316_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRR85038.1"
                     /translation="MARVGPYNGILRQMVTKYKFGRNQHLDRPLARLIKSAIEGRGWN
                     REIDALVPVPTTWYNRLRYRFRPAAQLARATGRELHIPVLPLIRVIGKKYNQMDLPAS
                     LRPGNVRGVFKLHPRARPAGARLCIIDDVCTTGATINEIARVLKKAGAAAVYAAVVAK
                     TEADQPIA"
     gene            complement(17091..17804)
                     /gene="rsmG"
                     /locus_tag="P5316_08520"
     CDS             complement(17091..17804)
                     /gene="rsmG"
                     /locus_tag="P5316_08520"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRR85039.1"
                     /translation="MLRMEPSELMSGTPERQAFDDALMRACGAIGVSPSKEQRARMFE
                     HFRRVIETNRHFNLTRITSPADAAAKHYADSLVLLATAWVQPEQPTHVLDVGTGAGFP
                     AVPLAIMCPRWQITAVDGTGKKARFVAETATVLGLANLRAIHSRGEDLARKGGGPFDV
                     IVVRAVGKIAEVLHGLRALINPGTSLVFYKTDKMEPAELSDGICQAGRLGLLPAEPFG
                     IELPSPDGVMCRRLIRFGP"
     gene            17992..19284
                     /locus_tag="P5316_08525"
     CDS             17992..19284
                     /locus_tag="P5316_08525"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRR85040.1"
                     /translation="MPEIAPFRALRYDTKRFSTDVSKLVAPPYDVLEAEDKAELLARS
                     DKNIVAVDLPYVPPSKAGPDAVYAQAADLLNRWKSDGTLVRDDQPALYIYHQAYEYAG
                     RAYTRKKFFARMRIEAFGKGTVFPHEQTFGGPKEDRFKLMRATQCQLSPVFGLYSDPE
                     NVISKLLDPGSRPPDMTADMQGVDNRLWMLQDPAAIDAVCKAFSGRAVYIADGHHRYG
                     TALNYRDTLIEEEGELPNDHPARFVLVGLCAMEDPGCIILPTHRTVSGFGYLTVDDIL
                     DVLRTGLALQEVAVPPTDAETLLKPDSPCDVVLYVPRGERVFSGTFLHRDVLAQLAPD
                     RSEAWHRLDLAYLHRYLIEELITGRAMGGSEPTVRYFKVTQAAIEDARQTGGIAFITK
                     ACTMHELRAVSHAGDLMPQKSTFFYPKLATGLVINPLA"
     gene            19404..20591
                     /locus_tag="P5316_08530"
     CDS             19404..20591
                     /locus_tag="P5316_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRR85041.1"
                     /translation="MPQDTYSRRRFIETTLAASGFTQVAVRLSQAAQTAQTPPATGPA
                     AGPVYDDQQHTSDLFKNTPRRHAFRAHTLDEFRQWQSSFRQRLRELLGIAAMEREVPF
                     EHRAEQVERVAKDGYTQEKWYLWTEPGVPLPIWVLIPEKPAAGPHPLVLTPHGHNVPE
                     IYLGVADGDADRKSIAEGQRDIAVQAVREGYLCIHPTARGFGETMRPEDRKSRKVHSC
                     RTGLMHAILFGRTMIGYRVWDISRIIDWATRRFEIDSRRIAITGNSGGGTTSLFAPAC
                     DQRISVAVPSCYFCTFEASIGSIHHCECNYVPGLLREGEMYDVAGLIAPRWFRAIAGK
                     DDAIFPIAAVHEAFHRLRNIYRVAQAEDHCSLYVGDGPHRYYSDGAWPFIRNAFAQLS
                     KTG"
     gene            complement(20734..21921)
                     /locus_tag="P5316_08535"
     CDS             complement(20734..21921)
                     /locus_tag="P5316_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRR85042.1"
                     /translation="MRRFLIAGSLIVVLLGSVAIVRADTTSPGSQPAGQVVSKEALRQ
                     KAMPAIDRAIRYQLSKQTESGGWEIVPGVADPAVTAMVAQCLIQHPDYGPKHPVVEKA
                     MGFVLSFQQPDGGIYSTQIGYRNYTTSVVLMALSSMKSPAVSDYIAGARKYLKDNQWT
                     EGKCDTEGRPITPDHPWYGGAGYGQSRRPDLSNTQMMLEALHQSGLPPTDPAYQKAIQ
                     FVLRCQMSERSNDQPFAKGADDGGFIYTPANGGESKAGTIMVKGRPRLRSYGSMTYAG
                     FKSMLYANVDRDDPRVKAAWDWIRRNYTLTSNPNMPGEKSKEGLFYFYHVFAKALEAW
                     GEPVLIDSANVAHDWRADLVDQLVGAQRPDGSWVNEADRWHEDNPSLVTAYAVLAMQT
                     VIR"
BASE COUNT         4169 a         7720 c         6054 g         4421 t
ORIGIN      
        1 agtctttgat gatattggtc atctgcagcc ccagccccaa acggcgccca tcctcgcgcc
       61 attccgcatc cgctgcgaac cctccttgcc gtaggcgctc ctcgaagaga gcggtggaca
      121 tgatgccgac cgtccccgcg acgtggtggc aatacctctc gaggtcggcc atgtcccggc
      181 agacgaagcc caccccattg cggtattcca ctactcccat cgacctcatg ccgccgatca
      241 tgtcatgcac gcacgtgcga atcggaccgc gcagctccgc cggcaaagtc gcatatgccg
      301 ccaggacaag aggcgtaccc tccaccagcc ggccatattc atcggcaggg atggccggcc
      361 accgacgacg gaattcctcc gccagagccg gctcgtccgg agcgtcgacc gccgccaaca
      421 gaatgtcata caacaacgcc cgctgctcgt ccgtcagagc cgcttcgtcc tcgacggtgt
      481 ccgccagccg acagatcagg tacgcaacgc acatcgcccg atccagcggc tcaggacatt
      541 gcggaatgat cagggcaaac gaacgggaaa cgtcgggcag tatgcccgca caaaaacccc
      601 aacacgcgtt ttgtaggctg ttgtgagtca atcgtggacg ccaatcccag gtcgttaatc
      661 cgaatacgtt ggcgtaccgg accgcccgca ccaccgccgg ctgccgccgc cgcgtgcgcc
      721 gcacactgct tcgtagtgta gcgtcattcc aggcacgacg ccacaaccgg gaagggtgca
      781 tctcacaagg cgcggccatc gcagccaaga ggcgagtggc cctggcctgc gtctgctgca
      841 agaggatact gatggactca tacggaacct tgtgcgggac aggcgtcttg ccggtccaat
      901 ggaccgcctg caggccggcc ccgttccggc cgagccccct aaaccgaaac ggcggcccct
      961 cgcttggcct cttcttcaaa tcggtcaagc ttggcaaaca actcggccgt cagtgcgtaa
     1021 aactcgcgga actgttctgc cgccttgttc ttgtgtcctt ccttatagga atgcgcgatc
     1081 tccttggcca ctcgatggac ttccgcgtgt tgtgcgtcga tgctcttgaa cgaagaaaga
     1141 tgtccgaatc gctgcatccc ctcgccaaaa taccacttcc caaactggca actatgatga
     1201 tccgtgacct ccgacggatc gagcgacgct tgtccggcta gcaactcggc cagtttgcgt
     1261 ttccactcac tgtgggccgc cttgataggg gctgcgctga attcggtctg gccgatgttg
     1321 aattgtccga caagccgctg caactcttcg gccaccttct gaagttgcgc tcccgccgca
     1381 tgagtgtgcg ccgccccttc agcggtctgt ttcgccgcca gatccacgcc cgcgatgttc
     1441 tcggtaatct cacggctggc ggcagccgac tgcgtcacgc ctgcagacac attgcccgag
     1501 gctacggccg tctgagacac gtttgaggca atctcctttg tagcgatgct ctgctcctcc
     1561 atcgctgacg cgatcgtccg cgaaacctcg ttcaccttgg cgatcacgcc gctgatctgc
     1621 ccgatcgact cgactgtgct gacggtcgac gactggatgc cctcgatccg cctgcgaata
     1681 tcctccgtgg cttccgcggt ctgcttggcc agttccttga cctcggtggc gaccaccgca
     1741 aagcccttgc cggcttcgcc cgcccttgcc gcttcaatcg tcgcattaag agccagaagg
     1801 ttggtttggt ccgcgatgtc ttgaatgacc tcaatgacct tgccgatctc gtctgccgcg
     1861 gcccccagat tgctgatact ttcattgctg gctttcgcga gaaccgcggc attctcggcc
     1921 acggtggcgg cctgctcggc gttcctggcc acctcgccga tgctggcggt catctcctcc
     1981 accgcgcttg ccgccgcctt gacgctattg gacatctcct ccgcggaagc cgccatgctg
     2041 ttcatattgg tcgccatctc ctccgcggca gcggccaccg tcgccgactg cgccgtggtt
     2101 tcctccgctc cgctggccag ccgagtggag gtggcggaga gctcgatagc cgcgcaagac
     2161 aacatgcttc ccttctcgtt catgtcgtgc atgatctcac acaaccgagc cgccatgccg
     2221 ttcatcgccg tgaccaactg acccacctcg tcctcctgat tcaggctgag tcgctgagtc
     2281 agatcaccct ccgaaatgcc cttcgccaaa cctaccgcct gcttgagcgg gcgagtgatt
     2341 ccgcgagtca ggctgaagcc caaggccagc gtcaggccga cgccgatcag ggcgcccacc
     2401 cccgatgtaa ccatggccaa ccgcgaattc gctttgcctt gggcgctttg cgcctcggcc
     2461 cttgtgctgt tcaactcgat cagcctcgac agcggcttga gcacggcctc ctgcttggct
     2521 cggcattcaa ccatggcgag atcgtgcatc gtattgtaga aatcaacgct tttttgtgct
     2581 tcggcccgca tgtttgcgaa ctcgccgaaa acctccttca tcgcattcgc catctcgccg
     2641 tggagaatct tcgccgcccc ttccttatcg gccttggcca caagatctcg tatcttcgcc
     2701 acgcagccgt gaaaagcgtt gtgatgctcc tgcacgtcgg caagtatctt cttgagctcc
     2761 gggttcgcag tcttcagtga agccatccat ttgccgaaat tgcacgccgc cggatcgtgc
     2821 cctccctcga acgcctcgtt gttgtatacc aggctctgaa ggttcataac cagtttgtag
     2881 tggtctcccg tgaattgctg aatgctggca agcaaaacgg ctgggtcacc gatgtccttg
     2941 gctttgagat cggtagcggc ttttagaaag ctctcactcg attctctcca tgccttttca
     3001 gcctcggcaa actgcttgca cagctccgcc tcttccgcgg tcttcggcag gttttcatag
     3061 gtcttccagg acgccgcgta accctcatta gccttgttga tattgtcgat ctgcctcttc
     3121 caatcctccg cactcaggtg agtactcagc agcgtccgct gagcaacacg cacggactcg
     3181 aggctcttcg cgaccgtacc caggccgtca acgctcggca aacactgatc gctgactacg
     3241 cgcagatcgt gccccacctt cgtgagcccc caaacgcccg taccccagat gcctgcaagc
     3301 agaagagcaa caagcacaaa cccgccaacc agcttggttc cgatcttcac gttcttgagc
     3361 atcgcaagtc tccctatctg gtcgcccggc cgatctcagc attccgaaca catcgcggca
     3421 gcagccaacc cccacacgat cagccttgca cgaccgcaca cggcaaccca ggatgtgcag
     3481 tacaaccacg aagtgtgcct ttcgcctcag ggtttcccct gagacccatc cgtagttcga
     3541 ctgagacctt ctctgacttg agcaccctgt gatggcgggc ttggcaaatc cgtggtgctt
     3601 gcttgaaatg gtagagataa gaggcccgat aacgtacgct actgttgacc aacccgaatc
     3661 tcagactgtg tctcaccagt agctgccaac gatggcttgc cgtcctcagc aatgaaacag
     3721 ggccccccga tcactcggac ggcccctgca atatggtcag tctggaccaa acggcctgct
     3781 ccggccgatc gagcggatcc cgctagacct tgaactgccc caccaggccc tgcaactgtt
     3841 cggccaactt cgacaggtca ttgcctgcgg cctgagtctg cgccgcaccc tgcgccgtct
     3901 gcttggcggc ctggtccacg cccgcaatgt tcttggtgat ctccttgctc accgcggcag
     3961 actgagcgac tccttgcgac accgtctccg ccgcgctggc cgtctggccg atgttctggg
     4021 caatctcctt cgtcgtggtg ctctgctcct cgaccgccga ggcaatcgtg ctcgacacct
     4081 cgttgacctt ggcgatcacg ccgctgattt cattgatcga ctgcaccgca ctgccggtgg
     4141 acgactggat cccttcgatt cgcttgcgaa tgtcttccgt ggcttccgca gtctgcttgg
     4201 ccagttcctt gacctcggtg gccacgaccg caaaaccttt tccggcatcc ccagcgcggg
     4261 ccgcctcgat cgtcgcgttc agggccagca ggttcgtctg ctcggcaata tcctgaatga
     4321 ccccgatgac cttgccgatc tcgtcggcgg cggtacccaa ttggctgatg ttctcattgc
     4381 ccaccgcggc cagttgcgcc gcctggctgg ccaccgtcgc ggcctgctcc gcgttcctgg
     4441 ccacttcgcc gatgctcgcg gtcatctcct cgatggctgc cgcaaccgtc ttgacgttgg
     4501 ccgacatctc ctcggtggac gcggccatgt tgttcatgtt ggtcgccatc tcctcggcgg
     4561 cggcggccac cgtcgccgac tgcgccgtcg tttcttccgc accgctggcc aactgggtgg
     4621 ccgtggccga cagctccgtc gatgagccgg ccagcgtcct ggcgctgacc gcaatctcgc
     4681 cgatgatccg ctgcaacttc tccacgaacg tgttgaacca cttggccacg tctccaacct
     4741 cgtcgctcgt gtgaacctcc agacgacggg tcagatcgcc ttcgccttgg gcaatgtcct
     4801 gcaatgccgc cgctgcccgc cgcaacggag tgacgatccg gtgagccgag aagcccgaca
     4861 gcagcccgca cagaacgaac gtcaacaccg ccccgccgat cgtccagtac agcatggaca
     4921 tcagggcccg gtccatactc gcaatggcat cctgaaagtc gtcttcataa gcgcctgccc
     4981 caatgaccca gtcccagggc tggaagtaac tgatggccgc gattttgtac cgggccgtaa
     5041 catccccctc gttcttccag ggatatcgct cgaaatcgca gttgtcgtcc ttcagcacca
     5101 gcgccttgtt gataatcgac tggatgaaca aatgcccgtc cgcatccttg gcctcccaga
     5161 tgttctcacc gtctctcttg ccgcccagag aaacgatata cttgcccttt tgatcgcccg
     5221 tgccgccaag gatgtagaca tagccggtct tgccgacaac ggtcttcatg atgctgtcgc
     5281 ggacctcctt gacgctttcc tgtggaacac cgacaaacag ggcgcctatc acgttcttgt
     5341 tcgcatcgaa gatcggttca taagccgtat ggaaccaggc attgaccaca aacgcccgcc
     5401 cgacatatgt cttgccttgc agcaccgtgg taaccaccgg attgggcttg ccgtcaggct
     5461 caaccgccgg aatgtacgtg ctgatcgccc gcttgccatc tgccgcagtc acgttggtgc
     5521 aggcgcgcag catgtcgccg gcctcgttca tccgctgaaa tatcgtgcac gccccaccca
     5581 ccagcgactt gacttcgtcg acgacgggcg cgggctgctt ggagtcaaag ttctgtccca
     5641 gccagttctg cccaaccatc attttgggta gttcgacctt cgtcggctgc ttcgtcaact
     5701 gattgaccgc gtcccaggta accttctccg gggcaaacgt gatctcgcct gcctgcctta
     5761 acactctgcc ggccaccttg aggtcgcttt caagcttcag attcaggctc gcttgttggg
     5821 cccgcaacat ctgatacacg cccttggcga cggcggcaca ctcgttgcga gcctgcttat
     5881 ccaattcctc caccaactga gttctcaccc ggcctttctg caccagcacg agaaccacca
     5941 gcacggcggc cgttaagaaa acacccgtga cacccaacga tacgatcttt gaccttagtt
     6001 tcatctcacg tgccctttct caagcatgcc cagcatccag ccagaacgct ggctacaggt
     6061 gatcacagtc tggaaccgca ctagcggccg gcacgagccg ttttgttcat ctgcctcaga
     6121 actctacgcc cagagatcaa tgcggcttgc gcgcattctc tcgaatcacc gtgcgattcg
     6181 gctgcggacc gcaaaagctg atccgcctca gacgcttacg atctgacaac cggagccgca
     6241 gaccgcctcg aagcctcaga acttacttcc aagcgttcgc accgccccgc aagccggact
     6301 gttcgcccgt atggcactga gccttttccc gatgaacgga cgaaccccgg gaccgctgat
     6361 atatgaatcg cctccgggtc gaacttcttt agcaactcag ggaaatcccc gatgctgtgg
     6421 ctcacctcca cctcgccaag cctgctggag acctgttgcg ccacctcatc tcagaataga
     6481 tcggcctctt ttatcggacc cacctcgcac gacggctgca gcaaaaccct gacgcacaac
     6541 cacgacagat ccctatatat gggtgccttt gcgagagtag actgttcaca agaagaaagc
     6601 tgcccggcaa ttcccggtgg cccttcgccg aaaaccgctg ggtccttggt tgcagaatgg
     6661 gatggaacgc ggcccgttca tgcgcggagt gcgcttctat tcctcgttcc ttgactgccg
     6721 gcgccgctat acgcagtgct caggacgacc gccccggtca cgcaccgcct acctgcatgc
     6781 ggagaaaagt gaccgcctta agaccggcct cttcaaggac cttgccgaca gtcttcttgt
     6841 catctttcac gaagggctgc tcgagcagaa cctgctcgct gaaatagcgg ttcatcttcc
     6901 cgatgagaat cttgtcgacc atgttctccg gcttccctga agcaatgatc tgggcccgct
     6961 ggatctcctt ttccttttca accaactcgg ccggcaggtc ctcgcgtcgc aacgccttgg
     7021 gagccattgc cgcgatgtgc atgcagaggt cgttgagcag ggcttcactg cctccggagc
     7081 cttcaacaac cagcagaaca cccagcttgc cgtcgtgatg cacatagccg gcggcacgcc
     7141 cccggaccct cgccacccgg gcaatcttca tgttctcccg aattcggttc agaacatcga
     7201 tcagcagatc ctgtacggtc tggctcggat catcgacgaa ttgctcggct ccgatggtcg
     7261 ctgcatccgc attgcccgtt agggccgcgt gtttcgcaat cttgttggcc agcgcgatga
     7321 actccgggtt cctgcccacc ggtgccgtct cacagctcag ttcagccaag gccgcgaccc
     7381 ccttggccgg gtcgacgtac atgcccatgc gcccctcgcc catggcacgg cctgccttct
     7441 tctccgccgc ggccgccccc cgcttacgaa gcagttccag cgccttcgtg gcgtcgccac
     7501 tggcctcctc cagcgctctc ttgcagtcca tcatcggcaa accgctgcgc tcgcgcagat
     7561 ctttgaccat tgctgcggta atctcagcca tgtgcttgct tgctccttgc gtgctacaca
     7621 cacccatacc cgacccaggt ccccagtcac ctgtgccgga aaacccaagt acacctggac
     7681 catcatgaac gacatcagaa acggcgcagg catggttcgc ccggccgccg cgcgtggatc
     7741 tcttatccta tgtcgaggcc acggccgaat ccgtcaccgg ctggccgctc gcggcttcgt
     7801 cgccctcttg cgcggtagac tcgccggcct cctcggccgg cgccgctgcg cggctcgatc
     7861 gtttcgcacc cgcagtcccc cgggttgcgg cagcctcgcc ggcaccctcc gcgggccgtg
     7921 cccgtttacc ctcctcaatc gcctctacca tcgcctcgca aaccagctcg atcgctcgca
     7981 tcgcgtcgtc gttacccggt atcgcgatat ccacgtagtc gggatccgag tctgtatcaa
     8041 gcaagccaat cacgggaatc ttgagtttgc gagcttcaag acaggcgata tactcgcgcc
     8101 gaacatcgat caccaccaca acgcccggca gcttcgtcat gctccggatg ccttccaggt
     8161 tccgcttgat cttccgtcgc tcccgcgtga gcgtcgcaat catcttcttg ctgtacttcc
     8221 ccgccatccc ctccgcttca atcgcctcca attcctccag ccgagccagc cgcgaacgaa
     8281 tcgtccggta gttggtcagc gtcccaccca gccatcgctc cgacacataa tgcattccgg
     8341 tgcgcttggc ggcatccatc acggcatagc gggcctgacg cttagtcccc acgaacagaa
     8401 catcctgccc cttggcaaca acattcgcga cgaacttctt ggcgcacaaa atgcccttga
     8461 tcgtctcgcg aatatcaatg atgtggatct gattgcgcgt cccgaagatg tatggacgca
     8521 ttttgggatt ccaggcactc gtccgatgac cgaaatggat ccctgaatcg atgagcttac
     8581 gaacgaactc tgacgccaaa cgacacctcc gcttgcgacc gacctgctga ctcgatgaag
     8641 cgtcgcgcaa cttcaccggg cgcgcggacc tcccgcgctc catacccaca cccgccccac
     8701 tgcgaggcag gctcacccct ttcgccgaaa agtcacagcc aggggcgata caggaagcga
     8761 tctatattac ccgcagacat gcagtcccgc cagcccccct tccctcgggt ttgacccgcc
     8821 ccccgaccgc tgatataccg atgaccgtgc ccgagtccga caaagatgcc aaaccatcgt
     8881 gctccccgcc ggccagaccc agaatcttca tctcggcggc tgaaccgagc ggcgatcgcc
     8941 atgcggccgg tctggtccgg gaaattcgcc gtctttgccc caaggcccac cttttcggcg
     9001 tcgctggacc ggcaatgcag gccgcaggct gcctggctat tgacgatttg acgtcaaaat
     9061 cagccatgtt ggtcggtgcc ctccgactgc tgcgccatgc gcggcggctc atgcgccaaa
     9121 tctcccgaat acttgcggcc accccggtcg atctggctat cgtcgtggac tctccagcgc
     9181 tgcatcttcc gatggctaaa agacttagaa aggccggatg tcccgtcctg tactatatcg
     9241 ccccacaact gtgggcttgg gctccctggc ggattcgccg cctccgacgc cgtgtgaacc
     9301 gggtcgcagc catcctgccc tttgaagaag gctacttccg acagcgaggc gtcgacgcca
     9361 ggtatgtggg ccatccgttg gtcgagcagc ttgccgacta caacccggat catacgctgc
     9421 ttgcggccat gcgccaggcc ggttcgcccg tcgtcacgtg cctgccgggg tcgcggtccc
     9481 atgtgatcca ggaggtgctc ccaggccaaa tcgaggtggc ccgaaccatc gctgagcacc
     9541 atccccgcgc cgtattcctc tttgcggccg ctgatgcccg tgcctcggcc atcattcgtg
     9601 agttcctgcc caccgacaac ctcaactgcc gcgtcgagat cggtcatctg cccgaaatgc
     9661 tgtccgcctc cgacctggcc ctctgtgctt caggcacagc tactctcgaa gtggcctgcc
     9721 acggggtccc gatgatcgtg atgtacaacg gatccaagtg gggctaccgc ctggtcgggc
     9781 gacacctcat cactacgcct catctttccc tggtgaatat cctcgccggc cgtcgaatcg
     9841 tgcccgaatt catgccctac tatgattcca ccgagcccat cgccgccgaa gccctcgata
     9901 tcctcgccaa cccggctcga caggaagcga tcaaaaccga tctggccagg atcgtgaatt
     9961 ccctgggcac ctcgcgggca gccgagcaag ccgcccgcat ggccgtggag atgatcggct
    10021 ctgaaccggc tgcgtcgtat aataatagtg acgcgggctg aggtggctcg taagaactcg
    10081 cgccggaaga ggccgggcga ggcatcccgc gagatggttt tctgtgggcg accgcatgcc
    10141 gacttacgag tatcgcgcag cagacggaca aaaggcttgc gactactgcc gcgagcgatt
    10201 cgaaattcgc caatccatga gcgaggagcc gttagctgcc tgcccaaggt gcggcaaccc
    10261 ggttgaacgg gtcatctcgt tgtgtgcggt ctcgaccgcc caatccgtca agtccatgct
    10321 cagcgacaag aacctcaaga gcaagggctt taccaagttg gtcaatgaag gcggcggccg
    10381 tttccgaaaa accacatgag cggcaccgcg cggctccgca gtgcttgagg cgcggaccct
    10441 ttcgtggaga ctcaaggtcc gctacatcgt ctttcggggc tgtcggcctc gttctcattg
    10501 cacccgcttc tctggctctc ccaagcttga tcgcccgctc agcatctcgt ttgccctgct
    10561 cggcgcgacc ctgtgcggga tcttcctgac ccccgtgttc tactacgttc tgcaatgggc
    10621 ggcggagtgc ggaaggccga aacaagtccc ggcggccgcc ctaacagggt ccggcaaccc
    10681 cagccactcc aaatgatggc cacgcacgag gggaaaccgg tcgacacacg aacaagaatc
    10741 cttattatct ggggtcatga accaacagga gcgaaaccaa cggaaagatg atctgtcggc
    10801 accgaaggaa ccggccacca cattgacctt aaggctggtg gtcctcggcc tgctgctcat
    10861 cggctgcgtc ctggtcttgc ggccgttcat ctccgcgatc ctgtgggcca tcgtgttgtg
    10921 catctcgagt tggccgattt gctcccgact tgcggacctt cttcgcggtc gtcgcggcct
    10981 ggccgccgcg attctgacgg tgtgcatcat cctcatcctt ttggccccgt tcgttttgat
    11041 cgggcttagc ctggccgacg acgtgcgggc tttgggctct ggccttcgcg aattcctggg
    11101 gttaccgccc ctggagcctc ccgggtgggt tgagcggctt cctctgatcg gttcgcatgc
    11161 ggccgacaca tggcgagatc tcgcttcgga cagcgcccaa cttacctcgc gactcaagcc
    11221 tttcgttgag ccggtcactt cggcgctgct cgccggcagc gtgcatttca tgaaaggcgt
    11281 cgccgagctg gccctcagta tcttcatcgc cttcttcctt ttccgtgatg gtgcggtcgt
    11341 cgctcagcga ttcgtcgaag ccaccgaaca catcgtcggc gcccagactc gtgaactagt
    11401 cgacgtcgct gcaaagacgg ttcgaggggt cgtctacggc atcctcggca ctgccctggc
    11461 ccagagcgtc atcgcgggta tcggttttct catcgcgggc attcccggtg ccgggctgct
    11521 cgcgttgctg acattctttc tttcggtggt gccgatgggt cccccactcg tctgggtccc
    11581 ggccgcgatc tggcttttct ggaaagggtg gctgggctgg ggcatcttta tggtgtgctg
    11641 gggactgctg gtcagcagcg tcgacaacgt catcaagccg atgatcatca gtcagggcag
    11701 ccgcatgccc ttcgtgttgg tcttattggg tgtcctgggc ggagcgatcg ccttcggctt
    11761 catcggcgtc tttctcggcc ccgtcctcct ggttgtcggc ttgcggcttc tcgatcactg
    11821 gaataagacg cgactggcac agaggcggat gggaaagtca ggatgacggg ggaagcggtc
    11881 gggcatcagt tgtcagtcgt cagtgctcag tgcggagcct tcggcagggc tgaagactga
    11941 ccattgatag cttggtttgg ccattcgggc ttcagactcc cttggtcatt ggtgcttggg
    12001 tattggtcat ttcctcgaaa ccggggtgtt tgacaaatcc cgccgcatgc tcatggctca
    12061 tcctcgccag gttgcgttcc ggtccattca tccgccttca actcgggaaa ccgtcagtac
    12121 ccgtcaatcc gccggcatgc agcgcccttt ggcccactga cgcagagcct ggacgctttc
    12181 ggccatcgtc accgaaagcg gcggcgattg ctccaaggcc tgcaagagcc ggtctgtgtc
    12241 caaggccgcc ccttctgcaa acgcatgatg caacccggaa acgacggcct gctcgatctc
    12301 cgcgccactg aatccttccg accgctcggc cagcttgtcc aaatcgaact tttccggatc
    12361 gcggccgcgt tttcgcagat gaatggcaaa gatctcccgc ctggtcgacg cctccggcag
    12421 atcaacgaag aagatctcgt caaatcgccc ctttctcaag agctccggcg gcagggcctc
    12481 gatgtcgttg gccgtggcga ccaggaacac cggcgctcgg tgctcctgca tccaggtcag
    12541 cagcgagccg aacatccgct gcgacaaccc gccatccgtg cttcgcgagg ccgccgaggc
    12601 aaacgccttc tcgatttcgt caatccacag gatgatcggg gccatgcttt cggcctgctt
    12661 cagggcgtcg cggagccgcc gctccgactc gccgatgtac ttgtcataca aggcaccgac
    12721 atccatccgc atgaggggcc gctgccaggc cgtcgcaatg gccttggcgc acaggctctt
    12781 gccggcaccc tgcacgccaa gcatcagaac gccccgcggg gcagtgatgc cgaacttggc
    12841 agcctcatcc gacattgctt gctgcctgag cttcaaccaa tgcttgagac gccgcaggcc
    12901 gccgatctcg gacaggtcca ccggggcctc cacgtactcc agcagtctcc cgctgtccag
    12961 ggtgcgccgc ttggcggcca gaatgccgtt gatgtccgaa gcatccaggc gatggtcctc
    13021 caggacgacc tcggtgatga ttcgctcggc ctgccgtcgg gtcaggccgc gaaggttgcg
    13081 gatgatcgtc tggagctggc ggcgcgtcag gtccacttcg atcggctccc gacggtgaag
    13141 acgacgaaca acctctcgga tgatgcaatc cagctccgtt tcactgggca gcgaaacctc
    13201 gaaacgcgcc gcctccatcg ctacaaccga aggcagttgg tcggcgtagt caatcagcac
    13261 catgtgactg ccggtctgac ggcaatgaca gactgcttcg cggaagtacc gcagcgtctt
    13321 cgcgtcggat aggtgcggac caagatcaag catcacgcag atgtagcgtc caggaagctg
    13381 agtgagctgc gcaagggcga ccgccgcgtt ctccgtggta gtcacacgct tgcccgcgtc
    13441 gaccaggcca tcccgcaatc ccgaggcgac cgaccactgc cacaagccca gccccgctag
    13501 gtcccgggcc atgtcgaagg ccacttcacg aaccaggttg acagcagatt cctcctcgcc
    13561 ggtcaccacc gaaatcaatg ggtgccccgc gtggattagc agctccaatt gttcgcggtc
    13621 ggtcatgaac agatgccctc attgccgggc ccggcaggga tcgccgccca ccatcttcaa
    13681 gcataccaca ccgcaccgac aagtaaacgc ccgcgtcttg cggaagggcc tacgaatgga
    13741 tgaaaggtag ggcatggtcg gagccaccga aggtggcgga gacctgccgg tcctggatgt
    13801 ggaacgcggc cagtctctac cgctgtgccg acctcgatgc tgccctccgt gcggcaccgt
    13861 caacgcgtgt gccaccgttg cagcgatgca cacccgaatc accgcctcgc catgccgcga
    13921 caaccgtcct tgacagcacg ccccgcacga gattacatga acaagtcgca aacgttgact
    13981 ctgttcctca ccggccggag agcctcgcta tgatgatcca ttcacatcac gacagccgtc
    14041 gccaattcct tcgccaggtg ggccgcgccg cgcttggcgg catggccctc ggcttcctgc
    14101 cgcgccccct ctcagcacag tctcgcccga cccaaacaag ccgcgtcgcc ctgacaacgg
    14161 gcgaaagccg aggcgacaac atccaccgtg ccctcaggct gatggagccc cagatccgcg
    14221 agacgctcgc ccgcaagaag cgcgtcctgc tcaaaccaaa catcgtatcg accggtagac
    14281 aactggccgc aacccacgcg gactgtctcg aggccgttct cgatttcctc tccgcttttc
    14341 acaaggatga gttcatcatc gctgactcct ccgcctcggg atcctcgcat gaaggcttct
    14401 cgaactacgg ctacgaccgg ctggcacgca agcacaaggt ccgcttcctg gacttcgacg
    14461 atgagccttc gacgatcgtc tacgtgtccg atcatcgttt tcatccccag gccgtccgca
    14521 tgtccgatct gcttctgaat cccgagacct acatcgtctc ggcggcaatc ctcaagaccc
    14581 atgaccgggc cgtcgtcacc ttgtcactca aaaacgtcgt cgtcggggcc gccaagaaag
    14641 acaagacctt tcggtggggt gtgcccgaca gcggttcgaa cgacaagatg attatccacg
    14701 gcgggccggc caacgaagcc atcaacctta acctcttcca acttgcccgg cgcttgcacc
    14761 ccgacctgtc ggtcctcgac ggcttccagg gcatggaaca caacggcccg gtcggcggca
    14821 cgcccgtcga tcataaagtg gccgtcgtca gcactgactg gttggcggcc gaccgcgtcg
    14881 ctaccgaact gatggggttc gactttaaca aagtcggcta tctctcattc gccgccaagg
    14941 ctggtctcgg cgagggcgat ctgtcgcgca tagagatcct cggcgaaaaa gtgaccgacc
    15001 atgtccggca ctatcgccct cacgaccgcg tcgaagagca gtacaagtgg atgacccgct
    15061 cctaacccac ccggccaatg atcgtcttcg agtagtccgg atcgcccgca aaatcgatcg
    15121 gaagtccggg ccgttcaata atctcgcatc tccggctccc ggccgccttc tgaactgcct
    15181 gctccagtcg ctgtctgctg agcccgcgat cgttggtggc taacagcagc aggccgtccc
    15241 gctccaacag ttcaatcgcc cccgtacaca gggcttcaag ctgctcttcg agcacgaaca
    15301 cacggcgtgg ctgtcgctgc cgggagaatg taggcggatc aaggatgatc agatcatatc
    15361 gcagcccctg ccggcgggcc cgcttataga agtcaaaaat gtcgctgcaa aagaatcgat
    15421 gaggcccaag agccaccccg ttgatctcga agtttctctt gccccactcg aggtatcgct
    15481 tgtgagcgtc cacgctcgcc acctgggtcg cccctccgac ggccgcagcc accgaaaagc
    15541 cgcatgtgta cgcgaacaga ttgagaacac gccgacccgc tgaaagctcg cgcacccgct
    15601 tccggttgtc gcgatggtcc agaaataacc cataggcaaa gccgtcataa ggccggacta
    15661 ggaactgcag cccatgctcc caaacggcat actcatcggc agcccgctgc ccgaaccacg
    15721 gctccgggtt cgtgtgagcc gccgcaattc tccgatcaag acgcccccgc tcgcgcacga
    15781 accacttgcg atacacagcc gtcgccccca gccgccgaca taattgctcg gcgatcgctc
    15841 gtgccgcgtc ctcgcccatg gcaagccgct gctcgtgcaa ctgggccatc agcaccggcc
    15901 ccagcttctc catgaccagt ccgggcacgc catccgctgc accattgagg atccgtaccg
    15961 cttccaggga atcgtcctcc agcaaccgcc gtcgcagtct gagcgctcgc tcgatctcgc
    16021 ttctgtacgc cgggctcatc aagacctcca ctgcccctgc gtcccgccag gacacaaccc
    16081 ctccggatgc tacagacgcg gcaatcggat tgtccagcaa gtccgtaccg gcctggttgc
    16141 gcgacgccgc agcatcttgc gtcattcccc cgatggcatg aaaataccgc cgccatgtcc
    16201 tcgtccacac gagtttgggc atcgctgctc gacttgctta tgccgcgggc ttgtccagcc
    16261 tgcaaccgaa ccctctcggc ctcggatgac gtctggtgcc ccgcctgtgc catgacccta
    16321 ttggagtccg tgacgccccc ggatcgttac tgctcccgct gcgggcaatc agtcggaccc
    16381 tatctcttgg atgcttccgg ttgcgccgcc tgccgtgata cccgcttgtt cgtggaccgc
    16441 atggctcgtg tcggacccta caatggaatc ctccgtcaga tggtgaccaa gtacaagttc
    16501 ggccgtaatc agcacctcga tcggcctttg gccaggctca tcaagtcggc catcgaaggc
    16561 cgcggctgga accgggagat cgatgccctc gtgcccgtac ccacgacgtg gtacaatcgg
    16621 cttcggtatc gcttccgccc tgcggcacag ctcgcacgag ccacgggccg cgaactgcat
    16681 atcccggtcc ttcccctcat ccgcgtgatc ggaaagaaat acaaccagat ggatctgccc
    16741 gcctcgctgc gacctggaaa cgttcgcggc gtgttcaagc ttcatccgcg ggcacgaccg
    16801 gccggtgctc gactctgtat catcgacgac gtctgcacaa ccggagctac catcaatgag
    16861 atcgcccgcg tcctcaagaa ggccggcgcg gcggctgtct atgcggcggt ggtggcgaaa
    16921 accgaggcgg atcaaccgat cgcgtagggg aacgcaggag aagaatacgg cgctcacgca
    16981 cgatgcgcaa atcggcgctg cctgcgttgg tgtcaaagcc gtgccgaccg gatcgaactc
    17041 cgcaagagac tgtacagggc cccgcccgct gacgcagggc aagcacggcc tcatggcccg
    17101 aacctgatca accgccggca catcacgccg tcgggcgagg gtagctcgat cccgaacggc
    17161 tcggccggca acaggcccag ccgcccggcc tggcaaattc catcggataa ctcagcaggc
    17221 tccatcttgt cggttttgta gaagaccaac gacgtcccgg gattgatgag cgctcgcagt
    17281 ccatgaagca cctcggcaat cttgcccacc gcgcgaacca cgatcacgtc gaatggtccg
    17341 cccccctttc gggccagatc ttcgccccga ctgtgaatcg cccgcaaatt ggccagtccc
    17401 agaaccgtcg ccgtctctgc gacaaaacgg gccttcttac ctgtcccgtc caccgctgta
    17461 atctgccacc gaggacacat gatcgccaac ggcacggccg ggaaaccggc acccgtgccc
    17521 acatccagaa cgtgagtcgg ctgctcgggt tgaacccaag cggtcgcgag caataccagc
    17581 gaatcggcgt aatgcttggc cgccgcatcg gccggcgacg tgatccgtgt caggttgaaa
    17641 tgccggttcg tctcgatcac ccgccggaaa tgctcaaaca tccgcgcacg ctgctccttg
    17701 ctcggcgaca cgccaatggc cccacaagcc cgcatcagcg catcatcaaa ggcctgccgt
    17761 tcgggcgtcc cgctcatcag ctcgcttggc tccatacgca gcatgataac cgcggacccg
    17821 gccgggtact tagcgccgga cttgatgaac aaccctccgg cccataagct ttccgcctga
    17881 cccgatgaga ccaaggtaag ccacgggctt cctgttcaag caagccacgg gccttcctga
    17941 aatcggactt ctcataatca tgccccaggt ggcatacgaa aggatgaacc cgtgccggag
    18001 atcgccccgt ttcgcgccct gcgttacgac accaagcgct tttccaccga tgtctcgaaa
    18061 ctcgtcgccc ctccgtatga cgtgctcgag gccgaagaca aagctgagtt gctcgcccgg
    18121 tccgacaaga acatcgtcgc cgtcgacctg ccctacgtgc cgcccagtaa ggccggtccc
    18181 gacgccgtgt acgcccaggc ggccgacctg ctcaaccgct ggaagagtga cggcacgctc
    18241 gtccgggacg accaacccgc gctttacatc taccatcagg catacgaata cgccggacgc
    18301 gcctacacgc gcaagaagtt cttcgcccga atgcgcatcg aagccttcgg taaaggcacc
    18361 gtcttcccgc acgaacagac cttcggcggc cccaaggagg accgcttcaa gcttatgcgg
    18421 gcgacccaat gccagttgtc gccggtcttc ggcctctaca gcgacccgga aaacgtcatc
    18481 agcaaactgc tggaccccgg cagccgtccc ccggacatga ccgccgacat gcagggagtc
    18541 gacaatcgcc tttggatgct gcaagacccc gcagcgatcg acgcggtctg caaggcattc
    18601 tccggccggg cagtctacat cgccgatggc catcaccgtt acggcaccgc cctgaactat
    18661 cgcgacaccc tcatcgagga agagggcgag ctgcccaacg accatcccgc ccgcttcgtt
    18721 cttgtcggac tttgcgccat ggaggatccc ggttgcatca tccttccaac tcatcgcacc
    18781 gtcagcggct tcggctacct gacggtcgat gacatcctcg atgtgctgcg gaccggcctg
    18841 gcgctccagg aggttgcagt acctccaacc gacgccgaga ccctgctcaa gcccgattcc
    18901 ccctgcgatg tggtcctcta tgtcccccgc ggcgagcggg ttttcagcgg aacattctta
    18961 catcgtgatg tcctggcgca gttggcgccc gatcggagcg aggcttggca cagactggat
    19021 ctggcttacc tgcatcgcta tttgattgag gaactgatca ccggccgggc catgggtggc
    19081 tccgagccga cggtccgtta ctttaaggtg acacaggctg ctatcgaaga cgcacgccag
    19141 accggcggga tcgccttcat taccaaggcc tgcaccatgc atgaactgcg agccgtcagc
    19201 cacgccggcg acctgatgcc tcagaaaagc acgttcttct atcccaagtt ggccacgggg
    19261 ttggtcatca atcccctggc gtagacgagg ggccgtgttc tgccgatgca gcgtgcgcct
    19321 gatcgcgtcg gcaagtgggg acggagtcgt ccgcagtcac agtcggcagg gagtttgctt
    19381 ccattctccg aaaggagccg atcatgccac aagatacata cagccgacgc agattcattg
    19441 agacgaccct ggccgcgagc gggtttacgc aagtagcagt ccgactctct caggcggctc
    19501 aaaccgccca gacaccgccc gcaaccggcc ccgccgcagg tccggtttac gacgaccaac
    19561 agcacaccag cgatctcttt aaaaacaccc cccgccgaca cgcatttcga gcccacacgc
    19621 tcgacgagtt tcgccaatgg cagtcttcgt tcagacaacg actccgcgaa ctcctcggca
    19681 tcgccgccat ggaacgcgaa gtgcccttcg aacaccgcgc cgaacaagtc gagcgcgtcg
    19741 caaaggacgg ctatacccag gaaaaatggt acctgtggac cgagcccggc gtcccgctgc
    19801 ccatctgggt gctgattccg gaaaagcccg ccgccggacc gcaccctctg gtcctgacac
    19861 cccacggcca caacgtgccg gagatctacc tgggtgttgc cgacggcgac gccgaccgca
    19921 aatccattgc cgaaggtcag cgggacatcg ccgttcaggc cgtgcgcgag gggtatctct
    19981 gcatccatcc gaccgcgcgg ggcttcggcg agaccatgcg ccccgaggac cgcaagagtc
    20041 gcaaggtcca ctcctgccgg accggcctga tgcacgccat cctcttcggc cggaccatga
    20101 tcggctatcg cgtgtgggat atctcacgga tcatcgattg ggcgacgcgg cggttcgaga
    20161 tcgactcccg ccgaatcgcc atcaccggta acagcggcgg cggcacgacg agccttttcg
    20221 cacccgcctg tgaccaacgg atcagcgtcg cggtccccag ttgttacttc tgcaccttcg
    20281 aggcctccat cggctcgatt caccactgcg agtgcaacta cgtgcccgga ctcctgcgcg
    20341 aaggcgaaat gtacgacgtc gccggtctga tagcgccccg gtggttcaga gccatcgccg
    20401 gcaaggatga cgccatcttc cccatcgccg cggtccacga ggcctttcat cgcctcagaa
    20461 atatctatcg cgtggcccaa gccgaggatc actgcagcct ctacgtcggc gacggccccc
    20521 atcgatacta cagcgacggc gcatggccct tcatccgaaa cgccttcgcg cagctcagca
    20581 agacgggttg agaatcagca ggtagggcag gtcgaagccg cctgcggcca cgaagacctg
    20641 ccgtgtgtgg gcgggtggcc cacccgtttg ccgcagcgca tggatggggc ctcgatgatg
    20701 aattgccaag aagtgccagg agctaagagg gctctatcgg atcaccgtct gcatcgccaa
    20761 aaccgcatac gccgtcacca acgacggatt gtcttcgtgc cagcggtcgg cctcgttgac
    20821 ccaactccca tcgggcctct gcgcgccgac caactggtcg actaggtcgg ctcgccagtc
    20881 gtgagccacg ttggcggaat caatcagcac cggctcgccc cacgcctcaa gcgccttggc
    20941 aaaaacatgg tagaaataga atagtccctc cttcgacttc tcccccggca tattgggatt
    21001 gctcgtcagg gtgtaattgc gccggatcca atcccaggcg gcctttaccc tcgggtcgtc
    21061 gcgatcaacg ttcgcataga gcatgctctt gaatcccgcg taggtcattg atccgtagga
    21121 tcgcagccgc ggccggcctt tgaccatgat cgtacccgcc ttgctttccc caccgttggc
    21181 cggagtgtat ataaagcccc cgtcgtcagc tcccttggca aagggctgat cgttgctccg
    21241 ctcggacatc tggcagcgga gcacgaactg gatggccttc tgatacgctg ggtccgtcgg
    21301 cggcaagccg ctctggtgca gggcctcaag catcatctga gtgttcgaca ggtccggacg
    21361 ccggctctgg ccgtagcccg ccccaccgta ccagggatga tccggcgtga tcggcctgcc
    21421 ctcagtatcg cacttcccct cggtccactg attgtctttg agatacttcc tggcccccgc
    21481 tatgtaatcg ctcacggccg gactcttcat cgaagacaac gccatcagca ccacgctggt
    21541 ggtgtaattg cgatagccga tctgcgtgct gtagattccc ccgtccggtt gctggaaaga
    21601 aagcacaaat cccatcgctt tctcgaccac cgggtgcttc ggcccgtaat ccggatgctg
    21661 gatcaggcac tgggccacca ttgcggtcac cgccggatcc gccacgcccg gcacgatctc
    21721 ccagcccccg ctctccgtct gtttgctcaa ctggtacctg atggcccggt caatggccgg
    21781 catggccttc tgccgcaacg cctccttgga aaccacctgc cccgcaggct gactgcccgg
    21841 cgaggtcgta tccgcccgca caatcgccac cgaccccaaa aggacaacga tcaagctgcc
    21901 tgcaatcaaa aaccgcctca tgacgacaca acctccccga gccaatcccc caaaccaggg
    21961 acctccgctc acggccccac ccggtcattc tacccccgaa ggcgtcaaca aaccaaagca
    22021 caaccaacgc cgccgcagca gaggaatgag acgtcgaccg ccacaggtga cgcggtcaac
    22081 gccatgcaag gcccttgacg aacacttctc agaaccttac ggccggatat ctgtccggct
    22141 tacgcgcatg tttctggatc gcagaacgtt tgggctgtgg cccgcaaaaa ggcagtccgc
    22201 cttagtccct tccgtcgcaa ctggtgtgcg ttttgcgcgc atgtttctgg atcgcagtgc
    22261 gccgggtgcc atgcccacgg ctttgcgtgg gcatgcgcct ccaaatgaac cgctcagccc
    22321 ggaatccttt ccgggccgca tcccccgccc gctcagcccg gaat
//
LOCUS       DAOTPW010000073        22256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2400_length_22256_cov_6.003423, whole genome shotgun sequence.
ACCESSION   DAOTPW010000073 DAOTPW010000000
VERSION     DAOTPW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22256
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2400_length_22256_cov_6.003423"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..935)
                     /locus_tag="P5316_08540"
     CDS             complement(402..935)
                     /locus_tag="P5316_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85043.1"
                     /translation="MSHDPIQAERLSQRQADEGSAPRRCLDCGYIIDHLPEPRCPECG
                     KDFDPNKPETYWIRPTNAKTNGINYVLKGTLAFLAAPAFIVISRLPSMGWLEPARGPL
                     CGFVAVLGIGFGFAYLSGTVATARGAIPPWALKNHGIRLICGPGLTVLEHVCWAIAGF
                     CFAVGLLMFAGLFWSLL"
     gene            complement(964..2787)
                     /locus_tag="P5316_08545"
     CDS             complement(964..2787)
                     /locus_tag="P5316_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR85044.1"
                     /translation="MRLLSLFQISLARKCQLLFGLAVLLIIAAALFVPGYYMGSFVDQ
                     MHVQRAQQLAAVAAARIDPAAADWRQQQKALDQWWEVNEKVIPFLKECRPRVIKLLPA
                     DSEEVAAQQRQEFARLIGNLSAQVRPVVEKLKRRWDPPAAWQVARFAWESLPEHKRDH
                     CLKAIRRAAADLGRAFAIGNQWYPPLDEFQKNCVERMLNEGSSGEGGPMRSPDGPTVY
                     RYIQAVRGSPSSPSTGHPLIGIIDISLSAPITDELRFLTRAILVLAGLLAGFLAILVF
                     YLISQRLILAPVRELTALAGRIAGGDLQARASLQTGDEYEKLSDAFNDMLVRLEQARA
                     ELETANRSLDAKLGELAETNVALFESNRIKSEFLANVSHELRTPLTSIIGFADLLRDS
                     MLSGGQIDVARAARFADNILVSGRGLLDLINDLLDLAKIEARKIELHRTVFSLRDVCE
                     ALCDFVRPNIEKKSLVMTTDIDENLPMMTSDAGKLRQILFNLLSNAIKYTPEGGSVRL
                     EVRVLAGGRWVRLAVIDNGPGIAPEDQGRIFEKFHQLDSSVTREHSGSGLGLAISKEL
                     CTMLGGTIRVESELGQGSRFIVELPVECPESAERPPIPLRA"
     gene            2999..4516
                     /locus_tag="P5316_08550"
     CDS             2999..4516
                     /locus_tag="P5316_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85045.1"
                     /translation="MRKRRVAAAWGTWLMVAGVVAATVAHALYYQSDLYRRGLEADLV
                     GFFGLPTEVGSISPHSFTARQLDDVRVWLPEKRDLVFRCPRVIWDKSGAPGGHEASLD
                     IHEPVWLLGSEAWRSHDYTRLLQAGLKHNFQAVKLGIVRLHGARLEWQWPGCNLHADG
                     VDGKLVFDVQGSGHADLVCHQFNGHVTSAPIHISARIDPEADDLLPEVVLVVPELPLT
                     SLGLDQILQSPVTQGSFCGTITLMQSRGGDRIVLSGRAEKVRLDEWTKRIPGGAVSGL
                     LDLTIEKLVVQDRQLRELRFRGEAAEIAVDPLLARFGWPQLGGIARLRVLDGQVAEGI
                     VRNLHVLGSWDGGSLDGLCQRLFGKTGLEGRLRARINSLVIENERIAGGDIELSGEPP
                     AGQTGTIKKALLTEAFEKVFGLWLPSQLLPDRVQYTQMGVRILIDHEEVRVLSGLGPA
                     GPALITARILGQDLPLGGQLDLHLSIDQLLDQVKGQLNLLPPDLRRRLLTATRPT"
     gene            4768..6174
                     /gene="gndA"
                     /locus_tag="P5316_08555"
     CDS             4768..6174
                     /gene="gndA"
                     /locus_tag="P5316_08555"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HRR85046.1"
                     /translation="MAKQQVGLIGLAVMGENLALNIESRGYSMAVFNRTYKKTEDFMN
                     GRAKGKKFIGAKTLEEFVDALEKPRKIILMVKAGQAVDDFINMLVPLCDKGDLFIDGG
                     NSHFPDTIRRNKDLSAKGFLFIGTGVSGGEEGALKGPSIMPGGQKEAYDMIAPVLTAI
                     AAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLICEAYDIMRKALGLEAKALHEV
                     FKKWNAGDLNSYLIEITTDIFKRIDDETGKPLVDMVLDTAGQKGTGKWTSASALDLGI
                     AAPTIAEAVFARCISAIKKERVEASKQLSGPSPGYKGDKEAFIQAIHDALYASKICSY
                     AQGFQLMRAASEEYKWSLKYGEIAMIWRGGCIIRAHFLNRIKEAYDRNPNLANLLLDP
                     YFKGIIDKSQANWRKVVSTAADLGIPVPAFSSALAYYDSYRSEKLPANLLQAQRDYFG
                     AHTYERIDKPGVFHSDWT"
     gene            complement(6280..7821)
                     /locus_tag="P5316_08560"
     CDS             complement(6280..7821)
                     /locus_tag="P5316_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85047.1"
                     /translation="MFGGALSWAHYKIRGGGRNIALTVAAYVILAATVILLALRSSPS
                     DRAWSLDRCTDVLIVLQAVTLVLVGGNAVRNSIRKDLTSGMIQSHRLMPVSGPQAVTG
                     YIVGSTSQAIAIAVANLIIGLFTTAGGSGSQLDWLMDNAALGAFAVLAWSAIALLSFL
                     TPAATGLLVMFIIFAIATGGLIVSLLPALTVLLTPALSAAFSGTGPSIDFKVSLPAQA
                     ALAMFCFVGAARKFHREDVLPFTPLMGLALVAFWATLTWLGIVSMDRFFGSAGPRSLG
                     PGDPLPPAVQIVSSTLLGVLVAIVPVMASARLAAAHHQRRLLRDVAIGSRPLSPNIMV
                     LAVTVILCTIPLALIAREMRVYHSPWTSTMTRNLGYTAVTIALNLITISYLMRILYRV
                     TGKSAVMVFLFVLCTWIGPLALDFIRFGLSGMQPLDDIVTGLSACGPVGTMILIWGQF
                     QGSVELGLSIQACCAAIFALLFHGTQPRKVVPEARAAGLALGISHPNAAPAAPPEEQA
                     DSPSP"
     gene            complement(7836..8795)
                     /locus_tag="P5316_08565"
     CDS             complement(7836..8795)
                     /locus_tag="P5316_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR85048.1"
                     /translation="MVATADNEDVLARVDRVRVQFGSLVAVRDVSLTLRPGYLVGLIG
                     PNGAGKTTLLRAIAGLQPLSTGRIEVLAERVEPGWVGYKHHIGFTPDTPWLFDQLTVR
                     EFLRFIAKGYHISPETVDERIDFWLDQVWLTEKQNQKIKELSRGMRQRVGIARTLLPN
                     PSVVLLDEPAAGLDPAGRAQFRRLLCSLRHQGKALIVSSHILADLNEYCTHIAIMAHG
                     SIVQHGTVEQVANAGNGETGRYTVILVRPVPDLKDRLSQIEGIGAVEVDGDRFIVEFS
                     RHREDAAGLLLQLIRLKLPVASFAPNTPDLEEAYLRTGIRQVD"
     gene            complement(8889..9110)
                     /locus_tag="P5316_08570"
     CDS             complement(8889..9110)
                     /locus_tag="P5316_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85049.1"
                     /translation="MNHNMTLLASGWIDAALVVAILLAATLFLAMLVVRFLRGQITCA
                     CEKRRKGCATTQVRLSPGQLAKDPSKKNA"
     gene            complement(9132..11321)
                     /gene="feoB"
                     /locus_tag="P5316_08575"
     CDS             complement(9132..11321)
                     /gene="feoB"
                     /locus_tag="P5316_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRR85050.1"
                     /translation="MSIHEHPFHESTGKPRSRVEGRIGEFAARRGETKHATITAAIAG
                     NPNSGKTSIFNSLTGMNQSVANYPGVTVERKTGHACHGGREITLVDLPGTYSLTAYSL
                     DELIARDFVIESKPDVVVNVVSAASLERNLYLTVQLMEMQRPLVIALNMSDLAKRQGI
                     RIDTAKMSVLLGAPVIETVGNRGIGLGALLEATADTATDPAANKLRPVSYGHEVETEV
                     EKVAEVIAREERLIGRYPARWLAVKLIEGDKRVLQKVRQLAGNPAAIESASAAAVAAI
                     ESHFGESAEIIIAERRYGFAAGIVRECVHSTAESRQNITDKIDSVVCHRVFGPLLVLA
                     VVYSLFVAIFKVADEWKWLFGRSPKGWIEWLFEDAAPRLVAGLEEPMPLLHSLLHDGV
                     IGGVGGVLAFVPLIATLFAFVAILEDTGYVARVAFVLDRLLRVFGLQGKSMLAMIVGG
                     GLGAGGCAVPAVMATRTLREEKDRLVTMLVVPLMNCGAKIPVYLMLIAAFFASHKPQI
                     MFSLWAASWVIALGAAWVLRKTVFHGEQTPFVMELPAYHVPTLRGVLTHTGERVWQYI
                     RKAGTVILAVNLLIWAAMSFPRLNTADGADQLRHSIAGRIGSAIEPLTRYAGFDARDN
                     VALIGGFAAKEVVVGTMGIIHANDDSSARRAEGLSAKLAADPSWSPTRALALMVFVMI
                     YSPCSATLMVIRRESGKWRWALFAMAYTTVLAFIVATAVYQIGRLIG"
     gene            complement(11333..11632)
                     /locus_tag="P5316_08580"
     CDS             complement(11333..11632)
                     /locus_tag="P5316_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRR85051.1"
                     /translation="MPSLLSELHDGQTGRIVRVGGSGAIRQRLLDMGITRGSTVLLKR
                     RAPLGDPLQITVKGSDLAIRVKEARYIQVEILDDGDRSAAADGKAHGTPTPGSQR"
     gene            12288..15014
                     /locus_tag="P5316_08585"
     CDS             12288..15014
                     /locus_tag="P5316_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRR85052.1"
                     /translation="MHKILLRAVLYRLFLWTAIWAGCLAGGLRVARADEYRAFWVDGW
                     GAGFLNQSQVDTLLGVVGDPNSKGRIREANCNMVIVQVRRRADVCYPSGMGEPYFSGL
                     SPSNFNALQAMISAAHDTTGGKKRVEVHCWSVVFKTANGLVYSLHSDPANPDNYWPTR
                     LGSTTGSENSDGAFDPGHPKCLEYLVNVHMDLVTRFDIDGIHYDYIRFEGNTEGYNPT
                     SVARYNERYGLSGDPSSSSEQFKQWRRDQVTAFVRQMYARIQAVKPHVKQSGSFVTWN
                     PSPTSSTRAAFMATRPYYDVYSDWDSWMEEGIVDMAVPMTYYDWASLPNDYTRWMNFE
                     KDRKFNRHMIVGPGIYLNSLSNALYELQQTRWASPAGNYAQGFSGYSYRVPYSGGTWA
                     GFSPSLVADVTPTWDDVPTMPWKTNPTTGHICGTVTQVPDGAWADHATVSITGPVSRS
                     MHVDGTGFYAFIDLPPGSYSITASKAGYANAVSSADVAIGQVTGNMYVRDLVLGGNPL
                     PVISSVQATNVTDSGATITWLTDLASSSQVEFGLTTSYGNLTPPDSTPVTSHSVTLSG
                     LVANTLYHYRVISTNANGASISSDYSFKTAASTTELVIDNTDQECTRTGSWNTGSIAD
                     VPKVGTNYLYTPGTGNGLSVTATCRWTPTIVRAGLYDVYVFYQMGSNRNSAAPYTVVY
                     DGGQVVSIQNQYSSTPNQGGWFLVGENLPFAAGTGGYVECANNSTDTRYISADAAKLV
                     FKSGIELPPAKAAGPNPSHQATGIWTHTGLSWTAGEGATSHRVYFGTSSPGSFQIETA
                     DAVFDPGALLAATTYFWRIDEVNASGTTTGDVWEFTTRQTVAADLDRDGDVDAADGDL
                     FESCVSGPGVSLDEGCADRDLDGDGDADQSDFGVFQRCLSGTDVPVDPGCGG"
     gene            15278..16411
                     /locus_tag="P5316_08590"
     CDS             15278..16411
                     /locus_tag="P5316_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR85053.1"
                     /translation="MLNKVLVLSASAGAGHLRAADAILKAIHELRAAKEARHIDSLDY
                     TNKAFRSLYSKAYIELVNAAPDVLGWLYDALDKPWKNERRRLALDKLNTRPFVKMLEE
                     YQPDIAVCTHFLPAEIISWLKAKKRLRCRQAIVVTDLDVHAMWLCHHYEHYFVAMDET
                     REHLITLGIPAENITVSGIPIDPVFARPKDKVETRIKHDLDPDKTTILISAGGFGVGP
                     IEHLMISLLKLRHTVQIVAVCGRSEELRKRLERLVVRQPVDSHIRVKVVGFTTEMDEY
                     MAAADLVVGKPGGLTTSEALARGLVYVIVNPIPGQEERNADHLLEEGVAIRCNNLPVL
                     GYKVDRLLSEPGRLAAMKANVQRIARPRAAYDIVNKLRELAVG"
     gene            complement(16589..16822)
                     /locus_tag="P5316_08595"
     CDS             complement(16589..16822)
                     /locus_tag="P5316_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HRR85054.1"
                     /translation="MAKRRKRSDRDIEKAYPVKQFAAKLRRMAACLEKDLPFRIQIAG
                     ERVRIPPHATISVEHEREGKTEEVEIQFKWRNP"
     gene            complement(16901..19093)
                     /locus_tag="P5316_08600"
     CDS             complement(16901..19093)
                     /locus_tag="P5316_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRR85055.1"
                     /translation="MGNDSHLFVIATLFGSLAPCVRAAWDTRSLNDLTGPWQLMVDDY
                     CVAEKANVARTYHPFEKHPGNPVLTYDRPWEGTNVYLYGTVLPNESGRGFRMWYHALP
                     GDDDTYRLLYATSDDGIKWHKPNLGIVEYKGSKDNNIFIRRGKRDHILSVLHTPWDKD
                     PNRRYKMINFDGDPAKNGYVCAFSPDGLHWTDASDKPVFSKGGDVSQFLWDSHTRQYL
                     GFVKNNVHVSGMRRRAVARTASKNILSWSDPQLVLAPDTFDDRWAKGIQRTHFYGLSA
                     FAYESMYLGFLWVFRATDDEGYFDGPVHLELVTSRDGVRWFREEGERPPILELGKDGT
                     WDDGMHYTPQHPLVVDGKLWLYYGGFDCTHAAPAPWHGSIGLATLRKDGFASLDAGEK
                     TGIILTKKLAGTAGPLRLNYQCRAGGSVRVEVLEAEGKHLSGYTRDDCEPLTGDSLDQ
                     TVTWNNRNELPVADAPIRLRFVMENASLYSFTAGPKLQIIDEAAGPTLAVLYTFEDGW
                     TDSLREDGKQQAVQHGEIRPKTDPELAAFGSAGCPLGSPWCPLNTIEIKGTKNLGTQF
                     TLALMAKNKESKPARLISSTNDQGPVKTTELVFDCDPKGQVIPGLRLICKGIAVESKP
                     LRFADGKYHHLAVTYDDGLITFYLDGAPAGGGRVPAGEPISMTRNLFVGEDADHGREE
                     QLRGSVDDILVLGQLLSAHEIRTISQKGAEAVLQSRDRTSKKQPLSAE"
     gene            complement(19506..20612)
                     /locus_tag="P5316_08605"
     CDS             complement(19506..20612)
                     /locus_tag="P5316_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85056.1"
                     /translation="MRHLLTACAIALSTFSQQIARCDDTGRAILAQSIADAVFRGPPD
                     SYDVTYCETVVVPPQKIEVIREMVTKVYDATDNRAVNREGTPQRETELEEEVQRIHKA
                     QQDMKPHRRVARIRISGEQTRIDQTVLESGEPFKVTDVFAETRISARGKLIWYDHARR
                     SALVQKQAKPWRKTKQDLDDWCATPASLRIFGAALLGKPGNPATRELILDREKLSVFV
                     STGDQNVEVNVRPLEDPSLSSLEFRSKDNPAKRTVFIVDHVDYSRVQEVAVYDGEHLI
                     SRTRYEDRDKANVPKKVRIEAWDQDGKLLTSRDINIISAAVNCNIPSEVFEWKPPAGY
                     IQNDRTGEKLQVSGEEDWVSWQLTKPRRGQPADQ"
     gene            complement(20681..21229)
                     /locus_tag="P5316_08610"
     CDS             complement(20681..21229)
                     /locus_tag="P5316_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85057.1"
                     /translation="MNRDALIVALTLVSLLSADRRSEGGNSYDCQKPTTQPAVWDDEC
                     PECIEQGNEPACIGGVYRTYQWTSCEPAGLGEVGYTGCDEQGAVIGTFEMCEANWNVD
                     WVVFCAATTTACVVTCGSCIAAPTPVNCYACAECLAQQAAFGGLCSGCDLLEGCEPNG
                     IQQDVMRNVFKEQYGEACQGEP"
BASE COUNT         4383 a         6660 c         6862 g         4351 t
ORIGIN      
        1 gccaggatcg caacgacgac caggacttcg atcaacgtga aggcttgatg ggtcaggcac
       61 cggcgcataa tcggtagctc cccgcacgcg gcggactatc ggaaccacaa ttgatgcaac
      121 gggtgcttcc ccgggggcaa cggcgaatta taccgcgcgc cagctctctt gccaaggtat
      181 ctccaaaggt tccccgggag cgagggtaca tccctgaaac ggtggcacag gtatcggcgg
      241 ccgtcacggt aggccggtgt ccaggtcgcc accgcgaccg agacctgccg agaacggaca
      301 ggatcaccgg caggtcccat ccgctgcgcg gatttcgacc atgtcctgcc ttcggaaacg
      361 ccaccgaaga cgggatacat tccgagaacg atcgtcggtc gtcacaggag gctccagaac
      421 aggccggcaa acatcaggag gccgactgca aaacagaaac cggcgattgc ccagcagacg
      481 tgctcaagta ccgtcaggcc gggcccacag atcaggcgga ttccatggtt cttcagagcc
      541 cagggaggga tggcgcctcg tgccgtcgcg acagttcccg agaggtaggc aaacccgaac
      601 ccaatgccga gaacggcaac gaacccacac aggggaccgc gagccggttc caaccatccc
      661 attgacggca gtctgctgat cacaatgaac gccggcgccg ccaggaaggc cagcgtccct
      721 ttgagaacgt agttgatgcc gtttgttttg gcatttgtgg ggcgaatcca gtacgtctcg
      781 ggcttgttcg ggtcaaaatc cttcccgcac tccgggcatc tcggctcggg caggtggtcg
      841 atgatgtacc cgcaatcgag gcatcgcctc ggtgcagaac cttcgtcggc ttgcctttgc
      901 gataagcgtt cggcttgaat ggggtcatgg ctcacgagaa gggcaccatg cggccgggtc
      961 gcgtcaggca cgaagcggga tcggcgggcg ttcggccgac tcggggcatt caacggggag
     1021 ctcgacgatg aaccgcgagc cttggccgag ttcgctttcc acgcgaatgg taccgcccag
     1081 cattgtgcaa agctccttgc tgatcgccag ccccagcccg cttccgctgt gctcgcgggt
     1141 caccgacgag tccaactgat ggaatttctc gaagatcctg ccctggtctt ccggtgcgat
     1201 gcccggcccg ttgtcgatca ctgccaaccg cacccaccgg ccaccggcga gcacgcggac
     1261 ttcaaggcgt accgatcctc cttccggcgt gtacttgatg gcgttgctca acaggttaaa
     1321 caggatctgc cgcagtttgc cggcgtcaga ggtcatcatc ggcaggttct cgtcaatgtc
     1381 cgtcgtcatg accagggact tcttctcgat gttcgggcgc acgaaatcgc acagcgcctc
     1441 gcagacgtcg cgaagcgaaa agacggtgcg atgcagctcg attttgcggg cctcgatctt
     1501 ggccaagtcc agcaggtcgt tgatcaggtc cagcagtccg cggccgctga cgaggatgtt
     1561 gtcggcaaac cgggcggccc gtgcgacgtc gatctgccca ccgctgagca tggaatcccg
     1621 caacaggtcc gcaaagccga taatcgaggt cagcggagta cgaagctcgt ggctgacgtt
     1681 ggcgagaaac tcgctcttga tgcggtttga ctcgaagaga gccacgttgg tttcggccag
     1741 ttcgccgagc tttgcgtcga gggaacggtt ggccgtttcg agttcggctc gggcctgctc
     1801 caggcgaacg agcatgtcgt tgaaggcgtc ggaaagcttt tcgtactcat cgccggtctg
     1861 gaggctggcc ctggcctgaa gatcgccgcc ggcgatccgg cccgccagtg cggttagttc
     1921 gcgaacggga gccagaatca gccgttgcga gatcaggtag aaaaccagga tcgccaggaa
     1981 cccggccagc agcccggcca gcacgaggat ggcgcgagta aggaatcgta actcatccgt
     2041 gatcggcgcg gacagactga tgtcaatgat gccgatgagc gggtggccgg ttgacggcga
     2101 agagggcgat cccctgacgg cctgaatgta tcggtagacc gtcggtccgt ccggtgagcg
     2161 catcggccca ccctcgccgc tggagccttc gttgagcatt cgttcgacgc agttcttctg
     2221 gaattcgtcc agcggcggat accactggtt accgatggcg aatgcgcgtc ccaagtcggc
     2281 ggccgcccgg cgaatcgctt tcaggcaatg atctcgcttg tgctccggca gggattccca
     2341 tgcgaatcga gccacttgcc aagcggcggg gggatcccat cgccgcttca gcttctccac
     2401 gaccggacgc acctgcgcag acaggttgcc gatgagccgt gcgaattcct gccgctgctg
     2461 ggcggcgact tcttcgctgt cggccggcag cagcttgatc acccgtggac ggcactcttt
     2521 cagaaacgga atgacttttt cgttgacttc ccaccactgg tcaagagcct tctgctgttg
     2581 gcgccagtcg gcggcggccg ggtcgatgcg agcggctgcc acggcggcca gttgctgggc
     2641 gcgctggacg tgcatctgat ccacgaaaga acccatgtaa tagccgggca cgaagagtgc
     2701 agcggcaatg atcaagagca cggcgaggcc gaacagcaac tggcacttgc gggccaggga
     2761 gatctggaac agcgaaagca gcctcatgcc cggccattgt acacaccgcc cgtgaggctc
     2821 gcactgcccg gcggaagatg gcatcggagg ccggcggtgc gtagactggt atcgctttcg
     2881 accggccagt tggggacgag cgggctcggc cgggtcgagg ctgcgccggg cagggcggcc
     2941 gcaagtaccg cctgccgaat cggcgagagt cgtcaaccga caagggcacc aggagctcat
     3001 gcgaaagcga agggtggcgg cagcctgggg gacatggctg atggtcgccg gggtggtcgc
     3061 ggccacagtg gcccatgccc tgtactacca gagcgacctc tatcgccggg ggctcgaagc
     3121 cgatctggtc ggcttcttcg gactgcctac tgaggtcggg agcatctccc cgcacagttt
     3181 cacagctcgg cagcttgacg atgtccgcgt ctggctgccc gagaagcggg acctcgtttt
     3241 tcggtgccca cgggtgatct gggacaaatc gggcgctccc ggcgggcacg aggcatcgct
     3301 cgacatccat gagccggtgt ggctgctcgg ctcagaggcc tggcgatcgc atgactatac
     3361 ccgcctcctg caggccggtc tgaagcacaa cttccaggcg gtgaaactcg ggatcgtccg
     3421 actgcacggg gcccgtctcg aatggcagtg gccgggctgc aatctgcacg cggatggagt
     3481 cgacggcaag ctggttttcg atgttcaggg aagcgggcac gcggatcttg tctgtcacca
     3541 gttcaacggc catgtcactt ccgctcccat ccacatctcg gcccggatcg atcccgaggc
     3601 tgacgacctt ctgcctgagg ttgtgctggt ggttccggaa ctgcctctga cgtcactggg
     3661 gctcgaccag atactccaat ccccagtaac ccaggggtct ttctgcggga cgatcacgct
     3721 gatgcagtcg cgagggggcg accggatcgt cttgtcgggt cgagcggaaa aggttcgtct
     3781 ggatgagtgg acgaaacgaa tccccggcgg tgccgtctcg ggcctgctcg atctgacgat
     3841 cgaaaaactg gtcgtgcagg accgccagtt gcgagaattg cggttcagag gagaagccgc
     3901 ggagatcgcg gtggacccgc tactggccag gttcggatgg ccgcagcttg ggggtatcgc
     3961 gaggcttcgc gtgctggatg gacaggtcgc tgagggcatc gtacgcaacc tgcacgtgct
     4021 gggaagctgg gacggcggct cgcttgacgg tttgtgtcag aggcttttcg gaaagacggg
     4081 gctggagggg cgactgcgag cccgcatcaa ttcactggtg atcgaaaacg aacgcattgc
     4141 cggcggcgac atcgagctgt ccggcgagcc tcccgccggc cagaccggga cgatcaagaa
     4201 ggctttgttg acggaggcat tcgagaaggt gttcggtttg tggctgccga gccagctttt
     4261 gcctgatcgt gttcaataca cccagatggg agtcagaatc ctgatcgatc atgaggaagt
     4321 gcgcgtcctg agcgggctgg ggccggccgg tcctgccctg atcaccgcga ggattctggg
     4381 ccaggatctg cccttgggcg gtcagttgga cctccatctg agcattgatc agcttctcga
     4441 tcaggtcaaa gggcagctta atcttctgcc ccccgacctc cgcagacggc tcttgacggc
     4501 gacgagaccg acttgaacta tcgcagatgg gtcgtagaat cttgcggtcc cgggcaagag
     4561 ccggtttgag cggggtgtcc ggctgttggc tggtcgggga ctgcggaccc gccggcttcc
     4621 ccttcaaaca cgggtaaaac tctgggcttc cggctaagtc cgtcggcctt cttgcgggtc
     4681 gcaacccaag cgcacggcga tccagagaca tgcgcgcaag ctgcacggat gtcgggccgg
     4741 aaggttctaa cgagtggaga gcgactgatg gcaaaacagc aagtgggtct gatcggcttg
     4801 gcggtaatgg gcgagaacct ggctctcaat atcgagagcc gcggctactc gatggccgtg
     4861 ttcaatcgaa cgtacaagaa gaccgaggac ttcatgaacg gccgggccaa gggcaagaag
     4921 ttcatcgggg ccaagaccct ggaggagttc gtggacgccc tcgagaagcc tcggaagatc
     4981 atcctgatgg tcaaggccgg ccaggcggtc gacgacttca tcaacatgct ggttccgctg
     5041 tgtgacaagg gggacctttt cattgacggc ggcaacagcc atttcccgga cacgatccgc
     5101 cgcaacaagg acctgtcggc caaaggcttc ctcttcatcg ggaccggcgt ttcgggcggc
     5161 gaagagggtg ccctcaaagg cccgagcatc atgcccggcg gccagaaaga agcctacgac
     5221 atgatcgccc ccgttctgac ggccatcgcc gcgaaggtca acggcgagcc gtgctgcacg
     5281 tacatcggtc ccgacggagc cggccactac gtgaagatgg tgcacaacgg catcgagtac
     5341 ggcgacatgc agttgatctg cgaagcatac gacatcatgc gcaaggccct gggccttgag
     5401 gcgaaggcct tgcacgaggt gttcaagaaa tggaacgccg gcgatctcaa cagctacctg
     5461 attgaaatca ccaccgacat cttcaagcga atcgatgacg aaaccggcaa gccgctggtg
     5521 gatatggtgc tcgacaccgc cgggcagaaa gggaccggca agtggaccag cgcctcggct
     5581 ttggatctgg gcatcgccgc cccgacgatc gccgaggccg tgttcgctcg ttgcatctcg
     5641 gcgatcaaga aggagcgcgt cgaggcaagc aagcagctca gcggaccgtc ccctggctac
     5701 aagggtgaca aggaggcgtt catccaggcg atccacgacg ccctgtacgc gtcgaagatc
     5761 tgctcgtacg ctcagggttt ccagctcatg cgggcggcca gcgaagagta caaatggtct
     5821 ctcaagtacg gcgagattgc gatgatctgg cggggcggct gcatcatcag ggcccatttc
     5881 ctcaatcgca tcaaggaagc ctacgaccgc aacccgaatc tggccaattt gctgctcgat
     5941 ccgtatttca agggtatcat cgacaagagc caggcgaact ggcgcaaggt ggtctcgacc
     6001 gcggcggatc tgggcattcc ggtgccggcc ttttcatcgg cgctggccta ttacgacagc
     6061 tatcggagcg agaagctgcc cgccaacctg cttcaggccc agcgagacta tttcggggcc
     6121 cacacctacg agcgcattga caagccaggc gtgttccaca gcgactggac gtaggcacgc
     6181 gccgttgctc cataggtgcg tcggtcgccg agccgtccag cggccggctg ttgcgaacct
     6241 gcgtcaggtc gcgaacgaac aaaatgccgc atcgggtggt tacggagagg gggagtctgc
     6301 ttgttcctcc ggcggcgcgg cgggagcggc attcggatgc gagatgccca gcgccaggcc
     6361 ggcggccctt gcctcgggca cgaccttgcg aggctgggtg ccgtgaaaga gcagcgcgaa
     6421 gatcgccgcg cagcacgcct ggatgctcaa gcccaattcg acgctgccct ggaactgccc
     6481 ccatatcaga atcatggttc ccaccggccc gcacgccgac agtccggtca caatatcatc
     6541 tagcggctgc atgccggaga gaccaaaacg gatgaagtcg agagcaagag gaccgatcca
     6601 cgtgcaaagg acgaaaagga agaccatcac ggccgacttg ccggtaacac ggtaaagaat
     6661 gcgcatgaga tagctgattg tgatcagatt gagcgcgatc gttacggccg tgtatcccag
     6721 attccgggtc atcgtggaag tccagggcga gtgatacacg cgcatttccc ttgcgatcag
     6781 ggccagggga atcgtgcaca gaatcaccgt tacagccaac accatgatat tgggcgacag
     6841 cggtcttgaa ccgatcgcca cgtcccgcag gagccgtcgt tgatggtgcg ccgccgccag
     6901 ccgcgcgctt gccatcacag gcacgattgc gacaagaacg ccgagcagtg tggaagacac
     6961 aatctgaacc gcggggggca gcgggtcccc cggaccgagc gatcgggggc cggcgctgcc
     7021 gaaaaaccgg tccatgctga cgatccccag ccatgtgagt gttgcccaga aggctaccaa
     7081 tgccagcccc atgagcggag tgaatggcag gacgtcctca cggtggaact tgcgagcggc
     7141 gccgacgaaa cagaacatcg ccaaggcggc ctgtgcggga agcgagacct tgaaatcgat
     7201 cgacggacca gtcccggaga acgctgccga caacgccgga gtcagcagga ccgtcagcgc
     7261 cggcaggagc gagacgatca gtccgccggt cgcaatcgcg aaaatgataa acatgacgag
     7321 cagccctgtg gccgccggcg tcagaaagga caggagtgcg atggcggacc aggctagaac
     7381 ggcaaaggcg ccgagagcgg cgttgtccat gagccaatca agctgagacc cgctcccgcc
     7441 ggcggtggtg aacaggccga tgatcaggtt tgccaccgca atggcgattg cctgcgaggt
     7501 ggaaccgacg atgtagccgg tcacggcttg tgggcctgag acgggcatga gccgatgcga
     7561 ctggatcatg ccggaggtca agtccttgcg gatggaattg cggacggcat tgccgccgac
     7621 cagcaccagg gtgactgcct gaagcacgat aagcacatcg gtacatctgt cgaggctcca
     7681 tgcccggtcc gatgggctgc ttcgcaacgc gagcaggatg acggtcgccg ccaggataac
     7741 ataagcggca acagtcaagg cgatgttccg gccgccgccc cggatcttgt agtgagccca
     7801 actcagtgcc ccgccgaaca tcacctccgc tcctttcagt cgacctgcct gatgccggtt
     7861 cgcaggtagg cttcttccag atccggcgtg ttgggtgcaa acgaggccac aggcaatttc
     7921 agcctgatca actggagcag aagcccggct gcgtcttcac gatgacgact gaactcgacg
     7981 atgaaacggt cgccgtccac ttcaaccgct ccgattccct cgatttgtga caggcggtcc
     8041 ttcaggtcgg gcaccggcct caccagtatc acggtgtagc ggccggtttc gccgtttccg
     8101 gcattggcca cctgctcgac cgtgccgtgt tgcacgatgg agccgtgagc catgatcgcg
     8161 atgtgggtgc aatactcatt gaggtcggcc agaatgtgcg aggagacgat cagcgccttg
     8221 ccctggtgac ggaggctgca tagcagtcgg cgaaactggg cgcgccctgc cgggtcaagt
     8281 ccggcggccg gctcatccag caagaccacc gacgggttgg gcaacagcgt gcgagcgatt
     8341 ccgacgcgct gtcgcatgcc tcgcgagagc tctttgatct tctggttctg tttctccgtg
     8401 agccagacct gatcgagcca aaagtcgatc cgctcatcga cggtttcggg gctgatgtgg
     8461 tagcctttgg cgatgaaccg caggaactcc cggacggtaa gctgatcgaa gagccagggc
     8521 gtatccggtg taaagccgat gtggtgcttg tagccgaccc atccgggctc gacgcgctcg
     8581 gccagaacct cgatccggcc ggtgctgagc ggctggaggc cggcgatcgc ccgcaacaaa
     8641 gtcgtctttc cggcaccgtt cggtccgatc aggccgacca ggtatccggg ccgcaatgtc
     8701 agtgagacat cccgaaccgc gaccagcgaa ccgaattgca cacggacacg gtcgacccgc
     8761 gccaggacgt cctcattgtc cgccgtggcc accatcagcg cgccccatga aagagaacct
     8821 tgccccccaa gtatacttca aacggggttt cgagggaaga ccggaggaca tcagaaagac
     8881 ggcggctgtc acgcgttctt cttcgaggga tctttcgcca attgacccgg cgagagacgg
     8941 acttgagtcg tcgcgcagcc cttgcggcgc ttctcgcagg cacaggtgat ctggccgcgg
     9001 aggaagcgaa ccaccagcat ggcgaggaaa agcgtcgccg ccagcaggat ggcgacaacg
     9061 agagcagcgt caatccagcc cgaggcaagc agcgtcatgt tgtggttcat gggtgatgcc
     9121 tccatggccg gctacccgat gagacgaccg atctgataga cggctgtcgc gacgatgaat
     9181 gccagcacag tcgtatacgc catcgcgaac agggcccacc tccattttcc cgactcgcgc
     9241 cggatgacca tgagcgtggc cgagcaaggt gagtaaatca tgacgaagac catgagggcc
     9301 agggctctgg tcggcgacca actcggatcg gcagccagtt ttgcggaaag accttcggcc
     9361 cgtcgggcac tgctgtcatc gttggcgtgg atgatcccca tggtaccgac cacgacctct
     9421 ttggccgcga atccgccgat caatgccacg ttgtctcgcg cgtcaaagcc cgcgtaacga
     9481 gtaagcggtt cgattgccga tccgatccga ccggcaatgc tgtgccggag ctggtcggca
     9541 ccgtcggcgg tattgagtct ggggaacgac atggccgccc agatcagcag gttgacggcc
     9601 aggatcacgg ttccggcctt gcggatgtat tgccagaccc gctccccggt gtgggtcagc
     9661 acgccgcgga gggtcggcac gtgataggcc ggcagttcca tcacgaacgg cgtttgctca
     9721 ccgtgaaaga cggtcttgcg caggacccat gcggccccga gggcgattac ccatgatgcc
     9781 gcccagagag agaacatgat ctgcggcttg tgactcgcaa agaacgccgc gatcagcatc
     9841 aggtacacgg gaatcttcgc gccgcagttc atcagcggca cgacgagcat ggttacgagc
     9901 cggtctttct cctcacgaag cgtccgggtg gccatgaccg ccggcacggc gcagcctccg
     9961 gcccccagtc cgcctccgac gatcatcgcc agcatgcttt tgccctgcag gccgaagact
    10021 ctgagcagcc ggtcgaggac gaatgcgacc ctggccacgt aacctgtgtc ttccaggatg
    10081 gccacgaaag caaacaaggt cgcgataagc ggaacgaagg ccagaacccc gcccacgccg
    10141 ccgatcacgc cgtcgtgcag caacgaatga agcaaaggca tcggttcttc caggccggcg
    10201 accaaccggg gggcagcgtc ttcaaagagc cactcgatcc accccttcgg gctccggccg
    10261 aagagccatt tccactcgtc ggccactttg aagatcgcca cgaaaagcga gtagaccacc
    10321 gcgagcacaa ggagcggacc gaacacgcga tggcagacca ccgagtcgat cttgtcggtg
    10381 atgttctgcc gcgattcggc cgttgaatga acgcactcgc gcacgatgcc ggcggcgaac
    10441 ccgtaacgtc gttcggcaat gatgatttcg gcgctttctc cgaagtgcga ttcgatcgcc
    10501 gcgaccgccg ccgcagaggc cgactcgatc gcggcggggt tacccgccag ttgacgtacc
    10561 ttctgcaaga cccgcttgtc gccctcgatc aacttgaccg ccagccatcg cgcagggtag
    10621 cgaccgatca gccgttcctc gcgggcgatg acctcggcga ctttctcgac ttcggtctcg
    10681 acctcatgcc cgtagctgac cggtcgtagc ttgttggccg cggggtccgt cgcggtgtcg
    10741 gcggttgcct cgagcagcgc gcccaggccg atgccccggt tgccaaccgt ctcgataacc
    10801 ggggccccca ggagcacgga catcttggcg gtatcgattc tgatcccctg tctcttggca
    10861 aggtccgaca tgttcagggc gatcaccagc ggtctctgca tctccatcag ttgaacggtc
    10921 aggtacagat tgcgctccag gctcgccgca ctgaccacgt tcaccaccac gtcgggtttc
    10981 gattcgatga cgaaatcccg ggcaatcagt tcgtccagcg aataggccgt cagtgaatag
    11041 gtcccaggca ggtcgaccag ggtgatttcg cggccgccgt gacaggcatg gccggttttc
    11101 cgctcgacgg tcacgccagg gtagtttgcg acgctctgat tcattccggt caggctgttg
    11161 aaaatggacg tcttgcccga gttcgggttg ccggcgatcg ctgcagtgat ggttgcgtgc
    11221 ttggtctcgc cacggcgggc ggcgaactcg ccgattcgcc cctcgacgcg ggagcgaggt
    11281 ttgccggtcg attcatggaa aggatgctcg tgaatagaca tcggttacca gcttaccgtt
    11341 gggaacccgg tgtgggcgtc ccgtgagcct taccgtcggc cgccgccgag cgatcgccat
    11401 cgtcaaggat ttccacctga atgtatcggg cttccttcac ccggatcgcc agatcagaac
    11461 ccttcacggt gatttgcagc ggatccccca aaggggctcg tcgtttcagg agcacggtgc
    11521 tgccgcgcgt gattcccatg tccaggagcc tttgcctgat ggccccgctg ccaccgacgc
    11581 gaacaatccg ccctgtctgt ccgtcgtgaa gctcactgag caagcttggc actcggctgc
    11641 cccctgttag acataactca agatcgtcgc gacacttacg agattggcgc agcagcaggt
    11701 cccccgggcc tcccgcctgc ttgcaccagt gggccgcgtg attcgccggc tatgccggca
    11761 cccgattata gggcgagttg cctgcccgtg cgagatgcgt taaggcggaa agacctgcgg
    11821 tcaagcatcg gcttgtcttg cgtgccggtt tctcggcgat cgcgctttct ggtcgcggcc
    11881 tggaagaagg cggttcgcct caggtcaaac aaccggttcc gaatgacatc caagccagga
    11941 aagaacatgt gacccgattc acattgaatc agcttccggt ctcctgggct cgtcagatgt
    12001 tgcagacccc tggggttatc gctgcactgt ttcccgagtg ttgcgggaga aaacgagaaa
    12061 accatgacac ccgcttggtc ggcagttaga atgaaaagac agaggatgag gagggcgtca
    12121 ccgcagagtc cacggaggcg gagcgggtcc ccaccatcag gactgttctg gctggtcccg
    12181 gaacccaacc ggctcaacct atggtcttag gaagccatgc ggtttaccac ggtgttccgg
    12241 acaaggcagg tggagtgttg tcatctcatt tcgctgaagg acgtggcatg cacaagatat
    12301 tattaagggc ggttctgtat cgcctgtttc tgtggacggc aatctgggcc ggctgtctgg
    12361 ccggcggtct ccgggttgcg cgggcggacg agtatcgggc cttctgggtc gatggctggg
    12421 gagcgggctt tctgaatcag agtcaggtgg acacgctcct cggcgtggtc ggggacccga
    12481 acagtaaggg cagaatccgc gaggccaact gcaacatggt gatcgtccaa gtgcggcggc
    12541 gggcggacgt ctgttacccg tcgggcatgg gcgaaccgta tttctcgggc ctgtcaccct
    12601 ccaatttcaa cgccctgcag gccatgatca gcgccgcgca cgacacgaca ggcggcaaga
    12661 agcgcgttga ggtccactgc tggagcgtgg tcttcaagac ggccaacggt ttggtttatt
    12721 ccctccacag cgacccggcc aatcccgata actactggcc gacgcgattg ggaagcacga
    12781 cgggttctga gaactccgac ggagctttcg accccgggca ccccaaatgc cttgagtacc
    12841 tggtcaacgt gcacatggac ctggtaaccc gctttgatat cgacggcatt cattacgact
    12901 acatccggtt cgagggcaac acggagggct ataacccaac cagcgtagct cgctacaacg
    12961 agcgttacgg tctgagcggc gacccgtctt cgagcagcga gcagttcaaa caatggcggc
    13021 gcgaccaggt caccgccttc gtccggcaga tgtacgcccg tatccaagcg gtcaaaccgc
    13081 atgtgaagca atcgggctcg ttcgtgacct ggaacccttc gccgacgtcc agcacgcggg
    13141 ccgcattcat ggccactcgc ccctattacg acgtctattc ggactgggac agttggatgg
    13201 aggaaggcat tgtcgacatg gccgtgccga tgacttacta cgactgggcc agtctaccga
    13261 acgactacac ccgctggatg aacttcgaga aggatcgcaa gttcaaccgc cacatgattg
    13321 tcggtccggg gatctacctc aactcgctca gcaacgcgtt gtacgagcta cagcagaccc
    13381 gctgggcctc gccggccggc aactacgcac agggcttcag cggatactcc taccgcgtgc
    13441 cgtacagtgg tggaacctgg gctggtttca gcccttcgct ggttgcggac gtcacgccga
    13501 cctgggatga cgtcccgacg atgccctgga agaccaatcc gacgacgggc cacatctgcg
    13561 gcacggtgac ccaggtcccg gatggcgcct gggccgacca tgccacggtc agcatcaccg
    13621 ggccagtcag ccgcagcatg cacgtggacg gcaccggctt ctatgcgttc atcgatctac
    13681 cgcccggcag ttacagcatc accgcttcaa aagcgggcta cgcaaacgca gtatcctcgg
    13741 ccgatgtggc catcggccag gtgaccggga acatgtacgt ccgagatctt gtgctgggcg
    13801 gcaatccgtt gccggtgatt tccagcgtgc aggccaccaa cgtgaccgac agcggggcca
    13861 ccatcacctg gctgaccgac ctggcttcat cgtcgcaggt cgaatttggt ttgacgactt
    13921 cgtatggaaa cctgacgcct ccggatagta cgccggtcac gtcgcactcc gtgacactga
    13981 gcggccttgt cgccaacacg ctgtaccact atcgggtgat atccacgaac gccaacgggg
    14041 cgagcatttc gagcgactat tccttcaaga ccgcagcttc gacgacggag ttggtgatcg
    14101 acaacacgga tcaggaatgc actcgaacgg gttcctggaa caccggtagt attgccgacg
    14161 tccccaaggt gggcacgaat tatctttaca cgcccggcac gggcaacggg ttgtccgtca
    14221 cggcaacgtg ccggtggacg ccgaccattg tgagggccgg gctgtatgac gtatacgttt
    14281 tctaccagat gggttcgaac cgcaattccg cggctcctta cacggtggtt tacgacggcg
    14341 ggcaggttgt gagcatccag aaccagtatt cgagtacgcc aaaccagggc ggatggtttc
    14401 tggtgggcga gaacctgccg tttgcggcgg gaacgggcgg ttacgtcgag tgtgcgaata
    14461 acagcacgga caccaggtac atcagcgccg atgcggccaa gctcgttttc aagagcggca
    14521 tcgagctgcc gcccgcgaag gcggccggtc cgaacccgtc gcatcaggca acgggcatct
    14581 ggacccacac gggactgtcc tggacggccg gggagggcgc gacctcgcac cgggtctatt
    14641 tcggcacctc gagcccaggg agtttccaga tagagactgc cgatgcagtg tttgatccgg
    14701 gcgcgctgct ggcggcgacg acctacttct ggcgaatcga cgaagtcaac gcttccggca
    14761 cgacgaccgg tgacgtgtgg gagttcacga cccgccagac cgttgccgct gatctggacc
    14821 gagatggtga cgtggatgcc gccgatgggg atcttttcga gtcgtgcgtg tccggccccg
    14881 gcgtttcact cgatgaaggc tgtgccgacc gtgacctcga cggtgacggt gatgcagacc
    14941 agtcggactt cggcgtcttc cagcgctgtc tgagcggaac ggatgtaccg gtggacccgg
    15001 gttgcggagg ctgatcagac agcggacggc gtcgttgggt cgagaagaag gcctcctttg
    15061 tattctgccg ctgaggaagg caggtctcct ccgtctgcgg cgtctccgac cttgccctac
    15121 ccttttcacg agtattccca gcctgcgccc agccgtgggt cttgctcgca gatgtcccgg
    15181 cctttgcgtg aagccgggaa atcgctgatc atgttgtcgt ctgtcgggga taagcaattc
    15241 atgtcctgtc cctgaccgat tcagaaggga gtgccgcatg ctgaacaaag tgctggtcct
    15301 gtccgcatcg gcgggcgcgg ggcatctgcg ggcggccgac gcgatcctca aggcgatcca
    15361 tgaactgcga gcggccaagg aggctcgcca catcgactcg ctcgactaca ccaacaaggc
    15421 gtttcgctcg ctgtattcca aggcgtatat tgagctggtg aacgctgcgc cggacgtttt
    15481 gggatggctc tatgatgcgc tggacaaacc ctggaaaaac gaacggcggc gactggctct
    15541 ggacaagctc aacacccggc ccttcgtgaa aatgctggag gagtaccaac ccgatatcgc
    15601 ggtttgcact cattttctgc ccgcggagat catctcgtgg ctgaaggcca agaaacgatt
    15661 gcgatgccgc caggcgatcg tcgtgaccga cctcgacgtt cacgccatgt ggctgtgcca
    15721 ccattatgag cactactttg tggcaatgga tgagacccgc gagcacctga tcactttggg
    15781 catccccgcc gagaacatca ccgtgtccgg catcccgatt gacccggtct ttgcccgccc
    15841 caaagacaag gtcgagacgc gaatcaagca tgacctcgat cccgacaaga ccaccatcct
    15901 catctcggcg ggtggtttcg gcgtcggccc catcgaacac ctgatgatct cgcttctgaa
    15961 gttgagacac acggtccaga ttgtcgcggt ctgcgggcga agcgaagaac ttcgcaagcg
    16021 gctcgagcgt ctcgtcgtcc ggcagccggt cgacagccat attcgcgtca aggtggtcgg
    16081 gttcaccact gagatggatg agtatatggc cgccgccgac ctggtggtgg gcaagccggg
    16141 cggactgacg acgtctgagg ctctggctcg cggactggtg tatgtgatcg tcaacccgat
    16201 cccgggtcag gaggaacgaa acgcggatca tctgctcgag gaaggggtgg cgattcggtg
    16261 caacaatctt ccagtgcttg gctacaaggt tgatcggctg ctcagcgaac cagggcgtct
    16321 ggcggccatg aaggccaacg tgcaacgtat cgcccgtccg cgtgcggcct atgacatcgt
    16381 caacaagctc cgcgagctgg ccgtagggtg agagtggcct ctgaaacagt gagggacggg
    16441 attcaaagac gtgctcggtg agaatgagga tgggaattct ttagggggag ggccccagaa
    16501 tggattctgg ggccggtggt ggtggattct cggccggtgt gtacgccggc ggcggagtgg
    16561 ccaagtcggc cggcttgtcg ctctcaggtc atggatttcg ccacttgaat tggatctcga
    16621 cctcctcggt cttgccttcc cgctcgtgct cgacgctgat ggttgcatgc ggcgggatgc
    16681 gaacccgctc gccggcgatc tggatgcgga aaggcaggtc cttttccagg cacgcggcca
    16741 tgcggcggag ttttgcggcg aactgcttga cagggtatgc cttttcgata tcccgatccg
    16801 agcgttttcg gcgtttagcc atgtcgttgt catcctccct gccggtccgg cctctttccc
    16861 gaaggacgta tcagatccgg accgacccta taggctatct tcattccgca ctcaacggtt
    16921 gtttcttgga agtcctgtcg cggctttgca ggacggcctc ggctcctttc tggctgatag
    16981 tcctgatttc atgggcgctg agcagctggc caaggacgag gatgtcgtcg acgctgccgc
    17041 gaagctgttc ctcacggccg tgatcggcgt cctcgcccac gaacaggttg cgtgtcattg
    17101 agatgggctc gccggccgga acgcgaccac cgccggccgg ggctccgtcc agatagaacg
    17161 taatcaggcc atcatcgtag gtcacggcca ggtgatgata cttgccgtca gcgaatcgca
    17221 gaggtttgga ctccaccgcg atgcccttgc agatcagacg caagccgggg atgacctggc
    17281 ccttagggtc gcagtcgaag accagctccg ttgtcttgac ggggccttgg tcgttggtgg
    17341 aactgatgag cctggcaggc ttgctctctt tgttctttgc catgagagcc aacgtgaatt
    17401 gtgtcccgag gttcttggtg cctttgatct cgatcgtgtt cagcgggcac catggagagc
    17461 caagcgggca gccggcgctg ccgaacgcgg ccagttcggg atcggttttg gggcggattt
    17521 cgccatgctg gacggcctgc tgcttgccat cctccctgag agagtcggtc cagccgtctt
    17581 cgaaggtgta cagaacggcg agggttgggc cggccgcctc atcaataatc tgaagcttcg
    17641 gcccggcggt gaaggaatac agggacgcgt tttccatgac gaaccgcaag cgaatcggcg
    17701 cgtcggcgac cggcagctcg ttgcggttgt tccaggtgac cgtctggtcg aggctgtcgc
    17761 cggtcagagg ctcgcagtcg tcgcgtgtgt atccggagag gtgcttgccc tcagcttcga
    17821 gcacttccac gcgaacggac cctcccgccc ggcactggta gttcaaacgc agcggtccgg
    17881 cggttccggc aagcttcttg gtcaagatga ttcccgtttt ctcgcccgcg tcgagtgatg
    17941 cgaagccatc cttgcggagc gtcgccagtc cgatggagcc gtgccatggc gcaggcgcgg
    18001 cgtgcgtgca gtcgaacccg ccgtagtaga gccagagctt gccgtcaacc accagcgggt
    18061 gctgcggcgt gtagtgcatg ccgtcgtccc acgtgccgtc cttgccgagt tcgagaatcg
    18121 gcgggcgctc accttcctct cggaaccagc ggacgccgtc acggctggtg acgagttcga
    18181 gatgcaccgg tccgtcaaag tagccctcgt cgtcggtggc tcggaaaacc cacagaaagc
    18241 ccaggtacat cgattcgtat gcaaaggcgg acaggccgta gaagtgcgtc cgctggatgc
    18301 ccttggccca tcggtcgtcg aaagtgtccg gagcgaggac cagctgtgga tcggaccagg
    18361 ataggatgtt cttggaggcg gtccgggcga cggccctgcg gcgcatgccg ctgacatgga
    18421 cgttgttctt gacgaagccg aggtactggc gggtgtggga atcccaaagg aactggctga
    18481 cgtctccgcc cttgctgaag accggcttgt cggacgcatc cgtccagtgc agaccgtcag
    18541 gggagaaggc acagacatag ccgttcttgg cggggtcgcc gtcgaagttg atcatcttgt
    18601 agcgacggtt ggggtccttg tcccacggcg tatggaggac cgacaggata tggtctcgtt
    18661 ttccccgccg gataaagatg ttgttgtctt tggagccctt gtactcgacg atgccgaggt
    18721 tcggcttgtg ccacttgatg ccgtcgtcgc tggtggcgta gagcagccgg taggtatcgt
    18781 cgtctccggg cagggcatga taccacatcc ggaacccacg tccggattcg ttcggcaaca
    18841 ccgtcccgta cagatatacg ttcgtgccct cccacggccg gtcgtatgtc aataccgggt
    18901 tgcccggatg cttctcaaag gggtggtagg tgcgcgccac gttcgccttc tcggccacgc
    18961 agtaatcgtc gaccataagt tgccaagggc cggtgagatc attcaacgac ctggtgtccc
    19021 aagccgcccg gacacagggc gcgagcgatc cgaatagcgt ggcgatgacg aaaaggtgag
    19081 agtcgttgcc catgctgtcg ccctccaaaa gcagccacgg agttgattct atggcccgtg
    19141 ccaggatgcg ccaaacatga caacacctca cggccggttc aatccggaca gcagtcattt
    19201 gcctgtggtt gaagagggaa tcactgcgga tcggtactgt caggttaaaa aagccaagcc
    19261 atgtctatca gctcttttca ggcgtccgca ataaaaacaa aaccagtgag tgctgtaacc
    19321 aattgctgct gttcgagtta taaccggaca atagtgactg ctgcggatat gcattccgat
    19381 ctgtcgattt gtgattgtga ctgccccata cagcaaacac ctatttgccc tcgcaccgtc
    19441 aggcgccgga ctccaggcgt cttacgccga gccattggat gttggtctga aacaacgcct
    19501 gtcgtttatt ggtccgcagg ttgacctcgc cttggtttgg tcaactgcca cgagacccag
    19561 tcttcctcac cactgacttg tagcttctcg ccggtgcgat cgttctgaat gtaacctgcg
    19621 ggcggcttcc actcgaatac ctcgcttgga atgttgcagt tgacagctgc gctgattatg
    19681 ttgatatcgc gagacgtcag gagtttgccg tcttgatccc atgcttcgat ccgcaccttc
    19741 tttggcacgt tcgctttgtc acgatcctca taacgggttc gtgatataag gtgttcgccg
    19801 tcatacaccg ctacttcctg cacgcgagaa tagtccacgt ggtcaacaat gaagacagta
    19861 cgtttggccg gattgtcctt ggacctgaat tccagactgc ttaatgatgg atcttcgagc
    19921 ggacgtacgt tgacttccac attctggtcg ccagtgctaa cgaacacgga gagcttctcc
    19981 cgatccagta tcagctctct tgtcgccgga ttcccgggtt ttccgagtag agcggccccg
    20041 aatatcctta atgacgccgg cgttgcacac caatcgtcca ggtcctgctt cgtcttccgc
    20101 cacggtttgg cctgtttctg aaccaaggca ctgcgtctcg cgtgatcata ccatatgagc
    20161 ttcccgcgtg cactgatacg agtttctgca aagacatctg tgaccttgaa tggctcgccc
    20221 gattccagga cagtctgatc gatgcgtgtc tgctcgccag agattcgtat gcgcgctact
    20281 cggcgatgag gcttcatgtc ctgttgagct ttgtggatac gttgaacctc ctcttccagt
    20341 tcagtctctc gctggggtgt tccttcccga ttaacggcac ggttgtcagt cgcgtcatat
    20401 accttggtta ccatttcacg aatcacttcg atcttctggg gcggcacaac gacggtctcg
    20461 cagtaggtaa cgtcgtagct gtccgggggt cctcgaaaca cagcatcggc aatagactga
    20521 gccagaatgg cccgacccgt atcgtcgcac cgagcgatct gttgagaaaa cgtgctcagt
    20581 gcaatcgcac atgccgtcag taaatgtcgc ataccgacct cctcgtattt ggaatgagaa
    20641 accaactctt cagtctcgca ttgccgagac gcatggcaga tcaaggttcc ccttggcacg
    20701 cttcaccata ttgctccttg aatacatttc gcatcacatc ctgctgaata ccattcggct
    20761 cgcagccttc gagaagatcg catcccgagc aaaggccgcc aaaagcagcc tgttgtgcca
    20821 ggcactccgc gcatgcataa cagttcacgg gagtgggcgc cgcgatgcag gatccgcatg
    20881 tgaccacgca ggccgtcgtt gtcgccgcac aaaagaccac ccaatccaca ttccagttgg
    20941 cctcacacat ctcgaacgtg ccgattaccg ctccttgttc atcacatccg gtgtatccga
    21001 cctcccccaa tccagccggc tcacaagatg tccattggta ggttctgtag actcccccaa
    21061 tacaagcggg ttcatttcct tgctcaatgc attccgggca ttcatcatcc catacggccg
    21121 gctgggtcgt gggtttctgg cagtcgtagg agttaccccc ctcactccgt cggtcggcgc
    21181 tcagaagcga taccagagta agcgcgacaa tcagggcgtc tctattcatc gtgctctcct
    21241 ttccggcgct tcgcaggcta ctgcgtcggg ctgcttctcg tccaactcca ttgggctggg
    21301 taccaccaaa gcagacgact ccatctctga gatggcctgc tcgacctgcg gaacagcatc
    21361 tgctcgcaag cgaacaactt tcagaccctt aacaaatggg aatcttctag tgaactggag
    21421 taacgactca cttcttgccc cccttggcac tgaagagcag aacagcggtt ctctactctc
    21481 tggggcaagc aagatgattc ccacgtggtc gcactcaagc cacggtttga gtccgggcga
    21541 tgatcgctct ctcgaatgat caacaatatc accctcacga aacaacggta aggcctcggc
    21601 cagttctgag acaggaaggt agcaatcctg ccgggtttct ttttctggga ccgatgatac
    21661 cttggctgcc tgtcgctcag ccttcgctct tcccgctgga gtctcgtctt gtccgaatcg
    21721 aaggtttgcg tagaactgct tgcggaacac ggtgtattcg aattcctgcg tctcacccag
    21781 agtgcgagtc acatcctgaa ggagccaact gttgttgggg acccagtctg ccaaagtaaa
    21841 aaagttacgt tccaaaaagt ctattcgccc ctctttgtga cggagccggt tggccaacag
    21901 ccagtatgtg cgccaatctg aggcgcatgc ggccgcaaga gttcgctcaa cgaatgtaac
    21961 acagtcgcca caggacagat tgaacatgcc ggctcccaat ttgtatggtt gtccttgtgt
    22021 ttggcgtgcc aactccgcca cccttgtcca aggcggtgct tcgggatcgc atttttccct
    22081 gagattcagg tattcagcca gatcggagtt cgtgaacaag tgaagggggc gcctttgaag
    22141 ggaagcgaat cgactcatct cctcacttgt cgcagccgta gcgccttgac cagcaacgac
    22201 actaggtcgt gttgacattc aggtgatcca acaaaagagg gcggcgagtt ggacga
//
LOCUS       DAOTPW010000074        22244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2401_length_22244_cov_5.315381, whole genome shotgun sequence.
ACCESSION   DAOTPW010000074 DAOTPW010000000
VERSION     DAOTPW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22244
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_22244_cov_5.315381"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..853)
                     /locus_tag="P5316_08615"
     CDS             complement(209..853)
                     /locus_tag="P5316_08615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRR85058.1"
                     /translation="MTPTPSTELIIVRHGETEWNLDGRMQGHADSNLTPRGFRQAEAV
                     AARLVQERIDAIHSSDLGRAIRMAEVLGRAKGVTVIPDARLRERNMGIFQGLTVEEMN
                     RRFPVEYDRFASRQVDYVIPDGESMRQCHDRVITGLQSIADRYPGRRVVVVTHGGPLS
                     AAFRHTMQLPINAPRRWSLFNASMNRFEVQGTRWRLLVWGDLTHMNGLASQDDF"
     gene            complement(933..2210)
                     /gene="aroA"
                     /locus_tag="P5316_08620"
     CDS             complement(933..2210)
                     /gene="aroA"
                     /locus_tag="P5316_08620"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRR85059.1"
                     /translation="MRLVCLRSRLSGAVDIPGSKSHTIRAITVAALADGESRIESPLI
                     SADTLAAVDAYRALGAEIDATRPESWVVHGFSGRPNAPANVIDVRNAGTTLRIATGSA
                     ALLSNGLAVFTGDHQIRRRPIGPLAQSLNDLGASVTSTLGNGCAPLVVGGRLRGGKTS
                     IEAVSSQYLTSLLMACPLADGDSEIDVPLLNEAPYVEMTLDWLKHSGITLERDELRRF
                     HVPGGQQYRPLSRRVAADFSSATFFLAAGALGDNDVLVRGLDMNDPQGDKAVIDYLRL
                     MGARIDVQPDGIRVRPGELKGCEIDLNATPDALPMMAVVGCFSFGKTSLVNVPQARLK
                     ETDRIAVMAAELSKMGAVIRERPDGLEIEQSRLRGAEVDGHHDHRVVMALAVAGSAVE
                     GRTIIQTAEAMEVTFPTFVDCMSRLGGRLSLEQ"
     gene            complement(2554..5586)
                     /locus_tag="P5316_08625"
     CDS             complement(2554..5586)
                     /locus_tag="P5316_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HRR85060.1"
                     /translation="MTKPRQIRRSGLSVLVALLSAVPLLVGQGCPPMNWIWPGGDPVG
                     GTGSDSGSGSGQDIPGGTNTPPYFEFTKPLSDVMREVGDIIEIAWLDGDPDDNAVISL
                     YVDPDKIFGNGTEILIESNISENADNSQGFYYLNTTAKGLQPGEYRIIASITDGVNPR
                     ELIVAPGLLMLFPPGMVPGNLSPAVVAREPSVNYSVGHNSEVPISWCVSDPDDGENQV
                     LPDIVILLDLDQNPLNDLVFSGSNAERNLAEACFAVQDPLDGPYPVMVDGELRAYVLG
                     CFKDTVGGTLGECSNPTTPGGSFPPNGTYTINAMKLPPRVNGEPYRVRVTAWDHTNPP
                     VSRYAWGGVTVSSTATGNAGLVDLAEVGRTIRGAKFYGFDENGQSGYTGVGVGDMSGD
                     GIDDFVIVSRYGRGYGMDAMGSAHLVLGMAGQQFGGDIPLNSITTTYAGALFTMPYST
                     KSQGLVSVARVGDVTGDNLSEIVFGAPYTEVFYDRVDDDPCDSSPPSCYFDYMPNPYS
                     DQPPDNDEMGAYDWHEDLIVPQPPPDPPPPPYLCSNDEDLFVQTPIASGYAIVVGSNN
                     SLNNDIYGLEDVGQALGFNGARFRGAYFDDLDIPGTLTGSVNRTWPYSIIPDNLFGLT
                     VNSMPSISDSTPGLTARWGPSLVISSPKNGRNRGRVWYFEWFDYTSRLPGSTTPDIQS
                     FPALVGCGFRTRIIPGGTDGTGRSGSQVITGEMAGDLFGYAGPAGDFNRDGSQDIVAG
                     APGADRNGLIDNGIVYVVFGRPDFDNPDMTNLEEFDLDIWNSPRLEIHGDRNNDRFGE
                     SQTLIGDVNQDGHPDIAFASQYAGSDGIGGPESGFIGIVFGGRHFAGVNKFNVGHVGG
                     PTLPGVRIYGRQAGGHAGAVINDVGDFNADGIDDLVIVAPDEIRLINGLRRQGVAYLL
                     FGGPHLANKTINLSQVGSTVPGLVFVTPYGMTDANAARITWASGAGDVNGDGFDDILL
                     GLPEADTVYPYNPSLRKVDTGEMYLIYGSNSGTNGMSW"
     gene            complement(5815..6504)
                     /gene="rpe"
                     /locus_tag="P5316_08630"
     CDS             complement(5815..6504)
                     /gene="rpe"
                     /locus_tag="P5316_08630"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRR85061.1"
                     /translation="MASNNDWPLAQPRIRMAPSLLSADFARLKDEIQAIEAAGAEVLH
                     LDVMDGHFVPNISFGVPVIEKLRPVSKLFFDTHLMISDPVRYAEAFAKAGSDLLTFHI
                     EVAPEPMAIADHIRSTGARAGITLNPDTPASALRPVIGAMDLVLVMSVWPGFGGQQFI
                     ESSLDKLRELRKLLRPDQRLEIDGGIGPLTVQLAVQAGADTLVAGSAIFGQPDPAAAM
                     RDLLRLAETRG"
     gene            complement(6605..7843)
                     /locus_tag="P5316_08635"
     CDS             complement(6605..7843)
                     /locus_tag="P5316_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRR85062.1"
                     /translation="MAKKTIADIDVKGKTVLMRVDFNVPQDDAGNITDDRRIRMAMPT
                     MQHVITNGGKVVLMSHLGRPEGKDRAADAKYTIKPCAERLGKLLGKPVRFVPDCVGPE
                     VQKAVAALKPGDVLLLENVRFYEAEQIKDKKAKEDPALAEKKRSFAKQLADLADVYVN
                     DAFGTCHRDNASMLTVPQLMGGKPKVVGFLVKKELEYLGDALNSPQRPFVAILGGKKV
                     SDKIGVIEALLKKCDTILIGGAMQHTFMLAQGRKIGKSLIEAEKVEEAKRLLQLGGSK
                     LKLPVDVVCAAELKPGIATKVVEGDIPDDLSGFDIGPKAIEEYKKIIMGAKTIAWNGP
                     MGVFETKPFDVGTYAIAHALVDATAKGAITVIGGGDSAAAIEQAGLTDGVSHVSTGGG
                     ASLEFMEGKPFKAVDVLDEA"
     gene            complement(8445..9176)
                     /locus_tag="P5316_08640"
     CDS             complement(8445..9176)
                     /locus_tag="P5316_08640"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRR85063.1"
                     /translation="MNPQEFEQTLVLIKPDALKNSVTGFILSQLSEVHTGLRFAAAKI
                     IHVSEMLAAEHYAEHAGKPFFPALIQYIRGYAHYPDEPWKRRVIAIVYHGHNALRIIR
                     QMAGPTNPHRAREEQPGCLRALGTVVTLKDENGKEIGERLDNLIHASANHEDAEREIK
                     LWFTPSDIPPLMQVYPTEVCKEHYYFKDGRLCDTYEPDSVCLLAPGMIAWESDLAALR
                     ALSANKPSARSLNAVAAKYLINRPD"
     gene            complement(9238..9438)
                     /locus_tag="P5316_08645"
     CDS             complement(9238..9438)
                     /locus_tag="P5316_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85064.1"
                     /translation="MSHLGRPEGKDRTADAKYTVKPCADRPCKPLKSVGDCLPCPAAA
                     QGRRPGRGTTTSRAKPGDFLLL"
     gene            complement(9416..9559)
                     /gene="pgk"
                     /locus_tag="P5316_08650"
     CDS             complement(9416..9559)
                     /gene="pgk"
                     /locus_tag="P5316_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRR85065.1"
                     /translation="MPVDFNVPQDDAGNIADDRRIRMAMPTMQHVITNGGQGGADEPL
                     GPA"
     gene            complement(9832..10467)
                     /locus_tag="P5316_08655"
     CDS             complement(9832..10467)
                     /locus_tag="P5316_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85066.1"
                     /translation="MRSAVRAPDPPLLYSHDLSALRAANTELDRRMQADRLGVHEQFA
                     PLSNWTVRVRYDPRRVARQDADFVVQLQGRSVDEVFAPLASQFKVDPQTSFETVTGTI
                     KHVLWTPDHALVIGLFELSQDEHDQSRFGRPVVVRAMRGDAYVPAPEDVIITKLLWYQ
                     RERRSKGLNDARNILATQQKLLDAACRERWCRTHGTLELLPQLREQFPPLA"
     gene            complement(10781..11800)
                     /gene="gap"
                     /locus_tag="P5316_08660"
     CDS             complement(10781..11800)
                     /gene="gap"
                     /locus_tag="P5316_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006616712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRR85067.1"
                     /translation="MAIRVAINGFGRIGRLVLRAAMEDRDIEFVAVNDVVPAENLAYL
                     LKYDSVHGRAKVEVRQEGDNIVVAGRKVKCVSILDPAQLPWKDLKVDYVVESTGKFTD
                     RAGAGKHLTAGAKRVIISAPAKDKDIPTFVMGVNEQRFNPATDTIVSNASCTTNCLAP
                     ITKVVLDNFGIVEGLMTTIHAMTATQPTVDGPSKKDLRGGRAAGQNAIPASTGAAKAV
                     GLCIPEVKGKLTGMAFRIPTADVSAVDLTVRTEKATSMAEINAAMKKASEGPMKGILG
                     YTEEPVVSSDFIGCPLSSIYDATAGIELNANFFKLISWYDNEMGYSCRVNDLMKFIGK
                     KDGLL"
     gene            12603..12878
                     /locus_tag="P5316_08665"
     CDS             12603..12878
                     /locus_tag="P5316_08665"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HRR85068.1"
                     /translation="MVVKNGKTVTFIYRPEHPACRDVALAGSFNDWKPDKGKMTRQKD
                     GTFRKRITLRPGEHRYKFVVDSQWVEDPQAEKQTTNEFGTRDSVVVV"
     gene            complement(13039..14157)
                     /locus_tag="P5316_08670"
     CDS             complement(13039..14157)
                     /locus_tag="P5316_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR85069.1"
                     /translation="MTSRERLLTVLRRGRPDRVPVTIYGYSKHDDAWYNHEPSYAPLI
                     ELESRYGDSFVTAPIDCPVFLGDPNVVHGQEEIHPDGSVVMTTEINTPKGRLRAVSRR
                     DPGLMTNWQVEPLIKSDEDIERLLSMPDPPAEVNAQRIADLQARVGDDGILCFSVGDA
                     LGHVVGLFDFEDFVLRCYTDDGPIRALLDKAQGQVLRTIKAIGPLVRNAAFRLWGPEY
                     AGAPLMDPAVYFARYVVGRDREATEAIHATGNFCVIHCHGRLRDLLDMIADIGADALE
                     PIETLPMSTADVTLAEVKERIGRRMCLMGAIQAHTLETGMPEDVRREVRDAVETAAGD
                     GAFVLLPTSPPFMVPLAVKSLENLRAMYLAAHEFGIYD"
     gene            complement(14181..15572)
                     /locus_tag="P5316_08675"
     CDS             complement(14181..15572)
                     /locus_tag="P5316_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRR85070.1"
                     /translation="MPDIKRIAIIGLDCGEPSLVFNRWLPDLPNLRRLCETGQYGNLL
                     SCTPPITVPAWSCMAASKDPGQLGIYGFRNRADYSYEKLSIATSLAVREPRLWDILSA
                     RGRENIILGVPGTFPIARPPKGLMVTGFLAPDTNSDYTYPKTLKDEISRVVGDYIIDV
                     KDFRTDNKQWLLDQINEMTDKRFKLARHLITTRPWDLFWMVEMGVDRIHHGFWQFMDS
                     THHRYVPGGPFESAIRDYYVRLDRLVGELLEVIDFETTAVWVVSDHGSKCMIGGFCFN
                     DWLIREGYLALKEPPSKPTKFDIANVDWSRTKAWGEGGYYGRCFLNVEGREPQGIIPR
                     DQYEAVRDELICKLQALRDHNGHVMGTQAFKPQDVYKKVNGVPPDLIVIFGNLNWRSV
                     GRVGNDSLYTFENDTGPDDANHALEGMYILSHPSLEGRGRCDDATLYDVAPTCLKMLG
                     YPVPADMIGKPLV"
     gene            15791..16759
                     /locus_tag="P5316_08680"
     CDS             15791..16759
                     /locus_tag="P5316_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRR85071.1"
                     /translation="MAVLTTLYEPIAKDLEKVCQIFDDELFSDLPLINDLCAHVRQYR
                     GKLLRPALLLLSGKACGKTTDVHLTLAAVVEMVHMATLVHDDVLDDADLRRRCPTINR
                     LEGNEAAVLLGDYLISHAFHLCSSLGSQHASRVIAATTNIVCEGELQQVHHRGDFNLG
                     LDDYLQIITRKTASLTGACCRLGAHFAGAGTETAANLERYGLAVGIAFQITDDILDII
                     GSEKRMGKTLGRDLQKSKLTLPVIHCLTRADDTLRKELHLLLAGSNPDRNRLRNLLET
                     TDSINYSLHAARDYIESAIARLDDLPPSQARHSLSRLARLIISREQ"
     gene            17318..17932
                     /locus_tag="P5316_08685"
     CDS             17318..17932
                     /locus_tag="P5316_08685"
                     /inference="COORDINATES: protein motif:HMM:NF037679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRR85072.1"
                     /translation="MCTVWTVQYLIMNETPENGASVSVRDRLLDAAEAVVAREGVSRL
                     TLEAVAHEAGVSKGGLLYHFPAKAALITAIVERLAIRCESRQAEAVASDPQACGAFIR
                     AYLTVRTHPPDPQRESIHTAVLAAIGTNPDYLDPLRRRIAEWQKRLESDGIDQVTATI
                     VRLAVDGLAIGRLLKVPIPEGELRERVIERLLSMTHPQAEEKRS"
     gene            17991..19736
                     /locus_tag="P5316_08690"
     CDS             17991..19736
                     /locus_tag="P5316_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRR85073.1"
                     /translation="MTGHQPVVACSFMVIVCLCCLPSGCSVGPGYRAPTPELPEAWHQ
                     TTTGGPAEENADLQEWWHVFEDDLLSDLIKRAAEGNLDLRLAVLRIREARALRNIAAG
                     GLLPSLAGQGGWQRSRASANGLMGGAVQAPGKGTQFANTMARGIASSALSEGLGTAFP
                     GAPAVTNSLAGGLMGLLPTPTRLAKTEAMDLYTTGFDASWEIDVFGGIRRNLESADAV
                     LAAAVEDYRGVLVSLLAEVAATYVDVRSLQSQIEATQRNIKLQKETVALTQVKFDSDL
                     VSELDVRQAETNLATTESELPLIEAALSAAIYRLSVLVGREPAALYDELSQVRDIPKP
                     PAETLIGVPVDLLRRRPDIRAAERRLAAQTAQIGVAAADLYPRFTLSGTFGFQATDIN
                     HMLDSRSVTYGFGPALRWNLFDGLRNLNRIAAQQAVTHQAYVFYEQTLLQALQDVETA
                     LVAYTREQARYTALVRATEAVRRSAQLAEKRYQDGLTDFQNVLIAQRSLVNLETALAQ
                     SRGQVAVNLIALYKALGGGWSTDAMPQEQFLDDSSGVLADPMGFFFSGGKGTLPWENH
                     LDEQPETGPNSTNPE"
     gene            19801..21000
                     /locus_tag="P5316_08695"
     CDS             19801..21000
                     /locus_tag="P5316_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR85074.1"
                     /translation="MLITDSFTRISLLGFLAAGLNVLRCNTQEAPPRPQGPPEVAVAT
                     MSPEPIELITELPGRVWPYLVAEVRPQVSGIIQKRLFEEGSDVRAGDVLYQIDAAPYE
                     AAYSRAKAALAAAEANLPAVKARVERYKNALAERAVSRQDYDEAVAALEQARATIELR
                     RAEVESARIDLSYTPIKAPISGRIGKSNITVGALVTAHQPTPLATIQQIDPIYVDVTQ
                     STAELLRLKQGMETGRLSKDDENQRRVRLRLEDGTPYPLDGTLQFRDVTVDPSTGSFI
                     LRIVFPNPEHLLLPGMFVRALVREGIVQNAILVPQQAVSRDHKGNPVALIVDAEGKVQ
                     QKTLAADRAIGNKWLVSSGLEPGDRVIVEGLQKVRPGMPVKTVPFDAGQNDGLAEQGV
                     QSAPSAN"
     gene            21014..>22244
                     /locus_tag="P5316_08700"
     CDS             21014..>22244
                     /locus_tag="P5316_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRR85075.1"
                     /translation="MLSNFFLDRPVFAWVIAIIIMVAGGLAIYNLPVSQYPPIAPPSI
                     YIQSFYPGASAETTENSVTQIIEQKMTGLDQMIYLSATSDSAGASRIELTFAPGTDPD
                     LAWAKVQNKLQLAMAGLPEVVQRQGVTVGKATRNWLMIVGLISEDGSMDGHDLRDYAQ
                     SNLEKVLARVPGVGEVENFGSQYAMRIWLNPDRLTEYALTMEDIVMALRAYNVEVSAG
                     QFGGAPAVEGQRLNASIIVQSLLKTPEEFQSIPLRINPDGSIVRINDVGWAELGTDSY
                     EIEGMYNGKPSAAMAIRQVAGANALDTAAAVKAKLEEMSRFFPPGMKVVYPYETTPFV
                     EVAIEEVVKTLFEAIGLVFLVMWLFMGNIRATLIPTIAVPVVLLGTFATLSIFGFSIN
                     MLTMFAMVLAIGLLVDDA"
BASE COUNT         4380 a         6943 c         6501 g         4420 t
ORIGIN      
        1 cagcctgttc agaaatatga gaagcggtag gataagtcgg aaaggtccgc tagaccctta
       61 gactcttagg aagaaccggg catccaagct cctaatagtc tagcagccta aaggactaag
      121 gcggaccgcc tttttgcgga ccgcaaccca agcgctctgc gatcccagaa catgcgcgca
      181 agccgcatag atgtctggcc gtaaggttct aaaagtcatc ctggcttgca agcccgttca
      241 tgtgcgtcag atcgccccat accaggagtc gccaccgcgt tccctggacc tcaaaccgat
      301 tcatgctcgc gttgaacagg ctccatcgcc gcggggcatt gatcggcaac tgcatcgtgt
      361 ggcgaaaggc ggcgctgagc gggccgccat gggtcaccac caccactctg cggcccgggt
      421 accggtcggc gatcgattgc agacctgtga tcacgcgatc gtgacattgt cgcatgcttt
      481 ccccgtcagg aatgacataa tcgacctgac gcgaggcgaa acggtcatat tccaccggaa
      541 atcgccggtt catttcttcg acggtcaaac cctgaaaaat gcccatgttg cgttcgcgaa
      601 gacgggcatc ggggatgacc gtcacgccct ttgccctgcc caatacctcc gccatccgga
      661 tcgcccggcc caagtcgctg ctgtgaatcg cgtcgatgcg ctcttgcacc agtcgtgccg
      721 ccaccgcttc ggcctgacga aagccgcgcg gcgtcaggtt gctgtccgcg tgtccctgca
      781 tgcggccgtc gaggttccac tcggtctcgc cgtggcgaac gatgatcaat tcggtgcttg
      841 gtgtcggtgt catcggcaag gctgatgggg caggtctgcg ttctccttca tcccgaggac
      901 cacggagggc tgcgaccttg ccctaccgtt ctttactgct ccaggctcag ccgccccccc
      961 agtcggctca tacagtccac aaaggtggga aaggtcactt ccatcgcctc tgcggtctga
     1021 atgatggtcc ggccttcgac ggccgacccg gccaccgcca aagccatgac cacgcggtga
     1081 tcatggtggc cgtcgacttc cgctccccgc agacggcttt gctcaatctc caggccgtcc
     1141 ggcctttccc ggatgacggc gcccatcttg gacagttccg cggccatgac ggcgatgcga
     1201 tcggtttcct tgagccgggc ctgcggaacg ttgacgagcg acgtcttgcc aaaagaaaag
     1261 catccgacaa ccgccatcat cggcagggcg tcaggggtag cgttgaggtc aatctcgcaa
     1321 cccttcaatt cacccggtcg aacgcggata ccgtcgggct gaacgtcaat ccgggccccc
     1381 atcaggcgca gatagtcgat cactgcctta tccccttgcg gatcgttcat gtcaagcccg
     1441 cggaccagca catcgttatc gcccagcgcg ccggcagcaa ggaagaaagt cgccgaggag
     1501 aaatccgccg ccacccgccg cgagagcggg cggtactgct ggccacccgg aacgtggaac
     1561 cgcctcagtt catcacgttc gagtgtgata ccgctgtgtt tcagccagtc gagcgtcatc
     1621 tccacataag gcgcctcgtt gagcagaggc acgtcgatct cgctgtcgcc gtcggccagg
     1681 ggacaagcca tcagcaagct ggtcaggtac tggctggaga cggcctcgat gctggtcttt
     1741 cccccgcgga gacggccgcc gaccaccagc ggtgcacagc cattccccaa cgtgctcgtg
     1801 accgaagcac cgaggtcatt gagcgattgt gccagcggcc cgatcggacg ccggcgaatc
     1861 tgatggtcgc ccgtgaacac ggccaggcca ttcgagagca gggccgctga tcccgtggcg
     1921 atgcgcagcg ttgtacctgc gttgcggacg tcaattacgt tggcaggcgc gttcggcctg
     1981 cccgaaaaac catgtactac ccatgactcc ggccgagtgg cgtcgatttc agcgcccagc
     2041 gcccgatagg cgtccaccgc cgccagagta tcggccgaga tcagcggcga ttcgatacgg
     2101 ctctctccat cggcaagggc cgcgaccgta attgcccgta tggtatgcga cttcgagccg
     2161 ggaatgtcga cggctccaga caaccgagac cgcaagcaaa ccagacgcat ctgacctttc
     2221 ctccagcgca acatgcggga ccgccatccg atgtacgacc acgtcttatg tgggagcggc
     2281 gtcttatccg tcggccctgt agggacacta tagccgcaga ggcggcctga gtcactgggc
     2341 tttgctcgta tttcgcaaag cagggccgtg ccgccgtagg cagcaaaggc ctgtcggatg
     2401 atcggtgaga acggcgggct cggtcgcccc gaaggcctcc attcggcttg agctcatggc
     2461 cgaaagcgac actgcccaac caggcaaatg aagaagaagg ggcaccgact tgaggatcgg
     2521 taccccttcg gaatggactc cttcggctcc ggtttaccag ctcatgccat tggttccgga
     2581 gttgctgccg taaatcagat acatctctcc ggtatcgacc ttgcgaagac tcgggttgta
     2641 cgggtacacc gtatcagcct cgggcaggcc caggaggatg tcgtcaaacc cgtcgccgtt
     2701 gacgtcgccc gcgccgctgg cccacgtgat acgagcggcg ttggcatccg tcatgccata
     2761 aggcgttacg aacaccaaac ctggcacggt cgacccgacc tgactcaggt tgatcgtctt
     2821 gttggccaga tgcggcccgc cgaacaacag gtaggccaca ccctgacggc gtaagccgtt
     2881 gatcagtcgg atctcgtcgg gggccacaat caccaagtca tcgataccgt ccgcgttgaa
     2941 gtcgccgacg tcattgatga ccgcgcccgc gtgcccaccg gcctggcgac cgtagatccg
     3001 aacgcccggc aacgtcgggc cgcccacgtg gccgacgttg aacttgttga ctccggcaaa
     3061 atgccgaccg ccgaatacga ttccgatgaa ccccgactcg ggaccgccga taccgtcgct
     3121 gccggcatat tgcgaggcaa aggcgatgtc cgggtgaccg tcctggttca catcgccgat
     3181 cagcgtctgc gattcaccaa accggtcgtt gttgcggtct ccatggattt ccaggcgcgg
     3241 gctgttccaa atatccagat caaattcctc aagattggtc atatcaggat tgtcgaaatc
     3301 gggtcggccg aagacgacat aaacgattcc gttgtcgatc agcccgttac gatcggcacc
     3361 tggggcgccc gccacgatat cctgagaacc gtcgcggttg aaatcaccgg ccgggcccgc
     3421 ataaccgaac agatcgccgg ccatttcgcc ggtgatgact tggcttccag agcgaccggt
     3481 tccatcggtt ccgccgggga taatccgcgt acggaacccg caacccacta acgccgggaa
     3541 cgactggatg tccggagtcg tggagccggg caaccggctg gtgtagtcaa accactcgaa
     3601 ataccagacg cgaccacggt tgcggccgtt cttcggtgag gaaatcacca ggctcggccc
     3661 ccatcgggcg gtcaggcccg gcgtcgagtc ggagatgctg ggcatgctgt tgacggtcag
     3721 cccgaacagg ttgtccggaa tgatcgagta cggccacgtg cgattgaccg aacccgtgag
     3781 ggttcccgga atatcgaggt cgtcgaagta ggctcccctg aatcgtgcac cgttaaagcc
     3841 caaggcctgt cccacatctt ccaacccgta gatgtcgtta ttcagggagt tgttgctccc
     3901 cacaacaatc gcgtagccgc tggcgatggg tgtctggaca aagaggtcct cgtcgttgga
     3961 acacaaatag ggtggtggcg gcggatcagg cggcggttga ggtacgatca agtcttcgtg
     4021 ccagtcgtat gcccccatct cgtcgttatc cggcggctga tccgagtagg ggttgggcat
     4081 atagtcgaaa tagcacgatg ggggactgct gtcgcatgga tcatcgtcca cgcgatcgta
     4141 gaacacctca gtgtagggcg ctccgaaaac gatctcggag agattatcac cggtcacatc
     4201 gcccacgcgg gccacggaca cgaggccctg gctcttcgtt gagtagggca tcgtaaacag
     4261 cgcaccggca taggtggtgg tgatgctgtt cagcgggatg tcccccccga actgctgtcc
     4321 agccatgccc agcaccaggt gggcggaccc catcgcgtcc atgccatagc cccgaccgta
     4381 gcggctgacg atcacaaaat catcaatgcc gtcgccgctc atatcaccga cgcccacacc
     4441 cgtgtagccg gattgtccgt tttcgtcgaa tccatagaac ttcgcgccgc ggatcgtgcg
     4501 gcccacttcg gccaagtcga ccagccccgc gttacccgtg gccgtggagg agaccgtgac
     4561 accgccccag gcataacggc tcaccggcgg gttggtgtgg tcccaggcag tcacgcgaac
     4621 gcgataaggc tcgccgttca cccgcggcgg gagcttcatc gcgttgatgg tgtaagtgcc
     4681 gttcggcggg aagctccctc ccggtgtggt cggattggaa cattcgccaa gggtgccgcc
     4741 gacggtgtcc ttgaagcagc cgagcacgta agctcgcaat tcgccgtcga ccatgaccgg
     4801 gtaaggccca tccagcggat cttggacggc gaaacaggcc tcggccaggt tccgctcggc
     4861 gttcgagccg ctgaagacca gatcgttcaa tgggttctga tccagatcca gcagaatcac
     4921 gatgtccgga agtacctgat tctcaccatc gtcgggatca gacacgcacc aactgatggg
     4981 cacctcgctg ttgtggccga cgctgtagtt cacgctgggc tctcgcgcga cgaccgccgg
     5041 cgacaggttg cctggcacca ttccaggcgg gaagagcatc aatagacccg gtgccacaat
     5101 cagctcgcgt gggttcacgc cgtcggtgat actcgcaatg atgcgatact cgcccggctg
     5161 aaggcccttg gcagtcgtat tcagataata gaaaccttga gaattgtcgg cgttctcgga
     5221 gatattcgat tcgatcagga tctcggtacc gtttccgaag atcttgtcgg ggtcaacgta
     5281 cagagagatt accgcgttgt catcggggtc accatcaagc caggcaatct cgatgatgtc
     5341 gcccacttcc cgcatgacgt cgctcagcgg cttggtgaat tcgaaatagg gaggtgtgtt
     5401 cgtgccgccc ggaatgtcct gcccactgcc cgagccgctg tcggaaccgg tgccgccaac
     5461 ggggtcaccg cccggccata tccagttcat cggcgggcag ccctgcccaa ccagcaaggg
     5521 caccgccgaa agcaacgcta cgagaaccga gaggccgctc cttcggatct ggcgaggttt
     5581 tgtcatcgat gcgatcctta tctatcgtaa gggcttgcca taacgggcat taaggctggc
     5641 agctccatcc gcccgcagac ccctcccgac acacttaccc cgtcacgttc cctggggttg
     5701 ccgtattcta tgtcagccgc aaaagtcagg tcaaggaaac gtaacccgct gggattgcat
     5761 ggccaataaa gagtttgcgg atgacctatc aaagccgaag cagagagaac aaacctagcc
     5821 ccttgtctca gccagccgaa gcagatccct catggccgcc gccgggtcgg gctgaccgaa
     5881 gatagcggac ccggccacca aggtgtcagc cccagcctgg accgctaact gcaccgtcaa
     5941 ggggccgatg cctccatcga tttccagtcg ttggtcgggc ctgagcagct tccgcagttc
     6001 ccggagcttg tccagcgatg actcaatgaa ctgctgaccg ccaaaccccg gccagacgct
     6061 cataaccaac accagatcca ttgctccgat aaccgggcga agagccgatg ccggggtgtc
     6121 ggggttcaag gttatccccg cgcgtgcccc ggttgaccgg atatggtcgg cgatggccat
     6181 cggttccggc gccacttcga tgtgaaaagt cagtaggtcg ctgcctgcct tggcgaacgc
     6241 ctcggcatat cggaccggat cgctgatcat caggtgggta tcgaagaaca gcttggatac
     6301 cggacgcagc ttctcgatca ccggaacgcc gaaactgatg ttcgggacga agtgcccgtc
     6361 catgacgtcg aggtgcagca cctccgcacc ggcagcctcg atcgcttgta tctcgtcctt
     6421 gagccgggca aagtctgccg acagcaggga cggagccatg cggatgcggg gctgggcaag
     6481 tggccagtcg ttattggaag ccatagtact ttgcctcccg gaatagccgc agcatgataa
     6541 aggcccacgg atcgacatcg caacccgtgg gcctcgtcaa tcagaaccag attcgctttc
     6601 tgacttacgc ctcgtcgagc acgtcgacgg ccttgaatgg cttgccctcc atgaactcaa
     6661 ggctggcccc gccgccggtg ctgacgtggc tgaccccgtc cgtcaggccg gcctgttcga
     6721 tggccgccgc gctgtcgccg ccgccgataa ccgtgatcgc ccccttggcc gtggcatcca
     6781 ccaaggcatg ggcaatcgcg taggtgccga cgtcaaacgg tttggtctcg aacacgccca
     6841 tggggccgtt ccaggcaatg gtcttggccc ccatgatgat cttcttgtat tcttcgatgg
     6901 ccttgggtcc aatgtcgaac ccgctgagat catcggggat atcgccttcg actaccttgg
     6961 tggcgatccc gggcttcaac tcggccgcac agaccacgtc caccggcagc ttgagcttcg
     7021 agccgcccaa ctgaagcagc cgcttggcct cttcgacctt ttcggcctcg atcaggctct
     7081 tgccgatctt gcggccctgg gcgagcatga acgtgtgctg catggctccg ccgatcagga
     7141 tggtgtcgca cttcttgagc agggcctcga tgaccccgat cttgtccgag accttcttgc
     7201 cgcccaggat ggccacgaac ggccgctggg ggctgttgag cgcatcgccg aggtattcga
     7261 gctccttctt gaccaggaag ccgaccacct tgggctttcc gcccatcagt tgcggaacgg
     7321 tcaacatgct ggcattgtcg cggtggcagg tgccgaacgc gtcgttcacg tacacatccg
     7381 ccaggtcggc aagctgcttg gcgaagctcc tctttttctc cgccagggcc ggatcttcct
     7441 tggccttctt atctttgatc tgctccgcct cataaaagcg gacattctcc agcagcaaaa
     7501 cgtcaccggg cttcaaggcc gccacagcct tctggacctc agggcccacg caatcgggca
     7561 caaaccggac cggcttgccc agaagcttgc ccaagcgctc ggcacagggc ttgatcgtgt
     7621 acttcgcgtc ggcggcccga tccttgccct ccggccggcc caggtggctc atcagcacca
     7681 ccttgccccc gttggtgatc acgtgttgca tggtcggcat cgccatccga atcctgcggt
     7741 cgtcggtgat gttcccggca tcatcctgcg ggacgttgaa gtccacgcgc atcaagacgg
     7801 tcttgccttt gacgtcaatg tcagcaattg tcttctttgc catgttgtcc atcctcaata
     7861 accaacgttt atcagacccc tcaacgggta atcgtgagcc gagtattgtg caagagtgtg
     7921 ccctgaaagt ccaggcccag gcgactgatc aagcgacctg agaccgggac aacgacgacc
     7981 accaacggct ttggtgtcct gccgtttcgc cgcagctcgc agcaagctga ccacatcacg
     8041 tgcatcccgt tgctccgtca ggtcaaacgc ggacagcctt tgtccctggt gttcgagggt
     8101 cggattcatc gccaattccc gtatgtcgcc gggcgccaat ccctcccggc cgggcggttg
     8161 tccgaaagcg gttgctaccc aggtcggttc tcctgtttcg atcgtgcttg cgaagaaacg
     8221 gataacctcg accatgcctt ccagggggcc ggcagacaag tgaattccgt tgcagttgat
     8281 gctcacctcg cgcatcccga gattgtgtcg attctgtgcc atcagccagc gcatggagtt
     8341 gacagcagcg ggccagggat cggccgcatc cgccggttgt tgtcgcaact ctctgacgga
     8401 cctctctgag gcaatctcga tcgcctagtc cctatccgtt atcgtcaatc cgggcgattg
     8461 atgaggtatt tggcagcgac ggcattgagc gaacgagccg aaggcttgtt cgcgctaaga
     8521 gcccttagtg ccgccaaatc ggattcccag gcaatcatac ccggtgccag cagacacacg
     8581 ctatccggct catacgtatc acaaagccga ccgtccttga agtagtagtg ctccttgcaa
     8641 acttcggtag ggtaaacctg catcagcggc ggaatgtcag agggtgtgaa ccagagtttg
     8701 atctcccgtt cagcatcttc gtggttggct gaagcgtgga tcaggttatc caggcgctcg
     8761 ccgatctcct tgccgttctc atccttgagt gtaacgaccg tgcccagagc ccgcaggcaa
     8821 ccgggttgtt cctccctggc ccgatgggga ttggtggggc cggccatctg ccggatgatc
     8881 cgcagggcgt tgtggccgtg atacacgatg gcgatcactc gacgtttcca gggttcatcg
     8941 ggatagtgag cataaccgcg aatatactgg atgagtgcgg ggaaaaaggg ctttcctgcg
     9001 tgttcggcgt agtgttcagc agccagcatc tcgctgacgt ggatgatttt ggccgcggca
     9061 aaacgcaagc cggtatgcac ctcggacaat tgcgacagaa taaagcccgt aaccgagttc
     9121 ttcaaggcgt ccggcttgat cagaacgagt gtttgctcaa actcttgtgg attcataaga
     9181 atcgtacctc tcgatggtga tattcatacc ttgttaatgg ctctttgaag gcagacacta
     9241 tagcaacaag aagtcacctg gtttcgcccg cgatgttgtc gtcccccttc ctggccgcct
     9301 tccttgggcg gccgccgggc aggggaggca gtcgcccacg gacttcagcg gcttgcacgg
     9361 ccgatcggcg cacggcttga ccgtatactt cgcgtcggcc gtccgatcct tgccctcagg
     9421 ccggcccaag tggctcatca gcaccacctt gccccccgtt ggtgatcacg tgttgcatgg
     9481 tcggcatcgc catccgaatc ctgcggtcgt cggcgatgtt cccggcatca tcctgcggga
     9541 cgttgaagtc cacgggcatc aacacagtct ttcccctgcc gtctaaggcg gtccgccttt
     9601 ttgcgggccg caacccaagc gttctgcgat ccggaaacat gcacgcaaaa tgcgcaccag
     9661 tcgcgacgga agagactaat ccgcaatcgt ttgcttcgct atcttgacac cttcaatcga
     9721 tgcacgaaga ccctcgaagt ccaaacccta caacggcgga tattctggtc caaaacccga
     9781 gacaaatcca gtcggcagga gcgaagatgg cgacagtggc agcggcccgc ctcaggcaag
     9841 aggcgggaat tgctcccgaa gctgcggcag cagctccagt gttccgtggg tgcggcacca
     9901 gcgttcacgg catgccgcat ccaggagttt ctgctgagtg gcaaggatgt tgcgggcgtc
     9961 gttcagcccc ttgctgcgac gctccctttg gtaccagagg agcttggtaa tgattacgtc
    10021 ctcgggcgca ggcacatagg catctcctct catcgcgcga acgacgacag gccggccgaa
    10081 ccgcgattga tcatgttcat cctggctcaa ctcgaacagc ccaatcacaa gcgcatggtc
    10141 cggcgtccac aggacgtgct tgattgtgcc ggtgaccgtc tcgaacgacg tttgcgggtc
    10201 gactttgaat tgacttgcca aaggagcgaa gacctcatcg acagaacgtc cctggagttg
    10261 cacgacaaag tcggcatcct gcctcgcgac ccttcttgga tcatacctca ctcggacggt
    10321 ccagttcgac aggggcgcga actgctcatg gacgcccaag cggtcggcct gcattctgcg
    10381 gtccagttct gtgttggcgg cccgcaatgc actgagatcg tgcgaataga gcaacggcgg
    10441 atctggggct cgaaccgccg atcgcacagg ccggcctcga gaagaccctc aatgacgaaa
    10501 ccgcccaagt acagggcggc ggcatatcgc ttcgatttcc acaggtactc cgaatcatca
    10561 agccattggt cgctcttggt cgtcaaccgg ctcttcttgg tgatctggaa cggtgcgaac
    10621 ggatcgcgtc gttccatggc tttcccgcct catgtggcgc aaggacccga cagcggacct
    10681 cctccatcgc tccactcggg cgacagccgc tccaaaaaaa gggggccgcc tgttcggctg
    10741 tgccccctga gtccgttcgt cagacgctcg tccggtcgcc ttacaacaac ccatccttct
    10801 tcccgatgaa cttcatcagg tcgttcaccc ggcaggaata gcccatctcg ttgtcgtacc
    10861 aggaaatgag cttgaagaag ttggcgttca attcgatgcc ggccgtcgcg tcgtagatgc
    10921 tcgacagcgg gcaaccgatg aagtcgctgc tcaccaccgg ctcttcggtg tagcccagaa
    10981 tgcccttcat gggaccttcc gaggccttct tcatcgcggc gttgatctcg gccatgctcg
    11041 tcgccttttc cgtgcggaca gtcaggtcca cggccgacac gtcggcagtc gggatacgga
    11101 aggccatgcc cgtcagcttg cctttgactt cgggaatgca caagcccaca gccttggcgg
    11161 caccggtcga tgccgggatg gcattctggc cggccgcgcg gccgccgcgg agatccttct
    11221 tgctgggccc gtccaccgtc ggttgcgtcg cggtcatcgc gtggatcgtg gtcatcagac
    11281 cctcaacaat gccgaagttg tccaagacga ccttggtgat cggcgccagg cagttggttg
    11341 tgcaactcgc gttcgacacg atggtatccg tcgcggggtt gaacctctgc tcgttcacac
    11401 ccatgacgaa cgtcgggata tccttatcct tggccggagc ggagatgatc acccgtttgg
    11461 cccctgcggt caggtgcttg ccggcgccag ccctgtcggt gaatttcccc gtcgactcga
    11521 ccacgtaatc gaccttgagg tctttccacg gtaactgcgc cgggtccagg attgatacgc
    11581 atttgacctt ccggccggcc accacgatgt tgtcaccttc ctgcctgacc tccacctttg
    11641 cccggccgtg gaccgagtcg tatttcagca gatatgccag attctcggcc ggaactacgt
    11701 cgttcaccgc cacgaactcg atatccctgt cttccatcgc cgcccgaagc acgagccggc
    11761 cgatacgccc gaagccattg atcgctacac gaatcgccat tgtcttttct cctgatgctg
    11821 taacctgtct ccgtcaattg gggggtatac cttgctggat ttccaactgc ggagtcgaga
    11881 cgatacggct tttgggcggt caggtcaagg ctcggcgccg cggctgcttg ctcgctcgcg
    11941 aatgtccgcg acgggcagga tggctcgttc agagccgcaa gcccgattcg ccgagccgac
    12001 ggcaacggag atgcccccca tctggtacgg cgccgcaggg gttaggctca gcgcgagggc
    12061 acgcattcct atggacgacg cagcttggag tcgctggccg aggtggttgg aaccctgtcc
    12121 gaacgatcac cctggaaacg aatctgtaca acttcctcga cgttccatgc ttccttcata
    12181 ctcaactggt ggcgaaaaag cgtcagccgg gagactgagc ttgtgctgga acgccccggc
    12241 acattcaact ccttaacgaa caaatccttg cgcaagacaa gacggcgcac cggtcgctca
    12301 aaagccaagc cgctgcgccg ttcgcggcga cagatgagaa aagaccgtcg atgcacgaag
    12361 cccctctcgg tcgatatgta aggtacgggg gcgaggccaa cctggaacac ggttgcccaa
    12421 ggcggactgc tttcttgcaa gcggctgccg aaaagcctta cggctcagca agatgcgggc
    12481 agaacccggg actcgctggc ccggaggttt ccggggtcac cgcaaggttg gaaacgccca
    12541 cgagggcgtc atagaatctg gattggaaga atcgttcgtc ctacctgaca aaggagcggg
    12601 ccatggtagt gaagaatggc aaaacggtaa ccttcattta ccggcctgag caccctgcgt
    12661 gtcgcgacgt ggcgctggcc ggttccttta acgactggaa gccggacaaa ggcaagatga
    12721 ctcgccagaa ggatggtacg tttcgcaaac ggatcacgct tcgacccggc gagcatcgct
    12781 acaagttcgt ggtcgatagc cagtgggtcg aggaccccca agctgaaaag cagactacaa
    12841 acgagtttgg cacccgggac tcggtcgttg tggtgtagag ccgggccggt cagatagagc
    12901 ttgttgcttc gagcgaattg gcgggcgcga tgcctgaagg gtgatccccg tatcctgcca
    12961 aagcggatca ttgtaatctc aattatcaca atgggttgtg tcaactgacg gcgtgtgccc
    13021 cggtacatct tttggcggtc agtcgtagat gccgaattcg tgggctgcca gatacatcgc
    13081 ccggaggttt tccagcgact tgacggccaa gggcaccatg aacggcggcg aagtaggcag
    13141 caagacaaaa gccccgtcgc cggccgctgt ctccactgca tcccgtactt cacggcgaac
    13201 atcctcgggc atgccggttt caagagtgtg ggcttggatt gcccccatca aacacatccg
    13261 ccgcccgatc cgctccttca cttccgccag cgtcacgtcc gcggtggaca tagggagcgt
    13321 ttcgattggc tccaaggcat cggccccgat gtcggcgatc atgtccagaa gatcgcgcag
    13381 acgaccatga caatggatca cgcagaagtt gcccgtggcg tggatggctt cggtcgcctc
    13441 gcggtccctg cctacgacat accgggcgaa gtaaaccgcc ggatccatca gcggcgcgcc
    13501 ggcatattcc ggtccccaaa gccggaacgc ggcattcctc accaacggcc cgatcgcctt
    13561 gattgtccga agaacctgcc cttgagcttt gtcaagcagg gctcggatcg ggccgtcgtc
    13621 ggtgtagcac cgcagcacaa agtcttcaaa gtcgaacaaa cccacgacgt ggccaagggc
    13681 atcgcccacg ctgaaacaga gaatgccgtc gtctccgacc cgagcctgca ggtcggcgat
    13741 tcgttgggcg ttgacttcgg ccgggggatc cggcatcgag agcaatcgtt cgatatcctc
    13801 gtccgatttg atcaggggtt cgacttgcca attggtcatc aggccgggat ctctgcgaga
    13861 gacggcccgc agcctgcctt tcggggtgtt gatttcggtc gtcatcacaa ccgagccgtc
    13921 gggatgaatt tcttcctgac cgtgaacgac gttcggatct ccaaggaaga ccggacaatc
    13981 gatcggggca gtgacaaaac tgtcgccgta ccgactctcc agctcgatca gcggggcgta
    14041 ggatggctca tggttgtacc acgcgtcgtc atgtttcgag tagccataga tcgtcaccgg
    14101 cactcggtcc ggtcgccctc gacgaaggac ggtcaataat cgctctcttg aggtcatggt
    14161 tcgctcgcat cggccgacgg tcacaccagc ggcttgccga tcatgtcggc gggtaccggg
    14221 tagcccagca tcttcaggca tgtcggcgcc acgtcgtaga gcgtagcgtc gtcgcaacga
    14281 ccccgccctt ccagggacgg atgcgacaga atgtacatgc cctccagagc gtgattcgcg
    14341 tcatcaggtc ccgtgtcatt ctcgaaagtg tataacgagt cgttgcccac ccgcccgacg
    14401 cttcgccagt tcagattgcc gaaaatcacg atcaggtccg gcggtacgcc gttgaccttc
    14461 ttgtagacat cctgtggctt gaaggcctgc gtgcccatca cgtggccatt gtggtctcgc
    14521 aacgcctgca acttgcagat cagctcgtcg cgtaccgcct cgtactggtc gcggggtatg
    14581 atgccttgcg gttcccgccc ctcaacgttc aggaaacacc ggccataata tccgccctcg
    14641 ccccaggcct tggtccgcga ccaatcgacg ttggcaatgt caaacttggt cggcttggag
    14701 ggaggctcct tcaacgccag atagccttcg cgaataagcc agtcgttgaa gcagaagccg
    14761 ccgatcatgc acttcgaacc atgatcgctc accacccata ccgccgtggt ttcgaaatca
    14821 atgacttcca gcaactctcc caccagccgg tcaagccgga catagtagtc gcgaatcgca
    14881 gactcgaacg gccctcccgg tacatatcga tggtgggttg agtccatgaa ctgccagaac
    14941 ccgtggtgga tccggtcgac gcccatctcg accatccaga acaggtccca gggccgcgtg
    15001 gtgatcagat gacgagccag cttgaaccgc ttgtcggtca tctcgttgat ctggtccagg
    15061 agccactgct tgttgtcggt ccggaagtct ttaacgtcaa taatgtaatc cccgaccacc
    15121 cgcgaaatct cgtccttgag ggtcttcgga taggtgtagt cggaattggt gtccggggcg
    15181 aggaaaccgg tcaccatgag gcctttgggc ggccgcgcga tggggaatgt acccggcact
    15241 cccaggatga tgttttcgcg cccgcgggcg ctcaggatat cccacaagcg aggctcgcga
    15301 accgccaagg aagttgcgat cgagagcttc tcatacgagt aatcagcgcg gttgcggaac
    15361 ccgtagatgc ccaattggcc cggatccttg ctggccgcca tacaagacca ggccgggacg
    15421 gttatcgggg gagtgcaact tagcaggttc ccatactgtc ctgtctcaca cagccggcgg
    15481 agattcggca gatccggcaa ccagcggttg aagaccagag aaggctcccc gcagtccaga
    15541 ccaatgatcg ctattctctt gatgtcaggc actgttccct ctcttcatgc cccgggcgga
    15601 caaaagccga acggctcctt gtaacgtcgt cacgatcatc tgacaagcgc ggggacatct
    15661 cacccttcag ggcggcccgg atcggcggga aaacgagtgg tacgaccccc tcgccaagcg
    15721 accgttcgtc acgtacaatg ttttggccgg cgcaaatgag acgccccgct gaatcctgcg
    15781 gacaaactgc atggccgtcc tgaccacgct ctatgagcct atcgccaagg acctggagaa
    15841 agtctgccag atctttgatg atgaactgtt ttccgacctc ccgctgatca acgatctttg
    15901 tgctcatgtc cggcagtacc gcggcaagct tcttcgcccg gctctgctgc tgttgtccgg
    15961 caaagcctgc ggcaagacca ccgacgtaca cctgaccctc gcggccgtcg tcgaaatggt
    16021 gcacatggcc acactggttc acgacgacgt gctcgacgat gccgatctgc gccgacgttg
    16081 ccctacgatc aaccgtttgg aaggtaacga ggccgccgtg ctgctcggcg actacctcat
    16141 cagccacgct ttccacctgt gcagttctct cggctcccaa cacgcctcgc gcgtcatcgc
    16201 cgcgaccacg aacatcgtct gcgaaggcga actgcaacag gtccaccatc gtggtgactt
    16261 caatctaggc ctcgacgatt acctgcagat cattacccgc aagacggctt ctttaaccgg
    16321 tgcgtgctgc cggttgggag cccacttcgc gggagccgga actgaaaccg ccgccaacct
    16381 tgagcggtac ggactggcgg tcggcatcgc cttccagatc accgacgaca tcctcgacat
    16441 catcggctcc gagaaacgga tgggcaagac cctcggtcgc gatcttcaga agagcaagct
    16501 cactctgccc gttatccatt gtctcacgcg ggccgacgat accctccgaa aagagttgca
    16561 tctgctcctg gcagggtcta atcctgaccg caaccgcctg cgaaacctct tggagacgac
    16621 tgacagcatc aactattccc ttcatgccgc cagggactat atcgagtcgg ccatcgcccg
    16681 cctggatgac ctgccgccat cgcaggcccg gcacagcctc tcccgactgg ccaggctgat
    16741 catttcgcga gagcaataag ccggatcact tgcacccttc ctgaccggcg aaagagatcg
    16801 ccatggatcg cattcgagtg cgcgacggca ttggcggggg aaaccgccac ggctcgttcg
    16861 gacgattttt cagaaccgcg accccggttt gccgaagcga tcgtaataaa cccgtctcct
    16921 tggacatgct tttcttgtgg catggcgtgc cctccaccga ggtgccgtga tgtcggttcc
    16981 gggtcccttg cccattgcca ggcttcgacg cgacccatca attggactct cgtcgccccg
    17041 gttcggcgcc gccgggcgca tccacggcgt ttcctcccaa ggaccgtcgc aaaaattcac
    17101 agatgctgat gattgactgg tcaggaaaat aaactcgatg ccatcatctg cttccaggta
    17161 cagtggtttc tcgagtggct cagcctgtca caacggatcc gcgactgcct tgccatcagc
    17221 aaaagctggc agcccaaatg tatcgtaagt tgtttacaga taagttatta gagcagcctg
    17281 aatcggtgcc atttcgttgg atccatagga accttgcatg tgtaccgtct ggacggtaca
    17341 gtatctcatt atgaatgaaa cgcccgaaaa cggagcttct gtttccgttc gtgaccggct
    17401 tctggatgcc gcagaggcag tagttgcccg ggaaggggtc tcacgcctga cgctcgaggc
    17461 cgtggctcat gaggccggcg tcagcaaggg agggctgctc taccacttcc ccgcgaaggc
    17521 ggcgctgata accgctattg tcgagagact cgcgattcgg tgcgagtcga ggcaggccga
    17581 ggccgttgcc agcgatcccc aggcctgcgg ggccttcatt cgtgcctacc tcaccgtccg
    17641 gacccaccca cccgacccac aaagggagtc gatccacacg gccgtcttgg cggcgattgg
    17701 caccaatccg gattacctag acccgcttcg ccggcgcatc gccgagtggc agaaaaggtt
    17761 ggagtcggac ggtatcgacc aagtgacggc aaccatcgtg aggcttgcgg ttgacgggtt
    17821 ggcaatcggc cgactcctga aagttcctat acccgaaggc gaactgcggg agcgggtgat
    17881 tgagaggctg ctttccatga ctcatccgca agcggaggaa aagcgctcgt gattgcggac
    17941 ggctcacctg cctgttccgg atgcgtggta ttgcagccga ggagttgcgt atgacaggac
    18001 atcaaccggt tgttgcgtgt tctttcatgg tgatcgtctg cctgtgttgc ctcccctcag
    18061 gttgtagcgt cgggcccggc tatcgcgccc cgacgcctga gctgccggag gcgtggcatc
    18121 aaaccacgac gggcgggccc gctgaggaaa acgccgacct gcaggagtgg tggcacgtgt
    18181 tcgaggatga ccttctctct gacctgatca aacgtgccgc tgagggaaac cttgacctgc
    18241 gtctggcggt tcttcggatt cgggaggccc gtgcgctgcg aaacatcgcc gccggaggac
    18301 tcctgccctc gcttgccggc caaggcggtt ggcaacgttc gagggcgagt gcgaacggtc
    18361 ttatgggcgg cgccgtgcag gctcccggca agggaacaca attcgccaac accatggcac
    18421 gcggcatcgc gagcagtgct ctcagcgagg gcttgggcac tgccttcccg ggcgcgcccg
    18481 ccgtgacaaa ctcgttggcc ggcgggctga tgggcttgct tcccaccccc acaagactgg
    18541 caaaaacgga ggcgatggac ctctacacca caggcttcga tgcctcttgg gaaatcgacg
    18601 tctttggcgg cattcggcgt aacctcgaat cggcagatgc tgttctggcc gcggccgttg
    18661 aggactatcg cggcgtgctg gtcagcctcc tggccgaggt ggctgcgacc tacgtcgatg
    18721 ttcgctcgct tcagagccag atcgaagcga cccaacgcaa tatcaagctt cagaaggaaa
    18781 ccgtggcctt gacacaggtg aagttcgatt ccgatcttgt gtccgagctg gatgtccgcc
    18841 aggcggagac caatctggcg accacggagt ccgaattgcc gctgatcgag gccgctttgt
    18901 cggcagcaat ctatcgcctg agcgttttgg tcggccggga accggcggcg ctgtacgacg
    18961 agttgtcgca ggtacgcgat attcccaaac cgccggcgga aaccctcatc ggcgttccgg
    19021 tggatcttct tcgacgccgt cccgatattc gcgcggccga gcggcgtctg gccgcgcaaa
    19081 ccgcccaaat cggcgtagcc gccgccgacc tttatccgag attcaccctc tcgggcactt
    19141 tcgggttcca ggcgacggac atcaatcaca tgctcgactc ccggagcgtc acgtatggat
    19201 tcggtccggc attgcggtgg aacctcttcg acggactgcg gaatctcaac cggatcgccg
    19261 ctcaacaggc ggtgacccat caggcgtatg ttttctacga gcaaacgctc ctgcaggccc
    19321 tgcaggacgt cgagactgcg ctggtcgctt acacgcgcga gcaggcccgc tacacggcgc
    19381 tggtgcgggc aacggaggcc gtccgccgct cggcccaact cgccgagaaa cgttaccagg
    19441 atggcctgac cgatttccag aacgtactga ttgcccagcg ttcgctggtg aatctggaga
    19501 ctgcccttgc gcagagccga gggcaagtcg ccgtcaatct cattgctttg tacaaggcgc
    19561 tcggcggcgg ttggtcgacg gacgccatgc cacaggagca attcctggat gacagctcgg
    19621 gcgtgcttgc cgaccctatg ggcttcttct tctccggcgg caaggggacc ctgccgtggg
    19681 aaaaccatct tgacgagcaa cccgagacgg gaccgaattc gaccaacccg gagtaaccct
    19741 gagttgaata cggccacaga gaaaacgctg cattcctcaa atgaggatgt gaggtgctga
    19801 atgctgataa cggattcttt cacacgaata agcctcttgg gcttcctcgc cgcaggcttg
    19861 aacgtgctgc gctgcaatac acaagaggca ccgcctcggc cgcaaggccc gcccgaagtc
    19921 gcggttgcta ccatgtcccc ggaaccgatc gagttgatta ccgagctgcc gggccgcgta
    19981 tggccttact tggtggccga ggtccgtccc caagtgagcg gcattatcca gaagcgacta
    20041 ttcgaggagg gaagcgacgt ccgagcaggg gacgtgttgt accagatcga cgcggctcct
    20101 tacgaggcgg cttacagccg ggccaaggcc gcactcgcgg cggccgaggc aaatctccct
    20161 gccgtcaagg ctcgggtgga acgctacaag aacgctttgg ccgaacgagc ggtcagccgg
    20221 caggattatg acgaagcggt tgccgccttg gaacaggctc gggccacgat cgagctgcgc
    20281 agggcggagg tcgagagcgc ccgcatcgat ttgtcgtata cgccgattaa agcgccgatt
    20341 tcggggcgca tcggcaaatc gaacattacc gttggcgcac tggtcacggc ccatcagccg
    20401 actccgctcg cgacgatcca gcagatcgac cccatttacg tcgacgtaac tcagtccacc
    20461 gctgaactcc ttcgtctcaa gcaaggcatg gaaaccggca gactcagcaa ggatgacgag
    20521 aatcaaagaa gagtgcgcct gcgattggag gacggaaccc cgtatccact cgatggcacg
    20581 ctgcaattcc gtgacgtgac cgtcgatcca tcgaccggct ccttcattct tcgcatcgtg
    20641 ttccccaatc cggagcatct gctcctgccg ggcatgttcg ttcgggctct cgtcagggag
    20701 gggattgttc agaacgcgat cctcgtcccg cagcaagccg tctcccgtga tcacaagggg
    20761 aatcccgtgg cgctgatcgt cgatgcggaa ggcaaggttc agcagaagac cctcgcggcc
    20821 gaccgggcca tcggcaataa gtggctggtc tcctccggcc tcgagcccgg cgatcgcgtg
    20881 atcgttgaag gcctccagaa agtgcggccc ggcatgcccg tcaaaaccgt gccgttcgat
    20941 gccggtcaga acgatggttt ggcggaacaa ggcgtccagt cggcccccag cgcgaattaa
    21001 gcggagcacc acgatgttgt ccaacttctt tctcgatcgc cccgtcttcg cttgggtcat
    21061 cgccatcatc atcatggtgg cgggcggttt ggcgatttat aacctgcccg tgtcccaata
    21121 tccaccgatc gcgccaccct cgatctacat tcaatccttc tatcccggag cctcggcgga
    21181 gaccacggag aacagtgtga cccagatcat cgagcagaag atgaccgggc tcgaccagat
    21241 gatctacctc tcggccacga gcgactcggc gggagcctcg cgcatcgagc tgaccttcgc
    21301 tccgggaacc gacccggatc tggcgtgggc caaggtgcag aataagctcc aacttgccat
    21361 ggccggcctg cccgaggtgg ttcagcggca aggcgtcacg gtcggcaagg ccacccgcaa
    21421 ctggctgatg atcgtcggtc tgatctcgga agacggcagc atggacggtc acgacttgag
    21481 agactacgcc cagtccaacc tggaaaaggt gctggcccgc gtgccgggcg tgggcgaggt
    21541 cgagaacttc ggatctcagt acgcgatgcg catatggctg aaccccgatc gcctgaccga
    21601 gtacgccctg acgatggagg acatcgtcat ggccctccga gcctacaacg tggaggtctc
    21661 cgcaggccag ttcggcgggg ctcctgcggt tgaaggacag cgtttgaacg cctccattat
    21721 cgttcagagc ctgctcaaaa cgcccgaaga gttccaatcc atccccctcc gcattaatcc
    21781 cgacggttcg atcgtgcgga tcaacgacgt gggatgggcc gagctgggca ccgactcgta
    21841 tgagatcgag ggcatgtaca acggcaaacc ctccgccgcc atggcgatcc gtcaagtggc
    21901 tggggccaat gccctggaca ccgcggctgc ggtcaaggcc aagctggaag aaatgagccg
    21961 gtttttccct ccgggcatga aggtggtcta tccgtacgaa accacgccgt ttgtggaggt
    22021 ggctatcgag gaggtggtca agacgctttt cgaggccatc gggcttgtgt tcctggtcat
    22081 gtggctgttc atgggcaaca tccgggccac gctgattccg accatcgcgg taccggtagt
    22141 gcttctggga acctttgcaa cgctgagcat cttcggtttc tccatcaata tgctgaccat
    22201 gttcgccatg gtgctggcca tcgggctgct ggtggacgac gcca
//
LOCUS       DAOTPW010000075        22230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2408_length_22230_cov_5.157971, whole genome shotgun sequence.
ACCESSION   DAOTPW010000075 DAOTPW010000000
VERSION     DAOTPW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22230
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2408_length_22230_cov_5.157971"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1207
                     /locus_tag="P5316_08705"
     CDS             305..1207
                     /locus_tag="P5316_08705"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR85076.1"
                     /translation="MSEAGQCDSSAEHRLVGWAVYHRPAVVLSLLCALMATGCTIPLR
                     STSELRQPARWTKGYTIILPGIEGRSNLNESIAFGLNKGGVPGAIEIYDWTVGGTFTW
                     ILNLRWSEHNRKQASRIAGKIITYQDTYPGRPVCLIGHSGGGGVAVLTLEALPAEHKI
                     DHAILLAPALAPDHDLRRALSRTRSGIWHYYSKYDVGFLQAGTAIFGTVDGRHSRAAG
                     AVGFVKPWGMDKAGHELYDRLLHQQEFTPQMAEAGHYGLHTTWARESFVSRWLAPIVK
                     TAYTQVPEYASDSGASAAQPSAPK"
     gene            1383..3053
                     /locus_tag="P5316_08710"
     CDS             1383..3053
                     /locus_tag="P5316_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR85077.1"
                     /translation="MGHADVTVGKVLLFQPEGSVVAGLSELFDRFGQVRVESTIQGAL
                     RALDEEQFGLFVSTSPEFLAADRLAAHRQLGAVLDVIGQGVCIINLSGEVTWCNARAE
                     KLPEEVLERIRKHCVTAYGSPEGRAIVGRSRSLSLVVDNDRYYDASITPIEDRERQVT
                     RLVVAVSDVTRARRLQQKMDAIDNAGRELVRLDAQELAKMDADQRLALLERKIIRSTR
                     ELLEFNNFAIRLLDERTNKLELVLSAGLLPEGEEIDIYASTENNGISGYVAATGRSYI
                     CPDVQKDPRYIVGISNAKSSLTVPLWLHDKVVGVFNIESDTAGAFSEEDRQFAEIFGR
                     YVAIALHILDLLVVERHATTGRLADNVSSEIAGPLSDILSDATTLMEDYIGHDDLRHR
                     LQCIVDNVDRIRTLVRQVVRPTSGILGAKPTQPAKDPILSGKSVLLVDDEEIIRQTVC
                     DVLSKYGCEVEVARDGVRALAMIHARSYDLVITDIRMPGKSGYEIFAAVKEAHPGCPV
                     IFMTGFGYDPNHSIIRASKEGLSAVLYKPFKVDQLLTDVRAAISPGKR"
     gene            complement(3288..3632)
                     /locus_tag="P5316_08715"
     CDS             complement(3288..3632)
                     /locus_tag="P5316_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRR85078.1"
                     /translation="MHKLLGAMDQGTLSAATSELTLAEVLVKPKQDKNAALVNEYCDF
                     LEPSAALTLAPITQAILIEAAEIRAATGLKLPDAIHVATALAMQCDTLLTNDRAIRAT
                     RGISVRLLSDLE"
     gene            complement(4076..4996)
                     /locus_tag="P5316_08720"
     CDS             complement(4076..4996)
                     /locus_tag="P5316_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR85079.1"
                     /translation="MKACSRREVLTAAAGMSASVFTAHLARAADAAAGDSTVRPQSPK
                     RFKISLAGYSFRQFLDQPGQPGKMSLFDLVDLCVKLGIDAIEPTAYYFLKTDDEYLYA
                     LKRKIFLAGLEISASPMRNNFCLPPGPELDKELESVRNWVDVCVKLGAPAIRIFAGKA
                     MPGAGRDKDFERAVAGMKKACEYAGSKGIFLAIENHGYLTETADAVIKIIEAVKSDWL
                     GINLDTGNFVEKPYENIAKAAPHAIICQFKTEVVKTPGTKEREPADFARIFKIIREAG
                     YRGYVTLEYEGADPHKEVPIYIAKMQELAG"
     gene            5277..7175
                     /gene="pabB"
                     /locus_tag="P5316_08725"
     CDS             5277..7175
                     /gene="pabB"
                     /locus_tag="P5316_08725"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRR85080.1"
                     /translation="MPADESARGVCQTMKARRIIPTYSVLLRTDGAEGPVAWRLFHSP
                     RFMICATAVEDVVPCLHELERAVAGGCHAAGFIAYEAAPAFDKALVTHAPTTVPLMWF
                     GLYDPPETVAELPKSDSDELPACHWESQMSEAAYRSAVEKIKHYIAEGHTYQVNLTHR
                     LRSRLQIEPYGFFRSLYRAQPVPHAAYLDLGRYAICSLSPELFFRLEGRRIISRPMKG
                     TAGRGRWWEEDERRAAWLADSPKNRAENAMIVDMIRNDLGRIAEKGSVEVEEVFKVEQ
                     YRTLFQMTSTVAARTNAPVSEILRALFPCASVTGAPKVRTMQIIRELEVGPRGVYTGC
                     IGYVSPSRKALFNVAIRTAVVDAEDGRIEYGTGGGITWDSSATDESMECRLKVSVLER
                     PDPEFCLLETLLWDGRKYRLLDGHLRRLKRSADYFGYPIDLRAAGRTLEEASKPYALR
                     LARVRLLVDETGKATCESHLLERCRPRPTCRLAMACRPVDSSDRFLYHKTTHRTVYET
                     ARAASPGFDDVLLWNERSEITESTIANVVLRFGDRLVTPPISAGLLPGVFRERLLERR
                     VIREESMPIEALASATAVYLVNSVRGWVRASLHDPHGILRSVGFQRDTMPSNEEEASS
                     EPCGLDPR"
     gene            7129..8349
                     /locus_tag="P5316_08730"
     CDS             7129..8349
                     /locus_tag="P5316_08730"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85081.1"
                     /translation="MKKKHRQSRVVWIRDDRPVTPEEVAWLKTPAAQAVCAAMMGDEP
                     ADTPAAIERWRQRLEPDQVAAAWHQTLLRRAGRAKFSRADEMLFDRVGLEQASDEVVA
                     AHKAGRFSDCGRIADLCCGIGGDTLALATQAEVTAVDWSRSRVLMAEHNAAVYGGRIV
                     GKVGDVLIHRPDADAIHIDPDRRPEGARRHDPVLASPDLEELRRLVERYRHAAIKLSP
                     GADLSRLPFDAEIELISHMGACKQAVIWTGRFKQVYRRATVLPAGDSVFSSEVDPPAW
                     PEPRELRPGCFLYEPDAAVIRAGLVGAVARQLELAPVDRQIAYLSGDRLLSSAFVTPF
                     QVIDVTDFSARKARTWLAAHDVGRVHIKTRGFAARPEELLHRLRLRGRHEAVLLLTRV
                     AGRPTAVLARRLRR"
     gene            8437..10134
                     /gene="glgP"
                     /locus_tag="P5316_08735"
     CDS             8437..10134
                     /gene="glgP"
                     /locus_tag="P5316_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRR85082.1"
                     /translation="MMVDERCIAYFSMEIGLDALMPTYSGGLGVLAGDTIRSAADLKV
                     PMVAVSLLHRKGYFYQRLEASGWQLEEPVEWNVDDFVTEENVRASVTIGGRPVRLRAW
                     RRDVTGVSGYVVPVYFLDSDLPENAEQDRMLTHWLYGGDDNYRLCQEIILGIGGVRML
                     RSLGYRQIDRFHMNEGHASLCTLELLDERRRAGNRDAIVQEDVEAVRRMCIFTTHTPV
                     PAGHDKFPVDLVAEVLGDHPALTMRNLICCDGVLNMTYLALNLSHYVNGVAKKHGEIS
                     RHMFASYVIDSITNGVHAATWASEPFQALFDRHIPGWREDNFSLRYALSIPHKEVWNA
                     HMAAKRDLIDYVNRETNTGMDVDFLTIGFARRAAAYKRADLLLRDPDRLRKIARDVGP
                     FQIVYAGKSHPRDEEGKKMIQRIYQARKSLRDAVHIAYLPNYDMTLGKLVTSGVDLWL
                     NTPQRPMEASGTSGMKAALNGVPSLSVLDGWWIEGCIEGTTGWAIGRDGRTPETSADN
                     QADAASLYEKLEQVVAPLFYRDRDRYLDVMVHAIALNGSFFNTQRMMQQYVLKAYFR"
     gene            complement(10274..10609)
                     /locus_tag="P5316_08740"
     CDS             complement(10274..10609)
                     /locus_tag="P5316_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRR85083.1"
                     /translation="MKKKFKCVCGYIHDGDGPPTVCPKCGAPAEKFTALDDHAANLVE
                     RSRRTNMLHAHVIDLARQIERACKDGIQDNLDPGCVDVFQKSLEASYVMMKLSMTEIQ
                     SHMNKGKWG"
     gene            complement(10757..11416)
                     /locus_tag="P5316_08745"
     CDS             complement(10757..11416)
                     /locus_tag="P5316_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85084.1"
                     /translation="MNSRIFSTDRYQGIARRTKEFLNGHEDFLSPSTARSPRAFGDAL
                     EGILGDNFRVILGEDCAEYSANFARRAMADIAFKDNDGLYYVVDVKTHREDTKFNMPN
                     LTSVERLTRFYEDDSNYFVLLLVRYRLEGTHAVISEVTFVPIEFLDWECLTIGALGWG
                     QIQIANANHISLKPHYSRKSWMLELCDIMLDFYPREIGKIETRIEYFQRVRQHWEAKT
                     E"
     gene            complement(11426..12439)
                     /locus_tag="P5316_08750"
     CDS             complement(11426..12439)
                     /locus_tag="P5316_08750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008310221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRR85085.1"
                     /translation="MNEKPRAPRNRTLVLSDADRRRCSQSLLRLDSPASLPDVLDRTI
                     CQDAFEVLPLLPRAAFDLIVADPPYNLTKSFNGNTFKQTDLDKYEAWLSSWLPGLRCL
                     LKPTGSIYVCGDWRSSTAIHRVLLRHYKVRNRISWEREKGRGAKTNWKNNTEDIWFAT
                     VSDDYVFNIDDVKLKRRVIAPYTDDLGRPKDWQQTDQGRYRITHPSNIWTDLTVPFWS
                     MPENTDHPTQKPEKLLARIILASSNVGDVVLDPFLGSGTTSVVAKKLQRHFVGIEIDR
                     EFCCLAEKRLHLAEADPGIQGYTDGVFWERNSFSDQNNGRGTPRTPAAGPPSLFDPMP
                     TTK"
     gene            complement(12565..14586)
                     /locus_tag="P5316_08755"
     CDS             complement(12565..14586)
                     /locus_tag="P5316_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85086.1"
                     /translation="MFTHQRITVTTALLSVSLATVMPSLASHSFSGKYFAGSGDVEYL
                     EMLDVARRMFDPDPEFQNMAMLYTPAWNGLVEGPTWGAWWIQNSYGTTYCALPFYIEP
                     MTTFLQNSQDLWFDQMGDGKTVRPFQMGSGPKFDWVPPDGCLCDAASPGWFVAKQGDG
                     RVDIHDWGMEFTAAGVLMQAELLLISRDPEAIAKYLPKLERSANFIESRRDPKNNLFL
                     AGPAGNLLAPSYAGWKKPDGTYDKAYLAGLSITYIAALDRLIELEKLAAFSDKAKLYA
                     ERRELAREGLPRLTTDEGYFIKSLDPDGTRHGVFGASRHGYFETSPNHDAIAFRVVDK
                     PQAERIYAKIASIPGLRPHAFVIPNFPSLDDMYEQPKGLWEFGRWVNGGHWSTCEARM
                     IMAYYRLGRHDDILRSMRQLMKFARTFRMDNPLVDFGDNVYQPGEPINICYDTFGPAA
                     AMVRGLFEYLYTAEGLRLVPHIPPGITFLEQKFPIRFGKSRLFLSISGRGPVTGVRLD
                     GRAYDNFDAESVMLPYGAVQETSFIHIELGNVSRPPGPGPAEIGSPWPAISVDDPELG
                     KLAPRLARLQSFHDRVLREQVPADYEAAHARLAAECAAVILERRKLKEAGKLAPLPDK
                     SQAAAEKSYLDTATRLLEGLEKTLASYEKSQEALKQRIEQVWRETKAAP"
     gene            complement(14898..15938)
                     /locus_tag="P5316_08760"
     CDS             complement(14898..15938)
                     /locus_tag="P5316_08760"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRR85087.1"
                     /translation="MATHERLHPKEPERLSLARIPTPVTELKNTARQIGISRLLVKRD
                     DQTGFELSGNKIRKLEYILADAIASGANTLVTHGGFQSNHCRATAAAGASLGLRVRLL
                     LRSADPHPADDGNLFLDRLFGADVSIHHPDEYNGRRSEMIEAAMEAERRAGRRPYFFP
                     VGASIPLGCWGYIRCVAELVEQIGRDTPLAIFCAVSSVGTYTGLSIGRALLGCRNWRI
                     RGIPVSDNVEILSEDIRKLERQTNERFDLGLREADTPIDLIDGFIGEGYAIPYPEEVE
                     TIRSVARSEGMLLDPVYTGKAMTGMLATIRNGGLRPDETPVFIHTGGAFGLMARRDLF
                     SQPSRRDRDPSQ"
     gene            complement(15939..18164)
                     /locus_tag="P5316_08765"
     CDS             complement(15939..18164)
                     /locus_tag="P5316_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRR85088.1"
                     /translation="MMMHVRHCSRVFKWMLVAAALTPAAVLASAVAADGTVQDFCFLH
                     ISDMHVSPVPLGTGKEDAARLSAAGIAWVCDEAAKPQVLGPLNMRTPPPSFLVATGDI
                     TEYGVINETWGVVEDLFKPLSIPWYITPGNHDNTWTAIQRIMRQRHCGDHYSFDRFGC
                     HFAFINTATPQEPVPTIEQRTLTWLADDLKKVSKTTPVFVLCHHPLSSTEFAKPYEQL
                     RLIELLGSRNVVLLLMGHGHGARHERWGTIDSVMGGSTYGPNTGYGIISVMNGVLRST
                     YRFHDSSKPMALLIEKPIAPADKAQLEFQLPPRKGDNRPMIRTAGVPVRVRISGGQPQ
                     SVTACINGDDQGGVALKGDNQAGTFSGSLPAGFLPPGVHFVRVMADMGPYQLDRACEF
                     TYKGKSTGPVGRVAQLAAGVKATPLAADQEAVVCTTTGEVVRVSFKEPVPTITRLLDV
                     GSEILHPPAQAGDVLYIGAAEKGVYAISAKGKPVWQWKPPAPGNVVYGTPAVDQARVY
                     VGDLEGFVHAIDRKSGQSLWSKRHATFSIEQSLLLRDGRLYFGAWDGLVYCIDAAEGN
                     LIWKKPGPAGNRSEAASKSRYYAPADCSPIIVGDRLFVCDRDYRLGSYSLTGEYHAEI
                     SEGIAAIGLTADKKGFYARGLAKGVSRYDGLGKRIWKNDEVLMGRFPVPPVEADGRVY
                     ACSNRGLLVALDADDGKLLWQYQATPQLHVMAGVALDKNGRAYVAGMDGSVTQVSVEN
                     K"
     gene            complement(18260..20353)
                     /locus_tag="P5316_08770"
     CDS             complement(18260..20353)
                     /locus_tag="P5316_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRR85089.1"
                     /translation="MKRSSVRTEVKTRLALSVCGVLGLGGVVAFGQSSFRGTWADAFH
                     IGYKNASQVDQLVGYAVQGNYNAIIAEVLAYQDTGGTGHGAYWNSQYVPKAADISESD
                     FDPLAYLCQQAHAQGIEVHAWLVTYRVSTSWPPSGNSILTSHPEWIMVERADTGGGPS
                     PIGDAYCLDPGSPDVQNYLIEIVRELVTNYPIDGINWDYIRYTQTDGGYPADSNYYYS
                     GLKRFQRIYNRSDVPSTSDSSWCTFRRRTIDELVRRCRAEIPNMPSSRQPVRLTADVL
                     AAGNYTGNFASSTAYTYFQNWKYWQEMAWLDATIPMNYKREHCTDQAQWYRNWVNASV
                     SWKNGRHVFCGQAGYLNIMPNSITQLAYALNQGSDGTASYSYYATTATEGVCESPTQD
                     PWANDWSWYPYVAANLFTSPVAIPGMPWRNPATATDGTIWGRIVDSATSLPVDDASVT
                     VGSRPVSKTDANGYYVVTLLPATGSGTSYNISVTKSGLPSGSYTGAKAVAGEVRQYDI
                     TLGSLAPQLVLNGSGTSIVLTRSVGYGGELADETFTVSAFNWPTYGPVNYQINDDSSW
                     LSVSPSYGTSSGEEDTIAIDYSTAGLPAGEYTGMITVSDPKASNTPLTITINLTVVPP
                     PVPGDFDGDYDVDQVDFARMQNCLSGATVPQNAPDCQGAKLDGDSDVDYDDMQLFAGC
                     FSGPGVPGDPDCLNP"
     gene            complement(20679..21857)
                     /locus_tag="P5316_08775"
     CDS             complement(20679..21857)
                     /locus_tag="P5316_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR85090.1"
                     /translation="MAQDFEWDEKESLGSPVMPGAAEFLHRTVAEALRKRVETGQLAG
                     GSKLPTLRKIADEFEVSTMTVRQAIRALELEGHVYRIPGVGAFVRPRAPGRVSAQRML
                     AFAASDLASAFEMGIARGVERACQKRGWTVQILDAQHEVEIEKRNMRRLPDSGSEGAM
                     VLPTWGDNSCVGALFGLQQTGYPLVIVDRIPAGLQADLVESDHAQGAYMATKHLLDHG
                     HRQVLMLTPPPLVSSIASRIQGYERALIERGITPRPEWKVWLDLQLQASAFEQHRRWL
                     GGYTAMLPVLKQTRPPVAIFAVDPYTAWGVYEACRELGLRIPQDVSVVGFDESEITQA
                     MRPPITFVSQRTADIGEAAVDLLEKRIEGGTARRDRRRIYPHVVVDVDLVERESVAQV
                     "
BASE COUNT         4132 a         6699 c         6897 g         4502 t
ORIGIN      
        1 gatgggcgac gtcgtgagtc gcgtcttgac cgaggcgtgc gaaatgctca gggcgcagga
       61 tcagggggtg ttttccctgg gtgcgggttg atcgcagaag acaccggcgg aacatgcgac
      121 ccactgcagg ccgtaagtcc ttgtgacgaa gaacaatgca acagtcggcg gacgttcttg
      181 cccgacgaag gatgctggtg tagtattacg gggctctgtg tgaaaccgaa tgccggtgat
      241 ggcgggggcg ggatgcggcg tcggatgccg tgcatgccgc ctcgctggag gtaaatacgt
      301 gggcatgagt gaagccgggc aatgtgattc ttccgccgag catcggctcg tcgggtgggc
      361 ggtgtatcat cgcccggccg tggtcctgtc gctgctgtgc gccttgatgg cgaccggctg
      421 cacgatcccg cttcgctcga cctcggaatt gcggcaaccg gcccggtgga ccaaaggtta
      481 caccattatt cttccgggta tcgagggaag gagtaatctg aacgagagta tcgcgttcgg
      541 cctcaacaag ggtggggtgc ccggggccat tgagatctac gactggacgg tcggcggcac
      601 tttcacctgg attcttaatc ttcgctggtc cgagcacaat cgcaaacagg caagccgcat
      661 cgccggcaag atcattacct atcaggacac ctatccgggt cggccggttt gcctgatcgg
      721 tcattccggc ggtggtgggg tggcagtgct gaccctcgag gccttaccgg cagagcacaa
      781 gatcgatcac gccatcctgc tggcccccgc gttggctccg gaccacgatc ttcgcagggc
      841 tctcagccgc acgaggtcgg ggatatggca ctactactcc aagtacgacg tcggttttct
      901 tcaggcggga acagccattt ttggcaccgt cgacgggcgg cactctcggg ccgccggcgc
      961 ggtcgggttc gtcaaaccct ggggaatgga caaagccggg cacgagctct acgaccggtt
     1021 actccaccag caggaattca ccccgcagat ggccgaggcc gggcattacg ggttgcacac
     1081 gacctgggcc agggaatcct ttgtgtcgcg atggctcgcc cccatcgtca agacggccta
     1141 cacgcaggtt ccggagtatg cctcggatag cggtgcttcc gccgcccaac catcggcccc
     1201 caaatgatgt gccggttcgc gtgagaaccg acgtccgaaa accaagcgag tgtcttatta
     1261 agacgtggtt tgtgctgcct ccttgcgatc acattgttgg ccagggatta cctgattgaa
     1321 acacagggac ggagaatgta gcataaacgt tccgtgagtt gtgccggcgc gaggtcttcc
     1381 tgatggggca tgctgacgtt actgtgggca aggttttgtt attccagccc gaaggctcgg
     1441 tcgtggctgg attgtccgaa ctgttcgacc ggttcggtca agtccgcgtc gaatccacta
     1501 ttcagggcgc gctcagagcg ctcgacgaag agcagttcgg cctgtttgtg agcacctccc
     1561 cggagttcct cgccgccgat cggttggcag cccatcgaca gctcggagcg gtgttggatg
     1621 tgatcgggca gggcgtctgt atcatcaacc tgtcggggga ggtgacttgg tgtaacgccc
     1681 gtgcggagaa acttcccgag gaggtgcttg aacgaattcg caagcactgc gtgacggcgt
     1741 acggctcgcc cgagggccgg gcgatcgtcg gacgttctcg aagcttgagc ctcgtggtgg
     1801 acaacgaccg ctattacgac gcctcgatca cgccgatcga ggatcgcgaa cgccaggtga
     1861 cccgcctcgt ggtggcggta tccgacgtga cccgcgcccg tcgtcttcag cagaaaatgg
     1921 atgccatcga caacgccggc cgcgagctcg tgcgcctgga tgcccaggag ctggccaaga
     1981 tggatgccga ccagcggctg gccctgctcg agcgcaagat catccgctcg acccgagagc
     2041 tcttggagtt caacaacttc gccattcgcc tgctcgacga gagaaccaac aagctggaac
     2101 ttgttctcag tgccgggttg ctgccggagg gcgaggaaat cgatatctac gcctcgacgg
     2161 aaaacaacgg catcagcgga tacgtcgcgg ccacgggccg aagctacatc tgtcccgacg
     2221 tgcagaagga tccgcgatac atcgtcggca tcagcaacgc gaagtcttcg ctgacggtgc
     2281 ccctgtggct tcatgataag gtggtcggcg tattcaatat cgaaagcgat accgccgggg
     2341 cgttctccga ggaagatcgg cagtttgccg agattttcgg tcgctacgtg gctattgcct
     2401 tgcacatcct tgacctgctg gtcgtcgagc gacatgccac gacgggccgc ctggctgaca
     2461 acgtttcttc cgagatcgcc gggccgctga gcgacattct ttccgacgcc accacgttga
     2521 tggaagatta catcggccac gacgacttgc gacaccgcct ccaatgcatt gtggacaacg
     2581 tggatcgaat acgcacgctg gtacgccagg tcgtcagacc gacgagcgga atcctggggg
     2641 ccaaaccgac ccagcccgcg aaggacccga tcctgtccgg gaaatcggtc ctgctcgtcg
     2701 atgacgaaga gattattcgc cagacggttt gcgatgtctt aagcaaatac ggttgcgagg
     2761 tcgaggtggc ccgcgacggc gtgcgggcct tggccatgat ccacgcgaga tcctacgatc
     2821 tggtgatcac ggacattcgc atgcccggca aaagcgggta cgagatcttc gcggccgtca
     2881 aggaggctca ccccggctgt ccggtgatct tcatgaccgg cttcggctat gacccgaatc
     2941 actccatcat ccgggccagc aaggagggtc tctcggccgt cctgtacaag cccttcaaag
     3001 ttgatcaact cctgactgat gtccgggcgg ccatctcccc ggggaaacgg taatcccaag
     3061 ctcgcagtca agccgaccca ccgagcggtt cgtgcgtttc cacgagcaga ccggcgccac
     3121 cgcgcctgac gattagtccg atgatgccat cagttgcccg atgagacgcc agcatgctcc
     3181 ttttaattca tcctgacact ttctgtgatc ccccaccccc gccgcggcag gggtgggtcg
     3241 ccaggcgaaa gctggcaagg ggggaaagtg ctacggaaca accatgttca ttcaagatcg
     3301 gatagcagtc tgacggagat gcctcgcgtg gctcggattg cccgatcatt cgtcagcaaa
     3361 gtatcgcact gcattgccaa agccgtggcg acatggattg catcgggcag tttcaggcct
     3421 gtcgccgcac ggatctccgc ggcctcgatc agaatcgcct gagtgatcgg ggcaagcgtc
     3481 agggccgccg aaggctcaag gaaatcgcaa tactcattca ccaaggccgc gttcttgtcc
     3541 tgttttggct tgaccagaac ctcggccagc gtcaactcac tcgtggctgc ggacagtgtg
     3601 ccttggtcca ttgctccgag gagcttgtga atgacggtct cgtgctcgac tcacccttcg
     3661 accgcgtaaa cgacgatatt ggcgtcgatg tatatccgtt tgccaagctg ggacgtcacc
     3721 tctcccatgc gttccgctcc cgacggagaa aagcatctgt ttcctgcggt gtgctgaaag
     3781 agcccctgcc ggagccgaat agcgacaagt aggacttgtc tcgggtttcc cggccgacga
     3841 ggacgatgac ttcggcctgg cgcccgacag gcaattggtc ggagatgact tcaacacgtc
     3901 cgccctcctg gattgtgaca atctgtctat atgcgctgac cattatgtca cctcatccgt
     3961 accattatac ccgaaacccg ttggcaaggg taagcctgtc cccgccccag tgtataccgg
     4021 tccgcacgac agcgacggct gagaatccgt tggtccaggc ggccggttcg gcggcctatc
     4081 ccgccagttc ctgcattttg gcgatgtaga tgggcacttc cttatgcggg tcggcgccct
     4141 cgtactcaag agtgacgtaa ccgcggtagc cggcttcccg gatgatcttg aagatgcggg
     4201 cgaaatcggc gggttcgcgc tccttggttc cgggcgtctt gacgacctcg gttttgaatt
     4261 gacagatgat cgcgtggggg gccgccttgg cgatgttctc gtaaggcttc tcgacgaagt
     4321 tgcccgtgtc gaggttgatg cccagccagt cgctcttgac cgcctcaatg atcttgatga
     4381 ccgcatcggc ggtctcggtc aggtagccgt ggttctcgat cgccaggaag atgcccttcg
     4441 agccggcata ctcgcaggct ttcttcatgc ctgcgacggc gcgctcgaag tccttgtcac
     4501 ggccggcacc gggcatggcc ttgcccgcga aaatgcggat tgccggggcc ccaagcttca
     4561 cgcacacgtc cacccagttg cgaacagact caagctcctt atccagttcc ggccctggcg
     4621 gcaggcagaa gttgttgcgc atcggggagg cgctgatttc cagtccggca aggaagatct
     4681 ttcgcttgag ggcgtaaagg tactcgtcgt cggtcttgag aaagtaataa gccgtcggct
     4741 cgatggcgtc gatgccgagc ttaacgcaga ggtcgaccag gtcaaagagc gacatttttc
     4801 ccggttggcc gggctggtcc aggaactggc gaaacgaata gcccgccagg ctgatcttga
     4861 atcgctttgg ggattgcggc cgaaccgttg aatcgccggc ggcggcgtca gcggcacggg
     4921 caaggtgggc cgtgaagacg gaggcggaca tccccgctgc agcggtcagc acttcgcgtc
     4981 gggaacacgc tttcattttg tactccttgt gtcaagatgc cacatcgacc ggagtcatct
     5041 cgacgctctt acatggccgg ccgatcgggc attagtccct tccgtcgcaa ctggtgcgca
     5101 ttttgcgcgc atgtttctgg atcgcagtgc gcttgggttg cggcccgcaa aaaagcggtc
     5161 cgcctcaggt catggcggta ttatcccccg ttggcgaagc tgtgaaaagg ttgtgcacgg
     5221 tcgtatagta gcaaccgtgg cgttgcttgc ggtgatccgg gcgatgaggg cggcgggtgc
     5281 cggccgacga gtcggccaga ggtgtgtgtc agaccatgaa ggcgaggcgg atcattccga
     5341 catattcagt cctgcttcgt accgacggcg cagaaggccc ggtcgcctgg cgcctgttcc
     5401 attccccgcg gttcatgatc tgtgccaccg cagttgagga cgtcgtgcct tgcctgcatg
     5461 agttggagcg cgcggttgcc gggggctgcc atgcggccgg tttcatagcc tatgaagccg
     5521 ccccggcgtt cgataaggcg ctggtcaccc atgcccccac gacagtcccg ctgatgtggt
     5581 tcggcttgta cgacccgccc gagaccgttg cagaacttcc aaagagcgat tcggacgaac
     5641 tgccggcgtg tcactgggaa tcgcagatga gcgaggcggc gtatcggtcg gccgtcgaga
     5701 aaatcaagca ctacatagca gaaggacaca cttaccaggt gaatctgacg catcggcttc
     5761 gctctcgcct tcagatcgag ccctacggtt ttttccgttc gctctatcgt gcgcagcctg
     5821 ttccacacgc tgcgtatctt gacctcggcc gatacgcgat ctgttcgctt tcgccggagt
     5881 tgttcttccg gcttgagggc cgacgcatca tcagccgtcc catgaagggc acggccggac
     5941 gcggacgatg gtgggaggag gatgaacgcc gcgccgcttg gttggcagac tcgccgaaaa
     6001 accgcgccga aaacgccatg atcgtggaca tgattcgcaa cgatctcgga cggatcgccg
     6061 agaaaggcag cgttgaggtt gaagaggtct tcaaggtcga gcaatatcga acactcttcc
     6121 agatgacatc aaccgtcgcc gcccgaacaa acgcgcccgt ttccgagatt ctgcgcgcgt
     6181 tgttcccttg cgcttccgtg acgggagcac cgaaggtccg aacgatgcag atcattcgtg
     6241 aactcgaggt cggcccccgt ggcgtctaca ccgggtgcat cggctacgta tcgccgtcgc
     6301 gaaaagctct ctttaatgtg gccatccgga cggccgtggt tgacgcggaa gacggccgga
     6361 tcgagtacgg cacggggggc ggtatcacct gggattcgtc cgcgaccgac gagagcatgg
     6421 agtgccgact taaggtgagc gtgcttgaac ggccagatcc ggaattctgc ctccttgaga
     6481 cccttctctg ggacggccgc aagtaccgtc ttcttgacgg acatctgcgg cggctgaaaa
     6541 ggtcggccga ctatttcggc tacccgatcg atctgcgcgc cgccggcagg acactggaag
     6601 aggcatcgaa gccctatgcc ctgagacttg cacgcgtccg actgctggtc gatgagaccg
     6661 gtaaagcgac ttgtgaatcg caccttttgg agcgatgccg cccgcgcccg acatgtcggc
     6721 tcgcaatggc ttgccgtccc gtcgacagct ccgaccggtt tctctatcac aagaccacgc
     6781 acagaaccgt ctacgagacc gcccgtgccg cctcgccggg gttcgacgat gtactgcttt
     6841 ggaatgaacg gagtgagatc accgaatcca cgatcgccaa tgttgtctta cggtttggag
     6901 accgcttggt gacgcccccg atctcggccg gattgttacc gggcgttttt cgtgaacgct
     6961 tgctcgaacg tcgggtgatt cgtgaagaat ccatgcccat cgaggcgctg gcgtccgcga
     7021 ccgccgtata cctggtcaac tcggttcggg gttgggtgcg ggcaagcctg cacgacccgc
     7081 acggaattct gcggtccgtc gggttccagc gcgatacaat gccctcgaat gaagaagaag
     7141 catcgtcaga gccgtgtggt ctggatccgc gatgaccggc cggtgacgcc ggaggaagtg
     7201 gcttggctga aaacgcctgc cgcacaggcg gtctgcgcgg ctatgatggg cgacgaaccg
     7261 gcggatacgc ctgcggcgat cgagcgctgg cggcagcggc ttgagcccga ccaggtcgcc
     7321 gccgcctggc atcagacgct cttgagacgg gcggggcggg ccaagttctc tcgggccgac
     7381 gagatgctgt tcgaccgcgt cggcctggag caggccagtg atgaggtggt ggcggcccat
     7441 aaagccgggc gattttcaga ttgcggacga attgcggatc tttgctgcgg cattggtggc
     7501 gacactctcg cactggcgac gcaagccgag gtaacggccg tggattggtc gcgatcgcgg
     7561 gttctgatgg ccgaacacaa cgcggcagtc tacggaggaa ggattgtcgg aaaggttggc
     7621 gacgtcctga tccacaggcc cgacgcggat gccatccaca tcgatcccga tcgacggccg
     7681 gaaggggcgc gacgccatga ccccgttctt gcttcacctg atctcgaaga gctccggcga
     7741 ctggtcgaac gctatcgtca cgcggccatc aagctctcgc ccggggctga tcttagccgg
     7801 ttgccgttcg acgccgagat cgagctgatc agtcatatgg gcgcgtgcaa gcaggcggtc
     7861 atctggacgg gaagattcaa gcaggtttat cggcgggcaa ccgtgttgcc ggccggagac
     7921 agcgtcttct cctcagaagt ggatccgccg gcgtggcctg agccgcggga attacggccc
     7981 ggatgctttc tctacgaacc cgacgccgcc gttattcgag ccggcctggt cggcgctgtc
     8041 gcgcggcagc tcgaactggc gccggtggac aggcagatcg cttatctgtc aggtgatcgg
     8101 ctgctatcct cagcgtttgt tacgccgttt caggtgatcg acgtgaccga cttctcggcc
     8161 cgcaaggcgc gaacatggtt ggcggctcac gacgtcggca gggtgcacat caagacccga
     8221 ggcttcgctg ccaggccgga ggaactgctt caccgtcttc gcctcagagg ccgccatgag
     8281 gcggtcctgc tcttgacgcg cgtcgcaggc cgcccgaccg ccgtccttgc ccggcgcctg
     8341 cgccgctgat tgcttgggta tttgcacgcc cgcccggtcc gtggtatgag ctaccggctt
     8401 ggggacgggt ggccggcctg gcgcttggag tattcgatga tggtcgacga gcgatgcatt
     8461 gcctacttct ccatggaaat cggcttggac gctctgatgc cgacgtatag cggcggactg
     8521 ggcgtgcttg cgggcgatac gatccgctcg gccgccgatc tgaaggtgcc gatggtggcg
     8581 gtaagcctgc tgcatcggaa gggctacttc tatcagcgtc tcgaagccag cggatggcag
     8641 ctcgaggagc ccgtcgagtg gaacgtcgac gacttcgtta ccgaggagaa cgtccgggca
     8701 tcggtgacca tcgggggccg tcccgtgcgg ctccgcgcct ggaggaggga cgtgaccggc
     8761 gtgagcggct acgttgttcc ggtctatttc ctcgattccg acctgccgga aaacgcggag
     8821 caggaccgca tgctgacgca ttggctctac ggcggcgacg acaactaccg cctctgccag
     8881 gagatcatcc tgggcattgg cggggtccgg atgttgcggt ccctggggta ccggcagatc
     8941 gatcgctttc acatgaacga aggccacgcg agcctgtgca cgcttgagtt gctggatgaa
     9001 aggcgccgtg ccggaaaccg tgacgcgatt gtacaggaag acgttgaggc cgtgcgccgc
     9061 atgtgcatct tcaccacaca tacgccggtt cctgccgggc acgacaagtt tccggtggac
     9121 ctggttgcag aagtattagg cgatcaccct gcgctgacga tgcggaacct catctgctgc
     9181 gacggcgtcc tgaacatgac ttatctggcg ttgaatctca gccactacgt gaacggcgtg
     9241 gccaagaagc acggcgagat ttcacggcac atgttcgcgt cgtacgtcat tgactcgatc
     9301 accaacggtg ttcacgcggc cacatgggcc tccgagcctt tccaggcgtt gttcgacagg
     9361 cacataccag gttggcggga ggacaatttc agtctccgct atgcgctcag cattccgcac
     9421 aaggaggtct ggaatgccca catggccgca aagcgagacc tgatcgacta cgttaatcgc
     9481 gagacgaaca cgggcatgga cgtggatttc ctgacgatcg gttttgcgcg ccgcgcggcg
     9541 gcctacaagc gagcggacct gctcctgcga gatccggatc ggcttcgcaa aatcgctcgt
     9601 gatgtcggcc cgtttcagat cgtctatgcc ggcaaatcgc acccccgcga cgaggaaggc
     9661 aagaagatga tccaacgaat ctaccaggca cgcaagtcgc tccgggatgc cgtccacatc
     9721 gcctacctgc ccaattacga catgaccctc ggcaagctgg tcacctcggg agtggaccta
     9781 tggctcaaca cgccgcaacg cccgatggag gcctcgggaa ccagcggaat gaaggccgcc
     9841 ctcaacggtg ttccgtcgct gagcgtgctg gacggctggt ggatcgaggg ctgcatcgag
     9901 ggaaccaccg gctgggccat cggccgggac ggccggactc ccgaaacttc cgccgacaac
     9961 caggccgatg cggcgtcgct gtacgagaag ctcgagcagg tggtggctcc cctgttctac
    10021 agggaccgtg atcggtacct ggacgtcatg gtgcacgcca tcgcgctgaa cggctcgttc
    10081 ttcaacacgc aacggatgat gcagcaatat gtgcttaaag cgtatttccg ttaacggaat
    10141 ctgacgcgct cacgcaccga tcacacgccg caggaacggc acaacccttc cttgcggtct
    10201 gcccgcgagc ggactgtggg ctgattccgc aatgaagggt tgtgtcgccg gggccggcct
    10261 gttcaacggc cgattatccc cacttgccct tgttcatgtg gctttggatc tcggtcatcg
    10321 agagcttcat catcacgtag gatgcttcaa ggctcttctg gaacacgtcc acgcagcccg
    10381 gatcgaggtt gtcctgaatc ccgtctttgc atgcccgctc gatctgccgg gccaggtcaa
    10441 tcacatgggc gtggagcatg ttggttcgcc gcgaacgctc gaccaggttg gcggcgtggt
    10501 cgtcgagcgc ggtgaacttc tccgccggcg caccgcactt gggacagacg gtcggaggtc
    10561 cgtcgccgtc atggatgtat ccgcatacgc acttgaactt cttcttcata ccaatcctgc
    10621 ctttcttgcg atcacgcggg tcaagcaaag cgtctcacga ttgccggacg ctcccagaag
    10681 cttaggtgac gcgggcgtat tcggcaagcc gacggtgtgg gagtctcgtc gctcctgatg
    10741 gggtggtggg gcctgctcat tctgttttgg cctcccagtg ctgcctgact ctctggaagt
    10801 actcgatccg ggtctcgatc ttaccgattt ctctcgggta gaaatcgagc atgatatcac
    10861 agagctccag catccaggat ttgcgcgaat aatgaggttt gagactgatg tggttcgcat
    10921 tagcgatctg tatctgaccc caccccagcg ccccaatcgt caggcattcc cagtcgagaa
    10981 actcgatggg tacaaaggtg acctcggaga tcaccgcgtg ggttccttcg agacggtacc
    11041 tgacaagcaa gagcacgaag tagttgctgt catcctcgta gaaacgcgtg agccgctcca
    11101 cggaggtgag attcggcatg ttgaacttcg tgtcttcccg atgggtcttg acgtcaacga
    11161 cgtagtacaa gccatcgttg tccttgaatg cgatgtcggc catggcccgg cgcgcgaagt
    11221 tggcggagta ctccgcacaa tcctctccaa ggattacccg aaagttgtcc ccaagaatgc
    11281 cttcaagcgc atcgccaaac gcgcgtgggc tgcgagccgt gctgggcgaa agaaaatcct
    11341 cgtgtccatt gaggaactcc ttggttcggc gggcaattcc ttggtaacgg tctgttgaga
    11401 aaatccggct gttcatgttt tcctttcatt tcgtggtggg catcggatcg aagaggcttg
    11461 gcgggccggc ggctggggtc cgcggtgtgc cccgaccgtt gttttggtcg gaaaagctgt
    11521 ttcgctccca gaagacgcca tcggtgtacc cttggatacc ggggtcggct tccgccaaat
    11581 gcagccgttt ttccgccaga cagcagaact cgcggtcgat ttcgatgcct acgaaatggc
    11641 gttgtagctt cttcgccaca actgaagtgg ttccagatcc gagaaaggga tcgaggacga
    11701 cgtcaccgac gttcgaactg gcaaggatga ttctggcgag gagcttctcg ggtttctgtg
    11761 tggggtgatc cgtgttttcc ggcatggacc agaagggtac tgtcaggtcg gtccagatgt
    11821 tcgagggatg agtgattcga tacctgcctt ggtcggtctg ctgccagtct tttggtctgc
    11881 ctaggtcgtc cgtgtaaggc gcaatgacgc gacgcttgag cttgacgtca tcgatgttga
    11941 agacatagtc atcggaaacg gtggcgaacc agatgtcttc cgtgttgttc ttccagttgg
    12001 tctttgcgcc gcgccctttt tctcgctccc acgaaatgcg atttctgact ttgtagtggc
    12061 gaagaagcac gcgatgtatc gccgtggagg atcgccagtc cccgcagacg tagatggacc
    12121 cagtaggttt caaaaggcat cgcagccccg ggagccacga actcaaccaa gcctcgtatt
    12181 tgtcaagatc ggtctgtttg aaggtattac cgttgaatga tttcgtcagg ttgtaggggg
    12241 gatccgccac gatgagatca aaggcagcac gaggaagaag gggcagcact tcgaaagcgt
    12301 cttggcagat cgttctgtct aagacgtctg gcagcgacgc tggggagtcg aggcgaagca
    12361 atgactgcga gcagcgccgc cggtcggcgt cggacaaaac aagggttctg tttcgtggtg
    12421 ctcgcggttt ctcgttcatg aaggtcttgt atcctctttc cgggccgctt tcaagatcct
    12481 ctgcggattg gtatgccagc ccggatgtac ccatgcgagg cctccgagaa tcttgggggt
    12541 tccgcgccac ggccatgcgt ttgttcacgg cgccgccttc gtctccctcc atacttgttc
    12601 aatccgctgc ttgagcgctt cttgcgattt ctcgtaggac gccagcgttt tttccaggcc
    12661 ctccagcagt cgcgttgccg tgtccaggta ggatttctcg gcggccgctt gcgacttgtc
    12721 gggcaaggga gcgagcttgc cggcttcctt caacttgcgt cgttccagga tgaccgccgc
    12781 gcactcggcg gcgagtcggg catgggccgc ctcgtaatcc gccggcacct gctcccgcag
    12841 cacacgatca tgaaatgact gcagcctggc cagccgcggg gcgagcttgc cgagttcagg
    12901 atcatcgacc gagatcgcag gccagggact gccgatttcc gccgggccgg ggcccggcgg
    12961 ccggctgacg tttccaagct cgatgtgaat gaacgaggtt tcctgaaccg ccccgtacgg
    13021 caacatcact gattcggcgt cgaagttgtc gtaggcccgg ccgtcgagcc gcacgccggt
    13081 taccggccct ctgcccgaaa tcgaaagaaa cagccgcgac ttgccgaagc gaatcgggaa
    13141 cttctgctcc aggaacgtaa tgcccggggg aatatgcggc accagccgca acccctccgc
    13201 ggtgtacaag tattcgaaca gcccgcgaac catggcggcc gccgggccga acgtgtcgta
    13261 gcagatgttg atcggctcgc ccggctggta gacgttgtcg ccgaaatcga ccagggggtt
    13321 atccattcgg aaagtccgcg cgaacttcat gagctgcctc atcgacctga gaatgtcgtc
    13381 gtggcggccc aaccggtagt aggccatgat catgcgggct tcgcacgtcg accagtggcc
    13441 gccgttgacc cagcggccga attcccagag ccccttcggc tgctcataca tgtcgtcgag
    13501 ggaggggaaa ttgggtatca caaaagcatg cgggcgaagg ccggggatgg aggcaatctt
    13561 cgcgtagatc cgctcggcct gcggcttgtc cactacccgg aacgcaatcg cgtcgtggtt
    13621 gggcgaggtc tcgaagtatc cgtgcctgct cgctccgaag acgccgtggc gcgtgccgtc
    13681 ggggtcgagg ctcttgatga agtatccctc gtcggtggtc aaccgcggca ggccttcgcg
    13741 ggccagctcg cgccgctcgg catacagctt cgccttgtcg gaaaacgccg ccagtttctc
    13801 aagttcgatc aggcgatcca gggctgcgat ataggtgatc gacagacccg cgaggtacgc
    13861 cttgtcgtat gtgccgtcgg gtttcttcca gcccgcgtag ctcggtgcca gcaggtttcc
    13921 ggccgggccc gccaggaaca gattgttttt cgggtcgcgt cgcgactcga tgaagttcgc
    13981 actccgttcc agcttcggca gatacttggc gattgcctcc gggtcgcggc tgatcagcag
    14041 cagctcggcc tgcatcagca cgccggcggc ggtgaattcc atgccccagt catggatgtc
    14101 cacacggccg tcgccctgct tggcgacaaa ccatcccggg ctggcggcat cgcacaggca
    14161 gccatccggc ggaacccagt cgaacttcgg cccgctgccc atctggaacg gccggacggt
    14221 cttgccgtcg cccatctggt cgaaccacag gtcctgcgag ttctgaagga acgtggtcat
    14281 cggctcgatg tagaacggca gagcgcagta cgtcgtgcca tagctgttct gaatccacca
    14341 cgctccccaa gtcggccctt cgaccagacc gttccacgcc ggcgtgtaca gcatcgccat
    14401 gttctggaac tcggggtcgg ggtcaaacat ccgccgggca acgtccagca tttcgagata
    14461 ttccacgtcg ccgctgccgg caaagtactt gccggaaaag gaatgggacg ccagactcgg
    14521 catgaccgtg gcaagactga cgctcagcaa agctgtcgtg acggtgattc tctgatgagt
    14581 gaacatgtca cgtctccgta agtgctactg gcggcacgac tataccgacg ggcgggtcgt
    14641 aaatcgagcg agaatgtgcc ggcgtgttca agtatcatcg aagttgcatg gacggtgttt
    14701 tcatccgagg gtcgcggaag aatatcggac cgtcgggatc tgccggcggg cgccgacgac
    14761 aatccgatcg caggctggac tgatctcggc acgttcgacg gaccatggaa tagcggcgac
    14821 cggagtatcc atgcccgtac gggcccggag tgggtcggct cgatcaccaa ccgtacggat
    14881 gggaagaagg acgtcagcta ttgcgaagga tcgcgatcgc gccttgaagg ctgagagaac
    14941 aggtctctcc tggccatcag tccgaaggct ccgcccgtgt ggatgaagac cggggtttcg
    15001 tcgggtcgca ggccgccgtt gcggatggtg gccagcatgc cggtcatggc cttgccggtg
    15061 tacaccggat cgaggagcat tccttcgctt cgagcgaccg agcggatggt ttcgacctcc
    15121 tcggggtagg ggatcgcgta tccctcgccg atgaagccgt cgatcaggtc gatcggtgtg
    15181 tcggcctcgc gcagcccgag gtcgaaccgc tcgttggtct gtcgctcgag cttgcggatg
    15241 tcttcgctga gaatctcgac gttgtcgctg accggtatgc ccctgatccg ccagttgcga
    15301 caacccagca gggcacggcc gatgctcagg ccggtatagg tgccgaccga gctgacggcg
    15361 cagaagatcg ccagcggcgt atctcggccg atctgctcga caagctcggc aacacaccga
    15421 atgtaccccc agcagccgag cgggattgac gctccgaccg gaaagaagta cggccggcgc
    15481 ccggcccggc gctcggcctc catggccgcc tcgatcatct cgctgcggcg cccattgtac
    15541 tcatcgggat ggtgaatgct cacgtcggcg ccgaacaggc gatcaaggaa cagattcccg
    15601 tcgtcggcgg ggtgaggatc tgcggaccgc agcagcagcc gaacgcgcag gcccaggctc
    15661 gcaccggccg ccgcggtcgc acggcaatgg ttggattgaa aaccgccgtg cgtgaccagg
    15721 gtgtttgcac cggaggcaat cgcatccgcg agaatgtatt cgagtttgcg gatcttgttg
    15781 ccgctcagct cgaagccggt ctggtcgtct cgcttgacga gcaaacgcga aattccgatc
    15841 tgccgcgcgg tgttcttgag ttccgtcacc ggcgtcggaa tccgggccag gctcagccgt
    15901 tccggctcct tggggtgcag tctctcgtgc gtcgccattc acttgttctc cacactgact
    15961 tgcgtcacgc tgccgtccat tccggccacg taggcacggc cattcttgtc caaagcgacg
    16021 ccggccatga cgtgcagttg cggcgtcgcc tggtattgcc agagcagctt gccgtcgtcg
    16081 gcatccagtg caaccagcag cccgcggttt gagcaggcgt aaacgcggcc gtctgcttcc
    16141 accggcggga ccgggaatcg gcccatgagc acttcgtcgt ttttccatat ccgcttgccc
    16201 aaaccatcat agcggctgac gcctttggcc aaaccgcgag cataaaagcc tttcttgtcg
    16261 gccgtcaggc cgatggccgc gattccttcg ctgatttcag catggtattc gccggtgagc
    16321 gaatacgatc ccagtcgata atcccggtca cacacgaaca ggcggtcgcc gacgatgatc
    16381 ggcgagcagt cggccggagc gtagtaacgc gatttcgagg ccgcctctga acgattgccg
    16441 gccggacccg gcttcttcca gatgagattg ccctcggcgg cgtcgatgca gtacaccagc
    16501 ccgtcccagg cgccaaaata cagccggccg tcacggagca gcagcgattg ctcgatgctg
    16561 aaggtcgcgt ggcgtttcga ccacaggctc tgccccgact tgcgatcgat cgcatggaca
    16621 aaaccctcga ggtcgccgac gtacacgcga gcctgatcaa cagccggcgt gccatagacg
    16681 acgttgcccg gcgcgggcgg cttccattgc cacaccggtt tgcccttcgc gctgatcgcg
    16741 tatacgcctt tctcggccgc gccgatgtag agaacgtcgc cggcctgcgc aggagggtga
    16801 agaatttccg acccgacgtc gagcagccga gtgatcgtgg gcacgggctc cttgaaagat
    16861 accctgacga cctcgcccgt ggtggtacaa acgaccgctt cctgatcagc ggcgagcggc
    16921 gtcgccttaa cgccggccgc caattgcgcc acccgaccga ccggcccggt gcttttgccc
    16981 ttgtaggtga attcacacgc ccgatcaagt tgataagggc ccatgtcggc catcacgcga
    17041 acaaagtgta ccccgggcgg caggaagccg gccggcaggc ttccggagaa cgtgcctgcc
    17101 tgattgtcgc ccttgagcgc gacaccacct tggtcgtcgc cgttgatgca ggcggtgacc
    17161 gattggggtt gtcctccgct gatccgaaca cgcaccggca ccccggctgt gcgaatcatc
    17221 ggccggttgt cacctttacg cggaggcaat tgaaactcga gttgcgcctt gtcagccggg
    17281 gcgatgggct tctcgatcag aagtgccatc ggcttgctcg aatcgtggaa ccggtatgtg
    17341 gaacgaagca cgccgttcat gacggagatg attccgtatc cggtgttcgg accgtaggtc
    17401 gagccgccca tgacactgtc gatcgtgccc catctctcat gtcgtgcccc gtggccgtgg
    17461 cccatgagca gcagaaccac gttccggctt cccagcagct cgatgagtcg aagctgctcg
    17521 taaggcttgg cgaactcggt gctggacagc gggtggtggc acaggacaaa caccggagtg
    17581 gtcttcgaga ctttcttgag gtcgtcggcc agccaggtga gcgtacgctg ttcgatggtc
    17641 ggcaccggct cttgcggggt ggccgtgttg atgaacgcga agtggcagcc gaaccgatca
    17701 aaagaatagt ggtcgccgca gtgcctttga cgcatgattc tctggatggc cgtccaggta
    17761 ttgtcgtggt tgccgggggt gatgtaccag gggatgctca ggggcttgaa aagatcctcg
    17821 acaacgcccc aggtctcgtt gatgaccccg tactcggtga tgtctccggt ggccaccagg
    17881 aacgacggcg ggggcgtccg catgttgagc ggcccgagca cctgcggctt ggccgcctcg
    17941 tcgcagaccc aggcgattcc tgccgcgctc agacgagcgg cgtcctcttt gccggtgccc
    18001 aggggtacgg gcgagacgtg catgtcagag atgtgcagga aacagaagtc ctgcacggta
    18061 ccgtctgccg ccacggccga agccaggact gcggccggcg tcaaagcagc cgctaccagc
    18121 atccatttga agactcgcga acaatgtcga acgtgcatca tcattcctcg acgcaaacgg
    18181 gaaacgccgg atcatgctag gggacgcgca gtctgctttc cagcggggcg gccgactccc
    18241 accaccccgg gcgcgaacgc tatggattga ggcagtccgg gtcgcccggt acgccgggcc
    18301 cgctgaagca gccggcgaag agttgcatgt cgtcgtaatc gacatcgctg tcgccgtcga
    18361 gcttggcgcc ttggcaatca ggggcgttct ggggaacggt cgcgccgctg agacaattct
    18421 gcattcgggc gaaatcgact tgatccacat cgtagtcgcc gtcgaaatcg cccggtaccg
    18481 gcggcggcac aacggtcaga ttgattgtta tcgtcagcgg ggtgttgctg gccttgggat
    18541 ctgaaacggt aatcataccg gtatattccc cggccggcag gcctgcggtg ctgtaatcaa
    18601 tcgcgatcgt gtcttcctca ccgctgctgg tgccgtaaga aggcgaaacg ctcagccagg
    18661 aggaatcgtc gttgatctga tagttgacag gaccgtaagt tggccaattg aatgccgaca
    18721 cggtgaaggt ctcgtcggcc aactcgcctc cgtagccaac cgaccttgtg agcacgatcg
    18781 acgtacccga tccattgagc accaactgcg gggccagcga acccagtgtg atgtcgtatt
    18841 gtctgacttc cccggcgaca gctttggctc ccgtgtagct ccccgacgga agcccggact
    18901 tggtgaccga gatgttgtaa gacgttccgg aaccggtggc cggcaacagc gtcaccacgt
    18961 agtaaccgtt ggcatccgtc ttgctgacag gacgcgagcc caccgtcacg gatgcatcgt
    19021 ccaccggcag agaagtcgca ctgtccacga ttcgacccca gatggtgccg tcggtcgccg
    19081 tggccgggtt gcgccaaggc atcccaggga tcgcaaccgg cgaggtgaac agattggcgg
    19141 ccacgtaggg gtaccaggac cagtcgttgg cccagggatc ctgggtaggc gattcgcaga
    19201 cgccttcggt cgccgtggtc gcgtagtagg agtagctggc ggttccgtca cttccctggt
    19261 tgagggcata ggccagttgt gtgatgctgt tgggcatgat gttcaggtaa ccggcctgac
    19321 cacagaagac atggcggccg ttcttccaac tgaccgaggc gttgacccag ttccgatacc
    19381 actgcgcctg atccgtacag tgttctcgct tgtagttcat cggtatggtg gcgtccagcc
    19441 aggccatttc ctgccagtac ttccagtttt ggaaataagt gtaggcggta ctcgatgcga
    19501 agttgcctgt gtagttgccg gcggccagta catcggcggt caatcgcacc ggttgtctcg
    19561 aactgggcat gttcgggatc tcggcacgac agcgccgcac gagttcatcg atggttctgc
    19621 gacggaacgt gcaccatgac gagtcgctgg tcgacggcac gtcgctgcgg ttgtatatcc
    19681 gctggaagcg tttgaggccc gagtaatagt agttgctgtc ggccggatag ccgccgtcgg
    19741 tctgagtgta ccgaatgtaa tcccagttga tgccgtcgat cgggtaattc gtcaccagct
    19801 cgcgcacgat ttcgatcagg tagttctgca cgtcgggcga gcccggatcc aggcagtagg
    19861 catcgccgat tggactgggg ccgcccccgg tgtcggctcg ctccaccatg atccattcgg
    19921 ggtgcgaggt gagtatgctg ttgccgctcg gcggccaact cgtgctgacc cgatatgtga
    19981 ccagccaggc gtgcacctcg atgccctgag catgggcctg ctggcacagg taggccagcg
    20041 gatcgaagtc gctctcgctg atgtcagcgg ccttgggcac gtactgtgaa ttccagtacg
    20101 ctccatgccc ggtgccgccc gtgtcctggt aagccagcac ttcggcgatg atggcattgt
    20161 agttgccctg aaccgcgtag ccgaccagtt ggtcgacctg cgacgcgttc ttgtacccaa
    20221 tgtgaaaggc atccgcccag gtgcctctga aggaactctg gccgaaggcg accacgcctc
    20281 caaggcccag aacaccgcac acggacaacg caagccgggt tttgacctcg gtcctgacac
    20341 tgcttctctt catgattcac tcgccgcctc cggacagccc gccggttcac ggatttccac
    20401 ccatatgacg gtctgcaacc ggaacggcct cctccgcact aaagaattct acgcgcacct
    20461 actgtccgat tcaacgctta ccgctctcgt tctcggaccg aatccgcccc attcagtcgg
    20521 cgcaggtgcg atccgcctgc acgtctggtc cctgcatgca ttccagcaag acagcgacat
    20581 cgtcctgagc cacgtctgaa tcgccgtcca actcggcccg cgcacgcgcc tcgtcgttct
    20641 gcgaaacctg cgacccgctg aaacgggcct gctctcgctc acacctgggc gaccgattct
    20701 cgctcgacga gatcgacgtc gacgacaacg tgggggtaga tccggcgccg gtctcgccgt
    20761 gccgtgccgc cttcgattcg cttctcgagc aggtcaaccg ccgcttctcc gatatcagcc
    20821 gttcgttgtg aaacgaacgt gatcggcggc cgcatcgcct gggtgatctc cgattcatcg
    20881 aatcccacca cggaaacgtc ctgaggaatc ctcaaaccca gctcgcgaca ggcctcatat
    20941 acgccccagg ccgtgtacgg atccaccgcg aagatcgcca ctggcggccg cgtctgcttc
    21001 agaaccggca gcatcgcggt gtaccccccc agccagcggc ggtgttgctc gaaggcgctc
    21061 gcctgcaact gcaagtcgag ccagaccttc cattcgggcc tcggggtgat tcccctttcg
    21121 atcagggccc gctcgtatcc ttggatgcgg gacgcgatcg aggaaaccag cggtgggggc
    21181 gtcagcatca gcacttgtcg atgcccgtga tccagcaggt gctttgttgc catgtacgcg
    21241 ccttgtgcgt ggtctgactc gaccagatca gcctgcagcc cggccggaat gcggtcgacg
    21301 ataaccagcg gataccccgt ctgctgtagc ccgaatagcg ccccgacgca cgagttgtct
    21361 ccccacgtcg gcaagaccat cgcgccttcc gagcccgaat caggcagccg cctcatgttg
    21421 cgtttctcga tctccacctc atgctgagcg tcgaggatct gcaccgtcca tccccgcttc
    21481 tggcaggccc gctcgacacc ccgggcaatg cccatctcga aggcgctggc cagatcactg
    21541 gcggcgaagg cgagcatccg ctgggcgctg acgcgacccg gggcgcgggg ccggacaaac
    21601 gccccaacgc cgggtatgcg gtaaacgtgg ccttccaact caagcgcccg gatcgcctga
    21661 cgcacggtca tcgtgctgac ctcgaattca tccgcgatct tgcgcagcgt gggcaatttc
    21721 gagccgccgg ccaactgacc ggtctcgacc cgctttcgca gggcctctgc aaccgtcctg
    21781 tgcagaaact cggccgcccc gggcataacc gggctgccaa ggctctcctt ctcgtcccat
    21841 tcgaaatcct gagccatacc gccatgatat cacggcgcga tagaaatgca ataggccata
    21901 atccagaaaa ctctgctgta tcacgatagc tcacaggaac gccatgctct aatcgcggga
    21961 gttggcgggc ttcggaccgc tcgttgacgt cggcacgggc ccgagaataa ccataagttg
    22021 ttattggaaa atggtttatg tgaattgtta agcattttta gcgcgcagag cggttttttc
    22081 tatcacaaca tgatgattcc cccttgacat gtgacacgcg ctgcggtagg ctgtactcag
    22141 cgctgggtga ggtactagat ttgccctttg cggtgtattt tcgaatttat gtccgacaac
    22201 ccggcaagtt gtgcgataga atttctgagg
//
LOCUS       DAOTPW010000076        22094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2424_length_22094_cov_5.491129, whole genome shotgun sequence.
ACCESSION   DAOTPW010000076 DAOTPW010000000
VERSION     DAOTPW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22094
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_22094_cov_5.491129"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1493
                     /locus_tag="P5316_08780"
     CDS             249..1493
                     /locus_tag="P5316_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85091.1"
                     /translation="MIAERPLRWPMAILLILPATAIGAPVMIEAIPGQAELGTVEIHG
                     VSAGPFDPAIWRSGSLNLVPDIRHPLLAPCQGGGFRNIYAPSPVEVPGGWRLFYGAWD
                     GVQSGNDRIYSRFTADFIDFGERRIEIEHGTFIHVCNVNAVRLPDGSFRMACTAYPDT
                     NGQNKPAIFTSPDGRAWNDKPAPYPATASDIIQIEGYPNYDAADINGMNVLRHENGKY
                     RLYFGDFKARLQVHRASSDDGRRFTYDGVSLDAAMMVNDVRDFIVDGQPVYLMALHAN
                     TSRLWYALSGDGMEFEPQREMAVSRSDADKYIVAVGWVSNGDRLLGFVYGAGAVPSLD
                     RNRLFARWLQKKLVFTDRQGNTLTVAGALGPDRQIISIPARETEGRLEILSEDGKTRL
                     GEPLDLKLTAGGMYRLTWRVAR"
     gene            complement(1633..2238)
                     /locus_tag="P5316_08785"
     CDS             complement(1633..2238)
                     /locus_tag="P5316_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85092.1"
                     /translation="MAIDRKRVIQSLSKIQVVGNDQGLIPAFGVLVNQLPTKFWNGFG
                     ERLTRGVGPDTLEAAEWLLINAAHECGYHTGHGIITSEEWKSVVAPMVENTEDVLHGA
                     FAVFTAWGWAKSEIVELVPNEKMVVRAYDYYEQDVVSYGASAKPSAYMIRGVCAAFMD
                     LAYGGKYDPTGKTGMRTFTCQQTKGIECGDEYGEFVVTRAA"
     gene            complement(2257..2793)
                     /locus_tag="P5316_08790"
     CDS             complement(2257..2793)
                     /locus_tag="P5316_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HRR85093.1"
                     /translation="MPVMEETQLILDHKFDPQTCRHMVNGELMVMHCHHFITLYTQLA
                     EDCGMLDGKKLMAEVAEDTFNEMLNKYYREHEVTDVTDRITIAEQYYSAIGLGQMKVL
                     CAGADGGEVELVHSHVDEGWLKKWGKHETSVNYVTSGYIAGLFSAVFGQPTRHFNAVE
                     TQSIVCGAKSSRFQVVAN"
     gene            complement(2809..3000)
                     /locus_tag="P5316_08795"
     CDS             complement(2809..3000)
                     /locus_tag="P5316_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85094.1"
                     /translation="MAQKLTAFYEFVGANGGLEAKMRLAMMTAIPSNKAGEAPDSPEN
                     IAKFKDAVKKITGKDAPIA"
     gene            complement(3037..3351)
                     /locus_tag="P5316_08800"
     CDS             complement(3037..3351)
                     /locus_tag="P5316_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85095.1"
                     /translation="MRIIAVRKIEDCFDGSGVYGYVFDVPWTPETIQLLRTLGPVDYF
                     SEFPRPFFRLRTECGCQIKGVEGDCECRAIYPRDEREVARKEFEQLFDRGSEAFRGSG
                     DT"
     gene            complement(3348..4517)
                     /locus_tag="P5316_08805"
     CDS             complement(3348..4517)
                     /locus_tag="P5316_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRR85096.1"
                     /translation="MGVIGVAVARRGVWRAYLNNAASGYPKAPGVIEAVVRVLSQSPE
                     HPGRATASAEDVLTDCRMRCAAYLGVRKASRIVLTAGATQALNMGISGLRLKRGSLVV
                     TTVTEHNSVLRPLNHLVRTTGIRLSIVGLAEDGALDAETISKELEREPALVVFNHASN
                     VTGRINDVSHWFSKAKAVGAVTMLDASQTAGLIPVNPDDLHADLTAFPAHKGFHGPPG
                     IGVLYVAEGIDLEPLIVGGTGVRSDLPYQPEDMPARLEAGTPNVPAAAGFAAALDWAT
                     GLDSGARERAERVAVTLRKGLLEIPNVKVFDGKSGAQQIGVVSFVVKGWTVEEMGYVL
                     QECYGIACRTGLHCAPLIHRAIGSDPEGTVRFSTSAFTSEEEVAVALDAVRGLAA"
     gene            5244..6479
                     /locus_tag="P5316_08810"
     CDS             5244..6479
                     /locus_tag="P5316_08810"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRR85097.1"
                     /translation="MARTLLASLVVLFVVPMVIAQPTTAPAGYGGSWTEWSKATGDWG
                     GARTALADKGITLDWGITQIVQENMHGGADTNSAIKYSGSSSLELKLDTGKMGLWPGG
                     TFVFHGEPRWGRGINRFVGSLMPANMDALMPGGDDSCEMTLSEFFYQQVLFEGKLVLL
                     AGKLDGSRAFDTNMFANNEETQFMNVALRNYPMIPQFIPYTTLGIGIVFNPFEWWSIR
                     TAVVDSDGSATRTGFDTAFHGDANTSVMHEWDFTVKPFGQLGHQRVAMITSCKDTPTY
                     TGGMQNCNLAVLYNFDQYLWTESEGSDQGVGIFGRFGWADKDVNPVEYFYSLGVSAKG
                     LIPTRDRDTCGLGWYFGNASNQLAPAGIVQESGIEMYYSIEVFPWLHISPDFQIICNP
                     GGGNADTALVGGVRFQMDL"
     gene            6936..8291
                     /locus_tag="P5316_08815"
     CDS             6936..8291
                     /locus_tag="P5316_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRR85098.1"
                     /translation="MNSNDIRLVLSVLVLLSAIGGLQMAVAADPEAIRMDPAQLVSDT
                     LEPAVARPLGGGEQGIPETQPASADWPPGLMMDGLDAISLGRPMRDLGLRLYGHVEAG
                     MTFELTGPGDKNTGLPLRGFDSRRPQNLRLNQLMLSLDRPVDTSKSFDVGGTFTFLYG
                     TDARLTHSLGLLDKQNHEAQPDILQGYGELWFKTGDAGQGLDVIFGKWFTPAGFEVTP
                     APANYLYSRGYLFTFAQPVSHTGLKLTYFFDPDNYAYFGVVRGWDVFDDNNDGATFMG
                     GFQLSSKQLMGDNPRSQLAVNVIAGPEQTGEGWPGNRVLVDVVWTWRWTEKLTQHINA
                     DYGFQDDVPEALGNRDVPGVRDSAWYGLGYWLNYTFNDYLSATGRAEWFTDNHGVRTG
                     YRGTFFELTTGLSITPFPNDRILKNLSIRPEIRADWSANDAPFAESCQMTAGFDVVYK
                     F"
     gene            8509..9354
                     /gene="murI"
                     /locus_tag="P5316_08820"
     CDS             8509..9354
                     /gene="murI"
                     /locus_tag="P5316_08820"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRR85099.1"
                     /translation="MNDRPIGVFDSGLGGLTVVRALQEQLPNETVIYFGDTARVPYGT
                     KSSETVLTFAEQDCSFLLRFDPKLVIVACNTASALALDYLKEKMPVPVLGVVEPGAAE
                     AVRRCQGRGIAVLATEATVASNAYAKAIAALAPNIPVAQQRCPSLVALIEEGRTWDDP
                     IMTMLVAEYLEGVKDLDPAVVVLGCTHYPLVAKAVAKQMPHATVVDSAGATALAARDL
                     LVREEAMSDASAPGRLRCYVSDNPQRFREVGGRFLNKPIRDVTSVTPEQFFLEQVLET
                     AGSMT"
     gene            complement(9554..10333)
                     /locus_tag="P5316_08825"
     CDS             complement(9554..10333)
                     /locus_tag="P5316_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRR85100.1"
                     /translation="MIREKHHRRCLAIVLLSLGASYGLVITVRAALPETRPAAAQDKW
                     VKHYHDRVELFKKENAAAKNIVMVGSSHIEGFDAARLLPGRRVVNRGIASDRIGLTDR
                     GILHRLDSSVFDCNPGFIILENGVNDLGELQRNGTPTIEEIDACYRKVVKTIRDRLPD
                     VPLVIVGLFPTRDRFDQLTPGIREFNKRLAKIAADHNCSFMDVFKPLADEQNLLRKEY
                     SRDGLHLNEAGYKVWAKMIEEILPPLNPAPRFSNESEQERS"
     gene            complement(10330..11679)
                     /locus_tag="P5316_08830"
     CDS             complement(10330..11679)
                     /locus_tag="P5316_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR85101.1"
                     /translation="MKPLACVSNRASNCETGTVRTVLLRLIPFIVILYVFNYLDRVNV
                     SFAKLEMKDDLGFSESVYGLGAGIFFISYFLFEIPSNLIMQRVGARLWLARIMITWGL
                     ISSAMMFVRGPYSFYTLRFLLGAAEAGFAPGILLYLTYWLPVRHQAKAVAWFLTSTAI
                     AGAVGSPLAGLLLKLDGTAVLGRALAGWQWLFLLEGIPSVLGGFAILLLLPNRPEQVK
                     WLSPEQQRWLNSQVRADRERLKSIGHTSFFRAFRSPTFLMLNIIYFTLMFGFQGINFW
                     LPTIVKQVTASRDNLHVGLIASVPYFTAMIAMVLIGRHSDRFGERRRHVALCAGGGAL
                     GFMLCAMTDQPVAGIVAMALAASAIWSTLGPFWAMPPAFLAGTAAAAGIAMINSIGNL
                     GGFVGPYLIGVTVDQTRNYKAGLFVAATSLLVGAVLTLLLNRVREQHHVSLKEEPDS"
     gene            complement(11786..14455)
                     /locus_tag="P5316_08835"
     CDS             complement(11786..14455)
                     /locus_tag="P5316_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85102.1"
                     /translation="MTTRGLIVIGLVCLVAPGTLVGQKAEGEGKPRGRQLFYRMVGSR
                     NNAPILPKTRSVWTQDKRVEIGNTGMADEGYTEFPFCYGLMDFTFVEHGPIYPRIISG
                     AQADESRINSADLVIDHVYQPDGCVWGWSAREFVQTFTATSTELVSMTLMVASEPGIF
                     RAALVEGGPGGRQIGPTKTFVSGHSMEWGIARWITGQAPMVPGRTYGIRLWREDGKAW
                     MPYLHSIGNAYDGGVLYVDGLPRPESDLAVWIVEEPADLKRSPVENTDEEGWVYDTDR
                     IWFTPRTPNVRMVSLIVSPILMNPPTEHNCCDLVIRVRSADGKIVAGPKRGLSCGPEN
                     GPHAAPFLFAADEFPVAVGERYQIEVYAVAHKAPLPKDDHVVIIRGDMHTTVYGEPQP
                     GALPAIFNLTAATPQDSKLKLTWSQNFPTQTRIEIRGPGMKGAGPDLQRVDVEKGRTE
                     ATIPVWSGHDYEFRLTATGPTGLVWRTPLYRIRMPRANEIEAVLSSPEPYPPCFVNLA
                     PSRVAAGTDPEPLRYRRAVELVNPEFEEGLIGWQVSPPNALDSPDVGVQSRSELKQLG
                     IGTRWGSRMAGFTHMAGEKREQVFAKSTLAQKIATTPGHAYALAAMAFTAVKNGPRGD
                     TRVRLFVDPDGGDNFDGPNSSQWYWTDGKWIRFEHRWIATAEQATIGFGFFRWRDLDA
                     ASAYVDHVHVYDLGPAPAAADAPPVRTAERLSLVLIDHKIEADDRVEGFIQAPPGYVI
                     TGLGARAHYDNITTMWMKIQPLMPDGSLGEPEELRTGWERDAGLEAQIELPAGYVATG
                     FGAGIAPEWDVKRMGVWARPLNKDGTLGEEKLFRGGVDLQSGFEKQVQLPAGRVLTAA
                     GLNCMLNDVNGIKAASAALIRTAEARAKSSAKQ"
     gene            complement(14587..15570)
                     /locus_tag="P5316_08840"
     CDS             complement(14587..15570)
                     /locus_tag="P5316_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206773.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRR85103.1"
                     /translation="MTDSPRYYPFSRYLRRRYGCRVHKVTIHAGFTCPNRDGTRGYGG
                     CTFCNNAGFSPNARTDPAVVREQLQWGIEQTRRRSRATKFIAYFQAYTNTYAPLEHLR
                     ELYDEAWKFPEIVGLSIGTRPDCVDPAKIDLIAGYSGRGEVWLEYGLQSAHDATLKAI
                     NRGHGYQEFLDAVEMTRDRGIKICVHTVLGLPGETREMILDTHRRLAQLPIDGIKIHL
                     LHVMRDTVMAAQYARGELALLGRQEYVDLVCDVLETLPATMVIQRMHADAPRDILVAP
                     EWCLDKAAVLEDIKRALARRDTWQGKALGFALSDIPTLPPAPAMYHHRSGV"
     gene            complement(15974..17248)
                     /locus_tag="P5316_08845"
     CDS             complement(15974..17248)
                     /locus_tag="P5316_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85104.1"
                     /translation="MIRVACPHCQRRYRTRTEGMGKTAVCTGCHQTFRIGQVRLPFEW
                     KQTDLGEDSWIGVEAPKEKKELRNCIICQAPLDDEAITCPECGANQVTGIVRRPPRRP
                     VEVKPSVLSALPWKLIAGIAALAMLSIGGYRLFKTATSSALDSGEKAAHMATTRLAAE
                     HLRKGGDESSLAAEFAGRVDDGNLPVFLDMLSAGRQDIRQAAVLLIGCGNVTNLRPIL
                     DMAGSAAGREQGTAVLEAVNPRRLAVLSNHEEANVRMAAAEGLCILSGLKPESEVLNQ
                     LAEKAPWEEKISRLNRFCRPYPAATGRFSVTINDTAAPFVVSIEQIGTWFFMKLDRAE
                     FHTEPRQTRTFEIPIDRWCVATGMALDAAAIRRMLTGSVRLVSPIGASWRGTVTVSAR
                     IRLDSPLPGFLPVDPPAAGQTAEAEITLTRIP"
     gene            17553..19235
                     /locus_tag="P5316_08850"
     CDS             17553..19235
                     /locus_tag="P5316_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85105.1"
                     /translation="MTEGIQQKKRWPWWLRLILYLSPLWIILAIILSVEISGRRAYNR
                     AIQEARDIGGPVTIEEIEAARKVWPDDENGALVLLRVRDRLDAIGKEAAATTLPVLGT
                     LKDELGYRWSDDTIQAVEAFLQQHAAVFAEIDRLRQYDGGRIPFEMKPNPLDTCLPDR
                     AFLRHSAKLVFLRLESRIILGDTSRAVDDIDLLFRHARLLDGEGTLISSLVRLAIGAL
                     AENGVQRVLSLCSLSDQQLQQLDEMLAAFERTDCLEIGYRGERAFFLMLTEEANLRRA
                     VATGNNPKFATLPMELPVLHGVFQMDRAAGLRMLNRIVKSNTLQDRLKAMMENEAEKA
                     SVPRLYLATRIMIPSLERSVVLDQNRIAQVRAARTGLAIERFRSDHGRFPQRLEELVP
                     QYLPALLIDPFDDKPLRYRVTDDAVIIYSIGEDGIDDGGYVGHRTAPETSPTDWGFVL
                     LKPDFRGRPASTTAPATQEAETAAVSTGPGGVGHARAESRECHPAMQTGESGHTSALH
                     QRHYTGSRETGRREQRQPCDAARFHITSHRSPIAAGGAARWWRRKGLSHDRD"
     gene            19222..20667
                     /locus_tag="P5316_08855"
     CDS             19222..20667
                     /locus_tag="P5316_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85106.1"
                     /translation="MTETDKNKKRWPWWGRLLLLLSPIFVLLLVIVVFEAGGRRSFNK
                     ALQDARDIGGPVTFEEIEAARKVVPDDVNGALIITSLTGPLSPSTDNPAHELLPLLGQ
                     ADLPPLGEKWPDQTDQVVRDYLARLSAELSKIDGLARCESGGMPFQVAPNPFDTLLPS
                     LGHVRESCKLKSLQVISEAMQGNTKSLVTDLDILLRHGQTLADHPTLISSLVHVATDS
                     LALDVLQRVLGQTTVSAEQLRQIEPLVRAVEDEDRLYWGIRGERAMLVGAVTWMTASG
                     ARGIPGAASLPLPANIPGVRGWVMGDMAEGLRLENRLVKAKGPRERIKAATEVETAVV
                     RLPRYRVLSRTLIPSMKRAFSLDLHVTAMARSTRAALAAECYRLDKGRFPDKLEELVP
                     GYLDAVPTDPFDDKPLRYRVDPDGVVIYSIAEDRNDGGGQVQGRMPPKSRPLDVGFVL
                     LKPDSRGRPASSTAPATQEAEATAVSTGSAR"
     gene            complement(21085..>22094)
                     /locus_tag="P5316_08860"
     CDS             complement(21085..>22094)
                     /locus_tag="P5316_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR85107.1"
                     /translation="AGSAQASFDVPGAVCFVDADTCIVCGAAVNHVSGYGIEVGTGCE
                     NICINNCEITDLGAGGVKVWSGSSHTIVHNCEIGPGGLIFHSAVGVLIGPSGDNTVSH
                     NHIHDFNYTGVSVGWSWGYNPSPAVRNIVEYNHIHDIGKGMLSDMGGIYTLGVSPNTQ
                     IRYNLIHDIEAHTYGGWGIYNDEGSTHIHIENNIVYRTKHAGYHQHYGKENYLRNNIF
                     AFGREAQMQRSREEEHTSFIFERNIVLFDGPNLLAGNWKSDKFVTDYNLYWRTEGQPF
                     DFAGASFEDWRKRGHDTHSIIADPQFVDPANGDFSFKPGYPAHQIGFVPIDTSKIGRI
                     K"
BASE COUNT         4295 a         6769 c         6619 g         4411 t
ORIGIN      
        1 ctggatgtgt cgctgaccac gaccagcgga tgcttcggaa ctcgcagagg accgcaaaat
       61 gcagtcacga ctctcgaaca taaacggccc tgacgctatc cccaaggcga ttctgggctc
      121 acaagtgttc aacttgggct aacaaagcct tcgtttccaa tcccgcattt gccaatctcc
      181 atttcgcggc gttgcagtat gcatcgccgg ggttaatatg gctcggcttc ttgaggagga
      241 taataaacgt gatcgcggaa agaccgctgc gttggccgat ggccattctg ctgattctgc
      301 ccgccaccgc catcggcgcg ccggtgatga tcgaagcgat tcccggtcag gccgagttgg
      361 gcacggtcga gatccacggc gtctcggcag gcccgttcga tcctgccatc tggcgatccg
      421 gctcgctcaa cctggtgccg gacatcaggc atcctctcct ggctccttgt cagggtggcg
      481 ggttccgcaa catctacgct ccctcgccgg tcgaagtgcc cggcggatgg cggctgttct
      541 acggggcctg ggacggcgtc caatccggca acgaccgaat ctacagccgg ttcaccgcgg
      601 acttcatcga cttcggcgaa cggcgtatcg agatcgagca cggcaccttc attcacgtgt
      661 gcaatgtcaa cgccgtccgc ctgcccgacg gttcctttcg tatggcgtgc acggcatatc
      721 cggataccaa cggccagaac aagccggcca tcttcaccag ccccgacggc agagcttgga
      781 acgacaaacc ggcaccctat ccggcgaccg cgagcgatat cattcagatc gaaggctatc
      841 ccaactacga cgcggccgac atcaacggca tgaacgtgct gcgacacgaa aacggcaagt
      901 accgcctcta tttcggtgac ttcaaggccc gcctgcaggt tcacagggca tcatccgacg
      961 acggccgccg cttcacgtac gacggcgtca gcctcgatgc cgccatgatg gtcaacgacg
     1021 tccgcgactt catcgttgat ggtcagcctg tctatctgat ggcccttcac gccaacacca
     1081 gccgactctg gtacgcgctc tccggcgacg ggatggaatt cgagccgcaa cgcgagatgg
     1141 ccgtcagccg cagcgacgcc gacaagtaca tcgtcgcggt cggatgggtc agcaacggcg
     1201 accggcttct cgggtttgtc tatggcgctg gggccgtgcc ctcgctcgac cgcaaccgcc
     1261 tcttcgcccg ctggctgcaa aagaagctcg tattcacaga ccgacaaggc aacaccctca
     1321 ctgtcgctgg tgctctcgga ccggaccggc agatcatctc cattccggcc cgcgagacag
     1381 agggacgcct tgagatcctc tccgaagacg gcaaaacccg cctgggcgag ccgctcgacc
     1441 tcaagctgac ggccggaggc atgtaccggc tcacatggag ggtggcgcgc taacgattcg
     1501 ccgaggatct tgcgttcact cgctccccga tcaggtcaac aaatcggaac caccggcccg
     1561 agaaagagcc ccggagcacc cgtgtgatga cgaggcttcg gggccctttc aagaaaagac
     1621 ggaaccgtaa gatcaagcgg cgcgggtgac gacgaactcg ccgtattcgt caccgcattc
     1681 gatccccttg gtctgctggc aggtgaatgt ccgcatgccg gtcttgccgg tcggatcgta
     1741 tttgccaccg taagccagat ccatgaacgc ggcgcagacg ccgcggatca tgtaggcact
     1801 gggcttcgct gaagccccgt aggaaacgac gtcctgctcg tagtagtcgt aggcccgaac
     1861 gaccattttt tcgttcggca ccagctcgac aatctcgctc ttggcccaac cccatgcggt
     1921 gaacacggcg aatgcaccat gcagaacgtc ctcggtattc tccaccatcg gggctaccac
     1981 tgacttccac tcttccgagg tgatgatgcc gtgaccggtg tggtaaccac attcgtgggc
     2041 agcgttgatc agcagccact cggcggcttc cagcgtatcg ggaccgacgc cgcgagtcag
     2101 ccgttcgccg aatccgttcc agaacttcgt gggaagctgg ttgaccagca cgccgaatgc
     2161 cggaatcagt ccttggtcat tgcctacgac ctggatcttc gatagagatt gaatgactcg
     2221 tttacgatca atagccatgc taaggtctcc tttcactcag ttcgcaacga cctggaaccg
     2281 cgaactcttg gcaccacaca cgatgctctg cgtctcaacc gcgttgaagt gccgcgtcgg
     2341 ctgaccgaac acggccgaaa acaggccggc aatgtaaccc gacgtgacgt agttcaccga
     2401 ggtctcgtgt ttgccccact tcttgagcca accctcgtcc acatgcgagt ggaccagctc
     2461 gacctcaccg ccatcggcac ccgcgcacag aaccttcatc tgaccaaggc cgatcgccga
     2521 gtagtactgc tcggcaattg tgatccggtc cgtcacgtcg gtgacttcgt gctcccggta
     2581 gtatttgttg agcatctcgt tgaaggtgtc ctcggcgacc tcggccatga gcttcttgcc
     2641 gtcaagcatg ccgcagtcct ccgccagttg cgtgtacaac gtgatgaagt ggtggcagtg
     2701 catgaccatc agctcgccgt taaccatgtg ccggcaggtt tgcggatcga acttgtgatc
     2761 cagaatcagt tgtgtttctt ccattactgg catgagcgcc tcccctttct atgcgatagg
     2821 tgcatcctta ccggtgatct tcttgaccgc atccttaaac ttcgcgatgt tctccggcga
     2881 atccggcgct tcgccggctt tgtttgacgg tatggccgtc atcatcgcca accgcatctt
     2941 ggcttcgagt ccgccgttgg cgccgacgaa ctcatagaac gccgtcagct tttgtgccat
     3001 aagtaccccc tttgttctct tttatatggc ctgtcattac gtatcacccg agcctctgaa
     3061 ggcttcggaa cctcgatcaa acaactgttc aaactctttt cttgccacct ctctttcgtc
     3121 acgagggtat atggcgcggc attcgcagtc gccctcaacc cccttgatct gacaaccgca
     3181 ctcagtccgc agcctgaaaa acggtcgcgg aaactcggag aagtagtcaa ccggtcccag
     3241 cgttcgaagg agttggatcg tttcgggagt ccacggaacg tcgaacacgt acccgtaaac
     3301 cccgcttccg tcaaagcaat cttcgatctt gcggaccgca ataattctca agctgccaat
     3361 cccctgaccg catcaagcgc cacagcaacc tcctcttcac tcgtgaatgc ggacgtgctg
     3421 aagcgaaccg tcccctctgg atcactgccg atcgcccgat gaataagagg agcacagtgg
     3481 agccccgttc gacaggcgat gccatagcat tcctgtagga cgtaacccat ttcctcgacc
     3541 gtccacccct tcacaacaaa cgagacaacg ccgatctgct gagcgccgct tttgccgtca
     3601 aaaaccttca cgttcggaat ctccaacagt ccctttcgca gcgtgaccgc caccctctcg
     3661 gcacgttcgc gagcgccgga atccaggccg gtcgcccaat ccaatgcggc cgcaaacccc
     3721 gccgctgccg gcacgttcgg cgtacccgct tccagccgcg cgggcatgtc ttccggttga
     3781 tacggcaaat cgctcctgac acccgtccca cccacgatca gcggctccag gtcgattccc
     3841 tctgcgacgt acaacacacc aatgcccggc ggtccatgaa aacctttgtg cgccggaaag
     3901 gccgtcaagt ccgcgtgcag gtcatccggg ttaaccggaa tgagtccggc cgtttgggag
     3961 gcatccagca tggtaacggc gcctacagcc ttggccttcg aaaaccagtg gctcacgtcg
     4021 ttgatgcgtc ctgtcacgtt tgatgcatga ttgaacacca ccaacgccgg ttcgcgctca
     4081 agctccttgc tgattgtctc ggcgtccaag gctccatctt cggcaaggcc cacaatgctc
     4141 aaacgaatac ctgtggtcct gaccaagtgg ttcagcggac gcaagacgga attgtgctcc
     4201 gttaccgttg tcaccacgag agagcctcgc ttcagtctga ggccgctgat tcccatgttc
     4261 agcgcctgtg ttgctcccgc ggtcagtacg attcgactcg cttttctgac tcccaggtac
     4321 gcagcacacc tcatgcggca atcggttaag acgtcttcag cactcgcagt cgcccgcccg
     4381 gggtgctccg gcgattggga taagacgcga acaacggctt cgatcacgcc tggtgccttg
     4441 gggtagccgg acgctgcgtt gttcagatag gcgcgccaaa caccccttcg tgccaccgca
     4501 acaccaatga cacccatttg tgcgtctgca gacttcacgc aacccccagc gaagcgtttc
     4561 agtcggatgt cccatatgga gaccatctat gctgcctaag tcagatcccg tgtcggtttc
     4621 attgctacaa accttgaggt ttctgcggct ttgccggcgc cgttttggcg ggttttcgcc
     4681 attacaggcg ttagatactt ctaaagattc acacacacgc gaatcaggct gttttcagac
     4741 cctttggctg acctttgggt gcgaatgaga tccctatcag atgggcatac cgtagaatgg
     4801 ttgacagttt atgtttgttc ctgtgacagc aatagtttgc gaagtcggaa cttatgttcc
     4861 caaccgggtc cgaaggcgct agctctctcc tgctggcgaa gcgcaaagac ctcgtccgct
     4921 acaacaatga ctagaatgca tcacaggtca gcttgtaaga gcgtatcgcg caccgcaaaa
     4981 ccgtcccaaa gatgcgagac ggttcttcgc cttcatccta tcgcgattgc ttggcaggtt
     5041 tatgtgcgga aaacgatatg tctttttgcc caagggtatt gcgttgtctc gcgcattcgc
     5101 gcacaatgcg gcaacgaacg gtgtttgctc ggcgcggcga acccggctcg tgacagctta
     5161 gcaacacaat tgtggaaagt cgcatgctct tcagtcctga tcagaggaag ggcgcgcccc
     5221 ttcgaaattg aggagaatag aacatggcga gaacgcttct ggcatcatta gtagtcctgt
     5281 ttgtcgtccc aatggtcatc gcgcaaccca ccactgctcc ggccggctac ggcggaagtt
     5341 ggaccgagtg gtccaaggct accggcgatt ggggcggcgc ccgtaccgcc ctggctgaca
     5401 agggaattac cctggattgg ggcatcaccc agatcgtgca ggaaaacatg cacggtggag
     5461 cggatacgaa cagtgccatc aagtattctg gatcgagtag ccttgagttg aaactcgata
     5521 cgggcaagat gggtctttgg ccgggcggaa cgtttgtctt tcacggcgag ccgcggtggg
     5581 gccgtgggat caaccgcttt gtcggcagtc tgatgcccgc caacatggat gctctcatgc
     5641 cgggcggcga cgacagttgc gagatgactc tttcagagtt tttctaccaa caagttctgt
     5701 tcgaaggcaa gctggtcttg ctggccggga aacttgatgg ttcccgagcc ttcgacacca
     5761 acatgttcgc gaacaacgaa gaaacgcagt tcatgaacgt ggcgctacgc aactatccca
     5821 tgattccgca gtttattcct tacacgacgc tggggatcgg catcgtcttc aacccgttcg
     5881 aatggtggtc cattcgaacg gcggtggtcg attcagacgg cagtgccacc cggacggggt
     5941 ttgataccgc cttccatggt gacgccaaca ccagcgtcat gcacgaatgg gatttcaccg
     6001 tgaagccttt cggccagctt ggtcaccagc gggtggcgat gatcacgagt tgcaaggaca
     6061 cccccacata tacgggcgga atgcagaact gcaaccttgc ggtcttgtac aacttcgacc
     6121 agtacctctg gaccgagtcg gagggctcgg atcagggcgt gggtatcttc ggccggtttg
     6181 gatgggccga caaggatgtc aaccccgtcg agtatttcta cagtctcggc gtcagcgcca
     6241 aaggtctgat tcccacgcgg gatcgcgata cttgcggcct cggctggtat ttcggcaacg
     6301 ccagcaacca gctcgcgcct gccgggattg tgcaggaaag cggtatcgag atgtactata
     6361 gcatcgaggt cttcccctgg ctgcatatca gccccgattt ccagatcatt tgcaacccgg
     6421 gtggcggcaa cgcggacacg gccctggtgg gcggcgtccg attccagatg gatctgtaac
     6481 agaaccggcg ataagggtcg actcaagcat tctgtggacg gcatcggcca ttggctgatg
     6541 ccgtcccgct ttttgaaagg ggtcgagtct cgagcatctg gttgcgacga agattctcat
     6601 caaccggacg ggccttcaag aacggcagcg cgacgctcgt cgcccgctct caacataaaa
     6661 tgccccctat tgcactctgg aaaacccgcc tcggccgggt ttcctcgcct tggccgacca
     6721 ggcaacccaa caatcaatgc tggcacgcat gttgcctgat gcaaccggag aggagcaagc
     6781 aggcggctca agagcagaag acgaggaggt attcctaccg ggccgtaggt tcccaatcgc
     6841 gaatcgtacc tgccggtagg gctaccacct cctgtttcgc agtgaacggt cagttgtgac
     6901 cgtcgaacac ggaaaggcgt cgaggagaca cgttcatgaa ctcaaacgac atacggctgg
     6961 ttctgtcggt tttggtcctg ttgtcggcaa tcggcggtct tcagatggcg gtggccgccg
     7021 atccggaggc gatccgcatg gatccggcgc agctcgtttc cgacaccctc gaacctgcgg
     7081 tcgcacgccc gctggggggc ggcgaacagg gaattcccga gacccagccg gcctctgcgg
     7141 actggcctcc cggtctgatg atggacggct tggacgccat ctcactgggc aggccgatga
     7201 gagacctggg cctgcggctt tacggccacg tggaagccgg aatgaccttt gagttgaccg
     7261 gcccgggaga caagaacacc ggtctccccc tgcgcggctt cgattcccgc cggccgcaga
     7321 acctgcgcct caaccagttg atgctctccc tcgaccgtcc tgtggatacg agcaagagct
     7381 tcgacgtggg cgggacgttc actttccttt atggcaccga tgccagactg acccattcat
     7441 tggggttgct tgacaagcag aaccacgagg ctcaaccgga catcttgcag ggttatgggg
     7501 agttgtggtt caagaccgga gacgcggggc agggcctcga tgtcatattc ggcaaatggt
     7561 ttacacccgc cggctttgaa gtgactcccg cacccgctaa ttatctttat tctcgtggtt
     7621 acctgttcac atttgcccaa ccggtgtcac atacgggact gaaactcacc tacttcttcg
     7681 acccggacaa ctacgcctac ttcggggtgg tgcgcggatg ggatgtcttt gacgacaaca
     7741 acgacggtgc aaccttcatg ggggggtttc agttgtccag caagcagctc atgggcgaca
     7801 accctcgctc gcaactggcg gtgaatgtca tcgccgggcc cgagcagacc ggagagggct
     7861 ggccaggcaa ccgggtgctc gtggacgtcg tctggacctg gaggtggacg gagaagctga
     7921 cccaacacat caatgcggat tacggattcc aggatgacgt cccggaggcc ttggggaacc
     7981 gcgacgtgcc gggcgtgcgc gactcggcat ggtatgggct ggggtattgg ttgaattaca
     8041 cgttcaacga ctacctcagc gccactggtc gggccgagtg gttcaccgac aaccatggcg
     8101 taaggaccgg ttatcgcggc acgttcttcg agctgaccac cggcctgagc atcaccccgt
     8161 tccccaatga tcgtatcctg aagaatctga gcatccgccc ggagatccgg gccgactggt
     8221 cggcaaacga cgcaccattt gccgagagtt gccagatgac cgctgggttc gacgtggtct
     8281 acaagttctg acccatatgg caggacgatc gccgctcccg ccagatttgc cttgaatcag
     8341 acgggcagaa ataacgatac ataacacagg cagattgccc gcagcctgcc gccgggcagg
     8401 gaggacgtct gacccgccct tgacgcgccg ccggccggcc tttcaatact gggaagcggg
     8461 tttctgccac cagcggattt cgcaacctgc tctggcaagg aggagcccat gaatgatcgt
     8521 cccatcgggg tgtttgattc cggtctgggc ggactgaccg tcgtccgggc gctgcaggaa
     8581 cagctcccca acgaaaccgt catctacttc ggcgacaccg cccgggtgcc atacggaacc
     8641 aagtcgtccg agaccgttct gaccttcgcc gagcaggatt gcagttttct actgcggttc
     8701 gacccgaagc tggtcatcgt ggcctgcaac acggccagcg ccctggccct cgactacctt
     8761 aaagaaaaaa tgcccgtccc ggttctgggc gtggtggagc ccggagccgc agaggccgtt
     8821 cgcagatgcc agggacgcgg catcgccgtc ttagccaccg aggccaccgt ggcatccaat
     8881 gcctacgcca aggccattgc cgccttggcc cccaacatcc cggtcgcaca gcaacgatgc
     8941 cccagcctgg tggccctgat cgaagagggc cgcacctggg acgaccccat catgaccatg
     9001 ctcgtagccg agtacctcga gggcgtcaaa gatcttgacc ccgccgtggt cgtcctcggc
     9061 tgcacgcact atcctcttgt ggcaaaagca gttgcgaaac agatgcccca tgccactgtg
     9121 gtcgattcgg ccggcgctac cgccttggcc gcccgtgacc tgctggttcg cgaggaggct
     9181 atgagtgatg cgtcagcccc cgggcgtctg cggtgttacg tcagcgacaa ccctcagcgc
     9241 ttccgcgaag tcggcggccg tttcctgaac aaacccatcc gggacgtgac ttcggtcacg
     9301 ccggaacagt tttttctcga acaggtcctg gagactgcgg ggtcaatgac gtgagccggt
     9361 gaagcagtct cgtaccggcg cttcggggac agcatccggg ccgcgcaatc gcgtattcca
     9421 gcactgcgag caacgaacgt cgtctccgtc ggggcgagca agccgcccat ggccccctgg
     9481 gtggacgaga gtggcattcc ccttcggcct gaaagggcat tctcgccaca acattcccgc
     9541 cgtctgccga atctcaagaa cgttcctgct cagattcgtt tgaaaaacgt ggggcgggat
     9601 tcagcggcgg gaggatctcc tcgatcatct tggcccagac cttgtacccc gcctcgttga
     9661 ggtgcaggcc gtcacgcgaa tactccttgc gaagcaggtt ctgctcatcg gccagcggct
     9721 tgaagacgtc catgaacgag cagttatggt ccgcggcaat cttggccagc cgcttgttga
     9781 attcccggat gccgggtgtg agctggtcga accggtcgcg ggtgggaaac agtccgacga
     9841 tcaccagcgg cacgtccggc agccggtcac ggatcgtctt gaccaccttc cggtagcacg
     9901 cgtcgatttc ctcgatagtt ggtgtgccgt ttcgctgcag ttcgccaaga tcgttcacac
     9961 cgttttccag aatgatgaaa ccgggattgc agtcgaatac ggaactgtcg aggcggtgga
    10021 ggatgccgcg atcggtaagg ccgatccggt ccgaggcaat accgcggttg accactcgac
    10081 ggccgggaag cagcctggcc gcatcaaagc cttcgatgtg cgacgatccc accatgacga
    10141 tgtttttcgc tgcggcgttt tccttcttga ataactcaac gcggtcatgg taatgcttga
    10201 cccacttgtc ttgagcggcc gccggccggg tttcaggaag ggccgcgcga accgtaatca
    10261 ccagcccgta tgatgcgccg agcgacagaa gaacgatcgc caggcagcgc ctgtgatgct
    10321 tctctcggat catgaatcgg gttcctcctt caacgatacg tggtgttgtt ctctcacgcg
    10381 attcaacagc agggtcaaca ccgcgcccac cagcagcgag gtcgcggcca caaatagacc
    10441 ggccttgtag ttccttgttt ggtcgacggt caccccgatg agatacgggc ccacaaatcc
    10501 gcccaggttg ccgatggagt tgatcatcgc gatgccggcg gcggccgccg ttccggccag
    10561 aaaggccggc ggcattgccc agaacggacc gagcgtgctc cagatcgccg atgcggccaa
    10621 agccattgcc acaatgcccg ccaccggctg atcggtcatc gcacacagca tgaagccaag
    10681 cgctccgccg cccgcgcaga gggccacgtg ccggcgtcgc tccccgaagc ggtcagaatg
    10741 tcgcccgatg agcaccatgg cgatcatcgc tgtgaaatac gggacggacg caatcaggcc
    10801 gacgtggaga ttgtcgcgag aggcggtgac ctgtttgacg atggtgggca gccagaagtt
    10861 gatgccctgg aagccgaaca tcagggtgaa atagatgatg ttgagcatca gaaacgttgg
    10921 actgcggaac gccctgaaga acgaggtgtg accgatgctc ttgagcctct cgcggtcggc
    10981 tcggacttga ctgttgagcc aacgctgctg ttccggactg agccacttga cctgttcggg
    11041 acggttgggc agcagcagca ggatggcgaa cccgccaagg acgctcggga tgccctccag
    11101 cagaaacagc cactgccagc cggccaacgc gcggcccaag acggcagtcc cgtccaactt
    11161 gagcagcaac ccggccagcg gcgaaccaac ggccccggcg atggccgtcg aagtcagaaa
    11221 ccatgccacc gccttggcct gatgtcggac cggcaaccaa taggtcagat acagcagaat
    11281 acccggggca aacccggcct cggccgcccc aagcaggaat cggagcgtgt agaagctgta
    11341 cgggccgcgt acaaacatca tggccgacga gatcaatccc catgtgatca tgattcgagc
    11401 cagccacagc ctcgccccga cgcgttgcat gatcaggttg ctgggtatct cgaacaggaa
    11461 atagctgata aagaagatgc ccgcgcccag accatatacg ctttcgctga agcccaggtc
    11521 gtccttcatt tccagtttgg cgaaggagac gttgacgcga tcgagatagt tgaacacgta
    11581 gaggatgacg atgaagggaa tcagccgcag caggaccgtt ctgacggtcc cggtctcgca
    11641 gttggacgcg cggtttgata cgcaggccag gggcttcatt ggacgcgcgg tagcataaca
    11701 agatgtgcct cggttgagaa ccgcgcaccg ggaaccactc ggcgtggttc cgagccgatc
    11761 gcaccaggcc tgcaagaggg cggccttact gcttggcaga gctcttcgcc cgggcttctg
    11821 cggtgcgtat aagtgccgcg gaagctgcct tgataccgtt cacgtcgttg agcatgcaat
    11881 tgaggcccgc ggcggtgagc acgcggccgg caggcaactg gacctgcttt tcaaatccgc
    11941 tttgcaggtc cacgccgccg cggaagagct tttcttcacc aagcgtgcca tccttgttga
    12001 gcggcctggc ccaaacgccc atccgcttga cgtcccactc aggagcaatg ccggcgccaa
    12061 aacccgtggc gacgtaaccg gccggaagct cgatctgtgc ctcaaggccg gcgtcacgtt
    12121 cccaaccggt tcgcagttcc tccggctcgc ccagcgaccc gtcgggcatg agcggctgaa
    12181 tcttcatcca catggttgtg atgttgtcgt agtgtgcgcg ggcccccagg ccggtgatga
    12241 cgtatcccgg tggtgcctga atgaaccctt ccaccctgtc atctgcctcg atcttgtggt
    12301 cgatcaatac caacgacagg cgttcggcag tccggacggg cggcgcgtcg gccgccgcag
    12361 gcgcggggcc gagatcgtac acgtggacgt gatcgacgta cgcactggcc gcatcaagat
    12421 cgcgccagcg gaagaatccg aagccgatcg tggcctgctc ggcggttgcg atccaacgat
    12481 gctcgaatcg tatccacttg ccgtccgtcc agtaccattg gctggaattc ggcccgtcga
    12541 agttgtcccc gccgtcggga tcgacaaaga gcctcacccg cgtatcgcca cgaggcccgt
    12601 tcttgacggc ggtgaacgcc atggccgcca gtgcatacgc atggcccggt gtggtggcga
    12661 tcttctgcgc gagtgtgctc ttggcaaaaa cctgttcgcg tttctcgccc gccatgtgcg
    12721 tgaatccggc catgcggctt ccccaacgtg tgccgatgcc aagctgcttc agttcggacc
    12781 gactttgcac gcccacatcc ggcgagtcca gcgcattggg cggcgagacc tgccagccga
    12841 tgaggccctc ttcgaactcg gggttgacca gctccacggc tctacgatat cgcagcggct
    12901 ccggatcggt cccggcggcc acgcggcttg gcgcgaggtt gacaaaacag ggaggataag
    12961 gctccggcga cgacagcacg gcttcgatct cattggcgcg aggcatgcgg atccgataca
    13021 gcggtgttcg ccacacaagg cccgtcggtc ccgtggccgt caaccggaac tcgtaatcat
    13081 ggcctgacca gacgggaatc gtcgcctccg tccggccctt ctccacgtcc acgcgctgca
    13141 ggtcaggtcc ggctcccttc atgccggggc cgcggatctc gatccgcgtc tgcgttggga
    13201 agttctgcga ccaggtcaat ttgagcttgc tgtcctgggg tgtcgcggct gtgaggttga
    13261 agatcgcagg cagtgcgccc ggctgcggct cgccatagac cgtggtgtgc atgtcgcctc
    13321 tgatgatcac aacgtgatca tccttcggca gcggggcttt gtgggcaacg gcgtagactt
    13381 cgatttgata ccgctcgccg accgcgacag ggaactcatc cgcggcaaag aggaacggag
    13441 cagcgtgggg accgttttcc ggtccgcaac tcagtcctcg cttcgggccg gctacgatct
    13501 tgccgtcggc ggatcgcacc cggatgacca ggtcacagca attgtgttcg gtgggcgggt
    13561 tcatcaggat cggcgatacg atgagcgaaa ccattcgcac gttcggtgtt cgcggagtga
    13621 accagatacg gtcggtgtcg tacacccaac cttcctcatc ggtgttttca acgggcgaac
    13681 gtttcaggtc ggccggctct tcaacgatcc agacggccag gtcactctcc ggccgtggga
    13741 ggccgtcaac gtacaaaaca cccccgtcgt acgcgttgcc gatcgaatga aggtagggca
    13801 tccaggcctt gccatcctca cgccacaggc gaattccata cgtacgaccc ggcaccatcg
    13861 gggcctggcc ggtgatccag cgagcgatac cccattccat tgaatggccg gagacaaaag
    13921 tctttgtcgg tccgatctgc ctgccgccgg gccccccttc gactaaggcg gcgcgaaaga
    13981 tacccggctc ggaagccacc atcagcgtca tgctgaccag ctccgtcgag gtggcagtga
    14041 aggtttgcac gaactcgcgg gccgaccagc cccagacaca tccgtcaggt tggtacacat
    14101 ggtcgatgac cagatcggcg ctgttgattc ggctctcgtc ggcctgggcc cccgagatga
    14161 tccgcgggta gatgggacca tgttcgacga acgtgaagtc catcagccca tagcagaaag
    14221 ggaactcggt gtaaccctca tcggccatgc ccgtattgcc gatctcgacc cgcttgtctt
    14281 gcgtccagac cgatcgggtc ttcggcagga tcggggcgtt gtttcgtgaa cccaccattc
    14341 gatagaaaag ctgacggcct cgcggtttgc cctctccttc cgccttctgg ccgaccaatg
    14401 tccccggtgc taccaggcat acaagcccga tgacgataag cccgcgagtc gtcatgatcg
    14461 ggtttccctt ctcgtccaag cggacgccgc cggacaaccg gcgttggctg ggtcttttga
    14521 gtcttcgtgt ccaactgggt tcagcgcgaa tccgccgagc aaggatagaa ggtcgggacc
    14581 cgcgcgtcaa acccctgatc tatggtgata catcgctggc gcagggggga gtgtggggat
    14641 gtcactcaga gcaaagccta gggccttgcc ttgccaggtg tcgcgacggg caagagcccg
    14701 cttgatgtcc tccaaaacgg cggctttatc aaggcaccac tccggggcaa cgagaatgtc
    14761 gcgcggggca tcggcatgca tgcgctggat gaccatcgtg gccggcagtg tttcgagtac
    14821 atcacacacc agatccacgt actcctgacg accgagcagc gcaagctcgc cgcgagcata
    14881 ttgagcggcc atgaccgtgt cgcgcatgac gtggagcaga tggattttga tgccgtcgat
    14941 gggcagttgg gcgagtcgac ggtgagtgtc gagaatcatc tcacgcgttt caccgggcag
    15001 cccgagcacc gtgtgaacgc agatcttgat gcctcggtcg cgggtcatct caacggcgtc
    15061 cagaaactcc tggtaaccgt ggccgcgatt gatcgctttg agcgtggcgt catgggcgct
    15121 ctggaggccg tattcgagcc aaacctcgcc gcggccggag tatccggcga tcaggtcgat
    15181 cttggccggg tcaacgcagt ccgggcgggt gccgatcgac aacccgacga tctccgggaa
    15241 cttccacgcc tcatcgtaca gctctctgag gtgctcgagc ggtgcatagg tattcgtgta
    15301 ggcctgaaag taggcgatga acttcgtggc cctggaccgc cgacgggtct gctcgattcc
    15361 ccattggagc tgctcgcgaa cgacggccgg gtcggtgcgg gcattggggc tgaaaccggc
    15421 gttgttgcag aacgtgcagc cgccgtagcc ccgtgtcccg tcgcgattcg gacacgtgaa
    15481 ccccgcgtga atggtcacct tgtggacccg gcagccgtaa cgccggcgca ggtagcgact
    15541 gaacggatag tagcgaggcg aatcggtcat ttttcgttca tcaacccggt tacaggcctg
    15601 gccggccgcg caaggcaacc gaggggttta ttgctctccg gttccaagcg cggggcctcc
    15661 tgtgccgatt ttcggctgac catagccgca caacgtctta tgatagcctg tgtgcgacgt
    15721 tctgtcattg tagagacgtc tccgcgcatg atacgtcgta ccctgggagg ggctttgggc
    15781 gatgatcggg gcgttgtccc gcgcacgatg gttgatctac gtgggcatat gcttgccttc
    15841 ataccctcct gtgctcgtcc cgtgagggct gggttgcgcg cgacgctctt ctgccgggga
    15901 tgggagtcga tgcagaaagc gtctctcccc agttgcgccg atctctcttc gacaatcctc
    15961 gcccttttcc acctcacggt atccttgtca gtgtaatctc cgcctccgcc gtctgaccgg
    16021 cggcaggagg atcaactgga aggaagccgg gcaaagggga atcgagcctg attctggcac
    16081 tgaccgtgac cgtccctcgc caggaggctc cgatcggcga aaccagtcgg accgagccgg
    16141 tcagcatccg ccggatcgcg gcagcgtcca acgccatgcc ggtggcgacg caccagcggt
    16201 cgatggggat ctcaaacgtc cttgtttgcc tcggctccgt gtggaactcg gctctgtcga
    16261 gcttcatgaa gaaccacgtg ccgatctgct cgatgctcac gacaaacggt gcggccgtgt
    16321 cgttgatggt cacggagaat cggcccgtcg cggcaggata cggccggcag aacctgttca
    16381 atcgggaaat cttctcttcc cacggggctt tttccgctag ttgattcagc acttcgctct
    16441 cgggcttcaa accgctgaga atgcatagac cctcggccgc cgccatacgc acgttggcct
    16501 cttcgtggtt gctcaagacc gccagtcggc gagggttgac tgcttccagc accgccgtgc
    16561 cttgctcgcg accggcggcg ctgcccgcca tgtccagaat cgggcggagg ttggtcacat
    16621 tcccgcagcc gatgagcagc accgccgctt gccggatgtc ctggcgtccg gctgagagca
    16681 tgtcgagaaa aaccggaagg ttgccatcat cgacccgtcc ggcaaactcg gccgccagcg
    16741 aggactcatc gccgcccttg cgcaggtgct cagcggccag tcgggtcgtg gccatgtgcg
    16801 cggccttttc gccactgtcc agtgccgagc tggtggcggt cttgaacagc cggtatccgc
    16861 caatgctcaa catcgccagg gccgcgatgc ccgcaatcag cttccacggc agggccgaca
    16921 ggaccgacgg cttgacttcc accggccgcc gtggcggacg ccggacgatg ccggtcactt
    16981 gattcgcccc gcattccggg caggtgattg cctcgtcgtc cagcggtgcc tggcagatga
    17041 tgcagttacg gagttccttc ttctctttcg gagcctcgac gccgatccag gagtcctcgc
    17101 cgaggtcggt ctgcttccac tcgaacggca gccgcacctg gccgatgcga aaagtctgat
    17161 ggcagccggt gcacacggcc gtcttgccca tcccctcggt tcgcgttcgg taccgccgct
    17221 gacaatgtgg acaggcgacg cgaatcatga ttcacctcgg cctcaagcat ttcagaaccc
    17281 ggagcgcaag cgacgggcca accttctcaa tgccgccaag actcccacaa ccgattataa
    17341 tcccggtccg gaatcataat ggccccaacc aacaacatgt tgtaagaaag aagcggcggc
    17401 atgacaaggg cggcgattgt ggattggcgc ttgggccctg gggactggcg attgcgcccc
    17461 gcaatgggat tgggcacccc cggcccccac ccgccgatcc ccagtcccgg ccatttggcc
    17521 cgctcgccca ggacactgga aaggaccaag ccgtgacaga gggcattcag cagaagaagc
    17581 gttggccgtg gtggctccgg ctgattctct atctcagccc tctctggatc attctcgcga
    17641 tcatcctgag cgtcgagatc tccgggcggc gggcgtacaa ccgggcgata caggaagccc
    17701 gggacatcgg cgggccggtg acgatcgagg agatcgaggc ggctcgcaag gtctggccgg
    17761 atgatgagaa tggggccctg gttcttcttc gggttcgcga ccgactcgat gctattggca
    17821 aagaggccgc agccacaacg ctcccagttc tgggcaccct caaagacgaa ctcggatacc
    17881 gctggtcaga tgacaccatt caggctgtgg aggcatttct tcagcaacac gcagcggtgt
    17941 tcgccgagat cgaccgcctg cgacagtatg acggcgggcg cattccgttc gagatgaaac
    18001 cgaacccgct cgatacctgc ctgcctgatc gggctttcct tcggcactcg gccaagctgg
    18061 tgttcctgag actggagtct cggatcattc tgggcgatac ttcgcgagca gtggatgaca
    18121 ttgatcttct cttccgtcac gcacgtcttc tggatggtga agggacgctg atatcgagcc
    18181 tcgtcaggct ggccatcggc gctcttgccg agaatggagt ccagcgagtc ctatccttgt
    18241 gctcgctgag tgatcagcag ttgcagcagc ttgatgagat gctcgccgcg tttgagcgca
    18301 cggattgtct ggaaatcgga taccgcggcg agcgggcctt ctttctcatg ctcacagagg
    18361 aggcgaacct tcgtcgcgcc gtggccacag ggaacaaccc aaaattcgca acgctcccta
    18421 tggagctgcc tgtactgcat ggcgtgtttc agatggaccg ggctgccggt ttgcggatgc
    18481 tcaatcggat cgtgaaatcg aacacgctcc aggatcgtct caaggccatg atggagaatg
    18541 aggcagaaaa ggcatctgtg ccacgccttt acctggcgac gcggataatg atcccttctc
    18601 tcgaacgttc cgtggtcctg gaccagaacc gcatagctca agtgcgcgct gccagaaccg
    18661 gcctggcgat tgagcgattt cgctccgacc atggtcgttt tccacagcgg ttggaagaac
    18721 ttgtgccaca gtacctgcct gctttgttga tcgacccgtt cgacgacaaa ccgctgcgat
    18781 accgggtgac cgacgacgcg gtgatcatct acagcatcgg cgaggacggc atcgacgacg
    18841 gcgggtatgt gggccaccgg acagcgcccg aaacgtcgcc aacagactgg ggtttcgtcc
    18901 tgctcaagcc cgactttcgt ggccgaccgg ccagtaccac ggcaccggcg acacaagagg
    18961 ctgagactgc tgcggtctca acgggtccgg gcggagtagg gcatgcccgc gccgagtcgc
    19021 gggaatgcca tccggcgatg cagacgggcg aatcgggcca tacatccgct ttgcaccagc
    19081 gtcattatac ggggagccgg gagactggcc gccgcgagca acggcaacct tgcgatgctg
    19141 cacggttcca catcaccagt caccgatccc caatcgcggc agggggggcg gcacgatggt
    19201 ggcgtcggaa aggactgagc catgacagag actgataaaa acaagaagcg ctggccttgg
    19261 tggggccggc ttctgttgct gttgagtccg atcttcgttc ttctgctcgt gatcgtggtt
    19321 ttcgaggcag gcggccgcag gtccttcaac aaggccctgc aagacgcccg ggacatcggc
    19381 gggccggtga ctttcgaaga gatcgaggcg gcccgcaaag tcgtgccgga cgacgtcaac
    19441 ggcgcgttga tcatcacttc gctgaccggg ccgttgtcgc cgtcgacgga caaccctgca
    19501 catgagctcc tgccgcttct ggggcaggcc gaccttccac cgttgggaga gaagtggccg
    19561 gatcagactg accaggtggt ccgcgactac cttgcccgat tgtctgccga actgagcaag
    19621 atcgatgggc tggctcgctg cgaaagtggc gggatgcctt tccaggtggc acccaacccg
    19681 tttgataccc ttttgccgag cctgggccac gttcgcgaat catgcaagct gaagtcgctc
    19741 caggtgatca gcgaagcgat gcaggggaat acgaaatcgc tggtgaccga cctggacatt
    19801 cttctgcggc acggccagac gctggcggac catccgacgc tgatctcgag cttggtacat
    19861 gtcgcgaccg actcgttggc cctcgacgtc ctccagcgag ttctcgggca gacgacagtc
    19921 agcgccgagc agcttcggca gatcgagccg ctggtccggg ccgtcgagga tgaagaccgc
    19981 ttgtattggg gaatccgggg tgagcgggcg atgctcgtcg gcgcggtcac ttggatgacg
    20041 gcaagcgggg ctcgtggcat tcccggggcg gcttcgctgc cgttgcccgc taacattccg
    20101 ggcgtgagag gctgggtgat gggcgacatg gccgaggggc tgcgattgga gaaccgcctg
    20161 gtcaaggcaa aggggcctcg ggagcggatc aaggcggcca ccgaggttga gaccgcagtt
    20221 gtgaggcttc cgcgatatcg cgtgctgtcg aggacgctga tcccgtcgat gaagcgagca
    20281 ttcagcctgg acctgcacgt gacggccatg gcccgttcga cccgggcggc cttggccgcg
    20341 gaatgttacc ggctcgacaa gggtcggttc cctgacaagc tggaagagtt ggtgcccggt
    20401 tacctggacg cggtgccgac tgatccgttc gatgacaagc cgttgcgata tcgtgtcgat
    20461 ccagacggcg tggtcatcta ttcgatcgct gaagatagaa atgatggcgg tggccaggtt
    20521 caaggccgca tgcctcccaa gagccggccg cttgatgtgg gtttcgtact gctcaaaccc
    20581 gattctcgcg gccgtccggc cagtagcacc gcaccggcga cacaagaggc cgaggccact
    20641 gccgtttcaa cgggttcggc gaggtaagct acgcccgcga cgggccgaat gggatcttga
    20701 tgaccggctg aaagtgaatc tgtgtgcgcc acggtagcgt ctcatcagat tcatcacgaa
    20761 aacacgaaag aaggaaaaca cgaaagaggc cgctgggggc cccatccatc cagccgaagg
    20821 gtggatgggt ggggtctcgg tgaccgactg cgcaagaatc gtctggcagc gaacctggct
    20881 ttggtgattg atggggcgtt tgggctgatt cgatcatcgt attccccaac ctttgctggc
    20941 gcgaagggtg ggccatccgg ccatcttgat agcctcaagc cgccttggca gcatgacgaa
    21001 aggattcgtc gagggaatgg tgccagaaag gattcgggca ccagcagacc ctgaaccctg
    21061 aaccccgaat cccgaacccg taccctattt gatccttccg atcttgctcg tgtcgatggg
    21121 tacgaagccg atttggtgcg ccgggtagcc gggcttgaac gagaagtccc cgttggccgg
    21181 gtccacaaac tgcggatcgg cgatgatgga atgcgtgtcg tggccgcgtt ttctccagtc
    21241 ctcgaaagac gcacccgcga aatcaaacgg ttggccttcg gttcgccagt aaaggttgta
    21301 gtccgtcacg aacttgtcgc ttttccagtt gccggccagg aggttgggac cgtcgaagag
    21361 cacgatgttt cgctcgaaaa tgaacgaggt gtgctcctct tcccgtgacc gctgcatctg
    21421 ggcctcccgg ccgaaggcaa agatgttgtt gcggaggtag ttctccttgc catagtgctg
    21481 atgatagccg gcgtgcttgg tgcgatagac gatgttgttc tcgatgtgga tgtgcgtgct
    21541 gccttcgtcg ttgtagatgc cccagccgcc gtaggtgtgg gcttcaatgt cgtggatcag
    21601 gttatatcga atctgcgtat ttggcgagac cccgagggtg tagataccgc ccatgtcgct
    21661 gagcatgccc ttgccgatat cgtggatatg gttgtactcg acgatgttcc ggacggccgg
    21721 gctggggttg tagccccacg accagccgac ggaaacgccg gtgtagttga agtcgtggat
    21781 atggttgtgg ctgacggtgt tgtccccgct ggggccgatc agcacaccga cggcgctgtg
    21841 aaaaatgagc ccgccggggc cgatctcgca gttgtgaacg attgtgtggc tcgatccgct
    21901 ccagaccttg actccgcccg cgcccaagtc ggtgatctcg cagttgttga tacagatgtt
    21961 ctcgcacccg gtgccgacct cgatgccgta gccggagaca tggttgaccg cggcaccgca
    22021 gacgatgcag gtatcggcgt ctacgaagca gacagcgccc ggcacgtcga aggaggcctg
    22081 tgccgaaccg gctt
//
LOCUS       DAOTPW010000077        22089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2427_length_22089_cov_5.099936, whole genome shotgun sequence.
ACCESSION   DAOTPW010000077 DAOTPW010000000
VERSION     DAOTPW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22089
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2427_length_22089_cov_5.099936"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="P5316_08865"
     CDS             complement(<1..230)
                     /locus_tag="P5316_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85108.1"
                     /translation="MSAESNPCLTEKVIVVRVWTIVLLLILHADTPAGITVEFDHQQG
                     PAVTWHDEGGNVLFSMEPLVRWKGGRASTSART"
     gene            complement(241..1509)
                     /gene="purD"
                     /locus_tag="P5316_08870"
     CDS             complement(241..1509)
                     /gene="purD"
                     /locus_tag="P5316_08870"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRR85109.1"
                     /translation="MRVLLVGSGGREHALAWKLAQSKRVSKLYCAPGNAGTVDVAENV
                     PIKAEDVPGIVKFAREQKIDLVVVGPEDPLCMGLVDELTTARIRAFGPCKAAARLEGD
                     KAFAKEMMRHRSLPTAEARIFTKYRDAKAYVATRDAGVVVKAAGLAKGKGVIVCDDPA
                     AALIALERVMVAREFGDAGDTVVVEERLTGPEVSVLAFVDGHNIYVMENAQDHKPIGE
                     GDTGPNTGGMGAYSPTTLVDDKTLRQIEAEILVPIIDCLRTEGITYKGVLYAGLMLTT
                     GGPKVLEFNCRFGDPETQPLMMRLKTDLVDVFEAVIDERLDSITLDWDRRAAVCVVMA
                     SGGYPGDYQGGKPITGLAEANAIPDVVVFHAGTRMVGGQVVTAGGRVLGVTALGETFE
                     AARKRCYEAVAKIHFEGAYYRRDIGARARG"
     gene            1982..3232
                     /locus_tag="P5316_08875"
     CDS             1982..3232
                     /locus_tag="P5316_08875"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRR85110.1"
                     /translation="MFLTLATLATPTAPADPVFASWGDVFRAWGGIVRQFWLVALAAF
                     LVALIVTPIVRKMALSRGIVDQPDEFLKPHGHPIPYLGGVAIFAGWATAILLALWMTN
                     TMVTSWVMVGVALAGFATMLIGLFDDLRVMPPLVKLACNIGVAVILICLGIGDDLINV
                     VTRYSGMQFSQEDRWLELMYSVPVTIVIVVAACNATNLIDGLDGLCSGVLGIISIGFV
                     ILAVHLRVVYPSSPLGDDRVVLAMGMLGAALGFLPYNVNPAKIFMGDAGSMLLGFNAA
                     VLLLMFGEQRIVRWMIGATTIFGLPVADMLLAMARRWRNGRPIMIGDRSHYYDQLRDR
                     GLSVRRVAAISYVLALAFVIVGTSVIFIRTRYAVLVFMLTIGAVLAAIWRFNMAGIEK
                     ETPATHDRRKPFSPPPPANRRSTT"
     gene            3232..4062
                     /locus_tag="P5316_08880"
     CDS             3232..4062
                     /locus_tag="P5316_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85111.1"
                     /translation="MSQNHLEQRRNWFRFFPEGSLEFVVITRVLALLLMLMLMIVKGT
                     QAPLILLALLAMLWLDHMLTLSWTVQLATDLASFAEKPLPEADQEAVRFGPTLLILAL
                     PITLFFAAVAPWPAVFLPPGTPRETANAVLKPLLGLASVATLLPAFRRAKMLQIGRTF
                     WTFAYLLPLLHWVGIHRLLAPLDTRLLNQLRRSAASDEAGPGALAAAADVLWIISVLP
                     WLVMAALTFISLEPAASGGMAGRLACVCGTLLFGLYSVVDLAAMERVQRRFLRLIRSR
                     "
     gene            complement(4075..4677)
                     /locus_tag="P5316_08885"
     CDS             complement(4075..4677)
                     /locus_tag="P5316_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR85112.1"
                     /translation="MPRHGAQILLVDDEIEVQRVLARTLERAGFQVDAVGSFQEARRA
                     VDQRKYHLLLVDLSLPDQSGIDLIRDVRGRDESPSIIIITGHPSVRTATEAMQLGARN
                     YLTKPISPAALVEAVEFVLSSDGVLINSEEQFIAELGQRLKLARHETDLTMKALGERI
                     GISQAQISQIEAGLSAPSMTTLFRLARALRVKLADLFKGM"
     gene            4895..6217
                     /locus_tag="P5316_08890"
     CDS             4895..6217
                     /locus_tag="P5316_08890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013562946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRR85113.1"
                     /translation="MRLTVIGTGYVGLVTGACLADTGNEVVGVDKDAEKINTLTRGEC
                     PIYEPGLSELLQANTKAGRLRFTADIPKGIEHGDVVFIAVGTPPRDDGSSDMTAVREV
                     ADNIADHADRPKIVVLKSTVPIGTGDEIEERINRRAKHRVNVVNNPEFLKEGTAVEDF
                     LRPDRVVIGAEDPQAASVIRELHEPFVRNQRPIYIMHRKAAEMSKYAANSYLAARISF
                     INQIANVCDALGIDVNEVRLGMGSDRRIGFQFLYPGAGYGGSCFPKDVQSLVHTARKA
                     GVQADLLDSVHQVNERQREILFRKIVARFGGSLNNYCFAIWGVSFKPKTDDIREAPAV
                     TTINKLLEAGAAVRAHDPIALPNLRKLLGDRITYHESGYEALDGADALVIVTEWNEFR
                     SPTFEEIRRRLRQPIIFDGRNLYSLDAMRNRDFEYYSIGRPAVIPTRR"
     gene            complement(6405..8183)
                     /locus_tag="P5316_08895"
     CDS             complement(6405..8183)
                     /locus_tag="P5316_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85114.1"
                     /translation="MCRWFWGFCLVVAVAIPASGQTQYPIFTEDFESGLDAWTVLAYD
                     PTGGVNNNTPLVFDSTGAGIYGANIGYPGSQSVGFSSNLAPWDGQQPQWIEIQWPGLL
                     EPGIYDVVIVVDRYVYFDGDDPPNGPGDEDPWGVGNRVYVLTDSKYDNPVMNYDGDMG
                     IDGVRWSKWSNKGEGIWEIGAINRGQDIETTTGNVELRLLFHEKYAHYNTVAWDNLTL
                     TLRRPGGSVVFTYPAGTAGTLTTRTDADNGVVTLGAGHGLQPNDMVDVSWAGPAGSGG
                     IRRMKVSSVSENEVTVWAAVGAADALPAQGTAVVVNKPEDFEDGLAGWTTKLYGPKPN
                     DTPILFSATDPLLYDNVDNPGSQSAGFSSNMAEMDFTGAAWMQQQFPAAVPAGETYVA
                     TFEYDCYVYKEPGDPWGVGNRMYVLTDDQYNQPLWDFDNGDRGNGFSVDYWPGWLNNT
                     VDWSLNGHWQHVVTQQTISTTTGNIEVRLLQHDKHPGPQAVAWDNLSLTLTRVPPPCG
                     DNRFDRDGDGDVDQADFAALQMCFTGDGDPAFLFDAGACFCFDTNNDLDVDQTDLLAF
                     ENCASGPGVAADQACDDSLPPPVVPD"
     gene            complement(8656..11247)
                     /locus_tag="P5316_08900"
     CDS             complement(8656..11247)
                     /locus_tag="P5316_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRR85115.1"
                     /translation="MATAVSLLLTAVLWTRSAPASAPAAVVDGEIVRLIRDLNHPQPA
                     RRKAAAERLTELGDAAEEPLEKALPDQPAEARIAITGILKDIRLSRRGALVLLVFPRG
                     QAWYGGVRPGDVLLAVDGEAISDVEAWSKAEETRFRNRARTVRLRRDKRVYDRKFREG
                     KYGIQWCMYQSGWGDHLAEAAREHDVGNYAAAVAAIEKAIEAGNELGVDVEAVQGTLG
                     LITRCRYYALPPAERPAYLDMILKKPPYINHLAAIIGFATSLNPEDLHLHDEICRRII
                     RGGDMVEWAHDQLTYRLVFKERRYAEALVELLNPARAGEKRRPEDQAHALHKLATCLD
                     ALGADAELSVVLDRLAAFPQGRVRFGWLFPAAVRIGRLKLCEDVLPQIPEGSDAEGEQ
                     AYWEYIHAKDRLYQVYLRQGQVDDFRRALARGLADLGFAQDARLYSAWWPKTADLWAR
                     HAEEELKYAPAMNLDYLLSALAFQVDPNVAKLEKAIEFCEAAQRNRLTPSFHRARLEA
                     LKGNYAASLDWYEKIRAYDQENKPWNKPWDILPETEAVRFLSEHAKDLTGQDEKWRRV
                     LYAYKTDDGTRYLITRDIRIGKASPDGRVTEIPLPEPGWWPYRLADGLIVSDSGRTVL
                     AVSREQVYQLQPDAAGWSLLMHIPFSQRGWAIHKYAPWADELAAELRSHPPQARSIVL
                     PEEFPDHNNNAPIEPVMLGDGTWLYCEAKPHRMFNLSTMLARQLGRQVQIYSIFRSSR
                     AGVLYLGTERGLYRWVPGEDRCEPMPLPGQNPAPPVRIVVKQLPYPDDFQPFAAVLPS
                     AGGAMFLIDTKTQQVTPTPCVNELLSASYWSSRSPADRIAEAKATLVAARLDWDVLRL
                     AASRPVE"
     gene            complement(11466..12902)
                     /gene="argH"
                     /locus_tag="P5316_08905"
     CDS             complement(11466..12902)
                     /gene="argH"
                     /locus_tag="P5316_08905"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRR85116.1"
                     /translation="MKAQKSWQARFSQAADVLTENYVESLSFDRRLWKYDIAGSIAHA
                     EMLAEVGLIGKSDCRAIKRGLMEIASDIEAGKFKWDPACEDIHMAVEGALIAKVGEPA
                     KRLHTARSRNDQVALDLRLYTRDAIDLDVLPLLADLQAALVDSAERYADAVMPAYTHM
                     QRAQPISAGAYLLAYVEQFQRDRDRFSDARRRVDVCPLGSGALAGSTLPIDREAVAKR
                     LGFSSITRNSVDATADRDFIVEFLAAAALCACHLSRLAEDLVLYMSQEFGFVTIADAF
                     CTGSSMMPQKKNPDVMELVRGKTGRVYAALMGMLALTKGLPMAYNRDLQEDKIHLFSA
                     HDTLKASVEITAAVIRNTTFNTDRLLAATDRGFMDATILAEYLVAKGIPFRQAHHHVG
                     TLVAYAEKDNRELGGLTLDEFTKVCDRIGKDVYQYLGAANVVARYRSPGNAGPASVRE
                     QIGFWRKALSRAVNHLGNPSHSTCPRAE"
     gene            13096..14313
                     /locus_tag="P5316_08910"
     CDS             13096..14313
                     /locus_tag="P5316_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HRR85117.1"
                     /translation="MNRLWAWCLAAAWLAVAAGEAATTRGPVLERDPSQEALLAKQAG
                     PNFKVKRTSHFLIAYDTADEVARELDIRLEQTYHMVFLFCEMNGVQARWPDRRLEVIF
                     FDKRPTYERHVARLGVNAGGTFGIYHQQTNRSYFFNATNDEQVLRLKEQIAASNANIN
                     NLQQSLRNMPANGGIIEVRFSDGERIVGSKAEVEKRVNKKLNEARAELKKVEARQQRY
                     CDQINQTVIQHETAHQTLFNAGLHVRGAGNPKWLIEGLACLFETPPGPPGSGFSALNA
                     ARLEDFRVGVGGQTKRRLTSRDYETAIQTGRMVSPKELILNPELLNARGDAGLHNYAV
                     AWGLAHYLQRNQREQLAAYLLDVSSRLPGDDPTPQQELELFEEHFGPIDEIFLRKFSG
                     YILGLTARAQLDS"
     gene            14511..14801
                     /locus_tag="P5316_08915"
     CDS             14511..14801
                     /locus_tag="P5316_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85118.1"
                     /translation="MPATARHKALRLTLAAVLGGGTLLSPGCGDILRRSLRDGAIALI
                     AGTATGIIDPYDLSNLVTNMFTGGFTGGWNGIGSNWNWDWGWNTSPFTWPGT"
     gene            complement(15032..16030)
                     /locus_tag="P5316_08920"
     CDS             complement(15032..16030)
                     /locus_tag="P5316_08920"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRR85119.1"
                     /translation="MAKHVAVVGATGAVGQEFIRVLEQRSFPCDKITFLASARSAGKT
                     IKFRGADHTVQELTDASFKGVDLALFSAGASISRQFAPIAVKAGCVVVDNSSAFRMDP
                     DVPLVTPEVNPEDVRKHKGIIANPNCSTIIMVVPVFPLYKANRIKRLVISTYQAASGA
                     GYQAMLELEQQARDFLAGKPVTKKVLPHQIAFNLFSHNSKVGENGYNEEEMKMVKETR
                     KMFHDDEIQITATCVRVPIIRAHSEAINITFEKPMSEDRVREILARAPGVKLVDDRVR
                     NYFPMPIESSDQDDVFTGRIRQDISQPDGCGIDMFISGDQLRKGAALNAVQIAELL"
     gene            16353..16769
                     /gene="nikR"
                     /locus_tag="P5316_08925"
     CDS             16353..16769
                     /gene="nikR"
                     /locus_tag="P5316_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HRR85120.1"
                     /translation="MSELIRLSLSIEKPLYDRLEKLVKQSGYTNRSEYVRDMIRERLV
                     ETEWDKDREVLGTITLIYNHHRRQLSEKLIHLQHHHHTAVKVTTHVHLSRELCAEVIV
                     VQARASRVRELADLMRQQKGVLHADLTMSSTGVMLD"
     gene            complement(16970..17269)
                     /locus_tag="P5316_08930"
     CDS             complement(16970..17269)
                     /locus_tag="P5316_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85121.1"
                     /translation="MPRLLTLALAIAVMMVLPAVVSAAPPSRPLPLTSAHFSELQKLQ
                     ADDEHATVADIRASFKSEHEQQLERTRLADDISTGVMLIGYPALIVGLLICAAPL"
     gene            complement(17296..17943)
                     /locus_tag="P5316_08935"
     CDS             complement(17296..17943)
                     /locus_tag="P5316_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85122.1"
                     /translation="MTTPQHQRQPLGDRKSGPRLAAALAGLACLGCMLGCEQPRRTYR
                     LPPDCLPVRYEAATTHATCLVSSVVMASNYLLGDRRLSERGVQVDLKRLGYDETRVGD
                     FKAYLRQLPERLYLVTLTGQLDAKPPTGLRYWLEQRGYPVICVVNRDPAADPGFNHAV
                     LVIGISKNHKGLSADIVHYLDPSSVQQLHSDPIDEFETIWAGGDHAMMIVCQEPT"
     gene            complement(17940..21152)
                     /locus_tag="P5316_08940"
     CDS             complement(17940..21152)
                     /locus_tag="P5316_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85123.1"
                     /translation="MFRPTWAIHACFVQMVVLAVLTVSQPALSQQTPGGSDETITPEQ
                     RKQIEAAKSVAELTSLAKQYLLVRKEAAARLCVEQICAKEPEQLTHPEVQTSSKQWND
                     FWFTCRAEMRARQLLPKDAAGRVELARWLKQGGVYEPARKLVQEALQIEPGLPDAKEL
                     LKELEPPIQLDFRYALNRPVLLTEYMDQSVKVESGRGYLLLLAPVRYRATEVKLSLIP
                     GAIKVTTDVGKMARVLGLLLTEEPPKVDVRAAGGAAVAPIEPTAPVPPVPAKEVPDSF
                     TNTLYERLQIELKSGAPTLTWHNTFVSRPATPAERTTGSPRTAGGARGTTGGTSAREA
                     LAEKRGEIPVNGWLGLLIRFPEEANVVTIELPEAPPESIDLKLLRLTQQQASSGQPST
                     AKPDESLKAVSKFTADPSAPTAQLALAWIGRNVSAQTGPTGQASVAELEAVRALLAAG
                     GHPDRRVRRAGFDGLMEYPSSLPEQALDYLREEADEKTLAGLLEGVSDVLAAGTSRDT
                     TAASVFPSGPAEPGLSKILEAMPASQAPANAFVILGACVNSKRDQARQEALSILLNDG
                     TQQSLQILADRELRKSALKALAGQFSSIKNPDLKAAILRMLLVNPDSATVNSCLAACG
                     ELVVKVSSDEDPLLTALRAEKEKVDPKAKQALLLLLSRSDLSDVANSPKFVEILKSVK
                     EENDKEPAVKSALLTMAASQLNLAYQAPFPRTMQVGTPSPEDQATACEKVLAEGAMDP
                     KVDRASAEKTALMLVASGRINALKDEFVKSRDAERCTGIIQTLAKTKELWKRESLPVF
                     LAAALGNESEEVRKAALSALAILHDNSDKNDRWRVNLAIQQGLDINKIVELTFSPVTR
                     VAEQATTLLKRTIGMTSQEARDFDASGGVPARVTCLTERILGERAKNPVGQYACLVLV
                     DAEITAQPGTTPTGPTVTQTGPVRRTNIPLASGRVAIRRGNGKSLEILADDVEIGRLS
                     GPEDGAGGSASSLQVGSLPINPGELLAEALRSDDARREGLSGKVNLRSVRDGLKQEKC
                     ELKPEAMGGWSAELTISPQGASDSPIRIVGAKIILQPLIP"
     gene            complement(21040..>22089)
                     /locus_tag="P5316_08945"
     CDS             complement(21040..>22089)
                     /locus_tag="P5316_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRR85124.1"
                     /translation="RSIGLQPHAMRGHKSERSGRRRERQAHPRARKPYLLGGLIRCRH
                     CGYGFWGVAIRTLRRGSVRYYADGGFLARGLAVCRSTQIRADELEAWVLGKVRDMIFG
                     NAETMTAAVDRFVKNILAKDGGNGHDIAAVEKELAALNKRIRATAAMLADSNLADLEE
                     LKATLVELKRRREVLEARKTQVAANGEPLDERALRQWATDKFRRMADAAEGSLPLPET
                     KQLLQTFIDRIEIDPERRFGCMYVPADAHAHLAETTRRGHHWALAGPCVMPTTMVQGD
                     SLGGPRALCQAEALLYNENPQTQPPARDKIRYGGSADVQTHLGHPRLFCANGRLGCVD
                     GFATGPVPTNPRRLG"
BASE COUNT         4160 a         6926 c         6546 g         4457 t
ORIGIN      
        1 gttcgagcgg aagtcgatgc acgaccgccc ttccaccgga cgagcggttc catggaaaac
       61 aggacatttc cgccttcatc gtgccaggtc accgccggtc cctgttgatg gtcaaattcg
      121 acggtgattc cggccggcgt gtctgcgtgc agtatcaaca gcagcacaat cgtccaaacg
      181 cgcacaacta tgaccttctc cgtcagacaa ggattgcttt ccgccgacaa gaagtgttcc
      241 tcatcctcgc gcgcgagccc cgatatcgcg acgatagtag gcaccttcga aatgaatctt
      301 ggccacggcc tcgtaacagc gtttacgggc ggcctcgaac gtctctccca gagccgtcac
      361 tccgaggacg cgtccccctg ccgtaacgac ctgaccaccg accatcctgg taccggcgtg
      421 aaagacgacc acatccggga ttgcattcgc ctcggccagt cccgtgatcg gcttgccgcc
      481 ctgatagtcg cccgggtaac cgccggatgc catcaccacg cagaccgctg cccgccggtc
      541 ccaatcgagc gtgatgctgt cgagcctctc gtcgatcacc gcctcgaaga catcgacaag
      601 atcggtctta agcctcatca tcagtggctg ggtttccgga tcgccgaagc gacagttgaa
      661 ctccaggacc ttcggcccgc cggtggtcag catcaggccg gcgtacaaca cacccttgta
      721 ggtgatcccc tctgtgcgaa ggcaatcgat aatcggaacc agaatctccg cctcaatctg
      781 ccggagtgtc ttgtcatcga ccaacgtcgt cgggctgtat gctcccattc cgcccgtgtt
      841 cgggccggtg tcaccctcac cgatcggctt gtgatcctgg gcgttttcca tcacgtagat
      901 gttgtgacca tcgacaaacg ccagaacgct cacttctggg ccggtcagtc tctcctcgac
      961 caccactgtg tcacccgcgt ccccgaactc cctggcaacc atcacacgtt cgagagcaat
     1021 caaggctgcc gccgggtcgt cgcaaacgat cacgcccttt cccttggcca agccggcagc
     1081 cttcacgact acccctgcgt cgcgcgtggc cacatatgcc tttgcgtcgc gatacttggt
     1141 gaaaatcctg gcctcggcgg tcggcaaaga acgatgccgc atcatctcct tggcaaaggc
     1201 tttgtcccct tccaatcggg ccgccgcctt gcacggtccg aacgcccgga tccttgcggt
     1261 cgtcagttca tcgaccaacc ccatgcacaa cggatcctcg ggccctacca ccaccaagtc
     1321 gatcttctgc tcacgggcaa acttcacgat ccctggaaca tcttccgctt tgataggcac
     1381 gttttcggcc acgtccaccg ttccggcatt gccgggagca cagtacaact tgctcacccg
     1441 ctttgactgt gccagtttcc atgccaaggc gtgttctctt ccaccgctac cgaccaacaa
     1501 aactcgcatc agacagactc caatgatggg ctgcggttca tgtccccgtt ttgtgcccga
     1561 cagattaccg gcagaggggt gattggcaag ggcgtcatgc aatccgaccg gcacggccgg
     1621 ggatcgcaat cgtgccgcaa ccacgcccgc gtcagggatt ctgcccacca acatccgctc
     1681 gggcttacgg atctttacga aaacgtacgt tcaggcgaaa atgcgccccg cgaatgggcg
     1741 tttttgcccg cgttgttctc agaagccgga tcgcagacgc ccatccgtca acgcggctgc
     1801 cactgccatc agccttacgg atggacggtg cctgttggga cgaagcatga tgggcaactt
     1861 tcgccagatg cacggggccg gttttgccga tcctattagc cgaccaccag catgatgggc
     1921 aacaggcaag gcatggagtt tccgggaacc caaggaagct agtcgtcgag gctaagtcca
     1981 tatgtttcta acacttgcga ctcttgccac accgacggct ccggccgatc ctgtgttcgc
     2041 gtcctgggga gatgtttttc gcgcctgggg cgggatcgtc cgacagttct ggttggttgc
     2101 gctagcggct ttcctggttg ccctgatcgt tactccgatt gtccggaaaa tggccctgag
     2161 cagaggtatc gtcgaccagc cagatgagtt cctcaaacct cacgggcacc ccatccccta
     2221 tctcggcggt gtagcgatct ttgccggatg ggctactgcc atcctcctag ccttgtggat
     2281 gacgaacaca atggtaacga gctgggtgat ggtcggcgtc gccttggcag gctttgcaac
     2341 gatgttgatc gggttgttcg atgacctgcg agtgatgccg cccctggtca aactggcgtg
     2401 caatatcggc gttgccgtga ttctcatctg cttgggaatc ggcgatgatc tcatcaacgt
     2461 ggtgacgcgg tacagcggca tgcagttcag ccaggaagat cgctggctcg agctcatgta
     2521 ctctgtaccg gtaaccatcg tgatcgtggt cgccgcctgc aacgccacca acctgattga
     2581 cggtctcgac ggtttgtgca gcggcgtcct cggcattatc tccataggct tcgtgatttt
     2641 ggccgttcat ctgcgcgtgg tgtatccgag ttcgccactg ggcgatgacc gcgtcgtcct
     2701 ggccatgggc atgcttggtg cggccctggg ttttctgcct tacaacgtca accctgccaa
     2761 gattttcatg ggcgacgccg gatccatgct gctcggtttc aacgccgcgg tactgcttct
     2821 gatgtttgga gaacagcgca tcgtccgctg gatgatcggg gcgaccacca tatttgggct
     2881 cccggttgcc gacatgctgc tggcaatggc ccggcggtgg cgaaatggca ggccgatcat
     2941 gatcggcgac cggagccact actacgacca gctcagggac cggggcctca gcgtgcgtcg
     3001 ggttgcggcc atcagctatg tactcgcctt ggcgttcgtg attgtcggca catccgtcat
     3061 tttcatccgc acccggtacg cggtcctcgt cttcatgttg accattggcg cggtgctggc
     3121 cgccatctgg agattcaata tggccgggat cgagaaagag acacccgcga cccacgaccg
     3181 gcgcaagcct ttctcccctc cgcccccggc aaaccggaga agcacaacct gatgagccag
     3241 aaccatctcg aacagcgtcg caactggttt cgcttctttc ctgaaggcag cctggagttc
     3301 gtcgtcatca cccgtgtcct ggccctgttg ctgatgctca tgctcatgat cgtcaaggga
     3361 actcaggccc cgttgatcct gctggccctc ctggcaatgc tctggctcga ccacatgctg
     3421 accctctcgt ggaccgtgca actggccacg gatctggcca gctttgcaga aaaacctctt
     3481 ccggaagcgg accaggaagc ggtccgattc ggtccaactc tgctcatcct ggcgctgccg
     3541 atcacgctct tctttgctgc agtcgcgcct tggccggcgg ttttccttcc cccgggaacg
     3601 ccccgtgaga cggccaacgc cgttctcaaa ccgttgcttg gtctggcgtc agtggcaacc
     3661 ttgcttcctg ccttccggcg ggcaaagatg ctgcagatcg gtcggacctt ctggacgttc
     3721 gcctatctct tgccgctgct ccattgggtg gggattcacc gcctcctggc ccccctggac
     3781 acgaggctgt tgaaccagct ccgccggtca gccgcttcgg atgaagccgg ccccggggcc
     3841 ctcgctgccg cagccgacgt cctttggatc atcagcgtcc tgccctggct cgtcatggcg
     3901 gccttgacat tcatcagcct tgaaccggcc gcttcgggcg gaatggcggg ccggctggcc
     3961 tgcgtgtgcg gcactctgct ctttggtctt tactccgtgg tagatcttgc agccatggag
     4021 agggtgcaac gacgattcct gcgactgatt cgcagtcgat gaccaggcga acaatcacat
     4081 tcccttgaag agatccgcga gtttgacgcg taaagctctt gccagccgaa acagtgtcgt
     4141 cattgatggg gcactcagac ccgcttcgat ttgcgatatc tgggcctggc tgatgccgat
     4201 acgttctccc aaagccttca tcgtaaggtc cgtctcgtgc cttgccagtt tgagtcgctg
     4261 cccgagttcc gcgatgaact gttcctcgct gttgatcaga acgccgtcgc tggacagcac
     4321 gaactcaacc gcctccacga gagccgcagg cgatatcggc ttagtcagat aatttctcgc
     4381 gccgagctgc attgcctccg tagccgtccg cacagaggga tgccccgtaa tgatgatgat
     4441 tgacggagac tcgtctcggc ctcggacatc ccgaatcagg tcaatgcccg actgatcggg
     4501 cagcgacaga tcgacgagca ggagatggta cttgcgttga tcgacggccc tgcgggcctc
     4561 ctgaaacgag ccgaccgcat ccacctgaaa tcctgctctt tcgagcgtcc gagcaagaac
     4621 gcgctgaacc tcaatctcgt cgtccaccaa cagaatctga gcaccatgac gtggcatgcg
     4681 gttgatctcc caaacggcgg tggtccgttc gatccccttc cgaccacctc ggccgtacat
     4741 tctaccggag cagcacaccc cctgaaaaga cgccgatgga agcgtttcgc gaaatcggcc
     4801 gagcgggatt cagtttggct tgcccctacc ggatgccgat aatccaggga aacagtctgg
     4861 gggagatatt cttcacccat tcggagtcga cgcaatgaga ctcactgtca tcggtacagg
     4921 gtatgtcggt ctggtcaccg gtgcctgcct ggccgacact ggaaacgaag ttgtcggagt
     4981 cgacaaggat gccgagaaga tcaatacgct cactcggggc gagtgtccca tctacgaacc
     5041 cggcttgtcc gaattgcttc aagccaacac gaaggccggc cggctccggt tcaccgccga
     5101 catccccaag ggcatcgaac acggagacgt ggtcttcatc gccgtcggca ccccgcctcg
     5161 cgatgacggt tcctccgaca tgaccgcggt ccgggaggtt gccgacaaca tcgccgatca
     5221 cgccgaccgg ccgaaaatcg tcgtgctcaa gagcaccgtt cccatcggaa caggcgacga
     5281 gatcgaagaa cgaatcaacc gccgggcaaa acaccgcgtg aacgtcgtca acaaccccga
     5341 gtttctcaag gaaggaaccg cggtggagga ctttctcagg cccgatcgcg tggtgatcgg
     5401 tgccgaggac ccgcaagccg cctcggtcat cagggagctg cacgaaccct tcgttcgcaa
     5461 tcagcgaccc atctacatca tgcaccgcaa ggccgcggag atgagcaagt acgccgccaa
     5521 ctcctacctg gccgcccgca tcagtttcat caaccagatc gccaatgtct gtgatgccct
     5581 gggcatcgac gtgaacgaag tccgactcgg aatgggctcc gatcgacgca tcggtttcca
     5641 gtttctctat ccgggcgcgg gctatggcgg cagttgcttc cccaaggatg ttcagtctct
     5701 cgtgcacacc gcccgaaaag ccggcgtgca ggccgatctg ctcgacagcg ttcaccaggt
     5761 caacgagcgg cagcgggaga tcctcttccg caagatcgtc gctcgattcg gcggatcatt
     5821 gaacaattac tgcttcgcga tatggggcgt gtccttcaaa cccaagaccg acgatattcg
     5881 cgaagccccg gcggttacga ccatcaacaa gctgctcgaa gcgggggccg ccgtccgagc
     5941 ccacgatccg attgctctgc ccaacctccg caagctgctc ggcgaccgga tcacgtacca
     6001 cgaatccggc tacgaagccc ttgatggcgc cgatgctctg gtcatcgtca ccgagtggaa
     6061 cgagttccgc tcgcccactt tcgaggagat ccgccggcgc ctgcgccaac ccatcatttt
     6121 cgacgggcgc aacctctatt cactcgacgc gatgcgaaac cgcgatttcg agtattactc
     6181 gatcgggcgc ccggcggtga ttccgactcg acgctgaagc ccgcgccggt ccgtcgcatt
     6241 tgagtcgtac gtacagggga cacgattcca cgccgttcca ggccacaaca agacgaacgg
     6301 ccggtgggat gggcagcacc ccaccggcct tggtctctta tcctgcagcc caatctgacg
     6361 ccggcctacc gccgtgaagg gaatgtgtcg gtcatcaccg cctctcaatc ggggaccacc
     6421 ggcggcggca gcgaatcatc acatgcctgg tccgcagcca caccaggccc ggaagcgcaa
     6481 ttctcgaacg ctaacaggtc cgtctggtca acgtccaggt cattgttggt gtcgaagcag
     6541 aaacaggccc ccgcgtcgaa cagaaaagcg ggatccccat cgccggtaaa gcacatttga
     6601 agggcggcga aatcagcctg atccacgtct ccgtcgccat cacggtcaaa acgattatca
     6661 ccgcagggcg gcggaacacg ggtaagcgtc agcgacagat tatcccaggc gaccgcctga
     6721 ggaccgggat gcttgtcgtg ctgcagcaga cggacctcga tattacccgt ggtcgtggag
     6781 atcgtttgct gcgtgacgac gtgttgccaa tgcccgttga gactccaatc aaccgtgtta
     6841 ttgagccacc ccggccagta gtcaacgctg aagccgtttc cgcgatcacc attatcaaaa
     6901 tcccatagcg gttggttgta ctggtcgtcc gtcagaacat acatccggtt gccaacgccc
     6961 catggatcac ccggttcctt gtagacatag cagtcgtatt cgaacgttgc gacgtaggtt
     7021 tcccccgcag gaacggcggc cgggaactgc tgctgcatcc acgcggcacc ggtgaagtcc
     7081 atttctgcca tgtttgacga gaagccggcc gactgtgaac ccggattgtc cacgttgtcg
     7141 tagagcagcg ggtcggtggc ggagaacagg attggcgtat cgttgggctt cggtccgtac
     7201 agtttcgtcg tccagccggc caggccgtcc tcgaaatcct ccggcttgtt caccaccacg
     7261 gccgtgcctt gggcgggcaa agcgtcggcg gcacctacgg cagcccaaac cgtcacctcg
     7321 ttctcactga cgctgctcac cttcatgcgg cgtatcccgc cggatcctgc cggtcctgcc
     7381 catgaaacgt cgaccatgtc attcggctgc aagccgtggc ccgcgccgag cgtaaccacg
     7441 ccgttgtcgg catcggttcg cgtggtcagc gttccggccg tgcccgccgg gtaagtgaac
     7501 acaaccgatc cgcccggccg gcgaagagtc agtgtgagat tgtcccaggc cacggtgttg
     7561 tagtgcgcgt acttctcgtg aaacaaaagc cggagttcga cgttgcccgt ggttgtctca
     7621 atatcctgac cacgattgat ggcgccgatt tcccagatgc cttccccctt attggaccac
     7681 ttactccagc gaacaccgtc gatgcccatg tcgccatcgt aattcatgac gggattgtcg
     7741 tatttgctgt cggtcagcac gtaaacgcga ttcccgaccc cccatgggtc ctcgtcgcct
     7801 ggtccgttcg gcggatcgtc gccgtcaaag tagacatatc gatctacgac gatcacgacg
     7861 tcatagatgc ccggctcaag tagaccgggc cactggatct cgatccattg gggctgctgg
     7921 ccgtcccaag gagccagatt cgacgaaaaa ccgactgact gggagccagg gtagccgatg
     7981 tttgcaccgt agatgccggc tcccgtggaa tcgaagacta gcggcgtatt gttgttcacc
     8041 ccccccgtcg ggtcatacgc cagcactgtc caagcgtcca atcccgactc gaagtcctct
     8101 gtgaaaatcg gatactgcgt ctgacctgaa gcgggaatcg cgacagccac gaccaggcag
     8161 aaaccccaaa accatctgca catgacaggc cctccaagaa acgagaatgc aagaacacac
     8221 accttcccaa cggagcaacc aagtgacctt caggcaattg gcccaagcta cgaatcctgc
     8281 gtcgccctcc tgccataacc acgattaccg cgcagggtct tgggtaagag ttgcactaaa
     8341 cccgcgctct ccatcgcctc gtatcacgat gcatcatacc ttgcggacgt tcttgtggtc
     8401 aagaagaaaa cgggattccg tccggacatg cagcggcttt cttggccggc tttttggatt
     8461 catggaccat tcgtgggaca gcatcggacc cgcatcagga gaagtcacgc gttttaccag
     8521 ttttgttaat cagaattcag tgacgctggc ctgaccggaa tcaacggttt tcttgccgcg
     8581 caaagcgagc gacccgggcc gacaaggccc cgattggtca ggcaggcgaa ggccgttctt
     8641 gattctctca ttctgttact cgaccggcct cgatgccgcc aaacgcagca catcccaatc
     8701 caggcgggcg gcaaccaacg tggccttggc ctccgctatc cgatcggctg gtgagcgtga
     8761 ggaccagtaa gaggccgaga gtagctcatt cacgcatggg gtgggcgtta cctgctgcgt
     8821 tttcgtgtct atcaggaaca ttgccccgcc ggcggacggc aagacagccg caaaaggctg
     8881 aaagtcatcc gggtacggta gctgcttgac cacgatcctg accgggggag ccgggttctg
     8941 gcctgggagt ggcatcggtt cgcaacggtc ctcaccgggc acccaccgat agagtccccg
     9001 ttcggtgccg aggtagagaa ctccggccct gctggaccga aagatgctgt agatctgaac
     9061 ctgccggcca agctgtcggg ccagcattgt tgacagattg aacatccggt gaggtttcgc
     9121 ctcgcagtac aaccaggtgc cgtcgcccaa catgaccggc tcgatgggag cattgttgtt
     9181 gtggtcgggg aactcctccg gcagaacgat ggagcgcgct tggggcgggt gcgatcgcag
     9241 ttcggccgcg agctcgtcgg cccacggggc atacttatgg atcgcccagc cacgctggct
     9301 gaaggggatg tgcatcagca acgaccaacc tgccgcgtcg ggctggagct ggtagacctg
     9361 ctcgcggctg acggccaaca ccgtacggcc gctgtccgac acgatgagac cgtcggccaa
     9421 cctgtaaggc caccaacccg gctcgggcaa cgggatctcg gtgacccggc catcaggcga
     9481 tgcctttccg atgcgaatgt cccgcgtaat caggtagcga gtgccgtcat ccgtcttgta
     9541 ggcgtacagc acgcgccgcc acttctcgtc ctggccggtg aggtccttgg cgtgttcaga
     9601 taggaaccga acagcctccg tctcaggcaa gatgtcccac ggcttgttcc agggcttgtt
     9661 ctcctggtca tatgcccgta tcttctcgta ccaatcaagc gacgccgcgt aattgccttt
     9721 gagcgcctcc aatctcgccc ggtgaaagct cggggtcaac cggttgcgct gcgctgcttc
     9781 gcagaattct atggccttct cgagttttgc gacattcggg tccacctgaa aagcgagggc
     9841 gctcaagagg tagtcgaggt tcatcgccgg cgcgtacttg agctcttctt cagcgtgccg
     9901 ggcccaaaga tcggcggtct tcggccacca ggccgagtag aggcgggcat cctgagcaaa
     9961 gcccaagtcc gccagacctc tggccagcgc acggcgaaag tcatccacct gcccctgccg
    10021 caggtagact tgatagaggc gatctttcgc atgtatgtac tcccagtagg cctgctcgcc
    10081 ctctgcgtct gatccttcgg gaatctgtgg caagacgtcc tcgcacaatt tcagccggcc
    10141 gatcctgacc gccgcaggaa agagccaccc aaaccgcacg cggccctgcg gaaaggccgc
    10201 caggcgatcc agcacgaccg acaattcggc atccgcgccc agcgcgtcca ggcaggttgc
    10261 cagcttgtgg agggcgtgcg cctggtcttc cggtcggcgc ttctcccccg cgcgggccgg
    10321 gttgagcagc tcgaccagag cctcggcgta gcgccgctct ttgaacacca gccgatacgt
    10381 gagctggtca tgcgcccact ccaccatgtc gccccccctg atgatcctcc ggcaaatctc
    10441 gtcgtgcagg tggaggtctt cgggattgag ggacgttgcg aatccgatga tggcggcaag
    10501 gtgatttatg tacgggggtt tcttgaggat catgtccaga tacgccggcc gttcggcagg
    10561 cggcagggcg taatagcggc atcgggtaat caaacccagc gttccctgga cggcttccac
    10621 atcgacgccg agctcgtttc ccgcctcaat ggccttctcg atggccgcga ccgccgcggc
    10681 atagttgccc acgtcgtgtt cgcgggccgc ctcggccaga tgatcgcccc aacccgactg
    10741 atacatacac cattgtatac cgtattttcc ttctctaaac ttcctatcgt aaactcgctt
    10801 atcccgtcgc agacgcaccg tccgcgcccg attccggaag cgggtctcct cggctttgga
    10861 ccaggcctca acatcgctga tcgcttcacc atcgaccgcc agaagaacat cccccggtcg
    10921 aacgccccca taccatgcct gcccgcgcgg aaataccagc agcacgagcg ccccgcgacg
    10981 cgacagccga atgtccttca ggatgccggt gatggctatc cgggcctcgg ccggctggtc
    11041 gggcagggcc ttctccaggg gttcctccgc agcgtcgccg agctccgtca atcgctcagc
    11101 agcagcctta cgtcgcgcgg gttgcggatg gttgagatcc cgaatgaggc gaacgatttc
    11161 cccgtcgacc accgcggcgg gagcagatgc cggagccgac cgggtccata gaacagcggt
    11221 taagagcaga gagacagccg tcgccatggt ggattccctc cataactcca cgctacagtc
    11281 tatcaccttc gattacagtc atcaaaccac ccggtcgaac cgcctttttg cgggccgcaa
    11341 ctcaagcgca gtgcgatgcg gaaagaggcg ggcaaaccgc atgaatccgt tggctgaacc
    11401 catgccgatc tgcgtcgtct gcgtctacag gacaagcccc cgacacaaag caggcctcgc
    11461 cgggcctact cggctcgggg gcaagttgag tgagatggat tcccaagatg gttgacagcc
    11521 cgggagagcg ctttcctcca aaaaccgatc tgctccctta ccgatgccgg ccccgcattg
    11581 ccggggctgc gataacgcgc cacgacgttg gccgctccaa ggtattggta cacgtccttg
    11641 ccgatccgat cgcacacttt tgtgaattcg tcgagcgtca gaccgcccag ttcgcggttg
    11701 tctttctcgg cgtatgccac cagcgtgccc acgtgatgat gagcctgacg aaagggaatg
    11761 cctttggcga caaggtactc ggccaggatc gtcgcgtcca tgaagcccct gtccgtcgcg
    11821 gccaacagcc ggtcggtgtt gaaggtcgtg ttacgaatca ccgccgccgt gatctcgacc
    11881 gacgccttca acgtatcgtg agcgctgaac aggtggatct tgtcttcctg gaggtcgcgg
    11941 ttgtaggcca tcggcaaccc cttggtcagt gcgagcatac ccatcagggc ggcgtagacc
    12001 cgaccggtct tgccgcgaac cagctccatg acatccgggt tcttcttctg cggcatcatc
    12061 gacgaacccg tgcagaacgc gtccgcgatg gtcacgaacc cgaattcctg cgacatgtag
    12121 aggaccagat cctcggcgag acgcgacagg tggcacgcac acagcgccgc cgccgccaga
    12181 aactcgacga tgaaatcgcg gtcggcggtc gcgtcgacgc tgttacgggt tatggaggaa
    12241 aagcccagtc gcttggcaac cgcttcccgg tcaatcggca gggtcgagcc ggcgagggcc
    12301 cccgatccca gcggacagac gtccaccctc ctgcgggcgt cggaaaaccg gtcccgatct
    12361 cgctggaatt gctcgacata ggccaagagg taggcgccgg cactgatcgg ctgggcccgc
    12421 tgcatgtgcg tataggcggg catcaccgca tcggcgtatc tctcggccga gtcaaccagc
    12481 gccgcctgga ggtccgccag gagggggagc acatccagat cgatcgcatc ccgggtgtac
    12541 agtcgcaggt ccagagccac ctggtcattg cgcgaccttg cggtgtgcaa ccgcttggcc
    12601 ggctcgccga ctttggcgat caaggccccc tccacggcca tatgaatgtc ctcgcaggcc
    12661 ggatcccact tgaatttgcc tgcctcaata tccgatgcaa tctccatgag gccgcgtttg
    12721 atcgcccggc agtccgactt gccgatcagc cctacctcgg cgagcatctc ggcgtgggcg
    12781 atgctgccgg caatatcgta tttccagagg cgtcgatcaa aggagaggct ctcgacgtag
    12841 ttttcggtca gtacgtcggc cgcctgcgag aagcgggcct gccagctctt ttgcgctttc
    12901 atagatgtct ctccacgggg ttgcgcctcc ttaccggtgc cctcgtcggc gcctgggtct
    12961 cgttttctcc ttgagtgggc ggccggcgac gacggtcacg gcggttcggt ggtcggatgg
    13021 tatgcaaacg gcggcagtgg gtcaatcgca ggtgcggcca agaggcggaa acgagcatat
    13081 aatcaggaga tgcgaatgaa tcggttatgg gcatggtgcc tcgccgcggc gtggcttgcg
    13141 gttgcagccg gcgaagccgc gaccacgcgc ggaccggttt tggagcggga tccctcgcag
    13201 gaggccttgc tggccaagca ggccggtcca aacttcaagg tcaaacgcac gtcgcatttc
    13261 ctgatagcct atgacacggc cgacgaggtt gcccgcgaac tggatatccg cctcgagcag
    13321 acctatcaca tggtcttcct tttctgcgag atgaacggcg ttcaggcccg ttggcctgat
    13381 cgccgcctgg aagtcatctt cttcgacaag cggcccacct acgaacggca tgttgcccgc
    13441 ctgggcgtca acgccggggg caccttcggg atctaccacc agcagactaa tcggtcctat
    13501 tttttcaatg ccaccaacga cgagcaggtt ctcaggctca aggagcaaat cgccgcgagc
    13561 aacgccaaca tcaacaatct tcagcagtcg ttgcggaaca tgcccgccaa cggcggcatc
    13621 atcgaggttc ggttttcgga cggcgaacgc atcgtcggca gcaaggccga ggtggagaaa
    13681 cgcgtgaaca agaagctgaa cgaggctcgt gctgagctca agaaagtgga ggcccgccaa
    13741 cagagatact gcgaccagat caatcagacg gtgatccagc atgagaccgc tcatcagacg
    13801 ctgttcaacg cgggtctgca cgttcggggg gccggcaatc ccaagtggct gattgaaggg
    13861 ctggcgtgtt tgttcgagac gccgccaggc ccgcccggca gcggcttctc ggcgctgaac
    13921 gcagcacgtc tggaggattt cagagtcggc gtcggcggtc aaaccaaacg acggctgacc
    13981 agccgcgatt acgagacggc cattcagacc ggccgtatgg tctcgcccaa ggagcttatc
    14041 ctgaatcccg agcttctcaa cgctcgcggc gatgcaggcc tgcacaacta cgccgtcgca
    14101 tggggcctgg cacactacct ccagcgcaat cagcgggaac agttggccgc ttacctgttg
    14161 gacgtgagct cgcgtctgcc cggggacgac cccaccccgc agcaggagtt ggagttgttc
    14221 gaggagcatt tcgggccgat cgatgagatc tttctgcgga agttctcggg ctacatcctg
    14281 gggcttacgg ccagggccca gcttgactcg tagcgccggc tcacaacggg gtcggattcc
    14341 tattcggtat cctgtccggc agtcggaaca ggtccggaag gtactcttgc tccgacctct
    14401 tcgcgcaaga ttccatttca gaaccgcctg gatataatgg acattcggtg gagcgcggcg
    14461 accggtgcgc gggtcgcgcc gcgggactct ccaccgtcag gacggaatcc atgccggcaa
    14521 ctgcgagaca caaggcgtta cggctgacgc tggcggctgt actgggagga ggaacgctgc
    14581 tatcgccggg ctgcggcgac attcttcggc ggtccctgcg ggatggagcc atcgccctga
    14641 ttgcaggcac cgccaccggt atcatcgacc cctacgacct tagcaacctg gtaaccaaca
    14701 tgttcaccgg cgggttcacc ggcggttgga acggaatcgg cagcaactgg aactgggact
    14761 ggggctggaa cacctcgccc ttcacctggc cgggaacgtg aaagaacggt cacaattggg
    14821 tggcctggag atccgtgcgc acgggggacc gaggggggag atgctgcgcg tgatctctca
    14881 ttcgaggcag cttgccgggt ggggcagcag gcgtattgcg gcctgctgaa ggaacagggc
    14941 cggcacgcgg cttcttttga tgatgaatcc gagaaaacaa cggccggtgc gggtggaccg
    15001 gccgttggga aaacctctcg gttatgcggc gttacagaag ctcggcgatc tggacggcgt
    15061 tcagagcggc gcctttgcgg agttggtcgc cgctgatgaa catgtcgatg ccgcaaccgt
    15121 cgggttggct gatatcctgc cggatgcggc cggtgaacac gtcgtcctgg tccgacgatt
    15181 caatcggcat cgggaagtaa ttcctgacgc ggtcatcgac cagctttacg cccggagccc
    15241 tggccaggat ctcacgaacc cggtcctcgc tcatcggctt ctcaaaggtg atgttgatgg
    15301 cctcgctgtg agcccggata atcggcacgc gtacacaggt ggccgtaatc tgaatctcgt
    15361 cgtcgtggaa catcttgcgg gtttctttaa ccatcttcat ctcttcttca ttgtagccgt
    15421 tctcaccgac tttgctgttg tggctgaaga gattgaacgc gatctgatgg ggcagaacct
    15481 tcttggtcac cggcttgccg gcgagaaagt ccctggcctg ttgctcgagt tcgagcatcg
    15541 cctggtatcc ggccccgctg gcggcctggt aggtgctgat gaccaaccgt ttgatccggt
    15601 tggccttata cagggggaag accggtacaa ccatgatgat cgtcgaacag ttggggttgg
    15661 cgataatacc cttgtgtttc ctgacgtctt caggattgac ctcgggggtc accagcggca
    15721 cgtccgggtc catccgaaat gccgaggaat tgtccaccac cacgcagccg gctttgacgg
    15781 cgatcggggc gaattggcga ctgatcgagg ccccggccga gaacaaggcc aggtcaaccc
    15841 ctttgaagct ggcgtcggtc agctcctgca ccgtgtggtc cgcacccctg aacttgatcg
    15901 tcttaccggc cgaccgcgcc gatgccagga aggtgatctt gtcgcacgga aagctccgct
    15961 gttcaaggac gcgaatgaat tcctgcccca cggcgccggt ggcgccaacc actgccacgt
    16021 gttttgccat cgatgacttc tcctgcgcaa agtgctcctg tttaatcttt cagctactct
    16081 tcttctctgg caggggagac cctggagaat gcaccaggcc aactacggcc ggaactccgg
    16141 catgatagag gattgctcgc caagatgcaa agtcgggccg agccgaaaca gaaggtccgg
    16201 ttcggcaaag gtttccggaa gcgatgctga aacgtcggac gtcccgataa gggcccgtcg
    16261 cctgtcgcgc cacgggagtc cttgacagac tgaccgggcc ttcctagctt tcccattgag
    16321 cgttcacagt cgtctttcgg gaggcgtcgg caatgtccga actgatcaga ttgagcctgt
    16381 cgatcgagaa gccgctctat gatcgtctcg agaagttggt caagcagagc ggctatacca
    16441 accgttcgga gtacgtccgg gacatgatcc gagagaggct tgtcgagacc gaatgggata
    16501 aagatcgcga agtcctcggt acgatcaccc tgatctacaa tcatcaccgc cgacagcttt
    16561 ccgagaagct gattcacctc cagcaccacc accacacggc ggtcaaggtg accacccatg
    16621 ttcacctgtc acgtgagctt tgcgccgagg tgatcgtagt gcaggcaaga gccagccgtg
    16681 tccgtgagct ggccgacctg atgcgtcagc aaaaaggtgt cctgcatgcg gatctgacca
    16741 tgagcagcac cggcgtgatg ttagattgac cgcccgcagc gcccgcccat cgtcgacggc
    16801 gagggtttca ggcagccgtt tgcgtgattc tcaaagtgtg ggcggcttca acatttcagg
    16861 gacaaatgga ggtctggccc gagaggttca ccctgtcatg ccaaggcaag ctcggccgac
    16921 tgaaagtcga tccgatgatc ggccagaaag cggaaggtgt aaatccgcgc tacagcggag
    16981 ccgcgcagat caacaggccg acaatgagcg ccgggtaccc gatgagcatt acgcccgtgg
    17041 agatgtcatc cgccagacgc gttctttcga gttgttgctc gtgctcggat ttgaaagatg
    17101 cacggatgtc cgccacggtt gcatgttcgt catcggcctg cagtttctgg agttcgctga
    17161 aatgcgcgga cgtcaggggc aatgggcggc ttggcggagc ggctgagacg acggcaggca
    17221 ggaccatcat gacggctatt gccagggcga gcgtcagtaa gcgcggcata ccctatctcc
    17281 cttcaagata agaccttatg ttggctcttg gcaaacgatc atcatggcgt gatcgccgcc
    17341 ggcccagatc gtctcgaact cgtcgatggg gtcagagtgt agctgctgca ccgacgaggg
    17401 atccagataa tggactatat cggccgaaag gcccttgtgg ttcttggaaa tcccgatcac
    17461 aagaacggca tggttgaaac ccggatcggc cgccggatcg cggttgacca cacagatgac
    17521 cgggtagccc cgttgctcca gccagtaccg gaggccggtt ggcggtttgg cgtccagttg
    17581 gcccgtcaga gtgacgagat acagccgctc cggaagttgt cgcaggtagg ccttgaaatc
    17641 cccgacgcgg gtctcatcgt agccaaggcg cttgagatcc acttggaccc cccgttcgct
    17701 cagtctgcgg tcgccgagca ggtagttgct cgccatcacc acgctgctga ccaggcatgt
    17761 cgcgtgtgtc gtcgcggctt cgtagcgaac aggcagacag tccgggggca ggcgataggt
    17821 tcgcctcggc tgctcgcaac cgagcatgca gcccaggcag gctaacccgg caagagcagc
    17881 ggccaatcgt gggccgctct ttctgtcgcc gagaggttgt cgctgatgct gcggagttgt
    17941 catggaatca acggctgcaa gatgatcttg gctcccacga ttcggatggg gctgtccgac
    18001 gccccctgcg ggctgatggt cagttcagcc gaccatccgc ccatcgcctc gggtttgagc
    18061 tcgcatttct cctgcttcaa accgtccctg acggatcgca ggttgacctt gccggaaagt
    18121 ccttcccttc gagcgtcatc ggacctcagg gcctcggcga ggagctcacc cggattgatc
    18181 ggcaggctgc ccacctggag cgaagaggcc gaccctccgg ccccgtcttc cggcccgctt
    18241 aatcggccaa tctcgacgtc atccgcgagg atttccagcg atttgccgtt gcctcgcctg
    18301 atggcgactc ggccggaggc cagagggatg ttggttcggc ggaccggacc ggtctgtgtc
    18361 actgttgggc ctgtcggtgt tgtgccgggt tgggcggtaa tctcggcatc gaccaaaacg
    18421 aggcaagcat attgacccac gggattcttg gctcgttcgc ccagaatgcg ctcggtcaga
    18481 caggttacgc gggccgggac gccgccgctg gcgtcaaaat cccgagcctc ttgggatgtc
    18541 atgccaatgg tgcgtttgag caatgtggta gcctgctcgg cgacccgggt cacggggctg
    18601 aatgtcaact ccacgatctt gttgatatcc agaccttgtt gaatggcgag gttgactcgc
    18661 caccggtcgt tcttgtcgga gttatcgtga agtatggcaa gcgcggagag cgccgccttg
    18721 cggacctctt cgctttcgtt gccaagcgcc gcggcaagaa agaccggcaa agactcgcgt
    18781 ttccagagtt ccttggtctt ggcgagcgtc tgaatgatgc ccgtacaccg ctcggcatct
    18841 ctggacttga cgaactcatc tttgagcgcg ttgatacgtc cggaggcaac cagcatcagg
    18901 gcggttttct cagcagatgc ccggtcgacc ttcgggtcca tggctccctc ggccagaacc
    18961 ttctcgcagg cagtcgcctg gtcttccggc gagggtgtgc cgacctgcat tgtccgcggg
    19021 aacggtgcct ggtaagccag gttgagttgg gacgccgcca tcgtcagcag ggccgacttc
    19081 acggccggtt ccttatcgtt ttcttccttc acggatttaa ggatttcaac aaatttcggt
    19141 gagttggcga cgtcgctaag atctgaacgg ctgagcaaaa gcagcaacgc ctgcttggct
    19201 ttcggatcga ccttttcctt ctccgcccgc agggcggtca gaagcggatc ttcgtctgag
    19261 gacaccttaa cgacaagctc gccgcacgcc gccagacaac tgttgaccgt cgcgctgtcg
    19321 gggttgacca acagcatgcg caggatggca gctttcaggt cggggttctt aatcgagctg
    19381 aactggccgg caagtgcctt cagggcgctc tttcgaagtt ctcggtctgc caggatctgg
    19441 aggctctgct gggttccgtc gttcagcagg atgctcagtg cctcctggcg ggcctgatcc
    19501 cgtttcgagt tgacgcacgc acccaggatc acgaaggcat tggccggtgc ctgcgacgca
    19561 ggcatggctt caaggatctt gctcaatccc ggttcggctg ggccactggg gaaaacgctc
    19621 gctgccgtcg tgtcccggct tgtgcccgcg gctagaacat cgctcacccc ctccagcagg
    19681 ccggcgagcg ttttctcatc tgcctcctcc cgcagataat cgagggcttg ctcgggcagc
    19741 gaactggggt attccatgag accatcaaag cctgctctcc tgacccgccg gtccgggtgc
    19801 ccgccggccg ccaagagggc ccgaaccgct tcgagttcgg cgacggaggc ctgccccgtt
    19861 ggtcccgttt gagccgagac gtttcgtccg atccacgcca acgcgagttg ggccgtcggg
    19921 gccgacgggt cggcggtaaa cttgctgaca gctttgaggc tttcatccgg cttcgccgtt
    19981 gatggctggc cggatgacgc ctgctgttgc gtgagtctga gcagcttgag gtcgatcgac
    20041 tcgggaggag cctccggcaa ctcgatcgtc accacatttg cctcttcagg aaacctgatc
    20101 agcaagccga gccagccgtt gacggggatc tcgcctcgtt tctccgccag tgcctcacgg
    20161 gcactcgtac cgccggttgt gccgcgggcg ccgccggcgg ttcgcgggct gccggtggta
    20221 cgctcggctg gagtcgctgg gcgcgaaacg aaggtgttgt gccatgtcag cgtcggagcg
    20281 ccgctcttga gttcgatctg gagccgttcg tacagggtgt tggtgaaact atccggcact
    20341 tcctttgcag gaacaggggg aaccggagct gttggctcga ttggggccac agcagccccg
    20401 ccggcggctc gcacgtcaac cttgggaggc tcttccgtga gcagcagacc gagcacgcgg
    20461 gccatcttgc ccacgtccgt tgtcaccttg atggctcccg ggatgaggga gagtttcacc
    20521 tccgtggcgc gatatcgcac gggcgccagg agcaataagt aaccccggcc gctctcgacc
    20581 tttacgctct gatccatata ctcggtcaac aggacgggcc tgttcaaggc atagcggaaa
    20641 tccaactgga tgggaggctc cagttctttg aggagttcct tagcatccgg aagaccgggc
    20701 tcgatctgga gtgcctcctg tacgagtttg cgggccggct cgtagacgcc cccttgctta
    20761 agccagcggg cgagctccac acgaccggcg gcatccttcg gcaagagttg cctggccctc
    20821 atttccgccc gacaggtgaa ccagaaatcg ttccattgtt tcgagctggt ctgcacctcg
    20881 ggatgggtca gttgctccgg ttcctttgcg caaatctgct cgacgcacaa ccgggcagcg
    20941 gcttccttgc gtaccagcag gtactgcttg gcaaggctgg tcagttcggc cacggatttg
    21001 gcggcctcga tttgcttgcg ctgctctggg gtaatggttt catccgagcc gccgggggtt
    21061 tgttgggaca gggccggttg cgaaaccgtc aacacagcca agacgaccat ttgcacaaaa
    21121 caggcgtgga tggcccaagt gggtctgaac atctgccgaa cctccgtagc gaatcttgtc
    21181 cctcgcaggc ggttgggtct gggggttttc attgtacagc aaggcttcgg cttggcaaag
    21241 ggcccgaggc ccgcccaaag agtcgccttg gaccatcgtg gttggcatga cgcacggacc
    21301 ggccagcgcc cagtggtgtc ctcgtctggt agtttcggcc aagtgggcat gggcgtcggc
    21361 ggggacgtac atgcatccga agcggcgctc ggggtcgatc tcgatgcggt cgatgaaggt
    21421 ctggagcagc tgcttggtct ccggcagcgg cagcgaccct tccgccgcgt cggccatccg
    21481 gcggaacttg tccgtcgccc actggcgcaa ggcccgctca tccagcggct cgccgttggc
    21541 ggccacctga gtcttccgtg cctccagaac ctcgcgacgg cgcttcagtt ccaccagcgt
    21601 cgccttcagt tcttccaggt cggccaagtt ggagtcggcc agcatggcag cggtcgcccg
    21661 gatacgcttg ttcagggcgg ccagttcctt ctccacggcg gcgatgtcat gcccgttgcc
    21721 gccgtccttg gccaagatgt tcttgacgaa cctgtccacg gccgccgtca tcgtctcggc
    21781 gttaccaaag atcatgtccc gcaccttgcc cagcacccac gcctccaact cgtcggcccg
    21841 aatctgcgtg ctccggcaga cggctaggcc cctggccagg aagccaccgt cggcatagta
    21901 ccgcactgac ccgcgacgca gggtccgaat ggcgacgccc cagaagccgt atccgcagtg
    21961 gcggcaacgg atcaggccgc ccaggagata gggcttgcgg gcgcgggggt gggcctggcg
    22021 ctcccgccgc ctaccggacc tttccgactt atgaccgcgc attgcatgcg gttgaagccc
    22081 aatcgacct
//
LOCUS       DAOTPW010000078        21976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2446_length_21976_cov_5.473245, whole genome shotgun sequence.
ACCESSION   DAOTPW010000078 DAOTPW010000000
VERSION     DAOTPW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21976
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2446_length_21976_cov_5.473245"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1654)
                     /gene="dacB"
                     /locus_tag="P5316_08950"
     CDS             complement(170..1654)
                     /gene="dacB"
                     /locus_tag="P5316_08950"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRR85125.1"
                     /translation="MRFRSIHVRSAIIGLLSVCVWGRVAWAGPYEALGDEIKALAAAP
                     SLAKVKVGIRIVALGIEQPQLVYAQNADELFKPASNQKILTTAAALTMLPPDFQYRTL
                     LGMLGRDLVIIGAGDPSIGDPKLAKAQRQPVTAVFERWADRMKAEGIHAVEGDLIFDD
                     SIFDTEFVPASWRQQHNLEDWFTAPVGGLNFNDNCVDVILTPAEKGKPAIVELVPDWV
                     KVDNKSVTGSKGQPVIRRASVDPLTISVSKTVSRPTGDGNPTSIPVGDPGDFFARTVH
                     SVLSAKGIKIDGKVRRQRVRQADGSLPAEFKIVAAYESKPIDFLWRMNKSSLNVFAEA
                     LLKTLGAYSTGGKAVGTRAAGAAVIREFLAKLGVKTDGFVIDDGSGLSHNSRISPTSL
                     CETLVYVDRQMPIRKQWWAGFATPGDPDGTLRNRMPALKGTVYAKTGYIAGVSALSGY
                     VVAADRKRYFAFSILCNDTNKSKDGSTAARRLEEEICKRLAAFK"
     gene            1874..2650
                     /locus_tag="P5316_08955"
     CDS             1874..2650
                     /locus_tag="P5316_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85126.1"
                     /translation="MSRFQPHGARILCVAGSEGEFPEAVRQWLAQVTLRTEYCSDVYD
                     ALAVLATGTRPILVIVGIDSVDWSEMDFFSHVAALSRGTRIYVAAHDASDGRVFAAGE
                     RGARLFDPASANADIEAVFTQDQASGPGGLLAGTLRPSQVNRQPVNVPPATAEQNQPP
                     ASETFKTKTEEPPIRLVTAAEPDENSDVELSVPWAPHPSRPRRTPPKGPADAPQPSPR
                     QTDGGIETDKPLPRVELTPDEIAALLGKPGSSPRLREQGS"
     gene            2647..3963
                     /locus_tag="P5316_08960"
     CDS             2647..3963
                     /locus_tag="P5316_08960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRR85127.1"
                     /translation="MTPDPLIPTREQLLIVHDPADLAAFVRGRYPQFDVATAPGYMDG
                     LVALARQSPRGVLVGVEPTVRKLSNAIAALRKAAGPSSRIVLCCMPSGEPVARRVVAA
                     GADDYLIYPPEGAELDAALALPTPGGDARLATPEPPVPTWYELHSLTDILAGLADGRT
                     AMLERLCRLLADAMRTPFVRITLERESRHVGDPKTEPALFETIQSGGQVLGKIEVGPR
                     HRGPFSAAEVEKLRHHGRLIAHLLKAAEQQQQWHRLAMIDEVTQLPNRRYFRQALETL
                     IRRAATERFSVTVVIFDLDGFKHFNDTYGHNAGDQVLRELAQLIRRHCRRHDIVARYA
                     GDEFVVAFWDAEEPRVAGSKHPTDALAVLRRFQKTLESQEFANLGLDSKGRITISGGL
                     ATFPWDAQEAALLIEQADQAMLRAKRDGKNRIYLVGGETDGEDERG"
     gene            complement(3918..6839)
                     /locus_tag="P5316_08965"
     CDS             complement(3918..6839)
                     /locus_tag="P5316_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85128.1"
                     /translation="MPFRLALFCLSGLLARCPMPVIFAADVPASQAPQPLTKPSQDLL
                     KQAQDLLVRGAYEEAAKNLEELAADPEQAVEAAIGLAEARRMVGRYADALAVLERVNA
                     VSSDSWQLARAEVLSEIGRYGQSIEAAGKAIELNSRNTSARRLLGHLYEITGDRLKAI
                     EAYAWFEALLRKSYPTTAVALTDSGIAIYRHAVLTRSPQTTARTKYVLQELLQPAVER
                     LDKEYWPALLASGELLLSKYNLEQAGDDFKAAIKINPNLAMAHVGLGTIALDQWDFEK
                     AAEEAAAALKINPHSPDAHCLQARIHLLERKDAEAVKAAAQALDTNPNHLEALGILAA
                     ARLLHGDKAGMQETVSRAEKVNPRNAIVPNEVASWLAMRNQFSEAEPLFKKAAELAPE
                     WADPLTGLGMLYMETGDEAAARRVLDSSWKLDPFNEKTFHTLNLLDKLEKFGHEETEH
                     FIVKSADGPESIIRPMVAAYAEEIYARVCDQYNYRPPRKTIIEVFPEHGSFAVRVTGR
                     PFLHTIGATTGPVIALDAPRSGPTSMTFNWPTVLRHEFVHTVTLAATGNRVPRWLTEG
                     LAQREEGRHWSWEWCQMLVMALRRGMLYDLNSIDWGFVRPKHHGGPMLAYAQSEWMVD
                     YIIERHGQGAIGTMLAGFRDAARQDEIVRNALGQSPEAFMRDFTAWASKQAESWGQPL
                     DPIPPIEEIDRRLVSAKDAEKVALLVSKAEALLDNHELDEAAKALRDARKINDRDARV
                     LTVMVQTLWAQAGQSDKKKEEKLRKECIKVAEELAKRDPDNPTACWVLGSQAIKDQDR
                     LRAVEMFARVVEARPRDLYSRSRLAELYIELKQPEKALAELIELARLDSNEESYPLRA
                     ARIHADAGRGAEAVEWYRKALMVAPYDKDTHEALGQLLMREQRFLEAIDAFRVVTQLK
                     PASAEAHSRLAFALYRAGRTEEAKGAAKRAVSLDPQSDAKSLLEDQPRSSSPSVSPPT
                     R"
     gene            complement(6985..8610)
                     /locus_tag="P5316_08970"
     CDS             complement(6985..8610)
                     /locus_tag="P5316_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85129.1"
                     /translation="MAILLAPVVLTHLILLAEGLSRMPAGHDGLKYRLPLVVTWLKAD
                     ALVMRPDTWSFSLPANGELILWWLLKGGFERVAGVAFGPASVLLGLAVWSIVRSLKGS
                     RCAAALAVAIPLSAHLVTFQVSHAYVDVFGTAFLASGVAAFFLAIGPERTLAARRVLV
                     TIAGLAIGIAMGTKPVNWIYALAFALIFFVVLVKRNRRNNDLAFLLPAFILACLACSA
                     FWFIRAGLLTGNPFYPVQVAVGDRVLLRGIRFSAYYEMYEQNDGSLSAMSQSPHQWLA
                     TIARIAGYAVTLNMLSGSVGPLFAMCVPIGMLVGVLMLLARGRRATRRNRLTIAVLTL
                     SSLALWTGPLQHYARFGIIYLLLATCMAMPVIGWAVRYWPRLTGLAFLTSTVLACALV
                     AGQPARELSRRLASGDFSRSGFYGLPPPVDQWPDGTRVINLSDWSGASTLTYPLYGRD
                     LKNDVIDYMTTKSLFPELKPTVEQLQELGVDFVFVREPFRRDWPTDPRLELVYDDSVQ
                     LPPSDPLPRSRIYRVPPPAAGENNGLVVANRPN"
     gene            complement(9383..10534)
                     /locus_tag="P5316_08975"
     CDS             complement(9383..10534)
                     /locus_tag="P5316_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85130.1"
                     /translation="MERRSSIFAFDTLTGLPRVTASLVLTLVFCAGFRAVLYAASDRL
                     DRIPAPAIFQRYIELEDQIISRAKTRPKIVLMGNSMARYGLLEDQIAAAAGMAPAEVA
                     NLGIEAGQPWDALFFWRRNPEFFSDVRLVVYNVGFSELQERSVRRRLGHFYRFSTLPE
                     KLTVDRWSDRMLMTLDWVWPYHSDRRDLVTWGLGLCGESDYLRPEAMRPAWEPSKMTT
                     LRARWLPRLESAKPPSAPGASVDPEWTPAEWPEGTSQCQVNLLTRLVDQWRHAGIKVL
                     LVAMPGATVIHRARFCNQDAQTRLAQFNAAMSAMTGGGVAYLCWRDGAEAGLDDAADF
                     MDECHLTPQGARKFTSLIVSRLTAMDWLPPPPQASSLAGNIGWYGSSDP"
     gene            complement(10503..11918)
                     /locus_tag="P5316_08980"
     CDS             complement(10503..11918)
                     /locus_tag="P5316_08980"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRR85131.1"
                     /translation="MLFNSFIFVGFLSVVLPLYYVLSHKWQNRMLLAASYVFYGAWDW
                     RFCFLLAGTTLVDFQVARAIDRTADERRRKLLLLLSLSFGLGVLGFFKYCGFFVDSLN
                     DLLACFGLGRMHWAVKVILPVGISFYTFQSLSYTIDVYRRRLKPVDGLVDYGLYVAYF
                     PQLVAGPIERAINLLPRIMTKRTVGPAQIATAAQLVLMGYLKKIAIADAVAPCVEQIF
                     SRPTACSSLKLLLGVYLFAVQIYCDFSGYSDIARGVSRWFGIELMVNFRQPYLSADIV
                     DFWRRWHISLSTWLRDYLYIPLGGNRCGRLKTYRNLMITMLLGGLWHGAAWTFVIWGG
                     LHGLYLSAAKLIGIRPMEQQIRTPRQLAAFGLRVLATFHLVCLGWVFFRAGSLDVAWS
                     YLTGLAQLTTAVELRLVCVTGFYLTLMLLIDLPCWVRNEEYPLTTRTRWYVRGLAQAT
                     AVILISFVGAGHGTAFIYFRF"
     gene            complement(11931..13355)
                     /locus_tag="P5316_08985"
     CDS             complement(11931..13355)
                     /locus_tag="P5316_08985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85132.1"
                     /translation="MPEVQTPIRQPGSFRDPSGFVFEFNGRILRAVDEDCLRTVRELL
                     DGGLLGRLIDEGLIVPTSLVESPDLLRRLKAVYPGQAGFLEHERIHPVSYPYEWSPSM
                     LADAGVCTIDLQIRLLEHGFSLKDATAYNIQFRHGRPVFIDVPSIERPPRLDVWIALG
                     QFGRMFTNPLLLNRYKGQSLRSCFLADLDGSDVSRVQRAFGRLERLSPRLLLDVTLPY
                     WLGRRASRLGDAGPRRLTCRQTTPAAQIINLRRLRSKLRNLAARNGSAGHWATYSETC
                     SYTDRAEESKVREVRRFLTEHRPASVLDVGSNTGRYSMLALEAGAKVVSIDSDQDCID
                     LLYRRIRHCGHPILPLCVDIANPSPAIGFRNRERASFLERVRADCVLALALIHHLHVS
                     ANLPVAGIRDMLADLCDRHLVLEFVPTDDVMFRKLMQFRVDVYQDFTLDACIRAFEER
                     FDVLRQVPVVDSRRTLLFMRKKDA"
     gene            complement(13419..13547)
                     /locus_tag="P5316_08990"
     CDS             complement(13419..13547)
                     /locus_tag="P5316_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85133.1"
                     /translation="MIAYIDPGSGTILLQLLIGSLIGAGLFFREGIGKLLRVFKRN"
     gene            complement(13766..15364)
                     /locus_tag="P5316_08995"
     CDS             complement(13766..15364)
                     /locus_tag="P5316_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR85134.1"
                     /translation="MASRFVASLMAVFILFGPYLSNSLGDLTRYLHMWQRSDMVLVLS
                     IVVVLAALATLADGLLRKLARPTLMRLYHHLFVLVVGAGILANVAFYGDKSQGWRIGR
                     TGMEMQTLWLILVAVVAYSFAKPASRLVFWCGRACLIASPVVPIVAFQLLAAPTYPLA
                     ADPLVSTSVVPASGGDAEVDRPPVYLFVFDEWSYERTFTEGAPKTAFPNLADLAARSV
                     VFHDAHSPGEDTATSMPALLFQTNLRPVVKNGSVGFDRDGRVVPRSDLECLFSVVADQ
                     GYRRIMIGAFLPYQAWLGDQVDICRSYCYYPRADGMLSWVTIHGFNAVSYLTDPWSVL
                     AYAKLKTRMNDRQILTVHERTRNDAFSVIRDQPAAAFAVIHYMLPHEPFILDPDGTYR
                     GPDDSAWIRPNVEGYTRNLEGLDRLIGEFVQAMHGAGRFDDALVIVTSDHSWRFDPAR
                     KSGTIVTPVTHVPLLIKLPGQQTPVSISNRFETRRLGSLIRWVIASPPSPGQIESLVR
                     QWVEGNAQRDAVTWVVPRPPVCGR"
     gene            complement(15480..16538)
                     /locus_tag="P5316_09000"
     CDS             complement(15480..16538)
                     /locus_tag="P5316_09000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR85135.1"
                     /translation="MALECSIVIPLCNEEQSLPTLHQRLSAVMRRLAIPYEIIYVDDG
                     SRDRTAEVIRDLHVQDSTVKGVFLSRNFGHQAALCAGLEAATGRSVITMDGDLQDPPE
                     VIPDLIEKWRSGYQVVFARRRSRRENIFKKAAYFSFYRLLRQMSEIAIPLDTGDFALM
                     DRRALDEMNSLPERTRFLRGLRSWVGFRQAEIEYDREPRHTGETKYTFRRLLRLAMDG
                     ILSFSDAPLKAITLTGGLISAIALSILAVSVSGLWSAASGKGVLTLGAALSLLGGIQL
                     IGLGVVGEYMSRIYREVRGRPMYVAKERLGFQRMPRPVPDVLDFLSTDVRQRRQRVTA
                     ARLSPSRSMERSGHENSA"
     gene            16773..18707
                     /locus_tag="P5316_09005"
     CDS             16773..18707
                     /locus_tag="P5316_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85136.1"
                     /translation="MITATRYMHLRSSDMAPPLAGIRAGTFAAVTVFIIVWVISLSRS
                     VFDPIGFDQALYQYITDRVMLGQRLYADVWDQNTPGIIGIHWLATILVGRTPIALRVF
                     DAFWQCATITALVALAVRDGRRWQAGWLAATLYVLAYYGLGYVHTAQREGFAVLPLLL
                     AVHALAPATGHDRASRAPAPRHVLAGALCFCVFAIKPPLGLCFGMLWLMILVRWLAPL
                     LRPAAPSLQNGASPLQQLCPGAALGETRHESRFSITRLSESAEKRALFSLSLGFIVSA
                     ALAVALVHQLGWWSGLWRTLARQDVAGYIRGPQLIRESAPYLFTGAVGIAGIALLAHV
                     LRAKSHAGSSLRLRDLAAPVTGGLAVFALLLTIQRWPDWQQAFLKTAGILVPAGGAML
                     LAQWGRRSRIWQMTALLGLASLAAVLWQGHFALYQFPPLLACGAYIAAAEISERFPRF
                     CEMDKAGRVWTVVCLGAVMHLAAGTWGWTMTFYGRSPYVLSGTTLMQHYTKVTANKPS
                     FPTYETTSAAAVRVRELTGETDPIVCLVDEPRLYYLAQRPPAYPLLRTQVCYSSMFPA
                     LFEAIRTQHPKVLLARLPVGARGAGDARAAEAAVLADLEGYFGPPARVVRDEYRLTEV
                     VNGDLCILQPQARISSNADR"
     gene            complement(18685..20151)
                     /locus_tag="P5316_09010"
     CDS             complement(18685..20151)
                     /locus_tag="P5316_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85137.1"
                     /translation="MAAKSSDYHMAILLVSLLLASSRIVGAVEIVVDNSDSGFTILSG
                     TWMTGTSPTPYGTDYRWRATVAGSPTGEVEWRPGLPASGTYEVFVWYVEGANRATNAP
                     FVIHGTGDPVTVFVNQQTNGSQWFSLGSYSFAAGTGGYVRLNNVATGDVVIADAVRFV
                     LAGDMPVNTDFDGDQDVDGSDLAIFLACITGTDAGPVADACHWADFDGDLDVDQADFG
                     VLQHCLSGPDVPATSGCLSSPIPPRPAGAVTGSQFVQQVWSIPKATREQMILAEMTAG
                     NIPEFLRRFVPVTVTATIASVEHTATWFVMPDYLAIGSDADFVRFPMGPLTAQSIGDT
                     FQCMMPTRKMVNDIWSAAPCKLAPHPYSPSVYNIESVEVFNMSNTYIEGQRIAAGTSL
                     GTLIAGTKKDVVVTAQLATHPGKVAIYGWHQLNGQPIQPLYLGHTITYMDYSHGIRLV
                     KRVMLVDGRPMLVEDVLKDPVLSVLISDEGVVNDPRYY"
     gene            20367..21023
                     /locus_tag="P5316_09015"
     CDS             20367..21023
                     /locus_tag="P5316_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85138.1"
                     /translation="MEIYNTHSDVKDESVKMLLAAMILNLPAVRAHSEAAFLSFFDYP
                     AEFLARFDILRLESPFAGIVANDSHQNQGFKVMAMPDGGLEVFDAAEESVFRSDGVVA
                     RMLLAALGCTKTPEQTLQLSRIQLAPYEISVRHVGTFLQIDHIDEKTVRHALRTGRIV
                     LGFEIIASPAGRRFLGGAGRHTGRNSRRSDPVAAEAGAPVGPSSGRRGPEPPQRASIS
                     "
     gene            21222..>21976
                     /locus_tag="P5316_09020"
     CDS             21222..>21976
                     /locus_tag="P5316_09020"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HRR85139.1"
                     /translation="MRKSMCAIVIVLPTVLCALARADLAGYIDKKDATYSWTTVSTSE
                     SDPESPIRYAELRMVSQTWRGIEWKHRMAVVCPLKPQTTSHALLIISGGSWREGQEQP
                     KLDKDSREFKLATTIAGACGMPVAFVMHVPFQPMFGGLKEDQIISKTFDEYLKTGDEE
                     WPLLLPMTKAAVRAMDTAQDFAKKEWRIDISKFIVTGASKRGWTTWLTGASDKRVVAI
                     APMVIDVLNMAEQMKHQKETYGTYSEEIDDYTRL"
BASE COUNT         4488 a         6841 c         6626 g         4021 t
ORIGIN      
        1 ccctggcgct atcgccaagg ccattcttgc ctcgaaagtg ttcaacttgg gttagtccct
       61 tccgtcgcga cgggtgcgca ctttgcgcgc atgtttctgg atggcagtgc gtttgggttg
      121 cggcccgcaa aagggcgctt cgccttggga gttgaacccg gaatgcatcc tacttgaacg
      181 ccgcgagtct tttgcagatt tcttcttcga gccttcgggc cgctgtggaa ccatctttac
      241 tcttgttggt gtcgttgcag agaatgctga atgcaaagta tcgcttgcgg tcggcggcca
      301 caacgtagcc gctaagggca ctgaccccgg caatgtagcc ggtcttggcg tacaccgttc
      361 ccttcagcgc cggcatgcga ttgcgcaacg tgccatcagg gtcgcccggc gttgcgaaac
      421 cggcccacca ctgcttgcgg atcggcatct gccggtcgac atacaccagc gtctcgcaaa
      481 gcgaggtcgg cgagatgcgg ctgttgtggc tcagacctga gccgtcgtca attacaaacc
      541 catcggtctt aacacccagc ttcgcgagga attcccggat cactgctgcg ccggcggcac
      601 gcgtccccac cgccttcccg ccggtcgaat aggctccgag cgtcttgagc agcgcttcgg
      661 cgaacacgtt caggctgctc ttattcatcc gccagaggaa atcaatcggc ttgctctcgt
      721 acgcggcgac gatcttgaac tccgccggga gcgagccgtc ggcctggcga acgcgctgcc
      781 gacgaacctt gccgtcgatc ttgatgcctt tggcggacag caccgaatga accgtccggg
      841 cgaagaaatc tcccggatcg ccgacgggaa tcgaggtcgg gttaccgtca cccgtcggcc
      901 gggacacggt tttgctcacc gagatagtca gcggatcaac cgaggcacgg cgaatcacag
      961 gctggccctt gctgccggtc accgacttat tgtccacctt cacccaatcg ggaaccagct
     1021 ctacaatcgc gggcttgcct ttctcagcgg gcgtcaatat cacatccacg cagttatcat
     1081 tgaagttgag tccgcccacc ggggcggtaa accagtcttc caggttatgc tgctgtcgcc
     1141 aactggcggg cacgaactcg gtgtcaaaga tcgaatcgtc gaagatcaaa tcgccctcga
     1201 cggcatggat gccttcagct ttcatccggt cggcccaacg ttcaaagact gcggtgaccg
     1261 gctgccgctg ggccttggcc agcttggggt caccgatcga cgggtcgccc gccccgatga
     1321 tcaccaggtc gcgccccagc attcccagca atgtgcgata ctggaaatcg ggcggcaaca
     1381 tggtcagggc agcggccgtg gtcagaattt tctggttact ggccggcttg aagagctcgt
     1441 cggcgttctg ggcatacacg agttgcggct gctcgatgcc cagagcaacg atgcgaattc
     1501 ccactttgac cttggccagg ctcggggccg cagccagtgc cttgatctca tcgccaagag
     1561 cctcgtacgg accggcccag gcaacccgac cccaaacgca gacggacaac aggccgataa
     1621 tcgccgaacg aacgtgtatg ctcctgaatc gcatgaacat ctccatcaag ctcccggagg
     1681 ccgttttcgg tgctctgccg accggctgga agccggtccc acgcaggcct tgggacggac
     1741 ttcgtgtaag tgttgaggtc gactccaagc gggccgatat cccagtgttt gaccgcgcca
     1801 ttctccaacg gtaaactaga taaatagcgg cagagagtca atctggcaat gcgattcagg
     1861 cgagggcggg gcaatgtcac gatttcagcc gcacggtgcg cgaatccttt gcgtcgccgg
     1921 cagcgagggt gagttccctg aggccgtccg gcaatggctg gcccaagtga cgctgcgcac
     1981 cgagtactgc agcgatgtgt acgatgcgtt ggccgttctt gcgaccggaa cacgtcccat
     2041 ccttgtgatc gtgggcatcg actccgtcga ttggagtgag atggatttct tcagccatgt
     2101 tgccgctctc agccgcggca cacgcatcta tgtcgcggcc cacgatgcca gcgacggccg
     2161 ggtgttcgcg gccggagaac ggggagcccg tctgttcgat cccgcatcgg caaacgcgga
     2221 cattgaggcg gtcttcaccc aggaccaggc aagcggaccg ggcggtttgc tggccggaac
     2281 gctgcgacct tctcaggtca atcgccagcc ggtcaatgtg ccgcccgcaa ccgcagagca
     2341 aaaccagcct cctgcgtccg agaccttcaa gaccaagacc gaggagccgc cgatccggct
     2401 ggtcaccgct gccgagcccg acgaaaacag cgacgttgag ctatctgtgc cgtgggcgcc
     2461 tcatcccagt cggcccaggc gtaccccgcc caaaggcccg gcagatgcgc cgcagccatc
     2521 accgcgccaa accgacggag gcatcgaaac agacaagccg ttgccccgcg tcgagctgac
     2581 gcccgatgag attgccgccc tgctgggcaa gccgggctcg tcgccacggt tgagggaaca
     2641 gggatcatga cgccggaccc cctcattccg actcgcgagc aactgctgat cgtgcacgat
     2701 ccggctgatc tggcggcgtt tgtccgcgga cgctacccac agttcgacgt ggccactgct
     2761 cctggataca tggatggtct ggtcgctctg gcaaggcagt cgccccgcgg cgtgctcgtg
     2821 ggcgtggagc cgaccgtccg caagctgagc aacgcgattg ccgcacttcg caaggccgcc
     2881 ggaccctcgt cgcgtattgt gctgtgctgc atgcccagcg gcgagccggt ggcccgccgc
     2941 gtggtcgccg ccggtgctga tgattacctg atatatcccc ctgaaggtgc agaacttgac
     3001 gccgctctag cgttgcccac gcccggaggg gatgcccgcc tcgcgacgcc tgagccgccg
     3061 gttcccacct ggtacgaact gcattcgctg accgacatcc tggcggggct ggccgacggc
     3121 cggacggcga tgctggaacg gctctgccgc ctcctggccg acgcgatgcg gacaccgttt
     3181 gtgcgaatca ccttggaaag agaaagcagg catgtcggcg atcccaagac cgagccggcg
     3241 ctcttcgaga cgattcagtc gggcggccag gttctcggca agattgaagt cggcccgcga
     3301 catcgcggtc cgttctcggc cgccgaggtt gagaagcttc ggcatcacgg ccggctgatc
     3361 gcccatctgc tcaaggccgc cgaacagcaa caacagtggc atcgcctggc catgatcgac
     3421 gaagtgacgc aactgcccaa ccgccgatac ttcaggcagg cactggaaac actcatccgc
     3481 cgggccgcga cggagcgatt ctcggtgacg gtggtcatct tcgatctcga cggcttcaaa
     3541 cacttcaatg acacctacgg ccacaacgcc ggcgaccagg tactccgcga gctggcccaa
     3601 ctgatccgcc gccactgccg acggcacgat atcgtcgccc gttatgcggg cgatgagttc
     3661 gtcgttgcct tctgggatgc cgaagaacca cgcgtggccg gctccaagca ccctaccgac
     3721 gccctggcgg tgctgcggcg gttccagaaa acgcttgaat cgcaggagtt cgccaacctg
     3781 ggtctggaca gcaaaggccg gataaccatc agcggcggcc tggccacttt tccatgggat
     3841 gctcaagaag ccgccctgct catcgagcag gccgaccagg cgatgcttcg agccaagcga
     3901 gacggcaaga accgtattta cctcgtcggg ggtgagacag acggtgagga tgaacggggc
     3961 tgatcttcga gcagtgactt ggcgtcggat tgcggatcga ggctgacggc ccgcttcgca
     4021 gcacccttgg cctcctccgt cctgcccgca cgatatagtg cgaacgccag tcggctgtgg
     4081 gcctcggcgg aggccggttt gagctgggtc actacgcgga aggcgtcaat cgcttcgagg
     4141 aaacgctgtt ctctcatgag caactggccg agggcctcat gcgtatcctt gtcatacggg
     4201 gccaccatca gggccttgcg ataccactcg acggcctcgg ctcccctgcc ggcatcagca
     4261 tggatgcggg cggcgcgcaa cggatagctc tcttcgttcg agtcaaggcg ggccagctcg
     4321 atcagttcgg ccagggcctt ctccggttgc tttagctcga tatacaactc ggcgagccga
     4381 gaacgcgaat acaaatccct cggacgggcc tcaacaactc tggcgaacat ctcgacggct
     4441 ctcaagcgat cctggtcctt gatggcctga ctgccgagca cccagcaggc ggtgggattg
     4501 tcgggatccc gcttggcgag ctcctcggca acctttatgc actccttgcg cagcttctcc
     4561 tccttcttct tgtcactctg accagcctgg gcccacaacg tctgcaccat gacggtgagc
     4621 acgcgtgcgt cccggtcatt gatcttccgt gcatcgcgaa gggccttggc cgcctcgtca
     4681 agttcatgat tatcgagcag agcttcagct ttcgaaacca ggagtgcgac cttctcggcg
     4741 tccttggcgc tgacgagcct cctgtcgatt tcctcaatgg gaggtatggg atccagcggc
     4801 tgaccccacg attccgcctg cttgcttgcc caggcggtaa agtccctcat gaatgcctcg
     4861 ggggattggc ccaaggcatt ccgcacgatc tcatcctgcc gggcggcatc gcgaaagccc
     4921 gccagcatgg tcccaatggc gccttgtccg tgacgctcga tgatgtagtc caccatccac
     4981 tcgctttggg cataggccag catcggcccg ccgtggtgtt tcggccgaac gaagccccaa
     5041 tcgatcgagt taaggtcgta gagcattccc ctgcgcaggg ccatgaccag catctggcac
     5101 cattcccagg accagtgtct gccctcttcg cgctgggcca acccttcagt cagccaccgg
     5161 ggaacccgat tccccgttgc cgccagggta accgtgtgaa cgaattcatg ccgcagcacg
     5221 gtgggccaat tgaaggtcat gctcgtgggg ccgcttcgcg gggcatccag ggcgatgacc
     5281 gggccggtgg tcgcgccgat cgtgtgcagg aacggccggc cggtgaccct cacggcgaaa
     5341 ctgccgtgct cggggaagac ctcaatgatc gtcttgcgcg gcggacggta gttgtattga
     5401 tcgcagacgc gagcgtaaat ctcctcagca taagcggcca ccatgggtct gatgattgac
     5461 tctgggccat cggcggattt gacgatgaag tgttcggtct cctcgtggcc gaacttctcg
     5521 agcttatcca gaagattcag cgtgtggaag gttttctcgt tgaacgggtc aagtttccac
     5581 gacgaatcga gcacccgccg ggcggccgct tcgtcgccgg tttccatgta gagcatgccc
     5641 agcccggtca gcggatcggc ccactcagga gccagctcgg ccgccttttt gaacaacggc
     5701 tcggcctcgg aaaactggtt tcgcatggcc aaccaggaag cgacctcgtt gggcacgatg
     5761 gcgttgcggg gattgacctt ctcggcacgg ctcacggtct cctgcatgcc cgctttgtcg
     5821 ccgtgaagga ggcgcgcggc cgcgaggatc ccgagggctt cgaggtgatt cgggttcgtg
     5881 tccagtgctt gggcggcggc tttgacggcc tcagcatcct ttcgttccag gaggtgaata
     5941 cgagcctgca ggcaatgcgc atcggggctg tgcggattga tcttcagggc agcggcggcc
     6001 tcttcagcgg ctttttcaaa atcccactga tccaaagcga tggttcctaa cccgacgtgt
     6061 gccatcgcca ggttggggtt gatcttgatg gcggctttga aatcgtcgcc ggcttgctcc
     6121 aggttgtact tgctcaacag cagctcgccg gacgccagca gcgccggcca gtactccttg
     6181 tccaaacgct ccacggccgg ttgcagcagc tcctgcaaaa cgtacttcgt ccgggcggtg
     6241 gtctgaggac tgcgagtgag aaccgcatga cggtagatcg caatacccga atcggtcagg
     6301 gcaacggccg tggtcgggta gcttttccgc aggagggctt caaaccacgc gtaggcttca
     6361 atcgccttga gtctgtcgcc cgtgatctcg tacaaatgtc ccagcagtcg gcgggcgctg
     6421 gtgttgcgac tattaagctc aatagccttg ccggccgctt cgatggactg tccgtaacgg
     6481 ccgatctccg acagaacctc tgcgcgagcc aactgccagc tatcggagga tacggcgttg
     6541 actctttcga gaacggctaa agcatcggcg taccggccca ccattcggcg ggcttcggcc
     6601 agcccgatgg ctgcttcgac ggcctgctcc ggatctgccg ccagctcctc caaattcttg
     6661 gccgcttctt cataagcgcc cctgacgagg aggtcctgag cctgtttcag aaggtcttgc
     6721 gacggcttgg tcagcggctg gggcgcctgg ctagcaggaa cgtcggctgc aaagatgacc
     6781 ggcattgggc accgagccag aagacctgaa agacaaaaca gggccagacg aaaaggcata
     6841 tcgcgcggtc tcatacgcac attctaccga tacagccgac gcactcgcag gccgtcgtca
     6901 caacacggcc accacgttat tgtagcacaa cgggatgtcc gctttcagca taagaaggga
     6961 tatgactgga ctgcggtgag gcactcaatt cggcctgttg gcaacaacaa gcccgttgtt
     7021 ttctccggca gcaggcggcg gcacacggta gatgcgggaa cgtggtagcg ggtcacttgg
     7081 cggaagttga accgaatcgt cgtacaccaa ttccagacgc ggatccgtcg gccagtcacg
     7141 gcgaaaaggc tcgcgaacga atacgaaatc aacgcccagc tcctgaagct gctccacggt
     7201 cggtttcagc tccggaaaca gcgatttcgt cgtcatgtaa tcgattacgt cattcttcag
     7261 atcgcgacca tacaggggat aggtcagagt gctggccccg ctccagtcag aaaggttgat
     7321 cacgcgcgtg ccgtcgggcc actgatcgac gggcggcggc aggccataga agccggatcg
     7381 cgagaaatcc ccggaagcca gacgcctgct gagttcgcga gccggctgcc cggcaaccag
     7441 agcgcacgca agtaccgtcg acgtgagaaa ggccaaaccg gtcaatcggg gccagtatcg
     7501 caccgcccag ccgatgaccg gcatggccat gcacgtcgcc aggaggagat agatgatgcc
     7561 aaatcttgcg tagtgctgca gcggcccggt ccacagggca agggacgaca acgtcagcac
     7621 cgctatcgtc agccggtttc gccgggttgc tcgcctgccc cgggcaagca gcatcaacac
     7681 ccccacaagc atcccgattg gcacgcacat ggcaaacagc gggccgacgc tgcccgagag
     7741 catgttaaga gtcaccgcat atccggcgat tctggctatc gtcgcaagcc actgatgtgg
     7801 cgattgagac atcgccgaga gtgaaccgtc gttctgttca tacatctcgt agtatgcgct
     7861 gaacctgatc cccctcagca gcacgcgatc gccgacggca acctgtaccg gatagaaagg
     7921 attgcccgtc aacaggcccg cccggatgaa ccagaaagcc gagcatgcca ggcaagccag
     7981 tatgaacgcc ggtagtagaa acgcaagatc gttgttccgc cggtttcgct tcacaaggac
     8041 aacgaagaag atgagagcaa aggccaacgc atagatccag ttgaccggct tggtacccat
     8101 ggcgatgccg atggccagcc ccgcgatagt cacaagaacc cttcgagccg ccaaagtacg
     8161 ttcgggacca atcgcaagaa aaaacgccgc gacgccgctt gccagaaacg cagtcccgaa
     8221 gacatcgacg taggcatgtg atacctggaa ggtgaccaag tgggcggaca gcggaatcgc
     8281 cacggccaag gcggccgcac atcgcgaccc cttcagggat cggacgatcg accagactgc
     8341 cagtcccagg aggaccgagg ccggcccgaa ggccacgccg gcgacacgct cgaagccccc
     8401 cttcagcagc caccagagaa tcagttcacc gttggccggg agcgaaaagg accaagtatc
     8461 cggtcgcatc accagagcgt ccgctttcag ccaggtgact accagaggca gacgatactt
     8521 cagaccgtcg tggcccgccg gcattctgga gagtccctca gcaaggagaa tcaaatgagt
     8581 gaggacaacc ggcgccagaa gaatcgccac cgaccacgcg ttccgggaac gagcgcccgt
     8641 ttcggttggc ggcctgcgcc tgctccaagg gtaccggtat cggcttgtcc gccggtaccc
     8701 aactgacgcg gaaaagacgt cgccaggtgt tgccagaggc gacctcaccg ccgtaggtga
     8761 ttggaccgga tatgcgagga cgaaccaggt taaggccaag atcaccacga acgccgctgt
     8821 gatgagggcc ggggacagac ctgacagcaa tccaagcaaa cggaggaatc cgacgaggtg
     8881 aaaggctacg atccagcccg aggttacgag cacggccgac agaagtgctg ccgtaggctt
     8941 gtcggcatcc ccaatccacc gctgccgcag caaccccccg agcgcgagga aacacgggat
     9001 cgcgatggct ggagcagcga ccagccacgt gatcgtctcc ggagtgggtt tcatagatgc
     9061 cgactccgag cttggatgtc gacggtcatg gccacagcca acctgccacc atccaataga
     9121 attgccggac ctcgcgagat cgcagcacaa accccaccag aaccatgata gacgcggcca
     9181 caatcacgcc tgagacgatc cggcaacgcc agaatctgct cgttgcccga caatggctct
     9241 cgccgttgcg catgcgaaat tgatcggcgg caacaatacc ggacttcata cttatccggc
     9301 tgcgcatcac gatcgtccga taacctcttt gctgttggat ccgcccgaca tctcggcctg
     9361 atatcgcagc caagcctgtc ttctacggat ccgaagagcc gtaccaaccg atgttgccgg
     9421 ccagcgacga cgcttgaggc ggcgggggca accaatccat ggccgtcagt cttgacacga
     9481 tgagcgaggt gaacttgcgt gccccctgcg gggtgaggtg acattcgtcc atgaagtcgg
     9541 cagcgtcatc caggccggct tcggctccgt ctcgccagca caggtaggca acaccaccgc
     9601 cggtcatggc ggacattgct gcgttgaact gagccaggcg cgtctgtgca tcctgattgc
     9661 agaacctggc ccgatggatg acggtcgcgc caggcatggc caccaatagg accttgatcc
     9721 ccgcgtgtcg ccactgatca accagtcgcg tgagaagatt gacctggcac tgcgatgttc
     9781 cctcgggcca ctcggcgggc gtccattcag gatcgacgga cgcgcccggc gcagagggcg
     9841 gcttcgcgct ctcaagtcgc gggagccaac gggcccgtaa cgtggtcatt ttggacggct
     9901 cccatgcggg gcgcatcgct tccggccgga gataatcgga ttcgccgcag agccccagac
     9961 cccatgtcac cagatcgcgg cgatccgaat ggtaaggcca gacccaatcc agcgtcatga
    10021 gcatacgatc cgaccagcga tccacagtga gcttctcggg aagcgtgctg aatcggtaga
    10081 aatggccaag tctgcgtcga accgaccttt cttgcagttc cgagaatccc acgttgtaga
    10141 caaccagccg aacgtcgctg aagaactccg gatttcgacg ccagaagaat aaggcgtccc
    10201 aaggctgacc cgcctcaatg ccgagattcg ccacctcagc aggggccata ccggccgccg
    10261 cggcgatctg atcttcgagc aggccgtagc gggccatcga attgcccata agcacgatct
    10321 tcgggcgcgt tttggctcgg ctgatgatct gatcctccag ctcgatgtat cgctggaaga
    10381 tggccggggc gggaatgcga tcaagccggt cggatgcggc gtagagtacc gcgcggaacc
    10441 ctgcgcaaaa caccagcgtc agcaccagcg aggccgttac gcgtggcagg ccggtgaggg
    10501 tgtcaaaagc gaaaatagat gaacgccgtt ccatgaccgg ctccgacaaa ggaaatcaga
    10561 atcacggccg tcgcctgtgc caacccgcgg acataccagc gagtccgggt ggtcaaggga
    10621 tactcctcgt tcctcaccca gcaaggcaga tcaatcaaca gcatcagagt caggtagaac
    10681 cccgtgacgc aaaccaaccg cagctccacg gccgtcgtca gttgagcaag tccagtcaga
    10741 taactccacg ccacgtccaa gctgccggcc cggaaaaaca cccagcccag acacacaaga
    10801 tggaaggtcg ccagaacacg cagcccgaac gctgcgagtt ggcgcggcgt acggatctgc
    10861 tgctccattg ggcgaatccc gatcagcttg gctgcgctga gatagagccc gtgcagacct
    10921 ccccagatga cgaaggtcca cgcggccccg tgccacaacc cccccagcaa catggtgatc
    10981 atcaggttgc ggtaggtttt cagccggcca caacgattac cacccaacgg gatgtaaagg
    11041 taatcgcgca accaggtgga aagcgaaatg tgccagcggc gccagaagtc gacgatgtcg
    11101 gccgacagat acggctgccg gaagttgacc atcaactcga taccaaacca tcggctcacg
    11161 ccgcgggcga tgtcgctgta tccggaaaag tcgcagtaga tctggacggc gaagagataa
    11221 acgccgagaa gcagcttcag cgaggagcac gcggtcggcc ggctgaagat ttgttcgacg
    11281 cagggggcca cggcgtcggc gatggcaatc tttttgagat aacccatgag cacgagctgc
    11341 gcagcggtgg cgatttgcgc gggaccgacg gtacgctttg tcatgatccg cggcagcagg
    11401 ttgatcgccc gctcaatcgg cccggccaca agctgcggaa agtaggcgac gtacaggccg
    11461 taatccacga gaccatcaac gggcttgagc cggcgacgat atacgtcgat cgtgtagctg
    11521 agggactgga aagtatagaa actgatgccg acggggagaa tgaccttgac agcccagtgc
    11581 atccggccca gtccgaagca ggccagcaga tcgttcaggc tgtccacaaa gaagccgcag
    11641 tatttgaaga atcccaggac acccagcccg aacgagaggc tcagcaggag cagcagtttt
    11701 ctacgacgtt cgtcggcggt acgatcgatc gctcgggcaa cctgaaagtc gaccagcgtc
    11761 gtacccgcga gcaggaaaca gaaacgccag tcccaggctc cgtagaacac gtagctggcc
    11821 gccagcagca tgcggttctg ccacttatgc gacaagacgt agtacagcgg cagtacgacc
    11881 gacaggaagc caacgaatat gaagctgtta aacagcatga gacttcaacg tcatgcgtcc
    11941 ttcttgcgca tgaacagcag cgtccgccgg gaatccacga cgggaacctg ccgcaagacg
    12001 tcaaatcgct cctcgaaagc ccggatacaa gcgtcgagcg tgaaatcctg gtacacatcc
    12061 acgcgaaact gcatgagctt gcggaacatc acgtcgtcgg tgggtacgaa ttcgagaacc
    12121 aaatgccgat cgcacagatc ggccagcatg tctcgaatcc cggctacggg cagattagcg
    12181 ctgacgtgca agtgatgaat gagtgccaag gccaggacgc aatccgcccg gacacgttcg
    12241 aggaagctcg ctcgctcgcg attgcggaag ccgatcgcag ggctgggatt ggcgatatcc
    12301 acgcaaaggg gaagaatcgg atgtccgcag tggcggatcc tgcgatacag caagtcgatg
    12361 caatcctgat cgctgtcgat cgagacgacc tttgcgccgg cctcgagtgc cagcatcgag
    12421 tagcgaccgg tgttgctgcc gacgtcaagg acggacgcgg gccggtgttc cgtcaggaaa
    12481 cgccggacct cgcgaacctt ggattcttcg gcacggtcgg tgtagctgca cgtctcgctg
    12541 tacgtcgccc agtggccggc agaaccgttc ctggcagcca ggtttctgag cttggaccgc
    12601 agccggcgaa gattgatgat ctgtgcggcg ggcgtcgttt gcctgcacgt gagccgacgc
    12661 ggcccggcgt cgcccaacct cgacgcccgg cggcccaacc agtaaggcag cgtcacatcc
    12721 agcagtagcc gcgggcttag ccgttccagc ctgccgaagg cccgttgcac gcggctgacg
    12781 tcacttccgt caagatcggc gaggaaacat gatcgtaagc tctgcccctt atagcggttt
    12841 aataacaagg gattcgtgaa catgcgcccg aactggccaa gggcaatcca aacgtccaat
    12901 cgtggcggac gttcgatcga gggaacatcg atgaacaccg gccggccgtg gcgaaactga
    12961 atattgtagg cagtggcatc cttcagcgag aaaccgtgtt ccagcagccg aatctgcaag
    13021 tcgatagtgc agacgccggc atcggccagc atggacgggg accactcgta cgggtagctg
    13081 acaggatgaa tacgttcatg ttcgaggaag cctgcctgcc ctgggtaaac ggccttcaat
    13141 cgccgcagga gatcgggcga ttcaacaagc gaggtgggga cgatcaaccc ttcatcgatc
    13201 agtcgaccaa gcaggccgcc gtcgagaagt tctcgaacag tccgcagaca gtcttcgtcg
    13261 acggcgcgca gaattcgacc gttgaattcg aacacgaacc cgctgggatc gcgaaatgat
    13321 cccggctgcc tgattggtgt ttgaacttcc ggcatccgct ctcccgagca ttcatgagtc
    13381 agcgttccgc agcaccgatg agtaggccga ccaaaccgtc agttccgctt gaagacgcgc
    13441 agcagcttcc cgatgccttc gcggaagaag agccccgcgc cgatcagcga accgatcagc
    13501 aactgcagga gaatcgttcc ggaacctgga tctatgtaag cgatcatcgg ccggcaccgc
    13561 ctcaagacac ctctgccggg acatcggtcg gaatcggctt gcggccctgc ccgcaccgag
    13621 cccctatcga ggattactat cgttcatccg ataagccaac tgaacatctt tccctcagac
    13681 cggcccccag cctccgcaga cccgcaaagc caacccggca gtactgggaa agggctgcct
    13741 tcacgcctcc gcccttcccg gcggttcacc ggccgcacac gggcgggcgc ggaaccaccc
    13801 acgtcacggc atcgcgttgc gcgtttccct cgacccattg cctgacaaga ctctcgatct
    13861 gtcccggcga cggaggggaa gcgattaccc atcgaatcag tgaaccgagg cggcgggttt
    13921 caaatcgatt cgagatcgaa accggcgttt gctgaccggg aagtttgata agcaacggca
    13981 cgtgggtgac cggagtgacg atcgtaccgg actttctggc cggatcgaac cgccaggaat
    14041 gatccgaagt cacaatgacg agcgcatcgt cgaaccgtcc cgcgccatgc atggcctgca
    14101 cgaactcacc gatcaggcga tcgagcccct ccaagttcct cgtgtatccc tcgacgttcg
    14161 gccgaatcca cgccgagtcg tcgggcccac gataggtgcc gtccggatcg aggatgaacg
    14221 gttcatgggg aagcatgtag tggatcaccg cgaacgcggc ggccggctgg tctcggatga
    14281 cggagaaggc gtcgttccgc gtccgctcgt gcaccgtgag aatctgccgg tcgttcatgc
    14341 gggtcttgag tttggcgtaa gccagaacgc tccacgggtc cgtcaagtat gagaccgcgt
    14401 tgaaaccgtg gatcgtcacc cagctcagca ttccatccgc tcgcgggtag tagcagtagc
    14461 ttcgacagat atcgacctga tcgccgagcc acgcttggta tgggagaaac gccccgatca
    14521 taatgcgtcg atagccttgg tcggcaacca ctgagaacag gcattccaag tcggacctcg
    14581 ggaccacgcg gccatcgcgg tcaaagccga cactgccgtt cttcacgacg ggccgaaggt
    14641 tcgtctgaaa caacagggcg ggcatcgagg ttgcggtatc ttcgcccggg gaatgagcat
    14701 catgaaagac aacggaccgc gcggccagat cagccagatt gggaaaagcc gtcttcggag
    14761 cgccctccgt gaaggtccgc tcataggacc actcgtcgaa aacgaacaga taaaccggcg
    14821 gcctgtcgac ctcagcgtcg ccgcccgacg ccggcacgac cgaggttgaa accagcggat
    14881 cggcggccaa tggatacgtc ggggccgcca gcagttgaaa cgccacgatc ggcaccacgg
    14941 gtgatgcgat gaggcacgcc cggccgcacc agaaaaccag cctcgaagcc ggcttggcga
    15001 aggaatatgc cactacggcc accaggatca accagagcgt ttgcatctcc atgcccgtcc
    15061 ggccgatgcg ccaaccttgc gacttgtcgc cgtagaaagc cacattcgcc aagatgcctg
    15121 cgccaacaac aagcacaaaa aggtgatggt agaggcgcat cagggtcggc ctggcgagct
    15181 tgcgaagcag cccatcagca agcgtggcaa gagccgccag tacgacgacg atgctcagca
    15241 ccagaaccat gtcgctgcgc tgccacatgt gcagataccg cgtgagatcg ccaagggagt
    15301 tggacaggta gggaccaaag aggataaaca cggccatcag cgaggcgaca aaccgtgagg
    15361 ccaccggcct cagtttcgtc cagcttctcg gtgagccatg catcggaaca cccgccgctt
    15421 ccctcctctg agttgcatcg atcagggact tccagcggtt tctcgcctcc atccggcgat
    15481 caggcgctgt tttcgtgacc agatctctcc attgacctgg acggcgacaa acgtgcggcc
    15541 gtaacacgct gtcgacgctg cctgacgtcc gtgctcagga agtcaagcac atccggcacc
    15601 ggccggggca tccgctgaaa accaaggcgt tctttcgcta cgtacatggg ccgtccgcga
    15661 acctcgcgat agatgcggga catgtactct ccgaccacgc ccagtccgat aagctgaatg
    15721 cctcccagta gactcagagc agcgcccagt gtgagcacgc cttttccact tgccgccgac
    15781 cacaaacccg acacgctgac cgccaggatt gacagggcga tcgcggaaat gagaccgccg
    15841 gtcaaggtga tggccttcag cggcgcatcg gagaatgaca gaatgccatc catcgccaga
    15901 cgaagcaaac ggcgaaaggt gtatttggtt tcgcccgtgt gccgaggttc acggtcgtat
    15961 tcgatctccg cctgccggaa accgacccag cttcgcagtc cgcgaaggaa tcgcgttctt
    16021 tcgggaaggc tgttcatttc gtccagggct cggcgatcca tgagtgcgaa gtcacccgtg
    16081 tccagcggaa tcgcaatttc gctcatctgt cgcaacaatc ggtagaagct gaagtaagcg
    16141 gccttcttga aaatgttctc gcggcgactg cgacgccgtg caaaaacgac ctgatatccg
    16201 cttctccatt tttcgatcag gtccgggatc acctccggcg gatcctgtaa atcgccatcc
    16261 atggtgatga ctgatcggcc cgttgcggct tccaagcccg cgcacagcgc cgcctgatgg
    16321 ccgaagtttc gcgacaggaa cacgcctttg acggtcgagt cctgcacgtg aagatcgcga
    16381 atgacctccg ccgtgcgatc gcgactgccg tcgtcgacgt aaatgatctc gtagggaatc
    16441 gccagacgtc gcatgaccgc gctcagacgc tgatgaagcg tcggcaagct ctgctcttcg
    16501 ttgcacaggg gaatgacgat cgagcactca agagccaccg gggcactcct tcttttgata
    16561 gccggctgat cctcgctcag ggggccataa gccctggttc ttaagcatcg gcgggtttcc
    16621 ggaccgcgtt aagcttaatc catgcagcag aagggattat cagacgttgc acaagcgatg
    16681 aaacggcgac agggcgagca acagttgcgt ctgcaggcaa ccgatattat gggacggcat
    16741 ccggacgtag ttcagcaggc aacggcgaca acatgatcac agcaacacgg tacatgcatc
    16801 ttcgatccag cgacatggcg ccgcccttgg cgggcattcg agccggtacg ttcgctgctg
    16861 taaccgtatt catcatcgtg tgggtgatct cgctcagccg gagcgttttc gacccgatcg
    16921 gattcgacca ggcgctgtat cagtacatca ccgatcgagt gatgctcggc cagcggcttt
    16981 atgccgacgt ctgggaccag aacacgcccg gcatcatcgg gattcattgg ctggcgacga
    17041 ttctcgtcgg ccgaacgccg attgcactgc gggttttcga tgccttctgg caatgtgcga
    17101 cgattaccgc gttggtggct ctggcggtcc gcgacgggcg gcgctggcaa gccggctggc
    17161 tggcggcgac tttgtatgtc ctggcgtact atgggctggg ttacgtgcat accgcccaac
    17221 gggaaggctt cgcggtgctt ccgctgctcc tggccgttca tgccctcgcc cctgcgacgg
    17281 gacatgaccg cgcgtctcgt gctcccgctc ctcgacatgt tctggcaggg gcgctctgtt
    17341 tctgcgtttt cgccatcaag ccaccgctcg gcctttgttt cggaatgctc tggctcatga
    17401 ttctggttcg ttggcttgcc ccgctcctgc gtccggctgc accctccctt cagaacggtg
    17461 cttcaccgct ccagcaactc tgccccggcg cagccttagg cgaaacgaga catgagagca
    17521 gattctctat cacacgtttg tcagaatccg ctgagaaaag agccttgttc tcgctgagcc
    17581 tgggattcat cgtctcggcg gccctggcgg tcgcgcttgt gcatcagctc ggctggtgga
    17641 gcggcctttg gcgaacgctg gcccgtcagg acgtggcggg ttacatccgt gggccgcaac
    17701 tgatccgcga atcagctcct taccttttca ccggtgcagt cggcattgcg ggcatcgcgc
    17761 tgctcgcaca cgtacttcga gccaagagtc atgcgggctc aagcctgcgt ctccgtgacc
    17821 ttgccgcacc ggttacaggc ggcttggccg ttttcgcgtt gctgttgact attcagcgat
    17881 ggccggactg gcaacaggcg ttccttaaga cggccgggat cctcgtgccg gccggcgggg
    17941 ccatgcttct ggcccaatgg ggccggaggt cgcggatatg gcagatgacc gctcttttgg
    18001 gccttgcatc gctggccgcg gtcttgtggc aaggccactt tgcgctttat cagttccctc
    18061 cgttgttggc gtgcggggca tatatcgcgg cggcggagat ttcggagcgg ttcccccgct
    18121 tctgcgagat ggacaaggcc gggcgcgtat ggaccgtggt ctgcctcggt gcggtgatgc
    18181 atttggccgc cggcacctgg ggatggacga tgaccttcta cggccgttct ccgtacgttt
    18241 tgagcggcac gacgctgatg cagcattaca ccaaggtcac cgcgaacaaa cccagcttcc
    18301 cgacttacga gacaacctcc gcggcagcag ttcgtgtgcg ggagctgacc ggcgagaccg
    18361 acccgatcgt ttgcctagtc gatgagccgc ggctttacta cctggctcag cggccgccgg
    18421 cgtatccgct gctgcgtacc caggtctgct acagcagcat gtttcccgcc ttgtttgagg
    18481 ccatccgaac acaacacccc aaggtgttgc tggcccgtct gccggtcggc gcgcgaggcg
    18541 ccggtgatgc cagggcagcc gaagccgccg tcctggccga cttggagggc tacttcggcc
    18601 cgccggctcg agttgtccgc gacgaataca ggctgacgga ggtcgtcaac ggcgacctgt
    18661 gcattctgca gccgcaagct cgtatcagta gtaacgcgga tcgttgacca cgccttcatc
    18721 actgatcaga acggacagca ccgggtcctt gagcacgtct tcaacaagca tgggccggcc
    18781 gtcaacgagc atcacccgct tgaccagtcg aattccatga ctgtagtcca tgtaggtgat
    18841 cgtgtgtccc agatacagag gctggatggg ctgaccgttg agctggtgcc atccgtagat
    18901 ggccaccttg ccgggatgcg tggccagttg cgcggtgaca accacgtcct tcttggtgcc
    18961 agcaatgagc gtgcccagcg atgtcccggc ggcgatccgc tggccttcaa tgtaggtgtt
    19021 gctcatgttg aacacctcga ccgactcgat gttgtacacc gaagggctgt aggggtgcgg
    19081 ggccagcttg cacggtgcgg ccgaccagat gtcgttgacc atcttgcggg tcggcatcat
    19141 gcactggaac gtatcgccaa tggattgagc ggtcagcggt cccatcggga agcggacgaa
    19201 atctgcgtct gatccgatcg ccagataatc cggcatcacg aaccaggtcg ccgtgtgctc
    19261 aacggaggcg atcgtcgccg tgacagtgac cggcacgaaa cgccttagaa actcggggat
    19321 gtttccggcg gtcatctcag ccaggatcat ctgctcgcgc gtggccttgg gaatgctcca
    19381 gacctgctgt acgaactggc tgccggtgac cgctccggca ggccgaggcg ggatggggct
    19441 ggacaggcat ccagaggtgg ccggaacgtc cgggccgctc agacaatgct ggagcacccc
    19501 gaagtccgct tgatccacat cgagatcgcc gtcgaaatcg gcccagtggc aggcatcggc
    19561 taccggccct gcgtccgtgc cggtgatgca cgccaagaag atcgccaggt cgctgccgtc
    19621 cacgtcctga tctccgtcga aatcggtgtt gaccggcatg tcaccggcca gcacaaaccg
    19681 gactgcatcg gcaatgacga cgtcgccggt ggccacgttg ttgaggcgca catagccgcc
    19741 tgtgccggcg gcaaaagaat aggaacccaa cgagaaccat tggctcccgt ttgtctgctg
    19801 gttgacgaat acggtgaccg ggtcgccggt accgtggatg acaaatggcg cgttcgtcgc
    19861 ccggttggca ccttcgacgt accagacgaa gacctcatag gtgcccgacg cggggagacc
    19921 aggacgccat tcgacctctc cagtcgggct tcccgcgaca gtggcccgcc atcgatagtc
    19981 ggttccatag ggcgtcggcg aggtgccggt catccatgtt ccgctgagaa tcgtgaaacc
    20041 cgagtcgctg ttgtcgacga caatctcgac ggctccaaca atacgggacg acgccaacag
    20101 cagcgacacc agtaggattg ccatatgata atcactcgac tttgcagcca tcgggttttc
    20161 tccatgttga cgcattttaa caagtacagg ccccggccca aaggcatttg ccactcttgg
    20221 aaccggcgag cggcgcgcga ccgtctcggg ccactctcag aaccccaagc acaccggtcc
    20281 gccgtagccc tggcgaaggt ggaagcgagg ggccccggcg acctgccgag ggtatcgcct
    20341 tttttgaccg gatccgcttc gatggaatgg aaatctacaa cacgcacagc gacgtgaaag
    20401 atgagtccgt gaagatgctg ctcgcagcga tgatcctcaa tctcccggcg gtgagggcgc
    20461 attcggaggc tgctttcctt tcctttttcg actacccggc tgaattcttg gcgagattcg
    20521 atatcctgag gctcgaaagc ccgtttgccg ggatcgtcgc gaatgacagc caccagaacc
    20581 agggcttcaa ggtcatggcc atgcccgacg gagggctgga agtcttcgac gccgccgagg
    20641 aatcggtctt caggagcgac ggcgtcgtgg cccggatgct tctcgcggcc ctgggctgca
    20701 ccaagacgcc cgaacagacc ctccagttga gccgtatcca gcttgcccct tacgagatca
    20761 gcgtgcggca cgtcggtacg ttcctccaga tcgaccacat cgacgagaaa acggtccggc
    20821 acgccctgcg gaccggccgc attgtcctcg gcttcgagat catcgcctca cctgccggcc
    20881 gtcggtttct gggtggagcg ggccggcaca ccggccggaa cagtcggcga tcggatcccg
    20941 tggcagccga agccggcgct ccggtgggcc cttcctctgg acgccgaggt ccggaacctc
    21001 cgcaacgggc gtccatttca tgaggttgcg gcccaatccg gtacagtcga cgacatcctt
    21061 gcgggagtgt atcgtctgga agcgtgcctc accctggtcg gcgagcgccg gccgtgggtg
    21121 attaccaatc ccgtctacgt aaggccggtg caggtcgagg agaacgccac cggcaagtag
    21181 cagtcggcaa ccgtgggaac gtttcttaag gagacctgac gatgagaaag tccatgtgtg
    21241 caatagtgat cgttctgccg accgtcctgt gcgcgctcgc acgcgcggat ctggccggtt
    21301 acatcgacaa aaaagacgcc acctattcct ggaccaccgt atcgacgagc gagagcgatc
    21361 cggagagtcc gatccggtac gctgagcttc gaatggtgtc ccagacctgg cgggggatcg
    21421 aatggaagca tcggatggcc gtcgtgtgcc cgctcaagcc gcagacgacc tcgcacgccc
    21481 tgttgatcat ctccggcggc tcctggcgcg agggccagga gcagccgaag ctggacaagg
    21541 actccaggga gttcaagctg gccacaacca ttgccggcgc ctgcggcatg ccggtcgctt
    21601 tcgtgatgca cgtcccgttt caacccatgt ttgggggctt gaaagaggac cagatcattt
    21661 ccaagacctt cgacgagtac ctcaaaacgg gcgatgagga atggcctctt ctgctgccca
    21721 tgaccaaggc cgccgtgcgg gccatggaca cggcccagga cttcgcaaag aaggaatgga
    21781 ggatcgacat ttcgaagttc atcgtgacgg gggcctccaa gcggggctgg accacctggc
    21841 tgacgggtgc ctccgacaaa cgggtcgtcg ccatcgcccc gatggtcatc gacgtgctca
    21901 acatggcaga gcagatgaag caccagaagg agacgtacgg cacatacagc gaggagatcg
    21961 acgattacac caggct
//
LOCUS       DAOTPW010000079        21822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2468_length_21822_cov_5.536454, whole genome shotgun sequence.
ACCESSION   DAOTPW010000079 DAOTPW010000000
VERSION     DAOTPW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21822
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2468_length_21822_cov_5.536454"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..2963
                     /gene="ppdK"
                     /locus_tag="P5316_09025"
     CDS             192..2963
                     /gene="ppdK"
                     /locus_tag="P5316_09025"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRR85140.1"
                     /translation="MAKKWVYLFGNGKAEGDGSQKDLLGGKGANLAEMALLGLPVPAG
                     FTITTEMCSEYYRRGKKFPPELKKQVEHALVQVEKAMGKKFGDPKNPLLLSCRSGARQ
                     SMPGMMETVLNIGLTSKTIPGMIAKTNNPRFVWDSYRRLMQMYADVVMEKAEGIEPPE
                     DMGVRRQMERAMEQLKRSKGYKLDTDLTVEDLQALCDTYKKIIQDVLGKSFPDDPMEQ
                     LWGGIRAVFQSWNGKRAISYRRIENIPDDWGTAVNVQAMVFGNMGENSATGVAFTRNP
                     ATGEDAFYGEWLVNAQGEDVVAGIRTPSPLNEATRPEGNTEPSLEKANPKTYKQLHAI
                     RDKLEKHFREMQDIEFTIEEGKVYMLQTRTGKRTGPAAVRIAVDMAKSKLITPEEAVM
                     RVTPAQLDEVLHDVLDPLAEKSAIKLCTGLPAGPGGATGQVVFTAEAAVEAAGKGKQV
                     ILVREETNPEDVEGMRAAVGILTARGGMTSHAALVARGWGKCCIVGAGDVHVDLAGRS
                     LRVGNEIIREGEWLSLNGTKGVVYKDRLPMVAGAEDNPYFKQFMAMCDKIRKLGVRAN
                     ADTPADAAKARKFGAAGIGLFRTEHMFYGKGAEEPLFKLRKMIHARSLEERKAALDEL
                     FPHVKSDIKATLEAMDGLPVTIRLLDPPLHEFVPQKEDERRKLAAGLGISLEELEKRA
                     ESLDEVNPMMGHRGVRLGVTYPEVSEMQVRAILEAAAELIKAGKKAMPEIMIPVTCDV
                     SELKHQKQIVDRVAGEVAKKFGMKKISFLYGTMIEIPRAALKADEMAQVAEFFSFGTN
                     DLTQMTFGFSRDDVGGFVPEYIKGKLLPADPFQILDQSGVGELVKLGIERGRATRPGL
                     KVGICGEHGGEPESVKFCHRVGMDYVSCSPYRVPIARLAAAKAVVEEKRTQSAKPAKK
                     GKKTPKRKK"
     gene            complement(3309..3632)
                     /locus_tag="P5316_09030"
     CDS             complement(3309..3632)
                     /locus_tag="P5316_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRR85141.1"
                     /translation="MGKKLYVGNLSYSTTSDELAELFAPHGSVQSAEVIMDRASGRSK
                     GFGFVEMGTNEEAQAAISALDGQEVSGRPLKVNEAKPREDRGGGRGRGGHGGGGGYGG
                     GRGRY"
     gene            4460..5377
                     /locus_tag="P5316_09035"
     CDS             4460..5377
                     /locus_tag="P5316_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85142.1"
                     /translation="MPRIAGPWWTVARSPDLGGLNGPPAENPNHRQEPVDFAIWQAAD
                     GTWQIWSCIRNTRCGGLTRLFYRWEGKRLTDPDWTPLGIAMQADPKYESRVGGLQAPY
                     VIRIGDLYYMIYGDWDWLMIQRSRDGKVFERWPYPNGKLGMFTEGPEANTRDPMLIRI
                     NDLWHCYYTAFPNQIGAVFCRTSKDLRTWSESKVVARGGLTGTSPYSCECPHVVKIGD
                     WFYLFRTQRYAGPPTTSVYRSKDPMDFGIDEKADDKFVCLLEVAAPEIVFHGNLYYIA
                     ALLPDIQGIRIARLTWIPDSMNRGGSTTN"
     gene            5441..6550
                     /locus_tag="P5316_09040"
     CDS             5441..6550
                     /locus_tag="P5316_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR85143.1"
                     /translation="MPSFNGLGMHLGNLPRLSNAKTRSISPENFTGEKGKGGMATEGL
                     GREAARQLGQGWKISPAIKIAAGEERELANIQGPGAIQQIWMTPTGPWRMQILRIYWD
                     DQAMPSVECPIGDFFACGWGRYAHVNSLAVCVNPGSAFNCYWEMPFRKKCRITFTNLD
                     DKEMVLFYQINYTLTEVPEDAAYFHAQFRRCNPLPYKTDYTIVDGIKGKGQFVGTYMA
                     WGVNNNGWWGEGEIKFFIDGDTQWPTICGTGTEDYFCGSYCFIVNKGPGGTREGKGEQ
                     YQEYSTPYAGMPQVIRPDGVYDANTRFGLYRWHIMDPVRFEKDLRITIQALGWRSHGR
                     YLPLQDDIASVAYWYQTLPTAPFPKLPDRDHLEII"
     gene            complement(6721..8319)
                     /locus_tag="P5316_09045"
     CDS             complement(6721..8319)
                     /locus_tag="P5316_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRR85144.1"
                     /translation="MEFALNSFDLSIIVTSLAAVVAVGFWASRRQDKTARGYFLASGR
                     LPWWIIGSAFVSTSVSSEQIVGTVGAAYEHGMAIANWEWCSLPVYTLLMIFFIPVYLK
                     NRVTTVSEFLHRRYGTLCSDFYSFVMLFAYVFIFLVPVLYGGTLAISGLTGWPFHLTL
                     WATVLIVAAYTVKGGLASVMWTDAMQCLMLVGGGVLLFFAALGRVDGGWNAMIAASPD
                     RFHLYRPPGDEAAPFLGLITAMFGVILFYQAGNQVMIQRVLGARSEWDGYMGIIFAGF
                     INFIRPLVTCFIGLIVYHWIHVMNMADPLRDRDLAFPFALRMLAPDWGLRGIVLAGFL
                     AAVMSTLSALSNSTATLFALDVYRKWLRPHADDVQTVRAGRIASLLALVIAALVAPAV
                     AHFGGIFKYFQQGVTYLSTPFLTALFLGILWKRANYQGGLFALFGGTAIQAVIIAGFH
                     LAGVKLHWLYLAFFAQVLTIAGAVIVSLRYPPPPAERWEPFNWSPALLARRADAPPRP
                     WYKSLILWFSIYAAIWFYLYRRYW"
     gene            8830..9990
                     /locus_tag="P5316_09050"
     CDS             8830..9990
                     /locus_tag="P5316_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85145.1"
                     /translation="MKAVNLSSDDSEWRLGGLNGYAVILAVVHLVVACPILVLVAWAA
                     NNGGMTGKCVPLLAFAVGPFVVFAVYSGLFRLIAGRWTLVPNFAFLACVAGVWFVGAT
                     KTCQTRMISVAQAEQTHQNLSSQVEGLTESLRAFVEKTGQYRMEAKPLANYLKAAEAA
                     GLPRPAVEECCNTAMTLLKEERHKRAVRHDAISRLNAAGGTQAKTITSRKDAESRLKL
                     AQQILKAAEDVQQHMTSGSERIRDILVKAGINGTRLEQLRGCVPGYGVSAEDKSACTA
                     MLEYDQTRVAYFSLLADKWGQWQWDRDRFASSDPAIVKEYKRLSDVLGKTEANADLPP
                     RQTQGVSSVNKTQNPQPVKNAQNANPARKSKTATPAKKAASASSKDKRQTIR"
     gene            complement(10073..12163)
                     /locus_tag="P5316_09055"
     CDS             complement(10073..12163)
                     /locus_tag="P5316_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85146.1"
                     /translation="MARRWPTTALIAAFLTVTGGSIAQTASRPATAVVEILPQISETP
                     VWPEIIGVNLDYGGAAALKRPEAMQAVKKIGIKSIRFPNGCEADRYDWKADNERKMTV
                     EQFLVFCEAVGAEPYYTLNLQGGTEGLPGPPPTGAPVAEIIRYRHTAPNPCGYTDYYF
                     GTLAETLNLVRKYTIERALAGRLPITCYEMGNENWGQATTDWPPEHYAPTVAAYAQAM
                     RQLMAEAAAQHPTIKGLKLWITAVGYPLMGNNQDPLQATNHDINVRWTRELNQLYEAG
                     LIDAVQDHFYPYSIEGTDLLIWSHHNLQNILYGRRGVANPGLGGYREEAIAYRSPIEI
                     TEWNLKCWGEQRKSKLDAKNLDFEQDMTGWRVTHGVAGSTAVVSQDAGRRGRGLRLRT
                     GSSADASISVSQAFEWKDKKATSIRVAAWVRTPHPDRISAQLTTLGPESRPAEPLSDS
                     ASRTAWRANHWHRIVFGGKVPEGATSLAVNFLLSGADAEADIDGVELYYWDNEAGLMP
                     AAADTAAQQLLLVDAFRVMISHGIRRAHLHHLFGAYPCGIMYTDGRIKDNYKVFEFYA
                     GGLGTHTVRTQVTCDTFDFDSQGDKYATAFNALAPDVKNVPIISALAMRDESSLYVVA
                     VNRSTDEPVDLALRVRDADLGAGFVRTLTCQDTDRPGVQLGTQDLPSGPPATYRMAAH
                     SACLFKLAMTSQKR"
     gene            complement(12335..13102)
                     /locus_tag="P5316_09060"
     CDS             complement(12335..13102)
                     /locus_tag="P5316_09060"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRR85147.1"
                     /translation="MPWDKLPYLCVLAVLVAASFGMPIPEDIPLLTGGWLCYRGLAVL
                     PIMIAVGMLGVLTGDVCLFFMGRRFGHHIVEHRFMRRIVKPSRLLLAEHLFEHHGIKI
                     IFAARFLPGLRAMVFLASGVMKVPFWKFISVNGSAACISVPTLVVLGWLFGSQFDQLK
                     SDVRMVTNIIGFLGLIVLVVAVGLYFHRRSKQYLAEAGPDEAVDADTLVQLPPGGHVE
                     HTSAVGKPAAPMSASTVSEQVSSSHNDSSAAQVAGGV"
     gene            13349..13819
                     /locus_tag="P5316_09065"
     CDS             13349..13819
                     /locus_tag="P5316_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRR85148.1"
                     /translation="MGNTHNKWYAEGLRFSCSQCGNCCTGPPGYVWVTREEIRRISVY
                     LGREDDWLGKEHLRRIGFRHSLVEKPNGDCVFLMSTGNGTRGCSIYPVRPLQCRTWPF
                     WTQNLKSPSAWAAAGEVCPGMNNGRQFTIDQIEAVRLKDSWEPGEESPLKADAP"
     gene            13838..14293
                     /locus_tag="P5316_09070"
     CDS             13838..14293
                     /locus_tag="P5316_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85149.1"
                     /translation="MSERLLPVSVSVLREHAQRPEIVRAMREFYVEVDREIAAHDPTC
                     WNKGKCCCFGQFGHRLYVTSLEIAYYLATGNLTPPVTSDACPHAIDGKCHARERRPFG
                     CRVFYCDPSAQHWQGPLSERRLTQLKAMHEALQVPYMYVDWMTVMKGMQ"
     gene            complement(14406..15257)
                     /locus_tag="P5316_09075"
     CDS             complement(14406..15257)
                     /locus_tag="P5316_09075"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR85150.1"
                     /translation="MAVSSNSTISLSHADRIAPWESLLQTTAQSVSLVIPAYNEELCI
                     GRSLEEVMAYVEAWPAFREVIVVDDGSTDRTAVIVEGSRRQHAATPVQLILLRHQRNQ
                     GKGAAVRTGMFHATGDIVAFTDADLSSPMSELPALVGPILAGQCDIVVGSRALDRSLI
                     AMRQSRFRETAGKIFNLLVRMLTGLPIYDTQCGFKAFRRSKIDPILRLQRVETFAFDV
                     EMLYLASRMGLTICEIPVHWSHVSHTKVKLFRDSIRMFLDVLWVRFDAFRGRYHLTDQ
                     QTNRPTD"
     gene            complement(15257..17953)
                     /locus_tag="P5316_09080"
     CDS             complement(15257..17953)
                     /locus_tag="P5316_09080"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRR85151.1"
                     /translation="MTFPRFAIFLGMVLAVWFWPSIFGGKVLLPTDLTWQYPPQTPPA
                     GVTGVHNSLIGDMLYENYAWKTLLRRCLADGEWPLWNPYAFCGHPLYATGQASTFYPL
                     NIIFVLAPLPQAYVIYTVLHLWLAGMFQYLFLRRIGVSPFGCAVGGVAFATCGFLAMQ
                     LIWPMLIGSAIWLPLMLLWILRLGDCFRAVGDSLPRAGANGIPESMAGSGTRQSSAKM
                     VPSPFRRAAGWRIGLGGVIFAMPILAGFFEIAFYACFTAALFTLACCWHLWRQTRSAK
                     RCLGFCAATVTTAALAAMLAGPQLLPFLEVKNLTTRAAEASYERMVNRALRPEHLLEM
                     IVPDIFGNPAKHKAFDLRTRSMRPIEARRGADFYAYGTKNYNENGFYLGLMPLGLMLI
                     GLLAHGCYRWFFILVLALSLLLAFGTPIYAVFYYTVPGFEQVRTPFRWMFAGTFAICC
                     LAAMGGQRWFGRLSGSWCDEMRESLKKCRDGSLDAAVPVASQASGQRRITGVLIIAVP
                     TLMLLALLLMLVFPSLAYRLAERAFDQIPRLSQGNGFIDSWDVAGYMWANALRLTIFA
                     LAAAVVLALPVLRVWKPRGAAVAAMLCLLLTAADPFQANGGFMTQADPAWLDHVPPAV
                     EFLRSDSGPFRIGRFGPRMVLHPNLPMLYGLQDIGGYDSVILADYADYINAIEPQRAL
                     WSNQIIGFEDPGSLDSPLLSLLNVRYLLVDPSDELDHPDWELVFREGLNIYRNKRECP
                     RAFMVYEVRETASCEQAVADLAEGHVDTSRIALVEAAASAIGLPSAAPGEPGRVRIVR
                     YGHSAVTMTTSTSVPGLVVLCDMMYPGWRAYVNGRSADILKVNGIFRGVHVPAGECRV
                     EFRFEPGSLRAGLVLMCLGLLAVGGCFAASCRPSRRKGERLA"
     gene            complement(18080..18769)
                     /locus_tag="P5316_09085"
     CDS             complement(18080..18769)
                     /locus_tag="P5316_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRR85152.1"
                     /translation="MSDPVILDNIAFDLEPEAVLDRLHLRDLDDYHSRALDLAGQAQV
                     VARPRAMYRVAYIDSRTDDTVTVEGITFTSRVLAVNLRETHRVFPFVATCGREMAAWA
                     APITDMLERFWAETLMEMAVHAAARFVHKHVTDKAAFGPVAAMDPGSLQDWPLEQQRE
                     LFTLLGDTCGAIGVELTDSCLMVPVKSVSGIFFPSETHYENCQLCPRETCPGRRAKYD
                     PDLYARRYAQH"
     gene            complement(19201..20211)
                     /locus_tag="P5316_09090"
     CDS             complement(19201..20211)
                     /locus_tag="P5316_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR85153.1"
                     /translation="MNSRERVLSMIEGRALDCLPLMPITMMFAADQIGAKYREYATDY
                     RVLAEGQIRTAEQYDFDYVSVISDPARETADCGGTIEIFDDQPPAIVESEARLTDKSD
                     LASLKMPDPLGGGRMTDRVKGVALLRERAGKDKIVEGWIEGPIAMGADLRGLNALMLD
                     LIDDPAWVRDLFEFVVEMELCFAKAQVDAGVDIIGVGDAAASLVGPELYNELVWPYEK
                     KLVDGLHAMGTRVRLHICGNTRPLLTDMGRLKCEIVDLDSMAPLDEARAAMGPDQVLL
                     GNINPVAVLRNGTPEQVHAALAECHRQAGARYIVGAGCEVPRGTPNENVLAMRDYARA
                     NH"
     gene            complement(20536..21774)
                     /locus_tag="P5316_09095"
     CDS             complement(20536..21774)
                     /locus_tag="P5316_09095"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRR85154.1"
                     /translation="MTDKTYRPKRWIDMRKLGWFSGDDHVHGQILSDDDAARLMAWLV
                     AEDVHLGNIVKMGDITRTYFEQRGFGKDYRVINSDYVLSPGQECPRTHQFIGHTISMN
                     INSMVRDTNKYYLYDYVADTVHGQGGLWGYAHVNSGMFHVHRDMSVNVPKGKCDFAEV
                     LQFGHLGTELWYQFLDLGFKVTASCGSDVPWGGTIGEVRLYACIGEKAFDPDAWFEAV
                     RRGRTFVTNGPMIEFTVNDALPGDEIVVKDNAKLHVRVKAMGQTEFSQPESVKVVKFG
                     QEVRVAKPAKEGDTEIVLEFDMEAGHGCWLAAVTRATNGCHAHTTPIYVRREGLRWWK
                     FEDVDQLLDKRLDSLSQIEKLVADAKSKDLVTLEGDQELKQLALQGDELLKRVDAARK
                     IYEQLRETAVKEKAIRGSNK"
BASE COUNT         4554 a         6588 c         6770 g         3910 t
ORIGIN      
        1 aggcgagtcc gtccgtactc ttcttggtcc tttgtgctcc cgagccggcc gtgttggagg
       61 gtcgggcggg ccagccacca gccgtcggag cagacgcccg ttcgggtccg aggccggtga
      121 cagcaaaccg cagacgtgct tgcgcacacg tataccataa caggggcaaa ccccaggaga
      181 aagcttacac catggcaaag aagtgggtct acttgttcgg caacggcaag gcggagggtg
      241 atggcagcca gaaggatctg ctcggcggaa aaggagcaaa cctggccgag atggcgctgc
      301 tcgggcttcc ggtcccggcc ggtttcacga tcaccacgga aatgtgcagc gagtactatc
      361 gccgcggcaa aaagttcccg cccgagctca agaaacaggt tgagcatgcc ctggtccagg
      421 tcgagaaggc catgggcaag aagttcggcg atccgaagaa cccgctgctg ttgtcgtgcc
      481 gttcgggcgc acgccagtcc atgccgggca tgatggagac ggtcctcaac atcggcctga
      541 cgagcaagac gatcccgggc atgatcgcca agacgaacaa cccccggttc gtctgggact
      601 cttatcgccg cctgatgcag atgtatgcgg acgtggtgat ggaaaaggcc gagggaatcg
      661 agccgcccga agacatgggc gtccgccgac agatggaacg ggcaatggaa caactcaaac
      721 gcagcaaggg ctacaagctg gacaccgatc tgaccgtcga ggatctacaa gccctgtgcg
      781 acacgtacaa gaagatcatt caggacgttc tgggcaagtc gttcccggat gaccccatgg
      841 agcagctttg gggcggcatc cgcgcggtct ttcagtcatg gaacggtaag cgcgcgatct
      901 cctacaggcg catcgagaac attccggatg actggggcac ggcggtcaac gtccaggcaa
      961 tggtgttcgg caacatgggc gagaacagcg ccacgggcgt cgcatttacc cgtaacccgg
     1021 ccaccggtga agacgccttc tacggcgagt ggctggtcaa cgcccagggc gaggacgtcg
     1081 tggccggcat ccgaacccca agtccgttaa atgaggcaac acgccccgaa ggtaacaccg
     1141 agcccagcct cgaaaaggct aatcccaaga cctacaagca gcttcacgcc atccgcgaca
     1201 agctggagaa gcatttccgc gaaatgcagg atatcgagtt caccatcgag gaaggcaagg
     1261 tctacatgct gcagacccgc accggcaagc gcacggggcc ggcggcggtg cgaatcgcgg
     1321 tggacatggc caagtcgaag ctgatcacgc cggaagaggc ggtaatgcga gtgactccgg
     1381 cccagctcga tgaggtgctc catgatgtgc tcgacccgct cgcggaaaag agcgcgatca
     1441 aactgtgcac cggccttccg gccggcccgg gcggcgcgac cggacaggtg gtattcacgg
     1501 ccgaggcggc cgtcgaggcg gccggcaagg gcaagcaagt gatcctggtc cgtgaggaga
     1561 cgaaccccga ggacgtcgag ggcatgcgtg cggcggtcgg cattctgacc gcccgcggcg
     1621 gcatgaccag ccacgccgca ctggtggccc gcggctgggg caagtgttgc atcgtcgggg
     1681 cgggcgacgt acatgtcgac ctcgccggca gaagcctgcg ggtcggcaac gaaatcattc
     1741 gcgaaggcga atggttgagc ctcaacggca ccaagggcgt ggtctacaag gatcgcctcc
     1801 caatggtcgc aggggccgaa gataatccct atttcaaaca gttcatggcg atgtgcgaca
     1861 agatccgcaa gctcggcgtg cgcgcgaacg cggatacgcc cgcggatgcc gccaaggccc
     1921 gcaagttcgg tgccgccggc atcggactct tccgcacgga gcacatgttc tacggcaagg
     1981 gagccgagga gccgctgttc aagctgcgaa agatgatcca tgccaggtcg ctcgaggaac
     2041 gcaaggcggc gctggatgaa ctcttcccgc acgtgaagtc ggatatcaag gcgacgcttg
     2101 aagcgatgga cggcctcccg gtgaccatcc gcctgctgga tccgccgctg cacgagtttg
     2161 tgccccagaa ggaagatgag cgaaggaagc tggccgccgg tctgggcatc tcgctcgagg
     2221 aactcgagaa acgggctgag tcgctggacg aagtgaatcc gatgatggga caccgcggcg
     2281 ttcgcctcgg tgtcacctac ccggaagtca gcgagatgca ggttcgggcg atcctcgagg
     2341 cggcagccga gctgatcaag gccggcaaga aggccatgcc cgagatcatg atcccggtaa
     2401 cgtgcgatgt gagcgagctg aagcatcaga agcagatcgt cgatcgcgtg gctggcgagg
     2461 tggcgaagaa attcggaatg aagaagattt cgttcctgta cggcacgatg atcgaaatcc
     2521 cacgcgcggc gttgaaggcc gacgaaatgg cacaggtggc cgagttcttc agcttcggca
     2581 cgaacgatct gacgcagatg acgttcggat tcagccgcga tgacgtggga gggtttgtgc
     2641 ccgaatacat caagggcaag ctcctgcccg ccgacccctt ccagatactg gatcagagcg
     2701 gggtgggcga gttggtcaag cttggaatcg agcgcggccg ggcgacccgc ccaggtttga
     2761 aggtcgggat ttgcggcgaa cacggcggcg agccggagtc ggtcaagttc tgccaccgtg
     2821 tggggatgga ctatgtgagc tgctcaccct accgcgtccc gatcgcccga ctggcggcgg
     2881 caaaggcggt ggtcgaagag aagagaaccc aatcggccaa gcctgccaag aagggcaaaa
     2941 agacaccgaa acgcaagaag tagaacgcgc cgcttcggtg ctggcgatca gttgctgact
     3001 ggacgattga caacagaacg ccccgcttct cctttcggag aggcggggtg cccttttttc
     3061 cacaacctcg gcatcaccgc gataccgttc gatcgcagac acctgaactt gtacttgctg
     3121 gcggaccctt gtcgggtatg tgattgcagc ctatccagga acgaccgtct ggtccttgtt
     3181 caacaggaaa gccacgaaca caccgaggta tgctcgtggc cttcggctac tctagggttc
     3241 gcagagtatt gcgaaagccg acatcacgat cggccggaag agccagatcg ctgtcggctt
     3301 caggacattc agtagcggcc cctgccacca ccatagccgc caccgccgcc atggccgcct
     3361 cggccgcgac cgccaccacg atcttcgcgc ggcttggctt cattgacctt cagcggcctt
     3421 ccgctgacct cttggccgtc aagggccgag atcgcggctt gggcctcttc gtttgtaccc
     3481 atctccacga aaccgaagcc cttgctccgg ccactggccc gatccatgat cacttccgca
     3541 ctctggacgc tgccatgagg tgcgaaaagc tcagccagtt catcgctggt ggtagagtac
     3601 gacagatttc cgacatacaa cttcttgccc atcgtcaatt ctcctgttcg agggcatcac
     3661 gacccgcatt tctctttccg ggtcctttca gaaagtggcc aacattccgg ccaagaaggt
     3721 aggagggcga ttgagattgc agtgcggact tgttcaacca acgtcacgtt cctgactcgt
     3781 tcgacaaacc aaccagaaaa cactatacac tgcaattcac gaaaagtaca gcccgcgttc
     3841 tccgaaatgt gcctgcccac cttcttcaga ccgcaagtct gggccggaac gtgggaggcc
     3901 gccggcaggc ctggacaggg cgcggggccc tctccaaccg gcgaaaacct tctaaactgt
     3961 tgatatggct aatgatacga ttgtttcggc ccacggtacg tgtggcgacg ttacccattt
     4021 ccgcaagtga ctgcttaccg acaccccgcc gcgagttccg gttcgcgatt ggcccgttgc
     4081 cgattgcctt ggggccgggt cgcttgacat cttttttgcc ttctcgacta tgtgtcgtgg
     4141 gccaggaacc ttgatcggat cgtttgccgg gccctacgga tgagtcgttc attggaacct
     4201 tgcggccgga catctgtgcg ccttgcgcgc atgtttccgg atcgccgggc gccatgccca
     4261 cggctctgcg tgggcatgcg cctttagtct cctccgtcgc gacgggtgtg cgctttggcg
     4321 catcttttgg ttgcggccga aggccgcctt gggaagacag tcttcaatgt tgagaaaccc
     4381 caccgcagca ctctgtaccg taacgttctg ggcgaccctt ctttgcttcg gctgcaacgg
     4441 cacggggcaa agcgcgctca tgccacgcat cgccggtccc tggtggaccg ttgcccgcag
     4501 ccccgacctg ggcggattga acgggcctcc tgccgagaac ccgaaccatc gccaggaacc
     4561 cgtcgatttc gcgatctggc aggcggccga cggaacgtgg cagatctggt cctgcatccg
     4621 caacacgaga tgcggtggtc tgacacgatt gttctatcgc tgggaaggca aacgcctgac
     4681 cgatccggac tggacgccgc tggggatcgc catgcaggcc gatccgaaat acgaatcccg
     4741 cgtcggcggt ttgcaggccc cgtatgtcat tcgcatcggt gacctctact acatgatcta
     4801 cggggactgg gactggttga tgatccagcg gagccgagac ggaaaagtgt ttgagaggtg
     4861 gccctatccc aacggcaagc tgggcatgtt taccgaaggc cccgaagcca acacccgcga
     4921 cccgatgctg atccggatca acgatctctg gcattgctac tacacggcct tcccaaacca
     4981 gatcggggcg gtgttctgtc ggacatcaaa ggatctgcgc acttggagcg aatcgaaggt
     5041 ggttgctcgc ggcggcctca cgggcacttc gccatactcc tgcgaatgcc cgcatgtggt
     5101 gaaaatcggc gactggttct acttgttccg tacacagcgt tacgccggtc cgccaaccac
     5161 cagcgtttat cgttcgaaag atcccatgga tttcggcatc gatgagaagg cggatgacaa
     5221 gttcgtctgc ctgctggaag tggccgcccc ggagattgtc tttcacggca acctctacta
     5281 tattgccgcc ctgctgccgg acatccaggg catccgcatc gctcgcctga catggatccc
     5341 ggacagcatg aaccgcgggg gatcgaccac aaactgacga cggaggccaa cggcaggatg
     5401 ccaatcgctg atagccctcc gtctcttcca ggagactacc atgccttcat tcaacggact
     5461 cggtatgcac ctcggtaatc ttccacgact ctccaatgcc aagacccgat cgatcagccc
     5521 ggagaatttc accggcgaaa agggcaaggg cggcatggcc acggaggggc tgggcaggga
     5581 ggccgctcgc cagctggggc aaggctggaa gatctcgccg gcaatcaaga tcgcggccgg
     5641 ggaggaacga gaattggcca atatccaagg ccccggcgcg atccagcaga tctggatgac
     5701 tccgaccgga ccgtggcgaa tgcagattct ccggatctac tgggacgacc aggcgatgcc
     5761 gtcggtcgag tgcccgatcg gcgacttctt cgcttgcggc tggggccggt acgcccacgt
     5821 caattcgctg gccgtctgcg tcaaccccgg cagcgcgttc aactgctact gggagatgcc
     5881 cttccgcaag aaatgccgca tcaccttcac gaacctcgac gacaaggaga tggtcctttt
     5941 ctaccagatc aactacacgc tgaccgaagt gcccgaggac gcggcctact tccatgctca
     6001 gtttcgccgc tgcaaccccc tgccctacaa gaccgactac accatcgtcg acggcatcaa
     6061 gggcaaagga cagttcgtcg gcacctacat ggcctggggt gtcaacaaca acggctggtg
     6121 gggcgaaggc gagatcaagt tcttcatcga cggcgacacc cagtggccca ccatctgcgg
     6181 gaccggcacc gaggactatt tctgcggctc ctactgcttc atcgtcaata aggggcccgg
     6241 cggcactcgc gagggcaagg gcgagcagta tcaggagtac tcgacgccgt atgccggcat
     6301 gccgcaggtc atccgcccgg acggcgtcta tgacgccaac acccgtttcg ggctgtatcg
     6361 ctggcacatc atggatccgg ttcggttcga gaaggatttg cggattacca tccaggccct
     6421 cggctggcga agccatggcc gctatctgcc tctgcaggac gacatcgcct ccgtcgccta
     6481 ctggtaccag acgctgccga cggctccgtt cccgaagctg ccggataggg atcacctcga
     6541 gatcatctga gcatcacggc gtgccgagca cgcgccgctt ggcttggcac tgccaaggag
     6601 accgctccag tcggatcgtg gccagatctc ggcgagccct ttcgagccag aaccgctctc
     6661 ataggtttgc cgaaagattg ccccctgcat gttttcctga caggccaacg acccgcgcgc
     6721 ctaccagtat cgccggtaga ggtaaaacca gattgcggcg tagatggaaa accagagtat
     6781 gaggctcttg taccacgggc gcggcggggc gtcggcacgg cgcgcgagca gtgccgggct
     6841 ccagttgaac ggctcccatc gctcagccgg tggaggtgga tagcgcagcg agacgatgac
     6901 cgcgccggcg atggtcagca cctgggcaaa gaaggccagg tagagccagt gaagcttgac
     6961 gccggcgagg tggaaacccg cgatgatgac ggcctggatc gcggtaccgc cgaagagggc
     7021 aaaaagaccg ccctggtagt tagcacgctt ccacaggatg ccgaggaaca gggccgtcag
     7081 gaacggcgtc gagagatagg tcacgccctg ctgaaagtac ttgaagatgc cgccgaagtg
     7141 ggcgacggcc ggggcgacga gcgcggcgat aaccagagcc agaagcgagg cgatgcggcc
     7201 ggcccggacg gtctggacat cgtcagcgtg ggggcggagc catttgcggt aaacgtcgag
     7261 agcaaagagc gtcgccgtgg aattggacag agcgctcagc gtggacatca ccgcggccag
     7321 aaagccggca agcacgattc cccgcaggcc ccagtccgga gccagcatcc gcaaggcaaa
     7381 agggaaggcc agatccctgt cgcgcagagg atcggccatg ttcatcacgt gaatccagtg
     7441 gtagacgatc aggccgatga agcaggtcac cagcggccgt atgaaattga tgaagccggc
     7501 gaagatgatg cccatgtagc cgtcccactc agagcgggcc ccgagcacac gctggatcat
     7561 cacctggttg ccggcctgat agaacaggat gacgccgaac atggcggtga tcagcccgag
     7621 gaaaggcgcg gcctcatcgc cggggggacg gtaaaggtgg aatcgatcgg ggcttgcggc
     7681 gatcatcgcg ttccaaccgc catcgactct gcccagggcg gcaaagaaga gcagaacccc
     7741 cccgccaacc agcatgaggc actgcatcgc gtcggtccac atcaccgacg ccaggccgcc
     7801 cttgaccgta taggcggcga cgatcaggac cgtggcccag agtgtgaggt ggaagggcca
     7861 gccggtcagg cccgagatgg cgagtgttcc accgtacagc acgggcacca ggaagatgaa
     7921 cacgtaggcg aagagcatga cgaagctgta gaaatcactg cacagcgtgc catagcggcg
     7981 atggaggaat tccgagacgg tggtgacgcg gttcttgagg tacaccggga tgaagaagat
     8041 catcagcagc gtatagaccg gcaggctgca ccactcccag ttggcgatgg ccatgccgtg
     8101 ctcataggcg gccccgacgg tgccgacgat ctgctcgctg gagacgctgg tggagacgaa
     8161 ggccgagccg atgatccacc atggcagacg gccggaagcc aggaagtagc cgcgggcggt
     8221 cttgtcctgc cgacgcgagg cccagaaacc gacggcaacc acagcggcca gcgaagtgac
     8281 gatgatcgag aggtcaaacg agttaagggc aaactccacg gaatcctccg tgccttggca
     8341 aatccgtgct gaactcgttt tacggacagc ccatctgaac tcagtgcggc caatgatatg
     8401 tgcagactca tgaagcccca agcggtccga tacaccggga tttgcctcag ggcaccccga
     8461 aatgcggcgg cgggcagaag aggcagacgc cgccgtgcgt tgccgctact tcccgacagc
     8521 cgggcagtgt cgtgcacaag gcagccggct tggcgtcgct caccgggcca tggcatggtg
     8581 ctgcccaccg aagggcagta caccaatcag gccttccggt tcggcggcat tcttgcgtcc
     8641 gcatatgtgt ttgctacccg acccggaacg accgtcccgt gccccataat tcgtcgtatg
     8701 gcggctgcgg cacgacgatg ggctcaagtc tgcgcaccat ttgtgaagca ggcagaatcc
     8761 cgatccgatg acggatgatg cacagcgata tccttctcac tgtgctcact cgccgagcgg
     8821 agatgcccca tgaaagccgt caatctgtct tcggacgaca gcgaatggag gcttggcggt
     8881 ctcaacggtt acgcggtcat cctggccgtg gttcacctgg ttgtggcctg tccgatcctg
     8941 gttcttgtcg catgggcggc gaacaacggc ggaatgaccg gcaagtgcgt tccgctcctg
     9001 gctttcgccg tcgggccgtt cgtcgtcttc gcggtttaca gcggtttgtt tcgactcatc
     9061 gccggacgct ggacgctggt acccaacttc gccttccttg cgtgcgtagc cggcgtatgg
     9121 tttgtgggcg cgaccaaaac gtgtcagacc aggatgatca gtgtagcgca agccgagcaa
     9181 acgcatcaga acctgagcag tcaggtcgag ggtcttaccg aaagtctgcg agccttcgtc
     9241 gagaagacgg gtcaataccg gatggaggcg aaacctttgg caaactacct gaaggccgcc
     9301 gaagcggcag gcctcccgcg gcccgcggtc gaggaatgct gcaacacagc gatgacgctg
     9361 ctgaaagagg agcgtcataa acgcgcggtc cgccacgatg cgatctcccg cttgaacgcg
     9421 gccggaggga cccaggcaaa gaccatcacc tcaagaaagg atgctgaaag ccggctgaag
     9481 ctggcccaac agattctcaa ggccgccgag gacgtgcagc agcacatgac ttccggcagc
     9541 gagcgaatcc gggacatcct ggttaaggcg ggcatcaacg gaacgcgatt ggaacaactg
     9601 cgaggttgcg tgcccggata tggggtatct gccgaagaca agagtgcctg tactgcgatg
     9661 ctggagtatg accaaacacg ggtagcgtac ttttctctgc tcgccgacaa gtggggccaa
     9721 tggcaatggg accgcgatcg gtttgcatcg tcggacccgg caatagtgaa ggaatacaaa
     9781 cgactgtctg acgtgctcgg caagactgag gcgaacgctg accttccgcc gaggcaaacc
     9841 cagggcgtca gctccgtgaa caagacgcaa aacccccagc cggtcaagaa cgcgcagaat
     9901 gccaaccccg cgaggaagtc caaaaccgct actccggcaa agaaagccgc gtccgcctcg
     9961 tcgaaggata agagacaaac gatccggtga tggtgcatgg cggtttcgac catatcgcga
    10021 gcagttacgt tgcacgacgt gaaccggttg tcaatccctg tcccccgcgc gatcagcgtt
    10081 tctgcgaagt catcgcgagc ttgaacaagc acgctgagtg agccgccatg cggtaggtgg
    10141 ccggtgggcc ggaggggaga tcctgggtgc ctaattggac gccaggtcgg tccgtgtcct
    10201 ggcacgtcaa ggtccgcacg aaaccggcac ccagatctgc atcccggacc cgcaatgcca
    10261 gatcgaccgg ctcatccgtg gagcggttca ccgccacgac gtacagcgaa ctctcatcgc
    10321 gcatcgccaa ggcggagatg atcggcacgt tcttgacgtc gggggccagg gcgttgaatg
    10381 cggtcgcata cttgtcgccc tggctgtcga agtcgaatgt gtcgcaggtc acctgcgtgc
    10441 ggacggtgtg ggtgcccagg ccgccggcat agaactcgaa gaccttgtag ttgtccttga
    10501 tgcggccgtc ggtgtacatg ataccgcagg ggtaggcccc gaagaggtgg tgcaggtggg
    10561 cccggcggat gccgtggctg atcatcaccc gaaacgcgtc gaccagcagg agttgctgag
    10621 cagcggtgtc ggcggcggcc ggcatgagac cggcctcgtt gtcccagtag tagagctcga
    10681 cgccgtcgat gtcagcttca gcatcagctc cggacagcaa gaagttcacc gcgagggaag
    10741 tggcgccttc gggcaccttg ccgccgaaaa cgattctgtg ccagtggttg gctcgccatg
    10801 ctgtccgact ggcggaatcc gagagcggct cggcaggacg actctcggga ccgagggtgg
    10861 tcagttgagc agagatgcga tcgggatgag gcgtccgaac ccaggccgcg acgcgaatac
    10921 tcgtggcctt tttgtccttc cactcgaagg cctgcgacac cgatatcgac gcgtcggccg
    10981 acgagccggt tctcaggcga aggccgcgtc ctcggcgtcc ggcatcttgg gaaacgacgg
    11041 cggtgctgcc cgcaacaccg tgggtgactc tccagccggt catgtcctgc tcgaaatcga
    11101 gattcttcgc gtccagcttc gacttgcgct gttcacccca acacttgaga ttccactcgg
    11161 tgatttcgat gggtgagcgg taggcgatag cctcttcacg gtatccgccc aggcccgggt
    11221 tggcgacgcc gcgtcggccg tagaggatgt tctgcaggtt gtggtgcgac cagatcagca
    11281 ggtcggtccc ttcgatgctg tagggataga aatggtcctg caccgcatcg atcaggcccg
    11341 cctcgtaaag ctgattcagt tctcgcgtcc agcggacgtt gatgtcgtga ttggtggcct
    11401 ggagcggatc ctgattgttt cccatcaggg ggtagcccac cgcggtgatc cagagtttga
    11461 ggcctttgat cgtcgggtgt tgggcggcgg cctcggccat gagttgccgc atggcctgag
    11521 cgtaggcggc cacggtgggg gcataatgct cgggtggcca gtcggtggtg gcttggcccc
    11581 agttctcgtt gcccatctcg taacaggtga tcggaagtcg accggccagg gcccgctcga
    11641 tggtgtactt gcgaaccaga ttcagcgtct cggccagagt cccgaagtag tagtccgtat
    11701 agccacaggg atttggggcc gtgtgtcgat aacggatgat ctcggccaca ggcgcgcctg
    11761 tcggaggcgg gccgggtaag ccttccgtcc cgccctgcaa gttcagcgtg tagtaaggct
    11821 cagccccgac ggcctcgcag aaaaccagga actgttcgac cgtcatcttc cgttcgttgt
    11881 cggctttcca gtcataacgg tcagcctcgc acccgttggg aaaacggatc gacttgatgc
    11941 cgatcttctt gaccgcctgc atggcttcgg gccgtttgag ggccgccgcg ccgccgtagt
    12001 cgagattgac gccgataatc tcgggccaca cgggcgtttc actgatttga ggcaagatct
    12061 cgacgacagc ggtcgccggc cggctcgcgg tttgagcgat cgagccaccg gttacggtca
    12121 ggaacgccgc gatcaacgcc gttgtgggcc atctccttgc cattcttcat ccccgcagtt
    12181 gtcgtcgggc tctacatcct cgctcctcga agtacttggc ctatcggtat gtcatacagc
    12241 gaatatctcg acgggacgca cttgggagag cgtctttcaa cataccgccg acaacaccaa
    12301 tagacggctc ttcgtgttct cagagaagcg ggggtcagac cccccctgca acctgtgccg
    12361 ccgaagaatc gttgtgagac gacgatacct gctcggacac ggttgaagct gacatggggg
    12421 ccgcaggctt gcccacagcg gaggtgtgct ccacatgccc gccgggcggc aattgtacca
    12481 gcgtgtcggc atcgaccgcc tcatccggac cggcctcggc aaggtattgc ttggatcgcc
    12541 ggtggaagta aagccccaca gcgacgacga gaacgatgag cccgaggaaa ccgatgatgt
    12601 tggtgaccat tcggacatcc gacttgagct gatcaaactg tgacccaaac agccagccga
    12661 gcacgaccag cgtcggaaca ctgatgcacg cggcggagcc gttcacacta atgaatttcc
    12721 agaagggcac cttcataacg ccactggcaa ggaaaaccat cgctcgaagc cccggtagaa
    12781 agcgagccgc gaagatgatc ttgatgccgt ggtgctcgaa aagatgctcg gccagcagca
    12841 gcctggaagg cttcacgatc cgccgcatga aacggtgttc gacgatgtgg tggccgaatc
    12901 gacgacccat gaagaacaga cagacatcgc cggtgagcac acccaacatg ccgacggcga
    12961 tcataatcgg caggacggcc aagccgcgat aacacagcca tccgccggtg agcagaggaa
    13021 tgtcctcggg gattggcatc ccaaagctgg cagcaaccag taccgccagt acgcacaggt
    13081 agggcagttt gtcccagggc attccaatcc tcggtacaac ccgctgtcgc agcttgcgga
    13141 agctctttac aggtcccctc gataactgat tattgctatc gagatttatc cggactatcc
    13201 ttaactcggg gggcgacctt ccgccggttc acggatctta caccggccgg gtggtcctgc
    13261 caaaccgggt tatgatagta ccgagcgagc gaatccggtc gccgaaaggc gcctgatcca
    13321 cgaacggtcc ggagagtcaa ccgccacagt gggaaacaca cataacaaat ggtacgccga
    13381 agggttacgt ttctcgtgca gccagtgtgg caactgctgc acggggccgc ctgggtatgt
    13441 ctgggtcacc cgcgaagaga tccggcgaat ttccgtctat cttggccgcg aagatgactg
    13501 gctcggcaag gagcacctga ggcgcatcgg tttcaggcac tcgctggtgg agaagccaaa
    13561 cggcgactgc gtcttcctca tgtcgacggg caacggaacc cgggggtgct cgatctaccc
    13621 ggtgcggccg ttgcagtgcc ggacctggcc gttctggaca cagaatctca aatcgccttc
    13681 ggcatgggct gcggccggcg aggtctgtcc gggcatgaac aacggccgcc aattcacgat
    13741 cgaccagatc gaggccgttc gcctgaagga ctcttgggag ccgggtgagg agtcgccgtt
    13801 gaaggcagat gctccgtaaa ctcgatgcag gcgtttcatg agtgagaggt tgctaccggt
    13861 ttcggtgtcg gtgctgcggg aacatgcgca acggccggaa atcgtgaggg cgatgcgcga
    13921 gttttacgtt gaggtcgacc gcgagattgc ggctcatgac cccacgtgct ggaacaaggg
    13981 taagtgctgc tgcttcggcc agttcggtca ccggctgtac gtgacctcgc tcgaaatcgc
    14041 gtattacctg gcgacaggca acctcacgcc cccggtcact tccgacgcct gcccccacgc
    14101 gatcgacggc aaatgccacg cccgcgagcg gcggcccttc ggctgccgcg ttttctactg
    14161 cgatccgagc gcccaacact ggcaaggacc gctcagcgag cggcgactga ctcagctcaa
    14221 ggccatgcac gaggcgctgc aagtccccta catgtacgtg gattggatga cggtaatgaa
    14281 gggaatgcag taacggacag gctgccggtc tgttagtctc ttctgtcgcg actggtctgc
    14341 attttacgcg catgtttctg gatcgcagag cgcttgggtt gcggcccgca aaaacgcggt
    14401 ccgccttagt ctgttggtct gttggtctgt tggtctgtca gatggtagcg accacgaaag
    14461 gcatcgaatc ggacccatag cacgtcgagg aacatcctga tcgagtcgcg gaagagtttg
    14521 actttggtat gggagacgtg gctccaatgc acggggatct cgcagatggt caggcccatc
    14581 cgcgacgcga ggtagagcat ctccacgtcg aaggcgaagg tctcgacgcg ttgcagtctg
    14641 aggatcgggt cgatcttgct gcgccgaaag gccttgaacc cgcattgggt gtcgtagatc
    14701 ggcaggccgg tcagcatgcg gacaagtaga ttgaagatct tgccggcggt ctcgcgaaag
    14761 cgggattgcc gcatggcgat caggctacgg tcgagggccc gggaaccgac gacgatgtca
    14821 cactggccgg ccaggatcgg cccgaccaat gcgggcagtt cggacatcgg cgacgataag
    14881 tccgcatcag tgaaggccac gatgtcgccg gttgcgtgga acattccggt tcgcacggcc
    14941 gcgcctttgc cctgattccg ctgatggcga agcaggatga gttgcaccgg agtggctgca
    15001 tgctgccgac gagagccctc gacgatcacg gctgtccggt cggttgagcc gtcatcgacg
    15061 acgatcactt cgcgaaaagc cggccaagcc tcgacataag ccatgacttc ctccaaggac
    15121 cggcctatac acagctcttc gttgtaggct gggatcacca atgagacgct ctgggccgtg
    15181 gtttgaagga gcgattccca aggcgcgatg cggtcagcat gagatagact gatggtgctg
    15241 ttcgaactga ctgccatcac gctaaccgct cacctttgcg tcgggaaggc cggcacgagg
    15301 ccgcgaagca tccccccacg gccagcaggc cgaggcacat gagaaccaga cccgcgcgga
    15361 ggctgccagg ctcaaagcga aactcgaccc gacattcgcc ggccggcacg tggactccgc
    15421 ggaagatccc gttgaccttc agtatatccg cagatcggcc attcacgtaa gcccgccagc
    15481 ccgggtacat catgtcgcac aacacgacca gacccggaac cgaagtagag gtcgtcattg
    15541 tgaccgccga atggccatac cgaacgattc tcacgcggcc gggctcgccc ggcgcagccg
    15601 agggcagacc gattgctgaa gcggcggcct cgaccaaggc gattcgagat gtgtccacat
    15661 gcccctcggc caggtctgca acggcctgct cacatgatgc cgtctcgcgc acctcgtaga
    15721 ccataaacgc ccgagggcat tcgcgcttgt ttcggtagat gttcaagccc tcgcgaaaga
    15781 ccagttccca atcggggtgg tcgagctcat cggacggatc gacaagcaga taacggacgt
    15841 tcagcaatga cagcagaggc gaatcgagcg atccgggatc ctcgaaaccg atgatctggt
    15901 tggaccagag cgcacgctgg ggttcgatcg cgtttatgta atcggcatag tcggcaagga
    15961 tgacggaatc gtagcccccg atatcctgga gtccgtagag catcggaagg ttcggatgca
    16021 agaccatccg ggggccgaaa cggccgatgc gaaacgggcc ggaatcggag cggaggaatt
    16081 caacggccgg ggggacatga tcgagccagg ccggatcagc ctgggtcatg aagccgccgt
    16141 tggcttggaa aggatcagcg gccgtcagta agagacaaag catggccgca accgctgcac
    16201 cgcggggttt ccagacgcgc aagacaggaa gagcaaggac gacggcggcg gccagggcaa
    16261 agatcgtcag acgcagggca ttggcccaca tgtagccggc gacgtcccag gaatcgataa
    16321 agccgttgcc ttgcgagagt cggggaatct ggtcgaaggc ccgttccgcc aggcgatagg
    16381 caagggatgg gaaaacgagc atcaagagca acgcaaggag catgagcgtc ggtacggcga
    16441 tgatcagaac gcctgttatt cgacgttgcc cgcttgcctg gcttgcgacc ggtaccgctg
    16501 catcgagact cccatctcga cactttttga gactctctct catttcgtcg caccacgagc
    16561 cgctcagcct tccaaaccag cgctgtcctc ccattgcggc caggcagcag atggcaaagg
    16621 tgccggcgaa catccagcga aacggagtac gcacctgctc gaaacccggc acggtgtagt
    16681 agaacacggc gtagatcggt gtgccgaagg ccaacagcag tgagagtgca agaaccagga
    16741 tgaaaaacca tcggtagcaa ccgtgggcta acaggccgat cagcatcagc cccagaggca
    16801 tcagccccag gtaaaagccg ttctcgttgt agttcttggt cccgtaggca tagaaatccg
    16861 cgccgcgacg agcttcgatc ggccgcatgc tgcgcgtccg cagatcgaag gccttgtgct
    16921 tggcggggtt tccgaagatg tcgggaacaa tcatttccag cagatgctcg gggcgcaggg
    16981 cgcggttcac catacgctca tagcttgcct cggctgcgcg ggtggtgagg ttcttgactt
    17041 caaggaacgg tagaagctgc ggcccggcga gcatcgcggc caaggctgct gtcgtgaccg
    17101 tggcagcgca gaaccccaga cacctctttg cggaacgagt ttgccgccag agatgccagc
    17161 agcaggcgag ggtgaataac gcagcggtaa agcacgcata gaaggcgatt tcgaagaagc
    17221 ccgccagaat cggcatggcg aagatcactc ctccgagacc gatgcgccac cctgccgcac
    17281 gccggaaagg ggatggcacc atcttcgccg agctttgccg agtgcctgaa ccggccattg
    17341 actcagggat tccgtttgcc ccggcacgag gaaggctgtc gccgaccgca cggaagcagt
    17401 ctcccagacg gaggatccac aggagcatca gcggcagcca gatggccgag ccgataagca
    17461 tgggccagat caactgcatc gccaggaagc cgcacgtcgc gaaggccacg ccgccgacgg
    17521 cgcaaccgaa ggggctcaca ccgatccgcc gtagaaacag gtactgaaac ataccggcca
    17581 gccacaagtg cagaaccgtg tagataacat aggcctgcgg aagcggcgcg agcacgaaga
    17641 tgatgttcag cgggtaaaac gtggaggctt ggccggtggc gtaaagcgga tggccgcaga
    17701 atgcataagg gttccacaaa ggccattcgc cgtcggcgag gcagcggcgc aggagggtct
    17761 tccaggcgta gttctcgtac agcatgtcgc cgatgagcga gttgtgaacc ccggtgaccc
    17821 cggccggcgg cgtctgcgga ggatactgcc atgtcaggtc cgtcggcagg agcaccttgc
    17881 cgccaaagat cgacggccag aaccaaaccg ccagcaccat gcccaggaag atcgcgaatc
    17941 gggggaaggt catgtcagga tcccccccgc aatggccaag tcccaatgac gaaatcccaa
    18001 tgaccggtca atgacgaatg acgaaatccg aatgtcgaaa gaagcccgaa gtccgaagtc
    18061 cgaatgacga gcaagggcgt cagtgctggg cgtagcggcg ggcgtagagg tccggatcgt
    18121 acttggcccg acggccgggg caggtctcgc gcggacagag ctggcagttc tcataatgcg
    18181 tctccgaagg gaaaaagata ccggaaacgc tcttgacggg caccatcaag cagctatcgg
    18241 tcagctccac cccgatcgca ccgcaggtgt cgcccagcag ggtgaagagt tcgcgctgct
    18301 gttccagcgg ccaatcctga agcgagccgg ggtccatggc ggcaaccggg ccaaaggcgg
    18361 ccttatcggt gacatgcttg tgcacgaacc gggcagccgc atggacggcc atttccatca
    18421 atgtctcagc ccagaacctt tcgagcatgt cggtgatcgg cgcagcccaa gccgccatct
    18481 cgcgcccgca ggtcgccaca aaagggaaaa cccgatgcgt ctcccgaaga ttcaccgcca
    18541 acacccggct ggtgaaggtg atgccctcaa cagtgacagt gtcatccgta cggctatcga
    18601 tgtaggcgac ccggtacatg gctcgcggcc gggcgacaac ctgcgcctga ccggccaagt
    18661 caagagcccg tgagtggtag tcatcgaggt cccgcaggtg cagacggtcg agaacggcct
    18721 cgggttcgag atcaaacgcg atgttgtcga ggatgacggg gtcagacatt atgtggttac
    18781 tcaatgcgga gactgcgtcc gctgtcacgg cgcacggtca tgaacgggct gtcagcaacg
    18841 acgggcatca aggtccttca ggcaacgata tcgggcttcg cggcgcggag actcttctcc
    18901 acattggccg acaggttcgc ggcaacgacg aaggccgtga ggcgcctccc ctatcacggc
    18961 acaccagtca tcttagcaca tgttgacagg gcggaccaag ccaaacacag aaatgagccg
    19021 gatgcatcaa ggcttgttgg ccattgagcc acattcagtc gcacggggca tcaaccctac
    19081 gactctgcca gccatggttc ttcaccaagg aacgcaccgg ccggcgcccg cgccggacaa
    19141 acgttcgccg aatccggcct ggtgccagac acgtactgcc aggatgccgg ttccacatat
    19201 tcaatggttg gctcgggcgt agtctcgcat ggcaagaacg ttctcgttag gcgtgcctcg
    19261 gggcacttcg cagccggctc cgacaatgta gcgggcgccc gcctgtcgat gacactcggc
    19321 aagggccgcg tgcacctgct caggagtccc gttgcgcagc acggcaacgg ggttgatgtt
    19381 acccagcagg acctgatcgg gccccatggc ggcgcgagcc tcatccagcg gggccatcga
    19441 gtccagatcg acgatttcgc atttgagtcg gcccatgtcc gtcagcaatg gccgtgtgtt
    19501 gccacagatg tgcaaccgga cgcgcgtgcc catggcgtgg aggccgtcga ccagcttctt
    19561 ctcgtacggc cagaccagtt cgttgtagag ctcggggccg accagcgagg ccgctgcgtc
    19621 gccgacgccg atgatatcca cgcccgcgtc cacctgggcc ttggcgaaac aaagctccat
    19681 ctccaccacg aactcaaaca ggtcccgcac ccaagctggg tcgtcgatca ggtcgagcat
    19741 gagcgcgttc agtccgcgaa ggtcggcgcc catggcgatc ggaccctcga tccagccttc
    19801 gacgattttg tccttccccg ccctttctct gagcagagca actcccttta cccgatcggt
    19861 catacgcccg cccccgaggg gatcaggcat cttgagcgat gccaggtcgg acttgtcggt
    19921 gagccgagcc tcgctctcga caatcgccgg cggctggtcg tcgaagattt caatcgtccc
    19981 accgcagtcg gccgtctcgc gggccgggtc ggagatcacc gacacatagt caaagtcata
    20041 ctgctcggcg gtgcggatct gcccttcagc cagcacgcgg taatcggtcg catactcgcg
    20101 gtatttcgcc ccgatctgat ccgcggcgaa catcatcgta ataggcatca acggcaggca
    20161 atctaacgcc cgcccctcga tcatcgacaa cactcgttcc cggctgttca ttcgtctctt
    20221 ccgtcgtatc aggtgcgcat tccgcgcacg tgtttcggga tcacggtgcg ttttggccgc
    20281 ggctcgcaag aaggcggtcc gccttgggtt ctcctgtcaa gaagtctatc ggacgttggg
    20341 gcaacgttgg tatttgaggt caggccgaag gccaacctcg ccgaaaacct acccggcgtc
    20401 agaataccac acgccgccgg atcgccacaa gcgcgatccg gccgccggtg ctgccggtgt
    20461 gctcgacctt cccgatccgc cgccgtctcg tcgcgatctt tgcccgctcg tcgccggcag
    20521 ggcgtcattg gatcgctact tgttgctgcc gcggatggcc ttttccttca cagcggtctc
    20581 cctgagctgc tcgtatatct tcctggccgc atcgacgcgc ttaagcagtt catcgccttg
    20641 aagcgccaac tgtttgagtt cctgatcacc ctctaacgtc accaagtcct tgcttttggc
    20701 gtcggccacc agtttctcga tttggctgag gctgtcgagt cgcttgtcaa gcaactggtc
    20761 gacatcctcg aacttccacc atctcaggcc ctcacgacgc acgtagatgg gcgtggtgtg
    20821 agcatgacag ccattcgtgg cccgggtcac tgccgccagc cagcaaccgt gcccggcctc
    20881 catgtcgaac tcgaggacga tctcggtgtc gccttccttg gcaggtttgg cgaccctgac
    20941 ctcctggccg aacttgacga cctttacaga ctccggctgc gagaactcgg tctgccccat
    21001 ggccttcaca cgaacgtgga gcttggcgtt gtccttgacc acgatctcat cgccgggcaa
    21061 ggcgtcgttg accgtgaact cgatcatcgg gccattggtc acgaacgtgc gaccccggcg
    21121 cacggcctcg aaccaggcat ccggatcgaa agccttttcc ccaatgcacg catagagccg
    21181 cacctcgccg atcgtgccgc cccacggaac gtcggacccg catgaggcgg tgaccttgaa
    21241 ccccagatcc aggaactggt accacagttc ggtacccaga tggccgaact gcagcacctc
    21301 tgcgaaatcg cacttgccct tgggcacgtt gacgctcata tcgcggtgaa catggaacat
    21361 ccccgagttg acatgggcgt agccccacaa gccgccctgt ccgtgcaccg tgtccgcgac
    21421 gtaatcgtag aggtagtact tgttggtgtc gcgaaccatg ctattgatat tcatcgagat
    21481 ggtgtgcccg atgaactggt gcgttcgcgg acattcctgg cccggcgaga gaacatagtc
    21541 gctattgatg acgcggtaat ccttaccgaa accgcgctgt tcaaagtagg tacgggtaat
    21601 gtcacccatc ttgacgatgt tgcccaggtg tacatcctcc gccaccagcc aggccatgag
    21661 tcgagcggca tcgtcgtcgc ttaggatctg tccatgcacg tggtcgtcgc cggagaacca
    21721 gccgagcttg cgcatgtcga tccatcgctt cggacgataa gtcttgtctg tcaactttag
    21781 ccgtttttct ttgtgctcat gaccatgggt gcttcacaaa ac
//
LOCUS       DAOTPW010000080        21818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2469_length_21818_cov_5.875293, whole genome shotgun sequence.
ACCESSION   DAOTPW010000080 DAOTPW010000000
VERSION     DAOTPW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21818
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2469_length_21818_cov_5.875293"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2866
                     /locus_tag="P5316_09100"
     CDS             <1..2866
                     /locus_tag="P5316_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85155.1"
                     /translation="GCLSHDGLTYYFQTNGQQGNGQLYAINTVDGSVKWTYPTGSTGW
                     EMVSSSPIVTSNGVIIVGNNDGDTYYAIRDDGTQPVLLDTFAVDSAGNARATATIAPD
                     GLLYLPLRTTWIAGNGDGEGPTNTIENLFTAIDITAGATAELWPPAGQAAVALNHAVA
                     LSWQPVLDPTNQFHHYAVYRATTQFKSVDGMSPIAAVDNINTTSYLDSTAVNGIHYYY
                     GVTTVTKSMTEITTVDGIGPRTPRDETDLHVLSLARTPQYPRYLPDYTYYWITEPSGF
                     GPYLFSAATSLGGGQTGATQRWPNTGDTVTYTATVRNRGTNTWSGTLSGTWTVDGAVV
                     GNPSQVVTLAPNATTSFTLLRTWEGLTVARDIGFALNVSDARATNNALVVNTKAVPFL
                     TYVDRSCIENFRENTPVSYPQARTDDMLDWLQRHMTRFNELFAAADCRKRVHYNVLEV
                     LDDVAPDPAVDTTPYAIFPFRYHADEGDPRGSGYYSPTDDIDYGLLHEMGHQLGLVDI
                     YQLDVPAEANQVSGQGYSARADLMHGCSPLISAHSAGAMNHWLDTVHGYYGQYLYQLP
                     AEIRLRILGFDGRPLPGATVKMYQYCERPGQGKVITTQIKAQGLTDANGEYTLPNVPI
                     DPVKVPPAYNGDVLNANPFGYVAVVGTNGLLHFRVEYNGGVDYCWLDITEANVAYWEG
                     QTTTAIFERLLGLGGPVQTRPPDDMTELNAADWAAWAEGSSPENTYVVDDTGRKVVGA
                     GSVKFITDGGFDTYVRYPRSFTARWNLTSATMLKFRVYAENPSPYDFQNGSPWIRLKD
                     AENNYFEYQFYMNGSPYNLLNQALGQWRSYEVPLDASPTTQTGWRRTAVGNPSLANIQ
                     FLEIHADTWDSGFTLWWDGVGFDLPPTVSADLDEDGDVDENDYTIFEQCATAPGVRLL
                     AVDCAIADFDGDSDVDQSDFSVLQRCVSGAGITPPSTCAD"
     gene            complement(2868..5015)
                     /locus_tag="P5316_09105"
     CDS             complement(2868..5015)
                     /locus_tag="P5316_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRR85156.1"
                     /translation="MAMIQHKPTGWNTWDFRGFNRLVFLREGRTDIVVQYAIWDEHVS
                     PPSPDSKQIGHLYETFRWSDVRCLGPHAPMGLPARLEFMAGNVPYAAEATETGGTLRL
                     TVTPLAETRHRIVFMLVAPFGESPAVTSRGSTFGRWRIDLEGATWPRRYFLNIAEPYA
                     VAEPGQAASLIATPAKPMATSGGSFQAYEQEALAGTGALAEAPEAMMRAIYWNTLYDA
                     RRRLVSSPVSRDWCYDWRGVLVFCWDTFFVGAMLSYESRELSRANFEAVTAAVDELGM
                     VPNYYMAHGAASLDRSMPPLGAYMIWKVEQAEPDPAWLRRIYPRLRKWHTYWMKNRDG
                     NEDGLLEWGTNPTPTYEFPQLLPYNPSLQHTAKCAMYESGLDNSPMYDDVPFNESANT
                     FELADVALNSYYAMDCEALASMAEALGKPKDAAAYRREYERMRRRINDRLWDEEHGIY
                     ANRHWDGRFSNRWSPTSFFPLIAGVADERQAERLVREHLLNEAEFWGRYVIPAIARND
                     PAFGDNDYWRGRIWGPFNLLVAEGLRRYRFDDVAADLARRGLEMFMYNWRKDGGVYEN
                     YNATTGEGGDVWNAARLYHWGGLMAYIAVQELIDVEATGFLRFGSVSFPSAGVRNVRI
                     GTDRYEIRLDEGMKVTRNGEPFIECDSRMIVRIPLIAAPDQPIEVSAPAAGSLTLSTP
                     QQLSRSCRINGRRTVHAERTAAGLVYRWPAAAS"
     gene            5070..6197
                     /locus_tag="P5316_09110"
     CDS             5070..6197
                     /locus_tag="P5316_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR85157.1"
                     /translation="MTHKERVARTLARKQPDRVPIYDKFWFEVEREYREQLGCPFVFH
                     PERSAFDWGAVSPETQATTLWELFDMDITEVAWPDFRLRFIEPEILEETDEWILLRDG
                     NWAELRWWKHKMGTPEHVRFGIDTPDKWAEVKGLLTPSRNRVRWNEFWPLYQRARKAE
                     RFVCYGVADPFENLKDVVGHEIMLLSMIERPEWVHDLFETYTDLHIKMLEIVEAEGMV
                     CDGAFVYGDMAYRNGPFMSPAHYREFVQPCHKRLFDEFRKRGMPIIFHTDGDVRVVID
                     DLIDSGIDALNPLEAKANMDVRELAPKYGRRLGFCGNIDVTVLMSNDRDRIREEVRSK
                     LAAAMPYCGYIYHSDHSIPPGVTLETYQALLAEVREAGRYE"
     gene            6210..7259
                     /locus_tag="P5316_09115"
     CDS             6210..7259
                     /locus_tag="P5316_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR85158.1"
                     /translation="MTRREIVERAITFSKPPRLPMKFDVVGVNDCYDVWTHDPTGWTW
                     AFKERAADEWGCVWERTEVSNTGQVVGHPLQDWSRLADYQWPDPDDPRRYRDFQEQLA
                     GADDRFVMFCFGNGIFERLHMIHGMTETLIDFYEEPDKIHEVIERILDHHLRVFRNCA
                     RIASGRMHAAAMADDWGMQDRAFISTELFRQFFKPRYKRWFDEIKAAGMHPWMHSCGH
                     VNELIEELIDCGLEVINLQQPNTVGIEDIGRRYRGRICFESIVDTQSTLPRGSYEEIR
                     AQAHRLVDEWGTPDGGFIASDYNDAEAIGVTTDRRRVMFAAFAEKGGYPNYQQILEQT
                     REALVGHSWGREASA"
     gene            7323..9536
                     /locus_tag="P5316_09120"
     CDS             7323..9536
                     /locus_tag="P5316_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85159.1"
                     /translation="MVRPQIILALAWQGVMLFSVGLGAQPVVSGRSTPGPMAATRTVP
                     VSVVTATDEHRLQQLKDHEPLGANILRRPFDPTYESVADVLPAMSKPREVVGVKDHRY
                     EIGVEYDGTIQIPDDTQTWEQTGTVAYFEVASPAVRFGTGGCTKRLLQGYLPIVVADW
                     RHEQFVYQETVFGWSETMSPEKDLYAYCRLQASNRSDTPHDVDIVLRCAPNTVVKEPL
                     RKSLRVAAQGTAGVCFKMALPLEAASVSEVDAGEFERRLDEVRQSWTAILNSGTVVNT
                     PEPRINDACRAWLAYNYLDVDKKGDVYEPHDGAGFYEQVYGYSAALYCHALDLWGRHE
                     DSRRYLESLLTFLRPDGLFFVNYGLPDHGALLFALCEHHRMTGDDAWLRSMAPAMVKM
                     CDWTIAQRRLPTTTTADPKPVTHGLIRFTPYADYQERTVNYYADAYCCIGLEHTAAAL
                     AAVGMENESRRLTAEATAYRNDILRSMDAAAFERDGMRLLPMEPDTHRLLKSTNYRAG
                     GYYGLIASMFLESEFLPAADERARWVVDALERRGGLIMGMCEFDGGVDHAYTYGYWLN
                     CLRRDEVRRALLGFYATLAYGMGRDTYCGVEVTQIMTGEPTPTTPHLYSGTQQLRLLR
                     MMLLREEGERLLIGHAIPQAWLEAGKRIEVRNAATAFGPLSFVIESDEDASQIRIRLD
                     PPAARLPASIDIRLRHPAGRNLTRVTVNEQPTTSFTRDTLSLPPQRGAIQIRLAY"
     regulatory      10117..10389
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10629..12137
                     /locus_tag="P5316_09125"
     CDS             10629..12137
                     /locus_tag="P5316_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85160.1"
                     /translation="MYRRKLLITGMGLIAAAWGGPTLAGPYSGPADTAHGVDPAIPAG
                     SPLFVEWADRILPLGAGTYFAPRGSTSISLTGYNSLGDLDAAQIAGGDSPGYLTVTFP
                     TGIRDGAGHDFAVFENGFAYGSPNGLFAEFAYVEVSTNGVDFVRFDGISTNTAPVAGS
                     GAFAGYDVSNVYNLAGKHAAGYGTPFDLAELINKPLVLSGQVDLNNIRFVKLVDIPGN
                     GSFLDSLGNPILDNWLTTGTGGFDFRLPAGQGIGVINVAPEPASLALIGLTCALLTAR
                     RKSRRASLRLFAGVVLCMGLLKTGPVQAVTFDDIDYWIGKGSNRAGFIIDWNDGTSAE
                     SLVWGYRWDGTKKGEDMLRDIVAADARLFALVSGSGGYGAALYGMGYDLDNDGVFGVS
                     PALSFDAGGMAMGSPNDSRVPTDTADHWKEGWMSAGYWSYWLSGSGDSPSWGFSGTGM
                     SGRTLTNNCWDGWSWAPGFSGSAPSEPVPAQVPDPASSSLFLTGSLCVLRRR"
     gene            12213..13304
                     /locus_tag="P5316_09130"
     CDS             12213..13304
                     /locus_tag="P5316_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85161.1"
                     /translation="MARTIVTCAATILVAMGSVAVAYDYVYSPSDFAVEVVEYQQGGP
                     VPVDWIWNTPFNLPEASLGRPTIDTTGDWWTGPPTDPITVVPVYPAFRVWEMVSIGEG
                     GRLVLAFDHAVENDPRNPCGIDFIIFGNAFQVINGGSYWRNGDPSQCIIGTSSMWAEP
                     GTVSVAQHYDPAYPEMTVWYTFDNPPGADMFAPTLGRIYDPDNAPSGLPHNGWWGAPT
                     HPLIPFNPNLGPADFVGHSLAHYARKYGYCAGGTGFDLDDVGLQWFRYIRIDNPVGSG
                     MTPEIDAVADVDPDAPAPDFDCDTDVDVDDMAFFESCATGPGLGPPAAGCERTDLDQD
                     GDIDQADFAVVQRCYSGPDVAADPDCKGL"
     gene            13322..14134
                     /locus_tag="P5316_09135"
     CDS             13322..14134
                     /locus_tag="P5316_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HRR85162.1"
                     /translation="MNGRQRERRGFTLIEVLVVAALIAMLLAILLPSLGRARHLARSV
                     QCLSNLHQMGVAAHSYATTHGRFPPYLYGSPDGSVSYGWDLRVHYRWEGGQRKATVKP
                     GLLWQGKTIDEINQCPSYQGGDNWVDYSHTGYNYNSSYVGYCEYYAEVLEWPPVPTGK
                     LVVKENPARPEDIRRSAGCTLFGDGEYVAGANKFMRSPFKGRDGSFSGRSAGTQGYRH
                     LKRTNVVFADGHAESWIKRFADTYAFDQPNVRPHGDVPTGFLSADNGLYDLD"
     gene            14202..14720
                     /locus_tag="P5316_09140"
     CDS             14202..14720
                     /locus_tag="P5316_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRR85163.1"
                     /translation="MPQGYVHVYTGNGKGKTTAALGLALRAVGAGHRVFIAQFAKGMD
                     SSESRAFARLSDRVVLRQFGLPHFVRGQATPADVTIASLGLREVESAVHGGEYDMVVL
                     DEANVAVKLGLLPLDWLLQLIVEKPEPLELVITGQAAHPRLIEIADLVTEMREVKHYF
                     RRGVLARIGVDM"
     gene            complement(14903..16360)
                     /locus_tag="P5316_09145"
     CDS             complement(14903..16360)
                     /locus_tag="P5316_09145"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRR85164.1"
                     /translation="MNEKKLTNEVGAPVADNQNVLTAGPRGPMLLQDVWYLEKLAHFD
                     REVIPERRMHAKGSGAYGTFTVTHDITRYTKAKIFSKVGKKTDVFVRFSTVAGERGAA
                     DAERDIRGFAIKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAVKRDPKTNLRSARN
                     NWDFWTSLPEALHQVTITMSDRGIPYSYRHMNGYGSHTFSLINAKGERFWVKFHLKTR
                     QGIKNLTDAEAEAIIGKCRESHQRDLFESIEKGDFPKWDMKIQVVPEQGAAKFPFNPF
                     DLTKVWPHKDYPLIDVGVLELNKNPENYFAEVEQAAFNPASIVPGIGFSPDKMLQGRL
                     FSYGDAQRYRLGVNYHLIPVNRARCPVQCFHRDGRMRVDGNYGGTLGYEPNSYGEWQE
                     QPEFREPPLAIEGAADHWSHRQDDDDYYSQPGALFRLMSPQQQEALFGNTARAMGDAP
                     REIKIRHIGNCLKADPAYGEGVAKALGIPLSEVPK"
     gene            complement(16440..16838)
                     /locus_tag="P5316_09150"
     CDS             complement(16440..16838)
                     /locus_tag="P5316_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRR85165.1"
                     /translation="MEDLIAILRRHGIHPTPQRLAVARVVLNRESHPSAEEAWAKVRK
                     RCPTISRATVYNTLNLFVKKGLMRQQVLKEGLALFDAEVRPHHHFIDEQTGKVYDVPW
                     EALRVTGAESLRRFTVREYQVVLRGRRQKK"
     gene            complement(17142..17624)
                     /locus_tag="P5316_09155"
     CDS             complement(17142..17624)
                     /locus_tag="P5316_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85166.1"
                     /translation="MLPIVHVAPHWSWVCVMVKRMLGGTETDPPRAGGCNVCGIVFLL
                     AVLLAFPGLAWYAGSAVKQVISRGDAESRLGRMRDDVGRERRGGGNGEGRGQISGSMS
                     LGNIRAATGVTADTLKSDLGLPSDVSDQERVGRLSRRHGFNVAQIRDIVAKRVSKPGE
                     "
     gene            complement(17576..18631)
                     /locus_tag="P5316_09160"
     CDS             complement(17576..18631)
                     /locus_tag="P5316_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR85167.1"
                     /translation="MKTVVRLPCRNLTSAYTGFVWFSVLLILQSGVGCTGPATQTDAR
                     RPFSIVMLPDTQIYSEKRPDLFFAQTNWIKLNRDKENIVFVTHVGDLVQNHGYEPSQW
                     RVADEAMAVLDGVVPYGIAIGNHDYDSDEGARKGIATMYLRYFDPERRFKGRPWYGGA
                     SPNGLNSYQLFFGGGVDFVILHLELDAPDDALDWARGVLDRHPTRSAIITTHSYLRGD
                     AGVGRQTQRECRKDGNSGEEMWQKLIRRSPQVFMVLCGHIGSVGEYHQISRNDAGADV
                     LEMLADYQERENGGNGRLRLIRFVPADNEIQIRTYSPVLDQFETDAKSQFVVPWNRSP
                     MPCCRSFTLHRTGRGCA"
     gene            complement(18943..20112)
                     /locus_tag="P5316_09165"
     CDS             complement(18943..20112)
                     /locus_tag="P5316_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR85168.1"
                     /translation="MAPDPLTPYGNRPMVTPIVNAVNYEYLSFDVLRQITDGEIDGYT
                     YHRDDNPTVREVERKIAALEGAEDCVVCTSGMAACTMVYLTYLKTGDHLIIFHDTYGA
                     NYKVSLILERMGVQITWLDADEADRIGKHIRPNTQMIFLETPSNPLCKIIDVGAVRQA
                     ADSVGAMLVVDNTFATPYHQHPLELGADLVVHSATKALGGHNDLMAGAIAGSKEHYNT
                     LWFTRQAIGSTLDAYSAFLLDRGLKTFELRAEAISRRAQIVAEFLAQHPKVTRILYPG
                     LTSHPGHAVAVRQMHGGFGGMLAFDVGEDEEDAKRFVAALKTIYHAVSLGSTESLICI
                     PYLTTMLYLPPERRTMFGVRKNTVRLSIGIEPAEKLVKDLKQALACVPERAVQNF"
     gene            complement(20416..21468)
                     /locus_tag="P5316_09170"
     CDS             complement(20416..21468)
                     /locus_tag="P5316_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR85169.1"
                     /translation="MRELVVISGKGGTGKTSIAASLAVLAGRCVVADCDVDAADLHLV
                     LSPQVKECHDFYSGHQAVIRQADCTSCGMCLAHCRFGAVVRRFPEGSSGVIVGSTSYD
                     CHSCAYCERSCPARDNDMIQAMSEAIGETRQGALAVDPIACEGCGVCVQVCPVRAIDF
                     PERLCGEWMVSETRCGPMVHARLGVASENSGKLVSIVRGNARRIAEERSIELIIIDGA
                     PGLGCPVIASITGAGLVLVVTEPTLSGEHDLRRVIQLTRHFGIVATVCVNKWDLNPEM
                     SELIEAYCREHFIPVAGRIRYDRAFTEAQIDARAVVEASDHGASEDIRRLWDAIRDGF
                     DTLTLQEDPHHGTSSS"
     gene            complement(21465..>21818)
                     /locus_tag="P5316_09175"
     CDS             complement(21465..>21818)
                     /locus_tag="P5316_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HRR85170.1"
                     /translation="VRGADCVVLVTEPTPFGLHDLKLAVEMVRTLNLPPGVVVNRADA
                     GNRATWEYCSGNQIPVLAEIPDDRRVAEAYSRGQLAALLSQEYRDRFRSLLREVTTLA
                     TSPRPHAVMSGGGSR"
BASE COUNT         4150 a         6917 c         6611 g         4140 t
ORIGIN      
        1 cggttgcctc agccacgacg gtcttaccta ctacttccag accaacggtc aacagggcaa
       61 tgggcaattg tatgccatca acacggttga cggctcggtc aaatggacct accccaccgg
      121 cagcaccggc tgggagatgg tctcatcctc accgatcgtg acctccaacg gcgtcatcat
      181 cgtcggtaac aacgacggcg acacctatta tgccattcgc gacgacggca cacagcccgt
      241 gctgctggac acgttcgcgg tcgactcggc cggcaacgcc cgggctacgg ccaccatcgc
      301 cccagatggc ctgctttatc ttccgcttcg caccacctgg atcgccggaa acggcgatgg
      361 tgaaggcccc accaacacga tcgagaacct gttcaccgcc atcgacatca ccgccggcgc
      421 caccgccgag ttgtggcctc ccgcaggcca ggccgccgtg gccctgaacc acgccgtagc
      481 cctctcctgg caaccggtcc tcgatccgac caaccagttc catcactacg ccgtctaccg
      541 cgcgacgact cagttcaagt ccgtggacgg catgagcccc atcgccgccg tcgataacat
      601 caataccacg agctatcttg actccacggc ggtaaacggc atccactact actacggcgt
      661 taccaccgtc accaagagca tgactgagat caccaccgtc gacggcatcg gtccacgcac
      721 gcctcgcgac gagacggacc tgcacgtgct cagcctcgcc cgcacaccgc agtacccacg
      781 atacctgcct gactacacct attactggat caccgagcca tccggcttcg gtccgtatct
      841 cttctcggcg gctacctccc tcggcggggg gcagaccggc gcaactcaac gctggccgaa
      901 caccggcgac acggtcacct acacggcgac ggttcgcaat cgcggcacaa atacctggag
      961 cggtaccctt tcaggcacgt ggacggtgga cggggcagtg gttggtaacc catcacaagt
     1021 tgtgacgctc gcgccgaatg ccacgacgag tttcacgctc ttgcgaacgt gggagggcct
     1081 gaccgttgct cgcgacatcg gttttgccct caacgtctca gacgcccgcg cgacgaataa
     1141 cgcactggtc gtcaacacta aggcggtacc cttcctcacc tacgttgacc ggtcctgcat
     1201 cgaaaacttc agggaaaaca cgccggtcag ctacccgcag gcccggaccg acgacatgct
     1261 cgactggctc cagcgacaca tgacgcggtt caacgagttg ttcgccgccg ccgactgccg
     1321 caagcgcgtg cactacaacg tgctcgaggt cctggacgac gtcgccccgg atcctgcggt
     1381 cgataccacg ccttacgcca tcttcccgtt ccgctaccac gcagacgagg gagatccccg
     1441 cggctccggt tactacagcc cgaccgatga cattgactac ggactgctgc acgaaatggg
     1501 gcaccaactt ggcctggtcg acatctatca gctcgacgtc cctgccgagg cgaaccaggt
     1561 ctccgggcag ggctacagcg cgcgggccga cctgatgcac ggttgcagcc ccttgatctc
     1621 cgcccatagc gccggagcca tgaaccattg gttggacacg gtccacggtt attacggcca
     1681 atacctgtac cagttgccgg ccgagatccg gctgcgaatc ctcggattcg atggccggcc
     1741 tctgcccggc gcgacggtca aaatgtacca gtactgcgag cgcccgggac aaggaaaggt
     1801 cattaccacg caaatcaagg cccaggggct gaccgacgcc aacggcgagt acaccctgcc
     1861 caacgtcccc atcgatcctg tcaaggtgcc gccggcctac aacggcgacg tgctgaatgc
     1921 caacccgttc ggctatgtgg cggtcgtggg aaccaatggg cttcttcact ttcgcgtcga
     1981 gtacaacggc ggggtggact actgctggct cgatatcacc gaagcgaacg tcgcctactg
     2041 ggaaggccag accacaacgg cgatcttcga gcgactgctc ggtctgggag ggccggtcca
     2101 aacccggccg cccgacgata tgaccgaact gaacgcggcg gactgggcgg cctgggccga
     2161 gggttcgagc cccgagaaca cctacgttgt tgacgatacc ggccgtaagg tcgtcggcgc
     2221 gggatcggtg aagttcatca ccgacggggg cttcgatacc tacgtccgtt acccgcgaag
     2281 cttcacggcc cggtggaacc tcacgtccgc caccatgctg aagttcaggg tgtacgccga
     2341 gaaccccagt ccctacgact tccagaacgg cagcccgtgg attcgtctca aggacgccga
     2401 gaacaattac ttcgagtacc agttctacat gaacgggtcc ccgtacaacc tgttgaacca
     2461 ggccctgggg cagtggcgat cctatgaggt gcccctggat gcgtctccca cgacccaaac
     2521 gggatggcgg cgcacggccg tcggcaatcc cagcctggcc aacattcagt tcctggagat
     2581 ccacgccgac acgtgggatt ccgggtttac gctctggtgg gacggcgtgg gatttgatct
     2641 cccgccgacg gtgtccgctg atctggacga ggatggcgac gtcgatgaga acgactacac
     2701 gatcttcgag caatgcgcga ccgccccggg cgtaaggctg ctcgcggtgg actgcgcgat
     2761 tgccgatttc gacggcgaca gcgacgtcga tcagtccgac ttcagcgtat tgcagcgatg
     2821 cgtcagcgga gccggcatca cgccgccttc aacctgcgcc gattgactta cgatgccgcc
     2881 gccggccacc ggtaaacgag acctgcggcg gtgcgctcgg catggactgt ccgtctgcca
     2941 ttgatgcgac aggatctcga caactgctgg ggtgtcgaga gggtcaaact gcccgcggcg
     3001 ggcgcagaga cttcgatcgg ctggtcgggc gcggcgatga gagggatccg tacgatcatt
     3061 cgcgagtcgc actcgataaa gggctcccca ttgcgagtga ctttcatccc ttcgtcaagc
     3121 cggatctcgt atcggtcggt gccgatccgg acattgcgga cgcccgcgct gggaaatgac
     3181 accgagccaa aacgaagaaa gccggtggcc tccacgtcga tcaactcctg gacggcgatg
     3241 taggccatca gtccgcccca gtgataaagc cgggcggcgt tccagacgtc gcccccctcg
     3301 ccggtcgtcg cattgtagtt ctcgtacacc ccgccgtcct tgcgccagtt gtacatgaac
     3361 atctccagcc cacgccgggc aagatcggcc gcaacgtcat cgaagcggta ccgtcgcagc
     3421 ccctcggcga ccaggagatt gaagggcccc catatccgcc ctcgccagta gtcgttgtcg
     3481 ccgaaggccg ggtcattccg cgcgatggcc ggaatgacgt aacgtcccca gaattccgct
     3541 tcgttgagca gatgttcgcg gaccagccgc tccgcctgcc gctcatccgc cacgcccgcg
     3601 atcagcggaa agaacgacgt cggcgaccag cggtttgaga atcgcccgtc ccaatgccgg
     3661 ttcgcgtaga tgccgtgctc ctcgtcccac aaccgatcgt tgatgcgccg acgcatcctt
     3721 tcgtattcgc gacgataagc ggccgcatcc ttcggcttgc ccagagcctc agccatcgac
     3781 gccagcgcct cgcagtccat cgcgtagtac gagttcaacg ccacgtccgc caattcgaac
     3841 gtgttcgccg actcattgaa tggaacgtcg tcgtacatcg gggagttgtc cagacccgat
     3901 tcgtacatcg cgcatttggc ggtgtgttgc agcgacgggt tgtacggaag cagttgcggg
     3961 aattcatatg tcggcgtcgg gttcgtgccc cattccagca ggccgtcctc gttgccgtcg
     4021 cggttcttca tccagtaggt atgccacttg cgaagccgag gatagatgcg ccggagccac
     4081 gccggatcgg gctcggcctg ctcgaccttc cagatcatgt aggcccccaa gggcggcatt
     4141 gagcggtcga gcgaagcagc cccatgggcc atgtagtagt tggggaccat gcccagctca
     4201 tccacggccg cggtgacagc ctcaaagttg gcgcgggaca gctcccttga ttcatacgag
     4261 agcatcgctc ccacgaagaa ggtgtcccag cagaacacca gcacgccgcg ccagtcataa
     4321 caccagtcgc ggctgacggg agacgacacc agacgccgcc gtgcgtcata cagggtgttc
     4381 caatagatgg ctcgcatcat ggcctccggg gcttcggcca gtgctcccgt accggcaagg
     4441 gcttcctgtt cataagcctg gaaggatccc cccgacgtcg ccatcggctt ggcgggggtg
     4501 gcgatcaggc tggctgcttg gccgggttcg gcaaccgcgt agggttcggc gatgttgagg
     4561 aagtaacgcc gcggccaagt ggcgccttcc aggtcaatcc gccaacggcc aaacgtgctg
     4621 ccgcgagaag tgaccgccgg cgactcgccg aacggcgcca cgagcatgaa aacgattcgg
     4681 tgccttgttt cggccaaagg agtcaccgtc aaacgcaggg tcccgccggt ctcggttgcc
     4741 tcagccgcat agggcacgtt gccggccatg aattccagcc gggccggcag acccatcgga
     4801 gcgtgcggac cgaggcagcg cacgtcgctc catcggaaag tctcgtacaa gtgcccgatc
     4861 tgcttgctgt ccggcgacgg cggtgatacg tgctcatccc aaatcgcgta ctgaacgaca
     4921 atgtccgtcc ggccctcccg caggaaaacc agccgattga agccgcgaaa atcccaggtg
     4981 ttccatccgg ttggcttgtg ttgaatcatg gccatgagta tacgacgacc gctggcatgg
     5041 tccagcctcc ctcatgtaca atagcccgca tgactcacaa agaacgcgtt gcccgaaccc
     5101 tcgcccgcaa gcagcccgac cgcgtcccca tttacgacaa gttctggttc gaggtcgaac
     5161 gtgagtaccg cgaacaattg gggtgcccct tcgtgttcca tcccgagcga tcggcattcg
     5221 actggggggc ggtttcgccc gagactcagg ccaccaccct ttgggagctg ttcgacatgg
     5281 acattaccga agtcgcgtgg cccgacttcc gcctgcggtt catcgaaccg gagatcctcg
     5341 aagagaccga cgagtggatc ctcctgcgcg acggtaactg ggccgaactg cggtggtgga
     5401 aacacaagat gggcacgccc gagcacgtcc gcttcgggat cgacaccccc gataaatggg
     5461 ccgaggttaa gggcctcctg acgccctcgc gaaaccgggt gcgatggaat gagttctggc
     5521 cgttgtacca gcgggcccgc aaggccgaac gattcgtctg ctacggtgtg gccgacccgt
     5581 ttgagaacct caaggacgtg gtcggccatg agatcatgct gctctcgatg atcgagcgcc
     5641 ccgaatgggt gcacgatctt ttcgagacct acacggacct gcatatcaaa atgcttgaaa
     5701 tcgtcgaggc cgagggcatg gtctgcgacg gtgccttcgt ctacggtgat atggcctatc
     5761 gcaacgggcc gttcatgagt cccgcgcact accgcgagtt cgtccagccg tgccacaaac
     5821 ggctgttcga tgagttccgc aagcgaggaa tgccaatcat ctttcacacg gacggcgacg
     5881 tgcgggtggt catcgacgat ctgatcgact ccggcatcga cgccttgaac cccttggagg
     5941 ccaaggcgaa catggatgtt cgcgagttgg cgccgaagta cggccggcgc ctcggattct
     6001 gcggaaacat cgacgtcacc gtcctgatga gcaacgaccg cgaccggatc cgcgaagagg
     6061 tccgctccaa gctggccgcg gcaatgccgt attgcggcta catctaccat tccgaccact
     6121 cgattccgcc cggtgtgact ctggaaacgt atcaggccct gctggccgaa gtccgagagg
     6181 ccggacggta cgaatagagg ctccctctca tgaccagacg cgagattgtt gaacgggcga
     6241 tcacgttctc gaagccccca cgcctgccga tgaagttcga cgtggtgggg gtcaacgatt
     6301 gttatgacgt atggacgcac gacccgaccg gctggacctg ggccttcaag gagcgcgcgg
     6361 cagacgaatg gggttgcgtg tgggaacgca cggaggtctc gaacaccggc caggtcgtcg
     6421 gtcatccgct gcaggactgg tcgcgtctgg cagactatca atggcccgac cccgacgatc
     6481 cccgccgata ccgcgatttc caggagcagc tcgcaggtgc cgacgaccgt ttcgtcatgt
     6541 tctgtttcgg caacggcatc tttgaacgcc tgcacatgat tcacggcatg accgaaacgc
     6601 tcatcgactt ctatgaggag ccggataaga tccacgaagt gatcgagcgg atcctcgatc
     6661 accacctccg ggttttccgc aactgcgcgc gcatcgccag cggccggatg cacgcggcgg
     6721 cgatggccga tgactggggc atgcaggatc gggccttcat cagcacggaa ctctttcgac
     6781 aattcttcaa acctcgatac aagcgatggt tcgacgagat caaggcggcc ggcatgcatc
     6841 cctggatgca ctcgtgcggg cacgtcaacg aactgatcga ggagctgatc gactgtggcc
     6901 tggaggtcat caatcttcag cagcccaaca cggttgggat cgaagacatc ggccggcgat
     6961 atcggggacg aatctgcttc gagtcgatcg tggataccca gtccaccttg ccccgcggta
     7021 gttacgagga aatccgcgct caagcgcatc gcctggtgga cgagtgggga acgcccgacg
     7081 gcggcttcat tgccagcgac tataacgatg ccgaggccat cggcgtcacc acggaccgcc
     7141 gccgggtgat gttcgccgca tttgccgaga agggcggcta tcccaattac cagcagatcc
     7201 ttgagcagac ccgcgaggcc cttgtgggcc attcctgggg ccgcgaggca tccgcgtaag
     7261 cacggccggc ggccaatctg cgaaccttcg gaacaagcgg ccggacagaa ggaggagaca
     7321 caatggtcag accgcaaatc atcctggcac tggcgtggca gggcgtgatg ctcttctccg
     7381 tcggtcttgg ggcccaaccc gttgtctcgg gtcggtccac tcccggccct atggctgcaa
     7441 ccaggacagt gccggtctcc gtggtcacag cgacggatga gcaccgcttg cagcaactca
     7501 aggatcacga accgctgggc gcaaacattc tccgccggcc gtttgatccc acgtatgaga
     7561 gcgtcgccga tgtgctgccg gcgatgtcga agccgcgcga ggtcgtgggc gtcaaggacc
     7621 accggtatga gatcggggtc gaatacgacg gcacgatcca gatccccgac gacacccaga
     7681 catgggagca gaccggaacc gtcgcgtatt tcgaggtggc atcgccggcg gtgcggttcg
     7741 ggacaggcgg gtgcacgaag cgcctgcttc agggttacct tcccatcgtg gttgccgatt
     7801 ggaggcacga gcagttcgta tatcaagaga ccgtcttcgg ctggtccgaa accatgagcc
     7861 ccgaaaagga tctgtacgca tattgccgtt tgcaggcctc aaaccggtca gatacgccgc
     7921 acgatgtgga catcgtattg cgttgtgcgc ccaataccgt cgtgaaagaa ccgctgcgca
     7981 agagcctccg tgtggccgct caaggcacgg ccggggtctg cttcaaaatg gctctgccac
     8041 tggaggcggc ctcggtgtcc gaggttgacg ccggcgaatt cgagcggcgc ctcgacgagg
     8101 tcaggcaaag ctggaccgcg atcttgaact cgggcacggt cgtcaatacc ccggaaccaa
     8161 gaatcaacga cgcctgtcga gcgtggctgg cgtacaatta cctcgacgtg gacaagaagg
     8221 gtgatgtgta tgagccgcac gacggcgccg gcttctacga acaggtctac ggttactccg
     8281 ccgcccttta ttgccacgcc ctcgatctgt ggggacgtca cgaggattcc cgccgatatc
     8341 tcgaatcact gttgacgttt ctgcggcctg acggtctttt cttcgtgaac tacggcctgc
     8401 cggaccacgg ggccctgctc ttcgccttgt gtgagcatca tcgaatgacc ggtgacgatg
     8461 cgtggttgcg aagcatggcc ccggcaatgg tgaaaatgtg cgactggaca atcgcccaac
     8521 gccgcctgcc gaccacaacc acggcggacc cgaaacccgt gacccacggc ctgatccgct
     8581 tcactcccta cgcggactat caggagcgga cggtgaacta ctatgccgac gcttactgct
     8641 gcatcggtct ggaacatacg gccgcggcgc tggcggcggt cggcatggag aatgaatccc
     8701 gacgactcac cgccgaggca accgcctacc gcaacgatat tctgcgctcc atggatgctg
     8761 cggctttcga acgcgacggc atgagactgc tgccgatgga gccggatacg caccgtctgc
     8821 tcaagagcac gaattacagg gcaggcggct actacgggct gatcgcgtcg atgttcctgg
     8881 agagcgagtt cctgcccgcc gccgatgagc gcgcccgttg ggtcgttgat gccctcgagc
     8941 ggcgcggggg cttgatcatg ggcatgtgcg aattcgacgg cggcgtcgat cacgcgtaca
     9001 cttacggcta ctggttgaac tgcctgcggc gggatgaggt gcgccgagcg ctgctgggct
     9061 tctacgcaac gttggcctat ggcatggggc gcgacacgta ttgtggcgtg gaagtcacac
     9121 aaatcatgac cggcgaacct accccgacca caccgcatct ctactcgggc acacagcagc
     9181 ttcgcctcct gcggatgatg ctgctgcgcg aagagggcga gcggctgctc atcggacacg
     9241 caatccccca ggcgtggctg gaggccggca aacgtatcga ggtccgcaat gccgccaccg
     9301 ccttcgggcc gctatcattc gtgatcgagt ccgacgagga cgcgagccag atccgtatcc
     9361 ggcttgatcc gcccgccgcc cggttgcccg cgtcgatcga catccggctt cgccaccccg
     9421 cgggccggaa tctgaccaga gtcaccgtga acgagcagcc gaccaccagc tttacccgcg
     9481 atacattgag tctcccgccc caacgcggag caatacagat ccggctggct tactgactgc
     9541 cgtccgggcg cccggctctg gagccgcggg gaatcaacat cagtgggccc gcttggcggg
     9601 cggcctctgc cttcggcaat ggcggtgtct tcggatcgaa cctctgcaaa tcagggcgaa
     9661 caaccgcggc ctaaacctgt cggaacccca cacacccggt tcatggagca tttgggccgg
     9721 tggatgaggg ctggcaggct cggcgaggcc ccgcgaaccc cggtgcaaga gttccagaag
     9781 aacaagggtg cagacctcgt cccgtcggct cgcacggtcg acgccgaaga acatcccatc
     9841 tacctgccgt gcgtgacgat cccgacaagg cccaggaggt tccgtttcag tgattgaggc
     9901 tggatctgcg ccgacggttg cggctaagcg acgaggtcgt ccaaatgtag tcacgacttt
     9961 tggacacaaa aggccctggc gctatcgcca aagccgttct cggctcacaa ctgttcatct
    10021 tgggctgggg aggcgatgcg gcgccgttca gcgattccgc aacgtgattg ttctgcaatt
    10081 cgacaagacc ctcttgacac ttgtccgcgc cgtggtagta ttccgccgtt gacaggtacc
    10141 cggagacccg ctgaacgttc tgcggaaacc gggtgaaaag ggaagcgtgg taacccttct
    10201 cacggaacga gaagggaatt ccaccgcgga cgcgccgccg tgatgggcgg cgggcatcat
    10261 gattcgtggt taacagcccg ccggcgtccc gctcagtatg ccactgtcga gcctgcaacg
    10321 ggcctcgatg ggaaggcgag cgggaagcag ctctgagccg gaagacctgc ctgtcggagc
    10381 ctaagggtcc tcgcgttttt gggaccgcaa ggtcttgctc cctttatcac agatgatcga
    10441 gcagccttga cgtttcccga acgcggtggg ccacaatgtg tcctcgcgct cgggacacca
    10501 agggatcggc tgtcgctccc ctggacagcc gtaccgtgcc ttggccaccc ctcgaacacg
    10561 aggaatcaaa cgagtctgac atcgttcagg gggattcccc gcgcgcatcg gggagcagga
    10621 ggtaaagcat gtatcgtcgc aagctgttga ttacaggtat ggggttgatt gccgctgcct
    10681 ggggcgggcc gaccttggca ggtccttaca gcgggcccgc cgacaccgcc cacggggtcg
    10741 atcctgcgat ccccgccggc agcccgctct tcgtggagtg ggccgaccgg atccttccgc
    10801 tgggagcagg cacgtatttt gccccgcgtg ggagcaccag catttcgctc accggctaca
    10861 acagcctggg cgatctggac gccgcgcaga tcgccggtgg agactcgccc ggctatctca
    10921 cggtgacctt cccgacgggc atccgcgacg gtgccggtca cgacttcgcg gtgttcgaga
    10981 acggatttgc gtacggctcg ccgaatgggc tgttcgcaga gttcgcctat gtggaggttt
    11041 ccaccaacgg tgttgatttc gttcgcttcg acggcatttc aaccaacacg gctccggtcg
    11101 cgggctctgg cgcgttcgcc ggttatgacg tgtccaacgt atacaacttg gccggtaagc
    11161 acgccgccgg atacggcaca cctttcgatc tcgcggaact gatcaacaag ccgctggtac
    11221 tcagcggcca ggtcgatctc aataacatcc ggttcgtgaa gctcgtggac attcctggca
    11281 acggttcctt cttggacagc cttggcaatc cgattctcga taactggctg accacaggaa
    11341 ccggcggctt cgatttccga ttgccggccg gacaggggat aggcgtcatc aatgtcgcgc
    11401 ccgagccggc gtcgttggcc ctcatcggct tgacttgcgc attgttgacc gcgcgacgca
    11461 aatctcggcg agcatcgctg cgcttgtttg cgggtgtcgt gttgtgcatg ggattgctca
    11521 agaccggccc cgttcaggcg gtcacgttcg acgacatcga ttactggatc ggcaagggca
    11581 gtaatcgtgc cggtttcatc atcgactgga acgacggcac gtctgcggaa tcgctggtct
    11641 ggggctatcg ctgggatggc acgaagaagg gtgaggacat gctgcgggac attgtcgcgg
    11701 ccgacgctcg gctgttcgcg ctcgtcagcg gatcgggcgg ctacggcgcc gcgctctatg
    11761 gcatgggcta cgacctcgac aatgacggcg tgttcggagt ctcgcctgcc ttgagcttcg
    11821 acgccggcgg catggcgatg ggcagtccga atgactcgcg cgtgccgacc gatactgccg
    11881 accactggaa ggaaggctgg atgagcgccg gctactggag ctactggctc agcggtagcg
    11941 gggacagtcc gagctggggc ttcagcggta cgggtatgtc aggccgaaca ctgaccaaca
    12001 actgctggga tggctggagt tgggcccccg gcttctccgg aagcgcacca tctgaaccgg
    12061 tccccgcaca ggtccccgat cccgccagct catcactgtt cctgactgga tcactatgtg
    12121 tcctgcgtcg tagatgatga gaggaagcgc aaccgcgccg gcctcgctcc ttcgcggggc
    12181 gaggtcggcc tttgctgttg gaggagacac agatggcacg aacgattgtt acgtgtgcag
    12241 caacgatcct ggtagcgatg ggttccgttg ccgtggccta tgactacgtt tacagcccct
    12301 cggatttcgc cgttgaagtc gtcgagtacc agcaaggcgg cccggtccca gtggattgga
    12361 tctggaacac gccctttaac ctgcccgagg cttcacttgg gcgaccgact atagatacca
    12421 ctggcgactg gtggaccggt ccgccgaccg atccgattac cgtagttccg gtctacccgg
    12481 catttcgcgt ttgggagatg gtgtctatcg gtgaaggagg acgcttggtc cttgcgtttg
    12541 accatgcggt ggagaacgat ccgcggaatc cgtgcggaat cgacttcatc atcttcggca
    12601 acgcctttca ggtcatcaac ggtggctctt actggcgcaa cggtgacccg agccagtgca
    12661 tcatcggcac gtcaagcatg tgggccgagc ctggcacggt ctccgtggcc cagcactatg
    12721 atcccgcata tcccgagatg acggtctggt acacgtttga caacccgccc ggggccgaca
    12781 tgttcgcccc cacgctgggc cggatctacg accccgataa tgccccgtcc ggtttgcccc
    12841 acaacggctg gtggggagca ccgacgcacc cgctgattcc cttcaacccg aacctcggac
    12901 cggccgattt cgtgggtcac tcgctggccc actacgcgcg gaagtacggt tactgcgccg
    12961 gtggtacggg attcgacctc gacgacgtcg gcctccaatg gtttcggtac atccgcatcg
    13021 acaacccggt cggcagcggt atgacgcctg agatcgacgc ggtcgccgat gtcgatcccg
    13081 acgctccggc tccagacttc gactgtgata cggacgttga tgtggatgac atggccttct
    13141 ttgagtcttg cgccaccggt ccgggactcg ggccaccggc cgcgggatgc gagcggaccg
    13201 atctcgacca ggatggagac atcgatcagg cggacttcgc cgtcgtccag agatgctact
    13261 ccgggccgga tgtggcagcc gatccggact gcaaaggcct gtgagtcttc ggagaatcac
    13321 gatgaacggc cgtcagcgtg agcgacgcgg attcaccctg attgaggtgc tcgtcgtggc
    13381 ggcgctcatc gcgatgcttc tggcgattct gctgccctcg ttgggccgag ccaggcatct
    13441 cgctcgcagc gtacagtgcc tgagcaacct gcaccagatg ggcgtcgcgg cgcattccta
    13501 cgccaccacg cacggacgat ttccgccgta tctctacggc agtcccgacg gatcggtttc
    13561 gtatggttgg gacttgcggg tccactaccg atgggaaggc gggcagcgca aggccacggt
    13621 caagccgggc cttctctggc agggcaagac gatcgacgag atcaatcagt gtcccagcta
    13681 tcagggcggt gacaactggg tggactactc ccatacgggc tacaactaca acagcagcta
    13741 cgtggggtat tgcgaatact acgcggaggt cttggaatgg ccgcccgtcc caaccggcaa
    13801 gctcgtcgtc aaagagaacc cggctcgtcc cgaagacatc cgacgatcgg ccggctgcac
    13861 cttgttcggc gatggcgagt acgtcgccgg ggccaacaaa ttcatgcggt cgccgttcaa
    13921 gggccgcgac ggctcgtttt cgggcagatc ggccggcacc cagggatatc gccacctgaa
    13981 acgcacgaac gtggtgtttg ccgatggcca tgccgagtcg tggatcaagc gttttgccga
    14041 cacctatgcg ttcgaccagc ccaacgtgcg gccgcatggg gatgtcccaa ccgggtttct
    14101 gtcggccgac aacggcctat atgacttgga ctgacgtggg gccaggcggc atgaacctca
    14161 cgaaaacaaa tgacaacgcg acaaggcgga ggagtcctga tatgccacaa ggctacgttc
    14221 acgtatatac cggcaacggg aaaggcaaga ccactgccgc cctgggcttg gcgctcagag
    14281 ccgttggtgc cggccatcgg gtgtttatcg cccagttcgc caagggcatg gacagtagtg
    14341 agtcgcgcgc tttcgcacgc ttgtccgatc gggtggtgct gaggcagttc ggacttccgc
    14401 acttcgttcg gggacaagcg acgccggcgg acgtgacgat agcctcactc ggtctgcgtg
    14461 aggtggaatc cgcggttcac ggcggcgagt acgacatggt cgttctggac gaggcgaacg
    14521 ttgccgtgaa gcttggtctt ctcccattgg actggctgct ccagttgatc gttgagaaac
    14581 ccgaaccgtt ggagctggtc atcacgggtc aggcggctca tccgcgactc attgaaatcg
    14641 ccgatctggt caccgaaatg cgcgaggtca agcactattt ccgccgcggc gtcttggccc
    14701 gaatcggcgt cgacatgtga accatgcctg ccctccgcct ggcaggaaac cacgcgaggc
    14761 cttccacacg aagtgcgacg cgcgaatgaa cgtcggcgac gcctgtagcg agcgccggcg
    14821 accctccgat tcgttcgccg tgcgtgccgt gtatgatccg ggaaatccgc gcagccggtc
    14881 agggccgttt ccgtcgccgg ccctacttgg gcacttcgct cagcgggatg cccagggctt
    14941 tggcaacacc ctcgccgtag gcggggtcgg ccttcaggca gttcccgatg tggcggatct
    15001 tgatctcccg cggtgcatcg cccatcgccc gggccgtatt gccgaataga gcctcctgtt
    15061 gctgggggct catcagcctg aacagggcac cgggctggga gtaatagtcg tcatcgtcct
    15121 ggcggtggct ccagtggtcg gccgcgccct cgatggccag aggcggctcg cggaattccg
    15181 gctgctcctg ccactcgccg tagctgttgg gctcgtagcc cagagtgccg ccgtagttgc
    15241 cgtccacccg catcctgccg tcgcgatgga aacattggac cgggcaacgc gcgcggttga
    15301 ccgggatcag atgataattg acacccaggc gataacgctg ggcatcaccg taggagaaca
    15361 gccgcccctg caacatcttg tcaggcgaaa agccgatgcc gggcacgatg ctggccgggt
    15421 tgaacgccgc ctgctcaact tcggcgaagt agttctcggg gttcttgttc agctcgagta
    15481 cgcccacgtc gatgagcgga taatccttat gaggccagac cttggtcaga tcgaacggat
    15541 tgaacgggaa cttggcggcg ccttgctccg ggaccacctg gatcttcatg tcccacttgg
    15601 gaaaatcgcc cttctcaatg ctctcgaaca ggtcgcgctg atggctttcc cggcacttgc
    15661 cgatgatggc ctcggcctcg gcgtcggtga ggttcttgat tccctgccgg gtcttcaggt
    15721 gaaacttgac ccagaatcgc tcacccttgg cgttgatgag gctgaacgtg tggctgccgt
    15781 agccgttcat gtggcggtaa gaatacggaa tgccccgatc gctcatcgta atcgtgacct
    15841 ggtgcagggc ctcgggcagc gacgtccaga agtcccaatt gttgcgggcg ctgcgcagat
    15901 tggtcttggg atcgcgcttc acggcatggt tgaggtcggg aaacttcagc ggatctcgca
    15961 ggaaaaagac cggcgtgttg ttgcccacca ggtcccagtt gccctcctcg gtgtagaact
    16021 tgatggcgaa cccgcggatg tctcgctccg catccgccgc cccgcgctcc ccggccaccg
    16081 tggaaaaccg gacgaacacg tcggtcttct tgccgacctt ggagaagatc ttggctttgg
    16141 tgtagcgggt gatgtcgtgc gtgacggtga acgttccata agcccccgag cccttggcgt
    16201 gcatccgccg ctccggaata acctcgcggt cgaagtgagc gagcttctcc aggtaccaga
    16261 cgtcctgcaa caacatcggg ccgcgcggcc cggcggtcag aacgttttga ttgtcggcaa
    16321 ccggcgctcc gacctcgttg gtcagtttct tttcattcat agcgttctcc tttctgagtg
    16381 aagagcagtg caagactaag tcccgtttta gatatcatcc aaatcatgat gaaaaaaact
    16441 tacttcttct gcctgcggcc gcgaaggacg acctgatact cccgaactgt gaaccggcga
    16501 agcgattccg cacccgtcac gcgaagagcc tcccagggca cgtcgtagac cttgcccgtc
    16561 tgctcgtcga tgaagtggtg atgaggccgg acttcggcat cgaacagggc caatccttcc
    16621 ttgagcactt gctgcctcat taatcccttt ttcacgaaca ggttcaacgt gttgtagacc
    16681 gtggcccggg agatggtcgg acatcgcttg cgaaccttcg cccaagcctc ctcggccgaa
    16741 ggatggcttt cacggttgag cacgacccga gctaccgcaa gacgctgcgg cgtcgggtga
    16801 atgccgtgcc gacgaagaat ggcgatcaga tcctccacga tgattagact aggtctaagt
    16861 ccggactttg tcaagagccg ctgcgtttcc ggagggcact ggcaaacaag ccgctgacgg
    16921 catgggtgcc cgaaccgtca cggaaccggc cgcgccgacg accgcctgac cctctcggcc
    16981 tcggccgcgt tttcgttcgg acaaaccgct tatgggaagt ggtttgtgtt catggggcgg
    17041 ttgacccgct ctgccacttg ggggtgatcg cctcccggcc cgattcgacg ttgttgccgc
    17101 aggatcggat tctcgtccca atgggcgata tcaaaaagtc tttactcccc cggtttggat
    17161 acgcgctttg cgacgatgtc tcggatctgg gccacgttga atccatgccg gcgggacagt
    17221 cgtcctaccc gttcctggtc agaaacgtcc gagggcaacc caagatcaga cttcagcgta
    17281 tccgccgtca cccccgtggc ggcccggatg tttccgaggg acatggaacc gctgatttgt
    17341 ccccgcccct caccgttccc gcccccgcgc cgctctcggc cgacgtcgtc ccgcatgcgc
    17401 cccaggcggc tttccgcgtc gccccttgag atgacctgtt tcacggcgct gcccgcgtac
    17461 cacgccaaac ccggaaatgc gagcaacacg gcaaggagaa acacgatgcc acagacgttg
    17521 catccgccgg cacgaggcgg gtcggtttcg gttcctccga gcattcgctt gaccatcacg
    17581 cacacccacg accagtgcgg tgcaacgtga acgatcggca acatggcatc gggctcctat
    17641 tccaggggac cacgaattgg ctcttggcat ccgtctcgaa ttgatcgagc acgggcgagt
    17701 aggtccgaat ctgaatctcg ttgtcggccg gcacgaaacg gatgagccgc agccggccgt
    17761 tgccgccgtt ctcgcgctcc tggtagtcgg cgagcatttc gagcacgtcg gcacctgcgt
    17821 cgtttcgcga gatctggtga tactccccga cactgccaat gtggccgcag agaaccatga
    17881 aaacttgcgg gctgcggcgg atgagcttct gccacatctc ctcgcccgag ttgccatcct
    17941 tgcgacactc tcgctgcgtc tgccgaccga ccccggcgtc gcctcgcagg tagctgtgcg
    18001 tggtgatgat cgccgaccgg gtgggatgcc ggtcgagaac accccgcgcc cagtcgaggg
    18061 catcgtcggg cgcatccagt tcaagatgca ggatgacgaa gtcgaccccg cccccgaaga
    18121 aaagctggta cgaatttagg ccgttgggcg acgcgccgcc gtaccacggc cggcccttga
    18181 atcgccgctc ggggtcgaaa tagcgcagat acatcgtcgc gatgcccttg cgggcgcctt
    18241 cgtcgctgtc atagtcgtgg ttgccgatcg cgatgccgta aggaaccacc ccgtcgagga
    18301 cggccatggc ctcgtcggca accctccact gcgagggctc gtagccgtgg ttctggacga
    18361 gatcgcccac gtgtgtaacg aaaacgatgt tctccttgtc gcggttgagt ttgatccagt
    18421 tggtctgggc gaaaaacaga tccggccgtt tctcgctgta tatctgcgtg tccggaagca
    18481 tcacgatcga gaacggacgc cgggcgtcgg tttgggttgc tggtcccgtg cagccgacgc
    18541 ctgactgcag aatcagcagt acgctgaacc aaacgaaccc cgtgtaggcg gatgtcaggt
    18601 tgcggcacgg caagcgtacg acagttttca ttctgcctcc ttgcggtcca ttctggtgtt
    18661 ccacaacgac acgagcttca ggcgatcaga agacagaatc caggcgacat cgagtcggcg
    18721 ttgcctgttc ggctcgaccc gtcgggcgca tccgtcgctc ccagattgtt cggacgatcg
    18781 agatctggtc gctcggattc gctcgcgctt ctccgtcaag tcggtagggc cgcgtcgagg
    18841 tcgccgcggc gaccgagacc tgccatattg ttgagctcgg gccggcaggt cttcgccacc
    18901 tttgccggct ccgactatgc cctaccgtct tcacataagc tctcaaaagt tctgcaccgc
    18961 cctttccggc acgcatgcca gcgcctgctt cagatccttg acgagctttt cggccggttc
    19021 gatgccgatc gaaagccgta ccgtgttctt gcggacgccg aacatggttc gccgctcggg
    19081 cggcaggtaa agcatcgtag tcaaatacgg aatacagatc agtgactccg tcgacccgag
    19141 gctgaccgcg tgatagatgg tcttgagcgc cgcgacaaaa cgcttggcgt cctcttcatc
    19201 ttcgccgacg tcaaaagcca gcatcccgcc gaaccctcca tgcatctggc gcaccgcgac
    19261 ggcatgcccc gggtggctgg tgagaccagg gtacagaatg cgggtcacct tggggtgctg
    19321 cgccaggaac tcggcgacaa tctgggcccg ccgggaaatg gcctcggccc gcagctcaaa
    19381 cgttttgagc ccgcggtcca ggagaaacgc cgagtaggcg tccagggtgc tgccgatcgc
    19441 ctggcgggtg aaccacaacg tattgtagtg ctccttcgaa ccggcgatcg cgccggccat
    19501 gagatcgtta tgtcccccga gagccttggt ggcactgtga acgacaaggt ctgctcccaa
    19561 ttccagcgga tgctggtggt aaggagtggc gaaggtgttg tccacgacga gcatggcccc
    19621 gacggaatcg gccgcctggc ggaccgcgcc cacgtcgata atcttgcaca gcgggttgct
    19681 gggcgtttcc aggaagatca tctgtgtgtt gggccgaatg tgcttgccga tccggtcagc
    19741 ctcgtcggca tcgagccagg tgatctgcac acccatccgc tccaggatca acgacacctt
    19801 gtagttcgcc ccgtaggtgt cgtggaagat gatcaggtgg tcgccggttt tgagatacgt
    19861 cagatacacc atcgtacacg cggccatccc ggaggtgcag acgacgcagt cctcggcccc
    19921 ttcgagcgcg gcgatcttcc gttcgacctc gcgaaccgtc ggattgtcat cgcggtggta
    19981 ggtatagccg tcgatctcgc cgtcggtaat ctggcggagc acgtcgaaag agaggtattc
    20041 atagttcacg gcattgacga tgggcgtgac catcggccgg ttgccatacg gagtcaaggg
    20101 atcgggggcc attccgtcgt tttcctttcc ttctgaaaaa gcgtctggtc gttgcctgga
    20161 ccactacgcc cgtatcgtat cataaaccgc ttcatcctgc ctcaggattc gcgtgcgtgc
    20221 ggctgcccgg cctgcattgg ggaggtattg tcggtttcgg taggatcgcc aacggccaca
    20281 acgcccgcac catggcatat aggacactgc cgggcgagtg cgacgcgtat cgcatcacga
    20341 atgaacgcgg aacggttcgg gacgctcttc aacatgtccg ccaggcaagc gtccactcga
    20401 atggtgacca ctgtctcacg atgaggacgt cccatgatgc ggatcctcct gaagggtgag
    20461 cgtatcgaaa ccgtcccgga tggcatccca cagacgacgt atgtcctccg aagccccatg
    20521 atcggatgct tccacaacgg ctcgagcgtc gatctgtgcc tcggtgaatg cgcggtcgta
    20581 gcgaatccgg ccggccaccg ggatgaaatg ctcccgacaa tacgcttcga tgagttctga
    20641 catctcgggg ttcaggtccc acttgttgac gcacacggtc gcaacgatgc cgaaatggcg
    20701 ggtcaactgg atgacccgtc tcaagtcgtg ctcgccgctc agcgtcggct ccgtgaccac
    20761 cagcacaagc cccgccccgg tgatcgacgc aatgaccggg cagcctaacc ccggtgcacc
    20821 atcgatgata atcaactcga tgctccgctc ctcggcgata cggcgggcat taccgcgaac
    20881 gatactgacg agcttcccgg agttctcgga ggcgacgccc agcctggcgt gcaccatcgg
    20941 gccgcaacgg gtctcggaca ccatccactc gccgcacaaa cgctccggaa agtcaatcgc
    21001 gcgcaccggg cagacctgga cgcagacacc gcatccttcg caggcgatcg ggtcaaccgc
    21061 cagcgcgcct tgccgcgttt cacctatcgc ttcgctcata gcctgaatca tgtcgttgtc
    21121 tcttgcgggg caggatcttt cgcagtaagc gcaggaatga caatcatagg acgtgctccc
    21181 gacaatcact cccgagcttc cctccgggaa ccgccgtacc acggcgccga atctgcaatg
    21241 cgccaggcac atcccacaac tggtgcagtc cgcctgacga atgactgcct gatgaccgct
    21301 gtagaagtca tggcactcct tgacctgcgg tgagagaaca agatgaagat cggcggcatc
    21361 gacgtcacag tcagccacca cgcatcggcc ggccaggacg gccagcgagg cggcaatcga
    21421 cgtcttgcct gttccgccct tgccgctgat gacgaccagt tctctcatcg cgaaccccct
    21481 ccgctcatca cggcatgagg ccgcggagag gtcgccagtg tcgtcacttc gcgaagcagg
    21541 cttctgaagc gatcccggta ctcttgcgaa agcaacgcgg ccaattgtcc tcgcgaatac
    21601 gcctcggcga ctcgccgatc atctggtatc tccgctagaa ccggaatctg attcccgctg
    21661 caatactccc acgtggcccg gttaccggca tccgctcggt tcaccacaac gcccggcggg
    21721 agattcagcg tcctgaccat ctcaacggcc agtttgagat cgtgcagccc gaagggtgtc
    21781 ggctcggtta ccagcacgac gcagtcagcg ccgcgaac
//
LOCUS       DAOTPW010000081        21544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2509_length_21544_cov_5.784867, whole genome shotgun sequence.
ACCESSION   DAOTPW010000081 DAOTPW010000000
VERSION     DAOTPW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21544
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2509_length_21544_cov_5.784867"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="P5316_09180"
     CDS             complement(<1..883)
                     /locus_tag="P5316_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRR85171.1"
                     /translation="MDNDTQELTNRQLLKWMFGFLRPVKGIVALACLFLALWTGAEIA
                     AVRVTALAIDQIKTIHLSETRLVDPWEWLSGNYAEAAGLRHVVLALAAFTATVGVLGF
                     LRETTNARLSMHMVFYIREAVYDKLQRAGFGFHDAISTGELINRSLSDLQNVRSFINS
                     AVLLTLEIVLIVGGYIILLLSRSPWVAALALAPLPVWTVYIMRFSRRAQPALKAAMES
                     GDRNISILTENIAGVHVVKAFATEPHEIAKYAANCDDFFHKVMHRIRLFADFLPVMRS
                     IATASHLSLFLAAGILII"
     gene            complement(1310..2755)
                     /locus_tag="P5316_09185"
     CDS             complement(1310..2755)
                     /locus_tag="P5316_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85172.1"
                     /translation="MAHELGRREFLQASVATGAICFGLSQFASAQTAAGTPVLVSPGC
                     RGTKVKIARLYLVGTNKLWPTPKLDVNAERKRYEAEFDRMKDDFADIDFAVDEIIESA
                     ADVNKLGDRLQGVDGVLVIHMSMGTTDSLKQILAAKKPTVLFALPYSGHEWTGFGALR
                     RQPEGALLECMLTADMNELAAAVRPFRAIHHLREARILNITARKDQAAYNDSIKKKFG
                     TETVIIDRDRVLKTYESIPEADAQAEAERWMKQAQKVVEPSKEEIVRSCRLALTFEKL
                     LNEEKATVITADCYGSMYHQLPAFPCVGFVRLNNMGLGGICESDMAAAVTHIILQGLC
                     GKPAFISDPTLDTSTNSIILAHCLGSIKMDGPKGEAAPYRLRTIMERQEGCVPQVFMR
                     KGKVTQARLIGTDLMLYFTGDVTDAPDDERGCRTKITVKVDGDAEKLWENWSDGLHRS
                     TVYGNLTKDLRRFCRFTGIRLVNEAEELPNA"
     gene            complement(3383..4600)
                     /gene="trpB"
                     /locus_tag="P5316_09190"
     CDS             complement(3383..4600)
                     /gene="trpB"
                     /locus_tag="P5316_09190"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRR85173.1"
                     /translation="MTTTQSNLTDVPDAHGRFGPYGGQYVPETLMGAIHQLEEEYDRA
                     RKDPAFWAELEGYLKHYVGRPSPLYFARRLTDRLGGARIYFKREDLNHTGSHKINNTL
                     GQALLAVRMGKKRVIAETGAGMHGVATATAAAVFGLECVVYMGAEDVRRQKLNVFRME
                     LLGTHVVSVNSGQKTLKDAINEAMRDWMSSVQNTHYIIGSVMGPHPFPMIVRDFQAIV
                     GRETRQQFLEREGKLPDMIVACVGGGSNAAGIFYPFIEDKSVRLVGVEAGGEGLNTDR
                     HSATLCKGSPGVLHGMYTYVLQDEDGQTLPVHSVSAGLDYPGVGPVHSQWKDAGRADY
                     VAVTDQEALDAFTMLSETEGMIPALESAHAVAHVINIAKSFPRDFSIVINLSGRGDKD
                     CVEVARLLGKQIG"
     gene            complement(4764..5504)
                     /locus_tag="P5316_09195"
     CDS             complement(4764..5504)
                     /locus_tag="P5316_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRR85174.1"
                     /translation="MYTLLPLKVGECHTEQGPKMFFVRDFDKTYSTYFYFWYIEGAGT
                     RILLDTGFDRADSKGFMPTMIQQPGWTPAERLKQIRVDPRSIEHVIVSHLHFDHLSNS
                     VDLFPNARLYLQKREYETALYPPHPWFTAAYAPGMIDRLTGDLSRRLEIIDGDAEILP
                     GLKLILVGGHTPGQQSVILPTKLGPRTCLTSDICFFYKHIEEDWPIGFFCNLAEVFQA
                     MARFRREADVIIPQHDARLEKEFPMPKP"
     gene            complement(5579..6664)
                     /locus_tag="P5316_09200"
     CDS             complement(5579..6664)
                     /locus_tag="P5316_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR85175.1"
                     /translation="MIRPVWISTFAVFLPAMVGCAPKPKPVVATPIANVWESPPPPLP
                     APPKLEPLPDLESQPADQLPDTAEPIAVVNGEPLSRSALVNRLIESHGLAQLEQMILL
                     TVARQKASSLGLEVTPADVRAMHEESLKRLATPIVEPDARVLDAREAQRLLDEFLAAK
                     NISRREWDTRMEQKAYLRKIAEAEVAKTTISEDMLRNEYDQDYGEKVQVRHIQVGSLA
                     AATRVRALLAANKDFELIARQMSENPVTAAQGGLARPFTRNDPGVTPLLREAAFKLKP
                     GEVSEAIRDGNVYHIIRLERRFPASDVGFENADRAALRRKLIDRLIQQREQDLEAELF
                     QAATVDVRNEELARQYRERHRTGRSGP"
     gene            6874..7995
                     /gene="ispG"
                     /locus_tag="P5316_09205"
                     /gene_synonym="gcpE"
     CDS             6874..7995
                     /gene="ispG"
                     /locus_tag="P5316_09205"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRR85176.1"
                     /translation="MSSDPIPSRARRMTRQVRVGKVPLGGGAPVVLQSMTSTLTHDID
                     ATVMQINRLAEAGCDVVRVAVPTKEDTAALPAIIRQSPVPIVADVHFHYERALEAIEA
                     GAAKIRLNPGNIKDRKQVNEVIRASKQAGIPIRVGVNEGSIVERQDKAVRQLEKQELA
                     RDYHGNLVQLMVRKLEDYLRIFEENGFENVVLAAKSPDPTIVIDAYREISRRFDFPLH
                     LGVTHAGPPETGRIRSVAALATLLAEGIGDTIRISYAADPIHEVEDGKELLCCLGLRR
                     RTGPELIACPTCGRVEVDLLALVREVRERLASIKAPIRVAVMGCIVNGPGEAEGADVA
                     LLAGKGKAVICVRGEPVRTVPESEMLDALYEAVMSLDAP"
     gene            8131..8547
                     /locus_tag="P5316_09210"
     CDS             8131..8547
                     /locus_tag="P5316_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85177.1"
                     /translation="MTEDYVSAAARHLRDADTLLVAKAWDNAAYLAGYIGECGVKAVI
                     QHAGLSLRRIHLNEVKPEHLLLAADLSFAARRYPIDLDREAAELWRRWSVALRYARTG
                     EVDEPACRTLLDRAKLVFRKTVVQMVLDGVLQTVPK"
     gene            8544..9854
                     /locus_tag="P5316_09215"
     CDS             8544..9854
                     /locus_tag="P5316_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR85178.1"
                     /translation="MIHFEDVLPTAFKTIKNHRSHGLIEEVLVLRDLRGRVRLFLKAR
                     ENSADELASSIADLATSLETSLAPFWGGVLDVDRPKSEFYAILEQVRSDRQPIEPQSE
                     FPCWWIVERCAAKSAWMSREHKPPWPLNAHTPAIMAFYSHKGGVGRTTCLCAAAVNLA
                     RGAKKVAVVDLDLEAPGLGTLLADNPVEHGVADYLLEHLVAGSSFDPDIKDYVVKQSD
                     KDLIGTAGEPIVCVPVGRVDDHYVEKLSRIDYELVASMTNPGQSPLADLLLQLKQEFD
                     PEYVFLDCRAGLHDLGGLAVHCLSHAAVLLGLDSSQSWQGVRCIIRSLGLIDGPPRPL
                     LMVQAMDSYRSDDRAKDARARFLQQSYDAFTEHFYNEGDVPDIAGEDEPHYPFRIPYS
                     EALMGYQKLTEVVPLITNSPYVEFVAKIEEMASKGRNDDVREDS"
     gene            9835..11331
                     /locus_tag="P5316_09220"
     CDS             9835..11331
                     /locus_tag="P5316_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85179.1"
                     /translation="MSAKTVDDNLKWHLLEDLSTFARSGVDEVSGGEDFFKTHLPIPE
                     HRRVLDRDTLLVLGGRGTGKTRLFRALTQVQDPSDLLPGQSGSSQVRSDKYVKAFTLD
                     GTTFPRPEVLQKDFVARGQDDDQRLQSFWLGLLAGALATEPLTQAAIKESLPPAVYQV
                     LCDSLPDPNRWMDLVNQDIGGISSSLDRANDLLRAQSRNVVLVYDDLDRLVSQVSGVY
                     PLVRQLLTFWLNNFRRWSSIRCKVFLRTDIFEADEVAFTDSSKYRPLSVTLQWTADNL
                     YRLVLKRLVNGAKQEDWLVFVRGKLSRREDFRKSERWGLIPMTSESQHRSFMELLVGK
                     WMGSDKRRGDTYRWFLNHLQDSLGNIAPRSFLKLFEFSASRQLRKDLPGAGHLLAPEE
                     ISGALTEVSKDRLQELREEYDWIDDIGASLKGKTVPMERGEFRRCLGKVPWQKLPDFL
                     GEDKDRLVQYLISLGILRETEDKRIHVPDIYLFGLELRRKGGIRRPRV"
     gene            complement(11494..13059)
                     /locus_tag="P5316_09225"
     CDS             complement(11494..13059)
                     /locus_tag="P5316_09225"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRR85180.1"
                     /translation="MSQTTAEAAVEADPAELEQQIRARVESLSHLPTTVAVAMKLVEL
                     RDNPDAEPSDYAKVIGADASLSAKLLALANSPWFGIRNKITTVRNAINLLGLGTVRTM
                     AISYCMAGLHNELRLSRDESRRFWETCLCKAVAARCCAQKLNPALAEEAFVAGLFEDL
                     AVPVMYSVARQQYLAILADPACDAQGQLQREHDLFHLDHTGLGRILAQKLELPEVFVD
                     AIAFHHDQERLKAFMADMATAQAIHIAALFPHDLDAWNSRDANELCGIIDETEGLNEI
                     GPAAFLQEVQNGVDEYYRFFDEGGRLDKQLSDLLIQTSKEGAENTEHLVKTVHRLMQE
                     AANVGLQMNQLIDRNITLMDKANRDQLTDLLNREGFAAAAEQTLAKASRYGMSFAVAF
                     FDVDKFKTFNDAYGHHFGDRVLRSIAQCLTTSTREQDIKARMGGDEFILLIPDCSKAE
                     AQDTVKRLLEQVAAATIGRHTDQARATLSAGLLYVTPSHTQHPLQSLIDAADKLMYES
                     KQAGGNRVTSRVI"
     gene            complement(13526..14917)
                     /locus_tag="P5316_09230"
     CDS             complement(13526..14917)
                     /locus_tag="P5316_09230"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRR85181.1"
                     /translation="MDRIVALCKRRGFIVQSSEIYGGINGFWDYGPLGVELKRNIKEA
                     WWKDMVQDREDMVGLDCSIIMHPQVWKVSGHYDLFSDMMVDCKACKARFRADQLKEMQ
                     CPMKPSKHPGEHDKCELTEPREFNLMLETYPGPIKDEANRAFLRPETAQGIFVNFKNV
                     CDSTRIKIPFGIAQIGKSFRNEITPRNFTFRSREFEQMEIEFFCHPSESNKWYEYWRD
                     VRFNWYTSLGLAGNKLRLREHGKEELSHYSCGTSDIEYAFPFLAEGDFGELEGVAHRG
                     DFDLRSHMEGKLINKDGKLVLDVGPDGKPRWKGSGKDLSYYDDERKERFIPHVIEPSA
                     GADRGTLAFICEAYTVDESRPSPELMKFHPRLAPIKAAIFPLVNKDGLPEIAEPIYRE
                     VRKRWPAQYDAKASIGKRYARMDEAGTPYCFTIDGQTKEDGTVTVRDRDTGAQQRINK
                     SRVTEYLTEKLSR"
     gene            15381..15632
                     /locus_tag="P5316_09235"
     CDS             15381..15632
                     /locus_tag="P5316_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HRR85182.1"
                     /translation="MVRMMIELPEGVLAALRKDPKSFAEEMRLAAAVKWYELGLVSQG
                     RAAEIAGLPRRQFIDALSRLSVSPFQYAPDEVVREAEGE"
     gene            15625..16107
                     /locus_tag="P5316_09240"
     CDS             15625..16107
                     /locus_tag="P5316_09240"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HRR85183.1"
                     /translation="MSETWVVNASPVIALARIGQLRLLEELASPLLLPEAVAAEILAG
                     PDDDPARAAVLSGWASRRSPAQVPDAVTEWGLGAGESAVLAIALDLRAIAVLDDATAR
                     AAARALGVALIGTLGVVVRAKQRGLVPLASSVFADLRAAGLYLDDDVVRVTLERIGEK
                     "
     gene            complement(16261..16878)
                     /locus_tag="P5316_09245"
     CDS             complement(16261..16878)
                     /locus_tag="P5316_09245"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRR85184.1"
                     /translation="MKTPERHSDTTPSPDEASIADGRPIVCIFGSYSPKPGEPLYEQA
                     YAIGHALAKAGFVVANGGYDGIMEASAKGAKDAGGSTIGVTCDIFSDYRGRPLKANRY
                     IDREISHKTVLARIEQMIEMSAGYVILEGGTGTLSEFGIVWEYVAKKLIRPRPIFIVG
                     DFWKPTVERVTAARPSHGKHVHCVSTPEEIIAIARAAIPIPRTGS"
     gene            complement(17271..17762)
                     /locus_tag="P5316_09250"
     CDS             complement(17271..17762)
                     /locus_tag="P5316_09250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR85185.1"
                     /translation="MKQATFHTEFGTMTARFFPDVAPKHVENFLALAEEGFYDNRTFH
                     RIVEGFMIQGGCPRGDGTGNGPRRLRAEFNDKPHQLGTLSMARTNDPDSASCQFFICL
                     DRCEFLDGKYTVFGELADESSIQTLKKIGAVKTRDPGTGEKSFPVKPVYIQKITVQEV
                     PDA"
     gene            18055..19053
                     /locus_tag="P5316_09255"
     CDS             18055..19053
                     /locus_tag="P5316_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRR85186.1"
                     /translation="MEPYPLLFEPIYKPKLWGGRRIFDHFNREPVGSEPIGESWELVD
                     LEGDASRARNGPAKGRTLTELVKRWGRALLGNADLFEGRFPLLIKYLDARENLSIQVH
                     PTEQVARELGGNVRVKHEAWYVLDADPAGVIYHGLEPGVSRDVFVEAMRTGLVEGVLR
                     KISVSQGDCYYLPSGTPHALGAGVLVAEVQTPSDITYRTYDWGRVDPASGKPRELHLE
                     QAVRCIDFTSPPASPMQQHLHLGTTMATVTRLVGCPCFVMDKLDIAQGVTQDAPMESF
                     AIWVLLKGLGRVEWGSGEERLDCRPGDVVLVPAGLGEVRARAITPAQWLEVTVPAR"
     gene            19404..19700
                     /gene="groES"
                     /locus_tag="P5316_09260"
     CDS             19404..19700
                     /gene="groES"
                     /locus_tag="P5316_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRR85187.1"
                     /translation="MAKLKVRPLGDKVLVKRVEAESKTAGGIVLPDTAKEKPKRGIVQ
                     AVGDGKLLENGERSKMQVNKGEEVLFSSYAGTEIKIDGEEFLIIEESEILAVLS"
     gene            19777..21411
                     /gene="groL"
                     /locus_tag="P5316_09265"
     CDS             19777..21411
                     /gene="groL"
                     /locus_tag="P5316_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982512.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRR85188.1"
                     /translation="MAAKRIAYDMEAREAIRRGVKQLAKAVKVTLGPCGRNVVLEKSF
                     GSPTVTKDGVTVAKEIELEDAYENMGAQMVKEVASKTSQVAGDGTTTATIYAEAIYDE
                     GLKNVAAGADAMELKRGIEQAVEAVVEELGKMATDVKNSEQVAQVGTCAANQNAEIGQ
                     QIAKAMEKVGKDGVITVEEGKTLETTVELVEGMQFDKGYLSPHFVNNYETMEVVLEKP
                     YILIHEKKISAVKDLIPLLEKVARAGRPLLVIAEEVEGEALATLVVNKLRGTLQCAAV
                     KAPGFGDRRKAMLEDIAILTGGKALFEDLGIKLESVDLKDLGQAKKVTIDKDNTTIIE
                     GAGSSSAISGRIQQLKNEIEVTTSDYDREKLEERLAKLAGGVAQINVGAATEAEMKEK
                     KALVEDAMHACRAAVEEGILPGGGVAPLRTLGALDKLAKKLKGDQRTGVDIVRRALMA
                     PIRQIAENAGLDGSIVCQKVMESEDKNFGYNALTGEYGDMVKMGVLVPRKVERVALQN
                     AASIAALLLTTDAAVVEIKEEKKKSGGPGGHGHGPY"
BASE COUNT         3910 a         5853 c         7230 g         4551 t
ORIGIN      
        1 gaatgatcag gatgcccgcg gcgaggaaga gcgagaggtg cgaggcggtc gcgatgctgc
       61 gcatgaccgg gaggaagtca gcaaacaggc ggattcgatg catgaccttg tggaagaagt
      121 catcgcagtt ggcggcgtac ttggcgatct cgtggggctc ggtggcgaac gccttgacca
      181 cgtggacgcc ggcgatgttc tcggtcagga tggagatgtt ccggtcgccg gactccatgg
      241 ccgccttgag agcgggctga gccctgcggc tgaaccgcat gatgtacacg gtccagaccg
      301 gcaggggggc caaagccagg gccgcgaccc agggcgagcg cgacaacaag aggatgatgt
      361 agccgccaac gatcagaacg atctcgagcg tgagcaagac agccgaattg atgaacgagc
      421 ggacgttctg gaggtcgctg agggagcggt tgatcagctc gccggtggag atggcgtcat
      481 ggaagccgaa gccggctctc tggagcttgt cgtaaacggc ctcccgaatg tagaagacca
      541 tgtgcatact gagtctggca ttggtcgtct ctcgcaggaa gccaagcacg ccgaccgtgg
      601 cggtgaaggc tgccagtgcc aacacgacgt gccgaagccc ggcggcctca gcatagttgc
      661 cgctgagcca ttcccacgga tccacgaggc gtgtttcgct gagatggatg gttttgatct
      721 ggtcgatggc cagggcggtg acgcggacgg cggcgatctc ggctccggtc cacaaggcca
      781 agaacaggca ggccagtgcg acgattcctt tgacgggccg gaggaatccg aacatccact
      841 tgagcaattg tcggttggtt agttcttggg tgtcgttgtc cactggaagc catcgggtca
      901 gcgggccagg ccgacggccg tgtccacagt cacgcgcgtt ggtcgaagca ctccgcggcc
      961 gccctgagtt tggcgggggt gcagtttacc acatccgtcg cggggattcc atggtctcgc
     1021 atcggcaggt cctgcggtaa gtcgtttttt ggcaagggct tgtattttgc acaagggccg
     1081 ttgtcagggg caagccgctc gctgcgcagc gggatctttc ccggcggcgg ttccaggagt
     1141 tgccgaggac cggggctctt gtatcaggag cccgttttgg ggggcgaaga gccggggtgc
     1201 gagattggaa ccttacggcc agacatctgc gcggcttgcg cgcatgtttc tggatcacag
     1261 agcgcttggg ttgcggcccg caaaaaggcg gtccgccttg ggaggccgct tatgcgtttg
     1321 gcaattcttc ggcctcgttg accaagcgga tgccggtgaa acggcagaac cgccggagat
     1381 cctttgtcag gttgccgtag acggtggagc ggtgcaggcc gtcggaccag ttttcccaga
     1441 gcttttccgc gtcaccgtcg actttcaccg tgatcttggt gcggcaaccg cgttcgtcgt
     1501 caggtgcgtc ggtcacgtcg ccagtgaagt acaacatcag gtcggtgccg atcaggcggg
     1561 cctgggtgac cttgcctttg cgcatgaaga cctgcgggac acaaccctcc tgccgttcca
     1621 tgatggtgcg gaggcggtag ggtgccgcct cgccttttgg gccgtccatt ttaatcgagc
     1681 cgaggcagtg ggccaggatg atcgaattgg tcgaggtgtc caaggtgggg tcgctgatga
     1741 aagcaggttt gccgcagagg ccctgaagga tgatgtgggt cacagccgca gccatgtcgg
     1801 actcgcagat gccgccgagg cccatgttgt tgagccgcac gaatcccacg catggaaacg
     1861 cgggaagctg gtggtacatg ctgccgtagc aatcggcggt aatgacggtg gccttctcct
     1921 cgttcaagag tttctcgaac gtcagggcca ggcggcagga gcgaacgatt tcttccttgg
     1981 acggctcgac gaccttttgg gcctgtttca tccaccgctc ggcctcggcc tgggcgtcgg
     2041 cctcggggat tgattcatag gttttgagga cgcggtcgcg gtcgatgatc acggtctcgg
     2101 tgccgaactt cttcttgatc gagtcgttat aggcggcctg gtccttacga gcggtgatgt
     2161 tgaggattct cgcctcgcgc aggtggtgga tggcccggaa cggccgcacg gcggcggcga
     2221 gttcattcat gtcggccgtg agcatgcact cgagcaaggc gccctcgggt tgtcggcgca
     2281 aggcaccgaa gcccgtccat tcgtggccgg agtaggggag ggcgaacaag acggtcggtt
     2341 tcttggcggc cagtatctgt ttgaggctgt cggtcgttcc catactcatg tggatgacga
     2401 ggacgccgtc aacaccctgg agccgatcgc cgagcttatt gacgtcggcg gcggattcga
     2461 tgatctcatc gacggcgaaa tcaatgtcgg cgaaatcgtc tttcatccgg tcgaactcgg
     2521 cctcatagcg ttttcgctcg gcgttgacgt caagcttggg cgtcggccag agcttgttgg
     2581 tgccgaccag atagagccgg gcgatcttga ctttcgttcc gcggcaaccc gggctgacca
     2641 gaacgggggt gccggcggcg gtctgcgcgg acgcgaattg tgacagaccg aagcagatgg
     2701 ctcctgtggc gacggaggcc tgcaagaatt cccgacgacc gagttcatgt gccatgatgg
     2761 actcctcctg ttgaatcgat ttgagtcata cagacatggt cagcctatca gatcggggct
     2821 tgcctttcca gcgattgcag atttcagatt gcacatttca aatccgagga actcgatcga
     2881 caaagggcgt agatcgccgg gcgcatccct gcaaaacgcc ccgatgaagg agtgtggtgc
     2941 gagttcgcag gcacgaatca gcgtgccccg gaggggcata ggtatgtagt caggggcgca
     3001 agcccctggt cttcgatgcc acacagaccg tcaagccccc agcggggcga cggattcatg
     3061 tcggcggatc agggtttcag ttcccgacgt gccgttcttc attcgcctcc aatgattccg
     3121 ccctcgtggt cgtagagacg ctcacggagg tcgggggtga tccggggcag ccggcgcttc
     3181 ttgcggaaga acaaggtggt aatgcagaca ggtgagggtt tgtctcatga ccgtgtcttc
     3241 tttccgtcgc cccttcgggg ctggatggat accgggtgca tttgtaccag cggcttgcgc
     3301 ccctgggtac atacttcttc ggggcatgaa acccgccggc tcgttttacg gaccgctgat
     3361 tgctgaaagg taaccgcttc ttctatccaa tctgcttgcc cagcaggcgg gccacctcga
     3421 cgcaatcctt gtccccgcgg ccggagaggt tgatcacgat gctgaaatcg cgggggaagc
     3481 tcttggcgat gttgatcacg tgggcgacgg cgtgggcgct ctccagtgcg gggatcatgc
     3541 cttcggtttc gctcagcatg gtaaaggcgt ccaaggcttc ctggtcagtg acggcgacat
     3601 agtcggcgcg gccggcgtct ttccattgcg agtggaccgg tccaacgccg gggtagtcaa
     3661 gcccggccga gaccgagtgt acgggcagcg tctggccgtc ttcatcctga aggacatagg
     3721 tgtacatgcc atggagcaca ccgggtgaac ctttgcagag ggtggcggaa tgccggtcgg
     3781 tattgagtcc ttcgccgccg gcctcgacgc cgacgagtcg cacggactta tcttcgatga
     3841 agggatagaa gattccggcc gcgttgctcc cgccgccgac gcaggcgacg atcatatcgg
     3901 gcagcttgcc ttcgcgttcg aggaactgct gccgtgtttc gcgtccgaca atggcctgga
     3961 agtcgcgaac gatcattggg aacgggtggg ggcccatcac gctgccgatg atgtagtgcg
     4021 tgttctggac gctgctcatc caatcgcgca tggcctcgtt gatcgcgtcc ttgagggtct
     4081 tctggccgct gttgaccgag acgacatggg tgccgagcag ttccatacgg aacacattga
     4141 gtttctggcg gcggacgtct tcggcgccca tgtagacgac gcactccagg ccgaagacag
     4201 cggcggctgt ggcggtggct acgccgtgca tgccggcgcc ggtctcggcg atgacgcgtt
     4261 tcttgcccat gcgtacggcc agaagggcct ggccgagggt gttgttgatc ttgtgcgagc
     4321 cggtgtggtt caggtcctcg cgcttgaagt agattcgtgc gccgccgagg cggtcggtca
     4381 agcggcgggc gaagtagagt gggctgggcc ggccgacata gtgcttgaga tagccctcca
     4441 actcggccca gaaggcgggg tccttgcggg cccgatcgta ttcttcctca agttggtgaa
     4501 tcgcgcccat gagcgtttcg gggacatatt gtccgccgta ggggccgaac cggccgtggg
     4561 cgtcgggaac atcggtcaga ttcgactgcg tcgtcgtcat ttgggctccg atccgagggt
     4621 tcaaaaccat ctggcaagat tataaccggg gggcgggaaa tgacgaatgt cgaaatccgg
     4681 atgacgaagg ccgaagtgga ggcagagtgg ctcccggctt gattccgcct tccgccttca
     4741 tcctttcccc tgaaccctga accctatggt ttcggcatcg gaaactcttt ttccagccgg
     4801 gcatcatgct gcgggatgat gacgtccgct tcacggcgga accgggccat ggcctgaaag
     4861 acctcggcca ggttgcagaa gaagccgatg ggccagtctt cctcgatgtg cttgtagaag
     4921 aagcagatgt cgctggtgag gcaggttcgc gggccgagct tagtgggcag gatgacggat
     4981 tgctggccgg gggtgtggcc gccgacgagg atgagcttca gaccgggcag gatctcggcg
     5041 tcgccgtcga tgatttccag ccgcctgctc aggtcgccgg tcagtcggtc gatcatgccg
     5101 ggcgcgtagg cggccgtgaa ccacgggtgg ggcgggtaga gggccgtctc gtattctctc
     5161 ttctgcaagt acaagcgggc attggggaac aggtccaccg agttggacag gtggtcgaaa
     5221 tgcaggtggc tgacgatgac gtgttcgatg gagcgcgggt ccacgcggat ttgtttgagc
     5281 cgttcggccg gggtccagcc gggttgctgg atcatcgtgg gcatgaagcc cttgctgtcg
     5341 gcccgatcga agcccgtgtc gagcaggatc cgcgtgcctg ctccttcaat gtaccagaaa
     5401 tagaagtagg tggagtacgt cttgtcgaag tcgcgaacaa agaacatctt cgggccctgt
     5461 tcggtgtgac actcgccgac cttgagggga agtagcgtgt acatgcccga ctcccgtgtg
     5521 agttgtcggt tctcaggttt cagttctcag ttttctgtca ctgaccactg atgactgatc
     5581 acggaccact tctccccgtt cggtgcctct ctcgatactg ccgggcgagc tcttcgttgc
     5641 gaacgtcgac ggtggcggcc tgaaagagct ctgcttcgag gtcctgttcc cgctgctgga
     5701 tcagcctgtc gatcaacttg cggcgcaggg cggcgcggtc ggcattttcg aagccgacgt
     5761 cgctggccgg gaatcgccgc tcaagccgga taatgtggta gacattgccg tctcggatcg
     5821 cctcggacac ctcgccgggc ttgagtttga aggccgcttc ccgcaacagg ggggtgacgc
     5881 cggggtcatt gcgggtgaac gggcgcgcca agccgccttg agcggcggtg accgggtttt
     5941 cgctcatctg cctggcgatc agctcgaagt ccttgttcgc cgcgagcagg gctctcactc
     6001 gcgttgcggc ggcgagggat cccacctgga tgtgcctgac ctggaccttc tcgccgtagt
     6061 cctggtcgta ttcgtttcgg agcatgtctt cggagatggt ggttttggcg acctcggcct
     6121 cagcgatttt tcgcaggtat gctttttgct ccatgcgagt gtcccactcg cggcgggaga
     6181 tgtttttggc tgcgaggaac tcgtcgagca gtcgttgggc ttcgcgggca tcgagcactc
     6241 gggcatccgg ttcgacgatc ggagtagcga ggcgtttcaa agattcctcg tgcatggccc
     6301 tgacgtcggc cggtgtgacc tccaggccga gggaggaggc cttctgcctg gcaacggtca
     6361 gcaagatcat ctgctcaagt tgggcgaggc cgtggctttc gatcagtcgg tttacgagcg
     6421 ctgaccgcga gaggggctct ccgttgacca ctgcgatcgg ctcggcggtg tcgggcaact
     6481 ggtcggcggg ttggctttca aggtccggca ggggctccaa tttcggcggt gccggcaggg
     6541 ggggaggggg ggattcccag acgttggcaa tcggcgtagc gacgaccggc ttgggtttgg
     6601 gcgcacaccc aaccatcgca ggcagaaaca cggcgaatgt gctgatccag acggggcgaa
     6661 tcatcacggt cctttcggag cgcatgtgaa cacaaacggc agcttggatt ggcaggtctt
     6721 tgcggcctcc ggcagcatcg accatgcccc acttggaggg gaagctatcg gttggcggtc
     6781 tgccggtcaa gacgggcggc ccgacctttt tgacggtgtt ggccagtggt tctagaatcg
     6841 gccgttggtc agccggagca ttgtgaggca ggaatgagca gcgatccgat tccatcccgt
     6901 gcgcgccgca tgacgcgaca ggtccgcgtg ggcaaggttc cactcggcgg gggggctccg
     6961 gtcgtccttc agtcgatgac cagtacgctg actcatgata tcgacgcgac agtcatgcag
     7021 atcaatcgct tggcggaggc cggctgcgat gtggtacggg tggccgttcc gacgaaagaa
     7081 gacacggccg cgttgccggc gatcatccgc cagtcgccgg ttccgatcgt ggcagatgtg
     7141 cattttcatt atgagcgggc attggaggcg attgaggcgg gggcggcaaa gatccggctt
     7201 aatccaggga acatcaagga ccgcaagcag gtcaacgagg tgattcgggc cagcaagcag
     7261 gcgggcattc ctattcgcgt gggggtgaac gagggcagca tcgtcgagcg tcaggacaag
     7321 gcggtccggc agttggagaa acaggaactt gcccgtgatt atcacgggaa tctcgtccaa
     7381 ctgatggtcc gcaagttgga ggattacctg cgcatcttcg aggagaacgg gttcgagaac
     7441 gttgttttgg cggccaagag ccccgatccc acgattgtga tcgacgctta tcgcgagatc
     7501 agccggcggt ttgattttcc gctgcacctg ggtgtgacgc acgccgggcc gccggagacc
     7561 gggaggatcc ggtccgtggc ggcgctggcg acactgctgg ccgaggggat cggcgacacc
     7621 atacgcattt cgtatgccgc cgaccccatc cacgaggtcg aagacggcaa ggaattgctc
     7681 tgctgcctgg gcctgcgtcg gcggacgggt ccggaactga ttgcctgccc gacgtgtggg
     7741 cgggtcgagg tcgatttgtt ggcgctggtc agagaggttc gcgagcgact ggcgtcgatc
     7801 aaggcgccga tcagggtcgc ggtcatgggc tgcatcgtca acggcccagg cgaggccgag
     7861 ggggcggatg ttgccctgct ggccggcaag ggtaaggcgg tcatctgtgt ccgaggcgaa
     7921 ccggtgcgga cggtccccga gtccgagatg ctcgacgcac tgtatgaggc agtcatgagt
     7981 ctcgacgccc cgtaagcgcg actcaacaca gcgggcccaa ctgggagccc gtttgtcgcc
     8041 cattgtcctg ggccgccggg acgaagttcg gacgccgtgg ccatagccgt ctgtggctga
     8101 tcgcagtagc ttgcggtatg atgaccaacc atgacagaag attacgtatc agccgccgcg
     8161 cgtcacctca gggacgcaga cacactgctt gtggcaaagg cttgggacaa cgcggcctac
     8221 ttggctggtt atattgggga atgtggcgtc aaggcggtca tacaacacgc cgggttgtca
     8281 ttgcgacgga tacatcttaa tgaggttaag cccgagcatc ttctgctggc ggcagacctg
     8341 agttttgctg ccagacgcta cccgatcgat cttgaccggg aggctgccga actgtggcgg
     8401 cgatggtctg tggctctgag atacgctcga acaggtgagg tcgatgaacc agcctgtcgg
     8461 actctgctcg atcgggcgaa actcgtgttt cgaaaaacgg ttgtccaaat ggtcttggat
     8521 ggagtgttgc agacggtgcc caaatgattc atttcgagga cgttctcccg acggccttca
     8581 aaacgatcaa gaaccaccgg tcccacggct tgatcgagga ggtattggtt ctccgggatt
     8641 tgcggggtcg ggtcaggctg ttcctgaaag cccgggaaaa cagcgcggat gaacttgcca
     8701 gttctattgc cgatttggcg accagcctgg agacctccct tgctccgttc tggggcggag
     8761 tgctcgacgt cgaccggccg aagagcgagt tttacgccat tcttgagcag gtacgctccg
     8821 accgacagcc tattgagccg caatctgagt ttccctgctg gtggatcgtg gagcgttgcg
     8881 cggcgaagtc agcatggatg agccgcgagc acaagccacc ttggcccttg aacgctcaca
     8941 cccctgccat aatggcattc tactcgcaca agggtggcgt tggcaggact acttgcctgt
     9001 gcgctgccgc cgttaatctt gcgagaggcg cgaagaaggt ggcggtcgtt gatcttgatc
     9061 ttgaggcccc cgggctaggc accttgttgg cagacaatcc agttgaacat ggggtagccg
     9121 attatctctt ggaacacctc gtagctggat cgtcattcga tccagatatc aaggactatg
     9181 tcgtaaagca gtcggacaag gatctgatcg gcactgcggg agagccgatc gtctgcgttc
     9241 ctgttggacg tgtcgacgat cactacgtgg agaagctgtc tcgcatcgac tacgaattgg
     9301 ttgcgagcat gacgaatcca gggcagagcc ccttagccga cctcttgctt cagctcaagc
     9361 aggaattcga tcccgagtat gtcttcttgg actgccgtgc aggtctccat gatctcggtg
     9421 gtcttgcggt tcattgcctt tcccacgctg cggtgctcct tgggctcgat tccagtcaga
     9481 gctggcaagg cgtgcggtgc atcattcgca gcctggggct catcgatggt ccgcctcggc
     9541 cgcttctcat ggttcaagcg atggattcat atcggtcaga cgatcgggcc aaggacgcga
     9601 gggcgaggtt tctccaacag tcctacgacg ccttcacgga gcatttctat aacgaaggtg
     9661 acgtgccgga cattgcgggg gaggatgaac cgcactatcc gttccgcatt ccttacagcg
     9721 aagccctcat gggttaccag aagctcacgg aggttgtccc gctgatcacg aattcaccct
     9781 acgtggagtt tgtggcgaag atagaagaaa tggccagcaa aggcaggaat gacgatgtcc
     9841 gcgaagacag ttgacgataa tcttaagtgg cacctgttgg aggaccttag caccttcgcc
     9901 cgaagcggtg ttgatgaggt atcgggaggc gaggacttct tcaagacgca tttgccaatc
     9961 ccggagcaca ggcgggttct cgatcgtgac acgcttcttg ttctcggcgg cagaggcacc
    10021 ggtaagaccc gcttgttccg ggctctaact caagttcagg atccgagcga tctcttacca
    10081 ggacagtcgg gttcgtccca agtgcgttca gacaagtacg tgaaggcttt cactctagat
    10141 gggaccacat tcccgcgtcc cgaggtgctc cagaaggatt tcgtcgctcg cggccaggac
    10201 gatgaccaga gactccagtc tttctggctc ggtttgctcg caggcgcgtt ggctactgaa
    10261 cctttgaccc aagctgccat caaggaatca ctcccgccgg cggtgtatca ggtgctttgc
    10321 gattccttgc cagatcccaa ccgttggatg gacttggtca accaggacat tggtggaatc
    10381 agttcctcgc tggatagggc caatgatctg ctccgcgccc aatcgcgcaa tgtggtcctg
    10441 gtctacgatg atcttgacag gcttgtttcc caggtgtccg gggtctaccc tttggttcga
    10501 cagctactga ctttctggct gaacaacttc cgtcgctggt cgagcatacg atgcaaggtt
    10561 ttcttgcgga ccgacatatt cgaggcggat gaagtcgctt tcacagacag ttccaagtat
    10621 cgtcccctat ctgtgactct gcaatggacg gccgacaacc tatacagact ggtgttgaag
    10681 cggctcgtca atggagctaa gcaggaagat tggctggtgt tcgtgcgagg gaagctgagc
    10741 cgtcgcgaag actttcggaa gagtgaacgt tggggtctca ttccgatgac gtccgagtcg
    10801 cagcatcgca gcttcatgga acttctcgta ggcaagtgga tgggctcgga caaacgccgt
    10861 ggagatactt accgttggtt cttaaaccat cttcaggatt ccctcggcaa cattgccccg
    10921 cggtcatttc tcaaactgtt cgaattctcg gcgtcacgtc agcttcgcaa agatttgcct
    10981 ggcgcggggc acttgctggc tcccgaggag atcagcggtg ctctgaccga ggtatcaaaa
    11041 gatcggctgc aggaactcag agaggagtat gactggattg acgatatcgg cgccagtctg
    11101 aaagggaaga cggtacccat ggaacgtggg gagttccgcc gatgtcttgg caaggtgcca
    11161 tggcagaagt tgcccgactt cctcggcgag gacaaggatc gcttggtgca gtatctcatt
    11221 agcctcggca ttcttcgcga gacggaggac aagagaatcc atgtccccga catctacctg
    11281 tttgggcttg agttgagacg gaagggtggt attcggcgtc ctcgtgtctg acggggacgg
    11341 acagacgagg cagaaaacgc ggacttcgtc ttcctggtcg agacgttctt tggttggagc
    11401 gaggatctcg cgcacagcga cgttatcaat gctcttctgc gtggcggggc ctgcacaggg
    11461 tagctggttt ccggtttggc ggttcgtgct gggtcagatt acgcggctgg tgactcggtt
    11521 accgccggct tgttttgact cgtacatgag tttgtcggct gcgtcgatca ggctttgcaa
    11581 gggatgctgg gtatgggagg gcgtgacgta tagaaggccg gcactgaggg tggcgcgggc
    11641 ctggtcggta tgtcttccga tcgttgctgc ggcgacctgt tcgagcagcc gtttgaccgt
    11701 gtcctgagct tcggccttgc tgcaatcggg gatcagcagg atgaactcat cgccccccat
    11761 gcgggccttg atgtcctgtt ctcgggttga ggtcgtgaga cattgtgcaa tcgacctgag
    11821 cacgcggtcg ccaaagtggt gcccgtaggc gtcgttgaag gtcttgaact tgtcgacgtc
    11881 gaagaaggcg acggcgaagc tcatgccgta gcgagatgcc ttggccaggg tttgctcggc
    11941 cgcggcggcg aaaccctcgc gattgagcag gtcggtcaac tgatcgcggt tggccttgtc
    12001 catcagcgtg atattgcggt cgatgagttg gttcatttgc agcccgacgt tggctgcttc
    12061 ctgcatgagc cggtgaacgg tcttgaccag atgttcggtg ttctcggctc cttctttaga
    12121 agtctggatc agcaagtctg agagttgctt atcgagtcgt ccgccctcat cgaagaagcg
    12181 gtagtactca tcgacgccgt tttggacttc ctgcaggaac gcggcgggcc cgatctcgtt
    12241 caagccttcg gtctcgtcaa tgatgccaca caactcattt gcgtcccgac tgttccatgc
    12301 gtccaggtcg tgggggaaca gtgccgcgat gtgaatcgcc tgagcggtcg ccatgtcggc
    12361 catgaatgcc ttgaggcgtt cctggtcgtg atgaaacgcg atggcgtcca cgaagacttc
    12421 cgggagttcg agtttctgtg ccaggatgcg gccgagcccg gtgtggtcaa gatggaacag
    12481 atcatgttca cgctgcaatt gtccctgagc gtcgcaggcg gggtcggcga ggatcgccag
    12541 gtactgctgt cgggcgaccg agtacatgac cgggacggca aggtcctcga acaagccggc
    12601 cacaaaggct tcctcggcca aagccgggtt cagcttctgt gcacaacaac gggcagccac
    12661 ggccttgcag aggcatgttt cccaaaagcg ccttgactca tcgcgactca agcgtagttc
    12721 gttgtgcaac cctgccatac agtagctgat ggccatggtg cgaacggttc cgaggccgag
    12781 cagattgatg gcgtttcgga cggtggtgat tttgttgcgg atgccgaacc aaggggagtt
    12841 ggcgagggcc aggagcttgg cgctcagcga ggcgtccgcc ccgatgactt tcgcgtaatc
    12901 gctcggctcg gcgtcagggt tgtcgcggag ctcaacgagc ttcatggcaa ccgcgacggt
    12961 ggttggcagg tgggagagcg actcgacacg ggcgcggatc tgttgttcca gttcggcggg
    13021 gtcggcctcg acagcggctt cagcagttgt ctgactcatt ctgagtgcat ctcccggtct
    13081 ccagccgcct gcagcggcag gcctgtccgg cggcgtggtg ggcactcttt gacgggcgga
    13141 ggtctgttcc ccattcgacg gcaacgcccg ccgacgcttc gccagcggca gagggtcgtg
    13201 tctctcttat gatcgacata gcacgagacg tacttgacca gctacgaggc acgggtccgg
    13261 ataatcggca ggcgacctgc agagggtctg ggctgcgcaa cgggcacgtg ggggcgcttc
    13321 atggtaagcg ccttggggtg tctatggact ggtcggtcag gcgtttgcct cttggcgagg
    13381 gtcattgggt cgatggtggc ctggcgcgtc gcgagcccgg cgctcgcttg tgaagcgctc
    13441 gggtaatccc gcacagcttg ccctgtgtgg gcgaggaggg ggattggacg tgggagtcgt
    13501 tggaacgctc ccgcagatca tcggatcatc gcgagagttt ctctgtcagg tattccgtca
    13561 cgcgggactt gttgatgcgc tgctgggcac cggtgtcgcg atcgcggacg gtgacggtgc
    13621 cgtcttcctt ggtctggccg tcgatggtga agcagtaggg ggttccggcc tcgtccatgc
    13681 gggcgtagcg cttgccgatg gaggccttgg cgtcgtactg ggcgggccag cgttttcgga
    13741 cctcgcggta gattggctcg gcgatctcgg gcagcccgtc cttattgacc agggggaaga
    13801 tggcggcctt gatgggggcc agtcgcgggt ggaacttcat cagttccggg ctggggcggg
    13861 attcatcgac ggtgtatgcc tcgcagatga aggccagcgt gccgcgatcg gccccggccg
    13921 acggttcgat gacgtgcggg atgaatcgtt ccttgcgttc gtcgtcgtag tagctcaggt
    13981 ccttgccgct gcctttccac ctgggtttgc cgtccggacc gacatcgagc accagcttgc
    14041 cgtccttgtt gatcagcttg ccttccatgt ggctgcggag gtcgaagtcg ccgcggtggg
    14101 ccacgccctc cagctcgccg aaatcgccct cggccaggaa ggggaacgcg tactcgatgt
    14161 cgctggtgcc gcaactgtag tggctcagtt cctccttgcc gtgctcgcgc aggcgcagct
    14221 tgttgccggc caggccaagg ctggtgtacc aattgaatcg cacgtcgcgc cagtactcgt
    14281 accacttgtt cgactcgctg gggtggcaga agaactcgat ttccatctgc tcgaactcgc
    14341 gcgagcggaa ggtgaagttg cgcggggtga tctcgttgcg gaagctcttg ccgatctggg
    14401 cgattccgaa ggggatcttg atccgcgtgc tgtcgcagac gtttttgaag ttgacgaaga
    14461 tgccctgtgc ggtctcgggg cggaggaaag cccggttggc ctcgtccttg atcggaccgg
    14521 ggtaagtctc aagcatcagg ttgaactcgc ggggttcggt caactcgcac ttgtcgtgct
    14581 ctcccgggtg cttgcttggt ttcatcgggc actgcatctc tttgagctgg tccgcccgaa
    14641 aacgagcctt gcatgccttg cagtccacca tcatgtcgct gaacagatcg tagtggccgc
    14701 tcaccttcca cacctggggg tgcatgatga tcgagcagtc gaggccgacc atgtcctcgc
    14761 ggtcctggac catatctttc caccaggcct ccttgatgtt ccgcttgagc tccacgccca
    14821 gagggccgta gtcccagaat ccgttgatgc cgccgtagat ttcgctgctc tgaacgataa
    14881 acccgcgccg cttgcacagg gccacaattc tgtccatcac attcccggtg ggtgctgcca
    14941 tcactcattc cttccagaca atcacaacct gttcagacca agagaacagg tagtataccc
    15001 gcgcatccgt caaacccaaa gggcagcaat cgtcatgtgg aggtgcaccg atgcagggcg
    15061 gtgcggcgga tcttctttgt cgcttatgtg ccttttgagc ttcttgcggc caacaactcg
    15121 attgagccga cggaagagag ccgcgaaaag gcacaagaag ctcaaaaagc cgggggccac
    15181 gctcgacatg gagaaagcag gctcgcctcc ggagtatgcc cgctcacatg tttcgtgcgc
    15241 aggtacgttg ttcatgtgtt cggagtcggt agtttcgatc ggcatcatcg cgatacgcgt
    15301 ggctttgcag agcctcatat ccctcttccg gaccgaccac aaggttgcta gaatgaacag
    15361 gttgatatgg aggctcggcg atggtccgca tgatgatcga gttgcctgaa ggcgttctgg
    15421 cggcccttcg gaaagacccc aagagctttg ccgaggaaat gcgcctggct gccgcggtca
    15481 aatggtatga gttaggcctt gtatcccagg gccgcgcggc ggagatcgcc ggtctacctc
    15541 gccgtcagtt catcgatgcc ctgtcccggc tgagtgtctc cccgttccag tacgcgcctg
    15601 atgaggttgt gcgggaggcc gagggtgagt gagacgtggg tcgtcaacgc ctcgccggtc
    15661 atcgctttgg ccagaattgg tcaactgcga ttgcttgagg agcttgcgag cccgctcttg
    15721 ttaccggagg cggtggcagc ggaaatcctt gctgggcccg atgatgaccc tgcccgggcg
    15781 gcggtgttgt ctggctgggc ttcccgccgg tcacctgcgc aggttcccga cgcggtcaca
    15841 gaatggggtc tgggtgcggg agaatccgcc gtcttggcga tcgctctcga tctccgggcc
    15901 atcgcggtac tcgacgatgc cactgctcgc gcggctgccc gcgcattggg cgtggcgctc
    15961 atcggtactc ttggcgttgt ggtccgagcc aagcaacgcg gtctcgtgcc tttggcttct
    16021 tcggtttttg ccgacctgcg agccgccggt ctgtacctcg acgacgacgt tgttcgtgtg
    16081 actctcgaga gaatcggcga gaagtgagat ccccgaagga gcagaagccg ctctgacatg
    16141 agctgcggat tcctccgccc cgcatcggag ggcggattgc taccgaggag gactggctcc
    16201 caggggtttc gcttcgctcc acccctggct acgtcccggg gctcctccgg agcccatggc
    16261 tcaactgcct gttcgcggga tcggaatcgc cgctcgggcg atggcgatga tctcttcggg
    16321 cgtgctgacg cagtggacgt gcttgccgtg gctggggcgg gcggcggtga cgcgctcgac
    16381 cgtcggtttc cagaaatcgc cgacgatgaa gatcgggcgg gggcggatca gcttcttggc
    16441 gacgtattcc cagacaatcc cgaactcgct gagcgtcccg gtgccgcctt cgaggatcac
    16501 gtacccggcc gacatctcga tcatctgttc gatccgggcc agaaccgtct tgtgcgaaat
    16561 ctcccgatcg atatagcggt tggccttgag cggccggccg cggtagtcgc tgaagatgtc
    16621 gcaggttacc ccaatggttg agcctccagc gtccttggcc cccttggcgc tggcctccat
    16681 gatgccgtcg tagccgccgt tggcgacgac gaaacccgcc ttggccagag cgtgcccgat
    16741 cgcgtaggcc tgctcgtaca gcggctcacc gggctttggc gagtagctgc cgaaaatgca
    16801 gacaatcggc cggccgtcgg cgattgaagc ctcgtctggg gagggagttg tatcagagtg
    16861 tcgttcgggt gttttcattg ggttcgtttc gttcgcgatc ttgcaggcac aggttcagcg
    16921 gcaggtctac gccgactgcg tcggcttcga cctgccctac cttgtggcgg aatccaatcc
    16981 acattcggcg agaataaggg tgggaatcct cactgcacaa ggccccagat aggaatctgg
    17041 ggccagcgag aataagggtg ggaatcctca ctgcacaagg ccccagatag gaatctgggg
    17101 ccagcgaatc tggggccagc gaatctgggg ccagcgaatc tggggccagc aatgagcctt
    17161 gcggccatgt gctccgacct gcttgcggct cgaacgccct agtccccaag cccccggccc
    17221 ccagacccca gaccccagac cccagacccc agtctccagc ccccgctgct tcacgcatct
    17281 ggcacttcct gcacggtgat cttctggatg tacacgggtt tgacggggaa cgatttctca
    17341 ccggttccgg ggtcgcgggt cttgaccgcg ccgatcttct tgagtgtctg gatggacgat
    17401 tcatccgcga gttcaccgaa gacggtgtac tttccatcga ggaattcgca tcggtccagg
    17461 cagatgaaga actgacaact ggccgagtcg gggtcattcg ttcgcgccat ggatagtgta
    17521 ccgagctggt ggggcttgtc gttgaactcg gcccgcaaac ggcgcggccc gtttcccgtg
    17581 ccgtcgcctc gcgggcagcc gccctggatc atgaagcctt cgacaatgcg gtggaaggtt
    17641 cggttgtcat agaacccttc ctcggccagg gcgaggaaat tctccacgtg cttgggggcg
    17701 acgtcgggga agaaccgggc ggtcatcgtg ccgaactcgg tgtggaaggt ggcttgtttc
    17761 atcgtatgtc ctttcgttac gcggtctgca tgcctattcg aaccctcgag ggcggggccg
    17821 gtttcccgga gtgtacgatc ggatttcggc gagtatagtc gagctacatc ttgccggtct
    17881 catggcgctg gcaacaggca ggctcccggc gccacgcggg attgtaccgc agtcgggcct
    17941 ggtaacgcca acgaggcgga ccttccggtg tttgggattc aacggcactt ctctcgcggg
    18001 gcttggctcg ctagaatccg gttctttaac ctcgacttgt cgagagggag ctatatggag
    18061 ccttatccgt tgcttttcga gccgatctac aagcccaagt tgtggggagg ccgtcgtatt
    18121 ttcgaccatt tcaaccgcga gcccgtgggg agcgagccga tcggggagtc gtgggagctg
    18181 gtagatcttg agggggacgc gtcgcgcgcg cgtaatggtc cggccaaggg gcgaacgctg
    18241 acggagttgg tgaagcggtg ggggcgtgct ctacttggga acgcggatct gtttgaggga
    18301 cgatttccgc tgctgatcaa gtatctggac gcccgcgaga atctgagtat ccaggttcat
    18361 ccgacggagc aagtggcccg cgaacttggc gggaacgttc gagtcaagca tgaggcatgg
    18421 tatgttttgg acgccgaccc ggccggagtg atctaccatg gactcgaacc gggcgtgtcg
    18481 cgggatgttt ttgtcgaagc gatgcggacg ggccttgtgg aaggcgtcct tcgcaagatc
    18541 agcgtttcgc agggcgactg ctattatctg ccgagcggga cgccacacgc actgggggcc
    18601 ggtgtactgg tggccgaggt tcaaacgccg tcggatataa cctaccgaac ttatgattgg
    18661 ggcagagtgg acccagccag cggcaagccg cgtgaattgc atctcgagca ggcggtgcgc
    18721 tgcatcgact tcacttcacc gccggcgtcg cccatgcagc aacacctgca cctgggtacg
    18781 accatggcca cggtgacccg gctggtcgga tgtccttgtt ttgtgatgga taagctcgat
    18841 attgcccagg gggttacgca ggatgccccg atggagtcgt ttgccatctg ggtgctgtta
    18901 aagggtctgg ggcgggttga gtggggttca ggtgaggaac ggctggattg ccgtcctggg
    18961 gatgtggttc tggtaccggc agggctcggc gaggttcggg cgagggcgat tacgcctgcc
    19021 cagtggctgg aagtgaccgt cccggctcgg tgaaagcagg aggcgggcgg tccggccaca
    19081 ggcgacgcga catgttgcgt cagcccgttt ggctcgcgtg ccattctgac agacaaccgt
    19141 tcagccagtc cgattatggc aggcacttgg gcagtggggg ggctgattct catccgttcg
    19201 gttgctcgta agttgtttcc atagaatagg ttagggctga tatggcgaca actgggcctt
    19261 tggggcccga ggttggcatc gactttgctc cagggggggt gttgaaacat gcgccgaacg
    19321 cctgcgcgtg cgggttttcc ggcaatggcg cggcgagaga acaaacgatt agggaagttg
    19381 gtttgttgaa aggagagctg gcaatggcca agctgaaagt gcgtccgttg ggtgacaaag
    19441 ttttggtcaa gcgcgtggag gccgagtcga agacggcggg cggaatcgtt ctgccggata
    19501 cggccaaaga gaaacccaag cgaggcatcg tccaggcggt cggtgacggc aagcttctgg
    19561 agaacgggga gcgcagcaag atgcaggtca ataaggggga ggaggtactg ttcagcagct
    19621 atgccggaac ggaaatcaag atcgacggcg aggagtttct gatcatcgag gagagcgaga
    19681 ttctggcagt cctgtcctga gtggggccgg ccgaccggcg ataagggttc tgggtgattt
    19741 tgaggatctg ataacactct gaggagtgaa cgatacatgg cagccaagag aattgcgtat
    19801 gacatggaag cccgtgaggc gatccgccgg ggcgtgaagc aattggcgaa ggcggtgaag
    19861 gtcacgcttg gtccctgtgg tcggaacgtg gtgctggaga agtctttcgg ttctccgacg
    19921 gtcaccaagg acggtgtgac cgttgccaag gagatcgagc tggaagacgc ctatgagaac
    19981 atgggtgctc agatggtcaa ggaggttgcg agcaagacct ctcaggttgc cggcgacggc
    20041 accaccacgg cgacgatcta cgctgaggcg atttatgacg aggggctcaa gaatgtcgct
    20101 gccggcgccg acgcgatgga gctgaagcgc ggcattgagc aggcggtgga agcggtggtc
    20161 gaggaactcg gcaagatggc caccgacgtc aagaacagcg agcaggtggc ccaggtgggc
    20221 acgtgcgcgg ccaatcagaa cgcggagatc ggccagcaga tcgccaaggc gatggagaag
    20281 gtgggcaagg acggcgtgat cacggttgag gagggcaaga cccttgagac gaccgtggag
    20341 ctggtcgagg gcatgcagtt tgacaagggg tacctctcgc cgcatttcgt caacaactat
    20401 gaaacgatgg aggtggttct cgagaagccc tacatcctga ttcatgagaa gaagatctcg
    20461 gcggtgaagg atcttattcc gctgcttgag aaggttgccc gcgcaggtcg gccgctgctg
    20521 gtcatcgccg aggaggtgga gggtgaggcc ctggcgacgc tggtggtcaa caagctgcga
    20581 ggcacgctgc aatgcgccgc agtgaaggcg ccgggtttcg gcgaccgtcg caaggcgatg
    20641 ctcgaggaca ttgcgattct taccggcggc aaggcgcttt ttgaggatct gggcatcaag
    20701 ctcgaatcgg ttgatctgaa ggatcttggg caggccaaga aggtcaccat cgacaaggac
    20761 aacaccacga ttatcgaggg tgcgggttcg agcagcgcga tcagcggtcg cattcagcag
    20821 ctcaagaacg agattgaggt cacgacaagc gattacgacc gcgagaagct ggaggagcgt
    20881 cttgccaagc tggccggcgg cgtggctcag atcaacgtcg gggcggccac cgaggcggag
    20941 atgaaggaga agaaggctct ggtggaggac gcgatgcacg cctgccgggc ggcggtcgag
    21001 gaaggcattc tgccgggcgg cggcgtagcc ccgttgcgaa cgctgggagc tcttgacaag
    21061 ctggccaaga agctcaaggg ggaccagcgg acgggcgtgg atattgttcg tcgggcgctg
    21121 atggcgccga tcaggcagat tgcggagaac gcgggtctgg acggttcgat tgtctgccag
    21181 aaggtcatgg aaagcgagga caagaacttt ggctacaacg ccctgacggg cgagtacggc
    21241 gacatggtga agatgggggt gttggtcccg cgcaaggtcg agcgtgtggc gttgcagaac
    21301 gcggcatcga ttgcggcgct tctgttgacc acggacgcgg cggtggtgga gatcaaggaa
    21361 gagaagaaga agtccggcgg gccgggtggc catggtcacg gtccctattg acggaacggt
    21421 ttgttgttag agtgtgaggc gagccggctc gcaaagcggg tcggctcgct tcgtttgacg
    21481 tgtccctgag gggtgccagc gtcgttctga aagtgcagag atttcgccgg tttgaatatg
    21541 tagt
//
LOCUS       DAOTPW010000082        21544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2510_length_21544_cov_5.664759, whole genome shotgun sequence.
ACCESSION   DAOTPW010000082 DAOTPW010000000
VERSION     DAOTPW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21544
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2510_length_21544_cov_5.664759"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..1500
                     /locus_tag="P5316_09270"
     CDS             505..1500
                     /locus_tag="P5316_09270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85189.1"
                     /translation="MPPAMVEREVGASTVNIDDFIDPARPLSWSAQMIALLRSGHYRE
                     GYEILAEKMPFVENMLRPFAERLEGKENYWTGTYDWIKRFIDDSRPLRILDIGCGIGS
                     GAMEFALEGHQAWGIDVLDCMIERGREVADSLGLSQRVHLLVGDIRRLEKHFESSFFD
                     AVVACDIFEHLDDESILEVLGGLKGVMRPGGTIVIQTSPCRYYYWFDPGRKKMLAMLV
                     PLAWMPDRLFTAYVRWLDRWPLRRLRTEHTRFYRHEPGHINCMDHVHLRRLLKQAGLE
                     QVHTFAVNAHPGFKDEGCLKAGWTRRLFRGKSVACRNVFGIASVPQGAEVGAQAR"
     gene            1564..2874
                     /locus_tag="P5316_09275"
     CDS             1564..2874
                     /locus_tag="P5316_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR85190.1"
                     /translation="MSQTRNVAIEIEGLWKRFRKGGADGLLWESALRVLHRLRGRGAG
                     ARDVFWALQDVALTVHRGEAVGIIGPNGAGKSTLLKILSRVLRPDRGRVSVNGRLSGL
                     IEVGAGFHGDLSGRENIFVNGAILGMRNDEIRRKFDQIVEFSGIGEFLDMPVKRYSSG
                     MYVRLGFSIAAQMEPDVLLVDEVLSVGDVQFKAKCMDAMRGFLRRGTSVLFVSHNLAQ
                     IKRFCDLVVLLDHGRVRLEGSPDAAIAEYSRLVSDMEDANPTGIEHSHIGGRGRIDSG
                     ARITAVRILDGSGQPARTFTSGQPVRLEIDYFIDPVTGGIMEPNVVISVHMFGAGLFY
                     QFESLRDGLRLGRCVGAGTVAVRLPWLALGEGVYQIGVALADRKGLPEHDWHDRAYRI
                     NVVSDGATDGPVHQPREWQIVAGGNESGCEQPIAATSEAAGVPA"
     gene            2871..3827
                     /locus_tag="P5316_09280"
     CDS             2871..3827
                     /locus_tag="P5316_09280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR85191.1"
                     /translation="MSLSAAPVTVVIPCLNAEKTLPAAIESVLFQTVPPIEVLVVDDG
                     STDRSAAVAAAIDPRVRVLRNPSRGPGAARRLAVSEARGEYIAFVDADDTVDPTKHEK
                     QLAVLEDSDPHTLVHTGSLARWVDGSRPSYQRQGAEAAVGRCTQVIFERNPVCGASTM
                     LRRSVILELGNYQADLFGTEDFGMSLVASTCCDFVYVPEPLYIMNQHATNITRRRAHM
                     AYVHWLAQDYFRRRCPEAFAALPAESVQKYMIEPVLRTVLDSYWRRESADYQRLLRLA
                     VTIAPHDPRIRRLWKRRYVPMTVLRWWDRLTGTLRPVVGEVS"
     gene            3829..5070
                     /locus_tag="P5316_09285"
     CDS             3829..5070
                     /locus_tag="P5316_09285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR85192.1"
                     /translation="MKMESMGCGPPHLALVTANPICIGGMQTFSRFLVETACKAGWRV
                     TVALSGENVFAGVRGSLDVDRVDWVDADLCGDRRYHLRTILSRRGWFREHRPDVGLFV
                     QSSNTPFRSSVVGAVLAGTPVVMTHRTMPWIRDFIPAGRHLFRLLPGLGLHNRRQVLK
                     TRVAAALASRIVYNSEFVRREYEGIYGYPRSKGCVIVNAAPPVDGVVARRRFQDCVVI
                     GYLGRLADEKRIDVLIRAFAAMRHRQKARLLFYGDGPLRESLAKLAADLGIADRVEFR
                     RSTSDAAAAYAEMDIVSVCSRRESSSNTVLEAMAAGRAVVVSDAGGLPELIDNGQAGV
                     CVAVGDIAGMADALDRLAVDRDERIRLGRAAQALARQRHDAGRVGRQWLDLLGEVAAQ
                     RRCRRKASVDDLKAEPVASTR"
     gene            5075..6142
                     /locus_tag="P5316_09290"
     CDS             5075..6142
                     /locus_tag="P5316_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85193.1"
                     /translation="MARLMVNIDTEEEGLFRGHYPTRAWSLGHLAELPRLQGVFDRFG
                     VRPTYQVTTPVIMDRVGSGHLERFLEEGRCDVGGHLHPWVTEPLAGLPADDAHSMPCM
                     LAPALVRAKLITLTRQIRERFGVQPIAYRSGRYGSSAAHTPFLLELGYRVETSVCPFV
                     SHTPFGGPDYFDAPLAPYWLGNESMLEPEPSGRLLCVPISAGFGSPDFETARRLYRRL
                     GRTRLRHLRLVGLLHRLRLLRLIRLNPEMTGLHDMIRLCRALLRRGTELFHLTFHSSN
                     IGIDGTPYVPDREARDAFLDRLSGVLDFLVNENGVRPVTAREYYDLVCACRTSPARRE
                     PQRSADSVGAAMACAETACCT"
     gene            6155..7213
                     /locus_tag="P5316_09295"
     CDS             6155..7213
                     /locus_tag="P5316_09295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR85194.1"
                     /translation="MKIRATRPDELNAVAAFLTDGGMPHTAATLRWKFWDSPGRLNGC
                     GKSIVAEIDGRIVGHIGIMPATVHLDSPGASGVPGGWFVDWVVRRDLRSRGIGIFLLR
                     EAEKTCRALMTIQGTADTRQALPALQWVRQDGLSVFKLNVRAGGTCEEAGLGRRVATE
                     LARVVRYHPVRWPAPEGWSVTLAGESPAADPWHELDAVLARQHQSLNGAFCHFDRSAE
                     ALRWLFMAHPAGHYRLTIARDAAGPAGYIIWRTCRDSGHRIDGRIVDILAPWERPDAW
                     RWLVSVAVARLAAQGVVQISCLAGADSVLGDALRVNRFLSRQDLPLWISPLAAEFCIP
                     RRWHVTFADSDIDTASRE"
     gene            7473..9431
                     /locus_tag="P5316_09300"
     CDS             7473..9431
                     /locus_tag="P5316_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRR85195.1"
                     /translation="MSPGVVLVCDPRVGQARTLVAGVLDALGASSSENREASAVETQA
                     CAGVNWAAATVAMCSAESTSGSCAYSDGHDVLLWAGEMLLPVAWVHGGAASREAVAEV
                     VLRRLQAQGFGILEEIDGAFCGAWYEQSRRQWNVFNDKLGLIPVFYAARADRLVIGPT
                     ARVVWQATGEPLTLSEEGFCDLLRCENMTDEHTLIEGVNWLKGGHVLRWSPGHCRTQR
                     YWDFRFADSEKCDREEAIEQYTSILCGTMRRHADCRAPLLLGISGGLDSRMLLGMCAE
                     IGRLPLCFTVGWSFSDDVRYGRGLARAAGATHEWVGLEEKDLPERLTQAILATDGLHN
                     VAHMAPAATMRSYLVGHAGAVLLEGYLQGVVGGAYVPLDEDVVEDMVPHQSRWARYRL
                     HAGGDASIINGLLLPDPADRSYRRWQERIDTTWRLAPTDDPLGKAEYVVAASRSGRID
                     VLGTAMLRDSVWVRCPACDNAVLAWRERTPPRWRRGKQLFLEVIRRQFPKLARVQRTA
                     SSGLPVSGNRWLREYCWQKERLHRWWTGLGHPWTRKWGTGGNAGRAWAFATWRATGGL
                     DVLTSANARILNWVRRDRLMEVWEQAVRDPLECGPVMALATAEIMIRHLERIRPGFRG
                     VSPGQVQFVMMETPASCKPQPAWAGTAC"
     gene            9392..10975
                     /locus_tag="P5316_09305"
     CDS             9392..10975
                     /locus_tag="P5316_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR85196.1"
                     /translation="MQATAGVGGNGVLRTDDSMTVDEQRQRWTEWVLQRPEITAETRG
                     GRALLISYIFPPIGGSAAQRPAKLAKYLPRYGWDVEVLTAGHSRFPWRDDSLLADVPD
                     GCRVHRVSGREPACMARTLAKLVPHAGWSDRIEHGMHWRLSTLLQRFGLDDPQSLWVG
                     PAVRFAVRQHRRGRYDVIVSSGPPHFAHWAGLRIARRTGVPWVADVRDPLVSDFDRSL
                     TTGRKTESMRRLESQIMKHAAMVITTCPSLADEFRRRFADRRPDSIRCITNGFDRDDL
                     LVALDVQPQGRDLFVFVAAGAFYGRREIGRIVTPLQAVLAGHPEWTDRVRLVIAGTLD
                     AQQQREWQQHSPPWLTLAGYLDHPAVVRLAAGAACTIVVVPQCKHGETSIPGKTFELL
                     GLPTHLLALVPTNGDTARIVREAGASTVVAFEDGPEVAAAMERIIADHFAGRLECGRS
                     WAAVDRYDRACAARGFAECLNVVIDGRASLPACAVRTSDIESGATGTADNTMVYAGLG
                     EVSASPTDLLNVSISPQLR"
     gene            11101..12372
                     /locus_tag="P5316_09310"
     CDS             11101..12372
                     /locus_tag="P5316_09310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR85197.1"
                     /translation="MGRMVRQLMFVSDAPSFGGAERYIIAMALAARRRGLDASICWLP
                     RPGCGQAVFDIARENGIRLDVVDPRRTASLGGLAREFTAVLRRENPDGVIINACGRPR
                     FWILPWLARRVSVPAAWVHQMVDACDHRRLPARWLGGRLEGLHCWRLPQMLRHRLAGT
                     GATAIVTLNADDRERIVRWQGVCRDKVFAIPHGVDCVRFEFDATGRQRWHHEWGIDAV
                     SPRPFVVGAAGRLSREKGVDLLIEAASIARREGLSLLLVIAGRGSEQDPLVRLAADRG
                     LAGAIRFVSFVEDMPSFYSALDAFVLSSRTESFGLALAEAMACRRPVIATPTSGATRQ
                     IRHLHNGWLLRGFEPSELARAITALAADVEGRRRMGGNGRESVMRCFSIELTLERTLR
                     ALRGSARERSHLRWPGMNESPFVSMTAEECA"
     gene            12369..13676
                     /locus_tag="P5316_09315"
     CDS             12369..13676
                     /locus_tag="P5316_09315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR85198.1"
                     /translation="MRLLVPLPFDVSNLAHGRNLRIAHVLPALQSNCEVLCVASNEHI
                     AAAARRVMPGVEVQVAGRVKGGCPENHAFHCEPFLVRRSLSFLGYDEGFAEVIARLGV
                     SADVVAGFDMPSVAPLLAAAETRGENRRPRIVCDLIDDPWLLHRSATRAYRRSLTGMK
                     AAAAMQVVRCRVLSRFDALTAVAPQDASRLARVSGRPAVVVPNGVEASTSAAERRPRE
                     PLVVFTGNMSFPANETAACFVARKVWPLVLASYRCGPAGSSHCRVPTLAIVGANPTPD
                     VCKLANLSNVTVTGWVPSVRDWLERAQTAVVPMLGGTGIKNKILEACAAGCPVVATSI
                     ATGGLPVGEDFGIIIADGPERIAAELLSLLVDPTRARRIGSAGLDMVRARFSWSRVAV
                     EFLAVLRGETAAPADSDGGSDKQDETYRRCEVEEMEALTHAPS"
     gene            13663..15078
                     /locus_tag="P5316_09320"
     CDS             13663..15078
                     /locus_tag="P5316_09320"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRR85199.1"
                     /translation="MPLRSIAFLLYFFGSAGASLLVPIAGVLCYIVLYNVYPQSTWWG
                     KYLEPLGIRYAYVIAACLTVGVIVNLNRLRYGRQVAHPVEWGMLLVFLSMLMSGVTGV
                     AWNERTDFVIDKMWKVFLFAFMMSHVVVTRRHVWLLLIVFTVMSLYLGYEAKNAPPGA
                     FLQNRLDGIGGPDFRESTGLAIHLLALLPFTAVLLWQKYWRFRVLAFFAACYGVNAIL
                     LCRARSAFIAGLVAGLTAVWYVPRRYRSWAACVLVLGLLGSVKLSDQWFWERMKTIVS
                     SREEREVSSAARLLIWASAWEMIKDRPLGVGIGQFQRMTNRYTTPELRRIYGNPATES
                     VNMIARDAHNSFILCAAETGIPGLACFLLTLIMAWGTLSRLRRRARRYLSDPAMFEML
                     VFANRMALLVYLVGGMFTSRFYTEGFWWMVILPVCLKRAVENEIRDEVCEKATVRALL
                     DDWIPRGDRLQPELVMGGQPA"
     gene            15075..16331
                     /locus_tag="P5316_09325"
     CDS             15075..16331
                     /locus_tag="P5316_09325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR85200.1"
                     /translation="MTTGTSAGPGRPTDARSPSSLRRIRVLHVIPSLFSGGMERFLIE
                     LMRASTPVGGAGEGCRVTHGVCILRAADDRLFSQCKSLATTWVLGRHKARDWSCWKQI
                     RQVIRDFEPDVVEALSTGAWVDAARAVGRSRSTKLSLVFHGQMDTVPAGRIRRWLNRW
                     AVGRAATVISVSREAADRLIREWHIPAAKMVAVPNGVDVNRFHPAASADERLRVRRRL
                     EIPDGSKVAICVANLVPIKAIDVLLDAWRQLSADQRDARLLLVGDGPLRREWHNLAET
                     TECHKSVSFLGNRDDVPALLRASDLFVSSSRYEACSMAILEAMASGLAVVATDVGGNG
                     ELVEPGRTGWLVPPDRADLLAQALAAAFDDDQARIRFGQTARGVVMERHDLEACARRY
                     STIYHSLALPHTKRPATAPEEPECAA"
     gene            16319..18313
                     /gene="asnB"
                     /locus_tag="P5316_09330"
     CDS             16319..18313
                     /gene="asnB"
                     /locus_tag="P5316_09330"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRR85201.1"
                     /translation="MCGIAGLVRFAGLRPHETSAGLDMAAFLRHRGPDEMGTYVDGHA
                     SLGHARLSIIDLADGRQPMSNEDGTIQVVFNGEIYNHLELAEQLRARGHVLRTRCDTE
                     VLAHLYEDHGDSFPEYLNGMFAIAIWDSRSRRLVLVRDRLGVKPLFWTDDGRRVAFGS
                     ELKAVLACGDLERRINPLALADYLTFGHVPAPKTIFSGVRKLEPGCMAVCTGSGTSVH
                     RWWDIPFGDADSAKPDERAGRRWTEDFASLLENAVDMRLMSDVPLGAFLSGGVDSSAV
                     VAAMCRCSQDPVLTHTVGFDEPCCDEREAARVVAHRLDTDHREVLVRSDAAAAIERLT
                     RHFDEPFADSCAVPLLRLSEITRQRVTVALSGDGGDEMLAGYRRYRFDLAEATIREMW
                     PGWLRRSTAGAMGRIYPKADWLPRSLRAKATLCNLACDDITAHLRSTSLAAGMLPDLL
                     LRPEMRCRVVGYDPFARGRDLFARCPSSLLNRLLYVDMKTLMVDDILTKVDRASMAVG
                     LEVRTPLLDYRIVELSARMPAWLKLDGRRDKVVLRDTVRRWLGPDVAGRRKKGFDVPV
                     NEWFRGPLRPMASDLLMSPEAVCSQWIDQVAVRALFARHQSGISANGHILWALLSLEL
                     WARQYARGPGPVREGQDGRILRRGTGVRSPEAAVCGGMPA"
     gene            18310..19563
                     /locus_tag="P5316_09335"
     CDS             18310..19563
                     /locus_tag="P5316_09335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR85202.1"
                     /translation="MNILTTTLCYPTLARPDQGIFIQRRAEALAQHPDVSLSVVSPQP
                     WCPLLRCGEQTIARNEPLPVTYPRLVSIPMIGWATDGVAYAGTLLRVLARQRQAGLPP
                     VDLIDAHFVYPDGVGAWLAGRRLGIPVVVTVRGKIVRLSRRAFRRMQIAAMLRGVDAR
                     IAVSRSLAGWVHKVAGSDLNVDVVPNGIRPDVYRLVDRQWARIALGWHQTARYVLAVG
                     HLQRLKGFDRLLVVLPALREALGDVRLVLVGSRRGEWWFARKVLRMIERCNHEAGCRC
                     VEFLGFVDSERLNLLYNAADVMVNSSRIEGWNNSISEALAAGTPVVATDVGGNPEQIS
                     SDELGMIVRDGDLDALAGALRSALVRPWNRALIASSGGARTWAQVAAEVHAVFERVLA
                     ARAAPAGRQSPVGIDASAAMAEATT"
     gene            19560..20963
                     /locus_tag="P5316_09340"
     CDS             19560..20963
                     /locus_tag="P5316_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HRR85203.1"
                     /translation="MNRWFAGQVFWPLTERLCRRDTMRRLAELNRTQHLPPEVICEIQ
                     ERKLRRLLSTASEHCPFHSRRIRQAGIDANDPRLGLSALGLLPLLTREDIREHRDEMT
                     WFGCPRGGPRLYNTGGSSGEPLKFYFDRFRQAADGGARWRARQWWGVQPGDPEILLWG
                     APIELAANDRLRRWRDALLNQYVLNAFDMTDTTMDAYIDRIRAVRPVCLYGYAGSLAL
                     LSRHALTKGLAPGSLGSPRLRAVFVTGEVLVEPDREVIASAFGAPVVIEYGCRDGGLL
                     ALGCPAGRLHIPQENVVIELLDEQGEPIAAGDVGEVVVTHLETRAMPLIRYRTGDLAR
                     AWPVSGGQCECGVSLAALAEVRGRLTDQIVCRAGGQIRRMHALALIYVLREVDGIRQF
                     RITQRSLDELDVEVVPTARFSVDSERAILLGLHRRLGSDVRIRLNRRDHIPATASGKH
                     ACVISQVGQELGPGLPG"
BASE COUNT         3448 a         6227 c         7500 g         4369 t
ORIGIN      
        1 tcggtggagg attgttatcg tcatggatgc cgaatgcgga aaggattccg cggggaatgc
       61 ggcccggaaa ggattccggg ccgagcggaa aggattccgc agcgtgtacg gcccggaaag
      121 gattccgggc cgagcggaaa ggattccgcg ggggatgcgg cccggaaagg attccgggcc
      181 gagcggattc atttggaggc gcatgcccac gcagagccgt gggcatggca cccggcggac
      241 ttgccctgcc tgcttcaaag gcgcatgccc gcgcaaagtc gtgggaatgg cactcggtgc
      301 tttttgcggc ggcacggagg accgcgatca ctacgcccga taatgacttc gttggcagcc
      361 tccaggcgaa gagcacttat agggccggct tatctcggac cttggaacat ccgacaaaga
      421 caggatcagc agtgtagtgg gcgtgtgctg ccgggaaggc aggccgcgcc gagcctgcgc
      481 cgaacgcaaa cggagctgga agacatgcca ccagcgatgg tagaacgcga ggttggggcc
      541 tcgaccgtta atatcgacga tttcatcgat ccggcgcggc cgctgagctg gtcggcgcag
      601 atgattgcgc tgctgcggtc gggccactat cgggaggggt atgagatcct ggccgagaag
      661 atgccttttg tcgagaacat gctgcggccg ttcgccgagc ggctcgaggg caaggagaac
      721 tactggaccg gcacctacga ttggatcaag cgtttcattg atgacagccg gccgctgagg
      781 attctggata tcggatgcgg catcgggtcg ggagcgatgg agtttgctct cgaaggccac
      841 caggcgtggg ggatcgacgt gttggactgc atgatcgagc ggggccgcga ggtggccgat
      901 tcgcttgggc tctcgcagcg agttcacctg ctggtcggag acatccggcg gcttgagaag
      961 cactttgagt cgtctttttt tgatgcggtg gtggcgtgcg atatctttga gcacttggac
     1021 gacgagtcca ttctggaggt acttggcgga ctgaaggggg tgatgcggcc gggcggaacc
     1081 atcgtcattc agacctcgcc gtgccgatac tactactggt tcgacccggg tcgcaagaag
     1141 atgctcgcca tgctggtacc gctggcctgg atgccggacc ggttgttcac cgcgtacgtg
     1201 cgatggctgg atcgttggcc gcttcgtcgc ttgcgcacgg agcacacgcg tttctatcgg
     1261 cacgagcctg gacacattaa ttgcatggac cacgttcatc tgcgcaggct gctcaagcaa
     1321 gccgggctgg agcaggttca cacttttgcg gtcaacgccc accccggctt caaggatgag
     1381 ggctgtctca aggccggttg gacgagacgt ctgttcaggg ggaagtcggt tgcctgtcga
     1441 aatgtgttcg ggatcgcgag cgtgccgcag ggagcggagg tgggagcgca ggcccgctga
     1501 cccgaaggtt cgaccgtcgg caagacacgt gaatcgccgc ggggaggcag gtgcgaagca
     1561 ggtatgagtc aaacacggaa cgttgcgatc gagatcgaag gcttgtggaa acggtttcgc
     1621 aagggcgggg ccgacgggtt gttgtgggaa tccgcgcttc gcgttttgca ccggttgcgt
     1681 ggtcgcggtg ccggggcgcg ggacgtcttc tgggctttgc aggacgttgc tctgacggtg
     1741 caccgcggtg aagccgtcgg catcatcggt ccgaacggtg cgggcaagag cacgttgctg
     1801 aagattctgt cacgggttct aaggccggac cgagggcggg tcagcgtcaa cggtcggctc
     1861 agcggtttga tcgaggtcgg tgccggtttt cacggcgatt tgagcgggcg cgagaacatc
     1921 ttcgtcaacg gcgcgatcct cggcatgcgg aacgacgaaa tccgtcgcaa gttcgatcag
     1981 attgttgaat tctcggggat cggcgagttc ctcgacatgc cggtcaagcg ttactcctcg
     2041 ggcatgtacg tccgcctggg tttctcgatc gccgcgcaga tggaacccga cgtgctgctg
     2101 gtggacgagg ttctttcggt gggggacgtg cagttcaagg ccaagtgcat ggacgcgatg
     2161 cgtggttttc ttcgacgggg aacgtcggtg ctgttcgtgt cgcacaacct cgcccagatc
     2221 aagcggtttt gcgacctggt ggtgttgctg gaccacggcc gggtgcggct tgaaggatcg
     2281 cccgacgcgg ccatcgccga atacagccgc ctggtgagcg acatggagga cgccaatcct
     2341 accggcatcg agcattctca tatcggcggt cgcggtcgaa tcgacagcgg ggcccggatt
     2401 acggccgttc gcatccttga cggaagcggt caaccggccc ggacgttcac gtccggtcag
     2461 cctgtgcgcc tggaaattga ctatttcatc gatcctgtca ccggcgggat tatggagcct
     2521 aacgtcgtga tttcggttca tatgttcggt gcggggcttt tctatcaatt cgaatcgctg
     2581 cgtgacggcc ttcgcctggg gcgatgtgtg ggagccggca cggtagcggt tcgcctgccg
     2641 tggttggcgc tcggggaggg cgtgtatcag atcggagtgg cactggccga ccggaaaggg
     2701 ctgcccgagc acgactggca cgatcgcgcg taccggatca acgtcgtgtc cgacggcgcg
     2761 accgacggac cggtgcatca gccgcgcgag tggcagattg ttgccggggg caatgaatcg
     2821 ggatgcgagc agccgatcgc ggcaaccagc gaggccgcgg gggtgccggc gtgagcctgt
     2881 ccgctgcacc ggttaccgtc gttattccgt gcctgaacgc tgagaagacg cttcccgccg
     2941 ccatcgagag tgtcttgttt cagacggtgc cgccgatcga agtgctggtt gttgatgacg
     3001 gcagcactga ccgaagcgcg gcggttgcag cggcgatcga tcctcgggtg cgcgtgctca
     3061 gaaacccgtc gcgagggccg ggtgcggcgc ggcggctggc ggtcagcgag gctcgcggcg
     3121 agtacatcgc ctttgtcgac gccgatgata cggttgatcc gacgaagcat gagaagcagc
     3181 ttgcggtgct ggaagacagc gaccctcaca cgctggttca tacgggctcg ctcgcccgct
     3241 gggtggatgg cagccgccca tcgtaccagc ggcaaggtgc ggaggcggcc gtcgggcgat
     3301 gcacgcaggt cattttcgag cgtaatccgg tctgcggggc gtcgacgatg ctacggcgct
     3361 ccgtgattct cgaactgggc aactatcagg cggatctgtt cggcacggag gatttcggca
     3421 tgtcgctcgt agcctccact tgctgcgact tcgtctatgt ccccgagccg ttgtatatca
     3481 tgaaccagca tgcgacgaac atcacccggc gccgggcgca tatggcctac gttcactggc
     3541 tggcgcaaga ttacttccgg cggcgctgcc cggaggcgtt tgcggcgctt ccggccgagt
     3601 cggttcagaa gtacatgatc gaaccggtct tgcggacggt gctggattca tattggcggc
     3661 gtgagtcggc cgactatcag cgtcttcttc ggcttgcggt gacgattgcg ccgcacgatc
     3721 cgcggattcg gcgactctgg aagcgacgat acgttccgat gacagtgttg cgatggtggg
     3781 atcggttgac aggcacgctg cggccggtgg tcggggaggt ctcctgagat gaagatggag
     3841 tccatgggat gcggaccgcc gcacctggcg ctggtcacgg cgaacccaat ttgcatcggg
     3901 ggcatgcaga cgttctcgcg ttttcttgtc gaaaccgcat gtaaggcggg ctggcgagtc
     3961 accgttgcat tgtcgggcga gaatgttttc gccggcgttc gtggctcgct tgacgtagac
     4021 cgcgtcgact gggtcgacgc ggacctttgc ggcgaccggc ggtatcattt gcggacgatt
     4081 ctttcccggc gtgggtggtt tcgcgagcat cggcccgacg tcgggttgtt tgtccaatcg
     4141 tccaatacgc cgttccgctc gtcggtcgtc ggggccgtgc tggccggcac gccggtggtg
     4201 atgacgcatc ggacgatgcc ctggatccgg gacttcattc ctgcggggcg tcatcttttt
     4261 cgattgttgc ccgggcttgg cctgcacaac cgtcgccagg ttctcaagac ccgagtcgcc
     4321 gcggccctgg cgagccggat cgtctacaac agtgagttcg tccggcggga atacgagggc
     4381 atctacggat atccgcgaag taagggctgc gtcatcgtga acgccgcgcc gccggtcgac
     4441 ggcgtcgttg cccggcgtcg gtttcaggac tgtgtggtca tcggctattt gggtcgcctg
     4501 gcggacgaga agcggattga cgtgttgatc cgcgcgtttg cggcgatgcg acatcggcag
     4561 aaggctcgac tgctgttcta cggcgacggg ccgctgcgtg aatcgctggc gaagcttgcc
     4621 gccgacctgg gtatcgccga tcgcgtggaa ttccgacgga gtacgtcgga tgcggcggcg
     4681 gcgtatgctg agatggatat tgtgtcggtg tgctcgcggc gggagtcgtc gagcaacacg
     4741 gtgctggagg cgatggcagc cggcagggcg gtagtcgtgt cggacgccgg gggcttgccc
     4801 gaactgatcg acaatggtca agccggcgtc tgtgtggcag tgggcgacat tgccgggatg
     4861 gctgacgcgc tggatcgcct ggcggtggac cgggacgaac gcattcgttt gggacgggca
     4921 gctcaagctc ttgcgcggca gcgtcacgac gcgggccgcg tgggcagaca atggctcgat
     4981 ttgcttgggg aggttgcggc acaacgacgt tgccggcgca aagcctcggt tgacgacttg
     5041 aaggccgagc cggtcgcgtc cacgagatag gaccatggcc aggttgatgg tcaacattga
     5101 tacggaggag gaaggccttt tcagggggca ctatccaacg cgcgcgtggt cccttggaca
     5161 cctggccgag ttgcctcggt tacagggagt tttcgaccga tttggtgtgc ggccgacgta
     5221 tcaggtgacc acaccggtga tcatggatcg cgtcggcagc gggcacctgg agcgtttcct
     5281 ggaagaaggc cgttgcgacg tcggcggcca cctgcacccg tgggtgaccg agcccctcgc
     5341 gggattgcct gccgacgatg ctcactcgat gccgtgcatg ctggctccgg ctctggtgcg
     5401 agcgaagctg atcaccttga cgcggcagat ccgcgagcgt ttcggcgttc aaccgatcgc
     5461 ttaccgatcg gggcgctacg gcagcagtgc cgcgcacaca ccttttcttc ttgagctggg
     5521 ctatcgcgtc gagaccagcg tgtgcccgtt cgtatcgcac actccgtttg gcggtcccga
     5581 ctactttgat gctccgctgg cgccgtattg gctgggcaat gagagcatgc tcgaaccgga
     5641 acccagcggc cgactgctct gcgtgccgat cagcgccggg ttcggcagtc cagatttcga
     5701 gaccgcacgt cggttgtatc gccgtctcgg gagaacgcga ttgcggcatt tgcgtctcgt
     5761 cggtcttctg caccggctgc gattgctccg gttgatccgg ctcaaccccg aaatgacggg
     5821 cctgcacgat atgatccgtc tttgccgggc gttgttacgg cggggcaccg aactgtttca
     5881 tctcacgttt cacagctcga acatcggcat cgacggcacg ccctatgtgc ccgatcgcga
     5941 ggcgagggac gcgtttctgg atcgactgtc aggggtgctc gatttcctgg tgaacgagaa
     6001 cggcgttcgg ccggtgacgg cgcgcgagta ctacgatctt gtttgcgcgt gtcggacgag
     6061 cccggctcga cgtgaaccgc aacgcagcgc tgattcggtg ggtgcggcca tggcctgcgc
     6121 cgagaccgcg tgttgcacat gagcacgtga acacatgaag attcgagcga ccagacccga
     6181 tgagctgaac gcggtggcgg cttttttgac cgacggcggc atgcctcaca ccgccgccac
     6241 gctgcgatgg aagttctggg attcgcccgg gcgcctgaac ggctgcggca agtccatcgt
     6301 cgccgagatt gacggcagga ttgtcggcca catcggaatc atgcccgcga ccgtccacct
     6361 tgactcgccg ggggcgtcag gagtgcccgg aggatggttt gtggattggg tggttcgcag
     6421 ggatcttcgc agtcgtggca ttggcatttt tctgcttcgc gaggcggaga agacgtgccg
     6481 agccctgatg accattcagg gtacggcgga tacccgacag gcgttgcctg ctctgcagtg
     6541 ggttcggcag gatggcttgt cggtattcaa gctcaatgtc cgggcaggcg gcacctgcga
     6601 ggaggccggg ctgggtcggc gagtcgccac ggagttggct cgtgtcgttc gctaccaccc
     6661 ggtgcgatgg cccgctccgg agggctggtc tgtgacgctg gcgggagaat cgccggctgc
     6721 tgatccttgg cacgagcttg atgccgtttt ggcgcggcag catcagagtc tcaacggggc
     6781 attctgccat ttcgatcgct cggccgaggc tctgcgctgg ttattcatgg cccacccggc
     6841 cgggcattac cgtttgacga ttgcacgcga cgcggccggg cctgccggct acatcatctg
     6901 gcggacttgc cgagactccg gccaccgaat cgacggtcgc attgtggata ttctggcgcc
     6961 gtgggagcgc ccggatgcct ggcgatggtt ggtgagcgtt gcggttgcac ggttggcagc
     7021 acagggtgtt gtgcaaatca gttgcctggc cggtgctgat tcggtgctgg gcgacgcgct
     7081 gcgggtcaac cgcttcctgt cgcgacagga tctgccgctg tggatcagcc cgctggccgc
     7141 cgagttctgt attccccgac gctggcacgt caccttcgcc gatagtgaca tcgacacggc
     7201 gagcagggaa tgacccatga agtggggccg acgcccccgt cggtcctccc gatcaacgcg
     7261 ggtcgttcgg ttctgcatta tcgatccgat agcctcagat ggtctttcca acgaccgata
     7321 tagtgatgac gcactggtgc aggggtcggt gtgcgcgcgg cccatgcact ggcggacagg
     7381 cagtcagcgg cacccgtgca cagccggccg acggctgatg cggaggttgg tcgctacgag
     7441 ggcacgcgga gtatcgcgga ggagactctg tattgagtcc gggagttgtt ctggtttgtg
     7501 atccgcgagt cgggcaggcc cgcacgctgg tggccggagt gctcgatgcg ctcggcgctt
     7561 cgtcgtcgga gaaccgcgaa gccagtgcgg ttgaaaccca ggcttgcgcc ggggtcaact
     7621 gggcggcagc gacggtggcg atgtgctcgg cagagtcgac atcagggtcc tgtgcttaca
     7681 gcgacggtca tgacgtgctg ctctgggccg gtgagatgct ccttcccgtg gcttgggtcc
     7741 acgggggggc agcctcgcgt gaggcggtgg ccgaagtcgt tctgcgtcgc ctgcaagccc
     7801 aagggttcgg cattctggaa gagatcgacg gcgcattctg cggtgcatgg tacgagcagt
     7861 cgcgccgtca gtggaacgtg ttcaatgaca agctcgggct gattcccgtg ttctatgccg
     7921 cccgagccga tcgcctggtg atcggtccga cggcacgtgt ggtgtggcaa gcgacaggtg
     7981 agccgctgac tctgagcgag gagggctttt gcgacctgct ccgctgcgag aacatgacgg
     8041 acgaacacac gctgattgaa ggcgtcaatt ggctcaaggg cggccacgtg ctgcgctggt
     8101 cgccggggca ttgccgaacc cagcgatact gggattttcg atttgcggac tcagagaagt
     8161 gtgatcggga agaagccatc gagcagtaca cgtcgattct ctgtggcacg atgcggcgtc
     8221 atgcggattg cagggcaccc ttgctgttgg ggatcagcgg agggctcgat tcgaggatgt
     8281 tgctgggcat gtgcgcggag atcggccggc tgccgttgtg tttcaccgtt ggctggtcgt
     8341 tttccgacga cgtccgctat ggccgagggc tggcacgggc ggcgggtgcg acgcacgaat
     8401 gggttgggct tgaggagaag gatctgccgg agcggctcac ccaggcgatc cttgccacgg
     8461 acgggctgca caacgttgcc cacatggctc ctgcggccac aatgagaagc tacctcgtcg
     8521 gacacgccgg agccgtgctg ttggagggct accttcaggg cgtggtcggc ggagcgtacg
     8581 tgcctctgga cgaagacgtc gtcgaggata tggtgccgca tcaaagccgc tgggctcgct
     8641 acaggctgca tgccggcggc gatgcgtcga tcatcaacgg tctgttgctg cccgatccgg
     8701 cggaccggag ctaccgccgc tggcaagaac gaatcgacac gacctggcgt ctggctccga
     8761 cggacgatcc gctgggcaag gcagaatacg tcgttgccgc ctctcgcagc ggacggattg
     8821 acgttctcgg aacggccatg ctccgggaca gcgtctgggt gcgctgtccg gcgtgcgaca
     8881 atgccgtgct tgcgtggcgc gagcggacgc cgccgcgatg gcgccggggc aagcagcttt
     8941 tcctggaggt gatacggcgg cagtttccca agctggctcg cgtacagcgg acggcttcga
     9001 gtggtttgcc cgtcagcggg aatcgttggc ttcgcgagta ctgctggcag aaggagagat
     9061 tgcaccgttg gtggaccggg ctggggcatc cgtggacgcg caagtggggc accggcggta
     9121 acgcggggcg agcctgggcg ttcgcgacat ggcgggcgac gggcggtcta gatgtgctga
     9181 cgtccgcaaa cgcccggatc ctgaactggg ttcgtcgaga tcggttgatg gaagtgtggg
     9241 agcaggcggt gcgtgatccg ctggagtgcg ggccggtgat ggccctggca acggcggaga
     9301 tcatgatccg gcacctcgag cggattcgcc caggtttccg gggggtatca ccgggccagg
     9361 ttcagttcgt gatgatggag acgccggctt cgtgcaagcc acagccggcg tgggcgggaa
     9421 cggcgtgcta agaacggacg attcgatgac ggttgatgag cagcgacaac ggtggaccga
     9481 atgggttctg caacggccgg agatcacggc cgaaacgcgc ggcggtcgtg cgctgttgat
     9541 cagctacatc ttcccgccca tcggcgggag cgccgcacag cgaccggcca agctggccaa
     9601 gtacctgcct cgttacgggt gggatgttga ggtgctcacg gccggccaca gtcgctttcc
     9661 gtggcgagat gattcgcttc ttgccgatgt tcctgacggg tgccgtgtgc atcgggtctc
     9721 cggccgtgag ccggcttgca tggcgagaac gctggcgaag ctcgttcccc atgccggatg
     9781 gtctgatcga atcgaacacg gcatgcactg gcgactatcc actctcttgc agcggttcgg
     9841 gcttgacgac ccgcaatcgc tgtgggttgg accggccgtg cgctttgccg tccggcaaca
     9901 tcgcaggggc cgttacgacg tgatcgtctc gagcggtcct ccgcatttcg cgcattgggc
     9961 ggggctccga atcgcccggc ggacgggcgt accctgggtg gccgacgttc gcgaccccct
    10021 ggtcagcgat ttcgaccggt cgctcacgac agggagaaag acggagtcca tgcggcgtct
    10081 ggaatcgcag atcatgaagc atgctgcgat ggtgatcacc acgtgtccct cgctggcgga
    10141 cgagttccgg cggcgtttcg ccgatcgtcg gcctgattcg atccgctgca tcaccaacgg
    10201 atttgaccgc gacgacctgc tggtcgctct cgacgtgcag ccccagggtc gggatctgtt
    10261 tgtgttcgtt gccgcgggtg cgttctatgg tcgccgggaa atcggccgca tcgtgacgcc
    10321 tttgcaggcc gttctggccg gccacccgga atggacggat cgtgtgcgtc tggtgattgc
    10381 cggaacgctc gacgcccaac agcagcggga atggcaacag cacagccccc cctggttgac
    10441 gctggccgga tacctggacc acccggccgt cgtgcgcctg gccgccgggg ccgcatgcac
    10501 catcgtggtc gtgccacagt gcaaacacgg cgaaacgagc attccgggaa agaccttcga
    10561 actgctgggg ttgccgaccc acttgctggc tctcgtaccg acaaacggag acaccgcccg
    10621 catcgttcgc gaggccgggg cgtcgacggt agtcgctttc gaggatggtc cggaggtagc
    10681 ggcggccatg gaacggatta ttgccgacca ttttgccgga cggctcgaat gcggccggag
    10741 ttgggctgcg gttgaccgtt atgaccgggc ttgtgcggcg aggggttttg cggagtgctt
    10801 gaacgtcgtg atcgacggaa gagcttcgct tccagcttgt gccgtccgaa cgtccgatat
    10861 cgagtcgggc gccacgggaa ccgccgataa taccatggtg tatgcgggtt tgggtgaggt
    10921 ttccgcttcg ccgaccgact tgctcaacgt ctcaatatca cctcaactgc gatagccaaa
    10981 gaccgatatt tatcgcatgg tgttccaacg cgccgggtgg tctctgcgcg ctggtcccag
    11041 tgttgacacg tggtctgcga accggtccgg cgagatgccg gccgcgaaga gtgagactgc
    11101 atggggcgga tggttcgtca actcatgttc gtcagcgacg ctccgagctt cggcggcgcg
    11161 gagcgataca tcatcgcgat ggctttggcg gcgagacggc gaggtcttga tgcctcgata
    11221 tgctggctgc cccggccggg ttgcgggcag gcggtgttcg atatcgctcg ggagaacggc
    11281 attcgactcg atgtcgtgga tccgcggcgt accgccagtc ttggcggtct ggcgcgagag
    11341 ttcaccgccg tgcttcggcg agagaaccct gacggcgtga tcatcaacgc gtgcggccgg
    11401 ccgcgattct ggatcctgcc gtggttggcg aggcgggtgt ccgttccggc ggcatgggtt
    11461 caccagatgg tcgatgcttg tgaccatcgt cgcctgccgg ccaggtggtt gggcgggcgg
    11521 cttgaggggc tgcactgctg gcggctgccg caaatgctca ggcacagact ggcgggcacg
    11581 ggggcaacgg cgatcgtcac gctcaacgca gacgatcgcg agcgaatcgt ccgttggcaa
    11641 ggcgtttgtc gcgacaaggt attcgcgatt ccgcacggcg tggattgcgt gcgtttcgag
    11701 ttcgatgcga cgggccgtca gagatggcat catgagtggg gcatcgatgc ggtttcgcct
    11761 cgcccgtttg tcgtcggcgc ggccggaaga ctctcgcgtg agaagggcgt tgatttatta
    11821 atcgaggcgg cgtcgattgc ccggcgggag gggttgtccc ttctcctggt cattgccggc
    11881 cggggcagcg agcaagatcc gctggtccgg ctggccgccg atcggggcct ggccggcgcg
    11941 attcgattcg tgtcgttcgt cgaggacatg ccgtcttttt acagcgcgct ggatgcgttc
    12001 gtactgagca gccgcaccga gtcgttcggc ctggctctgg ccgaggcgat ggcgtgtcgg
    12061 cggccggtga tcgcaacgcc gacttccggc gcgacccggc agatcaggca cttgcacaac
    12121 gggtggctgc tccgcggctt cgagccgtcg gaactggccc gggcgatcac cgcactcgcc
    12181 gccgatgttg aaggtcgtcg gcggatggga ggcaacggac gcgagtcggt catgcggtgc
    12241 ttcagcatcg agctgactct ggagcgtacg ctgcgggcgc ttcgcggcag cgctcgcgaa
    12301 cggtcgcact tgcgttggcc gggcatgaat gaatcacctt tcgtgagcat gactgccgag
    12361 gagtgcgcat gaggctgctg gttccgctgc cttttgacgt ttcgaacctg gcccacggcc
    12421 ggaacctgcg tattgcgcac gttctgcccg cgctgcaatc gaattgcgaa gtcctttgcg
    12481 tggcgtccaa cgagcacatt gcggccgccg ctcgtcgagt catgcctggt gtggaggtgc
    12541 aggttgccgg cagggtcaag gggggttgtc ctgagaacca tgcttttcac tgtgagccgt
    12601 ttctggttcg tcgatcgctg tcgttcctgg gttatgacga gggttttgcc gaggtaattg
    12661 cccgcctggg cgtttcggca gacgtggttg cgggttttga catgccctcg gttgctcctt
    12721 tgctcgcagc ggcagagacg cggggtgaaa accggcggcc tcgaatcgtt tgcgatctga
    12781 tcgacgaccc gtggttactg catcgcagcg cgacgcgggc ttatcgccga tctttgaccg
    12841 gcatgaaggc ggccgctgcc atgcaagtcg tgcgctgccg tgtcctgagc cgctttgatg
    12901 cgctgacggc cgtggctccg caggatgctt cgcgccttgc gcgagtttcg ggacggccgg
    12961 ccgtggtagt ccccaacggc gtggaggcca gtacgagcgc agccgagcgg cggcctcgcg
    13021 aaccgctggt ggtcttcacc ggcaacatga gttttcccgc caatgagacc gcggcgtgct
    13081 tcgtggcccg caaggtctgg ccccttgtgt tggcgtcgta tcgttgcggc ccggcaggat
    13141 cgagtcattg ccgggtaccg acgctggcca tcgtgggagc caatccgacg ccggacgtgt
    13201 gcaagctggc caacttgtcg aacgtgacgg tgaccggttg ggtgccaagc gtgcgggatt
    13261 ggctggagcg agcccagacc gccgttgtgc cgatgctggg cggcacgggc atcaagaaca
    13321 agatcctgga ggcgtgtgcg gcgggctgtc cggtcgtggc gacctcaatc gcgaccggcg
    13381 gtctgccggt cggtgaagac ttcggcatca tcattgccga cggccccgag cgaattgccg
    13441 cggagttgct cagccttctg gttgacccga cgagggcccg gcggatcgga tcggccgggc
    13501 ttgatatggt gcgggcccgc ttctcctgga gccgtgtggc cgtggaattc cttgccgttc
    13561 tccggggaga gacggccgca ccggcggatt cggacggcgg cagtgacaaa caggatgaga
    13621 cttacaggcg atgcgaggtc gaagagatgg aggcgctcac tcatgcccct tcgtagcatc
    13681 gctttcttgc tctatttctt tggcagcgcg ggcgcctccc tcctcgtgcc catcgccggc
    13741 gtgttgtgct acatcgtgtt gtacaacgtc tatccgcagt cgacctggtg gggcaagtac
    13801 cttgaaccgc tgggcattcg ctacgcatac gttatcgccg cctgcctgac ggtcggggtg
    13861 attgtcaacc tcaatcgcct gcgttatgga cgacaggtcg cgcatccggt tgagtggggg
    13921 atgctgttgg tgtttctgag catgctcatg tcaggcgtga ccggtgtcgc ctggaacgag
    13981 cgtaccgatt tcgtgatcga caagatgtgg aaggtctttc tgtttgcctt catgatgagc
    14041 cacgtcgtgg tgacgcggcg gcacgtctgg ctgttgttga tcgtattcac ggtcatgtcc
    14101 ttgtacctcg gctatgaagc caagaatgcg ccgccggggg cttttctgca aaaccggctg
    14161 gacggtatcg ggggtcccga ctttcgcgag tcgacgggcc tggcgattca cctgctggct
    14221 ctgctgccgt ttacggctgt tttgctgtgg cagaagtact ggcgattcag ggtactggct
    14281 ttttttgccg cctgctacgg tgtcaatgcg atcctgctgt gtcgtgcgcg atcggcgttc
    14341 atcgccggcc tggtcgcggg tcttaccgcc gtctggtacg tgcctcgtcg ctatcgcagt
    14401 tgggccgcgt gtgtgctggt gcttggcctg ctgggcagcg tcaagctcag cgatcagtgg
    14461 ttctgggagc gaatgaaaac gatcgtgagc tcccgcgagg agcgcgaagt ctcgtcagca
    14521 gcccgcctgc ttatctgggc atcggcgtgg gaaatgatca aagacaggcc gctcggcgtg
    14581 ggcatcgggc aattccagcg gatgacgaat cgctatacca cgcccgaact gcggcggatt
    14641 tatggaaacc ccgccaccga atcggtgaac atgatcgccc gggacgccca caactcgttc
    14701 attctctgtg ccgccgagac gggcattccc ggtttggcct gttttctgtt gacgctgatc
    14761 atggcctggg gaacgctctc gcgattgaga cgacgggctc gccggtatct atcggacccg
    14821 gccatgtttg agatgctggt gttcgccaac cggatggcct tgctggttta cctggtcggc
    14881 ggcatgttta ccagccgctt ttacacggag ggtttctggt ggatggtcat cctgccggtt
    14941 tgtctgaagc gtgccgtcga gaacgagatt cgtgacgaag tgtgcgaaaa ggcgaccgtt
    15001 cgggcattgc tcgatgactg gattcctcga ggggaccggt tgcagcccga acttgtcatg
    15061 ggagggcaac cggcatgacg acgggcactt cggcgggacc gggccggcca acggatgccc
    15121 gcagtccgag cagcctgagg cgtattcgcg ttttgcacgt gattccctcg ttgttcagcg
    15181 gcgggatgga acgtttcctg atcgagttga tgagggcctc aacgccggtc ggcggggccg
    15241 gcgaaggttg ccgcgtgacc cacggggtgt gcattctgag agccgcggac gaccgcttgt
    15301 tctcgcagtg caagtcgctg gcgaccacct gggtgctcgg gaggcacaag gcgcgagact
    15361 ggagttgctg gaagcagata cggcaggtga ttcgcgattt cgagcccgac gtggtcgagg
    15421 cgttgagcac gggggcgtgg gtggacgcgg cccgagccgt cggccgctcg cgttcgacca
    15481 agctttcgct cgtgttccac gggcagatgg acacggtccc ggcgggtcgg atccgacgct
    15541 ggctgaaccg ttgggcggtg ggcagggcgg ccaccgtgat ttcggtttcg cgcgaagcgg
    15601 ccgatcggtt gatcagggaa tggcatatcc cggccgccaa gatggttgca gttcccaacg
    15661 gggttgacgt caaccgtttc catccggccg cttccgccga tgaacgcctg cgcgtccgcc
    15721 ggagactgga gatccccgac ggctcgaagg tggccatttg cgtggccaac ctcgtgccga
    15781 tcaaggcaat cgatgtcctg ctcgatgcct ggcggcaatt gtcggctgac cagcgcgatg
    15841 ctcgtctgtt gctggtcggg gacgggcccc tccgccggga gtggcacaac ctggcggaga
    15901 ccacggagtg tcacaagtcg gtgtccttcc tgggcaaccg cgatgatgta cccgcgttgc
    15961 tgcgggcttc ggatctgttc gtttcatcga gccgttacga ggcatgcagc atggcgattc
    16021 tcgaggccat ggccagcggg ctcgcggtgg tggcgaccga cgttggcggc aacggggagc
    16081 tggtagagcc tggtcgcacc ggctggctgg tgccgccgga ccgggccgac cttctggccc
    16141 aagcgcttgc ggcagcgttt gacgacgacc aagcccgtat acggttcggc cagacggcga
    16201 gaggagtcgt catggagcga cacgacctgg aagcttgcgc cagacggtat tccacgatct
    16261 atcattcgct ggctctaccc cacaccaagc ggccggcgac ggcaccggag gagcccgaat
    16321 gtgcggcata gccggcctgg tgcgatttgc cgggttgcgg ccccacgaga cgtccgcggg
    16381 cctcgatatg gctgcgttcc tgcgtcatcg cgggccggac gagatgggga cctacgtcga
    16441 cggccatgcc agtctggggc acgcgcggtt gagcattatc gacctggcgg acggccggca
    16501 gccgatgtcc aatgaggatg gaaccatcca ggtcgttttc aacggcgaga tctacaacca
    16561 cctggaattg gccgagcagc ttcgcgctcg cggccacgtt ttgcgaacgc gttgcgatac
    16621 cgaggtgctc gcacacttgt acgaagacca cggtgattcg ttccccgagt atctcaacgg
    16681 catgttcgcg atcgccatct gggactctcg ctcgcggcgg ctggtactgg ttcgcgatcg
    16741 actcggcgtc aagccgctct tttggaccga cgatggtcgc cgcgtcgcgt ttggatcgga
    16801 actgaaagcc gtactggcct gcggcgatct cgagcgaaga atcaatcctc tggcattggc
    16861 ggactacctc acgttcggcc acgtgccggc gcccaagacg attttcagcg gcgtgcgcaa
    16921 gctggaaccg ggctgtatgg ccgtctgcac cggctccggt acctcggttc accgctggtg
    16981 ggatattcca ttcggggatg ctgattccgc aaagcccgac gagcgagccg gacgacgctg
    17041 gacggaggac tttgcctcgc tgctggagaa tgcggtcgac atgcgactca tgtcggacgt
    17101 gcccctgggg gcatttctca gcggcggtgt tgattcatcg gccgtcgtcg ccgccatgtg
    17161 ccgctgctcc caagacccgg tactgactca cacggtcggg ttcgacgaac cttgctgtga
    17221 cgaacgggag gcggcccgcg tcgtggccca ccgactggat accgatcacc gcgaggtctt
    17281 ggttcgatcg gatgcggccg ccgccattga acggctgacc cgccattttg acgaaccttt
    17341 tgccgattcg tgtgccgtgc cgttgctgcg gctctcggag atcacgcggc agcgcgtcac
    17401 cgtcgcgctc tcgggagacg gaggcgacga gatgctggcc ggctaccggc gataccggtt
    17461 tgacctggcg gaagcgacga tccgcgagat gtggcctggt tggttgcgac gttccacggc
    17521 cggagcgatg ggccggatct accccaaggc cgactggttg ccccgctcgc tgcgggccaa
    17581 agcgacgttg tgcaacctgg cgtgtgatga tattaccgcg catttgcgga gcacttctct
    17641 ggcggccgga atgttgcccg atctgctgct gcggccggaa atgcggtgcc gcgtagtcgg
    17701 gtatgatccg tttgcgcgcg ggcgcgacct gttcgcccgt tgcccgtcgt cgctgctcaa
    17761 tcgtttgctt tacgttgaca tgaagaccct catggtcgac gacatcctga cgaaggttga
    17821 tcgggcgagc atggccgttg gtctcgaggt tcgcacgccg cttctggact atcgtatcgt
    17881 ggagctgtcc gcccggatgc ccgcctggct caagctggat ggtcggcgcg acaaggtggt
    17941 actccgcgac acggtccggc gctggctggg gcccgacgtc gccggtcgcc gcaagaaagg
    18001 cttcgacgtg ccggtcaacg aatggttccg ggggccgctg cggccgatgg caagcgacct
    18061 gctgatgtcc ccagaagcgg tctgttctca gtggatagac caggttgcgg ttcgcgcctt
    18121 gttcgcccga caccagagcg ggatcagcgc caacggtcat attctgtggg ctctgttgtc
    18181 gttggagctg tgggcgcggc agtacgcgcg cggacccggc ccggtgaggg aagggcaaga
    18241 cggtcgcatc ctgagacgcg gcacaggcgt tcgatcgccc gaggccgccg tttgcggggg
    18301 gatgccggcg tgaacatcct gaccacaaca ctgtgctatc ccacgcttgc ccggccggat
    18361 cagggcatct tcattcaacg tcgcgcggaa gcgcttgcgc agcatcctga cgtcagcctc
    18421 agcgtggttt cacctcagcc gtggtgtccg ctgcttcgct gcggcgaaca aaccattgct
    18481 cggaatgaac cgcttccggt aacttacccg cgcctggtca gcattccaat gatcggctgg
    18541 gcgaccgacg gtgtcgcgta cgccgggacg ttgctgcggg ttcttgcgcg acagaggcag
    18601 gccggcctgc ccccggtgga cctgatcgat gctcattttg tgtatcccga cggcgtgggc
    18661 gcctggctgg ccggtcggag gctgggaatt ccggtggtcg ttacggtgcg gggcaagatc
    18721 gttcggcttt ctcgccgcgc atttcgccgg atgcagatcg cggccatgct tcgcggcgtc
    18781 gatgcccgca ttgcggtcag tcgaagcctg gctgggtggg ttcacaaggt tgccggcagc
    18841 gacctgaatg ttgacgtggt ccccaacggc atacgaccgg acgtgtatcg cctggtcgac
    18901 cgtcagtggg ccaggatcgc tctcggctgg catcaaaccg ccaggtatgt tcttgccgtc
    18961 ggtcatttac agcgactcaa agggtttgat cggttgctcg tcgtcttgcc ggctctgcgc
    19021 gaggcgttgg gcgatgttcg tctggtactg gtgggcagtc gtcgtggcga gtggtggttt
    19081 gcccgaaagg ttctgcggat gatcgagcgg tgcaatcatg aagccggctg tcgatgtgtc
    19141 gagtttctcg gcttcgtcga tagtgaacgg ctcaacctgc tctacaacgc cgcggacgtc
    19201 atggtcaatt cgtcgcggat tgaaggctgg aacaactcga tctccgaggc cctcgcggcc
    19261 gggacaccgg tggtagccac ggacgtcggc ggcaaccctg agcagatctc ctccgacgag
    19321 ctcggcatga tcgtccggga cggcgatctc gatgctctcg ccggtgcctt gcgatccgct
    19381 cttgtcaggc cgtggaatcg agctctcatt gcgtcatcgg gcggggctcg cacgtgggcg
    19441 caggttgcag cagaggtaca cgcggtattc gagcgcgtgc tggcagcgcg ggcggcgccg
    19501 gcgggtcggc agtcgccggt cggcatcgac gcttcggctg cgatggcgga ggcgacgaca
    19561 tgaaccgctg gttcgccgga caggtcttct ggccgctgac cgagcggctt tgtcggcgcg
    19621 ataccatgcg ccggctggcg gaattgaatc gcacgcaaca tctgccgccg gaggtcatct
    19681 gcgagatcca ggagcgcaaa ctgcgccggt tgctttcgac cgcaagcgag cattgtccgt
    19741 tccatagccg gcgcattcgt caggcgggta tcgacgcgaa cgatccgcga cttggtctga
    19801 gcgctcttgg gttactgcct ctcttgacgc gagaggatat ccgggagcat cgcgacgaga
    19861 tgacctggtt cggctgccct cgcggcggac cgcgactcta taacaccggc ggttcgagcg
    19921 gcgagccgct caagttctat ttcgaccggt tccgccaggc ggccgatggg ggtgctcgct
    19981 ggcgggcacg acagtggtgg ggcgtgcagc caggtgaccc ggaaatcctc ctctggggcg
    20041 cgccgatcga gttggcggcc aacgatcgtc tgcgtcggtg gcgggatgcc ctgctgaatc
    20101 agtatgtgct caacgccttc gacatgacgg acacgaccat ggacgcgtac atcgatcgca
    20161 tccgtgcggt gcgtcccgtt tgtctgtacg gatatgccgg cagcctggcg cttctctcac
    20221 gtcatgcgct gaccaagggc ttggcgccgg gatcgttggg ttcgccacgt ttgcgagcgg
    20281 tcttcgtgac cggcgaggtg ctggtcgagc ctgatcgcga ggtgatcgcc tcggcgtttg
    20341 gtgctcccgt cgtcatcgaa tacggctgtc gtgacggcgg cttgttggcg ctcggttgcc
    20401 cggccggccg tttgcacatt ccacaggaga acgtcgtcat cgaactgctc gatgagcagg
    20461 gtgaacccat tgcggcgggg gacgtcggtg aggtggtagt gacacacctg gaaacccgtg
    20521 cgatgccgtt gatccgctat cgcaccggcg acctggcccg cgcatggccg gtttcggggg
    20581 gtcagtgcga atgtggcgta tcactggctg ccttggccga ggttcgcggc cgcctgaccg
    20641 atcagattgt ctgccgtgcc ggcggccaga ttcgccgcat gcacgctctt gccctgatct
    20701 acgtgctccg cgaggtcgac ggcatccgcc agttccggat tactcaacgg tcgcttgacg
    20761 aacttgacgt cgaggtggtt cccaccgccc gtttcagtgt cgactcggaa cgtgccatct
    20821 tgctggggct tcaccgccgg ctgggaagcg acgtcaggat ccgcctcaac cgccgggacc
    20881 acatccccgc gaccgcctcc ggaaagcacg cctgcgtgat ctcgcaggtc gggcaggaac
    20941 ttggacccgg gctgccgggt tgactggttc ctgcggctct tgcagccgcc gcccgcgcgg
    21001 tttgagccat cctgaaactc cacaagaaaa cgtgattcac caggaaagga agaagaaccg
    21061 cgggcgacca ccaaccgggc gtatcacggc cggcctgcct ttggcgggga aaccccatga
    21121 agagtgttga tcgcccaacc tggaccggtt gacatgcccg cccagactgt cacgcaccct
    21181 ctggtgccaa cccggcgcgg ttttatcttg atccgcgccc gaaatgcctt agactgaata
    21241 caaaggcgga gacaatcccg gtgcgggacc ggtatcttgc tggtcaacga ctggcaggaa
    21301 tctcgactcg cactgtgaaa gagtctgcgt tcccatttgg gtgaaacgtc tttccattga
    21361 cgagcccgac aggtccgttc cgttgctgtc acgcgtcggg gtaagggaat ggcatggcgt
    21421 gcatgtccga tcaccctgtg tgttcgcgaa tgccgtgtgg caatggttcc atcgcgggct
    21481 cagaatgccg gggcatggac gaaggttccg acgctgtgat gaagcggcga tacctttgca
    21541 taat
//
LOCUS       DAOTPW010000083        21250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2538_length_21250_cov_5.026657, whole genome shotgun sequence.
ACCESSION   DAOTPW010000083 DAOTPW010000000
VERSION     DAOTPW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2538_length_21250_cov_5.026657"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1393..2382)
                     /locus_tag="P5316_09345"
                     /pseudo
     CDS             complement(1393..2382)
                     /locus_tag="P5316_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2616..3836)
                     /locus_tag="P5316_09350"
     CDS             complement(2616..3836)
                     /locus_tag="P5316_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008842939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85204.1"
                     /translation="MNHKLLAAAIATVTAMNLTPPSASGATFGDDIAFLKRHTDLIVL
                     ADKPGLAKVALVPAWQGRVMTSTTGGDNGPSFGWINRELIASGKILPHMNAFGGEDRF
                     WMGPEGGQFSIFFAKGKKFVFEDWQTPAIFDTEPFKVLREADDKAVFGAEFSLTNYSG
                     TVFTVGVEREVELLTPEAAYERLGLKAAKDVSLVAYASNNKITNKGKEPWKKETGLLS
                     VWILGMFNPTPATTVVVPIKAGPESELGKKVTSDYFGEVPSDRLVVRDDAVFLRADGQ
                     YRSKIGISPRRSKAILGSYDADNKVLTIVQFNQPAGVTDYVNSLWKLQDDPYNGDAAN
                     SYNDGPPTLGAKPMGPFYELESSSPAAALGPGESLTHTHRTIHLTGPESALDRIAKAT
                     LGVSLEEIKTVFRK"
     gene            4069..4797
                     /locus_tag="P5316_09355"
     CDS             4069..4797
                     /locus_tag="P5316_09355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR85205.1"
                     /translation="MGDGAKVVLITGASSGMGAAAAIRLARDRAKVVLAARREDRLKQ
                     VAEQVRQAGGEALVCVTDVTRLADIRAMIARTMETYGRIDVLYNNAGLMRVAPIEQLS
                     HEDIEQMISVNVLGAVRVLKETVPIFKKQDSGLIINVSSVLGRKTRATAWVYAGTKWA
                     ITAINEGLREELAGTRIRVCSLQPGVVDTELFDAFEVHPAQWQKITHMVKPQDVAELI
                     AFIVSRPWYFNINEVLFRPTQQVV"
     gene            4958..6559
                     /locus_tag="P5316_09360"
     CDS             4958..6559
                     /locus_tag="P5316_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85206.1"
                     /translation="MRMHGFVFALVVVSGPAPAFAANQAVLWRDHKPQARLIQPQLGE
                     PVKDIVDITVNGALAEWCGWTLPKVTRADQPGLYIAVGDEKNNPVIGRLVQAGLKLDR
                     ADLGPEGFRILTHEAGDRRFIVITANSPVGLKHGCQELLFFRISITSEGASVDWPLDV
                     KMKPAFGYRGTYMLPCWSAYDSLENWKRVLKFHSELTLNRNWFWLAGFPLLEQYGGEY
                     QKSDLANGWNVNALVELCRAEGMKFYIGGGWFTWHHEKIADKSIDRGIQWYLDMIELL
                     PGTEGIYLEPPGEGREVDEKTWQERTEALARMAQTIWKKRPEFEFAIAIGKFNDPKYR
                     QAIHAIDDKRIYWWWCWGDPIMQNALTEHPLILRWHTTIQMSDYHGSTSPPEPREASL
                     TGFATSYDPGQGYGNPWNGWPAMGHDKPRNVDPRTMPFFSHQYRFRERCWNVGISEEA
                     FAARMARRLFDADMAADAIGQYLSLAAMCPKPLEADQKELAKITGFVDQNAGRGTPRN
                     RDTLARMREAIDGILATRAKESKPR"
     gene            6716..7429
                     /locus_tag="P5316_09365"
     CDS             6716..7429
                     /locus_tag="P5316_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85207.1"
                     /translation="MEASTGSLPEVGEDGLLADPVETAGKEEETMPDRAPRPLARWNK
                     RDQTLAKLQEGYEQVTRVIQEIQNHLVAQSDRSERICNSLEQLARSVNELPAIARTQT
                     QTLETIAAHIEATGSRTRQMAESLADVPKLAKAQSESLISINRQLEIAGEQRSLTCQT
                     LDRMGSALGTLSGSAQSQTERLKTIGDRTEQQVELLTEMISRQNRRFIMLFVVTIILA
                     GAALTAIAFLAWSAQRGQP"
     gene            complement(7470..8324)
                     /locus_tag="P5316_09370"
     CDS             complement(7470..8324)
                     /locus_tag="P5316_09370"
                     /inference="COORDINATES: protein motif:HMM:NF020229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR85208.1"
                     /translation="MSSAAVFWDRDGTLIEDPGYISSRDQVRLLPGAAEALKRVSAAG
                     FENIIATNQSGIARGFLDESILESVHQRLRDLLAAEGAAIDAIYYCPYLPGEEAVVEA
                     YRQDSDLRKPRPGMLLKASLERKIDLAASWSIGNSLRDAQAGRAAGCRTVVIEPDPVE
                     REKLRNHKDVDFVVSSLSEAVDAVLNCSRSSNDKSEAPLETGAGETLREILSFLRMVD
                     RRQQTVDVSLPAIVGMLFQVLAFVALIWAVFGLLNANNYSEPVLRILFTIALQLIAMT
                     FVLVSSKR"
     gene            complement(8450..9745)
                     /locus_tag="P5316_09375"
     CDS             complement(8450..9745)
                     /locus_tag="P5316_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRR85209.1"
                     /translation="MRLDNQPAKAENGAAWLRAGGSSKGANGGQTSGEILMRQSQIAC
                     VRVFPLSIPLRHAFSHAAHSRVHADPIIVQIELGGGTMGYGETLPRPYVTRENAESVL
                     TTIRGPLTAELLTFRPSSFAEALERIDALPFLDDEGRLICAARAGVELALLDVYSRFF
                     DRDIAEVAGWLGLPGLGSPGSARRVRYSGVLSGDDIVRLRRSVRKMRLFGLRDFKLKV
                     GYEDDVERVSAVANSLRRALRNGATLRLDANGSWSIERAIEICKALRSHPIACIEQPL
                     APGCEEQLIRLKNDTRSPIMHDESLVTQADAERLHRMGIADAFNIRLSKNGGFLPSLR
                     LAHWAGRHGIRHQLGCMVGETSVLSAAGRKYLECVPGVWFAEGSYGRFLLRGDVVKKP
                     IRFGFGGRFQTLSGPGWGVEVDPDLLRRYAAGAVAEIFL"
     gene            9820..10521
                     /locus_tag="P5316_09380"
     CDS             9820..10521
                     /locus_tag="P5316_09380"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HRR85210.1"
                     /translation="MEKAVVLASGGVNSTVAIAVTREQYEPHLFHINWGHRAAEREAI
                     AFQQTASAMGIENTMLVEMPALSTWGGNARASRRLAIEDAAALEKGTPATFAFALLPT
                     MLSAAASWAGSIGARRLVIGVSEDHGVPGPAISELYPDHRIEFIQTFNLMLKYAIPAG
                     QELVVEAPLLEMTRPEVVRLGMRLEVPFKLTWSCYANNDRPCDRCLACHNRAAGFLQA
                     KLPDPLVLSEKVVVL"
     gene            complement(10544..14173)
                     /locus_tag="P5316_09385"
     CDS             complement(10544..14173)
                     /locus_tag="P5316_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRR85211.1"
                     /translation="MSKTIAAKFLRNEGTDVFNGNELLVKGALETAGGVHLLTGYPGS
                     PVSAFFDVIAACASLFRERGIEGRISNNEAHSVAAVNGSQMAPLRAMAVMKSVGLHVA
                     SDALALGNLAGPHPHGGAVIVVGDDPWSESTQVPADSRFLFRHLFVPVIEPSTPQEVK
                     DYVGAAFELSRSSGLYLGYVITTMLADGGGTVTCGPNHYPLANTAHRIDLDTFSLDLE
                     NRVLLPPRTGRREVSFADRYERLAQAVRQLELNRFLDVPGSDELLLVTSGMAFQYVQQ
                     ALEDLGLDGRIPILKLAVTWPLDRREIEPLLRRFGHVVVVEERRAFIEEQLSQLALGL
                     AQNGAGRVASVWGKLFPGPSPGFRTTLGLNVSMVTERLGSLLRAIESPFAAEASRRIE
                     RELRFHSQTVREKLNIPPRTPTFCPGCPHRDSANVLLQIKEQFNDEAYMRKHHGCGAI
                     DLVFHGDTGCYTMLMFEPNAPLMHNYSGMGLGGATGSGIDPFIRNKQVVFMGDSTFFH
                     SGATAISNSIANRQDITYVILANGTTAMTGHQPLPTAETDLLQRPVPVQQIDAVVRGF
                     TGGRCTVIRTDPGDRDNYKELLERTILADGVKVVIADKECGITAQRRRMKLERAEQNR
                     KGFVRRKVYMNVTPEVCEYCLECTSRTGCPGLTIEPTDYGPKVATDLSWCVNDGACAK
                     LAVCPAFEEVVVTRRKPPTPRGHKIMLADIPEPVPQFKGDVWRAWIAGVGGMGIGTAT
                     AVLVHAAQHEGYSVQFSDKKGLAIRNGGVYSQLTLSRNGRTAGMTLGYGKADLLLGMD
                     LLEAVRAIDPEAPFQVASPERTAVVVNCDTAPTIRTLLGRDAAAADEWEAALRRAARP
                     GQYFSARVAALCERIFGTKLYVNLTMLGIACQRGLIPLSLEAIQAGIRKTITVDVEKN
                     LRAFEVGRKLVSHPELFGEIFGDTEDGRSLARTVRAKAAYLNMRQMGSRRALRTEQLG
                     SGAKRRLADTPLARTYKHMVYTTLRACRELDRRTMRDIAVRIYDLIQWGGVRYARRYV
                     QRIRRVFLADHEQFEFSATRAVVHNLARLMLIKDEFYVAYLLTSYEKLRRDRQRFNVN
                     PAIGDRIRYRRVFHPRIMGRRVDIRLPHWCLYAMRSLRFLRKPMFWYRLRERRFLRWY
                     EEIVDGFCSADESRYEQYVELLRLPDTVRGYAEIRWPRMKQARDRAAQILARSVSSSI
                     EAKSV"
     gene            14655..15908
                     /locus_tag="P5316_09390"
     CDS             14655..15908
                     /locus_tag="P5316_09390"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR85212.1"
                     /translation="MNQADNPIQRPALHYAWIVLAVGTLVVVGSLGLARFGYTMLLPS
                     MQTALTMDNTKAGGLATANLAAYLALAVTGGVLASRYGPRIVITIGLSVAALGMALTG
                     SAASFGEAMIWRVLTGLGSGASNVPVMALLSAWFAQRRRGLAAGIGVTGSSLALIVLG
                     PLVPRVLAADEENGWRICWFLFAAVTFGLAVLSWAVLRNRPADIGLHPLGAAEDDEPG
                     VAAQGGLRWGRVYGSGVVWHVGLIYIAFGFSYMIYMTFFTKYLIAEGGYTKEAAGALF
                     MLVGWVSLPCGLILGWISDIIGRKGALVIVYLIHAIAFALFALWPSPAGFTISAILFG
                     LSPWTIPAIMAAICGDVLGPRLAPAGLGFITLFFGIGQAAGPIAAGALADADANKSLS
                     SAFLLAAGVALLGAIGSLLLRRPKG"
     gene            16247..17737
                     /locus_tag="P5316_09395"
     CDS             16247..17737
                     /locus_tag="P5316_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85213.1"
                     /translation="MSQKTNRRDFLKSSALAGAGFWVATRSARAQSKSPNEKLNIACI
                     GTGGMGSGDTDSVASQNIVALCDVRPSALESKLDKFPDAKGYADYRKMFDEMGDKIDA
                     VTISTPDHTHAHAAMMAMKLGIHCFVQKPLCHDVWEARELTRMAKELKAKGGGKLCTQ
                     MGNQGTSSAGLRKAVEVVWSGMLGAVREVHVWTNRPVWPQGTGAVLDLVDVRYAMHGD
                     AGLRVVKKETGEKEKPHPEPHDVDWDLWIGTAPYRPYQPQYQPFNWRGWWDWGTGALG
                     DMACHTANMAFMACKLGYPTRVEAEMSEYNPETFPMWSVIRYEFPARGNMPPLKWTWY
                     DGGNDKPAWVNKKLKELAHGRDIPGSGSILIGEKATLFSPNDYGEDWVLLPEGQKRDE
                     VKVDQILPRSPGHKEEWFNAIRENKPELAMSNFSYAGPLTETVVLGCVAMRVAPGMEV
                     EVEQPDGKKKKVSHKHIEWDGPNMKITNIPAANAFLKREYRKGWEL"
     gene            17998..21117
                     /locus_tag="P5316_09400"
     CDS             17998..21117
                     /locus_tag="P5316_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRR85214.1"
                     /translation="MAKKLCSAWISGVNMMVYLAAGAAASDTQTINVCFVRQGALGAV
                     HRTASPSTPAIQTAILELVAGPTAQEGAAGFTSWIPSGTKVVNIRLSDDGVIVDLSPE
                     VLQDLDESTLLGIFDQFRATLWAFPDVLSIHLTCQGQLLASYLPPAPEIESMPETMSQ
                     PMRLSMMEPEPEQSLLNGTALSGRNITIGPSHGRYWHDDYGWIWQRGDPCGFGEAVLE
                     DTNSIRLCQFLYQYLVQDGATVHVPREMNESNCCHSATGLPWWKMAARYWLQANGLPS
                     SIWDSSTTDYNDDIRARPLFADYRGSDIYIAHHTNAFDGTASGTVTYRDTAMEHPEHE
                     ANSYILAQRVQSNVCDAIKNIYGIADWYNRGVLDAKGGFGEIRIPNRPACLIELAFHD
                     NCTRDALYLTDNFFRSVAEWGLYKGVCQYFGASPTWDCYSDEYVSDTIPAIMNPSQSY
                     GVSITFRNRGVVWSEARSFRLGAVGDSDPFTAFNRVNISGEVGPGQTYTFSFTMTAPA
                     APGTYITDWRMVRDGVTWFGATCSKQVEVGTSGPQPPSIVQHPADQTVPLGTVAAFTV
                     QATGDSPLYYQWQKDTVDLSDGGKISGANSATLQLTGVDVPDQGVYRCVVTNALGSAT
                     SNAATLTVTAPIFMVESRSGGQNYAKYSETGGWANSGGKSTAAGVTAGIGSRYGSTYR
                     SVAGEKHAIFGADLPAAGTYEVSVTWGANANRRTPILHRIVHAGGTTDVNVDQAATAN
                     TWVSLGVYTFGAGSDVGSVDVNNLNIDASGSMYADAVKWVYVASPPVIDQHPAVQSVC
                     PGATATFTVAASGGGTLAYRWQKNGVNLTNGGHYSDVTTATLKVLSADVADVANYRCV
                     VTNISGSATSNEAGLTLKAATSITQHPESQTVCPGSATGVQFAVVATGDGTVSYEWQK
                     NSVKLTEGGHYSGVTSPALTVLNVDSSDIGTYRCLVTAGCGTATSNEAVLALRPMVTA
                     DFDEDCDVDQADYDLFAACSSGPGVPTGTGCNGKDLDGDGDVDQADFGVFQRCYSGEN
                     LPPDADCAN"
BASE COUNT         4310 a         6547 c         6510 g         3883 t
ORIGIN      
        1 ccgtcagggc ctccggattg aaggatcccg ctgcaatctc cggcaatcgc gagatgtatt
       61 cgcagattcg ggcaacacgg gcactatgct cccagacgat cgggtctggt gccgctttct
      121 caactgctac gcacagatct cgctcagccc gttgccagag atggtccgcc atggcccctc
      181 caggccctga ctcgcccaga atcaccgacg ggagtcgcag gataccagcg cggcaggagt
      241 cactgcgccg ccccggagac ccttggcccg gcagccgacg ctcttggccg tcgggcccgg
      301 ccccccgggt gcagaatggc atcagggccg caaatgccgc aacgttcgct cttacattat
      361 cttcaagagc gactccattg ccagtctttt ttgagcagaa ttttaatggc ggagtcgtat
      421 ttgcgttgga caagcgcggc cggcaaacgc actttggggg tgttcctttg tatctggtca
      481 atgtcctcct gaagcgccca ccccatgcag cgcttccgcc cggctcagtt tgggccagcc
      541 gtagccgtag atcgcgatca gaaggaaaca gaccaagggc acgatgaaac cgcgcgacat
      601 gtcgtagtgg tcggcaacgg cccccatcag cttcggcagc agtgcgccgc ccacaatggc
      661 catgacgatg taggccgacg cgcctttcgc cttactgcct aagccgtaga tgcccagggc
      721 aaagatggtc gggaacataa tggacatgaa gaaatagctt acgaagacgc tcaccactga
      781 aagccagccg agcttgcaga agaccaccag gcaggcgata acgttcagca ctgcgtagag
      841 gcccacgatt ctgtgcgcgg aaacccgccg cagcagcgca gcccccacga ctcgtcccac
      901 gaggaagcaa atgaacccga atgacgccaa gttggctgcg aactggttcg taaagcccac
      961 aacgccgtcc tgataggcca gttccttcgg atacgcatcg gccagcaaaa gccggttgag
     1021 ccgcagcctg ttctgaccct tgggatcctg ggccagcagt ttcctcgcct cgtcacgcag
     1081 ttcgattcct ttgaatcgtt cgggctcgta aaggttttcc ttctggatca gattattcag
     1141 gtcctgcatc atggcaacgc gggccgccgt ctcgcccacg gtacggttct tgaattcggc
     1201 caaggtttgg cgggtcgaat cagagagctt ggcggccagg taggacgaca gtggatcctc
     1261 ttctcccggc gaggtcagct tctcggcaag ggatggcacg tccttgaaat cggtcccgag
     1321 gaatgccgtt ctgagcccta cccacttgct atcctctttc agccaggagg ccggcagtga
     1381 gggcggctca gaagtgacgt aattaatcag aaagctgaag atgcccgcct gagcggccac
     1441 gtagaaaaac tgggccagta cggcacccgt gaagtgctcg tgcgaccaga tgctgtgatg
     1501 cgatagccgc ttggttgcgc cgacaagcca gatcgccgca atcacaactg caaggcagcc
     1561 gaaggcgaaa agcgtcaaca gcagggcggt atcggcggtg atgacgaaac gggatgtgag
     1621 cggcagcgcc gagacaaact tcgcaaggcg cggaccaaga cccatggaac cgagggccag
     1681 ccagagaatc atgccaaaca cgcccaacaa tgcacaaatg ttgcccagca acagaacgaa
     1741 cgacaacccg cggttcacct ttcggatgac cggcgctttg cctccggcgt cactgttttc
     1801 atcaatcgca tagtcatctt tggccttgat gtccggcacc ggtgcgaaga aaaagaccac
     1861 ggccagcagc aagaccacca ccgccacgcc gacgtaagga atccatagcg tcctagaacc
     1921 cgtgctccgc ccgaattcat ccgtgccata aaagaataat ccgccagcga tcggtcccag
     1981 cacccaaccg acgccgttgc acgattgggc caggttgatg cgcgtggcgg cataacgcgg
     2041 cggtcccagc acggtggtat aggggttggc gaccgtctcg agaaacgtca ggccggaggc
     2101 cacgacgcac acgccgagaa gaaaggctgc gaaggccatg ttctgcgaga ccgccgccac
     2161 gtccgacgtg gccaaggccg cgattttcgt cgctggcaga aaccagaagc cgcccacagc
     2221 caccagcagg agcccagcga tgatgccgcc cttgtacccc aggcgcgtcg ccagccaacc
     2281 tgctggaatc gacatgagga agtagcccat ccagtgcgcg aactgcaccc aggccgattg
     2341 cgacaggctc aggcccagtt cctcctggaa gtgcttgtcc atcacgtcaa tcataccgtt
     2401 gcagaatccc cacaggagga acaacgaact gacgagacag aacgtgagaa gcacgtttct
     2461 gccgtcgggc aggacgaaca tgctttgctt ttcggccatc gcgttgaggg ctggctgggt
     2521 ctgagtactc atggtattcg ggtctctctg gatacggatc ggccgccacg catctgttgt
     2581 cggccggaat tcacatcaca cactgccaag ctggcttatt tcctgaacac agtcttgatt
     2641 tcctcaaggg agacccccag cgtcgccttg gcgatcctgt cgagcgcgga ttccggtccg
     2701 gtgaggtgga ttgtgcggtg cgtgtgagtc aggctctcgc ccggccccag cgccgcggcc
     2761 ggggaggaag attcgagctc gtaaaatggt cccatcggct tggcacccag ggtaggtggt
     2821 ccgtcattgt agctgttggc tgcgtcaccg ttgtagggat cgtcctgcag cttccagagc
     2881 gagttcacgt aatccgtgac gcccgccggc tggttgaact gcacgatcgt gagaaccttg
     2941 ttgtccgcgt cgtagctgcc gagaatcgcc ttgctgcgcc gcgggctgat accgatcttg
     3001 ctcctgtatt gaccgtctgc acgcaggaat accgcgtcat ctcgcaccac cagacgatcc
     3061 gacggcacct cgccgaagta atccgaagtg accttcttgc ccagttccga ctccggtccg
     3121 gctttgatag gtactacgac cgtcgtggcc ggcgttggat tgaacatacc gaggatccag
     3181 acggacagca gtcccgtttc cttcttccag ggttccttgc ctttgttggt gatcttgttg
     3241 ttcgaggcat aggccacaag actcacgtcc ttggcggcct tcaatccgag tctctcatag
     3301 gctgcctccg gcgtgagcag ctccacctcc cgctcgacgc ccacggtgaa aaccgtgccg
     3361 gagtagttcg tcagcgaaaa ctcggcgccg aagaccgcct tgtcgtccgc ctcgcggaga
     3421 accttgaagg gttccgtgtc gaagatggcc ggtgtttgcc agtcctcgaa tacgaacttc
     3481 ttgcctttcg caaaaaagat agagaattgc ccgccttccg gccccatcca gaagcggtct
     3541 tcgccgccga aagcattcat atggggaaga atcttccccg aagcgatcaa ctcgcggttg
     3601 atccatccga aactggggcc gttatcaccg cccgtcgtgc tggtcatcac gcgtccctgc
     3661 caggccggca cgagagcgac tttggcgaga cccggcttgt cggccaggac gatcaagtcg
     3721 gtgtgccttt tcaggaacgc aatgtcatcg ccaaaagtgg cgccggatgc ggagggtgga
     3781 gtcaaattca tcgctgtaac agtggcaatt gctgcggcca gaagtttgtg gttcatagac
     3841 atttcacatt tcatagtgca acggttacgc ttgacgctgt gtgcaagcga ccgcggaagt
     3901 ctaccacgct cgatccccct catcaagtcc tcgccgaagg gttcagcgtt gggggttcgg
     3961 ggttcagagg agggggtgtt tcaacagagc gggcaggccg gatcacgccg ggttcaatgt
     4021 gggcgggcag tggcccgccg agctgatgga acagaggagg agtgattcat gggcgatggc
     4081 gcaaaggtcg tattgatcac cggggcgagt agcggcatgg gagcggcggc ggccatccgg
     4141 ctggcgcgtg accgggcgaa ggtggttctg gctgcccggc gcgaagatcg attgaagcaa
     4201 gtagccgagc aggtccgcca agcgggcggc gaggctcttg tctgtgtcac ggacgtcacc
     4261 cggctcgcgg atatccgggc aatgattgcc cgaacgatgg agacctacgg acgcatcgac
     4321 gttctctaca acaacgccgg gctcatgcgg gtcgcaccca tagagcagct ttctcacgaa
     4381 gacatcgagc agatgattag cgtcaatgtc ctgggagccg ttcgggtgct caaagagacc
     4441 gttccgatct ttaagaaaca ggacagcggg ctgattatca acgtttcctc agtgctcggc
     4501 cgcaagaccc gggccacagc ctgggtctac gccggaacga agtgggcgat cacggcgatc
     4561 aacgagggtc tccgcgagga actggccggt acaaggatcc gcgtatgttc gctgcagccc
     4621 ggcgtcgtgg acacggaact cttcgacgcg ttcgaggttc acccggccca gtggcagaaa
     4681 atcacacaca tggtcaagcc ccaggacgtt gcggagctca tcgccttcat cgtcagccga
     4741 ccgtggtact tcaatatcaa cgaggtgctc ttccggccga cacagcaggt cgtttagcgg
     4801 gaatctgccg agtggacagg ggcgtcgaaa acgggcacaa tcctgaggat ttgctgtcgg
     4861 ttgccaaggc gtcaagccgt gaggatacgc ggagcttggc cgccgacagt gaatgattcc
     4921 cgagaacccg agcggccata tggccaggag gcacgaaatg aggatgcacg gtttcgtttt
     4981 cgcactggtc gttgtcagtg gcccggcacc cgcgtttgct gccaaccagg cggttctttg
     5041 gcgcgaccac aagccccaag ctcgcttgat tcagccgcag cttggtgaac cggtcaagga
     5101 cattgtggac atcaccgtca atggcgcgct cgccgaatgg tgcggctgga cgctgcccaa
     5161 ggttacacgc gccgaccagc ccggccttta catcgccgtc ggtgacgaga agaacaaccc
     5221 ggtcatcggc aggctggtcc aggcggggct caagctggat cgtgccgacc tgggccccga
     5281 aggtttccga attctgacgc atgaggccgg ggaccgtcgg ttcatcgtga tcaccgccaa
     5341 tagccccgtc gggcttaagc acggctgcca agaacttctg ttcttccgca tcagcatcac
     5401 gtcagaagga gcctcagtcg attggccact tgacgtgaag atgaagcccg ctttcggcta
     5461 tcgcggtact tacatgttgc cttgctggtc ggcgtacgat tctctggaga actggaaacg
     5521 tgtcctgaag ttccactccg agctgacgct caatcgcaac tggttctggc tggcggggtt
     5581 tccgctgctg gagcagtacg ggggcgagta ccagaaaagc gacttggcca acggctggaa
     5641 cgtcaatgcc ctggtcgagc tgtgccgggc ggagggtatg aagttctaca tcggaggcgg
     5701 ttggttcacc tggcatcatg aaaagatcgc cgacaaatcg atcgaccgcg ggatccagtg
     5761 gtatctggac atgattgagc tcttgcccgg aacagagggt atctatcttg aaccccccgg
     5821 cgagggacgc gaagttgacg agaagacctg gcaggagagg acagaggcat tggccaggat
     5881 ggcccagacc atctggaaaa agcggcccga attcgagttt gccattgcca tcggaaagtt
     5941 caatgatccg aaataccggc aagcgattca tgccattgac gacaagcgga tctactggtg
     6001 gtggtgctgg ggcgatccga tcatgcagaa cgcgttgacg gaacaccctc tcatcctccg
     6061 gtggcacacc acgatacaga tgagcgacta ccacggatcc acgtcgccgc cggagccgcg
     6121 ggaggcgtca cttacgggat ttgcgacaag ttatgacccc ggccagggat acggcaatcc
     6181 ttggaacggc tggccggcca tggggcacga caaacctcga aacgtcgacc cacgaacaat
     6241 gcccttcttc tcccatcagt accggttcag ggaacggtgc tggaacgtcg gaatctcaga
     6301 ggaggctttt gcggcgcgca tggcccgccg gctgtttgat gccgacatgg ccgccgacgc
     6361 catcggccag tacctcagcc tggccgcaat gtgcccgaaa ccgcttgagg ccgaccagaa
     6421 ggagcttgcc aagatcaccg gtttcgtcga tcagaacgcg gggcgcggca caccccgcaa
     6481 cagagacacg cttgcccgga tgagggaggc gatcgacgga atcctcgcga cccgagccaa
     6541 ggaaagcaag cccagataat cgcttgcctc ggaaggccaa tgtcgttaga aacacggtgg
     6601 agacgtttcg tctttacgcc gatcagcacg cgaccgctgg cgggagggct ccacaatgat
     6661 gaaaaccggc tcatggtggc gacgtctgcg aattccgtgg aggaggcatg aaacaatgga
     6721 agcttcgacc ggaagtctgc ccgaggtagg cgaagacggg ctcctcgccg accctgtcga
     6781 gacggccggc aaggaagaag aaaccatgcc cgacagggca ccgcggccgc tggctcgctg
     6841 gaacaagcgg gaccagacgc tcgctaagtt gcaggaaggt tacgagcagg tcacgcgagt
     6901 gattcaggag attcagaacc acctggtcgc ccagtccgac cgaagcgagc gaatctgcaa
     6961 ttcgctcgag cagttggcac gttcggtcaa cgagttgccg gctattgctc ggacacaaac
     7021 ccagacgctt gagacaatcg ccgcccatat cgaggcaaca ggcagccgca cacgacagat
     7081 ggccgaatcg ctcgccgatg tgcccaagct ggcgaaggcc cagagcgaat cgctgatcag
     7141 catcaaccgg caactggaga tcgcgggcga gcagcgctcg ctaacctgcc agacgctcga
     7201 ccggatgggc tcagccttgg gaacactgag cggttccgcc cagagccaga ccgaacgtct
     7261 caaaacgatc ggcgaccgaa ccgagcaaca ggtggagctg ctgacagaaa tgatctctcg
     7321 gcagaacagg cggttcatca tgctgtttgt ggtgacgatc attctggcag gtgcggcctt
     7381 gacggccata gcatttctgg cgtggtccgc gcagcgtggc cagccttgac aaggccgctc
     7441 tgccggcgtc atcgccaccc ccctgccggt catcgcttgg aggaaacaag gacaaacgtc
     7501 atcgcgatga gctgaagtgc gatggtgaaa aggatgcgca ggaccggctc actgtagttg
     7561 tttgcgttca acaggccgaa caccgcccag atcagggcca caaacgccag cacctggaac
     7621 agcatgccca cgatcgccgg caacgacacg tcgaccgtct gttgtcgccg atcgaccatc
     7681 cgaaggaagc tgagaatctc ccgaagcgtc tccccggcgc cggtctccag cggggcctcg
     7741 gacttgtcgt tcgacgatcg cgagcagttg agcacggcgt ccaccgcctc ggacagggaa
     7801 ctcactacga aatcgacgtc tttgtgattt cgtagttttt ctctctcaac gggatcgggc
     7861 tcaatgacca cggttcgaca gccggccgca cggcccgcct gcgcatcgcg cagcgagttg
     7921 ccgatgctcc acgaagccgc caagtcgatc tttcgttcca atgaggcttt gagaagcatg
     7981 cccggtcgcg gcttgcgcag gtcgctgtcc tgacggtacg cttcgaccac ggcctcctcg
     8041 cccggcaggt agggacagta gtagatggca tcgatagcag ccccttcggc cgccagaaga
     8101 tcccgcaacc gctgatggac gctctccagg atcgactcat ccaggaagcc gcgggcgata
     8161 ccggactggt tggtggcaat gatgttctcg aaaccggcgg cggagacacg tttaagagct
     8221 tcagcagccc cgggcagcaa ccgaacctgg tcacggctgc tgatataacc cgggtcttcg
     8281 atcagagttc cgtcccgatc ccagaagact gctgcagatg acatcgtgcg tcctcacctg
     8341 tttcggaagg ctcggaagac agtagcctag ccgaggaaga ggctcagagc aaaccgtcct
     8401 cggaaccgat ctggtcccgg gcgggcgcag ctcggcttga caagggccgc tagagaaaga
     8461 tctcggcaac ggctccggcg gcatatcgtc gcaacaagtc tggatcgacc tcgacgcccc
     8521 atcccggtcc tgacaaggtc tggaatcgtc cgccaaagcc gaagcggatc ggcttcttga
     8581 cgacgtctcc acgcagcaga aaccgcccgt agctgccttc agcgaaccac acacccggca
     8641 cgcactcgag gtatttccgg ccggcggccg acaaaacgct tgtctcgccc accatgcatc
     8701 ccagttgatg tcgaatccca tgcctgcccg cccagtgagc caaccgcaac gagggcagaa
     8761 accctccgtt cttgctgagc cgaatgttga acgcgtcggc gatgcccatg cggtgcagtc
     8821 gctccgcgtc ggcctgcgtc acgagcgact cgtcgtgcat gatgggtgac cgggtatcgt
     8881 tcttcagccg aatgagctgc tcctcgcatc ccggcgcgag aggctgttcg atgcaggcaa
     8941 tggggtgact ccgcaacgcc ttgcaaatct cgatcgcccg ttcgatcgac caggaaccat
     9001 tggcatccag ccgaagcgtt gccccgttcc tcagggcccg gcgcagggag ttcgcgaccg
     9061 ccgatacccg ctccacatcg tcctcatagc cgaccttgag cttgaaatcc cgcaaaccga
     9121 acaagcgcat cttgcgaacc gatcgacgca accggacgat gtcgtcaccg gagaggacgc
     9181 cgctgtagcg gacacgacgc gcacttccgg ggctccccag accgggcagc cccagccagc
     9241 cggccacttc agcgatgtcc cggtcgaaga atcggctgta gacatccaga agggccaact
     9301 cgacgcctgc tcgagcggcg catatcagcc gtccctcgtc atcgaggaac ggcagcgcgt
     9361 cgatacgctc aagtgcctct gcgaaacttg agggacggaa ggtaagcagc tcggcggtca
     9421 gcggcccgcg gatcgtcgtc aggacggatt ccgcgttctc tcgtgtcaca tagggtcgag
     9481 gcaacgtctc gccatatccc atcgttccgc cgcccagctc gatttgcact atgatagggt
     9541 cggcgtgaac gcggctgtgc gcggcatggc tgaaagcatg ccgcaaggga atggataacg
     9601 ggaaaacacg aacgcaggca atctgcgact ggcgcatcag gatttcgccg ctggtctggc
     9661 ctccgtttgc ccctttcgac gaacccccgg cacggagcca cgccgcacca ttctcagcct
     9721 tggccggctg attgtccaga cgcaaggtcc gagaacccct cggctgctga tgaagcctct
     9781 tgcgttggcc gatgaaacct gaccgggagg atttcgcgca tggagaaggc cgttgtgctc
     9841 gccagcgggg gtgtcaacag cacggtggcc atcgcggtca cgcgtgaaca gtatgagcct
     9901 caccttttcc acatcaactg ggggcatcga gctgccgagc gcgaagcgat tgcctttcag
     9961 cagaccgcca gcgccatggg gattgagaac acgatgctgg tcgagatgcc cgccctgtcc
    10021 acttggggcg gaaatgctcg cgccagcagg cgtctggcca ttgaggatgc agccgccctg
    10081 gagaaaggca caccggccac cttcgcgttt gctctactcc cgaccatgtt gagcgcggcg
    10141 gcgtcctggg ccggttcaat cggtgcccgc cgccttgtga tcggcgtcag tgaagatcac
    10201 ggtgtccccg gtccggcgat ttccgagctc tatcccgatc atcgcattga gttcattcag
    10261 accttcaacc tgatgctcaa gtacgccatc ccggccggtc aggaactggt tgtcgaggcc
    10321 cccctgcttg agatgacgcg cccggaagtc gttcgactgg gcatgcggct cgaggttccc
    10381 ttcaaactga cctggagctg ctacgccaat aatgacaggc cctgtgaccg atgcctggcc
    10441 tgccacaacc gggcggcggg attcctgcag gccaagcttc ctgatccgct ggttctctcc
    10501 gagaaggtgg tggtgctctg agagaggaac gaaagcccga tcctcaaacg gacttcgcct
    10561 cgatcgagct ggaaacggat ctggcaagga tctgggcggc gcggtcacgg gcctgcttca
    10621 tccttggcca gcgaatctcg gcataaccgc ggaccgtgtc cggtaaccga agaagctcca
    10681 cgtactgctc ataacgcgac tcatccgccg agcagaagcc gtcgacgatc tcctcgtacc
    10741 aacgcaggaa ccggcgctcg cggaggcgat accaaaacat cggcttgcgc aggaagcgca
    10801 gacttcgcat tgcgtacaag caccagtgcg gcaggcgaat atcgacacgg cggcccatga
    10861 ttctcgggtg aaacactcgc cgatagcgga tccgatcgcc aatggccggg ttgacgttga
    10921 atcgttgccg gtcgcggcgg agcttctcat aactggtcaa cagataagca acgtagaact
    10981 cgtccttgat cagcatcagt cgggccagat tgtgaacaac ggcccgcgtc gcagagaatt
    11041 cgaactgctc gtggtcggcc aggaagaccc gacggattcg ctggacgtag cggcgggcgt
    11101 atcggacccc accccattgg atcaaatcat agatgcgcac cgcgatgtcg cgcatcgttc
    11161 tccgatccag ttcgcggcag gcccgtagtg tcgtatagac catgtgtttg taagtccggg
    11221 ccaggggcgt gtcggccaat cgccgtttcg cgccgcttcc gagttgctcg gtccgcagcg
    11281 ctcggcgcga tcccatctgt cgcatattga gataagcggc cttggctcga acggtgcgag
    11341 ccagcgatcg gccgtcctcg gtatcgccga aaatctcgcc gaaaagctcg ggatgactga
    11401 ccagtttgcg tccgacctcg aaggccctca ggttcttctc gacgtccacc gtgatcgttt
    11461 tgcgaatgcc ggcctgaata gcctccagcg acaaggggat caagcctcgt tgacaggcaa
    11521 tccccagcat ggtcaggttg acgtagagtt tcgtgccgaa gattcgttcg cagagcgcag
    11581 ccacccgtgc tgaaaagtac tggcccggcc gggcagcacg ccgcagcgcg gcctcccatt
    11641 catccgccgc ggcggcatca cggcccaaca gcgtgcgaat tgtcggcgca gtgtcacaat
    11701 tcaccaccac tgccgtgcgt tcgggcgaag cgacctggaa cggcgcctcc gggtcgatcg
    11761 ctctgaccgc ctcaaggaga tccatcccca gcagcagatc cgccttgccg tatccgaggg
    11821 tcatgccggc cgtccggccg ttgcggctca gggtcagctg cgaatagacg ccgccgtttc
    11881 ggatggccag acccttcttg tcgctgaact gcacgctgta accctcgtgc tgagctgcat
    11941 gtacgaggac ggccgtggcc gtgccgatgc ccatgccgcc gacgccggcg atccaggctc
    12001 gccagacgtc acccttgaac tgggggaccg gctcgggaat atcggccagc atgatcttgt
    12061 gcccgcgagg ggtcggcggc ttgcggcggg tcacgacgac ctcctcaaac gcaggacata
    12121 cagccagctt ggcgcacgcg ccatcattga cgcaccatga cagatcagtc gcgactttcg
    12181 gaccgtaatc cgtgggttcg atggtcagcc cggggcagcc cgtcctgctc gtacattcga
    12241 ggcagtattc gcagacttcc ggcgtgacat tcatgtacac cttccggcgg acaaagccct
    12301 tgcggttctg ctcggcccgc tccagcttca tccggcggcg ctgcgcggtg atcccgcatt
    12361 ccttgtcggc gatgacgacc ttgacgccgt cggccagaat ggtccgttcg agcagctctt
    12421 tgtagttgtc gcgatccccg gggtcggtac gaatcaccgt acaacgcccc ccggtaaagc
    12481 cgcggaccac ggcgtctatc tgctggacgg ggaccggacg ctgcagcagg tcggtttccg
    12541 cggtgggcag aggctggtga ccggtcatgg ccgtcgtacc gttggccagg atgacgtagg
    12601 tgatgtcctg ccggttggca atcgagttgc tgatcgcggt tgccccggaa tggaaaaagg
    12661 tcgagtcgcc catgaacacg acctgcttgt tgcggataaa gggatcaatg ccgctgccgg
    12721 tcgcaccgcc gaggcccatg ccagagtaat tgtgcatcag cggagcgttg ggctcgaaca
    12781 tcagcatcgt gtagcagccg gtgtcgccgt ggaaaaccag gtcgatcgcc ccgcagccgt
    12841 gatgctttcg catgtaggcc tcgtcgttga actgctcctt gatctgcagc aggacgttgg
    12901 ccgagtcgcg gtggggacaa cccggacaga acgtcggcgt gcgaggcggg atgttgagtt
    12961 tttccctgac cgtctggctg tggaaacgca actcccgctc gattcgcctg gatgcctctg
    13021 cggcgaacgg tgactcgatc gccctcagca ggcttcccag tcgttcggtg accatcgaga
    13081 cattaagccc caacgtcgtc cgaaaacccg gacttggacc agggaagagc ttgccccaaa
    13141 cacttgccac cctgcccgcc ccattctggg cgaggccgag agccaactgg gaaagctgtt
    13201 cctcgatgaa cgcccgtcgc tcttcaacga cgaccacgtg gccgaatcgc cgcagaagcg
    13261 gctcgatctc ccgccgatcg agaggccaag tgacggccag tttgagaatc gggatccgcc
    13321 catcgaggcc cagatcctcg agcgcctgtt gcacgtattg aaacgccatg ccgctggtga
    13381 caaggagcaa ctcgtcgcta ccgggcacgt ccaagaaccg gttcagctcc agttgccgaa
    13441 ccgcctgagc aagccgttca taccgatccg cgaaagagac ttcccgacgg ccggtccgcg
    13501 gtggcagcag aactcggttt tccaagtcca acgaaaaggt gtcgagatcg atccgatgcg
    13561 ccgtgttggc cagcggataa tggttcggtc cacaggtgac cgtgccgccg ccgtcggcca
    13621 acatggtggt gatcacgtat ccgaggtaga gcccacttga ccgagaaagc tcgaacgcag
    13681 ccccgacgta atccttgacc tcctggggcg tgctgggctc gatgaccggg acgaacagat
    13741 ggcggaagag aaacctggag tcggcaggca cctgggtgct ttcgctccac ggatcatcac
    13801 cgaccacaat gactgctccg ccgtggggat gcgggccggc cagattgccc agggccaagg
    13861 catcgctggc gacgtgcagg cccacgcttt tcatgactgc catggccctc aacggggcca
    13921 tctgcgagcc gttgacggcg gcgaccgagt gagcttcgtt gttcgagatg cggccctcga
    13981 taccgcgttc ccgaaaaagc gacgcgcagg cggcgattac gtcaaagaag gctgaaaccg
    14041 gggaaccggg ataacccgtc aggagatgca cgccgccggc cgtctccaac gcccctttga
    14101 cgaggagttc gttaccgttg aagacatccg tgccttcgtt ccgcaggaac ttggcggcaa
    14161 tagtcttact catgactacg gcgagacgcc tcgggcgctc cttgtatcca agcaggccaa
    14221 cccgcgccag agtcggttct gcctgacgac gcggtatcgg ccgcgtctca tgcttcgtcc
    14281 ggtgcccggc cggtttcccg gcggtctcgc tcggccgacc cgcgaaatcg tacaaacaga
    14341 ctggagagca ttataacgac gcgggcaaga aaacaccacc cacccgttat gctttggaga
    14401 gcggcccgac agactccaga cgagccgccg cgaagtgggg tgcgatcggt cagccccagg
    14461 gcctacatca gctcaacgaa cgactgcccg aggcttgcgg gggcaacccg tgcgaagccg
    14521 caggcggagc aggcgaggcg cattcccgcc ggagcacccc aagcctgatt ccgattggag
    14581 ttctcgtccg accctgatta cactacgccg cgactcaaag acgacgcgag gccgagaatt
    14641 acccggcacc ccgaatgaat caggcggaca acccaattca gcgtcccgca ctccactacg
    14701 cgtggatcgt cctggccgtc gggacacttg tggtcgtcgg ctcactgggg ctggctcggt
    14761 tcggctacac gatgctgctg ccttcgatgc agacggcctt gacgatggat aacaccaagg
    14821 ccggcggcct ggcgacggcc aaccttgccg cctacttggc gctggcggtc acggggggcg
    14881 tcttggcaag ccgctacggt ccaaggatcg tcatcaccat cgggctgtcg gtggccgctc
    14941 tcggcatggc gctgaccggt tcagcagcca gttttggcga ggccatgatc tggagggtgc
    15001 tcaccggctt gggcagcggc gccagcaacg tgccggtgat ggcgctcctt tcggcgtggt
    15061 ttgcgcagcg acgccgcggc ctcgccgccg gcataggcgt gacaggttcc tcgctggcac
    15121 tcatcgtact cggtcccctg gtacctcggg tattggccgc cgacgaggaa aacggctgga
    15181 gaatctgctg gtttctattc gcagcagtga ccttcggttt ggccgttctt tcatgggccg
    15241 ttttgcgtaa tcgccctgcc gacatcggcc tgcacccgtt gggagctgct gaagacgacg
    15301 agcccggcgt cgctgctcag ggcggactcc gttggggccg ggtctacggg tcaggagtcg
    15361 tgtggcatgt cgggttgatc tacattgcct ttggtttctc gtacatgatc tacatgacct
    15421 ttttcaccaa gtatctgatc gcggagggcg gctataccaa agaagctgcc ggtgcgctgt
    15481 tcatgctcgt gggctgggta agcttaccct gtggtctgat tcttgggtgg atctccgaca
    15541 tcatcgggcg caagggtgcg ctggttattg tgtatctgat ccatgccata gccttcgcgc
    15601 tgttcgccct gtggccgtcg ccggccgggt tcacgatatc tgcgatcctg ttcggccttt
    15661 caccgtggac gatcccggcc atcatggccg ccatctgtgg agatgtcctg ggaccgaggt
    15721 tggcacctgc ggggctgggg tttatcaccc tcttcttcgg catcggccag gccgcggggc
    15781 ccattgcggc aggtgctctc gcggatgcag acgccaacaa gtctctttcc tcggcgttcc
    15841 tgctggccgc gggtgtcgcc ttacttgggg ccatcggctc gctgctgctt cgccgcccaa
    15901 agggctaagg cggaccgtct tttcgcaggc cgcaagccaa gcgcaccacg atccgaaaac
    15961 atccgcgcaa gccgcacaga cactcggccg tgtggttcca gttcaaccct cgctgccgcc
    16021 ttgtcgggtc aaacgacgtt cctgggcggg agccgcacaa cctcgacgct ttgcgtgcga
    16081 gaccgcgcgc tggtagtctg ttggctcatt cggtgggcca tggcggtgcc ctattcggtc
    16141 ggcaatgcat gaagcagagt gccagaagtc gcggagttat gacaaaggct ttcggccctg
    16201 ccttgggggt cgagcaacca cacatgatcg tcaaaggaga acggtaatga gtcagaaaac
    16261 caatcgtcgt gatttcctca agagcagcgc cttggcgggc gccggtttct gggttgccac
    16321 ccggagcgcc agagcccaaa gcaagtcgcc caatgagaag ctgaacatcg cctgcatcgg
    16381 aacggggggc atgggcagcg gcgataccga cagtgttgcc agccagaaca tcgtggccct
    16441 gtgcgacgtt cgccccagcg ccctggagag caagctggac aagtttcccg acgccaaggg
    16501 gtacgccgac taccgaaaga tgttcgatga gatgggcgac aagatcgacg cggtcaccat
    16561 ttccacaccc gatcacactc acgcgcacgc cgcgatgatg gcgatgaaac tcggcattca
    16621 ctgcttcgtg cagaagccgt tgtgccacga cgtgtgggag gcccgcgaac tgactcggat
    16681 ggccaaggaa ctgaaagcca agggcggcgg caagctgtgt actcagatgg gtaatcaggg
    16741 gacgtcatcg gccgggcttc gcaaggctgt cgaggttgtc tggtcgggca tgctgggagc
    16801 ggtgcgcgag gtgcacgtgt ggaccaaccg gcccgtgtgg ccacagggta ccggcgccgt
    16861 ccttgatctg gtggacgtcc gctatgcgat gcatggggat gcagggcttc gagtggtgaa
    16921 gaaggagaca ggagagaaag agaaaccgca ccctgagccg cacgacgtgg attgggactt
    16981 gtggatcggg acagctccgt atcggccata tcaaccgcag taccagccct tcaactggcg
    17041 cggctggtgg gactggggca cgggcgcact tggcgacatg gcctgccata cggcgaacat
    17101 ggccttcatg gcctgcaaac ttggttatcc cacccgggtt gaggcggaaa tgtccgaata
    17161 caatccggaa acgttcccca tgtggagcgt gatccgctac gagttcccgg ctcgcgggaa
    17221 catgccccct ctcaagtgga cctggtacga tggggggaac gacaagcccg cctgggtcaa
    17281 caagaagctg aaggaactgg cccacggccg cgacattccc ggtagcggat cgattttgat
    17341 cggcgagaag gccacgctgt tctcgccgaa cgactacggc gaggactggg tgcttctgcc
    17401 cgaaggtcaa aaacgcgacg aggtcaaggt agaccagatt ctgccccgct cccccggcca
    17461 caaggaggag tggttcaacg ccatccgcga aaacaagccc gaactggcga tgtcgaactt
    17521 ctcctatgcc ggcccgctga ccgaaacggt ggtgctgggc tgtgtggcga tgcgggtggc
    17581 gcctggcatg gaagtcgagg tggaacaacc ggacggcaag aagaagaagg tgagtcacaa
    17641 gcacattgag tgggatggac cgaacatgaa gatcaccaac attccggccg caaatgcctt
    17701 cctcaagcgc gagtatcgca aagggtggga attgtagaga gtacggggca ggcacaatcc
    17761 gaaacgctga tcatcccagg ggcgggcagc cataaacggc tgcccgcctc gtttcgtgtt
    17821 gggcgctgca gacaagccgg aggtgtcgat aggcgaacaa cagtaaccac ccgtgccgca
    17881 cgaactcatt gggcctctgg acggccggtt gcggttctgc tagaatggta gacggatgtg
    17941 gggatcgctc ggtcctgcgg ttttgaccgc atttcaacct atccggaggt atatgcagtg
    18001 gcaaagaagc tctgctctgc gtggatatca ggcgtcaaca tgatggtgta cctggcggcc
    18061 ggcgccgctg cgtcggatac gcagacgatc aacgtctgtt ttgtgagaca aggcgctctc
    18121 ggggcggtgc atcgcacggc aagtccgagt acgccggcca ttcaaacagc catcctcgag
    18181 ctggtggcgg gcccaaccgc gcaggaaggc gccgcaggat tcacttcatg gattccctcg
    18241 ggcacgaagg tcgtcaacat tcgtctttcg gacgacgggg tcatcgtgga cctctcacct
    18301 gaagttttgc aggacctcga tgagtccacg ctcttgggta tcttcgacca gttccgcgca
    18361 acgctatggg cattcccgga cgttttgagc atccacctga cctgccaggg ccagttgctc
    18421 gcctcctatc tcccgcccgc ccccgaaatc gaatcgatgc ccgagacgat gagccaaccg
    18481 atgcgattga gcatgatgga gcccgaacca gagcagagct tgctgaacgg cacggcgctc
    18541 agcggccgga acatcaccat cggaccgtcg cacggacgct actggcacga cgactacggc
    18601 tggatatggc aacgaggtga tccctgcggc tttggggagg ccgtgctcga ggacaccaac
    18661 agcattcgcc tgtgccagtt tctctatcag tacctggtcc aggacggcgc gacggtccat
    18721 gtcccgcgcg agatgaacga aagcaactgc tgtcactcgg ccacgggtct gccgtggtgg
    18781 aagatggccg cacgatattg gctgcaggcc aacggcttac cctcatcgat ctgggacagc
    18841 tcgaccacag attacaatga cgacattcgc gccaggccgc tcttcgccga ttaccgcggg
    18901 tccgacattt acatcgctca tcacaccaac gcttttgatg gcacggcctc cggcaccgtc
    18961 acgtatcgcg acacggccat ggagcatccg gagcacgagg ccaacagcta cattctggcc
    19021 cagagagtcc agagcaacgt ctgcgacgcg atcaagaaca tctatggtat cgccgactgg
    19081 tacaaccgcg gcgtgctcga cgcaaagggc ggcttcggcg agatccgcat ccccaaccgc
    19141 ccggcatgcc tgatcgagct cgcctttcac gacaactgta ccagagacgc cctgtacctg
    19201 acggacaact tcttccgttc ggtagccgag tggggactgt acaagggagt ctgccagtat
    19261 ttcggcgcaa gcccgacgtg ggactgttac tccgacgagt acgtgagcga caccatcccg
    19321 gccatcatga acccgagtca gtcgtacggc gtcagtatca ccttccgcaa ccgcggcgtg
    19381 gtctggtcgg aggcgaggag ttttcgcctc ggcgccgtgg gggacagcga cccattcacc
    19441 gcctttaacc gcgtgaacat ctccggggaa gtcggtcccg gccagacgta cacgttcagt
    19501 ttcaccatga ccgccccggc cgctccgggc acgtacatca ccgactggcg aatggtgcga
    19561 gacggcgtga cctggtttgg tgctacctgc agcaagcagg tcgaggtggg cacgtccggg
    19621 ccccagcctc ccagtattgt ccaacacccg gcagatcaga ccgtgcctct gggtactgtc
    19681 gccgccttta ctgtgcaggc cacaggtgac agtcctctct actaccagtg gcaaaaggat
    19741 accgtggacc tgagtgacgg cggcaagatc tccggggcca actcggccac gcttcagcta
    19801 accggcgtcg acgttcccga tcagggcgtt tatcgctgcg ttgtgacaaa tgccctgggc
    19861 agcgcgacct caaacgcagc cactttgacc gtcactgctc cgatcttcat ggttgagagc
    19921 cgctccggag gccagaacta cgccaagtac tcggagaccg gagggtgggc caacagcggc
    19981 ggcaagagca ccgcagcagg cgtcacggcg ggcataggat cgcgctacgg cagcacctac
    20041 cgttcggttg ccggcgagaa gcatgccatc tttggcgcag acctgcctgc cgccggcacg
    20101 tacgaagtct cggtcacatg gggcgcgaac gcgaatcgtc gcactcccat cctccaccgg
    20161 atcgtgcacg cgggcgggac caccgacgtc aacgtcgatc aagccgccac cgcgaatacg
    20221 tgggtatcgc tcggcgtcta cacatttggc gccggcagcg acgttggaag cgtggacgtc
    20281 aacaacctca atattgatgc cagcgggagc atgtacgccg acgcggtgaa atgggtttac
    20341 gtggcaagcc cgccggtcat cgatcagcat ccggcggttc aaagcgtctg tccgggcgcc
    20401 acggccacct ttaccgtggc ggcctctggt ggcggcacac tcgcctaccg gtggcagaag
    20461 aacggcgtca acctgaccaa cggcggacat tactcggacg tgacgaccgc aactctgaag
    20521 gtattgagcg cggacgtcgc cgacgtggcg aactaccgat gcgtggtaac aaacatttcg
    20581 ggcagcgcga catcaaatga ggccggactg acgctgaagg ccgcgacgag catcactcag
    20641 catccggagt cgcaaaccgt ttgccccggc tcggcaacgg gcgttcagtt cgccgtggtc
    20701 gcaaccggtg acggcacggt ttcctacgaa tggcagaaga acagcgtcaa gctcaccgag
    20761 ggtggacact actcgggtgt cacaagcccc gcgctcacgg tactgaacgt cgacagcagc
    20821 gatattggga cctaccgctg ccttgtgacc gcaggctgcg gcaccgcgac gtcgaacgag
    20881 gccgttttgg cgctcaggcc gatggtaacc gccgacttcg atgaggactg cgacgtcgat
    20941 caggccgact acgacctgtt cgccgcatgc agctcaggac ccggtgtgcc aaccgggacc
    21001 ggctgcaatg ggaaggatct ggacggggac ggagacgtcg atcaggccga cttcggcgtt
    21061 ttccagcggt gctacagcgg cgagaacctc ccgccggacg ccgactgtgc caactgacag
    21121 taacaggcga gcggtcaaac acgggccagc ccgcatgttc cgcccgtgcg tcagcccgga
    21181 acctctggtc cctcggacat acgaggcacc gtcaaactca gattgagagg cgcgacgcac
    21241 gcgccgaggg
//
LOCUS       DAOTPW010000084        21001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2580_length_21001_cov_5.732121, whole genome shotgun sequence.
ACCESSION   DAOTPW010000084 DAOTPW010000000
VERSION     DAOTPW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2580_length_21001_cov_5.732121"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..916
                     /locus_tag="P5316_09405"
     CDS             65..916
                     /locus_tag="P5316_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85215.1"
                     /translation="MSLTELDNLLRRFHRRINQIAVIRILAIVTVALLAVWSTWEPAQ
                     LDRRVAALLVSVVLLGWIALALVSFHLARMLQMARILLGTGQTDNARVWLGRVMTGWS
                     LSSRIQLVACQHLASSFLAKDRHEEVVSICGALLRHRLGRLKNVAVNARLMLADSLLM
                     LDRVGEACEALEPLYDLPLSLADRMKLLPIQLRYELRAGQPALAAESLSENVRIAELL
                     ESPQAALVHAMLAKACRRKGMHAQAEFLIRRARLYADLEPLAQRYKEIADVTEADGGP
                     DAHAPFP"
     gene            1932..3125
                     /locus_tag="P5316_09410"
     CDS             1932..3125
                     /locus_tag="P5316_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRR85216.1"
                     /translation="MSVMTSHEFLDKVIRATPDGEHIRRCLQCGTCSGVCPYGFAMDH
                     PPRSLIAALRADDWQGVLESDTIWLCVSCFACASVCPVQIPLTRGLLSALKTEMLVGG
                     SIPPELQTALGNSRRYGNPMGESPRKRADWAKDFTIPVPVLANHKDPVDVLWFVGDYG
                     SYHPRVQSVSKAMAKVLHALHVTFGILGPEENSDGDTQAFTGEWGLFELLARKNIKAM
                     TKYSFREIITGDPHAFNALKHEYPRLGFDRPVRHYTQFLFERIDQLRRLFKNPLSMRV
                     TYHDPCYLGRANENNIYEEPRRLIEAIPGVELVEMSHNRALSLCCGGGGGGMWLDGFS
                     WEKAHARTTEWRIKEAIAVGADVLAVACSYETPRFEDAVKSTGNEGKLMVRDIAELLA
                     EALFG"
     gene            3137..3907
                     /locus_tag="P5316_09415"
     CDS             3137..3907
                     /locus_tag="P5316_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRR85217.1"
                     /translation="MDIIVLVKFVPDLVEDLEIDAQSGLLDRSFLRLIPNELDEHALE
                     EALLLKERHGGTVTVITVDTGDVDESLYTAAAKGVDRLIKITGEGFDQGVTNQALAVI
                     FQSVMGDMAFDLILTGTQAVDDLDGFVGAMLAERMGLPYVGYVTRVEAENGTMVARKE
                     YPGGLNADIEVRQPAVLGIQAAEKPPRYVVTSKVMEAMRSATIDEIEAPETPIGGSLP
                     VSRMSVPETGAHAEMIEGTLEQIAEAVANRLREQGILA"
     gene            3920..4900
                     /locus_tag="P5316_09420"
     CDS             3920..4900
                     /locus_tag="P5316_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRR85218.1"
                     /translation="MTGEVCVVAETLRGEVTELTYTMLAAGRQLANGLKTRLSAFLIG
                     HQVERMTASLGAADRVVCVDDPALVEFNPQAYLHVVAEIFGRHTPRVGLFGHTATGTD
                     IACGVAQRLGAPIAVSCRTFTTEGGEPHYSSLICGGKIIAEGPLPGPCCIATVMTGGY
                     KAEQGKVVKAAPVESLPAPEGLDALRTRFKRYIEPPAGDVDITKQAIIVSVGRGIQNK
                     DNLAMAEELAKALGGVVSGSRPVIDQGWLPTTRLVGKSGKQVKPKVYLALGISGAPEH
                     AEGMRDAEMIVAINRDEKAPIYGFAHVGAAVDVLEFVPVLTDKIKHIHCA"
     gene            4910..6964
                     /locus_tag="P5316_09425"
     CDS             4910..6964
                     /locus_tag="P5316_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRR85219.1"
                     /translation="MIERINFWGIPHGVHLLGYVVPISCAVVLIWRLGARARLWWKVG
                     RPERRWDHIGLRLRRVAKYAIAQVKVLRQRFPGFAHVAIAWGFFVFFLGTALATIDAD
                     LVRFLRGPVYLGFKVLLDVFTALALVGILLAAYRRFLQRPERLTLTRAFSFSLGLVFV
                     VVLSGLLTESLRLAAVAKDPMLQPGWTPSLAWWTPAGWVTAQIWLASGLSSAAIDQLH
                     LAMWLGHVALVGLLFVVIPIGPLLHVLTAPLNVFFSPIDRPIGRAAPAAATDGGGAGT
                     LRAFTWAQLMQGDACTECGRCQDACPAYGAGQPLSPKKLVLAIKDSLAPTAKSMAGNG
                     MPAFVGGVVEDPTAWACTTCRACATECPVLIEHVDLILDMRRFLLAQQRADNQLTGAL
                     SNLRRYGNSFGKSDKQRAKWTTGLDFKIKDIRKQPARTLWFVGDYASYNPALTEATMA
                     AARVFQRAGVDFALLYDAERNAGNDVRRSGEEGLFELLVQKNSVAMNKCEFQEIVTTD
                     PHTYNTLKNEYPWNGRAVQILHYSELLDRAIREGTLPIRRKLGCTVTYHDPCYLGRYN
                     NVYDAPRRVLEAIGCRVVEMPRNRDRALCCGAGGGRIWMDEGQIKERPSESRVREAAG
                     LDGVQIFVVACPKDLTMYRDAVKTAGLEGTIVVKDLIELVEEATREENAVAPIEAATT
                     SA"
     gene            7017..9074
                     /locus_tag="P5316_09430"
     CDS             7017..9074
                     /locus_tag="P5316_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HRR85220.1"
                     /translation="MAEQARRDGTERVGVYVCHCGSNIAATVDVEEVARWAGERLPGV
                     VVSRDYKFLCSSLGQAMIQEDIKEHGLTRVVVAACSPHLHEKTFRRACANAGLNAFLL
                     QMANIREHVSWVTKHKETATEKVKTLVAGAVARVQHHQPLEPMPVPVNKHTLVVGGGI
                     AGIQAALELGDAGYPVYLLEREPSIGGHMAQYDKTFPTLDCAACILTPKMSEVGQHDH
                     IKLLTYSELEEVSGSIGDFHVRIRRKARHVKEADCNGCGLCTEKCPRKVVDEDFEAGM
                     GYRKAIYAPFPQAVPKVPVIDTKSCIWFERGKCRVCEKVCPTHAIDFDQEDEILEVRV
                     GNIILATGWDLFDCRRIPQYGYGRLANVFTSLEFERMCNASGPTGGKIVLRDGVTEPQ
                     SVCIVHCVGSRDHRYNTYCSGICCMLSLKLGHLVMEKTGAKVYACYIDMRPNKKDYEE
                     FYWRLLDEGMLFVRGKVAEVTDIARFPNEEGKLIVQVEDTLLRKQRRIPVDMVVLMGA
                     IEPRRDARETGLKVGISCSTAGWYIERHPKLDPIATMTEGIFIAGACQGPKDIPDAVG
                     QGAAAAARVQGMISKGTVMIEPVVAGIDEDHCSGCRICNNLCPFNAIDFIEDEKVSRI
                     NAVLCKGCGTCVAACPAGAISGAHFSNAQILAELEGLLCDAGGENELIAGASGKPSEP
                     VAV"
     gene            9085..9582
                     /locus_tag="P5316_09435"
     CDS             9085..9582
                     /locus_tag="P5316_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRR85221.1"
                     /translation="MEQETFEPKIIGFFCNWCSYRAADGAGTARIKHSPNVRIIRLMC
                     SGRVDIAFILKALSLGADGVLVAGCHPGECHYIEQNYKTIRRFTMLRHTLRAFGIDER
                     RVRLVWASAGEASHLAEVIDEMVEDVRTLGPLNWRRNWAEDGAHLDDVERILHEHEEA
                     MEVSR"
     gene            9579..10598
                     /locus_tag="P5316_09440"
     CDS             9579..10598
                     /locus_tag="P5316_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRR85222.1"
                     /translation="MSEKGKLAIYWAASCGGCEISILALNEKILDVANAFDIVFCPCI
                     MDTKARDVEKMADGTIDVCLFNGGIRTSEQEYMAGLLRRKSKVLVAFGSCAYEGCIPG
                     LANLTNRKEIFETCYIDCPSVDNPNEAVPVADTRVNGYRLTLPVFYDTVRTLGQTVKV
                     DYYLPGCPPEAPRIWDAVAAILEGKLPPPGSVIGPETTVCDECQRKREEKKIKRFKRT
                     WEVIPDEDKCLLEQGLLCCGPATRAGCGALCPQVNSPCIGCYGPNAGVEDFGARLMSA
                     LASVIDATDPDEIDEIIRQGIPDPVGTFYRFGLPHGFLRRAAAIPDAQRHELPLPKPA
                     EERSK"
     gene            10601..12100
                     /locus_tag="P5316_09445"
     CDS             10601..12100
                     /locus_tag="P5316_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRR85223.1"
                     /translation="MTAKHIVIDPITRLEGHGKIDIFLDETGDVADAFLQIPELRGFE
                     RFCVGRPAEDMPNLTSRICGVCPEAHHMAAAKALDALFHVEPPPTGKKLREMFYSIFC
                     ATDHTTHFYALGGPDFVMGPDAPKEERNILGVIKKVGMEIAGRVIKMRADGHGLIKMI
                     GGRAVHPNWAVPGGVSRGITEEQRREIERLGRDAIEFAKFSLQLFNQVVLGNSEYVKL
                     IQSDAYTHRTYNMGTVDAQNRVNFYDGMIRIVGPDGREHAKYHPREYREYIAEHVEPW
                     SYLKFPFLKKIGWKGFVDGEDSGLYKATPLSRLNAAEGMATPLAQQEYERMYDALGGK
                     PVNHTLATHWARLIELLYATERWVELATDPGITGKEYRVLPSETPDEGVGSVEAPRGT
                     LTHHYWTDERGILTRVNLIVGTTNNYAPIQLSIKTAARSLIRKGTAVSEALLNKIEMA
                     FRAYDPCFGCATHCLPGQMPMTVVIHSADGRTVHRLSRGEGRGIGEPVR"
     gene            12097..12609
                     /locus_tag="P5316_09450"
     CDS             12097..12609
                     /locus_tag="P5316_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRR85224.1"
                     /translation="MTNALAACSQGRASGEGPTLVLGLGNPLLTDDSVGLRVIDHLRP
                     QVAGWPNVQLDEDYCGGLGVMERMIGFDRVILVDAICTGGRPGAVHVLTVRDIPTRHS
                     GSFHDADLRTALALGRQAGARLPADENIRIVAVEAAEVLTFGQQCTPAVRSGISRAAE
                     SVLKLLTAWS"
     gene            12614..13060
                     /locus_tag="P5316_09455"
     CDS             12614..13060
                     /locus_tag="P5316_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRR85225.1"
                     /translation="MVPVETLRSLACLAGVPVDSLKALAAIAEERDFKAGEVLWHEGD
                     PVRWMCVVRQGEIDVIYQLQGGRQCVVDTVVGGEITGWSAFVEPHRHTATCVARASGR
                     ALYLEAAPMRALCEKDSLLGYRLLKQVAKTLSSRLESARLQLVATG"
     gene            13171..13572
                     /locus_tag="P5316_09460"
     CDS             13171..13572
                     /locus_tag="P5316_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR85226.1"
                     /translation="MSRRILLIDDDPDMHLVMKMILEPEGYEVTCCRTGSHGLETMRR
                     QRPDLVLLDIMLADPSEGLRVACEMRHDEKLKGIPIIMISAIGEQIGQDYYSEVCPGT
                     ASGDLFLEKPFDPPTVREAVKWILERPAVES"
     gene            complement(13597..15198)
                     /locus_tag="P5316_09465"
     CDS             complement(13597..15198)
                     /locus_tag="P5316_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR85227.1"
                     /translation="MLIQVRPVVEDDVMRSPEVEKTDWIQVLAHLEGDPRAVALVPML
                     RLHWFIRLRWIFLAGAVAALALERFVAPVTQRPFDLAVVLIVLALVNLVWLIVSRSLF
                     RHVTRDPKALRAQIERLAWFANAQVATDLLLLTAILRYTGGVENPVAIFYLFHMSIVS
                     LLLKRWHAVLQGIWAWALYAVLVIGEWRGWLAPHYDFLPSVSVGLYARPEYVLAALVV
                     VACGIFGTLYFTLHIAGHFQTQERQLQQANAALKQSQRAIQDLQRRRSRFMQTAAHQL
                     KSPLAAIQTMVELVRTGLVPPEAVSGTCEKIIHRCQEGIAQVTELLTLARVQEADPSR
                     HRQSGSDVRETVNELFGRFRPLADSKQIKLVCSMPSDEELTVCVDPRDLRDCIGNLID
                     NAIKYTPGPGSVTVTVMPERSNGELMAVLVNVTDTGMGIDPHLAVSPDGEPGHEAVFD
                     AFRRGGNVVAAGIPGTGLGLSIVREVVEQAGGQIVVSSCPGQGSSFTLTFPSPSGSIR
                     QPQVRNTRASQVVLEESEAPAAPQD"
     gene            complement(15390..16712)
                     /locus_tag="P5316_09470"
     CDS             complement(15390..16712)
                     /locus_tag="P5316_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR85228.1"
                     /translation="MLTLHVPVSGRPPQEPAIGPAPWIVVSPPGPMTALALAHDRRVA
                     ASPSGWPYPLVVRRARGSVVEDLDGNRYLDFTAGGLAFVAGHAHPMVVRAIQEQVERM
                     AKACSGEFTSEPLMGLARKLAQLAPTRGENRVLFSNSRMQSLEVVLRMARRRNTRRRI
                     VTFNGNSHNRGLAAITLEFGRPRHRRRIPPEAWVVDSLPYGNVAAVHDYFRSGLGDEV
                     GVLFAEPMLTEEDYHVPSSDFLPELRRVCDEHDVLLVIDETRTGMGRTGRMFGCEHFA
                     TQADVLLAATGLNSGMPIHALIAPETLLSSDEGLVTAPYVGNPVSSAASLASVEALEK
                     HLIANAARLAPIFISKLEQIDGRRRCLGSPRGMGFLLAVDVRKPRRSQTSAAQLRERI
                     ITESFRRGLLLWGCGDTTIRFCPPLCINQAQLEVGLNVFEEAVATVAS"
     gene            17313..18254
                     /gene="mdh"
                     /locus_tag="P5316_09475"
     CDS             17313..18254
                     /gene="mdh"
                     /locus_tag="P5316_09475"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRR85229.1"
                     /translation="MTGRAKITIIGAGNVGATCAHWAAAKELGDIVLVDIVEGLPQGK
                     ALDLREASPIEGFDCEIMGTNRYEETADSDVIIITSGLARKPGMSRDDLIAKNTEIVG
                     SVAKQAARYSPQAVVIVVSNPLDAMVYVAWKASGFPTHRVVGQAGILDTARFRTFIAM
                     ELGCSVEDISTLLLGGHGDDMVPLPSCTAVGGIPVTSLIPKDRLDAIVDRTRKGGGEI
                     VGLLKTGSAYYAPAAASVQMAEAIIRDKKRVLPCAAYCDKEYGIGGYFVGVPCILGKG
                     GVEKVLEVPMTPVEKAAFQVSIEHVKELVAVVQRIGA"
     gene            18326..19081
                     /locus_tag="P5316_09480"
     CDS             18326..19081
                     /locus_tag="P5316_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRR85230.1"
                     /translation="MRLFVSMIAGGCVIAAVGCTPEQVKPGIAGKQQPVAEKVEIKRV
                     VDCRYLLYLPENYESQDRWPLILFLHGAGERGDDIEKVKIHGPPKLIAQGKHFPFIVV
                     SPQCPNGQWWDIEVLGALLDRIERDYKVDRDRIYVTGLSMGGFGTWALACAQPDRFAA
                     IAPICGGGEPKLAGQIAHVPAWVFHGDADNVVSVSRSKEMVEALKTAGGDVKLTVYPE
                     VGHDSWTRTYDDPALYDWFLSHRRGKTRTRESK"
     gene            complement(19164..19703)
                     /locus_tag="P5316_09485"
     CDS             complement(19164..19703)
                     /locus_tag="P5316_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85231.1"
                     /translation="MNPRIRHAVMPALMVIFLVSGAGCPKESPNKKSPEPKGLALPVE
                     QPLAQTRPVATKPAESRPVASQPVSTYDSNPPYRVKLYVSNPSDKQPGWLRILKLNDE
                     NQTATAEGFFPEQNDVNVTTGNVQQIEVHINFLPLAPRKRTFLRVDGQVIEIVTRDRP
                     YVVLERSPAGVWKVLPQKK"
     gene            19954..20502
                     /gene="hslV"
                     /locus_tag="P5316_09490"
     CDS             19954..20502
                     /gene="hslV"
                     /locus_tag="P5316_09490"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRR85232.1"
                     /translation="MRQGETAIQATTILAVRRGEQTAIGGDGQVTIGQTALKHDANKI
                     RRLSGGKVICGFAGSAADAFALLDRFEKKLDEFKGNVRRAAIELAKDWRTDRVLRHLE
                     SMLAVVDSQTSLIIGGSGDVIEPTEGVIGIGSGGMYAVAAARALLKHSNLPAADIVRE
                     ALAIAADICVYTNHNIIVETLP"
     gene            complement(20601..>21001)
                     /locus_tag="P5316_09495"
     CDS             complement(20601..>21001)
                     /locus_tag="P5316_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85233.1"
                     /translation="IKHLRKTVAGSPSSDPPRTPRPTSPPPHILGRKDADLPTINGPI
                     ATPTAEPPIESQESVQWFTTTAGIGGVTREPLPAGQTVLATLSLTISPEPGTYHLTLT
                     DAQFVDTIHVTHPATTGQPLEIVVSEQSVD"
BASE COUNT         4064 a         6024 c         6926 g         3987 t
ORIGIN      
        1 tggtggaccg gtttggggag atggctcccg tggccgcccg gtacgcggct gtggagcatg
       61 tgctgtgagt ttgacggagc tcgacaatct gctgcggcga ttccatcgtc ggatcaacca
      121 gatagcggtc atccgcatat tggcgatagt gaccgttgcg ttgctcgcgg tatggtcaac
      181 gtgggagccg gctcagttgg accggcgggt ggccgcgttg ttggtgagcg ttgtgctgct
      241 ggggtggatc gctctagcac tagtcagctt tcacctggct cgaatgctgc agatggcccg
      301 catcctgctg ggcacgggcc agacggacaa cgctcgggtg tggctgggcc gggtgatgac
      361 cgggtggtca ttgtcgtcac gaattcaact ggttgcctgt cagcatctgg cttcatcgtt
      421 tttggccaag gaccgtcacg aagaggtagt gtcgatctgt ggcgcgctgc tgcggcatcg
      481 tttgggccgc ctcaagaacg tagcggtcaa cgctcgcctg atgctcgccg acagcctgtt
      541 gatgctcgat cgggtgggcg aggcttgcga ggccctcgag ccgctgtacg acttgccgct
      601 gagcctggcc gaccggatga agctgctgcc aatccagctt cgttacgagt tgcgggccgg
      661 gcagccggcg ttggcggccg agtcgctgtc ggaaaacgtg cgaatcgctg aactgctcga
      721 gtcgccgcaa gcggcgctgg tacatgccat gctggccaag gcctgtcggc gcaaagggat
      781 gcatgctcag gccgaattcc tgatccgccg agcccggctc tacgccgacc ttgaaccgct
      841 ggcgcagcgg tacaaggaaa tcgccgatgt caccgaggcg gacggcggac cggacgcgca
      901 tgccccattt ccgtaaggcc cagacgcata acagtttggg gcttcaagta cccgcctttc
      961 cgcgattccg aaatgctgct cgtttgggct ctgagtttcc ggaccagaat ggctaaaacg
     1021 tgccatggcc tgggccttga ttctgtgaaa gttttcacgt atccttgtga aggatttcac
     1081 taatcaagct gcgggatact caggaggcct gtcagccgta tcccgcggtg gtttgggcgt
     1141 cagttcgagc aaatggtctg taatacgcgt cagtgtgagc gagtgtgttt ggtttctccg
     1201 gcaagttcgg cactcgtgat cgcattagtc tgtttttttg aattgccgat gcgcggtggc
     1261 gaacggctga aatagggcca tagcgaggat tcgggctctc gagggggccg gcattcggtt
     1321 gaggctatgg gaggccaccc catggcaacg tgggaagtgc gcgcgctttt cagaggtttc
     1381 taccgccaac ctgcggcgga gcgggggtac agcccctggt cccgcgtccg aatctcaaga
     1441 agcaccacag aagcggttga aaaagtgagc atagtcgcgc aagaggcgtt tgtgcgacct
     1501 tggcgcgcgg cttgggcctc aagccggcat tgtccgtcgc aggtttttcc gtccatggcg
     1561 gcgagcgagt ccgttggagc agtcgcgcgc cggccgcgaa aactccggag cgtacggcga
     1621 cttgcccgtc aggaacctcg ttcttgtttg cagattcatc aaccaagatc cgggcacgag
     1681 actgacaccg gtcgggcgga gcgtggccac cgatgccttg tgtcgggcgg tcttgttgcc
     1741 acataccgta aacggcagcg gggtgagccc gaaacgcgtc aatcgggtgg ccagtcagtt
     1801 ccggtcggcg cgggaccccg ctgtcgttgt cagggcggtg ccggcaggca atcgccgacg
     1861 gcagggcaac acaagtattg tcctcatggt aatcgccgga tgcggtttct ccaagcgtga
     1921 ggtatctcaa catgtcggtc atgacgtccc acgagtttct cgacaaggtg atccgggcaa
     1981 ctcccgacgg cgaacacatc cgcagatgtc ttcagtgcgg aacatgcagc ggtgtgtgcc
     2041 cttacggctt tgcgatggac catccgcccc gatcgctgat tgccgcactt cgcgccgacg
     2101 actggcaagg tgtgctcgag agcgacacga tctggctgtg cgtctcgtgt tttgcctgtg
     2161 cgagcgtctg tccggtccag atacctttga cgagaggcct gctttcggcg ctcaagacgg
     2221 aaatgcttgt cggcgggagc attccgccgg aattacagac cgccttgggc aattcgcggc
     2281 gatacggcaa tcccatgggc gaatcgccgc ggaagcgcgc ggactgggct aaggatttca
     2341 ccatcccggt cccggtgctg gcgaatcaca aggatccggt tgatgtgctc tggtttgtcg
     2401 gtgattacgg ctcgtatcac ccgcgtgttc agtcggtcag caaagcaatg gcaaaagtac
     2461 ttcacgccct gcacgtgaca ttcggcatcc tcggcccgga ggagaacagc gacggcgaca
     2521 cgcaggcgtt cacgggggaa tggggactgt tcgagctgct cgcccgcaag aacatcaagg
     2581 cgatgacgaa gtattcattc agagagatca tcaccggcga cccgcacgcg ttcaacgccc
     2641 tcaagcatga gtatcctcgt ctcggttttg acaggccggt aaggcattac acccaattcc
     2701 tgtttgagcg catcgatcaa ctcagaaggt tgttcaagaa cccactttca atgcgggtga
     2761 cgtatcacga cccgtgttac ctcgggcggg ccaacgagaa caacatttac gaggagcctc
     2821 gccggcttat cgaggccatt ccgggcgtcg agcttgtcga gatgtcgcat aaccgtgccc
     2881 tgagcctgtg ctgcggcgga ggcggaggcg gaatgtggct ggacggtttc tcatgggaga
     2941 aggcccatgc tcgaaccacg gagtggcgga tcaaggaagc gatcgcggtt ggagccgacg
     3001 tcctcgccgt ggcctgttcg tacgagacgc ctcgcttcga ggatgcggtc aagagtaccg
     3061 gaaatgaagg caaactcatg gtgagggata tcgccgaact gctcgctgag gccctattcg
     3121 gctaggagat cacgctatgg acattatcgt tctcgtcaaa tttgttcccg acctggtgga
     3181 agatctggag atcgacgcgc agtccggtct gctggatcgg agcttcttgc ggctgatccc
     3241 caacgagttg gatgagcatg ccctcgaaga ggcgttgctg ctgaaggagc ggcacggcgg
     3301 aacggttacc gtgatcaccg tcgataccgg cgacgtggac gaaagccttt ataccgctgc
     3361 cgccaagggc gtcgaccggc tgatcaagat caccggtgag ggcttcgatc agggcgtcac
     3421 gaaccaggca cttgccgtca tttttcagag cgtgatggga gacatggcgt ttgatctcat
     3481 cctgaccggc acgcaggcgg ttgacgatct cgacggcttc gtcggcgcga tgctggccga
     3541 gcgcatgggc cttccctacg tcggctacgt cacccgggtg gaggcggaga atgggacgat
     3601 ggtcgcacgc aaagagtatc caggcggcct gaatgcagac atcgaggtcc gtcaaccggc
     3661 cgtccttggt attcaagcag ccgagaagcc gccccgttac gtggtcacca gcaaggtgat
     3721 ggaggcgatg agatcggcga cgattgacga aatcgaggcc cccgagacgc ccatcggcgg
     3781 ctcacttccg gtgtcgcgca tgtccgtgcc cgagacgggg gctcatgcgg aaatgatcga
     3841 aggaactttg gaacagattg cggaggccgt ggccaaccgg ctgcgcgaac aaggcattct
     3901 tgcctgagga gacatcagca tgaccggcga agtctgcgtt gttgcggaga cgcttcgagg
     3961 cgaagtgacc gaactgactt acaccatgct ggcggccggg cgccaactgg caaacggatt
     4021 gaaaacgagg ctgtcggcgt ttctgatcgg ccaccaggtc gagcgcatga ccgcatcact
     4081 gggggcggcc gaccgggtgg tgtgtgtgga tgatccggcg ctggtcgagt tcaatccgca
     4141 agcctatttg cacgtggtgg cggagatctt tggccgacac acgccacggg tggggctgtt
     4201 cgggcacacc gcgacgggaa ccgacattgc ctgcggggtg gcgcagcggc tgggggcgcc
     4261 gattgcggtg tcgtgtcgga catttaccac agagggcggc gagccgcatt actccagtct
     4321 gatctgcggg gggaagatca ttgccgaagg accgcttccg ggtccttgct gcatcgcaac
     4381 cgtcatgacg ggcggttaca aagcggagca aggcaaggtt gtcaaggccg cgccggtgga
     4441 gagtctgccc gcgcctgaag gtcttgacgc cctgcgtacc cgcttcaagc gatacatcga
     4501 gcctcccgcc ggcgacgtgg atatcaccaa gcaggcgatc atcgtttcgg tcggtcgggg
     4561 aatccagaac aaggacaacc tggcgatggc ggaagaactg gccaaggcgc tgggaggcgt
     4621 cgtgtcaggg tcccggccgg tcatcgatca aggttggctg ccgacaactc gcctggtagg
     4681 caaatcgggc aagcaggtta agcccaaggt gtatctcgcc ctgggcatca gcggggcgcc
     4741 cgaacacgcc gaaggcatgc gcgatgccga gatgatcgtc gcgatcaatc gcgacgagaa
     4801 ggccccgatt tacggatttg cgcatgtggg tgcggccgtg gacgtgctcg aattcgttcc
     4861 ggttctgacg gacaagatca aacacattca ctgtgcgtag ggcagtgcga tgatcgagcg
     4921 tatcaacttc tggggcatac cgcacggcgt gcatttgctc ggctacgtcg ttccgatttc
     4981 ctgcgcggtg gtactgatat ggcgtcttgg ggcccgagcc cggttgtggt ggaaggtcgg
     5041 ccgtcccgaa cgccgatggg atcacatcgg tctgcgtcta cggcgggtgg ccaaatacgc
     5101 tatcgcccag gtcaaggtac tccgccagcg gtttcccggc tttgctcacg tcgccatcgc
     5161 gtgggggttc tttgtcttct ttctgggtac ggcactggcc acgattgacg cggatctcgt
     5221 gcggttcctc cgcgggcccg tgtacctggg attcaaggtc ctgcttgacg tctttacggc
     5281 cctggcgctc gtcggcattc tgttggcggc atatcggcga tttttgcaac gacctgagcg
     5341 gctgacgctc acgagagctt tcagcttctc gctggggctg gtcttcgtgg ttgttctgag
     5401 cggactgctg acggagtcgc tgcggctcgc cgcggtagcc aaggatccga tgctgcaacc
     5461 cggatggacg ccctcgcttg cctggtggac gcctgcgggc tgggtcaccg cccaaatctg
     5521 gctggcatcc gggttgtctt ccgccgcgat cgatcaactg cacttggcga tgtggcttgg
     5581 gcatgtggct ctggtcggat tgttgtttgt cgtgattccg atcggtcctc tgctgcacgt
     5641 gctgacggcc ccgctgaatg tctttttctc tcccatcgat cggccgatcg gccgggcggc
     5701 gcccgctgcc gcgactgacg gcgggggcgc gggaacgctg agggcgttca cctgggcaca
     5761 gcttatgcaa ggcgatgcct gcacggaatg cgggcggtgc caggacgctt gtccggcgta
     5821 tggggccggt cagccgttgt ctcccaagaa actggtgttg gcgatcaagg attcgcttgc
     5881 ccccacggcg aaatcgatgg ccggcaacgg catgcccgca tttgtcggcg gtgtcgtgga
     5941 agatccgacg gcctgggctt gcaccacgtg ccgggcttgt gcgaccgagt gtcccgtgct
     6001 gatcgagcac gtcgatttga ttctggatat gcgacggttc ctgctggccc aacagcgggc
     6061 cgacaaccag ttgaccggcg cactgagcaa tctccggcga tacggcaact cgttcggcaa
     6121 gtcggacaag cagcgggcaa agtggacgac cggcctggat ttcaagatca aggacatccg
     6181 caaacagccc gcgcgtacgc tgtggtttgt cggcgattat gcgtcataca accccgccct
     6241 gacggaagcc acgatggctg ccgctcgcgt tttccagcgg gcaggcgtgg atttcgctct
     6301 tctgtacgat gccgagcgga acgcgggcaa tgacgtccgc cggagcggcg aggaggggct
     6361 gtttgagctg ctcgttcaga aaaactcggt cgccatgaac aagtgcgagt tccaggagat
     6421 cgtcacgacc gatccgcaca cctacaacac gctgaaaaac gaatacccgt ggaatggtcg
     6481 cgccgttcag attctgcact attcagaact gcttgatcgg gccattcgcg aagggaccct
     6541 gccgatccga cgcaagctgg gctgcacggt cacctatcac gatccgtgct acttaggacg
     6601 ttacaacaac gtttatgacg cgccccggcg cgtgctcgag gccatcgggt gccgggtggt
     6661 cgagatgccg cggaaccgcg accgggcgct gtgttgcgga gccggcggcg ggcggatctg
     6721 gatggacgag ggacagatca aagagcgccc gagcgagagc cgcgtgcgag aagcggcggg
     6781 gctggacggg gtgcagatct tcgtggttgc ctgtccgaag gacctgacca tgtatcgcga
     6841 cgccgtcaag acggccggac ttgagggcac gattgtcgtg aaggacctga tcgaactcgt
     6901 ggaagaggcg acgagagaag aaaacgccgt cgcgccgatc gaggcggcga cgacaagtgc
     6961 ctgacaggca ctgtgcccgg gccggggata gccgattttc agaggaggcc agatcgatgg
     7021 ctgaacaagc acgccgtgac ggaacagaac gtgtgggcgt ttacgtctgc cactgcggca
     7081 gcaacatcgc cgccaccgtg gacgtggagg aagtcgcccg atgggccggt gaaagactgc
     7141 ccggcgttgt cgtttcacgg gactacaagt tcctgtgttc ttcgttgggg caggcgatga
     7201 ttcaagagga catcaaggaa cacggcctga cgcgtgtggt agtggcggcc tgctcgccgc
     7261 atctgcatga gaagacattt cgccgggcct gtgcaaacgc cggtctgaat gcgttcctcc
     7321 tgcaaatggc caacattcgc gagcacgtct cgtgggtgac caagcacaag gagacggcaa
     7381 cggagaaagt caaaacgctg gtggccgggg cggtggcgag ggtccagcat catcagccgc
     7441 ttgaacccat gccggtcccg gtaaacaagc acaccttggt cgtcggcggc ggcatcgcag
     7501 gcatccaagc ggccctggaa ctgggcgatg ccggctatcc ggtgtacctg ctcgaacggg
     7561 agccgagcat cggcggccac atggcgcaat atgacaaaac ctttccgacg cttgactgcg
     7621 ccgcctgcat cctgacgccg aagatgtccg aggtgggcca gcacgatcat atcaagctct
     7681 tgacctactc ggagctggag gaggtgtccg gctcgatcgg cgactttcac gttcgaatcc
     7741 gccggaaggc ccggcacgtc aaggaggccg actgcaacgg ctgcggactg tgcacggaga
     7801 aatgcccgcg caaggtcgtc gacgaggatt tcgaggccgg catgggatac cgcaaggcca
     7861 tttacgcgcc gtttccgcag gcggtgccga aggtgccggt gatcgacacc aagtcgtgca
     7921 tctggttcga gcgaggcaag tgccgggtct gcgagaaggt gtgtcccacg catgccatcg
     7981 acttcgatca agaggatgag atcctggaag tccgcgtcgg caacatcatt cttgccaccg
     8041 gttgggacct gttcgactgc cggcgcattc cgcagtacgg ctacggccgt ctggcgaacg
     8101 tctttaccag ccttgaattc gagcggatgt gcaacgcctc cgggccgacc ggcggcaaga
     8161 tcgtgcttcg cgacggcgtg accgagcccc aatcggtctg catcgtccac tgcgttggca
     8221 gcagggacca tcgctacaac acgtactgct caggcatctg ctgcatgctg tccctcaagc
     8281 tggggcacct ggtgatggag aagacggggg ccaaggtgta cgcgtgctac atcgatatgc
     8341 gccccaacaa aaaggactac gaggaattct actggcggct gctcgatgag gggatgcttt
     8401 ttgttcgcgg gaaggtggcc gaggtgaccg atattgcccg ctttccgaac gaggagggca
     8461 agctcatcgt tcaggtcgag gacaccttgc ttcggaagca gcggcgcatc ccggtcgata
     8521 tggtagtgct gatgggagcg atcgagcccc ggcgcgacgc gagggaaacc ggtttgaagg
     8581 tcggcatttc atgcagcacg gccggctggt acatcgagcg acacccgaaa cttgatccca
     8641 tcgcgacgat gaccgagggg attttcatcg ccggggcctg ccagggaccg aaggatatcc
     8701 ctgacgcggt cggtcaagga gccgccgcgg ccgctcgcgt gcagggcatg atcagcaagg
     8761 gcacggtgat gatcgagccg gtggtcgccg gcattgatga ggaccattgc tcgggatgcc
     8821 gcatctgcaa caacctgtgc ccgttcaacg ccattgactt catcgaagat gaaaaggtca
     8881 gcagaatcaa tgcggtgttg tgcaaagggt gcggaacttg cgtcgcggcc tgtccggcgg
     8941 gggccatttc cggggcccat ttcagtaacg cacagatcct ggccgagctt gaggggttgc
     9001 tctgcgacgc cgggggtgag aatgaattga tcgccggtgc gtcgggaaag ccgtcggaac
     9061 cggttgccgt gtaggagtcg tatcatggaa caagaaacgt tcgagccgaa gatcatcggt
     9121 ttcttctgca actggtgctc gtaccgggcg gccgacgggg cgggcaccgc aaggatcaag
     9181 cactctccca acgtccgcat cattcgcctg atgtgcagcg gccgggtgga catcgccttc
     9241 attctcaagg ctctctcgct gggtgccgac ggggttctgg tggccggctg ccatcccggc
     9301 gaatgccact acatcgagca gaactacaag accatccggc ggttcacgat gctcaggcac
     9361 acgttgcggg cattcgggat tgatgaaaga cgcgtccgcc tggtctgggc gtcggcaggc
     9421 gaggcgagtc atctggccga ggtgatcgac gagatggtcg aggacgtccg aacgctcggc
     9481 ccgctgaact ggcggcgaaa ctgggccgag gatggcgccc acctggacga cgtggagagg
     9541 atacttcatg agcacgagga agccatggag gtgtcgagat gagtgaaaaa ggcaaactcg
     9601 ccatttattg ggccgcctcg tgcggaggat gcgagatttc gattctcgcg ctgaacgaaa
     9661 agatactcga cgtcgcgaac gccttcgaca tcgttttctg tccgtgcatc atggacacca
     9721 aggcccgcga tgttgaaaag atggcggacg gcaccatcga cgtctgcctg ttcaacggcg
     9781 gcattcggac cagcgaacag gaatacatgg ccgggctgtt gcgacgcaag tcaaaggtcc
     9841 tggtggcgtt cggatcgtgc gcatacgagg gctgtatccc gggcctggcg aacctcacga
     9901 accgcaagga gatcttcgag acctgctaca tcgattgccc gtcggtggac aatccgaacg
     9961 aggcggttcc cgtcgcggac acgcgggtca acggctatcg cctgacgctg ccggtgtttt
    10021 atgacaccgt gcggacgctc gggcaaaccg tgaaggtgga ttactacctt cccggctgcc
    10081 ccccggaagc accaagaatc tgggacgccg tggcggcgat tcttgaggga aagctgccgc
    10141 cgccagggtc ggtaatcggc ccggaaacga cggtgtgcga cgaatgccag cgcaagcggg
    10201 aagagaagaa gatcaagcgg ttcaagcgga cttgggaggt tattcccgac gaggataagt
    10261 gcctcctgga gcaggggcta ctctgttgcg gaccggccac ccgggccggt tgcggggcct
    10321 tatgccccca ggtgaattcg ccgtgcatcg gatgttacgg tccgaacgcc ggcgtggagg
    10381 actttggagc caggctgatg agtgcgctcg catcggtgat cgacgcaacc gacccggacg
    10441 agattgatga gatcatccgc cagggtatcc ctgacccggt gggcactttc tatcggttcg
    10501 gtctgcccca cggctttctg cgacgggcgg cagcaatccc ggacgcgcaa cggcatgagt
    10561 tacccttgcc caaacctgca gaggaaagga gcaagtgacc atgacagcca agcacattgt
    10621 catcgacccg atcacgcgcc tcgaaggaca cggaaagatt gacatattcc ttgacgaaac
    10681 aggcgatgtt gccgatgcct tcctgcaaat ccccgagctt cgcggttttg aacgattctg
    10741 cgtggggcgt ccggccgagg acatgccgaa cctgacctcg cgcatctgcg gcgtctgccc
    10801 cgaggctcac cacatggccg cggccaaggc gctggatgct ttgtttcacg tggagcctcc
    10861 tccgaccggc aagaagctgc gtgagatgtt ctactccata ttttgcgcca cggaccacac
    10921 gactcacttc tacgccctcg gcggaccgga cttcgtcatg ggaccggatg cgcctaaaga
    10981 ggagcgcaac attctcgggg tcatcaagaa agtcggcatg gagatcgccg gcagagtcat
    11041 caagatgcgg gccgacgggc acggcctgat caagatgatc ggcgggcggg cggtacatcc
    11101 gaactgggcc gttccgggcg gcgtcagccg gggcattacc gaggagcaac gacgggagat
    11161 cgaacggctc gggcgcgacg ccatcgagtt cgctaagttt tccctgcagc tctttaacca
    11221 ggttgtcctc ggcaactccg agtatgtcaa gctcatccag agcgacgcat acacgcaccg
    11281 gacgtacaac atgggcaccg ttgacgccca gaatcgcgtg aacttctacg acggcatgat
    11341 tcgcatcgtc ggccctgacg ggcgggaaca cgccaagtac catccccgtg aatatcgcga
    11401 gtacatcgcc gagcacgtgg agccctggtc ctacctgaag tttcccttcc tcaagaagat
    11461 cggctggaag ggattcgtcg acggcgagga ctcgggcttg tacaaggcta cgccgctgtc
    11521 gcgactgaac gctgctgagg gcatggcgac cccgctggct cagcaggaat acgagcggat
    11581 gtacgacgcg ctgggcggaa agccggtgaa ccacacgctg gccactcact gggctcggtt
    11641 aatcgagctg ctgtatgcca cggagcgatg ggtcgagctc gccactgatc caggaatcac
    11701 gggaaaggaa tatcgcgtgc tgccgagcga gacgccggat gaaggcgtcg gcagcgtgga
    11761 ggccccgcgc ggcacgctga cgcatcatta ttggaccgac gaacgcggca tattgacgcg
    11821 ggtgaacctg atcgtgggca cgaccaacaa ctacgcaccg attcagctct cgatcaagac
    11881 ggctgctcga agtctgatcc gaaaaggaac ggcggtgagc gaggccctgc tcaacaagat
    11941 tgagatggcg tttcgggctt acgatccgtg cttcggttgt gccacacact gcctgcccgg
    12001 tcaaatgccg atgaccgtcg tcattcattc tgcggacggg agaacggttc atcgcctgtc
    12061 gcgaggggaa gggcggggga tcggagaacc ggttcgatga ctaacgccct tgcagcctgt
    12121 tcgcaggggc gggcatcggg cgaggggccc acactggtgc tgggcttggg aaaccctctt
    12181 ctcaccgacg acagtgtggg ccttcgggtg atcgaccatc tgcggccgca agttgcaggg
    12241 tggccgaacg tccaactgga tgaagactat tgcggcggtt tgggcgtcat ggaacgtatg
    12301 attggtttcg accgcgtcat tctcgtcgat gcgatctgca cgggtgggcg gcccggcgcg
    12361 gttcacgtcc tgaccgttcg ggacattccc acacggcaca gcggttcgtt tcatgacgcc
    12421 gatcttcgta cggctctggc cctcggtcgt caggcagggg cccgactgcc ggctgatgag
    12481 aacatccgca ttgtggcggt cgaagcggcg gaagtgctga cattcggtca acagtgcact
    12541 ccggccgtgc ggtcgggcat cagccgggcg gcggagagtg tgttgaaact cttgacagca
    12601 tggagttaaa tcaatggtac ctgtggagac tctgcgatcc ctggcttgcc tggccggcgt
    12661 gcccgtagac agcctcaagg cccttgcggc gatcgccgaa gaacgggatt tcaaggccgg
    12721 cgaggtgctc tggcacgagg gcgacccggt acgctggatg tgcgtcgtgc ggcagggcga
    12781 gatcgacgtc atctatcagt tgcagggtgg gaggcagtgc gtggtggaca cagttgtcgg
    12841 cggggagatc accggatggt ccgcctttgt cgaaccccat cgacatacgg ccacttgcgt
    12901 cgcccgcgca tccggtcgtg cgttgtatct cgaggccgcc cccatgcgag cgctctgcga
    12961 gaaggacagc cttcttggtt atcgcttgct caagcaggtc gccaagacgc tgagcagtcg
    13021 cttggagagc gccaggcttc aactggtagc aaccggctaa cacgtctgcc ggtcgcgttc
    13081 gacctgggcg tcggtcgtcg gccagcggcg gtgccggttg cgcagcccga tctcgcaacg
    13141 ggccagggag ggcgacagga gatcacaatc atgagccgcc gaattctttt gatcgatgac
    13201 gacccggaca tgcatctggt gatgaagatg atcctggagc cggagggtta cgaggtcacg
    13261 tgctgtcgaa cgggatcaca cggtctggag accatgcggc ggcagcgtcc cgatctcgtt
    13321 ctgctggaca tcatgcttgc ggacccttct gagggcctga gagtcgcctg cgagatgcga
    13381 cacgacgaga agctcaaggg gattccgatc atcatgatct cggccatcgg tgagcagatc
    13441 ggacaggatt actacagcga ggtgtgcccg ggcacggcgt cgggcgattt attcctggag
    13501 aagccgttcg acccgcccac ggtccgtgaa gcggtgaaat ggattctcga acggccggcc
    13561 gtggagtctt aacctgagaa caccggcgac attgaatcaa tcctggggtg cggcgggggc
    13621 ttctgattcc tcaagcacca cctgactggc gcgtgtattg cggacctgcg gttgacggat
    13681 gctgccggag ggcgagggga aggtgagcgt gaagctcgat ccctggccgg gacatgaact
    13741 cacgacgatc tgcccgcccg cctgctcgac gacctcacgg acgatggaaa ggcccaagcc
    13801 ggtgccggga attcctgccg cgaccacgtt tccacctcgt cgaaacgcat cgaaaaccgc
    13861 ctcgtggccc ggctcgccgt ccggcgacac cgcaaggtgg ggatcgattc ccataccggt
    13921 gtcggtgaca ttgacgagca cggccatgag ttcgccgttg gatcgctccg gcatcacggt
    13981 tacggtcacg ctacccgggc cgggcgtata cttgatggcg ttatcgatca ggttgccgat
    14041 acaatcacgc aggtctcgcg ggtcgacaca aaccgtcagt tcttcgtcgg acggcatgga
    14101 acagaccaac ttgatctgtt tgctgtctgc caggggtctg aaccgtccga acagttcgtt
    14161 gacggtttcc cggacatcgg accctgattg ccggtgacgg gaagggtctg cctcctgcac
    14221 tcgtgccagc gtgagcagct cggtgacctg ggcgatgcct tcctgacacc gatgaatgat
    14281 cttctcgcaa gttccggaga ccgcctcggg cggcaccaac cccgttcgaa ccagctcgac
    14341 catggtttga atcgccgcca aggggctttt gagctgatgt gccgccgtct gcatgaagcg
    14401 ggatcgacgg cgctgcaggt cctggatggc ccgttgtgat tgcttcagtg cggcattcgc
    14461 ctgttggagt tggcgctcct gggtctggaa gtggcccgcg atgtgcagtg taaagtagag
    14521 cgtgccgaaa atgccgcatg cgaccacgac cagagccgcc aggacgtatt ccggccgagc
    14581 atagagcccc acggaaacgg acggcaggaa gtcataatgg ggtgcgagcc acccgcgcca
    14641 ttcaccgatg accagcacgg cataaagcgc ccacgcccag atgccctgca gcacggcatg
    14701 ccatctcttg agaagcaagg agacaatgga catgtggaac aggtaaaaga tcgccaccgg
    14761 attctcgaca ccgccggtgt atcgcaaaat ggcggttaac agtaacaggt cggtggccac
    14821 ttgggcgttc gcgaaccatg cgagccgctc gatttgtgcg cgaagggcct tcggatcgcg
    14881 agtcacgtga cgaaacaggg atcgtgagac gatgagccac acaaggttga ccagggccag
    14941 cacgatcagg acgacggcca agtcaaaggg acgctgagta accggtgcga cgaagcgctc
    15001 caaggccaga gcggccacgg cgccggcgag gaagatccac ctcaggcgaa tgaaccagtg
    15061 cagacgaagc atggggacaa gggccaccgc gcgcggatcg ccttccaggt gggcaagcac
    15121 ttggatccaa tccgtctttt cgacttccgg actgcgcatg acgtcgtcct cgacaaccgg
    15181 tctgacttga atcaacatga ggactatgcg gcggcaacga gcttgggagc cgggactccc
    15241 ctgcccgacg tggcgcgtcg cttccagtgc ccgccccaat tgacatgccg accgcacagt
    15301 cggtcattat aaccgaagtc aagtcgattg ccttgctcaa tgggcgggga attgtgtgcc
    15361 accgatgcct ctgggcccgt ggttgctctt cagcttgcga cggtggcgac ggcctcctca
    15421 aacacattga gaccgacttc caactgggcc tggttgatac acagcggcgg gcaaaaacgg
    15481 atggtggtat cgccgcagcc ccacaaaagg aggccgcgcc ggaatgattc ggtgatgatg
    15541 cgttcgcgga gttgcgcggc cgaggtctgc gaacggcggg gcttgcggac atctactgcc
    15601 agcagaaaac ccataccccg aggcgagccg aggcagcgtc ggcgaccatc gatctgttcg
    15661 agcttggaga tgaagatcgg cgcaagccgg gcggcattcg caatcaggtg cttttcgagg
    15721 gcctcaaccg aagcgagcga ggcggcagaa ctcaccggat tgcccacgta gggagcggtg
    15781 accaagcctt cgtcactgct taacagggtt tcaggggcga tcagggcgtg aatcggcatg
    15841 ccgctgttca agccggttgc cgcgaggagt acgtcggcct gggtggcgaa atgttcacag
    15901 ccgaacatgc ggccggtacg acccatgccg gtgcgcgttt catcgatcac cagcaggacg
    15961 tcgtgctcgt cgcagactct gcggagttcc ggcaggaaat cggaactcgg gacatggtag
    16021 tcttcctcgg tgagcatcgg ctcggcgaag agcaccccga cctcgtcgcc caggccgctc
    16081 cggaaataat catgcaccgc cgcgacgttg ccatatggca ggctgtcaac aacccaggcc
    16141 tcgggtggaa tccggcgccg gtgacgtggc ctgccgaact cgagggtaat cgcggccaag
    16201 ccgcgattgt gcgaattacc gttgaacgtg acgatgcgcc ttcgggtgtt gcgtcttcga
    16261 gccattcgca acaccacttc gagcgattgc attcggctgt tcgagaagag cacgcggttt
    16321 tcaccacggg ttggggcgag ctgggcgagt ttgcgagcca gccccatcag cggctcgctg
    16381 gtgaattcgc cgctgcacgc tttggccatt cgttcgacct gctcctggat ggcccgcacg
    16441 accatcgggt gtgcgtgtcc ggcaacaaag gccaagcccc cggcggtgaa atcgaggtaa
    16501 cgattgccgt cgagatcctc aaccaccgaa cccctggcgc ggcggacgac gagcgggtag
    16561 ggccaaccgc tgggagaggc cgctacgcgc cggtcgtggg ccaaggccaa ggcggtcatg
    16621 ggaccgggcg gcgagaccac gatccacggg gcaggaccga tagccggttc ctgcggcggt
    16681 cgccccgata ctggtacatg aagagtcagc atcctaaacc catgccctcg ctttcgccag
    16741 aactctactt aagtatagca attccaagcc tctcgggcaa tcagatgggg cgttttctcg
    16801 tcgccggtgc aacccgaatg aggcacctga aaacctctta tcggctgaca aagccggttt
    16861 ccaagagaaa gcacccgatg tgaaaccggc tcacggactc ttacgcccgt gcgcggggtt
    16921 actcttcagt ccggaatgag aggcttgcct cgcggaggag actcggcacc gggcatgttt
    16981 cccggtccgg gcggttgaac agttccaact ggtcgcaagg gtatcgccgc gaggaaagta
    17041 ttgggcgact cttgtaagaa ggttgaagga acaaatggag aagtaaagct ttgttgtcga
    17101 gacctcgcat ggcggctgcc tgaaggctgc tgaccggcac ttttccgctg gtcctgagtt
    17161 gcgtcttgtc ttggacaccg cgattcaaga tcgtcggtca tccgtggcca gatcgaggct
    17221 tcagggccgc gccgtggccc tgggtattag gtccctgtac aatccgcact cacgcagacc
    17281 agatgcaatc agcagcgaag gagacggcat taatgaccgg tcgggcgaag atcacgatca
    17341 tcggtgcggg caacgtcgga gcgacctgcg ctcattgggc ggcagccaag gaattgggcg
    17401 acatcgtcct ggtggacatt gtcgaggggt tgccccaagg caaggcgctt gatctgcgcg
    17461 aggcctcgcc gatcgaaggg ttcgactgcg aaatcatggg gaccaaccgg tacgaggaaa
    17521 cggccgacag cgacgtgatc atcatcacct ccggcctggc ccgcaaaccg ggaatgagcc
    17581 gcgatgacct gatcgccaag aacaccgaga tcgtcggcag cgtagccaag caggccgcca
    17641 ggtacagccc ccaagcagtg gtgatcgtgg tgagcaaccc gctggatgca atggtatatg
    17701 ttgcgtggaa ggcatccggg ttcccgaccc atcgggtggt gggccaggcc ggtatcctgg
    17761 acactgcccg cttccggacc ttcattgcga tggaactcgg gtgcagcgtt gaggacatca
    17821 gtacgctgct gctcggcggc cacggcgatg atatggttcc gctgccaagc tgcactgccg
    17881 tcggcggcat cccggtcacg agcctgatcc ccaaggaccg gctggatgcc atcgtcgacc
    17941 ggacgcgcaa aggcggcggc gagattgtcg gcctgctgaa gaccggcagt gcgtattacg
    18001 cccctgcggc cgccagcgtg caaatggccg aagctatcat ccgcgacaag aaacgcgtgc
    18061 tgccgtgcgc ggcctactgc gacaaggaat acggcattgg cggctacttc gttggggtac
    18121 cctgtatcct gggcaagggc ggcgtcgaga aggtgctcga ggtgccgatg acgccggtcg
    18181 agaaggccgc attccaggtc agcatcgagc acgtgaagga acttgtggcc gtggttcaga
    18241 ggatcggggc gtgaagagag ggttcggggt tcagtcggct ggttccgcgc cggcggacca
    18301 ttcattcctt gtccggaggt tgtcaatgcg tctcttcgtt tcaatgatcg ccggggggtg
    18361 cgtgatcgct gcggtcggct gcacacccga gcaggtcaaa ccgggaatcg ccggcaagca
    18421 gcaaccggtg gccgagaagg tcgagatcaa gcgcgtggtt gattgccggt accttctgta
    18481 tctgcccgag aattacgaat ctcaggaccg ctggccgctg atactgttcc tgcacggagc
    18541 cggcgaacgc ggggacgata tcgaaaaggt caagatacac ggcccgccga agctcatcgc
    18601 acagggcaag cacttcccct ttattgtcgt ctcgccccaa tgcccgaacg ggcaatggtg
    18661 ggacatcgag gtgctcggtg ctttgctcga tcgcatcgag cgcgactaca aagtggatcg
    18721 cgaccgcatc tatgtaaccg gcctgagtat gggcggtttc ggcacgtggg cgttggcttg
    18781 tgcgcagccg gatcgtttcg cggcgatcgc cccgatttgc ggcggcggcg agccgaagct
    18841 ggccggacag atcgctcacg ttccggcgtg ggtcttccac ggcgatgctg ataacgtggt
    18901 gtctgtcagc agatcgaagg aaatggtcga ggctctgaag accgcaggcg gcgacgtgaa
    18961 attgaccgtc taccccgaag tgggccacga ttcatggacg cgcacctacg atgacccggc
    19021 actctatgac tggttcctgt cgcaccggcg gggcaagacc aggacgagag aatcgaagtg
    19081 agcaagccgc cggcaacgga gcattctctg ttctgcattc gccgttccgg attacccggc
    19141 gttgcggcgc gtaatgccga agcctacttc ttctgcggca gcactttcca cacaccggcc
    19201 ggcgagcgtt cgagaacgac gtatggtcgg tccctggtga cgatctcgat gacctggcca
    19261 tcaactctca ggaaggtccg cttgcgcgga gcgagcggca ggaagttgat gtggacctcg
    19321 atctgctgga cattgccggt ggtcacgttg acgtcattct gctcggggaa gaacccctcg
    19381 gcggttgcgg tctgattttc gtcattgagc ttgagaatcc gcagccagcc gggttgcttg
    19441 tcgcttgggt tggacacata caatttgacc ctgtacgggg ggttggaatc gtaggtcgag
    19501 acaggctgac tcgcgaccgg ccggctctcg gcaggcttgg tggcgaccgg acgtgtctgg
    19561 gcgagcggct gctcaaccgg cagggctaag cccttcggtt cgggactctt cttgttcggt
    19621 gattccttgg gacagccggc cccggagacc agaaagatca ccatcagggc cggcataacc
    19681 gcatgtcgaa tgcgtgggtt catgggtcaa ctcctggatt gggccgcggg agcatcttat
    19741 ccgcctcact attggacacc aaatagttcg aacggttgcg gagcttcggc gattgtgcta
    19801 cggtcctccg actggccgag catccggcaa gaagaggagg actggctcgc ccagaagtcg
    19861 ctacgacgag tgttgtgctt gaggcagatc ggacgacgga ctgctgagaa aggccggtgc
    19921 aagtcccccc ttttcgccaa gggagcacaa accatgcggc aaggtgagac ggcgatccaa
    19981 gcgacgacca ttctggcggt gcggcgggga gagcagacgg ccatcggtgg cgacgggcag
    20041 gttacgatcg gccaaaccgc tctgaaacac gacgccaaca aaatccgccg gctgagcggc
    20101 ggcaaggtga tctgcggctt tgccgggtcc gcggccgacg cgttcgcgct tctggatcga
    20161 ttcgagaaga agctcgatga gttcaagggt aatgttcgcc gggcagcgat cgaactggcc
    20221 aaggattggc gcaccgaccg cgtccttcgc caccttgaat cgatgctggc cgtcgtggac
    20281 agccagacct ctctgatcat cggaggaagc ggcgacgtga tcgagccgac ggagggcgtc
    20341 atcgggatcg gctcgggcgg catgtatgcc gtcgccgccg cgagggccct gctcaaacac
    20401 tcgaatctcc ccgccgccga catcgtccgc gaagccctgg cgatcgccgc ggatatctgc
    20461 gtgtatacca atcacaacat catcgtcgag acgcttcctt gaaaccgcgc gggacatagg
    20521 ccaagttggt cggtttcttc cgcagcacgt ggccaaggga agtcatcatc cgagatgact
    20581 gctcggccgg tcaggtcaga tcagtcaaca gactgctcgc tgaccacgat ctccagcggc
    20641 tggccggtgg tggccgggtg cgtcacatgg atcgtgtcga cgaactgagc atccgtcagg
    20701 gtcaggtggt acgttccggg ctcgggcgag atggtcagtg acagcgtggc caggactgtc
    20761 tgaccggcgg gtagcggttc tcttgtcacg ccgccgatgc cggccgtggt tgtgaaccac
    20821 tggactgact cctgcgactc gatcggaggc tcggccgtcg gggtggcgat ggggccgttg
    20881 atcgtcggca agtctgcgtc cttccggcca aggatgtggg ggggcgggga ggttgggcga
    20941 ggcgttcggg gtggatcgga tgacggggaa ccggccacgg ttttccttaa gtgtttaatc
    21001 g
//
LOCUS       DAOTPW010000085        20924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2595_length_20924_cov_5.296852, whole genome shotgun sequence.
ACCESSION   DAOTPW010000085 DAOTPW010000000
VERSION     DAOTPW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20924
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2595_length_20924_cov_5.296852"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="P5316_09500"
     CDS             <1..278
                     /locus_tag="P5316_09500"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85234.1"
                     /translation="MLADFDKIVKEYMERTGRPMNHARNFLDCVRSRKPTVANAEVMY
                     HSMATVHAANICMWLKRDMKYDPARKEFADADANGFRSRPMRAPWVI"
     gene            complement(518..2053)
                     /gene="rho"
                     /locus_tag="P5316_09505"
     CDS             complement(518..2053)
                     /gene="rho"
                     /locus_tag="P5316_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRR85235.1"
                     /translation="MARASDKPRSSANRNGGRRRTVKDDPPVAAAPEKDEEVLFGADI
                     DDTSSEDSSQDTVAAEEPIPTTSTIDAETHAKYEEVKRGELHIRDLQKLSVDALHEIA
                     RQEGLSEYTGLTKSELIFRILKERIRQNGLMYGEGVLEILPDGFGFLRNPEYNYLPCP
                     DDIYVSPSQIRRFGLRTGHIVAGQIRPPKESERYFALLRVEAINLEDPDRVTEKTNFE
                     DLTPLHPGPKDPKTARLGVDGRLRFETTPDEINMRVVDLVTPIGKGQRMLIVAPPRTG
                     KTVLMQKMANAIAANHPECYVIILLIDERPEEVTEMQRATKAEVISSTFDEPASRHVQ
                     VAEMVIEKAKRMVEYGHDVVILLDSITRLARAYNTEVPHSGKILTGGVDANALQKPKR
                     FFGAARNIEEGGSLTIIGTALVDTGSKMDEVIFEEFKGTGNSELHLDRRLVDRRTWPA
                     IDINASGTRKEELLLDPNELELVYKLRRVLSDMNVVEAMELLRSRLLKVRTNAEFLMT
                     MNF"
     gene            complement(2175..2780)
                     /gene="coaE"
                     /locus_tag="P5316_09510"
     CDS             complement(2175..2780)
                     /gene="coaE"
                     /locus_tag="P5316_09510"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRR85236.1"
                     /translation="MKPGKPIIGLTGGIGSGKSTVAGFLRDLGAGVIDADRLSHEELD
                     SPEVLRQIRLWWGDDVVTPDGRADRSAIRRIVSADAGQRKRLEDLVHPRIDRRRRELA
                     EQYRIDPSVRAIVLDAPLLHEVGLDRECDCLVFVDADRAVRLDRVRKRGWGPQDLERF
                     EESQLPLDTKRDRADYRVVNNSDVADLRRQVEEVFSRILAG"
     gene            complement(3358..3738)
                     /locus_tag="P5316_09515"
     CDS             complement(3358..3738)
                     /locus_tag="P5316_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRR85237.1"
                     /translation="MRIPRILALTDVNMASPPDQAEELVAFYTQLVGLDHLLDESDER
                     RQVFRGDRRRGPRIVVWLRSPLREEDRRRVLIQVASLRDYVERLSEERIFWQEIRGWG
                     YYDRRVYVFDPAGNLVELVCYHPF"
     gene            complement(3707..6163)
                     /locus_tag="P5316_09520"
     CDS             complement(3707..6163)
                     /locus_tag="P5316_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRR85238.1"
                     /translation="MSSEDDRGSGISADDTCVPFRVSTWCLCLRTFCPALVVEPVGGY
                     KPHSMFARLGQLIGRRPGALITIWALVVAGSALWAFRTDPPPPPKTDSLLPADCAYNR
                     ALELIHREFPALAARSRVVIVGYRDTGLEPADLTWLGTAARQAAQAAASVTGVDSCER
                     VLSPDVPYLKPRLVSPDGQAAMALVNLPTNFISPITARAVNAIDEALGRMPRPVGLTV
                     EMTDSAVGGRDYALKAEEALDRTTLVTIVAVLAILTLVYRSPVGVVVPLVSIGASVFL
                     AFVVLAALAKGGWEVSDMERIFAVVLIFGAGVDYALFWISRYRESLQTKIEYGPAAVL
                     ATRFAGPAILAGAATTICGLSTLILTDLGPTRSAGKVLAIVLTIGLLAALTLAPPLCR
                     ALRGAFFWPVGAAGGSSIGQRHLWPRLARIVADKPVLCLLAGTLLLGLPALYSLRLPA
                     RFDSLSELPPDSPSARGFELMTRHFKKSQLYAGPLMIEFEHPLALPDARQLSQSISDR
                     IVPISGVDDVYSLGAPLGRKQKAGMPPQLAGLLSQLAAEFYISKSSEVLRLEILIDHL
                     PFSPEAMNIVEQAASISREEAAEYADRYGQARILGSGLTAYVLSVRDVVQADQRRVMI
                     LATLVIGLIVLGLIRHLRLTVFMLLATWLTFGATVTFSDLFFTRVMGTYGLDWKVRLI
                     VFVIVVAVGQDYNIFLAARLFQELPQAGRAEAARRAIVSTGAVISNCGLIMAATLGSL
                     WAGKLTLLQQAGFSLALGVLIDTFFVRPILIPSFFLVVMRSRDQGQAEVSPVATAGQT
                     AGGFAVQARTYADPENTGSD"
     gene            6320..6541
                     /locus_tag="P5316_09525"
     CDS             6320..6541
                     /locus_tag="P5316_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85239.1"
                     /translation="MKNTHVRDTFTLWVFWLTVGVLLVSALSGILCGVSLRFQRPRRV
                     TRGLAIISVIAFILGLAGCTILCTLTNWR"
     gene            6740..7519
                     /locus_tag="P5316_09530"
     CDS             6740..7519
                     /locus_tag="P5316_09530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85240.1"
                     /translation="MQNGREAIIEYFNRVAPEYGVKHGADMPGGQYSFGQLYRRILSP
                     YFKPGMEVLELGCGNGASTEMLASYGVKLVATDICEKMLKVAAARGIANTTFRCLDIM
                     EIAGAQDLGGFDVIVSFNAFSYLPDKARVLHDLSRLLRPQGRLIILDMNALSPVYWFC
                     VLMGRNEMRQWWGAIKEMTPGNLRFLFQRCGYAVELIRTLNFVPHATQGRVFRALRAM
                     NPLLNATPLIRKLAMRVLVVATRSEAHNGSKQGKVDEGMAR"
     gene            7476..8678
                     /locus_tag="P5316_09535"
     CDS             7476..8678
                     /locus_tag="P5316_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRR85241.1"
                     /translation="MAASRVKWTRVWLDENPSDEFYLPVSLPEIVARPPGQPLLWADD
                     GRGRRLIGSRATAQGPVHWAFEMGEWLRGCLNMRGREPRRPIYTRVPGFDVYRVPARL
                     RAVIFRSVFSRTGKKPPTDAPDRSSGVQRLAAAMGSLQDGGALSDLRSPVWPDRKRAA
                     LIFTADVDSEYIFASRDNRIRRMLEEHGFRAAWFFLTGRYKLDHGHLDDLRAAGHEIG
                     WHGHNHDHRMAFVGRRARLRRIDHARAFFERYQVCGMRADNFLWSRALFDDLRGVLKY
                     DTSMVDAFPFTERGRGCATGLPFRMDSGLWQLPTTVTPDCFLSDSWPIENWLELQERQ
                     VRRQFSLHGVIHLLVHPEPAVSLDPRRLELFGRVLGLLDSLRDEVWHGLPMDLFALTS
                     RDGQKRSI"
     gene            9032..11812
                     /locus_tag="P5316_09540"
     CDS             9032..11812
                     /locus_tag="P5316_09540"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRR85242.1"
                     /translation="MRLSLVVIVPSLCAWASAEPTSSRSFGTAKQGSSLYPPDVVQRL
                     RDNVAEDPWAAGVRDSLIQAAQPWMDRSDDELWALMFGPGITRSWMVWSNGHCPACGQ
                     SVPMYNWKCDALNRPWKIQCPHCQEFFPKNDFLRFFKSGLDKKGVFDPARADRSLLFN
                     TEHPDPADPKHKFGVDDGEGYVEGDKRWRFAGAYEIYGQWKQAVLGGIRTLSGAHLLT
                     GDPVYAHKAAVMLDRVADLYPLFDFKEQAVVYERRLGSSGYVSVWHDACEETRELVMA
                     YDMIFEASRDDTDLVRFLSGKAREIGLENPKSSFANIQRNIEDRILRDPLAQRPKITS
                     NYPRTEITVAIIYAVLGGGDSKAAFDRTVDAMLEKATAVDGVTGEKGLSGYAVFTIQG
                     LAMFLAEFSKADPGLLPRLLERHPRLKETYRFHIDTHCLGRYYPTCGDAGSFASAGGG
                     YAGMPLTTSFARPTSFPSWTCVAPSCHSLLWDFYCCTGDVAYVQTLYRANGDRLDGLP
                     FDLYAKDPAALRKAIGEVIEKEGAEVRLRSVNKQQWHLAILRSGQGRDARAAWLDYDS
                     GGGHGHADGMNLGLYAKGLDLMPDLGYPPVQFGGWDSPRARWYTMTAAHNTVTVDGAN
                     TSPGAGQTTLWADGSMLHAIRVAGPALNAGRRFERTVALIDVSAADCYVVDIFRAAGG
                     KEHTRFMHSHFAEVSSRSLNLQPEPDFGHGTQMRNYRMDKQPRPERHVDWIIQDHYKY
                     LPEGQQVRVRCTDLTNDAQVGLIEGWIVAGLYDESQEQWIPRMAVRRQAPGNGGLEST
                     FVAVIEPYETRAIASAVRRLPLLSEQDEPLSDSHVALVIQLADGRRDVVIARDPEDKT
                     AAGMIRSADFDVRTDAELVLVRLTAEGSVEYAIACHGTRLTSKDWTFAIPAGADFAEW
                     PSTTGMDARVRQ"
     gene            11809..13980
                     /locus_tag="P5316_09545"
     CDS             11809..13980
                     /locus_tag="P5316_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRR85243.1"
                     /translation="MKSVSGSRCRTVINAVALVMVACSIVRVCRAWEGHDWNEWKATT
                     TWTKPELKTSQTGRKELVELLAVEGTPLRRVDEIRGWEQRRTEIAAAIQAVLGRPSGL
                     TPPRPEAKVLGEETLPDHIRRHVRIRTEADDWIPAYLLLPKDVPQGRLPTMICLHQTV
                     AQGKDEPCGIKGSGDLSLALQLVRRGYVCIAPDVIGFGERIPAGSAPYHNSIAFYRKH
                     PGWSFMGKMIWDVSRIVDWLETQPYVDPLQIGCIGHSHGAYGSIFAGAFEMRISAVIA
                     SCGFTTLRSDPDPDRWSHKTALIPQLGIYLPDVASIPFDWQQICALVAPRPLYVWYTT
                     RDSIFPNTDNLEALFKDVRSVYGLYGAANDLTWSFEESGHEFPTAAREAAYRWLDQRF
                     FAVGDLHAQPADLKEWQNRREMLKRVIRRTIGAPSTRPVSPEVETISQETLPKYERRL
                     IEYSVDQGERIRAFLCVPSERSEPTPAVLVFHQTAAEGKREAAGLAGDKSLAFGMELA
                     ERGYVTLSPDSITAGERIDGYGAFDTRGHYLRHPDMSAMGKMLHDAQRALDVLVAVEG
                     VDRNRIGAIGHSLGAEETLMLAAFDDRVAAAVASCGYATFRAETAESRLRWARDHWFS
                     YMPGLRPVFQAGRLPHWDWDDVLRLAAPRAVFQYNTRDDEIFIESESAFDAGETARAI
                     WRLYGKEDLPANVLRPGRHAIAEQDKPEMYEWLDRQLKESR"
     gene            complement(14101..15783)
                     /gene="thiC"
                     /locus_tag="P5316_09550"
                     /pseudo
     CDS             complement(14101..15783)
                     /gene="thiC"
                     /locus_tag="P5316_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851680.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     regulatory      complement(15883..16040)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16276..17784
                     /locus_tag="P5316_09555"
     CDS             16276..17784
                     /locus_tag="P5316_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRR85244.1"
                     /translation="MLRFQVHHNGRPVSDMDLAGTYLVGSDGVPLRAELEFRDSQIVC
                     AKRADGPAGLAVLWPIPGCGAILSETSRLMDRDQPYNLLLELVRGRLTRINQKREDWG
                     LFDFEGVEQVAEQVDKARDLFIAALCADSPVEQSKVAEEALKLAFVAGEQLCQFHADL
                     FLGRRKQTPGFTKRVFGCTVDLHNNSDEYRERFKETFDFAYIPVPWRLVEPKQQTREW
                     QLIDNWIEWLAKSRLPVRMGPLVSLDAEHIPDWLSQYEDDYESVRSAIVEHVRRTVER
                     YGNYVYCWDAVSGIHAENAFNFTFEQIMDLTRVTVSLVKQLAAKSSQVAIDLVAPWGE
                     YYARNQRTIPPMFYADMISQSGVGFDGIAAQFVFGAPRDGMFVRDMFQISDKLDRLGN
                     LGKPVHITAVQVPSKMIGEKGSLGGGCWLKPWDEAVQAKWARGFYTVALSKPFVDSIT
                     WIDLADRPSNRLIPFGGLLKEDLSPKPAYRVLKDFRAELVATARRPPGQRVR"
     gene            17991..18287
                     /locus_tag="P5316_09560"
     CDS             17991..18287
                     /locus_tag="P5316_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRR85245.1"
                     /translation="MTLLPLVPGAFPAAAPAEVDSRVDPNTATWAELASLPGIGEVLA
                     KRIVEYRQGKAGRPDKVVFRQPADLEAVRGIGPKTVAKLAKHLKFPETATVPGP"
     gene            18437..18646
                     /locus_tag="P5316_09565"
     CDS             18437..18646
                     /locus_tag="P5316_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85246.1"
                     /translation="MRRWILLALLGLLALAQSGCGGDGVAYTRRERIQRMKRTMEWDA
                     KQYADDVDLFWLNDQNLRTSKWSIP"
     gene            complement(18643..20049)
                     /locus_tag="P5316_09570"
     CDS             complement(18643..20049)
                     /locus_tag="P5316_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR85247.1"
                     /translation="MLFNGLWRRIIRMTVGLLVLTILLAGPAMAAESSRPNVLFLFTD
                     DQRPDGIAALGNSAIKTPVMDSLARQGLVFRNAYIFGSNVGAVCQPSRNMLLSGRAYF
                     RWNGPYAPADQPNFPASMKQAGYFTYHHGKKGNNAVLIQKLFDVDKYVDESQDRKSGE
                     PGRIIVDEAIAFLRNRPVDRPFFMYLAFGNPHDPRVADRKYLDLYQRDKIPLPRNYMP
                     LHPFDNGEMVIRDELLAPWPRTEDEIRRQLHEYYAVISGLDHNLGRLLDAVRQLGLFD
                     NTIIIFSSDNGLALGSHGLMGKQSLYDHSAKVPLIIVGPGVPRGSSDALVYLMDIFPT
                     ICEMTGTAVPDGLDGKSFKPVIDEKVKSVRDSVFMSYGNCQRGIRDERFKLIRYPLID
                     KTQLFDLQADPDELHDLADDTGQKERIEKMMVQLAEWQKRLGDKAPLTVAEPKNPKFT
                     PPTGEALNKLLEQWKIKP"
     gene            20571..>20924
                     /locus_tag="P5316_09575"
     CDS             20571..>20924
                     /locus_tag="P5316_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85248.1"
                     /translation="MIRKIITLRGESAWFRQHVEFDAVDLPGSPRAPQLTLERVVLLD
                     EPLPEPVEFTMPGWQSYPVFTESLFFGVEFPVASAMVKDGRIVLSHAPGRVMTLPTSY
                     RSREAVVGVCPPGRVR"
BASE COUNT         4017 a         6233 c         6587 g         4087 t
ORIGIN      
        1 ccatgctcgc ggacttcgac aagatcgtga aggagtacat ggagcggacc ggccgcccca
       61 tgaaccacgc acggaacttc cttgattgcg tccgatcgcg aaaaccgacg gtggccaacg
      121 ccgaggtcat gtatcactcg atggccacgg tgcatgccgc gaatatctgc atgtggctca
      181 agcgggacat gaaatacgac ccggcccgga aagagttcgc cgatgcggac gcgaacggct
      241 ttcgttcccg gcccatgcga gcgccctggg tgatttgagc atcgcgtcga atcacgataa
      301 agcttgctgc ccgcgcatcg aatgctcaac aactgattca ttcgccggac aggcgatggg
      361 tgggagaatc aacccggacc gcgatggcac gttctgggtc tgcaacgcat cggcagtcgc
      421 atgcgcaagc cgttgccaga caggcaatca gtgcaacctg cgcagaggta gacccgcgcg
      481 ggtcccatcc tctttctcgt gtgaagtcat attggcgtca gaagttcatg gtcatgagga
      541 attccgcgtt ggtccggacc ttcagcaggc ggctgcggag cagttccatg gcctcgacca
      601 cgttcatgtc gctcaagacg cggcgaagct tgtagaccaa ttcgagctcg ttcggatcga
      661 gcagcagttc ttccttgcgg gtgccgctgg cattgatgtc gatggccggc caggtgcggc
      721 gatcaacgag gcggcggtcc agatgcaact cgctgttgcc ggtgcccttg aactcctcga
      781 agatcacttc atccatcttg ctgccggtat cgaccagggc ggtaccgatg atggtcagcg
      841 aaccgccttc ctcgatgtta cgggcagcgc cgaaaaaccg cttgggcttc tgcagggcgt
      901 tggcgtcgac accgccggtc aggatcttgc cggagtgcgg gacttcggtg ttgtaagccc
      961 gggccaggcg ggtgatcgag tcgagcagga tcacgacgtc atgaccgtat tcgaccatgc
     1021 gcttggcctt ctcgatgacc atctcggcca cctgcacgtg ccggctggca ggctcatcga
     1081 aggtcgagga gatcacctcg gccttggtcg cccgctgcat ttcggtcact tcctcggggc
     1141 gctcgtcgat cagcaggatg atcacatagc actcggggtg gttggcggcg atggcgttgg
     1201 ccatcttttg cataaggacg gttttgccgg tgcgcggcgg ggccacgatc agcatacgct
     1261 ggcccttgcc gatcggcgtc accagatcga cgacgcgcat gttgatttcg tccggcgtgg
     1321 tctcgaagcg cagacgacca tccacgccga gcctggcggt cttcggatcc ttggggccgg
     1381 ggtgcagggg ggtcaggtcc tcgaaattgg ttttctcggt gacgcgatcg ggatcctcaa
     1441 ggttgatcgc ctcgacacga agcagggcga aataccgctc cgactccttg ggcgggcgga
     1501 tctggccggc cacgatgtgc ccggtacgca ggccgaagcg tctgatctgc gacgggctga
     1561 cataaatgtc gtccgggcag ggaaggtagt tgtactcggg gttgcggagg aaaccgaagc
     1621 cgtcgggcag gatttccagc acgccctcgc cgtacatcag gccgttctgg cggatccgct
     1681 ccttgaggat gcggaagatc aattccgact tggtcagacc tgtatactcc gagaggcctt
     1741 cctgcctggc gatttcatgc agggcgtcca cgctgagctt ctggaggtct ctgatgtgca
     1801 gttcgccccg cttgacctcc tcgtacttgg cgtgtgtctc ggcgtcgata gtgctggtgg
     1861 tgggaatcgg ctcctcggct gcgacggtat cctgagacga gtcctcggag gaggtgtcgt
     1921 cgatgtcggc cccaaacaag acctcctcgt ctttttccgg ggccgccgct accggcgggt
     1981 cgtcctttac ggttctacgg cgtccgccgt tgcggttggc cgaacttctc ggcttgtcgc
     2041 tggctctcgc catactggca ttcctcctgc tacttactgg ctagggtact cacccctgca
     2101 aaagtcattt caagttcaag ctgtcgtacc ggcatcacac ccggaaaccg ctatggtttg
     2161 gagtgcgggg tctgctaacc ggcgagtatc cgggaaaaaa cctcttccac ctgcctgcgg
     2221 aggtcggcta cgtccgagtt gttgaccacc ctgtaatcgg cacgatctct tttcgtgtcc
     2281 agtggcaact gcgattcttc gaatcgctcc agatcctgtg ggccccagcc ccgcttccgg
     2341 acccgatcca ggcggacggc ccgatccgca tccacgaaaa cgaggcaatc acattcccgg
     2401 tcaaggccta cctcgtgcaa cagaggagca tccaggacga ttgcccgcac ggacggatcg
     2461 atccggtatt gctccgccaa ctctcgccgc cgccggtcga ttcgcggatg aaccaggtcc
     2521 tccagtcgtt tcctttgacc cgcgtcggca ctcacgattc ggcggattgc cgagcggtcg
     2581 gcacgcccgt cgggcgtcac cacgtcgtcg ccccaccaca ggcggatttg ccggaggacc
     2641 tcaggcgaat cgagctcctc atgagagagc cggtcggcgt cgatcacccc ggctcccaga
     2701 tcccgaagaa accccgccac agtgctcttg cccgacccta tgccgccggt aagaccgatg
     2761 atcggcttgc cgggcttcac aatccgtcgt tcccttcgat ctatgatgcc aacactgctg
     2821 ggagagacat cttcaggcgg tcggcctcgt gcccacatat aacacggcac gtgatccgac
     2881 accgtccttg agcttctcac caccccattg accccgttgt tcctccatcg tcagcatccc
     2941 gtttgacggt cttgacggaa aacccgcaac gggcgcggcc tctcgcaaca agcatcctcg
     3001 tcaggagtag tactcaccta cggtccaaaa gggaaaccga cgagtgatgg agatgatacc
     3061 tgctgctccc gagtcttgcg tcacccggga gtgccgccaa aactcaaaca aggtactcac
     3121 agtataacgg caagttcggc tgttgtcatc tcttatataa atgattctgc cgcgttttgc
     3181 aagcggtggg gtaatcctgc cccctgttgc gccgctgcag tatgcgccac aatctcgtaa
     3241 cttgttgtca gtaagtatgt tacattagcc tcttccgtcg cggctggtgc gcgttttgcg
     3301 cgcatgtttc tggatcgcag agcacttggg ttgcggcccg caaaaagacg gtccgcctta
     3361 gaacggatga tagcacacca actcgaccaa gttgcctgcg gggtcgaaaa catacacccg
     3421 tcgatcataa tagccccaac cccgaatctc ctgccagaag atccgctcct cgctgaggcg
     3481 ctcgacgtaa tcgcgcaggg aagcaacttg gatgagaact cgccggcggt cctcttctct
     3541 caaaggactg cgcagccaca ccacgatgcg cggtcctcgt cggcgatccc cgcggaagac
     3601 ctggcgacgt tcgtctgact catcgagcag atgatccagg cccaccagtt gtgtgtagaa
     3661 ggcgaccagc tcctcggcct ggtccggcgg cgaggccatg ttcacatcag tcagagccag
     3721 tattctcggg atccgcatac gtacgcgcct gcaccgcgaa gcctcccgcg gtctgaccgg
     3781 cagtggcgac cggcgagacc tccgcctgtc cttgatcccg ggaacgcatg acgaccagga
     3841 agaaactcgg gatcagaatc ggtcttacaa agaacgtgtc gatcagcact ccgagggcca
     3901 ggctgaaccc ggcctgctgc aacagcgtca gcttgccggc ccacaaagat ccgagcgtgg
     3961 cggccatgat gagcccgcaa ttgctgatca ccgcgcccgt gctcacgatc gcccgccgag
     4021 ccgcttcagc ccgccccgcc tgcggaagtt cctggaacag ccgagccgcc agaaagatat
     4081 tataatcctg ccccaccgcc accacgatga caaacacgat cagccgaact ttccagtcca
     4141 gaccgtatgt tcccattacg cgagtgaaga acaggtcgct gaaggtgacg gttgccccaa
     4201 aggtcagcca cgtcgccaac agcatgaaca cggtcaaccg caggtggcga atgagaccca
     4261 ggacgataag cccgatcacc agcgtggcca gaatcataac ccgccgctgg tcggcttgga
     4321 ccacgtcgcg aaccgagagc acataggccg tcaatcccga gccaagaatc cgggcctgac
     4381 cgtacctgtc tgcatattca gcggcttcct cccggcttat cgacgcggcc tgttccacga
     4441 tgttcatcgc ctcgggcgag aaaggcaggt gatcgatgag gatttccagc cgcagcacct
     4501 ccgaggactt cgagatgtag aactccgcgg caagctgtga cagaaggccc gccagctgtg
     4561 ggggcattcc ggccttctgt ttgcgaccca gaggtgcacc gagcgagtag acatcatcga
     4621 cgccgctgat tgggacgatc cggtcggaga tggactggct gagctgccgc gcgtccggca
     4681 atgccagcgg atgttcgaac tcgatcatca aagggccggc atagagctgt gatttcttga
     4741 aatgccgggt catcaactcg aatccgcggg cggacggcga atcaggcggc aattcgctca
     4801 atgaatcgaa tcgggcaggc agccgcaatg aatacaacgc cggcaacccc aggagcagtg
     4861 tgccggccag caaacagagc acgggtttgt cggccacgat cctagccagt ctgggccaca
     4921 ggtgccgctg gccgatgctg gagcctccgg ccgcaccgac cggccagaag aaggcacccc
     4981 gcaacgctct gcacaagggc ggggccaacg tcagggcggc aagcaggccg atggtcagca
     5041 cgatggcaag aaccttgccg gcgctgcgag ttggtcccag atcagtgaga atcagggttg
     5101 acaggccgca gatggtggtg gctgctcccg ccagaatggc cggcccggca aagcgcgtgg
     5161 ccaggacggc ggcgggtccg tactcgatct tcgtctggag cgactcgcga taccgcgaga
     5221 tccagaacag ggcatagtcc acgccggcac caaagataag taccaccgcg aagatccgtt
     5281 ccatgtcgga gacttcccag ccccctttgg ccagggcggc cagcaccacg aaggccagga
     5341 acacgctcgc cccaatggac acaagcggca caaccacgcc caccggcgag cgatacacga
     5401 gagtcagaat cgccagaacg gcgacgatcg tgaccaaggt ggtgcggtcc agggcctcct
     5461 cggctttcag agcgtagtca cggccgccaa ccgcgctgtc agtcatctcg acagtcaggc
     5521 ccacgggacg cggcatccgg ccaagggctt cgtcgatcgc attcaccgcc cgggcagtga
     5581 tggggctgat gaagttcgtg ggcaggttga ccaaggccat ggcggcctga ccgtcggggc
     5641 tgaccaaccg cggcttgagg tagggcacat ctggagacag aacgcgctcg caagagtcaa
     5701 cgccggtgac cgacgccgcg gcttgggccg cttgccgggc ggctgtgccc agccacgtga
     5761 ggtcggccgg ttccaggccg gtatcgcggt aacctacgat caccacccga ctgcgggccg
     5821 ccagagcggg gaattccctg tggatcagtt ccagggcccg gttgtaagca caatcggcgg
     5881 gcagaagcga gtcggtcttc ggtggcggcg gcgggtcggt gcgaaatgcc cacagggcac
     5941 tgcccgcgac gaccagcgcc cagatggtaa tcagggcacc cggtcgccga ccaatcaatt
     6001 gtcctaatct ggcaaacatc gagtgtggtt tgtagccgcc aacgggctca actacaagcg
     6061 cggggcagaa cgtccgcaga cacaggcacc acgttgaaac gcgaaacgga acacaggtat
     6121 catctgcaga tatgccggag ccgcgatcgt cttccgagct catggcacac tcttacggag
     6181 atctgaggaa gccggagatg ccggaagaag cactgagcaa acatgagaag ggagccgtcg
     6241 acttcatttc gcgaagggct gcgtcgccct caccgggatt gggattcccg gtatcatcgg
     6301 cacgggcacc atcctgagct tgaaaaacac acacgttcgc gacacgttca cactatgggt
     6361 gttttggctg accgtcggtg tgctgctggt ctctgcgctg agcggcattc tctgtggcgt
     6421 ttctctgcgg tttcaaaggc cgcgaagggt cactcgcggg ttggccatca taagtgtcat
     6481 cgccttcata ctggggctgg ccggctgcac catcctctgc acgctcacga actggagata
     6541 gacaacccgg acaaacaagg acttcccgtc ggaatgccgt gccctcctga gcactgtcgc
     6601 gaagcctccg tgccggactc gcgcgatgcg accggacaga cctcttgcgg cgcttgagac
     6661 gggagatggg gcatggtaga ttgccgccgg caggtgcagg ttctccgcgt ttggccggcc
     6721 ttcaggaagg aattgtctcg tgcagaacgg tcgcgaggcc atcatcgagt acttcaatcg
     6781 ggttgctccg gaatacggcg tgaagcacgg tgccgacatg cccggagggc agtacagctt
     6841 cggccagctt tatcgacgga ttctgtcgcc gtacttcaag cccgggatgg aggtgcttga
     6901 actgggctgc ggcaacgggg cgtccaccga gatgctggcc agttatgggg tgaagcttgt
     6961 cgccaccgat atctgcgaaa aaatgcttaa ggtcgccgcc gcacgaggca ttgccaacac
     7021 caccttccgt tgcctcgaca tcatggagat tgccggggcg caggacctcg gcggttttga
     7081 tgttatcgtt tcattcaatg ccttcagcta tcttcccgac aaggcgaggg tcctgcatga
     7141 cctgagtcgc ttattgagac cgcaaggacg cctgatcatt ctggatatga atgctctcag
     7201 cccggtgtac tggttttgcg ttctgatggg gcggaacgag atgcggcagt ggtggggagc
     7261 catcaaggag atgacccccg gcaatctcag atttctgttt cagcggtgtg ggtacgccgt
     7321 ggaactcatt cgcacgctca actttgttcc gcatgccacg cagggacggg tattcagggc
     7381 gctgagagcg atgaaccctc tcttgaacgc gaccccactg atacgcaagc tggcgatgag
     7441 agttctcgtg gtggccacca ggtccgaggc gcacaatggc agcaagcagg gtaaagtgga
     7501 cgagggtatg gctcgatgag aacccttctg acgagttcta tctgccggtg tcattgccgg
     7561 agatcgttgc ccgtcccccc ggccaacccc tgttgtgggc cgacgacggt cggggtcgtc
     7621 ggctgatcgg cagccgggcc acagcccagg gtccggttca ttgggcgttt gagatgggcg
     7681 agtggctcag gggctgcctt aacatgcgcg ggcgggagcc gcgccggccc atctacacac
     7741 gggttccggg ttttgatgtt tacagggttc ctgcccgttt gcgtgccgtc atctttcgga
     7801 gcgttttcag ccggacaggc aagaagcctc ccactgatgc cccggatcgc agttcgggtg
     7861 tgcaacgtct ggctgctgcc atgggctcgc tccaggacgg cggggcgctc agcgacctcc
     7921 ggtcgccggt ctggccggat cggaagcgcg cggctctgat cttcaccgcg gatgtggaca
     7981 gcgaatacat cttcgcaagc cgcgacaacc ggatccgaag gatgctggag gaacacgggt
     8041 tccgcgccgc ttggttcttc ctgaccggca ggtacaaact ggaccacggc cacctcgatg
     8101 acctgagggc cgccggtcac gaaatcggat ggcatggcca caatcatgat catcgtatgg
     8161 ccttcgttgg ccggcgggca cgcctgcgcc gcatcgacca cgccagagcc ttcttcgagc
     8221 gctaccaggt ctgcgggatg cgcgccgaca atttcctctg gtcgcgagcc ctcttcgacg
     8281 acttgagggg cgtgctcaaa tacgacacca gcatggtgga cgccttccca ttcaccgaac
     8341 gggggcgcgg gtgtgcaacc ggtctgccat tccgcatgga tagcggcttg tggcagttgc
     8401 ccacgaccgt cacccctgat tgtttcctgt ctgatagttg gccgatcgag aactggctgg
     8461 aactccagga gcggcaggtc agacgtcagt tcagtcttca cggcgtgatt cacctgctgg
     8521 ttcacccaga accggcggtg agcctcgacc cccgcaggct tgagctcttc ggccgggtac
     8581 tgggtctgct ggacagtctg cgcgacgagg tgtggcatgg actgcccatg gacctgttcg
     8641 cgctgacgtc tcgcgacggg cagaaaagga gcatatagct cttactgaga tacggcttaa
     8701 gaaagccctg tcgagaacag gccgcgtcag catgaatcct gccggaatgg ggcgattgtg
     8761 atgccggcgt gcaggaacgg catggttgct gcggatcatc gagtcggaga ttgtgcgcca
     8821 actggggatg gggcaggtgg gcgccaatcg cgtcgggttc gggaccggcg caacatggaa
     8881 caacccggcg agcaacgtgg ccggattcga tcacgcggaa cggagcgcat ggcagcgaga
     8941 agccatcgcg gcacatggag aatgtgagtc ccggagattc ggagggcgag gcggtagtct
     9001 cggcccagca gtggtaacgt ggagtctgat catgcgacta tccttggtcg tgatcgtgcc
     9061 atccttatgt gcctgggcat cggcggaacc gacgtcgagt cggtctttcg ggacagccaa
     9121 gcagggcagt agcctgtacc ctcccgacgt cgtgcagcgg cttcgcgaca acgtggccga
     9181 ggatccgtgg gcggccggcg tgcgtgacag cctgattcag gccgcccaac cctggatgga
     9241 caggtcggat gatgagctct gggctctgat gttcggcccg ggaatcacgc gctcctggat
     9301 ggtctggtcc aacggccatt gcccggcctg cggccagagc gtcccgatgt acaactggaa
     9361 gtgcgacgcc ctgaaccggc cgtggaaaat ccagtgccca cattgccagg agttctttcc
     9421 gaaaaacgac ttcctcaggt tcttcaagtc aggcttggac aagaaaggcg tcttcgatcc
     9481 ggctcgtgcc gaccgctcgc tactgttcaa caccgagcat ccggaccccg ccgaccccaa
     9541 gcacaagttc ggcgtggacg acggcgaagg atacgtcgag ggcgataaac gctggcggtt
     9601 cgccggagcg tatgagatct acggccaatg gaagcaggcg gttctgggcg gcatccgcac
     9661 gctaagcggg gcacaccttc tgaccggcga tccggtgtac gctcacaagg ccgcggtgat
     9721 gctcgaccgc gtagccgatc tgtatccgct gttcgacttc aaggagcagg cggtggtcta
     9781 cgaacgccgg ctgggttcca gcggctacgt ctccgtctgg cacgacgcct gcgaagagac
     9841 tcgcgagctg gtcatggcgt atgacatgat ctttgaggcc tcaagagatg ataccgatct
     9901 ggtcaggttc ctgtccggca aggcccgcga gatcgggctg gagaatccca agagcagctt
     9961 tgccaacatc cagcggaaca tcgaggaccg cattctgcga gatcctctcg cccagcgtcc
    10021 gaaaataact tccaactatc cgcgcaccga gatcaccgtt gccatcattt atgcagtgct
    10081 gggcggaggc gacagcaagg ccgcattcga ccggaccgtg gacgcgatgc tcgaaaaggc
    10141 cacggccgtc gacggcgtta caggcgagaa gggcttgtcc ggctacgcgg tcttcactat
    10201 tcaggggctg gccatgttcc tcgcggagtt cagcaaggcc gaccccggtc ttctgccgcg
    10261 gctactcgaa cggcatcccc gcctgaagga aacctaccgc ttccacatcg atacgcactg
    10321 tctcgggcgg tattacccga catgcggcga cgcgggcagt ttcgcgtcgg ccggcggggg
    10381 ttacgcaggc atgccgctca caacgtcgtt tgcgcgtccg acctctttcc cttcgtggac
    10441 gtgtgtggcc ccgtcgtgtc acagcctgtt gtgggatttc tactgttgca cgggcgacgt
    10501 cgcctacgtt caaacgcttt accgggcgaa cggagatcgc ctcgacggtc tgccttttga
    10561 cctgtacgcc aaggatccgg ctgcactccg aaaggcgatc ggcgaggtca tcgagaagga
    10621 aggagcggag gttcgcctgc gaagcgtgaa caagcagcag tggcatctgg cgattcttcg
    10681 ctcgggacaa ggccgcgacg cccgagcggc gtggctggac tacgattccg gcggaggtca
    10741 cggccatgcg gacggcatga acctgggcct ctacgccaag ggactcgact tgatgcctga
    10801 tttgggctat ccgccggttc agttcggcgg atgggactcg ccgcgcgccc gatggtacac
    10861 gatgacggcc gcccacaaca cggtgaccgt tgacggcgcg aatacttcgc ccggggccgg
    10921 acagacgacg ctctgggccg acgggagtat gttacacgcg attcgggtgg ccggtccggc
    10981 gctgaacgcc ggccgacgat tcgagcgaac cgtggccctg atcgacgtga gcgccgcaga
    11041 ctgctacgtc gtcgatatct tccgagcggc cggcggcaag gagcacactc gcttcatgca
    11101 cagtcatttc gccgaggtga gcagtagaag cctgaatcta cagccggagc ccgatttcgg
    11161 ccacggcacg caaatgcgca actaccggat ggacaagcag ccccggccag agcgtcatgt
    11221 tgattggatc attcaggacc attacaagta cctgcccgag ggtcagcagg ttcgcgtccg
    11281 ctgcaccgat ttgaccaacg atgcacaggt gggcctcatc gaaggctgga ttgtcgccgg
    11341 gctgtacgac gaatcgcagg agcaatggat cccccggatg gccgttcgca ggcaggcccc
    11401 cggaaacgga ggcctcgaat cgacatttgt ggcagtcatc gaaccctacg agacccgggc
    11461 gatcgcgtcc gccgtcaggc ggctgccgtt gttgtctgaa caagatgagc cgctctcaga
    11521 ctcgcatgtc gcccttgtga ttcagcttgc cgacggacgg cgcgacgttg tgattgctcg
    11581 ggatccagag gacaagaccg ccgcaggcat gattcggtcg gctgatttcg atgttcgcac
    11641 ggatgctgaa ctcgtactcg ttcgcctcac agccgagggt agtgtcgagt atgccatcgc
    11701 atgccacggg acccgactga cctcgaagga ttggacgttc gccatcccag ccggcgcgga
    11761 ttttgctgag tggccaagca ccacaggtat ggacgcgagg gtcaggcaat gaaatcggtt
    11821 tccggttccc gctgcagaac agtgattaac gcggtcgctc ttgtgatggt tgcctgctcg
    11881 atcgtgcgcg tgtgccgcgc ttgggaaggc cacgattgga acgagtggaa ggcaaccacg
    11941 acctggacga agcctgaact caagaccagc cagacgggtc gaaaggagct ggtcgagctg
    12001 ctggcggtcg agggcacgcc attgagacga gtcgacgaga ttcgcggctg ggagcagcgg
    12061 cggacggaga ttgctgctgc gatccaggcc gttttgggac gaccatcggg cctgacgcca
    12121 ccgcggccgg aggcaaaggt gctgggtgag gaaacgctgc cggatcacat ccgccggcat
    12181 gtacgcattc gtaccgaggc cgatgactgg attcctgcat acctgttgct acccaaagac
    12241 gtgccccagg gacgtttacc gacgatgatc tgcctgcacc aaacggttgc ccagggaaag
    12301 gacgagccgt gtggcatcaa gggcagtggc gatctgtcgc tggctctcca gcttgttcgc
    12361 cgaggctacg tctgcatcgc cccggacgtc atcggctttg gcgagcggat tccggccggc
    12421 agcgcccctt atcacaacag cattgccttt tatcgcaagc atccgggctg gtcgttcatg
    12481 ggcaagatga tctgggacgt ctcgcggatc gtggactggc tggagacgca gccgtacgtc
    12541 gatcccctgc agatcggctg cattggccac tcgcacggag cgtacggctc gatctttgcg
    12601 ggagcgttcg agatgcgcat ttcggcggtc atcgccagtt gcggtttcac cacgcttcgt
    12661 agcgaccctg atcccgatcg atggtcgcac aagaccgccc tcatcccgca gctcgggata
    12721 taccttcctg atgtggcctc aattcccttc gactggcagc agatctgcgc gcttgtagct
    12781 ccacggccgc tgtacgtgtg gtacaccacc cgggactcga tttttccgaa caccgacaac
    12841 ctggaggcgc tgttcaagga tgttcgctcg gtgtacggac tgtacggggc ggcaaatgat
    12901 ctgacctgga gctttgagga aagcgggcac gagtttccca ccgcagcccg cgaagcggca
    12961 taccggtggc tcgaccaacg attcttcgcc gtgggagact tgcatgcaca gccggccgat
    13021 ctcaaggaat ggcagaaccg tcgggagatg ctcaagcgcg taattcggcg aaccatcggg
    13081 gcgccttcaa cacggccggt gtcacccgag gtggaaacga tctcccagga aaccctgccg
    13141 aagtacgagc ggcggctcat cgaatactcg gttgatcagg gtgagcggat acgggcgttt
    13201 ctgtgcgtcc cctccgaacg atccgaaccg acgccggccg tactggtgtt ccatcagacc
    13261 gccgccgagg gcaaacgcga agccgcaggg ctggcgggcg acaagtctct tgccttcggt
    13321 atggagctgg cggagcgagg ctacgtcaca ctttcgccgg actcgatcac cgcgggcgag
    13381 cggatcgacg gctacggtgc gttcgatacc cgcggccatt acctgcggca tcccgacatg
    13441 tcggcgatgg gcaagatgct tcatgacgca caacgggctc tggacgtgct tgtggccgtc
    13501 gagggggtgg atcgaaaccg catcggggcg atcgggcatt cgctgggggc ggaggagacg
    13561 ctcatgctgg cggccttcga cgatcgcgtg gcggctgcgg ttgccagttg cggttatgcg
    13621 actttcagag cagaaaccgc ggagagccgt ctccgctggg ccagggacca ctggttcagc
    13681 tacatgcccg gattgcgacc ggtctttcag gcgggtcggt tgccgcactg ggattgggac
    13741 gacgtcttgc gtctggcagc tccgcgggcg gttttccagt acaacacccg ggacgatgag
    13801 atcttcatcg agtccgagag cgcgttcgat gcaggcgaga cggcccgcgc gatatggcgc
    13861 ctttacggaa aagaggattt gccggccaac gttctaagac cgggcagaca cgcgattgcc
    13921 gaacaggaca agccggagat gtatgaatgg cttgaccggc agttgaaaga aagtcgataa
    13981 ccgtctcaca cgcggagatg cagccccgtg acagacccgc ccgccgactc acgggtcggc
    14041 cgctgcagtc gcacccgatc gtgaaacgcc ctgggttggc gcgggtgacg ttgccttctt
    14101 gcggatgtcc cgattcatct ccatgctgca gaacttggga ccgcacatgc tgcagaagtg
    14161 ccctgtgcgg gcttcgtctt tcgacaggct ttccgcgtgg aacaatcggg ctttctcggg
    14221 atcgagcgac aggttgaact ggtcctccca tcgaaaatca aaacgggcct gggccaacag
    14281 gttgtcgcgg tactgtgctc cggggtgacc cttggccaga tcggccgcgt gggcggcgat
    14341 tttgtaggcc aacacgcctt cgcggacgtc gtcgcgattg ggcaagccga gatgctcttt
    14401 gggggtgaca tagcacagca tggccgcacc gtaccagccg atcatggctg ccccgatggc
    14461 actggccaag tggtcgtagc cggcggcgat atcggagatc accgggccga gcgtgtagaa
    14521 cggggcctcg tcgcaccact caagctggcg ggtcacgttc tcctggatca tgtgcatggg
    14581 aacgtgcccg gggccttcgt tcatgatctg gacgttgtgt tcccatgcgc ggcgggtcag
    14641 ctcgccctga gtcttcagtt cggcgaactg ggcctcgtcg ttggcgtcgg ccagacaccc
    14701 gggccgcagg ccgtcgccaa tggagatggc cacgtcgtaa gcggccacga tttcgcaaat
    14761 ctcgtcccag tgaaggtacg cgaaattctc ggcgtcgtga gccagggacc acttggcgtg
    14821 aatcgagcct ccgcgagaaa cgatgccgca aacacgacct gtggtcagtg ggatgtgctc
    14881 gcgaagaaga gccgcgtgaa tagtgaagta gtcaacaccc tgttcagcct gggcgatcag
    14941 cgtgtcgcgg aaaacctccc aggtcaactc ctcgacgcga ccgtcgactt tctcaagtgc
    15001 ctggtagatc ggtaccgttc caatcggcac cgggctgttg cgaacgatcc actcgcgtgt
    15061 ttcgtcgatg tccgctcccg tagaaaggtc catgacggtg tcggcgcccc agcgggtcgc
    15121 ccagatcatc ttttcgatct cttcctgaat gcaggatgca acggcggaat ttccgatgtt
    15181 ggcgttgatc ttcaccagaa aattgcggcc gatgatcatc ggctcggact ctgggtggtt
    15241 gacgttggcc ggaatgatgg cccggcctcg ggcaacctcg tcgcggacga actcgggcgt
    15301 aatgacggaa gggatacttg ccccccacgg ccggccggga tgctgggccg cgatcggacg
    15361 gctaagcgcc tcgtttgcct ggttctcccg gatggcgacg aattccatct cgggcgtgat
    15421 gattccccgg cgggcgtagt acatctgggt tacgcatcgg ccgggtttgg cacgtcgaac
    15481 cgaatggccg gctcggaggc cggccactac ggtgtcgccg cgggcatcaa cccaccgaga
    15541 gcgaataggt ggcaggcctc tgccggcgtc gataaccatg tcgggatcgc tgtagggtcc
    15601 ggaagtgtcg tagacggtaa tgaacctggc tgggccgcca caaagccgga tctcgcgcat
    15661 gggcacacgg acgtcggggt ggatcgtgcc ggaaagatag actttcctgg agccgggaag
    15721 cgggccccgc gaagaaaaaa ggggcggttt acccgcatcc atcacagggg tggtcgcttt
    15781 catcggtgcc tatcctcaag cgatctgggc agggtcgtgc ccgacaccgc atcgcgaatc
    15841 aaatctggaa gggggagtcg ctgaccgcaa gtctggtcgc agtctggagt gctctcgcct
    15901 tccctacgca ggtctccccg ccggcagccg cgcaagcctg agcggacggc gggtttcact
    15961 gatcaggttc caagggtacg gcgaccgctc gtgtcgccca tctcaggctg gccgcgtgcc
    16021 agtcaccccc ggcgaactaa tggagtgtaa ggaacctcgg acgggatgac aagtccaagg
    16081 cttgtgttgg cacttggaat accttaggat tccgatggat atgggggtaa tggtggacgg
    16141 ctgcaaaaag tggaggtgga catctggcgc tcaacaaggt ggtaccggtt gaccgatagg
    16201 gtgtcattgc ccatcggggc ttttttcgga ttcatacttg tcataagctt ataccataac
    16261 taccggtaac tactgatgtt aagattccag gtgcaccaca atggtcggcc agtcagcgac
    16321 atggatctcg ccggcactta ccttgttggc agtgacgggg ttccgctgcg cgccgaattg
    16381 gagtttcgcg actcacaaat tgtttgcgcg aagcgggccg acggaccggc agggctggcc
    16441 gtgctttggc ctattcccgg ttgcggggcc atactcagcg agaccagccg tctcatggat
    16501 cgcgaccagc cctataacct cctgcttgag ctggtccgcg ggcggttgac gcgcatcaac
    16561 cagaaacgcg aggattgggg tctgtttgac ttcgaagggg tcgagcaggt tgccgagcag
    16621 gtcgacaagg cgcgggatct gttcatcgcg gcgctgtgcg cggattcgcc cgtggagcaa
    16681 tcgaaggtgg cggaggaggc gctgaaactg gctttcgttg ccggggagca gctctgtcag
    16741 tttcatgccg acttgtttct cggccgccgc aagcaaactc cggggttcac caagcgcgtt
    16801 ttcggctgca cggtagacct gcacaacaac tccgatgaat atcgtgagcg attcaaagag
    16861 accttcgact ttgcttacat tccggtgcct tggcggctgg tggagcccaa gcagcagacc
    16921 cgcgaatggc aattgatcga caattggatt gaatggttgg cgaaatcgcg actgccggtc
    16981 cgaatgggcc ccctcgtgag tcttgatgcg gaacatattc ccgactggct gagccagtac
    17041 gaagacgact acgagtctgt ccgcagtgca attgtcgagc acgttcggcg aaccgtcgaa
    17101 cggtatggga actacgtgta ttgctgggac gcggtcagcg gcattcatgc ggagaacgcg
    17161 ttcaatttca cctttgagca gatcatggac ctgacccggg tgaccgtgtc gctggtgaaa
    17221 cagttggcag ccaagtcgtc gcaggtggct atcgatctgg tcgcaccttg gggcgagtac
    17281 tatgctcgta accagcggac gattccgccc atgttctacg ccgatatgat cagccagagc
    17341 ggcgtcggtt ttgacggcat tgccgctcag tttgtttttg gggcgccgcg ggacgggatg
    17401 tttgttcgag acatgttcca gatctcggat aaacttgatc gtcttggcaa tctgggcaaa
    17461 cctgttcata tcactgctgt tcaggtaccc tcgaagatga ttggcgagaa gggcagcctg
    17521 ggcggaggat gctggctcaa gccttgggac gaagcggttc aggcaaaatg ggcgaggggc
    17581 ttctacacgg ttgccctaag caagccgttc gtcgacagca ttacatggat agatcttgcc
    17641 gaccggccat caaaccgcct gattccgttc ggcggacttc tcaaagagga cttatcgccg
    17701 aaaccggcat acagagtact taaggacttc agagcggaat tggtggccac ggcgaggcgg
    17761 cccccgggcc agcgggtccg ctgacgaacc gccatttcgg ggaaattgac ccaacgcggt
    17821 tgtgacacgt gcctcagcca gattgctcaa ctgacagcaa gtccgcgtcg gcccctctgc
    17881 cggcctacgt gatctgctat cttctgttcg gagtggtact tgcgatggct tgggcgactc
    17941 ggcgcggttg ccttccctac ctggaagccg gtcaggaggg gagcgcatcg atgaccttgc
    18001 tcccgcttgt tcccggggca tttcctgcgg ctgcacctgc tgaggtggac tccagggttg
    18061 acccgaacac tgccacttgg gcagagttgg ccagcctgcc ggggatcgga gaggttcttg
    18121 ccaagcggat tgtcgagtat cgccaaggca aggccggtcg gcccgacaag gtcgtttttc
    18181 ggcaacccgc agacctggaa gcggttcgcg ggattggtcc caagaccgtt gccaagctgg
    18241 ccaagcacct gaagtttccc gaaacggcca ccgtaccagg gccgtgaccg cctcgggccg
    18301 gcgactcggc cgcccttggc cccgcaggtt gacagcagtg gcgaccggtc tctagaatgc
    18361 gtgtcgctgt ggacatgcct ttccgcggac ttgggggtcc gcggccgata attaaccccc
    18421 atgggcgagg ttcaccatgc gacgttggat actcttggca ctgctgggtc ttcttgccct
    18481 tgctcagtcc ggatgcggcg gggacggcgt agcgtacaca cgacgggaac ggattcaacg
    18541 gatgaagcgc acgatggaat gggatgccaa gcagtacgca gatgacgtcg atctcttctg
    18601 gcttaacgac cagaatctcc gcacgtccaa gtggagcatt ccctaaggtt tgatcttcca
    18661 ctgctcaagc agcttattca gcgcttcgcc cgtcggcggc gtgaacttgg ggttctttgg
    18721 ctcggccacc gtgagggggg ccttgtcgcc gagacgcttt tgccattcgg ccagttggac
    18781 catcatcttc tcgattcgct ccttctgccc cgtgtcgtcg gccaagtcgt gtaactcgtc
    18841 ggggtcggcc tgcagatcga aaagctgcgt cttgtcaatg agggggtagc ggatcagctt
    18901 gaatcgttcg tcgcggatcc cgcgctggca gttgccgtat gacatgaaaa cagaatcgcg
    18961 gacgctcttt accttttcat cgatcaccgg cttgaaactc ttgccgtcaa ggccgtcggg
    19021 aaccgccgta cccgtcattt cgcagatcgt ggggaaaatg tccatcaggt agacgagggc
    19081 gtcgctgctg ccgcgaggaa cgcccggtcc gacgatgata agcggcacct tggcgctgtg
    19141 atcgtacaag ctctgcttgc ccatcaaacc gtggctgccg agagccaagc cgttgtcgga
    19201 cgagaagata atgatggtgt tgtcgaacaa gccaagctgc ctgacggcat cgagcagccg
    19261 gcccaagttg tggtccaacc cgctaatgac ggcatagtac tcgtggagtt ggcgccggat
    19321 ttcgtcctcg gttcgtggcc aaggggccag gagctcgtcc cgaatcacca tttcgccgtt
    19381 gtcgaagggg tgaagcggca tgtagtttct tggcagaggg atcttgtcgc gctggtagag
    19441 atcaaggtat ttccggtcgg ccacacgggg gtcgtggggg tttccgaaag ccaggtacat
    19501 gaaaaatggt ctgtctacgg gccggttcct gaggaaggcg atggcctcgt caacgataat
    19561 cctgccgggt tcaccgctct tgcgatcctg gctctcgtcg acgtacttgt cgacgtcaaa
    19621 cagcttctga atgagcacgg cgttgttgcc cttcttgccg tgatgataag tgaagtagcc
    19681 cgcctgcttc atggacgcgg ggaaattcgg ctggtcggca ggggcatagg gcccattcca
    19741 tcggaagtat gcccgaccgc tgagcagcat gttgcggctc ggctgacaga cggcgccgac
    19801 gttgctgccg aagatatagg cgttgcggaa cacgagccct tgccgggcca gcgagtccat
    19861 gacaggcgtc ttgatcgcag aattgccgag cgcggcaata ccgtccggcc gctggtcgtc
    19921 tgtgaacaga aagagcacgt tagggcggct gctttcggcg gccatcgccg ggccggcgag
    19981 aagaatcgta agcacgagca aaccgacggt catccgaatg atacggcgcc acaggccatt
    20041 gaacaacatg tcagaatcct ccgtcgcgaa tggggtccat aataccacaa gccgcacgat
    20101 acgtcctgct cctgagagct ctcaaacaac ctgatttgac catgcttcct gccgggacga
    20161 aaatgtgttg catgtcgaat gacaccgcgt gaagtggcga ttcctacaga gtcggcctgt
    20221 aacttggcga gcgagcgatg aacagaatcc ggcgtggagt tctgcgcctt ttgtggtgcg
    20281 ggtcgggcta tctggcggct ctggtcgctg caggttgcgc ccgcccgctc gagaacgcgc
    20341 acatgcgcct gctgttcaac gccgatgggc cggcagaacc actccaagtc atcaaccggc
    20401 gagcgggtcg atctttcgcc atatcgacaa ccgcgcctct cctacagttc acagaggggc
    20461 aagccggcgg tcctcaggtc cggccgactc gcgcggccgc tcgcttcttc ccgggcggtt
    20521 cgtcactcgt catcgaatac ccgcggcaac gagttggtct cggggagata ttgatacgga
    20581 agatcattac cctgcgcggt gaatccgcgt ggttccgcca gcatgttgag ttcgatgctg
    20641 ttgatcttcc aggcagcccc agggcacccc agttgacgct ggaacgagtc gtcctgctcg
    20701 acgaacccct gcccgagccg gtggagttca cgatgccggg atggcagagc taccccgtct
    20761 tcaccgagag cctctttttc ggcgtggagt tcccggtggc cagtgccatg gtcaaggacg
    20821 ggcgcatcgt tctgtcgcat gcccctggta gagtcatgac cctgccgacc tcctatcgtt
    20881 cgcgcgaggc ggtcgtcggt gtctgtccgc ccggacgggt gcga
//
LOCUS       DAOTPW010000086        20881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2603_length_20881_cov_4.897772, whole genome shotgun sequence.
ACCESSION   DAOTPW010000086 DAOTPW010000000
VERSION     DAOTPW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20881
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2603_length_20881_cov_4.897772"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="P5316_09580"
     CDS             complement(<1..1038)
                     /locus_tag="P5316_09580"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRR85249.1"
                     /translation="MRFSRTSQNAVLLVRSSTEGTEGLIRSLESRGGFDVVWVQSAAE
                     AVTRVAADLCPLVLYDLADAANVAICPGPTAQSGCDASQSGTLLSQGLQVFRQIKQQS
                     PHSQIVVLIAQGADVTTCCQAVVSGATSFVEWAPCMAVDQVVDTLKQALDRYEASVRE
                     GEDLRSRAIFDEAGIAGRSQIMAKLLFQAKRAALVSDAPVLVCGESGTGKQLLAEAIH
                     RMDPKRRNYPFLTVNCAAITGTLAESALFGHRKGAFTGATQDRPGYFLAADKGTLLLD
                     EISELEKPLQPKLLRVLQEGKILPVGDDIERQVNVRVIAACNRPLPILVQTGEFRLDL
                     YQRLNVISLTMP"
     gene            complement(1335..2237)
                     /locus_tag="P5316_09585"
     CDS             complement(1335..2237)
                     /locus_tag="P5316_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85250.1"
                     /translation="MKNGFRTSGAKGRRRRLSLLDSAIGKDAFTLIEVLVVVAIIALL
                     LAMLLPGLSRARRQAATVVCLSNLHQMLTTTQAYTAVYSGFYPPSHDTVTAPAANGDS
                     DRQIDYGWDITRITDLMTGKVRVLPGLIWQGKTIEAIQQCPGFAGPAMWADDRYTGYN
                     YNSSYIGAFRKRKEKTVSLGPAAPAQAVSWSVVVRPARQDEIRMPSLCALFGDGEYVA
                     GANKFMRSPWGSEQGARDTWVNSGRGAGTQGYRHTGRTNVAFADGHARPWNRRHTETY
                     DLEKPYIGDNNGFLSPDNSLYDLK"
     gene            complement(2271..3392)
                     /locus_tag="P5316_09590"
     CDS             complement(2271..3392)
                     /locus_tag="P5316_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85251.1"
                     /translation="MCRWYAILGAVVTAAGTAVNGYEYNRSDFAFYVDLPGNYVEGDG
                     VPWDFISFDDFNDPFAAGDRPTIDTTGDNDFASGTGSAANPVPVVPVYQPFRAFEIVS
                     IGKGGHLILEFDHHVLDDPLNPCGVDFIIFGNSNMIIDGISRWRNGDPNQTLLQGPIA
                     KEPATVSVSQDGVTWYTFVDGPFADDFAPTLGRIYDPAGPVSSLPGNQWWGQPTDPTY
                     PLSPDLHAPLGAVPEPTTFSAWTLAGLAMKYGYSAGGTGFDLAALQNLPVGPHGLRWF
                     RYVRIDNPIGSGCVPEIDAVADVAPRLVPDLDCDSDVDGADVDRFEECASGPGVARLA
                     QQCVRADFDQDGDVDQTDFGFLQRCITGPDVLLDWDCMN"
     gene            complement(3409..3936)
                     /locus_tag="P5316_09595"
     CDS             complement(3409..3936)
                     /locus_tag="P5316_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85252.1"
                     /translation="MNVTRAVAIRVFLTGFLGASLLGTAMAEGSPIPVGYGNSHAEVT
                     VEFVDGTFYVFEVSFDGQTTGLGLLDVIETSTTLTTIRNDFGWGVFVDGISYEGHSNV
                     GYGGGENWWHYWIKEPGGNWQSPSYGASSRTVIDGCSDAWIYGRAGMPIPEPVTVLLA
                     ALATTLLLKRSVRVG"
     regulatory      complement(4117..4364)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4554..5870
                     /locus_tag="P5316_09600"
     CDS             4554..5870
                     /locus_tag="P5316_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85253.1"
                     /translation="MSNTKHHPVTVTTRRDFLKFSAAAAAGATTLSVAGGAHAAGNQV
                     IRIGMIGCGGRCTGAADESMQAGPDVKLTAMCDVFKDKIEKSRESLKSKHPEQVQVDD
                     AHCFVGLDGYKKVIDSVDVVLIACASKFHPMYAEAAIKAGKHVFVEKPHGIDALGVHR
                     MKAVCELAKDKNLSILSGLQSRWHAGWQETIKRIHDGAIGEIVAIQCMFLRAPYVVVE
                     REPGLSELEYQFRNWYHFCWLSGDDVPQSLVHNVDRASWALKEQLPTWAFGLGGRSAS
                     FGEKYGDMFDHHTVVYEYAGGARVYALCRTQENTYNNYSDIIMGTKGTCYLGECRIEG
                     EKKWEFAGQHNNPGAAEQKALIEAVRNDKPINSGYHMNNSTMMGVMGQVACYTGKPVK
                     WEEIWKSDFSFGPPPEKVTMDMEPPTKPEAGGDYPIPIPGQTRLLG"
     gene            complement(6260..7474)
                     /locus_tag="P5316_09605"
     CDS             complement(6260..7474)
                     /locus_tag="P5316_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRR85254.1"
                     /translation="MAVIGQRNDGISRRSIPSNPHTDGLSQGELIAGGYFTTAGGVTA
                     NGVASWNGTHWQPLGNGIYGTVWALTVYNGELIAGGVFPSASGVSVNHIARWNGSVWR
                     PLGSGMSTENYRPVRAMAVYNGELVVGGHLSTAGGVTCNNIARWNGSAWQPLGSGTEG
                     IVLSLTVYNGELVAGGGFTTAGGVSANRIARWNGTDWQPMGSGLNDTVHALTVHNDEL
                     IAGGSFTTAGGQVSAYWARWKASLPVIDQQPTSQVSPLGQAVQFNVLASATTGTLSYQ
                     WRKDGVALTDGGNISGATTATLTVDPVGLGDAGSYDVVVSDDCASIAGNAALLTIMFP
                     PGDFDLDGDVDQDDFGRFQACLSGDTVPQGLPECRPAHMDGDSDVDKNDVALFLQCLS
                     GPDVLADVDCAP"
     gene            complement(7384..8352)
                     /locus_tag="P5316_09610"
     CDS             complement(7384..8352)
                     /locus_tag="P5316_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85255.1"
                     /translation="MAPLGTGTDGDVCALTIYGSDLVIGGSFVSAGGSYVNYLARWNG
                     SSWSSIGGGMGSDVYALAVHNGDLIVGGKFKTTAAGFHVYAIARWNGSTWQQMDLGMY
                     ASPMTDPTVYALAVYNGELVAGGNFDRAGNVSFWDTRCIARWDGSTWKPLGSGLGIAW
                     PDPGVRALTVHGGGLIAGGGFISAGGAGTARVARWNGSEWQSLGGGISGGVLALTVYN
                     GDLIAGGDFITGGAVGADSVARWDGVAWQPLGGGIGQTPNVDALASYGNELIVGSRMY
                     RVDNDFIAEVLGWNGTSWRSLGSGMTGSAADPYPQIHTLTVCLRAN"
     gene            complement(8307..8894)
                     /locus_tag="P5316_09615"
     CDS             complement(8307..8894)
                     /locus_tag="P5316_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85256.1"
                     /translation="MHKLFSSFSGVILVVMLLSPSAGSAVAQDCQGQWLPGEGLPGVN
                     GTVYAATSWDPDGAGPLDELLVVGGSFNVAGTNLASNIASWNGSTWQPLGDGTNGTVR
                     ALAVWNGELIAGGEFATAGGATVNCIARWNGSTWQPLGDGTDGSVLSLTVYNSELIAG
                     GSFTTAGGVNANRIARWNGSAWHRWVREQTATSVP"
     gene            complement(9294..11807)
                     /locus_tag="P5316_09620"
     CDS             complement(9294..11807)
                     /locus_tag="P5316_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRR85257.1"
                     /translation="MFERFTDRARKVMALANQEAQRFNHEYIGTEHILLGLVKEGSGV
                     GANVLKNLDVDLRKVRLEVEKLVKSGPDMVTMGKLPQTPRAKKVIEYAIEEARNLNHN
                     YVGTEHLLLGLLREHDGVAAQVLMNLGLKLEDVREEVLNLLGAGIENEESQPHAMGEA
                     KKGKSKTPALDSFGRDLTEMAREGKLDPVIGRHREINRVIQILCRRTKNNPVLLGEAG
                     VGKTAIVEGLAQKIVSGEIPELLADRRIVVLDLAMMVAGTKYRGQFEERIKAVMNEVI
                     RAKNVILFIDELHTLVGAGGAEGAIDASNVLKPALSRGEIQCIGATTLDEYRKYIEKD
                     GALERRFQQIIVEPPTREETIQILQGLRDRYEAHHRVQITDEAIMQSVELSNRYISDR
                     VQPDKAIDVIDEAGACVRLKTMTKPPNLTEIEREIERLLIEKDEAVKTADYERAAELR
                     DRAEVLRSKKDEMQREWRSRAKEVDGIVDAEVIAEVVSSMTGVPLTRLEKGEVERLLR
                     LEDELHKRIISQDEAIRAVARAVRRSRSGMKDPNRPMGSFIFIGPSGVGKTYLAKCLA
                     DFMFGSEDALVVLDMSEFMEKHNVSKLIGAPPGYVGYEEGGQLTERIRRRPYSVVLLD
                     EIEKAHPDVFNMLLQIMEEGRLTDSFGRHVDFKNTILIMTSNIGADRITNQGDFGFGK
                     RDENVTYEKMKKMLQSEVERYFRPEFINRVDELVVFHKLMHDDLTRIVELELAKVVKR
                     IAQQGLKLEIDQSAKEYLIEHGTDEKFGARPLRRAIENRVEDPLSEAILRGEYKGRNR
                     IVVSAKPEGEDGEMKLVFEAMTDEAQSAPVPAGSTGDGT"
     gene            complement(12401..12700)
                     /locus_tag="P5316_09625"
     CDS             complement(12401..12700)
                     /locus_tag="P5316_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85258.1"
                     /translation="MGGILTLVLMNLTVPGSGLILLGRAWLGFALAIWFCLAAVIAVS
                     GRYIAPVSIPWGVTAVAAALALMAWLIGQGLLVSRIRCLRGRLPGALFSVKKTDT"
     gene            12841..13440
                     /locus_tag="P5316_09630"
     CDS             12841..13440
                     /locus_tag="P5316_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85259.1"
                     /translation="MHIQRRIRIGRRRLIAATAIAMFWGGCSSSIDRSPSSVSRGAGA
                     AGRRIANTDVAAVQPVAERVFRQYFRIDSVASGPGLIVAQPQEMTGAAQPERVRDLLA
                     GVRGRHRYLAELRLNQQGPDVLLRCVVQAQRLETSERAAFSSQRGDDRPSDTPIDRMG
                     AGSADNREEWVYGKRDRQLEQEILSAIEEAFRPATSTSE"
     gene            13467..14063
                     /gene="folE"
                     /locus_tag="P5316_09635"
     CDS             13467..14063
                     /gene="folE"
                     /locus_tag="P5316_09635"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRR85260.1"
                     /translation="MPESSKPQQSVDLDRIASAVREILLAVGEDPDREGLKQTPLRVA
                     RMYAEVFSGLHESPRQYLRTFFTEQYDELVVLRDIPFHSMCEHHLLPFAGDAHIAYLP
                     DGRIVGLSKLARVVEAYARRPQVQERMTSQIADLLMEELHAKGVAVVLRATHLCMTCR
                     GVKKPGTLMVTSALRGLCKSDARTRGEVMTLLADSRTR"
     gene            14075..15718
                     /locus_tag="P5316_09640"
     CDS             14075..15718
                     /locus_tag="P5316_09640"
                     /inference="COORDINATES: protein motif:HMM:NF027717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5010 domain-containing protein"
                     /protein_id="HRR85261.1"
                     /translation="MRFEMVLGLLAVLAWRPAAFASAASPLDRPPPGRYFKLRAAVHG
                     KAPTFKSGRPIVGTTYFYWYDVYTGAHLRNPDGSDALTTHPPESFMADLSYKSPDWHY
                     AQLRDMAEANLDFFMPVFWGVPGQYDEWSFAGLPPLVQAHDRMTAGHKEDSTVPRPPQ
                     IGLFYDTSTLRTNVLPDGRRNVRIDLTTLRGREWFYATIRDFFSMIPPQKWARVDGRP
                     IIFLYAASFAEKVDDRLFDDARERFRKDFATDFFLVRHADWPGRSDAWYRWGGALGLT
                     LGDVVAGLGPGYDHSAVPGREALVVPRRGGAFYREQWEKLLRMQPARRPWIVHVETWN
                     EWHEGTDIARSQDWGVRYIEMTARYARLFREGTQVLPEGPYVNADMVRWSLQHTAGLT
                     LRPSAGDGHWKTTTIDGRRCATTVSGPDSLPARFLYFEIDDSFMFDRMDSVAEVSIIL
                     RDDDGCDAIRIEYDSGDRASSVFEGAFRPTRDIPLGKTDTWRMISLHLPDVRFCNRTN
                     GADFRIAAVGGKQELSVAEVMVRRLPGKLVHPESQPVAK"
     gene            15859..16782
                     /locus_tag="P5316_09645"
     CDS             15859..16782
                     /locus_tag="P5316_09645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR85262.1"
                     /translation="MRTINRREFLGLSAAAATGVAGEVFGEKATTTTAPSTGRAASAS
                     DTVVLGKTGIRASRLAIGTGTKGGSIQRNLGSEGMIKLLRHGFDEGVRWWEAADMYKS
                     HPYIGEAMRQVPRDKLVITSKVWAHTPGDVCRPAEDIRADIERFRQELKTDYIDICLL
                     HCLTDPAWRETMKPAMEVLSEAKQKGQVRAMGCSCHSIEALKAASEEPWVEVILARFN
                     PYAMHMDVKKREEIPQVAAILEAAHKRGKAVYGMKIVGEGAFKGDQIDESLRFALSHP
                     FIDAFTIGFANQGELDDMIRRIKQSAAGLGR"
     gene            complement(16842..18251)
                     /locus_tag="P5316_09650"
     CDS             complement(16842..18251)
                     /locus_tag="P5316_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85263.1"
                     /translation="MRRYLLCLLALAMLSGQACNTRPADNGGPSNGGDNTNGGGSDDG
                     PAIGGVPAERLQAADFNYLGAFRLPDEFNWGALGLCYYPDGGGAGSLLVTGFQSPYDS
                     AHPGEACWSEEWNCRAYFGEVTIPTPAVLSNWEDLPVAALRNDMRSFDGGLVSTLNRE
                     WTFVSDIEYVPRQGTQSSDKLYGSLNFWYAEGGVGENTFPTVWFANLDGTQARGLFHV
                     GPDAAPYHGRKMGAYLFTVPGWYADRYLGGRTLVTGRSRGTPRTGFEPVTTDGGSQGP
                     TLFAFRPWDSDNPTGNLDALPVLYYRVAFSDDCLAFPCCGGPNVGNPAQCDYPDFTMC
                     DDWTGAAFVDNGTRRAVMLLGYKGLGNNCYDEPPVDCNDPCSETHGYHCNPYERQVIF
                     YDVHELGTSALGQQNPWVVVPYAIWRPTEFYLAGHTCWNVGGMTFDAAGLRLFMAERG
                     LGEGEMNAVVVHVWSLSAD"
     gene            19095..19616
                     /locus_tag="P5316_09655"
     CDS             19095..19616
                     /locus_tag="P5316_09655"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRR85264.1"
                     /translation="MDVRYINPFILAVQNVFKTMLNVEVLVSKPLIKTKESPTSDVSA
                     IIGLSGPVSGSVTLCFSKQVAVRVASTLAGTELSVYEPADLTDALGELTNMVAGQAKI
                     QLPQSNITISLPRVILGDQHRLLESRTSPVLLLPCDSSIGRFSVEVTLLTKRFCPFTV
                     EVDLGVKRRQQSV"
     gene            19758..>20881
                     /locus_tag="P5316_09660"
     CDS             19758..>20881
                     /locus_tag="P5316_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR85265.1"
                     /translation="MNAMRGHLTNLVRAGIIGTAIVTMVSLSGCAPGRARQCDIMVYG
                     ASAGGTIAAIAAANEGASVILVEPGRYVGGMVSGGLGATDFGNKSVIGGMSREFFVRV
                     GRHYGEPPAWYFEPHVADRVFHDWLKEARVPVVFGQQVEQVEKKGPRIREIRMADGAV
                     YRARVFLDCTYEGDLMARAGVSYTVGREGMDQYGESLAGRREKCEYHQFNTPVSPYDE
                     NGKLLPCVYGGDPGEIGQADHKVQAYNFRICLSNKRENQVPFPKPANYDPYRYELLKR
                     YLQAAPDLTLDKLIHIKMMPNGKTDVNNNGAFSTDHIGGSWDYPDADYKRRQEIWDDH
                     VSYVQGFFYFLANDPSVPKHIQDELNKWGLAKDEFLDTGH"
BASE COUNT         4043 a         6461 c         6372 g         4005 t
ORIGIN      
        1 cggcatggtg agcgagatca cgttgagccg ctgatacagg tcgaggcgga actctccggt
       61 ttgcacaagt atggggagcg ggcggttgca ggcggcaata acccgtacat tgacctggcg
      121 ctcgatatcg tcaccgacgg gcaggatctt gccctcctgg aggacccgca gcagcttggg
      181 ttgcagcggt ttttccaact cactgatttc atcgaggagc agtgttccct tgtcggctgc
      241 caagaagtaa ccggggcggt cttgtgtggc tcccgtgaac gcgcctttcc ggtgaccgaa
      301 aagggcgctt tccgccagtg ttccggttat ggccgcacag ttgacggtca ggaacggata
      361 attgcggcgt ttgggatcca tccgatggat ggcttctgcc aagagttgtt taccggtgcc
      421 cgactctccg cagaccagaa cgggggcatc tgagaccaag gccgcccgct tggcctgaaa
      481 gagcagcttg gccatgatct ggcttctgcc cgcgatgcca gcctcgtcaa agatggctcg
      541 agacctaagg tcttcgccct cgcggacgga ggcctcgtat cggtccaagg cctgtttgag
      601 ggtgtccacc acctggtcca ctgccatgca gggagcccat tccacgaaac tggtggcgcc
      661 tgaaaccacg gcttggcaac aggttgtgac gtcggcaccc tgggcgatca gaactacgat
      721 ttgcgaatga ggcgactgct gtttgatctg cctgaaaacc tggaggccct ggctaagaag
      781 ggtaccggat tgactcgcgt cacaaccgga ctgggccgtc gggccggggc agatggccac
      841 gtttgccgcg tcagcgagat cgtacagcac cagcgggcag aggtcggctg ccaccctggt
      901 cacggcctcc gcagcggact gcacccagac gacatcgaag ccgcctcggg attccagaga
      961 ccgaatcagt ccttcggtac cctctgtgct ggaacggaca agcagaacgg cgttttgaga
     1021 cgtacgacta aaacgcacga tctcctctct cctccctttc ccctctctcc cttcgtcagc
     1081 tacgcacact gccgcgccga caactctccc ttgcccctca ccgctggcgc ggtgacccta
     1141 gcttcatcgt attcagtata ccggcccata tccccacgac gcaaggcccg tggggcggac
     1201 tgctcataaa atggcgaaac ctgtgagtcc tgtgaaactc accgtccgat aggattgccc
     1261 ccgatcttac caagaggtcg agctgctaaa gccttagtga gataatagtt tttcgagtgg
     1321 aaagcggact gccgctactt gagatcatac aggctgttgt ctggcgacag gaaaccgttg
     1381 ttatctccga tgtatggttt ctcaaggtcg taggtctcgg tgtgtctgcg attccatggt
     1441 cgggcgtgac catcggcaaa ggccacattg gtccgcccgg tgtgtcggta tccctgcgtt
     1501 ccggctcctc gaccgctgtt gacccaggtg tcccgagccc cctgctcgga gccccagggg
     1561 gaacgcatga acttgttggc tcccgcgaca tactccccgt ctccaaagag cgcgcacagc
     1621 gagggcattc gaatctcgtc ctgtcgagcg gggcgaacca cgacggacca ggagactgcc
     1681 tgtgccgggg cggccggccc aagagacacg gtcttttcct tcctcttccg gaaagcgcct
     1741 atgtagctgc tgttgtagtt gtatccggtg tagcgatcat ccgcccacat ggccgggccg
     1801 gcaaagcccg gacactgctg aatcgcctca atggtcttgc cctgccagat caagccggga
     1861 agaacacgga ccttgccagt catcaggtcc gtgattcgag tgatgtccca tccgtagtcg
     1921 atctggcggt ctgaatcgcc gttggcggcc ggcgcggtca ccgtgtcatg ggaaggaggg
     1981 taaaagcccg aatacacggc ggtataggcc tgcgtggtcg tgagcatctg atgcagattg
     2041 ctcaggcaaa ccacggtggc agcctgtcgt cgggccctgc tcaacccggg caagagcatg
     2101 gccaggagca aagcgatgat ggcgacaaca acgagcacct caatcagagt gaatgcatcc
     2161 ttgccgatgg ccgaatcaag cagcgacaac cgtcgacgac ggcccttggc ccccgaggtt
     2221 ctgaatccat tcttcatccg tatgctacct cgtgccgtct tggcaccacg tcagttcatg
     2281 cagtcccagt ccagcagtac atccggccct gtgatacagc gttgcaggaa gccgaaatcc
     2341 gtttgatcca catcgccatc ctgatcgaaa tcggcgcgta cacattgttg ggccaagcga
     2401 gccacgccgg ggccgctcgc gcattcctcg aacctgtcga cgtcggcccc gtcgacatca
     2461 gaatcacaat ccaggtccgg caccaaccgg ggagccacgt cggccacggc gtcgatttca
     2521 gggacgcaac cgctgccgat cgggttgtcg atccggacgt agcgaaacca tcgcagcccg
     2581 tgcggtccga caggcaggtt ctgcaaagcg gcgaggtcga agccggtccc gcccgccgag
     2641 tagccgtact tcatcgccag gccggccagc gtccatgcgc tgaacgtcgt cggctcgggc
     2701 accgctccga ggggggcgtg caggtcgggg ctgagcggat acgtcggatc ggtcggctgc
     2761 ccccaccact ggttgccggg gagtgacgaa acggggccgg ccgggtcata gatgcgtccg
     2821 agggttgggg cgaaatcatc agcaaagggc ccatcgacaa acgtgtacca agtcacgccg
     2881 tcctggctga ccgacacagt ggccggttct ttggctattg gcccctggag caaggtctgg
     2941 ttggggtcgc cgttgcgcca cctgctgatg ccgtcgataa tcatgttgga attgccgaag
     3001 atgatgaaat ccacgccgca ggggttgaga ggatcatcca ggacgtggtg gtcgaactcg
     3061 aggatcagat gtccgccctt gccgatcgag acgatctcaa acgcgcgaaa cggctggtag
     3121 accggaacga ccggcacggg gttggcggcg gagcccgtgc ccgaggcgaa atcattgtcg
     3181 ccggtggtgt cgatcgtggg ccggtcgcct gcggcaaacg ggtcattgaa gtcatcgaag
     3241 ctgatgaaat cccaaggcac gccatcacct tcgacgtagt tgcccgggag atcgacatag
     3301 aaggcgaagt cggagcggtt gtactcgtag ccattgacgg ccgtgccggc cgcggtgaca
     3361 accgctccga ggattgcata ccatctgcac atcgcctgtg tctccggctc agccgacgcg
     3421 taccgaacgt ttcagcaaca acgttgttgc cagagcggcg agcagcactg tcacgggctc
     3481 gggtatgggc ataccggcgc ggccgtagat ccatgcgtca gagcacccgt cgatcaccgt
     3541 tcgactggaa gccccgtagg acggggattg ccaattgcct ccgggctcct tgatccagta
     3601 gtgccaccag ttctcgccgc cgccgtatcc cacgttgctg tgaccctcgt agctgatccc
     3661 gtcaacgaac acaccccagc caaaatcgtt ccgaatcgtt gtcaaggttg tgcttgtctc
     3721 gatcacgtcc aagagcccca agcccgttgt ctgaccatcg aaggacacct cgaacacata
     3781 gaaggttcca tccacgaact cgaccgtgac ctcagcgtga ctgttgccgt agccgacagg
     3841 gattggagaa ccctctgcca tggccgtccc cagcagcgac gctcccagga accctgtcag
     3901 gaaaacccgg attgccaccg cccttgtgac attcacatga tccctcatca gacacccttt
     3961 ctgtggccta cctcgggcca caaaaaaatc ccagcgcccg cggacacgag cactgggatg
     4021 atccggcgtt caaacgcaaa cgggattcac gcggccgaca atgaccgcct gaatccccgg
     4081 cagctctcgc aatacccatg cccggggcac acgacacaac aaaccacgtt cgcaggcagg
     4141 tcttctggct tacggagcgg ggaaccgggt catccgaaga agaccgtttc ccacatccgg
     4201 ccacgccttc tcatccgtgc cgcagggcat catgagcccc actttgtcac ggacaatggc
     4261 aagagtagcc ggtactccga tcacagcggc gggtccgcgg tggatttgca ccaccttccc
     4321 ttttcattcc gcccaaggcg gaagccggcg atcgtgcacc tctttacttg tcagggcgga
     4381 atcataaccc gccggcgact gatgtcaagt gccttttgca cccggtttgg cggattcttg
     4441 cggcacgcgg gcggtggtat actgccggcg gtgatgtcga ccgcggtggt ccccaccggt
     4501 aaccagtcaa gcgggcgaac ggtcggtctc cggaccggaa aggacttgcg acaatgagca
     4561 atacaaagca tcatcccgtc accgtcacga cgcgtcgcga ttttctgaag ttttcggcgg
     4621 cggccgccgc tggagccaca accttgagcg tcgccggcgg agcccacgcc gccgggaatc
     4681 aggtcattcg tattggcatg atcggttgcg gcggccggtg cacgggagcc gcggatgaga
     4741 gcatgcaggc cggtccggac gtgaagctca cggccatgtg cgacgttttc aaggataaga
     4801 ttgagaagag ccgcgagagt ctcaagagca agcatcccga gcaggtgcag gtggatgatg
     4861 ctcactgctt tgtcgggctg gacggataca agaaagtcat cgattccgtc gacgtcgtcc
     4921 tgattgcttg tgcgtccaag tttcacccga tgtacgccga ggcggccatc aaggccggca
     4981 agcacgtgtt cgtcgagaag ccccacggca tcgacgcgct tggcgtccac cgcatgaagg
     5041 cggtttgcga gcttgccaag gacaaaaacc tcagcatcct gtccgggctg caaagccgct
     5101 ggcacgccgg ctggcaggag acgatcaaac gcattcatga cggggcgatc ggcgaaattg
     5161 tggccatcca gtgcatgttc cttcgggcac cgtacgtggt cgtcgagcgg gagccgggcc
     5221 tcagcgagct ggagtaccaa ttccgcaact ggtaccactt ctgctggctg tcgggcgacg
     5281 acgtgccgca gtcactggtt cataacgtgg atcgggcgtc gtgggccctc aaagagcagt
     5341 tgccgacctg ggccttcggg ttgggcggcc gttcggcgtc tttcggtgag aaatacggcg
     5401 acatgttcga ccaccacacg gtcgtctacg agtatgcggg tggcgcacgc gtgtacgccc
     5461 tctgtcgaac ccaggagaac acctacaaca actacagcga catcattatg ggtaccaagg
     5521 gcacttgcta tctcggcgaa tgccgcatcg agggtgagaa gaagtgggag ttcgccggcc
     5581 agcacaacaa ccccggcgcg gccgagcaga aggccctgat agaagcagtc cgaaacgaca
     5641 agcccatcaa cagcggctac catatgaaca acagcaccat gatgggcgtc atgggtcaag
     5701 ttgcgtgcta tacgggcaag ccggtcaagt gggaagagat ctggaaatcc gacttcagtt
     5761 tcggcccacc gccggagaag gtcaccatgg acatggaacc gccgaccaaa cccgaagcag
     5821 gcggcgacta tccgattccg attccgggtc agaccagact gctgggctaa acgtcccagg
     5881 ggcttgcgcc cctggctaca tacctgtgcc cctctggggc aacgggcgat ttcgggtttg
     5941 cggccaggct cccaggggct tgcgcccctg gcacatacct atgccccttt ggggcaatct
     6001 gtttttcatg gtcgggttga ggatgacggt tcctcatacc ggcagcatcg cttcgtttgc
     6061 agccggctac tattgtgtgg aggccctggg cctgaaggat gctgacctcg ctaaacacgt
     6121 actcgtgcgg agcacgacct accgggctgt acaggccact cggaaacgct ttggttgcgg
     6181 ccgaaagccg ctttgggaac agggttcgct gcttcgctct gcgagctgcg caaggcaagc
     6241 agggttcagg gcagcctgct tatggcgcac aatccacatc cgccagcaca tccggtccgc
     6301 tcaggcactg caggaagagc gcgacatcgt tcttgtcgac atcgctatcg ccatccatgt
     6361 gtgccggcct gcactcaggt aagccttggg gcacggtgtc gccgctgaga caggcctgaa
     6421 aacgaccgaa atcgtcctgg tccacatcgc cgtcaaggtc gaagtcgccc ggtgggaaca
     6481 tgatggtcaa caaggctgcg ttgccggcga tcgaagcgca gtcgtcggac acaaccacgt
     6541 cgtagctgcc ggcgtcaccg aggccaaccg ggtcaacggt cagcgtcgcg gtcgtcgccc
     6601 cggagatgtt gccgccgtcg gtcaacgcaa cgccatcctt gcgccattga taactcagcg
     6661 tccccgttgt cgcggaagcc aacacattga actgcacggc ttgtcccagc gggctcacct
     6721 gcgaggtcgg ctgctggtca ataacgggca aggaagcctt ccagcgggcc cagtaggccg
     6781 atacttgacc gcccgcggtg gtgaaagagc cgccggcgat cagctcgtca ttgtggacgg
     6841 tcaatgcatg aacagtgtcg ttcaagccgc tgcccattgg ctgccagtcc gtgccgttcc
     6901 accgggcgat ccggttggcg ctcacgccgc cggccgtcgt gaaaccgcca ccggccacca
     6961 gttcgccgtt gtagacggtc agagacaaca cgatgccctc cgtgccgctg cccagtggct
     7021 gccaagctga gccgttccat cgggctatgt tattgcaggt cacgccgccg gctgtcgaga
     7081 ggtgaccccc gacgaccaac tcgccattgt agacagccat ggcccttaca gggcgatagt
     7141 tctcggtcga catcccgctg cctagaggtc gccagacaga gccattccag cgtgcgatgt
     7201 ggttgacgct gacaccgctg gccgaaggaa aaacgcctcc ggcgatcagt tcaccgttgt
     7261 acacagtcag ggcccatacc gtgccgtaga taccgttgcc tagcggttgc cagtgtgtcc
     7321 cgttccagct cgcaacgccg ttggcagtca cgccgccggc ggtagtgaag tagcctccgg
     7381 cgatcaattc gccctgagac agaccgtcag tgtgtggatt tgagggtatg gatcggcggc
     7441 tgatcccgtc attccgctgc ccaatgaccg ccacgacgtg ccgttccagc cgaggacttc
     7501 agcgataaaa tcgttgtcaa cacggtacat cctgctcccg acgatcagct cgttgccgta
     7561 gctggccagg gcatctacat tcggagtctg gccgatcccg cctcccagag gctgccaagc
     7621 tacgccatcc cagcgggcaa cagagtcagc gccaacagca ccaccagtaa tgaagtcgcc
     7681 tccggcaatc agatcgccgt tgtagacggt cagagcaagg actcctccgc tgatgccgcc
     7741 acccaatgac tgccattccg aaccgttcca ccgagcgaca cgagccgtac ctgctccgcc
     7801 ggccgatatg aacccccctc cggcaatcaa cccaccgccg tgcaccgtca gagcacgtac
     7861 cccgggatca ggccaagcaa tcccaagccc gctccccagg ggtttccagg tcgacccgtc
     7921 ccaacgggcg atacacctcg tgtcccagaa actaacgttg cccgcccgat cgaagttgcc
     7981 cccggcgacc agttcaccgt tgtagacggc cagcgcatat accgtcgggt cagtcatcgg
     8041 ggaggcatac attcccaggt ccatctgttg ccatgtggaa ccgttccagc gggctatggc
     8101 gtagacgtgg aagccggcgg cagtggtctt gaacttgccg ccaactatca agtcgccatt
     8161 atgaaccgcc agcgcgtaca catcgctgcc cattccgccg ccgatggacg accagctcga
     8221 tccgttccag cgggccaggt agttcacgta tgaaccgccg gcactgacga aacttccccc
     8281 gatcaccaga tcactaccgt agatggtcag ggcacagacg tcgccgtctg ttcccgtacc
     8341 cagcggtgcc aagccgaacc attccaacgg gcgattcggt tggcgttcac gccgccggcc
     8401 gtcgtgaaac tgccgccggc gatcagttcg ctgttgtaga ccgtcaggga tagcacgctg
     8461 ccgtccgtac catcgcccag cggctgccag gttgagccgt tccagcgggc gatgcagttg
     8521 acggtcgcgc caccggccgt ggcgaactct cccccggcga tcaactcgcc gttccagacg
     8581 gccagggcac gcactgtccc gttcgtcccg tcgcccaacg gctgccaggt tgagccgttc
     8641 caggaggcga tgttgctggc gagattggtc cctgccacgt tgaagctgcc gccgaccacc
     8701 aacagctcat ccagcgggcc ggcgccgtcc ggatcccaac tggtcgctgc gtacaccgtc
     8761 ccattcactc ccggcaaacc ttcgccgggc agccactggc cttggcagtc ttgggcgacc
     8821 gcagaaccgg ccgaggggct caacaacatg acaacgagaa tgacgccgct gaacgatgag
     8881 aacagtttat gcacgtcctg ctcctctccg gcgatgccga atacagaatt ggactccagg
     8941 ccaaacctct cagacggcct acagccgacc gcccactacg ccggctgcct cctcatactt
     9001 gtccatgaca ttgtaaccga agcaacagga gaatcaagtg attaggagaa caaagcaagc
     9061 cagccttcaa gacatgcatg cggcagggtt tgcgccggcg ggccgatttg ggatttgaaa
     9121 tctgcgaatc ccagatctgg ggctgattgg tcggccacag catgcctgcc gctccaatca
     9181 gacgagttct ctcttgggca tctgccaacg agaacggccg ccctcgcgtg gggtcggccg
     9241 ttccctgggt gtttccctgt gccgtccgat cgaatccaca tccagcggtc gggctatgtc
     9301 ccgtcgccag tggagcccgc cgggaccggc gccgactgcg cttcatcggt cattgcttcg
     9361 aagacgagtt tcatttcgcc atcctcgcct tcgggcttgg cgctcacgac aatgcggttc
     9421 cggcctttgt actcgccccg cagaatggcc tcgctcagcg ggtcctcgac gcggttctcg
     9481 atcgccctgc gcagcgggcg ggcaccgaac ttctcatcgg tcccgtgctc gatcaggtat
     9541 tccttggctg actggtcgat ctcgagtttg aggccctgct gggcgatgcg cttgacgacc
     9601 ttggcaagtt cgagctcgac gatgcgggta aggtcgtcgt gcatgagctt atggaagacc
     9661 acgagttcat cgacgcggtt gatgaactcg ggtctgaagt agcgttcgac ctcggactgc
     9721 aacatctttt tcatcttctc ataggtgacg ttttcgtcgc gcttgccgaa gccgaaatcg
     9781 ccctggttgg tgattcgatc ggcgccgata ttgctggtca tgattaggat agtattcttg
     9841 aagtctacgt ggcggccgaa agaatcggtc agccggcctt cctccatgat ctgcaggagc
     9901 atgttgaaca cgtcggggtg ggccttctcg atttcatcga gcagtacgac cgaatacggc
     9961 cggcggcgga tgcgttcggt gagctggccg ccttcttcgt agcccacgta gcccggcggg
    10021 gcgccgatga gcttgctgac gttgtgtttc tccatgaact ccgacatgtc gaggaccacc
    10081 aacgcgtcct cgctgccgaa catgaagtcg gcgaggcact tggcgagata tgtcttgccg
    10141 acgcccgacg gaccgataaa gatgaagctg cccatcgggc gattcgggtc tttcatgccg
    10201 ctgcgtgaac ggcgcacggc ccgggcgacg gcccggatgg cctcgtcctg gctgatgatc
    10261 cgcttatgca gttcgtcctc cagccgcagc agccgctcga cctcgccctt ttccaggcgg
    10321 gtcagaggca cgccggtcat gctcgagacc acctcggcga tgacttcggc atcgacgatg
    10381 ccgtcgacct ccttggcccg gctgcgccac tcgcgttgca tttcgtcttt tttggatcgc
    10441 aggacttcgg cgcgatcccg cagttcggcg gcccgctcgt agtcggcggt tttgaccgct
    10501 tcgtcctttt cgatcaagag cctttcgatt tcgcgctcga tctcggtcag attcggcggt
    10561 ttggtcatcg ttttgaggcg tacacaggcg ccggcctcgt caatgacgtc gatcgccttg
    10621 tcaggctgaa cgcgatcgga gatgtatcga ttggacagct cgaccgattg catgatcgcc
    10681 tcgtcggtga tctgcacgcg gtggtgagcc tcgtagcggt cgcgcaaacc ctgcaggatc
    10741 tggatggttt cctcgcgggt cgggggttcg acgatgatct gctggaatcg ccgctccaga
    10801 gctccgtcct tttcgatgta cttgcgatat tcgtcaagcg tggtggcgcc gatgcactgt
    10861 atctcgccgc ggctgagagc cggcttgagc acgttggagg catcaatggc gccctcggcc
    10921 ccgcccgcac ccaccagggt gtggagttcg tcgatgaaca ggatcacgtt cttggcgcgg
    10981 atcacctcgt tcatgaccgc cttgatacgt tcttcaaact gcccgcgata cttggtcccc
    11041 gcgaccatca tcgccaagtc aagcacgacg atccggcgat ccgccaggag ttcgggaatc
    11101 tcgccgctga cgattttctg ggccagtccc tcgacgattg ccgtcttgcc cacgccggcc
    11161 tcgccaagga ggaccgggtt gttcttcgtg cgacggcaga gaatctgaat cacgcggttg
    11221 atctcgcggt gtcgaccgat cacggggtcc agtttgcctt cgcgggccat ttccgtcagg
    11281 tcgcgcccga atgaatcgag ggccggcgtt ttgctcttac cttttttggc ttcgcccatc
    11341 gcgtggggtt ggctctcctc gttctcgatg ccggcgccaa ggaggttcag aacctcctcg
    11401 cggacgtctt ccagcttgag accgagattc atgagcacct gtgccgccac gccgtcgtgt
    11461 tcgcggagca ggccaagcaa gaggtgttcg gtccccacgt agttatgatt gaggtttcga
    11521 gcttcctcga tcgcatactc gatgaccttc ttggcgcgcg gcgtctgggg aagcttgccc
    11581 atggtgacca tatccgggcc gctcttaacg agcttttcga cttccaggcg caccttccgc
    11641 aggtcgacat cgagattctt caggacattg gcaccgacac cggagccttc tttgacaaga
    11701 ccgaggagga tgtgctcggt tccgatgtac tcgtggttga agcgctgggc ctcctggttg
    11761 gccaaggcca tgactttgcg agcccgatcc gtgaaacgtt caaacatccc tagctcctgg
    11821 cagcgcggtg tttgtgaatc agcccgctgc tcctgacggc gtcccgctca ccggttccac
    11881 ccgacacggt tgcatttacg ctgtaagccg tatcttcgag cgggaacaca gcttgtctgc
    11941 ccctaccggg cgcctgcaac agactaggcc atcgctaacg gagatcgcac ccccggggcg
    12001 gttgttctgg gacgcacggg attctagcac tgccggcgag ctccttcaag ctaccgcaca
    12061 tatcctcgca aacccgtcag cttccatatc ggtcagcttc caccgccagt tgtgggtttt
    12121 cgcctagggc ccccgggccg atctttgacg ccctcgtgaa cctgctagtg gtcctgtggg
    12181 aggcggtcca agcggtgtcc ccaggaagga ttggggcaac tcggatagtg caccgctttt
    12241 ccccaggcgt cgccgaccgc caacgcggcc cagggtggcg cccgagtgca caacctggtg
    12301 cagaatcaag agttattatc ggcaccccgg ccggctcccg gcatcacctg caggagttgt
    12361 tcacaattgt ttctggtctc cgttggcacc cgctagcatc tcatgtgtcg gttttcttca
    12421 ccgaaaacag cgcgcctggc aggcggcccc gaagacaccg gattcgggag accagtagcc
    12481 cttgtccgat cagccacgcc atcaacgcga gagcggccgc gacggcggtg accccccagg
    12541 ggatgctgac cggggcgatg tatcgtccgg agacggcgat gaccgcagcc aagcagaacc
    12601 atatggccag cgcaaagccc agccatgccc ttcccagaag aatcaggccg ctgccaggca
    12661 ccgtgagatt catcaaaacc aaggtcagga ttccgcccat gccgcacgat tatagctcgc
    12721 cggcgcagac ccgccacgac atcattttct tgaactaccc tgcgaaccat cgcataatgc
    12781 acctcgtgct gatcgttgtg aatccattga ggcgccagag tctcccgcgg agttcagccc
    12841 atgcacattc aaagaaggat ccggattgga cggcggcggt tgatcgccgc gaccgccatc
    12901 gccatgtttt ggggcggctg ctccagttcg attgaccgtt ctccatccag cgtcagtcgc
    12961 ggtgccggcg cggcaggaag acgaattgcc aacaccgacg tcgccgccgt gcagccggtt
    13021 gccgaacgcg tattccggca gtacttcagg attgactccg ttgcatcggg tccgggtttg
    13081 atcgtcgctc agccgcagga aatgacggga gccgcacagc ccgaacgcgt gcgtgacctc
    13141 ctcgccggcg tcagagggcg tcaccgctat ctcgccgagc tgcgactgaa ccagcaaggc
    13201 cccgatgtcc tgctgcggtg tgtggttcaa gcccaacgtc tcgaaacctc cgaacgtgcg
    13261 gcgttcagtt cgcagcgtgg cgatgatcgt cccagtgaca ccccaattga ccgcatgggt
    13321 gcggggtcgg cggacaaccg tgaggaatgg gtatacggca agcgcgaccg ccagctcgag
    13381 caagagattc tgtcggcgat tgaggaagct tttcggccgg ccacctcgac ctcggagtga
    13441 agacgacttg aaacggggaa gatacggtgc ctgaatcgag taagccgcag cagtcggtag
    13501 atcttgatcg cattgcgtca gccgtgcggg agatcctgct ggcggtcgga gaggaccctg
    13561 accgcgaggg cctcaagcag acgcctctgc gtgtcgcgcg gatgtatgcg gaggttttca
    13621 gcggcctgca tgagagccct cgccagtacc tgaggacctt ttttaccgaa cagtacgacg
    13681 agctggtggt gctgcgcgac attccgttcc actccatgtg cgagcaccat ctgctgccgt
    13741 tcgccggcga cgcccacatc gcctacctgc cggacggccg gatcgtgggg ctctcgaagc
    13801 tcgctcgcgt ggttgaagcc tatgcccgcc ggccacaggt gcaggagcgc atgacaagcc
    13861 agattgccga ccttctgatg gaagagctgc acgccaaggg ggtggcggtg gttctcaggg
    13921 ccactcacct gtgcatgacc tgccgcggcg tgaagaaacc cgggacgctg atggtcacgt
    13981 cggcgctgag ggggctgtgc aagtccgatg ctcgcacccg cggcgaggtg atgacgttac
    14041 tggccgattc aagaaccagg tagggagagt cccgatgcgt tttgagatgg tccttggtct
    14101 tcttgccgtt ctagcctggc ggccggccgc tttcgcgtca gcggcctctc cgctcgatcg
    14161 tccgccaccc ggcaggtatt tcaagctaag agccgccgtg cacggtaaag caccgacgtt
    14221 caagagcggc cgccccatcg tggggacgac ctacttctac tggtatgacg tgtacacggg
    14281 agctcacctc cgcaatcccg acggctcgga cgctctcacg acgcatccgc ccgaatcgtt
    14341 tatggccgat ctgagctaca agtcgcccga ctggcactac gctcagcttc gcgacatggc
    14401 cgaggccaac ctggactttt tcatgccggt gttctggggt gttcccggcc agtacgacga
    14461 atggagcttc gccggcctgc ctccgcttgt tcaggcccac gatcggatga cggccggaca
    14521 taaggaagac tcgactgttc cccgtccgcc gcaaatcggc cttttctacg acacgagcac
    14581 gctgcggacg aacgttctcc cggacggccg gcgcaacgtc cgcatcgatc tgacgaccct
    14641 gcgcggccgt gagtggtttt acgccacgat ccgcgatttc ttcagcatga tcccgccgca
    14701 gaaatgggcc cgcgtcgacg ggcggccgat catcttcctc tacgccgcca gctttgccga
    14761 gaaggtcgac gatcgcctgt tcgacgacgc ccgcgagcgg ttccgaaaag atttcgcaac
    14821 ggacttcttc ctcgtccgcc acgccgattg gcccggacgg tctgatgcct ggtatcgttg
    14881 gggcggagct ctgggcctga cactgggcga cgtcgtcgcg gggctgggtc cgggttacga
    14941 ccattcggcg gtccccggtc gtgaggctct cgtcgttccc cgtcgcggcg gggcttttta
    15001 tcgcgagcaa tgggagaagc tgctccgtat gcagccggca cgacgtccct ggatcgttca
    15061 cgttgaaacg tggaacgaat ggcacgaggg cacggatatt gcgcgctcgc aggattgggg
    15121 cgtgcgttac attgagatga ctgcgcgtta tgcccgcctg ttccgcgagg gcacgcaggt
    15181 gcttccggag ggcccttatg tcaatgctga tatggtcaga tggtctctgc aacacacggc
    15241 cggtctgacc cttcggccca gcgccgggga cggacactgg aaaaccacga cgatcgacgg
    15301 ccggcgctgc gcgaccaccg tctccgggcc ggattcgctg ccggcgcgtt ttctttactt
    15361 cgagattgac gattccttca tgttcgaccg gatggactcg gtcgccgagg tgagtatcat
    15421 ccttcgcgat gatgacggct gcgatgccat ccgcatcgag tatgacagcg gcgaccgggc
    15481 ctccagcgtc ttcgagggtg cgttccggcc tacgcgcgac attccactcg gcaagaccga
    15541 cacctggcgg atgatttccc tgcatttgcc cgatgtgcgg ttctgtaacc ggaccaacgg
    15601 cgccgacttc cgcattgcgg ccgtcggggg caaacaggag ctcagcgtgg cggaggtcat
    15661 ggttcgccga ctgcctggca agctcgtgca tcctgaatcg cagcctgtcg ccaagtgaca
    15721 caagaaaaac ctttgactcg gccgcgacaa acaggtagga tgcgaattga ctgctgatac
    15781 cacgtcgtcg cttcgtgagc aatcggcgag aattgccatt ggggagactg cagcgcggca
    15841 acctgcggag atgggggcat gagaacgatc aaccggcgag agtttctggg tctgagtgcg
    15901 gcagccgcga ccggagtcgc aggggaggtg ttcggggaaa aagccacgac gaccacagcg
    15961 ccttcgaccg ggagggcggc gagcgccagc gacaccgttg tgctcggcaa gacgggcatc
    16021 agggcatcgc gcctcgcgat cgggacaggc accaagggcg gaagtatcca gcgtaacctt
    16081 ggcagcgaag gcatgatcaa gctgcttcgg cacggctttg atgaaggtgt gcgttggtgg
    16141 gaagcggcgg acatgtacaa gtcgcacccc tacatcggcg aagcgatgag acaggttcct
    16201 cgcgacaagc tcgtcatcac atcgaaggtc tgggcgcata cgccgggcga cgtctgccgc
    16261 ccggccgagg atatccgcgc ggacatcgag cgttttcgcc aggaactcaa gaccgactac
    16321 attgatatct gtctgctgca ttgcctgacg gacccggcgt ggcgggaaac aatgaagccg
    16381 gccatggagg ttctcagcga agccaaacag aaaggtcagg ttcgggcgat gggctgttca
    16441 tgccactcga ttgaggcgct caaggccgct tccgaagagc cctgggtcga ggtgattctg
    16501 gcccgcttca acccgtacgc catgcacatg gacgtgaaaa agcgggagga gatcccgcag
    16561 gtagccgcga tcctggaggc ggcccacaag cgaggcaagg ccgtctatgg aatgaagatc
    16621 gtcggcgagg gggcgttcaa aggcgaccag atcgacgagt cgctgcgctt tgccctgagc
    16681 cacccgttca tagatgcgtt caccatcggc tttgccaacc aaggcgaact ggacgacatg
    16741 atcaggcgga tcaagcagag tgcggcaggt ctcggtcgct gaagcgccct cgacgccgcc
    16801 ctaccgaccg gttcaagtgt ttctcattca cgcaggcgtg gtcaatcggc tgacagcgac
    16861 cagacgtgaa ccaccacggc gttcatttcg ccctcgccaa gaccccgctc ggccatgaac
    16921 agtcgcagcc ccgcagcatc gaaggtcatg ccgccgacgt tccagcaggt atggccggca
    16981 agatagaact cggtcggccg ccagatcgca tagggtacca cgacccaggg gttctgctgc
    17041 ccgagggcgc tggttcccaa ctcgtgaacg tcgtagaaga tgacctgccg ctcgtaggga
    17101 ttgcagtggt agccgtgggt ctcgctgcac gggtcgttgc agtcaaccgg cggctcgtcg
    17161 tagcagttgt tgcccagccc cttgtaacca aggagcatga ccgcccgtcg agtgccgttg
    17221 tcgacaaacg ccgcgccggt ccagtcgtcg cacattgtga aatcgggata gtcgcactgg
    17281 gcggggttgc ccacgttcgg tcctccgcaa cacggaaacg ccaggcagtc atccgaaaac
    17341 gccacgcggt aatagagaac cggcaaggca tcaaggttac ccgtcgggtt atcgctgtcc
    17401 cagggccgga aggcaaacag cgtcgggccc tgggaaccgc catcggtggt gaccggctcg
    17461 aacccggttc gcggggtacc gcgcgatcgg ccggtgacca gggtgcggcc gccaagatat
    17521 cgatccgcat accagccggg caccgtgaac aggtaggccc ccattttgcg gccatggtac
    17581 ggtgccgcat ccggaccaac gtggaacagg ccacgggcct gtgttccgtc gaggttggcg
    17641 aaccagacgg taggaaatgt gttttcgccg actccgcctt cggcgtacca gaagttgagc
    17701 gagccataca gtttgtcact gctttgcgtg ccttgccgcg gcacatactc gatgtcactg
    17761 acgaaggtcc attcgcgatt gagggtcgag accaacccgc cgtcgaagct tcgcatgtcg
    17821 ttgcggaggg cggccacggg caagtcttcc cagttggata ggacggcagg tgttgggatc
    17881 gtcacctcgc cgaaatatgc ccggcaattc cactcctcac tccagcaggc ctcgcccgga
    17941 tgcgctgagt cgtatggcga ctgaaacccg gtcacgagca gcgagccggc cccgccgccg
    18001 tcggggtaat agcaaagccc caaagctccc cagttgaatt cgtcggggag ccggaacgcg
    18061 ccaaggtagt taaaatcggc ggcttgcagc cgctccgccg gtacgccacc gatggcgggc
    18121 ccgtcatcag atccgccgcc gtttgtgtta tctcctccat tcgagggccc gccgttgtca
    18181 gccggccggg tgttgcacgc ctggccggac aacatcgcca aggccaggag acacagcaga
    18241 tatcttcgca tgttgttgtc ctccaaggag tagaaatgct cgggctccct gtggcgtggc
    18301 ggtcacggca accaggtaca ccttgggcct ccgatgagag cattttgcca caaaacgtta
    18361 cctcggcaca gagccaggcg aatcacattg taaagaactg cattttccgg acggtcgaaa
    18421 ggcagtgtac tctgatccgg ttcgcgttgt caagcttata ccgccgcagt ctcccgccca
    18481 tctgagacgg gcgcaggaac aaaccgctga agcgattcgg ctgcacttcg aaaggaagtc
    18541 cagtgtgagc gtcgtgcgcg tttaccagtg cggtatgggc attgctgcgt gattgtcttg
    18601 accattctgg tgtgaagata ccggccgccc cgttgcaggc tttgagagag ccgagatgta
    18661 ttaccgaaac agcccctgcg agcttccggc ccgtcgtatg gcgaagggcc tgcaagccat
    18721 tgcgagctca gcaagaagcc cggctttgcc aaacgatatc tgaaaaccat acagccgctc
    18781 tcgattgaag ggcggggcca aggcgaaata gtcgagcgca aggaggcata agcccagccg
    18841 gtgtcggccg accacgtttg ccggagttcg cccgtcggtc ggattggtat gttcactggg
    18901 cgagcactcc gccgaggcag tgatactctg gcgccacgcg aatcaggtag cagacttcag
    18961 ggaaaacccg agttggcgca aacaggcgga ccgatacaat ggcaatctgg cgctggttat
    19021 gctgtcgaac gaaagggcgc cggacgcccg acacaatcag gagacgcaga ggcgtcgaaa
    19081 ggatcggtag gtgaatggac gtacgctaca tcaacccgtt cattctggca gtccagaacg
    19141 tattcaagac gatgcttaat gtggaagtac ttgtcagcaa gccgctcatc aaaacgaagg
    19201 agagtccgac ttccgacgta tcggcgatca tcggcttatc cgggccggtg agcggcagcg
    19261 tgaccctgtg cttctcaaag caggttgccg ttcgggtggc cagtacgctg gccggcacgg
    19321 agctttcggt ttatgagccg gcggatctca ccgacgccct tggcgaattg actaacatgg
    19381 tggccggcca agccaaaata cagctcccgc agtcgaatat cacgatctcg ctgcctcgcg
    19441 ttattctcgg cgaccagcac cgcctgctcg aatcccgcac ctcgccggtc ctgctcctgc
    19501 cttgtgattc gtcgatcggt cgcttctcgg tcgaagtaac cttactgaca aagaggttct
    19561 gcccgttcac ggtcgaggtc gatttgggag taaaacgcag acagcaatcg gtttgaggag
    19621 cctctcgggg cagtcagtag ctcgaggttc tgccgaatac gcagtcgggc cggggtttgc
    19681 ccgaaaccag cgcgcccacg cagggagatt gtcccggccg tctattcggg cagcacacat
    19741 acttcggaga cgacacaatg aacgcgatgc gggggcatct gacgaatctg gtacgagcgg
    19801 gcatcatcgg gacggccatc gtgacaatgg tttcgctgtc aggctgtgca cccggacggg
    19861 cacggcagtg cgacataatg gtttacggtg cgtcggcagg cgggaccata gcggcgatcg
    19921 cggcggccaa cgagggagca tcggtcatcc tggtggagcc gggccggtat gtcggaggca
    19981 tggtctccgg cggcctgggg gctacggatt tcggcaacaa gagcgtcatt ggggggatgt
    20041 cgcgggagtt cttcgttcgc gtcgggcggc actatggcga gcccccggcc tggtatttcg
    20101 agccgcacgt ggccgacagg gtattccacg actggctcaa ggaggctcgc gtcccggtgg
    20161 tgttcggcca acaggtggag caggtggaga agaaaggtcc gcgcatccgg gagattcgca
    20221 tggccgacgg ggcggtgtac cgcgcccgtg tgttcctgga ctgcacgtac gagggcgacc
    20281 tgatggcgcg ggcgggcgtg tcttacacgg tcggccgcga ggggatggac cagtatggtg
    20341 aatcgctggc cgggcggcgc gagaagtgcg aataccacca gttcaatacg cccgtgtcac
    20401 cttacgacga gaacggcaag ctgctgccct gcgtttacgg cggcgatccg ggcgagatcg
    20461 gccaggcgga tcacaaggtg caggcctaca atttccggat ctgcctgtcg aacaagagag
    20521 aaaaccaagt gcccttccca aagccggcaa actatgatcc ttatcgctat gaactgctga
    20581 agaggtacct tcaggccgcg ccggacctga cgctggacaa gctcatccac atcaagatga
    20641 tgcccaacgg caagaccgac gtaaacaaca acggggcatt ctcaaccgac cacatcggag
    20701 ggagctggga ctaccccgat gccgactaca agcgacgaca ggaaatctgg gacgaccacg
    20761 tgagctacgt gcaggggttt ttctacttcc tggcgaacga cccgagcgtt ccgaagcaca
    20821 tccaggatga actgaacaag tgggggctgg ccaaggacga gtttctggat accggccact
    20881 g
//
LOCUS       DAOTPW010000087        20683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2644_length_20683_cov_5.243310, whole genome shotgun sequence.
ACCESSION   DAOTPW010000087 DAOTPW010000000
VERSION     DAOTPW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20683
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2644_length_20683_cov_5.243310"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2402
                     /locus_tag="P5316_09665"
     CDS             <1..2402
                     /locus_tag="P5316_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRR85266.1"
                     /translation="MYRRLVRQSNIAAAEGDYTAALDAAMRLRRSWLPAAMIERILDP
                     GLLDALVGARLNQLGRCQEALEWCDRGLRIARNPRTKAILHECAAMVHSTIGDATRCE
                     QHLTALAEVLQKHRIPEIEWGATAMFCANNLGQLEEAWQIARQESDRRGGQVSNRWVA
                     AAIQALENLGRHAEVLHLTEWVLQQGTQRTRSIPAAHNPEAAVGAAELRASYLRTIHG
                     LSLLGGAESTLESGQYDLCERYLAVLESIDLKEPVLRALLTGLRSKLSAHTGDRAAVV
                     DAARTIDELTAKYPEHRRLRPVANLALAESWQILGEHERAISLLSQIDPAVLSLVDRS
                     LLAALMAKSLETLGQTAQAQAKREEAQRLAPAAYWNRPSEAADEAAVETAAPGITLPG
                     ETLIIRSAVEPLADQPVEPVASGTACAAWILAVPALIPLVGSLPAIALFILSLILLFR
                     DKRRLHDRRVGVAGLAISVFSLGCAGLAGFNVAKMALDHIRSKHAAAKTEYSDAEQEE
                     SQDAPPVTSFAPEHEQDETDDIPVLEETEEPAPMPASTLPEEGIRKQPQPIWHHVVVL
                     AVLVVSIIFHEIAHGVAAWWSGDPTARDLGRLNLNPIRHIDPFGSIIVPLVLSLLPGD
                     TVIGWAKPVPVRPQKYRNFRRGNLGVSLAGVSLNLMLALFAANILMVLIILLGVVHPA
                     ERYFDPVALLLGRLHLPGVPYASLWVGGIKICTTAVLINLALANLNLLPLPPLDGFGA
                     LHSFLPNFASALANKLGGVGLIVLFFLLASRIGYILFAPTIVVGALLLLLVMFLGGWA
                     I"
     gene            complement(2434..>3239)
                     /locus_tag="P5316_09670"
     CDS             complement(2434..>3239)
                     /locus_tag="P5316_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85267.1"
                     /translation="RVDLAHRWLSYAVGVVMGRFRPGVQGALGCGRFDARTASGLRRL
                     VVSDGIAELSGDGGDDVAELVMRVLRMTLGDRGTEEVVGYATGKHRDLIVGLRRYLAG
                     RFFRDHLRRYHGRPVYWLVQSCGRGYSAYLYYERLSVTALERLRGPQHVAGRLSEAIG
                     RGDSSLAADLQALNEAIERILSASDEEGRTVGWQLEPDDGVLINAAPLSELLSPVWKE
                     PAECWRRLSAGEYDWSFTAARYWPQRVRGKCGEAKSLDLAHSAARARAG"
     assembly_gap    3240..3339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3340..5800)
                     /locus_tag="P5316_09675"
     CDS             complement(<3340..5800)
                     /locus_tag="P5316_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRR85268.1"
                     /translation="MLASSVPSPARVGESSDTKSALDAILDEPLLAEAWQPGNEESLG
                     WFYQYFNEPDIAVFRGSRPPKVTAELLPAKTQLFTPRWIVRFLVENTLGRLWLQMHPD
                     SRLADRLEYLVPPAEPLPHAKLKPACEIRVLDPACGAMHFGLVVFDLLAEMYREELEH
                     TGQPGWPDRPSVAGKSAIPAAILANNLFGFDIDPLAVRLSAVTLYLKAVSLTPNTPIV
                     PLHLYCADALRLAGGMLWEHNREIGEATTDVFEATYDVVVTNPPYLDSRDYDASLKSF
                     IANAYPAGKRNLYGAFLERCVGLLAEGARLGIITPQTFMFISSFADLRDVLRRSVAIE
                     TLVQGGLGTFPDAVVDAAFYVLRREADPGNRDDCLGTFFRLTRASGAQAKQQAFEQAV
                     TVLRRGEADSRVFRCRQGDFDHLPGRPWVFWITPRVRGLFDHLPRLREIARPTVGLQT
                     SDNFRFLRFWWEVFFRDDPDDAVAANRCIAFGCRDRAAALATGRKWFPHIKGGDFRRW
                     YGNQEYVVNWANDGAEIRTLGIESGKVASRPQNMDYYFRSGVTWTHTSSRGLSVRILP
                     PGFICNVEGMAAFQLDGRGSSLETLAIMNSSFARHVIDLLNPTVHFGGREVGDLPYPQ
                     ADPSAVEALESHVSRAIELARRNSLEDETTWEFVQPPDWRTGPEAAKRRRQELAEIER
                     LIDEEVYTLYVLGPEDRAAIEADVNGDPPQPPLAKGGRTASRLPRGGGERGGSLCRDS
                     HLDTGDARFPPRNEVGGKDSPDEVCENGFSHERDEGCEKGFSHERDEGCENGFSHQRR
                     VDLAHQRRVDLAHQRRVDLAHQ"
     gene            complement(6376..6894)
                     /locus_tag="P5316_09680"
     CDS             complement(6376..6894)
                     /locus_tag="P5316_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85269.1"
                     /translation="MATPEEPDQQPLSPISRRAFFRTLGSHLIKLGAIGTALGSLGQG
                     CWIDIEPDDDHASGGWLYDHYLDFGYNDYSDYGNYSDWGGDDACSYCDGAGQDYSHCD
                     GSYCDYGNYADYYDYSDYYDYANYSDYSNYGNYSDYSNYGNYSDYANYYDYGDYYDYM
                     DWGYCDGWYCDY"
     gene            7148..9124
                     /locus_tag="P5316_09685"
     CDS             7148..9124
                     /locus_tag="P5316_09685"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HRR85270.1"
                     /translation="MNLLCAGTLSKGSLRVQTCPRHFARALGIVLASLLAGSSAASAQ
                     PTESRLGLHMIINYTSGASQVVNAHPRIHKILDTHGAMLASARDFKNNNPNGVLVLRI
                     YTTKSYNISHDPAWAATDFWNTVLAPPINSLSAQDRALIDYVEGPNECDSTPCWGSIQ
                     NAQWLNNFWLSLAPLIANAGFRPCAFCIPVGNPPGSLSDMQAQLDAVVPALRVCKSYN
                     GGWSYHAYTLPYSKSVSTEIWYSLRYRQFYSYFRSTYPDLADLPLLLTEGGVDGQSGE
                     GGPGWKVDDAARYVDWLTWFDARMKEDPYVVGCTLFQSGDLSGWFSFDTEEINPWLAQ
                     HILSSVPPTPPAVPANLNAAINGPTSVQLTWSSASGAISYNVKRSMTGGDSYSTIGTS
                     TTLSYTDNDLTLGATYHYVVSAVNNVGESANSNESTITVTGGYAANSGGSSAGRFAAD
                     TCYDGGYTYMTSASVDTTGATDPAPQAVYQSERWASPSFTYTFPNLVAGAEYTVRLHF
                     AEIYFTSAGKRRFNVSINGAQVLTNFDIIGTAGAANKAVVHTFTTNANPSGQVIIQYS
                     AGSADNPKAGGIEIIRNLPPVYMGDFDQDLDVDQRDFGLLQACLSGNGITQNDPSCTA
                     ADLDGDADVDLADFGVFLNCLSGERVMPPESCRT"
     assembly_gap    9352..9451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9583..10902)
                     /locus_tag="P5316_09690"
     CDS             complement(9583..10902)
                     /locus_tag="P5316_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85271.1"
                     /translation="MTNKTSRRRFLKTTIAAGAGFWVSGRPARAAGKSPNEKLGVACV
                     GIGGRGADNVGAMMNERIVALCEVDEVRGGQTFKRLSNVTKFADYRVMFDKLHKQIDA
                     VVVSTPDHMHAPITLAAIELGKHVYCEKPLVRTVAEARRVAEATAKHKVVTQMGNGGN
                     AQSGARRNVELLRAGVLGTVREIHSWSDRPGTFWKQALDRPIETPPVPATLKWDLWLG
                     VAPQRPYHPAYLPGKWRGWYDFGTGAIGDMACHICNVAFWGLDLRDPTAIECECSEPY
                     EETYPAWSKVKWEFPGNSDRPGVRLYWYDGGKKPSPELIENRELPDNGMIVVGEKGTL
                     FMPSADGAARVLLPEDKFKDLKQPPRTIPDSPGHHEEWIRNCKNGDLGLDYMSHFGRA
                     SVMTESLLLGTVAVRVGKRIEWDAKNRKITNIPEANQYIDPPYRKGW"
     gene            11025..11987
                     /locus_tag="P5316_09695"
     CDS             11025..11987
                     /locus_tag="P5316_09695"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRR85272.1"
                     /translation="MLWQRIAFGTLMIAVVVGLAWLDGWLSACGLTSLDEGVLGGATR
                     IRLMCGLALTLALALLIMLAVFEMGRLSRQGGHNPATNWAAFVSVLLMLAPWIEAQQK
                     LSSAVPALRLTGGMPLSLFLLTGGILGASLCILARKTTERAFSNLAVTCLMMLYLGLL
                     GSFVIRIRCLDAGPAGAALVIFFILTVKAADIGAYFTGIACGKHKLAPWVSPGKTIEG
                     AAGAVVLAAFAACAAIWLWECCLDSTLGPPPFSLAQAVIFGLVTAVCGHLGDLVESLM
                     KRDLGSKDSGQVVPAFGGLLDILDSPVFTAPIAWWLLTFFGRIG"
     gene            12078..13019
                     /locus_tag="P5316_09700"
     CDS             12078..13019
                     /locus_tag="P5316_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRR85273.1"
                     /translation="MELTITLNDARKRNALFGSADRHLRQIRGAFGIQLSARDSTVKL
                     SGAAHAVGQAASVIDFLQRAVRDSDELTDEQVSEAIGYVIQLNNPENRDEDIIEVYSR
                     TTVTRPRTDGQRAYVQAMRGHDLVFCVGPAGSGKTYLAVAVAVSMLKAGTVKRIVLVR
                     PAVEAGEKLGFLPGDMQAKVNPYLRPLFDAMGDMMTFEQIRRFMENDVIEIAPLAFMR
                     GRTLNNAVVILDEAQNTTRLQMLMFLTRLGHHSKMIVTGDDSQIDLLDPESSGISDAL
                     RRLRCIPGIAIIRLEKQDIVRHRLVQDIVNAYANGKP"
     gene            13241..15586
                     /locus_tag="P5316_09705"
     CDS             13241..15586
                     /locus_tag="P5316_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRR85274.1"
                     /translation="MSLRRRVRPSSLLMGVVFYIGVLVVQFWGSDTMPWSLQQLVDRD
                     INARVDFFYEDQAATEANRQKARDGAPSVFVFDAGTAQVEPQTVIRTQMHELRELVAK
                     AENSDAKAIRDQAAAKGWMLDDGAVSALRAFAPAARKAEYERMVDALLSRLLIVNTPE
                     DPNRKSTPPKVLLRTKDKNGATTDKEVGSDLLIYLPKAKDTKIDDQIMAAMHGVRSCP
                     EALHKPFADIVLKVVAGPAKDKPSYTPFWQYNAALTAEQMNAAAELVKEEIVSYKAGT
                     TLVGAKTVLGPAELNLLKHEHKAYLEAQKTDPVLRQRRLLRQLGTAVVVLIVVTGFAA
                     YVVSYRPRITEVPSRTLALTGLLLTMIVLSHLVDRLQGFGSDFPAETCVFFVVIASAL
                     LTIAYDQRFGFGVAAVLTVLTTLTVDGDFNLFLNYMTAAGVTVALLKDIRTRSKIIAV
                     GAITAVATALVSAATSLVAEQPWLYIVTRAALSGSMALFAGFIVQGILPHFEKVFGVA
                     TSMTLLEWCDASKPLLRRLAQETPGTYSHSLVLSQMAEEACETIGAHGLLARVGALYH
                     DIGKAQKPDYFVENQEARMNRHDRLSPTLSLLIIVGHVKDGIEMARAYGLPRVLHQFI
                     NEHHGTTVVRYFHHMASEAAAKSPLKGKHDRQVSESEFRYPGPKPRSKESAILMLCDA
                     CEGAVRALNEPTPGRIESTVHHVVMDRLNDGQFDDCEINLRELSLVEQSIVRSLCAIH
                     HGRIKYPKADRPAPPRAPVPENEAGAMRNGAADVPEVVEQA"
     gene            15583..16122
                     /gene="ybeY"
                     /locus_tag="P5316_09710"
     CDS             15583..16122
                     /gene="ybeY"
                     /locus_tag="P5316_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRR85275.1"
                     /translation="MRTKTHATQTGSADCRITARRFATCHEVNTSRIVQAVRAALGNR
                     PCRSLAVVIVDDARIAQLHRQFMADPTPTDVLTFDLRDDLINGPIEGEVVVSVETARR
                     QARRYRTEPDQELLRYAIHGTLHLVGFDDRTPSERRRMRREEDRILVQLAGKGPGTGK
                     RMRPRRARPRHPQETVGRA"
     gene            16115..17410
                     /locus_tag="P5316_09715"
     CDS             16115..17410
                     /locus_tag="P5316_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRR85276.1"
                     /translation="MLSIEFWIGLVLLLVLVLATTANTSVRHFSRLRLQKELEKRGRS
                     DLFPRLIDNQVALLFCSSLLRICCIIALVLLIEHVFRNTPAGARVGMDHEFLAHYGIT
                     FLSALLLVMVFGVAIPNAWARYDADRFLATGLPTLMVLQKMLLPLVSFQHAVNWIARR
                     LAGIPDDSDVQDEVEEIEKEILGVVSEGEATGVVHEKYASMIERIMQFRDKTVGQIMT
                     PRTDIVAIPSTATQEEAKELITREGHSRIPVYDENIDDIRGVLYAKDLLTLSEIDSFD
                     PLEMMRKVPFVPESKPIADLLQELREQKVHLAIVLDEYGGTAGLVTIEDIIEEIVGDI
                     ADEYETPAPESIRRIDENTIEVDARVRIDEINEQLRTELPEGEDYDTVGGFVFSALGK
                     IPETGEELSYQNVKFRVIDAEQRRINRLRVQVTPDRTQT"
     gene            17665..17738
                     /locus_tag="P5316_09720"
     tRNA            17665..17738
                     /locus_tag="P5316_09720"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17699..17701,aa:Val,seq:gac)
     gene            18509..20170
                     /locus_tag="P5316_09725"
     CDS             18509..20170
                     /locus_tag="P5316_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR85277.1"
                     /translation="MANYHHGPMAELAHQLALSPVRLRLRQLDAIEHLVDIIEPDKSY
                     PYDFVCNHITDYTPRGGLQRTMQGKSLIEDLVQMVEDLSSSPVLPAGIFRPCYTTEEL
                     AARLKVSTKTVCRWRRRGLPGRKLRYPDGTVRMAFLDRCVVRFVVRHRDLVRRGAAFK
                     QLSEDEKRRIVELARSILRERRVRLHELSQMIAAQIGRAVETVRYTLRRYDHAHPNEA
                     LFGNGDQPLVAPEMMAIYDLVAAGVPVTKVAEQTGRSESEIRSIVREVRVRQLAAKEI
                     SYIYNAEFDAPGADRLILGRHRIPEPARLKRVQPPKDLPPYLQDLYRQPLLTPEQERD
                     MFRRYNYVKFKADRLRKKLDATKATDGELDAVESLLAESERLKNEILRANLRLVVSIA
                     RRHVGRSSCFFEVVSDGNLSLMRAVEKFDYARGFKFSTYASWAIMRNYARSIPEQMYQ
                     SSKLVTGSDEALAVAPAPDTTSRDTMLEGARHLVQKGLQLLTARERDIVVRHYGLDDR
                     HDPMTLDQIGGLFGVTKERVRQIERQAIAKLRRALGDGQEAFVEA"
BASE COUNT         4007 a         6587 c         6028 g         3861 t
ORIGIN      
        1 cgatgtaccg ccgcctggtg aggcagtcga acatagccgc ggcggagggc gattacaccg
       61 ctgcactgga cgcagcgatg cggctgcgca gatcctggct cccggcggcg atgattgaac
      121 ggatcctcga ccccggcctc ctggatgcgc tcgtcggcgc ccggcttaac caactcggcc
      181 ggtgccagga ggccctggaa tggtgcgatc gaggattgcg tatcgcccgc aatccgagaa
      241 ccaaggccat tctccacgaa tgtgctgcga tggtccattc cacgatcggc gacgcgacgc
      301 gatgcgaaca gcacttaacc gccttggccg aagtactcca gaagcatcgc ataccggaga
      361 tcgaatgggg cgcaaccgcg atgttctgcg cgaacaacct tggccaactc gaagaggctt
      421 ggcagatcgc ccgccaggaa agcgatcggc gcggcgggca agtctccaac cgctgggtgg
      481 cggcggcgat ccaggcgctc gagaatctcg ggcggcacgc ggaggtgtta catctgaccg
      541 aatgggtcct ccaacagggc acgcagagga caaggtccat ccccgcggct cacaatcccg
      601 aggccgccgt cggcgccgcg gagcttcgcg cgagctacct gcgcaccatt cacgggttat
      661 ccttgctggg cggggccgag tccaccctgg agagcggaca atacgacctc tgcgagcgct
      721 atctcgccgt tctggagtcc atcgacctga aggaacctgt ccttcgtgct ctcctgaccg
      781 gcctgcgatc caagctgtcc gctcacacag gtgatcgcgc ggccgttgtc gacgccgcac
      841 ggaccatcga cgagctgaca gccaagtacc cggagcatcg gcgccttcga cccgtcgcga
      901 acctggcgct cgccgagtct tggcaaatac ttggcgagca tgagagagcc atctctctgt
      961 tgtctcaaat cgatccggcc gtgctgtcgc tggtggatcg atcgctcttg gccgcactga
     1021 tggccaagtc gctcgaaacc ctcggccaga ccgcacaggc ccaggcaaaa cgcgaagagg
     1081 cgcagcgact ggctccggcg gcatactgga accggccgtc cgaggccgct gacgaagccg
     1141 ccgtcgagac cgccgcgccg gggatcactc tgcccggcga gacgttgatc atcaggtccg
     1201 ctgtggaacc tctggcagac cagcccgtcg agcctgtggc atccggcacg gcgtgcgcgg
     1261 catggatctt ggccgtcccg gcgctgatac cccttgtggg aagcctgccg gccatcgccc
     1321 tgttcatcct gtcactgatc ctgctgtttc gcgacaaacg tcgactccac gaccgccgcg
     1381 ttggcgttgc cggactggcc atctccgttt tttccctggg ctgcgcaggt ctggccggat
     1441 tcaacgtcgc aaagatggcc ttggatcaca tacgcagcaa gcacgctgct gccaagaccg
     1501 agtattccga tgcggaacag gaggagtcgc aggatgcgcc tcccgtcact tcatttgctc
     1561 cggagcacga gcaggacgag acggacgaca ttcccgtgct cgaagagact gaggaacctg
     1621 cgccgatgcc cgcgagcacg ctgcccgagg aaggaatccg gaaacaaccg cagcccatct
     1681 ggcaccacgt cgtggttctt gcggtgctcg tggtgtcgat catcttccat gaaatcgccc
     1741 atggcgtcgc cgcctggtgg tcgggcgatc cgacggcccg cgacctcggc cggctcaacc
     1801 tcaatcccat ccggcacatc gatcccttcg gcagtatcat cgtgccattg gtgctcagtc
     1861 ttctaccggg agacaccgtc atcggctggg ccaagcccgt ccccgtccgg ccgcagaagt
     1921 accgaaactt ccggcgtggc aatctcggcg tctcgttggc cggcgtatcc ctgaacctga
     1981 tgctggccct cttcgcggca aacatcctca tggtccttat tatccttctc ggcgttgtgc
     2041 acccggccga gcggtatttc gacccggtgg cactgcttct ggggcgcctc catttgccgg
     2101 gcgtgcccta cgcctcgttg tgggtcgggg gaatcaagat ttgcacgacg gcagtgctca
     2161 tcaacctggc gctggcgaat ctcaacttgc tgcccctccc gccgctggat ggtttcggcg
     2221 cattgcacag cttcctgccc aacttcgcgt cggcgctggc taacaagctc ggtggggtgg
     2281 gcctgatcgt tttgttcttc ctgctggcca gcagaatagg ctacatcctg ttcgcgccca
     2341 cgatcgtggt cggcgcgctg ctcctgctgc tggtcatgtt tctcggcgga tgggcgattt
     2401 gagccggctt acaccggacc ggcgagcctc aattcacccg gcgcgggccc gtgcagcact
     2461 gtgagccaga tccagcgact tggcctcgcc gcacttaccc cgcacacgct gcggccaata
     2521 gcgggcggcc gtgaaagacc aatcgtactc gccggccgac aaacgcctcc agcactcggc
     2581 cggctccttc cagacgggtg acaacagttc gctcagcggc gcggcgttga tcagaacacc
     2641 gtcatcaggc tcaagctgcc aacccaccgt tcggccttcc tcgtccgatg cgctcaagat
     2701 acgctcgatc gcctcattca acgcctgtaa gtcagccgcc aacgagctat cacctcgccc
     2761 aatcgcctcg ctcaaccgac cggcaacatg ctgcgggcct cgcagccgtt cgagcgccgt
     2821 cacactgagc cgctcgtaat acaggtaggc gctataccca cgcccgcaag actgcaccag
     2881 ccaatagacc ggacgaccgt gataccgacg caaatggtca cgaaagaagc ggccggccag
     2941 gtatctgcgc agcccgacaa tcaaatcgcg atgcttgccg gttgcatagc cgaccacttc
     3001 ctccgtcccc ctatccccaa gcgtcatcct cagcacgcgc atcaccagct cggccacgtc
     3061 gtctccccca tcgcccgaca gctcagcaat cccgtcggag acaaccagtc tcctcaggcc
     3121 cgaggcagtg cgggcgtcaa agcggccaca accgagcgcc ccttggacgc cggggcgaaa
     3181 ccgccccatc accacgccca cggcgtagct aagccatcgg tgagccaggt cgacacgccn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctggtgagcc agatccacac
     3361 gccgctggtg agccagatcc acacgccgct ggtgagccag atccacacgc cgctggtgcg
     3421 agaatccgtt ctcgcacccc tcatctcgct cgtgcgagaa tcctttctcg cacccctcat
     3481 ctcgctcgtg cgagaatccg ttctcgcaca cttcatccgg cgaatccttt cctcccacct
     3541 cattgcgggg cgggaatctc gcgtcacccg tgtcgagatg ggaatctcga cacagcgagc
     3601 ctcccctctc acctcccccc cttggcaagc ggctcgccgt cctccccccc ttggcaaggg
     3661 ggggctgggg ggggtctccg ttcacatccg cctcgatagc cgcccgatcc tccgggccaa
     3721 gcacatacag cgtataaacc tcctcatcaa tcagccgctc gatctccgcc agttcctgac
     3781 gacgccgctt cgcagcttcc ggcccggttc tccaatccgg cggctggaca aactcccagg
     3841 tcgtctcatc ctccagactg ttccggcgtg ccagttcaat ggcccgcgag acgtgcgact
     3901 caagcgcctc caccgctgat ggatccgcct gcgggtacgg cagatcgccg acctcccgcc
     3961 caccgaaatg aaccgtcgga ttcagcagat cgatcacatg gcgggcgaag ctcgagttca
     4021 tgatcgccag cgtttccagc gacgacccgc ggccgtctaa ctgaaacgcc gccatgcctt
     4081 ccacattgca gataaacccc ggcggcagga tacgaacgct caacccccgt gagctggtgt
     4141 gggtccaggt gacaccggac cggaagtaat agtccatgtt ctgcggccgc gacgccacct
     4201 tgcctgattc gatacccagt gtgcgaatct cggccccgtc gttcgcccaa ttaacgacgt
     4261 attcctggtt gccgtaccag cgacgaaagt cgccaccctt gatatgcggg aaccacttgc
     4321 ggccggtggc cagggcggcg gcgcgatcgc ggcaaccgaa agcaatgcac cgattggcgg
     4381 caaccgcatc atcgggatca tctcgaaaga acacttccca ccagaaacgc agaaagcgga
     4441 aattgtcact ggtctgcaaa cccactgtcg gccgggcgat ctcacgcagc ctcggcaggt
     4501 ggtcgaacag acctcgcacg cgaggcgtga tccaaaacac ccagggccgg ccaggcaaat
     4561 gatcgaaatc cccttgtcgg cagcggaaga ctcggctgtc ggcttccccg cgccgcaaca
     4621 cggtaaccgc ttgctcgaac gcctgttgct tggcctgcgc gcccgaagcc cgggtgaggc
     4681 ggaagaatgt ccccaagcag tcgtcacggt tcccagggtc tgcctcgcgg cggagcacgt
     4741 agaatgcggc atcgaccacc gcgtcgggaa acgtacccag cccgccttgt acgagcgtct
     4801 cgatggcaac cgaacgacgc agcacatccc gcaggtcggc aaaactcgat atgaacatga
     4861 acgtctgcgg ggtgatgata cctaaccgcg cgccttcggc gagaaggcca acgcatcgct
     4921 caagaaaagc accatagagg ttgcgtttgc cggccggata agcattggcg atgaatgact
     4981 tgagcgaggc gtcgtaatcc cggctatcga gatacggcgg attagtcacg acaacgtcat
     5041 aggtcgcctc gaaaacatct gtggtcgctt ccccaatctc cctgttgtgc tcccacaaca
     5101 tgcctcccgc aagccgcaaa gcatcggcgc agtacaaatg cagagggact atcggcgtgt
     5161 tcggagtaag agaaaccgcc ttcagataca acgtcacggc cgacagtcgc acggccaagg
     5221 gatcaatatc gaaaccgaac agattattgg ccagaatcgc ggctggaatc gccgacttac
     5281 cggcgaccga aggtcgatcg ggccaacctg gctgcccggt gtgctccagc tcctcgcgat
     5341 acatctccgc cagcaaatcg aagaccacaa gcccgaagtg catcgctccg caggccggat
     5401 cgaggacgcg aatctcgcaa gccggcttca gcttcgcatg cggcagcggc tcagccggcg
     5461 ggacaaggta ttctaaccgg tcggccaggc gcgaatcggg atgcatctgc aaccacagcc
     5521 ggccgagggt gttctcgacc aggaaccgca cgatccatcg gggcgtgaaa agctgcgtct
     5581 tggctgggag cagctcggcc gtcaccttcg gcggacgcga accgcgaaag accgcgatgt
     5641 ccggctcgtt gaagtattgg tagaaccagc ccaggctctc ctcgttgccg ggctgccacg
     5701 cctccgcgag aagaggctca tccagaatcg cgtcaagtgc ggacttggtg tccgatgact
     5761 ccccaaccct cgctggggat gggacggaag acgctaacaa tgagtaccgc tcgtcccgga
     5821 atcccctccg ggccgtcttt cttgacgaaa tccgttccac agttggcaaa gacgaggatg
     5881 agaatcctcc acctggaagg ccccgaagag gattctgggg cctgccgaca ctcgcatcac
     5941 gaggaacgtc ccgccctgcc accccccctt gccccccctt gccaagaggg ggagtctcgt
     6001 tgagcggcag caggtaatca aggactccgg catccaggcc gcgggcttgt agcattcggt
     6061 tcgcggcggc cctcagaaga tgcgtcgcgg ccgccttgcg cgtctcgatc ctgccgggct
     6121 ccgcgcccgc cgcgcgcaga gcctccatca gcaccgcacg acaccgcaag gccgcgtctc
     6181 gaatggcagt cgtatccaat ggcagctcca gatcattcaa caagagcaat caggagcatc
     6241 agactacaca gaccgaggcc gtgtcaagca cccgcgatct gtcttgacga gcccagctgc
     6301 ttattccggg agcgaacgga atcggcgtgc aaagcccgcc ctaccagatg taggccgagg
     6361 tgatcctcgc atggatcaat aatcgcagta ccacccgtcg caatagcccc aatccatgta
     6421 atcatagtag tcgccgtagt cgtagtagtt cgcgtagtcc gagtaattgc catagttgga
     6481 gtaatcggag tagttaccat agttcgagta gtcagagtag ttcgcgtagt cgtagtagtc
     6541 tgaatagtca tagtagtcgg cgtagttgcc gtagtcgcag tagctgccgt cgcagtgcga
     6601 gtagtcctgg cctgcaccgt cgcagtagct gcaagcgtcg tcgccgcccc agtcggagta
     6661 gtttccgtag tcggagtaat cgttgtagcc gaaatccaga tagtgatcgt agagccagcc
     6721 gccgcttgcg tgatcgtcat caggctcaat atcgatccag catccctgcc caagcgagcc
     6781 cagcgcggtt ccgatggctc ccagcttgat caaatgcgag cccagcgtgc ggaaaaacgc
     6841 ccggcggctg atagggctca gaggctgctg gtccggctct tccggggtgg ccatgaatgc
     6901 acccggccgt aagcccggca gagtaaaggc ggcatccaat cagacacgcg aaccagcaga
     6961 aggttggctg cgagacagcc cgttccaccc tgtggcaacg gcatcttacc ggtgagcaag
     7021 caccgttctt cagggacggc attataggcc gttttcgcct tcagtcagat tcccctgttg
     7081 agtcgactca agcccaatcg tacaatgcat ttgcagtcgc ggaacgaatg aagaggagaa
     7141 tggcctgatg aaccttcttt gcgccggcac actctccaaa ggatctctcc gtgtccagac
     7201 atgtccgcgc catttcgcgc gggctttggg tatcgtcctg gcctccctcc ttgccggctc
     7261 gtctgcagcc tcggcccagc cgaccgagag ccggctcggg ctgcacatga tcatcaacta
     7321 cacgagcggg gcttcgcagg tagtcaacgc ccacccacga atccacaaga tcctcgacac
     7381 ccacggcgcc atgctggcgt cagcacgcga tttcaagaac aacaacccca acggcgtgct
     7441 ggtcctgcga atctacacca cgaagtcgta caacatctcg cacgaccctg cgtgggcagc
     7501 cacagatttc tggaacacgg tcctggcccc gcccatcaat agcctgtcag cccaggaccg
     7561 ggcgctgatc gactacgtcg agggacccaa cgaatgcgac agcaccccct gctgggggtc
     7621 cattcagaat gcgcagtggc tcaacaactt ctggctttct ctggctcccc tcatcgccaa
     7681 cgccgggttc cgcccctgtg ccttctgcat tcccgtgggc aacccgccgg gctcgctgag
     7741 cgatatgcag gcccagctcg atgccgtcgt gccggcgctc cgggtctgca aaagctacaa
     7801 cggcgggtgg agttatcacg catatactct tccctactcc aagagcgtga gcacggagat
     7861 ctggtactcg ctgcgatacc gccagtttta cagctacttc cggagcacct accccgacct
     7921 ggccgacctg ccactgctgc tgactgaagg cggcgtcgac ggccagagcg gcgagggcgg
     7981 cccgggatgg aaggtcgacg atgcggcccg gtacgtcgac tggctgactt ggtttgacgc
     8041 tcggatgaaa gaagaccctt acgtcgtcgg ttgcacgttg ttccaatcgg gcgacctgtc
     8101 cggctggttc tccttcgaca ccgaggaaat caacccctgg ttggcccagc acatcctctc
     8161 gtctgttcct ccgacgccgc ccgccgtacc cgccaatctc aacgccgcga tcaacggccc
     8221 gacttccgtg caactgacct ggagctctgc aagcggtgca atcagctaca acgtgaaacg
     8281 atcaatgacc ggcggagact cctactcgac catcggaacg tccaccacgc tcagttacac
     8341 cgataacgac ctgacgctgg gagccactta tcactacgtc gtcagcgcgg tcaataacgt
     8401 gggtgaaagc gcgaattcga acgagtccac catcaccgtt acaggcggat acgcggccaa
     8461 ttcaggcggt tcctcggccg gcagattcgc tgccgacaca tgctacgacg gcgggtacac
     8521 ctatatgacc agtgcgtcgg tcgatacaac cggcgcgacc gatccggcac cgcaggccgt
     8581 ctatcagtcg gagcggtggg cgtctccgtc gttcacttac accttcccca atctcgtcgc
     8641 cggtgccgag tacactgtcc gcctgcactt cgccgagatc tacttcacat ccgccggcaa
     8701 acgcaggttc aatgtgagca tcaacggagc gcaggtgctc accaattttg acatcatcgg
     8761 cacggccggt gcggccaata aggccgtcgt tcacacattc acaaccaacg ccaacccgag
     8821 cggccaagtc atcatccagt attcagcagg ctcggccgac aatcccaaag ccggcggcat
     8881 cgaaatcatc cgcaacctcc cgccggtcta tatgggcgat ttcgatcagg atctcgacgt
     8941 cgatcaaaga gatttcggcc tgctccaggc gtgcctgagc ggaaatggca tcacgcaaaa
     9001 cgatccttca tgcaccgccg ccgatctgga cggcgatgcc gatgtcgacc tggctgattt
     9061 cggcgtcttc ctgaactgcc tgagcggcga gcgggtgatg ccgccagaaa gctgccgcac
     9121 gtagcggcgt ccctcgctgg tttcctcgtc gtttgaaagc gttgcgctct gggataaccg
     9181 gcgggataaa cccgcttgac gagccgaggg gtttatcccc tcggtcctct tgacgccgtt
     9241 cgttcgctgg tcaggcagaa gaccggcggc ataaagccgc cggctcgtga agacacgggg
     9301 tttatcccct cggtcctctt gacgccgttc gttcgctggt caggcagaag annnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgtgaaga cacggggttt atcccctcgg
     9481 tcctcttgac accgttcgtt cgctggccgg gcgagagacc ggcggcataa agccgccggc
     9541 tcgtcaagac agcatatccc tcgggtatca cttgcgcgcc gactaccatc ccttgcgata
     9601 tggcgggtcg atgtactgat tggcctcggg aatgttcgtg atcttgcgat tcttggcgtc
     9661 ccactcgatg cgtttgccga cgcggacggc cacggtcccg agcagtagcg actcggtcat
     9721 cacgcttgcc cggccgaaat gactcatgta gtccaggccc aggtcgccgt tcttgcagtt
     9781 gcgaatccat tcctcgtgat gacccggcga gtcgggaatc gtccgcggcg gctgcttgag
     9841 gtccttgaac ttgtcttcgg gcagcagtac cctggcagcg ccgtcggcac ttggcatgaa
     9901 tagcgtacct ttctcgccga cgacgatcat gccgttgtca ggaagctcgc ggttctcgat
     9961 cagctccggc gagggcttct tgccgccatc gtaccagtac aggcgaacgc cgggacgatc
    10021 ggaattgccc ggaaactccc atttcacctt gctccatgca gggtaggtct cctcatacgg
    10081 ctcagagcac tcgcattcga tcgccgtcgg atcgcgcaga tcaaggcccc agaaagccac
    10141 gttgcagatg tggcaagcca tgtcgccgat ggcccccgtg ccgaagtcat accagccccg
    10201 ccacttgccg ggcagatacg cggggtggta cggccgctgg ggggcgacac cgagccacag
    10261 gtcccatttc aacgtcgccg gcaccggcgg cgtctcgatc ggacggtcaa gggcctgttt
    10321 ccagaacgta cccggccgat cactccaact gtgtatctcc cgaaccgtcc ccagtacccc
    10381 ggcccgcagt agctccacgt tgcgccgggc accgctttgg gcgttgcctc cgttgcccat
    10441 ttgggtcacc accttgtgct tcgcggttgc ctcggcaaca cggcgagcct cggccaccgt
    10501 gcgaaccagt ggcttctcgc agtagacgtg cttgccgagt tcgatcgcag caagcgtgat
    10561 cggagcatgc atgtgatccg gcgtgctgac tacaaccgca tcaatctgct tgtgcagctt
    10621 gtcaaacatt acccggtagt cggcgaactt cgtaacattc gaaagccgct tgaaggtctg
    10681 cccgccgcgc acctcgtcaa cttcacacaa ggcaacaatt ctctcgttca tcattgcccc
    10741 aacattgtcc gcgccacgac caccgattcc cacacaggca acgccgagct tctcgttggg
    10801 cgacttgccg gcggcccgcg ccggccggcc ggaaacccag aaacccgctc cggcggctat
    10861 cgtggtcttt agaaagcgac gtcggctcgt tttgttcgtc atagggtcct cctgagatac
    10921 caaccacggc ctcaacccgg cgggtgactc catagcatcg ccgccggctc gtggacgtcc
    10981 cgggccggct cgtttagcat agcgaacaca gaggtgaaaa acacatgctc tggcaacgta
    11041 ttgcgttcgg gaccttgatg atcgccgtcg ttgtgggcct cgcctggctc gatggctggc
    11101 tttcggcgtg cggcttaacc tcgctcgatg aaggcgtgct tggcggcgct accaggatcc
    11161 ggctgatgtg cgggctggcc ctgacactgg ccttggccct gctgatcatg ctggccgttt
    11221 tcgagatggg ccggctcagc cggcaaggag gccacaatcc ggccaccaac tgggctgcgt
    11281 ttgtgtcggt cctgctgatg ctcgcgcctt ggatcgaggc tcagcagaag ctgtcgtccg
    11341 cggtgcctgc tctccgcctc acaggaggca tgccgctctc actgttcctc cttaccggcg
    11401 gcatcctggg agcctccctg tgtatcctcg cacgcaagac caccgagagg gccttctcca
    11461 acttggccgt cacatgctta atgatgctct atctcggact cctgggctca tttgtcatcc
    11521 gaattcgttg ccttgatgcc ggaccagccg gtgcagcctt ggtcatcttc ttcatcctga
    11581 ccgtaaaagc cgctgacatc ggggcgtact tcacaggcat cgcctgcgga aaacacaagc
    11641 tagcgccttg ggtcagtccc ggaaagacca tcgaaggggc tgcaggtgcg gttgtccttg
    11701 cggcttttgc tgcctgcgcg gccatctggc tttgggagtg ctgcctggac tccactcttg
    11761 ggccgccccc tttcagcctc gcgcaagccg ttatttttgg gctggttacg gccgtttgtg
    11821 gccacctggg agacctcgtt gagtcgctga tgaagcggga tctcggcagc aaagactccg
    11881 gacaagtcgt tccggccttc ggcggattgc tggatatcct ggattcacca gtgtttacgg
    11941 ctccgatcgc atggtggttg ctgacattct tcggccgtat cggttaaaat agagttgtcg
    12001 ggcaaagccg gcgacttagg ccggttcgga ccggctgggt cgttggactg ccttgttgac
    12061 gggagatagc aggcaagttg gaacttacca taacgctgaa cgacgctcgc aagcggaatg
    12121 cccttttcgg ttcggcagac cgccatctga gacagatccg gggtgcgttc ggcatacagc
    12181 ttagcgcaag ggactcgacc gtcaagttgt caggtgccgc ccacgccgtc ggacaggctg
    12241 catcggtcat cgactttctg caacgtgcgg tccgcgattc ggacgaactg acggacgagc
    12301 aggtctccga ggccatcggg tacgtcatcc agttgaacaa tcccgagaac cgcgatgagg
    12361 atatcatcga ggtctacagc cgtacgaccg tcacgcggcc ccggacagat ggacagcgag
    12421 cctacgttca ggccatgcgc ggccacgatc tggtcttctg cgtcgggccc gccggaagcg
    12481 gcaagaccta cttggcggtg gccgtggcag tctcgatgct caaggccgga accgtcaagc
    12541 ggatcgtact ggtgcggccg gcggttgaag ccggcgagaa actcggcttt ctgcccggcg
    12601 acatgcaggc caaggtcaac ccttatttgc gcccgctctt cgacgcaatg ggcgacatga
    12661 tgaccttcga gcagatccgg cgattcatgg aaaatgacgt catcgagatc gctcctctgg
    12721 ccttcatgcg aggtcgaacg ctcaacaacg ctgttgtgat tcttgatgaa gcccagaaca
    12781 cgacgcggct gcaaatgctg atgttcctga cgcgactggg acaccacagc aagatgatcg
    12841 tgacgggcga cgacagccaa atcgacctcc tggatccgga gtcgagcgga atcagcgacg
    12901 cgttgcgacg tcttcggtgc atccccggga tcgccattat caggctggaa aagcaggaca
    12961 tcgtccggca ccggctggtt caggatatcg tcaacgccta cgctaacggg aaaccatgat
    13021 gttatgggac gcggccaggc gggtctttcc ccgcgccggg tctttgtcga tgaaatgccg
    13081 tgtgtgcaga gcgggacgat ggcgcaaccc ctgacaccgg cactcgcggc actgttgccg
    13141 aggttctctt agaatcgtga gttcgcgacc gtcacctgta tccgaatcgc gacgccagga
    13201 agcccgtcgc cagcgcgacg agcgacggga gaccctctgg atgagcctgc gacgcagggt
    13261 tcgcccctcg tcattgctga tgggggtggt tttctacatc ggcgtccttg tggttcaatt
    13321 ctggggcagc gatacgatgc cctggagcct ccagcaactc gtcgaccggg acatcaacgc
    13381 acgcgtcgat ttcttctacg aggatcaggc ggcgacagaa gccaaccgcc agaaagcgcg
    13441 cgacggggcg ccatccgtct tcgtgttcga cgccggtacc gcgcaggtgg aacctcagac
    13501 ggtgattcgc actcagatgc atgagctgcg cgaattggtc gccaaagccg agaactccga
    13561 cgccaaggcg atccgggacc aagcggccgc caaaggttgg atgctggacg acggcgcggt
    13621 gtcggcactg cgggctttcg caccagccgc caggaaggct gagtatgagc ggatggtcga
    13681 tgccctcctg tcacggctgt tgatcgtcaa cacacccgaa gatccgaacc gcaaatcgac
    13741 gcctccaaaa gtgttgctga gaacgaagga caagaacggg gccactaccg acaaagaagt
    13801 aggtagcgat ctgctcattt atctgccgaa agcaaaggat acaaagatcg atgatcagat
    13861 catggcagcg atgcacggcg ttcgctcctg cccggaggcg ctgcacaaac cgtttgcgga
    13921 tatcgtcctc aaggtagtcg ccggaccggc caaagacaag ccttcctaca ctccgttctg
    13981 gcagtacaac gcggccctga cggcggagca aatgaacgcc gccgccgaac tcgtcaaaga
    14041 ggaaatcgtc tcctacaagg cgggaacgac cctcgtcggg gccaaaaccg tgctcggacc
    14101 ggccgagctg aacctgctga agcacgaaca caaagcctat cttgaggctc agaaaacgga
    14161 tcctgtcctg cggcagcgga gactgttgcg acagctcggc acggccgtcg ttgtactgat
    14221 cgtggtgacc gggtttgccg cctacgtcgt gtcctaccgc cccaggatta ccgaggttcc
    14281 ttcgcggaca ctggccctga ccggtttgct cctgaccatg atcgttctga gccacttggt
    14341 ggaccgtctc cagggctttg gcagcgactt tccggccgag acctgtgtct ttttcgtggt
    14401 tattgcctcc gctctgctga cgatcgccta cgaccagagg ttcggcttcg gcgtcgcggc
    14461 ggtgctgacg gtgctgacga cgctcaccgt agacggcgat ttcaacctct tcctcaacta
    14521 catgacggcc gcaggcgtca ccgtggcact gctgaaggac attcgcactc gcagcaagat
    14581 catcgccgta ggggcgatca ccgccgtcgc caccgccctg gtgtcggcgg cgaccagcct
    14641 tgtggccgag caaccatggc tctatatcgt gacccgcgcc gccctgtcag ggagcatggc
    14701 tctcttcgcc ggcttcatcg tgcagggcat tctgccgcat ttcgagaaag tgtttggcgt
    14761 tgcgacgagc atgacgctgc tcgagtggtg tgacgcgagc aagcctttgc ttcggcgcct
    14821 ggcccaggaa acgccgggca cgtacagcca ctcgctggtc ctgagccaga tggccgaaga
    14881 ggcctgtgag acgatcggtg cccacggcct gctggcccgc gtgggcgcgc tctatcacga
    14941 cattggcaag gcccagaaac cggattactt cgtcgagaat caggaggcaa ggatgaaccg
    15001 gcacgaccgc ctctcgccga ccttgagcct gctgatcatc gttggacacg tcaaagacgg
    15061 cattgagatg gcccgcgcct atggtttgcc gcgtgtgctt catcagttta tcaacgagca
    15121 ccacggaacg actgtggtac gatacttcca tcacatggcc agcgaggcgg ccgcgaagag
    15181 cccgttgaag ggcaagcacg accgtcaggt ttctgaaagc gagttccgct accccggtcc
    15241 caagccgcga tcaaaggaat cagcaatcct catgctgtgc gatgcctgcg aaggcgccgt
    15301 ccgggccctg aacgagccca cgcccggccg catcgaaagc acggtccatc acgtggtcat
    15361 ggaccgtctg aacgacggtc agttcgacga ctgcgagatc aacctccgcg agttgagcct
    15421 ggtcgagcag tccatcgtca ggagcctgtg cgccattcat cacggacgga tcaaataccc
    15481 gaaggccgat cgcccggcgc cgccaagagc tcccgtcccg gaaaacgaag ccggcgcgat
    15541 gcgcaacggc gcggccgacg tgccggaggt agtcgagcag gcgtgagaac caagacacac
    15601 gcaacccaga ccggttctgc tgattgccgc atcacggccc gccggttcgc cacatgccac
    15661 gaggtcaaca ccagccggat cgtgcaggca gtgcgggccg cgctcggtaa ccggccgtgc
    15721 cgcagcctgg ccgtcgtcat cgtggacgac gcccggatcg cccaactaca ccggcagttc
    15781 atggccgacc cgactcccac cgatgtgttg acctttgatc ttagagatga tctcatcaac
    15841 ggcccgatcg agggcgaagt tgtcgtttcg gtcgagacgg ccagacgtca ggcgagacgg
    15901 tatcgcaccg agccggatca ggagttgctc agatacgcaa ttcacggcac attgcacctc
    15961 gtcggcttcg atgatcgtac gccctccgag cgacgacgaa tgcgcagaga ggaagatcga
    16021 attctggtgc aactcgctgg caaggggccc ggaacgggaa aacggatgcg gccgcgccgg
    16081 gcaagaccca ggcatccgca agagactgtc ggtcgtgctt agtatagagt tctggatcgg
    16141 gcttgtcctg ctgctggtgc tcgtgctggc caccaccgcc aacacgtcgg tgcgacattt
    16201 ctcgcgcctt cggctgcaga aggaactcga gaagcgcgga cgttcagact tgtttccccg
    16261 gttgatcgac aaccaggtcg cgctgctgtt ctgctcatcg ttgctgcgca tctgctgcat
    16321 catcgccctt gtcctcctga tcgagcatgt cttccgcaac accccggccg gcgctcgcgt
    16381 cggcatggat cacgaattcc ttgctcatta tggcattacg ttcctcagtg cgttgctcct
    16441 ggtcatggtg ttcggggtcg cgattcccaa cgcctgggct cgctacgacg ccgacagatt
    16501 cctggccacc ggtctcccga cgctcatggt cctccagaaa atgctcctgc cgctggtctc
    16561 ctttcagcac gccgtgaact ggatcgcgcg aaggctggcg ggcataccgg acgacagcga
    16621 cgtccaggac gaagtggagg aaatcgaaaa ggagattctg ggcgtggtca gcgaaggcga
    16681 ggccacggga gtcgtacacg agaaatacgc gtcaatgatc gagcggatca tgcagttccg
    16741 cgacaagacc gtcggtcaga tcatgacccc tcgaacggac atcgtcgcga ttccctcgac
    16801 cgccacccag gaggaagcca aagagctcat cacccgggaa ggacactcgc gaattcccgt
    16861 gtacgacgag aacatcgatg acatccgcgg cgtcctctac gccaaagatc tgctgaccct
    16921 cagcgaaatt gacagcttcg accctcttga gatgatgcgc aaagtgccct ttgtgcccga
    16981 gtccaagccc atagccgacc tcctgcagga actgcgcgaa cagaaggtgc atctggcgat
    17041 cgttctcgac gaatacggcg gaacggcagg actggtgacc atcgaggaca tcattgaaga
    17101 aatcgtcggt gatatcgccg atgaatacga gacgccggca cccgagtcca tacgccggat
    17161 cgacgaaaac actatcgagg tcgatgcccg ggtccgcata gatgaaatca acgaacaact
    17221 gcgcaccgag ctgccggaag gtgaggatta tgacaccgtg ggcggtttcg tcttcagcgc
    17281 cctgggcaag atccccgaaa ccggcgagga gttgtcgtat cagaacgtga agttccgggt
    17341 tatcgatgca gagcaacggc gcatcaaccg gttgcgggta caggtaaccc ccgaccgaac
    17401 ccagacatga acagtcccac gagccggctc cccgccccgc tcggggaagc ctcagctcca
    17461 tcgggagaca aacctcaatg gcgggtgcgt gacacactgg ccgggggtac aatgtcggca
    17521 ccacgggcgg cctgtccgca cgtcggggcg gcataagacc ctggcaggcc ggcttccagt
    17581 gtgactcctc cctggactct cctggagcct caatgccggc agttattgac acgcatccgc
    17641 caatcgcctt aaatagagac ctgcgggcga ttagctcagt tggctagagc gcttgctcga
    17701 catgcaagag gtcactggtt caagtccagt atcgcccaat cgatcgtaat tcccgccgaa
    17761 ttcgcgagtt gcgaagaccc gttgtccggc ggagcggccg taaccgaagg cccaaatgcg
    17821 agtagtacta ctcgcgaaac ctcggaggtc ggtcgtgtcc gccaaaccca agcgaaggcc
    17881 gcgctaccgg atcgacaagg ccacaggtcc aggctcagac ctcctgatct cggccgttgg
    17941 tctgattttc ccttgcgggg atccatggtt tctatcgacc tctgcggttg cgagctctgg
    18001 ccaaacccgc gatcaaaccg ggccattcag cgattgccgg cgcacctcaa tgccaccggg
    18061 ttcccctgcc caggcaccaa cctccgactc gaatacgaac cgaccgccgc cgctccaacc
    18121 catcgcgcgg cccgactttg gtttccgatg agatccggag gtttgagcct tccggcgccg
    18181 caggtggcgt tccacgtaaa atcattatcc acagcttctt gcgcctgcag gtgccgtggc
    18241 cggcaggtct tgccccgaca atcataaacc gttgtcagga ttgacttttg ggcgcgggtg
    18301 atcgataata aattgagacg cttgccgggg gagcttgcgg ctgacggcag gtttcccttc
    18361 gacgagggtg gcggcccgag agcgatgagg ggctctatgg tggctgatgc ttctcggatg
    18421 gtcacctctg ctggtgacct tgagtcgggt ccaaggctga ctcggcggca acgtttcgag
    18481 tgaggctctt gcctccggga gacggaccat ggccaattac caccacgggc ccatggcgga
    18541 gctcgcccac cagcttgcgc tgtcaccggt cagactgcgt ctcagacagc ttgacgccat
    18601 tgagcatctc gtcgatatca tcgaacctga caagagctac ccatacgact tcgtctgcaa
    18661 tcacattacc gactacactc cgcgcggcgg actgcagcgc accatgcagg gcaagtcact
    18721 tatcgaggat cttgtccaga tggtggagga tctgagttcc tcgcctgtgc tgccggccgg
    18781 catctttcga ccctgctaca ccaccgagga actcgccgct cgcctgaagg tttcgaccaa
    18841 gacggtctgc cgctggcgtc gacgcggatt gcccggacga aagctgcgtt atccggacgg
    18901 cacggtcagg atggctttcc tcgatcgatg cgtggtccga tttgtggtac gccaccggga
    18961 tctggtacgg cgaggcgcgg ccttcaagca gcttagtgaa gatgagaagc gacgaattgt
    19021 cgagctggcg cgatcgatcc ttcgtgagcg tcgcgtccgt ctgcacgagc tgtcacagat
    19081 gatcgccgcg cagatcggcc gagccgtgga aaccgtccgt tatacgttgc ggcgatacga
    19141 tcacgcccat ccgaacgagg ccctgttcgg caacggcgat cagccgctgg tcgcgccgga
    19201 aatgatggcg atttatgatc tcgtcgcggc gggcgtgcct gtgacgaaag ttgcggaaca
    19261 aaccggccga tcggaaagcg aaatccgatc gattgttcgc gaagtccgcg tccgtcagct
    19321 cgcggccaag gagatatcct acatctacaa cgccgaattc gatgcccccg gagcggaccg
    19381 tctcattctc ggtcgccatc gcatcccgga gccggcccgt ctcaagcgcg tccagcctcc
    19441 gaaagacctc cctccttacc ttcaggatct ctatcgccag cccctgctca cacccgagca
    19501 ggagcgagac atgttccggc gttacaatta cgtcaagttc aaagccgacc gacttcgcaa
    19561 gaaactcgac gccactaaag ccaccgacgg cgaacttgat gccgttgaat ccctcttggc
    19621 cgagtcggag cgcctcaaaa atgagattct gcgggccaac ctgcgtctgg tcgtcagcat
    19681 cgcccgccgg cacgtcggtc ggtcttcctg ctttttcgag gtcgtcagcg acgggaatct
    19741 ctctctcatg cgagcggtcg agaaattcga ctacgcgcgg ggcttcaagt tcagtacata
    19801 cgcctcctgg gccatcatgc ggaattacgc ccggagcatc ccggagcaga tgtaccagtc
    19861 cagcaagctc gtgaccggct cggacgaggc cttggcggtc gccccggcac cggataccac
    19921 gagccgcgat accatgctcg aaggtgctcg tcatctcgtg cagaagggtt tgcagctgct
    19981 gaccgcccgg gagcgcgaca tcgttgtgcg acattacggc cttgacgacc ggcacgaccc
    20041 catgaccctg gatcagatcg gcggcttgtt cggcgtcacg aaggagcggg tccgccagat
    20101 cgagcgacag gccatcgcga agctccgccg ggctctgggc gacgggcaag aggcgtttgt
    20161 tgaggcctga tccattcaac tctgctgacc ggcggtccaa cggcctgatt cacgaccttt
    20221 tgccggatgg ccggtgcaga cccgtctgaa gcaaacaaga acacgacacc cagaaggcgt
    20281 tgctggctac ccctgtttgt tgccggtgat gttccaggca agggcacggg tcgggttgtt
    20341 gtcggcgggg gtgatgggaa tgaccgccgt cgaagggact gaggcggttg gcggggcacg
    20401 tagctttgag ggccaaggtg tcagcccggc gaagtcgtgg aaggccggtg gcgagatcgg
    20461 gcgctgtgtt ctgggtggat cggatgacgg ggaaccggtt gggggtttct ttaagtcttt
    20521 aagctttaag gacttaagat ttgcagttgg gtttgtttgg cgcgggggag tggtcagggt
    20581 ggcggcaggt cggcgccctg ctacgtcggg gcgacttggc ggggctctga cctgccctgc
    20641 ctgcttcagg attcttttgg aggcgcatgc ccacgcaagg ccg
//
LOCUS       DAOTPW010000088        20606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2660_length_20606_cov_5.366989, whole genome shotgun sequence.
ACCESSION   DAOTPW010000088 DAOTPW010000000
VERSION     DAOTPW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20606
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2660_length_20606_cov_5.366989"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="P5316_09730"
     CDS             <1..904
                     /locus_tag="P5316_09730"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRR85278.1"
                     /translation="LLAAWLLADWPAGRFVFLDKWAPSWRPWLRGVVEKAPVGLSIMA
                     GALALHIVGLIDDRRPLSARVKLLIQAAVTLAITAGVGVRSAEALGPLPAVLLTTFWI
                     IALTNAFNFMDNMDGLSAGVAVLTAVVLAVSSMVAGQVFVPCVLMLVAGGAAGFLIYN
                     FPPATIFMGDAGSLVLGYMLAVCAVMTTFYDPEMGTTPFGVLVPVVVFAIPLYDMISV
                     IIHRLRLGVSIFRGDRRHFSHRLVKRGLSPRTAVLTIYLATLATSLPAVLLPVHGWVA
                     ALLIVAQCLAVVTIIAILESCNES"
     gene            894..3302
                     /locus_tag="P5316_09735"
     CDS             894..3302
                     /locus_tag="P5316_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRR85279.1"
                     /translation="MNREPAPNRRPPTGVAPVAGRVPHALDGMAFLVLLLIVSMRALL
                     SETYESGLTGVSQAVGDIASLTPATTVMLDTAIWVAAAMAALATLLRSRSWQRTGIEA
                     GWAIMAVAAVCSCCVASNKRLAINASCDWLTTPVLAITLANLLRERRRVVLVLAVVVA
                     SGLASATKCGSQLGWEFGDTWQAYQEQKAEFWGRQGVALTDPTVELFERRMMAREATG
                     FLPYSNAQGAGLILAGFAGIALAFLSGRSRTAKGVCGVVAAVILASVVTTGSRGAMLA
                     ALIGLALLIAGTLIGSRWYQKWKALWAGLWITAAIGVALVVIWGTTRGRLPGDSLRFR
                     WEYWRVTSKIVAEHPWTGVGALNFDRAYLKHKPVQYPEEIRDPHNFAMAIVSQWGIIG
                     GAGMMIALLGGSWVAIRALGQESASPPEAAQPKEPVVQPLGKWVVAVIVGFVLFRLWM
                     LRGWLDVGEGGVATVLFDLLLYGLVWCLAFAGLSLLLSFGWDDADMGSASTAPQANKR
                     HWPSQKAHQQLSPPYQGGGRGGDGYRLPCLIALGVFLLHNTIDFSLFVPGTLTTFAAL
                     TAVAVARAAGQGEGSGAARRWLPMVASAGGLLAVIILLVAPVLRCTSSLKDARFAVAT
                     GLRDPAPLYKAAAEADRFDPTPLTEWASWAAHLDVTGLDKSLTCLQMAQDRDPLDRGI
                     YRSQWRVLELRYDAAHNPADLLQAVAAAEKAVDLYPQSPDGHADLARLLGRVASEIAS
                     DPAAEAVGRDWLADAVMHYRRALHLDAARPSYEIRRWHHDRRMQIEGRLSQLERMASA
                     RAGS"
     gene            3591..4595
                     /gene="ilvC"
                     /locus_tag="P5316_09740"
     CDS             3591..4595
                     /gene="ilvC"
                     /locus_tag="P5316_09740"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRR85280.1"
                     /translation="MAVKIYYDKDADLGALKGKKVGIMGYGSQGHAHAQNLRDSGIEV
                     VVAELADTPNYRLAVEHHFKPVSVAEATKACDLLVMTLPDEVQAAVYRKEIAPNIRAG
                     QALGFTHGFNIHFGQIVPPEGVDVIMIAPKGPGHLLRSEFVKGGGVPCLVAVEKNATG
                     KALQTALAWGSGIGGGRAGILETTFKIETETDLFGEQVVLCGGVTALVKAGFETLVEA
                     GYPPEICFFECMHELKLIVDLFYQGGINYMRYSVSNTAEYGDLTRGPRIITDQTRAEM
                     KKILAEIVNGDFAREWLLECSVGRPSFNALYKKDYDHPLEVVGRKLRKMMKWIDSKEV
                     "
     assembly_gap    5138..5237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5393..5935)
                     /locus_tag="P5316_09745"
     CDS             complement(5393..5935)
                     /locus_tag="P5316_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85281.1"
                     /translation="MKSDRQFGLLITWTCYGTWLPGDSRGYVSNLQVNPTRWLPKENA
                     PGSPCRSDDPYAHAQASRRQQHKTIHLDRRQAACVAASLVETARSRAWRVLSARIMAN
                     HVHVVVTDCPNDGPAVRRILKGTTQASLNRLAGAPHKWWTRGGSNRYLNGSDHIEAAI
                     KYVEDQEFVLAGVDDMKVLP"
     gene            complement(6362..7375)
                     /locus_tag="P5316_09750"
     CDS             complement(6362..7375)
                     /locus_tag="P5316_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HRR85282.1"
                     /translation="MSDSQDPDSMPSTSATQPGREAGGLGRVLLTGASGFVGGYVLRE
                     LVDQGYRPVCVVRRPKDLSARLPSQTADACDIRAGDLFDVSSLGQAAAGCAGAIHLVG
                     IIEENPRAGATFERIHVEGTRNVLQACRSAGVNRYVHMSALGSRPDAPSRYHRTKGRA
                     EELVRESALAWTIFRPSLIHGPEGDFMRMMKSFCTGGLRRPVMPYFGSGNARIQPVSV
                     RDVATCFVAALMKPETVGKTYDLGGPAQYTWREFYDICAEAIVGHRRLKAPVPVFLAK
                     LMARTILPLTPSFLMPYKFNVEQVQMSQEDNVCDIGPVQRDFGLTLRDFRQEVATYAD
                     QIP"
     gene            7549..8238
                     /locus_tag="P5316_09755"
     CDS             7549..8238
                     /locus_tag="P5316_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRR85283.1"
                     /translation="MRVKICGITSVEDALQAVQAGADAIGLNFYAGPRQIDLSTGAAI
                     LDSLPPFVTPVALIGVAGDQIDDDLLTLLIRSRVTLLQVHGDVTIGMMTRLCDVGFAI
                     IQPLAVRDSTFAAVTPFWGEGSWRPTAVILDTYDPNRAGGTGKAFCWDWVEEARRAGQ
                     LDGWPPIILAGGLNPDNVADAIRIARPWAVDVSSGVEPEETPGRKDPGKLRAFIRAAR
                     NAFERNAECDD"
     gene            8226..9272
                     /locus_tag="P5316_09760"
     CDS             8226..9272
                     /locus_tag="P5316_09760"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRR85284.1"
                     /translation="MRRLTISTLAIGLLCAPAPWMSARAAEQAAAEPASFASLADLQS
                     HYAEKIKKTRQAIESERLAALEALLKKAATTERQAILLAMIESAAALEKADQLLLLTD
                     EYLKGSTSIQDAWRVRQVRFMAMVSSDRVDEAKAEWDKASEKMDAVMDVWQQVFDSAI
                     LIADAMLEAGRTTEVSDLYKTLRSKFSFVSNLKEVLQPREAALKWIGKTAPAIEGQDL
                     EGKPVDLTQYKGKVVLIDFWATWCPSCVMTMPELIETYKTHNKSGFEIIGISLDQDRE
                     ALNRYLSSQKIPWRIVFDGKGWFSPSARRYEVSAIPATFLIGRDGKIAMAGTPTKGFG
                     PVVKRLLQSSTDKK"
     gene            9280..10275
                     /locus_tag="P5316_09765"
     CDS             9280..10275
                     /locus_tag="P5316_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85285.1"
                     /translation="MMSVSDFDKSEAVRPERIVAGRRPGGGTMAICCSPLMALCLAGC
                     IGQWRPSNENVSQALQAQRTGRFESCLRANRLGRWLYERIELPEREGRPIRMHVRQVD
                     RERLREGVLEDRPFLKLEEYLDLKRVATRTADEPAATQPAGRDGLTQRRPRAPLDGGT
                     GIFFRLTDAADPMPLELESEKTITSETAVVYYNYRGIPQAKGRLYRCTEFEGIEDIEC
                     PAGRFEGCVRIRVDLRIRFPWVAIVDLTTYLWLSPEVGEVRRVQRLSGWFLVFPFVSA
                     DEYRLVCYGPSAEAAQPPACAAAMWKTGAVLLEGDYPGIRIAGMVIDFEDAQAGQ"
     gene            10340..10957
                     /gene="nadD"
                     /locus_tag="P5316_09770"
     CDS             10340..10957
                     /gene="nadD"
                     /locus_tag="P5316_09770"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRR85286.1"
                     /translation="MARGGLLLFGGSFNPIHVGHLIIARAAAEQLGVERTIVIPSASP
                     PHKTGSDLASPEHRLAMVHLAIADEPAFEVSDIELRREGPSYTLLTVQAYRRQLGADV
                     PLYWLIGGDTLPELHSWYQIAELVDLCRIVTAVRPGFETPDFSTLFGCLSLAQVQRLR
                     EGILDTPRIDISATQIRARVREGRSIRYLVPESVIEYIDRERLYR"
     gene            complement(10985..11311)
                     /locus_tag="P5316_09775"
     CDS             complement(10985..11311)
                     /locus_tag="P5316_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85287.1"
                     /translation="MKRHSKKKPISQLSPAELDALSEEFDREFVVDTFGPLPRSVKNR
                     LDRAKRKPGRPRIGAGSQAISVTIEKKLLQRIDQVARRRKTTRAQLIARGLRTVLQEE
                     QLAAAR"
     gene            complement(11422..11589)
                     /locus_tag="P5316_09780"
     CDS             complement(11422..11589)
                     /locus_tag="P5316_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85288.1"
                     /translation="MEFRWITWNQSKVTSHGVTPEEAEWVIEIAAEPYPQYRKDDKLA
                     CWGPTSSGRLL"
     gene            complement(11665..12636)
                     /locus_tag="P5316_09785"
     CDS             complement(11665..12636)
                     /locus_tag="P5316_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HRR85289.1"
                     /translation="MSLPVVRSVVTVTLNPTIDRIIEVPDFRVGAHLRGRMRSLLPAG
                     KAINVSRAMAALGSPSTAAGWVGAESLALFEDALRQAGVAVRLTPITGATRENITIVD
                     PVGHTETHIRDTGPTVTQDDIQQLTTELTTLSNPDCLLVFTGSCAPGLDASRFVQLLR
                     ACMDGGAHVVVDSSGDHLREAAKLPLWLLKPNLFELGELLGFQISHESEVIEAGRRLN
                     EHIPLVLVTMGERGAMCFAEGRVFKGRAPVPPDSVRSTVGCGDAMLAGFLAATTRPDW
                     QVENAFRQALAVSAASAMTDQPAVFQACDVNQLLQDSEIADVRLGGG"
     gene            complement(12633..13196)
                     /locus_tag="P5316_09790"
     CDS             complement(12633..13196)
                     /locus_tag="P5316_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85290.1"
                     /translation="MSRHQPRNIDRLKLKHSFVLALTIGLLCVGGCDRDQPSPAVPSP
                     DSRPVTAPQATTDIDTTPAVKPPHGGRLLTMPDKQGYIEWVVDAGRLYVLDAAGGPAK
                     AVENVVLTIPSDTGPQQFALIPCGDEAFDGACWTHSAPQLRDEHASAVLRFQFEGGPV
                     RILISEKPPTAPIEDPLPRPTEKLPNQ"
     gene            complement(13310..14719)
                     /locus_tag="P5316_09795"
     CDS             complement(13310..14719)
                     /locus_tag="P5316_09795"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRR85291.1"
                     /translation="MSHRIRIAPRGLAMLAMVGPSLVWCAEYIGSGEVILATRTGALL
                     GTAALWAIVAAIVLKCSIGMAGAQWTAVTGEGMIDLFRRMPGPRHWFIWLVLAVQFPA
                     AIVSIGALAKVAGVFLNSLLPLPGGAITWGAAASVFAVSVSWAGRFDLLKAVMSGLVM
                     IIVVGVLYVAVTVLPPLADIARGLVGLEPMSLPSWAPDAGPNGSPWREILPLMGWAAG
                     GFASQVWYTYWVLGAGYGMAAGRGYGKSADEHRLAILTSEEARTLRGWCRVVRTDAIV
                     AASIGVVITACFCLAGAGVLRPERLVPQGEEVAFTLSRLFSSRWGVLGAKLFLIAGSA
                     AMISTQVGQLAGWPRLLADCIRIICPPFSRFPHVWQFRAFLLFFVLTNLVAVVFFDPV
                     RLVKLGGQLDGILLTPLQALAILAAFFLILPKLVPRHALRELRPGWLLVVLLAIAAVL
                     FGILCFTVLPGSVRSLLTS"
     gene            complement(14880..18887)
                     /locus_tag="P5316_09800"
     CDS             complement(14880..18887)
                     /locus_tag="P5316_09800"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRR85292.1"
                     /translation="MRASPRQVLVSLCAMFCFAIGGPQFLLGAASDDGLWQDAEQHRT
                     ANVQWGSALKSYRLVQLDQTLLMQSLHKAPHESALRADHSPSVITLPMPDGSYQRFSY
                     VESPVMAPELAAQFPEIKTYVGQGIDDPHATVRFDWTPAGFHALIRSPSGSVFVDPSA
                     RGQTTQYVSFYRRDIDPEAAAWTCLTDDHIELSEGLMETEAGPLGAGSGSQLRTYRIA
                     IATTAEYTAYKGGVAQTQSSLATMVNRISGIYEQEFTIRLQLVANNNLLIYTNSATDP
                     YSNGDLSTMINENQTNIDSVIGSANYDIGHVLGTQPSGASGLAGVSVVCSNSKALAGS
                     ACEPWDDESFTIQIIIHEMGHQLGSHHTWNGLGCAADQWGSATACEPGSGTTVMSYAG
                     SCGVDDIQRWNDDYFHAISYEKIRAYCSTGTGGTCGTLTATGNTAPSLTVPGPYTIPK
                     QTPFTLTATASDPNGDPLTYCWEEMDLGPRQALTDPDNGSSPLFRSWPPSPDPSRTFP
                     RLSDLLNNTTPLGEQLPNTNRTLKMRCTVRDNRAGGGGVTNGDTILTVTTSAGPFRVT
                     SPNSAVTWYGSGVVTWDRASTHLAPVNCSYVNIRLSVDGGLTYPYLLASNTANDGSAG
                     VVMPETSSSQARIKVEAVGNVFFDISDTNFSFVCPGPGVATNVSASDGGYPYVYVTWT
                     LPAGALSHCEIWRNTTNNSGTATKIEDNWTTTGYQDASCTPYTTYYYWIKVVNLCGGT
                     SSFSASDSGWRGLAPPANVTASDGDYIDKVRVAWEAAAGATHYRVYRNTTNNSITATA
                     MTSWIPALWWDDTTTSPGALYYYWVQAAMSSTGDHPSDFSAYNTGWRALTPPIVEASK
                     GTSADHVLVQWNSPTGATHYCVYRGVSSDPGQATALTGWITDTSYADTTTPAGVSYYY
                     WVRAAVSSSGLRATGLVVNDVGWKAFTPPANVSATDGTDDTRILITWDASPMATYYRV
                     YRSSTNDPATASAQGSWQTATSYSDYTAYCVDFYYWIKAAATSTGTKASDFSAGDVGW
                     RALPPPTNTSATDGASTNHVQITWNLSPVSGCYYRVYRGSNSNPKMSSPLGDWAYEVT
                     SFDDTTATPGLTYYYWARASMDPQGTRDSVSNSNTGWRALTGPTATASDGTSVDHVAV
                     SWPTVEGASYYRVYRSTTPGSSTAAPVSSWRTNTSYYDTSATPGQTYYYWVKAAVDSS
                     GTHESAFGRGNSGWRAKDCNNNGVPDQNDPDADGDGVPNDCDNCPNTVPGATVDANGC
                     PPLIAPDFNRDGDVDPDDLAVFELCASGPGIPCASGCGSSDFDNDGDVDQADFAVFER
                     CFSGENVPAGAACAN"
     gene            complement(19355..19915)
                     /locus_tag="P5316_09805"
     CDS             complement(19355..19915)
                     /locus_tag="P5316_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85293.1"
                     /translation="MDANELITKIQDKDDKTRAAAVLKAAQAGAGAVKQLAEIMASGE
                     MEVARAAKNALWQIVRHVGRPAADDERKSVVAELLTLCESEQPPAVRREVLWMLSEIA
                     GDETVGAIEPLLANADLREDARMALQRLPGEKSLLALKAALESAPEDFRPNIAVSLRQ
                     RGVDIADYPSAKLVPTKATQVKPVRG"
     gene            complement(19933..>20606)
                     /locus_tag="P5316_09810"
     CDS             complement(19933..>20606)
                     /locus_tag="P5316_09810"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85294.1"
                     /translation="MRWRPYNRKYCPGAFRWIMESGGGQIRDRGAHVMSCAMWWMGAD
                     GTGPVTVEATGTRPTKGLWDAAVEMEVIYTFKNPDWTLIWSQHPDNAPKAEERRPDEP
                     QITRPGYGAVYHGTKGTFTHWGGDGGTWAERKAREWVPPAGAKDVPKSPGHYEDWFEG
                     ITTGRKTIMNIEAAVGVANLCNLGNLAFILGRKIHWDQARQEIVGDEQARRMMGRPQR
                     YPYHL"
BASE COUNT         3733 a         6269 c         6530 g         3974 t
ORIGIN      
        1 gcttctggcc gcctggctgc tggcggattg gcccgccggc cgcttcgttt tccttgacaa
       61 atgggctccg tcgtggcgcc cttggctcag aggcgttgtg gagaaggctc ccgttgggct
      121 gtcgatcatg gccggagcgc tggccctcca tattgtgggt ctgatcgacg accgccgccc
      181 gctgtcagcc cgtgtgaagc tgctcatcca ggcggcggtc acattggcga tcaccgcagg
      241 ggtcggcgtt cgctccgcgg aggcgctcgg accgcttccg gcagtgctgc tcaccacatt
      301 ctggatcatc gcactgacca acgcattcaa tttcatggac aacatggacg gcctgtctgc
      361 cggggtggcg gtgttgacag ccgtcgttct tgcggtttcg tcgatggtcg ccgggcaagt
      421 attcgtcccg tgcgtgctga tgttggtagc agggggtgcg gctggtttcc tgatctacaa
      481 ctttccgccg gcaacgatct tcatgggtga cgccggcagc ctcgtgctgg gttacatgct
      541 ggcggtctgc gcggtgatga ccaccttcta cgaccccgag atgggaacaa cgcccttcgg
      601 cgtgctcgtg cccgtggtgg tgtttgcgat tcccctttac gacatgatca gtgtcattat
      661 ccaccgcctg cggctgggtg tcagcatctt ccgcggcgac agacggcatt tctcgcatcg
      721 cctggttaag cgggggctga gcccgagaac cgccgtcctc acaatctacc tagccacgct
      781 ggcaacgtca cttccggcgg tgttgctgcc cgtgcatggc tgggtggcgg ccctgctgat
      841 cgtcgcccag tgtcttgccg tggtgacgat cattgccatt ctggaatcgt gcaatgaatc
      901 gtgagcccgc tccaaaccgt cggccgccaa ccggagttgc gccggtcgcc ggccgggtgc
      961 cgcatgcgct cgatggtatg gcgttcctgg tgcttctcct gatcgtatcg atgcgcgctc
     1021 tgctttccga aacctacgaa tcaggactga ccggcgtttc gcaggccgtc ggcgacattg
     1081 cgtcgcttac gcccgcgacc acggtgatgc tcgacacggc tatctgggta gcggccgcaa
     1141 tggcggcgtt ggcaaccctg cttcgaagcc gatcgtggca acggaccgga atcgaagccg
     1201 gttgggcaat catggccgtg gccgcagtgt gttcctgctg cgtcgccagc aacaagcgcc
     1261 tggcgatcaa cgcgtcgtgc gactggctaa cgacgccggt cttggcaatc acgctggcga
     1321 acctgctccg cgaaaggaga agagtggtcc tggttctggc tgttgtggtc gcgtcaggat
     1381 tggcttccgc gaccaagtgc ggttcgcaat tgggctggga gttcggcgat acctggcagg
     1441 cctaccagga gcagaaggcc gagttctggg gccggcaggg cgttgctctg accgatccga
     1501 ccgtcgagct gttcgaacgg cgcatgatgg cccgtgaagc aacaggcttc ttgccataca
     1561 gcaacgccca gggcgcgggg ctgatcctgg ccggattcgc cgggattgcc ctggcttttc
     1621 tttccgggcg gtcacgtacg gcgaagggcg tgtgcggggt ggtcgcggcc gttattctcg
     1681 catcggtcgt caccaccgga agccgcggag caatgctggc cgctctcatc ggcctggctc
     1741 ttctgatagc gggcaccctg ataggatcgc gttggtacca gaaatggaag gccctgtggg
     1801 caggcctgtg gataacggct gcgatcggcg ttgccctggt cgtcatctgg gggacgacac
     1861 gcggccgtct gcccggcgac tcgctccgct tcagatggga gtattggcgg gtcacatcaa
     1921 agatcgtcgc tgaacacccc tggacaggcg tcggggcgct caatttcgac cgcgcctacc
     1981 tgaagcacaa accggtccaa tatccggaag agatccgcga tccacacaac ttcgcgatgg
     2041 cgattgtgtc ccagtggggc atcatcggtg gggcggggat gatgattgcc ctgctcggcg
     2101 ggtcctgggt agcgatccgc gccttggggc aggagtcagc atcaccgcct gaggcggcac
     2161 agccgaagga accggtggtc caaccactgg gtaaatgggt agtcgcggtc atcgttggct
     2221 ttgtcctgtt tcggctctgg atgctgcgag gctggctgga cgtcggtgaa ggaggagtgg
     2281 ctacggttct cttcgacctg ttgttgtatg gactcgtctg gtgccttgct ttcgccggct
     2341 tgtcgctgct gttgtcattc gggtgggacg atgccgacat gggcagtgcg tcgacggctc
     2401 cgcaggccaa caagcggcac tggccatccc aaaaggccca tcagcaactt tccccccctt
     2461 accaaggggg gggcaggggg ggtgacggct accggttgcc gtgcctgatt gccctgggcg
     2521 tcttcctcct gcacaacacc atcgacttct ctctctttgt tcccgggaca cttaccactt
     2581 tcgcagcttt gaccgcggtt gccgttgcga gggccgcagg gcagggggaa gggagcgggg
     2641 cggctcggcg ttggctgccg atggttgcgt ccgccggcgg cctgctggcg gtgattatcc
     2701 tgctagtggc gccggtgtta cggtgtacgt cgagtctcaa ggacgcgaga tttgcagtgg
     2761 cgacaggctt gcgcgatccc gcgcccttgt acaaagccgc agccgaggca gatcgcttcg
     2821 atccgacgcc gctgacggaa tgggcctcgt gggcggcaca cttggatgtt actggtttgg
     2881 acaaatcgct gacttgcctg cagatggccc aggatcgcga cccgcttgac cgtgggatct
     2941 accgctcgca gtggcgggtg ttggagctgc gatacgatgc cgcccacaac cccgcggatc
     3001 ttctgcaagc ggtggccgcg gctgagaagg ccgtggacct gtatccgcag tcgccggacg
     3061 ggcacgccga tctggcaaga ttgctgggca gagttgcctc tgagatcgcg tcggatcccg
     3121 ccgcggaagc ggtcggccgt gattggctcg ccgatgccgt catgcactat cgcagggcgc
     3181 tgcatctcga cgccgcccgg ccatcctacg agattcgccg ctggcaccat gaccgcagga
     3241 tgcagatcga gggccgactt tcgcagttgg agcgcatggc ttccgcgcgt gcgggcagtt
     3301 gattccctac gccgcatgtg cagagtgcct ttttcgggtg ttctctcaag ggccgccgac
     3361 ggctcggccg ccaatgtttt cggccacctg ggtctggacg tgtggacaga ctgcctgcaa
     3421 caccctgagt ctgctgtcgc cgaatgggtg gcggacgcct cgttggatcg tgcgtccagg
     3481 ttgtcattgg cgtcgcattt gttagactac tcgcttccgt cggcggcgac gcaatgctcc
     3541 gccgagacgt aacctgcttt gcaacaacga cttggaaagg agtcggcaca atggcggtca
     3601 aaatctacta cgacaaagac gctgatctgg gtgctttgaa gggcaagaaa gtgggcatca
     3661 tgggctacgg cagccagggg cacgcccatg cgcaaaacct gcgagattca ggcatcgagg
     3721 tcgttgtcgc cgaactggcc gacacgccga attacaggtt ggccgtcgaa caccatttca
     3781 aaccggtcag cgtggccgag gccacgaagg cctgtgacct cctggtcatg acgctcccgg
     3841 atgaagtgca agcggccgtt tatcgcaaag agatcgcccc gaatattcgg gccggccagg
     3901 cgctcggctt cacccacggg ttcaacattc acttcggcca gatcgtccca cccgagggcg
     3961 tggacgtgat catgattgcc ccgaagggtc ccgggcatct gctccgcagt gagttcgtca
     4021 agggcggcgg cgtgccctgc ctggtggcgg tcgagaagaa cgcaaccggt aaagctttgc
     4081 agactgctct ggcctggggc tcgggcatcg gcggcggccg ggcgggcatt ctggaaacga
     4141 ccttcaagat cgaaactgag acggatcttt ttggcgagca ggtggtgctc tgcggcggcg
     4201 tgacggctct ggtgaaggca gggtttgaga cgctcgtcga agccggctat ccgccggaga
     4261 tctgcttctt cgagtgcatg cacgagctga aactcatcgt cgacctgttc tatcagggcg
     4321 gcatcaacta catgcgttac agcgtgagca acaccgccga gtacggcgac ctgacccgcg
     4381 gaccgcgaat catcaccgac cagacccgcg cggaaatgaa gaagattctg gccgagatcg
     4441 tcaacggcga cttcgcccgc gagtggctgc tggagtgctc cgtgggacgc ccgagcttca
     4501 atgccctcta caagaaggac tacgatcatc ctctcgaagt cgttggccgg aagctgcgca
     4561 agatgatgaa gtggatcgac tcgaaggaag tctgaccttg acgagtcgag gggtttaccc
     4621 cctcggtctt cttggcgaaa tgtgcctggt tcgccggttt ccgaaagacc ggcggggtaa
     4681 acccgcttga cgagccgagg ggtttatccc ctcggtcttc ttcacgctat ccctgcgttg
     4741 tctcgcgaag accggcggga gaaagcggct tgacgagccg aggggtttac cccctcggtc
     4801 ttcttcacgc tatccctgcg ttgtctcgcg aagaccggcg ggagaaagcg gcttgacgag
     4861 ccgaggggtt taccccctcg gtcttcttca cgctatccct gcgttgtctc gcgaagaccg
     4921 gcgggataaa cccacttgac gagccgaggg gtttaccccc tcggtcttct tcacaccatc
     4981 ccggcggttg tcccgtgaag accggcgggg taaacccgcc ggctcgtcaa gaccaggggt
     5041 ttatcccctc ggttctcttg acgccgttcg tctgccggtc cggcgaagac cggcgggaga
     5101 aagcggcttg acgagccgag gggtttaccc cctcggtnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnact tgacgagccg aggggtttac cccctcggtc ttcttcacac
     5281 catcccggcg gtcgtcccgc gaagaccggc ggggtaaacc cgccggctcg tcaagaccgg
     5341 cggggtaaac ccgccggttc gtcaacaccg atgggaccag cgtccaggcg attcatggca
     5401 gaaccttcat gtcatcaacg ccggccaaga cgaactcctg atcctcgaca tatttgatcg
     5461 cagcctcgat gtggtctgat ccattcaagt accggttgct tccgccacgg gtccaccact
     5521 tgtgtggggc accggcgagg cggttcagcg aagcctgcgt ggtccccttg agaattcggc
     5581 ggacggccgg gccgtcgttg gggcaatccg tgacgactac gtggacgtgg tttgccatga
     5641 tcctcgcact gagcacgcgc catgcccgcg agcgggccgt ttcgacgagg cttgccgcaa
     5701 cgcaggccgc ctgccggcga tccaggtgaa tcgtcttgtg ctgctggcgg cgagatgcct
     5761 gggcgtgggc atacggatcg tccgatctgc acggcgagcc gggtgcgttt tccttgggta
     5821 gccatcgagt cgggttgacc tgcaggttgg acacgtaacc ccgcgagtct ccgggcagcc
     5881 aggttccata gcaggtccag gtgatcagca gtccgaattg cctatcgctt ttcatacacc
     5941 cacgagcata tcccctctgg ccgccactgc aaggaggagc aaacgaagac cggcgggata
     6001 aagccgcttg acgagccgag gggtttaccc cctcggttct cttgacgccg ttcgtttgcc
     6061 ggtcccgcga agaccggcgg ggtaaacccg ccggctcgtc aagaccaggg gtttatcccc
     6121 tcggttttct tcacactgtt cctccgtcgt cccgcgaaga ccggcggggt aaacccgctt
     6181 gacgagccga ggggtttatc ccctcggttc tcttgacgcc gttcgtttgc tggtccggcg
     6241 aagaccggcg gggtaaaccc gccggctcgt caagaccagg ggtttatccc ctcggttttc
     6301 ttcacactgt tcctgcgtcg tcccgcgaag accggcggca taaagccgcc ggctcgtcaa
     6361 gtcagggtat ctgatcagcg taggtcgcta cttcctgacg gaaatcacga agtgtcaggc
     6421 cgaaatcccg ctgtaccggt ccaatatcgc agacattatc ctcttggctc atctgcacct
     6481 gctccacatt gaacttgtag ggcatcagga aggaaggcgt caggggcaag atggtccgtg
     6541 ccatcagctt tgcgagaaaa acaggaacag gagccttcaa ccgtcgatgc ccgacgatcg
     6601 cctcggcgca gatgtcgtag aactcgcgcc aggtgtactg agccggcccg ccaaggtcgt
     6661 aggttttgcc gacggtctcg ggtttcatca gggcggcgac aaagcacgtc gccacatcgc
     6721 gaacactcac cggctgaatg cgagcgttgc cgctcccgaa atagggcatc acaggccggc
     6781 ggagcccgcc cgtgcagaag gatttcatca tccgcatgaa atcgccttcc gggccgtgga
     6841 tcaggctggg gcgaaagatg gtccacgcca acgcgctttc gcgaacaagc tcctcggccc
     6901 gccccttggt ccggtggtat cgactcggcg catccggccg actgcccagc gccgacatgt
     6961 ggacataacg attgactccg gccgatcggc aggcctgcag cacattccgc gttccctcaa
     7021 cgtgaatccg ctcgaaggtg gcaccggcgc ggggattctc ctcaatgatc ccgaccagat
     7081 gaatcgcgcc tgcgcagccg gccgcggcct ggccaagcga ggagacatca aacagatcgc
     7141 ccgcccgaat atcgcacgcg tccgcagtct gcgacggcag gcgcgccgac aagtccttcg
     7201 gccgtcggac aacacacacc ggccgatacc cttgatcgac cagctctcgg agcacgtacc
     7261 ctccgacgaa gccggacgca ccggtgagga gaactcgccc gagcccaccg gcctcgcggc
     7321 caggctgtgt cgcgctggtg ctcggcatcg aatcgggatc ctgagagtcc gacatgggtc
     7381 cttcctcctc cccgcagtct atccgtgaga tcataccaag aaaaggcgaa tccgccgccc
     7441 gctccgggcg atacgatcgt ccgaacagtt cgcccggcgt tgaccgcctg acatgatagc
     7501 cctaatattc cggccgccgg ccgcatagct gggggacgga atcgaaacgt gcgagtcaag
     7561 atctgcggca ttacgagtgt cgaggatgcg cttcaggccg tccaagccgg cgccgacgcc
     7621 atcggtctga acttctatgc cggcccccga cagatcgacc tgtctacggg ggcggccatc
     7681 ctggattccc tgccgccttt cgtgacgccc gttgctctga taggggtcgc aggcgatcag
     7741 atcgacgatg atctcctgac cttgctcatt cgcagccgag tgaccctcct ccaggttcac
     7801 ggtgatgtca cgatcggcat gatgacccgc ctttgcgacg tcggctttgc gatcatccaa
     7861 ccgctggcgg tacgtgattc gacgtttgcg gctgtgacac cgttttgggg cgagggatcg
     7921 tggcgcccga cggccgtgat ccttgatact tacgatccta accgtgccgg cgggaccgga
     7981 aaggcctttt gttgggactg ggtggaggag gctcgcaggg ccgggcaatt ggacggatgg
     8041 ccacctatca tcttggcagg cggcttgaat ccggataatg tagccgatgc gatacgaatc
     8101 gcccggcctt gggcggtgga cgtgtcttcc ggtgtggagc cggaagaaac gccggggcga
     8161 aaagaccccg gcaagctgcg ggctttcatc cgcgccgcac gaaacgcctt tgaaaggaac
     8221 gctgaatgcg acgactgact atttcgactc tcgcgatcgg gctcctgtgc gccccggcgc
     8281 catggatgtc ggcacgtgcg gccgagcaag cggcggcgga acctgcgagt ttcgcctctt
     8341 tggcggactt gcagagtcac tacgcggaga agatcaagaa gacccgccaa gcgatcgagt
     8401 ccgagcgtct tgcggccctc gaggctctgc tgaaaaaggc tgccaccacc gagaggcagg
     8461 caatcctttt agccatgatc gagtcggccg cagctctcga aaaggccgac cagttgctct
     8521 tgctcactga tgaatatctg aagggatcga ccagtattca ggatgcgtgg cgtgtccggc
     8581 aggtcaggtt catggcaatg gtttcgtccg accgcgtgga cgaggcaaag gcggaatggg
     8641 acaaagccag tgaaaagatg gacgcggtca tggacgtctg gcagcaggtg ttcgactcgg
     8701 cgattctgat tgccgacgcg atgttggaag ccggccgtac cacggaggtc tccgacctgt
     8761 acaagacgct tcgcagcaag ttttccttcg tctccaatct caaagaggtt ctgcaaccgc
     8821 gcgaagcggc cctcaagtgg atcggcaaga cggcgccggc cattgaaggg caggacctgg
     8881 aaggcaagcc ggtcgatctg acgcaataca agggcaaggt cgttctgatc gatttctggg
     8941 cgacatggtg cccatcgtgt gtcatgacca tgcccgagct gatcgagacg tacaagacgc
     9001 ataacaagtc gggcttcgag atcatcggga tcagtctgga tcaggacagg gaagcgctga
     9061 accgctatct cagctctcag aagataccct ggcgaatcgt ttttgacgga aagggctggt
     9121 tcagcccaag cgcacgaagg tacgaggtca gcgccattcc cgcaacgttc ttgatcggtc
     9181 gcgacggcaa gattgcgatg gccggtacac cgacgaaggg tttcggtccc gtggtcaagc
     9241 ggctgctgca gtcctcgacg gacaagaaat aaccctccca tgatgtcggt gtctgacttt
     9301 gacaaatcag aggcggtccg cccggaacgc attgtcgcgg gacgacgtcc cggcggcggg
     9361 acaatggcca tctgctgcag cccgttgatg gcgctttgcc tggcaggatg catcgggcaa
     9421 tggcgtccgt cgaacgagaa cgtttctcaa gcactccagg cgcagcgtac cggcagattt
     9481 gagtcttgcc tgcgtgccaa ccgccttggc cggtggctct acgagcgaat cgaactgccc
     9541 gagcgcgagg gccggccgat ccgaatgcat gtcaggcagg ttgaccggga acgattgcgg
     9601 gaaggagttc ttgaagaccg accgttcctg aagctcgagg agtacctgga cctcaaacgg
     9661 gtcgccacgc gaacggccga tgagccggcg gcgacgcagc ccgccggtcg tgacggcttg
     9721 actcagcgaa gaccgcgagc cccccttgac gggggcacgg gcatcttctt tcgtttgacc
     9781 gacgccgccg atccgatgcc gttggagttg gaatcggaga aaacgatcac cagcgagact
     9841 gcggtagtct actacaatta ccggggcatt ccgcaggcga agggcaggct ctatcgatgc
     9901 accgagttcg aaggcatcga ggacatcgag tgtccggccg ggcgttttga ggggtgcgtt
     9961 cggattcggg tggacctgag aatacgcttc ccgtgggtgg ccatcgttga tctgacaact
    10021 tacctctggc tctcacccga ggtcggcgaa gtccgacgcg tgcagcggct cagcggctgg
    10081 tttctggttt ttccatttgt cagtgcggac gagtatcggc ttgtttgtta cggtccgagc
    10141 gcggaagccg cgcagccgcc ggcctgcgct gcggcgatgt ggaagaccgg ggccgttctt
    10201 ctggagggcg attacccggg catccgcatc gccggcatgg tgatcgattt cgaggatgcc
    10261 caggcagggc aatgacgggc atggctggct gcacggcggc cgcgcgttta tattcacggc
    10321 cgtgatcgag ggctggacaa tggcacgcgg cggcttgctg ctcttcggcg gaagcttcaa
    10381 tcccatacac gtcgggcacc tgattattgc gcgcgccgcc gccgagcaac tgggcgtcga
    10441 gcggacgatc gtgatcccct cggcctctcc accacacaag acgggcagcg atctggcatc
    10501 tcctgagcac cgcctggcca tggtgcatct ggccatcgcc gatgagccgg ccttcgaggt
    10561 cagcgatatc gagcttcgac gcgagggacc gagctacact cttctgacgg tccaggcgta
    10621 tcgccgacag cttggtgccg atgtgcccct gtactggctg atcggcggcg acacgctgcc
    10681 ggaactgcac agttggtacc aaatcgccga actcgtcgat ctctgccgca tcgtcactgc
    10741 cgtgcgccca ggctttgaaa cgcccgactt ttcgacgctg ttcggctgcc tgtcgctcgc
    10801 ccaggtccag cggctgcgcg aagggattct cgatacgccg cggatcgaca tctccgccac
    10861 acaaatacgc gcgcgagtcc gcgaggggcg atcgattcgc tacctggtgc cggaaagcgt
    10921 tatcgagtac atcgaccggg agcgactcta tcgttaaagg tcgggtcagc gtttgtcgtc
    10981 ggggtcaacg agcggcggcg agttgttctt cctgcaaaac ggtacgcaag ccgcgagcaa
    11041 tgagttgggc gcgggtcgtc ttgcgacggc gggcgacctg gtcgattctc tgcagcagct
    11101 tcttctcgat ggtcacggaa atcgcttgcg acccggctcc gatccgcggg cggccgggct
    11161 tgcgtttggc tcggtccagg cgattcttca ccgagcgagg caacggaccg aaagtgtcca
    11221 cgacgaattc ccggtcaaac tcctcggaca acgcgtccaa ctccgctggg ctgagctggg
    11281 agatcggttt cttctttgag tgtcttttca tcgcttacct cttcgtcgtc gttggcggtt
    11341 ccagcggcgc ttctcgcggg ttgtcagcgg gcgcgcgtga ataaccaacg cgctgtcatc
    11401 ctcgtcgagg acgtagacaa cctacagcag ccttccgctt gatgtcggcc cccagcaggc
    11461 cagtttgtca tccttccgat actgtgggta cggttcggcg gcaatctcaa tcacccattc
    11521 cgcttcctcg ggcgtgacgc cgtggcttgt caccttcgac tgattccacg ttatccatcg
    11581 gaactccacg tcatgccctc taatattata tatacaaaac tcggcacggc caactcatac
    11641 cgcagaaccc cgaattcagg tcgatcatcc gcctccgagc ctaacatcag cgatttcact
    11701 gtcttgcaac aactggttga cgtcgcaggc ctggaagacc gcgggctgat cggtcatggc
    11761 cgatgccgcc gagacggcca gggcctggcg gaaggcgttc tcgacctgcc aatccggacg
    11821 cgtggtcgcg gcgagaaagc cggccagcat ggcatcgcca cagccgaccg tgctacggac
    11881 gctatcgggc ggaaccggcg cccggccttt gaagacccgt ccttcggcaa agcacatcgc
    11941 ccctcgttca cccattgtga ccaggacgag cggtatgtgc tcgttgagcc ggcggccggc
    12001 ctcgattact tcgctttcgt gagaaatctg aaagccgagc aattcgccga gttcgaaaag
    12061 gttcggcttg agcagccaca aaggcagctt tgccgcttca cgaagatgat cgccggaact
    12121 gtccaccacg acgtgagcac ctccgtccat gcacgcccgc agtagctgaa caaaccggct
    12181 ggcgtccagg ccgggggcac acgatcccgt gaaaacgaga aggcagtcgg gattggagag
    12241 ggtggtcagc tcggttgtca gttgctgaat atcatcttgg gtcaccgtcg gtccggtgtc
    12301 gcgaatgtgg gtttcggtgt gcccgaccgg atcgacgatc gtgatgttct cgcgggtggc
    12361 tccggtgatc ggagtcaggc ggacggccac gcccgcttga cgcagggcgt cctcgaacaa
    12421 cgctaacgat tcggctccca cccaaccggc cgcggtgctc ggcgacccga gggcggccat
    12481 ggctcgcgag acgttgattg cctttccggc cggcagcagc gaccgcatgc gacccctcag
    12541 gtgcgcgcca acacggaaat cggggacttc gataatccga tcgatggtcg ggttcaacgt
    12601 gacggtcacg acggatcgaa cgacgggcag actcattgat tcggcagctt ttccgtgggc
    12661 cggggcagag gatcctcgat tggggccgtc ggcggtttct cgctaatcag aatgcgcacc
    12721 ggtccgccct cgaactggaa tcgaagcacc gctgaggcgt gctcatcacg cagttgcggc
    12781 gcagaatgcg tccagcacgc accatcgaac gcctcatctc cacagggtat caaggcaaac
    12841 tgctgcggtc cggtgtccga cgggatggtc agcacgacgt tttcaactgc cttagccggg
    12901 ccgcccgcag cgtccagcac ataaagccgt ccggcgtcga cgacccactc gatgtacccc
    12961 tgcttgtccg gcatggtcag gagacggccg ccatgagggg gcttcacggc cggagtcgtg
    13021 tcaatgtcgg tagtggcctg cggcgcagta acgggtcggc tgtccggtga cggcaccgca
    13081 ggcgacggtt ggtccctatc gcagccaccc acgcacaaca ggccgatggt caacgcaagg
    13141 acaaacgagt gcttgagttt caatcgatcg atgttgcgag gttgatgcct gctcatgtta
    13201 ctacaacttc ctgcttgtca gaatcgtgcc tggcgatgtg tcccaggcgc ctcggcgtgc
    13261 aggtattcta gtgggtgcgc cggcaggcgc caactgaccg gtaccggcct cacgacgtga
    13321 gaagcgaccg gacactgccg ggcaacacgg tgaaacacag gattccgaag aggactgccg
    13381 cgatggcaag caacacgacc aacagccagc cgggacgaag ctcacggagg gcgtggcgcg
    13441 gaacgagctt gggcagaatc aggaagaacg ccgcaagaat cgccaacgct tgtagcggcg
    13501 tgagaaggat gccgtcaagc tgaccgccga gcttaacaag cctgaccggg tcaaagaaga
    13561 cgactgcaac gaggttggtg agcacaaaga agagcaagaa ggcccggaat tgccagacgt
    13621 gcgggaagcg tgaaaagggc gggcagatga ttcggatgca atcggcaagc agccgcggcc
    13681 agcctgcaag ctgaccgacc tgggtgctga tcatggctgc cgagccggcg atcaggaaca
    13741 acttggcgcc cagcacaccc catcggcttg agaagagacg ggagagagtg aaggcgacct
    13801 cctctccctg gggaaccaga cgctcgggcc gcaagacgcc cgccccggcc aggcaaaagc
    13861 aggcggtgat gacgacgccg atggacgctg ccacgattgc atcggtccgg accacacgac
    13921 accagcctcg caaggtgcgg gcctcttcgg acgtcaggat cgccaggcgg tgctcgtcgg
    13981 cgctcttgcc gtatccgcgt cccgcggcca tgccgtagcc ggcgccaaga acccaatagg
    14041 tataccagac ctggctggca aatccgccgg ctgcccagcc catgagcggg agaatctctc
    14101 gccacggtga accgtttggg ccggcatcgg gtgcccaaga cggcaaggac atgggttcga
    14161 gtccgaccaa tccgcgggcg atgtcggcca gcggcggcag cactgtcaca gcgacgtaga
    14221 gcacgccaac gacgatgatc atcaccaggc cgctcatcac cgccttgagc agatcgaaac
    14281 gcccggccca tgagacgctg accgcaaaga cggaggcggc cgctccccag gtgatcgctc
    14341 cgccgggcaa gggcagcagc gagttcagaa agacgccggc gaccttggcc agcgcgccga
    14401 tggagacgat tgcagcgggg aactgcacgg ccaggacgag ccagatgaac cagtgccgcg
    14461 ggcccggcat ccgccggaag aggtcgatca ttccctcgcc agtaaccgcc gtccactgcg
    14521 caccggccat tccgatgctg cacttcagga cgatggccgc gacgatcgcc cacaatgcgg
    14581 ccgtgcccag cagcgccccg gtgcgcgtgg cgagaatcac ctcgcctgag ccgatgtact
    14641 cggcgcacca gacgagcgat gggccgacca tggccagcat ggccaagccg cgcggtgcaa
    14701 tgcggattct gtgagacatg ccgtgtcgcc tctcgaactg acgggcgaat tgtacggcac
    14761 ggcaccgatt atgaacaggc tgtccgcaac tccggcgaga gactcagagt ctgacagggc
    14821 acggccgcgc acgagcggac aagccgccag tgcaatggct gggctgacca aactgcacat
    14881 cagttcgcgc aagccgcgcc ggccggcacg ttctcgccgc tgaagcaccg ttcgaagacg
    14941 gcgaaatcag cctggtcaac gtcgccgtcg ttgtcaaaat cactgctgcc acagccgctg
    15001 gcgcagggga tgcccgggcc ggacgcacag agttcgaaga cggcgaggtc gtccggatcg
    15061 acatcgccgt cgcggttgaa gtccggggca atcagcggcg ggcaaccgtt tgcgtcgacg
    15121 gtcgcgccgg ggaccgtgtt cgggcagttg tcacagtcgt tgggtacccc gtcgccatca
    15181 gcatcgggat cgttttgatc ggggaccccg ttgttgttac agtcctttgc ccgccatccg
    15241 gagttgcccc ggccgaacgc gctctcgtgg gtgccgctcg aatcgacggc cgccttgacc
    15301 cagtaatagt aggtttggcc gggggtcgcc gacgtgtcgt agtagctcgt gttcgtccgc
    15361 cagctcgata ctggcgcagc cgtgcttgag ccgggcgtcg tgctgcgata gacgcggtaa
    15421 taggaagcgc cctcgaccgt cggccacgaa acggcgacgt gatccacgga ggttccgtca
    15481 ctggccgtcg cggtcgggcc ggtcaatgcc cgccagccgg tgttcgaatt gctgaccgaa
    15541 tcgcgcgtcc cctgcggatc catagaggcc ctggcccaat agtaatatgt caggcccggc
    15601 gtcgcggtcg tgtcgtcgaa cgaggtcact tcatacgccc agtcccccag gggcgaagac
    15661 atcttcggat tggaattgct gccccgatag acacgatagt agcaaccgga tacggggctg
    15721 agattccacg tgatctgcac gtggttggtg gacgcgccat cggtcgcgct ggtgttcgtc
    15781 ggcggcggca gggcccgcca gccgacatcc ccggcgctga aatcgctggc cttggtgccc
    15841 gtggaagtcg cggcggcttt gatccagtaa tagaaatcca cgcagtacgc agtgtaatcg
    15901 ctgtagctcg tggcggtctg ccaggagccc tgtgccgatg cggtggccgg gtcgttcgtc
    15961 gagctgcggt agacgcgata gtacgtcgcc atcggcgatg cgtcccacgt gatcagaatg
    16021 cgggtgtcgt ccgtaccgtc ggtcgcggac acgttggccg gcggcgtgaa cgccttccac
    16081 ccaacgtcat tgacgaccaa gccggttgcc cgtaagccgg agctggagac agccgctctt
    16141 acccagtagt agtaggacac tcccgccggc gtcgtcgtgt cggcgtagct ggtgtcggtg
    16201 atccatcccg tcagcgctgt ggcctggccg gggtcgctgg acacaccgcg atacacgcaa
    16261 tagtgggtcg cgcccgtcgg gctgttccat tgcacgagaa cgtggtcggc cgaagtcccc
    16321 ttggaagcct cgacaatcgg aggggtgagt gcccgccagc cggtgttgta cgcgctgaag
    16381 tcgctcggat gatcgccagt actgctcatg gcagcctgaa cccagtagta atatagcgcg
    16441 ccgggggagg ttgtggtgtc gtcccaccac agcgcgggaa tccacgaggt catggccgtg
    16501 gccgtgatgc tgttgttggt cgtgttgcga tagacgcggt aatgcgtcgc cccggcagca
    16561 gcctcccaag cgacccgcac cttatcgatg taatcaccgt cggacgccgt cacattcgcc
    16621 ggcggcgcca gcccccgcca gccgctgtca gacgcgctga agctgctcgt cccgccgcag
    16681 agattgacga ccttgatcca gtagtagtaa gtcgtatatg gggtgcagga ggcgtcctgg
    16741 taaccggtcg tggtccagtt atcctcgatc ttggtggctg tgccgctgtt gttcgtggtg
    16801 ttgcgccaga tctcgcagtg cgacaaggcg cccgccggca acgtccaggt gacatagacg
    16861 taggggtagc cgccgtcaga ggcagagacg ttcgtcgcaa cgcccggccc cggacataca
    16921 aaactgaagt tggtgtcgct gatgtcaaag aagacgtttc cgacggcttc caccttgatg
    16981 cgtgcctggc tgctggatgt ttccggcatg accacgcccg cagagccgtc attggcggtg
    17041 ttgctggcca ggaggtacgg atacgtgaga ccgccgtcga ccgagaggcg aatgttgacg
    17101 tagctgcagt tgacaggcgc caagtgagtc gatgccctgt cccatgtcac taccccggaa
    17161 ccataccaag tgaccgcgga gttcggggag gtcacccgga acggtccggc tgacgtggtc
    17221 accgtgagaa tggtatcgcc attggtcacg cctccgcctc cggcgcggtt atcgcggact
    17281 gtgcagcgca ttttcaatgt gcggttcgta ttgggaagtt gctcgccgag gggtgtcgtg
    17341 ttgttcagca aatcgctgag ccgcgggaag gtccgagagg ggtcgggtga gggcggccag
    17401 gagcggaaga gcggactgct gccgttatcc gggtcggtca gggcctgccg gggccccaag
    17461 tccatttctt cccagcagta agtcagcgga tcaccgttgg gatcgctggc ggtggccgtc
    17521 agggtgaacg gcgtctgctt gggaatggta tacggtccgg gcacggtgag tgagggggcg
    17581 gtattgcctg tcgcagtgag ggtgccacac gtcccgcccg tgccggtcga gcagtaagca
    17641 cggattttct catagctgat tgcgtggaag taatcatcgt tccatcgctg gatgtcatcc
    17701 accccgcagg aaccggcata ggacatgacc gtcgttccgc tgcccggctc acacgccgtc
    17761 gcactgcccc attggtcggc ggcgcagcca agaccgttcc aggtgtggtg tgacccgagt
    17821 tgatgaccca tctcatgaat gatgatctga atggtgaacg actcgtcatc ccacggctcg
    17881 caggcggagc ccgccaacgc cttgctattg ctgcaaacca cgctgacgcc cgccaggccg
    17941 ctggcaccac tgggctgcgt ccccaggacg tggccgatat catagttggc cgagccgatg
    18001 acgctgtcga tattggtctg gttctcattg atcatcgtgc tcaagtcgcc attgctgtaa
    18061 gggtcggtcg cgctgttggt gtaaatcagc aggttgttgt tggccaccag ttgaagacgg
    18121 atcgtgaact cctgctcata gatcccgctg atgcgattga ccatagtggc gagcgaagat
    18181 tgggtctgtg cgacaccacc cttgtaggct gtgtactcgg cggtggtggc aatcgcgatg
    18241 cgatacgttc ggagctggct gcccgaaccg gcgcccaacg ggccggcctc ggtttccatc
    18301 aacccttcgg aaagctctat gtggtcgtcg gtgaggcagg tccaagccgc ggcttccgga
    18361 tcgatgtcgc ggcgatagaa actgacgtat tgtgtcgtct gtcccctggc ggacggatcc
    18421 acgaataccg aacccgaggg ggaacgaatc agcgcatgga acccggccgg cgtccagtcg
    18481 aatcgcaccg tcgcatgagg atcatcgata ccttggccaa cataagtctt gatttccgga
    18541 aactgggccg ccaattccgg ggccatcacc ggcgactcga cgtagctgaa ccgctggtag
    18601 cttccgtcgg gcatgggcag ggtgatcacg gacggcgagt ggtccgcgcg cagggcggac
    18661 tcatgcggtg ctttatgcag gctttgcatc aaaagcgtct gatcaagctg aaccagccga
    18721 tatgacttca gggcgctgcc ccattggacg ttggcggtgc gatgctgttc ggcatcctgc
    18781 caaaggccgt catccgaagc tgctccgagg agaaattgag gacccccaat ggcgaagcag
    18841 aacatcgcgc acagggacac caagacctgc cggggtgagg ctcgcatact ataagctcct
    18901 ttcagctacc gattgccgac gttttcgtaa cgggatcggg gagcggatat gcgggccatt
    18961 caccggcctc agcggacacg tctcgccgaa acgaaacggc caagcgacgg ccactggctc
    19021 atggcaacca tacacacccc ctgggccacg atatgctgat tctacccaga tctaacgaca
    19081 aacgccagct ttgcgaaacg atcagtgtag ccgtccaagg tgcagtatga ccacacgaga
    19141 agcggttgct ttggcattcc ccttcgcaaa gcgtacagtt caagcgtgaa cgcgtggtcc
    19201 gattaccacg attccgacat cctgacaggc aaacgcggta ccggtagccg tgacggccga
    19261 gacttaccgc cggcatcttc gcatcgacgg cacgaccggg cttgcgctgc caagcccttg
    19321 tattcgttgc gcgccgaatc ctcggtctcg cccctcatcc tctgactggt ttgacttggg
    19381 tggccttcgt gggaacgagc ttggcgcttg gataatcggc gatgtcgacg ccacgctgac
    19441 gcagcgatac ggcgatattc ggcctgaagt cttccggcgc ggattccaag gccgctttca
    19501 gtgccagcag ggacttttcg cctggcagac gctggagtgc catgcgggcg tcttctcgca
    19561 agtccgcatt ggccaacagc ggctcgatcg caccgacggt ctcgtcaccc gcgatttccg
    19621 agagcatcca caagacctcg cgccggacgg ctgggggctg ctcgctttcg cacagggtca
    19681 acaattcggc caccaccgac ttacgctcgt cgtcggcagc aggtcgccct acatgacgaa
    19741 cgatctgcca taaggcgttt ttggcggctc gggccacctc catttcgccg ctcgccatga
    19801 tctcggccag ttgcttgacg gcgccggctc cggcctgcgc ggccttgagc acggcggcag
    19861 cccgcgtttt atcatctttg tcctggatct ttgtgatcag ttcgtttgca tccatgttgg
    19921 catttccatc tttcaaaggt ggtaaggata ccgttgtggc cgtcccatca tgcgcctggc
    19981 ctgctcatcg ccgacgattt cctgccgagc ttggtcccag tgaatcttcc ggccgaggat
    20041 aaaggccaga ttcccgagat tgcacagatt ggccacgccg acggccgcct cgatattcat
    20101 gatggttttc ctgccggtcg tgatgccttc gaaccagtct tcgtagtggc cagggctctt
    20161 ggggacgtcc ttggcaccgg caggcgggac ccattcgcgg gcctttcgct ccgcccaagt
    20221 acccccgtca ccaccccagt gggtgaaggt gcctttggtg ccgtgataca ccgcgccata
    20281 gccgggccgc gtgatttgag gttcatccgg gcgtcgttct tcagccttgg gggcgttgtc
    20341 cggatgctgg ctccagatca atgtccagtc agggttcttg aacgtgtaga tgacctccat
    20401 ttcgacagcg gcgtcccaca agcccttggt tggccgcgtg ccggtcgctt cgaccgtcac
    20461 cgggccggtg ccatcagctc ccatccacca catggcgcag ctcatgacgt gggccccgcg
    20521 gtcgcggatt tggccgccgc ccgattccat gatccagcgg aacgcgcccg ggcagtattt
    20581 ccggttatat ggtcgccagc gcatcg
//
LOCUS       DAOTPW010000089        20585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2665_length_20585_cov_5.429664, whole genome shotgun sequence.
ACCESSION   DAOTPW010000089 DAOTPW010000000
VERSION     DAOTPW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20585
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2665_length_20585_cov_5.429664"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="P5316_09815"
     CDS             complement(<1..1500)
                     /locus_tag="P5316_09815"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRR85295.1"
                     /translation="MSTSQEESGDGQSMIRIFDTTLRDGEQSPGASLNTAQKLEIARA
                     LERLNVDIIEAGFPITSPDDFEAVQAVSGIVRGCSVAGLARCTEKDIVRAAEAVKEAA
                     RPRIHVFCATSAIHRQYKLKRAEAEIIKLSVEGVKLAKSFVEDVEFSPEDASRTELPF
                     LAEVVEATIEAGATTVNIPDTVGYSTPDHFTNIIEYLMTHVKSIKKAVISVHCHNDLG
                     LAVANSLAAVKAGARQVECTINGLGERAGNASLEEVVMAIKTRADVYKARTRINTKMI
                     YPTSRLVASLTGIHVQRNKAIVGQNAFAHEAGIHQHGVLAHRETYEIMNPQDVGIPES
                     KLVLGKHSGRHALRDRLLQLGIELDDKQLDMVFEQFKTLADRKKEVFDEDIEAIVGQL
                     FEEAEAMRTWELVSLQTMGGTGVTPTATVQLRSLADDKTYLDAATGDGPIDAAFSCIT
                     RLTGVEAKLQEFDLRAVTGGKEAQGEANLELVCNGKTYRGRGRSTDIIEA"
     gene            complement(1955..4150)
                     /locus_tag="P5316_09820"
     CDS             complement(1955..4150)
                     /locus_tag="P5316_09820"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HRR85296.1"
                     /translation="MRRYSLLIAIGFLFSLSGANCLDFLNNKPRVAGLKGFSSGEELR
                     SYFASQASNSMRNNYRNPFPIFWGGFPMAPTDMATGGDAANGAEGGGADRGDDYSGTN
                     IQEAGVDESDLVKNDGDYIYVLDESTVHIASVSPLAEAASIKLDSYGDSLYLYGNKLI
                     ALSQEYTYYPMPLLMDSSVRFGAARESLAETGEGSEEPPGEGSDASEGEGDCTTSGDD
                     SGASCDDGDDSSDDLDEDDIVGGPWNDGSQVLVTIIDVTDPANPAVEAKIRLEGSLAS
                     SRMIESKLHVVLTTTPRLPYDITFRELDEITLEEWLPDYEIENASGGKQSGDIVGWEN
                     VFRPLDEQGYGLTTVVTLDVNDPTGEPVSTSIVADAGTIYASTEALYVTDTEYGWSGF
                     WEITRTDTEIHKFRFTETGTEYAASGLVPGRPLNQYSLGEYQDYLRIATTEDDFSGNG
                     NGTTNNVYVLGQQGDALNIVGSVENIGRGETIYSARFIGERGFVVTFRRTDPLFVIDL
                     SEPTSPQMKGELICPGYSDHIQLLDENHLLTIGKDAEPDGDWAWVKGVQLSVFDVSDM
                     EHPQLQYRERIGGRGTNSEANYNPKAFNLWKPQGEDYGLLAFPIDVYDEGTSGNPWSY
                     GDWKFSGLMVYRVSVANGFEYLGGISSVDTSQVNGCYWGYYGGYTRGVFIRDSVFSVT
                     PNGVKAAAIGEVSTITAEVEFAGADPPYDYCGGWVEPMIMVPDEAVDLR"
     gene            4693..5646
                     /locus_tag="P5316_09825"
     CDS             4693..5646
                     /locus_tag="P5316_09825"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HRR85297.1"
                     /translation="MFGFLKSLFSRRVEPALPPPRFTTVDLARRLGVDAAELARVDVS
                     YKAFQVPKRSGGMRTIQAPAPPLMAVQRLILRRLLRRLRAHPNATGFERGHSIVTNAL
                     PHVDKDIVIRLDLVNFFPSISADRVERYFRFIGYDQEAATILTRLCTYKGSLPQGAPT
                     SPRLSNLVNYRLDARLAALAESRGLSYSRYADDITFSARIADLAATRCANPKALERQK
                     RRQPRPNDLIHAAKAVIASEGYRLHMHRKLRVSRRHDRQLVTGLVVNSKPDLPRATRR
                     RLRAVADHLRRGRPATLTPQQLAGWHALQAMIAAQRATPAS"
     gene            5741..6211
                     /locus_tag="P5316_09830"
     CDS             5741..6211
                     /locus_tag="P5316_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85298.1"
                     /translation="MRRTTVIGLACAVTSSVVALAQDAPASRPGDVPQCEIVSVKRIW
                     DAAPHNAFTDLIRFQDKWYCTFREGEGHVGGDGKLRVLESVDGDKWSSAALLTEEGVD
                     LRDPKLSITADGRLMMVAGGSIYKGKELVGRPGTNLVNGHLVNNDLLRSVAPAL"
     gene            complement(6310..7392)
                     /locus_tag="P5316_09835"
     CDS             complement(6310..7392)
                     /locus_tag="P5316_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRR85299.1"
                     /translation="MVSINDSRLFSGAFQGCILGTAVGDAIGLPREGLSARRAARLFG
                     SAPLDHRLLIGRGMVSDDTEHTCMVAQALLASKGNPDAFARSLAWKLRWWLLGLPAGV
                     GSATARSILKLWLGFPPHRSGVWSAGNGPAMRSAILGVYAGDDDDLLLRLVHASTVIT
                     HCDPAAEHGALAVALAARYGAMRGSEGVRPAEFLAHAEKYLKDAPLMHLLSRANDHLQ
                     QGDDAAEYAVALGLRHGVSGYINHTVPVALYCWLRRPDDFRSAVEAAVLLGGDTDTTG
                     AIVGALMGATLGVEAIPAEWLDGLAEWPRTTQWMRTLGEHLSEAKTHAGMEGQLPLFW
                     PDVLLRNVFFLGVVLAHVLRRCLPPY"
     gene            complement(7411..7962)
                     /locus_tag="P5316_09840"
     CDS             complement(7411..7962)
                     /locus_tag="P5316_09840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HRR85300.1"
                     /translation="MNSEEKKRTSKFLSYVLRHRPDEIGLELDRAGWVSVDVLLVQCE
                     AHGRPISWEQLEEVVLTNDKQRFEFSDDQAMIRARQGHSIKVDLGYVPVEPPEVLYHG
                     TAVHNLESIRDKGLLKGKRHHVHLSAETVMTLKVASRYGKPVLLKIDARKMHQDGHVF
                     YRTGNNVWLTDHVPPEYLTWPET"
     gene            complement(7959..8648)
                     /locus_tag="P5316_09845"
     CDS             complement(7959..8648)
                     /locus_tag="P5316_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRR85301.1"
                     /translation="MPHTYQYPRPSLTVDCVVFGLDDEDLKVLLIQRDLPPFEGQWAL
                     PGGFVRMDETLEEAAERELREETGLSDVYLEQLYTFGDLGRDPRERVVTVAYYALVNL
                     RDHRVQAATDARNAAWFAVEDCAKLAFDHARILRMALQRLRGKVRYQPIGFELLPPKF
                     TLRQLQHLYEKVLDRPLDKRNFRKKILGMGILIELDEVETDVAHRAARLYRFDKKHYD
                     RLTKKGFNFEI"
     gene            complement(8760..10232)
                     /locus_tag="P5316_09850"
     CDS             complement(8760..10232)
                     /locus_tag="P5316_09850"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRR85302.1"
                     /translation="MRVLDRVYRESLTLLTDLYLLTMANGYWKLGRAEDEAVFHLFFR
                     KHPFGGGYTITCGLEYVLDLIRGFRFSNTDLEYLAGLRGNDDAPLFEPAFLDYLGDLR
                     LRCDIAAIPEGTVVFPQEPLIRVQGPLLECQLLETHLLNIINFQSLIATKASRICLAA
                     SGGTVLEFGLRRAQGMDGGLAASRAAYIGGCDATSNVLAGKLFGIPVRGTHAHSWVMS
                     FDGELEAFDAYAKAMPNNCIFLVDTYNTLEGVAHAIEVGRRLRESGHKMIGIRLDSGD
                     LAYLSIEARKMLDAAGFTDARIVASNDLDEETIRSLKDQEAAISIWGVGTRLITAHDE
                     PALGGVYKLSAIRRRGGAWKDCVKVSEQAMKTTAPGIQQVRRFRMGQEFIGDMIYDER
                     LGICGPPVMIDPVDPTRRKMMPADAEHEDLLVPVVIKGQVIYQSPAISDIRERAGRQL
                     AALHKGIKRFLNPHEYPTGLEQRLYDLKTKLVLQARNAPV"
     gene            10898..11995
                     /locus_tag="P5316_09855"
     CDS             10898..11995
                     /locus_tag="P5316_09855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR85303.1"
                     /translation="MKVPLLDLAAQFRTVEPEVRAGIDRVLKTCGFVLGPEVEALESE
                     VAQLCGVAHGIGVSNGSDAIVAALMALDVGAGDEVIVPVFTFFATAASVSRVGARPVF
                     VDILPDTYNIDPDAVAKAVTPRTKAIIPVHLYGQCADMDRVLTVADRHGIPVIEDAAQ
                     AIGATYKGRPACSFGKAATLSFYPTKNLSAIGEAGMVLTNDDKLAATLRVVRHQGQTS
                     EYEHGRLGANFRMSAIQAVALRAKLPHLAAWNDRRCRHAARYDAALAGNCVVTPHRDP
                     NCGHIYHQYTIRSPNRDALRARLTEAGIGAKIFYPIPLHLQPVYRNLGYRVGDFPVAE
                     KAAKEVLSLPVFPEMTAQQQDYVIETIRQAR"
     gene            complement(12016..14046)
                     /locus_tag="P5316_09860"
     CDS             complement(12016..14046)
                     /locus_tag="P5316_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85304.1"
                     /translation="MGKGDRGQYVIERAEAQQSATLIQSAALLGALVCVVLAGLLQDP
                     INRRREDLQLVMHSDIYKELPPEYGWISAFGGPLRGLAVHYFWYRAEELKQEGKYYES
                     QQLARWICTLQPRFAQVWIFQAWNMSYNISVATHTPQERWQWVYNGIRLLRDEGIPNN
                     DRVIALYRELTWIIFHKLGDRIDEFHNFYKRRWAATMENLLGPPPVGVSDEKILDWFR
                     PVAEAPVQLEEVMAKRPKVAELVKALGDLGVDVRSTTTNDKVFHPLEKLFFEPYARFL
                     HEKDMTGLRAQPAQTSEPQRRLNEFFAASAGEDFDALLAWLRAKVLREQYKMDPAFML
                     SMTEKLGTAKPIPLDWRTPWTHAIYWSMYGMKKGEEVHNVQEIDRLNTDRVMLYGLQN
                     LARMGRYIFRNNPDEPFRSFLETGPDLHFVEAMHNKYIELGKKYAEPDENVENRTCEM
                     LRDGHVNFLEEAVMNFYFAGRNDEARHYLDYLAKHYPDPNTNQPKEIYFKTVDEFMNN
                     RIGEIIDTQPSAIALIGNLLRRGYLALAGGSSDEFGAAVRRAAAIYQRYQEDAVSSEM
                     NRLRLPRFESIRAGALFRFVADATIPLYVRSFVWRYEGMTEENDLVRKYCYHFDMPGL
                     LKALGEECAEEGIDMKLAFPEPSGIEQWLKENPAPVLQEDLDKAPAGASKGG"
     gene            complement(14071..15477)
                     /locus_tag="P5316_09865"
     CDS             complement(14071..15477)
                     /locus_tag="P5316_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR85305.1"
                     /translation="MSRIWAVARHMIAESIRTKVALLFIAIILIILLTLPFTVTGDGL
                     TIKSRLQNYLAYSLGSVGALLSLLTVFLSCGTLSNEISGKYLFLVVSKPIPRWQLFVG
                     KWVGVVTLNGVMLLVTFAAALASTWYIKNRPATVPGDAEAVQKEVLEVRHNFRLHQPN
                     WNQLAQERLRQLREAGAIEKLSPEEEKALIARLIEEAKSGWRTLRPRQWHLFEFRLSE
                     IAVDRRRHADALEQDYVFLHIKPRHPGGTEDAVLQAVIACGDPREPQTLTREVTADFV
                     VERFHDIPIPTFAVNSRNTLYVQIFNLSDKDSITFEGDDCFELLYPLGTFHWNTLRAI
                     MITWSRLAFLAALGLMMSSFLSFPVACMASFLVLAAASMKKWLADAFEWTIPQGTRED
                     PMWIFGPPMRLIGRAFVNLVPDFTHYDAVSNIVGGRYVPAFWVLQALVLLLLVQGAVL
                     IIIGCVVLTRRELARVTA"
     gene            complement(15484..16677)
                     /locus_tag="P5316_09870"
     CDS             complement(15484..16677)
                     /locus_tag="P5316_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR85306.1"
                     /translation="MSSNDLSVIQTAGLTKVFKDFWHRQRVVAVDKLDLDIHKDEVFG
                     LLGPNGSGKTTTIKMLLGLLYPTRGRIQVLGRAPTDVEVKSRIGFLPEESYLYQFLDA
                     RETLDYYGRLFRIPRQERRRRVEQLLEMTGLGREARRRVGEYSKGMARRIGLAQALIN
                     DPDLLILDEPTSGLDPVGTREIKDLIVFLNREKRKTILLCSHLLADVEDVCQRVAVLY
                     GGRLQALGPIRELLSQENLTQITTEKLNPDSVDRIRRIIEREEGRQVISVSTPSDRLE
                     TFFLRIVRQAQQLRQTTSGATVGGRIAEFLTESPVGEAVVESLVAAASKPERVEPAAQ
                     PAAEPGESAATEVLKELISSSSAVAEATSPVENEPSAPVPQIDESVIDSLMNRADRKT
                     GPSKE"
     gene            complement(16718..18007)
                     /locus_tag="P5316_09875"
     CDS             complement(16718..18007)
                     /locus_tag="P5316_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRR85307.1"
                     /translation="MAQDHPHHEHIDPDHGHDYSSSEEPAGAAPAEAFDAANQALVDA
                     LKLSFRVLKAVMIGAVFLYVLSGLFIVDRDKEVVVQLRFGEQINTYGDGIHWAMPYPI
                     DELIEVPVSMDTLTLDAFWLQISDADKGKPLSELMARGDGLDPGVDGALLTGDRGIMH
                     LLTQAQYRITDAQQYVRNVVLQGNDRTGEKDLLRAVLKNACVASAARTTAEVISKDPS
                     TMVSGVKIRAQEMLDHLGAGITLDKVDVVQSWYPLQVQPSVDAVTNAMNTKQQAIQSA
                     YGERQKILNEAAGRVWEDLWNAIQELDQKATDQERSEVLGRIETLLTAQASGKAGKRI
                     QEARQQREQIVLSTLARQKAFLALLEEHRKNPELLRQRLLVEGLKDLFAQPGAVKWML
                     PGGENKQLDVWLNPDPVEFKKAQEAATRQRTGVGAGK"
     gene            complement(18048..19034)
                     /locus_tag="P5316_09880"
     CDS             complement(18048..19034)
                     /locus_tag="P5316_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRR85308.1"
                     /translation="MKRSVPSIVAAVAIVLILVFHMVTYQVRFNEVAVVRTFGKIQAP
                     DPVTGESPDVKTKPGLKFKWPWPIQQVSIFDNRIQLTETIGEETPTRDRKNVVVTTAV
                     GWSIADPYVFSIRNQDMKDAERKLQVRVRNDQKTVIARYDFAHFVSTNPDELKYDDIE
                     REIFRAIQETAPTASELFGIEVRYVGIEGLALPKQITTDVFNAMKKEREAEAARYTSE
                     GESEANRIKAEAESIAGTILAFADRQAEQIIAEGKARAAAYNETFRKDEDLAVFLLQM
                     DYLGKILKDRSTVVFDGHPPFDLLKEASLQGKPAAATRPAGRMPEPAIVEPK"
     gene            complement(19092..>20585)
                     /locus_tag="P5316_09885"
     CDS             complement(19092..>20585)
                     /locus_tag="P5316_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRR85309.1"
                     /translation="VFERVVAYILRIPLFVLAAETLLNLVLDFYRPRAPDEEPRPAFD
                     SRLLGLFTEPGGIARSIADAINYQFGFEVSSTWFYKLLQRSVVPLIGFAILMMFAASC
                     LVFVGADEEVVIERFGVKRDVLGGGLRIKWPWPIEKAYKVATAQVHELKIGILDEGTD
                     KDGKKELFLWTNKHAWEPHLNVLVATPKLAEYIEKTDGRVTTEPAEAQERRAQAVPVS
                     QLRVSATILYRIRDPYDWLRRYGGPDDKGPRAMLTAIANREIMRQCASVEVTALMGTS
                     RGAVERSLREEIQKTADQESLGVDIVFLGLQGVHPPSETAESFQAVIGAESKRTASIR
                     SAKADRDKRLAEVAGDVTRAENLNDAIRRLNELEASGKASGDELQEARDRVQRMFFGD
                     AKRGIRPVGGLAANRMAEARAVRWRIENQSAGQAIGFVHEMATRNAAPKVYRMRKYLE
                     MLAEATAGIRKYVLAASEAQGSLRTFHLNLQDARDIPIDVAIQKKSD"
BASE COUNT         3831 a         6704 c         5895 g         4155 t
ORIGIN      
        1 cgcctcgatg atatccgtcg accgcccacg gccgcgataa gtcttgccgt tgcacaccaa
       61 ctccagattg gcctcgccct gagcctcctt gccgccggtc acggctcgca agtcgaactc
      121 ctggagtttg gcctcgacgc cggtcaggcg cgtgatgcag gagaaagcgg catcgatcgg
      181 tccgtcgccg gtcgccgcgt ccaggtatgt cttgtcgtcg gccaggctcc tcaattgcac
      241 cgtggccgtc ggagtgacgc ccgtcccgcc catcgtctgg aggctgacca actcccaggt
      301 gcgcatggcc tcggcttcct cgaacaactg gccgacgatc gcttcgatgt cttcgtcgaa
      361 gacctccttc ttccgatcgg ccagcgtctt gaactgctca aagaccatat cgagctgctt
      421 gtcgtccagt tcgatcccca gttgcagcag ccggtcgcgg agggcgtgcc ggcccgagtg
      481 cttgcccaac accagcttgg actcgggaat ccccacgtcc tgcgggttca tgatttcgta
      541 ggtctcgcga tgtgccagca cgccgtgctg gtggatgccg gcctcatgag cgaacgcgtt
      601 ttgccccacg atcgccttgt tgcgctggac gtggatgccg gtgagcgagg cgaccagacg
      661 cgaggtcgga tagatcatct tcgtattgat ccgcgttcgc gccttgtaca cgtcggctcg
      721 ggtcttgatg gccataacca cctcttccag cgaggcgttg cccgcccgct cgcccaggcc
      781 gttgatggtg cactctacct ggcgggctcc ggctttcacc gccgccaacg aattggccac
      841 cgccagcccc aggtcgttat ggcagtgcac gctgatgacg gccttcttga tgctcttcac
      901 gtgggtcatc aggtactcga tgatgttcgt gaagtgatcg ggcgtggagt agccgaccgt
      961 gtcggggatg ttcacggttg tcgctccggc ctcgatcgtc gcttcaacca cttccgccag
     1021 gaacggcaac tcggtacgag aggcatcctc cggtgagaat tccacgtctt cgacaaagct
     1081 cttggccaac ttaaccccct cgacgctgag cttgatgatc tcggcctcgg cccgcttgag
     1141 cttgtattgc cggtggatgg ccgaggtggc gcagaacacg tgaatccgcg gtctggccgc
     1201 ttccttcacc gcctcggcgg cgcggacaat gtctttttcc gtacagcggg ccaacccggc
     1261 cacgctgcag cctctgacaa ttcccgagac ggcctgcacc gcctcgaagt catccggcga
     1321 ggtgatcgga aagcctgcct cgatgatgtc cacgttcagc cgttcgagtg cccgggcgat
     1381 ttccagtttc tgcgcggtgt tcagagacgc tccgggggac tgctcgccgt cccgcagcgt
     1441 cgtatcgaag atacgaatca tggattgacc gtcgcccgac tcttcctgtg atgtggacat
     1501 gacgttggct ccttgaggca gcttctccgc ggtacggccg cagatgcctc gaatacgaac
     1561 acgggttcac acgttagccg acagccggtc ggttgccaac tgccggcatg gcctttctgg
     1621 ccaaacaaac aaaaaagccc tacgcgacgc tgcgtagggc ttcgggtttc gctgcttttc
     1681 agaccggatc agccacccgc ggaccctacg cgcgcactaa gcaccaggct gagcaggagc
     1741 aagccttggc aggcgtagga accgggctgg gtgatacctg acctcatcgt agagaatcat
     1801 atcggcacaa gagggcgttg tcaacacaaa aaaggggtga gccttcaccg tcggtgatcg
     1861 gcccatcccc tgatgcctct cttggtcacg gtcgtgcgcc gcccaccccc tgcagacgca
     1921 ggggacgagc tttcagagtc tctcacaccc cgggttatcg cagatcaacc gcctcgtcgg
     1981 ggaccatgat catcggctcg acccagcccc cgcagtagtc gtagggtgga tcggccccgg
     2041 caaactcaac ctcggccgta atcgtggaca cctcgccgat cgcggcggct ttcaccccat
     2101 tcggggtgac cgaaaagacg ctatccctga tgaacacgcc ccgggtataa ccgccgtaat
     2161 agccccagta gcagccgttc acctgggaag tatcaacaga ggaaatgccg cctagatact
     2221 cgaagccgtt tgccacgctc acacgataga ccatcaaccc gctgaacttc caatcaccat
     2281 aggaccaggg gttgcccgac gtgccttcgt cgtacacgtc gatcggaaac gccaacaaac
     2341 cgtaatcctc gccttgaggc ttccacagat tgaacgcttt cgggttgtag ttcgcttcgc
     2401 tgttggttcc ccggccgccg atcctctcgc ggtactgtaa ctgtggatgc tccatgtcgc
     2461 tcacgtcgaa gaccgagagt tgcacgccct tgacccacgc ccaatcgccg tcaggctcgg
     2521 cgtccttgcc gatggtcagc aggtggttct catccagcaa ctgaatgtga tccgagtatc
     2581 ccggacagat cagttcaccc ttcatttgcg gcgacgtcgg ttcgctcagg tcgatgacga
     2641 acagcgggtc ggtccgccgg aacgtgacca cgaagccccg ctcgccgatg aaccgggcag
     2701 agtagatcgt ttcgccccgg ccgatgttct ccacgctgcc gacgatgttc aatgcgtccc
     2761 cctgttggcc gagtacgtac acgttgttgg tcgtgccgtt cccgttgccg ctgaagtcat
     2821 cctcggtcgt cgcgatgcga aggtagtcct ggtattcgcc gagcgagtac tgattcaggg
     2881 gacgccccgg taccagcccg ctggccgcgt actcggtgcc cgtctcggtg aagcggaact
     2941 tgtggatctc ggtatcggtt cgcgtgattt cccagaagcc ggaccagccg tattcggtgt
     3001 ccgttacgta aagcgcctcg gtgctcgcgt agatcgttcc tgcatcggcg acgatgctgg
     3061 tcgataccgg ctcgcccgtc gggtcgttga cgtccagcgt aaccacggtg gtgaggccgt
     3121 atccttgttc gtccagcgga cggaaaacgt tttcccagcc gacgatgtcg cccgactgct
     3181 tgccccccga ggcgttttca atctcgtaat ccggcaacca ctcttccagg gtgatttcgt
     3241 ctaactccct gaaggtaatg tcgtacggaa gccgcggcgt ggtggtcaga acaacgtgca
     3301 gcttgctctc gatcatgcgg ctggaggcca gcgaaccttc cagtcgaatc ttggcttcca
     3361 cggcggggtt ggccgggtcg gtcacgtcga tgatcgtcac cagcacctgg cttccgtcgt
     3421 tccatgggcc gccgacaata tcgtcctcgt cgagatcatc tgaggagtca tccccgtcat
     3481 cgcagcttgc gccgctatca tctccggatg tcgtgcagtc gccttctccc tccgaggcgt
     3541 cgctgccttc gcccggcggc tcttccgaac cttctccggt ctcagcaagg gactctctgg
     3601 cggcaccgaa gcgaaccgac gaatccatca aaagcggcat gggatagtac gtgtattcct
     3661 gcgataaggc gatcagcttg ttcccataga gatagagcga gtctccataa gagtcgagct
     3721 tgatgctggc tgcctcggcc aggggcgaaa cggaagcgat gtgcaccgtg ctctcgtcga
     3781 gtacgtagat gtaatccccg tcgttcttca ccagatcgct ctcatccacg ccggcctcct
     3841 ggatgttggt tccggaataa tcatcgcctc gatcagcccc gccgccctcg gctccgtttg
     3901 cggcgtcgcc accggtcgcc atatccgtgg gagccatcgg aaaaccgccc cagaagatcg
     3961 ggaatgggtt gcggtaattg tttctcatgg agttgctcgc ttgactggcg aagtagctcc
     4021 tgagctcttc gcccgacgag aaacccttga ggccggccac acgaggcttg ttattcagaa
     4081 agtccaggca attggcgcca ctgagcgaga acaagaaccc aatcgcaatc agcagtgaat
     4141 agcgtcgcat gaacgagacc ccctttctct attgcaggcg atggagcata cgttccacct
     4201 gcgcggcagg cttagtccaa ttataagacg acacaaggtg tccagaacct gacgtcgggc
     4261 acgggaaaac ccgcccggac ttcccttgat atccagatac ggcgtccacg cctctaatcg
     4321 accaaatgcg gcccacacaa gaacaaatgc aaacccttgt ccataataag gttgccaatc
     4381 acgccttggc caatacgcgg cggcgccggt ttcgggcggc tggccgccgt cgcacagatc
     4441 gatcctcggg tttgcccttt tccagatgcg agcacccgct gacacaggat gcgcgttgat
     4501 tcgccgcgct ttccgcacca tgatggtgct gccgtcttgg cggtgtccaa acgcaggaca
     4561 cgggctgatg tttcgtcagc cgggaagtct cgtgcttcct tacatccaga gcgacggatc
     4621 gcatccgtca gcaagacggt cgcgggcgca gccattggct cgcgcccgcg tccagttttg
     4681 aagatgacag ccatgttcgg ctttctgaaa tccctgtttt cacgacgtgt cgagccggcg
     4741 ttaccgcctc cgcgcttcac gaccgtcgat ctggcccggc gactcggcgt cgatgcggcc
     4801 gaactggccc gcgtcgatgt gagctacaag gcttttcagg ttcccaaacg cagcggaggc
     4861 atgagaacca tccaggcacc cgcccctccg ctcatggccg ttcaacgctt gatcctgcgc
     4921 aggttgttac gccgcctgcg cgcccacccc aacgccactg gtttcgaacg cggccactcc
     4981 atcgtcacca atgccctgcc gcacgtcgat aaggacatcg tcatccgtct cgacctggtg
     5041 aacttcttcc cctcgatctc ggccgatcgc gtcgagcgat acttccgctt catcggatat
     5101 gaccaggagg cggccaccat attgacccgt ctctgcacct ataagggctc ccttcctcag
     5161 ggggcaccga cgagtccgcg gttgagcaat ctggtcaact atcgcctcga tgctcgactg
     5221 gcggcacttg ccgagagccg aggcctctcc tattcacgat acgccgacga catcaccttc
     5281 tcggctcgaa tcgccgactt ggctgcgact cggtgcgcaa atcccaaagc gctggaaagg
     5341 caaaaacgcc ggcagcctcg cccgaacgat ttgatccatg ctgccaaggc cgtcattgcg
     5401 agcgagggct accgcctgca catgcatcgc aagctgcggg tttcccgtcg tcacgatcga
     5461 caactcgtca ccggcctggt ggtcaactcc aaacccgatt tgccgcgggc cacgcgtcgt
     5521 cgattgcggg cggtcgcgga tcaccttcgc aggggccggc cggcaacgct gacaccacag
     5581 caactggccg gttggcatgc actacaggcg atgatcgctg cccaaagggc aacgcctgcc
     5641 tcgtgacctt cgttcgggaa gacatgttgt ggccaatttc aaacgagatg tagaccggcc
     5701 gcttacacgg gccgttccgc tatcgcgaaa ggacgagctg atgagaagga caactgtgat
     5761 tggtctggcg tgtgcagtaa cgtcgagcgt tgtggccctg gcccaggacg caccggcgag
     5821 ccggccgggg gatgtgccgc aatgcgagat cgtctccgtc aagcggatct gggacgctgc
     5881 cccgcacaac gccttcaccg atctcatccg ctttcaggac aagtggtact gcaccttccg
     5941 cgagggcgag ggccatgtcg gcggcgacgg aaagctgcgc gtgctggagt cggtcgacgg
     6001 cgacaagtgg tcttccgcgg ctttgctgac tgaggagggc gtcgacctgc gcgacccgaa
     6061 gctttcgatt accgcggacg ggcgtttgat gatggttgcg ggcggctcga tctacaaggg
     6121 caaggaactg gttggccggc cgggaacaaa cctggtgaac gggcacctcg tgaacaatga
     6181 tcttctccga tccgtcgccc ctgccttgtg agaccttcct cagtccccga gccggcaaca
     6241 acgtaacctt ctcgtcggtc agcccggccg aagacgttcc tcttgcctct gctctcaccc
     6301 ttttacctgt cagtacggcg gcaggcaccg cctcaacaca tgggcgagca cgacgccgag
     6361 aaagaagacg tttcgcaaca ggacgtccgg ccagaaaagc ggcaactggc cctccatccc
     6421 cgcatgggtc ttcgcctcag acaggtgttc cccgagcgtt ctcatccact gcgttgttcg
     6481 aggccactcc gcaagcccat ccagccattc cgcgggaatc gcctcaacac ccagcgtcgc
     6541 acccatgagc gcccccacaa tggcacccgt cgtatccgta tccccgccaa gcaacaccgc
     6601 cgcctccacg gcagatcgga aatcgtcggg ccgccgcagc cagcaataca atgccaccgg
     6661 cacggtgtgg ttgatgtagc cgctcacgcc gtgacgaagg ccgagcgcca cggcgtattc
     6721 tgcggcatca tctccttgct gaagatgatc gttggctcgg cttagcaagt gcatcaacgg
     6781 cgcatccttc agatacttct ccgcgtgcgc caggaactct gccggccgaa caccctcgct
     6841 gcctctcatg gctccgtaac gtgccgccag ggccacggcc agcgcaccat gctcggccgc
     6901 cgggtcgcag tgggtgatca ccgtcgacgc gtgtaccagc ctcagcagca gatcgtcatc
     6961 atcaccggcg tagactccca ggatcgccga tctcatggca ggtccgttgc ccgcggacca
     7021 gactccgctg cgatgaggcg ggaatcccaa ccacagcttg agaatcgatc gcgcggtcgc
     7081 cgaacccacc ccggccggta agccaaggag ccaccatcgc agcttccagg ccagcgatcg
     7141 agcaaaagcg tccgggtttc ccttcgatgc cagcagcgcc tgcgcaacca tacaggtgtg
     7201 ctcagtgtcg tcgctgacca ttcctcgccc tatcagcaag cggtgatcga gaggggcaga
     7261 gccgaagagt ctcgccgccc gccgggcgga caggccttcc cgcggcagac cgatggcatc
     7321 accgacggcc gtcccgagga tgcaaccttg gaaggcgcca gagaagagcc ttgaatcgtt
     7381 gatgctgacc atcagagttc tccgagagct tcaggtctcc ggccaagtca ggtactccgg
     7441 cggcacatga tcagtgagcc acacgttgtt gccggttcgg taaaacacat ggccatcctg
     7501 gtgcattttc cgtgcgtcga tcttcagaag gacgggcttg ccgtatctgg acgcgacctt
     7561 cagggtcatc actgtctcgg ccgacaggtg gacgtggtgc cgtttgccct tgagcagtcc
     7621 cttgtcacga atcgattcga ggttgtgcac ggccgtgccg tgatacaaga cctcgggtgg
     7681 ctcgaccggg acgtatccga gatcgacctt aatcgagtgc ccctgcctcg cgcggatcat
     7741 cgcttggtcg tcgctgaact caaagcgctg cttgtcattg gtcaggacga cctcctccag
     7801 ttgctcccag gaaataggcc ggccgtgcgc ctcgcactga accagcaaca catccaccga
     7861 cacccagccc gcgcgatcca gctccaggcc gatctcgtca ggccggtgcc gcaacacgta
     7921 gctgagaaac ttgctcgttc tcttcttttc ttcgctattc atatctcgaa attgaacccc
     7981 ttcttcgtca gccggtcata gtgcttcttg tcaaagcgat acaaccgggc ggcccggtgg
     8041 gccacgtcgg tctcgacttc gtcgagttcg ataagaatcc ccatgcccag gatcttcttg
     8101 cgaaagttgc gtttatcgag cggccgatcg agcacctttt cgtacagatg ctgcagttgc
     8161 cgtagcgtga acttcggcgg cagcaactca aagccgatcg gctggtagcg caccttgccg
     8221 cgcaaccgct gcaaggccat tcgcaggatg cgagcgtgat cgaacgccag cttagcgcag
     8281 tcttcgacgg cgaaccaggc ggcgttgcgg gcgtcggtgg cggcctgtac gcgatggtcc
     8341 cgcagattga ccagcgcgta gtaggccacg gtcaccactc gctcgcgcgg gtcgcggccc
     8401 aggtcgccaa acgtgtacag ttgctcgagg tacacgtccg agagcccggt ttcctcgcgc
     8461 agctcccgct cggcggcctc ctccaacgtc tcatccattc gcacgaaacc gccgggtaag
     8521 gcccactgcc cctcgaaggg cggcaggtca cgctggatga gcaacacctt gaggtcctcg
     8581 tcatccaggc cgaagaccac gcaatcaacg gtcaggctcg gacgagggta ctggtacgtg
     8641 tgcggcatgg gtcgttcctt gcttttcagt gtaatctaca cgcttagcat agtgtatatt
     8701 tcacactaag tcaaataaga atccggtttc gttgatttct ccctcaaagg ctcgcagggt
     8761 cagaccggcg cgttcctcgc ttgcagcacg agcttcgtct taaggtcgta aagcctctgc
     8821 tccaggcccg tcgggtactc gtgcgggtta aggaaacgct tgatgccctt gtggagtgcg
     8881 gccagttgcc gcccggcccg ctcgcgaatg tcgctgatgg caggcgactg atagatgact
     8941 tgccccttga ttaccaccgg aaccagcaga tcttcatgtt ccgcatcggc cggcatcatt
     9001 ttgcgccgcg tcggatcgac ggggtctatc atcaccggcg gaccgcaaat gcccagcctt
     9061 tcgtcatata tcatgtcccc tatgaattcc tgccccatcc gaaaccgcct gacctgctgg
     9121 atgcccggcg ccgtggtctt catagcctgt tcggagacct tgacacagtc cttccacgct
     9181 ccgccccgcc gtcggatcgc tgagagcttg tagacgccgc ccaaggccgg ctcgtcatgg
     9241 gcggtgatca ggcgcgtccc gacgccccag atcgagatcg cggcctcttg gtccttaagg
     9301 ctgcggatcg tttcctcatc gaggtcgttg cttgccacga tgcgggcgtc cgtgaagcct
     9361 gccgcatcaa gcatcttgcg agcctcgatg ctcaggtatg ccagatcccc cgagtcgagg
     9421 cgaatgccga tcatcttgtg gccgctttcc cgaagccgcc gtccgacctc gatcgcatgg
     9481 gccacgcctt caagcgtgtt gtaggtatca accaggaata tgcagttgtt gggcatcgcc
     9541 ttggcgtagg catcgaacgc ctccagctcc ccgtcgaacg acatcaccca actgtgggca
     9601 tgggtccctc gaaccgggat gccgaatagc ttgcccgcca gcacgttcga agtggcatcg
     9661 caaccgccga tgtaagcggc ccggctggca gccagtcccc catccatgcc ttgcgctcgg
     9721 cgcagaccga attccagcac cgttcccccg ctggcggcaa ggcagatccg cgacgccttg
     9781 gtggcgatca gcgactggaa gttgatgatg ttgagcaggt gcgtctccag caactggcat
     9841 tcaagcagcg gaccctgaac ccgaatcagc ggctcttgcg gaaagacaac ggtgccttcg
     9901 ggaatggcgg cgatgtcgca gcgaagcctc agatcgccga gataatccag gaatgccggc
     9961 tcaaacagcg gtgcatcgtc gtttcccctg agcccggcca ggtattcgag atccgtgttg
    10021 ctgaaacgaa acccccggat caggtcgagc acgtattcca gcccgcaagt gatcgtatag
    10081 ccgcccccga aagggtgctt tcggaagaac aggtggaaga cggcctcgtc ctcggcccga
    10141 cccagcttcc agtagccgtt tgccatggtc aacaggtaca ggtctgtcag caacgtcaaa
    10201 ctctcccggt agacccgatc gagaactctc atgttggcct ccttgcgggt gtgttccgca
    10261 aaccaagtat ccgctcctgt aaagccgacc ataatccatg accttggttc gggacagctt
    10321 ttccacgcca aactgacgca tttccgtcca gagaagcgat gaatctcgag cttcttgcct
    10381 aaacattgat ggatcaagac cttcggtgaa cgtcctataa ctgagacggc acggcgcctc
    10441 cgcagcccgg ccataccagc aaaaaatggt gaaaaaaacg cacgattctc aggcccggtc
    10501 caagtatcgc agacccaacg acttgtgaag gacgggaatc actcacaacg agacgctatg
    10561 cacaacccat tatctgtaat gaagttacgg ttttcatggt ttctgatcgt ttcgccgatg
    10621 cattcgttga catggccgcc gggacagcgg tatagtcggt cgcacacgca tggatgaata
    10681 tcgtgtcggc gactctatcg cgcgtcgtcg gtacgtcaac ggaacagggt ttcaccccag
    10741 atattcggct gtcttagccg tggcgcactt ccgggaccga ataccaattc gccccggaag
    10801 ttttttttgc gcagtgcgcg cttgctgcgc ctccggcaag caatgtccca gaacgttcag
    10861 gttgtcgcga ccaggcaaaa cgcggatact gtgccgcatg aaagtaccgt tacttgattt
    10921 ggctgcacag tttcgcaccg tcgagccgga ggttcgcgcg ggaatcgacc gggtgctgaa
    10981 gacctgcggc ttcgtcctcg gacccgaggt cgaggctctt gagagcgaag tggctcagct
    11041 ctgcggcgtg gcccacggca tcggtgtcag caacggttcg gacgccatcg tcgctgctct
    11101 catggctctc gatgtcggcg cgggtgatga ggtcatcgtg ccggtgttta ccttctttgc
    11161 aaccgcggcg agcgtttcgc gcgtcggagc aagaccggtc ttcgtggaca ttcttccgga
    11221 tacgtacaac atcgatccgg atgccgtcgc caaggccgtt acaccacgaa ccaaggcgat
    11281 tattcctgtt cacctctacg gccaatgcgc cgacatggac cgcgtcctca cagtcgccga
    11341 ccgtcacgga atccccgtaa tcgaggatgc cgcgcaggcc attggcgcga cctataaggg
    11401 caggcccgcg tgcagttttg gaaaagcggc caccctttcc ttctatccga cgaaaaacct
    11461 gtccgcgatc ggcgaagccg ggatggtgct gaccaatgac gacaagctgg ccgcaacgct
    11521 gcgagtcgtg cgccaccagg gacagacttc cgagtatgaa cacgggcggc tgggggccaa
    11581 tttccgtatg agcgccattc aggcggttgc cctgcgggcg aagttgccgc acttggccgc
    11641 ctggaatgac cgtcggtgcc ggcacgccgc ccgatacgat gccgccctgg ccggcaattg
    11701 cgtcgtcacg ccgcacaggg accccaactg cgggcacatc tatcaccaat ataccatccg
    11761 ctccccgaat cgtgacgcgt tacgagccag gctgacggag gccggcatcg gggccaaaat
    11821 cttctatccg atcccgctcc atctccagcc ggtctaccgc aatctgggct accgcgtggg
    11881 tgacttcccc gtcgccgaaa aggccgcaaa ggaagttctc tcactgccgg tgttcccaga
    11941 aatgaccgcc cagcagcagg actacgttat cgagacaatt cggcaggcca ggtgaacgct
    12001 tccgtgcaag cggactcatc cccctttgct ggcgcctgcc ggggccttgt ccaggtcctc
    12061 ctgaaggacc ggagcgggat tctccttcag ccactgctcg atgccggagg gctcagggaa
    12121 cgccagcttc atgtcaattc cttcttccgc gcattcctcg cccagggctt tgagcaggcc
    12181 gggcatgtca aagtggtagc agtacttgcg gaccaggtca ttctcttccg tcattccctc
    12241 gtatcgccac acgaacgagc gaacgtaaag cgggatcgtt gcgtcggcca cgaagcggaa
    12301 caaggccccg gcccgtatcg actcgaatct cggcagacgc agacgattca tctcgctgct
    12361 gacggcatct tcctggtatc gctggtagat ggcggccgcg cgacgtacgg ccgctccgaa
    12421 ttcgtcgctg ctgccgccgg ccagcgcgag gtaacctctc cgaagcaggt tgccgatgag
    12481 tgcgatcgcc gaaggctgcg tgtcgatgat ctcgccgatc cggttgttca tgaactcgtc
    12541 caccgtcttg aaatagatct ccttgggctg gttggtgttc gggtcggggt agtgcttggc
    12601 caggtaatcc agataatgac gagcttcgtc gttccggccg gcgaagtaga aattcatcac
    12661 cgcttcctcg aggaaattca cgtgcccgtc tcgcaacatc tcgcaggtgc ggttctcgac
    12721 gttttcgtcc ggctcggcgt atttcttccc cagctcaatg tacttgttgt gcatcgcctc
    12781 cacgaagtgc aggtcgggac ccgtttccag gaacgacctg aacggctcgt ccggattgtt
    12841 ccggaagatg tatcgcccca tccgcgccag gttctgcagg ccgtagagca tcacccgatc
    12901 cgtgttcaga cggtcgattt cctggacgtt atgaacctct tcgcccttct tcatgccgta
    12961 catcgaccaa tagatggcgt gcgtccacgg ggtgcgccag tcgagcggaa taggcttggc
    13021 cgttcccagc ttctcggtca tcgacagcat gaatgccgga tccatcttgt actgttcgcg
    13081 cagtaccttc gcccgcagcc acgccagcag ggcgtcgaag tcctcaccgg ccgaagcggc
    13141 aaaaaactcg ttcagccggc gttgcggctc gctcgtctgg gccggctggg cccgaagccc
    13201 ggtcatgtcc ttctcatgaa gaaaccgggc gtacggctcg aagaaaagct tctccaacgg
    13261 atgaaacacc ttgtcgtttg tcgtcgtact ccgcacgtcg acgccaaggt cgcccaacgc
    13321 tttcacaagc tcggccacct tcggtcgttt ggccatgact tcttccagtt gtaccggcgc
    13381 ctcggccacc ggccggaacc agtcgagtat cttctcgtca ctcaccccca ccggcggcgg
    13441 accgagcagg ttttccatcg tcgccgccca tcttcgcttg tagaaattgt ggaactcgtc
    13501 gatccggtct ccgagcttgt ggaagatgat ccaggtgagc tcccggtaca acgcaatgac
    13561 ccggtcattg ttcgggatgc cctcgtcgcg cagcagtcga ataccgttgt acacccactg
    13621 ccagcgttcc tggggcgtgt gcgtcgctac ggagatgttg tacgacatgt tccaggcctg
    13681 gaagatccat acctgcgcga aacgcggctg caacgtgcag atccaccggg ccaactgctg
    13741 tgactcgtag tacttgccct cctgcttcag ctcttcggca cgataccaga aatagtggac
    13801 cgcgaggccg cgaagcggtc cgccgaaagc gctgatccat ccgtattccg gcggcagctc
    13861 cttgtagatg tccgagtgca tgaccagttg gaggtcctct cgccgccggt tgatcggatc
    13921 ctgcagcaag ccggccagga cgacgcagac aagcgcgccg agcagcgccg ccgactggat
    13981 caaagtggcg gattgctgtg cctcggcacg ctcgatcaca tattgaccac gatcgccctt
    14041 gcccactgac aaatctctcc ttcagaccgc ctaggcggtc acgcgagcca gctcccgcct
    14101 ggtcaacacc acgcacccga tgatgatcag caccgctccc tggaccagca gcaacaggac
    14161 aagagcctgc agcacccaga atgctggcac ataccgccct cccacgatgt tgctcaccgc
    14221 gtcgtaatgc gtgaaatcgg gcaccaggtt gacgaaggcc cggccgatca gacgcatcgg
    14281 cggaccaaag atccacatcg ggtcttcccg cgttccctga gggatcgtcc attcaaaagc
    14341 gtcggccagc cactttttca tcgaggccgc cgcgagcacc agaaacgagg ccatgcaggc
    14401 cacggggaac gacagaaagc tcgacatcat cagtcccaac gccgccagaa acgccagccg
    14461 cgaccaggtg atcatgatcg cccgcaaggt gttccagtga aacgtcccga gcggatagag
    14521 caattcgaaa caatcgtcac cctcgaaggt gatcgagtcc ttatcgctca gattgaagat
    14581 ctggacgtac aacgtgttcc ggctgttgac cgcgaacgtg ggaatcggga tgtcgtgaaa
    14641 ccgttccacc acgaagtcgg cggtcacctc gcgggtcagg gtctgcggtt cccgtggatc
    14701 accgcacgcg atcacggctt gcagaacggc gtcttccgtg ccgcccggat ggcggggctt
    14761 gatgtgcagg aacacgtagt cctgttccag ggcgtcggcg tgcctccgcc tgtcaaccgc
    14821 gatctcggaa agacggaact cgaacaggtg ccattgtctt ggccgcagcg ttctccatcc
    14881 gctcttggct tcctcgatga gccgcgcgat aagggccttc tcttcttctg gagagagctt
    14941 ctcgatggcc cctgcttccc gcaattgacg aagccgttcc tgggcgagct gattccaatt
    15001 cggctgatga agcctgaagt tgtgccggac ctccagcacc tccttctgca cggcttccgc
    15061 gtcgccggga acggtcgcag gtcgattctt gatgtaccac gtcgaggcca acgccgcagc
    15121 aaaggtcacc agcagcatca ccccgttcag ggtcaccacg cccacccatt tgcccacgaa
    15181 caactgccat cgcgggatcg gcttgctcac caccaaaaac agatacttcc cgctgatctc
    15241 gttcgacagc gttccacagg ataaaaagac ggtcagcaga ctgagcagcg cgccgaccga
    15301 gcccagcgaa taagcaaggt agttctgaag ccggctcttg atcgtcagcc cgtcgcccgt
    15361 caccgtgaac ggcagggtca gcaggatgat cagaatgatc gcgataaaca acagcgcgac
    15421 cttggtccgg atgctttcgg cgatcatgtg gcgggcgacg gcccagatgc gcgacatgcc
    15481 gggtcactcc ttggacggac cggtcttgcg gtcggcgcga ttcatcaggc tgtcaatcac
    15541 gctttcgtcg atctggggaa cgggggcgga cggctcgttc tcgaccggcg aggttgcctc
    15601 ggcaacggcg gacgacgagg agatcagctc tttcaacacc tcggttgctg ccgattcacc
    15661 gggttcggcc gcgggctggg cggcgggctc gacccgctcc ggtttggagg cggcggcgac
    15721 cagcgactcg accaccgctt cgccgaccgg cgattccgtc agaaactcgg cgatgcggcc
    15781 gcccaccgtg gcaccggatg tcgtctggcg aagctgctgg gcctgccgga caatccgcag
    15841 gaagaacgtc tccagcctgt cggacggcgt cgacacgctg atgacctgcc ggccttcctc
    15901 acgctcgatg atacggcgaa tgcggtcgac gctgtccggg ttgagcttct ccgtggtgat
    15961 ctgcgtcagg ttctcttggc tcaacagctc ccggatcggc ccaagagcct gcaaccgccc
    16021 cccgtaaagc accgccactc gctggcacac gtcctcgacg tccgccagaa ggtgactgca
    16081 cagcagaatg gtcttgcgtt tctcgcgatt caggaagacg atcagatcct tgatctcgcg
    16141 agtgcccacc ggatccagcc ccgacgtcgg ttcatccaga atcaataaat cggggtcgtt
    16201 gatcagggct tgggccaggc cgatacgccg ggccatgccc ttcgaatact cgcctacccg
    16261 ccgccgggcc tcgcgaccca gacccgtcat ttcaagaagc tgctccacac gccggcgccg
    16321 ctcctgacgc ggaatccgga acagacgccc gtagtagtcc agcgtctcgc gggcatcgag
    16381 aaactggtat aggtacgact cctccggcag aaaaccgatc cgcgacttga cctccacgtc
    16441 ggtcggcgca cggccgagaa cctgaatccg ccctcgcgtg ggatacagca gccccaacag
    16501 catcttgatc gtggtcgtct tgcccgatcc gttggggccc agcaagccga agacctcgtc
    16561 cttgtggatg tcgagatcca gcttgtccac cgccaccacg cgctgccggt gccagaagtc
    16621 cttgaagacc ttggtcaacc cggcggtttg aatcacggat agatcgttgc tggacataga
    16681 tggtccgctc aatggttgtg gtttctgcct cgcgggctca tttgcctgcg ccgactcccg
    16741 ttctctggcg agttgcggcc tcctgtgctt tcttgaattc caccgggtcc gggttgagcc
    16801 acacatccag ttgcttgttc tcgccgcccg gcagcatcca tttgaccgcg ccaggctggg
    16861 caaacaggtc cttgagtcct tccaccaaca accgctggcg aagcaattcg gggttcttcc
    16921 ggtgttcctc cagcaaggcc aggaaggcct tctgccgggc cagcgtcgac aacacgatct
    16981 gctctcgctg ttgccgggct tcttggatgc gcttgccggc cttgcccgaa gcctgagccg
    17041 tcagcagcgt ctcgattcgg ccgaggacct cgctccgctc ctggtcggtc gctttctggt
    17101 caagctcctg gatcgcgttc cacaaatcct cccagactct ccccgccgcc tcgttcagga
    17161 tcttctgccg ctcgccatag gccgactgga tcgcctgctg tttggtgttc atcgcattgg
    17221 tgacggcgtc gaccgacggc tgaacctgca aaggatacca ggactgaacg acgtccacct
    17281 tgtcgagcgt gatgccggca cccagatgat caagcatctc ctgcgcccgg atcttgacgc
    17341 ccgagaccat ggtgctggga tctttcgaga tgacctcggc cgtggtccga gcggcgctgg
    17401 ctacgcaggc gttcttgagc accgctctca gaaggtcctt ctcgccggtc cgatcgttac
    17461 cctgaagaac aacgttcctg acgtattgct gggcgtcggt gatgcgatac tgggcctggg
    17521 tcaacagatg catgataccc cgatcacccg tgagcagggc cccgtccacc ccagggtcta
    17581 gcccgtcgcc gcgcgccatc aactcgctca acggtttccc cttgtcggca tcgctgatct
    17641 gcagccagaa cgcgtccagc gtcaacgtgt ccatcgaaac gggaacctcg atgagctcgt
    17701 cgatcggata aggcatcgcc cagtggatgc cgtctccgta agtgttgatc tgttcgccga
    17761 accggagctg aacgaccact tctttgtccc gatcgacgat gaacagaccg ctcaacacgt
    17821 agaggaaaac cgctccaatc atcaccgctt tcagaactcg aaagctgagc ttcagcgcgt
    17881 cgaccagcgc ctggttcgcg gcatcgaacg cttcggcggg ggccgcaccg gccggctcct
    17941 ccgaagatga gtagtcatgc ccgtgatcgg gatcgatgtg ttcgtgatga ggatggtcct
    18001 gagccactgt tgtcactccg tgccgggccg ccaggccctg cgagtcctca cttcggttca
    18061 acgatggccg gctcgggcat acgccccgca ggccgcgttg cggccgccgg cttgccctgc
    18121 agactggctt ccttcagcag gtcaaagggc ggatgaccgt cgaaaaccac cgtcgagcgg
    18181 tccttcagta tctttcccag atagtccatt tgcagcagga acaccgccag atcctcgtcc
    18241 tttctgaagg tctcgttgta ggccgccgcc cgtgctttgc cctcggcgat gatctgctcg
    18301 gcctgccgat cggcgaaagc caggatcgtg ccggcgatgc tctccgcctc ggccttgatg
    18361 cgattggcct ccgattcgcc ctcgctggtg tagcgggccg cctccgcctc gcgctccttc
    18421 ttcatcgcgt tgaagacgtc cgtggtaatc tgcttgggaa gcgctagccc ctcgatcccg
    18481 acgtatcgaa cctcgatgcc gaaaagctcg ctcgccgtcg gcgccgtctc ctgaatcgcc
    18541 ctgaaaatct cccgctcgat gtcgtcatac ttcagttcgt ccggattggt tgagacgaaa
    18601 tgggcgaaat cgtatctcgc gatgaccgtt ttctgatcgt tgcggacccg aacctgcagt
    18661 ttccgttcgg catccttcat gtcctgattg cggatgctga aaacgtaggg atcggcgatc
    18721 gaccagccga cggccgttgt cacgaccacg ttcttgcggt cccgggttgg ggtctcctcg
    18781 ccgatcgtct ccgtcaactg gattcggttg tcgaaaatgg aaacctgctg gatcggccag
    18841 ggccatttga atttcaggcc gggcttggtc ttcacgtcgg gcgactcgcc cgtaaccggg
    18901 tcaggcgcct ggatcttgcc gaaggtcctc accacggcca cctcgttgaa acgcacctgg
    18961 taggtaacca tgtgaaaaac gaggatgaga acgatcgcaa ccgccgcaac gatggatggc
    19021 acactgcgct tcatgagtca tgtctctccg atactcgtga ttcgggttct ctgtccccct
    19081 gtccgatccg atcaatccga cttcttctgg atggccacgt cgatcgggat gtcccgtgcg
    19141 tcctggaggt tcaaatggaa ggttcgcagc gaaccctggg cctcagatgc ggccagaacg
    19201 tatttccgga tgccggcggt ggcctcggcc agcatctcga ggtacttacg catgcggtat
    19261 accttcgggg ccgcatttcg ggtcgccatc tcgtgaacaa agccgatcgc ctgcccggcc
    19321 gactggttct cgatccgcca ccgaacggcc cgggcctcgg ccattctgtt ggccgcgagc
    19381 ccgcccacgg ggcggatgcc ccgtttcgcg tcgccgaaga acatacgctg aactcggtcg
    19441 cgggcttcct gaagctcgtc cccagacgcc ttgccgctgg cttccagctc attgagccgg
    19501 cggatggcgt cgttgaggtt ttcggcgcgg gtgacgtcgc ctgcgacttc agccagtcgc
    19561 ttgtcgcgat ccgctttggc actgcgaatc gaggccgtcc gcttcgactc ggccccgata
    19621 accgcctgga aggactcggc cgtctccgac ggcgggtgca ctccctgcag accgaggaaa
    19681 acgatgtcga cacccaggct ctcctgatcc gccgtcttct gaatctcctc ccgcaggctt
    19741 cgttcaaccg ccccacgcga cgtgcccatc aaggcggtga cctccacgct ggcgcattgc
    19801 cgcataatct cccggttcgc gatggccgtc agcatcgccc gaggcccctt gtcgtccggt
    19861 ccgccgtatc gccgcagcca gtcatagggg tctcggatcc tgtagaggat ggtggccgag
    19921 acccgcagtt gactcaccgg gacggcctgg gcccgccgct cctgggcctc ggccggctcc
    19981 gtcgtcacgc ggccgtcggt cttctcgatg tactccgcca gtttgggcgt ggccacgagc
    20041 acgttgaggt gcggctccca ggcgtgcttg ttcgtccaga ggaatagctc tttctttccg
    20101 tccttgtccg tgccttcgtc gaggatgccg attttcaact catgcacctg cgcggtggcg
    20161 accttgtagg ctttctcgat cggccaaggc cacttgatcc gcaggccgcc ccccagcacg
    20221 tcccgtttga ccccaaaacg ctcgatcacc acctcctcgt cggcacccac gaataccagg
    20281 caggacgccg caaacatcat caggatcgcg aatccgatca acggcaccac tgaacgctgc
    20341 aacagcttgt aaaaccaggt cgacgaaacc tcgaaaccga actggtagtt gatcgcatcg
    20401 gcaatcgagc gggcgatgcc gccgggttcg gtgaacaggc ccagcagccg gctgtcgaag
    20461 gccggccgcg gctcttcatc cggtgccctg ggccgataga agtcaagcac caggttcagg
    20521 agcgtctccg ccgccagcac gaacagcgga atgcgcagaa tgtaggccac cacccgctcg
    20581 aaaac
//
LOCUS       DAOTPW010000090        20192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2719_length_20192_cov_5.280727, whole genome shotgun sequence.
ACCESSION   DAOTPW010000090 DAOTPW010000000
VERSION     DAOTPW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20192
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2719_length_20192_cov_5.280727"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="P5316_09890"
     CDS             complement(<1..1390)
                     /locus_tag="P5316_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85310.1"
                     /translation="MAVLLSGSLSVVLAQDAADSPPAFAATLGARYVDGSFGFAITPP
                     TGCETLREKRFMETINEVEIVRFVDVNGRWSLGVRQSKPERALDPEALAEMVIGEMQE
                     TYPDITVAKTDRSTVGSREILRCKALMTARGVPMIRLQAMIRVKPSEYFTLVFAAPQS
                     DEKAAIDVFDKIVTGFEVLRSEAQEEQIRDALRRGSALLDLVRSGALDVVGREPREVF
                     LRCLEGGVEIGFMQIRMEPRTVEHRKGVTVAKWAWFFRPDQSIALMQQDMFVTSNLSF
                     ERWESRLIAISPPPKGGGRREISQDIESGIRQETKLLVGYAGKSFGPQMTQKELDVDQ
                     TYASAPWDLLFPSIVDLKKTELYAFSWFDSSRRGLSLQTFRLVGRKTMPGGKEATLIE
                     QSEGLIPPVHEIYVDDDGQIVRVVMKVGDKPLEMHALARRDIERRYGDRVKEVERLMP
                     GPVTAPADQKAAN"
     gene            complement(1480..3270)
                     /locus_tag="P5316_09895"
     CDS             complement(1480..3270)
                     /locus_tag="P5316_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85311.1"
                     /translation="MAAIALLLLAAGAAQLLIWHREKTKLIELGSSVHYPEGQLSVSL
                     PLGWQTLDKALCPAGTVIAVEDRETEGRQRRLYVFRGPPSSEGLAASASLRALDSFSN
                     ALGADVLFEKEPETAQVGAFPGVTATMAKRLGAVGVKPPFCLGRAAVGPGGEVVGVLL
                     ETALPSRRTDQLLVQRVAETVELTSIGLDPDPAEAMAAAGISFDIPRGARFIGPIERG
                     LPRVRLMSRQTAPAWYLDLFRVPLIARRKVNDLVEDGAMSLLQQVRLGEPLEPFDPGG
                     RQAARASLVITPGKEPTVHLVAVKTDQHTALLMVGRHDAEAGTAVRELCDSIAATARV
                     ESYDELVDLESAIDAARRLIKDCRKEGMSSLLGRREGRRLRFNVEAPALGLGSWIETC
                     RRSSDNAELPWEVTGRWVYDPRWRGHARIVVTEGWRLAENGVEYLYRFERSDAGEKSI
                     EQTEHSDAEGRQVTRIVKAFGGSRTEEIEVDDAFASGPLLTELCARTAGSSSQRSVVV
                     STIDTFTAGVAYCVIKPLGRTPVPGTPMREPARTVRLMMDYDPEPMLMYFDDGDELLA
                     VSHDARQWRKRVQPPSEAEEQPGVGPGLRR"
     gene            3646..3948
                     /gene="raiA"
                     /locus_tag="P5316_09900"
     CDS             3646..3948
                     /gene="raiA"
                     /locus_tag="P5316_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRR85312.1"
                     /translation="MQISVTGRHIEFTESMRQYAEEKLSKLARFYDRIDRVEVVLEQE
                     PTKHRLEVVMHADHKTTFVAQVEAGEYHEAVDLIVEKLERQLKKHKEIYRNRKHQS"
     gene            4021..4494
                     /locus_tag="P5316_09905"
     CDS             4021..4494
                     /locus_tag="P5316_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRR85313.1"
                     /translation="MKLSDFFVPEAVIPELKATDRNGVIRELTASLAAAVGMDEAQAA
                     AISKAVIARENQGSTGFGKGVAVPHVKHPAVKRIMATLGRSSTGVDFAALDRAPVYIV
                     VLLLSPPDNPDQHLASMENIFWHLQRDNFRRFLRQASTKEEINENIREADELPGG"
     gene            4559..4846
                     /locus_tag="P5316_09910"
     CDS             4559..4846
                     /locus_tag="P5316_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRR85314.1"
                     /translation="MNETAVPEASRRVTIENSLGLHMRPTMQFVDLANQFRSRVVVRK
                     GKHAVDGKNPMEMMLLEAVRGTELEIQAAGADAEDAVEALAALVKSGFGEQ"
     gene            4913..6652
                     /gene="ptsP"
                     /locus_tag="P5316_09915"
     CDS             4913..6652
                     /gene="ptsP"
                     /locus_tag="P5316_09915"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRR85315.1"
                     /translation="MEIKKGIGVSPGVTIAKAVVLDAEEYRIAQKTVSVDDIPAELER
                     LRSGFDASLAELHSLQEVMARQLGPDIAAIFDFHYGVLSQDRLFEQIAALITQKNYSA
                     AYAVREVLRGYQRRFLNMKDQLLRERVRDVNDIERRLLRHLCGQSGEELSLLTEPFVL
                     VAHDLTPSQVANLSQTKVSGVAMDAGGLTSHAAIVVRSMGIPAVMGLKDVSTRVSKSD
                     TIILDGTKGLVVVRPDADMLQEYRVEEERIHALASDLIELRDKPAVTTDNIKITLLTN
                     IEFPYEAKTCLEKGAEGIGLYRTEFLYLRSEAEPTEEEHYEAYHTVIQAVGDYPVTIR
                     TLDLGADKYTQSQAREPERNPFLGLRSIRYCLQNLDLFKTQLRAILRASVEGDVRIMF
                     PLISSLMELRQAKIVLGDAMEDLEEAGIPFRRDIPVGIMVETPAAALQIKELLREVDF
                     ISIGTNDLIQYTLAVDRSNERVASLYTASHPVVLRMLRDIMRAAERAGIPYSMCGEMA
                     GEPMYTLLLLGIGLRQFSMAPSDIPEIKKIIRSASIAHAVRVAKRALSFDTDRQVSNY
                     LRDETRKIWPEFM"
     gene            6831..7463
                     /locus_tag="P5316_09920"
     CDS             6831..7463
                     /locus_tag="P5316_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRR85316.1"
                     /translation="MQYRVAIRFVIDGDIRFISHHDTMRLFERALSRAQLPVRFSRGF
                     NPRPRLSLPLPRAVGIAGAAELLVVDLEEPLDPAVVLDKLREQMPAGLTLSEARSIAG
                     KHPPQAVRADYALALPPDRAKEVGQRMKDLLAASMWNIQRSGPGTKRPRELDVRQYLA
                     GGAVCEGILRWTVRVTGAGSLRPAELLAAVGLPPQDWQHHVRRTSVEWSF"
     gene            7579..9834
                     /locus_tag="P5316_09925"
     CDS             7579..9834
                     /locus_tag="P5316_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRR85317.1"
                     /translation="MTEHENQTQNNQTGNEHQSLPRTGRRRSRRSRRKHRPDDQQQAA
                     AVQGPPESEQVAASIVTGTVESDTASGSEAIEPVWTGSPVRHESSRVERPLDNVAASG
                     ESLAESGSSEQPVTETAPPAERRRRSRRGGRGRRRKRQNAEVAAAGQAQGTTQGSAAV
                     SETVAVKYPPRRDGPPQHTPARQQHEPEDEDEDNEQASGTGDRIMLINAADGEECRIA
                     VVHQGKLEELFIERASAESHVGNIYKGRVTNVEPSIQAAFVDFGLPKNGFLHISDLQP
                     QYFPNGRGHMEEVGRKTPRRERPPIQKCLRRGQEVLVQIIKEGIGTKGPTLTTYISIP
                     GRYLVMMPGMNKLGVSRRIEDEEARRKMRRLLDELNLPKNMGFILRTAGLDKTKRELQ
                     RDQMYLQRLWKRVAQKIKTERAPCELYQESDLVIRTIRDVYSADFAKIIVDNEATAEK
                     VRDFLAIAMPRSSAPVEVYRSPEPLFHKYGIEAEIEKINARHVPLPSGGSLVIDSTEA
                     MVAIDVNSGRFREHEDAEETAFRTDLEAAEEIARQLRLRDLGGLIVCDFIDLVQEKHR
                     RAVERALREALKKHKERAKCLRMSQFGIIEMTRQRMRPSIKRSIYQDCPHCRGSGLVK
                     NPESMTLDIMRLLRLAANHELVETVEVRVAPEVAFQLQNRKRAAIHDIEQQTGRHIVI
                     RADNNIGLDQYVFSCFDKRGGAIRALDMAEAAGSSPANRGKHSPPVQPDNQHRDRDSD
                     RDRDQDENVFE"
     gene            complement(9949..11286)
                     /locus_tag="P5316_09930"
     CDS             complement(9949..11286)
                     /locus_tag="P5316_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85318.1"
                     /translation="MIRRFDLSKLMLVCVLVPGATVSAAGAEAVEGMMITAGVTGQQA
                     APHGWSEEKVYVGQLDVGFGAGACTAHTPAGWFSIPGREPSTGFPAGTYALFTLSYDG
                     IPAFSCDMNLQIPHGAGKLQKARLETPAHYSVMYDAKGYQAWGKSPFVNGDDFYQTFV
                     ATTPYVTRIATRLADKSGDHQILNLNYAVYETNNGPPSTWKRISPVRSRHLSGTTDPI
                     IHIFHVSFRSSEMTLIPGRTYAIRLWRDSSSQSERFALVVRNDKGDGYANGHVFIGDV
                     PRRDLDAFAYISGGQPGTIVNHAPVGDPDLKQLVGSAKRHGQTFKASGIGLAGVDVIY
                     ATGDTRPPRLPFVFQTYDKPGGKPIGKAKSCYGLPLAFQGRAAAIWSKGDVPLTPGRT
                     YYIEWTSPGSNTWKLNEDLPGEAYVDGVAKPDWDLAMSIVEYADDPPATKPAS"
     gene            complement(11356..12756)
                     /locus_tag="P5316_09935"
     CDS             complement(11356..12756)
                     /locus_tag="P5316_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85319.1"
                     /translation="MKRTSRATVWLAFAALVGPSASTAADLIAGMAEVEITPPVGYRL
                     SGYFLERQSTGTHDPLKAKALVLGQRDVKAAFVFCDLIAVPRSVSEPARRLVAVRTGI
                     PADNIVITGTHTHTGPLFYGPLRKHFHEQAVAKYGKDPCEIVDYHAQLIEKIGQVVSK
                     AAESASSIVLEAATIEQDPPLSFNRRFHMKDGSVRFNPGQKNPDIVRVAGPIDPQVGI
                     VLLKAAEDRKLKACLTVFALHLDTTGGTLYSADYPFYLEKTLRASLGNDLMSFFGTGT
                     CGDVNHIDVTVEGRRKAEEIGELLAKTVIAQVPKARPVEAAPLAAKTTTVHAPKQQYT
                     QEQQAQAAKDVALVGTGKLPFLKEVEACKIVDLARYPGTTIPLEVQAFRLGGDLAIVT
                     LPGEVFVEHGLAIKKSSPFRTTLVIELANDVPAYIPTRKAFAEGSYETVNSRLQPGGG
                     EMMVEAAVEILKQLAG"
     gene            complement(12804..13439)
                     /locus_tag="P5316_09940"
     CDS             complement(12804..13439)
                     /locus_tag="P5316_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85320.1"
                     /translation="MSVPAVADYAARFKSCGRNVRIATDAFFEHPEIIEVGDNVTFMR
                     GFHMLGRPRMCQIGSNVTFYPNCFIQGSAGRFIIGDHVEFFPGAYISLGDWESSFVEI
                     GHHCHFAPNCVLYGWGGLTISPYCNIAAHAVFATVGHHDEITDKPMSLTGEKAGPITL
                     EQDVWVAANVTIAANTRIARGCVIGANAVVTKDTEPMGVYVGVPARRLRDR"
     gene            complement(13510..14685)
                     /locus_tag="P5316_09945"
     CDS             complement(13510..14685)
                     /locus_tag="P5316_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85321.1"
                     /translation="MFEVNMWTYPWDLVDEGIDRALDRLKGEAGVTGVSLAVTYHSIE
                     QLRPHPDVTPRTFRSPGGAQFQPKPELYAATRIRPVVAPWLKKSNPLAAVSEACDKLG
                     LKLRAWTVCCHGSAMVAKYPAFAMKDVFGDSSVNWLCPVNPDVREYLRALVEDLTTHY
                     LFDTVELERPSFPEKLHSHAHHKIGVVLGPVGEFLCNLCFCESCRQAAERDGVNVEAA
                     ASAAMEGLTAILSDRDFEASSRESYLSENPDLAAFVDWRSGQIASLVASLKQTSSVRV
                     VVHRVGDHYMTAADWRSIAGACDGLLAPCSYSGDDAMKTLVHEVRGDTGSMAGVELSF
                     NACSPQCPSAEAFVSAVAGAARLGVRSVDVYNYGLLPIDRLEWVRRAARYAHREHLD"
     gene            complement(15134..15535)
                     /locus_tag="P5316_09950"
     CDS             complement(15134..15535)
                     /locus_tag="P5316_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85322.1"
                     /translation="MSRLGEALRRVPAPVSYGIVCIVLVGAVVAIVMQLRPAASVTQQ
                     DAVGVVYCKCSQCGHVIEGLPVELADKGILDPVEKSTLTDSGKRCPKCGRNNALDVAF
                     KCPKDGEVFLLKRQAGRAQGCPKCGWNPYSP"
     gene            complement(15557..16333)
                     /locus_tag="P5316_09955"
     CDS             complement(15557..16333)
                     /locus_tag="P5316_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85323.1"
                     /translation="MMERHRLGFTLIEVLVVVAILALLIAVLLPSLQRAKEQAKTAVC
                     LSNLHQMGFGFSTYAADHRQNLPMRLGYTYALKYRGGPRNVRVLCNVGLLYGKYCGKD
                     LDFYYCVSNEQYAYDDPVYGAPGFFIEGRDPYVTWSGYMYAAPVAEEKSPREAGKNSY
                     PREVWRDLYAEWVESQTAAGATVGNENIKALLADNLIGCGGEKAPHKGVAFNVLFTDY
                     HAKPVRDPDRQLYRGLRRPTSGTGGAPDLYYYWDLFTKNP"
     gene            complement(16724..17104)
                     /locus_tag="P5316_09960"
     CDS             complement(16724..17104)
                     /locus_tag="P5316_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRR85324.1"
                     /translation="MHVIDIAGHNVDRHLVRDLRTLVRFIEIYCAYWHKADPRATVSL
                     KEIDVKALMGRPVTLCQGCERLLAHAVHKRSRCPINPKPACKHCPRHCYHPLFRQQIR
                     EVMKFSGRKLVLSGRLDYLWHLMR"
     gene            complement(17162..17965)
                     /locus_tag="P5316_09965"
     CDS             complement(17162..17965)
                     /locus_tag="P5316_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR85325.1"
                     /translation="MIREVVSIDEDKCNGCGLCVPACQEGAIRIVNGKAKLVADHLCD
                     GLGACLGHCPQGAITIERREADDFNEEAVAAHLGKEHQADQHTPVSVAGSCPGSQFQS
                     LAEPLPGGGCPSARLIQFGSPGAPKCPVSSATGPTASTLTHWPVKLRLLNPTAPVLQR
                     AGLLIAADCVPVAFPAFQSRLLRGRAVVIGCPKFDDIQADVDKLTAIIERNDLVEIVV
                     AHMEVPCCNGLLMATLQARRRTGRPVPIADVVIGIRGDVLSWRKLRADQ"
     gene            18092..18778
                     /locus_tag="P5316_09970"
     CDS             18092..18778
                     /locus_tag="P5316_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRR85326.1"
                     /translation="MSGDLVDIIRKCRFFGGLSDSSRRRIASLAVKKRYGKGTIIFRQ
                     GQPCPGMFVVGHGHVRVYKIAPSGKEHVLHFASEGMTFAEVAAIGGFDCPAYAEALED
                     TVCALLPQEAFLKALRSDHDLCIQLLQGMAAWVQHLIGLLEDIVLRDAAGRVARHLLQ
                     QAAGSGSEFALPILKKDLASHLNLTSETLSRILRRLAESAMIEMPDQQSIRVLDRGRL
                     AEVADGLPLG"
     gene            18845..19597
                     /locus_tag="P5316_09975"
     CDS             18845..19597
                     /locus_tag="P5316_09975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85327.1"
                     /translation="MPADIFDEHSWRYEAMVNWPKRLANEAPFYRKLFADVQASRVLD
                     AACGTGHHAEMFHSWGLSVEGADISEGMIERCRARLGTSDRLRWVVRSFDRPAEPAGV
                     FDVVLCVGNSLSLASDMDTVGRAIGAMLDSARPGGLCVIHVLNVWSLPDGPMVWQKTL
                     KTQHDGRDHLLLKAIHRSGTDTRVEVLDVMLDQPSLERWSESTPLLPITADDLISFAK
                     AAGASQVETFGDYRRTPYDRTTSPDLIAVCRK"
     gene            complement(19658..>20192)
                     /gene="corA"
                     /locus_tag="P5316_09980"
     CDS             complement(19658..>20192)
                     /gene="corA"
                     /locus_tag="P5316_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010478760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRR85328.1"
                     /translation="ARVHEIKRNLMTLRRCIWPLREAMNSLVRDPSPLVNDETRLYLR
                     DCYDHTVRVIDFIETYRELGSDLMDLYLSSVSQRMTEVMKVLTIIATIFIPLTFISSI
                     YGMNFQTDRSPWNMPELTWYFGYPFALGLMAMIALAMLYYFGRKGWLRSPGEPPRSPG
                     PPDQDRNVSDRIDNQGE"
BASE COUNT         4101 a         6094 c         6183 g         3814 t
ORIGIN      
        1 tgtttgcagc cttctggtct gccggagcgg ttaccggacc gggcatcagc ctttcgacct
       61 ccttgactcg atcgccgtat ctgcgttcga tgtcccggcg agccagggcg tgcatctcca
      121 gcggcttatc accgactttc atcacgacgc gcacaatctg gccgtcgtca tcaacataga
      181 tttcatgaac cggcggaata agcccctcgc tttgctcgat cagcgtggct tccttgccgc
      241 ccggcatggt cttgcgaccg acgagccgga aggtctgcaa tgacaaccct cgccgggagc
      301 tgtcgaacca tgagaaagcg tacagctcgg tcttcttgag atcgacaatc gacggaaaga
      361 gcaggtccca gggagccgat gcgtaggtct gatcgacatc gagttctttc tgcgtcatct
      421 gcggcccgaa ggatttgcct gcatagccga caagcaattt ggtctcctgc cgtatgccgc
      481 tttcgatgtc ctgcgagatc tccctgcgtc caccgccctt gggtggcggc gagatcgcga
      541 tcaaccggct ctcccatcgt tcgaacgaca gattagaggt gacgaacata tcctgctgca
      601 tgagggcaat gctttgatcg ggtcggaaga accacgccca cttcgccacg gtgacgcctt
      661 tccggtgttc gaccgttcgc ggctccatac gaatctgcat gaatccgatc tcgactccgc
      721 cctcaagaca tcgcaggaac acttcccgcg gctcgcggcc gaccacgtcc agtgccccgg
      781 accgcaccag atcgagcaac gcgctgcccc ttcgcaaggc gtcccgaatc tgctcctctt
      841 gagcctcaga cctcagtacc tcgaaacctg tgacaatctt gtcgaaaaca tcgatggccg
      901 ctttctcgtc ggactgcggg gccgcaaaga ccagcgtgaa gtattcggag ggcttgacgc
      961 gaatcatggc ctgcaagcga atcatcggaa cgccgcgtgc ggtcatcagc gccttgcagc
     1021 gcaggatctc gcgagatccg acggtcgatc tgtcggtttt cgcgacggtg atgtcgggat
     1081 aggtctcctg catttcgccg atgaccatct cggcgagcgc ttccggatcg agcgcccgct
     1141 cgggcttgct ctgacgaacg cccagcgacc agcggccgtt gacgtccacg aagcggacga
     1201 tctccacttc gttgatcgtc tccatgaatc gcttttcgcg gagtgtctcg cagccggtcg
     1261 ggggggttat cgcaaacccg aagcttccat ccacgtatcg cgccccaagc gtcgcggcga
     1321 atgcgggagg tgaatcggcg gcatcctggg cgagaacaac cgacaggctg cccgacagta
     1381 aaaccgccat caccaaccct gccgcacagc caaagctaac gttctttcgt agccccatca
     1441 ttgcagcact tctcgcatcc tggccccgat caaggacaat taccggcgca aacctggccc
     1501 gacgcccggc tgttcctcgg cttcagaagg cggctgcacg cgcttgcgcc attgccttgc
     1561 gtcgtgggac acggcaagca gctcgtcgcc gtcgtcgaaa tacatcaaca tcggctccgg
     1621 atcgtaatcc atcatcaatc gtacagtccg cgccggctcc cgcatgggcg tgcccggcac
     1681 gggtgtcctg cccagaggct tgatcacaca gtaggccacg ccagccgtaa acgtgtcaat
     1741 ggtactgacg accaccgatc tctggctcga gctgccggcc gtccttgcgc acagctccgt
     1801 aaggagcggc cccgaggcaa aggcatcatc cacctcgatc tcctcggttc gcgatccgcc
     1861 gaatgctttg acaatccgag tcacctgccg accttcggcg tcggagtgtt cagtctgctc
     1921 aatactcttt tctccggcgt cgctgcgctc gaacctgtac aggtattcga cgccgttttc
     1981 cgccagacgc catccctccg tcaccactat gcgagcgtgt ccgcgccacc ggggatcata
     2041 aacccaacgg cccgtgacct cccacggcag ctcggcattg tcggaagacc ggcggcaggt
     2101 ttcgatccaa ctgcccagac cgagcgccgg ggcctcgacg ttgaaacgca gcctcctgcc
     2161 ctcgcgacgt cccagcaggg atgacatacc ctccttgcgg caatccttga tcaaccttcg
     2221 ggcagcatcg atggccgatt ccagatcgac cagctcgtcg taagattcaa cccgagccgt
     2281 ggccgcgatt gaatcacaca gctctcggac ggcagttccg gcttccgcgt catgccgtcc
     2341 caccatcagg agggccgtat gctgatcggt cttgacggcg accaagtgga ccgtcggctc
     2401 cttccccgga gtgatcacaa gactggcacg cgccgcctgg cgcccgcccg gatcgaaagg
     2461 ctccagcggt tcacccagtc tcacctgctg aagaagcgac atggccccgt cctccaccag
     2521 gtcgttgacc ttccgacggg cgatcagcgg gacgcggaac agatcgaggt accaagcagg
     2581 tgccgtttgc ctgctcatga ggcgcacgcg agggagcccc cgctcgattg gaccgataaa
     2641 tcgagctccg cgtgggatgt cgaagctgat acccgcggcg gccatagctt ccgcagggtc
     2701 cgggtcgagc ccaatcgatg tcagttcgac ggtctcggcc actcgttgga cgagaagctg
     2761 atccgtcctt cgcgacggca aggcggtttc gagcaacacg ccgacgacct ctccccccgg
     2821 cccgacagcg gcccgtccaa ggcaaaaggg tggcttgacg cccaccgcgc ccagcctctt
     2881 ggccatggtc gcagtcaccc cggggaatgc ccccacttgt gccgtctcag gctccttctc
     2941 aaacagaaca tcggccccca aagcgtttga gaatgagtca agggcccgga ggctggccga
     3001 cgcggctaaa ccctcagagg agggtggacc tcgaaaaacg taaagtcgcc tctgacgccc
     3061 ttccgtctct cgatcttcga cggcgatcac agtgccagcg gggcaaagag ccttatcgag
     3121 ggtctgccat cccaacggca aagatacgga taattgcccc tcggggtaat gaacactgga
     3181 cccgagctcg atcagctttg ttttttcccg gtgccagatg aggagttggg cagcccctgc
     3241 ggccagcagg agcagcgcaa tggcagccac gagccttctc aggggctgtc gggggcgctg
     3301 agcggaatgc acttcgtaca tgcgtcggca tcttagcgct tgcggtcaac cattgcaaac
     3361 aaagggcttc catcgagcgt aagcggtatt gacgcaggcc ggagcatggc ttacattcgc
     3421 aaaagcgatg cctctgccaa ggtgttattc agccaccgct gcggctcggg ccggggaagg
     3481 ggtcagcccg tacccggtac ccggggccct gttcactgga cggggaaggt ccgccacgct
     3541 gacgcgaagt cccctttccg ctggcacggt cgcccgcccg cacaacttgg cagcctgccc
     3601 ggacattgtg gagcattggc gatattggac aaaggaggtt cacctgtgca gatctccgta
     3661 acgggccgcc acatagagtt cactgaatcc atgcggcagt acgcggaaga aaaactgagc
     3721 aagttggcaa ggttctatga tcggatagac agggttgagg ttgtgctcga acaggagccg
     3781 acgaaacatc ggttggaagt ggtgatgcat gcggatcaca agacgacttt cgtcgcccag
     3841 gttgaagccg gggagtacca cgaagccgtc gatctgatcg tggaaaagct ggaacggcag
     3901 cttaagaagc ataaggagat atatcgcaac cgcaagcacc agtcctgagc aggggccggg
     3961 ccggcgggcg ttcgggttcg atacccgagc cgacgcatat accccccaag gtgtacaaac
     4021 atgaaactgt cggatttttt tgtaccggaa gcggtcattc cggaattgaa ggcgacggac
     4081 cgcaacggcg tgattcgcga attgacggct tcgctggccg cagcggtcgg catggacgaa
     4141 gcccaggccg cggcgatcag caaggccgtt attgcccgtg agaatcaggg cagtacaggt
     4201 ttcgggaaag gggtcgcggt tcctcacgtg aaacacccgg ccgtcaagag aatcatggcg
     4261 accctgggcc gctcaagcac cggcgttgat tttgcggcgc tggatcgggc acccgtgtat
     4321 attgtcgtgc ttttgctgag ccccccggac aatccggatc agcatctggc atcgatggag
     4381 aacattttct ggcacctcca aagggacaac ttccggcgtt ttctgcggca ggcgtccacg
     4441 aaggaggaaa tcaacgagaa cattcgggag gcggatgagc ttcccggcgg ataagcggat
     4501 cgatgtccga gcatcgactt gccgcgacgg caaacggcgc atgatccggc ggctttttat
     4561 gaacgagacg gctgtgcccg aagcatctcg acgcgtgacg atcgagaatt ctcttgggtt
     4621 gcacatgcga cccacgatgc agttcgtcga tctggcgaat cagttccggt cgcgtgtcgt
     4681 cgttcggaag ggtaagcacg cggtcgacgg caagaacccg atggagatga tgcttctgga
     4741 ggccgtgcgc ggcaccgagc ttgagattca agcggcgggg gccgatgcgg aggacgcggt
     4801 ggaggcgctg gccgctctgg tgaaatcggg atttggggaa cagtgaccga ggccgcgccg
     4861 ccttaggttg atcgaacaag gttgagaacg tgtcgaccga gaatagatga ccatggagat
     4921 caagaaggga atcggggtct ctccaggcgt cacgatcgcc aaggcggtgg tgctggacgc
     4981 cgaggagtac cggattgccc agaaaacggt ctcggtggat gacattccgg ccgaactgga
     5041 gcggttgagg agcggtttcg acgcgtcgct ggccgagttg cacagtctcc aggaagtcat
     5101 ggcccggcag ttagggcctg acatcgcggc cattttcgat tttcattacg gcgtgctcag
     5161 tcaagaccgt ttgttcgagc agatcgccgc gttgatcacc cagaagaact actcggccgc
     5221 ctacgcggtt cgtgaggtgc ttcgcggcta ccagcggaga ttcctgaaca tgaaggacca
     5281 gctcctgcgc gagcgggtcc gggacgtcaa cgacattgaa cggcggctgc tgaggcacct
     5341 ttgcggccag agcggcgagg aactgagcct tctgaccgag cctttcgtac tcgtcgccca
     5401 cgacttgacc ccatcgcagg tcgccaactt aagccagacc aaggtgagcg gcgttgcgat
     5461 ggatgccggc ggcctgacca gtcatgcggc gatcgtggtt cgatcgatgg gcatccctgc
     5521 ggtcatgggg ctcaaggacg tatcgacgcg ggtgtccaag agcgacacga tcattctgga
     5581 cggcacgaag ggactggtgg tcgttcggcc cgacgcggac atgcttcagg aataccgggt
     5641 cgaggaggag cgtatccacg ccctggccag cgacctgatc gagctgcgcg acaaaccggc
     5701 ggtcaccacc gacaacatca agatcacgct tctgaccaac atcgagtttc cctacgaagc
     5761 caagacctgc ctggaaaagg gtgccgaagg tatcggcttg tatcggacgg aattcctcta
     5821 cctgcggagt gaggccgagc ccacggagga ggagcattac gaagcctatc acaccgtgat
     5881 tcaggcggtc ggcgattacc cggtcaccat ccgcacactg gatttggggg ccgacaagta
     5941 cacgcagtca caggctcgcg agcccgagcg aaatcctttc ctcggcttga ggtcgatccg
     6001 ctactgtctg caaaaccttg atctgttcaa gacccagctc cgggcgatcc ttcgtgcttc
     6061 ggtggagggt gacgtgagga tcatgtttcc gctgatttcg agtctgatgg agcttcggca
     6121 ggcgaagatc gtccttggag atgccatgga agacctggag gaggccggta tccccttcag
     6181 gcgggacatc ccggttggta taatggtaga gacgcccgcg gcagccttgc agatcaagga
     6241 attgctgaga gaggtggact ttatcagtat cgggaccaac gatctgatcc agtacaccct
     6301 ggcggtcgac cgaagcaatg aacgggtggc ctcgctgtat acggccagcc atccggtcgt
     6361 cctgaggatg ctgcgggaca ttatgcgggc cgcggagagg gccggaattc cttatagcat
     6421 gtgcggcgaa atggccgggg agccgatgta tacgttgttg ctgctgggca tcggactgcg
     6481 gcagttctcg atggccccga gcgacatccc ggagatcaag aagattatcc ggtccgcttc
     6541 gattgcccac gcggtccgcg tggcgaagcg ggcgctctcc ttcgatactg atcggcaggt
     6601 aagcaactac ctgcgggatg agacgaggaa gatctggcct gaattcatgt aaatgagtcc
     6661 ggccgcacaa cgagacgctg agccacgcct tggggtgtgg ttgcacaata cgcaggccct
     6721 tgatcgaccg ccgtaagtcg gtcaacggca agccggctcg cgcgttacga tgccggcgca
     6781 gccaagagac ttttggagtc agtaatcaag acgaggacat agcaaacgag ttgcagtaca
     6841 gagtggcgat ccgcttcgtg atcgacgggg acattcggtt catctcgcat cacgatacca
     6901 tgcgtctgtt tgaacgggcg ctgtcgcggg cacaattgcc ggtgagattc tcgcgcggct
     6961 tcaatccgcg ccccaggttg tcgctgccgc tgccgcgagc cgtgggcatc gccggcgcgg
     7021 ccgagttact ggtggtggac ctggaagagc cgttggatcc ggcggtggtg cttgacaagc
     7081 tccgcgaaca aatgccggcc gggttgactc tgagcgaagc ccggtcgatc gccggcaaac
     7141 atccgcccca agcagtgaga gccgattacg cgttggccct ccccccggat cgggcgaagg
     7201 aagtgggcca gagaatgaaa gatcttctgg cggcttcaat gtggaatatc cagcgatccg
     7261 gacccgggac gaaacggcct agggaactcg acgtacggca gtacctggcg ggcggcgcgg
     7321 tttgtgaggg gattctgcga tggacggtgc gggtcaccgg ggccgggtcg ctgcgaccgg
     7381 ccgagttgct ggccgccgtc ggcttgccgc ctcaggactg gcagcatcac gttcgcagaa
     7441 ccagcgtcga gtggtcgttc tagtgcagcc gtcatccggc cgccggcacg tccgtccacg
     7501 accggcagat gcagacaatc gccttccact cagtacctgc cgctcctact gacagggacg
     7561 tcgtgagagc aggaaacaat gactgaacac gaaaaccaga cacagaacaa tcagaccggg
     7621 aacgaacatc aatcattacc acgaaccgga cggcgccgca gccgtcgatc gcgccgcaaa
     7681 caccggccgg acgatcagca acaagccgca gccgtacagg ggccgccgga aagcgaacaa
     7741 gtcgcggctt ccatcgtcac cggcacggtg gagtcggaca cggcatcggg ttccgaagca
     7801 atcgagcccg tatggacggg atcgccggtc cggcacgagt catccagggt cgaacgtccg
     7861 ctggacaacg tcgcggcgtc cggcgaatca cttgcagaaa gcggctcaag tgaacaacct
     7921 gtgacggaaa cggcaccgcc tgccgagcgt cgccggcgat cgcgccgcgg cgggcgcggc
     7981 cgacgccgca agcggcagaa tgcagaggtt gccgcagccg gccaagcgca aggcacaacg
     8041 cagggatcag cagcggtttc ggagactgtc gccgtcaagt atccgcccag gcgagacggc
     8101 ccgccccagc acaccccagc ccggcaacag cacgaaccgg aggatgaaga tgaggacaac
     8161 gaacaggctt cgggaaccgg cgatcgcatc atgctgatca atgccgcaga cggcgaggaa
     8221 tgccgcatcg cggtcgtgca ccaaggcaaa ctcgaggaac tgtttatcga gcgggccagt
     8281 gccgagtcgc acgtgggcaa catctacaag ggccgcgtca cgaacgttga gccgagcatc
     8341 caggcggcgt tcgtggactt cggactaccc aagaacggat ttcttcacat ctctgatctg
     8401 cagccccagt actttcccaa cgggcgagga cacatggagg aagtgggacg caaaaccccc
     8461 cggcgagagc gaccgccgat ccagaagtgc ttgcgacggg ggcaggaagt cctcgtgcag
     8521 atcatcaagg aaggcattgg caccaagggg ccgacgctca cgacctacat ttccatcccg
     8581 ggccgttacc tggtcatgat gccgggaatg aacaagctcg gcgtctcccg ccggatcgag
     8641 gatgaagagg cacggcgcaa gatgcgacgg cttcttgacg aactcaatct gcccaagaac
     8701 atgggcttta ttctgcggac agccggtctc gacaagacca aacgcgagct tcagcgagat
     8761 cagatgtacc tgcagcggct ctggaagcgc gtcgcccaga agatcaagac cgagcgagcg
     8821 ccctgtgagc tttaccaaga atcagacctg gtgatccgca cgatccgcga cgtgtactcc
     8881 gcggattttg cgaagatcat cgtggataac gaggccacgg cggaaaaagt gcgcgacttt
     8941 ctggccatcg ccatgccgcg cagcagcgcc ccggtcgagg tttaccggag ccccgagccg
     9001 ctcttccaca agtacggcat cgaggcggaa atcgagaaga tcaatgcccg tcacgttccg
     9061 ctgccgtcgg gcgggtcgct ggtgatcgac tcaaccgagg cgatggtcgc gattgacgtc
     9121 aacagcgggc ggttccgcga acacgaggac gccgaggaaa ccgctttccg gacggacttg
     9181 gaggcggccg aggagattgc gcgacagttg cggcttcgag accttggcgg actgatcgtt
     9241 tgcgatttca tcgatctggt gcaagaaaag caccgtcggg cggtggaacg ggctcttcgg
     9301 gaagccctga agaaacacaa ggagcgggcc aagtgtctgc gtatgagcca gttcggcatc
     9361 atcgagatga cccggcagcg tatgcggccg tcgatcaagc ggagcatcta ccaggattgc
     9421 ccgcactgcc gcgggtcagg tctggtcaag aatccggaat cgatgaccct tgatatcatg
     9481 aggctgctgc gtctggcggc aaaccatgaa ctcgttgaga cggtcgaggt ccgggtggcg
     9541 cccgaggttg cctttcaact ccagaaccgc aaacgggccg ccatccacga catcgaacag
     9601 cagaccggcc gacacattgt catccgcgcg gacaacaaca tcggacttga tcagtacgtt
     9661 ttctcctgct tcgacaagag agggggcgcc attcgggcac tggacatggc ggaggcggcc
     9721 ggctcatcgc ccgcaaaccg cggaaagcat tcgcccccgg tccagcctga taaccagcat
     9781 cgcgaccgcg actcggatcg ggatcgggac caggatgaga acgtctttga atagggactt
     9841 cgccgtctgg gacaatggtt cgcccgaaag gttgcgacga cgagccggat acttgagacc
     9901 aacaagtgat gtgaccttgc cgtccggcgg ttcgatcact tggtccgatc aactcgccgg
     9961 cttcgtcgcg ggtgggtcgt cggcatattc gacgatggac atagccaagt cccaatcggg
    10021 cttcgcaacg ccgtcaacat aagcttcgcc gggcaaatcc tcgttcagct tccaggtgtt
    10081 cgaaccgggg gaagtccatt cgatatagta cgtcctcccg ggcgtcagcg gcacatcacc
    10141 cttggaccag atcgccgccg cccgaccctg gaaagccaac ggcaacccgt agcaggactt
    10201 ggccttgccg atcggtttac cgccgggctt atcgtaagtt tgaaagacga agggcagtcg
    10261 cggcgggcga gtgtcacccg tggcgtagat cacgtccact ccggccaagc cgatcccgct
    10321 cgctttgaac gtctggccat gacgcttggc actgccaaca agttgcttga gatcggggtc
    10381 ccctaccggc gcgtggttga cgatcgttcc gggctgccca ccgctgatgt aggcgaatgc
    10441 atcgagatct ctccgcggca catcacctat gaaaacatgc ccattcgcgt agccgtcgcc
    10501 cttatcgttc cgtaccacca gagcgaagcg ttcggactgc gagctggagt ctcgccaaag
    10561 tctgatggcg taggtccgac cggggatgag agtcatttca ctgctgcgga atgagacatg
    10621 aaagatatgg atgatcgggt cggtggtgcc cgacagatgg cggctacgaa cgggtgagat
    10681 tctcttccag gtcgaaggcg ggccgttgtt ggtttcgtac acggcgtaat tgaggttgag
    10741 aatctggtga tcgcccgact tgtcggccag cctggtcgcg atccgggtga cgtagggggt
    10801 cgtcgccacg aatgtctggt agaaatcgtc gccgttaaca aaaggtgatt tgccccacgc
    10861 ttggtatccc ttggcgtcgt acatgaccga gtaatgagcg ggggtttcga gcctggcttt
    10921 ctgcagcttg cctgcgccat gggggatctg caaattcatg tcacaactga aggcgggaat
    10981 gccgtcgtag gaaagcgtga acagtgcata ggttccggcc gggaagccgg ttgatggttc
    11041 cctgccggga atcgagaacc atccggccgg cgtatgcgcg gtgcaggctc ccgcgccgaa
    11101 gccgacgtcg agttggccga cgtagacctt ttcttcgctc cagccgtgcg gggcagcttg
    11161 ttgcccggtg accccggccg tgatcatcat cccttccacg gcctcggccc ccgccgcaga
    11221 gaccgtcgcc cctggaacga gaacacaaac caacatcagc ttggacaagt caaatcgtct
    11281 tatcatgagt cactccacat caagtccggg ccgggaacag acgcccaggg gatagcctag
    11341 ctcctttacg tgagactacc cagccaattg cttcaggatc tcgacggccg cctcgaccat
    11401 catctcgccc ccgccgggct ggaggcgtga gtttaccgtc tcatagctgc cctcggcaaa
    11461 agccttacga gtgggaatat aggcgggaac gtcattggcc agttcgatca ccagggttgt
    11521 ccggaacggc gaggacttct tgatcgccag accgtgctcg acgaacacct cgccaggcaa
    11581 ggtcactatc gccagatcgc ctccgagccg aaatgcctgc acctcaagcg gtatcgtcgt
    11641 gcccgggtac cgagccagat cgacgatctt gcaggcttcg acctccttca gaaatggcag
    11701 cttgccggtg ccgaccaggg ccacgtcctt ggcggcctgg gcttgttgct cttgggtgta
    11761 ctgttgtttc ggagcgtgta ccgtcgtggt tttcgctgcc agtggtgccg cttcgaccgg
    11821 ccgtgccttg ggcacctgag cgatcacggt tttagccagc aactcaccga tctcctcggc
    11881 cttccgccgc ccttcgaccg ttacgtcgat atggttcacg tcgccgcaag tgccggtgcc
    11941 gaaaaaggac atcagatcgt tgcccaacga ggcacgcaac gtcttctcga ggtagaaggg
    12001 ataatcggct gaatagagcg tgccaccggt ggtgtcgagg tgcaaagcga aaaccgtcag
    12061 gcaggccttg agcttccggt cctcggccgc cttgagcagc acgatgccga cctgggggtc
    12121 gatcggcccg gccactcgga cgatgtccgg gttcttctgc ccggggttga aacgcacgct
    12181 gccatctttc atatggaatc ggcggttgaa agacaggggc ggatcctgct cgatcgtcgc
    12241 cgcttcgaga acgatcgatg aggctgattc cgcggccttt gaaaccacct gtccgatctt
    12301 ctcaatcagt tgggcgtggt aatcaacgat ttcgcatgga tctttgccat atttggccac
    12361 cgcctgctcg tggaagtgtt ttcgcaaggg tccgtagaac agcggaccgg tgtgtgtatg
    12421 ggtgccggta atgacgatgt tgtcggccgg gattcctgtt ctgacggcca cgagcctgcg
    12481 ggccggctct gagacgcttc ggggcacggc gatcaggtcg cagaaaacga acgccgcctt
    12541 gacatctcgc tgacccaata ccagagcctt tgccttgagc ggatcatgcg taccagtgct
    12601 ctgcctttcg aggaagtaac cgctgagccg atagccaacc ggcggtgtga tctccacctc
    12661 ggccatgccg gcgatcaagt cagcagcggt acttgccgac ggaccgacca aagccgcgaa
    12721 tgccagccaa acggttgctc ttgaggtgcg tttcatggtt atgctcctgc cgatcatcgc
    12781 cgcctcacgc gcaggggggg ccatcagcgg tcccgcaacc gcctggctgg cacgccgacg
    12841 tacacaccca tcggttcggt atccttcgtc acgaccgcgt tggcgccgat gacgcagccc
    12901 cgggcgattc gggtgttggc agcaatggtc acgttggcgg caacccagac gtcttgttcg
    12961 agcgtgatcg gtccggcttt ctcgcccgtc agcgacatgg gcttatcggt gatctcgtcg
    13021 tggtggccga cggtcgcgaa gaccgcgtga gccgcgatgt tgcagtacgg cgagatggtc
    13081 agcccgcccc agccgtagag cacgcagttc ggggcgaaat ggcagtggtg cccgatctcg
    13141 acgaagctcg attcccagtc accgagcgag atgtaggctc ccggaaagaa ctccacgtgg
    13201 tcgccgatga tgaaccgccc ggccgagccc tggatgaagc agttggggta gaaggtcacg
    13261 tttgacccga tttggcacat ccgcggccgt ccgagcatat ggaagcctcg catgaaggtc
    13321 acgttgtccc cgacctctat gatctcgggg tgctcgaaga aggcatctgt cgcgatgcga
    13381 acgttccggc cacacgactt aaagcgagct gcgtagtccg cgacagcggg aacactcatg
    13441 tttgtcacac tccgggcgac tctgcctgct cccgaacgcc ccattgccta accggggtgc
    13501 cgacggctgt caatcgagat gctcgcgatg ggcgtaacgt gccgctcggc gaacccactc
    13561 cagccgatcg atgggtagca ggccgtagtt gtagacgtca accgaccgga cacccagcct
    13621 ggcagcgccg gcgacggcgc tgacgaaggc ctcggcgctg ggacattgcg gggagcacgc
    13681 attgaatgaa agctcgacac cggccatgct gccggtgtcg ccgcggacct cgtgcaccag
    13741 cgttttcatc gcatcgtcgc ccgagtagga gcacggagcc aggaggccgt cgcaggcacc
    13801 ggcgatcgac cgccaatccg cggctgtcat gtagtggtcg ccgacgcgat ggacgacgac
    13861 acgaacggat gaggtctgtt tgagagaggc gacaagcgat gcaatctgcc cgctccgcca
    13921 gtcgacgaac gccgccaaat cagggttttc agacaagtac gattctcgcg aagaagcctc
    13981 gaaatctcga tcggagagga tcgcggtgag gccttccatg gccgccgaag ctgccgcctc
    14041 aacgttgacg ccatctcgct cggccgcttg acggcaggat tcgcagaaac agaggttgca
    14101 gaggaactcc cccaccggcc cgagaacgac accaatcttg tgatgcgcgt gggaatggag
    14161 cttctcggga aacgacggtc gctcaagctc gactgtgtcg aacaggtaat gcgtggtcag
    14221 atcctccacc agcgcgcgca ggtactcgcg aacatccgga ttgaccgggc aaagccagtt
    14281 gaccgaggaa tccccgaaaa cgtccttcat ggcaaaggcc gggtacttgg cgaccatggc
    14341 cgaaccgtgg cagcagacgg tccaggcccg cagcttgagt ccgagcttgt cacaggcctc
    14401 ggaaacggcc gccagtggat tgctcttttt cagccagggc gccaccaccg gccggattcg
    14461 ggtcgcggca tacaactccg gtttgggttg gaactgagcc ccgccgggcg agcggaaggt
    14521 tcgaggggtc acatccggat gcggccggag ttgctcgatg ctatggtaag tcaccgccag
    14581 cgaaaccccg gtcacgccgg cttcgccctt gagccgatcg agagcccggt cgatgccctc
    14641 atccaccaag tcccacggat aggtccacat attgacttca aacatgacgt aatcctgcct
    14701 gtggttcgtc cccggcaaca acgaagcgtc gtctagtcta tgaaggaata gtagcgtagg
    14761 ctggaagagc ctaccagatc gcccaccggg atgcgtgcga ccgggtctga gatcacgacc
    14821 cgggtcctgg atctcgggct gaaagacacg gaggcggcag atatccgcac acgcccagac
    14881 cggcaaagga tttgcctggc ggatcgggga cgagataatc atccggcgag cgacatcaaa
    14941 ccgaccatca gccgagggtc gagggccatc ctaaggcgga acgacttttt gcgggccgga
    15001 acccaagcgc tctgcgatca agaaacatgc gcgcaagccg cacagatgtc tggccgtaag
    15061 gtaccatgaa ggaggcgagc cgcccaatct gcacgtccat acctcgatgg ggctaaatcg
    15121 tccgaaggtg ggcttatggt gagtacggat tccaaccgca ttttgggcat ccctgcgcac
    15181 ggccagcttg tcgcttaaga agaaacacct cgccgtcctt gggacacttg aaggcaacgt
    15241 ccagcgcgtt gttgcggcca cacttggggc agcgtttgcc gctgtcggtc agggttgatt
    15301 tctcgaccgg atccagaatg cccttgtctg ccagttcgac gggcaacccc tcaatcacgt
    15361 gcccgcattg ggaacacttg cagtagacga ccccaacggc gtcttgttgc gtaaccgagg
    15421 cggccgggcg caactgcatc acaatggcaa ccacggcccc aaccaacacg atacagacga
    15481 tgccgtacga gacgggcgcc ggcacgcggc gcaatgcctc acccaatctg ctcatgatca
    15541 gcgtccgcct tgcgcatcag gggttcttgg tgaagagatc ccaatagtaa taaaggtccg
    15601 gagctccccc ggttccgctt gtcggacgcc gcaggccacg atagagttgc ctgtcgggat
    15661 cgcgcaccgg cttggcatgg taatcggtaa acagcacatt gaacgcaacg cccttgtgtg
    15721 gcgccttctc acctccacag ccaataagat tgtcagcaag cagcgccttg atattctcat
    15781 tgccaacggt cgccccggcc gcagtctggc tctcgaccca ttccgcgtac agatcccgcc
    15841 acacctcgcg aggataggag ttcttgcctg cttctctggg cgacttttcc tctgcgacgg
    15901 gcgccgcgta catgtaacca ctccaggtga catacggatc tctgccttcg atgaagaaac
    15961 ccggcgcgcc gtaaaccgga tcgtcgtagg cgtactgctc gttactgaca cagtagtaga
    16021 aatctaaatc cttcccacag tacttcccat acaaaagccc tacgttgcac agaacccgaa
    16081 cgtttcttgg ccctcccctg tacttaagcg cataggtata gccaaggcgc atcgggagat
    16141 tctggcggtg atctgcggca tacgtggaga atccaaaccc catctggtga agattcgaca
    16201 gacacaccgc cgtcttggcc tgttcttttg cccgctgaag cgaagggagc agaactgcaa
    16261 tcagcaaggc caagatcgcc accacgacca gaacttcgat gagggtgaag cccagcctgt
    16321 gacgctccat catcctaagg cgagccgcct ttttgcgggc ggcaacccaa acgttctgcg
    16381 atccagagac atgcgcgcaa gccgcacaga tgtctggccg taaggtttta gcgccggttc
    16441 gtcgcgagaa tcgcatgcgg tcgctcctat ctcaccatac caattgttgc cctgcgccat
    16501 ctgccccctg ttttgcctcg cctaccttgt ctacactatt ttcgtcattt tgtcaacggt
    16561 tgtttggcct gattcacttg tgccaaccga aatctctgtt acgcctggat ttgaggccag
    16621 ggatgccggg cgtgcagctc aggattgtta ttcatattca gcatgcaatt caatccttgc
    16681 tttgaggcct gtagatcggg atcccgtgcc ccacgcccaa tgctcacctc atcagatgcc
    16741 agaggtagtc caagcggccg gacaagacca gcttccggcc ggaaaacttc atcacttcgc
    16801 gaatctgttg tcggaacagg ggatgatagc agtgtcgggg gcagtgcttg caggccggct
    16861 tgggattgat cgggcaacgt gagcgtttgt gaacagcgtg ggcgagcagt ctttcgcatc
    16921 cctggcacag tgttacgggt cggcccatca aggccttgac gtcgatctct ttgagcgaga
    16981 ctgtggcgcg tggatcggct ttgtgccagt aggcacagta gatttcgatg aaacgcacga
    17041 gcgtccgcag atctcgcacc agatgccgat caacgttgtg ccctgcgata tcgatcacat
    17101 gcatgttccg cgtctccggc ccgcagccac tttataggca cggccggcca acgcggccac
    17161 tttattgatc tgcccgaagc ttccgccacg agagcacgtc gcctcggatg ccgatcacca
    17221 cgtccgctat cgggacaggc cggcccgtcc gccgccgagc ctgaagcgtc gccatgagta
    17281 gtccgttgca gcacggaacc tccatatgcg cgactacaat ctcgaccaga tcgtttcgct
    17341 caatgatggc agtgagcttg tcaacgtcgg cctgtatgtc gtcgaacttt gggcatccga
    17401 tgactacagc acgcccacgc agcagtctcg attggaaggc gggaaaagcc acagggacgc
    17461 aatcggctgc gatcagcagg ccggccctct gcaacactgg agccgttgga ttcagaaggc
    17521 gcagcttcac cggccagtga gtcagcgttg aggcggttgg gcccgttgcg gaggaaactg
    17581 ggcactttgg ggcgcccggc gagccgaact ggatgagccg cgccgacggg cagccgccgc
    17641 ccggcagcgg ctctgccaga gactggaact gagaacctgg acaacttccg gccactgaga
    17701 ctggagtgtg ctggtcagcc tgatgctcct tgcccagatg ggccgcgacg gcctcctcgt
    17761 tgaagtcatc ggcctcgcgg cgctcgatgg tgatcgcgcc ttgtggacaa tgtcccagac
    17821 acgcgccgag cccgtcacac aggtggtcgg cgacgagttt cgccttgccg ttgacgatgc
    17881 ggatggcacc ctcctggcag gcgggaacgc acagaccaca gccattgcac ttatcctcgt
    17941 cgatgctcac gacttcgcga atcatggggc gtcctttcaa agagccgccg acgttctatc
    18001 cgccgagttg attctgtcct aacatatggc tttacaggcc gccggaatcc ttgacctggg
    18061 tcaaggcggc gcaattttgt tgtgcgaagc catgagcgga gacctcgtcg acattatcag
    18121 gaagtgccga ttcttcggcg gtctttctga ctccagtcgc cggagaatcg caagtctggc
    18181 tgtgaaaaag cgttacggga agggcacgat catctttcgt cagggtcaac cgtgcccggg
    18241 aatgtttgtc gtaggccacg ggcatgtccg tgtgtacaag atcgccccga gcggcaaaga
    18301 gcatgttctg cattttgcgt cggaaggcat gaccttcgcg gaggtggccg cgatcggagg
    18361 attcgactgc ccggcgtatg ctgaggcctt ggaggacacg gtctgcgccc tgttgcccca
    18421 ggaggcgttt ctgaaggcgc tgcgatccga tcacgatttg tgcatccaac tgctccaggg
    18481 aatggcggct tgggtgcagc acttgatcgg tcttctggag gacatcgttc tgcgggatgc
    18541 ggccgggcgc gtggcgcgac atctgctgca acaggcagcg ggcagcggat ccgagttcgc
    18601 tctaccgatc ctgaagaaag acctagccag ccacctgaat ctgaccagcg agaccttgtc
    18661 gcgtatcctt cgacggctgg ctgagagcgc tatgattgag atgccggacc agcagagtat
    18721 ccgggtgctc gaccggggaa ggctggccga agtggcggac ggtctgcctc tcgggtagtg
    18781 cacctgagga ggtgtcccga gcagggaccg agcggcgttg aggtgaccac ggggagacga
    18841 ccgtatgccg gcagacatat tcgacgagca ctcatggcga tatgaagcca tggtgaactg
    18901 gccgaaaagg cttgccaatg aagccccctt ctatcgcaag ctgtttgcgg atgtccaggc
    18961 gagccgcgtt ctggacgcag cttgcggcac gggtcatcac gccgagatgt ttcattcgtg
    19021 ggggctttcg gtcgagggcg ccgacatcag cgaagggatg atcgaacgtt gccgggcacg
    19081 gctgggcacc tcggatcggc ttcgatgggt ggtacggtcg ttcgaccggc ccgccgagcc
    19141 ggcgggtgtt tttgacgtgg tgctgtgtgt gggcaattcg ctgtccctgg cgtcggacat
    19201 ggataccgtt ggccgggcca ttggggcgat gcttgactct gcccggcccg gcggcctctg
    19261 tgtcatccat gtgctcaatg tgtggtccct gcccgacggg ccgatggtct ggcagaagac
    19321 gctcaagacg cagcacgacg gccgggatca cctcctgctc aaggccatcc accgttcggg
    19381 cacggacacg cgcgtcgaag tgctcgacgt gatgctggac cagccaagcc tggaacggtg
    19441 gtccgaatcg acccccctgc tgcccatcac tgccgacgat ctcatctcat ttgcgaaggc
    19501 cgccggcgcc tcgcaagtcg agacgttcgg cgactatcgg cgcacgccct acgatcgcac
    19561 gaccagcccg gacctcattg cggtatgccg caagtgacct ggaaaggcat gacttacatg
    19621 gtcgacaggg gcccttgcgc gccctatcga gggcatgtta ctcgccctga ttatcaattc
    19681 gatcagagac attccggtct tgatccggtg ggccgggcga gcgcggcggt tcgccgggtg
    19741 atctcagcca gcccttgcgg ccgaagtaat aaagcatggc aagagcgatc atcgccatga
    19801 ggcccaaggc aaaggggtag ccgaaatacc aagtcaattc cggcatgttc cacggcgacc
    19861 ggtcagtctg gaagttcatc ccatagatgc ttgagatgaa cgtcaagggg atgaagatcg
    19921 tggcgatgat ggtgaggact ttcatcacct cggtcattct ttgactgacg ctcgacagat
    19981 acagatccat cagatccgaa cccagctcgc ggtacgtctc gatgaagtca atcacacgga
    20041 cggtgtggtc gtagcagtcg cgcagataga gccgggtctc atcgttcaca agcggactgg
    20101 gatcgcgcac cagcgagttc attgcctccc gcagcggcca gatgcaccgc cgcagcgtca
    20161 tcaggttacg tttgatctca tggactcggg cg
//
LOCUS       DAOTPW010000091        20104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2736_length_20104_cov_5.616440, whole genome shotgun sequence.
ACCESSION   DAOTPW010000091 DAOTPW010000000
VERSION     DAOTPW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20104
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_20104_cov_5.616440"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="P5316_09985"
     CDS             <1..953
                     /locus_tag="P5316_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRR85329.1"
                     /translation="GKKIHGTGAIPGGINKNLAIEERDFLLKDIDQMVAWARGALKIA
                     RDYTLENLQTVAPFGSFDSNHLSLIRNDGAMDIYHGNLRAIDAQGNRIFDQVDYNNYL
                     DYIAEEVRPWSYMKFPFIKTLGPENGWYRVGPLARINTADFIDTPEADAALKEFRSAT
                     GNKTCNLTMAYHWARMIEVLHAIEKIKALLHDPDLQGTDLIVKGERRDEGIGLIEAPR
                     GTLFHHYRVNKDDQVVMANLIVSTTNNNEPMNRAVQKVAQDHISGKPEITEGLLNHVE
                     VAIRAYDPCLSCATHALGKMPLTVELVDCHGMLLDRKSRR"
     gene            957..1499
                     /locus_tag="P5316_09990"
     CDS             957..1499
                     /locus_tag="P5316_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRR85330.1"
                     /translation="MGAAAKVLLIGYGNPGRLDDGLGPALAAAVEKLGIPGVTVQEDY
                     QLNVEDAALVAEHDVVVFADADVAGPEPCWFRRIVPRPAWSISTHSIEPRALLALAQQ
                     MFGAKTEGYILGIRGYEFHEFGERLSDRARDNLAAALAFIEPLLRDGSFREAAAGIEE
                     DSVTSTGIISEVDPCKTENM"
     gene            1478..1852
                     /locus_tag="P5316_09995"
     CDS             1478..1852
                     /locus_tag="P5316_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR85331.1"
                     /translation="MQDGKHVILYVDDDQDFLDSMRVLLEANGYKMVEAGSAEEGLKV
                     YRETNPDLIIADLMMEEVDAGTSFVKELRAMGNKAPIYMLSSVGDSLNMATDYQQIGV
                     DGIFQKPINHDALLSVLRAKLK"
     gene            complement(1953..2408)
                     /locus_tag="P5316_10000"
     CDS             complement(1953..2408)
                     /locus_tag="P5316_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85332.1"
                     /translation="MKGMIRATAAVLLLGSAAAWASGLEAGYRSSCRTEADIEDLDAE
                     IRWDGEKWRLLVEYDVEIEHAGAGESFDLVLSLFDKHSRKEAAQIVVPLVQPTEVDDD
                     EVEYESTVEARIDARLVGDPKRLRLRAMVVYRGGGTVLDEKETSVDYKR"
     gene            complement(2626..3987)
                     /locus_tag="P5316_10005"
     CDS             complement(2626..3987)
                     /locus_tag="P5316_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR85333.1"
                     /translation="MSCPAVRVGFLERLGLHRPELRAWAMYDWANSAFVTTIMTAVFP
                     IYYSSVAGAHLTPEASQMRFTLSTTIGLSLIAVMAPLLGAMTDFLPVKKQLIKAFLGI
                     GLVSTAAMYFVGYNDLLLASLLFVVANIAVNGSFVFYDALLPHIAGKDEMDRVSSAGY
                     ALGYVGGGVLLAIQLVWIDPSIVGLPARQPGTPEEGTWPTRVAFLSVAVWWLLFSIPL
                     FRRVPEPPVRLHADGRGTGDLVKASLLQLWATLRDLRGFKDAFVMLLAFLIYNDGIGT
                     IYRLATIYGEGLHFDRTIMIAAIMITQFVGVPFAFLFGSLAGRIGSRPCIFLTLVVYT
                     FISIGGYFMTTSVHFLLLALGVGMVQGGAQALSRSLFASMIPRHKSGQFFGLFAVMEK
                     FAGIMGPAVWTVMLAAGVSGRTPILSVIGFFAVGGILLSLVNVERGRQAARAAEEQAV
                     GGG"
     gene            complement(4243..6729)
                     /locus_tag="P5316_10010"
     CDS             complement(4243..6729)
                     /locus_tag="P5316_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRR85334.1"
                     /translation="MSADMPIMREPELLAPAGDDECLRAAVANGADAVYFGLEDFNAR
                     RRAANFTLARLPEVMGYLHDRNVRGYVAFNTLIFSEELERAAGFVAGIAEAGADAVIV
                     QDLGLLKLIRSMAPTLPIHASTQMTQTHAAGIELLGGMGVRRVILARELSIDEIAKIA
                     RSTTVELEVFVHGAMCISYSGQCLASGSLWGRSANRGVCAQACRLPYRLVVDGQAVES
                     GGRAYLLSAKDLAAWDLIPQLLAVGVSGFKIEGRLKGPHYVAAATSAYRQAIDAAKAG
                     KRFSLGPEREAMLSQSFSRGFTLGFLDGDRHQDLVDGRFPNNRGVDVGRVVGRTRRGV
                     LVELRPEMNDVPLKPGDGVLFDAGLPGADQQGGRIYAVEKADRSDSPVGRRATRVELR
                     FGQGDVNLAAVAIGSVVWKTDDPAVRRELEKTYVRVDPVRRAPLTVRAWISPQRHLQI
                     ELEDDHGRVARVESEAPLAEARRHPMSATLLRAHFSRMGDTPFEPASVQLLHEGVAVE
                     SLPFMAPKSVLNRLRREGVRLLLERRAAESRHEIGDPNVLRAIREQIRDRFRDETGDS
                     GVARPVLCILIRELGQLGAVLEWRAEAGAGRLSVVYADLVDNAECARAVERCRAAGVP
                     VGLATPRICKPGEESLVTGLADLGPDIVLVRNLAALQLLRQYAPGVPLVADHSLNAAN
                     EMTAAALREIGVVRVTACYDLDAAQMIPFAAAWSVLNLEIVVHRHVPMFHMAHCLAAR
                     LSKANDCRQCGRPCRLHRLELRDRLGVDHPVLVDRAGRTTVYNGRVQSAGDLSGKLRA
                     LPVRHWRLELLLEDRGAAHRLIETFAGRME"
     gene            7101..8543
                     /locus_tag="P5316_10015"
     CDS             7101..8543
                     /locus_tag="P5316_10015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRR85335.1"
                     /translation="MTSSRSGSMLIYLALATPLVLVHLGGTDLIRMEPIIALGAKRMV
                     ENGGWFVPHLYDEVYAFKPGLAYWLTAIAGSTWGWSELSLRLPTAICGLLLGLAICLF
                     MGRFLSPRCGLVSGLAAATSCLFLEQARTIGFEMPMALGVGVAILAAIRNLAKDESSL
                     GWWILAGVGLLFGFLAKGLPAIAIYAAGLLTASVALGRIRLLLRWQHLASVAMFILAA
                     AIYGLLAYREGGALIFYQHLVEILFRGTRWKPAMLLQTLLKPAVMFAAYLPGSALLLL
                     RLAPQSPEKPDPTTTRLRIAAWCFLLAGAALFVLSPVDNTRYYLPLMTPLAVLAGIHA
                     ESHRIPMAAFLKEKRRLKATANPVLWMVVAGCIYWLIYVGVVEPRRAYSNSLRAIAAR
                     FAEHVAPNETVYVDSQDEYSSLFWYLDRPVRGSWDIGEPLPPPPAYLLLADKQARLAA
                     SLQNRGLITVAEEKDHEGIPYILCRVQGTP"
     gene            complement(8589..9704)
                     /locus_tag="P5316_10020"
     CDS             complement(8589..9704)
                     /locus_tag="P5316_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR85336.1"
                     /translation="MTGKERMLCALNRGKPDRLPVSVHQWQPYHLDTYLGGISALEAF
                     RRFGMDAQIQYFQLMGQFWLVDADFARFSEQQWVDEVKVISDDPDNRIAHHTITTPEG
                     TMTYKTAGDRKTTWITEYLIKHDEDVDLIRKYMPVPALDPKPISEAYDEVGDDGILRG
                     FVWGDQAGCWQHACCLMDVSELIYRCVDQPDWVHTLLRVLLDKKMRFIESMKGAKFDL
                     VETGGGAGSSTLISPKLHEEFCLPYDRKMHDALHDLGFKITYHTCGGTLGIEEMIVAN
                     GCDASETLAPPSVGGNQEPWEFRRKVGNRLALIGGVDQFNTLTTGSRETIREAVHKLF
                     ETVGRDGGYICSLSDHFFDTPPEKLQVFADAARQCVY"
     gene            9987..11363
                     /locus_tag="P5316_10025"
     CDS             9987..11363
                     /locus_tag="P5316_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR85337.1"
                     /translation="MNRRIWGCAVLLATVGGNQAHAVDFTFAVWSDTHFGANDGGGYR
                     DNSAEDIASLAGKPYPSQIGGTVGQIRFVLVTGDITENTAFAQYQDNDGMTDDDFISC
                     INRWFTMPIYEITGNHDSYNEPGATQIRAAITGRHGNTSYSFDHQGVHFIGLDGWTSG
                     DTPFHSSALPFLEQHMTGVPAQQPVVIFCHYTPRDPPHAQWDRFFNAVQGHNIILMCH
                     GHEHYPRVGRWRGYDYLVTSDCKVVHGNQAFSVVRIRDTQLTGIGYDWYNNTWRTDAL
                     ITKPITGIGPYIIVEPAGFAPATVWSVNPPTDHFTVRNGGGGTLNYQIVTQTAWITVE
                     PTSGSSTGEPDDVSVTYHVEGLVPGDYQATIEVRDPLAFNSPVVLDVRLRVKPVPGDL
                     DVDLDVDQEDFGRFQACLSGPGVAQNAPQCAGARLDGDGDVDADDVAIFRRCLSGPEI
                     PADPGCAE"
     gene            11486..12484
                     /locus_tag="P5316_10030"
     CDS             11486..12484
                     /locus_tag="P5316_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRR85338.1"
                     /translation="MRTKIGADNLADPITAHIHADMVTLRENQTAADAVRAIRRMNIS
                     DKIVYLYVLDKNDRLVGVVPVRRLLGSDPGATIESIMVSPVMSAQTSSSVLEACEMLL
                     NHRFLALPVVDADNRLQGVIDLSQFTDEVLTSAQQQMDSAFQLIGVHVALGRRVSSWR
                     SFRDRFPWLLCNMASGVICAFIAGRFELLLSQVVLLAMFLTVVLALGESVSMQSMTIT
                     LQSLLGRQTGWGQILASARKEFTTSAMLGLGCGGIIGLTAWIWRGQPMPAIAIGGGIC
                     LSIITACFLGVAVPTAIHKMRIDPKVAAGPIVLASADIATLLFYFNLGYWLLSRRV"
     gene            12745..14055
                     /gene="hisD"
                     /locus_tag="P5316_10035"
     CDS             12745..14055
                     /gene="hisD"
                     /locus_tag="P5316_10035"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRR85339.1"
                     /translation="MPRIRVIRTTDPTAMADLATMRQSLLLDHLILDEPPQTKTVRDI
                     IAEVRRRGDDAVSEITARVDHATVPPDQVRVPPERIARAQATTSPALRAAIQKAIEAV
                     RQFQQSILLREPSPIQTDGLTLSLRVRPLRRVGVCVPGAAAPLLSSVIHSALPAQVAG
                     VKEIAVVAPPRHNGDIHPTILAVCGQLGITEVYRMGGAQAIAALALGTRQVPRVDKIV
                     GPGGMYVQLAKRYLYGLVDIDMFAATTEVLIIADRTARPAFVAADMLAQAEHNPGSAI
                     LLTDDESLLDKVLTELDRQLAALPTANAAAKCLEELGAIVIVRNMDEAAEMADQMAPE
                     HLQIETANPRDLADRINAAGAVFLGHFSPESAGDYVAGPSHTLPTGGTARFWSGLSSL
                     SFLRTNSMIEYTAEGIRRDAPAIDLIGRAENLEAHARSATIRAE"
     gene            14152..15198
                     /gene="hisC"
                     /locus_tag="P5316_10040"
     CDS             14152..15198
                     /gene="hisC"
                     /locus_tag="P5316_10040"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRR85340.1"
                     /translation="MSYFRPEIEAMEGYTPGEQPDSLSVVKLNTNENPYPPSPKVMEA
                     IANIKPEQLRRYPHPLGKPFREMAARIFDVTPDHILCGNGMDDILNLTCRAFSGPEAA
                     LVYPVPTYTLYGVLGQIQASIIREVPWGVDYSLPFEALVEARGAVTYLANPNAPTGTF
                     VPVDEVARLADALRGVLCVDEAYVDFADANCMELAKTRPNVLVMRTLSKGYSLAGLRF
                     GFAVGHPELIAGLVKVKDSYNVDAVAIAAASAALADQDYYRLTRRRVINERQRLAAEL
                     EKLGISCLPSKSNFLFARPTRPSAARLYESLKARGILVRYFNSPGIDDRLRITVGTPE
                     QNDILIAALKELVS"
     gene            15339..15911
                     /gene="hisB"
                     /locus_tag="P5316_10045"
     CDS             15339..15911
                     /gene="hisB"
                     /locus_tag="P5316_10045"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRR85341.1"
                     /translation="MHRSTKETRIEVAIDLDGTGRVVAETGIGFFDHMLNHIGRHGLF
                     DLSVKASGDLHVDYHHTVEDVGICLGQALAQAVGDKKGIRRYGFFIAPMEESQAIVAV
                     DLSGRTAVVYNAKYRGSKIGDFDVELVEEFLRALAANAKMNLQVTVPFGTNNHHVAEA
                     IFKALGKSLRQAVEIDPREESVPSTKGILV"
     gene            complement(15967..17202)
                     /locus_tag="P5316_10050"
     CDS             complement(15967..17202)
                     /locus_tag="P5316_10050"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85342.1"
                     /translation="MKVPLHQRWWRTALIAALLAGGLYALLGPRGAKSGQHALTVFVG
                     AYLTVWFCNPAGFFRRMSWVALLMVGGVVFIYLLHTKGYFGPHASAAFVISNAVWLIG
                     ILSGAALFFAVIEYLQHRSPSDLDAVGDEIPEPESFSRGRRKDDELPRFRPPTPFKRR
                     ERVPDQETLAKIAERYASMTATSEEPRLTVRMLRSERDRLIDRLDAALARADGLARSG
                     DAEAKAAAEAARSRLDVEPLQRFLAAEAERRGCKTREDATAYVAFCREIAGVAEMRGD
                     WETARQHLEQVIRLVPEDVDAYCRLGRACLMLNKLVDAEEAYQKILMLSSEDEWRAMA
                     FTNMGTVQLLSGSMEEAERVFNRALEIDERMGDVEGMAANYANLGHVCRRRGNLGKAE
                     KMFSAALEIDEGLELSLVR"
     gene            complement(17296..18225)
                     /locus_tag="P5316_10055"
     CDS             complement(17296..18225)
                     /locus_tag="P5316_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRR85343.1"
                     /translation="MNSTSIGVLAEDPLARKAGSGAVQGRSPGWMVLRTILLIGLASS
                     MTVPFVWMLLASFKPPHEADRPEFLPEQWKWENYPIVLGLTPDSRGEKLPIRFGRWYF
                     NSLFVASWVTFLQVVTSAMAAYAFARLKWRGRDVVFLAYLATVMVPAVVLLIPNYYIM
                     FRLGLLDSYLGLILPAAFSAFGTFLLRQFMLNIPTSLDEAAEIDGASRWQVFVEVILP
                     LSRPGLIVLAIMTFLGNYASFFWPLVMVKSDHLRTLPVGMMWFENLGGAGYGRQTELM
                     MAATVMNIVPLMVLFVVLQRYLVRGIQLGAVKG"
     gene            complement(18233..19363)
                     /locus_tag="P5316_10060"
     CDS             complement(18233..19363)
                     /locus_tag="P5316_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRR85344.1"
                     /translation="MGLRLSRRSRRDFGWAMLFLLPNIMGFAVFVAAPVVFSLVMAFT
                     NWDLAQREPFGFVGFENFERLFFGPEAKDFRKAFGTTLYFMLGMPVSIVGSLVLAVLL
                     SDPIYLGSRRVRRLLIGASLAATCAGLMVCAGTGRATTGLALAIIGASAAVATVFNRV
                     GLRTLFYVPFFTAGVAQYILWKLMFRPDSGPINASLRVLFAVLGLDIEPPNWLGSTAN
                     LWCLDPQTGLPAAGHFGLGARDAIVIMGIWATVGGNNMLLYLAGLANVPQDLYEAAMI
                     DGAERWKSFRHVSWPQLAPTTFFIVIMSIIGGLQGGFEQAKIMTEGNPAETTISLGFY
                     IYLTGFADFRLGLASAIAWTMFVMILAVTLVQWRFGSRQINI"
     gene            complement(19385..>20104)
                     /locus_tag="P5316_10065"
     CDS             complement(19385..>20104)
                     /locus_tag="P5316_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85345.1"
                     /translation="RNAIGTQGGWGSSVIDLFCTERIAMLRMGRWGMIKFRTQPNLRG
                     NIGAVLLPHMRSGADEIRALSVGINPKSPNKNEALRFLQFLASDEFSWQVVESADALP
                     PSPKVGQDERFLKDAAHPEEDFNHLFIEAIRRGRSNHFPPFIQTTRVERIFNRHLAVM
                     SDRKTTPEQMCRALTDEINEEMRVSLTRYASMREEYKRRTGRDFDPADYPPKGDRPSA
                     GRNPSAPGSRNAHARRTDERP"
BASE COUNT         4111 a         6991 c         5492 g         3510 t
ORIGIN      
        1 ccggcaagaa gatccacggc accggcgcca tccccggcgg catcaacaag aacctcgcga
       61 tcgaagagcg cgatttcctg ctcaaggaca tcgatcagat ggtcgcctgg gcacgcggag
      121 ccctgaagat cgccagggac tacactcttg agaatctcca gaccgtcgcc ccgttcggct
      181 cattcgactc gaaccacctg tccctgatcc gcaacgacgg ggccatggac atctaccacg
      241 gcaacctgcg ggccatcgac gcccagggca acaggatctt tgatcaggtc gactacaaca
      301 actacctcga ctacatcgcc gaggaagtcc gaccctggtc ctacatgaag ttcccgttca
      361 tcaagaccct cggccccgaa aacggctggt accgcgtcgg ccccctggcc cgcatcaaca
      421 ccgccgattt catcgatacc cccgaggccg atgccgcact caaggagttc cggtccgcca
      481 ccggcaacaa gacctgcaac ctgacgatgg cgtaccactg ggcacgaatg attgaagtgc
      541 ttcacgcgat cgaaaagatc aaggccctgc tgcacgaccc cgatcttcag ggcaccgact
      601 tgatcgtcaa gggcgagcgc cgcgacgagg gcatcgggct gatcgaggcc ccgcgaggaa
      661 cgctcttcca ccattaccgt gtcaacaagg atgaccaggt ggtgatggct aacctcatcg
      721 tgtcgaccac aaacaacaac gagccgatga accgcgccgt ccagaaggtc gcccaagacc
      781 atatctccgg caagccggag atcaccgagg gtctgctcaa ccacgttgag gtggccatcc
      841 gggcatacga tccgtgtctg tcgtgcgcga cacatgccct cggaaagatg ccgttgaccg
      901 tcgagctggt cgactgccac ggcatgttgc tcgatcgcaa gagccggagg taaaacatgg
      961 gcgccgcggc caaggttttg ctgatcgggt acggcaaccc cggccgcttg gacgacgggc
     1021 tcggtcccgc gctggccgcg gccgtcgaga agctcggaat ccccggcgtg accgtgcagg
     1081 aggactacca gctcaacgtc gaagatgcgg ccttggtcgc cgagcacgac gtggtggtct
     1141 tcgcggacgc cgacgtggcc ggaccggaac cctgctggtt ccggcggatc gtgcccaggc
     1201 ccgcgtggag catctccacg cacagcattg agccgcgggc cctcctggcc ttggctcaac
     1261 aaatgttcgg ggcgaaaacc gagggttaca tcctcggaat tcgcggatat gagtttcatg
     1321 aatttggaga aaggctgtcc gaccgggccc gcgataatct tgccgccgca ctggcattca
     1381 tcgagccgct gctccgcgac ggatcgttcc gcgaggcggc cgcgggaatc gaggaagact
     1441 cggtcacgag caccggaata atcagcgagg ttgacccatg caagacggaa aacatgtgat
     1501 tctctacgtc gatgacgacc aggacttcct ggactcgatg cgcgtactcc tcgaggccaa
     1561 cggatataag atggtcgagg ccggcagcgc cgaggaaggc ttgaaggtct acagggagac
     1621 aaaccccgac ctcatcatcg ccgacctgat gatggaagag gtcgacgccg gaaccagctt
     1681 cgtcaaggag cttcgggcca tgggcaacaa ggcccccatt tatatgctca gctccgtcgg
     1741 cgacagcctc aacatggcca ccgactacca gcagatcggc gtcgatggca tcttccagaa
     1801 acccatcaat cacgacgcgc tgctgtcggt cctgagggcc aagctcaaat gagaacctat
     1861 gcaagaaccc gtatggaatc ggcttccagc cggtcgcctt tcggggtttt gggtaggttt
     1921 caagccgtca gccggtccgc ctggagacgg gctcaacgct tgtagtccac cgaggtctct
     1981 ttctcgtcga ggacagtccc cccgccacga tacacgacca tggctcgcag gcgcagccgt
     2041 ttagggtctc cgaccagcct cgcgtcgatg cgagcctcca cggtgctctc gtactcgacc
     2101 tcgtcgtcat cgacttcggt gggttgcacc agcggaacaa caatctgtgc cgcctccttg
     2161 cggctgtgtt tgtcgaacag actcaacacc agatcgaacg attcgcccgc gcccgcatgc
     2221 tcgatctcaa cgtcatactc gaccagcaat cgccacttct cgccgtccca ccggatctcc
     2281 gcgtccagat cctcgatatc ggcctctgtc ctgcaactgg accgatagcc ggcttcgagc
     2341 ccgcttgccc aggcggccgc cgaacccagc agaagaaccg ccgctgtcgc tcgtatcatt
     2401 cctttcatcg ctgattcgct ccttgacgcc atggcgctga cgcccggttt ccctcaccca
     2461 gacagacacg ccccgatgcc ggaagttgga cccgaggcgg cttcgttccc tcccctacac
     2521 gcggtggaaa tcgccctatc tcacgtagcg gaaaggcttt atcgccccgc cgacgggatc
     2581 tgttcgtgac cgcaacatct ccctgccgac ggccgggaac cagactcagc cgccccccac
     2641 cgcctgctcc tccgccgccc gggccgcctg ccgcccgcgt tcgacgttca caagtgatag
     2701 caggataccg cccaccgcaa agaaaccgat gaccgacagg atgggcgtgc gaccggaaac
     2761 gccggctgcc agcatgaccg tccaaaccgc cggccccatg atccccgcga acttctccat
     2821 caccgcgaac aggccgaaaa actgccccga cttgtgccgc ggaatcatgc tcgcaaacaa
     2881 ggaccgactc agcgcctgcg cccccccctg caccatccca acacccagcg ccagcagcag
     2941 gaaatgcacc gacgtggtca tgaaataccc gccgatgctg atgaacgtgt agacaacgag
     3001 tgtcaaaaag atgcacggcc ggctgccgat tcgcccggcc agactgccga acaggaatgc
     3061 gaacggcacg cccacaaact gcgtgatcat gatcgccgcg atcatgatcg tgcgatcgaa
     3121 atgaagtccc tccccgtaaa tcgtggccag ccggtaaatg gtcccgatgc cgtcgttgta
     3181 gatcagaaag gccagcagca tcacaaaagc atctttgaac cctcgcagat cccgcagcgt
     3241 ggcccaaagc tgcaacagcg atgccttgac taaatcaccc gttccgcgac cgtccgcatg
     3301 caagcgaacc ggcggctcgg gcacgcgccg aaacagcggg atcgaaaaaa gaagccacca
     3361 caccgccacc gaaaggaacg ccacccgcgt gggccacgtc ccctcctcag gtgtcccagg
     3421 ctgccgcgca ggaaggccca cgatactcgg atcaatccac accagttgga tcgccagcaa
     3481 cacccccccg ccgacatagc cgagggcata gccggcgctc gacactcggt ccatttcgtc
     3541 cttcccggca atatgcggca gcaaagcgtc gtaaaagacg aaactcccgt tcaccgcgat
     3601 gttggccaca acaaacagca acgatgccaa gagaagatcg ttgtagccga caaagtacat
     3661 cgcagccgtc gaaaccagcc cgatgcccag aaaagccttg atcagttgct tcttgaccgg
     3721 caggaaatcg gtcattgccc cgagcagcgg cgccatcacc gcaatgagac tcaagccgat
     3781 cgtcgtcgaa agcgtgaacc gcatctgcga ggcttccggc gtcagatgcg ccccggccac
     3841 gctcgaatag tagatcggaa acacggcggt catgatcgtg gtcacaaacg ccgagttcgc
     3901 ccagtcgtac atcgcccagg cccgcagttc cggccggtgc agaccgaggc gctccagaaa
     3961 cccgacgcga acggccgggc atgacacaac tcgtcttccg ctcgatgact cagaatgaca
     4021 ggaccggtcg gcactcatcg ccgcggacta taacaccgcc gaaatcgcct gtcacgatcc
     4081 cgcgtcgaac tcgcgcacgc agctcgtgag aggacaagtc acggaacaga cggaaatgcg
     4141 ggtagtgatc cgtgatcacc acaggagaag actgaaaacc gagcacggaa cacggacgaa
     4201 ttgacttcgc tatcgggatt ctggccgtct gagcttctcc cgtcattcca tccggccggc
     4261 aaaggtctca ataagcctat gggcagcccc tcggtcttcg agaagcagct ccagccgcca
     4321 gtgcctgacg ggcaacgccc gaagcttccc cgaaagatct cctgccgact gaacgcgacc
     4381 gttgtaaacc gtcgtacgac ccgcccgatc gaccagtacc ggatgatcga cgccgagccg
     4441 atcccgcaac tcgagtcggt gcagccggca cggacgaccg cattgccggc agtcgtttgc
     4501 ctttgaaagt cgcgccgcaa gacaatgagc catgtggaac atcggaacgt gccgatgaac
     4561 gacgatctcc agattcagca ccgaccacgc cgcggcaaac ggaatcatct gagcagcgtc
     4621 cagatcatag caagccgtca cccgaacaac gccaatctcc cgtaatgctg cggccgtcat
     4681 ctcgttggcc gcattcagcg agtggtcggc aaccaacggc acaccaggag catactgtcg
     4741 cagcagctga agcgccgcca gattccgcac cagaactatg tccgggccga ggtccgccaa
     4801 accggtcacc agcgattctt ctcctggttt gcagatccgc ggcgtcgcta aacccacggg
     4861 cacccccgcc gcacggcatc gttcgactgc tcttgcacac tcggcgttgt caacgaggtc
     4921 cgcgtacacg accgacaacc gacccgcccc ggcctcggca cgccactcca gaacggcacc
     4981 caattgcccc aattcccgaa tcagaatgca tagaaccggc ctcgccaccc cggaatcgcc
     5041 ggtttcgtcg cggaaccgat cacgtatctg ctcgcgaatc gccctcagca cgttcgggtc
     5101 gccaatctcg tggcgagact cggcggcccg ccgctcaagc aacagcctca ccccctcacg
     5161 gcgcagccgg ttcaacacgc tcttgggagc catgaacggc agactctcga ccgcaacacc
     5221 ttcgtgcaac agttggaccg acgccggctc gaatggcgta tcccccatcc ggctgaaatg
     5281 cgccctcaga agcgtagcgc tcatcggatg acgtctggcc tccgccagag gtgcctccga
     5341 ttccactcgg gccacgcggc catggtcatc ttccagctcg atctgcaaat gacgctgcgg
     5401 cgaaatccag gcccgaaccg tcaaaggcgc acggcggacg ggatcgaccc tgacgtacgt
     5461 tttctccagc tcacgtcgga cggccgggtc gtccgttttc cacaccacgc tcccgatcgc
     5521 cacagccgcg aggttcacgt ccccctgacc gaaccgcagt tccactcggg tcgcccgacg
     5581 ccctactggt gaatctgacc gatctgcctt ctcaaccgcg tatatccgcc cgccctgctg
     5641 atccgctcca ggcagcccgg catcaaaaag cacgccgtcg cccggcttca gaggcacgtc
     5701 attcatctcg ggccgcaact caaccaacac cccccgcctc gttctgccga ccaccctgcc
     5761 gacatcgact cctcgattat tggggaatcg accgtccacc aggtcttgat gccggtcacc
     5821 gtcgagaaaa ccgagggtga agcctcgcga gaaactctgc gacaacatcg cctctcgctc
     5881 agggccaagg ctgaacctct ttccggcctt cgccgcatca attgcctgac gataggcact
     5941 cgtggccgcc gcgacatagt gcggaccttt caaacggcct tcgatcttga atccgctgac
     6001 ccccacggcc agcaattgcg gaatcaaatc ccacgccgcc agatccttcg cgctgagcag
     6061 gtatgcccgg ccgcccgact cgacggcttg gccgtcaaca accaggcgat acggcaaccg
     6121 gcaggcttgt gcgcaaacgc cgcggttggc gcttcgaccc catagcgacc cgctggccag
     6181 acactgaccg ctgtacgaga tgcacatggc cccgtgaaca aaaacctcca actccacggt
     6241 cgtggagcgc gcgatcttcg cgatctcgtc aatggacaat tcgcgggcaa ggatgacccg
     6301 ccgcacgccc atcccaccca gcaactcgat gcccgcggcg tgtgtctggg tcatttgagt
     6361 ggacgcatga atgggcagtg tcggcgccat cgaccggatg agcttcaaca gacccagatc
     6421 ctgaacgatc accgcgtccg ctccggcttc ggcgataccg gcaacaaagc cggcggcccg
     6481 ctccagctcc tctgagaaga tcaacgtgtt gaaggcgacg tacccccgca cgttgcgatc
     6541 gtgcagataa cccatcacct cgggcaagcg agccagcgtg aagttcgcag cccggcgacg
     6601 ggcattgaag tcctccaacc cgaagtatac ggcgtcggca ccgttggcca ccgcggcacg
     6661 caggcattcg tcatcgcccg ccggcgccag cagttcgggc tctcgcatga ttggcatgtc
     6721 agccgacatc agaccaggct ccaggcgcgt tattgaaccg actcatgata gcagagcaac
     6781 ggctccctcg acgagccggc gctttgtacc cgttgcacaa ccgactcgac cgcggatcgc
     6841 tatcatcgcc caaactgctg caaaccctga tcttcgccgc agcgtccttg cctgttgtgc
     6901 aagaccatcg ttcctggtag aagtacgcaa ggccgcccgt gttccggcgg cccgacgcgg
     6961 ggaaagcact tggaccgcca aaaacgcggc gtcttcacca tccgcaagcg ccgagtcgtg
     7021 gcgattcgtg cccttaggag gtttcggcta cggtacacaa caaaacagaa gagtccgtgc
     7081 agccttccaa agcagtctcg atgacttcat cgcgatccgg gtcgatgctc atctatctgg
     7141 ctctggcgac cccccttgtt ctcgtccacc tgggcggcac cgatctgatc cggatggagc
     7201 cgatcattgc cctgggtgcc aaacggatgg ttgagaacgg cggctggttc gttccgcact
     7261 tgtacgacga ggtctatgcg ttcaaacctg ggctggccta ctggttgacc gcaatcgccg
     7321 gctcgacgtg gggctggtcg gagctctcac tgcgcctgcc gacggcaatc tgcggcctgc
     7381 tgctgggctt ggcgatctgc ctcttcatgg gacgcttcct gtccccccga tgcggcctgg
     7441 tcagcggctt ggcggcagcc acgtcctgcc tcttcttgga gcaggcccgc accatcggtt
     7501 tcgagatgcc catggctctc ggcgtcggcg tggccatcct cgcggcaatt cgcaacctcg
     7561 ccaaggacga atcgagcctg gggtggtgga tactcgccgg cgtcggcctg ctcttcggtt
     7621 tcctggccaa gggcctccct gcaatagcga tctacgccgc cggcctgctg accgcctccg
     7681 ttgctcttgg acggattcga cttctcctgc gttggcaaca ccttgcttcg gtggcgatgt
     7741 tcatcctggc ggcggcaatc tacggtctgc tggcctatcg cgaaggtgga gccctcatct
     7801 tctaccagca cctcgtggag atcctctttc gcggcacgcg atggaagccc gcgatgctgc
     7861 tgcagactct gctcaaaccc gccgtgatgt tcgccgccta cctgcccgga tcggccctgc
     7921 tgctcctgcg attggcccca caatccccgg aaaagcccga ccccacgaca accaggcttc
     7981 gcatcgcagc ctggtgtttc ctgctcgccg gcgcggcctt gttcgtcctc tcgccggttg
     8041 acaacacccg ttactatctc ccgctgatga cgccgctggc cgtcttggcc ggaatccatg
     8101 ccgagtcgca ccggatcccc atggccgcgt tcctcaagga aaaacgccgg cttaaggcga
     8161 cggccaaccc cgtgctctgg atggtcgtcg caggctgcat ctattggctt atctacgtcg
     8221 gcgtggtcga gccgcgccgc gcgtacagca actcgctgcg ggccatcgcc gcacggtttg
     8281 ccgaacacgt agcgccgaac gagaccgtgt acgtggactc acaggacgag tacagctcgt
     8341 tgttctggta cctcgatcgc ccggtccgcg gatcctggga catcggcgag cccctgccgc
     8401 cgccccccgc ataccttctc ctggccgaca agcaggccag gctcgccgcc tcgctgcaga
     8461 acagaggcct catcaccgtc gccgaagaaa aagaccacga agggatcccc tacatcctct
     8521 gccgcgtcca gggtaccccc tgaccacagc ccagtcggcg gaatcccctt tcgaaccagc
     8581 ctgctctctc agtaaacaca ttggcgggcc gcatccgcaa aaacctgcag cttctccggc
     8641 ggcgtgtcaa aaaaatgatc gctgagcgaa cagatgtatc caccgtcgcg ccccaccgtc
     8701 tcgaacagct tgtgaaccgc ctcccgaatg gtctcccggc tgccggtggt aagcgtgttg
     8761 aactgatcca cgcccccgat cagcgccagg cggttgccca cctttctcct gaactcccac
     8821 ggctcctggt tgccgccgac gctgggcggc gcgagggtct ccgaagcgtc gcagccgttg
     8881 gccacgatca tctcctcgat gcccagcgtg cccccgcacg tgtgataggt gatcttgaac
     8941 cccaggtcat gcaaagcgtc gtgcatcttg cgatcgtacg gaagacagaa ctcctcgtgc
     9001 agcttcggcg aaatcagcgt cgacgagccc gccccgccgc ccgtctcaac aaggtcgaac
     9061 ttcgccccct tcatcgactc gataaaccgc atcttcttgt ccagcagaac tcgcagcagc
     9121 gtatgcaccc agtccggctg gtccacgcag cgatagatca gctcgctcac gtccatcagg
     9181 cagcaggcgt gttgccagca ccccgcctgg tcgccccaca cgaagccgcg aagaatcccg
     9241 tcgtcgccga cttcgtcgta cgcctcgctg atcggtttgg gatcaagcgc cggcaccggc
     9301 atgtacttgc ggatcaggtc gacgtcttcg tcgtgcttga tgagatactc ggtgatccag
     9361 gtcgtcttgc ggtccccggc cgtcttatag gtcatcgtgc cttccggcgt cgtgatcgtg
     9421 tgatgggcga tgcggttgtc cgggtcatcg ctgatcacct tgacctcgtc cacccactgc
     9481 tgttccgaga acctggcgaa gtcggcgtcc accagccaga actgccccat cagttggaaa
     9541 tactgaatct gggcgtccat gccgaacctg cgaaacgcct ccagggcgct gatcccgccc
     9601 aggtaggtat ccagatgata gggctgccat tggtgaaccg acaccggcaa ccgatccggc
     9661 ttgccgcgat tcaaagcaca aagcatccgt tccttgccgg tcatgagcat gctccaaacg
     9721 cctgataccg cagatgctaa tgccggctcc tgaaatgtgc cagaatccgg ccaaacgcct
     9781 tcgcatctcg tcctgctgtc ggacagtggg ggtcgcacgt gccaaggccg gctcaataat
     9841 gtgaacgaca agcgtcaggc cgcccaaaaa acgcttgtcc atccggcccg actctcgtaa
     9901 catgaacggt agagtcgaag aggcgcttcc tgcgaggttc gcgccaagag cccgcgtggc
     9961 accgcaaatc cgtgagcccg accgtcatga atcgccggat atggggttgt gccgttctgc
    10021 tggcaaccgt cgggggcaac caggcccacg ccgtcgactt cacgttcgcc gtctggtccg
    10081 acacccactt cggagccaac gacggcggcg gttatcgcga taactcggct gaagacatcg
    10141 cctccctcgc cggcaagccc tacccatctc agatcggcgg cacggtcggc cagatccgct
    10201 ttgtcctggt caccggagac atcaccgaga acaccgcttt cgcgcagtac caggacaatg
    10261 acggaatgac cgacgacgac ttcatctcat gcatcaaccg ctggttcacg atgccgatct
    10321 acgagatcac cggcaaccat gattcctaca acgaaccggg tgccacccag atccgcgccg
    10381 ccatcaccgg ccggcacggc aacacctcct acagcttcga ccatcagggc gtccatttca
    10441 tcggccttga cggctggacc agcggcgata cgcccttcca ctcttcggcc ctgccgtttc
    10501 tcgaacaaca catgaccggc gtccccgctc agcagccggt cgtcatcttc tgccactata
    10561 ccccgcgcga cccgccccac gcccaatggg accggttctt caacgccgtg cagggacaca
    10621 acatcatcct catgtgtcac ggtcatgaac actacccgcg ggtaggccga tggcgaggct
    10681 acgattacct ggtcacatcc gactgcaaag tcgtgcacgg aaaccaggcc ttctccgtgg
    10741 tgcggatcag agatacgcaa ctgaccggca tcggctacga ctggtacaac aacacctggc
    10801 gaaccgacgc cctgatcacc aaaccgatca ccggaatcgg cccctatatc atcgtcgagc
    10861 ccgccggctt tgcaccggcc accgtatgga gcgtcaaccc cccgaccgat cacttcaccg
    10921 tgcggaacgg gggcggcgga accttgaact accaaatcgt cacccaaacg gcctggatca
    10981 ccgtcgagcc gacatccggc agcagcaccg gcgaacctga cgatgtcagc gtcacctacc
    11041 acgtcgaggg gttggtgccg ggagactacc aggcgaccat cgaagtccgc gaccccctcg
    11101 cgttcaactc gcctgtcgtc ctcgacgtcc gactgcgcgt caaaccggtc cctggcgacc
    11161 tcgacgtcga tctggacgtc gaccaggagg acttcggtcg gttccaggcc tgtctcagcg
    11221 gtccgggcgt cgcacagaac gcgccgcagt gcgccggcgc gcgactcgac ggcgacggcg
    11281 atgtcgacgc cgacgatgtc gccatcttcc gccgctgcct cagcggaccg gagattccgg
    11341 ccgatcccgg ctgcgccgaa tgaggtcggc tcggaccttc tccatttgcc tgcaacgcca
    11401 aaacgcccat aatgtcggcc acgcggccgt ttgacctggc ccggtgcacc aggcccgcgc
    11461 gaattcgtcc cggcaggaaa catccatgag aacgaaaatc ggtgcggata atctggctga
    11521 tccgatcacc gctcatattc acgccgatat ggtcacactg cgcgaaaacc agaccgccgc
    11581 cgatgcggta cgcgccatcc gccggatgaa tatctccgat aagatcgtct acctgtacgt
    11641 gctcgacaag aacgaccgcc tggtcggagt agtaccggtg cgccgactgc tgggcagcga
    11701 cccgggcgcg accatcgaat ccatcatggt ctcccccgtc atgtcggctc agacttccag
    11761 ctccgtgctc gaagcctgtg aaatgctgct gaaccaccgc tttctggcgc tgcccgtggt
    11821 cgatgccgac aatcgcctcc agggcgtcat cgatctcagc cagttcaccg acgaagtgct
    11881 gaccagcgcc cagcagcaaa tggacagcgc cttccagttg atcggcgtac acgtcgccct
    11941 gggccgccgc gtctcatcct ggagaagctt tcgcgaccgc tttccctggc tgctctgcaa
    12001 tatggccagc ggcgtcatct gcgccttcat cgccggccgc ttcgagttgc tgctcagtca
    12061 ggtggtgctt ctggctatgt ttctcaccgt tgtcctggcc ctcggcgaga gcgtcagcat
    12121 gcagtccatg accatcacgc tccagagcct gctggggcgc cagaccggct ggggacagat
    12181 ccttgcctcg gcccgaaagg aattcacgac ctccgccatg ctgggactcg gctgcggcgg
    12241 catcatcggc ctcaccgctt ggatctggcg gggtcagccc atgccggcca tcgccatcgg
    12301 cggcggcatc tgcctttcca ttatcaccgc ttgcttcctg ggcgtggccg ttccgaccgc
    12361 cattcacaag atgaggatcg accccaaagt ggccgccgga cccatcgtcc tggcatccgc
    12421 cgacatcgca acccttctct tctacttcaa cctgggctac tggctgctga gccgccgggt
    12481 ttgacccaag aggctgaaag agaacctccg tttccgtcgt cactccaact cccggacgcg
    12541 gggcccaccc tgccaacagc accgacatcc accctcgtgc atgggcgccc tgccatccgt
    12601 tcatcgattc tgccatttgg ctggctcgct cccgtgtgcc tcacttgacg aaactggcca
    12661 gacccagccc atgctcttat aatcgcatac tttggggtaa gcccgggctc gggcaagccc
    12721 ttgttaggca gaacgagcgt caagatgccc agaatccgcg tcatcagaac gaccgatccc
    12781 acggcaatgg ctgacctggc aacgatgcgg cagtcgttgc tgttagacca cttgatcctc
    12841 gatgagccgc cccagacaaa aaccgtccgc gatatcatcg ccgaagttcg ccgtcgcggc
    12901 gacgatgccg taagcgagat caccgcccgc gtcgaccatg ctaccgtccc gcccgatcag
    12961 gtacgcgttc cgcccgaacg gatcgcccgt gctcaggcga cgacgtcacc cgccctgcgg
    13021 gcggcaatcc agaaagcgat cgaggccgtt cggcaattcc aacagtcaat cctgttgcgc
    13081 gagccgtcgc cgattcagac cgacgggctt acgctttccc tgcgggtacg accgctgcgc
    13141 cgcgtgggcg tatgtgttcc gggcgccgca gcccccctgc tgtcttccgt catccacagt
    13201 gccctgcccg cacaagtcgc cggcgtcaag gaaatagccg tcgtcgcgcc tccccgtcac
    13261 aacggcgaca tccaccccac gatcctggcc gtctgcggcc aactcggcat caccgaggtc
    13321 taccgcatgg gcggggccca ggccatcgcc gcgctggcac tcggaacccg gcaggttccg
    13381 cgagtcgaca agatcgtcgg ccccggaggc atgtacgtgc aactggcaaa gcgatatctc
    13441 tacggcctgg tcgacatcga tatgttcgct gcgaccaccg aagtattaat catcgccgac
    13501 cgtaccgcgc gaccggcctt tgtcgcagcc gacatgcttg cccaggccga gcacaacccg
    13561 ggctcggcga ttctgctgac cgacgacgaa agcctgttgg ataaggtcct gacggaactc
    13621 gatcgccaac tggccgccct ccccaccgca aacgcggccg cgaagtgcct tgaagaactc
    13681 ggcgccatcg tcatcgtccg caacatggat gaagcggctg agatggccga tcagatggca
    13741 ccggagcacc tgcagatcga gacggccaac ccgcgagacc tcgccgatcg tatcaacgcc
    13801 gccggagccg tcttcctggg ccacttctcg ccggaatcag ccggcgacta cgtggccgga
    13861 ccgtcccata ccctgcccac gggcggcacg gcaaggtttt ggagcgggct ctcctcgctg
    13921 agcttcctgc gaaccaacag catgatcgaa tatactgccg agggaatacg acgggatgcg
    13981 ccggcaatcg atctgatcgg ccgtgccgag aatctggagg cccacgcccg ttcggccacg
    14041 attcgtgccg agtagaataa ccgggctctc catgaaagtt gatcgcgacc tgacagagct
    14101 taccggaatc cgatcgctta ctgctgaagg cggatagctg agtgctgatg tatgagttac
    14161 tttcgacctg aaatagaagc gatggaaggc tacacccccg gcgagcagcc ggacagcctc
    14221 tccgtcgtca agctcaacac caacgaaaat ccctaccctc cctcgccaaa ggtgatggag
    14281 gctatcgcga acatcaagcc ggagcagttg cggcgatatc cgcatcctct gggcaagccc
    14341 tttcgcgaaa tggcggcaag aatcttcgac gtaacccccg accacatcct ctgcggcaac
    14401 gggatggatg acattctcaa tctgacctgc cgggccttca gcggaccgga ggcggcgctc
    14461 gtctaccccg tgcccacgta taccctgtac ggcgtcctcg gacagatcca ggcttccatc
    14521 atccgcgagg ttccctgggg ggtagactac tcccttccgt tcgaggccct tgtcgaggcc
    14581 cgcggagccg tcacctacct ggccaatccc aacgccccca cgggcacttt cgttcccgtg
    14641 gacgaggtag cccgactggc cgatgctttg cgcggcgttc tctgcgtcga cgaggcatac
    14701 gtcgatttcg ccgatgccaa ctgcatggaa ctggccaaaa cacgcccaaa cgtgctggtc
    14761 atgcgaacgt tatccaaggg ttactcgctg gccggcctgc gattcggttt tgccgtcggc
    14821 cacccggaac ttatcgccgg cctggtcaag gtcaaggaca gctacaacgt ggacgctgtc
    14881 gccatcgccg cggcatcggc cgctctggcc gaccaggatt actaccgcct gacacggcga
    14941 cgcgtgatca acgaacgaca acgcctggcg gcggaactcg agaaactcgg catctcatgc
    15001 ttgcccagca agtcgaactt cctcttcgcc cgccccacca ggccgagcgc ggctcggctg
    15061 tacgaatcgc tcaaggctcg cggaatcctc gtccgatact tcaacagccc gggcatcgat
    15121 gaccgtttgc gcatcaccgt cggcacgccc gaacaaaatg atatcctcat cgccgctctc
    15181 aaggagttgg tctcgtgagc aggaaagcgt ctaagccggc aggacaaggc aaaagggtgc
    15241 cgcagcgaaa aaggggacag gacccttttg tgcatagcac ccgaaagacc gcctcgcggc
    15301 aaaaggatcc tgtccccttt tgcgggcgaa aagcagtggt gcatcgctca accaaggaaa
    15361 cccggatcga agtcgccatc gatctcgacg gcaccggccg cgttgtcgcg gagaccggca
    15421 tcggcttctt cgatcacatg ctcaaccaca tcggccggca cggcttgttc gatctgtcgg
    15481 tcaaggccag cggcgacctg cacgttgatt accaccacac cgtcgaggac gtgggcatct
    15541 gcctcgggca ggcgctcgcc caggcggtcg gcgacaagaa aggcatccgc cgttacggct
    15601 ttttcatcgc cccgatggag gagtcgcagg ccatcgtggc cgtcgacctc tccggccgca
    15661 cggccgtggt gtacaatgcc aagtaccggg gctccaaaat cggcgacttc gacgtcgaac
    15721 tggtcgagga gtttctgcgt gcccttgcgg ccaacgccaa gatgaacctg caggtcaccg
    15781 tccccttcgg caccaacaac caccacgtgg ccgaggccat cttcaaggcc ctgggcaaat
    15841 cccttcgcca ggcagtcgag atcgatccgc gagaggaatc cgttccgtca accaaaggca
    15901 tcctggtgta gaaaccgcga aatcacaccg tcgcgcggcg gcgcgcaaac ccctccctga
    15961 gccccctcag cggaccaatg acagttccag cccttcgtcg atctccagcg ccgcgctgaa
    16021 catcttctca gccttcccca gattgccccg ccttcggcag acatggccca gattggcata
    16081 attcgcagcc atgccctcca cgtcccccat ccgctcatca atttccagcg cccggttgaa
    16141 aacccgctcg gcctcctcca tcgagccgga caaaagctgc accgtgccca tgttcgtaaa
    16201 cgccatcgcc cgccattcgt cctcagaaga caacatcagg atcttctggt aagcctcctc
    16261 ggcatcaacc agcttgttga gcatcaggca ggcacgcccc agccgacaat atgcgtccac
    16321 gtcctccggc accaaacgga taacctgctc aagatgctgc cgggcggttt cccagtcgcc
    16381 ccgcatttcc gccacacccg caatttcgcg gcagaaagcc acgtaggcgg ttgcgtcctc
    16441 gcgagtcttg cagcctcgac gctcggcctc ggcagccaga aaccgctgca gcggctcaac
    16501 atccagtcga cttcgcgcgg cctctgccgc cgccttcgcc tcggcatcgc cgctccgagc
    16561 gagtccgtcc gcacgagcca gggccgcatc cagccgatcg atcagccgat cccgctcaga
    16621 ccgcagcatt cgcacggtca accgcggctc ctcggaggtt gccgtcatcg aggcataacg
    16681 ctctgcaatc ttcgccaacg tttcctggtc cggaacccgc tccctgcgtt tgaacggagt
    16741 cggcggccga aaccgcggca actcatcatc cttccgccgg ccccttgaga aagactcggg
    16801 ctcgggtatc tcatctccca ccgcatccag atctgacggg ctgcggtgtt gaagatactc
    16861 gatgacggca aagaacagcg ccgccccgct caagatccct atcagccaga ccgcgttcga
    16921 gatgacgaag gccgccgacg cgtgcggccc aaagtagccc ttggtatgca acaagtagat
    16981 gaacaccact ccgccgacca tcagaagggc aacccacgac atccgacgga aaaaaccggc
    17041 cgggttgcag aaccaaaccg tcagataagc accgacaaac acggtcagcg cgtgctgtcc
    17101 ggacttcgca ccacgcggtc ccaacaaggc atacaggccg cctgcaagga gcgcggcaat
    17161 cagggcggtc cgccaccatc gctgatgcaa tggaaccttc accgcgggct tcacgcgaga
    17221 ttgtgagcgt ttcggtcgtg acatatgccc tcagcatcgg caggcacatg accgaactga
    17281 agggtccgga tatcgtcagc ctttcaccgc gccaagctga atcccgcgca ccaggtaccg
    17341 ctgcagcacg acaaacagca ccatcagcgg cacaatgttc ataactgtcg ctgccatcat
    17401 aagttccgtc tgtcggccat accccgcgcc gccaaggttc tcaaaccaca tcatacccac
    17461 cggcaacgtc cgcaagtgat cgctcttgac catcaccaac ggccagaaaa acgacgcata
    17521 gttgcccagg aaggtcatga tcgccaaaac gataagtccc ggccgcgaaa gaggtaaaat
    17581 cacttcgaca aaaacctgcc accggctcgc cccatcaatc tcggccgctt catccagact
    17641 ggtcggtatg ttcagcatga actggcgaag caggaaggtg ccaaaggcac tgaacgccgc
    17701 cggaaggatc agcccaaggt acgaatccag cagccccagc cgaaacatga tgtaatagtt
    17761 gggaatcagc aagaccaccg ccggaaccat caccgtggcc agataggcca ggaaaaccac
    17821 gtccctgcct cgccacttca gccgcgcaaa cgcgtacgcc gccatcgcac tggtcactac
    17881 ctgcaggaag gtcacccacg aagcgacgaa caggctgttg aaataccacc ggccaaagcg
    17941 gatcggcagc ttttcacccc tgctatcggg cgtgagtccc agcacgatgg ggtagttctc
    18001 ccacttccac tgctccggca ggaactccgg cctgtcggcc tcgtgcggcg gcttgaagct
    18061 cgccagcaac atccacacaa acggcaccgt cattgaagag gccagaccga tcaacaggat
    18121 cgtcctgaga accatccacc ccggcgaccg cccctgcacg gccccgctgc cggctttgcg
    18181 agccagtgga tcctccgcaa gcacgccgat cgaagttgaa ttcatgtctc cctcaaatgt
    18241 tgatctggcg gcttccgaac cgccactgaa ccagcgtcac cgccagaatc atcacaaaca
    18301 tcgtccaagc aatcgccgag gccagcccca gccgaaaatc ggcgaagccc gtcaggtaga
    18361 tgtagaaacc aaggctgatc gttgtttccg ccgggttgcc ctcggtcatg atcttggctt
    18421 gctcaaaccc cccttgtaac ccgccgatga tgctcatgat cacgatgaaa aacgtcgtgg
    18481 gcgccagttg cggccaggaa acatgccgga agctcttcca tcgctcggcg ccgtcgatca
    18541 tcgccgcttc atacaaatcc tgcggcacgt tcgccaaccc cgccaggtaa agcagcatgt
    18601 tgttgccgcc gaccgtggcc cagatgccca tgatcacgat cgcgtcgcga gcgccgagac
    18661 cgaaatgccc cgcggccggc agccccgtct gcggatccag gcaccagaga ttggccgtac
    18721 taccaagcca gttgggtggt tcaatgtcca ggccaagtac ggcaaagagg actcgcaagg
    18781 acgcgttgat cggccccgaa tccggccgga acatcagctt ccacaaaatg tactgcgcga
    18841 cgccggccgt gaagaacggc acgtaaaaca gcgtgcggag gccaacccgg ttgaagaccg
    18901 tggctacggc cgcgctggct ccgatgatcg ccagggccag ccctgtcgtg gcccgtcccg
    18961 tgcccgcaca taccatcaat cccgcgcacg ttgctgcaag gctcgcccca atcaacaacc
    19021 ggcgtactcg tcgcgaaccc aggtagatcg gatcgctcag cagtaccgcc agcaccagcg
    19081 aaccgacaat ggacaccggc atgcccaaca tgaagtaaag ggtcgtcccg aaagctttgc
    19141 ggaagtcctt tgcctcaggc ccgaagaaca atcgctcgaa gttctcaaac cccacaaagc
    19201 caaacggctc ccgctgggcc aggtcccagt tggtgaacgc catcaccagg ctgaacacga
    19261 ccggtgcggc cacgaacacc gcaaaaccca tgatgttcgg caggaggaag agcatcgccc
    19321 acccgaagtc ccgccgtgat cgacgtgaca gccgcaaccc cattagcggt gcccccttaa
    19381 gaccttacgg ccgttcatct gtgcgccttg cgtgcgcgtt tctggatccc ggtgcgcttg
    19441 ggttgcggcc cgcagaaggg cggtccccct tcggcggata gtcggccggg tcgaaatctc
    19501 gccccgttcg ccgcttgtat tcctcgcgca tcgaagcgta gcgggtcaga ctgacccgca
    19561 tttcctcgtt gatctcgtcc gtcagagccc ggcacatctg ttccggggtc gtcttgcggt
    19621 cgctcatcac cgccaagtgc cggttgaata tccgctccac ccgcgtcgtc tgaatgaacg
    19681 gcgggaaatg attgctccgg ccccgcctga tcgcttcgat gaacaggtga ttgaaatcct
    19741 cttccgggtg cgcggcgtcc ttgaggaacc gctcatcctg ccccaccttc ggcgagggcg
    19801 gcaacgcatc tgccgactcg accacctgcc aactgaactc gtccgaagca agaaactgaa
    19861 ggaatcgcag ggcctcgttc ttgttcggac ttttcggatt gatacctacg ctgagcgccc
    19921 tgatctcatc ggctccgctc cgcatgtgtg gcagcaatac ggctccgata tttccccgaa
    19981 ggttcggctg cgtgcgaaac ttgatcatcc cccatcggcc catgcgaagc atcgcgattc
    20041 tttccgtgca gaacaggtcg atcacgctcg atccccaccc gccttgcgtg ccgatcgcgt
    20101 tccg
//
LOCUS       DAOTPW010000092        20100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2737_length_20100_cov_5.703467, whole genome shotgun sequence.
ACCESSION   DAOTPW010000092 DAOTPW010000000
VERSION     DAOTPW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20100
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2737_length_20100_cov_5.703467"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="P5316_10070"
     CDS             <1..661
                     /locus_tag="P5316_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRR85346.1"
                     /translation="DSINVADLAVRLPIVLLALTLHEFSHAYFAYRMGDPTAYRMGRV
                     SLNPLRHLDPLGTLCLLFAPIGWAKPVPVNPLNFDDPRKGDLVTTAAGPGSNLVQALV
                     WALLLRAVLHWWPDGPTSVSGRFELRDVVLLFCLYGTAINVGLAVFNCLPLYPLDGFH
                     ILLQLTKPESQQRLAQTAVYGPFIILGIVLLGRAGEADILSTLINRPTMFVLKHVAGW
                     E"
     gene            688..1668
                     /gene="trpS"
                     /locus_tag="P5316_10075"
     CDS             688..1668
                     /gene="trpS"
                     /locus_tag="P5316_10075"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRR85347.1"
                     /translation="MRLLSGIQPSGRLHLGNYLGAMRPQIEMQDQGHECFYFIANYHA
                     LTSLNDAALVEEYTQGVALGYLALGLDPAKAVFFRQSDVPEVTELAWILSCVTPIGLL
                     ARGHSYKDKVAQGLSPNHGLFAYPVLQAADILIYKAHAVPVGQDQKQHIEMTQDIQAK
                     FNAAFNCDVLIRPEAMIRETVAVIPGVDGRKMSKSYDNTIELFGPEKATRKRIMSILT
                     DSTPVEAPKDPDKCNVLALLKGLASESEIKEWENRYRSGGMGYGEVKKRLADLFFEVL
                     GPARKKHEELLKHPDEVERILAEGGRKARAVAQQTMREVRKACGLKTSGE"
     gene            1715..1921
                     /locus_tag="P5316_10080"
     CDS             1715..1921
                     /locus_tag="P5316_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85348.1"
                     /translation="MGSTCNCGKLFERMMVIGSEDRMVGAQTSPKCRVWRLYRMETAI
                     DPIRLGVWCLARRLAAAGLLLGLL"
     gene            2548..4407
                     /gene="dnaG"
                     /locus_tag="P5316_10085"
     CDS             2548..4407
                     /gene="dnaG"
                     /locus_tag="P5316_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRR85349.1"
                     /translation="MSLADRETITRQIRDATDIVDLVGSSVSLRRSGSAFKGLCPFHE
                     EKTPSFIVNPARQIFKCFGCGVGGDVFKFVQLRENVDFLEARRMLAERAGIDLEAQPR
                     SGVAGLAKTDLARACAWARQIFRKNYLGPSGQAARDYVANRGISAEMAEAFALGLAVD
                     SYDSLIRQADLAKIDHKLLLAAGLVKESQRGGYYDTFRNRLMFPIVDAGGRVVGFGGR
                     TLGEDPAKYLNTPATALFDKGTNLFGLDRARQAIGEAGRAIVVEGYTDCIMAHQHGFC
                     ETVATLGTAMTEAHARLLRRFTDRVILLFDSDEAGQRAADRALAVTVTVGLDVSLARV
                     PEGKDPCDYLLSAGQAAFRGVLNQAVGALEFKWREVSRKYGVGATGPARRRAVEEYLQ
                     QLSAWLGQGAIDPIQTGLLINQLSQIISLPAEDLLQQVRRMSQRTRPLTGRSGTAGRP
                     QAEAPPAGKGSNSQQEALRQIVEVLLNEPAYYAHVASWLKPEHVWDPALSAIVAEMVA
                     ILNSGQQLQVADLIGRFESPEFGGLITDLQDRGERRGQFATVIEGAIACLESFEQSRH
                     AAVLAEDIRRSRQQAGDRQVPENEDERLRALTDAARQSHFSPVRARKRLLDLP"
     gene            4541..6271
                     /gene="rpoD"
                     /locus_tag="P5316_10090"
     CDS             4541..6271
                     /gene="rpoD"
                     /locus_tag="P5316_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRR85350.1"
                     /translation="MAETTISATTDPVELALQSLLERGRERKYITWEEMNEILPDEAV
                     NPDKLEMIMMTLEEHGIEMIDEEEAERLGFTPEAKEAKKPAGKKSQALTPPIDERLLA
                     ALEAEESGSRRIDDPVRMYLTQMGEIPLLTRSQEIALAKKIELTRMAFRKLVLESDYC
                     IAQAVDILQQVSDGRLPFDRTMKVSTSEALAKNTITKLLPENLETVRKLMEANQADFE
                     KTQSGRMAASSKRSIVRAMNQRRRKMAKLLEELSLRTSRIIPLMRKLEGVCRKMMDLE
                     RRIAAEAKRPTLPVEDLAAMEDELAGMESLVLEDSESLQRRLTNLKRVFAEYEDAKRK
                     LSGGNLRLVVSIAKKYRNRGLSFLDIIQEGNTGLMRAVDKYEYKRGYKFSTYATWWIR
                     QAITRAIADHARTIRIPVHMIETMSRLRNISKKLLQELGHEPGIEEIAKRAKMPVSEV
                     RRVLKISRHPISLDRPVGESEDSYFGDFIEDDSVESPVQSAGNEMLRDRIEEVLKTLT
                     YREREILKLRYGIGDGYTYTLEEVGRIFKVTRERVRQVEAKAIRKLQHPVRARKLEGF
                     LENMQETVET"
     gene            6297..7622
                     /gene="hflX"
                     /locus_tag="P5316_10095"
     CDS             6297..7622
                     /gene="hflX"
                     /locus_tag="P5316_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRR85351.1"
                     /translation="MEIDQRANLTVQRERAVLVGLELRGTEHDGVESLRELEALAESA
                     GAIVVDMMLQRRDRVDPKYYIGRGKVDELIGRIEQHDANLVIFDDDLAPAQIRELESA
                     CKVMVIDRSELILDIFASRARTHEARLQVELAQLEYTAPRLRGMWTHLERIAGAAGGT
                     GAGVVGGIGTRGPGERQIEIDRRIVSRRVTQLKRQIAEIERRKVRQVQSREEHFNVSL
                     VGYTNAGKSSLMNALTDAGVVAADRLFATLDTKTRRWKLGGGDEVLLSDTVGFIRRLP
                     HHLVASFRATLEEAINADLLLHVVDASSPSAWEQCQAVTRVLEDLGCSGNRTLVLLNK
                     IDVLWDDSNLRVLQQKLPGALPISAHSGAGLHTLTETVRAMMTGPPQRVTVRLPATDG
                     RAIAFVEKHSQVLDRRYDTQTVEMDVLVGSLILNRLQINFPTTSILRCA"
     gene            8254..9588
                     /locus_tag="P5316_10100"
     CDS             8254..9588
                     /locus_tag="P5316_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85352.1"
                     /translation="MLGVSAVCVFLAVAPAQAVLLSDLVSGAVPSIVIGDKEFTNFSV
                     TITGIGPYTADPSNIDVTGVMSMSGDYGLKFTSANPATAMIWADPVGGFVDVLVGFDA
                     IATGPFTIIGANLSFVGTAPADGLVQIVETVQGDDGQLTLLLVSSLGASSATAILPKP
                     EGYEMVHVVKDIAVVAGGTTRASLDEFTQFFIQIPEPATISLLLLGGLALFGRTLASK
                     RGLLILAMALGLGGMLAQPGTASAVMLSTLTANPANAVTYGDLKFDNFQFSAAGSGKW
                     IADEDAIDVQGFTTPSGSEDGLRFAGLIAALSNVTPGSQVTVTISYDVALTTPGLVFH
                     DVTMSFNGAPTAWDGSAEVSKTIEGIGEVAKVYNRPADPDPIKLIDHQIIGTGNYPGP
                     LHVVDTIVVKGGRDGAAMISYINQTFSLIPEPAMFILTMLGLPLVVGRRRKR"
     gene            9852..10838
                     /locus_tag="P5316_10105"
     CDS             9852..10838
                     /locus_tag="P5316_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRR85353.1"
                     /translation="MNSDCAIGVDLGGQSVKLALVDGRGMVRLRRQQPMDTAKSADQI
                     GRLILDQIRILRREGQLDGLSPSAVGIVMPGYMDRQRTRLLFAANLPTLSGSSLLADL
                     RAGLDLPVTFDADSNGAAFGEYRFGAGRGVNRLTVVAVGTGIGAGVVADGQIVRVRHH
                     IGGSLGHIIVDPRGLRCACGGRGCVETVASGRALEREALQAAQSEPRSLLATLLAQRG
                     SLTGQEVGEALACGDSAALRIVRECGWWLGVALASWAVVHAPQKVLIGGGIAQLGQPF
                     IQAIHDGLQEVGQPTLVRDTAVELAGLGSDAGVVGAAGLALADLAETRPASG"
     gene            11082..11879
                     /locus_tag="P5316_10110"
     CDS             11082..11879
                     /locus_tag="P5316_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85354.1"
                     /translation="MSVASERSNRDRAGFTLIELLVVVAILALLISILIPSLARAREQ
                     SRRAVCASNLHQQILGMYSYASDHRNFLPWRGWFSYDISEAHAEAYGGGGNKKVLVNL
                     ALLIGKHLGKDWDVLYCPCTLATYRDSPSGLSSLMDPDYRFSHGGYNYALPIGKRVGA
                     PKLDLDVYPRDAAKLDSRWLDVLKENAPPEYIDSAGNVDLIKMMPRRIQPIVMDFVAG
                     GGKTLHPNGLNVSFSDGHARFVPYKMPSGSVDNVTSFSLWYHISARP"
     gene            12258..13526
                     /locus_tag="P5316_10115"
     CDS             12258..13526
                     /locus_tag="P5316_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85355.1"
                     /translation="MRMMFILSVVSVVIPASGVGSVLGAPMLQSIGVLDPANPASAVT
                     AISSDGTWAVGHSNGLDSAGTTSIRQPIIWSQSTGLVQLPNTGDFNGEARGVAVKPDG
                     YIGIAGYVYNTTLTTPLPKMHGYKALPSNLKGGTWTLCSGASTTGNDTVGPYNAARVK
                     WLTDGTVTGDYLISGRRGNGARGIAVTMDANTYIDYPNGTPRVVANSVASRLVNNSRP
                     FGAGYDNGNPNGLRRALLVYTTSAPQLTIPGGVGYQSEALGVSPDGLFVAPGDASNAG
                     VLCGYDQDVSGLPHAFIWKINDPAMTILGEAMGDNQSTAIDVRVIGGNWTAVGYSSDG
                     ITERAVMWLNGSMQPVLLSSLLAGAGVDTSGWASLSRITSMSDDGMTVAGWGIWAEDG
                     STRGFVAVIPEPAALSLLVLGGCALLRRRQ"
     gene            complement(13836..16697)
                     /locus_tag="P5316_10120"
     CDS             complement(13836..16697)
                     /locus_tag="P5316_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85356.1"
                     /translation="MSSTPNICSRLAGVFAATALLVPTNGLLAQFRNEQQPFEGTSNT
                     FFAIPEGSVPYWSVDGACSPALLFKDGTRGDNGSSCLRSLLPVGTGTRMILDLRPGPD
                     GSTIGDMKAISFDVITRLFVSADDGSGVAGQELYRVLQPLYPVMTLELDIDPGTPGSN
                     PSNMFAQYDKLYFAADDGTNGVELWKATLRNNLTVATALLKDINPLAGSSSPAGFALL
                     SGSVVMFSADDGTTGRELWKTDGTNTTRQLDINQVVDPNDASKTLSSDPQLLTTVSGK
                     VFFTADDGVNGRELWMTDGTTTMMVLDIVSGAAGSNPTNLTAATFLVGGAPTPMLCFT
                     VDSDGMNGAELWKSNGTAAGTMMIYDPGSAGSPANLRSIAGTVNRVFFTLGNDLWKSD
                     GTMVGTVPVKTFVSAPTNLVVAANTLYFVADDNSGGGLELWKSDGTTTSQVMDIRPGA
                     VGSSPQNLFAIGSTVYFSADDGTYGRELWRTDSAAGALRVMDINPGPADSAPSNMANF
                     DGTLVFTADDGTHGRELWQSDGFPVANRVGMSYKWDLATLISNKGDPGKMAFAVDNTK
                     PLYIYWLHDFPAMGGPSGVSEGAHEINTYVELTDGTDRAPLNITNVDCGDGNLPRPRA
                     ALTDGTNHNAIAFGMVAVLDQDPCDTDSRGPGSPSTPFAYVPAVYDGLNWIPMDMAHI
                     TPAVPTAPPSGTALGQTSSFAYLWTGMPSNLVPATVDPWSTKRFTFARLDVWSDYLTV
                     MWGGKQNGTIWVATVPRQYKGGFSALYFGNKGCVQPAYPFYLDSLTLNGGVFTSSPHP
                     VGACCLPDSCVDGVISDTACAALGGSWAGFDTTCDGSACLGACCRPMAVCTETLAGAC
                     DGDFLGVGTNCATATCPCHSPRADADWDGDVDQVDFSALQGCYSGPGPLPDPLPAGCK
                     CFDRDLTGAADGDVDADDYDAFEKCASGPGVPLDPACEL"
     gene            17083..18219
                     /locus_tag="P5316_10125"
     CDS             17083..18219
                     /locus_tag="P5316_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HRR85357.1"
                     /translation="MDVEAPRGLLRFFDELEDPRMERTKLHSLEDILFIALCAVICGA
                     DSWTEVEVFGWAKREWLSQYLTLPNGIPSHDTFGRVFSRLDPQVLERCFSKWMAALAE
                     ASAGRLVAIDGKTLRRSFDKATNRAAIHMVSAWCETNRLVLGQLATAEKSNEIKAIPQ
                     LLKMLDIRDAVVTIDAMGCQKAIAQEIVQQGGHYLLQVKDNQPGLHDVVKATFDELTG
                     RGIAGVQYDFHEQTDAGHGRIETRRLWVTDWTDWYADRKEWANLTSFVCMESVRTVNG
                     STSCERHYYISDLAGQSAQTMLGYVRGHWGIENKLHWSLDMTFREDTLRNRIGHSAEN
                     LSRIRRLSLNLLCREKTCKASLKSKRLRAGLREDYLVRVLCQGI"
     gene            complement(18776..19903)
                     /locus_tag="P5316_10130"
     CDS             complement(18776..19903)
                     /locus_tag="P5316_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85358.1"
                     /translation="MQETTRTVNVAFSRRRLLRLAGAGAATLAAARTTLGQETVGGNN
                     IEFEKTLFAMRDRNYRHRKVDIVKTGFRPVGGNVADFGVADFKGRLHFFYIERRLQEG
                     TPFYPGHEIFFGHASTSDFVNWEVHDPVMLIRPGTWEEAHVWAPCVIRRGDEFVMAYT
                     GLNRHISQNIGLASSKDLFEWRRWDSNPISPCKGKEWAFWREDMISSCRDPSICEHDG
                     RYWMIFTANTRQGESCIAMVSTTDFKRWQDHGPICVGPATGYEPRLEGGHPQGSLESS
                     NLFFRKGRWFLTVKAKIRNEKAVNWIIENDTNLKFDFAKRREFWPGATGIEIARTRGD
                     RDLLATFTAGHIKFGLTDWSHEIPTGRFIESEDELTEWKME"
BASE COUNT         3927 a         5680 c         6457 g         4036 t
ORIGIN      
        1 cgattccatc aatgttgctg atttagccgt ccggttgccg atcgtgcttc tggctctcac
       61 gttgcacgag ttttcccacg cgtactttgc ctaccgcatg ggggacccga cggcttacag
      121 gatgggccga gtcagtctca acccgttgcg gcatctggac cccttgggca ctctctgtct
      181 gctgttcgct ccgatcggct gggccaagcc ggtgccggtc aacccgctga acttcgacga
      241 tccgagaaaa ggtgatcttg tgacgacggc ggccgggccg gggagcaacc tcgtgcaagc
      301 ccttgtgtgg gccctcctgc tccgtgctgt tctccactgg tggccggacg gcccgacgtc
      361 cgtgagcggc aggtttgagc ttcgggacgt cgttcttctc ttttgccttt acggaaccgc
      421 cattaacgtc ggcttggcgg tgttcaattg tcttccactc tacccccttg acggctttca
      481 tattctcctc caactgacca agcccgagtc acagcaacgg ctcgcccaga ccgccgtgta
      541 cggtcccttt atcatcctgg gcatcgtgtt gctgggacgc gccggcgaag ccgatatact
      601 gtcgacttta ataaaccgac cgacgatgtt cgtgctcaag cacgtcgccg gctgggagtg
      661 actaaactaa aagacgggtt tgtgattatg agactgcttt ctgggatcca accatccgga
      721 agactgcacc tgggcaacta tctcggagcc atgcgtccgc agatcgagat gcaagaccag
      781 ggccatgaat gtttctattt cattgccaat tatcatgcgc tgaccagcct gaacgacgcc
      841 gctttggttg aggaatacac gcaaggggtt gctctgggat atctggcgct cgggttggac
      901 cctgccaagg ccgtgttctt ccgtcagtcg gacgtgcctg aggtgaccga actggcttgg
      961 attctctcgt gcgtcacgcc catcggcttg ctggcccgcg ggcattcgta caaggacaag
     1021 gtggctcagg ggctttcgcc caatcatggc ctgtttgcct acccggtgct ccaggcggcc
     1081 gacattctca tctacaaagc ccacgccgtg cccgtcgggc aggaccagaa acaacacatc
     1141 gaaatgactc aggatattca ggccaagttc aacgcggcat tcaattgcga cgtcctgatc
     1201 cggccggaag cgatgattcg cgaaacggtg gccgtgatcc ccggcgtcga cggccgcaaa
     1261 atgagcaaga gctacgacaa caccatcgag ctgttcggac cggagaaggc cacgcgcaag
     1321 aggatcatgt ccatcctgac cgactcgacg cccgtcgagg cccccaagga tccggacaaa
     1381 tgcaacgttc tggcgttgct gaagggattg gcgagtgaga gtgagatcaa ggagtgggag
     1441 aaccgttacc gctccggagg catgggctac ggcgaggtca agaaacgcct ggccgacttg
     1501 ttcttcgagg tcctcggccc tgcccggaag aaacacgaag agctgctcaa acacccggac
     1561 gaagtcgagc gcatcctggc cgaaggcggc cgaaaagctc gtgccgttgc tcagcaaacc
     1621 atgcgcgagg tccgaaaagc gtgcgggctg aagacgagcg gggagtagag gtgcagtgag
     1681 cccattccgg gcatcagtgc gtcagccgtc gcgcgtggga tcgacttgta attgtggcaa
     1741 gctctttgag cgaatgatgg tgatcggcag tgaggaccgc atggtaggcg cccagacctc
     1801 gccgaagtgt cgcgtctggc gactctaccg catggagacc gccatagatc ccatccgcct
     1861 cggcgtctgg tgcctggcgc gcagactcgc ggccgctggt ctgcttctcg gcctgctgtg
     1921 aagcctcaac ggcggcggag gaggggcggc ggcgcgggag gcaacaacgg gacaagcctt
     1981 ctcgttgaca acgcaggcgt cgctttgtgg cgactggttg tggccttggg cttcgccttc
     2041 tctaaggtgg accgcctttt tgggggccgt aacccaagcg ctctgctatc atgcgcgcaa
     2101 gccgcacaga tgtctggccg taaggttctg acacctgggt gtggggaggc cctcgaatcc
     2161 gtggccgcgt ggggtcatcg gcacgtggcc ctctcggtca aacgcgggcg gcacattcgg
     2221 tcaccagcct gtcaacgtcc cgtaaccgct cgcccaccgg acactttggc cggagggaac
     2281 ctctgagagt ccaggcgatt tttcaaggaa tcagcccgtt tctggcacgc ttcttgcacg
     2341 ggatgcaatc cagccgtaac caccgaaaaa ataaaactct aagaccattt gccagacccg
     2401 aaaagatggt gtatagtttt cggtctccca aagagcgagg taggcacgac gggtgtgatg
     2461 agatagcgtt taaggttttg ccaaatctag gcttaggcct gttctcgcaa tatccgggtt
     2521 ttattgggat gttccagagg gcaagcgttg tctttagctg acagggaaac cattacccgg
     2581 caaatccggg atgccaccga catcgtggat ctggtgggga gtagcgtgtc gctgcggcgg
     2641 tcggggtctg ccttcaaagg actttgccct tttcatgagg agaaaacccc atccttcatc
     2701 gtgaacccgg cgaggcagat cttcaaatgc ttcggctgcg gtgttggagg cgacgtcttc
     2761 aagttcgtcc aactgcggga aaacgtggac tttcttgagg ctcgccgcat gttggccgag
     2821 agagcgggga ttgaccttga ggctcagccc agatccgggg tggccggcct tgccaaaacc
     2881 gatctggctc gggcctgtgc ctgggcccgg cagatctttc gcaagaatta cctggggcca
     2941 agtggccaag ctgccaggga ttacgtagcc aaccggggta tctccgcaga gatggccgaa
     3001 gctttcgcgc tgggcttggc ggttgacagt tatgattctc taatccggca ggccgatctg
     3061 gccaagatcg atcataagtt gttgctggca gcaggtttag tgaaggagag ccagcgcggc
     3121 ggttattacg acaccttccg caaccgtctc atgttcccga tcgtggatgc cggcggtcgc
     3181 gtggtcggtt tcggcggccg aacgctgggt gaagatcctg cgaagtatct taatacaccg
     3241 gctacagcgc tgttcgataa agggacaaac ctcttcgggc tggatcgcgc tcgccaagcg
     3301 attggtgagg cagggcgagc cattgtcgtg gagggctaca cggactgcat catggcgcac
     3361 cagcacggct tctgcgagac agtggccacg ctggggacgg ccatgacgga ggcccacgcc
     3421 aggctgctgc gccgcttcac ggatcgcgtc attctgctat ttgactcgga cgaggccggg
     3481 cagcgggccg cggacagggc cctggcggtg accgtgaccg tgggtctcga cgtctcgctg
     3541 gcgcgcgtgc ctgaaggcaa agacccctgc gattatcttt tatcggccgg tcaagcagct
     3601 tttcgtgggg tgttgaacca ggccgtcgga gcgctagaat tcaaatggcg ggaggtgtcg
     3661 cgcaaatatg gtgtcggcgc gacaggcccg gcacgcagac gggcggtgga ggagtacctt
     3721 cagcaactgt ctgcgtggct gggacagggg gcaattgatc cgatccagac ggggttgctg
     3781 atcaatcagt tgagccagat catctcgcta ccggcggagg atctgcttca gcaggtgcgc
     3841 aggatgtcgc agcggacgag gcctttgacg ggccgcagtg gaacggccgg tcgaccgcag
     3901 gcggaagcgc cccctgccgg gaaggggtcc aacagccaac aggaagccct tcgacagatc
     3961 gtcgaggtgc ttctgaacga gccggcatac tacgcgcacg tggcatcgtg gctaaagccc
     4021 gagcacgtct gggacccggc tctctcggcg atcgtcgcgg agatggtggc gatattgaat
     4081 tccggtcagc agttgcaggt tgccgacttg atcgggcgct ttgaatcccc tgagttcggc
     4141 ggcctgatca cggacctgca ggaccgtggc gaacgacgcg ggcaatttgc caccgtgatt
     4201 gaaggggcca tagcctgcct ggagtcgttt gagcagtcgc ggcacgcggc cgtcctggcg
     4261 gaagatatcc gccgttcgcg gcaacaggcc ggcgaccggc aggttccgga aaacgaagac
     4321 gagcgactga gagcgttgac ggacgcggcc agacagtctc atttctcgcc cgtacgagcc
     4381 aggaagagac tgttggacct accgtgaagg acggagaagg aggccctcgc cagtaaggcg
     4441 taatcgagcg gcgaggaggc agcgtggcaa ggaggcccgc tgcgtcaaga atcagacgga
     4501 tgaacaagcc aggcaggata gttgacctgc ggagttgaca atggcagaga cgaccatttc
     4561 agcaacgacg gatcccgttg aactcgcgct tcagtcgctg ctcgaacgcg gccgggagcg
     4621 aaagtacatc acctgggagg agatgaatga gattctcccg gacgaggccg tcaatccgga
     4681 caagctcgag atgatcatga tgactctcga ggagcacggc attgagatga tcgacgagga
     4741 ggaggccgag cggctgggtt ttactcctga agccaaggag gcgaagaaac ccgccggaaa
     4801 gaagtcccag gcgctgacgc cgccgatcga tgagcggctg ttggccgccc tcgaggccga
     4861 ggagagcggt tcccgacgca tcgacgatcc cgtgcgcatg tacctcaccc agatgggcga
     4921 gattcccttg ttgacccgct cgcaggaaat cgccctggcc aagaagatcg agctgacacg
     4981 aatggccttc cgcaagctgg ttctcgagag cgactactgt atcgctcagg ccgtggacat
     5041 cctgcagcag gtttccgacg gccgattgcc gtttgaccgg accatgaagg tgtcgacgtc
     5101 cgaggccctt gccaagaaca ccatcaccaa gctgttgccg gagaaccttg agaccgtccg
     5161 gaagctcatg gaggccaacc aggccgattt cgagaagacg cagtccggtc ggatggccgc
     5221 gtcgagcaag cgcagtatcg tgagggccat gaaccagcga cgccgcaaga tggccaagct
     5281 gctggaggaa ctgtccctgc ggaccagccg catcatcccg cttatgcgaa agctcgaagg
     5341 ggtctgtcgc aaaatgatgg atctggaacg gcgcatcgcg gccgaggcca agcgtcccac
     5401 tctgccggtg gaagacctgg cggccatgga agacgagctt gcgggcatgg aatccctggt
     5461 acttgaagac agcgagtcgc tccagaggcg tctgacgaac ctcaaacggg tttttgccga
     5521 atacgaagac gccaagcgca aactgtccgg cggcaactta cgcctggtgg tgtcgatcgc
     5581 caagaagtac cggaaccgcg ggttgagttt cctggacatc atccaggagg gcaacaccgg
     5641 cctgatgcgg gcggtggaca agtatgagta caagcgcggt tacaagttca gcacctacgc
     5701 cacgtggtgg atccggcaag cgatcacccg ggctatcgcc gaccacgccc ggaccatccg
     5761 catcccggtc cacatgatcg agaccatgag ccggctgagg aacatttcca agaagcttct
     5821 ccaggagctc ggccatgaac ccggcatcga ggaaatcgcc aagcgggcca agatgcccgt
     5881 cagcgaggtt cgtcgcgtgc tcaagatcag ccgccacccg atctcgctgg atcgtcccgt
     5941 cggggaaagc gaagactcgt acttcggcga tttcatcgag gacgattcgg tcgaatcgcc
     6001 ggtgcagtcg gccgggaacg agatgcttcg cgaccggatc gaggaggtgc tcaagactct
     6061 cacctaccgc gagcgagaaa tcctgaagct tcggtatgga atcggagacg gatacaccta
     6121 cacccttgag gaggtcggca ggatctttaa ggtgacccgc gaacgggtgc gacaggtgga
     6181 agccaaggcg atccgcaagc tgcagcatcc tgtgcgggct cgcaaactcg agggcttcct
     6241 tgagaacatg caggaaacgg tggaaacgta gggggccaca cgagacttca atcaccttgg
     6301 aaattgatca acgagccaac ttaacggttc agcgggagcg ggcagtcctg gtcgggctgg
     6361 agttgcgcgg aacggagcac gacggggtgg aatccctgcg tgagctggag gccttggccg
     6421 agtccgccgg cgcaattgtt gtggacatga tgctccagcg acgcgaccgc gtcgacccga
     6481 agtactatat cggcagaggc aaagtcgatg aactgatagg ccgcatcgag cagcacgacg
     6541 ccaacctggt gatcttcgac gacgacctgg ccccggccca gatccgcgag ttggagtcgg
     6601 cctgcaaggt gatggtcatc gaccgcagcg agctgattct cgacattttt gcctcgcggg
     6661 cccggaccca tgaggcccgg cttcaggtgg agctcgccca gctcgagtat accgccccac
     6721 gtctgcgggg catgtggacg cacctggaga ggattgccgg ggccgccggg ggcactggtg
     6781 ccggcgtggt cggcggcata ggtacccgcg ggccgggcga acgccagatc gaaatcgacc
     6841 gccgcatcgt ttcgcgacgc gtcacgcaac tcaaacggca gatcgccgaa attgaacgcc
     6901 gcaaagtccg ccaggtgcaa tcgcgggagg agcatttcaa cgtatctttg gtgggctaca
     6961 ccaacgcggg gaaaagctcg ttgatgaatg cccttacgga cgccggcgtc gtggctgccg
     7021 atcgtctctt cgccacgctc gacaccaaaa cacgccggtg gaaactcggt ggcggcgacg
     7081 aggttctgct cagcgacacc gtcggcttta ttcgacgcct gccgcatcac cttgtggctt
     7141 cttttcgcgc gacgcttgag gaggcaatca atgccgatct gctgcttcac gtggtcgacg
     7201 cctcgtcgcc ttcggcgtgg gaacagtgcc aggcggtaac aagggtattg gaggatctgg
     7261 ggtgttcagg caaccgcacc ctggtattgc tcaataagat tgacgtgctt tgggatgaca
     7321 gcaatctgcg cgtgctccag cagaagttgc cgggggcact gccgatctca gcgcacagcg
     7381 gcgctggtct tcatacactc accgagacgg tccgcgcgat gatgaccgga cctccgcaac
     7441 gagtcaccgt ccggttgccg gcgaccgacg gtcgggcgat cgcgtttgtt gaaaagcact
     7501 cacaggttct cgatcgacgt tacgacactc agacggtgga gatggacgtt ctggtcggca
     7561 gcctgatcct gaatcgtctg cagatcaact tcccaacgac gagcattctg cgttgcgcct
     7621 gacgtgtgaa gtcatttgtc cggggacatc cggtcgaaga gccgcaactc gcgaaaggag
     7681 tcttctggta tgttctgtcc agcttgccgc aagttggagt gaacgtttca ggggcagcct
     7741 tgcaagcgaa gtggtggccg ataaagcgcc ggttgcagtc attggcataa aaaggtcaat
     7801 ttctggattg gcgacacctc gttgggaggg ttttcggcga tcgcttgccg aggagtattg
     7861 aacgtccgat attgtgcgct gtaagccttt gcgaagaaat gctttacaat tcctggcgtt
     7921 caggttgctg aactacaagc taatccgaaa aaaactttca ggagccgttt tttctcttga
     7981 tgccgatggg gcggggcggt agaatacgtt gcatgtggag gacgtgcgcg atcgtccggc
     8041 gccagtcggt acatcgtgca caggcgggag agagagaaaa ttccttctag ggaaatcccg
     8101 ccgaacaaga tggagcgaga gagagggctc gttttcgttc gtatttgatg aaggctcagt
     8161 acgaaattcg aaactcgagc gtgttgggag agacagctct ttttgggaaa gggagagtga
     8221 gagatgagag gaagcctacg cacgtcgaag gcgttgctgg gagtctcggc cgtctgtgta
     8281 tttctggccg ttgcacccgc acaagctgtg ttactttctg atttggtcag cggtgccgtg
     8341 ccaagcatcg tcatcggtga caaagagttc accaacttca gcgtgacgat tacaggcatc
     8401 gggccgtata cggccgaccc ctctaatatt gatgtcaccg gcgtgatgag catgtccggc
     8461 gattacggcc tgaagttcac gagcgcgaat ccggcaacgg cgatgatctg ggccgatccc
     8521 gtcggcggct tcgttgacgt actggtgggc ttcgatgcaa tcgctaccgg cccgtttacg
     8581 atcataggtg ctaatctttc gtttgttggc actgcccctg ccgatggtct ggttcaaatc
     8641 gtcgagacgg tccaaggtga cgatggtcaa ctgactctgt tgctggtgag ctcgctgggc
     8701 gccagttcgg ccacggctat tcttccgaaa ccggagggat acgagatggt tcatgtggta
     8761 aaggatattg ccgtggttgc tggtgggacc acacgagcgt cgttggacga gttcactcag
     8821 ttcttcatcc agattccgga gccggcgacg atctcgttgc tgctattggg cgggctcgcg
     8881 ctgttcggcc gcactctggc aagcaagcgc ggtctgttga tcctcgccat ggcattgggg
     8941 cttggcggca tgctcgcaca gcccggcact gcttcggcgg tcatgctgtc cacacttacc
     9001 gccaaccccg cgaacgccgt gacctacggc gacctgaaat tcgacaattt ccagttcagt
     9061 gcggcgggaa gcgggaaatg gattgctgac gaggatgcca tcgacgtcca gggcttcacc
     9121 acgcccagcg gcagcgagga tggcctgcgt tttgccggcc tgatcgccgc gctgtcgaat
     9181 gtcaccccgg gctcccaggt aaccgtcacg atttcgtacg acgtggcgct caccaccccg
     9241 gggctcgtat tccacgacgt gaccatgtcc tttaacgggg ctccaactgc gtgggacggc
     9301 tcggccgagg tgagcaagac gatcgaaggg atcggcgagg tggccaaggt ttacaatcgg
     9361 ccagctgacc cggacccgat caagctgatc gatcatcaga tcatcggcac aggtaactat
     9421 cccggtccgc tgcacgttgt tgatacgatc gtcgtgaaag gcggtcgcga tggtgcggcg
     9481 atgatttcct acattaacca gacgttttct ctgattcccg agcctgccat gttcattctg
     9541 accatgctcg gattgccgct ggttgttggc cggaggcgga aacggtaggc acagaccctc
     9601 gcggcgggtc ccaagagact ctctcgacat gatgagcggc cgaccgctat ggtcggccgc
     9661 tctttttcat tcatccttct gcttgacgga attccttttc gccgtcagat tgattgcagc
     9721 gggaaccgga ctgttttcca ggattcgacc ttcattcgta agggcggcgt tgcgagagtt
     9781 ggtcggtctg gagggcgacc gccgcgtttt tcctggaacg taccgataca agaaaggacg
     9841 acgaccccgt tatgaatagc gactgcgcca tcggcgtaga tcttggaggc cagagcgtca
     9901 aactcgcgct ggtcgatgga cgcggcatgg tccggcttag acggcagcag ccgatggaca
     9961 ccgcgaagtc ggcagaccag atcggcagac ttatcctgga ccagatccgg attcttcgtc
    10021 gtgagggcca attggacggc ctttcgccct cagcggtcgg cattgttatg ccgggctata
    10081 tggaccgtca gagaacccgg ctgcttttcg ccgcgaacct gcccaccctc agcggttcga
    10141 gcctgctggc cgacttgcgg gccgggctgg acttgccggt gaccttcgac gccgactcga
    10201 acggcgcggc gttcggcgag taccgtttcg gggccgggcg gggggtgaac cggctcaccg
    10261 tcgtggccgt cggcacaggc atcggggcgg gcgttgtggc cgatggccag attgtccggg
    10321 tccggcacca cattggcggg agcctggggc acatcattgt cgacccacga gggttaagat
    10381 gtgcttgcgg cggtcggggt tgcgtcgaga cggttgcctc cggccgagcc ctcgaacgtg
    10441 aggctttgca ggccgcccaa tccgaacctc gttctctgct ggcaaccctg ctggcccagc
    10501 ggggcagcct cacaggccaa gaggtcggcg aggccctggc ctgcggcgat tcggcggcct
    10561 tgcggatcgt ccgcgagtgc gggtggtggt tgggggtggc cttggcaagc tgggcggtgg
    10621 ttcatgctcc ccagaaggtc ctgatcgggg gcgggatagc ccaactcggg caacctttca
    10681 tacaggcaat ccacgacgga ttacaggaag tcggacagcc gacactggtt agggacacag
    10741 cagtcgaatt ggccggtctg ggttcggatg ccggcgtagt tggagcagcg gggctggcct
    10801 tggcagatct ggctgagacg cggccggcct ccggctgagg tcaggttgat cgaatcgact
    10861 gggctgacgt caggatcacc gataaatctc atcggaaggc tctggcgaac ttgcctttct
    10921 gtaaaccata gtgtagatta tatgtagacc agtcggcccg taaacggccg gtcttggagc
    10981 ccctttctgg gcatgcaagg cgttcggacc aatcgccacg aaggtgacgg ttgccgaaga
    11041 ggagcaattg tgtcgtcgcc tcgcgaagcg ggagaaccat catgtcggtc gcttcggaaa
    11101 gatcaaaccg tgatcgcgcg ggtttcacgt tgattgagct gctggtggtc gttgcgattc
    11161 ttgcccttct gatcagcatt ctgattccct cgctcgcgcg ggctcgcgag cagtccaggc
    11221 gagccgtttg tgcgtcgaat cttcaccaac agattctggg catgtattcg tacgcttcgg
    11281 accaccggaa cttcctgccg tggcgcggat ggttctcgta cgacatttcc gaagcgcacg
    11341 cggaagccta cggtggcggc ggaaacaaga aagttctggt gaatctcgcg ctcctgatcg
    11401 gcaagcacct ggggaaggat tgggacgtcc tttactgccc atgcacgctg gccacctacc
    11461 gcgactcacc atcggggctc tcgtcgctga tggatccgga ctaccgtttc tcgcacggcg
    11521 ggtacaacta cgccttgccg atcggcaaac gcgtgggtgc cccgaaactc gatctggacg
    11581 tatacccccg tgacgccgcc aagctggaca gcagatggct ggacgttctg aaggaaaacg
    11641 cgccgccgga gtacattgat tccgcaggca atgtcgatct gatcaaaatg atgccgcggc
    11701 ggattcagcc gatcgtcatg gactttgtcg ccggcggggg gaagaccctg catcccaacg
    11761 gtcttaacgt ttcgttcagc gacggtcacg cccggttcgt tccttacaag atgccgagcg
    11821 ggtcggttga caacgtaaca tctttctcgc tctggtacca catcagcgcc cgcccgtaag
    11881 ggtgcttatt cacaagtggg actttttgca cattctcgga gcctgcggtc gagaagaacc
    11941 ttgacagcgg catcggcgga ctgatactat aggttcatca gatctgctct ttcagaattc
    12001 cccgccgtat ccgttcgacg aggtgggtag gttagggcat atgggaggtg aaggggcgcc
    12061 agcacacaca atcgcggcat ggcttgccga ggggcgcctc atcagggagt tgtccggtca
    12121 actcgaattc tcgcggttgt tttgcgttgc gtgtggagag cgggccgttg aaccgagtga
    12181 catagttcgg cgggagagaa cgggagacga aatcatttgt actggtccgg gagaagcccg
    12241 ggaggaggac aattgccatg agaatgatgt tcatattgtc agtggtctcg gtggttattc
    12301 cggcgtcggg ggtaggcagc gtcctcggcg cccccatgct gcagtccatc ggcgtactgg
    12361 atccggcaaa ccccgccagc gcggtcaccg ccatctcgtc ggacggtacc tgggccgtag
    12421 gccacagcaa tggcttggac agtgcgggca cgacctcgat tcgacagccg atcatctggt
    12481 cgcagagcac gggcctcgtg cagttgccga ataccggcga cttcaatggc gaggcacgag
    12541 gcgtcgcggt caaacctgat ggctacatcg gcatcgccgg gtatgtgtac aacacgactc
    12601 tgaccacgcc cctgcccaag atgcatgggt acaaagcctt gccatccaat cttaagggcg
    12661 gaacgtggac cctttgttcc ggggcgtcga caaccggcaa cgacacggtc ggcccttata
    12721 acgcagcacg tgtgaagtgg ttgacggatg ggacggtcac cggcgattac ctgatatccg
    12781 gaaggagagg gaacggcgcg aggggaatcg cggtgacgat ggatgcgaac acctatatcg
    12841 actatcccaa cggcacgccg agggtggtag ccaacagcgt cgcgagcagg ctggtgaaca
    12901 acagtcgccc ctttggcgcc ggctatgaca acggcaatcc gaacggtctg cgccgcgcgc
    12961 ttcttgtgta cacgacttcc gcgccgcagc tgaccatacc gggaggcgtg ggttatcaga
    13021 gcgaggcttt gggcgtctca cccgacggcc tgtttgtcgc gcctggcgac gcctcaaacg
    13081 ccggggtgct ctgtggctat gatcaggacg tatcgggtct cccacacgct ttcatctgga
    13141 agatcaacga tcctgccatg accattctgg gtgaggccat gggagacaac cagagcacgg
    13201 cgatcgatgt ccgagtcatc ggcgggaact ggactgcggt gggctattcg tcggatggca
    13261 tcaccgaaag ggccgtgatg tggttgaatg gaagcatgca gccggtgctg ctatccagcc
    13321 tcttggccgg cgccggtgtg gataccagcg gttgggccag cctctcgcgg atcacaagca
    13381 tgtcggacga cggcatgacg gtcgccggat ggggcatctg ggcagaggac gggtccacac
    13441 ggggctttgt cgcggtcatt ccggagccgg cagcgctgag cctgctggtt ctgggggggt
    13501 gcgctctgct ccgtcgcagg caataaacac ctttaggagc ataaaacagc attaaaccat
    13561 tcctgccgcc gggcctgtat tgcggaggta tgcgatgccg cccgcggtat acggtttccg
    13621 cccctttctt tcgggggtgg aggcgttacg ggggatatgg tttcatcgtt tcattccggt
    13681 tcatacctgt tgccgcaggc atgacgcgta cgcggcccta ttggctgtcc cagcacgggc
    13741 acgagagcgg actgcgaggc tccggtcggc aaacgagcag gccggccggc gagactcctc
    13801 ccggcctgct tgtatcggga gcgcggaatg cgggctcaca gctcgcatgc aggatcgagc
    13861 gggactccgg ggccgctggc gcacttctcg aaagcatcat agtcgtccgc gtcgacgtcg
    13921 ccgtcggcgg caccagtgag gtcgcggtcg aagcacttgc agcccgctgg gagcgggtcc
    13981 ggcaggggac ccggtccgct gtaacagcct tggagagcgg agaagtcgac ctgatcgaca
    14041 tcgccgtccc aatccgcatc tgcgcgagga ctatggcatg ggcaagtggc ggtcgcgcaa
    14101 ttcgtgccga cgccaaggaa atcgccgtcg catgcgccgg ccaaggtttc ggtgcacaca
    14161 gccattggcc ggcagcacgc gcccaagcag gccgagccgt cgcacgtggt atcgaacccg
    14221 gcccaagagc cgcccaacgc cgcgcaagcc gtatcagaga tgacgccatc cacgcacgag
    14281 tccggcaggc agcaggcccc gacggggtgg ggcgagctgg tgaacactcc gccgttcaag
    14341 gtcaggctgt caagatagaa gggataggca ggctgcacac agcctttgtt cccgaagtaa
    14401 agagcgctga atccgccttt gtactgacgg gggaccgtgg cgacccagat ggtgccgttc
    14461 tgctttccgc cccacatgac cgtaaggtag tcggaccaca catcgagtcg ggcgaaggtg
    14521 aagcgttttg ttgaccatgg atccacggtt gcaggcacga ggttactcgg cataccggtc
    14581 cacagatatg caaaggacga tgtctgtccc agggcggttc cggagggagg agcagtagga
    14641 accgcggggg tgatgtgagc catgtccatc ggaatccagt tcagaccgtc gtagactgcc
    14701 ggcacgtagg cgaacggtgt gctgggactg cccggtccgc gtgaatcggt gtcgcaggga
    14761 tcctgatcca gtacggccac cattccgaag gcgatggcgt tgtggtttgt tccgtcggtc
    14821 aaagcggctc tcggtcgggg aaggttaccg tcgccgcagt ccacattggt gatgttgagc
    14881 ggagcgcggt cggtaccatc ggtgagttcg acgtaagtat tgatctcatg cgccccttcc
    14941 gagaccccgc tgggaccgcc cattgccggg aaatcgtgca gccagtatat atacaggggc
    15001 ttcgtgttat cgaccgcgaa cgccatcttg ccggggtcgc ccttgtttga aatcagtgtg
    15061 gcgaggtccc acttgtagct catgccaaca cgattggcca cggggaaccc gtcgctctgc
    15121 cacaactcgc gtccgtgggt tccgtcgtcg gccgtgaaga caagggtccc gtcgaagttg
    15181 gccatgttgg agggggcgga atcggcgggt ccgggattga tgtccatgac ccgcagcgcg
    15241 cccgcggcgc tgtcggtacg ccacaattcg cggccgtacg taccgtcgtc ggcggagaaa
    15301 tagaccgtgc tgccgatggc gaacaggttc tgcggcgaag aaccgacagc gccgggtcga
    15361 atgtccatga cctgtgacgt agtcgtgccg tcgctcttcc acagctcgag accgccgccg
    15421 gagttgtcat ccgcgacaaa gtagagggtg tttgccgcga cgacgaggtt tgtcggggca
    15481 gatacgaagg tcttaaccgg tacggtgccg accatggtgc cgtcgctttt ccagaggtcg
    15541 tttccaaggg taaagaacac acggttaacc gtgccggcaa tgcttctcaa gttggccggg
    15601 cttcctgccg atccagggtc gtaaatcatc atcgtgccgg cagcagtccc gttactcttc
    15661 cagagctccg caccgttcat gccgtcacta tccacggtga aacagagcat gggtgtaggt
    15721 gcgccgccga ccaagaaggt tgccgccgtg aggttggtcg gattggaccc ggccgctccg
    15781 gacacgatgt ccaacaccat catggtggtt gtaccgtcgg tcatccacaa ctcccgtccg
    15841 ttgacgccgt cgtcggccgt gaagaacacc ttgccgctga ccgtggtcag gagttgggga
    15901 tcagatgaca gcgttttcga tgcatcattg gggtcaacca cctggttgat gtccaactgc
    15961 cgcgtcgtgt tggtaccatc cgttttccag agttcgcgac cagtcgtacc gtcatccgcg
    16021 ctgaacatga cgacggagcc ggagaggagg gcgaacccgg ccggagacga actacctgcg
    16081 agaggattga tatccttgag cagggccgta gcaacggtca gattgttcct gagggttgcc
    16141 ttccacagtt caacgccgtt ggtgccgtca tccgccgcga aataaagctt atcgtactgc
    16201 gcaaacatat tggacgggtt tgatccaggg gtgcccggat cgatgtccag ttcgagcgtc
    16261 atgacaggat ataagggctg gaggacgcga tagagctcct ggccggcgac acccgaaccg
    16321 tcgtccgcag acacgaaaag gcgcgtgatg acatcgaagg aaatcgcctt catatcgcca
    16381 atggtcgagc cgtcggggcc gggtcgaagg tcgagaatca tccgagttcc ggtgccgacg
    16441 ggcaggaggc tccgaagaca actggagccg ttatccccgc gcgtgccgtc cttaaacagc
    16501 aacgcgggtg aacatgcacc atcgacgctc cagtagggta cagaaccttc gggtattgca
    16561 aagaaggtat tggaggtacc ttcgaaaggc tgctgttcgt tcctgaactg ggccagcaag
    16621 ccgtttgtgg gcaccagaag ggccgtcgcc gcgaacacgc ctgcgagcct ggaacagatg
    16681 ttgggtgtac tggacatgac tctttcctcc ttcgaagtgt gggtcgtgaa gacccagtag
    16741 acagtcaacc agaagggccg tgtcccggtg tgctgttttg gacgcgaggc cattggccgt
    16801 tttttcgcac ggcacccgga agcgaacagc ccagaaaaca tctagctatt gttaccttca
    16861 cccaaatgta tacatgattg aggctgttct gtcaacagga gtcgaacgtg tcctgtgcgg
    16921 gggcagggca atcgcatcta aaccgtgatc ctcccatcac tttttttgca tgttaggtgt
    16981 atgtaaggat tgcgccctcg ctgaattgtg gataacttcg gccccattgg tcacgaggcc
    17041 tcgtgttgtt tagaggttta tccttttttc aggagctgca acatggacgt tgaagccccg
    17101 cgtggcctgc tgcgcttttt tgatgagttg gaggatcccc ggatggagcg gacgaaattg
    17161 cattcgctcg aggacatttt gttcatcgcc ctttgtgcgg tcatctgcgg ggcggacagt
    17221 tggaccgagg tggaggtgtt cggctgggcc aaacgggaat ggctgagtca gtacctgacg
    17281 ttgcccaacg gcattccttc ccatgatacc ttcgggcggg tgtttagcag gctggacccc
    17341 caagtattgg agcgatgttt ctcgaagtgg atggcggccc tggccgaggc cagcgccggc
    17401 cgattggtgg ccatcgacgg caagaccctg cgacgcagtt ttgacaaggc cacgaatcga
    17461 gcggcgatcc atatggtcag tgcctggtgc gagacgaacc gtctggttct ggggcaactg
    17521 gcgaccgcag aaaagagcaa tgaaatcaag gctatcccac agcttctgaa gatgctggat
    17581 atccgtgatg cggtggtgac gatcgacgcc atgggctgcc agaaggccat cgcccaggag
    17641 atcgtccagc aaggcgggca ctacctgtta caggtcaagg acaaccagcc ggggctgcac
    17701 gatgtcgtca aagcgacctt cgacgagttg accggccgag gaatcgccgg ggtccagtac
    17761 gatttccatg agcagaccga tgccggccac ggccggatcg agacccgtcg tttgtgggtc
    17821 accgactgga ccgattggta tgcggaccga aaagagtggg ccaacctgac cagcttcgtc
    17881 tgcatggaaa gcgtacgcac ggtgaacgga tccacttcct gcgaacgcca ctattacatc
    17941 agcgatctgg ccggccaaag tgcccagacc atgctcggct acgtccgcgg ccactggggt
    18001 atcgagaaca agctgcactg gtcactggat atgaccttcc gggaggacac cctccgtaac
    18061 cggatcggcc actccgccga gaacctctcg cgaatccgcc gactgagcct taacctgctc
    18121 tgtcgagaga aaacctgcaa ggccagcctg aaaagcaaac gcctgcgagc cggcctacgg
    18181 gaggactact tggtccgggt cctgtgccag gggatttaga tgcgattgcc ctgtgtgcgg
    18241 ggggccggtg aaggcgagtt ctgggccgtt ggattgccag cctgggaacc tgggggcaag
    18301 tcgccaaaga tggtttgggc agctttgccg aggtgacctg ggaggggcgg atgactgaag
    18361 attcgcctag gtgtttgata agtctttatt ttacagaaac ttaaggtcat ttttgggttc
    18421 gtttggcgcg gaggagctgt cagcggccag cgatcggcca tcagccataa aagcagactc
    18481 gtccgtgggc gtggaagctg ccggccttgg gccgcaggag gttggttttc acttttcaaa
    18541 gagcgacggc ggaggcggtg gacggtctgg ttccggtcgg cgcgcaggat ctccgccagt
    18601 tttgtcggga gggagaacac gaggtgcgct ccggaacggg cgggttgaaa gggagcggcg
    18661 gtagggcagg ggggagcggg ggccggccag ctttcaaccc gcaggcgtgg atcttacccg
    18721 actgcgggga cctgcgacct gtcctgcttt ctatggcggg tccggcttgt cctggctact
    18781 ccattttcca ctcggtcagt tcatcttcgc tttcgatgaa acgtccggtc gggatttcgt
    18841 gcgaccagtc ggtgaggccg aatttgatgt ggccagcggt gaaggtggcc agaaggtctc
    18901 ggtcacccct ggtgcgggcg atctcgatgc cggtggcgcc gggccagaac tcacggcgtt
    18961 tggcgaagtc gaacttgagg ttggtgtcgt tctcgataat ccagttgacc gccttctcat
    19021 tgcggatttt ggccttgacc gtcaggaacc acctgccttt cctgaagaag aggttcgatg
    19081 attccagact gccctgcggg tggccgccct ccaggcgagg ctcgtatccg gtggcggggc
    19141 cgacgcaaat gggcccgtgg tcctgccatc gcttgaagtc cgtagtggag accatcgcga
    19201 tgcatgactc cccctggcgg gtattggcgg tgaagatcat ccagtagcgc ccgtcatgct
    19261 cgcagatgct cgggtcccgg cagcttgaga tcatgtcctc ccgccagaag gcccattcct
    19321 tgcccttgca cggcgagata gggttgctgt cccaacgccg ccactcgaag agatctttgc
    19381 tggaggccag gccgatgttc tgcgagatgt gtcggttcag gccggtatag gccatgacga
    19441 actcatcgcc gcggcggatc acgcagggcg cccagacgtg ggcctcctcc caggttcccg
    19501 gccggatcag catgaccgga tcgtgaacct cccagttgac gaagtcggac gtcgaggcgt
    19561 gaccgaagaa gatctcatgg cctggataga agggcgtacc ctcctgcagc cgccgctcga
    19621 tgtagaagaa gtgcagtcgg cctttgaagt ctgcaacgcc gaagtcggcg acgttcccac
    19681 cgaccggccg aaagccggtt ttcacgatgt ccaccttgcg gtgacggtag ttcctgtccc
    19741 gcatggcaaa gagcgttttt tcaaactcga tgttgttgcc gccgaccgtt tcctggccga
    19801 gggttgtccg ggccgcagcc agcgtggccg ccccggcacc ggcgaggcga agaagtctgc
    19861 gtcggctgaa cgctacgtta actgttcttg tcgtttcttg cattgatggt ctccggtggg
    19921 gagggtttgg tggtcgtggt cgagatcggc aggtctccgc cgcctttggc ggctccgacc
    19981 gtgccctacc ttgctgttct tgtcgtttct tgcattgacg gtctccggtg gagggggttt
    20041 ggtgatcgtg gtcgagatcg gcaggtctcc gccgcctttg gcggctccga ccatgcccta
//
LOCUS       DAOTPW010000093        20055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2755_length_20055_cov_4.616350, whole genome shotgun sequence.
ACCESSION   DAOTPW010000093 DAOTPW010000000
VERSION     DAOTPW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20055
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2755_length_20055_cov_4.616350"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="P5316_10135"
     CDS             <1..611
                     /locus_tag="P5316_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRR85359.1"
                     /translation="GFGASGLLVPAMVWLIDSYGWRSAMILQGAGMWVLGIPLALVIR
                     DRPEDIPPLVQFFLRQYASYYPRPIEGVDERVYEVLSRTIGSGNVRELENAVRQILAF
                     KVAGTRIELSDIPPSLLKKQATPDPDVEMVNVLSSAVDAMLRGGRLTLAEMVEQFEAM
                     ILNEAMSRSTVTHCELAQRLGLSRRTFYHKLRKYDLPRPKKP"
     gene            complement(627..1199)
                     /locus_tag="P5316_10140"
     CDS             complement(627..1199)
                     /locus_tag="P5316_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR85360.1"
                     /translation="MALTPSTMLPLGTRAPDFQLPDTSGKTVCLSDFSEAPALLVIFM
                     CNHCPYVKHVRAELARMTAEFKDQGVAVVGINSNDVNSYPEDSPEKMREEAVAAGYAF
                     PYLYDSSQQVAKAYRAACTPDFFLFDGQRRLVYRGQMDDSRPGNGKPVTGADLRAAVS
                     AVLAGEPVHSDQKPSIGCNIKWKPGNEPTY"
     gene            1370..2977
                     /locus_tag="P5316_10145"
     CDS             1370..2977
                     /locus_tag="P5316_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85361.1"
                     /translation="MMTRGKTCLLKGFLLVLAATARSPAALADTVTKPIDISDRRQVF
                     IDGRFMTRAKGLELTVHQPRKTGEMTIKPDHEWETGGVGPYSSVMKVGDTYHMWYHVM
                     DTVQWHTGEKNGCICYARSKDGIHWEKPELGLIEYKGNRRNNIVVGHGAAGAQIGQDG
                     VMVFLDPNAPDDERFRMLGRFDVPVEAGQAIHIFSSGDGIHWKKTHESVCTARPQEQG
                     HHLDSQNVIFWDDRINKYVCYVRKNRSDPGSQGRCIARGESDHLGGFPAVQDLPIVFG
                     GDRDDLHHGDTPVVDFYMSAAIKYPWAQDAYYMFPTAYYHYINGLAEFPKEVPTNAGP
                     LDTRFAASRDGINWQRFDRRSFVPLGMKGEFDWASTRVIWGLVPDVSGREMYLYYRAS
                     DWLHGWDRDERNKRLLAEAGLNASQNIAVLSRLVSRRDGFVSVRGAYTGGEFTTPPLV
                     FQGSKLSLNVDTSAAGIVQVEILDADGRPIKGFALDDCDLIHTCNEVDRAVTWKRNSD
                     VSSLAGKPIRLHMTIRDADLYAFQFQQ"
     gene            complement(3135..4565)
                     /locus_tag="P5316_10150"
     CDS             complement(3135..4565)
                     /locus_tag="P5316_10150"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRR85362.1"
                     /translation="MLSTLIRLCSQAAARRNGIILTLAMASTLPAFSLAARAQGVAKS
                     PADVRAHMLREAHRSKPDYVVYVPQHWDSSRHDSHNEHFLVFEGPDGSLMAVWTQALE
                     QPGPKNRIVFSRSDDDGKTWASPSHVAGPRTPDDPTHMASWAFPMVSRRGRIYVVYNQ
                     HQGISGWIPMHTGTMDAVYSDDNGRSWSKPQTIPMPKSPYDDPAGKTPAEWIVWQIPM
                     RDMNGGFLVGYTHWLNPARATLKKVEGWTQIESVCEFMRFENVDANPEPKDLKVSYSG
                     WGDKALRVPHFMHPLLSIAQEPSIVRLPDERLFCVMRTNSGYIWYSVSADDGRTWCNP
                     RPLLRKDHGQPILQPVSCCPIYQLADGRYVLLHHNNRGNIEGRSERTSEPRRPAFLAL
                     GEFRPTADQPIWFSESKQFMDNDNVGPNGLSKEVDPKVNTAIGIYSSFTTRGGNNVLW
                     HPDRKCFLLGKKVTDEFLADMTVPKE"
     gene            complement(4665..6251)
                     /locus_tag="P5316_10155"
     CDS             complement(4665..6251)
                     /locus_tag="P5316_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR85363.1"
                     /translation="MLLRTTLIGLTAVVAMSTTSSAQAINEEFRSIFDGRTLNGWDGD
                     PVYWRIEDGAITGQTTPDKPLKHNTFLIWRDGELDDFELVLEYRIFGGNSGIQYRSWE
                     DPDKWGKWVAAGEQADIDATNKYTGILYSERSRGILAQRGQKVVIGEDHKARVVGTIG
                     SAEELAAKIRNQDWNSYRVVARGNRYVHEINGQVMIEAVDEDRQASRRSGILALQLHA
                     GDPMKVQFRDIRLKRLPMEGKKKIVFVAGTASHGYGSHEHNAGCLLLARLLNENVPAV
                     HAVVYRNGRPTDPTAFDNANAIVFFADGAGGNMINNRLAEVGAAVDRGAGLAVLHYAL
                     DVPKGPVGDHMLKWIGGYFEQHWSVNPHWSARFAELPNHPITRGVRPFEIEDEWYYHM
                     RFRENMENVTPILSAVPPERTREGPDGAYSGNPHVRARKGMLEVVAWAFERPDGGRGF
                     GFTGGHFHWNWAHDDYRKLMLNALTWVAGAEVPPKGVPSKTPTFEELDANQDEPRPAD
                     FKVETIRAKLSQWSAATRPS"
     gene            6680..6880
                     /gene="thiS"
                     /locus_tag="P5316_10160"
     CDS             6680..6880
                     /gene="thiS"
                     /locus_tag="P5316_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRR85364.1"
                     /translation="MRVQINGKDEDIDPGTTVAALVGRYNLQPKHVAVEINCDLVPRK
                     VFDTTRLKEGDRIEIVTLVGGG"
     gene            6881..7717
                     /locus_tag="P5316_10165"
     CDS             6881..7717
                     /locus_tag="P5316_10165"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRR85365.1"
                     /translation="MTDAGIKLGPFQFSSRLFVGTGKYSNYEVMNQCLAASGCQVVTV
                     AVRRDVLRPEGGKREQSLLDALDPTRYTILPNTAGCFSAEDAVRAARLSRELLEGLDN
                     PGAQWVKLEVLADKRTLLPDPIGSLQATEKLVKEGFAVLVYTSDDPIMAKRLKEAGAT
                     SVMPAGSPIGSGQGVLNPNNIRIIIEYLKENDPDYPVIVDAGVGTASDVTVAMELGSD
                     GVLLNTGIAGAADPVRMAHAMRHAVEAGRLAFLAGRIPKKLYATASSPTEGVIAPAAR
                     TR"
     gene            7729..8181
                     /locus_tag="P5316_10170"
     CDS             7729..8181
                     /locus_tag="P5316_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85366.1"
                     /translation="MQRRTRNRICIWLIVGGLANFLVYTVVYAYLGGDARNGAKEKIT
                     DPDGRVEEMFYIRGHFLHGAEVGRPTAVSRAVWIYSYLHSISIWPTQGVMMICMLILA
                     QPHIIATMQESNWIRGPTFVAVAITLVAVVYSAMSIWFAIGFVRDLTA"
     gene            8201..10147
                     /locus_tag="P5316_10175"
     CDS             8201..10147
                     /locus_tag="P5316_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRR85367.1"
                     /translation="MSTVSEVLGIDGLIARRLAGYEHRPQQLEMAAAVERAFGRSRHL
                     LAEAGTGVGKSFAYLVPAIQRVTQANERVVISTHTIALQEQLIQKDVPFLAAVGPEEF
                     TAVLVKGRNNYLCIRRLRQTSERQTSLFADSLRDELWRIEDWAYKTTEGSLAELDPVP
                     DPRVWELVRSEHGNCLGRPCPHYNACFYQRARRRAAHAQILVVNHALLMTHLALGRRK
                     VSILPPYQLVVIDEAHTLEAVAADHFGQSVSQGQVRFLLNRLHSERTGRGFLSVFGAR
                     ESLRAVHRARLAAHRFWEDLWDWKQLHGRPNGRVTDRIPVDDPLTESLRALRISLLAL
                     QDRITRQEDLVELASLAERTGALAAELESLLGEARPNTVRWLEGRGPQAGDITLSEAP
                     ISVAEHLREHLFGKNRSVILTSATLAVGRKNGFAYIRSRLGLDEADEIQLGSPFDYQR
                     QAELHIEAGMPEVDDADAFLPAAADAVARHIESTDGHAFVLFTSYEMMSDLAARLRSR
                     LQDMGLRLMVQGEGMPRSLMLERFRNEPGWVLFGTDSFWQGVDVPGEALTNVIIVKLP
                     FMVPDRPVVEARIEHIRACGGNPFMDYQLPEAVLKFKQGFGRLIRTRSDHGKVVVLDR
                     RIKTKRYGKRFLDAIPQCRVIVHA"
     gene            10171..11352
                     /locus_tag="P5316_10180"
     CDS             10171..11352
                     /locus_tag="P5316_10180"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRR85368.1"
                     /translation="MIRNGSSCCYYAISFFACALACGCAATKPRPSYGSKPIFEERVL
                     FQSGEGGYHTYRIPSLIVTPKGTLLAFCEGRKNSAADYGDIDILLRRSSDNGQNWNPV
                     QLVVDHGPNTIGNPCPVVDRRTGRIRLLLTGNLGDDSEDKILKRVARQTRTVWACCSI
                     DEGQTWTMTVDITADTKLPEWTWYATGPGCGIQLENGRMVIPCDHNVAEPEDIRRSHV
                     IYSDDHGETWKIGGVVGNHVNECQVVQLVDGSLMLNMRNYARGEDRQNHRAVATSGDG
                     GLSWSPIWWAKELVEPVCQASFIRFTKRPNFSKNRLLFANPAGIKREKMTIRVSYDEG
                     LSWPVAKEMYSGPSAYSALAVLPDMTIGCLYERGEKSPYETITFARLSLEWITDGLEN
                     P"
     gene            complement(11554..12795)
                     /locus_tag="P5316_10185"
     CDS             complement(11554..12795)
                     /locus_tag="P5316_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRR85369.1"
                     /translation="MRHAIVRRAIVATVLAAWHSCASAQDRAVWHEYPDAFKQRLGQR
                     IEPATRPGEPGPVLMLDSSTVDAPKSPADFKQAWHQPPVSQGLTGQCWDFSATSFLES
                     EVHRQTGRELRLSVAHTYYWEFVEKARESVRTRGQSVFNRGSEPDAVLRIWRSYGVVP
                     AEAYCGLAAGHEILQDAAAYHEIAAHLAKVKLEGRWDDSLVVAAVRRILDKHLGRPPE
                     TVTTDGKVMTPREYLRQVVRLDLDDYVSVMSTMQVPYDQWSEFKVVDNWWHSRRYYNV
                     RLDDFVRVIRHAARVGRTVCLGIDNTEPGYCFREGFAVVPTFDIPPVLIDDGARQMRL
                     FNASTSDDHDVHLVGWLEKEGQMWYLIKDSGTKAWNNSHNGYMFYREDYVRLKVLCAL
                     LSRDIIEQALGRGIGMPPLDR"
     gene            13230..15428
                     /locus_tag="P5316_10190"
     CDS             13230..15428
                     /locus_tag="P5316_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR85370.1"
                     /translation="MSDAVTQSVSFLGRFTVLKGAARELWITFAIKLLGIIAYSVMNT
                     TFVLWLSYDLGYSDEHAGYWVGAWSALMTLLTVLVGSLTDAIGLRKAFLLGVIVCVGA
                     RAVMTFTAIPWVALGAGLLPLALGEALGTPVLVAAIRRYSTTAQRSISFSIFYAMMNV
                     GFLIASYIFDYVREGLGEHGRFTLPLVGIELTSYRTLFLISLIVEILMLPIVWLIREG
                     VEATDEGVKITPGERKGPHENVLSALILMIRDTLRESARIFAGLWRQSGFYKFLAFLA
                     LAAFVRLIFIQMYYTYPKFGIRELGEGAAVGRLWAINPFLIIFLVPLVGAISQRISAY
                     RMVSIGSAIAAASVFIMAMPPAWFQPLADGRLGHLIANTWLGGGSRFTPDDFLDLPSL
                     TRRFASASDPASLAVSESFSEATRALLHRRVAVADRISRSTQHPSSALLIAADIADPD
                     SFVARLQKDPEPATRPVSDYLWGRFPAAQRQKMTDANLPPTKRIAVLLEELNRVLKDE
                     SFCEKHRDVFRAIQLSEATRALLKLDPRGSEDVLSNLSVLQNRLLLEDAYPSHIAASD
                     HPLRAALAHDLSRMIRKSTVHIRFADGRQLAATRPVLEDVFSVEIARNRVGVPGSVSP
                     WYVMIFLFIVLLSLGESIYSPRLYEYAACIAPKGQEASYMALSYLPFFLAKLFVATFS
                     GVLLARYCPEAGDRHSGTLWLIIALITTIAPVGLVTLRRWIRVPEAGRGD"
     gene            15501..16637
                     /locus_tag="P5316_10195"
     CDS             15501..16637
                     /locus_tag="P5316_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85371.1"
                     /translation="MRFSRLCALPVMLAWCGRTTVPAAAAATSIPPTDKVKIGVIGLV
                     HGHVYGFLEDAVRRTDVEIVGLAEPREELLRRYGERHKFPESIRFKDLDQMLDTARPQ
                     AAAVFTDTLEHRRVVQACAPRGIHVMMEKPLATTMEHARAIEKAAREGRIHVVVNYET
                     TWYPNTQAAYQLVSKQGRIGPIRKIVAHDGHQGPKEIGMPREFMEWLTDPVRNGGGAL
                     FDFGCYGANLATYLMGEQRPLSVTAVTQKLKSDPVYERVDDEATIVLTYPTAQVIIQA
                     SWNWPFNRKDIEIYGEQGQYLTVERDMYRMRTLHAAETLNKAEPIAAPMQDSISYLAA
                     VVRGEVLPSGPSSLKVNMIATEILDAARRSAEHGVTIPLPDVMP"
     gene            complement(16945..18084)
                     /locus_tag="P5316_10200"
     CDS             complement(16945..18084)
                     /locus_tag="P5316_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85372.1"
                     /translation="MAECNFTVSGFARTEDLPVCERLGLAAIMAPPDDEVPWKRPWRA
                     LSDEEIDQRLRTMVKLAGNSSAVLGYFIVDEPGTREFGALAKAVAAVKRYAPGKLAYI
                     NLFPGYATIGAPDKSQLGAASFTEYLERYAAEVKPQFISYDNYMIQYSDDLLDAGRAK
                     NYFADLLEVRRVALKHDLPFWNIVSSNQIRKNTTIPSPANMMLQAYTTLAAGGRGLSW
                     YTYYGGSGPRPAYAYAPIDHTGNKTETWGYLQLVNHQVKTLGRIMNRLKSTGVFFDGP
                     GMLDSLPPLPGRIIKEVHSTISPRGFAKTSLPVMIGEFTDDSGSDYVMIVNLSIERSG
                     NLRITFAKAYGSKEIISAQDGHVVALDEENGLWLTAGQGALIRLR"
     gene            18533..19504
                     /locus_tag="P5316_10205"
     CDS             18533..19504
                     /locus_tag="P5316_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85373.1"
                     /translation="MSCHTIAFSRCLMILSLAGLLTGCHPTPVGPTRPPATSPAPVPK
                     RYSYTPTPYPLDERGFSPVDKALAAAYACEEMIADDAEGSLNPYVLKVISAYPIDGSY
                     PYHCSWKPREYDIYNGVTQDMWYKGMIVAKAYPDGSRCSYCCGLTFEIFIRAMKLRNI
                     QKGLDPDDFNGMTFHDLFNMLQLWYIEGKDDSEQRAIVSYGLGRAITDFEEVRPGDFL
                     SYSTTPPGGHSVIFINWLRDESNKIIGLRYFSSNLSGSKGPGYGEGRFSDCNNGRGLL
                     RNSLRIARVGAIKDYKPFDRLKIPRRNAYAPNQPDRIIYLPATRPAQ"
     gene            19581..>20055
                     /locus_tag="P5316_10210"
     CDS             19581..>20055
                     /locus_tag="P5316_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85374.1"
                     /translation="MRYLFVVAAVLMVLPGRASGQTPDNLCRNPGFELLNRAGDNFPM
                     NWTAVHNPAGQGTVIIDKTTHAGSIAVRMSATPDCYVGINSDPIPATKGVVTFWYQIV
                     RSDSGGENLRLFVIAVAGDAAEVVRAGYELPVEHVGDGRWHQGRAEFDFSSHAGVN"
BASE COUNT         3840 a         6335 c         5907 g         3973 t
ORIGIN      
        1 cggggttcgg cgccagcggc ctgctggtgc cggccatggt ctggctcatc gacagctacg
       61 ggtggcgttc ggccatgatc ctgcagggcg cgggcatgtg ggtcctgggg atccccctcg
      121 ccctggtcat ccgagaccga cccgaggaca tcccaccgct ggtgcagttc ttcctccgcc
      181 agtacgcctc ctattatcct cgtcccatcg aaggcgtcga cgagcgggta tacgaagtcc
      241 tttcacgcac gatcggctcc ggaaacgttc gcgagctgga aaacgcggtt cggcagatcc
      301 tggcattcaa ggtggccggt acgcgaatcg aactgagcga cattccgccg agcctgctga
      361 aaaagcaggc cacgcccgat cccgatgtcg aaatggtcaa tgtgctcagc tccgcggtag
      421 acgccatgct ccgtggcggc cggctcaccc tggccgagat ggtcgagcag ttcgaggcaa
      481 tgattctgaa cgaggccatg tcgcgatcca cggtgaccca ttgcgaactg gcacaacggc
      541 tgggcctcag ccgacgaacc ttctaccaca agctccgcaa gtatgatctc ccgcggccca
      601 aaaagccctg aaacggccac atagcctcag tacgtcggct cgtttcccgg cttccacttg
      661 atattgcagc cgatgcttgg cttctggtcg gaatggaccg gttctcctgc caggacggcg
      721 ctgaccgccg cccgaagatc ggcaccggtt acgggcttgc cgttgccggg tcgactgtcg
      781 tccatctgac cacggtagac cagtctgcgc tggccgtcga ataagaaaaa gtccggcgtg
      841 cacgcggccc gatatgcctt ggccacctgc tgactggaat cgtaaaggta gggaaacgcg
      901 tagccggccg ccacggcttc ctctcgcatc ttctcgggac tgtcctcggg ataactgttg
      961 acgtcgttcg agttgatccc cacaacggcg acgccttggt ccttgaactc agcggtcatt
     1021 cgcgccagtt cagcccgaac atgcttcaca tagggacagt ggttacacat gaagatcacc
     1081 aacagagccg gcgcttcgga aaaatccgaa aggcataccg tcttccctga agtgtccggc
     1141 agttgaaaat ccggagcgcg agtccccagg ggaagcattg tcgatggcgt caatgccata
     1201 ccgtcggtcc tccaatgtgg ttggtacggt gcttttcatg tcattgtaga cctccccccg
     1261 gcctttgacg aatcccgatc ggcctgtgtg aacttgcggg ctgtccaagt gccccggtat
     1321 gctctgcaca ggcccggcaa atgggtgtgc gaaaggaaag gtgcgactaa tgatgacccg
     1381 cggaaaaacc tgcttgctca agggtttcct gcttgttctg gctgccactg cccgctcacc
     1441 ggcagccttg gcggacacgg tcaccaagcc cattgatatc agcgaccgca gacaggtgtt
     1501 catcgacggc cggttcatga cccgggcgaa aggcctcgag ctgaccgttc accagccccg
     1561 aaagaccggc gaaatgacta tcaagcccga ccacgaatgg gagacaggcg gcgttggtcc
     1621 ttatagcagc gtcatgaagg tcggcgacac ataccacatg tggtatcacg tcatggacac
     1681 cgtgcagtgg cacaccggcg agaagaatgg ctgcatctgc tacgcccgct ccaaggatgg
     1741 catccattgg gagaagccgg aactgggctt gatcgagtac aagggcaacc gcagaaacaa
     1801 catcgttgtc ggccacggcg ccgccggcgc gcaaatcggc caggacggtg tcatggtctt
     1861 ccttgatccc aacgcccccg acgatgagcg ttttcgcatg ctcggcaggt tcgacgtgcc
     1921 cgtcgaggcg ggccaggcga ttcacatctt ctcctcaggc gatggcatcc attggaagaa
     1981 gacccatgaa tcggtctgca ccgcccgccc gcaagagcaa gggcatcacc tcgactccca
     2041 gaatgtcatc ttctgggacg accgcatcaa caagtacgtt tgctacgtcc gcaagaaccg
     2101 cagcgacccc ggctcccagg gccgctgcat tgctcgcggc gaatcagatc atctgggcgg
     2161 ctttccggcc gtgcaggacc tccccatcgt cttcggcggc gatcgcgacg accttcacca
     2221 cggcgacacg ccggtggttg acttctatat gagtgcggcc attaaatacc cgtgggccca
     2281 agacgcctac tacatgttcc cgaccgccta ctatcactac atcaacggct tggccgagtt
     2341 tcccaaggaa gtcccgacca acgccgggcc gctcgacacg cgatttgcgg ccagccgcga
     2401 cggcatcaac tggcagcgat ttgaccgccg gtcattcgtc ccgctgggca tgaaagggga
     2461 attcgactgg gcctccacgc gggtcatctg gggcctcgtg ccggacgtgt cgggacgcga
     2521 gatgtacctg tactaccggg ccagcgactg gctccacggt tgggaccgcg acgagcgtaa
     2581 caaacgcctc ctggccgaag ccggcctgaa cgccagccag aacatcgcag tgctcagccg
     2641 gctggtctca cgccgcgacg gctttgtctc cgtgcgagga gcctacaccg gcggcgagtt
     2701 caccaccccg ccccttgtgt tccagggaag caagctcagc ctgaatgtgg acacctccgc
     2761 ggccgggatc gtccaggtcg agatcctgga cgccgacggc cgccccatca agggatttgc
     2821 gcttgacgat tgcgacctga tccacacctg caatgaggtc gaccgggcgg tcacctggaa
     2881 acgcaacagc gacgtgagtt cgctggcagg caagccgatc cgcctgcata tgaccatccg
     2941 cgacgcggac ctgtatgcct ttcagtttca gcagtagctg tcgccgatga gctgtcagcg
     3001 atcagcgatc agcccaagcg agccgccgcc tttgacctgt ggccccggat tcccatccgg
     3061 ggccgctcag tccatttctt cgatgccgcg caccccgaga ccgcatcgac aaccttggtg
     3121 acaattgtgc gggtttactc cttcggcacc gtcatgtccg ccagaaactc atccgtgacc
     3181 ttcttgccca gcagaaagca cttgcggtca gggtgccaca aaacgttgtt gccgccgcga
     3241 gtggtgaagc tgctgtaaat gccgatggct gtgttgacct tcggatcgac ctccttgctc
     3301 aggccgttcg gcccgacgtt gtcgttgtcc ataaactgct tggattcgct gaaccagatg
     3361 ggctgatcgg ccgtcgggcg gaactcgccc agcgccagga acgccggtcg tcggggctcg
     3421 ctggttcgct cggatctccc ttcaatattg cctcgattgt tatgatgcag cagaacataa
     3481 cgaccgtcag ccaactggta aatggggcag cacgacaccg gctgaagaat gggctggccg
     3541 tggtccttgc gcagcaacgg acgcggattg caccacgttc tcccatcgtc ggccgacacg
     3601 ctgtaccaga tgtagccgga attggttcgc atcacgcaga ataaccgttc atccggcagg
     3661 cgaacgatac tcggctcctg ggcgatgctg agcagcgggt gcatgaagtg ggggacgcgc
     3721 agggccttgt cgccccaacc tgaatagctg accttcaaat ccttgggttc cgggtttgca
     3781 tctacgttct caaaacgcat gaactcgcac accgactcga tctgcgtcca gccctcgact
     3841 ttctttagtg tggcccgggc cgggttgagc cagtgagtgt atccgacaag aaagccgccg
     3901 ttcatgtccc gcatcggtat ctgccagacg atccactcgg ccggcgtctt gccggcaggg
     3961 tcgtcatacg gactcttggg catcggaatg gtctgcggct ttgaccaact gcggccgttg
     4021 tcgtcgctgt acaccgcgtc catcgttccg gtgtgcatgg gaatccagcc gctgatgccc
     4081 tggtgctggt tgtagaccac atagatacgt ccacgcctgg ataccatcgg gaatgcccaa
     4141 ctggccatgt gcgtcgggtc gtcaggcgtc cgcggcccgg cgacgtgcga aggcgatgcc
     4201 caggtcttgc cgtcatcatc ggagcgcgaa aaaacgatgc ggttcttcgg gccgggctgt
     4261 tccagagcct gcgtccacac ggccatcagc gaaccgtccg gcccctcaaa caccaggaag
     4321 tgctcgttgt gcgaatcgtg cctcgaactg tcccagtgct gcgggacata gacgacgtaa
     4381 tccggcttgc tgcgatgggc ctcgcggagc atgtgggccc ggacgtcggc cgggctcttc
     4441 gcaacgccct gagcccgtgc tgccaatgag aacgccggca gggtactggc catcgccaat
     4501 gtcaggatga ttccgttgcg acgagcagca gcttgtgagc agaggcggat cagtgtcgac
     4561 agcatggttt gttctccaga gatagccaca gatagaccct tcaattcatc aggtcaccgg
     4621 tggcgtcgat tgtcagcttc aatggtgttc gagtgtctcg cttgtcatga aggacgagtc
     4681 gccgcgctcc actggctgag cttggcccga atcgtctcaa ccttgaaatc cgccgggcga
     4741 ggctcatcct ggttggcatc caactcctcg aacgtcggcg ttttcgatgg cactcccttg
     4801 ggcggcacct cggcgccggc cacccacgtc agggcgttga gcatcagctt gcgataatcg
     4861 tcatgcgccc agttccagtg aaagtgccca cccgtaaacc cgaatccgcg cccaccatcg
     4921 ggtcgctcga aagcccatgc caccacctcc aacatgccct ttctcgcccg aacgtgcgga
     4981 ttgccgctat aggcgccgtc cggcccttct cgtgtccgct caggcggaac tgcgctgaga
     5041 atcggcgtga cgttctccat gttctcgcgg aaccgcatgt ggtagtacca ctcgtcctca
     5101 atctcgaacg gcctgacccc ccgcgtgatc gggtgattcg gcaactccgc gaaccgcgcg
     5161 gaccagtgcg ggttgaccga ccagtgctgc tcgaagtagc cgccgatcca tttgagcatg
     5221 tggtcgccga ccggtccctt gggcacgtcg agtgcgtagt gcagaacggc caagccggcg
     5281 cctcggtcaa ccgcggcccc gacctcggca aggcggttgt tgatcatatt gccgccggcc
     5341 ccgtcggcga agaagacgat cgcgttggca ttgtcgaacg ccgtcgggtc ggttggccgg
     5401 ccattgcggt acaccaccgc atggacggca ggcacattct cgttcagcag tctggctagc
     5461 agcaggcacc ccgcattgtg ctcgtgactt ccatagccgt gactggccgt gccggccaca
     5521 aatacgatct tcttcttgcc ctccatcggc agacgcttaa gccgaatgtc gcggaactgc
     5581 accttcatgg gatcgccggc gtgaagttgc agggccagga tgcccgaacg gcgcgaggcc
     5641 tgacggtctt catcgacggc ctcgatcatg acttgcccgt tgatctcgtg cacgtagcgg
     5701 ttgccgcggg cgaccacgcg gtagctgttc cagtcctgat tacggatctt ggcggccagt
     5761 tcctcggcac tgccgatcgt gcccacgacc ctggccttgt ggtcctcgcc aatgacaacc
     5821 ttctgacccc gctgggccag gatgccccga gaccgctcgc tgtacaagat gccggtgtac
     5881 ttgtttgtgg cgtcaatgtc ggcctgctcg ccggccgcga cccacttgcc ccacttgtcg
     5941 ggatcctccc aactgcgata ctgaatgcca ctgttcccgc cgaagatgcg atactcaagc
     6001 accagctcga aatcatcaag ctcgccatct cgccagatga ggaaagtgtt gtgcttgagc
     6061 ggcttgtccg gcgtggtctg accggtaatg gctccatcct cgatgcgcca atagaccggg
     6121 tcgccgtccc agccgttgag cgttctacca tcgaaaatgg acctgaactc ctcgttgatg
     6181 gcttgggcgc tcgaagtcgt tgacatggca accacagcgg taagaccgat gagagtcgtt
     6241 ctcaacagca tgaccattcc tcccacgcag atcgagcccg gctggctgcc gggtcggccg
     6301 gtccccacgc aggttcaggc ttgggtcgat agtatagctt ctcgtcctga atctgggcag
     6361 cccaccgccg ccgcgccggc cacttcccca agcttgcatg caagacgccg ccgacgacct
     6421 ttccggctgg cagcttccac ctgctcgaac ggtctccccg gccgcttgcc cgcaatgcac
     6481 aaccgacgtg acgaaaacag cgccaacgac cgttatcccc tcctcaccgc aagccgtcga
     6541 tccgccggcc gcatgctggg atcggacatc tcgttgattc ctcctccggc cgtttctata
     6601 atcaggtcct ttgagatggt ctgaggtgtc caaccgaggc gcgcgagaca gatgttcgcc
     6661 tcaagaggcc gtgttcacga tgcgcgtgca gatcaacggc aaggatgagg atattgatcc
     6721 gggcacgacc gtggccgcgc tcgtggggcg atacaacctg cagccgaagc acgtggccgt
     6781 cgagatcaac tgcgatttgg tcccccgaaa ggtcttcgat acgacccgcc tgaaagaggg
     6841 cgaccgaatc gagatcgtaa cccttgtcgg aggtggttga atgaccgatg cgggcatcaa
     6901 gctcggtcct tttcagttct catcccgtct cttcgtgggc acgggaaagt actcgaacta
     6961 cgaggtgatg aaccaatgct tggcggcgtc aggctgccag gtggtgaccg tggccgtgcg
     7021 ccgcgatgtt ctgcggccgg agggcggcaa gcgggaacag agcctcttgg atgccctgga
     7081 tccgacccgt tacaccattc tgccgaacac ggccggctgc ttcagcgccg aggacgccgt
     7141 ccgggctgcc cggctcagcc gcgagcttct tgaagggctc gacaaccccg gagcacagtg
     7201 ggtcaagctt gaggtcctcg ccgacaagag gacgcttctg cccgacccga tcggcagtct
     7261 ccaggcaacc gagaaacttg tcaaagaggg ttttgccgtt ctggtctaca cctccgacga
     7321 cccgatcatg gccaagcgcc tcaaagaggc gggggccacg agcgttatgc cggccggctc
     7381 gcccatcggc agcggacagg gcgtcctcaa ccccaacaac atccggatca tcatcgagta
     7441 tctcaaggaa aatgatcccg actacccggt gatcgtcgac gccggcgtgg gaaccgcctc
     7501 ggacgtgacc gtcgcgatgg aactgggcag cgacggcgtg ctcctcaaca ccggcatcgc
     7561 cggagccgcc gaccccgtcc gcatggccca tgcgatgcga catgcggtcg aagcgggccg
     7621 actggccttc ttggctggcc gtataccgaa aaaactgtac gcgaccgctt caagcccgac
     7681 cgagggcgtc atcgccccgg ccgctcgaac gcgttgatgg agcctccggt gcagcgccga
     7741 acccgaaaca gaatctgtat ctggctgatc gtcggtggtc tggccaactt cctggtctac
     7801 accgtggtct acgcttatct ggggggggac gcccgaaacg gcgccaaaga gaaaatcacc
     7861 gaccccgatg gccgggtgga agagatgttt tacatccgcg gccacttcct tcatggcgcc
     7921 gaagtcggtc ggcccacggc cgtctcacgg gcggtctgga tctacagcta tcttcactcc
     7981 atctccatct ggccgacgca aggagtgatg atgatctgca tgctgattct cgcccagccg
     8041 cacatcatcg cgaccatgca ggaaagcaac tggatccggg gtccgacctt tgtggcggtt
     8101 gcgatcactc tcgtggcggt tgtctattcg gcaatgtcca tctggtttgc catcggcttc
     8161 gtcagggatc tgacggcgtg atgatcgagg cagtctgccc gtgtcgacag tcagtgaggt
     8221 tctcggaatc gatggactca tcgcccggcg gttggccggt tacgagcacc gtccccagca
     8281 attggagatg gccgcagcgg tggaacgggc gttcggacgg tcccgccacc ttctcgccga
     8341 ggccggcacc ggcgtgggca agagctttgc ctacctcgta ccggccattc agagggtcac
     8401 ccaggccaac gagcgggtcg ttatcagcac ccatacgatt gccctccagg aacagctcat
     8461 acagaaagac gtgcctttcc tcgcagccgt cggaccggaa gagttcactg ccgtcctggt
     8521 caaggggcgg aacaactact tgtgtattcg ccgattgaga cagactagcg aacgacagac
     8581 ctcgctgttt gccgattcgc tgcgtgacga gctctggcga atcgaggact gggcgtacaa
     8641 gacgaccgaa ggttcgctcg ccgagctgga ccccgtgccc gatccccgtg tctgggaact
     8701 ggttcggagc gagcacggca attgtctcgg acgcccctgc ccgcattaca acgcctgctt
     8761 ctatcagcgg gctcgccgcc gggcggccca tgcccagatt ctggtggtga atcacgcact
     8821 gctgatgacc catctggccc tcggtagacg aaaagtctcg atcctgccgc cgtatcaact
     8881 cgtggtgatc gacgaagccc ataccctgga ggcggtcgcc gcagaccatt tcggtcagtc
     8941 ggtctcccag ggccaggttc ggttcctgct gaaccgcctc cacagcgaac gcaccggtcg
     9001 tggctttctg tcggtgttcg gtgcccggga gagtcttcgg gccgttcacc gcgcccgcct
     9061 cgcggcgcat cgcttctggg aagacttgtg ggactggaag caactccacg gccgtcccaa
     9121 cggccgggtg accgatcgaa ttcccgtcga tgatcccctg acggaaagcc ttcgcgctct
     9181 tcggatctcg cttctcgcct tgcaggaccg aatcaccagg caggaggacc tcgtcgagtt
     9241 ggccagcctg gcggaaagaa ccggtgcgct ggccgccgaa ctcgagagct tgctcggcga
     9301 agcacgaccg aataccgttc gctggctgga gggtcggggc ccacaagcag gagacatcac
     9361 gctttccgaa gcacccatca gcgtcgccga acacttgcgc gagcacctct tcggaaaaaa
     9421 ccgaagcgtg atcctgacca gtgcgacact ggccgtgggg cggaagaacg gctttgccta
     9481 catccgctcg cgcctgggac tcgatgaggc cgatgagatc cagctcggat cgccgttcga
     9541 ctaccaaaga caggccgaac tccacatcga ggccggcatg cctgaggtgg atgacgccga
     9601 cgcgttcctc ccggccgcgg ccgacgcggt ggcaagacac atcgagtcga ccgacggcca
     9661 tgcttttgtg ctgttcacca gctatgagat gatgagcgat ctggccgccc ggttgcggag
     9721 tcgactgcag gatatgggcc tccggttgat ggtccagggc gagggcatgc cgcgctccct
     9781 gatgctggag cggtttcgca atgagcccgg ctgggtcctc ttcggtaccg acagcttctg
     9841 gcagggcgtc gatgtgcccg gtgaggctct caccaatgtg atcatcgtca agctgccgtt
     9901 catggtgccc gaccggcccg tggtcgaggc ccggatcgaa cacattcgcg cttgcggcgg
     9961 caaccccttc atggattatc agcttcccga ggcggtgctg aagttcaagc agggcttcgg
    10021 ccggctgatc cgcacgcgga gtgatcacgg caaggtggtc gtgctggacc gacgcatcaa
    10081 gaccaagcga tacggcaagc ggtttctgga cgcaataccg caatgccggg tgatcgtgca
    10141 cgcctgaagg ccggtcaacg aggtgtgacg atgatacgaa atgggtcgtc gtgctgctat
    10201 tacgcaataa gcttctttgc ctgcgctctg gcatgcggct gcgcggccac gaaaccacgg
    10261 ccttcctatg gtagcaagcc gatcttcgag gaacgggtcc tctttcagtc gggcgagggc
    10321 ggctatcaca cgtaccggat tccctcgctg attgtcacgc ccaagggtac gctcctggcc
    10381 ttctgcgaag gccgcaagaa cagtgctgct gactacggcg acatcgacat tctgctgcga
    10441 cgcagttccg acaacggcca aaactggaac cccgttcagc tcgttgtcga ccacggccca
    10501 aacactatcg gaaacccctg tccggtcgtg gaccgccgga cggggaggat ccgactgctg
    10561 ctcaccggca accttggtga tgacagcgaa gacaagattc tcaaacgcgt ggccaggcaa
    10621 acgcggaccg tttgggcctg ctgcagcatc gacgagggcc aaacgtggac gatgaccgtc
    10681 gatatcactg ccgacacgaa actgcccgag tggacctggt acgccacggg tccgggttgc
    10741 ggcattcagc ttgagaacgg ccggatggtg attccctgtg accacaacgt agccgagccc
    10801 gaggatatcc ggcgatcaca cgttatttac agcgacgatc atggggagac ctggaaaatc
    10861 ggcggggtag tcggaaacca cgtcaatgaa tgccaggtag tccaactggt cgacgggtcc
    10921 ttaatgctca acatgcgcaa ttacgccagg ggcgaagata ggcagaatca ccgggcggtg
    10981 gcgaccagcg gcgacggggg gctgtcgtgg tcgccgattt ggtgggccaa ggagctggtc
    11041 gagcccgtct gccaggccag cttcatccgg tttaccaaac gacccaattt cagcaagaac
    11101 cggctcctgt ttgccaaccc cgccggcatc aagagagaga agatgaccat tcgcgtcagt
    11161 tatgatgaag ggttgtcttg gccggtggca aaggaaatgt attccggccc gtcagcctat
    11221 tcggccctgg cggtactgcc cgacatgacc atcgggtgcc tgtacgaacg cggtgaaaag
    11281 agtccttacg agacgatcac ctttgcaagg ctctcgctgg aatggatcac cgacggattg
    11341 gaaaatccgt agggcatggt cgagcgcagc gagacctgcc gatttgccct gccgattttc
    11401 ccgtagggca tggtcgagcg gagcgagccc tgccgatctg cctgccgatt ttcccggtag
    11461 ggcatggtcg agcgcagcaa gccctgccga ttttcccgtg gggcatggtc gagcgcagcg
    11521 agacctgccg aattgcctgc gggcggcaac gtatcagcga tccaggggcg gcattccgat
    11581 cccccgtccc agggcttgct cgatgatgtc ccgagacagc agggcgcaga ggactttcaa
    11641 gcggacgtag tcctcgcgat agaacatgta cccattgtgg gagttgttcc aggccttggt
    11701 ccccgaatcc ttgatcaggt accacatctg cccctccttc tccaaccatc ccaccagatg
    11761 gacatcgtga tcgtcgctcg tggatgcgtt gaagagccgc atctggcgcg cgccgtcgtc
    11821 aatcagaacc ggcggaatgt caaacgtcgg caccaccgca aagccttcac gaaagcagta
    11881 gcctggctcc gtgttgtcga tacccagaca gacggttcga ccgacgcggg ccgcgtgtcg
    11941 gatcaccctt acaaagtcgt cgaggcgaac gttatagtac cgacggctgt gccaccagtt
    12001 gtccaccacc ttgaactccg accattggtc ataaggaacc tgcatggtcg acatgacgct
    12061 gacatagtca tccaaatcga gtctgacgac ctgtctgaga tactcccgcg gcgtcatgac
    12121 cttgccgtcg gtcgttaccg tctccggcgg gcggcccaga tgcttgtcga ggattcttcg
    12181 aaccgcggcg acgaccagcg aatcgtccca tcggccctcc agcttcacct ttgccagatg
    12241 agctgcgatt tcgtggtacg cagccgcatc ttggagaatc tcatgacctg ctgccagccc
    12301 gcaataagcc tcggccggta ccaccccgta gctccgccag atccgcaaga cggcatcggg
    12361 ctcagagccc ctgttgaaca ccgactgccc ccgcgtgcgg acagattccc gggccttctc
    12421 gacaaattcc cagtagtatg tgtgggcaac ggacagccgc aactcacggc cggtctggcg
    12481 gtggacttct gattccagga acgacgttgc cgagaagtcc cagcactggc cggtcagccc
    12541 ctgcgacacc ggcggctggt gccaggcctg tttgaagtcg gcgggtgatt tcggcgcatc
    12601 gaccgtggac gaatccagca tgagtaccgg ccctggttcc cctggccggg tcgccggttc
    12661 aatccgttgg cccaaacgct gcttgaacgc gtccggatac tcatgccaaa cggctctgtc
    12721 ttgtgcagac gcacatgaat gccacgccgc cagtaccgtc gccacgatcg cccgccgcac
    12781 gatggcgtgt ctcatcagat ggtccttccc ttgccgatag atgccccttc ttgttcgata
    12841 cgtccccgag cttgacgtct gtccgctctg aaaacccgcg gaggacaact cggtcaagca
    12901 aggggaaaac cacttgccgg taacatggaa ggtgatgagc aggccgtcca cctctgccgg
    12961 ctcctgcgcc ccgatgagta ctataccacg agcggggaaa cgacacatcg gtgaatgcaa
    13021 aagcgcctcg cccggtaagg ccgcccgaat gggtgcatgg ccccggccca ccatcttccg
    13081 ccgactatct ggcggaaatc tacgagaccc gctattcttt gcggaacgcg gcaggcagga
    13141 gccccgaaag gggctgcgtc cgtctttgtc gcttgttggc agacatgccg acggatcgtc
    13201 cgacatccga cagcacaaat ggagccacaa tgagcgatgc tgtgacccaa agcgtcagtt
    13261 tccttggtcg gttcaccgtc ctgaagggcg cggctcgcga attgtggatc accttcgcca
    13321 tcaaactgtt gggcataatc gcctacagcg tcatgaacac gacattcgtc ctctggttgt
    13381 cgtacgacct tggctacagc gatgagcacg ccggatattg ggtgggagcg tggtcggcgc
    13441 tgatgacgct gctcaccgtg ctggtcggct ctctgaccga cgcgatcggt ctccgcaaag
    13501 ccttcctgct cggcgtcatc gtctgcgtag gagcccgggc ggtcatgaca ttcacggcca
    13561 tcccgtgggt ggccctggga gccggtttgc ttccgctggc tctcggcgag gccttgggca
    13621 cacctgtgct ggtggccgct atccgccgat actccactac cgctcagcga tcgatttcgt
    13681 tctcgatctt ctacgcaatg atgaacgtcg gctttcttat cgcaagctat attttcgact
    13741 acgtccgcga aggactcggt gagcatggcc ggtttaccct gccgctggtc ggaatcgagc
    13801 tgacgtccta ccgcacgctc ttcctcatca gccttatcgt cgagatcctc atgctgccca
    13861 tcgtctggct tatacgcgag ggcgtcgaag ccaccgacga aggtgtgaag ataactcccg
    13921 gagaacgaaa gggcccgcac gaaaacgtgc tgtccgccct gatactcatg attcgggaca
    13981 ccttgcgcga aagcgcccgg atctttgcgg gcctgtggcg gcagtcaggt ttctataagt
    14041 ttctcgcttt cctggcattg gccgccttcg tccggctgat cttcattcag atgtactaca
    14101 cctatcccaa gttcggcatc cgcgaacttg gcgaaggagc ggcagtcggg cgtctctggg
    14161 ccatcaaccc gtttctcatc atcttccttg tccctcttgt cggcgcgatc tctcagcgaa
    14221 tctccgcata cagaatggtc tccatcggca gcgccatcgc cgccgcgtcg gtgttcatca
    14281 tggccatgcc ccccgcgtgg ttccagcctc tcgcggatgg gcgattgggt catctgatcg
    14341 caaacacctg gctcggaggc ggttcacgct tcacgcctga cgatttcctc gacctcccgt
    14401 cgctgacccg caggtttgcc tctgcttccg atcccgcatc ccttgccgtg agcgaatcgt
    14461 tctcggaggc aacaagagcc ctgcttcatc ggcgggttgc ggtggctgac agaatatccc
    14521 ggtcgactca acatccaagt tccgcgctct tgattgcggc cgacattgcc gatcctgact
    14581 ccttcgtggc gaggttgcag aaggatccgg agccggcaac caggcccgtc tcggactacc
    14641 tgtggggacg gttccccgcc gcccagaggc agaaaatgac cgatgcgaat ctgcccccca
    14701 cgaaaagaat cgctgtgttg ttggaggagt tgaaccgggt actgaaagac gagtccttct
    14761 gcgaaaaaca tcgcgacgtg ttccgcgcta ttcagttgtc ggaagcgacg cgagctttgc
    14821 tgaagctcga cccccgtggc tcggaggatg tcctctccaa tctgtcagta ctccagaatc
    14881 gacttttgct cgaggatgca tacccgtcgc acatcgccgc gagcgaccat ccgttgcggg
    14941 cggcgctcgc acatgatctg tcgcggatga tccgcaagag taccgttcac atccggtttg
    15001 ctgacggacg gcaactcgcc gccacacggc ctgtgctgga ggatgtcttc tccgtcgaga
    15061 ttgcgaggaa tcgggttgga gtccccggta gcgtcagccc ttggtacgtc atgatttttc
    15121 tcttcatcgt tctgctctcg ctcggcgagt ccatctattc gccgcggctc tacgagtacg
    15181 cagcttgcat cgcgcccaaa ggacaggaag cctcctacat ggctctgtcg tatcttcctt
    15241 tctttctggc caagctcttc gtggccacgt tctctggcgt tctgcttgcc cgttattgtc
    15301 ccgaagcagg cgacaggcat tccggtacac tctggctgat catcgcattg ataactacaa
    15361 tcgcgccggt cggtttggta acactgcgcc gatggatccg ggttcccgag gccgggcgcg
    15421 gcgattgaga catggtacga aggcctgacg acccaacgag cccggcaatg agcagacggt
    15481 tgcattcagg gaggtgatgt atgagattct ccaggctttg cgccttgccg gtcatgctcg
    15541 catggtgtgg gcgaaccacc gtgccggccg ctgccgccgc cacctcgatc cccccgacag
    15601 acaaggtcaa aatcggcgtc atcggtcttg tccacggcca cgtctacggt tttctggagg
    15661 atgcggtcag gcgaaccgat gtcgagatcg tcggcttggc tgagcctcgc gaggaactgc
    15721 ttcgccgcta cggcgagagg cacaagttcc ccgagtcgat ccgattcaaa gacctggatc
    15781 agatgctgga tactgcccgg ccgcaggcgg cggccgtgtt caccgacacg ctcgagcatc
    15841 gcagggtcgt ccaagcatgt gccccgcgcg gcattcacgt catgatggaa aagcccctcg
    15901 cgaccacgat ggaacacgcc cgggcgatcg agaaagccgc ccgcgagggc aggatccacg
    15961 ttgtcgtcaa ctacgaaacg acatggtatc cgaataccca ggccgcatac cagttggtct
    16021 ccaaacaggg acgaatcggc cctatccgta agatcgtggc ccacgatggg caccaaggcc
    16081 ccaaagagat cgggatgccc agggagttca tggagtggct caccgacccc gtccgcaacg
    16141 gcgggggggc gttgttcgat ttcggctgct acggggcgaa cctggcaacc tatctgatgg
    16201 gcgagcagcg cccgttgtcc gtgaccgccg tgacccagaa gctcaaatca gaccccgtgt
    16261 acgagcgcgt cgatgacgaa gccacgatcg tgctgaccta tcccacggcc caagtgatca
    16321 tccaggcctc gtggaactgg cccttcaacc gcaaggacat cgagatctac ggagaacagg
    16381 ggcaatatct gacggtcgaa agagatatgt acaggatgag aacacttcat gcagccgaaa
    16441 cgctgaacaa ggccgagccg atcgccgccc cgatgcaaga ctccatctct taccttgcgg
    16501 ccgttgtccg aggtgaggtc ttgccctccg gtccctcctc actgaaggtc aacatgatcg
    16561 ccaccgagat cctcgacgcc gcccgacgtt ccgccgagca tggggttacc atccctctgc
    16621 cggacgtgat gccgtagcct cgacaggcgc taccctggaa gacggtcttg tcggcgggtc
    16681 aaaggagctc gattagtctc ttccgtcgcg actaatgcgc attttgcgcg catgtttctg
    16741 gatcgcagag cgcttgggtt gcggcctgca aaaaggcggt ccgccttagc tccacagggt
    16801 ccgccttggc cggccgtctc tctcctacac cacttcgctg atcagcttca tctccgcgaa
    16861 ccgacagaac cgcccctgat tccccgccag gttcccgtag caggtgacgc gtgaatccca
    16921 gccgtctagt tgcgccggga ctgctcacct gaggcggata agcgccccct ggcccgccgt
    16981 cagccacagg ccgttctcct cgtccagtgc gacaacatgc ccatcttggg cggagataat
    17041 ctccttgctg ccgtatgcct tggcgaatgt gatgcggaga ttgcccgacc gttcgatgct
    17101 cagattgacg atcatgacgt aatcgctgcc ggaatcatcg gtgaactcgc cgatcatgac
    17161 cggcaacgac gtcttcgcga agccccgagg cgagatcgtc gaatgaacct ccttaatgat
    17221 tcgacccggc agcggcggca gcgaatcaag cattcctggc ccgtcaaaga acacccctgt
    17281 ggatttgagg cgattcatga tccgccccag cgtcttgacc tgatggttga ccaactgcag
    17341 gtatccccag gtctcggtct tgttgccggt gtgatcgatc ggcgcatagg cgtaagcagg
    17401 tctcggcccg ctcccgccgt agtaggtgta ccaggacaga ccccggccgc ctgccgccag
    17461 agtggtgtac gcctgaagca tcatgttggc cggtgacgga atggttgtgt tcttgcggat
    17521 ctggttcgac gagacgatgt tccagaaggg cagatcgtgc ttgagtgcga cccggcggac
    17581 ctcaagtaaa tcggcgaaat agttttttgc ccgcccggcg tcaagaagat cgtccgagta
    17641 ctggatcatg tagttgtcgt aactgatgaa ctggggcttg acctcggcgg catatcgttc
    17701 gaggtattcc gtgaaactcg ccgcgcccaa ttgcgacttg tccggggcac cgatggtcgc
    17761 gtaaccgggg aagagattga tgtacgcaag cttccccggt gcgtaccgct tcacggccgc
    17821 gacggctttg gccagcgccc caaactcccg ggtgcccggt tcgtcaacaa tgaaatagcc
    17881 caggacggca gagctgttcc cggccagctt gaccatggtc ctcagacgct ggtcgatctc
    17941 ttcgtccgag agggcccgcc agggccgctt ccatggcacc tcgtcatccg gcggggccat
    18001 gatggcggcc aagcccaatc gttcgcagac cggaaggtct tccgtccgag caaagccgga
    18061 gacggtgaaa ttgcattcgg ccatgctttc aaccccgtac cgagggtttc cgtacggcgg
    18121 cctccacccg tgcaccggac tccagggtag gattgggaag aggtcttccc acaagcttcc
    18181 accagccgtt ccggacgttc tcgccgacgg ctcgcaaccc gcgttcaaag tggccttgtt
    18241 ctgttcttgg gccgcctgag cgcaaatgct gctcgccgcc atcagactga tgattgccgt
    18301 gattgcgact tgcatgccaa tctcctgctg caacgaaacc tctgattgat cgacagtcta
    18361 cgccgccatg ggcgaattgt cagcccgtgg cacgtggtgt ctgggggtgc caagtcgcca
    18421 aaacccatgt atactggccg gggttgattg ccggctcagc cgccgattgc cgggcggcct
    18481 tgttgattca ggccaatgcg tgtcttttcg cagagaacaa ggaggcaatt ccgtgagttg
    18541 tcacacaatc gctttctcaa gatgtctgat gattctgtct ttggccggcc tgctgacggg
    18601 ttgtcacccg acgccggtcg gccccacccg accgcccgct accagcccgg ctcctgtgcc
    18661 caaacgctac tcctatacgc ccacgccgta cccgcttgac gaaagaggct tctcccccgt
    18721 tgacaaggcc ttggccgccg cctacgcctg cgaagagatg atcgcagacg acgccgaggg
    18781 atcactcaat ccctatgtgc tgaaggtcat ttccgcttat cccatcgacg gctcttatcc
    18841 atatcattgc agttggaagc cccgtgagta cgacatttat aacggcgtga cgcaggatat
    18901 gtggtacaag ggcatgatcg tcgcgaaggc ttaccccgac ggctcacggt gcagttattg
    18961 ctgcggactg accttcgaga tcttcatccg ggcaatgaag ctccgtaata tccagaaagg
    19021 gctcgacccc gacgacttca acggaatgac cttccacgac ctcttcaata tgctccagct
    19081 ctggtacatc gaaggcaagg acgacagtga acagcgggcc atcgtcagct acggcctcgg
    19141 gcgggcgatc accgatttcg aggaagtccg ccccggtgat ttcctgagct atagcaccac
    19201 tccgcccggc ggccattcag tgattttcat caactggctc agggacgaaa gcaacaagat
    19261 catcggcctg aggtatttct cgtcgaatct ctccggcagc aagggcccgg gctatggcga
    19321 gggcaggttc agcgactgca acaacggccg tgggctgctg cgcaactcgt tgcgaatcgc
    19381 ccgtgtcggc gcgatcaagg actacaaacc gtttgaccgg ctgaagatcc cgcgacgaaa
    19441 cgcctatgct cccaatcagc cggatcgaat tatatacctg ccggcaacca ggccggccca
    19501 gtgacctgtc ggcggcaagc ttgttcggca ggcacgtgac aacaaggtcg caagtccttc
    19561 catttcgaca ggaggcatgg atgcgatacc tgtttgtggt agcggcggta ttgatggtcc
    19621 tgcccggccg ggcctccggt cagacgccgg acaacctctg caggaacccc gggttcgagt
    19681 tgctcaaccg cgctggcgat aacttcccca tgaactggac ggcggtgcac aaccccgccg
    19741 gtcagggaac ggtgatcatc gacaagacga cccacgctgg ctcgatcgcc gttcgcatgt
    19801 cggccacgcc ggattgctac gtgggcataa actccgaccc gatccctgct actaagggcg
    19861 ttgtgacgtt ttggtatcag attgtccggt ccgactccgg cggggaaaac cttcgactgt
    19921 tcgtgatcgc ggtcgccggt gatgctgccg aggtggttcg cgccggatac gaactgcccg
    19981 tcgaacatgt cggcgatggc cggtggcacc agggacgcgc cgagttcgac ttcagctctc
    20041 acgccggtgt gaatg
//
LOCUS       DAOTPW010000094        20030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2758_length_20030_cov_4.660876, whole genome shotgun sequence.
ACCESSION   DAOTPW010000094 DAOTPW010000000
VERSION     DAOTPW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20030
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2758_length_20030_cov_4.660876"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..925
                     /locus_tag="P5316_10215"
     CDS             182..925
                     /locus_tag="P5316_10215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRR85375.1"
                     /translation="MPEARFHVADLSNTDVVLTEQESRHASHSRRLRPGDPVVLFDGH
                     GTEAGGQIATVSRSGVHVRVLRTWTRPRLRPVVTLAVAMPKGPRQDFLVEKCTELGIA
                     ALWPIQTTRSVSQVSAHKLNKWRRTAIEAAKQSGQAWIPELSPPRQLNEALAQAADFD
                     RLLIAVPDAEISSTWATTVADARSVLAFIGPEGGWTDSERSACLAKGCRAVSLGPNTL
                     RIETAAIALAAILHAAGGKPLQSDNAHGS"
     gene            1157..2566
                     /locus_tag="P5316_10220"
     CDS             1157..2566
                     /locus_tag="P5316_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRR85376.1"
                     /translation="MSGKPTTLRQLRESGWQPKTVKREMHDNFLRMLQSGEELFPGII
                     GYEDTVIPELNIGLLAQHDMLFLGEKGQAKSRLMRLLVRFLDPEIPYLDIPDCPVHEN
                     PYKPITAAGKRFLTEHAEKDVPIAWWPRRQRYAERLSPGTKFADIIGEIDPARLAAGT
                     SLSAEEALHFGLIPRMHRGIFAMNELPELDELVQVGLFNILEERDVQIRGFPVQFDID
                     VLILFSANPATYNRSGKVIPQLKDRIGTVIHTHYPRRREVGIQIMEQEAGICLDGEFP
                     VRVPVFMKEIIEQMSICARRSKYVDQQSGVSARFSIANYRTMVASARHRAVRLGERPA
                     VPRISDLAHLQASSLGKLELDMMGSHQMSERQVLDAILAEAVRIVFDEYVDRHGLEEI
                     TDVFSKGVKIEVGDMLPSSQYAERVKRIPKIWEKAFEVNPAENDAVRASCVEFVLAGL
                     YAKDRISRSQHHGRITYEL"
     gene            2556..4280
                     /locus_tag="P5316_10225"
     CDS             2556..4280
                     /locus_tag="P5316_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85377.1"
                     /translation="MSSDRIARSNDRQPLGGVIHTYQKFNPKSFPSPTSPPPDVVSPA
                     FQHMLVYGSMRELTEEELARAIHIDPRQIASLGPSLESLIAMLLERKRKILATYETET
                     VQQEVRDRWQKEYRRVRMPKPLTGTYYAAVRGEQLYDLERLWYRIERKRPDIAARLLT
                     LMDLLGQKYQVDELAAKYEFTGRTPMDVPAALTVKEELEAIDRLLEQLRKAAETGQIG
                     LIDMEALAEFAEPGDMERLNALQQYIQDYLRELAERQGLEKTEGGYRLTPKAMRLFQS
                     RLLQEIFSDLKAARSGRHTGPIIGEGPVEIQQTKTYEFGDSLTHMDIPASMINAMIRQ
                     GPGVPVRIGPDDIEIHRTRNNPKCATAVVIDMSGSMRYGGQYINAKRMGLALHGLIRT
                     EYPGDFLQFIEMYTFAKPRHISELPGLMPKPVTVTDPTVALKADMSRPELTEFDIPPH
                     FTNIQHALQLARQYLSVQDTPNRQVVLLTDGLPTAHYDGNFLYLLYPPHPLTEEATLR
                     EAGRCAQEGITINIFLLPNWAQSSEDVQFAHRLAESTRGRVFFTGGRDLDRYVLWDYV
                     RRRRLMIA"
     gene            4336..6063
                     /locus_tag="P5316_10230"
     CDS             4336..6063
                     /locus_tag="P5316_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRR85378.1"
                     /translation="MSPFCCCCVSLGLVCLTFASLDVSAAEPPAADAARRFIAEYEAS
                     IRPLQIASALADWNAQASGKDEDYKAAQEAQNKLDAALSDPAAFARLKQVREDLGKVQ
                     PKPDSLLARQIELLYLEYAAKQIDTELLRRITAKASACMQAFNVYRAKVNDRELPDSE
                     VRKVLRESRDSAYRRAVWEAAKGVGSVVEKDLKELVKLRNQAARKLGFTDFYEMQLQF
                     AEMTKSEVLKLFDELHELTREPFREAKSRLDAGLAADYGIPVAELRPWHYHDPFFQEP
                     PATYTDHLDPIYQSADVVKLATEFYAGIDLPIDDVIARSDLYERPGKYPHAMCMDVDR
                     DGDIRIMANVVPNHQWMSTMLHELGHAVYDKYIPRSVPYVLRTPAHSFTTEAVAMLFE
                     RVADQPAWMQSVGLKVHDPKAIAETTDRMHRDHVLIFAGWSQVMVRFEAALYADPDQD
                     LNRLWWDLVEKYQLVNRPEGRNTPDYASKIHIISSPVYYHSYMMGEMFASQLHHTIVR
                     DVLKANTPTPLYSGRKAIGDFMKEKVFGPGASLRWDALVRHATGRDLSPKTMMAEIGG
                     DERMSATAD"
     gene            complement(6076..7110)
                     /locus_tag="P5316_10235"
     CDS             complement(6076..7110)
                     /locus_tag="P5316_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR85379.1"
                     /translation="MELPERIKTEQELEEVMTRPSPGLVEDMVRVEGDLLLLGVAGKM
                     GPTMARLARRAWDQAGRESRIIGVARFTDAAVRDRLEADGIETIAADLLDEEALASLP
                     DVPNIIYLAARKFGSTGNEPLTWAMNTYLPARVAVRFRCSRIVALSTGNVYAFSPTDG
                     PGPDESTPVGPVGEYAQSCLGRERMFQYFSEQNGTPVSLIRLNYAIDLRYGVLMDVAT
                     KVFTGQPIDLTMGHVNVIWQGDANAAILRSLRYCACPPFILNVTGPETISVRMLAAEF
                     GRRLGKEPVLTGTEAPTALLSNAARYHKMMGPPQVPLERMIDWVAHWVSIGGRTLNKP
                     TKFQSRDGRF"
     gene            7637..8680
                     /gene="tsaD"
                     /locus_tag="P5316_10240"
     CDS             7637..8680
                     /gene="tsaD"
                     /locus_tag="P5316_10240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRR85380.1"
                     /translation="MYGSDNAPIILGIETSCDETSAAVVIGSHKVLSNVVATQFDVHA
                     KYGGVVPELASRAHIENLDGVIQEAMARAHVTRQDIDAIAVTNRPGLVGCLLIGVTAA
                     KTLALAWSKPLVAVNHVHAHACSPAIDLPPNNPEPWPAVALVVSGGHSSLFLVRDPLN
                     IELLGATIDDASGEAFDKVATVLNLGFPGGPIIERIAREGNPRACKFPRTMLGRDSLD
                     FSFSGLKTAVLYRVHGPGRTTGGLERLSRQDVADIAASFQEAVVDVLVAKTMLAVEKT
                     GVRTVMVGGGVAANGQLRTALSTACSQREISLYLAPMIYCTDNGAMVGALAGYFLRAG
                     RTDSLAVDPRALM"
     gene            8721..11951
                     /locus_tag="P5316_10245"
     CDS             8721..11951
                     /locus_tag="P5316_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85381.1"
                     /translation="MTGEVHAIGSSRKHVAILLSVFLFAVVCGCAPTPVLINPGFEIA
                     SPEGTLPGWTILTGLDPHGYGPPAFDRRFDDVAPAQGRRGYRSDHCLSFPSDGAWACP
                     VFAHSNGDGQGINGKLLGKAAAFQTVNASPGRYRLSAMLRTADGHLFSASFSLGVNIG
                     PPVAYANDDSTGIRWTSHDLAMARDALRGVRERGEWCRYESEPFSIDRFGPVTVWIRF
                     NYANENQFLTRWQVDDVAIEPLRTDAEMVRPTAVNCPPVPEPLRMREFCGDQDAFLAD
                     AGGSVVVEAPEIRLFRKARQIPPGARVTYRFPTRQMAGPLNIFVSAAGPARVRVHNSL
                     SELTFNAGGTDPKRPVACEWKVPGRVVGGEETCVTVEPRGQQSVRLYEIEMGLRARTI
                     CRLLRVEADAVSVPWVIGSWDASSAEFAEKPNQQSWPSSSTQHLVPLPVSNHSVAMES
                     AATPSGRWKLRFMHKPARGHRYYLVHGLIGGASSLDVGDDGIIDWKSENKGEEIVDFD
                     ATDLLVPGMNTVIIDATGQYDFAAMIDVCPGATDLSRLQLVFEGDQLAERLTRVADNT
                     WFWLRELHYEPNGFVDASIPKGHWYGQFWPVDIAFALREWIGWGRHEETVAIGRFMAH
                     YGWHGHPSNRSGGVDNTAGNILATQLCEIIRRCDFDPHVTEVIWKTISAYADEVAAKA
                     NDNPYGLIRGTNWENAGNREHGPCYALSTTLGAAAALRKAAAIAESLGDPTAAGQWRE
                     LAANLRSATLKHLVLQQDHKCPTGFVLPKGTWAYGLRVDGTIEDQPLAGYFWAGGAPI
                     EVDGLMPPADRELYDVYDRTLQLAVPLMERKARGVVSRYADSYDGPDALIAVAALCDR
                     IDSLQPLLEGLARDTDVLDDRGSEFAELSRWAGGGPGGPEDTNLVCAAGFLYPLRILA
                     GIDDLLADGRQLSLFPRLPWNWTGLRVNNWPVRCRDADGRETWTSLTYSVRRSDAYCG
                     IKVHTTHWVPGAPIRLGPFPPTGKALPLTVNGKPTDARLHVSGDAAWLAVEVDVGPAD
                     TVIEAGYPCDGCSADRGVPASKRLYGMTSSRRGLPSMVRFTLPSRP"
     gene            complement(11882..13285)
                     /locus_tag="P5316_10250"
     CDS             complement(11882..13285)
                     /locus_tag="P5316_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRR85382.1"
                     /translation="MFRLTMWVMLIVGVVPGVSVLAGPSAEDLLRVEQLQNTLAAVAE
                     EVRPSVVAIRALRRFAAPHADASVTTLPSGRGRSVHGVLVPSIGSGVIIDRQGLILTN
                     EHVVHDVDPDRIECILSTGDRYIVQGMTTDPRSDLAVLSIDAENLKPAVWGDVESLQQ
                     GHFVIVMGNPLGTAFDGDGRAAMSFGIVSALGQDLTQKLDPDRYYGNLIQTDARINPG
                     NSGGPLVNLKGEVVGITTAVSSRSGGSDGVGYAISLGKDTRGIIDQLVDGQEVRYGYI
                     GVHLQDRNRPRIGGRASARIVISSVEPESPAQRAGLREGDAILRFDNKPVANADELVR
                     LVAMSGIGKAVPLSIVRDGQSLDLDVVPGLRPAPARGVNIEPDFTWRGMTFSVANSET
                     RARFALPESATGPVVSVVVAGSPADKAGFRPGDVIAAVNGQPVGGFRRFRRWIQGLEG
                     KVNLTIEGKPRRELVMP"
     gene            14041..16470
                     /locus_tag="P5316_10255"
     CDS             14041..16470
                     /locus_tag="P5316_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRR85383.1"
                     /translation="MDLRLPFVRTVGVAVAACYVLTIGACHDDGRRVEWITSESAEFT
                     ITVAGTADHHRMGPGWKSDPGSTHNPTRLLIINNRGDRSVINPRIVVDGAPDWFDIHH
                     IVCQIVRPGMTDEEKAFALWQWIRRNLSDGPTYGSPLWGDTRSMTRFMNACGTGACGT
                     YHIVMPVIGKHAGLRTFSGCFADCSHAVQKEFFDGQERFLDAHIPHEEGQPGGWFALK
                     LNNAELAGVDEIMEDRHLIDRAGAGPGRAGYVAYFGPGCGFHEEKPALDPRDMGMILR
                     PGESIAWQWTMHAPPWQKGDQTSRADRHGSGYVKFRPRLSQEQVRYCADTTENLHFIE
                     RQGDEAVVPLDVSKAATVVYRLYCPYMMTRAAVEGRFETGDAGTATIEYSFDGRDYRP
                     AWRSTSRGMHEPQVELPDDDRFHEPVFTHELWFRITLEGRQTCLSDLRLRGHFQAYRP
                     SLPSLHAGSNRIRFSGRFDPDPGDNPALAKAVVVDYWWRDLPDLPVPRPPPAPVHPAP
                     GGQFGFNETLRWRPAEVAGQAGIAGYEVYVSARPDLAWPVMPNTHRLIGSPDPATALV
                     SPDVLRHGATYYWRVRGRSTDGMWGDWSPTWSFVAAGPGVPRDLKVTYDQPTGATSLS
                     WSPPAEGSPVDHYEIYGSSEHGFSPLRSNEEARVHGGVVRRPKTLVEVTRDSHWDASA
                     RTEVFYRVVAVDMQGNRSAPTAVVKVPSPALLPVDLPPARVGHEYRFKLPARLRTGRF
                     ALSLRKGIITDGADTPRFALADIESLRIDPSTGELNGTPMVVGNHILKVHLEDGRGGK
                     CDRQYALVVQP"
     gene            16609..17757
                     /locus_tag="P5316_10260"
     CDS             16609..17757
                     /locus_tag="P5316_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR85384.1"
                     /translation="MAAALAAVGFWCAQASAQQAEKFNGLHLSLGNLSRLSDAKTRSI
                     SPENFTGEKGKGGMATDGTGKNAARELGQGWKISPSVRIKANTTFTLADIKGSGAIQQ
                     IWMTPSGHWRFLILRIYWDGETEPSVEVPVGDFFACGWNMYAQVSSLAVCVNPGSAFN
                     CYWVMPFRQSARLTLENLSDKDETVYYQVNYTLTRVPEDEGYFHAQFRRNNPLPYKQD
                     HVILDGVKGKGHYVGTYMAWQSNNVGWWGEGEIKFFMDGDKEFPTICGTGTEDYFCGS
                     YGFHAKTADGKTEYREYTTPYAGMPQVIRPDGLNESQQRFGLYRWHIMDPVRFESDLK
                     VTMQALGWRSGGRYLPLQDDIASVAYWYQTEPHAPFPKLPDKDGLEVN"
     gene            17910..19256
                     /locus_tag="P5316_10265"
     CDS             17910..19256
                     /locus_tag="P5316_10265"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85385.1"
                     /translation="MDKQERIGRRNVLKCSAAGLAALSALSGAMSGCNAKSERPAVAP
                     KQQSRTPPGERVVVGVIGTGGLGLGHHIRGCLLPNDNFEVAAVCDVDRNHRDAAARMV
                     MEKTGKRVAAYEDFRHLIDRKDIDAVLIATPDHWHTIPAIMAAEAGKDIYCEKPLTLT
                     VNEGKALVAAVRRYGVVFQTGSQQRSDNAFRKACELVRSGAIGKLKRIDTVLHPVPKG
                     QWQPVTTPPPSLNWNFWLGQAPYVDYSPNRVHYQFRWFYDYSGGVMTDWGAHHNDIAQ
                     WANGTDGTGPVWVDGTRATFAESGMHTVPLTFDVHYKYANGVELWCHTNDQTYDDGTK
                     FGNGVKFTGENGWIFVARGNRLQASDPDILDVEPAVKLYNSSNHHRNWLDCIRSRQRC
                     ICDVEIGHRSVSICHIGNISMRLGRALNWDPATEQFVADEAANRMLSRPMRAPWHL"
     gene            19389..>20030
                     /locus_tag="P5316_10270"
     CDS             19389..>20030
                     /locus_tag="P5316_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85386.1"
                     /translation="MASIRELIVQFSGEQEAAYKAYRQVELMVAKANAPGSEAERTQL
                     AAELTEQLWALSEAKKDDKGNDLPRTPTHPVATRIKILRLLQGMAGDKEVPAIAKALD
                     ELELREDARCTLDRISSHESDKALVNALDAAVGAEFRVGIVSSLAARPNAYVMAALQK
                     ATADCDPEVALAAVEALANFPEPANDEFIANAARCSCPVARRRALSARVRFAEN"
BASE COUNT         3945 a         6586 c         5864 g         3635 t
ORIGIN      
        1 cctcggggct catttcagcc gcggaaacaa ggcctgcatc attttcctgc ttgttatctg
       61 acatgctcag cgtctgctcc aagccaacca aatcccgctc tgatcaaggc ctcactactc
      121 atcggtcatg gtgttggacg gcttcattgt cacccttcaa cggggccccg ataatacctg
      181 catgcccgag gcaaggtttc acgttgccga tctcagcaac acggacgtgg tgcttaccga
      241 gcaggaaagc cgccatgcaa gccactctcg ccgcctccga cccggcgacc cggtggtgct
      301 tttcgatggc cacggaacag aggccggtgg tcagatcgcc accgtgtctc gctcgggcgt
      361 acatgtgcgg gtcctccgga cttggacccg accacggctt cggcctgtcg tgacgctggc
      421 cgtcgcgatg cccaaaggcc ccaggcagga ctttctggtg gaaaagtgta cggagctcgg
      481 catcgccgcc ctgtggccta ttcagactac ccgcagcgta tcccaagtct ccgctcacaa
      541 gctgaacaag tggcgccgaa cggccatcga agcagccaaa cagtcggggc aggcttggat
      601 tcctgaactg agcccgccgc ggcagcttaa tgaagccttg gctcaggctg ccgacttcga
      661 ccgactgctc attgcggttc cggatgcgga aatctcatcg acatgggcca ccacggtcgc
      721 cgacgcccgg tctgtgctgg ccttcatcgg tcctgaaggc gggtggaccg actctgagcg
      781 gtccgcatgc ctcgccaaag gctgccgcgc cgtctcactg ggccccaaca ccctgcgcat
      841 cgagactgcg gccattgccc ttgctgccat cctgcatgcc gccgggggta aaccactgca
      901 gtcggacaac gcccacggta gttgaggctt cctgaggtgt ccaacaccct gaccctcggt
      961 ctgccgctct gccgcttcct gtccgacggc tgtcgttcga tctctctaaa tgccccatgc
     1021 ttggccggcc tcccccgaac gtcctattat tatgttgatg acgaccggcg accatggcag
     1081 gcatgccgat tccggacgga gccttgatcc ggcgtctctg tacaaggatt cgggccgaca
     1141 aagggggccg aagcacatgt ccggcaaacc aaccacattg aggcagctcc gcgagagcgg
     1201 ctggcaaccc aagaccgtca aacgcgagat gcatgacaac ttcctgcgaa tgctgcaaag
     1261 cggcgaggag ctctttcccg gcatcatagg atatgaggac accgtcatcc ccgaactcaa
     1321 tatcggcctg cttgctcagc acgatatgct gttccttggc gagaaaggcc aggccaagag
     1381 ccggctcatg cgcctcttgg tgcgcttcct cgaccccgag attccatacc tggacatccc
     1441 cgactgtccg gtgcacgaga acccttataa gccgatcacc gcggctggca aacgctttct
     1501 gaccgagcac gccgagaagg atgtgccgat cgcctggtgg cccagacggc aacgctatgc
     1561 cgagcggctc agcccgggca cgaaattcgc cgacatcatc ggcgaaattg accccgccag
     1621 gctcgccgcg ggaaccagcc tctccgccga ggaggccctg cacttcggcc tcatcccccg
     1681 catgcaccgc gggatcttcg ccatgaacga gctgcccgag cttgacgagc tcgttcaggt
     1741 cggattgttc aacattctcg aagaacgcga cgtccagatc cgcggcttcc ccgtccaatt
     1801 cgatatcgac gtgctgatcc tgttctcggc aaacccggcc acatacaacc gcagcgggaa
     1861 ggtgatcccg cagctcaagg accggatcgg cacggtcatt cacacgcact acccgcgacg
     1921 ccgcgaggtc ggtatccaga tcatggaaca ggaagctggt atctgcctag atggcgagtt
     1981 tccggtacgg gtgccggtgt tcatgaagga aatcatcgag cagatgagca tctgtgcccg
     2041 gcgatcgaag tatgtcgacc agcagtcggg cgtgagcgct cgcttcagta tcgccaacta
     2101 ccgcaccatg gtcgccagtg cccggcatcg ggccgtgcgc cttggtgaac gtccggccgt
     2161 cccccgcatt tcggatctgg cgcacctgca ggcctcgtcg ctgggcaagc tggagctgga
     2221 tatgatgggc tcgcaccaga tgtccgaacg ccaggtgctg gatgcgatcc tggccgaggc
     2281 cgttcgtatc gtgttcgatg agtacgtcga taggcatggc ctggaggaga taaccgacgt
     2341 cttcagcaag ggcgtgaaga tcgaagtcgg tgacatgctg ccctcctcac aatatgccga
     2401 gcgggtgaag cggatcccga agatttggga aaaagcgttc gaggtcaatc cggccgagaa
     2461 cgatgccgta cgcgcctctt gcgtcgaatt cgtgctggcc ggactgtacg ccaaggaccg
     2521 gatttcgcgc agccagcatc atggcaggat cacttatgag ctctgatcgc atagcccgct
     2581 cgaatgatcg gcagcctctc ggtggagtga tccacaccta ccagaagttc aacccgaaga
     2641 gcttccccag cccgacatcg cccccgccgg acgtcgtttc cccggcgttt caacacatgt
     2701 tggtctacgg cagcatgcgc gagctgacgg aagaagagct ggcccgtgcc atccacattg
     2761 acccccggca gatcgcctcg ctggggccaa gccttgagtc gctgatcgcc atgctcctcg
     2821 agcggaaacg caagatcctt gctacctacg agaccgaaac cgttcagcag gaggttcgcg
     2881 accgttggca gaaggagtac cgccgcgtcc gcatgcccaa gcccctgacc ggcacgtact
     2941 acgccgccgt tcgtggcgag caactctatg acctcgagcg gctttggtac cggatcgagc
     3001 ggaagcgtcc cgacatcgcc gcccggctgc tgacattgat ggatctgctc gggcaaaagt
     3061 accaggtgga cgagctggcc gcgaaatacg agttcaccgg ccggacgccg atggacgttc
     3121 ccgccgccct gaccgtgaag gaggaactgg aggcaatcga ccgcctcctg gaacagctca
     3181 gaaaggccgc ggaaaccggg caaatcggcc tgatcgatat ggaggctctt gctgagtttg
     3241 ccgagcccgg cgatatggag cgtctcaacg ccctgcagca gtacatccag gattacctgc
     3301 gcgaactggc cgaacgtcag ggcctcgaaa agaccgaggg cggctaccgc ctgacgccca
     3361 aggccatgcg gttgtttcaa tctcggctgc tccaggagat cttcagcgac cttaaggccg
     3421 cccgctccgg ccggcacacc ggaccgatca ttggcgaagg acccgttgaa atacaacaga
     3481 ccaagaccta cgagttcggc gactcgctga cccacatgga tatcccggcc tccatgatca
     3541 acgccatgat tcgtcaaggt cccggtgttc cggtacgcat aggacccgat gacatcgaaa
     3601 tccaccggac gcgcaacaac cccaagtgcg ccactgccgt tgtgatcgac atgagcggtt
     3661 ccatgcgcta cggcggccag tacatcaacg ccaaacgcat gggcctggcg ctccatggcc
     3721 tgatccgcac cgagtacccc ggcgacttcc tgcaattcat cgagatgtat accttcgcca
     3781 agccgcggca tatctccgag ctgcctggcc tgatgcccaa gccggtgacc gtcaccgacc
     3841 caacggtcgc cctgaaagcc gacatgagcc gaccggagct gaccgagttc gacattccgc
     3901 cgcatttcac caacatccag cacgctctgc agcttgcccg gcaatacctc agcgtccagg
     3961 acacacccaa ccgtcaggtc gtcctcctga ccgacggcct gccgacggca cactacgacg
     4021 gcaacttcct gtacctgttg tatccgcctc acccgctgac cgaagaggca accttgcggg
     4081 aagccggacg ctgcgcccaa gagggcatca cgatcaacat ttttctgctg cccaactggg
     4141 cacagtccag cgaagacgtg cagtttgccc accggcttgc cgaatccacc cgcggccgtg
     4201 tgttcttcac tggcggccgg gacctcgatc gttatgtcct ctgggactac gtccgccgcc
     4261 gtcgcctgat gatcgcataa gctccgagca tgcggtatca tgttccccgg gtccttcaag
     4321 acgaggagaa taccgatgag tccgttctgt tgttgctgtg tatcgctcgg cttggtctgt
     4381 ctcacttttg catcgctcga tgtctctgcc gccgagccgc cggctgccga cgcagccaga
     4441 cgcttcatcg ccgaatacga ggcgtccatc cgtccccttc agattgcctc ggccctggcc
     4501 gactggaatg cccaagcctc cggcaaagac gaggactata aggccgccca ggaagcccag
     4561 aacaagctcg acgccgcctt atctgatcct gcggccttcg ctcgcctcaa acaggtgcgc
     4621 gaggatttgg gcaaggtcca acccaagccc gatagcttgc tcgctcgcca gatcgagctg
     4681 ctgtacctcg aatacgcagc caagcagatc gatactgaac tgctcagacg catcaccgca
     4741 aaggcaagtg cctgcatgca ggccttcaat gtctatcgcg cgaaggtcaa cgatcgcgaa
     4801 ctgcccgaca gcgaggtccg caaggttctt cgcgaatcac gcgacagcgc ctatcgccgg
     4861 gccgtctggg aggctgccaa aggcgtcggc tcggtcgtcg agaaagacct caaggaactc
     4921 gtcaagctcc gcaaccaggc cgcccgcaag ctcgggttca ccgatttcta cgaaatgcag
     4981 cttcagttcg ccgaaatgac caagtccgag gtcctcaagc tcttcgacga actccatgag
     5041 ctgacccgcg agccgttccg cgaggccaag agccgactcg acgccggact tgccgctgat
     5101 tacggcatcc cggtggccga gctacgcccc tggcattacc acgacccgtt ctttcaggag
     5161 ccaccggcca cttatacgga ccaccttgat cccatctatc aatccgccga cgtcgtgaag
     5221 ctggccacgg agttctatgc tggcatcgac ctccccatcg atgacgtgat cgcccgcagc
     5281 gacctctacg aacgccccgg caagtatccc cacgcgatgt gcatggacgt tgaccgcgac
     5341 ggcgacattc gaatcatggc caacgtcgtg cccaaccatc agtggatgtc caccatgctc
     5401 cacgaactgg gccacgccgt ttatgataaa tacatcccgc ggtctgtgcc ctacgtcctg
     5461 cgaactccgg cgcattcatt taccaccgaa gccgtcgcca tgctcttcga gcgggttgcc
     5521 gaccaacccg cctggatgca gtcggtcggc cttaaggtgc acgaccccaa ggccatcgcc
     5581 gaaacgaccg accgcatgca ccgcgatcat gtactcattt tcgccggctg gtcgcaggtc
     5641 atggttcggt tcgaagcagc cctctatgcc gaccccgacc aggaccttaa tcggctctgg
     5701 tgggacctcg tcgagaaata ccagcttgtg aaccggccgg aaggacgcaa tacccctgac
     5761 tacgccagca aaatccatat catcagcagt ccggtgtatt accacagtta catgatggga
     5821 gaaatgttcg cctcacagct tcaccacacc atcgtccgcg atgtgttgaa ggcaaacacg
     5881 ccgacgccgc tctacagcgg ccggaaggcg atcggcgact tcatgaaaga gaaggtcttc
     5941 ggccccggcg caagcctgcg gtgggatgca ctcgtccgcc acgccaccgg ccgggacctc
     6001 agccccaaaa ccatgatggc cgagattggc ggcgatgagc gtatgtccgc aacagcggat
     6061 tgacctcgcg ttgcctcaga acctcccgtc ccggctctgg aacttggtgg gcttattcaa
     6121 cgtgcgcccg ccgatgctga cccagtgggc aacccaatcg atcatccgct ccagtggcac
     6181 ctgcggcggg cccatcatct tgtgatacct ggccgcgttg ctgagcaggg cggtgggggc
     6241 ctcggttccg gtcaggaccg gctccttgcc cagccgacgc ccgaactcgg cggcaagcat
     6301 ccgaaccgaa atcgtctcag gaccggtaac gttcaggata aacggcgggc aggcacaata
     6361 tcgcagactc cgcaggatcg ccgcgttcgc gtcgccctgc catatcacgt tcacatgacc
     6421 catggtcaga tcgatcggct ggcccgtgaa caccttcgtt gccacatcca tcagaacgcc
     6481 gtatcgcaga tcgatcgcgt agttcagccg aatcagcgag accggcgtgc cgttctgttc
     6541 gctgaagtac tgaaacatcc gttcgcggcc aaggcacgat tgggcgtatt cccccaccgg
     6601 gcccaccggc gtcgactcat ccggtcccgg cccgtccgtc gggctgaacg cgtaaacgtt
     6661 gcccgtcgac aatgcgacga tccgcgagca acggaacctc accgcaaccc gagccggcag
     6721 ataggtgttc atcgcccagg tcagcggctc gttgcccgtc gagccgaact tgcgcgccgc
     6781 cagatagatg atattcggca cgtccggcag cgacgccagg gcttcctcat ccagcaaatc
     6841 ggcggcaatc gtctcaatgc cgtcggcttc gagccggtcg cgaacggccg catcggtgaa
     6901 ccgggcgacg ccgatgatcc gtgattcccg ccccgcctga tcccacgccc gccgggccag
     6961 ccgagccatc gtcggcccca tcttgccggc cacaccgagc aacagcagat cgccctccac
     7021 gcgaaccatg tcttcgacca gtcccggcga cggccgggtc attacttcct ccaactcttg
     7081 ttccgttttg atccgctcgg gcagttccat gtgtgatggc ctccagtcag gcattggtcg
     7141 tgagtgtacg acagcgcaag cctaccgcca acaggaaaag gctctgtgac gcaggccatc
     7201 ctcgcggttg acgcggttag cgcgaacgca aacttttgaa gcagaccgct tcagcagttc
     7261 ttcccgaccg gcaaatgggc cggccgtttt acgactggtt catggttggg taattcctac
     7321 cttcctggtt gtgcgtttct tagcccgttg aaacgggctg acaatatgaa aacgacgaag
     7381 agttgcgggc gacagcccac cggccataga acgaccggcc tatcagcccg tccgccttcg
     7441 gcgagatctg ccgacctttg gcgggaaacc cccctgaagg gagttgatcg ccaaacctgc
     7501 agcggtcgac atgcccgccc agactgtcac acactccccg cagcaaggca agtcccccgg
     7561 gctggattcg accccggatt caggcgaaaa tgcgcgcctg tctgagcaga gacggacttc
     7621 acggagcttg accggcatgt acggatctga caacgcgccg atcatcctgg gcattgagac
     7681 ctcctgtgac gagaccagcg ccgccgttgt catcggctcg cacaaggtct tgtccaacgt
     7741 cgtcgccacc cagttcgatg tccatgccaa atacggcggc gtcgtccccg agctggcctc
     7801 gcgagcccac atcgagaacc tcgacggcgt cattcaggag gccatggccc gcgcccacgt
     7861 cacgcggcag gatatcgacg ccattgcggt gaccaatcgg cctggcctcg tcggctgcct
     7921 gctgatcggc gtcaccgccg ccaagaccct cgccttggca tggagcaagc ctctcgtcgc
     7981 cgtcaaccac gtgcacgccc acgcttgcag cccggccatc gacctgcccc ccaacaaccc
     8041 cgaaccctgg cctgccgtag ccctcgtcgt cagcggcggc cacagcagcc tgtttctcgt
     8101 ccgagacccc ctgaacatcg agttgctcgg ggcgaccatc gatgacgcat ccggcgaggc
     8161 cttcgacaag gtggccacag tgctcaacct cggtttcccc ggtggcccaa tcatcgaacg
     8221 catcgcccgc gagggcaacc cacgggcctg caagttcccc cgaaccatgc tcggccgcga
     8281 ttcgctcgat ttcagcttca gcggtctgaa aaccgcggtt ctgtatcgcg tccacggccc
     8341 cggcagaacc accggcggcc tcgagcgatt gagccggcag gacgtcgccg atatcgccgc
     8401 aagcttccag gaggccgtgg tcgacgtgct ggtcgccaaa accatgctcg ccgtcgagaa
     8461 aacaggagtc cgcacggtca tggtcggcgg gggggtcgcg gccaatggcc aactgcgcac
     8521 cgctctctca accgcctgca gccaaagaga aatcagcctg tacctggccc ccatgatata
     8581 ctgcaccgac aatggggcta tggtcggcgc attggctggc tacttcctcc gggccgggcg
     8641 aaccgactca ctcgctgtcg atccacgggc gctcatgtga aaccgagggt tgagaattgc
     8701 gaatggagaa ctggcaattg atgacaggag aagtccacgc catcggatcc tccaggaagc
     8761 atgtcgccat actgctgtcc gtctttctgt tcgcggtcgt atgtggatgt gcgcctacgc
     8821 cggttctgat caacccgggc ttcgaaatcg caagccctga gggcacgctt cccggttgga
     8881 cgatcctgac cggcctcgat ccccatggct atggcccgcc ggcatttgac cggcggtttg
     8941 atgacgttgc acccgcgcag ggccgacgcg gctatcgatc cgaccattgc ctgtcgtttc
     9001 cctcggacgg agcatgggcc tgtccggtct ttgctcacag caacggcgac ggccagggaa
     9061 tcaacggcaa gctcctcggc aaagcggcag cctttcagac tgtcaatgcg tctcccggcc
     9121 gctaccgact cagcgcaatg ctgcgcacgg ccgacggtca cctcttttcc gcgtccttct
     9181 cgttgggcgt caatatcgga ccgccggtcg cctacgccaa cgacgacagt accggcatcc
     9241 gctggacaag ccacgatctg gccatggcaa gagatgccct gcgcggtgtc cgcgaaaggg
     9301 gcgagtggtg ccgatacgaa agcgagccgt tcagcatcga tcgcttcggc cccgtcaccg
     9361 tatggatccg cttcaactac gccaacgaaa accagttcct cacccgctgg caggtcgacg
     9421 atgtggcgat tgaaccgctc aggacagacg ccgaaatggt ccgaccgacg gccgtcaatt
     9481 gcccgcctgt tccggaaccc ttgcgaatgc gtgagttctg cggagatcag gatgcctttc
     9541 tggccgatgc cggcggttca gtcgtcgtcg aggcccccga gatcaggctc ttccgcaaag
     9601 ctcgccagat cccacccggt gcccgcgtca cctaccgttt cccgacccgg cagatggccg
     9661 gaccgctcaa cattttcgtc tccgctgctg gaccggctag ggtacgtgtc cacaattctc
     9721 tgtccgagct gacgttcaat gccggaggaa ctgatcccaa gcgcccggtc gcatgtgagt
     9781 ggaaggtgcc agggagggtc gttggcggcg aagaaacatg cgtcaccgtc gagccccgtg
     9841 gtcaacagtc cgtgcgcctc tacgaaatcg aaatgggact gcgggcgcga acgatatgcc
     9901 ggctgctccg tgtcgaggca gacgctgtgt cggttccctg ggtgatcgga agctgggatg
     9961 cttcatccgc cgaattcgcc gaaaagccca accagcagtc ttggccgtcc agctctacac
    10021 aacatctggt cccgctgcct gtatcaaacc acagcgtcgc gatggaatct gccgcgaccc
    10081 cctccggccg ctggaaactc cggttcatgc acaagcccgc tcgcggccat cgctactacc
    10141 tcgtgcatgg cctcatcgga ggagcgtcgt ctcttgacgt cggcgacgac ggcatcatcg
    10201 actggaaatc cgagaacaag ggcgaagaga tcgtcgattt cgatgcgacg gacctgctcg
    10261 tccccgggat gaacaccgtg atcatcgatg ccaccggcca gtatgacttc gccgccatga
    10321 tcgacgtctg ccctggtgca accgacctct cccgcctgca gctcgtattc gaaggcgacc
    10381 agctcgccga gcgactgacc cgcgtggccg acaacacctg gttctggctc cgcgagttgc
    10441 actacgaacc gaacggcttc gtcgacgcct cgatccccaa gggccactgg tacggccagt
    10501 tctggccggt ggatatcgcc ttcgctctcc gcgaatggat cggctggggc cgccatgagg
    10561 aaaccgtggc aatcggccgg ttcatggcac actacggttg gcacggacat ccctcgaacc
    10621 gctccggcgg tgtcgacaac acggccggca acattctcgc cacacaactt tgcgagatca
    10681 tccgccgatg cgacttcgac ccccacgtca ccgaggtgat atggaagacc atctccgcct
    10741 atgccgacga ggtcgccgcc aaggcaaatg ataaccccta cggcctcatc cgcggcacca
    10801 actgggaaaa cgccggcaac cgcgaacacg gtccctgcta cgcactctcc accacgttgg
    10861 gagcggccgc agccttgcga aaagcagccg caatcgccga aagcctcggc gaccccacgg
    10921 ccgccgggca gtggcgcgag ctggccgcaa acctgcggtc cgcaacattg aagcatctgg
    10981 tcctgcagca ggatcacaag tgccccacgg gcttcgtcct gcccaaaggc acatgggcct
    11041 acggccttcg cgtggacggc acaatcgagg atcagccctt ggccggatac ttctgggccg
    11101 gcggcgcgcc gatcgaggtc gatggactga tgcccccggc cgaccgcgaa ctctacgacg
    11161 tctatgaccg cacccttcaa ctcgccgttc cgctcatgga acgtaaagct cgcggcgttg
    11221 tatcgcgata tgcggattca tacgacggtc ccgacgcgct catcgccgtc gctgcactct
    11281 gcgaccgtat cgactccctc cagccgcttc tcgagggcct cgctcgcgac acggatgtac
    11341 tcgatgatcg aggaagcgaa ttcgccgagt tatctcgctg ggccggcggt ggaccgggcg
    11401 gccccgagga tacgaatctc gtctgtgccg ccggctttct ctacccgctt cggattctgg
    11461 cgggtatcga cgacctgctc gccgatgggc gacaactgtc cctcttcccg cgattgccgt
    11521 ggaactggac cggtctccgc gtcaacaatt ggcccgttcg ctgccgcgac gccgacggcc
    11581 gtgaaacctg gacgagtctc acgtacagcg tccgtcgcag cgacgcgtac tgtggaatca
    11641 aggtgcatac cacgcattgg gttccgggcg cgccaatccg gctcggtccg ttccctccaa
    11701 cagggaaggc tttgcctctg acggtaaatg gaaagccgac ggatgcccgc ttgcacgtgt
    11761 cgggagacgc agcatggctc gcggtggaag tggacgtcgg accggcagat acggttatcg
    11821 aggctgggta tccctgtgac gggtgcagtg cggatcgtgg tgtaccagcc tcaaaacgcc
    11881 tttacggcat gaccagctcg cgccggggtt tgccttcgat ggtgagattc accttgcctt
    11941 caaggccctg aatccaacgc cggaaacgcc ggaacccgcc gaccggctgg ccgttcacag
    12001 ccgcgatgac gtcgcccgga cgaaaacctg ccttatcggc cgggctgccg gccacaacca
    12061 ccgagaccac cgggcccgtg gccgactccg gcagggcgaa gcgggctcgt gtctccgagt
    12121 tcgcgaccga gaacgtcatc cctcgccagg tgaaatccgg ttcaatgttc acgccccggg
    12181 ccggcgccgg tcgtagcccc ggcacaacat ccaaatccag cgactggccg tctcgcacga
    12241 tcgaaagcgg cactgccttg ccgattcccg acatcgcaac aagacgtacc agctcgtccg
    12301 cattggcaac cggcttgttg tcgaaacgca gaatcgcgtc tccctccctg agcccggccc
    12361 gctgggcggg tgattccggc tctaccgacg agatgacgat tctcgcgctc gcacgaccgc
    12421 ctattcgcgg acgattccga tcctgaagat gaacgccaat gtacccgtat cggacctcct
    12481 gaccgtcgac caactgatcg atgatccccc gagtgtcctt gccgagcgaa atggcatacc
    12541 ccacgccgtc gctgccgccg gaacgcgagg agaccgctgt cgttatgccg accacctcgc
    12601 ccttgagatt gaccagaggg ccgccgctgt tgcccggatt gatccttgcg tccgtttgga
    12661 tcaggttgcc gtagtagcga tcgggatcaa gcttttgagt gagatcctga cccaacgcgc
    12721 tcacgatgcc gaatgacatg gccgcacgac cgtcgccgtc gaaagccgtg cccagcgggt
    12781 tccccatgac gatgacgaaa tgaccctgct gcaacgactc aacgtcaccc cacacggctg
    12841 gcttgaggtt ctcggcgtca atcgacagga ctgccaggtc gctccgcggg tcggtcgtca
    12901 tcccctggac gatgtagcgg tctccggtcg acagaatgca ctcgatcctg tccggatcga
    12961 cgtcatgaac gacgtgctca ttggtcagga tcagcccctg tcggtcgatg atgacgccgc
    13021 tcccgatcga cggcacgagc accccgtgca ccgagcggcc cctgcccgac ggcaaggtcg
    13081 tcacgctcgc atcagcgtgc ggggcggcaa atcggcgcag ggcccgaatc gcaaccacgc
    13141 tcggccgaac ctcctcggcc acggccgcca gcgtgttctg caactgctcc acacgcagca
    13201 gatcctcagc cgatgggccc gcgaggaccg atacccccgg cacaacgccg acgataagca
    13261 taacccacat ggtcaaacga aacatcgccg tcctcagcaa tagccatcaa gtccatcgca
    13321 gcgacgctat tatacccgcg acgcaccgca atgagcgaag taggaccgtc accctcgtcg
    13381 gccatttctg tcccaaaggt gaaccgagag caagacagat tgccccaaag gggcaccatg
    13441 tatgtggtgt ttactccctt tggcgggttg caatatctgc ttctgcccca aaggggcaca
    13501 ggtatgtagc caggggcgca agcccttggg acaggacaga tgacccggac agatcagccc
    13561 cgcagggacg gcagaaggtt cggtccggaa cccctcccgc ttggtccaga atcctttctg
    13621 gaccgctcgg tcgaaaatcc cctcggtcca gaatcctttc cggaccgccc tctgatcgga
    13681 atctatccca cgttgcagac tcattgacaa tgggaatcgt cagaaggatt gccccaaagg
    13741 gccacgggtg tgttgtgttt cctccccttg gcgggtggcg gtatctactt ctgccccaaa
    13801 ggggcacagg tatgtagcca ggggcgcaag cccctgggag ccttcgaccg atcagccgcg
    13861 ccgatcatcg cgcccccacc cttgccacgg cgacaacgat ccgcccgccc ggcgtcaacc
    13921 gccggcgggt tgcggtaacg ggcgcatcgc tgtagcctcg gcgacgtacg cggggctttc
    13981 aagaggcaat gctgcccgcg aggcagactc gcgcaggctg aggcggacag aagaccgacc
    14041 atggatctgc gattgccatt cgtgagaaca gtcggcgtcg ccgtagcggc ctgttatgtg
    14101 ctgacgattg gtgcttgtca tgacgatggc cgccgggtcg agtggatcac aagcgagagt
    14161 gctgagttca ccatcacggt cgcaggcacg gcggaccacc accgtatggg cccgggctgg
    14221 aagagcgacc ccggctcaac ccacaatccc actcggctgc tcattatcaa taaccgcggc
    14281 gatcggtcgg tgatcaaccc gcgcattgtg gtcgatgggg caccggactg gtttgacatt
    14341 catcacattg tatgccagat cgtgcgccca ggcatgaccg atgaggaaaa ggcttttgcc
    14401 ctctggcagt ggattcgccg taatctcagc gacggcccaa cctacggcag ccccctttgg
    14461 ggcgacacgc gcagcatgac ccggttcatg aacgcgtgcg gcacgggggc ttgcggaacc
    14521 taccacatcg tgatgcctgt catcggcaag cacgccggcc tgcgaacctt cagcggctgt
    14581 ttcgccgact gcagccatgc ggttcagaag gagttcttcg acggccagga acgattcctt
    14641 gacgctcaca tcccgcacga ggagggtcag ccgggggggt ggttcgccct caagctcaac
    14701 aatgctgagt tagctggcgt ggacgagatc atggaggatc gccacctgat cgaccgggct
    14761 ggcgctggcc ccggccgcgc gggctacgtc gcctacttcg gccccggttg cgggtttcat
    14821 gaggaaaagc cggcgctcga tccgcgcgac atgggcatga ttcttcgtcc cggagagagc
    14881 atcgcctggc agtggacgat gcacgcacca ccgtggcaga aaggcgatca gacgagcaga
    14941 gcagaccgac atggctcagg ctacgtgaaa ttccgccctc ggctctcgca agagcaggtt
    15001 cgatactgcg cggacacgac ggagaacctt cacttcattg aacgacaggg cgacgaggcc
    15061 gtcgtccccc tcgacgtctc aaaggcggcg accgtagtct atcgtctgta ctgcccgtac
    15121 atgatgacgc gggccgcggt ggagggacga tttgaaaccg gcgacgccgg caccgcgacg
    15181 atcgaataca gcttcgatgg ccgcgattac aggcccgctt ggcgttccac gagcaggggc
    15241 atgcacgaac cgcaggtcga actccccgat gacgatcgtt ttcatgagcc ggtgttcacc
    15301 cacgagctct ggttccggat tactcttgag ggcagacaga catgcttgag tgatctgagg
    15361 ctgcgtggcc atttccaggc ataccgaccg tcattgccca gtctgcacgc aggcagcaat
    15421 cgcatccggt tctcgggccg attcgatcct gatccaggcg ataatcccgc cttggcgaag
    15481 gccgttgtgg ttgattactg gtggcgggat ttgcctgacc tgcccgtccc gaggccgcct
    15541 ccggcaccgg tgcacccggc ccccggcggg cagttcggct tcaacgagac cctgcgatgg
    15601 cggcccgccg aggtggccgg gcaggcggga attgccggtt acgaggtcta cgtttccgct
    15661 cgtcccgacc tggcttggcc ggtcatgccg aatacgcatc gactcatcgg ctcacctgat
    15721 cccgctacgg cactcgtatc cccggatgtc ctccgtcacg gtgcgacata ctactggcgc
    15781 gttcgcgggc gaagtacgga cggcatgtgg ggcgattgga gtccgacgtg gtcgttcgtg
    15841 gcggcggggc ccggtgtccc acgcgatctg aaggtcacat acgaccagcc cacaggagcc
    15901 acatccctta gctggtcgcc gccggctgag ggatcgcccg tggatcatta cgaaatctac
    15961 ggcagcagcg aacacggctt ctcgccgttg cgctcgaacg aggaagcacg tgttcacggc
    16021 ggggtggtcc gacggcccaa gaccctcgtc gaggtgacac gggatagcca ctgggatgcg
    16081 tcggcccgga cggaggtgtt ttatcgcgtc gtggcggtcg acatgcaggg caaccgcagt
    16141 gctcccacgg ccgtcgtcaa agttccttcc ccggcgttgc tcccggtcga tctgccccca
    16201 gccagggttg gtcacgaata ccgcttcaag ctgcccgcgc gactccgcac cggccgcttc
    16261 gctttgtcat tgagaaaagg catcatcacc gatggcgccg acacgccgag attcgcgctg
    16321 gccgatatcg aatcgctcag gatcgatcca tcaacaggtg aattgaatgg taccccaatg
    16381 gtcgtgggaa accacatcct gaaggtccat cttgaggacg gtcggggcgg caaatgcgat
    16441 cgccagtacg cgcttgtcgt gcaaccctag cctgtgggct gtcaaaccgg ctctaatccg
    16501 gtatcatgcc ccttggcttg gggcgaggtt gcctggccat gactggttcg ctttatcaac
    16561 gagagaaagg agatggatat gagggctttc ggaaggatct ctggaaccat ggctgctgca
    16621 ttggccgcgg ttggcttctg gtgtgcccag gcgtccgccc agcaggcgga gaaattcaac
    16681 ggcctccact tgagcttggg caatctgtca cgattgtcgg acgccaagac ccgctcaatt
    16741 tcccctgaga acttcaccgg cgagaagggc aagggcggca tggccaccga tgggaccggc
    16801 aagaacgccg cccgcgaact cggccagggc tggaaaatat cgccctccgt gcgcatcaag
    16861 gccaacacca ctttcacgtt ggccgatatc aagggttcgg gggcgataca gcagatctgg
    16921 atgactccct ccggacattg gcggtttctg atccttcgca tctactggga cggcgaaacc
    16981 gagccgtcgg tcgaggtgcc cgtcggcgat ttcttcgcct gcggatggaa catgtacgcc
    17041 caggtcagct cgttggccgt ttgcgtcaat ccgggcagcg cgttcaactg ctattgggtc
    17101 atgccgtttc gccagtcggc caggctgacc ctcgaaaacc tgagtgacaa ggacgaaacg
    17161 gtctattacc aggtgaatta cacgctgacc agagtgcccg aggacgaagg ctacttccac
    17221 gcccagtttc gacgcaataa cccgctgccc tacaagcagg atcatgtgat cctcgatggt
    17281 gtcaaaggca aaggccacta tgtcgggacc tacatggcct ggcagtcgaa caacgtcggt
    17341 tggtggggcg aaggcgaaat caagttcttc atggacggcg acaaggagtt cccgacgatc
    17401 tgcggcaccg gaacggagga ctacttctgt ggctcctacg gattccacgc caaaaccgcc
    17461 gacggcaaga ccgaataccg cgagtacacg accccgtatg cgggtatgcc tcaggtgatc
    17521 cggcccgacg gcctcaacga gtcccaacag cgattcggct tgtaccgctg gcacatcatg
    17581 gaccccgtcc gcttcgagag cgacctgaaa gtgaccatgc aggccctcgg ctggcgctcc
    17641 ggcggacgat atctgccctt acaggacgac attgcctccg tggcctactg gtatcaaacc
    17701 gagccgcacg cgccgttccc caagctgccg gacaaggacg gcctggaggt caattagtct
    17761 cttccgtcgg gattggtgcg cattttgcgc gcatgtttct tgatcgcaga acgtttgggt
    17821 tgcggcccgc aaaaaggcgg tccgccttgg aggctgttag tccccggctt tcattctact
    17881 gacgacccaa gggtcgagga ggctttcacg tggacaaaca agaacgaatc ggacgacgca
    17941 atgtgctgaa atgctcggct gcggggttgg ccgctctgtc ggccttgtcc ggagccatgt
    18001 ccggctgcaa cgcgaagtcg gaaagaccgg ctgtggctcc caagcaacaa agccggacgc
    18061 cacccggcga acgggtcgtg gtgggtgtca tcggtacggg cggacttggg ttgggacacc
    18121 acattcgggg ttgtctcctg cccaatgata acttcgaggt tgcggcggtc tgcgacgtcg
    18181 accgcaacca ccgcgatgcc gcggccagga tggtaatgga aaaaaccggc aagcgggtcg
    18241 ctgcttacga agattttcgg cacctcatcg atcgcaagga catcgatgcc gtgctgatcg
    18301 ccacgccgga tcattggcac accatccccg cgatcatggc cgctgaagcg ggcaaggaca
    18361 tttattgtga gaagccgttg accctgaccg tcaacgaagg aaaggccttg gtcgccgccg
    18421 tgcgccgata cggtgtggtc tttcagacgg gcagccaaca gcggtcggac aacgccttcc
    18481 gaaaggcctg tgagctggtc cgcagcgggg cgattggcaa gctcaagaga atcgacaccg
    18541 ttctccatcc tgttcccaag gggcagtggc aaccggtaac cacccctccg cccagcctca
    18601 actggaactt ctggctcggc caggccccat atgtcgacta ctcgcccaac cgcgtccatt
    18661 accagttccg ctggttttac gactacagtg gtggtgtcat gactgactgg ggtgctcatc
    18721 acaacgatat cgcccagtgg gccaacggca cggacggcac cgggccggtt tgggtcgacg
    18781 gaacccgcgc tacctttgct gaaagcggta tgcacaccgt tcccttgacc ttcgacgttc
    18841 actacaagta cgccaacggc gttgagctct ggtgccacac caatgaccag acctacgacg
    18901 acggaacgaa gttcggcaac ggcgtgaagt tcaccggcga aaacgggtgg atctttgttg
    18961 ctcggggcaa tcgccttcag gcctcggatc cggacatcct ggacgtcgag cccgccgtca
    19021 aactctacaa cagcagcaac catcaccgga attggctcga ctgtatcagg agccggcagc
    19081 gctgcatctg cgacgtggaa atcgggcatc gttcggtgtc gatctgccac atcggcaaca
    19141 tctcgatgcg gctcggccgg gcgctcaact gggaccccgc gaccgagcag ttcgtcgcgg
    19201 acgaggccgc aaaccggatg ctcagccggc cgatgcgcgc gccgtggcac ctgtgaccag
    19261 ggtgccaccg ggccgcccgc agagggcccc ctgggatgcc ctgggcggtt caattgccgg
    19321 tgccaggatg gaagatggat gtaacaggct ttgaatagat gtgaccgcgt aagcggagga
    19381 gtcacaagat ggcatcgatt agggagttaa ttgtgcaatt cagcggtgag caggaggccg
    19441 catacaaagc atatcgacag gtggaactga tggtcgccaa ggccaatgca ccgggcagcg
    19501 aggccgagcg gacccaactc gcggcggaac tgaccgagca actgtgggcc ctgtccgagg
    19561 ccaagaaaga cgacaagggc aatgatctgc cgcgaacgcc cacccatccc gtggcaacga
    19621 ggatcaagat cctgcgtctg cttcagggca tggccggcga taaggaagta ccggcgatcg
    19681 ccaaggcgct cgacgaactc gaactccggg aggatgcccg ctgcacgctg gaccgcattt
    19741 cgtcacacga gtccgacaag gcgctcgtca acgccttgga cgccgccgtt ggggcggaat
    19801 tccgagtggg tatcgtgtct tccctggccg ctcgaccgaa cgcttatgtg atggccgcct
    19861 tgcagaaagc aacggccgat tgcgatccgg aagtcgcctt ggccgccgtc gaagcactgg
    19921 ccaacttccc cgaaccggcc aacgacgagt tcattgccaa cgccgcccgg tgctcctgcc
    19981 cggtcgcacg tcgccgggca ctcagcgccc gtgtgcgatt cgccgagaac
//
LOCUS       DAOTPW010000095        19785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2811_length_19785_cov_4.812570, whole genome shotgun sequence.
ACCESSION   DAOTPW010000095 DAOTPW010000000
VERSION     DAOTPW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19785
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2811_length_19785_cov_4.812570"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..877)
                     /gene="nusG"
                     /locus_tag="P5316_10275"
     CDS             complement(200..877)
                     /gene="nusG"
                     /locus_tag="P5316_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRR85387.1"
                     /translation="MSGPETDNKDASQAAGGGGGTPDHVSPASESTTAVKGVEPTRPG
                     MKWYVLRVASNKEDQVCETLERKVRIENLQNRIGRILVPTAKEKRMKGGQVKIIEKKL
                     YPGYVFVEMATEADGTIPENIWFLVKETTGVGDFIGSDGKPTSMKDHDVEMMLLAAAK
                     PDDEASLQGLEFAQGDKVKIKEGSFQNFEGTVESIDETKGIVTVLLTIFGRSTPVDVE
                     YWQLEKI"
     gene            complement(874..1401)
                     /gene="secE"
                     /locus_tag="P5316_10280"
     CDS             complement(874..1401)
                     /gene="secE"
                     /locus_tag="P5316_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRR85388.1"
                     /translation="MSDEVVRQESREEQAQQRPTIERPAGGGFALRLYKPGQGYYTRI
                     GTAIGIGILAVWGAYALMNQIENSGLSGPYRLPIQYGVPAGFIIAMGVLVYWLTGLSR
                     KANDFFIATEGEMKKVRWSTRKELTRSTKVVIFTVVVLGVFLFVWDVFFMVFFQAIGV
                     LKGAPALSRLFGSGS"
     gene            complement(1512..1585)
                     /locus_tag="P5316_10285"
     tRNA            complement(1512..1585)
                     /locus_tag="P5316_10285"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Trp,seq:cca)
     gene            complement(1678..1845)
                     /gene="rpmG"
                     /locus_tag="P5316_10290"
     CDS             complement(1678..1845)
                     /gene="rpmG"
                     /locus_tag="P5316_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRR85389.1"
                     /translation="MAKAVKREYVWLQCSECGDLNYRTSVNVQGGTPKLELKKYCPRS
                     RKRTVHKVKRK"
     gene            complement(1961..3166)
                     /gene="tuf"
                     /locus_tag="P5316_10295"
     CDS             complement(1961..3166)
                     /gene="tuf"
                     /locus_tag="P5316_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRR85390.1"
                     /translation="MAKGVFSRTKPHVNVGTIGHVDHGKTTLTAAMTAVQAKKGLATF
                     KSYDEVAKASEKEGRRDASKILTIATSHVEYETEKRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAADGPMPQTREHILLARQVNVPAVVVFLNKVDLVDDAELLELVELEVR
                     ELLSKYDFPGDETPIIKGSASEALFNPDNPEKTKCIQELLDAIDSFIPEPVRDVDQPF
                     QMPVEDVFSIKGRGTVGTGRIERGKVKVGDQVEIVGLGETRKTTVTGVEMFNKTLEYG
                     QAGDNVGLLLRGVEKDELERGHVLAAPGTITPHTKFEAQVYVLTKEEGGRHSPFFSGY
                     RPQFYFRTTDVTGGLTLLGGAEMCMPGDNVLLKVELGKPIAMEEGLRFAVREGGRTVG
                     SGVVTKILE"
     gene            complement(3406..3477)
                     /locus_tag="P5316_10300"
     tRNA            complement(3406..3477)
                     /locus_tag="P5316_10300"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3443..3445),aa:Thr,seq:ggt)
     gene            complement(4079..5746)
                     /locus_tag="P5316_10305"
     CDS             complement(4079..5746)
                     /locus_tag="P5316_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRR85391.1"
                     /translation="MFQLLVVVATILGACAVTDGDQYLYEKMTPEQLKQAVVECPVAY
                     LPTGIPEWHGEQNACGLDALKAETLGQMAAKMLGGICFPTVWTGPGGSTPFDPRLYPR
                     GTVTIDEKLYQRQMEELLNHLESLGFKVAVHLSGHYPGVVPKLAEKFNQRGRMKVISA
                     SENQVVRGMPAGDHAAAWETSLLMVLRPGLVDLTRLPPLPEGVKPAGEKVAEPYQFIP
                     TKEYYGVYGADPRVWANKTYGRRGTEAILEGLAREVAEALGDSSYGQKRTGIEWPGDS
                     GEGSGVRYCHQLPYQWCERFDKAPIVYWPLVAAAESPQSRQSIMRGAEQLAVERGGMV
                     FPAFSYAPSTDKGCVAVSAESFRQIVTEVVETLTGMGFRIVVLMPGRDVEPGSREALR
                     AVKLPEEQGRVLVGDPGSGAAPRGLAEAIIATIPRQPSNVRLGGPWTIDGELQVGALA
                     EQVSASPGGERIYETTFELSADQARMSAMLDLGQVVNLCEVVVNDSSPQVDHWPPYRL
                     LVTGRLKPGVNSLKIVARNKPQPTLDKFFHTPGPPVLAGPVILRLWR"
     gene            complement(5891..6547)
                     /locus_tag="P5316_10310"
     CDS             complement(5891..6547)
                     /locus_tag="P5316_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85392.1"
                     /translation="MAVVVEDYLGEKISAFAFDDQLTELSAKTKDETVKRIAHELWFH
                     YDDCKDHKVVLSGAQWDYFQRLVLILKSDASVRVERRRTWSWRQLVAAMLLVCFAAVL
                     ASLGWGLHLLVVSVPFGLASMVLSRCGRTMPVDQTNHLIRLVPFASLNQILAVRRKTT
                     GFKKSPYPPALIDRRIRSPFMDRVLAWQWRVMWLLFSPIALLGQCFPDTEQRIEVAVD
                     "
     gene            complement(6689..7798)
                     /locus_tag="P5316_10315"
     CDS             complement(6689..7798)
                     /locus_tag="P5316_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRR85393.1"
                     /translation="MFILALMDAGRSESKPGIEQNAEAEPSLVRRILFPSWRQRKRAL
                     RREAWLLVIGSYAAVVFAFLWPQDFRETSQAYVAVSLIAFMVRVFEFHLGLLLVIVAV
                     VSLHTRQWRLFAATIPLFLWTLGPAVWTYRPKTRPSLVGEVVTVASVNLLMVNRNSAP
                     LVEEIRASGADIVFLQEYTDHWHEVLRASLGQEYPYECHVCREDSFGAAIYSSRPFIS
                     EPELDMPLGVSSLPQIRAVVEISHRPVAIYNVHLLPPIGLEYTTETRLQFADLLAHLA
                     GESRPFILAGDFNFTERTPQAAALRRLGLIEAFDQGGWGRGSTWPVNSVFRYVPGLRL
                     DHIYLGGGLACSDCRVGQGRGSDHRPVIAKIGFSW"
     gene            7990..8178
                     /locus_tag="P5316_10320"
     CDS             7990..8178
                     /locus_tag="P5316_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85394.1"
                     /translation="MANVKEQLRELLDRLPDDCSIEDVQYQLYVMETIQRRIELAEKG
                     EVVSQADAEKRLDKWITK"
     gene            8163..8471
                     /locus_tag="P5316_10325"
     CDS             8163..8471
                     /locus_tag="P5316_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRR85395.1"
                     /translation="MDHEVVWLKAALDDLDDIAEYVAADSPAYASVVVSKMLAQAREL
                     AGFPQLGRCVPEWNRPDVRERIVYGYRLIYRIVGDLVIILAVIHGSRRLLDNLQDRPH
                     "
     gene            complement(8788..9948)
                     /locus_tag="P5316_10330"
     CDS             complement(8788..9948)
                     /locus_tag="P5316_10330"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRR85396.1"
                     /translation="MDKPDKKPSTPNFSSGPTCKRPGWSVHALTGALTGRSHRSKPGK
                     ARIQQALELTREVAAIPADYLIGIIPGSDTGAFECAMWSLLGARPVTAMAWESFGEGW
                     VTDINKQLKLNAKVMKAPYGEIPNLAEVDWDTDVVFTWNGTTSGVKMPDDAAPPVDRK
                     GLALCDATSAVFAMPVPWERLDAITWSWQKCLGGEAAHGMLVLSPRAVERLESYSPPW
                     PMPKIFRLTKGGKLSAGIFQGDTINTPSMLCVEDYIDGLNFARNLSFGGRTGLQACFA
                     KSQANLEAVAEFVGRHDWIDFLPVKPEIRSCTSICLKITAEWFRKLSPEDQAAWCKKV
                     VSALDSEGVAKDFGAYRDAPPGFRFWGGPTVEPADMAIALEWLAWAYDANKK"
     gene            complement(10223..10897)
                     /locus_tag="P5316_10335"
     CDS             complement(10223..10897)
                     /locus_tag="P5316_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006884274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRR85397.1"
                     /translation="MSAIRVTVWNECWHEYNTEVVPKIYPDGIHGAIAAGLREESGLE
                     VRTATLRDRECGLTDDVLAVTDVLVWWGHMRHHEVPDAVVERVLGRVWNGMGFVALHS
                     AHYSKPFQRLMGTPCGLKWRVDNKKEILWVVDPTHPIAGGIDEKIELPQTEMYGEWFQ
                     IPAPDELVFISWFEGGEVFRSGCCWKRGKGRVFYFRPGHETLPIYHNPDVRRVLRNAV
                     RWAAAK"
     gene            complement(11103..11966)
                     /locus_tag="P5316_10340"
     CDS             complement(11103..11966)
                     /locus_tag="P5316_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR85398.1"
                     /translation="MQHYLKQIDEAPLLTAKEEIELGSIIQKSIRAGELLREGRISLQ
                     EKEKLEEEAARARDRMVLSNLRLVVNIAKNYLNRGLSLSDLIEEGNIGLLRAVEGYDP
                     TMETRFSTYASWWIKQAIKRALINSSQPVHIPAYMVAMIADWKKAWAELENQLGRHPS
                     LQEMAEHMKLSERKLRIIRRAVKAFSAPVQPGGAEGSFGLSDILADSRTPSPEAAAFS
                     NADAEVIQRLLEKIDEREATILRMRYGMGEHQAPMTLKDIGAKIGLTRERVRQLEREA
                     LKKLREAFNEM"
     gene            complement(12221..14044)
                     /locus_tag="P5316_10345"
                     /pseudo
     CDS             complement(12221..14044)
                     /locus_tag="P5316_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245712.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            14395..15114
                     /locus_tag="P5316_10350"
     CDS             14395..15114
                     /locus_tag="P5316_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85399.1"
                     /translation="MAGRPGRLKWVRRILCAAGLLILVTGVTLWLMFQHIPSWYRPPA
                     IAPANHQRIRNDWAGSRDSLQQAMLREEPFEFAVTQDQINSWLAIREAIWQLSREWLP
                     SALSEPFVRIEKDSLRLAATYRSGSIRTVLSARLEIALRADGIWVRLAGLDAGSLGVP
                     DAWVRERLRLIDGRAWSPGKKSRYQLGGPPLPSLPTLPDGAVFPATWVWKESELALPF
                     EIAGLRFEPGVLVVSLRPLPR"
     gene            complement(15111..15488)
                     /locus_tag="P5316_10355"
     CDS             complement(15111..15488)
                     /locus_tag="P5316_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HRR85400.1"
                     /translation="MGEPGAAFVLLRHDTRDGVHWDLMLDTGSVLATWQLAADPSAAG
                     PGPQEISARRLPDHRYDYLDYEGPISRNRGYVTRSDRGTYRILEDNSRSYVVILSGER
                     LSGRFRLQADGPSGESWRFTRLD"
     gene            15594..16190
                     /locus_tag="P5316_10360"
     CDS             15594..16190
                     /locus_tag="P5316_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85401.1"
                     /translation="MMRCTSPVSMVTVAVGLSLLAVMTGCTPDVTDIRNVGIKQYQAG
                     LQIESMATFRRALELAPNDAVSNYYMGLHYKNRADRKFSEGDLTGAYKQLDTAIMYFN
                     QATTSIPSFIEAIKEKKDALARRGKYEQAVNMAEHVAQRIPGDKVTQYVILGNLYRDA
                     GDADNALRAYKQALAMDPSSTEARVEIERLYERARRPR"
     gene            complement(16246..17250)
                     /locus_tag="P5316_10365"
     CDS             complement(16246..17250)
                     /locus_tag="P5316_10365"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HRR85402.1"
                     /translation="MDKQQATGPGELAYQLSALWQRQTETWEMLAEGLAALRQARTRT
                     FHVNGWRVTAQCNPARIKSSGAKVDAESLAQRPCFLCTENRPVAQASVPYRDRWLILC
                     NPAPLFEPHYTVAWIDHEPQRVGPAIEGLLSMSRDLDGRYTVFYNGPASGASAPDHLH
                     LQAARAGALPFETQLATQLAAGDPADGQRRLEWVRDDPVRIAVTRPGSLPVIVLMGDS
                     KDAMAASLREVVIVLGEVRPVEPEPMLNLFATYTEERWVTWLFPRGAHRPRFYGTGED
                     DFLISPGAADLAGIVVSPRPRDFERLTDEIMQTIFREALLPPESFAEVRDLLARKGRR
                     "
     gene            complement(17380..18513)
                     /gene="proB"
                     /locus_tag="P5316_10370"
     CDS             complement(17380..18513)
                     /gene="proB"
                     /locus_tag="P5316_10370"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRR85403.1"
                     /translation="MPSTTLRKQIMAGVRSIVVKAGTNLLTCENGKLDQTVIASLTDQ
                     IATLIERGVKVTLVSSGAVGAGMGKTGVARRPRSMPVLQAVAAIGQPSLMSMYEREFA
                     RHELLVGQVLVTRKDFEQRNRYVNISNTISALQRLKAVPIINENDTVAIDELDRFADN
                     DTIAAMVTNLLRADLLVLLTVVDGLLDSGGQLVDLVPAVNTEVKSLARAERSPLGSGG
                     MFSKLGAARMVSEAGECVVIANGREPGALLRLLNGERVGTIFAPAARKLSARQRWIVG
                     AARPTGVIVVDAGAANAVHTQGKSLLARGVTRIEGKFDRGSIVQIAGPDGRVLAHGIS
                     NYSSTELAMIKGLKSCEIAAALGSKPFDEAVHRDNLVIIAGQR"
     gene            complement(18491..>19785)
                     /locus_tag="P5316_10375"
     CDS             complement(18491..>19785)
                     /locus_tag="P5316_10375"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRR85404.1"
                     /translation="MNSIRGLVEMWRARCPELDFVISATTDTGIDQARKLFKDLIVVR
                     YPLDFSRFVRRALDRIAPTMIVLVEAEVWYQFISQAKVRNIPVVVINGRLTEQKSMRR
                     FRWIMPIARRMFGSLTWVGALAEEHADRFRRLGVPADRVTVTGSMKWDTAQVADSLPG
                     RDELARAMGIDRNRPVWVCGSTGDGEEAIILRAYALLRHRRPQLQLMIVPRKPERFDE
                     VAELIRQSGFACLRRSRCPDGDRPAGEVCEKGRARSEPPEAAETCRPALDHAAGLGRR
                     SDLDAALAHVPGGSSGEESPAVRLGDTMGELRKFYALADVVFVGRTLADMGGSDMMEV
                     AGLGRPIIVGPHTENFAEAVERLDAGRAIRILSVDSSAPDAAERLAEAVSDLLDDPVA
                     AGEMARRGQEIVKRNRGATERTVNHLMEIMERAQHDAS"
BASE COUNT         3888 a         6618 c         5507 g         3772 t
ORIGIN      
        1 ggcgtcgcct ttccccccgg ggcctgcagt ttgatcttga caagaacttc cttggccacg
       61 ttatcaacct ctccagcggt tcagatcccc accgaccgga ccccactgtg tcatccgacc
      121 gtctaaggcg gaccgcaacc caagcctact gccatccaga aacatgtgcg caagacgcac
      181 aatgtctggc cgcaaggttc taaatcttct ccaactgcca gtactcgacg tccaccggcg
      241 ttgaccgccc gaaaatcgtc agcagcaccg tcacgatgcc cttggtctcg tcgatgctct
      301 cgaccgtgcc ctcaaagttc tggaacgagc cttccttgat cttaaccttg tcgccctggg
      361 cgaattcgag accctgcagc gaagcctcat catccggctt ggccgccgcc agcagcatca
      421 tctcgacgtc gtggtccttc atgctcgtcg gcttgccgtc cgagccgata aaatcgccga
      481 ccccagtcgt ctctttaacc aggaaccaga tgttctcagg aatggttccg tccgcttccg
      541 tcgccatctc gacgaacacg tagccggggt agagcttctt ctcgatgatc ttcacctggc
      601 cgcccttcat ccgcttttcc ttggcggtgg gtaccaggat tcggccgatg cggttctgca
      661 gattctcgat ccgcactttg cgctcgaggg tttcgcagac ctgatcctcc ttgttgctgg
      721 ccacgcgcag gacgtaccac ttcatgccgg gtcgggtcgg ctcgacaccc ttgaccgcag
      781 tcgtggactc gcttgccggc gagacatgat ccggcgtgcc tccgcctccg cctgcggcct
      841 gacttgcgtc cttgttgtcc gtctccggac cgctcatgat ccactcccga acaggcggct
      901 cagcgcgggc gctcccttaa gcacaccgat ggcctggaaa aacaccatga agaacacgtc
      961 ccacacaaac aggaaaaccc cgagcactac taccgtgaaa atcacaacct tcgtcgaacg
     1021 ggtgagctcc ttgcgggtcg accagcgcac tttcttcatt tcgccctcgg tggcaatgaa
     1081 aaagtcgttg gccttcctcg acagccccgt cagccagtat accaggacgc ccatcgcaat
     1141 aatgaagccg gccggcacac cgtattgaat gggcagcctg tagggcccgc tcagcccgga
     1201 attctcgatc tggttcatca gggcataagc cccccatacc gcaagaatac cgatgccaat
     1261 cgccgtaccg atgcgggtgt agtatccctg cccgggcttg tacagtcgca gggcaaagcc
     1321 gccccccgcc ggtcgttcaa tcgtcgggcg ctgttgagcc tgttcctccc gcgattcctg
     1381 tcttacaact tcgtcgctca ccggggactc ctcttgcccg cgctaccagt tagtcgatcc
     1441 actcgccatc ctgcttcgtt ctcgccccgc gccccgagtc gcaaccaccc gatcacccga
     1501 cccttgacgc acaggagtgg agggactcga acccccaacc gccggttttg gaaaccggtg
     1561 ctctagccaa ttgagctaca ctccttggca gaccggccga aaggtcccga aacgtcagcg
     1621 gtccccattt cccggcacgg ctcgggcagc cgcgaagaca accggctgcc cacgccgtta
     1681 cttccgtttc accttgtgca ccgtccgctt acggctccgc gggcagtact tcttcagctc
     1741 cagcttggga gtgcccccct gcacgtttac cgacgtgcgg tagttcaggt caccgcactc
     1801 tgaacactga agccagacat actctcgctt tactgccttc gccatagcgc ctcatcccag
     1861 agacaacggc gaaggccggt tgccaccggc ctccgcccaa caactgtcga tccatcacac
     1921 cgcttgcccc cggaacgccc caggcggcga aacatcggtg ctattcgaga atcttggtca
     1981 ccacgcccga tccgacggtt cgaccaccct cgcgaacggc aaaccgcaga ccttcttcca
     2041 tggcgatcgg cttgcccaac tcgaccttca gaagaacgtt gtcgccgggc atgcacattt
     2101 cagccccgcc cagcagcgtc agaccgccgg tcacgtccgt cgtccggaag tagaactgcg
     2161 gacggtagcc gctgaagaac ggcgagtggc ggccgccttc ttccttcgtc aggacgtaca
     2221 cctgagcctc gaacttggta tgcggcgtga tggtgccagg ggcggccagc acgtggcctc
     2281 gctcaagctc atccttttca acaccacgca gcaacaagcc gacgttgtcc cctgcttgcc
     2341 cgtactccag cgtcttgttg aacatctcca cgccggtgac ggtcgtcttc cgggtctcgc
     2401 cgagaccgac gatctcgacc tggtcgccga ccttgacctt gccgcgctcg atacggccgg
     2461 tgccgaccgt gccgcgcccc ttgatgctga acacgtcctc aacgggcatc tggaacggct
     2521 ggtcaacgtc acggacgggc tccggaatga acgagtcaat cgcgtccagc agctcctgga
     2581 tgcacttggt cttctcggga ttatccgggt tgaacagggc ctcgctggcc gagcctttga
     2641 taatcggcgt ctcatcaccc gggaaatcat acttgctcag caactcgcgg acttccagct
     2701 ccaccagctc gagcaactcg gcgtcgtcaa ccagatcgac cttgttcagg aagaccacca
     2761 cggccggcac gttcacctgg cgggccaaca gaatgtgctc gcgggtctgg ggcattggtc
     2821 cgtcagccgc cgacaccacc agaatggccc cgtccatctg ggccgcgccc gtgatcatgt
     2881 tcttgacgta gtcggcgtgt cccgggcagt ccacgtgggc gtagtgtcgc ttctcggtct
     2941 cgtattcgac gtgcgaagtg gcgatggtca agatcttgct ggcgtctcga cggccttcct
     3001 tttcggacgc cttggcaact tcgtcgtagc tcttgaaggt tgccagtccc ttctttgcct
     3061 gcacggccgt catcgccgcc gtcaaggtcg tcttgccgtg gtcgacgtgc ccgatggtcc
     3121 caacgttcac gtgcggtttc gtgcgtgaga aaacgccttt ggccatgaat caactccttc
     3181 agttctgcaa tcgtatccgg cgcctatcgc gtcgaatgaa ccggcaacca ggcaacgatc
     3241 cgacaggccg ttcctgtctt tgccccaaaa atacttccca acgtctatta ccgtcaccga
     3301 tccgcggatg catgcggaac aaagagaatg catcactttg cccgccaagc cctcacgcat
     3361 ccgccgggag gcgtcccatc ccgtgccgcc ctccgtctgg cataaagctg ctgatggggg
     3421 ttgaacccat gacctcgtcc ttaccaagga cgcgctctac cactgagcta cagcagcggg
     3481 cgtctctcgg ttcgtcacgc gtcgcagccc acgccaggca gacccacaac ccggacaaac
     3541 cgcgggcata ccgtctctgg tatgcactag gtgccggagt ccgacaagta tacggcttcc
     3601 gcagcttctg tcaaacaagc aacccgctcc ctccacgcgg tcttcgggga aggctttgcc
     3661 cgctccaagc cggtccatcg gaccccgaaa gcatgaggca tcgcagtaac ctattgtcaa
     3721 aaaagatttt atgtctaata agaccgttcc gctaggactg gaaaaacgct aactgcaacc
     3781 tcctgacgac ttatcggaca agaacagtgc tcaacaaaag cgccggaatc agtctgcggc
     3841 tgaccacctg atggattgaa caaaggcgag ccgcctccag cgctgacctg cggccttaac
     3901 cacccggtca cagcatcgcc ccgaccgcag tcggctcaga acaatacggg ccgatcaacc
     3961 gcatcaccat tgttcagaac ctccaatgtt ctctgcgaca ttgcccaatc cttgcgccga
     4021 gtgcgccaga ttcgcaggat acggcctggc agcaagtatc gtagggaaag tgacggcgtc
     4081 atctccacaa ccgcaaaatc accggcccgg ccaagaccgg cggacctggc gtgtggaaga
     4141 acttgtccag cgtaggctgt ggcttgttgc gcgcaacgat cttgaggctg ttgacccccg
     4201 gcttcaatcg gcctgtcacg aggagcctgt acggcggcca gtgatcgacc tgcggcgagg
     4261 aatcgttgac caccacttca cagagattga cgacttggcc taaatccagc attgcggaca
     4321 ttcgcgcctg atcggcagac aactcaaacg tggtctcgta aatccgttca cccccgggag
     4381 aagccgatac ctgctcggca agagcaccaa cctgcaactc accatcgatc gtccatgggc
     4441 cgccaagccg cacgttgctc ggctgccgtg gtatcgttgc gatgatcgct tcggcaagcc
     4501 cccgaggtgc cgccccgctg ccgggatctc cgaccaacac cctgccctgc tcctcaggca
     4561 gcttcacagc cctcaaggcc tcccgtgaac ccggctcgac gtcgcgaccg ggcatcagga
     4621 caacgatgcg aaaacccatg ccggtcaggg tctccacgac ctccgtcacg atctgccgga
     4681 acgactcggc cgataccgcc acgcatcctt tgtccgtcga tggtgcgtac gagaacgccg
     4741 gaaagaccat tccgcccctc tctacggcca gttgttccgc cccccgcatg atcgactgcc
     4801 ttgactgagg tgactcggcc gcggcaacaa gcggccagta gacgatggga gctttgtcga
     4861 agcgctcaca ccactgatac ggcaactgat ggcagtatcg aaccccactc ccctcccctg
     4921 aatcgccggg ccactcgatt cccgtccgct tctgcccgta agagctatct cccagggcct
     4981 cagccacctc acgagcaaga ccttccagga tggcctccgt tcccctacgt ccgtaagtct
     5041 tgttggccca gacgcgcgga tcggcgccgt aaacgccgta atactccttt gtcggtatga
     5101 actggtaagg ctccgccacc ttctccccgg ccggtttgac gccctccggc agcggcggca
     5161 ggcgagtgag gtctaccagt cccggcctca gtaccatgag cagtgacgtt tcccacgccg
     5221 ccgcgtgatc accggcgggc atgcctcgga ccacctgatt ctcgctggcc gagatgacct
     5281 tcatcctgcc tcgttgattg aacttctcgg ccagcttggg aaccacgccc ggatagtgcc
     5341 ccgacaaatg caccgcgact ttgaaaccca gactctcgag atggttgagc agctcctcca
     5401 tctgcctctg gtagagcttc tcatcgatgg tcaccgtccc tcgcggataa agtcgcgggt
     5461 caaacggcgt cgagccgccc ggacctgtcc acaccgtcgg aaaacagatt ccaccgagca
     5521 tcttcgcggc catctgaccg agcgtctcgg ccttgagtgc atccagccca caggcattct
     5581 gctcgccgtg ccactcggga atccccgtcg gcagatacgc gaccggacac tcgaccacgg
     5641 cctgcttcaa ctgttccgga gtcatcttct cataaaggta ttgatcgccg tccgtcaccg
     5701 cacatgctcc cagaatcgtc gcaacgacca cgagcaattg aaacatgatt gctacctccc
     5761 gcacgctcaa gcccgacagc ataccagtca ggtgccaagg tgcccagccc ggcgtcctgc
     5821 gaccgattgc atcttcatca cgcctttggc agggtggttc ccgcaggcgt acccgcaagc
     5881 aacccatgcg ttagtcaacc gcaacctcga tgcgttgttc ggtatcagga aagcactggc
     5941 caagaagcgc gatcggcgag aaaagcaacc acatgacccg ccactgccag gcgagaaccc
     6001 gatccataaa cgggctgcgg attctcctgt cgatcagagc cggtgggtat ggagacttct
     6061 tgaagcccgt cgttttccgg cgaaccgcaa ggatctgatt gagagaagcg aacggaacca
     6121 gcctgatcag atgattcgtc tgatcgacgg gcatcgtcct gccacaccgc gagagcacca
     6181 tcgaggcgag tccaaacgga accgagacga ccaggagatg cagaccccat ccaagcgaag
     6241 ccaagacggc ggcgaaacag accaacagca tcgcggctac caactgtctc cacgaccacg
     6301 tacgtcgccg ttcaacgcga acagacgcat ctgatttcag gatcagcacc aggcgttgaa
     6361 agtaatccca ctgcgctccg gagagaacaa ccttgtggtc tttgcagtcg tcatagtgaa
     6421 accagagctc atgtgcgatt cgtttcacag tctcgtcttt ggtcttggca gataactcgg
     6481 tgagctgatc gtcgaatgcg aaagccgaga tcttctcgcc caggtagtcc tcgacgacca
     6541 ctgccattct atctcgtgtg tcgcggtcga tcatcatgac ctccgttccg tgtgagacag
     6601 atatcacacg cagaagtgca agtccagacg tgaaagccgc aggtatgtgt tcgaaacaca
     6661 gactaatcgg ccgcgttcgg agcacgccct accatgagaa accgatcttc gcgatcacgg
     6721 ggcggtgatc ggacccccgg ccctggccga cgcggcaatc cgagcacgcc aggccgccgc
     6781 ccaggtagat gtggtcaagc cgcagacccg ggacatatcg aaagactgag ttcaccggcc
     6841 aagtcgaccc tcgcccccag cccccctgat cgaacgcttc aatcagcccc aatcgccgca
     6901 gagccgccgc ctgcggggtg cgctcggtga agttgaaatc tcccgcaaga atgaatggtc
     6961 gcgactctcc cgccagatgg gcgagcagat cggcgaactg cagtcgagtc tcggtcgtgt
     7021 actccagccc gatcggcggc aacagatgga cgttgtagat ggccaccggc cgatgggaga
     7081 tttcgacaac tgcccgaatc tgcggcagag aggatactcc cagcggcatg tccagttccg
     7141 gctcgctgat gaacggccga ctggagtaga tcgccgcgcc aaacgagtcc tcacgacaga
     7201 catgacactc gtaagggtat tcttgcccca gcgatgctcg cagcacttcg tgccagtggt
     7261 ccgtgtactc ctgcaggaac acgatgtctg cgccggaagc tcggatttcc tcgaccagcg
     7321 gggcgctgtt cctgttgacc atgaggaggt tgacgctcgc gaccgtgacc acctcgccga
     7381 ccagagatgg ccgagtcttg ggacgatacg tccacaccgc cggccccagt gtccacagaa
     7441 acaaaggaat cgtcgcggca aacagccgcc actggcgggt gtgcagcgag acgacggcca
     7501 caatcaccag caacaggccc agatgaaact cgaacacccg gaccatgaac gcgatcagcg
     7561 agaccgccac atacgcctgc gaggtctcgc ggaaatcctg cggccaaaga aacgcgaaga
     7621 ccacggcagc gtacgacccg atcaccaaca gccacgcctc gcggcgaagg gcacgcttac
     7681 gttgccgcca ggaaggaaaa agaatacgac ggaccagcga cggctccgcc tcggcgtttt
     7741 gctcaatccc aggcttgctc tccgaccgtc cggcatccat gagggcaagg ataaacaaga
     7801 cggactgccg aaggcaactg cccgtgaggc cacggtaacg gcaagccccg ccaaacctcg
     7861 gcctgccgtg cctccctgca aatgattgtc ttggaggagg cgtattggta ggatgatcag
     7921 cagagatttg tacgatacga gcgttgcgtt ggcgtgcaaa acgatgaccg ccacgacggg
     7981 ggcccttcca tggcaaacgt gaaagaacag ctccgagaac tgctggatcg cctgcccgat
     8041 gactgttcaa tcgaggacgt ccagtaccag ctctacgtga tggagaccat tcaaagacga
     8101 atcgagctgg ccgagaaagg cgaggttgtc agccaggctg acgccgagaa gaggctcgac
     8161 aagtggatca cgaagtagtc tggctcaaag cggcccttga cgatcttgat gatatcgccg
     8221 agtacgttgc tgcggactca cccgcatacg cctccgtggt cgtgtccaag atgcttgctc
     8281 aagcaaggga attggctggc ttcccgcaac tgggccgttg tgttcccgag tggaacaggc
     8341 cggacgtacg cgaacgtatc gtgtacggat acaggctcat ctaccgaatc gtcggcgacc
     8401 tggtcatcat tctggcggtg attcacggtt cgcgccgtct gttagacaat ctgcaggacc
     8461 gacctcatta gtccaaacgg cgcacgaaca gaaccgcgga gcacgatgcg cacatcctgg
     8521 aacggcggag gccacctgca gcaacaaaga aaaggacacc ggcctccggg gaacgactcc
     8581 ccgaaggccg gcaatgcata tcagaacgac tcgcacaacc gaacagtcca gggcggaccg
     8641 cctttttgcg ggccgcaacc caaacgttct gcgatccaga aacatgcgca agccgcacac
     8701 atatccggcc gtaaggttct aatggtcgaa caggccaata gactaacaga ccaacagacc
     8761 aacataccaa cataccaacg gactgctcta cttcttgttc gcgtcgtagg cccaggccag
     8821 ccactcgagg gcgatggcca tgtcggccgg ctcaacggtt gggccgcccc agaagcggaa
     8881 gcccggaggc gcatcccggt aggcaccgaa atccttggcg accccttcgc tgtcgagcgc
     8941 cgagaccacc ttcttgcacc acgcggcctg atcctcggga ctgagcttcc tgaaccactc
     9001 ggccgtgatc ttgaggcaaa tcgaggtaca gcttcggatc tcgggcttga ccggcaggaa
     9061 gtcaatccag tcatgcctgc ccacgaattc tgcgaccgcc tccagattgg cctggctctt
     9121 ggcaaagcag gcctgcaagc cggtcctacc tccgaagctg aggttcctgg caaagttgag
     9181 accgtcaatg tagtcctcga cacagagcat cgacggagtg ttgatcgtgt cgccctggaa
     9241 aatgccggcg ctcagtttgc cgcccttggt gaggcggaaa atcttgggca tcggccacgg
     9301 gggcgaatag gactccagcc gttcgaccgc tcggggagag agtacgagca tgccgtgggc
     9361 cgcctccccg ccgaggcatt tctgccacga ccaggtgatc gcatccagcc gctcccaagg
     9421 tacgggcatc gcgaagaccg cgctggtggc gtcacatagg gccaggccct tgcgatcgac
     9481 cggtggggcc gcgtcatcag gcatcttcac accgctggtg gtaccgttcc aggtgaagac
     9541 cacatcggta tcccagtcaa cctccgcgag gttgggaatc tcgccgtaag gagccttcat
     9601 gaccttggcg ttcagcttga gctgcttgtt gatgtccgtc acccagccct cgccgaagct
     9661 ctcccaggcc atggctgtga ccggcctggc ccccagcagg ctccacatgg cgcattcaaa
     9721 cgcgcccgtg tcgctccctg ggataatccc gatcagataa tcggccggga tggccgccac
     9781 ctcccgggtc agctcaagag cctgttgaat acgggctttg ccgggcttcg agcgatgaga
     9841 gcggcccgtc agggcccccg taagagcatg gacgctccaa cccggacgct tgcaggtggg
     9901 accggagctg aaattcggtg tactcggttt tttgtcaggt ttgtccacaa ctgttctcca
     9961 ggtgcaaatg acctccgctt ccgcaaaaca gcgggcaaaa aggttaccca aaaagcttga
    10021 tcacttcaac atcagggtag catcgaccct cgttggcggg tccagagatg aatgatgacg
    10081 gtagaattga gaatggggca tgctttgtcg tcgctcagcc ccccgccggt aagcttcgga
    10141 agcatcggcg aacccgcatg aaacacctga ttcacacgtg tctttcaacg cctttgcggc
    10201 gttttttatg caagtgggga ttctacttgg ccgccgccca gcggacggca ttgcggagca
    10261 cccgccggac gtcggggttg tggtaaatcg gcagggtctc gtggccgggg cggaagtaga
    10321 agacccggcc tttgcctcgc ttccagcagc aaccgctgcg aaacacctcg ccgccctcga
    10381 accacgagat gaagaccaac tcgtcgggcg cggggatctg aaaccactcg ccgtacatct
    10441 cagtctgggg tagttcgatc ttctcgtcga taccgccggc gatcgggtgg gtcggatcga
    10501 ccacccacaa gatctctttc ttgttgtcca cccgccactt gagcccgcac ggagtgccca
    10561 tcagccgctg aaaaggcttg gagtagtgtg ccgagtgcag cgcgacaaac cccattccgt
    10621 tccagacccg ccccagcact ctctcgacga cggcatccgg cacttcgtga tgtcgcatgt
    10681 gcccccacca gacgagtacg tccgtgaccg ccagaacgtc gtccgtcaac ccgcattccc
    10741 gatcccgcag tgtggccgtc cgcacttcca ggccgctctc ttcgcgcagc ccggccgcaa
    10801 tcgcaccatg gatgccgtcc gggtagatct tcggaacaac ctcggtgttg tattcgtgcc
    10861 agcactcgtt ccagacggtg acgcggatgg cgctcacggt gtactcctct ctagcggctt
    10921 cttgtctggc gacaatatac cgcgaggcac acaagcctgc gagaagacaa ggctgaaggc
    10981 ggaaggatga gtcaagacag gatggagtgc ggaacagatt ccgcaaacaa aaccggcccg
    11041 gaaggattcc gggccgagcg gtctagtagt ctaatagtcg aacagtctaa aagtctagca
    11101 gcctacatct cattgaacgc ttcccgcaac ttcttgaggg cctcgcgttc gagttggcgg
    11161 acgcgctcgc gagtcaggcc gatcttcgcc ccgatatcct tgagcgtcat cggggcctgg
    11221 tgctcgccca tcccgtagcg cattcgcagg atggtcgcct cgcgttcgtc gatcttctcg
    11281 agcagccgct ggatgacttc ggcatcggcg ttgctgaacg ccgccgcctc gggcgagggg
    11341 gtccgagaat cggcgagaat atccgacaga ccgaaggagc cttcggcgcc tcccggctgg
    11401 accggtgccg agaaggcctt gaccgcgcgg cgaatgatac gcagcttgcg ctccgagagc
    11461 ttcatgtgct cggccatctc ttgcagagag ggatgccggc caagctggtt ctcgagctcg
    11521 gcccaggcct tcttccagtc ggcgatcatc gcgaccatgt aggccggaat gtgcaccggt
    11581 tgggagctgt tgatcaacgc tcgcttgatg gcctgcttga tccaccacga agcgtacgtg
    11641 ctgaagcggg tctccatggt cggatcgtag ccctcgaccg ccctgagcag cccgatgttc
    11701 ccctcctcga tcaggtccga caacgacaac ccgcgattga ggtaattctt ggcgatgttg
    11761 accaccaggc gcaggttgga caagaccatc cggtcccgag cccgggcggc ctcctcttcc
    11821 agtttttcct tctcctggag cgagattcga ccctcccgca gcaactcgcc ggccctgatc
    11881 gatttctgga tgatcgaacc cagttcgatc tcctccttgg ccgtcagcag gggagcctca
    11941 tcgatctgct tgagatagtg ttgcaatccg ctttcgaccg cgatggcgct accctccctt
    12001 catcgtccgg gtctctgcga ccctgcccgt ccctgcgcga ccggagacat ccgctgtctc
    12061 cgaggaagga tgaagtcaca ctcgggccgg accggcactg cccgtcccca tcggcaatcg
    12121 accggacccg gattaaccgg cccctgatct tcgccgaggt ccaaacccgc tgcggcgggg
    12181 ctcgcgaggc ttcggtcggg gctcctggcg atgcgagcac tcactcgccg gcaacctgtt
    12241 catcggttgt ctggcccccc tcggcactct gtgggctcga ctcggccggg cggatgggcg
    12301 agtactgaat cacatctgtt gattcggcgc ccggaccgtg caagccgccg cgacgaggct
    12361 tcggtttgct cgtatcgcgg gtgccgtcgt cggccgccgc ccattccgcg cgtttcttgc
    12421 tcagaccgat cttccgctct tgaggatcaa cccgcagaat cttcacctcg atctcgctgc
    12481 cgatcttgac ctcgtcatgc gggttctcga ccttatgatc cgccagctcg ctcacgtgca
    12541 acaggccttc cagctccggc tccagctcga cgaacacgcc gaagttggtg atcttggtga
    12601 ccttgccctt gacgatctgc cccggaagat aagtcgaggg aatcgcgtgc aaccacgggt
    12661 cttcggtgag ttgcttcgtc cccaaggcca cgcgcatctt ctcctggtcc accgaaagaa
    12721 cgacgcactt gacggtgtcg cccttcttga gcacctcgga cgggtgactg atcttcttcg
    12781 tccagctcat gtcggaaacg tgcagcagac cgtcgatgcc ttcttcaatc tcgataaacg
    12841 ccccatagtt cgtgaggttg cgaaccttgc cttcgatgat cgtgttgggc ggatacttct
    12901 cggccacgcg ctcccacggg ttcgtctcga tctgcttcat gccgagactg atttcctgct
    12961 tttctttgtt gatgtccagg accacgacct cgatctggtc gcccaggctg atcagctccg
    13021 aaggatgatt gatccggcgg gtccagctca tctccgagat gtgaaccagt ccttccacgc
    13081 cgggttccag cttcacgaac gcgccgtaat tgaggatatt gaccacttca cccttgattc
    13141 gcgagttgac cggatagcgc tgctcgatgc tgtcccaggg attctcggtc aactgcttga
    13201 gccccagggc gatcttctcc ttgtctttgt cgacgttcag aaccttgacc tcgatctcct
    13261 ggtcgatctt gagcatatcc gagggatggt tgatccgctc ccagctcata tcggtgatgt
    13321 gcagcaggcc gtcgatcccg cccaggtcga cgaacgcccc gaactcggcc aggttcttga
    13381 cgatgccctt gcggacctcg ccgacctgga tctcgccgag gaccttttcc ttggccgcct
    13441 gccgcttctc ttcaatgtac ttgcggcggc tgataacaat gttgcgacgc tcggtgtcga
    13501 tcttgaggat cttggcgtcg atatccttgc cgatgaactc accgatatcg ctcggccggc
    13561 gaatatccac ctgcgaggcc ggcaggaaca ccggaacgcc gatgtccacc aataggccgc
    13621 ccttgatttt cctcatcacg cggccgcgaa cgtcgtcgcc ttccttggcg gtctcgacca
    13681 gacgctgcca gttgagcagg cggtcggcct tgcgcttgct gatgaccacc aggccgctgt
    13741 cctactcgac cgtttcgagc cagacgtcca cttcgtctcc aacgtcaatc gaggacggat
    13801 cgtcccactc ggaggccggc accacgccct cgctcttgag accgacgtcg acgacaacgt
    13861 cccccccaac caagccgacc acgcgaccct tcagaatcgc accggtctgg aagttgccca
    13921 gggtggtgtc aatggtcgtg gccagcgcct catccgtggt ctggccgccc atcatctcag
    13981 caagttcctt ttcgaacgaa tcctcgttca catccagaga gttgatcagg ttgtaatcta
    14041 ccatctatgt tccggaagcc ttctgtgcat caggagatcc cgcgaggcaa aaactcacag
    14101 ccgtgacagg cctggtggac ctgcctcgga cgtccccatg aacgtcggcg acgcggcgca
    14161 aacctcttgc gctcttcccg gtcgtgccga aacggatact ataggcgaaa gccgcccttt
    14221 aagccaaccc cgcctgcgcg gcctgaaaat gctcacgcag ccgggaggct gatcaaaacc
    14281 ttgcggccgg agatccgcgc atcttgcgag cgcgctgctc cccgattgca gtctttgcct
    14341 gcacgccgcg aacaggcggt ctgcgttagc tcaggtgcgg aaagggttta cggcatggct
    14401 ggacgtccag gcagattgaa gtgggtgcgg cgtatccttt gcgctgccgg cctgctcatc
    14461 ttggttaccg gcgttactct ctggctgatg ttccagcaca tcccttcgtg gtaccgcccc
    14521 ccggccatcg ccccggccaa ccatcagcga atccgcaacg actgggccgg cagccgcgac
    14581 agccttcaac aggctatgtt gcgcgaagaa cctttcgagt ttgccgtgac tcaagaccag
    14641 atcaattcct ggctggccat tcgcgaggcc atatggcaat tgtccagaga atggctgccc
    14701 tccgccttgt ccgaaccctt tgtacggatc gagaaagaca gcttgcgcct ggctgccacg
    14761 taccgcagcg ggagtattcg aaccgtgctc tccgcccgcc tcgaaatcgc cctccgagcc
    14821 gacggaatat gggtcaggct ggccggcctg gacgccggct ctctgggcgt tcccgacgcg
    14881 tgggtgcgcg agcggttgag gttgatcgac ggccgggcat ggtcgcccgg caagaaaagc
    14941 cgctatcaac tgggcgggcc gcctctaccc tccctgccca cgcttcccga cggagccgtc
    15001 ttccccgcga cctgggtctg gaaagaatca gaattagcgc tgccctttga gatcgccggc
    15061 ctgcgtttcg agccgggggt gctcgtggtc agcctgcgac ctttgccccg ctaatccagc
    15121 cttgtgaacc gccaagactc cccgctcggt ccgtcagcct gcagccggaa ccgcccggaa
    15181 agccgctccc cgctcaggat gacgacatat gatcgagagt tgtcctccag gatacgatag
    15241 gtccctcgat cgcttcgggt cacgtacccc cgatttctgc tgatcggacc ttcatagtcc
    15301 aagtagtcat acctgtggtc cggcaggcga cgggccgaaa tctcttgcgg acccggtccc
    15361 gcggccgacg ggtcggccgc cagttgccag gtcgccaaca cggaccccgt atccagcatc
    15421 agatcccaat ggaccccgtc ccgagtgtca tgtctcaaca gaacgaaggc agctccgggt
    15481 tcacccatgc gtctattata tgccgatgta tccttgatgc ccgtccaagc ggaggataat
    15541 atcccccgac agacacggat gataccctgc ccgggtcatg gaggtgatca ctcatgatgc
    15601 gatgcacatc gcccgtatcc atggtgacgg tggcggtcgg gctgtcgctg ctcgccgtca
    15661 tgactggttg cacgccagat gtgacggata tccgcaacgt tgggatcaaa caataccagg
    15721 ccggacttca gattgaatcc atggcgacct tccgccgagc gctcgaactg gcccctaacg
    15781 atgccgtcag caattactac atgggccttc actacaagaa tcgcgccgat cgcaagttct
    15841 ccgagggtga cctgaccgga gcgtacaagc agctcgacac ggccattatg tacttcaatc
    15901 aggccaccac gagcatcccc agcttcatcg aagcaatcaa agaaaagaaa gacgccttgg
    15961 cacgccgcgg gaagtatgaa caagcggtca acatggccga gcacgtagct cagcggatcc
    16021 cgggtgacaa agtgacgcag tacgttatcc tcggcaatct gtatcgcgac gccggtgacg
    16081 ccgataatgc actgcgagcc tacaagcagg cccttgccat ggacccctcc tccaccgagg
    16141 cccgggtcga gatcgagcgt ctttacgaga gagcccgccg gccccggtaa tccgtggccg
    16201 cctgtcctca cgaatgctca acggggaacg gaagcacgcc gtgccttacc tgcggccctt
    16261 tcgggcaagc agatctctta cctcggcgaa gctttcgggg ggcaaaaggg cctcgcgaaa
    16321 gatggtttgc atgatttcat cggtcagccg ctcgaaatca cgcgggcgag gtgagacgac
    16381 gatgcctgcc aggtcggctg cgccgggact gataagaaaa tcatcctcgc ccgttccgta
    16441 aaaccgcggc cgatgcgcgc ctcgtggaaa aagccaggta acccaacgct cctcggtata
    16501 ggtcgcgaaa aggttaagca tcggctcggg ctcgaccggc cgcacctctc ccaagacgat
    16561 caccacctcg cgcaacgagg cggccatggc atctttcgag tcgcccatca gcacgatcac
    16621 cggcaaactg ccgggacgcg tgacggcgat ccgcacaggg tcgtcacgaa cccattccag
    16681 ccggcgttgc ccgtcggcag gatcaccggc cgccaactgg gtagccagtt gagtctcaaa
    16741 cggcagggcc cccgcccgcg ccgcctgaag atgaagatgg tcaggagccg aagcaccact
    16801 ggccggaccg ttatagaaaa cggtgtaccg gccgtcgaga tccctcgaca tactgagcag
    16861 cccctcgatc gcgggaccaa cccgctgcgg ttcgtgatcg atccatgcca ccgtgtagtg
    16921 cggctcaaac agcggcgcag gattgcagag aatgagccag cggtcgcggt aaggcactga
    16981 agcctgagcg accggacgat tctcggtgca cagaaaacag ggtcgttggg ccagcgactc
    17041 cgcgtccacc tttgccccgc tgcttttgat ccgcgccggg ttgcattgcg ccgttacccg
    17101 ccagccgttg acgtgaaagg ttcgcgtccg cgcctgcctc agcgccgcca gtccctcggc
    17161 cagcatctcc cacgtttccg tttgccgttg ccaaagagct gaaagctgat acgccagttc
    17221 accgggaccg gtcgcttgct gcttgtccat cgagttctga tctccacatc atatcccccg
    17281 taaccggccg tccccccgtg cgcccccatg ttagcacgtc ccgccacgca acggtaatgc
    17341 ctcgacccga ggcgttgctc ccggcggcaa ccacgcagtt catctctgtc ctgcgatgat
    17401 caccagattg tcgcgatgga ccgcctcatc gaagggtttt gagcctaacg cggccgctat
    17461 ctcgcaagac ttcaggcctt tgatcatcgc aagttcggtc gagctgtagt tcgaaatgcc
    17521 gtgggcgagg acgcggccat cgggaccggc gatctgcacg atgctgccgc ggtcaaattt
    17581 gccttcaatc cgggtaacac cacgggccag caggctcttg ccctgcgtgt gtaccgcgtt
    17641 ggcggcgccc gcgtcgacga cgatcacgcc cgtcggccga gccgccccca cgatccatcg
    17701 ctgccgagcc gacaacttgc gtgcggccgg ggcaaagatc gtgcccaccc gctcgccgtt
    17761 gagcagcctg agcaacgcgc cgggctcgcg accgttggcg atcacgacgc actcgccggc
    17821 ctcgctgacc attcgagccg cccccagctt gctgaacata ccaccgctgc caagcgggga
    17881 tcgttcggcc cgggccagcg acttcacttc ggtgtttacg gccggcacaa gatcgaccag
    17941 ttgccctccg gaatccagta acccgtcaac caccgtcagc aagaccagca ggtcggcacg
    18001 cagcaggttc gtgaccatcg ccgcgatcgt gtcgttgtcg gcgaagcggt cgagctcgtc
    18061 gatcgccacg gtgtcgttct cgttgatgat cggtacggcc ttgagccgct gcagggcgga
    18121 gatggtgttc gagatgttca catagcggtt ccgctgctcg aaatccttcc gcgtcaccag
    18181 cacctgaccg accagcaact catgccgggc aaactcccgc tcgtacatgc tcatcagcga
    18241 aggctggccg atggcggcca ccgcctggag caccggcatt gaacgaggtc gcctggccac
    18301 gccggtcttg cccatgccgg ccccgaccgc cccactactg accaacgtga ccttcacgcc
    18361 acgctcgatc agggtcgcaa tctgatcggt gagcgacgcg ataaccgtct ggtcgagctt
    18421 gccgttctcg caggtcaaca ggttggtgcc cgctttgacc acgatgctgc gaacgccggc
    18481 catgatctgt ttacgaagcg tcgtgctggg cacgttccat gatctccatc aggtggttca
    18541 ccgttcgctc ggtggcacca cggttacgtt tgacgatttc ctggcctcgg cgggccatct
    18601 cccccgcggc aaccgggtcg tcgagcaggt cgctgaccgc ctcggccaac cgctccgccg
    18661 cgtcaggagc cgacgaatcc accgaaagaa tgcgaattgc ccggccggcg tcaagacgct
    18721 cgacggcctc ggcaaaattc tccgtgtgcg gacccacaat gatcggccgg cccaatccgg
    18781 caacctccat catgtccgag ccacccatgt ccgccaacgt ccggcccaca aaaacgacgt
    18841 ccgccaaggc atagaacttg cgaagctcgc ccatggtgtc gccaagccga accgccggtg
    18901 attcctcacc tgacgagccg ccgggcacat gtgctagggc agcgtcgagg tcgctgcggc
    18961 gaccgagacc tgccgcatgg tcgagcgcag gacggcaggt ctccgccgcc tccggcggct
    19021 ccgaccgtgc cctacccttc tcgcacactt ctccggctgg acggtccccg tccggacacc
    19081 gactccgccg cagacacgcg aaacccgact gccggatcag ctcggcgacc tcgtcgaatc
    19141 gctccggctt gcgaggcacg atcatgagct gaagctgggg ccgtcgatgc cgcaacaaag
    19201 cataggccct gaggatgatc gcctcttcgc cgtcgccggt gctgccgcat acccacaccg
    19261 gccggttgcg gtcgatgccc atggctcggg ccagttcatc cctgccgggc aacgaatcgg
    19321 ccacttgggc ggtgtcccat ttcatcgaac cggtcaccgt gacccgatcc gccggcacgc
    19381 ccagccgccg gaagcgatcc gcgtgctcct cggccaaggc ccccacccac gtcagcgagc
    19441 cgaacattcg gcgggcgatc ggcatgatcc aacgaaaccg ccgcatgctc ttctgctcgg
    19501 tcaaccggcc gttgatcacc accacaggaa tgttcctcac cttggcctgg ctgatgaatt
    19561 ggtaccacac ttccgcttcg accaggacga tcatcgtcgg agcgatgcgg tccagagccc
    19621 gccgcacgaa ccgactgaaa tccagcgggt accgcacgac gatcaggtct ttgaacagct
    19681 tgcgcgcctg gtcgatgccg gtgtcggtgg tcgccgagat gacgaaatcg agctcagggc
    19741 aacgggcccg ccacatctcc accagcccgc ggatggagtt catct
//
LOCUS       DAOTPW010000096        19671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2829_length_19671_cov_5.792567, whole genome shotgun sequence.
ACCESSION   DAOTPW010000096 DAOTPW010000000
VERSION     DAOTPW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19671
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2829_length_19671_cov_5.792567"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="P5316_10380"
     CDS             <1..1215
                     /locus_tag="P5316_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRR85405.1"
                     /translation="DGPAGTQVDSTGRVHGWTPAPAQAAQAFTLTVRATNSAGSDDES
                     WLVQVISPPADTIAGFPFNSGAEGWTLQAWRSSIQYDPGYAIWDSAGGNPGGNLKAGG
                     NGATNNADSCTREGGLATRQISTVGFTNIQIEYDVMAALNSPPAAGCDGGCVADILEG
                     SCEDKLVVYYSTSGTGGPWAAAEQLNEGTDLPTTWTHKVIRLAAVPAANDNPDFAVQF
                     KWQFNTITDAGRIDNVIVKGSPLDNNEAPQVDAGPDQTITLPNSANLTGTVSDDGLPN
                     PPGEIIVTWSKQSGPGTVTFNNVNALQTTASFGTAGVYVLRLLATDSQLTGEDLVTIT
                     VNEPTPADFDGDGDVDQTDFGIFQACLSGTAQTPSTGCEKADLDGDGDVDQSDFAQFY
                     DCLGGADSPPGC"
     gene            1386..4565
                     /locus_tag="P5316_10385"
     CDS             1386..4565
                     /locus_tag="P5316_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HRR85406.1"
                     /translation="MPTRYLTLILWIAHGFAGISFAVDVGKYDTFETNVMNYNSYSNP
                     FQNVTLNATFTSPSSQNTSIRGFYDGNNTWRVRFMPDQLGTWTYTCTFSDGAPGKSGS
                     FSCVASNLHGPLKTDTATPVWFEHADGTPFFMRAFHLWRPETFTEPVLEQTLDFMKAN
                     GLNVVDGPHLANWGTGLNQIPWEGSDYTNYKLSVWRQLDVYVRKCAERGIVVFPFSIL
                     GGTNGIPKMSTWDQRQRFIRYWVARWGGYWNVAYCPVSEYAEAYSTAEGMQILNEIYN
                     SDGGRHPVSIHPGNTLAEPATEIKTMTAYTYHTVQDKLDDWNPGRYQQLITLYNTAQK
                     PIYAQETLWEGNKYQPDSSRDMNNMRKAAWITTTYGGQISYADECMPGHLPQDNNETL
                     CYAMHGASVSPQGWFYPYFSHLGSFIESVPFWRMSLQPGLASTGNCLAKTDYWYVAYT
                     MGGSFTLNLSAAGSALIGRWYDPRNGTFGSPFSVSPGGVRTMTPPDSNDWVLYVCRDL
                     DDTPPAAVSSFTATPGEHRNTLIWTNPSSTNFIATVIRCKTTGYPTSPTDGEPVVNLA
                     NMPGSSHTYVHSGLSAGVTYYYTAFARDDEYHYSSGVQAGAIPHDHKRVSVDLGTTDI
                     EQGIYNVQPSDGDTVPADVGGRNCRRNAVAGNDNYMYFNVTDAYMYAGYRPVCYVSVE
                     YYDTGTGILTLHYDSTSGAYTSGGTITLTDSNTWKIREFALTDAYFGNRQNGGADFRI
                     ACASGASFYLNEVSVANVSTVDSAPVINPVGWNPDAAYVGAEYVRQLSLAQGRPLPSW
                     TILQGPGGAVIDDSGLLSGWTPSAGEDGQTITFLVEATNSQGSDTEEWSVTVGPAPLA
                     TYEAEGASMGHLAGTAIPGGWRITTANPNTFSTYGPYTSALPAGPLTARFWLRVDNNT
                     ADNNGICRLDVYDADGATLLAGPVTIARRQWTTSGVYQPFELPFANPGDAHRLEFRTY
                     YMGYAQLDQDRVDIFSGGDLPATADFDGDGDVDQSDFGYFQACFSGSASPYESGCEKA
                     DLDRDGAIDQNDFAVFQSCLSGPGNPPDC"
     gene            4780..5535
                     /locus_tag="P5316_10390"
     CDS             4780..5535
                     /locus_tag="P5316_10390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRR85407.1"
                     /translation="MINPASDSRPRPFKRVVVLGESTVEGGGWVPAQEDRYADVLVSL
                     INRVQAEPVEYVNCGIGASVISPKSPGYAASRKPSAMERYQTEVIAAGPDLVIIAYGL
                     NDMRAGMNVNDFISELETIVRDVQSSCRALIVLVNVYHMTGYGWYPPFDRGSVEATLV
                     YNDAIRQLAERTGSLYADVYSAEARADWVVHQDGVHANKVGNLLIANKIFEILATHCS
                     GLSEAVRRHDENTEWTKAVRQMRHAKVEPVRKP"
     gene            complement(5564..7138)
                     /locus_tag="P5316_10395"
     CDS             complement(5564..7138)
                     /locus_tag="P5316_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR85408.1"
                     /translation="MTSIIAKMTRDHDGKRLSRSIGSAGRLGGVLLLGLLSASLWAAE
                     QSRSGPNLLFIWTDEQRADTMAAYGNTKIHAPNLDKLASESIVFRNAYVTQPVCTPSR
                     SSVMTGLWPHNSGCTTNNVPLPRQVLCLPELVGDRGYRTAYMGKWHLGDEIFPQHGFE
                     QMVSIEDGYARYYGPGRDRTQRSDYHRFLLSKGYKPNQKDGTFGREFASRLPIEHCKP
                     KFLETRACEFLRRHRDEPFILYVNFLEPHMPFFGPLDREHRLDEVFLPANFSDPLEDN
                     EPLRYRLMGEYCARVYGRDEAAFKELTRKYWGLVTQVDRSIGEILKALDELKLSDKTI
                     VVFTSDHGDMMGSHKLVTKTLMYEESVRVPWLMRVPELGRQQRMITSPVSHIDLVPTL
                     LDLMGMRVPDGLAGYSLVPLMRGGKAAEDHVFIEWNPGTGVHVVEGGSKIASQAEIDR
                     IREARIRTIVSPDGWKLCLSDTDRSQLFNLRDDPGETRNLFDSGRHEDVIVRLTERIR
                     RWQQKTGDTMKLPDGR"
     gene            complement(7216..10386)
                     /locus_tag="P5316_10400"
     CDS             complement(7216..10386)
                     /locus_tag="P5316_10400"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRR85409.1"
                     /translation="MRGTGAVLMLAIVVTLATMTASATGALSPLRLRCEYAVNPLGID
                     AARPRLSWGLHADRRDEKQTAYQILAAGSSERLDRDQGDFWDSGKVSSDQNVHVVYDG
                     SSLLSGARVYWKVRVWDRDDQVSAYSEPAWWEMGLLKPSDWTARWIACDELKGLGAGL
                     QLGQWIWVPDAPAQVPTAFFRRSFDLAPSMKIAEAIIKLNADNAFELYVNGGFVGGCR
                     YWDMPREYDVTPDVRPGLNVIAVKVVNSVDPGGLRAALRIRFVDGRLFDLASDADWLV
                     NTTEQTKWQEPDFDQSTWQHAVVLPADKADRWKQIQDPFGPPRSVCLRKVFALSRRVV
                     RARACASGLGLYELYVNGHRVGDDVFAPGWTDYSKRVQYQVYDVTALLHEGENAVGAI
                     LGNGWFSGDLGWTRKPPFSQGPLRFILQLEIEYDDGTRERVVTDRTWQTHTSPITRNT
                     FFNGETYDARLEMPGWATPTFIARDWVPAIELEQGGKLVAEQCEPIRVTEELRAVQIT
                     EPAAGVYVFDFGQNAAGYVRLSVRGPRGTWIRLRFAEILRPDGQLYRDNLRAAEATDY
                     YILKGEGQEIWEPTFTYHGFRYCEVTGYPGKPTVDSLAFRVLHSAAAPAGRFECSNPL
                     LNQIWRNVTWSQRSNMYSVWTDCPQRDERMGWTGDAVAMAPTACWNMHMARFFTKWMR
                     DLTDGQGEDGGVPDIAPAVYGSGAGSPGWSDAITTVPWTVYQFYGDKGILEENYEAMT
                     RWVEFMGRHSKDHLYQRDGYGDWIAPAQSPKPPMAAAYYYHSTDLLAKAAAAIGRTDD
                     ARKYADLAGRIAEAFNHAYFDKQTRQYQGSTQTANVLPLYFGLVPPAETAAVVQNVVR
                     DIESRGDHLSTGFQGTAYLMPVLARYGQQEVAYRLAVQTTYPSWGYMVKQGATTIWEL
                     WDADKRGPEMNSRNHAALGAVGRWFFEGLAGINIDPAEPGFRHIIVRPTPVGGLTSVE
                     AEYESIRGLVASRWRAEGERFTLNVVVPVGSRATVFLPAAKASRVTETGRPVESAGGV
                     HLVREEGGAVVCEIGSGSYEFEVVSP"
     gene            complement(10529..11989)
                     /locus_tag="P5316_10405"
     CDS             complement(10529..11989)
                     /locus_tag="P5316_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR85410.1"
                     /translation="MARAMVCKVMWTLVLVALPASLTGAKAADQQVASPGAQSSLGFV
                     KEPAKKVPVAYDVDVVVAGGGISGVFAALAAAREGATTVLIDRFGYVGGSFGPGMMSQ
                     QGPIAEHGDAPASGPAGDPVKGGLAGLPKEFSREYFALGSGTPERSLADSNKAAYVAL
                     KMLKEAGVTLMLSAYVADPIMEGNKVCGVFVENKSGRQAVKAKVVIDATGEADVARRA
                     GTPILQPKAETYDLDHHSPTGMGIWAVVAGIDPARFKGMEASEFEAKKDIGDLAQVGC
                     SGLVEIGPERHMRGIKAQLVRPHPKVDAGNAEHISLLEAGVRMYIFEFVQHCRKNVPG
                     CEDAYLLFTAPYLGCRGGPCIEGEYTLTMQDCREGKRFDDVIYRYGEPRAFRWMNEHG
                     RVEWTDVPYRVMIPKKTDGLIAVGRSASGIPDTLLRNREGVMYMGQAGGTAAAMAAKS
                     GIEPRAVDVKQLQRKLLKSGFHLGDRARLAELGLVD"
     gene            complement(12054..13628)
                     /locus_tag="P5316_10410"
     CDS             complement(12054..13628)
                     /locus_tag="P5316_10410"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HRR85411.1"
                     /translation="MIPSRQRVFISVFASVVLLINVRLTADQPRQLFWGDIHNHNAVG
                     YGKGSLQRSYEIAESHLDFFAFTAHGHWHDMPIMPENKHEKWVNGFAATREHWDEVRR
                     MAKERYKPGRFVPFVAYEWHSSRFGDQCLIYPSDVDAPLEYFDHISKVQAFAKEKGAL
                     LIPHHPAYRWQWRGTAFEFLDTDVSPVVEIFSEHGLAEHDRGPFDYVRHSNGGRWTPR
                     TLRALLARGAHVGVVASTDNHSGYPGAYREGLVGLYANELTREGVFEALRARRSFAVT
                     GDRIALDFRVNGHWMGDILPETPNRQVTITASGWDEIASIELVKNGRVVARAFPTDAD
                     VHSPPWSSPVLCRVEFGWGPWGDLAMTRICDWDFELEIIGGRIVEAQGCFRAGPFDED
                     RRNRVEKLSEKSYRVRSYTSRKEPLGDLVNNSVVLKIEGGPQAVLKCTMTRPVAMKIE
                     KSLKDLSSENTLEFTGPFTSESMVFQRLVFAPHYTGRLEFSDGPDEGAKEDWYYARIV
                     QANGDLAISSPIWVRN"
     gene            14067..16208
                     /locus_tag="P5316_10415"
     CDS             14067..16208
                     /locus_tag="P5316_10415"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR85412.1"
                     /translation="MRANRRIGTILLLLVSACDRGALAVDLYVAPSGKIEWSGTLPAA
                     NAEHSDGPLPSIAAARDLIRSRRASGMWTDEAITVHLRGGVYYLEETVVFEPRDSGTP
                     GKPIVYAAYKNEQPILSGGRVIGGWRDAEINGRPCWVVEIPEVKEGKWYFRQLFVNGR
                     RCPRPQVPEQGWYQFAGIPPHSERYLKRENVRDKANQNDRACYRPGHVKKWHNLGDVE
                     FVCLMSWDEAHLFAANLDEATRTIEFTQPSYLGADKVITDDKNGRYYMLGVREALNKP
                     GQWYLDRVSGMLYYLPVAGETPDNTTVVAPRLNELLCVLGDEPRNPLEERNKTFWTGP
                     RLIGQGPTPRPIEHLALRGLTLKHNNWFLPPDKSAGGQAMARLPAALFLLNARNCRIT
                     DCTVSQISNWAIELGAECRDNVISGCTLTDLGAGGVKLNEDSHHNTVTDCEISNGGLV
                     FPGAAGVWVGHSWANTVSHNHIHHLYYTGISVGWVWGYSRPSKAACNIIEYNHIHHIG
                     RKLLSDMGGIYTLGSSPGTVLRYNLIHDVEAFAYGGHGLYNDEGSSFVLLENNIVYRT
                     SHESYHQNYGQYNVIRNNVFAYSADGTVNRSGQNDPFSFTIEHNIVYSTLPRMLDNGW
                     RWNKSGNFKMDYNLYWREDGQPFDFPGDRSFEQWQQWWDQDRHSIIADPLFVDPKNGD
                     FRLKAGSPAERIGFKPIDTSRIGRLKSHTPG"
     gene            16359..16607
                     /locus_tag="P5316_10420"
     CDS             16359..16607
                     /locus_tag="P5316_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85413.1"
                     /translation="MRSNCKVRDPNGTSAGDLLESHRVLSDFFHWIVAHPRVLRVVDL
                     LNIWGLIGIGLAPFLNPLTRLACAFGILLLVLYYVSFR"
     gene            16614..17174
                     /locus_tag="P5316_10425"
     CDS             16614..17174
                     /locus_tag="P5316_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR85414.1"
                     /translation="MKRREAAGIVGRRREAAGRTADENRNDAIAREQNLSPEQRLAFH
                     QAESGPIMEQLRAWCIRQFDERLVEPNSALGQAISYLLKHWEKLTLFLRQPGAPLDNN
                     IVERALKRAILHRKNALFYRTLNGARVGDLFMSLIHTCQLNDVDPFDYLTELQRHAED
                     LAAQPENWMPWNYRKTIQETDADIPG"
     gene            17223..18794
                     /locus_tag="P5316_10430"
     CDS             17223..18794
                     /locus_tag="P5316_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRR85415.1"
                     /translation="MLTGRTQRSYVHGMQYIDERAVMRDHQADAKPGDNQMGKDYYYL
                     LQELYTVAGLADCRGWMEEMYVYDTYGGATLYDWWPGDVNRNGPVTAGDMALVSARVG
                     AGWDEPWCDANLDGDVTAADVSEIAALIPVSSSPPPTINYSSVDNPYFFTGRTTDTLH
                     ASALLVSEDPDFKRIQDNRNRMYDPKHGRWLQRDPAGYVDGVNLYSYARSNPTGGLDP
                     LGTWDYRVHTEKTRSWAIDEAMTETGAARVAHYDEATDTFAYFGEYAEQSWTPVPGGR
                     SQGYHFNAYRYGVDSRHQHRDEDYDKALKACLDKDDPETASRWLGHSLHALQDWYAHG
                     EYNSGDSDTFITHGPYYDDPEYDAAGPVYRVYHVTFESSYNWPGDDGYKIPPAYPLYW
                     FYKYDAFDRPNGSTPVQARSDGRPPKVYGRWLTRLQSGYGGRAGEPSYLSVFWEARWL
                     LWERGNKRVSGTEMESRSHIDKFLRAICTGCNNGKCPKCQCHFIQFGPLATSSAGSSR
                     RCGEECAAESEGTER"
     gene            19363..19662
                     /locus_tag="P5316_10435"
     CDS             19363..19662
                     /locus_tag="P5316_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRR85416.1"
                     /translation="MNERSSNKPVPLLALAAALVIPGFLPQSAHGMYDPKHGRWLQRD
                     PAGYVDGMNLYQYTGSSPVVLADPTGGCSKPPPPPCTCCCALADLSDLSYRFSYF"
BASE COUNT         3995 a         6293 c         5716 g         3667 t
ORIGIN      
        1 gatggtccgg caggcacgca ggtcgatagt accggtcgtg tccacggctg gacgcccgcg
       61 cctgcccagg ctgcgcaagc attcacgctc accgtccgag cgacgaattc cgccggcagc
      121 gatgatgagt cctggctggt ccaggtgata tcgccgcctg cggacacgat cgccggcttt
      181 ccattcaaca gcggggctga gggctggacg ttgcaagctt ggcggtccag catccagtac
      241 gacccgggct atgcgatctg ggattccgcc ggcggcaacc caggcggcaa tctgaaagcc
      301 ggcggtaacg gcgcgaccaa caacgccgat agctgcaccc gcgaaggcgg cttggccacc
      361 aggcagatct cgacggtcgg attcaccaat attcagatcg aatatgacgt gatggctgcc
      421 ctcaacagtc ccccagccgc cggttgtgac ggcggatgcg tcgcagatat tctcgagggc
      481 agttgcgaag acaagctggt cgtctactac agcacctcgg gaaccggcgg cccctgggct
      541 gcggccgagc aactcaatga aggtaccgat ctgccgacga cctggacgca caaggtgatc
      601 aggctggccg ctgtgccggc cgccaatgac aaccccgact ttgcagtcca gttcaaatgg
      661 cagttcaaca ccatcacgga tgccggacga atcgacaacg tgatcgtcaa aggctcacct
      721 ctcgacaaca atgaggcccc ccaggtggat gccggaccgg accagaccat caccctgccc
      781 aacagcgcga acctgaccgg cacggtctcc gatgacggcc tgccgaatcc gccgggtgag
      841 atcatcgtaa cctggagcaa gcagagcggt ccgggaacgg tcacgttcaa caatgtaaac
      901 gcgctccaga cgaccgcgtc attcggtacc gcgggtgtct atgtactcag gctccttgcc
      961 accgactcac agttgaccgg cgaggacctc gtgaccatca ccgtgaatga accgaccccg
     1021 gccgatttcg acggggacgg cgacgtggac cagacggatt tcgggatctt tcaggcgtgc
     1081 ctctcgggta cggcacagac accgagcacg ggctgcgaga aggcggatct cgatggagac
     1141 ggcgacgtgg atcagtccga tttcgcccag ttttatgact gccttggggg agccgacagt
     1201 ccgccgggat gctgatggag aatgaaggtc atgatacggt aatccgggaa cagaaacaca
     1261 cacaggagct accgctctcc cggtattgac cgaagcctgc aatcatggct ggtgtggagg
     1321 ctcatcggcg agcatcctgc gatggtctcc cgtctaccaa gtgctctgca cggaaggatt
     1381 cagcgatgcc gactcgttac ctgaccttga tcctgtggat cgctcacggg tttgccggta
     1441 tcagctttgc agttgatgtc ggcaagtatg acacattcga aacgaatgtg atgaactaca
     1501 actcctacag caaccccttc caaaacgtca ccctgaacgc gaccttcacc tcgccgtcgt
     1561 cgcagaacac ctccattcgc ggattctacg acgggaacaa cacctggcgg gtgcggttca
     1621 tgcccgatca actcggcacc tggacctaca cctgcacgtt cagtgatggc gcaccgggca
     1681 aatccggctc gttctcgtgc gtggcgagta acctgcacgg gccgctcaaa acggacacgg
     1741 ccacgccggt atggtttgag cacgcggacg gcacgccttt cttcatgcgg gcgtttcatc
     1801 tttggcgacc ggagaccttc accgagcctg ttctcgaaca gacgctggat ttcatgaagg
     1861 ccaacggact caacgtggtc gacgggccgc atctggccaa ctggggcacg gggcttaatc
     1921 agatcccatg ggaaggaagc gactacacca actacaagct gagcgtctgg cgacagctcg
     1981 acgtctatgt ccgcaaatgc gccgagcgcg gtatcgtcgt ctttccgttc tcgatcctgg
     2041 gcggtaccaa cggtatcccc aagatgtcca cctgggacca gcgccagcgg ttcatccgtt
     2101 actgggtggc ccgctggggc ggatactgga acgtggccta ctgccccgtg agcgaatatg
     2161 ccgaggccta ctccaccgcc gagggaatgc agattctcaa cgaaatctat aactctgacg
     2221 gcggccgcca ccctgtttcg attcacccgg gcaatacgtt ggctgagccc gcaacggaaa
     2281 tcaagaccat gacggcgtac acctaccaca cggtccagga taaactcgac gattggaacc
     2341 ccggccgcta ccagcagctc atcaccttgt acaacaccgc tcaaaagccc atatacgctc
     2401 aggaaaccct gtgggagggc aacaagtatc agcccgactc cagccgggat atgaacaaca
     2461 tgcgcaaggc agcctggatc accaccacct atggcggaca gatcagttat gccgacgaat
     2521 gcatgccggg acacctgccg caagacaaca acgaaacact ctgctatgcc atgcacgggg
     2581 cctcggtcag tccgcagggc tggttctatc cctacttcag ccatcttggc agcttcattg
     2641 aatccgtgcc cttctggcgg atgtcactgc agcccggcct ggccagtacg ggcaactgcc
     2701 tggccaagac cgactattgg tacgtggcct acacgatggg cggcagcttc acactcaatc
     2761 tctcggcagc cggctcggcg ctgatcggac ggtggtacga tcctcgcaac ggcaccttcg
     2821 gttcgccgtt ttccgtttcc ccgggcggcg ttcgcacgat gaccccgccc gacagcaacg
     2881 actgggtgct ctacgtctgc cgcgacctcg atgatacgcc gccggcggcg gtttcgtcct
     2941 tcacggcaac acctggcgaa caccgcaaca cactgatctg gaccaacccg tccagtacca
     3001 acttcatcgc cacagtcatc cgctgtaaga caacgggtta ccccacctcc cccaccgacg
     3061 gcgagccggt ggtcaacctg gccaacatgc ccggttccag ccacacgtat gtacattcgg
     3121 gtctcagcgc aggagtcacc tattactaca ccgcttttgc tcgtgatgat gaataccatt
     3181 attcgtcggg cgtccaggcc ggcgccatcc cccatgacca caaacgggtg tcggtcgacc
     3241 tgggcaccac cgacattgag cagggcatct acaatgtgca gccgagtgac ggagacaccg
     3301 tccccgccga cgtcggcgga aggaattgcc gccggaacgc cgtagcgggt aatgacaatt
     3361 acatgtattt caacgtgacc gacgcctaca tgtacgccgg ttatcgtccc gtctgctacg
     3421 tcagcgtcga gtattacgac accggcaccg gtatcctgac cctacactat gattcgacga
     3481 gtggtgctta taccagcggg ggaaccatca ctctgaccga ctccaatacc tggaagatcc
     3541 gcgaattcgc cctgaccgac gcctatttcg gcaaccgtca gaacggtggt gccgattttc
     3601 gtatcgcctg cgcaagcgga gcgtccttct atctgaacga agtgagcgtc gccaacgtct
     3661 cgaccgtgga cagtgcacca gtgatcaacc cggttgggtg gaatccggac gcagcctatg
     3721 tgggcgcgga gtatgtccga caattgagcc tcgcccaagg ccggccgctg ccatcctgga
     3781 ccattctcca gggacccggc ggcgcggtca tcgacgacag cgggttgctc agtggctgga
     3841 caccgtctgc cggcgaggat gggcaaacca tcaccttcct cgtcgaggcc accaacagtc
     3901 agggcagcga caccgaagaa tggtcggtga cggtcggtcc ggccccgctg gctacctacg
     3961 aggccgaagg tgcgagcatg ggtcacttgg ccggcaccgc catccccgga ggttggcgga
     4021 tcacgaccgc aaacccaaac accttcagta cctatgggcc ctacacgagc gctctgccgg
     4081 ccggcccgct caccgcccgc ttctggcttc gcgtggataa caacacggcg gacaacaacg
     4141 gcatctgccg actggatgtc tacgacgccg atggagccac cttgctggcc ggtccggtca
     4201 ccatcgcccg gcggcagtgg acgacatccg gcgtttatca accctttgaa ctccccttcg
     4261 ccaatccggg tgatgcacat cgtttggaat tccgtaccta ctacatgggc tacgctcagt
     4321 tggaccagga tcgggtcgac atcttctctg gcggagacct tccggccacg gcagattttg
     4381 acggtgacgg cgacgtggac cagtccgatt tcggatactt ccaggcatgt ttctcgggct
     4441 ccgcctcgcc ctatgagtcc ggatgcgaga aagccgacct cgacagagac ggggcgatcg
     4501 accagaatga cttcgccgtc ttccagagct gcttgagtgg tccgggaaat ccgcctgact
     4561 gttgaccctg gacgcgcatt cgcagaccgg aaccaggcaa tctctggctg cctgccccct
     4621 caacaacatg cctgaacagg agtcaccatg gttgcttcta caagtgcttc tgatgacacg
     4681 atctgtttct cccaactcag tcgacgctac gtgcgatggc ggcgatggat tgcctgcttg
     4741 cttgcgatcg caccagtgtc gtgcgggagg cctgctcaca tgatcaaccc ggcaagcgac
     4801 tcgcggccac gaccgttcaa gcgagtggtc gttctcggag aaagcaccgt cgagggcgga
     4861 ggctgggtgc ccgcgcagga ggaccgctac gccgacgtcc tcgtgtcgct catcaatcgc
     4921 gtgcaggccg agcccgtcga gtacgttaac tgcgggatcg gggccagcgt gatatcgccg
     4981 aagagccccg ggtacgcggc ctcgcgaaaa cccagcgcca tggaacggta tcaaacggag
     5041 gtcattgcgg ccgggcccga tctggtgatc atcgcgtacg gtctcaacga catgagagcg
     5101 ggtatgaacg tcaatgactt catcagcgaa ctggaaacca tcgttcgcga cgtgcagtcg
     5161 tcttgccgcg ccctgatcgt gctggtcaac gtatatcaca tgaccggcta cgggtggtat
     5221 cccccgttcg accgcggcag cgtcgaggcc acgctggttt acaacgacgc aattcgccaa
     5281 ctcgcggagc gaacgggctc cctgtacgcc gatgtgtact cggccgaggc ccgcgccgac
     5341 tgggtcgttc atcaggacgg cgtgcacgca aataaggtcg ggaacctgct gatcgccaat
     5401 aagatcttcg agatcctcgc aacgcattgc tcaggactgt cggaggcggt tcgccgacat
     5461 gacgagaaca cggaatggac caaggccgtg cggcaaatgc gtcacgccaa ggtcgagccc
     5521 gtcaggaaac cgtgacggcg gagaagcccc gagtgtctcg agctcatcgc ccgtccggca
     5581 gcttcatggt gtccccggtt ttctgctgcc atcggcggat ccgttcggtc agacggacga
     5641 ttacgtcctc gtgccggccc gaatcaaaga ggttccgtgt ctcgccggga tcgtcccgta
     5701 gattgaagag ctggctcctg tcggtgtcgc tcaggcacag cttccagccg tcgggcgaaa
     5761 caatggtccg gatccgtgcc tcgcggatgc gatcaatctc ggcttgggag gcgatcttgc
     5821 tgcccccctc aacaacatga acgcccgtgc ccgggttcca ttcgatgaaa acatgatctt
     5881 cggccgcttt cccgcctcgc atcagcggaa cgaggctata acccgcaagg ccgtcgggaa
     5941 cacgcatgcc catcaggtcc agcagcgtcg gaaccaggtc gatgtgactc accggagagg
     6001 tgatcatccg ctgctgtcga cccagctcag gaacacgcat caaccaggga acccggaccg
     6061 actcctcgta catgagcgtc ttggtgacaa gcttgtggga acccatcatg tcgccgtggt
     6121 cactggtgaa aacgacgatc gtcttgtcgg agagtttcag ctcatcgagc gccttgagaa
     6181 tctcaccgat gctacgatcc acctgggtca caagtcccca gtacttgcgc gtcagctcct
     6241 taaaagcggc ttcgtcccgg ccgtacaccc gggcgcagta ctcgcccatg agccggtagc
     6301 gcagcggctc gttgtcttcc aggggatcgg agaagttagc gggaagaaac acctcgtcaa
     6361 ggcggtgctc gcgatccagc gggccaaaaa acggcatgtg cggctcgagg aaattgacgt
     6421 agagaatgaa aggctcatcg cgatgccgtc gcaggaactc gcatgcccgg gtctccagga
     6481 atttcggctt gcagtgctcg atgggcagac gggaggcaaa ttcccggccg aaagtgccgt
     6541 ccttctggtt tggcttatag cctttcgaaa gcaggaaacg atggtagtcg ctgcgctggg
     6601 tccgatctcg acccggcccg tagtaccggg cgtagccgtc ctcaatggat accatctgct
     6661 cgaagccatg ctgtgggaag atctcatctc ccaggtgcca tttgcccata taggccgtgc
     6721 gatatccccg atcgccgacc aactcaggca ggcaaagaac ctgtcgtgga agagggacgt
     6781 tattggtggt acatccactg ttgtgcggcc acaggccggt catcaccgac gagcgggaag
     6841 gcgtgcaaac cggctgggtc acataggcgt tcctgaacac gatgctttcc gacgccagtt
     6901 tgtccagatt cggggcgtgg atcttcgtat tcccgtatgc cgccatggtg tccgcccgtt
     6961 gctcgtccgt ccagatgaac agcaggttgg gcccgctccg gctctgttcg gccgcccata
     7021 acgacgcgct cagcagcccc aacaggagta cgccaccaag tcgcccagcc gacccgatcg
     7081 accgcgaaag acgcttccca tcatgatccc gtgtcatctt cgctatgatc gaagtcattg
     7141 acctgtcctt cccgttaaag aagccttgtg gctactctgg ctgtcagccg cgcccggtat
     7201 catcagcctc cagcctcacg gagaaacaac ctcgaactca tacgaaccgg agccgatttc
     7261 gcagacaact gctcccccct cctcgcgaac cagatgcacg cctcccgcag actcgaccgg
     7321 acggccagtt tcggttaccc ggcttgcctt ggccgccggc aggaagacgg ttgccctgct
     7381 gccgaccggg acaaccacat tcagagtaaa tcgctcgccc tcggcacgcc acctgctggc
     7441 caccaggcct cgaatggatt cgtattcggc ctcgaccgag gtcagtccgc cgaccggcgt
     7501 cggccggacg atgatgtgtc tgaagcccgg ttcggccgga tcgatattga tgccggccaa
     7561 gccctcaaag aaccatcgcc cgaccgctcc gagcgcagcg tgattgcgcg aattcatctc
     7621 gggacccctc ttgtcggcat cccagagttc ccagatcgtc gtggccccct gtttgaccat
     7681 gtatccccat gaaggatagg tcgtctgcac ggccaggcgg taggcgactt cctgctgccc
     7741 gtaccgcgcc agcaccggca tgagataggc cgtaccctga aagcccgtgg acagatgatc
     7801 gccgcgggat tcgatatccc gaacaacgtt ctggacgacg gccgccgttt ccgcaggcgg
     7861 cacaagcccg aagtacaagg gcaggacatt cgcggtctgg gtcgagcctt gatactggcg
     7921 tgtctgtttg tcgaagtaag cgtggttgaa cgcctcggcg atccggccgg caaggtcggc
     7981 gtacttgcga gcatcgtcag tcctgccgat cgccgcagcc gccttggcca gcagatcggt
     8041 cgaatggtag tagtacgccg cagccatagg cggtttgggt gattgcgccg gggcaatcca
     8101 gtccccgtaa ccatcacgtt gatacagatg gtccttgctg tgccgcccca tgaactcgac
     8161 ccagcgagtc atcgcctcgt agttttcctc gagaatcccc ttgtcgccgt agaactggta
     8221 caccgtccag ggcacagtgg tgatcgcgtc gctccagcct ggcgaaccgg ccccgctgcc
     8281 gtaaacggcg ggggcaatat cggggacgcc gccgtcttcg ccctgcccgt cggtcaggtc
     8341 acgcatccac ttcgtgaaaa accgggccat gtgcatgttc cagcaggcgg ttggggccat
     8401 ggccaccgca tcgcccgtcc agcccatccg ctcatcacgc tgcggacaat cggtccagac
     8461 gctgtacatg ttcgatcgct ggctccacgt gacgttccgc cagatctgat tgagaagcgg
     8521 gttcgagcat tcgaatctgc ccgccggcgc cgccgcggaa tggaggacac ggaacgccag
     8581 ggaatcgacc gtgggcttgc ccggataacc cgtgacttcg cagtaacgaa agccatggta
     8641 ggtgaatgtc ggttcccaga tttcctgccc ctcgcccttg aggatgtagt agtccgtggc
     8701 ctccgccgcc cgcaggttgt cacggtagag ctggccgtcc ggccggagga tctcggcgaa
     8761 ccggagccga atccatgtgc cccgggggcc gcggacactg agccggacgt atccggctgc
     8821 gttttggccg aagtcgaata cgtacacccc cgcagccggc tcggtgatct gcacggcccg
     8881 cagttcttcg gtcacccgta tgggttcaca ctgctcggcg acaagctttc cgccttgttc
     8941 cagctcgatc gcgggcaccc agtcgcgggc gatgaaggtt ggcgtggccc agccgggcat
     9001 ttcaagtcgt gcatcgtatg tctcgccatt aaaaaaggtg ttccgagtga tcggcgaagt
     9061 gtgcgtctgc caggttcgat cggtcacgac ccgctcgcgc gtgccgtcat cgtactcgat
     9121 ctcaagttgc aggatgaagc gcagcggtcc ttgcgagaat ggcggcttgc gggtccagcc
     9181 caggtcgccc gagaaccagc cgttgcccag gatcgctccg acggcgttct cgccctcgtg
     9241 caggagagcg gtcacgtcgt acacctggta ctgcactcgc ttgctatagt ccgtccagcc
     9301 cggagcaaac acgtcgtcgc cgacgcgatg gccattgacg taaagctcat acagacccag
     9361 cccgctcgcg caggcacgag ctctcacaac ccggcggctg agcgcgaaga ccttgcgaag
     9421 gcacaccgag cgcggcgggc cgaaaggatc ctggatctgc ttccagcggt cggccttgtc
     9481 agcgggcagc acgacggcgt gctgccatgt tgactggtca aagtccggct cctgccattt
     9541 cgtctgctcg gtggtattca ccagccaatc ggcatcggac gcgagatcaa acagtcgccc
     9601 gtcgacgaac cgaatccgca aagccgcccg caggccgccg ggatcaaccg agttgaccac
     9661 cttaacggcg atcacattga ggccgggccg cacatcgggc gtgacgtcgt actcgcgcgg
     9721 catgtcccag tagcggcagc cgccgacgaa tccgccgttg acgtagagct caaacgcatt
     9781 gtcggcgttg agcttgatga tagcttccgc gatcttcatc gaaggggcaa ggtcgaacga
     9841 acggcggaaa aaagcggtcg gtacctgggc cggggcatcg gggacccaga tccactgacc
     9901 aagttgcagc cctgcaccca gccctttcaa ttcatcgcaa gcgatccacc gggctgtcca
     9961 atcgctcggc ttgagcaggc ccatctccca ccaggccggc tcgctgtacg cggacacctg
    10021 gtcatcgcgg tcccagaccc gcaccttcca gtaaacccgc gcccccgaca acaacgaact
    10081 gccatcgtag accacgtgca cgttctggtc tgagctgacc ttgccgctgt cccagaagtc
    10141 gccctggtcg cgatcgagcc tctcgctgct gccggcggca aggatctggt aggcggtctg
    10201 cttttcatcc cggcgatctg cgtggagtcc ccagctcagc cgaggtcgag ccgcatcgat
    10261 cccaagtgga ttgacagcgt actcgcaacg cagtcgcaaa ggagaaagag cccccgtcgc
    10321 cgaagcagtc atcgtcgcaa gtgtgaccac tatagccaac atcaggactg caccagtgcc
    10381 cctcacgccc acattcgtcc accgtctctc caactttgcg gcagttcgcg gttgcgcaaa
    10441 atgccttcgc ctttcagaga cgtctccttg ccgccagcgt tcccgatcac attaacaagg
    10501 ggggacgcct cggccgacaa gccgggcctt agtctaccaa tcccaactcc gccaaacggg
    10561 cccgatcacc gagatggaaa cctgatttca gcagcttccg ttgaagctgc ttcacatcaa
    10621 cggcccgagg ctcgatgccg ctcttggccg ccatggccgc cgcggtaccg ccggcctggc
    10681 ccatgtacat caccccctcg cggttgcgca acagtgtgtc tggaatcccg gaggcactac
    10741 gtcccacggc gatcagtccg tccgtcttct tgggtatcat cacccggtaa ggcacgtccg
    10801 tccattcaac gcgcccgtgt tcgttcatcc agcggaacgc ccgcggttcg ccgtaacgat
    10861 agatgacgtc gtcgaagcgc ttgccctcgc gacagtcttg catcgtcagc gtgtactcgc
    10921 cctcgatgca cggcccgccg cgacaaccca ggtaaggagc ggtgaacaag agataagcat
    10981 cttcgcaccc gggcacattc tttcgacaat gctgtacgaa ctcgaagatg tacatgcgta
    11041 cgcctgcttc caacaacgaa atgtgctcgg cattgcccgc atcgaccttg ggatgaggcc
    11101 ggaccagttg ggccttgatg cccctcatgt gtctctcggg accgatctcc acgaggccgc
    11161 tgcatcccac ctgggccagg tcgccgatgt ccttcttcgc ctcgaactca gaagcctcca
    11221 tgcctttgaa tctggcaggg tcaatgcccg ccacgacagc ccagatgccc atgcctgttg
    11281 gcgaatgatg atcgagatca taagtttccg ccttcggctg caggatagga gtgcccgccc
    11341 ggcgagcgac atcggcctcg cccgtggcgt cgatcaccac cttggctttg accgcctgac
    11401 ggccggactt gttctctacg aacacaccgc aaaccttgtt gccttccatg atcgggtccg
    11461 ccacgtaggc ggacagcatc agtgtcaccc cggcctcctt cagcattttc agggccacat
    11521 aagcggcctt gttggagtcg gcgagggatc gttctggcgt gccagacccc aaagcgaaat
    11581 actcccggct aaactccttc ggcagcccgg ccaagccccc tttcaccgga tcaccggccg
    11641 gaccactggc cggggcgtcg ccgtgctcgg caatcggccc ctgctgagac atcatgcccg
    11701 gcccgaaact ccctccgaca tagccgaacc ggtcgatcag cacggtcgtc gcgccctccc
    11761 gcgccgcggc cagcgccgcg aaaacgcccg agatgccgcc accggccacg acaacatcga
    11821 cgtcatacgc aacgggcact tttttggcgg gctccttcac gaagcccaaa gaagattgtg
    11881 caccaggcga ggcgacctgc tgatccgcgg cctttgcccc ggtgagactc gccggcagag
    11941 cgaccagaac cagcgtccac ataaccttac aaaccattgc ccttgccatg atgaattctc
    12001 cacatcttga ctattcgtca ttgcatgcag gccgccctga cacgccgatc ccatcaattt
    12061 cgcacccaga tcgggctcga tatggccaga tcgccattgg cctgcacgat gcgagcgtag
    12121 taccagtcct cctttgcccc ctcgtccggc ccgtcagaga actcaagccg gcccgtgtag
    12181 tgcggcgcga agactaaccg ctgaaaaacc atcgattcgc tcgtaaacgg gccggtaaac
    12241 tcaagggtgt tctccgagga cagatccttg aggctcttct ctatcttcat ggcgacaggc
    12301 cgtgtcatcg tacacttgag aacggcttgg gggccgcctt cgatcttcag gaccacgctg
    12361 ttgttgacca gatcgccgag cggttccttg cgcgaggtgt aggagcggac ccgatacgac
    12421 ttctccgaca gcttctccac cctgttgcgc cggtcctcgt cgaacggccc ggcgcggaaa
    12481 caaccctggg cctcgacgat tcggccgccg atgatctcca attcgaaatc ccagtcacag
    12541 atgcgagtca tcgccagatc cccccagggc ccccagccga attcaacgcg gcatagcacc
    12601 ggcgacgacc aaggcggaga atgaacgtcg gcgtccgttg ggaaggcccg agcaaccaca
    12661 cggccgttct tgaccagctc aatcgacgca atctcatccc agccgctcgc cgtgatggtg
    12721 acctgccggt tcggggtctc aggcaggatg tcacccatcc aatggccgtt gacccggaag
    12781 tccaaggcaa tgcgatcgcc cgtgaccgcg aacgaccgcc gggcacgcag ggcctcgaaa
    12841 accccctccc tcgtcaactc gttcgcatag aggcccacca gcccctcacg atacgccccc
    12901 ggatagccac tgtgattgtc cgtgctggcg acaacgccga catgcgcgcc tcgggcaagg
    12961 agggctcgca gggtcctcgg tgtccaccgt ccgccgttgc tgtggcgaac gtagtcgaac
    13021 ggcccgcggt cgtgctcggc cagaccgtgc tcggagaaga tctccacgac cggagacacg
    13081 tccgtgtcaa gaaactcgaa tgccgtcccg cgccactgcc accgataagc cgggtgatgc
    13141 gggatcagca aggccccctt ctctttcgca aaggcctgga ccttcgatat gtgatcgaaa
    13201 tactccagcg gcgcgtcgac gtcggagggg tagatcaggc actgatcacc gaatcggctc
    13261 gagtgccatt cgtaagccac gaagggaacg aatcgccccg gcttgtagcg ctctttggcc
    13321 atccgccgaa cctcgtccca gtgttcccgc gtggccgcaa agccgttcac ccatttctcg
    13381 tgcttgttct cgggcatgat gggcatgtca tgccagtggc cgtgggcggt gaaggcaaag
    13441 aaatccaggt ggctctcggc gatctcgtag ctgcgctgca aggagccctt gccgtatcca
    13501 accgcattgt gattgtggat atcgccccag aaaagctgcc tgggctgatc ggccgtcaag
    13561 cggacattga tcagcaggac aacagatgca aacaccgaaa tgaacacacg ttgccgagaa
    13621 ggaatcatcg tgaaacctgc ctgttgcaca ccgcgcgatc ttatgccata atccgcatgc
    13681 gagtcatctc atccggcaag acgaggttgt tccggatgag cgagattcgc tccttcgcgg
    13741 ccccattata ccgggaagac gatgcttgtc cggcaaggca agcagcggaa ctcggcatga
    13801 gaagggactt gcgagagctg ctttctcccc acctgccccc agggagcgag cggttgacga
    13861 tgagcggatc atcactgcac tcggacccct gttgcggaag aagtcggacg gcatcggtac
    13921 gtcgtcgatg gctgcgctga gcgtggccag agatgcttct gtttgcgcat gtcgccacag
    13981 aagaacgctg gagatgcgcg ccgcggactc gaaccgcccc cccaaccggt gcgcacggag
    14041 aaagcagaaa ggacgtgagg cgatcaatgc gtgcaaatcg acggataggc acgatcctgc
    14101 tgttactggt gagcgcttgt gatcggggtg ccttggcagt tgatctctac gtggcgccga
    14161 gcgggaagat cgagtggtcg ggaacactgc cggcggccaa cgccgagcat tccgacggcc
    14221 ccctgccttc aatcgcagcc gcccgagacc tcatccgttc acgccgggcc agcgggatgt
    14281 ggacggacga ggccatcacc gtgcacctgc gcggcggcgt ttactacctg gaggaaacgg
    14341 tcgtcttcga acctcgggac tcgggaaccc cgggcaagcc gattgtctat gcagcgtaca
    14401 agaacgagca accgatcctc agcggcgggc gggtcatcgg cggttggcgc gacgcggaga
    14461 tcaacggccg accctgctgg gtggtcgaaa tccccgaggt caaggaaggc aaatggtatt
    14521 tccggcagtt gttcgttaac ggcagacgct gcccgcgtcc gcaggtgccg gaacaaggct
    14581 ggtaccagtt cgccggcatc ccgccacata gtgaaagata cttgaagaga gagaacgtgc
    14641 gcgacaaggc caatcagaat gaccgggcgt gttaccgccc ggggcacgtg aagaaatggc
    14701 acaatctcgg cgacgtggag ttcgtttgtc tgatgagctg ggacgaggct catctgttcg
    14761 ccgccaacct ggacgaggcg acccggacca tcgagttcac ccaaccaagc tacctgggcg
    14821 ccgacaaggt catcacagac gacaagaacg gacgctacta catgctcggc gtgcgagaag
    14881 ccctcaacaa acccggacag tggtacctcg atcgagtcag cggcatgctc tactacctgc
    14941 ccgtggccgg agagacgccg gacaacacaa cagttgttgc cccccggttg aacgagttgc
    15001 tgtgtgtgct cggcgacgaa ccccgcaatc cgcttgagga aagaaacaag acattctgga
    15061 cgggaccgcg gctgatcggc cagggaccca ctcccaggcc gattgagcat ctggccctga
    15121 gaggcctgac tttgaagcac aataactggt tcctgcctcc cgataagtcc gccggaggcc
    15181 aggccatggc ccggctgccc gcggccctgt tcctgctcaa cgcccggaat tgccggatca
    15241 ccgattgcac ggtctcccag atctccaatt gggcgatcga gctgggcgcc gaatgcagag
    15301 acaacgtgat ctccggctgc accctgaccg acctcggcgc cggcggcgtc aaactcaacg
    15361 aggacagcca tcacaacacc gtcaccgatt gtgagatttc caacggcgga ctggtgttcc
    15421 ccggcgccgc cggcgtctgg gtcggccaca gttgggccaa caccgtttcg cacaaccata
    15481 tccatcatct gtactacacc gggatcagtg tcggctgggt ctggggatac tcccggccca
    15541 gcaaggcggc ctgcaacatc atcgagtaca accacatcca ccacatcggc cggaagctgc
    15601 tcagcgacat gggcggcatc tacacgctcg gctcctctcc gggaaccgtt ctgcgctaca
    15661 atctcattca cgatgtcgag gccttcgcgt acggcggcca cggcctgtac aacgacgagg
    15721 gcagcagctt cgtccttttg gagaataaca tcgtctaccg gaccagccac gagtcgtatc
    15781 atcagaacta cgggcaatac aacgtgatcc gaaacaacgt gttcgcctat agcgccgacg
    15841 gcacggtcaa ccgatcgggg cagaatgacc ccttctcgtt caccatcgag cacaacatcg
    15901 tctacagcac gttgccgcgg atgctggaca acggatggcg ctggaacaag agcggaaact
    15961 tcaagatgga ctacaacctc tactggcggg aagacggtca gccgttcgat ttccccggcg
    16021 atcgatcatt cgaacagtgg cagcaatggt gggaccagga caggcactcg atcatcgcgg
    16081 atcctctgtt cgtggaccca aagaacggtg acttcagact gaaggccggc tcaccggccg
    16141 agaggatcgg cttcaagccg atcgacacga gcaggatcgg acgactgaag tcccatacgc
    16201 cgggataatg gcgtccgagc gagtctccgc ctgtgacgga acgcggcgag aggaggacta
    16261 ctgatgacaa gcggctctcc agtctcaagc tcccgactcc ccggcatcca ggcgaccgcc
    16321 ctgtttgtgc tccgtctggc gattggatga cacttcctat gagaagtaat tgcaaggtac
    16381 gtgatccgaa cgggacgtcc gcgggtgacc tccttgaatc ccatcgggtc ctttcggact
    16441 tctttcattg gatcgtcgcc catccgcggg tgctgagggt ggttgacctg ttgaacatct
    16501 ggggcttgat cggcatcggc ctggccccgt tcttaaaccc tttgacacgc ttggcgtgtg
    16561 cgttcggcat cctgctcctg gtcctgtact acgtgtcctt ccggtaagcc tgcatgaaac
    16621 ggcgtgaggc ggctggtatc gtcggccgtc gccgcgaggc ggcagggagg acggcggatg
    16681 agaaccggaa cgacgcgatc gcccgcgagc aaaacctgtc gccggagcaa cggctggcct
    16741 tccaccaggc cgagagcggc ccgatcatgg agcagctccg ggcctggtgc atccggcagt
    16801 tcgatgaacg gctggtggag cccaactcag cgctcggcca ggccatctcc tacctgctca
    16861 agcactggga gaagctgacc ctcttcctgc gtcagcccgg agcccctctg gacaacaaca
    16921 ttgtcgaacg agccctgaaa cgagcgatcc tgcatcgaaa gaacgcgctg ttctacagaa
    16981 ccctcaacgg ggcccgcgtc ggcgacctgt tcatgagcct catccacacc tgccagctca
    17041 acgacgtcga ccccttcgac tacctgaccg aactgcaacg ccacgccgaa gacctcgccg
    17101 cccaacccga aaactggatg ccctggaact accgcaagac aatccaggag accgacgccg
    17161 acatccctgg ctgatccctc ggttactccg tcccaccccg ggcacgccgt ccaagattac
    17221 gcatgcttac cggaaggaca cagcgcagct acgtccacgg catgcagtat attgacgagc
    17281 gggcggtgat gagggatcat caggcggacg caaagccggg cgacaaccag atgggcaagg
    17341 actactacta cctgctccag gagttgtaca ccgtggccgg cctggccgac tgccggggat
    17401 ggatggagga gatgtacgtg tacgacacgt acgggggggc gactctgtac gactggtggc
    17461 cgggagacgt caaccgcaac ggtcctgtca ccgccggcga catggcactc gtgtccgcga
    17521 gggtcggtgc tggctgggat gaaccctggt gtgatgccaa cctggacggc gatgtaacgg
    17581 cagccgatgt gtcggagatc gctgcattga tccccgtttc atcttcacct cctcccacga
    17641 tcaactacag ttccgtcgac aatccctatt tcttcaccgg ccgtaccacc gacacgctcc
    17701 acgcctccgc cctgctggtc agcgaagacc ccgacttcaa acgaatccag gacaaccgca
    17761 accgaatgta cgaccccaaa cacggccgct ggctgcagag ggatccggcg gggtatgtgg
    17821 atggggtgaa tctgtactcc tatgcccgga gcaacccgac gggtggtctg gatcccttgg
    17881 gcacctggga ctaccgtgtt cacacagaaa agacgaggag ctgggcgatt gacgaggcga
    17941 tgacggaaac cggcgcagca cgcgtggcgc attatgatga ggctaccgac acatttgcct
    18001 atttcggcga atacgccgag cagtcttgga cgccggtgcc gggcggtcgc agccagggtt
    18061 atcacttcaa cgcctacagg tacggtgttg attcaaggca tcagcatcga gatgaggatt
    18121 acgacaaggc gctcaaggca tgccttgaca aggatgaccc tgagactgcg tcaaggtggc
    18181 tggggcacag tctccacgca cttcaggatt ggtatgcgca tggcgagtac aattcagggg
    18241 attctgacac cttcatcacg catgggccct actacgatga tcccgagtat gatgccgccg
    18301 gccctgtcta tcgagtctat catgtcacgt tcgaaagctc ctacaactgg cccggcgatg
    18361 acgggtacaa gataccgcca gcctacccgt tgtactggtt ctataaatac gacgcgttcg
    18421 acaggccaaa tggctcgact ccggtccagg cacgttctga cgggcggccg ccaaaagtct
    18481 acgggcgctg gctgacaaga cttcaaagcg gttacggtgg gcgcgccggc gagccttctt
    18541 atctttcagt gttctgggag gccaggtggc tgttgtggga aagggggaac aagcgagtaa
    18601 gcggaacaga gatggaatct cgatctcata tcgacaagtt cttgcgggca atctgcacag
    18661 gctgcaacaa cggcaagtgt ccgaagtgcc agtgccattt tatccaattt ggccccctcg
    18721 ccacaagttc tgctggaagt agtcgtcgtt gcggggagga atgtgcggcg gaaagtgaag
    18781 gaaccgaacg gtgaggacga gaaccagact cagactgttg cgctgtgccg tgatccttat
    18841 gccgcttggc cttgtgattc ctggcgtggc cgcaatcatc ggcaagccgg tgggctctat
    18901 gtggcttacg aactattcct gtccccttgc gtttctgctt gccgacgcga tcatattagc
    18961 ctccagccgt gagtggacct tgagggtctt cgccttcctg tccatcgcct cgcttgcggg
    19021 ggcgatactc tgcgtcaatc ttcctggcat caactacaat atgatgaacg ggcctgggta
    19081 tttctactcc gtgttgttcg cctttgtcct gctcccattg tccgcagtac tgtcactcgt
    19141 tgtcttcgtc cttgctctcc gggaagcacg ccgtgatccc gcgcctgagg ggcactgcac
    19201 tttctgcggt gacagtctca gaggctcagg tgacacacgt tgcccggaat gcggcgcgcc
    19261 cttcggttca tcgctgatgg gtggaccaga cgctggtgaa aagatcggaa gaaacagaat
    19321 aaggaatcca tcattgccga ctgggcaagg cgcaggtgac caatgaacga acgatcgagt
    19381 aacaagccgg taccgctgtt ggccctggcg gccgccctgg tcatccccgg cttcctgccc
    19441 caatccgccc acggcatgta cgaccccaaa cacggccgct ggctccagag ggatccggcg
    19501 gggtatgtgg atgggatgaa tctgtaccag tacacgggga gcagccccgt tgtgctggcg
    19561 gatccgacgg gagggtgctc caaacctccg ccgccgccat gtacgtgttg ctgtgcgcta
    19621 gcggaccttt ccgacttatc ctaccgcttc tcatatttct gaacaggctg g
//
LOCUS       DAOTPW010000097        19369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2885_length_19369_cov_5.512374, whole genome shotgun sequence.
ACCESSION   DAOTPW010000097 DAOTPW010000000
VERSION     DAOTPW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19369
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2885_length_19369_cov_5.512374"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..568
                     /locus_tag="P5316_10440"
     CDS             287..568
                     /locus_tag="P5316_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRR85417.1"
                     /translation="MNIYVGNLSFKTTEEAVRELFAAYGQVDRVSLISDRHTGRPRGF
                     GFVEMADNTAAQAAIEALNGKEVDGRKLKVNEAQPKAERSGGGGGGRGR"
     gene            complement(647..1570)
                     /gene="miaA"
                     /locus_tag="P5316_10445"
     CDS             complement(647..1570)
                     /gene="miaA"
                     /locus_tag="P5316_10445"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRR85418.1"
                     /translation="MNLLIGCTASGKGRIGLELARRLGGEIVSVDSMKIYRRMDIGTA
                     KPSEAARREIRHHLIDVVEPSEPYSLARYIEGADRAIDDIAGRGKPVLAVGGTMLYVR
                     GLTAGVFAGPGADLEFRRAFRERAAREGTCALHAELARVDPKAAARIHVNDLRRIERA
                     LEVYHLTGSPISELQQQWDRPMGRYDCRIAALRRPKEQANRRINARVHRMIEAGLVDE
                     VKRLLAEPGGIGHQAAQAVGYAEIIAYLRGRWNLADAVERIKINSRHLAKHQRTWMRR
                     IPGIRWVDLTEDDTVEEVADRVLEAWSQPAA"
     gene            2190..2837
                     /locus_tag="P5316_10450"
     CDS             2190..2837
                     /locus_tag="P5316_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85419.1"
                     /translation="MKRSFLAAVAFVFVAASFANATSIPLGSSGWQVVWDSGLNPYLN
                     ITVEGEDADAVYIHKTVEFIQGPDEFGNFPTIPITFWQIGPSTITKIVILDETIKNST
                     GANWTDFHWDLIDHGEAWFIHDPGWFFKTSPLDNQSWARDDMNFSVDGFATNGVVYNG
                     SVWQPGIGVDGGELVIHVTSKPAAPYTLFTLKETPTPEPASLLLLVLGAMMLRRR"
     gene            3436..4107
                     /locus_tag="P5316_10455"
     CDS             3436..4107
                     /locus_tag="P5316_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85420.1"
                     /translation="MKGMFSAAAAVTLLVVSSANAGLVVLDNTGWQAVWDDGLDGLVD
                     IWIPPASPPTASIIFIQKSAQFTQGPDEFGQFPSIPITFMQTGPSTITRIVIQDEIIT
                     NTTGVDWTDFHFDLLDKGDAWFEHGPGFFFTTSPLDHQVFSADNKSFSVDGFGLGPGG
                     TDAVVPNNSVWFPGDGPTDGNLVIRVVSKTSEPYTVFTLKETPTPEPASILLLALGAV
                     LLRRR"
     gene            complement(4235..5167)
                     /locus_tag="P5316_10460"
     CDS             complement(4235..5167)
                     /locus_tag="P5316_10460"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRR85421.1"
                     /translation="MANDIIEWMNLDTLRVFCDVVRCRSFSRGSALNHISQSAASQAV
                     HQIERRLGMPLIDRTKRPFILTPEGQVYYDGVRQVLDRYDAVESQVRSLRNEVAGQVR
                     VAAIYSVGLHDMSQAMQTFMRQYPKAKVRLEFLHPNKVYDAVLNEQVDLGIVSYPAAI
                     RGLTVIPLRTENMALVCSPNHRLANMKRVTITQLRGENFIGFDRELIIRRELDRYLRE
                     NQVLVNMVMEFDNIETIKQAVEIGAGVSILPEPTIRKEIQTGSLVAVPLASQKIRRPI
                     GIIHRQRKIFTPAIVRLIELLKSMKSETSEPRRV"
     gene            5392..6732
                     /gene="gdhA"
                     /locus_tag="P5316_10465"
     CDS             5392..6732
                     /gene="gdhA"
                     /locus_tag="P5316_10465"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRR85422.1"
                     /translation="MSYVQNVLETVIKRNPGEPEFHQAVREVLESLQPVFDRHPKYEK
                     HRILERFCEPERVIIFRVPWLDDKGNIQVNRGYRIEFNSAIGPYKGGLRFHPTVYLGI
                     LKFLAFEQILKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELCRHIGPHTD
                     VPAGDIGVGGREIGYLFGQYKRIRNEFTGVLTGKALNWGGSLIRPEATGYGAVYFAEE
                     MLATRNDSMRGKICAVSGSGNVAQYTVEKVNQLGGKCVTLSDSNGTIYDPAGIDAEKL
                     AWVMELKNVRRGRIREYAEKFKGVEYKDGVRPWGVKCDCAFPSATQNEISGEDAKTLV
                     KNGCKLVCEGANMPTEPEGIEVFLTNKILYGPGKAANAGGVATSGLEMSQNAQHTNWT
                     REEVDRRLHDIMIAIHKAVSKTAAEYGEPTNYVMGANIAGFVKVADAMIDQGVV"
     gene            complement(7018..7740)
                     /locus_tag="P5316_10470"
     CDS             complement(7018..7740)
                     /locus_tag="P5316_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR85423.1"
                     /translation="MYKGKKIIAMAPAHNEEAKIGQVVARTDRKLVDTILVIDDASTD
                     RTVEVAREGGATVLSTGHRMGVGGALRMGIEYGRQTGHDVAVIMAGNNKDNPAEIPRL
                     LDPICDQNIDFVIGSRFLVGGAYGGDMPGYRKLATRLHPWLLSLLVGKKLTESTNGFR
                     AFRLSLLDDRRINLNQSWLDGYGLEVYLLFKTIKLGYRHAEVPCTKIYPPRRLGYTKM
                     KPVVGWWSILRPLFLLGLGVKQ"
     gene            complement(7745..8350)
                     /locus_tag="P5316_10475"
     CDS             complement(7745..8350)
                     /locus_tag="P5316_10475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRR85424.1"
                     /translation="MPASSVSAGPFVHPSALCESPDVGEGTRIWAFAHVLKGAVVGRD
                     CNIGDHAFIEGGAVVGDRVTIKNSALIWDGVTIEDDAFIGPGVVFTNDLYPRSPRMPG
                     ARARYAHPDNWRVPTRVCRGASIGAGAVILCGVTIGSFAAVGAGTVVTRNVPDHRLVV
                     GNPARPAGWMCRCGMRLDESLSCPRCARRFVLRDDGLEEAD"
     gene            complement(8470..9531)
                     /locus_tag="P5316_10480"
     CDS             complement(8470..9531)
                     /locus_tag="P5316_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85425.1"
                     /translation="MAADGAQVRLAVIGCGRWGPNHIRVFSESDRATVVGCADISQQR
                     RENMLKRFPQIRAVADYHELLADESIDAVVIATPTFTHAQIAREALQAGKHVLAEKPI
                     CATTREAAGIVRLAEDTGLVFMVGHVFLFNNGITELRKLVESGELGRIHYMEANRTNL
                     GPIRGDVNALYDLATHDISIFNYLIGCSPVEVSAVGRCISQKNIEDVCFATLKYPDGT
                     LGHIHVSWLNPRKVRTMTVVGDRQMAYWDDIDPDNTLRLYDKGIDQEPHYDSFGEFRY
                     LLRSGDMHVPAIRQSEPLVNQAQAFLDWVLGGKRSLSDARYGLEVVAVLEAATRSMAA
                     GGTMCRVEAEVPEALCEVA"
     gene            complement(9663..11477)
                     /locus_tag="P5316_10485"
     CDS             complement(9663..11477)
                     /locus_tag="P5316_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85426.1"
                     /translation="MTTPEDQPGQLPPAVTTGPDSPWFRRGLLLCCWLVAVGLGAIHA
                     WGDRHTMNPDGISYLDLAEAVRAGRWSDAVSGYWSPVYSCLLAVGLMFSGASPYWEAT
                     VAHAVNFAVYLCALASFHFLWVAILDRGRSRARDAAADAGEQLPSWAWFVLGYALFIW
                     TSLDLITLRIVNPDMCVAVCVYLAAGIVVRLQGGQAGIGWFVALGLVLSIGYLTKAIM
                     LPMAVVFVIVALLAAGQFRRLPARVVVVLICFALPAGPYVAFLSVQKGRLTFGDSGRI
                     NYAAHVSGIYPWDHWRGLPPGTGTPVHPSRQILDKPAVFEFAGPVPGTYPLWYDPSYW
                     YDGVKPTFNLSRQARALWRNKDAYFDLLCRQQGAWLFPVLVLFAFGSRLGSALREISS
                     YWPLLLPALGGLGIYALVHVEPRYVAPFVLLGWGALLPAVRLPAGNLSGSLIRAVCGV
                     SLLALLLTIGTEACPAISRTVSDLRLRTDTAPHPQWQIARQLGEMGLRPGDKVAHIGQ
                     ALKSWSYWARLAGVQIISELRPEESFWLSDDATRGRVIEAFRSTGARAVIARRPTSDE
                     RPWGLPIDFAPGFQPVGNTGHHVLFLVTPATQTASAPG"
     gene            11633..12739
                     /locus_tag="P5316_10490"
     CDS             11633..12739
                     /locus_tag="P5316_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRR85427.1"
                     /translation="MSLPMPTSEVLAAIRSGCVIPAHPLALNAKRTLDERRQRALTRY
                     YLAAGAGGVAVGVHTTQFAIRHPSIGLLRPVLELASEEVNRFTRTGGKSAIIKIAGIC
                     GETRQAVEEAQLARELGYDIGLLSLSAMKDRSLEEMVRHARAVAEVLPIMGFYLQPAV
                     GGRVLPFAFWRRLAEIDNLVAIKIAPFNRYQTLDVVRAVAESGRADQVALYTGNDDNI
                     IADLLTACRMNHGGRQVTLRIVGGLLGHWAVWTRRAVEQLDAIRRLIAGHPEPAGSPY
                     AGMTIADWLRQGIEVTDANAALFDAANSFAGCIAGLHEVLRRQGLLEGRWCIDPAEDL
                     SPGQAEEIDRVYAAYPHLNDDAFVAAHRDKWLSG"
     gene            12758..13360
                     /gene="arfB"
                     /locus_tag="P5316_10495"
     CDS             12758..13360
                     /gene="arfB"
                     /locus_tag="P5316_10495"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRR85428.1"
                     /translation="MTAGMWGNCSDEASLQAWFMLPLAAPLASGSVLPYNMGVLASFS
                     CDAINERVTERAAIVIQISSGVGISECELRFSFDRSPGPGGQNVNKVNTRVTLEFDVD
                     RSMSLTVAQRTQIRRALASRIGQDGILRVVSSKERTQLGNRRAAVNRFIELLGAAFHA
                     PKPRRKTSPPASANRQRLEVKAKRSQLKRSRSAEIAVGEE"
     gene            complement(13481..14449)
                     /locus_tag="P5316_10500"
     CDS             complement(13481..14449)
                     /locus_tag="P5316_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HRR85429.1"
                     /translation="MKAPWITYRPELKVLDCTIRDGGLINDHQWDEKFVKAVYQTCVE
                     AGIDYMEIGYKASKKLFARDKFGDWKFCDEDDLRRVVGDNETDVKLAVMADADKCDYK
                     EDILPKDESVIDVVRVATYVHQIPTAVAMINDAYDKGYEVTCNLMAVSTVQEPDLDQA
                     LEVLAASPASTIVVVDSFGSLYAEQIEILVKKYKSYLNGTGKEVGIHAHNNQQLAFAN
                     TIEAIIHGANRIDATMGGLGRGAGNCPMELLIGFLRNPKFRLRPILKLLQDHVEPMRK
                     AVEWGPMVPYIITGQLNQHPRSAIKMLSGNGRNNFVEFYDTCVADE"
     gene            14621..14693
                     /locus_tag="P5316_10505"
     tRNA            14621..14693
                     /locus_tag="P5316_10505"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14654..14656,aa:Ala,seq:cgc)
     gene            complement(15091..15164)
                     /locus_tag="P5316_10510"
     tRNA            complement(15091..15164)
                     /locus_tag="P5316_10510"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15128..15130),aa:Val,seq:tac)
     gene            complement(15214..15993)
                     /locus_tag="P5316_10515"
     CDS             complement(15214..15993)
                     /locus_tag="P5316_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85430.1"
                     /translation="MRTVLRPDNPFGPGRHGFAWEHVPSGRRCHLDFGCGSGRFLETL
                     RDKGFQRLIGIDASREAIEAGRRERPGLELIHRREPLPLPFADREIDSISLLDVLEHV
                     VNQKGLLAELHRVLKDDGVLIVTVPGLHVFSCLDLGNLKFRFPRLHRWFYRLRHSQED
                     YEHRYAANPDGLIGDISAEKRWHEHFSREDLAGLLSGAGFEVSLFDGSGFFMRLLAPF
                     HHLTRPVGPVNRMFQKLQRGDHLRYESANLFCVARKRPVTG"
     gene            complement(16115..17368)
                     /locus_tag="P5316_10520"
     CDS             complement(16115..17368)
                     /locus_tag="P5316_10520"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRR85431.1"
                     /translation="MALQVKKVVLAYSGGLDTSVILPWIKETYNCEVIAFAAELGQGD
                     ELKNLRKKALDSGASEYVERDLREEFVKDFLWPMLRSGAVYEGKYLLGTSIARPLIAK
                     EQVKVAHEYGADAVAHGATGKGNDQVRFELTYMALDPSLKVIAPWKDPRFELRSREAA
                     VAYAEARGIPIEQSKKKIYSRDRNFWHISHEGAELEGTWAEPQDRMLVLSVPIEKAPN
                     TPAYVEVNFEQGTPVGLNGKRMDGISILTRLNELGGKHGVGQVDLVENRLVGMKSRGV
                     YETPGGTILYEARRALEGICLDRETLHYKEIVSHRYAELVYYGQWYSPLRHALDAFFT
                     QVNKPLTGTARIKLYKGNCRAVGTKSPRSLFIPALASFTMGAEYDSTDAIGFIRLFGL
                     PMKVNGIVRRQAARASRARAARKKK"
     gene            complement(17534..17974)
                     /locus_tag="P5316_10525"
     CDS             complement(17534..17974)
                     /locus_tag="P5316_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85432.1"
                     /translation="MWPFGWWRRFRRRIKLLQASGASIDLAELKELAESSPTKKYVNL
                     LLLEMAKGSIDELTLSRSMPLPRSKLDPNAPIPTFESMVNRLKVMAALDPVIYQHPKE
                     GRIELTIGSDPLVVHVRFVDTAPERSVHLRLEWLDEPPPPARLR"
     gene            complement(18022..18792)
                     /locus_tag="P5316_10530"
     CDS             complement(18022..18792)
                     /locus_tag="P5316_10530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85433.1"
                     /translation="MMETDDKQRFAHMAEDYDQVAPFLVPMYDFIQQEMIRLADLAAM
                     PRPRIVDLGAGGGRFLERVLAVCPQATCYWVDSSESFLAVARRRLARFGEQINYILSP
                     MEGDWTRQIDHPVNCVFSMSAIHHLERHEKKALYARCFGLLAEGGWFINGDEMKTLDE
                     NAYRSSLDFWVRHVQGRADHIPPALVENARRWCGHFDGWKRRNIERIDQPKTKGDDLH
                     EPFIDQMRWLKDTGFAGVDIFVKYHLWCVIGGRKPIQA"
     gene            complement(18835..>19369)
                     /locus_tag="P5316_10535"
     CDS             complement(18835..>19369)
                     /locus_tag="P5316_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRR85434.1"
                     /translation="GACNTDYRGAMDALCAGICCTREELGRLSVAVLGAGGVSRAIVA
                     GLRDCGCRVTIYNRTFERARTLAAEFGAEARPLNQAADHEADIIINGTSIGMWPNVDD
                     TPLPADALLGKPVVFDTIYNPIETRLLREARLRGCRTVDGVSMFVNQAAAQFERWIGR
                     PAPVDILRDVLLRRLGR"
BASE COUNT         3864 a         5854 c         5829 g         3822 t
ORIGIN      
        1 cctgaagata tcagtttcgg agccgcgagc accgatctcg ccggcggggc ccgccgagat
       61 tcgggaaccg cttgcgggcg ggctccagga cgtcggcacg gagtcccgct gcgtcggtgg
      121 agatcgaagg tctggctaca tcgttgcctt tcgtctcttg catcccagcc gcaatcagag
      181 tattcgtaat cgtgctacca aatggtgttg tcactgaacc atgcgttttt gcccaccgag
      241 cgtccgggcg gcaggttcag aagccaaggt gaggagttgc tccaccatga acatctatgt
      301 cggcaatctc agcttcaaga ccacggagga ggcagtacgt gagttgtttg ccgcttacgg
      361 gcaagtggac cgggtcagcc tgatcagcga ccgccatacg ggccgacctc gcggttttgg
      421 cttcgtcgag atggccgaca acaccgctgc ccaagcggcc atcgaggcac tcaacggcaa
      481 ggaggtcgac ggtcgcaagc tgaaggtcaa cgaggcccaa cccaaggccg agcgatctgg
      541 cggcggtggc ggtggtcgtg gtcggtgatc caatgggaat caccggcaaa gttcttcgag
      601 cttggtagca cgacgggaga tgaagagccg tccgagcttc tggttgtcag gcggcgggtt
      661 ggctccatgc ctccagcacg cggtcggcta cctcttcgac cgtatcatcc tcagtcaaat
      721 ccacccacct gataccgggt atccggcgca tccaggttcg ttgatgcttg gccagatgcc
      781 gggagttgat cttgatccgc tcgacggcat cggccagatt ccagcggcct cgcaaataag
      841 cgatgatctc ggcatagccg acggcttgcg cagcctggtg tccgattcca ccgggctcgg
      901 ccagcagccg cttgacctcg tccacgagcc ccgcctcgat catccgatga actcgagcgt
      961 tgatgcggcg gttcgcctgc tccttagggc gacgaagggc ggcgatacgg cagtcatagc
     1021 ggcccatggg ccggtcccat tgttgctgta attcgctgat cggagagccg gtgaggtgat
     1081 agacctccag ggctcgctcg attcgacgga ggtcgttgac gtgaattcgt gcggccgctt
     1141 tgggatccac ccgggccaat tcagcgtgca gagcgcacgt cccctcgcgg gcagcccgct
     1201 cgcgaaaagc ccgtctgaac tcgaggtcgg ccccgggccc tgcgaaaacc ccggccgtaa
     1261 gtccccggac atacagcatc gttccaccca ctgccaggac cggtttgccc ctacccgcga
     1321 tgtcgtcaat agcccggtct gcgccctcga tgtaccgagc cagactgtac ggctcgctgg
     1381 gctcaaccac atcgatcaag tggtgtcgaa tctcacgtcg ggctgcctca gaaggtttgg
     1441 ctgtgccgat gtccatgcgg cggtaaatct tcatggagtc caccgagacg atctcgccgc
     1501 ccagacgccg agctagctcc agaccgatgc ggcctttgcc gctggccgta cagccgatga
     1561 ggaggttcac tcgaatctgc gatctttgac ccgaacttgt agtcatgccg agtgcagctt
     1621 agccaaggca ggtcggaacg aacaagcaga ccccaggcaa agagccaccc tcaacctgcc
     1681 gagccgccga caacccagcc gcaaagaatg gccttggcga gcctggcttt cccggtcagc
     1741 gtcgggtcgc tcaccggcca ggatcccgac ggacgaccaa cctgagttcc gcccccggcc
     1801 gcgatgaggc ccgggttctg ccgcaagcct ctcttatgcc ttgcgtggaa taaatttatt
     1861 ttatacataa tcgtcagcat ctccgttgac acggcctgcg ggtttgccga ggggaccgaa
     1921 acagcccaga ttaactgtcg cggaaatcca ggaaaaactc ttgctttgag gagggctatc
     1981 cggtagtatc cataatgtga gcgagagaat cgcttcacgt caagcgtcca gtgtcgaggt
     2041 gagcgaaagg ggcacaagta tcgaggttca tattctcatc ggctctgttg tgtggaagac
     2101 gcgcggtacg agacgcgcga tgatggggat caaacaccct catatctgat gcccatgtcg
     2161 agcgaggcgg aaaaagatag gaggaaagta tgaaaaggag ctttctggca gcggtggcgt
     2221 tcgtgttcgt ggcagcgtcg tttgcgaatg cgaccagcat acctctgggc agctcggggt
     2281 ggcaggtcgt ctgggacagc ggactcaacc cgtatcttaa tatcacggtt gagggggagg
     2341 acgcagacgc cgtctacatt cacaagacgg tcgagttcat tcaaggtcct gacgagttcg
     2401 gtaattttcc cacgattcct attactttct ggcagatcgg cccatcgacc attaccaaga
     2461 tcgtgatact cgacgagacc atcaagaact cgacgggtgc caattggact gatttccatt
     2521 gggatctgat agaccatgga gaggcgtggt tcattcatga tccaggctgg ttcttcaaaa
     2581 cctcgccgct ggataaccag tcttgggcca gggatgatat gaacttctcg gtcgatggtt
     2641 tcgctactaa tggagtcgtg tacaacggaa gcgtgtggca gccgggaatc ggggtcgatg
     2701 gcggcgaact cgtgatccac gtcacttcca agccggccgc accctacacc cttttcacgc
     2761 tgaaggagac gccaacgcct gagccggcct ccctcttgct gcttgttctt ggtgccatga
     2821 tgttgcgacg ccggtgagcg cattcctgct ccatttgagc ctgaagatca gtgccccgcc
     2881 cccgtggcgg ggcacgcttt ttctggggcg tccttccccc ctttttttgc accagccgtt
     2941 gcgggagtcg gatttgcctg tgggttttgg cccacattcc tcactccaga ggcccggacg
     3001 gtgggcaatg ggccccgata agttgtccaa tgctagcagc gctgccaatg gttccaaatt
     3061 gacgtaaacc tcgttgctga aaagtcttag ggtgattctc ttgcttttgt gttttcactt
     3121 gcctggcagg ctggttggct catgtcttca gacgggctga tccggggaat ggcaactaag
     3181 aaaaaatcgc caagaaaaca aaaaaacctt gacacgccaa cagtgaccgg ctaggctact
     3241 atataaggag agagggggag agctgcacat cgacggtgta gccctcatcg tgtcagttca
     3301 gagaaacggg aaaacgcgag caggcgcgca ctgcacatct gtacgaagtc gcgcttgcga
     3361 ggatgcgtgt ccgacgagcc ggcccattca aagatcgtcc cacacgcatc agaaaaggaa
     3421 aggcaggagg caactatgaa aggtatgttc tcagcagcag ccgcggtgac tctcttggtc
     3481 gtgtcgtccg ctaacgcggg cttggtggtg ctggacaata cgggatggca ggccgtttgg
     3541 gacgatggct tggatggttt ggtggacatc tggattcctc ccgcgtcgcc gccgacggca
     3601 agcatcatct tcatccagaa gtcagcccaa ttcactcagg gccccgatga gtttggtcaa
     3661 ttcccctcga tcccgatcac gttcatgcag accggcccat cgacgatcac ccgtatcgtt
     3721 attcaggatg agattatcac caataccacc ggcgtcgact ggactgattt ccatttcgac
     3781 ttgctggata agggtgacgc ttggttcgag catggccccg gtttcttctt caccacgtcg
     3841 ccgttggacc accaggtctt ctcggcagac aacaaaagct ttagcgtcga tggctttggc
     3901 ctcggtcccg gtggtaccga tgccgtcgtg ccgaacaaca gcgtatggtt cccgggcgac
     3961 gggcccacgg acggcaatct ggtgatccgc gtagtctcga agacgagcga gccgtatacg
     4021 gtcttcacgc ttaaggaaac gccgacaccc gagccggcgt cgatactgct gttggctttg
     4081 ggtgccgtgc tgctgcggcg tcgctgacca gaatgtcgga aagcgatgaa ttccactgcc
     4141 tttttcagat gttaacatgc cctgcgccgc cgcagggcat tttcttgccc gccgcgccca
     4201 ttctcgggac agtagcgcgt gcggtgacgg ggcactatac ccgccttggc tccgacgtct
     4261 ctgatttcat cgacttgagc agctcaatca gcctgacaat cgcgggcgtg aagatctttc
     4321 gctggcgatg gataatgccg attggccggc gaatcttctg actggccagc ggaacggcca
     4381 ccaatgagcc cgtctgaatc tcttttctga tcgtcggctc cggcaaaatg ctgaccccgg
     4441 ccccgatttc gacagcctgt ttgatggtct cgatattgtc gaactccatc accatgttga
     4501 ccaggacttg gttctcgcga agataacgat caagctcgcg acgaatgatc agctcgcggt
     4561 cgaagccgat gaagttctcg ccccgaagct gggtgatggt cacccgtttc atgttcgcca
     4621 gacggtggtt cgggctgcag accagggcca tgttctccgt tcgcagggga atgaccgtca
     4681 ggccgcgtat cgccgcgggg taggaaacga tgcccagatc cacctgctcg ttcaacactg
     4741 catcataaac tttgttggga tgcaggaact cgaggcgaac cttggccttg gggtattgcc
     4801 gcataaaggt ctgcatggcc tgactcatgt cgtgcaagcc gaccgagtaa atggccgcca
     4861 cgcgcacttg cccggccact tcattgcgca gggagcgaac ctgagattcc accgcgtcat
     4921 atcggtccag cacctggcgg acgccgtcgt agtacacttg gccttccggg gtaaggatga
     4981 aagggcgttt ggtgcgatcg atcagcggca tccccaggcg tcgctcaatc tggtggacgg
     5041 cctggctggc ggccgactgg gaaatgtggt tcaaggctga accgcgggag aaactccggc
     5101 agcggacaac atcacagaag acgcgcagcg tgtcgagatt catccactca atgatatcat
     5161 tagccaatct aatgtcaagt attagccgcg aaccgtttcc ataatagtac gggaatgtta
     5221 gactttctcc aatcgcctat tgacgccaag gcggcggcct ctgcacaccc ggccacaaaa
     5281 gggtttgtgc tcgcgaaagg ccgacgggaa cgttcccgct tgccggattc gcggggaatc
     5341 cgttaggcaa ctgcgccgga cgcagtatgc aacagaagga gagcaggacg catgtcctac
     5401 gttcagaacg tgctggaaac agtcatcaaa cgaaatcctg gcgagcccga gtttcatcag
     5461 gcggtcaggg aggttctcga gtcgttgcag ccggtctttg accgtcatcc caagtacgag
     5521 aaacaccgga ttcttgaacg attctgtgaa cccgaacggg tgatcatatt ccgcgtaccg
     5581 tggctggacg ataagggcaa cattcaggtc aaccgcggct atcgcatcga gttcaacagc
     5641 gcaatcgggc cctataaggg gggcctgcgg ttccatccga cggtttacct gggcattctg
     5701 aagttcctgg cctttgagca gatcctgaag aactcgctga ctacacttcc gatgggcggc
     5761 ggcaagggcg gctcggactt cgatccgaag ggcaagtccg acaacgaggt catgcggttc
     5821 tgccagtcgt tcatgaccga gttgtgcagg cacatcggcc cgcacacgga cgttcccgcc
     5881 ggtgacatcg gcgtcggggg ccgcgagatc ggctatctct tcggccaata caagcgcatc
     5941 cgcaacgagt tcaccggagt gctgaccggc aaggccctga attggggcgg ttcgttgatc
     6001 cgacccgagg cgaccggcta tggcgcggtc tatttcgccg aagagatgct tgccacgaga
     6061 aacgactcga tgcgtgggaa aatctgtgcc gtgtccggct caggcaacgt cgcccaatac
     6121 acggtcgaga aggtcaacca gctcggcggc aaatgcgtga ctttatcgga ctccaacggc
     6181 accatctacg atccggccgg catcgacgcc gagaagctcg cctgggtcat ggaactgaaa
     6241 aacgttcgac gcgggcgcat tcgcgaatac gccgagaaat tcaagggggt cgaatacaag
     6301 gacggcgtcc ggccgtgggg cgtcaagtgc gattgtgcct tcccgtcggc cacgcagaat
     6361 gaaatcagcg gcgaagatgc caagacgctg gtgaagaacg gttgtaagct ggtctgcgaa
     6421 ggagccaaca tgccgaccga accggaaggc atcgaggtct tcctgaccaa caagatcctg
     6481 tatggccccg gcaaggccgc caacgccggc ggcgtggcca cttccggcct ggaaatgtcg
     6541 cagaacgccc agcacaccaa ctggacgcgg gaggaagtcg atcgtcgtct ccacgacatc
     6601 atgatcgcca tacacaaagc cgtctccaag accgccgccg agtacggcga gcccaccaac
     6661 tacgtcatgg gggccaacat cgccggcttc gtcaaggttg ccgatgcgat gatcgaccag
     6721 ggtgtggttt gatacacacg ctcagtcgtt gatccgtcgc gcgaacgcat ggcgcgatac
     6781 cggcttgtcg cacacggcgg caccgattcg gcgttgaagc gacgcggaag cttgcccgac
     6841 gccggcaaag gtcgccgaag gcaagcggcg ccaagggccg aagaacgcgc gtgaggcaac
     6901 gcagcctgtc ggtcgcggcg catcgaatct tgttcaatca tcgctgcccc gggaggctcc
     6961 gaaccgcatt cattgcctct tacgggcgcc cgcttgaccg aatacttgtt ttgttgatta
     7021 ctgtttgacg cccagtccca gcagaaacag gggccggagg atgctccacc agccgacgac
     7081 gggtttcatc ttggtgtagc ccagtcgcct gggcgggtag atcttcgtgc acggcacttc
     7141 cgcgtgccgg tacccgagct tgatggtctt gaatagaagg tagacctcca atccgtaacc
     7201 gtcgagccaa gactgattga ggttgatccg ccggtcgtca agcagcgaga ggcgaaaagc
     7261 tctgaatccg ttcgtgctct cggtcagctt cttgcccaca agcaggctga gcaaccaggg
     7321 gtgcagccgt gtcgccagct tgcggtagcc gggcatgtcg cccccgtagg ctccgccgac
     7381 aaggaaacgc gagccgataa cgaagtctat gttctgatcg cagatcggat caagcagccg
     7441 agggatctcc gcggggttgt ccttgttgtt gccggccatg atcacggcaa cgtcgtgtcc
     7501 ggtctgccgg ccgtactcga tgcccatacg cagggctcct cccacgccca tcctgtggcc
     7561 ggtggatagg accgttgccc cgccctctcg tgcgacctcg acggtgcggt cggtcgaggc
     7621 gtcatcgatg accaggatgg tgtcgaccag cttgcggtcg gtacgggcga cgacttggcc
     7681 gatcttggct tcttcgttgt gggccggggc catcgcaatg atctttttac ccttgtacat
     7741 cctgtcaatc cgcctcctcc aagccatcgt cgcgcagaac gaatcgacgg gcgcaccgtg
     7801 ggcaggacaa tgattcgtcc agccgcattc cgcaccggca catccagcct gccggacgcg
     7861 cagggttgcc cacgacgaga cgatggtccg gaacgttgcg ggtaaccacg gtgccggcac
     7921 cgacggcggc aaaggaaccg atggtgacgc cgcagagaat gaccgccccg gctccgatcg
     7981 aggccccgcg gcacacgcgg gtcggcactc gccagttgtc gggatgggcg taccgggcgc
     8041 gggcgccggg catgcgcgga cttcgggggt agagatcgtt ggtgaacacc acgcctggcc
     8101 cgatgaatgc gtcgtcctcg atcgtcacgc cgtcccagat gagtgcgctg tttttgatgg
     8161 tcacgcgatc gccgaccacg gcgccgccct cgatgaaggc atgatcgccg atgttgcagt
     8221 ctcgtccgac gacggcgccc ttcaggacat gggcgaaggc ccagatgcgg gtgccctccc
     8281 cgacgtccgg gctctcacaa agggccgacg ggtgaacgaa tgggcctgct gaaaccgatg
     8341 acgctggcat gttggctctc gtctggcgaa caaagcgatg caaacggtac gacgccctcg
     8401 gggccgcctg cttgtccctg ctcgaccttg cctgagacgc aggccggcaa agccgccggc
     8461 ggtgcccgat caggccacct cgcacaatgc ttcggggact tcggcctcga cgcgacacat
     8521 ggtcccgccc gcggccatcg agcgggtcgc cgcttccagc acggcgacca cctcgagacc
     8581 gtagcgggca tcagacaacg atcgcttgcc gccgagcacc cagtcgagga aggcctgtgc
     8641 ctggttgacc aatggttcgg actgccggat tgccggaacg tgcatgtcgc cgctgcgcag
     8701 caagtagcga aactcgccga aggagtcgta gtgtggctcc tggtcgatgc ccttgtcgta
     8761 gagcctcagg gtgttatccg gatcgatatc gtcccaatag gccatctgcc tgtcgccgac
     8821 cacggtcatg gttcgcacct tccgcggatt cagccagctc acgtgaatgt ggccgagcgt
     8881 gccgtcggga tacttcagtg tcgcgaagca gacgtcctca atgttcttct gcgagatgca
     8941 ccgaccgacc gcggacacct ctaccggcga gcaccctatc aggtagttaa aaatgctgat
     9001 atcgtgtgtc gccagatcgt acaaggcgtt cacgtcgccg cgaatcggac cgagattggt
     9061 tcggtttgcc tccatatagt gaatacgtcc cagctcgccc gattcgacca gcttgcgaag
     9121 ctcggtgatt ccgttgttaa acaggaacac gtgaccgacc atgaatacaa gaccggtgtc
     9181 ctcggccagc cggactattc cggccgcctc gcgggttgtg gcgcagatgg gtttctctgc
     9241 cagcacgtgc ttgccggcct gcagggcttc tcgggcgatc tgcgcgtgcg tgaaggtcgg
     9301 cgtggcgatg accacggcat cgatcgactc gtcggccagc agctcgtggt agtcggcgac
     9361 ggcgcgaatc tgcgggaacc gcttgagcat gttttcccgt ctttgctggc tgatgtcggc
     9421 acagcccacc acggtggcgc ggtcgctttc agagaagacc cgaatgtgat tgggtcccca
     9481 tctgccgcac ccgatcaccg ccagccgcac ttgcgctcca tccgctgcca tggtcccgat
     9541 actccctgaa gatgacgatc agtcacaact tcatgagata tcggaagtga ccagggtccg
     9601 atcaaggaac ttatcggagt cgcaacgggt gttgagatgg ttcaacgccc gataatgccc
     9661 ggtcatccgg gtgctgatgc cgtctgcgtt gccggagtca caaggaagag aacatggtgg
     9721 ccggtgttgc ccaccggctg gaaaccaggt gcgaaatcga tcggcagacc ccacggacgt
     9781 tcgtccgacg tcggccgacg ggcaatgacc gcacgggccc ctgtgctgcg aaacgcttca
     9841 atcacacgac cacgagtcgc gtcatcagaa agccagaagc tctcttcggg acgcagctcg
     9901 gagatgatct gaacgcctgc cagtctggcc cagtaagacc agctcttgag tgcttggcct
     9961 atgtgggcca ctttgtcgcc ggggcggaga cccatctccc caagctgccg ggcgatctgc
    10021 cattgggggt ggggcgcggt gtccgtgcgg agcctgagat cgctgacagt tcgactgatg
    10081 gccgggcagg cctccgtgcc aatggtcagc agcaaagcca gcagcgatac gccgcaaacg
    10141 gctcggatca gactgccgga caggttccct gccggcaagc gcacggcggg caacagtgct
    10201 ccccatccca agaggacaaa gggcgccacg taccgaggct cgacgtgaac gagggcgtag
    10261 atccccagcc cccccagcgc gggaagcagc aacggccaat atgaggagat ttcgcgcaat
    10321 gccgacccaa gacgactacc gaaggcgaaa agcaccagaa ccggaaaaag ccatgcgccc
    10381 tgttgtctgc atagcaggtc aaagtaagcg tccttgttcc gccagagcgc cctcgcctgg
    10441 cgcgacaggt tgaaggtcgg tttcacgccg tcataccaat aggacgggtc gtaccagaga
    10501 ggataagtcc ccggcaccgg gccggcgaac tcaaagacgg ccggcttgtc caggatttga
    10561 cgggaggggt gaacaggcgt gccggttccg ggcggcagac ctcgccaatg gtcccaggga
    10621 tagatgccgc tgacgtgagc tgcataattg atccggccgg agtcgccgaa cgtcaaacgc
    10681 cccttctgga cggacaggaa cgcaacataa ggcccggcag gcaatgcgaa acagatcagc
    10741 acgacgacca ccctggccgg caatctgcga aactgacccg ctgcaagcag ggccacgata
    10801 acaaacacca ctgccatcgg cagcatgatc gccttggtca gatagccgat gctcaggacc
    10861 aggccgagcg caacgaacca cccgatgccc gcttgcccgc cttgaagacg aacgacgata
    10921 cctgcggcca agtatacaca gactgcaacg cacatgtccg ggttgacgat acgcagggtg
    10981 atcaagtcaa gcgatgtcca gatgaacagg gcgtagccca gaacgaacca ggcccatgaa
    11041 ggcaattgct cacccgcgtc tgcggcggcg tctctggcac gtgatcggcc tcgatccaaa
    11101 atcgcaaccc ataggaagtg aaaactggcc aacgcacaga gatagacggc gaagttcaca
    11161 gcatgagcaa cggttgcttc ccaatagggg ctcgctccgc tgaacatcag gccgacggcc
    11221 agcagacatg agtaaaccgg gctccaatac ccgctgacgg catccgacca tcgcccggcg
    11281 cgcaccgcct cagccaggtc aaggtaagat atgccgtccg gattcatggt gtggcggtcg
    11341 ccccaggcgt ggatagcgcc caggccaacg gcaaccagcc agcagcacaa gagcagaccg
    11401 cgtcggaacc agggactgtc gggcccggtg gttacggcgg gtggcaattg acccggttga
    11461 tcctcgggag tagtcatgtc gtcccataac atcggtcgac cgtcagcctg agtattgctg
    11521 tccgccggaa atcttgcccc gccagcgacg tcccataaca atgaacggcc atcgggctgc
    11581 tgctgccgac caacgtccag gtcgagcggc tgatcggcct tcggaggatg cgatgtcctt
    11641 gccaatgcca acaagcgagg ttctggccgc gattcgaagc ggctgcgtga ttcctgctca
    11701 tccgctcgcc ttgaacgcga agcgaacgct ggacgaacgg cggcagagag ctctgactcg
    11761 ctactatctg gcggccggtg ccggtggtgt cgcggtgggc gttcatacga cccaattcgc
    11821 gatccggcat ccttcaatcg gactgcttcg gccggtgttg gagttggcga gcgaggaagt
    11881 gaaccgtttc acacgaacag gcggcaaatc cgcgatcatc aagatcgcgg gcatctgcgg
    11941 cgagacccgc caggccgtcg aagaggcgca actcgcccgc gaactgggtt acgacatcgg
    12001 cctgttgagc ctctcggcca tgaaggatcg ttcgcttgag gagatggtcc ggcacgcccg
    12061 ggccgtagca gaggtcttgc ccatcatggg cttttatctt cagccggccg tcggcggacg
    12121 cgtgctgcct ttcgccttct ggcggcgctt ggccgagatt gacaacttgg tcgcgatcaa
    12181 gattgccccc ttcaatcgct accagacgct cgatgttgtt cgggccgtgg ccgagagcgg
    12241 tcgagcggac caagtggccc tttacacggg caacgacgac aacatcattg ctgatctctt
    12301 gaccgcctgc cgaatgaatc acggcgggcg gcaggtgacg ctgcggattg tgggcggctt
    12361 gctcgggcac tgggcggtgt ggacgcggcg ggccgtcgag cagctcgacg ccattcgacg
    12421 cctgatcgcg ggtcacccgg agcccgccgg cagcccttat gccggaatga ccatcgcaga
    12481 ctggttgcga cagggcattg aggtgaccga cgctaacgcc gcgctcttcg atgcggccaa
    12541 cagctttgcc ggttgtattg ccggcctcca cgaggtcctt cgccgtcagg gtttgcttga
    12601 agggcgatgg tgcatcgatc cggccgagga tctgtctccg ggccaggctg aggagatcga
    12661 tcgcgtttac gccgcctatc ctcatctcaa cgatgatgcc ttcgtcgccg ctcaccgcga
    12721 taaatggctg agcggatagc agcaatcgcg atgtcgcgtg accgcgggaa tgtggggcaa
    12781 ctgctcggac gaagcatcgc tgcaagcctg gttcatgttg cctctggccg cacctctggc
    12841 gtccgggagc gttttgccct ataatatggg tgtgctcgct tctttcagtt gtgatgcaat
    12901 aaatgagcgg gttacggaaa gggcggcgat cgtgattcag atcagcagtg gtgttggcat
    12961 cagcgaatgt gaactgagat tctcctttga ccggagtccc ggcccaggcg ggcagaacgt
    13021 caacaaggtc aacacccggg tgacgcttga gttcgatgtc gaccggagca tgtcattgac
    13081 cgttgcgcag cgaacccaga tccgtcgcgc cttggcgagc cggatcgggc aggatggcat
    13141 tctccgcgtg gtttccagca aagagcggac acaactcggc aaccgccggg ccgccgtcaa
    13201 ccgcttcatc gagctgctcg gtgctgcttt ccacgccccc aagccccggc gcaagacctc
    13261 gcctccggcc tcggccaacc gccagcgcct ggaggtaaaa gccaaacgca gccaactcaa
    13321 acgctcgcgc tcggccgaga tagcggtcgg agaagaatga ccgcgcgtgc ccgtcggcgc
    13381 ttgctcgggt gcaactccgg attatccaag ggatgaacga gcacgtcttc tcagagcggt
    13441 cctgccgggt gtcgaaacgc ggtacggcag agaggtgacc tcactcgtcc gccacacaag
    13501 tgtcgtaaaa ctcaacgaag ttgttgcggc cgttgccgct cagcatcttg atcgccgacc
    13561 ttggatgctg gttgagttgc ccggtgatga tgtatggcac catggggccc cactccaccg
    13621 ccttgcgcat cggctcgaca tggtcctgca gcagcttgag tatcggtctg agccggaatt
    13681 tcgggttccg aaggaaaccg atcagcagtt ccatcgggca gttgcccgcc cctcgaccga
    13741 gaccgcccat cgtcgcgtcg atccggttcg ccccatggat gatcgcctct atggtgttgg
    13801 cgaacgccaa ctgctggttg ttgtgggcgt gaatgccgac ttccttgccg gttccattca
    13861 ggtaagactt atacttctta accaaaatct caatttgctc ggcataaagg ctgccaaaac
    13921 tgtcaacaac cacgattgtg gaagccggtg acgcggccag cacctcgagt gcctggtcca
    13981 gatcgggttc ctgcaccgtc gacacggcca tcaggttaca ggtaacctca tagcccttgt
    14041 cgtatgcgtc attgatcatg gccacggccg tgggaatctg gtggacgtac gtagcgacgc
    14101 gcaccacgtc gatcacgctt tcgtcctttg ggaggatgtc ctccttgtag tcgcacttgt
    14161 ccgcatcggc catgacggcc agtttcacgt cggtctcgtt atcgccgact actcggcgca
    14221 aatcgtcctc gtcacagaac ttccaatcgc cgaacttgtc acgggcaaag agcttcttcg
    14281 acgccttgta gccgatttcc atgtagtcga tacccgcttc aacgcaggtc tggtaaacgg
    14341 ctttgacgaa cttttcatcc cactgatggt cgttgatgag gcccccgtca cgaatggtgc
    14401 agtcaaggac cttcagttcc ggccggtaag tgatccatgg tgctttcaca gctaaggctc
    14461 cttaagaaac tcacagctct ttatcttgtg agtttccctg gcgacagtca aaccgcctgg
    14521 catgtcttcg ccataggtcg gcagaccagc cgcgattctt gccattccgg ccgcgctatt
    14581 gtcattcaga tgcccggccg ataaactcaa gggtccagat gggggcgtag ctcagttggg
    14641 agagcgtcgc gttcgcaatg cgaaggtcgt gggtccgaat cccatcgcct ccagtaaacg
    14701 taagtctttt tgcccaaggg aataacgaac aatcgccggc gctgtcgcac ggaccgcggc
    14761 tcggctccgg cagtctcgag atcttttcgg tcggcgagcg tcgccaagtc ggccggtgat
    14821 acgggcggtt ttcgcgcgcc acctattgcg ccccccaata cctgttcttt gtgtgcaggg
    14881 ttgccgtcga tcagcgacgg cgtgtgtccc gacgtccgcc gccggtcttg tcgcctctcc
    14941 gggccgtttg atggcgtctg aggagaaagg cgcatgatgc ggcatagaaa tctataaaga
    15001 cttctttaga acataatttc ttccggttcc gccgcccgac gttcttgacg gcaggaaatc
    15061 agaaggtgag ggtcgttagc ttctcgccat tgggcgatac tggactcgaa ccagtgacct
    15121 cgtccttgta agggacgcgc tctagccaat tgagctaatc gcccgcagat ggacattgtt
    15181 ctgtttcggg ccgggccagt caataggggg ctgtcaaccc gttaccggtc gtttgcgggc
    15241 gacacagaac aggttggccg attcgtatcg caggtgatcg ccccgctgga gcttctgaaa
    15301 catgcggttc acgggaccga ccggacgcgt gagatgatgg aatggtgcca gaagtcgcat
    15361 gaaaaaaccg ctgccgtcaa acaggctcac ctcgaagccg gcaccggaca gcaacccggc
    15421 caggtcctcg cgagaaaagt gctcatgcca tcgtttttcg gccgagatgt cgcctatcag
    15481 tccgtccggg ttggccgcgt aacgatgttc ataatcttcc tgagaatgtc gcagacggta
    15541 gaaccatcgg tgcagccgcg gaaaacgaaa cttgagattg cccaggtcca aacaggaaaa
    15601 gacatggagg cccggcaccg tgacgatcag tactccgtca tccttgagca cgcgatggag
    15661 ctccgcaagc aggcctttct gattcaccac gtgctcaagc acgtcgagaa ggctgatgga
    15721 atcgatctcc cggtctgcga acggcaatgg gagcggttcc ctgcggtgga tgagttccaa
    15781 tccgggccgc tcgcggcgtc cggcctcgat cgcttcccgc gaggcgtcaa tcccgatcag
    15841 ccgctgaaag cccttgtccc tgagcgtttc cagaaaacgc ccgctcccgc agccgaaatc
    15901 caggtggcac cgcctgccgg atggcacgtg ctcccatgca aagccgtgac ggccgggacc
    15961 gaacggattg tccgggcgaa gcacggttct catcggcggt cctgtcggtt cgatggcggc
    16021 acgcgatgcc acgcgcccgc aacccaatac gtcagggcgg tccggcctcc ggatggcagg
    16081 ccggtttcca gttggcccgt cctgtttaga atgcttactt tttcttccga gcggcgcggg
    16141 ctctgctggc gcgggccgct tgacgccgga cgatgccgtt gactttcatc ggcaggccga
    16201 acaggcgaat gaagccgatg gcgtcggtcg agtcatactc ggcgcccatg gtgaagctgg
    16261 ccagcgccgg gatgaacagc gaacgggggc tcttggttcc aaccgcacgg cagttgccct
    16321 tgtagagctt gatccgggcg gtgccggtca gcggcttgtt gacctgcgtg aagaaagcgt
    16381 cgagggcgtg tctcagcgga ctgtaccact gaccgtagta gaccagttca gcataccggt
    16441 gcgacacgat ctccttgtaa tgcagcgtct cgcggtccag gcagatgcct tccaaagccc
    16501 tgcgggcctc atacagaatc gttccgccgg gagtctcata cacgccccgc gatttcatgc
    16561 cgaccagccg gttttccacc aggtcgacct ggccgacgcc gtgcttgccg cccagctcgt
    16621 tgagtctggt gaggatcgag atcccgtcca tccgcttacc gttcaaaccc accggcgtgc
    16681 cctgctcgaa attgacttcg acataagccg gcgtgttcgg ggccttttcg atcggcacgc
    16741 tcaggaccag catgcgatcc tgcggctcgg cccaagtgcc ctccagctcg gctccctcgt
    16801 gcgagatgtg ccagaaattg cgatcgcgag agtagatctt cttcttggat tgctcgatgg
    16861 gaatgccgcg ggcctcggca taggcgacgg ccgcctcacg gctgcgcagc tcgaaacgcg
    16921 ggtccttcca gggggcgatc accttgagcg acgggtcaag cgccatgtag gtcagctcga
    16981 agcgcacctg gtcgttcccc ttgccggttg ccccgtgagc cacggcgtcg gcaccatact
    17041 cgtgggccac cttgacctgc tccttggcga tcagcggccg tgcgatggag gtgcccagca
    17101 gatacttgcc ctcgtagact gctccactcc ttagcatcgg ccaaaggaag tccttgacga
    17161 actcctcgcg cagatcgcgc tcgacatact cgctggctcc gctgtcgagg gccttcttgc
    17221 ggaggttctt aagctcgtcg ccctggccca gctcggccgc aaaggcgatg acctcgcagt
    17281 tgtacgtttc cttgatccac ggcagaatca cgctggtgtc cagcccgccg gaataggcaa
    17341 gcacgacttt cttgacttgg agtgccattt tctgttcctc aattcgcagg ttctgcgccc
    17401 acagacagtc atctcccggc tttcatggat caacaagatg gtccacaaca acggaccgga
    17461 ccggccatgc caggaaagaa caagattatc cgcgatctgc ggggcggtgt ccagatgaga
    17521 gcgggtgatg aggttatctg agcctcgcag gtggtggggg ctcatcgagc cactccagcc
    17581 gtaggtggac ggaacgctcc ggggcggtat ccacgaaacg cacgtgaaca acgagcggat
    17641 ccgagccgat agtcaactcg attcgtccct ctttcgggtg ctggtagatg accgggtcga
    17701 gtgcggccat gactttgagc ctgttcacca tcgattcaaa ggttggtatc ggcgcgttcg
    17761 gatcgagttt cgatcgtggt aagggcatcg atctgctcaa agtgagttcg tcgatactgc
    17821 ccttcgccat ctccaacaac aggaggttga cgtacttctt ggtggggctt gactctgcga
    17881 gctccttcag ttccgcaagg tcaatcgacg ctccggaggc ctgcagcaac ttgatgcggc
    17941 gccggaaccg tctccaccag ccgaaaggcc acatggccca aacctcccgc tattgaattg
    18001 ttctccgcaa gatgcctccg gtcaagcttg gatcggcttt ctgcccccga tcacgcacca
    18061 caggtggtac ttgacgaaga tgtcaactcc cgcgaaaccg gtgtccttca gccaccgcat
    18121 ctgatcgatg aaaggctcgt gcaggtcatc acctttggtt ttcggctggt cgatgcgttc
    18181 gatgttgcgc cgcttccagc cgtcgaaatg cccgcaccat cgccgagcat tctcgaccaa
    18241 tgcaggtggg atgtgatcgg cccggccttg gacgtggcgc acccagaaat cgaggctgga
    18301 ccgataggcg ttttcgtcga gtgtcttcat ttcgtcgccg ttgataaacc agcccccctc
    18361 ggccaacagt ccaaagcagc gagcataaag ggctttcttt tcgtgccgct ccagatgatg
    18421 gatggcggac atggagaata cgcaattcac gggatggtcg atctgccgcg tccagtcacc
    18481 ttccataggc gagagaatgt agttgatctg ctcgccgaat cttgccagac gccgcctcgc
    18541 gacggcgagg aaactctcgg atgaatccac ccagtagcag gtggcctgcg ggcagaccgc
    18601 cagcaccctc tccagaaatc ggccgccgcc ggcacccagg tcgacaattc gcggccttgg
    18661 cattgcggcc aagtcggcta atcgaatcat ctcctgctgt atgaaatcgt acatcggcac
    18721 caggaagggg gctacctggt catagtcctc ggccatatgg gcaaaacgct gcttgtcatc
    18781 ggtctccatc atcttgtctc cggtatacga gtgattgcgg cgtcgggatt cggctcagcg
    18841 cccgagacgc cgcagcagaa cgtcgcgcag aatgtcgacg ggcgccggcc ggccgatcca
    18901 gcgctcgaac tgggcggcag cttggttgac gaacatcgat accccgtcga cggttcgaca
    18961 acctcgcagg cgggcttctc tcaacaggcg ggtttcgatc ggattataga tggtatcgaa
    19021 gacgaccggc tttccgagta aggcgtcggc gggcaggggg gtgtcatcca cattcggcca
    19081 catgccgata ctcgtcccgt tgatgatgat atcggcctca tgatcggccg cttggttcaa
    19141 cggccgtgcc tcggccccga actcggcggc cagcgtcctg gcccgctcga aggtgcgatt
    19201 gtaaatcgtt acccggcagc cgcagtcgcg caaaccggcc acgatggccc ggctgacacc
    19261 accggccccg agaacggcaa ccgagagtcg acccaactcc tcgcgcgtac agcagatgcc
    19321 tgcacacagg gcgtccatcg cgccacgata atcggtattg caggcaccg
//
LOCUS       DAOTPW010000098        19347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2887_length_19347_cov_5.263322, whole genome shotgun sequence.
ACCESSION   DAOTPW010000098 DAOTPW010000000
VERSION     DAOTPW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19347
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_19347_cov_5.263322"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3109
                     /locus_tag="P5316_10540"
     CDS             <1..3109
                     /locus_tag="P5316_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85435.1"
                     /translation="VALDAGQTRRFRIVRRDPREGFSVAVVPDFGPKYHERRIDYDFA
                     RVHSWSGTQSFDPPVPVEEVGPPIRSVEANKPSIRSSQVYFQLKGLPPLRSINAVGLP
                     RTSLIDWREAKYECADGSEYTCRYGTIPDEPVVVVHEDFRKLDAGEMVVELSDPAGLT
                     HGHGRGRLAPPGEGKLTSGYAIDFSKEWTGRIQPFYAWWKDYGLWWAAYRPEGGYVGI
                     LPLKPSEWANPAPNVIRISTGPGGRARAVLPFHQGARTWAIVLSPAAEALRNKPTGAS
                     LMMLQAVRLGQNPLDRVKDMVLNWPGMDEIEYPHLLCRPADIPAIRGKARTHPLFKRV
                     LEDHPDAPEDPVGLYLATGDERVARRAIDELMTQLRKWVDEALEGGNYGESLCAIPFT
                     RPLRTLALSFDVLASSPSMTAAQRNWCLRTFAFFAYCLYDENRWPGRNQGFHRGNVNF
                     HSDDYTCRAAVAALLKGHPKQKEWMGYVEREMRLEFDQCVSPGGAWCEAPNYQGFTMH
                     YLIIAMRLMQLNGFADFADEPRFRETMDYFFRIQTPFDGRAGCHMLPTVGDTTSSYHS
                     QSLQNVFAWTAALLREKDPTFAGRMMHAWRRGGSVLFGKHGLGFGQGWLQPLLLIDPD
                     IPEVAPPTPLTSERLPGYGVLFRNKYGTDRESYFLFKMGPIDQHFDCDEGSFHWYALG
                     RPISLDFGSMYRPSIEQPWLHSTLDFDRRRVWGQGEISQFVSLPDVDFCRGEILVRSL
                     QRVPDLPEGPLPPGVNGEIKWNRYQIPWRREVLFVKGPDYVVIRDDVGTDASRFSTGW
                     TVQVLASDVEVTGPMARCKGQYGIDMAVLVAEPPVAQLTTSRWGYESEPAPAWVAPDP
                     MPAVAENQIALHVTAPADADYLAALVPHKQEEPPPKPASPGPGVLTIEHSAGRDLVFF
                     YPDAQRQTIDAVTFDGRVAVVSKTEQETMIHILDGRFIGTAGITVRCEGPLTLRLADD
                     RITGVTDGPARTCTVNCPAIQGRTPRATIDGQDAPVDARNTGELGLRLPAGRHEFSLS
                     TVTNR"
     gene            complement(3221..6610)
                     /locus_tag="P5316_10545"
     CDS             complement(3221..6610)
                     /locus_tag="P5316_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HRR85436.1"
                     /translation="MFRGLIAGLVLVSLITPAVAADETQVVWQIGRADNDYRDLAHLT
                     NLADFGKTFPDGVKFTVGKSDPAKDFPFIHPGPADAWAGKREHPFTINFELAAVPDDG
                     CELRVDLVDAHADAPPRLIVEINGQRGEVALTPGTGDPTLIRPEKGKNRTLRFFCNTS
                     VLKQGANKITITATRGSWFLYDAITLARLPKEVASALELAVTPSIFFVERDGRLLQEF
                     NCYVAGVLSSEPVVIEVRTGDGLLSSGRLAQASLGTLSGQVLIPPAESTRSLVIRAIC
                     GKQTAVVTVDQKPQRKWHIFCAPSTHTDIGYTDLQENVIALHNRNTDLALQLIQEFPL
                     YHWNLESSWAAQMWFRDRPQWRHKELYEASKNRRVGIEAGYLNMLTGLCSEEELVRNL
                     YYSARLHRQHGVPFESFTLTDAPSHVWSIPTILAGAGIRYVSVGINGIRAPLLKQNLH
                     HKSPFWWEARDGSKVLTWFTPGYSQAGQIGLNEGPERMRAAIEKDLYWWDHREDYPFD
                     AILLHGAYSDNVAIGRAIAESLTEYSKRYAYPKVILCANNHFFEHIEKNFADKIPTVR
                     GCGGSWWEDGAASSAFETAVNRVAHQDAVAAEAIWAAGGKDKATTGAQSAFNRVWDNI
                     LLYDEHTWGAWNSISAPTSDFVTRQFAVKAAYATDAARQIRQLMSRGLNRLAARVNVP
                     AGSLLVFNPSGSVRTGVVHAEIPRHMLVMDEDGPLPQQVVKEDVLENVTVALLARDVP
                     PVGYRTYRVAKDTGKLANPPARFKDNVLENDFYRVTFDPKTAAVASIFDKMLKKELVD
                     QSSKYKLGQVIYGVGGDFTGEKEWWGPDPAKIVFNELTGKSVEAGARGPLFSSAKCKA
                     EMKGFRSVELETILYEQERRIQFVVRLNKEMTFDREALYVAFPFAGANPRFRYEIGGG
                     HVKPNDEHLPGGCRDWFAVQRWVTVHTDDAAVIWSPVDTPLITLCDLTPGKWLDTLPI
                     TNGTIFAYCLNNYWFTNYKAGQDGNFTFRFDLTSGKSFDPQSACVFGEAAVQPMRSIR
                     LHPGRGKKGLPASASFCQVGPANVMLTAFKDADDGRGVIIRLRETAGKDTTAVVKTVL
                     PDAKKAVLCDLVERDQGPAEFSNGELKVPVKADSMAAVRIE"
     gene            complement(7661..9604)
                     /locus_tag="P5316_10550"
     CDS             complement(7661..9604)
                     /locus_tag="P5316_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85437.1"
                     /translation="MLLCQLLLICRLAVLSPADYFKIQVVDAQTGRGVPLVELTTTHN
                     VRYYTDSNGLVAFYEPGLMDQRVFFTVKSHGYEYPKDGFGMAGTALDIKPGGSTVLKI
                     NRINVAQRLYRLTGGGIYRDTVLLGLKPPIREPLLHGRVLGCDSIQGAVYRGRLFWMW
                     GDTSWPAYPLGNFHMTGATSALPSAGGLDPDVGIDYDYFLRDNGFVKEMAPHPGPGPV
                     WLDGLVTLPDESGRERLYALYARVNTAMEAQERGLMLFDDQKEIFEKVVEFDLKAPVR
                     PAGHPFRCEVDGRRFVYYDPGYPFVRIAADARLLRDSANYEAYTPLVHGSSVESPTFD
                     RRPDGALRYAWKRNTPGLTPADFARMIKDGLLKQEEALIQTTDVETGKRFQLHGSSVC
                     WNEYRRRWVMIASEIMGTSMLGELWYLEADTPLGPWVYGRKIITHDKYSFYNPKQHPL
                     FAKDNGRFIYFEGTYTAFVSGTPMLTPWYDYNQIMYKLDLNDDRLALPAPVYELSFEP
                     GKQMLGRWDSVTPGRDFPKIAFFAPDRQFAGSVPVYQHKSADGAMTLIVGDTASGAGE
                     TADKPLFYALPAGIQSPPMALVGLHLSKAGDRSQAFYSVSATAPATGAAQADKPLCLV
                     WPSPYTAAPPLPPKEDIKPGPPQ"
     gene            9758..10573
                     /locus_tag="P5316_10555"
     CDS             9758..10573
                     /locus_tag="P5316_10555"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HRR85438.1"
                     /translation="MNIFISADMEGICGVFGLHQWESGGSGYERACEWMAAEVNAAIE
                     GAVAAGARRIVVKDAHCDGNNIDLEKLHPAAELISGWGPLNSMVEGISPEFHALILIG
                     YHARAATADATFAHTWSRNVLELRVNGTPVGEAEWAAAFAGHFGVPVAMITGDDKLAD
                     QVRETIPAGFEFVVTKTGWSFNAAQMRPIAMVRQEIRETTGRVLSQPRRLPVYRPSLP
                     ATITVRFRQWEGLHACEALPNVRRLSVDTFEYAAADFIEAHKYFSTLNRLAKP"
     gene            complement(11303..13336)
                     /gene="fusA"
                     /locus_tag="P5316_10560"
     CDS             complement(11303..13336)
                     /gene="fusA"
                     /locus_tag="P5316_10560"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRR85439.1"
                     /translation="MPNYSTTDIRNVALVGHQSSGKTSLGDAILHATGTTNRLGDVNA
                     KSSYLDFMDEEKNRGCSIDSALIHVTHKGRQINVVDTPGAPDFIGPAIAALAGVETAI
                     CVISATAGIEVNTRRMMERAKEHGLGRMIVINKIEANPDCAGVVAAVQELFGNECQPM
                     NLPAKKGRAVIDCFQQSDGQADILDVASAHTALIERVVEADEAMMEQYLETGEVDVTR
                     LGAVVAKAIVAGTFVPILFTDARGEVGVSELLDVIAEFAPSPLEGKQRVLKVDETETP
                     VRPDRNEKLVGQVFKVFSDPKSNIKYSVCRIHAGSLKSDSQIYVGDDRKSQRPGQLHK
                     LQGAEHPEIDEGIAGDIIAMAKIEAHIGDVVRFEAGGGTIAMPKFPHPMFSLAIEPKS
                     RGDADKVSGALARCCDEDPCFIADRDTVTHELVIRGVGDLHLRSILSRMAQYFKLEVN
                     TKPPKIPYRETITGSTKDVEYTHKKQTGGAGQFARVIIAMEPNERGAGYEFIDEIFGG
                     AIDLSFRPSVDKGIQAQMKEGVLAGYPVVDVKVRLTDGKTHPVDSKDIAFQIAGREAF
                     KKAFMQCKPVLLEPIVHVEVTVPNDKVGDIQGDLASRRGRPEGQEMLPGGLSVISARV
                     PLAEMSDYHSRLSSITGGQGSFTMELSHYENVPGNIQQQIIENARKAREEARA"
     gene            13876..15180
                     /gene="eno"
                     /locus_tag="P5316_10565"
     CDS             13876..15180
                     /gene="eno"
                     /locus_tag="P5316_10565"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRR85440.1"
                     /translation="MSSTAIVYVNGREILDSCGNPTIEATVVLENECVGQASVPSGRS
                     KAADEAVELRDGDMARYGGMGVLKAVENINERIAEAILGLDACDQESVDREMINLDGS
                     PNKGNLGANAILAVSMAVAKAAAEACKLPLFRYLGGVSAHVMPVPMMNMLDGGRRADN
                     NVDFQEFLIQPWGAPTFREALRWSAEIQQTLASILSERGYSVSVGDEGGFAPKLKNND
                     EAFELMAMAVEKAGYKLGEQIFFALDPAMSELFGEAKAKGKVGYCLFKSDPDRILGSD
                     EVIDCWLALCRKWPLRSLEDGLAEDDRDGWKKLTASLGGKIQLVADDLCSTLMNRLGR
                     GFREGIANSILVKTDQIGCLTETLNTINLAMRNGYTTVVSHGSGETEDTTIADIAVAT
                     NAGQIKAGGVRRSDRVAKYNQLLRIEEHLGDEAVFGGTFWNK"
     gene            15202..15831
                     /locus_tag="P5316_10570"
     CDS             15202..15831
                     /locus_tag="P5316_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85441.1"
                     /translation="MDADTCPTEQDNQTAIHPEQGAQGGGRADSPGGVVAQHCIMFWA
                     LTVLAMAVFAPCVLAPIYAETEQIMETEQKLERLVADLKDQANRNEERIKAIKADPQV
                     NQRIIRRELNYRFEGEQIIQWPAAELASTRLDLSMLEGAQEPLPPINPYPAWVLRLRQ
                     WLPAWPWHKLFVESPNRELLAIMAAGLLATAFFLYGPLPPIAGPQRDDR"
     gene            16020..17240
                     /locus_tag="P5316_10575"
     CDS             16020..17240
                     /locus_tag="P5316_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85442.1"
                     /translation="MAFDRGSFSFKRFFVSGRSPQRVDEALLGQLAAKAIGADSIQTA
                     DHSEIGWSTGEHILDTEFDFAKNAVADGLYFALRIDTNKPPTELVRSYQKINEQAMLR
                     ATGREFLSKAQRREAREQALARLDSEAKSGAFRRMKQIPVFWDLTRNELYFGSTSNSA
                     AESLMLLFRETFDRGVVAASAGEIAARWAAASGENRLFEDCRPAHFVTPPEGAGETPD
                     AHSDEGRTNDFLGTEWLTWLWYATQVESSTITTAKSGAVTVLFEKSMQMQCAFRMSGS
                     VAVSSDSPTRLPEALVALSGGKQPVRAAMQIEAQGSIFSFALRGDAMAFSGVQLPAPE
                     DANSVRAVFEDRMSRLRDLIDAGDGLYAAFLKRRLSSKWPQTLSAMRAWIASCRHAAS
                     SQDMQTTAMLGAVS"
     gene            17532..18092
                     /locus_tag="P5316_10580"
     CDS             17532..18092
                     /locus_tag="P5316_10580"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRR85443.1"
                     /translation="MSQVTGPAYKHEDYAGFIRRTAALLFDIILVILASILIGGICIS
                     ISSSPWTEEQEDAVLAFVFTIAFVLYMLGFRLSLKGTPGYRLFRIRYAYMLDGKPTAL
                     VLLFRSFVAVVLLLFFALDHLWILFDEHKQAWHDKLSGFYVVKSKARPCGKQRVTRRI
                     INFMMLTFIVWEAGPETHAGTAAGAT"
     gene            18168..18917
                     /locus_tag="P5316_10585"
     CDS             18168..18917
                     /locus_tag="P5316_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85444.1"
                     /translation="MQRFLAMCLLGCGAATFAAADTLAAARTEKKPDIRTQLEQEFEA
                     LHESLKKAMPEDPKTLRDPAVRAKVAPAAMPILQKTIECLERRARFDKSLAGDSAWYR
                     VIALALGNDSIKTNLTTAAESGDRGAKIQLAASRVIVAADTSQRKTAMNEFAALLGAE
                     GAAPPGAATALQAVVYAGALSKEEIKQISDVVEKLPDKTLKNALDLATMTPAERRAAL
                     RGKPLVLEGKLLTGKPFSTDSLKGKVILVHF"
     gene            18933..19238
                     /locus_tag="P5316_10590"
                     /pseudo
     CDS             18933..19238
                     /locus_tag="P5316_10590"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
BASE COUNT         3697 a         5922 c         6037 g         3691 t
ORIGIN      
        1 cgtggcactg gacgcaggcc aaacccggcg attccgcatc gtcaggcggg atccgcgaga
       61 agggttctcg gttgcggtcg tgccggactt cggccctaag taccatgaac ggcggatcga
      121 ctacgacttc gctcgagtcc actcctggtc aggcacccaa tcattcgatc cccccgtgcc
      181 tgtcgaagaa gtcggaccac cgatccggag tgtggaggca aacaagccgt cgatccgaag
      241 ctcgcaggtt tatttccagc tcaaagggct gccgccgttg cgaagcatca acgcggttgg
      301 gcttccgcgg acttcgttga tcgactggcg cgaagcgaag tacgagtgtg ccgacggcag
      361 cgagtatacc tgccgttacg ggaccatccc ggacgaaccc gtcgtcgtcg ttcatgagga
      421 ttttcgaaag ctggatgccg gcgagatggt tgtggagctg agtgacccgg ccggtctgac
      481 acacggtcac ggccgaggcc gtcttgcccc acccggcgaa ggcaaactga ccagcggcta
      541 cgcaatcgat ttctccaagg aatggaccgg acggatccag cctttctatg cctggtggaa
      601 ggactacggc ctctggtggg cggcatatcg gccggaaggc ggatatgtcg gaatcctgcc
      661 gctcaaaccc tccgaatggg ccaaccccgc tccgaacgtg atccggatca gcacaggccc
      721 cggcggccgt gcacgggcgg ttctgccgtt ccatcagggt gcgcgaacct gggcgatcgt
      781 gctctctccg gcggcggaag ccctgcgcaa caaacccact ggcgccagcc tgatgatgct
      841 ccaagcggtt cgcctcgggc agaacccgct cgacagggtc aaagacatgg tgctcaactg
      901 gccgggtatg gacgaaatcg agtatccgca tctgctgtgc aggccggcgg acatcccggc
      961 tatccgcggc aaggcccgga cccatccgct gttcaagcgg gtattggagg atcaccccga
     1021 tgctcctgaa gacccggtcg gactgtacct ggccacaggt gacgagcgcg ttgcccgacg
     1081 cgccatcgac gaactgatga cgcagcttcg caagtgggtg gacgaggcgc tcgaaggcgg
     1141 caactatggc gagagcctgt gtgccatccc gttcacgcgt ccattgagga cactggcctt
     1201 gtcctttgat gtgctggcat cgagcccgag catgacggca gcccagcgca actggtgcct
     1261 tcggaccttc gcgttcttcg catactgcct ctacgacgag aatcgctggc cgggccggaa
     1321 tcagggcttt catcgcggca acgtgaactt ccacagcgac gactacacct gccgggctgc
     1381 ggtagcggct ctgctcaagg gacatcccaa acagaaggaa tggatgggct acgtcgagcg
     1441 cgaaatgcgg ctggagtttg accaatgcgt ctcgcccggc ggtgcgtggt gcgaagctcc
     1501 caactaccag ggcttcacga tgcactacct gatcatcgcc atgcggctga tgcagttgaa
     1561 cggcttcgcc gatttcgccg acgagccgcg gtttcgggag acgatggact acttctttcg
     1621 catccagacg ccgtttgacg gccgcgcagg ctgccacatg ttgccgacgg tgggcgacac
     1681 gacctcctcc tatcacagcc aaagcctcca gaacgtcttt gcatggaccg ccgccctgct
     1741 gcgagagaaa gatccgacct tcgccggacg catgatgcac gcgtggagac gcggcgggtc
     1801 ggtccttttc ggcaagcacg gcctgggctt cggacagggg tggctccagc cgttgctcct
     1861 gatcgacccc gatattccgg aggttgctcc gcctaccccg ctgacgagcg aacgactgcc
     1921 gggctacggc gtcctgttcc gcaacaagta cggcacagac cgcgaatcat acttcctgtt
     1981 caagatgggg ccgatcgacc agcactttga ctgcgacgaa gggagttttc actggtatgc
     2041 gctgggtcgg ccgatttcgc tcgacttcgg ctccatgtac cggccgagca tcgaacagcc
     2101 ctggctgcac agcacgctcg acttcgatcg gcgccgagtc tggggccagg gcgaaatctc
     2161 gcaattcgtc tcgctgccgg atgtggactt ctgccggggc gagattctgg tcagaagcct
     2221 gcagcgcgtg ccggatctgc cggaaggacc cctgcccccc ggcgtgaacg gcgagatcaa
     2281 atggaaccgc tatcagattc cgtggcgtcg ggaggtccta ttcgtcaaag gacccgacta
     2341 cgtcgtgatc cgcgacgacg tgggaacaga cgcctcacgg ttctccacag gctggacggt
     2401 gcaggttttg gcgtcagacg tcgaggtgac cgggcccatg gcccgatgca agggccaata
     2461 cggcatcgac atggcggttc tggtggccga accgcccgtc gctcagttga ccaccagtcg
     2521 gtggggctac gagagcgagc cggcgccggc ttgggtcgca ccggatccaa tgcccgccgt
     2581 cgcagagaac cagattgccc ttcatgtgac ggcgccggcc gacgccgact atctggcagc
     2641 gctggttccg cacaagcagg aggagccgcc gcccaagccg gcgagccccg gaccgggagt
     2701 gttaactatc gagcactcag ccggccgcga cctggtattc ttttaccctg acgcgcagcg
     2761 gcaaacgatc gacgccgtga ccttcgacgg ccgcgttgct gtcgtgagca aaaccgagca
     2821 ggagacgatg attcacattc ttgatggccg cttcatcgga accgcgggaa tcacggtccg
     2881 ctgcgaggga ccgctgacgc tgcgccttgc ggacgaccgc atcaccggtg ttaccgacgg
     2941 cccggccagg acctgcacgg tcaactgccc cgcgattcag ggaaggacac cccgcgcgac
     3001 aatcgatggg caggatgctc ccgtcgatgc tcggaatacc ggagaacttg gcctccggct
     3061 cccggccggc cgccacgaat tcagcttatc cacagtcacg aaccgatagt cttcaacccg
     3121 cgactccgat cgccggcact cgtttaagaa ccaccggttc ccaccctttc ggccaatagc
     3181 tgacaagcaa aaactgacag cttcccttga ccgctcacag ctattcgatt ctcaccgccg
     3241 ccatcgaatc ggccttcacc ggcaccttga gttcgccgtt gctgaactcg gccggtccct
     3301 gatcgcgctc gacgaggtcg cagagcacgg cctttttggc gtcgggcaga acggtcttga
     3361 caactgcggt cgtgtctttc ccggcggttt ctcggaggcg aatgatcaca ccccggccgt
     3421 cgtcggcatc cttgaaggcg gtgagcatga cgttggccgg gccaacctgg cagaagctcg
     3481 cgctcgcagg caggcccttc tttcctcggc cggggtgaag ccggatcgac cgcatcggct
     3541 ggactgccgc ctcgccgaaa acgcacgcgg actgcggatc gaacgacttg ccgctggtca
     3601 ggtcaaatcg gaaggtaaag tttccgtcct ggccggcctt gtagttggtg aaccagtagt
     3661 tgttcagaca gtaggcgaag atcgtgccgt tggtgatcgg caacgtgtcc agccacttgc
     3721 ccggcgtcag gtcacacaac gtgatcagcg gcgtgtccac cggcgaccag attaccgcgg
     3781 cgtcgtccgt gtgcaccgtg acccacctct gcacggcaaa ccagtcgcgg cagccgcccg
     3841 gcaggtgctc gtcattgggt ttgacgtgcc cgccaccgat ttcgtagcgg aaccgggggt
     3901 tggcccccgc gaacgggaat gcgacgtaca gcgcctcgcg gtcgaaggtc atctccttgt
     3961 tgagccggac gacgaactgg atccggcgct cctgctcgta gagaatggtc tcaagttcga
     4021 cgctgcggaa acccttcatc tcagccttgc acttggcgct gctgaacagc gggccgcgag
     4081 cccccgcctc gacgctcttg ccggtcagtt cgttgaaaac gatcttggcg gggtccggac
     4141 cccaccattc cttttcgccg gtgaaatcac cccccacgcc atagatcacc tgtcccagct
     4201 tgtacttgct cgactgatcg accagttcct tcttgagcat cttgtcgaag atgctggcca
     4261 cggcggcagt cttgggatcg aaggtcaccc ggtagaagtc gttctcaagc acgttgtcct
     4321 tgaaccgggc cggcggattc gcaagcttgc cggtgtcctt ggccacgcgg tatgtccggt
     4381 acccgacggg cgggacgtcg cgggccagca gtgcgacggt gacgttctcc agcacgtcct
     4441 ccttgaccac ctgctgcggc agcgggccgt cttcatccat gaccagcatg tgccgcggaa
     4501 tctccgcatg gaccacgccg gtgcgaacgc tgcccgaggg gttgaacacc agcagtgagc
     4561 ccgccggcac attgacccga gccgccaagc ggttcaagcc gcgactcatg agttgacgga
     4621 tctgcctcgc cgcatccgtc gcataggccg ccttaaccgc gaactgacgc gtgacgaaat
     4681 ccgaggtcgg cgcgctgatg ctgttccatg ctccccaggt atgctcgtcg taaagcagaa
     4741 tgttgtccca aacgcggttg aaagcgcttt gagcaccggt cgtggctttg tccttgccgc
     4801 cggccgccca gatagcctcg gcggccaccg catcctgatg agcgacacgg ttgacggccg
     4861 tctcgaacgc gctggaagcc gctccgtcct cccaccaact gccgccacac ccgcggacgg
     4921 tcggaatctt gtccgcgaag ttcttctcga tgtgctcgaa gaagtggttg ttggcgcaga
     4981 gaatgacctt cggatacgca taccgcttgc tgtattcggt gagcgactcg gcaattgccc
     5041 gaccgatggc gacgttgtcc gagtaggccc catgcagcag gatcgcatcg aacgggtagt
     5101 cctcgcgatg gtcccaccag tacaggtcct tctcgatcgc cgcccgcatc cgctcgggcc
     5161 cctcgttcaa gccgatttgc cccgcctggg agtatcccgg cgtgaaccag gtcagcactt
     5221 tgctgccatc acgggcttcc caccagaagg gcgacttgtg gtgcaggttc tgcttgagca
     5281 gcggtgcgcg gatgccgttg atgcccacgc tgacgtaacg gatgcccgcc ccggccagga
     5341 tcgtcggaat gctccacacg tgggaaggtg cgtcagtgag cgtgaaactc tcgaagggca
     5401 cgccgtgctg gcgatgcagc cgggccgagt agtacaggtt gcgaaccagt tcctcctccg
     5461 aacacaggcc ggtcagcatg ttgagatagc cggcctcgat gcccacgcga cgattcttgc
     5521 tcgcctcgta gagctccttg tgacgccact gcggccggtc acgaaaccac atctgcgccg
     5581 cccacgagga ttcgaggttc cagtggtata gcgggaactc ctggatgagc tgcagggcaa
     5641 ggtcggtatt acggttgtgc agcgcgatga cgttctcctg cagatcggtg tagccgatgt
     5701 ccgtgtgcgt gctcggcgcg cagaagatgt gccacttgcg ctggggcttc tgatccacgg
     5761 tgaccacggc ggtctgcttc ccacagatgg cccggatcac cagcgaacgc gtgctctcgg
     5821 ccggcggtat gagcacctgc ccgctcagtg ttcccagcga cgcctgcgca agcctgccgc
     5881 ttgacagaag accgtcaccg gtgcgaacct cgatcaccac cggctcactg ctcaacacgc
     5941 cggcaacgta gcagttgaac tcctgcagca gccgaccgtc ccgctccacg aagaagatgc
     6001 tcggtgtaac ggcaagctcc agcgccgaag cgacctcttt cggcaagcgg gccagcgtga
     6061 tcgcatcata aagaaaccac gagccccttg tggcggtgat tgtgatcttg ttggccccct
     6121 gcttgagtac gctcgtgttg cagaaaaacc gcagcgtgcg attcttgccc ttctccggcc
     6181 ggatcagcgt cggatcgccg gtcccgggcg tcaaagccac ctcgcctcgc tggccgttga
     6241 tctccacgat cagccgcggc ggggcatcgg cgtgtgcgtc gacgagatcg actcgcagct
     6301 cacaaccgtc atccggcact gcggccagct cgaagttgat ggtgaacggg tgttcgcgct
     6361 tgccggccca ggcatccgcc ggacccggat ggatgaaggg gaaatccttc gccgggtcgc
     6421 tcttgccgac cgtgaatttc acgccgtcgg gaaacgtctt gccaaagtcg gcgagattag
     6481 tgaggtgggc caggtcgcgg taatcgttat ccgcccggcc gatctgccaa acgacctggg
     6541 tttcatctgc cgcgacagcc ggcgtgatca gtgatacgag taccagacct gcgatcagac
     6601 cgcgaaacat tagacacctc ccatctcata aggatacgga actgatttcg gcacgcgagc
     6661 ggcactacct ctggccggct attctacatg gctcgacgcc aggggggaat accacgagcg
     6721 aggcgtgatt cgtgcgtgtt gaatcgagac gtggcgcgct gctttaaaac ctcacggcca
     6781 gacgtgtgtg cagcttgtgc gcatgttgct gaatcgcagg tcagcttggg ttgcaacccg
     6841 caagaaggcg gtccgcctca ggctcaaccc cactcccctt tccttccgcg tcgtttccta
     6901 cgctgatcct tcgactcagg ctccttccct ccatggtcgt gacccgcttc gtcggtacga
     6961 cgagctcgtt cgaccccgtc cggaccggtc ccgcccctaa cgggccaccc gttgtccggc
     7021 tttcggccgc caccggaacg ggtctcccgt gttgcatctc ttctcctcgc aaatatgcca
     7081 tcgctactgc cccggcggac ccatggagct tttgccattc cgccttgttc tcgcttgggg
     7141 gcgagggctc cggtctccac agcggtagcc ttcctccttg gaccatgggg tccgcatccg
     7201 caagctttgt ttccgagctt gctcggcgtt ccctcaggtc acggcctgcc tgctcgccag
     7261 gctgccttca gcaacccttc tcgtcgaaag cttcgtctca tttttacttc tctgtttcga
     7321 ctgttaccgg gtggaacaac taatcccggg caggactctc gccgactgaa gaacaacgcc
     7381 ttttcagggc agacgatccg tgacagactt cgcggcaaac gcgagggttc accttaagaa
     7441 ccgcgaccgt aaaggagcga ccttcttatg tcgtggcaac cttgtggttt gcccctcgtt
     7501 tgcgctcgag gttccgagag agccacctgg actgccgcag acccgggcgg tcccggcgca
     7561 tcgcctcttg cccttgcctg aactgtctgc cgagcgttcg tgcttgccgg cccgttcaca
     7621 agtcgagaaa gagcccggtt ggcggttgac catgggccgc tcactgcggc gggccgggct
     7681 tgatgtcctc cttgggcggc aaaggcggtg cggctgtgta ggggcttggc cataccagac
     7741 agagcggttt atccgcctgg gccgcacctg tggcgggagc tgtcgcagac actgagtaga
     7801 aagcctgtga acggtcacct gccttggaaa gatgcagtcc gaccaaggcc atcggcggcg
     7861 actggatgcc tgccggcagg gcgtagaaca acggcttgtc agccgtttcg cccgctccag
     7921 aggccgtgtc accgacgatg agagtcattg cgccatctgc ggacttatgc tgatagaccg
     7981 gcacgctgcc cgcgaactgc cgatcgggcg cgaaaaacgc gatcttcggg aagtcccggc
     8041 cgggcgttac actgtcccac cgccccagca tctgcttgcc gggctcgaac gaaagctcgt
     8101 acacgggggc cggcaaagcc aggcgatcat cgttcaggtc gagcttatac atgatctgat
     8161 tgtagtcata ccacggtgtg agcatcggcg tgcctgagac aaaagccgtg tacgtgccct
     8221 caaaatagat gaaccgcccg ttatccttgg cgaacagcgg gtgctgcttg gggttgtaga
     8281 agctgtattt gtcgtgggtg atgattttgc ggccgtagac ccacggcccc agcggcgtgt
     8341 cggcctcgag ataccacagt tcgccgagca tcgatgtgcc cataatctcg gaggcgatca
     8401 tcacccatcg ccggcggtac tcgttccagc agaccgagga cccgtgcagt tggaagcgtt
     8461 tgcccgtctc gacgtcggtc gtctggatca gcgcctcttc ctgtttgagc agcccgtcct
     8521 tgatcatcct ggcaaagtcg gccggtgtga gcccgggcgt gttcctcttc catgcataac
     8581 gtaacgcacc gtcaggacgc cggtcgaacg tgggagattc cacgctgctg ccatggacga
     8641 gtggggtata tgcttcatag ttcgcagaat ctcttagcag ccgggcgtcg gcagcgatgc
     8701 gtacgaacgg gtagccgggg tcatagtaaa cgaaccgtct gccatcgacc tcgcacctga
     8761 acggatgtcc cgccggcctg acgggcgcct taaggtcgaa ttcgacgacc ttttcgaaga
     8821 tctccttctg gtcatcaaag agcatgaggc cgcgttcctg ggcctccatg gcggtattga
     8881 ctcgggcata caatgcgtag aggcgttcgc gcccggactc atcgggcagg gtaaccaagc
     8941 cgtccagcca gacgggtccg ggaccggggt gaggagccat ctcctttacg aagccgttgt
     9001 cgcgaaggaa gtagtcgtaa tcgatgccga cgtccggatc gagtccgccg gccgagggca
     9061 gggccgaggt ggccccggtc atgtgaaaat tgcccagcgg gtaggccggc cagctcgtgt
     9121 caccccacat ccagaagagc ctgccgcgat agaccgcccc ttgtatgctg tcacagccga
     9181 ggacccgccc atgcaacagc ggttctctga tcgggggttt cagcccgagc aggacggtgt
     9241 cgcggtagat gccgccgcca gtgaggcggt acagccgttg ggcgacgttg atccggttga
     9301 tcttgaggac ggtgctgccg ccgggtttga tgtccagggc cgtcccggcc atgccgaaac
     9361 cgtccttggg atactcatag ccgtggctct tgacggtgaa gaacactcgc tgatccatga
     9421 gtccgggctc atagaaagcg accaggccgt tgctgtccgt gtagtagcgg acattgtggg
     9481 tggtcgtcag ctccaccagt gggacgcccc ggccggtctg ggcgtcaacg acctggatct
     9541 tgaagtagtc cgcgggcgac agtaccgcca accggcagat cagcagcaac tggcacaaca
     9601 gcattgggtg gtgtcctccg aggcaatggg gacaactact tgcacttggc gagagttgta
     9661 cccgcttggg gcaagtgcgg caaggcgatt ggcggtcgcc ggcggttcgt tggtatactg
     9721 caacctcggt tttcggctga ggaggagcac ctgacgcatg aatatcttca tctccgccga
     9781 catggaaggt atctgcggcg tattcggcct gcatcaatgg gaatcaggcg gatccggtta
     9841 tgagcgggca tgcgagtgga tggcagcaga ggtgaacgcc gctattgaag gagccgtggc
     9901 ggccggtgcc cgtcggattg ttgtcaaaga tgcccattgt gacgggaaca acattgatct
     9961 ggaaaagctt catcctgcgg ctgagctgat cagcggctgg ggaccgctca acagcatggt
    10021 cgaaggcatc agccccgagt ttcacgccct gattctgatc ggctatcacg cccgggcagc
    10081 cacggccgac gccacgtttg cacatacctg gtcgcggaac gttctggaac tgcgggtcaa
    10141 cggaacgccg gtgggcgaag cggagtgggc ggcggcgttt gccggacatt tcggcgtgcc
    10201 ggtggcgatg atcacgggag atgacaagct ggccgaccaa gtgcgcgaga ccatcccggc
    10261 aggtttcgag tttgtggtga ccaagacagg ctggtccttc aacgccgccc agatgcggcc
    10321 gatcgccatg gttcgacagg aaatacgcga gacgaccgga agggttttgt cgcaaccacg
    10381 tcgtctaccc gtgtaccgcc catcgctgcc ggcaacgatc accgtacgtt ttcgccagtg
    10441 ggagggtctt cacgcctgcg aggcgctccc caacgtcagg cgactcagcg tggacacttt
    10501 cgaatacgcg gccgccgatt tcatcgaagc ccacaagtac ttctcgacac tgaatcgcct
    10561 ggccaagcct tgatcgacaa agcggaaagc gggctgatcc tgctttgcag cccaatgcca
    10621 agcaagccgc tgaaagagag cccgcctcgt gtgttgacga atcacatgga gacgccaccg
    10681 cgtaatcttg cagcgagggg caatccgcag gcgttcagcg catccagccg agttgtcgaa
    10741 gccctttctg gcaaaggccc tgcacgtcaa accagccaat cagcctctgc gttgcgtacg
    10801 gcagcgcccg ggaatgaaga atggcgaatg cagaattgag aacggggttt gttccccccg
    10861 agcggcgaaa tgcaccggca tagtgtttcc gttcgagcca agagtctcga gaggtagatc
    10921 gtctccatgg ctccttccga tctttcccgg tcggtcttat gcaaccggaa ggcaggaccg
    10981 tcccaagggc accaacgtcc gggcctgggc gctgatctgg gacacgttgc caggaggcgc
    11041 aaccatcgtc gcccgtgccg gcatcgacct cgccgccagc tgtctgtgcg gcctccgtca
    11101 gcataccggc cacgctccgg ccaatggcat tcggaggcac ggcactgatg aaaaggtgat
    11161 cctggcagcg agagcaagaa cccgcgaatg aggcgcgcct cgcgactgcc cgccacccgg
    11221 cggcaggcct gcgcagaccg ccatcggaca cgcgacgtgc ccgcgcgcga aatgcaagcg
    11281 ttcaggtcgg tcgagccgct gatcaggccc ttgcctcttc gcgggctttt ctggcgttct
    11341 cgatgatctg ctgctggatg ttgccgggaa cgttctcgta gtgcgacagt tccatagtaa
    11401 agctgccttg gcctccggtg atgctggaca gccgcgagtg gtagtcggac atctcggcaa
    11461 gcggaacccg ggcggaaatg acgctcaggc cgccagggag catttcctgg ccttccggcc
    11521 gaccgcgacg cgacgcgaga tcgccctgga tgtctcccac cttatcgttc ggaacggtca
    11581 cctcgacgtg cacgatcggc tccaggagca ccggcttgca ctgcatgaag gccttcttga
    11641 aggcctcgcg gccggcgatc tgaaacgcga tgtccttgct atcaacgggg tgcgtcttgc
    11701 cgtcagtcag ccgcactttc acgtcgacga ccggatagcc ggcgagcacg ccctccttca
    11761 tctgggcctg aatacccttg tccacactcg ggcggaacga caggtcgatc gctccgccaa
    11821 agatttcgtc aatgaactca tagcccgcgc cgcgctcgtt cggctccatg gcaatgatga
    11881 ccctggcgaa ctggcctgca ccgccggtct gcttcttgtg cgtgtactcg acgtccttgg
    11941 tgctgccggt gatcgtctcg cgataaggga tcttgggcgg cttggtattg acctcgagct
    12001 taaagtactg agccatgcgc gacagaatcg accgcaaatg caggtcgccc acaccgcgga
    12061 tcaccagttc gtgcgtcacc gtgtcgcgat cggcgatgaa gcaggggtct tcatcacagc
    12121 atcgagccag ggccccgctg accttgtcgg cgtcaccgcg actcttgggc tcaatggcca
    12181 acgagaacat cgggtgcggg aacttaggca tggcgatggt cccgccgccg gcctcgaatc
    12241 gcacgacgtc gccgatgtgg gcctcgattt tggccatggc gatgatgtcg ccggcaattc
    12301 cctcatcgat ctcggggtgc tcggccccct gcagcttgtg gagctgtccg ggccgctgcg
    12361 attttcgatc gtcgccaaca tagatctgcg aatcggactt gaggctgccg gcatggatcc
    12421 ggcagacgga atacttgata ttgctctttg ggtcactgaa gaccttgaaa acctgcccca
    12481 cgagcttctc gttcctgtcg ggcctgacgg gcgtttcagt ttcatcgacc ttgagaacgc
    12541 gctgtttgcc ttcgaggggt gaaggggcaa attcggcgat aacgtcaagg agttcagaca
    12601 cgcccacctc accgcgagcg tcggtgaaca ggatgggaac gaaggttccg gccacgatcg
    12661 ctttggctac tacggcgcca agcctggtca cgtcgacctc gccggtttcg agatactgct
    12721 ccatcatagc ctcgtcggcc tcgacgacgc gttcgatcag agcggtatgc gccgaggcca
    12781 catccagaat atccgcctgg ccgtcgctct gctggaaaca gtcgatcacc gccctaccct
    12841 ttttggccgg caggttcatc ggctggcact cgttgccgaa gagttcctgc acggcggcga
    12901 cgacgccggc gcagtcgggg ttggcctcga tcttgttgat cacgatcatt ctgccaaggc
    12961 cgtgctcctt ggcccgctcc atcatccggc gggtgttgac ttcgatgccc gccgtggccg
    13021 agataacgca aatggccgtt tcgacgccgg ccaaggctgc gatcgcgggg ccgatgaaat
    13081 ccggagcgcc gggggtatcc acaacgttga tctgcctgcc cttgtgggtg acgtgtataa
    13141 gggcggaatc aatcgagcaa cctcggttct tctcctcgtc catgaagtcg aggtagctgc
    13201 tcttggcatt gacgtcgccc aaccggttgg tcgtcccagt cgcatgaagg atggcgtcgc
    13261 ccagcgacgt cttacccgag gattggtggc cgacgagggc cacgttccgg atgtcagtcg
    13321 tactgtagtt tggcatctaa atctccgctc gcagcaaacg acccggtacc caccgtccgg
    13381 gtaacaaacg aattccaggt caagtcggca acagcccggc tattgtattg agattgatac
    13441 ttgccacaag ggcggactga gctgcgatcg agcatggggc cacaacagtc cggacggccc
    13501 gttccgaacc tcgagcgcga gcaaagggcc aggccgcaac ccgagcgctc tgcgatccag
    13561 gagcatgcgc gcaagccgca cagatgcctg gccgtaagat ttccgactct cgcggacgct
    13621 tcgagtattg ttgacgaatc cggcgggggc atggttcggg tttcaccggc ggccttgtcc
    13681 gcggcgatga gggaacgctc gcagacagcc cgttgttggc cccggccaag agtgtccgcg
    13741 ggtcttccag catccatgtt cttgacgctc atgggcacgg ccgtatagtg gcgaccggcg
    13801 agcctgggac aagccggctg gtgacccggg gcgcagactt caagccttcc ttttgtatcg
    13861 agagcaagac tcgcaatgtc atcaacagca atcgtttacg tcaacggcag agaaatcctg
    13921 gattcttgcg gcaacccgac cattgaagcc accgtcgtgc tggagaacga gtgtgtgggg
    13981 caggcctcag ttccgtcggg gaggtccaaa gccgcagatg aggcggtcga gcttcgcgac
    14041 ggggacatgg cgcgatacgg gggcatgggc gtactgaaag ccgttgaaaa catcaacgag
    14101 aggatcgccg aagccatctt ggggctcgat gcctgcgacc aggagtcggt ggaccgggag
    14161 atgatcaatc tcgatggcag ccccaacaag ggcaacctcg gggccaatgc catcctagca
    14221 gtctcgatgg cagtggccaa ggcggctgcc gaggcgtgca aactgccgct cttccgatac
    14281 cttggtgggg taagtgccca tgtcatgcca gtcccgatga tgaacatgct cgacgggggc
    14341 cggcgcgccg acaacaacgt ggatttccag gaattcctga ttcaaccgtg gggtgccccg
    14401 accttccgcg aagccttgcg gtggagtgcc gagatccagc aaaccctcgc aagtattctg
    14461 agcgaaaggg gttacagcgt ttccgtcggt gatgaaggtg gttttgcccc gaagctcaag
    14521 aacaatgatg aggctttcga gctgatggcc atggccgtgg agaaagccgg ctacaagctg
    14581 ggcgagcaga tcttttttgc cctggatccg gccatgagtg agctattcgg tgaggccaag
    14641 gccaagggca aggtagggta ttgcctcttc aagagcgatc cggaccggat cctcggcagc
    14701 gacgaggtga tcgactgctg gctggctcta tgcaggaaat ggcctcttcg cagccttgaa
    14761 gacgggctgg ccgaggacga cagggacggt tggaagaaac tgaccgccag cctgggtggg
    14821 aagatccagc ttgtcgcgga tgacctgtgt tcgaccctca tgaatcgcct tgggcgaggg
    14881 tttcgggaag ggatcgccaa cagcatcctg gtcaaaaccg accagatcgg gtgcctgacc
    14941 gaaacgctca acaccatcaa tctggccatg cgaaacggct atacgacggt ggtcagtcac
    15001 ggaagcggcg aaactgaaga caccacaatt gccgatattg cagtagcgac gaatgccggt
    15061 cagatcaagg ccggcggcgt cagacggagc gatcgggtcg caaagtacaa ccagttgctc
    15121 cgtatcgagg aacacctcgg agacgaggcc gtcttcggag gcacgttctg gaacaagtaa
    15181 cggccggaca gaggttcggg catggacgcc gacacttgcc caaccgagca ggacaaccag
    15241 accgctatcc accccgaaca gggcgcccaa ggcggcggtc gggcggacag ccccgggggc
    15301 gttgtcgccc agcattgcat catgttctgg gcgctgacgg tgctggccat ggccgttttc
    15361 gccccctgcg tgctggctcc catttatgct gaaaccgaac agatcatgga gacggaacag
    15421 aagctggaac ggctggtggc ggacctgaag gatcaggcaa atcgaaatga agagcggatc
    15481 aaggccatca aggcggatcc gcaggtcaac cagcggatca tccgccgcga actgaactac
    15541 cggtttgaag gggaacagat catccagtgg ccggcagcgg agttggccag cacaaggctg
    15601 gatctatcga tgctggaagg tgcacaggaa ccccttccgc cgatcaaccc ctatccggcg
    15661 tgggtgctga gactcaggca atggcttccg gcctggccgt ggcacaagct tttcgtcgaa
    15721 tctcccaacc gcgagctgct ggcgatcatg gccgccggac tgctggcgac ggcgtttttt
    15781 ctttatggtc cgctgccgcc gatcgccgga ccacaaagag acgatcgctg agcactcaca
    15841 agacatctgg gaaactcaca ggcgcctgac cccattactc acaccccaac cactcgcgaa
    15901 ggcttcggga ggtttgaccg tatggccgac ctgcctacca tggcaccccg caccaacgga
    15961 tgaacgctcg aatgggttga cgttcgcctc gcggcgtttg tttacgggga gctttggtca
    16021 tggcgtttga caggggatcg ttcagtttca agcggttttt cgtttcgggg cggagccccc
    16081 agcgtgtgga tgaggcgctg ctcggacaat tggcggccaa ggccatcggt gccgacagca
    16141 ttcagacggc cgaccacagc gaaatcgggt ggagcaccgg tgaacacatt ctcgacaccg
    16201 agttcgactt cgccaagaac gcagtggccg acggcttgta cttcgctctg cggatcgaca
    16261 ccaacaagcc accgaccgaa ctggttcgca gctaccagaa gatcaacgag caggcgatgc
    16321 tgcgagccac cggccgcgag ttcctcagca aggcccagcg ccgcgaggcc agggagcagg
    16381 ctctggcgcg cttggactcc gaggccaaga gcggcgcgtt caggcggatg aagcaaatcc
    16441 cggtgttctg ggatctgacg cgcaatgaac tctacttcgg ctcgaccagc aactcggcgg
    16501 ccgaatcgct gatgctgctg ttccgcgaga ccttcgaccg cggcgtcgtg gcggcctcgg
    16561 cgggcgagat cgcggcgcga tgggcggcgg caagcggcga aaaccggctg ttcgaggatt
    16621 gccggccggc tcattttgtg actccgcccg aaggggcagg cgagactccg gacgcgcatt
    16681 cagatgaagg ccgaacaaac gatttcctcg gcaccgagtg gctgacctgg ctgtggtatg
    16741 cgacgcaagt tgagtcttcc accataacga ccgccaagtc gggagcggtg accgtgctgt
    16801 tcgagaagtc aatgcagatg cagtgcgcgt tcaggatgag cgggtcggtg gcggtcagct
    16861 cagacagccc gacgcgtctg cccgaagccc tggtcgcttt atcaggcggc aagcagccag
    16921 tgcgggcggc tatgcagatc gaagcccagg gcagcatctt cagttttgcc ctccggggcg
    16981 atgcgatggc cttcagcggc gtgcagttgc cggcacctga ggatgccaac agcgtcaggg
    17041 cggtcttcga ggatcgtatg agcagactgc gcgatctgat cgacgccggc gacgggctct
    17101 acgccgcctt tctgaaacgc cggttgtcga gcaaatggcc acaaacgctc agcgccatgc
    17161 gggcgtggat cgcctcttgc cgacatgcgg cctcgtcgca ggacatgcag acaacggcga
    17221 tgctgggggc ggtttcataa ggcgaggggc caaaccgcaa atctgcaaca tcacaggaag
    17281 cgccgctccc ttgcggtcgc ggttctgaaa gaggcccccc ggccgggctg tcacggaccc
    17341 gtcgggcagg gcgggctctg ccggttgttg acaccagatt gatctgtttg tgccgttgtt
    17401 acaagtgtta tcatcataac caggctgacc gtcgcggagg gagcgcatcg ctcgcaggac
    17461 gatccggctt tttgggtagc ctgagaagag gcacggacct cggtgcatca gtcgcccagg
    17521 agattgacgg catgtcacaa gtgacagggc cggcttacaa acacgaggac tacgccggtt
    17581 tcatccgccg gacggcagcg ctgcttttcg acatcattct ggtgatcctg gcgtccatct
    17641 taataggcgg aatctgcatt tccatctcat cgagtccatg gactgaggag caggaggatg
    17701 cggttctcgc cttcgtgttc acgatagctt tcgtgttgta catgctcggc ttccgcctgt
    17761 cactgaaggg cacaccgggc tatcgactct tcagaatccg ctatgcctat atgctggacg
    17821 gcaaaccaac ggctttggtc cttctgttcc gatcgttcgt ggcggtggtt cttctgctgt
    17881 tcttcgcact cgatcatctg tggattctgt ttgacgagca caaacaggca tggcacgaca
    17941 agctgagcgg gttctacgtg gtcaagagca aggctcggcc gtgcgggaag cagcgggtga
    18001 cacggcgaat catcaatttc atgatgttga cgttcattgt ctgggaagcc ggtcctgaga
    18061 ctcatgccgg gacagcagca ggggcgacgt gacggattgg caggttgaga cgaggcgcgc
    18121 tgaaccgccg gcttgcatac cgcaatggca aggaatggag gatagcgatg caacgttttc
    18181 tggcaatgtg tttgttgggt tgcggagcgg cgacgttcgc ggcggcggat acactggcgg
    18241 cggcgcgaac cgagaagaag cccgacatac ggacccagct cgaacaagaa ttcgaggcat
    18301 tgcatgagag cctcaagaag gccatgccgg aggatcccaa gacgctgcgc gatccggccg
    18361 tccgggccaa ggtcgccccc gccgcgatgc ccattcttca gaagacgatt gaatgcctcg
    18421 aacgacgggc caggtttgac aaatcattgg ccggcgactc ggcgtggtac cgagtcatcg
    18481 ccttggccct gggcaacgat tcgatcaaga ccaacctgac gacagccgcg gagagcggcg
    18541 accgcggggc caagatccaa ttggccgctt cgagggttat tgtggccgcc gacacctcac
    18601 aacggaaaac cgcgatgaac gaattcgccg ccctgctggg cgccgagggc gctgctccac
    18661 ccggggcggc gacggctctt caggcggtgg tgtacgcggg agccctttcc aaggaggaaa
    18721 tcaagcagat ctccgacgtg gtcgagaagc ttcctgacaa gactctgaag aacgccctgg
    18781 atctggcaac gatgaccccg gccgagcgac gtgcggcgtt gaggggcaag ccgctggtgc
    18841 tggagggtaa actgctgacc ggcaagccgt tctccacgga ttcgctcaag ggcaaggtga
    18901 ttctggtgca cttctaggcg agctggtgtc ccgcctgtcg cgagtcactg ccgcaggtga
    18961 aagaggtccg aaacatgtac gggaaaaagg gcctcatggt tgtcggggtc tgttgcgaca
    19021 gggaagttcc ggccttgcgg aagttccttg ctcagaaccc ggatgtgaac tggcctcagc
    19081 tcttcgacac ggcgaaaccg ggttggcacg aacttgcccg gcagcacggt atcgacgtaa
    19141 tccctgcgat gttcctgatc gacaggaaag gcatcgttcg aagcgtcaac gctcagcgcg
    19201 agatgaagaa gctggtaccc atattgctcg acgagtgaag tcggccggca tgaatgacag
    19261 ggtcaggcct gcggcaggtc ggcgccctac tccgtcgggg cgacttggcg gggctgtgac
    19321 ccgctggtcc cagattccca tctggga
//
LOCUS       DAOTPW010000099        19268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2901_length_19268_cov_5.532452, whole genome shotgun sequence.
ACCESSION   DAOTPW010000099 DAOTPW010000000
VERSION     DAOTPW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19268
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2901_length_19268_cov_5.532452"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="P5316_10595"
     CDS             <1..192
                     /locus_tag="P5316_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85445.1"
                     /translation="FDWNRMTRYYRAARRFAIHKHYPNSNVLLSDNDDEVDVAPAPRL
                     MKAPEKKRKHIGGSAPPKA"
     gene            complement(273..1052)
                     /gene="lptB"
                     /locus_tag="P5316_10600"
     CDS             complement(273..1052)
                     /gene="lptB"
                     /locus_tag="P5316_10600"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRR85446.1"
                     /translation="MPKTRNLLTTIDLIKRYGARTVVDRVSYSVGQGEIVGLLGRNGA
                     GKTTSFRITIGMITPDGGQVLFNGEDLASLPMYKRAQRGIGYLSQEPSVFQRLTVRQN
                     LLAILECMKLGRRERNLRADALLEQFGLRHLEGQLARNLSGGERRRLEIARALVTEPL
                     LILLDEPFSGVDPIAVEGLQKEIIRLRAEHGIAILLTDHNVRETLSVTDRSYIIHEGQ
                     VIAEGAPRDLINDSKVREIYLGSTFRGDEFDHPRYHAGTTG"
     gene            complement(1361..3235)
                     /locus_tag="P5316_10605"
     CDS             complement(1361..3235)
                     /locus_tag="P5316_10605"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRR85447.1"
                     /translation="MAPNKTAAASDPRNVRPAPRPAPGQRAATIEEAERKSLAVTVKE
                     TLESIVVAFILAFVFRAFIVEAFIIPTGSMAVTLYGKQLTKTCSTCGYEYAYGLVDGR
                     PIAETRVRCPNCRALYDKFSAADLERPDSGDRILVHKWPLDLPGGWLGPDRWDVTVFK
                     DPEDGTTNFIKRLVGEPGEALEIIDGDVYAAKMDNLPAELIEQFEQLRKLVYRYGHGD
                     RQSDSSDAREEIRRLFAEINKKLLPRLEIQRKIDQAPRAQESLWFNVYNHDYLPRYQL
                     RDDQNKPRVGWVPDSPDAAKAWKDTDRRTIKFDSVSDEILYLKFEGTIDDFYAYNFSD
                     SANEAMDLRGGPSSGRCPVGDVRLRFSWVVRAGSGDLLLEMNRDKDKFIASISTTGRV
                     KLELERPVGGTATRRETIAETAPTRSFSRDRAVQVEFMNVDYRAMVLIDGKIVAKSND
                     KQYHPVIERLERFCHPTPTDATWGTVEPTTVRIGGRNMQCDLKHLVLERDVYYRSQPL
                     LGAPWKGGSLRLHDWAGWGTAGHPILLRPKRVRDGKTFEAEYFMLGDNSPHSKDSRLW
                     ETAGSHLIPLEDEYQLGTVPEDQLIGKAFFVYWPAGYRAKWTAGIGLIPNFGRMRWIR
                     "
     gene            complement(3393..5195)
                     /locus_tag="P5316_10610"
     CDS             complement(3393..5195)
                     /locus_tag="P5316_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRR85448.1"
                     /translation="MSAGYPVFVKRDLDGFFGLAIDNLVQLLLIVSLCTTVCGMTGDN
                     ARFIYEYIMPGAAVSLLIGNLFYAAQAHWVARRERRSDVTALPYGINTPSLLVYVFFV
                     MKPAFDRTGSAEYAWKLGLIACLGSGLIEFAGAFVAERLRRNTPRAALLSTLAGIAIT
                     FIAMKFALEIWDRPLVAMVPMAVVLLVYFSQVRFPLGLPGGFVAVLLGTALAWVLGSL
                     GVSTGVPVSTEAVREACAYSGRHWPQWVGPVLWDTIRSDTAQVISLLSVIIPMGLFNV
                     IGSLQNIESAEAGGDVFATGPSLAVNGIGTIAAACFGSCFPTTIYIGHPGWKALGARA
                     GYSTLNGLVMTILCVTGTVAVVSKIVPMEAGIAIVLWIGIVITAQAFQATPVRHAPAV
                     ALGLFPAIAAWGAMIVSGAFTQTGLNTVNAATTAPAAVITSLPVTESLPAPATQLSQW
                     PGVATIQSILSSEDGSKMETSGFLVHGMNIMERGYIFTCMIIAAISAFLIDRRFFHAA
                     GWSLAAAVLTILGLMHAYQMVGNTADYWLIGTTPAEGAFTYHAYPVVIGYLLMAAVFV
                     IMGYRHRHEAVGTAKTLEDARVVTPGSLSGECFI"
     gene            5438..6214
                     /gene="kdsB"
                     /locus_tag="P5316_10615"
     CDS             5438..6214
                     /gene="kdsB"
                     /locus_tag="P5316_10615"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRR85449.1"
                     /translation="MSVAAIIPARYASTRFPGKPLANQTGKPLIQHVYEAVRGARRVE
                     RVLVATDDLRIAEAVRSFGGEAVMTRADHPSGTDRVAEAASRLDADLIINVQGDEPEM
                     DPQSIDTLVELMEQRPQAQMGSLACRFKRAEDVLNPACVKVVLDANGYALYFSRSVIP
                     YPRDARGQIEQPANWLLHLGIYAFRPDFLARLTQMPPAELERLEQLEQLRALTMGARI
                     AMAVVPHESNGVDTPEQYSAFVERYRAARVQAGTLPTGKG"
     gene            6330..8039
                     /locus_tag="P5316_10620"
     CDS             6330..8039
                     /locus_tag="P5316_10620"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRR85450.1"
                     /translation="MPAGYRPGRTKYVIVFGTVMSGLGKGIFSSSLAKCLQDKGLTVA
                     PIKLEGYLNIDSGTLNPYRHGEVFVLDDGMETDMDLGTYERMLDQDLTRMNFATSGQI
                     YSRVLEKERQGSYLGRDVQMIPHVTGEVKSKLRELAVTSDADVVFVEIGGTVGDVENS
                     YYIEAMRELAFEEGPDSCTFVALTYIISPPMLGEQKSKAAQLGIRGLLATGIQPHIIA
                     CRGKEPISQKVREKLALYSNVPIGNVFSMHDCASIYVIPEMLREAGLDEVVIDTLRIR
                     GRLHPEGEKKACAIWGEFLNRIQSPSREVTIGLIGKYTSVRDSYASIIHALEHAGAAA
                     DCHVNIKWVDTTELDPEKAARAIGDVHGIIVPGGFGVRGTEGKIGCIKCAREKGIPYL
                     GICYGFQMAVIEYARNVCGLQDANSTQIAPDTPHPVIDILPEQKQIEGLGGNMRLGGR
                     DVEIKPNTLAARLFNGAREVRLRFRHRYEVDPQYIERLEAGGLIFSGKAPDYPIMQIL
                     ELPQDVHPYFIATQAHPEFQSRPLRPQPMFLGLVKAALAKAQSSDTGRTTAVDATKVN
                     AGT"
     gene            complement(8365..9894)
                     /locus_tag="P5316_10625"
     CDS             complement(8365..9894)
                     /locus_tag="P5316_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRR85451.1"
                     /translation="MLSRLHSVALMGIEALPCEIEVDCAEHGFEKAAIVGLPDAAVKE
                     SLERVRAALNNSGYDWPRYKTVVNLAPADVKKEGPAFDLPIALGMIFANGQGTPEPAG
                     QYLIAGELALDGRVRPVKGALSMAILARDKGFNGLILPRENAPEAAVVEEIEVIGIGT
                     LAEAVGFLTGQLPLEPTVIDINAVFADASRYEEDFSEVRGQESVKRAVTVAAAGGHNI
                     LLIGPPGTGKTMLSKRIPTILPPLSLAESLETTRIYSAAGKMPAGQALLARRPVRSPH
                     HSASGPALVGGGSIPQAGEVSLAHHGLLFLDEFPEFPRTILETLRQPLEDGVVTIARS
                     HSAITFPARFMLVAAMNPCPCGYFTDPNKPCKCSPLQIDKYLSRISGPLIDRIDIHIE
                     VPPVPYRELRGEHAGSSSPEMRAQVERARRIQRERFGDTSTILNARMTPKQLRRYCRL
                     DAAGEQLLKQALTELGLSARAHDKILRVARTIADLAGEENIRPEHVSEAIMYRRLDRQ
                     L"
     gene            complement(9959..10357)
                     /locus_tag="P5316_10630"
     CDS             complement(9959..10357)
                     /locus_tag="P5316_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRR85452.1"
                     /translation="MSFLVDTDVCSAHLKAKGVVTNRFLQYAGRLHISTVTLGELYAW
                     ALRAEAPPARLRGLREMLADVTVLEVTARIAERFGQVQAGLLDARRPAPAMDLLNGAV
                     ALVHDLTLMTHNVQDYAGIPGLRVVDRTVP"
     gene            complement(10354..10608)
                     /locus_tag="P5316_10635"
     CDS             complement(10354..10608)
                     /locus_tag="P5316_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85453.1"
                     /translation="MSSESVTTRGIARGKTIELEREPGLPDGQQVKVTMEPADKESDL
                     SPGEGLRRSAGAWADDAEGLDRYLEWNRQQRKLGRRERPE"
     gene            complement(10689..10877)
                     /locus_tag="P5316_10640"
     CDS             complement(10689..10877)
                     /locus_tag="P5316_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85454.1"
                     /translation="MLSKLHSAAFMTIEALPREIEVDCAEHGFEKAAIACPGEAYAPS
                     GTRGETCGVAGSACPMRP"
     gene            complement(10960..11388)
                     /locus_tag="P5316_10645"
     CDS             complement(10960..11388)
                     /locus_tag="P5316_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRR85455.1"
                     /translation="MSVVVDASVFVAASRPSEAHYRESVEFLVMLRREAETICCPVLV
                     LAECSGAIARMTQSSALAGRIVRLIEGIENLQMIPLTLALAKQAAQLAGDHRLRGADA
                     VYVAAASELKAALVTWDDEMLQRSPRTVSAITPAQWIRKA"
     gene            complement(11385..11582)
                     /locus_tag="P5316_10650"
     CDS             complement(11385..11582)
                     /locus_tag="P5316_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85456.1"
                     /translation="MTYKGIVKHGKIELEEGMPLAEGTPVRVEAEPADWQSEWEAFAR
                     EVDREWKSPLSALEALRESRR"
     gene            12719..12916
                     /locus_tag="P5316_10655"
     CDS             12719..12916
                     /locus_tag="P5316_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRR85457.1"
                     /translation="MAEYKVVRRCRSDRMIAGVCGGLADFFGLSSANVRILYVAVSVL
                     SAAFPGIIVYLILWLLMPLED"
     gene            complement(13422..13583)
                     /locus_tag="P5316_10660"
     CDS             complement(13422..13583)
                     /locus_tag="P5316_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85458.1"
                     /translation="MRSRLSSVALMGIEALTCRVEVNCAEHGFEKAAIVGLPLPPTEP
                     FRAGSGLGL"
     gene            14137..15078
                     /locus_tag="P5316_10665"
     CDS             14137..15078
                     /locus_tag="P5316_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85459.1"
                     /translation="MIHLSVSTRTTMWGLAAILCFLTLAGAAQAAPLLCWSMHDGIRT
                     SNLDGTNINTWLSKPNYPIFAEVHDSPAVGKVYWFESGGYIKRADYDGGNAELVITDT
                     KMYTTAGDFAINRLNGDVYWANVNGELWRAGPDGSNKQLVRSGLASSGAIEIDPIHQK
                     LFWLQYSVGTDIYMGDLNGGSATYLTSVSSLWKVTDLAVDTQSSRLYWSETGVTVNER
                     VRRLGLGPGTPSVIVDAELGYLNDVTLDLDSGRLLLVDRSGDRILQCGLDGSGLTTLI
                     SGEDEPQHLTILPVPEPATMVLLGLAGAAVLYHRRRF"
     gene            complement(15542..16768)
                     /locus_tag="P5316_10670"
     CDS             complement(15542..16768)
                     /locus_tag="P5316_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRR85460.1"
                     /translation="MNRYIVPSLTTLSENTCLSAIRAGMMTVVPLTIIGGLFMIVSFL
                     PVKGWNDRVAPCLPLLQIPVTATFGLLAVFACFAVAYDYGKRLNQEAIVSAAMAALVF
                     LMVQLKPDDQGFSMDGLGSKGLFTAILIALITVRVQKWFTDSNLVVKLPRNVPPVVYE
                     SFLSLSPMFFLVVVFWLIRFVAGLDINHLLQTVLTPLVFALNTLPGILVYAFLVTLLW
                     SVGINGDNTMDAAVAPVFLQYLAANVEAASRGQPLPYVTALGFFTSFVNVGGTGATIG
                     LALVMLRSKEPAFRKISRLSLPTQIFGINEPIFFGFPVVLNPILMIPYILNALILTTG
                     SYLLMSCGVIQKPFVAVPWTTPPVIGHYLVSGGDWKAAVWGVVSIGLAMLVYYPFAKA
                     AERRRLHAETKSQARE"
     gene            complement(16786..17409)
                     /locus_tag="P5316_10675"
     CDS             complement(16786..17409)
                     /locus_tag="P5316_10675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR85461.1"
                     /translation="MNRFTIRSALPGDEAGAYYVCLKTGDNGKDGEPLYRDDPDALGR
                     IFVGPYLAFEPELSLILEDEQGICGYALAAFDSRSFYARYEAEWRPQLCARFPEPQGD
                     PGRWTRAQQVHYCYHHPDYFCPEPYAVFPSHLHIDLLQRARGLGFGRRMMEQVMNRLR
                     ERGSPGAHLGVSTLNTPALGFYEKLGFHELVRVGGDKDGCIYLGKRL"
     gene            complement(18041..18892)
                     /locus_tag="P5316_10680"
     CDS             complement(18041..18892)
                     /locus_tag="P5316_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85462.1"
                     /translation="MSSRIRSGLLVTGASIMVAGTMSVIAILSITPVRAADEAAKPTI
                     HICGIRVVGTGFKDKESEQQPFNQPQGTTLAILVRQPSGELIGFDEQESVLSAFTNDK
                     GDSLLDKKESFDEGFGSFPQVSKDGKAAIIELSAKRLPGKGARELRAKGSLAFKTGTQ
                     KKTAVEKAVALKKGTTFSVGPIEITIKQTGKPQWGDEPLEVTFEMGKAGDSLAGVRFL
                     DDKGTPIKSSASSSASMRLAGMVKLNKSYRLARQVETATVEVTYWTDMKTVRVPFDLT
                     VGLGSVE"
BASE COUNT         3803 a         5784 c         5985 g         3696 t
ORIGIN      
        1 ttcgactgga accggatgac ccgctactat cgggccgccc gccggtttgc cattcacaag
       61 cattacccga acagcaacgt actgctgtcg gacaacgacg atgaggtcga cgtcgccccc
      121 gccccccgcc tgatgaaggc accggagaag aaacgcaagc acataggggg atcggcacca
      181 cccaaggcgt aagcccggct tcgttctgcg gtgcctgctt gtgcaacgga acggtccaac
      241 gatcggtcat gcgccgccat ggcgcggccg gcctatccgg tcgttccagc gtggtacctg
      301 ggatggtcga actcgtcgcc gcggaaggtg ctgcccaggt agatttcccg gactttggag
      361 tcgttgatca ggtcgcgagg cgctccctcg gcgatgacct gtccctcatg gatgatgtag
      421 cttcggtcgg tgacagaaag cgtctcccgg acgttgtggt ctgtcaggag aatggcgatg
      481 ccatgctcag cccgcaggcg aatgatctcc ttctgaagcc cctcgacggc gatcgggtcg
      541 acgccgctga acggctcgtc cagcaggatc agcagcggct cggtcaccaa ggcccgggcg
      601 atctccagac gccgtcgctc gccgccggac agattgcggg ccagttgacc ttcgaggtgt
      661 cgcagtccga attgctcaag cagcgcgtcg gcgcgcaggt tgcgctcacg gcggccaagc
      721 ttcatacact ccaggatagc cagcagattc tgtcggaccg tcagccgctg aaacacgctg
      781 ggctcctgag agagatagcc gataccccgc tgtgctcgct tgtacatggg taaactggcc
      841 agatcctcac cattgaacaa cacctgcccg ccgtcaggcg tgatcatgcc gatggtgata
      901 cggaaacttg tggttttgcc cgccccgttt cgccccagca ggcccacgat ttcgccttgc
      961 cctacggagt agctgacgcg gtccacaacc gtccgcgcgc cgtagcgttt tatcagatcg
     1021 atggttgtca gcaggttgcg agttttaggc atcaccacct cggcagaatc gctgtcgggt
     1081 tgtcaccagt tttccttgtg gatagcctgt tgaaaagccc tgcgtccacg acgaaaaacc
     1141 gacgaggcgc cttgcttttc tgatacaaaa accctgtgcc ctttttcggg gcaggaagac
     1201 aacagcgtac aagcatcttc ctgacaaggt tttagggggc tatgcatcac ctgttaatca
     1261 gctcccgcgc tcagatttaa tcacagccag actcacccag aaggaattaa agtcatggca
     1321 atttcacgcc agttgtgtgg ataactatgc gggttatccc ctaacgaatc catcgcatcc
     1381 ggccaaaatt gggtatcaaa ccgatccccg cagtccactt tgcgcgatat cccgccggcc
     1441 agtacacgaa gaacgccttg ccgatcaact gatcctccgg aacggtaccc aactgatatt
     1501 catcttccag ggggatgaga tgactgccgg cggtctccca aagcctggag tccttgctgt
     1561 gggggctgtt atctccgagc ataaagtact cggcctcgaa cgtcttgcca tcccggactc
     1621 gcttggggcg caggagaatc ggatgtccgg ccgttcccca gcccgcccag tcgtgcagcc
     1681 gcaagcttcc acctttccat ggagcgccga gaagcggctg ggatcggtag tatacgtctc
     1741 gctccaacac gagatgcttg agatcgcact gcatgttcct gccgccgatc cggacggtgg
     1801 tgggttcaac cgtgccccag gtcgcgtccg taggcgtcgg gtgacaaaac cgttccagcc
     1861 gctcgataac cgggtggtat tgcttgtcgt tgctcttggc cacgatcttg ccatcaatga
     1921 ggaccatcgc gcggtaatcg acgttcatga actccacctg gaccgcccgg tcgcggctga
     1981 acgaacgcgt gggtgccgtc tctgcgatgg tttctcgccg ggtggccgtt ccgccaaccg
     2041 gacgttccag ttcaagcttg actctgcctg tcgtgctgat gctggcaatg aacttgtcct
     2101 tgtcgcggtt catttcaagc agcagatcgc cactgccggc tcggacgacc caactgaacc
     2161 tcagccgcac atcgcccacc ggacatcggc ctgacgaagg accgcctcgc aaatccatcg
     2221 cttcattcgc gctatcactg aagttgtaag cgtagaagtc atcgatggtt ccctcgaact
     2281 tgagatagag aatctcatcg ctgacggagt caaacttgat ggtgcgccgg tcggtatctt
     2341 tccatgcctt ggcggcatcg ggactgtcgg gcacccagcc cactcggggt ttgttctgat
     2401 catcccgcaa ttggtagcgt ggcaggtagt cgtggttata cacgttgaac cagagcgact
     2461 cctgcgcgcg cggtgcctga tcgatctttc gctgaatttc aaggcgaggc agaagcttct
     2521 tgttgatctc ggcaaagagt ctgcggattt cttcacgggc atccgacgag tccgactgcc
     2581 tgtcgccgtg gccataccgg taaaccagtt tgcgaagctg ctcgaattgt tcgatcaatt
     2641 ccgcaggcag gttatccatt ttggcggcgt agacgtcgcc gtcgattatt tcgagagcct
     2701 cgccgggctc gccaacgagc cgtttgataa aattggtcgt gccgtcttcc ggatccttga
     2761 acacggtcac gtcccagcgg tccggaccga gccaacctcc tggaagatcg agcggccact
     2821 tgtgaacaag aatgcggtcg ccgctgtcag gcctttccag gtcggcggcg ctgaacttgt
     2881 cgtacaaagc acggcaattc gggcatcgaa cccgcgtttc ggcgatgggc ctgccgtcaa
     2941 caagaccgta ggcatactcg tagccacagg tggagcaggt tttggtcagt tgcttgccgt
     3001 agagggtgac ggccatcgac ccggtcggaa tgatgaaagc ttcaacgata aaggcgcgaa
     3061 agacgaacgc caggatgaat gccacaacga ttgactcgag cgtctccttc accgtgacgg
     3121 cgagactctt acgttcagcc tcttcaatgg tggctgcccg ctggccgggg gcggggcggg
     3181 gcgccgggcg gacattcctg gggtcggaag cggcagcagt cttattcggg gccatcaatc
     3241 agtctcgaca aacctcagat cgtccagtac accggcaacc gggtgcttac gtccgcctcc
     3301 ggtgtccagt tcacgggcaa gccgaacggc aagaggttaa ccgctgccct ccaaagcgtc
     3361 aaatcactgt cagaaacaga cttatcgtgt tgtcaaatga agcactcccc ggataaactc
     3421 ccaggcgtca ccactcgggc gtcctcaagc gtcttggccg tgccgactgc ctcgtgtcgg
     3481 tgccggtaac ccatgatcac aaaaaccgct gccatcagca gatagccgat gaccacagga
     3541 taggcatggt aggtgaaggc tccctcggcc ggggttgtcc cgatcagcca gtagtcagcg
     3601 gtgttgccca ccatctggta ggcgtgcatc aggccgagga tcgtcaacac agcggcggcc
     3661 agcgaccagc cagccgcgtg gaagaatcgc cggtcgatca ggaacgccga aatcgccgcg
     3721 atgatcatgc aagtgaagat gtaaccgcgc tccatgatgt tcatgccgtg gacgaggaag
     3781 ccgctggttt ccatcttgga gccatcttcc gaactcaaga tgctctggat ggtggcaacg
     3841 ccgggccact gcgaaagctg cgtggccggt gcgggcaggg actcagtcac agggaggctg
     3901 gtgatgaccg ccgcaggtgc cgtggtcgca gcgtttacgg tattcaagcc ggtttgggta
     3961 aaggctcccg agacgatcat ggccccccac gcagcgattg cggggaacag gcccaaggcc
     4021 acggccggcg catgccgcac cggcgtagcc tggaaagctt gagccgtgat gacgatcccg
     4081 atccaaagga caatcgcgat gcctgcctcc atagggacaa tcttgcttac gaccgccact
     4141 gtcccggtca cacaaaggat ggtcatcacc agaccgttca aggtggagta gcccgcccgc
     4201 gcccccaatg ccttccagcc aggatggccg atgtaaatgg ttgtcgggaa acagcttcca
     4261 aaacatgccg ccgcgattgt cccgataccg ttgaccgcca gcgacggacc ggtggcaaag
     4321 acgtcgcctc ctgcttcggc tgactcgatg ttctgcaagc tgccgatcac gttgaacaac
     4381 cccatcggga tgatgacgga aagcagcgaa atgacctgag cggtatcgct gcggatcgtg
     4441 tcccacagaa ccggtccgac ccattgaggc cagtgccgac ccgagtacgc gcaggcttcc
     4501 cggactgctt ccgtgctgac tgggacgcct gttgatacgc ccaggcttcc caaaacccag
     4561 gccagagcgg ttccgaggag taccgccacg aaccccccgg gtagcccgag ggggaagcgc
     4621 acctgcgaga agtagaccaa cagcaccacg gccatcggga ccattgccac cagcggccgg
     4681 tcccagattt cgagggcgaa tttcatggcg atgaaggtga tagcgatgcc ggccagggtg
     4741 ctcagcaagg cggctcgggg ggtgtttcgg cggaggcgtt cggccacgaa tgcgccggcg
     4801 aactcgatca agccgctgcc gagacaggcg atcaggccga gtttccaggc gtactcggca
     4861 ctgccggtgc ggtcgaaggc cggtttcatc acgaagaaga cgtacacgag cagggaaggc
     4921 gtattgatcc catagggtag ggcggtgacg tctgaccgac gttcacggcg ggcgacccag
     4981 tgtgcctggg cggcatagaa gaggttcccg atgagcaacg agacggctgc ccccggcatg
     5041 atgtattcgt aaatgaatct ggcgttgtcg ccggtcatgc cgcagactgt cgtgcacaag
     5101 gatacgatca ggagcaactg gaccagattg tcgatcgcca agccaaagaa cccatcgaga
     5161 tcgcgtttga cgaaaacggg ataaccggct gacatattcg gtcctcgcat caagtgagaa
     5221 gtccagggcc tgcctcggcc gcggaaccta gcggtgatcc tacggcgcgc gggtgccact
     5281 gacaagacgc gcagaaactc acaggcacca gggtcttctg ccgaacggat cgcggactca
     5341 cggccttacc gctttgacaa accgcatgac ggccgctatc ctttgtgctt gttccgatga
     5401 gagtacgttt tgcctgcaga agaggaagtg ctccccggtg tccgtagccg cgatcatccc
     5461 cgcgcgttac gcgtccacac gattcccagg gaaacccctc gccaatcaga ccggcaagcc
     5521 tctgatccag cacgtctacg aagccgtgcg cggagctcgg cgtgtggaac gcgtgctggt
     5581 ggcgacggat gacctgcgaa tcgccgaggc ggtgcggtct ttcggcggtg aagcggtgat
     5641 gacccgcgcg gaccacccaa gcggcacgga ccgcgtcgcc gaggcagctt cgagactcga
     5701 tgctgacctg atcatcaatg tgcagggcga cgaaccggag atggacccgc agtccatcga
     5761 cacgttggtc gaattgatgg agcagcgtcc gcaggcccag atgggctcac tcgcctgtcg
     5821 cttcaagagg gctgaggacg tcctcaaccc cgcttgtgtg aaagtggttc tcgacgccaa
     5881 cggttatgcc ctgtatttca gtcgaagcgt gattccttat ccgcgtgatg cccgcgggca
     5941 aatcgagcag ccggccaact ggctgctgca tctggggatc tacgctttcc gtccggactt
     6001 cctggccagg ttgacccaaa tgccgccggc ggagctggag cggctcgagc agcttgaaca
     6061 actgcgggcc ctgaccatgg gggccaggat cgcgatggcg gtggtcccgc acgagagcaa
     6121 cggcgtggac acgccggagc agtattcggc ttttgttgaa cgctatcggg cagcccgcgt
     6181 tcaggcggga accctgccca caggaaaagg atgagcagca ggacaaggac gcgaatatga
     6241 accccgaacg taaaggcaag acggttgatg tggaaaagat gctttcctcc ctgagttaca
     6301 cctccgatga caccgagttc tacgccccca tgcccgccgg ctacaggccc ggtcgcacca
     6361 agtatgtgat cgtcttcggc acggtcatga gcggcctggg caaggggatc ttctccagct
     6421 cgctggccaa atgcctccag gacaagggtt tgacggtcgc gccgatcaaa ctcgaagggt
     6481 atctgaacat cgattcgggc acgctgaatc cctaccgcca cggcgaagtt ttcgtcctcg
     6541 acgacggcat ggagacggac atggacttgg gtacctacga gcggatgctc gatcaagacc
     6601 tcacgcggat gaacttcgcg accagcggtc agatctattc ccgcgtgctg gaaaaggagc
     6661 ggcagggcag ctacctgggg cgcgacgttc agatgatccc ccacgtcacc ggcgaggtca
     6721 agagcaagct tcgggagctg gcggtgacgt ccgacgctga tgtcgtcttc gtcgagatcg
     6781 gcggaacggt cggggacgtg gaaaactcct actacatcga ggcgatgcgc gagttggcct
     6841 tcgaagaggg gcccgactcg tgcactttcg tcgccctcac ctacatcatc tcgccgccga
     6901 tgctgggcga acagaaatcc aaggccgctc agttgggcat cagggggctg ctggccacgg
     6961 gcatccagcc acacatcatc gcctgccgcg gtaaggaacc gatcagtcag aaggtcaggg
     7021 agaagctcgc cctgtacagc aacgtgccca ttggcaacgt cttctcgatg catgactgcg
     7081 caagcatcta cgtgattccc gagatgctgc gtgaagccgg tttggacgag gtggtgatcg
     7141 atacgctcag gattcgcggc cggctgcacc ccgaaggcga gaaaaaagct tgcgcgatct
     7201 ggggcgaatt cctgaatcgc attcagtccc cgtcgcggga ggttaccatc ggtctgatcg
     7261 gcaagtacac gtccgtgcgt gacagctacg cgagcatcat ccatgccctg gagcacgcgg
     7321 gcgcggcggc ggactgccac gtgaatatca agtgggtcga cacaaccgaa ctggatcccg
     7381 agaaggccgc gcgggcaatc ggtgacgttc acggcatcat cgtcccaggc ggattcggcg
     7441 tgcgtggcac cgagggcaag atcggctgca tcaagtgcgc ccgtgagaag ggtattccct
     7501 acctgggaat ctgctacggt ttccagatgg cggtgatcga atacgcgcgc aacgtctgcg
     7561 gcttgcagga cgccaacagc acccagattg ccccggacac tccgcacccg gtgatcgaca
     7621 tcctgcccga gcagaaacaa atcgaggggc tcggcggcaa catgcggctt ggcggacgag
     7681 acgtggagat caagcccaac acgctggccg cccggctgtt caatggggcg cgggaagtcc
     7741 gcttgcgatt ccgccatcgc tacgaggtgg atccgcagta catcgagcgg ctcgaggccg
     7801 gcgggctgat cttctcgggc aaagcccccg actacccgat catgcagatt cttgaactgc
     7861 cgcaggacgt tcacccctat ttcattgcga cccaggcgca tcccgagttc caaagccgac
     7921 cgctgcgccc gcagccgatg tttctcggat tggtcaaagc cgcactggcc aaggcgcaat
     7981 catcggatac cggccgaacg accgcggtcg acgcgacaaa ggtcaacgcg ggaacataag
     8041 agtccgggag gagggagtca gggagtctaa ggccttgcgg ccggagatct gtgcggcttg
     8101 cgcgcatgat tgcagatcgc agtgcgctgg ggttgcgcgc cgcaagaagg cggtcggcct
     8161 tgggattcgc ctgccgctct ttgacgaaga ccccggaagt cgcgattgaa acgagtgcgc
     8221 ggcgaacatc agtgagaacg aattgaccgg cccgcaactc gtcgaggaag aagcgagcca
     8281 cctcaacccg ttcgccctca aaggcatctg caattgccgg tcgcagcgca tccgtatcct
     8341 ttacagcgta ccggtgggtc catttcacaa ctgccggtcg agccgccggt acatgatcgc
     8401 ctcgctcacg tgctcaggcc ggatgttttc ttccccggcc aggtcggcga tcgtccgggc
     8461 gacgcggagt atcttgtcgt gggccctggc ggacagaccc agctcggtga gggcctgttt
     8521 gagcagttgt tctcccgccg catcgagcct gcaatacctg cggagttgtt tgggcgtcat
     8581 gcgggcgttg aggatggtgc tcgtgtcgcc aaagcgttct cgctggatcc gcctggcccg
     8641 ctcgacctgg gcgcgcatct cggggctgct gctgccggcg tgctcgccgc gcagctcacg
     8701 gtaaggcacc ggcgggactt cgatgtggat gtcgatgcgg tcgatgaggg ggccgctgat
     8761 ccgcgagagg tacttgtcga tctgcagcgg gctgcacttg cacggcttgt tcgggtcggt
     8821 gaagtagccg cagggacaag gattcatggc cgcgacgagc atgaagcggg cggggaaggt
     8881 gatggcggag tgcgagcggg cgatggtgac cacgccgtct tccaggggtt gacggagcgt
     8941 ctccagaatc gtccgcggga actcggggaa ctcgtcgaga aacagcagcc catgatgagc
     9001 cagcgagacc tcgccggcct gcgggatcga gccgcccccg accagggcgg gtccgctggc
     9061 cgagtggtgt ggtgatcgca ccggccgtcg agcgagcagg gcttgtccgg cgggcatctt
     9121 gccggccgca gagtatattc gcgtagtctc gagcgactcg gccaggctca gtggcggcag
     9181 gatcgtcgga atccgcttac tgagcatcgt tttgccggtt ccgggtggtc cgatcagcag
     9241 gatgttgtgt ccgccggcag cagccacggt gaccgcccgc ttgaccgact cctggccgcg
     9301 aacttcgctg aaatcctcct catatcgcga ggcgtcggcg aagacggcgt tgatgtcaat
     9361 caccgtgggc tccagcggga gctggccggt gagaaagccg acggcctcgg ccagtgtgcc
     9421 gatgccgatg acttcgatct cctcgacgac agccgcctcg ggggcgttct cgcggggcag
     9481 aatcaggcca ttaaaaccct tgtcgcgggc aaggatggcc atggacagtg cgcccttgac
     9541 cggccgtacg cgcccgtcga gggccaactc gccggcgatc aggtactggc ctgccggctc
     9601 gggcgtgcct tggccgttgg cgaagatcat gcccagtgcg atgggcaggt cgaacgccgg
     9661 cccttccttc ttcacgtctg ccggggccaa gttgacgacc gtcttgtatc gaggccagtc
     9721 ataaccggag ttgttcaagg ccgcccggac gcgttcgaga ctctctttca ccgccgcatc
     9781 gggcaagccg acgatggcgg ccttctcgaa cccgtgctcg gcgcagtcca cctcgatctc
     9841 gcacggcaga gcctcgatcc ccattaaagc aacgctgtgt aatctggaga gcatggaagg
     9901 attctacggg cgggttgcgg acaaggcaaa ctcacgggcg aatgatcctg ccggagcgtc
     9961 agggcactgt ccggtccacg acccgcagac caggtatgcc ggcgtagtcc tggacattgt
    10021 gtgtcatcag ggtcagatcg tggacaaggg cgacggcacc gttcaacaga tccatggcgg
    10081 gggcgggtct gcgcgcatcg agcaaaccgg cttgcacctg cccaaacctc tcggcgatcc
    10141 tcgcggtgac ttcaaggacc gttacatctg cgagcatttc ccgcaaacct cgaagtcgtg
    10201 ccggcggagc ctcggctcgc aatgcccaag cgtacaattc gccgagtgtg actgtcgaga
    10261 tgtgcaaccg accggcgtat tgcagaaagc ggttggtgac cacacctttc gctttgaggt
    10321 gagcagaaca aacgtccgta tcgacgagga agctcattca ggtcgttccc gtcgccccag
    10381 tttgcgctgc tgccgattcc attcgagata cctgtccaag ccttcggcgt catcagccca
    10441 agcccccgcc gatcggcgca agccctcgcc tggagacaga tcgctctcct tgtcagccgg
    10501 ttccatcgtg accttgacct gttgaccgtc cggcagcccc ggctcccgtt ccagttcgat
    10561 agtcttgccc cgcgcgatgc cgcgggtggt aacgctttcg ctgctcacgg gtcacctcca
    10621 gtcgcatcat accttgatcg gccgagcgac ggcatgaggt ttcgcccgga cgcgttcgag
    10681 gctttccttc acggccgcat cgggcaggcc gacccggcta cgccacaggt ctcgccccgt
    10741 gtaccggatg gggcgtaggc ttcgccgggg caagcgatgg cggccttctc gaacccgtgc
    10801 tcggcgcagt ccacctcgat ctcgcgcggc agagcctcga tggtcatgaa agcagcgctg
    10861 tgtaatttgg agagcatggg gggattctac ggggacatcg gcgataaggc agatgccttg
    10921 ggtcgcaacc gtaaagcggg accagattcc agacagcgtt tatgctttcc tgatccactg
    10981 tgcgggggtg atagcggaga cagtccgcgg gcttcgctgc agcatctcat catcccaggt
    11041 cacgagcgca gccttgagct ccgaagctgc cgcgacatac acggcatctg caccccgaag
    11101 acggtgatcg ccagccagtt gtgcggcctg ctttgccaac gcgagtgtca ggggaatcat
    11161 ttgcaggttt tcaattccct cgatcagacg tacgattcgt ccggcaagcg ctgagctttg
    11221 agtcatcctg gcaatcgcgc cggaacattc agccagtacc agtaccgggc aacagatcgt
    11281 ctctgcctca cgacgcaaca tcacgaggaa ctccacactc tcccgatagt gtgcctccga
    11341 tggccgtgaa gctgcgacga acacgctcgc atcaacgacc acactcatcg tcgggactct
    11401 cgaagggctt ccagggcaga cagtggactc ttccactctc ggtcaacctc acgggcgaat
    11461 gcctcccact cggattgcca atcggccggc tcagcctcga ctcgcactgg cgtcccctcg
    11521 gccagcggca tgccctcctc cagttcgatc ttgccgtgtt tcacgattcc cttgtacgtc
    11581 atgggcgacc tccatcggag attatagccc gatagaacgg tggtcgcata cactcaaccc
    11641 tgtgctcagc ggcaacatcc gcctggccga aaatagcggc cttctcgaac ccgtgctcgg
    11701 cgcagtccac ctcgatctcg cgcggcagag cctcgatggg cattaaagca atgctgtgaa
    11761 atttcaaggg cgtggctggg attctagaga caaagggcac gagcatcaaa tgaagaacta
    11821 ggaattgcct gtagggcagg accaaagatc gggtcaatcg gctgagggcg attgctgagc
    11881 gacgagggcg ggccggggct ttggacgggt tcgtttggcc cggcgggctt tcgggcgtcg
    11941 gtgttcggcg gcgagcggga tgggacagac cgagattcgt ccggcggata agtgacgggt
    12001 gatcgatact cgttgttcct ctgatgcggt agccggcggg attgtctgct gaataaagcg
    12061 gggctgcaag ctgggttcgt ttggcgcttt gcgtgaggcg tggggaaagg gccgaggtca
    12121 gagaccggct tgatcgatat aacctatttt cgaataatcg tttagcatta ttatgtatcg
    12181 ggatcttgaa gattttccgg atcatcatct gcctctgcga tatcagtctg gctgataggc
    12241 taaagctcct atagcggacc gcgctgagcg atcggccgtc ggtctccagg gggtcatctc
    12301 caatccgcac caagaggtac attggggcgg tcagccgcgc cagcggttgc gacaggtcgg
    12361 cgggttgctc atcggaaggc ggcacgatgg catcggttcg gacgctcctg ccatgagccg
    12421 gcgcgcagag tggccccgtc agtcctttgt gaacagaaca cgccatacgt tccggaaaag
    12481 tgagctggag cggaacgggg ggatggaaga acgaagggtg agggcgggag gatgagttaa
    12541 gacatgtata caacttgaag gtgtcttctt tggtggaaga cctgggaggt gtggcgggga
    12601 aggtggctat acaatggccc agaaggttcc ggtggggtaa ccgcgcaggg gcaccatgga
    12661 agagtcggcc gggcaccaaa ttctcgcccc ccctgcgaac gcatcttgga gtctgcccat
    12721 ggctgagtac aaggtcgtgc gccgctgccg atccgacagg atgatcgccg gtgtctgcgg
    12781 cggtctggcc gacttcttcg gcttgtcgtc cgccaacgtg cgaatccttt acgtcgccgt
    12841 cagcgtcctg tcggcggcct ttccgggaat catcgtttac ctcatcctct ggctgctcat
    12901 gcccctcgag gattaggcga tgcagagctt cgcgcgagtc gtccaggcct tcccctgcga
    12961 cagttcgggt gcgggtctgt gcctggttgc ctcggtctca gtcctcgggc tcctggcgaa
    13021 ttctccggtt cgcgataagc gagttgtcct gtgtcgctag tcgaggcaat cccggttcgg
    13081 gcggtcgcct gtgactcgcc aattcctcga ctacctgcgt cgggacaact acggaaccgc
    13141 acaggcgtgg caacacatca gcgcggccga ttcccacgag cgggaggagt ggggatgtgt
    13201 cggagatgac caacatctgt gtttggcggc gtggagttct tcaagagccc gagcgtcggc
    13261 gagatagtcg tcaatcgcac ggaggtcttc ggtggcattg tacttgtgca acacggcctc
    13321 tgaggcggag cgcctttttg cgggccgcaa cccaagtggt ctgcgatcca gaaacatgcg
    13381 cgcaaaatgc gcaccagtcg cgacggaaga gagtaacctg atcacagtcc aaggcccgag
    13441 ccggctcgaa acggctcagt cggcggcagc ggcaggccca caatggccgc cttttcgaag
    13501 ccatgttcgg cgcagttcac ctccacccgg caggtcaagg cctcaatccc cattaaagcg
    13561 acgctgctta acctggagcg catgggggca tcgtatgcct gcacacgaag ggttcaggct
    13621 gccacaggca tcgggtggcc ggccagtcga gcccggtctg tttctgcgcg ggggcttggc
    13681 agtcaggccc gcctgtcccg tcagattgac tctcacgagg accccgggca acatgggtct
    13741 cggtcctgca agaagcgtac gagcatcttg aaaccacact tgtcttcaat cttcgttgag
    13801 ggggtagtcc gaaaagagtc gcgggcctcg gtggattccg ttcagaaata ttttgcaaaa
    13861 aaagtcatcc agggtgtggt gagtcatagc atatccggca cgaatcggat aagatgatac
    13921 tgcatcaacc cgggccgcag cagtccggga cgggtcgagt gtaatcgggc gctattccag
    13981 atttgtggtt cctcgccgga atcgacccac acgtgaggct acgagcctgc tctgagagtc
    14041 agccgcgtcg gctgacaatt tcgcgttgtg tgtatcgtca acaagtcgac gtgcccacag
    14101 aggtgaggga cccgcatagg gagaggagag atcgtcgtga ttcacctatc tgtttccaca
    14161 cgcaccacca tgtggggtct tgcagcaatc ctctgtttcc tgacgttggc gggtgcagct
    14221 caggcggcgc ctctgctgtg ctggtccatg cacgacggca tacggacctc gaaccttgac
    14281 gggaccaaca tcaacacgtg gctgtcgaag cccaattatc ccatcttcgc cgaggtccac
    14341 gacagtcctg ccgtgggaaa ggtctactgg tttgaatcgg gcggttacat caaacgcgct
    14401 gactacgacg gcggcaacgc tgagctcgtg attaccgaca ccaagatgta cacgaccgcg
    14461 ggggactttg ccatcaaccg cctcaacggc gacgtgtatt gggcgaatgt caacggcgag
    14521 ctgtggcggg ccggtccgga tgggtcgaac aagcagctcg tgcgctccgg cctggcttcg
    14581 tccggggcga tcgagattga ccctatccat cagaagctgt tctggcttca gtatagcgtc
    14641 gggaccgaca tctacatggg ggacctgaac ggcggcagcg ccacgtacct gacctccgta
    14701 agttctctgt ggaaggttac cgacctggcc gtcgacacgc agtccagccg cctttactgg
    14761 agcgagaccg gcgttacggt gaacgagcgg gtgcgtcgtc tcggacttgg accgggcacg
    14821 ccctccgtga tcgtcgatgc agaacttggg tacctgaacg atgtgaccct cgatctggac
    14881 tccggcaggt tgttgttggt ggaccgctcg ggcgaccgca tcctgcagtg cggccttgac
    14941 ggcagcggtc tcaccacgct gatcagcggt gaagatgagc cccagcacct caccatcctg
    15001 ccggttcctg aaccggcgac aatggttctg cttggtcttg ccggggcggc tgtcctgtac
    15061 catcgtcgca ggttctaaac gacgaagcat tgacggcggg agcccgcggc atcggcaagc
    15121 gtgagaaccg aaagagaccg gcccctgcgc cgggagcaga tccgtacatt gatcggctct
    15181 ggtcgcgatt cccacacggc caacccctgc gccgggaaca gatccgaaaa ccatcttgtt
    15241 gccgggtcgc ggatgccctt cgttgttctc tggtccacgt tgcccgcaac cacgtgggtt
    15301 ggcaacacga tgctgcacgt ggatggcaga atcgtatctc gcacgcaacg atctgcggcc
    15361 cgggcatcgc ggagtcaagc ctgctctcgg gcgggcccga gccaggaccg ttctctgact
    15421 cttcttttgg cccaggtttg gcaggaccgg caagagcgac ggacgaacga ccgcacgttt
    15481 ggcggaaagg cagactcttt cggacattcg tcgttcacat ctggcatccc gatggatcag
    15541 gttattcccg ggcctggctc ttggtttccg cgtggaggcg ccggcgctcg gcggccttgg
    15601 caaaggggta gtaaaccaac atcgccagtc cgatcgaaac gactccccag actgccgcct
    15661 tccagtcgcc gccggaaacc aggtagtgcc cgatgaccgg cggggttgtc cacggcacgg
    15721 cgacaaacgg cttttgaatc actccgcagc tcattagcag gtaggaaccg gtggtgagga
    15781 tcaacgcgtt caagatgtag ggaatcatca agatcggatt caggacgaca gggaagccga
    15841 agaagatggg ttcgttgatg ccgaagatct gggtgggcag tgagagacgg ctgatcttgc
    15901 gaaaggcggg ctccttcgat cgcagcatga ccaaggcaag cccaatggtg gcacccgtcc
    15961 cgccgacgtt gacaaaactg gtgaagaagc cgagcgcggt cacatagggc agcggctgcc
    16021 ctcgcgaagc ggcctccaca tttgctgcca ggtattgcag gaagacgggc gcgacagcgg
    16081 cgtccatggt gttgtcgcca ttgatgccca cggaccacag gagggtgacg agaaacgcgt
    16141 acaccaggat gccgggcagc gtgttcaggg caaagaccag cggggtcaac acggtttgca
    16201 gcaggtggtt gatgtccaac cctgccacga accggatcaa ccagaaaacc actaccagaa
    16261 agaacatcgg gctcagcgac aggaacgatt catagacgac cggcggcaca ttccggggca
    16321 gcttgacgac gaggttcgag tccgtgaacc acttctggac gcgcacggtg atcagggcga
    16381 tgagaatggc cgtaaacagg cctttcgagc cgaggccgtc catggaaaag ccctggtcat
    16441 ccggcttgag ttgaaccatc aggaaaacca gcgcggccat ggcggcgctc acaatggcct
    16501 cctgattcag ccgcttcccg taatcgtacg caacggcaaa gcacgcgaag acggcgagca
    16561 gaccaaaagt ggcggtgacg gggatctgca gcaacggcag gcagggcgcg acccgatcgt
    16621 tccagccctt caccgggaga aacgacacga tcatgaacaa gccgccgatg atcgtcagcg
    16681 gcacgacggt catcataccg gcgcggattg cggacaggca cgtgttctcg ctcagcgtgg
    16741 tgagcgacgg cacgatgtat cggttcaata ggccgccggc ggagttcaaa gtctctttcc
    16801 caagtaaatg cagccatcct tgtcccctcc aacccgaacc aactcgtgga agccgagctt
    16861 ctcatagaac cccaaagccg gggtattgag cgtgctcacc cccagatgcg cgccgggtga
    16921 accgcgctcg cggagtcggt tcatcacctg ctccatcatg cgtcgtccga aaccaagccc
    16981 ccgcgcccgt tgaagcaggt cgatgtgcag gtgcgagggg aaaaccgcat atggctccgg
    17041 acagaaatag tcgggatggt gatagcagta gtgaacttgc tgagcccgtg tccatcgacc
    17101 gggatcgcct tgcggctcgg ggaaacgggc gcacagttgc ggccgccatt cggcctcata
    17161 gcgcgcgtaa aatgagcgag agtcgaaggc cgccaaagca tagccgcaaa taccttgctc
    17221 atcctcgagt atcaggctca gctccggctc aaaagcaagg taggggccaa cgaagattct
    17281 tcccaatgca tccggatcgt cgcggtaaag cggctcacca tccttgccgt tgtctccggt
    17341 cttcaggcag acatagtagg cgccggcctc gtcgccggga agggcggaac gaatcgtgaa
    17401 tctgttcatg ctgccgatgt gcatacagga ttggagtgcc tcgagtcaat acctaccggc
    17461 ggcgcgcaag agccatcagg gttttgagtt acgaggcgcc gccatcgcgc cgctatgccg
    17521 ccgcgaaagg tgccccggcg aggggaaccg gggcatcccg gggtcttccc ggttccggac
    17581 gcggaggcaa ccgcgcacga agctcgtggg atccgcgctc acggagcaat ttgcggatag
    17641 ccgacccttg acccgcggcg cggcctggcc gcgagagatc gcgaagggac cgccgcgtgt
    17701 caagggctga tcgccgaccg ctcgctccag ccaaacccga cgtcgagcgt gataacccaa
    17761 ggcggaccgc ctttttgcgg gccgcaaccc aagcgcactg ccatccagaa acatgcgcgc
    17821 aagccgcacc gatgtccagc tgaaaggttc tatcagccag gtgcgaactc ttttcggcgg
    17881 cgtccggctg gaagaacgta tgaaaacggc aaggttgggt cgaagtcacc gaagacgggg
    17941 ttgccagcct cgattgcgcc ctcttggcag atctcggtgg ccacggggac ctcgatactt
    18001 ctccaccgac cagcgccgtc ggttttcacg tatattcttt ctactcgacc gatcccaggc
    18061 cgaccgtcag gtcgaaggga actcggacgg tcttcatatc cgtccaatag gtgacctcaa
    18121 cggtcgcggt ctcgacctgc cgggccagcc tgtaactctt gttgagcttg accatgccgg
    18181 cgagtctcat gctcgccgag ctcgaagcgg aagacttgat gggcgtgccc ttgtcgtcaa
    18241 gaaagcggac gcctgcgagg ctgtcacccg ctttgcccat ctcgaatgtg acttcgaggg
    18301 gctcatcgcc ccattgcggc ttaccggtct gtttgatggt gatctcaatg ggcccgacgc
    18361 tgaacgtggt gcctttcttc aaggccacgg ctttctccac ggccgtcttc ttctgcgtgc
    18421 cggtcttgaa ggcaaggctc ccctttgcgc gcaactctcg ggcgcccttg ccgggcaggc
    18481 gcttggcgga caactcgatg atggcggcct ttccgtcttt gctgacctgt gggaacgaac
    18541 cgaagccctc gtcgaatgat tcctttttgt ccagaaggct gtcgcccttg tcgttcgtga
    18601 aggccgacaa cacgctttcc tgttcgtcaa aaccgatcaa ctcgcccgac ggttgcctga
    18661 cgagaatcgc gagcgtcgtg ccctgcggct gattgaaggg ctgctgttcc gattccttgt
    18721 ctttgaaacc tgttccgacg acgcggatac cacagatgtg gatcgtgggc tttgccgcct
    18781 cgtcggccgc ccggaccggc gttatgctga gaatcgcaat gaccgacatg gtccccgcga
    18841 ccatgatgct cgcgcccgtc accaagagac cgcttctgat tctcgaggac atcgatggac
    18901 ctccgttcaa ggacgaaaga aacgttcagg gagtcgtcgg gggatagcat acctgtttct
    18961 tgcgttgggt tccaaaatgg tcttctgccg gctgcgggcc tcggatagcg agggtggttt
    19021 tgcctggcta atcgctcgca tcacccggcg gggcgatcac tgggagggga gtggcgggag
    19081 ccaaagagcg gtcttccctg cggccgggga acggctatga tagtcccatg cgttgggtcg
    19141 ttgatttgtg ctatcttctg gcggcggcgg cgtttgcgcc gattgtagcc tatcgacgtt
    19201 ggctgacggg caagtatcgg cgcgactggg accagcggcg gggcctgttg ccgcagttac
    19261 cgccgggt
//
LOCUS       DAOTPW010000100        19222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2908_length_19222_cov_4.468983, whole genome shotgun sequence.
ACCESSION   DAOTPW010000100 DAOTPW010000000
VERSION     DAOTPW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19222
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_19222_cov_4.468983"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            925..1839
                     /locus_tag="P5316_10685"
     CDS             925..1839
                     /locus_tag="P5316_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR85463.1"
                     /translation="MGQVLNRRTFLGASLAFAGGLATSASAAHGETESAAGGQSGGQR
                     GRQTRLGLVTYNLAADWDLDALIRRCREVGLSAVEFRTTHKHGVEPSLSKEQRKEVRK
                     KLEDSGLSAWSLGSVCEYHSPDPVVLARNMEDSRRFVELAADIGAKGVKVRPNGLPKE
                     VSEDKTLEQIGKSLHAVGEMAAAAGVEIWCEMHGSGTAEPSRMRRIMDVADHPKVGVT
                     WNSNGVDVKNGSVKESFELMKGKIYCVHINDLVGGYPYRELFSLLNQAGYDRSALIEC
                     PALKSGNADDTVRFLKYYKALWEEWSKT"
     gene            complement(2014..3150)
                     /locus_tag="P5316_10690"
     CDS             complement(2014..3150)
                     /locus_tag="P5316_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRR85464.1"
                     /translation="MLIHLLAIGAAMAVAQATEPPLRFERLRIGDVTFEAAAAFDVNN
                     DGRLDIVSGGCWFEGPEFSRKHKIADVMRVEDYYDDFSCYPMDVNGDGYQDIITGGWW
                     GGTLRWRENPRGRPIEWVSHDIDKCGNIETTRFWDVDGDGQAEVVPNAGGNIVVYRLI
                     RGPDGKGTGRFEKHVIKEGGCGHGFGFGDVNGDGRGDFIIPNGWIEAPNQPYRQAWTL
                     HEEFNLGTTSCPVLVYDVNKDGRADLIEGQAHGYGLNWWEQKTGADGKRTWIKHPIDP
                     DRSQYHDLVLADIDNDGQVELITGKRYRAHLGRDPGAMDPVGTYYFEIDGGRFRRVTL
                     DYGPASKASGVGIYLWVADVDGNGWKDIVAPGKEGLYLFRNLGR"
     gene            complement(3160..5334)
                     /locus_tag="P5316_10695"
     CDS             complement(3160..5334)
                     /locus_tag="P5316_10695"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR85465.1"
                     /translation="MINRLSRLTCVAAISTVTLAGGCSGVAGPSGVVVPQKSSVEQAF
                     AALPAMASQSDALIAQLAGDDEKARSEARQLLPREGIEVVPKLVPLVGHEKAAVWSAA
                     FNVLADFANQVSIPGREADRVRVAACIMTLVAPDRPEEVKIRGLRLLPLVVPEGFDVG
                     PMAALLYDASLQEKARAAFEEMGTPEASTALRDYLPKAKPDFQMAILNSFARLQRMAN
                     VQAALAMTKSENAAVRAAALRAIAWTGDPSLLNVARAVVAVADASTHAEAMDGMLRLV
                     GAIADRGGNWQLAVNTYLDILKTGQGMDRDGAIAGLGQIGDGTCVRPILDAIQDAEPH
                     TWRAGIGALTSMQGVDVTRAIVESYPRVPRNTQLALVSVLGDKRHAPAAPILTQAAQS
                     EDPAFRMAGLKALAAAGLPEGIDLLTTAVRAGSDEEKAVARQGLLAVADSLRAAGRKN
                     EAGTAYLASFDNAAATDHDTRQRAIEGIAACPIADAAEAVKAAATDKDLRPSAMSALL
                     SVGSALTAAGKKDAALELYQTLTQMSPPLEIMREVAKGMAAAGAKVDLQGLLGTITNW
                     WVVGPFELGEENKGWNMRYVDEPNVNLSGRYMSGKRRVQWKAVVSEDPNGKIDLRKTV
                     ADGEQCIAYAYTEITVEKPTDAVLLVGVDDSEKIWVNDEQVFELWQARAMVVDNDRVP
                     VKLKAGTNRILMKIWQNTMGWEFCMRITRPDGSPVSFAQKTE"
     gene            complement(5370..6665)
                     /locus_tag="P5316_10700"
     CDS             complement(5370..6665)
                     /locus_tag="P5316_10700"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85466.1"
                     /translation="MRIGRDISRRQLLRISGQGIVFAGLGPLLRTRPCHGDISLNEKV
                     TIAIIGCGGMGTRHIEALSVNPQCTIAAVCDCFKPRYENAIGVVDKLSGKKPDGYQDF
                     RRVLDRKDIDAIWAPTPDHWHPLMTILGCQAGKDVYVEKPVCPTVAEGRAMVQAARRY
                     GRVVQVGTQQRSMTIFQDAMKLIHSGRLGQITSATCWVGVNGWQVGETPRPVPEGLDW
                     DMWLGPAPKVPYSPERHYGFMGWHDYCRGGQLTNWGVHLMDIVHWGIGEDRPLSVQAL
                     GGSYRGGAGADNYENIEALFEYKGCTVTWEQRHSNQYNGHDYGIKFQGTKGSLTIDRN
                     TFEVWPGRLGIPQYVGEPERSWAHPPHHNNFFDCIRSRKLPAADIEQGFRSTIPVLLA
                     GIALKVGRKLLWDGENERFIRDAEADRHLSRAYRAPWHL"
     gene            7698..8561
                     /locus_tag="P5316_10705"
     CDS             7698..8561
                     /locus_tag="P5316_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85467.1"
                     /translation="MRYLVALFVFGVMAAGASAAVVVGGTETFTSGVNGGADWGVGGA
                     SAYDGWQVDLFKGYDSRPGNNDHKEYFGSTGQILTDWPDGRKSKGQPDPGSHSGDPGT
                     LVVQHNAFWNGADPNKGETDPPSTCTQLKYISFNSVAGTTYELTGWVNTVYIQHGTAW
                     DGMHPRIVDHGTPPMPAQFPDIQIGLKNGLADANDLDVTYTQVTNLIDNMAGGAPNPN
                     AWVQFSVSAVGDGGPMTILLKVAAPDISSGAVHDTRTWDVDVRFDDITLTPEPTALVL
                     LSLGLFLVRRR"
     gene            complement(8670..10373)
                     /locus_tag="P5316_10710"
     CDS             complement(8670..10373)
                     /locus_tag="P5316_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRR85468.1"
                     /translation="MRSTPSLTDRVMYVIARHHAEAVYRRFMAATRKATEVQKQVLAA
                     HVRRNADSDFGRDHLFDRIGSVDAFRRQVPILRYEDHRPYIERVCNGRLQAMFGGRQR
                     VRMFALTSGTTDRPKYIPVTDEFLRTFRRGWNVFGIKALLDHPQAFLRGIVQVTSRMD
                     ESRTPAGIPCGAITGLMAATQKRLVRKYYVAPQCTAQITEPVAKYYAIMRLAIAQDVA
                     FMITANPATQLKLARTADEHRERLIRDVHDGTLSFELFVPAQVFASLKDRLRPDHERA
                     RELEGIVERYGALLPKHYWNLAFIANWTGGTMGLYLQDFPKYFGDVPVRDIGLLASEG
                     RISIPMEDYTPAGILDTQSHFFEFVPPDRIEETNPKTFLCHELDVGQEYFVLMTTASG
                     LYRYHIGDLVRVVGYEGQAPLIEFLSKGAHICSLAGEKLTEHQVMQAVSQAAGRTGIG
                     VTGFVLAPRWGDPPYYMLHIETRGAGDEVLEELTAEVDVRLSTLNIEYAERRRSLRLG
                     AIRGNALPDGFLARLDHEQAESRRRGNEQFKHRYLYSRPGEDAAFPSRQALGSTGRPT
                     E"
     gene            complement(10445..11269)
                     /locus_tag="P5316_10715"
     CDS             complement(10445..11269)
                     /locus_tag="P5316_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR85469.1"
                     /translation="MARIQIGINMEFVRHHDMSFVAGVEKAAEIGYEFIEPMVHWGRE
                     LMSEAGYFHTVSMFEDPLDIRAICEKYKVRCSGLSAHSPLCKPDISVNYLRSAIRFAA
                     ELAAPIVNTDEGPKPDFTDEATDHVLMTYTLTLATRAAEKRGILIGLEQHQQYSKTPA
                     GLDRIYGLVKSKSLGINFDTGNAYLAGADPYEWLERCRDRVIHMHAKDISLKQSQDER
                     GKVTGTPVGCACGEGVIDWKRVIDILKPVQRTICFSVECGTIDQAAASLDHLKSLI"
     gene            11595..12455
                     /locus_tag="P5316_10720"
     CDS             11595..12455
                     /locus_tag="P5316_10720"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRR85470.1"
                     /translation="MIATPKGTHDRHRLQCMEIWGGNRAVEDTVSVFGIDAWVLSLPH
                     AEGQQGGDIHYVSMCGSGRIARFIVADVAGHGNAAADLAVVLRNLMKKHINRLDQTKF
                     ARDLNREFGLVANDGSFATAVLATYFAPTGHLILCNAGHPPPLWYRSRDRRWRPLVPT
                     MPEQVSRVRNLPLGVIEPTDYQQFAVSLSEDDLVLLYTDSLIEAANPAGARLGLDELL
                     GILERLDGSRPEGLNHEIVAGVRKWASPVRLDDDTTLLLLRQNGERPSWPSFSTTVKV
                     LGKLIGLVDY"
     gene            complement(13523..14299)
                     /gene="thyX"
                     /locus_tag="P5316_10725"
     CDS             complement(13523..14299)
                     /gene="thyX"
                     /locus_tag="P5316_10725"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRR85471.1"
                     /translation="MRIIREPKVYLLGRQTIDPEELSRFLADHEVANWTTDTDIAGQK
                     LCEIAGRVCYMSFAKPRPGGNKAYLGHLLEVGHGSVLEHAVFNLLITGVSRSFTHELI
                     RHRAGFGYSQLSQRYVDESTADFVEPDCIARDPGLHAVWLRAIEKSLEAYRELVNALM
                     NAPWQVQDKTLQRKIARQAARSVLPNATETKIFVTANARALRHFIEMRCSESAEVEIR
                     KVAAEVLRVLQKEAPNLFGDYELHPLPDGTFEARTRHRKV"
     gene            14624..15493
                     /locus_tag="P5316_10730"
     CDS             14624..15493
                     /locus_tag="P5316_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRR85472.1"
                     /translation="MMVANVQDYVGRVTLADGAWGTELDKLGCPPGYCREEWNISKPE
                     LVEKVAASYVAAGSEIILTNTFGGNRIVLASHGFEARMREFNEAGACISRRAAGHRAK
                     VFGSMGPSGKMLLIGEVDEKTLYDVFREQAEALAAGGADGLVVETMADLTEAMTAVRA
                     AKTTGLPVVGSMTFDSGRDKSCTLMGVTPEQAVQGLTEAGADVIGCNCGIGIDNYIKV
                     AGRLRAATSKPIWVKANAGLPEIDGSRVVYRMTPKEFAQKAREVIEAGANIIGGCCGT
                     TPEFINVLAAAVR"
     gene            15637..16752
                     /gene="rho"
                     /locus_tag="P5316_10735"
     CDS             15637..16752
                     /gene="rho"
                     /locus_tag="P5316_10735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRR85473.1"
                     /translation="MPESIVDGTLELQENGGGFLRSPRRNYAVHSTDAFVPDSIIRRW
                     HLRGGETVTGLGVVNGHGGRSRLVLSAVQSVNGLEPEAYGELPDFAELTVIDPTSRLW
                     FETPGGSLSMRVIDLMTPIGLGQRGLIAAPPRTGKTVLLQQMAAGLAANHPEVYMMVL
                     LIDERPEEVTEMRRTVRGEVVASSNDQNVASHIRIARLMIEKAKRMVETGRNVFILLD
                     SLTRLGRAYNAGIRSSGRTMSGGIDIRALAEPKAIFGSARNIEHGGSLTIMASALIET
                     GSRMDEVIFNEFKGTGNMEIMLSRDLANLRLWPAIDLNQSGTRKEELLLGKEVTEKTY
                     RIRRQICNQPPNKAMEMLLDSLRKFPSNQAFLDSMPL"
     gene            16925..17284
                     /locus_tag="P5316_10740"
     CDS             16925..17284
                     /locus_tag="P5316_10740"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR85474.1"
                     /translation="MVTIATGEELLAELRRHFRLVEIGNDWPGSCNRRRHTRYELESG
                     SVPVEVCSRFGLTARGLLADIVVGGARVITDYVPAIGEILSLSFNVNNNFYVLDAEVR
                     HVGVEGSIRYVGVRFVE"
     gene            17342..18412
                     /gene="cheB"
                     /locus_tag="P5316_10745"
     CDS             17342..18412
                     /gene="cheB"
                     /locus_tag="P5316_10745"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HRR85475.1"
                     /translation="MAHSNIRLLIIDDSTFVRTILRSALSGQRNIEVVGFASGCEEVI
                     EKIDHLQPDVVTVDVDMVDRQGLEILNLVQSRCSARIVVIASLNRESASMAMEALERG
                     AFDFVVKPRKTGVDGIPRFRELLCEKILAAVRAAKSRGSRGPLADVPEELIASGIKGR
                     WVVGIGIGTGGPQTLARMLPSFPSDFPPVLVAQHMPPYFSTVLAERLAATCRMVVREA
                     VDGQPVKTGEILIAPGGLHMQVARRGEELIVVLEGGPRVSGHRPAADLMFESIAGACG
                     AKAVGVLLTGAGRDGVAGLAKLREHGAWTVAQDRATSYVFGMAGAAIRAGVVDQVLPL
                     PKIPRAIVSLMAQGCRETVTAS"
     gene            18793..>19222
                     /locus_tag="P5316_10750"
     CDS             18793..>19222
                     /locus_tag="P5316_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85476.1"
                     /translation="MNTVIPPGTQGGGRAVFMGQAVRPIQGGTAGGLGQNWYRPQGAT
                     ALQRLYREAETKGPLGLERRPSRWDSRSRYSGISALSGSSEVEVGTSVGAGTPDRPRV
                     TDSRPAEFEISRPTGALSQAGLAARRAAYLDAGWALFKEGK"
BASE COUNT         3590 a         5620 c         6029 g         3983 t
ORIGIN      
        1 agactcgcac cccttgtgac tcttggccgc agaccgagta tctgcaccgc ttagacccgc
       61 cccttcgcat gcttggcaaa gcgttcctta caagttacgg ggcaaatcac ggcaaatcac
      121 atagtattgt accttccagg tcggatgttc gtcaatgaaa gatttgagaa gagagttctt
      181 tttcatcagt ccgattcatg atcctccgtt ttcagtcgga agacggagta tctgatcgag
      241 ttcgggcgtt tgctttttcg tcccataact cacgatgggt gcatgtttaa gaagcgaggg
      301 cgacgtactt cattggcagc taggggctgg tccgaactgc cgaacgcgac ggatcgccgt
      361 ctctctgtcg cacccgggcg ggatgtgcct ctctgtgctg cagcaggcag tcgttcccag
      421 cttgcttgaa ccaggggcaa agtgcaccaa agggtcgggt ttgcggtccg tggacattag
      481 atgcatccct gagagctctc aggcggaatg gactgcttga tcgtccgtct cggtctggac
      541 gagaacgggc catatcacga agtcgcacgc ggcaaggtgg tcgccctctt tcggtccggt
      601 gcgtgacagc gagcctccag ccgcttcctt ccggtcgggg ttccgaacga gtcacttgta
      661 agagccgccc cgtttcccag cgtatcttgt cggcgacccg ctcttgggcg tcgcggtcgg
      721 caagcttgcg tttccgggac tttcgggcaa cgattcggat ttgcatacgg ccggattgcg
      781 gcttgcggga tggggcgaac cccggatggg catgcgaaat gcgtggctca ggcgaaatcg
      841 aatgcacggt ggctgggcgt cattgaccag gccgccgccg atggggcctg tggcccgtgg
      901 taatgattat tgaggaggtt taccgtgggg caagtattga acaggcgtac gttcctgggg
      961 gcttcgttgg cctttgccgg cgggctggcg acgagtgcga gtgctgctca cggcgaaacg
     1021 gagagcgctg ccggcgggca gtcaggcggg caacgcggca ggcaaacgag actggggctg
     1081 gtcacgtaca acctggcggc ggactgggac ctcgatgccc tcattcgccg gtgccgcgag
     1141 gtcggcttgt cggcagtgga gttccggacg acgcacaagc acggggtcga gccgtcgcta
     1201 tccaaggagc agcgcaagga ggtgcggaag aagctggagg acagcgggct gagcgcatgg
     1261 agtctgggtt cggtttgtga gtatcactcg cccgatcccg tggtcctggc caggaacatg
     1321 gaggacagca ggcggtttgt ggaactggcc gccgacatag gtgccaaagg tgtcaaggtg
     1381 cggccgaacg gcctgccgaa ggaggtcagc gaagacaaga ctttggagca gatcggcaag
     1441 tcgcttcacg ccgtaggcga gatggccgcg gccgccgggg tagagatctg gtgtgaaatg
     1501 cacgggagcg gcacggccga accgtcgcgc atgcgcagaa tcatggacgt cgctgaccat
     1561 ccgaaggtgg gcgtgacatg gaactccaac ggcgtggacg tcaagaacgg ctcggtgaaa
     1621 gaatcgttcg agctgatgaa gggcaagatc tattgcgtgc acatcaacga tctggtgggc
     1681 ggataccctt accgcgagct gttttcgctg ctcaaccagg ccggttatga ccgctctgcc
     1741 ctgatcgagt gcccggctct caagtccggc aacgcggacg ataccgtacg ctttctcaag
     1801 tactacaaag cattgtggga agagtggtcg aaaacgtagg acggcggccg aaaggtccgc
     1861 ggctgttgcg gcatggggat gccgcggccg aaacaccggc acttggttgt tggccgaaag
     1921 ccgacggcca cctgctcctg ccgcagtggc tgcactaccc tcggtccggg accggcgagg
     1981 atgccttgaa ggcattcgcg tttgttgttt tcctcatctt cccaggttcc tgaacaggta
     2041 gagaccttct ttgccggggg cgacgatgtc cttccatccg ttgccgtcca cgtcggccac
     2101 ccacaggtag atgccgacgc cgctggcctt cgaagccgga ccatagtcca gtgttacccg
     2161 gcggaacctg ccgccgtcga tctcgaagta gtacgtgccc acggggtcca tcgctccggg
     2221 atcgcgtccg agatgcgccc ggtaccgctt gccggtgatc aactccacct gcccgtcgtt
     2281 atcgatgtcc gccaggacca ggtcatggta ctgggaacgg tccgggtcaa ttgggtgttt
     2341 gatccatgtc ctcttgccat ccgcaccagt cttctgttcc caccagttca gcccgtagcc
     2401 gtgtgcctgt ccctcgatca gatcagccct gccgtctttg ttgacgtcgt acaccaatac
     2461 cgggcaactc gtggtgccca ggttgaactc ttcgtgaagc gtccatgcct gcctgtaggg
     2521 ctggttggga gcctcgatcc atccgttggg aatgatgaaa tcgcctcggc cgtctccgtt
     2581 gacgtcgcca aaaccgaagc cgtgtccgca gccgccttcc ttgatcacgt gcttttcgaa
     2641 tctgccggtg cccttgccgt cgggccctcg gatgagtctg tagacgacga tgttcccgcc
     2701 ggcgttcggg acgacctccg cctgcccgtc accgtccacg tcccagaacc gcgtggtctc
     2761 gatgttgccg cacttgtcga tgtcgtggct gacccactcg atcggccggc ccctggggtt
     2821 ttcccgccag cggagtgtcc caccccacca gccgccggtg atgatgtcct gataaccgtc
     2881 cccgttaacg tccatcggat agcaggagaa atcgtcgtaa tagtcctcca cccgcatgac
     2941 gtcggcgatc ttatgttttc gcgagaattc cggcccctcg aaccagcacc cgccggagac
     3001 aatatcgagg cgaccgtcgt tattcacgtc gaacgcggcc gcagcctcaa aggttacgtc
     3061 accgatgcgc agccgctcaa aacgcagcgg tggttcggtt gcctgagcaa cggccattgc
     3121 cgcgccgatg gccagcagat gaatcagcat gttgcttcct cactcggtct tctgggcaaa
     3181 cgaaaccggc gaaccgtccg gtctggtgat tcgcatgcag aactcccagc ccatggtgtt
     3241 ctgccagatc ttcatcagaa tcctgttggt cccggccttg agtttgaccg gtacccggtc
     3301 attatcgacg accatcgccc gtgcttgcca caattcaaac acctgctcgt cgttaaccca
     3361 aatcttctcg ctgtcatcga cgcccaccaa cagaacggcg tccgtcggct tttcgaccgt
     3421 gatctccgta tatgcgtagg cgatgcactg ctcgccgtcg gcgaccgttt ttctcagatc
     3481 gatttttccg ttgggatcct cggataccac cgccttccac tgaacccgtc gcttgccgga
     3541 catgtagcgg ccggacaagt tgacgtttgg ctcgtcgacg tacctcatat tccaaccttt
     3601 gttctcctcg cccaactcaa acggcccaac cacccaccag ttcgtaatcg tgcccagcag
     3661 accttgcaga tcgaccttcg cgccggctgc ggccattccc ttggcgacct cgcgcatgat
     3721 ttcgagtggc ggactcatct gcgtcaacgt ctgatagagc tcaagagccg cgtccttctt
     3781 gccggcggct gtcaaagccg agccgacgct cagcagcgca ctcatcgcgg acggtcgcag
     3841 gtctttgtcc gtcgcggcgg cctttaccgc ttcggcggca tctgcgatcg gacaggcggc
     3901 aatgccttca atcgcccgct gacgggtatc gtggtcggtc gcagccgcgt tgtcgaaaga
     3961 tgccagatag gccgtcccgg cctcgttctt cctgccggcg gccctcagcg aatccgccac
     4021 tgcgagcaga ccctgccggg cgaccgcttt ctcctcgtca ctgccggccc ggacggccgt
     4081 cgtcagcaga tcaatgccct cgggtaaccc cgctgccgcc aaggccttga gccccgccat
     4141 gcgaaacgcc ggatcctccg actgtgctgc ctgcgtgaga atcggtgcgg ccggcgcgtg
     4201 tctcttgtcc cccagcacgg aaaccagagc tagctgagtg ttgcgaggaa cgcgcggata
     4261 ggactcaacg attgctctgg tgacgtctac accctgcatc gaggtcaggg ccccgatgcc
     4321 ggcacgccag gtatgtggct ccgcgtcctg aatggcatcg agaatcggcc tgacgcaagt
     4381 gccgtcgccg atctgtccca atccggctat agcgccgtcg cgatccattc cctggccggt
     4441 cttgagaatg tcaaggtatg tgttcaccgc cagctgccag ttccctcctc tgtcggcaat
     4501 ggctccaaca agccgcagca ttccgtccat agcctccgca tgtgtcgagg cgtcagcaac
     4561 agccacaacc gccctggcga cgttcagtaa actgggatca ccggtccatg caatggctcg
     4621 cagagcggcg gcccgcacgg ccgcgttctc gctctttgtc atggccaggg ccgcctgcac
     4681 gttggccatg cgctgcaggc gggcgaacga gttgaggatc gccatctgga aatccggttt
     4741 cgccttggga aggtagtctc ggagggcggt gctcgcctcg ggcgtgccca tctcctcgaa
     4801 tgccgcgcgt gccttttcct gaagactcgc gtcgtatagg agagcggcca tggggccgac
     4861 atcaaaccct tcaggaacga ccagaggcag gagtctgagt cctcggatct ttacctcctc
     4921 cggccgatcg ggggccacga gggtcatgat gcaagcagcc actctgactc ggtcggcctc
     4981 gcgacccgga atactcacct ggttggcgaa gtcggccagc acgttgaaag ctgcgctcca
     5041 gaccgcggcc ttctcatgcc cgaccagcgg caccagcttg ggtaccactt cgatgccttc
     5101 gcggggcagc aattgtcggg cttcggaacg agccttctca tcatcgcctg ccaattgggc
     5161 gatcagagca tcgctctggg aagccatggc gggcagggct gcaaaggcct gctcgaccga
     5221 gcttttttgc ggaacgacca cgccggatgg cccggccacg ccgctgcaac cgcccgccag
     5281 cgtaaccgtc gaaatcgcgg cgacacacgt gagtcgagag agtctattga tcacaggtat
     5341 gctcctgttc tggttccgat ccccggattc tacaggtgcc acggagcccg gtaagctcgt
     5401 gacagatgac ggtcggcctc cgcgtcgcgg atgaaccgct cgttctcgcc gtcccacaga
     5461 agcttgcggc ccaccttcaa cgcgatgccc gcaagcagca cgggtatcgt ggaacggaag
     5521 ccctgttcga tgtccgcggc cggcagcttg cggcttcgga tgcagtcaaa aaagttgttg
     5581 tgatgcggcg ggtgagccca actccgctcg ggttcgccga catactgtgg tatacccagt
     5641 cgtcccggcc agacctcgaa cgtgttccgg tcgatggtca gcgaaccttt ggttccctgg
     5701 aacttgatgc cgtagtcgtg tccgttgtac tggtttgagt ggcgctgttc ccaggttacg
     5761 gtacagccct tgtactcgaa caaagcctca atgttctcat agttgtccgc cccggcgccg
     5821 ccccgatagc tgcctccgag ggcctgaaca ctcaaggggc ggtcctcgcc aatgccccaa
     5881 tgcacgatgt ccatcagatg aacgccccag ttggtgagct ggccgcctcg gcagtagtcg
     5941 tgccaaccca tgaatccgta gtgccgctct ggggagtaag gaaccttggg ggccggtccg
     6001 agccacatgt cccagtccag tccttctggc accggtctgg gggtctcacc aacctgccag
     6061 ccgttgacgc cgacccagca agtggccgaa gtgatctgcc ccagccgccc gctgtggatg
     6121 agtttcatgg cgtcctggaa gatggtcatt gaccgttgtt gtgtaccgac ttgcactacc
     6181 cggccatagc ggcgcgcggc ctgaaccatg gcccgcccct cggcaacggt ggggcacaca
     6241 ggcttctcga cgtacacatc cttgccggct tggcagccca ggatggtcat cagggggtgc
     6301 cagtggtcgg gtgttggcgc ccagatggcg tcgatgtcct tgcgatccag cacccgtcgg
     6361 aaatcctgat aaccgtcagg cttcttgccc gacagcttat cgacgacgcc gatggcgttt
     6421 tcgtatcgcg gcttgaagca atcgcacacc gcggcaatcg tgcattgcgg gttaaccgag
     6481 agggcctcga tgtgccgcgt tcccatgccg ccgcatccga tgatcgcgat agtcaccttc
     6541 tcgttgagcg agatatcacc atggcagggg cgggtacgaa gcagggggcc caggcctgcg
     6601 aagacgattc cttgtccgct gattcgcagg agctgacgac gactgatatc tctgcctatg
     6661 cgcatgttct gtgtaccctc gtttgatgag ccgactcaag cttttcgacc ggcgagtgtg
     6721 gttgctgttg taatatgggc atcacttgag cgtcaacacg gttgcggctg agagaaccag
     6781 ggcatgacgc ttggggcggc tctccttgga cgggagcctc gccgcagcga agctggcctc
     6841 cacggccgag caggcgggcg gggccagacg acaaagcggt caacggcaaa ggacagcgtt
     6901 tcgttgcggc tacggaccac aagaagtgtc ctgaagtggt tattccccta cggtaatctc
     6961 ggtatgccca gattaccgtt ttagagtctc cctgaggcct gcgggagcga gcttgtctca
     7021 gagcagtggt cacagcgact taacgacgcc ggcagccggc agattcccca gcttgacgca
     7081 tcggggaatg accggtcacg gcaattctct cgtttccaga gcgcaagttt aggttgtggc
     7141 ggcagatagg cctccgcccg agcatccaga ccaacgcagg ataggtcgcc tggagctcag
     7201 cgagggaggg ttccacaatg ccggaagata gcccgaatca aagattcttg ttgacacgct
     7261 ttccagagtt cctctatgat gggttcatgc tgatttggcg ggggtgtata tccggccccg
     7321 ccttgctgtc gctaaagcgc gtcaacagat gctcgggaga gagacacttc ttcgagcatc
     7381 tgcggagtac tttagggtcg tcacctccaa gggttttcgt tgttagggcc gtttgtcgtg
     7441 cattgcattg tttcacctac tgaatcgaca ggctcaagag ctgcgagttg ggtcttggct
     7501 tgaacccgtg gcgggatgcg atcggcgctc aggtgagaaa cacgcgtaca gtgcgattcg
     7561 cgtgagtcgc atttcgatag gggaggttct caggaaggcg gtccaattcc ggacgggact
     7621 tgcgtatgcc ggcgagctgc gtgggcgcgc cggacccatt caacccgttt gccggaatgc
     7681 cgcgaggagg aacagcgatg agatacctgg ttgcactgtt tgtgtttggg gtcatggctg
     7741 ccggggcgtc ggccgcagtg gttgtcggtg ggacagagac tttcaccagc ggcgtcaacg
     7801 gcggcgcaga ctggggcgtc ggcggcgcat cggcctatga tgggtggcag gtggacttgt
     7861 ttaagggcta tgatagccgg cccggcaaca acgatcacaa agagtacttc ggctcaacag
     7921 gacagatcct taccgactgg ccggatggca ggaagtccaa aggccagccc gatccgggga
     7981 gccactctgg cgatccgggc acgctggtcg ttcaacacaa cgctttctgg aatggcgccg
     8041 acccgaataa aggtgaaacc gacccgccaa gcacgtgcac tcaactcaag tatatcagct
     8101 tcaacagtgt cgcaggcacg acgtacgagt tgaccgggtg ggtcaataca gtatacatcc
     8161 agcacggtac agcgtgggac ggaatgcatc ctcggatcgt cgatcacggc actcccccga
     8221 tgccggcgca gttcccggat atccagatcg gcttgaagaa cggcttggct gatgccaacg
     8281 acttggacgt cacctatacg caagtcacga atctgattga caatatggcc ggcggtgctc
     8341 cgaaccccaa tgcatgggtg cagttttcgg tgagtgcggt tggcgacggc gggcccatga
     8401 ctattctctt gaaggtcgcg gcgccggaca tcagcagcgg tgcggttcac gatacccgaa
     8461 cctgggatgt cgacgttcgc ttcgatgaca tcaccctgac ccctgagccg acggctctgg
     8521 ttctcctgag cctcggcttg ttcttggtcc gcaggcgctg acagcagccg gccactcgac
     8581 aggctctctc attcggaccc cgggtctgat gacgcccggg gtccgttttc tgcgcactgc
     8641 ttgcacgttt gtggagttgc atcggacgtt cattcggttg ggcgccccgt cgaacccagg
     8701 gcctgccgcg atggaaacgc cgcatcctcg cccgggcgcg agtaaaggta acggtgtttg
     8761 aactgctcat tgccgcggcg gcggctctcg gcctgctcgt gatccagtcg tgcaagaaag
     8821 ccatcgggca gggcgttccc acggatggcg ccgagtcgca gactccggcg tcgttcagca
     8881 tattcgatgt tcagcgttga cagccggacg tctacctctg ctgtcagttc ttcgagaacc
     8941 tcgtcaccag cgccccgtgt ctcgatatgc agcatgtaat acggcgggtc accccagcgc
     9001 ggggccagca cgaaaccggt cacgccgatc cccgtccgac cggccgcctg actcaccgcc
     9061 tgcataacct gatgctcggt gagtttctcg ccggccagcg agcagatgtg ggctcccttg
     9121 ctcaggaatt cgatcaacgg tgcctgcccc tcatatccga ctactcggac cagatcgccg
     9181 atgtggtagc gatacaaccc cgacgcggtg gtcatcaaca cgaagtactc ctgaccgacg
     9241 tcgagttcgt gacacagaaa agtcttcggg ttggtttctt cgattcgatc cggcggcacg
     9301 aactcgaaga agtggctctg ggtgtcgagg atacccgccg gcgtgtagtc ctccatcggg
     9361 atggagattc gcccctcgct ggccagcaaa ccgatgtctc gtaccggcac gtccccaaag
     9421 tatttcggaa agtcctggag atacaggccc atggtgccgc cggtccaatt ggcaatgaag
     9481 gccagattcc agtagtgctt gggcaaaagg gccccatatc gttcaacaat gccctcgagc
     9541 tcccgcgctc tctcatgatc aggtcgcagc cggtctttca ggcttgcgaa aacctgggca
     9601 ggaacaaata actcgaaaga cagcgtcccg tcatggacgt cgcggatcaa cctttcccga
     9661 tgctcgtcgg cagtgcgggc aagcttcagt tgggtggccg gattcgcggt gatcatgaag
     9721 gcgacgtcct gagcgatcgc gagcctcatg atcgcgtaat acttcgcgac aggctcggtg
     9781 atctgggcgg tgcactgggg tgcaacgtag tacttgcgga ccaaacgttt ctgggttgcg
     9841 gccatcaagc ccgtaatcgc cccgcaggga atacccgcag gggtccttga ttcatccatt
     9901 ctggacgtta cttgcacgat tccgcgcaga aacgcctggg gatggtccag aagggccttg
     9961 atgccgaaga cgttccagcc gcgacggaac gtcctcaaga attcatctgt gaccggtatg
    10021 tatttggggc ggtcggtcgt tccgctggtc agggcgaaca ttctgacgcg ttgccgcccc
    10081 ccgaacatgg cttggaggcg tccattgcag actcgttcga tgtagggtcg atgatcctca
    10141 taacgcagaa tcggaacctg ccttcggaac gcgtccactg atccgatccg atcgaagaga
    10201 tggtcgcgcc cgaagtcgct gtcggcattg cggcgaacat gcgccgcgag gacttgcttc
    10261 tggacttccg tcgctttgcg agtcgcagcc atgaaccgcc tgtaaaccgc ctcggcatga
    10321 tgcctggcga tcacgtacat gacgcggtcg gtcaatgagg gtgtcgatct catgttgcct
    10381 gtgccggacg tgcccgttat gtccccccgg acggtgcgac gtgcggtatg gcctcacccg
    10441 cggctcaaat cagtgatttg aggtggtcga gactggccgc cgcctggtcg atcgtgccgc
    10501 attccacgct gaagcagatc gtccgctgga cgggcttgag gatgtcgatg acacgtttcc
    10561 agtcgatgac cccttcaccg caagcgcatc caaccggcgt accggtaacc ttgcctcgtt
    10621 cgtcctggct ctgcttgagg ctgatgtcct tggcgtgcat atggatgacg cggtctcggc
    10681 agcgttcgag ccactcgtac gggtcggcac cggccaggta cgcgttgccc gtgtcgaagt
    10741 tgatgcccag cgacttggac ttgaccaggc cgtatattct gtcgagcccc gcgggagtct
    10801 tgctgtactg ctggtgctgc tccaggccga tcaagatgcc gcgcttttcg gcggcgcgag
    10861 tcgctagcgt cagcgtatag gtcataagaa cgtggtcggt ggcttcgtcg gtgaaatcag
    10921 gtttgggtcc ttcgtccgtg ttgacgatcg gggccgcaag ctcggcggca aaacggatcg
    10981 cggatcgcag atagttgacg ctgatatcgg gcttgcacaa cggcgagtgg gcgctgagac
    11041 ctgaacagcg aaccttatac ttctcgcaaa ttgcccgaat gtcgagggga tcctcgaaca
    11101 ttgagaccgt atggaagtag ccggcctcgc tcatcagttc ccgtccccaa tgcaccatgg
    11161 gctcgatgaa ctcgtagccg atctcggccg ccttctccac acccgcgacg aaagacatgt
    11221 cgtgatggcg gacgaactcc atgttgatgc cgatctgaat cctggccatt gctcagctcc
    11281 ttactggttc tcacgccctt tgccaaacgg gaacattcta gcaacctgct ggccgaccgt
    11341 cgaagcgcgg ggctagcgtc ccggcgggta caccgggtcg atcgcggtca ccgatgccgg
    11401 caagattgcc cactttgcca aagcgagggg tgggtcggct ggcggactcc tcagggtgcg
    11461 tgcaggctcc cgctactgca cgtgttcccg gttcttcagt gaagagccaa ggccgatgcc
    11521 ccgtgtgctt ggatgggggc cgggtgctac aatcctgggg ttgggttgca cgtgcatacc
    11581 ggagcattag cccgatgatc gccacgccca agggaactca cgaccgccac cggctccagt
    11641 gtatggagat ctggggcggc aacagggccg ttgaggacac cgtctcggtg tttggcatcg
    11701 acgcatgggt tctgagtctt ccgcatgccg aggggcagca gggcggggat atccactatg
    11761 tctcgatgtg tggcagcggg cggatcgctc ggttcatcgt ggctgatgtg gccggccatg
    11821 gcaacgccgc tgccgacttg gcggttgtcc tgcgtaatct gatgaagaag cacattaacc
    11881 gactcgatca gacaaagttt gcgcgagacc tgaaccgcga attcggtctg gtcgccaacg
    11941 atgggagctt cgccaccgcc gttctcgcca cctacttcgc tcctacgggt cacctcattc
    12001 tctgcaacgc cggccatccg cctccgttgt ggtaccggag ccgtgaccgc cgctggaggc
    12061 ccctcgtgcc caccatgccc gagcaagtga gtcgggttcg caaccttccg ctgggcgtga
    12121 tcgaaccgac ggattatcaa caattcgccg tctcgttgtc agaagacgat ctcgtgcttc
    12181 tctatactga ttcgttgatc gaagcggcaa atcccgcggg ggcaagactc ggtctcgatg
    12241 agctgctcgg tattcttgag agacttgacg gcagccgtcc ggagggcttg aaccacgaga
    12301 tagttgccgg cgtccgcaag tgggcaagcc cggtacgact cgatgatgac acgaccctat
    12361 tgctcctacg gcaaaacggc gagaggccgt cttggccgag cttctccaca accgtgaagg
    12421 tgctggggaa gctcattggt ctggtggatt actgaccacg atcatctgcc cagcggctag
    12481 agcgcctccc ctctatgctg attgagccag aaagcaatcc cgttctgtta tgcatccggt
    12541 gctgaaaagg cttgccattc gtgacgctga cggcccaaac tcgctgttat cggacctcag
    12601 gaccgcaagc gcagactgcc gcatgccagt cgagtgttga cgcaaagcat cttggtcgcg
    12661 cgcgggtgcc acatgctttt cattggtgat atctctttat aggacacaat attaggtctg
    12721 ctttcattgt attggcatag gttatcgctg ttgctcgccg acatcatcgt ggcgttggcc
    12781 cggatggcgg caattgatca aatgtgcctt tgaacaaaga tttctttggt gtaatccacg
    12841 atagaagcga agcttgggaa agccttagtg gcatcggcaa aactacccac ttccactggc
    12901 acaccgcttg ctgttagaat atgggtagag gagacgccat tccgctgtga tgttctgtcg
    12961 tggcggatgc tgaggattca gatgcgacga ccggttcttc tgtctggtct cgcgattctt
    13021 attcgcacgt tctcgtgcat gtacaacagc gaggtttggt ctaatcagcc tgcgctatgt
    13081 cgagtctgat gatcgcggtc ggctggctgg tcggtgtgaa caatcggcgc caagtgtgga
    13141 gttcgcgctg agcgatggtt atttgacgca cgaggcgccg gaggggcctc ggagcgccag
    13201 cggggaaaga gaagccggca gggaatgaag cgcattggag gatgaggagg aggggagggg
    13261 aaggaagcct tgtggcattt cacagtgatt gggttgttca atcggccgtg aaatgcgcaa
    13321 acaatgacgg ccgcagaatg cggcaatgag aggcggaagg gacgcagcgc ggcccatgtg
    13381 cgagacaacg cacgcgggcc gtgctttttt gttcggaacg tattcctgct atcaacaact
    13441 gaaaggtcgc gacgctaccc agggcgcggc gctgggtctg cgaggcccag ccgtctcggg
    13501 cgtgaagggt cctgattctt tgctacactt tgcgatgacg agtgcgagcc tcgaaagtgc
    13561 cgtccggaag cgggtgcagc tcgtagtcgc cgaacaggtt tggggcctct ttctgaagaa
    13621 cccgcagaac ctccgccgcc accttccgaa tctcaacctc ggcagactcg ctgcatcgca
    13681 tctcgatgaa gtggcgcaag gctctggcgt tggccgtgac gaagatcttg gtctccgtcg
    13741 cgtttggcaa gaccgaccgg gccgcctgtc gggcaatctt gcgctgaaga gtcttatcct
    13801 ggacctgcca aggggcgttc atcagggcgt ttaccagttc ccgataagcc tccagacttt
    13861 tttcgatggc ccgcagccag acggcatgca ggccagggtc ccgggcgatg cagtcgggct
    13921 cgacgaaatc cgccgtggat tcgtccacgt agcgctgcga gagttgggag taaccgaaac
    13981 cggcccgatg cctgatcagt tcgtgggtga agctccgtga gacgccagtg atcagcaggt
    14041 tgaacacggc gtgttccagc acactgccgt gtccgacctc caaaagatgt cccagataag
    14101 ccttgttgcc tccgggacgg ggcttggcga aactcatgta gcagacccgg ccggcgatct
    14161 cgcacagctt ctgaccggcg atatccgtgt cggtggtcca attggccacc tcgtggtcgg
    14221 ccaggaaacg gctcagttcc tctgggtcga tcgtctgccg gccgagaaga tagacctttg
    14281 gctcgcggat gattctcaag gtttgaaatc ctcttcagct ttcagaaacc cccgtcgttc
    14341 gttgtttgct cgccagggtt gctcgcggag gcgactcggg ctactctagt catctgaacg
    14401 gccttttcaa tcaggccgat gtccggaaga cgcggccctt gtgaatttct ctctgggcag
    14461 gactcaaggc gcctgtgagt cttgcgggtg tggatgggta cgcccgacat ccatggctgg
    14521 cttacggcgc gtggccacat tctgacacgg ccaagctgca tggcgcgcca aggcaggaca
    14581 cgaccagggt ggtccgcaga ccctccagtg cgaatgaggg cagatgatgg ttgcgaatgt
    14641 tcaggattac gtcggcagag tgacgctggc cgatggtgct tggggaaccg aactcgataa
    14701 gctcgggtgc ccgcctggct actgccgcga ggaatggaat atcagcaagc cggagctggt
    14761 cgagaaggtc gccgcctcgt atgtcgcggc aggctctgag atcattctga ccaacacctt
    14821 tgggggcaat cggatcgtgc ttgccagcca tggattcgag gccaggatga gagaattcaa
    14881 tgaggcgggt gcctgtatca gcaggcgggc tgccggccac agggccaagg tgttcgggtc
    14941 aatgggtccc agcggcaaga tgctgttgat cggcgaggtc gacgagaaga cgctctatga
    15001 tgtcttcagg gaacaggccg aggccctcgc ggccggcggg gcggacggct tggtcgtcga
    15061 gacgatggcc gatttgacgg aggccatgac cgccgttcga gcagccaaga ccaccggatt
    15121 gccggtggtg ggcagcatga ccttcgattc cggccgagac aagtcctgca cgctcatggg
    15181 ggtgacgccg gaacaggcgg tacagggtct gaccgaggcc ggcgccgatg tcatcggatg
    15241 caactgtgga atcggcatag ataactatat caaggtagcc ggccgcttgc gagccgcgac
    15301 cagcaagccg atctgggtca aggccaatgc cgggctgccc gaaatcgatg gcagccgggt
    15361 ggtgtacagg atgaccccta aggagtttgc ccagaaggca cgcgaggtga ttgaggccgg
    15421 ggccaacatt atcgggggct gctgtggcac gacaccggaa ttcatcaatg tgctggcggc
    15481 ggcagtgcgg tagcttttcc gttcgttcat ggccaacgag cagccgtcgg cacactcctt
    15541 cggttttccg gggccgctgg gtgcttgacg gcgggcgatc atgacgggcc tactcgtcca
    15601 cgagcaaaag caggcaacag gtttcaggag aggcggatgc ccgagtcgat tgttgatggc
    15661 acattggagt tgcaggagaa cggcggaggg ttcttgcgct ctcccaggcg caactacgcc
    15721 gtccattcga cggatgcatt cgttcctgac tcaatcatca ggcgatggca tctccgcggc
    15781 ggcgagacgg tcacaggcct gggggtcgtg aacgggcatg gcggacgttc tcgattggtt
    15841 ctgtccgccg tccaatcggt aaacggcttg gaacccgagg cctacgggga actgcccgat
    15901 ttcgccgagc tgaccgttat tgaccccacc agcaggctct ggttcgagac gcccggcggt
    15961 tcgctctcca tgcgcgttat cgatctcatg acgcccatcg ggctggggca gcggggtctg
    16021 attgccgccc ctccccggac cggcaagacg gtccttttgc agcagatggc cgccgggctt
    16081 gccgcgaacc atcccgaagt ttacatgatg gttctcttga tcgacgagcg tccggaagaa
    16141 gtgaccgaaa tgcggcggac ggttcggggc gaggtcgtcg catccagtaa tgaccagaac
    16201 gtcgccagcc acattcgcat cgcccgcctg atgatcgaaa aagccaagcg gatggtcgag
    16261 accggccgga acgtcttcat tctccttgat tcgctgaccc gcctgggcag ggcctataac
    16321 gcgggcattc gctcctcagg ccggaccatg agcgggggca tcgatatccg agcccttgcc
    16381 gagcccaagg cgatattcgg ctcggcgcgc aacatcgagc atggaggttc cctcaccatc
    16441 atggcctcgg cccttatcga aaccggcagc cgcatggacg aggtcatctt caatgagttc
    16501 aaaggtacgg gcaacatgga gatcatgctc agccgtgatt tggccaactt gcggctttgg
    16561 ccggccatcg accttaatca gtccggcaca cgcaaggagg aattgctcct gggcaaggaa
    16621 gttacggaga agacctaccg gatccggcga cagatctgca atcagccgcc gaacaaggcg
    16681 atggaaatgc tgctcgattc cctgagaaaa ttcccgagca accaggcttt tctggacagc
    16741 atgcctttgt gaactcgtgc gcggctttcc agtggtttgc cgctgcgatt cctcaatgcg
    16801 tccgcttgta tctcgattca tggtgcctgc tagccgaaga cagaacgggc ggcatgcgcg
    16861 acggtgatcg ctcgtccgat aagccgcacc tgctggcgac gaaataacgg aggcagaagc
    16921 tcacatggtg accatcgcaa ccggcgaaga actgctggcc gaactgcggc ggcatttcag
    16981 actggtggag atcggaaatg actggccggg ttcctgcaac cggcggcgac atacgcgata
    17041 tgagcttgaa tcgggctcgg tgcccgtcga ggtttgttcg cgcttcggat tgaccgcacg
    17101 cggcttgttg gccgacatcg tggtcggcgg tgcgagggtg atcaccgact atgtgcctgc
    17161 tatcggtgag attctttcgc tctcgttcaa tgttaacaac aacttttacg ttctggacgc
    17221 tgaagtgcgc catgtgggcg tcgagggttc tattcgttac gtcggcgtcc ggttcgtgga
    17281 gtagcctctt gccgtggcga cggccgagcc gcttgagcgg agagaacgca gggggatgcc
    17341 ggtggctcac tcgaacatcc gacttctgat catcgacgac agcacgtttg ttcgaacgat
    17401 cctgcgaagc gcgctgtccg gccaacgtaa catcgaggtc gttggtttcg cctccggctg
    17461 cgaggaggtg atcgagaaga tcgatcacct gcagcccgac gtggtgaccg tggatgtcga
    17521 catggttgac cggcagggtc ttgagatcct gaatctggtt cagtccagat gctctgcgag
    17581 gatcgtggtc atcgcgtcgc tgaaccggga aagcgcgagc atggccatgg aggcgctgga
    17641 acgaggggct tttgattttg tcgtcaagcc gaggaagacg ggcgttgatg gcattccgag
    17701 attcagggaa ctgctctgcg agaagattct cgccgcagtg cgtgccgcga aatcgcgggg
    17761 gtctcgcggg cctttggccg acgtcccgga ggagctgatt gcgagcggaa tcaagggccg
    17821 gtgggtggtg ggaatcggga tcggcaccgg cggcccccag acgctggcga gaatgttgcc
    17881 gtcgtttccg tcggatttcc cgcctgttct ggtcgcccag cacatgccgc cttatttcag
    17941 caccgttctg gccgagcggc tggccgcgac gtgccggatg gttgtgcggg aagcggtcga
    18001 tggacagccg gtcaagaccg gcgagatcct gatcgcacca ggaggcctgc acatgcaggt
    18061 cgcccgccgg ggtgaggagc tcatcgttgt attggagggt ggtcctcggg tgtccggcca
    18121 tcgccctgcg gcggacttga tgttcgagtc catcgccggc gcatgtggtg ccaaggcggt
    18181 cggggtgctc ttgaccgggg cgggtcgtga cggggttgcc ggtctcgcga agctgcgtga
    18241 acacggggca tggaccgtgg cacaggatcg tgccaccagc tatgtatttg gcatggctgg
    18301 cgcggccatc agagccggtg tcgttgacca ggtgttgccg ctcccaaaga ttcccagggc
    18361 gattgtatcg ttgatggctc agggctgccg cgaaacggtc acggcgtcgt aatttcgtct
    18421 tgtcgaccta atgccagatt gcttacgtcg tatgccaagt catcatacgg caacctaacc
    18481 tccggaacaa ccagattagc ggcaggttgt cttagactaa ggcggcaagg tcgtcgatat
    18541 cttgatttga ggtttatagg gaattagact gaggccaagg caacccttgc gggcgtggcc
    18601 cctgtcttgc cgtaagggtt ccttccgtca ggatttaaga tgatagaccg actagccgta
    18661 aaattatttc cggaacgtgg gcattcggtg gtaatgctac ttgcctgctg catagcgttg
    18721 gcgttttccg acgttcccag agaggctgac ggggcgggac tcactgaggg gagccgggtt
    18781 gcgagttatc cggtgaacac ggtgattcct ccgggcacgc aaggtggtgg gcgtgccgtc
    18841 tttatggggc aggctgtgcg gccgatccag ggcggaaccg ccggtggtct gggacagaac
    18901 tggtatcgcc cgcagggggc aaccgcactg caacggctgt atcgggaggc ggagacaaag
    18961 ggcccgctgg ggctggaacg aaggccgagc aggtgggact cgcggagcag gtattcaggc
    19021 atttcagctc tttctggatc ctccgaggtc gaggtgggaa caagcgtcgg ggcgggaact
    19081 cccgaccggc cacgggtcac agactcgcgt ccggcggaat tcgaaatcag ccgtccaacg
    19141 ggggccctgt cgcaagccgg cctggcggca aggcgggcag cctacctgga tgcgggctgg
    19201 gcattattca aagagggaaa at
//
LOCUS       DAOTPW010000101        19187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2915_length_19187_cov_5.274043, whole genome shotgun sequence.
ACCESSION   DAOTPW010000101 DAOTPW010000000
VERSION     DAOTPW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19187
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2915_length_19187_cov_5.274043"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="P5316_10755"
     CDS             <1..597
                     /locus_tag="P5316_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85477.1"
                     /translation="PHGLPGHVWALYWPVVTAVVGAVLVVVANLVARTEDWQALYEPA
                     YLSGVVVMPLSSIAGVLLGNPQLIPSWVPPLTFGCLVILYLLASLLVGPRTFAAVAVL
                     CGGMCFWKLQDATGWTNIAAPYYYAMLIFLSAGLWAWVAHERQPARVLRVLRWAGIAL
                     AVASIVAGLGASKWGGGKSRIRGGTAEPVIFGGSQGGA"
     gene            690..1139
                     /gene="dtd"
                     /locus_tag="P5316_10760"
     CDS             690..1139
                     /gene="dtd"
                     /locus_tag="P5316_10760"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRR85478.1"
                     /translation="MRAAVQRVHEASVTVDGQVVGSIRQGLLVYAAVAIDDTEADARY
                     IAEKVAGLRIFNDPDGKMNLKVSDVGGGLLVISAFTLQADARKGRRPSFDAAAGPDLA
                     LVLYERLCEMLGDMGLSVAKGVFRAHMEVASINDGPICVLLDSKRTF"
     gene            complement(1407..2747)
                     /locus_tag="P5316_10765"
     CDS             complement(1407..2747)
                     /locus_tag="P5316_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85479.1"
                     /translation="MTRRDFTKSVAGAGLMAAAGPALGALGANDRIRLGIIGVGNRGD
                     QLIDAFKPHADAEFAAICDVYDPYIEFAKNKVGGNPFTTKDYREILERKDIDAVVIAT
                     PDHWHALQFIHACQAGKDVYVEKPLSLTVREGRKMVEAAAKYQRVAQMGNQRRSSPAH
                     RQIIDMIQAGKIGKVTAARCFHIRNEAPLGIGNPANSEPPKGLDWDMWLGPAPKVPYN
                     ENYCFYKFRWFRPYSGGQMTNFGTHYMDVIQWALGDICPAGVFAVGSRVGVEDNRGVP
                     VTMEAVWDYPDGAIVTFSQYNANSAPGTAKGSAVEIRGTRATVYMSGGNAQVVPEDWL
                     TKPLPALNPLDREVNKRLENAVEKGTEGLSIKDSDSTREHARNFLDCVKSRQTPNSPV
                     HIGHRSTASTLLANIAMDLKKYLTWDGENDRITNDPDANRHLGYAYRPPWNLPA"
     gene            complement(3520..4752)
                     /locus_tag="P5316_10770"
     CDS             complement(3520..4752)
                     /locus_tag="P5316_10770"
                     /inference="COORDINATES: protein motif:HMM:NF023375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3466 family protein"
                     /protein_id="HRR85480.1"
                     /translation="MTNQPKHSAQRQVTLPPATGGGRFRLRLFISIVFATTCMPGATR
                     TAQAECYAVTNVGTVAGATELQVRDMNNAGEIVGWAVANNVYQGFLYANGLMISLGSL
                     GGQGCQAHGINDARQVVGAAATATGQEHAFIYTGGQMIDLGTLGGTRSSAAAINNSGL
                     VTGFSTAGNGTYRLFTWQNGVMTDAGLVGLTNCEVWDINSSGTVIGLNQLITSQLLAF
                     NWTSSGGFQTLGTFGGAEGYPYRINDNGQIVGAIWTGQTTLLGARIYHAFLYRNGATT
                     DLGTLGGSSSGANGINNSGAVVGWSQVSTGQHAFIYDISNGMRDLNSLISPTSGWVLE
                     AATVINDAGQIAGVGRLNGARRIFLLTPSQTDADDNGLIDACEARSAPDTSDSCGAGL
                     PITLATVFISLCFMKRRD"
     gene            5006..5497
                     /locus_tag="P5316_10775"
     CDS             5006..5497
                     /locus_tag="P5316_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRR85481.1"
                     /translation="MEALRALAARRSIRRFKNQPVSREAILRLIDAARLAPTARNVQP
                     WEFIVVTNPNRLRQLAALTDHGKFIADAPVCIVVCSRDTKYYLEDCSAATTNLLLAAT
                     ALDLGACWVAGDKKAYASQIVSLCGAPPELKLVSLVAVGYPEEIPSPPKRTIEDVLHW
                     ETF"
     gene            5589..9545
                     /locus_tag="P5316_10780"
     CDS             5589..9545
                     /locus_tag="P5316_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR85482.1"
                     /translation="MILGSIPRCARSLKGATVLAVSVFARLAIAEPVEWKPVFEDDFE
                     RAVAGSKYEVGEDIATIVEGALRLAGQEKPVLVAQLVSRDVRVEFDARPLPDAPPGIV
                     GCQLHSSTGDHYRLEVDLAENGTARITGPGLDLTKERAATRIQPGRPFRFLAQREGKQ
                     FTLKVDGVIVLEGAVEKVVGSSGIQRVGLIAEHGMLVDHLRIYSRVPPHPDCCPPPLP
                     WLPLTRRNAEVVLSPGQASTPALAEALALLNDRRFAEARARFEAMDDVTLRLTGLAFI
                     LGHLDYFEKPVYGDLIDRKDGGEYTQRQVVEYFGEFGSFAARWREELGRHPDNEILRL
                     YAPFVEHFGRLCLNRWDGPRHAKALADLDERVNPFSHKASLYAARFDYWQAMEGGDER
                     AKAQCRARMKRLLSLYPDNRILREYVGDPVPWGEPLNADTDRHPAWAAYLREAYARES
                     VIVERIFECRQRSDGQCGGGYGDDVEMLRKIVPIAAISTANRRIIEGVERLAEGVWKY
                     DTVEGYNPDVSDVEHSAEQSADSFPTMMLLRYGDARFYEFNLRSAKTIRERFMGLDAQ
                     GHPRFLSGTFGSRGVATHERAGGDTGYHARAMKHFLWLAWYGNEEALDWYVRWLDGWR
                     EAAMADAPDKPSGVLPGTLWFPSGHWQHPWSGKPWYAEDPTHLYGPWGQGGMLQESFL
                     NVYALTGDAEFLKPMQLLMDWASLGPLQRDRERDQLQPGSREWFRLPMAHSAGANLTA
                     LYRHLTGERVYDEYTMRFGTAPQLYPIDHDLNRLMSGIEKAAKSLRTNLWYWTTETLS
                     TDRLHLPAVAEVWSAYTGAINTTGDCELPTWAVTYETPDTDFAALVIDTTPRRLRVWL
                     YSFREEATPIGLRVWQLIPGRYVLNAGEQLKGEFPFQHRYAWAPSCRVDIRTRGQVVP
                     ITLPPQKVYVLDLRLEEAMPVPQRASDLALHARDITVQGKQVIVDVHNIGNAAAKNCA
                     VAVQRQEREAWTTLGVQRVPRISQPVNLSPYLERLIFDVPGLDLTAAYRIVIDPENEI
                     DEICETNNAAQLLPDRVGPVAFRLPEEGEAVVPLPPLLQLDLGDGLTIELVLIPAGEF
                     MMGLPNNEPRAGAYERPPHRVRIDHPFYMGKYEVTQAQWKRVMSGQNPSHFQGDDNLP
                     VEMVSWHMAHDFCERLSRRVRRSVRLPSEAEWEYACRAGSTSYWSFGDEVQDLGDHAW
                     YGGRDVEGRTHPVGMKKPNAWGLYDMHGNVREWCADWFEPYPGTKAPPTEFAGPRQRI
                     LRGGSWDWVEPENQGSARRDRALPDARRDHYGLRIVVSAEGAPSTAGPVVVTTRGQ"
     gene            complement(9686..10927)
                     /gene="tyrS"
                     /locus_tag="P5316_10785"
     CDS             complement(9686..10927)
                     /gene="tyrS"
                     /locus_tag="P5316_10785"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRR85483.1"
                     /translation="MSFATVEEQLRVLTRGVERIDTLDELRQKLQRSRQTNTPLRIKL
                     GLDPTAPDIHLGHTVVLRKMRQFQDLGHKAVLIIGDYTAKIGDPSGRSKTRPVLDDKA
                     IEANAKTYFEQAGKVLDTSPDRLEVRPNSEWLAGMTFADVLKLAGQMTVGQMLKREDF
                     RKRFEAEIPIGVHELLYPLMQGWDSVCIQSDVELGGTDQTFNNLVGRDLQRGAGQEAQ
                     VVMVMPILVGLDGVEKMSKSLGNYVGVSESPHDMFGKLMSLPDGCMANYLTLLTSIPD
                     GEIRDLTDASKTHPMEAKKRLAVEVASAFHDRKLVQQARAEWEQIHQKKASTGDLVVP
                     ADTPTIRVPDAVMQDGRAMTLKLIVACGFAATNSEARRLIEEKGIRLNGELVEEATGT
                     LAVKTGDILQRGKRKFVRLTV"
     gene            11222..11662
                     /locus_tag="P5316_10790"
     CDS             11222..11662
                     /locus_tag="P5316_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRR85484.1"
                     /translation="MSSEIYAQAGAIPYRHDSNGRLEILLIRRIDKERWGIPKGLIDP
                     GVSPRETARAEAIEEAGVAGVLSEQPVGFFSFNKKEWDAMVHVTVFLLRVTEIQPEYP
                     EQYRREREWFAFNTAVEVAGRKGLRELLLDVPKLIKEHLPETHE"
     gene            complement(11673..12842)
                     /locus_tag="P5316_10795"
     CDS             complement(11673..12842)
                     /locus_tag="P5316_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85485.1"
                     /translation="MKVVWQVAGGLCVLAAALWWTCAGRGDGGRDSAGRSADRGGKAV
                     TPPLPRRPVSALAGVPYHGMSWQVHYSATAIQQAKVLLREIADLGADTVMISNPGYQE
                     HAGSDSFKIDPVVTPSREQWLEIFKIAHDNGLRVILMPIILLSDPRGNEWRGVISPPN
                     WDDWFEQYRKFVLHFARIAEEGKVEVFMVGSELVSTEKFTNRWYEVIREVRREFSGKL
                     SYSANWDHYKVIEFWDELDLVGMTSYYKLSSEPRPSLHNLIKAWEPLKKGLLKWQATV
                     NKPLLFTEAGWCSQEGASIEPWNYYYKQEATPAGLEEQLNCYLAFMETWKYSDEPGKS
                     LTPDQLGGVLWWEWNDTPGGKDDYNYTPRGKPAERALRDWFAAARQRWPATSPAK"
     gene            complement(12907..15129)
                     /locus_tag="P5316_10800"
     CDS             complement(12907..15129)
                     /locus_tag="P5316_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85486.1"
                     /translation="MMIRRAPMCLLLCLLPAGCAVWKSDRVAIDDLLHRPDARQGTLG
                     PTGKRLFTERLDPGLGFRGQFSYIHYAKIADPIVFQPLDRAISSSNHNIFKQDKPFVS
                     ATDRVDWFPSHLATHSRFGRFVVIERKFITDDDTLVDLVTVTNDSKVSAECLLHVSSG
                     LARERGPDNTLTGSADFHGVRGHAVLLAPRFTLASKQSSLWRRVNIRPGRSVSLRIVM
                     SFQEDAAKAAQTAREWASRPNALPHHKAVYQKWFDDNCPHFECDDPYITKLYWYRWFV
                     ARHCLSRARTGNLPYPYFFEGTHQPHFPRLISFSSPHIIAETRWLRDPQYAFGQVQNH
                     CLNPDDTNKFFISARINEKGGDYNNWIVKAAWEAFKVHPDRRWLQSVIEPMSEDVRGT
                     LRQFDRDGDFLPTPRNHGTTGMEFQPSFFYFNENYDNSKPDARLERGDYAAYLYGNAT
                     ALAEAWRFLEDEARAAEFQQLAGKIRAACLEKLWNPTDRFCYAAREHDGAVARVREIV
                     GFYPFMSRLFPNQAEYVASLAYLVDPTEFWTAYPPATVSRKCPAYTPHVATWPAAGGK
                     THGCMWNGPAWPHATSMILDVMAIALRDYTQPHVKPEHFWHALDRYTHLQYENGDLAR
                     PLVTEYYDGQSGSPDPTGCPDYFHSTYCDLVIRHLVGLQPSNSDRAVIDPIPGPLKWF
                     RLRRLRYRGHELDIVYNSADAASAAGLTVWVDAKQVAHRAGKLGQVEFDLRAAQSARM
                     "
     gene            complement(15197..16006)
                     /locus_tag="P5316_10805"
     CDS             complement(15197..16006)
                     /locus_tag="P5316_10805"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRR85487.1"
                     /translation="MKKLHWMLFGGLALCVSGALVCLAQGESAKKKKILFYSQSFGFR
                     HSVVNRPLTGEMAHAEKVFKEIATKAGYEVFLSQDFHDLEGDACKQYDAIVFYTSGNP
                     LISREPLMKWIRDGGAFIGIHSATDSFRGEDKGIPGWPEYVKMIGAAFKTHHQQRVAT
                     LKIDVPDHPATKMLEQGWKLQDEYYLFGDTFSAENSRLLISVDTQKTPEADLEAMGMK
                     PGSLVPIAWTRTEGKGRVFYTSLGHREDVWTNPAYQKHLLGGIAWAMGLEK"
     gene            complement(16220..17356)
                     /locus_tag="P5316_10810"
     CDS             complement(16220..17356)
                     /locus_tag="P5316_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85488.1"
                     /translation="MSKPLNVGLIGCGFMGRTHSNAYRKVYNFFRTPLRPVLKAICDV
                     NADKAKTFADTWGYESVETDWQKLLDRKDIELVDICVPNNMHKEIAIAAAKAGKMIIC
                     EKPLALNVAEGQEMVAAVEKAGVPNLVSFNYRRVPAVTLAKNLIDQGRLGRIFHYRAV
                     FLQDWTISADLPQGGDALWRLDVKAAGSGVTGDLLAHCIDTAIWLNGPIKSVTAMTET
                     FIKERKHTQTGKVEPVGIDDACAFLARFANGSLAVFESTRYARGHKALYTFEINGEKA
                     SIAWDLHDLHRLSYFDHRDESIIRGWRSIHVTDGDMPYMNKWWVPGLQIGYEHSFVHQ
                     AADFLEAAAKGQSAAPTFRDALETQKVCEAVLDSARTGTWVEIA"
     gene            complement(17917..18429)
                     /locus_tag="P5316_10815"
     CDS             complement(17917..18429)
                     /locus_tag="P5316_10815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRR85489.1"
                     /translation="MTYQRFEDLPVWQAAMELAVRVFTLTEDRAFANKGDLRNQLQRA
                     AISVGNNIAEGFERGTTQELLTFLYIARGSAGEVRSMLLLTERLPHFTHLKSEISDLN
                     SLVESISRQIRGWADSLQNSPITGQRYLNEPVRQQYQHQRRSSEFWQKLEADHRRRME
                     ERAAGEASEG"
     gene            complement(18545..>19187)
                     /locus_tag="P5316_10820"
     CDS             complement(18545..>19187)
                     /locus_tag="P5316_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRR85490.1"
                     /translation="DSACGPDAWYDDPAGSTRKIIDTFKEACKVAEDHGERLAAEGEV
                     CWAGMHSWKAMLAVLEGVNQPRTLGFQADMAHTLHYLLGTNAPEDRILPENFSWSDTT
                     TFTQAYKTLTSALRPWTIDFHVAQSDATVKGSGSHDKTGRHCLPNDPNGKLDIVREAG
                     LWLKDDKGQPTRKFKHICWDGCMFPNATMMKPDTWNSILAAMAAVRDTHGWNG"
BASE COUNT         3542 a         5603 c         6100 g         3942 t
ORIGIN      
        1 ccgcacggtc tgccgggtca tgtctgggcg ctctactggc ccgtagtgac ggcggtggtc
       61 ggagcggtct tggtggtcgt tgccaacctg gtggctcgaa ccgaggattg gcaggctcta
      121 tacgaacccg cttatctgtc aggcgtggtt gtgatgccct tgtcgtcgat cgcgggagtt
      181 cttcttggaa acccgcaact gattccgtct tgggtgccgc ccctgacgtt tggctgcctg
      241 gtgatcctgt acttgctggc gtcgctgctt gtcggtccca gaacatttgc tgccgttgcg
      301 gtgttgtgcg gaggaatgtg tttctggaaa ctgcaggacg cgacgggatg gacgaatatt
      361 gccgcaccct attactatgc catgctaatc ttcctgtcgg caggactgtg ggcatgggtg
      421 gcccatgaaa gacaaccggc gcgggtgttg cgtgtgctcc ggtgggccgg gatcgcgttg
      481 gcggtggcct ctattgtggc ggggctgggc gcctcgaaat ggggcggcgg caagtcgagg
      541 attcggggtg gtacggccga acctgtgata ttcggcggat cgcaaggcgg tgcgtgaacg
      601 gcccggcttg gcggaatgca gggagccctt atgcgaattg atatgcggcc ctattgaccc
      661 ccgggggcgg gtgggcggag tgaaagcaga tgcgagcggc ggttcaacgg gtccacgagg
      721 cgagcgtgac ggttgacggc caagtcgtgg gatccatcag gcaggggctg ctcgtttatg
      781 cggcggttgc cattgatgac accgaggccg acgctcgata catcgccgag aaggtggccg
      841 ggcttcgcat tttcaatgat cctgacggca agatgaatct gaaggtttcc gacgtgggcg
      901 ggggactgct ggtgatcagt gcttttactc tccaggcgga cgcccggaaa gggcgccgtc
      961 cctcattcga cgcggccgcg gggcctgatc tggcgctagt cctctatgag cgtctctgcg
     1021 agatgcttgg cgacatgggc ttgtcggtcg ccaagggcgt ctttcgggcg cacatggaag
     1081 tggcctccat caacgacggc cccatatgcg ttctgctcga ctccaagcga acattctgac
     1141 gggaagaacc gcagggattc cgcgggtggt tcggtggatg acttgtgggg tgctcgagca
     1201 tgcggcgagc gcaacttgcg gatatcgacg tgggcccgat tctctgtcta ccggtcgcga
     1261 tgaggcaagc ggggcctgca cttctcgcgg cgtccggtga ttcattcaat aggtcaggtg
     1321 atgtcacggc ggcgtcaaac gattacagcc gttgcatcaa caacccggtc gattcctgcg
     1381 gaaaggcgta ccactggtgg tggcgcttac gccggcaggt tccacggggg ccgatatgcg
     1441 taaccaagat gccgattggc atccgggtcg ttggtgatgc gatcattctc cccgtcccaa
     1501 gtcaggtatt tcttgaggtc catggcgatg ttggccagga gtgtggaggc cgtcgatctg
     1561 tggccgatat gcaccgggct gttgggtgtc tggcggcttt tcacgcagtc gaggaaattg
     1621 cgagcgtgtt cccgggtgct gtcgctgtcc ttgatcgaaa gcccctccgt gcccttctcg
     1681 acggcgtttt ccaagcgttt gttgacctcc cggtccaaag gattcaatgc cggtaggggc
     1741 ttggttaacc agtcttccgg caccacctgc gcgttgccgc cgctcatgta aaccgttgcc
     1801 ctggtgccgc ggatctcaac cgctgaccct ttggccgtcc cgggggcgct gttggcgttg
     1861 tattgcgaga aagtgacgat ggcgccgtcg gggtaatccc agactgcctc catggttacg
     1921 ggtacgccgc ggttgtcttc caccccgaca cggcttccga ccgcgaacac gccggccggg
     1981 caaatgtcac ccagtgccca ctggatgacg tccatgtagt gcgttccgaa gttggtcatt
     2041 tgaccgccgg agtagggtcg gaaccagcga aacttgtaga agcagtaatt ctcgttgtac
     2101 gggactttgg gggccgggcc caaccacatg tcccagtcga ggcccttggg cggctcgctg
     2161 tttgcaggat ttccgatccc caggggagcc tcgttgcgga tgtggaagca acgggcggcg
     2221 gtgactttgc cgatcttgcc ggcttgaatc atgtcgatga tctgtcggtg ggccggcgag
     2281 gaacgtcgct ggttacccat ctgcgccacg cgttggtatt tcgcggcggc ttccaccatc
     2341 ttgcggccct cccgaacggt gagcgaaagg ggcttttcga catacacatc cttgccggct
     2401 tggcaggcgt gaatgaattg cagggcatgc caatgatcgg gcgtggcgat cacgacggcg
     2461 tcaatgtctt tgcgttcgag gatctcgcga tagtccttcg ttgtgaaggg attaccaccg
     2521 actttgttct tcgcgaattc gatgtagggg tcgtacacgt cgcagatggc cgcgaactcg
     2581 gcgtccgcat gaggcttaaa ggcgtcaatg agttggtcgc cacgattgcc gacgccgata
     2641 atccccaagc gaatacgatc gttggctccc agtgccccca aagccgggcc tgcagcggcc
     2701 atgagaccag cgcccgcgac actcttggtg aaatctcgtc gagtcattct gctttccatg
     2761 ttggtcacct caaccgcttt ttcctcatga ttgcgaagcg aagcgtccat ggtaatggcg
     2821 cggctgcagg acggcaaacc atcgagtcgg gttcacggca gcaggtgcta cgagcaatag
     2881 aagcttgtga gttatccttg cggcatggtg cgcacaagca agcaaaagct cttttttgac
     2941 aagaggttgt tggcctcttg tgctctcaga attgtccgga cagaaagaga aagtcacagc
     3001 tcgcaggatt ttggaattgg catccgtaaa aaaggcgtgc ggcaccctgc aaacattggc
     3061 gcggtcaagg gtgccgcagc gagtggaagc ttggctacgg atctaatcga caagctgcat
     3121 gttgcttggc gactgacctg ttgattatat gtcttttcgg ttggccgcgt ccacattcgc
     3181 agtcccgttg aagtgttaaa cgccccaaag cgcaacccgt ggtgcgccta tgacttcttc
     3241 gggggttcgc gcgaaggtgt ccgcccgtgg ccgccctagc cgggtttcgg gctgtttgat
     3301 ccgtgcagct cgaaacccga cctcctacga taccatagca attccgggac cataaggcaa
     3361 gcgggaaaca cgtaattccg taaggctttt cacaacaaga gctaatacct atagactagc
     3421 ttgcgtcaca gtctaaggcg tgatgtccgg aagcaacgtc gggccgtagg ccgggcgctg
     3481 gatggcggtt tggtccaggc ggtcggtatg gtcttaaggt caatcccggc gtttcatgaa
     3541 acagaggctg atgaagacgg ttgccaaagt gatcggcagg ccggcaccgc aagaatcgga
     3601 agtatctggc gcggatcggg cttcacatgc gtctatcaac ccgttgtcgt cggcgtcggt
     3661 ttggctgggg gtcaggagga agatgcgtcg ggccccgttg aggcgtccga cgccggcgat
     3721 ttggccggcg tcgttgatta cggtggctgc ttcaaggacc cagccggagg taggggaaat
     3781 caggctattg aggtcccgca tgccattact gatgtcgtaa ataaaggcat gctggccggt
     3841 ggagacctgc gaccagccca cgacagcgcc gctgttgttg atgccgttgg ccccgctgct
     3901 tgatccgccg agcgttccga ggtctgtcgt agccccgttc cggtagagga acgcatggta
     3961 tatccgggct cccaaaagtg tggtctgccc ggtccagatg gctccaacga tctgaccatt
     4021 atcgttgatt cggtagggat aaccttctgc gccaccgaag gttccgaggg tctggaagcc
     4081 tccggaggat gtccaattga aggcgaggag ctgagatgtg atcaactggt tgaggccgat
     4141 gactgttccg ctgctgttga tgtcccagac ctcgcaattg gtcagcccga caagaccagc
     4201 gtcggtcatg acgccattct gccatgtgaa aagccggtag gtaccgttgc cggcggtgga
     4261 gaaaccggtg accaagccac tgttattgat ggccgcggct gagcttctcg tgcctcctag
     4321 cgttccgagg tcaatcatct gacctccagt gtagatgaag gcgtgctcct ggccagtggc
     4381 cgttgcggcc gcaccaacga cctgacgcgc atcgttgatc ccgtgagctt ggcaaccctg
     4441 gccaccgagc gacccaaggc tgatcatcag gccgttggcg tagagaaagc cctggtacac
     4501 gttgttggcg actgcccacc cgacgatctc accggcattg ttcatgtcac ggacttgaag
     4561 ttccgtggca ccggcgaccg ttcccacgtt ggtcaccgcg tagcactctg cctgagctgt
     4621 ccgggtcgcc cctggcatgc aggtggttgc aaatacgatg cttatgaaca ggcgcagcct
     4681 gaatctcccg ccacccgtgg cggggggaag cgtcacttgg cgttgcgcgg aatgcttggg
     4741 ttggttggtc atagatgtcc tcagggtgcg atgcccgcca aggccggcgt gcgacgttgc
     4801 cgcccctcgg caaggagagt tcctcctccg ccaaaccgtt caggttcatt atagagccca
     4861 ggaagagacc tcaacagggt ccatctcggg cggctttggc aggggtgcca ataggttact
     4921 ggcaattcga ccgtaggggc atatgatggg gcgtcaggag gcggcaaggc gtttgctgcc
     4981 gacggcctcc cagaaagggc tagccatgga agcactccga gcgttggcgg cgaggcggag
     5041 cattcgacga ttcaagaacc agccggtttc gcgggaggcc atccttcggc tgattgacgc
     5101 cgccaggttg gcacccacgg cccgcaacgt tcaaccctgg gagttcattg tggttaccaa
     5161 ccccaatcgg ctgcggcagc ttgcggccct gacggaccat gggaagttca tcgccgatgc
     5221 ccccgtatgc atcgtagtat gcagtcggga caccaagtat tacctggagg actgcagtgc
     5281 cgccacgacc aatcttctcc ttgcggccac ggctctggat ctgggggcct gctgggtggc
     5341 cggtgataag aaagcctatg cctctcagat cgtgtcgttg tgcggggcgc ccccggaact
     5401 gaagttggtt tcgcttgtgg cggtcggcta ccctgaggag attccctctc cacccaagcg
     5461 cacgattgaa gatgttctgc actgggaaac cttctgaccg gcaactcggc cggccctttc
     5521 ggtgaggcca gcgagaagcc ggcgacataa ccgtgagacg ttctggaatc caagaaggag
     5581 gtgtgggttt gattctggga tcgatcccgc gttgtgcgcg cagcctgaag ggggcgacgg
     5641 ttcttgcagt ttccgtattt gctcgattgg ccattgccga gccggtcgaa tggaagccgg
     5701 tgtttgagga cgatttcgaa agggccgttg cgggttcgaa atacgaggtt ggcgaggata
     5761 tcgcaacgat cgtggaaggc gcattgcggc ttgccggtca agagaagccc gttctggtgg
     5821 cacagctcgt gtctcgtgac gtgcgtgtgg agtttgatgc ccgccctctg cccgatgctc
     5881 cgcccggaat cgtgggctgt cagcttcact cgagcaccgg agatcattat cggctggagg
     5941 tcgacctggc cgaaaacggc accgctcgca ttacaggacc gggactggat ctgactaagg
     6001 aacgagccgc cactcgcatt caaccgggcc gcccgtttcg atttcttgct cagcgagagg
     6061 gcaaacaatt cacgctgaag gttgacggcg tcatcgtact ggagggtgcg gttgaaaagg
     6121 ttgtgggttc gtccggtatt cagcgcgtgg ggttgattgc cgaacatggg atgctggtgg
     6181 accatctccg catctattca agggtgcccc ctcatccgga ttgctgcccg ccgccgctgc
     6241 cctggctgcc gttgacgcgt cgaaacgcgg aggttgtcct ttcgcccggg caagcctcaa
     6301 caccggcgct ggcggaggcc ctggctttgt tgaacgaccg gcggttcgca gaggcccgtg
     6361 cccgttttga ggcgatggat gacgtgacct tgagactgac cggcctggct ttcatcttag
     6421 ggcacttgga ctacttcgaa aaaccagttt acggagacct gatcgaccga aaggatggcg
     6481 gggaatacac tcagcgtcag gtggtcgaat acttcggcga attcggatca tttgccgcgc
     6541 ggtggcggga agaactcggg cgccacccgg acaacgaaat actcagattg tatgcgcctt
     6601 ttgtcgagca cttcggtcgg ctgtgcttga accggtggga cggacctcgt catgccaagg
     6661 cgcttgcgga cctggacgag cgggtcaacc cgttcagcca caaggccagc ttgtatgcgg
     6721 ctcgattcga ttactggcaa gcgatggaag gcggtgacga gcgggccaag gcgcagtgtc
     6781 gagcacggat gaagcggttg ctgagtctgt atccggacaa tcggattctt cgggagtacg
     6841 tcggcgatcc ggttccatgg ggtgagccgt tgaacgccga cacggacaga catccggcat
     6901 gggcggctta tttgcgtgag gcgtacgccc gcgagagcgt catcgtcgag cggatttttg
     6961 aatgtcgtca acgatcggac gggcagtgtg ggggcggcta cggcgacgat gtcgagatgc
     7021 ttcgcaagat tgtcccgata gcggccattt ccaccgccaa ccgaagaatc atcgagggtg
     7081 ttgaacgtct ggccgaggga gtatggaagt acgatacggt cgagggatac aacccggacg
     7141 tgagcgacgt cgagcactcg gcggagcaaa gcgcggacag tttcccgacg atgatgctgc
     7201 ttcgatacgg cgatgcgcgg ttctatgagt tcaacctccg ctcggccaag acgattcgcg
     7261 agagattcat ggggctcgac gcccaaggcc acccgcgttt tctctcggga accttcggtt
     7321 ctcggggcgt ggcgacacat gaacgggccg gaggcgacac gggttatcat gcacgagcga
     7381 tgaagcattt cctgtggctc gcgtggtacg gcaatgagga agccctggat tggtatgtgc
     7441 ggtggctgga cggctggagg gaagcggcca tggccgatgc cccggacaaa ccgtcgggag
     7501 tgcttccggg cactctctgg tttcccagcg gccactggca acatccatgg agcggcaagc
     7561 cgtggtacgc cgaagatccg acccacctgt acggtccctg gggtcaaggc ggaatgcttc
     7621 aggagtcgtt tctgaacgtc tacgcgctga cgggcgacgc ggagttcctc aagccgatgc
     7681 aattgctgat ggactgggcc agcttgggtc cgcttcagcg tgatcgggag cgagaccagc
     7741 tacagccggg ctcgcgcgag tggtttcgtc tgccgatggc tcattcggca ggggcaaatc
     7801 taaccgcgct ttatcgacac ctgacggggg agcgtgtgta cgacgagtac acaatgcgtt
     7861 ttggcaccgc gccgcagctt tatccgatcg accacgatct caatcgtctc atgtccggca
     7921 tcgagaaagc cgcgaaaagc ctgcggacca acctgtggta ctggaccact gaaaccctgt
     7981 cgaccgaccg tctgcacttg ccggcggtgg cagaagtgtg gagcgcctac accggcgcga
     8041 tcaacaccac gggcgactgt gagcttccta cctgggcggt gacttacgag actccggaca
     8101 cggatttcgc ggccttggtg attgacacga cgccccggcg attacgcgta tggttgtatt
     8161 cctttcggga agaggcgacg ccgatcggat tgcgagtctg gcaactgatt ccgggacgct
     8221 acgtactcaa tgcgggcgaa cagttgaaag gggaattccc attccagcac cggtacgcat
     8281 gggcaccctc atgccgcgtt gacattcgaa cgcgcggcca agtcgttccc attactctgc
     8341 ctccccaaaa agtatacgtg ctggatctgc gacttgagga agcgatgccg gtaccgcaac
     8401 gcgcaagcga ccttgccctt cacgcgcgtg atataacagt tcagggcaag caggtcattg
     8461 tcgatgtcca caacatcgga aacgccgcgg ccaagaactg cgccgtagcc gtgcagcgcc
     8521 aggagaggga ggcatggacc actctggggg ttcagcgcgt gccgcgaata tcacagccgg
     8581 tgaatctgtc accgtatctt gaacgtctca tcttcgacgt gcccggcctg gacctgacgg
     8641 cggcttatcg gatcgtgatc gatccggaaa acgaaatcga cgagatctgc gagaccaaca
     8701 atgcagcgca actgttgccc gatcgggtcg gtcccgtcgc ttttcgtctg cccgaagagg
     8761 gcgaggctgt cgttcccttg cctcccctgc tgcagcttga tctgggcgac gggctgacca
     8821 tcgagctggt cctgattccg gccggcgagt tcatgatggg attgccgaat aatgagccgc
     8881 gggccggagc ctacgagcgg ccgccgcacc gcgttcgcat cgaccacccg ttttacatgg
     8941 gtaaatacga ggtgacacag gcccagtgga aacgagtgat gagcggtcag aacccgtcgc
     9001 acttccaagg cgatgacaac ctgccggtgg aaatggtcag ttggcatatg gctcatgact
     9061 tttgcgagcg tctcagccgc cgggttcgtc gttccgtgcg gttgcccagc gaggccgaat
     9121 gggaatatgc ctgcagagcg ggatcaacgt catattggtc tttcggagac gaggtgcagg
     9181 atcttggcga tcatgcatgg tatggggggc gtgacgtaga gggtagaacc caccctgtgg
     9241 gcatgaaaaa gccgaacgcc tggggcctgt atgacatgca cggtaacgtg cgcgaatggt
     9301 gcgccgactg gtttgagccc tatcctggga cgaaagcgcc gccgacggaa ttcgccggtc
     9361 cgcgtcagcg aatcctgcgt ggcgggtcat gggactgggt cgaaccggag aatcaggggt
     9421 ccgcccgacg ggatcgggca ttgccggacg cccggcgcga tcactatggc ttgcgcattg
     9481 tggtttccgc ggagggggca ccctctacgg cagggcccgt tgtcgtaacc actcgcggac
     9541 agtgacttgc gtgagagatc cctggtcaac aggtgggaaa cgggccacga gccgacaatg
     9601 caacgtcgcg ccccccgggc agccgacggc cgactcggag gctgcgccgg caaaggtgga
     9661 atgctccaaa acctgcaagc cctggttaga ccgtcagtcg tacgaacttt cgcttgccac
     9721 gttggagaat atcgccggtc ttgacagcaa gcgttccggt ggcctcttca accagctcgc
     9781 cgttgagacg aatgcctttt tcttcgatca gccggcgggc ttcgctgttg gtggcggcga
     9841 agccgcaggc cacgatgagc ttgagggtca tggcccggcc gtcctgcatg accgcgtcgg
     9901 ggacccggat ggtcggcgtg tcggccggta caaccagatc gcccgtcgag gccttcttct
     9961 ggtgaatctg ctcccattca gcccgggcct gctggacaag cttgcggtcg tgaaacgccg
    10021 aggccacctc cacagccagc cgtttcttgg cctccatggg atgggtcttg ctcgcgtcgg
    10081 tcaggtcgcg gatctcgccg tcgggtatgg aggtcagcaa cgtcagatag ttcgccatgc
    10141 agccgtcggg aagactcatc agcttgccga acatgtcgtg gggcgattcg gacacgccca
    10201 cgtagttgcc caatgacttg ctcatcttct cgacgccatc caagccgacc agaatcggca
    10261 tcaccatgac cacctgggct tcctggccgg cgccgcgctg caggtcgcga ccgacgaggt
    10321 tgttgaatgt ttgatctgtg ccgcccagtt ctacatctga ctggatgcaa acgctgtccc
    10381 aaccctgcat gagggggtag agcaattcgt gcacaccgat ggggatttcc gcctcgaagc
    10441 gctttcggaa atcctcccgc ttgagcatct ggccgacggt catctgcccg gccagcttga
    10501 ggacatccgc aaaggtcatc cccgcgagcc attcgctgtt tgggcggacc tccaatctgt
    10561 cgggcgaggt atcgagcacc ttgccagcct gctcgaagta ggtcttggcg ttggcctcga
    10621 tagccttgtc atccaggacc ggtcgtgtct tgctgcggcc ggaggggtcg ccgatcttgg
    10681 cggtataatc accgataatc agcaccgcct tgtgccccaa gtcctggaat tgccgcatct
    10741 tgcggaggac cacggtatgg ccgaggtgga tgtctggggc cgtcggatcc aatccgagct
    10801 tgatccgcag gggcgtatta gtctgccggg accgctgcag cttttgccgc aactcgtcca
    10861 aggtgtcaat ccgctcgacc ccacgggtca gcactctcaa ctgttcctca actgtcgcga
    10921 agctcatatt gtcattatcc ccgtaaactg cggccgatca acctcggacc ttctcccgat
    10981 gtccgggcgc acgatagtcc agggggttct cagaattccg atcgcttgcc ggggtcaagg
    11041 acagaggggc cggcgcgtga ggcgatcgct ccctgggggc tttcccgaga ggctatccgg
    11101 ccacgtatga tcgtccgcgc gagccccggc ggaggttccg caaggccgaa accggccccg
    11161 gcttacaaag cagcccttgt gggatatcat ggagtcgtgc aacgaggagc ggaggtcaga
    11221 cgtgagcagt gaaatctatg cccaagccgg agcaattccc tatcgtcacg actcgaacgg
    11281 ccggctcgag atcctcctga tccgtcggat cgacaaggaa agatggggaa tccccaaggg
    11341 gctgatcgat ccgggtgtct ctccgaggga aaccgcccgt gccgaagcca tcgaggaggc
    11401 cggcgtggca ggcgtgctct ccgagcaacc cgtcggcttc ttctcgttca acaagaagga
    11461 atgggacgcc atggttcacg tcacggtgtt cctgttgcgg gtgacggaga tccagcccga
    11521 gtacccggag cagtatcgcc gggagcgcga atggttcgcc ttcaacaccg ccgtcgaagt
    11581 cgccgggcga aagggcttgc gtgaactgct gctggacgtg cccaagctga ttaaggaaca
    11641 cctgccggag acacatgaat gaccgccggg ttctactttg cgggcgaggt cgcaggccac
    11701 ctttgtcgag cggcggcaaa ccagtcgcgc aaagccctct ctgcgggttt gccgcgcggg
    11761 gtgtagttgt agtcgtcctt tccgccgggc gtatcgttcc actcccacca caggacgccg
    11821 cccaattggt cgggcgtcag gcttttgccc ggctcatcgg agtacttcca ggtctccatg
    11881 aacgcgaggt aacaattgag ttgctcttcc agtccggcag gcgtggcttc ctgcttgtag
    11941 tagtagttcc acggttcgat ggacgcgcct tcctggctgc accatccggc ttcggtgaac
    12001 agcaacggct tgttgaccgt ggcctgccac ttaagcaggc ccttcttgag gggttcccac
    12061 gccttgatga ggttgtggag ggacggacga ggctcggacg aaagcttgta gtagctggtc
    12121 atcccgacga ggtccagttc gtcccagaac tcgatcactt tgtaatgatc ccagttagcc
    12181 gaataggaaa gcttgccgga gaactccctg cggacttcgc gaatcacctc gtaccagcgg
    12241 ttggtgaact tttcggtgct gaccagctcc gagcccacca tgaagacctc gaccttgccc
    12301 tcctcggcga tgcgggcgaa gtgcaggacg aatttgcgat actgctcgaa ccagtcgtcc
    12361 cagttgggcg ggctgatcac gcctcgccat tcatttccgc gcgggtcgga cagcaagatg
    12421 atcggcatga ggatgacccg caggccgttg tcgtgggcga tcttgaagat ttcgagccac
    12481 tgttccctgc tcggcgtcac gaccgggtcg atcttgaacg aatcggagcc ggcgtgctcc
    12541 tggtagccgg gattgctgat catgacggta tcggcgccga gatcggcaat ctctcgaagg
    12601 agcaccttgg cctgctggat tgctgtggcc gagtaatgta cctgccacga catgccatgg
    12661 taggggacgc ccgccaaggc cgagacgggc cttctcggaa gcgggggggt aacggccttg
    12721 ccgccccgat cagccgaccg gccggcggag tctcgcccgc cgtctccccg gcctgcacac
    12781 gtccaccaaa gtgccgccgc gaggacgcag agaccgcctg caacctgcca caccaccttc
    12841 atcccgttca gtctccctca acacatgcgc aagaagccgc tgtccactct gggcaaccca
    12901 acgtactcac atccgcgccg attgggcagc ccgcagatcg aactcgacct gcccgagttt
    12961 tccggcccga tgagccacct gtttcgcatc cacccagacc gtcaggcctg ctgccgacgc
    13021 ggcgtccgcg gaattgtaca cgatatccag ctcgtggccg cgatatcgca acctccgcaa
    13081 tctgaaccac ttcagcggtc cggggatcgg gtctattacc gcccggtcgc tgtttgacgg
    13141 ctgcaacccg accaggtgtc ggatgaccag atcgcagtag gtgctgtgga agtagtccgg
    13201 acagcccgtg ggatccggcg aaccgctctg gccatcgtag tattcggtga ccagcggccg
    13261 ggcgaggtcc ccattttcat actgcaagtg cgtatagcgg tccagagcgt gccagaagtg
    13321 ctccggtttg acgtgcggct gggtatagtc gcggagggcg atggccatga cgtccaagat
    13381 catacttgtg gcgtggggcc aagccgggcc gttccacatg cagccgtggg tcttgccccc
    13441 agccgccggc caagtggcca catggggggt ataggccggg cacttgcggg aaaccgtcgc
    13501 ggggggatag gccgtccaga actcggtcgg atcgaccagg taagccaggc tggccacgta
    13561 ttcggcctga ttggggaaca accggctcat gaacgggtaa aacccgacaa tttcgcggac
    13621 ccgagcgacg gccccgtcat gctcacgggc ggcatagcag aaccggtctg tcgggttcca
    13681 cagcttctcc aggcacgccg cccggatctt gcccgccagt tgctggaact cagcggcgcg
    13741 ggcttcgtcc tccaggaacc gccatgcttc cgcgagcgcg gtcgcgttgc cgtacaaata
    13801 ggcggcgtag tcccctcgct cgagcctggc atcgggcttg ctgttgtcgt aattctcgtt
    13861 gaagtagaag aacgacggct ggaactccat accggttgta ccgtgattgc gcggggtggg
    13921 tagaaagtcg ccgtcgcggt caaactgtcg aagcgtgccc cgcacgtcct ccgacatcgg
    13981 ctcgatcacc gactggagcc agcggcggtc gggatggact ttgaacgctt cccaggcggc
    14041 cttgacgatc cagttattgt agtcgccgcc cttttcgttg atccgtgcag agatgaagaa
    14101 cttgttggtg tcgtcaggat tgaggcagtg gttttgcacc tgcccgaacg cgtattgcgg
    14161 gtcacgaagc cagcgggtct cggcgatgat gtgcggtgag ctgaaggaga tcaggcgcgg
    14221 gaagtgaggc tggtgcgtcc cctcgaagaa gtacggataa ggcaggttgc ccgtgcgggc
    14281 ccggctcagg cagtgccggg cgacgaacca gcgataccag tacagcttgg tgatatatgg
    14341 atcgtcgcac tcgaagtgtg ggcagttgtc atcgaaccat ttctggtaga ccgccttgtg
    14401 gtgtggaagg gcattgggcc gactcgccca ttcgcgagcg gtttgcgcgg ccttggcggc
    14461 gtcttcctgg aacgacatga cgatgcggag actcaccgac cggccgggcc tgatgttgac
    14521 acgtcgccag agcgacgact gcttgctcgc aagtgtgaaa cggggcgcaa gcaggactgc
    14581 atgcccgcga accccgtgga agtcggccga gcctgtcagc gtgttgtccg ggccgcgctc
    14641 gcgggccaac ccgctggaga catgaagaag gcattcggcg gagaccttcg agtcgttcgt
    14701 gaccgtcacc aggtccacca gcgtatcgtc gtcggtgatg aacttgcgct cgatgaccac
    14761 gaagcggccg aaccgcgagt gagtcgcgag gtggctcggg aaccagtcga ctcggtccgt
    14821 tgccgaaacg aagggcttgt cttgtttgaa gatgttgtga ttggagctcg atatggcccg
    14881 gtcaagcggc tgaaacacga tcgggtcggc gatcttggcg taatggatgt aggagaactg
    14941 cccgcggaag ccgagtcccg gatccagtcg ttccgtgaac aaccgtttgc cggtgggtcc
    15001 gagggttccc tggcgggcat ccgggcggtg cagaagatca tcgatggcaa cgcgatccga
    15061 cttccagacg gcacagccgg caggcaacag gcaaagcagc aagcacatag gcgcccgacg
    15121 aatcatcatg ccgtggtctc cgcgcaagac agcgggccgg cagcccgagg actaccggcc
    15181 cgttggtgat accggcttac ttttcgagcc ccatggccca ggcgattccg ccgagcaggt
    15241 gcttctggta ggcggggttg gtccagacgt cttcgcgatg gccgagcgag gtgtagaaga
    15301 ccctgccctt gccttcggtc ctggtccagg cgatggggac aaggctgccg ggcttcatgc
    15361 ccatggcctc gaggtccgcc tcgggggtct tctgtgtgtc tacgctgatc agcaggcggg
    15421 agttctccgc agagaaggtg tcgccgaaca ggtagtactc atcctgcagt ttccagccct
    15481 gttcgagcat cttggtggcc gggtggtcgg gcacgtcgat cttcagcgtg gccaccctct
    15541 gttggtggtg ggtcttgaac gccgctccga tcatcttcac gtactccggc cagccgggga
    15601 tgcccttgtc ctcgccccgg aacgagtcgg tggctgaatg aatgccgata aacgccccgc
    15661 cgtcccggat ccacttcatc agcggctcac gactgatcag cgggtttccg gaagtgtaga
    15721 agacgatggc gtcgtactgc ttgcaggcgt ctccttcgag atcgtggaag tcctgcgaga
    15781 ggaacacctc gtatcccgcc ttggttgcga tctccttgaa caccttctcg gcatgggcca
    15841 tctcgccggt caacggccgg ttgaccaccg aatggcgaaa cccgaacgat tgcgaataga
    15901 acaggatctt cttttttttg gcgctctcgc cttgggccag acaaaccaac gccccgctca
    15961 cacacagcgc caagccgccg aacaacatcc aatgcagctt cttcatactc gtgtacctcg
    16021 caaactgtca ccccagatgt cctgaaagtg caaaggggac ccgccccctc gaccgggcaa
    16081 gcagcgtacc accggaacgg agagctttca aacccgcatc agagcggcgc tatgactgtt
    16141 tcttgaggag ccgagggttc atcctcttgg tcttcaaccg gcaggatgaa cctgccagct
    16201 ggtcaagccg gtgcggctct cacgcgatct ccacccacgt ccccgtgcgg gcgctgtcca
    16261 aaacggcctc gcaaaccttc tgggtttcga gggcgtcgcg gaaagtcggg gcggcggact
    16321 ggcccttggc ggcggcctca aggaagtcag ccgcctggtg gacaaagctg tgctcgtagc
    16381 cgatctggag gccgggcacc caccacttgt tcatataggg catgtcgccg tcggtaacgt
    16441 ggatgctccg ccagccgcga atgatcgact cgtcgcgatg gtcaaagtag ctcaggcggt
    16501 gcaggtcgtg caggtcccag gcgatcgagg ccttctcgcc gttgatttcg aaagtgtaga
    16561 gggccttgtg gccgcgggcg tatcgggtgg actcgaaaac cgcgagggag ccgttggcga
    16621 accgagccag gaatgcgcac gcgtcgtcga tgcccacggg ttcgacctta ccggtctggg
    16681 tgtgtttgcg ttctttgatg aacgtttctg tcattgcggt gacgctcttg atcggtccgt
    16741 tcagccagat ggcggtgtcg atgcagtggg cgagcaggtc gccggtcacg ccgctgccgg
    16801 ccgccttgac gtcgagccgc cacagcgcgt cgccgccctg cggcaggtcg gccgagatgg
    16861 tccagtcctg caggaagacc gcgcgataat ggaagatgcg gccaagccgg ccctggtcga
    16921 tcaggttctt ggccagcgtc acggccggaa cccgccggta gttgaacgag accaggttgg
    16981 gcacgccggc tttctcgacc gccgcgacca tttcctgccc ctcggccacg ttcagcgcca
    17041 ggggcttttc gcagatgatc atcttgccgg ccttggcggc ggcgatggcg atctccttgt
    17101 gcatgttgtt cggcacgcag atatcgacca gttcgatgtc cttgcggtcc aggagcttct
    17161 gccagtcggt ttcgactgac tcgtaacccc aggtgtcggc gaacgtcttg gccttgtcgg
    17221 cgttgacgtc gcagatcgcc ttgagcaccg gccggagcgg cgtccggaag aagttgtaga
    17281 ccttgcggta cgcgttcgag tgcgtccggc ccatgaaacc gcaaccgatg agtccgacgt
    17341 tgagaggttt agacatggtg tgtccctcaa gagcgtcaaa tccccaaaaa ccaggcaccg
    17401 gcagcaggtc agtttactta cgttcggcag gccgtgtaaa ggccacgatc gcaacgtgcg
    17461 cgttcttgat ccagatctct cccatttcgg cgtctggctc agtcaccgcc aggacagtcc
    17521 actcgggact cctccgtgca agtctgcccc gcagttcttc tgcaaatccg ccgcgttgcc
    17581 cggacaattg ttctttcagg attaccgtgc aatcctgccc gattctcgga gcccagtcgt
    17641 cggcgccctg cccagaatcc gcgcgagggt cattgcaccc acttatgcct gcactgctgc
    17701 agaacagaag caaacccaat gtgatcacgg cgaaacgaca catcatagag cacctcctga
    17761 acggatatga gagctgcata gcctcccgca aagcgctacg ttatcttcgg atgcgggacg
    17821 accacggcaa cttgttcagt acggttgcgt ttgccgttat cccacatgta ctcaggtgaa
    17881 gaataacgcc tgcctggcga ccaagcaagc gagacatcag ccttcactgg cttcaccggc
    17941 agcccgttcc tccatccgcc ggcggtgatc ggcctccagc ttctgccaga actcggacga
    18001 ccggcgctga tgctggtact gctgccgcac tggctcgttg aggtagcgct gaccggttat
    18061 gggcgagttc tgcaggctgt cggcccagcc gcggatctga cgcgatatcg actcaaccag
    18121 ggagttcaaa tctgagattt cagatttcaa atgcgtgaaa tgcggaagcc gctcggtcag
    18181 cagcagcatc gacctcactt cgccggccga gccccgcgcg atgtacagga aggtcagcaa
    18241 ttcctgcgtc gtcccccgct cgaagccctc cgcgatgttg ttccccaccg aaatcgccgc
    18301 ccgctgtagc tggttccgca ggtcgccttt gttggcaaaa gcccggtctt cagtcagggt
    18361 gaagacccgc acagccagtt ccatcgccgc ctgccaaacg gggaggtctt cgaaacgttg
    18421 gtaggtcatg gagacattct tctgtggcag gcgagtcaga caaacctggg tttccggcaa
    18481 tctgaaatct caaatctcag atctcagata gcccacggca gatctcactc ccttggggct
    18541 gcctctaccc attccaccca tgcgtatccc gcacggccgc catcgccgcc aggatggagt
    18601 tccaggtgtc gggcttcatc atggtggcgt tggggaacat gcagccgtcc cagcagatat
    18661 gcttgaactt gcgggtgggt tgacctttgt cgtccttcag ccagaggccg gcctcgcgga
    18721 cgatgtcgag cttgccgttg gggtcgttgg gcagacagtg gcggccggtc ttatcatgtg
    18781 aacccgatcc cttcaccgtc gcgtcgcttt gggcgacgtg gaaatcgatg gtccacgggc
    18841 gcagagccga ggtgagcgtc ttgtaggctt gcgtgaacgt ggtcgtgtcg ctccaggaga
    18901 agttctctgg cagaatgcgg tcttcgggag cgttcgtacc taggaggtaa tgcagggtat
    18961 gggccatatc cgcctggaag ccgagagtcc gtggctgatt cacgccttcc agcaccgcca
    19021 gcatggcctt ccaactgtgc atgccggccc agcaaacctc gccttcggcg gccagccgct
    19081 cgccgtggtc ctcggccacc ttgcaggctt ccttgaaggt gtcaatgatc ttcctggtgc
    19141 tgccggcggg gtcgtcgtac caggcatccg gtccgcacgc cgagtcg
//
LOCUS       DAOTPW010000102        18982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_2947_length_18982_cov_5.592962, whole genome shotgun sequence.
ACCESSION   DAOTPW010000102 DAOTPW010000000
VERSION     DAOTPW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18982
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2947_length_18982_cov_5.592962"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1470..1544)
                     /locus_tag="P5316_10825"
     tRNA            complement(1470..1544)
                     /locus_tag="P5316_10825"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1510..1512),aa:Asn,seq:gtt)
     gene            complement(2184..2600)
                     /locus_tag="P5316_10830"
     CDS             complement(2184..2600)
                     /locus_tag="P5316_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR85491.1"
                     /translation="MFSDYWALGLRLAQRRLALGGETLRRAGDRCSSLRTVHRRPSPG
                     TPATAKVAERIRKRRRRLFTFLDDPSLEAANNRAERALRPAVIARKSSCGNKTLRGKR
                     TWEILTSLAATCYQPSQDSVEYLRPFLALAPAPSAR"
     gene            complement(2950..4095)
                     /locus_tag="P5316_10835"
     CDS             complement(2950..4095)
                     /locus_tag="P5316_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85492.1"
                     /translation="MSSERASPRKRGAFTLIELLVVIAIIALLIAILLPSLSRAREIA
                     KRTSCAANLKAIAQACVIYSESNQHRFPTAWRPSNLVAGTAMHTNIGRYRGLPDGFIT
                     VTATTPSGTEDNGSNTRSYWKLLMGGRKAYLQPKQVICPSPRALGHKTSGPNPYPIVG
                     DPKEPNATAANLWRGSGPALQPGQESRWFDFDGAAVSNNSSECSDFSYSFQLSHEAAS
                     SAGRSYGVAMTNSQDPRRALAADRNPFSNLVRSQTRPTPVYGRYDYDQTAQATGMPPP
                     GDLDGNGTTTAAEWVQAINRKHKSLNSRNHSREGQNVSYVDGHAKWYNNPLCGADEDL
                     IWTPTSTDVNMKHVQLGNNSGTLEPWQANYMLTMSTAGVQTDSVLIP"
     gene            complement(4546..5934)
                     /locus_tag="P5316_10840"
     CDS             complement(4546..5934)
                     /locus_tag="P5316_10840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR85493.1"
                     /translation="MPRILIVEDEVGLARAMGDALTDAGHEVRLVHAGEKVMGEFRSF
                     QPQMLILDVRLGGISGLNLLTEIKTESPDVEAIVVTAYGSVEVAVDAMKRGAAEFLTK
                     PVDLDVLAVAVDRVWSASHARRRLEQYRSAQAEQLRQVQLVGNCPRIVAIREQVGRLA
                     ARAVSAGLDMPAILLTGETGTGKDLLAYNIHASLKHRTGPFVALNCSAVPAELFESEL
                     FGHARGAFSGATADKPGMFATADGGTLFLDEIADLPAALQPKLLRALETHTVRRIGET
                     RDRSIDICLIAATNRDIESLIREGRFREDLYYRLKVISFHLPPLRERGDDVLLLADLF
                     CSRLAAKYGIAGLNLTPAAREALRQHQWPGNVRELQHALEGAALSISGSTIDDADLPV
                     PVRADPITRAERNLDEGTPIDLEQLERSLIERALKQTGGNASAAARLLSIGREAMRYR
                     MSKFGLLPEGGE"
     gene            complement(5972..7414)
                     /locus_tag="P5316_10845"
     CDS             complement(5972..7414)
                     /locus_tag="P5316_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR85494.1"
                     /translation="MSLRYRLLLVYLIVVLLTAATVAVALLELRHSSRIIADLQHWHE
                     AVLRVERLRTAFEREALSPLGVSPVPPPEGLDFEKLLAETRNLLDFDLARWHLQVVAR
                     DYREWQELARRSATRPANATTPPVESAGSSPVEEDPLSAQTGVVRRTLQRTVWFLEGK
                     QTEVMNEANEQTSRTMAMLNVVLALLALHVLTVGWLFGRWVLVPMARLNRQVEALAHD
                     LPPDEPLLTAPPEMANLAGALDRARISLGEMRNRLVESERLTAVGQFAAQLAHNLRNP
                     LASIRALAQVVSRHDAGDGRIRDRMAEIIASVDRLNRWIGSLMEVVRQEATTLQMADV
                     MPVIHRVHEALSAELSSKDLTWRVDAPTGRVMCLHSPETLEHALVAMAVNAVEASPVG
                     GRITVRVEQTPEKACRISVTDDGPGLPRDNPEIIFDSSYSTKERGLGLGLSLMRLALA
                     RQGGATGAMNNPDGGAVVFIELPMSDVRSS"
     gene            7687..8172
                     /locus_tag="P5316_10850"
     CDS             7687..8172
                     /locus_tag="P5316_10850"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HRR85495.1"
                     /translation="MRQPSMKFNLIDRITELVPGRRIRAVKALTTAEEYLADHFPHFP
                     VMPGVLMIEAMTQAAAWLVRRTEDFAHSMILLAEARNVTYKSFVAPGQTLELTLDAKE
                     IGRTSSKFVGVGRRGDTEVVRAHLTLRHFNLAEDNPAMEASDRKLVAELRQRMEILTR
                     N"
     gene            8263..8652
                     /locus_tag="P5316_10855"
     CDS             8263..8652
                     /locus_tag="P5316_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRR85496.1"
                     /translation="MAMSRDEIFENVRNTLVEALAVEPDEVTEEATLQGDLGAESIDF
                     LDIVFRLEKGFGIKIPRGELFPDDLLNNPEFVSNGKFTPTGLAKIKAAMPHADFSGFD
                     ADPDVNKVPELFTVRTLVNYIQSKLAA"
     gene            8755..9240
                     /locus_tag="P5316_10860"
     CDS             8755..9240
                     /locus_tag="P5316_10860"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HRR85497.1"
                     /translation="MRWFWIDRFVEFESGKRAVAIKNLSLAEEHLHDHFPGFPVMPPS
                     LMIEGMAQTAGILVGEARNFSEKVILAKVRHAVFDGIARPGQQLRYEAVIESIADEAA
                     LTSGKIYCDGEAFGTVDLMFSHIDQNMSGLNFPEENFVFTEDFKNLLSTFQIKGPAGT
                     H"
     gene            9251..10588
                     /locus_tag="P5316_10865"
     CDS             9251..10588
                     /locus_tag="P5316_10865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRR85498.1"
                     /translation="MSSRRVVVTGIGLVTPIGIGTESVWNALLEGKCGIRRLTAFDPS
                     GFESQIGGEIDSLVMKNYVPRSYLKSTKVMARDIEIAVAAAYEAVKDAGLKTKCLIER
                     GEVQGPPNVDSTRLGANIGAGLICADLTELAGALATAVDESGRFSIRKWGSEGMNNLT
                     PLWLLKFLPNMLSCHVTIVHDAQAPSNTITCGEASSHLAIGEAFRTIARGAADVCICG
                     GAESKINPMGLMRQQLLKRLTTRHNDKPQQACRPFDVDRNGIVISEGGGLLILEELEH
                     ARRRGARVYCEVAGFGASNNTHSWYEPHPEGAGIALAIRKALDDANLKPDEIELVGAF
                     GSGAVAHDLSEARGIRAALNARGSAVPVLAIKGAIGNNGAGSGAIDLGIAALCISQGI
                     VPPALNADRVDPECGLNVVAGQPLKATIRNAVSTAYALSGGQNAALVLKRMEA"
     gene            10590..11906
                     /locus_tag="P5316_10870"
     CDS             10590..11906
                     /locus_tag="P5316_10870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRR85499.1"
                     /translation="MSRRRVVVTGIGWVTPLGDDIEIVWQRLLRGESAVRPTTLFDAS
                     TFPTTFSAEVRGFELGKYLGPDADKHKDASRNSRFALAAATMAWRDAGLAGNNSLDPT
                     RVGIYLGGGEGPIDFDHFVAAAVAGWDHQANGGRGNLDARKWVDRAVRELQAVSEREQ
                     DPNMAAGHIACQFNAQGPTFNTLTACAASTQAMGEATNLIRRGDADVIISGGTHSMLH
                     PFGLTGFNRLTALSTRNDDYQTASRPFDQTRDGFVLGEGSGILILEEYEHARRRGARI
                     YSEILGYGSTADAFRITDIHETGRGGIAAMRLALEDAGLKPGDIHYISAHGTGTEEND
                     KIESLAIASVFGGRETAPPVSSIKSMMGHLIAAAGAVELITCVLAIRDQVLPPTINYR
                     QPDPNCQLDYVPNQARKAKVVNTLSNSFGFGGQNDTLIAVACDREN"
     gene            12052..12702
                     /gene="eda"
                     /locus_tag="P5316_10875"
     CDS             12052..12702
                     /gene="eda"
                     /locus_tag="P5316_10875"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRR85500.1"
                     /translation="MKTATEIIREIETTGLVAIIRASGSDELVEVVNALRAGGVTCIE
                     VTMTTPNALEVIRAARKTVGDSAAIGVGTVLDSETARAAILAGAQFVVAPITDLSTIE
                     ICNRYSVPVMPGAFTPTEIVRAWQAGADFVKVFPTSTVGAEYIKDIRGPLPHIKLVPT
                     GGVNLDTVADFIKAGAAALGVGSSLVTKDAIKNRDFAGLTRTAAAFLERVKHARGG"
     gene            12715..13611
                     /locus_tag="P5316_10880"
     CDS             12715..13611
                     /locus_tag="P5316_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85501.1"
                     /translation="MANAVDVSPTRLRAAVLAAGFLFPMDRLRRPDDPSWPKAACWFV
                     PWGLLIGLVYLFIFRVTWRWFGEYQGIRWLPAVAVLAIDLGFCGHRLLAGIARLQHPA
                     KSGAVCGPAGMTLGVLLAVLLVLVAKYAMFLSLPVGNVVKATPSINWYLPGQVRWFCP
                     DMVIYRPLLLMPIWGRWAIGLAMLIGRVSTSQPGWLGRMTMGLSLPRVLAQWLVVTVV
                     TMVYCATVISGESVFIPSGRSLAYGLMISIAVMVIAYWASFLMARRGDGQTEPSILLT
                     GLVAELAFLALYLHLANLIYWF"
     gene            13756..15093
                     /locus_tag="P5316_10885"
     CDS             13756..15093
                     /locus_tag="P5316_10885"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85502.1"
                     /translation="MPVIRLRGARCRCARLTRRLNVLLSAGVMVCAPTSQTQGEQDLV
                     QAVRSRQVELQYRLMDASPDAEVELWYTRDRGASWQRWGIHKDHDRPITFEAPAEGLY
                     GFTLIVNDKGMSSSPPPTAGTEPQRRVFIDYTPPLVQWDSVEPVESFAARRIVQLRWT
                     AHDAHLSSRPVSLSYLCSIEQSWQVIEQELPNSGQYDWTLPAHVAGQITLKLAVRDLG
                     GHVVERLFGPVPIDRWVTKGIPLPETSTRPAGPTANTSTSKPAQPTTNPAGLRFLAKA
                     DRERVEQLWSRGSWHAGRNEYAVAAERFREALEIDPTHIPSLYDLGVIHYYQKEYAKA
                     IEKFQSVLALEPRHQLALRGIHESYIAQRQYAKSYEILQQLVRYNEKDARAWLDLGDV
                     LFKMERPGDAREMWTRALQADPAAAAVINDAKWRLSRYGAAVGGQAKTDRQQR"
     gene            15267..16283
                     /gene="holA"
                     /locus_tag="P5316_10890"
     CDS             15267..16283
                     /gene="holA"
                     /locus_tag="P5316_10890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRR85503.1"
                     /translation="MPAAQDKPSPANPVIYVVSGPEVFLRQEAIQGIVRRVLGSADRS
                     LSLSEYDGASTGLELADVLDDARTLPFLAEKRLVMVRDADLFITRYRAELEEYLENPS
                     PCGVLLLECKSFPATTRLYKRAQAAGEVIKCETIKPRMLPAWLADRARKTHGAQIDSR
                     AAALLQDLVGNDLGLLDGELEKLALYVGERKRIAVADVEALVGQHREEKVWDILSAMA
                     AGNEARAIGLWEEVWQTDRAAPGRAVAGIAFTVRRLLAAKRAQQTGTPISEIARMLMR
                     WNDEAGVRSELAAFSVQQLEQMLCTLLKADVDAKTGGPSVQSSIEAFIIEGCRNRRQK
                     RATG"
     gene            16344..17657
                     /locus_tag="P5316_10895"
     CDS             16344..17657
                     /locus_tag="P5316_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85504.1"
                     /translation="MKRARTLLLAAGVGGLLAAGCNNGQLQPLWPAWGQTGKHPEDAH
                     AVSQPTSAMPPASAPAEETEETSSAADAKMAAVSQRIEEYLTRVQQALTSAPQSRPAG
                     EPPRPSATRPTRDKTIEGTTPSPKPPALAVTSRPAGKANAAIAIDPQEHKSDGPPSPM
                     TAVMAAPARGPRVELVDVRPAIRPSQPTASAQSSAAANRPTTGEPSGPMPMDTAAMIA
                     YLEDVVARHPEQLDDQLKLRLLYLATGQDEKAVAPLNIGDPVRAEFVIALLKTLRNSK
                     KMIVDPASKDPSALAAVDELRRLLGQQLPVSIPRIALVTRINSFGDYLALNPPKFPAG
                     KPVQVFLYTEVNNFRSEPTDDGKLQTILSQKVEIFDSSGKIVWQRSEPNIVDRVVTPR
                     QDFFIPFPINLPDDLPAGEYILKVTIEDRLGATTDQQKLTFTVGQ"
     gene            17827..18606
                     /locus_tag="P5316_10900"
     CDS             17827..18606
                     /locus_tag="P5316_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR85505.1"
                     /translation="MSTYRIVILGSGTSHGIPMIGCSCPVCTSSDPRDKRTRTSVIVE
                     AAGCSFLIDTSPELRTQCLACNITRVDAVLYTHSHADHIVGLDDLRRFNALQKAPIPC
                     YGDAHTLHDLRRMFPYAFKDMPDYPSAKPHLEMIVINEPFDLFGVLVIPIPLYHGKLT
                     VLGYRVGPFAYCTDCNQVPEESMNLLQGLQVLILDGLRRRPHPTHFNLEQAVDVARQV
                     GAKQTFFTHIAHELGHEATNAELPAGMALAYDGQVIEIKDA"
     gene            18665..>18982
                     /locus_tag="P5316_10905"
     CDS             18665..>18982
                     /locus_tag="P5316_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85506.1"
                     /translation="MDSEEAVSGQQSAISSDVAHLPVDARCPHCGYSLRGLPENRCPE
                     CGRPFEVTELTQSFRPRWPQLLTWYLAACVIATILDLSPALLRWNAYLLLPHGTAAEP
                     LRDV"
BASE COUNT         3750 a         5896 c         5893 g         3443 t
ORIGIN      
        1 agcgttctac acgcggatca gcaccgacga agaccaccag aagtactcgc tcgatgccca
       61 aaaggaccgc ttggaggcgt tctgcaagag ccagtacgac gacgactggc aactcttcaa
      121 gctctaccgg gacactgagt ccgggacgca catgaaccgg cccggcctgg aggagatgct
      181 ctacgacgcc tcggtcaagg cgttcgacgt gctgctggtc tttcgcgtgg accggctttc
      241 ccgcaaggtc cgcgagttgg cccagatggt ggacgagctg accaagcaga gcatcgtcct
      301 caagagcatc acggagcctt tcgacaccgc caacgccgcc gggaagatga tgctccagat
      361 gctcggcgtc ttcgccgaat tcgagcacgc gaccatcgtc gagcgcacca aggtcggcat
      421 ggagaagaag gccaagggcg gcgacttcgt gggcggcatg gtgccctacg gataccggct
      481 cgaaccggag caggggcttg tcctccgcga ggaagaagcg ctcgtcgtga agaagatgtt
      541 ctcgatgtac gcgctcggca aggaaggcac ccagaccatc tgcaagaagc tcaacggttc
      601 cggctgccgc aggcgcagcg gcaagaagtg ggacaagcgg gtcatcatca acatcctgcg
      661 gaaccccgtc tacgtcggca aactccgctg gcgcgaagtc gtctatgaag gcaaccacga
      721 cggcatcgtg tccgaggtgc tcttcaacca ggcccaggaa attctggacc agcggcggga
      781 ggacgtgagc ggcaggcggt ggaacaacgg ctcgcggctg ctgtccggcc tgatgaagtg
      841 caagcggtgc ggacggccga tggtcggcat cagttgccac cggaagaagg acgccggcag
      901 catcccgtac tacgtgtgca ccaagcggct ctcgacgcac gactgcgacc aggactacgt
      961 tcgggcggac ctcctggagg cggccatcgt ccaggacatc aaggccatgt tccaggacga
     1021 ggacctcatg acgcgtatct gggcggaggc caaccggcgg ctcgactccg agaagccgga
     1081 cctcacgaag gacatccgcc gggtggaggc gcacatggcc gacgtgcggg cccgcatgga
     1141 gcgctatctg gaggccttcg agacgggcaa actcgatgcc gacacctgca acggaaaggt
     1201 ccgcgatctc aaagaacagc tggccgagtt ggagactgaa cggacggacc tcgaggagcg
     1261 gcgcgaacgg ctgagcctgc cggaactgga ccgcgagttc ctgcggaaga ccctggcgga
     1321 cttcgaggag gtcctggctt cggcggagaa cgcaaaaaag aagcacctcc ttcgccagtt
     1381 ggtgaagaag gtgctcgttc atagtcgcga tacggttgag gtctggtacg ggttacccgc
     1441 acccgaccca gctcgtatcg cgggaataat ggctccccga gtaggactcg aacctacaac
     1501 aacccggtta acagccgggt gctctaccat tgagctatcg gggaatcctg ggcttgctcg
     1561 cggcccagat cttcatttcg gattgccggc tgcgaggcgc gggagtggca gtttcgcccc
     1621 gatgcgcccg gccaagctcg gcactccata tacctaactt tattcgtttt cggcagtgag
     1681 tcaacccccg gatgcatccc gtgcaggggt aatcgaaggc caaggctgat tccagggcaa
     1741 gtacctgagc cgggcgaaac tggcggagat tggaggcgcg gcccaaaaga cgaactccaa
     1801 cgaggagatt gccgccggcc ggccggccgg ccgacgatcc gtgacagtct gggagctttt
     1861 tcggaacccc tacctgaaag agtaccggtg tcaagggttt tctctcggca gggtgcgcgt
     1921 ttttctccct tggtctgcga ccctcgttat gaacggcgag acgaggccgt ctgatccttt
     1981 tccaccgccg cggactgggg tgcgtcactt cgaggcacct gtcagccctg accacgctgg
     2041 ggcaagccgc acacgagctt ctgctcacgt tcttgtccga cggtcgccca ctcagaccgc
     2101 gcgccgtgag ccccaagcca tacctccgtg attattaccc cgcgttatcg cagggatcga
     2161 gaatcccttg ggacggcacg tgtttagcga gctgagggcg cgggagcaag ggccaagaat
     2221 ggccggaggt actcgacgga gtcctggctc ggttggtagc aggtggcagc cagactggtg
     2281 aggatctccc aggtgcgttt gccgcgcaag gtcttgttgc cgcaggacga tttgcgggcg
     2341 atgaccgcgg ggcgcagggc tcgctcggcc cgattgttgg cggcctccaa ggacgggtcg
     2401 tccaggaagg tgaacagccg gcggcgtcgc ttgcgaatgc gctcggccac cttggcggtt
     2461 gcgggcgtac cgggggaagg tcttcgatgt acggttcgca gcgatgaaca tcggtcaccg
     2521 gcccgccgca gcgtctcccc gcccaaggcc aaccgccttt gtgcaagccg caacccaagc
     2581 gcccagtaat ccgaaaacat gcgcaagccg cacaggtgtc tggccgtaag gtcttataac
     2641 ttcacggacc cggatcataa agcggtcaga ggtcgagggt ttcgcgcgtg cacttgacat
     2701 tcctgaaata tgcggccgca cggttgcgca gacgcgagaa acctgcggaa gtcgaaccgg
     2761 aggagtccga cgccgcccac cagccgggtc caaggccacg acgactgtgc tctcgaagtg
     2821 cgagagaggc gagatctttt tgcaggtgcg cacgacgaat aaaggaaaag cccgcggaag
     2881 cttatccgcg ggctttctcg cagtcagcta aaacaactcc aatgcagact ccgggcggtt
     2941 tgccgcaacc taggggatca atactgaatc ggtctgcacg cccgcagttg acatggtgag
     3001 catgtagttc gcctgccacg gctccagggt cccgctgtta ttgcccagtt gcacgtgctt
     3061 catgttcacg tccgtgctgg tgggcgtcca gatcaggtct tcatcggccc cacagagggg
     3121 gttattatac cacttcgcgt ggccgtccac atacgacacg ttctggcctt cgcggctgtg
     3181 attgcgggag ttgagcgact tgtgctttct gttaatggcc tgcacccatt cggcggcggt
     3241 ggtcgtacca tttccgtcaa ggtcaccggg tggcggcatg ccggtggcct gcgccgtctg
     3301 atcatagtcg tatctgccat acaccggggt tggccgcgtc tgagatcgaa ccaagttcga
     3361 gaacggattg cgatccgcgg ctagtgctct gcgcggatcc tgcgaattgg tcatagcaac
     3421 gccgtatgag cggccggccg agctcgccgc ttcgtggctc aactggaagg aatagctgaa
     3481 atcggagcat tcgctactgt tgttggagac cgcagcaccg tcaaagtcaa accatctgga
     3541 ctcctgtccg ggctgcagcg ccggaccgga accccgccac aagttggccg ctgtggcgtt
     3601 cggttccttc ggatcaccga caatcggata ggggttgggt cctgacgtct tgtgccccaa
     3661 ggcgcgaggg cttgggcaga tcacctgctt cggctgcaaa tatgctttcc tgccgcccat
     3721 cagaagcttc caataggagc gagtgttgct gccgttgtct tcggtcccgg aaggggtcgt
     3781 ggcggtaaca gtgataaagc catcgggcaa acctctgtaa cgtccgatat tcgtatgcat
     3841 ggcggtaccg gccacaagat tgcttggccg ccatgccgtc ggaaagcgat gctgattgga
     3901 ctcgctgtaa atgacgcatg cctgggcaat ggctttcagg ttggctgcgc aactggtgcg
     3961 cttggcgatt tcgcgagccc gcgacagcga cggcaacaga atggcgatca gcagcgcgat
     4021 aatcgctatc acgaccagaa gctcaataag tgtaaaggcc cctctcttgc ggggcgacgc
     4081 tcgttcactc gacatgacac gcctccttag gttccagaaa aagcctatca caaaacgtcg
     4141 accataaccg gatgccggcc gcctgccaag ccgccgtcag gacgccctgc ggggttcgcg
     4201 tgaggcatcc tcagcctgtc ggccccgacg tcgccacgcg ggtcgggggt ccgggctctg
     4261 ccggattgct ctacacgtcg cgtgccaaac gccaagtctc tttgcgagcc ttccaagagc
     4321 tgttccgccc ccctgttcac atatcgttgg ttcatttcaa ccaaccagtg gttgaaatga
     4381 accaattcgg gatggttcgc gtcaccatcg tgaagaagcc gtcatttgtg attctggagg
     4441 ataagagtca gtctggctta accatgcagg tctcggagag aatcagcgcg aggtgtttgc
     4501 ggaagaattc atccgaacaa atccctgatg ccggggggcg atcaactatt ctccgccttc
     4561 gggcagtaac ccgaacttcg acatacggta tcgcatcgcc tcgcggccga tcgacaacag
     4621 tcgagccgca gcagaggcat ttccaccggt ctgcttcagc gcgcgctcga tcaacgacct
     4681 ctcaagctgc tcaaggtcga taggggtacc ttcatccaag ttgcgctccg cccgcgtgat
     4741 cgggtccgcc ctgaccggca ccggcaggtc tgcgtcgtcg atggtcgagc ctgagatgct
     4801 cagggcggcc ccctccagtg catgctggag ttcgcgtacg ttcccgggcc attggtgttg
     4861 gcgcagcgcc tccctggccg ccggcgtcag attcaggccc gcgatcccgt acttggccgc
     4921 aagtcgggaa caaaacaggt ccgcgagcag cagaacgtcg tccccacgtt ctcgaagagg
     4981 cggcaaatgg aaggatatga ccttcagccg gtaataaaga tcctcgcgaa atctgccctc
     5041 cctgatcaac gactcaatgt cccgattggt ggccgcgatg agacagatgt cgatacttcg
     5101 atctcgcgtc tcgccgatcc gtctgaccgt gtgggtctcc aaggcgcgca ggagtttggg
     5161 ttgcagcgcc gcaggcagat cagcgatctc atccagaaaa agcgtgccgc cgtccgccgt
     5221 ggcgaacatg ccgggtttgt cggcggtcgc cccgctgaag gcgccccttg cgtgcccgaa
     5281 aagttccgat tcaaacagtt ccgcgggcac cgcgctgcaa ttgagggcaa cgaacggtcc
     5341 ggtacgatgc ttgagcgacg cgtggatgtt gtaagcaagc agatccttgc cggtgccagt
     5401 ctcacctgtg aggagaattg cgggcatgtc gaggccggcg gacacggctc gtgcggcgag
     5461 tctgcccacc tgctcgcgga tcgcgacgat tcgcgggcag ttgccgacca gttgaacctg
     5521 tctcaactgt tccgcctggg cgctgcgata ctgctcgagt cgtcggcggg cgtgcgacgc
     5581 cgaccataca cggtccacag ccaccgcaag gacgtcgaga tccaccggct tggtaagaaa
     5641 ctcggcggct ccgcgcttca tggcgtcgac cgccacttcc acgctcccgt aagcggttac
     5701 cacgattgcc tcaacgtcgg gcgactcagt cttgatctcc gtgaggaggt tcaacccgct
     5761 gataccgcca agccgcacat ccagaatgag catctgcggc tggaagctgc ggaactcgcc
     5821 catgaccttc tcgccggcat gtacgagccg gacctcatgc ccggcgtccg taagggcatc
     5881 gcccatcgct cgcgccaatc cgacttcatc ctcgacaatt aagatcctgg gcatggcaga
     5941 cctcggccga ttccagagcc tttcaaacct ctcaactgct tcgtacatcg ctcatcggca
     6001 gttcgatgaa cacaaccgcg ccgccgtcgg ggttgttcat ggcccccgtc gcgcccccct
     6061 gtctggcgag agccaggcgc atcaaggaca gccccaatcc taagcctcgc tccttggtgg
     6121 aataggacga gtcgaagatg atctccggat tgtctcgagg gagccccgga ccatcatctg
     6181 tgacggaaat ccggcacgcc ttttcgggag tctgctcgac cctaacagtg atacggccgc
     6241 cgaccggtga cgcttcgacc gcgttcacag ccatggcaac cagagcatgt tccagcgttt
     6301 ccggactgtg caagcacatg actctgccag tgggagcgtc gacccgccaa gtcaaatcct
     6361 tggaagaaag ctctgcgctc agggcttcat gcacacggtg aatgacgggc ataacgtcgg
     6421 ccatctgcaa ggtcgttgct tcctggcgga cgacttccat caggctgccg atccagcggt
     6481 tgagacggtc gactgaagcg atgatttccg ccatgcggtc gcgtatccgt ccgtcgcccg
     6541 catcatggcg cgacacaacc tgggccagcg cgcgaatgct ggccaagggg ttgcgaaggt
     6601 tgtgcgccaa ctgggcggca aactgcccaa ccgcagtcag gcgttcggac tcgacaaggc
     6661 gattacgcat ttcacccaag ctgattcggg cacgatccag agctccggcc agattggcca
     6721 tctcgggagg cgccgtcagc agcggctcgt ccgggggcaa atcatgagcg agcgcctcca
     6781 cctgtcggtt gagtctggcc atcggcacca gtacccaccg gccaaataac cacccgaccg
     6841 tcagaacgtg aagggccagc aaggccaaga caacattaag cattgccatg gtcctggaag
     6901 tctgttcatt tgcctcattc ataacctccg tctgcttgcc ctcgagaaac cagacggttc
     6961 gctggagtgt cctgcggacg acgccggtct gggcactcag cggatcctcc tcaaccggcg
     7021 acgaaccagc gctttcgacg ggcggtgttg ttgcattcgc cggtcgcgtg gccgagcggc
     7081 gggccagctc ctgccattcg cggtaatccc tcgccacgac ctgcaggtgc caacgggcca
     7141 ggtcgaagtc cagcaagttc cgcgtctcgg cgagcagctt ctcgaagtcc aagccctccg
     7201 ggggcggcac gggtgacacc ccaagcggag acaaggcctc cctttcgaaa gccgtgcgca
     7261 gtctctccac cctcagcact gcctcatgcc agtgttgcag atcggctatg atcctgcttg
     7321 aatgccgtag ctccagcaat gcgactgcca cagtagccgc agtcagcagc accacgatga
     7381 ggtaaaccag cagcaaccgg tatcgaaggc tcatgaatcg caccgaaccg gccattgggg
     7441 cccgaggcgc cccctggcag cccgcccacc aaacggtatc aggtgcccgt cgagggcaca
     7501 acaccccgcg aaccccggtc gtcttgtgct tgcaaaccgc gtgccagcgg tccgtcaggg
     7561 tcgcggcacg gcggtaagtc cataggctgt cgggaaatag tcgacgggac cggactttgg
     7621 gagggcttgc atcgaaacgg gagtgcgtct acactcctgt gctcgctgtg gataaacgat
     7681 ggggacgtgc gtcagccgag catgaaattc aacctgattg acagaataac ggaactggtt
     7741 cccggccgtc gtatccgggc ggtaaaggcc ctcaccaccg ccgaggagta cttggcagac
     7801 cattttcccc actttccggt catgcccggt gtcttgatga ttgaggcgat gactcaggca
     7861 gccgcttggc tagttcggag aacggaggac tttgcgcata gcatgattct cctggccgaa
     7921 gctcggaacg tcacctacaa gagctttgtg gcaccgggtc aaacccttga gctgacgctt
     7981 gacgccaagg agatcggccg gacgagcagc aagtttgtgg gggtcgggcg gcgcggcgac
     8041 acggaagtgg tccgggctca tctgacgctc cgccacttca acctggcgga ggacaacccg
     8101 gccatggagg ccagcgaccg gaagctggtg gccgagttgc gccaacgaat ggagatcttg
     8161 acaaggaatt gaccctattg aggccgttcg gggagatacg ggttgaggta aaccgatcct
     8221 ggcggcctgc gatgaagacc tagatgacgg aggaaacctg cgatggcgat gagccgggac
     8281 gagatcttcg agaacgtccg gaacacgctt gttgaagccc tggcggttga gccggatgaa
     8341 gtcaccgagg aagcgaccct tcagggtgat ctcggagccg agagcatcga cttcctggac
     8401 atcgtgttca gactggaaaa gggctttggc atcaagatcc cgcgcggaga gttgttcccg
     8461 gacgacctgt tgaacaaccc cgagttcgtg tccaacggca agttcacgcc caccggcctg
     8521 gcgaaaatca aggcggcgat gccacatgcc gatttctcgg gctttgacgc ggacccggat
     8581 gtcaacaagg ttcccgagtt gttcactgtc cggacgctgg tcaactacat tcagtccaag
     8641 ctggccgcgt gaccaacagg gtactcgccg atggaataga ccgacggccg gcccggcgcc
     8701 ggccgttgct gttggaaaca cggcggatca gccgctgcgg agcgacctga catcatgcgt
     8761 tggttctgga tcgacaggtt cgtggagttc gaatcgggaa agcgggcggt ggccatcaag
     8821 aatctctcgc tggccgagga acacctgcac gaccactttc ccggttttcc ggtgatgccg
     8881 ccgagcctca tgatcgaggg catggcccag acggccggca ttctggtcgg cgaggcccgc
     8941 aacttctctg aaaaggtgat cttggcgaag gtcaggcatg cggtcttcga cggcatcgcc
     9001 cggccgggtc aacagcttcg ctacgaagcg gtgatcgaat ccatcgcgga cgaggccgca
     9061 ctgacgtcgg gcaagatcta ctgcgacggc gaggcattcg ggacggtcga tttaatgttc
     9121 tcgcatatcg accagaacat gagcggcttg aacttccccg aggagaactt cgtattcacc
     9181 gaagacttca agaacctgct gagcacgttc cagatcaagg ggccggcggg cactcattga
     9241 ggattgaggc atgagttcac gtcgcgtcgt cgtcaccggg atcggattgg tcacgccgat
     9301 cggcatcggg acagagagtg tctggaacgc cctgctggaa ggcaagtgcg gcatccgccg
     9361 gttgacggct tttgaccctt ccggatttga atcccagatc ggcggcgaga ttgattcgct
     9421 ggtgatgaag aactacgtgc cgcgcagcta cctcaagagc accaaggtga tggcccgcga
     9481 catcgagatc gcggtggccg cggcctacga ggccgtcaaa gatgccggac tcaagaccaa
     9541 gtgtctgatt gaacgaggcg aggtgcaagg ccctccgaac gtggactcga cgcgcctggg
     9601 ggcgaacatc ggcgcgggct tgatctgcgc cgacctgacc gagctggccg gggctttggc
     9661 gacggcggtc gacgaaagcg gccggttcag catccgcaag tggggcagcg aaggcatgaa
     9721 caacctcacg cccttgtggc tgctcaagtt cctgcccaac atgctgagct gccacgtcac
     9781 catcgtgcac gacgcccagg ccccatcgaa cacaatcacc tgcggtgaag cgtcgagcca
     9841 cttggccatc ggcgaagcct tccggacgat cgctcgggga gcggctgacg tttgcatctg
     9901 cggcggcgcg gagagcaaga tcaacccgat gggcctgatg cggcagcaac tcctgaaacg
     9961 gctgacgacg agacacaacg acaagccgca gcaggcatgc cgcccgttcg acgtggaccg
    10021 caacggcatc gtcatcagtg aaggcggcgg cctgctcatt ctcgaggagc tcgagcacgc
    10081 acggcgacgc ggagcccgcg tctattgcga ggtggccggc ttcggagcaa gcaacaatac
    10141 gcacagttgg tatgagccgc atccggaagg cgccgggatc gccctggcga tcaggaaagc
    10201 cttggatgat gcaaatctga agcccgacga aatcgagctg gtgggagcat tcggttcagg
    10261 agccgtcgcc cacgacctgt ccgaagcgcg tggcattcga gcggccctca acgcccgcgg
    10321 atccgcggtt ccggttctgg ccatcaaggg tgccatcggc aacaacggtg cgggcagcgg
    10381 ggcgatcgat ctcggcatcg ccgcgctgtg catcagccaa ggcatcgtcc caccggccct
    10441 gaacgcagat cgcgtcgatc cggaatgcgg cctgaacgtg gttgccggac aaccgctgaa
    10501 ggcgacaatc aggaacgcgg tgagcacggc ttatgccctc agtggcggtc aaaacgcggc
    10561 cctggtgctc aagcggatgg aggcatgacg tgtcacgacg gcgagtggtg gtaacaggca
    10621 tcgggtgggt cacgccgctc ggcgatgata tcgagatcgt gtggcagcgg ttgctgcgcg
    10681 gcgaaagcgc cgtccggccg acgactctgt tcgacgccag caccttcccc acgaccttct
    10741 ccgcggaggt ccgcggcttt gagctgggca agtacctcgg tcccgacgca gacaagcaca
    10801 aagatgccag ccgcaacagc agattcgccc tggcagccgc gacaatggcc tggcgtgacg
    10861 cagggctggc gggcaacaac agcctcgacc ccacgcgagt cggcatctac ctgggcgggg
    10921 gcgaaggtcc gatcgatttc gatcatttcg tcgctgcggc cgtggccggc tgggaccacc
    10981 aggccaacgg gggccgaggc aatctcgacg cccgcaagtg ggtcgaccga gcggtgcggg
    11041 aattgcaggc cgtctccgag cgcgagcagg atccgaacat ggccgccggt catattgcct
    11101 gccagttcaa tgcccagggc ccgacgttca atacgctcac ggcctgtgcc gcgagcaccc
    11161 aggcaatggg cgaagccaca aacctgatcc gccgaggcga cgccgatgtc atcatttccg
    11221 gcggcacgca cagcatgttg cacccgttcg gcctgaccgg tttcaaccgc ctgaccgccc
    11281 tctcgacaag aaacgatgat tatcaaacgg ccagccggcc gttcgaccaa acccgcgacg
    11341 gattcgtgct cggcgaagga tcgggcatcc ttatcctcga ggagtacgaa cacgccaggc
    11401 gccgcggggc aagaatatac tctgagatcc tcggctacgg ctcgaccgcc gacgcgttcc
    11461 gcatcaccga catccacgag accggtcggg gcggtatcgc cgccatgcgg ttggctcttg
    11521 aggacgccgg tctgaagccg ggcgacattc actacatctc cgcacatggc accggcaccg
    11581 aggaaaacga caagattgag tcgctggcca tcgcttcggt tttcggcggg cgagagaccg
    11641 caccgccggt cagttcgatc aagagcatga tgggccacct gatcgcagcc gccggcgccg
    11701 tcgaactgat cacatgcgtt ctggcgattc gcgaccaggt gctgccgccg accatcaatt
    11761 accgccaacc ggaccccaac tgccagttgg actacgttcc caaccaggcc cgcaaggcca
    11821 aagtggtcaa cacgctcagt aacagtttcg ggttcggcgg ccagaacgat acgctgatcg
    11881 ccgtggcgtg cgatcgcgag aattgaccgg aagccgaaaa ggggcgcatg atcggggccg
    11941 ccaggggcgg cgcggacctg ccggcggaga aacacggcgg ggctcgcccg ccggagcccc
    12001 ctcggcgtcg gccggccggc ccggagtacc ggaccatacg gagtctcacc cgtgaagact
    12061 gctaccgaga tcattcgcga aatcgaaacc accggcctgg tggccatcat tcgggcatca
    12121 ggcagcgacg aactcgtcga ggtcgtcaat gccttgcggg ccggaggcgt cacctgcatc
    12181 gaagtcacga tgaccacgcc aaatgcgctg gaggtcatcc gcgccgcccg caagactgtg
    12241 ggcgactctg cggccatcgg tgtcgggacg gtgctggatt cggaaacggc ccgggccgcg
    12301 attctggccg gggcccaatt cgtggtcgcg ccgatcaccg atctatcgac catcgagatc
    12361 tgcaatcgct acagcgtgcc ggtcatgccg ggggcgttca cgccgacgga gatcgtccga
    12421 gcctggcagg ccggtgcgga tttcgtaaaa gtctttccca cgagcacggt cggcgcggaa
    12481 tacatcaaag acattcgcgg ccccctgccg cacatcaagc tcgttcccac aggtggggtg
    12541 aatctcgata ccgtggccga cttcatcaag gccggcgccg ctgcgctggg tgtgggatcg
    12601 tcgctagtga cgaaggacgc catcaagaac cgcgatttcg ccggtctgac cagaaccgca
    12661 gccgcgttcc tggagcgggt caagcacgct cgaggcggct gagcaagtag agccatggct
    12721 aatgcggtcg atgtatcgcc gactcgcctt cgcgccgccg ttctggcggc tgggttcctc
    12781 ttccccatgg atcggctccg ccgaccggac gacccctcgt ggcccaaagc cgcttgctgg
    12841 ttcgttcctt ggggattgct gatcggtctg gtctacctgt tcatcttccg cgtcacgtgg
    12901 agatggtttg gggagtatca agggattcgc tggcttccgg cggtggcggt gctggcgatc
    12961 gatctgggct tctgcgggca ccggttgctg gcgggcattg cacggctgca gcatccggcg
    13021 aaatcaggag ctgtgtgcgg tccggcagga atgacgctgg gcgtcctcct ggcggttctg
    13081 ctggtcctgg tcgcgaaata cgcgatgttc ctgtccctgc cggtgggaaa cgtggttaag
    13141 gctacgccaa gcatcaactg gtatctgccc ggccaggttc gctggttttg ccccgacatg
    13201 gttatctaca ggccgctgtt gctgatgccg atctggggac gctgggccat cggcttggct
    13261 atgctcatcg gccgtgtttc aacttcccag cccgggtggc ttggtcggat gacaatgggg
    13321 ctctcgctgc cacgggttct cgctcagtgg ctggtcgtca ccgtggtcac catggtctac
    13381 tgcgccacgg tcatatccgg cgagagcgtc ttcataccct cgggacgcag cttggcttac
    13441 ggcctgatga tctcgattgc agtgatggtc atcgcttact gggcaagctt cctcatggcc
    13501 cgccggggtg atggtcaaac tgagcccagc atcttgctta cgggcctggt cgccgagttg
    13561 gctttcctgg ccctctacct gcaccttgcc aaccttatct attggttcta aaggcactta
    13621 cggttatcgg ggaatcgtca catccgcccg atgccgctgg agtcatccgg gcatgtgttc
    13681 cgataaacct ttcagtgggc cccgacgcgg agcgttgggt tgtgccgctg ccagcggcga
    13741 aaagaggcgg gcaggatgcc agtgattcgt ctgcgcggag cgcggtgccg ttgcgcccgg
    13801 ctgacacgcc gtttgaacgt attgctgtcg gccggggtga tggtgtgcgc gccgacgtcg
    13861 cagacacagg gtgaacagga tcttgttcag gccgtccgat cgcgacaggt ggagctgcag
    13921 taccgcctga tggatgccag cccggacgcg gaggtcgagc tctggtacac ccgcgaccgc
    13981 ggcgccagtt ggcagcgatg gggcatacac aaggatcacg atcgcccgat caccttcgag
    14041 gcccccgctg aaggcttgta tggcttcacc ctgatcgtca acgacaaagg catgtcctca
    14101 tcccccccgc ccactgccgg cacggaacca cagcgtcgcg tcttcatcga ctacacgccg
    14161 ccgctggtcc agtgggacag tgtcgagccg gtcgagtctt tcgcggcgcg acggattgtc
    14221 cagctcagat ggacggctca tgatgcccat ctttccagtc gaccggtgtc gctttcgtat
    14281 ctttgttcga tcgagcaatc gtggcaggtc atcgaacagg aactgccaaa ctccgggcag
    14341 tacgactgga cgctgcccgc acacgtcgca ggccagatca cgctgaagct ggcggttcga
    14401 gatcttggtg gccacgtcgt cgaacggctt ttcggccctg ttccgatcga ccggtgggtg
    14461 accaagggca ttccactacc tgaaacgtcc acgcgtcctg ccggaccgac ggccaacaca
    14521 agtacgtcaa agccggccca accgaccacc aacccggccg gcctgcggtt cctggccaag
    14581 gccgaccggg agagagtcga gcagttgtgg tcgaggggca gttggcatgc cggccgaaac
    14641 gagtacgccg tcgcggcaga gcgattccgg gaggccctgg agatcgatcc gacacacatt
    14701 ccgtcgctct atgacctcgg ggtcatccac tattaccaga aggagtacgc caaggcgatc
    14761 gaaaagttcc aatccgtcct tgccctggaa ccccgacatc agttggcttt gcgaggaatc
    14821 cacgaatcgt acatcgcaca acggcaatat gccaagtcat acgagattct ccaacagctc
    14881 gtcagatata acgagaagga cgccagggca tggctggacc ttggcgacgt cctgtttaag
    14941 atggagcggc cgggcgatgc ccgggaaatg tggacaaggg ccttgcaggc ggatcccgcc
    15001 gccgcagcgg tgatcaatga cgccaaatgg cgtctctcac gatacggtgc cgcagtgggc
    15061 ggacaggcca agaccgaccg tcagcagcgc taggagcacg tgtgaaagcc gccgcgagtg
    15121 tacggtagga cagcaccggc gtcttcacgg tatctgaggc ttgccaaagg ttgagcttag
    15181 gcgccggctt tccgcacatg ttgtagcgcg gtcgaaaggc tcggtgactt gaagaaggcc
    15241 gccagaaaga cgcgagatag acgagcatgc cagccgccca agataagcca tcgccggcca
    15301 atccggtgat ctacgttgtt tccggtccgg aggtctttct cagacaggaa gcgattcagg
    15361 gaatcgttcg ccgggtcctg ggcagtgccg accggtcctt gtcgctgagc gagtacgacg
    15421 gagcctcgac cggactggag ctggctgacg tgcttgacga tgcccggacc ttgccgtttc
    15481 tggcggaaaa acggctggtg atggttcgag acgcggacct gttcatcacc cgctaccggg
    15541 ccgagttgga ggaatatctg gaaaacccca gtccttgtgg cgtgctgttg ctggagtgca
    15601 agtcgtttcc ggcaaccact cggctgtaca agcgggcgca ggccgcggga gaggtgatca
    15661 agtgtgagac aatcaagccc cgcatgttgc cggcttggct ggctgatcgg gcgagaaaaa
    15721 cacacggcgc tcagatcgac tcgcgagcgg ccgcgctgct gcaggatctg gtcggtaacg
    15781 atctgggatt gttggacggc gaattggaaa agctggccct gtacgtgggc gaacgcaaac
    15841 ggatcgcggt cgccgatgtg gaggccttgg tcgggcaaca tcgagaagag aaagtttggg
    15901 atatactctc ggcaatggcg gcgggaaatg aggcccgggc aatcggcctg tgggaggagg
    15961 tctggcagac cgaccgggcg gcccccggcc gtgcggtggc cggtatcgcc tttaccgttc
    16021 ggcgactgtt ggccgcgaag cgggcccaac aaaccggcac gcccatttcc gaaatcgccc
    16081 ggatgctgat gcgatggaac gatgaggccg gcgtgcggtc ggagctggcg gctttctcgg
    16141 tgcagcagct tgagcagatg ctgtgcacct tgctcaaggc cgacgtagac gccaaaaccg
    16201 gcggcccgag cgtgcagtct tcaatcgaag ccttcatcat tgaaggctgt cgcaacaggc
    16261 gacagaaaag agcgacggga tagatcggct ggtgcgatcg tcagggggca agcgccggcc
    16321 gagtcattcg ggagtcgagt gatatgaaac gagcacggac gctcctgttg gcagccgggg
    16381 tgggtggctt gctcgccgcc ggctgtaaca atggccaact tcaacctctg tggccggcgt
    16441 ggggccagac cggtaagcac cccgaggacg cccatgcggt ctcgcagccg acatccgcaa
    16501 tgccgcctgc gtcggctcct gccgaagaga cagaagagac atcctccgcc gccgatgcca
    16561 agatggccgc agtctcccaa cggatcgagg aatacctgac ccgcgtccaa caggccttga
    16621 caagcgcgcc ccaaagccgc cccgcaggcg agccgccacg accatcagcg accagaccca
    16681 caagagacaa gacgatcgag gggacaacgc cctcgcccaa gccgcctgct cttgccgtca
    16741 cgagtcggcc ggcgggaaag gcaaatgccg ccattgccat tgaccctcaa gaacataagt
    16801 ctgatgggcc accaagtccc atgacggccg tcatggccgc gcccgcccgt ggcccgcgcg
    16861 tcgagctggt cgacgttcga ccggcgattc ggccctcgca acccaccgcg tcagcccaat
    16921 catctgctgc cgctaaccga ccaacgacgg gcgaaccctc cggccccatg ccgatggaca
    16981 ccgccgcgat gattgcttat ctcgaggacg ttgtcgccag gcacccggag caactcgacg
    17041 atcagctcaa gcttcgtctg ctctacctgg cgaccggaca ggatgagaaa gccgtcgccc
    17101 ccctgaacat cggcgatccg gtgcgcgccg agttcgtgat cgccctgctc aagacgcttc
    17161 gcaactcgaa aaagatgatt gtggaccctg ccagcaaaga cccctcggcc ctggcggcgg
    17221 ttgacgaact ccgccggctt ctcggtcagc aattacccgt gtccattccc aggatcgcac
    17281 tggttacgcg gatcaacagc ttcggcgact acctcgccct gaacccgccc aagtttccgg
    17341 ccggcaagcc cgtgcaggtg tttctctaca ccgaggtgaa caacttccgt tccgagccca
    17401 ccgacgacgg gaaactgcag accatcttgt cgcaaaaggt cgaaatcttc gactcgagcg
    17461 ggaaaatcgt gtggcagcga tccgaaccga acattgtcga tcgcgtggtc actccccggc
    17521 aggacttttt catacccttc ccgatcaatc ttcccgacga tctgccggcc ggcgaataca
    17581 ttctcaaggt gaccattgag gacaggctcg gagcaaccac cgatcaacag aaactcacct
    17641 tcacggtggg acagtgagac ccaaggcgga aggacgaagg cggaaggatg agtcaagacc
    17701 ggctctattg ggcaactgag ttttcaatcc tcgtcgtcgc ccatccttca tccctcatcc
    17761 ttcatccttg agccttgagc cttcctcact ggcaagcctg ctgtttccgt gtagactacc
    17821 cgtccaatga gcacctaccg tatcgtgata ctgggcagcg ggacctcgca cggcattccg
    17881 atgatcggct gctcatgccc cgtctgcacg tcaagcgatc cccgcgataa gcggacgcga
    17941 accagcgtca tcgtcgaggc ggccgggtgc tcgtttctca tcgatacctc cccggagctg
    18001 aggacccaat gccttgcctg caacatcacc cgcgtggacg ccgtgctcta cacccactca
    18061 cacgccgatc acatcgtcgg ccttgacgat ctccggcggt tcaacgctct ccagaaagcg
    18121 cctatcccct gctacggcga cgctcacacc ctgcatgatc ttcgccgcat gttcccgtac
    18181 gcattcaaag acatgcccga ctaccccagc gccaagccgc acctggaaat gatcgtgatc
    18241 aacgagccgt ttgacctctt cggcgtcctc gtcatcccca ttcccctcta tcacggcaag
    18301 ctgactgtat tgggctaccg tgtcggacct ttcgcctatt gcaccgactg taaccaggtt
    18361 cccgaggagt cgatgaacct cctgcagggg ctgcaggtgc tcattctcga cgggctgcgc
    18421 cgccggccac acccgacgca tttcaatctc gagcaggccg tggacgtcgc tcgccaagtc
    18481 ggagccaaac agaccttctt cacccacatc gcccacgaac tggggcacga ggcgaccaac
    18541 gccgagctgc cggccggaat ggcactggcc tatgacgggc aggtgatcga gatcaaggat
    18601 gcatgacgat cgacgagccg gcggcttcat gccgccggtg aacccatgag gacagggaca
    18661 gactgtggat agcgaggaag cggtcagcgg tcagcagtca gcgatcagct cggacgttgc
    18721 tcatctcccg gtcgacgctc ggtgcccaca ctgcgggtat tcgcttcgcg gcctgcccga
    18781 gaaccggtgc ccggagtgtg gacggccgtt cgaggtgacg gagctcacgc agtcttttcg
    18841 accccgctgg ccgcaactgc tgacctggta cctcgcggcg tgcgtgatcg ccactatcct
    18901 tgacctctcg cccgcactgt tgcgatggaa cgcttatctt cttcttcccc acggcacggc
    18961 cgcggagccg ctcagagacg tc
//
LOCUS       DAOTPW010000103        18702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3014_length_18702_cov_5.362578, whole genome shotgun sequence.
ACCESSION   DAOTPW010000103 DAOTPW010000000
VERSION     DAOTPW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18702
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3014_length_18702_cov_5.362578"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="P5316_10910"
     CDS             complement(<1..1363)
                     /locus_tag="P5316_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85507.1"
                     /translation="MMSDECSMMNGERAGPGLHNASSLNAPAASKADSQGREPLGSVP
                     HRQVSLGGAAGAGGFIFIVFFLFLLAAVVPVPHVLANDSRSVGGEYRADVPYPEFMPM
                     WREGWSWTDENGEKIRYAHPDMPLGGYLFAIFRNSSDKMMVVEDVLLDGVSLAQAVAP
                     EHKPKNHPDDKYPSSLQFSRLPKDQIDRLVAAGEPVWWKTEPMIVPPGGYGQVTVRLR
                     RDPKVEVLTVSIPGLPETDARVAVPVKTKQPWFFSINFTPEFDAAYAYLRHPGGKGIA
                     PRQILLDGQDVTSRCTIVADDAIDTVPVIINPASAFKKGTWYLFQAVYADGGIARVSI
                     GAWQPGLVYGMWGYSKVGKTADDNRKFFLEDMRVHNINTLMYSIPGEVREFLRSKEGQ
                     EYSKKTGIRIMTNWVGDAVNPPFLFLTDEPDAGDFASRMLDPYKRIGSLAQWLVNRAN
                     MFRR"
     gene            complement(1353..2204)
                     /locus_tag="P5316_10915"
     CDS             complement(1353..2204)
                     /locus_tag="P5316_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRR85508.1"
                     /translation="MSLKYRFLAIDVDGTLMNSRNELPPAHRQALHRAHEAGMIVCLC
                     TGRMLAEARAVLDEIGLDSDVGVFVFGAIVSELSTGRTMHRTTIAHHLADRLVAHFRS
                     LGSPVLVLYDSAEAGVDYRYIPGERNRKYFERWLQISPSRTEHLAEWRPLPFEPVRIG
                     VIEDPAHIGETFTSVRQAFSPVELKCNSIFAPNYGLHVVECFAPTVNKWHGIMQVAGP
                     MGVDGSEVVAIGDDVNDLEMIRHAGLGVAMGNAVASVKAVARWHVPSNDEDGLAVAIE
                     RILKNDE"
     gene            2603..2923
                     /locus_tag="P5316_10920"
     CDS             2603..2923
                     /locus_tag="P5316_10920"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HRR85509.1"
                     /translation="MNDAKSFFSSLLDMSFSDFITPKIIKILFILAIIGSAIWALALL
                     AAGLGSGSAGGILGGLIGAPIAFILAVILSRVYLELLLVMFRIAENTGRLVEHAEGPP
                     KDAQ"
     gene            complement(2929..3588)
                     /locus_tag="P5316_10925"
     CDS             complement(2929..3588)
                     /locus_tag="P5316_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85510.1"
                     /translation="MSTLVPNRLLFRFEFPLRYRSSPVIDGDLSDWPDEFLLPDFGRL
                     DNCRSFGKVWMAWNETGLFIACRVEGRRAPFRCEPRRFWEGDNLRLCTDMRDTREIKR
                     ASRYCQQFYFLPAGGGRHGRDPCAGAARIHRAAENAPLPPEGSIPIAGRRDGDVYTLE
                     ARIPGEILSGFDPDEHPRIGLYTMLEDVELGQQYLTVGDEFNWHIDPSTWATAVLTRD
                     A"
     gene            complement(3798..8999)
                     /gene="uvrA"
                     /locus_tag="P5316_10930"
     CDS             complement(3798..8999)
                     /gene="uvrA"
                     /locus_tag="P5316_10930"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRR85511.1"
                     /translation="MSKRRRAMGKGAIPVADDRQRWLTVRGARHNNLKNVDVHFPLGR
                     FICVTGVSGSGKSSLVIDILRERLARDLNGAEEVSPGDCDGIDGLEHLDKVIDIDQQP
                     IGRTPRSNPATYIKVFDEIRDLYARLPDSKVRGYKPGRFSFNVPSGEEGGGRCEACEG
                     NGANRLDMEFLADIWVPCPVCEGRRFNRETLAIHFRGKSIADVLDMDVQEALRHFANI
                     PKIAGMLQTLHDVGLDYIKLGQSSTTLSGGEAQRIKLGRELVRRSTGRTLYILDEPTT
                     GLHFEDIRRLLQVLHGFVDSGNTVVVIEHNLDVIKTADWVIDLGPEGGEAGGYVVVAG
                     TPEQVAECEASYTGQALRPVLRLDGHPPQPPLAKGGGLQPPLAKGGRLQASLAKGGRL
                     QASLAKGGRLQASLADGGRLASGPGLAVPALGGEASAVPSLNGEERSLLPPLNKGGAG
                     GVAPGEGDNGEAAEVTVVGAGQHNLKDVTVSFPRGKITVCSGVSGSGKSSFALDTVYA
                     EGQRRYVESLSTYARQFLGQLAKPKVERIEGLSPAISIEQKTAARSPRSTVGTVTEVF
                     DYMRILWARIGIPYCPKCDVPIGTQTSDEIIDKVLDLPEGTRALVLAPIEQTGNEDYA
                     ALFAREQASGFARVRVDGQVYELEKPPSLDRKSRHSVELVVDRVVIRKRERSRIADSI
                     EMALSIGSGWVMLAPVDGGEALRFSQRRSCTQCGTAYEDLTPHHFSFNTRLGWCETCE
                     GLGTQKGASVAMVVPNPRRSIRDGAIAGWEDFEENTLLAEMVGLVADHLGVSPGIPWC
                     KLPAEAQRGLLYGTGAHWYDLGQGVRFQWKGFYPAIDEASRSSWQYRQRLSQLTSEVA
                     CQKCGGGRLRPDAAAVRVGGKTIVDVCRMPLDDCLRFFKDLRLDARQRRVAGELLFEI
                     RNRLQFLVDVGLDYVTLHRSAPTLSGGESQRIRLASQIGSGLTGVLYVLDEPTIGLHP
                     RDTRRLISALHRLRDLGNTLLMVEHDRDVITESDHVLDFGPGAGAFGGRIVAAGTPQQ
                     LRTAQESLTGRYLGGREAIPIPARRRPGNGRFLRIIGARQNNLRNIDVDIPLGCLVVV
                     TGVSGSGKSSLVNDVLHNALARRIHRAGVEPGAHDEIIGVEFIDKVINVDQSPIGSSP
                     TSNPATYTGVFDEIRALFARLPEAKVRGYNANRFSFNRPGGRCEACEGNGQRCIEMHF
                     LPDVWITCETCGGTRYKAETLEVRYKGKSIADVLSMRISEALEHFKNVPRVRRMLQTL
                     DDVGLGYLQLGQPAPTLSGGEAQRVKLAAELGRPSTGKTLYILDEPTTGLHFEDLRRL
                     LDVLHRLCDMGNTVVCIEHNLDVIKTADWIIDLGPEGGAAGGDIVAEGPPERVVASQR
                     SHTGRILAEVLRSGTRVERKIFDPARALVTEDVSITAEEDIGQAKMPWEIDGRRWHTR
                     DRVGPRGEPARWEGAALEWLIDQIEKIGQGRLSPTNYNSRSTVEIKMPGSASPWFFHA
                     RTGGVWLLDVTFRVPARAFSNAEVRRLIPLKTLDEMNELPIYGKEPRVNVRHSGRLTD
                     DVRILIHSKKEIATADCREFIKRAFQAYQRLIRKMGEDVEMRQPWKVNGRQWHLSQQM
                     LSKNTEKLWRGSLIVEMLGRLKKMVPEIREDWTRKVMVVLQHPEVPGVWARFITNQAN
                     AMRVEVHCARGQFTPALIDKLGIDPVIRHLSHHDQVQFWLQKSQQCDQEQLERLVRES
                     IASLRKE"
     gene            complement(8999..9571)
                     /locus_tag="P5316_10935"
     CDS             complement(8999..9571)
                     /locus_tag="P5316_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85512.1"
                     /translation="MANDDFAGLIRQVTTPLEEQGVVYAITGSVASSIHGEPRASIDV
                     DIVMNMRPNQVSRLVADLGPRFYADADVIRQACVDHSMTNVIHRKTSLKFDLSMLPDT
                     PYYREVLRRRVWVEYPESDLRICVVSAEDIVLMKLLWRKTTGSRKQWENALSVVRTQR
                     TRLDWTYLRGWAAELGIKTDLEELAREAGI"
     gene            complement(9561..9818)
                     /locus_tag="P5316_10940"
     CDS             complement(9561..9818)
                     /locus_tag="P5316_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85513.1"
                     /translation="MSGQSEVRIADEETCRRFAEVCRLTQERLGRMSAAERDRLLQEY
                     MAEVHTPEKKFEMLCRMTTTMLRLRRGAAQARQTEGSDDGK"
     gene            complement(9815..11728)
                     /gene="uvrA"
                     /locus_tag="P5316_10945"
     CDS             complement(9815..11728)
                     /gene="uvrA"
                     /locus_tag="P5316_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRR85514.1"
                     /translation="MGRPVISVRGAREHNLRNVSLDLPRNRLICFTGVSGSGKSSLAF
                     DTLYAEGQRRYIESLSAYARQFLGTLAKPDVDQITGLSPAIAIQQKTSGWNPRSTVGT
                     VTGIYDFLRVLYARLGRQHCPQCGDPITAQTSEAIVGRILSTQAGSRVLILAPVVRGQ
                     KGEHVELLEDLRRRGYVRARIDGRVVHLDRAPALGRHNRHDIEVVVDRLAVGHEVRAR
                     LAEAVENALALGNGTLIVAPAEPESSASAPSEAGGLLGELLLSSKYACAKCGTGFDTP
                     SPQLFSFNSPTGMCLTCDGMGAIYDFDPELLVPDPSLSFFDPCIAPMRKRPGRWRRHI
                     YEGVAKHVGFDIRLPWGRLPQRARDALLYGTGDEHITFEWRYRGGVWKHGGTFEGVIA
                     ELRSWYRKAKAGFACAYYEKYMSRKPCPDCRGARLNAQARHVLVGGKALHELCAMSVL
                     QVREFFDTLHLDPTERLIAAEVLKEVGGRLSFLSNVGLDYLTLDRPAPTLSGGEAQRI
                     RLAGQIGCGLVGVLYVLDEPSIGLHPRDNRRLLASLERLRDMGNTVVVVEHDEETMRT
                     ADLIVDFGPGPGVRGGEVVATGTIDEMAANEKSLTGAYLAGRRSIEVPKQRRPVDVET
                     MRKVTRGREDQKP"
     gene            12086..12289
                     /locus_tag="P5316_10950"
     CDS             12086..12289
                     /locus_tag="P5316_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85515.1"
                     /translation="MISMIRNRVLTALLVIASCLLPGAEIDCDCEDGEFEVEFDGFCD
                     DDCDHGCCGHSLDGFFFDFWWDD"
     gene            12675..15713
                     /locus_tag="P5316_10955"
     CDS             12675..15713
                     /locus_tag="P5316_10955"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRR85516.1"
                     /translation="MTLADELKILRPEFMAAPFWFWNDVMDPQEVRRQIAEMSRQNIG
                     GFFMHARMGRVTPYMSQEWMTAIKAAVDEARQRGMFAWIYDEDGWPSGFGGGAVNALG
                     EEYLQQYATAEIIPCNGSIGTKLERPKCIIAAYATGRKVNGFSDCQRLDPADLPGSWS
                     RLPPTCRERLVIFRRELHNYRRHFSPEAWADGYVDVLNPKVTRAFIQNIYEAYRREIG
                     KEFGRNVPGVFTDEPSYHELGWSEPVVRLPMSPVLEREFEKRYGAPLLDNLMTIAFGG
                     PDAIRARWCFYSCLARLFSRNYTRILAEWCARHKMVFTGHYLLEEHPRCATHVIGDPM
                     LHYYYQQYPGIDHLGKDLDLKDFWSSARVLVKQASSIAHQFDKPRVMCETFAGGGWRF
                     GIREQKWMADWQYAMGLNLVCQHAFHYSLRGFRKRDYPPSLSFQQPWWPFSKDLGAHF
                     ARLGYLLTRGKRVVNVLVMHPIESFFATHEPGGYPWPNDPMNEALKKLVECLLAYQID
                     FDFGNEVLLKQHGRVQGAGLKLGSGTYDAVIIPHSLTWRKSTVTMLRKFASSGGRLYY
                     VEPAPTHLEGEPTDAYGSLLDEAMSLGHWQAPEFRQCISDLVGPVAKRACRVLGNDPH
                     NRDIMVMHRRAEGQHIFFLASGAKTAHTSCVIFDVPGIPMLVNTVSGHLSPLPHTREN
                     DECRIELSFDFAKSHAILFKRGRRVPAPAKETQVFQKTLCCHGDQLPYQMDRDNPFIL
                     DAGELFIDGRSAGLMSTLDAEQALARHGNAPQAFMRFTATSELKVGGAELLLETPENF
                     EIRIDDRKIPVPAQPAWLIDPCLRRIPIPGGLKAGTNTIEIHFRWQPGLEIEPMYLLG
                     RFGTYVEGDKCRIGPLPKKLAVGSWHDQGLAFYAGIITYTLDVNADTRADRWELRCPE
                     FHSAVRVSVNGVDAGHILWAPYTLDITSHVRRGRNQISLHVANCLRNFFGPHHMGNED
                     EIDCLGPHNFFNTRHRVPEYRLKPAGLLGEVVLTGYRTDR"
     gene            15723..16529
                     /locus_tag="P5316_10960"
     CDS             15723..16529
                     /locus_tag="P5316_10960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85517.1"
                     /translation="MARRRRSDNQRYHDRVAGRYESIYDDAYWQWHDALTWGHLKRFI
                     PRDLISPVADLGCGSGKWGRRLLKSGYRVTFVDLSAKMLDEARRQVEEMGATAKAEFV
                     QADLADLSALPEGHFGLAMAMGEPIGLTESPQQAMRQIARCLASGGVLVATLDNRVPC
                     LDYYLERGDREGLEQFIRSGRTHWLTRDPSEQFEIHTFEPKSIERLVADAGLEMLDMI
                     GKTVLPMRRHRELIEDADERRRWARIEQRLAGDPANFGRCAHLQFAARKP"
     gene            16713..17228
                     /locus_tag="P5316_10965"
     CDS             16713..17228
                     /locus_tag="P5316_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR85518.1"
                     /translation="MELPDAEAVKLAARGDADAFMTLVERYRAPLIAYVHGKTRRRDE
                     AEDLAQEVFCKAWQHLPGLRDPAAFPGWLFRMAHNAILTAARQPRPAQLEDDPADSSP
                     AGNENSVEVHAAVAALSEEFRITVSLRHFSGMSTDEVARTLGIPPGTVRSRLSRAYAQ
                     LRRRLISRVEV"
     gene            17232..>18702
                     /locus_tag="P5316_10970"
     CDS             17232..>18702
                     /locus_tag="P5316_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85519.1"
                     /translation="MSSDLDRQLEQLLDQAGSDAQPRRPSWGEMRDRITAIPQVRGRR
                     LVLPRRLVAVVGVAAMIAIVLGIIFHLPLGPIAQAESPPIRVIQKDIELTVFNLFESD
                     RPTLFMPTSPPARGPVKMGMALVKDHRIVLNLRPGDNLVYFTDVAATIDPTSVRFWSE
                     TDPIATKVVEQNFEYDLATAGAILKRYLDRKITCVLKDGKQHEGFLCSHDGDIVLADK
                     PPSDSGEMRKTESISRDQIRAVQMPDVPKDLFVKPTLVWKIRTQRPGQHETTLMYACG
                     HAEWQADYVVAVTPGAPGEKDQLDLQGWVTIDNRTGSTYKDAGIKLIAGDVNRVPDPW
                     APDQVERFSMGIEFADLDAYALRLAPAKMPEKAFVEKSFFEYHLYTLSAPSTVKDQQI
                     KQLKLLKAEGVKAIRRYVFDSPPGTDESRRHVAVVLEFKNDKDNKLGMPLPKGRVRVM
                     QRDSDGDMQLVNTTEIDHTAKDEEMKLNIGSAFDVAAERA"
BASE COUNT         3657 a         5990 c         5593 g         3462 t
ORIGIN      
        1 cgcggcgaaa catgtttgcc cgattgacca gccactgggc gaggctgccg atccgcttgt
       61 atgggtcgag catcctgctt gcgaagtcac cggcgtccgg ctcatccgtc agaaaaagga
      121 acggggggtt gaccgcatcg ccgacccagt tggtcatgat ccggataccg gtcttcttcg
      181 agtattcctg cccttctttc gatcgaagga actcccgcac ctcgccgggt atcgagtaca
      241 taagcgtgtt gatgttgtgc acgcgcatgt cctcgagaaa gaacttgcga ttgtcgtccg
      301 cggtcttgcc gaccttggag tagccccaca tgccgtagac caggccgggc tgccaggcgc
      361 cgatgctgac tctggcgatg ccgccgtcgg catagacggc ctggaacagg taccaagtgc
      421 ccttcttgaa ggccgaggcg ggattgatga tcaccgggac ggtatcgatg gcatcgtcgg
      481 ctacgatcgt gcagcggctg gtgacatcct ggccgtcgag caggatctgt cgtggggcga
      541 tacccttgcc gcccgggtga cgcaggtagg cgtaggcggc atcaaactca ggtgtgaagt
      601 tgatgctgaa gaaccaaggc tgctttgttt tgacggggac ggcgacccgg gcgtcggtct
      661 caggaagacc aggaatcgag acggtcagca cctcgacctt cgggtcgcgg cgaagacgca
      721 cagtgacttg tccgtatccg ccggggggta cgatcatcgg ctcggtcttc caccataccg
      781 gctcgccggc cgctaccagc ctgtcgatct gatccttggg cagcctggaa aactgaaggc
      841 tggaggggta cttgtcgtcc gggtggttct tcggcttgtg ttccggggcg actgcctggg
      901 caaggctcac gccgtccagc agcacatcct cgaccaccat catcttgtcg ctgctattgc
      961 gaaaaatggc aaagaggtaa ccgccgagcg gcatgtccgg gtgggcatac cggatcttct
     1021 cgccgttttc gtcggtccat gaccacccct cgcgccacat gggcatgaac tcggggtagg
     1081 gcacgtcggc gcggtactcg ccaccgacag atcgggaatc gttcgcgaga acgtgcggca
     1141 ccgggacgac cgccgcgagg aggaaaagga agaacacgat gaatatgaaa cctccggcac
     1201 ctgccgcccc gccgaggctg acctgccgat ggggcacgga tcccaggggc tcgcgcccct
     1261 ggctgtctgc cttcgacgct gccggggcgt tgagggagga tgcgttatgt aagccaggtc
     1321 ccgcgcgttc cccattcatc atgctgcatt catcactcat cattcttcag tatcctctca
     1381 atcgccaccg ccaggccgtc ttcgtcgttg ctgggcacat gccaccgggc gacggctttc
     1441 accgaggcaa cggcgttgcc catcgccacc cccaggccgg catggcggat catctcgaga
     1501 tcattcacgt cgtctccaat agccaccact tcagaaccgt cgacacccat cggtccggca
     1561 acctgcatga tcccgtgcca cttgttgacc gtcggggcaa agcattcgac cacatgcagg
     1621 ccatagttgg gagcgaagat agaattgcat ttcagctcga ccggcgagaa agcctgccgc
     1681 acggaggtga aggtctcgcc gatgtgggcc ggatcctcga tgacgccgat tcgaaccggc
     1741 tcgaaaggca acggccgcca ctctgcaagg tgctcagtgc gagaaggcga gatctgcagc
     1801 caacgctcga agtacttgcg attccgctcg cccggtatgt agcggtaatc cacgccggcc
     1861 tcagcggaat cgtacagcac cagaaccgga gaacccagcg atcgaaaatg ggcgaccaac
     1921 cgatccgcca ggtggtgcgc aatcgtcgtg cggtgcatcg ttcggccggt ggacaactcg
     1981 ctgacgatcg ccccgaaaac gaacacgccc acatctgaat ccaggccgat ctcatcgagc
     2041 accgcgcggg cctcggccag catacgaccg gtgcacaggc agacgatcat gccggcctca
     2101 tgggcccggt gtaaggcttg gcggtgggcg ggtggcaatt cattgcgcga gttcatcaac
     2161 gtcccgtcaa cgtcgatggc gaggaaacgg tacttcagac tcatgacacg ggcactcaaa
     2221 ccacgcggct ggaaacccca aaacaggcag acaatgtgca tgcacggcga tgggttgtca
     2281 accgtggacc caccttgaag ccgagaagca cccgaaacgc gtcacgacgc gggggtatcg
     2341 cgcgaacggc aatcagcaag ccgatcgcga cagaaaagcc tgggcaagcg accggcggca
     2401 gaaagggcga ttccctgcga tcacgcaaga cccgaagccc cgcatcgata agacacgcga
     2461 aaagccggct ccggttgaca aaacagacca tcccctggga agatgatggc cgcacgccgc
     2521 tctccgcgtc atccgcgggg accgcgccgg caattcccgg gaaccgtgca ggcacatctg
     2581 cacatcacaa ggagatacgc caatgaatga tgccaagagt ttcttctcca gcctgctcga
     2641 catgtccttc agcgatttca ttacccccaa gatcatcaag attcttttca ttctggccat
     2701 tatcggctcc gcgatctggg cactggcgct tctggcggcc gggcttggat ccggatcggc
     2761 gggcggaatc ctcggtggcc tgatcggggc gcccatcgcg ttcatattgg cggtcattct
     2821 gagtcgagtc tacctggaac tgcttctcgt catgtttcgc atcgccgaga ataccgggcg
     2881 actcgtcgag cacgcagagg gcccgccgaa ggacgcccag tagcacggtc atgcgtctct
     2941 ggtcaggacc gccgttgccc aggtgctggg gtcgatgtgc caattgaact cgtcgccgac
     3001 ggtcaggtac tgctgtccga gttcgacgtc ctccagcatg gtatacagtc cgatccgagg
     3061 atgctcgtcg ggatcgaagc ccgagaggat ctcgcccgga atccgggctt cgagcgtgta
     3121 gacgtcgccg tcacgccgac cggcgatcgg gatcgagccc tccggcggca aaggggcatt
     3181 ctcggcggca cgatggatcc gggcggcgcc ggcgcacgga tcgcggccgt ggcgccctcc
     3241 gccggccggg agaaaataga actgctggca ataccgtgag gcccgcttga tctcgcgggt
     3301 gtcgcgcata tccgtacata ggcgaagatt gtcgccttcc cagaaccgtc gcggctcgca
     3361 tctgaaagga gcccttcggc cttcaacgcg acaggcgatg aacaggccgg tttcgttcca
     3421 cgccatccat accttgccga aagacctgca gttgtcgagc ctgccaaaat ccgggagcag
     3481 gaactcgtcg ggccaatcgg acagatcgcc gtcgatcacc ggcgacgagc ggtaccgcag
     3541 cgggaactca aagcggaaca acaggcgatt ggggaccagg gtgctcattt cacgtggccc
     3601 tcctcataag acggaagccg tgacggcaaa agcccaatga ccaaatccca atgaccaatg
     3661 aagctcgaag cccgaatgac gaaacctcag gtggagcatc gctgttcgga tttcgtgctt
     3721 ggtcattcat tgggcattca gatttcggca ttggtcattc cttggtcatt gggccttgag
     3781 cattggccat ttctgtgtca ttcctttctc agcgatgcga ttgactcgcg taccagccgc
     3841 tcgagctgtt cctggtcgca ctgctgcgat ttctgcagcc agaactggac ctgatcgtga
     3901 tggctgaggt ggcggatgac cgggtcgatc ccgagcttgt cgatcagggc aggcgtgaat
     3961 tgcccccgtg cgcaatggac ctcaacccgc atggcattgg cctggttagt aatgaaccgt
     4021 gcccagaccc cgggtacctc cggatgctgc agcaccacca tgaccttccg cgtccagtcc
     4081 tccctgattt cagggaccat tttcttgagc cggccgagca tctccacgat caaagatccc
     4141 ctccagagct tctcggtgtt cttcgacagc atctgctgcg acaggtgcca ctgccggccg
     4201 ttgaccttcc acggctggcg catctcgacg tcctcgccca ttttgcggat cagccgctgg
     4261 taagcctgaa acgccctctt gatgaactcg cgacagtcgg cggtggcgat ctccttcttg
     4321 gagtgaatca gaatgcgcac gtcgtcggtc agacgccctg aatggcgtac gttcacccgc
     4381 ggctccttgc cgtagatcgg cagctcgttc atctcatcga gcgtcttgag ggggatcagt
     4441 cggcgcactt cggcgttgct gaacgcccga gcgggcacgc ggaaggtgac atccagcaac
     4501 cacacgccgc cggttcgagc gtggaagaac cagggagacg cgctgccggg catcttgatc
     4561 tcgacggtgc tccgggagtt gtagttggtc ggcgacagcc ggccttggcc gatcttctca
     4621 atctgatcga tgagccattc gagggccgcc ccttcccacc tcgccggctc acctcgtggg
     4681 ccgacccgat ctcgcgtatg ccagcggcgg ccgtcgatct cccagggcat cttcgcctgg
     4741 ccgatgtcct cctcggcggt gatcgagaca tcttcggtaa ccagagcccg cgccgggtcg
     4801 aagatctttc gctcgacccg cgttccgctg cgaaggacct ccgccaggat acgcccggta
     4861 tgcgatcgct gactggcaac cactctctcc ggcgggccct cggccacgat atctccgcct
     4921 gcggcgccac cctccggacc aaggtcgatg atccagtcgg ccgtcttgat cacatcgaga
     4981 ttgtgctcga tgcagacgac ggtgttgccc atgtcgcaca accggtgcaa cacgtcaagc
     5041 agtcttcgca gatcctcgaa gtgcaggccg gtggtcggct catcgagaat gtaaagggtc
     5101 ttgccggtgc ttgggcggcc cagttcggcc gcgagtttca cccgctgggc ctcgccgccc
     5161 gacaacgtgg gcgccggctg tccgagctga agatatccga gaccgacgtc atcaagcgtc
     5221 tgaagcatcc gccgcacgcg cggcacgttc ttgaagtgtt cgagagcctc cgaaatacgc
     5281 atggaaagca catcagcgat gctcttgccc ttataccgca cctcgagcgt ctccgccttg
     5341 taacgcgtgc cgccgcaggt ctcgcaggtg atccatacat caggcaggaa gtgcatctcg
     5401 atgcagcgct ggccgttgcc ctcgcaggcc tcgcatcggc cacccggccg gttgaaactg
     5461 aaacggttgg cattgtagcc gcgcaccttg gcttccggca ggcgagcgaa cagcgcccga
     5521 atctcgtcga acacaccggt atacgtggcc gggttgctcg tgggcgagct gccgatcggc
     5581 gactgatcca cgttgatgac cttgtcgatg aattcaaccc cgataatctc gtcgtgggcc
     5641 ccgggctcca cgccggcccg atgaatgcgc ctggccaagg cgttgtgcaa tacgtcgttg
     5701 accagcgaac tcttgccgct gccggacacg ccggtgacga ccacgaggca accgagcgga
     5761 atgtcgacat caatgttgcg aagattgttc tggcgggcgc cgatgatgcg aaggaaccgg
     5821 ccgttgccgg ggcgtcgccg cgccgggatg gggatcgctt cgcggccgcc cagataccgg
     5881 ccggtgagcg attcctgggc cgttctgagc tgttgaggcg tgccggcggc cacgattcgc
     5941 ccgccgaacg ctccagcgcc cggcccgaaa tcgagaacgt ggtccgactc cgtgatcacg
     6001 tcgcgatcgt gttcgaccat cagaagtgtg ttgccaagat cccgaagccg gtgcaaggcg
     6061 gaaatcagcc gccgcgtgtc tcgcggatgc aggccgatgg ttggctcatc aagaacgtaa
     6121 agaacgccgg tgagcccgct gccgatctgg gaggcgaggc gaatccgctg agactcgcca
     6181 ccggagagcg tcggggccga acggtgaagc gtcacgtaat cgaggccgac atcgaccagg
     6241 aattgcaacc gattgcggat ctcaaacaga agttcgccgg ccactctgcg ctggcgggca
     6301 tccagccgca gatccttgaa gaaccgcagg caatcatcca acggcatccg gcagacgtcg
     6361 acaatggtct tgccgccgac gcgaaccgcc gcggcgtccg gccgcagccg ccctcctccg
     6421 cacttctgac aggccacctc ggaagtcagc tggctcagcc gctggcggta ttgccagctc
     6481 gaacgcgaag cctcatcaat cgccgggtag aaccccttcc actggaaacg gacgccttga
     6541 ccgagatcgt accagtgagc ccccgtgcca tagagcaggc cccgctgagc ttcggctgga
     6601 agcttgcacc agggaatacc cggcgagact cccagatgat ccgccaccag accgaccatt
     6661 tcggccaaca aggtattctc ctcgaaatcc tcccagccgg cgatcgcccc gtcacgtatc
     6721 gaacgtcgcg ggttgggcac gaccatcgca acgctggccc ccttctgcgt accgagcccc
     6781 tcgcaggtct cacaccagcc gaggcgcgtg ttgaaggaga aatgatgcgg cgtcaggtct
     6841 tcgtaggcgg taccgcattg cgtgcacgac cgtctctggg agaatcgaag cgcctcacct
     6901 ccgtccacgg gcgcgagcat gacccagccg ctcccgatcg acaaagccat ttcaatactg
     6961 tcggcaatgc gtgaccgctc ccgtttgcga atgaccaccc ggtccaccac cagctcaacc
     7021 gagtgacgac tcttgcgatc cagtgagggc ggcttttcaa gctcgtacac ctggccgtcc
     7081 acccgcacgc gggcaaaacc actggcttgc tcgcgggcaa acagcgccgc gtagtcttcg
     7141 ttgcccgtct gctcgatggg agccagcaca agcgctcgag tcccttccgg caagtccagg
     7201 actttatcga tgatttcgtc actggtttgc gtgccgatgg gaacatcgca cttggggcaa
     7261 taaggaatgc ctatacgcgc ccagagaatg cgcatgtaat cgaaaacctc ggtcaccgtg
     7321 cccaccgtgg aacgcggcga gcgggcggcg gtcttctgct cgatgctgat ggccggagac
     7381 aggccttcga tgcgctccac tttgggcttt gcgagctggc caagaaactg gcgagcatag
     7441 gtgctgagag attcgacgta tcggcgctgg ccttcggcgt acacggtgtc gagagcgaag
     7501 ctgctcttgc cgctgccgga cactccgctg caaacggtaa tcttgccgcg agggaaggaa
     7561 accgtgacat ccttgagatt atgctggccg gccccgacga ccgtaacctc ggcggcctcg
     7621 ccgttgtccc cttcgccggg ggctaccccc cctgcccccc ccttgttaag ggggggaaga
     7681 agagagcgct cctccccatt aagagaggga actgcactgg cttctccgcc aagagcagga
     7741 accgctaagc cgggcccgct tgccaacctc cccccgtcag caagggaggc ctgcaatctc
     7801 ccccccttag caagggaggc ctgcaacctc ccccccttag caagggaggc ctgcaacctc
     7861 ccccccttag caaggggggg ctgcaacccc ccccccttag caaggggggg ctgggggggg
     7921 tgaccgtcca gacggagcac cggccggagg gcctgaccgg tgtatgacgc ctcgcactcg
     7981 gcgacctgtt cgggcgtccc ggccacgacc acatacccgc cggcctcgcc gccctcgggg
     8041 ccaaggtcga tgacccagtc ggccgtcttg atcacgtcga gattgtgctc gatcaccacc
     8101 actgtgttgc cgctgtccac gaagccgtgg agcacctgca ggagtcgccg aatgtcctcg
     8161 aagtgtagtc ctgttgtcgg ctcatcgagg atatatagcg ttcggccggt actcctccgc
     8221 accagctcgc ggccgagttt gatccgttgt gcctcgccgc ccgacagcgt cgttgacgat
     8281 tgcccgagct tgatgtagtc caggccgacg tcatgcagcg tctggagcat gccggcaatc
     8341 ttcgggatgt tcgcgaagtg ccggagagct tcctgtacgt ccatgtccag gacgtcggcg
     8401 atgctcttgc cgcgaaagtg gatggcgagc gtctcgcggt tgaagcggcg cccttcgcag
     8461 acaggacagg gaacccagat atcggcgaga aactccatgt ccaggcggtt ggccccgttg
     8521 ccttcgcagg cctcgcaccg gccgccgccc tcttcgccgc tcggcacgtt gaagctgaac
     8581 cgccccggtt tatagccgcg cactttcgaa tccggcagcc gggcgtaaag gtcgcgaatt
     8641 tcgtcgaaca ccttgatata ggtggccggg ttgcttcgcg gagtacggcc gatcggctgc
     8701 tgatcgatgt cgatcacctt gtcgagatgc tcaagaccgt cgatgccgtc gcaatcaccg
     8761 ggactgacct cctcggctcc gttcaggtcg cgggccaggc gctcgcggag aatgtcaatg
     8821 accagcgaac tcttgccgct gcccgagacg ccggtcacac agatgaaccg tcccagcggg
     8881 aaatgaacat caacgttctt gaggttgttg tgcctcgccc cgcgcacggt cagccagcgc
     8941 tgccggtcat cagcaacagg tatcgcccct ttgcccatgg cacgccgtcg cttgctcact
     9001 agatccccgc ctcacgcgcc agttcttcaa ggtccgtctt gatgccgagt tccgctgccc
     9061 acccccgaag ataagtccag tccagccgag tacgttgagt cctgacaaca ctcagggcgt
     9121 tttcccactg cttcctggaa cccgtggtct tgcgccacaa gagcttcatg agaacgatgt
     9181 cttccgccga taccacgcag atcctgaggt cgctctccgg atattcaacc cagactcgtc
     9241 ttcgaagcac ctcccggtag tagggagtat ccggcagcat gctcagatca aatttgaggg
     9301 aagtcttacg gtggatcaca ttggtcatcg aatgatcgac gcacgcctgg cgtatcacat
     9361 ctgcgtcggc atagaaacga gggcccaggt cggcaacaag cctcgatacc tggttgggcc
     9421 tcatgttcat cacaatgtcc acatcgatgc tggcacgggg ttcaccatgt atgctgcttg
     9481 cgaccgagcc ggtgatcgca tagacaaccc cctgctcttc aagaggagtg gtgacctgcc
     9541 gaatcaagcc ggcgaaatca tcatttgcca tcgtcgctgc cctccgtctg ccttgcctgc
     9601 gccgccccgc gccgcaaacg aagcatcgtt gttgtcatcc ggcaaagcat ctcgaacttc
     9661 ttctccggag tatgcacttc cgccatgtac tcctgaagca agcggtcgcg ttcagcggct
     9721 gacatccggc caagccgttc ctgcgtcaac cggcacacct ccgcaaacct gcggcacgtt
     9781 tcctcatctg caatccgtac ttccgattgc ccgctcatgg tttctggtcc tctctgccac
     9841 gagtcacctt gcgcatcgtc tcgacatcca cgggccgtcg ctgcttcggc acctcgatac
     9901 tccgccggcc tgccaagtac gcgcccgtga gtgacttctc atttgccgcc atctcgtcaa
     9961 tcgtgcccgt ggccaccacc tccccgccgc gaacccccgg tcccgggccg aaatcaacga
    10021 tcaggtcggc ggttcgcatc gtctcctcgt catgctcgac gaccacgacg gtgttgccca
    10081 tgtcccgcaa tcgttcgagg ctggccagga gccgccggtt gtcgcgcgga tgcagtccga
    10141 tcgacggctc gtcaagaacg taaagcacgc ccaccaggcc gcagccgatc tgaccagcca
    10201 ggcgaatccg ctgggcctcg ccgcccgaaa gcgtcggcgc cgggcgatca agcgtcagat
    10261 agtcaagacc cacattcgac aagaacgaga gccgcccgcc cacctccttc aacacctcgg
    10321 cggcaatcag ccgctcggtc gggtcaaggt gcaacgtgtc gaagaattct cgcacctgga
    10381 gcaccgacat ggcgcacaat tcatgaaggg ccttcccgcc gaccaggaca tgccgcgcct
    10441 gggcattgag gcgggcgccc cggcagtcgg gacatggctt gcgactcatg tacttctcgt
    10501 aataggcgca agcaaaaccg gctttggcct tgcggtacca ggagcgcagc tcggcgatca
    10561 ccccttcgaa agtgccgcca tgtttccaca cgccgccgcg ataccgccac tcgaaggtga
    10621 tgtgctcgtc gccggtgccg tagagcaagg catcacgggc ccgttgtggt aggcggcccc
    10681 agggcagccg aatgtcgaac cccacgtgtt tggcgacccc ttcgtagatg tgccgccgcc
    10741 aacggccggg acgttttcgc atcggcgcaa tgcagggatc aaaaaaactc agggacgggt
    10801 ccggaaccag aagttccgga tcaaaatcgt agatcgcgcc catcccgtcg caggtcagac
    10861 acatgcccgt cggcgaattg aaactgaaca actggggcga cggcgtgtcg aaaccggttc
    10921 cgcacttggc gcaagcatac ttggaggaca acagcagctc cccaagcagg ccgccggctt
    10981 cgcttggcgc agatgccgac gactcgggct ccgccggagc cacaatgagt gtgccgttgc
    11041 ccagggcgag cgcgttttca acagcttccg cgaggcgagc ccgtacctcg tggccgactg
    11101 ccagtcggtc gacaacgacc tcgatgtcgt gccgattatg acgcccgagt gcaggtgccc
    11161 ggtccaggtg aaccaccctc ccgtcgatcc gcgctcggac atagcctcgc ctgcggagat
    11221 cctccagaag ctcaacgtgc tcgcctttct gccctcgcac caccggagca agaatcaaca
    11281 cgcgcgatcc ggcctgcgta ctcaggatgc gcccgacaat ggcctcggag gtctgggcgg
    11341 tgattgggtc tccgcattgg ggacagtgtt gacggccgag tctggcatac aagactctga
    11401 gaaaatcata gatgccggtc accgtcccca cggtgctgcg aggattccag cccgacgtct
    11461 tctgctggat ggcaatcgcc gggctcaacc cggtaatctg gtccacgtcc ggcttggcca
    11521 gggtccccag aaactggcgt gcgtaagcgc tgagcgattc gatgtagcgg cgctggccct
    11581 cggcgtacaa ggtgtcaaag gcaaggctgc ttttgccgct tccggagacc ccggtgaagc
    11641 agatcaaccg gtttcgcggc agatccaaag agacgttccg caggttatgc tcccgcgcac
    11701 cccgcacaga tatgaccggc cgacccatgt gcaaccccct gtctgccgct cgaccagaga
    11761 aactggccgg gcagaaaccg acccagcatt gtaattcagc gaagcatttt ggaccaaccg
    11821 acagtccctg cccaaaggac tgcggccgtg aggctcatgc cgaggcacag gattccgtaa
    11881 gtcgattcca gacaagccgt tatcgcctct ctgcaatatc aaataaggtg cctcaccggc
    11941 tgatgcgcca ggcagcctca gaaaacacgc gccaaacctt cgcggggaga atctgtctcg
    12001 taactatgta agaggagtcg ctctgagatc aggagtatcg tgtccgggtc ggaagccgac
    12061 ccgccaggaa cataaggagg cattcgtgat aagcatgatc cgaaatcgcg tgctgaccgc
    12121 cctgctcgtc atcgcgtcct gcttgctgcc gggagcagag atagactgcg actgcgaaga
    12181 cggggaattc gaagtggagt tcgacggctt ctgcgacgac gattgcgatc acggctgctg
    12241 cggccacagc ctcgacggtt tcttttttga cttctggtgg gatgattaaa gagacactca
    12301 acagcaatcg tcaggagacc ttggcttggt ttgagggttg tcaggcagat gcgcctgcca
    12361 tgaaacccaa caccagtgcg tgatacgttc tggctcgaaa cccccacttg cctaccacca
    12421 agctggaagt cgggcgggca tgaggcgaga ctgtgattct gcccgcccaa tcgcaacctc
    12481 cgaaactacc gtcctgtgag tcgtcgtccg ataatcacgc ccgccggcac gcggcgtctg
    12541 ctcgcaacca gtcacgcccg caaccctgtc gaacgtatcc aatttcgctg acacctactg
    12601 atatcccccg cagtttgtaa ttccggcccg attgcggtat cgtcggtgta cttgcgacca
    12661 caggagtgcc ccctttgacg ctggcggacg agttgaaaat actgcggcca gagttcatgg
    12721 ccgccccgtt ctggttctgg aatgacgtga tggaccccca agaggtccgg cgccagattg
    12781 ccgagatgtc gcgccagaac atcggcggtt tcttcatgca tgcccggatg ggccgcgtca
    12841 ccccctatat gtcccaggaa tggatgacgg caatcaaggc cgccgtggat gaggcccggc
    12901 aacggggcat gttcgcctgg atttacgacg aggatggctg gccgagcggc tttggcggcg
    12961 gtgcggtcaa tgcactgggc gaggagtacc tccagcagta cgccaccgcc gaaatcatcc
    13021 cctgcaacgg atccatcggc acaaaactgg agcgaccaaa atgcatcatc gccgcctacg
    13081 ccacgggcag aaaagtcaat ggcttcagcg attgccagcg gcttgatccc gcggacctgc
    13141 ccgggagctg gtcgcggttg ccgcccacct gccgcgagag acttgtgatc tttcgccggg
    13201 agttgcacaa ctaccgccgc catttctcgc ccgaggcgtg ggccgacggc tacgtggacg
    13261 tgctgaaccc caaggtaacc cgcgccttca tccaaaacat ctatgaggcc taccggcgcg
    13321 aaattggaaa agagttcggc aggaacgttc ccggcgtatt taccgatgaa ccctcatacc
    13381 atgaactcgg ttggagcgaa ccggtcgtac ggctgcccat gtcgccggtc ctggagaggg
    13441 agttcgagaa gcggtatggc gctccccttc tggataatct gatgaccatc gcgttcggcg
    13501 ggcccgacgc catccgcgcc cgatggtgct tctactcctg tctcgcgagg ctgttctcgc
    13561 ggaattacac ccggatcctc gccgaatggt gcgcgcgaca caagatggtc ttcaccggcc
    13621 actacctgct ggaagaacat ccccgttgcg cgacccacgt gatcggcgac ccgatgctgc
    13681 actactacta tcagcagtat cccggcatcg accatctggg caaagacctt gacttgaagg
    13741 acttttggtc gtcggcgagg gtgctcgtca aacaggcatc gagcatcgcc caccagttcg
    13801 acaagcctcg cgtgatgtgc gagaccttcg ccggcggggg atggcgcttc ggcattcgcg
    13861 aacagaaatg gatggccgac tggcagtacg ccatgggact gaacctggtc tgccagcatg
    13921 cttttcacta ttccctgcgg ggttttcgga aacgcgacta tccgccgagc ctttcgttcc
    13981 agcaaccttg gtggccgttc agcaaggatc ttggcgccca tttcgcccgg ctgggctact
    14041 tgctgacccg gggaaaacgg gtcgtaaacg tcctggtcat gcaccctatc gagagcttct
    14101 ttgcgaccca cgagcccggc gggtacccgt ggcccaatga cccaatgaat gaagccttga
    14161 aaaagctggt cgaatgcctg ctggcctacc agatcgactt tgattttgga aacgaggtcc
    14221 tcctgaaaca gcatggccgg gtccaaggcg ccggcctcaa actgggctct ggcacttatg
    14281 acgcagtcat catcccccac agcctgacgt ggcgcaagtc caccgtgaca atgctgcgga
    14341 agttcgcgtc ctccggaggg cgattgtact acgtggaacc cgccccgacg cacctggaag
    14401 gtgagcccac cgacgcctac gggtcgttgc tggacgaagc gatgagcctg ggccattggc
    14461 aggcaccgga attccggcag tgcatctccg atctggtcgg accggtggcc aaaagggcct
    14521 gtcgcgtgtt gggcaacgat ccgcacaacc gtgatatcat ggtcatgcac cggcgagccg
    14581 aaggacagca tatcttcttt ttggccagcg gggccaaaac cgctcacacg tcctgcgtca
    14641 tcttcgacgt acccggcata ccaatgctgg tcaacacagt gagcgggcat ctgtcgcctc
    14701 taccgcacac ccgcgagaac gacgagtgcc gcatcgagtt atcattcgat ttcgccaagt
    14761 ctcatgccat tctcttcaag cgcggccgtc gcgttcctgc acccgcaaag gaaacccagg
    14821 ttttccagaa gacgctctgt tgtcacggcg accagttgcc ttatcagatg gaccgcgata
    14881 accccttcat tctggacgca ggcgagttgt tcatcgacgg gcgttcggcg ggactcatgt
    14941 caacacttga tgccgagcag gctctggccc ggcacggcaa cgctccgcaa gcgttcatgc
    15001 gattcaccgc cacgagcgaa ctgaaagtcg gcggtgccga gttgcttctt gaaacgccgg
    15061 aaaacttcga aatcaggatt gacgaccgga agatcccggt gccggcgcaa ccggcctggc
    15121 tcattgaccc gtgcctgcgc aggatcccga ttcccggcgg tctcaaggcg ggcacaaaca
    15181 ccattgagat ccacttccgg tggcagccgg gcctggaaat cgagcccatg tatctgctcg
    15241 gccgattcgg tacctacgtg gaaggcgaca agtgtcggat tggcccgctg ccgaaaaaac
    15301 tggcagtcgg ctcgtggcac gatcagggtc tggccttcta tgcgggcatc atcacttaca
    15361 cactcgatgt caatgcagat actcgggcag atcgctggga actgcgatgc cctgagttcc
    15421 acagcgccgt ccgcgtgtct gtcaacggcg tcgatgccgg ccatatactg tgggccccct
    15481 acacgctcga tatcacctcc cacgtccgtc ggggccggaa ccagatcagc ctgcacgtgg
    15541 ccaactgcct gcggaacttt ttcggcccac accacatggg caacgaggac gagatcgact
    15601 gcctcggtcc gcacaatttc ttcaacacca ggcaccgcgt gcccgaatac cgcctcaagc
    15661 cggccggcct gctcggagag gtcgtcctta ccggctaccg cacggaccgc tagaataacc
    15721 gcatggctcg acggcgacgc tctgacaacc agcgctacca cgaccgcgtc gcgggacgat
    15781 acgagtccat ctacgacgac gcgtactggc aatggcacga cgccttgacg tgggggcacc
    15841 tcaagcgatt cattcccaga gatctcatct ctcccgtcgc cgacctgggt tgcggctcgg
    15901 gcaaatgggg ccgacgtctt ctcaagagcg gctaccgggt cacgtttgtc gatctgtcgg
    15961 ccaagatgct cgatgaggcc cggcgacagg ttgaagaaat gggggcgacc gccaaggcgg
    16021 agtttgtcca ggccgatctg gcagacctgt cggccctgcc cgagggacat tttgggttag
    16081 cgatggctat gggcgaaccg atcggactga ccgagtcgcc tcagcaggcc atgagacaga
    16141 tcgcccgctg tctggcaagc ggcggcgtgc tggttgccac gctcgacaac cgcgtgccct
    16201 gcttggatta ctaccttgaa cgaggcgacc gcgagggatt ggagcagttc attcgcagcg
    16261 gacgcactca ctggttgact cgcgatccct cagagcaatt cgagattcac accttcgaac
    16321 ccaaaagcat cgagcgactg gtcgccgatg cgggtctgga gatgctggac atgatcggca
    16381 agacggtgct gccgatgaga cgccaccggg agctgatcga ggatgcggac gagcgacgcc
    16441 gttgggccag gatcgaacag cgtctggccg gcgacccggc caatttcggc cgttgcgcac
    16501 atctgcagtt cgcggcgcgc aagccctgac gtaaccaagc ggtccgccat agaagcctcg
    16561 ccccaggtct accggtccca cctcgttgca tccgctcacc gcgcgggtac catgacttgg
    16621 caggtgcttt tcaagaaaac tcatccctca gcggttttcc gggagcagaa tcgcccgcga
    16681 cgcgtctaac gttgcaggaa caggggcgaa ccgtggaatt gcccgacgcg gaagcggtca
    16741 agctggcggc caggggtgat gccgacgcct tcatgacgtt ggtcgaacga taccgcgcgc
    16801 cactgatcgc ctatgtccac ggcaagaccc ggcggcgcga tgaggccgag gatctagccc
    16861 aggaggtctt ctgcaaggca tggcagcacc tgcccgggct gagggacccg gcggcgtttc
    16921 cggggtggct gttcaggatg gcccacaacg cgatcctgac ggcggcccgg cagccacggc
    16981 cggcacagct tgaagacgat cctgcggaca gcagccctgc cggcaatgag aacagcgtcg
    17041 aggttcacgc ggccgtcgcg gctttgtcgg aggagttccg gatcacggtt tcgctccggc
    17101 atttttcggg gatgagtacg gatgaggtgg ccagaacact gggaattccg cctgggaccg
    17161 tgcgaagccg attgtcgcgg gcgtatgctc agcttcgccg gcggctcatc tcgcgtgtgg
    17221 aggtctgatc ggtgtccagc gacctggatc gacaactcga acagcttctg gaccaggccg
    17281 ggtctgacgc ccagcctcgc cggccctcct ggggcgagat gcgagaccgg atcacggcca
    17341 tcccgcaggt ccgcggtcgg cgactggtcc tgccccgccg gttggtggct gtcgtggggg
    17401 tggcggccat gatcgcgatt gtcctcggca tcatcttcca cctgcccctc ggccccatcg
    17461 cccaagccga aagcccgccg attcgcgtga tccagaagga catcgagctc acggtattca
    17521 acctgttcga gagcgatcgc ccgacgttgt tcatgccgac gtcgccgcct gcacgcggac
    17581 cggtcaaaat gggcatggcc ctggtcaagg accaccgcat cgtcctgaac cttcggccgg
    17641 gggacaatct ggtctacttc accgatgtcg ccgctaccat cgacccgacc agcgtgcggt
    17701 tctggagcga aaccgacccc atcgccacga aggtcgtcga gcagaacttc gaatacgacc
    17761 tggcgaccgc cggggcgatc ctcaagcgct atctcgaccg caagatcacc tgcgtactga
    17821 aggatggcaa gcagcatgag ggcttcctct gctcgcacga cggcgacatt gtgctggccg
    17881 ataagccgcc gtcggactcg ggcgagatgc gcaagaccga gtcgatctcc cgcgatcaga
    17941 tccgggcggt gcagatgccg gatgttccca aggacctgtt cgtcaagccc accctggtct
    18001 ggaagatccg cacacagcgt cccggccagc acgagacgac gctcatgtac gcctgcggcc
    18061 atgccgagtg gcaggcggat tacgtggttg cggtcacacc cggcgctccc ggagagaagg
    18121 atcagctcga cctgcagggc tgggtgacca tcgataaccg cacaggatca acctacaagg
    18181 acgccggtat caagctcatc gccggcgacg tcaaccgggt gccggatccg tgggcaccgg
    18241 atcaggttga gcggttctca atgggcatcg aattcgcgga tttggacgcg tatgcgttga
    18301 gactggcacc ggccaagatg ccggagaagg cgttcgtcga gaagtccttc ttcgaatacc
    18361 acttgtatac gctctcggcc cccagcacgg tcaaggacca gcagatcaag caactcaagc
    18421 tgctcaaggc tgagggggtg aaggcaatcc ggcgatatgt cttcgattcg ccgccgggca
    18481 cggatgagag ccggcggcat gtcgccgtag ttctggagtt caagaacgat aaggacaaca
    18541 agctgggcat gccgctgccc aaggggcggg tccgtgtcat gcagcgggat tcggacggcg
    18601 acatgcagtt agtcaacacc accgagatcg accataccgc caaggatgag gagatgaagc
    18661 tcaatatcgg ctcggcgttc gacgtagccg ccgagcgggc gg
//
LOCUS       DAOTPW010000104        18629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3026_length_18629_cov_5.636266, whole genome shotgun sequence.
ACCESSION   DAOTPW010000104 DAOTPW010000000
VERSION     DAOTPW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18629
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_18629_cov_5.636266"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="P5316_10975"
     CDS             complement(<1..923)
                     /locus_tag="P5316_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRR85520.1"
                     /translation="MTLAAAWLVQASPAASAVQVNAGLNPETIYFGTQTILTVSVEDD
                     TGEWPSVEAVEGVHITRYGSPSVIHDLISGRVQRSYRFLVNPTRPGTFQIPAVAVKVG
                     EETVTRGPFTLRVKEAPIRCIAAELNPKETLVGRPATLILAFQGHRPGARPVLPSIEG
                     LTITSTGDPQIQQDNQGVPVRVFRYHVTASKLGTFQIRGISFAGVSADEVTLTVSPFV
                     IVGTRVSSQSLVIGEEATLQLDTLGLSAESEPTPVAPPGLEIKSVGEPAHGVTGTTTF
                     SFVVRGVEPGNPVITTLKLGDGRQVPLTQPIS"
     gene            complement(1151..>3366)
                     /locus_tag="P5316_10980"
     CDS             complement(1151..>3366)
                     /locus_tag="P5316_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85521.1"
                     /translation="GGTLPGRRPQKGFWGQRRGFWGQRRGFWGQWLLVMLWLIAVLPV
                     VARADIRALLLDAEEQFEQNRFEQALKLYKQADEAEPDHPAVWYNMGLCHMELGDGDK
                     AIQQFEKTASHSGVPAYLKQDAFYNVGLARAASARQKLNDLMAPATQPTDRKPAPDDP
                     ANIENLGKIAEELLQAITAFRQARDVGPDDDVEHNIRAARIIRRNVLGLLRRATEAKE
                     KEDILKDPRAYLESLIAEQDQQVSLARYLVIKPPEDAAAVRHARRTAIRGQRKLMERT
                     GELADQLAQYRQQQADAGGAATSQASTQPVEKTPLEKAYHAASEQLRKAVDSQKDACA
                     FLLDGEIKPGWDKQFAALEQMYGALFLYPLEPAPALAKARAIQQVLRGLVAEVKKETD
                     WLSDPLLGDVEWPKDAKWDADKTSIFVQQAVVGGTLTLLQNQFRILSTRPADEAQVPQ
                     EQQGNPMLDRELNEKLSKILEPAPETGQRCLKAITERSRKDTLAAQDELLRMIDEAME
                     LLPRTIEQRLQELIVKQAELNSQVKVEAGDQPVDESKKPSPLDEVRKWAAELKTRLLA
                     PRASKVADGFRERQEKIQKDCGKAAAQIRDEIPAEAASQPAGAPPASQPSRVQALIEA
                     GKHVGAADEQMTQAIEGLNKASVENSRKPLKSGGPVQAPQAKALEELIKALMVLQPPQ
                     SQPQDDQRDQEDRQQQEQQRDRQDIQRALEQAEKQRRGVERQLHQRRPRTVIKDW"
     assembly_gap    3367..3466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3880..5001)
                     /locus_tag="P5316_10985"
     CDS             complement(3880..5001)
                     /locus_tag="P5316_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRR85522.1"
                     /translation="MGAEFFNLNHPEALQWLWALVPPAVLFGFDLRRRQRAVSLFVSK
                     SLLDDVCPRRSIGRPIVRFAVLCVGLACLILAAAQPRWDPRQIEMPQQGQNLLFCLDV
                     SNSMRARDVDPSRLEAAKAAIRSLIGQLPAGNQVGLMAYAGTSELKCPLTPNYSHLLW
                     VLQDVTFNSVDEGGTNLGDAIYSAIRNVFGLDPTQAVDDKKKASATQPSTKLKVEEPD
                     SHRNANVLVILTDGESHEGDAREMAALAHRLGVGIYIIGLGTAAGAPIPIEVDGKITT
                     LKYKDQEVITKFDDASLRSIVADLPDRCGYLAAGASNVDLTDIYKNVIAKQGMQTKDV
                     RITVWQEKFQLFVGIGVVLVVLSTLISEQRPVARAGVGR"
     gene            complement(5002..6249)
                     /locus_tag="P5316_10990"
     CDS             complement(5002..6249)
                     /locus_tag="P5316_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRR85523.1"
                     /translation="MRFIEFANPHALWLVLLVPLYLFVLRRRPVTAALRYPSVRNLKR
                     LPKSLRQRCRILLPVLRALALLLLIVALARPVRKLETQELPSQGLAIAMLLDRSGSMG
                     DPNGKLMYEKKLELRFKIAQDLFEAFVKGGRGNLQGRPNDLIGLFSFATYPRTDHPFS
                     LDHESIGNMVKQLSFEKPFIDEYGQPTDDIRKAALETDERGRVLRDNWGRPVVKTNPM
                     QLTDLKAAIEYAANKLIMLDEDLARPSAGRRKYEVKSKVLVLLTDGEPTVSGGRRDAE
                     YPDEETMKKLIDAGIRAYFVQILSHQRYRERPDGTIEVIVPGGGGLFGPLVDPREAAA
                     VNKAIDEARKLARRTGGEHFLATSGDQLEAIYERIDALEKSDVGGRAVFSHEERYRPF
                     LGAALALLGVEVLLGLTWLRRLP"
     gene            complement(6246..7250)
                     /locus_tag="P5316_10995"
     CDS             complement(6246..7250)
                     /locus_tag="P5316_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85524.1"
                     /translation="MSRFVLLVLLIASMPPALTSCSKEEATNGGEAARGEGFEISKWY
                     GSGPVKFALEVSARSITTADSLVCRMTLDVAEGYEAEFPDIAFPDDVPGSILTRFDER
                     ETKEGDRRRQVREYEIEPEFEGALRFPAMEAYYHKVGEVKEERLEIEPVEVTVTATEL
                     GGDAIALRPARGLIETDRIEAMRRRIWPKALGAVAIAAALVVAIVWWVRRPKVEPPPP
                     PAHEIALKRLRELERSDLIGRHKVEPFFVGVTEIVRDYVEAAFGLRAPEQTTEEFLAS
                     LATATELARHREVLQPFLTAADEVKFARLDADETAMRRAFETAERFVRQTSRTEGGGR
                     "
     gene            complement(7298..8203)
                     /locus_tag="P5316_11000"
     CDS             complement(7298..8203)
                     /locus_tag="P5316_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRR85525.1"
                     /translation="MPTKPDTAEKTRELLKKVRRIQVRADKLVNDVIVGEYRSVFKGR
                     GMEFDEVREYIPGDDIRSIDWNVTARTGWAHVKRYVEEREMTVILLVDVSLSGRFGSS
                     ELLKIDLATEISAVLAFSAIKSNDKIGLLIFTDRVEKFIPAKKGKRHVLRVIRELLSF
                     EPQAGGTDVAGALEFLNKVLKRKAVVFLVSDFIDEDFERDLALTRTRHDLIPVRISDP
                     RETSLPDVGLIELEDAETGERVVVDTRRSRIRDLFSEAGRQEQRSLTGLLRSMGADSL
                     EISTDRPYMKDVMAFFRRRERRMKH"
     gene            complement(8313..9305)
                     /locus_tag="P5316_11005"
     CDS             complement(8313..9305)
                     /locus_tag="P5316_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR85526.1"
                     /translation="MATDVKRIGELVAAESAPIRALIDEIGKVIVGQRYMIERLVIGL
                     LSNGHVLLEGVPGLAKSLTVTTMGRAIQAKAQRIQFTPDLLPADLIGTQIYNQRTGEF
                     TVRKGPIFANIILADEINRAPAKVQSALLEAMQERQVSIGEQTFKLDEPFLVLATQNP
                     LEQEGTYPLPEAQVDRFLLKLKIDYPSRNEERQIVDRMSFTKPDGQVNAVIRPEDILR
                     LRGIVDEIYVDEKIRDYIIDIVFATRDPAAAKLPELANLIEYGASPRASIALTLAAKA
                     HAFMQGRGYVTPQDVKAIGMDVLRHRVILTYEAEAEEMGAEDVLKKVFDSIRVP"
     gene            complement(9429..10154)
                     /gene="tsaB"
                     /locus_tag="P5316_11010"
     CDS             complement(9429..10154)
                     /gene="tsaB"
                     /locus_tag="P5316_11010"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRR85527.1"
                     /translation="MSTMYTEACRILAIETSGRSGSVALATGGGQIVAAAGPLGVHAH
                     ASELMPAVERMLAGAGWPGDSLTDVFVSIGPGSFTGLRVGVTVARTLSWAVGARVVAV
                     PTLECLACNALSLIPPPMHLAVILDAKSGRVFCEAFELRNGWYAPAGDTRMDTPQAFL
                     ARCPRPLAVLGEGIAHHRQAITESGAMVLDSGLWGGKAENVVRVGVRLAEAGRYTPGG
                     DLLPCYVRRPDPEEKWEKLHREP"
     gene            10636..11427
                     /locus_tag="P5316_11015"
     CDS             10636..11427
                     /locus_tag="P5316_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR85528.1"
                     /translation="MRSQAANKTQNTNRSAKNPWYAPDPRIRSKAAVRDALNVVDFIR
                     TGTGMPEKPSDHQLFIAMHTCAYMANKSLRDAGGEHAAWTERWFMIREYIVQQNLGLA
                     YSMMSRFNTRLIDEDDLLSDAMLGLTRAVDRYNPWRGYRFSTYACNVIVRALMRRGKR
                     ERNRRRLFPVQYEVSLEQPDSLPDTERELYVERLSQVLRNNMGQLTDLETRVLAQRFT
                     GEHHDRSTFQEIGEMVGLSKERVRQIQNIAIRKLRQALAADPVLQ"
     gene            complement(11710..12090)
                     /locus_tag="P5316_11020"
     CDS             complement(11710..12090)
                     /locus_tag="P5316_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR85529.1"
                     /translation="MRILLVDDSRTIRNIQKNVLAQLGHTDISEAGDGVEALKIIAEK
                     RPDLVLIDWNMPNMDGITLVRKIRETDKSMPLMMCTTEAEKARVLEAIKAGVNNYVVK
                     PFTAETLAEKINATLAKSQQAAHA"
     gene            complement(12106..12612)
                     /locus_tag="P5316_11025"
     CDS             complement(12106..12612)
                     /locus_tag="P5316_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRR85530.1"
                     /translation="MDVRYINPFIGAVKHVFRTMLGTDIMISKPRLRAPDESNADVSA
                     VIGFSGDAAGCVILCFRTDAAVKTASKFAGNELTPQHPDFSDALGELANMVAGQAKAQ
                     FEGLSVSISLPSVIIGRDHEVLQSKITPRLVIPCDSPLGRFAVEVAMVVEKKTGATAS
                     APVAAVAG"
     gene            13502..13990
                     /locus_tag="P5316_11030"
     CDS             13502..13990
                     /locus_tag="P5316_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRR85531.1"
                     /translation="MNKKIENAFNKQINAELYSSYLYLAMSAWLESKNFKGMAHWMRA
                     QAAEEQAHALKFYDFIHDRQGRVKLAAIDEPPAEWISPAAVFKDAYEHECKVSALIHG
                     LVDLAAAEKDHAASSFLQWFVNEQVEEEAHASEIAEKLNMIGDSRGPIFMIDSELGKR
                     GN"
     gene            complement(14448..15020)
                     /locus_tag="P5316_11035"
     CDS             complement(14448..15020)
                     /locus_tag="P5316_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRR85532.1"
                     /translation="MTQQGFSRRYLDELDSVLKAIDAAAVDRAIACLREARDADRTIF
                     SCGNGGSASIASQMVVDMVKGASYRKMTRPFRMISLTDSVATITAYANDVSYESVFVE
                     PLRNWAQKGDVLIAISGSGNSPNVLRAVEFANGVGCTTIGLTTGSGGRLKDLVTLPLS
                     VPSSHMGRLEDAFFIMTHILCYAFMEDACG"
     gene            complement(15023..16075)
                     /locus_tag="P5316_11040"
     CDS             complement(15023..16075)
                     /locus_tag="P5316_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRR85533.1"
                     /translation="MNAANLSQERLAELIDRFPRQRVAVLGDFFLDKYLDIDPRIADV
                     SVESGKTAHQVVDVRTSPGAAGTVMINLASLGAGRLHAIGLTGDDGEGYDLRKALTAL
                     GCTHNHLHMGADRHTPQYLKPRDITDLSLTGEHPRFDTKNRTPTPPDLQRRIMASIDA
                     LLPEIDALVVMDQIEMDECGVVTTPVREMLAERARRFPKVVFWADSRRRIRQYRNVII
                     KSNQFEASGWPTWLPDDEVPMEELLAAAKRLREQNGVPVFVTRGARGMLVSDPWTEVA
                     GVRLDGEIDPTGAGDSASAGCVLALCAGATPPEAALIGNLVASITVQQLGTTGTVRRD
                     QLPDRLALWQKQQGYS"
     gene            complement(16072..17187)
                     /locus_tag="P5316_11045"
     CDS             complement(16072..17187)
                     /locus_tag="P5316_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85534.1"
                     /translation="MPYPRLNRFDVKMEPLSARQNKKRIETDHVPPDRMPGPLAPEAR
                     DTIAEVAERIRAARRNGRPVMVAFGAHAIKNGLAPVFIRLIEDGWVTHLATNGAGIIH
                     DWEFAYQGRSCEDVAKMVHEGRFGNWQETGFYINLAINVGAFEGRGYGESVGALIHNE
                     GLTIPSEADLIAAVNANLDSDHDLAAAAADLLGVVRRFGLPAGPMAVPHPFKRYSAQA
                     QAFRLGVPFTGHPMIGHDIIYTHPMNHGACLGRTAIRDFLTFAESVSRIEGGVYVSIG
                     SAVMSPMVFEKSMSMAQNLAIRQGRHIDGHYILVVDLAPTRWDWSKGEPPESDPAYYL
                     RYNKTFSRMGGTMRYLSADNRDFLLTLSQSLPGKPER"
     gene            complement(17230..18165)
                     /locus_tag="P5316_11050"
     CDS             complement(17230..18165)
                     /locus_tag="P5316_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR85535.1"
                     /translation="MTGPAGRGIIFAMVAYVEKDYQRVVPATGPTRFLPGTSIEIIRE
                     VRRDQPIEHVLFDFDGTLSLIREGWPEVMVPMMVEVLRATGTAESPQTLYRLAHEFVM
                     ELNGKQTIYQMIRLAEEVRRRGGTPLEPAEYKRMYHDRLMDRIRWRREALADGRMPPE
                     DMVVPGAFELLGRLRDRGLRLYLASGTDEHYVKEEVRLLRLDPYFGEHVYGAIDDYKS
                     FSKAKVIERILETNRVQGERLLGFGDGYVEIQNVKAVGGTAVAVASDEANRSGKPDPW
                     KRDRLIGVGADVVIPDFRECDPLLAYLWGDAATAG"
BASE COUNT         3406 a         6198 c         5364 g         3561 t
ORIGIN      
        1 gaaatcggct gtgtgagcgg cacctgccga ccgtcgccga gcttcaaggt cgtaatcacg
       61 ggattgcccg gctcaacgcc gcggacgacg aaactgaaag tggtcgtccc cgtcacaccg
      121 tgggcaggct ctcccacgga cttgatttca aggccgggcg gcgccaccgg cgttggctcc
      181 gactcggcgc tcagaccaag agtatccaac tgaagagttg cctcctcgcc gatcaccagc
      241 gattgcgaag aaacccgcgt cccgacaatc acaaagggcg aaacggtcag cgtgacctcg
      301 tcagcggaaa cacccgcaaa gctgattccc ctgatctgga aggtcccaag cttcgaagcc
      361 gtcacgtgat aacggaacac ccgaaccgga acgccctggt tgtcttgctg aatctgcgga
      421 tcgcccgtcg atgtgatggt taatccttca atgctcggca acacgggtcg agcaccgggg
      481 cgatgcccct gaaaggcaag aataagggtg gccggacgac cgacaagtgt ttcctttgga
      541 ttcagttcgg cggcaatgca tctgatcggg gcctccttga cccgcagcgt gaaaggcccg
      601 cgcgtgaccg tttcctcgcc gaccttgacc gccaccgcgg gaatctggaa cgtgcccgga
      661 cgagtcggat tgaccagaaa tcgatatgat cgctgcaccc tgccggagat cagatcgtgt
      721 atcaccgaag gggaaccgta tctcgtgatg tgtactcctt ccacggcctc gacgctcggc
      781 cactcgccgg tgtcatcttc aacactcacc gtgagaatcg tctgggtacc gaaatagatc
      841 gtttccgggt tcaaaccggc gttgacctgc acggccgatg cggccggtga cgcctgcaca
      901 agccacgccg cggccagagt caacagcccc atcctgttca gatgacgccg cgcaaatggc
      961 aagagaccgc aagcaagccg caacacctgc cggctgccac tggcggcttg ctgccttcgc
     1021 ggtcgaagcg gccgcttcgg tggaggaggc gccccgtcag ggttatctcg ctcccgacca
     1081 gacccgccgg ctggccgtcg ggaccactgc ctcgcttgtg tggtcctgtc cgagacggtt
     1141 gccatcacca tcaccaatcc ttgatcaccg ttcgcgggcg gcgctgatgg agctgacgtt
     1201 caactccccg gcgctgcttt tcggcctgct caagcgcccg ctgaatgtcc tgcctgtccc
     1261 tctgctgttc ctgttgctgc cgatcttctt gatcccgctg atcgtcctgc ggctgcgact
     1321 gcggcggttg caggaccatc agtgccttga tcagttcctc cagcgccttg gcctggggcg
     1381 cctggaccgg accgccggac ttcagcggct tgcgggagtt ctcaaccgaa gccttgttga
     1441 gaccctcaat cgcctgcgtc atctgttcgt cagcagcgcc cacgtgcttg cccgcctcga
     1501 tcagggcctg cacgcgcgat ggctgtgacg ccggcggagc gccggccggt tgcgaggccg
     1561 cctccgccgg gatctcatcg cgaatctgtg ccgccgcctt gccgcaatcc ttctgaatct
     1621 tctcctgacg ctcccggaat ccgtcggcga ccttcgatgc tcgcggagcc agcagcctcg
     1681 tctttaattc ggcggcccac ttgcgaacct cgtcaagcgg cgagggcttc ttggattcat
     1741 cgaccggttg gtcacccgcc tccaccttga cctgcgaatt cagctccgcc tgcttcacaa
     1801 tcagttcctg caatcgctgc tcgatggtcc gcggcagcaa ctccatcgcc tcgtcgatca
     1861 ttctgagcag ctcatcctgg gccgccagtg tgtccttgcg gctgcgctcc gtgatcgctt
     1921 tcagacatcg ctgccccgtc tccggtgcgg gctccagaat cttggacagc ttctcgttca
     1981 gctcacgatc aagcatcggg tttccctgct gctcctgggg cacctgggcc tcatcagcag
     2041 gtcgggtcga gagaattcga aactggttct gtaacaacgt caaggtaccg cctacaacgg
     2101 cttgctgaac aaagatgctg gttttgtctg cgtcccactt ggcgtccttg ggccactcga
     2161 cgtctcccag cagcggatcg ctgagccaat cggtctcttt ttttacttcg gcaaccaacc
     2221 cgcgcagcac ttgttgaatc gcccttgctt tggccaacgc cggcgccggc tcgagcggat
     2281 agaggaacaa ggccccgtac atctgttcaa gagcggcaaa ctgcttgtcc cagccgggtt
     2341 tgatctcgcc gtcaagcaga aacgcgcacg cgtccttctg cgaatcgacc gctttcctga
     2401 gctgttctga cgccgcatga taggccttct caagcggggt cttctcgacc ggctgggtgg
     2461 aagcttgcga cgtcgccgcc ccgccggcgt cggcttgctg ctggcggtac tgggccaact
     2521 gatcggccag ttcgccggtc cgctccatga gcttgcgttg gccgcgaatg gcggtgcgcc
     2581 gtgcgtgacg aaccgcggct gcatcctccg gcggtttgat caccagatat cgcgccagac
     2641 tcacctgctg atcctgctcg gctatcagcg attccagata ggcacgcgga tccttcagaa
     2701 tgtcttcctt ctccttggcc tccgtggctc gccgcaacaa gcccagtacg ttgcggcgaa
     2761 tgatccgggc tgcccgaatg ttgtgttcga cgtcgtcgtc cgggccgacg tcgcgcgctt
     2821 ggcgaaacgc cgtgatcgcc tgcagaagct cctccgcgat cttcccgaga ttctcgatgt
     2881 tcgccggatc gtcgggcgcg ggtttgcgat ccgtcggctg cgtggccgga gccatcagat
     2941 cgttcagttt ctgacgggca ctcgcagccc gcgccaggcc gacgttgtag aacgcatcct
     3001 gcttaaggta agcgggcaca cccgagtgac tcgccgtctt ctcgaactgt tgaatcgcct
     3061 tgtcgccgtc gcccaactcc atgtgacaca gccccatgtt gtaccagacg gccgggtgat
     3121 ccggctccgc ctcatcggcc tgcttgtaca gcttcagggc ctgctcaaaa cggttctgct
     3181 cgaattgctc ctccgcatcc agcagcaaag cacgaatgtc ggcccgcgcg accaccggca
     3241 gcaccgcgat cagccacaac atcaccagaa gccactggcc ccagaatcct cttcgctggc
     3301 cccagaatcc tcttcgctgg ccccagaatc ctttctgggg ccttctccct ggcagagtcc
     3361 caccctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttt ctggggcctt
     3481 ctgcctgccg gagtcccatc ttcatggtca tggaatgcgg acttcgctgg ccccagaatc
     3541 ctttctgggg ccttctccct ggcagagtcc caccctcatg gtcgtggaac ccggcggctc
     3601 gcccgccagt gcatgggcag tcaagttcgc tggccccaga atcctttctg gggccttctg
     3661 cctgccggag tcccatcttc atggtcatgg aatgcggaac ggattccgcc gcgtgagggg
     3721 gcccgaaagg gattccgggc cgagcggacg acagcgtgcc gccggcggct cgcccgccgg
     3781 cgtcctctct gcccgaatcc ggcacgggca gacacgccgc ccgtggcacc catgcgccca
     3841 tccgcgcaga cctgttcacc tggatcggtc cacgggcgat catcgcccaa cccccgcccg
     3901 cgctacgggc cgttgctcgc tgatcaacgt cgagagcacc accagcacga cgccgatgcc
     3961 tacaaacaat tgaaacttct cctgccacac cgtgatccgt acgtccttgg tctgcatgcc
     4021 ctgcttggca atgacgttct tatagatgtc cgtgagatcc acgttcgacg cccccgcggc
     4081 caggtagccg catcggtccg gcagatccgc cacaatgctc cgcagcgacg catcatcaaa
     4141 cttggtgatc acctcctgat ccttgtattt caaagtggtt atctttccgt caacttcgat
     4201 cggaatcggc gcaccggcgg ccgtgcccag gccgatgatg taaatgccga ccccgagccg
     4261 gtgagccaat gcggccattt ctctggcgtc gccctcgtga ctttcgccgt ctgtcagaat
     4321 caccagaaca ttcgcgttac gatgactgtc cggctcctca accttgagtt tcgtcgacgg
     4381 ctgggtggcg gaggccttct ttttgtcgtc aacggcctgg gtcggatcca gaccgaaaac
     4441 gttgcgaatg gctgaataga tcgcatcacc cagattggtg ccgccttcgt caacgctgtt
     4501 gaaagtgaca tcctgtaaca cccacaagag atgcgaataa ttcggcgtca gcgggcattt
     4561 caactcgctc gtgcccgcat acgccatcag ccccacctga ttgcccgcag gaagttgccc
     4621 aatgagcgaa cgaatcgcgg ccttcgccgc ttccagacgc gaaggatcga cgtcgcgggc
     4681 acgcatactg ttggagacgt ccagacaaaa gagcaggttc tggccctgct gcggcatctc
     4741 gatttgcctg ggatcccatc gtggttgcgc cgcggccaga atcaggcacg ccaaacccac
     4801 gcacaggacc gcaaaccgca cgatcggccg tccgatcgaa cggcgcgggc acacgtcatc
     4861 cagcagcgac ttggacacaa acaacgacac cgccctctgt cgacggcgca gatcgaatcc
     4921 gaacagtacc gccggcggca ccaacgccca caaccattgc agggcctccg gatggttcag
     4981 gttgaagaat tccgcaccca tctacggcaa cctcctcaac caggtcagcc cgagcaacac
     5041 ctccacgccc aaaagcgcca gcgccgcccc caaaaagggc cgataccgtt cctcatgcga
     5101 gaaaacagcc cgccccccga cgtcggactt ctctaaagcg tcgatccgtt catagatcgc
     5161 ttcaagctgg tcgccgctgg tcgccaggaa atgttcgcct ccggtccgcc tggccagctt
     5221 gcgggcttcg tcaatcgctt tgttgaccgc cgccgcctcc cgcggatcaa ccaaaggccc
     5281 gaacaaaccc ccgccgcccg gaacaataac ctcgatcgtc ccatcgggac gctcgcgata
     5341 ccgctgatga gacagaatct ggacaaaata ggcccggatg cccgcatcga tgagtttctt
     5401 catcgtttct tcgtcggggt attcggcatc ccgccgcccg ccgctcaccg taggctcacc
     5461 gtcggtcagc agcaccagaa ccttgctctt gacctcgtac ttgcgacgcc ccgcactcgg
     5521 tcgggccaga tcctcgtcga gcatgatcag cttgttcgct gcatattcga tggccgcctt
     5581 caagtcggtt aactgcatcg ggtttgtctt cacgacgggc cgcccccagt tgtcgcgcag
     5641 cacgcggcca cgctcgtcgg tctccaacgc agccttacgt atgtcatccg tcggctggcc
     5701 gtactcgtcg atgaaaggct tctcgaacga gagctgcttt accatgttgc cgatcgattc
     5761 gtgatcgagg ctgaaggggt gatcggttcg cggataggtc gcaaaactga acaggccgat
     5821 cagatcgttg ggccggccct gcaggttgcc acgcccccct ttgacgaacg cctcgaacaa
     5881 gtcctgtgcg atcttgaacc gcagctccaa cttcttctcg tacatcagct tgccgttcgg
     5941 gtcgcccatg ctgcccgagc ggtccaacag catcgcgatc gccaggccct ggctgggaag
     6001 ctcttgcgtc tccagcttgc ggaccggccg ggctaatgcc acgatcagca gcaacaaggc
     6061 cagcgcgcgc agaacaggca acaggatgcg gcaccgctga cgcaggctct ttggcaaccg
     6121 cttgaggttg cgaaccgacg gataacgcag ggcggccgtc acaggccgtc gccgcagcac
     6181 aaacaggtag agcggcacca gcagcaccag ccaaagcgcg tgcgggttgg cgaactcgat
     6241 gaacctcatc gccccccgcc ctccgtccga ctcgtctgac ggacgaatcg ctcggccgtc
     6301 tcaaacgctc gccgcatcgc cgtttcgtcc gcatccaagc gcgcaaactt cacctcgtcc
     6361 gccgcggtca ggaaaggctg cagcacttct cgatgccgag ctaactcggt cgccgtcgcc
     6421 aggctggcaa ggaattcctc cgtggtctgc tccggggccc gcaagccgaa cgccgcctcc
     6481 acataatccc gcacgatctc cgtcaccccc acgaaaaacg gctccacctt atgccgcccg
     6541 atcaggtcgc tgcgctccag ctcccgcagc cgtttcagag caatctcgtg agcgggcggc
     6601 ggcggaggct cgaccttggg acgccgcacc caccacacga tcgccacgac cagtgcggcc
     6661 gcgatggcca ccgcgcccaa agccttcggc cagatgcgtc gccgcatcgc ctcaatacga
     6721 tcggtctcga tcaatccccg ggcaggccgc aacgcaatcg cgtctccgcc tagctccgtt
     6781 gccgttaccg tcacctcaac cggctcgatc tccagccgct cctccttcac ctcgccgact
     6841 ttgtggtagt acgcctccat cgccggaaac ctgagcgcgc cctcgaattc cggttcaatc
     6901 tcatactcgc gcacctgccg ccgtcggtcg ccttccttcg tttcccgctc gtcgaaacgc
     6961 gtcagaatcg agccgggaac gtcatcagga aacgcaatgt ccgggaactc ggcctcatat
     7021 ccctcggcca cgtccaaggt catccggcaa accagtgagt cggccgtggt aatcgatctg
     7081 gccgacacct caagcgcgaa cttcaccgga ccggaaccat accatttcga tatctcgaag
     7141 ccctctccgc gcgccgcctc accgccgttc gtcgcctcct ccttgctaca ggaggtcagc
     7201 gccggcggca tcgatgcgat caacagaacc aataacacga aacgagacat ctgacccgat
     7261 tgccattccc ttcaggctga gagctcacag ctccttctca atgcttcatt ctccgctcgc
     7321 ggcggcggaa aaaggccatc acatccttca tgtacggccg atcggtcgaa atctccagcg
     7381 agtcggcacc catcgaccgc agcagcccgg tcaacgatcg ctgctcctgg cggccggcct
     7441 ccgagaacag gtcgcgaatc cggctccgcc gcgtgtcaac gaccacccgc tcgccggtct
     7501 cggcatcctc cagttcgatc agcccgacgt ccggcagcga tgtctctcgc ggatcggaga
     7561 tccgaaccgg aatcaggtcg tgccgcgtgc gagtcagggc caaatcccgc tcgaaatcct
     7621 cgtcgataaa gtccgacacc aggaacacaa ccgccttgcg tttcagcacc ttgttgagga
     7681 actccagcgc cccggccacg tcggtccccc cggcctgcgg ctcgaagctg agcaactcgc
     7741 gaatcacccg aagcacgtga cgcttgccct tcttggccgg aatgaacttc tcaacccgat
     7801 cagtgaagat caacagaccg atcttgtcgt tgctcttgat cgccgagaac gccagcaccg
     7861 cgctgatctc ggtcgccagg tcgatcttca acaactcgga gcttccgaaa cgacccgaca
     7921 agctcacgtc caccagcaag atgacggtca tctctcgctc ttcgacgtaa cgcttcacgt
     7981 gagcccagcc ggtacgcgcc gtgacgttcc agtcgatcga ccggatgtcg tcgcccggga
     8041 tgtactcgcg aacctcgtcg aactccatcc cgcgaccctt gaacaccgaa cggtactcgc
     8101 cgacgatgac atcattgacc agtttgtcgg cgcgcacctg gatccgccgc accttcttga
     8161 gcagctcgcg ggtcttctcg gcggtatccg gcttggtcgg catccctcgg gccttatcag
     8221 cttaccctgc caaacagcaa agaagcatgg gaataaacct tcggacttcc ggttccctgc
     8281 gccatcctcg tttcgtcatt cggacttcaa gcttacggta cgcggatcga gtcaaacacc
     8341 ttcttaagga cgtcctccgc ccccatctcc tccgcctcag cttcataggt gagaatcaca
     8401 cggtgacgca gcacgtccat gccgatagcc ttgacgtcct gcggcgtgac ataaccgcgg
     8461 ccctgcatga aggcgtgcgc cttggccgcc agcgtcaaag cgatcgacgc ccgcggtgac
     8521 gcaccatact caataaggtt cgccagttcg ggcaacttgg ccgccgccgg gtcacgcgtc
     8581 gcgaatacga tatcaatgat gtaatcgcgt atcttctcat ccacgtagat ctcgtccacg
     8641 attccccgca accgcagaat gtcctccggc cgtatcaccg cgttcacctg cccgtccggc
     8701 ttggtgaacg acatgcggtc cacgatctgc cgttcctcat tgcgcgaggg atagtcgatc
     8761 ttgagcttga gcagaaaccg gtccacttgg gcctccggca gcgggtacgt cccttcctgc
     8821 tccaacgggt tctgcgtggc cagaaccaga aacggctcat caagcttgaa ggtctgctcg
     8881 ccaatcgaca cctgacgctc ctgcatggcc tcaagcagag ccgattgcac cttcgccggt
     8941 gcccggttga tctcgtcggc caggatgatg ttggcgaaga tgggtccctt gcggacagtg
     9001 aactcgccgg tccgctggtt gtagatctgc gtgccgatca ggtccgccgg cagcaggtcc
     9061 ggcgtgaact ggatccgctg ggccttcgcc tggatggccc gacccatggt cgtcacggtc
     9121 aggctcttgg ccagccccgg aacgccctcc agtaacacgt gcccgttcga aagcagcccg
     9181 atgaccagcc gctcgatcat gtaacgctga ccaacgatca ccttgccgat ctcgtcgatc
     9241 agcgcgcgga tcggggcact ctcggccgcc accagttctc cgattctttt cacatccgtc
     9301 gccatagtcg caaccctcaa agactctgcc ctttccgggc tccgtaagtc gtttaatagc
     9361 aaggggtacc aatgtacttt cggctgtcga ggtttttcaa gttggtacgg gcataccccc
     9421 ggcagggttc agggttctct gtgaagcttc tcccatttct cctccgggtc aggacgccga
     9481 acgtaacagg gaagcagatc gccgccgggc gtatatcggc cggcctcagc cagacgaacg
     9541 ccaacccgca ccacattctc ggccttccca ccccacaggc ccgaatcaag caccattgcc
     9601 ccactttcgg tgatcgcctg tcggtgatga gcgatcccct cccccagaac ggccagcgga
     9661 cgcgggcagc gggccagaaa ggcctgtggg gtatccatcc gggtgtcgcc ggcaggcgcg
     9721 taccacccgt tccgcaactc gaaggcctcg caaaacaccc gtccgctctt ggcgtccaaa
     9781 ataaccgcca ggtgcatagg cgggggaata agcgacaagg cgttgcacgc caaacactcc
     9841 agcgtcggca cggccacgac ccgtgcccca accgcccaag agagcgttcg ggccacggtc
     9901 acgccgaccc gcaagcccgt aaagctccct ggaccgatcg aaacaaatac gtcggtgagc
     9961 gagtctcccg gccaacctgc gcctgcaagc atcctctcca ctgctggcat caactccgag
    10021 gcgtgggcgt ggacccccag cggaccggcc gccgccacaa tctgcccgcc acccgtcgcc
    10081 agtgccacgc tacccgatct gccggaggtt tcgatggcga gaatacgaca cgcctcggta
    10141 tacatggtgg acattctagc cccagcaggg gcaacccgcg aagaatcggt tcccgacact
    10201 tgcatctacg acccgagcgg tagattcatg gcccacggaa agcattccgc atcggccagc
    10261 tggcgccaga gcacaggact tgccgcgcac ttcggtcccg cgctgccctg ccgcgggtag
    10321 ttgctgcccc tccagccaag tgccgaaaac acagacttcg tgaccgctca gtcccaaaaa
    10381 gcgccggatc agtgaccctg atggcccgtt cccgcgggcg gggagcagat cacttgcccg
    10441 catatccaat tctataatac tttgtcaaca ataaagatac gccacattca gcacttgatc
    10501 gaagcatcgt ttgacgagtg atcttcttcg aggaaaagtt tcttagggaa atcagtattt
    10561 tcccttgcat taggaccgat ctggtgctat acttaatagc acctaatagg tcccgatagc
    10621 gaaagggatg cgaccatgag atcacaagca gcaaacaaga cccaaaacac caatcgttcg
    10681 gcaaagaatc cctggtatgc acctgatcca cggattcgca gcaaggcagc cgttcgtgat
    10741 gcactgaacg tggtcgattt catacgcacg ggcactggca tgccggaaaa accgagcgat
    10801 catcaactgt tcatcgccat gcacacgtgt gcctatatgg ccaataagtc gctgcgcgac
    10861 gccggcggcg agcatgcagc ctggaccgag cggtggttca tgatccgtga gtacatcgtt
    10921 cagcagaacc tcgggctggc ctattcgatg atgagccgtt tcaacacgcg cttgattgac
    10981 gaggatgatc tgctgagcga cgcgatgctg ggcttaacac gagcagtgga ccggtacaac
    11041 ccctggcggg gataccgctt cagcacctat gcgtgcaacg tcatcgtcag ggccctgatg
    11101 cgacggggca agcgggaacg caaccggcgc cgactgttcc cggtccaata cgaagtctcg
    11161 ctggagcaac ccgacagcct gcccgacacc gagcgggagc tgtatgtcga acgtctcagc
    11221 caggtgctgc ggaacaacat gggccaattg accgatttgg agactcgcgt tctggctcag
    11281 cgtttcacgg gcgagcatca cgatcgttcg acttttcagg aaatcggtga gatggtgggg
    11341 ctcagtaagg aacgtgtacg gcagatacaa aacatcgcca tcaggaaact ccgtcaggcg
    11401 ctggcagccg acccggtcct tcaatgacgc gccaatcagg ccgccgtcgg tttgcagacc
    11461 gttcctgttg cggtcggagc ggcctcaaag cgggggtact ttcgtgaggc tgcccttggc
    11521 tcgccaaccg aaacaggaag ttgcctctga caggcatgag cggagcccat tgcccgagcc
    11581 aagtcccaat ccgttgctgc tccgacaaag gctccgccgg tgccccgacg aacccgatat
    11641 ccttgtcagc cagacgggct tctctgcggc aacgctattg ccgtgggtat gcgttcagtt
    11701 gacaagtgtt cacgcgtgag ccgcttgctg gctcttggcc agcgtggcgt tgattttttc
    11761 cgccagggtt tctgcagtga acggcttgac gacgtagttg ttgaccccgg ccttaatggc
    11821 ctcgagcact ctggccttct ccgcttcggt ggtgcacatc atcagaggca tgctcttgtc
    11881 cgtttcgcgt atcttgcgga caagcgtgat accgtccatg ttgggcatgt tccagtcaat
    11941 cagcaccaga tcgggccgct tctcggcaat gatcttcaga gcctcgacgc cgtcaccggc
    12001 ctcgctgata tccgtgtgtc cgagctgggc gagcacattc ttctgaatgt ttcggatagt
    12061 cctggaatca tcgaccagca gtattctcat ggattgaact ccgcttcagc cagccacggc
    12121 agccaccggc gccgacgccg ttgcccccgt cttcttctca accaccatcg ccacctcgac
    12181 cgcgaaccgg ccgagcgggc tgtcacaggg aatcaccagc cggggggtga tcttcgactg
    12241 gagcacctcg tggtcgcgcc cgatgatcac gctcggcaat gagatgctga ctgagagacc
    12301 ctcgaactgg gccttggcct gtccggccac catgttcgcg agttctccca aagcatccga
    12361 gaaatcggga tgttgaggtg tcagttcatt tcccgcgaat ttcgaagccg tcttgacagc
    12421 ggcgtccgtg cggaagcaca ggatgacgca accggccgcg tcccccgaga aaccgatcac
    12481 cgccgagaca tcggcattcg actcgtcggg agccctcaga cgcggcttgc tgatcatgat
    12541 gtccgtgccc agcatcgtcc tgaacacgtg cttgaccgcc ccgatgaagg gattgatgta
    12601 tctcacgtcc atgacacgca tacctctcgt ctggcctgcc ggatactcga taaccccgtt
    12661 tcagtggcac tgcttccacg gcacgggaca cttccatatc ggtatgcggt ttgagaggct
    12721 gaatctgacg ggcgcccaga ctgcgtttta atccaccgcc ctccggcgcg atgacacgtt
    12781 atcagacgcg caccacattc gggcgccatc agagcgttcg caaactgcgg gccttgggcg
    12841 cggcgggccg ccgaaggccc gtgacgatct gcgtgggggc cactggcatc tcgtctggca
    12901 gagtcttggg ctgcccaggc cccacgtacg gacaggccgc ctgcggcacc cgaagcgcgc
    12961 tccctccggc tatgtcgcgt gcccggccgc cgacatcggt gtctcggacg cggttcttgg
    13021 ccacggacct ccgggccaaa tgatctcaaa gtgcctcgga atgaatatgt taagtttttt
    13081 tcttctttcg ggttgccggt cgcaatcaaa gatactagtg attattggcc acagtacaga
    13141 gcatcatgca tgaaccacag acgtcagacc aaacagcgag ctgcgatcct ggaagctccg
    13201 aggaagttga gaagccaacc cacagtagcc gcgcttcctt cgtttgtccc gagccagttg
    13261 ccgcggatct gcctcggaac ggtctgtgac aacctcgtgg cctcgtcgac ggatgcaagc
    13321 attcggagac ttgtcagcgg gcattttgag gcccgcttcc acgccggtgc cgccgcgctt
    13381 ggcccctcgt ccggacgagc gggcctgaga tcgatgagac aaacccagga gtcgcgagag
    13441 cctgagaacc acgcgggcat gacggcccgc ctacaacctt ttgaactaag gagccaaaga
    13501 aatgaacaag aagatcgaga atgcgtttaa caagcaaatc aacgcggagt tgtactcctc
    13561 atacctctat ttggctatgt cggcctggtt ggaatccaag aacttcaagg gcatggccca
    13621 ctggatgcgt gcccaggctg ccgaggagca ggcccacgcc ctgaagtttt atgacttcat
    13681 ccacgaccgg caaggccgcg tgaagctggc ggccatcgac gaacccccgg ccgaatggat
    13741 atcgccggcg gcggttttca aggatgccta cgagcacgag tgcaaggtct cggctctgat
    13801 tcacggcctg gtcgatctgg cggccgccga gaaggaccac gccgcctcaa gcttcctgca
    13861 atggttcgtc aacgagcagg tggaagagga ggcccacgca tcggagatcg ctgagaagct
    13921 gaacatgatc ggcgacagca ggggcccgat cttcatgatc gactccgaac tgggaaaacg
    13981 cggcaactga gcccgcggtt tctgttgtgt cagaaaacgc gcatgaaatc gtgtgtgcgg
    14041 cgattccggc ctgcgatcag caagctggtc cgcgagcgcg gaccctgcct ccctttactc
    14101 gaccagcgta tcgccgtctg ccggatcgcg cgtccgcttg gtctggacgg ccgtcaggcg
    14161 gttctccctt gacacgacgg gtggtgccgc cgcgagccag aggctccaac gcttcgcgtt
    14221 gcccggcgag tcaagggtcc ggcgtcgtag aaataccaga acctctcgtc gtcgctgctc
    14281 gattctgggg catgcacctg ggctgccgtg cagcccgtct cgcccggcca gacgcgtcgt
    14341 gtcaagggtc agtaggccgt gggactcggc ccccatggtt ggcggcaggt ctggcgacct
    14401 gccctacggg ccagtccacc gtccgcccca aggtgcctgt tcagggctca tccgcaggcg
    14461 tcctccatga aggcatagca caggatgtgg gtcatgataa agaaagcgtc ctcaagtcga
    14521 cccatgtggc tcgacggcac ggacagtggc agcgtcacga ggtccttgag gcggccgccg
    14581 ctgccggtgg tcaggccgat cgtcgtgcag ccgacgccgt tggcgaactc gaccgcccgc
    14641 aggacgttgg ggctgttgcc gctgccgctg attgcgatca gcacatcgcc cttctgagcc
    14701 cagtttcgca gcggctcgac aaagacggac tcgtagctga cgtcgttggc gtatgcggtg
    14761 atggttgcga cgctgtccgt gaggctgatc atgcggaaag ggcgtgtcat cttccggtac
    14821 gaggcgccct ttaccatgtc gaccaccatc tgcgaggcga tcgaggcgct gccgccgttg
    14881 ccgcacgaga atatcgtccg gtcggcgtcg cgggcctcgc gcagacaggc gattgcccgg
    14941 tccaccgccg cggcatcaat cgccttcagc acgctgtcga gctcgtcgag gtatcgtcgc
    15001 gaaaaacctt gttgcgtcat gattatgagt atccttgttg tttctgccac agggccagac
    15061 gatcgggcaa ctggtcccgg cggaccgtgc ccgtcgtccc tagttgctgc acggtgatcg
    15121 aggcgacgag gttgccgatg agtgcggcct cgggcggcgt cgcccccgcg cagagggcca
    15181 gcacgcatcc ggcagaggcg ctgtcaccgg ccccggtggg gtcgatttcc ccgtcgagcc
    15241 gcacgcccgc cacctccgtc cacgggtcgc tcacgagcat gccgcgggcg ccgcgcgtga
    15301 cgaagaccgg aacgccgttc tgctcccgga gccgcttggc cgccgcaaga agctcctcca
    15361 tcggcacctc gtcatcgggc aaccaagtcg gccagcccga tgcctcgaac tggtttgact
    15421 tgatgatgac gttgcggtac tggcggattc gccgccggct gtcggcccag aagaccacct
    15481 tcgggaatct ccgggcacgt tcggccagca tctcccgcac cggcgtcgtc accacgccgc
    15541 attcatccat ttcgatctgg tccatcacca cgagggcgtc gatctcgggc agcagagcgt
    15601 cgatcgacgc catgatccgc cgctgcaggt cggggggcgt cggcgtacgg tttttcgtgt
    15661 cgaaacgggg gtgctctccc gtcagactga ggtccgtgat gtcgcgcggt ttgaggtact
    15721 gcggcgtgtg tcggtcggcc cccatgtgca ggtggttgtg cgtgcagccc agggccgtca
    15781 gggccttcct caggtcgtac ccctcgccgt cgtcgccggt caggccgatg gcgtggagcc
    15841 ggcccgctcc gagcgaggcg aggttgatca tcacggtgcc ggcggcaccg gggctggtgc
    15901 ggacgtcgac cacctggtga gccgtcttgc cgctctcgac gctgacgtcg gcgattcgcg
    15961 gatcgatgtc gaggtacttg tcgaggaaaa agtcccccag cacggcgacg cgctgccggg
    16021 gaaagcggtc gatcagctcc gcgagacgtt cctggctcag gttcgctgcg ttcatcgctc
    16081 cggcttcccg ggaagagact gggacaaggt caggaggaag tcccgattgt ccgccgacag
    16141 gtaccgcatg gtcccgccca tgcggctgaa cgtcttgttg tagcggaggt agtaggcggg
    16201 gtcggactcc ggtggctcgc ccttggacca gtcccagcgg gttggggcaa ggtcgaccac
    16261 caggatgtag tgaccgtcga tgtgccgccc ttggcgaatg gcgaggttct gcgccatcga
    16321 catcgatttc tcgaacacca tcgggctcat gaccgccgag ccgatcgaga cgtacacgcc
    16381 gccctcgatc cggctgacac tctcggcgaa ggtgaggaag tctcggattg cggtccggcc
    16441 gaggcaggcg ccgtggttca tcgggtgcgt atagatgata tcgtggccga tcatcgggtg
    16501 gccggtaaaa ggtacgccga ggcgaaacgc ctgtgcctga gcgctatagc gcttgaacgg
    16561 gtgcggcacg gccatcggac cggccggcag cccgaagcgc cgaacgacgc cgagcagatc
    16621 ggccgccgcc gccgcgagat cgtggtcgct gtcgaggtta gcgttgacgg ccgctatcag
    16681 atcggcctcc gaagggattg tcagcccctc gttgtggatc agggctccaa ccgattcgcc
    16741 gtatccgcgg ccctcgaacg ccccgacgtt gatcgcgagg ttgatgtaga aacccgtctc
    16801 ctgccagttg ccgaagcggc cctcgtggac catcttcgcg acgtcctcgc aactacgccc
    16861 ctgataagcg aactcccagt catggatgat accggccccg ttggttgcca ggtgcgttac
    16921 ccagccgtcc tcaatcagcc ggatgaacac cggggctagt ccgttcttga tcgcgtgagc
    16981 gccgaaagcg accatcaccg ggcgcccgtt gcgccgcgcg gcccggatgc gctcggccac
    17041 ctccgcgatc gtgtcgcggg cctccggggc cagcggcccc ggcatgcggt cgggcggcac
    17101 atggtccgtc tcgatccgct tcttgttctg ccgcgccgag agcggctcca ttttcacgtc
    17161 gaagcgattc agccttggat aaggcatccg actatcctca cgcggcccgc gccgcgccaa
    17221 tgacttggcc tatcctgcgg tcgccgcatc gccccacagg tacgccagca gcggatcaca
    17281 ctcgcgaaag tcggggatca ccacgtccgc ccccacgccg atcagtcggt cgcgcttcca
    17341 tgggtcgggc ttgccgctgc ggttggcctc gtcgctggcg acggccaccg ccgtcccgcc
    17401 gacggccttg acgttctgga tctccacgta gccgtcgccg aagcccagca gccgctcgcc
    17461 ttgcacgcgg ttggtctcga ggatccgttc gatcactttc gccttcgaga acgacttgta
    17521 gtcgtcgatc gccccgtaca cgtgctcgcc gaagtacggg tcgagccgca gcaggcggac
    17581 ttcctctttt acatagtgct cgtccgtccc gctggcgagg tacaggcgca ggccgcgatc
    17641 gcgtagccgc ccgagtagct cgaatgcccc cggaacgacc atgtcttccg gcggcatccg
    17701 gccgtcggcg agagcctcgc ggcgccagcg gatccggtcc atgagccgat cgtggtacat
    17761 ccgcttgtac tccgccggct ccagcggcgt gccgccgcga cgacggacct cttcggcgag
    17821 ccggatcatc tggtagatgg tctgcttgcc gttgagttcc atcacgaact cgtgggcgag
    17881 cctgtagagc gtctggggcg actcggcggt gccggtggcc cggagcacct ccaccatcat
    17941 tggcaccatc acctcgggcc agccctcgcg gatgaggctc aacgttccgt cgaaatcgaa
    18001 cagcacgtgc tcgatcggct gatcgcgccg cacctcgcgg atgatctcaa tgctggtccc
    18061 gggcagaaac cgcgtcggcc ccgtggccgg caccacgcgc tggtagtctt tctccacgta
    18121 ggcgaccatc gcgaagataa tacctcggcc cgcgggccct gtcatccggc ccgcccccaa
    18181 taacgttgac gggccagctt cgacagcaca tacctgtatc attatcattt gccgagagcg
    18241 cggtttacga tttgaaggca ggcctttggc actgcaaggt tctgcgcgct ttaaggcgtg
    18301 gcaaagcggt gttgccgggc ggtcataacc agccatcctg aggagcgggc gtatggcgta
    18361 aagcggttca aaaccggcca aacccgcccg ccgggtcatc gggtcctccg agcaggtgcg
    18421 agccttattg gtacaattcg tgtgccggcg tcgccgatga cgagttgctg gccgatcgcc
    18481 gccaacgccg tggcaagctc catccgcgag ctgcgccgtg gccggggatc cgtggttgag
    18541 aacctttgga gattccgcca cggcggcacc aaggctgagg agagggctgg cgggcgggga
    18601 cttgaacggg ggagggatcg gtgctattg
//
LOCUS       DAOTPW010000105        18600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3032_length_18600_cov_5.713831, whole genome shotgun sequence.
ACCESSION   DAOTPW010000105 DAOTPW010000000
VERSION     DAOTPW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18600
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3032_length_18600_cov_5.713831"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1188)
                     /locus_tag="P5316_11055"
     CDS             complement(208..1188)
                     /locus_tag="P5316_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRR85536.1"
                     /translation="MTARHWQAYVLLAAMLAIVTGISIAVGTTGVPFSRTWNELGLPR
                     LLMGMLAGASLAVAGAMFQSLFRNPLASPYTLGVSSGASLGAAVVIMLVGGGLWHGVP
                     AVSLAAFAGAVVCMSVVYMVANLRRGGGIGTLLLAGITIGFICSALIVMVLFLARRYD
                     LEAILQWTMGSLQVVGFDPLYEILAMAALGGGVAAWLHRDLDLLMMGEVVAAGRGVSV
                     RRSRLVVYFAASLLTAGVVAQCGPIGFVGLMIPHLARYMVGPRHRYLLPACVLTGAAF
                     LPLCDLLARNAMWWLTGDSRQVPVGVLTNLIGGAFFLYLLLRGRGGVGGM"
     gene            complement(1198..1965)
                     /locus_tag="P5316_11060"
     CDS             complement(1198..1965)
                     /locus_tag="P5316_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR85537.1"
                     /translation="MTIEAEKLTIRRLGRTVLREVSLRIDEGDCLSIIGPNGAGKSTL
                     MGGLLGLIPAASGDVRLDGRPLGCFSRREIACRIAYVPQIHEGYMGFRVRDVVEMGRY
                     AHVRPLDPLSDVDHRAIAHAVEACRIDDLLDRTMDTLSGGERQKVWIAAALAQQAPVM
                     FLDEPTNALDPAHQAELIRIMRHLAGGAHTLVVICHDLNLPIALGGRVVAIREGRIMF
                     DDRVETLLDTSRLRELFGTEFVLHRGADGGVSVQLRI"
     gene            complement(2155..3387)
                     /locus_tag="P5316_11065"
     CDS             complement(2155..3387)
                     /locus_tag="P5316_11065"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRR85538.1"
                     /translation="MSDPYFQALFADRIGGAQYGKGTAIYKFEKIKRAKRAAIKDFPD
                     RPLLDFGIGENDEMAPRPIREAMKVEVDRLENRGYADNGIPEYKEAAAAFMKREFGVT
                     LDPVTEVNHCIGSKPALAMLPAVFINPGDITLMTVPGYPVAGTHTQYYGGSVFRLPLE
                     EKNGFMPDLDAIPDDIRRRAKLLVLNYPNSPTGALADETFFRKAIDFALKHQIVVVND
                     AAHIMLTYGRRPLSFLQIDGAKEVGVEVHSMSKGFHMIGWRLGFVAGHERIVRAFADV
                     KDNCDSGQFMAIQKAAIVGLNDPSIPVAVRTKYERRLRKLVATLNEIGFDAKMPGGTY
                     FLYARSPKGIQGGPTFATAEDCSQFLIRERSICTVPWDDAGAYLRFSVTYQAPTEADE
                     NALMAALKDRLAGDRFVF"
     gene            complement(3557..4711)
                     /locus_tag="P5316_11070"
     CDS             complement(3557..4711)
                     /locus_tag="P5316_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85539.1"
                     /translation="MTGRSKHTSHSAVPFILLATLIPLSGPICRPATPPDGNGDECTY
                     TTLGSDITSDTTLAAGCYDVTGTLEVSAGLTIQPGSTLRFQAGCGLEVTATGYISAIG
                     TADSPIVFTGATQTRGSWSGIRFMDSSSASNRLEYVTIRYGGAAGSGGLYLSSTDETT
                     KVAISHCTLGQSAGSGLFVVGDADLTGFASNTLTQNTQGPCVLPPNVVQYLDLDSDYT
                     GNDQPSIILSEGTVTDGQIWADLGVDYQATTIEVARDLTISPGVTIAFTSGGSMNVQS
                     TGSLGAVGVPGREIILTGVTQTPGSWEGLSYTDTSSPSNNLTLVTIEYGGATRANLSL
                     NTAPVRVSANECTFAHSATCGIYVAAGATINTDVATVNTFTDNAGGDVCP"
     gene            complement(5434..7146)
                     /locus_tag="P5316_11075"
     CDS             complement(5434..7146)
                     /locus_tag="P5316_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85540.1"
                     /translation="MRCKSAIGFSRVLALVGLSLLVPLSGQMCSPGPGPGATVCIELS
                     DDIDTATTLSAACYRVTSNIDVNAPLTINPGVTLEFDAGRSMSVNSGGSLRAVGTTEN
                     PIVFTGREAVPGYWGGLTIYYSNSSNNRLEHVTIEYGGSSSGANLTTTGTATEPPRLA
                     IVNCTLRNSAGYGLYLDGDSVITDFSTNTLTGNALGAASVAQNVIGVLDAASTYSGNT
                     RDVVNVHGGSVSSAQTWQAIDADYLFDSSDLDVDADLIISAGATLVFGQARAAWINEN
                     GSLKAIGTSTAPIAFTAKEATAGYWKGVEYYYSNSVNNRLEHVTIEYGGSPSGANLTT
                     TGTASVPPRLAIVNCTLRNSAGYGLYLDGDSVITDFSTNTLTGNALGAASVAQNVIGV
                     LDAASTYSGNTRDVVNVRGGSVSSAQTWQAIDADYLFDSDDLIVDADLTISAGATLVF
                     GQGRYASVNENGSLKAIGTSTAPIIFTGKQATAGYWECLLYYYTTSVNNVLEYVRIEY
                     GGGSNSGNLRLSGNARATARNCTFAHSGGYGVYVDRNATINNDVETANQFSDNALGNV
                     FISP"
     gene            complement(7367..8215)
                     /locus_tag="P5316_11080"
     CDS             complement(7367..8215)
                     /locus_tag="P5316_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR85541.1"
                     /translation="MPLHASHPRNWRENLYVYPVISRRSGGLSIGINLNPDKACNFNC
                     VYCQVDRDAPAAVKRVDLARLRTELETTLQSAASGDLFVRPPFDRVPESQRRIRDIAF
                     SGDGEPTGAPEFPSAVQIVAELRRACGLSDAKTVLITNAAFLHRPPVREALALMDANN
                     GEIWAKLDAGTEEYFRGVNRPSVPLQRVLDNILEVSRVRPVVIQSLWMKLHDRPPPQA
                     EVEAFAQRLAWLVRVGGRIRLVQVYTVARQTAEPFVAPLDDDALRDIADRIKAIVDVP
                     AEIYGS"
     gene            complement(8357..10273)
                     /locus_tag="P5316_11085"
     CDS             complement(8357..10273)
                     /locus_tag="P5316_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR85542.1"
                     /translation="MSLPGCSKPVCGLIAFLLLIQVIGCESSLRDAGRPSADPHGRRA
                     RMVAEARLSADEPAATRPSGDVPSRMIAPAAIPPLSQEEVIDRQIPDPKDLPEALRKQ
                     LARTIEAINRNDRLTAEQKTQQIDEKRANFEKRLPGIENRLKVIERPHEIRLSFADAM
                     RRTLVNSYGIAAQSYNPAIHTARVVEAEAMFDEVFFANFQNNKQDRPSSSRLESNNSQ
                     LRTFEGGVRKMLPTGMLVQFSYAWNRSETDLVFQTFNPTYFNSFVVQFQQPLLRGFGL
                     DYNRAQIELTKLDREMSIERLKRDVREILFSVEQAYWQLLQARRVIGVVSQLLSDLEI
                     IHKDLKERFEAGYDVYGVQLSFIQSRIERHEAEFVALTKRVRDAEDALKRLINDPEMD
                     LSKDVGIIPVDLPLIEPVVLDQIGEIAVGLSNREELREAKLAIEKAEIGIGVAKNQAL
                     PKLDLLFRYVVDGLGTNSDRAFSQLSENDFHEYVVGLQFEWPIGNRGPEAKLRQARLQ
                     QAQAIAAYRDQIENVILEIQQAIRMIQSEYEQVGPSYRWVTSSEEWTEATKARQERRD
                     APNLQIEMDSYESLSMGRRQLLDSLVRYNVALSNLERQKGTLLEYNNIVIRGAEDPKQ
                     MAPYRPVQGGGLAE"
     gene            10688..12370
                     /locus_tag="P5316_11090"
     CDS             10688..12370
                     /locus_tag="P5316_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85543.1"
                     /translation="MTTERDESQDTVQQPQATPLRVKQRPAQSIQEYHRYCPGEERIR
                     ISDAICFGRRRSNFPKCVGCQFNDDEKKDKPVPVPLPGAAASIEEADRRRIERLFRQC
                     DILGTCPDMLDQEMAWRVGLATSLFLKSELRGYDRSQVEKATVVVGIDMRKTSRELAE
                     SLIEGLRSGGSPVIDIGMIDTPQLYFAVNRLGACGGVQVTGGNRPATHNGFKLCGQKG
                     RTITSETGLVKISKTVLNTTRHSAPQMPDLRRLDLSGPYKEYVRSFLADPVTGFDSER
                     PLKLVVDASNGMAGRWLPIIFDDVEWLEITPLNFEHNGEFSHDPDPLVEDNLKPLQER
                     LLRINAELGVCFDGDADCCVFVDNKAQPIRADTLGALLARELLKSNPGQSVIYDLRSS
                     RALPEEIRKAGGLPRRERGNQAFVKKAIADAKGLFACELNGRYYYRDNFYCESAIITL
                     VHVLNILTETGRPLDELVASLKRYARSGERRFRLDGKTDIVAVLSDKYNNGSINYLDG
                     ITVIFEDWWFNARPAADRSFVELNVEAINEAMLKAKLAELSQLLGDPLVNGA"
     gene            12448..13710
                     /locus_tag="P5316_11095"
     CDS             12448..13710
                     /locus_tag="P5316_11095"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRR85544.1"
                     /translation="MTPGDEHVHYLRLANGIDLAVLPTGRRSVTAIEMRFLGGYAYER
                     PDRLGVAHILAETMTKGTQRRDGRALNDAFDEIGTSYAVSAARETFVISCVCLPEFVA
                     QAVELLAEMINEPSFPGEACEVAVELARQSLAALEDDPLELAKKCLCQQAYSPPLNRH
                     VYGEPATVERIGREDVVEHWRTYLKPDRLLVSVAGAVDPSAVADLFERLFPATGLNDP
                     VVNASLPLSFPLGFRPGQSHRQKDLEQEQIGVCFPGASAVDEDEAVERVIIGLLSGGM
                     SSRLWQSIREKQGLVYWVGAWFDRPRTGGMIHLGSSCTPQNLEATSEMLLKEVNRLAD
                     DVTESEVQRAISGILASAQTHGDVTRAKATRIVNDLFYLGRPIPLEEKLSRIRAVTIG
                     DVRQYLRRHPREQLSIVTVGPRCLKDFR"
     gene            13745..14992
                     /locus_tag="P5316_11100"
     CDS             13745..14992
                     /locus_tag="P5316_11100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRR85545.1"
                     /translation="MAHTRFFQETLANGLIVAVEVMPETRSAACGFLVRAGSRDDPPR
                     LAGMSHFLEHMCFKGNERLTSEDLNIGFDEIGADTNAQTSKDQTFYYSWARCEDLGRQ
                     IELLAELMRPSIPAGEFEIEKDVILEEIATYKDDLSALAYDSLYEELCPGHPISRPVL
                     GYEETIRVMTPEDMRDYLGRKYAASNMILIITGNVDPAEVMGLARRYCGSWPSVINKG
                     RDGHPPALRTGATGRIIDRFHQQAVAVAFPSVPTTHALDETAEAVAAILGGSNSRFFW
                     NIHQKGLATRVSVIREEYADFGVIILVGLCEPENAERLLEAMRREAEQLTRDGPEPKE
                     IRRVKNHRRTMLTSESEAPGYRLGQIADDIDYRGSPRTMEERLAEVDAVSADTIATYL
                     RDYPIVGEGFVATIGPRMWLSAK"
     gene            15023..15943
                     /locus_tag="P5316_11105"
     CDS             15023..15943
                     /locus_tag="P5316_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRR85546.1"
                     /translation="MEPTTPDEHDIAPPPQGGPPDSPPDAEGHAGLAVTRPPIAWMAP
                     GMPFARPITITVLWPDAPVCVPGRAPAETEQPLSRARAVIEIILAVPVAAVGAVVGYS
                     VGKSFEPADERWLRIADSMGLGLGAVLACILLVWLGNRKISSIGLTMRQWHLDLGIGF
                     ASLLATWVVLLAFGGVIVVLFPSVLQEQSAAQKAIEASFPKLSWQQMLALCLCIAIYE
                     EIVFRGFLLTRLHALVRRWWLAVVIGAVLFGLPHHYQGWWAVCGITLLGLIMGSLFAW
                     RKSLVAPITMHLFHNFAVFMLLDLVSGMRN"
     gene            complement(15995..>18600)
                     /locus_tag="P5316_11110"
     CDS             complement(15995..>18600)
                     /locus_tag="P5316_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR85547.1"
                     /translation="IENLTCVVKSLDTGPYGQRRFAIVISNAGSQDIRGVLLPPFTRW
                     TDAPEQVQQACLDHFALCPPEFVAGGVGRHPVWVPVEFSRDVFATMPATTGRAYVCSL
                     LTRDHGEGQGTEVILKPGRSATYTLHLDVGIGNRSEALSETYRRRGGYRVDPSKYDYS
                     EYNHPDLAWSRNIVVGWLNWAWDQEVMDPRTGRYRLLESLGNARRLFGGYDVFMIWPF
                     WPRAGWDARYQFEHFRDMPGGLEGLAEQVRKAETMGTRTIIAHCIWSETDRDPSQQGH
                     LDSYRELVNTAHAVGAHGVLMDIMDTTPPEIREMARKSGRELLPYAEGDNGWYQSQTN
                     LLGRIHNVHKMPYFNFKKYLLPHQPQLRVCQDGAAGRLLRRDFVLSFFNGHGVEINTM
                     FPEKRPSCQEDFGVLARAVNILRTNRANFSSLNWEPFVGSLDREVWVNRWPAAGKTIY
                     TLCGTDPGGHQGVLLRLPHRPEVHYVDLWNDRPVDVRREGDHDLLSVRIEPYEAGRQG
                     GDGDFSAGCIAVLPRVLDAELEFEMLKVTVDRMSLPAVVEIWCNRVRPDTNPLRVPAA
                     ERVQLDLYQAFGRHTNDAIVVRLLDANNQLLDQVVLPESVLRYFRIDKPGRTAPVDPE
                     SPPAGMIRVPGGEFSYVVKHGATTWIPCYHTLDQYGPGEASKPRTVHIEPFWIDRSPV
                     TNAEYAEFVRVTGYNPKDATHFLHHFVDGKPPVGHEDHPVVYVSYDDCVAYASWAGKR
                     LPTEEEWQFAAGGEDGRPWPWGFDEPQAARCPIDAKGTSAVLKHPRGASPFGVEDLVG
                     NVWQYTASLSDNGRHQVVFLRGGGWYQPPGGHWWVRGGPRRIYDHHPLPLFGPGMNRL
                     ATVGFRCVKDE"
BASE COUNT         3712 a         5763 c         5649 g         3476 t
ORIGIN      
        1 cggattcatt tggaggcgca tgcccacgca aagccgtggg catggcaccc ggcgctctgc
       61 gaaccagaaa catgcgcgca aaatgcgcac cagttgcgac ggaagagact ataaggcgga
      121 ccgccttttt gcgggccgca acccaagcgt accgcgatcc agaaacatgc gcaagccgca
      181 cacatatccg gccgtaaggt tccaagttta cattcctcct accccgcccc ggccgcgaag
      241 cagcaggtac aggaagaacg cgccgccgat caggttggtc aggacgccga ccggtacttg
      301 ccgggaatcg ccggtcagcc accacatcgc atttcgggcc aggaggtcgc ataacgggag
      361 aaacgcggcc ccggtcagga cgcaggcagg cagcaggtac cggtgccgcg gcccgaccat
      421 atagcgggca aggtgcggga tcatcaatcc gacgaagccg atcggaccac actgagcgac
      481 cacgccggcc gtcaacaagg aggcggcgaa gtaaaccacc aggcgtgaac ggcgcaccga
      541 cacgccccgc ccggcggcaa cgacctcgcc catcatcaac aagtcgagat cgcggtgcag
      601 ccacgcggcc acccccccac cgagggccgc catggcgagt atttcataca gaggatcaaa
      661 accgacgacc tgcagcgatc ccattgtcca ctgcagaatc gcttccaggt cgtagcgccg
      721 ggccaggaaa agaaccatca cgatcagggc cgagcaaatg aacccgatgg tgataccggc
      781 cagcaacaga gtgccaatgc cgccgccccg acgaaggttc gcgaccatgt aaaccacgct
      841 catgcagacg accgctccgg caaaagccgc gaggctgacc gccggtacgc cgtgccataa
      901 gcccccacca acgagcatga tgacgacggc cgcgcccaac gatgccccgc tggagacgcc
      961 gagcgtataa ggcgaagcca acggattgcg gaacaacgac tggaacatcg ctccggcaac
     1021 ggccagcgac gcgccggcca gcatgcccat cagcaaccgc ggcaaaccga gttcgttcca
     1081 agtccgcgaa aacggtacgc ccgtggtccc taccgcgatc gatatgcccg ttacgatcgc
     1141 cagcatcgcc gccaggagga cgtaagcttg ccaatgcctt gcggtcatgt aagagcctca
     1201 gatacgtagc tggaccgaga ccccgccgtc tgcgccacgg tgtaacacga actccgtccc
     1261 gaacaactct cgcaagcgcg aggtatccag aagggtctcg acgcgatcgt cgaacatgat
     1321 ccgcccctcc cgaatcgcca ccacccggcc acccagggca atgggcaggt tgagatcatg
     1381 gcagatcacc accaaagtat gagccccccc tgccagatga cgcataatcc ggatcagttc
     1441 cgcctgatgg gcaggatcca aagcgttggt cggttcatca aggaacatca ccggagcttg
     1501 ctgggccaag gctgccgcaa tccaaacctt ctgccgttca ccgccggaaa gggtgtccat
     1561 tgtgcggtcg agcaggtcgt cgatacgaca ggcctcaacg gcatgcgcga tcgcccgatg
     1621 gtcaacgtcg gaaagcgggt ccaagggacg cacgtgggcg tagcgaccca tctcgaccac
     1681 gtcgcggacc cggaaaccca tgtaaccctc gtgtatctgg ggaacgtagg cgatacgaca
     1741 agctatttcg cgccggctga agcaaccgag aggtcggccg tcgagacgca catcgccgct
     1801 tgccgccggg atcagtccca gcagcccgcc catcagcgtg gatttgcccg cgccgttggg
     1861 accaatgatc gacaggcagt cgccttcgtc gatccgcagg gacacctccc gcaggacggt
     1921 ccggcccagt cgacggatgg tgagcttctc ggcctcgatg gtcataggcg ggaattgtag
     1981 ccgagcgggg caaggaagca gaagggttca gggctcaggg ctcagggctc agggctcagg
     2041 gttcagggtt cagtgaagtg gtccatgccc agagctttgc gtgggcgtgc gggcttgatt
     2101 tcagatccgc aatctcaggt ttcagattag cccggcggtc tgaaccctga accctcagaa
     2161 gacgaaccga tccccggcca gccggtcctt cagggcggcc atgagggcat tctcatcggc
     2221 ctcggtcggc gcctggtaag tgacggagaa tcgcaggtag gccccggcgt catcccacgg
     2281 cacggtgcag atcgaccgct cgcgaatcag gaattgcgag caatcctcgg cagtcgcgaa
     2341 cgtcggtccg ccttggatgc ccttgggcga acgggcatag aggaaatacg tccctccggg
     2401 catctttgcg tcaaaaccga tctcattgag cgtggccacc agcttgcgaa gtcgtcgttc
     2461 gtacttggtg cgaacggcaa cgggaatcga tgggtcgttc aggccgacaa tcgccgcttt
     2521 ctggatggcc atgaattgcc cactgtcgca attgtctttt acgtccgcaa aagcccgcac
     2581 gatccgctcg tggccagcca cgaagccgag ccgccacccg atcatgtgaa agcccttgct
     2641 catggagtga acctcgacac ccacctcctt cgccccatcg atctggagga agctcagagg
     2701 cctgcgtcca taggtcagca tgatgtgggc ggcatcgttg accacgacga tttggtgctt
     2761 aagggcaaaa tcgatcgcct tacgaaagaa cgtttcatct gccaaggccc cggtcgggct
     2821 gttcgggtag ttgagcacca gcagcttggc gcggcggcgg atatcatcgg gtatcgcgtc
     2881 gagatcgggc atgaagccgt ttttttcctc gagcggcagc ctgaagacgg agccgccata
     2941 gtactgggtg tgcgtgccgg ccacggggta gcccgggacg gtcatgaggg tgatgtcgcc
     3001 cggattgata aaaacagccg gcagcatggc cagagcgggc ttagagccga tgcaatgatt
     3061 aacctcggta accggatcga gggtgacgcc gaattctcgc ttcatgaaag cggcggcagc
     3121 ttccttgtac tcggggatac cgttatcggc gtaaccacgg ttctccagcc gatcgacttc
     3181 gaccttcatg gcctcgcgga tcggcctggg ggccatttcg tcattctcgc caatgccgaa
     3241 gtcgagcagc gggcggtccg ggaagtcctt gatcgcggcc cgcttggccc gcttgatttt
     3301 ctcgaacttg tagatggccg tgcccttgcc atactgggcc ccgccgatcc ggtccgcgaa
     3361 caacgcctgg aaatagggat cgctcatcgc tcgtccgtcc tcgaaacgaa agacaaaggc
     3421 cggccttcag aagaccggcc ttcagggtcc atatcaataa cacgtaagcg attccgatac
     3481 aacccgccgc tgcggatcgc aaaggcggac cggcgggcgg gtgaattacc ttgatgactg
     3541 ggccgccacc gcaggcttac gggcacacgt cgccgccggc gttgtcggtg aaagtattga
     3601 ccgttgcaac gtcggtattg atcgtggcac cggcggcaac gtagatccca caggtggcgg
     3661 agtgcgcgaa ggtacactca tttgcagaaa cccggaccgg tgcggtattc aggctgagat
     3721 tggcgcgcgt cgccccaccg tattcgatgg tcaccagcgt caggttgttg cttggcgagg
     3781 aggtatcggt gtagctcaag ccttcccagg agccgggcgt ctgcgtcacc cccgtcaaga
     3841 tgatttcacg tccggggacg cccacggccc ccaacgaacc tgtgctctgc acgttcatgg
     3901 aaccgccact ggtgaaggcg atggtcacgc ccggcgaaat ggtgagatct ctggcaacct
     3961 caatggtcgt ggcctggtag tcaactccca ggtcggccca gatctggccg tcggtgaccg
     4021 ttccttcgct cagaatgatc gagggctggt cattgcccgt gtagtcgctg tcgagatcaa
     4081 ggtattgcac cacgttgggc ggcaacacgc atggtccctg cgtattctgc gtcagcgtat
     4141 tggaggcaaa gccggtgaga tcagcatcgc ccaccacaaa caaaccgcta ccggcagatt
     4201 gccctagcgt gcaatgggaa atggcgacct tcgtcgtttc gtcggtggag gacaaataca
     4261 aaccgccgga accggccgca ccgccgtatc gaatggtcac gtattcgagt cgattgcttg
     4321 cggagctgct gtccatgaac cgtattccgc tccatgaccc gcgggtttgg gttgctccgg
     4381 tgaagacgat gggcgaatcg gccgtgccga tcgcgctaat gtatccggta gcagttactt
     4441 ccagcccaca cccggcctgg aaacgcagcg tactgcccgg ctggatagtc agcccggccg
     4501 agacctcaag agtgccggtg acgtcgtaac agccggccgc cagggtggta tccgacgtga
     4561 tgtcggaacc cagcgtcgtg taggtgcact catcgccgtt gccatccggc ggagtagccg
     4621 gcctgcaaat cggtccactc agaggaatca gggtcgccag taaaatgaac ggaaccgcgc
     4681 tgtgggacgt gtgtttgctt ctgcctgtca tgatctggtc actccttcaa aagcaccttg
     4741 tctcaaactt gccgggcctg tgacggcgcc gcgtcaccat gccgacagta tacggtacgg
     4801 ccgacgccct gtcacgctgg catcccactg agcacgcctc ctggggacta ctgccagcct
     4861 cttctcgacc gctcggccta ctgggaaccc aaatcgcccc tgtcactgca tcgactagcg
     4921 gggagcctgt cgccagcttc tcaggcgggt cgaagccggc cgaacaggca tctgagcctc
     4981 agagacctga ggcggaccgc cttcttgcgg gccgcaggcc aagcgcgcgt agaccggcaa
     5041 tatgcgcgcg aaccccaccg atgtccggcc gcaaggattc aaaggcgcat gcccacgcaa
     5101 agccgtgggc atggcacccg acgatcggga gacatgcgcg caaaatgcgc accagtcgcg
     5161 acggaagaga ctaaggcgga ccgccttctt gcgggccgca ggccaagcgc gcgtagaccg
     5221 gcaatatgcg cgcgaacccc accgatgtcc ggccgcaagg attcaaaggc gcatgcccac
     5281 gcaaagccgt gggcatggca cccgacgatc gggagacatg cgcgcaagat gcgcaccagt
     5341 tgcgacggaa gggactaaaa ggatccgcct ccggcaggcc gttccggccg ccggaggcga
     5401 attcaagcga gactcggaaa ccacggagac cgctcacggc gagataaaca cgttccccaa
     5461 ggcgttatct gagaactggt ttgccgtctc gacatcattg ttgatggttg cattcctgtc
     5521 cacataaacg ccgtatccgc cgctatgagc aaacgtgcag tttctggccg tggcccgtgc
     5581 gtttccgctg agacgcaagt ttcccgagtt gctgccgccg ccgtactcga tcctcacata
     5641 ttccagaacg ttgttgaccg atgtggtgta gtaatagagc aagcattccc agtagccggc
     5701 ggtcgcctgt ttgccggtaa agatgatcgg ggcggtcgag gtaccgatcg ccttcaatga
     5761 gccgttctcg ttaaccgacg cataccggcc ttgcccgaaa accagcgtcg caccggcaga
     5821 aatggtcaag tccgcgtcaa caatgagatc gtcggaatcg aacaggtaat cggcgtcgat
     5881 cgcctgccag gtttgcgcac tgctgaccga gccgccgcga acattaacca cgtcccgggt
     5941 gttgcctgaa taggtactgg ccgcgtccaa cactccgatc acgttttggg ccaccgaggc
     6001 ggcacccaat gcatttccgg ttaacgtgtt ggtcgagaag tcggtgatta cgctgtcccc
     6061 gtcgaggtac aggccgtacc cggcgctgtt gcgaagcgtg cagttgacga tcgccagacg
     6121 cggcggcacg cttgccgtgc cggtggtggt caggtttgct ccgctgggac tcccgccgta
     6181 ctcgatggtc acatgctcga gacggttgtt taccgagttg gaatagtaat actcgacgcc
     6241 tttccaatag ccggccgtcg cctctttggc ggtaaaggca atcggggcgg tggaggtacc
     6301 gatcgccttc aatgagccgt tctcgttaat ccacgccgct cgggcctgcc cgaaaaccag
     6361 ggtcgcgccc gccgaaatga tcaggtccgc gtcaacgtcg aggtcgctgg agtcaaagag
     6421 gtaatcggcg tcgatcgcct gccaggtttg cgcactgctg accgagccgc cgtgaacatt
     6481 aaccacgtcc cgggtgttgc ctgaataggt actggccgcg tccaacactc cgatcacgtt
     6541 ttgggccacc gaggcggcac ccaatgcatt tccggttaac gtgttggtcg agaagtcggt
     6601 gatcacgctg tccccgtcga ggtacaggcc gtacccggcg ctgtttcgaa gcgtgcagtt
     6661 gacgatcgcc agacgcggcg gctcggttgc cgtgccggtg gtcgtcaggt ttgctccgct
     6721 ggaactcccg ccgtactcga tggtcacatg ctcgagacgg ttgttgctcg agttggagta
     6781 gtagatcgtc agcccgcccc aatagcccgg aaccgcttcc cggccggtga acacgatcgg
     6841 attctccgtt gtcccgacgg cacggagcga ccctcccgaa ttgacgctca tcgaccggcc
     6901 cgcgtcgaac tccaaggtca cgcccgggtt gatggtcagg ggcgcgttca cgtcgatatt
     6961 ggaagtcacg cggtaacatg ccgccgacaa ggtcgtggcc gtgtcgatgt cgtcggaaag
     7021 ctcgatgcac accgtggccc ccggcccagg accgggacta cacatctggc cgctgagcgg
     7081 caccagtaag ctcaggccga ccaatgccag cactcgcgag aacccgattg cagatttgca
     7141 tctcatcacc agtttcctcc tgttaagcag gccaacaatc aagaacacta ccctacccta
     7201 tggagaagcg cgctcgcaag aggcgccaat tgccgaacgt cggattccaa cccatcatgc
     7261 ccatcatgaa cacgacgacc tgatcatcgg atgcaacatg acgcatatta gagcagggcc
     7321 ggccatctgt ccagcggcgg gaacggcttg tcgtgcgagc gtcgtatcac gagccgtaga
     7381 tctcggcagg cacatcgaca attgccttga tccggtctgc aatatcgcgt agtgcgtcat
     7441 cgtccaaggg cgcgacgaac ggttcggccg tttggcgagc gacggtatac acctgcacaa
     7501 ggcgaatgcg gccgccgacc cggacaagcc aggcgagtct ctgggcgaag gcttccacct
     7561 cggcttgcgg cgggggccgg tcgtgcagct tcatccacaa ggactggatc accaccggcc
     7621 gcacgcgtga gacttcgagg atgttgtcga gcactcgctg cagagggaca ctggggcggt
     7681 tgaccccgcg aaagtattcc tccgttcctg cgtcaagctt ggcccagatc tcgccattgt
     7741 tggcgtccat gagggccagg gcttcccgca cgggtggtcg atgcaagaag gcggcgttgg
     7801 tgatcagcac ggtcttggcg tcgctgaggc cacaggctcg acgcagctcg gcaacgattt
     7861 ggaccgcgga gggaaactca ggtgcgccgg tgggctcgcc gtcgcccgag aacgcgatgt
     7921 cacggattct tcgctgcgat tccggcacgc ggtcaaaggg ggggcgaaca aaaagatccc
     7981 cgctcgcagc ggattgcagc gtggtttcga gctcggtccg aagccgggcg aggtcaactc
     8041 gcttgaccgc cgccggtgcg tcgcggtcca cctggcagta gacacagttg aaattacagg
     8101 ccttgtccgg attcagattg atgccgatgg acaagccgcc gctgcgacgg ctgatcaccg
     8161 ggtaaacgta aaggttttcc cgccagtttc gcgggtggct cgcgtgaagg ggcacggggg
     8221 tgtgagatcc ttccgttgtc atagaatcag ccgtctcgcg ttgacaaggg ccgctacatt
     8281 gtagtcggga aatgctcgcc ggcctgcagc cggccaaaaa cgttcggccg gcggcggcaa
     8341 caagtatcgc aggcgcttac tccgccaggc cgccgccctg caccggccga taaggggcca
     8401 tctgcttggg atcctcggca ccgcggatca cgatattgtt gtactcgagc agcgttccct
     8461 tctgccgttc gaggttgctg agggcaacgt tgtatcgaac cagcgagtcg agcagttgtc
     8521 gacggcccat ggacagcgac tcgtacgagt ccatctcgat ctgcagattg ggtgcgtctc
     8581 gtctttcctg gcgggccttg gtggcttcgg tccattcctc ggacgatgtc acccaccgat
     8641 agctggggcc gacctgctca tactccgact gaatcatccg tatcgcctgt tggatttcga
     8701 gaatcacgtt ctcgatctgg tcgcggtagg cggcaatggc ctgggcctgc tgaaggcggg
     8761 cctgccgaag cttggcctcg ggaccacggt tgccgatcgg ccactcgaac tgcaaaccga
     8821 cgacatactc gtgaaaatcg ttttccgaca actggctgaa cgcccggtcg gaattggtgc
     8881 caaggccgtc gaccacgtac cggaacagga ggtcaagttt cggcagcgcc tggtttttgg
     8941 ccacaccgat tccgatttcg gccttctcga tcgcgagttt ggcctctcgc aactcttcgc
     9001 gattggagag ccccaccgca atctcgccta tctgatccag gaccaccggc tcgatgagcg
     9061 gcagatcgac agggataatg ccgacatctt ttgagagatc catttccgga tcgttgatca
     9121 accgtttcag cgcatcctcc gcatccctga ctcgcttggt cagggccaca aattccgcct
     9181 cgtgccgctc gatccggctc tggatgaagc tgagctgcac cccgtagacg tcgtaaccgg
     9241 cctcgaaccg ttctttgagg tccttgtgga tgatctccag gtcgctcagc aactggctga
     9301 cgacgccgat cacccgccgg gcctgcaaca actgccaata ggcctgctcg acgctgaaca
     9361 ggatctctcg cacatcgcgc ttgagtctct cgatgctcat ctcgcggtcg agcttcgtca
     9421 gctcgatctg ggcacggttg taatcaagcc cgaaaccgcg aagcaggggt tgctgaaact
     9481 gaacgacaaa actgttgaaa taggtcgggt tgaaggtctg gaaaaccagg tccgtttcgc
     9541 tgcggttcca ggcgtagctg aactgcacca gcatgccggt gggaagcatc ttgcggactc
     9601 cgccctcgaa agttcgtagc tggctgttgt tgctttcgag ccgggagctg ctcggccggt
     9661 cctgcttgtt gttctggaaa ttggcgaaga agacctcatc gaacatcgcc tctgcttcga
     9721 ccacgcgggc cgtgtggatg gccgggttgt agctctgggc ggcgatacca tagctgttga
     9781 cgagggtgcg acgcattgcg tcggcgaagc tcaacctgat ttcgtgaggg cgctcgatga
     9841 ccttcagtct gttctcgatg ccgggcaatc gcttctcgaa gttggctctt ttttcgtcga
     9901 tctgctgagt tttctgttca gcggtcaggc gatcgttgcg attgatcgcc tcgatcgtgc
     9961 gggccaactg ttttctcaag gcctcgggca aatctttcgg atcggggatc tgccggtcga
    10021 tgacctcctc ctgggacagc ggcggaatcg ccgcaggggc gatcatgcgg gagggcacgt
    10081 cgcccgaagg ccgggtcgcc gccggctcgt cggccgacag cctggcctcg gcaaccatcc
    10141 gagctcggcg tccgtgcgga tcggcggacg gacggcccgc gtctcgaagc gaggactcgc
    10201 aaccgatgac ctgtatcagg aggagaaacg caatcaggcc acagacgggc ttggagcatc
    10261 caggcaggga catcggcact ccttgaacag ccgccaccgg ctgtcgggcc gggcagctca
    10321 ccccgcccaa tcccagctcg cgcccgaaca tgccctttcc agaggcagct tgtgcggcat
    10381 tctaatgctg ctgcagggag tgtcaatgac gcgaaacccg accgccgctc agagtcggcg
    10441 gcgaatccgg caaggatgcg ccacggtttt ttcagaaggt cctttcagaa ttgcagtcgc
    10501 gggccgctat ccccctgcaa ccccgccgcc ttgggggtct gggccctcgc ttacgctcgg
    10561 gatcccgaaa aaagctgcga aaaagcgtca tcgacccacg cgctcacgag gcaccgactc
    10621 ccgtgcaatg cctcgtcagc cgatgtacaa ttgtggaccc gcgggcccca tggggtagac
    10681 tatggccatg acgaccgaaa gagacgaaag ccaggacacc gttcagcaac cgcaagcgac
    10741 gccgcttcgg gtcaaacaaa ggcccgcgca gagcatccag gagtatcatc gctactgccc
    10801 tggcgaggag agaatccgga tttccgacgc catctgtttc gggcgaaggc gcagcaactt
    10861 ccccaaatgc gtcggctgcc agttcaacga tgacgaaaag aaggacaaac cggttcccgt
    10921 gcctttgccg ggggccgcgg ccagcattga agaggctgac cgccgtcgaa tcgaacgtct
    10981 gtttcgtcag tgcgacattc tgggcacctg cccggacatg ctcgaccagg agatggcctg
    11041 gcgcgtgggt ctggcaacct cgttgtttct gaaatcggag cttcgcggct acgaccgcag
    11101 tcaggtcgag aaggcgacgg tggttgtcgg catcgacatg cgaaagacca gccgcgagct
    11161 ggccgagtcg ctcattgaag gcctgcgcag cggaggctcg ccggtgatcg acattgggat
    11221 gatcgacacg ccccagctct actttgcggt gaatcgcctc ggcgcctgcg ggggggtcca
    11281 ggtgaccggc gggaaccgac ccgcaaccca taatggcttc aagctgtgcg gtcagaaggg
    11341 ccgcaccatt accagcgaaa ccgggctggt caagatcagc aagacggtgt tgaacaccac
    11401 ccgtcactcc gcccctcaga tgccggatct ccgccggctt gacttgagcg gtccgtacaa
    11461 ggaatacgtt cgcagcttcc tcgccgaccc ggttacgggc ttcgactccg agcggccgtt
    11521 gaaactggtc gtggacgcct ccaacgggat ggccggccgc tggttgccga tcatcttcga
    11581 cgacgtcgag tggcttgaga tcacccccct caacttcgag cataacggcg agttcagcca
    11641 tgaccccgac ccccttgtgg aggacaacct caagccgctc caggaacgat tgctccgaat
    11701 caacgcggaa ctgggcgtgt gcttcgacgg cgatgccgat tgttgcgtct tcgttgacaa
    11761 caaagctcag cccatccgcg ccgatacgct cggggccctg ctggcgcgag aactgctcaa
    11821 gtccaatccc ggccagagtg tgatctatga ccttcggagc agccgcgcgc taccggagga
    11881 aatcaggaaa gccggcgggt tgcccaggcg tgaacgcgga aaccaagcct tcgtaaagaa
    11941 ggcgattgcc gatgccaaag gcctgtttgc ctgcgagttg aacgggcgct attactatcg
    12001 tgacaatttc tactgcgaat cggcaataat cacgctcgtc cacgtgctga acatcctgac
    12061 cgagacgggc aggccgctgg acgagctggt cgcctcactc aagcgctacg ccaggtcggg
    12121 cgaacggcgt ttccgcctgg acggcaagac ggatatcgtt gccgtcctgt ccgacaagta
    12181 caacaatgga agcatcaact atctcgacgg catcaccgtc atctttgagg attggtggtt
    12241 caatgcccgc cccgccgccg accggtcgtt tgttgaactc aacgttgagg ccatcaacga
    12301 ggccatgctg aaggccaagc tcgcggagct gtcgcagctc ctcggcgacc ctctggtcaa
    12361 cggagcttga gtgaggaacc cttggacgag cctcttgcag acgccggccg aagcgtcgaa
    12421 ccggacccgc gaggtgcagt accaacgatg acgcctgggg atgaacacgt tcactatctc
    12481 cgccttgcca acggaatcga cctggcggtt ctgccaaccg gtcgccggtc ggttacggcc
    12541 atcgagatgc gtttcctggg cggttacgcc tacgaacggc ccgaccggct tggcgtggcc
    12601 catatcctgg ccgagactat gaccaaggga acgcagcggc gggacggtcg ggctctcaac
    12661 gatgcctttg acgaaatcgg caccagctac gccgtctcgg cggcgaggga aacgttcgtc
    12721 atctcatgcg tatgcctgcc cgagttcgtg gcgcaggccg tggaactgct cgccgagatg
    12781 atcaacgaac ccagcttccc cggcgaggct tgcgaggtgg cggtcgaact cgcccggcaa
    12841 tcgctggccg ccctggagga cgatcctctc gaactggcca agaagtgcct ttgtcaacag
    12901 gcgtacagcc cccctctgaa ccgccacgtc tacggcgagc ctgccaccgt cgagcgcatc
    12961 ggtcgcgagg acgttgtcga gcactggcgg acgtacctga agcctgaccg tttgcttgtc
    13021 tcagtggccg gtgccgttga cccgagcgcc gtggcggacc ttttcgagcg tctttttccg
    13081 gcaacgggcc tcaacgaccc cgtggtgaac gcatctttgc cgctgtcatt tccgcttggg
    13141 ttccgtccgg gacagtcgca tcgccagaaa gatctcgagc aggaacaaat cggggtgtgt
    13201 tttcccggag cgtcggccgt ggacgaagat gaggccgtgg aacgcgtgat catcggcctt
    13261 ctgtcgggag gaatgagcag ccgactctgg cagtccatcc gggagaagca gggtctcgtc
    13321 tattgggtcg gggcctggtt tgaccggccc cgaacgggcg gaatgatcca tctgggctcg
    13381 tcctgcactc ctcaaaacct tgaggcgaca tcggaaatgc ttctcaagga agtgaatcgg
    13441 ctggccgacg acgtcaccga gtctgaagtc cagagagcca tcagcggcat attggcatcg
    13501 gcgcagaccc acggcgacgt aacccgggcc aaagcgacac gcatcgtcaa cgatctgttt
    13561 tacctcggcc ggcccatccc actggaggaa aagctgtctc gcatcagggc tgtcacaatc
    13621 ggcgacgtcc gacagtacct gcgacggcat cctcgcgagc aactgagcat cgtcacggtc
    13681 ggccccagat gtctgaaaga cttcaggtaa ggctgcgtcg gtcgcgcttt ttcgaggcaa
    13741 gaacatggcc catactcggt tctttcagga aacactagcg aacgggttga tcgtggccgt
    13801 cgaggtcatg cctgaaaccc gatcggcggc ttgcggcttt ctggtccgag ccggatcgcg
    13861 ggacgatccg ccgcgtctgg ccggcatgtc tcacttcctg gaacacatgt gcttcaaggg
    13921 aaatgagcgg ctaacctccg aggatctcaa catcggcttc gacgagatcg gtgccgacac
    13981 caacgcccag accagcaagg accagacctt ttactacagt tgggcccgct gcgaggacct
    14041 cggcaggcaa atcgagttgc tggccgagtt gatgagaccg tcgattccgg ccggagaatt
    14101 cgagatcgag aaggacgtca tcctcgagga aattgcgacc tacaaggatg acctgtccgc
    14161 cctggcctat gattctctgt acgaggagct ttgcccgggc caccccatct cgcgtcccgt
    14221 gctcggttat gaggagacga ttcgagtcat gacgccggaa gacatgcggg actatctcgg
    14281 ccgcaagtac gccgcgagca acatgattct gatcatcacg ggaaacgtcg atccggccga
    14341 ggtgatgggc ctggcgcgcc ggtattgtgg tagctggccg tccgtaataa acaaaggccg
    14401 tgatgggcac ccgcctgcgc tgaggacggg ggcaaccggc cggatcatcg accgtttcca
    14461 ccagcaagcg gttgcagtag cattcccctc ggtcccgacc actcacgccc tcgacgaaac
    14521 cgccgaggca gtggcggcca tcctcggcgg cagcaactcg cgcttcttct ggaatatcca
    14581 tcagaagggc ctggccactc gggtgagcgt catccgggag gagtacgccg atttcggggt
    14641 tatcattctg gtcgggctgt gcgaaccgga aaacgccgag agactcctgg aggccatgcg
    14701 tcgcgaggcc gagcaattga cccgagacgg accggagccc aaggaaatac gacgggtaaa
    14761 gaatcatcgc cgcacgatgc tgacaagtga atctgaagcc cccggttacc gcctcggaca
    14821 aattgccgat gatatcgact atcgcggcag cccccgcacg atggaagaga ggctggcgga
    14881 agtggatgcc gtctcggccg atacgatcgc cacgtatcta agagactacc ctattgtggg
    14941 cgaaggcttc gtcgccacaa tcggaccgcg gatgtggctt tcggcgaaat gaagcggcct
    15001 tgtcgagggt ccgggaagac gtatggaacc gaccacccct gatgaacacg atatcgcccc
    15061 gccaccacag ggcggcccgc cggactctcc gcccgacgcg gagggccacg cgggcctggc
    15121 agtaacccgg ccgccgatag catggatggc gccgggcatg ccatttgccc ggccaatcac
    15181 gatcaccgtc ctttggccgg atgcccccgt ctgtgttccc ggtagggcgc cggcagaaac
    15241 ggagcagccg ttgtcacggg cccgggcggt tatcgagatc atcctggcag tcccggtggc
    15301 cgccgtcgga gccgtggtgg ggtacagcgt gggcaagagc ttcgagcctg ccgatgaaag
    15361 atggctccga atcgccgact ccatgggctt gggcctcggt gccgtactgg cttgcatact
    15421 gctcgtgtgg cttggaaacc gcaagatcag cagcatcggg ttgaccatgc gccagtggca
    15481 ccttgacctg ggcatcggct tcgcctcgtt gcttgcgact tgggtcgtac tcctggcgtt
    15541 tgggggcgtc attgtcgtcc tgtttccgtc cgtcctccag gagcaatcgg cggcccaaaa
    15601 ggcgattgaa gccagctttc ccaagctttc ttggcaacag atgcttgctc tttgcctgtg
    15661 catcgcgatc tacgaagaaa tcgtctttcg cggtttcctg ctgacgcgtt tgcatgcact
    15721 ggttcgccga tggtggctgg ccgtggtgat cggagcggta ttgttcggcc tgccgcatca
    15781 ctaccaaggg tggtgggcag tgtgcggcat cactcttctg ggactgatca tgggctcgct
    15841 gtttgcctgg cgaaagagtc tcgtggcacc catcacgatg cacctgttcc acaatttcgc
    15901 cgtgttcatg ctgctggacc tggtctccgg catgcggaac tgacgggcga ggttatgagg
    15961 gtccggtgac ctgcggggtt gggtggccgg tcatctattc atctttgacg caccgaaagc
    16021 ccacggtcgc caaccgattc atgcccgggc cgaagagcgg caaagggtgg tgatcataga
    16081 ttcggcgcgg cccgcctctg acccaccaat gcccgccggg cggttggtac cagccgccgc
    16141 cccgcagaaa gacaacctgg tgacggccat tgtcggacag actggccgtg tactgccaca
    16201 cattaccaac aagatcctcc acaccgaaag gactggctcc gcggggatgt ttcaacacgg
    16261 cggaggtgcc cttggcgtca atgggacatc gcgcggcctg gggttcgtcg aacccccaag
    16321 gccatgggcg gccgtcttcg cctccggctg caaactgcca ttcttcctcc gtcggcaagc
    16381 gtttgccagc ccagctcgcg tatgccacgc aatcatcgta actcacgtaa accaccggat
    16441 gatcctcatg gccaacaggc ggtttcccat cgacgaaatg atgcaagaag tgagttgcgt
    16501 ctttggggtt gtaacccgta acgcggacaa actcggcata ctcggcgttc gtgacgggag
    16561 atcggtcgat ccagaagggc tcgatgtgca ccgtccgagg tttcgaggcc tcaccgggac
    16621 cgtactggtc caacgtgtga tagcacggga tccacgtggt tgccccgtgc ttgacgacgt
    16681 atgagaactc cccgcccgga acgcggatca ttccggccgg cggcgactca gggtcaaccg
    16741 gcgccgtcct ccccggcttg tcgatgcgga agtagcgaag tacgctttcc ggcaacacca
    16801 cctgatccag cagttggttg ttcgcatcga gcagtcgaac cacgattgcg tcgttggtat
    16861 gacgaccgaa tgcctggtag agatccaact ggactcgctc tgcggcaggc actcgcaggg
    16921 gattggtatc cggtcgtact cgattacacc agatctcgac cactgccggc agcgacattc
    16981 ggtctacggt gaccttgagc atctcgaatt ccagttcggc gtccaggact cgcggcagga
    17041 ccgcgataca ccctgcggaa aaatccccgt cgcccccctg gcgaccggct tcatagggct
    17101 cgatccgcac actcagaagg tcatgatctc cctcgcgccg gacgtcaacc gggcggtcgt
    17161 tccacaagtc gacgtagtgc acctcaggac gatgcggcaa tcgaaggagc acgccttgat
    17221 ggccgccagg gtcagtcccg cacagcgtgt agatcgtctt gcccgccgcc ggccagcgat
    17281 tgacccacac ttcacgatcc aggctgccga cgaaaggctc ccaattcaac gaggaaaaat
    17341 tggccctgtt ggtgcgcaga atgttgaccg cccgggccag gacaccgaaa tcctcctggc
    17401 aggaaggccg cttctcggga aacatcgtgt tgatctcgac gccatgcccg ttgaagaaac
    17461 tgagcacaaa atccctgcgc agcagtcggc cggcggcccc atcctggcat acccgcaatt
    17521 gcggctgatg gggcagtaga tactttttga agttgaagta tggcatttta tggacattgt
    17581 ggatccgtcc gagcaagttg gtctggctct gataccagcc gttgtcgcct tcggcatagg
    17641 ggagcagttc gcggccgctt tttcgggcca tctcgcggat ctcaggcgga gtcgtgtcca
    17701 tgatatccat caggacgccg tgcgccccga cggcgtgggc cgtgttcacg agttccctgt
    17761 aagaatccag atgtccctgc tgtgacggat cgcgatcggt ttcgctccag atgcagtggg
    17821 caatgatcgt ccgcgtaccc atggtctccg ccttgcgaac ctgctccgcc aatccttcga
    17881 ggccgccggg catatcgcgg aagtgctcga actgataacg ggcgtcccat cctgcccgcg
    17941 gccagaaagg ccagatcatg aaaacgtcgt aacccccgaa gagtctgcga gcgttgccaa
    18001 gggactcaag caaccgatac cgtcccgtgc gcgggtccat gacctcctgg tcccacgccc
    18061 agttcagcca gccaaccacg atatttcttg accaggccag atccggatgg ttgtactcgg
    18121 aataatcgta cttggaggga tcgacccggt atccgccacg ccgacgatag gtttcgctga
    18181 gggcctcaga ccggttaccg atcccgacgt cgagatgcaa ggtgtacgtc gccgatcgac
    18241 cgggtttcag aatcacttcg gtcccttgcc cttcgccgtg gtccctggtc agcaatgagc
    18301 aaacgtaagc tcgcccggtc gtggcaggca tcgtggcaaa gacatcacgg gaaaactcga
    18361 ccgggaccca cacgggatgc cttccgaccc cgccggccac gaactcggga ggacacagcg
    18421 cgaaatgatc gaggcaagcc tgctgcacct gctccggggc atcagtccag cgcgtaaacg
    18481 gcggcaagag gacgccgcgg atatcctgtg agcctgcatt actgatcacg atcgcgaagc
    18541 gtcgctgacc gtacgggcct gtatccaggc ttttgacaac acacgtcagg ttctcgattc
//
LOCUS       DAOTPW010000106        18498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3050_length_18498_cov_5.098466, whole genome shotgun sequence.
ACCESSION   DAOTPW010000106 DAOTPW010000000
VERSION     DAOTPW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18498
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3050_length_18498_cov_5.098466"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2398)
                     /locus_tag="P5316_11115"
     CDS             complement(<1..2398)
                     /locus_tag="P5316_11115"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRR85548.1"
                     /translation="MHRQLKPIYLIQMNHLDPIWRRCWDHRFECEGRTFASYADLQEA
                     IISDWIKTAERGKTAFVLEQTVSLRKYLERHPEHYGTLKRLAREKRCEVLAGGEVIPD
                     ANMPLGESLVRNLLYGILWVEKTLGIPVNIGCRNDGFGSSAQIPQIFRQCEIRWLPFL
                     SYRTPTGDYWRGLDGSTLYVRAPQARLLLGTSVKLAPCDACKGKGCEACQDRGFDQNV
                     RVELTSWLEDTGKEPFGLISLGGEETLPCMDLEKQVAERAVNANGIEYRFGIYQDLLP
                     HVAAELEAVDNPPPEHVAADPDIALSSAGCWVTRIKLKQRNRRAEHALLAAETLAAWA
                     WLRGEQYPTDLLADTWRSLVFTHFHDAITATHIDAAYAELMNLYDWLDADTQAFTARA
                     AHAITKRSAQNGQDRPATIFNTLSWARTAPVSVEIAGWPTEWATACDDQGPLPVYGVD
                     RRQDDTAVIHTLGRDVPLLGSRGIRLSPAAQPPAVETLEGDSIENERFRITSDEHGIT
                     SIVDKSTGDELIRPGMFYSGELILEHDYGDPWATRRADHHRERLSPLTRRSAVRRVPG
                     GCEIIFEGEHPGNPSDFEVVWLTWKQRVLLRDGLPYVEFVTQVDWDTYNRRIRIAFPT
                     TAHGDDGDYEIPYGTVRRGRYEPTYEANGVNGDWPAIHWAAPASEDTRAAIINRGECS
                     YRIEDGTVLVSLLRSPGSPWCLHEPQFYRMPLFDGMRDAGKHEFRLAMLPFKGPWQDS
                     GVTQAAWSYNAPLPTVADCTAAVPSMGLGLSAKGTMISAVKRAEDGDALIVRLYEYAG
                     R"
     gene            complement(2408..3112)
                     /locus_tag="P5316_11120"
     CDS             complement(2408..3112)
                     /locus_tag="P5316_11120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR85549.1"
                     /translation="MSRTNSQTTEDSSALTDAPGGLGRYAKDWMSDEQAARYVVSRRP
                     DRYKHYDIEQKLVGKWLSLCAPGATVLDFPCGTGRFNELVRQCGHRLIRADRSTAMLA
                     QARTHGPNDHSLGNICCDLAHPPFPDNSVDIMILWRIFHHLRSWDDRRTVLQQAARVA
                     RKYVIMSFYDRACLTYWAQVFAQKCFRTRPKLGGAIRTSDLLHLTASLGLSPVEIHHY
                     RRFISLNSAACFRLSQ"
     gene            3266..4339
                     /locus_tag="P5316_11125"
     CDS             3266..4339
                     /locus_tag="P5316_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRR85550.1"
                     /translation="MEQGHSRNTTAERDSADRYCRQVLFAPFGPEGQRRLLASRVLLV
                     GCGALGSTLANLIVRAGVGFLRIVDRDVVELDNLHRLVLFDEDDVREGLPKAEAARRK
                     LARINSGVTVEAVVDDVSHTNIVELAAGADLILDATDNFETRFLINDLAVKTGRPWVY
                     GACVGSTGLSMPIIPGETPCLRCVFEDPPPMEMSPTCDTAGILGPVVGMVANHQAMEA
                     IKILSGHKEAVDRRLLSFDAWTGRSSRLDVRKAFEQGDCPCCKQGRFEYLEGAFASRT
                     VRLCGRNAVQVYPSRAGAVDLPALAARLRPLARSEPKLNPLLLKVSVDDYEITVFADG
                     RALIKGTNKPEEARAVYARYIGA"
     gene            4567..6015
                     /locus_tag="P5316_11130"
     CDS             4567..6015
                     /locus_tag="P5316_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85551.1"
                     /translation="MRGITRRRFLEDSMLAAAAAAGGVAVGRGAFAAEPRRARKAGPN
                     DQLRIACIGFRGQGQNHIKAYLGMDDVTVATLCDVDSNLFANGVKMCEEKGKKVPKCE
                     QDLRRVLDDESIDAVSIATPNHWHSLAAIWAMQAGKDVYVEKPISHNVWEGRRLVEVA
                     RQLDRICQCGTQCRSMRGMIEAIEYVHKGGIGKVSMAHGLCYRDRGSIGIAGGEQPIP
                     PGIDYDLWLGPAPKVPLTRKQLHYDWHWFWAYGNGDLGNQGIHQMDIARWGLKKDRLA
                     DSVVCLGGRFGYKDDGETPNTQLTLLDYGDCQILFECRGLKTKQHKGAGVGVIFYGDE
                     GYVVVPKYEGGAAFDLNGKVVKEFGGGGDHHRNFINAVYSRKHTDLTADCLEGHLSSA
                     LCHLGNISYRLGTDQPFDSANKAFGDNQEAHETFARIQEHLKDNAVPVDGLKYHVGPK
                     LAFDVKAERFVGNEKANEMLTREYREPFVVPA"
     gene            complement(6559..8871)
                     /locus_tag="P5316_11135"
     CDS             complement(6559..8871)
                     /locus_tag="P5316_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRR85552.1"
                     /translation="MSRSIAILVVSSISGVAQAADVREAHADVLQPLPAADVKIGGYV
                     GHRLDRNLRGILMHKDENALLEPFRERGPKQSAWVGEHIGKWLSAASWMYAYSHDEQM
                     LAKLKRVAGGLVATQMPDGYLGTYADKDRWTGWDVWIHKYNMLGLLDYYDVTGDIAAI
                     AACRKMGDLLVNTFGPDKRDILKAGEHLGMASASILEPVLMLYRRTEEQRYLEFANYI
                     VEATERSDGPRIISTLLKDSDVQKVANAKAYEMLSDIIGMIDLHRISGDDRLLRAAMA
                     AGDDIIRHRLYITGGCTFGEHFRDEGYLPNAGHVAETCVTMSLMQFYHDLQGLTADVK
                     YADSAENLILNHLLACQRPDGERICYYTPLWGHKFYFDFLGCCISSGPRALAIIPSMY
                     YLRWRTSAVTATDAVGVYMIGESTLDTTLPKGAKAHVEQRTDHPFSGRVLITASDANR
                     KGYSLSVRIPTCAPKPTVTVGGQVLPFNPPASQWLIGALVGDSPITIEIDLHLTWKII
                     EGTGANTGLFALQYGPVVYAFDLDANHEVPGAMNCAFDMDLKSLAPQLIKKDDRWTAR
                     VHGHVRQADGSWKPAEITLLPFADAGVTGYFSVWLIDRARYNPNGISLFTQVHENTSR
                     PGTNRGSFADNSTATFTSTDNGEKRDQDWFEVDAGWHAKFNVIVFRHGKATPAGGWFD
                     TSKGKPKILLKSWHDYKEVAEIADYPATTATSPGTLTDGQAFRVVIPKDKREPAFRVR
                     VTGTPAAGNSPAQNSASCSEIQVFYDPTME"
     gene            complement(8970..9473)
                     /locus_tag="P5316_11140"
     CDS             complement(8970..9473)
                     /locus_tag="P5316_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85553.1"
                     /translation="MPVQFRRDELPADDNDVRQRVVWEANGSTKVGDVARAVYAGLWV
                     SVPGAVGLCLGLNLIKRALLAAEVLSKDNDYWRETWIPMLLFCFYGGLLGAGIAWRVT
                     SAVSLGGPAIWLAAGASLILVLLAGIGASTWFVFPGNVPSTCWIGLGLLVGAAVVGVH
                     AVNAWND"
     gene            complement(9543..9974)
                     /locus_tag="P5316_11145"
     CDS             complement(9543..9974)
                     /locus_tag="P5316_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85554.1"
                     /translation="MKAVLVLIVLAVVVIGLVYKFGGYASFDPTAQGREAKGKIQPGM
                     TVKQVVDIAGPNGKFHNISVLKQKIRGQDVVKTVRGPAMSLNPDQLNARIQRNELPNG
                     FTIEYVFSQQAAFEVIFDSTGKVTGIENVTTMADLLNSRED"
     gene            complement(10169..12001)
                     /gene="typA"
                     /locus_tag="P5316_11150"
     CDS             complement(10169..12001)
                     /gene="typA"
                     /locus_tag="P5316_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRR85555.1"
                     /translation="MKRTDIRNVAIIAHVDHGKTTLVDQMLRQCGQFRDAELKGERIL
                     DSNDLERERGITILAKNIAIRYKDTKINLIDTPGHADFGGEVERVLKMADGALLLIDA
                     FEGPMPQTRFVLRKAFEYDLRPLVVVSKMDRPDARPHDVLHETLDLFLELGADDETAD
                     FPIIYTSAAEGWASTSPDEHPDNIYALFEMILKQVPPPEVDIDAPLQMLITTLDYSDY
                     VGRIGIGRVFRGRLKVGQPVTVICRNGEPKTEKIGEIHAFDGLGRRAVDEVTAGDICA
                     IVGLESVDIGNTIADPEHPEPLAVIRIDEPTLHMSFRVNDSPFSGRSGKFLTSRHLRD
                     RLEKELQSNVSLRVEPGCTPEEFHVSGRGLLHLSVLIENMRREGYELAVGKPKVIYHE
                     QNGRRTEPVELCVIEVPPKYVGAVMELMGGRRGICVRMETRGEYTHIEFTVPARGLIG
                     ARNRLLTATNGTAIMHHNFHQYEDFRGSIAGRTNGSLIASETGTVTAYALDNLADRGT
                     FFVKPTDQVYAGQIVGEHCKDKDIEVNVCRAKRMTNIRAASADKTVVLKPPREMTLEM
                     ALEFIEEDELVEATPDAVRIRKRLLDSNARKQAARREAAGVSEA"
     gene            12667..12924
                     /locus_tag="P5316_11155"
     CDS             12667..12924
                     /locus_tag="P5316_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRR85556.1"
                     /translation="MTDHRRSIVGAHEANVHFYELLKRVEKGEEVTITRHGSPVARLV
                     PVKQSVASEERTASIRRWQKSSKGITVGRLKVRDLVNEGRP"
     gene            13072..14994
                     /gene="mutL"
                     /locus_tag="P5316_11160"
     CDS             13072..14994
                     /gene="mutL"
                     /locus_tag="P5316_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRR85557.1"
                     /translation="MPQIHVLPDLLVNKIAAGEVIERPASVVKELVENSLDAGARRIE
                     ITIEQGGRKLIRVADDGGGMDVDDLALAVQPHATSKITREEDLFGIRTMGFRGEALPS
                     IGAVSQLRIVSRQPNSDAAHEIRVTADRIEPVTAASGPVGTTVEIRELFFNVPARHKF
                     LRTPQTEMGHITEQVARIALVYPQVEFRLIHNGRPVQQLRPAGSMRERIADFFGKEMS
                     DSLLEVVRDERGLKIHGYVGRPSDSRSSARWQYFFLNGRYIRDRFLSHAVREAYRGLM
                     EAHRYPVVFLALEVPPDSVDVNVHPTKIEVRWRDSNMLYSQVLSAIRDRFLNTDLTPG
                     LMAVRSPQIADPTGEFSGDLIEARESAVAGPDSEAERRHREIRESIADFFKRNKPPVP
                     SGFGEGRPVCTAYSPSQPIEAYQPARMVDSDAALESFEPPSLLPPASVRAGESEIDRI
                     DLGTVLQIHRSYLVTETSDGLVIIDQHALHERILFEQLSQEITRGPLESQRLLLPDMI
                     DVAPDQVAVIEAHADTLAKLGFEMTSYGPSTIAVHAVPSILKPERVTAFVRDMLDRLA
                     VRSGPAASEMLINDLLSMMACKAAVKAGDPLDPAEIRSLLAQRHHIERSSNCPHGRPT
                     SLRFTLRDLERQFKRV"
     gene            15358..16935
                     /locus_tag="P5316_11165"
     CDS             15358..16935
                     /locus_tag="P5316_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRR85558.1"
                     /translation="MLQRPWVRIVFAGFVLAAFAMLAAGADAPAAERLPNIVFFLADD
                     MGYGDPRCLNPDSKIPTPHMDRLAAEGMVFRDAHSPSAVCSPTRYGILTGRYCWRTEL
                     KRGVLVPWDRPLIEPGRLTMPAMLRQHGYVTACIGKWHLGWDWPTRDGRPVPDSLRHA
                     RSDGNLAMEDYEVIDLTRVIDSGPLTRGFDSYFGMDVPNYPPYCFIDDDRTIGIPTQL
                     KPGRMFGDPGPMVPGWRLEPILPTLGARAVTFIDRHAAQHRDKPFFLYFASTAPHTPI
                     VPDDPFVGKSQAGPCGDLVYQVDWTLGQILAALDRNSMRENTVVVMTSDNGSPAIAGD
                     PFKHGPDLSSRSASIAKFGHDPNAPWRGMKADIWEGGHRVPLFVRWPGRIAPGSTCGQ
                     TVCLTDFMATFAALVGHRLPNDAAEDSYDLSPLLLSKEGSEAIREATVHHSVDGVFAI
                     RQGKWKLIMSLGSGGWSKPTGGEPEPGGPKGQLYDMQADPAETKNLWTDHPDVVKRLT
                     LLLEKYQRDGRSVPHRN"
     gene            complement(17009..17482)
                     /locus_tag="P5316_11170"
     CDS             complement(17009..17482)
                     /locus_tag="P5316_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85559.1"
                     /translation="MSSLLAVDVGLRTGLALYGRDGRLRWYRSRNFGNATRLRGGVQA
                     VLRETPDLEWLVLEGGGPLADIWTHEAQRRGVLLRTISAEDWRRSLLYPRQHRNGPQA
                     KHSADIVARRVIAWSQARRPTSLRHDAAEAILIGLWGLVSIGWLKTLPTELHHET"
     gene            complement(17534..>18498)
                     /locus_tag="P5316_11175"
     CDS             complement(17534..>18498)
                     /locus_tag="P5316_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR85560.1"
                     /translation="RQARRRLHRLREAVDALARGDLATCIDHPPDDDFLKLSDSVERV
                     LNQLRENCREHERLRRQLTHSEKLALIGELAATVAHEVNNPLDGLQNSIRILRRGVDN
                     PDQVRQLLDLMENGLYRIEMIMRRLLTMSRNEPLSPEPTRIETIVDDALMFAQPKLNR
                     RRVELIRDLPESLPFAMADRLQMAQVLINLMINAADAMPNGGKLTIRCRSGDYGRTLL
                     LDVIDTGSGIAPEHLPHIFEPFYTTKSKGGGTGLGLAVVSRIVRAHGGTVEIQSEVGK
                     GTRFRIILPAARSLEQSESTQPAAGIPTLHQLMQPAEASRAKNQ"
BASE COUNT         3307 a         5400 c         6136 g         3655 t
ORIGIN      
        1 cgcgcccggc gtattcgtag agccggacga tcagggcgtc gccgtcttcg gctcgcttga
       61 ccgcgctgat catcgttccc ttggctgaca gaccgagtcc catcgagggc accgccgcag
      121 tgcagtctgc cacggtcgga agtggggcgt tgtaactcca agcggcctgc gtgacgccgc
      181 tgtcctgcca tggtcccttg aaaggcaaca tcgccagccg gaattcgtgc tttccggcgt
      241 cgcgcatccc gtcgaacagc ggcatgcgat agaactgcgg ctcgtgcagg caccagggcg
      301 agcccgggct gcgcagcagc gacaccagca ccgtgccgtc ttcgattcga taactgcact
      361 cgccccggtt gatgatcgcc gcgcgggtgt cctcgctggc aggtgcggcc cagtgaatcg
      421 cgggccagtc gccgttgaca ccattggcct cgtaggtcgg ttcgtaccga cctcgccgga
      481 cggtgccgta cggaatctcg taatcaccgt cgtcaccatg agcggtggta ggaaaggcga
      541 tgcgaatccg gcggttgtag gtatcccaat cgacttgcgt gacaaactcg acgtagggta
      601 acccatcgcg cagaagcacg cgctgcttcc aggtgagcca cacaacttcg aagtcgctgg
      661 gattgccggg atgctcgccc tcaaagatga tctcgcaacc accggggacc cgccgtaccg
      721 cggatcgtcg ggtcagcggg ctgagtcgct cgcggtgatg atcggctcga cgcgtggccc
      781 aggggtcgcc gtagtcgtgt tcgaggatca actcgccgga gtagaacatc ccgggcctga
      841 tgagttcatc gccggttgat ttgtcgacga tgctggtgat tccgtgttcg tcggatgtga
      901 ttcgaaatcg ctcattctcg atcgagtcgc cttcgagagt ctcgaccgcg ggtggctggg
      961 cagccggaga caggcgaatg ccgcgagacc ccagcaaggg gacatcacga ccgagggtgt
     1021 ggatcacggc cgtgtcgtcc tgccgccggt caacgccgta taccggcaac gggccctggt
     1081 cgtcacaagc ggttgcccat tccgtcggcc agccggcgat ttcgacgctt accggggcgg
     1141 tgcgtgccca actgagcgta ttgaagatgg ttgccggtcg gtcctggccg ttttgggccg
     1201 atcgcttggt tatcgcatga gcggcacggg cggtgaacgc ctgggtgtcg gcatcgagcc
     1261 agtcgtagag gttcatcaac tcggcgtagg cggcatcgat gtgcgttgcg gtgatcgcgt
     1321 cgtgaaagtg tgtgaaaacc agcgaacgcc acgtgtcagc gagcaggtcg gtcgggtact
     1381 gttcgccgcg cagccacgcc caggccgcca gggtctcggc ggccagaagg gcgtgttcgg
     1441 cccggcgatt tcgctgtttg agcttgatgc gcgtcaccca gcagccggcc gacgacaggg
     1501 caatgtcggg atcagcggct acgtgctcgg gcggaggatt gtcgacggcc tccagctcag
     1561 ccgcgacgtg cggcagcagg tcctggtaga taccgaaacg atactcgatg ccgttcgcat
     1621 ttacggcccg ctcagccacc tgcttttcaa ggtccatgca tggcagcgtt tcctcgccgc
     1681 ccaagctgat cagaccgaat ggttccttgc cggtgtcttc gagccaactg gtcaactcca
     1741 cgcggacgtt ctgatcgaaa ccgcggtcct ggcaggcctc gcagcccttg cccttgcagg
     1801 cgtcgcacgg tgccagtttg acgcttgtgc cgagcaatag cctggcctgc ggtgcccgca
     1861 catacagcgt cgaaccatcc agacctcgcc agtagtcgcc ggtcggagtc ctgtaactca
     1921 ggaagggcaa ccagcggatt tcgcattgac ggaagatctg cgggatctga gccgatgagc
     1981 cgaaaccgtc attccggcag ccgatgttga ccgggatgcc caacgtcttt tccacccaca
     2041 ggatgccgta gagaagattg cggacaagcg actcgccgag gggcatgttg gcgtcgggaa
     2101 tcacctcgcc gccggccagg acttcacatc gtttttcacg ggccagccgt ttgagcgtgc
     2161 cgtagtgttc ggggtgccgc tccaggtact tgcgaagcga gacggtctgc tcgagcacga
     2221 atgccgtctt tcctcgttcg gccgtcttga tccagtcgct gataattgct tcttgcagat
     2281 cggcgtaact ggcgaaggtc cgaccctcgc actcgaaccg gtggtcccag cagcgtcgcc
     2341 agatcgggtc caagtggttc atctggatga ggtagatcgg cttgagctgt ctgtgcatgt
     2401 ctcgatctca ctgtgacagc cggaagcatg cagccgagtt gagagagatg aaccgacggt
     2461 agtggtggat ctcaaccggg ctcaggccga ggcttgccgt gaggtggagc agatccgagg
     2521 tccggatcgc accgccgagc ttcgggcggg tgcgaaagca tttctgggcg aaaacctggg
     2581 cccagtaggt caggcaggcc cggtcgtaga aggacatgat gacgtacttt cgagccacgc
     2641 gggcggcctg ctggagaaca gtacggcgat cgtcccacga gcgaaggtga tgaaagatcc
     2701 gccagaggat catgatgtcc acactgttgt cagggaacgg aggatgcgcc agatcgcagc
     2761 agatattgcc caaggagtgg tcgttggggc cgtgggtccg ggcctgagca agcatggccg
     2821 tcgagcggtc ggcccgaatc agccggtggc cgcattggcg gaccaactca ttgaatcgcc
     2881 ccgtcccgca ggggaagtcc agaacggtgg ccccgggggc gcacagggag agccactttc
     2941 cgaccagctt ctgctcgatg tcatagtgct tgtagcggtc cggacggcgg gagaccacat
     3001 agcgggctgc ctgctcgtcg gacatccagt ccttggcata tcggccgagg ccgccggggg
     3061 catcggtgag ggccgatgaa tcttctgttg tctggctgtt ggttcggctc atggtcatgg
     3121 tccgccccga cgggcccttc tggcaggcca attggggaag aggtattgta acacaagggc
     3181 aggcggtgtg cattgggata cgatagcagt tttggagtcg cggaatcgga gcgctgctca
     3241 tgggcagtgg tatgcgagac gcgtgatgga gcaaggccac agccgcaata cgacggcgga
     3301 gagagattcg gctgatcggt attgccggca agtattgttc gcgccttttg gcccggaagg
     3361 gcagcggcgg ctgctggctt cgcgcgtgct gctggtgggg tgcggggcgc tgggcagcac
     3421 gctggccaat ctcatcgtcc gcgccggggt tggtttcctg cgaatcgtcg accgtgacgt
     3481 cgtcgagctg gataatctgc atcgcttggt gctgtttgat gaggacgatg ttcgggaagg
     3541 gttgcccaag gctgaggcgg cgcgacgcaa actggcgcgg atcaacagtg gggtgacggt
     3601 tgaggcggta gtcgatgatg tcagtcacac gaatatcgtc gagctggccg cgggtgccga
     3661 tctaattctc gatgcgacgg acaactttga aacgcgtttt ctgatcaatg atctggcggt
     3721 caagaccggc cggccgtggg tgtatggggc gtgtgtcggc tcgacgggcc tgtcgatgcc
     3781 gatcattccc ggcgagacgc cctgtctgcg atgcgtgttt gaagatccgc cgccgatgga
     3841 gatgagcccg acgtgcgata cggccggcat cctcgggccg gtcgtcggca tggtggccaa
     3901 ccatcaggcg atggaagcga tcaagatcct cagcgggcat aaggaagcgg tggaccggcg
     3961 cctgctgagc tttgatgcgt ggacggggcg gtcgtctcgg ctggatgtgc ggaaggcatt
     4021 tgagcagggc gattgtccat gctgcaaaca ggggcgattc gaatacctgg agggggcgtt
     4081 cgcgagtcgg acagtgagac tctgcgggcg gaacgcggtg caggtttatc cgagccgggc
     4141 gggggcggtc gatttgcctg cccttgccgc gaggttgcgg ccgttggcac gatccgagcc
     4201 caagctcaac ccgctgctgc tcaaggtgag cgtcgatgac tatgagatca cggtgttcgc
     4261 cgacgggcgg gcgctgatca aaggaacgaa taagccggaa gaggcccggg cggtgtacgc
     4321 gagatacatt ggggcgtgaa cgatcgccgg ttgtctgagg cgggaggaac cactggcgga
     4381 cgagccgtca gcggcacccg gactgtaaga agacatgtcc acgcggagcc gtggacatcg
     4441 cgcccggcgc ggcccaaggc agcggttttg gcttgtcggc gacttctcag tacactgtgc
     4501 aagtctactc aggacattga gtgaattgtg aacggcggtt ggaaagggcc ttgtgaggag
     4561 cacacgatga gaggtattac gcgcagacgt tttctggaag attcgatgct tgcggcggcg
     4621 gctgcggcgg gcggtgtggc cgtgggtcgt ggggcctttg ccgccgagcc aagacgggcg
     4681 aggaaggctg ggccgaacga ccagttgcgg attgcctgca tcggcttccg cggccaggga
     4741 cagaatcaca tcaaggctta ccttgggatg gatgacgtaa ctgtcgccac cttatgcgat
     4801 gttgactcca acctgtttgc caacggtgtg aagatgtgcg aggagaaggg caagaaggtc
     4861 cccaagtgcg agcaggatct tcgccgcgtt ctcgacgacg agtcgataga tgccgtgtcg
     4921 atcgccacgc cgaaccactg gcattcgctg gccgcgatct gggcgatgca ggcgggcaag
     4981 gatgtgtacg tggaaaagcc gatcagccac aacgtctggg aagggcggcg gctggttgag
     5041 gtcgcccggc agcttgatcg tatctgccag tgcgggacac agtgcaggtc gatgagaggg
     5101 atgattgagg ctattgagta cgtgcataag ggtggaatcg gcaaggtctc gatggcacac
     5161 ggcctgtgct accgcgatcg cggcagtatc gggattgccg gcggcgaaca gccgatcccg
     5221 cctgggatcg attacgacct ctggctcggc ccggcgccga aggtgccgct gacgcgcaag
     5281 caactgcact acgactggca ctggttctgg gcttatggca acggtgacct cggcaaccag
     5341 gggattcacc agatggacat cgcccggtgg gggttgaaaa aggaccggct ggccgacagc
     5401 gtcgtgtgtc tgggcgggcg attcggctac aaggacgacg gcgagacgcc gaacacgcaa
     5461 ctgacgctgc tggactatgg ggactgccag atcctttttg aatgtcgcgg tctgaaaacg
     5521 aagcaacaca aaggagcggg cgtgggagtg atcttctacg gagacgaggg gtacgtggtg
     5581 gtgcccaagt acgagggcgg ggcggcgttt gacctgaacg gcaaggtggt taaggagttc
     5641 gggggcgggg gcgatcacca tcgcaatttc atcaacgcgg tttatagccg taagcatact
     5701 gatctcaccg cggactgcct cgaagggcat ctgtcgagtg cgctgtgcca cctggggaac
     5761 atttcctatc ggctgggcac cgaccagccg tttgactcgg cgaacaaggc gttcggcgac
     5821 aaccaggagg cccacgagac gttcgcgagg atccaggagc atctgaagga caacgccgtg
     5881 ccggtcgacg ggttgaagta ccacgtcgga ccgaagctgg cgtttgacgt gaaggccgaa
     5941 cggttcgtcg gaaacgagaa ggccaacgag atgctgacgc gcgagtatcg cgagccgttc
     6001 gtcgtgccgg cgtagcctca acgccgccag gaggcattga ggtattgcag gttgcagatc
     6061 gggaaagcag gggctttcat tgcctcacgc cttgctgcac ggcccgatgc ttcccgagtc
     6121 tgggcatctt ccagaagacg ccacggtcgg cttggctgtc cgtgccctgt cctgagcagg
     6181 aagacactgg cgggcgagtc gctggtggtg cccggcgtct tgcggttgcc gttcgacggg
     6241 tttttcagcg taatcgatca tcgcgctccc atccttcgct gctgcgaagg gtgggccacc
     6301 cgactggggc ctttcgcgtg gaggattcct atcctcatcc tcatcaaacg cggaacggat
     6361 tccgccgcgc gaggcggccc ggaagggatt ccgggccgag cagcccacgc cgcacacgct
     6421 aaacacgtaa tcggggcacg taggctgtca gtagcccgtt tctattccag cgtcggcctt
     6481 gtcctgaaca agaggacact ggcgggcaag ccgccagtgg cacccgccca gagtctcatg
     6541 aacccgactt cggtagcgtc attccattgt cggatcgtag aagacctgga tctcgctgca
     6601 actggcggag ttctgggcgg ggctgttgcc ggcggcgggg gtgccggtga ctcggacgcg
     6661 gaaggccggt tcgcgcttgt ccttggggat gaccactcgg aaggcctggc cgtcggtgag
     6721 ggtgccgggg ctggtggcag ttgtggccgg gtaatcggcg atctcggcca cttccttgta
     6781 gtcgtgccag ctcttgagca ggatcttcgg cttgcccttg ctggtgtcga accatccgcc
     6841 ggccggcgtg gccttgccgt ggcggaagac gatgacgttg aacttggcgt gccagccggc
     6901 atcgacctcg aaccagtcct ggtcacgctt ctcgccgttg tcggtgctgg tgaaggtggc
     6961 cgtcgagttg tcggcgaacg agccgcggtt ggtccccggc cgtgaggtgt tttcgtggac
     7021 ctgggtgaac agcgagatgc cgttggggtt atagcgggcg cggtcgatga gccagacgct
     7081 gaaatacccg gtcacaccgg cgtcggcaaa ggggagcagg gtgatctcgg ccggtttcca
     7141 cgatccgtcg gcctgccgga cgtggccgtg gactctggcc gtccatcgat cgtctttttt
     7201 gatcaactgc ggggcgagcg acttgaggtc catgtcgaac gcgcagttca tggctcccgg
     7261 gacctcgtgg ttggcatcca gatcgaacgc atacacaacc gggccgtact gcagggcaaa
     7321 gagaccggtg ttggcgccgg tgccctcgat gatcttccag gtcaggtgaa ggtcgatctc
     7381 gatggtgatg ggactgtcac ctaccaacgc accgatcagc cactgtgaag ccggtggatt
     7441 gaatggcaag acctggccgc ccactgtaac agtcggtttc ggggcgcagg ttggtatgcg
     7501 tacggacaga gaatatccct tgcggtttgc atcggatgcc gtgatgagta ccctacccga
     7561 gaaaggatgg tccgtcctct gctcgacgtg ggctttcgct cccttgggca gggtggtatc
     7621 cagtgtactc tctccgatca tgtacacgcc aaccgcgtct gttgctgtca ccgccgaggt
     7681 tctccatcga aggtagtaca tcgacgggat gatcgccagg gcccgcgggc cgctggagat
     7741 gcagcagccg aggaagtcga agtagaactt gtggccccac agcggcgtat agtagcagat
     7801 gcgttcgccg tcgggccgct gacaggcgag caggtgattg aggatcagat tctcggccga
     7861 atcggcgtat ttcacgtcgg cggtcaggcc ctgcagatcg tgatagaact gcatcaggct
     7921 catggtcacg caggtttcgg ccacgtggcc cgcgttgggc aggtatccct cgtctctgaa
     7981 atgctcgcca aaagtgcagc cgccggtgat gtagagacgg tgtcggataa tgtcgtcgcc
     8041 ggccgccatc gcggcccgca gtagccggtc atcgccgctg atgcggtgca aatcgatcat
     8101 tccgatgatg tccgacagca tctcgtaggc cttggcgttg gcgacctttt gaacgtcgct
     8161 gtccttaagg agagtgctga tgatccttgg tccgtcggat cgctcagtgg cctcaacgat
     8221 gtaattggcg aattccagat accgctgttc ttccgttcgg cggtagagca tcaataccgg
     8281 ctccaggatg ctggccgagg ccatgcccaa gtgctcaccg gccttgagga tgtcccgttt
     8341 gtcggggcca aaagtgttga cgagcagatc gcccatcttt cggcatgccg cgatcgcggc
     8401 gatgtcgccg gtcacgtcgt aatagtcgag caagcccagc atattgtact tgtgaatcca
     8461 gacgtcccag cccgtccaac ggtctttgtc ggcataggtg ccaagatagc cgtcgggcat
     8521 ctgggtcgcg accaggccgc cggccacgcg tttgagcttg gcgagcatct gctcatcatg
     8581 cgagtaagcg tacatccatg acgcggccga gagccatttg ccgatgtgct cgccgaccca
     8641 ggcgctctgc ttgggaccgc gctcgcggaa cggttccagc agggcgttct catccttgtg
     8701 catcaagatg ccgcgaagat tgcggtcgag acgatgaccg acgtaaccgc cgatcttgac
     8761 gtccgccgcg gggagcggct gcaggacgtc cgcatgcgcc tcgcggacgt cggcggcttg
     8821 ggcaactccc gagatactcg acacgacgag aatcgcaatg gatcttgaca cgatggaagc
     8881 tcctaccttg tggtttggca gaccgtggct ttcaagctcg gttgagtctt ggctccagag
     8941 gggcgtggta agctcttgga ggccagatgt caatcattcc aggcgttgac agcgtggacg
     9001 ccgacgaccg cggcaccgac cagcaggccc aggccaatcc agcaggtcga aggaacgttg
     9061 ccggggaaca caaaccaggt cgacgcgcct atcccggcaa ggagaaccag tatgaggctt
     9121 gcaccggcgg cgagccatat cgccgggccg cccaggctca ccgcagaggt gactcgccag
     9181 gcgatgcctg ctcccagcag gccgccgtag aagcagaaca gcagcatggg tatccatgtc
     9241 tcccgccagt agtcgttgtc cttcgaaagc acctcagcgg ccagcagggc ccgttttatc
     9301 aggttgaggc cgaggcacag gcccacggca ccggggacgg agacccagag cccggcatat
     9361 acggctctcg caacatcgcc aacctttgtg gacccgtttg cttcccagac aaccctctgt
     9421 cgcacgtcgt tgtcgtcagc gggcagttca tcacgtcgga attgaacggg catggcaaga
     9481 cctccgacct gacggccgcc aggtccgtgg acctcgcgtc cggctttcct ccggcctttt
     9541 ggtcaatcct cccgactgtt cagcaggtcg gccatggttg tcacattctc gatgcccgtg
     9601 accttcccgg tgctgtcgaa gatgacctcg aaggccgcct gctgggagaa gacatactcg
     9661 atggtgaagc cgttcggcag ctcgttacgc tggatcctcg cgttcaactg gtcggggttc
     9721 agactcatcg ccggccccct gacggttttc accacgtcct gcccgcgaat cttctgtttc
     9781 agcacgctga tgttgtggaa cttgccgttc ggtccggcaa tgtcgaccac ctgcttgacg
     9841 gtcatgccgg gctggatttt gcctttggct tcccggccct gagccgttgg atcgaaggat
     9901 gcgtatcctc cgaacttgta aaccagtccg atgacgacga ccgccaggac aatgagcacc
     9961 aagacagctt tcatgggttt tcttcctttg ggttttgatt ctccaggcca atctcttctc
    10021 cagtgatgag atggtgcggg attctaacac ctcgtcggac aggatgcaac aggaagattg
    10081 cagtttcagc agccacgctt ctgaggcgaa gagcagggaa tcttagcaag gggtggggcc
    10141 gcataactcg gcggcattgt gttgttcgtc aggcttcgga gacgccggcc gcctcgcgtc
    10201 gggcggcctg ctttcgggcg ttgctgtcaa gcaagcgctt gcggatacga acggcatctg
    10261 gagtggcctc cacgagctcg tcctcctcga taaactcaag ggccatctcc aaggtcattt
    10321 cccgcggcgg tttgagcacg acggtcttgt cggccgaggc cgcccggatg ttggtcattc
    10381 tcttggcccg gcagacgttc acctcgatgt ccttgtcctt gcagtgctcg ccgacgatct
    10441 ggcccgcgta gacctggtca gtcggcttca cgaagaacgt gccccggtcg gcgaggttgt
    10501 ctagggcata agccgtgacg gttccggtct cgctggcaat cagggagccg ttcgttctac
    10561 cggcgatgct gccgcggaaa tcctcatatt ggtggaagtt gtggtgcatg atggccgtgc
    10621 cgttggtggc ggtgagcagg cggttgcgtg ctccgatcag gccgcgggcg ggaacagtga
    10681 actcgatgtg tgtgtactcg ccgcgcgttt ccatcctgac gcatatgcct cttcggccgc
    10741 ccatcagttc catgaccgcg ccgacgtatt tgggcgggac ttcgatcacg cacagctcaa
    10801 cgggctcggt tctcctgccg ttctgctcgt ggtagatgac cttgggcttg ccgaccgcca
    10861 gctcatagcc ttcgcgacgc atgttctcga taagcacgga aagatgcagc aggccccgcc
    10921 cggagacatg gaattcctcg ggtgtgcaac cgggttcgac gcgcagcgac acgttgctct
    10981 gcagttcctt ctccagtcga tcgcggaggt ggcggctggt gaggaatttt cccgaccggc
    11041 cggaaaaagg cgagtcgttg acccggaagc tcatgtgcag ggtcggctca tcgatacgga
    11101 tgacggccag cggctcggga tgctcggggt cggcgatggt gttgccgata tccactgatt
    11161 ccaggccgac gatggcacag atgtctccgg cggtgacctc gtccacggcc cgtcgcccga
    11221 ggccgtcgaa ggcatggatt tcgccgatct tctcggtctt gggttccccg tttcggcaga
    11281 tgaccgtgac cggctgtccg accttcaggc ggccacggaa gacgcggccg atgccgattc
    11341 gcccgacgta gtcgctgtag tccaacgtgg tgatcagcat ctgaagggga gcatcgatgt
    11401 cgacttcggg aggcgggacc tgtttgagga tcatttcaaa cagggcatag atgttgtccg
    11461 ggtgttcgtc cggcgaggtc gaggcccacc cctcggcggc cgaggtgtag atgatcggga
    11521 agtcggccgt ctcgtcatcg gctccgagtt ccagaaagag gtcaagggtc tcgtggagga
    11581 catcgtgcgg gcgggcgtcc ggccggtcca ttttgctgac gaccaccaac ggcctgaggt
    11641 catactcgaa cgccttgcgg agcacaaaac gcgtctgtgg catcgggcct tcaaaggcat
    11701 cgatcagcaa cagcgctccg tcggccattt tcaggacccg ctcgacctcg ccgccgaaat
    11761 cggcgtggcc gggggtgtcg atcaggttga tcttggtgtc cttgtagcgg atggcgatgt
    11821 tcttggcgag gatggtgatg ccccgctcgc gctccaggtc gttggagtcg aggatgcgct
    11881 cgcccttcag ctcggcgtcg cggaactgtc cgcactgccg gagcatctga tcgacgaggg
    11941 tggtcttgcc gtggtccacg tgggcgatga tcgccacgtt acgaatgtcg gttcgcttca
    12001 ctgtcacttc ctacggccgc ccctcttatg atgggtcgta acccagccca atacgcccca
    12061 aggaagtcat accaaacaac ccgaacagcc gtcggtgtca gaagccgagg cggagggcgg
    12121 tcgcccggac ggtttgaccc tgctgcaggc ggtcattata ccgtcgacgt gccgaagttc
    12181 catgcctcgg agatcggggg cacagcgccc gtgctgtccg ggtccgtgac agaggccgca
    12241 gccctcgttg cggttggtcc ggccggcatg gaagtgagcc ggcagcagac cgcttgtacg
    12301 tgttcagcgt gcaagacagc gggtcaggca cttttgccgt cttcttgtcg ccgacgagtc
    12361 ccgccggact gagccaacct ctccaaattg caccgcgaac cgcatttggg caaaaagagc
    12421 cagaccccag cacgctgaac acgtacggcc gtacaacggc cggcctgtca gcccgccctt
    12481 ggcggggaac cccctgaaga ggattgatca cctgtcgtat gccgtctggc gcacctcgtc
    12541 ggttctgtca cgccccatgc agatgggaag tgtctgaagc tgcagcgtga gtccgatagc
    12601 cgagttgacg tggctcgagg catcaggtgg acgagttgct ggtggtttct ccaccagagt
    12661 gcgcgcatga cagatcacag gaggagcatc gtcggggcac atgaggccaa tgtacatttc
    12721 tacgagttgc tcaaacgcgt cgagaagggc gaggaggtca cgatcacgcg tcacggctct
    12781 cctgtagcca ggctggtccc ggtcaagcag tccgtggcgt cagaagaacg tacggcgagc
    12841 attcgccgat ggcagaaatc ctccaagggt atcacggtgg gcaggctgaa ggttcgagac
    12901 ctcgtcaacg aggggcgtcc gtgagttggt cgtgccgctt ttggtgctgc caacgttgga
    12961 tgattctcgg ggcggcgttc ttatccgtgg tttgaacgta tccccggcag ggcgtatagt
    13021 agtaccacgt gtcgccgatt ctcttgactc gactcgcgaa cggatgcgcc tatgccgcag
    13081 atacacgttc tgcctgactt gctggtgaac aagatcgccg ccggcgaggt gatcgagcgt
    13141 ccggccagtg tggtgaagga actggtcgag aacagcttgg atgccggggc caggcggatc
    13201 gagattacta tcgagcaggg ggggcggaag ctcatccggg tggccgacga cggcgggggg
    13261 atggatgtcg acgatctggc cctggccgtc cagccgcacg cgacctcgaa aatcacccgc
    13321 gaagaggacc tgttcggcat tcggaccatg ggtttccggg gcgaagctct gccaagtatc
    13381 ggggcggtgt cgcagttgcg gattgtgtcc cggcagccga attcggacgc cgcgcatgag
    13441 atccgggtga ccgccgaccg aatcgagccg gtgacggccg cctcgggccc cgtgggcacg
    13501 acggtcgaaa tccgcgagtt gttcttcaac gtgcccgccc ggcataagtt tctccggacg
    13561 ccacagaccg agatgggtca tatcaccgag caagtggcgc ggattgccct cgtgtaccct
    13621 caggtggagt ttcgcctgat ccacaacgga cggccggtgc aacaactgcg tccggcaggg
    13681 tcgatgcgcg agcggattgc cgatttcttc ggcaaggaga tgtccgacag tctgctcgaa
    13741 gtggttcgcg acgagcgagg cttgaagatc cacggctacg tgggccggcc atcggacagt
    13801 cggtcgagtg ctcgttggca gtatttcttc ctgaacggcc ggtacatccg cgatcggttc
    13861 ctcagccacg ccgtgcgcga agcttatcgc gggctgatgg aggctcatcg ttacccggtc
    13921 gtgttcctgg cccttgaggt gccgcccgat tcagtcgacg tcaacgtcca tccgaccaag
    13981 atcgaggtcc gctggcgcga ttcgaacatg ctttattcgc aggtgttgtc ggccattcgc
    14041 gaccggtttc tcaacacgga tctgacgccg gggctgatgg ccgtgcggtc gccgcagatt
    14101 gccgacccga caggcgaatt cagcggggac ctgatcgaag ctcgtgaatc ggcggtcgca
    14161 gggccggaca gcgaagcgga gcggcggcat cgggagattc gcgagtcgat cgccgacttc
    14221 ttcaagcgaa acaagccgcc ggttccgtcg ggttttggtg aaggccggcc ggtttgcacg
    14281 gcctatagcc cgtcgcagcc gattgaggct tatcagccgg cgcggatggt agattccgac
    14341 gcagccttgg aatcattcga accgccgagc ctcctgccgc cggcgtccgt ccgggccggc
    14401 gagagcgaaa tcgatcgcat cgacctcggt accgtgttgc agatccatcg cagctatctg
    14461 gtgaccgaga cgtccgacgg gttggtgatc atcgaccagc acgccctgca cgagcggatt
    14521 ctttttgagc agttgtcaca ggagatcacc cgcgggccgc tcgagtcgca gcgtctgctt
    14581 cttccggaca tgatcgacgt tgcccccgac caggtggcag tgatcgaggc tcacgccgat
    14641 acgctcgcca agcttggctt cgagatgacc tcctacggcc cgagtacgat cgcggttcac
    14701 gctgtcccga gcattctcaa gcccgagcgg gtgaccgcgt tcgttcgcga catgcttgat
    14761 cgattggcgg tgcggtcggg cccggccgcg agcgagatgc tgattaatga tttgctgagc
    14821 atgatggcgt gcaaggcggc ggtcaaggcc ggcgacccgc tcgatccggc cgagatccgc
    14881 tcgctgctgg cccaacgcca ccacatcgag cgttcgagca actgccccca cggccggcca
    14941 acctcgctgc gattcacgct ccgcgatctg gaaaggcagt ttaagagggt gtagcagggg
    15001 gcttctgtta atctgttggg ctgttcggcc gctcttgacg agccgagggg cttgtcccct
    15061 cggtccgggc aggcctcctg ttattcgccg cctgtccggg aatctatcga cgaaatgcgt
    15121 atgatgtccc cggattccgc ttgaagtgga cgcggcaagg ggaagaggac atgatacggg
    15181 gcattggcat gaagcggtga ccgtggtctg cagctcgcga gcgctggatt atcttcgcaa
    15241 tacccgagca agctgtcagt ccgggtcgcg ttgaggggtg tgagacgctg gagaaggacc
    15301 tcgcacgact tgtagccagt actcagcctt cgtagccggg catgcaggag atggacgatg
    15361 ctccaaagac cttgggtgcg catcgtattc gcggggttcg ttctggcggc gttcgcgatg
    15421 ttggccgccg gggcggatgc gccggccgcc gagcggttgc ccaatatcgt gttctttctg
    15481 gccgacgaca tgggatacgg cgacccgcgt tgcctcaatc cggactcgaa gatcccgacg
    15541 ccgcacatgg atcggctcgc cgcggagggg atggtttttc gagacgctca ctcgccctcg
    15601 gcggtctgct ctcccactcg ctatggtatc ctgaccggcc gttattgctg gcggacagag
    15661 ttgaagcggg gggtgctcgt cccgtgggac cggccactga tcgagcccgg ccgtctgacc
    15721 atgccggcga tgcttcggca gcacggttat gtgaccgcat gcatcggcaa gtggcacctc
    15781 ggatgggact ggcccacccg cgacggccgg cccgttccag actcgttacg acacgcccga
    15841 tccgacggca atctcgcaat ggaagattac gaggtcatcg atttgactcg ggtcattgac
    15901 agcgggccac tgactcgcgg ttttgactcc tatttcggca tggacgtgcc caactatccg
    15961 ccgtactgct tcatcgacga cgatcggacg atcggtattc cgacgcagct caaacctgga
    16021 cggatgttcg gcgatcccgg accgatggtc cccggctggc ggctggagcc gatcctgccg
    16081 acgctgggcg cccgtgcggt cacgttcatc gatcggcacg ccgcacagca tcgtgacaaa
    16141 ccgttcttcc tctatttcgc ctcgaccgct ccgcacaccc cgatcgtgcc ggatgatccg
    16201 tttgtcggca agagccaggc cgggccctgc ggcgacctcg tttaccaggt cgattggacg
    16261 ctcgggcaga ttcttgcggc gttggaccgc aacagcatgc gcgagaacac cgtcgtggtt
    16321 atgaccagcg acaacgggtc gccggcgatc gccggtgacc cgttcaagca cggcccggat
    16381 ctcagttccc gcagcgccag tatcgccaag ttcgggcacg atcccaacgc gccatggcgg
    16441 ggcatgaagg cggacatctg ggaaggaggt catcgcgtgc cgttatttgt gcgatggccc
    16501 ggccggattg ccccaggctc gacgtgcggc cagaccgttt gcctgaccga tttcatggcc
    16561 acgtttgccg ccctggttgg gcatcgactg cccaacgatg ctgccgagga cagctatgat
    16621 ctctcaccgc tcctgctgag caaagagggc agcgaggcga tccgtgaggc cacagtccac
    16681 cactccgtcg acggcgtctt cgcgattcgc caaggcaagt ggaagctgat catgagtctg
    16741 gggtcagggg gctggtcgaa gcccaccgga ggcgagcccg aacctggtgg tccgaagggg
    16801 cagctctacg acatgcaggc cgaccccgct gaaacgaaga acctgtggac cgatcatccg
    16861 gacgttgtga aacgactgac tctgttgctg gagaagtatc agcgggacgg tcgcagcgtg
    16921 ccgcatcgga attgacggac ggttggcgcg cagattactg gagggcgcca tcgacgactc
    16981 ggacaacacg aaaggccgga gccggcagtc aggtttcgtg gtgcagctcc gtgggcagcg
    17041 ttttcagcca tccgatgctg accagacccc acaagccgat caggatggcc tcggcagcgt
    17101 catgtcgaag cgaagtgggg cgacgggcct gcgaccaggc gatgactctg cgagctacga
    17161 tgtcggcgga gtgcttggcc tgggggccgt tgcggtgctg acgtgggtag agcagcgacc
    17221 ttcgccagtc ctcggcactg attgtacgaa ggagcacgcc ccggcgctgg gcctcgtgcg
    17281 tccagatgtc ggcaagaggc cctcctccct cgagcacaag ccattcgagg tccggagtct
    17341 cacggagcac ggcctgtacg ccgcctcgca gccgcgtcgc gtttccgaag tttcgcgagc
    17401 ggtaccagcg cagccggccg tcgcgtccgt acaaggccag gccggttctc agtcccacat
    17461 cgacggccag cagcgatgac atcgtaatct gttctttccg ccgtccctgc tccgacgggt
    17521 gcagtgtgca aagtcattgg tttttggcgc gactagcctc ggcaggctgc atcaactggt
    17581 gcagggttgg aatgcccgcc gccggctggg tgctttccga ctgctccagg ctgcgggcgg
    17641 cgggcaggat gatgcggaag cgggtacctt tgccgacttc gctctggatc tccaccgtcc
    17701 cgccgtgagc cctgacaatt cgcgacacga cggccaaccc cagcccggtg ccaccgccct
    17761 tgctcttggt ggtatagaat ggctcgaaaa tgtgcggcag gtgttcggga gctattccgc
    17821 tgccggtatc gatcacatcg agcaagagtg tgcggccata gtcgcctgac cggcaacgaa
    17881 tggtcaattt gccgccattg ggcatggcat ccgcggcgtt gatcatgagg ttgatcaaaa
    17941 cctgggccat ctgcagtctg tcagccatcg caaagggcag cgattcggga agatcgcgga
    18001 tcagttcgac tcgccgccga ttcagtttcg gctgggcgaa catgagggca tcgtcgacga
    18061 tcgtttcgat gcgcgttggt tccggcgaga gcggctcgtt ccgagacatg gtcaggagac
    18121 gccgcatgat catctcgatg cggtacaggc cgttttccat gagatcgagc aactgccgga
    18181 cctggtccgg gttgtccacg cctcggcgga ggatccgaat gctgttttgc agaccatcga
    18241 gggggttatt gacttcatgg gcgacggttg cggccagttc gccgatcagt gcgagctttt
    18301 cgctgtgggt caactgacgt cgcagtcgct catgttcgcg gcagttctcc ctgagctgat
    18361 taaggactcg ctcgaccgag tcgctgagct tcaggaagtc gtcgtccggc ggatgatcga
    18421 tgcaggttgc cagatcaccg cgggcgaggg catccactgc ctctcgcagc cggtgaagtc
    18481 gtcggcgggc ctggcggc
//
LOCUS       DAOTPW010000107        18298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3077_length_18298_cov_6.149646, whole genome shotgun sequence.
ACCESSION   DAOTPW010000107 DAOTPW010000000
VERSION     DAOTPW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18298
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3077_length_18298_cov_6.149646"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..797
                     /locus_tag="P5316_11180"
     CDS             207..797
                     /locus_tag="P5316_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85561.1"
                     /translation="MPTITYGNVSWERMIRAVEKVRERLLRAAGALDRKGVPYAVADG
                     NAVAAWVSRVDEAAVRNTQDVDIVVRRADLPAATAALAQAGFVYRRVGGVDLFLDGEN
                     ARARDAVHLVFAGEKVRPEYPLPAPDVTESEQAADFRMLTLDALVRMKLTSFRDKDRT
                     HLRDLIEVGLLDRDWLDRLPPALAPRLRQLLDTPEG"
     gene            complement(911..1690)
                     /locus_tag="P5316_11185"
     CDS             complement(911..1690)
                     /locus_tag="P5316_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRR85562.1"
                     /translation="MFHQIELSARLWSAYTAQVATNPRSIGPVEGRRAYLDGCNCQEA
                     SGVIEAREFEQLPTRPIRWRYLLARPCQQSPQDRWPLLLFLHGAGERGEDLAMVKRHG
                     PLKVLEQGMDLPFVIAAPQCPPGKWWNNHMLIEFLDHLLATNQIDPDRVYLTGLSMGG
                     FGTWNLATEFPERFAAIAPVCGGGYWFLADQLRDVPVWAFHGARDDVIPLEESERMIR
                     AIQAAGGDARLTVYPEAGHDAWTQTYSNPELYQWFLSHRRT"
     gene            1815..2525
                     /locus_tag="P5316_11190"
     CDS             1815..2525
                     /locus_tag="P5316_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRR85563.1"
                     /translation="MLTRPMSELPGLSRIIEKQMRNWELGRMQRPEVFVTQRQEFAPF
                     VTIANICGAGGREVAQALSERLGWPVFDREILTLMARDDDVRESLYRSMDERDLNWFE
                     NVFRTLVQQEFRKNDYFHRLTQVVLWLARQGPAIYIGRSVDLILPQDKGLRVKIVAPM
                     ERCIDNFVRFSNVSREQARREIERIERERREFVENHFHIDPQTATRFDLQIRVDRFTT
                     SQVVDLIVCAMKARGVAH"
     gene            complement(2633..3805)
                     /locus_tag="P5316_11195"
     CDS             complement(2633..3805)
                     /locus_tag="P5316_11195"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRR85564.1"
                     /translation="MWPTLFKIPIIMVPIRSYGFMLMVGFLGGTWWAARRAARVKCDP
                     DMVVNLGFIALVASVVGSRLFYVIHYWDEHFAGRGLWAVVDITAGGLEFYGGFVGALI
                     ALLVYMKIRRVSIRLYLDIVTPSLMFGMAMARIGCFLNGCCWGGPCPEHLPWAVRFPY
                     ASPALYRQWEERQVTLPAELITINSAGIPSPLNRDLLNLTPEELEGPRVRLEQAESAL
                     NEAQTAGADEQTLTRLRKKKETRAKEKQRADNNVASLQTQLKRYHLTASEFADLARQY
                     GRTTPLHPVQLYASINGLLLAVLLNEIFYRRKRHGVVFAVLLMLYPAARIMEEMIRID
                     NPHDTAGLTISQFVSVLLFLLGVGLYYALKRMPERSPLAVPFIPPTELPPKKKRRG"
     gene            complement(3893..4741)
                     /gene="panC"
                     /locus_tag="P5316_11200"
     CDS             complement(3893..4741)
                     /gene="panC"
                     /locus_tag="P5316_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRR85565.1"
                     /translation="MKELETVSQVREYLAPHRKAGRRIGFVPTMGALHEGHLSLVRAA
                     RNDCDLVMVSIFVNPTQFGPGEDYDKYPRMLDADLVACRAAGVDAVFCPQVSEVYPAG
                     SATMVTVKRLTEGLCGAHRPGHFDGVTTVVAKLFNIVQPDVAYFGQKDAQQAIVLRRM
                     VKDLLWPIEMVVCPTVREPDGLAMSSRNAYLSPAERRQALSLSAALFRARQEILAGQK
                     DVAALVAGMRQYIESAGPCSIDYIEIVDAEELTPLTAARGRCLLALAVRIGGTRLIDN
                     VVVDAG"
     gene            4908..5519
                     /locus_tag="P5316_11205"
     CDS             4908..5519
                     /locus_tag="P5316_11205"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR85566.1"
                     /translation="MTQPDRPLFTDDTGTRDAAWVRAALSGDPDAFGRLVSAYQRVAV
                     STAYRLLGNSEDAAETAQEAFLRAYRALDRLKDPARFGPWLLRAVTNLALNARRSRRR
                     TTVALDEERGTEDAADTRGASAVTSMTPDREAEGHELQQALDAALEALPEKQRMALIL
                     FTIEGWAQKDIAELLECSVETVKWNVFQARKKLRDRLGDMIAD"
     gene            5531..6151
                     /locus_tag="P5316_11210"
     CDS             5531..6151
                     /locus_tag="P5316_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85567.1"
                     /translation="MPQISEHDEFLLSRLLDNDLSPAEAETLRRRIQDEPELGRALAA
                     MTRLDSLLKRRRADVPRVNWTTFHADVTREVARIGPYKARPQIIRLSRWLAVGLPLAA
                     AASVALVVILHRPPTKPAPGTENGGAPAFGNLSVVVHRPEPPAPAAQGKIEVNFTRSQ
                     RLAEAIWLEDAIQDEKPWWLAGGPAALPEVGPLNTDYDIAADLPPM"
     gene            6215..7450
                     /locus_tag="P5316_11215"
     CDS             6215..7450
                     /locus_tag="P5316_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85568.1"
                     /translation="MPPKTHFHCLIAHTFSPLLLLVSGLPVLEAAAPVNGPTMRVIVA
                     GTVSQPTSQPADDEAAIRVIQALDRPVDLNVADAPILEVLQSLAQSAEVSIEMETGTL
                     DLLPYGSKTLMSATIEHRSLKESLAEMLRPLGLVFEAEADRILISPTQPLRRIARRAT
                     WEELATLDKLYSTPWSSELFDLLQFQFQDARAGYADANRERLAEATDTVGPGLAAEVL
                     ELACDTRGWAWAPSGEMITIMSKTRAIERQLSRKVTVQYVQTTLEQALLDLTRRAGVS
                     LEMEPGALASLPPHVFERFPLRMEAATIRQVLDWICGLTGLSFSIEANGIRISGNKVA
                     NASNTQGDGGIGEAAIRALRANIIVGQINITNPDGSTFSFFIREDDLPPEVNEMRKAK
                     IRDAINRIRKTLESEQPKD"
     gene            7590..7943
                     /locus_tag="P5316_11220"
     CDS             7590..7943
                     /locus_tag="P5316_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85569.1"
                     /translation="MRTRTTGGVPVQLEQLVGRFDCWRRTREGRSRIPGGLWAAAVKV
                     AGRYYRTVRARDSSRHFANSARDPFADVVDPPRSVDFRCLPPADASLYPPYRPAGPRN
                     YGHAPAIRQSGGGLC"
     gene            7945..9561
                     /locus_tag="P5316_11225"
     CDS             7945..9561
                     /locus_tag="P5316_11225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR85570.1"
                     /translation="MTLVSEPTAMAKRIEIIVKTTDACNARCVYCSAGSDPGGRRELS
                     GEAAGLLLRQADELIAAGRVDRVRFLWHGGEPLLLGKDFFRELAVAFHALPHRAGITH
                     RIQTNLTLLDEEWISILEPLIGRDGLGTSLDPFDDVRQLGEAGGYRRRWLAGLELLDR
                     AGWKAGCVYVLHRPGLPRAKDLYWFFRNLRDNSMLSLRVNPLLRVGRAKETPCESLLL
                     TPGDYGRFLIEIADVWLGDHRRLILSPVWDFVRAWEGGSVSSCDLAGRAGCVEGHLGI
                     DADGFVYNCGRAVDAGGMKLGRLGEASLQECLDHPWRQVLVDREKALRRGSCGVCEYW
                     RFCHGGCPYESHAESEHAHRPTLLCGDYRLFFEWLAGKAGDQRSTVGGRPSAISGRLS
                     ATGDQRSSVSGQQEYAVVVPGAGGGIAGVRSATSCGKPVFLADLRAWLPYDLRSLAEL
                     FLRDPGLNVAIEPFYSALVRLTGGAAAGGCRSLRQMYREASPIRCPDLHCATCEAFKF
                     CRGFWLDPQPLDEHCRAWREVHAMMARLVA"
     gene            complement(9747..13115)
                     /locus_tag="P5316_11230"
     CDS             complement(9747..13115)
                     /locus_tag="P5316_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR85571.1"
                     /translation="MYCSHFGLHRPPFNNTPDPTFYFSTPEHEEALATLQYATEQRKG
                     FVLITGEVGAGKTLTGRVFLRQIEQQAETAVITNTHLSGHQLLAAICSEFGLSPPPQA
                     GNLELSQLLQEFLLEQFAQDRYVVVIIDEAQNLPDEAFEEIRMLGNLEADDAKLLQVC
                     ILGQPELRERFSQPKMRQLDQRLFGRFHLKALSAQQTRQYIHHRLRVAGCPGTDLFTD
                     EAIDAIYLASKGIPRLINQICDAALLACYADRRKVVNDEIVEEVCEQVGGVSGDAKSP
                     ETSDHPATVEAVATSPRDNRADAGATAATDVLREEATLDTLTRSIQECVETVIESRST
                     TPGMTPVDNAEIEERCRKAREELARLAGTDFSAETTDQPCPRSQETSQAGLRREGPRA
                     KKTVRSPQNGGRLADFMSAMKRKCSRIADRETGEQTEASQESTPPLKNTERTDQLDEA
                     AARKPEKEIDMLKRRLADSEQAIIDLGRHVASQFNATQEALKSLERKTAGAEEVRRLK
                     SVHEAAASDMERRLAGIAASLQQLSEWIRKRGEKGRGETLPEFEALSKRVIEQAQQLQ
                     EIRKRTLAQNASVARILAELKSQTVSRAELDEVRAAQAETGRRILERIDRNRDDTQEI
                     LDNLEERYLGLRDQIEALIASKADASSLTDIRNRHQADFGELLGALGKQRQDLENKLE
                     QVVADWKQTQKALDDLAAQAGDKRELDEIRRRQAADASRILEMLAAHRREVESLAQDL
                     DRRAGDLLARLNALPKDVATTEQLQDIRAAYTEQITDLTARLAACEAGFEKTRELHER
                     GLRAVAGKTLDTARRVSILEEKGRPRRIRLELSPRAGIELGDVVAAAQEQRDSLRADI
                     EEAGKTAADLREMSSTIREATQAWQASLEASWDNAQAFLQEWQKKAVEAKGEADRMLD
                     GWRRGTDEVRQEAERLRASARMAAEVLQRMRQCHAAIDAKLNSKEWQAVFSRAESTAS
                     RLEQAVAAGQTVCQQIAGRIEQAIAAGQTISQQIAGKTEQSKNELAAYLADCKKQLAK
                     RLAEFAAEQQQMDRVIEARRQMLARIAKSAGSLVEVIDAGRQFDEQRHPSVPADPASK
                     PPAVESRDLQADTVAEIRWPRFRTHQPQTQAQAG"
     gene            13395..14222
                     /locus_tag="P5316_11235"
     CDS             13395..14222
                     /locus_tag="P5316_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRR85572.1"
                     /translation="MQDFAKTRLMILGMGALVQLVASTGCAPKYDDVKAFVQAHNHDV
                     VDATHRAKPTDLVVIASATCPEVDGVNSRINPDGTIMLRLLGPVKVSMLTEQEIAAKM
                     EKLLARYYVDPKVEVRLVTPTSRCIYVFGQVTFNGPKPFTGNDTLLTVLANARPNLIA
                     WGEMVRVIRPSADPNEVREITIDVTRMIQKGDLRNNLLLQEGDIVYVPPTPLGWVGLR
                     VQELLYPAVPLLNAYAFPANANNTLRTYTDYNWSDGTGANTELYYGYGVRPYINEGW"
     gene            14266..16029
                     /locus_tag="P5316_11240"
     CDS             14266..16029
                     /locus_tag="P5316_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85573.1"
                     /translation="MSVQPTSVILGPRIGAPPEESAVASLLQTLSKVLDVARRHWPLI
                     TISMLTVMLLVLSIGLTCPRRYYVSTTFERRNNLVISKLLASNSPYSFAALRQSLISD
                     LRSFSIVGLAVEEAGLLDHLPRDGSGNLTAEAKADRDAIIGRWIESITVHIRETGDFS
                     DIVEILYLGDQPDRAAPILARIRDSYITGTRNRVSGILNDAHEFFERESRKCQEKVAA
                     LRAELHRIDVDFPGTNPTGPDTLEQRLQVVARSIERNERDRQRLQARIASSEAYLAGL
                     TTRPSQDAAQPNRPAAIGTSTMANPRYQQTTAEIASLNRKIDDLKVTRRMTEMHPQIV
                     TLRRQVESLQETLNQLPENLEVPSGMMNLPADSWEAERKKTEMEIKTNRDDLARIELE
                     LKANLETQERLKKDMQSLPERRERYVNLQQALASAQNDLDLWNNNLSQLNRILTAEAE
                     NRGIQVLPLDQPHLAGRLRRPTANGLFAVSSGLGLAVAIVIVFLREILDRSVKDPARL
                     KQTLGVPVLETIGEIRAGRPSRWRLRKCLLPTFVTVQTLAVALMGILVYLALERPESY
                     DRLMTRSAALLGEWTGRITGT"
     gene            16087..16995
                     /locus_tag="P5316_11245"
     CDS             16087..16995
                     /locus_tag="P5316_11245"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HRR85574.1"
                     /translation="MGRIAEALKRAQQERNLRLAQTTDMSFGNVAAASVRTFGRGATR
                     AESFSDCVQSLLKPPSSPQPFPITAPAVESESVGGEVVALHEPNSVTAEKFRSVRTRL
                     ITANPGGGPRVLALTSALHGEGKTVTTANLAFSFAELKYLRVAMVDLDFHACGLSCLL
                     GAERQPGLAEVLRGELSLAEVCMPAVRDNLYLIPAGDPAGLLPSELLASKSFGGVFKE
                     LTERFHYSLIDTPPVHEAADTGVIAPLCHSVIMVVRMNRTPEPLVDRCVKMLQANRVN
                     VAGCVLAGYHETRMASLSHDYYQSTS"
     gene            17005..18069
                     /locus_tag="P5316_11250"
     CDS             17005..18069
                     /locus_tag="P5316_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HRR85575.1"
                     /translation="MSISITELDEGTTGLWDAYVAGHPESTIFHTLIWRDAVTEAFGH
                     RSHYLMAFRDGRPAGVFPLMRVDSRLAGTLMVSVPYAVYGGSLADDDETHRALLEAAT
                     ALAIQAGAQWLDIRSIRARWPELPISDRYVTFRKSLPADPNRVLAEMPRKARAAARQA
                     RERFNLVSRFDESRLDEVWLLYSQSMRRLASPNYPLRFFRALMDATRSRLQAGERVEH
                     LVQIVEHQGRAIAGLVSFVYRDTLSPYFAGCDERFEKYHPNHFMYMCAMEKGVERGCR
                     EFDFGRSRVDNKGSCDFKRFQGFEPTPLQYQYFVPAGGRAPDLTPNNPRLQLSRRVWP
                     KLPLAVTRPLGAWLSKSIPG"
BASE COUNT         3287 a         5400 c         5930 g         3681 t
ORIGIN      
        1 ctgtgcagga gatcttccag gccgccgccc ggtggacctt catcggcaca catcgcgaca
       61 agaccgatcc gcatgaacgg gtggtggagt tgcagttgcg gtaggctaag gaccgctgga
      121 ccggccgggg atcagaaact gccttcttgt ttcgctatgc cggtggttag aatatcggga
      181 gagatttgct atgggcctga gaacccatgc caacgatcac gtacggtaac gtctcttggg
      241 aaaggatgat ccgagccgtg gagaaagtgc gcgaacgatt gctgcgggct gcgggcgccc
      301 tcgacaggaa aggggtgccc tatgccgtgg cagacggcaa cgcggtggcc gcctgggtct
      361 cacgagtgga tgaagccgcc gttcgcaaca cgcaggatgt cgatatcgtg gttcgcaggg
      421 cagacctgcc ggccgctacg gcggctctgg cccaggccgg attcgtctac cgccgcgttg
      481 gcggcgtcga tctgttcctg gatggcgaga atgccagagc gagggacgcc gtccacctgg
      541 tgttcgccgg ggagaaagta cggcccgaat acccattgcc cgcccctgac gtgaccgagt
      601 cggagcaggc cgcagacttc cgcatgctca cgctcgacgc actcgtgcga atgaaactca
      661 cctccttccg cgacaaagac cggactcacc tgcgggattt gatcgaggtg ggactgctgg
      721 accgcgattg gctggatcgc ctgccgccgg cactggctcc tcgtttgagg cagttgctcg
      781 acaccccaga gggatagagg gcatctggct gggcgtcgtc gccgccttga tattcatcac
      841 agctctgctt gtgcccacgt tgagttgaga ccaaacgtcg ggttcatgcg acacacctct
      901 atttcccatc tcatgttcgc cggtggctga ggaaccactg gtagagttcg gggttggagt
      961 acgtttgcgt ccatgcatcg tgcccggcct ccgggtaaac cgtaagccgg gcatcgcccc
     1021 cggcggcctg aatcgcccga atcattcgct ccgattcttc cagcgggatc acgtcatccc
     1081 tggccccgtg aaaggcccat accggaacgt ccctgagctg gtccgcgagg aaccagtatc
     1141 ccccaccaca gaccggagcg atggccgcaa agcgttccgg aaactcggtg gccaggttcc
     1201 aggtcccgaa accgcccatg ctcagccccg tcaggtacac gcgatctgga tcgatctggt
     1261 tcgtcgccag caggtggtcg aggaactcga tgagcatgtg gttattccac cacttgccgg
     1321 gcgggcactg gggggcggcg atgacgaacg gtagatccat gccctgctcc agaaccttga
     1381 gcggtccgtg gcgcttcacc atggccaggt cttcgcctcg ttcgccggcc ccgtgaagaa
     1441 acagcaatag cggccagcga tcctggggcg actgctgaca cggccgggcc agtagatatc
     1501 gccaccgaat ggggcgggtc ggaagctgct cgaattccct ggcctcgatc acgccggagg
     1561 cctcctggca gttgcaaccg tccagatacg cgcggcggcc ttccacaggt ccgatactcc
     1621 gcggattggt cgcgacctgc gcagtatacg cggaccacag gcgagcggac agctcgatct
     1681 gatgaaacac tgggaccttg cctgccaggg gtcttgcatc ctgtgcggca acgacgtacc
     1741 ttgaagagag accatctcgt ccgttgtctg acgtccggga cacgaccggg cgaatgtcca
     1801 gaagaggtgc aaccatgctg accagaccga tgtcagaact gccgggcctc tcaaggatta
     1861 tcgagaaaca gatgcggaac tgggagcttg ggcggatgca acgtccggag gtgtttgtca
     1921 cccagcgtca ggaatttgcc cccttcgtca ccatcgccaa catatgcggc gccggcggca
     1981 gagaggttgc tcaggcgctg tccgagcggc ttggctggcc ggtattcgac cgcgagattc
     2041 tcaccctcat ggctcgcgac gacgatgttc gtgaatctct ctaccgttcg atggacgagc
     2101 gcgacctgaa ctggttcgaa aacgtgtttc ggacgctggt ccagcaggaa ttccgcaaga
     2161 acgactactt tcatcgtctg acgcaggtgg tcttgtggct ggcgcggcag ggtccggcca
     2221 tctacatcgg tcggtcggtc gacctgattc tgcctcagga caagggcttg cgggtcaaga
     2281 tcgtggcccc gatggaacgg tgcatcgaca acttcgtccg gttcagcaac gtcagtcgcg
     2341 aacaggctcg gcgggagatc gaacgcatcg agcgggaacg acgggaattc gttgaaaacc
     2401 acttccacat cgacccgcag accgcgacgc ggtttgatct gcaaatccgc gtggaccggt
     2461 tcaccacgtc gcaggtggtg gacctgatcg tctgcgcgat gaaggcgcga ggcgttgctc
     2521 actgagataa ccggctcact taaggcgaac cggctttttg cgagccggaa ccgaagcaca
     2581 ctacgttcca gaaacacgcg cccaatctgc acacacgtct ggccgtaagg ttctaacccc
     2641 gacgcttctt cttggggggg agctccgtgg gaggaatgaa ggggacggcc aaaggcgaac
     2701 gttcaggcat tcttttaaga gcgtaataca ggccgacgcc gagcagaaac agcaggacgc
     2761 tgacgaactg ggagatggtc aggccggcgg tatcgtgcgg attgtcaatg cggatcatct
     2821 cttccatgat ccgggcggca ggatagagca tcaggagaac ggcaaaaacg acgccgtggc
     2881 gtttgcggcg atagaagatc tcattcagca aaacggccag gagcagccca ttgatggagg
     2941 cataaagctg cacggggtgg agaggcgtcg tcctgccgta ctgtcgggcc aagtcggcga
     3001 attccgatgc cgtcaggtga tatcttttca actgggtttg cagacttgcc acgttgttgt
     3061 ccgctcgttg tttttccttc gcccgcgttt cttttttctt gcgcagcctg gtcagggtct
     3121 gctcatcggc tcccgcagtc tgggcctcgt taagagccga ctcggcctgt tcaagcctaa
     3181 cgcgcggccc ctccaactcc tcaggggtca aattgagcag atcacggttg agtggtgagg
     3241 gaataccggc ggaattgatg gtgatcagtt cggcgggcaa tgtcacttgg cgttcctccc
     3301 actgcctgta cagtgccgga ctggcatatg ggaacctcac cgcccacggc aggtgctcgg
     3361 ggcaggggcc gccccaacag cacccgttca ggaaacaccc gatcctggcc atggccatgc
     3421 cgaacatcaa agacggcgtc acaatgtcga ggtacagacg gatggatact ctgcggatct
     3481 tcatgtagac gagaagagca atcagggctc cgacgaagcc gccatagaac tcgaggccgc
     3541 cggcggtgat gtccacgacg gcccacaaac cccgacccgc gaagtgctcg tcccagtagt
     3601 gaatcacata gaacagtcgc gacccgacca cgctggccac caaagcgatg aatccgaggt
     3661 tgaccaccat atcggggtcg cacttgaccc ttgccgcccg ccttgccgcc caccaggtac
     3721 cgccaaggaa cccgaccatg agcatgaaac catagctgcg aatcggcacc atgatgatgg
     3781 ggatcttgaa gagcgtaggc cacatattca ggggttctcg ctgttgattt catggcggcc
     3841 agtggcgtgc agcgccgctt ggccgtcgga tcaaggcatc ttaacggtcc gctcagccag
     3901 cgtccacaac gacattgtcg atcaaccgcg tcccgccgat ccgcacggcc agagccaaca
     3961 ggcatctccc gcgcgcggcg gtcagcggag tcaactcttc tgcgtcgaca atctctatgt
     4021 aatcaatgct gcacgggccg gcggactcga tgtattgccg catgccggcg acgagagcgg
     4081 ccacatcctt ctgtcccgcc agaatctcct ggcgggcccg gaaaagggct gccgagaggc
     4141 tcaaagcctg tcgacgctca gcagggctga gataggcgtt tcggctcgac atcgccagac
     4201 cgtcgggctc tcgaacagtc gggcacacga ccatctcaat aggccagaga aggtctttga
     4261 ccatccgccg cagaacaatg gcctgctggg cgtccttctg tccaaaatac gccacgtcgg
     4321 gctggacgat attgaataac ttggccacga cggtggtcac gccatcgaaa tgcccagggc
     4381 gatgcgcacc acataggccc tcggttagac gcttaaccgt caccatggtt gcggaaccgg
     4441 ccggatagac ctctgatacc tgcgggcaga agacggcgtc gacgccagcg gcccggcagg
     4501 cgaccaagtc cgcgtcgagc atacgaggat acttgtcata gtcctctccg gggccaaact
     4561 gggtcggatt cacgaagatc gagaccatta ccagatcgca atcgttacgg gcagcccgca
     4621 ccaaggacag atggccctcg tggagagccc ccatcgttgg cacaaagccg atccgcctgc
     4681 cggccttgcg atggggagca aggtattcgc ggacctgcga gactgtctct aactctttca
     4741 ttgacaagca cctaaagcct tttcaccgat cggtgtcgca tccacccgct tcagcccgat
     4801 attatggact tacgggcgtt gacagggtag taccaagccc gatagactcg ttcacccgcg
     4861 ttaattctcc cgcatccaac cgttttcgag caacggtgtc tatatctatg acgcaaccag
     4921 accggccgct cttcacggac gacaccggga cgcgtgatgc tgcgtgggtg cgggcggcgc
     4981 tgtctgggga ccctgacgct ttcggacggc tggtgtctgc gtaccaacgg gtggcggtct
     5041 cgaccgcgta tcggttgctg ggcaacagtg aagatgcggc tgagacggcc caggaggctt
     5101 ttttacgagc ctatcgcgca ctagatcgat tgaaagatcc ggcccgcttt gggccgtggc
     5161 tgctgcgtgc ggtgaccaac ctggccctga acgcgcgtcg atcgcggcgg cggaccacgg
     5221 tggcgctgga cgaggaacgg ggtaccgagg atgcggcgga cacccgcggc gcgtcggcgg
     5281 ttacctcgat gacgcccgat cgcgaggccg agggtcacga gttgcaacag gcgctcgatg
     5341 ccgccttgga agcgttgccc gaaaagcagc gcatggccct gatcctgttt accatcgagg
     5401 gctgggcgca aaaggatatc gccgaattgt tggaatgcag cgtggaaacg gtgaaatgga
     5461 acgtattcca ggcgcgtaag aagttgcggg accgactggg ggacatgatt gccgactgag
     5521 gagcatgata atgccgcaga tcagcgaaca tgatgaattc ctgctgagtc ggctgttgga
     5581 caatgatctg tctccggcgg aagcggagac tctgcgccgg cggattcagg acgagccgga
     5641 gctgggtcgg gccctggcgg ctatgactcg cctggacagc ctgctgaagc ggcgccgtgc
     5701 cgatgtgcct cgagtcaact ggacaacgtt tcacgccgac gtaacccggg aggtggcaag
     5761 gatcggcccg tacaaagccc ggccgcagat catccggctt tcccgctggc tggccgtcgg
     5821 gctgcctcta gcggccgccg catccgttgc tctggtggtg attctgcata ggccgccgac
     5881 caagcccgcg ccgggtaccg agaacggcgg tgcgccggca ttcggaaatc tgtcggtggt
     5941 ggttcaccgg ccggaaccgc ccgctccggc agctcagggg aaaatcgagg tcaacttcac
     6001 ccgttcgcag cgcctggccg aagcgatttg gctggaagac gccatccaag acgagaagcc
     6061 ctggtggctg gccggtggtc ctgcggcctt gcctgaagtc ggtccactga atacagatta
     6121 cgacattgca gccgatttgc cccctatgta acggctacgg ctgctactta cccactggcc
     6181 gaggttaaac ccgttttgct gacaggaaga cacgatgccg ccaaagacac atttccactg
     6241 cctcattgcg cataccttct cgcccctcct gcttcttgtt tcaggtcttc cggttctgga
     6301 agccgccgcg ccggtcaacg gcccgaccat gagggttatt gtcgccggga ccgtctcgca
     6361 gcccaccagc cagccggccg acgatgaggc ggccatccgg gtcattcaag cattggatcg
     6421 gccggtagac ctgaacgtgg ccgatgcgcc gatcttggag gtcctgcagt ctctggctca
     6481 atccgccgaa gtctcgatcg agatggaaac ggggaccctg gatctactcc cttacggttc
     6541 caagacgttg atgtcggcga cgatcgagca tcgctcgctg aaggaatcgc tagccgagat
     6601 gcttcgcccg ctggggctgg tctttgaggc ggaggctgac aggatcttga tatcgccgac
     6661 ccaaccgctt cggcggattg cccgccgggc aacgtgggag gaactggcca cgctggacaa
     6721 gctttactca acaccctgga gcagtgagct tttcgacttg ctgcagtttc agttccagga
     6781 tgctcgagcg ggctacgcgg atgcaaatcg cgaacggttg gccgaggcaa ccgacacggt
     6841 ggggccgggg ctggcggccg aggtgctcga actggcctgc gacactcgcg gctgggcttg
     6901 ggcgccatcc ggggaaatga tcaccattat gagcaagacc cgggcgatcg agcgccaact
     6961 cagcaggaag gtaacggtgc agtacgttca gaccacgctg gagcaggctc tgctggatct
     7021 gacccgccgg gccggcgtgt cgttggagat ggagcccggg gctcttgcct cgctgccgcc
     7081 gcatgtgttc gagcggttcc cgttgcgcat ggaggctgct acgatccggc aggttctcga
     7141 ctggatatgc gggttgaccg ggctcagctt ctccattgag gcaaacggca tccgcatatc
     7201 cggcaacaag gttgcaaacg cgtccaacac ccagggtgac ggcgggatcg gcgaggctgc
     7261 catccgggcg ctgcgagcca acattatcgt cggccagatc aacatcacca accccgacgg
     7321 ctcgacgttc tctttcttca tccgcgagga tgacctgccg ccggaagtga acgagatgcg
     7381 caaggccaag atccgcgacg ccatcaaccg gattcgcaag acactggaaa gcgagcagcc
     7441 gaaggactga gtcgtgtccc cgcggcatcc gccatcctcg accggtgcct ccagcacgcc
     7501 gagggtgtcc ttcctgtaag cctgcatgga tcgcgatgaa tcgtccggta tcgtccgctg
     7561 tcagtgcgag attgcacgga gaacagccga tgagaaccag aacgacgggc ggcgttcctg
     7621 tccagttgga gcaacttgtg gggcgtttcg actgctggcg gcggactcgc gagggccggt
     7681 cccggattcc tgggggcttg tgggccgcgg cagtgaaggt cgccggcagg tattaccgca
     7741 ccgtccgggc ccgagactcc tcccgccatt tcgccaactc tgctcgtgat ccgttcgccg
     7801 acgtcgtcga tcctccacgg tccgttgatt tccgttgcct gcctcctgcg gacgcctcgc
     7861 tctatccccc atatcggccc gccggcccac gcaactacgg ccatgcaccc gcaatcaggc
     7921 aatcaggagg cgggttgtgt tagaatgacc ttggtttcag agccgacagc gatggccaag
     7981 cggatcgaga tcatcgtcaa gaccaccgat gcatgcaacg cgcggtgcgt gtactgttcg
     8041 gccgggagcg atccaggcgg ccgtcgggag ctgtcgggcg aggcggcggg tttgctgctg
     8101 cggcaggctg acgagttgat tgcggccggc cgtgttgatc gcgttcgatt tctctggcac
     8161 ggcggcgagc cgctgctgct gggcaaagat ttctttcgcg aacttgcggt cgcgtttcac
     8221 gcgttgcccc accgtgcggg gatcacgcac cggattcaga ccaatctcac gttactggat
     8281 gaagagtgga tctcgattct ggagccgctg attggccgtg acgggcttgg aacgtcgctc
     8341 gatccgtttg acgacgtgag gcaattgggc gaagcgggcg gctaccggcg gcgatggctg
     8401 gccggcctgg agctgctcga tcgtgccggc tggaaggccg gctgcgtgta cgtgctgcat
     8461 cggccgggcc tgccgcgggc caaggacctc tactggttct tccggaacct gcgagacaac
     8521 tcgatgctgt cgctgcgcgt caatccgctg ctgcgggttg gccgggcgaa ggagactcct
     8581 tgcgagtcgc tgttgctgac gcccggcgat tacgggcggt ttctgatcga gatcgccgat
     8641 gtctggctgg gcgatcatcg tcgcctgatt ctctcgccgg tgtgggattt cgttcgggcc
     8701 tgggagggcg ggtcggtaag ttcctgtgat ctggccggcc gtgcggggtg cgtcgaaggg
     8761 catttgggca tcgatgccga cgggtttgtc tacaactgcg gacgggcggt tgatgcgggc
     8821 gggatgaagc tcgggcggct cggcgaggcg tcgctgcagg agtgcctgga tcatccttgg
     8881 cggcaggtgc ttgtcgaccg cgagaaggcg ctgcgacggg ggtcgtgcgg ggtctgcgag
     8941 tattggcgat tctgccacgg cggctgcccg tatgagtcgc acgccgagtc ggagcacgcc
     9001 catcggccga cgctgttgtg tggggattac cgtttgtttt ttgaatggtt ggcagggaag
     9061 gcgggcgatc agcggtcaac agtcggcggc cggccatcgg ctatcagcgg tcggctttca
     9121 gcgaccggcg atcagcgatc atccgtgagc ggccagcagg aatacgcggt agtggttcca
     9181 ggtgccggcg gcggcatcgc gggtgttcgt tcggcgacgt cttgtggcaa gcctgtcttc
     9241 ctcgccgatc ttcgtgcctg gttgccctac gatctgcggt cgctggcgga gttgttcctg
     9301 cgcgatcccg gtctgaacgt ggccatcgag ccgttttact cggcactcgt gcgtctgacc
     9361 ggcggtgctg cggcgggtgg ttgccggtcg cttcggcaga tgtaccgcga agcctcgccg
     9421 atccgctgtc cggatttgca ttgcgccacc tgtgaagcct tcaagttctg tcggggtttc
     9481 tggcttgatc cgcaaccgct ggacgagcat tgccgcgcgt ggcgggaggt gcacgcgatg
     9541 atggcgaggc tggtggcctg atgagggcgg cagctcggcg cttcgctttg acctggccta
     9601 cggggttgat tcgtctcggc tttcggcagc cgggcaacga agggtcgggt gcagatacaa
     9661 gcggcaggtc ggcgcttcgc tttgacctgc cctactgtgc cgaagcgggt atgtcttgag
     9721 cgcggcggat cggcggaagg tcgtgttcag ccggcctgag cctgagtctg cggctgatgg
     9781 gtgcggaacc ggggccagcg gatctcggcg accgtgtcgg cctggaggtc gcgcgactcg
     9841 acggccggcg gcttggaggc agggtcagcc gggacagagg gatgtcgttg ttcatcgaac
     9901 tgccgtccgg catcgatcac ttcgaccagg ctgccggcgc tcttggcgat tctggccagc
     9961 atttgtcggc gagcctcgat gacgcggtcc atctgttgtt gctcggcggc gaattcggca
    10021 agtcgctttg ccaactgttt cttgcagtcg gccaagtagg cggcaagttc attcttcgat
    10081 tgttcggttt tgccggcgat ctgctgggag atggtttgac cggccgcaat ggcctgctcg
    10141 attctgccgg cgatttgctg acagacggtc tggccggccg caacggcctg ttcgagacga
    10201 ctcgcggtgc tctcggctct ggagaacacc gcttgccatt ctttcgaatt aagtttggcg
    10261 tcaatcgccg cgtggcactg tcgcattctc tgcagaacct ccgcggccat gcgggccgaa
    10321 gccctgagtc gctcggcctc ttggcggact tcatcagttc ctctgcgcca gccgtccagc
    10381 atccggtcgg cctcgccctt tgcttcgacg gctttcttct gccattcctg cagaaatgcc
    10441 tgcgcgttgt cccagcttgc ttcgaggctt gcctgccaag cctgcgtcgc ctcgcggatg
    10501 gtggaggaca tttctcgaag atcggcggcc gtcttgccgg cttcctcgat atccgcgcga
    10561 agcgaatcgc gctgctcctg tgccgcggct acgacgtcgc ccagctcaat gccggcgcgt
    10621 ggcgacagtt cgaggcggat tctgcgcggc ctgccttttt cctcgaggat tgacacgcgg
    10681 cgagccgtgt caagggtctt tcccgccacg gctcgcaagc cccgttcgtg gagctcgcgc
    10741 gttttctcga aacccgcttc gcaggcggcc aagcgagcgg tcaggtcggt gatctgctcg
    10801 gtgtaggccg cacggatgtc ctgaagctgt tcagtggtgg cgacatcctt gggcagggca
    10861 ttgagtcgcg cgagcagatc gccggcgcgg cgatcgagat cttgcgcaag ggattcgact
    10921 tcccggcggt gagcggcgag catctcaagg attcggctcg catcggcggc ctgtcgacgc
    10981 cggatctcgt ccagttcccg cttgtcaccg gcctgggcgg ccagatcgtc cagggccttc
    11041 tgggtctgtt tccaatcggc tactacttgc tcaagcttgt tctccaggtc ctgtcgctgc
    11101 tttccgaggg cgccgagcag ctcgccgaag tcggcctgat gtcgattccg gatgtcggtg
    11161 aggctcgagg cgtcggcttt ggacgcgatc agtgcctcga tctgatcccg cagcccgagg
    11221 tatcgctctt cgaggttgtc gagtatctcc tgggtgtcgt cgcggttgcg atcgattcgt
    11281 tcaaggattc ttcgccccgt ttcggcctgg gcggcgcgca cctcatccag ctcagcacgg
    11341 ctgaccgttt gacttttcag ttccgccagg atccgggcga ccgacgcatt ctgggccagg
    11401 gtgcgctttc gaatctcctg gagctgctgc gcctgctcga tgacccgctt ggacagcgcc
    11461 tcaaactcgg gcagcgtttc acctcggcct ttttcgcccc gcttcctgat ccattcactg
    11521 agctgctgga gcgaggccgc gatgccggcc agccgacgct ccatgtccga cgcggcggct
    11581 tcgtgaactg acttgagccg tctgacctcc tcggcgccgg cggtcttcct ttccagagac
    11641 ttcagtgctt cttgcgtggc gttgaactgt gaggcaacat gccggcccag gtcgataatt
    11701 gcctgctcgc tgtccgcgag tcggcgcttg agcatgtcga tttccttttc gggtttccgg
    11761 gcggctgctt catccaactg atcagtcctc tcggtgtttt tcaatggcgg cgtcgattcc
    11821 tgagatgctt cggtttgttc gcctgtctcg cggtcggcga ttcgcgagca tttccgcttc
    11881 atggcgctca tgaagtcggc cagccgtcca ccgttttgcg gggaccggac ggttttcttg
    11941 gctctcggtc cttcgcggcg cagtccggct tgcgacgtct cttggcttcg tggacatggt
    12001 tgatcggttg tctcagcgga gaaatccgtt ccggccaggc gcgccagttc ttctctagcc
    12061 tttcggcagc gttcctcgat ctcagcattg tcaacggggg tcatgccggg agtcgtgctg
    12121 cggctttcga tcaccgtttc gacgcattcc tggatcgagc gggtcaaggt atcgagggtt
    12181 gcttcttccc gcaggacgtc tgttgcggcc gtggccccgg catcagccct gttgtctcgg
    12241 ggggaggttg caaccgcctc gacggttgcc ggatgatctg acgtctcggg ggatttggcg
    12301 tctccactga cgccaccgac ctgttcgcag acttcctcaa cgatctcatc gttgacgact
    12361 ttccgtctgt cggcgtaaca ggcgagcagc gcggcatcgc agatctggtt gatcagtcgg
    12421 ggaattccct tggacgccaa gtagatggca tcaatggctt catcggtgaa aaggtcggtt
    12481 cccgggcatc cggcgacgcg aaggcggtga tggatgtact ggcgcgtttg ttgagcggat
    12541 agggccttga ggtgaaaacg accaaacagc ctctgatcca actggcgcat tttcggttgg
    12601 gagaatcgct ctctcagttc aggttgtccc aggatgcaga cttgcaggag cttggcgtcg
    12661 tccgcctcaa ggttgccgag catccggatc tcttcgaagg cctcgtccgg cagattctgg
    12721 gcctcgtcga tgatcacgac gacatagcgg tcttgggcga attgctcgag gaggaactcc
    12781 tggagcaact ggctcaattc gaggtttccg gcttgcggag gcgggctgag cccgaactct
    12841 gagcagattg cggccaggag ctggtggccc gacaggtggg tgttggtgat cacagcggtt
    12901 tcggcctgct gttcgatctg gcgcaggaac acccggccgg tgagcgtctt gccggcgccg
    12961 acctcgccgg tgatgaggac gaatcctttg cgttgttctg ttgcgtactg aagggtggcc
    13021 agggcctcct cgtgttccgg cgtactgaag tagaacgtcg gatccggggt gttgttgaac
    13081 ggcgggcggt gaagcccgaa atgggagcag tacatgcccg gtacctcctg atggggagac
    13141 cgttttcggc gcgcgcgatt atcggacgaa ggagaaccgg cgcgtcccaa gccgttctat
    13201 cggacctgcc tggtgggcac cttgaggctc gtgccaacgc ccggaaaaaa aacgaggata
    13261 agtcgaactg agcctctgag cgtccgatta ctctggcacg gagccaggtc cgccagctat
    13321 cgggcgtgcc ggctgaagcc gtcgtcgagg cagaaccgga gaggttcagg attgccacct
    13381 gtgagaccaa aggcatgcag gacttcgcca agacccgatt gatgattctg ggaatggggg
    13441 cccttgttca gcttgtcgcg agcacggggt gcgcgccgaa atatgacgac gtgaaggctt
    13501 tcgtgcaggc ccataaccat gacgttgtgg acgccactca tcgagccaag cccaccgatc
    13561 tggtggtgat tgcctccgcc acgtgtcccg aggtcgacgg ggtcaacagt cgcatcaatc
    13621 ctgacggcac gatcatgctg cggttgctgg ggccggtgaa agtctcgatg ctgacggagc
    13681 aagagattgc cgccaagatg gagaagctgc tggcccgcta ttacgtcgat cccaaggtgg
    13741 aggtccggct ggtcacgccg accagccggt gcatctacgt gtttggtcag gtgaccttca
    13801 acggtcccaa gcccttcacc ggaaacgaca cgctgttaac ggtgctggcg aacgcgcgac
    13861 cgaatctcat tgcctggggc gagatggttc gggtcatcag gcccagcgcc gaccccaatg
    13921 aggtgcggga gatcacgatc gacgtaacgc gaatgattca gaaaggtgat cttcgcaata
    13981 atctgcttct tcaggaaggc gacatcgtct atgtgccgcc gacgccgctg ggctgggtcg
    14041 gtctgcgggt gcaggagttg ttgtatccgg cggtgccgct gttgaatgcc tatgcttttc
    14101 cggcgaatgc gaacaacacg ctgcggacgt acacggacta caactggagc gatggcacgg
    14161 gggcgaacac ggagctgtat tacgggtacg gggtgaggcc gtacatcaac gagggctggt
    14221 gaggatcatc cgtcaaggca gacaagggcc cgcaaaggat ctgatatgag cgtgcaaccc
    14281 acgtcggtta ttctcggtcc ccgaatcggc gcaccgcccg aggaatcggc tgttgcgagt
    14341 ctgctccaga ctctgtcgaa ggtactggac gtcgcgcgcc ggcactggcc actgatcacg
    14401 atctcgatgt tgacggtcat gttgctcgtg ttatcgatag gcctgacctg tccccggcgt
    14461 tactatgtca gcaccacgtt tgaacgtcgc aacaacctgg tcatctccaa gctgttggcc
    14521 agcaactcgc cgtactcttt tgccgccctg cggcagtctc tgatttccga cctgcggagc
    14581 ttctcgatcg ttgggctggc ggtcgaggaa gcgggtcttc ttgatcactt gccgcgcgac
    14641 ggctccggca acctgaccgc cgaggccaaa gccgatcgcg acgcgatcat cgggcgatgg
    14701 atcgagtcga ttacggtgca catccgcgaa accggggatt tctccgacat tgtcgaaata
    14761 ctctatctgg gcgatcagcc cgaccgggct gcgccgatcc tggcgaggat ccgggacagc
    14821 tacattacgg gcacgcgcaa cagggtttcg ggcatcctca atgacgcgca cgagtttttc
    14881 gagagagaat ccaggaagtg tcaggagaaa gttgccgccc tgagggccga gcttcaccgg
    14941 atcgacgtgg actttccggg gaccaacccg accgggcccg acactctcga gcagaggctt
    15001 caggttgtcg cgcggagcat cgagcgcaac gaacgcgatc ggcagagact gcaggcaaga
    15061 atcgcctctt ccgaggccta tctggctggg ttgaccaccc gcccctcaca ggacgccgca
    15121 cagcccaatc gaccggccgc gatcgggacg agtaccatgg cgaacccgcg ctaccagcag
    15181 acgacggcag agatcgccag cctgaacagg aagatcgacg acttgaaagt cacccgtcgg
    15241 atgaccgaga tgcatcctca gatcgtgacc ctgcgtcgtc aggttgaaag cctccaggaa
    15301 actctcaacc agcttccgga gaatctggag gtgccgtcgg ggatgatgaa tctcccggcg
    15361 gattcatggg aggcggagcg aaagaagacg gaaatggaga tcaagaccaa ccgcgacgat
    15421 ctcgcccgga tcgagctgga gctgaaggcc aatctggaga cgcaggagcg actcaagaaa
    15481 gacatgcagt cgttgcctga acgcagggag cggtacgtca atctgcagca ggccctggcc
    15541 agcgcccaga acgatctgga tctctggaac aacaacttga gccagttgaa tcgcatcctg
    15601 accgccgagg cggaaaaccg aggcatccag gtgctgcctc tggatcagcc gcacctggcc
    15661 ggtcgtcttc gcagaccgac cgccaacggc ttgtttgcgg tcagttcggg gttgggattg
    15721 gccgtggcga ttgtgatcgt attccttcgc gagattctgg accgttcggt gaaggatccg
    15781 gcccggctaa agcagacgct gggcgttccg gtattggaga cgatcgggga gattcgtgcg
    15841 gggcgacctt cgcggtggcg tcttcgcaag tgtctgctac cgacgttcgt gacggtgcag
    15901 acactggcgg tagccctcat ggggatcctg gtttacctgg cgttggaacg tcccgaatcg
    15961 tatgaccggc tgatgacgcg gtccgcggct ctgttgggag aatggacagg ccgaattact
    16021 ggaacatgag agacgcctgt ccggaccgga ggatcagacg gcgtcaatag caggcgacgg
    16081 aagaacatgg gacggattgc tgaagcgctg aaacgagcac agcaggagcg gaacctgcgg
    16141 ctggcgcaaa cgacggatat gtcgtttggc aatgttgccg ccgcttccgt gcgcactttc
    16201 gggcgcgggg ccacgagggc ggagtccttt tccgattgtg tccagtcgct gctgaagcct
    16261 ccttcgtcgc cacaaccgtt tccgatcacg gcccctgccg ttgaaagtga gtcggtcggc
    16321 ggggaggtgg tggctctaca cgagccgaac tcggtgacgg ccgagaagtt tcgatccgtc
    16381 cgcacccggt tgatcaccgc caatccaggg ggcggtcccc gtgtgctggc cctgacctcg
    16441 gccctgcacg gcgagggcaa gaccgttacc accgccaatc tggcattcag ttttgcggaa
    16501 ctgaagtacc tgcgggtagc catggtggat cttgattttc acgcctgcgg gctgagttgt
    16561 ctgctcgggg ccgagcgtca gccggggctg gcggaggtct tgcgaggcga gctgtcgctg
    16621 gcggaggtat gcatgccggc ggtccgggat aatctctacc tcatcccggc cggagatccg
    16681 gcgggtttgt tgcccagcga actgcttgcc agcaagtcgt tcggcggcgt tttcaaggaa
    16741 ctgaccgagc gttttcatta cagtctgatc gatacgccac ccgttcacga agccgcagac
    16801 acaggagtga tcgcgccgtt gtgccattcg gtcatcatgg tcgtccgcat gaaccggacg
    16861 cccgagccgt tggtggatcg ctgcgtgaaa atgctgcagg ccaaccgtgt caacgtggcg
    16921 gggtgcgttc tggcaggcta ccacgagacg cggatggcct cgctcagcca cgattattac
    16981 cagagcacgt cataggaggg agacttgtcc atcagcatca ccgagttgga tgagggcaca
    17041 accggtttgt gggacgccta cgtcgccggt catccggaga gtaccatctt tcacacgttg
    17101 atctggcggg atgccgtgac cgaggccttc gggcaccgca gtcattatct gatggccttt
    17161 cgcgacgggc gcccggcggg cgtgtttccg ctgatgaggg tggacagccg gctggcgggc
    17221 acgctgatgg tgagcgtgcc ttatgcggtc tacggcggtt cgttggccga cgacgatgag
    17281 acacatcgcg ctctcctgga ggcggcgacg gcgttggcga tccaggccgg ggcgcagtgg
    17341 ctggatatcc gctccattcg cgcgcgatgg ccggaactgc cgatctcgga tcgatatgtg
    17401 acctttcgca agtctctgcc ggcagacccg aaccgggttc tggcggagat gccgcgcaag
    17461 gcccgagccg ccgcccggca ggcccgcgag cggttcaatc tggtttcgcg attcgacgag
    17521 tcgcgtcttg acgaggtttg gctgctctac agccagtcga tgcgccgcct ggcgtcgccg
    17581 aattatccat tgaggttttt ccgtgcattg atggacgcga cgcgcagccg gttgcaggcc
    17641 ggggagcggg tcgagcatct ggttcagatc gtggagcatc aaggcagggc cattgccgga
    17701 ctggtcagct tcgtctatcg cgacacgttg tcgccctatt ttgccggttg tgacgaacga
    17761 ttcgagaagt atcaccccaa tcatttcatg tacatgtgtg ccatggaaaa gggtgtggaa
    17821 cggggttgcc gggagttcga cttcgggcgg tcgcgggtcg ataacaaagg ttcctgcgat
    17881 ttcaagcgtt tccagggctt cgagccgacg ccgctgcaat accaatactt tgtgccggcg
    17941 ggcggccggg cgcctgatct gacgccgaac aacccgagat tgcagttaag tcggcgggtg
    18001 tggcccaagc tgccgctggc ggtgacccgg ccgctggggg cgtggttgtc gaagtcgatt
    18061 ccggggtgag ttggaaggtt gtccatgatc cgttttcaga ggtcggccgg acaggcagcg
    18121 ctgatgagcg agagagaccc cgccttctgg gcattacagg ggattgaaaa cgccgaacga
    18181 ctaagaaccg acaaatgagg attgacgact gcgatgaagc tgctgtacct ggtccatcgg
    18241 ttgccctgtc ccgcggacgg cggggcgaag cttcgcgccg ccgcacaact gcggtttc
//
LOCUS       DAOTPW010000108        18290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3079_length_18290_cov_6.215136, whole genome shotgun sequence.
ACCESSION   DAOTPW010000108 DAOTPW010000000
VERSION     DAOTPW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18290
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3079_length_18290_cov_6.215136"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="P5316_11255"
     CDS             complement(<1..1309)
                     /locus_tag="P5316_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85576.1"
                     /translation="MRNALLCLVGSAALVVLSIPLARTFFLQMEMIVPPRAGSHAAKS
                     LSAAAASFGDELQRSAQIMQLWRTLERLSSPVPRPNDRALLADLEPTNGLRAWLELLL
                     ILRREDEFSTTRPADADRSIMAALGGIRVAPPVRLYVMQELSLAHGALTKVSPRQRAA
                     YAAYLLYASPEDRQPNTSRSSRMVLTELVRRLESLAEQWQEKGKHTQAQTAREAIIRL
                     MTEAVTDSPTPEWAMLASELMIPNLRTLGANEQADRLEAFRQSLRAALQGDQINIIPH
                     TGRAVMAEKAHDRLMRSMTATLVTMAAWAILAAVALFLMVAILVTQPPDEITVQWRHL
                     KHGSREAAVLACSPMLGFLVLIALADIPWTWLVSYPTSKAALILPGLLLISTGAATWI
                     SIRLSEPFKPCPLPGKAAWAAAAVCIPTLVVLAVFVAPSGESWQ"
     gene            complement(1401..1862)
                     /locus_tag="P5316_11260"
     CDS             complement(1401..1862)
                     /locus_tag="P5316_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRR85577.1"
                     /translation="MTPEEKLKDLGYPLPPAPAPVGSYIPAVRIGNLVTTSGQLPMRD
                     GRLASTGKIGGKLSIEQGAEAARVAVVNALAQLAVVAGGLSNVKRIVRLGVFVNSADG
                     FVEQPKVANGASDLLVAVFGEAGRHVRAAVGSNELPLDAPVEIELTAELKQ"
     gene            complement(1859..2866)
                     /locus_tag="P5316_11265"
     CDS             complement(1859..2866)
                     /locus_tag="P5316_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRR85578.1"
                     /translation="MQNYRVQLDTYSGPLDLLLYLIRREEVDIYDIPISRILDQYIQY
                     VRVLEVMDPDGVGDFLVMAATLMEIKSRMLLPKPPPEVGDEDLLDPRADLVRQLLAYR
                     AFRDAAGLLGKRADEHAKRFGRPKLRLPTDGENDVDIEDVQIWDLLTAFNKLLASVGG
                     RRGVHEVIYDDTPVTLHAADIQDRLQRDGPSMPFEKIFEGRTRSEMIGLFLALLELIR
                     QDRVRIEQNEIFGPIFVHLIDPTPITQVMESAKEHGFKEYGLDEEEKQEAEEAPFEEI
                     EPGNEGLEPVFEDEERELAEEDDEYSRRINAVQISDIDLGRSLDEQAADADGPEGEPG
                     P"
     gene            complement(2928..4055)
                     /locus_tag="P5316_11270"
     CDS             complement(2928..4055)
                     /locus_tag="P5316_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85579.1"
                     /translation="METDRPLGVAILGFGFIGKVHAYAHLNIPLFYDPPPLRTRLAGV
                     ATRSSQSAAKAKSLMSFEEATTDQLSLINREDVDIVHICTPNDLHREALLAAIRAGKH
                     IYCDKPLTATLEEAEEVAAALKTYKATGQMTFNYRFLPATMRAKQLVDEGRLGQISHF
                     RGAYLHSGNIDRSRSLNWKADASRGGGVLNDLGSHVIDLLWHLLGPLEPMHAVTRVWA
                     RQRFSASEPSQRIVHVAEDFVMVTMRTRDGAPGVVEASKIATGSEDELRFEIHGEKGA
                     LRFNLMEPNWLEFCDLADPEAPLGGERGWRRVATVSRYPKPGGFPSPKNTLGWERSHM
                     HCVFSFLEAIAKGQPGNPDLRHGVEIQRVLGRIAKMATPVG"
     gene            4399..6063
                     /locus_tag="P5316_11275"
     CDS             4399..6063
                     /locus_tag="P5316_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85580.1"
                     /translation="MPRSSFQRAVPLFVALWLLSGSVVRAESMHVFGIHFWDWGANVD
                     VMSHRTGWVVECNALHTGAMPNVGGRYEPAVAEGFTILQRLDWGDTLDTVMPVTEADQ
                     NTFAQRCGNWANALKNYCRVYTIGNEMEFVTGMNPSVYASGFTKVRNAILAVQPDAKV
                     IIGHWNDSENVRAVIRLVGPDGYDGITDHTGSSVPHGFLDMLDQENARPGVGVYITEW
                     GWVADTNPNAMSVMRSFYLAIGASNASRARQVYCACWYLYPDFLGKTFSLELATLYDN
                     AAFEAATAIGTSFNSYSANPVIMSDLFADIPDAGTTVPVSWNTNVAARRQLWWTPVGT
                     WGWQNERYTPFSTAEGTVHQLTMSSLSPATAYEVSPLSTKKDYADAGGRRFRVKSGPW
                     PSQAEQSGAGRVTVQWATDWPTDSLVEYGPTTALGSWASSAGLVVNHQIVISGLSTGQ
                     YCYRVLSSEPNPDVNGARMYMRSPIRTFTIRHLVPGDFDEDYDVDQVDFGFFQACYSG
                     AGVEQGRPECNPARLDSDNDVDSADFALFLGCLSGPGINADPDCAS"
     gene            6178..7152
                     /locus_tag="P5316_11280"
     CDS             6178..7152
                     /locus_tag="P5316_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85581.1"
                     /translation="MRRMIAAPPDFDFRTAVCSHGFFVLAPNAWDPAKQSLRTVVTLD
                     EDKAVSVVIRERRAGSKADVSGNGLVVSCPAGLSSPQRTTVLRAVCRMLRLNEDLSGF
                     HELCRASKTHRQAAEMRFGRLLRSATLFEDVVKVICTCNTSWAQTVAMVRNITDLWGV
                     PAKGTTDRGFPTPARLAEVSPATLRKKARVGYRADFLHRLACDVVEGEVDLEAIESFD
                     GSSDELYEIVQSIHGIGDYAAAHLCMLLGHYDRLAVDTELKRFLTERHPGKRFTPASL
                     RMYYDRWEPYQFLAYWYELWSDYTHRHGQSHQWEPARIGKRITSIVQK"
     gene            complement(7270..8697)
                     /locus_tag="P5316_11285"
     CDS             complement(7270..8697)
                     /locus_tag="P5316_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRR85582.1"
                     /translation="MGKPTGFLEYHREEIDHRPVFQRVKDYAEFDIPHPEPRLIRQAA
                     RCMDCGIPFCHSAGCPVFNLIPEFNDLVYKNRWRDASENLHSTNNFPEITGRVCPAPC
                     EPACTLSINSDPVLIKHIEYQIAERAFAEGWVKPLAASRRSGRRIAVVGSGPAGLAAA
                     QQLARAGHDVTVFEKDDRIGGLLRYGIPDFKLEKSVIDRRLKQLAAEGVKFETGVVVG
                     QDISTRYLRKMFHAICLAMGAGRPRPLDVPGAEFKNVHYAMDFLTQQNRRNAGDFSPE
                     LHTNAITAKDKIVVVIGGGDTGSDCIGTALRQGAKEVHQFEILPKPPEKVNPRTPWPM
                     WPQILRTSSSQEEGCQRRWSVLTKKLTGRDNMATELHGCEVEWVPGANGMEMREKPGT
                     SFTMRVDLVLLAMGFVHVVHEGLVSQLGLKLDPRGNIVVNDYMTSQEGVFAAGDAHLG
                     ASLVVRAISTGRQAAAAIDRWLQER"
     gene            complement(8690..13237)
                     /gene="gltB"
                     /locus_tag="P5316_11290"
     CDS             complement(8690..13237)
                     /gene="gltB"
                     /locus_tag="P5316_11290"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRR85583.1"
                     /translation="MPDKRGLYDPAHEHDACGIGAVANISGRRDHSIIEYGRQVLMNL
                     IHRGAAGADESTGDGAGILIQIPHEFLAAQTDRLGFQLPGPLKYGAAMVFEPRDRNLR
                     SACEDILCEAVDRQGLRVLGWRDVPTDNSTLGPLAKSSEPVIRQLFVDGAELDPETLE
                     RRLYMARKRAERRVREQLGSAAEDFYIPSMSCRTMVYKGMFLAPQLFAYYPDLADEQT
                     ISALAIVHQRYSTNTFPSWRLAQPFRLCAHNGEINTLSGNRNKMRARERQLASPLLGD
                     DLSDLLPVLTPGGSDSMCFDNALELLTRAGRCLPHAMMMLIPEAFGPRYHISTDKRAF
                     YEYHASIMEPWDGPAAMVFTDGRLVGGTLDRNGLRPARYVITTNGLMILASETGVIDF
                     PIERIQAKGRLRPGRMFLADLEQGRIIVDNEIKGKIARQQPYRRWLEENRIELRGLFS
                     APEAPEIDPDTLASRLRTFGYTREDFQMILVPMAKDGQEPVGSMGTDTPLAVLSDRPK
                     LLFNYFKQLFAQVTNPPIDPLREGLVMSLMSFTGKKRNLLAETPEHCRQLKLTHPILT
                     NEDIRHLREVHRDDLRVATIPAVFEVDYVEPGRALQRGITNMVRAAASAIHEGASLLI
                     ASDREISPERAPIPSLLATAALHHGLLDMGLRGEAGLVIESGEPREVMHFCLLSGYGA
                     DAINPYLALEAIHYAKQRGEFPPETDVGHLVDKYVTAVKKGILKTISKMGISTLRSYQ
                     AAQLFEAIGLNHEVVESYFKGTASRIEGVGLDVIALEALARHKQGFQPRPAGALELDF
                     GGEYHHRLDGEEHLWNPTTVSRLQYAVRNNDPKAYAEYAKAINDQSRKLATLRGLFEF
                     VPGKPVPIEEVEPASEIVKRFCTGAMSHGSISKEAHETLAIAMNRLGAMNNTGEGGED
                     PARYKPLLSGDSANSGIKQVASARFGVTIEYLANAKMLQIKMAQGAKPGEGGQLPGHK
                     VTEEIARLRHSTPGVSLISPPPHHDIYSIEDLKQLIYDLKCSNPGVQVSVKLVAEVGV
                     GTVAAGVAKGNADEVLISGHDGGTGASPLSSIKHAGIPWELGLAETQQTLVLNGLRDR
                     ILVQVDGQMKTGRDVAIAALLGAERFGFGTAALVSLGCILMRKCHLGTCPVGVATQDK
                     VLRGRFTGQPEHLERFMLFVAEELRQIMAQLGFRKVEDMVGRVDRLSVRKGLDHWKAR
                     GLDFSAVFAKPDTSDGRPLRCVRPQPETLMDHLDWEIIRQAGAAIEKGQKVSLEMPIR
                     NVHRTVGAILSNRVYKAHGAKGLPDDTIEITFRGSAGQSFGAFLAPGVTLRLIGDSND
                     YLGKGLSGGRIIVQTPPGATYAPHENIIVGNTLLYGATRGEVFVNGVAGERFAVRNSG
                     ATAVVEGVGDHGCEYMTGGTVVVLGTTGQNFAAGMSGGVAYVLDEHQLFDTLCNLDMV
                     DLESIWQDADQKLLKDLIERHYHWTGSRQARRILDKWEEMAGRFVKVMPIDYRKALER
                     LREQEQARIEYTPATEEVFRG"
     gene            complement(13627..15807)
                     /locus_tag="P5316_11295"
     CDS             complement(13627..15807)
                     /locus_tag="P5316_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRR85584.1"
                     /translation="MSGSATRLQAIAAVTNYRPISAPLNFAETKPGEIFGSNVFSTTE
                     MRKRLPKEVYKSVKRTIDTGAKLDPSVADVVAAAMRDWAIEKGATHYAHVFFPLTGLT
                     AEKHDCFLQPDGQGGAIAEFTGKALIQGEPDASSFPSGGIRATFEARGYTAWDVTSPA
                     YILENPNGTTLCIPTAFVSWTGEALDKKTPVLRSMQALNTQAQRILKLFGHADGGMVS
                     STAGAEQEYFLIDRNFFFARPDLINAGRTLFGGKPPKGQEFEDHYFGAIPERVLAFML
                     ETERELFKLGIPVKTRHNEVAPAQYEIAPTFESANVATDHQQLIMITLKRVAEKYGMA
                     CLLHEKPFAGINGSGKHLNYSLGSAGLGNLLDPGDTPHANAQFLVFCAAIIRAVDKHA
                     KLLRAVVATAANDHRLGANEAPPAIISIYLGDQLTEIFEQIKKGEVKASKKKDYLHVG
                     VDVLPPLPKDAGDRNRTSPFAFTGNRFEFRAVGSSQSIAGPLVALNTIIADSLDFIAT
                     RLEKETKGDPKKLHGAVQAVIKDIMTEHGRVIFNGDNYSEEWHKEAARRGLPNLRTTI
                     EALPALLDKEVIEMFTRHKVLSKRELQSRYEIYLEQYVKSVMLEANLTLKIGKTQIFP
                     AAIRYQNELAQTCTNLKIVGYTFDTDTLDKMTALVKELQDTLAALEKIVAGNCHDKEA
                     EAENACHKVLPAMAAVRKAADALEAMVADDLWPLPTYQEMLFIK"
     gene            complement(16122..16463)
                     /locus_tag="P5316_11300"
     CDS             complement(16122..16463)
                     /locus_tag="P5316_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011363006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRR85585.1"
                     /translation="MKLITAIIQEEMLDRVREELTAEEVYRITVTRVSGRGQQVGEEI
                     YRGQRIVPNLLPKVRIDIGVNEEWVERTIKAIIKGARANGGSVGDGKIFVTPLLECIR
                     IRTGERGGQAI"
     gene            complement(16480..17766)
                     /locus_tag="P5316_11305"
     CDS             complement(16480..17766)
                     /locus_tag="P5316_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRR85586.1"
                     /translation="MKRRVIGLAPVLVLLSTGPAWGAEASEVDTGATAWMLTSTALVL
                     LMIPGLAMFYGGLVRSKNVLGTMMHSFAAMGVISVLWVVCGYMMGFGKGAGWCGWDPG
                     LMLLKSIDDKIMEQYRVPEYVFAMFQGKFAIITPALIAGAFAERVRFRTYCVFIAVWG
                     LLVYNPLCHWVWGADGFLYKMQAIDFAGGTVVHISSGVSGLVAALYLGARRGYPQNAF
                     HPSNLVMTLMGAGLLWVGWFGFNAGSAVSSSLATAQALTATQTAAAAGALTWIIVEGL
                     HQGKATALGFASGILAGLVAVTPAAGVVQPGGALALGAIAACVCYCAILVKNRLGYDD
                     SLDAFGIHGVGGFTGAIMLTFFIREGSQVRPMLSQFGVQFLAAGIAVAYAAALTLVIV
                     IVLEKLLGFRAEAHKEMAGLDHAFHGERGYGMLTAS"
BASE COUNT         3284 a         5773 c         5515 g         3718 t
ORIGIN      
        1 gttgccagga ttccccgctc ggagcaacga acaccgccag caccaccagc gtcggtatgc
       61 agacggcggc ggccgcccaa gccgccttgc cgggcagcgg gcagggtttg aacggctcgc
      121 tgagccggat agagatccag gtggcggcgc cggttgaaat caacagcaga ccgggcagga
      181 tcagtgccgc cttcgacgtc gggtaagaca ccagccacgt ccacggaatg tcggccagag
      241 cgatgagcac caggaacccg agcatgggac tgcaggcgag gacggcggct tcgcgcgacc
      301 cgtgcttgag atgtcgccat tggacggtga tttcatcggg cggctgcgtg accagaatcg
      361 ccaccatcag gaacagcgcg actgcggcca gaatcgccca ggcggccatg gtcaccagag
      421 tcgcggtcat tgatcgcatc agacgatcgt gggctttctc agccatgacc gcacgcccgg
      481 tgtgcgggat gatattgatc tggtctccct gaagcgccgc tcgcagggat tgccgaaaag
      541 cttcgagccg atccgcctgt tcgtttgcac cgagagtccg caagttgggg atcatgagtt
      601 ccgaggccag catggcccat tcaggcgtcg gtgagtcagt gaccgcctcg gtcatcaggc
      661 gaatgatcgc ctcccgggcc gtttgggcct gggtatgctt gcccttttcc tgccattgct
      721 cggcaaggga ttcgagtcgc ctgacaagtt ccgtcaagac cattcggctg gagcgggagg
      781 tgttgggctg cctgtcctcc ggcgaggcgt ataggaggta ggcggcatag gctgcccgtt
      841 gtcttggcga caccttggtc agcgccccat gggccaagct gagttcctgc atgacgtaca
      901 gacgaacagg cggggcgacg cggataccgc cgagggcggc catgatcgac ctgtccgcgt
      961 cggcaggccg ggttgtggag aactcgtctt cgcgtcgcag gatcaacaac agttcgagcc
     1021 aggcgcgcag cccgttggtt ggctccagat ccgcaaggag ggcccgatcg ttcggtcgag
     1081 gcaccggact ggacaacctt tcaagggttc gccagagctg catgatctgc gcggatctct
     1141 gcagctcatc gccaaacgat gcggccgcgg cggacaggga cttggccgcg tgcgatccgg
     1201 ctcgaggcgg gacaatcatc tccatctgta agaagaacgt tcgcgccagg gggatgctca
     1261 ggacaacgag tgccgcgctg ccgaccagac agagcagtgc atttcgcacc cgcagtgtgc
     1321 cgggcggtgg ctgacgaggt cttgacttgc tgtacggtct ggatcggccg atgggaacac
     1381 ctcagccgga gtcgccggcg ctactgtttc agttccgcag tcagttcaat ctcgaccggt
     1441 gcgtcgagcg gaagctcatt ggagcccacg gctgctcgga cgtgccggcc ggcctcgccg
     1501 aacacggcca cgagcagatc actggccccg ttggccactt ttggctgctc gacaaaaccg
     1561 tcggcgctgt tgacgaacac gcccagtctg acgatccgtt tgacgttcga caggccgccg
     1621 gccacgacgg ccaactgtgc cagggcgttg accactgcca cacgtgccgc ctcggcaccc
     1681 tgttcgatcg acagcttgcc gccgatcttg ccggtcgaag ccagcctgcc gtcgcgcatg
     1741 ggcagttggc cgctcgtcgt caccagatta ccaatgcgga cggcgggaat gtacgagccg
     1801 acgggagcgg gtgccggcgg cagcggataa cctaagtctt tcagcttttc ctcgggtgtc
     1861 acgggccggg ctctccttcc ggtccatcgg cgtctgcggc ctgttcatca agagaccggc
     1921 caaggtcgat atcgctgatc tgcacggcgt tgattcggcg gctgtactcg tcatcttctt
     1981 ctgcgagctc gcgttcctca tcctcaaaga cgggctccaa cccctcgttt ccgggctcga
     2041 tctcttcaaa gggggcttct tcggcttctt gcttctcttc ctcatccagg ccgtactcct
     2101 tgaaaccgtg ttccttggcc gattccatca cctgggtgat cggcgtgggg tcgatcaggt
     2161 gcacaaagat ggggccgaag atctcgtttt gctcgatgcg gacgcgatcc tggcggatca
     2221 gctccaacag ggccaggaag aggccgatca tttcactgcg ggtgcggcct tcgaagatct
     2281 tctcgaacgg catggacggc ccgtcccgct ggagccggtc ctgaatatcg gcggcgtgca
     2341 gggtgacagg cgtatcgtca taaatgacct cgtgtacgcc ccgcctcccg ccgaccgagg
     2401 ccagcagctt gttgaacgcc gtgagcaggt cccagatctg gacatcctcg atgtcaacgt
     2461 cattctcgcc gtcggtgggc agtcgcagct tcggccgccc gaatcgtttc gcatgctcat
     2521 cagcccgttt gccgagcagg ccggccgcgt cgcgaaacgc ccgatacgcc agcagttgcc
     2581 gcaccagatc agcccgcggg tcgagcaggt cttcgtctcc cacctcaggc ggcggcttgg
     2641 gcagcagcat gcgcgacttg atctccatca gcgtcgccgc catcaccagg aaatcgccca
     2701 ccccgtcggg atccatgacc tctagcaccc gcacatactg gatgtactga tccaggatcc
     2761 gcgaaatcgg gatgtcgtaa atgtcgactt cttcccgacg aataaggtaa agcagcaggt
     2821 ccaatggccc gctgtaggtg tcgagttgaa cgcgataatt ctgcatcggc aatcattgta
     2881 ccggcgattt cttggccgga aagtcgatca tcccgggtca accggcttca acccaccggc
     2941 gttgccattt tcgcgatccg acccaggact cgctgaatct cgacgccgtg tcgaaggtcg
     3001 gggttgcccg gttgcccttt ggcgatcgcc tccaggaagc tgaacacgca gtgcatgtgg
     3061 ctccgctccc agccgagggt gttcttgggg ctgggaaaac cgcctggttt cggatagcgg
     3121 ctcacggtgg cgactcgcct ccagccccgc tcgcccccca gcggtgcctc cggatcagcc
     3181 aaatcacaga actccagcca gttcggctcc atcaggttga accgcagtgc ccccttttcc
     3241 ccgtgtatct cgaaccgcag ctcgtcctcg gacccggtgg cgattttgct ggcctcgacc
     3301 acccccgggg ctccgtccct tgtccgcatc gtgaccatga cgaagtcctc agccacgtga
     3361 acgattctct gagacggctc ggaggcactg aaccgctgcc gggcccagac ccgggtcacc
     3421 gcgtgcatgg gctcgagggg ccctagcaga tgccagagca gatcgatcac gtgcgaaccc
     3481 aggtcgttaa gcacgccgcc ccccctggag gcgtcggcct tccaattgag tgacctgctg
     3541 cggtcgatgt tcccggagtg gaggtaggcc ccgcggaaat ggctgatctg cccaagccgg
     3601 ccttcgtcca cgagctgttt ggctctcatc gtcgccggca ggaaacggta gttgaaggtc
     3661 atctggccgg tcgctttgta tgtcttcaga gcagccgcga cttcctcggc ctcctccagc
     3721 gtcgccgtga gcggcttgtc gcagtagatg tgcttgcctg cccggatggc tgccagcagg
     3781 gcctcgcggt gcaggtcgtt gggcgtgcag atgtggacga tatccacatc ctcccgattg
     3841 atcagcgaaa gctggtcggt ggttgcctcc tcgaaactca tcaaggattt ggccttggcc
     3901 gccgactgac tgctccgggt tgctactcct gccagacggg ttcgcagtgg cggcggatca
     3961 tagaacaatg gaatgttcag gtgtgcatac gcatgcacct tgccgatgaa accgaagcca
     4021 agaatcgcca cgccgagcgg tcgatcggtc tccatgttct gtcttcccaa gagcttgtcg
     4081 cccagactcc gccgacccgt ccggcgggct ggttcggccg gatgatacga caatccccgg
     4141 ctaccagcca atcaacctgc gccgcatcat gcacagtggt caggttcctg ctgttgcgcc
     4201 gagaaggtgt ctggttatcc ctaacctgtt gtcagagcat aggataaagt gtttcctgtt
     4261 cagggacgca tctttctgtg aattgtgggt tctcttcgat cgggtgctca tgttatactg
     4321 aagccagtag ttgcggactt acaggttggt ccccccgcgg ttcagccgat atcagcgcga
     4381 gttgttggag gcaccgatat gccgcgatca tcatttcagc gggccgtgcc gctttttgtc
     4441 gcactctggc tgttgtcggg ttcggtcgtc agagccgagt ccatgcacgt ctttggcatc
     4501 catttctggg attggggcgc caacgtggac gtgatgagtc atcgaacggg ctgggtggtg
     4561 gaatgcaatg ccttgcacac gggggccatg cccaatgtcg gcggtcgtta cgaaccggca
     4621 gtagccgagg ggtttaccat cctgcagcgt ctggattggg gtgacaccct ggacacggtg
     4681 atgcccgtta ccgaagccga tcagaacacg tttgcccagc ggtgcggcaa ctgggccaac
     4741 gcgctcaaga actactgcag ggtatacact atcggaaacg agatggaatt cgtcactgga
     4801 atgaacccgt ctgtctacgc atccggcttt accaaggtcc gaaacgcgat tctggccgtc
     4861 cagccggacg ccaaggtgat catcggtcac tggaacgaca gcgaaaacgt gcgtgcggtt
     4921 atccggctcg tggggccgga cggctatgac ggcatcaccg accacaccgg cagcagcgtt
     4981 ccccacgggt tcctggacat gctcgatcag gaaaacgccc gccccggcgt cggcgtctac
     5041 atcaccgagt ggggctgggt cgccgacacg aacccgaacg ccatgtccgt catgcgaagc
     5101 ttctacctgg caatcggcgc ctccaacgcg agccgcgctc gccaagtcta ttgcgcctgc
     5161 tggtatctct atcctgactt tctgggaaag acgttcagcc tcgaactggc cacgctgtat
     5221 gacaacgccg cttttgaagc cgccacagcc atcggcacct ccttcaactc ctactcggcc
     5281 aaccccgtaa tcatgagcga cctctttgcg gacataccgg atgccggcac gaccgtgccg
     5341 gtaagttgga acacgaacgt tgcggcccgc cgccaactat ggtggacgcc ggtcggcacc
     5401 tggggctggc agaatgaacg ttacacaccg ttcagtacag cagagggcac cgttcatcag
     5461 ctcaccatga gttctctgtc ccccgccacg gcctatgagg tcagcccgct gtcgacgaag
     5521 aaggactacg ccgacgcggg cggcaggcga ttccgggtca agagtggccc atggccttcg
     5581 caggccgagc agagcggcgc cggtcgagtg accgtgcaat gggcgacgga ctggccaacc
     5641 gacagcctcg tcgagtatgg ccccacaacc gccttgggct cttgggcaag ttcggcgggg
     5701 ctggtcgtca atcatcagat cgtcatcagc ggcctttcca ccgggcaata ctgctaccgt
     5761 gtgctgtcca gcgaacccaa ccctgacgtg aacggtgctc gaatgtacat gcgctctcca
     5821 atccgcacgt ttacgatcag gcacctggtt cctggtgatt ttgatgagga ttacgatgtc
     5881 gatcaggtcg acttcggctt ctttcaggcc tgttacagcg gcgctggcgt cgagcaagga
     5941 agacctgagt gcaatcctgc ccgtttggac agtgacaatg acgtggattc ggccgacttc
     6001 gccctgtttc tgggatgtct cagcggtcca ggcataaacg ccgaccccga ctgcgcaagc
     6061 tgacgatccg cacgaccggg aatcctttct cgatctccgg gtccccccgg cggccggccc
     6121 gccagtgctt cattcgcgag tttgcaggta ttcccgtgtc gttgataatt ccccggcatg
     6181 agacgcatga tcgccgcgcc gccggacttc gatttccgca cggccgtttg ctcgcacggt
     6241 ttcttcgtgc tcgcacccaa tgcctgggac ccggccaagc agtctttgcg tacggttgtc
     6301 acgctcgacg aggataaggc cgtttcggtc gtgatccgcg agaggagggc cggttcgaag
     6361 gcagacgtat cgggcaacgg cctggtcgtc tcttgtcctg cgggcctgtc ctcgccgcag
     6421 cggacgaccg tattgagggc cgtgtgccgc atgctgcggc tcaatgagga tctctcgggg
     6481 ttccacgagc tttgccgggc atcaaagact caccgccagg cggccgagat gcgtttcggc
     6541 cggctgttgc gcagtgccac gctctttgag gatgtagtca aggtcatctg tacgtgcaac
     6601 acctcgtggg cccagacggt cgcgatggtg cgcaatatca ccgatctctg gggagtcccc
     6661 gccaaaggca ccaccgatcg cggtttcccg acgcccgctc gcctagcaga ggtttcgccg
     6721 gcaacgttga ggaaaaaagc ccgcgtcggg tatcgtgcgg atttcctgca tcggctggcc
     6781 tgcgacgtcg tcgagggtga ggttgacctg gaggccatcg agtccttcga cggctcaagc
     6841 gacgaactgt acgagatcgt gcaatcaatt catggcatcg gcgactacgc tgccgcccac
     6901 ttgtgcatgc tgctgggaca ctacgaccgg cttgcggtgg acacggaact gaagcgtttc
     6961 ctgaccgaac gtcaccctgg gaaacgcttt acccccgcct cgctccgcat gtactatgac
     7021 cgctgggagc cttaccagtt cctggcctac tggtatgaac tatggagcga ctacacccac
     7081 cgccacggcc agtcccatca gtgggagccc gcacggatcg gcaagcggat cacctcgatc
     7141 gtgcagaagt aagagcccaa ggcgaaccgc ctttttgcgg gccgcaaccc aagcgctctg
     7201 cgatccagaa acatgcgcgc aagccgcaca catatccggc cgtaaggttc taagaggata
     7261 ccagcctgct taccgctcct ggagccatcg gtcgattgcc gctgcggctt ggcgaccggt
     7321 gctgatggcc cgaaccacaa gggaggcacc gaggtgggca tcgccggccg cgaacacgcc
     7381 ctcttggctg gtcatgtagt cgttgaccac gatgtttccg cgcgggtcga gcttgagccc
     7441 gagttgactg accagtcctt catggaccac gtgaacaaac cccatggcca gcagcacgag
     7501 atcgacccgc atggtaaagc tcgtaccggg cttctcgcgc atctccatgc cgttggcgcc
     7561 gggcacccat tcgacttcgc agccgtgtag ttcggtcgcc atgttgtcgc ggccggtcag
     7621 cttcttggtc agcacactcc accgccgctg acaaccttct tcctgcgagg acgaagtccg
     7681 caggatctgg ggccacatcg gccagggcgt gcgcgggttc accttttccg gcggtttcgg
     7741 cagaatctca aactggtgaa cctccttggc tccctgtcgc agagccgtgc cgatgcagtc
     7801 gctgcccgtg tcgccgccgc cgataaccac cacgatcttg tccttggcgg tgatggcgtt
     7861 ggtgtgcagt tcaggcgaga aatcgcccgc gttgcggcga ttctgctggg tgaggaagtc
     7921 catcgcgtaa tggacgtttt tgaactctgc gcccggcacg tcgagaggcc gaggccggcc
     7981 ggcccccatt gccaggcaga tcgcgtggaa catcttgcgc agatagcgcg tggaaatgtc
     8041 ctgtcccacc accacgccgg tctcgaattt cacgccttcg gcggccaact gcttgagccg
     8101 gcgatcgatg accgactttt cgagcttgaa atcgggaatc ccgtaccgca gcaacccgcc
     8161 gatgcgatca tccttctcaa aaacggtcac atcatggccg gcgcgggcca gttgctgtgc
     8221 agccgccagg cccgccggac cggatccgac cactgcgatc ctgcggccgc tccgccggct
     8281 tgccgccaac ggcttcaccc agccttcggc gaacgcccgc tcggcgatct ggtactcgat
     8341 gtgcttgatg agcaccggat cgctgttgat gctgagcgtg caggccggct cgcagggcgc
     8401 cgggcacacg cggccggtga tctcggggaa gttgttcgtc gagtgcaggt tctcgctcgc
     8461 gtcccgccat ctattcttat agacgaggtc gttgaactcg gggatcagat tgaacaccgg
     8521 gcagcccgcg ctgtggcaga acggaatccc gcaatccatg caacgggcgg cctgccgaat
     8581 caggcgtggc tcagggtgag gaatgtcgaa ttccgcataa tccttgacgc gctgaaacac
     8641 ggggcgatgg tcgatttcct cgcgatggta ttccaggaat cccgtcggct tacccacgga
     8701 acacctcctc cgttgccggc gtgtactcga tccgcgcctg ttcctgttcg cgcaaccttt
     8761 ccagtgcctt tcggtagtcg atgggcatga ccttgacgaa ccgtccggcc atttcctccc
     8821 atttgtctag gatacgcctg gcttgccggc tgccggtcca atgatagtgt ctctcgataa
     8881 gatccttcag aagcttctgg tccgcatcct gccagatcga ttccaggtcg accatgtcga
     8941 ggttacagag cgtgtcaaaa agctggtgct catcaagcac gtaggcaacg ccaccgctca
     9001 tgcccgcggc gaagttctgg ccggtggtcc caaggacgac taccgttccc ccggtcatgt
     9061 actcgcagcc gtgatcgccg acgccttcca caacggccgt cgctccgctg ttgcggacgg
     9121 cgaatcgctc gccggcgacg ccgttgacaa aaacctctcc gcgcgtggct ccgtagagga
     9181 gtgtgttgcc cacgatgatg ttctcgtgcg gggcgtaggt cgcccccggc ggcgtctgaa
     9241 cgatgattcg cccccctgac aaacccttgc cgagatagtc gttcgaatcg ccgatcaggc
     9301 gcaaggtgac gcccggtgcc aggaatgcgc cgaagctctg cccggctgat ccgcgaaatg
     9361 tgatttcgat ggtgtcgtcc ggcaaaccct tggctccgtg agccttgtac acgcggttac
     9421 tgagaatcgc ccctactgtg cgatggacgt ttcgaatcgg catctcgagc gagaccttct
     9481 gtcccttttc gatggctgcg cccgcctgcc tgatgatttc ccagtcgaga tggtccatca
     9541 gcgtttccgg ctgcgggcga acgcaacgga ggggccggcc gtccgaggtg tcgggcttcg
     9601 cgaaaaccgc cgagaaatcg agccccctgg ccttccagtg gtcgaggcct ttgcggacgc
     9661 tcaggcgatc gacccgcccg accatgtcct cgaccttgcg gaagccgagc tgggccatga
     9721 tctgacgcag ctcctccgcg acaaaaagca tgaaccgctc gagatgctcg ggttggccgg
     9781 tgaatctgcc tctcaagacc ttgtcttgcg tggccacacc gaccgggcag gtgcccagat
     9841 gacatttgcg catcagtatg cagccaaggg agaccagcgc ggcggtgccg aagccgaagc
     9901 gctcggcgcc cagcagggcg gcaatggcga cgtcgcggcc ggttttcatt tgtccgtcga
     9961 cctgcacgag gattcggtcg cgcagcccgt tcagcaccag agtctgctgc gtctcggcca
    10021 gcccgagttc ccacgggatt cccgcgtgct tgatcgaaga cagcgggctg gctccggtgc
    10081 cgccgtcgtg gccgctgatg agcacttcgt cggcgtttcc tttggccacg cccgccgcaa
    10141 ccgtgcccac gccgacctcc gcgaccagct tcaccgacac ctgtacgccc gggttgctgc
    10201 acttgaggtc gtagatgagc tgcttgagat cctcgatcga gtagatgtcg tggtgcggcg
    10261 ggggtgaaat cagcgacaca cccggcgtcg agtgtcgcag ccgggcgatc tcctcggtca
    10321 ccttgtgtcc tggaagctgg ccgccctcgc cgggcttggc tccctgagcc atcttgatct
    10381 gaagcatctt ggcgtttgcc agatactcga tcgtcacgcc gaagcgtgcg ctggccacct
    10441 gcttgatgcc cgagtttgcc gagtcgccgc tcagcagcgg cttgtatcgg gcggggtctt
    10501 ctccgccttc gccggtgttg ttcattgccc ccagacgatt catggcgatc gcgagcgtct
    10561 cgtgggcctc cttgctgatg gagccgtgcg acatggcgcc ggtgcagaat cgtttgacga
    10621 tctcgctggc cggctcgacc tcctcgatcg ggacgggctt gccgggtacg aactcgaaga
    10681 ggcctcgcag cgtggccagc ttgcgcgact ggtcgttgat ggccttggca tattcggcgt
    10741 aggccttcgg atcgttgttt ctcaccgcgt actgcaatcg tgagacggtc gtggggttcc
    10801 agaggtgctc ctcgccgtcc agccggtggt gatactcgcc gccgaagtcc agttcgagcg
    10861 ccccggccgg acgcggctgg aacccttgtt tgtgtctggc aagagcctcc agggcgatca
    10921 cgtcgagccc gacgccctcg atgcgggagg cggtgccctt gaagtagctt tccaccacct
    10981 catgattcag cccgatcgct tcgaaaagct gagcagcctg gtagctgcgc agcgtcgaga
    11041 tgcccatctt cgagatggtt ttgagaatgc ctttcttaac ggcggtaacg tacttgtcca
    11101 ccaggtggcc gacatcggtt tcgggcggga attccccccg ttgcttggcg taatggatcg
    11161 cctccagagc caggtacggg ttgatcgcgt cggcgccgta acccgacagc agacagaaat
    11221 gcatgacctc gcgcggctcg ccgctttcga tcaccaggcc ggcttcgccg cgaagcccca
    11281 tgtccagcag accatggtgc agggctgccg tggccagcag cgacggaatg ggcgctcgct
    11341 ccggggatat ctcgcggtcg ctggcgatca gcagggaggc tccctcatgg atggcgctgg
    11401 cggccgcccg gaccatgttg gtaatgcccc gctgaagagc cctccctggc tcgacgtagt
    11461 ccacctcgaa aacggctgga atcgtcgcca cccggaggtc atcgcgatgg acctctcgca
    11521 aatgacgtat gtcctcgttg gtcagtatgg gatgagtcag cttcaactgt cggcaatgct
    11581 cgggcgtttc ggccagaagg ttgcgtttct tgcccgtgaa ggacatcagc gacatgacca
    11641 gcccttcccg gaggggatcg atcggcgggt tggtcacctg ggcaaacaac tgtttgaagt
    11701 agttgaagag cagcttgggc cggtccgaaa gcaccgcgag cggcgtgtcg gtacccatcg
    11761 atcccaccgg ctcctggccg tccttggcca tcggcacaag gatcatctgg aagtcttctc
    11821 gggtgtaacc gaaagtgcgc aaacgcgagg ccagggtatc gggatcgatt tccggcgcct
    11881 cgggagcgct gaacagcccg cgcaactcga ttcggttttc ttccagccag cgccggtagg
    11941 gctgctgccg ggctatcttg cccttgatct cgttgtcaac gatgatgcgc ccctgttcga
    12001 ggtcggcaag gaacatgcgg ccgggccgca gccgaccctt agcctggatt ctttctatgg
    12061 ggaagtcgat cacgccggtt tcgctggcca ggatcatcag gccattggta gtgatcacgt
    12121 agcgagccgg ccgtagaccg ttgcgatcca atgtgccgcc gaccagccgg ccatcggtga
    12181 agaccatcgc agccggtccg tcccacggtt ccatgatcga cgcgtgatac tcgtaaaaag
    12241 cccgcttatc ggtgctgatg tggtaccggg gtccgaacgc ctccggaatc agcatcatca
    12301 tcgcgtgcgg caggcagcgg ccggcgcggg tcagcaattc cagggcgttg tcgaaacaca
    12361 tcgagtcgct tccacccggg gtcaggaccg gtaagagatc gctcaggtcg tcacccagca
    12421 gcggactggc cagttgcctt tcgcgggccc gcatcttgtt gcggttgccg ctcaacgtgt
    12481 tgatttcgcc attgtgagcg cacaggcgaa acggttgggc cagtcgccag ctcgggaacg
    12541 tgttcgtact gtatcgctgg tgcacgatcg ccaaagcgga gatcgtttgc tcgtcggcca
    12601 gatccggata ataggcaaag agctggggag ccagaaacat tcccttgtaa accattgtcc
    12661 tgcacgacat cgagggaatg taaaaatcct ccgcggcgga accgagttgt tcgcgaactc
    12721 gtcgctcggc ccgcttgcgg gccatgtaca gccgccgttc gagcgtttca gggtccaatt
    12781 ccgctccgtc aacgaacaac tgccggatca ccggctcgct cgacttggcc aacggaccca
    12841 gcgtgctgtt gtccgtggga acgtctcgcc agcccaggac tctcaggccc tgtcggtcga
    12901 ccgcttcaca aagaatatcc tcgcatgcgg atcgaaggtt cctgtcgcga ggctcgaata
    12961 ccatggccgc cccgtacttc agcggtccgg gcagttggaa tccgagtcgg tcggtctgag
    13021 cggccagaaa ctcatgcgga atttgaatca ggatgcccgc gccatcgccc gtcgattcat
    13081 ccgccccggc ggctccgcgg tggatgaggt tcatgagaac ctgcctgccg tattcaatga
    13141 tcgaatgatc gcggcggccg gaaatattcg ctacggcgcc gattccacag gcgtcatgct
    13201 catgggccgg gtcgtacaaa ccccgcttat ctggcattcg cgacaacttc atttgcccgt
    13261 ttggctcctt tgtgtatagc cccgcgctcc gcggcccggc gggaccgcac cggaccggca
    13321 ttcagagcgg cgtattctaa gacattccga ccttccgggc gagcggacag gtttcgcctg
    13381 tcccttgggg ctgtgcggcc atccgtttgg tctcctcaaa tccccggcgc aaggacgaag
    13441 cctccattat ccgatcgccg ggcggtcgga caatcgaggc gctcttgcgt gttcggttgc
    13501 gacgcgctcg tttcagcgcg tccccttggt tcaggaagag ccgttcccaa gtacggcgcc
    13561 cgaggttgtc aggccccgga cgcgcgatta tcagaccaag cgtacttctt gcccgggtct
    13621 gcgactttac ttgatgaaca gcatctcctg ataggtcggc agcggccaaa gatcgtcggc
    13681 caccatggcc tccagcgcat cggccgcttt gcgaacagcc gccatcgccg gcaaaacctt
    13741 atgacaggcg ttctcggcct cggcctcctt gtcgtggcag ttgccagcca cgatcttctc
    13801 cagcgcagcc agtgtgtcct gcagttcctt caccagcgcg gtcatcttgt cgagcgtgtc
    13861 ggtgtcgaag gtgtagccga cgatcttcag gttcgtgcag gtctgtgcca gctcgttctg
    13921 atagcggatg gccgccggaa agatctgggt cttgccgatc ttcagcgtca ggttggcctc
    13981 cagcatcacg ctcttaacgt actgttcaag ataaatctcg tagcggctct gcaattctcg
    14041 cttcgaaagc accttgtggc gggtgaacat ctcgatcact tccttgtcca gcagggccgg
    14101 caaggcttcg atcgtggtcc gcaggtttgg cagacctcgc ctggcagcct ccttgtgcca
    14161 ttcctccgag tagttgtcgc cgttgaagat cacccttccg tgttcggtca tgatgtcttt
    14221 gatcaccgcc tgtactgcgc cgtgcagctt cttcgggtcg ccttttgtct ccttctccag
    14281 gcgcgtggcg atgaagtcca gcgagtcggc gatgatcgtg ttcagggcca ccagcgggcc
    14341 ggcgatcgat tgacttgatc cgaccgccct gaactcgaac cggttgccgg tgaacgcgaa
    14401 cgggctcgtc cgatttcgat cgcctgcatc cttgggcagg ggcggaagaa cgtccacacc
    14461 gacatgcaga tagtcttttt tcttcgatgc cttcacttcg cccttcttga tttgctcgaa
    14521 gatctccgtg agctgatcgc ccaggtagat cgaaatgatt gccggcggag cttcgtttgc
    14581 ccccaggcgg tgatcattgg ctgccgtggc caccaccgcc cgtagcagct tagcgtgttt
    14641 gtccacggcg cggatgatgg ccgcgcagaa caccagaaac tgagcattcg cgtgaggcgt
    14701 gtcgcccgga tccagtagat tgcccaggcc ggcactgccc agcgagtagt tcaggtgttt
    14761 gcctgagccg ttgatacccg caaacggttt ttcgtgcaga aggcaggcca tcccgtattt
    14821 ctcggccacg cgcttcagtg tgatcatgat cagttgttga tggtcggtgg ctacgttggc
    14881 ggactcgaag gtcggcgcga tctcgtattg ggccggggcg acctcgttgt gccgcgtctt
    14941 gaccgggatg ccgagcttga agagctcacg ctcggtttcc agcatgaagg ccagcacccg
    15001 ctccgggatc gctccgaagt agtggtcctc aaactcctgt cccttcggcg gcttgccgcc
    15061 gaacagcgtc cgacctgcgt tgatcagatc cggccgggca aagaaaaagt tgcgatcgat
    15121 gaggaagtac tcctgctcgg caccggcggt cgacgagacc atgcctccgt ccgcgtggcc
    15181 gaacaacttc aggatcctct gcgcctgggt gttcagcgcc tgcatcgagc gcagaacagg
    15241 cgtcttcttg tcaagggcct cccccgtcca tgacacgaac gccgtcggaa tgcacagcgt
    15301 ggtcccgttg ggattctcga gaatgtaagc cgggctggtc acgtcccacg ccgtgtagcc
    15361 gcgggcctcg aaagtcgccc ggatgccgcc ggacggaaag ctcgatgcgt ccggctcgcc
    15421 ctggatcaac gccttgcccg tgaactcggc gatggccccg ccttggccgt ccggttgcag
    15481 gaaacagtcg tgcttctcgg cggtcagccc cgtcaaggga aagaaaacgt gggcataatg
    15541 cgtcgcaccc ttttcaatcg cccagtcccg catcgctgcg gcaacgacat cggccacaga
    15601 agggtccaac ttggcccccg tgtcgatggt tctcttcact gacttataga cctctttcgg
    15661 aagtcgcttt ctcatctccg tcgtgctgaa cacgttactg ccgaagattt ctcccggttt
    15721 cgtctcggcg aagttcaaag gcgccgaaat gggtctgtag ttggtgacgg ccgcgattgc
    15781 ctgcaatcgg gttgcgcttc cgctcatggt ggtcctgttc tttccgttga tgccttactt
    15841 tctattcatg gccccggccg gtgccggctc gcagcaccga tcgtgccttc acatctttca
    15901 gatcgcggtt ccagcccgca gcacctctcc cgctttgtgc atggctcatg ccacagccgg
    15961 tttctaaagg caaaggaacc agccgggagg taaagctaag ggcgggcgac agttaaagca
    16021 attcttcttc cggaacgtac gctgcaaccg taccccaatg tagtttagtc gtccaatggg
    16081 ctacatttgt gtaggatcgt ctccgcggcc ggcgtctgct ctcagatggc ttgtccgccc
    16141 ctttcgccgg tacgaatccg gatgcactcc agcagcggag tcacgaagat cttgccgtcg
    16201 ccgaccgagc ccccgttggc ccgggcaccc ttgatgatgg ctttgatcgt gcgctccacc
    16261 cactcttcat tcacgccgat gtcgatacgt accttgggca gcagattggg cacgattcgc
    16321 tggcctcgat agatttcctc gccgacctgc tgcccgcggc cggatactcg tgtgaccgtg
    16381 atccggtaga cttcttcagc ggttaattcc tcgcggaccc gatccagcat ctcttcctga
    16441 atgatggcgg ttatcagctt catgttaacc tcgattcgct caactggcgg tgagcatgcc
    16501 atagccgcgc tcgccatgga aggcgtggtc cagaccggcc atctccttgt gcgcctcggc
    16561 ccggaatccc aacagttttt ccagaacgat aacgataacc agcgtcagcg ctgccgcgta
    16621 cgccaccgca atgccggcgg cgaggaattg aacgccgaac tgcgaaagca tcggacggac
    16681 ctgcgagcct tcacggatga agaaggtcag catgattgcc ccggtgaatc cgccgactcc
    16741 atggatgccg aacgcatcca ggctgtcgtc gtatcccaga cggttcttca ccaggatggc
    16801 gcaatagcat acgcaagcgg caattgcccc gagggccaat gccccgccgg gttgcacgac
    16861 ccccgctgcc ggcgtcaccg ccaccaagcc tgcgagaatg ccgctggcaa atcccaacgc
    16921 agtggctttg ccttggtgca ggccctccac gatgatccat gtcagcgctc cggccgccgc
    16981 tgccgtctga gtggcagtta acgcttgagc ggttgccaga ctgctcgaaa cggcgctgcc
    17041 ggcattgaaa ccgaaccagc ccacccacaa caggcccgca cccatcaggg tcattaccag
    17101 attgctggga tgaaacgcgt tctgcgggta tccgcgccgt gcaccgagat acaaggccgc
    17161 aaccagcccg ctgacgcccg aggaaatgtg aaccaccgtt ccgcccgcaa agtcaatcgc
    17221 ctgcatcttg tacagaaaac cgtcggcacc ccacacccag tggcacagcg ggttgtagac
    17281 cagcaggccc cacacggcga tgaaaacgca ataggtgcga aaccgcactc gctcggcgaa
    17341 tgccccggcg atcagtgcgg gggtgatgat ggcaaacttt ccctggaaca tcgcgaagac
    17401 gtattccggt acccggtact gttccatgat cttgtcgtcg atgctcttga gaagcatcaa
    17461 gccgggatcc cacccgcacc agccggcgcc ctttccgaaa cccatcatgt agccgcaaac
    17521 cacccacaaa acactgatca cgcccatggc cgcgaaactg tgcatcatgg tgcccaggac
    17581 gttcttggag cggaccaggc cgccgtagaa catggccaga ccggggatca tcagcagcac
    17641 cagcgcggtg gaggtgagca tccatgccgt ggcaccggta tctacttcgg atgcctcggc
    17701 accccatgcc ggtcccgttg acagaagcac cagtaccggt gccaatccga tcactcgtcg
    17761 tttcatcgtt tcctccgttc gcaccactac cggcagtcaa ccttcgccac agatcgtttt
    17821 gttccgagct ctgcggccgg tggacggcac ggttttcctg tcgttctcgg cgaggcaaac
    17881 cggcagacca cgcccgcgtc ggccctgcgc caaacgcgag gcgaatcgtc ttacgcggtg
    17941 ggcaattggc gtgccggatt cggacacagc gcggcttgtt gaatggcgca agtctttgtg
    18001 aaagaaggtg ttacagaaaa caattcatcg ccgacggatg gccagatcga atggtcaggc
    18061 atacgttacc gtagggaagc gcccaaaggg tatacgcaaa tgtaggaaca ggtttgccag
    18121 ccgcgtgcgt caccgacagg agaagaagcg acaggctgcc ggtgcggact gcctggtgca
    18181 gggggtcttt tcgcccctgc aagaaaaggg gcgggggttg gccggggcta ccacttggcg
    18241 cggaaccgag cagggtcgat gccgtgcttg tggatgcgca ggcccatgac
//
LOCUS       DAOTPW010000109        18148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3102_length_18148_cov_4.952578, whole genome shotgun sequence.
ACCESSION   DAOTPW010000109 DAOTPW010000000
VERSION     DAOTPW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18148
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3102_length_18148_cov_4.952578"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="P5316_11310"
     CDS             <1..1403
                     /locus_tag="P5316_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRR85587.1"
                     /translation="LGALAQEVTGNPDWMSSVPSWQTPIFKDYDSDYQFIFVLAVFYL
                     FRNILGGLGAGADPRYFGARSERECGLLTFFWTWLMAFRWPLMMGFAAMGLFLVKGMF
                     PNQEVLAQASQMVKRYVAEQAGARGPDALADKDLMKRLLPRERWEDKLSDISRHPEKY
                     PPLADSLRGVLGDGWQSKLDMVSYEGQINPERILPAVILQYLPMGLRGLMLVSLVAAA
                     MSTFSPTVNISTALFTRDIWQAFVRPKASNPELIAVSWMFGVLLTAGGFAMAYTSKNI
                     NDIWDWIIMGVTAGLAIPNLLRLYWWRFNAGGVVVGTFIAVVGAVAQRIIWPDMGPYL
                     KFGLNTSVSFAGCIIGTFLTKPTDPRVVEHFYRTTRPFGFWGPLKKLLPPDMRTEMDR
                     ENRRDILSLPFALGWQVTLFLLPMQLIIGAFRDFAITLVVFLVCLGWLLVVWYRNLPA
                     DDSPLTKANPVFPPAS"
     gene            complement(1543..4113)
                     /locus_tag="P5316_11315"
     CDS             complement(1543..4113)
                     /locus_tag="P5316_11315"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR85588.1"
                     /translation="MKSIANRFLLPTGVFVVLFTTFLLLRAYADRLDQAALLLKQQAE
                     LALEFDLAIRDYVGAQIRPEMEKRVAKDEFHPETMSTSFVARSIFERVRGRFPHYIIR
                     FSSDNPRNPVNKAGPDELKMIEYFDANPQVNNWSGEIRLDGRRYLAHFSARRMQESCL
                     RCHGEPEDAPASLIRRYGNVAGFHRPLGKVIALDTVAIPVDSAQSALKAHMVRDSMTM
                     LVGLAGMLGMIALVFRKTVSKRLTAIAQHFQLLATDPESREVQALQVTQQDEIGNLAE
                     SFNRLSERLRSDRELLEQRVAERTAELRKANVELAHEIAERKQAEKALQLNSFSVEHA
                     RDSIFWISEDARLFYVNEATCRRLGYSREELQSMTVHDIDPDFPPDRWRAHWQHMRSC
                     GFLEFESRHKHRDGSVFPVEISANYLEFDGQAYIFAFARDITERKIVEADLQHAKEAA
                     ETANQAKSEFLANMSHEIRTPMTAILGYVEELSKGCPYSCEFGDSYMREYTEIIARNA
                     EHLLLIINDILDLSKIEAGKIEVFRAPASLRRVIEDVESIMRVRAGEKGLPLSVECKE
                     TVPEVIRTDVVRLRQILINLLGNAVKFTDSGIVSLTASFMEDSGGNACICVDVSDTGI
                     GIGVEQQSRLFEPFTQVDSSSARRFGGTGLGLAISKRLAEMLGGTLTLLESAPGKGST
                     FRLTLPFDPVDEPEPSREASTAAQLAAEPAAGPNAGDLLNCNILLAEDTRDTQMLVVR
                     LLTKCGARVTAADNGREAVEKVMAAQAAVHPFDVVLMDGQMPVMDGLKATRMLRAKGY
                     TGVIIALTAHAMAEDKNKCIEAGCDDYATKPIKIAALRETIRRNLAKSRCLAQTSSDA
                     "
     gene            4527..4613
                     /locus_tag="P5316_11320"
     tRNA            4527..4613
                     /locus_tag="P5316_11320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4561..4563,aa:Ser,seq:cga)
     gene            complement(4879..5835)
                     /locus_tag="P5316_11325"
     CDS             complement(4879..5835)
                     /locus_tag="P5316_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR85589.1"
                     /translation="MAGGLLAGRSLQAAQDPATTLPVLQTRPDTGPIDSLLKSTVVHI
                     RRSEVVDRQTIHPPLVREMLEESLKVLTDTLRPADAWNRLFKPEDVIGIKFNQVGAKE
                     FNTTEAIAAQLVASLGDAGFSPARIMLIEVPAPLGASLGTRPEVTGWSGGEVAFDGGS
                     EELAAVLQEVTAIINVPFLKTHNIAGITGCLKNLSHALIRHPGRYHDHGCAPYVGNIV
                     ALPQIRNKLRLNIVNAIRALYKGGPNVRPDGTWDHAGLIVSQDPVAADAVGLEIINDR
                     RGRLGLASIGTGSGHVPHIRAAAELGLGTDDQDYIELREMAE"
     gene            6107..7552
                     /locus_tag="P5316_11330"
     CDS             6107..7552
                     /locus_tag="P5316_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HRR85590.1"
                     /translation="MNSPIIQSESEQAKAIQARLREANQTHLLAHWHQLDDQQRELLL
                     EDINRINLAGLSGLIKSHVMADRPFTLPRDIRPVVCYPARPGIDQVGLYADAVKRGVS
                     LIRRNKVAALTVAGGQGTRLGFDGPKGAFPISPVKSKPLFQLFAEFIRGTNRRYGADL
                     HWYIMTSPQNHADTVAFFEANHFFGLRPQRVRFFRQGIMPAFSMDGKILLDQKHRIAF
                     SPDGHGGSLLALRRSGMLAEMAEAGIEHLSYFQVDNPLVKAIDPLFIGLHDMTGSEMS
                     SKTIPKVDDLERVGNFVIGDGKAMVIEYSDLPDELAHARDANGNRLFDAGSIAIHLLS
                     RRFVERLTADEAHFGLPWHRAVKKVPYVDERGRRIEPEKPNAVKLESFIFDAIPLAGK
                     PLILQTIREEEFSPVKNVTGVDSAESSRRDLNRRAARWLESAGYAVPWREGEPDGLFE
                     ISPLLALDADHLREQMSTPPRITPGGAHYWE"
     gene            7764..9137
                     /locus_tag="P5316_11335"
     CDS             7764..9137
                     /locus_tag="P5316_11335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR85591.1"
                     /translation="MTDSSKGTLLVVDDEPLKRATLQIELAGAGYTVLEAADGAAAVK
                     LIETRPVDVVITDVRMPEMDGIQLLEAVKSRSPRTHVILMTAYGTVDSAVEAIKRGAY
                     DYINKPFATEALVEKLDRLLACRSAEGNGHGGPETLGQLVGQSYASRRLFEQIRAVAD
                     SDRTVLVEGEAGTCRQRVAATIHQLCKRSAGPLVRFNCTACVAEVLESELFGTGDEGA
                     AQRIGRLEEAAGGTLFLDEIEVLPMPLQARLLQAMDEGFFNRVGGGRVRLDARLVFGT
                     QENLRRKVEAGQFREDLYYRLDAVHLVIPPLRDRREDIPALAAHYLRHSAKAAEGKPV
                     PTRISTHAMDVLMSYHWPGNISELENVLERAVAFATGPEIQPRDILVPSAGQASQEVV
                     AQTTMHPGLTETIAGVEQSLIDAALRKAAGNQAKAAQFLGIPRTTLRDKMAKYGMVGK
                     PAKRELA"
     gene            complement(9281..9748)
                     /gene="smpB"
                     /locus_tag="P5316_11340"
     CDS             complement(9281..9748)
                     /gene="smpB"
                     /locus_tag="P5316_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003954767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRR85592.1"
                     /translation="MASQTPEQRRIVNKKARHQYHIIDTVEAGIVLTGCEVRSIREGR
                     AQITDGFVRINGYEATLYGCQIDRYEHGFQASYDPKRKRRLLLHRREIRRLSSQLAQK
                     GCTLIPISIYFNSRGLAKVELGLAMGKAQYDKRQDLKKRQHQRDIDRATGRRR"
     gene            9904..10533
                     /locus_tag="P5316_11345"
     CDS             9904..10533
                     /locus_tag="P5316_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR85593.1"
                     /translation="MILRQAENGVQRLTRRMERRLVAQARGGDEDAARRLVDSHKDRL
                     FAFVWRILRDHEDAEEICQEAFLRAFSSLDSFSPEYRFSTWLFTIAYRLCLNQIRRKK
                     ALTGEVDFSNLSASEDDVCMQLAESEEARRLKDKIWEAVDELSVQQRAAILLFYREEM
                     SCDQIAQTLGIPMATAKSHLHRARGRLRDALSGLVKDWTQVQALREAAS"
     gene            10530..11288
                     /locus_tag="P5316_11350"
     CDS             10530..11288
                     /locus_tag="P5316_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRR85594.1"
                     /translation="MITCQHARQLFDRYLDGELSPSLQAELHAHQLSCSACQTELAML
                     EACGDVIMLDRRREPKLSDSFTDRVLLARRAQLASQPRRRHWARTLVVVASPLAAAAS
                     IALCLTLMWPDAPQRQTKTGVAVRVEVPPGSTTAVYGGSSAQGNPDPNLPATQMPSSA
                     LMDSILTTVVERTSRTVERAKNNFSHLELLFQRALAETNAQVAAQDAGSPRRASSGQR
                     SADQPGAEQELLNSPGTGFENSGDSGNDQLLEPL"
     gene            11416..13560
                     /locus_tag="P5316_11355"
     CDS             11416..13560
                     /locus_tag="P5316_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85595.1"
                     /translation="MAAACWLSLGLGMAGTMAADATSPALLARSVPADARLYLEFHAL
                     GSFAGTPFDLMMSRMVGDLVTSGSGAATQPDTTPPAAQPTAWRAWFAETVGLQDPKAA
                     DLLFSGPIAFAAEGWSGLGDAVLLAEPEDVSGLETVMKPHLASSSPKVRVYRLSQEHE
                     LACDGRVAVLGRSGESINLYRRTVALWESDGGATLGDMAEFRERMAGLPTGAQIIFYA
                     GSTARAAEARFILGQWWPADWVQLRSVAIGVGVSPAGLMFDVDAHADPDSQPQPAPPL
                     AADALSRLPASVVAAWTRPIDFVERFRRLQVDDPTGIIEVLLADMPGRSIEWQLLDHL
                     GDSAVFVLARRPMFAGTASSFQPDVATARGTTTMPAPASSAPSASLEAAPASAASGPA
                     AREPVLILPVLAMLVETDDPHAVEAALERITLNLQYLVASRSPGDAGGVVSRVPERGG
                     GQMVSVSLRRLFESRTKCPFLLNLELSWIVWDRWLIIGTNTDVVHEIVAARRGEGQLL
                     PPGLMPHLVAEVSQSGTVGQMLLLGQPRDLAEMLDSWVAYVSAYHPQMLLAEWWRDLE
                     RRQRSMGVQLGVVPTARAVRSAVEVGQTLPGYPAFGVLQPGDLILRVDGHVLSEDDPS
                     RSLRQLLSVAGESGKVTLRILRGGREKDVAITMPAVTESARTVQPVTLLKQIAGFLRP
                     FPTAGYAVWRPGPERLKAHLELRLAHSTRPSE"
     gene            13811..14284
                     /locus_tag="P5316_11360"
     CDS             13811..14284
                     /locus_tag="P5316_11360"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR85596.1"
                     /translation="MIVTEQQFGLLGLTNEQLNALLDDMDKACKETKASEHRRSIRKP
                     FRSVNVKVSILDKASREAVSFKVPTRNISLHGLAFLHHRMLQVGQMLRIQIPFRKGYT
                     VELLARVARCRHISGMIHEIGAEFKGRLSGHVISNEDEAAQTLESAAPEPPLADV"
     gene            complement(14307..14660)
                     /locus_tag="P5316_11365"
     CDS             complement(14307..14660)
                     /locus_tag="P5316_11365"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRR85597.1"
                     /translation="MPDYVFVIDARDVPPGRAVPAMVNDRWFAVCNDNGIFHVTAFNC
                     PHENGPLGKGYVRDGALICPVHHWPWDLKTGLTDPRMPHLRLKIYPCELRDGRVYADI
                     THPIPIGNRNLSGSP"
     gene            complement(14719..15195)
                     /locus_tag="P5316_11370"
     CDS             complement(14719..15195)
                     /locus_tag="P5316_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85598.1"
                     /translation="MTRSKDYPIVRFLIGSLYGISILSLLFGLASGAYLWVHAEALRS
                     SVIAGGPLAGTLSRFSSSQQLYQAAAILAGAGMVGFLVFGALGQILAIQRDRAINASL
                     QTQLLEDILELNEEITLASRQSRVEICEGCGRLGSLHRIESGQWVCRECRLQLRTA"
     gene            15709..16824
                     /gene="lpxK"
                     /locus_tag="P5316_11375"
     CDS             15709..16824
                     /gene="lpxK"
                     /locus_tag="P5316_11375"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRR85599.1"
                     /translation="MAESTTYMEIVSGQRRGLLASLARGSLWIISKGYGSLLVVRNAW
                     YDSVALPKWLDVPVISVGNLTVGGTGKTPMCGWLCRQLLGRGLKPAVLSRGYKASEEG
                     LADEMLMLSRQYPQAVLVAHPDRYGAGCVAVEHYGVQAVILDDGFQHRRIGRDLDLLL
                     VDATRPFGFGHLLPRGLLREPLKGMARAGAVVLTRCDLAGSVQVAELEKNVRRLHPEA
                     PVVRAVHRPRGFTGLDGAPVPMPPADRIGCLAGIARPDAFERTLADMGIRVAQIQWWP
                     DHHCYTAADAEMIRRWAEQNKLDALITTEKDAVKLEALKVDWPLPVVALHVEIDMLDD
                     GEAILSGLIDEVLKEHSEPETLAAKENGDLDEEHSHR"
     gene            16802..17641
                     /locus_tag="P5316_11380"
     CDS             16802..17641
                     /locus_tag="P5316_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRR85600.1"
                     /translation="MKSIHIVEKTLPMAWEKAVIACWEQGERFPTQYDKPEDPNSRDV
                     TAMIHVTEPMSEPRLHRAFPGGLDDLEKYRDEVLLGVHDHWIAPQEGKWEYTYHERLF
                     EYDPPWAGGGKIDQIATCVRLLKDCWYTRRAQAVTWKAWEDLGISDPACLQRMWFRIQ
                     NGRLNMNVHMRSNDAYKAAFMNMFAFVELQAQMARDIGVVVGEYIHIADSFHIYGSYF
                     EEFQGFLKTVENRAVGDRVWDTGFAREMFIEGCDVLLAEQDMPQDKKELVRKRRAELT
                     ATG"
     gene            17685..>18148
                     /locus_tag="P5316_11385"
     CDS             17685..>18148
                     /locus_tag="P5316_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HRR85601.1"
                     /translation="MRIAIIGSGSVGCYYGAKLARCGHDVRFLMRRDLEAVRRSGLTI
                     KSCEGDFRIKAAAFGDPAEIGPVDLVICALKATAIDDAERLIRPCLGPETPILALMNG
                     LGIEERFVQWFGPERVFGGMAFVCINRGEPGVVHHLAYGRVATRSARAARPGL"
BASE COUNT         3279 a         5282 c         5797 g         3790 t
ORIGIN      
        1 acctgggtgc tttagcgcag gaagtaaccg gcaaccccga ctggatgagc agcgtccctt
       61 cctggcagac tcccatattc aaggactacg attccgacta ccaattcatc tttgtgttgg
      121 cggtctttta tctattccgg aacattctgg gcggattggg cgcgggcgcc gatcctcgtt
      181 acttcggggc ccgcagcgag cgggagtgcg gcctgctgac gttcttctgg acctggctga
      241 tggcgtttcg ctggccgctg atgatgggtt tcgcggccat ggggttgttc ctggtcaagg
      301 gcatgtttcc caatcaggag gttctcgccc aggcatccca gatggtcaag cgctatgttg
      361 ctgagcaggc aggcgcccga ggccccgatg cgttggccga caaggatctg atgaagagac
      421 tgctgccgag ggagaggtgg gaagacaagc tctccgacat cagccggcac cccgagaaat
      481 atcccccgtt ggctgattcc ctgagagggg tgctcggtga cggctggcag agcaagttgg
      541 acatggtcag ctacgaagga cagatcaacc cggaacggat tctgcccgcg gtcattctgc
      601 aatatcttcc gatgggcctg cgcgggctga tgctggtttc tctggtggcg gcggcgatgt
      661 ccactttctc tccgaccgtc aacatctcga ccgccttgtt cacccgcgac atctggcagg
      721 ccttcgttcg accgaaggcg tccaacccgg aactcatcgc cgtgagctgg atgttcgggg
      781 ttcttctgac cgcgggcggc tttgcgatgg cctacacgag caagaacatc aacgacattt
      841 gggactggat catcatgggc gtgacggccg gacttgccat tcccaacctg ttgcgactct
      901 attggtggag gttcaacgcc gggggagtcg tggtggggac cttcattgcg gtcgtcgggg
      961 cggtggcgca gcggatcatc tggccggaca tgggtccgta cctgaagttc ggtctgaaca
     1021 ccagcgtctc ctttgccggc tgcatcatcg gcactttcct gaccaagccg accgatccga
     1081 gagtggtgga acacttttac cgcacgacgc gtccgttcgg gttctggggg ccgttgaaaa
     1141 agctcttgcc gcccgacatg cgaactgaga tggatcgcga gaaccgccgc gatattctgt
     1201 cattgccctt tgccctcgga tggcaggtca cgttgttcct gctgccgatg cagcttatca
     1261 tcggggcgtt tagagacttc gcgatcacgc tggtggtttt cctggtgtgt ctgggctggc
     1321 tgctggttgt ctggtatcgc aacctgccgg ccgacgactc gcccctcacg aaggccaatc
     1381 cggtattccc ccctgcttcc tgagcgcaga ggccggttgg gcgatgcgag cttctcacaa
     1441 ggggttggga tcgcacgggt ggaagccatt cacgtttgcc ggcagcgggc gggggttccg
     1501 agccggccgg cgtgacgcag gttcccgtcg cccgacaagg ccctatgcgt cggagcttgt
     1561 ttgggcaagg caacggctct tcgccagatt tctccggatg gtttcgcgca acgctgcgat
     1621 cttgatcggc ttggtggcat aatcatcgca cccggcctcg atgcatttgt tcttgtcctc
     1681 ggccatggca tgcgcggtga gggcgatgat aacaccggtg tagcctttcg cccgcagcat
     1741 tcgagtcgcc ttcaaaccgt ccatgacggg catctggccg tccatcagaa cgacatcgaa
     1801 cgggtgtacc gccgcctggg cagccatgac cttctcgact gcctcccggc cgttgtccgc
     1861 cgcggttact ctggccccgc attttgtcag cagtcggacg acgagcattt gcgtgtcgcg
     1921 tgtgtcctcc gccagcaaga tgttgcaatt cagcaaatcg cccgcgtttg ggccggcggc
     1981 aggttccgcg gcgagttgcg ccgccgtaga ggcttctctg ctcggctccg gctcatcgac
     2041 cggatcgaag ggcagcgtca gacggaaggt gctgcccttt cccggcgcgc tctcaagcag
     2101 cgtgagggtg ccgccgagca tctccgcgag ccgcttgctg atggccagac caaggcccgt
     2161 accgccgaaa cggcgtgcgc tggagctgtc cacctgcgtg aaaggctcaa acaacctgct
     2221 ttgctgctcg acgccgatgc cgattccggt gtccgacacg tccacgcaga tgcatgcatt
     2281 cccgccgcta tcctccatga acgaagcggt cagagacacg attccggagt cagtgaattt
     2341 gacggcattt ccgagcagat tgatgagaat ctgacgcaac cgcacgacat cggtacggat
     2401 cacctccggg acggtttcct tgcactcgac cgacaacggc aagccttttt ccccggccct
     2461 gacgcgcatg atcgattcga cgtcctcgat tacccgtctc aacgaggccg gtgctcgaaa
     2521 gacctcgatc ttgccggcct cgatcttcga gagatccaga atatcgttga tgatcaacag
     2581 caggtgctcg gcgtttctgg cgatgatctc ggtatactcc cgcatgtatg agtcgccgaa
     2641 ttcgcatgag tatgggcaac ccttgctcaa ctcctccaca tagcccagaa tggccgtcat
     2701 cggcgtgcgg atctcgtgac tcatgttggc caggaactcg cttttggcct gattggccgt
     2761 ctcggcggct tccttggcat gctgcaggtc cgcttccacg atctttcgtt cggtgatgtc
     2821 gcgagcgaag gcgaaaatgt atgcctgccc gtcgaattcg aggtagttgg cgctgatttc
     2881 caccgggaac accgacccgt cccggtgttt gtgacgggac tcgaattcca ggaatccaca
     2941 actcctcatg tgttgccaat gagcacgcca tctgtcgggg ggaaaatcag gatcgatgtc
     3001 gtgaacggtc atggactgaa gttcctcgcg ggagtatccc aaccgccgac aggtggcctc
     3061 gttcacatag aagagccgtg cgtcctcgct gatccagaag atgctgtcgc gggcatgctc
     3121 gaccgagaag ctgttgagtt gaagggcttt ctcggcctgt ttgcgctcgg cgatctcgtg
     3181 cgccaattcg acgttggcct tacgcagctc ggcggtgcgc tctgcaaccc gctgttcgag
     3241 caactcgcga tcggaacgca gcctctcgga cagtcgattg aagctttcgg ccagattccc
     3301 gatctcgtcc tgctgcgtga cctgcagggc ttggacctcg cggctctcgg gatccgtcgc
     3361 caggagctgg aagtgttgag cgatagccgt gagccgcttc gacaccgttt tgcggaacac
     3421 gagtgcgatc atgccgagca tgccggccag gccgaccagc atggtcatcg agtcgcggac
     3481 catatgggct ttcagcgcag actgggcgga gtctacgggg atggccacgg tgtcgagggc
     3541 gatcaccttg ccgagcgggc gatggaagcc ggccacattg ccgtatcgcc ggatcagtga
     3601 agccggcgca tcttccggct ccccgtggca gcgaaggcag ctttcctgca ttcgcctggc
     3661 gctgaagtgg gccagatacc gccgcccgtc cagtcggatt tcgccgctcc agttgttcac
     3721 ctgaggattg gcgtcgaagt actcgatcat cttcaactca tccgggccgg ccttgttcac
     3781 cgggttgcgg gggttgtccg acgagaaccg gatgatgtag tgggggaatc ggccgcggac
     3841 tcgctcgaaa atgctccggg cgacgaacga ggtcgacatg gtctccggat ggaactcgtc
     3901 ctttgcgact cgtttctcca tctcggggcg tatctgtgcc ccgacgtagt cgcggatggc
     3961 gagatcgaat tcgagtgcga gttcggcctg ctgcttgagc aggagggctg cttgatcgag
     4021 gcggtcggcg taggcgcgca ggagcagaaa cgtcgtgaac agcacgacga agacgccggt
     4081 tggcaacaga aacctgttcg cgatgctctt catccacggc ctcggtaacg gcgcaggctt
     4141 tgtgcgtagc ggcagggctt cccgtcacgc gccgcgccgt tcgctcattg gttagatcga
     4201 agcgatcaag cggtgccata agacgcggtg acgtccgggt ttcggattcg ggtgtatgcc
     4261 cggcgattgg aaccaccgct cggggcttat cggccccctt gggccttttt ccggtcctcc
     4321 aatgccgtag gtgtcccatt tccgaccacg agttcacaaa gcgccgctgg aaagaagggg
     4381 gtgtcagatg atgctccggc tgctcacggg aaccagggac ggcaacatga ctggttgcac
     4441 ggtgagcaga gtggtcgatt gacacccctt gcccgtttgg tactctgagg gacggcgagt
     4501 tgacgacggg caactggcca tcgcatggag aggtgccgga gtggctgatc gggctggttt
     4561 cgaaaaccag tgtgccagca atggtaccgg gggttcgaat ccccccctct ccgtttttca
     4621 tgccttcggc aatcgtaagt gtttgccaaa taagcactag cggtttcgga gctctcccgc
     4681 ttgtgcatgt tcttgcgcac aatcatgcgg gttgattctt ggtcatggtc tttttcatga
     4741 gggtttggcg acgccgagtc ggccgcttcg ccgtttgcga gcatggcggt cggttccgga
     4801 tctactgccg gaactatcat ccggtccgtc ggccggcgag cacgaatcgc gacgacgcgt
     4861 tggtcatcgc ggcgaggatc actctgccat ctctcgcagt tcgatgtagt cctggtcgtc
     4921 ggtgccgagg cccaactctg cggcggcgcg aatatgcggt acgtggccgg agccggtgcc
     4981 gatggacgcc aagccgagac ggccgcgtcg gtcgttgatg atctcgagac cgacggcgtc
     5041 ggctgcgaca ggatcttgcg agacgatgag tccggcgtga tcccacgttc cgtccggtcg
     5101 aacgtttggg ccacccttgt ataacgcgcg aatggcgttg acgatgttaa ggcgcagctt
     5161 gttgcggatc tgtgggaggg ccacgatgtt gcccacataa ggagcgcagc cgtggtcatg
     5221 atagcggccg ggatgccgta tcaacgcgtg cgagaggttc ttcaggcagc ccgtgatgcc
     5281 ggcgatgttg tgcgtcttga ggaatggtac gttgatgatg gcagtgacct cctgcaagac
     5341 ggccgccaac tcctccgacc cgccgtcaaa ggctacctcg ccgccactcc agccggtgac
     5401 ctcgggccgt gtgcccaaac tagcgccgag cggtgccggg acttcgatga gcatgatccg
     5461 agccggtgaa aaacccgcgt cgccgaggga agcgaccagt tgggccgcga tcgcctccgt
     5521 ggtgttgaat tcctttgccc cgacctggtt gaacttgatt ccgatgacgt cctccggttt
     5581 gaacagtctg ttccatgcat cagcaggccg cagcgtgtcg gtcagcacct tgaggctctc
     5641 ttcgagcatc tcacgaacaa gcggaggatg aatggtttgg cggtcgacga cctcggatct
     5701 gcgaatgtga acgaccgtgg atttcaggag gctgtcaatg gggcccgtat ccggcctggt
     5761 ttgcagaacc ggtagagtgg tcgcaggatc ctgagcggct tgcaggctgc ggccggccag
     5821 cagtcctcct gccatcgtcg ccaaacctgc ttggccgacc cgtttcagga atgtgcggcg
     5881 gttctcagat cgcatcccga cacccggcgc accgcctggt ggcccgtgac actgttgttt
     5941 gttgaccaga acgctgtcgc gcctatagtt atcgacgatc gacaggggtg ccctgaggtc
     6001 tgcctttttg tcccgcgaca cccgggtctg cgcccctaac ctattgtcga ccataaggtt
     6061 atttgactgg agggacgcgt gaaccggatg gttgcgtgag cggcgcatga actctccaat
     6121 aatccagagt gagtcggaac aggcaaaggc gatccaagcc cgtcttcggg aggccaatca
     6181 gacccatctg ctggcccatt ggcatcagtt ggacgatcag caaagagagc tcctgctgga
     6241 agacattaac cggatcaacc ttgccggctt gtcgggtctg atcaagagtc atgtgatggc
     6301 cgatcggcct ttcaccctgc ctcgcgacat ccgaccggtc gtctgctatc ctgcccggcc
     6361 gggaatcgac caagtgggct tgtatgctga cgcggtaaag cggggcgtct ccctgatacg
     6421 ccgtaacaag gtggccgctc tgacggtggc cggaggccaa ggcacaaggc tgggttttga
     6481 cggtcctaag ggggctttcc ccatttctcc cgtcaagagc aagccccttt ttcagctctt
     6541 tgccgaattc atccgcggta cgaatcgtcg ttacggggcc gacttgcact ggtacatcat
     6601 gaccagtcct caaaaccatg ccgacacggt cgcgttcttc gaagccaatc acttttttgg
     6661 cctcaggccg cagcgtgttc ggtttttccg gcaaggcatc atgccggctt tctcaatgga
     6721 cgggaagatt ctgcttgacc agaaacaccg gattgccttt tcccctgacg gacacggagg
     6781 cagcttgctg gccttgcggc gatcgggaat gctcgcggag atggccgagg ccggcattga
     6841 gcatttgagc tatttccagg tggacaaccc ccttgttaag gccatcgatc cgctgttcat
     6901 cgggctgcac gatatgaccg gctcggaaat gtcaagcaaa acaatcccca aggtcgacga
     6961 tctcgaacga gtggggaatt tcgtgatcgg cgacggtaag gccatggtga tcgagtactc
     7021 cgaccttccc gacgagctgg cgcatgcccg cgatgccaac gggaaccggc tttttgatgc
     7081 cgggagcatc gccattcatc tgctgagccg ccgttttgtg gaaagactga cggccgatga
     7141 ggcacatttt gggctgcctt ggcatcgcgc ggtgaagaag gtgccttacg tcgatgagag
     7201 gggccggcga atcgagcctg agaagcccaa tgctgtcaag ctggagagtt ttattttcga
     7261 cgccattccg ttagctggga aacctctgat cctgcaaacc atcagagaag aagagttcag
     7321 cccggtcaag aacgtaaccg gcgtcgacag cgccgaaagc tcgcgccggg acttgaatcg
     7381 gcgcgccgcc cgctggctgg agtctgccgg ttatgccgtt ccctggcgcg agggggaacc
     7441 ggatggtctg ttcgagatca gtcctcttct ggctctggat gcggaccatt tgcgcgagca
     7501 aatgtcaaca cctcccagga tcaccccggg gggtgcccac tactgggagt agcgccgaat
     7561 ggggtcttgg cacgtcgcgt agggtgccgg attcgcgatt ggacggcttc ggatgcaaaa
     7621 acgcattgac gggcaccgga aggggcacga gaatagagtt gcaaatcgtt gaccgctcgc
     7681 tagactaggg ccgctgggat tgcctgcgag tggaatgtgg agtggatgac acatcggcaa
     7741 tcgttcacga agggattttg atcatgacag actcatccaa gggtacattg ctggtcgttg
     7801 atgatgagcc actgaaaagg gcgacgcttc agatcgaact ggcgggggca ggctataccg
     7861 tccttgaggc cgccgacgga gcggctgcgg tgaagttgat cgagacgcgc cccgtggatg
     7921 tcgtcatcac cgacgtgcgt atgccggaga tggacggcat tcaactgctt gaggcggtca
     7981 agtcgcgctc gccaagaacc cacgttattc tgatgaccgc ctatggcacg gtggattcgg
     8041 cggtcgaggc catcaagcgc ggggcctacg attacatcaa caagcctttc gcgacggagg
     8101 cgctcgttga gaaactggat cggttgctgg cctgccggtc ggcggaaggc aacggtcacg
     8161 gcggtccgga aacactgggc caactggtcg gccagagcta tgccagtcgg cggctcttcg
     8221 agcagatacg ggccgtggct gacagcgacc gaaccgtcct ggtcgagggc gaggccggta
     8281 cctgccggca gcgggttgcc gccacgatcc accagctctg caagcggtcg gccggtccgc
     8341 tggtaagatt caactgcacc gcgtgtgtcg ccgaggtcct ggaaagcgaa ttgttcggaa
     8401 ccggcgacga gggggccgct caacgtatcg ggcggttgga ggaggcggcg ggaggaacgt
     8461 tgttcctcga cgagatcgag gttcttccga tgccgttgca ggcccgactc ctgcaggcca
     8521 tggatgaggg ctttttcaat cgcgtgggcg gcggacgcgt gcggttggac gccagactgg
     8581 tgttcgggac gcaggagaat ctccggcgca aggtcgaagc cggccaattc cgcgaggatc
     8641 tttactatcg tcttgatgcc gtgcacctcg tgatcccgcc gctgcgagat cgccgcgagg
     8701 acattcccgc gctggcggcc cattatctgc gccactccgc caaggcggcc gagggcaagc
     8761 cggtgccgac ccgcatcagc acccacgcca tggacgtgtt gatgtcctac cactggccgg
     8821 gcaacatcag cgaactggaa aacgtgcttg agcgagcggt tgccttcgcc accgggccgg
     8881 agattcaacc gcgtgatatc ctggtgccct cggcgggcca ggcgagccag gaggttgtgg
     8941 ctcagacgac catgcatccg ggtttgacgg aaaccatcgc cggggtcgaa caatcgctga
     9001 ttgacgcggc tttgcgaaag gccgccggca atcaggccaa ggctgcccag tttcttggca
     9061 ttccacgaac gacgctcagg gacaagatgg ccaagtacgg tatggtgggc aagcctgcca
     9121 aacgcgaatt ggcctagctg gtcgtgcctg ccgtttaagg gttcttcggc ggccggtgga
     9181 aacgccggcc gtcgttcttt gtgacgccca caactccctg ccacatacag gctatcaaga
     9241 cccggcggaa tgggagccgg tcgatcaggt cctcaccccg tcatcggcgg cggccggtag
     9301 cccgatcgat atcccgctgg tgctggcgtt tcttgagatc ttggcgtttg tcgtattgtg
     9361 ctttgcccat ggccaagccc agttcaacct tcgccagacc tcgtgaattg aaatagatgc
     9421 ttatcggtat cagggtgcag cccttctggg ccaactggga ggacagtcgt cggatctctc
     9481 ggcgatgcaa gagcaaccgg cgcttgcgtt ttgggtcgta gcttgcttga aagccgtgtt
     9541 cgtagcgatc gatctggcat ccataaagtg ttgcctcgta gccatttatg cgcacgaagc
     9601 cgtctgtgat ttgtgctcgt ccctccctga tgctcctgac ctcgcagcca gtcaggacaa
     9661 tgccggcctc gacggtgtcg ataatgtgat actgatgcct ggccttcttg ttgacgatcc
     9721 gtcgctgctc gggcgtctgg ctggccataa ctgttgattt cccagaagat taaaccgaca
     9781 atatagaagc cgggccgggc ttgtcgggcc aggctatagg gcgtattcta gcgggtgctc
     9841 tagaagctgc caatcaagtg caactatggc ggcgcgcgca tgtataatta accgagaaga
     9901 gacatgatcc tgcggcaggc cgagaacggt gttcagaggc tgaccaggcg gatggagaga
     9961 aggctggtag cccaagcccg aggcggggac gaggatgccg cgcgccgctt ggtcgattcg
    10021 cacaaagacc ggctttttgc ctttgtgtgg cgaatcctgc gggatcatga ggacgccgaa
    10081 gagatctgcc aggaggcgtt ccttcgggcg ttcagttcgc tcgactcgtt ttcgcctgag
    10141 tatcggttca gcacttggtt gttcaccatt gcttatcggc tgtgcttgaa ccagatccgg
    10201 cgcaagaagg ccctgacggg cgaggtcgat ttctcgaatc tttcggcatc cgaagatgac
    10261 gtttgcatgc agttggccga aagcgaggaa gccaggcgat tgaaggacaa gatttgggag
    10321 gccgttgacg agctttccgt gcagcagcga gcggccatcc tgttgtttta tcgggaagag
    10381 atgagttgtg accagatcgc gcaaacgctc gggataccca tggccacggc caagagtcat
    10441 ctgcaccgtg cccggggtcg cctcagggac gcattgtccg gtctggtaaa ggattggaca
    10501 caggttcagg ctttaagaga agcggcgtca tgatcacttg tcaacatgca cgacagttgt
    10561 tcgaccgcta ccttgatggg gagttgtccc cgtcgttgca ggcggaattg catgctcacc
    10621 agttgagctg ttcagcctgt cagaccgagc tggcgatgct cgaggcgtgc ggcgacgtga
    10681 ttatgttgga tcggcgccgt gaaccgaagc tcagcgactc cttcaccgat cgcgttctct
    10741 tggcccgtcg tgctcaattg gcctctcaac cccgccgccg gcactgggct cgtacccttg
    10801 tggtggtggc ttccccgctg gccgccgccg ccagcatcgc cttgtgcctc acgctgatgt
    10861 ggccggatgc cccgcagcga caaaccaaga cgggtgtcgc ggtccgggtg gaagtgcccc
    10921 ctggcagcac gacagcggtt tatggcgggt cctccgcaca agggaatccg gatccgaatc
    10981 tgcccgccac ccagatgcct tcgtctgctc tgatggactc gatcctgacg accgtcgtcg
    11041 aacggaccag ccgtaccgtt gagagagcaa agaacaactt ctcccatctc gagctgctgt
    11101 tccagcgagc cttggcggaa acgaacgctc aggttgcggc tcaggacgcc ggttcgccgc
    11161 gcagggcatc ctcaggtcag cggtcggcgg atcagccggg tgccgaacag gagttgctga
    11221 attcaccggg gacgggtttc gagaacagtg gcgactccgg caacgatcaa ctgctcgaac
    11281 cgctgtgatc caatcgtatc cggccacggt tgttgccggg tcatccaatg gtatagtacg
    11341 tgtgttgttt aatggtaaaa cggcgggggc cgtcaggggg tttgttttgc catgcccggc
    11401 gggtctggat tgcgtatggc ggcggcatgt tggctgagct tggggcttgg catggccggc
    11461 accatggctg cggatgccac ctcgccggct ttgctcgccc ggtcggttcc cgcggatgcc
    11521 cgcttgtatc tcgaattcca cgccttgggc tccttcgcag gcaccccgtt tgatctgatg
    11581 atgtctcgaa tggttgggga cctggtcacg tcagggtctg gcgcagcaac gcaaccagac
    11641 acgacaccgc cggctgctca gcccaccgct tggcgggcat ggtttgccga gaccgtgggc
    11701 ttgcaggatc ccaaggccgc cgatctgctt ttctccggtc cgatcgcctt cgcggccgag
    11761 ggttggagtg gtttgggtga tgccgtactg cttgccgagc cggaggacgt ctctgggctc
    11821 gagacggtca tgaaacctca cctggcctcg tcttctccga aggtgcgtgt gtacaggctt
    11881 tcgcaggagc acgaactggc ctgcgacgga cgagtcgccg tgttgggccg gtcgggcgag
    11941 tccatcaacc tgtatcggcg cacggtagcc ctttgggaaa gcgacggcgg cgcgactctg
    12001 ggcgacatgg ccgagtttcg cgagcgaatg gccggcctgc caacgggagc gcagatcatc
    12061 ttttacgccg gttcgaccgc gcgggcggcc gaagcaaggt tcattctcgg ccagtggtgg
    12121 cctgcggatt gggtgcagtt gcggtccgtc gcgatcggag tcggcgtcag cccggcgggt
    12181 ttgatgttcg atgtcgacgc ccacgctgat ccggattccc aaccgcagcc ggccccgccg
    12241 ttggccgccg acgcattgtc gaggttgccg gcttcggttg ttgccgcttg gacccggccg
    12301 atcgactttg tcgaacgttt caggcgtctt caggtggatg acccgactgg aatcatcgag
    12361 gtactgttgg cggatatgcc cggcaggtcc atcgaatggc aactactcga tcacttggga
    12421 gacagcgcgg tatttgtgtt ggcccgacgc ccgatgttcg ccgggacggc aagcagtttc
    12481 cagcccgatg ttgcgactgc tcgcggcacg acgaccatgc ctgccccggc gtcttcggcg
    12541 ccttccgcct cgcttgaggc ggcacccgcg agcgcggcaa gcggccccgc ggcgcgcgag
    12601 ccggtcttga ttcttcccgt attggccatg ctcgtggaaa ccgatgatcc acatgcggtt
    12661 gaagcagctc tcgagcggat cacgctcaac ctccagtatc tggttgcaag ccgttcgccc
    12721 ggtgacgccg gcggagtggt tagcagggtt cccgaacgcg gcggcggcca gatggtctcc
    12781 gtgtcgctca ggcgcctttt tgagtcgcgc accaaatgcc ccttcctcct taatctcgaa
    12841 ctgagttgga tcgtctggga tcgctggctg attatcggca cgaacacaga tgtcgtacat
    12901 gaaattgtcg ctgccaggcg cggggaggga cagttgctgc cccccgggct gatgccgcac
    12961 ctggttgccg aagtcagcca gtctggaacg gtcgggcaga tgttgcttct cggccagccc
    13021 cgtgacctcg ccgagatgct ggactcgtgg gtcgcttacg tctcggctta ccatccgcag
    13081 atgctgctgg cggagtggtg gcgggatctt gagcgtcgac agcggagcat gggcgttcaa
    13141 ttgggcgtcg tgcccaccgc ccgggcggtc cggtcggcgg tcgaggtagg ccagaccctc
    13201 cccggctatc ccgcgttcgg tgttcttcag cccggtgacc tgatactgcg cgtcgacgga
    13261 cacgtgctca gcgaggatga cccttctcga tcgctacggc aattactgtc ggtggccggg
    13321 gagagcggaa aggtaaccct ccgaatcctt cgcggcggtc gtgaaaagga cgtggcgata
    13381 accatgcctg cggtgacgga gtcggcacgg accgttcagc ccgtcacttt gctgaagcag
    13441 atcgccggtt ttctgcgtcc gtttccaacc gccggttatg ccgtctggcg cccgggcccc
    13501 gaaaggctca aagcccacct cgagctgcgc cttgcccatt caacacggcc gtcggaatga
    13561 ggtgccgcga gagatgccgt ctgcggcggt tgtccctgta tcaaccgtgc cggtattctc
    13621 ggtccttggt gatccgattc atcgcgccaa ggcagtcatt ccctctcccg gcagcggcgg
    13681 actcgtttga aagaggctct ccgccgtagg ctctgctaaa gccccttcct tgtttccccg
    13741 aaggacatta acgaagagcg ccacctctgt tccgcgtcgg cgttcaaccc tgttagctcg
    13801 ggtccggccc atgattgtga cggagcaaca attcggattg ctcggactga ccaacgagca
    13861 gctcaacgcc ttgttggatg acatggacaa ggcatgcaaa gagacaaagg cctcggagca
    13921 tcgccgctcg attcgcaagc cgttccggtc ggtcaatgtg aaggtgtcca ttctggataa
    13981 ggcctcgcgg gaggccgtca gcttcaaggt tcccacgcgc aacatctcgc ttcatggttt
    14041 agcttttctt catcaccgca tgctgcaggt cggtcagatg ctccgcattc agatcccctt
    14101 tcggaaaggc tataccgtcg agttgcttgc ccgggtcgcc agatgccgtc acatcagcgg
    14161 catgattcac gagatcggcg ctgagttcaa aggcaggctg agcggacacg tcatttcaaa
    14221 cgaggacgag gctgcgcaaa cgctcgaatc cgccgcgcct gagccgccgc tcgctgacgt
    14281 ctgatctgtc ggctcctgcg taagggttac ggtgatccgg agaggttccg attccctatc
    14341 gggatcgggt gggtgatgtc cgcgtatacc ctgccgtcgc gcaattcgca cgggtagatc
    14401 ttcagccgca ggtgtggcat ccggggatcc gtcaggccgg tcttgagatc ccacggccag
    14461 tggtgaacgg gacagatcag agccccatcg cgtacgtacc ccttgcccag cggtccgttc
    14521 tcatgcgggc agttgaacgc ggttacgtgg aagatgccgt tgtcattaca cacggcgaac
    14581 cagcgatcgt tcaccatcgc cggtactgcg cgtccgggcg ggacgtccct ggcatcgatg
    14641 acgaaaacgt aatccggcat gtgttcgctc gcgcgctcgg cacgtcgcgg gccgtgagga
    14701 tccggcgagg tgaggcggtc acgccgtccg aagctgcagc cggcattcgc gacataccca
    14761 ttgtccggat tcgatgcggt gaagggaccc gagacggccg caaccttcgc agatctccac
    14821 ccgagactgt cgcgaggcca gggtgatctc ctcgttaagt tcgaggatgt cttccagcag
    14881 ttgggtctgc agcgacgcgt tgatggcccg gtcacgttga atggccaaaa tctgtcccag
    14941 ggccccgaag accaggaagc ccaccatccc cgccccggcc aggatagccg ccgcctgata
    15001 aagttgctga gaacttgaga accggctgag agtgccggcc agcggaccac cggctatcac
    15061 gctgcttcgc agggcctccg catggaccca caagtaggcc ccggaggcca gcccgaagag
    15121 gagcgaaagg atcgagatcc cgtagaggga tccgatcaga aaacgaacga tcggatagtc
    15181 tttgcttcga gtcattgctt ccaccgcacg cattgtacgg atagggccgg gaaacaccaa
    15241 ccgcatatca agtcctcccg cggtcacagc gaaggccttg gcggactcgg atggttccat
    15301 caaaggccgt tgttcggcga cttggggacg caagtgtttg ttgcttatag acttacgctt
    15361 tccaaacgcg tttgcttggc gttcattcgc ccacagcgcc gtcaatctgg cgcgccgcgc
    15421 cttatgtctt atcgggctgg cacactgcgt atctccatat ttgcctcggg ttgtgtatct
    15481 ctatccggcg gttgttttca gattggccat cgcctcggcc gagcgatcga gctgccggcg
    15541 tcgctgatag gaggcggacc gttttgcccg gtccccgccc ggttgcaggt tctcagcagg
    15601 cgccaagacc gcccggggca tttgacgctc gcaggaggtg gtgagataaa ggtcgccggc
    15661 gcgtcggcgg ccatgtgagg tggtcggcgg ctgcctttga agagaaagat ggccgagtca
    15721 acgacttaca tggagatcgt cagcggtcag cggcgaggtc tgcttgcctc gttggcccgg
    15781 ggctcgctct ggattatttc gaaggggtat ggcagcctct tggtagtgag gaatgcctgg
    15841 tatgactcgg tagcgctgcc caaatggctt gatgtcccgg tgatttctgt cggcaacctg
    15901 acggtcggcg ggaccggtaa gacgccaatg tgcgggtggt tgtgtcgtca gcttcttggc
    15961 agggggctta agcccgccgt tctgagcagg ggctataagg cgtccgaaga aggcttggct
    16021 gatgaaatgc tgatgctctc aaggcaatac ccgcaggcgg ttctggttgc ccatcccgac
    16081 cggtacgggg ccggttgcgt cgcggtcgag cattatgggg tccaggctgt gattctggac
    16141 gatggcttcc agcatcgcag gatcggtcgc gatttggatc tcttgcttgt ggatgcaacc
    16201 cgtccgttcg gattcgggca tctcttgccc cgtggtctgc ttcgggagcc tctgaaaggg
    16261 atggcccggg ctggcgccgt ggttctgacc cgatgcgatc ttgccggatc agtccaggtg
    16321 gccgagttgg aaaagaacgt ccgacggctg catccggaag ccccggtggt acgcgcggtg
    16381 caccggccac gcgggttcac cgggctggat ggtgctcccg tacccatgcc cccggccgac
    16441 aggatcggtt gcctcgcagg catcgccaga ccggacgcat ttgagcgcac gttggctgac
    16501 atgggaattc gcgtggcgca aatccagtgg tggccggatc accactgcta caccgccgct
    16561 gatgcggaga tgattcgacg ctgggccgaa caaaacaagc tcgacgcgtt gatcaccacc
    16621 gagaaagacg cggtgaaact ggaggctttg aaggtcgact ggcccttgcc ggtcgtcgcc
    16681 cttcacgtcg agatcgacat gctggatgac ggcgaagcaa tactgtccgg actgatcgac
    16741 gaggtgctga aggagcactc cgagccggag accctggccg ccaaggaaaa cggagacctg
    16801 gatgaagagc attcacatcg ttgagaagac gttgccgatg gcatgggaga aagcggtcat
    16861 cgcgtgctgg gagcaggggg agcgttttcc cacccaatat gacaagcccg aggatcccaa
    16921 cagccgggac gtgacggcaa tgattcatgt caccgagccc atgtccgaac cccgcctgca
    16981 tcgggcgttt ccggggggcc tcgatgacct tgagaagtat cgcgatgagg ttctgctggg
    17041 cgttcacgat cactggattg ccccacaaga gggtaagtgg gaatacacgt atcacgaacg
    17101 tctgtttgag tatgacccac cgtgggccgg cggcgggaag atcgaccaga tcgccacgtg
    17161 cgtcaggctg cttaaggact gctggtacac gcggcgggct caggcggtga cctggaaagc
    17221 gtgggaggat ctcggcatca gcgaccccgc ctgcctgcag cggatgtggt ttcgcattca
    17281 gaacggtcgg ctcaacatga acgtgcacat gcggtcgaac gatgcttaca aggcggcttt
    17341 catgaacatg ttcgcgttcg tggagcttca ggcgcagatg gccagagaca tcggcgtcgt
    17401 agtcggcgag tacatccaca tcgccgattc gttccacatc tacggcagtt acttcgagga
    17461 gttccagggc tttctcaaga cggttgaaaa ccgcgcggtg ggggatcggg tctgggacac
    17521 cggatttgcc cgtgagatgt ttatcgaagg gtgcgacgtg ttgctggccg aacaggacat
    17581 gcctcaggac aaaaaggaac tggttcgcaa acggcgggcg gaattgacgg caacaggcta
    17641 ggaccttacg gccggaaagc cgcgccgctt gcacgcatgc ggtcatgaga atcgcgatca
    17701 ttggatccgg atcggtgggc tgttactacg gggccaagct ggcacggtgc ggtcatgacg
    17761 ttcgtttcct gatgcgccgc gatcttgagg ccgtcaggcg aagcggcctg accatcaaga
    17821 gctgcgaggg cgattttcga atcaaggcgg cggctttcgg cgatccggcc gaaatcgggc
    17881 cggtcgatct ggtgatttgc gcgctcaagg cgacggcgat cgacgacgcc gagcggctga
    17941 tccgaccctg tctcggtccg gagacgccga tccttgcact catgaacgga ttgggcatcg
    18001 aagaacgttt cgtgcagtgg ttcgggcccg agcgcgtgtt cggcggaatg gctttcgtat
    18061 gcatcaaccg gggcgagccg ggcgtggtgc atcacctggc ctatgggcgg gtggctacca
    18121 ggagcgcccg cgccgcccgc ccgggtct
//
LOCUS       DAOTPW010000110        18061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3125_length_18061_cov_5.758025, whole genome shotgun sequence.
ACCESSION   DAOTPW010000110 DAOTPW010000000
VERSION     DAOTPW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18061
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_18061_cov_5.758025"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5316_11390"
     CDS             <1..226
                     /locus_tag="P5316_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85602.1"
                     /translation="VVQYGFLGFSVVLLGVVIWLIRQLLEVLEANNRIIAANTEAIRD
                     LTNTTSDLLNLNRSLHDKIISRPCIAREEV"
     gene            228..656
                     /locus_tag="P5316_11395"
     CDS             228..656
                     /locus_tag="P5316_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85603.1"
                     /translation="MSVITQIADAVVTSLNGGSFSQSFTAQRRYQPSFALAEMETLRV
                     TVVPKSVSITNATRDSGFFDCAIDIGVQKKVNPEDQAEADALLGLVDELMTHLRGRRL
                     DDAPEAAWLSPTHDPIFAAEHLDSKHVLTSVLTVAYRVQR"
     gene            699..1127
                     /locus_tag="P5316_11400"
     CDS             699..1127
                     /locus_tag="P5316_11400"
                     /inference="COORDINATES: protein motif:HMM:NF017961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HRR85604.1"
                     /translation="MAIKLGMEAKLYYGPAGSTANTELNNVKDVTLNLETGEADVTTR
                     GNQGWRATVATLKSGTVEFEMIWDTGNAGFTAIKNAYFNNTSIALAILDGPNGEGLDA
                     DFSITNFSRNEPLEEAITVSVTAKPTYSTRAPTWKTAGGT"
     gene            1131..1592
                     /locus_tag="P5316_11405"
     CDS             1131..1592
                     /locus_tag="P5316_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85605.1"
                     /translation="MRTFTDNAGRTWTIVINVGAIKRVRGLLEVDLLEVVDGKLIERF
                     IRDPVLLCDVVYAACKPEADAKGVSDEEFGKAMAGDAIEHATKALLEELVGFSPSPRD
                     RANLQRVLEATWTAMDRARDVVEARLTNGELDRVVEQALATATNSSGGAPA"
     gene            1652..1852
                     /locus_tag="P5316_11410"
     CDS             1652..1852
                     /locus_tag="P5316_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85606.1"
                     /translation="MKDDWSRTSSLMALLANCHRDPKKTPPYRPSDFDPFVKRSQPPM
                     VGVEILKDVFVHGRIPKQEAHT"
     gene            1849..2238
                     /locus_tag="P5316_11415"
     CDS             1849..2238
                     /locus_tag="P5316_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85607.1"
                     /translation="MKNVAFAEFSIIGLIHAALVGCASLNPQPPIEAFANKLADEAII
                     PAVREGLAQGVEQLSIQAGAQGINPTYAVMFEGKWVVGIEGRATVGVEGVAGQIQVSS
                     TSNEETETSPHATGGGSATQPANREEP"
     gene            2383..2598
                     /locus_tag="P5316_11420"
     CDS             2383..2598
                     /locus_tag="P5316_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85608.1"
                     /translation="MLTWLDGKKTLIGSLLLSLLGAVWSLDVLIDGAANWLTEQQYVA
                     IGTTIAGLTGAAMRLAVGKVEKNGKTE"
     gene            2595..3035
                     /locus_tag="P5316_11425"
     CDS             2595..3035
                     /locus_tag="P5316_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85609.1"
                     /translation="MIDLRIKELFFDRPMVARAVNKAKRGVLSRAGAFIRQAARTSIR
                     KRQGTSSPGSPPYSHTGLLRRFILFGYDRGTDSVVVGPVRLNKPGEAPRVLELGGTTV
                     IEQRVRGRRVRARARIQRRPYMGPAMEKELPKFPALWRNSIRPG"
     gene            3039..5036
                     /locus_tag="P5316_11430"
     CDS             3039..5036
                     /locus_tag="P5316_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85610.1"
                     /translation="MASTQGIRAGRAFVELGVSDKLTAGLRRAQRQLRAFGEGLRSIG
                     TRMTAVAASVLAPLGATVKYFSSAGDTLNKMAARTGVSSEALSELGFAAEQSGADLET
                     LELGLRRMQKVIGDAADGSASAGDTLGKLGLTLAKLQGLAPEQQFALIADRLSQIQDP
                     TLRAASAMEVFGRSGTQLLPLMQDGARGIGQLREQARALGLTVSTGTAQDAAVLNDTL
                     NILWRVVKQGAFVVGSVLAPAVVELSNTITRTVVSVTNWISQNKAVVVTVAKVAATVA
                     AAGVAIIGLSWVVSGMAAALGGLAAVASGVGTVLGVVGSALTALLSPIGLVIAAVAAL
                     GAAVFIYSGAACKALEWLREQFGRLHAFVSRVVTGMSDALAAGDVALAARILWLALKL
                     AWQEGVAALNRTWLAVKRFMLTQAQQMWTGVLSGAEFVWHGLKVGWIEATAFISRTWK
                     RFTSFLEVTWATIKNVATKTLNHIKGLFDETFDVDAANLVADQALVAAEQRIEQDKDA
                     QLGQLQREGQQKREAEQQRHESRLRGIIEAEDEALAELDQATDSQLDQTRQQLDDARR
                     QLADALAEAKQKREQIEAEDKAPQAGPRGLLDGLADRLEGLGDVIANKITVTGTFNPV
                     AVQGLAAGNDAAQRTATASEQTAKHTKRLADAAVTGGLTFA"
     gene            5049..5858
                     /locus_tag="P5316_11435"
     CDS             5049..5858
                     /locus_tag="P5316_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85611.1"
                     /translation="MPITVEERFESRQVTMGTNPSAELRYNIRGTNDDVAARVALEAA
                     SPLVYDFYGNGLWVIPRESASVEPVANDIWEGIVRYGIVPQENESTFSFDTGGGTTHI
                     TQSLETVAAYGAVGTPPNFGGAIGVTDDNVEGVDITVPVYHFSETHFLPDIVVAPAYK
                     ATLFNLTGRVNGLPWKGFATGEVLFLGASGTKRGLQAWEITYRFAASPNKSNITVGSI
                     SGISKKGWEYLWIRYAETEDTAAKALVKRPLAAYVERVYEYGDFSLMGIGV"
     gene            5862..6668
                     /locus_tag="P5316_11440"
     CDS             5862..6668
                     /locus_tag="P5316_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85612.1"
                     /translation="MGELKKVQSGSPLVIPAQTFNAFIDAARDYQDRQLGQQRTALSS
                     SNDSGMVLLKNISGADRARFEILGINTPLIQPADNLDQFVNRPALSGVTPTAANHWGK
                     FGVLAEPIRNGALGWAWVSGVCPVKIDIANANHWHADIADGSAAYLKSSGGGAAQILW
                     KDSGTGVRWAVVRLGRWSPTVFPVNLTQTGGSQGTSTTAASWTYSVVDPVTNQTLATG
                     VNPTASPHKWKRPSAGWMIAATFGYAHYNAQGQLVLGWINEMVEQEACTS"
     gene            6681..7112
                     /locus_tag="P5316_11445"
     CDS             6681..7112
                     /locus_tag="P5316_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85613.1"
                     /translation="MATTGKARLFKAGKRTLRTNGKAQLFNAGGHCSECCTPYVLASF
                     TTNSSTPCWDLTPYQGPGKATPCAFWRLIETGSCYPYSYPWYGTGCVNHAGQLVGLPS
                     QFCSSYYYNGYMQLQIGCRSASGTQINWPGTCQTTTSVYSC"
     gene            7123..8325
                     /locus_tag="P5316_11450"
     CDS             7123..8325
                     /locus_tag="P5316_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85614.1"
                     /translation="MMRLDAIHQENVLTHMPFLTRLAASSDAEAIVDQGKLVLFYCRS
                     GPKDVDVSAGSRRWKLWIAVGDRPPRRLETGLPDDLTECSPTAWSDDTGWHVTFIAGG
                     ASESPLYRLYRMDGPDLDDLSKPVAIHATRAAFVYGDRLVHADPENLIYVRQPSGDFD
                     IELPGAFIYRVSYRADEPDTLLISGAWQTERDVFALEYDLRTGEQHLLECDGLPAYKC
                     TILGNKVLYAQRIGEHFEHRRINTSEALSRRRVNTIVRRQPGQVQPVVTQPTGGCGCR
                     GRSQTDATPLSASSEPPKAEASSAINSAADTAPVTRASCLECIEKHLGAALVLLTETR
                     EGYAHRLRAIGHLHEAEDESQEFPTLHAAIREARKAYQTAGTMPDWETLSQLAREPQR
                     EDGGHRER"
     gene            complement(8469..8957)
                     /locus_tag="P5316_11455"
     CDS             complement(8469..8957)
                     /locus_tag="P5316_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85615.1"
                     /translation="MLADYEVHPAGQCVPALGDADHLDAAVLKLRDDRAGFGEVAAEP
                     VELVDQHPFDLAGFGVGQQPEQGFTAGHRAAGAGPVNVFAVCLGLEVAAAGVELDFNR
                     RAAILGVGRVARVDCRLLHGPVSWALRMSTARRAMTRTNQLRALRICRRLGWKSSRGP
                     ST"
     gene            complement(9342..9983)
                     /locus_tag="P5316_11460"
     CDS             complement(9342..9983)
                     /locus_tag="P5316_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85616.1"
                     /translation="MRFGEGLPHRGGQPGGVIVGVAGQFRHGVLESLALGFQLPDLPR
                     YRPLGRGVDVRVHQEPGHRADPFVEFADLAANPLELVAVGLHQFPRFFNGVFNDRVGI
                     GEDLLVGRLDPVHQQVFPDVGHVVAGVAPVSLTAAASVVVVLALAVLAGPPITHAAAL
                     FAEDQPGERPVSRGKKLAGVSPRSRVQGQPILCFGEGSVVNDPQAFITLHDVL"
     gene            complement(10150..10222)
                     /locus_tag="P5316_11465"
     tRNA            complement(10150..10222)
                     /locus_tag="P5316_11465"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10187..10189),aa:Met,seq:cat)
     gene            complement(10473..11273)
                     /gene="nagB"
                     /locus_tag="P5316_11470"
     CDS             complement(10473..11273)
                     /gene="nagB"
                     /locus_tag="P5316_11470"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010378045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRR85617.1"
                     /translation="MEVIITKTADEASAFAADIIERKLMSKPAAVLGLATGSTPVGLY
                     KELAKRHREKGLDFSKVTTFNLDEYVGLPPSHPQSYRYFMEENLFKHINVPASAIHVP
                     YGHADSVLDFCAWYEDEIKRSGGIDIQILGIGGDGHIAFNEPGSSLGSRTRLKTLTEQ
                     TIRDNARFFSSMDEVPRFAITMGVGTILEARQIILLAFGASKADIVAEAVEGPVTSQV
                     TASALQLHPDVTVLLDQPAAAKLKRADYYRWVFEQKRRLVPKLIGHGG"
     gene            11589..12845
                     /locus_tag="P5316_11475"
     CDS             11589..12845
                     /locus_tag="P5316_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HRR85618.1"
                     /translation="MKLVVFEDSQFDRFLPLAWVRGVFELKTGATTLREKIERAAGTK
                     ATAVIARHYLAACVRRRSGSAAVNDLRGCKGDEVLFVNSRVRASQWTPPAKDGAQWHG
                     DQLAVWRTRDDVSGITDYDGLVAAASRTPKEEYTGKWFDYIWDVMLCNPEEIVADFKA
                     AGRNGVHGTMHESSVIYGPREQVFVGEGAEIHPFVCIDSRHGPVTLEAGVEVHPFTRI
                     EGPCYVGANSILLGAKVREGCSIGPMCRVGGEVEESIIHGYSNKYHDGFLGHAYVGEW
                     VNLGALTTNSDLKNDYSTVSMMMGGRTIDTGSTKVGSMIGDHVKTSIGTLLNTGTIVG
                     TMAILVATGAPLPKYIPPFAWFLNGFVGKGFGLNVLISTARTAMSRRKVELTADEEQL
                     LRHVFQLTDEERMSYVRKGRRAMLKK"
     gene            13118..13363
                     /locus_tag="P5316_11480"
     CDS             13118..13363
                     /locus_tag="P5316_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRR85619.1"
                     /translation="MADQDVLNRVIKVTARVLNLEPETIKPEHSFAFDLGAESVQSVE
                     LVAAFEEEFGIEMDEDAALAVATVGAAADFIGKHLKG"
     gene            13716..15857
                     /locus_tag="P5316_11485"
     CDS             13716..15857
                     /locus_tag="P5316_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRR85620.1"
                     /translation="MISSSGAGLDTIFRPRSIAVLGVTNTPGTVPHDIFVNLLNGRFN
                     GPVYPVAPRKRHIAGVKAYDYVLDIPDPIDMAILVFPGRVCELALEQCGQKGIKAAII
                     ISAGFREVGPAGAEREENIKAIARKYGMRLIGPNCLGAINTEPDVQMNASFARAMPAA
                     GSIAFISQSGALCTAVLDYAQGMNIGFSKFVSLGNKADVTEVDLLSYLADDPQTSVIL
                     MYLEEIRRGRELVQVAQHITAASKTPKPILAIKGGRTAQGAAAAQSHTGALASSDVVC
                     NAVFEQAGIIRSKSIEEMFNTARLLAYQPLPRGNRIAIVTNAGGPGVMATDAAIERGL
                     ELAKFAPETKAKLKKALPAAANMKNPIDVIGDARDDRYRAAMDAVYEDPNVDQILVIL
                     TPQSMTNITTIAQAVCEEQKRHADKQKPLMCSFMGAKDVAAGIAILQNHGIPHYVLPE
                     WATDAMEDAVRYRRWRDRKRTRIRIYEDVDRDTAASIIREASDGYLIEPLALKVLASY
                     GLPVVPSELTQTADEAVAAADRLGYPVVLRVVSTSIVHKFDVKGVMLNLRNADELRAA
                     CREMREAILEKYSAGDIAGFLVRKMIPAGKEVILGLSRDPVFGHVIMCGLGGIYVEAL
                     KDVTFRITPISEATATRMINSLRTTAVLKGLRGEAPSDIPAIEDCLKRLSQLATDFTD
                     IAELDINPLIVHPAGQGCHVADVRIRIQKLT"
     gene            16163..16690
                     /locus_tag="P5316_11490"
     CDS             16163..16690
                     /locus_tag="P5316_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85621.1"
                     /translation="MLAGCNGGPSRIPTEPQRRAMLSLMLPTHIKIQPFTRIASFDKD
                     NIPDGVIVVLRAIDRFGDPVKAAGPFYFELWSYQPASGEPKGQRLAFWDQTLDTADKV
                     SGHWTHAQMYEFKLGWPEGATSVRPGSKYVLTATYRTPWDETMTDEYVLDFSVPPGAI
                     QRAIERKPPQTAPGM"
     gene            complement(16846..17676)
                     /locus_tag="P5316_11495"
     CDS             complement(16846..17676)
                     /locus_tag="P5316_11495"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRR85622.1"
                     /translation="MARPRVVVVYNPDKPHVEATKTALLEDLRQRIDVVATGPIADTA
                     RLAESKPDRLIVLGGDGSILAVVRALKERQVPIIGVNFGKLGFLAEFTVEELDRNLDA
                     AVSASAAVSPRMMIAAEIADNARLVAQSVAVNDCVVQAGPPFRMVELSVSVNGDHLTN
                     ISGDGLVLATPSGSTAHNMSVGGPILQPETRAMVLSPISAHSLTHRPLVFAGDSVIEV
                     VAHSVNQGTTLVIDGQVSLPVHAGDRITVRRWPADFQLVRNPAYSKWHTLTTKLKWGR
                     "
BASE COUNT         3513 a         5596 c         5799 g         3153 t
ORIGIN      
        1 cgtggtgcag tacggcttcc tgggcttctc cgtggtcctg ctgggcgtgg tgatctggct
       61 cattcgccag ttgctggagg tgctggaggc gaacaaccgg attatcgcgg ccaacaccga
      121 ggcgattcgc gacctcacca acacaaccag cgatctgctg aacctgaatc gctcgctgca
      181 tgacaagatc atctcgcggc catgcattgc ccgggaggaa gtgtgagatg agcgtcatca
      241 cgcagatcgc cgatgccgtc gtgaccagcc tgaatggcgg ctcgttcagc cagtcgttta
      301 ccgcacagcg gcggtatcag ccgtcgttcg ccctggctga gatggagacg ctccgcgtca
      361 ccgtcgtgcc gaaatcggtg tcgatcacca atgccacccg cgacagcggc tttttcgatt
      421 gcgccatcga catcggcgtg cagaagaagg tcaacccaga agaccaggcc gaggcggatg
      481 cgctcttggg cctcgtcgat gaactcatga cccatctgcg gggccggcgg ttggacgatg
      541 cgcccgaagc ggcctggttg agcccgacgc acgatccgat cttcgcggcc gagcatctgg
      601 actccaagca tgtgctgacg agcgtactga ccgtggcgta ccgcgtgcag cggtagaaac
      661 acgccggacg ttggggccgg cgagatcagg agaaggttat ggctatcaaa ctgggcatgg
      721 aagccaagct ctactacggg ccggccggat cgacggccaa caccgaactg aacaacgtca
      781 aggatgtgac cttgaatctg gagacgggcg aggcggatgt caccacgcgc ggcaaccagg
      841 gctggcgggc gacggtggcc acgctcaaga gcggcaccgt tgagttcgag atgatctggg
      901 ataccggcaa cgcgggcttc accgcgatca agaacgccta cttcaacaac acgtcaatcg
      961 ccctggcgat tctcgatggc cccaacggcg aaggcctcga cgcggacttc tcgatcacca
     1021 acttcagccg caacgagccg ctggaagaag cgatcaccgt cagcgtgacc gccaagccga
     1081 cctattcgac ccgggcaccg acctggaaga ccgcaggagg aacgtaatcc atgcgaacct
     1141 ttaccgacaa tgccggccgg acttggacca tcgttatcaa cgtcggtgcg atcaagcgcg
     1201 tgcgcgggct gctggaggtg gacctgctcg aagtcgtcga tggcaagctg atcgagcggt
     1261 tcatccgcga tcctgtgctg ctgtgcgatg tggtctacgc ggcgtgcaag ccggaagcgg
     1321 atgccaaggg tgtcagcgac gaggagttcg gcaaggcgat ggccggcgac gccatcgagc
     1381 acgcgacgaa ggcactgctg gaggaactcg tgggtttttc cccgagcccg agagatcggg
     1441 cgaatctcca gcgggtgctc gaagcgacgt ggacggcgat ggatcgagcg agagacgtgg
     1501 tcgaagcgcg actgaccaac ggcgaactcg accgggtggt cgagcaagcg ctggcgactg
     1561 ccacgaactc atctggcggt gcgccggcat agtcggcgtc gagcccgagc ggctgacgct
     1621 ccgcgaattg ctggcgatgg ccgaggcccg catgaaggac gactggtcgc ggaccagctc
     1681 gctgatggcc ctgctggcta actgccatcg cgacccgaag aaaacgccgc cgtatcggcc
     1741 gagtgacttc gatccgttcg tcaagcgcag tcaaccgccc atggtgggcg tcgagattct
     1801 caaagacgtg tttgtgcatg gacgtattcc caagcaggag gctcacacat gaagaacgta
     1861 gcattcgcgg agttctcaat catcggtctg atccacgcgg cgctggtcgg ctgcgccagc
     1921 ctgaatcccc agccgcccat tgaggcgttc gccaacaagc tggcggacga ggcgatcatc
     1981 ccggcggtgc gcgaggggct cgcgcagggt gtcgagcaac tttccatcca agccggcgcg
     2041 caaggcatca acccgaccta cgcggtgatg ttcgagggca agtgggtggt gggcattgaa
     2101 ggccgggcga ccgtcggcgt cgaaggtgtg gcggggcaga ttcaggtctc cagcaccagc
     2161 aacgaggaga cggaaacgag cccgcacgcc acaggcggag gctcggccac gcagccggcc
     2221 aatcgggagg agccgtgagc aaacggcatc gcgggacatg gttcgcgtgc gtgtgcggcg
     2281 tgctgctcgc aggttgcaca ctgcacgtgc atctgggcgg caaatactac ctgaacgacc
     2341 cggccaagga tgagccgggg tttgtattgg aggcacaacc tcatgctgac gtggctggac
     2401 ggcaagaaga cactcatcgg cagcctgctt ctcagcctgc tgggcgcggt ctggagtttg
     2461 gacgtattga ttgacggcgc ggccaactgg ctcaccgagc agcagtacgt cgccatcggc
     2521 accaccatcg ccggcctgac cggtgcggcc atgcggttgg ccgtcggcaa ggtcgagaag
     2581 aatggcaaga cggaatgatc gacctgcgga tcaaagaact gttcttcgac cggccaatgg
     2641 tcgcgcgggc ggtgaacaaa gccaagcgtg gggtgctgtc gcgcgcgggc gcgttcattc
     2701 gtcaagccgc ccgcacaagc atccgcaagc gccaaggaac atcgtcgccg ggcagtccgc
     2761 cttactcgca caccggcctg ctgcgccggt tcatcctgtt cggctacgac cgtggcaccg
     2821 attcggtcgt ggtcggaccg gtgaggctca ataaaccggg cgaagcaccg cgcgtgctgg
     2881 agttgggcgg cacgacggtc atcgaacaac gcgtgcgcgg tcgccgcgtc cgcgcgcggg
     2941 ctcgtattca aaggcgaccc tacatgggtc cggcaatgga gaaggaactg ccgaagttcc
     3001 cggcgttgtg gcggaacagt attcggccag ggtgagcaat ggccagcaca cagggcattc
     3061 gagccggtcg agcattcgtc gaactcggcg tgagcgacaa gctcaccgcc gggttgcgtc
     3121 gggcgcagcg gcaattgcgg gccttcggcg aggggttgcg ctccatcggc acgcgcatga
     3181 ccgccgtggc cgcctcagtg ctcgcaccac tcggggcaac ggtcaaatac ttttccagcg
     3241 ccggcgacac gctgaacaag atggcggccc ggacgggcgt cagcagcgag gcgctctcgg
     3301 aactgggatt tgccgctgag caatccggcg cggacctgga aacgctcgaa ctcggcctgc
     3361 gccggatgca gaaggtgatc ggtgatgcgg ctgacggctc ggcctccgcg ggcgacacac
     3421 tcggcaagct ggggctgacg ctggcaaagc tccagggcct ggctcccgag caacagtttg
     3481 ccctcatcgc tgaccggctc agtcagattc aagacccgac cttgcgtgca gcgtccgcga
     3541 tggaagtctt tggccggtcg ggaacgcagt tgctcccgct gatgcaggat ggggccagag
     3601 gcatcgggca actgcgggag caggcccgcg cgctcggcct gactgtctcg acggggacgg
     3661 cccaggatgc cgccgtactc aacgatacgc tgaacatcct gtggcgcgtc gtgaaacagg
     3721 gcgcgttcgt ggtcggctcg gtattggccc cagcggtggt cgagctatca aacaccatca
     3781 cccgaacagt tgtttctgtc accaactgga tcagccagaa caaggcggtg gtggtcaccg
     3841 tcgccaaggt cgcagcgacc gtcgccgccg ccggcgtggc catcatcggc ctgagttggg
     3901 tggtgtccgg catggccgcg gcactaggtg gactggccgc cgtcgcatcg ggagttggaa
     3961 ccgttctggg cgtggtcggc tccgcgctca cggccttgct ctcgccgatt ggcttggtca
     4021 tcgcggcggt cgcggccctt ggtgcggcgg tcttcattta cagcggcgcg gcctgcaaag
     4081 cccttgagtg gctgcgcgag cagttcggtc ggcttcatgc tttcgtgtcc agggtggtga
     4141 ccggcatgtc cgacgccttg gccgccggcg acgtagcctt ggccgcgcgc atcctgtggc
     4201 tggccctcaa gctcgcctgg caagaaggcg tggccgccct gaaccgcaca tggcttgcgg
     4261 tcaagcggtt tatgctcacg caggcccagc agatgtggac cggcgtgttg agcggcgcgg
     4321 agttcgtctg gcacggactg aaggtcggct ggatcgaggc cacggcgttc atctcgcgga
     4381 cctggaagcg gttcaccagc ttcctggagg tgacctgggc cacgatcaag aacgttgcga
     4441 ccaagacgct gaatcacatt aagggcctgt tcgacgagac gttcgacgtg gatgcggcca
     4501 accttgtggc cgaccaggcg ctcgtggccg ccgagcagcg gatcgagcag gacaaggacg
     4561 cccaactcgg ccaacttcag cgcgaaggcc agcagaaacg cgaagccgag cagcagcgcc
     4621 acgaatcccg cctgcgcggc atcatcgagg ccgaggatga agcgctggcc gaactcgacc
     4681 aggccacaga cagtcaactc gatcagacgc ggcagcaact cgacgacgcc cgccggcagt
     4741 tggccgatgc cctggccgag gcgaaacaga aacgcgagca gatcgaggcc gaagacaagg
     4801 ccccgcaagc cggcccgcgc ggtctgctcg acgggctggc cgatcgcctg gaaggactcg
     4861 gcgacgtgat tgccaacaag attaccgtca ccggcacgtt caacccggta gcggtccaag
     4921 gcctggccgc cggcaacgac gccgcccagc gcacggccac cgcgagcgag cagacggcca
     4981 aacacaccaa gcgtctcgcg gatgcggcgg tcaccggcgg gctgacgttc gcctgataag
     5041 gaggcaccgt gccgatcacc gttgaagaac gattcgagag ccggcaggtc acgatgggca
     5101 ccaacccctc ggctgagctg cgctacaaca ttcgcggcac gaacgacgac gtggccgcgc
     5161 gggtcgcgct cgaagcggcc agcccgttgg tctatgattt ctacggcaac ggtttgtggg
     5221 tcatcccacg cgagagcgcc tcggtggagc cggtggccaa cgacatttgg gagggcatcg
     5281 ttcgctacgg catcgtgccc caggaaaacg aatcgacgtt ctcgttcgac accggcggcg
     5341 ggacgacgca catcacccag tcgctggaga cggtcgcagc ctatggcgcg gtcggcacgc
     5401 cgccgaactt cggtggggcc atcggggtga ccgacgacaa cgtcgagggt gtggacatca
     5461 ccgtccccgt ctatcacttc agcgagacgc acttcttgcc ggacatcgtg gttgccccgg
     5521 cctacaaggc gacgctgttc aacctcaccg gtcgggtcaa cggcttgccg tggaagggtt
     5581 tcgcgaccgg cgaggttctg ttcctcggag catccggcac caagcggggc ctccaggcct
     5641 gggagatcac ctatcgtttc gccgcctcgc cgaacaagtc caacatcacc gtcggctcca
     5701 tcagcggcat cagcaagaag gggtgggagt acctgtggat tcgctatgcc gaaacggaag
     5761 acactgcggc caaggctctg gtgaagcgcc cgctggccgc ctacgtggag cgggtgtacg
     5821 agtacggcga cttcagcctg atggggatcg gggtgtgagc gatgggcgag ttgaagaaag
     5881 tccaatccgg ttcgccgctg gtcatcccgg cccagacgtt caacgccttc attgacgcgg
     5941 ccagggatta ccaggaccgc caactcggcc agcagcgcac agcgctgtcg agttcaaatg
     6001 acagcggcat ggtgctcctc aagaacatca gcggcgcgga tcgcgcgcgg ttcgagattc
     6061 tgggcatcaa cacgccgctc atccagccgg ccgacaacct cgatcagttt gtcaaccggc
     6121 cagcgctgtc aggtgtgaca ccgacggcgg cgaatcactg gggcaagttc ggcgtcctgg
     6181 ccgagccgat acgcaatggc gcactcggct gggcttgggt atccggcgtt tgcccagtaa
     6241 agatcgacat cgccaacgcc aaccactggc acgccgacat cgcggacggc tcggccgcct
     6301 acctcaagag cagcggcggc ggagcggcgc aaatcctgtg gaaggactcc ggcacgggag
     6361 tcagatgggc cgtcgtgcgt ctgggccgct ggtctccgac ggtgttcccg gtgaacctga
     6421 cccagacggg tggctcgcaa ggcaccagca ccacggccgc cagttggacc tacagcgtgg
     6481 tcgatcccgt gacgaaccag acgctcgcga cgggcgtcaa tcccaccgcc tcgccccaca
     6541 aatggaagcg cccctcagcg ggctggatga tcgccgccac gttcggctac gcccactaca
     6601 acgcccaggg ccagctcgtg ctcggctgga tcaacgagat ggttgagcag gaagcatgta
     6661 ccagttaagg aagcggcacg atggccacga caggcaaggc cagattgttc aaagccggca
     6721 agcgcaccct tcgcaccaac ggcaaggcgc agttgttcaa cgccggcggc cactgctccg
     6781 aatgctgcac gccgtacgtt ctggcctcgt tcaccaccaa tagcagcacg ccctgctggg
     6841 atttgacgcc gtaccagggg ccgggcaagg cgactccctg cgctttctgg cggctgatcg
     6901 agaccggttc gtgctatccg tattcctatc cgtggtatgg gacgggatgc gtgaaccacg
     6961 ccggacaact cgtcggcctg ccaagtcagt tctgctccag ctactactac aacggctaca
     7021 tgcagctaca gatcggctgc cgctcggctt ccggcacgca gatcaactgg ccagggacgt
     7081 gccaaacgac gaccagcgtg tattcgtgtt gatctggaga agatgatgag actcgatgct
     7141 attcaccaag agaacgtgct gacgcacatg cccttcctga cccgcttggc tgcgtcttca
     7201 gacgccgagg ccatcgtcga tcaggggaaa ctcgttctgt tttactgccg atccggcccg
     7261 aaggacgtgg atgtttcggc tggttcacgg cgctggaagc tgtggatcgc ggtcggagac
     7321 cggccgccgc ggcgactgga aaccggcctg cccgacgatc taaccgagtg ctctcccacc
     7381 gcctggtctg atgacaccgg ctggcacgta acgttcatcg ccggtggcgc atcagaaagc
     7441 ccgctctacc gcctttaccg catggacgga ccggacctcg atgacttgag caagcccgtg
     7501 gccatccatg ccacccgtgc ggccttcgtg tacggcgacc ggctggtcca cgccgacccg
     7561 gagaacctga tctacgtccg ccagccgtcg ggcgatttcg acattgaact gcccggcgcg
     7621 ttcatttatc gcgtgtccta ccgcgcggat gaacccgata cacttctgat cagcggtgca
     7681 tggcagacag aacgcgacgt gttcgcgctg gagtacgatc tgaggacggg cgagcaacac
     7741 ctgctcgaat gcgacggcct gccggcctac aagtgcacca tcctcggcaa caaagttctg
     7801 tacgcccagc gcatcggcga acatttcgag catcgccgga tcaacacgtc cgaggccctg
     7861 tcacgccgcc gcgtcaacac catcgtccgt cgccagcccg gccaagtcca gccggtcgtc
     7921 acccagccga ccggcggctg cggctgccgc gggcgctcgc agaccgacgc tacccctcta
     7981 tccgccagca gtgagccacc gaaggcggaa gcttcgtctg ccatcaactc ggcggcggac
     8041 acggcacccg tcacccgcgc ctcatgcctt gagtgcatcg agaagcacct cggcgcggcc
     8101 ctggtcttgc taacagaaac ccgcgaaggc tacgcccacc gcctccgcgc catcggccac
     8161 ctgcacgaag ccgaggacga atcgcaggag ttccccaccc tgcacgccgc catccgcgaa
     8221 gcccgcaaag cctaccagac cgccggcacc atgcccgact gggaaacgct ttcccaactc
     8281 gcgcgtgagc cacagcggga agacggtgga catcgcgagc gatgagccgg tgcatcagcc
     8341 actccgcaac cgttccggta ggcactcagc atgaagccga gtgccgccga agttggtttt
     8401 tgcgcggaac ggccggaaag ggctttcaac ccggccgatt ctgacgtaaa atacaaccag
     8461 gaaaggggtt atgttgatgg cccacgcgag cttttccaac cgagccgccg gcagattcgc
     8521 aacgcccgaa gctggttggt gcgggtcatc gcccgccgcg ccgtggacat cctcaacgcc
     8581 caggagaccg gcccatgaag aagacggcaa tctacgcgcg ctactcgtcc cacgcccagg
     8641 atggcggcac gtcgattgaa gtccagctcg acgcctgccg ccgcgacctc cagcccaaga
     8701 cataccgcga atacgttgac cgggcccgca ccggccgctc gatggccggc cgtgaagcct
     8761 tgctccggct gctggccgac gccgaaaccg gccagatcga acgggtgctg gtctacaagt
     8821 tcgaccggct cggccgcaac ctcgccgaaa ccagcgcgat catcgcgcag cttgaggacg
     8881 gcggcgtcga ggtggtcagc gtcaccgagg gcagggacgc actggcccgc gggatgcacc
     8941 tcgtaatcag cgagcactat tcgcgggtgt tgtcagagcg gacgaaggac gggctgctca
     9001 agcggttcga gcagaaggct tggaccggcg gaccaccgcc ttacggctac cggatcgaaa
     9061 caacagccga tggcctgcac cggctcgccg ttcacgaaga ggaagcggcg gtcgtccgat
     9121 ggctgttcca agtctacacc agcgagtcga ttggtctgaa ggctcttgct cgccggctgg
     9181 ccaagcgcgg tattccaacc cggcgttgcc cgacgtggac gcacacgagt gttcggcgaa
     9241 tcctcacgaa cgatatctgc attggccgga tcgtttacaa ccgcagacgc ttcaagctca
     9301 acaaacggac cggccgacgg gtgccggtgt ggcgggatga gtcagagcac atcgtgcaga
     9361 gtgatgaacg cctgcggatc attgacgacg gaaccttcgc cgaagcacag aatcggctgg
     9421 ccttgcacgc gcgaccgcgg cgagacaccg gccagcttct tgccccggct taccggccgt
     9481 tcaccgggtt gatcttctgc gaaaagtgcg gcagcgtgtg ttatcggtgg accagcaaga
     9541 accgcaaggg cgagtactac tactacggat gcggctgccg tcagagaaac gggcgcgacg
     9601 cctgcgacaa catggccaac atccgggaag acctgctgat ggaccggatc aaggcgacct
     9661 accaggaggt cttcgccgat gccgactcga tcattgaaga cgccattgaa gaagcgcgga
     9721 aactggtgca atccaaccgc gaccagctcc agcgggttcg cggccagatc ggcgaactcg
     9781 acaaaaggat cggctcgatg acccggctcc tggtggaccc ggacatcgac gccacggcca
     9841 agcgggcggt atcgcggcag gtcggggagt tggaagccca gcgcgagcga ctccagaacg
     9901 ccatggcgga actggccagc gacgccaacg ataacaccgc ccggctggcc gccgcggtgc
     9961 ggcaggccct caccgaagcg caagagtcgc ttgccacggt ggccacgccg accgagatga
    10021 gggacttcat cgagcagttc gttggcccga tggtgctgac ggcggacggg gagatcgtgc
    10081 gaaaggacgc cagcgaaccg gccggaaacg acaaggcccc ggccgaagcc gaggccctga
    10141 agcggtcaat agcggggaca ggattcgaac ctgcgacctc gaggttatga gcctcgcgag
    10201 ctgccagctg ctccaccccg cagtatgtac ttgacagggt acaatcggtc tggagtgctg
    10261 tcaaggaggg tcaccgggct tggccgccgg tcgcttggcc gaccgccggc cagacaggcg
    10321 aacgagagca gcgagaaacg ggcaactgac gatcatcgct gcggtggatc aggcaacgct
    10381 tggctgcggt gaaccccgtc aaccctttcc cgactcggtt gggatcgaac gaagactcag
    10441 cattggcccg ggatttgatc ggacattccg actcagcctc cgtggccgat gagtttgggc
    10501 accagccgcc tcttctgttc gaatacccag cggtagtaat ccgcccgttt gagcttggcc
    10561 gcagcgggct gatcgaggag aacggtcacg tccggatgca gttgcagggc actggcggtc
    10621 acctgcgagg taacgggacc ttcgacggcc tcggccacga tatcggcctt ggaggcccca
    10681 aaggccaaca ggatgatctg ccgtgcttcc aggatcgtgc cgacgcccat ggtgatggcg
    10741 aaccgcggca cttcgtccat gctgctgaag aagcgggcgt tgtcgcggat ggtctgctcg
    10801 gtcagggtct tgaggcgggt ccgggagccg agcgatgaac cgggctcgtt gaaggcgata
    10861 tgcccgtcgc cgccgattcc aagaatctga atatcaatgc cgccgctccg cttgatctcg
    10921 tcctcgtacc aggcgcagaa atccaggacc gagtcggcat gcccgtaagg gacatggatc
    10981 gcgctggccg ggacgttgat gtgcttgaag aggttttcct ccatgaagta acgataactc
    11041 tgagggtggc tcggcggcag accgacatat tcgtcgagat tgaacgtggt gaccttggag
    11101 aagtcgagcc ccttctcgcg atggcgcttg gctagctctt tgtacaaccc gaccggcgtc
    11161 gaaccggttg ccagccccag gacggcggca ggcttggaca tcaacttgcg ttcgatgata
    11221 tcggccgcga aagcactcgc ctcgtcggcc gtcttggtaa tgatgacttc cattgcgttc
    11281 tcctgttaca taacaaagcg acggcgtcgc ccccgatgcg acgagccggc gacgggggga
    11341 cattataacg gcgtgcttgg gcaaatgaag aggcaagcac ccagggggaa ggcggcctgc
    11401 cggccggtgg cagcgcgtct ccgaaccatg gagaacgcgg agctttgcac gctgcggttt
    11461 cctgattcat tgtgacgctt cgagacgatc gcagtgaccg tcgcgctatt gcgaaagcgg
    11521 catgccggca gtataggtgt taggctcaat cggccattga tgctttcttt tatgcttgga
    11581 gacggttcat gaagctcgtc gtttttgagg acagtcagtt cgatcgtttt ctgccgctgg
    11641 cctgggtccg gggggttttt gagctgaaga ccggggcgac tacgctccgc gagaagatcg
    11701 agcgggccgc cggcaccaag gccacggccg tcatcgctcg ccattacctc gctgcctgtg
    11761 tccgccggcg cagcggttcc gcggccgtca acgaccttcg cggctgcaag ggcgacgagg
    11821 tgctcttcgt gaattcccgc gtgcgggcgt cgcagtggac gccgcccgcc aaggacgggg
    11881 cgcagtggca cggcgaccag ttggccgtat ggcggacgag ggacgacgtc agcgggatca
    11941 ccgactacga cggcctggtt gcggcggcga gcaggacgcc caaggaggag tacaccggca
    12001 aatggttcga ctacatttgg gacgtcatgc tgtgcaaccc ggaggagatc gttgcggatt
    12061 tcaaggctgc cggccgcaac ggcgttcacg gcacgatgca tgagtcgtcc gtgatctacg
    12121 gcccgcgcga gcaggtcttt gtcggcgaag gtgccgagat tcaccccttc gtatgtattg
    12181 actcgcgtca tggtccggtc accttggagg caggggttga ggtacacccc ttcacccgca
    12241 tcgagggacc ttgctacgtg ggcgccaata gcattctgct tggggccaag gttcgggagg
    12301 gatgcagcat cggtccgatg tgtcgtgtcg gcggagaggt cgaggagagc attattcacg
    12361 ggtactcaaa caagtatcac gacggctttc tgggccacgc gtacgtcggc gagtgggtca
    12421 atcttggcgc cctcacgacc aacagcgacc tgaagaatga ctattccacg gtcagcatga
    12481 tgatgggggg ccgaaccatc gataccggtt cgaccaaggt cggcagcatg atcggtgatc
    12541 acgtgaagac gagtatcggc actcttctga acaccggcac gatcgtcggc acgatggcca
    12601 ttctggtggc caccggggcc ccgctgccga agtatattcc gccctttgcc tggttcctca
    12661 acggcttcgt cgggaagggt ttcgggttga atgtgctgat ctcgacggcc cgcacggcca
    12721 tgtcgcgccg gaaggtcgag ctgaccgcgg acgaagagca gctcctgcgg cacgttttcc
    12781 agctcacgga cgaggagcgc atgagttatg tccgcaaggg tcgccgggcg atgctcaaga
    12841 agtaagcgat ccggcgttga ttgggcagtt gccaggtttt taccgtcacc tgaacccgca
    12901 ggtgatgatc cgcatcctca tgatcggggc ttgatgcgac aaggagggct gctaaactga
    12961 gctttgtgag gtcgcgaccg gcgatccgcc ggtcagattc accgtgcagg atcgcggcga
    13021 cgcaggagtc tgccccagcg ggaagagagt ccctgtgtgc gcgtggatgg gatacaggat
    13081 ggaatccggc cggccgccgg actttggagg caatcggatg gcagatcagg acgtactcaa
    13141 tcgtgtgatc aaagtgaccg ctcgtgtgtt gaacctcgaa ccggagacga tcaagcccga
    13201 gcacagcttc gctttcgatc tgggagccga gtctgtgcag agtgtcgagt tggtcgctgc
    13261 ctttgaggag gaattcggca tcgagatgga cgaagatgcc gctctggccg tggccaccgt
    13321 cggggccgca gccgatttca ttggcaaaca cttgaaaggc tgatcgacgg tcggcacgag
    13381 gttcccggac tcctgatacc gactgcttca ttctgctcgc acgggcagcc gtcgcatggc
    13441 cggcgacggc cgacggagcg ctctgcgggg acgcacggca tccatgtctc cgcagacgcc
    13501 cgggtgatgc gcatcacgcg gccctccggg atgcggcaaa gacccgcatg gcggccgagt
    13561 cgggtgctct cgtcgctcga catcgagttg ccgacgtctt gcctgttcca gcagaatggt
    13621 ttagtgtctg aagtttttgg ggctgtatcg atagaagcgt tattgtcgaa tggagaacca
    13681 tgtcagagcc ttcgatttcc tcagaaacgt ccgcgttgat cagttcttcc ggcgccggtc
    13741 ttgacaccat cttccgtccg cggtccatcg cggtgttggg cgttacgaac acgccgggta
    13801 ccgtgccgca cgacattttc gtcaatctgc tcaacgggcg attcaacgga ccggtttatc
    13861 cggtggcccc gcgcaagcgg cacatcgccg gggtgaaagc ctacgactac gtgctggaca
    13921 tacccgaccc catcgacatg gccatcctgg tttttccggg ccgggtgtgc gagttggcgc
    13981 tggagcaatg cggacagaag ggcatcaagg cggcaatcat catctcggcc ggtttccgtg
    14041 aggtgggtcc cgcgggtgct gagcgggagg agaacatcaa agccatcgcc cgcaaatacg
    14101 gcatgcggct cattggcccc aactgcctgg gggcgatcaa caccgaaccg gacgtgcaga
    14161 tgaacgccag cttcgcgcgg gccatgccgg cggcggggag tatcgcgttt atttcgcaaa
    14221 gcggcgcgct gtgcacggcc gtgctcgact acgcccaggg catgaatatc ggcttctcga
    14281 aattcgtcag cctgggcaac aaggcggatg tgaccgaggt cgatctattg tcgtatctgg
    14341 ccgatgatcc gcagacctcg gtcattctga tgtatctgga ggagatccgg cgagggcgtg
    14401 aactggtgca ggtggcccag cacatcacgg cggccagcaa gacccctaaa cccattctgg
    14461 cgatcaaggg cggacgaacg gcccaggggg cggcggcggc ccagtctcac accggggcgt
    14521 tggcttcgtc tgacgtagtg tgcaatgccg tcttcgagca ggcgggaatc atccgctcga
    14581 agagcatcga ggagatgttc aataccgccc ggttgttggc ctaccagccg ctgcctcggg
    14641 gaaaccgcat cgcgattgtg accaacgcag gcggcccggg tgtcatggcg acagatgccg
    14701 cgatcgagcg cgggctggaa ctggcgaaat tcgcccccga gaccaaggcc aaactgaaaa
    14761 aggcccttcc ggcggcggca aacatgaaga acccgatcga cgtgatcggt gacgctcgtg
    14821 acgatcgcta tcgagccgcc atggatgcag tttacgaaga ccccaacgtc gatcagatac
    14881 tggttattct gacgccgcag tcgatgacga acatcaccac aatcgcacag gccgtctgcg
    14941 aagaacagaa gcgccacgcc gacaagcaaa aaccgctgat gtgttcattc atgggagcca
    15001 aagacgtggc cgccggtatc gcgattctgc aaaaccatgg tatcccacac tacgtgctgc
    15061 ctgagtgggc gaccgacgcc atggaagatg ccgtccgata ccgccggtgg cgcgatcgca
    15121 aacgcacgag gatcaggatt tatgaagacg tggatcggga cacggccgcg tcgatcatcc
    15181 gggaggcctc cgacggctac ctgatcgagc cgctggccct taaggtgctg gcgtcctatg
    15241 ggctgcccgt tgttccttcc gagctgaccc aaacggcgga cgaagcggtg gctgcggccg
    15301 accgcctcgg atatcccgtc gtccttcgcg ttgtgtcgac gagcatcgta cacaagttcg
    15361 atgtcaaggg cgtgatgctc aatctcagga acgctgacga gctgcgcgcg gcgtgccggg
    15421 aaatgcgcga ggccattctg gagaagtata gcgccggtga tatcgccggc ttcctggtgc
    15481 gtaagatgat cccggccggc aaggaggtca ttcttggtct gagccgcgac cccgtgttcg
    15541 ggcacgtcat catgtgcggg ctgggaggaa tctacgtcga ggcgctcaag gatgtgacct
    15601 tccgaatcac gcccatcagc gaggccacgg cgacgcgaat gatcaacagc ctgcgaacca
    15661 ccgccgtgct caagggcctg agaggcgaag ctcccagcga catcccggcc attgaggatt
    15721 gcctcaagcg gctttcccaa ttggccactg attttaccga tatcgctgaa ctggacatca
    15781 acccgctgat cgtccatccg gccggccagg gatgtcacgt ggccgacgtc cgcatccgta
    15841 ttcagaagct gacgtgacgc gaatactcac acccggttct catttcaacc gtccggttct
    15901 ctgcctttgc gactccctcg acaaggcgct gaccggcgcg accaaggctg ggccatcttc
    15961 agtctctcga ccggcagccc gcgcaggtcc gccatatccg agccggtgga ttctctccgg
    16021 gtttggcacg cggtcgggca acaacatgct ttcttggtcg gctgccttga cagttgcgag
    16081 ccggcttctc tacaatcccg ctatgctaaa ctcaattaca tcaatcactt ggcgtcttgc
    16141 cctgcttatg ttcgctggtg cgatgttggc tggatgcaac ggcgggccgt cgcggattcc
    16201 caccgaaccc cagcgccggg caatgctgtc gttgatgctc cccacgcaca ttaagattca
    16261 gcccttcaca cgtatagcca gtttcgacaa agacaacatc cctgacggcg tgattgtcgt
    16321 gcttcgggcg attgatcgtt tcggagaccc ggtcaaggcc gctggaccgt tttacttcga
    16381 gctttggtcc taccagccgg cctcaggtga acccaagggc cagcgactgg ccttctggga
    16441 tcagaccctc gacaccgcgg acaaggtctc cggacactgg acccatgcgc agatgtacga
    16501 gttcaagctc ggctggcctg aaggtgccac ttccgtccgg ccgggcagca agtacgtgct
    16561 gacggcgact taccgcacgc cctgggatga gactatgacc gacgagtatg tgctggactt
    16621 ctcagtgccg ccgggagcga ttcagagagc gatcgaaaga aagcctccgc agaccgcgcc
    16681 ggggatgtga gagagaacca tcatgtgtcc gctctcggtc gtcggcccgc acggaacctt
    16741 gccccggcag gacgcgccgc cgtgaaccct cagcgcgggg ttgggctgat gttttctctt
    16801 gattcatcct ttggccctta tcccttgacc ttcatcaacc cagtcctatc ttccccactt
    16861 gagtttcgtg gtcagcgtgt gccattttga ataggccggg ttgcgaacca actggaaatc
    16921 agcgggccac cgacgaacag tgatgcgatc acccgcgtga accggcaacg agacctggcc
    16981 gtcgatcacc agcgtcgtac cctgattgac gctgtgcgcg acaacctcga tgaccgaatc
    17041 acccgcgaag accaggggcc gatgggtcaa cgaatgggca ctgatgggtg acaggaccat
    17101 ggctcgggtc tcgggttgaa gaataggacc accgaccgac atgttgtgtg ccgtcgagcc
    17161 gcttggagtt gccagcacca agccgtcgcc gctgatgttg gtcagatggt cgccgttgac
    17221 cgataccgaa agctcgacca tacggaaggg cggcccggcc tgcacgacgc agtcgttgac
    17281 cgcaaccgac tgagcaacga gtctcgcgtt gtcggcaatc tctgccgcga tcatcattcg
    17341 cggacttacc gccgccgagg cactcaccgc ggcatccagg tttctgtcca gctcttcgac
    17401 ggtaaactcg gccaggaagc cgagtttgcc gaaattgacc ccgatgatcg gtacctgccg
    17461 ttccttaagg gcgcgaacca cggccaggat actgccgtcg cctccgagca cgatcagtcg
    17521 atccggcttt gactctgcca acctggccgt gtcggcgatg ggccctgttg ccaccacatc
    17581 gatccgctga cgcaagtcct ccagcaacgc cgtctttgtc gcctccacat gaggcttgtc
    17641 cgggttgtag accactacca cgcgcggccg agccattcgc cacctccggc ccccagaggc
    17701 catttgacga gcagtattat accgtcgctg gcggcccgct gctggttcac ctcgtcgggc
    17761 aggcaaatcg gctggagcga acagcgcgcg caacgcgctg acaggcggga agccgtcatg
    17821 ggcccgccgt catgaattga acgcttctcg ctgtgtaagg gcgcagctcc cgccaagggc
    17881 cgtgccgaca agcaaagtgc cttgagatta gcccaacaac cacagcaccg ccacggcccg
    17941 agtcattgtc cctgaaggac aatttccagc gggtggccag tcgtggcggg gaaggtcacg
    18001 tgagtcgggt ccgtggagtt tggggccgtc agggtcaggt ggtgcgttcc gggttgggct
    18061 g
//
LOCUS       DAOTPW010000111        18012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3138_length_18012_cov_5.621763, whole genome shotgun sequence.
ACCESSION   DAOTPW010000111 DAOTPW010000000
VERSION     DAOTPW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18012
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3138_length_18012_cov_5.621763"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1362
                     /locus_tag="P5316_11500"
     CDS             121..1362
                     /locus_tag="P5316_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR85623.1"
                     /translation="MGEIQHELMPRGNPGEPFIRRVRIRNYKSLGKCDIQLRPLTILV
                     GRNGSGKSNFLDTLRFVAESLKGSLDHAIKARGGIDAVRRRSTGHPRNFAIELEMALP
                     DWHEARYGFEIGARPRGGFVVKREYLKVFKANSNLAAEYSVREGTLVSQSENMPPVSP
                     DRLYLVVAAGLPAFRQVYDQLTSMGFYNLNPDAMKELQSPDAGDLLHRDGSNIASVIA
                     RLGSDQPQVKDRIRSHLCTIVPGVTDVDRVSLGPRETLEFRQEIAGSDLPWKFYAASM
                     SDGTLRALGTLVAVTQLADRTHPASLIGIEEPETALHPAAAGALMDSLQEAATRTQIL
                     LSTHSPDLLDQLDPATTGLLVAQARQGTTEIASADAASLQSIRNHLYTAGELLRMDQL
                     QPDSEDLRRQLEIDFGDEGDL"
     gene            1359..1976
                     /locus_tag="P5316_11505"
     CDS             1359..1976
                     /locus_tag="P5316_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HRR85624.1"
                     /translation="MSRLRVAPIVEGYGEFQAIRPLFMRLWTEQLGGEYIDVLQPIRR
                     PRSQLGTQEGLSRAISLAVLKLTTGQPSPDPCLILVLLDRDPDPKPPCELGPKLLESG
                     LAARPDFDITYVLANIEYETWFVAAAESLRDYIEMPPDEPVPSSPEDARHGKSWIQRH
                     FKGIKYSETVDQPAMTSKMDLALCRRRSPSFDKLCRELEKRLKSG"
     gene            2011..3285
                     /locus_tag="P5316_11510"
     CDS             2011..3285
                     /locus_tag="P5316_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85625.1"
                     /translation="MSDSRFSRRKLLSGTTVAAAGLAAVGGCAATRGTGLAAGASAAK
                     SRRIGPNDRVSIGIIGCGGRGSQLMQDIHDVAKDRNAEITAVCDVWKVNLNVAAAKVK
                     EWFGKQPRASTRFGDILALDDVDAVVIATPDFGHTPIMIEALKAGKDVYVEKPMSMEI
                     AEANQALDLARKHERVVQVGTQRRSEGRFKGAAKEFSTGILGKLNRISAAMNVNHPRW
                     ARSYADCKQSDVDWDAFLFNRPKRPFDPSLLRQWHFYKLCTNGLSGLWMAHYSDSVHM
                     VTGAKYPTSAVAHGGIYVWKDGREHTDTFHALLDYPEGFLMSWGMGLANSHGVHFDLC
                     GTEATMDLEKWVVSGEGGAGVKKMPARPLQAEPSESHMGNWIDCIRSRKRPNADIEYG
                     HQHAVATIMAAAALETGRRHVYDPVRREMRPG"
     gene            complement(3296..4060)
                     /locus_tag="P5316_11515"
     CDS             complement(3296..4060)
                     /locus_tag="P5316_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85626.1"
                     /translation="MGQWQPEVESQMVEEYRQSQSRRWPRRVVALAFLLLGCACLSTC
                     DRRNTDPSALGLPPTASPKEVYEQLRKWHASHSYGSMAPWVHPDSSDELIELLISIDE
                     MMTANTTVQVALAQYCPHIDPRQFDLPAIGYFLELFAQDARFVSETINGDRAVVAIQV
                     AGRLPLRYPEFGRYEGRWVYMPGKVSREMVRGVRDLAKGLTRFAAAITAGARTQKQLD
                     SEFRYRVAGKLETFVREAAPTSAPSPWRVMAATLPS"
     gene            complement(4407..4961)
                     /locus_tag="P5316_11520"
     CDS             complement(4407..4961)
                     /locus_tag="P5316_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRR85627.1"
                     /translation="MGSLSPPDLRSDEELIRAINQGDTAAFETLYYRYRDWVVRLATR
                     FSGRTDDALDVLQDTFAYLLRKTPSLRLSARMTTFLYPVVKHLSLAARRRSCRSLGCE
                     LDADVPAPPPSENDGARSSLAEVMAGLSPDQREVVLMRFVDDMTLAEIAQALRMPVGT
                     VKSKLHRALERLQNDPRTRAYFQR"
     gene            5278..7662
                     /locus_tag="P5316_11525"
     CDS             5278..7662
                     /locus_tag="P5316_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRR85628.1"
                     /translation="MKTSVKSLGIVVPLLALLALAAGGPVPRAMADGFIVVDDRSVEE
                     IRRLWPHPRPVPRPTPWRYHMPLQVKNHLVEAELTDSVGVTRIDQTFHNPNNVQLEGT
                     YIFPMADDVAVQKFSMMIGDKEVQAEVLDKDRAQQIYEEIVSKMKDPALLEYIGSRMY
                     RARVFPIPANGDVRIKLEYSQVITATNGLATYRYPLNTEKYSSAPISQVAIRARIKTR
                     QPLATVFCPSHQASIDRKGDNEASVGYEASNVLPDKDFVISYKTSNEDFAFVLLTHRQ
                     AGEEGYFMARIIPGEGARPRKVVAKDICFVIDTSGSMAGKKIQQAREALRYCLANLNA
                     DDRFNIVAFSTEARPYRDVLVPAKPENVETARQFAAELQAAGGTAIHDALLAALKARG
                     SSDEDKGRPYMIVFLTDGLPTIGETNIDRILENVKKANAGGVRLFVFGVGNDVNTKLL
                     DKLAEDNHGSRDYVAENEDLELKLSNFYGTIANPVLSDLKLTFDSGLGVHDLYPKVLP
                     DLFKGGELVIFGRYANPGKGKVELAGRCGDEDLKRSWEESFPQTDAGQEYLPRLWATR
                     KVGYLLDEIRLHGENKEVREEVVQLAKRFGIVTPYTSMLIMEEDEGRLARGESDRIEG
                     LTLHRRLVKARPRPPAPARPRDSGRLFSMDAGDEAVAMSKEAKRMAVAERPHEMDSYF
                     GGYARGSVGREPAAGGVASLGDAARYGYAPASQPAGQEDEQVMRYVGSRTFYRDGEKW
                     VDSRYKKELQTTTVKAFSKEYFELVAAHPDLGKCFALGSKVIVVLGDKAYETTE"
     assembly_gap    7792..7891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8262..9161)
                     /locus_tag="P5316_11530"
     CDS             complement(8262..9161)
                     /locus_tag="P5316_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRR85629.1"
                     /translation="MSLNHDDTRPVSEKASEDGKKVGGRQVRVAAIQMLPATGDPKAN
                     VERADRLVREAVQKHRAQLVILPETTLTGYTSPSDKGLTLAQSRELAEPVPGPSVNHF
                     ARLAAELKVYIVWGLHERSGDKYYNAAVLLSPKGEILGTYRKVHINKYESHMGWTNGD
                     RFQVWPCEIDGVSFNLGIMICFDREVPEAARCLTVLGADIIAIPQATGCTCEIPIHRD
                     QLRVRAYENEVYIAMANWAGPVFKGHSMIIAPSGEVIRLGTRDEEILDATFDLDALKK
                     HRENGIYGRHHRRPAVYGPLLEQ"
     gene            complement(9389..10039)
                     /locus_tag="P5316_11535"
     CDS             complement(9389..10039)
                     /locus_tag="P5316_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRR85630.1"
                     /translation="MTNNGQSTAVSPPTAKPAIPRWHLHRRMYDWVLHWADTPYGAIA
                     LFLLAFAESSFFPVPPDVLLIALVLGSRARWFRFAMTCTLASAIGGIAGYGIGLAFMD
                     TIGTRIIAFYHAESYYQQVTQWYQQYDYWIVFIASLTPIPYKVFTIASGAFHMNLLGF
                     VIVSIVGRGARFFAVSALLYFFGPPMKRFIDRYFNLLCVVFVILLVGGFAAIKYVR"
     gene            10317..10517
                     /locus_tag="P5316_11540"
     CDS             10317..10517
                     /locus_tag="P5316_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85631.1"
                     /translation="MSTLFLSMPLKNKKLAISRVWIIGIVMPVVLFPFHERVINSTDT
                     NEQANKPSFFGVGGGFSAFITR"
     gene            10978..12099
                     /locus_tag="P5316_11545"
     CDS             10978..12099
                     /locus_tag="P5316_11545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR85632.1"
                     /translation="MSIPLVNLKRLHDKLYTEIRTAINRVLAQGDFITGSDLAAFEEE
                     FAAYCETKYCVGVGSGLDALTLAMRGLGIGPGDEVITTSNTFIATALSIYHAGATPVL
                     VDHDPETYNLDPRRIAAAITPRTKAIVPVHLYGQPAEMDMIQAIADEHGLLVIEDAAQ
                     AHGARYKGRRAGSMGRAGCFSFYPGKNLGAMGDGGAVVTNDEDLAAWLMAARNYGSTV
                     KYYHTMPGYNCRLDSMQAAILRVKLRYLDEWNATRRQLAARYREKLAGANISPPVERP
                     HVDHIYHLFVVRCNQRDQVLKHLNSKGIGAGIHYPVPIHRQVGLSTRCIVPRPLVYTD
                     RYCDQLLSLPMCPCLTEADMDIVVRELSAVTATAPTVTA"
     gene            complement(12349..14163)
                     /locus_tag="P5316_11550"
     CDS             complement(12349..14163)
                     /locus_tag="P5316_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRR85633.1"
                     /translation="MILQPPDDEALRRNGTRRSSRATLAFIALVVDAAFVMAPGCQFP
                     SANSDIVPDQLIVSLRPGATQGDLVNLLNGHDAAVNGQLQGLSAYLVGVDPRNREQLA
                     RSLRGSPLVEEVLNNRLIQVEITPNDPDYTIQWFLQTVHVPQAWTLTAGSGNITVAVL
                     DTGVDASHPDLADKLRTGANTFDTGLSYADTSGHGTAVAGIIGAQSNNADGAASVAPG
                     CPILPIRVTGPDNKADSWSIAAGIALAVNRGAKVINISFAPLHENDIVLRQARMAWVS
                     GCLTVIAAGNGGGRVEAVGSDEVLFVGAIDQFDKLASFSTSGDFVDLVAPGVSIYTTT
                     LGNAYAAWTGTSFAAPIASGIAALIWSVNPNLRPATVRDILLATARDLGPTGWDETFA
                     FGCVDAATAVELAVRIVEQQDTTPPTIAIVSPAGGTAVSSPTVLQVEAGDALGIAEIV
                     LYVDQTRKASDSVWPYGFVLDPGKYATGVHELRVKAVDLAGNVSEAAINVTITSAPDG
                     IPPIVDITSPRSQTTVRGMVSIVARATDDRALSRIEILIDGNSVLSLEPSDSGATVAH
                     NWNTADPRVALGSHTITVRATDASGNAATSSVNVTVAR"
     gene            complement(14385..15629)
                     /locus_tag="P5316_11555"
     CDS             complement(14385..15629)
                     /locus_tag="P5316_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR85634.1"
                     /translation="MDFIPITRVRRTFPADRLTDIEASVSEELIHCGVNLQPGSRIAI
                     AVGSRGIAELDRFVRAVVQWVRRCGAEPFIVPAMGSHGGATAEGQRAVLAAYGVTEEN
                     VGCPVRSSMEVVELPQGDLPTKVFLDRQAASADNTILINRIKMHTSFRGRYESGLMKM
                     IAVGLGKHAQAQALHRLGVDGLREMMPRVAGRILTHGNILLGLAIVENAYDEPMLVRA
                     IRAPDIPAEEPALLDIARANMPALPVDRLDVLIVDQIGKDISGLGMDPNVIGRLKIRG
                     QPEPKSPDIRVITIHDLSSASHGNAIGMGLADIITRRLFDKIDFTSTYANVLTTTFLE
                     RAKVPIIADTDREAVRMALQACPVSRPDEVRLIRIRNTLRLDDLQVSLPVLNEIRTLP
                     GIQVSGSVESLFDSAEALRPWT"
     gene            16369..16450
                     /locus_tag="P5316_11560"
     tRNA            16369..16450
                     /locus_tag="P5316_11560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16403..16405,aa:Leu,seq:tag)
     gene            17011..17982
                     /locus_tag="P5316_11565"
     CDS             17011..17982
                     /locus_tag="P5316_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR85635.1"
                     /translation="MMNTLISRVSVLVSAGFLAGTAAAVSIETVPVGNVGNAPDRRYG
                     GYGSVGYTYNIGKYEVTAGQYTEFLNAVAKTDTYGLYNPNMWSSSYGCKIERSGSSGS
                     YTYSVAQDWANRPVNYVSWGDAARFANWLHNGQPTGPQGLSTTEDGAYYLNGATSSDA
                     LLAVTRKPDWKWAIPTEDEWYKAAYHKNDGATGNYFDYPTSSDSAPSNQLSDPDPGNN
                     ATYYNNGWTIGKPYYRAEVGAHENSDSPYGTFDQGGNVWEWNEAVIGASRGLRGGSFY
                     SFDPDLLASARYSGDPLSEECLDGFRVVEVPEPAIMMMMALADLSDL"
BASE COUNT         3595 a         5202 c         5808 g         3307 t
ORIGIN      
        1 ttttttacga acaaagcgag aaaacctcgc cggagtatgc ttgatcaggg gctagacttc
       61 tcagccggtg ccgaacagag ggagagatgc ttgacttgtc gtagggtccc gaggttttcc
      121 atgggcgaga ttcagcacga gttgatgccc cgcggcaacc cgggcgagcc tttcatccgg
      181 cgggtcagga tccgcaacta caaaagcctg ggcaagtgcg atattcaact gcggccgctg
      241 acgatcctgg tggggcgaaa cggctctggg aaaagcaact tcctggatac tttgcgtttc
      301 gtcgctgaaa gcctgaaggg atcgctcgat catgcgatca aggcgagagg aggcatcgat
      361 gccgtccggc gacgcagcac tggacatcct cgcaactttg ccattgagct tgagatggcc
      421 ttgcctgatt ggcatgaagc ccggtatgga ttcgagatcg gtgcgcgccc gcgaggcggc
      481 ttcgttgtca agcgagagta tctcaaagtc ttcaaagcca acagcaacct cgccgccgag
      541 tactcagtaa gggaaggtac tctcgtctcc cagagcgaga acatgccgcc tgtctcgccc
      601 gatcgactct atcttgttgt tgccgccggc ttacctgctt tccgacaggt ctacgaccaa
      661 ttaacgtcga tgggcttcta caacctgaat cctgatgcga tgaaggaact acaaagcccc
      721 gacgcgggtg acctgctcca ccgggacggc agcaatattg ccagcgtgat cgccagattg
      781 ggttccgacc agcctcaggt caaagatcgg atcaggtcac atctgtgtac gattgttccg
      841 ggcgtcacgg acgtggaccg ggtgtcactg gggccccgcg aaaccctgga gttccgccag
      901 gaaatcgccg gttccgatct cccatggaag ttctatgccg ccagcatgtc ggacggaacg
      961 ctgagagcac tgggcacgct cgtggccgtc acacagttgg cggatcggac gcaccccgca
     1021 agcctgattg ggattgagga acccgagacc gctctgcacc ccgctgctgc gggtgctctt
     1081 atggactctc ttcaggaagc cgccacacgt acccagattc ttctgagcac gcacagcccg
     1141 gacctgctcg accaactcga tcccgctacg actggactgc tggtggcaca ggcgcggcag
     1201 ggtaccacgg agatcgcgag cgcggatgcc gcaagtcttc agtccatcag gaaccatctc
     1261 tacacggccg gagagttgct tcgcatggac cagttgcagc cggattccga ggacctgcgg
     1321 cggcagcttg aaatcgattt tggagacgag ggggacttgt gagtcggctg cgagttgctc
     1381 cgatcgttga ggggtatggt gaattccaag ccatacgtcc gctgttcatg cgactgtgga
     1441 cggagcaact cgggggcgag tacatcgacg tcttgcagcc catccgcagg ccgcgatccc
     1501 aactggggac gcaagagggc ttgtcgagag cgatatcgct ggccgttttg aaacttacca
     1561 cgggccaacc aagtccggac ccttgcttga ttctggttct gctcgatcga gatcctgatc
     1621 cgaaaccgcc gtgcgagttg gggccgaagc tgctggagag tggactcgcg gcaaggccgg
     1681 atttcgacat cacctacgtt ctggcgaaca ttgagtacga aacctggttt gttgccgccg
     1741 cggagtcctt acgcgactac atcgagatgc cgccggacga gccggttccg tcctcgccgg
     1801 aagatgctcg gcacggaaag agttggattc agcggcattt caagggtatc aagtacagcg
     1861 agacggtgga tcaaccggcc atgacctcga aaatggatct ggcattgtgt cgtcgaaggt
     1921 cgccgtcgtt tgacaagctt tgccgagagc tggagaaacg tctcaagtcc ggttaaggat
     1981 cgaaaagtaa tcgctacttg gaggaatccc atgtcagata gtcgattcag tcgtcgcaag
     2041 ctcctgtccg ggaccacagt tgccgctgcc ggtctggcgg cggtcggcgg ttgcgcggcg
     2101 accaggggca ccggtctggc cgcgggagcg tccgcggcca agagccgtcg aatcggcccc
     2161 aatgatcggg tttcgatcgg catcatcggc tgtggtggca ggggcagcca gttgatgcag
     2221 gatatccacg acgtggccaa ggatcgtaat gccgagatta ccgccgtttg cgacgtctgg
     2281 aaagtcaatc tgaacgttgc cgccgccaag gtcaaggagt ggttcggcaa gcagccgcgg
     2341 gcgtcgacgc gattcggcga cattctggct cttgatgacg ttgacgcagt ggtgatcgcg
     2401 acgcctgact tcggccacac gccgatcatg attgaggcgc tgaaggccgg caaggacgtt
     2461 tacgtggaaa agccgatgtc gatggagatc gccgaggcca accaggcact tgatctcgct
     2521 cgtaagcatg agcgagtcgt ccaggtaggc acccagcgtc gcagcgaggg ccgcttcaaa
     2581 ggggcggcca aagagttctc taccgggatc cttggcaagc tgaacaggat ctcggcggcg
     2641 atgaacgtga atcacccgcg atgggcgcgg tcgtacgccg actgcaaaca gagcgacgtt
     2701 gactgggatg ccttcctgtt caaccggccc aagcgcccat tcgatccgag cctgctccgt
     2761 cagtggcatt tctacaagtt gtgcaccaac ggtctgtcgg gtctctggat ggcccattac
     2821 tccgactcgg tccacatggt caccggcgcg aagtatccga ccagcgcagt tgcccatggg
     2881 gggatctacg tctggaagga tggtcgcgag cacaccgata cgttccacgc actgctggac
     2941 tatccggaag gtttcctgat gagttggggc atgggtctgg ccaacagcca tggggtgcat
     3001 ttcgacctgt gcggcaccga ggcgaccatg gatctggaga agtgggtcgt ttcaggcgag
     3061 ggcggtgcgg gagtcaagaa gatgccggcc cgtccgcttc aggccgagcc gagcgagtcg
     3121 catatgggta actggatcga ctgtatccgc tcccggaagc gaccgaacgc cgacatcgaa
     3181 tacggacatc agcacgccgt ggccacgatc atggccgccg ccgccctcga aaccggccgt
     3241 cgccatgttt acgacccggt caggcgtgaa atgaggccgg gttaatcacg attgctcacg
     3301 aaggcagagt ggccgccatg acgcgccaag gggatggggc gcttgtcggc gccgcttcgc
     3361 gcacgaaggt ctccaatttg ccggcgacgc gatagcggaa ctcgctgtcc agttgcttct
     3421 gtgtccgggc ccctgccgtg atcgcggcgg cgaagcgtgt cagccccttg gccaggtcgc
     3481 ggacacccct taccatctcg cggctcacct tgccgggcat gtagacccaa cgtccctcat
     3541 atcgtccgaa ttccgggtat ctgagcggca gacgtccggc cacctggatc gctaccactg
     3601 cccggtctcc gttgatggtt tcactgacga acctggcatc ctgggcgaac agttcaagga
     3661 agtagccgat tgccggcaag tcaaactgac gggggtcgat gtgcggacaa tactgggcca
     3721 gcgccacctg cacggtggtg ttggcggtca tcatctcgtc gatggagatc aggagctcaa
     3781 tcagctcatc actgctgtcc ggatggaccc acggtgccat gctcccgtat gagtgcgaag
     3841 catgccattt ccggagctgc tcgtagacct ccttgggcga tgccgtcggg ggcaggccaa
     3901 gcgcagacgg atcggtattc cggcgatcac aggtgctcag gcaggcgcag cccaacagca
     3961 gaaacgccaa tgccacaact ctccttggcc atctgcggga ttgtgattgt cggtactcct
     4021 cgaccatctg cgattccacc tcgggctgcc attgtcccac gtacaagatt cgtccgcgtc
     4081 acctgagact cagcatcatc tgtccggcat cgcgagtcgg tttccggcgg gcgagccttc
     4141 tgggaagccc tgaaggcatc ccgctcatag atactataca cctgagccgg cctggggctg
     4201 ccaaagagga gccgcccttc ggacaaaccg ctgtcgccat caggttcgcg ggtggagcga
     4261 tcgtactccg tccggcaatc cgatccaccc agcgcggctt ggtggaccgc ttttctcggt
     4321 cctgaaggaa cagggccgtc tgcaaagcgg cgggcggtaa ggcaccggga aaagccggcg
     4381 acatcgcttt tccgcgcagc ccatctttat cgctgaaagt aagcccgcgt gcgaggatcg
     4441 ttctgcaatc gctctaaagc ccggtgcagc ttggacttga cggtcccgac aggcatccgt
     4501 aatgcctggg cgatctcggc cagggtcatg tcgtcaacga accgcatgag aacgacttct
     4561 cgctggtccg gggacaagcc ggccatgacc tcggccaagc tgcttcgagc gccgtcgttt
     4621 tcggacggcg gcggggccgg gacgtcggca tcgagctcgc aaccgagcga acggcagctt
     4681 cttcggcgag cggccaagct gaggtgcttt acgaccgggt agaggaacgt cgtcattctg
     4741 gcggaaagac ggagactcgg cgttttacgc agcaggtagg cgaaggtgtc ttggaggaca
     4801 tcgagggcat catcggtccg gccgctgaac cgagtcgcca gccgaaccac ccagtcgcga
     4861 tagcggtagt acagggtttc gaaggctgcg gtgtcaccct ggttgatcgc ccggatcaac
     4921 tcctcgtcgc ttcggaggtc gggtggagac agggaaccca cgggaagact cctgataccc
     4981 cccagacgcc ggcaggcacg acgaccggag caattagggc tggtgccaag ctctccatgt
     5041 atccgatcat taataccccg tccaccccaa gaaagttgaa tgaaggaact tttcgggcaa
     5101 gtttgcgtct aaacgacggg ccgaaggata gaatccagtg tagggctcat aaccgcacgg
     5161 ggctgggtat gcccccatgc cgcgggtcac tcaggacatt caggagagcc cctgacaggc
     5221 gccccacaca ggtcgctcat cggccgactg tcttgcccag aaacggagga acacgtcatg
     5281 aagacctcgg tgaaatctct cggtattgtg gttccgttgc ttgccttgtt agcactcgct
     5341 gccggcggcc ccgtcccacg ggcgatggca gacggtttca tcgtggtcga tgatcgcagc
     5401 gtcgaggaga tccggcggct gtggcctcat ccacgccccg tgccgcggcc gacaccctgg
     5461 cgataccaca tgccgctgca ggtgaagaat cacctggtcg aggccgagct caccgacagc
     5521 gtcggcgtca cgcgtatcga ccagaccttc cacaacccca acaacgttca gctcgaaggc
     5581 acttacatct tcccgatggc cgacgatgtg gccgtccaga agttcagcat gatgatcggg
     5641 gacaaggagg tgcaggccga ggtcctcgac aaggacaggg cgcagcagat ctatgaggag
     5701 atcgtcagca agatgaagga tccggcgctg ctcgagtaca tcggcagccg gatgtacaga
     5761 gcccgtgtgt tcccgatccc agccaacggc gacgtgcgga tcaagctcga atactcgcag
     5821 gtcatcacgg ccacgaacgg cctggcgacc tatcgctacc cgctcaacac ggagaagtat
     5881 tcgtcggctc cgatcagtca ggttgccatt cgagcccgca tcaagacccg ccagccgttg
     5941 gccacggtgt tctgcccatc gcaccaggcg agcatcgacc ggaaggggga taacgaagcc
     6001 agcgtgggct atgaggcctc caatgtgctg ccggacaagg acttcgtgat cagctacaag
     6061 accagcaacg aggacttcgc attcgtgttg ctgactcacc ggcaggcggg tgaagaaggc
     6121 tacttcatgg cccggatcat ccccggcgaa ggcgcccggc ctcgcaaggt ggtagccaag
     6181 gacatctgct tcgtcatcga cacctcgggc agcatggccg gcaagaagat ccagcaggcc
     6241 cgtgaggctt tgcgatattg cctcgcgaat ctcaacgcgg acgaccgctt caacatcgtg
     6301 gctttttcga cggaagcccg gccttacagg gacgtgctgg ttccggccaa gccggagaac
     6361 gttgagacgg cccggcagtt tgccgccgag cttcaggctg ccggcgggac ggcgatccat
     6421 gacgctctgc tcgcggccct gaaagcccgc ggtagcagcg atgaagacaa gggcaggccg
     6481 tacatgatcg tcttcctcac cgacggcctg ccgaccatcg gcgaaaccaa cattgaccgg
     6541 atacttgaga acgtgaagaa ggccaatgcc ggcggtgttc gtctttttgt cttcggggtg
     6601 ggaaacgacg tcaataccaa gctgcttgac aagctggccg aggacaacca cggctcacgc
     6661 gactacgtcg cggaaaacga ggacctggag ctcaagctct cgaacttcta cgggacgatc
     6721 gccaatcccg tgctcagcga cctgaagttg accttcgaca gcggcctggg tgtgcatgac
     6781 ctgtatccca aggtactgcc ggatctgttc aagggtggcg aactggtcat tttcggccgt
     6841 tatgccaacc ccggcaaagg caaggtggaa ctggccggcc gttgcggcga tgaggacctg
     6901 aaacgatcgt gggaagagtc attcccgcag accgacgcgg gccaggaata cctgccgcgg
     6961 ctgtgggcaa cacgcaaggt cggctatctg ctcgatgaaa tccggctgca cggcgagaac
     7021 aaggaggttc gcgaggaagt cgttcagttg gccaagcggt tcggcatcgt cacgccgtac
     7081 acctcgatgc tgatcatgga ggaagacgag ggtcggctcg cgcggggcga gagtgatcga
     7141 attgaaggcc tgacgctgca ccgcaggttg gtgaaggcac ggccacgacc gccggcgccg
     7201 gcgagacccc gggactccgg ccgactgttc agtatggacg cgggggacga ggcagtggcg
     7261 atgtccaagg aggcgaagcg gatggccgtt gcggaaaggc ctcatgagat ggacagctat
     7321 ttcgggggct acgctcgtgg ttcggttggc agagagcccg ccgccggcgg agtcgcgagt
     7381 ttgggcgatg ctgcccggta tgggtatgcc ccagccagtc aaccggccgg ccaagaagat
     7441 gagcaggtga tgcgttacgt cgggtcgcgc accttctacc gcgacggcga gaagtgggtt
     7501 gacagccgct acaagaagga gcttcagacg acgacggtca aggccttcag caaggagtac
     7561 ttcgaactgg tcgccgctca tccggacctg ggcaagtgct ttgccttggg cagcaaggtc
     7621 atcgtggtcc ttggtgacaa ggcctacgag acaaccgagt agccgctgcc tctggattcc
     7681 tatctggagc tgccgacccc caatgttcac gcgccggccc cagattccca tctggagcct
     7741 cggtggcgag gatttccatc ctcataatcg ccgaatgccg aagggattcg cnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaggattcc gggccgagcg gagccgctgg
     7921 ccccagattt tccgcccgct ggccccagat tcccatctgg ggcctcggtg gcgaggattt
     7981 ccatcctcat aatcgccgaa tgccgaaggg attcgcgaag tgaaacggcc cggaaaggat
     8041 tccgggccga gcggagccgc tggccccaga ttttccgccc gctggccccg gattcccatc
     8101 tggggcctcc gtggcgagga ttcccatcct cataatcgcc gaatgccgaa gggattcggg
     8161 aagagaaacg gcccggaaag gattccgggc cgagcggatt cttagccgct ggccccagat
     8221 tttccgcccg ctggccccgg attcccatct ggggcctccg tctactgctc cagtagcggg
     8281 ccgtataccg ccggccggcg gtggtggcgg ccgtagatgc cgttctcgcg gtgcttcttg
     8341 agagcatcca gatcgaacgt cgcgtcaaga atctcctcgt cgcgcgtgcc gagccggatc
     8401 acctcgccgc tcggggcgat gatcatgctg tgccccttga acacggggcc ggcccagttg
     8461 gccatcgcga tatacacctc gttttcgtag gcccgcacgc gaagttggtc gcggtgaatc
     8521 ggtatctcgc aggtgcagcc ggttgcctgc gggatcgcga tgatgtccgc ccccaacacg
     8581 gtcaggcagc gcgcggcctc aggcacctcg cggtcgaagc agatcatgat gcccaggttg
     8641 aacgaaacac catcaatctc gcagggccac acctggaagc gatcgccgtt ggtccagccc
     8701 atgtggctct cgtacttgtt gatgtgcacc ttgcgataag tgccgaggat ctcgcctttc
     8761 ggtgaaagca gaacggcggc gttgtagtac ttgtcgccgg atcgctcgtg caggccccag
     8821 acgatgtaaa ccttgagctc ggccgccagt cgggcaaagt gattcaccga agggccgggg
     8881 accggctcgg ccagttcgcg cgactgagcg agggtcaaac ccttgtcgga cggcgacgtg
     8941 tagccggtga gcgtggtctc cggcaggatg acaagctgag cacggtgctt ttgcaccgcc
     9001 tcgcgaacca gccggtcggc tcgttcgacg ttggccttgg gatcgccggt cgccggcagc
     9061 atctggatgg cggcgacgcg cacctggcgt ccgccgacct tcttcccgtc ctctgacgcc
     9121 ttctcagaca ccggccgcgt gtcatcatga ttcaaactca ctgtaggtcc tccacgccgc
     9181 gtatcgccgc cgcagccgca cgcaagagcg acaaacacca acgccgtttg tgagacatgc
     9241 cgcaaacatg cggcacgccg gcagtgattc ttgttcatac atggtcctct cgtcatcgat
     9301 ctgaccagga tcaggccgca agccacagat agcctttgcc cccgcagaat caggctcggt
     9361 catcgcctcg acgcctgccg ttatgcgatc accgaacata cttgatggcg gcgaaaccgc
     9421 caaccagcag gatcacgaac acaacgcaga gcagattgaa gtagcgatcg atgaatcgct
     9481 tcatcggcgg accgaagaaa tacaggagcg ccgagacggc gaagaaccgc gcgccgcggc
     9541 ccacaatcga gacgatcacg aaccccagca gattcatatg gaacgcgccg gaagcaatcg
     9601 taaacacctt atagggaatc ggcgtcagag aggcgatgaa tacgatccag tagtcgtact
     9661 gctgatacca ctgcgtcacc tgctgataat acgactcggc atgatagaag gcgatgatcc
     9721 gcgtcccgat cgtgtccatg aaggcaagtc cgatcccgta gccggcgatg cctccgattg
     9781 cgcttgcgag cgtgcaggtc atcgcgaagc ggaaccaccg ggcgcgtgat ccgagcacga
     9841 gggcgatgag cagcacgtcg ggcggtaccg ggaagaagct tgactcagca aaagccagca
     9901 aaaacagagc aattgcgccg tacggcgtgt cggcccaatg gagcacccaa tcgtacattc
     9961 ggcggtgaag atgccaacga ggtatggccg gcttagccgt gggcggagag accgcggtag
    10021 actggccgtt attggtcatg agtcgtgcgt cctctgtgtt ggcaggcact ctaaccggcc
    10081 aatatctggt ttgacaaaca tgctctccga cctccgggtg acggcttaac gccaggcgcg
    10141 gtgtgttgtc cgctgatttg ggccgttcgg gcctcagttg cgggaggcgc ctgcggggga
    10201 ccggttgtcg cgggggtgcc cgcacgaggg gggcgaattg aagcaggagt gtaagttggg
    10261 tttgtttggc aaatcgatcg gtgagccagc ggcggccgac tgccagatgc aggaccgtga
    10321 gcacgttgtt cctctccatg cctttgaaaa acaagaagtt ggcaatcagc cgggtttgga
    10381 tcattggcat tgtcatgccg gttgttctct tcccatttca cgagcgggtc atcaattcaa
    10441 ctgatacgaa cgagcaggcg aacaagccct cttttttcgg cgtcggtggc ggtttctccg
    10501 cgttcataac acgctgagtg ctccggaggg agaggtggcc agcggagacc ggccgggcgg
    10561 tacatgaggg tggcgggcca aaaggccgga aggacgatca aggtccaggt attggccgac
    10621 ggtatcggct tggcgagaac ccgatcggca cgggtggctt ggctgccgcc gtgtgctggg
    10681 ctggagagcg ccggcgggga aggcatcggc ggcttcgggc gggatgccac gcacgggccc
    10741 tgggccattt ctgccgctta tcgatcgcgg ggcaccggat aaaaatccgt gcgaattaaa
    10801 tgccgggcaa cttaatcgcc ccctggtcgt gaccgataac gaacgttgga gcaggagacg
    10861 gatggggttg catggtactc atcgcggggt ccaccaagag gtggccaaaa cctctcgatc
    10921 cggctttgcg gcccggtctg agagctgggg tccaacgtcg caaggcgagt aacggccatg
    10981 tccataccac tggtcaacct caagcggttg cacgacaagt tgtacaccga gattcgaacg
    11041 gccatcaacc gagtgctggc ccaaggtgat ttcatcacgg gctcggacct ggcggcgttc
    11101 gaggaagagt tcgccgccta ctgcgagacg aagtactgtg tgggtgtggg cagcgggctg
    11161 gacgccctga cgttggcgat gaggggtttg gggatcggtc cgggcgacga ggtgatcacc
    11221 acctcgaaca cgttcatcgc aaccgcgctg tcgatctacc atgccggcgc cacgccggtc
    11281 ctggtggacc atgatccgga gacatacaac ctcgatccgc ggcggatcgc ggcggcgatc
    11341 accccgcgaa ccaaggcgat cgtgccggtg catctatacg gtcagccggc cgagatggac
    11401 atgattcagg ccatcgccga cgagcacggt ctgttggtca ttgaggacgc ggcgcaggca
    11461 cacggcgcac ggtacaaggg gcgtcgggcg gggagcatgg ggcgggccgg gtgtttcagt
    11521 ttctatccgg gcaagaatct gggagcgatg ggcgacggcg gtgccgtcgt gaccaacgat
    11581 gaggatctgg ccgcgtggct catggcggct cgcaattatg gttcgacggt gaagtattac
    11641 cacaccatgc cgggttacaa ctgccggctg gacagcatgc aggcggcgat tctacgggtg
    11701 aaactgcgct acctggacga atggaatgcg acgcggcggc aactggccgc ccgttaccgc
    11761 gagaagctgg ccggggccaa catttcgccg cccgtcgaac gtcctcacgt cgaccacatc
    11821 tatcatctct tcgtcgtgcg atgcaatcag cgggaccagg ttctgaagca tctgaattca
    11881 aaaggcatcg gggcggggat tcactatccc gtgcccattc accgacaagt cggcctgagc
    11941 acgcggtgca tcgtcccgcg gccgctggtt tacaccgatc ggtactgcga ccaattgctg
    12001 tcgctgccga tgtgtccatg cctgaccgaa gcagatatgg acatcgtggt gcgcgagctg
    12061 agtgcggtca cggcgacggc gccgacggtc acggcatagt ccaccccggc accgcacacg
    12121 gcaccgccgg caaatgaaga acgacgaagg cgggcgaatg gtccgccggg gcggccccta
    12181 ccgccgaaaa catgaaatgt tcaaaccccc ggtgcacaga atcctccggc ctgcaagccc
    12241 gttgagaggg caggcacgtc ggatctgacg ccaagtcaaa cgcaggtcgg tcgaagaacc
    12301 tgcccgagcg gatggaagcc ctgtgcccgg caaccggcaa gcctctcttc aacgagccac
    12361 agtgacgttc acggatgatg tggcggcatt tcctgaggcg tcagtggcac ggacggtgat
    12421 ggtgtgcgat ccgagcgcca cacgtgggtc ggcggtgttc caattgtgag ccacggtcgc
    12481 gcccgagtcc gacggttcca gcgagaggac actgttaccg tcgatgagga tttcaatccg
    12541 cgacaaggcc cggtcgtcgg ttgctcttgc gacgatgctt accatgcctc ggacggtggt
    12601 ttgtgaacgg ggggaggtaa tgtcgacgat cggaggaata ccatcgggag cggacgtgat
    12661 ggtgacgttg atggccgctt cgctcacgtt gccggcaagg tcgaccgctt tcacccgcag
    12721 ctcatgcact ccggtcgcat actttccggg atcgagcacg aagccgtaag gccagacgga
    12781 atcggaagcc ttccgggttt gatcgacgta aagcacgatt tcggcgattc ccaaggcatc
    12841 accggcctcg acctgaagaa cggttggcga cgagacggcc gtgccaccgg cgggcgagac
    12901 gatagcaatg gtgggcgggg tcgtatcctg ctgttcgacg atccggacgg ccagttccac
    12961 ggcggttgcc gcatcgacgc atccgaaggc gaaggtctca tcccagccgg tggggccaag
    13021 gtcacgagcg gttgccagga ggatgtcgcg taccgtcgcc ggccgaagat tgggattcac
    13081 cgaccagatg agggcggcaa tgcccgaggc gatgggggcg gcaaaggacg ttccggtcca
    13141 agccgcgtag gcgttgccca gagttgtggt gtagatgctc acaccggggg cgaccagatc
    13201 gacgaaatcg ccggaggtgg agaaggaggc cagcttgtcg aactgatcga tcgcgccgac
    13261 aaagagcacc tcgtccgatc cgaccgcctc tacccggcca ccgccgttac ccgcggcgat
    13321 cacggtcagg cagcccgaga cccaagccat gcgggcctgt cgaagcacga tgtcgttttc
    13381 gtgcaggggg gcgaatgaga tgttgatcac tttggcgccg cgattgacag ccaaggcgat
    13441 gccggcggcg atggaccagc tatcagcctt gttgtcgggg cccgtgacac gaatcggcag
    13501 gatgggacaa cccggggcaa cggaagccgc gccgtcggcg ttgttgcttt gagccccaat
    13561 aatcccggcg acggccgtgc cgtggccgga agtgtccgcg tagctcaagc ctgtgtcgaa
    13621 ggtgttggcg ccggttctga gcttgtctgc gaggtcagga tgtgaggcat cgacaccggt
    13681 gtccagaacg gcgaccgtaa tgttgccact tccggcggtc agtgtccaag cctgaggaac
    13741 gtgcaccgtc tgaaggaacc actggatggt gtagtcggga tcgttgggcg tgatctcgac
    13801 ctggataagc cggttgttca agacctcctc gaccagggga gagccacgaa gcgatcgggc
    13861 caattgttcg cgattgcggg ggtcaacgcc gacgaggtag gcggacaggc cctgtagctg
    13921 gccgttgacg gcggcgtcat ggccgttcag gagattcacg agatcgccct gtgtggcacc
    13981 cggtctgagg gatacgatga gttgatcggg aacgatatcg gaattggctg aggggaattg
    14041 acagcctggt gccatgacaa aggccgcgtc cacgaccaag gcaatgaagg ccaatgtcgc
    14101 gcggcttgat cgtcgcgtgc cattcctgcg taaggcctca tcgtcagggg gttgtagaat
    14161 catggttttt ctggaacagc ccacaggtcg gcaggagtgc ggttcctggc gcaggagagc
    14221 gcgacgaatg agcctgcttg cgtttccgtt gcctgacagc ccatgccgcc atcgatcctg
    14281 ctgcccggac gccgtccctg gccatccgag atgagggctt gcctggtaca catcggatga
    14341 ctcggtcaac cgctgtggtc catattccag aaagccgttg gatgtcacgt ccacggccgg
    14401 agcgcttcgg cggagtcgaa caaggactcg acagaacctg atacttggat accgggaagc
    14461 gtccggatct cgttcagcac agggagtgat acctgtaagt catcgagccg caaggtgtta
    14521 cgaatgcgga tcaagcgaac ctcgtcgggg cgggacaccg ggcaagcctg cagggccatt
    14581 cggaccgctt cacggtccgt gtcggcgatg atggggacct tagctcgctc caggaaggtg
    14641 gtggtcagga cattggcgta ggttgaagtg aaatcgatct tgtcgaacag tcggcgggta
    14701 ataatgtcag ccaggcccat gccaatggca ttaccgtgtg aggccgagga gagatcgtga
    14761 atggtgatga cgcggatgtc aggggacttg ggctctggct gaccgcggat cttcagccgc
    14821 ccgatgacat tgggatccat gccgaggccg ctgatgtcct tgccgatctg gtcaacgatc
    14881 agcacgtcga ggcgatcaac gggcaaagcg ggcatgttag cgcgggcgat gtcgagcagg
    14941 gccggctctt cggcgggaat gtcgggtgcg cgaatggcgc gaacgagcat cggttcgtca
    15001 taggcattct cgacgatcgc gaggcccagg aggatattgc catgggtgag aatccggccc
    15061 gcgacgcgcg gcatcatctc gcggaggccg tcgacgccga ggcgatgaag agcctgggct
    15121 tgagcatgtt tgcccagccc gacggctatc atcttcatga ggccgctctc gtatcgaccc
    15181 cgaaaagaag tgtgcatctt gatgcgattg atgaggatcg tgttgtcggc ggaggcggcc
    15241 tggcgatcaa gaaagacctt cgtgggcaga tcgccctggg gcagttcgac gacctccatg
    15301 gatgatcgca ccggacaccc gacgttctcc tcggttacac cgtaagctgc caataccgcg
    15361 cgttgcccct cggcggtggc gccgccgtgg cttcccatgg ccggcacgat gaagggctcg
    15421 gccccgcatc ggcgaaccca ctggacgacc gcccgtacga atcggtccag ttcagcaatg
    15481 cctcggctgc ccaccgcaat cgcaatccgc gagcccggtt gaagattgac gccgcagtga
    15541 atgagttctt cggatacgga agcttcgata tccgtgagcc gatcagccgg aaaggtccgc
    15601 cggacccggg tgatggggat gaaatccatt gtcagccagc ctcctgggca aaacgcatgt
    15661 tgcggtctga gctttcggtt ggaccgcgcg cgtcgacggg gattctatcc tcgctgacgg
    15721 gcgtcgcacg agcggatcag gagaggtcgt tttcactgtt gcctgggggg ttgctgttgg
    15781 tcgtggcaag atggtgaggg caagcctggt aaatcagaac atatcagaca agatcactga
    15841 tatacacaag gctgatatgc ggttggggaa ggtcgcgccg tacgggtgta aacatgacgg
    15901 gtgatcgcga cggttcgcaa gcaagtcttc ctgtgagcat ccgtgcctgg gttgctgcca
    15961 tcctcggtgc tgagctgttg gaagaggact tggcggtttg gcgagcaggc actgagagac
    16021 tgattgccca gcccaacgct cagcattttc cttttgaaca ccgacgcgtt cccggaacac
    16081 tcttgtgact acggcatcga accagaccac ctgggtcttg ctacggcgaa ggcgatccgg
    16141 gcaagtttcc gatgcagtag caggctgaat gttcgggtca gacaacccgc cgcgaggggc
    16201 gttgttcaaa cggatatctt gatcggccca gcggatcggc ggaatcaagg ggtggtggag
    16261 acccgcgcgt tcgaactgta agatagctca tccgggaagc cgcagaggcc tggcggcgtc
    16321 gggtctcagt gaaatcccgc agggtattgg cgcagcgggg ttgggcatgc gcgagtggcg
    16381 gaattggcag acgcgcagga tttaggatcc tgtggggaaa cccgtggggg ttcgatcccc
    16441 ccctcgcgca gttgtttacg cgggctcgaa cggtttgcga gatcgagtgg tcggcaggcg
    16501 cgggcggtac ggcgaaggat tcgttcgatt cgagagattc cagccgaacg gtccgcgggc
    16561 gcggaccctg cgatgggcaa ccgcatcatg ctcgcctccc ccgcccgaac ggtccgcggg
    16621 cgcggaccct gccctgctca gatcggcttg cacgcacagg gctttgcttc ccgcgatgag
    16681 catgaagaaa ggccaaaccg ccggcatcgc gaggggcgaa gccggctgta aaactaggct
    16741 gttgctggat ttagtggggg aaccgcctct gtcacgagcg ggagtcgcag cgattcaccc
    16801 gttccgccgg accacaccga ggccgattcg ccatcggcgc gatcgtgatg atcgccaagt
    16861 tcactaaaac ctgtaaattg tcttgacgca ccggggaagg gctgtagaat accggcacgc
    16921 gacctggagg aagcgccagt ctagggtttc acagcatcat cgcgggagag agaagctccg
    16981 cgaagtacag ggattttgaa gggagagaga atgatgaaca ctttgatctc gcgcgtatcg
    17041 gttcttgtgt cggccggctt tctggcgggc acggcagcgg cggtaagcat cgagaccgtg
    17101 ccggtgggca atgttgggaa cgccccggat cgtcgctacg gcggctacgg ttcggtcggt
    17161 tacacgtaca acatcggtaa atacgaggtg acggcgggtc agtacacgga gttccttaac
    17221 gcggtggcaa agactgacac gtacgggctg tacaacccga acatgtggtc gagcagttac
    17281 gggtgcaaga tagagcggag cggctcttcg ggcagctaca cctatagcgt tgctcaggac
    17341 tgggccaatc ggccggtgaa ctatgtgagt tggggcgacg cggcgcggtt cgcgaactgg
    17401 cttcacaacg gccagccgac ggggccgcag ggcctgagca ccacggagga cggggcttac
    17461 tacctgaacg gcgcgacaag cagcgatgcc ttgctggccg tgacgcgcaa gccggactgg
    17521 aagtgggcga tcccgacgga agacgagtgg tacaaggcgg cgtatcacaa gaacgacggc
    17581 gcgacgggga actacttcga ctacccgacc agcagtgaca gcgcgccgag caaccaactg
    17641 agcgatcctg atcccgggaa caacgcgacg tactacaaca acggctggac gatcggcaag
    17701 ccgtactacc gtgcggaggt gggcgctcac gagaacagcg acagtccgta cggtacgttc
    17761 gatcagggcg gcaacgtgtg ggagtggaac gaagctgtta ttggggcttc tcgcggcttg
    17821 cggggcgggt cgttctacag tttcgacccc gacctgctcg cgtcggctcg ctactccggc
    17881 gaccccttgt ccgaggagtg cctcgatggg ttccgcgtcg tcgaggttcc cgagcctgcc
    17941 atcatgatga tgatggccct agcggacctt tccgacttat gaccgcgcat tgcatgcggt
    18001 tgaagcccaa tc
//
LOCUS       DAOTPW010000112        17683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3201_length_17683_cov_5.318811, whole genome shotgun sequence.
ACCESSION   DAOTPW010000112 DAOTPW010000000
VERSION     DAOTPW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17683
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3201_length_17683_cov_5.318811"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="P5316_11570"
     CDS             <1..393
                     /locus_tag="P5316_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRR85636.1"
                     /translation="RINPTAAETIRKLLIGVVTEGTGKPANIPKWQLLGKTGTAQIPK
                     IGGGHYEPDAYLGSFVAAAPASDPRVAVLVMIRKPKRSIGYYGATVALPAVKTIMEGV
                     LPYLNVPPDKEEPQKHANLARDSQTKYN"
     gene            778..2346
                     /locus_tag="P5316_11575"
     CDS             778..2346
                     /locus_tag="P5316_11575"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRR85637.1"
                     /translation="MPNHGLRWSQLIGETGLIERWDGPCDPVVTSVLEDSRQVTPGAC
                     FVAATGLRADGHAFVEAAVEKGAVAVVSERPVALPPGVAGLVVGSSRGVAGRLAATLY
                     GLDKAQRDGRLRVIGITGTNGKSTFCFMMRSILQVAQCPTALLGTIEYDLLARKVTAS
                     MTTPPATAIMSYLAEAFEAGATHAVMEASSHALDQDRCAGIRFSVGAFSNLTGDHLDY
                     HRDMESYLRAKKKLFDGLDSSAAAVVNMDDPHGDAVAADCRGRVIRYGVQLNASRPDG
                     RVPDVCAIIKEISASGTRFVLVLRGTVTASGREESCELCSPLIGNHNVQNSLAAAGSA
                     IALGVPLVTVVAGLEAAKCIPGRLQRVETPAGRARGDRGFTVLVDYAHTDDALKNVLS
                     ALKPLVQRSGGRLIVMFGCGGDRDRTKRPRMARVAAEWADRIVVTSDNPRTEEPMSII
                     EEIMTGFDPSDVSRVCVEADRRKAIEAAIGFADRGDIVLLAGKGHETYQEIGRERFPF
                     DDSAVAAEVLSGLR"
     gene            2433..3971
                     /gene="murF"
                     /locus_tag="P5316_11580"
     CDS             2433..3971
                     /gene="murF"
                     /locus_tag="P5316_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRR85638.1"
                     /translation="MRPLTFAEGIEALEGTCDRPVSAGSFSRVCIDSREVQPGDLFIA
                     IPGERFDGHDFVEQAIAGGATAAVVRNDFKLPSSRVGPSRVTDPKPEALLIRVDDTVK
                     ALGRLGRYYRRSVVGGSLNVIAVTGSNGKTTTKTMIAHVLSGRWQGRASVKSFNNSIG
                     VPLTLLSVEPSDAFVVCEVGTNAPGEIAALAKLVEPEIAVVTGVSEAHLEGLGSLGQI
                     AEEKLSLLHYLRPEGLAVVNADCEVLRKTLERDRRLAGLKCVTFGEWKGADIRMTSLQ
                     VSMAKKADRRVSQTLPPVHLEFTVNDRFKYELQVPGRHNVLNALAAIGVARRLGVDHE
                     EIAARLATFSLPPMRLEYQRVGDLTLINDAYNANPASLAAAVDVLVSLPTEGRKVLIV
                     GDMRELGQASDELHRQAAEKIAGSGVNMVISIGEKSRLVSRTIEAVSGGAVETHAYSS
                     AVSAKRRLVSYLNKNDTILMKGSRLLALEKLVPVIREWAVLPEGKTPVGAGSRKKLRS
                     SAAL"
     gene            4050..5186
                     /gene="mraY"
                     /locus_tag="P5316_11585"
     CDS             4050..5186
                     /gene="mraY"
                     /locus_tag="P5316_11585"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRR85639.1"
                     /translation="MLYHLFDALYGHPAGPYAYKDPLFRGTCALLLGFFLVWFLGPWF
                     IRQLRRYSIGDQAEFDHAQLNLDNADKRNTPTMGGLLIMAVILCNVLLLADLNNYYIT
                     MGLFCMVWLTALGSVDDWLKLTIKRRTGTRDGLKMHEKLLFQIALAVLLGFYIYRHGE
                     YNATTISGDQIQTFRILNVPFYKTGWELNQVAFFIITILVVTGTSNAVNLTDGMDGLA
                     SGCMVLCSFVFMILAYIGGDRELANSLLFPHIPHTAELAVLCGAMMGACMGFLWHNCY
                     PAKVFMGDTGSLPLGGMIGYVAICTRQELMLFIVGGVFVIEAVSVIMQVGYFKMSGGK
                     RIFRVAPIHHHFHLGGWTETQTVTRFWLLAIVFAGIALATVKIR"
     gene            5297..6172
                     /locus_tag="P5316_11590"
     CDS             5297..6172
                     /locus_tag="P5316_11590"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRR85640.1"
                     /translation="MLPCEMLRIRHSDHYEAFARLWLPRSPRGAVLYLHGIQSHGLWF
                     EASAAHLAEAGFAVLLPDRRGSGRNEVERGHASSARLLLRDVADGLDELHVRTGLPRF
                     HLLGVSWGGKLAAAFWNHAPARVATLTLVAPGLFPRVDVPLTQKIRIGLASVVARRKL
                     VDIPLDEPELFTANPARQAFIRSDKLKLSRATVAFMLASRRLDARVARLARHSPGCPL
                     KVFLAGRDRIIDNEKTKAFVRSLPWPTRGVVEYPEAHHTIEFEPDPRPFLDDLVAWMS
                     SQADVKSGEVLAAGP"
     gene            6197..6955
                     /locus_tag="P5316_11595"
     CDS             6197..6955
                     /locus_tag="P5316_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR85641.1"
                     /translation="MITYSLQSGSNGNAIYVEAGDVRLLFDAGVSGKTAEGRMRVRGH
                     EIRDATALIISHDHADHMRCAGIYQRKFGLPIYMSARTHRAYRENLGHLKDVRYFEVG
                     QPLRFNGVVVYTVPTPHDAADGAVFVVEHAGRRLGILTDLGHSFAALRRLLPTLDAAY
                     LESNYDPHLLATGPYPLSLQARIRGSGGHLSNQEAAALVRESAKRLRWVVLSHLSEQN
                     NRPELALEAHRREVGPRFPLYVASRYDLGPLMSL"
     gene            7152..8174
                     /locus_tag="P5316_11600"
     CDS             7152..8174
                     /locus_tag="P5316_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85642.1"
                     /translation="MRRAKAPVAAFTLIEVLVVVAIIALLIAILMPSIRAAQDAARAS
                     VCGNNLKQCLTALQINLAEITARRETERISTNYGWAAIVYRYSNRQAGIVTCPSDPEP
                     WPIPALYVRILGERSELLSGDALYNRPKFIAETRYELDIQDVVAGRGFGNDAWNTNDI
                     DLLLEYEAPKKGDNAPVKVKNKESALGFDVLDYRKRTVWANVSGPISTPVGMPLLWMS
                     YGANAAAGLKGVRGNPILLVELPKPGVFPEKYGSFPADDMKKSLRFRHGEKAPPSAGL
                     GGWNYKTDVVNGKAGGEGYVPRIRMNVGFYDGHVERMHHLQMIKSPPGNLWLGNRAPG
                     YVPEFW"
     gene            9107..10336
                     /locus_tag="P5316_11605"
     CDS             9107..10336
                     /locus_tag="P5316_11605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR85643.1"
                     /translation="MRCSSVLAIAAIVCVVAMVTFGQIAPPGSQPASKAATAPADEVA
                     VVVNDRPIMESEIDRVIAQGRKITPEELAQKRQQIKPQQMKFLFDYIIGNELIVQAAG
                     REGIVLTDQEWKEEFRKTFDKWVQANGWSQEQAAQEIRTRFDMDLAELEEEMPKSPGF
                     RAPILQEKIMQKKFADELRVTDEDVKAYYEQNKANRYTPKEEEVRASHILFATRDAKT
                     RQPMSEEAKQEQRKKAEQILTEAKKPGADFAALAREHSSCPTGKRSGGDLDYFPRTRM
                     VPAFSEAAFAMKVGEISDIVETEFGYHIIKVTGRVEAGETKPFDTVKDQIRTQLEQQK
                     LRTVVDRYKEELKNKAKIVYPPGKEPASPPPVRPVTTRPTTQSTRAAMRPRASSTTRP
                     GDRASVSLPRPQTAAAE"
     gene            complement(10415..12373)
                     /locus_tag="P5316_11610"
     CDS             complement(10415..12373)
                     /locus_tag="P5316_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRR85644.1"
                     /translation="MWFSLFATVLILAVTFYQGLQGMFSALINCVLTILAAALAFAYY
                     EDLYQSQLITYQPDHGRAIALMAIFIVSLLVMRTVVDLLIKGNQTFPIYLDRAGGGIF
                     GFITAMIIVGMLAIGFQMLPFDPTLLGFSRYSLVDASSNKEVVTETKSSATSKGDQNT
                     NVVYRNEIDWSNVKAIRHNLWLNPDGLTVALMSHLSRFALRGRNSFAEINPDFLDYIH
                     HIRDGLGREPLAAVPPNAAIIKQYEYLRKDEPIYKRAKVRDENSVVTLKYEPVNRKPQ
                     EGQRWLCVTVTINEKTDVCQDRANLNFTSSQVRLLARDRNNGPVKVYSLAAINESDPA
                     NAHRLIQVFPCQDLQYNRAGGSPEMRLLFEVPDNPDFRPLAIQYKMNAWAEILPSHDQ
                     TKTRAGTVSDTSLTERPSDRDRPRESRDDVSTSRQPRPTRDDTDASSAATERRPSPPR
                     PDPNPRDRVHGVNLASQEPFFSERLPFAAPLTDYTEGMEFERSDGKLRSARGFTVKLN
                     ADWEPVAGSKPPLESFDVPADMRLLHVNVERLQPGSTLGKARGTATELTRDIYVTDGN
                     GKQYMPVGKYAIATIRGETIFELTYLDETARGFARMPGLEHIRFEDLKGKYAYVFLFH
                     LPPGAKPMKFHTGRTAIALDQYNLVAPE"
     gene            complement(12380..14215)
                     /locus_tag="P5316_11615"
     CDS             complement(12380..14215)
                     /locus_tag="P5316_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRR85645.1"
                     /translation="MSVSKRRFTAVSLSAGLVLTVPSSSLAASALAALSEPGGYMSPW
                     RIALVLVCLLPWMAFCQWVDKDTQYVRRMRRDTWNGVILGGGTVGLVVWILMPWNSVG
                     LFAAGFGLWFLATVGTCATYVVLRNGLVDANARVFTVRHIKSWLGGLGKKTEGKATAF
                     ERVRMTRHNGEKVPVPTDPTQTDAFEATQNLLFDALWRRATEVEMLISPSDVRLAYRI
                     DGVATPRTDLLTRDAALQALNLIKVVAGLDVEERRRPQQGEMSAVITGTNAQPTEIEV
                     RTSGTTQYEKLSLRIVGEENRLRIGDLGMDEKQLAAMEEIIRKPSGLVIVSGPHGSGV
                     TSTLYAALRNHDAFMQNLLTLEREPLMDLENITQHIYDSTKHEASYARQLQTVLRREP
                     DVVMVSDCPDRETAHLAAKAACDGKKIYMGIQARDTFDALKKLMSLAGDLDTVAKALV
                     ALTSQRLVRKLCIACRQPYKPDPNLLKKANLPADKIDHFYRPPPGGLVDAKGNPILCT
                     NCQGSGYFGRTGVFELLEIDDGLRDLITKGQPVSAIRAMARKNGMLYLQEVGLQKVMN
                     GVTSMTELLRVMREEEAGGNSQPPPAASVSKPKNPKSDGAGKERG"
     gene            complement(14344..15609)
                     /locus_tag="P5316_11620"
     CDS             complement(14344..15609)
                     /locus_tag="P5316_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRR85646.1"
                     /translation="MAEEDIYRRINERTHVGDEPVEAAPALEEIEPSEPPAPAPVKSA
                     APEKNRTVAASAKREPKIHKYLKYVVDIQASDIHFKSNARVHIRIKGDLKPIKGEPLS
                     PQEVENLWFEIMNEHQRKQLAEKGASDFAYQLGDSDRFRVNIFRQRGQLSVAARRVNK
                     NILNFDQLHLPKSIYKVTEFHQGLVLLAGITGSGKSTTIAAALDYINAHRACHIVTIE
                     DPIEYLFTDKKALINQREVYVDVYSFADALKYLMREDPDVVLIGEMRDEETFTAALNA
                     AETGHLVFGTVHASSSSQTINRILDLIPEGSRDLVRQTLVFNLQAIICQKLLPSIKPG
                     LARVPAVEIMFATPTVRKLIDEKRDDELGKVIRASQSEGMLDMNECLKRLVEQEYIET
                     AVAYAASPNPQELKMRLKGISAGAGSILG"
     gene            complement(15645..16412)
                     /gene="hisF"
                     /locus_tag="P5316_11625"
     CDS             complement(15645..16412)
                     /gene="hisF"
                     /locus_tag="P5316_11625"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRR85647.1"
                     /translation="MLAKRIIPCLDVHAGRVVKGVNFINLRDAGDPVEIARGYEEAGA
                     DELVFLDITASHEGRDIMLEVVRRVAEECFMPFTVGGGIRTIEDVTRLVQAGAEKVSI
                     NTAAVLNPNIITETARRFGRCCTVVNIDPKRVWKAGREFWDVHIHGGRTPTGLEAVAW
                     AKRVEELGAGEIVLTCMDADGTQEGYDLEITRAVAEAVSIPVVASGGAGEPKHLADAV
                     SIGKADAVLAASIFHFGTYTIRETKQYMADRGIPVRL"
     gene            complement(16972..>17683)
                     /locus_tag="P5316_11630"
     CDS             complement(16972..>17683)
                     /locus_tag="P5316_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85648.1"
                     /translation="EYSYYWKGASAGESSWFELILLDGPFSMQYADQFQESTSANNPY
                     MIRKRVLGAGSFAWEQITHLTPADTGPAGPRPQTITPTGTVVTVVLKAGRNPGGSMES
                     LWDNVVVTQNGGPNLISNGDFENGGQAGICDRETVFQDECEFDSWRRSSFVPLECLNW
                     DPFADADQDGDVDQADFAALQACYTGPGSFTLPAECACFDRPEPLEPDGDIDQADLYK
                     FEDCASGPGIPANPACDD"
BASE COUNT         3345 a         5081 c         5617 g         3640 t
ORIGIN      
        1 agaatcaacc cgacggccgc cgagaccata cgcaagctgt tgatcggagt cgtcaccgag
       61 gggaccggga aaccggccaa tatccccaaa tggcagttgc tgggtaagac cgggacggca
      121 cagataccga aaatcggagg cggccattac gaaccggatg cctatctcgg ttccttcgtc
      181 gcggcggccc cggccagcga cccaagggtt gcggtcctgg tcatgatccg caagcccaag
      241 aggagcatag gctactacgg cgcgacggtg gcgttgccgg cggtcaagac gatcatggag
      301 ggtgttttgc cctatttaaa cgtcccgcct gacaaggagg agccccagaa acacgctaat
      361 ctggcgaggg attcccaaac gaaatacaat tgatttgtga tgtcaggcgt ttgtccggac
      421 tcgtgaagcg cgttgtccgg ggtacagacc acggccgcct catacagcgg gcaagggata
      481 agacgatttg cgcggtacgg tcagagccca ttcccgtcgg tgccaaggtt tatttggccc
      541 tggtttcgcg atcgccggcg gttgcccccg gttcgcatgc ctgccgcgcg ggtcacgttc
      601 ggcgagtaag ggatccgtcc tttgcacgga ggctgtccgt caactggaat agaaccttac
      661 ggccagacat ctgtgcggct tgcgcgcatg tttctggatc gcagagcgct tgggttgcgg
      721 cctgcaaaaa ggcggtccgc cttaggacat actggaacga ggaactgacg gaagaagatg
      781 cccaatcatg gattgagatg gagccaactg atcggtgaga ccggcctgat cgaaagatgg
      841 gacggtccat gcgaccctgt ggtcacgtcg gtcctggaag actcccgaca ggtgacgcct
      901 ggagcctgtt ttgtcgcggc caccggcctg cgcgccgatg gtcacgcgtt tgtggaggcg
      961 gcggtcgaaa agggcgccgt ggcggtggtc agcgagagac cagtcgcttt accgcctggg
     1021 gtggcgggtc tggtggtagg cagcagccgc ggagtggccg gccgcctggc tgcgacgctc
     1081 tatggtttgg acaaggctca acgggacggc cggctccggg tcattgggat caccggcacc
     1141 aacggtaaga gcactttctg tttcatgatg cggtccattc ttcaggtggc tcaatgcccg
     1201 accgcgctat tgggtaccat cgaatacgat ttgctggctc gcaaggtgac ggcctcgatg
     1261 accaccccgc cggcaacggc aatcatgagt tacctggcgg aggcttttga ggccggtgcg
     1321 acccatgcgg tcatggaagc gtcaagccac gcgttggacc aggatcggtg tgcggggatt
     1381 cggttttccg tcggggcgtt cagcaacctg accggtgacc acttggatta ccacagggat
     1441 atggagtcat acctgcgggc caagaagaaa ctgtttgacg gtttggattc cagcgcggct
     1501 gcggtggtca acatggatga tcctcacggc gacgccgtcg cagcggattg ccgcgggcgc
     1561 gtgatccgtt acggtgtgca gttgaacgcc agtcgccctg acggccgggt gccggatgtt
     1621 tgtgccatca tcaaagagat cagtgcctcg gggacaaggt ttgttctcgt actccgcggg
     1681 acggtcaccg cctcgggacg cgaggaatcc tgcgaattgt gctcaccgct gatcggcaat
     1741 cacaatgttc agaacagcct cgcggcggcg ggttcggcga tcgcgctggg ggtgccgctg
     1801 gtgaccgtgg tcgccgggct ggaggcggcc aagtgcatcc cgggtcggtt gcagcgcgtg
     1861 gaaacgcccg ccggaagggc tcgcggagac cggggtttca ccgtgttggt cgactacgcc
     1921 cataccgatg atgccctgaa gaacgtgcta tcagccctca agcctctggt gcagcgcagt
     1981 ggtggcaggc tgatcgtgat gtttggatgc gggggcgacc gcgaccgcac gaagcgcccg
     2041 agaatggcac gagtggctgc cgagtgggca gaccgcatcg tggtgaccag cgacaatccg
     2101 cggaccgagg aaccgatgag catcattgag gaaatcatga cgggtttcga tccgtccgac
     2161 gtttcccgag tttgcgtgga agccgatcga cggaaggcta tcgaagcggc gattggcttc
     2221 gcggacaggg gggatattgt tctgctggcc ggcaagggac acgagactta ccaggaaatc
     2281 ggccgggagc gttttccttt cgatgactcg gccgttgcgg cagaagtcct gtcggggttg
     2341 aggtgaacag ccgatgaaca ccgggagggt ggaaacgatc aatcgggggc tgggggcgag
     2401 agcgatccga acccagagaa gggcatcagg gcatgagacc tttgacgttt gccgagggga
     2461 ttgaagcact ggaaggaacc tgcgatcggc cggtatcagc cggtagcttc tcgcgcgtct
     2521 gcatcgactc gcgggaggtc caacccggcg atcttttcat cgcgattccc ggagaacgct
     2581 tcgacgggca tgatttcgtg gagcaggcga tagccggcgg ggcgacggcg gcggtcgttc
     2641 ggaacgattt caagctcccc tcttcacgag tgggccccag ccgggtgacc gacccgaagc
     2701 cggaagctct cctgatccgc gtggacgata cggtaaaggc ccttgggcgc ctgggccgtt
     2761 attatcgccg ctcggtggtc ggcggatcgc tgaacgtgat cgcagtcacc ggcagcaacg
     2821 gcaagaccac gaccaagacg atgatcgccc atgtgctttc cggacgctgg cagggccggg
     2881 cctccgtcaa gagctttaac aattccatcg gagtcccgct gacgctgctg tcggttgagc
     2941 cgtccgatgc cttcgtcgtc tgtgaggtcg ggacgaatgc accgggcgag atcgccgcgt
     3001 tggccaagct ggtcgagccc gaaatcgccg tcgtcacagg tgtttcggag gcccacctcg
     3061 aaggcttggg aagtctcggt caaatcgcag aggagaagct ttcgttgctg cactacctgc
     3121 ggccggaagg cttggccgtc gtcaatgccg actgcgaggt gcttcgcaag accttggagc
     3181 gggatcggcg actggcgggg ctcaagtgcg tgacgttcgg cgagtggaag ggcgccgaca
     3241 tccgcatgac cagccttcag gttagtatgg ccaagaaagc tgaccggagg gtgagtcaga
     3301 cgcttccgcc ggtccatctg gagttcacgg tgaatgaccg gttcaagtac gagcttcagg
     3361 ttcccggtcg gcacaatgtt ctgaacgctc ttgccgccat cggcgtggct cgacgtctgg
     3421 gcgtcgatca cgaggagatc gccgcccgct tggcgacgtt ttcgttgcct cccatgaggc
     3481 tcgagtatca gcgcgtcgga gacttgaccc tgatcaacga cgcctataac gcgaaccccg
     3541 cctcactggc agcggccgtg gatgttctgg tcagcctgcc caccgagggg cgaaaagtcc
     3601 tgattgtcgg tgacatgcgg gagttgggtc aggcatcgga tgaactgcac cgtcaggcgg
     3661 ccgagaaaat cgccggcagc ggcgtgaaca tggtcatttc cattggagag aaatcccggc
     3721 tggtcagtcg aaccatcgag gcggtctccg gcggagcagt ggagacgcac gcttactcgt
     3781 cagcagtttc ggccaagcgg cggctcgtgt cgtatcttaa caaaaatgac accatcctga
     3841 tgaagggctc gaggctgctg gcgctggaga agcttgttcc ggtgatcagg gaatgggcgg
     3901 ttttgcctga ggggaagacg cccgtcgggg cagggagcag gaagaagctg cgctcgtcgg
     3961 ctgcattgtg agaacaagag acggtctgcc ccggtcgtcg gctcccgaag gggccggtcc
     4021 ggccgggcct gcatgaaacg ggtctggacg tgctttacca tttgttcgac gcactatatg
     4081 gtcaccccgc cgggccttac gcctacaagg acccgctctt tcggggaaca tgtgcgttat
     4141 tgttgggctt ctttctggtc tggttcttgg ggccatggtt catccggcaa ctcagacgct
     4201 acagcattgg cgaccaggcg gagttcgacc acgcccagct taaccttgac aacgccgata
     4261 agcggaacac gccgaccatg ggcggcctgc tgatcatggc ggtgatcctg tgtaacgtgc
     4321 tgttgctggc cgatctgaac aactactaca tcaccatggg gttgttctgc atggtctggt
     4381 tgacggcttt ggggtctgtg gatgactggc tcaagctgac catcaagcgt cgcacgggga
     4441 cccgcgacgg gctgaagatg cacgagaagc tcctgtttca gatcgccttg gctgtgctgc
     4501 tgggctttta catttaccgg catggcgaat acaacgccac cacgatcagc ggcgatcaga
     4561 tccagacatt tcgcattctg aacgtccctt tctataagac cggctgggag ctgaaccagg
     4621 tcgcgttctt catcatcacc atcctggtgg tcacgggcac gtccaatgcg gtgaacctga
     4681 ccgatggcat ggatggcctg gcttccggat gcatggtgct ttgtagtttc gtgttcatga
     4741 ttctggccta cattggcggc gatcgcgaac tggccaacag cctccttttc ccccacatcc
     4801 cgcacacggc ggagttggcg gtgctgtgcg gtgcgatgat gggcgcatgc atgggttttc
     4861 tctggcacaa ctgttatccc gccaaggtct tcatgggaga caccggatca ttgccgctgg
     4921 gcgggatgat cggttatgtg gcaatctgca cccggcagga gctgatgctg ttcatcgtcg
     4981 gaggcgtgtt cgtgatcgag gccgtgtcgg tcattatgca ggtgggttat ttcaagatga
     5041 gcggcgggaa acgcatcttc cgggtcgccc cgattcatca ccacttccac ctcggcggtt
     5101 ggaccgagac gcagacggtc acgcgtttct ggctcctggc gatcgtcttt gccggaatcg
     5161 ccctggcaac agtgaaaata cggtaggggg cgaactctgg agtcgcgccc ggcgaccgag
     5221 ggtatcggcc gagggaccgg gggcagcctg acgtctccct ttgacccggc gcgtctcgcg
     5281 cataccatga atcatcatgt tgccctgtga gatgcttcgg atccggcatt cggaccacta
     5341 tgaggcgttc gcgagattgt ggctgcctcg atcgccgcgc ggggcagtct tgtacctgca
     5401 tggtatccag tcgcacggac tttggtttga ggcttccgcg gcgcacctgg ccgaggcggg
     5461 gtttgccgtg ctgctgcccg atcgtcgagg cagcggccgc aacgaggtcg aacgcgggca
     5521 cgcctcgtcg gcccggttgt tgctccgcga tgtcgctgac gggctggacg aactgcacgt
     5581 gcggaccggg ttgccgcgat ttcacctgtt gggcgtgagt tggggtggca aactggccgc
     5641 cgccttttgg aatcatgctc cggctcgggt cgcaaccctg acgctggtcg cccccggatt
     5701 gtttccccga gtggacgtgc ccctgacgca aaagattcgg atcggcttgg cgtctgtcgt
     5761 cgcgaggaga aaactggtcg acattcccct ggatgagccg gagctcttta ccgccaatcc
     5821 cgcccgtcag gcattcatca ggtctgacaa gctgaagctg tcccgggcga ccgtggcgtt
     5881 catgcttgca tcgcgtcgtc tggacgcccg ggtggcgcgg ctggccaggc actcgccggg
     5941 gtgcccgctg aaggtattcc tggccggcag ggaccgcatc atcgacaacg agaagacgaa
     6001 agcattcgtc cggtctctcc cgtggccaac caggggcgtc gtcgagtatc ccgaggctca
     6061 ccacaccatc gagttcgagc ctgatcctcg cccgtttctt gacgatctgg ttgcctggat
     6121 gtcctcgcaa gccgatgtga agagtggaga agttctcgcg gcgggcccct gatggcgccg
     6181 tcggaggaag agttctgtga tcacgtattc actgcaaagc ggttcgaacg gcaacgccat
     6241 ctatgtcgag gcgggagacg ttcgcttgtt gtttgacgcg ggcgtatcgg gcaagacggc
     6301 cgagggccga atgcgcgtcc gcgggcatga gatacgcgat gcgaccgcct tgatcatttc
     6361 gcacgaccat gccgatcaca tgcgttgcgc gggcatctac cagcgaaagt tcggcctgcc
     6421 gatctacatg agtgcgcgga cgcatcgggc ctatcgcgag aatctgggcc acctgaagga
     6481 tgtacggtac tttgaagtcg gacagccgct tcgtttcaac ggcgtcgttg tctataccgt
     6541 ccccaccccc cacgatgccg ccgacggtgc agtgtttgtg gttgagcatg ccgggcgccg
     6601 gcttggcatt ctcacggatc tcgggcactc tttcgcggcc cttcgcaggc tgctgccgac
     6661 gctggatgcc gcgtacctcg aaagcaacta cgatccgcat ctgctggcaa cggggccgta
     6721 cccgctctcc ctgcaggctc gaatccgagg cagcggagga cacctctcca accaggaggc
     6781 tgcggcactt gtccgcgagt cggccaagcg tctgcgatgg gtggttcttt cccacctgag
     6841 cgagcagaac aaccggccgg agctggccct ggaggctcat cgtcgcgagg tcgggccgcg
     6901 gtttccgctc tatgttgctt cccgctacga cctcgggccg ttgatgagtc tatgaaggtg
     6961 aagctacact tcatctggcc gcttggaaat cgcaaatgac tggccgtttg ggatgaggca
     7021 aacggaaggc tcttgagccg acgcatgatt ccgtggcata atgatatgtg agggcgggga
     7081 cgatcccttg gacatggggc acatcgagcg aggacgtcaa aatgccgcga agaaagcgaa
     7141 cacgaggtgg catgcggcgg gccaaggcac ccgttgccgc attcacactg attgaagtac
     7201 tggtggtggt ggcgatcatc gcgctgctga tcgccatcct gatgccctcg atccgggcag
     7261 ctcaggacgc cgcccgcgcc agtgtttgcg gcaacaatct gaaacagtgc ttaacggccc
     7321 tccagattaa ccttgcggag ataaccgcgc gcagggagac cgaacgcatc agcacgaact
     7381 acgggtgggc tgcgattgtc taccggtatt ccaatcgtca agccggcatt gtcacctgtc
     7441 ccagcgatcc cgagccgtgg cccatccccg cgttgtacgt gagaatactg ggggagcgga
     7501 gcgagctgct gtccggtgat gctctgtaca atcgcccgaa gttcatcgct gagacccgct
     7561 atgaacttga catacaggat gtggtcgcgg ggcgcggttt tggaaacgat gcatggaaca
     7621 ccaatgacat agaccttctg ctggagtatg aagctcccaa gaagggcgac aacgctccgg
     7681 tcaaggtgaa gaacaaggag tcggctcttg gctttgacgt tctggattac agaaaaagga
     7741 cagtctgggc caatgtctct ggtcccattt cgacaccggt gggaatgccc ttgttgtgga
     7801 tgagctacgg ggccaacgct gccgcgggtt tgaaaggagt gagagggaac ccgatactgt
     7861 tggtcgaact gcctaagccg ggcgtgtttc cggaaaagta cggtagtttc cctgcggacg
     7921 acatgaagaa gagccttcgt tttcgtcacg gcgagaaggc tccgccgtca gccggcctag
     7981 gtggctggaa ctacaaaacg gacgtcgtga acggcaaagc cggcggcgaa gggtatgtgc
     8041 cccgcattcg gatgaacgtc gggttctatg acgggcatgt tgagcgaatg caccatttgc
     8101 agatgatcaa gagcccgccg ggcaatcttt ggttgggcaa ccgcgctccg gggtacgtgc
     8161 ctgagttctg gtgatttcct ttatgtcctc ccgtcatagc ggtcaccaaa cggtcgagca
     8221 gacaagggag caggctggac tggcgcagag atagggcctc taagtttttt tgcgacaatg
     8281 ggatagcgac cgaacgtcgt tagccagggg gacaagacct catggcgggt tccggcaaag
     8341 gcctgttgga aggaccctgc ttttcatctc ttgacacgtt tcggaaaggc ggttatataa
     8401 gtgttggctg gtggaaaggc cacgcggtcc gacactggcc gagtccaaag gaattagagg
     8461 gacagcgaga gagagacctc acgctgtcct tttttcgttt ccggatttca gcctgagaag
     8521 ctggtggaaa ggagatgaat cgagagagct tgggttcgga caattgaata atggcctata
     8581 aatgtttccc gcgattcgcc cttttcagcc ccacacaagg aatgggaaaa gcatcttggt
     8641 tgggaaaagc gcggagctgg ggaaagcgcc ggagggcaag agcgcggaag ggaaaagcaa
     8701 gaggggagtc ccaaggagca agacgggaat ccctcctctc ggattcggag ctggggaaag
     8761 cctcctctcc cgaaagagct aggacacgcc cctctcccgt ggaggaatcg ggagaagcgt
     8821 tttctccctg ggggctaggg agccgccttc ggacgcggag gtggatacgc ctctcaacgc
     8881 ggatgaatgc ggggaacagc aatgccggct ctgttcaatc acagggcaga gccggcactt
     8941 tttgcgctca tcctggtttt gggctgttca cctgttctga aggtctctcc ccgtgacgat
     9001 tctttgacct tgcagcccgg tttagttagg ctaacagact tggtccggct tgggtcccat
     9061 ggcgattggg atcttggccg cataaagcta ttttgaaagg tatctcatga gatgcagcag
     9121 cgtactagcg attgcagcga ttgtctgcgt agtggcgatg gttacattcg gccagatcgc
     9181 tccaccaggt tcccagccgg catcgaaggc tgccactgcg cctgcggatg aggtggcggt
     9241 ggtggtgaac gaccggccca tcatggaaag cgagatcgac cgggtcatcg cccagggacg
     9301 aaagatcacc cctgaggagc tcgctcagaa acgacagcag atcaagccgc agcagatgaa
     9361 gttcttgttc gattacatca tcggcaacga actcatcgtt caggcggccg ggcgggaagg
     9421 tatcgttttg actgaccagg aatggaaaga ggagttccga aagaccttcg acaaatgggt
     9481 tcaagccaac ggctggtcgc aagagcaggc cgcccaggag atcaggacac ggtttgacat
     9541 ggatcttgcc gaactggagg aggaaatgcc caagtccccg ggtttccggg cgccgatcct
     9601 ccaggaaaag atcatgcaaa agaagttcgc cgatgaactc agggtcaccg acgaagacgt
     9661 caaagcctac tacgagcaaa acaaagcgaa ccgctacacg cccaaagaag aagaggttcg
     9721 agccagccat attctctttg cgaccaggga tgctaagact cgtcagccta tgtccgagga
     9781 ggccaagcag gaacagcgca agaaggccga acagatactg accgaggcaa agaagcccgg
     9841 tgctgatttc gccgccttgg ccagggagca ttcgagttgt cctaccggca agcgcagcgg
     9901 cggcgacctg gattactttc cccgaacgag gatggtgccg gcgttttccg aagccgcgtt
     9961 tgccatgaag gtcggcgaaa tcagcgatat cgtggagacg gagttcggat accacatcat
    10021 caaggtcacc gggcgcgtgg aggcggggga gactaagccg tttgatacag tcaaagacca
    10081 gatccgaacg caactggagc agcagaagtt gcgcacggtc gtggaccgtt acaaggaaga
    10141 gctcaagaac aaggccaaga tcgtttatcc gccgggcaag gagccggcca gcccgccccc
    10201 tgtacggcct gtgaccaccc ggccgacaac tcagtcgaca agggccgcca tgcgaccgag
    10261 agcgtcatcg accactcgcc ctggcgatcg cgcctcggta tccctgcctc ggcctcagac
    10321 agcggcggct gaataggcct gatcgcgaaa tgcccatgcc acgcccggat cgcgaggtcg
    10381 ccgttatccg aacgttcttc tttgcggggt ggttctactc tggcgcgacc aggttgtact
    10441 gatcaagcgc gatggcagtc ctgcccgtgt ggaacttcat cggctttgcg ccgggcggca
    10501 gatggaacaa gaacacataa gcgtatttgc ccttgagatc ctcgaaccgg atgtgctcca
    10561 gaccgggcat ccgcgcgaaa ccgcgggcgg tctcatcgag ataggtcaac tcgaagatgg
    10621 tctctccgcg aatcgtcgcg atggcgtatt tgccgacagg catgtactgt ttgccgttgc
    10681 cgtctgtcac gtagatgtct ctggttagct cggtcgcggt cccccgcgct ttgcccaggg
    10741 tgctccctgg ctgcaaccgc tctacgttta cgtgcaacaa gcgcatatcc gcgggcacat
    10801 caaagctctc caaaggtggt tttgagcccg ccacaggttc ccagtcggcg ttgagcttga
    10861 cggtgaagcc gcgggcgctt cgcagcttgc cgtctgaccg ctcaaactcc atgccttcgg
    10921 tgtagtccgt caaaggcgca gcaaagggca gccgttcaga gaaaaagggc tcctgggagg
    10981 ccaggttgac gccgtgaacc cggtctcggg ggttcggatc cggtcggggg ggcgagggac
    11041 gtctttcggt ggcggccgag gaggcgtccg tgtcgtcccg cgtgggtcgg ggctggcggg
    11101 acgttgagac atcatcacgc gattcgcgag gtctgtcccg gtcggagggt ctctccgtga
    11161 ggcttgtgtc tgagactgtg cctgcccgcg ttttggtctg gtcgtgcgac ggcaagatct
    11221 ctgcccaggc gttcatcttg tattggatgg caagcggccg gaaatcagga ttgtcgggaa
    11281 cctcaaagag aagccgcatc tcggggctgc cccccgcgcg gttgtattga aggtcctggc
    11341 aagggaatac ctgaatcaac cggtgggcgt tggccgggtc tgactcgttg atggctgcca
    11401 gcgaatagac tttcaccgga ccattgttcc ggtcgcgggc cagcagacgc acctgggacg
    11461 aggtgaaatt gaggtttgcg cggtcctggc atacgtccgt cttttcattg attgtcaccg
    11521 tcacacagag ccatcgctga ccctcctgcg gtttgcgatt gacgggctca tatttgaggg
    11581 tcacgacgct gttctcgtcc ctgactttgg ctcgcttgta gatcggctcg tcttttcgca
    11641 ggtattcata ttgtttgatg atcgccgcgt tgggcggcac ggcggccagc ggttcgcggc
    11701 ccaggccgtc tcgtatatgg tgaatatagt cgaggaagtc gggattgatt tcggcgaagc
    11761 tattacggcc gcgaagagcg aatcgcgaaa gatgcgacat cagagccacg gtgagcccgt
    11821 cggggttaag ccacaagttg tggcggattg ccttgacgtt ggaccagtcg atctcgttgc
    11881 gatagaccac gttcgtattc tgatcgccct tgcttgtggc cgacgatttc gtctcggtga
    11941 cgacctcctt gttggatgat gcgtccacca gactgtatcg gctgaacccg agcagcgtcg
    12001 ggtcaaacgg cagcatctga aaaccgatgg cgagcatgcc gacgatgatc atggcggtga
    12061 tgaagccgaa gatgccgccg cccgcgcgat ccaggtagat ggggaacgtc tggttgccct
    12121 tgatcaagag atcaacgacg gtgcgcatca cgagaagcga gacgatgaag atggccatca
    12181 gtgcgatcgc cctgccgtgg tcgggctggt aggtaatcaa ctgcgactga taaaggtctt
    12241 cgtagtaggc gaaggccagc gccgccgcca ggatcgtcag tacgcagttg atcaaggcgg
    12301 aaaacatccc ctgcagcccc tggtagaagg tcacggcgag gatcaggacg gtcgcaaaca
    12361 gcgagaacca cattgcgagt cacccccttt cctttccggc accgtcgctc ttggggttct
    12421 tcggtttcga cacggaagct gcgggtggcg gctgactgtt tccgcctgcc tcctcctcgc
    12481 gcatgacgcg aagcaactcg gtcatgctgg ttaccccgtt catgaccttc tgcagaccga
    12541 cctcctgcag gtagagcatg ccgttcttgc gggccatcgc ccgaatggcc gataccggct
    12601 gacctttggt gatcagatcg cgcaggccgt cgtcaatctc cagcagctcg aacacgcccg
    12661 ttcgaccgaa gtacccgctg ccttggcagt tcgtgcacag gatcgggttg cctttggcgt
    12721 cgacgagtcc accgggtggc gggcgataga agtggtcgat cttgtcggcc ggcaggttgg
    12781 ccttcttcag caggttcggg tccggcttat acggttgccg gcaggcgatg cagagcttgc
    12841 gaaccaatcg ctgtgaagtc agcgccacca gagccttggc aaccgtatcc agatcgcccg
    12901 ccaggctcat cagtttcttc agcgcgtcga acgtgtcgcg ggcttggatg cccatgtaga
    12961 tcttcttgcc gtcacaggcg gccttggccg ccaggtgggc cgtctcgcgg tccgggcaat
    13021 cgctgaccat caccacatca ggctctcgtc ggagaacggt ctgtagctgg cgggcatagc
    13081 ttgcctcgtg cttggtcgag tcgtaaatgt gttgggtgat gttctcaagg tccatcagcg
    13141 gttcccgctc cagcgtgagc aggttttgca tgaaggcgtc gtggttgcgc agggccgcgt
    13201 agagcgttga tgtcacgccg ctgccgtgcg ggccgctcac gatgaccagc ccggagggtt
    13261 ttcgaatgat ctcctccatg gctgccaatt gcttctcatc catgcccagg tcaccgatcc
    13321 gcaaccggtt ttcctctccc acgatgcgaa gcgagagttt ctcatactgg gtggtgccgc
    13381 tggtacgcac ttcgatctcg gtcggctggg cattcgtgcc ggtgatgacc gcactcatct
    13441 caccctgttg gggtcggcgc cgttcctcga cgtccagacc agcgacgacc ttgatcaggt
    13501 tcagggcctg tagggcggcg tcgcgtgtca atagatcggt gcgaggggtg gcgacgccgt
    13561 cgatgcgata tgccagtctg acgtcgctgg ggctgatcaa catctccacc tcggtggccc
    13621 gccgccagag ggcgtcgaag agcaggtttt gcgtcgcctc gaaagcgtcg gtctgcgtcg
    13681 ggtcggtggg gacgggcacc ttttcgccat tgtgacgggt catgcggacc cgctcgaacg
    13741 ccgtggcctt gccttccgtc ttcttgccca gtccgcccag ccagctcttg atgtgccgga
    13801 cggtaaaaac tcgggcattg gcgtcaacca gaccgtttcg aagcacaaca taggttgcgc
    13861 acgtccctac ggtggcgagg aaccataacc cgaagccggc ggcgaagagc ccgacgctgt
    13921 tccacggcat caggatccac acgaccagcc cgacggtacc gccgcccagg atgacgccat
    13981 tccaggtgtc gcgcctcatc cgacgcacgt actgggtatc cttgtcaacc cattggcaga
    14041 aggccatcca tggcagaagg cacaccagaa ccaaggcaat ccgccacggg ctcatatagc
    14101 cgcccggttc agacagtgcg gccagtgcac ttgcggcgag cgaggaggag gggaccgtaa
    14161 gcaccaggcc cgcactgagg gaaacagcgg tgaatcgacg tttgctgacg ctcatggcgg
    14221 ctccgaacat ataaacgaca tttcggtgct gagattgccc gccatcccgg ccgacggcgg
    14281 ggccgctgtc atcgcggcta ttctaacttg ccggttcccg ctcgtcacgc cattcggggc
    14341 ggattatccc aggatcgacc ccgcacccgc gctgatgccc ttgagacgca tcttaagctc
    14401 ctgcggattg ggcgaggcgg cataggccac cgccgtctcg atgtactcct gttccaccag
    14461 cctcttcagg cattcgttca tgtccagcat tccctcactc tgactggccc tgatgacctt
    14521 cccaagctcg tcgtcccgct tctcgtcaat cagcttcctg acggtcggcg tggcaaacat
    14581 gatttccact gccggaacac gggcaaggcc cggtttgata ctgggtaaca gtttctgaca
    14641 gatgatcgct tggagattga acaccagggt ctggcggacc aggtcgcggg aaccttccgg
    14701 aatcaggtcc aggatgcggt taatggtctg ggaactgctg gacgcgtgca cggtgccgaa
    14761 gaccaggtgc ccggtctcgg ccgcgtttag cgcggcggtg aaggtttcct cgtcgcgcat
    14821 ttcgccgatg agcaccacgt ccggatcctc gcgcatgagg tatttcaagg cgtcggcgaa
    14881 gctgtagacg tcgacataga cctcccgctg gttgatcagc gctttcttgt ccgtaaagag
    14941 gtactcgatc gggtcttcga tggtgacgat gtgacaggcc cggtgggcgt tgatgtagtc
    15001 caacgcggct gcgatggttg tcgacttgcc cgagccggta atgcccgcca ggagcacgag
    15061 cccttgatgg aactcggtca ctttgtagat cgacttgggc aggtgaagct ggtcgaaatt
    15121 caaaatgttc ttgttgaccc ggcgggccgc gacgctcaac tggcctcttt gtcgaaagat
    15181 attcacgcga aatcggtcgg aatcgcccaa ctgataggca aagtccgaag ctcctttctc
    15241 agccagttgc tttcgctggt gctcgttcat gatctcgaac cagaggttct cgacttcctg
    15301 cgggcttagc ggctcaccct taatcggttt caggtcaccc ttgatccgga tatgaacgcg
    15361 ggcattgctc ttgaagtgga tgtccgaggc ctggatgtcc accacgtact tgagatactt
    15421 gtggatcttc ggttcgcgct tggcggaggc tgctacggtc ctgttcttct cgggcgctgc
    15481 ggattttact ggtgccggcg ccgggggctc ggacggttcg atctcttcca gtgcgggggc
    15541 cgcctcgacc ggctcatcgc cgacatgcgt ccgttcgttg atccttcggt aaatgtcctc
    15601 ctcagccatg tacgcgcact ccgtgggttt ggtttcctgc cgctctagag ccgcacgggg
    15661 atgccccggt cggccatgta ctgcttggtt tctcttatcg tgtatgttcc aaagtggaag
    15721 atgctcgccg ccaggacggc atctgctttc ccgatcgaca ccgcgtcagc cagatgcttg
    15781 ggctcgccgg ccccgccgga ggccacgacc ggtatgctga ccgcctcagc caccgcccgc
    15841 gtgatctcaa ggtcgtaccc ctcctgggtc ccgtcggcgt ccatgcaggt caggacgatt
    15901 tcgccagccc ccagttcctc aacccgcttg gcccaagcca cggcctccag accagtgggg
    15961 gtacggccgc cgtggatgtg gacgtcccag aattcccggc cagctttcca tacccgcttg
    16021 gggtcgatgt tgaccaccgt gcagcatctg ccaaaacgcc gagccgtctc ggttatgatg
    16081 ttggggttca ataccgccgc cgtattgatt gagacctttt ccgccccggc ctggaccagt
    16141 ctggttacgt cttcgatggt ccggatcccg cctcccacgg tgaagggcat gaaacactcc
    16201 tccgccacgc gccggaccac ctccaacatg atgtcgcgac cctcgtggct ggcggtgatg
    16261 tccagaaaga ctagttcgtc tgctccagct tcctcgtagc cccgggcaat ttcgaccggg
    16321 tcgccggcgt cgcgcagatt gatgaaattc acgcctttga caacccgtcc ggcgtgcacg
    16381 tctagacacg ggataatccg tttggccagc atccggcaat cgttcccagg taagaatccg
    16441 ggcagttcag tgaggccacc tgcggggacg gccaaactcc tgcttcgggg ggtcattcgg
    16501 gccgtcaggg aacattctat ccgaggccga agttcccttt caatgtcttg cgtccagtga
    16561 agttgtgcca gtctcggaat atgttgcgct cctgcccggg actatcctcg cagtggggtc
    16621 ctgcccttcc cgattagccg acatgcgtac caccaagccc aggcgtggac ggtctctgag
    16681 gctggcacag cctggtcctc aacggggttg ctgaccaggg tgaacaccgg tccaccgttg
    16741 aggtggccca ttggctctgg cgggctcacg gtcggaatgc agtcataagc tcttttttga
    16801 aaaagggtta tgatggtgcg tccggcgtgc ttgcggaatc atggttcgat tcgcggatcg
    16861 acacacccgc ttgcggccgg ggatacacac tggggacagg gcccgaacgc ttggatcccg
    16921 ttgttggcat tccatttgga gccccgctga aacggtagcc ccattccgct ttcaatcgtc
    16981 gcaggcagga ttggccggaa ttccagggcc gctggcacaa tcttcgaact tgtacaggtc
    17041 ggcctggtca atgtcaccgt cgggttcgag cggttcgggg cgatcgaagc aggcgcactc
    17101 agccgggagg gtgaagctgc ccggcccggt gtagcaagcc tgcagggctg cgaagtcagc
    17161 ttggtcgacg tccccgtcct gatcggcgtc ggcaaacgga tcccagttca ggcactccag
    17221 gggaacgaac gatgaccgcc gccaagagtc gaattcgcac tcatcctgga agacggtttc
    17281 ccggtcgcag attcctgctt gtccgccgtt ctcgaagtcg ccgttgctga tcaggttcgg
    17341 tccgccattt tgcgtgacga ccacattgtc ccacagcgac tccatgcttc cacccgggtt
    17401 gcgcccggct ttgagaacaa cggtcacgac cgtaccggtg ggcgtgatgg tttgcggacg
    17461 cggacccgct ggtccggtgt cggcaggagt caggtgcgtg atctgctccc acgcgaacga
    17521 gccggcgccc agcacgcgct tgcggatcat gtatggattg ttggccgagg tgctttcctg
    17581 aaactggtcc gcatactgca tgctgaacgg cccgtcgaga agtatgagct cgaaccaact
    17641 gctctcgccg gccgatgcgc ctttccagta gtacgaatac tcc
//
LOCUS       DAOTPW010000113        17617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3221_length_17617_cov_5.026307, whole genome shotgun sequence.
ACCESSION   DAOTPW010000113 DAOTPW010000000
VERSION     DAOTPW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17617
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3221_length_17617_cov_5.026307"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="P5316_11635"
     CDS             <1..586
                     /locus_tag="P5316_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85649.1"
                     /translation="AKGIVTGDLGDRPQLLKQRAQSKVAFIYAAVAAGRYSEAAGELT
                     WLLTPVDSNVPTRKADAATGQLPDPLFLTTVKNIREKYGDKRAFDAHLAELGGYVAAA
                     QRQAAVAGGQGATRELVELLALRAFMLWSDGENRESRINARFTANQLPLPWRALSQAM
                     ATAEAALYGGRLEGSGTGGRATDVRLPWERSEGS"
     gene            820..2853
                     /locus_tag="P5316_11640"
     CDS             820..2853
                     /locus_tag="P5316_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRR85650.1"
                     /translation="MPMADSDQHNGSGVHFSSRVLLSILGHTVLFSLALLLAFGLRYD
                     FRGAAADESWFFKQYLPYLPCSLAVKLTVFGLFGIFRDSWRYVGLRDLFSIVRACHIS
                     IFVCVILYAVYTWLLVAFSQGREWLQERYLLEIPWLVFLQDWVLTIGLVAGARIGFRF
                     YNEELRPLESTSQARLLIVGAGDAGDSVLREIIRSSEDRYQVVGFLDDDPNKQGARIR
                     GVSVLGTTADIRQICESHDVDEVLIAMPSAPQRRVRELIEKCKGSNLLFRTVPAISDL
                     IEGRVEVSQIRPVSIEDLLGREPVTLDTHAIGEYIRGKVVMVTGAGGSIGSEMCRQIA
                     RFQPKMLLLVERMENALFEIDRDLQRLFPDLCRLPCVADIADAPRIRGLLRKHRPTAV
                     FHAAAHKHVPMMEWNPGEAIKNNVIGTRILADCASEAGVEKFVMISTDKAVNPTSVMG
                     CTKRIAEMYVQQLGSHTGSKTEFVTVRFGNVLGSSGSVVPIFKEQIARGGPVTVTHPE
                     MTRYFMTIPEAAQLVLQAGAMGKGGEIFLLDMGEPVKIVDLAFDLITLSGLRPNEDIE
                     IRFTGMRPGEKLFEELSIAGEDVSPTQHPKIGIMRKRPEDFNRIRQRIDVLAAMADTA
                     SAEELRAELAATVPEYVPYVSPSPHGVDRASQAARTGERPVSARVAPAGPGAA"
     gene            3075..4322
                     /locus_tag="P5316_11645"
     CDS             3075..4322
                     /locus_tag="P5316_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85651.1"
                     /translation="MSDRDSYREGMAAYHAGRYEDAVRKLTPLASDGEGATRVMSRFY
                     LGQAHYRLAVRFFDQRLFRDASHHFRLAAQANPSGGGFAGFLARCCRGDKRLAETAHR
                     YAEWVRHHPADAGSRVRLALAQWKIGQPGQAMATLQEGLAACPGNADLHYQMGVLFAA
                     NEQFHEARQCFEKALEIDPSHAGAHERLAQCHGVAGDARAAAEELQKAHALDPENARV
                     AMQISVLAGTITDRNLRLSTIPQPGDSSPRYDQADIERLGEAVVAEPDFVDAFLQLPK
                     TEVDQEVFNVLRATLEKALEKHPEYADLHYHCGRVYHRLGGEAKAIRHIEKAVEINPR
                     YVDALILLARLYGQTDRWGHGIERLRQAIDAGADYPDVHYLIGRLYQKANQPEQARQA
                     YRRALSLKSDFPAAREALEALGT"
     gene            4335..6026
                     /locus_tag="P5316_11650"
     CDS             4335..6026
                     /locus_tag="P5316_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85652.1"
                     /translation="MSYLLEMLGRGWLGRLSDAFQDRLTRIPARDCETLQALIEQNPE
                     EHYLHMELGFALLARHRISQARNAFGAALKLNPSSILALVSRACCQEEMGLFDDAAND
                     LQAACNLLPDDVAIQFGLGYCRERAGRTNEAIEHYQASLRLCPSLRNAHERLAAIYLR
                     QNRIEEAVEQYEEMLVDEPGDVDLHITVANLYLRLGRAEDAIREFERALLIEPDNWGA
                     RNDMAETLEQAGLYREAIEQLHMMCEQRPECAETHLRLGDLYAKIGHDEAALKSYTRA
                     LELHPGYLEATVKVGTQHLRSGRYVDAAAFFSRAIEINDRLLTAYVGLGVAQHSAGRQ
                     AEAWSSFDLAASVEPNTTLLYSEMARIHLKCAAQQEAEASLHLSPPDEVGPPEDRISD
                     LIDRQIERHREMVRERPAHADLHYRLGLLLRQRGRLEEAIAQFQQAVRINPVYSKALI
                     KLGLALYEAGRSDEAIHALQEALALHPEYIELHYRLGVMFADRQQFRLAVEHFEQAVA
                     ANPQNVEFRESLALALESMGMIDRAKAMWHRICEMSPKSEHAQRARHAIFRHTAG"
     gene            complement(6063..7457)
                     /locus_tag="P5316_11655"
     CDS             complement(6063..7457)
                     /locus_tag="P5316_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85653.1"
                     /translation="MSGTSSSKATSRREFLKTSGRIAAVSALAGTAVPAVHAAGSDTI
                     QLALIGCGGRGGGAVNNAFAVKGASMKLVAMADVFENRLKGSYEGLSKGRADKVDVPP
                     DRRFLGFDAYKKAMDCLKPGDVVIFATPPAFRWVHFAYAIEKGLNVFMEKPVTVDGPT
                     SRRMFALGEAAEKKNLKVGVGLMSRHSRHLEQLAKRVHDGEIGEIVLQRGYRMAGLIG
                     SFASTPKPEGISHLMYQIQRFHSFLWSGGGCFSDFNIHIIDHLGWIKNAWPVRAQAVG
                     GRHYKVNDKGVPYVDQNFDSYSVEYTYADGTKFFFDGRCMPGCSDFYASYLHGTKGSG
                     IASAGGDCGAPSSLYKGLSIKDSDKIWESKNLPGDEGPYQNEWNDLVDAIRNDKPYNE
                     VKRGVEASLVTSMGRMAAHTGREITYEQILNSDHEFAPGLDKLTEDSPPPLQPDADGR
                     YPVPMPGKKTDREY"
     gene            7760..8293
                     /locus_tag="P5316_11660"
     CDS             7760..8293
                     /locus_tag="P5316_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRR85654.1"
                     /translation="MTNRVTASAAAFEVYPLYRRPAHLGRAGRRGSVAGFGLVFLVLL
                     SGGCGGSKLIPLHSSADFQRHVLDSRKPVLVQFYKSGCMWCSLQEPALDRLAGDYSGR
                     AVFAKYCLKDWLGGVTNWEIRSKYDIGWYPTVILFVHGREKKRWVTHFDEKSYRKALD
                     EVLGTPKPQVFVAPAKR"
     gene            8365..9972
                     /locus_tag="P5316_11665"
     CDS             8365..9972
                     /locus_tag="P5316_11665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR85655.1"
                     /translation="MESINLGLCNKCRGRVPCEFFARDGQMWLRKTCPDCGSTESMVS
                     SDARAWQAKRELWEYVPMQRVACRMNCDRCEIDHKPNIVFVDVTNRCNMNCPICIATI
                     KDMGFDFNPPLAYFDKLFAEVARLEPKPVLLFFGGEPTVRDDLLEIIAAAKKHGLRPH
                     VVTNGIRLADEQYCRRLCEARVSFRFAFDGRSPEIYERLRRNRSAYDKKMRALQNLRK
                     YSLRKHTIMATIGRGFNDQHLGDFFQFCHENRDLISDVGLIPLTENWRPGTFDAGVHT
                     TMEDVEAMVRDAIPGGQVEFVPAGLSWALKTPRSFFRKNLRSEVLLLAGVHPNCESIT
                     LLVSDGKSYRGMNHYLRKPLREVAVECAALCRKLQPKLDRLDPARRFPRLCGQVLILL
                     TLLPWMLVRIRLGRLLGSHLSALGGRVFGRPGRLLSGGKHIRRRPRLVLRVGVLPFEE
                     EHSIDAARLEKCKAVFAYEDAEDGSVKYIPACLWYPYRNPILEKLSKKYGVVGKETEW
                     TIPEQPYNSSGGNPTVKPLTVKAPICR"
     gene            10316..10936
                     /locus_tag="P5316_11670"
     CDS             10316..10936
                     /locus_tag="P5316_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR85656.1"
                     /translation="MFGYIKTAFVLSAILFATLGCGCPEVEQAPAEVPPPVLKEPPPP
                     PTPEPLPPAVTKSIEELNEKYPDLFKFDKDKGLLYFSADATFDSGSATVKPEAKAALA
                     KLAAILNEDEVKDRRVTLVGHTDTDRVVKPATIAMLKELGKSVDNMGLSRARAEAVAA
                     VLEVNGIDASRITTIGKGETEPVADNRKPEGKARNRRVEIYVTPAK"
     gene            complement(11284..12240)
                     /locus_tag="P5316_11675"
     CDS             complement(11284..12240)
                     /locus_tag="P5316_11675"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRR85657.1"
                     /translation="MIGRDAFAGRLTEAQQELVVQNIGLVGLHLRNRVPTPRRPTRMR
                     ERADLFQVGCMALIRAAIRYDPASHGPFPAYALPRIRGAIFTAVHEYFATIRVPRDVI
                     VRRRKELALNGRTSIQRLPIVHQWRGQANIVTPHRAVTETQEWGDPTIRALARSRFER
                     AVQAALEELKSRRWRRRDTSEVMARIAAERILVSPSPQQTRTRQLSSLLGVSSGRISE
                     YEQLLRETIQQHLSRDALLPLLLQFAREDPAGMDGCITCERRERLHTAEMKAFESQLA
                     AMERSRRAELLYGLVEQSGKKPDDVIRKLYRRQVWNKSLVPM"
     gene            complement(12562..13422)
                     /gene="surE"
                     /locus_tag="P5316_11680"
     CDS             complement(12562..13422)
                     /gene="surE"
                     /locus_tag="P5316_11680"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRR85658.1"
                     /translation="MRFLLTNDDGYDAPGLRALHEAVGSLPGVEIGIIAPAVAQSGTS
                     HSTSESFICRQVCLEGMGQITVAEGTPADCVRAALHLPGHPRPDWVIAGINRGSNLGV
                     DIFYSGTVAAAREAAILGVPAIAISQLVKPSMPDDWLSSARLAAAIMAAITLPEQPPS
                     PWVDQKCFDMVRRALLDAALSAAALSPSHPVAPVRPMTGFPSQGCPAAPCWNVNLPRS
                     SDGRPPLGACVAPISSDPPAFGLTPMSQGNGITRLTYVGRYHERQATPGTDVALAFAG
                     YITLSPLALV"
     gene            complement(13435..14496)
                     /locus_tag="P5316_11685"
     CDS             complement(13435..14496)
                     /locus_tag="P5316_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRR85659.1"
                     /translation="MAVVSRPIRIIGTKTVFPVPCRAGLSADGMTGEEYSRTVYTAPN
                     VGRRDAAGDDRQPDLRERDKARLQMNEKVYLNGDIIDLAKAVVTVSNPSLLHGVGLFE
                     TLRAYDGRAFLLQRHIDRMTDSAKHFDMPVLQLVEQIPDAVAAVLAANRLKNARIRFT
                     VMPPGAQDGHERSTLLVAAQETAGYPPVLYERGMTVHLNTRWRQSDLDPLAGHKTTSY
                     FSRLLALRDAQAAGCGEGLWFTPENRLAQGCISNVFVVKEGVLRTPPLDTPVVPGITR
                     QVVIELATEAGVKTEQVPLTVNDLLDADEVFLTNSVMEIMPVTRIERKAVSNEKPGPI
                     TGQLQQAYNAMTRQTKSTS"
     gene            14847..16187
                     /locus_tag="P5316_11690"
     CDS             14847..16187
                     /locus_tag="P5316_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRR85660.1"
                     /translation="MNLHTRRAAWMSWVITASLVASALGQTTQPAAAGRMIQGKIKGT
                     NVNVRSGADTNYYVVTKLNRGDTVTIVHESFNWVEILPPKGCFSVIDKNYVEKADDGS
                     GTLNGDAWVYAGSEIDERRYAKQVQLKKGEKVQILGETADGKCYKIAPPLGATVWVHG
                     DFVDRPGATGGDQPATGERIPPGTLSLAQAGVTTSRPAKEPALTPVAKGSEPAFGENI
                     VKTTGLEPIKPELLTTKNDEHRAAIAKIEAGIDAESAKPAKQRNFEQFIKDLEPLASQ
                     SDDEVAKLYAETRIKQIRSLAEASRAIEEMRRLKNEAEEKADAERVRLEELRRKRIEE
                     FPTDWVDVRGEIRLSGLFDGTGGRSKRWRVVEPGVTPAKTLAYIEVPEGSSINPSDYL
                     DKYVEIKAEARKLSSAAMPVPVYTVRQIKLAERPARGKPPAGVMTRPAGPVTTK"
     gene            16248..16919
                     /locus_tag="P5316_11695"
     CDS             16248..16919
                     /locus_tag="P5316_11695"
                     /inference="COORDINATES: protein motif:HMM:NF016503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85661.1"
                     /translation="MFKSRNCAGWVVVFLAGALAAGCGFLPRGYVFDPSDLEEIVDQI
                     TATSGEDAVQQVHDALLDRGYPVATEPNWVINSGRGVPGQVAAFYLYANEYLVVAGVN
                     PGAQWSMAPGLGFEVHDYVLFGDLTAYAAGELNPPTLGPGDSLSLGPFESRQFRTDTG
                     VWMLEYGRGLLPTDLYPEAFAGEFALAQNAILYASGSFVTSFIRNWVYEQIGLPGGIF
                     SLYGL"
     gene            complement(17347..>17617)
                     /locus_tag="P5316_11700"
     CDS             complement(17347..>17617)
                     /locus_tag="P5316_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85662.1"
                     /translation="TRLGWVSVRRVIGLFTGLPLHEPWITIRDIRPRTLVVVAIIFVL
                     WTPPLRSTDGKAGTGGFPILSDPHSKRWHPDASAQDDRSSNASDG"
BASE COUNT         3369 a         5214 c         5580 g         3454 t
ORIGIN      
        1 tgccaagggt attgtcacgg gtgatcttgg tgatcgaccc caactgctca agcagcgagc
       61 acaaagcaag gtcgcattca tctacgccgc ggtcgccgca ggccgctatt cagaggccgc
      121 cggcgaattg acctggctcc tcacgcccgt cgacagcaac gtcccgaccc gaaaggctga
      181 tgctgcaacg ggtcagttgc ctgatccgct atttctgacg acggtgaaga atatccgcga
      241 gaagtacggg gacaagcggg ccttcgatgc tcacctcgcc gagctgggcg gatacgtagc
      301 agccgcgcag cggcaggcgg ccgttgcggg aggccagggg gcgactcggg aactcgtgga
      361 actgcttgct ctcagggcat tcatgctgtg gagtgatggc gagaaccgag agtcgaggat
      421 caatgctcga ttcacggcaa accagttgcc gctgccctgg cgagcactct cgcaggcgat
      481 ggctacggcc gaggccgccc tgtacggcgg ccggctggaa gggtcaggca caggcgggcg
      541 agcgacggat gtccgccttc cctgggaacg ctcggaggga agttgaagat cccgcaagcg
      601 gcgcaaacgg gcggccaaca gcataatgga cttgaacaaa gaaatgcccg ataaccaggg
      661 aaggtttgcg gcggtgtgca gacacctcgc acgaagggag aattcgtttg aggaagctgc
      721 tgccgggctt atgttgccgc atgttttgtt gtgaagtggt tgtcgaacgg ggcccaggat
      781 ggccgggggg aagttggcgg attgggggag gaagccagaa tgccgatggc ggattcggat
      841 caacacaatg gctccggtgt gcatttttcg agccgtgtgc tgctttccat attggggcac
      901 acggtgttgt tcagccttgc tctgctgttg gccttcggtc tgcgatacga tttccgcggg
      961 gccgcagccg atgaatcgtg gttcttcaag cagtacttgc cttatctacc ctgctcactc
     1021 gcggtcaaac tgaccgtttt cggtcttttc ggcatattca gagattcctg gcgctacgtg
     1081 ggtcttcgcg acctgttctc cattgttcga gcctgtcata tcagcatttt tgtgtgcgtc
     1141 attctctatg cggtctacac ttggttgctg gttgcgtttt cccaggggag ggaatggctg
     1201 caggagcgat acctgctcga aattccctgg ctggtgttcc tgcaggactg ggtgctgacg
     1261 atcggcctgg tcgccggggc gagaatcggc tttcgtttct ataacgagga actcaggccc
     1321 ctggagagca cgtcccaggc aagattgctg attgtcggtg ccggtgatgc cggtgattcc
     1381 gttcttcgcg agatcatccg gtcatccgag gatcgctatc aggtcgtggg tttccttgat
     1441 gatgatccga ataaacaggg cgcaaggatt cgcggtgtaa gtgtactggg cacgaccgcc
     1501 gacatacgcc agatttgcga atcgcatgac gttgatgaag tgttgatcgc aatgccgtcg
     1561 gccccccagc gccgggtgcg cgaactgatc gagaaatgca aaggcagcaa tcttctcttt
     1621 cgcacggtac cggcgataag cgatctgatc gaaggccggg tggaggtcag tcagattcgc
     1681 cctgtgagca tcgaggacct cctcggccgt gaacctgtga ccctcgatac ccatgcaatc
     1741 ggtgaataca tccgcggcaa ggtggtcatg gtcaccggag ccggcggctc gatcggctcg
     1801 gagatgtgta ggcagatcgc ccgcttccag cccaagatgc tcctgctggt ggagcggatg
     1861 gagaacgcct tgttcgagat cgaccgtgac ctgcaaaggc tttttcctga tctctgccgc
     1921 ttgccctgcg ttgctgacat cgccgacgct ccccgaatca gggggttgct tcggaagcac
     1981 cgtcccacgg cggtgttcca tgccgcggct cacaagcacg tgcccatgat ggagtggaac
     2041 ccgggcgagg cgatcaagaa caatgtgatc ggcactcgca tcctggccga ctgcgcctcg
     2101 gaggccggag tcgaaaagtt tgtgatgatc tcaaccgaca aggccgtcaa cccgaccagt
     2161 gtgatgggat gtacgaagcg cattgccgaa atgtacgtcc agcaactcgg cagccacacg
     2221 ggctccaaaa cggaattcgt caccgtccgg ttcggcaacg tccttggcag tagcggcagc
     2281 gtggtgccga tcttcaaaga acaaatcgcc cgaggcggac cggtcacggt gacgcacccg
     2341 gagatgaccc gctatttcat gaccatccct gaggcggcgc agctcgtttt acaggccggg
     2401 gccatgggca agggcggcga gatctttctg ctcgacatgg gcgaaccggt gaagatcgtc
     2461 gacctggcct tcgacctgat caccttgagt gggcttcgcc ccaacgagga tatcgagatt
     2521 cgcttcaccg gcatgcgacc gggcgagaag ctttttgaag agctctcgat cgcaggcgag
     2581 gatgtcagcc ccacccagca ccccaagatc ggcatcatgc gcaagcgccc ggaggacttc
     2641 aaccgcatcc gtcagcggat cgacgtgttg gcggcgatgg cggatacagc ctcggccgag
     2701 gaactccgcg ccgagctggc cgcaacggtg cccgagtatg tgccgtatgt ttcgccatcc
     2761 ccccacggtg ttgaccgtgc ttcccaggcc gctcggacgg gggaacggcc cgtatccgcc
     2821 cgcgtggctc cggccgggcc cggcgccgca taagaccttc ctgaccccac cccggatgca
     2881 ttcagcgccc tgcaggcttt tatcgggctg tttcagcagc gatccggtcg gtccgaatgg
     2941 cgcaattgtc ttattctgcg ctgcttaacg ccgcggttgc cgtaaccgat acaaccacag
     3001 gccgggacga accgcgctga cggccgttat cgcgaacgac aacgcggcca ggccacgcgc
     3061 accacgagga tgctatgagc gatcgcgatt cctaccgcga ggggatggct gcgtatcatg
     3121 ccggccgcta tgaggacgcc gttcgcaagc tgaccccttt ggcctcagac ggcgaagggg
     3181 cgacacgcgt gatgagtcgt ttctatctcg gccaggcgca ctaccgcctc gccgttcggt
     3241 tcttcgacca gcgattgttt cgggatgcca gtcaccactt tcgtctggcc gcgcaggcta
     3301 acccttcggg aggagggttt gccggattcc tggcccgctg ttgcagaggc gataaacgct
     3361 tggctgagac agcccatcgc tacgccgagt gggtccggca tcatcccgcc gacgcgggca
     3421 gccgagtcag gctggccttg gctcaatgga agatcggaca gcccggtcag gcgatggcga
     3481 cgctccaaga aggtcttgcg gcttgcccgg gcaacgccga tctccattac cagatgggcg
     3541 tcctgttcgc cgcaaacgag cagtttcatg aggccagaca atgctttgaa aaagcattag
     3601 agatcgatcc ttcccacgcg ggagcccatg agcgactggc gcagtgccat ggcgttgccg
     3661 gggatgcgcg agcggcggcg gaggaactgc agaaggccca tgcgcttgat ccggaaaacg
     3721 cccgagttgc gatgcagatc agcgtccttg ccggaaccat caccgaccgg aatctgcgtc
     3781 tcagcactat tccgcagcct ggtgattcct cgccacgata cgatcaggcc gatatcgagc
     3841 ggcttgggga ggccgtggtc gccgaacccg actttgttga cgcttttctt caattgccca
     3901 agaccgaggt ggatcaggag gtcttcaacg tccttcgggc gacgctggaa aaggcgttgg
     3961 agaagcaccc cgagtacgcc gacctgcact accactgtgg gcgggtctat caccgtctcg
     4021 gcggcgaggc aaaggccata cgccacatcg agaaggcggt ggagatcaac ccgcgatatg
     4081 tggacgcgct tatcctcctc gcacggctct atgggcagac cgaccgctgg ggccacggca
     4141 tcgagcggct gcgccaagcg attgatgccg gcgcggacta tccggacgtg cattacctca
     4201 tcggacggct ttatcagaag gccaaccagc cggagcaggc ccgccaggcc taccggcgtg
     4261 ccctgagcct caagagcgat tttcccgcgg cacgcgaagc gcttgaagct ctcgggacgt
     4321 gaagagggga atgaatgtca tatttgctcg aaatgctggg tcgcgggtgg ctgggacgct
     4381 tgtcggacgc ctttcaagat cgtctgacgc gcattcccgc ccgcgactgc gaaacgcttc
     4441 aggctctcat cgagcagaac cccgaagagc attacttgca tatggagctc ggctttgcgc
     4501 tgctggccag gcacaggatc tcccaggcac gtaatgcgtt cggggcggcg ctaaagctga
     4561 accccagcag catcctggcc ctggtcagcc gggcctgctg ccaggaagag atgggtctat
     4621 ttgacgacgc cgcgaacgat ctgcaggcgg cctgtaacct cttgcccgac gatgttgcca
     4681 ttcaattcgg gttggggtat tgccgggaac gggccggtcg aaccaacgag gccatcgaac
     4741 actatcaggc gtctcttcgc ctctgcccct cgttgcgaaa cgcccacgaa cgcctggcgg
     4801 ccatctacct gcgccagaat cgcattgaag aggccgtcga gcaatacgag gagatgctcg
     4861 ttgacgaacc cggggatgtg gacttgcaca tcacggtcgc caatctctat ctccggctgg
     4921 ggcgcgcgga ggatgccatt cgcgaattcg aacgagccct gctcatcgag cccgacaact
     4981 ggggcgcccg gaacgatatg gccgagacgc tcgagcaggc cggcctgtat cgcgaagcga
     5041 tcgaacagct ccacatgatg tgcgagcaac gccctgagtg cgccgaaacg cacctgcgac
     5101 tgggcgatct ttatgcgaaa atcggtcatg acgaggcggc cctgaaatcg tacacccggg
     5161 ccctcgagct gcaccccggc tatctggaag cgacggtcaa ggttgggacc caacacctgc
     5221 gatctggccg ttatgtcgac gcggctgcat tcttcagccg ggcaatcgag atcaacgacc
     5281 ggctcctgac agcctacgtg ggtctgggcg tggcccaaca ctctgccggg cggcaggccg
     5341 aggcgtggtc gagctttgac ctggctgcca gcgtggagcc gaacactaca ctgctttact
     5401 ccgaaatggc ccgcatccac ctcaagtgcg ccgctcagca ggaggccgag gcgtccttgc
     5461 acctgtcacc gccggacgag gtcgggccgc ccgaggaccg catctccgac ctgattgacc
     5521 gccagatcga acggcaccgc gagatggtac gggagcggcc ggctcatgcc gatctgcact
     5581 accggctcgg tttgctgctc cgacagcgcg ggcggcttga agaggctatt gcccaattcc
     5641 agcaggccgt ccgcatcaac ccggtgtaca gcaaggctct gataaaactt ggcctcgccc
     5701 tctatgaagc cgggcggtcc gatgaggcca tccatgccct gcaagaggcc ctcgccctgc
     5761 atcccgaata cattgagctg cactatcgac tgggagtgat gttcgccgac cggcagcagt
     5821 ttcggctggc ggtcgagcat ttcgagcagg cagtcgcggc caacccgcaa aacgtcgagt
     5881 tccgtgaaag cttggcgttg gccctggaga gcatggggat gatcgaccgg gccaaggcca
     5941 tgtggcaccg gatctgcgaa atgtccccca agagcgagca cgcccagcgc gcccgccacg
     6001 ccatctttcg acataccgcc ggttgaccgc agcgttcccg cggccgattg ccgggcctcg
     6061 tctcaatact cgcggtccgt cttcttgccc ggcatcggca ccggatacct gccgtcggcg
     6121 tccggctgca gggggggcgg cgaatcctcg gtcagcttgt ccagcccggg cgcaaattcg
     6181 tggtcggagt tgaggatttg ctcgtacgta atctctcgac ccgtatgagc ggccatgcgg
     6241 cccatggagg tcaccaggct ggcctcgacc cctcgcttga cctcgttgta cggcttgtcg
     6301 ttgcggatgg cgtccacgag gtcgttccat tcattctggt agggaccttc atcgcccggc
     6361 aggttcttgg actcccagat tttgtccgag tccttgatgc tcagtccctt gtagagactg
     6421 gaaggggctc cgcagtcgcc gcccgcggag gcaatccccg agccttttgt gccgtgcagg
     6481 taactcgcgt agaagtcgct gcaaccgggc atgcagcggc cgtcgaagaa gaatttcgtc
     6541 ccgtcggcgt aagtgtactc cacgctgtag ctgtcgaagt tctggtccac gtagggcacg
     6601 cccttgtcat tcaccttgta gtggcgaccg ccgaccgctt gggccctgac cggccacgcg
     6661 ttcttgatcc atcccaggtg atcgatgatg tggatgttga agtcgctgaa gcacccacca
     6721 ccggaccaca ggaagctgtg aaaccgctgg atctggtaca tcaggtggct gatgccttcg
     6781 ggcttgggcg tcgacgcaaa gctgccgatc agcccggcca tgcggtagcc ccgctgaagc
     6841 acaatctctc caatctcgcc gtcgtgcacg cgcttggcca gttgctccag atgccggctg
     6901 tgccgagaca tcagccccac gcccaccttg aggttcttct tctccgccgc ctcacccagc
     6961 gcgaacatcc gccttgacgt cggaccatca acggtcaccg gcttctccat gaagacgttc
     7021 aggcccttct cgatcgcata ggcgaagtga acccaacgga acgccggcgg tgtggcgaag
     7081 atcaccacgt cgcccggttt caggcaatcc atcgccttct tgtaggcatc gaaccccagg
     7141 aatcggcggt ccgggggaac gtccaccttg tcggcgcggc ccttactcaa gccctcgtaa
     7201 gaacccttga gccggttctc gaaaacgtcg gccatggcca ccagcttcat agacgcaccc
     7261 ttgaccgcga aggcgttgtt gactgctccc ccgccccggc cgccgcagcc gatcaaagcc
     7321 aactggatgg tgtccgagcc ggccgcatgc acggcgggca cggccgtgcc tgccagagcc
     7381 gagacggcgg cgatccggcc ggaggtcttc aggaattcgc gtcgcgacgt cgccttggat
     7441 gatgaggttc cgctcatgat ggttctccat ataaagaatg gatcgaggtt tacggctggg
     7501 attataccag cccgatggcc ggacggtcca acagtcgcgg caggtggctc tttgacaccc
     7561 aacacgcccc cgccaccgga ccgttgccat gcgaggtttc ggctcttgac tgtcagtgtt
     7621 cagtgcggct aagaatgtgt ctccaaacgg tggggcacgg tcggagccgc tgaaggtggc
     7681 gcaaacctgc cagtcttggt taagcctttt ccggcgagtc catgcgaatc gaagtgaaca
     7741 tcatgaaagg gacaagctga tgacaaacag ggttacggct tccgcggcgg ctttcgaggt
     7801 gtacccgcta taccgccggc cggcccacct tggccgcgcg gggcgtcgtg gatcggttgc
     7861 cggattcggc cttgtctttc ttgtccttct ctccggaggc tgcggcggca gtaagctcat
     7921 ccccctccac agttcggccg atttccagcg gcatgttctc gattccagga agcctgttct
     7981 cgtgcagttc tacaagagcg gatgcatgtg gtgctcgttg caggaacccg ccctggaccg
     8041 cttggccggc gattacagcg gccgtgcggt tttcgctaag tattgcctga aggactggct
     8101 tgggggcgtt accaactggg aaatcagaag caagtatgac atcggctggt atcctacggt
     8161 gattctgttc gttcacgggc gggagaaaaa gcggtgggtg acgcatttcg acgagaagag
     8221 ctaccggaag gccttggatg aagtcctggg taccccgaag ccgcaggtgt ttgtcgcgcc
     8281 ggccaagagg tgagttgttc tgacgggacg accgtcgcgg cgaggctgtc ggatcgccca
     8341 gaggcaaagg gagacaacgc ctgtatggag agcatcaatt tgggtctgtg caacaagtgc
     8401 cgcgggcgcg tgccttgcga gttctttgcc agggacgggc agatgtggct tcggaaaacg
     8461 tgccctgact gtggttcgac cgagtcgatg gtaagttccg atgcgcgcgc atggcaggcc
     8521 aagcgcgagc tctgggagta cgtgcccatg caacgcgttg cctgccgcat gaactgcgat
     8581 cgatgcgaga tcgatcacaa gcccaacatc gtcttcgtcg atgtcaccaa tcgctgcaac
     8641 atgaactgtc ccatatgtat cgccacgatc aaggatatgg gtttcgattt caacccgccg
     8701 ctcgcgtact ttgacaagct ttttgctgag gtggcgcgcc tggagcccaa gccggttctg
     8761 ctgttttttg gcggcgagcc tacggtccgc gatgacttgc tggagatcat tgccgccgcg
     8821 aagaaacacg gtctgaggcc acacgtggtg accaatggca tcaggctggc ggacgagcaa
     8881 tactgtcgta ggctctgtga ggctcgggtt tcgttccgtt ttgccttcga cggccgcagt
     8941 ccggagattt acgagcggct tcgccgcaat cgatccgcct atgacaagaa gatgagggcc
     9001 ctccagaacc tccgtaagta cagcctgcga aagcacacga tcatggccac catcggacgg
     9061 ggtttcaacg atcagcatct tggcgacttc ttccagttct gccacgagaa tcgggatctg
     9121 atttcagacg tcgggctcat tccgctgacc gagaactgga ggccgggtac gttcgatgcg
     9181 ggggtgcata cgacgatgga agacgttgag gcaatggtcc gagacgctat ccctggcggg
     9241 caggttgagt ttgtacccgc cgggctctca tgggccttga agacgccgcg gtcgtttttc
     9301 cggaagaacc tgcgatccga ggttctgttg ctggccggcg tacaccccaa ttgcgaatcc
     9361 atcactctgc tggtttccga cggcaagtcc tatcgtggca tgaaccacta tttgaggaaa
     9421 ccccttcgcg aggttgcggt ggaatgcgcc gctctttgcc gcaagcttca gcccaagctt
     9481 gaccggcttg atccggcacg acggtttccg cgactctgcg ggcaggtgct gatcctgttg
     9541 accttgttgc cgtggatgct ggtgaggatc agattgggtc gcttgcttgg cagccacttg
     9601 tcggctttgg gtggccgggt atttggccgt ccgggtcgcc tgttgagcgg tggtaagcac
     9661 atccgacgaa ggccgcggtt ggtcctgcgc gtgggtgtgc ttccgtttga ggaagaacac
     9721 tcgatcgacg ccgcccgatt ggagaaatgc aaagcggtct ttgcctatga ggacgccgaa
     9781 gatggcagcg ttaagtacat tccggcttgc ctatggtatc cgtaccgcaa cccgatcctg
     9841 gaaaaactct caaagaaata tggcgtcgtt ggcaaggaga cagaatggac gatcccggaa
     9901 cagccctaca atagcagtgg cggcaatccg acagtcaaac ccttaacggt taaggcgcct
     9961 atctgtcgat agaaaggtat tgaagcccac tcgatcggcc ggtgtcgaca gaccactatg
    10021 gtcggaggcc agggacgcgg cgcgagcctt ataccaggcc tgtcgcaggt cggcggggct
    10081 tagagtcttg cggccagaca tctgtgcggc ttgcgcgcac gtttctggat cgcggtgtat
    10141 gtggattgcg gcccgcaaaa aggcggtctg ccttacgtca actggttgtg cgtttctggg
    10201 attaactctg gttgcgaacc ggggacggct gggctggtgg cggcgggtga tcccgaccgg
    10261 gctgggccaa tccgtccgaa atatgccggt caacccaatt caaggagaga tggcgatgtt
    10321 tgggtacatc aaaactgcgt tcgtgctgtc tgcgatattg tttgctacct taggttgcgg
    10381 gtgtccggag gttgagcaag ctcccgccga ggttccgccc cccgttctga aggaaccgcc
    10441 acctccgcct actccggagc cgcttccgcc ggccgtcacg aagtcaattg aagaactgaa
    10501 cgagaagtat cccgacctgt tcaagttcga caaagacaag ggactgttgt atttcagcgc
    10561 cgatgccacg tttgactctg gttcggccac cgtgaagccc gaagcgaagg cggcgctcgc
    10621 gaaactcgcg gctattctca atgaggatga ggtgaaggac cgcagggtca cactcgtcgg
    10681 ccataccgat accgaccgag tggttaagcc ggcaaccatc gcaatgctta aggagcttgg
    10741 caagagtgtg gacaacatgg gtctgagccg ggcccgggcc gaggcggtgg ccgctgtgct
    10801 cgaggtgaat ggcatcgacg cctcgcggat caccaccatc ggcaaggggg aaacggaacc
    10861 ggttgcggac aaccgcaagc cggagggcaa ggcccgcaac cgtcgcgtag agatctacgt
    10921 gactcctgca aaatagtggg atccgtgtcg gcccccatcg ccgtattcga ggcgcgagaa
    10981 gagacatcgt caaagaggcc gccaaccgaa ggtccggcgt ttgtcgctgg tcgctgccgg
    11041 gcagggtggc tctttgtctg tgacggtccc catcggcggt gggggcccaa caggcatgca
    11101 gccaacaagg tcgaccaggg catgggaccg gagagtggcc gtgcatccca agccaatatg
    11161 acaaaccatc cggaacgtat tcaagacatc tccttttgcg gcgtgtatga gataatgggc
    11221 gccccgttga tcgcgtctcc gcgtgcattg caacatttcc gcccgggcag cacgacaaga
    11281 aggctacatc ggcaccagac tcttgttcca tacttgtctg cggtatagct tgcggatcac
    11341 gtcgtcaggc ttcttgccgg actgctcgac gaggccgtag agtagctcag cgcgccggct
    11401 tcgctccatg gccgccagtt gactctcgaa tgctttcatt tcggcggtgt gcaggcgctc
    11461 gcgacgttcg cacgtgatgc agccgtccat ccccgcaggg tcttcgcgag caaactgcag
    11521 gagaagcggc agcagcgcgt ctcggctgag gtgttgctga atggtttcgc gaagaagctg
    11581 ctcgtactcg ctgatgcggc ccgaggagac gcccaggagg ctcgatagct gccgtgtacg
    11641 cgtttgttga gggctcggtg acacaaggat tcgctcggcg gcgatgcgag ccatgacctc
    11701 ggaagtgtcc cgtctacgcc acctgcggga tttcagctct tccaaggcgg cctgcactgc
    11761 tcgctcgaat cggctgcgag ccagcgcccg gatcgtcgga tcaccccatt cctgcgtttc
    11821 cgtgaccgcc cggtggggag tgacaatgtt cgcctggccg cgccactggt gcacgatcgg
    11881 caaccgttgg atcgacgtcc ggccgttgag agcaagctcc tttcgccgac gcacgatgac
    11941 gtcacgcggc acgcggatgg tcgcgaagta ttcatgcacg gccgtgaaaa tcgccccgcg
    12001 gatgcgcggc agggcgtagg cgggaaacgg accgtgtgaa gccggatcgt accttatggc
    12061 tgcacggatc agtgccatgc aacccacttg gaacagatcc gcgcgctctc gcattcgggt
    12121 cggtcggcgg ggtgtcggga cgcggttgcg gagatgcagc cccaccaagc cgatgttctg
    12181 aacgaccagt tcttgttgcg cctccgtcag ccgaccggca aaagcatccc gcccgatcat
    12241 tcgctggtcc gacccggctg ttggcgaggg tcgccttgat tcggaggatc gaattgccgg
    12301 accgtttttc gcctccggga ttggtcccgt tgtctcctcc cgttccgttt tgcgctgccc
    12361 tgatccctct tcttgagtac cccctcgaca ccatcgacag ttcgccctcg ccgcaacagg
    12421 gaatcccgcc ccaaccttca tcgccatccc ctccgccccc tcaatggaca tttcgcatat
    12481 acctaacata aaccaaacat acgacaaagt caagcggccc aatccaattt gtcccttgtg
    12541 gatctcagtc atcagggcaa gtcacaccag ggccagtgga ctcaaggtga tgtatccggc
    12601 aaaagccaat gccacatcgg tacccggggt ggcttggcgt tcgtggtatc ggccgacata
    12661 cgtcaagcgg gtgatgccgt tgccctggga cataggggtg aggccgaagg cgggcggatc
    12721 ggagctgatc ggagcgacgc aggcccccag cggcgggcgg ccatctgatg atctggggag
    12781 attcacgttc cagcagggtg cggccgggca accctgggat gggaaaccag tcatcggccg
    12841 gacgggagcg acagggtggc ttggggacaa ggctgcggcc gatagggcgg cgtcgagcaa
    12901 cgccctgcgc accatgtcga agcacttctg gtctacccaa ggcgacggag gttgctcagg
    12961 caaagtgatc gcggccatga tcgcggcggc aaggcgcgcc gagctgagcc aatcgtcggg
    13021 catcgatggt ttcaccagtt gcgagatcgc gatggccggc actccgagga tggcagcctc
    13081 gcgggcggcg gctaccgtac ctgagtagaa gatgtcaacg ccgaggttgc tcccgcggtt
    13141 gattccggcg atgacccaat ccggccttgg atgaccgggc agatgaagcg ccgcgcggac
    13201 gcaatcggcc ggagtaccct cagccaccgt gatctgcccc atgccttcca ggcagacctg
    13261 gcgacagatg aaactctcgc ttgttgaatg gctcgtgccg ctctgagcaa cggcgggggc
    13321 gatgatgccg atctctacgc cgggcaggct gccaacggct tcatgaaggg ctcgcaggcc
    13381 tggggcgtcg taaccgtcgt cgttggtcag gaggaaccgc aagataccat cgcctcagct
    13441 tgtcgatttg gtctgtcgcg tcatcgcgtt gtaggcttgc tgtaattgcc cggtgatcgg
    13501 tcccggcttc tcgttgctga ccgcttttct ctcgatccgg gtcaccggca tgatctccat
    13561 gaccgagttg gtgagaaaga cctcgtcggc atcgagcagg tcgttgacgg tcaggggaac
    13621 ttgttcggtc ttaacgccgg cctctgtcgc aagttcgatg accacttgac gggtgattcc
    13681 gggcacaacg ggagtatcca gcggcggggt gcgaagtacg ccttccttaa cgacaaaaac
    13741 gttgctgatg catccctggg ccagccggtt ttcgggggtg aaccacaatc cttcgccgca
    13801 gcccgcggcc tgtgcatccc gaagagccag caggcgggag aaatagctcg tggtcttgtg
    13861 cccggccagc gggtcgagat cggattgtct ccaacgggta tttaggtgga cggtcatccc
    13921 ccgttcatac aggacgggcg ggtagccggc cgtctcttga gcggcaacca ggagggtaga
    13981 tcgctcgtgg ccgtcttgcg cacccggcgg catgacggtg aatcgaatgc gggcgttttt
    14041 gagcctgttg gcggcgagca ccgcagccac cgcgtctgga atctgctcca ccagttgaag
    14101 cacaggcatg tcgaagtgct tggcagagtc ggtcatccgg tcaatgtgtc gttggaggag
    14161 aaaagctcga ccgtcatagg cgcgcagggt ttcaaagagg cccactccgt ggagcagcga
    14221 gggattcgac accgtcacga cagccttggc taggtcgatg atgtcgccgt taagataaac
    14281 cttttcattc atctgcagcc ttgccttatc gcgctcgcgc aggtcgggct ggcgatcatc
    14341 gcctgcggca tctcgtctgc ccacattcgg cgcagtatac accgttcgcg aatactcttc
    14401 acccgtcatg ccatcggccg acaagccggc ccggcacggt acagggaaga cggtttttgt
    14461 gccgatgatc ctgatggggc gacttacgac cgccacgcga ggaatccttg gggcttccct
    14521 ggagcgatcc ggccccctcc gaaccagacc gcatgggcag gttgtgtccg gctggcaaac
    14581 caaggccgcg ttgccacgcc ggttggaaac agcccgcaag tgacgctgcg aggactggtt
    14641 atgcggccgg tgccgcaacg gttcagggta gccttggttg acatccgata accagagatg
    14701 tactgtgcac accagtggcg aggcggtggc gcgcggccgt gctcgccggt tcgcatcggc
    14761 gacaaaccgc gtgtccggac cgctgagcgc cgggattgcg cggacacgga acgattaacc
    14821 agtacaaagg actgaggagc ggtgcaatga acctgcacac acgacgagcg gcatggatgt
    14881 cctgggtcat cacggcaagt ctggtggcct cggcgttggg tcagaccacg cagccggcgg
    14941 cggccggtcg catgattcaa ggtaagatca agggaaccaa cgtcaacgtg cggagcggag
    15001 cggatacgaa ctactacgtc gtgacgaagc tcaaccgcgg cgacacggtg accatcgtcc
    15061 acgaatcgtt caactgggtg gagatcctgc ctcccaaagg ctgcttcagc gtgatcgaca
    15121 agaactacgt cgaaaaagcc gacgacggca gcggcacgct gaatggcgac gcgtgggtct
    15181 atgccggcag cgagatcgac gagcgccgat acgccaaaca ggtgcagctc aagaagggcg
    15241 agaaggtcca gattctcggc gagacggccg acgggaagtg ctataagatt gccccccctc
    15301 tcggggccac tgtctgggtt catggtgatt tcgtggaccg gcccggggcc acgggcggcg
    15361 accagcccgc gacgggtgag cggatcccgc ccggcacact gagtcttgct caggcaggag
    15421 tcacgacaag cagacccgcc aaggaaccgg cgctgacgcc ggtggccaag ggcagcgagc
    15481 ctgccttcgg cgagaacatt gtcaaaacga ccggtctgga acctatcaaa cccgagctgc
    15541 tcaccacaaa aaatgatgag catcgcgcgg cgattgccaa gatcgaggcc ggaatcgacg
    15601 ctgagtctgc caagcctgcc aagcagcgga atttcgagca gtttatcaag gatttggagc
    15661 cgctggcgtc ccagtcggac gatgaggtcg cgaaactgta tgccgagacg cgcatcaagc
    15721 agattcgcag tctggccgag gcctcaaggg ccattgagga gatgcgcagg ctgaaaaacg
    15781 aggccgaaga gaaggccgat gccgaacggg tcaggctcga ggaattgcgg agaaagagga
    15841 tcgaggagtt tccgacggac tgggtcgacg tgcgcgggga gattcgtctc agcgggctct
    15901 ttgacggtac tggcggccga tccaagcgct ggcgggtggt cgaaccgggc gtcacgcccg
    15961 ccaagacgct ggcatatatc gaagttcccg agggcagttc gatcaacccg tcggactatc
    16021 tggacaagta tgttgagatc aaagccgaag cccgcaagct gagttcggcg gcgatgcccg
    16081 tgccggtcta cactgttcgg caaatcaaat tggctgagcg gcctgctcgc ggcaaaccgc
    16141 cggccggggt gatgactcgt cccgccgggc cggtgacaac aaagtgagcc cgttcccagc
    16201 ggacggcatc attggcccgg gacggtggag cccggatggg ggatgacgtg ttcaagagtc
    16261 ggaactgtgc tggctgggtg gttgtcttct tggcgggtgc gctggctgcc ggttgtggct
    16321 tcctgcctcg cgggtatgtc ttcgatcctt ccgatctcga ggagatcgtc gaccagatca
    16381 ccgcgacctc gggcgaggat gcggtgcagc aggttcatga cgccttactg gaccgaggat
    16441 accccgtcgc aaccgagccg aactgggtga ttaacagcgg aaggggcgtg ccggggcagg
    16501 ttgcggcatt ctacctgtac gcgaatgagt atctcgtcgt ggctggtgtc aaccccgggg
    16561 ctcagtggtc gatggcaccc ggcctgggct tcgaggtcca cgattatgtg ttgtttggag
    16621 atctcacggc atacgcggcg ggcgaactca atcccccgac tctggggccc ggcgatagcc
    16681 tctcgctggg tccttttgag tcccgacaat ttcggacgga taccggcgta tggatgctcg
    16741 aatacgggcg ggggcttctg cccaccgacc tgtaccctga agcgtttgct ggcgagttcg
    16801 cgctggctca gaatgccatt ctttacgcca gcgggtcttt tgtgacctcg ttcatccgca
    16861 actgggtgta cgaacagatc ggcctgccgg gcggcatctt ctcgttgtac ggcctgtaag
    16921 gcgttagtca agcgacggct tccatttgtt tcctgttggg tggcccaccg cttcagcggg
    16981 ttcgccccgg cgtagcctgt ggcgaagccg ggtggggtag cgaactgtgg caggagttgc
    17041 cgaccccacg gctgaagcca cgggtcaacc gcttgggtgg ctgttactgc ggacaaaggc
    17101 cgctttgggt tatcgcccaa tataacctac tttcatgcgt ttcccgacat gattgggctg
    17161 ccctgccctt agggcggtga ggggtgcaag aactctgcga tggtgagaac tcgcttgcgt
    17221 ttttgcttgg ctaacgtagc atcgacgagg ccgaaggcct cgcttaagcg gggcgcccat
    17281 ctctcttgtc tcttgacaga cgcaagtccc tgaagcgccc gtggcacaaa tgacgctgtt
    17341 cgtgtcctac ccgtctgaag cattgctcga ccgatcatcc tgtgcggagg cgtcaggatg
    17401 ccatcgtttc gaatggggat cgctgagaat gggaaagcca cctgtcccgg ccttgccatc
    17461 tgtggaccgc aatggtgggg tccacaggac aaatatgatc gccacgacca ccaatgtgcg
    17521 agggcggatg tctcggatgg tgatccaggg ctcgtgcaag ggcagaccag tgaacagtcc
    17581 gatgacccgc cgtaccgaga cccaacccag ccgagtc
//
LOCUS       DAOTPW010000114        17574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3228_length_17574_cov_4.768023, whole genome shotgun sequence.
ACCESSION   DAOTPW010000114 DAOTPW010000000
VERSION     DAOTPW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17574
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_17574_cov_4.768023"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(772..1167)
                     /locus_tag="P5316_11705"
     CDS             complement(772..1167)
                     /locus_tag="P5316_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85663.1"
                     /translation="MGRILRYAWAGPASLLGILFVPLALVSGGGVCIIDGVVEVHGGI
                     VARLLRLGVVWGRPAMALTLGHVVLGCDAVCLSKTRAHERVHVGQYERWGPLFIPLYL
                     GASLVVLVAGRDPYRGNPFEREAFMQQGP"
     gene            complement(2114..3703)
                     /locus_tag="P5316_11710"
     CDS             complement(2114..3703)
                     /locus_tag="P5316_11710"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HRR85664.1"
                     /translation="MNAISRLLDGEPLSVTLQEQAAGELGGTCGFAVYPASVESTGTI
                     VVALGKEGRSRRLILACRRGKLGAFGSLSPEHRRSAGDVQVTVVSAGPDAASALREAV
                     PWTSPRLCGPATSVGLGDRLGLATPGHLLAVQDQGCVPFPAQQSIREMTRTQRTPQEV
                     MDDAVWGVFQAGWRGGFGSDADHLKTTADIDATAAAGFTMFTIDPGEHVCSEADTMSA
                     AAASMAANALPWADLETSEEDLRRRYLNRRFPLVGRGDVRFTEETLLRAAVKYGRAIA
                     HTAAMYRHLVARLGIGRFELEVSVDETDAPTSEAEHYLVAAELKRLGVRWVSLAPRFI
                     GAFEKGVDYRGDLKAFEQSYAAHAAIARTLGPYKLSLHSGSDKFSVYPIAARLSEGLI
                     HLKTAGTSYLEALRVIARVNSDLFRRIVSFALERFDADKASYHISARIERVPRPDDLK
                     DAELECLLDSDDGRQLLHVTYGSVLTAANPDGSHRFRQEVRETLIDHEKEHYAVLAGH
                     IRRHVVPFGWGRGAQGAGRGR"
     gene            complement(3725..4102)
                     /gene="rsfS"
                     /locus_tag="P5316_11715"
     CDS             complement(3725..4102)
                     /gene="rsfS"
                     /locus_tag="P5316_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRR85665.1"
                     /translation="MSKAKTRTAKPKNKALAFAKEAARIASDNHTEDIVILDLRGLSS
                     VADFFVIGTGTSDRQMRATCDDIEEYARTIGEKPYSVSGYDTATWLLADYVDVVIHLF
                     DAEKRKYYDLELLWGDAPRVNWE"
     gene            complement(4231..4836)
                     /locus_tag="P5316_11720"
     CDS             complement(4231..4836)
                     /locus_tag="P5316_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR85666.1"
                     /translation="MAVRHHIKVLVVDDDPQVCKMISTSLKDHGYHVQSYSQPRQALQ
                     AVRKAPFDMAIIDIKMPDLNGLEIVEKIKAEDPRIAPIVMTAYPDIQTAAETMRLGSR
                     DYLCKPFKEEDLLKAVERIAQEMGLVYTNEQELNRLIGQRIRQERLKQSLTLRQLSER
                     AELTTSQLSQVELGKNAASIWALARISSSLGKQLCELLAGL"
     gene            5546..6475
                     /locus_tag="P5316_11725"
     CDS             5546..6475
                     /locus_tag="P5316_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HRR85667.1"
                     /translation="MARKLSSIAPDWWDYTTLPEELLEEVARLTVDDVSRLSRDGFRV
                     VMYDTLEEFYLAEALEYIEAWRQATESQPVGICGPIGPTEQLPLVARLVNALELNLKH
                     AHFWGMDEWYKDGREVPETHPLSFAKADRELCFNRIRPDLRMPESNIHFPKADPTAYI
                     KSWEKVRCAVMQGGQGEIKHWAFNDPPKREAPYVDEPPPPEVYRKLTTRVVDLHPATL
                     MQNARTSGGGNVAGVPTKAISVGPVETWKAGRVSIWQAGTHDNPFGQRLTAFMISKEI
                     VDTSVPMSLLAQHPNVQFNYYRKGLGTCEAEMH"
     gene            6882..8207
                     /locus_tag="P5316_11730"
     CDS             6882..8207
                     /locus_tag="P5316_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR85668.1"
                     /translation="MSERHGHLLEAILLAAIGLILAYFLGVRLGWLPVFWGATFLLIA
                     VMLLLANLFWWAWVDRQLQGRGRRTGSTTRRRLLRSGWAAFMTLMLSPLLLVFVGLRG
                     FWDAYPFVLIALIMVWHLLMVLLVTVWAPLAGFWAMFRRVRARVAGSHSFEKPVDPSR
                     RQLLLGAVGVAPVIVAGGSLGLAVWQGGRFKVRRVSLRMPRLPDRLRGLTITHISDLH
                     VGRLFRPEHLPRLIDAVNQLKSDLIAVTGDLVDHSVDFMPLTCDALAQMESRCGRFIV
                     VGNHDLIDSPEKAVQELHRREPFLLLDRMLPLLIDGETIQIAGLFWSRYDEPRGEDPG
                     HTGRVTRTLAGADSDRFTIALAHHPHAFDPLADRGVDLTLAGHTHGGQLMLTPPGSHT
                     PIGGGSLLFRYIWGEYRRGDCVLEVSAGVGNWFPVRINAPAEITHIRLI"
     gene            complement(8250..9635)
                     /locus_tag="P5316_11735"
     CDS             complement(8250..9635)
                     /locus_tag="P5316_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85669.1"
                     /translation="MSIHFDRAWVLIEQSRPELAEQEARRGLAESPDDGRGHALLALS
                     LAQQEKLKEATAAVETAIHSAPDEPFPHYIRAAILHDRNRPEEALEAIHEAIRLDPEN
                     AYYHALLGQIRLTQRLWREALTAAEEGLRLDPEHVQAANVRAAALVKLGRRLEAAQAL
                     DAALARDPDDANTHANRGWAFLHEGKHRQALESFREALRRTAFLRTATQAFCDPETCA
                     QAAQISGRKVLRLEPGNEWARQGIVESLKAKNIVYGLMLRYFLWMGSLSGKARWGLIL
                     GGYFGARALSSVARSNPPLAPFIAPFLILYLVFVFLTWTAGPLFNLLLRLNRFGRLAL
                     TREQIVASNWVGGSLLLAVACLGLGFGLRSNSLLVASFIFGLLSMPLAGTFDCSRGWP
                     RTAMAFYTAVMALTGLGAVALYAWSRTRDSGASAEAIKGLVNSLAVGFAISFLASPWI
                     GNLLMMNRVRE"
     gene            complement(9632..10975)
                     /locus_tag="P5316_11740"
     CDS             complement(9632..10975)
                     /locus_tag="P5316_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR85670.1"
                     /translation="MAAGDDAIEALRDALRDAPDNVRLRQHLAEMLLRYGHTQQAVEE
                     YRETLNRCPDDPGLKLGLANAFYRDGKYSPALVIVEELLKSPSTAGEVYVLHARLLLR
                     AGDIDRAVRQYRRGIEQDPEAADDSLEETLGLNGEPDSEVIDGRVRVPGEQSSSGPDI
                     ELERPRINFDDVGGMEAVKEEIRLKIIHPLSHRELYEAYGKQIGGGILLYGPPGCGKT
                     HLARATAGEIKAGFLTIGINDVLDMWLGNSERNLHEVFQHARQHKPCVLFFDEVDALG
                     ASRADLRQSAGRHLVNQFLAELDGAQYSNDGVLILAATNAPWHLDSAFRRPGRFDRII
                     FVPPPDSVARSAILRILVRGKPAEEIDFDKLAAKTNGFSGADLKALVDQAIERKLQEA
                     MRAGVPKPMTTKDLLAAVAAVRPSTKEWFSTARNHAIYANQGGIYDDVLKYLKLL"
     gene            complement(11072..11821)
                     /gene="gpmA"
                     /locus_tag="P5316_11745"
     CDS             complement(11072..11821)
                     /gene="gpmA"
                     /locus_tag="P5316_11745"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRR85671.1"
                     /translation="MYKVVLLRHGESDWNKQNRFTGWTDVDLSEVGIKEAAEAGELLM
                     KEGYTFDVAYTSVLKRAIRTLWIALDKMDLMWIPVYRSWRLNERHYGALQGLNKAETA
                     AKFGEDQVKIWRRSYDIPPPPLEKTDPRYPGNDPRYADLTEGALPLTECLKDTVARFL
                     PYWHDVIAPTVKLGKRVLIAAHGNSLRALVKYLDNVSDKDIVELNIPTGMPLVYELDK
                     GLKTIKSYYLGDPEKVKAAMAAVAAQGKAKK"
     gene            complement(11936..12160)
                     /locus_tag="P5316_11750"
     CDS             complement(11936..12160)
                     /locus_tag="P5316_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85672.1"
                     /translation="MDCFVRLPIDEKVWNDLGRILRTLRSRGLAVPFADALIATVAMG
                     GGLLLWTYDAHFVVIASALPDLRLFDGPRI"
     gene            complement(12169..14370)
                     /locus_tag="P5316_11755"
     CDS             complement(12169..14370)
                     /locus_tag="P5316_11755"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRR85673.1"
                     /translation="MQRADRLIEEAIGRKELPGGVLLVGQGDRTVYFKAYGLRTSDPQ
                     PIPMTKDTIFDMASVTKTVACATSIMILVERGKLGLADSVAKHIPAFGANGKEKVTVE
                     QLLLHRGGFVPDNPMKDYADGPVKAWERIWNLAPVYPPGTDFRYSDVGYIVLGELVRV
                     IDGRPIDRFAREEVFLPLGMKDTMYCPPESLWSRCAPTEKRDGKWMQGQVHDPRAWAL
                     GGVAGHAGLFSTAEDLARWCRMILHKGVLDDKRILSQMTVEAMTSQRCLPGGTGCRGY
                     GFDMSTSYSSARGDLFQAGTTFGHTGYTGTSYWIDPTHDCYVILLTNRVHPDDKGTVT
                     DLRRQVATAVAAAITGKAFGPPAEVLCGIDILKRDGFKLLAGRKVALITNHTGRDREG
                     NRTVDLLFKAPEVKLVKLFSPEHGLYGTLDEKVGHTVDQATGLKVYSLYGETRRPTPE
                     MLEGVDALVYDIQDIGARFYTYSATLGYALEAAAKHKLKMVVLDRPNPITGLIVDGPI
                     ADKNRYGFTAYGPLPVVHGMTFGELARMCNVEYDINCDLTVVELQGWRRSMWWDETGL
                     MWINPSPNMRNLTEATLYPAICLLEATNVSVGRGTDQPFEIFGAPWIDGRKLAASLNE
                     AGLPGLRFIPIEFTPTSSKFAKEKCQGVYVLVTDRQNIRMVESGLTIAWHLKKLFGPV
                     FEVDKVINLLANAETLNNLKTAETPTTLPAGWRGSLSRFREVRQKYLIYQN"
     gene            complement(14576..15763)
                     /locus_tag="P5316_11760"
     CDS             complement(14576..15763)
                     /locus_tag="P5316_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRR85674.1"
                     /translation="MRQLSREEQQRACRLLADLVRIPSVVSTPEQANRDRAEERMAEY
                     LTDHLSRMGMQVERQEVFPGRPNLIAYWPGQGTGKRLVLSAHMDTVPTEGMTVDPFAA
                     EIRGGRMYGRGTCDTKGSLASLLTALALAHEAGRLPSDRLCFVATVSEETGCDGATAL
                     VRSGFTADAAIVGEPTNCEVVTAHKSPLWLEIETHGRSCHASLPTQGTNAIELMARVV
                     DFVGDPWKRHIGRRSHPLLGTSTCVVTTINGGSKINVIPSSCRAQIDGRFIPGEPADR
                     IVADFTRMLSDYLGGREKFSILSRRTHPPLDCPADKPLPRKLLTVCREANGQSAPRGV
                     NYFADTGPFSEAGIASLLFGPGDIAQAHTADESVELAQVYQATEIILALLTSHAGRSI
                     IDE"
     gene            complement(15787..16269)
                     /locus_tag="P5316_11765"
     CDS             complement(15787..16269)
                     /locus_tag="P5316_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRR85675.1"
                     /translation="MRLGILSDPHGNAAMVHRALVLLDARGAEAFILCGDIGGLNVLE
                     ELVGRRCWFVWGNTDCPDPTWRAQVESMGLPWPNGPLELRLEGKRIGVFHGNEKEFVK
                     AYERADRDYLLFGHTHRRHDSRSGGMRIINPGALHRVRVPTVALLDLRTDDLEFIEVV
                     "
     gene            16541..17302
                     /locus_tag="P5316_11770"
     CDS             16541..17302
                     /locus_tag="P5316_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRR85676.1"
                     /translation="MFAVITDIHSNIEALEVVLADIDRRGIKTIVCLGDIVGYGANPK
                     ECIDLIIERAQYCICGNHDAAVFYEPFNFNIGAERACYWTRQVLEDEPNKAIRDRRWE
                     FLGKLPPRLVYEGMLFVHGSPRRPVNEYLFGDDVYSNPAKLMSNFERFNEVACFVGHT
                     HVPGVFVDDPYFESPGELAEPGVYEIAEDDKVIINVGSLGQPRDRDPRAAYGIVDDGR
                     VEFVRLEYDVEKTVHKILSTPDLDDFLGHRLLEGR"
BASE COUNT         3312 a         5473 c         5222 g         3567 t
ORIGIN      
        1 cgtccaccct tccgaaaagg ccggcgtcga tccatttgtc taggtctttc cacctcgggc
       61 tggtgatcac gatttgccgg ccgatcaaga tggcctcgac ggcgagaata tgttgctgat
      121 gtactcgcgc acagcgcgcc tcagccgcgt cttgatagca gtgagaaccc agtcgacggg
      181 tacgcgggcc atcccggtat ccggacgaat gggaatctca agcctgccag gtcacgacag
      241 cttggcttcc atccgaacac ctgccgcccg acacaaagga cactatatct ctacatcagg
      301 tactacccgc atgtcaagcc gttctgggct cgtcggccac cttgggacgc gtgacagtct
      361 gggcgggcat gccgaccggt ccaggttggg caatcaaggc ccttcagcgg gtcttcccgc
      421 taaaggcggg ctgataggcc ggccctcata cggccggtcg gtggtcgcca ggggttcttc
      481 ttcctttctg tggtctcagc ccgtttcgat gggcttacct gacaaggaca ggcccgttgg
      541 aaccggcttg ttggggtaac acggtgaagg gggattgacc gaccgtgaat cagtcgtaaa
      601 acgaccggcc tgtttgccct tcgggtaaga ccgctgaagt ggtctggctt gcgttcacga
      661 caaccgggtc agccgccaga agtgccgccg cttctgtcac gggcccgcca ccttcgcggt
      721 caccctcggg cgcgacgaag tcaggttcca gcccgacccc tgccttgctt gtcaggggcc
      781 ttgctgcata aaagcctcac gctcgaaagg gttgcctcgg tacggatcac ggccggcgac
      841 caagaccacc aggctggctc ctagatacaa agggatgaac agcgggcccc accgctcgta
      901 ttggccgaca tgcacccgtt catgcgcccg cgtcttggac aggcagaccg cgtcacagcc
      961 cagcaccacg tgtcccagcg tgagggccat ggctggtcgg ccccaaacca cgccgagtcg
     1021 aagcagacgc gcaacgatgc ccccatgaac ttcgaccacg ccatcaatga tgcagacgcc
     1081 ccctccgctc accagagcaa gcgggacgaa cagaatccca agcagcgagg cgggtccggc
     1141 ccaggcatat cgcaagatcc gtcccatggt cacatccccc tggcagacgc cctcatcggc
     1201 gacttgcctg acgggctgta tcaatgcgcg gccctgctgt cggcaagcct aaacgtccct
     1261 gtccgatccc gcaagagagc gcgagttggc cttgcttggc cgggaatcct ttccgtgtcg
     1321 cctctcgccg cagaatcctt tccgcattca ccgtcaatgc gatgcgaatc ctcggccgga
     1381 agaccctggc tcggtagggc agcgccgagg tcgcttcagc gaccgaggcc tgccatatcc
     1441 tgtaattact gaacctttgc aaattggccg cacagcgttt gaaaaacatc tgtgccgctc
     1501 gatggaggca tggaagcgcc ggttggcatc cttcggcgca aaggatcggc tccgtcgtcg
     1561 gcccgcatct cgatagagtc tgtgtggtgg actactggac caagcgctga agctgtcgtc
     1621 acagtgaggg acctggagac cggcgccgaa ggaaaaaaga ggaaaggaac tgggttgaga
     1681 gcactgcgta tgccccagca agggtgtcac gggcgcattg gagaggatgt catatggcca
     1741 gtattcggag ccgccccgat ccggatgtgt ggaagggagg aatcgagatc tgcaaggcag
     1801 actaccggct ctcccggggg ttgccaaggc acgagtaact tggccgtggc agtcaaatga
     1861 agagagcatc cggtatcgat ccccggcaca attgcccagg gtcacgtgta gagtaacggc
     1921 acgcttcaga aatcatctgc tgaccgcaat ggggtcgccg cggagttgga aacgcagcct
     1981 atccctgcgc gcgaactata ttcacagaca gcccaggcgg cacaagatct gcatggccga
     2041 ttgcggtgcc ccaatcgccg gattcggact ctcttgaggc gactgccgga gtaaccctgg
     2101 caatagtgct ctttcacctc ccccgccccg cgccttgcgc cccccgtccc cagccgaatg
     2161 gcaccacgtg ccttcgtatg tgccccgcta ggaccgcata gtgctccttt tcgtgatcga
     2221 tcagcgtctc gcgaacctcc tggcgaaaac ggtgactgcc gtccgggttg gcggcggtca
     2281 acaccgaccc atacgtgaca tgcagcagtt gccgcccgtc gtcggagtcg aggaggcatt
     2341 cgagttccgc atccttcaga tcgtcagggc gaggcacccg ttcgatgcga gccgagatat
     2401 ggtagctggc cttgtcggca tcaaaacgtt cgagagcgaa gctcacaatc cgcctgaaaa
     2461 ggtcggagtt cactctggcg atgactcgaa gagcctcgag atagctcgta ccggcggtct
     2521 tgagatggat caagccctcg gagagcctcg cggcgatcgg atacaccgaa aacttgtcgc
     2581 ttcccgagtg caggctgagc ttgtagggcc caagcgtccg ggcgatagcc gcatgcgccg
     2641 cgtatgactg ctcaaaagcc ttcaaatcac ccctgtaatc gacgcctttc tcgaaagccc
     2701 cgatgaatcg gggcgccaga ctcacccacc ggacgccaag tcgcttgagc tccgccgcca
     2761 ccagatagtg ttccgcttcc gatgtcggtg catccgtctc gtcgacggac acctccagct
     2821 cgaaccggcc gatcccaagt ctggcaacca gatgccggta catcgcagcc gtgtgcgcga
     2881 tcgcacggcc atatttcacg gccgcacgca acaacgtttc ctcggtgaaa cgaacgtcac
     2941 ctcgacccac caggggaaat cgccggttga ggtaccgccg tcgcaggtct tcctcgctgg
     3001 tctccaggtc ggcccagggg agggcgttcg cggccattga agcggcggcc gcggacatcg
     3061 tatcggcttc gctgcagaca tgctcgccgg gatcgatcgt gaacatcgtg aaacccgctg
     3121 ccgcggtcgc gtcgatatcc gcagtcgttt tgagatggtc cgcatcactc ccgaaacctc
     3181 ctctccagcc ggcctgaaaa actccccaga ccgcgtcgtc catgacctcc tgcggcgttc
     3241 tttgcgtgcg ggtcatctcg cgaatggact gctgtgccgg aaaaggtaca caaccctggt
     3301 cctgcaccgc caggaggtgg ccgggcgtcg ccaatccaag ccggtcgccg agaccgaccg
     3361 atgttgccgg gccgcacagc cgcggcgagg tccagggaac cgcctcccgc aacgcagacg
     3421 ccgcatcggg gccggccgac acgaccgtca cctgcacgtc tcccgcactc ctcctgtgtt
     3481 cgggagacaa cgagccgaag gctccgagct tgcctctcct gcaggccagt atcaatcggc
     3541 gcgagcgccc ctccttgccg agggccacaa cgatggttcc ggttgactct accgacgccg
     3601 ggtaaaccgc aaacccgcag gttcctccga gttcgcctgc tgcctgctcc tgaagtgtga
     3661 cagaaagcgg ctcaccatcc agcagtcggc tgattgcgtt cattgattct tctggcagcg
     3721 aggtctattc ccagttcact cgcggggcat cgccccaaag aagctcgagg tcgtagtact
     3781 tccgcttctc ggcgtcgaaa agatggatga cgacatccac atagtccgca agaagccacg
     3841 tggccgtgtc gtagccgctg acactatacg gcttctctcc gatcgtcctc gcatattctt
     3901 caatatcgtc gcaggtcgcc cgcatctggc ggtccgaggt gccggtaccg atgacaaaga
     3961 aatccgcaac cgaagacagc cctctcaggt ccagaatcac aatgtcctcg gtgtggttgt
     4021 ccgaggcaat acgggctgcc tccttcgcaa aggccagcgc cttgttcttc ggctttgccg
     4081 tcctggtctt ggctttactc atcttgccgg atcatgttcc tcaacaccgg acaaaaagca
     4141 aggaggtctg cccgccaaac caagggcaaa cctcccgaat tcgtcgccca accagcggtt
     4201 ccggattcta atgttccggg aaacgcagac tcacagaccg gccaagagtt cacataactg
     4261 tttgcccagc gacgagctga tccgtgccag ggcccagata ctggctgcat tcttgcccaa
     4321 ctccacctgc gaaagctgcg aagtagtcag ttccgcccgc tctgaaagct gacgaagagt
     4381 caggctctgc ttgagcctct cctgacggat gcgctggccg ataagccggt taagctcctg
     4441 ctcgttcgtg taaaccaagc ccatctcctg cgcgattcgc tcaaccgcct tgagcaggtc
     4501 ttcctccttg aacggcttgc acaggtagtc acggctgccc agccgcatgg tctcggccgc
     4561 tgtctgaatg tctggatagg ctgtcatgac aatcggggca atacgcggat cctcagcctt
     4621 gattttctca actatttcta ggccattcag gtccggcatt ttgatgtcaa tgatcgccat
     4681 gtcgaacggg gctttccgaa cggcctgcaa agcctgcctc ggttgagagt aagactgcac
     4741 atgatagccg tgatccttca agctcgtgct gatcatcttg cagacctgtg gatcgtcgtc
     4801 caccacgagc actttgatat gatgtcttac agccatgtta acaagcacct tcagttggac
     4861 ttgactctcg ttgaactcca gattcaactt ccattattcc attgtagcga atccggtttt
     4921 ttctgctagt gccttctccg gcggaaactc cgtccggccg gttggccgaa aggcctataa
     4981 gccaagggat atttgaggtt taggaattaa cctaagtaat taatggataa taccttctat
     5041 ccaaacattt ggtcagaacc tccttgcatt aatcgaccac ggaatgagta gttttgccga
     5101 cctcaatgaa ttcgggtgtt tacccccttt gcttttttgt cgctctcgcg agcgcgaaac
     5161 cgcctgttgc accaactcgg cgggccgtgg ttgcacgggg tcgcttcagg ttttgattcg
     5221 aggcggactg tgaagtggtt gccccattcg gtcctcgaca gcccgttggt gtgcagatga
     5281 tcacacggga gcggcgaaaa ggtcgatcat gcccggtggg ttgagtcatc ggaaaatcct
     5341 ggggggtctt attgggtgtt ccgtggcagc aggagtgagg ggtgattcag gccttggagg
     5401 ttgccggggg cgccgacgac cgcactgggc agaggtctgc ggtggaccgc ggggcgctca
     5461 ccttgtcgac ttcggcagaa ttgtcagcac gcccccgagt ccttatcctg acctccttga
     5521 gttcgggttt tcaggaggtt gccacatggc tcgcaaattg agttcgattg ccccagactg
     5581 gtgggactac acgacgctgc ccgaagaact gcttgaggag gtggctcgac tgaccgtcga
     5641 cgacgtgagc aggctgtcgc gagacggctt ccgtgtcgtg atgtacgaca cgctggagga
     5701 gttctatctg gcagaagccc tcgaatacat cgaggcgtgg cggcaggcca ccgagagcca
     5761 gccggttggc atctgcggtc ccatcggccc cacggaacag cttcccttgg tggctcgtct
     5821 cgtcaatgcc ctcgaactga atctcaagca tgctcacttc tggggcatgg atgagtggta
     5881 caaggacggc cgagaagtgc ccgaaacgca ccctttatca tttgccaagg ccgatcggga
     5941 actgtgcttc aatcgtatac gtcccgacct gcggatgccg gagtcgaaca tccatttccc
     6001 caaggccgat ccgaccgctt acatcaagag ctgggaaaag gtgcgatgtg cggtcatgca
     6061 aggcggccaa ggcgagatca agcactgggc tttcaatgat ccacccaagc gcgaagcgcc
     6121 gtatgtcgat gagccgccgc cgcccgaggt gtatcgaaag ctcacgacgc gggtcgtgga
     6181 tctgcacccc gccaccctca tgcagaatgc tcgaacctcc ggtggcggca atgtggccgg
     6241 tgtcccgacg aaggccatca gcgtcggccc ggttgagaca tggaaagccg gccgcgtgtc
     6301 catctggcag gctggcaccc acgacaatcc cttcgggcaa cgccttacgg cctttatgat
     6361 ttcgaaggaa atcgtcgaca cctcggttcc gatgtccctg ctcgcccagc acccgaacgt
     6421 ccagttcaat tactaccgca agggccttgg aacctgcgaa gcggagatgc actgagcttc
     6481 gacgttacag acggcaacgc ggacgggttg ctgatttcaa tttgcggatc tcgggcaagg
     6541 tactgcagac cttgagtccc cggtttgcgg tcccgaatct cggatttgag tctggtgact
     6601 cccagccttg ttcgagcggc tcgccccgat tcccagagag gcttcgacgg gccgttgggc
     6661 agcagaaggc acgagtccaa gcccgaaagg tgccctgcga tttcattctg aactcgttga
     6721 tctgttcgag cgtaggagaa ggagaataga acctttcagc cggacatctg tgcggctcgc
     6781 gcgcatgcgt ctggatggca gtgggcttgg gttgcggccc gcaaaaaggc ggtccgcctt
     6841 aggattcctg gactgttccg cgccggcggg gcatctgtgg tatgagtgag aggcacggtc
     6901 atctgcttga agccatcctt ttggccgcga ttggcctgat tcttgcatat ttcctcggcg
     6961 tgcgcctcgg ctggctgccg gtgttctggg gtgcgacctt tctgctcatc gctgtcatgc
     7021 ttctgctcgc caacctgttc tggtgggcgt gggtcgatcg acagcttcaa ggtcgcggcc
     7081 gccgtaccgg ttctacgacc cgccgacgtc tcctgcggtc gggctgggct gcattcatga
     7141 ccctgatgct gtcgcctctg cttctcgtgt tcgtcggcct gcgggggttc tgggatgcgt
     7201 accccttcgt tctcatcgca ctgatcatgg tctggcacct cctcatggtt ctcctggtga
     7261 ccgtatgggc gccgttggcc ggtttctggg caatgttccg acgagtgaga gcccgagtcg
     7321 caggttctca ttcattcgaa aaaccggtgg acccgtcgcg gcgacaattg ctcctcgggg
     7381 ctgtgggtgt cgcccccgtc atcgtagccg gcgggtctct gggcttggcc gtatggcagg
     7441 gggggcggtt caaggtgcgc cgtgtttccc tgcgaatgcc gcggttgccg gatcggctgc
     7501 ggggtctgac gatcacgcat atcagcgacc tgcacgtcgg tcggttgttt cggcccgagc
     7561 acttgccgcg gctgatcgac gccgtcaatc aactcaagag cgatctcatc gccgtcaccg
     7621 gcgacctcgt ggatcattcc gttgacttca tgcccctcac ctgtgatgcc cttgctcaaa
     7681 tggagtcgcg atgcgggcgg ttcatcgtgg tcggcaatca cgatcttatt gattctcccg
     7741 aaaaagcagt gcaggagctt catcggcggg aaccgtttct cctgctcgac cgcatgctcc
     7801 cgctgctgat cgacggcgag accatccaga tcgccggctt gttctggtct cgctatgacg
     7861 aaccgagggg cgaagatcca ggtcacacag gtcgagttac cagaaccctg gccggtgcgg
     7921 attcagaccg cttcaccatt gctctggccc accatccgca cgcatttgac ccgctggccg
     7981 accgtggcgt ggacctgacg ttggccggtc acacccacgg ggggcaattg atgctcacgc
     8041 cgccgggatc gcacacgccg atcggaggcg gcagcctgct gttccgctat atctgggggg
     8101 agtaccggcg aggcgattgt gtcctggagg tcagcgcggg ggtcggcaac tggttcccgg
     8161 tccggatcaa tgctccggcg gagatcacgc acatccgact gatctgaggg gtaacggccc
     8221 gcgcggcggc gtgggccggc aatcacgctc tactcgcgga cgcggttcat catcaacaga
     8281 ttgcctatcc atggggatgc caggaaactg attgcgaaac cgactgcgag gctgttgacc
     8341 aggcctttga tcgcttctgc ggaagcccca gaatcgcgcg tccggctcca cgcgtataag
     8401 gcaaccgcgc ccaggccggt cagggccatg acggccgtat agaaggccat agccgttctc
     8461 ggccagccgc gggagcagtc aaacgtgccc gccagcggca ttgacagcag gccaaatatg
     8521 aacgaggcta cgagcaggct attgctgcgc aagccgaacc cgagcccaag gcaggcgact
     8581 gccagcaaga ggctgccccc aacccagttg gaagcaacaa tctgctctcg cgtcagggcg
     8641 agtcgtccga aacggttcaa gcgcaggagc aggttaaaca gcgggccggc cgtccacgtt
     8701 agaaagacga aaacgaggta gagaatcagg aagggggcga tgaacggtgc cagcggcggg
     8761 ttgctgcggg cgacgctgct gagggccctg gccccaaagt acccgcccag gatcagcccc
     8821 caacgagcct tgccggacaa cgagcccatc cacaagaaat aacggagcat cagcccataa
     8881 acgatgttct tggccttcag agactccacg atcccctggc gggcccactc gttgccaggt
     8941 tcgagcctta gaaccttacg gccagatatc tgtgcggctt gcgcgcatgt ttctggatcg
     9001 cagaacgctt gggttgcggt ccgcaaaaag gcggtccgcc ttagggcttc acggaagctc
     9061 tccaacgcct ggcgatgctt gccctcgtgc aggaaggccc atcctcgatt ggcatgggtg
     9121 ttcgcgtcat cgggatcgcg ggccagggcg gcatcgagtg cctgggcggc ctcgagcctg
     9181 cggcccaatt tgaccagcgc cgccgcccgc acattggcgg cctgcacatg ctccgggtcg
     9241 agtcgcagac cctcttccgc cgcggtcaat gcttctcgcc acagccgttg cgtcaagcgg
     9301 atctgaccga gaagtgcgtg gtagtaggcg ttctcggggt ccagcctgat ggcctcgtga
     9361 atggcctcca gagcttcctc gggacggttc ctgtcgtgca ggatcgctgc ccggatgtag
     9421 tggggaaacg gctcgtcggg ggcggagtgg atggcggtct caacggccgc agttgcctcc
     9481 ttgagtttct cctgctgtgc gagactcagg gccagcaacg catgtccgcg cccgtcgtcg
     9541 gggctttcgg ccaaacctcg ccgggcctcc tgctcagcca attccggccg cgattgttcg
     9601 atcaggaccc acgctctatc gaaatgaatg ctcataacag cttgaggtat ttcagaacgt
     9661 cgtcgtagat cccgccctgg ttggcataga tggcatggtt tcgggcggtg gagaaccatt
     9721 ccttggtgga tggccgtacg gcggcgacgg cggccagaag gtctttggtg gtcatcggtt
     9781 tcgggacacc ggcccgcatg gcttcctgga gcttgcgttc gatcgcctgg tcgacgagag
     9841 ccttgagatc tgcccccgag aacccgttcg tcttggccgc caacttatcg aaatcgatct
     9901 cctcggcggg tttgcctcgc accagtatgc gaagaatcgc cgatcgggcc acagaatcgg
     9961 gtggcgggac aaaaatgatg cggtcgaaac gccctggccg gcggaatgcc gaatcgagat
    10021 gccacggggc gttggtggcc gccagaatca ggacgccgtc gttgctgtac tgggcgccgt
    10081 ccagttccgc gaggaactga ttgaccagat gacggccggc gctttgtcgc aggtcggccc
    10141 gactggctcc gagcgcatcc acctcgtcga agaagagcac gcacggcttg tgctgccggg
    10201 catgctggaa cacctcgtga aggttgcgct cgctgttgcc cagccacatg tccagaacgt
    10261 cattgatccc gatcgtcaga aacccggctt tgatctcgcc ggccgtcgcc cgagccagat
    10321 gcgtctttcc gcagccgggc ggaccgtaaa gaagaatgcc tccgccaatc tgcttgccgt
    10381 acgcttcgta cagctcgcgg tggctcaggg ggtggatgat tttcagccgg atttcctctt
    10441 tgaccgcctc catccctccg acgtcgtcga agttgatccg gggccgctcc aactctatgt
    10501 ccggaccgga tgaggattgc tcgccgggca cgcggacccg gccgtcgatc acctcggaat
    10561 cgggctcccc gttcagtccg agcgtttctt ccagggaatc gtcggctgcc tcgggatcct
    10621 gctcgatgcc tcgacggtac tgccggacag cgcgatcgat gtcaccggcc cgcaggagga
    10681 ggcgggcgtg cagcacatag acctcgcctg cggtggatgg gctcttgagc agttcctcga
    10741 caatcacgag ggcagggctg tatttgccgt cccggtagaa agcgttggcc agccccagct
    10801 tcagtcctgg atcgtcgggg caccgattga gcgtttcccg gtactcctcg acagcctgtt
    10861 gtgtgtggcc atatcgcaag agcatctccg ccaggtgctg ccggaggcga acgttgtccg
    10921 gtgcatcccg caacgcgtcg cgcaaggcct cgattgcatc atcccctgca gccatgacac
    10981 ccatctttcg ctgataaccc ggccgtcgaa cccggcagga atcacagttc tcagtcatca
    11041 ccactgacaa ctgagaacag gcgactgacc tctacttctt cgccttcccc tgagcggcca
    11101 ctgcggccat agcggctttc accttctccg ggtcgcccag gtagtagctc ttgatggtct
    11161 tgaggccctt gtccagctca tacaccaagg gcataccggt cggtatgttg agttcgacga
    11221 tgtccttatc gctgacgttg tccaggtatt tgaccagcgc ccgcaagctg ttgccgtggg
    11281 cggcgatcaa cacccgtttg cccagcttca ccgtcggggc gatgacgtcg tgccagtagg
    11341 gcaggaagcg cgccactgtg tctttcaggc actcggtcag cggcagcgcg ccctcggtca
    11401 ggtccgcgta gcgggggtcg ttgcccggat accgcggatc cgttttctcc agtggcggcg
    11461 gggggatgtc atatgaacgc cgccagatct ttacctggtc ctcgccgaac ttcgcagcgg
    11521 tctcggcctt gttcaaaccc tgcagcgccc cgtaatgccg ctcgttaagc cgccatgatc
    11581 ggtacaccgg gatccacatc aggtccatct tgtcaagcgc gatccacagc gtgcgaatcg
    11641 cccgcttgag caccgaggtg taggccacgt cgaaggtgta gccctctttc atgagcaact
    11701 cgccggcctc ggcggcctcc ttgatcccga cctccgacag gtcaacgtcg gtccagcccg
    11761 tgaaacggtt ctgcttgttc cagtcacttt cgccgtgtcg cagtaatacg actttgtaca
    11821 tcgatcgtct ccatgagcta tcaattgccg ccacgtttgc ggcgtcatcc acgaagtcgc
    11881 atattctacc gattttggct ctattgccaa atggggacgg caacgcgcgg cgccgtcaga
    11941 tgcgagggcc gtcgaacagt ctcaggtcgg gcagcgcaga cgcgataacg acaaaatgcg
    12001 catcgtatgt ccatagcagc aagcccccgc ccattgccac ggtggcaatc agggcatcgg
    12061 caaagggtac tgccaggcct cgtgaccgca aggtgcgaag gatacgcccc aggtcattcc
    12121 acaccttctc gtcgatcggg agccggacaa aacagtccaa agcggcggtc agttctggta
    12181 gatcaggtac ttctgtcgca cctccctgaa gcggctgaga gagccgcgcc agccggccgg
    12241 cagcgtcgtc ggggtttcag cggtcttcag gttattcagc gtttccgcgt tggccagcag
    12301 gttgatcacc ttgtcaacct cgaacaccgg gccgaagagc ttcttcaggt gccaggcgat
    12361 cgtcaagccc gactcgacca tgcggatgtt ctggcggtcc gtgaccagca cgtacactcc
    12421 ttggcatttc tccttcgcaa acttgctcga tgtgggagtg aactcaatgg ggatgaaccg
    12481 tagccccggc aggccggcct cgttcagcga agccgccagc ttacggccgt cgatccaagg
    12541 ggcgccaaag atctcgaacg gctgatccgt gccacggccg acggaaacat tggttgcctc
    12601 caacaggcag atggcagggt agagcgtcgc ctccgtcaga ttccgcatgt tcggcgaggg
    12661 gttgatccac atcaggccgg tttcgtccca ccacatcgat cgacgccagc cctgaagctc
    12721 taccaccgtc aggtcgcaat tgatgtcgta ctcgacgttg cacatgcggg ccagctcacc
    12781 gaaggtcatg ccgtggacca ccggcagcgg accgtaggcg gtgaaaccgt aacgattctt
    12841 gtcggcaata ggaccgtcca caatcaggcc ggtgatcggg ttcggacgat cgagcaccac
    12901 cattttgagc ttgtgcttgg cagccgcttc aagggcgtag ccaagcgtcg ccgaataggt
    12961 gtagaacctc gccccgatgt cctgaatgtc gtagaccagc gcgtccacgc cttccagcat
    13021 ctccggcgtg gggcgccttg tctcaccgta aaggctgtac accttgaggc cggtcgcctg
    13081 gtcgaccgtg tggcccactt tctcatccaa tgtgccgtag agcccgtgtt ccggcgaaaa
    13141 caactttacg agtttcacct ccggcgcctt gaacagcagg tcgactgttc ggttgccctc
    13201 gcgatcgcga ccggtgtggt ttgtgatcag ggcaaccttc cttccggcca acagcttgaa
    13261 accatcacgc ttgaggatgt cgatgccgca gagcacctcc gccggcggac caaacgcttt
    13321 accggtgatc gcagccgcca cggcggttgc cacctgccgc cgcaggtcgg tgaccgtgcc
    13381 cttatcgtcg ggatggacgc ggttggtcag caggatcacg taacagtcat gagtcggatc
    13441 gatccagtag ctcgtgcccg tgtaaccggt gtgaccaaaa gtcgtgcccg cctggaagag
    13501 gtcgccccgg gccgacgaat agctggtact catgtcgaag ccgtatccac ggcatcctgt
    13561 ccccccgggc aggcatcgct gcgaggtcat ggcttcgacg gtcatttgcg aaaggattct
    13621 cttgtcgtca agcaccccct tgtgcaggat cattcggcac caacgtgcca gatcctcggc
    13681 cgtgctgaac agcccggcgt gccccgcgac gccgccaagg gcccatgccc gcgggtcgtg
    13741 cacctggccc tgcatccatt tgccgtcgcg tttctcggtc ggggcacacc gagaccacag
    13801 gctctctggc gggcaataca tggtgtcctt catgcccaga ggaagaaaaa cctcctctcg
    13861 tgcgaagcgg tcaatcggtc ggccgtcgat gactcgtacc agttcgccca gcacgatata
    13921 gccgacgtcg ctgtagcgga aatcggtgcc cggcgggtag acgggcgcga ggttccagat
    13981 acgttcccac gccttgacag gcccgtcagc gtaatccttc atgggattgt caggaacaaa
    14041 gcctcctcgg tgcaacagta gctgttcgac cgtcaccttc tccttgccgt tggccccgaa
    14101 ggcgggaatg tgtttcgcca ccgagtccgc cagaccgagc ttgccacgtt ctacgagaat
    14161 catgatcgat gtggcgcacg ccacggtctt tgtgaccgaa gccatgtcga agatcgtgtc
    14221 ttttgtcatg ggaatgggct gtgggtcgga cgtccgtaga ccataggctt tgaaatagac
    14281 ggtcctgtcg ccttgtccaa ccagcaggac ccccccggga agttcttttc gcccaatagc
    14341 ttcttcaatc agtcgatcgg cacgctgcat ccgttccatg tcaaacgata cctggccggt
    14401 ggacgttgcc gctgcagtta ccgtaagaac actcacagtc gcaagccaat ggccgcaaaa
    14461 catgtttccc tgcctttctg caagcacggc gcgaaatcac aaccacgcaa acgccagggc
    14521 ggcgctatgg tactcgcaag tcggcgggca atcgacccgt gctccggcca tgcgcctatt
    14581 cgtcgatgat ggatcgcccc gcgtggctcg tcagcagcgc gaggatgatc tccgtggcct
    14641 ggtagacctg ggccaactcg acggattcgt cggccgtgtg agcctgggcg atatcacccg
    14701 gaccgaaaag cagcgaggcg atacccgctt cgctgaacgg tccggtgtcg gcaaagtaat
    14761 tcactccacg tggggcgctt tgtccgttgg cctctcggca cacagtcagc agcttccggg
    14821 gcaacggctt gtctgccgga caatccagcg gggggtgtgt ccgccggctc aggatgctga
    14881 acttctcccg accgcccaga taatccgaca gcatccgcgt gaaatcagcg acgatacggt
    14941 ccgccggttc gccgggaatg aatcgcccgt cgatctgggc gcggcaactg gacgggatca
    15001 cattgatctt gctcccgccg ttgatggtgg tcaccacgca ggtcgaggtc ccgagaaggg
    15061 ggtgagaccg acggccgatg tgccttttcc aggggtcccc gacgaaatca accactctgg
    15121 ccatcaactc gatcgcgttt gtaccttgag tcggtagtga ggcgtgacac gaacgaccgt
    15181 gcgtttcgat ctccagccac agggggctct tatgggcggt cacgacctcg cagttcgtgg
    15241 gctcgccgac gatggccgcg tctgcggtga agcccgacct caccagagcc gttgcgccgt
    15301 cacagcccgt ttcttcgctc accgtggcca cgaagcacag ccggtcgctg ggcaaccgtc
    15361 ccgcttcatg tgccagggcc aaggcggtca acaggctggc gagcgatcct ttcgtgtcgc
    15421 acgtgccgcg gccgtacatg cgtcccccgc gaatctcagc cgcgaacgga tctacggtca
    15481 ttccctcggt cggcaccgtg tccatgtggg cggagagaac aagccgcttg ccggttcctt
    15541 gacccggcca gtaggcaatg agattgggac gtcccggaaa gacctcttgt cgttcgacct
    15601 gcattcccat ccgggacaaa tggtcggtca ggtattccgc catccgttcc tcagcccgat
    15661 cgcgattggc ctgctcaggt gtcgagacaa cgcttggaat ccgcaccagg tcggccagca
    15721 gtcgacatgc acgttgttgc tcttctctgc taagctgtcg catgaattgc agatccgtcc
    15781 gtctctctac accacttcga tgaattccag atcgtcggtc ctcagatcca gcagcgccac
    15841 cgttggcacg cggactcgat gaagggcgcc cggattgatg attcgcatgc caccgctgcg
    15901 gctgtcgtgt cgccggtgcg tatgcccgaa aagcaggtag tcgcggtctg cccgctcata
    15961 ggccttgacg aattcctttt cgtttccatg aaacacgccg attcgcttgc cctcgagccg
    16021 caactccagg gggccatttg gccaaggtaa acccattgac tcgacctggg cccgccacgt
    16081 cgggtcgggg cagtccgtat tgccccacac gaaccagcat cgacgaccga caagctcttc
    16141 cagaacgttc aggccaccga tgtccccgca caagataaaa gcctcagcac ctcgggcatc
    16201 gagcaatacc aaggcgcgat gaaccattgc tgcgttgccg tgggggtcag acaaaatgcc
    16261 gagcctcatt cccggtttcg cctccgagga ggaccttcta ccgaccggtc atactgattc
    16321 cggtcgcgcg cgctctctga gggcacccca ttatggccat cctgtcccct ttgaacagtc
    16381 ccgcaggatg aacccggctg gcccaagtgt catgcttgtg ggcatttgcg tcattggccg
    16441 tctgtgttta gcataagtcg gctggaggat gtgccttcgg gcaggatccg gatagaccca
    16501 cggccggctg ccggcctgcg agatgacggg atacagacgg atgtttgctg ttattacgga
    16561 tatacattcc aacattgagg cacttgaggt cgtcctcgcc gatatcgacc gccgaggcat
    16621 caagacaatt gtatgtctag gcgacatcgt aggatatggc gcaaacccca aggagtgtat
    16681 cgatctgatc atcgagcggg cccagtattg catctgcggc aatcacgatg cggccgtctt
    16741 ctacgaaccc ttcaacttca acatcggtgc cgagcgggct tgctactgga cgcgacaggt
    16801 gcttgaggac gagccgaata aggccatccg agaccggcga tgggagttcc tcggaaagtt
    16861 gccccctcgc ctggtctatg agggaatgct cttcgtccat gggtcgcccc ggcgacccgt
    16921 gaacgagtac ctctttggtg acgacgtcta ctccaacccg gccaaactga tgtccaattt
    16981 cgagcggttc aatgaggtgg cttgcttcgt cggtcacacc cacgtgccgg gcgtttttgt
    17041 ggacgatcct tatttcgagt cgccgggcga attggcggaa cccggtgttt atgagatcgc
    17101 tgaagacgac aaggtgatca tcaacgtcgg gagcctgggg cagcctcgcg atcgcgatcc
    17161 gcgggcagct tacggcatcg tggatgacgg gcgggtggag tttgttcggc tcgaatacga
    17221 cgttgagaag accgtgcaca agatcctgag cacgcccgat ttggatgatt tccttggaca
    17281 ccgcctgctg gaagggcgat gatccggccg tgtgggcggc ggatcccgtg ttggatgccg
    17341 ctggcccaag cggtcggtga agtggctggt tcgctcgctg ccgccgcgcc cggcttcatt
    17401 gcctccatac catagccgga aaggaaacga tggaaacgcg acgaatgatc tccgctatgc
    17461 tggccgccat agcggtcttc tatgcctggt tgctcctggc caggatgatc tggccgcagc
    17521 cgacacaacc cgcgacgcag cccgcgacgg cggtcaacga ggccacgagc cggc
//
LOCUS       DAOTPW010000115        17497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3248_length_17497_cov_6.485380, whole genome shotgun sequence.
ACCESSION   DAOTPW010000115 DAOTPW010000000
VERSION     DAOTPW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17497
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_17497_cov_6.485380"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(544..1833)
                     /locus_tag="P5316_11775"
     CDS             complement(544..1833)
                     /locus_tag="P5316_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HRR85677.1"
                     /translation="MSTEPTIICPNCRTEIRLTESLAAPLIESTRREFEQRLARKDQE
                     IAKREQAIKERESALAREKESVDQQVLEKLKQERTRIAAEEARKAKLALANDLDQKAR
                     ELADLQEILKQRDQKLAEAQKAQAELIRKQRELDDAQREMELTIEKRVQESLDKTREQ
                     AKREAEEQLRLKVMEKEQTIASMQKQIEELKRKAEQGSQQLQGEVQELQLESLLAARF
                     PQDVFEPVPKGEHGGDLLQRVVAPTVGRCGTILWESKRTKAWSEGWLPKLREDQRAAQ
                     AEIAIIVSQAMPKGIESFDMIDGVWVTHPRTAIPVAMAVRQTLVEVAAARQAGEGQQT
                     KMELIYQYLTGPRFRLRVQAIVEAFSTMQEDLEKERRAITKQWEKRREQLDRVMYATV
                     GMYGDLQGIAGKTLQEIEGLDLHLPEQDSSRGQPRLP"
     gene            complement(2066..4717)
                     /locus_tag="P5316_11780"
     CDS             complement(2066..4717)
                     /locus_tag="P5316_11780"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRR85678.1"
                     /translation="MKGDTLQRFLLSCCLPLCITGVSPSAADQAPAPQRPNIILILAD
                     DLGFSDLGCYGSEIATPNLDALAAGGMRFTQFYNAARCCPSRAALLTGQYPHAVGVGH
                     MCADYQLPGYQTGLSDRCVTIAEVLRQSGYHALMSGKWHVGMAPEHRPLVRGFEHYFG
                     ILSGACNYFRPEPHRMLMLDDRRIEPVDPSFYMTDAITERAVEYIDQYAGRDKPFFLY
                     VAYTAPHAPLHARPEDIARYRGTYRAGWDELRQRRYHRMIELGIIDSRWSLSPRDPGA
                     AAWDDVCDKDREDLKMSVFAAQIDRMDHGVGRIVEKVRVRGIEKNTLILFLSDNGGCA
                     ENLDEGKPGAPIGTIDSSIGYGPPWANLSDTPFRRFKRMAHEGGIATPLIAYWPSVIT
                     RGGEITHEVGHVIDLLPTVLDAAGAEYPKFFQGRDIRPADGLSLLPVLRGHQREGHEL
                     LAWEHEGHRAVRCGRWKLVSYFPDPWELYDLELDRTELHNLADRMPDKVRELADRYEA
                     WARRCNVVPWETISRRARSKADKTYLEQGRPKEPVVIEPGEPQLFVDDWILDSSLSLM
                     RMLHRPVKDNGGQTPVIAPPEGERALVAKGSILFDPRLKSYVMFCEALPSTKIHRYIS
                     PDGLTWSDSGPIELDGSSDIRRLDRVDPSPSAAEADGSVPGDESYACTAWRYGPLWLG
                     ESVVWHGRDDCPWSAAGCAAIKLISSRDGLNWHKVVFEDLSGTPEVFIPAGRQGGNNG
                     RNDGGCLSLFSQGPLRIGDELVFYYGASSYGKNASQPDGVSGGGIFRARLRPDGFVSV
                     TGLALTTRPLQFKGRELIVNSNGPIIVEALSPEGQVEGTAFLATDGIAHLVRFAGRSL
                     ADVLPDNHGRLRFQVEQGAHLYSFTIR"
     gene            complement(4827..7637)
                     /locus_tag="P5316_11785"
     CDS             complement(4827..7637)
                     /locus_tag="P5316_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1961 family protein"
                     /protein_id="HRR85679.1"
                     /translation="MQTIALSLKMPLVRCCKRIAASRGRDSRRAGLLVVCVATGLSAL
                     SCASPSRRADLVTPTARPNFVLILVDDLGWAELGCYGNRFNETPHLDRLANRGVQFTN
                     AYAAAPVCSPTRAALMTGQYPARVGITDYLRADDERFLSPEYHTLAEALRDAGYATGL
                     LGKWHLTGDYRLKRGEPSLHGFDEVICSETRYIGAGSYFAPYLFMPEVTGPRGEYLTD
                     RLNREAVDFIRRHKDRPFFLYLSHYSVHTVLAAKPEMIEHFAAKRGVGKTRNNPVLAA
                     MLASIDEGVGNITSALDELGIADRTVLIFASDNGGERRVTSNAPLRGGKSQLYEGGIR
                     VPLIIYRPGSRRAGKTCNVPVITPDLYPTLIEMAGLKNRPNRMIDGESIVGLLDGDRT
                     LQRDSLYWHYPLDRPHFLGGMSAGAIRQGNYKLIEFFDNGKVELYDLKTDLRERENLA
                     HRMPEKAAQLRAQLARWRASVGAVVPNSAPATAPSTFQGKTRPSGQAPAPSPRPHLFS
                     PPSPGDSRAPDGSPATSQSFSATLPASLLDDRGTLVMWFKPDKLLRGAGPTLSLLESD
                     AVNVQLLCTDPSVTLDTRWGSVMTGTPQTGESRPYVFQTLLTHLKADRWYLATWTWNT
                     ADASRNEFFLDGVRQENASPFNYPGQFRAAGRDVTLTVGGPGLIVSAVGLYEEPISEE
                     SIKAHCEAAGHTPYTDEGVRFTGEKFIPVDVDWEHPLYSTSFEDPSELKDWRLEGGRS
                     MTAANGKLVLESNGESTRSESAANHLVCWLDREVPADFLLEFAVRPENRNRGLNIVFF
                     NARGCNGENIFEPPIKPRNGTFSQYHSGDLNNYHISYWSGGRGTSNLRKNRGFRLAAI
                     GKDLVTPAPQDAFQVVRIYKRGGQIRLTIDDVLSLSYDDDGKTYGPIHAHSGWIGLRQ
                     MAHTQRCEYDYVRIYVLKTPSP"
     gene            complement(7802..9316)
                     /locus_tag="P5316_11790"
     CDS             complement(7802..9316)
                     /locus_tag="P5316_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR85680.1"
                     /translation="MSHHLTTVAYTLWTCGIVLQSACSQLAGTGNGIPDAAADKAVGA
                     QRPADAGATRRPNVLLIISDDLNNSLGCYGCPVAITPNIDRLAAQGVRFDRAYCQYPL
                     CNPSRASFMTGRRPDTTGVLENRTHFRENLPDVLTLPQLFQQVGYFAARVGKIYHYGV
                     PGQIGTDGLDDAPSWNLRINPRGRDKDDEDEVIQYTGPKGSLGAAISFLAAEGTDEEQ
                     TDGKITGEVIRLMEENKNRPFFIACGFFRPHVPCIAPKAYFRQHPLPTLSLPQEPADH
                     LALIPEAALQVRPPNYGLTEGQLKNFLQAYHACVSFVDAQVGRLMDALVRFELADNTV
                     VLLLGDHGWLLGEHGQWQKMSLFEESARVPLIIAAPGTKGKGKACPRTVELVDVYPTL
                     AALCGIDPKGAEGASLVPLLNDPQASWSKPALTQVSRGTRLGTTAAAQSGQRGFMGRS
                     LRTERWRYTEWDEGRQGTELYDHQNDPKEYRNLANDPAYADMAAKLRQMLRSRS"
     gene            10294..11709
                     /locus_tag="P5316_11795"
     CDS             10294..11709
                     /locus_tag="P5316_11795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRR85681.1"
                     /translation="MDNAYSSSESSRTATTARPRWRTLVRSLRFKASVLVILILAVVT
                     SISSTLTTQVLGQVFYENEYARTREWAQSLAISTAEAIQARDAESLGLTVQDLIKSNS
                     VAYVVFSDAAGEVLVSGEVKAGLLQSVVSADGQTLSLKTMNVPRLVWNKELGLACSEV
                     SVPVLSKSTIRPTGTSGRAIVGYLHLAVDVTATKKQLELVNRRVRQIAMVMLLLTVPC
                     SVLVARRVVAPLKELSETACAIARGSTDARAHINTEDEIGDLAQSFNTMADRLTKSQM
                     ELLELNAELEERVEQRTRELQELAARDPLTGLYNRRHFSEVIAREYAAAERYDADLTC
                     LMVDLDRFKETNDRFGHRTGDEVLAIMAHVISTELRGSDVAARFGGDEYIILLPQTSA
                     ASATVLVDRITNRFKEEVTRKFPDAPVGVSIGVASLRTTRAASAEALIHEADVALYSV
                     KASRKSGALRDKRPRPARSRT"
     gene            complement(11745..12296)
                     /locus_tag="P5316_11800"
     CDS             complement(11745..12296)
                     /locus_tag="P5316_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6141 family protein"
                     /protein_id="HRR85682.1"
                     /translation="MPGSRSPEVIFTEVQQFRQVWLWAMVLAWPASMAIILGYVLFRQ
                     LLLASPAGHLTWNSIALIAAFAVPLLVTLIFAWLLYATRLITEVRGDGLYVRFWPLGW
                     RHIRFDEITECWPRTYRPIREFGGWGIRWGRGGKAYNISGNRGVQLVLAGGRRLLIGS
                     QHAEELAAAIRDALICSTYKESE"
     gene            12801..14426
                     /locus_tag="P5316_11805"
     CDS             12801..14426
                     /locus_tag="P5316_11805"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85683.1"
                     /translation="MTRQQTETERALCSRRLLLASAILLSIPTGRLVAADSAYLYGIH
                     WWGYTPGQAVDTGPAAMLDCPAFGGWDVETIVTHSANWWQASFFTGLYQDLYTNKNVT
                     VITRIDYNWGETVPSPTNPDYASWPTSVVNTVNTLRNYCHIWLIGNEPNIVSEGNSWP
                     DNHVTPAGYAAIYRNVRNAIHSSAQASPAGEHVVLIAAPSPGGVIAGVRWMDGNQWLG
                     QVIDNIPAAEIDGIALHAYGGSLTDFHNSYVSQLNLIDGKGLQDRPVYITEWNKVSTE
                     PVIAQFVRDCYADLNTWNQTPGKHNIRCLCWFIYDWGQQNTNPDFQNMSIEYWKNNGE
                     PAGSPNDLYTAFQQTVDLRYPAGLVGTRPMGRSIGRSPESFAREVYYRDELANDTFEV
                     WNAGTDTLNYAITDDATWLNVNPTAGSSTGEHDTITILYDTEHLAIGSYLATIAISDP
                     AASPPTAIVTVSLSVVDSPYAPADFNRDRFVDETDLDLFLDCLTGPNAGPPATGCKRT
                     DIDWDGDVDQDDFGLLQRCISGTGSPADPDCAD"
     gene            complement(14436..15815)
                     /locus_tag="P5316_11810"
     CDS             complement(14436..15815)
                     /locus_tag="P5316_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85684.1"
                     /translation="MRISIAAGLVGVFLAVSPAMGQSLPADDGRIIALGTNDPDVQTK
                     ALHDLVAVGAPAVPTLIRLLGRTAENPTAGKMAYETLFRMVQVCAGTNNGGDVERALV
                     RELKANHPIETRRHICALLGFIAAENASVDALYIALNTPELAETARQSLARIPNRRAG
                     QCLMGAVQITTGETRLGVIASLALRGDPAAASYIAGGAIEGDPATARAVMDALAMLPS
                     AESFDVLAQAFKSGRPGSASALIRLMETLMDAEMFQPAEYVFKILPDASSLSVPERCR
                     LVHACGRLATKEAVGIVFGGLKDDNVRVRAAAVQACELLPGNDLTTAVAEQMTQAAGP
                     LKADLIEILGRRGRWMNDTAAGTIIDAIGDADESVRLAAIRAIRDAGISAGVRELVRL
                     LSGPAGPTRDAAENALHHMRGQASTAAIAKLLDEAAPDVRTKLQAALARRGNSSVVPT
                     TSRTTNDNN"
     gene            complement(15867..17174)
                     /locus_tag="P5316_11815"
     CDS             complement(15867..17174)
                     /locus_tag="P5316_11815"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85685.1"
                     /translation="MSEHLPISRRRLLGQGARILTATAMSPYVLTGRARGGAETRPAN
                     DRIGMGFIGVGGMGRGHLGNFLQFPDVQVVAIADVFDQSRNEAMKMVREAYGKDIPAY
                     NDYRELLARKDVDAVLIATPDHWHALTAIHACEAGKDVYCEKPLSYTIREARRMVDAA
                     RRNATVFQVGSQQRSCSNFRLACELVRSGRIGKVGLVRATIGGGPVCDWEPNVPPPPG
                     LDWNFWLGPAPWAEYTPKRCISDFRWFYDYSGGKMTDWGAHHNDIAQWGLGMDNSGPV
                     KTEPVSAAFPTRGLFETAVSFHVRHTYANGVVLETVSHGRNGVRFQGTDGWIEVGRGW
                     IEAGDPAILNDPLGPGDVHLYRSPGEDALGHERDFLNCVRTRKRPICDVEIGCRSVTV
                     CHLGNIAIRTGRTIQWDPEKEIIINDPALDRWLDKPYRAPWFA"
BASE COUNT         3216 a         5464 c         5489 g         3328 t
ORIGIN      
        1 gtcccagatg ggaatctggg accagcgggt cacagccccg ccaagtcgca tcgaccaagc
       61 agggcgtcga cctgccgcca ccttgaccgc tcccccgcgc cacacaaacc caacatcaag
      121 cgcaaccgca attcgccgcc gctccgctcc aaccgccccc tccggagcac cccccgtgtt
      181 ctccctcccg acaacaccgg cggagaccct gcgcgccgaa cgcacgagcc gacggacttc
      241 aatcgaatca atctcccggc ggcccaagga gagaagatga tgatcgatac aatctcgata
      301 accccgacac gcgcgacctg tatgctcaat atttacaagc ctttatgtgc aggagccgga
      361 cgtgcgcacc ccggccgacg gccaacggct cgtcgccgac cacgcaaaac gccaaatgaa
      421 cccaaccttc agcatcgctt caatcgcccg gccgcgcccg tgcccctcga tatccctaca
      481 ccgcccccga tacagactca ttcttccatc ctccgctctc ggcccgcctg cctccccgtc
      541 gtttcacggc agtcggggtt gtccccgcga tgagtcctgc tccggaagat gcagatcgag
      601 accctcgatc tcttgtagtg ttttaccagc tataccctgc aaatcaccgt acatcccaac
      661 tgtggcgtac atgacgcggt caagctgctc gcggcgtttt tcccactgct tggtgatcgc
      721 cctacgctcc ttctccaggt cttcctgcat ggtggaaaag gcctcgacga tggcctgcac
      781 gcggaggcga aagcgcgggc cggtcaaata ctggtaaatc agttccatct tggtttgctg
      841 tccctcgccg gcttggcgag cggcggcgac ctccacgaga gtctggcgca cggccatggc
      901 gaccggaatc gccgttcgcg ggtgcgtcac ccagacgccg tcaatcatgt caaacgactc
      961 gatgcccttg ggcatcgcct ggctcacaat aatggcgatc tcggcctggg cggcgcgttg
     1021 atcctcgcgc agcttgggca gccagccctc gctccaggcc ttggtccgct tggactccca
     1081 gaggatcgtg ccgcaccggc ccacggtggg cgcgacaacc cgctgaagaa gatcgccgcc
     1141 gtgttcgccc ttgggcaccg gctcaaagac gtcttgcgga aaccgggccg cgagaagaga
     1201 ttcgagctgc agttcctgga cctcgccttg aagctgttgc gagccctgtt cagccttgcg
     1261 cttcagctcc tcaatctgct tctgcatgga agcaatggtc tgctctttct ccatcacctt
     1321 gagcctcaac tgctcctcgg cctcgcgttt tgcctgttcg cgggtcttgt ccaatgattc
     1381 ctgaacgcgt ttttcgatgg tgagttccat ctcgcgctgg gcatcgtcca gctctcgctg
     1441 tttgcggatc agctcggctt gggccttctg ggcctcggcc agtttctggt cgcgctgctt
     1501 gaggatctcc tgaagatcgg caagttccct ggctttctga tccaggtcgt tcgccagggc
     1561 gagcttggcc ttcctggctt cttcggcggc gattctcgtt cgctcctgct tgagcttctc
     1621 caggacctgc tggtcgaccg attccttctc gcgggcgagg gcgctctcgc gctctttgat
     1681 ggcctgctcc cgcttggcga tttcctgatc tttgcgggcg agacgctgct cgaattcacg
     1741 gcgggtcgat tcgatcagcg gggcggccag agattcggta agcctgattt ccgttcggca
     1801 gttcggacag atgatggtcg gttccgtgct cattgcggct gctccttcct ccctgcgtcc
     1861 atcaggacgc gaaggccgtc gttgagcggg cagtctcgtg gtctgcaccg cgcatgatcg
     1921 gaggattcgg tcgcaggatt cgctctcatt ttcatgactc ctgaggccac gacaacgtga
     1981 ttatcgctgg aaggcattgc gaacgcgagg ggaagcgcgc cgacacgggt gttgccgcca
     2041 tttctccaac tgacgatcgg cggcgtcagc gaatggtgaa agagtacagg tgggcgcctt
     2101 gctcgacctg gaaacggagg cggccgtggt tgtcgggcag gacgtcggcc agcgagcggc
     2161 cggcaaaacg caccaggtga gcgatgccgt cggtcgccag gaaggcggtg ccttcgacct
     2221 ggccctcggg tgagagagcc tcgacgatga tcggtccgtt gctgttgacg atcagctcgc
     2281 ggcctttgaa ttgcagcggt cgggtggtca gggccagacc ggtgaccgag acaaagccgt
     2341 cgggacgcag ccgggcgcgg aagatgccgc cgccgctgac gccatcgggc tgggacgcgt
     2401 tcttcccgta cgacgaggcc ccgtaataga agaccagttc gtcgccgatg cgcagcggcc
     2461 cttggctgaa gaggctgaga cagccgccgt cgttgcggcc gttgttgccg ccctggcgac
     2521 cggcaggaat gaaaacttcg ggcgtgccgc tcagatcctc aaagactacc ttgtgccagt
     2581 tcaggccgtc gcggctgctg atgagtttga tggccgcaca gccggccgcc gaccagggac
     2641 aatcatcacg cccatgccag acgactgatt cgcccaacca cagcggcccg taacgccatg
     2701 cggtacaggc gtaggactcg tcgccgggca cgctcccatc ggcctccgcc gccgatggcg
     2761 acggatcaac gcggtccagt cgccgaatgt cagaggaccc atcgagttcg atcggaccgc
     2821 tatcgctcca ggtcagtccg tccggcgaga tgtaccgatg gatcttcgtc gagggtaggg
     2881 cctcgcagaa catcacatac gacttgagac gcgggtcgaa caggatcgag cccttggcaa
     2941 cgagggctct ctctccttcg ggcggcgcga tgaccggagt ctggccgccg ttgtccttga
     3001 ccggccggtg aagcatgcgc atgagactca aggacgaatc caggatccag tcgtcgacga
     3061 acaactgcgg ctcacccggt tcgatcacca ccggctcctt gggccggcct tgttcgagat
     3121 aagtcttgtc ggctttggag cgggcacggc gcgaaatggt ctcccagggc acaacgttgc
     3181 agcgccgggc ccatgcctcg tagcgatccg ccagttcgcg gaccttgtca ggcattctgt
     3241 cagccaggtt gtggagttcc gtgcggtcca gttccaggtc gtacaactcc cacgggtcgg
     3301 gaaagtaaga gacaagcttc catcggccgc agcgcacggc gcgatggcct tcgtgctccc
     3361 acgccagcag ttcgtgacct tcacgctggt ggccccgcaa cacgggcagc aggcttaagc
     3421 cgtcggcggg acggatgtcg cggccctgga agaatttcgg atattcagca cccgctgcgt
     3481 cgagaaccgt cggcaacagg tcgatcacgt gtccgacctc gtgggtgatt tctccgcctc
     3541 gggtgatgac cgacggccag taggcgatca gcggggtggc gatgccccct tcgtgcgcca
     3601 tgcgcttgaa gcggcggaag ggcgtgtcgc tgaggttggc ccagggcggg ccgtagccga
     3661 tcgacgagtc gatcgtcccg atgggcgcgc cgggcttgcc ctcatcaagg ttctcggcgc
     3721 agccgccgtt gtcggagagg aaaagaataa gggtgttctt ctcgatgccg cggacgcgaa
     3781 ccttctcgac gatgcggccg acgccatggt ccatgcggtc gatctgggcg gcgaagaccg
     3841 acatcttgag atcctcgcga tccttgtcgc agacgtcgtc ccatgcggcg gcgcccggat
     3901 cgcgcggcga gagcgaccag cgggaatcga tgatgcctaa ctcgatcatc cgatggtagc
     3961 ggcgctggcg gagctcgtcc cagcccgccc ggtaggtgcc gcgatatctg gcgatgtcct
     4021 ccggccgggc atgcagcggc gcgtgcgggg cggtgtaggc gacgtacaag aagaacggct
     4081 tgtcgcgccc ggcgtattga tcgatgtact cgacggcacg ctcggtgatg gcgtcggtca
     4141 tgtagaagct cggatcgacg ggctcgatcc ggcggtcgtc aagcatgagc atacggtgcg
     4201 gctcaggccg gaagtaattg caggccccgc tcaggatgcc aaagtagtgc tcgaaaccgc
     4261 ggaccagcgg acgatgctcg ggagccatac cgacgtgcca cttgcccgac atcagcgcgt
     4321 ggtaaccgct ttgtcggaga acctcggcga tggtgacgca gcgatcggaa agaccggtct
     4381 gatagccggg aagctggtag tcagcgcaca tgtggccgac tccgaccgcg tggggatact
     4441 gacccgtgag cagggcggca cgagacgggc agcagcgggc agcgttgtag aactgcgtga
     4501 atcgcatccc cccggcggcc aaggcgtcga gattgggcgt ggcgatttcc gagccgtagc
     4561 agccaagatc tgagaagccc agatcgtcgg ccaggatcag gatgatgttc ggccgttgcg
     4621 gcgccggggc ttggtcggcc gcggacggcg acacgcccgt aatgcacaat ggcagacaac
     4681 aagaaagcag gaaacgttgc agggtatcgc ccttcataga gcctattctc cagagttcag
     4741 gcagagatcg caacgccgtg tccaaaggag cccggcaacc acatggccaa gctccgctca
     4801 tcgcctgcgg aacggtggca aacggattac ggagacgggg tcttcaagac gtagatcctg
     4861 acgtagtcgt attcgcagcg ctgggtgtgg gccatctggc gcagaccgat ccaacccgag
     4921 tgcgcgtgaa tcggaccata cgtcttgcca tcgtcgtcat aagagagcga aagcacgtcg
     4981 tcgatcgtga gcctgatctg gccgccgcgt ttgtaaatgc gaaccacctg gaaggcatct
     5041 tgcggtgccg gagtgacaag atccttccca atggctgcca ggcggaagcc cctgttcttt
     5101 cgaaggttcg aggtgccacg accacccgac cagtacgaga tgtggtagtt gttgagatcg
     5161 ccgctgtggt attggctgaa agtcccgttc cgaggtttga tcggcggctc gaaaatgttt
     5221 tcgccgttgc acccgcgtgc gttgaagaaa acgatgttca gacctcggtt gcggttctcc
     5281 ggacgcacag cgaactccag cagaaaatcg gcgggcactt cgcgatcgag ccagcagacc
     5341 agatgattcg cggccgattc tgagcgggtc gattccccgt tgctctcgag gacaagcttt
     5401 ccattcgccg cagtcatgct ccggccgcct tcgaggcgcc aatccttcag ttctgagggg
     5461 tcttcaaacg acgtactgta aagaggatgt tcccagtcca cgtcgacggg gatgaacttc
     5521 tctccggtga acctcacccc ctcatccgtg tagggtgtgt gtcctgcggc ctcacaatgg
     5581 gctttaatcg actcctcgct gatcggttct tcatacagac cgacggcgct gacgatgagg
     5641 cccggccctc cgacggtcag cgtcacatcg cggccggccg ctcggaactg gcccgggtag
     5701 ttgaacggag acgcgttctc ctgccgcacg ccgtcgagga agaactcatt gcggctcgca
     5761 tcagcggtgt tccaggtcca agtcgcaagg taccatcgat cggccttcag gtgcgtcagc
     5821 agtgtctgaa agacgtaggg tcgactctcg ccggtctgcg gggtgccggt catcacggag
     5881 ccccagcggg tgtcaagcgt caccgacggg tcggtgcaga gaagctggac attgaccgcg
     5941 tcgctttcca gcaggctcag cgtcgggccg gctcctcgca gaagcttgtc gggcttgaac
     6001 cacatgacaa gggttccgcg atcatcgagc agcgaggccg ggagcgtggc cgaaaaggat
     6061 tgcgatgtgg ccggcgagcc gtcgggagcc ctggaatcgc ccggcgacgg aggagaaaac
     6121 agatgcggcc gcgggcttgg cgcaggtgct tgaccggagg gtcgagtctt cccttggaac
     6181 gtgcttggcg cggtcgcggg tgccgagttg ggcactactg cgccgacaga ggcccgccag
     6241 cgggcgagct gggcacggag ttgcgccgct ttctcaggca tcctgtgggc caggttttcc
     6301 ctctcgcgaa ggtcggtctt caggtcgtac agttcgacct ttccgttgtc gaagaactcg
     6361 atgagcttgt agttgccttg gcggatcgcc cccgcggaca ttccgccgag gaagtgcggc
     6421 cggtcgagcg ggtagtgcca atacaacgaa tctctctgca atgtccgatc gccgtcgagc
     6481 aacccgacga tgctttcgcc gtcgatcatg cggttcggtc gattcttcag gcccgccatc
     6541 tcgatgagcg tggggtacag atcaggcgtg attaccggca cgttgcaggt tttgcccgcg
     6601 cggcgcgagc cggggcgata gatgatcagc ggcacacgta tgccgccctc gtacaactgc
     6661 gacttgccgc cgcgaagagg agcatttgaa gtgacgcggc gttcgccgcc gttgtcggag
     6721 gcgaagatga gtaccgttcg gtcggcgatt ccgagctcat cgagcgcgga ggtgatgttg
     6781 ccgacgccct cgtcgatact cgccagcatg gccgccagga cggggttgtt ccgggtcttg
     6841 cccacaccgc gcttggccgc gaagtgctcg atcatttccg gtttggcggc tagcaccgta
     6901 tgcaccgagt aatgcgagag atacaggaag aacggcctgt ccttgtgccg tcggatgaaa
     6961 tcgactgcct cgcgattgag tcggtcggtc aggtattcac ctctcggacc cgtcacctcg
     7021 ggcatgaaca ggtagggagc aaagtagctc ccggcgccga tgtaacgggt ttccgagcat
     7081 atcacctcgt caaatccgtg cagcgagggt tcgccccgct tgagccggta gtccccggtg
     7141 aggtgccact tgccaagcag gcccgtggcg tagcctgcgt cacgaagggc ctcggccagc
     7201 gtgtgatact ccggcgaaag gaatcgctcg tcgtctgccc gcaagtagtc ggtgataccg
     7261 acgcgagccg ggtattggcc ggtcatcaac gccgcccggg ttggcgagca caccggtgcg
     7321 gccgcgtagg cgttggtgaa ctgcacgccg cgattggcca gccggtcaag atggggcgtt
     7381 tcgttgaaac ggttgccgta gcagcccagc tccgcccagc cgaggtcatc cacgaggatc
     7441 agcacaaagt tcgggcgagc cgttggcgtg acgaggtcgg ccctccgcga cggcgaggca
     7501 cacgaaagcg ccgaaaggcc cgtcgccacg cagacgacca ggagaccggc gcgccgcgaa
     7561 tcccgccctc gcgatgcggc gatgcgcttg caacagcgga cgagcggcat cttcagagac
     7621 aacgcgattg tctgcatcga tctactcctc cagccggtga accgccattt actgtcatca
     7681 tgatcaccgc cggggccgtt ttcggcgcgt cgggtgaacg cattggccgg cggaccccca
     7741 acaccgcccg acgcgcggcc gtttccccag gtcgccggca gccgtttcac gcaaccaccg
     7801 gtcagctccg cgaacgaagc atctgtctga gcttcgcagc catgtcggca taggccggat
     7861 cgttcgccag attgcggtac tccttggggt cgttctgatg atcgtacagc tcggtgccct
     7921 gccggccttc atcccattcg gtataacgcc agcgctccgt tcgcagactg cggcccatga
     7981 agccccgctg tcctgattgg gcggcggcag tggttccgag gcgggtccct cgcgaaacct
     8041 gggtgagcgc gggcttggac cagcttgcct gcgggtcgtt gagcaagggc accaggctcg
     8101 caccctcggc tcccttggga tcgattccgc agagggcggc cagggtcggg tagacgtcca
     8161 ccaactcgac cgtgcggggg catgctttgc ccttgccctt ggttccgggg gccgcaatga
     8221 tcagcggcac tcgcgcggat tcctcgaaca ggctcatttt ctgccactgc ccgtgctcgc
     8281 cgagcaacca gccgtggtcg cccaagagca agacaacggt gttgtcggca agctcaaagc
     8341 ggaccagcgc gtccatcaat ctgcccacct gggcatcgac gaaggacacg caggcgtgat
     8401 aggcctgaag gaagttcttg agctgaccct ccgtcaggcc gtagttcggc ggcctgacct
     8461 ggagggcggc ttcgggaatc aacgcaaggt gatccgctgg ctcctgcgga agcgacagtg
     8521 tcggaagcgg atgctgccgg aaataggctt tcggcgcgat gcagggaaca tgcggcctga
     8581 aaaaaccgca ggcgatgaag aacggccgat tcttgttctc ctccatcagc cgaatcacct
     8641 cgccggtgat cttgccgtcg gtctgctctt cgtccgtccc ttcggcggcg agaaaggaga
     8701 tggcggcccc cagcgagcct tttgggccgg tgtattggat cacttcgtct tcgtcatcct
     8761 tgtcgcgtcc acgggggttg attcgcagat tccacgaggg cgcatcgtcc agaccgtcgg
     8821 ttccgatctg accgggcacg ccgtagtgat agatctttcc cacgcgggcg gcaaagtatc
     8881 cgacctgctg aaacaactgc ggcagcgtga gcacgtccgg caggttctcg cgaaagtgcg
     8941 tgcgattctc cagtaccccc gtcgtgtccg gccgcctgcc ggtcatgaac gaagcccggc
     9001 tcggattgca tagtggatac tggcagtatg cccgatcaaa acgcacgccc tgtgcggcca
     9061 gccgatcgat gttcggggtg atcgcgaccg ggcagccata gcagcccagc gagttgttga
     9121 ggtcatccga gatgatcagc aacacgttcg ggcggcgggt ggctccggca tccgcgggac
     9181 gctgagcccc gacggctttg tccgccgccg cgtccgggat gccgttgccg gtacccgcca
     9241 gttgcgagca cgcagactga agaacaattc cgcaggtcca gagggtatag gcaacggtgg
     9301 tcaggtgatg gctcatcttc atctccttgt gtttgaagtg ccgtgtgggt tgagatccca
     9361 aggccgaccg cctttgtgcg ggccgcaact caagcgcact gtcatccgga aacaggcgcg
     9421 caatctgcac acgcgtctgg ccgtaaggtt ctcattcctc tcctccttga ggaccagtgt
     9481 agagggaaga taacaacggc ggcaacccat tgcgagagcg atcaacagcc gccgtgaccg
     9541 gcgtggatag cactgttggc cggctgcacg gattggaagg atcaggtgcg tcgtctcact
     9601 tcccctgggt cgacggccgg tccagggggt gatgtcgcca tttcggtggg gcttgaagcc
     9661 ggccggcggc tgcactgcgg gcggcatcgc tccaggaagc tcaaagcttc ataacgcgag
     9721 ccacatcggc gatcgggtcg gtagtgtaac ctttcccctg cggcggctcg gcatctttgt
     9781 cccagtccgg ctcgccttcg cgcatgagcg ccccataatc ggccgctccc ttgtacaggc
     9841 ggcgaaatac ggtgaagcct cacgagagca tgtgccggat cggcttgagc tgtccaaggt
     9901 gacatttcca aaagccccct gctccggtaa ccggcgatcg ccagaagctc aggcaaggtc
     9961 tggaggtcga gcagcaggcc gtatcggtgc ggccaacggc cacggtctcg catccgtccg
    10021 ataaaacgct tctttgcgtc ccggaccgtc ctgcaaacct ttccacaggg ttggcaagcg
    10081 gtctttcccg agagtatcag cagggaccaa gctcctacgg acctcctcaa ctcagtattc
    10141 aggcgcgctc tgatggaccc gatatcttct agggggcggg cgcgcggagc gaatcgggaa
    10201 agcgccttcc tccgaaatcc gacatgccgc gtttgtcagg ccgttggaac gaatcgtacg
    10261 cttgaaacag tggcgggcga cccaaggcgg gacatggata acgcatactc cagctcggaa
    10321 tccagccgga ccgcaacgac agcgcgcccg agatggcgaa cgctcgttcg cagcctgcga
    10381 ttcaaggcgt ccgttcttgt cattttgatt ctcgccgtcg tgacgagtat cagctcgacg
    10441 ctgaccaccc aggtgctggg gcaggtcttc tacgagaatg aatacgcccg gacccgtgag
    10501 tgggctcagt cactggccat cagtacggcc gaggcaattc aggcgcgaga cgcggagtct
    10561 ctcggcctta ccgttcagga tctgatcaag agcaactccg ttgcgtacgt ggtgttttcg
    10621 gatgccgccg gcgaggtgct ggtgtcggga gaagtgaaag ccggtcttct gcaatcagta
    10681 gtttccgctg acggccagac cctcagtctc aagaccatga acgtaccccg actggtgtgg
    10741 aacaaggagc tggggttggc ctgttccgag gtgagcgtgc ccgtgctttc gaaaagcacc
    10801 atccggccga cgggaacgtc cggtcgagcc attgtcggat atctgcacct ggcggtggac
    10861 gtgaccgcta ccaagaaaca acttgaactg gtgaaccggc gcgtccgtca gatcgcgatg
    10921 gtgatgctgc tgctgacggt gccatgcagc gttctcgtgg cccggcgggt ggtcgcccct
    10981 ctcaaagaac tctccgagac ggcctgcgcg atcgccagag ggtcgaccga tgcacgggca
    11041 cacatcaaca ccgaagatga gatcggtgac ctggctcagt cgttcaatac catggccgat
    11101 cggctgacga aatctcagat ggagttgctg gagctcaacg cggagctgga ggagcgagtc
    11161 gagcagcgaa cgcgtgagct gcaggaactg gccgcccggg acccgctcac cgggctgtac
    11221 aaccgtcgtc atttcagcga ggtgattgcc cgcgagtatg ccgcggcgga acgctacgac
    11281 gccgatctga cgtgtctgat ggttgacctc gaccgcttca aggaaaccaa cgaccggttt
    11341 ggccatcgga ccggcgacga ggtactggcg atcatggcgc acgtgatcag cacggagctt
    11401 cgtggctcgg acgtggcggc gcgtttcggg ggcgacgagt acatcattct tttgccgcag
    11461 acctccgccg cttcggcgac ggtgctggtg gaccgtatca ccaaccgctt caaggaggag
    11521 gttacccgca agtttcccga cgcgcccgtc ggtgtgagca tcggtgtcgc cagcctccga
    11581 accacgcgcg ccgcctcggc cgaagccctg attcacgagg cggacgtggc cctgtactcg
    11641 gtgaaggcat ctcgcaaatc cggtgcgctt cgcgacaaac ggccgcgacc tgcccgttcc
    11701 cgcacctgag gcctgcttcc tcgacacagc gtgggtgcag cacctcattc cgattccttg
    11761 taggtgctgc agatcagtgc gtccctgatc gcagccgcca gctcttcggc atgctgcgag
    11821 ccgatcagca gccgtcggcc gcctgccagc accaactgga cgcctcggtt gccgctgatg
    11881 ttgtaggcct ttccaccacg gccccagcgg atgccccacc cgccgaactc ccggataggt
    11941 cggtaggttc tcggccaaca ttccgtgatt tcgtcaaatc ggatgtgccg ccaccccaac
    12001 ggccaaaagc gaacgtaaag cccgtcgcct cggacctcgg tgatcagtct tgttgcatac
    12061 aagagccagg caaagatcag ggtgaccagt agcggtaccg caaatgccgc gatcagggcg
    12121 atgctgttcc aggtcaagtg ccccgcggga gacgccaata gcaattgtcg gaacaggaca
    12181 tagccgagaa tgatcgccat gcttgccggc catgccaaga ccatggccca cagccagact
    12241 tggcggaatt gctggacctc tgtgaagata acctcaggtg aacggctgcc gggcatgata
    12301 tcgaactccg tgagctttcg tcggctcgta ccgcgcttcc acatgccgcg gattgtacct
    12361 cttgtctgat catgacgcga tcacgatagc cggatcgaag tgcagaggga tcggccaagt
    12421 tccgggatgt ccttcggaac cgtgaagctc cccgcggttg agaaactgtc ccgcaacccg
    12481 tgtggaaccg gcttccggcc cgtcggcttt cggggtttgc aaataggctc tggacaacgg
    12541 accaagggca cgccgggccg caaagggcac ttagcgcgtg aggggcgctc cctttcggct
    12601 ctgccagtgg gccgcgccgg agaggtttgg ttgactcggc agaccggggt cgcggttcgg
    12661 aaagaagtcc gtaatgatac cggggcgcag gagctgtcgc agaacagtct cagccgccct
    12721 tttttcgcag tatgtggaga tacccgcgcc gatagtgtaa aatgggtttc tgcccgaagg
    12781 ggcgaaaatg cggaggaacc atgaccaggc aacaaactga aacggaacgg gctctttgtt
    12841 cacggcgtct gttgctggct tccgcgatac tcctgagcat tccaacgggc cgattggtgg
    12901 cggccgattc ggcctatctg tacggcatcc actggtgggg ctatacgccg ggacaagcgg
    12961 tcgacacggg acctgcggca atgctcgact gtccggcgtt cggcggatgg gacgttgaga
    13021 cgatcgtcac ccattcggcg aactggtggc aggcttcctt cttcacgggg ttgtaccagg
    13081 atctctacac gaacaaaaac gtcaccgtta tcactcgcat cgactacaac tggggcgaga
    13141 cggtcccttc gccgaccaac cccgattacg cgagttggcc gaccagcgtg gtcaacaccg
    13201 tcaacacgct ccgcaactac tgccacatct ggctgatcgg caacgagccc aacatcgtca
    13261 gcgagggcaa tagctggccc gacaaccacg tcacgcctgc cggctacgcc gcgatctacc
    13321 gcaacgtgcg aaatgccatt cacagctcgg ctcaagccag cccggccggg gagcacgttg
    13381 tgctgatcgc cgcccccagc ccgggcggcg tcatcgccgg tgtccgttgg atggacggca
    13441 accagtggct cgggcaagtc attgataaca tcccggccgc cgagattgac ggtatcgctc
    13501 tccatgccta cggaggcagc ctgacggact tccacaacag ttacgtctca caactgaacc
    13561 tgatcgacgg caaggggctt caggatcggc cggtctacat caccgagtgg aacaaggttt
    13621 cgaccgagcc ggtcatcgct cagttcgttc gcgactgtta tgcggatctc aatacctgga
    13681 accagacgcc cggcaagcac aacatccgct gcctgtgctg gttcatatat gattgggggc
    13741 agcagaatac gaatccggat tttcagaata tgtcgatcga gtactggaag aataacggcg
    13801 agccggcggg cagccccaac gacctgtaca ccgcgttcca gcaaaccgtc gaccttcgct
    13861 accctgccgg tctggtcggc actcggccga tgggtcgttc tatcggccga tcccccgaaa
    13921 gctttgctcg cgaggtttac tatcgcgacg aattggcgaa cgacaccttt gaagtgtgga
    13981 atgccggaac cgacacgctc aactacgcca tcaccgacga cgcgacgtgg ctcaacgtca
    14041 acccgaccgc cgggtcgtcg accggcgagc acgatacgat cacgatcctc tatgacacag
    14101 agcacctcgc aatcggatct tacctggcga cgatcgcgat cagcgacccg gcggcctcgc
    14161 cgccgactgc catcgtgacc gtctcgctca gcgttgtcga ctccccgtat gctccagccg
    14221 acttcaaccg cgacaggttc gtcgatgaga ctgatttgga cctctttttg gattgcttga
    14281 ccggtccgaa tgccggtccg ccggccaccg ggtgcaaacg caccgacatc gattgggacg
    14341 gcgacgttga ccaggatgat ttcggccttc tccagcggtg catcagtggc actggcagcc
    14401 cggcagatcc cgactgtgcc gactgacagt acggcctaat tgttgtcgtt ggtcgtcctc
    14461 gaggtcgtcg ggacaacgga cgaatttcct cgacgagcga gggccgcctg gagcttggta
    14521 cgaacatccg gtgccgcttc atcaagcagc ttggcgatgg cggctgtgga cgcttggccc
    14581 cgcatgtgat ggagggcgtt ctcggcagcg tcacgggtgg gtcccgccgg gccggaaagc
    14641 agcctcacca gctcccgtac gcctgcggaa atcccggcgt ctcgaatggc cctgatggcg
    14701 gccagtcgca cggactcgtc agcatcgccg atagcgtcga tgattgttcc ggcggcggtg
    14761 tcattcatcc agcggccgcg acggccgaga atctcgatca ggtcggcttt cagcggccct
    14821 gcggcctgag tcatctgctc ggccacggcc gtggtcaggt cgttgcccgg aagcaactcg
    14881 cacgcctgaa cggcggccgc acgaacgcgc acgttgtcat ccttcaggcc gccaaaaacg
    14941 atgccgaccg cttccttcgt ggccagtcga ccacacgcat gcaccagacg acaacgctcc
    15001 ggaacgctca gcgatgacgc gtcgggcagg atcttgaaga cgtattccgc aggctggaac
    15061 atctcggcgt ccatcagcgt ctccatgaga cgaatcaggg ccgacgcgga accaggtctg
    15121 ccgctcttga aggcttgggc tagtacatcg aacgactcgg cagatggcag cattgccaga
    15181 gcgtccatca cggcgcgggc ggtcgccgga tcgccctcga tagcgccgcc ggcgatatac
    15241 gaggcggccg cagggtcgcc ccgcaaggca agcgaggcaa tgacgcccaa cctggtttca
    15301 ccggtcgtga tctggacggc acccatgagg cactggcctg ccctgcgatt cgggattcgg
    15361 gccagcgatt gtcgggcggt ctcggccagt tccggagtgt ttagcgcaat gtacagcgca
    15421 tcgacggagg cgttttccgc cgcgatgaaa cccagcaacg cacagatatg ccgcctggtc
    15481 tcaatcgggt ggttggcctt cagttcacgc accagggctc gttcaacatc gccgccgtta
    15541 ttggtgcccg cgcagacctg caccatgcga aacagggtct cataagccat tttaccggcg
    15601 gtcgggttct cggcggttcg tccgagcaat cgtatcagcg ttggcacggc cggcgccccg
    15661 accgcaacca gatcatgtaa cgcctttgtc tggacgtcgg gatcgttcgt tcctagagcg
    15721 atgatgcgcc cgtcgtcggc cggaagactc tgtcccatgg ccggggagac ggccaggaac
    15781 actccgacga gccctgcagc aatcgagatt ctcatgtaca cgttgctcct gcgatggagt
    15841 gggcgtgcga tgcgctcctc gttacgtcac gcgaaccagg gggcacgata gggcttgtca
    15901 agccagcggt ccagggcggg atcattgatg attatctctt tctccggatc ccattgaatc
    15961 gttcgcccgg tgcgaatggc gatgttgccc aggtgacaga cggtcaccga tcgacaaccg
    16021 atttcgacgt cgcagatggg acgcttgcgc gtacggacgc agttcaggaa gtcacgctcg
    16081 tggccgaggg catcttcgcc cggcgaacgg tagagatgca cgtctccggg gccaagaggg
    16141 tcgttgagaa tcgccggatc gccggcttca atccaccctc tgccgacctc gatccagccg
    16201 tcggtgcctt ggaagcggac accgttgcgc ccgtggctta cggtttcgag cacaacgccg
    16261 ttggcataag tgtgccggac atgaaagctg actgcggtct caaacagccc gcgggtcggg
    16321 aaggccgcgg agaccggctc ggtcttgacc gggccgctgt tgtccatgcc caggccccac
    16381 tgggcgatgt cgttgtggtg ggctccccag tcagtcatct tgccgccgga gtagtcgtag
    16441 aaccaccgga aatccgagat gcagcgcttt ggcgtgtatt ccgcccacgg ggccggaccg
    16501 agccagaagt tccaatccag gcccggcggc gggggcacat tgggttccca atcgcagacc
    16561 ggcccgccgc cgatcgtggc ccgcactaac cccaccttgc cgatacggcc gctgcgaacc
    16621 agctcgcagg ccagccggaa gttgctgcac gaccgctgct ggctgccgac ctggaagacg
    16681 gtggcgttac ggcgggcggc gtcaaccatg cggcgggcct cgcggatggt gtatgagagc
    16741 ggtttttcgc agtagacgtc tttgcccgcc tcgcaagcgt ggatggcagt gagggcgtgc
    16801 cagtgatcgg gcgtggcaat cagcacggca tcaacgtcct tgcgagccag caactcgcga
    16861 tagtcgttgt acgcgggaat gtccttgccg taggcctccc gaaccatctt catggcctcg
    16921 ttgcggctct ggtcaaagac gtccgcgatg gccaccactt ggacgtctgg aaactgaagg
    16981 aagttgccaa ggtgcccccg tcccatgccg ccgacgccga tgaagcccat gccgatgcgg
    17041 tcgttggccg gtcttgtttc ggcgccgccg cgagcccggc cggtgagcac gtatgggctc
    17101 atcgccgtgg cggtcaagat gcgtgctcct tgtccaagca gtcttcgtct cgatatgggc
    17161 agatgttcgc tcatgccggt tcctctgcgg cttcgccgaa ttgcctcgtc gaacgggccc
    17221 ttgcggccga cgtccggccg cgcccatacg caaggttata ccataacctg gcgagtgaaa
    17281 gcacgtccga cggcttccca aatgggtgga catcgcttag tctgcgccat cgcgaagctt
    17341 gttgtttgag cggacgcgtg tgagtgtgct gacagtgaga cttcttggcc atgaacagag
    17401 cgattttgaa gacgtcgtgt cttggtgtgc tgcttggcag tcccgtaacc gttcagaccc
    17461 catcggtccg atctgccggt agcatacccc ccttcaa
//
LOCUS       DAOTPW010000116        17190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3310_length_17190_cov_5.764167, whole genome shotgun sequence.
ACCESSION   DAOTPW010000116 DAOTPW010000000
VERSION     DAOTPW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17190
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_17190_cov_5.764167"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..821
                     /gene="nuoB"
                     /locus_tag="P5316_11820"
     CDS             63..821
                     /gene="nuoB"
                     /locus_tag="P5316_11820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRR85686.1"
                     /translation="MLSILTARLQQKHRTLAYPAAEPVLPDRLRGRPTIDGSKCGDGC
                     DACARVCPTQAIKAEGKSTSIDLGRCLFCAECAQACPAAAVAFTTEYRLATRRRDDLV
                     VGSDSYRLAEALDAQSRRLFGRSLKLRQVSAGGCNACEADLNVLTTVVFDLSRFGIQF
                     VASPRHADGLVVTGPVTANMKQALLKTHEATPAPKLVIAVGACAISGGVYSNHEEVLG
                     GAAACLPVDLFVPGCPPHPMTILDGMLRLLGRIS"
     gene            complement(844..2364)
                     /locus_tag="P5316_11825"
     CDS             complement(844..2364)
                     /locus_tag="P5316_11825"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR85687.1"
                     /translation="MTLAELVLKIAGQSDPKVRREAAVQILRLNSPEGVQALLPILAS
                     SNNEAAKLAVCEAIAEVRSKDSLFRQPLLSLLDHKEPTLREAAVAALAGYRGDVVVDQ
                     RLAEIDRQLVIDEWVTLAREFYTLLPTDADRTERLKLWLASRRSFVRETALEAIYQAL
                     KKRQVEPAPVILAQLRTMIDDPEESVRRKVIEILRDLRQPEDSHLLEERLAKEESPAT
                     KELIYHALGYLQNPASIPVCLKGLQDPADSVAAKAAGALASLIEFAKSSTGTVDIQPV
                     TRALLQRAEAGLGDDKLRAAVIDAMASIAAPEFLDVLVKYAGSDEYVPAIRQSAIRGI
                     GAAGKPAEDRLPLVIDRLAVEQDPGVREAAVTALGHLGSRPEHLVPLRTRLDPKVELS
                     VAVQKKAWEAYQAVFMRMIPKDRMQVISTYPDEAIRLVAEVQLAPESRQVIAEQVLDY
                     ARDKAKTDPLGVLAFLDRFSQAVPPDRLGPAWAASLADLRKQIQSTTQPAPGAAAG"
     gene            2877..4589
                     /locus_tag="P5316_11830"
     CDS             2877..4589
                     /locus_tag="P5316_11830"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HRR85688.1"
                     /translation="MRRCCVLAASFLILARVSVVAGAEPGVSVDGNKLTVRTATVEAV
                     FNGMAVVSLTSADGKVQFVGPEPPAHGVDLFYLNGNNLGQDKHQRVSVREISALAARI
                     VVEGDDSHRSLLVAVDPDTHDVRVTPDGLSSRRGLRSVGWRVPFAAESTVILPVVNGM
                     QLKPHQPWPGNGRFLWPYQWCAQLAVVERDGESLMVHCEDTQMRFKAVRVTRNGPKTE
                     LCFESEPTGPLWDNRTAGGIEWRINAYKGDWKVPATRYRDWLYKTYDLAEKRKHRPAW
                     VDKITLAVCWADAGSEAFLDALAEVHPPEETLIHLSGNWRNDKYDVNYPDYTPNDKGL
                     GYMAKARKMGFHVMPHFNYFAIWMKHPFFQEVRDFQIRSVDLNSPEGWYWPPQTYDYT
                     RMAYIHPGLGLWRDKLVDVLLETCGKMETDAAFIDQTLCTWNTDNGQVQGMNTIEGMW
                     QMQEEFAAVRPGLVLAGEGLNEISFQRQCFAQAHIPDGWGDLEAKHPQGVHPIGSFLW
                     AGHTRLIGYHHLNPDNKDFDLGVEVYERMDALPTIVSGNPKHLKEMSAGTKKIFERAK
                     KKGS"
     gene            4881..6869
                     /locus_tag="P5316_11835"
     CDS             4881..6869
                     /locus_tag="P5316_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85689.1"
                     /translation="MKRLILLTAAAGLFCLAVFLLIHWGPADVRVHADEPLPPPVPGE
                     PLALAVAAENNADYAQAESIYRAIISSYPGTAEAWTSQARLVSIKLALKEWDQAVTEC
                     DILIAQGPDHPDLAATVLETARVLSKSGKTKKAIRLFEYHVGRWPDSSATLEIKKELA
                     LLYARSGNPAVDSLFQELQNTLPPSDDLAELTAGIGAAIFARAREQQSREAYATALGY
                     LQTAEQMYQTNTPSWNTERRAIQARQRIIQAYRAMNDRLAAGLAEDQLLAAYACRDSE
                     HALADALCVLADEHRASDSHARACELYQHALSYCPGSARTCDLMTGLARSSIALADYD
                     TAQRMVNQLATSYSAQADFIRRMNEIAWDYHVAKQNELAEGIYRYVVDNFGDRKEAVF
                     SQSALVIVKYVTGQDEVAAAELTRLFDGFPDNKRKPAALKQVAQACEGLKKYRQAAQA
                     YRLMLQLTSDPLEAERVTLDYIGATMRAIQTQEDAEALLQYCLEVLKTRPTNPTLVHD
                     AVGSPVRGFGRIAILTGRKSLSIEAADRLMAALDPDAPEYQHALARHVRAWAFSWNRQ
                     AEEAIRQMVNLADDYAGTTDKNVERLCATSLGHAYGYALANLGRPDLAQQYLDQIRSR
                     WPDGRHESVIELYTTHVPASVKKLAEQGAAIMQGKPEQ"
     gene            6957..12347
                     /locus_tag="P5316_11840"
     CDS             6957..12347
                     /locus_tag="P5316_11840"
                     /inference="COORDINATES: protein motif:HMM:NF015055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-arginine deiminase family protein"
                     /protein_id="HRR85690.1"
                     /translation="MYTHASTVGSLGRDAGSRRAHVLPYVLIALLACWQSPDQAAACG
                     PCFGYFCGECADCDPVEERCIPNHHSCCPCSYWGTYRYVDICVWPDLTWLGDVVRVHV
                     VGSSAAPAVCYQLELFYDSRFVENDIACPLEYPQEIIQPTLPSYQSCAGYSMPLPSDP
                     SKGWLALPGSHDFYVQPMRPRFQLARLEASPYNPYTTASRYFAVADLDLRGPYTLDPT
                     CHPGSPCGCSSGNDLERCEHPDDYPCQPGDPAIFYIGVNDDFDEGKETEDAEDVSILD
                     NTSQPALNDLAQMCWTMGTATVYEAYWSGTYDVMCGDSFEERVSAKMTVSPGEIVRLW
                     VWHNSQYEQLLLGQNIIPGYGTDSSFYAEGLKPGTVTIQLEGKIDNDDSRRRIDKIRL
                     SAVKIDVDVDSDNTNTTAPFGPDAPPATPPAANVEDRIEQKAGEPGRFVLVNDDDDDQ
                     DGILDYDDGYNKDENPIFLGDDVTYEEDDFVMLKVKIAPTSINLDPNQAWVLIDYSAD
                     SPDPNVWSIGRIRLWKKPGDEPRDRRSALTTGQGDWIEPGLYTDLKTLMPWDPNLQPD
                     PGNPPASHEMTFYIEGVNASGSLGDTDITFRIDPDGPGPAGCVHADTAQVTVVRPNLG
                     ADSNNDGFYNGADEIIEDRSDLSGLYVGMNDNFDEEDDLEDFETPQASKKPCPAGQYA
                     VVPTNDLEPVYVSLVPGGDSFYGECDLYLKGGRDHLRVYAIRWGSCDAACPATGENVC
                     WKLIDENYDLAANYLHSQGPIYGWALYVEGILPGEAQMLLEFWHDGHKLYEDWVNFTV
                     VQIDVDVDSDNTNTNAPYGPDAADPMTEDRIEEKNGEPGRYVLVNDDDDNKNGKLDNS
                     VLEVGPIQDEDDLVEIKFTAPDFTPHNPDYVKWLLNVPANIDIWQNKNKSGFHVWGGD
                     VAEIPWTDHALPQTWYMQGLEVPSSGPVEIEASLWIDPDEDGPKSYRLVHRDTVQVTV
                     IRVGLDVDTDRDGTVNHLLDESRKDEITRDRGALFMTNYDDDDANGHPDAVSFNNEGQ
                     PIDENTDIEPGDVPDIGHLVVRAPGFATTGLQFFLRMDADDLKAVHIFEERAEGKHVL
                     KTSSGADWPGWTAQSSASGDASLDITALVNPTLDVDLGIEALFLRGHKTRSPSYEFDG
                     RVKVELVVQLTTGATTINPAKILKVDTVQLAVAPYLLLPNTQACQEVWLHTWTEANPI
                     ATALGALAHRVSSGGGQWLQDHVQVGMTSIPGLQMHVTMRMPYYRGQPGWPASSLLGP
                     QRGVYRFRNTCTHDGADSGDFGGNLEIIPYSDDWPLGRIIVGNTMSTRLRSFLQAQRI
                     AGSTAIQEPIVADTSWLAVGHIDEIMCAVPGGARGYKIVMASPDYAHQLLTTGDSARG
                     IAAPSEDAAVFATGAQASGTASNAITTTSMVWLFDGAAHGRVQCVASGDISDGETVTL
                     SDGTTTKVFEFDKAGDGVDTGHVQVDIVSAGTAADVRDALQSTIAAAGMGVTPYADGS
                     DALIVINNALGTAGNVPITDTVTASAFSVQGMAGGEASASVRDFRGSGWEWVRLFSGT
                     GAGQVGHVSGQGIGWISVAQVFNTTSQIASATGIYGLTFGSAHLAHQPSGWFTEPGSG
                     TRYLIVTNSKNWTGYDGTSEIDFPAAMAVYEVLHDTDLWSLNTAAQGRIEGVRAQIQT
                     SMGSGYLLHDTTTADDSLSGDSDDDFIALPTIFFGPSGTLAARHNAAFTPGLANGLAA
                     TGSLIVYPQQFGARSGAVDVFEAATTAIMGSGMTHFADDWDGYHRLEGEVHCASGEVR
                     TLFSFNWWEHQP"
     gene            12344..13444
                     /locus_tag="P5316_11845"
     CDS             12344..13444
                     /locus_tag="P5316_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85691.1"
                     /translation="MKSIPRFGNQSFWMAVALLAGGLRIAWANSSAEEVVHQAVAICC
                     QAPTGKAVADRPNAWPDFDKLIAGLSKSMLPDLRAIASSETHTKLERRVAQVLCRRIE
                     HPEEFQKVEPFLTPVENPTGRIYGPLASGKRRPSIALKPIDDPELLAPQVRAAIERNG
                     KLYEDLRTEALRIRQRINSLPDGDMPSLNQIYADLDTRIAELKRQYHVEPGQTLELVF
                     ACGDPRQRPPLDPKYLPAWEEAVTRASSPEMKANLLQVVRSFRSRDSVPVVAACLSTA
                     SKSGDEGILWASLAFLLEVEPTETAVDEISTALDELQGWMRSSAEQQVRSAVSRSPRW
                     QTFLQQMKADPKWSAKADRLLRQTASTTAPGD"
     gene            13600..14946
                     /locus_tag="P5316_11850"
     CDS             13600..14946
                     /locus_tag="P5316_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85692.1"
                     /translation="MIRADLRAGEPGRFVLVNDDDDDQDGILDYDDGYNKDENPIFLG
                     DDVTYEEDDFVMLKVKIAPTSINLDPNQAWVLIDYSADSPDPNVWSIGRIRLWKKPGD
                     EPRDRRSALTTGQGDWIEPGLYTDLKTLMPWDPNLQPDPGNPPASHEMTFYIEGVQAS
                     QALGDAKITFMLDPDGDGPAGYVHLDTVQVTVIALTASPGSGPVGTRIDFEASPTGLG
                     IFDGSEAISFRGTFEPTGYCKTCEFTSEHQAHFDSAYPDRVSIILGEDTSSDICFLFN
                     LGVIFRAPGVLRGDIAIETASIKCRIHGLAFSVTPSAAIGVLDGSTFLETSETDILHA
                     ETGEDVTNAPATYLHDARVLVPVNGINVKSDQLPDATTEVAIKSFSVFGFEQGSEALT
                     LRLEPPGTLQSTLYTYLNTGAGRRRIIFVDNRSLEGVYSDRIAVYAPFPGKVRVVK"
     gene            14958..16271
                     /locus_tag="P5316_11855"
     CDS             14958..16271
                     /locus_tag="P5316_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85693.1"
                     /translation="MRCIPVFRLAVRVVRFGVAPSGVLLLLAAALSTLSVGCDGGTET
                     KRANGPPPRVHLMKPYTDGWTDSDISPFGDVVLKDQKLRIRVKLDQAVQQKSDITVEI
                     TFDNQSWQPLSLKDADALVDAGKELRVSVPWVDMLSQGWTADGEDAVPEQASADSSAD
                     PPFDETPPESGDYPNNPSSNRADSDAFDGCGLASAQRGKARGPGNETLWPPESVINKQ
                     FLASAGVVYCKVRATALNATGFANVPGQLQNQADWFYYSGHGFNRRYTGDGVGRLLAA
                     DAQNGLAAADAKWSPDVKHVIIAGCSMININDPNRDCSACEDDGILWAQTGPTHIAGY
                     NWKAPSDNVYPGPNNYTAQIVGHFCTRLGGESFATAWLNANADMDPDKLHVRASPDEQ
                     GGPGRVWNAAAIDLSGGPGNGVYYYWDIATNPARPEIKMIPQSQW"
     gene            16427..>17190
                     /locus_tag="P5316_11860"
     CDS             16427..>17190
                     /locus_tag="P5316_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85694.1"
                     /translation="MSGKLTLVVAQRVEASGEANDRLQKAVDFYQAYVKTSAEMNERA
                     GPNRELTPSEMQRVLHSRLVAIEQFVEVNKVFPETSAGLTAILWVGSLVHGQSSELAA
                     SVLPALRELRHRHGASWQGALASHVESPLLYVSLPPQGTPDARRRALYEAQYQLMLET
                     LPKGEVSVDRTLPGVDLLLRDRLAVKGSIRASFLQWIRACEYNLSYLSRGEEAREWLR
                     KSIATNQRIMTEYPGTEESKDADGRTYQFARRAAEFA"
BASE COUNT         3528 a         5110 c         5321 g         3231 t
ORIGIN      
        1 cctggcgcta tcgccaaggc cattcttgcc tcgaaagtgt tcaacttggg ctaggagttg
       61 acatgctgag catcctgacc gcgcgtctgc agcagaaaca ccgcacgctt gcctacccgg
      121 cggccgagcc ggttctcccg gatcgtcttc gcggccgacc cacgattgac ggctccaagt
      181 gcggcgacgg ctgcgatgcc tgtgcgcgcg tctgcccgac gcaagcgatt aaggccgagg
      241 gcaagagcac cagcatcgat ctgggccgat gcctcttctg cgctgagtgc gctcaggcgt
      301 gtccggcggc agcggtcgct ttcacaacgg aataccgact tgcaacacgt cgtcgcgacg
      361 acctggttgt cggctccgat tcctaccgtt tggctgaagc gctggacgcg caatcgcgac
      421 gcctgttcgg ccgctccctc aagcttcgcc aagtcagcgc cggcggctgc aatgcctgcg
      481 aggcggacct caatgtcttg accactgtgg tattcgatct cagccgattc ggcatccagt
      541 tcgtggcctc accccgccat gccgatgggc tggtggtgac cggtcctgtg accgcaaaca
      601 tgaagcaggc tctgctcaag acccacgagg ccacgccggc tcccaagctg gtcatcgcgg
      661 tcggagcatg cgccatttcg ggcggtgtgt acagcaacca tgaggaagtg ctcggcggtg
      721 cggccgcctg cttgcctgtt gacttgttcg tgccgggctg cccgccccat ccgatgacca
      781 ttctcgatgg catgctcaga ttgctgggca ggatctcgta gcgaaagatc ccgcaaccaa
      841 ggttcagccg gcggccgccc ctggggccgg ctgagtggtc gactgaatct gcttgcggag
      901 atcggccaag cttgcggccc aggcgggacc gagacgatcg ggtggaaccg cctgagagaa
      961 tctatcaagg aaggccaaga cacccagggg atcggtctta gccttgtcgc gggcataatc
     1021 gagaacctgc tcggcgatga cctgacggga ttcgggggca agctgaacct cggccaccag
     1081 ccgaattgct tcgtccgggt aggtggagat gacctgcatg cgatccttag ggatcatcct
     1141 catgaagacc gcctgatacg cttcccaggc tttcttctga actgcgacgg aaagctcgac
     1201 cttgggatca agccgcgttc ttaagggaac gagatgttcc ggtcggctac ccaagtggcc
     1261 gagggcggtc actgcggcct cgcggacacc ggggtcttgt tcgacggcca gtcggtcgat
     1321 gacgagaggc aatcggtcct cggcgggttt gccggcggcg ccgattccgc ggatggctga
     1381 ctggcggata gccgggacat actcgtctga gcccgcgtat ttgacgagga catcgagaaa
     1441 ctcgggggcg gcaatactgg ccatggcgtc gatcaccgcg gcccgaagct tatcgtctcc
     1501 gaggcccgcc tcggctcgtt ggagcagggc ccgggtgaca ggttggatat cgacagtacc
     1561 cgtcgaggat ttggcaaatt cgatcagaga tgccagggct ccggcggcct tggccgcgac
     1621 ggaatccgca ggatcctgaa gtcccttgag acaaacgggg atcgacgcgg gattctgcag
     1681 gtagcccagc gcgtggtaga tcagttcctt ggtggcgggt gattcctcct tggccagccg
     1741 ttcttcgagc agatgggagt cttccggctg ccgcaggtcg cgaaggattt cgattacctt
     1801 cctgcggacc gactcttcgg ggtcgtcgat catggtccgc aattgggcaa ggatcacggg
     1861 ggcaggctcg acctgtctct tcttgagggc ctgatagatg gcctccagcg cggtttcacg
     1921 cacgaatgaa cgtcgtgagg ccagccagag cttcagtctc tcggtgcggt cggcgtcggt
     1981 gggaagcaac gtatagaact cgcgagccag ggtcacccat tcgtcgatga cgagttgccg
     2041 gtcgatctcc gcgagtcgct gatcgacaac gacgtcgccc ctgtagccgg ccagcgccgc
     2101 cactgccgct tcacgaagcg tgggctcctt atgatccagc agggatagca ggggctgccg
     2161 gaaaagagaa tccttgctgc ggacttccgc gatggcctcg cagaccgcca gtttggcggc
     2221 ttcgttgttg ctgcttgcga gtatgggcag tagagcctgc acgccttcag gcgagttgag
     2281 tcttagaatc tggacggcgg cctcgcggcg taccttagga tcgctttgcc cggcgatctt
     2341 gaggaccagc tcggccagcg tcacggcatc ggaagcagca ggactggaag acggggccga
     2401 ctgggtggcg agtgcggccg agactgtcgg acgcgtgacc aataatgcgc agactaaaac
     2461 tgaccaacag aggcgcccag ccacagatcc cggccagagt tgtcgcaacg ctttcctgcc
     2521 tcgcagacgc cttttcaccg tttcttgttc cggagcccgt cgtgccaccg tcctgaagtc
     2581 tcgcttctac cctgtcgcgg aagaaatcgc aagtcatccg tcttcagaga gcgcaagtcc
     2641 acccgttggc ccggcgctgt tcgaggtgta cggcggtccg cagccagaca tcgaggctat
     2701 tttcacgagc atcggaaatc cgtcatgggt gcaatcactg gaatgagtgg tctggcattg
     2761 gtctttcggg ccgctctgcc ggctggtgct gccgggtgac atggtcaata ataggtccgc
     2821 tcaagccata accctgtttg gaaccggcgg gttctccaag cgaaacggag gtttcgatgc
     2881 gacgatgttg cgttttggca gcctctttcc tgattctggc gcgggtctcg gttgttgcgg
     2941 gagccgagcc gggggtctca gttgacggaa acaagctgac ggtgcggacg gcgacagtgg
     3001 aggcggtgtt caatggcatg gccgttgtca gcctgaccag cgcggacggc aaagtgcagt
     3061 ttgttggacc agagcctccg gcgcacgggg tggacctgtt ttacctgaac ggcaacaacc
     3121 tggggcagga caagcatcag cgggtgagcg ttcgggagat ttctgctctg gccgcccgaa
     3181 tcgtggtcga gggggatgat tcgcaccgct cgctcttggt ggcggtggac ccggatacgc
     3241 acgatgtgcg cgtcaccccg gacggattgt ccagccggcg agggcttcgt tcggttggct
     3301 ggcgggtgcc gtttgcggct gagagcacgg ttatcctgcc ggttgtcaac gggatgcaac
     3361 tcaagccgca ccagccttgg ccgggaaatg ggcggtttct gtggccctac cagtggtgtg
     3421 cccaactggc ggtggtcgag cgggatggcg agagcctgat ggtgcactgt gaagacactc
     3481 agatgcggtt caaggccgtt cgggtcacgc ggaacgggcc gaagaccgag ttgtgctttg
     3541 agagcgagcc gacggggccg ttatgggata accggacggc cgggggcatc gagtggcgga
     3601 tcaacgcata caaaggcgac tggaaagtgc ccgccacgcg ataccgcgac tggttgtaca
     3661 agacgtacga cctggccgag aaacgcaagc accgaccggc gtgggtggac aagatcacgc
     3721 tggccgtctg ctgggcggac gccggcagcg aggcattcct tgacgccctg gccgaggttc
     3781 atcctcctga ggagaccctt atccaccttt cgggcaactg gcgaaacgac aagtacgacg
     3841 tcaattaccc cgactacacg cccaacgaca agggcctggg ttacatggcc aaggctcgta
     3901 agatgggctt tcatgtgatg ccgcatttca actacttcgc catctggatg aagcacccgt
     3961 tctttcagga ggttcgcgac ttccagattc ggtcggtgga cctcaacagc cccgagggct
     4021 ggtactggcc gccgcagacg tatgattaca cacggatggc ctacatccac cccgggctgg
     4081 ggctgtggcg ggacaagctc gtcgatgttc tgctggaaac ctgcgggaaa atggagaccg
     4141 acgcggcgtt catcgaccag acgctgtgta cctggaacac cgacaacggc caggttcagg
     4201 ggatgaacac catcgaaggg atgtggcaga tgcaggagga gttcgcggcg gtacggccgg
     4261 ggctggtgct ggccggagag gggctcaatg agatttcgtt tcagaggcag tgctttgccc
     4321 aggcgcatat tcccgacggc tggggcgatc tggaagccaa acacccccag ggcgtgcatc
     4381 cgatcggctc gttcctgtgg gcgggccaca cccggctgat cggctatcac catctgaacc
     4441 cggacaacaa ggatttcgat ctcggggttg aggtctacga gcgaatggac gccctgccca
     4501 ccatcgtgag cggaaatccc aagcacctca aggagatgtc tgcgggaacc aagaagatct
     4561 ttgaaagggc aaagaagaaa ggttcttgac agtcataggc gctcgaccga ggttgtccag
     4621 gcggtttcag ccggattgca tcaaaatttc cgaaaaagtc cattgacaga tgaacagcct
     4681 tggtgtatta tttgggcgaa gtctcgagta gctggaagga cgagtagatc gtaggcgcag
     4741 gaacctactc ctactcctac tcctactcct actcctactc ctactcctac tcctactctt
     4801 actcctactc ctgcctacaa tcaatctgcg atccgcagcc tcctttcctt catggcagcg
     4861 gatgcatggg aggtctgcgc atgaaacgcc ttatccttct gacagcagcg gctggtttgt
     4921 tttgtctggc ggttttcctg ttgatccatt gggggcccgc cgatgttcgc gtacatgccg
     4981 acgagcccct gccgccgcct gtgccgggtg aaccgttggc tctggctgtg gcggctgaga
     5041 acaacgcgga ctacgcccaa gctgagagca tctaccgtgc aatcatctcc tcctatcctg
     5101 ggacggctga ggcttggacc tctcaggcca gactggtgtc gatcaaactg gccctcaagg
     5161 agtgggacca agcggtgacc gagtgcgata tcctcatcgc ccaaggcccg gaccatccgg
     5221 acttggccgc gacggtactt gagaccgccc gcgttctgtc caagtccggg aaaaccaaga
     5281 aggccatccg gctttttgag taccacgtcg gccgctggcc cgattcatcg gccaccttgg
     5341 agatcaagaa ggaattggca ctgctctacg cccgcagcgg caaccctgcg gtcgacagtc
     5401 tctttcagga actgcagaac acccttcccc cctcggacga tctggctgag ctgaccgccg
     5461 ggatcggagc agccatcttc gcccgggctc gagagcaaca gagccgcgag gcatacgcca
     5521 cagccttggg ctatctgcaa acggccgagc agatgtacca gacgaatact ccctcatgga
     5581 acactgaacg ccgggccatc caggcccgcc agcgcatcat tcaggcctac cgggcgatga
     5641 acgacaggct ggcggccggg ctggccgagg accaactgct tgccgcctac gcgtgccgcg
     5701 acagcgagca cgcgctggcc gacgcgctgt gcgtcctggc ggatgagcac cgagccagcg
     5761 atagccacgc tcgggcctgc gagttgtacc agcatgcctt gagctactgc cccggcagtg
     5821 cccggacctg tgacctgatg accggcctgg cccgctcgag cattgccctg gcggactacg
     5881 acacggcaca acgcatggtc aatcaactcg caaccagcta ctcggctcaa gccgatttca
     5941 tccgccgcat gaacgagatc gcttgggact accatgtggc caagcagaac gagctggccg
     6001 agggcatcta tcggtacgtg gtggacaact tcggcgaccg caaggaggcc gtcttctccc
     6061 aatcggcgct ggtgatcgtt aagtatgtca ccggccagga cgaggtggcc gccgccgagt
     6121 tgactcgact gttcgacggg ttcccggaca acaagaggaa accggcggcc ctgaaacaag
     6181 tcgcgcaggc ctgtgagggt ctcaagaagt atcggcaagc cgcgcaagca taccggctca
     6241 tgctccagtt gacctccgat ccgctggagg ccgagcgcgt caccctggat tacatcggcg
     6301 cgaccatgcg agctatccag acgcaggaag acgcggaagc tctgctccag tactgcctgg
     6361 aagtcctcaa gacacggccg acgaacccga ccctggtgca tgacgccgtg gggtcgccgg
     6421 tcaggggctt cgggagaatc gccatcttga cggggcgcaa gagcctttcc atcgaggccg
     6481 ccgaccgtct gatggcggcc ctcgatccgg acgctcccga gtaccaacac gcactggccc
     6541 ggcacgtgcg agcctgggcc ttctcctgga accgccaggc agaagaagcg atccggcaaa
     6601 tggtcaacct ggcagacgac tacgcgggaa cgacggacaa gaacgtcgag cgactctgcg
     6661 ctacctcact gggccatgcc tacggctatg ccctggcaaa tctgggtcgg cccgatctgg
     6721 cgcagcagta cctcgatcag atccgctccc gctggcccga cgggcgacac gagagcgtga
     6781 tcgagctcta taccacacac gttccggcca gcgtcaagaa gctggccgag cagggcgcgg
     6841 cgatcatgca aggcaagccg gagcagtagc cgccgaagcg accggaggcc tgtcgggcac
     6901 ctccggaaaa cgttagacgt gcaggaccac aaaggcagtc gtgagaggaa cgaaccgtgt
     6961 acacgcatgc ttccaccgtc ggatcactcg gccgtgacgc cggctcacgc agagcgcatg
     7021 ttcttcccta tgtgctgatc gcactacttg cctgctggca gtcgcccgac caggccgccg
     7081 cgtgcggccc ctgtttcggt tacttctgtg gcgagtgcgc agactgtgat ccggtcgaag
     7141 agcgatgtat ccccaatcac cactcctgct gtccgtgttc ctactggggc acctatcggt
     7201 acgtcgatat ctgtgtctgg ccggacctga cctggctcgg tgatgtcgtg cgcgtccatg
     7261 tcgtcggatc gagcgcagcc ccagccgtct gctaccaact ggagcttttc tacgactcga
     7321 gatttgtgga gaacgacatc gcctgcccat tggagtatcc tcaggagatc atccagccga
     7381 ccttgccgtc gtaccaaagc tgtgcgggct acagcatgcc gttgccgagt gatccctcga
     7441 agggatggct cgccctgcct ggcagccatg atttctacgt ccaaccgatg cggcccaggt
     7501 tccaattggc gcgtctggaa gcttcgccat acaaccccta taccactgca tcgcggtact
     7561 tcgccgtcgc agacctcgat cttcgcggcc catataccct cgaccccaca tgccaccccg
     7621 gaagcccctg cgggtgctcc agcgggaacg acctggaacg atgcgagcac ccggatgact
     7681 acccgtgtca acccggcgac cctgcgatct tctacatcgg tgtcaacgac gatttcgacg
     7741 agggcaagga aacggaggac gccgaggacg tgagcattct cgacaacact tcacaacccg
     7801 cactgaatga tttggcccaa atgtgctgga ccatgggcac cgcgactgtg tatgaggcat
     7861 attggagtgg cacttacgac gtcatgtgcg gtgactcatt tgaggagagg gtatccgcca
     7921 agatgaccgt gtctcccggc gaaatcgtgc ggctttgggt gtggcacaac agccagtacg
     7981 agcaactgct gctcgggcag aacatcattc ccggctacgg aacggattcc tcgttctacg
     8041 cagaagggct caagcccgga acggtcacca tccagctcga gggcaagatc gacaacgacg
     8101 acagccggag gcgaatcgac aagatccggc tttctgcggt caaaatcgac gtcgacgtgg
     8161 atagcgataa taccaacacc acggccccct tcggcccgga cgcccccccg gcaacgcccc
     8221 cggccgccaa cgttgaagac cggatcgaac agaaggccgg tgagccgggg cggtttgtcc
     8281 tggttaacga cgacgacgac gaccaggacg gcatcctgga ctacgacgat gggtataata
     8341 aggacgagaa tcctatcttt ctgggtgatg acgtgacgta cgaggaagac gacttcgtca
     8401 tgctcaaggt caagatcgca ccgacgagca tcaacctgga ccccaaccag gcgtgggtgc
     8461 tgatcgacta ctcagcagac agcccggacc ccaacgtgtg gagcatcggg aggattcggc
     8521 tgtggaagaa gcctggcgac gagcctcgcg accggcgcag tgccctgacg accggccagg
     8581 gtgactggat agaacccggc ctctacacag acctgaaaac gctcatgcca tgggacccta
     8641 acctccagcc ggatcccggc aaccctccgg ccagccacga gatgactttc tacatcgaag
     8701 gtgtcaacgc ctcggggtcg ctcggcgaca cggacatcac cttccggatc gaccccgatg
     8761 gcccaggacc ggccggctgc gtccatgcgg ataccgcgca ggtgacggtg gtcagaccga
     8821 atctgggtgc ggactcgaac aatgacggtt tctacaacgg ggcggacgag atcattgagg
     8881 accggtcgga tctgtcgggg ctttacgtgg gcatgaacga caatttcgac gaggaggacg
     8941 atctggaaga cttcgagacc ccgcaggcct caaagaagcc ctgtcctgcc ggtcagtacg
     9001 ctgtcgttcc taccaacgat ctcgaaccag tctatgtgtc gctcgtgccc gggggcgaca
     9061 gtttctacgg agagtgtgac ctctacttga agggtggccg cgaccatctg cgggtgtatg
     9121 ccatccgttg gggaagctgt gacgccgcct gtccggcgac gggcgagaac gtgtgctgga
     9181 agctgatcga cgagaactac gatctggctg cgaactacct ccacagccag ggcccgattt
     9241 atggatgggc cctgtacgtg gagggcatct tgcctggcga agcacagatg ctcctggaat
     9301 tctggcacga cggccacaag ctgtacgagg actgggtcaa tttcacagtc gtccagatcg
     9361 acgtcgacgt ggacagcgat aacaccaaca ccaacgcccc ctatgggccg gatgcggccg
     9421 acccgatgac cgaggatcgg atcgaggaga agaacggtga gccggggcgg tacgttctgg
     9481 tcaatgacga cgacgacaac aagaacggca agctagacaa ttccgtgtta gaagtcgggc
     9541 cgattcagga cgaggacgat cttgtagaaa tcaaattcac agctccggat ttcacacccc
     9601 acaaccctga ctacgtcaag tggttgctga acgtgcctgc gaacatcgac atctggcaga
     9661 acaagaacaa aagcggtttc catgtatggg gcggagatgt cgctgaaata ccgtggacgg
     9721 atcatgcttt gccccagacc tggtacatgc agggcctgga ggtcccatcg agcggcccag
     9781 ttgaaatcga ggcaagcttg tggatcgatc cggacgagga tgggccgaag tcctacagac
     9841 tcgtgcatcg ggataccgtg caggtgacgg ttattcgcgt gggtctggat gttgacaccg
     9901 atcgtgatgg caccgtcaat cacttgcttg atgaatcgcg caaagacgag atcacccgtg
     9961 acaggggtgc actgttcatg accaactacg acgacgatga cgcgaacggt caccctgacg
    10021 cggtcagctt caacaacgaa ggccagccca ttgacgagaa cacggatatc gagcccggag
    10081 acgtgccgga catcgggcat cttgtcgtcc gggctccggg ttttgccacc accgggcttc
    10141 aattcttcct ccgcatggat gccgacgatc tcaaggcggt ccatatcttt gaggaacgcg
    10201 ccgaaggcaa acacgttctc aagacaagta gcggtgcgga ctggcctggc tggaccgctc
    10261 agagcagtgc ttcgggagac gcatccttgg atatcacggc cctggtgaat ccgacgctag
    10321 atgtagactt gggtatcgag gccctgttcc tgcgggggca caagacgagg tctccgtcct
    10381 acgagtttga cggccgggtc aaagtcgagt tggtggtcca actgaccaca ggtgcgacca
    10441 cgatcaaccc cgccaagatc ctcaaggtcg acactgtcca gctggccgtg gctccgtacc
    10501 tgctcctgcc gaacacgcag gcttgtcagg aggtctggct ccacacctgg acggaggcta
    10561 atccaatcgc gacggcgctt ggtgctctgg cgcatcgcgt ttcgtccggc ggtgggcagt
    10621 ggctccagga tcatgttcag gttgggatga cctcgattcc cggattgcag atgcacgtga
    10681 ccatgcggat gccgtattat cgagggcagc caggctggcc cgccagcagc ttgctgggcc
    10741 cccaacgggg ggtataccgt ttcaggaata cctgtaccca tgacggggcc gactctggcg
    10801 atttcggcgg caacctcgaa atcatcccct actcggacga ttggccgctg gggcgcatca
    10861 tcgtggggaa cacgatgagt acccgactaa gaagtttcct ccaagctcaa cgtatcgccg
    10921 gctccacggc cattcaggag ccgatcgtcg ccgataccag ttggctggcc gtcggtcaca
    10981 tcgacgagat catgtgtgcg gttcccggcg gggcccgcgg atataagatc gtgatggcca
    11041 gcccggacta cgcccatcag ctcctgacga cgggcgattc tgcccgcggc atcgccgctc
    11101 cgagtgagga tgcggccgtg ttcgcgaccg gggcacaggc cagcggaacg gcgtcgaacg
    11161 cgataacgac cacgtcgatg gtgtggctct tcgacggagc ggcgcatggg cgggtccagt
    11221 gcgtcgcttc cggagacatc tcggatggcg agactgtgac cctctccgac ggcaccacga
    11281 ccaaggtatt cgaattcgat aaggctggtg acggcgtcga taccggtcac gtgcaagtcg
    11341 atattgtcag tgccggcacc gcggcggacg tcagagacgc tcttcagtcg acgattgccg
    11401 cggccggcat gggggtcacg ccatacgccg acggttccga cgcgctgatc gtcatcaaca
    11461 acgcgctggg aacggcgggc aacgtgccca ttaccgacac agtgacggcg tcggctttct
    11521 cggtacaggg catggccggc ggcgaagctt ccgcgagcgt acgcgacttc aggggttcag
    11581 gctgggagtg ggtgcgtctc tttagcggaa cgggagcggg gcaggtcggg cacgtttccg
    11641 gtcaaggaat cgggtggatc agtgtggcgc aggtattcaa caccacatca cagatcgcgt
    11701 ctgctacggg tatctacggc ctcaccttcg ggagcgcgca tctcgcccac caacccagtg
    11761 gctggtttac agagccaggc tccggcactc gttaccttat cgttaccaac tcaaagaact
    11821 ggacaggcta tgatggaacg agcgaaattg atttcccggc cgcaatggcg gtctatgaag
    11881 ttctgcatga cactgacctc tggtctctga atacggccgc tcaaggcagg atcgaggggg
    11941 tccgcgctca aatccagact tccatgggca gcgggtactt gctccacgac accaccaccg
    12001 cggacgattc actctcgggg gacagcgatg atgacttcat tgctcttcct acgatcttct
    12061 ttggtccgag cggcacgttg gccgcccgcc acaacgcggc gttcacgccg ggattggcga
    12121 acgggctggc agcaaccggc tcgctcatcg tgtacccaca gcagtttggg gccagatccg
    12181 gagcggtgga tgtgtttgag gcagcaacta ccgctattat gggcagcggc atgacgcact
    12241 tcgctgatga ctgggatgga tatcataggc ttgaaggcga ggtccattgc gcaagtggcg
    12301 aggtcagaac gttgttctcg ttcaattggt gggagcatca gccatgaaat cgattccgag
    12361 gtttggcaat cagtcgttct ggatggcagt ggctcttcta gccggagggc tccgcatcgc
    12421 atgggccaat tcgagcgcgg aagaggttgt acaccaagcg gtggcgatct gttgccaggc
    12481 gcccacaggc aaagctgtgg cagacaggcc caacgcttgg ccggacttcg acaagctaat
    12541 agcggggttg agcaagtcaa tgctccccga tctgcgggca atcgccagtt ccgaaacaca
    12601 tacgaaactg gaacggcgag tcgcacaggt tctctgccgg agaattgagc accccgagga
    12661 gtttcagaag gtggagcctt ttctgacacc agtcgagaat cctacagggc ggatttacgg
    12721 accattggcg agcggaaagc gaagaccaag cattgcactc aagccgatag acgacccaga
    12781 gctgttagcg ccgcaggtac gggctgccat cgagagaaac ggcaaactct acgaagatct
    12841 ccgtacggag gctctgcgga tacgccaacg aatcaactcg ctcccggatg gggacatgcc
    12901 gtctctgaat cagatttacg ccgacctgga cacccgcatc gcggaattga agcgccagta
    12961 ccacgtggag ccggggcaaa ccctcgagct tgtgtttgcg tgcggcgatc cgagacaacg
    13021 gccaccgctg gatccgaaat accttccagc gtgggaagag gccgtaacca gggcatcttc
    13081 tcccgaaatg aaggcgaatc ttctgcaagt ggttcgctcc ttcagaagca gggactcggt
    13141 tccagtggta gccgcttgcc tgtcgacggc gagcaagagc ggtgacgagg gcatactctg
    13201 ggcaagcctt gcgttcttgc tggaggttga gcccaccgaa acggcagtgg acgaaatatc
    13261 gacagcgctc gacgagcttc agggttggat gcgttccagc gctgaacagc aagtgcgatc
    13321 ggcagtatcc cggtcacctc gctggcagac gtttctgcag caaatgaaag ctgatccgaa
    13381 gtggtcagcc aaggccgaca gactcctgcg acagactgcc tcgacaactg caccgggcga
    13441 ctgattccgc ttgccaggca ggacaatcgc cctcagagat ccgcggcaat cagcagaaag
    13501 gcagtctgcc gcccgaggga gcctataact cctccccgac ggagggttat tcccacgacc
    13561 ggagcatctt attcgtgcaa tctgctaaag gacgccctct tgatccgcgc cgatctgcgg
    13621 gccggtgagc cggggcggtt tgtcctggtt aacgacgacg acgacgacca ggacggcatc
    13681 ctggactacg acgatgggta taataaggac gagaatccta tctttctggg tgatgacgtg
    13741 acgtacgagg aagacgactt cgtcatgctc aaggtcaaga tcgcaccgac gagcatcaac
    13801 ctggacccca accaggcgtg ggtgctgatc gactactcag cagacagccc ggaccccaac
    13861 gtgtggagca tcgggaggat tcggctgtgg aagaagcctg gcgacgagcc tcgcgaccgg
    13921 cgcagtgccc tgacgaccgg ccagggtgac tggatagaac ccggcctcta cacagacctg
    13981 aaaacgctca tgccatggga ccctaacctc cagccggatc ccggcaaccc tccggccagc
    14041 cacgaaatga ccttctacat cgagggcgtc caggcctcac aggcccttgg cgacgcgaag
    14101 atcaccttca tgctggaccc cgacggcgac ggcccggccg gctacgtcca tctggatacc
    14161 gtccaggtca cggtgatcgc gttgacagcc agcccgggct ctggtcccgt cggcacgcgc
    14221 atcgatttcg aggcgtcgcc gaccgggctg ggcattttcg acggttccga ggcgatttca
    14281 ttccgaggga ccttcgagcc gaccggctac tgcaagacat gtgagttcac cagcgagcat
    14341 caggcgcatt tcgactctgc ctatccggat cgagtgtcga tcatcctcgg cgaagacacc
    14401 tccagcgata tctgctttct gttcaacctg ggcgtgatct tccgggctcc cggcgtgctt
    14461 cgcggcgaca ttgcgatcga gacggcctcc atcaagtgcc gcatccacgg cctcgccttc
    14521 agtgtcacac cgagcgccgc catcggagtt ctcgatggct caacgttctt ggaaacgtcg
    14581 gaaactgaca tcctgcacgc ggaaacaggc gaggatgtta ccaatgcccc cgcaacatac
    14641 cttcacgacg ctcgggtgct ggtgccggtc aatggaatca acgtgaaaag cgatcagctc
    14701 ccggacgcga cgacggaggt ggccatcaag tcgttcagcg tcttcggttt cgaacagggc
    14761 agcgaggccc tgaccctgcg acttgaaccg cctgggacac tacagagtac gctctacacc
    14821 tatttgaaca caggcgcagg tcggaggcgt atcatcttcg tggacaaccg gtcgctcgag
    14881 ggagtctact cggatcgtat tgcggtatac gcgccgttcc ctggtaaagt acgcgtggtc
    14941 aagtgatggt gaatgcgatg agatgtatcc cggtgtttcg tctcgcggtc agagtagtcc
    15001 ggtttggcgt agccccgtcg ggtgtcttgc tcctgctcgc agcagcactc tccactcttt
    15061 ccgtcggctg cgacgggggt acggagacga agcgggcgaa cggtccgcct ccccgcgtgc
    15121 atttgatgaa gccttacacc gatggctgga ctgacagtga catctcgcca ttcggtgacg
    15181 tcgtgttgaa ggaccaaaaa ctgcgcatac gtgtcaagtt ggatcaggcg gttcagcaga
    15241 aatctgacat aaccgttgag ataaccttcg acaatcagag ctggcagccg ctgagcctca
    15301 aggacgccga tgcgcttgtt gacgccggca aggaactgcg tgtctccgtt ccctgggtgg
    15361 acatgctgag ccaaggttgg actgccgatg gtgaggatgc cgtccctgag caggcaagtg
    15421 ctgactcgtc tgctgacccg ccattcgacg agacaccccc tgagtcgggg gactatccga
    15481 acaatccctc gtccaatcgg gcagattcgg atgcctttga tggctgcggt ctggcatctg
    15541 ctcaacgggg gaaggcacga ggcccgggca acgagacgct gtggcccccg gaatcggtaa
    15601 tcaacaaaca gtttctcgct tccgcaggag tggtctactg taaggtgcgg gccacagcgc
    15661 tgaatgcaac tgggttcgcc aacgtgccgg gccagctcca aaaccaggcc gactggttct
    15721 actactccgg gcacggattc aatcggcgtt acaccggcga cggggttggg cgactgcttg
    15781 ccgcggacgc gcaaaatggt ttagcggcgg cggacgccaa gtggagcccg gacgtgaagc
    15841 atgtcatcat cgcgggctgt tccatgatca atatcaacga tcccaaccgg gattgttctg
    15901 cctgtgagga cgatggaatc ctatgggctc agaccggtcc aacgcacatt gcagggtaca
    15961 actggaaagc gccgtctgac aatgtctatc ctgggccgaa caactacacg gctcaaatcg
    16021 ttggtcattt ctgtacccgg ctcggcggtg agtcctttgc gacagcatgg ctgaatgcga
    16081 acgcagacat ggatcctgac aaactccacg taagggcgag cccggatgaa cagggaggac
    16141 caggccgcgt atggaacgcc gccgcgatag atttgagcgg agggccggga aatggtgtat
    16201 actactactg ggatatcgca accaaccctg cgcgaccaga gatcaagatg attccgcaga
    16261 gtcaatggta ggcgccctta tgagaagacg aaaggcgatc cagatgagcc gtgtcctggg
    16321 aatcctatgt gtcctcgggg tgtccggccc cggaatcggc gccgacccta aaatgcctgc
    16381 agagcccacc tctggagtct ggatcgaccc cgcgagacct tacgaggtga gcggcaagct
    16441 aactctcgta gtggctcagc gagttgaagc ctctggagaa gccaacgaca gactccagaa
    16501 ggcggtggat ttctaccagg catatgtaaa gacatccgct gagatgaatg aacgggctgg
    16561 gcccaaccgc gaactgaccc ctagcgagat gcagcgtgtt ttgcactcgc gacttgtggc
    16621 aattgagcag ttcgttgaag tgaacaaggt gttcccggag acctcggcag ggctgacggc
    16681 gatcctatgg gtcggaagcc tggtgcatgg tcagagttcg gaactggccg cctcggtact
    16741 cccggccctg cgagaactcc ggcaccggca cggcgcttcg tggcagggtg ccctcgcctc
    16801 tcacgtcgag tcaccgcttc tctatgtttc tttgccccca cagggaaccc cagacgcacg
    16861 gcgcagggca ctttatgagg cgcagtacca gcttatgctg gagactctgc ctaagggcga
    16921 ggtgtcagtc gatcgaacgt tgcccggcgt ggacctcctg ttgagggatc ggctagcggt
    16981 gaaaggctcg atccgagcgt cattcctcca gtggatcagg gcctgtgaat acaacttgtc
    17041 ctatctgtcg cgaggcgaag aagcgagaga atggctgcga aagagcatcg cgacgaacca
    17101 gcggatcatg actgagtatc ctggcaccga ggaatcgaaa gacgccgacg gtcggaccta
    17161 ccagtttgcg aggagggcag ccgaattcgc
//
LOCUS       DAOTPW010000117        17106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3336_length_17106_cov_5.959826, whole genome shotgun sequence.
ACCESSION   DAOTPW010000117 DAOTPW010000000
VERSION     DAOTPW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17106
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3336_length_17106_cov_5.959826"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="P5316_11865"
     CDS             complement(<1..462)
                     /locus_tag="P5316_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85695.1"
                     /translation="MGVTQRRVSRCITVVAVVAGAAPAWGITYDLPFHEDFEPTPDHW
                     NWTAEWIACDHPGNRTTCPDDPVLLPCDWTRMNEFSSCWGPYKFESWPDAANGGIVFS
                     GLRSGRQPIADPYWHTILHPFTPPVGVGDLRLTLWQYDPAQILCDCDPWGPP"
     gene            complement(506..1498)
                     /locus_tag="P5316_11870"
     CDS             complement(506..1498)
                     /locus_tag="P5316_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85696.1"
                     /translation="MINGYLLQPLIGSDPGHQKNHTPSNDGQMGDPSIGGAAFEVDNN
                     PYPYASYHEFEPRTGILRLSAWVWEDAERPLCTPSTWPVRGMVGLTSLPGEPVITRIG
                     DRVYHEIYPYEDYAFIGVESPTSNNPSDPIQKFYRWRTKSDGWHLTDVPRKADYVCHG
                     AQTWRHVEIVVYPYTGRVGDIRFFIDGVLVGEGRRQGGTDCRGVDLVRIQLGTRFPET
                     ADGTSYKPPYSYEHFWFDDIALTVDTSSLPCVNPQLRFDADGDGDVDQADFAVMQACF
                     TGADGGPFDCLTCRCMNSDGDTDVDGDDMAAFEQCVSGPGVVADPACDDGLAYP"
     gene            complement(1905..2585)
                     /locus_tag="P5316_11875"
     CDS             complement(1905..2585)
                     /locus_tag="P5316_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85697.1"
                     /translation="MRSLFMIALILACLATATMGAVGETWILPIHHRDGGGWTEVPGA
                     GYGGASAWQGSGIDGVRRVYWELSGIGSMGNPVPSTTELYTIEAFTPTSWPGRMDWQP
                     IESQIKGVDGETWPMESLIPWVGAWGTNHQYVGSNAGAAGTWVSAGPGPHSPESADYN
                     AGASGIYMWLGGHGSPSWLYAKWDFGWSIERTWSALRVTQITPEPASALLLLLGAPML
                     LGRKRNPL"
     gene            3214..5112
                     /locus_tag="P5316_11880"
     CDS             3214..5112
                     /locus_tag="P5316_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85698.1"
                     /translation="MLGTWSRVVLLIWASPVLAQWQLVSPHDRRESAPPAAGTGSPAI
                     RPGDSIPHDDKFRWLIADLAVPETIDDKAAADLPVGLQISAGDGGEIWVDGRLKVRYD
                     NDHPGLVVISERAVPGTTVHLAVQVYAKVQGGDTFDEARWVLLDPKRVESRLVLTANP
                     RRLLGDVPNGIVGLSQGGGLADYEDATARKLREGGFKWFRMDNILTGVVRHADDGALT
                     YDWTDFDRRVDFIVDKMKADPIFAVSYMPLPFDAVRNDDRQSAPKDYSLWEDLCYRAA
                     KRCIERGKRVPFWEVWNEVNSGWLKPGPDDKGDHPFRDIYKQALDGQDGEAETVRRFE
                     AYCKLYRATARGVLRADPKAAIGGPALASGPFESKQFGHCANGKGFARGLMLWCQKER
                     LPLDFVSWHEYFHPADVIRGQADAFRKYLADFPELKKTVRSFMITEWNQAWWADRPQD
                     HEIGAAYCADCIVRAFIPGRIDHPCFFYVKQNDMNFRGDFSLLMADNVPKASYNVLKI
                     FNRLSGRWIELSGGDGEVAGVAAWDETQGKLAIVLVNFQYRHGIRRHVRIRVEPLPDP
                     LNNGTWREWIVDATRSNIWNDPGRPQKAELTESSHGRITTAKLELDRTLEPNSVTLIE
                     ALRPVEKP"
     gene            5501..7363
                     /locus_tag="P5316_11885"
     CDS             5501..7363
                     /locus_tag="P5316_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85699.1"
                     /translation="MRYPIPIAALAVVALFVVPAAAQTIEAEMILEAFDGCGCSPPSP
                     PLCSEPHQLKWPFKRDWWGYQLNTTGPTVAGVMPSGWSHITSLGSIEVTFSEAVTGVT
                     AGLLEVHDENESWTATSVSGSGAGPYIFTGPWNPDDEVVRVELNAGSIGGPSGNFGGH
                     FWTYVKNRPDWVTVTSVMPARASSIAGLDSVQVTFSTEVQNVTAGHLTVNGSAATSVT
                     GSGAGPYTFTGYNPPSNGTVFVQLRPESFKDNRPTIRAAAPIDCNCHDAASNDPSVAG
                     CSAGSAWPHPYPNGSFWSANDKIDQDWSSQAFPLNFLGTNQIKNGSELDYPPNQGSQG
                     FQLFDTQRTLYNSGEFGALGYINPDNPQDRGPFGSGRIHVDQIRAEFNARFPDVPLPE
                     PIPGQPAYLEFDVRRDQNNDLVIVLEAYAGGIRPSGVTFSLTPEAERTVIPDDPGNWH
                     TYRIGTFSSFPDSFRFLIIMWLRNPTHPAGYPWTAPGDVQCYIDNVKLVYRAEPFPEN
                     CSNKHDDDGDGLIDCEDDDCYHDAACPCNELLVFDVDEDGDVDQADFSLLQSCLTGTG
                     DPGGAFRSLPEACRCLDITGPGNEPDNALDQQDLVAFELCASGPGIPANPACDD"
     gene            7488..9038
                     /locus_tag="P5316_11890"
     CDS             7488..9038
                     /locus_tag="P5316_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85700.1"
                     /translation="MVIDRLHLLAWTLLVVAPTSQAAEPEHWRFGVGVATQNMIDGQT
                     TTNLEDFDSYRPADEPWNVLGIGWYFNWNWRQGIVCDASRNRCVEYMPLVGGWGPGVH
                     PTLSQIADHLNANPGLYPDGTTWLIGNEIILDDRRTPLQYARDYHTYYHGLKAVNPTF
                     KVANGSVITSIYYNYTGFVGTPYELLDAVWNAYLNEYGETWPVDVWNIHPYVWTKPTL
                     KAKLDDFANQLSTFRDWMAARGQQDKALIITEYGLLNYHEPQWMIDYLLGSFEILLST
                     GHGNGMTSDEGRWVQRWAWFVNNMHVWTAGGAIQWEHCALHDAEVDLDGDGEVFDLRP
                     LGRAFADHPKFDNCPSTWNPLQQDVDGDERGDACDNCPLLPNADQADADGDNWGNVCD
                     GCPDDPFKAVPGFCGCGIPDGDADGDAVMDCVDECPDTPPGSRVTLNGCPTARADLDR
                     DGDVDQVDFGLFQACLSGAGISAADMSCAAASFDEDDDVDSDDCAVFRLCLSGPDSPS
                     DPACGRSQ"
     gene            9144..11339
                     /locus_tag="P5316_11895"
     CDS             9144..11339
                     /locus_tag="P5316_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HRR85701.1"
                     /translation="MRMGLLAGMGLLLARLATGTGDAATINIQSIGLSGTQIELYQPL
                     TVSFNLSKSYPNPYDPDTVDARFEFTGPSGVTRVIPAYWTEQTPRWRARIVPLETGPH
                     SCRIVVNDSAGDSGSATLSFMGVSSSRRGFVRIDPRNPRYLRFDNGEPYLPIGHNVCW
                     YGWDPLFSQWLDRMSQVGENWTRYWMVAYVSQDIEWGGGTSFQLGRYSQSNAALIDGM
                     LEAARARGIYVQLCLDSFNGWNETLYANWADNPYNAANGGMCTRPNDYFVNAEAKRLA
                     KRRFRYIVARWAWNPAILCWEFFNEVDAVGGGGATFWGHEAEIAQWHQEMAQYIRSID
                     PFEHLRSTSFADDGPRSSYAPFWQLPEMEIVQVHQYSTVLPQSHVNLIRQVRQFGKPV
                     IMGEGDYAGGPDSLDPNGQSLHDMIWAAAVVESGAMSWWWDNWIHPNNLYYRFEPLVA
                     FLSSQDWAPQQLSPLAYTILSGHTTPEIYGSGGSRHGYLYIRNWQGPVSGLRLRLNQI
                     TSPGNYVAEYWDTNSTTPFRSEIIPGSPSGLELNIPDFARDVAAKLKQTGPVLSAVPT
                     LLTVSSFIGHDARPQSFAIGNTGTGSTNYEITVDQFWLSVSPSSGGIEQETDIIDVAY
                     QTAGLPLGLYTASITVSDTGGHSPPAVVSVTLQVRPLPADLDTDGDVDQEDFGRLQVC
                     LSGNGVVQTAPECDLGRLDNDGDVDRDDVTLFLGCISGPSVPADPECVK"
     gene            11679..12956
                     /locus_tag="P5316_11900"
     CDS             11679..12956
                     /locus_tag="P5316_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR85702.1"
                     /translation="MSYIRILLLGGLALWTGCSAPTTIGYPAVSPDGKGFVSGGTSFL
                     VWGFNYDRSVISGRDVLLEDVLREQPAKLDRDFAMMRRMGGNTVRLFCQMGEYFERPD
                     HINAAAFERLSMALDAADRADLKVLLVGLCHIRKSTIPVWLVEADDDTVERHEELFWR
                     QTAKRFRDHPAILAYDLQNEPAVHGSDSRDLVVGCFSMRGGEQFCYVHMHGRQASRRW
                     AEWVHGRFGTEAAVRAAWKDYPRPGENWFSPALPKTSREDPRLGDLLACHRDLLQAWA
                     QRMRSAIRENDGNHLITVGALNPAVFADIVDFHCAHLYPKQDLTLEANEQAWRDHLSR
                     LPPGKPIVVEEFYPLAYPHRADGQAATATELIAAVRRAAGPHGAGFVSFYWGPAEEVI
                     ESPMGQALYQNWLDAWSSAGRVASGTPSTTHSR"
     gene            complement(13086..13496)
                     /locus_tag="P5316_11905"
     CDS             complement(13086..13496)
                     /locus_tag="P5316_11905"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR85703.1"
                     /translation="MSTGLVAEYMTREKWREALFTYKERDSSPSYAKGRRKHLRFRMA
                     GWATVFIDSADKQAAQTLQGCQLLDVSEEGMALRVPRKIEPNTSVTVELHVSGRIFRL
                     SGRAIYHTRLPGAVRVGIKLEFGSGDKQAHSTDR"
     gene            13966..14038
                     /locus_tag="P5316_11910"
     tRNA            13966..14038
                     /locus_tag="P5316_11910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13999..14001,aa:Ile,seq:cat)
     gene            complement(14288..>17106)
                     /locus_tag="P5316_11915"
     CDS             complement(14288..>17106)
                     /locus_tag="P5316_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRR85704.1"
                     /translation="GLGRGGGEIECRLRSRDGHEFDALIWYNPIYHEGLTQMVGCTFT
                     DITRRKQAEAELKKAHQLLQERFRLQGDELLRASEQLEQRAQEQDLTRQALDRQRNLL
                     QTLIDHLPDQIYVKDRESRFRLVNRTVLSRFGLSNLSELIGKTDADFFPPDAAQQYLQ
                     EERHILETGEPLVNVEQEARYPGKAPVWTLVTKVPLRDERTGEVVGFVGLNRDITHLK
                     QMEQEMLAAERQHRAVLDAMTDAIHVIDRDYRIVLVNQAFRNWIERLGIRAEPVGRPL
                     TEAVPLIPESSLDHYKQIFNSGESIEYELETRVQDTSVVLEIRKIPIMESDQVVRVAT
                     ILRDITERRRVEESFQRSEARIRQMVESLPIALSSVEADTGRVLLLEGDTEGILGYDH
                     AQIFSDPHLGAAIVHPEDRRATYASFEEGISSRKPFSMTYRIIHGKRRTPVYLQRYVV
                     PVHDSNNRLVRHDSIIIDVTAEREAQQRLKLLSQVVEQTSEGVAVVDLDGKVLFLNRA
                     CAAMHGGGDADFLGKPLSVFHTSEQAPAVYQAIRQTVTKGEFEEETGHVRKDGSTFLA
                     SMRSTLLRNESGAPMGCIVLMRDITAWRAMQSASVRLAEAVKNAGEGIAIWDGSWHLT
                     YANASMLAMLGCSSLEELEVLKAFRDSIRKGEGHGDNVLAGLHKTGVWRGRLRTERKD
                     GSVVSLAATFSRLVLDDGGELVVGNFTDMSREEAQLEQIRRLGREAAALLDKERARLS
                     RELHDELGQQLTAMNLALAWLESHLAAVEPPIRERLSEVRDLQTRMTETVRNLAKSLR
                     PAVFDYQTLPEALQSLITEYGQSGGLTCRVKAEPPNLNVQNPLKTTIYRITQEALTNV
                     LRHSNATRCEVKLRATPRGIELRVTDNGTGADPSKLEGTASLGIVGMRERATALGGEL
                     RVESLRRGGVRVLARFPWPRNHQP"
BASE COUNT         3318 a         5473 c         5039 g         3276 t
ORIGIN      
        1 tggaggcccc cagggatcac agtcgcagag gatctgagca gggtcatact gccacaaggt
       61 aagccgcaag tcacccacgc caacgggcgg ggtgaaggga tggaggatgg tgtgccagta
      121 cgggtccgca atcggctgcc ggcccgaacg aagacccgag aatacgatcc cgccattggc
      181 tgcatccggc caactctcga acttgtaggg cccccagcaa gagctgaatt cattcatgcg
      241 ggtccagtcg cacggcagaa gaacggggtc atcggggcag gtcgtacgat tcccgggatg
      301 gtcgcaggct atccactccg ccgtccaatt ccaatggtcc ggcgtcggtt caaagtcttc
      361 gtggaacgga aggtcgtatg tgatgcccca ggctggagcg gcgccggcaa ccaccgcaac
      421 cacggtgatg catctggaca cccgtcgctg cgttactccc atcggcaaac ctctcaagtc
      481 ccctacgagc ctctgtgccc ctcaatcacg gataggccaa accgtcgtcg catgctgggt
      541 ctgcgaccac acccggcccg gacacgcatt gttcaaaggc ggccatgtcg tcgccgtcga
      601 cgtccgtgtc gccgtcgctg ttcatgcacc ggcaggtcaa acagtcgaac gggccgccat
      661 cggcacccgt gaaacacgcc tgcatgacgg cgaagtccgc ctgatccacg tcaccgtcac
      721 cgtcggcgtc aaaccgcaac tgtggattga cacacggcag gctgctcgta tcgacggtga
      781 gggctatgtc gtcaaaccag aaatgctcgt acgaatatgg aggtttgtat gacgtcccat
      841 cagcggtctc gggaaaacgc gtcccaagct gaattcgaac gagatccacc ccgcgacaat
      901 cggtgccacc ctgccggcgt ccctcgccga ccagcacccc atcgatgaaa aaccggatgt
      961 caccaactct accagtgtag gggtatacga cgatttcaac gtgacgccac gtttgagcac
     1021 cgtggcagac atagtcggct ttgcggggta cgtcggtgag atgccagcca tcagacttgg
     1081 ttcgccagcg atagaacttc tgaatggggt cgcttgggtt gttcgacgtc ggtgattcca
     1141 cgccaatgaa ggcatagtcc tcatacgggt aaatctcatg ataaacgcgg tccccgatcc
     1201 tggtgatcac cggctcgccg ggcaaagagg tgagcccgac catccctcgg acaggccagg
     1261 tggacggcgt gcacagaggt ctctcggcgt cttcccaaac ccaggcgctc agccgcagaa
     1321 tccctgtccg gggttcaaac tcgtggtacg aagcataggg atacggattg ttatcgacct
     1381 caaatgcggc tccgccaatc gacggatctc ccatttgtcc gtcgttggaa ggcgtgtggt
     1441 ttttctgatg cccagggtca ctgccgatca gcggctgtag aaggtacccg ttgatcattc
     1501 cagggaccgc tgaccaatgg gtccccgaca ctgcgccacc agggtcaccg tcgtcgaaat
     1561 catcattaaa gacaagtgtc tgagccagaa catgcgtcga taccggaccc gatgcgaagc
     1621 ccgaaagcaa gacagctatc acagacaacg atcctaaggc ggaccgcctt tttgcgggcc
     1681 gcaacccgag cgctctgcga tccggaaaca tgagcgcaaa atgcgcacca gtcccgatgg
     1741 aagagactaa cactcgcttc atggccgttt ccttttgtgc agccaacgtg ttccccacct
     1801 tctccatgtg agacgagggc agagcaggag cccgtcatgc ccccgctctg ccgtcattct
     1861 gttctggtca aaatcccgtc cgccgtggtg atgcctctgc ttggctacag cgggttgcgc
     1921 ttacggccca gaagcatcgg agcccccagc agcagcaaca gtgcgctggc cggctcgggc
     1981 gtgatctgcg tgacgcgaag ggcactccat gtccgctcga tcgaccagcc gaaatcccac
     2041 ttggcataca gccacgaggg cgaaccatgg ccgcccagcc acatgtagat gccgctggcg
     2101 ccggcattgt agtccgcgct ttcgggcgaa tgggggccgg gcccggcgga tacccacgtg
     2161 cccgccgcac ccgcattgct gccgacatac tgatggttcg ttccccacgc cccgacccac
     2221 ggaatgagcg actccatcgg ccatgtctcg ccgtcaacgc ccttgatctg cgactcgatg
     2281 ggctgccagt ccatcctgcc cggccaactc gtgggagtga atgcctcgat cgtataaagc
     2341 tcggtcgtgg atggcacagg attccccatg ctgccgatgc cactcaactc ccaatacacc
     2401 cggcgtacgc cgtctattcc cgaaccctgc catgcacttg cgccgccgta gcccgcgccc
     2461 ggaacctccg tccatccgcc gccatcacga tggtgaatcg ggaggatcca ggtctccccg
     2521 acggcgccca tggtcgcggt cgccaaacag gccaggatca gagcaatcat gaataggctt
     2581 ctcatagata cctcctcaca tttgaagaca ctcaggcgtc tgagacacga cgccgtttcc
     2641 agatccgatc ggcagcccac cccgctggtg gcgcttgtcc gaactctttt cttaggattt
     2701 cctggggcac ccaggcgtga gatacggtct ttcgccccta ccctcctgtg aaagcgcgaa
     2761 accggtcctc ccccatactc gtttgccccc ggggacgtgc catccgcaaa ggatcgcaag
     2821 cccgaggttg catcataact ccaccgtctg gaatggtcaa gacaaaatcg ttcacaaaat
     2881 gtttgttggc aaaggattgc gagcatccgg agaccgcgtt caatcgatct tccgcgttgt
     2941 tagctcgatt cactacgtcc agccaagaca acacacttta tcttttgcct gtgtggccgg
     3001 gcccgtcgac accggggaag gatcgtcgca tcttgcctcg attccgggaa tctcgtatct
     3061 atgcgaaccg tccgcaaggc cgttcagcgt tgctgggcca aggtggttaa tggaacctta
     3121 tgaccagaga tcggtgcggc cggggcaggt ttgcgcgctt ccgcgtggtt ggattgtggc
     3181 ccgcaagagg gcggtccgtt tggagagcat gaaatgcttg gaacatggtc aagagtggta
     3241 ttactgatat gggcctcgcc ggttcttgca caatggcagc tcgtgtcccc gcacgaccgg
     3301 cgggaatccg cgccgccggc cgcggggacg ggctcgccgg cgatccggcc cggcgattcc
     3361 atccctcatg acgacaagtt ccgctggctg atcgctgatc tggccgttcc ggagaccatc
     3421 gatgacaagg cagcggcaga cctgcctgtc ggactgcaga tcagcgccgg cgacggcggc
     3481 gagatctggg tcgacgggcg gctcaaggtc cgctacgaca acgaccaccc cggcctggtg
     3541 gtcatctccg agcgagcggt tccgggcacg acggttcacc tggccgttca ggtatatgcg
     3601 aaagtccaag gtggcgacac attcgatgag gccagatggg tgttgctgga tcccaagcgg
     3661 gtcgagagtc gcctggttct gaccgccaat ccgcgtcgtc ttcttgggga cgttcccaac
     3721 ggcatcgtcg gattgagtca gggcggcggc ctggcggact acgaggacgc gaccgcccgc
     3781 aagctgcgcg agggcggctt caagtggttt cggatggaca acatcctgac cggagtcgtc
     3841 aggcacgctg acgacggtgc cctgacctac gactggaccg acttcgaccg ccgcgtcgac
     3901 ttcatcgttg ataagatgaa agccgatccg atcttcgcgg tgtcttatat gccgctgccg
     3961 ttcgacgcgg ttcgcaatga cgaccgtcag agtgccccga aggattattc gttgtgggag
     4021 gacctttgct accgggcagc caagcgatgc atcgaacggg gtaagcgtgt ccccttttgg
     4081 gaagtctgga atgaagtcaa cagcggctgg ctcaagcccg ggcccgacga caagggtgac
     4141 catcccttta gggacatcta caagcaggcc ctcgacggcc aggacggtga ggccgagacc
     4201 gtccgccgct tcgaggctta ctgcaagctg tatcgtgcca ccgctcgcgg ggtacttcgc
     4261 gccgatccca aggccgcgat cggcgggccg gcgctggcca gcggtccctt cgagtcgaaa
     4321 cagttcgggc actgcgcgaa cggcaaaggg tttgcccgcg gcctgatgct ctggtgccag
     4381 aaagaaaggc tccctttgga tttcgtcagt tggcacgagt acttccaccc ggccgacgtg
     4441 atccgcggac aggcagacgc cttccggaag tatctcgccg acttccccga gcttaagaag
     4501 accgttcggt cattcatgat caccgaatgg aatcaagcct ggtgggccga ccgcccccag
     4561 gaccacgaaa tcggcgccgc ctattgcgcc gactgcatcg tgcgagcgtt catccccggc
     4621 aggatcgacc acccttgctt cttctatgtc aaacagaacg acatgaactt ccgcggcgat
     4681 ttcagcctgc tcatggccga caatgtgccc aaggcctcct acaacgtgct caagatcttc
     4741 aaccggctct ccgggcgttg gatcgagttg tcgggcggtg acggagaagt cgccggcgtc
     4801 gccgcgtggg atgaaaccca aggaaaacta gccatcgttc tggtgaactt ccagtatcgt
     4861 catggtatcc ggcgacatgt tcgaatccgc gtcgaaccgt tacccgaccc attgaacaac
     4921 ggtacctggc gagagtggat cgtcgacgcg actcgcagca acatctggaa cgatccgggc
     4981 cggccccaga aagccgaact gaccgagagc tctcacggcc ggatcaccac cgcgaagctg
     5041 gaactcgaca gaacgctgga acccaactcg gtgacgctga tcgaggccct caggcccgtg
     5101 gaaaagccct gacgcccgga agactcggat tccgacaacc cgcaacacaa gccatccatc
     5161 gagaggttca catacgtggc aacgattcgg tctttcccga ccgtcaagca gtcccgtctc
     5221 gagtgaccgc gaacacagcg tttgaaacca gccggcatcg cggaacggct gcaaaacgtt
     5281 gactccacga ccgcgttcga gtacactgta atcgcgtgga gacgtgttaa tcgcgacccg
     5341 gtggatcggc ggctgcggct ccagggccct attcaagccc agcccgaggg ccaaacgcaa
     5401 tacgcctcgc gagcccactg gcaagacaat gcagttcggg gtctccgcga cggcagtgtt
     5461 gataggcaca tcatcacaat cgtacgaggg agggcaaatc atgcgatacc cgattccaat
     5521 cgcggctttg gctgtcgtgg cacttttcgt cgtcccggca gccgctcaga caattgaggc
     5581 ggagatgatc ctcgaggcat ttgacggctg cggatgtagc cccccgagcc cgcccttgtg
     5641 ctcggaaccg caccaactca aatggccttt caaacgtgac tggtggggct atcaactgaa
     5701 caccaccgga cccacggtcg ccggcgtcat gccgagcggg tggtcgcaca tcaccagcct
     5761 tggctcgatc gaggtgacgt tctccgaggc ggtgacaggc gtaaccgccg gcttgctcga
     5821 agtgcacgat gagaacgaga gctggacggc cacaagcgta agcggcagcg gtgcggggcc
     5881 gtacatcttc accggaccat ggaatcctga cgacgaggtc gtccgagtcg agctgaacgc
     5941 cggttcgatc ggcgggccca gcggcaactt cggcgggcat ttctggacgt atgtgaagaa
     6001 cagacccgac tgggtcactg tgaccagtgt gatgccggca agggcctcgt cgattgccgg
     6061 ccttgactcg gtgcaggtga ccttttcaac ggaagtgcag aacgtgacgg cggggcatct
     6121 cacggtcaat ggttcagcgg ctacgagcgt gaccggcagc ggtgcgggcc cctacacctt
     6181 caccggctac aaccctccgt ccaacgggac cgttttcgta cagttgcgcc ccgagagctt
     6241 caaggacaac cgccccacga ttcgggccgc tgcacctatt gactgcaatt gccatgatgc
     6301 cgcctcaaat gatccttctg tggcgggttg cagcgcgggt tccgcctggc cgcacccgta
     6361 ccccaacggg agtttctgga gcgccaacga caagatcgac caggactggt ccagccaggc
     6421 gtttcctctc aacttcctgg gcaccaacca gatcaagaac ggcagcgagc tcgactatcc
     6481 gcccaaccag ggatctcagg gcttccaact gtttgacacc caaagaactc tctacaactc
     6541 aggggagttc ggggccctcg gctacatcaa ccccgacaat ccgcaggaca ggggtccgtt
     6601 cggatcgggg cggattcatg tcgatcagat ccgggccgaa ttcaatgccc gtttccccga
     6661 tgtcccgcta cccgagccga ttccgggtca gccggcgtat ctcgagttcg atgtcaggcg
     6721 ggaccagaac aatgacttgg tgatcgttct tgaagcatac gccgggggca tccggccttc
     6781 gggcgtcact ttctccttga ccccggaagc agagcggacg gtcataccag acgacccggg
     6841 gaactggcac acctatcgca tcggtacgtt cagcagcttc ccggacagtt tccggttcct
     6901 gatcatcatg tggctgcgca atcccacgca tccggccggg tacccgtgga cggctcccgg
     6961 cgacgtgcag tgctacattg acaacgtcaa gctcgtttat cgggctgagc cgttccccga
     7021 aaactgcagt aataagcatg acgatgacgg cgacggcctg atcgactgcg aggatgacga
     7081 ttgttaccac gatgcggcgt gcccgtgcaa tgaactcctg gtcttcgacg tcgacgagga
     7141 cggcgacgtg gatcaggcag acttcagcct gctgcagagc tgtcttaccg ggacaggcga
     7201 tccgggcggc gcgttcagat cgctgccgga ggcctgcagg tgccttgaca tcaccggacc
     7261 gggcaacgag ccggataacg ctcttgacca gcaggatctt gtcgcgttcg aactctgcgc
     7321 cagtggcccg ggaatacccg caaaccccgc atgcgacgat tgatgcgcag cccggaccga
     7381 cgatactgaa acggccggca agatcgagat agcaggtacc gcagaggttc aattcgatcg
     7441 gttgaggagt agggcttggc ggattctcga gaaccgaggc gcacaagatg gtgatcgatc
     7501 gactccacct gcttgcgtgg actctcctgg tcgttgcccc cacatcgcag gcggccgagc
     7561 cggagcactg gcggttcggc gtcggcgtcg ccacacagaa tatgatcgac gggcaaacca
     7621 cgaccaactt ggaggacttc gacagctacc gccctgccga cgaaccgtgg aacgtccttg
     7681 gtatcgggtg gtacttcaac tggaactggc gacaggggat cgtctgtgac gcatcgcgca
     7741 accgctgcgt cgagtacatg ccgctggtcg ggggctgggg gcccggggtg catcccacgt
     7801 tgtcgcagat cgcggatcac ctcaatgcga accccggcct gtaccccgac ggcaccacgt
     7861 ggctgatcgg aaatgaaatc atcttggatg atcggcgcac ccccctgcaa tatgcccggg
     7921 attatcacac atattatcac ggtcttaagg ccgtcaaccc gactttcaag gtggccaacg
     7981 gatccgtcat cacatccatc tactacaact acacgggctt tgtcggcacg ccctatgaac
     8041 tgctcgacgc cgtctggaac gcctatctga acgagtacgg agaaacctgg ccggttgacg
     8101 tgtggaacat ccatccctac gtctggacga aaccgacgtt gaaggcgaaa ctcgatgact
     8161 tcgccaacca gctcagcacc ttccgcgact ggatggccgc caggggacaa caggacaagg
     8221 ccctgatcat cactgagtac ggcctgctga attaccacga gccgcaatgg atgatcgatt
     8281 acctgctcgg ctcattcgaa atccttctgt cgaccggaca tggcaacggc atgaccagcg
     8341 acgagggacg atgggtgcaa cgctgggcgt ggttcgtcaa taacatgcat gtctggacgg
     8401 cgggtggtgc gatacagtgg gaacattgtg cccttcatga cgccgaggtt gatcttgacg
     8461 gcgacggcga agtcttcgac ttacgaccgc tgggccgggc gttcgccgat catccgaaat
     8521 tcgacaactg cccgagcact tggaatccac tgcaacagga cgtagatggc gatgaacggg
     8581 gcgatgcctg cgacaactgc ccgttgttgc caaacgcgga ccaggcagat gcggacggcg
     8641 ataactgggg aaatgtctgc gacggctgtc cggacgatcc tttcaaggcc gttcccggtt
     8701 tctgtggttg cggtatcccg gacggcgacg ccgatggcga cgcggtcatg gattgcgtcg
     8761 atgaatgtcc ggacacgccc ccgggttcgc gcgtgacgct gaacggatgc cccaccgcca
     8821 gagcggacct cgaccgcgat ggggacgtgg atcaggtcga cttcggcctg tttcaggcct
     8881 gcctgtcagg ggcgggcata tcggcagcgg acatgagttg cgcggcggcg tcgtttgacg
     8941 aagatgacga cgtggacagc gacgactgtg ccgtgtttcg cctttgcctc agcggtccgg
     9001 acagcccctc ggaccctgct tgcggacgca gccagtgacg caatgaacgc tggcagatgc
     9061 cgtggggtag gaccggctac tggagatcgc tgatccgctc aaggtgcaac gagtagagcc
     9121 caaatgccgg aagccaaata cgcatgagga tgggactatt agcggggatg ggcttgcttc
     9181 ttgctcgcct ggccaccggc accggcgatg ccgcaacgat caacatccag tctattggcc
     9241 tgagcggcac ccagatcgag ttgtaccagc cgctcactgt ctcgttcaac ctgtcgaagt
     9301 catatcccaa tccctatgac cccgacaccg tcgacgcccg cttcgagttc accggtccgt
     9361 cgggcgtcac ccgggtcatc ccggcctact ggaccgaaca gactccgcga tggcgggccc
     9421 gaatcgtgcc tttggaaacg ggcccgcact cgtgtcgtat cgtggtcaac gactctgcag
     9481 gtgattcggg cagcgccacg ctctccttca tgggtgtgtc ttcctcaaga cgaggcttcg
     9541 tccgcatcga cccccgcaac ccccgctacc tgcggttcga taacggcgag ccctacctgc
     9601 caatcggaca caatgtctgc tggtacggct gggatcccct gttctcccaa tggcttgacc
     9661 ggatgagcca ggtcggcgag aactggacgc gatactggat ggtcgcctac gtgtcacagg
     9721 acatcgaatg gggcggtggc accagtttcc agctcggccg ctattcgcag tccaacgccg
     9781 ccctgatcga cggcatgctg gaggccgccc gtgcccgcgg catctatgtc cagttatgcc
     9841 tcgattcgtt caacggctgg aacgaaacgc tctatgccaa ctgggccgac aatccctaca
     9901 acgccgccaa cggggggatg tgcacgcgcc cgaacgacta cttcgtcaac gccgaggcca
     9961 aacgcctggc caagcgccgt ttccggtaca tcgtcgcccg atgggcgtgg aaccctgcga
    10021 tactctgctg ggagttcttc aacgaggtgg atgccgtcgg cggcggagga gcaaccttct
    10081 ggggccatga agccgaaatc gcccagtggc atcaggaaat ggcccagtac atccgctcca
    10141 tcgatccgtt cgagcacctg cgatcaacca gcttcgccga cgacggccca cgttcgagct
    10201 acgcgccctt ctggcaactg cccgagatgg agatcgttca ggttcatcaa tacagcaccg
    10261 ttctacctca gtcgcatgtc aacctcatcc gccaggtccg ccagttcggc aagccggtca
    10321 tcatgggcga gggcgactac gccggcggcc cagacagcct tgaccccaac gggcagagcc
    10381 tccacgacat gatctgggcg gcggcagtcg ttgaaagcgg agccatgtcg tggtggtggg
    10441 acaactggat tcatcccaac aacctgtatt accgatttga gccgctggtt gccttcctca
    10501 gcagccagga ctgggcgccg cagcagctct cgccgctcgc ctatacgatc ctcagcggcc
    10561 acacaacgcc cgagatctac ggttcgggcg ggtcgaggca tggctacctc tacatccgca
    10621 attggcaagg cccggtcagc ggccttcgcc ttcgtctgaa tcagatcaca tcgcccggca
    10681 actacgtggc cgaatactgg gatacgaaca gcacgacgcc tttccgctcg gagattatcc
    10741 ccggcagccc aagcggcctc gaactcaaca tacccgactt cgcccgcgac gttgccgcca
    10801 agcttaagca aacgggcccg gtgttgagcg cagtccccac cttgctcacg gtcagcagct
    10861 tcatcggcca cgatgccagg ccgcagtcgt tcgccatcgg caataccgga acgggaagca
    10921 cgaactatga gatcacggtc gaccaattct ggctctcggt ttcgccgtca tcgggcggca
    10981 tcgaacagga aaccgacatc atcgacgttg cgtatcagac ggccgggctt cccctggggc
    11041 tctacacggc ctcgatcacg gtgtccgata ccggtggtca cagcccgccg gcagtggtca
    11101 gcgtgacctt gcaggtaagg ccgctcccgg cggatctgga caccgacgga gacgtagacc
    11161 aggaagattt cggccgactg caggtgtgct tgtccggcaa cggcgtcgtt caaacggccc
    11221 ccgagtgcga tctcggccgg ctcgacaacg acggcgatgt agacagggat gacgtcacgc
    11281 tgtttctggg atgtatcagc ggaccgtcgg tcccagccga tcccgagtgc gtcaaatgag
    11341 agcatatgtc aaaaccgcag cgtcgaggtt gccgcggcga ccgagacctg ccatattgtt
    11401 gagctcgggc cggcaggtct ccgctgcctt cggcggctcc gaccatgccc tgccctcttg
    11461 acacgcgttc tgagtgacaa ctgcgtcgga gccgttcagg acgagggtga ccgtccgtat
    11521 cgacgtgctg tacgctctcc actcccgcgg agaaaggagt tgaaacggga tcccgatgtg
    11581 agacggttct ggtcggtgtc cgccatcagc gacttgcttt cgcaccgaca ccaagccatg
    11641 acacattgct gaggcttcac attcaaaggg cggaaatcat gtcatacatc cgaatactgc
    11701 tgctcggtgg cctcgcgctc tggacgggtt gttcagcacc gacaaccatc ggctatccgg
    11761 ccgtctcgcc cgatggcaaa ggtttcgtta gcggcggaac gtcctttctc gtctggggct
    11821 tcaactacga ccgctcggtc atctcgggac gggatgtgtt gctcgaagat gtccttcggg
    11881 aacagccggc caagctcgat cgcgatttcg cgatgatgcg aagaatgggc ggcaacaccg
    11941 tgcgtctctt ctgccagatg ggtgaatact ttgagcgtcc cgaccacatc aatgctgccg
    12001 cgtttgaacg gctttccatg gcactggacg cggccgaccg ggcagatctg aaggtcctgc
    12061 tcgtggggct gtgccacata cgcaagtcga ccatcccggt gtggctcgtt gaggcggatg
    12121 acgacacggt cgaaagacac gaggagctgt tctggcgcca aacggcgaaa cgattccgcg
    12181 accatcctgc catcctggcg tatgacttgc aaaacgaacc ggccgtccac ggcagcgaca
    12241 gccgcgacct ggtcgtcggc tgcttctcga tgcgcggcgg cgagcagttc tgctacgtcc
    12301 acatgcacgg gcggcaggca tccaggcggt gggccgagtg ggtgcacggt cggttcggaa
    12361 ccgaagcggc agtgcgggcg gcgtggaaag actatcctcg tccgggcgag aactggttct
    12421 cgccggccct tccgaagacc tcccgggagg acccccgttt gggagacctc ttggcctgcc
    12481 accgggacct tctgcaggcc tgggctcaac ggatgcggtc ggccatccgt gagaacgacg
    12541 gcaaccacct gatcactgtt ggtgccctca accccgccgt cttcgccgac atcgtggatt
    12601 tccattgtgc ccatctttac cccaaacagg acctcacact cgaagccaac gaacaggctt
    12661 ggcgcgatca cttgtcgcgg cttccgcccg gcaagccgat cgttgtcgag gagttctacc
    12721 ccctcgccta cccccatcgc gccgacggcc aagccgcaac ggctacagaa ctgatcgccg
    12781 ccgtccgccg agccgccggg ccacacgggg cgggcttcgt gagtttctac tggggaccgg
    12841 ccgaggaggt catcgaaagc cccatgggac aagctctcta tcagaactgg ctcgacgcgt
    12901 ggtcatccgc gggacgcgtc gcctctggga ccccgtcgac cacccactcg cggtaggaca
    12961 ggaccgctga aggcggcgca gacccgccgc caccggttaa ccggcacgca gcaggcctcg
    13021 gttgccgtgg caccccccca catgccgccc cgacggtagg cctgcggtgc ttttcgcggg
    13081 ggctcttacc ggtctgtgga atgggcctgc ttgtctcctg aaccgaattc cagcttgatg
    13141 cccacgcgga cagcgccggg taatctggtg tggtaaattg ccctgcccga cagccggaag
    13201 atgcgcccgc tgacgtgcag ctcgaccgta acagaggtat tcggctcaat cttgcgcggc
    13261 acgcggagag ccatgccttc ttcggaaacg tccagcaact ggcacccttg aagggtttgt
    13321 gctgcctgct tgtcggcgga gtcgatgaag accgtcgccc agccggccat tcggaaacgc
    13381 agatgttttc tacgtccctt cgcataggac ggcgacgaat ctcgctcttt gtacgtgaag
    13441 agagcctcgc gccacttttc ccttgtcatg tactcagcga caagccctgt cgacatgagt
    13501 gtcccccgtt ctcggctgtc gtttgtccgg ccagttgtct attcggcgat tatcgcatgc
    13561 ctctgcaaca ggacggcgac atccttccta ttctccccgg ttatccggac ttatgcggaa
    13621 gctgtttccg aaaaacactc ccgccgagta agggctgttt gcccgatgcc gccggccgaa
    13681 acggtagatg cccggagggt tcggcccggg tcggcgacga actcttgatg ccccgggcgg
    13741 cgcctttgtg ccaggcgtct gcctgtctgt tgccgtttgc ccttgaatcc aacgcttgtc
    13801 cttgcccgtg cggcggcgaa ggtctccctt ggcccatcct tgagctgccg ccagtgtcct
    13861 acggtggtcc ggcgatattg cattcgcctt gcgttgttcg taccttgata ggggtggcac
    13921 gctcttcgtg cccggcggcg agcgaagtcg caagacacgc ctcccgggcc cgtagctcag
    13981 tggtgagagc aggtgactca taatcacttg gtcgggggtt cgaatccctc cgggcccata
    14041 agcttacagg catcggactc acactcggtc ttaccgcatc ccgaggcgtc gcgatcgcgt
    14101 tcgtcatcgg ccggttctca cgtgccgcct tcccgaggcc tccggaacgc gcaccgcgaa
    14161 ccccactact cgcccatgct cacgctcggg ctcgccggct cccgggacca tcctctctcg
    14221 gaaaacccgg gcaggaacgg tttcgccgat gccgctctcg gaacggactc cgccgcgcag
    14281 gacgtggtca aggctgatga ttccgcggcc agggaaatcg agccagaacg cgcaccccgc
    14341 cgcggcgcag gctctccacg cgcagctcac cgcccagggc cgtggcccgt tccctcatgc
    14401 ccacgattcc aagggacgcc gtcccctcaa gtttcgacgg atccgcaccg gtgccattat
    14461 cggtcacacg cagttcaata cccctgggcg tcgcccgaag cttcacttcg catcgcgtcg
    14521 cgtttgaatg ccgaagaacg ttggtgaggg cttcctgggt gatccgataa atcgtcgttt
    14581 tcaacgggtt ctgcacgttc agattgggcg gctctgcctt gacgcggcag gtcaaaccgc
    14641 cgctctggcc gtactcggtg atcagagact gcaatgcctc cggcaaggtc tggtagtcaa
    14701 ataccgcggg acgcaggctt ttggccaggt tccgaaccgt ctccgtcatg cgcgtctgca
    14761 agtctctgac ttctgaaagc cgctctctga tcggcggctc caccgccgcc aggtgagact
    14821 ccagccacgc caaggcaaga ttcatggccg tcaactgctg gccgagttcg tcatgcagct
    14881 ctctggagag tcttgcccgc tctttgtcga gcagggccgc agcctcgcgg cccaaccgcc
    14941 ggatctgttc gagttgagcc tcctcgcggc tcatgtccgt gaagtttccc acaaccagct
    15001 caccgccgtc gtccaggacg agccggctga acgtcgccgc gagagacacg accgagccgt
    15061 ccttgcgttc ggtgcgcaga cgcccacgcc agacccccgt cttgtgcaaa ccggccaaca
    15121 cgttgtcgcc gtgcccttcc ccctttcgga tactgtccct gaacgctttc aagacctcca
    15181 gctcctcgag ggacgagcaa cccagcatgg cgagcatcga ggcgttggca taggtcaagt
    15241 gccagctccc gtcccagatg gcgatgccct cccctgcgtt cttcaccgcc tcggccaacc
    15301 gcacggaagc ggattgcatg gctctccatg cggtgatgtc acgcatcagg acaatgcagc
    15361 ccatgggtgc gcccgactca ttgcggagca gggtgctgcg catcgacgcg aggaaggttg
    15421 aaccgtcctt ccgtacatgc cccgtttcct cttcgaactc gcctttggtg acagtctgcc
    15481 ggattgcctg atagacggcc ggcgcctgtt cggaagtgtg aaaaaccgac aaaggcttgc
    15541 cgagaaagtc ggcgtctcct ccgccgtgca tcgccgcgca cgcccggttc aggaacaaca
    15601 ccttgccgtc cagatcgacg acggccacgc cttcgctggt ttgctcgacc acttgcgaaa
    15661 gcagcttcag ccgttgctgc gcttcacgtt ccgccgtcac gtcgataatg atggaatcat
    15721 gtctcacgag ccggttgttt gagtcatgca cgggcacgac gtaccgttgc aggtaaacag
    15781 gcgttcgacg tttgccgtga atgatgcggt atgtcatgct gaacggcttg cgcgatgaga
    15841 tgccttcttc gaaactcgcg tatgtcgccc gccggtcttc cgggtgaaca atggctgctc
    15901 ccaggtgcgg gtccgaaaag atctgcgcat ggtcgtagcc caggatacct tccgtgtcgc
    15961 cttccagcag caggacacgc cccgtgtccg cctcgaccga ggacagggcg atcggaagcg
    16021 actccaccat ctgtcgtatt ctggcctcgc tccgttgaaa cgactcctca acccgccgac
    16081 gctccgtgat gtcacgcagg atcgtggcca cgcgaaccac ctgatcagat tccatgatcg
    16141 ggattttgcg gatctccaat acgacggacg tatcctgcac gcgcgtctcg agctcgtatt
    16201 cgatactctc gccgctgtta aagatctgtt tatagtggtc aaggctgctc tcgggaataa
    16261 ggggaaccgc ctcggttaat ggccggccca ccggttccgc ccggatcccc aagcgctcaa
    16321 tccagttgcg aaaagcctga ttgaccagca cgatccggta atcccggtcg atcacgtgaa
    16381 tcgcgtccgt catggcgtcg agaacggcgc gatgctggcg ctcagcggcc agcatttcct
    16441 gctccatctg cttgaggtga gtgatatcgc gattcagtcc gacaaacccc acgacttctc
    16501 ccgtacgttc atcccgcagc ggaaccttcg tcaccagggt ccacaccggg gccttgcccg
    16561 gataccgggc ctcttgttcg acgttgacca ggggctcgcc cgtttcgaga atgtgacgct
    16621 cctcctgcaa atactgctga gccgcatccg gcgggaagaa atcagcgtcc gttttcccga
    16681 tcaactcgct caggtttgaa agcccgaatc gcgacagaac ggtccggttc accagacgga
    16741 agcgcgactc gcgatccttg acgtagatct ggtccggcag atgatcgatc agcgtctgca
    16801 gcaggttccg ctggcgatcc agggcctgcc gggtgaggtc ttgctcctgg gcgcgttgct
    16861 ccaattgttc gcttgcccgc aacagttcgt ccccctgcaa acgaaaacgc tcctgaagca
    16921 gttggtgcgc cttcttcagt tccgcctcgg cttgtttgcg ccgggtgatg tcagtgaacg
    16981 tacaacccac catttgcgtg aggccttcgt ggtagatggg gttgtaccag atcagcgcat
    17041 caaactcgtg cccgtccctg cttcgcaggc ggcattcgat ctcgcctccg ccccgaccga
    17101 ggcccc
//
LOCUS       DAOTPW010000118        17100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3337_length_17100_cov_5.661954, whole genome shotgun sequence.
ACCESSION   DAOTPW010000118 DAOTPW010000000
VERSION     DAOTPW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17100
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3337_length_17100_cov_5.661954"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="P5316_11920"
     CDS             <1..1243
                     /locus_tag="P5316_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85705.1"
                     /translation="QQQYATALVPGADLAEGRTRVLTFSFADASSRSKAIESLQRELL
                     QGEGNEEAKSSAGVPHAPRRSVAAPQETHPAEMAPQPSAQAGDSVRHRRQELGVRSLR
                     DGGTNGVVADDWGAAPGAVKVASIDTNPDSAVNVLKVVTVNGASADRLMAVLNNRDWS
                     AGRHRYLAEDAEAVQLRDHKAKEDGPGPDRRPTTTVFDDAADRVWDDDDGDGVSDWMK
                     LPTALPKPPATQPSRKSLYLSDADTISPLDGKMTWDLARIFENGGRDDVMTDEEGVAN
                     QVYDMALRPDRSIVLDTGAEPHATTSPADSPQDEASYGTGGMGMMGGAVAGTGLEGMG
                     AGYGGYAAAQSKGSTPRYAGSWFGQSSSQPASAGGSRPADSNLLVVYFEVLSPDTQPT
                     AASSTSAPASADLTSQPAANP"
     gene            complement(1220..2161)
                     /locus_tag="P5316_11925"
     CDS             complement(1220..2161)
                     /locus_tag="P5316_11925"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRR85706.1"
                     /translation="MRILTLAVVTLTMAGCQHGGLGQGTSGTLEVRAMSFNIRYGTAN
                     DGPNHWDKRREMVFQVFRDHRPDVVGLQEALRFQLDEMREALPEYDEVSAGRDDGKAG
                     GEASSIFYRSARFDVQEHGTFWLSDTPEVPGSRSWGNNITRICTWAHLHDRQSGATFY
                     MFNTHLDHQSQPARELGAELIAQRMGGRAHPDPLILTGDFNAGEGNPVVRYLKGEIAT
                     VPAKRGPCAVVPALVDTFRVLHPDVKIVGTFNGFSGNREGEKIDYILASPEWTVAEAA
                     IVYDNTDGRYPSDHFPVTAVLRLSPPSGTGSRVRGGL"
     gene            2409..3224
                     /gene="lpxA"
                     /locus_tag="P5316_11930"
     CDS             2409..3224
                     /gene="lpxA"
                     /locus_tag="P5316_11930"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRR85707.1"
                     /translation="MPIHPSAIIDRRAEIDPTADIGAYVIVEGVVRIGAGTRVYPHAY
                     LTGWTEIGPNCEIHPNAVIGHAPQDRAYGGEETYCRIGEGTIVREGASIHRGTAPGSA
                     TVVGDRCFLMANSHVGHNCQVGDDVKMANGALLAGHVHVGDGAFLSGNTAIHQFCRVG
                     DLVMVRGGIAVSMDVPPYFTVGLSGLCAGINIVGLRRAGFAPEQRREVQQAFRTLYRS
                     GMTFRKALDELAQTVKTDAGRKIVEFLRSPSKRGIVGCIHNRRGRATAAETEE"
     gene            complement(3272..5215)
                     /locus_tag="P5316_11935"
     CDS             complement(3272..5215)
                     /locus_tag="P5316_11935"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR85708.1"
                     /translation="MASTTTAAYRSRQPDADAGALVNRILLPLCLGCAAAIVGSCLPA
                     AVAAGGLVGIADKAGVRAQTLAICLFMFPFFLLGPFGGLLADRLPRRGLMIAVGLVAT
                     AVTAGLVVLTSRRPLAPSWNVLILTGGGIALALLWPAKSALLPGLVSRHRLARANAAS
                     CALVVLAVAVAVLAGGYLADRQLPRTALLASSVSFLFSSLLLCLIRQPAGKDRRPPRH
                     VTGIGTLRETARYICGHRRAARLILLNMLVWGCGALIWGAAPTLMRDVWGRQGVIDVR
                     VFQSFLAGGLLVGALILTSIADALRGEAIIAWSLPAVTLAIAVLAGLALLPMSSAPAF
                     WAGAAAAAFAGTFFAGATVACNALLQRIVPNRLRGGVFGMSYLTGTAALLAVVGALGI
                     PRWSNLSDWIGWVLLGAAAVVTGISARVWSIRLRHGPLPPAKQFWWNLNEFYCKWWFR
                     FRREGACTVPAEGAVIVVANHTCGIDPLLLIAGTSHRLIGFLVAEEYTHIPIGGRLIR
                     MIGCVPVRRDGHDAAGTRAALRHLRDGRALGIFIEGGIPEPGEVREPKLGAAMLALHS
                     GALIVPAHIWGTHYDENIARSFFRRHHARVRFGKPIDLSRYWIPSGDKEALRRVSLRL
                     LERIRQLGPGGEMEDDAPINVSP"
     gene            5527..7233
                     /locus_tag="P5316_11940"
     CDS             5527..7233
                     /locus_tag="P5316_11940"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRR85709.1"
                     /translation="MRTLQLYVTRELLKTFLLAAVGLTLVLSLCGTMSTVLRVEVPTA
                     TTVLYILWLIQPMVLAVTLPVSALFACAIVYGRLAADNEFDACRASGINIHRLLAPAL
                     GLSIFTALFAFAFSNYVIPKAAAGIASRVQYDISKFAFQALKTRGYFKREDKKKSEAQ
                     ILHAGKVDLVQEGGHDVLLIHDGAFIELKGDELVRCGTVRQGRADFKTAPESGEPLVE
                     AFLTDVRLVDLQDIQQARLYQEKTRPFGSRELPKVLFYDPKWSSLGDLLKYKNDPSQL
                     PPLRPQLGEIRREVRESLFYRRLCGHFAASSGPLIIEDETQRYEIRAAGARMIEENFT
                     PELRDVSVKCAWTEEKGRKRTRVYQAESATVRLQRNIANTRDIVLISLLGGVTFTDSA
                     QPGRTFPHAALDLQPIAAPPSLLEDDQQYSDARLLGLGDIMNRKIRSRAQLQSAVKPL
                     GLGPQIEKLRLKAVLDMLRQRHQIIGSLHSRLGLCASALVTIILAAALGIIFRNGQFL
                     TAFAISFLPALAIVAMNMLGRRQAESIDPHLHVLGVGIIWAGILLLALVDLVVLKRYL
                     RR"
     gene            7247..8410
                     /locus_tag="P5316_11945"
     CDS             7247..8410
                     /locus_tag="P5316_11945"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRR85710.1"
                     /translation="MVRILDRYLLRNFLANYILSLLMLISLYVVLDLSFNADEFTETM
                     TEGPQQQSSFLGGAIKVLANIIDYYVFNIPLYFAQLSGVITLFAACVTLGRLQRQNEM
                     TAVLSSGTSMYRLAMPIILAGLAMNALLILDQEMLIPALAPKIARPRDDVEGARIHKD
                     LWYIKDADGRLFSAQEFLPKEQEIGGLIVIERFTDPGDPANYGQLRSVIRADHARWND
                     RNGGWELTQRAFRLDIGRRAEFIVSDTGERLTPVVTDFYKTDLSPANLLLRKSAQWLD
                     FLSLRQIKTLQSRGDADPVRVARIRHTRFTLPISNMVLLLLGMPFFMNRLPGNVLGQG
                     SKALAICAVAFLTTFLGQQVVGTTGGFWQALPYWLPIFIFGPIAVLLLDNVDT"
     gene            8482..9243
                     /locus_tag="P5316_11950"
     CDS             8482..9243
                     /locus_tag="P5316_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRR85711.1"
                     /translation="MSKRVLAFMAHPDDVEFTCAGTLIRLKNEAGCEIAIATATSGDC
                     GSVRHRPDEIARIRHAEAVAAAGLLGADYYCAGCSDVFILYDEPTIRRFVELIRKTRP
                     DIVITQPPTDYMVDHEMCGKLVRTATFIAGAPNLLTRDIDPAPPTERVPHLYYADPTE
                     HIDPIDGRPVTPDLVVDITSVMPTKEKMLACHASQRDWLRKHHGMDEYIEIMKRGGAA
                     RGKLIGKPYAEGFRIHRGHPYPQDDVIAELLKIRK"
     gene            complement(9319..10398)
                     /locus_tag="P5316_11955"
     CDS             complement(9319..10398)
                     /locus_tag="P5316_11955"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HRR85712.1"
                     /translation="MAKKTTAAGKYADVPVMNVLQLGGIETSVLDNGPGRDVRIAWVN
                     TGGGLRYKVVIDRGLDIADAEYMNQSLTWLSLTGTNAPSHAYNRGMDWLRGFYGGLVV
                     SCGPLNTGAPFSEEGHDYGLHGTHSHSRAIIESIVNPDLPTGKLDMSITGLVRTARVF
                     GPNVELRRTISSTLGEPTIRIRDRFVNCGNQPVPHCWLLHINFGYPLLEPEASTYCYS
                     GKLTCRGDSAEWFGKRKDFRAAPKPQETHRGTGEVFTYIDPKTDARGMVLAGVVNRKR
                     GFGVKIEFPKRQYPRMGNWQHWGPCGSYTGALEPMTAGVEGRPIDRDRGWLRMLEPGE
                     TVDLTCNITATSDKAELDRLLRLNK"
     gene            complement(10598..12166)
                     /locus_tag="P5316_11960"
     CDS             complement(10598..12166)
                     /locus_tag="P5316_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85713.1"
                     /translation="MTVRVQTDSTPETATTDVPSMHSVADTGPRGSLLATVAPWLRPA
                     KWAPVVARMPLWSCFTVHLLGALAAFVTCMLMIDLESTASGDSWSPWADFGLFWMDHP
                     DIAAVVTGITAGFIELGFLAAAFILTPWGAGDEPLRCSYAHALRHTWLLSTQAVPLIL
                     LIGLMIVPISAAARSFEARHPYPSFTFPTPPTYPAGAAPDSQAMKDYQAAQAEYTLQI
                     SSYNKRWLQTLREYESTRPFIVRNSAEIIGWTCIAGVVWVLWSLLRAIGAPRKLPSIV
                     RPPTCEFCGYNLTGSALDGRCPECGTPAIDSLGPDVRPGTPLDRSTKEHWFAAWWKSS
                     IEAMRRPCQFGRLIQINQPSRRYRWNLAAPLLVGILAASAAFCASLWIHFRNSDDDFS
                     LIVGKEFFTVAAPLAGISSAVSVVGATLLIAAIVGWILSWQNNRNLLPAAMQMQAHLG
                     LLTILWLLFGCGWTILTFYVESMDVFLAMQRVLLIHNDPLEFFFWCMPVAAWLLLDVV
                     LVWKGTVAARYANR"
     gene            complement(12217..13380)
                     /locus_tag="P5316_11965"
     CDS             complement(12217..13380)
                     /locus_tag="P5316_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85714.1"
                     /translation="MTDFSARLHGSPVSTVGTVRNSFQNDPVGVVVGAFMLLIAVEAG
                     CLFLAWLLTPWGAVDELPAASFVHALRLVWLHTTHAVPLIFVSGTAMTEYNYLLRFGD
                     PVAHDYAQQYGGYVVSCVIAACSAWFLWALFRATGASRAATPSSRPPTCDYCGYNLTG
                     ARPDGQCPECGVPVPMSLGSEARPGTACDLTRPTAFGAAMRRCWRDAVFRPAGLGRQI
                     RVTQTSRNHSRCLTAILLPLWCVSAVAFALSVMAPETALNRVVVARTLLAAPVFGAAS
                     AAAVLIMALLIAAVVGGVLSRCHGRNLHPAAMQMQCYLGGFWLVWAVLVWVWLVCCTL
                     LAMEGAFRSMGDTLSIDGALLGFLCWFLPCAAGVVVNFVLVVKGTAAARYANR"
     gene            complement(14210..14392)
                     /locus_tag="P5316_11970"
     CDS             complement(14210..14392)
                     /locus_tag="P5316_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85715.1"
                     /translation="MALTPEERDWLLEQKRELKRELDEIAAAKAWPGYVDKNERRIEL
                     LDALGKVERQLREDRD"
     gene            14552..14839
                     /locus_tag="P5316_11975"
     CDS             14552..14839
                     /locus_tag="P5316_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85716.1"
                     /translation="MTLLHTFLPLVSTCSRSKALTETAFLTLSEGIALTRIVTGRDAA
                     MISSETTGEKSTNGGHRRAGRAASVKPTDADMAPLYQEVEKAIPWMPGERQ"
     gene            14998..16050
                     /locus_tag="P5316_11980"
     CDS             14998..16050
                     /locus_tag="P5316_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85717.1"
                     /translation="MLHALALSAVLTVSAVPDEFIVLSEFQVWADPAGSEVFKELNRR
                     YLKTGRSHVFNLYPRHDYFVWELPKTRGWVDEAIILGAFNVFCIGDDIRTAEGFLFDE
                     KGLSPRFKDVLFGTIAYAHEKGLMVAVEPQGLPTTKDDEHLQRWFRSWLGPDIPRDQQ
                     ADILKLSLDWFGANQFNPQLAEEIEAVFTACRAVNPDVLIYLDSVHGIWRTPTPFHRW
                     LLQRFPQTIISHYLNTDQIDAFRKAGAGNMMVQINPSEIEEVGGQFFIFHDKTVAWLQ
                     DAVRRRVRYLSLAGVNYGYNRYNYDLFLDVIRPHLALKRNLQAVRESLLPDRIAQPTT
                     KEDVRAWLLEQSNKGK"
BASE COUNT         3365 a         5352 c         5160 g         3223 t
ORIGIN      
        1 acaacagcag tacgccacgg ccctggttcc gggggcggat ctcgcggaag gtcggactcg
       61 cgtgctgacc ttctcttttg ccgacgcgag cagccgcagc aaggcgatcg agtcgcttca
      121 gcgagagctt ctgcagggtg agggtaacga agaggccaag tcgtctgctg gtgtgccgca
      181 tgcgccgcga cgcagcgtag ccgcaccgca ggaaacgcac ccggcggaga tggcccccca
      241 accttctgct caggctggcg attcggttcg ccaccgcaga caggaactgg gagttcgttc
      301 ccttcgagat ggcgggacca atggggttgt cgcagatgat tggggggcag ccccgggtgc
      361 ggtcaaggtc gcctcgatcg acacgaatcc ggattctgcc gtgaacgttc tcaaggtggt
      421 caccgtcaac ggcgcctcag cggatcgact catggccgtg ctcaacaacc gcgactggtc
      481 cgccggccgc caccgctatc tcgccgagga cgcggaagcg gtgcaactcc gggaccacaa
      541 agcaaaagag gacggcccag gaccggatcg acggccgaca acaacggtgt ttgacgatgc
      601 tgccgacaga gtgtgggacg acgacgacgg cgacggagtg tcagactgga tgaagcttcc
      661 aacggctctt ccgaaaccac cggcaacgca gccgtctcgg aaatcacttt acctctctga
      721 tgccgacaca atctcaccgt tggatggaaa gatgacttgg gacttagctc gcatctttga
      781 gaacggcggc cgagacgacg tcatgacgga tgaggaaggc gtggccaacc aggtctacga
      841 tatggctctg aggcctgacc ggagcattgt ccttgatacg ggcgcggaac cccacgccac
      901 gacttcacct gccgattcgc cgcaagatga ggcatcttac ggcacgggtg ggatgggcat
      961 gatgggcggc gcggttgccg ggactggtct ggagggcatg ggcgcaggct atgggggtta
     1021 cgccgcagca caaagtaaag gatcgactcc caggtatgcc ggctcttggt ttggccaaag
     1081 cagttcccaa cccgcgtcgg cgggcgggtc ccggccggcc gatagcaacc tgctggtggt
     1141 gtactttgag gtgctctcac cggacacgca gcctacggcc gcctcgtcca cctccgcgcc
     1201 ggcctctgca gatctgacgt cacagcccgc cgcgaacccg tgatcctgtg ccggaaggcg
     1261 gggataaccg caacaccgct gtgaccggga aatgatcgga tgggtaacga ccgtccgtgt
     1321 tgtcgtacac aatcgcggct tcagcgaccg tccattccgg cgaggcaagg atatagtcaa
     1381 tcttctctcc ttcgcggttg ccggaaaagc cgttgaaggt gccgacgatc ttcacatcgg
     1441 gatgaagcac gcggaaggtg tcaaccaggg ccggcacgac cgcgcaagga ccccgtttgg
     1501 cgggtaccgt ggcaatctca cctttcagat atcgcactac cgggttgcct tcgcccgcgt
     1561 tgaaatcgcc ggtcaggatg agcgggtccg gatgcgcccg gccgcccatt cgctgggcga
     1621 tcaactcggc gcccagttcg cgagccggtt gagactgatg gtccaagtgg gtgttgaaca
     1681 tgtagaaggt ggcaccactt tggcggtcgt ggagatgtgc ccaggtgcag attcgggtaa
     1741 tgttgtttcc ccaacttcgc gagccgggta cttcgggagt gtctgacagc cagaaggtgc
     1801 cgtgctcttg cacgtcgaag cgggccgagc ggtagaagat ggacgacgcc tcgccgcctg
     1861 ccttgccgtc gtcgcgtccg gcagacacct cgtcgtattc aggcagcgcc tcccgcattt
     1921 cgtccaactg gaaccgcagg gcttcttgca ggccgaccac gtccggccga tggtcgcgga
     1981 agacctggaa aaccatctcg cggcgtttgt cccagtgatt gggcccatcg ttggcggtgc
     2041 catagcgtat gttgaaactc atcgctcgca cttccagtgt gccgctcgtg ccttggccga
     2101 ggcctccgtg ctgacaacct gccattgtca gtgtcacaac cgccagtgtc agaattcgca
     2161 tggattctcc tctcgtgaac aaggtccgca tgtcggccgc ccggatcgtt cgaaccaccg
     2221 atggtaagcg ttcccgccgg tctcgccaag ctgcgccggc ggctggcctt ctgcggcagt
     2281 ccgtgtatag tgttcacgcg tttgcccggg tggcggtgcg gtggccggtc cgggccggtt
     2341 tgccgcgcag gatttgtgca tcacgccacg gcgggcagta tccccggatc acgaggcggc
     2401 agcacggaat gcccattcat ccttctgcaa tcattgaccg gcgagccgag atcgatccga
     2461 ccgccgatat cggtgcctat gtgatcgttg aaggcgtcgt tcgcatcggt gccgggaccc
     2521 gtgtctatcc tcatgcttat ctcacgggtt ggaccgagat cggtccgaac tgcgagatcc
     2581 acccgaacgc cgtcatcggc cacgctccgc aggatcgggc ctatggtggt gaagagacct
     2641 actgccggat cggtgagggc actatcgtac gagaaggggc gtctatccat cgcgggacgg
     2701 cccctggttc ggcaacggtg gtcggcgatc gctgtttcct catggccaac tcgcacgtcg
     2761 ggcacaactg tcaggtgggc gacgacgtca agatggccaa cggggcgctc ctggccggcc
     2821 atgtccacgt tggcgacggc gcatttctca gcggcaatac cgcgattcac cagttctgtc
     2881 gtgttggcga cctcgtcatg gttcggggcg gcattgccgt aagcatggac gtgccgcctt
     2941 attttaccgt cggactgtca gggctatgtg ccggcattaa catcgtcggt ttgcggcgag
     3001 ccggttttgc ccctgagcag cggcgggaag tgcagcaggc cttccggacc ctttaccgtt
     3061 ccggaatgac ctttcgaaag gccttggacg aactggccca aaccgtcaag accgatgccg
     3121 gtcgcaaaat cgtcgagttt ctccggtccc catccaagcg cggcatcgtg ggctgtatcc
     3181 acaaccgacg cggacgggca acggctgccg aaaccgagga atgagcggct gccgcaagcg
     3241 tcattcatcc tgaatcagca tcccgcgcgc atcacggcga tacattgatc ggcgcgtcgt
     3301 cttccatctc gccgcccgga cccagttgcc gaatacgctc cagcagtctc aagctgactc
     3361 tcctcagcgc ttctttatcg ccgctgggga tccagtagcg gctcaggtcg atcggcttgc
     3421 cgaatcgaac gcgggcatga tggcgacgga agaaggaccg ggcaatgttc tcgtcgtagt
     3481 gtgtgcccca gatgtgcgca ggcacaatca gggcgccgct gtgcagggca agcatggcgg
     3541 cgccgagttt gggctcgcga acctccccag gctcagggat gccaccctca atgaaaatcc
     3601 cgagtgcacg gccgtcgcgc aggtggcgca gagctgcgcg tgtgccggca gcgtcatgcc
     3661 cgtctcgccg caccggcaca caaccgatca tgcggataag ccgcccgcct atcggaatgt
     3721 gcgtgtattc ctctgcaacc agaaacccga tcaaccggtg cgatgtgccc gcgatgagca
     3781 acagcggatc gatgccgcag gtgtggttgg ccacaacgat gaccgctccc tcggccggca
     3841 ccgtgcaggc accctcccgg cgaaaccgga accaccattt gcagtagaac tcattcaggt
     3901 tccaccagaa ctgcttggcc ggtggcaacg gtccgtgtcg cagtcgaata ctccagactc
     3961 tcgccgagat cccggtcacg actgcggccg cgcccagcaa gacccagccg atccaatcgc
     4021 tgagattcga ccagcgcgga ataccaagcg ctcccaccac cgccagcaac gcggcggtgc
     4081 cggtcagata cgacatcccg aaaactcccc ctcgtaacct gttcggcacg attcgctgga
     4141 gcagggcgtt gcaggcgacc gtggcgccgg caaagaacgt cccggcaaag gcggctgcgg
     4201 cggctcccgc ccagaaggcc ggagcggatg acatcggcag cagcgcaaga cccgccagga
     4261 cggcaatggc cagcgtgacc gccggcagcg accaagcgat gatcgcctca ccgcgcaagg
     4321 cgtcggcgat gcttgtcaga atgagagccc cgaccagcaa accaccggcg agaaacgact
     4381 gaaacacccg aacgtcaatc acgccctgtc gaccccacac gtccctcatc agtgtgggag
     4441 cggcgcccca gatgagggct ccgcagcccc agacaagcat gttgagaaga atcagccggg
     4501 ccgcccgccg gtgcccacag atgtagcgtg cggtctcgcg aagggttccg atacccgtga
     4561 cgtggcgggg cggcctacga tcttttccgg ccggctgccg aatgaggcac aaaaggagcg
     4621 aactgaacaa gaatgacact gaacttgcga gcaacgccgt ccgcggcaat tgcctgtcgg
     4681 ccagatatcc acccgccagt actgccaccg caaccgccaa gacgaccaac gcacagctcg
     4741 cagcgttggc tcgggcaagt cggtgcctgg aaaccagacc gggcagtagc gctgattttg
     4801 ccggccagag caacgccaag gcaattcctc caccagtcaa gatcagcacg ttccatgacg
     4861 gggccagcgg tctacgagag gtcagcacaa ccagtccggc cgtcaccgcc gtcgcgacca
     4921 aaccgacggc gatcatcaga cctcgccgcg gaaggcgatc agccaggagc ccgccgaaag
     4981 gacccaagag gaaaaacggg aacataaaca ggcagatcgc gagcgtctgg gcgcgcacac
     5041 cagccttgtc cgcgatccca acaagcccac cggccgcgac cgctgcgggc aagcagctcc
     5101 cgacgatggc cgctgcgcat cccaagcaga gcggcagtaa gatccggttg acaagcgcgc
     5161 ccgcgtccgc atcaggctga cgactgcggt acgcagcggt tgttgtgctg gccatagctg
     5221 ttccttgggt acgaatcatg accttattaa tggcccggca ttttaacggt agcgggcgca
     5281 cgcttcccat gtgaggcggc gccgatccag agcttattcc atataccctt accggtaccc
     5341 cggcgcttcg cggatcgcgg gccaagccta ttgacgcccc gccagaattg ggtaggtttg
     5401 gtattcggcg cggcgagacg ttgcatgggc ttgttcttta gtccttgttt gacaaggcct
     5461 taggattcga tagcccattg gccgccagga gccggcccag accggcggca cccgaagggc
     5521 gctttgatga ggactctaca gctttacgtc acccgcgagc tgctcaagac ttttctgctt
     5581 gctgccgtgg gcctgacgct ggtgctgagc ctctgcggga ccatgtcgac tgtcctgagg
     5641 gtcgaagttc cgacggcgac gaccgtcctg tacattcttt ggctgatcca gccgatggtc
     5701 ttggcggtga cgctgccggt gtccgccctg tttgcttgcg cgatcgtcta tggccgtctg
     5761 gcggctgaca acgagtttga cgcctgccga gccagcggga ttaacatcca ccgtcttctg
     5821 gctccggccc tggggctcag cattttcacg gcgctcttcg cgtttgcatt cagcaactac
     5881 gtcatcccca aggccgccgc gggcatagcc agccgcgtcc aatatgacat ctccaagttc
     5941 gctttccagg ccctcaagac ccggggctac ttcaagcgcg aggataaaaa gaagagcgaa
     6001 gcgcaaatcc tgcacgccgg caaggttgac cttgttcagg agggcggtca cgacgtcctc
     6061 ttgattcacg atggcgcgtt tatcgagttg aagggggacg agctggtccg ctgcggcaca
     6121 gtcaggcagg gaagagcgga cttcaagacc gccccggaaa gcggcgagcc gctggtcgag
     6181 gcgttcctca ccgacgtccg gctggtggat ttgcaggaca tccagcaggc tcgtctgtat
     6241 caggagaaaa cccgaccgtt cggttccagg gagctgccca aggtgttgtt ttacgatccc
     6301 aagtggtcct cgctgggcga cttgctgaaa tacaagaatg atccgagcca gttgcccccc
     6361 cttcgccccc agctcggtga gattcgccgc gaggtgcgtg aaagcctctt ttacagacga
     6421 ctctgcggcc attttgcggc gagctcggga ccattgatca tcgaggatga gacgcaacgc
     6481 tacgagatcc gcgccgccgg cgctcggatg atcgaagaaa actttacgcc ggagctgagg
     6541 gacgtgtcgg tgaaatgcgc gtggaccgag gagaagggtc gaaaacgaac tcgggtttac
     6601 caggccgagt cggctacggt ccgcttgcag cgcaatatcg ccaacacgcg ggacatcgtt
     6661 ctgatcagct tgctgggtgg cgttacattc accgactcgg ctcaacccgg cagaacgttt
     6721 ccgcacgccg ccctcgattt gcagcccatc gccgccccgc cgagtctcct tgaagacgat
     6781 cagcaatact cggatgccag gctgctcggt ctgggcgata tcatgaaccg gaagatccgg
     6841 tcgcgcgctc aactgcaaag cgcagtgaag ccccttggcc tggggccgca gatcgagaag
     6901 ctccggctca aggctgtgct cgacatgctc cgccaacgac atcaaatcat cggctctctg
     6961 cattcgaggc tgggtctgtg tgccagcgcc ctggtgacca tcattctggc agcggccttg
     7021 ggcatcatct tccgaaacgg ccagttcctg accgcctttg cgatcagttt tctgccggcc
     7081 ctggcgatcg tggccatgaa tatgcttgga cggcgccagg ccgagagcat cgatccacac
     7141 ctgcacgtcc ttggcgtggg catcatctgg gcgggtatcc tgttattggc cctggttgac
     7201 ttggtggtcc tgaaaaggta tctgaggcgt tgaggcgagc cgggcaatgg tgaggattct
     7261 cgatcgatat cttcttcgca attttctcgc caactacatc ctttccctgc ttatgttaat
     7321 cagtctgtat gtggtgctcg atctgtcttt caacgccgat gagttcacgg aaacgatgac
     7381 cgaggggccg cagcagcagt cgtcattcct cggcggagcg atcaaggttc tcgccaacat
     7441 catcgactat tacgtcttca acatcccgct ctatttcgct cagctttccg gcgtgattac
     7501 cctgttcgca gcctgcgtga ccctggggcg acttcagcgg caaaacgaga tgacggcggt
     7561 attgtcctcg ggaacgagca tgtaccggct ggccatgccg atcattcttg caggtctggc
     7621 catgaacgcc ttgctcatcc tcgaccagga aatgctgatc cccgcgctcg cgcccaagat
     7681 cgcccgtccg cgcgacgatg tcgagggtgc tcgcatccac aaggatcttt ggtatatcaa
     7741 ggacgcggac ggccgtttat tcagcgctca ggagttcttg ccgaaggaac aggagatcgg
     7801 cggtctcatc gtcatcgagc ggttcaccga tccgggcgac cccgccaact acggccagct
     7861 ccgttcggtg atccgcgccg atcacgcccg gtggaacgac cgcaacggcg gatgggaact
     7921 gacgcaacgg gcgtttcggt tggacatcgg tcgccgggcg gaattcatcg tgagtgatac
     7981 gggcgagagg cttacgcccg tggtcactga tttctacaag acggacctgt cgccggccaa
     8041 tctgctgctt cgaaaaagcg ctcagtggct tgacttcctg agcctcagac agatcaagac
     8101 gttgcagagt cgcggtgacg ccgacccggt tcgggtcgct cgcatccggc acacccgatt
     8161 cacgctgccg atcagcaaca tggtgctgct cttgctgggc atgccgtttt tcatgaaccg
     8221 gctgcccggc aacgtgttgg gtcagggctc aaaggccttg gcgatctgcg cggttgcttt
     8281 tctgactacc ttcctcgggc agcaggtcgt cggtaccacc ggcggcttct ggcaggcgct
     8341 gccctattgg ctgccgatct tcatcttcgg tccgatcgcc gtccttttgc tcgacaatgt
     8401 cgatacctga gaaggcaccg ctgacggtca tggcgggggc gggttatgga aaagtgaagc
     8461 tgtttgtggg agtcccgtat gatgtcaaag cgagttctgg ctttcatggc ccatcccgat
     8521 gatgtggagt tcacctgcgc cggcacgctg atccggctca agaacgaggc cggctgcgag
     8581 atcgcgatcg ctacggccac gagcggcgat tgcggctcag tccggcatcg cccggatgaa
     8641 atcgcccgca ttcgccacgc cgaggccgtg gccgcggccg gcctcctcgg cgccgactac
     8701 tactgcgccg gctgctcaga cgtgttcatt ctctatgacg agccgaccat ccgccgcttc
     8761 gtcgaactga tccgcaagac ccgcccggac atcgtgatca cccagccgcc gacggattac
     8821 atggtcgatc atgagatgtg cggtaagctg gtgcggaccg ccacgttcat cgccggggcg
     8881 cccaacctgc tgaccaggga tatcgacccg gcaccgccca ccgaacgggt gccacatctc
     8941 tactatgccg acccgacgga acacatcgac ccgatcgacg gccggcccgt gactcccgat
     9001 ctcgttgtgg atatcacctc cgtcatgcca accaaggaaa agatgctcgc ctgtcatgcc
     9061 tcacaacgcg actggctgcg aaagcaccac ggcatggacg aatacatcga aatcatgaaa
     9121 cgtggcggcg ctgcccgcgg caaattgatc ggcaagccct atgccgaagg cttccgtatc
     9181 caccgcgggc atccctaccc gcaagacgat gtgattgccg agctgctgaa aatccggaag
     9241 taggcggggc cccgttcctt cgggttcgcg tcgcacccca ttccggcgac cgccacggaa
     9301 gcaagctctg ccgttttcct acttgttgag cctcaggagc ctgtccagtt ccgccttgtc
     9361 gctcgtggcg gtgatgttgc aggtcagatc gacggtctcc cccggctcaa gcatgcggag
     9421 ccagccgcga tcgcggtcga tggggcgccc ctcgacgccg gccgtcatcg gttcaagggc
     9481 cccggtgtat gagccgcacg gaccccaatg ctgccagttg cccattcgcg ggtactgcct
     9541 cttggggaat tcgatcttca cgccaaaacc gcgcttgcgg ttgaccaccc cggccaggac
     9601 catgcctctg gcatcggttt tcggatcgat ataggtgaac acctcgccgg tgccgcgatg
     9661 ggtttcctgc ggcttggggg cggcgcgaaa atccttgcgc ttgccgaacc actcggcgct
     9721 gtcgccgcga caggtcagct tgccactgta gcaataggtg ctcgcctcgg gctccagcag
     9781 gggataccca aagttgatgt gcagcagcca gcagtgcggc acaggttgat tgccgcagtt
     9841 aacgaagcgg tcgcggatgc gaatcgtcgg ttcgccgagc gtgctgctga tggtccggcg
     9901 aagctcgacg ttcggcccga agacgcgagc ggtacggacc aaaccggtaa tgctcatatc
     9961 gagcttgccg gttggcaggt cgggattgac gatcgactcg atgatcgccc ggctgtgcga
    10021 gtgcgtgcca tgcagaccgt agtcgtggcc ctcctcggag aacggggcgc ccgtgttcag
    10081 cggaccgcaa ctgacgacca gcccgccata gaatccgcgg agccagtcca tgccgcggtt
    10141 gtacgcatga gacggggcgt tggtgccggt cagggacagc caagtcagcg actggttcat
    10201 gtattcggcg tcggcgatgt ccaggccgcg atcgatgacc actttgtagc gcagcccgcc
    10261 gccggtattg acccaggcga tccgaacatc gcgcccggga ccattgtcca gaaccgaagt
    10321 ctcgatcccc cccagttgca gcacattcat taccgggacg tcagcgtact tgcccgcagc
    10381 ggtcgtcttc ttggccatga tcatcttcct cctatctcat gatctcattg cctgctcagg
    10441 agcctttggc ggttcgcgac attctaccgg gcacgtcggc aggcggaaac agccagtcac
    10501 atcgcacgaa accctggttt gtgggctgac agacaggcgt cattcggcac ggccgttcgc
    10561 cggaccggca acgcggcgca acacggcacc atccggctca gcggttggcg taccgggcgg
    10621 ccacagtccc cttccagacc agaacgacgt cgagaagcaa ccatgctgca acgggcatgc
    10681 accagaagaa gaactccagc gggtcgttgt ggataaggag aacgcgctgc atggcgagaa
    10741 agacgtccat ggattccacg taaaacgtca ggattgtcca cccgcagccg aacaggagcc
    10801 acaggatcgt cagaaggcca agatgggcct gcatctgcat cgcagccgga agcaggttgc
    10861 ggttgttctg ccaggacagg atccacccca cgatagcggc gatcaacagc gtcgcgccga
    10921 ccacgctgac ggccgaggag atgcctgcca aaggggcagc aacggtgaag aattccttgc
    10981 cgacaatcaa ggagaagtcg tcgtcggaat tcctgaaatg aatccataga gacgcacaga
    11041 aagccgcgct tgcggccagg ataccaacca gcaggggtgc cgccaggttc catcggtacc
    11101 gccgcgacgg ctggttgatc tggatcagcc gcccgaattg acaaggccgc cgcatggcct
    11161 cgatgctgct cttccaccat gcggcaaacc aatgttcctt cgttgaacgg tccagtggtg
    11221 tgcctggtcg gacgtccggc cccagggagt caatagccgg cgtgccacat tccgggcacc
    11281 tgccgtcaag ggcagagccg gtcaggttgt agccgcagaa ctcgcacgtc ggaggacgca
    11341 cgatcgaagg caacttgcgt ggcgcgccga tcgcccgcag cagagaccac agcacccaga
    11401 caacacccgc aatgcaggtc cagcctatga tttccgccga gttgcgaacg atgaaaggcc
    11461 gtgtgctttc gtactcccgc aacgtctgca gccaccgctt gttgtacgag ctgatctgga
    11521 gtgtgtattc ggcttgtgcc gcctggtaat ccttcatggc ctgggaatcg ggcgcggcac
    11581 cggctggata ggtcggcgga gtgggaaacg tgaaactggg gtacgggtgc cgagcctcga
    11641 agctgcgagc cgccgcggag atcgggacga tcatcagccc aatcaacaaa atgagcggaa
    11701 ccgcctgcgt cgagagcagc caggtgtgcc gcagggcatg ggcgtagctg cagcgcaacg
    11761 gttcgtcgcc ggcgccccaa ggtgtcagga tgaacgccgc cgcgaggaaa ccgagttcaa
    11821 tgaagcctgc cgtgatacca gtcaccacgg cggcgatgtc ggggtgatcc atccagaaga
    11881 gcccgaaatc cgcccatggg ctccaactgt cgccggaagc cgtggattca agatcgatca
    11941 tcaacataca ggtcacgaaa gcagcgagtg cgccgagaag atgaaccgtg aagcacgacc
    12001 agaggggcat cctggcgacc acgggcgccc acttggccgg tcttagccac ggcgccacgg
    12061 tcgccagaag cgacccacgc ggtccggtat cggcgacgga atgcatcgac ggcacgtcgg
    12121 ttgtcgcggt ctccggggtc gagtccgtct gcactcttac agtcatgggc cgtcacacct
    12181 cgtgaatgcc gggcgatctt caactgcccc aggcaatcac cgattcgcgt accgcgccgc
    12241 ggccgttcct ttcacaacaa gaacgaagtt cacaacaaca ccggccgcgc agggcaagaa
    12301 ccaacacagg aatcccagca gagcaccgtc gattgacagg gtgtctccca tcgaccggaa
    12361 ggccccttcc atcgccagga gagtacagca cacgagccag acccagacga gcaccgccca
    12421 taccagccag aagccgccga gatagcattg catctgcatg gcggcggggt gcagattgcg
    12481 cccatgacat ctggagagca ctcccccgac gaccgccgcg atgagcagcg ccatgatgag
    12541 gacggctgcc gcgcttgctg cgccgaagac cggcgccgcc agcagtgtcc gcgcgaccac
    12601 aaccctgttg agagcggttt cgggagccat caccgagagg gcaaaagcaa cggcgctcac
    12661 gcaccagaga ggcagcagta tcgccgtgag acatcgactg tgattccgcg aggtctgtgt
    12721 tacccgaatc tgccgaccca gccctgccgg tcgaaaaacc gcgtctcgcc agcatcttcg
    12781 catagccgcc ccgaacgcgg tggggcgcgt cagatcgcag gcagtccctg gtcttgcctc
    12841 ggacccgagc gacatgggta caggtacgcc gcattcaggg cattgcccgt caggcctcgc
    12901 cccggtcaag ttgtaaccgc agtaatcaca ggtcggcggc cgagaactcg gcgttgccgc
    12961 gcgagatgct cctgtcgctc ggaacagcgc ccaaagaaac catgctgaac acgccgcgat
    13021 cacgcagctg acgacatacc caccgtactg ttgggcgtaa tcatgagcga caggatcgcc
    13081 aaatctgagc aggtagttgt actcggtcat cgcagtaccg gatacgaaga tgagcggaac
    13141 cgcatgtgtg gtgtgaagcc agacaagcct gagggcgtgg acaaagctgg cggccgggag
    13201 ttcatccaca gccccccatg gcgtgagcag ccatgccaaa aacaagcacc cagcttccac
    13261 ggcgatcagc agcatgaacg cgccaacgac cactcccaca ggatcgttct gaaacgagtt
    13321 cctgacagtt cccaccgtgc tgaccggcga gccatggagc cgggcggaga agtcggtcat
    13381 caacacgcat gcgacaaaac agccaatcat gccgaggata tgaaccggga aggcctgtcg
    13441 cggcgagact tggcgggcca atggcgccca tgcgaccggt cgtagccatg ggcacagctt
    13501 tgccagaaac tccaatcttt gtggcgtatg aggggcgggt acctcggaca ctggcgttgt
    13561 cgtacttggt ctcccattca cgtgcaaacc ctcgccgagc acccccatcg ccgtcctctt
    13621 gacgagccga ggggtttatc ccctcggtct tcttgacgag ccgaggggtt catcccctcg
    13681 gtcttcttga cgagccgagg ggttcatccc ctcggtcttt aagaaacagg cacattaccg
    13741 gcgggataaa cccgccggct cgtcaagacg gtccccgcca cgggcacact gtccctgccc
    13801 tgatagacgc gccgggcagg cagatgtttc gccgcaccgc cgacagacac cctacgctcg
    13861 ccggttgcgg tcgtccagac gaccgggtac gccattcctc caggcaatgg tccttggcgg
    13921 tccagccggc actctcagag ccgcgaccct ggtttgccga ggcgaacgcg cctgtccggc
    13981 gttgcctgct gggaaaacgg gaagggatgg gccttcgttt ggttttggga cctcacggcc
    14041 ggaggtccgt gtggcttgcg agcgcgtttc cgggtcgcag cgcgcttcgg ccgcgaaccg
    14101 cacgagggcg gtccgtctag aaccttacgg ccagaagtgt gtgcagattg cgggcatctt
    14161 tctgcatcgc ggtgcgcttt ggttgcagct cgcaagaaga ccgtcctcct cagtctctat
    14221 cctcgcgcag ttgccgctcg actttaccca gcgcatcgag gagttcgata cgacgctcgt
    14281 tcttgtcaac gtaaccgggc caagcttttg ccgcggcaat ctcatcgagc tcacgcttga
    14341 gctctcgttt ttgctccaac agccagtccc gctcttccgg ggtcagtgcc atggcttcac
    14401 ctgttctcag tctgtctctt cgtgactggt ctatattctg cgcgcatgtt tcccgatcac
    14461 cgtgcgattt ggctgcggcc cgcaaaaagg cggtccgcct tggagttgcc cactctggta
    14521 cattatacag cctgaagctc agtactggaa gatgacactg ctgcatacgt tcttgccatt
    14581 ggtatccacg tgctcgagat ccaaggcttt gaccgaaact gccttcctga cgctttccga
    14641 gggcatcgcc ttgacacgga ttgtcacggg ccgtgacgcg gcgatgatat cgtcagagac
    14701 gaccggcgaa aaatcgacta atggcggtca tcggcgtgcc ggacgagcgg cgagcgtcaa
    14761 gccgaccgat gccgacatgg caccgcttta tcaggaagtg gagaaagcaa taccctggat
    14821 gcccggcgag cgccaatgag tcagcgactt tcccaaccgc cgcgaatgct ccaaggcctt
    14881 gcccagcacc atccggcaaa tcataatcag gccacgttgg agtgttcctg accgtgcgtg
    14941 actgggcaaa tcatgacacg gaccgggagg acagcccatt tgagaaaggc cgggaaaatg
    15001 ctgcatgctt tagctctatc ggcggttctg accgtcagcg cggttccgga tgagttcatc
    15061 gttctatcgg agttccaggt gtgggcagac ccggccggct ccgaggtgtt caaagaactt
    15121 aatcgtcggt acctcaagac cggccgcagc cacgtcttca atctctatcc ccgacacgac
    15181 tacttcgtct gggagctgcc gaagacgcgc ggttgggtgg atgaagccat tatccttggg
    15241 gctttcaacg tattctgcat cggcgacgac attcgtaccg cagaagggtt cttgtttgat
    15301 gaaaaaggtc tcagcccgcg attcaaggat gtcctgttcg gcaccatcgc ctacgctcac
    15361 gaaaagggat tgatggtcgc cgtcgagccc caaggactgc cgaccaccaa agacgacgaa
    15421 cacctgcaac gctggttcag aagctggctt ggcccggata tcccaaggga tcagcaagcc
    15481 gatatcctca aactcagcct cgactggttt ggcgccaatc aattcaaccc gcagcttgcg
    15541 gaggagattg aagcggtctt cacggcgtgc cgggcggtca acccggatgt gctcatctac
    15601 ctggattccg tccatggcat ctggcgcaca ccgacgccct ttcatcgctg gctgctccag
    15661 cggtttccgc agactatcat cagccactac ttgaacactg atcagatcga cgcgttccgc
    15721 aaggccggtg ccggcaatat gatggtgcag atcaacccca gcgaaataga agaggtgggc
    15781 ggccagtttt tcatctttca cgacaagacg gtcgcctggc ttcaggatgc ggtgcgaaga
    15841 cgcgtacgct atctgtcgct ggccggcgtg aattatggtt acaaccgata taactacgac
    15901 ctgttcctcg acgtcatccg gcctcatttg gcactgaagc gcaatcttca agcggtacgc
    15961 gagagcttgc ttccggaccg gattgcccaa ccgacgacca aggaggatgt cagggcatgg
    16021 ttgcttgaac aatcgaacaa ggggaagtga tcgaattcag ggccccgatc agcgaagcgt
    16081 tgacatccgt ctgtcggctt tcgcgttgca tgaaaagacc aacggcgaac ccgggaaagt
    16141 acttcccgtt gcgttcgccg ttgtcatctt tatgatggcg cttgggttcg tcggcttgca
    16201 cgaatccagt gcccgggccc cttcggggct cactaaatgc ggctgccgct tttcacggcg
    16261 caatcgggtt ttcgaccctc gaccagcaga atcctccgtc tattcagaga actcacgggc
    16321 cgatgccagg acggcacatg ccgccccggc cccgtcagtt atcacgtgta tgcccaacaa
    16381 ctgtcatcgg acaattgccc ctccgaagtt cagcgttttc accgttctca catgttcttc
    16441 cggtcagcct ccggcgacgg ccggatcaat gaagcacccg tgcatacgcc ggggtactca
    16501 tttcgcgagg cgctccggcc aacctgtgga ccttttatcc tgctgaaaaa cctgggctac
    16561 gcatcccact tcccccacat ccgcattgat tggcgacgcg gccactggca agacgcatcg
    16621 atctgcgaat cgtcaagatc tcaatggcca cggccgctcg gttaacgatt ggttcaacga
    16681 ctggcttgga acagaccggc cctgacattg gcacatgacg ggccttctcg gcggatgtgc
    16741 cctccgtcgc tcttggagct atggaggaca ggcccgcaac gaccttcgct tggcggccgg
    16801 cgcgctgtcc ggcgggactt caggcttcag gctggagact tcagtctctt ccgtcgcgac
    16861 tggtgcgcat tttgcgcgca tgtttccgga tcgcagagca cttgggttgc ggcccgcaaa
    16921 aaggcggtcc gcctcgggaa ttattacaga agagctgcgt ccccttatcc gcgcgactgc
    16981 atccgaaaaa ctacctccgt ccccgtttcg atacgcgtcg tttcgatagg ccgctcggtg
    17041 ggttcgtttg gtcgcagaga gttcttgagg tgttggctcc tggctttcgg ccggaggggg
//
LOCUS       DAOTPW010000119        16729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3432_length_16729_cov_5.122466, whole genome shotgun sequence.
ACCESSION   DAOTPW010000119 DAOTPW010000000
VERSION     DAOTPW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16729
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_16729_cov_5.122466"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="P5316_11985"
     CDS             complement(<1..900)
                     /locus_tag="P5316_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRR85718.1"
                     /translation="MPADILVTGIGIVSPAGIGCEQTWRTLLANGKAVRRLAGGWHPR
                     FATVCGATIDAFNPPPGTEHLSRTSQLAVAAAEEALAWAKLAGQPAASGRVAASIGTS
                     KPLVDLLLPDPCDADPDRRLRIRPSCGWFDFLPDAPARTVSARFGFARAHSTVCACST
                     GLHAIIRGAEMIQEGQADIVLAGGADFSLSPLWLGAYQNMGVLAPEDPELGPAWACRP
                     FDRQRKGFVVGEGASFVVLESRASANRRGVSPWACLRAWAIGSDPAGLTRLSGDGNPL
                     AEVVRTALNNGSTRPEDVSCIYAH"
     gene            complement(904..1935)
                     /gene="bioB"
                     /locus_tag="P5316_11990"
     CDS             complement(904..1935)
                     /gene="bioB"
                     /locus_tag="P5316_11990"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRR85719.1"
                     /translation="MSDLIRTIVRQVVDGAAVTRSQAEQLVRIDRRHLPELFAGASRI
                     RQEYLGPKARCCSIVASKVGLCDQDCAFCSQSAYYRTHVAGGRTLTSQDVLAAARESA
                     DNGAQSFGIVNSGLGPSDDEIEEWGNVIRAIRREGRVRACASLGVLTAPQAQRLAEAG
                     VQRYNHNLQTSRRHFSSIVTTHTYDDRLQTLRNLRAAGIELCCGALFGMGETWNDRLD
                     LAFELREVNPEVVPINFLIPIPGTPLEHSRALEPIECLRIIAVYRFILPSQHLNIAGG
                     REVNLRDLQSWMFLAGADSFMMGNYLTTCGRPVAEDLRMISDLGLELEPYLQPAAAPD
                     EAHRSHTPV"
     gene            complement(2096..2419)
                     /locus_tag="P5316_11995"
     CDS             complement(2096..2419)
                     /locus_tag="P5316_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85720.1"
                     /translation="MKMNSKDFAIGVLTITAVILLTGLIIIHAVSPRQAMAVGQNAEG
                     GDYLVTTSQYNDYTELLMVFDTAQMKMNAYVFNPQTGQVELLQPPIPIVRQMQEPQRQ
                     PGRTR"
     gene            complement(2427..2717)
                     /locus_tag="P5316_12000"
     CDS             complement(2427..2717)
                     /locus_tag="P5316_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85721.1"
                     /translation="MSKQAFTVFLVGVNLLLLGGLILSSYSLPEAKAQPVSLRGEFIL
                     ISAQAERNNDTIYLLDLARRQMHAFRAQVPQMIEQPMMVRWYHSRDLVRDFR"
     gene            complement(2764..2961)
                     /locus_tag="P5316_12005"
     CDS             complement(2764..2961)
                     /locus_tag="P5316_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85722.1"
                     /translation="MIRTTLFFLTGLLLVLGPALAQGPSEGPTYNFKPRTTMIEWLIG
                     TIFLVGCLVVAFKPSKRSNLK"
     gene            complement(3029..3598)
                     /locus_tag="P5316_12010"
     CDS             complement(3029..3598)
                     /locus_tag="P5316_12010"
                     /inference="COORDINATES: protein motif:HMM:NF038001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HRR85723.1"
                     /translation="MARKSHHYESAFEDYLRVRNIPFVSVDETRRVVFAGTRIKSFDY
                     VVYPPAGRAWLVDVKGRHFPYVDEQGGGKRYWESWITREDLAGLADWESAFGDEYEAR
                     LVFAYLLQGPPDRWPAVMPHLFKGESFAFYTAGLADYREVCRDRSDRWQTVSVPNTAF
                     RRILRPLDALLTGSPPLCPSAERPADLLP"
     gene            complement(3613..4935)
                     /gene="hisS"
                     /locus_tag="P5316_12015"
     CDS             complement(3613..4935)
                     /gene="hisS"
                     /locus_tag="P5316_12015"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRR85724.1"
                     /translation="MRFQAPKGTRDFYPEDMAARNWIMDTWRRVSLRNGFEEYDGPIF
                     ELLDLYKAKSGEGIVSELFHFEDRGGRELAIRPEMTPTLARMIAARANSLPRPIKWFS
                     MPRLCRAERPQRGRLREFFQWNIDLVGVDDVVSDAECILVAIDFFREIGLTPDDVVMK
                     INSRSLLSSLLAAQGFEKQRHEDVFAVLDKRDKLPPEAFVEAVEKVARNANEKAALLE
                     IGQAAGDEGLADLAGVALENQPAQAELARLKQLLGMLARMGVGGYCRFDMGVVRGLAY
                     YTGVVFEAFGKGEFQRAICGGGRYGFLLEAVGGPPMSGIGFGTSDVVILTLLTELNRL
                     PAEAKKIRQVDLFLIDADPTLFDEVLALAARLRKAGFAAEFSYRRQALGKQLKQASAC
                     NASRAVIVGSEYTQRQVVCVKDLASGRQTEVGIERFLADPLDRRSSNS"
     gene            complement(4968..5468)
                     /locus_tag="P5316_12020"
     CDS             complement(4968..5468)
                     /locus_tag="P5316_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRR85725.1"
                     /translation="MKPPTVPPSTDLCGKCVGLCCRYFALAIDKPETRRDFEDIRWYL
                     LHEDTAVFVEEGQWYLQINRKCRALMPDNRCAIYEERPPICRSYKTHNCDWHGDQYEY
                     ERVFVDPDDMTAYAREYLSKKRKSRAAARKRRKATAARKARQLKVRKTGPARRPRIAI
                     PLLKSA"
     gene            5600..6250
                     /locus_tag="P5316_12025"
     CDS             5600..6250
                     /locus_tag="P5316_12025"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRR85726.1"
                     /translation="MRIIAGQWRGRQIAGPEGNRTRPITDRAKTVLFDVLGHRLARPG
                     TLPPAAVLDLFAGTGTLGIESLSRGARFTLFVEQHRPAVALIRENLDSLGVSQARGRV
                     LRADATECEFPPPPGPPDVYSIVLVDPPYRLLTGPSPHPAMRPLLQRLAVDPVIADDA
                     MIVIRHQRNPTGGPDLSPLVELERRDVGSMTFRFMSTPRCGAAVRSESVSGDEEEE"
     gene            6247..7500
                     /locus_tag="P5316_12030"
     CDS             6247..7500
                     /locus_tag="P5316_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRR85727.1"
                     /translation="MKRPLSIQDAALRVVKRLRQHEHQAFWAGGSVRDMLLGREPTDI
                     DVATDALPDTIIKLFTRTRKVGAKFGVVMVRQGPHWIETATFRTDLDYRDGRHPGKVV
                     FTTAEEDAQRRDFTINGLFYDPISKEVIDYVGGRRDLEAGIIRAIGDPARRFAEDHLR
                     MLRAVRFSARFGFPIEPGTAAAIREQAEHIGRISPERIREELEKMLSHPSRAEAVRQI
                     AELGLLDHLWPNGRWPRDRVADSIRVLSKLPEQAGFVLSMAALLNAYPPASVRQIARD
                     LRCSNHQSGRITWLIEHVDDIHHAEVLCLPAMKMLIAHEGFEELLELHQAICLARGLP
                     LDGNEIARRRKASIPPEEVAPPPFVTGDDLIAAGLEPGPRFREILDRLYDEQLDDRLM
                     SRDQAMARMRELLRGTAGEHGHSHQ"
     gene            complement(7639..8643)
                     /gene="xerD"
                     /locus_tag="P5316_12035"
     CDS             complement(7639..8643)
                     /gene="xerD"
                     /locus_tag="P5316_12035"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRR85728.1"
                     /translation="MSHVPFTIGSEQPRSESVPDLRLRSWPQPESRAAPALSWCAQIE
                     AFLRYLKIEAGLSENTLTAYRHDLTSLAGFCADQRIDIKTINVRHIQAFMVALRESVN
                     LAESSIARRLVAVKLFLRYLHQHGLTDEDVSALVETPKKWQHLPRVLSRQDVDKLLGL
                     PNEADDPLALRDRAILELFYATGLRVSELVGLVLGSVHLDIGYLRCMGKGRRERVVPI
                     GSRAIEALREYLTILRPQLVDGTSSDRLFVSRTGKPLDRTNCWRLVVKYARQMGVAGR
                     LSPHTLRHCFATHLLAGGADLRVVQELLGHADISTTQIYTHVDNSRLKAVHRQFHPRQ
                     "
     gene            8959..11400
                     /locus_tag="P5316_12040"
     CDS             8959..11400
                     /locus_tag="P5316_12040"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR85729.1"
                     /translation="MSRRLTDVAVIALVLLSAGGRPVSAAAKELTYEDLLAHLTDLDR
                     LPVIEPGVRCLQFSSYDRASQYDAATNSYINWDANGDAGNYLRIDPQTKEGVMAEMDG
                     PGCIFRIWSANPQGVIRFYLDGDAKPTYEWDFSKLCTGVIAPFVRPLVWKRDPNDVNS
                     ASNIYLPIPFAKSCKVTSVIPQPDGSGKTPGHYYIIDYRTFPKDWTVPTFKLPLTESQ
                     RAAVEETALKWSRCGQTGAHPLDRFATKKETIAPGQRLVVAELEGPAVIRQFRAKVNS
                     SEKWATRKVGLRIYWDDETEPSVCCPIGDFFGEPKDVEYKSYPMGITDSMNYCFFPMP
                     FRSRAKIDLVNEGKQPAPVEVAVAYRTQEVPEDWAYFKAKFRCEIASESFDYPLIETS
                     GTGKLVGICLFPDNIHGGWWGEGDEKVYVDGEKFPSWFGTGSEDYFGDAWGIRHFTNP
                     SHGHPQKNVERMQGCYRWHLGDNIPYYKSLRMTIENYTGLPHVTSRNDYSSVAYWYQL
                     PGGEDFFEEVPLVDRIPRGFVATGALEIERCQDPAALSDSLRLVDDAGLPKPLSSGGG
                     LMFVGKLGDEVTFQVPVAEDEHYLLRPVLAQDVRNSRFELLVDGKPAGERPYLRKGVM
                     PITLKFTGEPVDGDRCRVIIDYMRMEVYRNFITQWLVVGPFPNPEHKGFSTVYPPEER
                     LDFGARYAGNDNQELTWQPVSSQDGVIMLQGRFKPSEDVVVYGACIVRSPKAGKRTLL
                     VGSDDGIKIWLNGKLVWEHLVRRAIKPDEDRIEVELKQGDNLLLLKLDQGPGDMGWAV
                     RFLDPADELQFALPK"
     gene            11621..13150
                     /gene="rfaE2"
                     /locus_tag="P5316_12045"
     CDS             11621..13150
                     /gene="rfaE2"
                     /locus_tag="P5316_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRR85730.1"
                     /translation="MVAIRRCCGEGRELIPRLIQLVQSFGRQRVLLIGDLILDRYVYG
                     DAERISPEAPVVVLRKEHDEERVGGAGSVAAGMRELGLEVVIVGAVGLDDAGHRIRAL
                     LEAQRIGTRGLIALSDRPTTTKTRFIGLAQHRHRQQLIRVDDEVTHPLSEPDAARVTQ
                     MAVAGVKDVAAVCLEDYDKGLLSESLCQKVIAVASAAGKPVLVDPARLGDYSKYRGAT
                     LLTPNRAEFQLASGCRDTSVETILVSAHGVIEKYDLGGLLVTVDRDGAILAEKGRKAV
                     HIATRPRAVYDNTGAGDAVLAMLAAAIVAGATWEEAARLTNIAGGLEVERFGCVPITQ
                     EEVIADLRLSAGAGTGKIRTADELVTELSLRRSRHETVVFTNGCYDILHAGHVRFLEQ
                     CKALGNIVVVGLNSDASVRALNKGKDRPIVPERHRAEVIAALQSVDYVVLFDEPTPLR
                     LIEKIRPEVLVKGEDWKDKGVVGREFVESQGGKVILLPLVEGLSTTRLIERIRAGAYD
                     E"
     gene            13296..13829
                     /gene="pyrR"
                     /locus_tag="P5316_12050"
     CDS             13296..13829
                     /gene="pyrR"
                     /locus_tag="P5316_12050"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRR85731.1"
                     /translation="MKSSSSEVLLDAAGIAAVLERMAREIFDSLPEGVPVAIIGILRR
                     GEVLAQRIQTALLRLGARDIRHGTLDITLYRDDLASIGSQARLRQTAIDFDIEGVYLI
                     LVDDVLYTGRSIRSAMDALVDLGRPKAIRVAVLVERPGRELPIQPDFVGVRIGREDMP
                     VTVSLAESDGIDEVRVG"
     gene            13826..14827
                     /locus_tag="P5316_12055"
     CDS             13826..14827
                     /locus_tag="P5316_12055"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRR85732.1"
                     /translation="MKTDTRTAAAPAGAGPAPKPEPEFTWARRHLLGLEDLNREEILH
                     IFRTAESLQDISTRSIKKVPSLRGRVVVNLFFENSTRTKTSFTLAAQRLSADVIDFTA
                     ASSSVAKGESLRDTVRNIEAMGVDTFIIRHTAAGAPHLITRCTQCHVINAGDGQHEHP
                     TQGLIDIFTIRERKKRIEGLNVVIVGDIAHSRVARSNFWGLTRLGAHVTFCGPTTLVP
                     QAFEKFGARVTHNLDEAIRDADVINMLRIQHERMTSSVFPSTQEYVRLFGLTSERVRH
                     CKPDVLILHPGPINRGVELASDVADGPNSSILRQVSNGLAIRMAVLFLLNQTSEAAK"
     gene            15154..16563
                     /locus_tag="P5316_12060"
     CDS             15154..16563
                     /locus_tag="P5316_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85733.1"
                     /translation="MRRVFTFLTWGGIVIGLTLAGVTCNAGNDGADNGGGDGNGGGGG
                     DGPAHLVEVPDPLSGDNPRYPLVTKSVPAAGGSVTDAAFGIAQTRVVQTEGLRHEYSR
                     LDPFNSDRTLILLLLTSAGEWRVYRTQSVPYDQSGNLVRTLDLAEPRWDPSDANLVWG
                     LRDFQIVTVNVQTGDETVVKDFTQDATIAPILTANPDLYRITMKDEGESSTDNRYWAF
                     MLQGTADDYRPRYLLTWDRQQDLVLGTWAIPLNESRIDWVGMSPLGNWVVIGADWDNG
                     GGKSGMVIADRGFSQFHQIDYNAGHSDVGLDSEGNEVIVLQSNRTDSIDMLPLSPNTE
                     PVDTGESYAGTGRVQVIRLFYDSSSPLGLNSGIHISCNAPGWCVVSTFTEPGLPEQNW
                     LDRTITLVRLDPSHPRVFYLAKVHGTAGAYWEETQASITRDGSRVVWATNWNRNVGQE
                     RVWLIQLEIPDEAMASIGG"
BASE COUNT         3164 a         4934 c         5434 g         3197 t
ORIGIN      
        1 gtgggcgtag atgcaggaga catcttcggg gcgggtagat ccgttgttca gggctgttcg
       61 gacgacctca gccaaggggt ttccatctcc ggaaagccgg gtcaggccgg cggggtcgct
      121 gccgatggcc caggcccgca ggcaagccca gggtgaaacg ccccgccggt tcgccgaggc
      181 gcgggactcg agaactacga aagatgcgcc ctcgccgacg acgaatcctt tgcgttgccg
      241 gtcaaagggc cgacatgccc aagcaggtcc cagttcgggg tcctccgggg ccaggacgcc
      301 catgttctga taagcgccca gccacagcgg cgacaacgaa aagtcggctc cgcccgccaa
      361 gacgatatcg gcttggcctt cttgaatcat ttcggccccg cggatgatgg catgcaggcc
      421 cgtcgagcag gcacacacgg tcgaatgcgc gcgggcaaag ccaaagcggg cagagaccgt
      481 gcgagcgggc gcatctggca ggaaatcgaa ccatccacaa gacggtcgga tacgaagcct
      541 cctatcgggg tcggcatcgc agggatccgg caaaagcaga tccacgagag gcttgctcgt
      601 gccgatggaa gccgcaactc gcccacttgc tgcaggttgg ccggccagtt tcgcccatgc
      661 cagggcctct tcggcagcgg cgacggccag ttgggaggtc cggctgagat gctcggttcc
      721 tggaggaggg ttgaaggcgt cgatggtcgc gccgcacaca gtggcaaatc ggggatgcca
      781 cccccctgcc agacggcgaa ccgccttgcc attagccaac aatgtgcgcc atgtttgctc
      841 gcagccgatt ccagcgggcg agacgattcc gattccggtt accaggatat cagcgggcat
      901 ctttcagacg ggcgtgtgag agcgatgagc ttcatctggg gctgcggccg gttggagata
      961 aggttcaagt tcgaggccga gatcgctgat cattcgcagg tcttcggcaa ctgggcggcc
     1021 gcaggtggtc aggtagttgc ccatcatgaa gctgtcggcc ccggcgagaa acatccagct
     1081 ttggaggtca cgcaggttaa cctccctgcc gccggcgatg ttgagatgct gcgatgggag
     1141 gatgaaacgg tagacggcga tgatccgcag acactcgatc ggttcgagtg ccctgctgtg
     1201 ttcaagcggc gtaccgggga tcggaataag gaagttgatg ggtacgacct cgggattgac
     1261 ctcgcgcagc tcgaaggcca gatcgagacg atcgttccat gtctcgccca tgccgaagag
     1321 agctccgcag catagctcga tgccggccgc ccgcagattt cgcagcgttt ggagccgatc
     1381 atcgtatgta tgcgttgtga cgatgctcga gaaatgtcgt cgtgaggtct ggaggttgtg
     1441 gttgtatcgt tggacgccgg cctcggccag acgctgagcc tggggagcgg tgagcacacc
     1501 gagtgaggcg catgcgcgca cccgcccttc ccggcggatt gcccggatga cattacccca
     1561 ctcttcgatt tcatcatcgc tcgggcccag gccgctgttg acgataccga acgactgggc
     1621 gccgttgtcg gcagattccc gcgcggccgc cagcacatcc tgcgacgtca gggtcctgcc
     1681 tcccgctacg tgggttcgat agtacgcaga ctggctgcaa aaggcgcagt cctgatcgca
     1741 tagccccacc tttgacgcaa cgatgctgca acagcgtgcc ttggggccga ggtattcttg
     1801 gcggatccgg cttgcgcccg cgaataattc gggcaggtgg cggcggtcaa tccgaacgag
     1861 ctgctcggcc tgagaccgcg taaccgcggc gccgtcaacg acctgtcgaa cgatcgtgcg
     1921 tatcagatcg gacattgtgc ggtgttccga tgtgtcaatg cgggttcgcg ccgtcctgtg
     1981 ccgttcccgg cggtttggcc gtgttccggc gggccggcca cccgtgaaac ccgcggattc
     2041 ggcagcatct gccggtcgac aggaatagcc ggatgctggg gcaggcatgc ggctctcatc
     2101 tcgttcgtcc gggctgtctt tgaggctcct gcatctggcg gacaatgggg atgggcggct
     2161 gcaggagctc gacctgaccc gtctgaggat tgaagacata tgcgttcatc ttcatctggg
     2221 ccgtgtcgaa gaccatcaac agttcggtgt aatcgttgta ctggctggtc gtgaccaggt
     2281 aatcgccgcc ttcggcattc tgtccgacgg ccatggcttg cctggggctg acggcgtgga
     2341 tgatgatcag gccggtcagc aggatgacgg cggttatggt caggacgccg atcgcgaaat
     2401 ccttgctgtt cattttcatc cccctttcag cggaaatccc gcaccaggtc gcgcgagtgg
     2461 taccaacgca ccatcatcgg ctgctcgatc atctggggca cctgagcgcg gaaggcgtgc
     2521 atctggcgtc gtgcaaggtc gagcagatag atggtgtcgt tgtttctctc cgcctgggcc
     2581 gagatgagaa tgaactcgcc gcggaggctg acgggctgag ctttggcctc gggcaacgag
     2641 tagctcgaca gaatcaggcc gccaagcagc aggagattga cacctacgag gaaaacggtg
     2701 aaggcttgtt tgctcattct caggatcctc ctcagtaaga gcgttcaggc cgtcgcatcg
     2761 cgatcatttc aggttcgatc gcttgctggg tttgaaggca accacgaggc agccgaccag
     2821 aaagatcgtt ccgatcaacc actcgatcat cgtcgttctg ggtttaaagt tgtaggtcgg
     2881 tccttcggat ggtccctgcg ccagagccgg accaagcacc agcaacagtc ctgtcaggaa
     2941 gaacaacgtt gttcgaatca tgaatcttgt cccgtaggcc gtagtttcca cattccccgg
     3001 tattgacttc attataggca agggtgattc agggtaaaag gtcagcgggt ctttcggccg
     3061 acgggcacaa tggcggggat ccggtcaaga gagcgtccag gggccggaga atccgccgaa
     3121 aggcggtgtt gggcacggat accgtctgcc agcgatccga gcgatcacgg cagacttcgc
     3181 ggtagtcggc caagccggcg gtgtagaaag cgaaggattc tcccttgaac agatggggca
     3241 taaccgccgg ccagcggtcc ggcggcccct gcagcaggta tgcgaacacc aaccgggctt
     3301 cgtattcgtc accgaacgcc gattcccagt cggccaggcc agcgaggtcc tcccgcgtga
     3361 tccagctttc ccaataacgc ttgccacccc cctgctcatc gacgtatggg aaatgccggc
     3421 ccttgacgtc gaccagccaa gctcgtccgg ccggcggata gacgacgtag tcaaaggatt
     3481 tgattcttgt tcccgcgaac acgacccgcc gcgtttcgtc cacggacacg aacggtatgt
     3541 tcctgactcg gaggtagtcc tcgaaggccg actcgtaatg gtggctctta cgagccatga
     3601 ttgtcctgct ccctagctgt ttgacgagcg ccggtcaagg gggtcagcca agaagcgctc
     3661 gatgccgact tcggtttgcc ggccgctggc caggtctttc acacagacaa cctgtctttg
     3721 cgtgtactcg cttccgacga tgacggctcg cgaggcgttg cacgcggagg cttgcttgag
     3781 ctgcttgccg agggcctggc gacggtaact gaactcagcg gcgaaacctg cttttcgcag
     3841 ccgggctgct aacgccaaca cttcatcgaa caaagtcggg tcggcgtcga tcaggaagag
     3901 gtcgacctgc ctgatcttct ttgcctccgc aggcaatcgg ttcaattcgg tcagcagagt
     3961 caggatgacc acgtcgctgg tgccaaatcc gatgccggac atgggcgggc cacccaccgc
     4021 ttcgaggagg aaaccgtacc ggccgccgcc gcagatcgct cgctgaaact cacccttgcc
     4081 gaacgcctcg aacaccacgc cggtgtagta tgccagaccg cgaacgacgc ccatgtcgaa
     4141 ccggcaatac ccgccgacgc ccattctggc cagcatcccg agcagttgct tgaggcgggc
     4201 caactcggct tgggcgggtt ggttttcgag tgccaccccg gccaggtcgg caagaccctc
     4261 gtcaccggcg gcctggccga tctccagaag cgccgccttt tcgttagcgt tgcgggccac
     4321 cttctcaacg gcctcgacga aggcttccgg aggaagcttg tcccgcttgt caagaactgc
     4381 gaagacatcc tcatgccgct gtttttcgaa gccttgggcg gcaaggagcg acgacagcag
     4441 ggaacgtgaa ttgatcttca tgaccacatc atcgggcgtg agcccgattt cgcggaagaa
     4501 gtcgatggcc acgaggatgc attcggcgtc gctgaccacg tcgtcgactc cgacgaggtc
     4561 gatgttccat tggaagaact cgcgcagccg gccccgttgc ggacgttcgg cccggcacag
     4621 ccttggcatg ctgaaccact tgatagggcg cggcagcgag ttggcccgag cggcgatcat
     4681 tcgggcgagc gtcggtgtca tctcgggtcg gatggccaac tcgcgtccgc cgcggtcctc
     4741 gaagtggaac agttcggaaa cgatcccctc gccgctcttg gccttataga ggtccagcag
     4801 ctcgaagatc gggccgtcat actcttcgaa tccgttgcgg agcgagaccc ggcgccaggt
     4861 gtccatgatc cagttgcgcg cggccatatc ttcggggtag aaatctcggg tgcccttggg
     4921 tgcctgaaac ctcatgttct tcactttcgg ccgacggaga agacggttca cgcggacttg
     4981 agcaacggga tcgcgatgcg cggccgtcgg gccggcccgg tcttgcgaac cttgagctgc
     5041 cgtgctttcc gggcggcggt ggccttccgg cgtttgcggg cggctgcccg gctctttcgc
     5101 tttttcgaca agtactccct ggcgtatgcg gtcatatcgt cggggtcgac gaaaacacgt
     5161 tcgtactcgt actggtcgcc gtgccagtcg caattgtgcg ttttgtacga cctgcaaatg
     5221 ggcggtcgtt cttcgtagat cgcgcaacgg ttgtcaggca tcagggctcg gcacttgcgg
     5281 ttgatttgca gataccactg gccttcctcg acaaacacgg cagtgtcttc gtgcagcagg
     5341 taccagcgga tatcctcgaa atcccgccgt gtttccggct tgtcgatggc caaggcgaag
     5401 taccggcaac agaggccgac gcacttgccg cagaggtccg tcgaaggggg gactgtcggt
     5461 ggtttcataa tgcggttgtc tccatgacca aagcggtgat tgtacgaaaa aggcgtcttt
     5521 gcggcaacat cgactcgcgc ttaacctcct ctgttcggcg gcgttgccat gggcaacgtc
     5581 gggggatacg ctggttttca tgcggatcat tgccggacaa tggcgaggac gtcagatcgc
     5641 cggtcctgag gggaaccgga cgcgtccgat caccgatcgt gccaagacgg tgctgttcga
     5701 tgttctcgga catcgccttg cacgcccggg tacgcttccg cctgcggcgg tcctggatct
     5761 cttcgccggc acgggcacgt tgggtatcga gtcgttatcc agaggggccc gtttcacatt
     5821 gtttgtcgag caacaccgcc ccgccgttgc tctcattcgc gagaatctcg attcactggg
     5881 cgtctcgcag gcccgggggc gggtgctgcg cgccgatgcg acggaatgcg agttccctcc
     5941 gccgccgggg ccgccggatg tctattccat cgtgctggtt gaccccccct accgtctgtt
     6001 gaccggacca tcgcctcatc cagcgatgag gccactgctt caaaggctgg ccgtcgatcc
     6061 ggtcattgcc gatgatgcca tgatcgtcat ccggcaccag cggaacccca cgggcggtcc
     6121 ggacctctct ccgctggtcg agctcgaacg gcgggacgtg gggtcgatga ccttccgctt
     6181 catgagcacg cctcgttgtg gggcagcggt ccggagcgaa tccgtatcgg gggacgagga
     6241 agaagaatga aacggccgct gtcgattcaa gacgccgcct tgcgggtggt gaaacggctt
     6301 cgccagcacg agcatcaggc tttctgggcc ggcggcagcg tgcgcgacat gttgctgggt
     6361 cgggagccga cggacatcga tgtggccacg gacgccctac ccgacacgat catcaagctg
     6421 ttcacgcgaa cacgcaaggt cggagccaag ttcggcgtgg tcatggtccg ccagggtccg
     6481 cattggatcg agaccgcaac gtttcgcacc gacctcgatt accgggacgg gcgtcatccg
     6541 gggaaggtcg tcttcacaac agccgaggag gacgcccaac ggcgtgattt tacgatcaac
     6601 gggcttttct acgacccgat cagcaaggag gtcatcgact acgtcggcgg gcgaagggac
     6661 ctggaggccg gtatcatccg ggcgattgga gatccggccc ggcggtttgc cgaggatcat
     6721 ttgcgcatgc ttcgagcggt gcggttctcg gcccggttcg ggtttccgat tgagccgggc
     6781 accgccgccg ccattcggga gcaggccgag cacatcggtc gtatcagccc cgagcgaatc
     6841 cgcgaggagc tggagaagat gctgtcgcac ccgtcgcggg ccgaggcggt tcggcagatt
     6901 gcggaactgg gccttctgga tcacctgtgg ccgaacgggc gttggcctcg tgaccgcgtg
     6961 gcggacagca ttcgcgtgct gagcaagttg cccgagcaag cgggtttcgt cctgagtatg
     7021 gccgccctgc tgaacgcgta tccgccggcc tccgttcgac aaatagcccg agatctgcgc
     7081 tgcagcaatc accagagcgg ccggatcacc tggctgatcg agcacgttga cgacatccat
     7141 catgcggaag tcctgtgcct gccagccatg aagatgctca tcgcccatga gggtttcgag
     7201 gagcttctgg agttgcatca ggcgatctgc ctggctcgag ggctgccgct tgacggcaac
     7261 gagatcgccc ggcggcgaaa ggcgtcgatc ccgcccgagg aggtggcccc cccaccgttc
     7321 gtcaccggcg atgacctgat cgccgcaggc ctggaacccg gcccgcgatt cagagagatc
     7381 ctggaccggt tatacgacga gcaactggat gatcggctga tgtcgagaga tcaggcgatg
     7441 gcgcggatgc gggagctgct taggggcacc gccggtgagc acggacatag tcaccaatga
     7501 cccagtaccg ggggccgaac agaaccgaat aaggaagtct cgaacagcag atgcgccctt
     7561 tggcaggccg cagcgctgcc gcgtcctgcg aagaaagcag gtttcacggc cggggttgcc
     7621 aaagtccgat gtcgcaggct actggcgcgg atggaattgg cggtgaacag ccttgagccg
     7681 cgagttgtca acgtgcgtat agatctgggt cgtgctgatg tccgcgtggc cgaggagttc
     7741 ctgcacgacg cgcaggtccg cccctcccgc cagcaggtgc gttgcgaaac agtgccgcag
     7801 cgtgtgcggg gacagcctgc cagccacgcc catctgtcgt gcgtacttga ccacaaggcg
     7861 ccagcagttc gtgcgatcca gcggcttgcc ggtccgtgaa acgaaaagcc ggtcgctcga
     7921 tgttccgtcg accaactgag gccgcaggat ggtcaggtat tctctcagcg cctcgatggc
     7981 ccgggagccg atcggcacca cccgctcgcg gcgccccttg cccatgcagc ggaggtatcc
     8041 gatgtcgaga tggacgctgc cgaggaccaa gccgacgagt tcgctcaccc gaaggccggt
     8101 ggcataaaac aactcgagga tggctcgatc gcgcagggcc agcgggtcgt cggcttcgtt
     8161 gggcagaccc agaagcttat ccacgtcctg tctgctgagc acgcgcggca ggtgctgcca
     8221 tttcttgggc gtctcgacca gcgcggagac atcctcgtcc gtcaggccgt gttgatgcag
     8281 gtacctcaga aagagtttga ccgccacaag gcgccgcgcg atggacgact cggcgagatt
     8341 gaccgattcg cgcaaagcaa ccatgaacgc ctgaatatgg cggacgttga tcgtcttgat
     8401 gtcgatgcgc tggtcggcgc agaagccggc aagggacgtc aggtcatggc gataagcagt
     8461 cagtgtgttt tctgacaggc cggcctcgat cttgaggtac cgcaagaagg cctcgatctg
     8521 agcgcaccag gacagcgcag gcgccgccct gctttcaggc tgaggccaac tgcggagtcg
     8581 caagtcgggc accgattcgg atctcggctg ctcggatcct atggtaaatg ggacatgact
     8641 catggtcatg ggcgcacaag cggaacactt cccgcagatt ggtcatggtc cacaggccgg
     8701 cacagcgcgc atcgccgctg tcgagatagg gacacacaga ctgcggggtt gtcacgggtt
     8761 cgtcctcact cacatacggg cgcggtcgtc ggagcgtcgc ccgtcgcgat aattatcggg
     8821 cgggcatggg attttgcttg agcggatctg ggcgatcgcg aattccgtcg cggcagttgc
     8881 gatccggccc gggcaggctt accatcacgc cgcaaaacgg tgcggacgtt tactgttcct
     8941 tcctgggagc taagtgccat gagtaggaga cttacagacg ttgcagtgat cgcgttggtg
     9001 ctcctttcgg ccggcggtcg gccggtttcg gcggcggcca aggaactgac ctatgaggat
     9061 ctgctggccc acctgaccga tctggaccgt cttccggtga ttgagcccgg ggtgcgttgc
     9121 ctgcaattca gcagttatga ccgggcaagc caatatgacg ccgcgaccaa cagctacatc
     9181 aattgggacg ccaatggaga cgcgggcaac tatctgcgaa tcgatccgca gaccaaggag
     9241 ggggtcatgg cggaaatgga cggccccgga tgcattttcc ggatctggtc ggcgaacccg
     9301 caaggcgtca tccgtttcta cctggatgga gacgccaagc cgacgtacga gtgggatttc
     9361 agcaagcttt gcacgggtgt tattgcgccg ttcgtgcggc cgctggtctg gaaacgggat
     9421 cccaacgacg tgaattcggc ttcgaacatt tacctgccca ttccattcgc gaagagctgc
     9481 aaggtaacca gcgtgattcc gcagccggac ggttccggca agacgcccgg ccactactac
     9541 atcatcgact acaggacgtt tccgaaagac tggacggtgc cgacgttcaa gcttccgctg
     9601 accgagtcgc agcgggccgc ggttgaggag accgccttga agtggtcgag atgcggccag
     9661 acgggcgcgc atcctctcga tcgcttcgcg acgaagaaag aaacgatcgc gccgggccag
     9721 cgacttgtgg tcgcggagtt ggaagggccg gccgtcattc gacagtttcg ggcgaaggtg
     9781 aattcctccg agaaatgggc cacgcgaaag gtggggttgc ggatctattg ggacgacgag
     9841 acggagccgt cggtgtgttg cccgatcggc gattttttcg gcgagcccaa ggacgttgag
     9901 tacaagtcgt acccgatggg cattaccgac tcgatgaact actgcttttt tccgatgccc
     9961 ttccggtcga gggcaaagat cgatctggtc aacgaaggga agcagccggc gccggttgag
    10021 gtcgccgtcg cctaccggac gcaggaggtg ccggaagact gggcgtactt caaggccaag
    10081 ttccggtgcg aaatcgcctc ggagagcttc gactatccgc ttatcgagac cagcggaacg
    10141 ggtaagctcg tcggcatctg cttgttcccc gacaatattc acggcggatg gtggggtgag
    10201 ggcgacgaga aggtctatgt cgacggcgag aagttcccgt cgtggttcgg aaccggatcg
    10261 gaggattact tcggcgacgc gtgggggatt cgccatttca cgaatccctc tcatgggcat
    10321 cctcagaaga acgtggagcg aatgcagggt tgctaccgtt ggcacctcgg agacaacatc
    10381 ccctactaca agtcgctgcg gatgacgatc gagaactaca ccggtctgcc gcatgtgacc
    10441 agccgcaacg actacagttc ggtcgcctac tggtaccagt tgccgggtgg agaggatttc
    10501 tttgaggaag tgccgctggt ggaccgtatt ccgcgcggtt tcgtggccac gggagccttg
    10561 gagattgaac gttgtcagga tcccgcagcg ttgagcgatt cgcttcggct cgttgacgac
    10621 gcgggccttc ccaagcccct ctcgagcggc ggcggcctga tgttcgtggg caagctggga
    10681 gacgaagtaa ccttccaggt tcccgtcgcc gaagatgagc actatttgct tcgtcccgtg
    10741 ttggcccagg acgtgcgcaa ctccaggttt gagctgcttg tggacggcaa gccggccggt
    10801 gagcgcccct acctgcgcaa aggcgtgatg cccattacgc tcaagttcac cggcgagccc
    10861 gttgacggtg atcgttgtcg tgtgatcatc gactacatgc gtatggaggt ctaccgcaat
    10921 ttcatcaccc agtggcttgt cgtcgggcct ttcccgaacc ccgaacataa ggggttttcg
    10981 accgtctatc cgcccgagga gcgactcgac ttcggcgcca ggtatgccgg gaacgacaac
    11041 caggaactca cctggcagcc ggtcagcagt caggacggcg tcattatgct gcagggtaga
    11101 ttcaagccct ctgaagacgt cgtcgtctac ggggcctgca tcgtccgttc gccgaaggcc
    11161 gggaaacgca ccctgctggt gggcagcgac gacggcatca aaatctggtt gaacggcaag
    11221 ctcgtctggg agcacctggt gcgtcgggcc atcaagcctg acgaggaccg catcgaggtc
    11281 gaactcaagc agggagacaa cctgctgctg ctcaagctcg accaggggcc cggcgacatg
    11341 ggctgggcgg tgcggttcct tgatccggcg gatgagttgc agtttgcact accgaagtga
    11401 ttcccgggag cggcctttgg tttcgcttac ccacattcgt gaggacctgt gcttcgagca
    11461 cgactgatgc gctacctgct cgacaccggc atttttgaga attgtcaatc gccagcgccg
    11521 atgcccgatt gacgctttcg gtcgagacac ttacgatgtt tgccggccgt ggggcaccca
    11581 cgcggatggg ctgccctaag gtctttattc ggcgtatgtt atggtagcga tccgccgctg
    11641 ctgcggcgag ggccgcgagt tgatcccccg tttgatacaa ctggttcaat cattcggtcg
    11701 tcagcgggtc ttgctgatcg gtgatctcat tctggatcgc tacgtctacg gcgacgcgga
    11761 gcgaatcagc ccggaggcgc ccgtcgtcgt gctccgcaag gagcatgacg aagaacgggt
    11821 gggcggagcg ggcagtgtgg ctgccggcat gcgcgagctg gggttggagg tggttattgt
    11881 cggtgcggtg ggtctggatg acgccggtca tcggattcgg gctttgctgg aggctcaacg
    11941 gatcggaacg cggggtctga tcgccctgag cgaccgtccg acgacgacca agacgcgttt
    12001 catcggcctg gctcagcacc gccaccgcca gcagcttatc cgggtggacg acgaggttac
    12061 gcatccgctg tcggagccgg acgcggctcg agttacccag atggcggtcg cgggtgtcaa
    12121 ggacgttgct gctgtctgcc tggaggacta cgacaaaggc ttgctgagcg aatcgttgtg
    12181 tcaaaaggtc atcgccgtcg cgtcggccgc cggcaagccc gtgctcgtgg atccagcccg
    12241 ccttggcgac tacagcaagt atcgaggggc gacgttgctg acgccgaatc gggccgagtt
    12301 ccaattggcg tcgggctgcc gtgatacatc ggtggaaacc attctggtca gcgctcacgg
    12361 ggtgatcgag aagtacgacc tgggcggact tctggttacg gttgatcgcg acggagcgat
    12421 tctggcggaa aagggcagga aggcagtgca catcgcgacg cgacctcgcg cggtctacga
    12481 caataccggg gcgggcgatg ccgtgttggc catgctggcg gcggcaattg tcgccggagc
    12541 aacgtgggag gaggccgccc ggctaaccaa catcgccggc ggtctggagg tggagagatt
    12601 cggctgcgtt ccgatcacgc aggaggaggt cattgccgac ctgcgtctgt cagccggggc
    12661 gggaaccggc aagatacgca ctgccgatga actcgtcacg gagttgtctc tccggcgaag
    12721 ccgccacgaa acggtggtat tcactaacgg ctgttacgac attctgcatg ccggtcacgt
    12781 tcgtttcctg gaacagtgca aagctctcgg caatatcgta gtggtcggtc tcaactccga
    12841 tgcttccgtt cgcgctctga acaagggcaa ggaccgtccg atcgttcccg agcggcaccg
    12901 agccgaggtg atcgcggcat tgcagagcgt ggactacgtg gttctgttcg atgaaccgac
    12961 gccgctgcgg ctgatcgaga agatacgacc ggaggtgctc gtcaaagggg aagattggaa
    13021 ggacaaaggg gtcgtcgggc gtgagttcgt cgaatcgcaa ggcgggaaag tgattctgct
    13081 gccgctggtc gagggtttga gcacgacgag actgatcgag cggattcgcg cgggggcgta
    13141 tgacgaataa tcccgatgcg gccatcgctt ccgatcatgg ctgactgctt tatgggctgg
    13201 aggcccgcaa gaatctgcgg catgatatcg gccagtccgc gggaatcctg caaaccttgc
    13261 ccaaagggcg tcgggccggt accctatcgt ggatcatgaa gtcatcgtca tcagaagtgt
    13321 tgcttgacgc cgccggcatt gccgcggtac ttgagcgaat ggcccgggag atcttcgaca
    13381 gccttccgga aggcgtgccc gtggcgatca tcgggatcct gcggcgaggc gaggttctcg
    13441 cgcagcgtat acagaccgcc ctgcttcggt tgggcgctcg ggacattcgc cacggaacgc
    13501 tcgacatcac cctgtatcgc gacgacctgg cgagcatcgg ttctcaggcc cggctaaggc
    13561 aaacggctat cgacttcgac atcgagggtg tgtaccttat cttggtggat gacgtcctgt
    13621 acacggggcg gtcgattcga tcggccatgg acgccctggt ggacctggga cgccccaagg
    13681 cgattcgcgt cgcggttctg gtcgagcggc ccggccgcga gctgcccatt caacccgatt
    13741 tcgtgggtgt tcgtatcgga cgcgaggata tgcccgtcac ggtcagcctg gccgagtcgg
    13801 acggaatcga tgaggtgcgc gtcggatgaa aaccgacaca agaaccgcgg ctgcgccggc
    13861 aggggccggg ccggccccga agccggaacc ggagttcacg tgggcgcggc gacacctgct
    13921 ggggctggaa gacctgaatc gcgaggagat cctgcacatc ttccgcacgg ccgagagcct
    13981 gcaggacatc tcgacccgga gcattaagaa ggttccatcg cttcgtgggc gcgtcgtggt
    14041 caacttgttc ttcgagaact cgacgcgaac caagaccagt ttcacgctgg cagcccagcg
    14101 gctatcagcc gatgtcattg atttcaccgc cgcgagttcg tccgttgcca agggggagtc
    14161 gctccgcgac accgtacgaa acatcgaggc gatgggcgtc gacacattca tcatccggca
    14221 cacggcggcc ggggcccccc acctgatcac ccgttgcacg cagtgccacg tcatcaacgc
    14281 cggtgacggg cagcacgaac atccgacgca gggcctgatt gacattttca cgattcgcga
    14341 gcgaaagaag cggatcgaag ggctcaacgt ggtgatcgtg ggcgacatcg cccattcaag
    14401 ggtagccagg tcgaatttct ggggcttgac gcgactgggg gcgcacgtaa cgttctgcgg
    14461 tccgacgacc ctggtaccac aggcatttga aaagtttggt gcccgggtga cacataatct
    14521 cgatgaagcc atccgcgacg ccgacgtcat caacatgctg cgcatccagc acgagcggat
    14581 gaccagcagc gtgttcccca gcacgcagga gtacgtccgt cttttcggcc tgaccagcga
    14641 gcgcgtccgg cactgcaagc ccgacgtgct gatccttcac cccggcccga tcaaccgcgg
    14701 ggtcgagctg gccagcgacg tggcggacgg ccccaacagc tcgatcctcc gccaggtctc
    14761 caacgggctg gcgatccgga tggccgtcct gttcctgctt aaccagacgt ccgaggcagc
    14821 gaaatgatcg ccctcttggg gggcagaagc gtttgacagg caagaccggc cgcacctgtg
    14881 atgagcatgg gagcggtacc cttgcgtgag cccggttcga ctgcgacgga gcgaggaaaa
    14941 cctaaggcgg accacctttt tacgggccgc aacccaagcg ctctgcgatc cagaaacatg
    15001 cgcgcagaat gcacgccagt cgcgacggaa gagactaacc ggtgtggatg ccggcgatga
    15061 gggcctggag gaccggcaag cattcaacgg cagccatgca caacaagtcg tgtcgagatg
    15121 gcccgcaact gagtagcgag agaggtattc ggaatgaggc gcgtatttac tttcctcaca
    15181 tggggcggga ttgtcatagg cttgaccttg gccggagtga cctgcaatgc gggcaacgac
    15241 ggagcggata acggaggcgg cgatggaaat ggtggaggcg gtggcgatgg gccggcccac
    15301 cttgttgaag tccctgaccc actctccggg gacaatccgc ggtatccgct tgtgacaaag
    15361 agcgtgccgg ccgccggcgg ttccgtgacg gacgcggcct tcgggattgc ccaaacgcgc
    15421 gtcgtgcaga ccgagggttt gcggcatgag tactcacgtc tggacccgtt caacagcgac
    15481 cgtacgctga tcctgcttct gctcacatcg gccggcgagt ggcgcgtcta ccgcacccaa
    15541 agcgtacctt acgaccagag cggcaatctc gtccgcacgc ttgacctggc tgagcctcga
    15601 tgggacccca gcgacgcgaa cctcgtctgg gggcttcgcg atttccaaat cgttacggtc
    15661 aacgtccaga ccggagacga gaccgtggtg aaggacttca cgcaggacgc gacgattgcg
    15721 ccgatcctga cggctaatcc ggacctttac cggattacga tgaaggatga aggcgaatcc
    15781 tcgaccgaca atcgctattg ggcgttcatg ctccagggca cggccgacga ctaccggccg
    15841 cgatacctgc tgacctggga ccggcagcag gatctcgtgc tcggcacctg ggccattccc
    15901 ctgaacgaat cacgcattga ctgggtgggc atgtcgccgc tgggcaactg ggtggtgatc
    15961 ggtgcagatt gggacaacgg tgggggcaag tccggcatgg taatcgccga tcgggggttc
    16021 tcgcagttcc accagatcga ctacaacgcg ggccactcgg atgtcgggtt ggacagcgag
    16081 ggtaacgagg tcatcgttct ccagagcaac cggaccgatt ccatcgacat gctcccgctg
    16141 agcccgaaca ccgagccggt cgacaccggc gagagctatg caggcacggg acgcgtccag
    16201 gtcatccgcc tgttctacga ctcaagctcg cccctgggcc tgaacagcgg gatccacatc
    16261 tcctgcaatg cccccggctg gtgcgtggtg tcgacgttca ccgaaccggg cctgcccgag
    16321 cagaactggc tggaccgcac gatcaccctg gtcagactcg acccgagtca cccgcgcgtg
    16381 ttctatctgg ccaaggttca cggcacggcc ggggcctatt gggaggaaac gcaggccagc
    16441 atcacgcgcg acggctcgcg cgttgtctgg gccacaaact ggaaccggaa cgtcggccag
    16501 gagcgggtct ggctcataca attggaaatc ccggacgaag cgatggcttc gattggtggt
    16561 taaagatcat cgttttcact atctggagga tcccatgatg tggcgtcgcc tgaacaagct
    16621 tgggccgatg gtggtcatgc tggtttcact ctgtttcgga gcggtgtgca ctccgaacga
    16681 cgacgagaac ggcggcgagg tcgacccgcg cgactttgcg ctgccggtt
//
LOCUS       DAOTPW010000120        16704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3437_length_16704_cov_5.559013, whole genome shotgun sequence.
ACCESSION   DAOTPW010000120 DAOTPW010000000
VERSION     DAOTPW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16704
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_16704_cov_5.559013"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="P5316_12065"
     CDS             complement(<1..240)
                     /locus_tag="P5316_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85734.1"
                     /translation="MSYAIEPVVQDGNKCGEAPTWDAPNRRVLWTDNETDTTYQYNVD
                     TGERKVLTRGVVVSGIALNRSGGLLLGGPTGLHFWQ"
     gene            640..1461
                     /locus_tag="P5316_12070"
     CDS             640..1461
                     /locus_tag="P5316_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85735.1"
                     /translation="MPEGYDTHADYMKWVKAEAETRRAAIVAAVTEAFAPFIERREVD
                     VINFSAMTAMDLAAAIQAHPAILKALLACCNVAGRAIERDLDIRGIDTYEPHLTEAKA
                     AAIAGYLKSFLPQEACVPALCELDRFFFVDKEIRARKGRWERMILHALNGQSRLVFRK
                     RKFNVAGEFFELDAAAPESGPIEIAVDVKRIEARRDIHKRADEIINKAAKFKTAYPHG
                     RFAAVVYYPFTAEHVNVQSRLDSPNIDTVCFASQSAEQVSITIGLLVDRLGIRRR"
     gene            complement(1458..2432)
                     /locus_tag="P5316_12075"
     CDS             complement(1458..2432)
                     /locus_tag="P5316_12075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRR85736.1"
                     /translation="MGKSEPSLFADERSRASRTRADATRIPEPQVYEGPPTAHVLNLG
                     DARELDWIADQSVHLVVTSPPYYNLKKYNDHPAQLGNMEEYQAFHDELDKVWRHCYRV
                     LTPGGRLVCNVGDVCVARRANRGRHLVLPLHADIAVRARRIGFDYLTPILWHKIANAQ
                     FEAAGNGAGFLGKPYEPNAIIKNDVEYILMLRKPGQYRSPTEQQRATSRLTKEEQARW
                     FRPIWSDVTGASTREHPAPYPVELAYRLIRMFSFTGDTVLDPFAGTGTTTIAALMCNR
                     NSVANEIDLQYFAIAEKRIRGEIAQGRMFGGAPSLKVRQSLAAGHSPD"
     gene            2998..3732
                     /locus_tag="P5316_12080"
     CDS             2998..3732
                     /locus_tag="P5316_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85737.1"
                     /translation="MRRGFTLTELLVVIALIVIMSGLSAPAIRAYLLNREQTLAVRVF
                     SGAVSNAQALARAHFTTTAVRVERAFATDDSGRMLRNPAGRPYWLDHQKIQILAVGMH
                     QPQKPVAGEALVFRRLLDAPAVDLPSSAWLAPGNALSLLASGNTTYQPSAAGATAIDT
                     LDTFYLVFNRQGTLVRLPASRLVYLDESQDNAFIEHPQPSQMGAITYNRGRFEKSGRD
                     PTWLEKGIPLYINQRTGSVIGAHASR"
     gene            complement(3751..4797)
                     /locus_tag="P5316_12085"
     CDS             complement(3751..4797)
                     /locus_tag="P5316_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR85738.1"
                     /translation="MREPTPRELVLRTVRFERTPRVPRNLWTLPWAETHYPVELQTIR
                     RDFPDDIVSPTVDFPRPDCMRGDPYTPGVFVDEWGCEFLNIQAGAIGEVKNPPVRSYA
                     SDLDKVRPPWAWARIDLGLVAEQYAGTDRFMLSPLCFRLFERMQFLRGTEALYIDLME
                     QPAGLTEMTRRIHEWNLAMVDAWAATDVDAILGMDDWGSQRSLLISPRLWRDFFKPYY
                     RQYVERIHAAGKLCFFHSDGYIFDIYEDLIEIGVDAVNSQLFCMDIEEIGRRFKGRIT
                     FWGEVDRQHLLPYGSIEDIRGAVRRAAAALYGGQGGVIAQCEFGVGARPENVRAVFEE
                     WSRVSREVGQSFGQ"
     gene            4949..6448
                     /locus_tag="P5316_12090"
     CDS             4949..6448
                     /locus_tag="P5316_12090"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR85739.1"
                     /translation="MRSTAAVSVLILSIHSAVFPAGLDELATVTGHTSHRTSSADPTG
                     GNEDSLVTFVPGETRTLLDTDGPGRITHMWFTAARFANHETFFRDIVIRMYWEHSPVA
                     SVEVPLGDFFALGHARQYVISSAPVCVGDNTKALNCYWPMPFRKHARIELFNNGRRSI
                     RRVYFNIDYELGPQPPDQGLFHAEFRRAPALRTQAHERNLTGRDNYVILETEGRGQYV
                     GCVLAVDAQPGGWWGEGDEMIFLDHAERPTIAGTGSEDYFCNAWGFQKAFSNPYYGCP
                     LLEQLPDGGSLTTVYRWHIPDPVRFKTHIRVTIEHLFGPKVVNDYSSVAYWYQTEPIR
                     QREPLPAAEENHPRSHAIPATQPVTTIEMSATELEPGLHARGIKVRSVTTSLGAGYAS
                     GGYLEIDAPDQRVEIPVPVPADGRYRVRVKPVNTLIKDQLQISLKGGKTAAFVRHDGH
                     ERNTPHVDLGETRSSDGRLFITLSGNPVMGLDHLRIERTDMQDVPAAPR"
     gene            complement(6520..6903)
                     /gene="acpS"
                     /locus_tag="P5316_12095"
     CDS             complement(6520..6903)
                     /gene="acpS"
                     /locus_tag="P5316_12095"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRR85740.1"
                     /translation="MKIIAQGIDLIECKRIRDVWERHGDRLTDRLLTPAELAYVRANR
                     DPVPRLAGRFAAKEAILKVLGTGWKGKIAWRDMEILNDAAGRPYVTLCDECKQVADSL
                     GITRILISITHTENFAAAVATGVAD"
     gene            7581..9764
                     /locus_tag="P5316_12100"
     CDS             7581..9764
                     /locus_tag="P5316_12100"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRR85741.1"
                     /translation="MSVEHLLDGLTEPQKEAVTHAHGPLLVLAGPGSGKTRVITHRTA
                     YLARTVTEPRHILALTFTNKAANEMAERIRHLAVEPGVTCCTFHSLCARLLRTYADRA
                     GLNPNFSIFDQTDQTAAMKQAIVRCGMSAENYSPANMLEAVSQAKNDMVLPEDYAQYA
                     RDWSDRAIIPIYEAYQQILAEQNALDFDDLLLKTAYLLGEDAELRDQLQDRYRFVLVD
                     EYQDTNHAQYLIARGLALDHENLCVTGDPDQSIYGWRGANIHNILEFEEDFSKTKVIR
                     LEKNYRSTPQILAAADAVIRHNRNRKHKRLWTDNSGGPPVRVIEADTANQEADLVAEA
                     ISEHIAGGGKYADVAVFYRTNALSRLIEETLLKRHIPYQVARGVSFFQRKEIKDSLAY
                     LRLAANPKDRVSFLRIVNVPPRGIGDTTIERIVKKADETGLSPLDVMADPRGLKVSAH
                     TAERMTWFVKLIHEIAGIAADESVTAAVQHAILHSGLRAAWNQAKDDDAGRNADELIS
                     DAADYDRDHPQGASLVDWLQQISLMSEVDSVNPEMGAVTLMTLHAAKGLEFNRVFIIG
                     VEDGLLPYRRQENREADVEEERRLCFVGMTRARRSLTLTCARFRSIRGRGSRSARSVF
                     LTELPREQVEWLVLPSDRGTEVNRGYDDQAAEAMAVRYDDWQQGRLVRHEDYGLGQIL
                     WLRPAARGVCAGIRFKRDGSERTFVLEQAAAKLQLLEYDDSGWDS"
     gene            9761..11143
                     /locus_tag="P5316_12105"
     CDS             9761..11143
                     /locus_tag="P5316_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRR85742.1"
                     /translation="MTTESTLTLDTKHVTDERLGEHRLTEADLRSLAPRVAEILKEIA
                     AERGRGDHRFRELPHERPQVDEIKKIVAERRDAIENLIVLGIGGSALGNIALQSALNP
                     LTYNLLPKTERRGPRLFVLDNVDPAYFEAVMHFINEDGWANTLVNVISKSGETAETSA
                     QFLIVRDRLNKALGEAEAKEHILVTTDSSKGTMRKIVDAEGYRSLIVPDGVGGRFSVL
                     SPVGLLSAAFCGIDIDSLLDGADAMDKTVSNPDPINNPAALMAMIYYLYDQRGKNIHV
                     MMPYSQQLRDLADWFRQLYAESMGKIRPSDGKCVGPTPIKALGVTDQHSQVQLYREGP
                     NDKVFTFLEVQAFECDSIIPLAFEGIEAMEYLGRSKNGTLAGLLAAEKRATELALLVS
                     KRPSLTVRFPEVKAHTVGEFIYMYEAMIPIMGKLYDINPYDQPAVELGKVLTFHFMGR
                     RGYEKPAIEL"
     gene            11259..12329
                     /locus_tag="P5316_12110"
     CDS             11259..12329
                     /locus_tag="P5316_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRR85743.1"
                     /translation="MHHAVIMAGGSGTRLWPLSRRSHPKQLLRIFEGKSLLRRSFDRL
                     RALLPAEQIHIITGRDYIEPMAAELPELPRENLMGEPCPRDTANAVGLAAHLLALRDP
                     DGTMGIFTADHIIHPIDTFAATVRQGFEAADRHADALITFGITPRNPHTGYGYIHRGP
                     QVDPGVFEVLEFKEKPDLETARRYVESGEYYWNSGMFVWRLPAIIAQIRKHQPQIDAG
                     LREVAAGFHDPARADAILARFSSLPKISIDFAVMEKADRVLTVEMPCEWLDVGSWPSL
                     AEVFKPDEAGNILAAPNVMTLDARDNILVSESDHLFAAIGVSDLIVIHSDDATLICRK
                     QDAQRIKDLVQQARQTTGERYM"
     gene            complement(12408..15245)
                     /locus_tag="P5316_12115"
     CDS             complement(12408..15245)
                     /locus_tag="P5316_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HRR85744.1"
                     /translation="MAALVFLCGCGGGVAIGSGSVQVWPVPASAIVFSDAVPTGYSEL
                     YRETEKTIRIDAAINEVASFQLAMAAQGNVPAGVRNIRVTDFRQGAVSVAASHVRLYR
                     ELRIPVEDYPAWYLRLTPHLRAKREYPDVLVPLESPKGALPIDLPPGRTEAVWVDVHI
                     PPGTEPGAYSGRIELETGGGATQGLAVLLTVWPFAIPQTRHAPVIAGLDTAGLIRNHV
                     EIDRRPYSPSRLSSEDPFYERAVQVVDQAMRLLHEHRLSPVLWDLRPAQRVVSGGGVE
                     LDWSDYDRLVSGIMDGSAFEDRTAAAAWPLPFDERDPSPDLHGGVGSETYERYVADYL
                     SKCVAHFERRGWLDRHFVWVPLSDGTRAERFKAFERIGRLLKRVEPRLRLVCPLSPMS
                     LEPYGYLNDPFVDVSNLVSIWCPPSAEADRTVLARERTVGKQVWLSPGRPPYTGSLSV
                     IASPVDAGSLAWQIYRFGYDGACLSVVNDWGREDAIGPTGSERQLIWSGKPYGLGGPV
                     PSIRLKRLLRGLQDYEYLWLLDRNRRPAIAKLLSTDLFRYGGTECFAEHFLDSRGPGW
                     VTEPAAWSIARGVMARELQVALSEQGDRPQSGQQDDVARFAEEMEWSRLVVAIRRTQV
                     VIEGVKARFEPQDAAGPVHLEATVSFFNATPRPFEGRLLWAGLPQGWTADDDGRPIGP
                     IEPTRGARVVIGAKARVIQPNTDGIVPVQFVLQPSQGEPTVFTGRLCVLVAQRIGKPF
                     VVDGRLDDWPLATNVASDFLLVGAGEVPKTGRASPNRPSQLTTVFACSDDEALYIAFN
                     CAESDLAGRRITRSNYVEYDDLWPVGEDLVEMVLDPSGQAVGPGDLYHVVVKANGAVL
                     AEKGIRCLDRVAPVSSWASGITAAVDDGLRPGCWTVEIRIPLASLGKRGDVIGANFAR
                     FDARRGEYSSWTACRSHIYSPITLGNMQLGR"
     gene            15750..16178
                     /locus_tag="P5316_12120"
     CDS             15750..16178
                     /locus_tag="P5316_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HRR85745.1"
                     /translation="MVTQLPDGTVEFRFYRPGARHVTLAGDFNGWNTTSLAMQMDKDG
                     WWRYVIHLTPGCYQFRYVADGEWHTDYAAFGLEHGPFGVNSVVKVEPAPASRTATARP
                     PVLRFVNSREADEIMLAPGAGRQLDWSVDETEEELAEAFA"
     gene            16332..16415
                     /locus_tag="P5316_12125"
     tRNA            16332..16415
                     /locus_tag="P5316_12125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16366..16368,aa:Leu,seq:gag)
BASE COUNT         3420 a         5462 c         4877 g         2945 t
ORIGIN      
        1 ctgccagaag tgcaagccgg tcggtccgcc caacaggagt ccgcccgagc ggttcagggc
       61 aatgcccgag accaccacgc cgcgggtcaa caccttccgc tcaccggtgt ccacgttgta
      121 ctggtacgtg gtgtccgtct cattgtcggt ccagagcacg cgacgattgg gtgcgtccca
      181 cgtcggcgcc tcaccgcact tgttgccgtc ctgaaccacc ggttcgatcg cgtacgacat
      241 ctgagtctac ctccggcaga gtaccgagaa aacacgggat tcacgtcccg ccttctgaaa
      301 tgaaagcata acccaagacg gggcacgagt catccagaca ccgctctgaa acggttgaga
      361 tgtaagtaac gagaaatgcc gcatgcccac cggcgggatg atccgtctcg gcatcgttga
      421 cgaacgactg accttcgtgg tattccttgg ccaagcgata tcacacccac acacggaggt
      481 ctgcgggaga aagaccccgg agctgagaaa caaggctccg ttgattcctc gtgcgacgcg
      541 agccggtcgg atggtgtcgc gataccggcg tgcgtgtgag acgatacgcg gaaagctgtt
      601 catcttgttg aacggtgcgt ccgctgagcg gagatccgga tgcccgaagg atacgatacg
      661 cacgcagatt acatgaaatg ggtcaaagcc gaggctgaaa cgcggcgtgc ggcgatcgtc
      721 gccgccgtca ccgaggcgtt cgcacccttc atcgagagac gcgaggtcga cgtcatcaac
      781 ttctccgcga tgacggcgat ggaccttgcc gccgccatcc aggcccaccc ggccatcctc
      841 aaagccctgc tcgcttgctg caacgtcgcg ggacgagcga tcgagcggga cctggacatc
      901 cgcgggatcg acacctacga gccgcacctg accgaggcga aggccgccgc catcgcgggt
      961 tatctcaagt cctttctgcc gcaggaagca tgcgttcctg ccctctgtga gcttgatcgt
     1021 ttcttcttcg tggataagga gatccgggct cgcaagggcc gttgggaacg gatgatcctc
     1081 cacgccctga acgggcaatc ccgcttggtc ttcaggaaac gaaaattcaa tgttgcgggg
     1141 gagttttttg aacttgatgc cgccgcgcct gagagcgggc caattgagat cgccgttgac
     1201 gtcaagcgca tcgaagcaag gcgagacatc cacaaacggg ccgacgagat catcaacaag
     1261 gccgcgaaat tcaagacggc gtacccacac ggcaggttcg ccgccgtagt gtactacccc
     1321 ttcacagccg agcacgtgaa tgttcagagc cgtttggact cgcctaacat cgatacggtc
     1381 tgttttgcgt cgcaatccgc cgagcaggtt tctatcacaa tcggactact cgtggatcgg
     1441 cttggaatcc gcagacgcta atcgggactg tgtccggcgg ccagtgattg ccgcaccttc
     1501 aacgacggcg cgcctccgaa catccgccct tgcgcaatct cgccgcgaat gcgcttctcg
     1561 gcaatcgcga aatactgcag gtcaatctcg ttggccacgc tgttgcggtt gcacatgagc
     1621 gcggcgatcg tggtcgtccc ggttcccgcg aacgggtcca gaaccgtgtc tccagtaaag
     1681 gagaacatgc ggatgagccg ataggcaagt tcgacgggat acggcgcagg atgctcgcgt
     1741 gtcgaggccc cggtgacgtc actccagatt gggcgaaacc agcgagcctg ctcttccttg
     1801 gtcagccgcg acgtggcgcg ctgctgctcg gtagggctac ggtattgtcc aggcttgcgc
     1861 aacatgagaa tgtattcgac atcattcttg atgatggcgt tgggctcata tggttttccc
     1921 aggaagcccg cgccatttcc ggcggcctcg aactgggcgt tggcgatctt gtgccagaga
     1981 atcggcgtca ggtagtcaaa cccgatccgc cgggctcgca cggcgatgtc ggcatgcaac
     2041 ggaagcacaa ggtgtcgacc gcgattggct cgccgagcaa cgcacacatc ccccacattg
     2101 cagaccaggc gtcccccagg ggtgagcacc ctatagcagt gccgccacac cttgtccaac
     2161 tcgtcatgaa aggcctggta ttcctccatg ttgccgagct gagccggatg atcgttgtac
     2221 ttcttgagat tgtaataagg cggcgaggta acaaccaagt gcacagattg gtccgcaatc
     2281 cagtccagtt ccctggcatc tccgagattg agcacatgcg cagtcggcgg tccttcgtag
     2341 acctgcggtt ccggaatccg cgtggcgtcc gctcttgtcc gcgacgcgcg ggagcgctca
     2401 tcggcaaaaa ggctcggctc ggatttaccc atagcagcgg atattataca cgtgcgggcc
     2461 ccggcgaaga agaactgccc tccaggcaaa gtcgaggaca atcccagacg cgttcggccg
     2521 gcgacaggat tctgaaaggg gccgccgcaa agccccgcgt gaccggcccc gactcgtcag
     2581 cggctgacca cctgaatctg cgagtctgtc gcaccgagtc tcgtgccttt ccccctttgc
     2641 tttgtgtccg ttcgagctcg aaagcctgat gacggctatg ggggtcgacc cggcacgtcc
     2701 gatcatccgc ggccggacgc ccgccctatt gacgtaatat ccgggcaggg cgagcaggcg
     2761 tttcccccag tacgccgccg accactcagg tcggcacaac cggcggaatc gccgctatgg
     2821 acactgtttg gattcgtata ctttattggg acatgataga ggccttccgt tgagatcctc
     2881 gacaatgggc gaggttcggc cggaggctgg gtgctccgtt tcgggagacg aggcacgcgc
     2941 ccccgaccag tgtggggttt ggggaagcaa ttgcgcccga agggaggcgg ccgtgcgatg
     3001 agacggggct tcacgctcac cgaactgctg gtggtgatcg cgctgatcgt gatcatgtcc
     3061 ggcctttccg ctcccgcgat ccgcgcatac ctgctgaatc gcgagcaaac gctggctgtt
     3121 cgggtcttca gcggcgcggt cagcaacgcc caagccttgg cgagggcaca cttcaccacc
     3181 acggcggtcc gcgtcgagcg ggcttttgcc accgatgatt cagggcgaat gctccgaaac
     3241 cccgccggcc gaccctactg gctcgatcat cagaaaatcc agattctggc ggtcggcatg
     3301 caccagcccc agaaaccggt cgccggagaa gcgcttgtct ttcgccgtct gctcgacgcg
     3361 ccggccgtcg atttgccttc gtcggcttgg ctggctccgg gcaacgctct cagcctgctc
     3421 gcctcgggca acacgaccta tcagccgtcc gcagcgggcg cgacggccat tgacaccctg
     3481 gacaccttct acctggtctt caatcgacaa ggaacgctcg tgcggttgcc cgcaagccgg
     3541 cttgtgtacc tcgatgagag ccaagataac gccttcatcg aacacccgca gccgtctcag
     3601 atgggagcca ttacctacaa ccgcgggcgg ttcgaaaagt ccggccgcga tccgacctgg
     3661 ctggaaaagg gcattccgtt gtacatcaac cagcgaacag ggtcggtcat cggcgctcat
     3721 gcttcgcgtt gagcgacttg cacccggccg ttattgtccg aacgactgtc cgacctcgcg
     3781 ggaaacccgg ctccactcct caaaaacggc ccgaacgttc tccggcctgg ccccgacgcc
     3841 gaactcacac tgggcgatga cgcccccctg gccgccgtac aaggcggccg cggctctgcg
     3901 cacagccccg cggatgtcct cgatggaacc atagggcagc agatgctggc gatcgacctc
     3961 gccccaaaag gtgattcgac ccttaaaccg ccggccaatt tcctcgatgt ccatgcagaa
     4021 aagctgcgaa ttcacggcat cgacgccgat ctcgatcaag tcctcgtaga tgtcgaagat
     4081 gtacccgtcg ctgtgaaaga aacacagttt gccggccgca tggattcgct cgacgtactg
     4141 gcggtagtac ggtttgaaaa agtcccgcca caaacgcggg ctgatcagca gcgatcgctg
     4201 cgagccccag tcgtccatgc ccagaatcgc gtcgacgtcg gtcgcggccc aggcgtcgac
     4261 catcgccagg ttccactcgt ggattcgcct cgtcatctcg gtcaggcccg cgggctgctc
     4321 catcaggtcg atatagagcg cttctgtgcc gcggaggaat tgcatccgct caaagagccg
     4381 aaagcacagc ggcgaaagca tgaaccggtc ggttccggca tactgctccg ccacgaggcc
     4441 aaggtctatc ctcgcccatg cccacggagg ccgaaccttg tcgagatcgc ttgcatagct
     4501 cctgaccggc ggattcttca cctcaccgat cgcgccggcc tggatgttca ggaattcaca
     4561 gccccattca tcgacgaaga caccgggggt atacggatcg ccgcgcatgc agtccggccg
     4621 gggaaagtcc accgtcggcg acacgatgtc atccggaaaa tcgcgacgaa tggtttgcag
     4681 ttcgaccgga tagtgcgtct ccgcccacgg aagcgtccag aggttccgcg gcaccctggg
     4741 cgtccgctcg aaacgaaccg tccgcaatac cagttctcgt ggagtcggct ccctcattgc
     4801 cggactcata cacgatcggc aggccgcaat caacgtgaca tcctgaggcc acgacacgag
     4861 cggcgtggcc atgaagagca agtcggcgta ggctatcgtg acgttgccct ggagaatgcc
     4921 aaagcatgat gaccttcgga gacatccaat gcgatcgact gctgccgtgt ccgttcttat
     4981 cctgtcaatt cattctgccg tgtttcccgc cggccttgac gagttggcca cggtgaccgg
     5041 ccacacctca caccgcacat ccagtgccga cccgaccggt ggcaacgaag actcgctggt
     5101 caccttcgta cccggcgaga cacgaacact gctcgacacc gatggccccg gccggatcac
     5161 gcacatgtgg ttcaccgccg cccggttcgc caaccacgag acgtttttcc gcgacatcgt
     5221 catccggatg tattgggagc attcgccggt agcgtcggtc gaggtaccgc tcggcgactt
     5281 cttcgccctc ggccacgccc ggcagtacgt catcagctcg gcccccgttt gcgtcggcga
     5341 caacacaaag gctctgaact gctactggcc gatgccgttc cggaagcacg ccagaatcga
     5401 gctgttcaac aacggtcggc gatccattcg ccgcgtctat ttcaacatcg actacgaact
     5461 ggggccacag ccgccggatc aagggctttt tcatgcggag ttccgccgcg ctccggctct
     5521 gcgcacgcag gcccacgagc gaaacctcac cggcagggac aactacgtca tcctggaaac
     5581 cgagggccgc ggccaatacg tcggctgcgt tctggccgtc gacgctcagc ccggcggctg
     5641 gtggggagag ggcgacgaga tgatcttcct cgatcatgcc gagcgaccca ccatcgccgg
     5701 caccggctcg gaggattact tctgcaatgc atggggcttc caaaaggcct tcagcaatcc
     5761 ttactacggc tgccccctgc tcgaacaact gcccgacggc ggcagcctga cgaccgtcta
     5821 tcgctggcac attcctgacc ccgtccgctt caagacccat atccgggtga ccattgagca
     5881 cctctttggc cccaaggtgg tcaacgatta ctcaagcgtc gcgtactggt atcagaccga
     5941 gccgatcagg cagcgagaac cactaccggc cgccgaggaa aaccaccctc gatcccacgc
     6001 cataccggcg acccagcccg tcacaacaat cgagatgtcc gccaccgagc tcgaaccggg
     6061 cctgcacgcc cgcggaatca aggtacgcag cgttaccacc agcttgggtg ccgggtacgc
     6121 aagcggcggt tacctggaga tcgacgcgcc ggatcagcgc gtcgaaatcc ctgtgcccgt
     6181 ccccgccgac gggaggtacc gagtccgcgt gaagcctgtg aacacgctga tcaaggacca
     6241 gctgcaaatc agcctcaagg gcggcaagac cgctgcgttc gtcaggcatg atggccacga
     6301 gcgcaacacg ccgcatgtcg acttgggtga aacccggtct tcggacggcc gcctgtttat
     6361 caccctcagc ggcaacccgg tgatggggct cgatcacctg agaatcgagc ggacagatat
     6421 gcaggatgtg cctgcggcgc cacgttgact cgcctccccg ggggcttgac ctgcccctgg
     6481 ggctcgtcaa ggtatctgcc ggcggctgag ggctgaaggt cagtctgcaa ctcccgtcgc
     6541 cacggcggcg gcaaagttct cggtgtgcgt gatggagatg agaatgcgcg tgatgccgag
     6601 cgaatcggcc acctgcttgc actcgtcgca gagggtaaca tatggccggc cggcggcgtc
     6661 gttgagaatc tccatgtcgc gccaggcgat cttgcccttc cagccggtac ccagaacctt
     6721 gaggatggcc tccttggccg cgaaacggcc ggccaagcgc ggtaccgggt cgcggttggc
     6781 gcgcacgtag gccagctccg caggggtcaa caggcggtcg gtcagccggt cgccgtgacg
     6841 ctcccagacg tcgcggatcc gcttgcactc gatcagatca ataccctggg caatgatctt
     6901 catagggtga cagagtaacc ggacgccgcg gccatggcaa agaccacgac tgcggcgggt
     6961 ggtcagtagt cagtcttcag ttgtcagtac tctggcgccc ggcttccacg aagtggacac
     7021 ccgctggtgc gagtatcctt tctcgcaccc tccccgaacg aatcctttcg cccctctggt
     7081 cgagccgaac cgctggtgcg agaatccctt ctcgcacccc tcctccgggc gaatcctttc
     7141 gcccctctgg tcgagcagga taggaacccc gccgggggcc gagatgggaa tctcgaccca
     7201 gcgacagtcc gctggtgcga gaatcccttc tcgcacccct cctccgggcg aatcctttcg
     7261 cccctctggt cgagcaggat aggaaccccg ccgggggccg agatgggaat ctcgacccag
     7321 cgacagtccg ctggtgcgag aatcccttct cgcacccctc ctccgggcga atcctttcgc
     7381 ccctctggtc gagcgggata ggaaccccgc cggggtgaag ccgatatgag aatctcgacc
     7441 cagcgacagt atggggctca gatgggaatc tcgacccagc atgcacacca aacacgtacc
     7501 ggttccggaa ctgacaactg aacactgaca actcgccttc cggagtggta cgcgatataa
     7561 aaccacgtgc gaggatcgag atgagcgtgg agcacctact cgacggcctt accgaaccgc
     7621 agaaggaagc ggtgacgcat gcgcacggcc cgctgctggt gctggccggg ccgggcagcg
     7681 gcaagacgcg ggtcatcacc caccggaccg cctacctcgc ccggaccgtc accgagcccc
     7741 gccacatcct ggccctgact ttcaccaaca aggcggccaa cgagatggcc gagcgaatca
     7801 gacacctggc cgtcgagccg ggcgtcacct gctgtacctt tcactcgttg tgtgcccgac
     7861 tgctgcgaac ctacgccgac cgcgccggac tgaacccgaa cttctctatc ttcgaccaaa
     7921 ccgaccagac ggcggccatg aaacaggcca tcgtgcgttg cggcatgtca gccgagaact
     7981 actcgcccgc caacatgctc gaagcggtca gccaggccaa gaacgacatg gtcctgcccg
     8041 aagactacgc gcaatatgcc cgtgactggt cggaccgagc gatcataccg atctacgaag
     8101 cctatcagca gattctggcc gagcagaatg cactcgattt cgacgacctg ctgctcaaga
     8161 cggcctatct gctgggcgag gacgccgagc ttcgcgacca gcttcaggac cgttaccgct
     8221 tcgtgctggt ggatgagtat caggacacca atcacgccca gtacttgatc gctcgcgggc
     8281 tcgccctgga tcacgagaac ctgtgtgtga cgggcgatcc cgaccagtcc atctacggct
     8341 ggaggggcgc gaacatccac aacatcctgg agttcgagga ggacttttca aaaaccaagg
     8401 tcatccgact ggaaaaaaac taccgctcaa cgccgcagat cctggccgcc gccgacgcgg
     8461 tgattcgcca caaccgcaat cgcaagcaca agagactctg gaccgacaac agcggcggac
     8521 cacccgtccg agtgattgag gccgacaccg ccaatcagga ggccgacctt gtcgccgagg
     8581 ccatcagtga gcatatcgca ggcggcggca agtacgccga cgtggcggtg ttctatcgaa
     8641 ccaacgctct gtcacgattg atcgaggaga ccttgctgaa gcggcacatc ccctaccagg
     8701 tggcccgtgg tgtgtcattc ttccagcgca aggagattaa ggactcgctg gcttacctgc
     8761 gcctggccgc taacccgaaa gatcgggttt cctttctgcg aatcgtgaac gtgccgcccc
     8821 gaggcatcgg cgatacgaca attgaacgca tcgtcaagaa ggccgatgaa acggggcttt
     8881 ctccactgga cgtgatggcc gacccgcgcg ggctcaaggt ttccgcccac accgcggagc
     8941 gcatgacatg gttcgtaaag ctgatccatg agatcgccgg aatcgccgcc gacgagagtg
     9001 tcacggcggc cgttcagcac gccattctgc acagcgggct gcgggcggcg tggaaccagg
     9061 ccaaggacga tgacgccggc cgcaatgcgg acgagctcat ctctgacgcc gcggactacg
     9121 accgcgatca cccccaggga gcatcgctgg tcgactggct tcagcagatc agcttgatga
     9181 gcgaggtcga ctccgtcaac ccggaaatgg gagccgtgac cctgatgacg ctccacgcgg
     9241 ccaaggggct ggaattcaac cgggtgttca tcatcggagt cgaagacggt ctgctgcctt
     9301 accgtcgcca ggagaaccgc gaggcggacg tggaggaaga acgacgactc tgcttcgtgg
     9361 gcatgacgcg ggcccgccgg tcgctgacgt tgacgtgcgc gcgcttccgg agtatccgag
     9421 gccgcggaag ccgaagtgcc cgctccgtct tcctgaccga attgccacgc gagcaggtcg
     9481 agtggttggt tctcccctcc gaccgtggca cggaagtgaa tcgcggttat gatgaccaag
     9541 ccgccgaggc gatggccgtc cgatacgatg actggcaaca gggccggctc gttcgacatg
     9601 aggactacgg cctggggcag atcctatggc tccgccccgc cgccagaggc gtgtgcgcag
     9661 gcatcagatt taagagagat ggcagcgagc ggacctttgt cctcgaacaa gccgccgcga
     9721 agttgcagtt gttggaatac gacgattcgg gatgggactc atgacaacag aatccacgct
     9781 cacactggac acgaagcacg tgacggacga acgcctcggc gagcacaggc ttaccgaggc
     9841 cgacctgcgg agcctggctc cccgggtggc cgagatcctc aaggaaatcg ccgccgagcg
     9901 aggccggggc gaccatcgct tccgggaact gccccatgag cgaccgcagg tggacgagat
     9961 caagaaaatc gtcgccgaac gccgcgacgc aatcgagaac ctcatcgtac tgggtatcgg
    10021 cggttcggcg ctgggcaaca tcgctcttca aagcgccctg aacccgctga cctataacct
    10081 gctgcccaag accgagcgcc gcggaccgcg attgttcgtg ctcgacaacg tcgacccggc
    10141 atatttcgag gccgtgatgc acttcatcaa tgaagacggc tgggccaata ccctggttaa
    10201 cgtcatcagc aagtccggcg agacggccga gacctcggcc cagtttctca tcgtccgcga
    10261 ccggctcaac aaagccctgg gcgaggccga agccaaagaa catatcctcg tgaccaccga
    10321 ttcgtccaag ggcacgatgc gcaagatcgt ggacgccgag ggatatcgtt cactcatcgt
    10381 gccggacggc gtcggcgggc ggttcagcgt gctctcgccg gtcggtcttc tctcggcggc
    10441 tttctgcggc attgatatcg attccttgct cgacggggcg gacgccatgg acaagaccgt
    10501 cagcaacccc gacccgatca acaaccccgc cgcgttgatg gccatgatct actacctgta
    10561 cgaccagcgc ggcaagaaca tccacgtgat gatgccctac agccagcagc ttcgcgacct
    10621 ggccgactgg ttccgccagc tctacgccga aagcatgggc aagatccgcc cgagtgacgg
    10681 caaatgcgta gggccgacgc cgatcaaggc attgggcgtc accgaccagc actcgcaggt
    10741 tcagctttac cgtgaaggcc ccaacgacaa ggtcttcact ttcctcgagg tccaggcctt
    10801 cgagtgcgat tcgatcatcc cgctggcgtt cgagggtatc gaggcgatgg aatacctcgg
    10861 ccgcagcaag aacggcacgc tggccgggct gctcgccgcc gagaagcggg caaccgagct
    10921 ggccctgctg gtcagcaagc ggccgtcgct gaccgtgcgt ttcccggagg tgaaagccca
    10981 taccgtcgga gagttcatct acatgtacga ggcgatgatc ccgatcatgg gcaagctgta
    11041 tgacatcaac ccatacgacc aaccggcagt ggagctcggc aaagtgctga ccttccactt
    11101 catgggccga cgcggatatg agaagccggc aattgagttg tgagtgatcg gttatcagtg
    11161 atcagttatc agtgaaagat gcagacagaa cccagacagc tctgactgag aactgacaac
    11221 tgaaaactga caactgagaa ctcccgcgga gttctcccat gcaccacgca gtcatcatgg
    11281 caggcggttc cggcactcgg ctctggccgt tgagtcggcg cagccatccc aagcagttgc
    11341 tccggatctt tgagggcaag tcgctgctac gccgatcctt cgatcggctc agggccctgc
    11401 tgcccgccga gcaaatccat atcatcaccg ggcgcgacta catcgaaccg atggcggccg
    11461 agttgcccga actgccccgc gagaacctta tgggtgagcc gtgcccgcgc gacaccgcca
    11521 acgccgtcgg cctggccgct catctgctgg ccctgcggga ccccgacggc acgatgggca
    11581 tcttcacagc cgatcacatc attcacccga tcgacacctt cgcggccacg gtgcggcaag
    11641 gcttcgaggc ggccgaccgg cacgcggacg cgctcatcac tttcggaatc accccgcgca
    11701 acccgcacac cggttacggc tatatccatc gcggcccgca ggtcgatccg ggggtcttcg
    11761 aggtcctcga attcaaggag aagcccgatc tggagaccgc caggcgatac gtcgaatccg
    11821 gagagtacta ctggaacagc ggcatgtttg tctggcgcct gccggccatt atcgcccaga
    11881 tccgcaagca ccagcctcag atcgacgccg gcctccgcga agtcgccgcc ggcttccacg
    11941 atccggcccg agccgacgcc atcctggcac gcttctcatc cctgccgaag atctcgatcg
    12001 acttcgcggt catggaaaag gccgaccgcg tgctgaccgt ggagatgccc tgcgagtggc
    12061 tcgacgtcgg ctcatggcca tcgctggccg aggtcttcaa acccgacgag gccggcaaca
    12121 tcctcgccgc cccaaacgtc atgactcttg acgcccgcga caacatcctg gtcagcgaat
    12181 cggatcatct cttcgccgca atcggcgtga gcgatctgat cgtcatccat tccgacgacg
    12241 caaccctcat ctgccgcaag caggatgcgc agcgaatcaa ggatctggtt caacaggcgc
    12301 gacaaaccac gggcgagcgt tacatgtgag ggttcagggt tcaggccggt ggaatccgcg
    12361 ctcacggacc gtcttgctga atgctgatcg ctgatagctg acagctatca ccgccccaac
    12421 tgcatattcc ccagggtgat cgggctgtag atatgcgagc gacaggcggt ccaggaagag
    12481 tactcgccac ggcgggcatc gaaacgggcg aagttcgcgc cgatcacgtc gccgcgcttt
    12541 ccaagagagg ccaggggaat ccggatctcc accgtccagc agccgggccg aagcccatcg
    12601 tcaaccgcgg ccgtgatccc cgaggcccac gatgaaaccg gtgcaacccg atcgaggcac
    12661 cgaatcccct tctcagcgag caccgcgccg ttggctttca cgacaacatg atacagatca
    12721 cccggcccga cagcctgccc gctcggatcc aacaccatct ccaccagatc ctcccccacc
    12781 ggccatagat cgtcatattc aacatagttg ctgcgggtga ttcgccggcc cgccaaatca
    12841 ctttcggcac agttgaaggc gatgtacaaa gcctcgtcgt cgctgcatgc aaaaaccgtc
    12901 gtgagttggg acggccggtt cgggctcgca cgccccgtct tgggcacctc gccagcaccg
    12961 accagcagga aatcgctggc cacgttggtc gccaacggcc agtcgtccag ccggccgtcc
    13021 accacaaacg gcttgccgat cctctgagcc acgagcacgc acaagcgccc tgtgaacacc
    13081 gtcggctcgc cctgggaggg ctgcagaacg aactgcaccg gcacgatgcc gtcggtgttt
    13141 ggttggatga cccgggcctt ggccccgata accaccctcg ccccacgagt cggttcaatc
    13201 ggcccgattg gtcggccatc atcgtcggcc gtccagccct gcggcagccc cgcccacagc
    13261 agcctgccct caaaaggtct cggcgtcgcg ttgaaaaacg acactgtcgc ttccagatga
    13321 accgggcccg ctgcgtcttg cggttcgaat cgggccttga cgccttcaat cacgacctgc
    13381 gtgcgccgga tcgccacaac cagacgcgac cattccatct cctccgcaaa tcgggccaca
    13441 tcgtcctgct gccccgattg aggacgatcg ccctgttcac taagagcaac ctgcaactcc
    13501 ctggccatca ccccccgagc gatcgaccag gccgccggct cggtcaccca accggggcct
    13561 cgactatcga gaaaatgctc agcgaaacat tccgttccac cgtagcgaaa caggtcggtc
    13621 gacaacaact tggcgatggc agggcgcctg ttgcgatcca ggagccagag gtattcataa
    13681 tcctgaaggc cccgcagcaa ccgtttcagc cggatcgacg gcaccggccc gcccaagcca
    13741 tacggtttgc ccgaccagat gagctgcctc tcgctgccag tcggaccaat ggcgtcttcc
    13801 cggccccagt cattcacgac ggacagacac gccccgtcat agccgaagcg gtagatctgc
    13861 caagccaggc tcccggcatc gaccggcgaa gcgattactg acagcgaacc cgtgtacggc
    13921 ggacgaccgg gactcagcca gacctgcttg cccacggttc gctcgcgcgc caaaacggta
    13981 cgatctgctt cggcagaagg cggacaccag atgctcacca ggtttgagac gtcaacaaac
    14041 ggatcgttga gataaccgta cggctccagc gacatcgggc tgaggggaca gacgagtcgt
    14101 agccgaggct cgacccgctt caacagcctg ccgatccgtt caaaagcttt gaaacgctcg
    14161 gccctggtgc catcgctcaa tggcacccag acgaaatggc gatccagcca tccccgccgt
    14221 tcaaaatgcg ctacgcactt gctcaggtaa tcggccacgt atctttcgta cgtttctgat
    14281 cccacaccgc cgtgcaagtc gggcgacgga tcgcgctcat caaaaggcaa cggccacgcc
    14341 gccgcggccg ttcgatcctc gaatgccgac ccgtccatga ttccgctgac cagcctgtcg
    14401 taatcggacc agtccagttc gactccgcca cccgaaacga ctctctgtgc cggcctcaga
    14461 tcccacaaga ccggactcaa ccggtgctcg tgcaaaagcc tcatggcttg gtcaaccacc
    14521 tgaaccgccc gttcgtagaa tggatcttca ctggacaaac ggctgggaga ataaggccgt
    14581 ctgtcaatct ccacgtggtt cctgatcagg ccggccgtat caagaccagc gatcacgggg
    14641 gcgtgccggg tttgcgggat ggcaaacggc caaacggtca gcaaaacagc caaaccctgg
    14701 gtcgcgcccc caccggtctc aagctcgatt ctgcccgaat acgcaccagg ttccgtcccc
    14761 gggggtatgt gtacatcaac ccataccgcc tccgtccgtc cgggcggcaa atcgatcggc
    14821 agggcaccct tgggagactc cagcggcacg agcacatccg gatactcacg cttcgctctc
    14881 aggtgcggcg tcaaccgcaa gtaccaagcc ggatagtcct ccacaggaat ccgtaattcg
    14941 cggtacaacc tcacatggct cgccgcgaca gatactgcgc cctgccggaa atccgtcacg
    15001 cggatgttcc gcacgcctgc aggtacgttg ccctgtgccg ccatggccaa ctgaaacgag
    15061 gccacctcgt tgatcgctgc atcgatccgg atggtcttct cggtttcgcg gtatagctcg
    15121 gaatagccgg tgggaacggc gtccgaaaag acgatggcgg acgcaggaac aggccagacc
    15181 tgcacactgc cggaaccgat ggccacgccc ccaccgcagc cgcagagaaa aaccaaggcc
    15241 gccacggccg acagccaaca agcggcactt gccgtaacga tcccaaaatg cagagctcgg
    15301 gccttcaagg gtcgaaccga tagcaggagc cagccgtaca agcaatgaag tccggcgatt
    15361 ccaggacacc ggcccacatt ctaggtgtat ctggagattt ttccagaatg ctgttcaatc
    15421 tcgctgggcc cctacaccga aagaaataac gaatccagat gatgggcggt gggccgtcgc
    15481 ggtttgaagg ccggaaagcc ggacacacag accgtcgggc aggatccgag aatgcgtgcg
    15541 accgcacccg acccgcagtc tgacaacagg tagcacaggc tgtgcccgat ctggaggaga
    15601 cctctcacgt tttcggctcg tcccagatgc cgagatcgtg agtacggcct ataactgaca
    15661 agcaacgaga gaattgggaa tgcgtgggag attctctacg ctttgttggt cagctttatt
    15721 ggtcacacta aggagttgga ggcatcgcca tggttacaca attgcctgac ggcaccgtcg
    15781 agtttcggtt ttatcgcccg ggagcccgac acgtaactct cgcaggtgac ttcaacggct
    15841 ggaatacaac cagcttagca atgcaaatgg acaaagacgg atggtggcga tacgtcatcc
    15901 atcttacccc cggctgttac cagttccgct atgtggccga cggcgagtgg cacaccgact
    15961 acgccgcgtt cggactcgaa catggaccgt tcggggtcaa ctcggtcgtc aaagttgaac
    16021 ctgctccggc ttcgagaacg gccacagcgc gacctccagt ccttcgcttc gtcaacagcc
    16081 gggaagccga cgagatcatg ctggcccccg gcgccggccg acaactcgac tggtcagtgg
    16141 acgagacaga agaggaactc gcagaggcgt ttgcctgatc gtcacgcgat tagagggatc
    16201 cgccccccgg gaaccacgct gtggctgcgc ggggggcttc ttgttggctt gacgctgccg
    16261 tgcagggccg gtaccctatc gagtgtgcgg atcgcgggcc cccaacgccc cgaatgccga
    16321 acaaaaacct agcccgggtg gcggaattgg cagacgcgca tggttgaggg ccatgtccca
    16381 gtaatggggt ggaggttcga gccctctccc gggcattccc ggtcggacca ctcctccgtc
    16441 ttacgcagat agacaattgg tgcaacggcc tgccgatcag cccgaaaacc ctgccgacgc
    16501 gccaatcaat ccctccccct cttcacccag cttggtgtca ccgggcaggt tgaatttcgg
    16561 ccacctgcgg gcggcttcgg ctgtactctt catttcccca aggcggcctt tggccgcaac
    16621 caaagcattt ctgagtggcc cgtatggggc cggggtcaac tcttgactct tgacatttcg
    16681 gggccgatgt ggtaggggtg tcgg
//
LOCUS       DAOTPW010000121        16624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3459_length_16624_cov_4.993603, whole genome shotgun sequence.
ACCESSION   DAOTPW010000121 DAOTPW010000000
VERSION     DAOTPW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16624
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3459_length_16624_cov_4.993603"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="P5316_12130"
     CDS             <1..1976
                     /locus_tag="P5316_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRR85746.1"
                     /translation="AIGLLVDDAIVVVENVERIMSEEGLSPREATKKSMGQITSALIG
                     IGLVLSAVFGPMAFFAGSTGVIYRQFSITIISSMMLSVVVALILTPVLCASLLKPVPA
                     GHEPAEAAIFFLRPFFRLFDRIFFRIRDIYVGIVGRSFGKKLRYGLVYVLIVSAVGFL
                     FRHLPTGYVPDEDQGMMLAQIILPAGSTLEQTQGVLEEVRTHFQENEKEAVESCMTIC
                     GVSFSGRSQANGMVFIKLKDWGLRDRPDLRVKAVAERATKTFSRMRHALVFAFPPPAV
                     VELGNARGVDFQLLDRGGLGHEKLMQARNQFLALAAQDPRLSVVRPNGLEDVPEYRLD
                     VDWERAGALGVPITSIHNTISAAFGGAYVNDFVQAGRVKRVYVQLDAPHRMLPKDLGK
                     TYVRNNVGKMVPLSAFASGRWAYGPARLERYNGFPSMNIWGEPAPGKSSGDAMRAMEE
                     IVEKLPQGVGFDWTGLSYQERMASKQGPILYAFSILVIFLCVAALYESWPIPLANMLM
                     LPLGAFGATAATWLRGLPNDVYFQIGILTTLGLTTKNAILIIQFTQERLARGEGLIQA
                     TLGAVRVRFRPVIMTSLAFFFGVLPLAIATGAGAGAMNAIGTAVTGGMLSATFIDLLF
                     IPLSFVLVSRLFKVEERARRAASQQATSELATG"
     gene            complement(2227..2763)
                     /locus_tag="P5316_12135"
     CDS             complement(2227..2763)
                     /locus_tag="P5316_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85747.1"
                     /translation="MGSGGRERRTVRRGVNLVELLLVVIIIGIIASIAIPRASRGSRI
                     TTEPELVNCLAILRMAIEEYAADHEGRFPGTNPDRTAGDPKLFADQLTKFTSVAGEVS
                     ETRTSIHRFGPYVHRRIPAVPVGTNKGSSQVAIDIDSLMPTVNLGNPAGWIYNPLTGD
                     IIANAEGADETGLLYCNY"
     gene            complement(3021..5723)
                     /locus_tag="P5316_12140"
     CDS             complement(3021..5723)
                     /locus_tag="P5316_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRR85748.1"
                     /translation="MSTSKRIGHYVASTHWDREWYEPFQDYRFRLVNLLDEVIDTMKT
                     DPDFRYFQMDGQVITIEDYLEIRPEREAEIRQLAQSGRLKLGPWYVLPDEFLVSGESI
                     IRNLQMGLRLAQRFGDPSRAGFLCDMFGHISQMPQILRGFGIDNAFLWRGVNEVTHGA
                     MFRWQSPDGSEVIAYRFSPRSGYCAYAFRVRHGHEPDVIFDIETATKDFREYLDFETA
                     RCPTSAFLVFDGGDHMEIEPQTTTLLAEANKRFADVEIISSTLDAFVEDIRKECGRIT
                     KVFSGELRDPAVLGDEQWLIPGVLSSRVHLKQANARCETELCLWAEPFSVFAARTGRP
                     YPEAFLNTAWRYLLQNHPHDSMCGCSIDQVHDDMLYRFNQSALIAAHVTRDALRHIAE
                     RVRLPEMGEKDIAVVVFNPCGEPIDGPVDLTLRFPECMDAVYQEWFGFEKKIGFRLYD
                     ADNRELPYQYVNQRQGRYGFRRPLRKFPVADVRHEVDVSVPLRVPAYGYTTIICRPVK
                     EPTRYPGTLAVDDHTIENEFLKIGVNSNGTLVLTDKRTGQVYDRLLTLEDCADIGDGW
                     YHGTAVNEQVFTSIGSSADIALTADGLCKATLTVRVTLNVPEQFQFGSKMARAGSLKP
                     LIVVHHVTLRKGGDAVEVRTEVDNTVRDHRLRVLFPTGCKATTYLADQAFDVVERPIA
                     LRADNAKLKEMEVETKPQQTWTAVYDERRGLAVITVGLRESAVRDQPERPIALTLLRS
                     FVRAVLTSGNEGGQIQGRHEFQYRIVPLSGRPDVARLCRMGQQLAAGVRAVQQEHRDI
                     VGDREGGPAGRTLPASQAFLTVAGRDAVVTALYRRHPGEDASVRVFNPTTRPMEVAIT
                     MPGTRGQAKLTDFAGRTIAAAPREGDNVCVSLRPKQIATIQWIG"
     gene            complement(5809..7125)
                     /locus_tag="P5316_12145"
     CDS             complement(5809..7125)
                     /locus_tag="P5316_12145"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRR85749.1"
                     /translation="MNEARTKSRPESLWGPVKTLIVLLSACVGCVSPNQRIAGIVPTD
                     WPADLPMPVEVPLPTMPVGVSPDGFGAKRLLVRVSIGHRPATLQPADLGRADPFASRG
                     EAVLRTYLKLKESDRGQPIEILMPDHRRAGAGVCCKARYEEPFLHVAAGPDKPILSYW
                     HGPANSKNAKSHPLNDFIHPVTGLDGEELTAASPGDHIHHRGIFWAWVRSERQGKWQN
                     EWWIPREFVAEPGRLEYDREGATSCRFTAEHFWTYRPADPARNERFVREMVDCRVYCG
                     SPSGRAVDIDLTLTALVDGIRIGGQTAADKGYGGMTCRFGSATEVRMENDRGPISGDS
                     LNHVVARWVDWSGRFIGPDGRRLPHRSGAALMVHPSHPTCPPEWIVRAYGPIGVAYPG
                     LEMIDLPENEPLHLRYRLWIHRGDAIAGKVPEAYQAYVADWQWKTG"
     gene            7500..9185
                     /gene="gspE"
                     /locus_tag="P5316_12150"
     CDS             7500..9185
                     /gene="gspE"
                     /locus_tag="P5316_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HRR85750.1"
                     /translation="MSVGEILLERGKIRPEHLQAAQAARKNPQERIDQILVRMGFVDE
                     RDVLEALGEQMSIAVVDLTQITIDKDLLKLMPTRLVHKRGLIPIEKTADTLRVATADP
                     FDFTALDELRMLTGLKVEAVLASASEIQRLIRQHFGVGGSTIDEMIEDQPEDEDVELL
                     SESVDENGDLVEMAQEATVVRLVNEILTEAIRDKASDIHIEPYEHTLKIRYRIDGVLQ
                     NTVLPPKIERFQAAIISRIKIMSNLNIAEKRLPQDGGFKARIHGREIDFRVSIIPTGY
                     GEAVVMRILDRQSINLSLQQLGMGPDVLSQFQRLINMPHGIILVTGPTGSGKTTTLYA
                     ALHTIVSDEIKILTIEDPIEYYLDGVNQVQTSEKIGLNFARALRSFLRHDPDVILVGE
                     IRDRETAEVAINASLTGHLVFSTLHTNDSAGATTRLLDMGVEPFLVSSSLEGILAQRL
                     VRTICPHCKEAYKPDRDALPQDFVIEKGQVLYRGVGCRECRQIGYRGRIGIYELLVMN
                     DELRELVVQRASAGRIRQVAMQNGLRLLRQDGWDKVLAGHTTCEEVLRVTNVG"
     gene            9585..11582
                     /locus_tag="P5316_12155"
     CDS             9585..11582
                     /locus_tag="P5316_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRR85751.1"
                     /translation="MTTITRIAGGLAMGLMVLWSSGCGRNVVPDGGNDGGPFDHYVPD
                     PNVYAGAVDPAMIIEQTTGTGATIRFAVNQLVLLMNEGTTRSTVEALAHRLGGTIVGQ
                     VPDIDFYQIELSTTTRAQLDAAVAQAEEDPNVTAAGYNVASRLNQSCPAANDNAEIFN
                     IDKCAFTETEYYNALTMFDFFRPHLALNPVTVAVIDSGLDPTTGEFDNTDVFYLNNLR
                     GPPVDSDPDRHGTMVCGMIAADDDMQGINGIASRFLLDNLSLAVAGWEGIQLGTSATG
                     SCVAHEMLYTFVAAAAGAQVINLSIGYEPSDPHFRVIRETWKRRIARLPGVVFVAAAG
                     NETTELDGNNSAPGGIGLPNLITVAGTARCEPDRPASFTNYGPVVDVAAPAEDVPVVF
                     DAVYVTGSGTSFATPMVASLAAILKSIQPTLTPAQVKHYIVDCAHPLYGNPFGRLMFT
                     NSICQLLIDMGVGDPIRSWIDPMGLGSFGASGLVLSRICPAGIVYTVDGYGTYQTYYE
                     TESDHRPAIAGSFGGTSFTCGGGDGDASIGFTSLSLTQFVLGTYIITTPEEAGPDTAA
                     ASFTDFTTGESGMGMAGSITFDACQIDQRSPFDGINPWIVRLSGAFAGVLQMAHNDRA
                     PTFHEFEGSFSVPLLVLAGAEDSFIDYLENTCEGGIPRPHE"
     gene            11761..11919
                     /locus_tag="P5316_12160"
     CDS             11761..11919
                     /locus_tag="P5316_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85752.1"
                     /translation="MGKETEPAVHLAHPGRRTRFCCKPGKNRFEANPAKYLAKLGRQL
                     STVTKPTS"
     gene            complement(11945..12787)
                     /locus_tag="P5316_12165"
     CDS             complement(11945..12787)
                     /locus_tag="P5316_12165"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85753.1"
                     /translation="MTPFKTAPLSDDRRWIVEGCLTTGLKLAAAAWLGLWLVPSANAQ
                     DCNFNDVPEAQDIAVGTSQECNGNGLPDECEMYEEAKLTASDTESQDDFGYFLSLDGD
                     LLVVGAYGDNDRGTGSGSIYLFRHIRAGWIAWAKPTAADGTAGDSFGAVDSRGQTLVV
                     GGPGWLQNVGSAYAYRLQDLDWDRTPDTCDLCPATIGGVLVDINGCPPFVPGDFDRDG
                     DVDMNDLAVFEACATAPAISQPSPDCVWACFDTDDDIDQADFAVFQRCLSGEKPAADP
                     NCAS"
     gene            complement(13051..14931)
                     /locus_tag="P5316_12170"
     CDS             complement(13051..14931)
                     /locus_tag="P5316_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR85754.1"
                     /translation="MSRNMGRFALLLAVTVSASLFAGTAWAAVSITGHGRYEKIKGKP
                     GMGYVELYEANLFLSPPSSSFVGPSSRLGGSAYYTGTACVSGTTHDGVYCFENVPAGQ
                     YSILVNQPLFYIAPKVVSNISITSGQTVTVNPELPIDYSTYFKNAWTGPANRWYQTFV
                     ATGISIRGIMFAYAGDDPDNVEVAVLKDNGNANVVNWQFIGARIEPQVGDVTDNWVRW
                     RSGEIPTVPGARYAVRLTANGGSGNLQPYKRDKDANSYANGRAYDAQGNPQNFDLNIT
                     VFSDNDGTIVTMNRRVTGLGYLCDGGFFGPRWGQTFVAKGKGLAGADVWAAGAERKWN
                     LEFLWKVRQGGPTGPQIGPDKITLAAYQAFGVGLHGVSYNPGEVPLTRGQTYFIEFNI
                     HNPPPESPGFNPYLMKPASGASCGGGDDHDSYADGIAYRDNVPRPDDDLAMTIIEYAI
                     QGPEITLNKDTLAHSVFLGDSQGNDTFTIVNGGDGTLNYAIEEIPPVSWLDVTPISGT
                     ATTETDTITVSYTTASLPVGHYTTVIRVSGNAANSPQDIAVTIDVHSVKADYDGDGDV
                     DQDDFGRLQKCYGGAGVIITDPDCADVSLDSDEDVDGDDFLVFENCISGSDVKADPNC
                     AG"
     gene            15414..16379
                     /locus_tag="P5316_12175"
     CDS             15414..16379
                     /locus_tag="P5316_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRR85755.1"
                     /translation="MAAPVFIEPENGYTPDPMRKQYNILDRRLSEVQEEHAHVTIFSN
                     PDENEKRYLIDELKVDEHTFNSALDPDELSRLEFEPEHVAVIFKRPKNYSGGEQFLFK
                     VGSVGAFLFKDRVKIVVSEDVPLFDGVPLTRVHSPADVILKLIARSVIHFREHLKGIS
                     MISDELQKEINTSMENKHLINMFTLEKSLVYYVNAINSNGAVLERLKNNAARLGFSTE
                     EVEFLDDLIIENNQCYRQAEMYSNILASLMDARASIVSNNINVLMKTLNIITIAIMVP
                     TFVVSAFSMNVAIPMQHLPYAFEIIMGLALLSVLLFMLCWRRYRP"
BASE COUNT         3238 a         5251 c         4867 g         3268 t
ORIGIN      
        1 tggccatcgg gctgctggtg gacgacgcca tcgtcgtggt ggaaaacgtg gagcggatca
       61 tgagcgagga gggactttcg cccagggagg ccaccaagaa atcgatggga cagatcacca
      121 gcgctctgat cggtatcggc ctggttctct cggcggtttt cggccctatg gccttcttcg
      181 cgggttcaac cggtgtgatc tatcgacagt tctccattac cattatctcc tccatgatgc
      241 tgtcggtggt tgtggcactg attttgactc ccgttttgtg tgcttccctg ctcaagccgg
      301 taccggctgg gcatgagccg gccgaggccg cgatcttctt cctccgtcca ttcttcaggc
      361 tgttcgatcg catcttcttc aggatccggg acatctatgt cgggatcgtc gggcggtctt
      421 tcgggaagaa actgcgttac gggttggtct acgtcctgat cgtctcagcc gtcgggtttc
      481 tcttccgtca tctgcccacc ggctacgtgc ccgacgaaga tcaggggatg atgctcgctc
      541 aaatcattct gcccgccggg tccacgctag agcagactca aggagtcctg gaggaggttc
      601 gcacccactt ccaggaaaat gagaaggaag cggttgaatc ctgcatgacc atttgcgggg
      661 tcagcttctc gggaaggtcc caagccaacg gcatggtctt catcaagctc aaggactggg
      721 gtctccgcga ccggccggac ttgagggtaa aagccgttgc cgaacgggct accaagacct
      781 tctcccggat gcgccatgcc ttggtgttcg cctttccgcc gcctgccgtg gtcgaactcg
      841 gtaacgccag gggcgttgac tttcagttgc tcgaccgggg cggcctgggc cacgaaaagc
      901 tgatgcaggc ccgcaaccag tttctggcgt tggccgccca ggacccaagg ctgtccgtcg
      961 ttcgccccaa cggcctggag gatgtgcccg aataccggct cgacgtggat tgggagcggg
     1021 ccggagccct tggcgtcccg atcacctcca ttcataacac catctcggca gctttcggcg
     1081 gggcttacgt gaacgacttc gttcaggctg gtcgagtcaa gcgggtctat gttcaactcg
     1141 atgctcctca ccgcatgttg cccaaggatc tgggcaagac ctacgtccgc aacaacgtgg
     1201 gtaagatggt acccttgtcc gctttcgcgt ccggccgctg ggcgtacggc cctgcccgac
     1261 tggagcgcta caacgggttt ccctccatga acatctgggg cgagccggct cccggtaaga
     1321 gctccggcga cgcgatgcgg gccatggaag agatcgttga gaagctgcct cagggggtgg
     1381 gctttgactg gaccggcctg tcctatcagg aacgaatggc cagtaaacag ggacccatcc
     1441 tgtacgcctt ttccatcctg gtcatcttcc tgtgcgtggc cgccctgtat gaaagctggc
     1501 ccatcccgct ggcaaacatg ctgatgctgc cgctgggggc gttcggcgcg actgcggcca
     1561 catggctgcg ggggctgccc aacgacgttt attttcagat cgggattctc accaccctcg
     1621 ggttgaccac caagaacgcc atcctgatca ttcagttcac gcaggagcgc ttggctcgcg
     1681 gagaaggatt gatccaggcg accctcgggg ccgtgagagt ccggttccgg ccggtcatca
     1741 tgacttcgct ggctttcttt ttcggcgtct tgccgttggc tattgccacc ggtgcgggcg
     1801 ccggcgcgat gaatgccatc ggcaccgcgg tgaccggcgg aatgctctcc gcgacgttca
     1861 tcgatcttct gttcattccg ctgtcctttg ttctcgtgtc gcgattgttc aaagtggaag
     1921 aacgcgctcg aagggcggct tcgcagcaag cgacgagcga actcgcaacc ggatgacggc
     1981 agcacgggcg caggctgcac acacgttgtc gtcctgggtc gaaaggggga tggcttcttc
     2041 gccggcgtgt ttttgcttgc gggtgcttgg ccgacggacg tccagaaaca agcggccgtg
     2101 agttccgtcg tgaatccggc tcacggccgc ctgcgctgtc ggtccccaga taccaggaga
     2161 tattaggggg cccgattcgg gtctcgtcga actcgccgac cctcgggtcg aacgaggatt
     2221 ctgtaatcag tagttgcagt acagcaatcc tgtctcgtca gccccctcag cattggcgat
     2281 gatgtcgccg gtcagggggt tgtagatcca tccggccggg ttgccaagat tcaccgtggg
     2341 catcaagctg tcgatatcga tggccacctg cgatgatccc ttgttggtcc ccaccggaac
     2401 ggccggaatc cggcggtgga cgtacgggcc gaatcgatgg atcgaagtcc gcgtctccga
     2461 tacttcgccg gcgaccgacg tgaacttcgt caactggtcg gcaaagagct tcggatcgcc
     2521 ggccgtccga tccggattcg ttcctggaaa tcgcccttca tgatccgcgg catactcctc
     2581 aatcgccatc cgcagaattg ccaggcagtt gaccaactcg ggttcagtcg tgatgcgtga
     2641 cccgcgactg gcccgcggga tggctatcga ggcgatgatg ccgataatga tgacaacgag
     2701 gagcaactcg accagattca ccccccggcg caccgtccgc ctttcccttc cgcccgaacc
     2761 catgtttacc ggctcctccg caacgatcaa ccggcgcaga accatcgcag agtcatctca
     2821 agtcgagcgc gcctgctcgt gcggttcgtc accaggttct tcgactaaca cgccggtttg
     2881 ctttacttcc cactgctcgc ccggccctcg ggtgtggccc agggtggcga ggatgcccca
     2941 taaacacggg aaaggaacac ggaccaccac aacctccaat tacgccgcgc atgtgtccga
     3001 tctcgccggc ccggaggtta tcagccgatc cactgaatcg tggcgatctg cttcggcctg
     3061 agactgacgc aaacgttgtc tccctcacga ggtgctgcgg cgatcgtcct ccccgcaaaa
     3121 tcggtgagct tcgcctgtcc ccgggtgccg ggcatggtga tggcaacctc catcgggcgg
     3181 gtggtcggat tgaagacccg aacggatgca tcctcgcccg gatgccggcg gtaaagagcg
     3241 gtcaccaccg cgtcccgtcc cgccacggtc aagaaggctt gcgaggcggg cagcgttcgc
     3301 cccgccggcc cgccctcccg atcaccaaca atgtcgcggt gctcctgttg caccgcccga
     3361 acgccggcgg caagttgctg ccccatgcgg cacaggcggg cgacgtcagg tctgcctgac
     3421 aacggtacga ttcggtactg gaactcatgc cgcccttgga tctgcccgcc ttcgttgccg
     3481 ctggtcagga ccgcccggac aaatgaacgc aacaaggtca aagcgatcgg ccgctcaggc
     3541 tggtcgcgca ccgcggattc gcgcaagccc acggtgatca ccgcaaggcc ccgacgctcg
     3601 tcatagacgg ccgtccaggt ctgttgcggc ttggtctcga cctccatctc cttcagcttc
     3661 gcgttgtcgg cccgcagagc aatggggcgc tccacgacgt caaacgcctg gtcggccagg
     3721 tacgtcgtcg ccttgcagcc cgtcgggaac agtacccgta accggtgatc gcgcacggtg
     3781 ttatccacct cggtgcggac ctcgaccgcg tcgccgcctt tgcgcagcgt cacgtggtga
     3841 acgacgatca acggcttcaa cgacccggcg cgggccatct ttgagccaaa ctggaactgc
     3901 tccggaacgt tgagggtgac ccggacggtc agtgtcgcct tgcagagtcc gtcggcggta
     3961 agggcgatgt cggccgacga accgatcgag gtgaaaacct gttcgttcac tgccgtgccg
     4021 tgataccagc cgtctccgat gtcggcacag tcttcgagcg tcaacaggcg gtcatagacc
     4081 tgtcccgttc tcttgtcggt cagaaccagc gtgccattgg agttcacgcc gatcttgagg
     4141 aactcgttct cgatcgtatg gtcgtcgacc gccagtgtgc ccggatagcg cgtgggctcc
     4201 ttgaccggcc gacagatgat ggtggtgtag ccgtatgcgg gcactcgcaa gggaacgctc
     4261 acgtccactt cgtgccggac gtccgctacc gggaacttgc gcagcggacg tcggaaaccg
     4321 tatcgccctt ggcgctgatt gacgtattga tacggcagtt cgcggttgtc ggcatcgtaa
     4381 agcctgaaac cgatcttctt ctcgaacccg aaccactcct gatagacggc gtccatgcac
     4441 tcgggaaacc gcagcgtcag atcgaccggt ccgtcgatcg gctctccgca aggattgaag
     4501 accaccacgg cgatgtcctt ctcccccatc tccgggagcc ggacccgctc ggcaatgtga
     4561 cgcagcgcat cccgtgtaac gtgggcggcg atcaatgccg attgattgaa gcggtaaagc
     4621 atgtcgtcgt ggacctgatc aatgctgcag ccgcacatcg aatcgtgcgg atggttctgg
     4681 agcaggtatc gccaggcggt gttgaggaac gcctccggat acgggcggcc cgtgcgcgcg
     4741 gcgaaaacgc tgaacggctc cgcccacagg cataactcgg tttcgcaccg ggcattggcc
     4801 tgcttgagat gaacgcggct gctgagcacc cccgggatca gccattgttc gtcgccgagc
     4861 accgccggat cgcgcagctc ccctgaaaac accttcgtga tcctgccgca ctccttgcga
     4921 atgtcctcca cgaacgcgtc aagagtcgaa ctgatgatct ccacatcggc aaaccgcttg
     4981 ttggcttcgg ccagcagcgt ggtcgtttgc ggctcgatct ccatgtgatc gccgccgtca
     5041 aagaccagaa aggcgctcgt cgggcaccgg gcggtctcaa agtccagata ctcgcggaaa
     5101 tcctttgtcg ccgtctcgat gtcgaagatc acgtcgggct cgtgaccatg tcgtaccctg
     5161 aaggcgtagg cgcagtaacc gctgcgcggg ctgaaccggt aggcgatcac ttcgctcccg
     5221 tccggcgact gccagcgaaa catcgccccg tgggtcactt cgttaacccc tcgccagaga
     5281 aaggcgttat cgatcccgaa gccgcgcagg atctgaggca tctgcgaaat gtggccgaac
     5341 atgtcgcaca aaaaccccgc tctcgatggg tcgccgaacc gctgcgccaa cctcaaaccc
     5401 atctgcagat tgcggatgat cgattcgccg ctcaccagga actcatccgg cagaacgtac
     5461 cagggtccca gcttgagacg gccggattgc gccagttgcc gaatctcggc ctcccgctca
     5521 ggccgaatct ccaggtaatc ctcgatcgtg atgacctgcc cgtccatctg gaaatacctg
     5581 aaatcgggat cggtcttcat cgtgtcgatg acttcgtcca gcaggttgac caggcgaaaa
     5641 cgatagtcct gaaagggttc gtaccattcg cggtcccagt gcgtgctggc cacgtaatgt
     5701 ccgatgcgtt tcgatgtaga caagctgagg cctcctgttg ctgattcttg tctcttgggg
     5761 ctgaagatca taagccgaca gggcggaagg atgccaaccg accggtatct accccgtctt
     5821 ccattgccag tcggcgacgt aggcctgata tgcctccgga accttcccag cgatcgcgtc
     5881 gccccggtgg atccagagcc gatatcgcag atgaagcggt tcgttctcag gcagatcgat
     5941 catttcgaga cccgggtacg ccacgccgat cggaccgtag gcgcgcacga tccattccgg
     6001 aggacacgtc gggtgcgagg ggtgaaccat cagggcagcg ccgctccgat gcgggagacg
     6061 ccgcccgtct ggtccgatga acctgccgct ccaatcgacc cacctcgcga ccacgtggtt
     6121 caggctgtcg ccactgatcg ggccccggtc gttttccatc cggacctccg tcgctgaccc
     6181 gaaacggcac gtcattccgc cgtatccctt gtccgcggct gtctgtcccc caatccgaat
     6241 gccatcaaca agcgcggtca gcgtcagatc gatatccact gcccgcccgc tcggcgagcc
     6301 gcaataaacc cggcaatcca ccatttctcg aacaaatctc tcgtttcggg ccggatcggc
     6361 gggccggtac gtccagaagt gctccgccgt gaaacggcac gatgtcgctc cctctcggtc
     6421 atattcgagg cgcccgggtt cggcgacgaa ctccctcggt atccaccact cattctgcca
     6481 tttgccttgt cgttccgagc gaacccaggc ccagaagatc ccgcgatgat gtatgtggtc
     6541 ccccggcgaa gccgccgtca gttcctcgcc atcaagcccg gtcaccggat gaatgaagtc
     6601 gttcagagga tggctcttgg cgttctttga gtttgcagga ccgtgccagt aggacaatat
     6661 cggtttgtcc ggccctgcgg caacgtggag aaaaggctcc tcgtaccgtg ccttgcagca
     6721 cacgccggcc ccggccctcc gatgatccgg catgaggatc tcaatcggtt gtccccggtc
     6781 cgactccttg agcttgagat atgttctcaa tactgcttcg ccgcgggagg caaagggatc
     6841 ggcccggcca aggtcggcgg gttggagtgt ggccgggcgg tgcccgatgg aaacccgcac
     6901 cagcagccgc ttggccccga acccatcagg tgataccccg acgggcattg tggggagagg
     6961 cacttcgacg ggcattggca ggtccgccgg ccaatccgtt ggcacgatac ccgcaatcct
     7021 ctggtttgga ctcacacagc caacgcaggc actcagcagt acgatgagcg ttttgactgg
     7081 tccccaaaga ctctcgggcc gactcttggt gcgagcttcg ttcataattc tgtgtttccc
     7141 gcgctatgag acctcggcat cgcatgatac cgggcgtggg tattggcgca caggtgctcg
     7201 tgaggcgtcg tcggccgata accaagcggg cgagtcgcga tggtcaatcg gagcataaat
     7261 gtttgcaggt aaatagatta tcaacatcaa ccgatataaa gaaggaccgg cggcgaaatg
     7321 tcgtctcgcg gtctgggacg gtttttggga tgggccctgc aacttttccc gacggccgct
     7381 ttataagcaa tctggagacc gggggtgagg tgcgcccacg ccgacgatca gcggcggggc
     7441 gccgaaccag aaccggttgt tgggacacgg gtaattagcg ggcttgtggg gattgaccag
     7501 tgagtgtcgg cgaaatcctg cttgagcggg gcaagatccg gccggagcat ctccaggcag
     7561 cccaagcggc gcgcaagaac ccacaggaac gaatcgatca gatcctcgtt cgcatgggtt
     7621 tcgtggacga gcgagacgtg ctcgaggcgc tcggcgagca gatgagcatc gcggtggtgg
     7681 acctcacgca gatcaccatc gacaaagatc tgctcaagct gatgccaaca cgcctggttc
     7741 acaagcgagg cctgattccc atcgaaaaga ccgcggacac ccttcgggta gccaccgcgg
     7801 atccttttga cttcacggcc ctggatgaat tgcgcatgct taccggcctg aaggtcgagg
     7861 ccgtgctggc cagcgcaagc gagatccagc ggctgatccg ccagcacttc ggcgtcggcg
     7921 gttccaccat cgacgagatg atcgaggacc agccggaaga cgaagacgtc gagctgctga
     7981 gcgaatcagt tgacgaaaac ggcgacctgg tcgagatggc ccaggaagcc acggtcgtcc
     8041 gcctggtcaa cgaaatcctg accgaagcca ttcgcgacaa ggccagcgat attcacatcg
     8101 agccgtacga gcataccctc aagatccggt atcgaatcga cggggttctg cagaacaccg
     8161 tgttgccgcc caagatcgag cgatttcagg cggccatcat cagccgcatt aagatcatga
     8221 gcaacctgaa tatcgccgaa aagcgtcttc cgcaggacgg cggtttcaag gctcggattc
     8281 acggccgcga aatcgacttt cgcgtctcga tcatcccaac aggttacggc gaggcggtgg
     8341 tcatgcgtat cctcgaccgg caatccatca acctgtcgct ccagcagttg ggcatggggc
     8401 cggatgtgct cagccagttc cagcggctga tcaacatgcc ccacggcatc atcctggtca
     8461 ccgggccgac cggcagcgga aagacgacca cgctgtacgc cgcattgcat accatcgttt
     8521 ccgatgagat caagatcctc acgatcgaag atcccatcga gtattacctc gacggcgtca
     8581 accaggtgca gacgagcgag aagatcggcc tcaacttcgc ccgggcgctt cgttcgttcc
     8641 tgcgtcacga ccctgacgtg atcctggtcg gcgaaatccg cgaccgcgag accgccgagg
     8701 tggcgatcaa cgcctcactg accggtcacc ttgtgttcag caccctccac accaacgact
     8761 cggccggcgc aaccacgcgt ctcctggaca tgggcgtcga gcccttcttg gtctccagct
     8821 ccctcgaggg catcctggct cagcgactcg tgcggaccat ctgccctcac tgcaaggagg
     8881 cctataagcc cgaccgcgac gccctgccgc aggatttcgt catcgagaag gggcaggtcc
     8941 tgtaccgcgg cgtcggctgc cgcgaatgcc ggcagatcgg ctatcgtggg cgtatcggaa
     9001 tctacgaact gctggtgatg aacgacgaac tgcgcgaact ggtggtccaa cgggcttccg
     9061 ccgggcgaat ccgacaggtc gcgatgcaaa acggcctgcg gctgctccgc caggacggct
     9121 gggacaaggt cctcgccggc cacaccacct gcgaagaggt cctccgcgtc accaatgtcg
     9181 ggtgatgttc gatcataccg ctgaaactgg ctgcgcacac cgatcatgaa gagggaaaac
     9241 acacaagact tcgaacgtgg acttatggcc caagaccgcg gtatgtcttc cttgagaagc
     9301 agcacttgag gcaaatacgg tattccgaca taaggtccgt gtgccttcag gaccgacgag
     9361 cgacttcgaa ccgcttcaga aaacgaaacg agactcttga tccgcgccgg ctctccaagt
     9421 acacttaata tcggtcactg ctggcaatgg cgggccgcat ctgacgagtt cacttcttgc
     9481 ttcgcggcgg tccaggccgg gaacggttga gccggctgtc gccaatgggg ccggtgcaaa
     9541 tgaagcattc ttcttcatga tccgttgatc gaccggaggc tcccatgacc acgatcaccc
     9601 gcattgccgg tgggcttgcg atgggactga tggtgctctg gtcgtcgggt tgcggcagga
     9661 atgtcgtgcc cgatggcggc aacgacggtg gaccgtttga ccactatgtg ccggatccga
     9721 atgtttacgc cggcgctgtc gacccggcca tgatcatcga gcaaaccacc ggcaccggtg
     9781 caactatccg tttcgcggtc aaccagctcg tgctcctgat gaacgagggc actacccgtt
     9841 cgacggtcga ggcgttggcc caccggcttg gcggcaccat cgtgggccag gtgccggaca
     9901 ttgatttcta tcagatcgag ctgtccacga cgacacgggc gcaactcgat gcggccgttg
     9961 ctcaggccga ggaggacccg aacgtcaccg ccgcgggcta caacgtggcc tcacgcttga
    10021 accagagctg tccggcggcg aacgacaatg ctgaaatctt caacatcgac aagtgtgcgt
    10081 tcaccgaaac ggaatactac aacgccctga ccatgtttga tttcttccgc ccgcacctcg
    10141 cgctgaatcc ggtcactgtg gccgtgatag attccgggct ggacccgacg accggagagt
    10201 tcgacaatac ggatgtcttt tatctcaaca acctgcgcgg acctcccgtt gacagcgatc
    10261 cagacaggca cggcacgatg gtatgcggca tgatcgctgc cgacgacgac atgcaaggaa
    10321 tcaacggcat cgcgtcgcgg ttcttgctgg acaacctgag tctggcggtg gccggttggg
    10381 agggtatcca gcttggcacc tcggccacag ggagctgcgt cgcccacgag atgctttaca
    10441 cgttcgtggc cgcagcagcg ggggcacagg tcatcaacct gagcatcggc tacgaaccgt
    10501 ccgacccgca tttcagagtg atccgggaaa cctggaagag aaggatcgcc cgcctgcctg
    10561 gtgtcgtgtt tgtggctgcg gcgggaaacg agaccaccga gttggacgga aacaatagcg
    10621 cccccggcgg catcggcctg cctaatctga tcaccgtggc gggcacggcc cgctgtgagc
    10681 ctgacaggcc tgccagtttc accaactacg gcccggtcgt cgatgtcgcc gcacccgccg
    10741 aggatgttcc cgtggtcttc gacgctgtgt acgtgaccgg ttcaggcacg tcctttgcaa
    10801 cgcccatggt tgcatcgctg gcggcgattc tcaagtcaat ccagcccaca cttacacccg
    10861 ctcaggtcaa gcactacatt gtcgattgcg cccaccccct ctatggcaat cccttcggcc
    10921 gcctgatgtt cacgaactca atctgccaat tgctgatcga tatgggtgta ggcgatccca
    10981 ttcgctcctg gatcgatccc atgggtctgg gcagttttgg cgcctccggc ctggtgctga
    11041 gcaggatatg tccggcgggg atcgtctaca cggttgatgg ctacggcacc taccagacct
    11101 actacgagac ggaatcagac caccgccccg ccatcgcggg gtccttcggc ggcacatcgt
    11161 tcacgtgcgg cggcggagac ggcgacgcct ccattggttt tacgagtctg agcctgactc
    11221 aattcgtcct cggcacctac atcattacga cccccgagga ggccggtccg gacacggccg
    11281 ccgcatcgtt caccgacttt actaccggcg agagcggcat gggtatggcc gggtcaatca
    11341 ccttcgacgc ctgccaaatc gaccagcgca gtccgttcga cggcatcaac ccatggatcg
    11401 tgcggctgag cggggcgttc gcaggcgtac tccagatggc ccacaacgac cgcgccccca
    11461 cgtttcatga gttcgaaggg tcctttagtg tgcctctcct ggtgctggcc ggagcggaag
    11521 actccttcat tgactacctc gagaacacgt gcgaaggagg cattccccgc ccgcacgaat
    11581 agaccgcatc cccgccccac aagagatgaa atgacgaccg ggcacatatg ctccatgtca
    11641 gcgatggtgc gttcgtcaac gcaacgggta aacgcgtgtc aaccacaagg caggtaacgg
    11701 ctctgctgta tcgcccactc atccccttgg ccggctgtcg ccccgaagac ctccccggca
    11761 atgggcaaag aaaccgagcc ggccgtccat ctcgctcatc ccggccggcg gacgcgcttc
    11821 tgctgcaagc ccgggaagaa caggttcgag gccaaccccg ccaagtacct ggccaagctg
    11881 ggccggcagt tgtcgacagt caccaagccg acaagttgag tggtctgcca tcgccgcgtc
    11941 cgaatcaact cgcgcagttc ggatcggccg ccggcttctc cccgctgaga caccgttgaa
    12001 agacggcgaa gtccgcctgg tcgatgtcat cgtcggtgtc gaagcaggcc cacacgcaat
    12061 ccgggcttgg ctgcgaaatg gccggggccg tcgcgcaggc ctcgaaaacc gctaagtcat
    12121 tcatgtccac gtcgccgtcc cggtcgaagt cgcccggcac gaacggcggg caaccattga
    12181 tgtcgacgag caccccaccg atcgtggcgg ggcaaaggtc gcaggtatcc ggcgtgcggt
    12241 cccagtcgag atcctgaaga cgatacgcgt aggccgaacc gacgttctgc aaccaacccg
    12301 gcccgccaac gaccagggtc tgtccgcgcg aatctacggc accgaaggag tcacctgcgg
    12361 tgccatcggc ggccgtcggt ttcgcccatg caatccagcc ggccctgatg tgccggaaga
    12421 gatagatcga tccggaaccg gttccacggt cattgtcgcc gtaggcaccg acaaccaaga
    12481 gatcaccatc gagcgaaagg aagtagccaa aatcgtcctg cgactcggtg tcggatgccg
    12541 tcagcttggc ctcttcgtac atctcgcact cgtcgggaag gccgttgccg ttgcactcct
    12601 ggcttgtgcc gaccgcaatg tcctgagcct cgggaacatc gttgaaattg cagtcttgcg
    12661 catttgcaga cggcaccagc cacagcccaa gccacgccgc ggctgcaagc ttcaagccgg
    12721 tcgtgaggca cccttccacg atccacctcc gatcatcgct caacggcgcc gtcttgaacg
    12781 gagtcataca gaaacatgat ccttccggcc cagaatctga tcagcccacc gaatgctgct
    12841 gccattgtac agcgctatgc gcccttgtac aaccctatgt agcgttagat cttctcattc
    12901 cctccgcttg aaataagcgc ataagacctt ccaaaagacg attctcaagc gtttcaagag
    12961 gtgcttttgc gccgctatga cactcgggtc acgatcatac catcgcaacc cgaagatgag
    13021 gcgctctgaa agcggcaaga ggggtcggtc tcacccggcg cagttggggt ctgccttcac
    13081 atcgctgccg ctgatgcaat tctcgaagac gagaaaatca tcgccgtcca cgtcctcatc
    13141 gctgtccagc gaaacatccg cacagtcggg atcggtgatg atcacgcctg caccaccgta
    13201 gcatttctgc aggcggccga agtcgtcctg gtcaacgtca ccgtcgccgt cataatcggc
    13261 cttgacactg tggacgtcga tcgtcacggc aatatcctgc gggctgttgg cggcattgcc
    13321 gctgacgcgg atgacggtgg tgtagtgccc gacgggcaag gaggccgtcg tataactgac
    13381 tgtgatggtg tcggtttcgg tcgtggcggt tccgctgatc ggggtgacat cgagccagct
    13441 caccggagga atctcctcga tcgcgtagtt gagcgttccg tccccgccgt tgacgatcgt
    13501 gaaggtgtcg ttgccttggg aatcgccaag gaaaacgctg tgtgccagcg tgtccttgtt
    13561 cagcgtgatc tccggaccct gaatggcgta ctcgatgatg gtcatggcca gatcatcatc
    13621 agggcgaggc acgttgtccc gataggcgat gccgtccgcg tagctgtcgt gatcatcgcc
    13681 accgccgcag gaagccccgg acgccggctt catcaggtaa ggattgaatc ccggggactc
    13741 gggcggcgga ttgtgaatgt tgaactcaat gaaataggtc tgccccctgg taagaggaac
    13801 ctcgccgggg ttgtagctga cgccgtgaag ccccacgccg aaagcctggt acgccgcgag
    13861 ggtgatcttg tccggtccta tctgcggccc cgtcgggcct ccctgccgga ccttccatag
    13921 gaactccaag ttccacttcc tttcggcccc cgccgcccaa acgtcggccc cggccaggcc
    13981 cttccccttg gcaacgaacg tttgtcccca gcggggcccg aaaaagcccc catcgcacag
    14041 gtagcccagt ccggtgaccc gcctgttcat cgtcacaatc gtgccgtcgt tgtcggagaa
    14101 cactgtgatg ttgaggtcaa agttctgcgg attgccctga gcatcatacg cccgcccgtt
    14161 ggcgtagctg ttggcatcct tatcgcgctt gtagggttgc agattgccgc tgccgccgtt
    14221 ggccgtcaga cgcaccgcat atcgcgcgcc cggcaccgtc gggatttctc ccgatcgcca
    14281 ccggacccag ttgtcagtca catcaccgac ctgcggctcg atgcgcgccc caatgaactg
    14341 ccagttgacc acgttggcgt ttccgttgtc cttgagcacc gccacctcga cattgtccgg
    14401 atcgtcgccg gcatacgcaa acatgatgcc gcggatcgaa atgcccgtgg ccacgaaggt
    14461 ctgataccag cggttcgccg gaccggtcca ggcattcttg aagtaagtcg aatagtcgat
    14521 gggcagctcc ggattgacgg tgaccgtttg accactggtg atgctgatgt tgctcaccac
    14581 cttgggagcg atgtaaaaca gaggctgatt gaccaagatc gagtactggc cggccggaac
    14641 gttttcaaag caatacacgc cgtcatgcgt cgtgccgctg acgcacgccg ttccggtgta
    14701 ataagcggat ccgcccagac ggctggaggg ccccacgaag gaactgctcg gcggcgagag
    14761 gaacagattc gcctcgtaga gctcgacgta ccccatgccg ggcttgcctt tgatcttctc
    14821 gtatcggccg tggccggtga ttgaaacggc ggcccaggcc gtcccggcaa acaagcttgc
    14881 cgaaactgtc accgccagca gcagggcaaa ccggcccatg tttcggctca tcaaagcacc
    14941 tccctcgaat gacctcaatt ccgcatgccg tcacaaaacg gtcgactcag atggatcggc
    15001 gatcctgttc atcctcgttg aatcaactcc gcgacatgaa acaagaatat cacaaggtcg
    15061 tcggacgggc aaatagcttc agtattactt tgatgatttc ttttgcggac gctgcatcca
    15121 ggcccgcgtc tcataccccc gcagccgacg acgaacaacc ggaccacgct tgccgcaaag
    15181 cggattacgc ccgcgtcggc agcggcgact ccgacagaaa gcgaggcttt ccagtccgtc
    15241 gctggttcat cgtggcacag gatcgcgccg taaccaagcc caacggtcac ggcccggccc
    15301 gtcaggttca ccgacaccgc tgctgaacgc ctccggggga cacggcggca atgggcgggc
    15361 gggaccgtat cgtcgcacca cggcagacgc ccgccccttc caggattccc ggcatggccg
    15421 cgcctgtttt cattgaaccg gaaaacggct acactccgga ccccatgagg aagcagtaca
    15481 acattctcga ccgccgattg tccgaggtgc aggaggagca tgcgcacgtc accattttta
    15541 gcaacccgga cgagaacgag aagcggtacc tcattgacga gctgaaggtc gacgagcaca
    15601 ccttcaatag cgctctcgac cccgacgaac tctcgcgatt ggagttcgag cccgaacacg
    15661 tggcagtgat tttcaagcgc ccgaaaaact actccggcgg agagcagttc ttgttcaagg
    15721 tcgggtcagt aggcgctttc ctgttcaagg atcgcgtcaa gatcgtcgta tcagaagacg
    15781 tgccgttgtt cgacggcgtg cccctgaccc gcgttcattc cccggcagat gtcattctca
    15841 agctgatcgc ccggtcggtc attcacttcc gcgagcatct caagggcatc agcatgatct
    15901 ccgacgagct gcagaaagag atcaacacct cgatggagaa caagcacctc atcaacatgt
    15961 tcaccctcga gaagagcctc gtctactacg tcaatgccat caattccaac ggtgccgtgc
    16021 tggagcggct gaagaacaat gccgcccgcc tcggtttcag caccgaggag gtcgagttcc
    16081 tcgatgacct gatcatcgaa aacaatcagt gttatcggca ggccgagatg tactccaaca
    16141 tcctggccag cctgatggat gcccgggcct cgatcgtcag caacaacatc aacgtgttga
    16201 tgaagacgct gaacatcatc accattgcca tcatggtgcc gacgttcgtg gtcagcgctt
    16261 tttccatgaa tgtcgccatc ccgatgcagc atctccctta tgccttcgag atcatcatgg
    16321 ggttggcact cttgtcggtc ctgttgttca tgctctgctg gcgcaggtac cggccatagc
    16381 tgcgtttctg taccgacgaa aaggtccgca cgccggacgg cccggggctg ctcggcttgt
    16441 gatccttgat ttccgcaccg tcactgattg atgcagttcg catcaagcgg gacaccggga
    16501 ccggaggcac acgactcgaa ccagttcaag tccgcctgat cgacgtcgtt gtccggtatg
    16561 cctcccggac cttcgacgtc gaagcagatg cagtaggccg gttcgtccgg cacagttccc
    16621 cccg
//
LOCUS       DAOTPW010000122        16599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3463_length_16599_cov_4.969778, whole genome shotgun sequence.
ACCESSION   DAOTPW010000122 DAOTPW010000000
VERSION     DAOTPW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16599
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3463_length_16599_cov_4.969778"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..508)
                     /locus_tag="P5316_12180"
     CDS             complement(149..508)
                     /locus_tag="P5316_12180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRR85756.1"
                     /translation="MSSLPASSETLRITVEQRGDCAVVKLAGSATMDVSGDLQHRLSE
                     LADLPIKRLVIDLSDLDFISSVGLGAIISAQQRCRRRDSEVQLVGPQPRILELLEVAG
                     VTRLFGVYKTLDEALAV"
     gene            complement(508..927)
                     /locus_tag="P5316_12185"
     CDS             complement(508..927)
                     /locus_tag="P5316_12185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR85757.1"
                     /translation="MPSHSEALPFRIIIPNNLTAARKVEDRIIRQVEAMGYAPRCVFG
                     IRLALEEAMVNAFKHGNRGDETKRIIVSYDVGPERVIVRVRDEGSGFDPSRVPDPTSP
                     DRVCLPCGRGIMLMRSYLDEVTFSDEGNEVQLVKERV"
     gene            complement(945..1304)
                     /locus_tag="P5316_12190"
     CDS             complement(945..1304)
                     /locus_tag="P5316_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRR85758.1"
                     /translation="MTQTLDELAVSRQDGVVLVRFRDRKIIDELQIQRIGERLQELAL
                     AERPPKMVLDFAEVEYLSSRALSELINLNRQVDQRSGQLVLAGIRPPIFEVFKITRLN
                     KLFRIEDTVADAIKLVI"
     gene            1611..2513
                     /locus_tag="P5316_12195"
     CDS             1611..2513
                     /locus_tag="P5316_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRR85759.1"
                     /translation="MSRSRIVVGVILIVLLMNATPALAWGPATHMKLGSDLLSQLGLL
                     PAAVAALLARYRRDYLFGNLAADVVIAKRLSRIKQFCHHWATGFAILEDASTDASRAF
                     ALGYLSHLAADTVAHNKFIPRQMTLTRSTLSFGHLYWELRADSTIGPHHWGQLRVVLG
                     EAFAEHERNLHSRFTDTFLPFSINWRLFYRLNRFMSRRLCRRALDRMYDMSRWTLSDQ
                     LMREYRMDCLQRMIDVVSNQRGATVLHEDPNGNIALAYSKAQRRQLRQMARCGLITPH
                     IMYEAAVSHAPMASSGAHRRLVER"
     gene            2597..4351
                     /locus_tag="P5316_12200"
     CDS             2597..4351
                     /locus_tag="P5316_12200"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="silent information regulator protein Sir2"
                     /protein_id="HRR85760.1"
                     /translation="MARYCQESIMVRRSGIAGIAVVLFVCAAAAAEERLDRGLVALKT
                     ADGRVYLGWRLRADDPRDAAFNVYRHGSNDPTATRLTASPIGDCTNYVDGNPPADAKY
                     FIRPVSAGTEGEPSPAVAVTDTSGAASCVRIKLQGNYGFQKCALADLDGDGRLDYIIK
                     QPDFNTDPYQHPGYWKRSEDTYKIEAYNHDGRFMWRYDMGWSIEEGVWYSPYLAYDLD
                     GDGRAEIYTKAGEGDPRDEKGLVTSGPEWLVKIDGLTGKVTRRLPWPDRSGYAGYNYW
                     SRNMLAVAYLDGRQPALIVQRGTYDVIKVEAYDPELRLIWRWDSRSEKERYNGSGSHG
                     TRAADVDGDGRDELILGSSVLDDNGRGLWVNPMSHPRSSHPDVCSVGDIDPDRPGLEI
                     FYGYEVDQPRNGVCLVDAATGRILWGFEGPTYHVHGFGMVADIIAAHPGQECYAGEKE
                     HGQYFLYTAKGERIGKEKINSLSPSAIWWDDDAQKELILSGRIQQYRGQVYESVQGSP
                     VAIADCLGDWREEILTSVPGELRIYTTTIPTNVRRTCLMQDRQYRLGVAAVSMGYFYW
                     PQLSLAGAARFAEARKAR"
     gene            4427..5371
                     /locus_tag="P5316_12205"
     CDS             4427..5371
                     /locus_tag="P5316_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR85761.1"
                     /translation="MPIYDVHTHVGLDLGFFLRGWRPYAAWVPDLIEHMNAHGIDRAV
                     CFPFTLPSAFDAVAFADQGRVELLPGRVPFDRENDILVREIEALEVGDRLYPLAMIDP
                     SRRVNEQLKRLERLAGRISGLKLQSTIIESPVCSLLTTGRSLLEFAADRRWPVLLHAT
                     IWEQAPHARIADCFAVAEAFPSIRFDLAHSLAFHAGYLRRAAQMPNVWVDCAALLAHC
                     RLARDNSPIIAAPADRLDADYTRPGQVLEAIHAVLGDRFLWGSDNPFMSWCDDRLRLL
                     YSYAQEAAVLKTLPEHVRADICTVGPEAWLFGRKGSTL"
     gene            5368..6156
                     /locus_tag="P5316_12210"
     CDS             5368..6156
                     /locus_tag="P5316_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR85762.1"
                     /translation="MITLKRHAALVTGSTKGVGRAIAIAFAEAGADVVIHGRTMSDEA
                     RETMEQCRRHAVRVAFVAGDLSGPGEAAVNKVFADATAAHDGIDILVNNAGQYFDVPF
                     LEMDIERFERTMRLNVASGYFLTQRFARRWIERKTAGRVLFIGSINGRLAEPLSTAYD
                     TSKGAVEMMVRTLAVELAPRGIRVNGLAPGLVRSASTAWLDSRPAKAAWIASHTPDRQ
                     IPDASVCGPGAVYLVSDAAQHVHGHMLLIDGGMSALQQPEPPAT"
     gene            complement(6206..7441)
                     /locus_tag="P5316_12215"
     CDS             complement(6206..7441)
                     /locus_tag="P5316_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRR85763.1"
                     /translation="MSVVRGNAVVGQAGGPTAVINQSLVGIIEESQKHSHIDRLLGAR
                     HGVRGIINEQFYDLNRQPTNMLELVAKTPAAALGATRDKPDEAYCEQIFNVFRKLDVR
                     YFFYIGGNDSASTAAIINRISQDAKYELRVFHVPKTIDNDLLVTDHCPGYGSAAKFVA
                     SAFIGDDLDNRALQGVKINIIMGRNAGFLTASSVLARMRPDDGPHLVYLPERAFDLDQ
                     FVRDVSAVYEQFGRCVVAVSEGIVDTQGKLWSQKVLEMVKQSAPTYDPHGNVEISAGA
                     GMLADYLASTVRSRCKIKRVRADTFGYLQRSFPGVYSEVDAWEARLVGQMAVSYSLQS
                     EDRDGSVALVRLPGTTYAVGTKLVGLKDVAPDSPPFTKVVPDEYISKEGNNVLNTFRE
                     YVGPLVGDLPKPGWLEQIG"
     gene            complement(7537..8874)
                     /gene="accC"
                     /locus_tag="P5316_12220"
     CDS             complement(7537..8874)
                     /gene="accC"
                     /locus_tag="P5316_12220"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRR85764.1"
                     /translation="MFSRIMIANRGEIALRIIRACKELGIQTVCVFSEEDRNLSYLKL
                     ADRAICIGPGAAAESYLKIDRIIAAAEVANVDAIHPGYGFLAENPQFADACRDSKIEF
                     IGPSSESMRLLGNKIEAKRLAKKAKVPNIPWTEGPVDSDDDAAKWASQVGYPVMIKAA
                     AGGGGRGIRLVHNEATLRHSLPAARSEAQASFKDPSVYLEKAVEDARHVEVQVMGDRA
                     GNVLHFYERDCSLQRRHQKLVEESPCPAINDRIREELCKAAVRLARAAKYYSAGTVEF
                     LVTPDKKFYLLEMNTRIQVEHPVSEMVTGHDLVQLQIRVAAGETLSLKQRDITHKGVA
                     IECRINAEDPAADFRPCPGKIEVFRPPGGFGVRLDTHAHAGMTISPRYDSMIAKLIVH
                     KATRAEAIACMRRCLEEFAIEPVKTTIPIHREIFSHAQFLKGTVDTGFIERTW"
     gene            complement(8903..9370)
                     /gene="accB"
                     /locus_tag="P5316_12225"
     CDS             complement(8903..9370)
                     /gene="accB"
                     /locus_tag="P5316_12225"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRR85765.1"
                     /translation="MIDVQQVRELIQLMVENDLSEIRVRQGETAISLRKAPAGVPLAG
                     APAMAAVPATLPVLQPAQAPVPPAIEKPTEDGLVPVLSPMVGTYYQAPDPDSEPYVRV
                     GMEIGPDTPICIIEAMKVFNEIKAEVAGVIERILVQDQQAVEFGQPLMLVRPR"
     gene            complement(9445..10569)
                     /locus_tag="P5316_12230"
     CDS             complement(9445..10569)
                     /locus_tag="P5316_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRR85766.1"
                     /translation="MANGRRCPKPPVAINRRIECCREQIRRRGMDGFLVTNRVDQFYL
                     TEFDGEDGAALVLPRRVYLLTDGRFAEEAGKKAPWATAVIRTGSLKDALARLTRRHRM
                     RKLGFQPEHLTVSDFGAFRKAVAPTKLRGLGRVASALRLKKDETEVAAIREAIRIAEA
                     AFRTVIGRMRLGMTERQIAAELLFEMTRLGARGASFPIIVAEGPNSSLPHAKPGERRI
                     GVGSAVLIDWGAIAEGYCSDLTRVVFVRRIPPRFRRMYEKVLAAQLEGIRAIAPGVRM
                     CDVDGKARKVLAKAGMGRRFTHGLGHGLGLDIHEAPRLARKVTDRLEPGMVVTVEPGV
                     YYPGIGGVRIEDDVLVTETGAEVLTSLPKNLDAMVVQARP"
     gene            10787..12406
                     /locus_tag="P5316_12235"
     CDS             10787..12406
                     /locus_tag="P5316_12235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRR85767.1"
                     /translation="MRIVIKEGDSFLGDVSFTEDDEVTIGSQPDCSIYLPDARVSALN
                     VLIMPTEEGHWLVENVDPGNRVLLNAHVLTQRTALQNDDEITIHDYVLKVYLEEDLNH
                     AVVQEPELTAEELIRIREFPLPAGSLVRRHSDSLSLTRLQLDRLAGACAVIDGTRDLH
                     DWVETTLNLLLDVFEARCAWVGFRRQSRGELDVQGGKLANGQPCDLNPIIELLRYRCV
                     DRHQHICIRKVREHEYIGSAMGAPLTGKTGSLGMIYVDRRVKTSRFRSSDLDMLSALA
                     ARLSLKLQALLEGHAQRSAEISATEISVVHAIQEHLDPKGVPPLEGLKLAAYSRSGQE
                     NPGDVYDVMKHPDTPLTALLLGHVNATGALLALSMARLHSTFRVGFLHNDPPHALARA
                     LNWLMYDERDPSTVDAVFLTIYPGGRVRYSRAGKIGAFVVGADGQPRPLGAADAPAIG
                     QVRNFEYQSFVEQLAPDETLVLYTRGIATCTDGQGRRFGEHRFITLVCDGFGQPPTTT
                     LQDVTTELISFFKDGRHPDDISIVLLHREED"
     gene            complement(12632..15766)
                     /locus_tag="P5316_12240"
     CDS             complement(12632..15766)
                     /locus_tag="P5316_12240"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HRR85768.1"
                     /translation="MRTGSILLIGFLVVLPATSMAGESLVNGSFEDGTLNGWTILSDS
                     VANYHNPPIKQCICGGDVTPENPGAHRFHDLLLAPDGQYFAGLTWMQDAEPYDNLPHP
                     GKLSRYLQTIDVANWNPGADRTNYHFEIWSQMAHSWHWNRHGQARQTYIIHWNNDGLA
                     PRDTALVMISGDIYDSDTIEIDTGGANRVYEFDTDGQIRPTSNVAVVVPNPLATVVIS
                     GGILDGDTIELDTTGVNHVYEFDADGSVGAGHVVVDMSSAVLKATIRIVGNVADGDTI
                     EIDPTGVNIVYEFDNNGVVAEGNVPVNGVGTYKTALNALSAKINSDPNRPYNASGRDG
                     NNVYLTWIAPGGGTTNTNLNDANGTISVTNWGRDKDVAKSRLINAINTDPTGPPFTAI
                     DGGTNTIKLIWTGAAGQAATNTNLNDANNVISITDFKGIAKNDAKAALMAAINADPGA
                     LCTASDVNSTGDDQTRYIKLTWNTLSSGGATNSNLNDTFGRISVTNFSYFQGVCDNTY
                     VIGDLDPMAFVKGSSLEFNNWRKIKLSGSIPDNPQAVVLEIRLEHEGNMSTTHNCFDG
                     VVFSAESATGSAIGPFTGDIVNSDFELEPYNTQHYGFDKNVTAYPFLVTPHPFGWDAL
                     TWRWGGSGDGAGPIEGLTNGVTVPTNPLDLSTPSGEHFWGRANDPPLGTYNSNCEGTW
                     GKLLPVRNWSPCATGIRWKLHYLTKIWNTHDMDPDWNSSYTLTLWWDIDDDARQYSIM
                     DIPPNPSTDIRHHLEHGSFPWVKIVDMNDRAYPDARTRGLIEVEQEGEFAPTDRYGNS
                     YAPEHVILRDSQWVWGWWDGTMPYRIAYDKVDFYVEAIVPTEVRIVTPPPLPAGDCDN
                     LYSVQLLGCYEPPLTFSIVSGSLPPGLTLSPTGLISGRPNTAGTFAFTVQLEDGTSAT
                     TTKGFEIAVSGNCCGPISGDLDRDSDVDQRDFALLQLCYTGSDGGVPLPPEAPDCTCA
                     DLDKDGLDIDQSDLLVFEQCASGPGIPADPQCSTEACCLTDGSCVEMLPMTCSSLGGI
                     PQGSGTTCKTASCP"
BASE COUNT         3068 a         5110 c         5035 g         3386 t
ORIGIN      
        1 cagtccctca aacgggcgat gcgtccccac cggtttcatc gtcgtgatgc ttgctcagat
       61 tgagatctca gacggcagag gcggaaggcg aaggtccgaa tagcactacg cgggccgctc
      121 tccagcccct tcggaagagc agcgaacatc acaccgcgag ggcttcatcc aaagtcttgt
      181 agacgccgaa gagccgcgtc acgccggcca cttcaagcag ttcgagtatt cgcggctgcg
      241 gtccgaccag ttgaacctct gaatcgcgcc ggcggcatcg ttgctgcgcc gatatgatgg
      301 cgcccagacc caccgagctg atgaagtcaa ggtccgacag atcgattacc aggcgtttga
      361 tggggaggtc ggcgagttcg gagagtcgat gctgcaaatc gcctgatacg tccatcgtgg
      421 ccgagccggc gagcttgacg acggcgcaat ctcctcgctg ttcgaccgtg attcgaagcg
      481 tttcgcttga agcaggaagg cttgacatca gactctctcc ttgaccagtt gaacctcgtt
      541 cccctcatcg ctgaaagtga cttcgtcgag ataggatcgc atgagcatga tcccacggcc
      601 gcatggcaga cacacgcgat cgggtgaggt cggatccggg accctggacg gatcgaaacc
      661 ggagccctca tcgcgcacac gcacgatcac tcgctccgga ccgacgtcgt agctcacgat
      721 gatgcgcttg gtctcatcgc cgcggttgcc gtgcttgaag gcgttgacca tcgcctcttc
      781 gagggccagt cggatgccga aaacgcatcg cggggcgtaa cccatggctt ccacctggcg
      841 aatgatccgg tcctcgacct tccgcgcggc ggtaaggttg ttgggaatga tgattcggaa
      901 aggcaatgcc tcggaatgcg acggcatgtc ggttgcgccc cgtctcaaat cacaagcttg
      961 atcgcgtccg ctactgtgtc ctcgattcgg aacagcttgt tgagccgcgt gatcttgaaa
     1021 acctcaaaga tcggcgggcg gatgccggcc aggacaagtt gcccgcttcg ctggtccacc
     1081 tgacgattca gattgatcag ctcgctcagg gcccggctgg acagatactc gacctcagca
     1141 aagtcaagga ccattttcgg ggggcgttcg gccagtgcca gttcctgaag ccgctcgcca
     1201 atccgctgga tctgcaactc gtcgatgatc ttgcggtcgc ggaaccggac caggacaaca
     1261 ccgtcttgcc gggatactgc gagttcgtct aacgtctgcg tcatcttcat ttactcccgg
     1321 atcggccaga gtaagcgctc acattcactc caccttggat tagagtcggc ctccgccgga
     1381 aagtcaacga accgtatctc ttttatttat aggtacttcg aaagcctcgg ggcatgacct
     1441 ccccttgaag ggcggcggcc ttcagcaacc gagtcgactt ccgtgcttct cccgcaagcg
     1501 gcttgcgcga gtctggcttc cggacccgcc cctttctatc attgttgaat gtgcgagccc
     1561 gttcgaccgc catgtcaggt cgacggatgc ccgtgttgcc ggggtgccgt gtgtcacgtt
     1621 cgcggattgt tgttggagtc atcctgattg tcctgctgat gaacgccacg ccggcgctgg
     1681 catggggacc ggcgacccac atgaagctgg ggagcgacct gctgagtcaa ctgggtttgc
     1741 ttcccgcggc cgtcgccgct ttgcttgcca ggtatcggcg agactacctc ttcgggaatc
     1801 tggctgctga tgtcgtcatc gccaaacgcc tcagccggat caagcagttc tgccaccatt
     1861 gggccaccgg ctttgcgata ctcgaggatg cctccacgga tgcgagccgg gcgtttgccc
     1921 tgggttatct ttcgcatctg gcggcggaca cggtcgccca caacaagttc attcctcgtc
     1981 agatgacgct gactcgctcc acgctgtcgt ttggccacct ctattgggag cttcgcgccg
     2041 attcgacgat cggcccgcac cattggggcc aactgcgggt ggttctcggc gaggcgtttg
     2101 ccgagcacga gcgcaacctc cattcgaggt ttaccgacac tttcctgccg ttttccatca
     2161 actggcggct cttttatcgg ttgaatcgtt tcatgagccg tcggctttgc cggcgcgcct
     2221 tggaccggat gtacgatatg tcccgatgga ccttgtcgga ccagttgatg cgcgaatacc
     2281 ggatggattg tctgcagcga atgattgacg tcgtctccaa ccagaggggg gcgactgtgc
     2341 tgcacgaaga cccgaacgga aacatcgcct tggcctatag caaggcccag cgccgccagc
     2401 ttcgccagat ggcccgctgc gggttgatca cgccgcacat catgtatgaa gccgccgtca
     2461 gccacgcgcc gatggcaagc agtggcgctc atcgccggtt ggttgagcgg tgaccccggg
     2521 atggcctgcc tcgcagtcgg cgctctcgat ccgttcagtg tcccagggcg gcactccgct
     2581 gagaagattg ctcgtaatgg cgcgctactg tcaggagtcg atcatggttc gacgttccgg
     2641 gatcgcggga atagctgttg ttttgtttgt atgtgccgca gccgcggcgg aggagagact
     2701 cgaccgtggc ttggtggcgc tgaaaacggc tgacgggcgg gtctatctcg gctggcgatt
     2761 gcgggccgac gatccgaggg acgctgcctt caacgtctat cggcacggca gcaacgaccc
     2821 gacggcgacc aggctgaccg cctcacctat cggcgactgc accaactacg ttgacggcaa
     2881 ccccccggcc gatgcgaagt acttcatacg accggtcagc gcaggcaccg agggtgagcc
     2941 ctcccccgca gtcgcggtca cggatacgtc cggcgcggcg tcctgcgtac gcatcaaact
     3001 gcagggcaac tatggttttc agaaatgcgc tctggccgat ctcgacggcg acgggcggct
     3061 ggactacatc atcaagcagc ccgatttcaa taccgacccg taccagcacc cgggatactg
     3121 gaagcggagc gaagacacct acaagatcga ggcctacaac cacgacggac ggttcatgtg
     3181 gcgctatgac atgggctggt cgatcgagga aggcgtgtgg tactctcctt atctcgctta
     3241 cgacctcgac ggtgacggca gggcggagat ctacaccaag gccggcgagg gcgacccccg
     3301 cgacgagaaa ggcctggtca ccagcggccc cgagtggctg gtcaagatcg acggcctgac
     3361 cggcaaggtc acgcgcagac tgccctggcc tgaccgcagc ggatacgccg gctacaacta
     3421 ctggagccgc aacatgctgg cggtggccta tctggacggg aggcagccgg cgctcatcgt
     3481 gcagcgcggt acctacgacg tgatcaaggt tgaggcctac gatcccgagc tgcggcttat
     3541 ctggcgctgg gactcacgca gcgaaaagga aagatataac ggcagcggct cacacggcac
     3601 ccgggctgcg gacgtcgatg gcgacggacg cgatgaactg atcctcggtt cgtcagtcct
     3661 cgatgacaac ggccggggcc tctgggtcaa cccgatgagt caccccagaa gctcgcaccc
     3721 cgacgtctgc tccgttggtg atattgatcc ggaccgaccc gggctcgaga tcttctacgg
     3781 ctacgaggtt gaccagccca ggaacggcgt ctgcctggtc gatgcggcca ccgggcgcat
     3841 tctgtggggg ttcgagggac caacctacca cgtacacggc tttgggatgg tcgcggatat
     3901 catcgccgca cacccgggcc aggagtgtta cgcaggcgaa aaggagcacg gccagtattt
     3961 tctttacacc gccaagggcg agcggatcgg gaaggagaaa atcaacagcc tttctcccag
     4021 cgccatctgg tgggacgacg atgcccaaaa agagctgatc ctctccggca gaatccagca
     4081 gtaccgcggg caggtctacg agtccgtcca aggttcgccg gttgccatcg ctgattgcct
     4141 cggtgactgg cgggaggaga ttctcaccag cgtcccgggc gaattgcgaa tctacacgac
     4201 cacgattccg accaacgtcc gccgcacttg cctgatgcag gatcgccagt acaggctcgg
     4261 cgtggctgcc gtctccatgg gatatttcta ctggccgcag ttgtctcttg ccggtgcggc
     4321 ccgattcgcc gaagcccgaa aagcacgatg atccgccttc gccgactcga cgccggcgct
     4381 tgacgacgag accggaactt ctacattacc gccgggagcg atcatcatgc ccatttacga
     4441 cgttcacacc cacgtcggcc tcgatctcgg gttttttctt cgagggtgga gaccctatgc
     4501 cgcttgggtg ccggatctca tcgagcacat gaacgcccac gggatcgacc gggcggtctg
     4561 cttcccgttc acccttccgt cggcgttcga tgccgtcgct ttcgcggatc aggggcgggt
     4621 ggaattgttg cccggccgcg tgccgttcga tcgcgagaac gacatccttg tccgcgagat
     4681 cgaggccctt gaggtcggcg accgtctgta cccgctggcg atgatcgacc cttcacgccg
     4741 cgtgaacgag caactgaaga ggctggagcg cctcgcgggg cgcatatcgg ggctcaagct
     4801 gcaatcgaca atcatcgagt cgccggtgtg cagtctgctc acgaccggcc gcagtctcct
     4861 ggaattcgcc gccgatcggc gatggccggt ccttcttcac gccacaatct gggagcaggc
     4921 tccgcacgca aggattgccg attgctttgc cgtggcggag gcgtttccgt cgatccgctt
     4981 cgatctggct cactcgctgg ccttccatgc gggttatctc cggcgcgcgg cgcagatgcc
     5041 gaatgtctgg gtggactgcg ctgctctgtt ggcacactgc cgactggccc gcgacaactc
     5101 gccgattatt gccgccccgg ccgacaggtt ggacgccgat tacacgcggc ccgggcaggt
     5161 gcttgaggcg attcacgcgg ttctcggcga ccgtttcctg tggggctccg acaatccgtt
     5221 catgagctgg tgcgatgatc gcctgcggct gttgtactcg tatgcccagg aggccgcggt
     5281 gctcaaaacg ttgcctgaac acgtccgcgc agacatctgc accgttggtc cggaagcctg
     5341 gttgttcggg cggaaaggaa gcacgctgtg atcaccttga aaaggcacgc ggcgctggtg
     5401 accggctcga ccaagggtgt cggtcgggcg attgcgatcg cattcgccga ggcgggcgcc
     5461 gacgtggtca tccacggtcg gacgatgagc gacgaggccc gcgagacgat ggaacaatgt
     5521 cgtcggcacg cggtgcgcgt cgcgttcgtc gcgggagatc tctcagggcc tggcgaagcg
     5581 gcggtaaaca aggtgttcgc cgatgcgacg gcggctcatg acgggatcga tatcctggtc
     5641 aacaatgccg gccagtactt cgacgtgccg tttctggaga tggacattga gcggttcgag
     5701 agaacgatgc gactcaatgt ggcgtcgggc tattttctga cgcaacggtt cgccaggcga
     5761 tggatcgagc gaaagactgc cggacgcgtg ctcttcatcg gttcgatcaa cggccgcctg
     5821 gctgagccgc tttcgacggc ctacgacacc tccaagggcg cggtcgagat gatggtccga
     5881 acgctggccg tcgagctggc accccgcggc atccgagtga acggattggc tcccggtctg
     5941 gtgcgatcag cctcgacggc ttggctggac agccggccgg ccaaggcggc atggatcgcc
     6001 tcgcataccc ccgacaggca gatacctgac gcgtccgtct gcgggccggg cgctgtctac
     6061 ctggtcagcg atgcggccca acacgtccac ggccatatgc tgctcatcga tgggggcatg
     6121 agcgccctgc aacaacctga accgccggca acttgacgcc ctcccatcgg gggccgcaaa
     6181 tcgtccagga gattcctgcg ggccctcagc cgatttgttc caaccagccc ggttttggca
     6241 ggtcgccgac gagtgggccg acgtactcgc ggaaggtgtt gagcacgtta ttgccttctt
     6301 tgctgatgta ctcgtccggg acgaccttgg tgaacggggg actgtccggg gccacgtcct
     6361 tgagcccgac cagcttggtc cccacggcat aggttgtgcc cggcaaacga accagggcca
     6421 cgctgccgtc gcgatcctcg gattgcagcg agtagctcac cgccatttgt ccgaccagac
     6481 gcgcctccca tgcgtccacc tcgctgtaaa caccggggaa gctccgctgg aggtaaccga
     6541 atgtgtcggc ccggacgcgc ttgatcttgc accggctccg gaccgtgctc gcgaggtaat
     6601 cggcaagcat gccggccccc gccgagatct ccacgttgcc atgcgggtcg taggtgggag
     6661 cactctgctt gaccatctcc aggactttct gcgaccatag cttgccctgc gtatcgacaa
     6721 tgccctccga gacggcgacc acgcaccgtc cgaactgctc atacaccgcc gagacgtctc
     6781 gcacgaactg atccagatcg aacgcacgct ccggcaggta taccaggtgc ggaccgtcat
     6841 cgggacgcat cctcgccagg acgctcgacg ccgtgaggaa tcccgcgttt cggcccatga
     6901 tgatgttgat cttgacgccc tgaagggcac ggttgtccag atcgtcgccg ataaatgccg
     6961 atgcgacgaa cttggcggcg ctcccgtaac ccgggcagtg gtcggtgacc agcaagtcgt
     7021 tgtcgatcgt cttggggacg tggaaaaccc gcagctcgta ctttgcgtct tgggagatac
     7081 ggttgatgat cgcggcggtc gaagccgaat cgtttccgcc gatgtagaag aagtaccgca
     7141 cgtccagctt gcggaacacg ttgaagatct gctcgcagta tgcttcatcc ggcttgtcgc
     7201 gggtcgcccc cagcgcggcg gccggcgtct tggccaccag ctccagcatg ttcgtcggct
     7261 ggcggttgag gtcgtaaaac tgctcgttga tgatgccgcg cacgccgtgc cgggcgccca
     7321 gaagccggtc gatgtgagag tgtttctggc tctcttcgat gatgccgacc aacgactgat
     7381 tgatgacggc ggtgggaccg cccgcctggc ccacaacagc attaccccgc actacgctca
     7441 tgttcgctat acctcgtttg cgattgccct gattcctctg cttcattagg gtgttcaaaa
     7501 atctgccggg cactcatccg gcagcggggg gagattctac cacgtgcgct cgatgaatcc
     7561 agtgtccacg gttcctttca ggaattgagc atgactaaag atctcgcggt gaatcggaat
     7621 tgtcgtcttg accggttcaa tggcgaactc ttccaggcag cgtcgcatgc aggcgatcgc
     7681 ctcggcacgc gtcgccttgt ggacaatcag cttggcgatc atcgaatcat atcgcgggct
     7741 gatggtcatg cccgcatgag cgtgagtatc cagtcgcacg ccgaaaccgc cgggggggcg
     7801 gaagacctcg atttttccgg ggcaggggcg aaagtcggcg gcggggtcct cggcgttgat
     7861 ccggcactcg atggccacgc ccttgtgcgt gatgtccctc tgtttgagcg agagcgtttc
     7921 gcccgccgca acgcgaatct ggagctgcac caggtcgtgg cccgtgacca tctcgcttac
     7981 cgggtgttca acctgaattc gagtgttcat ttcgagcaga taaaactttt tatcgggcgt
     8041 aacgaggaac tcaaccgtac ccgcactgta gtacttggcc gcccgggcca gccggacggc
     8101 tgccttgcac aactcctcgc gaatccgatc gttgatcgcc ggacagggcg attcctccac
     8161 cagcttctgg tgtctccgtt gcaggctgca gtcccgctcg tagaagtgca ggacgttgcc
     8221 ggcccggtca cccatgacct gaacctcgac gtggcgggcg tcctcgacgg ctttttcgag
     8281 gtagacggac ggatccttga acgaagcctg agcctccgaa cgggcggccg gtagcgaatg
     8341 acgcagcgtg gcctcgttat ggaccagccg aatgccccgc ccgccgcccc cggccgcagc
     8401 cttgatcatg accgggtagc cgacctgtga agcccacttc gccgcgtcgt catcgctgtc
     8461 cacaggcccc tcggtccagg gaatgttggg caccttcgcc ttcttggcaa gtctcttggc
     8521 ctcgatcttg tttcccagga gccgcatgga ttcggagctc gggccgatga actcgatctt
     8581 ggagtcacgg caggcatccg cgaactgcgg attctcagcc aggaatccgt agcccgggtg
     8641 gatcgcgtcc acatttgcca cctccgccgc cgcgatgatc cggtcgatct tcaagtagct
     8701 ctcggccgcc gcgccggggc cgatgcatat ggctcgatct gccagtttca aatacgagag
     8761 gttcctgtcc tcttccgaaa aaacgcagac ggtctggatg cccagctcct tgcaggctcg
     8821 aatgattctc agggcgatct cgccccggtt ggctatcatg atccgtgaaa acatgcttgc
     8881 ttccgttgcc gggcgatgaa gactatctcg gccgtaccag catcagcggc tgaccaaact
     8941 ccacggcctg ctgatcttgg acaaggattc tctcgatgac cccggctacc tccgccttga
     9001 tctcgttgaa gactttcatc gcctcaatga tgcagatggg ggtatccgga ccgatttcca
     9061 tgcccaccct gacgtaaggt tcggagtccg gatccggggc ctgatagtag gtgcccacca
     9121 tcggcgagag gaccggaacc agtccgtctt ccgtcggttt ctcgattgcc ggtggcacgg
     9181 gggcttgagc gggctgaagg accggcagtg ttgcaggcac ggccgccata gccggggcac
     9241 cggcgagtgg gactccggcc ggagccttgc gaagactgat ggccgtctcg ccttgcctga
     9301 cgcggatttc gctgaggtcg ttctcgacca tcagttggat gagctcgcga acttgttgaa
     9361 cgtcgatcac accacactcc tacctgcctt ggccattgcg ctgcccgtca ggcgggttcc
     9421 agggccgcag agccattcgg aacctcaagg gcgcgcctgg accaccatgg catccaggtt
     9481 cttgggcagg ctggtcagta cttcggcccc ggtttccgtt acaagaacgt cgtcttcgat
     9541 ccgaaccccc ccgattccgg ggtaatagac gccgggttca acggtcacaa ccatgccggg
     9601 ctccagtcgg tcggtgacct ttctggccag gcggggcgct tcgtggatgt ccaatccaag
     9661 tccatggccc agcccatgag tgaaacggcg gcccatgccg gccttggcca agactttgcg
     9721 ggccttgcca tccacgtcgc acatccggac gccgggggcg atggcccgga taccctccaa
     9781 ctgggctgcc agaacctttt cgtacatccg ccgaaagcga ggcgggattc tacggacgaa
     9841 cacgactcgg gtcaagtcgc tacagtaccc ctccgcgatc gccccccagt caatcagaac
     9901 cgcactgccc accccgattc ttcgctcgcc cggtttggcg tgaggcagtg atgagttggg
     9961 tccctcagcc acgatgattg gaaaactggc gccacgggct cccagtcggg tcatctcaaa
    10021 cagcagttca gcggcgatct ggcgttcggt cattcccaga cgcattcggc cgatcaccgt
    10081 ccggaatgct gcttccgcga ttcggattgc ctcccggatg gctgcaacct cggtttcgtc
    10141 ttttttcagt cgcagcgcgg atgcaacgcg gccaagccct cgcagcttcg tcggtgccac
    10201 ggctttgcga aaagctccga aatccgacac agtcagatgc tcgggctgaa agcccagctt
    10261 cctcatgcga tgccgtcggg tcagacgggc caaagcatcc ttcagcgagc cggtccgaat
    10321 caccgccgtt gcccagggag ccttcttgcc tgcctcctct gcgaaccgtc catcagtcag
    10381 cagatatacc cgcctgggca gaacaagggc ggccccatct tccccgtcaa attccgtgag
    10441 gtagaattgg tcaacccggt tagtgacgag gaaaccgtcc atacccctgc ggcggatctg
    10501 ctcgcggcag cattcgatgc gacggtttat cgccacgggc ggtttgggac agcggcgacc
    10561 gtttgccaat gttggcctcc tgggaccagt taccgactat tcggccggct ggaaatctac
    10621 gggccgaggc ccttggtgtc aaagatggga cggtattgaa ccattggtgc cccttgctgg
    10681 cccggccgct ttgtgtggcc gatcaatgaa aaatgcgggc caagccacgc cacggcaaag
    10741 gtgcgaaccg gaggctggac gtgtccggca gatgcgggtg tagtcgatga ggatagtgat
    10801 caaggaaggt gattcctttc tgggagacgt gagcttcacc gaagacgatg aagtcacgat
    10861 tggctcgcag ccggactgca gcatctacct gcccgatgcc cgtgttagcg cgctcaatgt
    10921 gctcatcatg cctaccgagg aaggccactg gctcgtcgaa aatgttgatc ccggtaaccg
    10981 cgtcctgttg aatgcccacg tgctgaccca acggacggcc cttcagaacg acgacgaaat
    11041 cacgattcat gactatgtac ttaaagtcta tctcgaggaa gacctgaatc atgcggtggt
    11101 ccaggaaccg gaactgacgg ccgaggagct gattcggatc cgcgaattcc cgctgccggc
    11161 cggctccttg gttcgtcgtc acagcgattc attgtctctc acccggttac aactggaccg
    11221 gctcgccgga gcctgtgccg tcatcgacgg cactcgcgac ctgcacgact gggtggaaac
    11281 cacgcttaac cttctcctcg atgtgtttga ggcccgttgt gcctgggtcg gcttccgacg
    11341 acagtctcgg ggcgagctgg atgtccaagg gggcaagctg gccaacggcc agccatgcga
    11401 tctcaaccca atcatcgagc ttctgcgata tcgctgcgtt gatcggcacc agcacatctg
    11461 cattcgcaag gttcgcgagc acgaatacat cggctcggcc atgggtgctc cgctgacggg
    11521 caaaaccggt tcgctgggca tgatctacgt tgaccgccgg gtaaagacct cgcgcttccg
    11581 cagctcagac ctcgatatgc tttccgccct tgctgcccgg ctctcgctca aactccaggc
    11641 cctgctcgaa ggacatgctc agcgctccgc cgaaatctcg gccactgaga tcagcgtggt
    11701 gcatgcaatc caggaacacc tcgatcccaa gggcgtgcct ccgctcgagg gtctcaagct
    11761 ggctgcgtac agccgctcgg gccaggaaaa ccccggcgac gtatacgacg tgatgaaaca
    11821 ccccgacacg cccctgacgg cgctgctgct cgggcacgtc aacgccaccg gcgcattgct
    11881 ggccctgtcg atggcccgcc tgcattccac ctttcgcgtc ggcttcttgc acaatgaccc
    11941 gccgcatgcc ttggccaggg ccctgaactg gctgatgtac gatgaacgcg acccctccac
    12001 cgtggacgcg gtattcctga cgatctaccc cggcggaagg gtgcgctatt cgcgtgccgg
    12061 caagatcggg gcgttcgtcg tcggagccga tggtcagccc cggccgctcg gagccgccga
    12121 tgctccggcc atcgggcagg tccgcaactt cgagtaccag tcgtttgtcg agcagctcgc
    12181 ccccgacgag acgctggttc tgtacacccg aggcattgcc acctgcaccg acggccaggg
    12241 ccgtcgattt ggcgagcacc gttttatcac cctggtctgc gacggtttcg gtcagccccc
    12301 gaccacaacc cttcaggatg tcaccaccga actgatcagc ttcttcaaag acggtcgcca
    12361 ccccgacgac atcagcatcg tgctgctgca tcgcgaagaa gactgataaa cgctctcaga
    12421 tctgttggga cgccccgtcc caaggttggt gttgttcgtc gtcgccggtt gttgaaacag
    12481 ccgctgcgtg gtaccgcgca gccggttggg gcgcgggccg ttaccaccct gtgcggtagg
    12541 gtgaggtata ccggggggaa ccatcggtga aatctgcgat gattccccca cggtctctct
    12601 ctgagcttcg ccgctccgac ccggttggct gctacggaca actcgccgtt ttgcaggttg
    12661 tcccgctgcc ttgcgggatc ccacccaggc ttgagcacgt cattgggagc atctcgacac
    12721 aacttccgtc tgtcaggcag catgcttccg tcgaacactg cggatcggcc gggattcccg
    12781 gaccgctcgc gcactgctcg aacacgagca ggtcggattg gtcgatgtcc agaccgtcct
    12841 tgtccagatc ggcgcacgtg caatccgggg cctccggtgg cagcggcacg ccgccgtccg
    12901 agccggtgta acaaagctgt agaagggcga agtctctctg gtcgacgtcg ctgtcacggt
    12961 cgagatcacc ggagatcggg ccgcaacaat tgccgctgac ggcgatctca aagcccttgg
    13021 tggtggtggc ggacgtgccg tcttcgagct gcaccgtgaa ggcaaaggta cccgccgtgt
    13081 tcggccgtcc agaaatcagc cccgttggcg acagcgtcaa accgggtggc aggcttccgg
    13141 agacaatcga gaaggtcaac ggcggctcat agcagccaag cagttgaacg ctgtacaggt
    13201 tgtcgcaatc gcctgccgga agaggcggcg gggtgacgat tcgcacctcg gtcgggacga
    13261 tcgcctcgac gtagaaatcc actttgtcgt acgcgatgcg gtacggcatc gtgccatccc
    13321 accatcccca tacccactgg ctgtcccgca ggatgacgtg ctccggcgcg tacgagttgc
    13381 cgtaacggtc ggtcggggca aactcgccct cctgttccac ctcgatcaat cctcgcgtcc
    13441 tggcgtcagg ataggcccga tcgttcatgt ccacgatctt tacccaagga aatgacccat
    13501 gttcaaggtg gtgtctgatg tcagtggagg ggtttggcgg aatatccata atcgagtatt
    13561 gccgtgcgtc atcgtcgata tcccaccaca aggtcagggt gtaactggag ttccagtccg
    13621 gatccatgtc gtgcgtgttc cagatcttgg tcagatagtg cagcttccaa cggatgcccg
    13681 ttgcgcaggg cgaccagttg cgcaccggca acagttttcc ccaggtccct tcacaattgc
    13741 tattataggt tcccaagggc ggatcgttgg cccggcccca gaaatgctcg cctgacggcg
    13801 tggacaggtc cagagggttg gtcgggacgg tcacgccgtt cgtgagtccc tcgatgggtc
    13861 cggctccgtc cccggatccg ccccaccgcc aggtgagcgc gtcccagccg aatgggtggg
    13921 gcgtaacaag gaatggatac gcggtgacgt ttttgtcaaa tccgtaatgc tgggtgttgt
    13981 acggctccag ctcgaaatca ctgttcacaa tatcgccggt gaacgggccg atcgcgcttc
    14041 ccgtggccga ctcggccgag aagaccacgc cgtcaaagca attgtgcgtg gtgctcatgt
    14101 ttccttcgtg ctccagccgg atttcaagca cgacggcctg ggggttgtcc ggaatactgc
    14161 ccgagagttt gatcttgcgc caattgttga attccagaga cgagcccttc acgaaagcca
    14221 tggggtccag gtcgccgatg acataggtgt tgtcgcacac cccctggaaa tagctgaaat
    14281 tggtcacgct gatgcggccg aacgtgtcat tcagattcga gttggtcgca ccgccggagc
    14341 tcagcgtgtt ccaggtgagt ttgatgtagc gcgtttggtc gtccccggtg ctgttgacat
    14401 cggatgcggt gcacagtgcg cccgggtcgg cattgatcgc ggccatcaag gcggccttgg
    14461 cgtcgttctt ggcaattccc ttgaaatcag tgatcgaaat gacgttgttt gcgtcgttca
    14521 gattggtgtt ggtggctgct tgcccggcgg cgccggtcca gatcagcttg atcgtattcg
    14581 tgccaccgtc gatcgcggta aagggtggcc cagtgggatc cgtgttgatg gcatttatga
    14641 gccgcgactt cgcgacatcc ttatcacggc cccaatttgt gaccgaaatg gttccattgg
    14701 catcgttgag attggtgttg gtcgttccgc cgccgggtgc aatccaggta aggtaaacgt
    14761 tgtttccgtc tcggccagac gcattgtagg ggcggttggg gtcgctgttg atcttggcgc
    14821 tcagtgcgtt caaggcggtc ttgtaagtgc caacaccgtt gaccggcacg ttcccctccg
    14881 caacaacgcc gttgttgtcg aactcgtaca caatattgac acccgtggga tcgatctcga
    14941 tcgtgtcgcc atccgcaacg tttccgacga tcctgatcgt tgctttgagc acagcactgc
    15001 tcatgtccac gacgacatga ccagctccga ctgaaccgtc ggcgtcaaac tcatagacgt
    15061 gattgacacc cgtggtgtcc agttcgatcg tgtcgccgtc caggatgccg ccggaaataa
    15121 cgacggttgc caacgggttc gggaccacca ccgcgacatt gctggttggc cgaatctgtc
    15181 cgtcagtgtc aaattcgtac acgcgattgg ccccgcccgt gtcgatctcg atcgtgtcgc
    15241 tgtcgtagat gtcccccgaa atcatcacca gggccgtgtc ccgcggcgcc agcccgtcat
    15301 tgttccagtg gatgatgtac gtttgccgcg cctgcccgtg gcggttccag tgccaactgt
    15361 gagccatctg ggaccagatc tcgaagtggt agttggtgcg gtcggcaccg ggattccaat
    15421 tggccacatc gatggtttgc aggtagcgcg aaagctttcc tgggtggggc aggttgtcat
    15481 aaggttccgc gtcttgcatc caggtcagtc cggcgaaata ctgaccatcc ggtgcaagca
    15541 gcaaatcgtg aaatctgtgt gcccccgggt tctcgggagt tacatcgccc ccgcaaatac
    15601 actgtttgat cggcgggttg tgatagttgg ccacgctgtc ggacagaatg gtccagccgt
    15661 tgagcgtgcc atcctcaaag ctgccgttga ccagggattc ccccgccatg gatgttgccg
    15721 gcagaactac gagaaaacca attagaagaa tggaacccgt tcgcatgttc atgccctccc
    15781 gaaaggcata agaaaggagt tgtgcatgcg gctctgcctg acggctttgc cgcgacggtt
    15841 caacactcag gttcgatgag aaagctgcac ttggggaagc aaggacgagc ctttcgagct
    15901 tgcattcaca cgggtgccga cttcaacatc gcccaccggc caagctgctg ataaggcgta
    15961 gcctatctca agccttcctg agcagtcaag cacaaaatgc gaaaaattct gtggactcat
    16021 aagaatttcg cggtgttcga ttccaggaga gatgagcgct ggatcgtgca tagggcgaag
    16081 accctgccgt attatgaacc aggttaatcc tatcaaaaaa acactgaagg ggctgacaaa
    16141 ggcacctgga acttcgcccg gcaacggcgg gcagcgagga ccgcgacgtc accatcctgc
    16201 cggcgttgcg gcagtacagc ggagagctgg tgacgtcagc attctggatt cttgcgatcg
    16261 cgacacagcg atgtgtgcat gctgcttaga tgatcgaaat agaacttcag gaagccctcg
    16321 gtcggcatgc ccattttcct gattgcctgg gttttctggg tgtcagcttg cggtcgagcg
    16381 gtgcttacag tacggcgagg acgtcttgac ggcccggcaa tagtccggtt cgttcatggc
    16441 cccggaactg tgctggacgt catcggggcg gattgcccgg ggacgccgaa caccgtccgg
    16501 tgccggccgt tcccccaacg ggtgacgcga tctggatagc cgcggacgga ggccatccag
    16561 ccggcagggc aagcatatcc tgcgccgtga gccggcagt
//
LOCUS       DAOTPW010000123        16586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3469_length_16586_cov_5.668744, whole genome shotgun sequence.
ACCESSION   DAOTPW010000123 DAOTPW010000000
VERSION     DAOTPW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16586
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3469_length_16586_cov_5.668744"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="P5316_12245"
     CDS             <1..1527
                     /locus_tag="P5316_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRR85769.1"
                     /translation="GGRKTDSVPFSEENGGVVYRGDTKSLTAHVDRFLDAPFNISGDE
                     LFGSLSGKVTGWVDLDPSLTGDTRLVDMGGTTGRGIDTSGLLRISLDVPAVSFTVDLS
                     NADTAQNVIDMINDAAARAGLNVGPGLDFNASYNPSLNGFQIDVGAGNVSVQDVGGGV
                     TGRDLGLVGTAAGSLVGADLQPRLVPMTTVASLFGGAGAALGSIIITNDNLTATVDLS
                     SAVTIQDILNAVGSAGVKVKAQINEAGTGLDVINLVSGLEMRIGEAGDGSGTAEVLGI
                     RSMYADTPLSRLNNGRGVEFRADHDDLLINTKDGNSFTVDLDGCLTVQDVLDRINAAA
                     GGAVTASLALTGNGIRLVDNTGGGGTFSVCRADLSPAIDGLGLEKSTTGNEIVGDDVN
                     GIEPDSVFSALIELYRALVSGGPDAEQRITSAGSRIREFIDHATRVQGRVGARSQAMR
                     TRLELTEDAVVATQALLSEVKDLDYTEAVTRFQQAQTILQANLMTGARLMQLSLMDYL
                     "
     gene            1816..2268
                     /locus_tag="P5316_12250"
     CDS             1816..2268
                     /locus_tag="P5316_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRR85770.1"
                     /translation="MMIQTSRFGPLEVDETRLIRFEKGILGFPDQHEYALIQTAEDSG
                     FYWLQAVDRAELAFVVCDPRLFVPDYVVPVKLEELTQIGLTDPSGAQVFTIVNKVDSI
                     LTGNLQGPLVVNVETRQAKQLVLSDRRYSTRHPLMNLSRKPEAVSKTA"
     gene            2503..2754
                     /gene="csrA"
                     /locus_tag="P5316_12255"
     CDS             2503..2754
                     /gene="csrA"
                     /locus_tag="P5316_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRR85771.1"
                     /translation="MLVLSRQRDQTIMIGDDIEITVVDIRGDKVRLGINAPAEIPVHR
                     KEVYEAIKRENRAAAGVKPEDIANVAGTPANHSGSSKER"
     gene            2860..4314
                     /locus_tag="P5316_12260"
     CDS             2860..4314
                     /locus_tag="P5316_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRR85772.1"
                     /translation="MARINTNVPAIVAQRALRQSQRELQVSLERLSSGLRINRGADDP
                     AGLISSELLRAEISGIQKSISNSQRAINIIATAEASLNEVASLLTDIQGLIVEIANDA
                     AMSEDEKQANQLQINSAIESITRIANTASFAGRKLLNGELDYVTSGVDPTMIGTYAIH
                     RCQFGTASYIPVDVAVTVSAQHAQLQIRNSAITNTINVEIRGSIGTTVLSFTSGTSVT
                     DIMAAVNRESQASGIEAVYLNDANPASGMAFQSIGFGSDEFVEVTLLPGSGALTLYRP
                     DGTTQSSSTMRSIGRDAIASINGAQVIGRGLELNLQTRTLELSLTLDKNFGIGSTTFA
                     VTGGGALFQLGPDVNTNQQVNLGIQSVAASRLGNPLIGYLSQVMSSQAFDPTRSLSNA
                     NYASQIVNEANRQVSVLRGRLGAFERNTLQTNIRSLEVTMENLISSESAIRDADFAYE
                     TSRLTRNQILVSAGTSVLALANSSSQNVLALLGG"
     gene            5103..6551
                     /locus_tag="P5316_12265"
     CDS             5103..6551
                     /locus_tag="P5316_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRR85773.1"
                     /translation="MSRIMTNVSSLVAARILDSNNKMLNTSLERLSTGYRINRGKDDP
                     AGLIASETLRGEIAAIKAAMDNTERADMVISVAEGGLQEISNLLVELESLVDTTANEA
                     ALGSAEVEANQLQIDSILDTINRIATSTEFQGKKLLNGTLDYTTSGVTIGPSGGSAIA
                     ALQINSARIPPGGYRTVTVQVTTSAQTARVYASGSAVAAGGVTLQVSGKYGVEQFSFG
                     SGTSFSEVANAINQSKALTGVSASFSATGGGTLMFNSTDYGSEAFVTVQTIQGTTLAV
                     SGGDSTYGDHGVDVGLTINGAQAVTKGLDASLHTTVLDLDLTLDAGFAQQTVLSKTFY
                     INGGGADFSIAPTLGLNATASLGIQNVATGNLGKGGLGYLSSLGRGQDNDMASGNFAV
                     AQRIIRQANEQISSLRGRLGAFSKNTLTSAYNALAVALENTSAAESAIRDTDFARETS
                     ELTRAQILVNAATSTLQLANAQPRNVLALLGG"
     gene            7146..8567
                     /locus_tag="P5316_12270"
     CDS             7146..8567
                     /locus_tag="P5316_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRR85774.1"
                     /translation="MRINTNVAAIATTNRLINNQDDLNIRLERLSTGLRINRGADDPA
                     GLIASEVLRSQIRGISQAVENSQRAMNVISVAEAALNEVSSLILDLRSLIVHAANEGA
                     ISQSEIEADQLQIDSILEAIDRIANTTTFAGRKLIDGSLSYTVSGLHTSAIARAMLFG
                     ARVPDYGATHVTVSVIQSAQTAQLVYAGSSIGYNVTLELQGTLGTEIVAITSGSSVEQ
                     IAANINASTVVTGVSATVSGTGANARLLLNSVEFGERAFVSVQPLEGNFIVAEGARIQ
                     DNGQDAGVLINGQVAAFDALTGLLRANGLDMEIELTVAGATTIASTDFYITGGGATFQ
                     IGPDVNPGGQISIGIPSIGTAHLGDVQNGFLNTIRGAGRNSIVGGHCAEAERVVTKAL
                     NQIAVLRGRLGSLQRNQIETNMNSQRVALENVMASESAIRDADIATEVANLTRAQILV
                     QSTSTVLGIANQLPRNVLSLLQG"
     gene            8761..11847
                     /gene="fliD"
                     /locus_tag="P5316_12275"
     CDS             8761..11847
                     /gene="fliD"
                     /locus_tag="P5316_12275"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRR85775.1"
                     /translation="MGTISTGVGLVSGLNIQDIVSQLIEIESRPIKRLEQRVTDTQAR
                     QTAYTELSALLLGAKGLIHTFVLPSAFKVKSATSSNESVLTATAKTTASTGRYSFLVK
                     SLATSHQVVSSGFADPDRTPVGAGTLIFAPSQARVDPKTAVSTLNGFNGIRRGMVRIT
                     DRSGASADVDLRAPTSVADILNAINSQTGISVKAEVDGGRIVITDTTGLSSGNLVISD
                     LSGGGAASDMGIAGTFSSGRAVGKDMLWMTATTRLTALNDGLGVRSNGLLSDLRFELK
                     GGEVFEATLSENLRFTMNLAMLNSGLGVRADETGQRVIRITNRAGAGAEIDLSSAQTL
                     QDVSNAITNAGLSLGVSISGNKLVITDRSGGTDRNLRIEDVKGYAAADLGITADTAES
                     GVTGRGIYRIETIGDVLRAIQYAEGNNGYVTAALSADGKGITITDNTTGLNDTLVSAV
                     NGSLAAYDLGLLDSRSATERSFSGSTFTGSDLLGGLNTVMLKSLKGGAGVRVGLVEFT
                     RASGEVFSLDFTSAKTLADVINIINADGRLHAEVGVNGTEIAITDTSGATGPMSAAGS
                     LAEDLGLTDRGGVLVSGNLNRQFISENTLLASLRNGKGIRYGQIRLSDSRGNSATITL
                     NEDVHRTVGDVLREINRLFVGVEAGINEDGDGIKLVDTAGGTLTMSVSDVAGGGAAAD
                     LGIKGSAQNGEITSSYRSRIEVDADDTLYDVVSKINDAKIGVQANVINDGSAWQPYRL
                     TLTSQTSGLAGQMALHTTPGMMKLNLLSDARDAVVVVGDLNSSSAVVMASGSNSISNA
                     IRGVTLNLTQTSDLPVTVNVQNDVDTVVAGINGFVEAFNLFVDKVAELTKFEAATNTR
                     GLLLGDGTTMQVRDSLYRVLLANVNDSSLKYTNVTQVGLKLMTKSGGKLQFDEEKFRQ
                     AFEEDPDAVNELFTRIVKNKEGKSVQIGIAGQLDSLLSRLTSSVDGTLTLRNKSLQDE
                     IDQYVKRQEELQERIDAKEAQLYAKFYAMETALSSLQSQQSALAGLAALVNSTKTK"
     gene            12017..12493
                     /gene="fliS"
                     /locus_tag="P5316_12280"
     CDS             12017..12493
                     /gene="fliS"
                     /locus_tag="P5316_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRR85776.1"
                     /translation="MSREPNPYLRDAILTASPEQLQLMLYDGAIRFTRQGIKAIEDKN
                     WEQCFNAFNRAQQIILEMLNALNYDVDRELCKRMAGIYNFIYRKLIEACSSRELQAAN
                     DALGLLEFQRETWVMLIERLKQERSGVNSSQAGDAQSSKVGEQTAAPSYGTLCLEG"
     gene            12758..14254
                     /locus_tag="P5316_12285"
     CDS             12758..14254
                     /locus_tag="P5316_12285"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR85777.1"
                     /translation="MMAKENPGPLGDGLLTGCFSEKAVTKAAHDRRKSPPRLTHFVER
                     VKAVSGSLFFLIAWTMLQLTPAPAQNVATTTQAEPPSGALAGLAGISEVKPGMSTKEL
                     IELARQLAQSHRWDDAEKILTEVIRQEPRNLSAYQSAAQMYEIRASVVRADASLPDAA
                     EKSNTLIDQAVKTYTEYAGPLALEMGDVATAEEIYRTVLRYERHRYNPRALLGIARVL
                     RAKGSPEAIDRYKTYVNPRICMDGAKDPQAHLELGRLLYERRYLNQAVSTLETARSLD
                     PENPEILLELARAYQETAFRPKALSVAQEAVKKSPGNPAYRSVHAQILLAQSGVQQKG
                     NAMSDEERRMFNAARAEIEEAVRLARTGLQDAPADMTRLGVLSGCLQVQQQILSIAAA
                     INPGDVRLVVDLARCRQEQSATQHALQLHGALNDLLRAPPAARGDASYLETLADLQFR
                     VHLVTDAELTCKELLTLDPNNAVAKQILEKVAGSSGISPPGPGAAPSK"
     gene            14495..15784
                     /locus_tag="P5316_12290"
     CDS             14495..15784
                     /locus_tag="P5316_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR85778.1"
                     /translation="MSDFKIQPSQRLLRLPPYLFAKINALKHKKRQEGIDIIDLGMGN
                     PTDPTPQPIVDKLCEAARDPRNQRYSVSKGVLNLRRDVARHYHERYGVQVDPETEVVA
                     CIGSKEGISHLLLAMLGPGDTAVVGDPAFPIHVYGVALAGANVISVPLGNDEAFLERI
                     RYVVQHLYPRPKLLILNYPHNPTAMTVDPPFFDKAVALAREFGVAVLHDFAYGETCFD
                     GYKAPSFLAAEGAKEVGVEFTTMSKPYNMAGWRIGFCVGNQTMCEALGTIKGYYDYGI
                     FQAVQIASIIALRHCREFVEEQAQAYQRRRDVVLEWCERLGWQAEKCRASMFVWARVK
                     DEHLAGRSSIDFAMRMMEEAEVALAPGRAFGENGEGYVRIALVENEQRLRQAMRQIHR
                     AIGKKPIKGTPLRKKRPANPGRQTQARQPSEVNIPDE"
     gene            15813..16433
                     /gene="hisH"
                     /locus_tag="P5316_12295"
     CDS             15813..16433
                     /gene="hisH"
                     /locus_tag="P5316_12295"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRR85779.1"
                     /translation="MIAIVDYGMGNLRSVQKALERVGASAAIVSAPSDVQRADKIIVP
                     GVGAFGDAIANLRQRGLVEPLVKAARSDRPFLGICLGLQLLFDVSYEEGEHQGLGIIP
                     GRVLRFDFSGRPEAAGLKVPHMGWNQVAWTGSCPLLEGLTSPQYFYFVHSYYVSPVDR
                     SVACGLCDYGGPFAAMICRGNLYATQFHPEKSQKVGLRILENFARL"
BASE COUNT         3409 a         5154 c         5049 g         2974 t
ORIGIN      
        1 ggtgggcgga agaccgattc cgtgcccttc agcgaggaaa acggaggcgt cgtctaccgc
       61 ggcgacacca agtctctgac cgcgcatgtc gatcgcttcc tggatgcccc gttcaacatt
      121 tccggcgacg aactgttcgg ctcgctcagc ggcaaggtaa ccggttgggt ggaccttgat
      181 ccgtccctga ccggcgacac ccgactggtc gacatgggcg gcacgacggg caggggaatc
      241 gacaccagcg ggctgctgcg catctcgctg gacgtcccgg cggtcagctt caccgtggat
      301 ctgagtaacg ccgacaccgc ccagaatgta atcgatatga ttaacgacgc cgcagcacgg
      361 gctgggttga atgtaggtcc gggcttggac ttcaatgctt cgtacaaccc gagcctcaac
      421 ggcttccaga tcgacgtcgg cgcgggaaac gtgagtgtgc aggatgtcgg cggcggcgta
      481 acgggccgtg acctgggcct tgtcggtacc gcggccggaa gcctcgtcgg ggccgacctc
      541 caacccagac tcgttcccat gactaccgtc gcgtcgctgt tcggcggagc aggggctgct
      601 ttgggatcaa tcatcatcac gaatgataat ctcactgcaa ccgtcgacct gagctcggct
      661 gtcaccattc aggacatcct caacgccgtc ggcagcgccg gcgtcaaagt caaggctcag
      721 attaatgagg ctggcacggg tcttgacgtc atcaatctgg tctccgggtt ggagatgcgc
      781 atcggcgagg ccggcgacgg ctcgggcacc gccgaggtcc tcggcattcg ctcgatgtac
      841 gccgacacgc cgttgagccg gttgaacaac ggtcggggcg tggagttccg agcggatcac
      901 gacgacctgc tgattaatac caaggatgga aacagcttca ccgtggatct cgacggctgt
      961 ctcaccgttc aggacgtcct tgaccgaatc aacgccgcgg cgggcggggc ggtcacggct
     1021 tcgcttgccc tgaccggaaa cggaatccgg ctggtggata acaccggcgg cggggggacg
     1081 ttcagcgtct gccgtgccga tctgtccccg gccattgacg gactgggcct ggagaagagc
     1141 acgacgggga acgaaatcgt cggcgatgac gtgaacggga tcgagcccga cagcgtgttc
     1201 agcgccttga tcgagctgta ccgggccctc gtgtcgggcg ggcccgatgc cgagcagcga
     1261 atcacaagcg ccggttcccg gattcgcgaa ttcatcgatc acgccactcg cgtccaggga
     1321 cgcgtgggcg cccggtcgca ggcaatgcgc acccggctgg aactcaccga ggatgccgtc
     1381 gtcgcaaccc aagccctgct gagcgaagtg aaggaccttg attacaccga ggcggttacg
     1441 cgctttcagc aggctcagac gatcctgcag gccaacctga tgaccggtgc gaggctgatg
     1501 cagttgtcgc tcatggatta tctctgagcc ggcgatggcg gcgccggatg ggggagcgga
     1561 tgaggccggc ggggaacgag agcaaggacg gggcggagca cgtcgggctg ccggaccgac
     1621 ccaccagcag cccgaagccg tgattgcccg gcagcggtaa gccgggtcgc gttcaggatg
     1681 acgcaggtcg tccttctgca actggaggtg gtcgctgaga ccggtggtac ggacgttacg
     1741 gatgacgccg gccggtcgac gacatgcaga aggaccgcca atcacctgga atgggtcgag
     1801 atgaggagag tcgcgatgat gatccaaacg tcgcgctttg gaccgctgga ggtggatgag
     1861 acaaggctga tccgtttcga gaaaggaatc ctgggcttcc cggatcagca tgagtacgcc
     1921 ctgattcaga cggccgagga cagcggattc tactggcttc aggccgtcga ccgggcggag
     1981 ctggcgttcg tcgtctgtga tccgcgtctg ttcgttccgg attacgtggt tccggtgaaa
     2041 ctggaggagc tcacccagat cgggttgacc gatccgtctg gggcacaggt gtttaccatc
     2101 gtcaacaagg tggacagcat cctcactgga aacctccagg gaccgctggt ggtcaacgtc
     2161 gagacgcggc aggccaagca gttggtcttg tccgaccgca gatactcgac ccgccatccg
     2221 ctgatgaacc tcagccggaa gcccgaggcc gtgagcaaga cggcctgacc cgcatcgccc
     2281 ggccgcaaca acggggtgcc tgttcagccg gaaccccgcg cggtacgata ctgcttcggc
     2341 ctgggcggat gcgttgacat cggctgcttg ccccgcgagg aaagtgatgg tccgctcgcc
     2401 gagcgaacac accagccgtt tgcaaaccgg cacacggcgc ctcgatgatc ggacaagcag
     2461 gttattcacg aagcatcggc atggccaagg aaggagccga ggatgttggt gctatccaga
     2521 cagcgagatc agacaatcat gatcggcgac gatatcgaga tcacggttgt cgatatccgc
     2581 ggagacaaag taaggctcgg gatcaacgcc cccgccgaga tcccggtgca ccgcaaggaa
     2641 gtctacgagg cgatcaagag ggaaaatcgc gcggctgccg gcgtcaagcc ggaagacatc
     2701 gccaatgtgg ccggcacccc tgcgaatcat tcgggatctt cgaaggagag gtagggggca
     2761 ccgagcccgc ggcccggggc tttcgagcaa gctcctcgcg ggacgatccg tcaggtagaa
     2821 cggatctggt agccaagatc aatcaaggag gattgcaaaa tggctaggat taacacgaac
     2881 gtaccggcca ttgtcgccca aagagctctg cgccagtcac agagagaact gcaggtttcc
     2941 ctggaacggc tttccagcgg tctgcgaatc aatcgaggcg cggacgaccc ggccggtctc
     3001 atcagttcag agctcctgag ggccgagatc tcaggtatcc aaaagtccat cagcaactcg
     3061 cagcgggcga tcaatatcat cgcgacggcg gaagcctcgc tcaacgaagt cgcctcgctg
     3121 ctgaccgaca ttcaaggcct catcgtcgag atcgccaatg acgccgctat gtcggaggac
     3181 gaaaaacagg ccaaccagtt gcagatcaac tccgccatcg agtccatcac gcgcatcgcc
     3241 aacaccgcgt cgttcgcggg ccgcaaactg ctcaacggcg aactggacta cgtcaccagc
     3301 ggcgtcgatc cgaccatgat cggaacgtac gcgattcatc gctgccagtt cggcacggcc
     3361 agctacatcc cggtcgacgt cgcggtcact gtgtccgcac agcacgcaca gcttcagatt
     3421 cgaaacagcg caattaccaa caccatcaac gtcgagatcc gcggctcgat cggaaccacc
     3481 gtgctcagct tcacctccgg cacgtccgtc accgacatca tggcggccgt caatcgcgag
     3541 agccaggcca gcggtattga agccgtgtac ttgaacgacg ccaatcccgc cagcggcatg
     3601 gcgttccaga gcatcgggtt cggctccgac gagttcgtcg aggtgacgct gctgcccggc
     3661 agcggcgctc tgaccctcta tcgccccgac ggcaccacgc agtcgtccag cacgatgcgg
     3721 tccatcggac gcgacgccat cgccagcatc aacggcgccc aggtcatcgg ccggggcttg
     3781 gagctgaacc tccagacccg caccctcgaa ctcagtctga cgctcgacaa gaacttcggc
     3841 atcggatcca cgaccttcgc cgtaaccgga ggaggcgcac tcttccagct cggtcccgac
     3901 gtcaacacca accagcaggt gaacctgggc atccagtcgg tcgcggcgtc gcggctcggc
     3961 aatccgctga tcggttacct cagccaggtg atgtcgagcc aggcgttcga tccgacgagg
     4021 agcctgtcca acgccaacta cgcgtcgcag atcgtcaacg aagccaaccg gcaggtgtcg
     4081 gtgctgcgcg gacgactggg cgcctttgaa cgaaatacgt tgcagaccaa tatccgctcg
     4141 ctcgaggtga cgatggaaaa cctcatatcc agcgagtcgg cgattcgcga cgctgatttt
     4201 gcctatgaga cctcgcggtt gacgcgtaat cagattctgg tcagcgcggg caccagcgtg
     4261 ctggctctgg cgaattccag ctcgcagaac gtccttgcgc tgctcggcgg ataagactgc
     4321 gcctcaacct ggcgcgggtc gcgcacggcc gccttcacgc tcagcggcac aggctcagct
     4381 tgatcgcttc tgagcgcgtc ggcctctcct gagcacctgc gcctcagaag caaatcgtaa
     4441 tggccgaaac gatgcgcatc cgccgcaaat cgagctgacg aatgcggatc gcgggcgccg
     4501 ggtggcaggg tctcgaccgg ggcccgcgag cccgccccaa ggccgatccg cgcgccgccc
     4561 cgaggcaagc ccgcgttttc tgcacgtttt cttcgtccgg atcgcgacga tcacgccgtc
     4621 tcgtattctc ggtccttgac gccgttgtcc ggtaaaagga ccgggcaaaa gctcccaaac
     4681 gacctgctgc ggtttatgag acctgacaac gacggactgc ctcgcaagcc gaagtcccga
     4741 tccttctctg gcgtcgggat aaaactaaag cgggcgccgg aagctgacga tccgaccttc
     4801 gccgtcgaag gatcggcgga cctggcttcc ggcttcccat gcaaagtcga cgcagcagct
     4861 gagcgttgac cccgggacgg atgaggttgg acagggaagt tgaatgctga ccgcaggcac
     4921 ctgtatcgac gagtcgtcga taggtcccgg gtgatgcggc agagaaagag cggacactgt
     4981 cctgcgggcg aaccgccaga tgggcgccgc accgcaaacc gccgatcgga ccggaactcc
     5041 acagggggac acgtccgggg agcgccggaa gagccggtcg cggctggttg gaggcacgta
     5101 caatgagtcg tattatgacc aatgtgtcgt ctctggtggc ggcacgaatt ctggacagca
     5161 acaacaagat gctgaatacc tctctggaga ggctcagcac gggctaccgc atcaaccgcg
     5221 gaaaggacga tcctgcgggc ttgattgcat ccgagaccct gcgaggcgaa atcgcggcca
     5281 ttaaagccgc catggacaac accgagcgcg cggacatggt tatctccgtg gccgaaggtg
     5341 gtctgcagga gatcagcaac ctgctcgtcg agctcgaaag cctcgtcgac accaccgcca
     5401 acgaggcggc cctcggatcg gccgaggtcg aggccaatca gctccagatc gactcgattc
     5461 tcgacaccat caacagaatc gcaacctcga ccgagttcca gggcaagaaa ctgctcaacg
     5521 gaaccctgga ctacacaacg tcgggcgtca ccatcgggcc gtccggcggc tcggcgatcg
     5581 cagccctgca gatcaatagc gcccggatcc cgccgggcgg ctacaggacg gtaaccgtcc
     5641 aggtcacaac ctccgcgcag accgcccgcg tctatgccag cggctcagcc gttgcggccg
     5701 gtggtgttac cctgcaggtc agcggcaagt acggcgtcga gcagttcagc ttcggctccg
     5761 gtacgagctt ctctgaggtc gccaacgcca tcaaccagtc gaaggccctc accggcgtct
     5821 cggccagctt cagcgctacc ggcggcggaa ccctgatgtt caacagcacg gattacggtt
     5881 ccgaggcgtt cgtaaccgtc cagaccatcc agggcaccac cctcgcggtc agcggcggcg
     5941 acagcaccta cggcgaccac ggtgtcgacg tcggattgac catcaacggc gcccaggccg
     6001 ttaccaaggg tctcgacgcg tcgctgcaca ccaccgtgct cgaccttgac cttactctcg
     6061 acgcgggctt tgctcagcaa acggttctca gcaagacgtt ctacatcaac ggtggtggtg
     6121 ctgatttcag catcgctccg accctcgggt tgaacgccac cgccagtctg ggcatccaga
     6181 acgtggcaac gggcaatctg ggcaagggag gccttggtta cctgtccagc cttggcagag
     6241 gtcaggacaa cgacatggca tcgggcaact tcgcggtcgc tcagcgaatc atccgacagg
     6301 ccaacgagca gatctccagc ctgagaggcc gtctgggtgc gttctcgaag aacaccctga
     6361 cgtcggcata caacgccctg gccgtcgctc tggaaaacac ctcggcggcc gagagcgcca
     6421 ttcgcgatac ggacttcgcg agagaaacca gcgaattgac gagagcccag attctggtca
     6481 atgcggcaac ctccacgttg cagctcgcaa acgcgcagcc gcgaaacgtg ctggctctgc
     6541 tcggcggttg atcagaccat ggaccggtga tatgagccgg ctccgacggg caagcctgtc
     6601 cgctaactga actcaaacgg gaccaaatag cggtcgaacg cgacgagagt cgcgctcggc
     6661 cgctttctat tggccgagcc gggactgccg ccgaaaaata tcggaccttc aaacaaccga
     6721 ccgcttgtcc ctctgacgtc cgatatccgc ttgcaggctt ccggttctga aataatcctc
     6781 cgttaacgcg ggccaataac taaaggatgc acccgttccc tgtcgaaatg ccgattgtcc
     6841 ccatcggacc cgttcttcag gacggaatcc aaggaggtgc gaaggaggag tcgtcggttg
     6901 ccggccaacg ggtcggcagc agcccgccgc ggatggcggg aggtcgatcg ggaggatcga
     6961 tctggacgat gcggctcttg ccttcgcggc cgcagggccg ccacggcagg cctgccccaa
     7021 agggaggaat cccttgcgga caggtggagt catcgctggg acaccacgtc ccggcctcgg
     7081 agcgcaaggt cgcgctcccg gcgtagcagg ctttcctggc ccgctcgcgc gtcacggagg
     7141 atgccatgcg tatcaacacc aacgtcgccg ccatcgcgac aaccaaccgc ctgatcaaca
     7201 accaggacga cctcaacatc cgcttggagc ggctcagcac cgggttgagg atcaatcgcg
     7261 gagcggatga tcctgccggg ctgatcgctt ccgaggtcct tcgcagccag atcaggggaa
     7321 tcagccaggc cgtcgagaac tcccagcggg ctatgaatgt catcagcgtt gctgaggcgg
     7381 cccttaacga ggtatcgtca ctcattctcg acctgcgcag cctcatcgtt cacgccgcga
     7441 atgaaggggc gatcagtcag tctgaaatcg aggccgatca gctccagatc gactcaattc
     7501 tcgaagctat cgatcgaatc gcgaatacca cgacttttgc cggccgcaag ctcatcgacg
     7561 gcagtctctc ctacaccgtc tcgggtctgc atacctccgc aatcgcccgg gcgatgctct
     7621 tcggggccag agtgcctgat tacggcgcta cccatgtcac tgtctccgtg atccagtcgg
     7681 cgcagaccgc gcagttggtc tatgccggca gctcgatcgg ttacaacgtg acactcgagc
     7741 tccagggaac ccttggcacc gaaatcgtgg ccattaccag cggctccagc gtggagcaaa
     7801 tcgccgcgaa tatcaacgcc agcaccgttg tcaccggagt ctccgcaacc gtcagcggga
     7861 cgggcgctaa cgcgcggctt ctgctcaata gcgtcgagtt tggcgaaaga gcctttgtgt
     7921 cggtccagcc cctggagggc aatttcatcg tggccgaagg tgccaggatt caggacaacg
     7981 gccaggacgc cggtgtgctc atcaacgggc aagtggctgc gttcgatgcc ttgaccggcc
     8041 tgctccgggc caatggcctt gacatggaaa tcgagctgac cgtggcgggt gcgacgacga
     8101 ttgccagcac ggatttctac atcaccggcg gcggagcaac cttccagatc ggaccggatg
     8161 tcaatcccgg aggccagatt tccattggta tcccgtccat cggtaccgct cacctgggcg
     8221 atgtccagaa tggcttcctc aacacgattc gcggggccgg tcggaactcg atcgtcggcg
     8281 gtcactgcgc cgaggccgaa agggtggtca ccaaggccct caatcagatt gccgtcttac
     8341 gcggtcgact cggatcgctc caacgcaatc agatcgaaac caacatgaac agccaacggg
     8401 tggcccttga gaacgttatg gcgtcggagt cggccattcg tgatgccgat atcgcaaccg
     8461 aggttgccaa tctgacccgt gcccagatcc tggtgcagag tacctccacg gtcttgggta
     8521 tcgccaacca gttgcctaga aacgtgctct ctttgctgca gggctagggc ccctgcggtg
     8581 ggtgagcatg ccccaattac gtactgtccg ataatcttca gtccggcagg tcgaaccggc
     8641 tggttctgat cctgagaagc cgtcgtatct ccaccgctta acggtgaaga tccttctatc
     8701 gcaacaccga tgaatacgta gaccagtagt agtctgcgcg caaacgacgg aggtcgcctg
     8761 atgggtacga tttcaacagg ggtggggctg gtttccggac tgaatatcca ggatattgtc
     8821 agccagctca tcgagatcga gagccgcccg atcaaacgcc tcgagcagcg cgtcacggac
     8881 acccaggcac ggcagaccgc ctacaccgag ctcagcgcct tgctgctcgg agccaagggc
     8941 ctgatccaca cttttgtgtt gccttcggcc ttcaaggtca agagcgccac cagctccaac
     9001 gaaagcgtct tgacggccac cgccaagaca acggcaagca cgggcagata cagcttcctc
     9061 gtcaagtcgc tggcaacctc ccatcaggtc gtgtcctccg ggtttgctga tcccgaccgg
     9121 acccctgtcg gagccgggac cctcattttt gccccctccc aggcgcgagt ggatcccaag
     9181 acggccgttt ccacgctgaa cggtttcaac gggatccggc gggggatggt ccggattacg
     9241 gatcgaagcg gggcttcggc cgacgttgac ctccgagcgc cgaccagtgt cgccgacatc
     9301 ctcaatgcga tcaattccca gaccggaatc agcgtcaagg ccgaggtgga tggaggccga
     9361 atcgtcatca ccgacacgac gggcctgtct tcgggaaatc tcgtcatctc cgatctgtca
     9421 ggcggcggcg cggcaagcga tatgggtatc gccggcacct tctcctccgg ccgggccgtc
     9481 gggaaggaca tgttgtggat gaccgccacg acgcgcctga cggccctcaa cgatggtctg
     9541 ggagtccggt caaacggcct gctttcggac ctgcggttcg agctgaaggg cggcgaggtg
     9601 tttgaggcca ctctatccga aaacctccgc ttcaccatga acctggcaat gctcaattcc
     9661 ggcctcggcg tgcgcgccga cgaaaccggt cagcgagtca tccgcatcac gaaccgggcg
     9721 ggcgccggcg ccgagattga tctttcgtcg gctcaaacct tgcaggatgt gagtaacgcg
     9781 atcacgaatg ccgggctgtc gctcggcgtt tccatatccg gcaacaaact ggttatcacc
     9841 gacaggtccg gaggtaccga ccgcaacctg cggatcgagg atgtcaaagg ttacgccgcc
     9901 gctgatctcg gcatcaccgc ggacaccgcc gagagtgggg taaccggtcg cggaatctat
     9961 cggatcgaga cgattggcga cgtgctccgt gccattcagt acgccgaggg caacaatggc
    10021 tacgtcaccg ctgccctatc ggcagacggc aagggaatca ccatcaccga caacaccacg
    10081 ggtcttaacg acacgctggt ttcagcggtc aacggatcgc ttgcggctta cgatctgggg
    10141 ttgctcgact cgcgatccgc gaccgagcgg tctttcagcg gcagcacctt caccggtagc
    10201 gatctcctgg gcggcctgaa caccgtcatg ctcaagtcgc tcaagggagg tgcgggcgtg
    10261 cgggtcggcc tggtcgaatt cacgagggca agcggagaag tgttttccct ggacttcaca
    10321 agtgccaaaa ccctcgcgga cgtgatcaac atcatcaacg ccgatggccg tttgcacgcc
    10381 gaggtgggcg tgaatgggac cgagatcgcg atcaccgaca cgagcggtgc cactgggccg
    10441 atgtctgccg ccggttctct tgccgaagat ctgggcctga cggatcgggg cggagtgctc
    10501 gtcagcggca acctgaatcg ccagttcatt tccgaaaaca ctctgctggc ctcactacgc
    10561 aacggtaagg ggattcgtta cggccagatc cggttgagcg acagcagagg caattccgcc
    10621 acgatcaccc tgaacgagga tgtccaccgg acggtgggcg acgtgctgcg ggaaatcaat
    10681 cggttgttcg tcggcgtcga ggccggcatc aatgaggatg gtgacggtat caaactggtg
    10741 gataccgccg ggggcacgct gaccatgagt gtaagcgacg tggcaggggg gggcgccgcc
    10801 gccgatctgg gtatcaaggg atcagcccag aacggcgaaa tcacgtccag ctaccgtagc
    10861 agaatcgagg tggacgccga tgacacgctg tacgacgtgg tcagcaagat caacgatgcc
    10921 aaaatcggcg ttcaggcgaa cgtcatcaac gacggatccg cctggcagcc ttatcgactg
    10981 acgctcacct cccaaaccag cgggctggca gggcaaatgg ccctccatac cacgcccggc
    11041 atgatgaagc tgaatctgct cagcgacgcc agggatgcgg ttgtcgtcgt cggcgatctg
    11101 aactcctcca gcgcagtggt gatggcaagc ggcagcaact cgatcagcaa tgccattcgc
    11161 ggtgtcacgt tgaatctgac ccagaccagc gacctccccg tgacggtcaa cgtccaaaac
    11221 gatgtcgata cggttgttgc gggcatcaac ggcttcgtcg aggctttcaa cctcttcgtg
    11281 gataaggtcg cggagctgac caagttcgaa gccgccacca atacgagagg cttgctgctc
    11341 ggtgatggca caaccatgca ggttcgcgat tcgctctacc gcgtcctctt ggccaatgtc
    11401 aacgattcat cgctcaagta cacgaacgtg acccaggtgg gcttgaagct gatgaccaag
    11461 agcggcggaa aactccagtt tgacgaagag aagttccgac aggccttcga ggaagacccg
    11521 gacgccgtaa atgagctttt cactcgcatc gtcaagaaca aggaaggaaa gtctgttcag
    11581 atcggcatcg ccggccaact cgatagcctg ctgtcgcggc tgaccagtag cgtcgacggg
    11641 acattgaccc tccgtaacaa gagcctccag gatgaaatcg atcaatatgt caagcggcag
    11701 gaggagcttc aggagcggat agatgccaag gaagctcaac tgtacgccaa gttctatgcc
    11761 atggagacgg ccttgtcttc gcttcaaagc cagcagagcg cattggccgg ccttgccgcg
    11821 ttggtcaact cgacaaagac aaaatgatgc aggaggcgaa aggacacgcc ccgaacgccc
    11881 gccggcgcat attctcggac catgttctct gactccatcg ctattgtcct tgccgtcatg
    11941 gcgaatgagt tggcgccggg agcttcccgg aaagtggtga accgcgcccc gcgcgtaacc
    12001 gataacatca ccaacgatga gccgggaacc gaatccatac ctccgagacg ccatcctgac
    12061 cgccagcccc gagcagctcc agctgatgct ctatgacggg gccatccggt tcacccgaca
    12121 gggcatcaag gccatcgagg acaagaactg ggaacagtgc ttcaatgcat tcaaccgggc
    12181 tcagcagatc atcctggaaa tgctgaacgc tctcaattac gacgttgacc gcgagctgtg
    12241 caagcggatg gccggcatct ataacttcat ctatcgcaag ctcatagagg cctgctctag
    12301 ccgcgagctg caagctgcca acgacgccct gggattactg gagttccagc gggaaacctg
    12361 ggtgatgctg atagaaaggc tcaagcagga acgctccggc gtgaactcgt cacaagccgg
    12421 cgacgcccag tcctccaagg tcggcgaaca gacggccgct cccagttatg ggacgctgtg
    12481 tctcgaaggg tgatcctcgg cctgccttga ccgtcttgaa ccccatccat atagtcccaa
    12541 acgacttttc ttgcataggc ggccgtcgcc tgcccgatgt cttcttgtga cgcccggaat
    12601 gggacggctg ttcaggtgct gggacagacg atcctggcct tggccgtccc ggaaccttac
    12661 ggccagggat gtgggccgct tgcgctcatg tttccggatc gtaccgcgct atctttgcgg
    12721 cccgcaaaaa ggcggtccgc cttgggcagg aggcttcatg atggcgaaag aaaaccctgg
    12781 gccccttggc gatgggcttc tgacaggctg cttctcggaa aaggcagtga caaaggctgc
    12841 gcatgaccga cggaaatcgc ctccccggct cacgcacttc gtcgaacgcg tgaaggcggt
    12901 ttcaggttcg ctgttctttc tgattgcctg gaccatgctc cagttgacac cggcccccgc
    12961 gcagaatgtc gcgacaacga cgcaagccga accgccttcc ggggcattgg ccggtttggc
    13021 tggaatatcg gaagtgaagc cggggatgtc aaccaaggag ctgatcgagc tggctcgcca
    13081 attggcccaa tcccacagat gggacgatgc ggaaaagata ctcaccgagg tgatcaggca
    13141 ggaaccccgc aatctctcgg cataccagtc ggcggctcag atgtacgaga ttcgggcgtc
    13201 ggtcgttcgg gcggatgctt ctctcccgga tgcggccgag aagagcaaca cgcttatcga
    13261 ccaggccgtc aagacctaca cggaatacgc cggcccgctg gcattggaga tgggggacgt
    13321 tgccacggca gaggagatct acaggaccgt actgcgatac gagcggcaca ggtacaatcc
    13381 gcgggcgctg ctcggaatcg cacgtgtcct ccgggcaaag ggtagccccg aggccatcga
    13441 tcgctacaag acatacgtca atcctcgcat ctgcatggac ggagctaagg acccgcaggc
    13501 tcacctggaa ttgggtaggc tcctttatga acgcagatac ttgaatcagg ccgtcagcac
    13561 gcttgaaacg gcgagatcgc tcgatcctga aaaccctgag attctcctgg aactcgcccg
    13621 agcttaccag gaaaccgcgt ttcgccccaa ggccttgtcg gtggctcagg aagcggtgaa
    13681 gaaaagcccc ggcaaccccg cctatcgaag cgtccacgcg cagatactcc ttgcccagag
    13741 cggggtgcaa caaaagggaa acgccatgtc cgacgaggag cgccggatgt tcaatgccgc
    13801 ccgggctgaa atcgaagaag cggtccggct ggcccgtacc ggcctccagg atgcaccggc
    13861 ggacatgact cgcctcgggg ttctctccgg gtgtttgcag gttcagcagc agatcctgtc
    13921 gattgcggcc gccatcaacc ccggggatgt ccgtctggtg gtggacttgg ctcggtgtcg
    13981 ccaggaacag tcggcaaccc aacatgctct tcagcttcac ggcgccctca acgacttgct
    14041 gcgtgcgcca ccggccgccc gcggagatgc gtcctacctc gagacgcttg ccgatctgca
    14101 gttccgcgtg catctggtca cggatgctga attgacctgc aaggaactgc tgacgttgga
    14161 tccgaacaac gctgttgcca agcagattct cgaaaaggtc gccgggagca gcggtatatc
    14221 ccccccggga ccgggcgccg ctccttcgaa ataggccatg agacctgcct tttcaacctg
    14281 cggtttcttg tgtaaaatca ccattttggc ccggggtgcc ggagacgccg atgggtgatc
    14341 ggcatatttg ctcccggcgg ttggttccag cctctacaag cgaaaccgct gattgtgatt
    14401 ccgggcgacc ggcgcggccc tggggagacg gggcatgccg gcgggggggc gaacgtcgcc
    14461 gggctgtcgc cccgagcaag gaaggatgag agatatgtcc gatttcaaga tccagccgtc
    14521 gcagcggttg cttcggctgc cgccctacct cttcgcgaaa atcaacgccc tcaaacacaa
    14581 gaaacgtcag gagggcatcg atattattga cctggggatg ggcaacccca ccgaccccac
    14641 cccccagccc attgttgaca agctttgcga ggcggcccgc gatccccgca accagcgata
    14701 cagtgtgtcc aagggtgtgc tcaacctccg ccgggatgtc gcccgccact accacgaacg
    14761 gtacggagtc caggtggatc ccgagaccga ggtcgtggcc tgcatcggca gcaaagaggg
    14821 tatcagtcat ttgctcctcg ccatgcttgg acccggtgac accgctgtcg tcggagatcc
    14881 cgctttcccg atccacgtct acggcgtggc cttggccggc gccaacgtga tctcggtccc
    14941 gttgggtaat gatgaagcct tcctcgaacg gattcgttac gtggtccagc atctttatcc
    15001 gcggcccaag ctcctgatcc tcaactatcc gcacaacccc accgccatga cggtcgaccc
    15061 gccgtttttc gacaaggcgg tggcgctcgc cagggaattc ggcgtcgccg ttctccacga
    15121 ctttgcctac ggcgagacct gcttcgacgg atacaaagcc ccgagcttcc ttgcggcaga
    15181 gggagccaag gaagtaggcg tcgaattcac taccatgagc aaaccctaca atatggccgg
    15241 ttggcgcatc ggcttttgcg tcggaaacca gaccatgtgc gaggcgctcg gaaccatcaa
    15301 gggttactac gattacggca tctttcaggc cgttcaaatc gcttcgatca ttgccctgcg
    15361 gcattgccgc gagtttgtcg aagaacaggc tcaggcatac cagcggcggc gcgacgttgt
    15421 ccttgagtgg tgcgagcgcc tcggttggca ggcggagaaa tgccgggcga gcatgttcgt
    15481 ctgggcacgt gtcaaggatg aacacttggc cggccggtca tcgattgatt tcgccatgcg
    15541 gatgatggag gaggccgagg tggctctggc tccgggccgg gccttcggtg agaacggcga
    15601 gggctacgtt cgaatcgcct tggtggaaaa cgagcagcgc ctgcggcagg caatgcggca
    15661 gatccaccgt gcaatcggca aaaagccgat caagggtaca ccgctccgaa aaaagcggcc
    15721 tgccaatccc ggtcggcaaa cgcaagcccg gcagccctct gaagtgaaca ttcccgacga
    15781 ataggagccg cacgggtccg cggtgtcgaa tcgtgatagc catcgttgac tacgggatgg
    15841 gcaacctgcg cagcgtccag aaggctctcg aacgcgtggg agcctctgcc gccatcgtgt
    15901 ccgccccttc cgacgtgcag cgggcggaca agatcatcgt ccccggcgtt ggggctttcg
    15961 gtgatgcgat agccaatctt cgtcagaggg gcctcgtcga accgctcgtc aaggcagccc
    16021 gatcggaccg accgttcctg ggcatttgtc tcgggctcca gctccttttt gacgtcagct
    16081 acgaggaggg cgaacaccaa ggcctcggca tcatcccggg gcgcgtgttg cgtttcgatt
    16141 tttcggggcg gcccgaggcc gccggcctca aggtcccgca catgggatgg aatcaggtgg
    16201 cctggaccgg gtcctgtccg ttgttggagg gcctcacaag cccccagtat ttttacttcg
    16261 tccactcata ctacgtgtcg ccggttgatc gctccgtggc ctgcggcctg tgcgactatg
    16321 gcggtccgtt cgcggcgatg atttgccgcg gtaacctcta cgccacccag tttcatcccg
    16381 aaaagagcca gaaggtaggc ctgagaattc ttgagaactt cgctcgcttg tgaagggact
    16441 tgagcgtatt tcccccgggt tgcacaagaa tccccgcagg cgctcgcccg cgtcggcacc
    16501 acagtagggc cgacgccccc gtcggcccat ctccgctcgg cccggaatcc tttccgggcc
    16561 gcatcccccg cggaatcctt tccgca
//
LOCUS       DAOTPW010000124        16553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3478_length_16553_cov_5.574373, whole genome shotgun sequence.
ACCESSION   DAOTPW010000124 DAOTPW010000000
VERSION     DAOTPW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16553
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_16553_cov_5.574373"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="P5316_12300"
     CDS             complement(<1..363)
                     /locus_tag="P5316_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85780.1"
                     /translation="MREQKQHIQLLCVSVAVIMSCFVAVSAIGEVTFDGVIGFGAGEE
                     WANAESTAVQDNYTGYGDQVWNDPAVVGSELDELYVRTDGTYLYVGVTGNLQFDGNAI
                     VLLLDTDLTPGVGQNVLAT"
     gene            complement(921..3251)
                     /locus_tag="P5316_12305"
     CDS             complement(921..3251)
                     /locus_tag="P5316_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85781.1"
                     /translation="MRNDCWVAAMSVLWPALWCAGASTSTSAPVAKLAPAARAIIGCR
                     GTYDAAPRLPNGRVDLDRLLLELGDLRANTYHWLIWHADTDWEDLQAFLPRAGEAGLK
                     VWVTLVPPSESPPHTKRFSEPYRLDYERWAVEIARLSRKHPHLVAWSIDDFTHNLGVF
                     TPARIKAIRASTREIHPQLAFVPCSYYSAVTPAFARDYAGLVDGLLFPYRHESGGANL
                     TDPGLVEAEVARLRELMGVSVPIIVDVYATAHSRLGDSTPEYVEQVITAATACADGVL
                     IYCHQDPVKQAAKYQVIRQQFRASPAGDVPSMKTSLLVGAACVDITPAGPVALQGQFN
                     LRIARTIETPLTANAVTLEARAGEKSLDAAVMVSCDLISIPDVVLHSVREEIRKRLPE
                     LGADKIILNGTHTHTAPVMEAGRYELPKEGVMPVEAYRAFLVERLAEVIAKAWTSRGP
                     GSVTWGLGHAMVAYNRRAVYVDGSAVMYGRTDVPTFRGLEGYEDHDVGTLFFWNKDGR
                     LICIGVNVSCPSQVVESRATINADFWHPVREALRRQYGPEVCILGWTGAAGDQSPRPI
                     HRKAAEERMMRLRGLEPTEELARRITRAVDEAYEAVKNDRHADGPLIHRVETVQLPMR
                     LVTEAEYAEAKVACRQVADEIAKDPKAADRLHRRMKWYEATVQRFESQKDNPNPTHET
                     ELHVLRIGDAVICTNPFELFTDYGVQIKARSKAVQTFVIQLAGGGSYLPTERAVRGGG
                     YSAVVHSNKVGPEGGQMLVDRTVEAISALFGPHPSQ"
     gene            4160..4909
                     /gene="nagB"
                     /locus_tag="P5316_12310"
     CDS             4160..4909
                     /gene="nagB"
                     /locus_tag="P5316_12310"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRR85782.1"
                     /translation="MEVVICPTHDDLSIRAARIVAELLRAKPDAVLGLPIGSTPIGLY
                     RELVRMHREEGLSFARARAFTPIEYVGLGPEHPQSSHRFMRQYLFDHVDMCPDKVYAP
                     SGLALDLDAHCLFYEQQIADCGGIDLQILGIGTDGHIAFNEPGSSFDSRTRRVALARQ
                     TIDDNARFFPSRQHVPLMAVSLGIATILEARRILLLASGPNKAAILAAAVEGIVNEMC
                     PASALQLHEHVTVLADESAASRLTLSAGQGR"
     gene            complement(4958..6334)
                     /locus_tag="P5316_12315"
     CDS             complement(4958..6334)
                     /locus_tag="P5316_12315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRR85783.1"
                     /translation="MTDLATLIILAAGCAAWWATCPCDVSSRRKAALAAIPPAGMLIV
                     RAVGGAGLALDLASVGMVVLVGLLAGYWLVVRREWPKGAMVLALAAVTAAGTFWRQEV
                     MEQVRANPLQPDVRHFQQQAQRTFNPYAAGLKSPLWSAMHAPLLRLAANPDHAMRFWS
                     WVFGVAMLPIVGLVIGRLFDPVVGVVVAGLLAADSYLANLCCEGLREEMGLCLWMAVL
                     LLLFEGDSRSWKRVVLAGAAGGVLIWLRNVELVPLAALPAWAAISRRWTLLQAAVALL
                     LSLLIVLPFYVNQYRTYGNALAMEQREARGLVNLEFLGQSVPADVAMPTAEQVAADPF
                     AGEPVSPAKYLFGMRSPRDLVSRQLRGLYHVLAGRSFEYGYSRWTALACGAGLVATVL
                     SRRHRFVIVMVLFSLLGLRAHMVSLGHVDLRLLLPVMVMWLTAGTWFAALLVRLGGRR
                     WLTMKAEG"
     gene            6639..8843
                     /locus_tag="P5316_12320"
     CDS             6639..8843
                     /locus_tag="P5316_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HRR85784.1"
                     /translation="MSDLRILPIRTLGHGFIEEPYLPPGKDEYYLRDQQCPSRPDRYR
                     SLRADEIEVLIRNGNTADSWDNIRVTEQFAPHRIKRCEFYGLVRIGRLEEMMLEHHDM
                     RVPVGITDSRIIACDFGDDVAVHHVRYLAHYIIGDHCILLNIDEAHTTNHAKFGNGIV
                     KEGESEEIRVWMDLINEAGGRRVMPFDGMTTGDAYLWSRYRDRPALMTRFAEITQRMF
                     DTHRGIYGTIGKDCVIKNTGIIKDVKIGDCCYIKGANKLKNLTINSTEAEPTQIGEGV
                     EMVNGIVMPGCHVFYGCKAVRFIMGYNSNLKYGARLIHSYLGDNSTVSCCEILNNLVF
                     PAHEQHHNNSFLIASLVMGQSNIAAGATIGSNHNSRAPDGEVHAERGFWPGLCVSVKH
                     NCRFASFTLLARGDYPAEMDIRLPFCLVSNDPGGDRLRITPAWWWRHNMYALARNTWK
                     FRARDKRKCKCQHIEFDYLAPDTAEEIFTALRLLEMWVAKAALPSRGESAEHMSPDAL
                     AETGRRLLVESPDEVDRLEVLAEGAEKSSRKVVILKPRQGYLAYRDMLHYYGVRNLVD
                     YLMADPQATLASAAEALAGPQWRNWVNVGGQIVRADDLETLLSGIEQGLHQTWHAIHR
                     VFDSWWAKYPLDKQQHAFACLTTLLGSNSPSKDQWDAALDRAVEIQHYIADAVFSSRK
                     KDDEDPFRRMVYRTPEEMTAVLGRAEDNSFVRQVREDTHAFISRIEEIRRRG"
     gene            complement(9021..10310)
                     /locus_tag="P5316_12325"
     CDS             complement(9021..10310)
                     /locus_tag="P5316_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85785.1"
                     /translation="MYGQTIIASALLATLSIAAGAAEPPAAKGWKQKQFIITFWCPPP
                     ATDEALANVAAENYNLTWVPVEGLDVAARHGLRAMLTSNLLSPDVLDDPAKRAQLDTL
                     IERVKRHPALEAYYITDEPGAGAFPGLGRLVAYLRERDPDHLAYINLFPTYATEAQLG
                     VSADAAERAKVGYPQNFAGVGTSDKTVLAYREHLKAFIEIVKPDLISYDHYHFLLTGD
                     GSQYFLNLALIRQAAQEANKPFLNIVQACNGEKVWRLPNAAEMRWLAYTTMAYGGRGI
                     SYFVYWMPKVYGGIYQDGKPSPLAKVVAELNGEMAKLGPWLLQLDSTGVYHTAPLPYG
                     TQAVPADSPVQIVGKGEFVLGLFGASGRTTSFMIVNRSYKEPAEAELKIAIPGRKLQE
                     LDRKTGKWSEGETLPADRQVKVRLDPGDGRLFRTVER"
     gene            complement(10415..12937)
                     /locus_tag="P5316_12330"
     CDS             complement(10415..12937)
                     /locus_tag="P5316_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85786.1"
                     /translation="MPGTMDLNGRWGLTWAEGSGLMNPDHYTAPTLRGRKMLDAAVPA
                     PIHTVLMNAGLIDDPNVGLNSLKARWVEEQFWIYRRSFVAPEESTKQHAWLVFDKLEY
                     EAVVWLNGQEIGHHANAHRPARFDVTGRLQEGDNLLIVKLSTGIHSAADKPGAEYHCH
                     LIDLLTKRPWHRKPQYQCGWDWNARLMNVGILGDVRLEWHAGPYLEQAAVFAIPSPDL
                     RSAKVHARAFVFNRGDSEVEGTLRVRIRETGQDISAKVRMVPGQSNHELIIDLADPRL
                     WWPIGHGEQSRYTVDVELTASGETQAVTRKTGVRRVEIDQSPHPEEGRHFIIRINNRP
                     IFCKGGNWVPPDLLYSRVTPQRYRDLVRLAVEANFNTLRVWGGGCFADHALCETCDEA
                     GILIWHDFLFACAKYPGDHPDFAAEVRREVTWAVRDLAHHPCLVVWCGNNEIEWGDWN
                     WGYDNKHRTHPHYALFHHDIPKIAVCENPATAYWISSPYSPDYMEPNDPTVGDQHPWG
                     VSILEPHGADFWKYRQYVDRFPNEGGVIGASSPATLRQFLSEDQRYLLSPEWDHHDNP
                     FACNAPTPGDLGRAYATVEMWTGLNPEEMQWEDYAYVSALMQAEGLQEYIANYRRRMF
                     SSSSAIFWMYNDSWPVTHGWTIVDYYLRKKLAYHPVRRAFQPVRVVVAEDKDQVLVFG
                     VNDSTSQWSGKLRSGLFRLAGGLEDDKRKTVTLPANASTVLATIDKNKWQQLGSASAG
                     AFAVLSKDGRMVSQHRLFIERFKDLKFVEPRIEMSVKDGILTLSSKAFAWGVCLDVDG
                     EAPLADNCFDLLPGIPYSLPWPSSLGEPKVVRLGNRDAVKRC"
     gene            13525..14712
                     /locus_tag="P5316_12335"
     CDS             13525..14712
                     /locus_tag="P5316_12335"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRR85787.1"
                     /translation="MIVGVVKESFPGEQRVALVPSSVTALMKSGFEVIIETGAGLAAG
                     YPDSQYHEKGTKVVPTRGEVFAGCDILLQVRTLGANPRAGRADLALLRRDQILIGLAE
                     PLMAAEAIRALAESGVTLLALELMPRITRAQSMDVLSSQGSIAGYKAVLMAADTLGKV
                     FPMMMTAAGTIQPARVLVIGAGVAGLQAIATARRLGAVVSGCDVRPAVKDQVESLGAR
                     FVALPVETAGTETKGGYAKEQTEEQLRKQRELMARVVAESDVVITTAAIPGKKAPVLL
                     TADMVRRMQPGSVVVDLAAERGGNCELTQPDRTVVDNGVIIIGPVNPASLVPCHASQT
                     FSNNMVQLLQHLIAKDKQVRIDTGDEITREMLVCRDGQILHPRVREAMGLTPPAGPER
                     SDG"
     gene            14712..14987
                     /locus_tag="P5316_12340"
     CDS             14712..14987
                     /locus_tag="P5316_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRR85788.1"
                     /translation="MLIMLLTIFVLAVFVGFEIITKVPPTLHTPLMSGSNAISGITIV
                     GAVLAVHAGHPILSGVIAFLAVTLAMINVVGGYLVTHRMLRMFKKKG"
     gene            14992..16428
                     /locus_tag="P5316_12345"
     CDS             14992..16428
                     /locus_tag="P5316_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRR85789.1"
                     /translation="MSDPTLNLVYLGASVLFILGIKGLTHPRTAVRGNFISAVAMLAA
                     VAVTIIFGHPHGLPIVLAGLVVGALIGVILAVKVRMTQMPQMVALLNGFGGAASLLVA
                     WSALIEKAATRAAATQPTLPPPEMAAVGDLQMTIATAASGLIGAVTFWGSLVAFAKLE
                     EIRRFNKPVFLPAHQLINFGLLLPAVLLGAWIVWQPEHGMWIYFLLVAVASALGVTLV
                     MPIGGADMPVVIALLNSYSGLAAAATGFVIDNNMLIIAGSLVGASGIILTRIMCKAMN
                     RSLVNVLFGRLGPTAATAGADDVYGGRIKSTTPEEVAMLLEGARRVVVVPGYGMAVAQ
                     AQYAVRDLYQELEKRGTTVEFGIHPVAGRMPGHMNVLLAEVNIPYDRLKEMDEINSGF
                     EQTDVVIVLGANDVVNPEARHNPNSPIAGMPILDVDKARTVVVIKRSLSPGFAGIPNP
                     LFAMDNCLMLFGDGKKEVVALIEALKRQ"
BASE COUNT         3151 a         5269 c         5052 g         3081 t
ORIGIN      
        1 cgtggcgagg acgttctgcc cgaccccggg ggtcaaatca gtgtccagca acaacacgat
       61 agcgttgccg tcgaattgca gattgccggt gacgccgacg taaaggtacg tgccgtccgt
      121 ccggacgtat aactcatcca actctgagcc gacgaccgct ggatcattcc agacctgatc
      181 gccatagccg gtgtagttgt cctgcaccgc cgtcgattcc gcgttggccc attcctcgcc
      241 ggcgccaaaa ccgatgaccc cgtcgaaggt cacttcgcca attgcggaca cggcaacaaa
      301 acaggacatg attacggcaa cagatacaca tagcagttgg atatgctgct tctgttctct
      361 cacggctttt ctccttttcc cgccgatggc ggcgttgcgc ctgagaccgt tccggaaccg
      421 tcgacctgac cggttggaat cgcagaatgc ccggagatgt gcaaaacgtc ctgtttccta
      481 ccctccaacg gcctgtccgg atattgtcct tgtactggga aacgaatacc gagtcaatag
      541 ggagtatacc gcatgctggg ctttgctggc acccgtttct tgcctgcgtc aagccctgcg
      601 ggagtccgta tcacccactc cggcgcgttt cgtcgttgcc tgcacgcgat gccgtcagga
      661 ccggactggg cggcatgcgg ttcatgccag aggcgcggca agagccgact aagtgattgt
      721 cggcaaacgg cttccaagcc ggttgaccgg caacctgcct gatctcggtg gtcggcaggc
      781 tgatgcacag ccgggaccgg caggtctccg ccttcttcgg tgactttgac catgcccgaa
      841 tgtttccacg cgcgttccga gagtcaaagg gtgtggtccg cctcgtctcc cgagcaggat
      901 ccagggttgt ctccatgtat tcactgggac ggatgtgggc cgaacagtgc gctaatcgcc
      961 tcgactgttc ggtcgaccag catctgcccg ccctcaggac cgaccttgtt gctgtgaacc
     1021 accgcactgt aaccgcctcc gcgcacggcc cgctcggtcg gaagataact tccgccgccg
     1081 gccaactgga tcacgaaggt ctgaaccgct ttgctgcggg ccttgatctg gacgccgtag
     1141 tcggtgaaca actcgaatgg attcgtgcag atcaccgcat cgccgatgcg cagcacgtgc
     1201 aattcagtct cgtgcgtggg gttcggatta tccttctgcg attcgaatct ctgaacggtt
     1261 gcctcgtacc atttcatccg ccggtgcaaa cggtcggcgg ctttcggatc cttggcgatt
     1321 tcgtcggcga cctgccggca agcaaccttt gcttcggcgt attctgcttc ggtcaccagc
     1381 cgcatgggaa gctgaaccgt ttccacccgg tggatcagcg gcccatcggc atgccgatcg
     1441 ttcttgaccg cctcgtaagc ctcatcaact gcccgcgtaa tgcgccgggc cagttcctcc
     1501 gtcggctcca gcccgcgaag ccgcatcatc cgctcctcgg ccgccttccg gtgaatcggt
     1561 ctgggcgact ggtcgccggc ggcccccgtc catccgagga tacacacctc cggaccgtat
     1621 tgcctgcgca acgcttcgcg caccgggtgc cagaaatcgg cgttgatcgt ggctcgactt
     1681 tcgaccactt gcgaaggaca ggatacgttg acgccgatgc agatgagtct gccgtccttg
     1741 ttccagaaga acagcgtgcc aacgtcgtgg tcctcgtagc cctcgaggcc gcggaacgtc
     1801 ggtacgtccg tcctgccgta cattaccgcc gaaccgtcga cgtagactgc gcgccggttg
     1861 taagccacca tggcatgccc gaggccccag gttacgctgc ccggcccgcg gctggtccat
     1921 gcctttgcga tcacctcggc aagccgctcg acaagaaagg ctcgatatgc ttccaccggc
     1981 ataacgcctt ccttcggaag ctcgtatcgg cccgcttcca tgacgggtgc cgtgtgagta
     2041 tgggttccgt tcagaatgat cttgtctgca ccgagttccg gaagccgttt ccgtatttcc
     2101 tcgcgtaccg aatgcagcac gacgtccggg atggaaatca ggtcgcagga gaccatgacc
     2161 gccgcgtcta gcgacttctc acccgcgcgc gcttccaggg tcacggcgtt ggcggtcagc
     2221 ggcgtttcaa tcgtccgggc gattcggagg ttgaactgtc cctgcaatgc caccggacca
     2281 gcaggcgtga tgtccacaca ggctgccccg accaggagcg aggttttcat cgagggcacg
     2341 tcgccggccg gcgaggcgcg gaactgctga cgaatgacct gatacttggc cgcctgcttg
     2401 accgggtcct gatggcagta gatgagaacg ccgtcagcgc aggccgtcgc ggccgtgatc
     2461 acctgctcga cgtactccgg ggtggaatct cccaaccggc tgtgcgccgt ggcgtagaca
     2521 tccacgatga tcggcacgct tacgcccatc aactcgcgca gccgggcaac ctccgcttcg
     2581 accaggccgg gatcggtcag attggcgccg ccggattcat gccggtaggg aaacagcagg
     2641 ccgtcaacca gccccgcata gtcgcgagca aaggcgggcg taacggcgct gtagtagctg
     2701 cagggcacga aagccagttg gggatgaatc tcgcgagtgg atgcgcggat cgccttgatc
     2761 cgcgcgggcg tgaacacgcc aagattgtgc gtgaaatcgt cgatgctcca ggcaaccaga
     2821 tgcggatgct tgcgactgag acgggcgatc tcgaccgccc agcgttcata gtccagccga
     2881 tagggttcag agaatcgctt ggtgtgcggc ggcgattccg agggcggaac cagcgtgacc
     2941 cagaccttga gcccggcctc gcccgcgcgg ggcaggaatg cctgcaagtc ctcccagtcc
     3001 gtgtcggcgt gccagatcaa ccagtgatag gtgttggccc gcaggtcgcc gagttccaag
     3061 agcaaccggt ccaggtccac tcgtccgttg ggcaggcgag gggccgcatc gtaggttccc
     3121 cggcaaccga tgatggctcg cgccgccggg gcaagtttgg cgacgggagc ggaggttgag
     3181 gtcgaggcgc ccgcacacca cagcgccggc cacaatacac tcatagcggc aacccaacag
     3241 tcgtttctca tgaatcaagc cccccggata accttgcttg gccggatctt ccagtcctat
     3301 tcggccattg tacgacggct catcccagcg cccaggtgac gagttgccgg ccctgaacgg
     3361 agggcccgtg acagaagcgg cggcactcct cgcggctgac ccggttgtcg tgaacgcaag
     3421 ccgaaagcag agcgtttcac cggtctttcc cgaagggcag ataggccgcc tttggcgggc
     3481 aaacccgctg aaaggccttg atcgcccaat ctggaccggt cggcatgccc gcccagactg
     3541 tcacgcacct ctgaacggat ctttgccgcc tgcggatgat ctccctcgac agaggaggcg
     3601 tcaggctttt tgccggggcc tgctgaggca ggcttgaaac actgtgaacg aggcatttcg
     3661 acgtccgttg accgactttt gcgatcctct cgttgacagc ggaacgtccg ccaacgtccc
     3721 agaactgcca ctcgggcggc aggcacccaa gttccctcct ggcaggcgct tgcggccgca
     3781 gacgaggtcg ccggcggtct gagcgggcgg acttatcggc cggctcggtc cagccggccc
     3841 atgtggcaaa caacggttgg aaaatgtccc gcgcgatgtt atatctaagg ggtagggaat
     3901 agtgtctgtc atgttcgaca gggagttatt cattgattga accgtaatcg gaacccagga
     3961 ggcctctgaa tctccaaagg gaacgagccc gctggacggg aggttctcaa aaccgaattc
     4021 aagccatccc ccttatagca gggttctttc aatcagctcc catcgacatt gcggcggatg
     4081 ctgttctctg tatagcagtg ccgcccgccg tccaccgacg gcgggcgtct ttttccccgc
     4141 gttgcgagga ccctgtgaca tggaagtcgt catctgtccg acccacgacg atctcagtat
     4201 ccgcgcagcc cggatcgtcg ccgaattgct tcgtgccaag cccgatgccg ttctcggcct
     4261 gccgatcggt tcaacaccga tcggtctcta ccgtgaactg gtccgaatgc atcgcgaaga
     4321 gggcctcagt ttcgcccggg cgcgggcctt taccccgatc gaatacgtcg gcctgggccc
     4381 cgagcatccc cagagcagcc atcgcttcat gcgccagtat ctgttcgatc acgtagacat
     4441 gtgccccgac aaggtctacg ccccttcggg tctggcgttg gacctggatg ctcactgtct
     4501 tttctacgag cagcagattg ccgactgcgg tgggattgat cttcaaattc tgggcatcgg
     4561 caccgacggc cacatcgcgt tcaatgagcc cggcagctca ttcgattccc gcacccggcg
     4621 ggtggccctg gcccgccaga cgatcgacga caacgcccgc ttcttcccga gccgccagca
     4681 cgtgccgctg atggcggttt cacttgggat cgccactatt cttgaggccc gccggatctt
     4741 gctgctggcc agcggcccga acaaggcggc gattctggcg gcggccgtcg agggcatcgt
     4801 caatgagatg tgtccggcca gcgccctgca gcttcacgaa cacgtcaccg tcctggccga
     4861 cgaatcggcg gcctcgcggt tgaccctttc ggccgggcaa ggacggtgag aaaaactgaa
     4921 ggattaaggc gggattgcat cgacagacgg tcttgactca tccttcagcc ttcatggtca
     4981 gccatcgccg tcccccgagg cggaccagca acgccgcgaa ccacgtcccg gccgtgagcc
     5041 acatcaccat caccggcagc agcaacctca ggtccacgtg ccccagggaa accatgtgtg
     5101 cccgaagccc cagcaggctg aacaggacca tcacgatgac aaatcgatga cggcgggaca
     5161 aaaccgtcgc caccagtccc gccccgcaag ccagggccgt ccagcgtgag taaccgtatt
     5221 caaacgaacg tcccgccaac acatgataca gaccccgcaa ctgccggctg acgagatcac
     5281 gtgggctccg catgccgaaa aggtacttgg caggtgagac cggttcgcct gcgaagggat
     5341 ccgctgcgac ctgctcggcc gtcggcatcg caacatcggc gggcaccgac tgccctagaa
     5401 actcgaggtt aaccagtccg cgtgcctccc gctgttccat cgccagcgcg ttcccgtaag
     5461 ttcgatactg gttgacgtag aacgggagca cgatcagcaa agacaggagc agagctaccg
     5521 cagcctgcag gagcgtccat cgtcgtgaga tggccgccca tgccggcaaa gccgccagcg
     5581 gaaccaattc cacgttgcgc aaccagatca gaaccccccc cgctgctccc gcaagcacca
     5641 cgcgtttcca cgagcggcta tccccctcga acagcagtaa cagcaccgcc atccataggc
     5701 acagacccat ctcctcgcgc agcccttcgc aacaaaggtt tgccaggtat gaatcggcag
     5761 ccaacaaacc ggccaccacc accccgacca ccggatcgaa caggcggccg atgaccagcc
     5821 ccacgatagg caacatggcc accccgaaga cccagctcca gaaacgcatg gcgtggtcgg
     5881 ggttggccgc gagccgcagg agtggagcgt gcatcgccga ccacaacggc gatttcagac
     5941 ctgcggcgta gggattgaaa gtccgttgtg cctgctgctg gaaatgccgc acatcgggct
     6001 gcaacggatt ggcccgaacc tgctccatga cctcctggcg ccagaaggtg ccggcggccg
     6061 tcacggcggc cagggccaga accatcgccc cctttggcca ttctcgacgc accaccaacc
     6121 agtaccccgc gagcaggccg accagcacca ccattcccac ggatgccaga tcaagggcta
     6181 gacccgcacc cccaaccgcc cggacaatca gcatgcccgc cggcgggatg gccgcaagag
     6241 cggctttccg acgcgacgag acgtcacatg gacaggttgc ccaccatgcc gcgcagcccg
     6301 cagccagaat gatgagtgtt gccagatccg tcacttcgat gctcctggtg acgcgaatcc
     6361 caaggccgac ggccttcttg cgggttgcga ttcaaggatt ctgcggtccg gaaacatgtc
     6421 cgcaaaacac gcgccggcgg cagtggaaaa gtctagtgac ccccgatggc cgaagcaaac
     6481 cccgccgtcc ggcggcgccc gctccaaaac aacacaagac caagacaggt gtctgccgac
     6541 atgttatgat gcgaccggaa tggcgctcca gagggctgtt tctcggccct ccggttcgcg
     6601 tcctgcacgt ggtcacgcgg ttgcacggaa agccgtccat gagcgatctt cgcatccttc
     6661 cgatccggac actgggacac ggtttcatcg aggaacccta cctgccgccg ggcaaagatg
     6721 agtattacct gcgcgatcag cagtgcccga gtcggccgga ccggtatcga agcctccggg
     6781 ccgacgagat cgaggtgctg attcgcaacg gcaatacggc cgacagttgg gacaacatcc
     6841 gtgtcaccga gcagttcgcg ccgcaccgga tcaagcggtg cgagttttac gggctggtgc
     6901 gaatcgggcg gcttgaggaa atgatgctcg aacatcacga catgcgcgtg cccgtgggaa
     6961 taaccgacag ccgcatcatc gcctgcgatt tcggcgatga cgttgccgtc catcacgtgc
     7021 ggtacctggc ccattacatc atcggcgacc actgcattct gttgaacatt gacgaggccc
     7081 acaccaccaa tcacgccaag ttcggcaacg ggattgtcaa ggaaggagaa tcggaagaga
     7141 tccgggtctg gatggacctg atcaacgagg ccggaggccg ccgcgtgatg cccttcgatg
     7201 gcatgacgac cggcgatgcg tacttgtggt cccgttaccg cgaccgaccc gcgctgatga
     7261 cccgctttgc cgagatcacc cagcgcatgt tcgacacgca ccggggtatc tacggaacga
     7321 tcggcaagga ctgcgtcatc aagaacaccg ggatcatcaa ggacgttaag atcggcgact
     7381 gctgctatat caagggggcc aacaaactca agaacctgac catcaactcg accgaagcgg
     7441 agccgactca gatcggtgag ggtgtcgaga tggtcaacgg cattgtgatg ccgggttgcc
     7501 acgtctttta cgggtgtaag gcggtcaggt tcatcatggg ctacaatagc aacctgaaat
     7561 acggcgcccg gctcatccac tcctatctgg gagacaactc gacggtttcc tgctgcgaga
     7621 tcctcaacaa cctcgtgttt cccgcacatg aacagcacca caacaactcg tttctcattg
     7681 cctcgctggt catgggacaa agcaatatcg cggcgggcgc caccatcggg tcgaatcaca
     7741 acagccgggc gcccgacggc gaggttcatg ccgaacgcgg cttctggccg gggctttgcg
     7801 tcagcgtcaa gcacaactgc cggttcgctt cgttcacgct gctggctcgc ggcgattacc
     7861 ccgccgaaat ggacattcgc ctccccttct gcctggtgag caacgacccg ggcggcgacc
     7921 ggctgcggat cacgccggcg tggtggtggc ggcataatat gtacgctttg gcccgcaaca
     7981 cgtggaagtt cagagctcgc gacaagcgta agtgcaaatg ccagcacatc gagttcgact
     8041 atctggcccc cgacaccgcc gaagagatct tcacggcctt gaggctgctg gagatgtggg
     8101 tggccaaggc cgcgttgcca agccggggcg aatccgccga acacatgtcg ccggacgcac
     8161 tcgccgagac ggggcggcgg ttgctcgtgg aatcgcccga cgaggtcgac aggcttgagg
     8221 ttctggccga aggagccgag aaatcctcgc ggaaggtcgt tatcctgaaa cctcgccagg
     8281 gttacctggc ctaccgggac atgcttcact attacggtgt gcgaaacctg gtcgactacc
     8341 tgatggccga tccacaggcg accctggcgt cggcggccga ggcactggcc ggcccgcagt
     8401 ggcggaactg ggtgaacgtc ggagggcaga tcgtccgggc ggatgatctg gaaaccctcc
     8461 tttccggaat cgagcagggg ctccaccaga catggcacgc catccaccgg gtgtttgaca
     8521 gttggtgggc caagtacccg ctggataagc agcaacatgc cttcgcctgc ctgaccacct
     8581 tgttggggag caactcaccg tcgaaggacc agtgggacgc tgcactggac cgcgcggtcg
     8641 agattcagca ctacattgcc gacgcggttt tcagctcccg caagaaggac gacgaagacc
     8701 ccttccgccg aatggtctac cggacgccgg aggaaatgac ggcggtcctg ggccgggccg
     8761 aggataacag cttcgtcaga caggtgcgcg aggatacgca cgcattcatt tcccgaatcg
     8821 aagagatccg ccggcgggga taatcgcctc accatcgcga gatgggagca actgcggcgg
     8881 cttggccaac gccgccggaa caggcttcat ccaggactgc tcgtggcctg atcgcgcggc
     8941 ggaacgatca gcaccctgaa tggcaatcgc ttttcttcca cttggctcaa ggcgccggcc
     9001 cgccggtgtt gaccaggccc ttacctctcg accgtccgga acagtcgtcc atcgcccggg
     9061 tcgagtctga ccttcacttg gcggtccgcg ggcagggttt cgccctcgga ccacttgccc
     9121 gtcttgcggt cgagttcctg aagcttgcgc ccagggatcg cgatcttcaa ctccgcctcc
     9181 gccggttctt tgtagctgcg attcacgatc atgaagctcg ttgttcgtcc ggacgcaccg
     9241 aacaggccga gcacgaactc gcctttgccg acaatctgca ccggcgagtc ggccggaaca
     9301 gcctgcgttc cgtaaggcag cggggcggtg tggtagacac ctgtcgaatc gagttgcagc
     9361 aaccacgggc cgagcttggc catctcgccg ttgagctcgg caaccacttt ggccagcggc
     9421 gagggcttgc cgtcctggta gatcccgccg tagaccttgg gcatccagta gacgaagtag
     9481 ctgatcccgc gccccccgta ggccatggtt gtgtatgcca gccagcgcat ctcggctgcg
     9541 ttgggcagcc gccacacctt ctcgccgttg caggcctgaa cgatgttcag gaacggcttg
     9601 ttcgcctcct gggccgcctg ccggatcaac gcgaggttca ggaaatactg gctgccgtcg
     9661 ccggtgagca ggaagtggta atggtcgtaa ctgatgagat ccggcttgac gatctcgatg
     9721 aacgccttca ggtgctcacg gtaggccagc acggtcttgt cgctggtgcc gacgccggca
     9781 aagttctgcg ggtaacctac cttggcccgc tcggcagcgt ccgcgctgac gccaagctgg
     9841 gcctcggtgg cgtaggtggg gaagaggttg atgtatgcca aatggtcggg atcccgctcg
     9901 cgcaggtagg caaccagtct gcccaagccg ggaaacgccc ccgccccggg ttcgtcggta
     9961 atgtagtacg cctcgagcgc cgggtgtctt ttcacccgct cgatcagcgt gtccagttgg
    10021 gcgcgcttgg ccgggtcgtc gagcacgtcg gggctcaaca gattactggt cagcatggcc
    10081 cgcaggccgt gtctggccgc cacgtccagg ccttcgaccg gcacccaggt caggttgtag
    10141 ttctccgcag cgacgttcgc caacgcctca tccgtcgccg gcggcgggca ccagaacgtg
    10201 atgatgaact gcttctgttt ccaacccttg gcggccgggg gctctgccgc gccggcagca
    10261 attgacagcg ttgcgagaag tgcagatgcg atgattgtct gtccgtacat ggaagatcct
    10321 cttcttatgc ccgacggtct atcacagagg gccgacgagg gcgtcggccc ctctacaggc
    10381 cgttcatgcc ctgtcgcgtc acggacgcgc atcctcaaca acgtttcacc gcatcacgat
    10441 tgccgagacg gacgaccttg ggctcaccga ggctcgacgg ccacggcagg ctgtacggga
    10501 tacccggcag caggtcgaag cagttgtcgg ccagcggggc ttcgccgtcc acgtccaggc
    10561 agacgcccca ggcgaacgcc tttgacgaca gcgtcagaat cccatccttg acggacattt
    10621 cgatcctcgg ctcgacgaac ttgaggtcct tgaaacgctc gatgaacagt cggtgctggc
    10681 tgaccatgcg gccgtctttt gagaggacag cgaatgcccc cgctgaagcg gagccgagtt
    10741 gctgccactt gttcttgtcg atggtcgcca gcacggtcga cgcgttggct ggcaaggtca
    10801 ccgtcttgcg tttgtcgtcc tccaggccgc cggccaggcg gaagagcccg ctgcgcagct
    10861 tgcccgacca ctggctcgtc gaatcgttga cgccgaagac caacacctgg tctttgtcct
    10921 cggccacgac cacccgcacc ggttgaaacg cccgccgaac ggggtgatag gcgagcttct
    10981 ttcgcaggta gtagtccacg atcgtccaac cgtgggtcac cggccaggaa tcgttgtaca
    11041 tccagaaaat ggccgacgaa ctgctgaaca tccggcggcg gtagttggcg atgtactcct
    11101 gcaagccctc ggcctgcatc agggcgctga cgtaggcata gtcctcccac tgcatttctt
    11161 ccgggttgag cccggtccac atctcgaccg tcgcgtaggc ccggccgaga tcgccgggag
    11221 tcggggcgtt gcacgcaaac gggttgtcgt gatggtccca ctccggcgac agcaggtatc
    11281 gttggtcttc ggacaggaac tgccgcagcg tcgccggcga agaagcaccg atcacgccgc
    11341 cctcgttcgg aaagcgatcc acgtattggc ggtatttcca gaagtccgcg ccgtgcggtt
    11401 cgaggatcga cacgccccag ggatgctgat cgccgaccgt ggggtcattc ggttccatgt
    11461 aatccggcga gtagggcgag ctgatccagt aggccgtcgc cgggttctcg caaaccgcga
    11521 tcttcggaat gtcatggtga aacaacgcgt agtgcgggtg cgtgcggtgt ttgttgtcgt
    11581 acccccagtt ccagtccccc cactcgatct cgttgttgcc gcaccaaacg accaggcacg
    11641 ggtgatgggc cagatcgcga accgcccagg ttacctcgcg acgtacttcg gcggcgaaat
    11701 cgggatggtc gcccggatac ttggcgcagg caaacaaaaa atcgtgccag atgagaatcc
    11761 ccgcttcgtc gcaagtttca cacaaagcat ggtcagcgaa gcaaccgccg ccccaaacac
    11821 gaagcgtgtt gaagtttgct tcgaccgcca gtcggaccag gtcgcgatac cgttgcggcg
    11881 tcaccctgct gtacagcagg tccggcggta cccagttgcc gcccttgcag aagatcggac
    11941 gattgttgat ccggatgatg aagtggcggc cctcctccgg gtgcggcgat tggtcgatct
    12001 ccacgcgacg cacgcctgtc ttgcgggtga ctgcctgagt ctcgcccgaa gcggtcagct
    12061 cgacgtcgac ggtatagcga gactgctcac cgtggccgat cggccaccag agccgcgggt
    12121 cggccaggtc gatgatgagc tcgtgattgc tctgacccgg taccatgcga accttcgcgg
    12181 agatatcctg gccggtctcg cggatgcgga cccgcaatgt gccttcgacc tccgagtcgc
    12241 cccgattgaa cacaaatgcc cgggcgtgga ccttggccga tcgcagatcc ggcgacggga
    12301 tggcaaaaac cgccgcctgc tccaggtacg gccccgcgtg ccattccagg cgaacatcgc
    12361 cgagaatccc gacgttcatc agccgagcat tccaatccca cccgcactgg tactgcggct
    12421 tgcgatgcca cggccgcttg gtcaacaagt cgatgaggtg acagtgatac tcggcaccgg
    12481 gcttgtccgc ggcggagtga atgcccgtgg aaagcttcac gatcagaaga ttgtcgccct
    12541 cctgcaacct gccggtcaca tcgaagcgag ctgggcggtg agcattcgca tgatgaccaa
    12601 tctcctgccc gttaagccac acaaccgcct cgtactccag cttgtcgaac accagccagg
    12661 cgtgttgttt ggtcgactct tcaggagcaa caaatgaccg gcggtagatc cagaactgct
    12721 cctcgaccca gcgggccttc agcgagttca acccgacgtt cggatcgtcg atcaaccccg
    12781 cgttcatcag gacggtgtgg atgggagccg gcacagcggc atcgagcatc ttccgtccgc
    12841 gcaacgtggg ggccgtgtag tggtccgggt tcatgagccc ggagccttcc gcccaggtca
    12901 ggccccatct cccattcagg tccatcgtgc caggcatgac tctcccctaa ggcggaccgc
    12961 ctttttgcgg gccgcaaccc aagtgctctg cgatccagaa acatgcgagc aagccgcaca
    13021 ggtatctggc cgtaaggttc taaccccgcc ctcggcatac gtcgaatcct cgtcggcgcg
    13081 gcgtcctcca cgcgggaaac aaacgaggaa cctgcctcgg acgctggata aagggtatag
    13141 aatctactcg tttctctgac gaacggcaaa caacagccgc ggctataaaa caccgggtac
    13201 gttggtcact tgcaaggcaa ggcctaatat ctggtatacg agtacgccgc tgacagcccc
    13261 cggcaacgcc tgggtcgccg ccgcaaggct ggggtgtccg ccactgacca ccgcatcggt
    13321 ggcggagtct gccggatcag cgcattcttg tggtccgcgt gctttcgcgc agcgtcccaa
    13381 ggcggccttc ggccgcaacc gaagcacccg gcgatccgga aacgtgcgcc caagccgcgt
    13441 gctttcccgg ccgtaaggtg gtagttgccg gtctcggccc agccgtgcaa accgcgggac
    13501 gatgatcctt gccgaggtac gatcatgatt gtcggtgtgg tgaaggaaag ctttcccggc
    13561 gaacagcgag tcgctctggt gccgtcatcc gtcaccgctc tgatgaagag tggttttgag
    13621 gtcatcattg agacgggcgc gggactggcg gccggttatc ccgattcgca gtatcacgaa
    13681 aagggaacca aggtggtgcc gactcgcggc gaagtcttcg caggttgcga catccttctt
    13741 caggttcgga cgctcggggc aaatccccgg gccggacggg ccgatctggc cttgctgcgg
    13801 cgagatcaaa tcctcatcgg tctggccgag ccgctgatgg ccgctgaggc aattagagcc
    13861 ttggccgaaa gcggcgtcac gctgttggcg ctggagctca tgccccgcat cacgcgggca
    13921 caaagcatgg acgtcctctc cagccagggg agcatcgccg gatacaaggc ggttctcatg
    13981 gccgccgaca cgctgggaaa agtctttccc atgatgatga ccgccgccgg aacaattcag
    14041 ccggccaggg ttctggtcat cggagcgggc gtggccgggc tgcaggccat tgcgaccgcc
    14101 aggcggctgg gcgccgtcgt cagcgggtgc gatgttcgac ccgccgtcaa ggaccaggtc
    14161 gaaagtctcg gggccaggtt cgtcgcactg cccgtcgaga ccgcggggac cgaaacgaaa
    14221 ggcgggtatg cgaaggaaca aaccgaggag cagctccgca agcagcgcga gctgatggcc
    14281 agggtcgtgg ccgaaagtga cgtggtgatc acgacggcgg cgatccccgg taagaaggcc
    14341 cccgtgttat tgactgcgga catggtcagg aggatgcaac cgggttcggt ggttgtggat
    14401 ctggccgccg agcggggcgg aaactgcgaa ctgactcagc ccgaccgcac ggtggtggac
    14461 aacggcgtga tcatcatcgg ccccgttaat cccgcgtcat tggtaccctg ccatgccagc
    14521 cagaccttct cgaacaatat ggtccagttg cttcagcacc tgatcgccaa ggacaaacag
    14581 gtccggatcg ataccggcga tgaaatcacc cgcgagatgc tggtctgtcg cgacgggcag
    14641 atcctgcatc cgcgcgtccg ggaagcgatg ggcttgacgc cgccggccgg accggaaagg
    14701 agtgatggct gatgctgatc atgctgttga ccattttcgt gttggccgtg ttcgtgggct
    14761 tcgagatcat caccaaggtt ccgccgacgc tgcatacgcc gctgatgtcg ggctcgaacg
    14821 cgatttcggg catcaccatc gtgggggcgg tgctggcggt gcatgccggc catccgattc
    14881 tcagcggagt catcgctttc ctggcggtca ccctggccat gatcaacgtc gtgggcggct
    14941 atctggtaac ccatcggatg ctgcggatgt tcaagaagaa ggggtagcac ggtgtctgat
    15001 cccacactca acctcgttta cctcggcgca tcggtgctgt tcattctggg catcaagggt
    15061 ctgacgcacc cgcggacggc ggtccggggc aacttcatca gcgccgtggc catgctggcg
    15121 gccgtcgcgg tcacgatcat cttcggccac ccgcacgggc tgccgatcgt gctggccggc
    15181 cttgtcgtcg gggccctcat cggcgtgatc ttggccgtca aagtgcggat gacccagatg
    15241 ccgcagatgg tcgccctgct gaacgggttc ggcggtgcgg cctcattgct ggtggcgtgg
    15301 tcggcgctga tcgaaaaggc ggcgacgcgg gcggcggcaa ctcagccaac cttgcctcct
    15361 cccgaaatgg cagcggtcgg cgacttgcag atgacgattg cgacggcagc gtcaggcctt
    15421 atcggcgcag tcaccttctg gggctctttg gtcgcgttcg ctaaacttga ggaaatcagg
    15481 cggttcaata aacccgtctt cctcccggct catcagttga tcaacttcgg tttgctcttg
    15541 ccggcggtcc tgctcggcgc ctggatcgta tggcagcccg aacacggcat gtggatctat
    15601 ttcctgttgg tcgccgtcgc gagcgcgctg ggcgttacgc tggtaatgcc gatcggcgga
    15661 gccgatatgc ccgtggtcat tgccctgctg aacagctact ccggcctggc cgcggcggcc
    15721 accggctttg tcatcgacaa caatatgctc atcatcgccg gctcgctggt cggagcctcc
    15781 ggcatcatcc tgacacgaat catgtgcaag gcgatgaacc gatcactggt caacgtattg
    15841 ttcggaaggc tggggccgac agccgcgacc gccggcgccg atgacgttta cggaggcagg
    15901 atcaagagca cgactcccga ggaggtggcc atgctgctcg agggcgcccg gcgcgtggtg
    15961 gtggtgcccg gttacggcat ggccgtggcc caggctcagt acgccgtccg cgacctctac
    16021 caggaactgg aaaaacgggg cacgaccgtt gagttcggca ttcatccggt cgccggacgc
    16081 atgcccggcc atatgaacgt gctgctggcc gaggtgaaca ttccttacga ccgcctcaag
    16141 gagatggatg agatcaactc cggctttgaa cagaccgatg tcgtcatcgt gctgggagcc
    16201 aacgacgtcg tgaacccaga ggctcgccac aaccccaaca gccccatcgc gggcatgccc
    16261 attctcgatg tggacaaggc ccgcaccgtc gtggtcatca agcgtagcct cagccctggc
    16321 tttgccggca tccccaaccc gctgttcgcg atggacaatt gcctgatgct cttcggcgac
    16381 ggtaaaaaag aggttgtcgc actgatcgag gcgctgaagc ggcaataaat ggatgacggg
    16441 cgacgcacgc gcgggtggtc cggattctcc tgtgcgtgtc agcgcacgct ctgccgccaa
    16501 gctgataatc atctagcaag cctccgagag gtatgctgtt ggttcgtgtt cag
//
LOCUS       DAOTPW010000125        16551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3479_length_16551_cov_5.860512, whole genome shotgun sequence.
ACCESSION   DAOTPW010000125 DAOTPW010000000
VERSION     DAOTPW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16551
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3479_length_16551_cov_5.860512"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    192..291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(499..1419)
                     /gene="rbsK"
                     /locus_tag="P5316_12350"
     CDS             complement(499..1419)
                     /gene="rbsK"
                     /locus_tag="P5316_12350"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRR85790.1"
                     /translation="MAGSICVVGSSNTDMILQMDRIPRPGETILGGEFSMAAGGKGAN
                     QAVGAARAGGQVVFVARVGNDMFGDQAIRGFMRDAINVDYVVRDPQAPSGVALIFVAK
                     DGENSIGVASGANGRLSPADVAKAREAITSADILLMQLETPLDTVKAAAEMAAAAGVR
                     VILNPAPAQPLGDDLLKHVSILTPNESEAELLTGVKVEGTKGAAEAAKVLMSKGIQTV
                     LITLGPTGTFVAAKDSAELVPSFKVKAVDTTAAGDIFNGALAVALAEGRALKEAVRFA
                     NAAAALSVTKLGAQPSAPRRQDIEQFMASA"
     gene            complement(1428..2438)
                     /locus_tag="P5316_12355"
     CDS             complement(1428..2438)
                     /locus_tag="P5316_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HRR85791.1"
                     /translation="MFVVESYAVAVVMCVITMLCWGSWANTQKLASKEWRFQLFYWDY
                     SIGVLLLTIIFALTLGSMGSAGRTFLADLGQASGTAIGLAFLGGVVFNLANILLVAAI
                     DIAGMSVAFPIGIGLALVEGVIVNHIRDPKGDPRFLFAGVAGVVIAIIIDAIAYGKLP
                     REGQKTLGKGIALSVVCGLLMGLFFYLVAAAMPPIAEINEPAQAGKLTPYSALFFFSL
                     GLFLSNFLFNTLVMAKPFVGEPVPLGDYFKKGNPRLHIVGILGGIIWSIGMSFAIIAG
                     DRAGYAISYGLGQGATMIAAFWGVFIWREFKTAPKGTNGLLALMFVFFIAGLSLIVYA
                     NA"
     gene            complement(2670..3362)
                     /locus_tag="P5316_12360"
     CDS             complement(2670..3362)
                     /locus_tag="P5316_12360"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRR85792.1"
                     /translation="MSKPQRLNETDKGAGQAPWIRHSDSGLLPSLGFPHSGFSGRTLE
                     APYTQAHGSHGHTLEAANLLPAHLLDGNEIVILAIKPSLWFVVFVSFRWLMAALALIL
                     AAGWLAELAPQVNAAMIIKAAAILAAGRVALAVLQWASRLYVLTNRRIMRLTGILNVD
                     LFECSLTKIQNTYVTLTWYERLTRLGTISFATAGTGGVEASWINVNNPLELHERVRSA
                     IHRAQRPGGSGI"
     gene            complement(3391..3993)
                     /gene="hpt"
                     /locus_tag="P5316_12365"
     CDS             complement(3391..3993)
                     /gene="hpt"
                     /locus_tag="P5316_12365"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRR85793.1"
                     /translation="MHPDIARILISRDKIAQRVKELADEIARTYEGDADAGITVVTIL
                     SGALIFVADLMRQLPLKMKVGLIMVSSYAGPRTSPSKPKVIHGLNTDVAGRHVLLVDD
                     ILDTGGTLRMVRRRLEKAGARSIRTCVLLRKPAKAPPDVGADFIGFDIEDAFVVGYGL
                     DFDDQYRNLPDIAVLREEIYARRNSGAAEMTDSQAPITNE"
     gene            4082..5239
                     /locus_tag="P5316_12370"
     CDS             4082..5239
                     /locus_tag="P5316_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HRR85794.1"
                     /translation="MAKRKEPQIPTPFDAAFEFAGQQVRRLVERYPDQFVAHTVQGRW
                     GIDPSAWSGSFNGLLPGMMWILHEVSGEAWWAETAERYSRAVEPLKALPQVGLGLVFY
                     HGSHRRWHEATVRAAAPQARVVEVMQEAARTLAGRFSKSARCLIMDPAAPVLAIEDLM
                     NVPLILHTADLSDDDWLLTIGSQHVATSRRHLVRGDGSTVGFALMGDHRECTIRADRA
                     GWQQDSCWARGQAWAVYGFATCGRLLGFGPWLETARQCAYYLIEKLSGDPIPPWDLDA
                     SPGLALPRDSSAAAIAAAGFLELAQAEQTVGPEQARQRQYLQDAALRILTALCEPEYL
                     AIDDPNWEGILKHGVGDLEKGLAVDESVIWGDFFFIEALYRVRRLLRAKRH"
     gene            5485..7836
                     /locus_tag="P5316_12375"
     CDS             5485..7836
                     /locus_tag="P5316_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85795.1"
                     /translation="MTGQRKNGLLPACCVAAAIAALWWPTVAMAVHLTGRIISVGFSS
                     TDPYGQDHGRCHYKLGRWTPIRVDLTNHDGDLFSGWLEVRQPDRDGDQVVARRQISIR
                     DNRRAFLYVPAARGARDVSFNVRVVSAEGSLIPLFNEQNEKMLELAPEDQIIPVNPRT
                     LVILDISEGGLNHLRNLTKETTSDRDLIVARCAPAELPDLVAGLEMVDVIVWDGADPS
                     ASIMDLGQREALIEWVRRGGRLVLGVSKNWDIINRSAFGEMLPARLSEVTSTTSPKVF
                     SKHACELLFGNAEPAISSLRSPLNFCPIARGALLPDAESIIPFEKAESVEENILAASR
                     PYGRGRVVLVAAEMRELFQVGSYSESFLRQVVRIRPAPAPRSTPQGGFGGSGPMRDID
                     LHGLVEEATDFDVAASLYFLFAFLFVVAYIVVATFGSWTWLKKKRLVRMSWPAFAAVS
                     VAASTTSLLAVRFVRGIGNSVECFTVVDGKAGSPDVGAVCYLGMKTATYSNVDLGIPG
                     NWLDIENSPPIPAAIRPRWYEELYPTGESVYAAGAEYIAAPVAGQLHDVLFRATAKDF
                     EGHWKGTMAGRLDAALYRDAGTWTELSGESWIENKLGADLDTCWLFIAARGFSENETR
                     DNRIYCYHFKVLPNGKRITVESVLKGTERLFSSRATEWARLFGYGRDAYYYGGYAQRE
                     EEKIDVRSDKFTSGMLLLTFFDEINQADEGFNRLDRSQGRVLDRSSELTRDTALFVGF
                     ADDPGPARLCWRRSGSNEKWRPIEPDHARTMYRFTIPIRGTRG"
     gene            7873..8799
                     /locus_tag="P5316_12380"
     CDS             7873..8799
                     /locus_tag="P5316_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR85796.1"
                     /translation="MIETINLTKRYGDLVALNNLHLNIEEGECFGYIGPNGAGKTTTI
                     RILATLLQPTWGEARICGHVVGYQSRKIRPLIGYVPDFFGTYADMVVVEYLEFFASAY
                     GITGHQRTKIVGDVLELTDLSYKRDALVDSLSRGMKQRLSIARVLLHDPKVLLLDEPA
                     SGLDPRARIEIRELLKELHRMGKTILISSHILPELAELCSTIGILERGELLFHGPVGE
                     ITRRAKRGVRVQIRVPDKIDIAVDTLRRLDEVKNVQQDNGFVSVELKNEMHDFSFLAR
                     ALIEKNLAIQELREEEVNLETAFMRLTKGIVQ"
     gene            8939..10024
                     /locus_tag="P5316_12385"
     CDS             8939..10024
                     /locus_tag="P5316_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85797.1"
                     /translation="MMIPKRLRPGLLVLLAVSTAGAQQPGAGPGNADPMDRPTEAGFR
                     FTPGMARIFASAWTGHLTGQLGLDKSKADEAAEKIARRFMQMMHQIDGPGQELIERFM
                     EEQMSAAARGEQSFIPRGFGKEFGERVVPLVPAIRDMARGTVQDIRPMLPLKQQLKLA
                     GEMMAFNKALDAFEERMRQWADGRADTTRNPFEDPDQEIRLDEQGQSPDLKRARQQAQ
                     IAIDREPTAAWEKYVNDAKTFYGFDESQSATADSVLRECLERADRIRADQQWRAKSHR
                     NRVWQSMMFRLPNGWNHPLRPLLDQEQTDLNSGFRAIENDLKTRIDRIPTPSQRRAAE
                     ERMESLLSEKGFDLKPANATAPEGGAQ"
     gene            10021..11172
                     /locus_tag="P5316_12390"
     CDS             10021..11172
                     /locus_tag="P5316_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85798.1"
                     /translation="MTRLTLILTSAFAVLVGPALGQDDEITPDEATQLETLNQQPSVS
                     QAPGEDVMRPTKHGVRLTPGLARAFAGVWIRETLERDIGATLSDEQKRTLSETIARKM
                     MRMGHTHGGKVAPLLEFAVENMGPGQRNIKPDKAREFAEKAKETIPVWRELFSSMTDD
                     CRPYLNEEQLKEIEKKQREIERALDKFDERMDRWARGEVKEGEGLFDDLENALEDESV
                     DGRESRGKPRKSPEVRSAEQRAVWSTRDLDPANWIEFLNQVRQTFKLTDEQYARGREL
                     LKDYTARAKALATPEWREKVRRNRALHGLGVTMYKESPAPWLFHLDQEYNEMAKPVLE
                     LRKAFQLEVMGLVTWEQREAVLAELREFGEKHGMTMDEADIRFPTTQTQ"
     gene            11198..13057
                     /locus_tag="P5316_12395"
     CDS             11198..13057
                     /locus_tag="P5316_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRR85799.1"
                     /translation="MAEVTRKILADVGLWLWRLVPANPIVLRVVSMGSKRASHFWART
                     GYLLVMLFVVLIVAQTVKHGESLGELAKSSASTFEAISVLQLAMMCFLAPVFTAGAIS
                     QEKDAETFNVLLTTPLTNAQIALGSLMSRLFFVLSLLLSGLPIFCITMLYGGVTTRQI
                     FISFGIAACTALVTGALAITISMIRVGTRGTILSFYAGIAVYLFATLALGLWPRTHVP
                     ESILPGSRTGMSWLSAFNPFLALLVGLKRVPPPAPALVDHYGWALSWVASAPHMAYMA
                     ITFGASVVMIALATVFVRRGVKQAEGSLLQRAVLRVLRRKSTEGERRRRPRHVWANPV
                     AWREAVTQGSAAGNRLVYYGFIFAGISAAVVLLVFHNSGRLANPGATAEQMANAARDW
                     LTGIVMVEFVTAILMALNTGATAISRERESGTMELMLVTPLESDYIIRGKIRGLISFT
                     APLMAVPAGTVLLMAVNDLVQGSDPPVVNLMSAILLPALLLVYSALACVVSLQTSLKS
                     KGSVQAIIQSMGIMTLVGFGLGVCAYGALETMEQIAGITGPLTFVMSIYMVLNPHQMT
                     SDRYSRFGATEAEVMAYLFVGTVIAIFLYGGIVTGMYRSMVRNFDMIVRKQSK"
     gene            13207..15633
                     /locus_tag="P5316_12400"
     CDS             13207..15633
                     /locus_tag="P5316_12400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRR85800.1"
                     /translation="MCRKLARGTVLVGLAVTFIGRPAESGSITGRREDQYGDGVHESL
                     IWAVGVGNNPSGAHGIALAWWGNFSINNLPAGQYYLAGNAHGVAPHAILDSPITVPAW
                     GSVEVNLRDRITMNSQGLTDLDSCRWAAQTFIATGRGLDEVAVISPDGGSRVSISVRE
                     DVPEGRQIGPARIVTNGSLFPAGARWLPGEVPLIPGRRYALRLDGVGGSTWTPAVAYR
                     PHGYPNGHAWFDGVPVPEADLKVAISCLDTGFIEDYSVSNWWRSKTFRELLQTFIPQG
                     SELRVAQMMLAGEGGFVMRAGVHQWSGSYPPGNQVGVAKHAEMSKDLPQAFVWGPGEA
                     PLTPGQPYAVRFTRADGQPFAIYGDSDNYPEGQAYFDGVPDGGIDINGTFVTREADRG
                     EIVLSNLVMTPISGTEVRAIFETDVPTTATIACKTGSPPFDTIWPADQIARQLHDVVI
                     RHLQPSTTYDMWLLAHHPDRNVLSTRATPVVVTTRNEFAPFAGHIRSQTGPVAGAEII
                     LEERRLTTFTDSQGGFIFTNVPTGRQTLRIQAVGCASVVQDVDVTADGQGFADISTTS
                     YTNLLAGSDNNPMAGWTAFGSFNGEFDSGSYSVTARTGPKWMGYVANWPNMSQGEHGG
                     GIFRTMTVEPGRRYRFGGFIRTQAFGSDHDPIDGLAVARIGVDLTGGTDHHSPNVKWA
                     RMRFTSGQWVEQTVDFTAEGPVVTLFAHHKWEYFYETPIWYIAAFDDLWLGPARLAIP
                     DFDCDGDVDQEDFGRFQQCLSGPGNMQGRTDCTLARLDVDEDVDQQDMVIFLECLSGA
                     GQPTPASCNQ"
     gene            15687..>16551
                     /locus_tag="P5316_12405"
     CDS             15687..>16551
                     /locus_tag="P5316_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85801.1"
                     /translation="MWRQLCFMAVCVSFLGGGSAIAAGTVKGTGRYERIKGRPGNYVE
                     LYEWNLYLSPDSGFTRGERRRLGACQEYGSACTPGYYQIDSPAGWYTILVTQPIFYAR
                     PTVVTDVQLIDGQIVTVDPDLNPDYSCYHVADGAWSWSTTWYQTFIARGTSITRVQFM
                     LAGSNASSIRVSVHRDNGGAITSWPQVGPAKSAAAGTGDGWVGYRSGDVPTTPYERYA
                     IKLTGEGGTPPYDFGIRSRVEDGNGYALGQAYDAAGNPRSLDLLVTVFSDNDGTVIPY
                     IDMTPGNVEDLA"
BASE COUNT         3174 a         4862 c         5347 g         3068 t
ORIGIN      
        1 tcgtccaact cgccgccctc ttttgttgga tcacctgaat gtcaacacga cctagaccgt
       61 taggctgctc gactattagg ccatcagact cctgcgtcca aaaatgcgtg cgcttagccg
      121 gaaggctaca ctcttgtaga ccatgtaggg catggtcgga gccgccatag gcggcgcaga
      181 cctgccgatg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcacacat
      301 agcgcggcag gtctcggtcg ctgcggcgac ctcgaccgtg ccctaccaca aggcgagcga
      361 cggccgcgat ccctcgctcg actacactct ggtagaccat gcagggcatg gtcggagccg
      421 ccataggcgg cgcagacctg ccgatgcagc acacatagcg cggcaggtct cggtcgctgc
      481 ggcgacctcg accgtgccct acgcggaggc catgaactgt tcgatatcct gccttcttgg
      541 ggccgaaggc tgggctccca gcttggtcac ggagagtgcg gcagcggcgt tggcaaaacg
      601 cactgcttcc ttaagcgccc gcccctcggc cagggcgact gccagggcac cgttgaagat
      661 gtcacccgcg gccgtggtat cgaccgcctt aaccttgaaa ctggggacca gttcggcaga
      721 gtccttcgcg gccacgaacg tcccggttgg ccccaaggtg atcagcaccg tctggatccc
      781 cttggacatc agtaccttcg ctgcctcggc ggcgcccttt gttccttcga cctttacacc
      841 ggtgagcagt tccgcctccg attcgttcgg cgtcaggatc gacacgtgct tgagcagatc
      901 gtcaccaagg ggctgagccg gggccggatt gagaataacg cgcacgccgg cggcggcagc
      961 catctcggcc gcggccttca ccgtgtccag aggcgtctcc aactgcatga gtaggatatc
     1021 cgcggaggtg atcgcctctc gtgccttggc gacgtctgcg ggcgagagtc gcccgttggc
     1081 cccggaagcc acgccgatgc tgttctcgcc gtccttggcg acgaagatca aggccacgcc
     1141 tgatggggct tgcgggtccc ggacgacgta gtcgacgttg atcgcgtccc gcatgaaccc
     1201 gcggatagcc tggtcgccga acatgtcgtt gcccacgcgg gcgacaaaga cgacctgtcc
     1261 gcctgctcgg gcggcgccga cggcctgatt ggcgcccttg cccccggcgg ccatggagaa
     1321 ctcgccgccc aggatcgtct cacccggccg ggggatgcga tccatttgca gaatcatgtc
     1381 cgtattcgaa ctgccgacga cgcaaatact ccctgccatc gttcacctca cgcgttcgcg
     1441 tacacgatca gactcagtcc ggcgatgaaa aacacaaaca tcaatgcgag caatccgttg
     1501 gtgcctttcg gcgcggtctt gaattctctc cagatgaaga ccccccagaa ggcggcgatc
     1561 atggtcgccc cttgcccgag gccgtacgaa atggcgtaac cggcccgatc gcccgcgatg
     1621 atggcgaacg acataccgat actccagatg atgccgccga ggatgccgac gatatgcagc
     1681 cgtggatttc ccttcttgaa gtagtccccc aagggcacgg gttcgccgac aaagggcttg
     1741 gccatgacca gggtgttgaa caggaagttc gacaggaaca agcccagaga aaagaaaaag
     1801 agcgcgctgt agggagtcaa cttgcctgcc tgggcaggct cgttgatttc ggcaataggt
     1861 ggcatcgccg cggccacgag gtagaagaac aggcccatca acagaccgca gacgaccgag
     1921 agggcaatac ccttgcccag cgttttctgg ccctctcttg gcagtttgcc gtaggcgatg
     1981 gcgtcgatga tgatggcgat gacgactccg gcgactcccg cgaacaggaa acgcggatcg
     2041 cccttcggat ctcgtatgtg attgacgatg acgccttcga ccagtgcgag gccgatacct
     2101 atcgggaaag cgacggacat gcctgcgatg tcgatggccg cgaccaggag aatgttggcg
     2161 agattaaaga ccacgccgcc caggaacgcg agcccgatcg ccgtgccgct ggcttgtccc
     2221 agatcggcaa ggaaggtgcg gccggcggag cccatactgc ccagtgtcag ggcaaagatg
     2281 atggtgagca gcagcacacc gatcgaatag tcccagtaga acagttggaa ccgccattcc
     2341 ttgctggcca gtttctgagt gttggcccag gaaccccagc acaacatggt gatgacgcac
     2401 atgacgacgg ccacggcata agactcgacg acaaacatgt gtttgctcct tgttcagcgg
     2461 acgatagtgc tccggtaggc tacaggaact tgggggaaag tgaaagtggc cgggccaagc
     2521 cgactcgggt gggtggccat tcgggcggtt gtttgaggac cgccagcgca ggcgacgggt
     2581 cgagccgctg cggggcccat acggtcgcgg ttctgacaga acggggaccg cagtttctgc
     2641 ccgccggacg tgtcaccggc atggccggtt cagatcccgc tgccgcccgg tcgttgggcc
     2701 cgatgaatgg cggatcggac gcgttcgtgg agttcgaggg ggttattcac attaatccat
     2761 gacgcctcca cgccgccggt cccggccgtg gcaaaggaga tcgttcccag ccgggtcagg
     2821 cgttcatacc aggtcagcgt cacgtaggta ttctgtatct tggtgaggga acactcgaac
     2881 agatcgacgt tgaggattcc ggtaagccgc atgatgcggc ggttggtcag gacgtagaga
     2941 cgggatgccc actggagcac ggccaaggcg acgcggccgg cggccagaat agcggcggct
     3001 ttaatgatca tcgccgcgtt gacttgtgga gccagttcgg ccaaccagcc ggccgcgaga
     3061 atcagcgcca gagccgccat cagccagcga aacgagacaa aaaccacaaa ccagagagag
     3121 ggtttgatcg ccaggatgac aatctcattg ccgtcgagca ggtgggcggg caggagattg
     3181 gcggcctcga gcgtgtgacc gtggctgccg tgagcctggg tgtatggggc ctcgagggtg
     3241 cgaccggaga agccggaatg aggaaatccg agtgacggaa gaagtccgga atccgaatga
     3301 cggatccatg gggcttgtcc tgcccctttg tcagtctcat tcaggcgttg gggtttcgac
     3361 attcgtcact atctcgtcat tggggcttcg tcattcattg gtgattgggg cttgggaatc
     3421 ggtcatttcc gccgccccgc tgttcctccg agcatagatt tcctcccgca gaaccgcaat
     3481 atcggggagg ttacgatatt gatcgtcgaa atccaggccg tagcccacaa caaaagcgtc
     3541 ttcgatgtca aacccgatga aatcggcccc gacgtcggga ggggccttgg cgggcttgcg
     3601 taacaggacg caggttcgga ttgaacgggc cccggccttt tccaggcgac gtcggaccat
     3661 ccggagcgtc ccgccggtgt ccaggatgtc gtccaccagc aatacatggc gtccggcgac
     3721 gtccgtattg aggccgtgaa tcaccttcgg cttgctcggg ctggttcgcg gcccggcata
     3781 gctggaaacc atgatgagtc cgaccttcat cttgagcggc aactgccgca tgaggtcggc
     3841 gacgaagatc agcgctcccg acagaatcgt gacaaccgtg atgccggcat ccgcatcgcc
     3901 ctcgtacgtg cgggcaattt cgtccgccaa ctccttcact cgctgggcga tcttatcgcg
     3961 ggagatcaga atccgtgcga tgtccgggtg catgggcggc attctactcg attgattaca
     4021 tcttggcgct ccgctagtct ttccgcgact gccgaggtgg ccggatcgcg cgaggaatac
     4081 gatggccaaa cgcaaggaac cccagattcc gacgccgttc gatgcggcgt ttgagtttgc
     4141 cggtcagcaa gtcaggcgac tggtcgagcg gtaccctgac cagttcgtcg cgcacacggt
     4201 ccagggacgc tggggcatcg atccgtcggc ctggtcaggc agcttcaatg gtcttttgcc
     4261 ggggatgatg tggatcctcc acgaggtcag cggcgaggct tggtgggccg agactgccga
     4321 acgatacagc cgggccgtcg agcctctgaa agcactgcct caggtcggcc tgggcctggt
     4381 gttctaccac ggaagtcatc ggcgctggca tgaggcaacg gttcgggccg ctgctccgca
     4441 ggcgcgcgtg gtggaggtga tgcaggaagc cgcgcggacg ctggccggcc gcttcagcaa
     4501 gagtgcccgc tgcctgatca tggacccggc ggcgccggtc ctggcaatcg aggacctgat
     4561 gaacgtaccc ctgatcctgc acacggccga cttgtcggat gacgactggt tactcaccat
     4621 cggcagccag cacgtggcca cttctcgccg ccatttggtt cggggagacg gttcaaccgt
     4681 cgggttcgcc ctgatgggag atcaccgcga atgcacgatt cgggctgacc gggccggttg
     4741 gcaacaggat tcctgctggg ctcgcgggca ggcttgggcg gtttacggct tcgccacctg
     4801 cggacggttg ctggggtttg gcccgtggct ggagactgcc cgacaatgtg cctactatct
     4861 gatcgaaaag ctttccggag atccgatccc accctgggac ctcgacgcat ccccagggtt
     4921 agcgttgccc cgcgacagtt ccgccgccgc gattgcggcc gccgggttcc tggaattggc
     4981 acaggccgag cagaccgtcg gcccagagca ggctcgccag cgccagtacc ttcaggatgc
     5041 ggcgcttcgc atcctgaccg cactgtgcga gcctgagtac ttggccattg acgatccaaa
     5101 ctgggaggga atcctcaagc acggtgtcgg ggatctggaa aagggcttgg ccgtcgatga
     5161 gtcggtaatc tggggcgact tcttcttcat cgaggccctg taccgggtca ggcgtctgct
     5221 ccgcgctaag cggcactgaa aaacagggac cggttctctc gaaacaaacc aatgccgtgc
     5281 tcgtctgtat atcatgtggg agggatcatc tgcctgcagg cggaaaatcg ctcaggttga
     5341 gaagcgagcc ctcgctactg tacaatatgg tgtccgacga aaaccgcata cactgggagt
     5401 aatgcttggt cactcacaat gaacagatga tcgccggatt cgtagattaa agcagcaagg
     5461 catagacgag gtcgttcaaa cctcatgacc ggccagcgaa aaaacggact cttgcctgct
     5521 tgttgcgtcg cagccgccat cgcagccttg tggtggccca ctgtggccat ggccgttcat
     5581 ctcaccggtc ggatcatcag tgtcggtttt tcgtcaacgg acccttatgg gcaggatcac
     5641 ggtcgctgcc actacaagct cgggcgatgg acacccattc gtgtggacct gaccaaccat
     5701 gacggcgacc ttttcagcgg gtggctcgaa gtccgacaac cggatcgcga cggcgatcag
     5761 gtggtggccc gccggcagat ttctatacgg gacaacaggc gagcctttct ctacgtgccg
     5821 gcggctcgag gcgcccggga cgtgtccttc aacgttcgtg tagtttccgc cgagggctcg
     5881 ctcattccct tgttcaatga gcagaatgag aagatgctgg agctggcgcc ggaagaccag
     5941 atcattcccg tgaatcccag gacgctggta atcctggata tcagcgaggg cggtctgaac
     6001 catctccgca accttaccaa agagacgacg tcggacagag atctgatcgt tgcccgatgc
     6061 gctcccgctg aattgcccga tctggtggcc gggctggaaa tggtcgatgt gatcgtttgg
     6121 gacggggcgg acccatcggc atccatcatg gatctgggcc aacgcgaggc gctgatcgag
     6181 tgggttcgcc gggggggcag gcttgttctg ggcgtcagca agaactggga catcatcaac
     6241 cgcagtgcct tcggcgagat gcttccggcg cgtctgagcg aggtgacgag caccacctct
     6301 ccgaaggttt tctcgaagca cgcttgcgaa ctgctgttcg gcaacgcgga gccggccata
     6361 tccagcctgc ggtctcccct gaacttctgc cccatcgcgc gcggcgcgct tctcccggat
     6421 gcggaatcga tcattccttt tgagaaggcc gaatcggttg aagagaacat tctggcggcc
     6481 tcacggcctt acggtcgcgg ccgggtggta ctggtcgccg cggagatgag agagctgttc
     6541 caggtcggct cttattcgga gtcatttcta cgtcaggtcg tcaggatcag gccggcaccg
     6601 gcgccgcgga gcacgccgca gggcgggttc gggggctccg ggccgatgcg cgacatcgac
     6661 ctgcacggtc tcgtcgagga agccacggat ttcgacgtcg ccgcaagtct ttattttcta
     6721 ttcgccttcc tgttcgtggt ggcgtacatt gtggtggcga cgttcggcag ttggacgtgg
     6781 ctgaagaaga agaggctggt tcgcatgtcc tggcccgcct ttgccgcagt gagcgtcgca
     6841 gccagcacga ccagccttct ggcggtacgt ttcgtgcgcg gcatcggcaa cagtgtggag
     6901 tgtttcacgg tggtcgacgg gaaagccggg tctccggacg tcggagccgt ctgttacctg
     6961 ggcatgaaga cggcgaccta cagcaacgtg gacctgggca taccgggcaa ctggctggac
     7021 atcgagaact cgccgccgat tcccgcggcg atccggccga ggtggtacga ggagctgtac
     7081 ccgacgggcg agtcggtcta tgcggcgggc gcggagtaca tagcggcgcc ggtggcgggc
     7141 caactgcatg acgttctgtt ccgggcgacg gccaaggatt tcgaaggtca ttggaaaggc
     7201 acgatggccg gccgacttga cgccgccttg taccgggacg cggggacgtg gacggagctg
     7261 agcggagaaa gctggatcga gaacaagctg ggcgccgatc tggacacctg ctggctcttt
     7321 atcgcggcac ggggcttctc cgaaaacgaa acccgtgaca accggatcta ctgttaccac
     7381 ttcaaggtcc tgccgaacgg caagaggatc acggttgagt cggtgctcaa aggaaccgaa
     7441 cggctcttca gttcccgagc cacggagtgg gcgagattgt tcggctatgg cagggacgcc
     7501 tactactatg ggggttatgc ccagcgagag gaagaaaaga ttgacgtccg cagcgacaag
     7561 tttaccagcg gcatgctgct gctgaccttc ttcgacgaga tcaatcaggc ggatgagggc
     7621 ttcaacagac tggaccgctc gcaggggcgg gtgctcgacc ggtcaagcga actgacgcgg
     7681 gacaccgctt tgttcgtcgg ttttgccgat gatcccggac cggcccggct gtgctggcgg
     7741 agaagcggga gcaacgaaaa gtggaggccc atcgaacccg atcacgcgcg aaccatgtac
     7801 cggttcacga tcccgatcag gggcacgagg ggatgaggac ggggtgcaag aaccaagcgg
     7861 gcctgagtga tgatcatcga gacgatcaac ctgaccaagc gatacggcga cctggtagcg
     7921 ctgaataacc ttcacctgaa tatcgaggaa ggcgagtgct tcgggtacat cgggccgaac
     7981 ggcgcgggca agacgacgac aatcaggatt ctggcgacgc tgttgcagcc gacctggggc
     8041 gaggcccgca tctgcggcca cgtggtgggt tatcaatcgc gcaagatccg cccgctgatc
     8101 ggctacgtgc ccgacttctt cggcacctac gccgacatgg tggtcgtcga gtatctggag
     8161 tttttcgctt cggcctacgg catcacggga caccagcgga ccaagatcgt gggcgacgtg
     8221 ctcgagttga ccgacctgag ctacaagcgc gacgctctgg tcgactcgct ctcgcgcggc
     8281 atgaagcagc ggctgagtat cgcccgggtg ctgctgcacg acccgaaggt gctgttgctg
     8341 gacgagccgg ccagcggcct ggacccgcgg gcccgcatcg aaatccgcga gctgctcaag
     8401 gaactgcacc ggatgggcaa gaccatcctg atcagctcgc acattctgcc tgaactggcc
     8461 gagctttgtt cgaccatcgg gatccttgag cggggcgaac tgctgtttca cggcccggtg
     8521 ggcgagatca cccgcagagc caaacgcggc gtccgcgtgc agatccgcgt gcccgacaag
     8581 attgacatcg ccgtggatac gctccgccga ctggacgagg tcaagaacgt gcaacaggac
     8641 aacggcttcg tctcggtgga gttgaagaac gagatgcacg atttcagttt tctggcccgg
     8701 gcattgattg agaagaacct ggcgatccag gagcttcgtg aggaagaggt caatctcgaa
     8761 accgcgttca tgcgtctgac caaggggata gttcaatagg aaccgttgcg gcgacctttc
     8821 cgggagtcgg tctcagaccg gcggagaagc cgcgggtggc gtcccacgga caggccgcca
     8881 acaacacccg acgtctgacc aacagaccgg ttcaacagca actgcgatag ggggaaccat
     8941 gatgattcca aaacgtttgc ggcccggact gctcgtgctg ctcgccgttt caacggcggg
     9001 ggcacaacaa cccggagccg ggccgggcaa cgccgacccg atggaccggc cgaccgaagc
     9061 cgggtttcgt tttactccgg ggatggctcg gatctttgcc agtgcgtgga ccggtcacct
     9121 gacgggccaa ctgggcctgg ataagtcgaa agccgacgag gccgcggaga agatcgcccg
     9181 ccgcttcatg cagatgatgc accagattga cggtccaggg caggaactga tcgagcggtt
     9241 catggaggag cagatgtcgg ccgccgcccg cggcgagcaa tcgttcattc cgagaggatt
     9301 cggtaaggaa ttcggcgagc gggttgtccc gctggtcccg gcaatccgcg acatggcccg
     9361 cggcacggtc caggacatcc ggcccatgct tcctctcaag caacagctca agctcgccgg
     9421 cgagatgatg gcgttcaaca aggctcttga tgcctttgaa gaacggatgc ggcaatgggc
     9481 tgacggccgg gccgacacga cgcgtaaccc gttcgaagac cccgatcaag agatcagact
     9541 cgatgagcaa gggcagtcgc ccgacctgaa gcgggcccgc cagcaggccc agatcgccat
     9601 cgacagggaa ccgaccgcag cctgggagaa atacgtcaat gatgccaaga cgttctacgg
     9661 ttttgatgaa tcccagtcgg cgacggccga ctcggttctc cgcgaatgcc tggagcgggc
     9721 cgaccggatc cgggcggacc agcagtggcg tgccaagtcc caccgcaacc gggtatggca
     9781 atcgatgatg tttcgcctgc cgaacggctg gaaccatcct ttgaggcctt tgctggacca
     9841 ggagcagacc gacctgaact ccggcttcag ggcgatcgaa aacgatctca agactcgcat
     9901 cgaccgcatt cccacaccgt cgcaacgacg cgcggccgag gagagaatgg agtctctctt
     9961 gtcggaaaag ggattcgacc tgaagccggc gaatgccacg gctccggaag gaggtgccca
    10021 atgacccggc tcaccttgat tctgacgtcg gcgttcgcgg tgttggtcgg tccggcgctg
    10081 gggcaggacg acgaaatcac cccggatgag gcgacgcaac tggaaacgct gaaccaacag
    10141 ccatccgtct cccaggcccc gggcgaagac gtgatgcgtc ccacaaagca cggggtccgt
    10201 ctgacacccg gattggcgcg ggcattcgcc ggcgtctgga ttcgtgagac gctcgagagg
    10261 gatatcggcg cgacgctctc ggacgagcag aagagaacgc tgagcgaaac gatcgcgcgg
    10321 aagatgatgc gaatggggca tacccatggc ggcaaggtcg ccccgctgct ggagtttgct
    10381 gtcgagaaca tggggcccgg ccagcgaaac atcaagcccg acaaggctag agagtttgcc
    10441 gagaaagcca aggagacgat accggtctgg cgagagctgt tcagcagcat gaccgacgac
    10501 tgccgaccgt atcttaacga ggagcagttg aaggaaatcg agaagaagca gcgggagatc
    10561 gagcgggctc tcgacaagtt cgatgagcgc atggatcgct gggcacgcgg cgaggtcaag
    10621 gagggagagg ggcttttcga tgaccttgag aatgccttgg aggacgaatc ggtcgacggg
    10681 cgggaaagcc gcggcaagcc caggaaatcg cctgaggtga ggtcggccga gcagagagcc
    10741 gtctggtcca cccgggatct ggatcccgcc aactggattg agttcctcaa tcaggtgcgg
    10801 cagactttca agctgaccga cgagcaatac gccagggggc gggaactgct caaggactat
    10861 accgccagag ccaaagcgct cgccacgccc gagtggcggg agaaggttcg cagaaaccgc
    10921 gccctccacg gtctgggcgt caccatgtac aaggagtcgc cggcaccgtg gctgtttcat
    10981 ctggaccagg aatacaacga aatggccaaa ccggttcttg aactgcggaa ggcctttcag
    11041 ttggaggtca tggggctggt gacttgggag cagcgtgagg cggtgcttgc cgaactgcgc
    11101 gagttcggcg agaagcacgg catgacgatg gacgaggcgg atatccggtt cccgacgaca
    11161 caaacgcagt aagcggtaag gtcgaggcgc tgagacgatg gcagaggtga cccggaagat
    11221 tctcgcggac gtcggtctgt ggctctggcg gctggtgccg gccaatccga tcgtgctgcg
    11281 ggtggtgagc atgggcagca agcgagcgtc ccatttctgg gcccgcacag gttatctgct
    11341 ggtcatgctc ttcgtcgtgc tgatcgtcgc gcagaccgtg aagcacgggg aatcactggg
    11401 cgagctggcc aagtcgtcag cgagcacctt tgaggcaatc tcggttctac aactggcgat
    11461 gatgtgtttt ctggcgccgg tcttcaccgc cggtgcgatt tcgcaggaga aggatgccga
    11521 gacgttcaac gtgctgctga ccacgccgct gaccaacgcc cagatcgccc tgggctcgtt
    11581 gatgagccgg ctgttcttcg tgctcagtct gctgctctcg gggttgccga ttttctgcat
    11641 taccatgctt tacggcggcg tcaccacccg gcagattttc atcagcttcg gcattgccgc
    11701 ctgtaccgcc ctggtgaccg gggcgctggc catcacgatc agcatgatcc gcgtcggcac
    11761 gcgggggacc atcctgtcct tttacgccgg tattgcggtg tatctgtttg cgaccctggc
    11821 gttagggctt tggccgcgaa cgcacgtgcc cgagtcgatc ttgcccggca gcaggacggg
    11881 catgagctgg ctgtcggcgt tcaatccctt cctggccctt ctggtggggc tgaagagggt
    11941 gccgccgccg gcaccggccc tggtggacca ctacgggtgg gcgttatcct gggtggcgtc
    12001 ggctccgcac atggcgtaca tggcgatcac attcggggca tcggtggtca tgatcgccct
    12061 ggcaaccgtt ttcgtccggc gcggcgtcaa gcaggccgaa ggcagcttgt tgcaacgcgc
    12121 ggtcttgcgg gtcctgcgga ggaaatcaac cgagggtgag cgtcgtcgtc ggccgcggca
    12181 cgtctgggcc aacccggtag cgtggcgcga ggcggtcacc cagggcagtg ccgccggcaa
    12241 ccgcctggtc tactacgggt tcatctttgc gggcatttcg gcggccgtcg tgctgctggt
    12301 ctttcacaac agcgggcgac tcgccaaccc gggagccacg gcagagcaga tggccaatgc
    12361 cgctcgcgac tggctcaccg gcatcgtgat ggtcgagttc gtcaccgcta ttctcatggc
    12421 tctcaacacc ggcgcgaccg ccatcagccg tgaacgcgag tcgggtacca tggaattgat
    12481 gctggtcacg ccgctggaga gtgattacat cattcgtggc aagatccgcg gcttgatcag
    12541 ctttaccgcg ccgctgatgg ccgtcccggc gggcaccgtc ctgttgatgg ccgtcaacga
    12601 cctggtccag gggtccgatc cgccggtggt caacctgatg tcggcgatac tcctgcccgc
    12661 cctgttgttg gtctattcgg cactggcctg cgtggtgagt ctgcagactt cgctcaagag
    12721 caaggggtcc gtccaggcca tcattcagag catgggcatc atgacgctgg tcgggttcgg
    12781 gctgggcgtc tgtgcttacg gggccctcga gacgatggag cagattgccg gcatcaccgg
    12841 gccgctgacc tttgtcatgt cgatctacat ggtgctgaac cctcatcaaa tgaccagcga
    12901 ccgctattcc aggttcgggg cgaccgaggc cgaagtcatg gcatatctct ttgtcgggac
    12961 ggtcattgcg atctttctat acggtggcat cgtcacgggc atgtatcggt cgatggtccg
    13021 caatttcgac atgatcgtcc gtaagcagtc gaagtaagaa gaactctgtg aaatggggcc
    13081 tgccaaggcg agtcggcgtc cgccgggttc gtgctccaca tttttccaca ttgggctatc
    13141 cgtatgatat aatgaccgtg attggtccga ctcggctcgc ggagaccgaa ctgcggagga
    13201 catcccatgt gtcgcaagct tgcgcgaggc accgtgctcg tggggctggc cgtgacattc
    13261 atcggccggc ctgcggagtc ggggtcgatc accggtcgtc gggaagacca gtatggcgat
    13321 ggtgtgcacg agtcgctcat ctgggcggtc ggggtgggga acaatccaag tggcgctcac
    13381 ggcattgccc tggcctggtg gggaaacttc agcattaaca acctgccggc aggccagtac
    13441 taccttgcgg gcaacgccca tggcgtcgct ccgcacgcga ttctggattc acccatcacc
    13501 gtcccagctt ggggatctgt cgaagtgaac cttcgcgatc gcatcaccat gaacagccag
    13561 ggcctgacgg acttggactc ctgccgatgg gcggcacaaa cgttcatcgc tacggggcgt
    13621 ggtctggacg aggtcgctgt gatctcgccg gacggcggaa gccgggtgag catcagcgtg
    13681 cgcgaagacg tccccgaggg acgacagatc ggtccggctc ggatcgtgac caacgggagc
    13741 ctattccccg cgggtgcccg ctggttgccg ggagaggtgc cgctgattcc gggacgtcga
    13801 tacgcgctgc gtctggacgg cgtgggggga agcacttgga cgccggcagt tgcgtaccgg
    13861 ccgcacggtt acccgaatgg tcacgcgtgg ttcgacggcg tgccggtgcc agaggccgac
    13921 ctgaaggtgg ccatctcctg cctggacacc ggctttatcg aagattacag cgtcagcaac
    13981 tggtggcggt cgaaaacgtt ccgcgaactg ctgcaaacct tcattcctca aggcagtgag
    14041 ctgcgtgtgg cacagatgat gctcgcgggt gagggtggct ttgtgatgcg ggccggcgtt
    14101 caccagtggt ccggcagtta tccgccgggg aatcaggtgg gagtcgccaa gcacgccgag
    14161 atgtccaagg acctgccgca agccttcgtg tggggccccg gcgaggcccc gctcacgccg
    14221 ggccaaccct acgcggtccg gtttactcgg gccgacgggc agccctttgc catctacggc
    14281 gattcggaca attaccctga ggggcaggcc tactttgacg gcgtgcccga cgggggaatc
    14341 gacatcaacg ggacgttcgt tacgcgggag gccgatcgcg gcgagattgt cttgagcaat
    14401 ctggtcatga cgccgatctc cggcactgag gtgcgtgcaa tctttgagac ggacgttccg
    14461 acaaccgcga cgatcgcctg caagacgggc tcgccgccct tcgacacgat ctggccggcg
    14521 gatcagattg cccgccaatt acatgacgtc gtgatccgcc accttcagcc cagcaccacc
    14581 tacgacatgt ggcttctggc tcaccacccg gaccgcaatg tcctcagcac ccgtgcgacc
    14641 cctgttgtcg tcaccacccg caacgaattc gcaccgttcg ccgggcatat ccgcagtcag
    14701 accggcccgg tggcgggggc cgaaatcatc cttgaagaac gcaggctgac aacgttcacg
    14761 gattcacagg gcggcttcat cttcacaaac gttcccaccg gccggcagac actgagaata
    14821 caggcggtcg gctgcgcgag cgtcgtgcag gacgtggatg tgaccgccga cggccagggt
    14881 tttgcggaca tttcgacgac ctcttacacg aatctgctgg ccggcagcga caacaacccg
    14941 atggccggtt ggaccgcgtt tgggagcttc aacggcgagt tcgacagcgg ttcgtacagt
    15001 gtgacggctc ggaccggtcc gaaatggatg ggctatgtgg cgaactggcc gaatatgtcc
    15061 cagggcgagc atggcggcgg gatcttccgc acgatgaccg tggagcccgg gcggcgatac
    15121 cgcttcggcg ggtttattcg gacgcaggcg ttcggatccg atcatgatcc gatcgacggt
    15181 ctggcggttg ctcgtatcgg ggtcgatctc accggcggaa cggatcacca cagccccaac
    15241 gtgaaatggg caaggatgcg gttcaccagc ggccagtggg tggagcagac cgtcgacttc
    15301 accgccgagg ggccggtcgt cacgctgttt gcacaccaca agtgggagta tttctacgag
    15361 acgccgatct ggtatatcgc ggcctttgac gacttgtggc tggggccggc caggctcgcg
    15421 attccggatt ttgactgcga cggcgacgtc gatcaggagg atttcggccg atttcagcaa
    15481 tgcctttcag gcccgggcaa catgcaaggc cgaacggatt gtacccttgc ccgactggac
    15541 gtggacgagg atgtggacca gcaggacatg gtcatctttc tggaatgctt gagcggggcc
    15601 ggtcagccga cgccggcgtc gtgcaatcag tgaggagcag cggcgaggac gctgaaacag
    15661 ggatttcgcg aatgggagac aagaacatgt ggcgacaatt gtgcttcatg gcggtttgcg
    15721 tctcatttct cggcggcggg tccgcgatcg ctgcgggcac ggtgaagggt accggccgat
    15781 acgagaggat caagggccgc cccggcaatt atgtcgagtt gtacgaatgg aacctttacc
    15841 tgtcgcccga ctccgggttc acgcgcggcg aacgaaggag attgggtgcc tgccaggaat
    15901 acggttcagc ctgcacgccc ggttactatc agatcgactc cccggccgga tggtacacga
    15961 ttctcgtcac gcaacccatt ttctacgctc gtcccacggt ggtcaccgac gtgcagctca
    16021 tcgacggcca gatcgtcacc gtcgacccgg acctcaatcc cgactattcc tgctaccatg
    16081 ttgcagatgg agcatggagt tggagcacga cgtggtatca gactttcatt gcgcgaggta
    16141 cgtcgatcac gcgcgttcag ttcatgttgg cggggtcaaa tgccagttcg atccgcgtct
    16201 cggtgcatcg cgacaacggg ggcgcgatca cgtcctggcc tcaggttggt ccggccaagt
    16261 ctgcggccgc cggcaccggt gacggctggg tcggctatcg cagcggcgat gtgccgacca
    16321 cgccgtatga acgatacgcc atcaagctta ccggcgaggg cgggactcca ccctatgatt
    16381 tcggcatccg cagccgggtg gaggacggca acggttacgc actcgggcag gcctacgacg
    16441 cggccggcaa tccccgaagc ctcgatctgc tcgtgacggt cttcagcgac aacgacggga
    16501 cggtcattcc atacatcgac atgacacccg gcaacgtcga ggatctggcg g
//
LOCUS       DAOTPW010000126        16366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3533_length_16366_cov_5.928882, whole genome shotgun sequence.
ACCESSION   DAOTPW010000126 DAOTPW010000000
VERSION     DAOTPW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16366
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3533_length_16366_cov_5.928882"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1032
                     /locus_tag="P5316_12410"
     CDS             61..1032
                     /locus_tag="P5316_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR85802.1"
                     /translation="MRCAGRLRGARPAADPDRRTAFTLIEILVVVAIVVLLTAILIPA
                     LSQARAQARRSVCLSNMSHLAKAVLSFSATHKDRGQLYADWDYVDAVDPDHSIYAYQN
                     GWFEDGVLKNPHKPYLKPWPVAYAGELGMPSVRNTHQYFLDETTYLKGYPDTEQPEYH
                     FRRFGKHEPLYCPADKFSIRETNSPEHVYGVLSYAINEDVFGARQGQCCTRSGWKDQA
                     LGIMSRIANSSEVAMFTDAGHETLERSQALKSDGSIRIRAGQWVPTWFRSAQGAAFLN
                     EVNAKVIVAVPIERHGKNGGISVAYADGHGSFVAGSGAWELSTGFPA"
     gene            1527..2045
                     /locus_tag="P5316_12415"
     CDS             1527..2045
                     /locus_tag="P5316_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR85803.1"
                     /translation="MFADFTKDAEAALIAQVKSNSRLFFRLAYGVVRDVQAAEDVCQQ
                     AFLRAWELRGRIRDMEAMRAWLAKVVVNESLVVVRRRKSEQRARNLYPVPDEDCRQPC
                     ERAEFRERLQDALDQLPEQTRMVVVLRLLDGMSGNEVKSLLGCSASEVSRRLHQGMER
                     LRGLLAGQYAAD"
     gene            2162..3031
                     /locus_tag="P5316_12420"
     CDS             2162..3031
                     /locus_tag="P5316_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85804.1"
                     /translation="MNCNSVRDELLQYDWDDVDRQRLMETLNHLADCDECRSAMADFD
                     RLRLTSRPAAGDAEPAGGWAAFENRLIDRMTKPCRRFGWVPPAMAAALAMAVVGWGLY
                     LGGSRGGDSAPDRSAPSANGTAVVKALTPQEIADRVEVFDQVSEVFDHRAGWVLISDR
                     RSDMGIGQAPTGKGTSLLLVRLSLLKDQSSFSSADLVIVPGHMARVSVPSQDGALVRY
                     QLMTSETDPKYLGLRVEVGSTNGQRDNGAVLATNLRLPSGKIVSAGQLVTSSGRYDVH
                     VGIYEAKASSAPL"
     gene            3223..4062
                     /locus_tag="P5316_12425"
     CDS             3223..4062
                     /locus_tag="P5316_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85805.1"
                     /translation="MVTLTPPEVRARTQMRWVYRLYQRSDGKEIDTGEAVIQVYPSPR
                     PDRLKGRLQDKRVAVWDTADGLADALTQAGLEFRRIRKAADLQLSGADVVLVGPDELR
                     DAVFDQSPLIALSEAGATVVFFEQTRPRFLAGYALNRRTARTRPTWRSGHLLMSGLRT
                     GDPESWLDGLGQEVWAIELPADEPVLEIGYWPPVVEGSQPGPIEALLAVKAVGKGRLI
                     MCQIPLGPWDTDPRTQQFLENVVSYVGTRPEPTPRPTERQVLRPAASQPVSTIAIPSG
                     ATP"
     gene            4059..5186
                     /locus_tag="P5316_12430"
     CDS             4059..5186
                     /locus_tag="P5316_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRR85806.1"
                     /translation="MRTIAVFTSVLAVTVMLLVVPPALAAESRPSVTAEATASRPAGQ
                     EDGREASRPLTVAILDFGAKDPAMPDLGPQIGEVLTAVLSGEPGFTLVDRATLARALQ
                     EHELNLSGVVSTEQAVQVGKLVGARILVTGKAFMLGKQVFITAKLIGTETSLVEGVLV
                     KDESGADVGNLVVSLATEIADRLRKAGPRLVASDDGIDPLPKLKKELAGRPKPKVVVI
                     ITEEHMRAAPLVQPVDPAVETEVKMMLRECGFDIPDVPQNELADWARAIRKEDVQSWP
                     RGLQDADLIITGEAFSEFAARIGNLVSCSARAEINMIGRKDGKIILAQRETTRAVDLS
                     ENIAGKKALEKAGRVMGVRLLEHFNKTLPARNDAAADPEKK"
     gene            5256..10772
                     /locus_tag="P5316_12435"
     CDS             5256..10772
                     /locus_tag="P5316_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85807.1"
                     /translation="MDGCLFLKVSVCALVLVLATGANTAPAASAPSLPAAAGQPAGVP
                     AEPTCAVLAEATAGLDSSPVVALLETKLSQDSRVRLVERAEITRILAEHSLSLSLAES
                     TPKSRREWGQVLRAQMLVLLQTRQVKEGKVIEAGFVETRHGLSLGSERLVWDDRAEQI
                     ASLLARRTSQVAGTLGAGVKHVFAVPPFEAEDLSPDSLQYQSAYAQLVEETLSHLPGV
                     VMADLAHAAEVARELAVSEGETSVARSLPYYLHGRHKMTGPTGNRTVSFHLELRHGQE
                     TIASIDKEAIHPDSAASFLRESCTELARKVSGGARQAPVNGATALEADLLEERCKAYR
                     LVGEWESSIRMAEAALLLDPGRHELRAMAIDSYEQMVSVWQKRRSQWEEDFRAEQNAN
                     ARKGIATRPYDINAGPVPLRDFIQCGFTAINHARHLSRTRSLAGDELWPLMRLLTIVS
                     GRYPYRSAYDWKQDPSTLKPLMRNLSDRYLDLVLHLLCSDRVGGKGAQENREQLLVDG
                     CLMACSPDWATQEEQLGIIRRIILATESLDDSFAIQYRTLELNRRVFWQPEATRQQFL
                     GELAARQEGKTRFVVECLLAMLRDQDTDQGIDIRQEVDAIARRAFPGQPIPPPQMWAV
                     YERFRSVPEFLRQGEQRRPNRVDMSAVGSKDDKIWFAAISDSDPRFCSPYPLRVNGQW
                     LALDDRRDAFVYGGLNVMTGPWQRKNLGTFEKWAPARGDFAWDGQYLWVADMRARTCL
                     QVLDGISWESVAVIGEPDGFPDMKEGCKIAPLAPGRVCVVGCTGEQLRIQSWIATIEL
                     SGDAKKGLHKSVDVFHSARQVLPARGKASTQPSGAERGFQPVFAVPVPSGRSQTPLVL
                     VGRRNWGEGPPGGAPALLVDPATRQVTILPEKWGSDWTYVVGPDRVYVLTYREVHMPL
                     VPIVAAARLPDTELREVIALDKPCWEFVAPTTAVVEGNLLHAIGKEWFTVDLSVPQLV
                     RKSPLDKKILGCRLTRSSLYGLVAYSSEGAWQVTFGEDQAPTGPAVPIGSNRLIESLS
                     DGALVSWLPNPGRYSPGSQAVDLKIGIGDVIVAVNNQKISSASDFYKISADIENKQRT
                     IEVIGQTGRRTVTAQPGKIGIFLCDYKASYGSVLTAALRACRDGRYGQARRLFEDAVA
                     AGLEYRSAALLPLIYAHCVYYSGDVAEALSILDECVSQLAAEPKRVPWVDYLAAFDPS
                     KLSPGQNTDGYLGLYLLQQTARKYPEHIPTLGELSRYQQRVLHRYPEAVSAWLDLIEK
                     ARTSGNSGLACAVFDYLLPALSTLKMGTETSAACRYLVDAEFTVSCSTGLLKPYSLAE
                     FLLDAGEPDSAIRLWLRTKAQSGKPIYPNWRHQRIIGALALDGQLDEARSLIRRFKPE
                     SDPATEAMMIGWRDLAGDYAKYARDLLARSPRDPWTHDGAFTVLSHLPRPDLAELTQI
                     HDRAVALAKAVPESDKVRFLARIRSMSLGLLMLQGDYDAALSLLSSTASRGDASYVAS
                     QREAALFLRDRAVNLTETPTPFVGTQYALRVPSGGWLLLTRDNRMGWARPDTLEISEI
                     PLPDRAWTPIHGYSFDHDHRGDQDPDISGNIMCSDSGKTVVVLGDNRTAYVLNADRRS
                     WARLIRFRVGEECEEGNDVHRSWLRRIRPVLDEFAEFMLAKAESVRDVVWFPGSSHDR
                     AVRVMLCDGTWMVHNQNDSRRFVDLSQLCARALGGPVEIYEISDPEIEGPTYLYTSHG
                     VLKWLQRKDQIEVLPFPDRTKPAAIFPLINDTETATLASLPDAGGAIYRFDLVYEKLT
                     RDAGINQCYPMTYWLQKPVEWNRQQVIDAVTKAGLPWPPPWLPIQTAESQPARR"
     gene            complement(10957..11763)
                     /locus_tag="P5316_12440"
     CDS             complement(10957..11763)
                     /locus_tag="P5316_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85808.1"
                     /translation="MCPKPGHALFMAVLCVSVMAVTAGCSQQKLAAPGAYSGATLCSD
                     SFGLTHVPCPATGFVILEKQKSRGRFPCRVAVARLVPDPKKPFSDPLSTAWALGSIKN
                     EDSTYWTSLFNTIPDIQGVGIMDPLNMEHPEANLEKIAATAAEMKTGLCVIFGPGPAE
                     SGNACVIGVIMDNARRERIACVQALATPADSYDPPPDRMIGDQRNQDVNYIAVRKFQQ
                     QVRRCIEELRSRDIPAPATQPSPWKTGRGIRSMDQQLPPIYIIPNRSMDW"
     gene            12228..13565
                     /locus_tag="P5316_12445"
     CDS             12228..13565
                     /locus_tag="P5316_12445"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRR85809.1"
                     /translation="MLWSAFDVAILSLGLYLAYHTLVWTPTGGWVPFTWWQMCLVQAP
                     AMVVAGLVFGLYEQKTLLRRSRILARSFLTAATAAALTYIIIYFLMYSIYSRRVMLLA
                     MASYLALAPTFRLMVCSAVNRYSRKFVIVGTDRKSQLSPDCQGDGLSKRYQLVGYVST
                     DALEVGRTIGKHPVLGTVDEIEQICLENQVDEVVVGRVASKNPWTVDKALRCLKIGCR
                     VTNLSTFYEDVLSEVPATLLEPNWFLFADFKHYHEAQLIMKRAMDIVLATIGLILTLP
                     FWPLVALLIKLDSPGPVFYSQARVGLLGRHFRLYKFRTMRVGSEKNGHAWAAINDPRV
                     TRVGRYLRKLRIDELPQLVNILTGTMSVVGPRPEQPEFVDELAARIRFYNERHVVKPG
                     LTGWAQINYRYGASVEDAQRKLQLDLWYIKHMCIELDLTILLRTLGTLFIGSR"
     gene            13667..15091
                     /locus_tag="P5316_12450"
     CDS             13667..15091
                     /locus_tag="P5316_12450"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR85810.1"
                     /translation="MNKSILLVNPPAEVPRECYDTPDYPAIGIASVAGYLIKNGVECS
                     VIDGKLSRLSVDDTIQEIIGRRPRILGLTAMTHMIVTAHRIASAVKAQCPDTIIVLGG
                     FHASFLPERTLREFPSFDYIVVGEGEIAFHKFVRAVFDGGDPAEIQGIAGRRGGAIRV
                     NGRGEIADNLDDLGMPAWELFPRARMYPVMSQRGCPFGCNFCSRPYGRKVRRRSVPHV
                     IAEIKRNIEQFGCTVTDFYDETFTVNKKHAAELCRAIIAEGINRKVRFWAYVHANTID
                     LPTARLLKEANFSSVGFGVESGNPQIMARMKKNVGPEDVLRAARVLREAGLDFAAYFI
                     IGHPNETRETAWDTIRLAAKVNPNSVAIGLMVPYPGSEIWELATHGQGGYKLIGVNWE
                     DFNKQIGNALELENLPRRTMERLQLQGYLYVYLRNLRFREMFEAIWINRKRIAFILRK
                     LIRPSRRVASSSWLEPSRQTLPAT"
     gene            15218..15907
                     /locus_tag="P5316_12455"
     CDS             15218..15907
                     /locus_tag="P5316_12455"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRR85811.1"
                     /translation="MSARTGGHHGSVGKQVVLPWGQSIRICAAGIRTRPGRSLLTLAG
                     VVLAVAFMAGVMTSARLADAIVRTGDTNTVFALARSTGIDLGSETARLNQQRRNTWLA
                     GLSILVATVGIANSVLMSVTERFREIGTMKCLGAIDSFVVRLFFLESAFAGMIGSAVG
                     AVLGILLATVRIAVAVGPAAIPLGTLAIDALQILAAALVVGTLITILAAIYPAYVAAR
                     MQPVEALRVEE"
     gene            15910..>16366
                     /locus_tag="P5316_12460"
     CDS             15910..>16366
                     /locus_tag="P5316_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR85812.1"
                     /translation="MPSDRADSIIRTRDVRKVYVRGCQRVEALRGVTIDIAAGEYISI
                     MGPSGSGKSTFFNMVGGLDKPTSGQVYIDEVDIAQLDVYELAWLRCRKIGYIFQTFNL
                     IPVMTALENVTLPMIFAGLNSDEAREKGAELLRIIGLGDRLEHRPFELSG"
BASE COUNT         3084 a         4738 c         5271 g         3273 t
ORIGIN      
        1 cggcgatacc tttgcatcat tgggattcgc ggaatgccgg cagacacaag gcgcgctgta
       61 atgcgatgcg ccggccgctt gcggggtgct cgacctgcgg cggaccctga tcggcgcaca
      121 gcgtttacct tgattgagat cctggtggtg gtggcaatcg tcgttttgct cacggccatt
      181 cttattccgg ctttgtcaca agcccgggcc caggcgcggc gctcggtctg tctttcgaac
      241 atgtcgcact tggccaaggc cgtcctctcc ttttccgcca cgcacaagga tcgcggccag
      301 ctttacgcgg actgggatta tgtggatgcc gtggatcctg accacagcat ttacgcatat
      361 cagaatgggt ggtttgagga cggcgtactg aagaaccccc acaagccgta tctcaagccc
      421 tggcctgtgg cgtacgcagg cgaattgggg atgccttcgg tccggaatac ccaccagtac
      481 ttcctggacg aaacgaccta tctgaagggg tatccggata ccgagcagcc ggaatatcac
      541 ttcaggcgat tcggcaaaca cgagcccctt tattgtcctg cagacaagtt ctcgattcgg
      601 gagacgaatt cgccggagca cgtttatgga gtactctctt atgccatcaa tgaggatgtc
      661 ttcggggcaa ggcagggcca gtgttgcacc cgctcgggct ggaaggacca ggcccttggg
      721 atcatgagcc ggatcgccaa ttcgtccgag gttgcgatgt tcaccgatgc cgggcacgag
      781 acacttgaaa ggagccaggc tctcaagagt gacggcagca tcaggatccg ggccgggcag
      841 tgggtaccga catggttccg gtcggcccag ggcgcggcct ttctcaacga ggtgaacgca
      901 aaggtcatcg tggcggttcc gatcgagcgg catggcaaga acggcggtat cagcgtcgcc
      961 tacgcagacg ggcacggttc gtttgttgcc gggtcggggg cctgggaact atcgaccgga
     1021 ttccccgcct aatccacgga gcagctcgta cgggtccctc tccggtacgt tccccgagcc
     1081 ccgcgtgtca atccctacgc cccgtgatcg gcctagaggc acggagcacg gcgaagagcc
     1141 gttggctcgg agagtgtgcc cgcaagaagc gggttacgat ggctatcttt ccgggcttcc
     1201 gtcaggcttg ctggtgcttc ggcaactgtt ggcatgcgtt catcgctgct caggatttca
     1261 tcccatgccg gggttcgaag cttacggcca gacatttggg tggcttgcgc gcgtgtttcc
     1321 ggaccgcggt gcgcttgggt tggggtccgg aagaaggcgg ttcgccttgg gtgcgccgta
     1381 attgtttgtc gcagatggtt ttatgggtga tcaattggtt gggcctggct ttggccacaa
     1441 aactgtgccg gttgtccgaa atcgcggtat cctggcgcat cctaataggc agaacagcga
     1501 cggaccggaa gggtgaaaca ttgacagtgt tcgcggattt cacgaaggac gccgaggcag
     1561 cacttattgc tcaggtcaaa agcaatagtc gcctgttttt ccgcttggcc tacggggttg
     1621 tgcgtgatgt tcaagctgcc gaggatgtct gccagcaggc ttttctacgg gcctgggagc
     1681 tccgcggccg gatccgcgat atggaggcca tgcgggcctg gctggccaag gtggtggtga
     1741 atgagagtct ggtcgtggtt cgacgacgca agagtgaaca acgtgccagg aacctgtatc
     1801 cggtgcccga cgaggattgt cgacaaccgt gcgagcgagc cgagttccgg gaacgactgc
     1861 aggatgcgct tgatcaattg ccggagcaga cgcgaatggt tgtcgtgttg cggctgttgg
     1921 acggcatgag cggcaacgaa gtgaaatcac tgctgggttg cagcgcctcc gaggtctcca
     1981 ggcggcttca tcaaggaatg gaacgtcttc ggggtttgtt ggccggtcag tacgccgcag
     2041 attgaggaat cagccggcct ccaacggtct tcgtgccgac gttgcttctg ccgtcgggaa
     2101 gcacggcagc cagacacgac aaatcagatg cgacgtgatg atggaaaagg atgactcaac
     2161 gatgaactgt aactcggtca gagatgagct actgcaatac gactgggatg atgttgaccg
     2221 ccagaggttg atggaaacat tgaatcatct ggccgattgc gacgagtgtc ggtcggccat
     2281 ggccgatttc gaccgcttgc ggttgaccag ccggccggcg gcaggggacg ccgagccagc
     2341 gggcggttgg gcggcatttg aaaacagact gatcgatcga atgaccaagc catgccggcg
     2401 gtttgggtgg gtgccgccgg cgatggccgc cgctctggcc atggccgttg tgggttgggg
     2461 cttgtacctc ggagggagta gagggggtga cagtgcgcct gatcggtctg cgccttcagc
     2521 caatggaacg gcagtcgtca aagccctcac gcctcaggaa atcgccgacc gtgtcgaggt
     2581 cttcgaccag gtttcggagg tttttgacca ccgtgcgggc tgggtgctga tttcggatcg
     2641 gcgatcggac atgggcatcg ggcaggcccc gactggcaaa gggacatccc tgctgctggt
     2701 aaggctgagt ctgctgaagg accagtcgtc tttctccagc gccgacctgg tcattgttcc
     2761 gggtcacatg gcccgagttt ccgtgccttc gcaggatggt gcgcttgtgc gttaccaact
     2821 gatgacatcg gagacggatc cgaagtatct cggcctgcgt gtcgaggtcg gttccaccaa
     2881 tgggcaacgt gacaacggag ccgtgctggc gacaaacctg cggctgccat cgggcaagat
     2941 cgtttctgct gggcaactgg ttacttcttc gggacgttat gacgtgcacg tgggtatcta
     3001 tgaggcaaag gcatcgtcgg cgccgctatg atcgttgcga gaataatgat gttgattctg
     3061 tcaaacgagg gccattggta cgggggccag gccggtacga tatccttgca ttgggtcgcc
     3121 cggttcgaac agcccgaggc tgtgcttgtc tgggacttgt tgatcgggga agttcgtgtg
     3181 gccggcgatc gactggccgt ccaaagggga agcagaccta gtgtggtgac gctcacaccg
     3241 ccggaagtgc gggccaggac gcaaatgcgc tgggtgtatc gcctttatca gcgaagtgac
     3301 ggcaaggaga ttgacacggg cgaagcggtc attcaagtct atccgtcacc cagaccggac
     3361 cgcctgaaag gtcgattgca ggacaagcgg gtcgcggtct gggacacggc ggacggcttg
     3421 gccgacgcct tgacccaagc cggcttggag ttcagacgaa tccggaaagc ggcagacctt
     3481 caattatccg gggcggatgt ggttctggtt gggccggacg agttgcgcga cgcggtgttt
     3541 gaccagtcgc cgctgattgc cctttcggaa gcgggagcca cggtcgtgtt cttcgagcaa
     3601 acgcgcccgc ggtttcttgc cggctacgcg ttgaaccgcc ggacggcacg tacccgaccc
     3661 acttggcgaa gcggccacct gctgatgagc ggcttgagga ccggcgaccc ggaatcctgg
     3721 ctggatggat tggggcaaga ggtctgggcg attgagctgc cggctgacga gccggttctc
     3781 gaaatcggct actggccgcc ggtcgtcgag ggttcgcagc cgggccccat cgaggctctg
     3841 ctggcggtga aggcggttgg gaagggcaga cttatcatgt gccagatacc gttggggccg
     3901 tgggacacgg accctcgcac gcaacagttc ctggagaatg tggtcagtta cgtcgggacg
     3961 cgaccggagc cgacgcctcg gccgaccgaa cgtcaggtat tgcgtccggc tgcctcgcag
     4021 ccggtgtcca ctattgctat accctctgga gctacgccat gagaacaata gccgttttca
     4081 caagcgtgtt ggctgtgact gtgatgttgc ttgtcgtgcc gcccgccctg gccgcggagt
     4141 cgcgcccctc ggtgacggcg gaggcgacgg cctcgcggcc tgcgggccag gaggatgggc
     4201 gtgaagcttc tcgcccgctg accgttgcga ttctcgactt tggcgcaaag gaccctgcca
     4261 tgcccgacct agggccgcag atcggcgagg ttctgacggc cgtgctttcg ggtgagccgg
     4321 gcttcaccct ggtggaccgg gccacgctgg ctcgggcgct gcaggagcac gaattgaacc
     4381 tgtcgggggt ggtcagcacg gagcaggccg tccaggtggg caagctggtc ggggcgcgga
     4441 ttctggtgac cggcaaggcg ttcatgctcg gcaagcaggt gttcatcacc gccaagctga
     4501 tcgggaccga gacgtccctg gtcgaggggg tactggtcaa agatgagagt ggcgcggacg
     4561 tgggcaatct ggtcgtttcg ctggccaccg agattgccga ccgcttgcga aaggccggcc
     4621 ccaggctggt ggccagtgat gatggaatcg atccgctccc caagctgaag aaggagctgg
     4681 ctggtcgtcc caagccgaag gttgtggtga tcatcactga ggaacacatg agggccgccc
     4741 cactcgtgca gcccgtggac ccggccgtcg agaccgaggt caagatgatg ctccgcgagt
     4801 gcggcttcga cattccggac gtgccccaga acgaactggc cgactgggcc agggccattc
     4861 gcaaggagga tgtgcagtcg tggcctcgcg gcctgcaaga cgccgacctg atcatcaccg
     4921 gcgaggcctt cagcgagttc gccgcccgaa tcggcaacct ggtgagttgc tcggcccgcg
     4981 ccgagatcaa catgatcggc cgcaaggacg gtaagatcat cctcgcccag cgcgaaacca
     5041 cccgcgccgt cgacctctcg gagaacatcg ccggcaagaa ggccctggaa aaggccggcc
     5101 gggtaatggg cgtccggctg ctggagcact tcaacaagac gctgccggct cgcaacgacg
     5161 cagccgcgga tccggaaaag aagtgacggc gcatcggtag tcctgcgtgt gcgctgaaca
     5221 cgatcaggcg cgtgtcacgg gaggagaatg atttgttgga cggatgcctc ttcctcaaag
     5281 tgtctgtgtg cgcacttgtc cttgttctcg ccactggggc gaataccgca ccggcggcat
     5341 cggcgccgag cttgcccgcg gcagcgggtc aaccggccgg agtgccagcc gagcccacct
     5401 gtgcggttct ggccgaggcg acggccgggc tggactcgtc gcctgtggtg gctctgctgg
     5461 agacgaagct gtcgcaggat tcgcgggtcc ggctggtgga acgggcggag atcacccgga
     5521 ttctagctga gcactcgctg tctctgagcc tggccgagag tacgccgaag tcgcggcggg
     5581 aatggggaca ggtgctgcgg gctcaaatgc tggtgctgct gcaaacgcga caggtcaagg
     5641 aaggcaaggt cattgaggct ggcttcgtgg agacgcgcca cgggctgagc ctgggaagcg
     5701 agcggctggt ctgggacgac cgggccgaac agattgcctc cctgctggcc cggcgcacca
     5761 gccaggtggc tgggaccctg ggggcgggcg tcaaacacgt ctttgccgtg ccgccgttcg
     5821 aggccgagga tctttcgccg gattcgctcc agtatcagag cgcctatgca caactggttg
     5881 aggaaactct ttcacacctg ccgggcgtgg tgatggccga tctggcccac gccgcagaag
     5941 tggcccgtga actggcggtg agcgaagggg agacatcggt cgccaggtct ctgccctatt
     6001 accttcacgg ccggcacaag atgaccggcc cgaccgggaa tcggaccgtc agtttccacc
     6061 tggagcttcg tcacggccag gagaccattg cctcgataga caaggaggcc atccatccag
     6121 actccgccgc atcgtttctg cgtgaatcct gcaccgaact ggcccgcaag gtgagcggcg
     6181 gcgcgcggca ggccccggtc aatggcgcaa ctgccctgga ggccgatctg ctggaggagc
     6241 ggtgcaaggc ctatcgactc gtcggcgagt gggaatcgtc gattcgcatg gccgaggcgg
     6301 cgctgttgct ggacccgggg cggcatgaac tgcgggcaat ggccatcgat tcgtacgaac
     6361 agatggtgtc tgtgtggcag aaacgacgct ctcaatggga ggaagacttt cgtgcggagc
     6421 agaacgccaa cgcccgaaaa ggcatcgcca ctcgacccta tgatattaac gcagggccgg
     6481 ttccgctgag ggacttcatc caatgcggct tcacagcaat caatcatgcc aggcacctgt
     6541 cgcgtacccg ttcgctcgcc ggcgatgagc tctggccgct catgcggctt cttactatag
     6601 tgtcaggacg atatccttac cgctccgcct acgactggaa acaagacccc tcaactctta
     6661 agcctctcat gcggaaccta tcggatcggt atctggattt ggtgcttcat ctgctttgta
     6721 gtgatcgtgt cggtgggaag ggagcacagg aaaaccgcga gcagctcctc gtcgatgggt
     6781 gtcttatggc gtgcagtccc gattgggcga cgcaggaaga acaactgggg atcattcgca
     6841 gaatcatcct ggcgacagaa tcgctcgatg attcatttgc cattcagtac agaacgcttg
     6901 agctgaaccg ccgcgtgttt tggcagccag aggcaacacg ccaacaattc ctgggggagc
     6961 ttgcagcacg acaggaagga aagacacgtt ttgtggtcga gtgcctgctg gcgatgctga
     7021 gagatcagga caccgaccaa ggcattgata ttcggcagga agtcgatgcc attgcacgga
     7081 gggcttttcc tggacagcca attcctcccc cacagatgtg ggcggtctac gagcgttttc
     7141 gcagtgttcc tgaattcttg cgacaaggag aacaacgccg gccaaaccgc gtcgatatga
     7201 gtgctgtcgg ctccaaggac gacaagatct ggtttgcggc gatttcagat tccgacccac
     7261 gattttgctc gccatacccg cttcgtgtaa acggtcaatg gctggccttg gatgaccggc
     7321 gtgatgcgtt cgtttatggc ggcctgaatg tcatgaccgg accatggcaa aggaaaaacc
     7381 ttggcacgtt cgagaagtgg gcaccggcgc gaggtgactt cgcatgggat gggcagtatc
     7441 tctgggtagc tgacatgaga gcacgtacct gtctccaggt gctcgatggt atctcatggg
     7501 agtctgtggc ggtcattggc gagccggacg gttttcctga catgaaggag ggctgcaaga
     7561 tcgccccact tgccccaggg cgggtctgcg tggtgggatg cacaggtgag caactgcgaa
     7621 ttcagtcctg gattgccaca attgaactta gcggggacgc gaagaaaggc ctgcacaaga
     7681 gcgtcgacgt cttccacagt gctcggcagg tactacccgc tcggggcaag gcgtcgactc
     7741 aacccagtgg cgccgaacgt ggctttcagc ccgtcttcgc ggttccagtt ccgtcgggca
     7801 ggagccaaac gccgctggtc ttggtgggcc ggcgcaactg gggtgaaggg ccgcccgggg
     7861 gagcgccggc cttgcttgtg gacccggcca cgcggcaggt cacgatcttg ccagaaaaat
     7921 ggggcagtga ctggacctac gtcgtcggtc cggaccgggt ctacgtctta acctatcgag
     7981 aagtacacat gccgttggtg ccgatcgtag ccgcggcccg cctcccggac actgagcttc
     8041 gagaagtgat cgcgctcgac aagccctgct gggagtttgt cgcccccacg accgccgtgg
     8101 tcgaagggaa tttgctgcac gcaatcggaa aagagtggtt caccgtggac ctgagcgtgc
     8161 cgcaactggt caggaaaagc ccgctcgata agaagatact tgggtgcaga ttgacgcgat
     8221 catcgctcta tgggttggtc gcctacagtt ctgaaggcgc atggcaggtt actttcggcg
     8281 aggatcaggc ccccaccggc ccagctgtgc ccatcgggtc gaacagactg attgagagcc
     8341 tgtcagatgg tgctcttgta tcatggctgc ccaatcccgg gagatacagt cccggttccc
     8401 aggccgttga tctgaagatc ggtatcggtg atgtcatcgt cgcggtaaac aatcagaaga
     8461 tttcgtcggc atctgatttc tacaagatat ccgcggatat tgagaacaag cagcggacta
     8521 ttgaggtgat cggtcagacc ggccggcgca ctgtgacggc gcagcccggc aagatcggca
     8581 tattcctctg cgactacaaa gcgtcatatg gttctgtgct aacggcggca cttcgtgcat
     8641 gtcgggatgg gcggtatggc caggcccgga ggctgttcga ggacgccgtg gcagccggcc
     8701 ttgagtaccg gtcggctgct ctgctcccgt tgatctatgc ccattgcgtc tactactccg
     8761 gtgacgtcgc agaggctctg tctattctcg atgaatgcgt cagccaactc gcagcggagc
     8821 ccaaacgcgt tccgtgggtg gactatctgg ccgcgtttga tccgagcaag ctttcgcctg
     8881 gtcagaacac cgatgggtac ctcggtctat acctgctgca gcagaccgcc cgtaagtatc
     8941 cagagcacat acccactctt ggagagttgt ctcgctacca gcagcgagtt cttcaccgct
     9001 atccggaggc ggtctcagct tggctggatc tgatcgagaa agccaggacg tcgggcaaca
     9061 gtggcctcgc gtgcgctgtg tttgactacc tgctgccggc tctcagcacg ctcaagatgg
     9121 ggaccgaaac ctctgcggcc tgccggtacc tggtggatgc cgagttcacc gtttcctgtt
     9181 ccacaggctt gctcaagcct tactcgctgg cggagttcct gctcgacgcc ggcgagccgg
     9241 attctgcaat ccggctgtgg ctacgtacca aggcgcaatc cgggaagccc atctacccta
     9301 actggcgaca tcaacgcatc atcggcgctt tggccctgga tggtcaactt gacgaggccc
     9361 ggtctctgat caggcgtttt aagcctgaat ctgacccggc taccgaggcg atgatgatcg
     9421 gctggcggga tctggccggg gattatgcta agtatgcgag agatctgctt gcccgatcac
     9481 cgcgggaccc gtggactcac gacggggcat tcaccgtcct gagccatctg ccccggccgg
     9541 acctcgccga actgacacag atccacgacc gggcggtggc attggccaaa gcggtgcccg
     9601 aaagcgacaa ggtccggttc ctcgcaagga tacgtagcat gagcctcggt ctgttgatgc
     9661 ttcagggtga ctacgatgcc gctctttcgc tgctaagcag caccgcatcg cgaggagatg
     9721 cgtcctatgt ggccagtcaa cgtgaggccg cgcttttcct gagagaccgc gcggtgaacc
     9781 taacggagac tccgaccccc tttgtcggca cgcagtacgc tctgcgagtg ccatccggcg
     9841 gctggcttct gttgacccgt gacaatcgaa tgggctgggc aagacccgac actctggaga
     9901 tcagcgaaat cccgcttcct gatcgcgcat ggactcccat acacggatac agctttgatc
     9961 acgatcaccg tggtgaccag gacccggaca tctcgggtaa tatcatgtgt tcggattcgg
    10021 gcaagacggt cgttgtcctg ggagataaca ggacggcata cgttctgaat gccgaccggc
    10081 gaagctgggc acggctgatc cgcttccggg tcggcgagga atgcgaggag ggcaatgacg
    10141 tgcacagatc gtggctcaga aggattcggc cggtgctgga cgaattcgcc gagttcatgc
    10201 tggccaaggc cgaatccgtc cgagacgtcg tatggtttcc cggcagcagc catgatcgtg
    10261 cggtaagagt catgctgtgc gatggaacgt ggatggtgca caaccagaat gacagtcgga
    10321 gattcgttga tctaagccag ctctgcgcca gggccctggg tggtcctgtc gaaatctatg
    10381 agatctctga tcctgagatc gaggggccaa cctacttgta taccagccac ggcgtgctca
    10441 agtggcttca acgcaaggac cagatcgagg tactcccctt cccggaccgg accaaaccag
    10501 cggcgatctt cccgctgatt aacgacacag agactgctac tcttgcgtcg ctgcccgatg
    10561 cgggcggagc catttaccgg ttcgacttgg tttacgagaa gctcacccgc gatgcgggca
    10621 tcaaccagtg ctatccgatg acctactggc tgcaaaagcc ggtcgagtgg aaccgccaac
    10681 aggtgatcga cgcggtgacc aaggccggtc tgccgtggcc cccgccgtgg ctgccgatcc
    10741 agacggccga atcgcaaccg gcccggcggt aagaagaggg gggcggttcc acgatctgat
    10801 atacccgcag acgccgttgc cgacctgccg atatgtcagc agaaagcgga aaccccaaca
    10861 gccgaacggt gcgatagccc ggcagtcgaa ccgtctgacg gccttgcagt ggaacaatcc
    10921 aatagccgaa tagtctggtt gccgaaaggc ctcagcctac cagtccatcg accggttggg
    10981 gatgatgtag atgggcggga gttgctggtc catcgagcgg atgccgcggc cggtcttcca
    11041 ggggcttggc tgggtggccg gggcggggat gtcgcgggat cgcaactcct cgatgcaccg
    11101 gcggacttgc tgctgaaact tgcggacggc gatgtagttc acgtcctggt tccgctggtc
    11161 gccgatcatc cggtcaggcg gaggatcata gctgtccgcg ggagtggcca aggcctggac
    11221 gcaggcaatc ctctcgcggc gggcgttgtc catgatcacg ccgatgacgc aggcatttcc
    11281 cgactccgcc gggcccggtc cgaagatgac acacagaccg gtcttcatct ccgcggccgt
    11341 tgcagcgatc ttctccaggt tagcctcagg gtgttccatg ttcagcgggt ccatgatgcc
    11401 gacgccctgg atatccggta tggtgttgaa caagctggtc cagtaggtgg aatcctcgtt
    11461 cttgatcgac ccgagtgccc acgcagtgga caggggatct gagaatggct tcttcggatc
    11521 aggaacgagc ctggccacgg ccacccggca tgggaatcgc cccctgctct tctgcttttc
    11581 caggataacg aagccggtgg ccggacacgg gacgtgtgtg aggccgaagc tgtcactgca
    11641 gagggtcgct cccgagtaag ccccgggtgc ggcgagtttc tgctgactgc aaccagcggt
    11701 gacggccata acagatacac acaggacggc catgaacaac gcgtgtccgg gcttggggca
    11761 catgccactc gctctccatg cgtacgggaa gtcggtccaa taaagtccct atagttttat
    11821 cggcatctgt ggggcccact gaaggaaacg ccgacggttt tcgggccttc tgaaggccca
    11881 cgccctcagc ggagaaaggt ccgatattca ggcggaccga tgcatggcag gggagtcgtt
    11941 cgcggggcac ttgtcttttc tctgctcggt ggtgaatcgc gtaaccgccc gcgacggtag
    12001 ctcatcgcct ccctcgatcg cgccgataac aacaacgccc aagaaaccgg gcaggggcga
    12061 cgtgtatgga ctccccgacg caaagcgttt ttattaaacc tggttcgctg ccaggcactc
    12121 tcccgttgcg ggctcatctt cggctggtca aacccgaatc agcggcaccg gccgttggtc
    12181 gcacctggtt tggtacgttc ggcaatacgc tccttgagat gccctggatg ctctggagcg
    12241 cctttgatgt ggcgattctg tcgcttggtt tgtatctggc ctatcacacg ctggtttgga
    12301 cgccgacggg cggctgggtt cccttcacct ggtggcagat gtgtctggtt caggcgcctg
    12361 ccatggtcgt tgcggggctg gtcttcgggc tttacgagca gaagacgctg cttcgccgat
    12421 cgcgtattct ggcccgttct tttctgacgg cggcgacggc ggcagctctg acgtatatca
    12481 tcatctactt cctgatgtac tcgatctaca gtcgccgggt gatgctcctg gcgatggcca
    12541 gctaccttgc cctggccccg actttccgcc tgatggtttg ctccgctgtc aaccgttaca
    12601 gtcgcaaatt cgtgattgtc ggcacggatc gcaagtcgca gttgtcgccg gattgccaag
    12661 gcgacggcct gtcaaaacga taccaactgg tcggatacgt cagcactgac gctctggagg
    12721 tcggccgcac gatcggcaag cacccggtgc tgggcacggt ggacgaaatc gagcaaatct
    12781 gcctggagaa tcaggtggat gaggtggtcg tcgggcgggt cgcgtcaaag aatccgtgga
    12841 ccgtggacaa ggccctgcgt tgcctcaaga tcggctgccg ggtaacgaat ctctcgacct
    12901 tctacgagga cgtgctcagc gaggtgcccg caacactgct cgagccgaac tggttccttt
    12961 ttgccgactt caagcactac catgaggccc agctcatcat gaagcgggcc atggacatcg
    13021 tcctggcgac catcgggctg attctgacac tgccgttctg gccgttggtt gcgctgttga
    13081 tcaagctcga cagtcccgga ccggtcttct acagccaagc gcgcgtgggc ctgctggggc
    13141 gccactttcg gctttacaag ttccggacca tgcgggttgg ttcagagaag aacggccacg
    13201 cctgggcggc gatcaatgat ccgcgcgtga cccgtgtggg gcgctacttg cgaaaactcc
    13261 gcatcgacga gctgccgcag ttggtcaaca tcctgacggg gacgatgtcc gtcgtgggtc
    13321 cgcgccccga gcagccagag tttgtcgatg aactggcggc caggattcgc ttttacaacg
    13381 agcgccacgt ggtcaaacca gggctgaccg gatgggccca gatcaactat cgctacggtg
    13441 ccagcgtcga ggacgcacag cgcaagcttc agcttgatct ctggtacatc aagcacatgt
    13501 gcatcgagct ggacctgacg atcctgctcc gcacgctggg aaccctgttc atcggttccc
    13561 ggtagagtcc tcgccggcgg atcacggttg accgaaccct gaacccttct tcgccaactt
    13621 cacccttgcc ggcgggcatc cgataacatg ttgagaggac ccaggcatga acaaaagcat
    13681 tcttctggtc aacccgccgg cggaggttcc ccgcgagtgc tacgacacgc cggattatcc
    13741 ggcgatcggc atcgcgtcgg tggcgggtta cctgatcaag aacggagtgg aatgctccgt
    13801 catcgacggg aagctgagcc gactcagcgt ggacgacacg attcaggaga ttatcgggcg
    13861 tcggccgcga atccttggtc tgacggcgat gactcacatg atcgtcaccg cccacaggat
    13921 tgcctctgcg gtcaaggccc aatgtcccga taccatcatc gtccttggcg gcttccacgc
    13981 cagttttctg cctgagcgaa cccttcggga gtttccgagc ttcgactaca ttgtggtcgg
    14041 cgagggcgag attgcattcc acaagttcgt gcgggcggtc ttcgacggcg gtgatcccgc
    14101 cgagatccag ggaatcgccg gccgtcgtgg aggagcgatt cgagtcaacg gccggggcga
    14161 gattgcggat aaccttgatg acctcggcat gccggcctgg gaactgtttc cgcgtgcgcg
    14221 catgtacccg gtgatgagcc agcgcggttg cccgttcgga tgcaacttct gcagccgacc
    14281 atatggtcgc aaggtccgcc gccggagcgt gccccacgtc attgccgaga tcaaacgcaa
    14341 catcgaacag ttcggttgca ctgtcaccga cttctatgat gagactttca cggtcaacaa
    14401 gaagcacgcc gccgagctgt gcagggccat cattgccgag ggaatcaacc gcaaggtccg
    14461 tttctgggct tacgtgcacg ccaacaccat cgacctgccg acggccaggc tgctcaagga
    14521 ggccaacttc agcagcgttg gtttcggggt ggaatccggc aatccgcaga tcatggcgcg
    14581 catgaaaaag aacgtcgggc cggaggatgt gctgcgcgcc gcccgcgtgc tgcgagaggc
    14641 cgggctcgac tttgccgcgt atttcatcat cggtcatccc aacgagaccc gcgaaacagc
    14701 ctgggacacc atccgtctgg ccgcgaaggt caaccccaac tcggtggcca tcggtctgat
    14761 ggtgccgtac cccggctcgg agatctggga gctggctact cacggccagg gcggctacaa
    14821 gctgatcggt gtcaactggg aagacttcaa caagcagatc ggcaacgccc tggaattgga
    14881 gaacctcccc cgccggacca tggagcgcct ccagctccag ggatatctct acgtctacct
    14941 gcgcaacctg cgcttccgtg agatgttcga ggccatctgg atcaaccgca aacgcatcgc
    15001 cttcattctc cgcaagctca tccgcccctc ccgccgcgtg gccagtagct cgtggctgga
    15061 gccctcgcgg caaacgttac ccgcaacttg acggcgccaa tgcccaggct tgcgataacg
    15121 agagggggcc caagcaaatg cttttgtcca ccggggactg tttctctaca ataccgctat
    15181 ccgtgtcctg cattgatggt gcctggagat tctcgacgtg tccgcaagaa ccggcggtca
    15241 tcacgggtcg gttggcaagc aggtggtgct gccgtggggg cagtcgattc gcatctgcgc
    15301 ggcggggatc cgcacgcggc cggggcggtc gctactgacg ctggccgggg tagtgctggc
    15361 ggtggctttc atggccgggg tgatgacctc ggcgcggctg gccgacgcga tcgtccggac
    15421 cggcgacacc aacaccgttt tcgccctggc ccgaagtacg ggcatcgacc tgggcagcga
    15481 aaccgcgcgg ctcaaccagc agcgccgcaa cacctggctt gccggactgt cgattctggt
    15541 ggccaccgtt ggcatcgcca attccgttct gatgagcgtc accgagcgat tccgtgagat
    15601 cgggaccatg aaatgtctcg gagcgatcga ctcgttcgtg gtacgtctgt tttttctgga
    15661 gagtgccttc gcggggatga tcggttctgc cgtcggggcc gtgctgggca ttctcctggc
    15721 gacggttcgc atcgcggtgg cggtgggccc ggcggcgatt ccgctgggga cgttggcgat
    15781 tgacgctttg cagatccttg ccgccgcgtt ggtcgtcggt acgctgatca ccattctggc
    15841 cgcgatctac ccggcttacg tcgccgcccg gatgcagccg gtggaggcct tgagggtcga
    15901 ggaatgagca tgccatctga ccgggccgac agcatcatcc gcacccgcga cgtgagaaag
    15961 gtctacgtca gggggtgcca gcgggtcgag gcgttgcgag gcgtgaccat cgacatcgcc
    16021 gcaggcgagt acatttcgat catgggcccc agcggttcgg gcaagagcac cttcttcaac
    16081 atggtcggcg gcctcgacaa gcccaccagc ggccaggtct acatcgatga ggtggatatc
    16141 gcccagctcg acgtatacga gttagcctgg ctgcgctgcc gcaagatcgg ttatattttc
    16201 cagaccttca accttattcc ggtgatgact gccttggaaa acgtcacctt gccgatgatc
    16261 ttcgcggggt tgaacagcga tgaagctcgc gagaaaggcg cggagttact caggatcatc
    16321 ggcttgggcg atcgcctcga acaccgtccg tttgagctct cggggg
//
LOCUS       DAOTPW010000127        16332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3542_length_16332_cov_6.227499, whole genome shotgun sequence.
ACCESSION   DAOTPW010000127 DAOTPW010000000
VERSION     DAOTPW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16332
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3542_length_16332_cov_6.227499"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="P5316_12465"
     CDS             complement(<1..899)
                     /locus_tag="P5316_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85813.1"
                     /translation="MSGILAFSPNALCFLACLVMEPATGYKRCMISKELAEKLVRTSR
                     PLRGLHGSRMVLLGIGLLLGAALARPEYLTQSGIPLWVWPHVVLLFIVAVVLLKLVRR
                     QRQATLTLAAFESLQLQEWEQARRHLVKLLARPVNHAPARTEALLGLGNLAEIGHDYD
                     AAQEVYEAILNEAVANPVQLLMTRMALAATLLRTGQTADAVEMVDRLDRTSLPDSLKA
                     HVELVSLFREVVMGQAADGLVKADYRRALFREHLGTRAGYGYGLLAAAYDQANLPSPA
                     QKCWADATLLVRADALVDRFGELA"
     gene            1014..2516
                     /locus_tag="P5316_12470"
     CDS             1014..2516
                     /locus_tag="P5316_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR85814.1"
                     /translation="MRMLILVAVVAGTFVPAVSDAENTPRLPNFVIIFIDDMGYADIG
                     PFGAKDYPTLNLDRMAQEGRRFTDFYVAQPVCSASRASLMTGCYNVRVGILGALGPRA
                     DHGINADEMTLAEVCKQKGYATACYGKWHLGHHPKFLPTNHGFDDYFGLPYSNDMWPY
                     HPEITHLPMEERYKRWPHVPLIEGTKIINPQITDEDQNMLTTWYTERAVKFIDRNKDR
                     PFLLYVPHSMVHVPLHVSEKFAGKSKRGLFGDVVMEVDWSVGEILGAIRRHGIEKNTM
                     VIFTSDNGPWLSYGTHAGSAKPLREGKGTTFDGGCREPTVMWWPGTIPAGTVCITPAM
                     TIDILPTIAHLIGAKLPNHKIDGKNIWPLIEGRPDARSPHEAYFFYWGEALEAVRMGR
                     WKLHFSHPYRTLGNRKGGTDGKPEPYEQAEIGLTLFDLELDPGETTDVKDQHPEVVAA
                     IEKLADRMREDSGDSARGITGTGRRNPGRLEKGDERFVVKDGKQLLARPQ"
     gene            2716..3132
                     /gene="mscL"
                     /locus_tag="P5316_12475"
     CDS             2716..3132
                     /gene="mscL"
                     /locus_tag="P5316_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRR85815.1"
                     /translation="MDPTKKALSLLEEFKNFALKGNVIDLAVAVIIGGAFGKIVDSLV
                     KHLIMPLISVIMPGEQSYLGWHWVIRGKEVPYGLFIGEVVNFLIVALALFIFIVKFLG
                     WIMKFKKQEAAAPAAPPSLTKDQELLMEIRDLLKKA"
     gene            3187..4593
                     /locus_tag="P5316_12480"
     CDS             3187..4593
                     /locus_tag="P5316_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR85816.1"
                     /translation="MVAAQRPNVVFILVDDLGYMDIGANNPNTFYETPNINRLASTGM
                     RFTNAYAACPVCSPTRASILTGKYPVRTGITDYINPAGGNQPQGWKRNTRLLPAPYSD
                     RLALEEVTLAEELKEAGYATFFAGKWHLGPEGFYPEDQGFEINKGGCEWGNPQGGNKY
                     FSPYGNPRLPDGPPGEHLPHRLATETVRFIETHRDRPFLAYLSFYSVHTPLMAREDLT
                     HKYLQKKERIKRTGPDFGTEGERQVRLVQDHAVYAAMVEAMDLAVGKVLDALERLHLD
                     DKTAVFFMSDNGGLSTSEGSPTSNLPLRAGKGWLYEGGIREPMLIRWPGVAKPGSVCD
                     TPVTSTDFYPTILEIAGLPLRPHRHLDGVSLVPLLKGGTLQRGPLFWHYPHYGNQGGS
                     PGGAIRDGDWKLIEWFESGRLELYNLREDIGEKNNLAAAKPDRVASLHEQLREWQKRL
                     EAKLPVPNPAYAEPKTPR"
     gene            complement(4594..4803)
                     /locus_tag="P5316_12485"
     CDS             complement(4594..4803)
                     /locus_tag="P5316_12485"
                     /inference="COORDINATES: protein motif:HMM:NF025436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HRR85817.1"
                     /translation="MSMIGDIAGLSTAMSTARLQTAVGAKVLKMAMQQNGQVVSKLLE
                     SAMENMQAIVTQFAEDLGNNIDVRA"
     gene            5184..6767
                     /locus_tag="P5316_12490"
     CDS             5184..6767
                     /locus_tag="P5316_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR85818.1"
                     /translation="MKQMRRDYGSCDRMLTVGMLLALAATAAASAAETKPASDARPNI
                     LLAISDDQSYPYASAYGCPGVKTPAFDRIARQGILFTNAICPSPGCAPSRASLLTGRY
                     PWQNEHAGTHASSFPAKLVVYPDLLEKAGYFVGYTGKGWGPGDWEVGGRSRNPAGPEF
                     NKIKIENTTPGVADCDYSANFRLFLEQRPKGMPFCFWYGGKEPHRGYAPGIGLRHGKK
                     PEEARIPPFLPDTPEIRSDILDYCWEIEWFDRHLADMIAALEEAGEFDNTLIVVTSDN
                     GMPFPRAKANVYEYGIHVPMALCWPRRIPGGRTVDDLISFVDLAPTFLDAAGVAPAGL
                     PGGNGRSLMSILTSNEKGLVDTTREMVFSARERHSSSRYQNLGYPQRALRTQRYLYIR
                     NFKPDRWPAGDPRQLNADGSLAPPDSGYCDIDPGPSLSWLITHKTQPQADRFFHLAVD
                     KRAPEELYDIREDPGCLTNLISSAEHAQVLGRLRSQMDDYLRKTNDPRVTGNGDVFET
                     YERYSPIRRFPPPDSSIAP"
     gene            6916..8481
                     /locus_tag="P5316_12495"
     CDS             6916..8481
                     /locus_tag="P5316_12495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRR85819.1"
                     /translation="MCHMKGLPLLAVCVSGCASGPGPLNSIVPADPPLAEPVYVGQAL
                     KIGKPSALEFWVASPVRHHKYPWNIIRMWCPEAAKIDGPDGKPIWSMNILTDPARWEQ
                     TPTGLGAQCDIPRGGRFIRTVRQDGAVLHLTHTFQNHSGAAWKNVHVGCCLQLSAAPD
                     YEDNTGERTYWVLDGQLTPTCRMAITDPGMRGTRGVGQAVEMKDGTRRTLTEGAVFVV
                     SKDNRYVLGYSWQPAAGLFYNRAGIVACNHVQPPDFDIPAAEARSIKGIIFIHEGTLQ
                     EAYRRFLEWKAQPTAADEVVPPLDSSFLKNCAFVCVDIQPGERTVATLESLPKAWRDA
                     GMKPDDLNAATAHKFDVAYPNARKVADACRRIGLPMIFVHWGHRLPDGMDLEPSIRQF
                     NISTFGPDPARWPNHVSSPHARPAEFLGVREGEYVIAKTDHDAFASSNIDYVLRNLGV
                     KNLVFIGGHTDACLGKTSIAAKERGYRTLCVEDATFAGLMSLWRPGLLNSQYDYIITT
                     EEFLKLTESVATK"
     gene            complement(8634..9818)
                     /locus_tag="P5316_12500"
     CDS             complement(8634..9818)
                     /locus_tag="P5316_12500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85820.1"
                     /translation="MAKTCNVALIGQGFMGRTHSNAYLKVAKFFKLPVQPVMHTVFGM
                     KEEDPQAFADRWGWKHSSSDWKSVIRNEEIDFVDIVTPNYMHMPPAMEALEAGKPVAC
                     EKPIAGTLADARAMVEAAKKAKVKTFVWYNYRRCAAVALAHQLVKEGKIGRIYHVRCI
                     YLQEWGGPETPLLWRFQKKLAGSGAHGDLNAHIVDMARFITGDEIVEISGAIAETFIK
                     ERTLVETGPSGGIAGGAKGAKKKGKADVDDAVLFLVRFKKGAVGSFEASRLATGYQNQ
                     NGIEIHGDKGALRFRFEDMNYLDYWDNTIERRLQGWKRIMCTSGGNHPYVGNWWPDAH
                     ILGYEHGFINQAADIMRVLGGEKPEVPLPDFADAYETQRVLEAALLSAESRCAVKMSE
                     VK"
     gene            10173..11339
                     /locus_tag="P5316_12505"
     CDS             10173..11339
                     /locus_tag="P5316_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRR85821.1"
                     /translation="MKITDIELYYLALPQITDAADGTQDTLLVHIGTDAGIEGFGECD
                     ASPLVSMAVYCCPPSHSNIVNIRETILGETVETPEDIRRIQAKVWARGLDIEQIHHAY
                     SGADIALWDILGKKRGLPVYRLLGDQKAYPKRPYGSVLFGDTAQQTRERAGSLAAAGF
                     RAAKFGWGPMGKGTLDDDVALVAAARAGMGPDNEVMIDAGCVWGHDVQTALLRARRFA
                     EYNITWLEEPFHPHAIEAYGRLAEQSPVRLAGGEGSNTVRFAEDLLMHGGVSFVQIDA
                     GRIGGITPAHDVRRLAEKHGAIYVNHTFKSKLSLAAAMHVFAGVERFVYLEYPQSGSP
                     LAENLCKPCLQPGSDGLVRLTEAPGLGVDIDADTVKRFRRPVRIEVDGKTLLEA"
     gene            11396..11995
                     /locus_tag="P5316_12510"
     CDS             11396..11995
                     /locus_tag="P5316_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85822.1"
                     /translation="MGNTWWILITLVGLFAITGANCLTTPVEEPVIFNDGFETGLGSW
                     MQGSDVPEDPNNPGNPVAWAIEQSDAQAVEGRYSARFCLDGGQDDGTIWLVREFDVTA
                     GRVYSVMLSLSLWSESESFNTLAKVAAYAGGKAPDAESDFDVSMAADQVAGWKTYTYV
                     IDATATTNGKIRVAFGISAVWETQLTYYIDDVAIEIEPK"
     gene            12091..12234
                     /locus_tag="P5316_12515"
     CDS             12091..12234
                     /locus_tag="P5316_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85823.1"
                     /translation="MTNVEIRMTNAQIPMTHRWPASAEGLRCGELECRLCEVAWLRST
                     CEE"
     gene            complement(12697..13641)
                     /locus_tag="P5316_12520"
     CDS             complement(12697..13641)
                     /locus_tag="P5316_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR85824.1"
                     /translation="MTAFKEPSRREFLAGSIAAGAAGLGSRLIAADTRPSEPEVKREE
                     TWQIGCYTRPWGGHEYRIALDAIAEAGFKYVGLMTAKSPTNLILSSATTPDEAARVGE
                     EVKKRGLKVANVYAGDFFSTEPDAAVRALRRMIDASAIVGSPGLMLGGSGDPKHNDDY
                     YRTVAACCDYAAQKNIGLSIKPHGGLNATGPQCRRAIEFVGHRNFRLWYDPGNIYYYS
                     EGRLDPVDDAAMVDGLVVGMSVKDYLHPKNVDVTPGTGKVDFAAVMARLKKGGFTRGP
                     LIVETLARGDLPTLLSEAKKARRFVEDLTAGRIAATRP"
     assembly_gap    13904..14003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14127..16115
                     /locus_tag="P5316_12525"
     CDS             14127..16115
                     /locus_tag="P5316_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85825.1"
                     /translation="MTKTHYMLGSLLVAASALTVDSAMAQETRPAPDWPAGWTAAWSA
                     PGAELRPLQILHGVPKGQANPAAMAALKELGLGGIVCNVAFNDYLVSEPNWATLVKAI
                     EACHEVGLLAWIYDEKGYPSGSAGGLVLKENPVLEALALAYDPSRPDPFMIRPSYEHT
                     HASNNFHAARRYINLLDGAAVSTFIDKTHRAYWQRIKRHFGATIPAVFTDEPSLMAVN
                     IGQLGEEIRKKVHVDDPVDPGVQPLPSVPWVADLPDQYRKRYGEDLMVVRLSLFTGDA
                     EADRQVRRRFWGLVADLVSERYFGQIQDWCRRHRVASSGHILWEENPICHTALYGNSL
                     EALRRMDIPGLDMLSSWPQTVVYGGWMAAVLPASAAIIEGRRRVMTEVSDFSQTLAGK
                     PPASLAEMQATAAWQAAFGVTEFTLYYDRAKRPAQEYRAYNDFVGRLNALLRDARLDP
                     DVILYYPIHDLWADYLPVAGPLRLETQCEHSQRLVNSFMRMGQRLATSQQPFVIADHE
                     VLAAAEVRDSKLWIRDHAFHALVLPDGAELPEAASGVVAKFRDAGGRIVRDELKGEAA
                     RFDGENETGASGRLQPASDRIILGRFTRDGREILLLVNVGTQTYTGRVSVRSPGTWWR
                     ADPASGEVKQEDVAQGDEIGLSLPVNATVILVGPAFRR"
BASE COUNT         3175 a         5086 c         4935 g         3036 t
ORIGIN      
        1 gccaactccc caaaccggtc caccagagcg tctgctcgca caagcaaagt cgcatccgcc
       61 cagcatttct gagccgggct cggaaggttg gcttggtcat aagccgcggc cagcaggccg
      121 tacccgtagc ccgcgcgggt gcccaggtgt tcacgaaaga gcgcacggcg gtaatcggcc
      181 ttgacaaggc cgtcggcagc ttggcccatg acaacctctc ggaacagact gaccagctcc
      241 acgtgagcct tgagggaatc cggcaggctc gtacggtcga gacggtcgac catttccacc
      301 gcgtcggccg tctggccggt gcgcaggaga gtcgcggcca gcgccatacg ggtcatcagc
      361 agttgcacgg gattggcgac ggcttcgttt agaatggcct catacacctc ttgcgccgcg
      421 tcataatcat gaccgatttc cgccaggttc cccaggccca gcaaggcctc tgtccgtgcc
      481 ggcgcatggt tgacaggcct ggcgaggagc ttgaccagat gtcgccgagc ttgctcccac
      541 tcctgcagtt gcaggctctc aaatgccgcc agcgtgagtg tggcctgccg ttgccggcgt
      601 accagcttga gaaggacaac ggccacaatg aaaaggagca ccacgtgagg ccaaacccat
      661 aaggggatcc cgctctgggt aaggtactcg ggccgggcca aggccgcccc caacagcagg
      721 ccaatgccca gcaacaccat gcgagagccg tgcagccctc gaagcggcct ggacgtacgc
      781 accagctttt cggcaagctc cttggaaatc atgcatcgtt tgtaaccggt tgccggctcc
      841 atcacaaggc aagcaagaaa acaaagggcg ttcggcgaga atgccagaat gccactcacc
      901 ttcgggtccg gctccgtgca tcaggtttgt tcgaagattt gcccgaaggg gcaggacatc
      961 gttacgtttg tgctgcgtcg ttggcaacag tacgtgtccg gaggccagtg cccatgagaa
     1021 tgctcatact cgtcgccgtc gttgccggta ccttcgtccc ggcggtgtct gatgccgaaa
     1081 acactccgcg gcttcccaac ttcgtgatca ttttcatcga tgacatgggc tatgcggaca
     1141 tcgggccgtt cggcgccaag gactacccga cgctcaacct ggaccgaatg gcacaggaag
     1201 gccgacgttt caccgacttc tatgttgccc agccggtgtg ttcggcttct cgggcctctc
     1261 tgatgaccgg ttgctataac gtgcgggtcg gcatcctcgg agcccttggg ccccgagccg
     1321 accatggcat caacgccgat gagatgacgc tcgccgaggt ctgcaaacag aagggctatg
     1381 ccaccgcctg ctacggcaaa tggcaccttg gccatcaccc caagttcctg ccgacgaacc
     1441 acggcttcga cgactacttc ggcttgccgt attccaacga catgtggccc taccatcccg
     1501 agataacgca cctgcccatg gaggagcgct acaagcgatg gccgcatgtg cccctgatcg
     1561 agggcacgaa gatcatcaac ccgcaaatca ccgacgaaga tcagaacatg ctcaccacgt
     1621 ggtacaccga gcgggcggtg aagttcatcg atcgcaacaa ggaccggccg ttcttgctct
     1681 acgtgcccca ctcgatggtc catgtgccgc tgcacgtgtc cgagaagttc gccggcaaga
     1741 gcaagcgcgg gttgttcggc gacgtggtca tggaggtgga ctggtcggtg ggtgagattc
     1801 tcggggcgat ccggaggcac gggatcgaga agaacacgat ggtgattttc acctcggata
     1861 acggtccgtg gctgagttac ggcacgcacg ccggatcggc caagcccctc cgggaaggca
     1921 aaggcacaac ctttgacggc ggatgtcgcg agcccaccgt gatgtggtgg ccggggacga
     1981 tcccggccgg caccgtctgc attaccccgg ccatgaccat cgatattttg ccgaccatcg
     2041 cccacctgat cggagcaaaa ctgccgaacc acaagatcga cggcaagaac atctggccgt
     2101 tgatcgaagg ccggccggat gcgcgttcgc cccacgaggc gtacttcttc tactggggcg
     2161 aagcgctgga ggccgttcgc atgggccggt ggaagctgca tttctcgcac ccctaccgaa
     2221 ctctaggcaa tcgtaagggc ggcacggacg gcaaacccga gccctacgag caagccgaaa
     2281 tcggcttgac cctgtttgac ctggagctcg acccgggcga gacgaccgat gtcaaggatc
     2341 agcatcctga agtcgtggca gctatcgaga agcttgccga ccgaatgcgc gaagattcgg
     2401 gcgattctgc tcgggggatc accggaactg gacgacgtaa ccccggtcgc ctggaaaagg
     2461 gcgacgaacg tttcgtcgtc aaagatggca agcagcttct ggctcggccg cagtaacgcg
     2521 cagacgggga agcggctccg atggacagat ggtcggcttt ggcaggagtg caaagacact
     2581 ggcaggcgag gtgccagtaa caaatggtgc cgttgacccg tagccggcaa acgctaggat
     2641 gacgctggta agcgctgtct ctcagccctt gccgtgtgca gctctttcgc agtcttttca
     2701 agtgaggaga ccaccatgga cccgaccaaa aaggcactgt cgttgctgga ggagttcaag
     2761 aacttcgccc tcaagggtaa cgtgatcgac ctggcggttg ccgttatcat cggcggcgcg
     2821 ttcgggaaga tcgtcgattc attggtgaag cacctcatca tgcccctgat cagcgtgatc
     2881 atgccgggcg aacagagcta ccttggctgg cactgggtca tcaggggcaa ggaggtccct
     2941 tatgggctgt tcatcggcga ggtggtcaac ttcctgatcg tcgcccttgc cctgttcatc
     3001 ttcatcgtca aattcctggg ctggatcatg aagttcaaga agcaggaggc cgccgctccg
     3061 gccgctccgc cgtcgctcac caaggaccag gaattgctca tggaaatccg ggacctgctc
     3121 aagaaggcct gacagatgcc ggccgtactt gctttctgtg cgaccctttc cctcgtgacg
     3181 cagcagatgg tggccgcgca acgtcccaac gtcgttttta tcctcgtcga tgaccttggc
     3241 tacatggaca tcggggcgaa caatccgaac accttctacg agacgccgaa tatcaatcgg
     3301 ctggcctcga ccggcatgcg attcaccaac gcatacgccg cttgtccggt atgcagcccg
     3361 acacgagcca gcatcctcac cggcaagtat ccggtccgaa cgggcatcac ggactacatt
     3421 aacccggccg gcggcaacca acctcagggc tggaaacgca atacgcgatt gctgcccgct
     3481 ccctacagcg accgcttggc cctggaagag gtcacactgg cggaggaatt gaaggaggcc
     3541 ggctatgcca ccttctttgc cggcaagtgg catctcggcc cggaaggttt ctatcctgag
     3601 gaccagggtt tcgagatcaa caaaggcggc tgcgaatggg gcaaccccca gggcggcaac
     3661 aagtacttct cgccctacgg caacccgcgc ctgcccgacg gaccgcccgg cgagcacttg
     3721 ccccaccggc tcgccaccga aacggtgcgt tttatcgaga cccatcgcga ccggcccttc
     3781 ctggcgtact tgtcattcta ttccgtccac acgcccttga tggcccgcga ggatctcact
     3841 cacaaatacc ttcagaaaaa ggaaaggatc aaacgcacgg ggccggattt cggcaccgaa
     3901 ggcgagagac aggttcgcct cgtgcaagac catgcagtct atgcagccat ggtcgaggcg
     3961 atggatttgg cggtcggaaa ggttcttgac gcgctcgaac gattgcactt ggacgacaag
     4021 accgccgtgt tcttcatgtc cgacaatggc ggcctctcga cctccgaggg ttcgccgacc
     4081 agcaacctcc cgctgcgtgc gggaaagggt tggctctacg aaggcggcat ccgggaaccc
     4141 atgctgattc gttggccggg cgtcgcaaaa ccaggaagcg tctgcgacac gcccgtcacc
     4201 agcaccgatt tctaccccac gatcctcgag atcgccggtt tgccgctccg gccacaccgg
     4261 cacctggacg gcgtgagcct ggtccccttg ctgaaaggcg gcacattgca gcgcggaccg
     4321 ctgttctggc attacccgca ctacggcaac cagggcggct cgccgggcgg ggctattcgc
     4381 gacggcgatt ggaagctgat tgaatggttc gaaagcgggc gcctcgaact gtacaacttg
     4441 cgcgaagaca tcggtgagaa gaacaacttg gcggccgcaa agcccgaccg agtggcctcg
     4501 ctgcacgagc aattgagaga atggcaaaaa cggcttgagg cgaagcttcc cgtcccgaat
     4561 ccggcgtatg ctgagccgaa gactccacgc tgactatgcc cggacgtcga tgttgtttcc
     4621 cagatcctcg gcgaactgag tgacgatggc ctgcatgttc tccatcgccg attcaagcag
     4681 cttggagacg acctgcccat tctgttgcat ggccatcttc aaaaccttgg cccccacagc
     4741 ggtttgcagg cgggccgtgc tcatggccgt cgaaagaccc gcgatatccc cgatcatgct
     4801 catgggagac gtatcggcga gcgtaagatc gggctgaagc aacggggaaa tgtccgataa
     4861 taggccgaga gcctgtcgct caacgggctg ctggtttgtt agcgtaatca gtctgtcaga
     4921 cctttgcccg atgaccggtg tacgctcgcg tgtgcttctt ttcgcaggcg gcttatcggt
     4981 gtcgcgcgtc caatcctttg ggaaccatcg aatgccatca tcgggctctg aattggagag
     5041 atggatctgc gctcggattc cgggtcggca attggcgccg aggcatcggg cggatgtgtt
     5101 gttcttcggt tccgctcgcc ctcgacgata ttgccggatc ggtttaccat gctcggcagc
     5161 ctgactattg tgggagaaca ccgatgaagc agatgagacg cgactacggc tcttgcgacc
     5221 gaatgctcac tgtcggcatg ctgttggctc ttgccgcgac cgctgcagca tcggcggcgg
     5281 agacgaaacc cgcatcggac gctcgtccca acatcctctt ggccatctcc gacgatcaat
     5341 cctaccctta cgcctccgcc tatggttgcc cgggggtcaa aacgcccgct ttcgatcgca
     5401 ttgcccggca agggattcta ttcaccaatg ccatttgtcc ttctcctggc tgtgctccgt
     5461 cgcgagcctc gctgttgacg gggcggtatc catggcaaaa cgagcacgcc ggcacgcacg
     5521 ccagctcatt tcccgcgaag cttgtcgttt atcccgatct gctcgagaag gccggttact
     5581 tcgtgggtta caccggcaag ggctgggggc cgggggactg ggaagtcggt gggcgaagcc
     5641 gcaaccccgc cggaccggaa ttcaacaaga tcaagatcga gaacacgacc cccggtgttg
     5701 cggattgcga ttattcggcc aacttccgtc tttttctcga gcagcggccc aaaggcatgc
     5761 cgttctgttt ctggtacggc ggtaaggagc cgcaccgggg atacgccccg gggatcggtc
     5821 tgcggcacgg aaagaaacca gaggaggcga ggataccccc gtttttgccc gacacgccgg
     5881 agatccgcag cgacatcctg gattactgct gggagattga atggtttgat cgacacttgg
     5941 ccgacatgat cgccgctttg gaggaagccg gcgaattcga caacacgctg atcgttgtga
     6001 ccagcgacaa cggcatgccc tttccacggg ccaaggccaa cgtttacgag tacggtatcc
     6061 acgtcccgat ggccctttgc tggccccggc gcattccggg cggccgcacg gtagacgatc
     6121 tgatcagctt cgtggacctt gccccgacct tcctcgatgc ggccggcgtc gctccggccg
     6181 gactccccgg tggaaacggc cgaagcctca tgagcattct cacgtccaac gaaaagggcc
     6241 tggtcgacac gacccgcgaa atggtcttca gtgctcgtga acgacacagc tcctcacggt
     6301 atcagaacct cggttatccc cagcgggcat tgcgcacgca gcggtatttg tacatccgca
     6361 acttcaagcc cgaccgttgg cccgcaggtg atccgaggca gctcaacgcc gacggcagcc
     6421 tcgctccccc ggacagcggc tattgtgaca tcgatccggg accctccctg agttggctta
     6481 tcacgcataa gacgcagccc caggccgatc ggttcttcca tttggcggtc gacaagcggg
     6541 cccccgaaga gctttacgat atccgcgagg atccgggttg cctgaccaac ttgatcagct
     6601 cggcggagca tgcccaagtg ctcggcaggc ttcgctcgca gatggatgac tacctccgaa
     6661 agaccaacga tccgcgcgta accggcaacg gtgacgtgtt cgagacatac gagcgctata
     6721 gcccgatccg tcgcttcccg ccccccgatt catcgattgc tccgtgagac cgggcaggct
     6781 ccgtcaacgg cgccaaggtg gcaccatcag ccgggatcgc cggaaggcgc cacggttgga
     6841 gccgacgcgg gacaaggaat catccgacgc cgcgacggca tccaatgaag gttccgagaa
     6901 aggaaggtgt cactgatgtg tcatatgaaa gggctgccac tcttggcggt gtgtgtatct
     6961 ggttgtgctt ctggtcccgg tcctctcaat agcatcgtgc ccgccgaccc gccgctggcc
     7021 gaaccggtct acgtgggaca ggcactcaag atcggcaagc cctctgccct cgaattctgg
     7081 gttgcctcac cggtacgtca ccataagtac ccatggaaca tcattcggat gtggtgtccc
     7141 gaggccgcca agatcgatgg ccccgacggc aagccgatct ggagcatgaa catcctcacc
     7201 gatccggccc gctgggagca gacgcccacc ggcttggggg cccagtgcga tatcccgcgc
     7261 ggcggccggt tcatccgcac cgttcgccag gacggagccg tcctgcatct gacccacacc
     7321 tttcagaacc acagcggcgc cgcatggaag aacgtgcacg tcggatgctg cctccagtta
     7381 tcggcggcac cggactatga ggataacacc ggcgagcgaa catattgggt gctcgacggt
     7441 caactcactc cgacgtgccg aatggccatc accgaccccg gcatgcgcgg cacacgcggc
     7501 gtcggacagg ccgtcgaaat gaaagacggc accaggcgga cattgaccga gggtgcggtg
     7561 ttcgtcgtgt cgaaagacaa caggtacgtg cttggatact cgtggcagcc ggcagccggt
     7621 ctgttctaca accgagcggg catcgtggcc tgtaatcacg tccaacctcc cgacttcgac
     7681 attcctgcgg ccgaagccag gagcatcaaa ggcatcatct tcattcacga aggcacgctc
     7741 caggaggcct atcggcgttt cctggaatgg aaggcccagc ccacagcggc cgatgaggtg
     7801 gtgccgccgc tggactcttc gtttctcaag aactgcgcct tcgtctgcgt cgatatccag
     7861 cccggcgagc gaaccgtggc caccctcgaa tcactgccca aagcgtggcg cgacgccggc
     7921 atgaaacccg acgatctgaa cgccgccact gcccacaagt tcgacgtggc ttatcccaac
     7981 gctcgcaagg tcgctgatgc ctgccggcgg atcggcctgc ccatgatctt cgttcactgg
     8041 ggacatcgcc tccccgatgg catggatctt gaaccgagca tccgccagtt caacatcagc
     8101 accttcgggc cggatcccgc caggtggccc aaccatgttt ccagccccca tgcccgtccg
     8161 gccgagttcc tcggtgtccg tgagggagaa tacgtcatcg ccaagacgga tcacgatgcc
     8221 tttgcatcct ccaatatcga ttacgtgttg cgcaatctcg gggtcaagaa cctggtattc
     8281 atcggcggtc ataccgacgc atgcctcggc aagacctcga ttgccgccaa ggagcgcggc
     8341 taccgcacgc tctgcgtcga agatgccacc ttcgccggcc tgatgtcgct ctggcgcccc
     8401 ggcctgctca acagccaata cgactacatc atcacaacgg aggaattcct gaaactgacg
     8461 gaatccgtcg ccaccaagtg agctgcgcca ttgtgcgttt gctcatagca gccggtcccc
     8521 gtgtccaccg gcaacttcac gcaggctgcg cagaagacgg gcggtccacg ccgcttggcg
     8581 tgagccgccc cttcgaaccg acttagccgt ggcgccaggc tgagccggct tcgttatttc
     8641 acctcgctca tcttcaccgc acaacggctc tcggccgaca acaacgccgc ttccagaacg
     8701 cgctgcgtct cataggcatc ggcaaaatca ggaagcggca cctcgggctt ctcgccgccc
     8761 aaaaccctca tgatatccgc ggcctggttg ataaagccgt gctcgtagcc cagaatgtgg
     8821 gcatccggcc accagttccc gacgtaagga tgattccctc ccgatgtgca catgatccgc
     8881 ttccagcctt gcagtcgacg ctcaatggtg ttgtcccagt agtccaggta gttcatgtcc
     8941 tcgaaccgga accgcagcgc gcccttgtcg ccgtggatct cgatcccgtt ctggttctgg
     9001 taaccggtgg ccaaccgact ggcctcgaag ctgcccaccg cccccttctt gaaccgaaca
     9061 aggaacagca ccgcgtcgtc gacgtcggcc ttgcccttct ttttagcgcc tttggcccct
     9121 ccggcaatcc cgccgctggg cccggtctcg accagagtac gctccttgat aaaggtctcg
     9181 gcaatcgccc cactgatctc cacgatctcg tcaccggtaa tgaaacgagc catgtccacg
     9241 atgtgggcgt tcagatcgcc gtgggcgccg ctgccggcga gtttcttctg gaatcgccag
     9301 agcagcgggg tctccggacc gccccactcc tgcagataga tgcaacggac atggtaaatg
     9361 cgtccgatct tgccttcctt cacaagctgg tgggccagcg ccaccgccgc gcaacgccga
     9421 tagttgtacc acacgaaagt cttgactttg gccttcttgg cggcctcgac catcgccctc
     9481 gcgtcggcaa gcgtgcccgc aatgggcttt tcgcaagcca ccggtttgcc ggcctccagg
     9541 gcctccattg ccggcggcat gtgcatgtaa ttaggcgtga cgatatcgac gaagtcgatc
     9601 tcctcgttcc ggatcacgct cttccagtca gaggaggagt gcttccaacc ccagcggtcc
     9661 gcgaacgcct ggggatcctc ctccttcatg ccaaagaccg tgtgcatcac cggctggacc
     9721 ggcagtttga agaacttggc gaccttcaga taggcattgc tgtgcgttcg gcccataaaa
     9781 ccctggccga tcagggcgac gttacaggtt tttgccatcg cgcgtctctc ctttctttcc
     9841 ggcccgcacc gccggcgagc ccgttgcaag cgaggaattg cccttccgat cgcccatgga
     9901 tatagcgacc aaaggcaatg gaatcaagcg cgaccgaacc gcagcgtccc tgcaagggtg
     9961 catccctgaa atagttgcac atgtgtcgac ggttgccgca agcagggcag gggtaaggtc
    10021 gctgcggcga ccgagacctg ccgagagctt cccggatcgt cggcaggctt cgtccgccgc
    10081 gcagattccg accatgccag accttcgtaa cgcgaccgaa aacgggatgc tccccccttg
    10141 ccacaaaacc gctgcacggg caaagtagtg gcatgaagat caccgacatc gagctctatt
    10201 acctggcact gccgcagatt accgatgcgg ccgacgggac ccaggatacc ctcctggtcc
    10261 acattggcac cgacgccgga atcgaaggct ttggagagtg cgacgcgtca cctttggtct
    10321 caatggctgt ctattgctgc ccgccgtcgc acagcaatat cgttaacatc cgcgagacca
    10381 ttctcggcga gaccgtcgag acaccagagg acatccggcg gatccaggcc aaagtctggg
    10441 cccgcggact ggatatcgaa cagatccacc acgcttactc gggagccgac atcgcccttt
    10501 gggacatcct aggcaagaaa cgcggtctgc cggtgtaccg cctccttggt gatcagaagg
    10561 cttatcccaa gagaccctac ggctcggtcc tcttcggtga cacggctcag cagacccgcg
    10621 agcgggccgg atcgctggca gcggccggct tcagggccgc gaagttcgga tgggggccga
    10681 tgggcaaggg aacactcgat gacgatgtcg ccctggtggc cgccgcccgc gcgggcatgg
    10741 ggccggacaa cgaggtcatg attgatgccg gatgcgtctg gggccacgat gttcaaaccg
    10801 ccctgctgcg ggctcgccgc ttcgccgagt acaacatcac ttggcttgaa gagccgtttc
    10861 acccccacgc gattgaagcc tacggccgct tggctgaaca gtcgccggtc agactggccg
    10921 gaggcgaggg ctccaatacc gtgcgattcg ccgaggatct gctcatgcac gggggcgtgt
    10981 cgtttgtcca gatcgatgca gggcgaatcg ggggcatcac ccctgcgcat gacgtccgca
    11041 gactggccga gaagcacggg gccatctacg tcaatcatac ttttaagagc aagctcagcc
    11101 tggcggcggc gatgcacgta ttcgccggcg tcgagcggtt cgtctacctc gaatatccac
    11161 agtccggctc gcccctggcc gagaacctgt gcaaaccctg cctccaaccc ggctcagacg
    11221 gcttggtacg gctgaccgag gcccccggcc tgggcgtcga catcgatgcg gataccgtta
    11281 agcgattccg caggccggtg cgcatcgagg ttgacggcaa gacgctgctg gaggcttgac
    11341 cgtaaggtga ccttgcccgc cggacacctc aaacggagcg acaaaggagg atgcaatggg
    11401 aaacacatgg tggattctga tcacactggt tggcttgttc gcaataacag gcgccaactg
    11461 cctgacgaca cccgtcgaag agcccgtcat cttcaacgac ggattcgaga ccggtttggg
    11521 atcgtggatg cagggttcgg acgtgcccga agaccccaac aaccccggta atccggtggc
    11581 ttgggcgatc gaacagagcg atgctcaggc tgtcgagggc cggtattcag ccaggttctg
    11641 tcttgacggt ggccaggacg acgggaccat ctggttggtc cgcgaattcg atgtcaccgc
    11701 cggcagggta tactccgtca tgctgtcgct ctcgctctgg agcgaatcgg aaagcttcaa
    11761 caccctggcc aaagttgcgg cgtatgccgg cggcaaggcc cctgacgctg agtcggattt
    11821 cgacgtcagc atggccgcag accaggtcgc agggtggaag acatacacct acgtcatcga
    11881 tgccaccgcc accaccaacg gcaagattcg cgtcgcattc ggcatcagtg ccgtttggga
    11941 aacgcaactg acctactaca tcgacgacgt agctatcgag atcgagccga aatagagggc
    12001 gatcaatcct caaacggaga tgcccgggcg gtccgctggc cccggaatcc tttcctgggt
    12061 cttccgcttc ccagccgagc ggagggccga atgacgaatg tcgaaatccg aatgaccaat
    12121 gcccagatac caatgactca tcgatggccc gcgtcggccg aggggctgcg gtgtggcgag
    12181 ctggaatgtc gactctgcga ggttgcttgg ctgcgcagca cctgtgagga atgaagggcg
    12241 aaggcagaaa gcgtgccggg ggagatgaca gggataggcc tgcggcaggt cgacgccctg
    12301 cttggtcggg gcgacttggc ggggctctga cctgccctac ctgctggtgg aggattgcca
    12361 tcgtcatgaa tgccaaagga ttccgggccg agcgagccgc tggccccaga ttcctatctg
    12421 ggacccttct ggtgacgatt cccatcgtca ttcatatgtg aatgcggaag ggattccgca
    12481 ggggatgcgg cccggaaagg attccgggcc gagcggattc atttggaggc gcatgcccac
    12541 gcaaagccgt gggcatggca cccggcggaa aggattcggg ggcgagcggt aaggtttcca
    12601 agggggatgc ccgcgcacgg ccgtgggcat ggccacccgg cacccggcat ggcacgcggc
    12661 acgacaacca cagaacggtc cgggaagggg gtttcgtcac ggccgcgtcg cagcgattct
    12721 gcccgccgtc aggtcctcaa caaacctccg tgccttcttg gcctcgctca gcagcgtggg
    12781 cagatccccg cgagccaggg tttcgacgat cagggggccg cgggtgaagc cgcccttttt
    12841 gagccgggcc atgaccgcgg cgaaatcgac cttgccggtc cccggcgtca cgtccacgtt
    12901 cttggggtgc aggtagtcct tgaccgacat gcccaccaca aggccgtcca ccatggccgc
    12961 gtcgtcgacg gggtcaagcc gcccttcgga atagtagtag atgttgccag gatcatacca
    13021 gagtctgaag ttgcggtgcc cgacgaattc gatcgctctg cggcactgcg gaccggtggc
    13081 gttcaggccg ccgtgaggtt tgatgctcag gccgatgttc ttctgtgcgg catagtcgca
    13141 gcaggcggcg accgtgcggt agtagtcatc attgtgtttc ggatcgccgc tgccgccgag
    13201 catcagaccg ggcgaaccga cgattgcgct tgcgtcgatc atgcgtcgca gagccctgac
    13261 cgccgcatcg ggttcggtcg agaagaaatc gcccgcatag acgttcgcga ccttcagtcc
    13321 gcgtttcttg acttcctcgc cgacgcgagc ggcctcatcg ggtgtggttg cggaagagag
    13381 aatcaagttg gtcggcgact tggcggtcat cagaccgacg tacttgaacc cggcctcggc
    13441 gatagcgtcg agggcgatcc tgtactcatg cccgccccac ggccgggtgt agcagccgat
    13501 ctgccaggtt tcttccctct tgacttccgg ctcggatggc cgtgtatcgg cggcgatcaa
    13561 ccgggaacca agacctgcag ccccggcggc aatcgaaccg gcaagaaact cgcgacgtga
    13621 aggctccttg aacgctgtca tcggtgagaa cctcctcgcg ccagatggta agcgaatccg
    13681 gctccgatct gaaatctgac aatctcaaat ctgcaatccg ataagctgcc gggtggccaa
    13741 cgccctttgg accggcatct tggccgcccg tctcacagag ggccgacgag ggcgtcggcc
    13801 ccacttcacg gcgcgaggct gggctctgag aaaagaaggg ccgccccttt gacttccggc
    13861 tcggatggcc gtgtatcggc ggcgatcaac cgggaaccaa gacnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnngccgccc gtctcacaga gggccgacga gggcgtcggc
    14041 cccacttcac ggcgcgagct tgaaccctcg aaagacaggg accgcccctt tgactttcga
    14101 cctcggggcg caagaatgag cggactatga caaaaacgca ctacatgctt ggatcgttgc
    14161 tcgttgcggc gagcgccctg accgtggatt cggcgatggc gcaggagacc cgcccggcgc
    14221 ccgactggcc ggcgggctgg acggcagcgt ggtctgcgcc gggagctgaa ctgcgcccgc
    14281 ttcagatcct gcatggtgtt ccgaagggcc aggccaaccc cgcggcgatg gctgctctga
    14341 aagagctcgg cctcggcggg atcgtctgca atgtcgcttt caatgactac ctggtctcgg
    14401 agccgaactg ggccacgctg gtcaaggcga tcgaagcttg tcacgaggtc ggactgctgg
    14461 catggatcta tgacgagaag gggtatccga gcggatcggc cggaggtttg gtgctcaagg
    14521 aaaaccctgt gctcgaagct ctcgccttgg cctacgaccc gtcgagaccc gatccgttta
    14581 tgatccgacc gtcgtacgag cacacacacg cgagcaacaa tttccacgcg gcgcggcgat
    14641 acatcaatct gctcgatggc gcggcggtga gcaccttcat cgacaagaca caccgtgcgt
    14701 actggcaacg gatcaagagg cacttcggtg cgaccatccc tgcggtgttc accgacgagc
    14761 cctcgctgat ggcggtcaac atcggtcaac ttggcgagga gatccgaaag aaggtccacg
    14821 tggacgaccc cgtggatccg ggcgtgcagc cgctgccgtc ggtgccatgg gttgccgacc
    14881 tgcccgacca gtaccgcaag cgttatggcg aggacctcat ggtcgtgcgc ctcagcctgt
    14941 tcaccggcga tgccgaggcc gatcggcagg tgcgacgtcg cttttgggga ttagtcgccg
    15001 atcttgtttc cgagcggtac ttcgggcaga tccaggactg gtgtcgccga caccgtgttg
    15061 catcctccgg gcatattctc tgggaggaga atcctatctg ccacacagct ttgtacggca
    15121 acagtctcga ggcattgcgc cggatggaca ttccggggct ggatatgctg tcttcatggc
    15181 cgcagacggt tgtatacggc gggtggatgg ccgctgtttt gcccgcgtcg gcggcgatca
    15241 ttgagggccg ccgtcgcgtc atgaccgagg tgagcgattt ttcgcagacc ctggccggca
    15301 agccccctgc gtcgctcgcc gagatgcagg caaccgcagc atggcaggcg gcgttcggcg
    15361 tgaccgagtt cacgctctac tacgatcgtg cgaagcgtcc ggcccaggag tatcgggcgt
    15421 acaacgactt cgtcggccgg ctcaacgctt tgcttcgcga tgcccggctc gatcccgacg
    15481 tcatcctgta ctacccgatc catgacctgt gggcggacta tctgccggta gccgggccgc
    15541 tgagactgga gacgcaatgt gagcactcgc agcgactggt gaactcattc atgcggatgg
    15601 ggcagcgtct ggcgacttcg caacagccgt ttgtgatcgc cgatcacgag gtgctggcgg
    15661 cggccgaggt gcgcgatagc aagctgtgga ttcgcgatca cgcatttcat gcactggtgc
    15721 tgccggacgg tgcagagttg ccggaggcgg catcaggcgt cgtagcgaag ttcagggatg
    15781 ccggcgggcg gatcgttcgc gacgagttga agggcgaagc ggcccgattc gatggcgaaa
    15841 acgagaccgg cgcgagtggc cgacttcaac cggcgtcgga tcggatcatc ctcgggcggt
    15901 tcaccaggga tggccgcgag atcctgcttc tggtgaatgt ggggacacaa acgtacacag
    15961 gcagggtgtc ggtgcgttct ccgggcactt ggtggcgcgc cgacccggcg agcggtgagg
    16021 tcaaacaaga agacgtcgct caaggggatg agatcgggct ttcgcttccg gtgaatgcga
    16081 cggtcatcct ggtcggtccc gccttccgca ggtagtgcag tggctcggat tcacaaaacc
    16141 ggggttcttg attccgttcc acgcggcgga cggcgtgcaa agcacgccct accgcgccgc
    16201 gtcagggcga gagcggaata cccaggtctt cggcctgctt tcggcaggcg gcgtcggagc
    16261 agagcctgcg gaactcgccc atgagaagcg atgcactctc ctgctctcct tcaatctgga
    16321 gcattctcgc gg
//
LOCUS       DAOTPW010000128        16331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3543_length_16331_cov_5.026849, whole genome shotgun sequence.
ACCESSION   DAOTPW010000128 DAOTPW010000000
VERSION     DAOTPW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16331
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3543_length_16331_cov_5.026849"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="P5316_12530"
     CDS             complement(<1..588)
                     /locus_tag="P5316_12530"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRR85826.1"
                     /translation="MGQLLVDLGVSLILVLGISQFRTPRGARTGNLTAAFALACAVAI
                     VLARHSVAALPVMLVAGAAGIATGCAMALRVRMIQIPAMVAFQHGMGGVAAFLVSFVE
                     LVGQTGRSLSSLSEVSALLGLCVGTATFSGSMVASGKLAGRIRQTPVILAGHNVVLAA
                     LSIGCVLLMLAAGLADRAAVVAAVSATLFVVAAAWG"
     gene            complement(653..985)
                     /locus_tag="P5316_12535"
     CDS             complement(653..985)
                     /locus_tag="P5316_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRR85827.1"
                     /translation="MADSRPKDYIAMLDLLLMLAVFAAAFATGYVLISRVPAMLHTPL
                     MSMTNAISAVTILGALLLFTGHARTMEKTLGAAALAAAAFNLVGGFVITDRMLGLFRK
                     KGSTDPGE"
     gene            complement(989..2146)
                     /locus_tag="P5316_12540"
     CDS             complement(989..2146)
                     /locus_tag="P5316_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRR85828.1"
                     /translation="MIIGIPREILAEERRVSALPETVARYVQMGFTVLVERSAGRGVF
                     RSDEEYAKAGAEIVPDARSLFDRSDVILKVKQPCFNEQLQAHEAALLHKGSILITFLH
                     PAAPANHDMVRTLRDRGVIALTMDGVPRISRAQTMDALTSMSTITGYRSILIAANHFP
                     RFIPMIGTAIGTIQPAQILVVGAGVVGLQAIATAKRLGGVVKVVDIRSEARREAGSLK
                     GTQVVGFEVPDELAHGEGGYARALPPEWLEREREMLRSIVPQVDIIILSALVPGELAP
                     VLITEDMVMNMKPGSVVVDVSIDQGGNCAMTVSGREVMVHDVYICGTANIPGSMAVDA
                     TWLYAHNMLHYVENLFPNGPGRPNLEDEIVLHSLVTIDGKIVHKGALKAMG"
     gene            complement(2651..4591)
                     /gene="mnmG"
                     /locus_tag="P5316_12545"
     CDS             complement(2651..4591)
                     /gene="mnmG"
                     /locus_tag="P5316_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRR85829.1"
                     /translation="MRFDVVVVGGGHAGCEAAWAAAKMGCRTALVTMRIDAIARMSCN
                     PAIGGLGKGQMVREIDALGGLMGLAIDRAGIQFRILNRSKGPAVQAPRAQADRVLYTQ
                     AVIDLLRSVPGLEIIEGIAERLRVCPFAELRRDELGSGFSEDGTWDGEQGPGKEDAVK
                     RPEEEKRYQDGFRVTGVQLADGRELHAKAVVLTTGTFLRGLMHCGPVLTEGGRIGEPA
                     AIGLSESLAALGFTLGRLKTGTPPRIHRDSVDFDRMTPQPGDEQPEPFSFMNDRITQR
                     QVPCWITHTNEQTHRIIRENIHRAPMYSGQIKSRGPRYCPSIEDKVMRFADKSSHQVF
                     LEPEGYESPWMYCNGIPTSLPKDVQDVMVHSIDGFQRAEIVQYGYAIEYDWVPTDQIA
                     STLETKRVAGLFLAGQINGTSGYEEAGGQGLVAGMNAAAKVLGREPVILGRDEAYIGV
                     MIDDLVTRPPDEPYRMFTSRAEYRLHLRSDNADARLTPIGRRIGLVDDARWRAFSAKQ
                     AAIELLETALRSKRAGGQSLLELLKRPTVTLAQLYQDYPELGPKPLLAVLQATEIRAK
                     YAGYLEREQRQIERYRHLESRTIPESFDFASVAELRYEAREKFTRFRPRSLGQAARIS
                     GISPADIATLSLYVVHRQGGKL"
     gene            complement(4787..6112)
                     /locus_tag="P5316_12550"
     CDS             complement(4787..6112)
                     /locus_tag="P5316_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85830.1"
                     /translation="MPIERLGRTVLIVALLSAGAPQSFGQTLVNPSFESHPQNPTGWT
                     AFGDGVTDSDARVYTAPTYGLTAAFHGQRVYGAVKDGSRMNGGIYQRVSGATPGVTYR
                     ARVRIYTFRTGDGEMRCGIGLDPYGGTSPYSANTVWFDPISSNNRWSVIEVTAQAKSS
                     TVTVFLDYGQSGTTGFAINYFDLVELIANPPPPPPCADPEAYMETRLTDRRVDLNEQV
                     EAQYTVPPGYVITGIGARGSDANVSRMRVRQNPLLPDGSLGEPEEVRFGYDPIGGLET
                     NISLPPCYIAVGYGARAAGEFDVMTLAVWARPILADGSLGPVEEFRAGFDAYHPSLER
                     EFLAEPGRVLTGVGLRMQFSDITNIWAETDRYRVHRPAGDFDLDGDVDQEDFAHLQAC
                     VSGQGVTQTDLTCQNAMLDEDDDVDQEDFAIFQACLSGPGILADLHCAN"
     gene            complement(6574..8043)
                     /locus_tag="P5316_12555"
     CDS             complement(6574..8043)
                     /locus_tag="P5316_12555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR85831.1"
                     /translation="MNRGPRLGLVVNEYSQQIGGMSTVARATAEYLYARGYDLHLFTN
                     QQCEPDPVIPTYPILTTDLARDLPRLASFKLDLWHSLNYGYAPLSLRCRPLVLTVHGN
                     DFLSPWVRFKFEKTPLLWRMARRERGRGWTQRLVDWVSLPRVDEIIAVSRFTSDLFQR
                     TCRVRQTPMVVPNGVEEFFLQEGDTRLQLLDSSVPPPCRQPSCPEGAIEDPIRSGASA
                     APLGLTSQCDTVAGGSRPWLHTFAPAGAACGGALGEVRSFGDTPGRCEGVDRRPNRLM
                     SVAALYGWQRKNIDGVIRAMALVGDRLDLEYCIVGDGTGRPALEKLAADLAVSHRVRF
                     LGRLTQEELRAAYASASLFVLTPRPTPGDVEGFGLVYLEAAGSGTPSLGTRFGGTTDA
                     IAEGESGFFADDASPEAIARALEQFFTGRIQFDHETVRDHARRHAWPVVLRRVESIYA
                     RVCPQVREWINSRETTPGVQLRPSPVVEIPPEPAPVLCG"
     gene            complement(8201..9427)
                     /locus_tag="P5316_12560"
     CDS             complement(8201..9427)
                     /locus_tag="P5316_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85832.1"
                     /translation="MHVKLSDLRLLWWLRQRPAGHARLLWQDLRRRKADRRAARQCLE
                     AAIDWLCRAQDQTGSGGVAGFYDLLKGWSPPYPETTGYIVPTFLQAARHLGRQDLRDR
                     AGRMLDWLLTTQMQSGAFPAGLPLDGQPREPRIFNTGQIIQGLVAGYREFGVARYLDA
                     AIAAGEWLCQVQDSDGAWRTASYHGLPHAYHSRVDWPLIELGRQAERTDFVEAARRNL
                     DWVLAQRRSDGWFDQMEMTPGQPPILHPIEYTLGGMWESGELLDDETYRRAVFPAVEI
                     LAEHAIEDRLSGAYGPGFEPVGKSICLTGYAQMAFLWLRIYQRTGLPILQQAAERTLG
                     VLERLADLDNPEPALRGGVRGSYPLTGDYMPLSVVNWGAKFFIDALLLEQSCKIPGGG
                     HRGREVGSSVPEPAMV"
     gene            9621..10484
                     /locus_tag="P5316_12565"
     CDS             9621..10484
                     /locus_tag="P5316_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HRR85833.1"
                     /translation="MTMVAPVAQAKRTAKRWARTCADAVGRLVRSTTPAELTAALRGL
                     GIRPGQTMTVHSSFDGMRYFKGTPFHALQAILDVVGPQGTVVMPTFSFDGRSYDYLRS
                     GPSFDLRRTPAQTGLICELLRRRKDAIRSLSPTHSVAAVGPLARMIVSDHENSLTPFD
                     EHSPWRYLLELNAWMVFVGVPDRLLPVSAYHHFDELLEGEFGVRIYHPERFEVRVLDG
                     EGRERCIGTYAHDPETGRHRDYDRVCEHQARMGVLKRSRVGAISLWGGQLRPLFESYR
                     DLAQKGIKAYR"
     gene            complement(10555..11403)
                     /locus_tag="P5316_12570"
     CDS             complement(10555..11403)
                     /locus_tag="P5316_12570"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR85834.1"
                     /translation="MQTISETANLPAVAQVTEGDQIAAAVRQVKHGGRGFVTNFFAGP
                     KQIESWVNRGELSFLQDERWVWIRRRDRGFFHLYYFAADADAIEESLASVDAIAEGAV
                     LAIDLVGQDAIVAPVARVFRKHGFADRAVLVRMVRSMDPNPMGHGDSPDVEIALPDDA
                     PAILSLLETTFDPYSKQIPDQDQILQAVSAGRILIVRRDAAIAGLVFFEAAGAMATLR
                     YWCVDEAWRDQGIGAKLIKVFFNRCRTCRSIVLWVCTENESAIRRYEHYGFRRTDTKD
                     LILVRR"
     gene            complement(11459..11722)
                     /locus_tag="P5316_12575"
     CDS             complement(11459..11722)
                     /locus_tag="P5316_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRR85835.1"
                     /translation="MTDQVHVRAFIIEQLEEYAKARAMTLPDIADDTDLLESGLVDSM
                     GFVELISAIERRFNCEVDFGQFCSDNFTTLGGLVASVVSPCAA"
     gene            complement(11722..13317)
                     /locus_tag="P5316_12580"
     CDS             complement(11722..13317)
                     /locus_tag="P5316_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRR85836.1"
                     /translation="MPSTDRGLVTGFVKSVEAHDRRPALEVGESILTYAELAARASDL
                     AATIRTFKPGTQPVGAVYGYRSVAAYAGVLGTLLSGKGYVPLHPHFPPARTRYMLMLS
                     GADTVIVGAEAAAKLDQVLEGIDRQLLLICPDTTDVSPFRERWNKHRFVGAGELVRSG
                     KLVVPDRVNTDACAYMLFTSGSTGQPKGVPVSHGNVGSYLEYISKRYDITPEDRASQA
                     FQMTFDVSVHDLFTTWSHGACLCSIPHRQLMAPARFIQDNRLTLWYSVPSVIMFLSRM
                     RMLKPGMFPTLRMSFFAGEVLPAGLASQWQAAAPNSIVENLYGPTEATITITHYRWTP
                     DSMARCLNGNVPIGTVFSTQKAAVVDENLRPVPDGRPGELCLSGSQVARGYLNAPEKT
                     ASQFVHLPGGGDAIWYRTGDRVIRDPDGCIHYLGRIDLQVQIRGYRVELQEVEHVVRR
                     AAGTELAVAVPYPVRDGCAEGIHVFICGRADDVVRKRVLTECGRRLPDYMIPRSVQFR
                     DMMPLNSNGKIDRDVLASSLENV"
     gene            13703..14869
                     /locus_tag="P5316_12585"
     CDS             13703..14869
                     /locus_tag="P5316_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85837.1"
                     /translation="MERVGPNHVVRRGSRRNQAVFFCILGLPALSLAASAVPATEPTC
                     SIRPVPAIGMWYTVWWTKDDQFKHWVNCHVLPTRGPYTAGDPQVIAAHYAQLRDLGVD
                     FLIMDDTNCVGNDGGRINDNIRAWFDFMDRLPSAERIPICIGGGGEMREEGVKGQSRA
                     ADFYWQAWAQRPSYFHLDGKPLLLVDTDDNYGPGDWDDSRFAVRWAYNGDNYESMAKR
                     KTWGWGSYEPAPILAESMSIWPGHRFPKHVAEQGRDPLEESREGGRLYVRMWLRVLKA
                     RPRFVTIADYNNFEEETAIEPSYTWEDPHGHAVPDLYLRITRAYAALRTETPVRGEYY
                     RDEAKPEVFLFDGNRFIHQHAMPRRAVIMLTPTGVLERMRNKLGGGSPSSQPSS"
     gene            15077..>16331
                     /locus_tag="P5316_12590"
     CDS             15077..>16331
                     /locus_tag="P5316_12590"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRR85838.1"
                     /translation="MAPVLQTPEMTTGGAQVRVVLRPETLKPDDKRVITRLFDPHNPR
                     RIRAMFNRVMRLDKPTVARLLASVIEDFGPRHRDIESVLQENFEAVILPHYPDLKISR
                     ERQLLLGSYYTMEYSIESAALFNPAIVPHPDQSGLPAGSVRFLMSLRATGEGHVSSIV
                     FRCGVLSADCDIQFDPPPRYAYTAKPTPDRFFDKHLFFRKLMEMGVYEELVARTLEGL
                     PDQFILDQLREAINQVRQQPDTPRAYRNVAADMLWLAYANYELEFPADCPASEIVIFP
                     ATKSESHGMEDLRLTCFTDGDGQTRYCGTYTAYDGFRTLPMMLETRDFRRFQVHTLNG
                     QHAKNKGMALFPRKVNDCYMMIARHDGENLFLLTSDNIHFWNEARKLQGPVEPWELVQ
                     IGNCGSPLETDAGWLLLTHGVGPVRQ"
BASE COUNT         3178 a         5265 c         4960 g         2928 t
ORIGIN      
        1 gccccacgcc gcggcaacca cgaacagggt cgctgagacc gccgccacaa cagccgctcg
       61 atcagccaga cccgccgcaa gcatcagcaa gacgcatccg atcgacaggg ccgcaaggac
      121 gacgttgtgg ccggcgagaa ttaccggagt ctggcgaatt cggccggcga gcttgccgct
      181 tgcgaccatg ctcccactga atgttgccgt cccaacacag agccccagca gagccgacac
      241 ctcggacagg ctcgacaagg atcgcccggt ttgccccacc aattcgacaa aggatacgag
      301 aaacgctgcc acgccgccca taccgtgctg gaaggcgacc atcgccggga tctgaatcat
      361 tctgacccga agagccatcg cgcaaccggt cgcgatcccc gcagcgcccg ccaccagcat
      421 caccgggagg gccgccacag agtgacgcgc caggacgatc gcgaccgcac aagccagtgc
      481 aaaggcggcc gtcagattgc cggtacgggc gccccgcggc gttcggaatt gcgagatacc
      541 cagaaccagg atcagcgaca cacccagatc gaccagcaat tgtcccatgc gactctccgt
      601 acccgccaag acccttttga gcaaccgcgg cttgccgtcg tatctgcggt actcattccc
      661 ccggatcggt cgaccctttc ttgcgaaaca ggcccaacat tcgatcggta atgacaaatc
      721 ctcccacgag gttgaacgcc gccgccgcca gggccgccgc gccgagcgtc ttttccattg
      781 ttcttgcgtg cccggtaaac agcagcaatg cgccaaggat cgtgaccgcc gagatggcat
      841 tagtcatgga catcagcggc gtgtgcagca tcgccggaac ccgtgagatg aggacgtagc
      901 cggtcgcaaa agcagcggca aagactgcga gcatcaacag gagatccaac atcgcgatat
      961 aatccttggg cctgctgtct gccatggctt agcccatggc cttcagggct cccttgtgga
     1021 cgatctttcc atcgatggtc acgagcgagt gtaggacgat ctcgtcctcg aggttgggcc
     1081 ggccggggcc gttgggaaac aggttctcga catagtgaag catgttatgg gcgtacaacc
     1141 aggttgcgtc gacggccatc gagccgggga tgttcgccgt tccgcagatg tacacgtcgt
     1201 ggaccatgac ttcgcgacct gagacggtca ttgcgcagtt gccgccttga tcgattgaca
     1261 cgtccaccac caccgagccg ggtttcatgt tcatcaccat gtcttcggta atcagcacgg
     1321 gcgctaactc gcccggaacc aatgcgctca gaatgatgat gtcgacctgc ggcacgatgg
     1381 aacgaagcat ctctcgttcc cgctccagcc attccggagg cagagcccgg gcatagccgc
     1441 cttcgccatg ggccagctca tcaggtactt cgaaaccgac aacctgcgtg cccttgaggc
     1501 ttccggcctc gcggcgggcc tcgctacgga tgtccaccac cttgaccacc cctcccagcc
     1561 gcttcgcggt ggcgatggcc tgcaggccga cgacaccagc cccgaccacc agaatctggg
     1621 ccggttgtat cgtgccgatg gccgtcccga tcatcggaat gaagcgaggg aaatggttcg
     1681 cagcgatcag aatggagcga tagccggtga tggtgctcat ggaggtcagg gcgtccatgg
     1741 tctgggccct cgagatgcgc ggcacgccgt ccatggtcag ggcgatcacg ccgcgatcgc
     1801 gaagcgtgcg gaccatgtcg tgattggccg gggcggccgg gtgcagaaag gtgatcaaaa
     1861 tcgagccctt gtggagtaga gcagcctcgt gagcctgcaa ctgctcgttg aaacacggtt
     1921 gcttgacctt gagtatgacg tccgaccggt caaagagcga ccgggcatca ggcacgatct
     1981 cggcccccgc cttggcgtac tcctcgtccg atcgaaacac gcctcttccg gccgatcgct
     2041 cgaccaggac cgtaaagccc atttggacgt atcgggcgac ggtttcgggc aaagctgaca
     2101 cccggcgttc ctccgcgaga atctccctgg gtatgccgat gatcatactc aacacctcat
     2161 ctgacggcga aaacaggcca ttcgcagggt aacatcatca cgactcgtgg ccacttcagt
     2221 caaggcgcgc tggtcagcct ggacggacgg cgatgccggc catgccggcc attctcgtgg
     2281 cgattcctgc ctgcatgccc gcctatagga agcacaaggc gggggcggga aggttgacag
     2341 ccgcccgccc ccttcgcgaa cggtccgctt tgcggcctgc gtcaggcccg tcgagcgatg
     2401 ctcaaacagc aagtgcatgg gttgtgcaat gccgtcggcc gttggccgag cacatactat
     2461 tgccgccgcg gctcaggcga gatcatcagc ggaggggtga tcgattcttt caggctgccg
     2521 atcagcgggg cgaccgttgc cgccttgagt actggcggag gcgcatgcgg cgccacaacc
     2581 ggcggaacaa gcgtctacgc cttggccaag gttccggcgg ccccggcagg gtcgaccgcc
     2641 tcgccggcac ttacagcttt cctccctgcc gatgaacgac gtacaacgac agcgtggcga
     2701 tgtccgccgg gctgataccg ctgatgcggg cggcctggcc caggctgcgc ggacggaagc
     2761 gagtgaattt ctcgcgggcc tcgtagcgga gttcggcaac ggaggcaaag tcaaacgatt
     2821 cggggatcgt ccgggactcc aggtgccgat agcgttcgat ctggcgttgt tcgcgctcca
     2881 gatagccggc gtacttggcc cgaatctctg tcgcctggag gacggcgagt aatggtttgg
     2941 gcccgagctc cgggtagtcc tgatacagtt gggccagcgt gaccgtgggc cgcttgagca
     3001 gctccagcag cgattgcccg ccggcacgct tcgatctcag ggcagtctcc agcaactcga
     3061 tcgcggcttg cttggcggag aaagcccgcc acctggcgtc gtcgactaat ccgatccgcc
     3121 gcccgatcgg cgtcaaccgg gcgtcggcgt tgtcgctgcg caggtgcaag cgatactcgg
     3181 cgcgcgaggt gaacatgcga tacggctcat ccggcgggcg ggtcacgagg tcatcgatca
     3241 tgacgccgat gtaagcctca tcacgaccga gaatcaccgg ctcgcgccca aggactttcg
     3301 cggcagcgtt catccctgcg accaggccct gcccgccggc ctcttcgtaa ccgctggtac
     3361 cattgatctg gccggcaaga aacagccccg caacccgctt ggtttccagc gtcgaggcga
     3421 tttgatccgt gggtacccaa tcgtattcga tggcgtagcc gtactggacg atctccgccc
     3481 gctggaatcc gtcgatcgag tgaaccatga cgtcctggac gtctttgggc agcgaggtgg
     3541 ggattccgtt gcagtacatc caagggcttt cgtatccctc gggctcaagg aagacctgat
     3601 gcgaagactt gtcggcaaac cgcatgactt tgtcttcgat gctcgggcag taccgcggcc
     3661 cgcgcgattt gatctggccg gagtacatcg gggcacggtg gatgttctcg cgaatgatgc
     3721 ggtgcgtctg ctcgttggtg tgggtgatcc agcacggcac ctgacgctgg gtaatgcggt
     3781 cgttcatgaa ggagaatggc tcgggctgct cgtcgccggg ctgaggcgtc atgcgatcga
     3841 aatcgaccga gtcacggtgt attcgcggcg gtgtgccggt cttcaagcgg ccgagggtga
     3901 aacccaatgc cgcgaggctt tcgctcaagc caatggccgc cggctcgccg atccgcccac
     3961 cctcggtgag aaccggtcca cagtgcatca ggccgcgcag gaacgtgccg gtggtgagca
     4021 ccacggcctt ggcgtgcagt tctcgcccat cggccaactg aacgcctgtc acgcgaaaac
     4081 catcctggta tcttttctct tcttcaggcc tcttgactgc atcctctttg cccggccctt
     4141 gttccccgtc ccatgtcccg tcctcgctga accccgaacc cagctcgtca cgccgtagct
     4201 cggcgaaggg gcaaaccctg agtctttcgg cgatcccctc gatgatctct aagccgggta
     4261 ccgagcgcag caagtcgatc accgcctgtg tgtacagcac tcgatccgcc tgtgcacgtg
     4321 gggcctgcac ggccgggccc ttgctgcggt tgagaatccg gaattgaatc cctgcccggt
     4381 cgatggccag ccccatcagc ccgcccaagg cgtcgatttc gcgaaccatc tgccccttgc
     4441 ccagcccccc gatcgccggg ttgcacgaca tccgggcgat ggcatcgatg cgcatggtta
     4501 ccagggctgt ccggcagccc atctttgccg ccgcccaggc cgcctcgcat ccggcgtgtc
     4561 cgccgccaac tacaacaacg tcaaaccgca tcctgtcacc tgcaaccgaa cgttggtgtc
     4621 gggccggccc attttagccg atttggttgc tgaatgggat caagacgggg acgccggtga
     4681 tccggcggag cagccctgcc ggctcgcgca gaaacgcaac gataagttct tgtgcctcag
     4741 caagcccctg gcgcgggaaa cccgccgagg tacctgcatg cgcctttcag ttcgcgcaat
     4801 gcagatcagc aaggataccc ggcccgctca ggcacgcttg gaagatagcg aagtcttcct
     4861 gatcaacgtc gtcgtcctca tccagcatgg cgttctggca tgtcaaatcg gtttgtgtga
     4921 caccctggcc gctcacgcaa gcctgcagat gtgcaaagtc ctcctggtcg acatcgccgt
     4981 cgaggtcgaa atcgcccgcc ggccgatgga cgcgataccg atcggtttcc gcccagatgt
     5041 tggtgatgtc gctgaactgc atccggagcc cgactccggt cagcacccga ccgggctcgg
     5101 ccaggaactc ccgttccagt gatgggtgat aggcgtcgaa gccggctcgg aactcctcga
     5161 ccggaccgag cgaaccgtcc gcgaggatcg gccgggccca tacggccaga gtcatcacat
     5221 cgaactcgcc ggctgctcgg gcgccgtagc cgacggcaat gtagcagggc ggcagtgaga
     5281 tgttggtctc cagtcccccg atggggtcat atccgaaccg aacctcctcc ggctcgccga
     5341 ggctgccatc cggcaagagg gggttttgtc gcacgcgcat tcgtgagaca ttggcgtccg
     5401 agccgcgtgc gccgatgccg gtaatcacgt agcccggcgg aaccgtgtat tgagcctcca
     5461 cctgctcgtt gaggtcgacc cgacggtccg tcaacctcgt ttccatgtag gcctcgggat
     5521 ccgcgcacgg cggaggcggc gggggattgg caatgagttc cacgaggtca aagtagttaa
     5581 tggcaaaccc ggtcgtgccg ctctggccgt aatccaggaa gacggtgact gtcgaagact
     5641 tcgcctgggc tgtgacctcg atcacactcc agcgattgtt gctgctgatc ggatcgaacc
     5701 aaacggtatt tgccgagtag ggcgaagtgc cgccatacgg gtccagacca attccacacc
     5761 gcatctctcc atcgccggtg cggaaggtgt agattcggac gcgggctcgg tatgtcacac
     5821 cgggcgtggc ccctgacact cgctggtaga ttccgccgtt catccgcgaa ccgtctttca
     5881 ccgcgccgta cacgcgctgc ccgtggaatg cagcagtcaa gccgtacgtc ggggcggtat
     5941 agactcgggc atcagaatca gtgaccccgt cgccgaaagc ggtccagccc gtcgggttct
     6001 gcgggtggct ttcaaaactg ggattcacga gggtctgccc gaacgattgc ggcgcacccg
     6061 cgctcagcag ggccacgatc agaacagtcc gcccaagccg ctcaattggc atggtagcct
     6121 ccatctcgac tccgatatgg ttccccacaa ccgaatccgg taaattaagc aatcatccgc
     6181 tctcaggttc cattgtaaac aactttccag tcagacaaaa gccttcggcg gcgatccgct
     6241 ctcgtgtaca cgcgacgttc caataaggac tggggatgca gactctttct gaactcgctc
     6301 tcggctagac gattacctct gagagcccaa ctttggccca tagccaccgg cctgcccgag
     6361 aaccgtttgg ctcggaggtt tgccacggcc accacggcga cgtacgggta gcttccgctg
     6421 gcgccagaat ccactctgtg gccttccgtg tggaggattc ctatcctcat gatcgacgaa
     6481 tgccgaacgg attcggcaag actaggcggc ccggaaggga ttccgggccg agcggagagg
     6541 attccgggcc gagcgggccg agtgggaacg tgatcagccg cacagaaccg gggcgggctc
     6601 gggcggtatc tcgacaaccg gtgatggccg gagttgaaca ccaggcgtgg tttcgcgcga
     6661 gttgatccat tcgcgcacct gcgggcagac gcgggcgtag atcgactcga cccgtcgcaa
     6721 aaccaccggc caggcatggc ggcgggcatg atcgcggact gtctcgtggt caaattggat
     6781 ccggcccgtg aagaactgtt ccagggcacg ggcaatggcc tcggggctgg catcatctgc
     6841 gaagaagcct gactcgcctt ccgcgatggc gtccgtcgtg ccgccgaagc gggtgccgag
     6901 agacggcgtt cctgagccgg ccgcctccag gtagaccagg ccgaagccct ccacgtcgcc
     6961 gggcgttgga cgcggggtca gaacaaacag actggctgag gcgtaggccg cgcgtagttc
     7021 ctcctgggtc agccgtccga ggaaacgcac gcgatgcgag acagccaagt ccgctgcaag
     7081 cttctccagt gcgggccgtc cggtgccgtc gccgacgata caatactcaa gatccaatcg
     7141 gtcgccgacc agcgccatcg cccgaatcac gccgtcgatg ttcttgcgct gccagccata
     7201 caaggccgcg accgacatca ggcgattggg ccgccgatcg actccctcgc atcttccagg
     7261 tgtatccccg aacgaacgaa cctcgccaag cgcaccgccg caagccgccc cggcaggggc
     7321 gaaggtgtgt agccaggggc gcgagccccc ggcaaccgtg tcacattggc tggtcagccc
     7381 cagtggggcg gcagatgctc ctgaacggat cgggtcttct attgcccctt cggggcacga
     7441 gggctgccgg cacggtggag gcacggaaga gtcgagtagc tgaaggcgag tgtcgccctc
     7501 ctgaagaaaa aactcctcga cgccattcgg cacgaccatc ggagtctgtc gcacccggca
     7561 ggtccgctgg aacaggtcgc ttgtgaagcg gctgacggcg ataatctcat ccacgcgagg
     7621 cagcgaaacc caatcgacga gccgctgggt ccagcctcgc ccccgctcgc gtcgggccat
     7681 acgccacagg agcggagtct tctcgaactt gaaacgcacc caggggctca gaaaatcgtt
     7741 tccgtgcacc gtcaggacca gcggtcggca tcgcagcgac agcggcgcat acccgtagtt
     7801 cagcgagtgc cagagatcca acttgaaact ggccagacgc ggcaggtcgc gggccaggtc
     7861 ggtggtcagg atcgggtatg tcggaatgac cgggtcgggc tcgcattgct ggttggtgaa
     7921 caggtgcagg tcgtacccgc gggcatacag gtattcggcc gtcgccctcg caacggttga
     7981 catgccgccg atctgctgcg agtactcgtt aacgaccagt cccaatcgcg gcccgcgatt
     8041 cacaggcaac tcctgcatga atagaggcac tggcggcccc ggcttcgcac aagggccccg
     8101 cggggggcaa gcaagccgcc aggggcgccc tcaaggccag tgacgaactc atggcaggtc
     8161 tgcgtcgcct tcggcgactt cgaccatgcg ctaccgtttc tcagaccatg gccggctctg
     8221 gtacagacga tccgacctcg cgccctctgt gaccgccgcc aggtatcttg cacgactgct
     8281 ccagcagcag cgcgtcgatg aagaacttgg ctccccagtt gactaccgat agcggcatgt
     8341 agtctccggt tagcgggtac gagcctcgca cgccgcctcg caacgccggt tctgggttgt
     8401 ccaggtcggc caatctttcc aacacgccca gagtccgctc agccgcctgc tgcagtatcg
     8461 gtaatcccgt ccgttggtaa atccgcagcc acaaaaaggc catctgcgcg tatccggtca
     8521 ggcaaatcga cttgcccacc ggctcgaagc ccggaccata tgctccggac agacgatcct
     8581 cgatagcgtg ctcggccaat atctcgaccg cggggaacac cgcccgccga tacgtttcat
     8641 cgtcgagcag ttcgcccgat tcccacatgc cgccgagcgt gtactcgatc ggatgaagaa
     8701 tcggcggctg cccgggggtc atctccatct ggtcaaacca accatccgag cggcgctgag
     8761 ccaataccca gtctagattg cgacgggctg cttccacgaa gtccgtccgc tcggcttgac
     8821 ggcccagctc gatcaacggc cagtcgaccc tcgaatgata ggcatgcggg agcccatgat
     8881 acgaggcggt ccgccacgcc ccgtcagaat cctgaacctg gcacaaccac tcgcctgcgg
     8941 caattgcggc gtcgagatag cgggccacgc cgaactcgcg atagccggca acaagtccct
     9001 ggataatctg tccggtgttg aagatgcgag gctcgcgcgg ctggccatcg agcggcagac
     9061 cggccggaaa agctccgctc tgcatttgcg tggtcagcag ccaatccagc atccgaccgg
     9121 cacgatcgcg caggtcttgg cggcccaaat gcctggccgc ctgcagaaac gtcggaacga
     9181 tgtagcccgt cgtctccggg tacggcggcg accagccctt gagcaggtca tagaaccccg
     9241 ccacaccccc gctaccagtt tgatcttgtg cacggcacag ccaatcgatg gcggcctcca
     9301 gacactggcg ggcggcgcgg cgatccgcct tgcggcgtcg caggtcttgc cacaagagcc
     9361 gggcatgtcc ggccggacgc tgccgcagcc accacagaag ccgaagatcc gataacttca
     9421 cgtgcatctg acgggtacct gaacagcttc aggatttatc ggagtgccgg cgactcccat
     9481 gcagctatcg tccgtcagga accaaaacca actgcctctt cccacttcgt atgtggcccg
     9541 cgtcgcccga gtgtccgata agcaagggag aaccgaggcc gttgcccgga ttgaggcggg
     9601 ttgcacgcgc agagactcca atgacgatgg tcgctccagt agcacaagcg aaacgcaccg
     9661 caaagcggtg ggccagaacg tgcgccgacg ccgtgggacg gctcgttcga tccaccacgc
     9721 cggctgagtt gacggcagcc ctgcgcggct tgggcatccg gcccggccaa accatgaccg
     9781 tgcacagttc gttcgacggc atgcggtatt ttaagggcac gccgttccac gccttgcagg
     9841 cgattctcga tgtcgttggc ccccagggca ccgtggtgat gccgaccttt tcgttcgacg
     9901 gccgaagcta cgactacctc aggagcggtc cctcgtttga ccttcgccga acgccggctc
     9961 agaccggcct gatctgtgag ctcctgcgac gccgcaagga tgcgattcgc agcctgagtc
    10021 cgacgcattc ggtcgcggcc gtgggcccgc tggcccggat gatcgtctca gatcatgaaa
    10081 actcgctgac cccgtttgac gaacactcgc cctggcgata cctgcttgaa ctcaatgcgt
    10141 ggatggtgtt cgtgggcgtt ccagaccgcc ttctgccggt gtcggcgtat caccatttcg
    10201 atgaattgct ggaaggcgaa ttcggggtgc ggatttacca cccggaacgt ttcgaggtcc
    10261 gcgtgctcga tggtgaaggc cgcgagcgat gcatcggcac ctatgcccac gacccggaga
    10321 ccgggcggca ccgagactat gatcgagtct gcgaacacca ggcgaggatg ggtgtcttga
    10381 agcgatcgcg cgtcggggca atcagcttgt ggggggggca gctccggccg ttgttcgaat
    10441 cctaccgtga tctggctcaa aagggcatca aggcgtatcg ctgagacatg tcatcgcgat
    10501 accagctctc gagaaagtgg gtcagccgaa tggagggcag tgggttgccg gttgtcagcg
    10561 acgcacgaga atcagatctt tcgtgtccgt ccgacggaac ccgtagtgct cgtatcgacg
    10621 aatggcgctc tcattctcgg tacagaccca caggacgatg ctcctgcagg tccgacagcg
    10681 attgaagaaa accttgatca gcttcgcacc tatcccctga tcacgccatg cctcatcaac
    10741 gcaccagtat cgcagtgtgg ccatcgcgcc ggcggcctca aagaacacca ggcccgcaat
    10801 ggcggcatcc cgccgaacga tcaggattct acccgccgat acggcttgca ggatctgatc
    10861 ctggtccggg atctgcttgg aatacgggtc gaatgtcgtc tcgaggagac tgaggatggc
    10921 aggcgcatcg tccggcaagg cgatttcgac gtccggacta tcgccgtggc ccatcgggtt
    10981 gggatccatg cttcggacca ttctcaccag gacagcccgg tcggcaaagc cgtgcttgcg
    11041 gaacacgcgc gccaccggcg ccacgatggc atcctggccg accagatcga tcgccagcac
    11101 ggccccctcg gcaatcgcgt caacacttgc cagcgactcc tcgatcgcgt ccgcatcggc
    11161 agcgaaatag tagagatgga agaagccgcg gtcgcggcgc cggatccaga cccagcgctc
    11221 gtcctgcagg aatgacagct ctccacggtt cacccagctc tcgatctgct tggggccggc
    11281 gaagaaattc gtcacaaaac cccgaccgcc gtgcttgacc tgccggacag ccgcggcaat
    11341 ctggtcgcct tcggtcacct gagcgaccgc aggcaaattc gcggtttcgg aaatggtttg
    11401 catcggtttt gcccacgctt tgcgaggccc aagcccgcga accggacgga cgccgcattc
    11461 atgccgcaca gggcgacacg accgaagcca ccaggccccc caaggtagtg aagttgtccg
    11521 agcagaactg cccgaaatcg acctcgcaat tgaaccgtct ctcgatggcc gagatcagct
    11581 ccacaaatcc catggagtcg accaggccgc tctccaacag gtccgtatcg tcggcaatgt
    11641 ccggcagcgt catcgccctg gctttcgcgt actcctcaag ttgctcgatg atgaatgcac
    11701 gaacgtgtac ctgatcggtc atcacacatt ctccagactg cttgccagca cgtctcgatc
    11761 gatcttgccg ttggagttca acggcatcat gtcgcggaac tgcaccgatc gcgggatcat
    11821 gtagtccggc agccttcgac cacattccgt cagaactcgt ttgcgcacga cgtcatcggc
    11881 ccgtccgcag atgaacacgt gaatgccttc cgcgcatccg tcgcgaaccg gataggggac
    11941 agcaacggcc agttccgtgc cggcggcccg ccgaaccacg tgttcgactt cctgcagttc
    12001 cacacggtac ccgcggatct gcacttggag atcaattcgc cccaggtagt gaatgcatcc
    12061 gtccgggtcg cgaatgacgc ggtcgcccgt tcgataccag attgcgtcgc cgccgccggg
    12121 gagatgcaca aactgagagg cggtcttctc cggtgcgttc aagtaccccc gtgctacctg
    12181 cgagcccgaa aggcaaagct caccgggccg gccgtccggc accggtcgga gattctcgtc
    12241 tacgaccgcc gccttttgcg tcgagaacac cgtcccgatc ggcacattgc cgttcagaca
    12301 tcgggccatc gagtcaggtg tccatcgata atgtgtgatg gtgatcgttg cttccgtcgg
    12361 cccgtacagg ttctcgacga tcgaattcgg cgcagccgcc tgccattgcg aggccaggcc
    12421 ggcgggcagc acttcccccg caaaaaagct cattcgtagc gtcggaaaca tgcccggctt
    12481 gagcatgcgc atccgcgaca agaacatgat caccgacggg accgaatacc acagcgtcag
    12541 gcgattgtcc tggatgaaac gggcgggagc catcaattgc cggtgcggaa tactgcacag
    12601 gcatgctccg tggctccaag tcgtgaacag gtcgtggacg ctgacgtcaa aggtcatctg
    12661 gaaagcctgg ctggcgcggt cttcgggggt gatgtcatac cgcttggaga tgtactccag
    12721 gtacgagccc acgttgccgt ggctgaccgg caccccctta ggttgcccgg tcgaacccga
    12781 ggtaaacaac atgtaagcac acgcatcggt gttgaccctg tcggggacga ccagcttccc
    12841 agatcggacc aactcaccgg cgccgacaaa tcggtgcttg ttccaccgct ctcggaaagg
    12901 cgagacatcc gtggtatccg ggcaaatcag cagcagctgc cgatcgatcc cctccagtac
    12961 ctggtccagc ttggcagccg cctcagcgcc gacgataacc gtatcagctc ccgaaagcat
    13021 caacatgtat cgggttcgcg caggcggaaa gtgcggatga agtggaacgt agcccttgcc
    13081 ggacagcaga gtgccgagaa caccagcgta agcggcaacg cttcgatagc catacactgc
    13141 cccgacaggc tgagtgccgg gcttgaacgt ccggatggtt gccgccagat cagaggcccg
    13201 cgccgccagt tccgcgtagg tcagaataga ctcaccgacc tccagcgccg gccgccggtc
    13261 gtgggcttcc accgacttga cgaagcccgt aaccaggcct ctatcagtac tgggcattgc
    13321 caaatccccg atccgtcggg cttgttggtc atgacgggcg ggagcgcgcc ttctcgacgc
    13381 ggactacgag actctcctcg tctccgtcgc ataccgggtt atcggcacaa gcaccgggcc
    13441 ggttaagtaa ccaggcgcag gcgggagcga cggatcgtgc ggaccgagaa aacacataac
    13501 gtgcacactg ataagtactt gcgtgcattc gttcccccgg cagatagtcc ggcgacggca
    13561 tcggtcgcgc cggcgttgca ctggcaaggg cggaaggtaa aacaggcgat gtcggccggc
    13621 cgcccggacg catcgagcgc tctggcgcgg tcgaacggca gccggtgcca gattatgacc
    13681 gcagcgcatc gtggagtcct gaatggaaag agtcggacca aaccatgtag ttcgccgcgg
    13741 gagtcgccgc aatcaggcgg tctttttctg tatcctcggc cttccggccc tgtctctcgc
    13801 ggcgtcggcc gtcccggcaa cggagccgac gtgcagtatc agaccggtac cggccattgg
    13861 catgtggtat accgtgtggt ggacaaagga cgatcagttc aagcactggg tcaactgcca
    13921 cgtgttgccc acgcgcggcc cgtacaccgc cggtgatcca caggtcattg ccgcccacta
    13981 cgctcagctt cgcgatctcg gtgttgactt cctgatcatg gacgacacca actgtgtcgg
    14041 gaacgacggc ggccgaatca acgacaacat ccgggcatgg ttcgatttca tggaccggct
    14101 tccctccgcc gagcgcattc ccatctgcat cggcggcggg ggagagatgc gggaggaggg
    14161 agtgaagggc cagagccggg cggcggattt ctactggcag gcatgggcac aacgaccaag
    14221 ctactttcac ctcgacggca agccgctgct gttggtcgat acggacgaca actatgggcc
    14281 gggcgactgg gacgactcgc ggttcgccgt tcgctgggcc tacaacggcg acaactacga
    14341 atccatggcc aagcgcaaga cgtggggatg gggatcatac gaaccggcgc cgatcctcgc
    14401 cgaatcgatg agcatctggc ccggccaccg ctttcccaag cacgtggccg agcagggacg
    14461 ggatccgctg gaagagtctc gcgaaggcgg ccggctttac gtccgcatgt ggctgcgcgt
    14521 tctcaaggcg cgccctcgat tcgttaccat cgccgactac aacaacttcg aggaagagac
    14581 ggccatcgag cccagctaca cctgggagga tccccatggc catgcggtgc ccgacctgta
    14641 ccttcgcatc acgcgggcct atgccgcact ccgaaccgaa acaccggtgc ggggcgaata
    14701 ctatcgcgac gaggcgaaac cggaggtgtt ccttttcgac ggcaaccggt tcatccatca
    14761 gcacgccatg cctcggcggg ccgtgatcat gctcacgccg acaggtgtgc tcgagcgcat
    14821 gcgaaacaaa ctcggcggcg gctcgccttc gtcacagccg tcgtcctgat agggtcgggc
    14881 ccgccaatca gggacatcat cttcacggcg gcacgaggag gtgccggtgc gtcatgtgac
    14941 aggggtctcc ggtcgataga gactatcggt ttgaacccgg gggcaaaccg acgcactggc
    15001 gttgcgtaaa atactcgggt atgttatcgg ccgcttgaag ggatcggtcc gcagtcggag
    15061 gaagcagcaa acggacatgg caccagtact ccagaccccc gaaatgacaa ccggaggcgc
    15121 gcaggtcagg gtcgtgctgc gcccggaaac cctcaagccc gacgacaaga gagtcatcac
    15181 gcgactgttt gatccgcaca atcctcgccg gatacgggcc atgttcaacc gcgtcatgcg
    15241 tctggacaag ccgacggtcg caagactcct cgcttcggtt atcgaggact tcggcccgcg
    15301 acatcgcgac atcgaatcag tcctccagga gaacttcgag gccgtcattc tgccgcacta
    15361 cccggacctg aagatctctc gcgaaaggca actgctcctg ggttcgtatt acacgatgga
    15421 gtactcgatc gaatcggccg ctctgttcaa ccccgccatc gtgccgcatc cggaccagag
    15481 cggtctgccg gccggctcgg tccgcttcct gatgagcctg cgggcgaccg gcgaaggaca
    15541 cgtgtcttcc atcgtgtttc gatgcggcgt gctcagcgcc gactgcgaca ttcaattcga
    15601 cccgccgcct cgatacgcct atacggccaa gccgacgccg gatcgcttct tcgacaagca
    15661 cctgttcttc cgtaagttga tggagatggg cgtctatgag gaactggtcg cccggacgct
    15721 ggaaggcctg cccgaccagt ttatcctcga ccagttgcgg gaagcgatca atcaggtgcg
    15781 ccagcagccg gacacgccgc gggcttaccg caacgtggcc gccgacatgc tctggctggc
    15841 gtatgccaac tatgagttgg agttcccggc cgactgcccg gcctcagaga tcgtcatctt
    15901 cccggccacc aagagcgaga gccacgggat ggaggatctg cgcctcacct gcttcaccga
    15961 tggggacgga caaacgcgtt attgcggcac ctacaccgcc tacgacggct tccggaccct
    16021 gccgatgatg ctcgaaacca gggatttcag gcgattccag gtccatacgc tcaacggtca
    16081 gcacgccaag aacaagggta tggccctgtt tcccaggaag gtcaacgact gctacatgat
    16141 gatcgcccgc cacgacggcg agaatctttt cctgttgacc tccgacaaca ttcatttctg
    16201 gaacgaggcc cgcaagctcc aggggccggt cgagccgtgg gagttggtgc agatcggcaa
    16261 ctgcgggtcg ccgcttgaaa ccgacgccgg ctggctgctg ctgactcacg gtgtcggacc
    16321 ggtgcggcag t
//
LOCUS       DAOTPW010000129        16178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3588_length_16178_cov_5.619178, whole genome shotgun sequence.
ACCESSION   DAOTPW010000129 DAOTPW010000000
VERSION     DAOTPW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3588_length_16178_cov_5.619178"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="P5316_12595"
     CDS             complement(<1..332)
                     /locus_tag="P5316_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HRR85839.1"
                     /translation="MTIKDYQLYHVRPRYLLLKIETDDGLYGWGEPTLEGQTPAVEAA
                     IGQLMRLIKGEDPRRIEHLWQRMYRGGFYRGGPVLCSAISGIEQALWDILGKSLNVPV
                     YQLLGGRVR"
     gene            complement(477..1169)
                     /locus_tag="P5316_12600"
     CDS             complement(477..1169)
                     /locus_tag="P5316_12600"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR85840.1"
                     /translation="MRGDAVGLGLAVTALLVTGGCAADRVLGSGTREDEGFVTIFDGK
                     SMDGWEIIVEPGNEPEKRWRADAFYLEDGALACRGYGFHWFRYKEPLSDFVLRLEFKI
                     AKDTNSGICLRSQEKGAPPFTGFEIQIADDVGKDPHKHGTGAIYDVVTPMYNPSRPVG
                     QWNEMEITLKGSQVVVVLNGLKVIDADFSKLTRPIGKFDFAYANMPRSGYLALQDHWT
                     PIWYRNIRLKRL"
     gene            1421..2254
                     /locus_tag="P5316_12605"
     CDS             1421..2254
                     /locus_tag="P5316_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR85841.1"
                     /translation="MPTMSAFADEVSPDPQVQMDVLEANGIRYIELRGVWGRNVLTFS
                     KQQMADLKKMFEDRGFGVSCIASPIGKVKISDDYRKHFDDFKRATDIALFFDAHYIRI
                     FSYYPPNGADIGDYRREVIDRLAEKVDYVSNLPITLALENESNLFGAYPERCVYLHAA
                     IPSDHLVAAFDPANFVNMNVQDIYNTCWLPLRKFVGYFHIKDFKYGEKEHAVPAGEGD
                     GAIPEILRDAAADGYDGFLALEPHLAKALHSTGETGPELFKVAADALKKIMRDVGWFP
                     V"
     gene            complement(2546..2737)
                     /locus_tag="P5316_12610"
     CDS             complement(2546..2737)
                     /locus_tag="P5316_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85842.1"
                     /translation="MQLWDGNPLDQFYFKVEAAGTDVAADGAQYPMLAVIHGGVFHRQ
                     SNGESAMLATFLLHGNSTG"
     gene            complement(2845..3648)
                     /locus_tag="P5316_12615"
     CDS             complement(2845..3648)
                     /locus_tag="P5316_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRR85843.1"
                     /translation="MDNVSLYWRFLRFLCRWQTILQFKVRVHGVRKVPREGPVLLVCN
                     HQSHMDPVLVSMALPRESCYMGRDTLWANKWFARLITSLNAFPVKRNTADLGAIKESL
                     RRLKNGMSLVVFPEGTRTPDGRIHPMLPGLASIAKKALVPIVPVLIDGMYQAWPKGSP
                     LPRTGNVIIQYGELIRPAEYAGLSAEELVRLLRERLIRMQHRLHQSLPERRLPWYTAR
                     PDVFGVEPPGATVTPAPADDGTNLAGQKANLSGSRNRAPIPKRPGASAR"
     gene            complement(3655..4335)
                     /gene="cmk"
                     /locus_tag="P5316_12620"
     CDS             complement(3655..4335)
                     /gene="cmk"
                     /locus_tag="P5316_12620"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRR85844.1"
                     /translation="MIITIDGPAGSGKSTAARKLAAAMQIPYLDTGAMYRAIALKALD
                     KGIPLDRPDELVAMAESTDIRLDCGPTYVRVVMDGRDVSEDIRSMRVSEYTSQIAQTP
                     GIRRVLVEKQRQIGRQLGSLVTEGRDQGSVVFPDADVKFLVDAEDSKRAERRYHEMLA
                     DGEEVRLEDVLANIRLRDNNDQAQWAPLLASGEAVRIDTTDMTIAQVVARMQEEIRRR
                     RPAGEKTP"
     gene            complement(4587..6035)
                     /gene="miaB"
                     /locus_tag="P5316_12625"
     CDS             complement(4587..6035)
                     /gene="miaB"
                     /locus_tag="P5316_12625"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRR85845.1"
                     /translation="MDKRVYLETMGCQMNKLDSELVLGQLRQLGYLSVDDFRQADVVL
                     LNTCSVRQHAEDKVYSRLGEVARIKRERPEMVVGVIGCMAERDHEGIRRKAPHVDLLC
                     GPGKLNQIADLLAEVLDTRRQVMALEHDRSRRIPKDRRTLEHDSIELLDLSRTAPPDG
                     NVLQAYVRVQRGCDKFCTYCVVPYTRGPERSRPPANIVAEVRKLADMGTREITLLGQT
                     VNSYSYAEGGLTVRFADLLERVHDVPGIERIRFVTSYPGDFTDDILEAMRDLPKVCEY
                     LSLPAQSGSNAVLRRMRRDYTAEEYMDLVERARQMVPGITLSSDFIVGFSGETEQDHQ
                     ATLALVERVRYKNIFMFKYSNRPNTKADKVLPDDVPYKVKQRRHSELMALQHRLSTEH
                     HRSMIGRRVEVLVEGPSKPALRARKAIEAGKEKTSRCVDQLTGHTRGDEIVVFPGPDS
                     LIGKLVTVEVTDASPLTLQGVPADGPGVPPIH"
     gene            complement(6130..8838)
                     /locus_tag="P5316_12630"
     CDS             complement(6130..8838)
                     /locus_tag="P5316_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85846.1"
                     /translation="MQAAHISGRWVLLAAAMLGVASAHSFAASLDDSLFREGLRERGL
                     AEWLDQHLADAPPADATDAMLRKREKLLEEAAVATSVEDRRSATEQASSILRDLLANH
                     PDHPWRLRWQLELARDLLERTAPAAFDALLLYEVPGWSRMQALDLSERAVAILSRLRE
                     DISAAWKSVEALDEASLKHAMVSGSLRLLETLDGRSAYLSAWADFCRVLAADLPTAER
                     KAQMQAILARVAQGSGWIESPDPVQRCGALLMAAVAARLAEEYVQADQYSLQIVKTYG
                     QIGDGRERARVRTASLVAVIEQIRTLRDRNRHEDALSYVDQIRAWAERSRPNDLQASL
                     AVAWAHHMVLVRRAGVTTQPASLLRPEDALQPLESFAKPSAENRDALYALLAGAVQNE
                     DAGVIRTPFGLQLLLGATVAELSLPASTQPTASQPAVNRRLEEVISAARGMLGTLPAD
                     VVPEARGEYLYLLGAAHLTAGRSLEAVATFCDLGEQQPKHDRSARAAAWAVTIAQRML
                     RDTHQDDLRAVRDAFVRAGRLMQKLSPASPATKALPYCIAVALEQNGQLEAAADTYAT
                     VAPDDAQSLLARVGEARCLSTALDQAVARKTLNETQMRQLADRAIRSARQAAAAAQEK
                     KDDTVEHRRLTAQTIILSAALLNHSIIDKSPESLALLEHFEKRFADQSLMLGAVCRER
                     VIALTRLGRLAEARQAAEQCLAVDPEEAGPVLDRLAEAMRDAIERAFDEGDRDVAGRI
                     ARDAVKVGELLSEWSSQRPQRLSAVGRLSIRLRLASSLLHAGQAKEALAVYEECRRAA
                     ATMPSDSLPRAEIQLGRAESLLATGEPAAALPLFAEVWQTLPERSPNWWRALCGNLAC
                     HTRLDHDPKQILQAIQQQRGLSPDLGGPRWQRALEAIQKENEARLR"
     gene            complement(8928..9467)
                     /locus_tag="P5316_12635"
     CDS             complement(8928..9467)
                     /locus_tag="P5316_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRR85847.1"
                     /translation="MSSRSADFDVDRILAGAREAGRSTGRNRPGPLDRPHRRTGLVAI
                     TLNLTPMIDVVFLLLLFFLTASRFGSNEGMLPANLPARSAAVSTEIPRTPIRVRFVAQ
                     PNQPDRCNVTIERFNEAPLPLAELGPSLRKIQEQPGFDADTPVHLMAGNDIRWDHVVN
                     AYNAALAAGYAKVYFAATR"
     gene            complement(9464..9913)
                     /locus_tag="P5316_12640"
     CDS             complement(9464..9913)
                     /locus_tag="P5316_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRR85848.1"
                     /translation="MRRSIGERRPSRGTLVNMTPMIDVTFLLLTFFMLASHFASAEKV
                     KVDLPRPDDNQSVDRRLRDKVIINMLYVEERVKVEPRLQLGPVPVASVGDLGGRLREV
                     GELNPSVQVILRADRRLPYGQVREVMQAVAGAGLTRLQVVTDLEPGR"
     gene            complement(9938..10651)
                     /locus_tag="P5316_12645"
     CDS             complement(9938..10651)
                     /locus_tag="P5316_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRR85849.1"
                     /translation="MAISWLWSGTAGTGVISQTGPSSTVLGKFLVDGGWITWFVLVPL
                     SVVSVALIIHYLIVLRRSTQVPAGLAKALVLAARRGQARDIVEITREDETMLGQAACA
                     GIARLSAGRESARAAITEAVEEQATRLFRRIEYLNVIGNISPMIGLFGTVVGMINAFA
                     RIYSAGGGMPDAAKLAGDISVALITTFWGLLIAIPALSIFSLLRNRIDAFAAESMKLC
                     DSLVSLLCEQQAGSSKAAG"
     gene            complement(10751..11842)
                     /locus_tag="P5316_12650"
     CDS             complement(10751..11842)
                     /locus_tag="P5316_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85850.1"
                     /translation="MREETHALLAGKKQRCRVAATGGVLFAFLALAPTRADIVGLAGR
                     PPAVDVKITTFRGGQIGYRISSGREAAAAIEEIDYLQITNWPLFNLAEKQQSDGHIRQ
                     AAANYGRLLRQTQAPAQGELDRRQLVRCRLLKALDKQGQFDRTVEVYLEILDQEPDLV
                     ERLRPSNLPAAGSTFLQAAADSVAEFINRHGDDEAARFLRRWRETWPTTTTGPASGNG
                     ATASQPAGAVGAEAARQLEEVRHLISSGQFDEALKRIDAILKSPDGAVAELFFWQGKA
                     MLGRAGGNETADAEVDRKRAGLAFMRVVIHFPGHALAPESLYEAGAICRQSGKPEQAA
                     RLWSELVRSYPAADEWVGRASQELERMKK"
     gene            complement(11839..12792)
                     /locus_tag="P5316_12655"
     CDS             complement(11839..12792)
                     /locus_tag="P5316_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85851.1"
                     /translation="MGAIQTSLRTSRSPRHRGAARLARSNMFSWGISILTHLLVFAVL
                     YPLVYSEEVQTRRVVIPEARLAPLVEATPDRPPPAVRINRRLDPTLAPSPDDHLIAAD
                     RLLPSIEADVLPAPLLPTDGTAALSGAGSVIAASQTGPMSTFFGIAGNAYKIVYVVDL
                     SGSLVLNERLVLSEIRRSVQALIPTQQFNIVLTYNEQVFEFRPATLVWANSEHKKQAE
                     AFLSRVVTAGNAREEEAMARAFAARPELIYFLSDGAYHDIDPTNENAPKTSLETRLDQ
                     LNARREVKITVIGFEPLPGPRSVLERIARDHGGNFRTVVPQ"
     gene            13005..14129
                     /locus_tag="P5316_12660"
     CDS             13005..14129
                     /locus_tag="P5316_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85852.1"
                     /translation="MRNMTILTVFIGIAPLAFPAPAADTASAPTGDANLARCMADPPG
                     WVARLDSELAAINKLIESAVEPVDQAAAHLAAANWLVGVANSRAAVRWLTGMQRPEDL
                     GVLASRADQARKHLDKARDALKSVTQARPLEATRISELKLAAESLEPFIKLFAAAGGD
                     ETEAKRKSAFSEAALGLAMARESENPDLSACALLWQSFAWECAGRRDRAMLSLPSALA
                     APEQPAFDFLSRLLRCRLVAEDERHAAAFALALRVRASCNQWFPKEPPDQLAARARLA
                     ALVQYQIGRSWLNQLANPDTKTAADDVKEMLAALRDVLYDRDAPGPIFVPESIIPILV
                     ELPNPPVSQPASYPAGFSTIQDTTMPETTPAPTTAPPAGE"
     gene            14241..14555
                     /locus_tag="P5316_12665"
     CDS             14241..14555
                     /locus_tag="P5316_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HRR85853.1"
                     /translation="MLVVNTENVPGFTIREVKGLVQGNTVRAKHIGRDIVAGLKNLVG
                     GELTGYTELLTEARRQAMERMLAQAQQLGANAVVNVRFATAAVTQGAAELYCYGTAVV
                     IQ"
     gene            14566..15027
                     /locus_tag="P5316_12670"
     CDS             14566..15027
                     /locus_tag="P5316_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRR85854.1"
                     /translation="MFEVLFGVVPFLFLLGLGYGVGTWRERNHLRALSAREKEFTDIR
                     ISTIQTVPDPETVREASLVSGGVVIAADYFKTTAAQIRKLVGGEMGVIRTLVERAQRE
                     ATLRLLAEARRMGATEVWNIRYETSSIRGADKYQAAASVELFVYGTAVVRQ"
     gene            15079..>15544
                     /locus_tag="P5316_12675"
     CDS             15079..>15544
                     /locus_tag="P5316_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85855.1"
                     /translation="MRPAGKPTSPPGEVPGERRCDEHHDGLLSAGGQSIHEEPDIFPD
                     RKPEVLEQDWSCSRCGYNLRGLEAGHPCPECGHRELYRPAPAGSVGYRGWLERKLARG
                     SPRAGWFTAAMLALAGGPLAVLAALFKSGQTGVAGLSTLVLAVVFGPAAEEAL"
     assembly_gap    15545..15554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15555..15909
                     /locus_tag="P5316_12680"
     CDS             <15555..15909
                     /locus_tag="P5316_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85856.1"
                     /translation="AWCVVEVKPYLFRRPGQIRLSTAGAALLFAVIENLIYLNIYVPN
                     PDLALVVWRWTVCVALHTGCTLLASNGLIRVWHRTITELRPPQLSDGLPELAWAIVIH
                     GFYNLVAIFFEFAAR"
BASE COUNT         3006 a         5298 c         4818 g         3046 t
ORIGIN      
        1 cgtacccggc cgccgagcaa ttgatagacc ggcacgttca gcgatttgcc caggatgtcc
       61 cacaaggctt gttcgatgcc ggaaatcgca ctgcacaaga cgggaccgcc gcggtagaaa
      121 ccgcctcgat acattcgttg ccagagatgt tcgatccgtc ggggatcttc acccttgatc
      181 agccgcatca attgcccgat cgccgcttca accgccggcg tctgaccctc cagcgtcggt
      241 tcgccccagc cgtagagccc atcatccgtc tcaatcttaa ggagcaaata acgcgggcga
      301 acgtgataga gctgataatc cttgatcgtc atgtttcact tcgaacccat gctccgactc
      361 gcctgttcaa aggccggtca tatcacgcgc gagccggtcg gtacatgttg cggcttatgc
      421 cgggacaaac cccggggcaa gaaaccacgg ccggcacgtg gccgtcatcg gcacgctcac
      481 aatctcttca accgaatgtt cctgtaccag atcggtgtcc agtggtcctg cagtgccagg
      541 taaccgcttc gcggcatgtt cgcgtatgcg aagtcgaatt tcccgatcgg cctggtgagt
      601 ttcgagaaat cggcgtcgat gactttcaga ccattgagca ccactacaac ctgtgaaccc
      661 ttgagcgtga tctccatctc gttccactgg ccgaccggcc tggatgggtt gtacatcggc
      721 gtgacgacgt catagatcgc tcccgtccca tgcttgtgcg gatccttgcc cacgtcgtcg
      781 gcgatctgaa tctcgaagcc ggtaaacggg ggagcgccct tctcttggct tctcaggcag
      841 atcccgctgt tagtgtcctt ggcgatcttg aactcgagac gaagcacgaa atcgctcagc
      901 ggctccttgt agcggaacca gtgaaaccca tagcccctgc aggctaaagc cccgtcctcc
      961 aggtagaagg catcagcccg ccatctcttt tcgggctcgt tgcccggctc gacaatgatc
     1021 tcccatccgt ccatgctctt gccatcaaag atggtcacga aaccctcgtc ctcacgagtt
     1081 ccgctcccca gcacccggtc cgccgcgcac ccgccggtga ccagcagggc cgtgaccgcc
     1141 aaccccaacc caacggcatc tccgcgcatg atgacctcca ccgcaccggt ccgcgttcgc
     1201 aaacccgccg agcggcagag ggtagtcccc ggtgagcggc gctgtcaagc agcccttgcg
     1261 ctgggctggc cgatctccgg cccagatgtt agattacctt tgacctcttg gggtgcctgg
     1321 gtcaccgccc ggccggccct gatgacaacc tcagccaagt agccggcgac caggggcgga
     1381 tggccgaaat cgacggtccg tgtctgcgag gagaacgacc atgccaacca tgagtgcctt
     1441 tgccgatgaa gtcagtcctg accctcaggt ccagatggac gtcctggagg ccaacgggat
     1501 tcgttacatc gagctccgcg gcgtctgggg ccggaacgtg ctgaccttct cgaagcagca
     1561 gatggctgat ctcaagaaaa tgtttgagga ccgcggcttc ggggtttcct gtatcgcctc
     1621 gcctatcggc aaggtgaaaa tcagcgatga ttaccggaaa cactttgacg attttaaacg
     1681 cgccaccgac atcgctttgt tcttcgatgc gcattacatc cgcattttca gctattatcc
     1741 gccgaacggg gccgacatcg gcgactatcg ccgggaggtc atcgaccgcc tggccgagaa
     1801 ggtggactac gtctcaaacc tgcccatcac gctggccctc gagaacgagt caaacctgtt
     1861 cggagcctac cccgaacgct gcgtgtatct ccacgccgcc atcccatcgg accacctggt
     1921 cgccgcgttc gacccggcca atttcgtcaa tatgaacgtg caagacatct ataacacctg
     1981 ctggctaccg ctccgcaagt tcgtcggcta ttttcatatc aaggacttca aatacgggga
     2041 gaaagagcac gcagtccccg ccggtgaggg cgacggggcc atacccgaga ttctccgaga
     2101 cgcggccgcc gacgggtatg acgggtttct cgcccttgag ccgcatttgg cgaaggccct
     2161 gcactcgacc ggtgaaacgg gtcccgagct gttcaaggtc gcggcagacg cactcaaaaa
     2221 gatcatgcgg gacgtgggct ggttccctgt gtaggtggca gtcgaaacct cggcgcgttg
     2281 cagacacgca tctgtcgctt aaactctttt gaataaaagc cttacagaat caacagcagc
     2341 cttccccgga agactgatgt tgcccaaaaa caacggtcgg tcagtcgtct ggctgtgaaa
     2401 ttcaacttcc ctgacagcca gggccgatac aaggccttgc ccagcaatgg tttgcaaacc
     2461 tcgccagggc tatagcccag gcaggccggc cagtggcccc cgtcttgcgg gagaatcccg
     2521 gcgaagcggg gctaaaagaa gcgattcaac ccgtcgagtt gccatgcagc aggaacgtcg
     2581 ccaacatcgc cgactcgcca ttcgattgcc gatggaatac tccaccgtga ataacggcca
     2641 gcatcgggta ttgcgcaccg tcagcagcaa catcagtacc agcggcctct actttgaagt
     2701 agaactgatc gaggggattc ccgtcccaca gttgcattca ctgctcaagg tctcgctgac
     2761 catacccccc ggcgacggcc acttcccgta tgagggccaa atcagcggta ccgccgaggt
     2821 ggtccgctgt gatcgtctcg agcctcaacg agcagatgcc ccgggccgct tgggtatcgg
     2881 agcccgattc cgcgagccgc tcaggttggc tttctgaccg gccaggttcg ttccgtcgtc
     2941 tgcgggggcc ggtgtcacag tcgcgcccgg cggttccacc ccgaacacgt cgggccgggc
     3001 cgtgtaccag ggcaaccttc gctccggcag gctctggtgc aaacgatgtt gcatacggat
     3061 cagacgttcg cgcaggagtc gcaccagttc ctccgcggaa aggccggcgt actcagcggg
     3121 ccgaatgagc tcgccgtatt ggatgatgac gttgcccgtt cgtggcaacg gcgatccctt
     3181 cggccaagcc tggtacatgc cgtcgatcag gaccggcacg attggcacca aggctttctt
     3241 ggcgatcgag gccaggccgg gaagcatggg atgaatgcgg ccgtcggggg tacgggtacc
     3301 ttccggaaac accaccagcg acatgccgtt cttgagcctt cgcagtgatt ccttgatcgc
     3361 cccgaggtcg gccgtgttcc gcttgaccgg aaaggcgttc agcgaggtaa tcagcctggc
     3421 gaaccactta ttggcccata gggtatcgcg acccatgtag cagctttcgc ggggcagggc
     3481 catcgagacc agcacgggat ccatgtggct ttggtggttg cagaccagca gaaccggccc
     3541 ttcgcgcgga accttgcgca cgccgtgaac gcgcaccttg aactgcagaa tagtctgcca
     3601 gcggcacaag aaccgcagaa acctccagta gagactcacg ttatccatgg gtgatcaggg
     3661 tgtcttttcc cccgcaggcc gccgacggcg gatctcctcc tgcatccgtg cgacaacctg
     3721 ggcgatggtc atgtcggtgg tgtcaattcg caccgcttca cccgaggcca gcagcggagc
     3781 ccactgtgcc tggtcattgt tgtcgcgcag ccggatattg gccaggacgt cttccagccg
     3841 aacctcttca ccgtcggcaa gcatctcgtg gtaacggcgt tccgctcgtt tgctgtcctc
     3901 tgcatcgacc aggaatttca cgtcggcatc gggaaagacc acgctgccct ggtcgcggcc
     3961 ttcggtgacc agggaaccga gctgtcggcc gatctgcctt tgtttctcaa ccagcacccg
     4021 gcggatcccg ggagtctggg caatctgact ggtgtactcg ctgacccgca tgctgcggat
     4081 atcctcgctg acatcacgcc cgtccatgac cacgcgaacg taggtggggc cgcagtcgag
     4141 gcggatgtca gtggactccg ccatggcgac aagttcatca ggcctgtcga gcggtatgcc
     4201 tttgtccagt gccttgaggg caatggcccg gtacatcgcg ccggtgtcga gatacggaat
     4261 ctgcatggcc gccgccagct tgcgcgcggc agtggatttc cccgaaccgg cagggccgtc
     4321 gatggtaatg atcatgccaa tgcctcgcga gagaccgttg gaaccttacg gccggacatc
     4381 tatacgcttt gcacgcatgt ctctggatcg cagagcgctt gggttgcggc ccgcaaaaag
     4441 gcggtccgcc tcgggctgct ggacgaccag gctgtcaggc accgtgtgga atgctgcttg
     4501 ctttcctgac agcccaacag cctaacagtc taacagcctg acagcctctt gccttgctcg
     4561 tattgtgctg ggaaccacag aaatggtcaa tgaatcgggg gcacgccggg cccgtcggcg
     4621 ggcacgcctt gaagggtgag cgggctggca tccgtcacct cgacggtgac gagtttcccg
     4681 atcagcgaat cggggcccgg aaaaaccacg atctcatcgc ctcgcgtatg gccggtaagc
     4741 tggtcgacgc agcgcgaggt cttctcctta cccgcttcga tggctttccg ggccctgaga
     4801 gccggcttgc tcggtccctc gacaagtacc tccacccgcc gaccaatcat cgagcggtga
     4861 tgctccgtcg acagccgatg ctgcagggcc atcaactccg aatgccgccg ctgtttgacc
     4921 ttgtaaggga cgtcgtcagg cagcaccttg tcagccttgg tgtttggccg attggagtac
     4981 ttgaacatga aaatgttctt gtagcggaca cgctcgacca gggccagcgt cgcctggtgg
     5041 tcctgctccg tctcgccgct gaagccgaca atgaaatcgc ttgacaacgt gataccgggt
     5101 accatttgcc gcgccctctc caccagatcc atgtactctt cggcggtgta atcccgccgc
     5161 atccgacgca acacggcgtt cgagccgctc tgggccggca acgacaggta ctcgcatacc
     5221 ttgggcaggt cgcgcatcgc ttccaggatg tcgtcggtga agtcgccggg ataactggtg
     5281 acgaaacgga tgcgttcaat gccgggcaca tcgtggacgc gctccaggag atcggcaaat
     5341 cggacggtga ggccgccctc cgcgtaggaa taactgttga ccgtctggcc gagcaaagtg
     5401 atctcgcgcg tgcccatgtc agccagcttg cggacttctg ccacgatgtt ggccggtgga
     5461 cggcttcgct ccggcccccg ggtgtagggc accacgcaat aagtgcagaa cttatcgcat
     5521 ccccgctgga cgcgaacgta ggcttgcagc acgtttccat cgggcggcgc cgtacgtgac
     5581 aggtccagca actcaatgct gtcgtgttcc agtgtacgcc ggtccttggg gattcgccgc
     5641 gagcggtcgt gttcaagggc catcacctgt cggcgcgtat caagaacctc ggccagcagg
     5701 tccgcgatct gattcagttt tccgggacca cagagcaggt caacgtgcgg agccttgcgc
     5761 cggatgccct catgatcacg ttcggccatg cagccgatca cgccgacaac catttcgggg
     5821 cgctcgcgct tgatgcgggc cacctcgccc agtcggctgt agaccttgtc ttcggcatgc
     5881 tgacggacgc tgcaggtgtt gagcagcacg acgtcggcct ggcggaaatc gtcgacggaa
     5941 aggtacccca gttggcggag ttgccccaac accagctcgc tatcgagctt gttcatctgg
     6001 caacccatcg tttccaggta gacgcgcttg tccatggcgt gcgccactct gctcagccgg
     6061 caaagtccgg tcgttcgaca tccagaccaa ccattctacc tgaagcggaa ggtgtcccaa
     6121 acggccgatt cagcgcaggc gggcttcgtt ttccttctga atcgcttcca gggcacgctg
     6181 ccatcgcggg ccgcccaggt cgggcgacag tcctcgctgc tgctggatgg cctgcagaat
     6241 ctgtttgggg tcatggtcta agcgggtgtg acaggccaga ttgccgcaga gtgcccgcca
     6301 ccagttgggt gatcgctcgg gaagtgtctg ccagacctcg gcaaacagcg gcaaggctgc
     6361 ggccggctcg ccggtcgcca gcaaggactc agcccgccca agctggatct cagcccgcgg
     6421 gaggctatca gaaggcatcg tcgcggccgc tcggcggcat tcctcgtaaa cagcgagagc
     6481 ttccttggcc tgtccggcgt gaagcagcga cgaagccaac cgcaagcgaa tactcagccg
     6541 cccgacggcc gagagcctct ggggacgttg actggaccac tccgagagca gctcgcccac
     6601 tttcacggca tcgcgcgcga ttctgccggc cacgtcacgg tcaccttcat cgaaggcacg
     6661 ctcgattgcg tccctcatgg cctcggccaa ccgatccagg accggaccgg cctcttcagg
     6721 gtcgaccgcc aagcactgct ccgccgcctg tcgagcctcg gcaagccgcc ccagacgcgt
     6781 cagggcgatc acccgctcgc gacagacggc accgagcatc aacgactgat ccgcaaaacg
     6841 cttttcgaaa tgctccagca gagccaggct ctcgggggat ttgtcgatta tcgagtggtt
     6901 cagcagggcg gctgacagga tgatcgtctg agcggtcaag cgacggtgct ccacggtgtc
     6961 gtcttttttc tcctgcgcgg ctgccgccgc ctgccgggcc gaccggatag cccggtcagc
     7021 aagttgtcgc atctgcgtct cgttcaacgt ctttcgggcg acggcctggt ccagcgcggt
     7081 gctcaagcat cgggcctcgc cgactcttgc gagcagcgat tgcgcatcgt ccggcgcaac
     7141 cgttgcatac gtgtcagcgg cggcctccaa ctgaccgttt tgctcaaggg caaccgcgat
     7201 gcagtaggga agcgccttgg tcgccggcga agccggcgac agcttttgca tcagtcgacc
     7261 ggcgcgaacg aaggcgtcgc gaaccgcccg cagatcatcc tgatgcgtgt cgcgaagcat
     7321 gcgttgcgca atcgtgaccg cccacgcggc agctcgtgcg ctgcgatcat gcttcggttg
     7381 ctgctcaccc agatcgcaaa acgtcgccac agcctccagc gaccggccgg ccgtcaggtg
     7441 tgccgcccca agcaggtaga gatactctcc tcgcgcctcg ggcacgacgt cggccggcag
     7501 cgtcccaagc atgcctcggg cggcggagat cacctcctcc aatcgccggt tgacggcggg
     7561 ctgcgaagcg gtcggctggg tggacgccgg cagggaaagc tccgccaccg tcgcgccgag
     7621 gagcagttgc agaccaaagg gtgtccggat cacgccggca tcctcgttct gcactgcccc
     7681 cgccagaaga gcgtacagtg cgtcgcggtt ctccgccgag ggctttgcga acgattcgag
     7741 cggctggagc gcgtcctcgg gtcgcagcag gctcgccggt tgcgttgtga cgccggctcg
     7801 gcggaccaga accatgtgat gggcccacgc aactgccagc gaagcctgaa gatcattagg
     7861 cctcgatcgc tccgcccagg cgcgtatctg atcaacataa gagagtgcat cctcgtggcg
     7921 gttgcggtct cgcagcgtcc ggatctgctc gatcacggcc accagcgagg cggttcgcac
     7981 gcgggcacgc tcgcgaccat ctccaatctg cccataggtc ttgacgatct ggaggctgta
     8041 ttggtccgct tggacgtact cttccgccag acgcgccgcg acggcggcca tcagcaacgc
     8101 gccgcatcgc tgaaccggat cgggcgactc gatccaaccg cttccctggg ccacacgcgc
     8161 cagaatagcc tgcatctgag ctttccgttc ggcggtcggc aggtctgcag caaggacgcg
     8221 gcagaagtcc gcccaagccg acaggtacgc cgatcgcccg tccagcgttt caagcaggcg
     8281 cagcgaaccg gagaccatcg catgtttcag cgaggcttcg tccagagcct cgaccgattt
     8341 ccaggccgcc gagatgtctt cacgcaatcg gctcaggatc gccacggccc gctcgctcag
     8401 atccagggct tgcatgcggc tccagcccgg tacctcgtac agcaacaagg catcaaacgc
     8461 agccggggcc gtacgttcca gcagatcacg ggccagctcc aattgccatc gcaaccgcca
     8521 cgggtgatcc ggatggttgg ccagcagatc acggagaatc gagcttgcct gctccgtcgc
     8581 actccgccgg tcctcgaccg acgtggccac tgcggcctcc tccagaagct tttcccgctt
     8641 gcgaagcatc gcgtcggtcg cgtccgccgg cggtgcgtcg gcgaggtgct gatcgagcca
     8701 ttcggccagc cctcgttccc ggaggccctc gcgaaagagc gagtcatcaa gggacgccgc
     8761 aaaggagtga gccgaggcga cgccgagcat ggccgccgcc aatagaaccc aacggccgga
     8821 tatgtgtgcg gcttgcacgc atctttccgg atcgcggtac gcttgggttg cggtccgcaa
     8881 aaaggcggtc cgccttggga gcttcacgga atgtctttgt gtccgtttca tcgcgtcgcg
     8941 gcaaaataga cctttgcata accggccgcc aacgcggcgt tgtaggcgtt gaccacgtga
     9001 tcccatcgga tgtcgttgcc tgccatgaga tgcacgggtg tgtcggcgtc gaagcccggc
     9061 tgctcctgga tcttgcgcag cgatggcccc aattcggcca gaggcagcgg cgcctcgttg
     9121 aatcgctcga tcgtcacgtt gcaccggtcc ggctggttcg gctgggccac gaaacgcacg
     9181 cgaatcggcg tccggggaat ctccgtggaa accgcggcac tgcgggccgg cagattggcc
     9241 ggcagcatac cctcgtttga cccgaagcga ctggcggtca ggaagaacaa caacaacagg
     9301 aacaccacgt cgatcatcgg tgtaagattg agcgtgatgg cgaccaggcc cgtccgccgg
     9361 tggggtctgt caagcggtcc cggtcggttg cgacccgtgg aacggcccgc ctcgcgcgcg
     9421 cccgcgagaa tgcgatcgac gtcaaaatca gcactgcggc tgctcatcgg cccggctcca
     9481 gatcggtcac cacctgcagg cgggtcaggc ccgcgccggc gaccgcctgc atcacctccc
     9541 gcacctgtcc atacggcaac cgtcggtcag cccgtaagat cacttgcacg ctcgggttca
     9601 gttctcccac ctccctcagc ctgccgccca agtcgcccac cgacgccacc ggaaccggcc
     9661 cgagttggag gcgcggctca accttaaccc tttcctcgac gtacagcatg ttaatgatca
     9721 ctttgtccct cagccgacga tcgaccgact gattgtcgtc cggccggggc aggtcaacct
     9781 tcaccttctc cgccgaggcg aaatgactgg ccagcatgaa gaacgtcagc aacagaaacg
     9841 tgacgtcgat catcggcgtc atgttcacca gcgtacctcg gctcggacgt cgttccccaa
     9901 tggatcgtct catgcctgtt tcctgttcca gacgttttca gcccgcggcc ttggacgaac
     9961 ccgcctgctg ttcacagagc aacgaaacca gactgtcgca aagcttcatg ctctcggccg
    10021 caaacgcgtc gatccggttt cgcagcaggg agaagatcga cagcgcgggg atggcaatca
    10081 gcaatcccca gaaggtcgtg atcaaagcga cagagatgtc gccggccagc ttggcggcgt
    10141 cgggcatccc cccgcccgcg gaatagatcc gagcgaatgc gttgatcatg ccgaccaccg
    10201 tgccgaacag gccgatcatc gggctgatgt tgccgatcac gttgaggtac tcgatacggc
    10261 ggaacagccg tgtcgcctgc tcctcgaccg cctcggtgat ggcggcacgc gccgactcgc
    10321 ggccggcaga cagtcgtgcg atgcccgcgc aggccgcctg tccgagcatg gtctcgtcct
    10381 cccgcgtgat ctcgacgatg tcccgcgcct gtccccgccg ggcggccaga accagcgcct
    10441 tggccagtcc ggccggcacc tgcgtcgatc ggcgcagaac gatcagatag tgaataatca
    10501 gcgctacaga gacaaccgac aacggaacga gaacaaacca ggtgatccag ccgccatcca
    10561 ccaggaattt gccgagcacc gtgctcgacg ggccggtctg agaaatcacc cctgttcccg
    10621 ctgtgccgct ccagagccat gagattgcca taggttccct cgcgatgttc tgcgtgccag
    10681 gcacgggcgc cccgcctgac caagcctccc aggccccctc cccaaccgac gaccgaagac
    10741 ggtccactga ctactttttc atcctttcca actcctgtga tgcccggccg acccactcgt
    10801 ccgcggccgg gtaactgcga accagctccg accacaacct ggcggcctgt tcgggctttc
    10861 cgctctgccg gcagatcgct cccgcctcgt aaaggctctc cggtgccaag gcatgacccg
    10921 gaaagtgaat cacgacccgc atgaacgcca aacccgcacg tttgcggtca acttcggcgt
    10981 ccgccgtctc gttgccaccc gcccgtccga gcatcgcctt gccctgccag aagaaaagct
    11041 ccgcgacggc cccgtcgggc gacttgagaa tcgcatcaat ccgtttgagg gcttcatcaa
    11101 attgcccgct gctgatcaaa tgccggactt cttccagctg cctggccgcc tcggcaccga
    11161 cggccccggc cggttgagaa gccgtcgctc cattgccgga tgccggtccg gtggtcgtgg
    11221 tcggccaggt ctcgcgccat cgccgcagaa accgagcggc ttcgtcgtcg ccgtgacgat
    11281 tgatgaactc agccaccgag tcggccgccg cctgaaggaa ggtcgatccc gcagcgggga
    11341 ggtttgacgg cctcagccgc tcgaccagat ccggctcctg atcgaggatc tccaagtaca
    11401 cctcgaccgt ccgatcgaat tgcccctgct tgtcaagggc cttcaacagc cgacagcgaa
    11461 ccagttggcg gcggtccagt tcgccttggg cgggtgcttg ggtttgcctg agaagccgcc
    11521 catagttggc tgccgcctgc cggatatgtc cgtcgctctg ctgcttttcg gcaaggttga
    11581 acaacggcca gttcgtgatc tgcaggtagt cgatctcttc aatggcagcg gcggcctctc
    11641 gtcccgacga aatgcgatac ccgatctgcc cgccgcggaa agtcgtgatc ttcacgtcga
    11701 ccgccggtgg tctcccggcc aggccgacga tgtcggcccg ggtcggcgcg agcgccagaa
    11761 acgcgaaaag aacaccgccg gttgccgcca ctcggcatcg ctgcttcttt ccggcgagca
    11821 atgcgtgcgt ttcctctctc actgtggcac caccgttcga aagttgccgc catgatcgcg
    11881 cgcgatgcgc tcgagaacgc tgcgtggccc cggcaacggc tcgaagccga tcacagtaat
    11941 cttcacctcg cgacgggcat tcaattggtc caatcgagtc tccagcgaag tcttgggggc
    12001 gttctcgttt gtcggatcaa tgtcgtgata agccccgtcc gaaaggaaat agatcagttc
    12061 cggccgtgcc gcaaaggccc tcgccatcgc ctcctcttcc cgggcgttcc ctgcggtcac
    12121 aactctgctg agaaacgcct cggcctgttt cttgtgctcg ctgttggccc aaaccaatgt
    12181 tgccggtctg aactcgaaga cttgttcatt gtaagtcagc acgatgttga actgctgcgt
    12241 tggtatcagc gcctggaccg atcttcgtat ttcgctgaga acaagcctct cgttaagcac
    12301 gagcgagccg gacaggtcca cgacataaac gatcttgtaa gcgttgcctg cgatgccgaa
    12361 aaacgtgctc atcggaccgg tttgacttgc ggcaatgaca gaacccgcac cggacaacgc
    12421 cgcggtcccg tcggtcggca aaagcggtgc cggcaagacg tccgcttcaa tggagggcag
    12481 gagccggtcg gcggcgatca agtggtcgtc ggggctgggg gcaagggtcg ggtccagccg
    12541 gcggttgatc cgaacagcgg ggggcggccg gtcgggggtt gcctcaacca ggggtgccag
    12601 ccgcgcctcc gggataacca cccggcgggt ctgcacctcc tcgctgtaaa ccagcggata
    12661 cagaacggcg aagacaagca gatgagtgag gatcgaaatg ccccaggaga acatgtttga
    12721 ccgggcaagc cgcgccgccc ctcgatgccg gggacttctg gaagtccgga gtgacgtttg
    12781 aatcgctccc atgggtccgt ccaccagtct cctgcaacgg tcaacctcgt ctcggccgag
    12841 ccgtccggac ttccggccga ccaagctctt aatgcggagt gtatttttcg ccggacggac
    12901 cgtcaaatgg tgaaccaacc gatggaatgt ggtatacggt atgcaccgtg tcgggcaaga
    12961 ggcggtatca ctctggcggg gaacagacgg cgaagagaga taacatgaga aacatgacca
    13021 tcctgacggt tttcatcggg atagctccct tggctttccc ggctccggcc gctgatacgg
    13081 cttccgcgcc gacgggcgac gcgaatctgg cccgctgcat ggctgatccg cccggatggg
    13141 tcgcccggct cgactccgag ctggcggcga tcaacaagct tatcgaatcc gctgttgagc
    13201 ccgttgacca ggctgccgcc catctggcgg cggcaaactg gctggttggc gtggcgaatt
    13261 ctcgagcggc ggtccgctgg ctgaccggaa tgcagcggcc cgaagacctt ggagtcctcg
    13321 cctccagagc cgaccaggct cgcaagcatc tcgacaaggc ccgtgatgcg ctcaaatcgg
    13381 ttacccaagc caggcctctc gaggcaacca ggatctccga attgaagctc gctgcagaat
    13441 cgctggaacc gttcatcaag ctgtttgccg cggccggtgg cgacgaaact gaggcgaaac
    13501 gcaagtctgc tttttccgag gccgccttgg gcctcgctat ggcacgcgag tcggagaacc
    13561 ctgacctctc ggcctgtgca ctgttgtggc agtccttcgc ctgggaatgc gcgggtcggc
    13621 gcgacagggc gatgctttcc ttgccaagtg ccctggccgc acccgaacag cccgcgttcg
    13681 acttcttgag ccggcttctc cgttgccgtc tggttgctga agacgaacgt cacgccgcgg
    13741 cgttcgcgct cgccttgagg gtccgggcgt cctgcaatca gtggttcccc aaggagccgc
    13801 cggatcaact ggccgcccgc gcccgcctgg cggccctggt tcaataccag atcggccgct
    13861 cctggctgaa ccaactggcc aaccccgaca caaagaccgc cgccgatgac gtcaaggaga
    13921 tgctcgctgc cctcagagat gtgctttatg accgtgacgc tcctggaccg atcttcgttc
    13981 ctgagagtat cattccgatc ctcgtcgaac tgccaaaccc ccctgtttca cagccggctt
    14041 catatccggc cggattctct acgatacaag ataccacaat gccggaaacg acccctgccc
    14101 cgacaacagc tcctcccgcc ggtgaatgag gcggcttctc catcggacgc acatacgcac
    14161 cgtctcattg acaccggtat gcctttgccc tcacaattca ggcaacttta tctcgtcaga
    14221 ctgatttcgg aggtcatctc atgctcgtgg tcaataccga aaacgttccg gggttcacca
    14281 ttcgcgaagt caaggggctg gtgcaaggca acacggttcg tgccaaacac atcggtcggg
    14341 acatcgttgc cgggttgaaa aacctcgtcg ggggtgagct caccggctac accgagctgc
    14401 tcacggaagc ccgccggcag gccatggagc gaatgctcgc ccaggcccag cagttgggcg
    14461 ccaacgccgt cgtcaacgtg cggttcgcca ccgccgctgt gacccagggt gcggccgaac
    14521 tgtactgcta cggcacggcc gtggtgattc aataggagcg gccgtatgtt tgaagtcctc
    14581 ttcggcgttg taccgttcct ctttctcctg ggactcggat atggcgtggg cacttggcgc
    14641 gaacgcaacc atcttcgcgc cttgtctgcc cgcgagaaag agttcaccga tatccgaatc
    14701 agcacgattc aaaccgtccc cgaccccgaa acggtgcggg aagcgtcgct ggtcagcggc
    14761 ggagtggtga ttgccgccga ctacttcaag acaaccgccg ctcagattcg caagcttgtc
    14821 ggaggggaaa tgggagtaat ccgcacgctg gtcgagcgag cccagcggga agcgacactt
    14881 cgcctgctgg ctgaggcccg ccgcatggga gcaaccgagg tgtggaatat ccgctatgag
    14941 accagcagca tccgtggtgc cgacaaatac caggcggcgg ccagtgtcga gttgtttgtc
    15001 tatggaacag ccgtcgttcg tcagtgatgt ggcatgccag acccctcaca cgatccatcc
    15061 gtgtttcacg agccacacat gcgtccggca ggcaagccga caagcccgcc cggcgaggtt
    15121 ccgggtgaac gccggtgtga tgaacaccat gatggactgc tttccgccgg cggacaaagc
    15181 atccacgaag aaccggacat cttccccgac cgcaagccgg aggttctgga gcaggattgg
    15241 agctgcagcc gctgcgggta caacctgcgc gggctcgaag cgggtcatcc atgccccgag
    15301 tgcggccacc gcgaactgta ccgcccggca cccgcgggtt ccgtcggcta ccgcggctgg
    15361 ctggaaagaa agctggcccg cggttcgccc cgcgccggct ggttcacggc cgccatgctg
    15421 gcactcgccg gcgggcccct cgccgtcctc gcggcattgt tcaagagcgg acagaccggc
    15481 gtcgccggac tgagcaccct ggttctcgcc gtcgttttcg gcccggcggc cgaggaggcg
    15541 ctcannnnnn nnnntgcttg gtgtgtcgtc gaggtcaaac cctatctttt ccgccggccc
    15601 gggcagatcc gcctgtcgac cgcaggtgcg gccctgttgt ttgcggtcat cgagaacctg
    15661 atctatctga acatctacgt gcccaaccca gaccttgcgt tggtcgtctg gcgatggacg
    15721 gtctgcgtgg ccctgcatac cggctgtacc ctcctcgcct caaacggctt gattcgcgtc
    15781 tggcaccgga ctatcaccga gcttcgccca cctcagctgt ccgacggcct gcccgaactg
    15841 gcttgggcga tcgtcattca cggcttctac aacctggtgg cgatcttctt cgagttcgcg
    15901 gcgcggtagc gcggcagtgc cggctgaatc aagcaatgca aagcgcgttc gagccgagac
    15961 cggacaaacc gcacgctgcg gaaccgaaag caaggttgtg ttctccattc catcgcgttc
    16021 agaaccttac ggccagacat ccgtccggct tgcgcgcctg tttcttgatc ccacagcgcc
    16081 gggtgccatg ccgacggctt tgcgtgtgca tgcgcaggca ggaccgctcg gcccggaatc
    16141 ctttccgggc cgcatccccc gcccgctcgg cccggaat
//
LOCUS       DAOTPW010000130        15718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3720_length_15718_cov_5.629764, whole genome shotgun sequence.
ACCESSION   DAOTPW010000130 DAOTPW010000000
VERSION     DAOTPW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15718
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3720_length_15718_cov_5.629764"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..601
                     /locus_tag="P5316_12685"
     CDS             188..601
                     /locus_tag="P5316_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRR85857.1"
                     /translation="MSKDIWAAQNKSKTKDQTELTETEFLTLDLLADRQPQTVGDLQK
                     QIGILPTQMSRVIRALESKSDGPLIRCQINQQDKRKVDVELTEAGRQAHQAYRQFKLG
                     SIQRLLTGLSEQDRLELVRILRLVRQEARKVLSQK"
     gene            767..1090
                     /locus_tag="P5316_12690"
     CDS             767..1090
                     /locus_tag="P5316_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85858.1"
                     /translation="MPVRKNSMLLIAAMLATLALGTAAQRADAQVYYYAPAPVYVAPA
                     PVVYTAPVYVAPAPVVYTAPVYYYPGYCYRPSGFGFSFGYWGGHRHCYHGRGFGFSFG
                     YRGCR"
     gene            1317..1553
                     /locus_tag="P5316_12695"
     CDS             1317..1553
                     /locus_tag="P5316_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85859.1"
                     /translation="MSVQDTIREMLRREPFESFRLVTSSGQSIVVHNPDLLALLKSEV
                     FIAQPNSDKRTFVPLLHVTAVETVHNGRRSSRRK"
     gene            1817..3094
                     /locus_tag="P5316_12700"
     CDS             1817..3094
                     /locus_tag="P5316_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85860.1"
                     /translation="MRRRELLKRSLGSALAGGGMAGWLSARPARAETATAPTSRPAFP
                     EAKAVTRGPKHHFFGYYDKSPWDATGRYLLAMEIDFIGRDPQKGESLTVGMVDLQREN
                     RFIPLDVTQAWGWQQGTMLRWLGSAPDREVIYNSFDGNRYVSIIHDVHSGKTRTLPRP
                     VYDVSLDGRQAVTLDFERVNRLRPGYGYVALPERTEHDAAPKDAGIYWMDLVTGGNKQ
                     ILGLEWAAHNERLPEFEGAHHWFNHLKLNPSGTRLLFLHRWRPLSTMRFSTRMYTART
                     DGSDVRLLSQSTMFSHFDWRDDETILAWTSTKEQGNHFYLIDDRTGRHEVFAGGVLTR
                     DGHCSYSPDRKWVLVDTYPDKERRQHLMLYRPADNRLIEIGAYPANPALSGASRCDLH
                     PRWNRDGTQVCFDGSHEPTRQMYVVDVGHITQA"
     gene            3422..3853
                     /locus_tag="P5316_12705"
     CDS             3422..3853
                     /locus_tag="P5316_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRR85861.1"
                     /translation="MNDPADGVRHGVVAVIERDGRWLMIRRGEGVIAPGYWCFPGGGI
                     EAGETPELALVREIREEIGMDVRPERVIFRWQRPDGKLMLEWWRASFSDERQEPRLSP
                     DEVAEIRWVTLAELKTLRPALESNHIFLEHFEKVHKRRSRG"
     gene            complement(4118..4702)
                     /locus_tag="P5316_12710"
     CDS             complement(4118..4702)
                     /locus_tag="P5316_12710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR85862.1"
                     /translation="MAAVVFDLDGTLTVPYFDFDAIRREIGLPTQPRTPILEAMETMT
                     PEQRKRCEAILIKHEEKAAHESQLWDDTRAVLDAIRRAGIPLGVLTRNSRRSVDIVLA
                     KHGISFDCIHTREDGPIKPSPEPVLSICRKLAVDPAATWVVGDYLFDLMSGNAAGATT
                     VLMDGNGQSPDFADQADHVIHRLSELLPLLSLDS"
     gene            5071..7281
                     /locus_tag="P5316_12715"
     CDS             5071..7281
                     /locus_tag="P5316_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRR85863.1"
                     /translation="MAVTNGDLMLRTMVAAFAAGIMLIVVSRRFQLPAIVLLLLGGYA
                     CGPQILGIVRPDSLGGGLVVIVELAVGLILFDGGLTLDVQGYRLASGPIRRLLTVGVV
                     VTWLGTAVAIGVIFPDLPMSMALLAGSLVIVTGPTVIGPLLKRTKVNARLNHILHWES
                     VLIDPIGVFVAILCFEWISGMEGGTAIAHFGMRVFWGLLVGIGGGLLVHRLLHGGFVP
                     EELANISVLAMAVLIFGLGEVVLSKSGLLSVTSAGFVIGLKNPPELKQIRRFKAEIAD
                     LAIGILFILLAARLRSEQFLAFGAGGALLVAIVMFIIRPLNVVVSTVGYNVPWKEQLF
                     LSWIAPRGIVAASMASLVSLQLADAFPGSAAFVETFVYSVIVGTVVLQGLTAGLVADV
                     LKVRAPAPTGWMIVGAHRLGQEVARFVSETARARAILVDTNAKMVSEARQEGLQAIVA
                     DARDLDLIERDEFQGIGNLVALTDNEDLNMLLCQRWSEAFGRSRVFFWSPSADRRPVE
                     RAGQAVWSRLPKPSILSAELARNEAAAVLDTSSDPGRLSQGTPIAAFYKNQVNLTPAD
                     SSPAVATESYPLLVLRREADYLRRGLRPELAVRLEISEQKALFEDLVSRIVEVEPKVP
                     RDAMLKELIDREQVWPTALGHGIAVPHAYCRALTSRICAVAQVPGGVDFKAPDGEKVR
                     LVFLLLSPQGDPEGHLATMAEIARLVGDPEVRDSLLSAQGPEDLLARLRTAAPA"
     gene            7296..8426
                     /locus_tag="P5316_12720"
     CDS             7296..8426
                     /locus_tag="P5316_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRR85864.1"
                     /translation="MTAKGGKRVTVGAVAAAMDRIAPPALAQSWDNVGLLVGDRRAVC
                     RRVLLCIDLTPAVLREAVASRCEMIMSYHPPLFKPVKRIVADSDGTEAIVHEAIRRGI
                     AIYSPHTALDAAEGGVNDVLAGLCGLTDVGPFEYVSVGRREYKVVTFVPFAQLEEVAA
                     AMFAAGAGRIGDYEQCSYRLRGEGTFFGTESTNPQVGRKGRLERVAETRIEMIAPERC
                     LPEVIAALRHAHPYEEPAFDIYPLTPEPTGGIGRVGTLPKGTTLAGLAKSLKRATRSR
                     VLMTVGERNTKLRRAAVCGGSAGRMPLEMPRASACDVIVTGEIRHHDALSILRAGKTA
                     IALGHWESERPVLAPLGKRLKKLVGSIDIRMSRKDAPPFQTA"
     gene            complement(8901..11513)
                     /locus_tag="P5316_12725"
     CDS             complement(8901..11513)
                     /locus_tag="P5316_12725"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HRR85865.1"
                     /translation="MVSRTCRWMLAITLLTAAPVRPAEEPTTASAPTSQGIPAISGSA
                     LSDLLPAKTLLYKGWPGIDVFSKAAEETELAKLLKEPELERLRASWWKDIWPAIKDQI
                     ARAIGDEKAEQILDPMTTLLGTVWRHPTAVALIGAGTGKNGPQFDAAIIVRAGKDSAK
                     LVEIAEQLLALAGITPDSAMEVEVGRLKFKTVTGFPLPLRWGRAQDLFVVSLGTKATE
                     HLGLGTLEDSLTRSPRFAAAMKHVGADQSWPFFFLDMKAAVQALKDFQPMLAATSLPF
                     FAEPDALDRFLKQMGVEACESLSIAIAPEAGGFKTSMFVHLPGAAASSNPLIGRKALI
                     NDDIRVVPKNVRWATVTNWDAAATYRSLLDALTSLVPTGEEEILGMVERAERRLGLSI
                     EKEILGAFEDNWAIFDSPEFGGIWVSGITVVAKVKPKHHLRKAMRNITAVIAEEMSGD
                     ASAEVATETYRGQTIEYFNISGVPLPIAPAWAEFQGRWIMALYPQMVRMELDHLMNRG
                     ASLLENPDYQRGRKLLPNGAFAVNYVDTKSGMLQMYSLALPIWQAAAAMLQKENIPLD
                     VSMLPSAHTVTRHLFGNVSVSIVSEDGALTVSHGALPVPVPAIGEGGFAIPLGAAILL
                     PSLSRARVLAKRTASAANLSGLGKALYTYAVEHDDQFPPDLRTLVDSGAISEKSLRSP
                     LDESDATSSYIYISGQTTEVDQRNVIAYENPENHDNEGTVVLFADSHVEFMTMSAFRK
                     ALEDTYRRLGREMEEPGEQVEARTRDQKTAAARRAVSREGALAAALELFKMHVGRYPN
                     KLDELVEQPESEADAEKWRGPYVQGAGALKDPWGHRLMYLSPGERNRSGYDLWSLGPD
                     GQDGTTDDLANWKK"
     gene            complement(11562..11978)
                     /locus_tag="P5316_12730"
     CDS             complement(11562..11978)
                     /locus_tag="P5316_12730"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRR85866.1"
                     /translation="MGWRRKKTPGLSREMLLAARPLRNPNITEKEIAGGGLELTVSLP
                     RSRLFRWLSGGCNHPIVRRYQLDVIGVETWRMIDGRTTIMTMIERLGSAHGMEPRQAE
                     AAMLAYLRTLAQRGIMMLALPHEEPRAADKDRLHTS"
     gene            complement(11985..12995)
                     /locus_tag="P5316_12735"
     CDS             complement(11985..12995)
                     /locus_tag="P5316_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRR85867.1"
                     /translation="MILAHDRPDGDALGAMAAMKRVIEAAGREATAFVYDELPGRYAF
                     LDGIPAQSRGHGIGINTKQVFHRWQDASPGAIDARFDGILILDTNSWSQIKPAADYLR
                     ASALPRIVVDHHAAADGVSQGHGSGLDLIDPSAPATCQILHAWCDAMDWPLDSASAEA
                     LFTGLSTDTGWFRHSNTDARAMQTAADLIDLGVRPDVLYGRLYASFPLSRVRLMTEML
                     ATLTFHADNAIAIAHITPEMFARSGASKAETEDLVNEPMAAEPVIVSALLVDAGTGEI
                     RASFRSKSPEVCGRDIDVAAIAGRFGGGGHRRAAGARLAGTLEEARRRVTEKILAAMR
                     GE"
     gene            13269..13751
                     /locus_tag="P5316_12740"
     CDS             13269..13751
                     /locus_tag="P5316_12740"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRR85868.1"
                     /translation="MQGPYAVLFLCTGNSCRSQMAEALLRHLGGERFQALSAGSHPAG
                     YVHDLALEAMRRMGVSTAGLYSKSWDEFAVTPIDIVLTLCDHAAGQSCPVFPGPAVRA
                     HWSLPDPAFAQGTDEQRLMFAMQVASRLRGWIEKLIRLPIEKLSPQQLRAELERIPRF
                     "
     gene            14044..15405
                     /locus_tag="P5316_12745"
     CDS             14044..15405
                     /locus_tag="P5316_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRR85869.1"
                     /translation="MANGTLPAYLASAKPNPMSNRGPWWTNTAPAYFGIFMWIAFYDK
                     LASAFNLGGLDVALGGLVIAAVLSHYVFHYVFGVLGMKTGYPLYVVGSSTFGTVGGLL
                     FPGIFMGLLQIGWYSVGTFYAAKLLLLAIGRDADQISALFIVFCIAWGYVFALFGAFG
                     IKYVAKISSYLPWVAVAMLCIAGVCALPHVGNFKFAVDPTGPGVLAGACLVIQMVIGF
                     FATAGAAGVDFGTNSRDENDVNLAGLTGISLSILLAGGLALIIVAGAKGIDPTVGPQV
                     SKALPLVSPGLAKIMFLLLAIGSMAPACFCASIIGNSLSTMIPTMGRVPLTLGGATIG
                     IILAATGIAGNLEAFFGLIGASFGPICGAIVADYFLSGKKWAGPRQGVSLAGYAAWVL
                     GFLVGISSNDMVTKLLGRELVPGWHPTAVYSFIVGFVVYAILAKAGLQGRTVAYPGAD
                     ASK"
BASE COUNT         2677 a         4767 c         5146 g         3128 t
ORIGIN      
        1 gggccgcaac ccaagcgttc tgcgatccag aaacatgcgc gcaagccgca cagatgtctg
       61 gccgtaaggt tctaatgggg ccgaggaccg ccgtaagcag atccgcgggt ggattcgcct
      121 gagagacaac atgccaagtt ttcaccaagc ccagcaacta caagaatttg cgcaagaaat
      181 gtttgagatg tcgaaggaca tctgggcggc ccagaacaag tcaaagacaa aggatcagac
      241 ggagttgacg gagacggagt tcctcacact ggatctcttg gccgaccggc aaccgcaaac
      301 cgtgggcgat cttcagaaac agatcggcat actccccacc cagatgtccc gagtcatccg
      361 tgcgctggag agtaagagcg acgggccact gatcaggtgc cagattaacc agcaagacaa
      421 gcggaaagtg gacgtggaac tgaccgaggc gggccgccaa gcccaccaag cgtaccggca
      481 attcaagctg ggtagcatcc agcgcttgct cacgggtttg agcgagcagg accgcttaga
      541 acttgtgcgg attctccgtc tggtgcggca agaagcccgt aaagtcttgt cccaaaagta
      601 gttgtgcctc atttcggtcg cctgcccaag aatcctcaca aaatagtgcg cttccccaaa
      661 ctttttcggg gtcaacagtg tctaaccaac tgaccggttc gagaggtttc ggagacgacc
      721 gtgaaggcgg aggtcttgag gaaaccgtag accaaggagg tttgccatgc cagtcaggaa
      781 gaattcgatg ttgctgattg cggcaatgct cgcgacgctg gccctcggga cggcggctca
      841 acgggccgat gctcaggttt attactacgc accggcgccg gtctatgtgg ccccagcacc
      901 agtggtctac accgccccgg tgtatgtagc ccccgcgccg gtcgtgtaca ccgctccggt
      961 ttattactat ccgggttact gctatcgacc gtcggggttt ggcttctcat tcgggtattg
     1021 gggtggacac cgtcactgtt accatggccg aggtttcggc ttcagctttg gctatcgcgg
     1081 ctgccgctga ttgacggcgg cccacactta ggggtttaac gtaggcggtg gagtctacgc
     1141 ggcaccaggc cgggtgtgtt gcccggcctg gtattttttg cgccgcaaga aaagaggagg
     1201 gccgccgctg tggcgccgct gttcatctct cgccaagcgg caaagctctc ttcttgcggg
     1261 ctgttcaccc ctcggtcagc agctcgaccc gccgcccgcg cggttataat gctggtatga
     1321 gcgtacagga taccatccgc gagatgctcc gccgtgagcc ttttgagtct tttcggctgg
     1381 tcacgtccag cgggcagtct atcgtggtcc acaacccgga ccttctggcc ttgctcaagt
     1441 cggaggtgtt catcgctcag cccaattcgg acaaacgcac cttcgtcccc ctgctccatg
     1501 tgactgccgt tgagaccgtg cacaacggac gcaggagttc ccgccgaaag tagcaggaac
     1561 tcgcggtaca agaggcggaa ggtggatacg ctgaatatgc gaaaggtcgt cctggactgg
     1621 ttccgcgcag gccgcgtgtt ccgtggtttg atgagaacga cgccggcagg ggaagggcct
     1681 ttgttttttg tagaagtaga tgttcttttt cctcagtatc tgatcgtgag cagggcgcgg
     1741 gcgggggata gctgctttcg gtgcggcaca tgcagacgag gacggcgtgc tgagcacgcc
     1801 ttaccgagga gaaaccatga ggcgacgcga attgctcaaa cgatcgttgg gctcggcgct
     1861 ggccggcggt gggatggcgg gttggctctc ggctcggccg gcaagagcgg aaaccgcgac
     1921 tgcacctacg agcaggccgg catttccgga ggcgaaggcg gtcacccgcg ggccgaagca
     1981 tcatttcttc ggctactacg acaagagccc gtgggacgcg accggtcgat acctgctggc
     2041 gatggagatc gacttcatcg gccgcgatcc gcagaagggc gagtcgctga ccgtcggcat
     2101 ggtcgacctt cagcgcgaga accggttcat tccgctcgac gtgacgcagg cctggggctg
     2161 gcagcagggc acgatgctgc ggtggctggg ttcggccccg gatcgcgagg tcatctacaa
     2221 cagcttcgac ggcaatcgct acgtttcgat catccacgac gtgcacagcg gcaagacgcg
     2281 cacgcttccg cggccggtgt acgacgtcag cctggacggc cgtcaggcgg tgacgctcga
     2341 tttcgagcgg gtcaaccggt tgcggcccgg gtacgggtac gttgcgctgc ctgaacggac
     2401 cgagcatgac gcggccccca aggacgcggg catctactgg atggacctcg tcactggcgg
     2461 caacaagcag atcctcgggt tggagtgggc ggcacataac gagcggctgc ctgagttcga
     2521 aggtgctcac cactggttca accacctgaa gctcaacccg tcgggcacgc ggctgctgtt
     2581 cctgcaccgc tggcggccgt tgtcgaccat gcggttcagc acccggatgt acaccgcccg
     2641 caccgacgga agcgacgtgc ggctgctctc gcagagcacc atgttctcgc atttcgattg
     2701 gcgcgacgac gagacgatcc tggcctggac gagcaccaag gaacagggga atcactttta
     2761 cctgatcgac gaccgcacag gtcgacacga ggtcttcgcc ggcggcgtgc tgacgcggga
     2821 cggccactgc tcctattcgc cggaccgcaa atgggtgctc gtcgacacct atcccgacaa
     2881 agagcgtcgg cagcacctga tgctctatcg gcccgcggac aaccggctga tcgaaatcgg
     2941 cgcttacccg gccaatcccg cgctgtcggg ggcgtcccgc tgcgacctgc acccgcgctg
     3001 gaaccgcgac gggacgcagg tctgcttcga cggcagccac gaaccgacac gccagatgta
     3061 tgtggtcgac gtgggacaca tcacgcaggc gtgagtgtgg tggccgaggg cgagcgggtg
     3121 ccccatctgc ttcttgcaga tgggggagcg atgattgtcg gtcaaaggca cccctcccga
     3181 ctgtcgctcg gaccggaatc tgttctgggc cgccttccct cgcagcatcc gtttcgttct
     3241 cggcgacaac gagcatggta atccttgttg aagaggcccc ggatgggaag ctcgagttgg
     3301 ggcgggcgtt ctcggttttg ccggtcgatc gtcgctcccc cacctcgcgg acgaggtggg
     3361 gcacccttgg gggtgcgcgg atgctttgtg cgcggtggtc aagcgtctat gatgggctgc
     3421 tatgaatgat cccgcggatg gtgttcggca cggcgtggtg gcggtcatcg agcgtgacgg
     3481 ccggtggttg atgattcggc gcggcgaggg ggtcattgcg ccggggtact ggtgttttcc
     3541 aggcgggggc atcgaggcgg gcgagacgcc cgagttggcg ctcgttcgcg agattcgcga
     3601 ggagatcggg atggacgtgc ggccggagcg ggtgatcttc aggtggcagc gtccggacgg
     3661 caagctgatg ctggaatggt ggcgggcgtc gttctccgac gagcggcagg agccacgact
     3721 gtcaccggat gaggtggccg agatacgatg ggtcacgctc gcggaattga agactctgcg
     3781 gccggcgctg gagagcaatc acatcttcct cgagcatttc gagaaggtac acaaaagacg
     3841 atcgagggga taaacccctc ggctcgtcaa gcgtatccct ccggtttcgc ctgcacgctg
     3901 cccgaagacc gagggggtaa acccctcggc tcgtcaagcg tatcccgtcg gtcttacccc
     3961 tccggtttcg cctgcacgct gcccgaatac cgaggggata aacccctcgg ctcgtcaagc
     4021 gtatcccgtc ggtcttaccc ctccggtttc gcctgcacgc tgcccgaata ccgaggggat
     4081 aaacccctcg gctcgtcaag agaatccggc tcggcgatta cgaatccaac gacagcagcg
     4141 gcagcaattc gctcagccgg tggatgacgt ggtcggcctg gtcggcgaaa tcgggcgatt
     4201 ggccgttgcc gtccatcagc acggtggtcg cgcccgcggc gttgcccgac atcaggtcga
     4261 acagatagtc gccgaccacc caggttgcgg ccgggtcgac cgccagcttt cggcagatgg
     4321 atagaacagg ctccggcgag ggcttgatcg gcccgtcctc gcgagtatgg atgcagtcga
     4381 agctgatgcc gtgcttggcc agcacgatat ccaccgatcg gcggctgttg cgggtcagca
     4441 cgccgagcgg aatgcccgca cggcggatcg catcgagcac cgcccgcgtg tcgtcccaga
     4501 gctggctttc gtgggcggcc ttctcctcat gcttgatgag gatagcctcg cagcgtttgc
     4561 gctgctcggg ggtcatggtc tccatcgcct cgaggatggg cgtgcgaggt tgggtcggca
     4621 ggccgatttc ccgccggatc gcgtcgaagt cgaaatacgg cacggtgagc gtgccgtcga
     4681 ggtcgaagac gacggcggcc acgcgagcat cggtggtgtt ctcgggtttg gatgccttca
     4741 tacaggtggt ttagctggcc aaccggcagg ggtcaagcgg ggaggccttg gaccgcaggt
     4801 gcatcttctg gtcaggaaga atgggtccgt gacagattag gcaggcaggc tctgtgggtg
     4861 ccactggcgg tttatcgcga gccgcgtcga gcggcttgcc cgccagtgca tgcctgagaa
     4921 gcaaaagaca ctggcagaca agctgccagt ggcacccgcc taaagtgtca cggacccagg
     4981 agcaatcccc ctgaggaaat ggtcttggct ggaccgccga ttgcagttat ctttcgctcg
     5041 gaaatcgatt gacggcggcg ggacacagac atggctgtta ccaatggcga cctgatgctt
     5101 cggaccatgg ttgccgcctt cgcggccggc atcatgctga tcgtcgtttc gcgccgattt
     5161 cagttgcccg cgattgttct gctgctgttg ggcggatacg cgtgcggccc gcagatactg
     5221 ggcatcgtac ggccggactc gctcggaggc ggtctggtcg tcattgtcga gctggccgtg
     5281 gggctgatcc tgttcgacgg cggcctgacg ctggacgttc aaggttaccg gctggcctcc
     5341 gggcccattc ggcgtctgct gacggttggc gtcgttgtca cttggctggg aacggcggtg
     5401 gccatcggcg ttatcttccc ggacctgccc atgtcgatgg ccctcctggc gggcagcctg
     5461 gtgatcgtca ccggtccgac ggtgatcggg ccgctgctca agcgaaccaa ggtcaacgcc
     5521 cggctcaacc atatcctcca ttgggagagc gtgctgatcg atccgatcgg cgtcttcgtg
     5581 gcgattctgt gctttgagtg gatcagtgga atggagggtg gtaccgccat cgcacatttc
     5641 ggcatgcgcg tgttctgggg gctgctcgtg gggattggcg gcggcctgct ggtacaccgg
     5701 ctgcttcatg gcgggtttgt ccctgaggag ttggccaaca tctcggtgct ggcgatggcc
     5761 gttctgatct tcggcctggg cgaggtggtc ctgtccaaat cgggcctgct ctccgtgacg
     5821 tcggccgggt ttgtgatcgg gctcaagaac ccgcccgaat tgaagcagat ccgccggttc
     5881 aaggccgaga tcgcggacct ggccatcggg atcctgttca tccttctggc cgcgcggctc
     5941 aggagcgagc agttcctcgc cttcggggcc ggcggcgcgc tgctggtggc gatcgtcatg
     6001 ttcattattc gtccgctgaa tgtggtggtt tcgaccgtcg gctacaacgt gccgtggaaa
     6061 gagcagcttt tcttgtcatg gatcgcccca aggggcattg tcgcggcgtc aatggcgtcg
     6121 ctcgtctccc tgcagcttgc cgacgcattc ccgggatcag cagcgttcgt cgagacattt
     6181 gtttactcgg tgattgtagg cacggtcgtt ctgcaaggac tcaccgccgg gctggtggct
     6241 gacgtgctca aggtgagggc tcccgccccg accggctgga tgatcgtcgg ggcgcatcgg
     6301 ctgggtcagg aggtggctcg ttttgtcagc gaaacagcac gtgcgcgcgc gatcctggta
     6361 gacaccaacg ccaagatggt ctccgaggcc cggcaagagg gcctgcaggc gatcgtcgcg
     6421 gacgctcgcg atctggatct catcgagcgg gacgagtttc aggggatcgg caatttggtg
     6481 gccctcaccg acaacgaaga cctcaacatg ctgctctgcc agcggtggtc agaagcgttc
     6541 gggcggtctc gcgtcttctt ctggtcgccc agcgcggatc gtcgcccggt cgagcgggcg
     6601 ggacaggcgg tgtggtcgcg attgcccaag ccgtccatcc tgagcgcgga gctggcccgc
     6661 aacgaggccg cggcggtgtt ggacacgtcc tcggaccccg gccgtctttc gcaaggaacg
     6721 cccatcgcgg cgttctacaa gaaccaggta aacctgacgc cggcggattc ttcacccgcg
     6781 gtcgccacgg agtcctatcc gctgcttgtg ctgcggcgcg aggcggatta tctgcgcagg
     6841 gggctgcggc ccgagctggc ggttcgattg gagatctcgg agcagaaggc gctgttcgag
     6901 gatttggttt cgcgaattgt cgaggtcgag ccgaaggtac ctcgcgacgc catgttgaag
     6961 gaactgatcg accgcgagca ggtctggccg acggctctgg ggcacggcat tgccgttccg
     7021 cacgcctatt gccgggcgct gaccagtcgc atctgcgcgg tggcccaggt tcccgggggc
     7081 gtcgatttca aggcgccgga cggcgagaag gtgcgcttgg tctttctgtt gctcagtccg
     7141 cagggtgatc ctgaagggca cctggccacg atggccgaga tcgctcgctt ggtaggcgac
     7201 ccggaggtga gagactcact gctgtcggcc cagggccccg aggatctgct ggcaaggctg
     7261 cgaacggcgg caccggcgtg aagagaggat cgcagatgac ggcaaaaggc ggcaagcgcg
     7321 tgaccgtggg agccgtggct gcggcgatgg atcggatcgc accacccgcg ctggctcagt
     7381 cgtgggacaa cgtcgggctg ctggttggtg atcggagagc ggtgtgcaga cgcgtgctgc
     7441 tgtgcatcga cctgacgccg gcggtgctgc gcgaggcggt cgcgtcgcgg tgcgagatga
     7501 tcatgagtta tcacccgccg ctgttcaagc cggtcaaacg aattgtggcg gacagcgatg
     7561 ggacggaagc gatcgtacac gaggcaatta gacgcgggat tgcgatctac tcgccgcaca
     7621 cggctctcga tgccgccgag ggcggagtca acgatgtgct cgcgggtctg tgtgggctga
     7681 cggatgtcgg gccgttcgag tatgtgtccg tcgggcggcg cgagtacaag gtggtgacgt
     7741 ttgtgccttt cgcccaactc gaagaagtgg ccgcggccat gttcgcagcg ggcgcgggtc
     7801 gcattggcga ttacgagcag tgcagctacc gcctgcgcgg cgagggcaca ttcttcggga
     7861 ccgaaagcac caacccgcag gtcggccgca agggccggct tgagcgcgtg gccgagacgc
     7921 gaattgagat gatcgcgccg gagcgctgtc tgcccgaagt gatcgcggca ttgcgacatg
     7981 cacatcctta cgaggagccg gcctttgaca tctacccgct gacgcccgag ccgacgggcg
     8041 ggatcggccg tgtcggcact ctgccgaagg gcactacgtt ggccggcttg gcgaaatcac
     8101 tgaagcgcgc gacgaggtcg cgagtgctga tgaccgtcgg cgagcgaaat acgaagctgc
     8161 gccgcgcggc cgtctgcggc ggctcggccg ggcgcatgcc gctggagatg ccgcgggcgt
     8221 ccgcctgcga cgtcatcgtc accggcgaga tccgccacca cgacgccctg tcgatcctcc
     8281 gcgcgggcaa gacggccatc gccctgggcc actgggaaag cgaacgcccg gtcctggccc
     8341 cgctgggcaa gcgattgaag aagctggttg gctcgatcga tattcgcatg agtcgcaagg
     8401 atgcgccgcc gttccagacc gcctgacgag cgcgtcgggt ccgtcctcgc ggacctcgat
     8461 ctgcaatctc aaatctgcga tttgaaatcc gatggtccgc tggcgcgaga aacccttcaa
     8521 tcgctggtgc gagaatccct tctcgcacct cttccgcagc gaatcttttc gccatttctc
     8581 atggcgggat gggaatcccg cgtttgtggg gccgagatag gaatctcggc ccagcggtct
     8641 gcaatctgcg ctggcgagta tcctttctcg cacctcttgc gcggcgaatc ctttcgtcat
     8701 ttctcatcgc gggatgggaa tcccgtgttc tatggggccg agataggaat ctcggcccag
     8761 cgatgctcat cgcgggatgg gaatcccgtg ttctatgggg ccgagatagg aatctcggcc
     8821 cagcgggttt gtgggagcgg gatgggaatc ttggcccggc ggcgtggtcc gcccgggcgg
     8881 agcctgccgg gctgccgaga tcatttcttc cagttggcga gatcgtcggt cgtgccgtcc
     8941 tggccgtcgg ggccgaggct ccagagatcg taaccgctgc ggttgcgttc gcccggcgag
     9001 aggtacatca agcgatggcc ccacggatcc ttcagtgcgc cagcgccttg gacgtacggc
     9061 cccctccact tctcggcgtc ggcttcgctt tcgggttgtt cgaccaactc atccagcttg
     9121 ttgggatatc gcccgacgtg catcttgaac agttccaggg ctgcggccag ggctccctcg
     9181 cgactgaccg cccggcgtgc ggccgccgtt ttctggtccc gtgtcctcgc ctctacctgt
     9241 tcaccgggtt cttccatctc gcgtccaagc cgacggtatg tgtcctcgag ggccttgcgg
     9301 aaagcgctca tcgtcatgaa ctcgacatgg ctgtcggcga aaaggacgac ggtaccttcg
     9361 ttatcgtggt tttcggggtt ctcataggca atgacgttgc gttggtcaac ctccgtcgtc
     9421 tggccggaga tgtagatgta cgaactggtt gcgtcggatt cgtcgagcgg cgatcggagt
     9481 gatttctcac tgatggcgcc gctgtcgacc agtgtccgga gatcgggcgg gaactgatcg
     9541 tcgtgttcga ctgcataggt atacagagcc ttgcccaggc cactgagatt ggccgcgctg
     9601 gccgtgcgct tggccagtac gcgggccctg gacagagacg gcaacaggat tgccgctccc
     9661 agcggaatcg caaaaccccc ttcaccgatg gccggcaccg gaaccggcag ggccccgtgc
     9721 gagaccgtca acgccccgtc ctcgctgact atcgagacgg acacgttgcc gaacaggtga
     9781 cgggtgacgg tatgggccga cggcaacatg ctgacatcga gcgggatgtt ctccttttgc
     9841 agcatggcgg cggcggcctg ccagatcggc agtgcgaggg agtacatctg gagcatgccc
     9901 gacttggtgt ccacgtagtt gaccgcaaac gctccgttgg gcagcagctt gcgcccgcgc
     9961 tgatagtcgg ggttctcgag cagcgaggcg ccgcgattca tcaggtggtc cagctccatc
    10021 cgcaccatct ggggatagag agccatgatc caccggccct ggaactccgc ccaggccggg
    10081 gcgatcggaa gcggcacgcc gctgatattg aagtattcaa tggtctgccc acggtatgtt
    10141 tcggtcgcca cctctgccga ggcgtcgccg ctcatctcct cggcgatgac ggccgtgatg
    10201 ttgcgcatcg ccttgcgcag gtggtgcttg ggcttgacct tggccaccac cgtaatgccg
    10261 ctgacccaga tcccgccgaa ctcgggcgaa tcgaagatcg cccagttgtc ctcgaacgcg
    10321 ccgagaatct ccttttcgat gctcagtccg aggcgtctct cggcccgttc caccatcccg
    10381 aggatctctt cttcgccggt cgggacaagc gacgtcagag catccaacag tgagcgatag
    10441 gttgcggctg cgtcccagtt ggtcacggtg gcccagcgga cgttcttggg gaccacccgg
    10501 atatcgtcgt tgatcaaggc ttttcggccg atgagcggat tactgctggc ggcggccccg
    10561 ggcaggtgaa cgaacatgct ggtcttgaag ccgccggcct cgggcgcgat cgcaatggag
    10621 agcgattcgc acgcctcgac gcccatctgc ttgaggaagc gatccagagc gtccggctca
    10681 gcaaaaaacg gcaaactggt ggctgcgagc atcggctgaa agtccttgag cgcctggacg
    10741 gccgccttca tgtcgaggaa gaaaaacggc caggactggt cggcgcccac gtgcttcatt
    10801 gccgcagcga aacgcggact gcgggtcagc gaatcctcga gcgtgccgag gccgaggtgc
    10861 tccgtggcct tggtgccgag ggagacgacg aacaagtcct gcgccctgcc ccaccgcagg
    10921 ggaagcggaa aaccggtcac ggtcttgaac ttcagcctgc cgacctcaac ttccatcgct
    10981 gaatcgggag ttatcccggc cagagccaga agctgctccg cgatttcgac gagtttcgcc
    11041 gaatccttgc ctgcacggac gatgatggcg gcgtcgaact gcggtccgtt cttgccggtg
    11101 ccggccccga tcagagccac cgccgtcgga tgccgccaga ccgtgcctag cagcgtcgtc
    11161 attggatcga gaatctgttc ggccttctcg tcgccgatcg cacgggcgat ctgatctttg
    11221 atggccggcc agatgtcctt ccaccacgag gctcgcagcc gttcgagttc gggttccttc
    11281 agcagcttgg ccaactcggt ctcctcggcc gccttcgaaa agacgtcgat tccaggccac
    11341 cccttgtaca gcagcgtttt ggccggcagc agatccgaga gcgccgagcc gctgatcgcc
    11401 ggaatgccct gcgaggtcgg cgccgatgca gtggtcggct cttcggccgg tcggacgggt
    11461 gctgcggtta gcaacgtgat cgccagcatc cagcgacatg tcctggaaac catgattgat
    11521 tctcctttcc gcggattgtc cttccgcccc gcaagatcgg ttcagctcgt gtgaaggcga
    11581 tccttgtcgg ctgctcgggg ttcttcatgc ggcagcgcca gcatcataat gcccctttgc
    11641 gccagagtcc gcaagtatgc gagcatcgcc gcctccgcct gtcgcggctc catgccgtga
    11701 gcagagccga ggcgttcgat cattgtcatg atcgtcgtcc ggccgtcgat cattctccag
    11761 gtttcgacgc cgatcacgtc gagttggtac cggcggacga tcgggtggtt gcagcctccg
    11821 ctcagccagc gaaacaggcg gctcctgggc agcgagacgg tcaattcgag gccgcccccg
    11881 gcgatttcct tctcggtgat gttcgggttt cgcagcggcc gggcggccag aagcatctcc
    11941 ctgctgagtc ccggggtttt ctttcgtcgc cagcccattg tcggtcattc tccgcgcatg
    12001 gccgcgagga tcttctccgt cacgcgccgc cgggcttctt ccagggtacc ggccagtcgc
    12061 gctccggccg cccggcggtg cccgcctccg ccgaaacgtc cggcgattgc cgccacgtcg
    12121 atgtcacggc cgcacacctc gggcgacttg ctgcgaaaac tggctcgtat ttcccctgtt
    12181 cccgcatcga ccaggagcgc cgagacgatg accggctcgg cggccatcgg ctcgttgacc
    12241 agatcctccg tctcagcctt tgacgctccg gaacgggcaa acatttcagg ggtgatatgc
    12301 gcaatcgcaa tcgcgttgtc agcgtggaac gtgagcgtgg ccagcatctc ggtcatgagt
    12361 cggacgcgcg acaggggaaa cgatgcgtac agcctgccat agaggacgtc aggcctcacg
    12421 cccaggtcga tcaggtcggc tgcggtctgc atggcgcggg cgtcggtatt cgagtgccgg
    12481 aaccatccgg tgtcggttga caggccggtg aacaaggctt ctgccgaggc ggaatcgagc
    12541 ggccagtcca ttgcatcgca ccaggcgtgc agaatctggc acgtggcagg cgcggaagga
    12601 tcaatgagat caagaccgga tccgtgacct tgggaaacgc cgtcagcggc ggcgtggtga
    12661 tcgacgacga tcctgggcag ggcgctggct cgcaggtagt cggcggccgg tttgatctgc
    12721 gaccagctat tggtgtcgag gatcaggatc ccgtcgaagc gcgcgtcaat cgcgccagga
    12781 ctggcgtctt gccagcggtg gaagacctgc ttcgtgttga tcccgatgcc atgcccacgg
    12841 ctttgcgcgg ggatgccatc gaggaacgca taccggccgg gcaactcgtc gtaaacgaac
    12901 gcagtcgcct cacggccggc ggcttcgatc acccgtttca tggccgccat cgccccgaga
    12961 gcgtcgccat ccggccggtc gtgggccagg atcatcgtcc gcttccatcc ggccagaagc
    13021 cgtccggccc cttccaatgt ttctgggtcg accatcactc acctgtaagc ggtaaccgca
    13081 atcatcattc tcgttgtacc ggggggacca tgcgggcagc cttccccgtt cttgtcttct
    13141 cgcgaggaca gggccggccc gcgcatcaat cgacgcacgc ttgccatttc cggcgacacc
    13201 tcacttgaga gataatacca cggttggcgt tttgggctca gtccgtcgcg cataaagggg
    13261 tctctccgat gcagggacca tacgccgtgc tgtttctttg taccggcaat agctgtcggt
    13321 cccagatggc agaggctttg ctgcgtcatc tgggcgggga gaggttccaa gcgctcagtg
    13381 ctggcagcca ccccgcaggc tatgtccacg acctggcgct cgaggccatg cggcggatgg
    13441 gcgtgtctac tgccggcctc tattccaaga gctgggacga gtttgcggta acccccatcg
    13501 acattgttct aacgctctgc gaccatgccg ccggtcagtc ctgtccagtc ttccccggtc
    13561 cggcggtccg ggcgcactgg tctttgcccg accccgcctt tgcccaaggc accgacgaac
    13621 agcgcttgat gttcgccatg caggtcgcaa gccggctccg gggctggatc gagaagctta
    13681 tccgcctccc gatcgagaag ctctcgcctc agcagttgcg ggccgagctt gaacggatcc
    13741 cgaggttctg acggtttcgc gtcaccctcc gccatccttc ggaggttctg cccccccgcg
    13801 aagtagcgca agacgcacaa acagggcaga gcaggattgg agtcctgcga agatccatgg
    13861 aggaggtggg gcggaaacct gccggtcccg gctgatgctc ggaaccctgt cgggcgcatc
    13921 gaacggcacc ctggcaacaa gcagaaaaac ggttaccatg tcccacgacc gatcaaatcg
    13981 gccggaggca agcggtagcc ctcacggtcg gacatgccgt cctagagttg aaggagaaca
    14041 cctatggcga acggaacact tccagcttat ttggcatcgg ccaagccgaa ccccatgagc
    14101 aaccgcgggc cgtggtggac caataccgcc ccggcgtact ttggcatctt catgtggatt
    14161 gccttttacg acaagctcgc ctcggctttt aaccttggtg gtctggatgt tgctctgggc
    14221 gggttggtaa tcgccgccgt gctcagccac tacgtgtttc actacgtatt cggcgtgctc
    14281 ggtatgaaaa cggggtatcc gctttacgtg gtgggtagct ccacattcgg cactgtgggc
    14341 ggcttgcttt tcccgggcat cttcatgggc ttgctccaga tcggctggta cagcgtgggg
    14401 accttctacg cggcaaagct attgctgctg gccatcggcc gcgacgcgga ccagatttcc
    14461 gcgctgttta tcgtcttttg catcgcgtgg ggttacgtct ttgccctctt tggtgccttc
    14521 ggaatcaagt acgttgcgaa gatatcgagc tatctgccgt gggtggccgt ggccatgctg
    14581 tgcatcgccg gcgtgtgtgc cctgccgcac gtggggaact tcaagtttgc cgtggacccg
    14641 accgggccgg gcgtgttggc cggggcttgc ctggtgattc agatggtcat cggtttcttc
    14701 gctaccgccg gggcggccgg tgtggacttc ggcaccaaca gccgggacga gaatgatgtc
    14761 aatctggccg ggctcaccgg catcagtctg tccatcctcc tggccggcgg tttggctttg
    14821 atcatcgtgg ccggcgcgaa ggggattgat cccaccgtcg gtccccaggt cagcaaggcg
    14881 ttgccgttgg tcagccccgg cctggcgaag atcatgttcc tgctgctggc gatcggctcg
    14941 atggccccgg cctgtttctg tgcgagcatc atcggcaaca gtctgtcgac catgattccg
    15001 accatggggc gcgtgcctct gaccttgggc ggggcaacca tcggcatcat cctggcggcc
    15061 acgggtatcg ccggcaatct ggaggcgttc ttcggcctca ttggcgcatc tttcggtccg
    15121 atctgtgggg ccatcgtggc cgattacttc ctctcgggta agaagtgggc cggccctcgc
    15181 cagggcgtca gcctcgccgg ctatgcggcg tgggtactcg gcttcctcgt cggcatctcc
    15241 agcaacgaca tggtgaccaa gctcctcggc agggaactcg tgcccggctg gcatccgacc
    15301 gcggtttact ctttcatcgt gggcttcgtc gtatacgcga tcctggccaa ggccggtttg
    15361 cagggcagga cggtcgccta tccgggtgcc gatgcatcca agtgacgatc ggtcgatagg
    15421 gctttcaaac aacgggcgtg gagcaccatc aaggtgcctc gcgcccggtt tctttggtca
    15481 caaccttctg cgcgcgatct cggagtccga ccacgatctg tacgtgtttg gcgtgcaaga
    15541 cagcgggtca ggcacttttt gccgtcttct tgtcgccgac gagtcccgcc ggactgagcc
    15601 aacctctccc aatcgcaccg cgaactgcat ttgggcaaaa agagccagag cccagcacgc
    15661 tagcccaagt tgaacaccaa agggcgattc tagttttttt tcggggtgtt ggaccgca
//
LOCUS       DAOTPW010000131        15587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3754_length_15587_cov_5.616405, whole genome shotgun sequence.
ACCESSION   DAOTPW010000131 DAOTPW010000000
VERSION     DAOTPW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15587
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3754_length_15587_cov_5.616405"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(503..1639)
                     /locus_tag="P5316_12750"
     CDS             complement(503..1639)
                     /locus_tag="P5316_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRR85870.1"
                     /translation="MPKNTLLKQLARKHGTPLFIVDHDVLRRNYAEFKRRLPRVQAYY
                     AVKANPAPEIIKTLFEAGASFDVASIGEFRLVYENIRDLPPKARQDYIWDKIIYANPI
                     KDKATLEELDQYKPLVTYDNLEEVRKIRKHAPHAGLALRIRVPNTGSMVELSSKFGAA
                     PGEAADLIAAAFDAGLIVEGLCFHVGSQCTNFDNFVQALNMSAQVLEECAGRGYKLKL
                     LDIGGGFPAPYDGSVERFRDLARILNAEFRRLFPKELQILAEPGRFMVATAATLVAEV
                     IGKAERDGKRCYYLNDGIYHTFSGVLFDHCQYHIKAFKKGPTQLCAVFGPTCDALDTI
                     CQSEPLPELDLGDLVYSENIGAYSHASSTWFNGFPPAKVVHINV"
     gene            complement(1786..2943)
                     /gene="speY"
                     /locus_tag="P5316_12755"
     CDS             complement(1786..2943)
                     /gene="speY"
                     /locus_tag="P5316_12755"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006277976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HRR85871.1"
                     /translation="MARIQLPKKFTSKYPHKFAKGKQRFLCGKRILPKPITGKESLPD
                     LMDQVFLAYNAARLQEACRLFTERMLQADVTVGMSLAGALTPAGLGCSAIVPLIKAGY
                     VDWIVSTGANLYHDMHHAMNLPLFRGSPFVRDPELRKVGVVRIYDILLDYNDVLMQTD
                     HILRQILLQPEFQHEMGTAELHYLLGKYCAEFEEQTGHKDVSVLAAAYRAGVPCFCPS
                     PGDSTIGMNVAGLELRGSKLRVNPSRDVNETTAIVLDAKLKKRRTGVVLVGGGSPKNF
                     TLQTEPQIQEVLRIPEAGHDLFIQFTDARPDTGGLSGATPHEAVSWGKVDPNKLPDAI
                     VCYLDSTVALPLLTHYALARHKPRRFKRLYDRLPELRDLVSKEYWKRNKQL"
     gene            3331..3645
                     /locus_tag="P5316_12760"
     CDS             3331..3645
                     /locus_tag="P5316_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85872.1"
                     /translation="MPDKKPVDPSAHPEPSTNPLAASQLPPEQLRDALKAFKKRLKLT
                     RLDDESRLGHGAVTKGGRSGVQAIQPPLQYPKAVWDELVRQGRLKNAGHGLYTLGNER
                     SE"
     gene            complement(3715..3930)
                     /locus_tag="P5316_12765"
     CDS             complement(3715..3930)
                     /locus_tag="P5316_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRR85873.1"
                     /translation="MKPAVGTPEPGGILWQRMLGICRVITREAARVPVFDVVELAPIP
                     GFVAPDFLSAKLVYKILSYTLARSSGK"
     gene            complement(3930..4202)
                     /locus_tag="P5316_12770"
     CDS             complement(3930..4202)
                     /locus_tag="P5316_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85874.1"
                     /translation="MIRVIDLGEEGLTAEQVRDSVRSPEGAILREGGRVIARIEPADE
                     TDLDDEMWAHHPEQVARGTTARQRFERGQSIPHETLKRQLEQESEE"
     gene            complement(4186..4488)
                     /locus_tag="P5316_12775"
     CDS             complement(4186..4488)
                     /locus_tag="P5316_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRR85875.1"
                     /translation="MARFEIHYDRTAVDDLRALRRVDQVAILGAIERHLRDEPSRVSR
                     QAIKKLEPPVLAGYRLRVGDYRVFYDVYEDRQIVLVVAVRFKGRMTLDEAAHDSSD"
     gene            complement(4500..5423)
                     /gene="speB"
                     /locus_tag="P5316_12780"
     CDS             complement(4500..5423)
                     /gene="speB"
                     /locus_tag="P5316_12780"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRR85876.1"
                     /translation="MSQEGLFQTPWNFLGLPEELSSPQRARAWLLPVPYESTTSYGAG
                     TRNGPAAIIAASRQVELFDCEFGKEPAMEYGVHTMNPLALVHRSPDLMVKAIEEAVAV
                     ILSGKPRPEVLVLLGGEHTISPGVVRGIARTIGKDNLVCVQVDAHADLRDEYEGSPFS
                     HACAARRILDVCPVFQIGIRNISAGEEPFRRSCDRVHTVFAEESIAPDGAFLKDLAAF
                     VRGQQVFLTIDLDGLDPSIMPAVGTPEPGGILWQRMLEICRVITREAARVPVFDVVEL
                     APIPGFIAPDFLSAKLVYKILSYTVSSGRDQ"
     gene            6136..7317
                     /locus_tag="P5316_12785"
     CDS             6136..7317
                     /locus_tag="P5316_12785"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRR85877.1"
                     /translation="MSHTPPELATLYRDNLLDDVIPFWEQHSIDRDKGGYFTCLDRRG
                     NVFDTDKFVWLQARQVWTFSMLYRKVEQRPTWLEIARHGAEFLKRHGTDDEGNWYFSL
                     TREGKPLVQPYNWFTDCFAAMGFAQYALASGDSQAADIAVKTFHNVLRRRDNPKGKYT
                     KQVPGTRPLKSQAFPMILINVALEVEGVVPDDEIRRTVEDSIREVMTLFYDNERRITW
                     DNVAPDGSHPDCFEGRIILPGHGIETMWFMIEAVRRFGGDRAIIDKCVDAIVNLIEFG
                     WDDRYEGILYYRDIKDRPPVQLEWDQKLWWVHIETLVALAIAWSVTGRSDCRRWYERI
                     HDYTWKRFPDPQYGEWFGYLNRRGEPLLDLKGGKWKGCYHIPRGLYMCWREFEAMTTK
                     A"
     gene            7330..9120
                     /locus_tag="P5316_12790"
     CDS             7330..9120
                     /locus_tag="P5316_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85878.1"
                     /translation="MLAESAFQTLDYLVLAAYMASLVVVGFYFRKRAQKDLESYFLAD
                     RKMPGWLAGFSYAATCMNTDAITAYCGMTVISGICICWWYISRFGLALMIGAILFAVF
                     WRRLHIFTSPEFYEFRFTGAPALTMRSWVSIRSAFIAVVAWTGSGLLGIHKVLNPMLG
                     WEKWETYLLVIPVLLLYVLLSGYVGVVVTDFIQTWVMIAAAFILMGAVWGDFGGPSGL
                     YHSLVSQFGTSVVTWHPPSSHEYLGVIGILAWTVGTAVGYGGDVAPMAGAMEGQRLLS
                     CKNAREASKMYIWTEVVLFFLLAVTTMPALGAMCKWPGLHDGRINKELAYGMLLKEYL
                     PAGLLGLAVSGLAAAIMSTVSSNLNFGAQVILNDVYKRSFVRDASMSHYMNVGRIVML
                     AIMGLAVLVATAAENVIDVSVFMLGLSSAEITANWGQWWWWRFNGKARLAASFGGPVI
                     FLVNKYLVFGMLLRTRDDVGYPVVLSSIAMTFVLWVLVALLTQPDPEERLIEFYKRAR
                     PLGWWGPIAAKAGIQPRSALYIPTGLIIAAFGAIMVAAGTIAFSSAYLADWPSVAVFA
                     GICLGAGIIFKLVHKRFMRLLHADQETDEQ"
     gene            9577..10197
                     /locus_tag="P5316_12795"
     CDS             9577..10197
                     /locus_tag="P5316_12795"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85879.1"
                     /translation="MKTQNRVTLVWVAIVSLVVSQTAMAQGVGNLSSLPTQFETSIPP
                     SSGWVVVEPTGLPVPVSLDPKGPVWRKNFTGPNGGPFVYPALSPPLTVSEHLVVAGNL
                     PWTDWHEDVLDPNWTWANPVLLVNGLPAPGLTVSGVGTSSLSFFFNPVAPGSFIDISK
                     ELVYNGVPGTAFTGTLPIIQYPTPEPATLGLVALGSLLLVRRRRMT"
     gene            10508..11278
                     /locus_tag="P5316_12800"
     CDS             10508..11278
                     /locus_tag="P5316_12800"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85880.1"
                     /translation="MKTNTNRILAGAGLVLAFAVPSAWAANYTIDWLNMAPTPLGSSV
                     PNNSVFNLPGVGNVTITYSLPSSLLNTRNQNPLFQNGTVTSGPATYSWTAHELLGVTS
                     YAPNPPFVVAPWAVTYTFPGTVPAGTLYVGVAGLGRTNSFGGGMSTATVNQNGAYLGD
                     WTGGGNYGATQFIGGPGTFTMQNSITGLGGADPWWNSALGVVQILDPVSSLTVIVNQI
                     PGDGLGVNIGHAVPEPATLGLIALGSLPVALRRKRFVS"
     gene            11495..12142
                     /locus_tag="P5316_12805"
     CDS             11495..12142
                     /locus_tag="P5316_12805"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85881.1"
                     /translation="MKIDEKNVHAFSIAALIIAVGSQMSLAQGVGNLGSLPTLFLSPA
                     PITGWSVDSGNATSPWLPVQIDPNGPQWGKTFTGLNGQPIVAVPGQTFTLQELLVVAP
                     TQSWEDWHEHILTPGWEWVQPTVFLANFAPPPGLNTVVTPATPTSGGKIDFTFNPLVP
                     GTLIDIRKTLKYDDPTGAVFTGKIEIAQFPTPEPATLGLLMLGGLLALRRRRHAA"
     gene            12721..14814
                     /gene="fusA"
                     /locus_tag="P5316_12810"
     CDS             12721..14814
                     /gene="fusA"
                     /locus_tag="P5316_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRR85882.1"
                     /translation="MYRLARLRNIGISAHIDSGKTTLSERILYYCGRIHRMGDVKDGG
                     DGATMDHMELEKERGITITSAATTVQWDDKIINLIDTPGHVDFTIEVERSLRVLDGAI
                     LVLCAVGGVQSQSLTVDRQMKRYNVPRIAFINKCDRVGADPARVINEIEEKLGVEAVP
                     LQIPVGLEGNHVGVVDLITMKAVYFDGENGEKVRLDAIPGELVDAANRTRHGMLDTLS
                     LYSDEIMELMLEDKPISEELLHRVIRERTIAREITPVLLGSAFRNKGVQPLLNAVCRY
                     LPSPLDRITYAKDNENEGAEVALTADPDAAPVGMAFKIVDEPFGQLTYTRVYQGTIDK
                     GRTYLNSRTQRKQRVARILRMHADQREDIDRALAGDIVALMGVECNSGDTICDLEHNY
                     SLERIYVAEPVISLAVSVAKTADRDKLSKALGRFCREDPTFHVRTDDKTGETLISGMG
                     ELHLDVYVERLRREYKLELQVGRPQVSYREAPTQEVSFNYKHRKQTGGAGQYAHVIGK
                     LIPLPDSAERDYEFENAVSCGRIPTEFIPSCDKGFQSARGKGPLAGYEIVRVKILLED
                     GSYHTVDSSDMAFQICARDAFIEAFRRSQPALLEPIAKVEVEIPGEFQGSVVGDLSSR
                     RGIITNVEPRQNTVVVNAEVPLAEMFGYSTDVRSATQGKGTFTMEFCCYRQVPREIQE
                     EIILARKKAAAGQRS"
BASE COUNT         3166 a         4989 c         4525 g         2907 t
ORIGIN      
        1 cgcacccctc atcgcctcac cgtccccgct ccgcttccgc cccgtttctc ggaacacccc
       61 tcgtgttctg ccccgcaagc cgcgggaagt ttcgtcatgg cgcgacagaa cgatcgcccc
      121 gcagagcccg gagcagacaa gaacgacccc gaaaagacgt tcttgcagaa cgaagccaaa
      181 atcaggaagt ttggggaaaa tgggcaggag gccgacacgc ccccggcgca cggtcgatcg
      241 ctttcgacga cgcctccgtg ggctcgatga cctatgacgc ttttagacag tataaggact
      301 cataatactc tcttttgcca cctcacgaga cttcgaatca aatcaacaac ggcccatctg
      361 atgcactcgg acacccagcc ccctctacgg tcaaccgcaa gacggttcgg tgacccgccc
      421 tgcgggcgag acttctgtcc ggacgtgccg gatcacggcg gagccccggg cggcccgtct
      481 gtggttactc ccgccccgcc gtctacacgt tgatgtgcac caccttggcc ggcgggaagc
      541 cgttgaacca cgtcgaggat gcgtggctgt aggctccgat gttctcgctg tagaccaggt
      601 cgccaaggtc cagctccggc agcggctccg actggcagat ggtatccagg gcgtcgcagg
      661 tcggaccaaa aaccgcacag agctgcgtcg ggcctttctt gaaggccttg atgtgatact
      721 gacagtgatc aaagagcacg cccgaaaagg tgtgatagat cccgtcgttg agatagtagc
      781 agcgcttgcc gtctcgctcg gccttaccga ttacctcggc gaccagcgtg gcggcggtgg
      841 ccaccatgaa acgacccggc tctgcaagaa tctgcaattc cttggggaac agccgccgga
      901 actccgcgtt caggatcctg gccaggtccc ggaaccgctc tacgctgccg tcgtaaggcg
      961 cgggaaaacc gcccccgatg tccagcagct tgagtttgta gccccgcccg gcgcattcct
     1021 cgagcacctg cgccgacatg ttcagggcct gcacgaaatt gtcgaaattc gtgcactggc
     1081 tgcccacgtg gaaacatagg ccctcgacaa tcagccccgc atcgaaggcc gcggcaatca
     1141 ggtcggcggc ctcaccgggt gctgcaccga acttgctcga cagctcgacc atggaaccgg
     1201 tgttgggaac ccgaatccgc aacgccaacc cggcgtgcgg cgcatgcttg cggatcttgc
     1261 gaacctcttc caggttgtcg taggtgacca gcggcttgta ctgatccagc tcctccagcg
     1321 tcgctttgtc cttgatagga ttggcgtaga tgatcttgtc ccagatgtag tcctgcctgg
     1381 ccttgggcgg caggtcgcga atgttctcat aaaccagccg gaactcgccg atcgacgcca
     1441 cgtcaaagct ggctccggcc tcgaatagag tcttgatgat ctccggggcc gggttggcct
     1501 tgaccgcata ataagcctgg acgcgcggta gcctccgctt gaactcggca tagttgcgcc
     1561 gcagcacatc gtggtcaacg ataaacagcg gcgtgccgtg cttccttgcc agttgcttga
     1621 gtaacgtgtt cttgggcatg atccgcctcc agggtccctt gacgagccgg tggcttgagg
     1681 ccgccggtac gttggtcaaa aaccgcgggc ataaaccccg cggctcgcca caaaccgagg
     1741 ggataaaccc ctcggctcgt caagaatcgc gatgggtaac cgctcctaca actgtttgtt
     1801 ccgtttccag tactccttgg aaaccagatc ccgcagctca ggcaatcgat cgtagagccg
     1861 cttgaacctg cggggcttat ggcgagccaa ggcataatgc gtgagcaggg gcagagccac
     1921 cgtcgaatcc agatagcaga caatggcatc tggaagcttg ttgggatcca ccttgcccca
     1981 actgacagcc tcgtgcggcg tcgcgcccga caggccgccc gtatccggcc tcgcgtcagt
     2041 aaactggatg aagagatcgt gcccggcctc gggaatccgc aagacctcct ggatctgtgg
     2101 ctcggtctgc aaggtgaaat tcttgggcga tccgccgccc accagaacca cgccggttcg
     2161 ccgcttcttc agcttggcat ccagaacgat cgcggtggtc tcgttgacgt cacgcgaggg
     2221 attgacccgc agcttgctgc cccgaagctc gagcccggcc acgttcatgc cgatcgtcga
     2281 gtcgcccggc gaggggcaga aacacggcac gccggcccgg tacgccgccg cgagaacgga
     2341 tacgtcctta tggccggttt gctcctcgaa ctcggcgcaa tacttgccca gcaggtagtg
     2401 aagctcggcc gtgcccatct cgtgctggaa ctcgggctgg agcaggatct gccgtagaat
     2461 gtggtcggtc tgcatgagaa catcgttgta gtccagaagg atatcgtaga tccgcacgac
     2521 gccgaccttc cgtagttcag ggtcacgaac gaacggagac cccctgaaca gcggcaggtt
     2581 catggcatgg tgcatgtcgt ggtagaggtt tgccccggtg gaaacgatcc agtccacgta
     2641 gccggccttg atcagcggta cgatcgccga gcaacccagt ccggcagggg taagggctcc
     2701 tgccaggctc atgcccacgg tcacgtcggc ctggagcatc cgctcggtaa acagccgaca
     2761 ggcctcctgg agccgggccg cattgtaggc cagaaacacc tgatccatca ggtccggcag
     2821 cgattccttc ccggtgatcg gcttgggcag aatccgtttg ccgcacaaga aacgctgttt
     2881 gcccttcgca aacttgtgcg gatatttgct ggtgaatttc ttcggcaact gaatcctggc
     2941 cattacggtc tcctctgttc tcgctccccc accgccttgt gccgcctgta tggtgccccg
     3001 ccctccacac acgaggggct tccgacgtcc gccatgagcc tgccgtcatc gggcagcggc
     3061 gatgcccggc ctgcggcacg attccgggca tgatgccgca aaccatactg cccctctgcc
     3121 gggctttcaa gaagagcgtc tctcggcccg cctgtgatgt ggccgttttc tgattcggcc
     3181 cctcgctcgc gctcggggtt ctgaaaacgc cgcctggatt gtcacccacg cagcctcggc
     3241 acctggcaac gtggctactg gacaaccgac acctgatggg tatgcttctc tcatacgatc
     3301 aagcggttct gtcaaacgga aaaagctgcc atgccggata agaaacccgt cgatccaagc
     3361 gcccatccgg agccgtccac caatccgctg gccgcaagcc agttgccacc cgaacaactg
     3421 cgagatgccc tcaaggcatt caagaagcgg ctcaagctca cccggctcga cgatgagtcg
     3481 cggctgggcc acggggcggt aaccaagggt ggccgttcgg gcgtccaggc catccaaccg
     3541 ccgcttcaat accccaaggc tgtgtgggac gaactggtcc ggcaaggccg actgaaaaac
     3601 gcgggccatg ggctctatac acttgggaac gaacgatccg agtgagctgc cgccgcgaga
     3661 gactcattca cacatcaagg cacgccggtc atcaccactt cggttaatga gatctcattt
     3721 cccactgctc cgcgcaagcg tgtacgacag gatcttatac accagcttcg ccgacagaaa
     3781 gtccggcgcc acgaatccgg gaatgggggc gagttcgacc acatcaaaaa cgggaacgcg
     3841 agcggcttcg cgggtaatca cccggcagat accgagcatc cgctgccaga gaatcccgcc
     3901 gggctcgggg gtgccgaccg ccggcttgat tactcctcag attcttgctc cagttgccgc
     3961 ttgagggtct cgtgcggaat cgactggccc cgctcaaacc gctgccgtgc cgtggttcct
     4021 ctggcgacct gttcgggatg atgtgcccac atttcgtcgt cgagatccgt ttcgtccgca
     4081 ggctcgatcc gggcgatcac gcggcctccc tcccgaagaa tcgccccttc aggcgaccga
     4141 acggaatcac ggacctgttc agcggtcaaa ccttcttcgc caagatcaat cactcgaatc
     4201 atgtgcggcc tcgtcaagcg tcattcttcc cttgaacctg acagccacca ccagcacgat
     4261 ctgccggtct tcgtagacgt catagaacac acggtagtca ccaacacgca ggcgataccc
     4321 cgcgagcacc ggcggttcca acttcttgat cgcttgtcgg ctgacgcgac tcggctcatc
     4381 tcgcaaatgg cgctcgatgg cgccgagaat agccacttgg tccacccgcc gcagtgctcg
     4441 caggtcatcc acggccgtcc ggtcatagtg tatctcgaac cgagccattc tgccatgccc
     4501 tattgatccc gtcctgacga gaccgtgtac gagaggatct tatacaccag cttcgccgac
     4561 agaaagtccg gggcgatgaa tccgggaatg ggggcgagtt cgaccacatc aaaaacggga
     4621 acgcgagcgg cttcgcgggt aatcacccgg cagatttcga gcatccgctg ccagagaatc
     4681 ccgccgggct cgggggtgcc gaccgccggc atgatcgacg gatcgaggcc gtccaggtca
     4741 atggtcaaga aaacctgttg cccgcggaca aacgcggcca ggtctttgag gaacgctccg
     4801 tcgggggcga tcgactcctc ggcaaagacc gtgtgtaccc ggtcgcagga gcggcggaac
     4861 ggctcttcgc cggccgagat gttgcggatg ccgatctgga agaccgggca gacgtccaga
     4921 atccgccggg cggcacaggc gtgactgaac ggcgaaccct cgtactcatc tcgcagatcg
     4981 gcatgggcat ccacttgaac acagacgaga ttgtccttgc cgatcgtccg ggcaatgcct
     5041 cggaccacgc caggcgaaat tgtgtgctcg ccgccgagca ggaccagcac ctccggtcgt
     5101 ggcttgcccg aaaggatcac cgcgacagct tcctcaatcg ccttgaccat caggtcgggc
     5161 gagcggtgaa ccaaagcgag cgggttcatc gtgtgaacgc catactccat cgccggctcc
     5221 ttgccgaact cacaatcaaa aagctcgacc tgccgcgagg cggcgatgat cgcggccggg
     5281 ccgttgcgag tgcccgcacc gtaagaggtc gtggactcgt acgggacagg cagcaaccac
     5341 gctctggccc gctgcggcga cgacagctct tccggaagcc ccagaaaatt ccacggcgtt
     5401 tgaaacaagc cttcttggct catgaatcgc tcctttgcgc gtgaacgtca ctcgtccgcc
     5461 accaggccgc acgcggcacg gcccgaaaaa taacccctgc tcgcgggcga ttcaaggaat
     5521 gccgacggcc gcaggtcttc gcgggctcgt gacagaagcg gcgacactcc tggcggctga
     5581 cccggttgtt gcgaacgcaa gccgaaagca gaccgcttca gcggtctctc ccgaaaagca
     5641 aataggccgg ccgttttaag actggttcac cgtcggccaa tccttccttt tccgtgttgc
     5701 cccatcaagc ccgttttaac gggcctgtcc ttgtcaggta agctcattga aacgggctga
     5761 gaccatagaa tggaagaaga accgcaggcg accaccaacc ggctgtatca cggccggcct
     5821 atcagcccgc ctttggcaag aaaaccccct gaagggggct tccggccctc accaagtccc
     5881 ggggacgaaa caggctggaa acctgccgct cctggctgca aaacatgcca ggtccccgtc
     5941 gccgtggcga cctcgacgct gccgtactga ccggatcccg cagttttcgc gcacattctc
     6001 aagaaaggtt gacgaaagcg gtacgggcct tacgctgttg gcacgtcgtc cgcgagccaa
     6061 gcgatccgac accgaggata gtccagtcgg ccccctgccg acggcaccac aataggactt
     6121 tgcgagggca tcttgatgtc tcacaccccg ccggaacttg ccacactcta tcgcgacaac
     6181 ctgcttgatg atgtcatccc tttctgggaa caacattcca tcgaccggga caagggcggt
     6241 tactttacct gcctcgatcg ccgcggaaac gtgttcgaca ccgacaagtt cgtctggctg
     6301 caggcccggc aggtgtggac cttctcaatg ctttaccgca aagtcgaaca acggccgaca
     6361 tggctggaga tcgctcgtca cggcgctgag ttcctcaagc ggcacgggac cgacgacgag
     6421 ggtaactggt acttctccct gacccgtgag ggcaagccgc tcgtgcaacc ctacaactgg
     6481 ttcaccgact gcttcgcggc catgggcttt gcccaatacg ccctggcctc gggtgattcg
     6541 caggccgccg atatcgccgt gaagaccttt cacaacgtgc tgcggcgcag agacaatcca
     6601 aaaggcaaat acaccaagca ggtccccggc acgcgaccgc tgaaatctca ggccttcccg
     6661 atgatcctga tcaacgtggc cttggaagtc gaaggcgtcg tgcccgacga cgagattcgc
     6721 cgcacagtcg aagactccat tcgcgaggtc atgaccctct tctatgacaa cgagcggcgg
     6781 atcacctggg acaacgtcgc cccggacggc tcgcatcccg actgcttcga gggccggatc
     6841 atcctgcccg gccacggcat cgagaccatg tggttcatga tcgaagcggt tcggcgcttt
     6901 ggcggcgatc gggccatcat cgacaagtgc gtcgatgcca tcgtcaacct catcgagttc
     6961 ggctgggacg atcgatatga ggggattctc tactaccgtg acatcaagga ccggcctccc
     7021 gtccagctcg agtgggacca gaagctctgg tgggtacaca tcgagacgct tgtcgccctg
     7081 gctattgcct ggtctgtcac cgggcgcagc gattgccgac ggtggtacga gcggatccac
     7141 gactacacct ggaagcgatt ccccgatccg cagtacggcg agtggttcgg ctacctgaac
     7201 cgccgcggcg agccgctgct cgatctcaag ggcggaaagt ggaaagggtg ctaccacatc
     7261 ccgcgcgggc tctacatgtg ctggcgcgag ttcgaggcca tgacaacaaa ggcgtgagcg
     7321 acaaccggca tgcttgctga aagcgctttt cagacgctcg actacctggt gctggccgcc
     7381 tacatggcca gcctcgtcgt cgtgggtttc tatttccgaa aacgcgccca gaaggacctc
     7441 gaaagctact tcctggcgga ccgcaagatg cccggatggc tggcgggctt ctcctacgcc
     7501 gccacgtgta tgaacaccga cgccatcacc gcctattgcg gcatgaccgt gatctcgggc
     7561 atctgcatct gctggtggta catcagcagg ttcgggctgg ctctgatgat cggggccatt
     7621 ctcttcgcgg tgttctggcg ccgacttcat atcttcacct cccctgagtt ctacgagttc
     7681 cgatttaccg gcgcaccggc ccttaccatg cggtcgtggg tttccatccg cagcgcattc
     7741 attgccgtgg tcgcatggac cggttccggc ctgctgggaa ttcacaaggt gctcaacccg
     7801 atgctgggct gggagaagtg ggaaacctac ctcctggtca tccccgtgct gctgctctac
     7861 gtgctgttgt ccggctacgt gggtgtggtc gtgaccgact ttatccagac ctgggtgatg
     7921 atcgccgccg cgttcatcct catgggcgcg gtctggggcg atttcggcgg accgtcgggc
     7981 ctctatcact cgctggtgag ccagttcggc acatcagtgg tgacctggca ccccccttcc
     8041 agccacgagt acctcggcgt catcggcatc ctcgcctgga cggtgggcac ggccgtgggc
     8101 tacggcggtg acgtcgcccc gatggccggg gccatggaag gacagcgcct tctgtcatgc
     8161 aaaaacgccc gcgaagcgtc caaaatgtac atctggaccg aggtggtgct gttcttcctc
     8221 ctggcggtga ccacgatgcc cgcgcttgga gcaatgtgca agtggccagg cctccatgac
     8281 ggccggatca acaaagaact cgcatacggc atgctcctca aggagtatct cccggccggg
     8341 ctcctgggtc tggcggtcag cggcctggcg gcggccatta tgtcgaccgt cagctccaac
     8401 ctgaacttcg gcgcacaggt tattctcaac gacgtgtaca agagatcatt cgttcgtgac
     8461 gcctccatga gccactatat gaacgttggt cgcatcgtca tgctcgcgat catgggcctc
     8521 gccgttcttg tggccaccgc agccgagaac gtcatcgacg tttccgtgtt catgctcggc
     8581 ttgtcctcgg cggagatcac agccaactgg gggcagtggt ggtggtggcg attcaacggc
     8641 aaggcaaggc tcgcggcctc gttcggcggc cccgtaatct tcctggtcaa caagtatctg
     8701 gttttcggaa tgctgttgag aaccagggac gacgtgggtt atccggtcgt cctgtcctca
     8761 atcgccatga ccttcgtctt gtgggtcctg gtcgccctgc tgacccaacc ggacccggaa
     8821 gaaaggctga tcgagttcta caagagagca cggccgttgg gctggtgggg tccgatcgcg
     8881 gccaaggccg gcattcagcc gcggtcggcc ttgtacatcc ctaccggcct gatcatcgcg
     8941 gccttcgggg cgatcatggt tgcggccgga acgatcgcgt tcagctccgc ctacctggct
     9001 gactggccga gcgtcgctgt gttcgcagga atatgccttg gcgctggcat catcttcaaa
     9061 ctggtccaca aacgattcat gcggctgttg cacgccgatc aggagacaga cgagcaatag
     9121 ccccgccgga gagttccgct ctcccacgga cccggcagcg aaagaaaaag accccggctg
     9181 tttcatgtct gcactgccgg cggacttcgc agaagcgtga accaacgaaa ccgccacccg
     9241 cgataatccg cctgaacatc aagacgacgc gatgatccat tcacttgatg cccaaggaca
     9301 agcaggcgac ggcagagtct ctatcgctca cttccgacca tgcgcgagcg catgtgcgat
     9361 acccatagcc cgaaaccacc cgatcaattg tgaaagagtt cacaaagaac accagaaccg
     9421 gcctgcggca cgacacaaac gctgttcatg agcgtaaaca tgccattccg ctggtcaacc
     9481 catcagaccc tacctagctt tactggtaga ccggtgagag gcaaacaatc gtttctccac
     9541 cgaagatgaa gaaaaagcag attaaggagc acagcaatga aaacgcagaa cagggtcaca
     9601 ttggtttggg tcgccatcgt cagcctggtc gtctcccaaa cggccatggc acagggcgtg
     9661 ggcaacctga gttcattgcc cactcagttc gagacctcga tcccgccctc ttccggctgg
     9721 gtcgtggtgg agccgaccgg gctgcccgtt cccgtttccc tcgaccctaa gggacccgtg
     9781 tggcgaaaga atttcaccgg ccccaacggc ggtccgttcg tatatcccgc actcagcccc
     9841 ccgctgaccg tatccgagca tctcgtggtg gcaggcaatc ttccgtggac cgattggcac
     9901 gaggacgtgc tggatcccaa ttggacctgg gctaatcccg tccttctggt gaacggtctc
     9961 ccggcgccgg gactcactgt ctccggggtt ggtacaagca gcctctcctt cttcttcaac
    10021 ccggtcgcgc cgggttcatt catcgacatc agcaaggagc ttgtctacaa cggagtgccc
    10081 ggaacagcct tcaccggcac gctgccgatc attcaatacc ccacgccgga gcctgccaca
    10141 ctgggactgg tcgccttggg cagccttctt cttgtgcgtc gccgccgaat gacctagcca
    10201 ccttttcggc agctcaattg cacgccgtcg ggccgggccg ctctcagaac catcggggcg
    10261 gacatctatg cggcttgcgc gcatgtttct ggctcgcagt gcgtttggct cacggcccac
    10321 aaaaaggcgg tccgccttag gggcgtcggt agacaagcac acggtcaacg ggagccactg
    10381 aattcgtgaa agccgccaag tgaataggac attcagacca accgtagtaa tcgaggagtg
    10441 cccctggggt gtacccgggg caggagattc aaaggatcca agccataatg aacggagaaa
    10501 cacaaacatg aaaacgaaca cgaaccggat tctcgccggt gccggcctgg ttctggcctt
    10561 cgccgtcccc agcgcttggg ctgccaatta tacaattgac tggctcaaca tggcgccgac
    10621 gccgctcggc tcgagcgtgc cgaacaactc cgtgttcaac ttgccgggcg tcggaaacgt
    10681 cacgatcacg tattcgcttc cttcctcgct cttgaatact cgcaatcaga accccctgtt
    10741 tcagaacgga accgtgacca gcggccctgc tacctactca tggaccgccc acgagctgct
    10801 cggcgtaacc agctacgcgc ccaatcctcc gtttgtggtc gccccatggg cggtcaccta
    10861 cacgttccca ggtacggtcc cggccggcac cctctatgtc ggcgtcgcag gacttggtcg
    10921 gaccaacagt ttcggcggcg ggatgtccac ggcaaccgtc aatcagaatg gcgcgtatct
    10981 gggtgattgg acgggcggcg ggaactacgg cgccacgcaa ttcatcggcg gccccggtac
    11041 gttcacgatg caaaactcga tcacaggcct gggcggcgcc gatccgtggt ggaactcggc
    11101 gctcggcgtc gtgcaaatcc tcgacccagt cagctcgctg actgtgatcg tcaaccagat
    11161 ccctggtgac ggtctgggcg tgaacatcgg acacgccgtc ccggagcctg cgacactggg
    11221 cctaattgcg ctgggcagcc tgcctgttgc gcttcggcgc aaacgatttg tgtcttgaca
    11281 ccccccgcca tcgcggtcgg aggcgctatg gcgacagact gaggttggaa caaaaagaca
    11341 gcgagcaccc ctacccaccc gaaccgtcgg gcctcacgat cctgtgcctc ggatgggcgg
    11401 gcgggtgctc acaaaagctc cgctatcacg tgatggcgag ccgaaaagat ccttgccaga
    11461 gcagggcaca aagccctctt gaggagaaga taacatgaag atcgacgaga agaacgtgca
    11521 cgcattttcg attgccgccc tgatcatcgc tgttggttcg cagatgtccc tcgcgcaggg
    11581 cgtgggcaat ctcggatcgt taccgacttt gtttctctca cccgcgccga tcaccgggtg
    11641 gagcgtcgat agcggcaatg ccacaagccc ctggttgccc gtccagatcg atccgaacgg
    11701 cccgcaatgg ggcaagacgt tcaccggcct caacggtcag cctatagttg cggtgcccgg
    11761 ccagacgttc accctgcagg aactgctcgt cgtcgccccg acgcagagct gggaggactg
    11821 gcacgagcac atcctgacac ccggctggga atgggtacag ccgaccgtct ttctcgcaaa
    11881 cttcgctccg ccgccgggcc tcaacacagt tgtgaccccg gcgacgccga ccagcggcgg
    11941 gaagatcgac ttcaccttca acccgctcgt tcccggtacg ctcatcgaca ttcgcaagac
    12001 gctgaagtac gacgacccga cgggcgcggt cttcaccggg aaaattgaaa tcgcccagtt
    12061 tccaacgcca gagcccgcta cgttagggct gttgatgctg ggcggtctgc tcgctctgcg
    12121 ccggcgcagg catgctgctt gatcgctgca aaggatcaac tccgcggttc ctgaatcaag
    12181 aaggtcggcg gtcaagaccg gaaatgacgc cgccttcttg gttctaaaca gccgtcataa
    12241 taaccgttcc agcccgccag agacgtccga gaaaacctcc tgcgcggcat cggagcccgg
    12301 ccggcccgca tccccatggt tccacaaccc catcgccaaa ggcaaagcga gcccttgaat
    12361 tctgccaacg gccaggatag actcttggat cgtagccaac ctgcttggcc cggcagccgg
    12421 tcgctccggc ccggaacggc agcaagaggt gatgccatga tcgagtacac cgtgagggtg
    12481 attctcgaac tcaggcccgc ctgatcgacc gcccatgacc gcggccgact caggccgccg
    12541 gtcgggattc ccccttttga cagaaaacaa acggggccct gaataagggt caatgatagc
    12601 gtggtgaaat gcccgtccga caaaaggctt gtcaccagac cagggtggat ggccaccttc
    12661 gcgatggtgg agacggcaac acagagagtt cagtagcgac gcaccggaaa gcaacacaag
    12721 atgtatcgat tggcacgact aaggaatatc ggaatctccg ctcacatcga ctcgggtaag
    12781 acaaccctga gcgagcgcat cctctattac tgcggacgca tccatcggat gggcgacgtc
    12841 aaggacggcg gcgatggggc taccatggac cacatggagc tggaaaagga acgcggcatc
    12901 accatcacct cggcggccac aaccgtccag tgggacgata agatcatcaa cctgatcgac
    12961 accccagggc acgtcgactt caccatcgag gtcgagcggt cgctccgcgt gctggatggc
    13021 gccattttgg tgctgtgtgc cgtgggcggc gtccagtcgc agtcactgac ggtcgaccgc
    13081 cagatgaaac gctacaacgt tccgcgtatc gccttcatca acaagtgcga tcgcgtcggg
    13141 gcagaccccg cccgggtaat caatgagatc gaagagaagc tcggggtgga agcggtgccc
    13201 ctgcagatcc ccgtcggcct ggaaggcaac cacgtgggcg tcgttgatct gattaccatg
    13261 aaggccgtct actttgacgg ggagaatggt gaaaaggtcc gtctcgacgc gattcccggt
    13321 gaactggtcg acgccgcgaa tcggacccgt cacggcatgc tcgataccct ctccctctac
    13381 agcgacgaga tcatggaact tatgctcgaa gacaagccga tcagcgagga actcctccac
    13441 cgagtcatcc gcgagcgaac catcgccagg gaaatcacgc cggtcctgct cggctcggcg
    13501 ttcagaaaca aaggcgtgca acctctgctc aacgccgttt gccgctatct gcccagtccg
    13561 ctggaccgga tcacctatgc caaggacaac gagaacgaag gggcggaggt ggcgctcacg
    13621 gccgatcccg acgccgctcc cgtcggcatg gcattcaaga tcgtcgacga gcctttcggc
    13681 cagctcacct atactcgcgt ctaccagggc actatcgaca agggacggac gtacctcaac
    13741 agccgcacgc agcgcaagca acgcgtcgcc cggatcctac ggatgcacgc cgaccaacgc
    13801 gaggatatcg accgggccct tgccggcgac atcgtggctc tgatgggcgt tgagtgcaat
    13861 tcaggcgata ccatctgcga ccttgaacac aactactcgc tcgaacgcat ctacgtggcc
    13921 gagcccgtca tatcgctggc cgtcagcgtc gcaaaaaccg ccgaccgcga caagctgagc
    13981 aaggccctgg gccgattctg ccgcgaggac ccgacgttcc acgtgcgcac cgacgacaag
    14041 accggcgaaa cgctcatctc gggcatgggc gagctgcacc tggacgtcta cgtcgaacgc
    14101 ctgcgccgcg agtacaagct tgagcttcaa gtcggacggc ctcaggtcag ctaccgcgag
    14161 gcgcccacac aggaagtctc gttcaactac aagcaccgca aacagaccgg cggcgcaggc
    14221 cagtacgccc acgttatcgg caaattgatc cccttgcccg acagcgccga aagggactac
    14281 gagttcgaga acgcagtcag ttgcggacgc attcccaccg agttcattcc ctcctgtgac
    14341 aagggttttc agagtgcccg cggcaaggga ccgctggccg gctacgaaat cgtgcgggtg
    14401 aagatcctgc tcgaagacgg cagctatcac accgtggact cgtccgacat ggcctttcag
    14461 atctgtgccc gcgacgcctt cattgaggcc tttcggcgat cgcaacccgc tttgctggaa
    14521 ccgatcgcca aagtggaagt cgaaatccct ggcgaattcc agggctccgt agtcggcgat
    14581 ctgtcgagcc gcagaggtat catcaccaac gtcgagccgc ggcaaaacac ggtagtcgtc
    14641 aacgccgagg tcccgctggc cgagatgttc ggttattcga ccgacgtccg ctcggccacg
    14701 cagggcaagg ggacgttcac gatggagttc tgctgttacc gacaggtacc gcgagaaatc
    14761 caggaggaga tcatcctggc ccgaaagaaa gccgccgccg ggcaacggtc atgaacggat
    14821 ggcctcccgc ttcccccctc accaaggggg gattgagcgg gggcgagaag ggagcgatct
    14881 gcgcgccgct gggccgagta ggcatgcttt gcacgccgaa gtccgtaggg cgagctctgc
    14941 acgccaaaag cgtcgggtaa agcccgacct gccgaactgg acgaagtgat cagttatcag
    15001 tgagaggaca cgaccgatcg cgaacactga gaactgacga ctgagaactg ataactcgaa
    15061 agaacgattg cttccgtgtt accagcgtga agcatcaccg acgcccgcgg ccaacccgcg
    15121 ggcgtctctt tgcgcgacac gacgttcaac aggtggcgga tgcaggtcgc cctgctgctc
    15181 gccatcctga ttcgatggcc ccacatccca tctgggacct ccgggggcga ggattcctat
    15241 catcatccta ccggacgtgg gtcgaaacgg tgggtcggca ggtcgaaacg cggacgtggc
    15301 taatctgtga gaagcggtca gcggtcggca ttcagcgatc ggctttcagc gaccggagaa
    15361 acagtgtttc accgcagcgg acgcagagga atgcggagac ctatctcagt tgtctccgtt
    15421 tcctcatgtt caaatgcctt cctcctgttc ttcatctgcg tcaatcccat gatctgccgg
    15481 ccgggactcg ctttctctct cggcgttttc gattgaagca ggtagggcag gttcgctcgg
    15541 cccggaatcc tttccgggcc gaatcctctg cggaatcctt tccgcat
//
LOCUS       DAOTPW010000132        15284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3839_length_15284_cov_5.941231, whole genome shotgun sequence.
ACCESSION   DAOTPW010000132 DAOTPW010000000
VERSION     DAOTPW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3839_length_15284_cov_5.941231"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1263
                     /locus_tag="P5316_12815"
     CDS             181..1263
                     /locus_tag="P5316_12815"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRR85883.1"
                     /translation="MARYVIARRLSVGLMSATLALTAGCGGWPLGYIPSVVIGELAFL
                     GRRVPVEWALNDPTLSEEQKDKLAFLLRARDYCRDVVGLEVGNNFQTFVNLHGQALAW
                     NLTASRKDAITPYYWYVPFAGPLPYLGFFNLDKAFSEKDRLVAAGYDTFMYEVDAFAI
                     PFLPDPMTSRLLERSYGTLADTVAHELTHNTVMSMKDITFSESLAVFVGRTVAVEFLA
                     VEFGQDSPLIRETLEAYEDDDVFRLFLRDLTEELNDLYGSDLSSEQKIAARVGVFEAW
                     RRRFAEEALPLMHFPEDYHAYTENLLNNAFLVVDRRYYSNQRLFEQAFELVGRDWSRA
                     LDLFSAAANSDNPMQYLEDVVNGDNP"
     gene            1518..2366
                     /locus_tag="P5316_12820"
     CDS             1518..2366
                     /locus_tag="P5316_12820"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRR85884.1"
                     /translation="MPKERLQKVLAAAGLASRRECEQIILDGRVSVNARKVHSLPVLV
                     DPDVDKIAVDGRPLRAQRKVYFLLHKPKGVHCTNYDPDGRPRAVDLLGGVRERVFPVG
                     RLDADTTGLLLMTNDGELAQRLTHPRHGIPKTYRAHVNGLITREQLDELRKGVWLAEG
                     KTRVSEATVIHAARDQSVVEITLREGRNREVRRVLAKIGHAVRKLIRIRIGPLSLRGL
                     GPGEFRPLTSSEVNLLKNYKPHPERRAKTTPADTGNHQMTPRKHHGRTGHRGLQRKIP
                     RRRSRD"
     gene            2436..3701
                     /locus_tag="P5316_12825"
     CDS             2436..3701
                     /locus_tag="P5316_12825"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR85885.1"
                     /translation="MNEQISRRKLLKHGAAAIASAALVSGCMQPVGVTLTNNHPADAP
                     SDPDSPDKVVLGLFPRDGQSSPQMAVRNACRQLDWSWLRRGDSVFVKLSSNSAHPHPA
                     ATSPNAVRAIVTELLDRGAGRVLVGDQGGVEYVRLVDGDKRHGSTETLMRKNGLHEAI
                     VDSGARPCFFDDYGYETGYFQASLPFDNPHWREPPYVARIIREVDHIIYLPRLASHVL
                     AGYTHGHKIAVGWLRDDSRFQMHFEAGSFHEKYVEVNYIPDIRQRLRLVITLAEQVLL
                     NVGPDVGTLATPDSWIVIASSHLANHDAVSVAVLAYVDGKTANGVHFIPPAYGTLSNT
                     ANWTFLSQIVPAQTGIPWGKPRMLTYQKLRTHRYQTGIASDLALTRAYQILGGVPRTI
                     AVHTTGRAPDAEFRSFLRAYLSGVAGRSG"
     gene            complement(4263..7262)
                     /locus_tag="P5316_12830"
     CDS             complement(4263..7262)
                     /locus_tag="P5316_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRR85886.1"
                     /translation="MICRNLLAAVTLIAILGLPPAFAGLSEYQDAVHAEPSLISYFTF
                     DADSGTAAADTCTTALPNPTGALQGTATFTPTGEGFGGAGKALSLTGAGWVNFGAVAE
                     FAFGGQSAELATPGTVEMWVRPSWDPLTPPSYNPALASVREVTPGNDPNPYTRWSMHL
                     EKTRLGWGVFGGIMGYHPEYWNFRPDQWYHVAFVFTPNYQNNTNYPTTRVFVNGILLG
                     DFWGYLGAKTDAPFQIGAADPNGGSAFIGLIDEVAVYSTWLSDSAIAQHYLAAVGVAP
                     PTTPLPSRAAYGAQVMADAPIGYYSFEGDTISVTDNSGNGRHGTLVEPAKLRWTSGFG
                     GGQALAFDNSGSSGGMVSMGAVEDFTFADGTGTVEAWVAPAPGIKYRSDNFSWPFGNY
                     RPGIASCADDYWHPYYALEMRTSDATIGVENVPEWYVNGPIPGGLPEDEHWFHVVAVF
                     NAGKLDYYVNGLQVINQADFQLLPPDGFLGNFQIGAIDPQGDWGFPGKIDEVAVYDAA
                     LSPERIAAHYSSVAMTFDIHEIEFLDPTNLTPVDVTLSAPSGQSSSGLELTFSYNPAI
                     ITVTVPALSLTLTPGVAYPIPDGTTSAVLRVSPVSGGYGTTMLTATPNAASGWLAGDS
                     IQIQSSLVIVGGMYVNDHFDDNAVGTNVNGVGGGWYVPYQAKGAPSESDSSLKILNNS
                     GWYAWTGIASKSAGEFPFMNEDGIRIEWVINYVQLTAAGGASGAPNSDGAECFHELGV
                     ISANAENNQWNELYRNTKGGLYVNLFYSGYVDSPTENITVKGSIRVVNSNHPEARSDE
                     GANGLETPVVFELTNVHSITPAKPLVVTIELDQDGWKVGFPQNAGAVYSVWPTGTSRP
                     GLTIDDVSGKVLGGWDANTLGAGLAGAAITNEFDNGAFLFAAFHNIVDGNGQGWIDSV
                     KACVGCPLATDCPNPFADADGDGDVDQADFAQLQACFTGTGGGIPRGCGCLDHDGSGS
                     IDLPDLDAFEICASGAGVPAAKACDDE"
     gene            complement(7562..8731)
                     /locus_tag="P5316_12835"
     CDS             complement(7562..8731)
                     /locus_tag="P5316_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HRR85887.1"
                     /translation="MTRARRVALLIGQDIGFCRRVLQGVHDYAITRGWVFHDARADIR
                     ALRPMRMWKPDGVICSVFDEDVARRLACCHVPVVNTSSMITTWKGPLVDVDHLEVGRL
                     AAEHLLDRGFRNFGFLGSADGGCSMGRAAGFSKRLAQAGFTPFVCHAEYRPVPVLDAS
                     WQGLSRSVQSWLLRLPKPVGVLASHDKPGRDLVDTCHQLGLRVPDEVAVVGVDDDEFE
                     CRLCHPPLSSVHNPGVQIGYEAARLLDRLLSGESPRQLRLSIPPTHVVARQSTETTAV
                     EDPDVAAALIWIRDHLSEDISVDGVVEAIGTCRRTLERRFRAAVGDSILEKIRRMRIE
                     KAKHLLAETDLKLSVIAQQCGIGSAARLTVLFKQVTGYQPSLFRRTAANRSNHRE"
     gene            9355..10191
                     /locus_tag="P5316_12840"
     CDS             9355..10191
                     /locus_tag="P5316_12840"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HRR85888.1"
                     /translation="MPCPRTMSVDSLVVRRGARPARRGGNPVRQPGFTLIEVLVVVSI
                     IALLIAILLPSLSKAREQSRRVVCQNNLRQLQHAIVFYLSDHKGIFPPHRTKVQAGTK
                     GRDDLGEWAWFQQLERYTKSPEIPHCPTLANHTQEDSGIVWSWAYNRLDIGYGYNAWF
                     LGLWNHATNGGYEEYAGLRSHPWFSESRVKSPSLNILLADANPKNDRLFGGQLWWPFV
                     DGDTGGTGEGVNVRRHQKGGNIVFNDGHTEYRRAGTINPPAGSPNKFLQYWDPLLRRA
                     KQ"
     gene            10440..11273
                     /locus_tag="P5316_12845"
     CDS             10440..11273
                     /locus_tag="P5316_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85889.1"
                     /translation="MKNSIWFVSVLAVLVAAGAANAVPNVPPGVTGLWRFEARDPVTQ
                     RATMKATIGVDMTSSHPDNSAWMVGPWTVIEPGLSDNGIAQERSWDYLTVNPSFTPNG
                     GGSYVNEYTVAIDYVQTSGLTGWNSLFQTSWGGNDSDGDLFTDGAGHIGIGAAGYSTL
                     TYDASKWHRIVWSIDNGSFFRVYVDGVLFLDGTPQPIDGRFSLYPDRFHLFADDSWED
                     GWGLVGTVAVWNRALTSAEVAGMGGWIDGADMPTPLEIPEPATLGLLLGGLPLLRRRR
                     A"
     gene            11433..13790
                     /locus_tag="P5316_12850"
     CDS             11433..13790
                     /locus_tag="P5316_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR85890.1"
                     /translation="MQRIGRLTISIWTACLCLIWSAGPVAARTLGQWDFDGGDLSATS
                     GVAMEYYGGTTASKTQFGTTVSFGIPNIAGSVASVMKFPACLPSEGYVMHTNAPGNGG
                     GIYINQYSLVLDLLYPAASTNAWRAILQTSRTNGNDADFFVGNGTTTPNANGIGVAGQ
                     YHGAIQPNTWYRVAVTVDLTTLTMAKYINGSLVGVQSLADSLDGRWSLYSTNTAPDWL
                     LLFADNDNDTKTGYVNSIQFRDYVMSAPEIAELGGPSANGIPLPVAPTNLQLVSPNGG
                     EYWPAGSTQVVSWTVTDPSGVVNVELYDGASLRAPLGQAPMVDGRFDWSISPYLGDSA
                     DYRVKLSAAAFPAVSDASDAPFEVYGSAPEPTVITKLPMLQDARPDAMNLIWETSSFG
                     NPNRVDFGLNDVSENTIANVSTQQLDATHFVHAATIQPLQIETVYKYRVRSGTATSPT
                     FTLRTAPRPGTPIRIVWFADEQDYTIFRQQVPHMAARKPDLVLAAGDLMNDGASIAQW
                     QDYWFGPLQLGNLAQTTPVLFCRGNHDGEGALAYAYSALPGNEAWFAFTYGSSRFIFL
                     DTNIETAEQTAWLENELASTEAQRASFRVVSFHKPPYTDLWDGPGYNGEAFVRENWVP
                     LFEQYKVDVVVSGHTHAYLRGMNDNVMYVIVGGAGNVVDTYTGYVWGFFTVKQSLHHY
                     GVMEVDGYALTWNAYDVNDVLFDSYGLASQTPFCPADFDGDTDVDEDDFVLFRPCLSG
                     PHAPPAADCSQEDIDADGDVDQTDYGLFQRCYSGTNEVAAANCAQ"
     gene            complement(13806..>15284)
                     /locus_tag="P5316_12855"
     CDS             complement(13806..>15284)
                     /locus_tag="P5316_12855"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRR85891.1"
                     /translation="VIALLTNYFKYINRTPPDWPDTEWRGVRAMENVVSAFWLYNRTG
                     DAEILKAADSIHASCFDWTPFFLDFPYTTEALKQGLKYTHFTHDVNNAMAVKNPALWY
                     LRSGDERDRQATFRGIASLDEHHGQVAGGFGGDEHLSGKRPTQGTELCGVVEYMFSME
                     KLVEVLGDVAFADRLERLAYNANPGTCMPDYWAHQFDQQANQVLCTIAKRQWSHGNDD
                     TSNIYGLEPNYGCCTANMHQGWPKFVSHLWMATHDQGLAAVAYGPCKVTAKVAEGEKV
                     SISEETAYPFDGVVRLVFESSRPTRFPLHLRIPVWATGARVACGGTETPVEAGRFAVV
                     DRTWDPGEAVTISLPMKLRTETRYNNSVAILRGPLCFSLKIGERYERLRSHHDKFPVN
                     DWAVYPTTPWNYGLLLDRNSPEQSVSVVTRTVGKIPFAQGVAPVTLKVRGRAIPGWDL
                     EHNSAGDPPVSPVFSNEPIVDLELIPYGCTRLRITEFPTIRE"
BASE COUNT         2985 a         4761 c         4627 g         2911 t
ORIGIN      
        1 taggaagata cggaaaagcc gccgaagtcc tgggcgggct catgttgatc ctgatcggag
       61 tcaggatttt cttccagcat tcggtgcggt tgtgacggtg gagccggctg ccggcgatct
      121 ccgccacgtg gtgacccgct ataatcagcg cgtcccgctg ccactgaggg agttgcctga
      181 atggcccggt atgtgattgc ccgccgactg tcggtcggct tgatgagtgc aacccttgca
      241 ttgaccgccg gttgtggagg atggcccctc ggctacatcc cctcggtagt cattggtgag
      301 ttggctttcc tcggtcggcg ggttcctgtc gagtgggccc tgaacgatcc gacgctctca
      361 gaggaacaga aagacaagct cgccttcctc ctgcgagcaa gggactactg ccgcgacgtg
      421 gtcggactcg aggtgggcaa caacttccag accttcgtca acctccatgg ccaagcgctt
      481 gcctggaacc tgaccgcttc gagaaaagac gcgattacac cctactactg gtacgtcccc
      541 ttcgccggcc ctcttcccta cctcggcttc tttaatctgg acaaagcatt ttctgaaaaa
      601 gaccggctgg tcgccgccgg atatgacacc ttcatgtatg aagtggacgc ctttgcgatc
      661 ccttttctgc ccgatccgat gacctctcgg ctgctcgaac gaagctacgg caccttggcg
      721 gacaccgtag cccacgaatt gacgcataac acggtcatga gtatgaagga catcaccttc
      781 agcgaatcat tggccgtttt tgtcggtcgg accgtggccg tcgagttcct ggcggttgag
      841 tttggacagg attcgcctct gatcagggaa accctcgagg cgtacgagga cgacgacgtc
      901 ttcaggttgt ttctgcgcga cctgaccgag gagctgaacg acctttacgg tagcgaccta
      961 tcgtctgagc agaaaatcgc ggcgcgagtg ggcgttttcg aagcctggcg ccgccgcttc
     1021 gcggaagaag cccttccgct gatgcatttc cccgaagact accacgccta caccgaaaac
     1081 ctgttgaaca acgctttcct ggtcgtcgac cggcgatact acagcaacca gcggctcttc
     1141 gaacaggcgt ttgagctggt tggccgcgat tggtcacggg cccttgacct cttctccgcc
     1201 gccgccaaca gcgacaaccc gatgcaatac ctcgaagacg tcgtgaacgg cgataaccca
     1261 tgagttcctt gcccgctctg ccgcgcatgc cctttgccgc ttcgcaacct caggcacggg
     1321 cagaaacaga cggcagctcg actgttgccc tggccggcgg tgggacacta cgatattgtt
     1381 gaaaacggat gtcatcgatc gcgcgggcaa gatgcgcgaa ggtacatgcg cgaccgtcag
     1441 cgaatgcgac gtcggccaat cccgatgggc tgacaatccg ctcgccgcga agaaagcgga
     1501 ccgcgaggga aggatacatg ccgaaggaaa gactccagaa agtgctcgcg gcggcgggcc
     1561 tggcctcgcg tagagaatgt gaacagatca tcctcgacgg ccgggtgtcg gttaacgctc
     1621 gcaaggttca ttcgttacct gtgctcgtcg atcccgacgt cgacaagatc gccgttgacg
     1681 gtcggccgct gcgggcccag cgcaaggtct atttcctcct gcacaaaccc aaaggtgtgc
     1741 attgcacgaa ctacgaccct gacggacggc cccgggccgt cgatcttctc ggcggcgtcc
     1801 gcgaacgcgt ttttccggtc ggaaggctgg atgccgacac caccggcctg ttattgatga
     1861 ccaacgacgg cgaactggcc cagcgattga cccaccctcg tcatggcata cccaagacct
     1921 atcgtgccca tgtcaacggg ctgatcaccc gggaacagct cgatgagctg cgaaagggtg
     1981 tctggctggc ggaaggcaag acccgcgtct cggaggcaac cgtcatccat gccgcccgag
     2041 accagagcgt cgtcgaaatc acgctgcgtg agggccgaaa ccgcgaggtc cgccgcgttc
     2101 tggccaagat cggccacgcg gtccgcaaac tcatccgcat ccggatcggg ccgctgtcgc
     2161 ttcgcgggct ggggcccgga gaattccggc ctctgacttc ttcggaagtc aatcttctga
     2221 agaactacaa gccccatccg gaacgtcggg cgaaaacaac gccggcggat accggcaacc
     2281 atcaaatgac gccgcgcaag caccacggcc ggacgggtca ccgagggctg cagcggaaga
     2341 tacctcggcg acgatcgaga gactgatgtc ttttcatgca ggcacgagga ctgagggaat
     2401 ctgtcaacca cgaggagatc cgaggaattc aaaacgtgaa cgagcagatc agcaggcgca
     2461 agctattgaa acacggcgcg gccgcaatcg catcagcggc gcttgtctca ggttgcatgc
     2521 agccggtggg cgtaaccctg accaacaatc atccggcaga cgcaccaagt gatcctgatt
     2581 cgcccgacaa ggtggttctc ggtctgtttc cgcgagacgg ccagtcgagc ccgcaaatgg
     2641 cggtccgcaa cgcctgccgc caattggact ggtcatggct gaggcgcggc gattccgtct
     2701 tcgtcaagct gtcatcgaac tcggcgcatc cgcacccggc cgccacctcg ccgaacgccg
     2761 tgcgcgcgat cgtgaccgaa ctgctggacc gcggggccgg acgcgtgctc gtcggcgacc
     2821 agggcggggt cgaatacgtt cggctggtcg atggagacaa gcggcacggc tccaccgaga
     2881 ccctgatgcg aaagaacggc ctccatgaag ccatcgtcga ttcgggtgcc aggccgtgct
     2941 tcttcgatga ctacggctac gaaacgggat acttccaggc ctcgctgccg ttcgacaacc
     3001 cacactggcg ggagccacca tacgtggcca ggatcatccg cgaggtcgac cacatcatct
     3061 acctgccgcg acttgcgtcg cacgtcctgg ccggctacac gcacggtcac aaaatagcgg
     3121 tgggctggct tcgcgatgac agccggttcc agatgcactt cgaggccggc agctttcacg
     3181 agaagtacgt cgaggtcaac tacatcccgg atatccgcca acgcctgcga ttggtcatca
     3241 ccctggccga gcaggtcctt ctcaacgtcg gtcccgatgt cggcacgctc gccacacccg
     3301 attcctggat cgtcatcgcc tcgtcgcact tggccaacca cgacgccgtg tcggtcgcgg
     3361 tattggccta tgtcgacggc aagacggcga acggagtaca tttcattcct ccggcctacg
     3421 ggacgttgtc gaacaccgcc aactggacgt tcctctcgca gatcgtgccc gcccaaacag
     3481 gcatcccctg gggcaagccg cggatgctga cctaccagaa gctccgaact caccgctacc
     3541 agaccggcat tgcctccgat ctggccctga caagggcata tcaaatcctc ggcggcgtac
     3601 cgagaaccat cgcggtccac accactggtc gggccccgga tgcagagttc cgatcattcc
     3661 tgagggcgta tttgtctgga gtcgccggcc gttccggatg agcgttggga aacggcggcc
     3721 ggggcctttt gtacgatgat ctgcagagtc cccgtggaaa cggatctgcg cgcccgggtc
     3781 ggcggtgctc agctccccga ccgaccgccc gcgcctcaca tacaaccact ctctcgaacg
     3841 gaggcggaaa cgtccgcatc aaaaccttcc gtcgcaactg gcgtacattt tgcgtgcgtg
     3901 ttttggttat gggcaaaggc cgcttgggaa ccttgcagcc cgatgtctgt gcggcttgcg
     3961 cgcatgtttc tggatcgcag agcgcttggg ttgcggcccg caaaaaggcg gtccgcctca
     4021 ggtggcggga atctcgtcgc ggcaaccggc gtccacggtc agccggacga ccgggttccc
     4081 tttctgggtg atcggaaagg ctgcggaaca gttggatgtc gccttcgcca ccgaccaagg
     4141 cggcctttgg ccgaaaccgg agcattcacc cagaacgtac acctgtcacg acgacggaaa
     4201 ccgaagaggt taagtcgcct tggccgggcc gcctgcaaat caggccgctg caccgggcgc
     4261 cgctactcat cgtcgcaggc cttggctgcc ggaacacccg cgccgctcgc gcagatctcg
     4321 aacgcgtcga ggtcgggcaa gtcgatgctg ccgctcccgt cgtggtcgag gcatccgcag
     4381 ccacgaggaa tgccgcctcc tgttcccgtg aaacaggcct ggagttgggc aaagtccgcc
     4441 tgatccacgt cgccgtcccc gtcagcatcg gcaaatgggt tggggcagtc cgtcgccagc
     4501 gggcagccga cgcaggcttt gacgctatcg atccagccct gcccgttgcc gtcgacgatg
     4561 ttgtggaacg ctgcgaagag gaaagcaccg ttgtcgaact cgttggtgat ggccgcgccg
     4621 gccaagccgg ctcccagcgt gttggcatcc cagccaccga ggaccttgcc cgagacgtcg
     4681 tcaatggtga ggcccggacg cgaggtcccc gtgggccaga cgctgtagac cgcgccggcg
     4741 ttctggggaa agccgacctt ccagccgtcc tggtcgagtt cgatcgtgac caccagcggc
     4801 ttggcagggg tgatcgaatg cacgttcgtc aattcgaaga ccacgggcgt ttcgaggccg
     4861 ttggccccct cgtcactgcg ggcctccggg tggttggagt ttaccacgcg gatgctgccc
     4921 ttcacggtga tgttctcggt aggcgaatcg acgtatccgc tgtagaacag gtttacgtag
     4981 aggccgcctt tcgtgttccg gtaaagctcg ttccattgat tattctctgc gtttgcggag
     5041 atgacaccga gttcgtggaa gcattccgcg ccgtcagagt tcggcgcgcc gctggcaccg
     5101 ccggcggccg taagctgaac gtagttgatc acccactcga tacggatgcc atcctcgttc
     5161 atgaagggaa attcacccgc ggacttggag gcgattcccg tccatgcgta ccagcccgag
     5221 ttgttaagaa tcttcaggga actgtccgac tccgatggag ctcctttggc ctggtatggt
     5281 acataccagc cgccgccgac gccgttcacg ttcgttccaa cggcgttgtc atcgaaatgg
     5341 tcgttgacgt acatgccgcc cacaatgacc aggctcgact ggatctggat gctgtcgccg
     5401 gccagccagc cggacgcggc attcggcgtg gcggttaaca ttgttgtgcc gtagcctccg
     5461 ctcaccggac ttaccctcag cactgcactg gtggtgccgt caggaatggg atacgccacg
     5521 cccggcgtga gcgtcaggct cagcgccggc accgtcaccg tgatgatcgc cggattgtag
     5581 gagaacgtga gctcgagccc gctggaactc tggcccgaag gggccgacag ggtgacgtcc
     5641 accggagtca ggttcgtggg gtcaaggaat tcgatctcat gaatgtcgaa ggtcatggcg
     5701 accgaggaat agtgggcggc gatccgctcg ggcgacaggg ccgcgtcgta gacagccact
     5761 tcgtcgatct tgcccgggaa gccccagtca ccctgcgggt caatggcgcc gatctggaag
     5821 ttgcccagga aaccatcggg cggcagcagt tggaagtcag cctggttgat gacctgcaga
     5881 ccgttgacgt aataatccaa cttgccggca ttgaacaccg ctaccacatg gaaccagtgt
     5941 tcgtcctcgg gcaacccgcc gggaatgggc ccgttcacgt accattccgg gacgttctcg
     6001 acgcctatgg tcgcatcgct ggttcgcatt tcgagggcat aatagggatg ccagtaatcg
     6061 tcggcgcacg acgcgatacc gggtcggtag ttgccgaacg gccaactgaa gttgtcgctc
     6121 cgatacttga tgcccggcgc cggggcgacc caggcctcca ctgtgcccgt gccatcggcg
     6181 aacgtgaagt cctcgacagc acccatactc accatgccgc cgctgctgcc gctgttgtcg
     6241 aaagccaggg cctgtccgcc gccgaaaccc gaggtccagc gcagcttggc gggctcgacc
     6301 agcgtgccat ggcgaccgtt acccgagttg tccgtcacgg agatggtgtc gccttcgaag
     6361 ctgtagtagc ctatcggagc atcagccatg acttgggccc cgtaggccgc ccggctgggc
     6421 agcggcgttg tgggcggggc caccccgacc gccgccaggt aatgctgggc aatggcagag
     6481 tcgctcaacc aggtcgagta gacggctact tcgtcgatca ggccgatgaa agcactgccg
     6541 ccgttcgggt cggctgcacc gatctggaac ggcgcgtcgg ttttcgcacc gaggtatccc
     6601 cagaagtcgc ccagcaagat gccgttgacg aagacccggg tcgtgggata gttcgtgttg
     6661 ttctggtagt taggggtaaa cacgaacgca acgtgatacc actggtccgg ccggaagttc
     6721 cagtactccg ggtgatagcc cataatcccc ccgaaaacgc cccagccgag tctggtcttt
     6781 tcgaggtgca tactccagcg ggtataaggg ttgggatcat tgcccggcgt gacctcgcgc
     6841 acggacgcca gcgccggatt gtagctcggc ggcgtcaacg gatcccagct cgggcgaacc
     6901 cacatctcca ccgtgccggg cgtggccaac tccgctgact gaccaccgaa ggcgaattcc
     6961 gcaaccgcgc cgaagttgac ccagcccgcc cccgtaagcg acaatgcttt gccggctccg
     7021 ccgaagccct caccggtcgg ggtgaaggtc gccgtccctt gcagcgcgcc ggtggggttg
     7081 ggtaaggccg tcgtgcaagt atccgctgcg gcggttcccg agtccgcgtc gaaggtgaag
     7141 tagctgatca agctcggctc ggcatggacc gcgtcctgat actcgctcaa gccggcgaag
     7201 gccggcggca atcccagaat ggcaatgagt gtcaccgctg caagcagatt cctgcaaatc
     7261 atcacagacc ctccatacaa agggaagcgg gaggagaaac cgcttcgtac acaactgctc
     7321 ggttcttgtt atcgatcaaa acccgcgaca ccgagagatg gagcgaattc ggccttgcca
     7381 ccggccggcc accgtgattc tcgcgcggtt ttcggacgcc cctctcccgt tccctccata
     7441 agtaaaccgg gaatgcggat cttctatccc gtactctaac aagcatccgt cgaaagcaac
     7501 atacccgttt gcgacagtcc attgcctttt tgcgtcatcg cgttcagcgg gaggcgcatg
     7561 actactcgcg gtggttcgac ctattggcgg ccgtgcgccg gaagagtgac ggctgatatc
     7621 ctgtcacctg tttgaagagc accgtcaggc gcgcagcgct cccgataccg cactgctggg
     7681 caatgaccga tagcttcagg tccgtttcgg cgaggagatg cttggctttt tcgattctca
     7741 tcctccggat cttctccagg atggaatcgc cgactgcggc ccggaaccgc cgttccagcg
     7801 tccgacggca ggttccgatt gcctcgacga ccccgtccac gctgatgtcc tccgagaggt
     7861 ggtcgcggat ccagatgagc gcggcggcca cgtcggggtc ttccaccgct gtggtttcgg
     7921 tggattggcg ggcaaccacg tgggtcggtg gaatgctcag gcgcaattgc ctgggagatt
     7981 cgcccgagag cagccggtcc agcagtctcg ccgcttcata tccgatctgc actccgggat
     8041 tgtgtacgct ggacaacggc ggatgacaca gccggcattc gaattcgtcg tcgtcaaccc
     8101 ccacgaccgc gacttcatcc gggacccgaa ggcccagttg atggcacgtg tccaccaagt
     8161 cccggccggg cttgtcgtgg gatgccagaa ccccaacagg cttgggcagc ctgagcagcc
     8221 agctttggac cgaccggctg agtccttgcc agcttgcgtc cagaaccgga acaggtcggt
     8281 actccgcgtg acacacgaag ggcgtgaacc ctgcctgggc cagacgtttg ctgaagcccg
     8341 cagcccgccc catggaacat cctccgtcgg cgcttcccag aaaaccgaag tttcggaatc
     8401 cgcggtcgag aaggtgttcg gctgccaaac gtccaacttc gaggtgatcc acgtccacca
     8461 gcgggccttt ccaggtggtg atcatactcg atgtgttcac gaccggcaca tggcagcagg
     8521 ccagcctccg cgcaacgtcc tcgtcgaaca cgctacagat cacaccgtcg ggcttccaca
     8581 tccgcatggg gcggagggct cgaatgtctg cacgggcgtc atgaaaaacc caacctcggg
     8641 tgatggcgta gtcgtggacc ccctgcaaaa cccgacggca aaaaccgatg tcctggccga
     8701 tcaacagtgc aactcgacga gctcgggtca tgaatggatt ccccagacac tccattccgg
     8761 cacgtatcgc agaagacagc agcagagaca accgaattca cctgacgcga atggaggtgc
     8821 tactatactg tatccgccat tatcggtctc gcccaggatc atgtccagcg cggaatcgtc
     8881 aagttcctcc aaattccttg acaagccgga gtagttatca gctccgtggt gcaaatgcgc
     8941 aaccaaggcg acttcacata cggcttgaat ggattccgcg atcgtcgtcc aaggctgtca
     9001 ccagcagtgt cagaaggtat cagccctgat tcaccgcaca cccaaaacga ggcaatgcag
     9061 gaacacggtg agcctgtggc aaaaccggcg ggagacttcc agcgcggatt ctcggttgag
     9121 agaggtgtga aaatgccaag aagtccgccg taagcctgcc ctcgatgctg catggcagag
     9181 gtccggcgca aaccgacaag gcttttgcgc aaaatgatat tcacgagccg ccgatactac
     9241 cgctatcatg cggtacaggc agcaggcatg aggcagcgat cgccttgggt gctgccgcga
     9301 gggcagcgag ggatcgtttg ctgcatgcca acgcagtttc ggagatctca aaggatgccg
     9361 tgtccaagaa cgatgtcggt ggacagcttg gttgtacggc gaggggcaag gccggctcgc
     9421 cggggcggga acccggttcg gcagccgggt ttcacgctga tcgaagtgct cgtggtggtc
     9481 tcgatcatcg cgttgctgat agcgatcctg ctcccgtcgc tgtccaaagc tcgcgaacag
     9541 tcgagacggg tggtctgcca gaataatctg aggcaactcc agcatgccat tgttttctac
     9601 ctttcggacc acaagggcat attccctccg caccgcacca aggtccaggc aggcacaaag
     9661 ggccgggacg acctcggaga gtgggcgtgg tttcagcagt tggagcgata cacgaagtct
     9721 ccggagatac ctcactgtcc gacgctggca aaccacacgc aagaagactc gggtattgtc
     9781 tggtcatggg cctacaaccg gctggacatc ggatacggtt acaacgcgtg gttccttggg
     9841 ctttggaatc atgcgaccaa tggcggctat gaagaatatg ccggccttcg gagtcatccg
     9901 tggttttcgg agagtcgcgt caaaagcccg tctctcaata ttctgctcgc ggatgccaat
     9961 ccgaagaatg acaggttgtt cggtgggcag ttgtggtggc ctttcgtcga cggcgacacc
    10021 ggcgggacgg gcgagggtgt caacgtcagg agacatcaga aaggcggcaa catcgttttc
    10081 aacgacggcc acacggagta tcgcagggcg ggcaccatca acccgccggc aggttcgccg
    10141 aacaaattcc tgcagtattg ggacccgctt ctacgacgcg cgaaacagtg atagtgtcac
    10201 ggagtcccgg cttgacaggt gaaatgctca ggatgaggtg acctatccgc agaggggagg
    10261 tgcaaagaaa ctcgcggggg cacaaagtcc gctaaaccat cgcggctggg gttcaggttg
    10321 cctcgcggga caacgccgga atacaatggg aaatgtgagg attggcgaga gcggtcggcc
    10381 gtcgtcggcc ggcaggtctt acgcttggat tagagaacac aatgaatgga ggagaaaacg
    10441 tgaaaaattc tatctggttt gtttcagttc tggcagtgct tgtggcggcg ggtgccgcca
    10501 atgccgtgcc caacgtcccg ccgggcgtta ccggtctgtg gcggttcgaa gccagggacc
    10561 cggtcacaca gcgggcgaca atgaaagcga ccattggcgt cgacatgacg agcagccacc
    10621 ccgacaacag cgcatggatg gtaggtcctt ggaccgttat tgagcccggg ctcagcgaca
    10681 acggcatcgc tcaggagcgc agttgggact accttacggt caaccccagc ttcaccccca
    10741 acggcggcgg cagctatgtg aatgagtaca ccgttgccat tgattacgtg cagaccagcg
    10801 gcctcaccgg ctggaacagc ttgttccaga cctcatgggg cggcaacgat agtgacggcg
    10861 acctgtttac cgacggcgcc ggtcacatcg gtatcggtgc agccggctac tcgacactga
    10921 cttatgatgc gtcgaaatgg cacaggatcg tgtggtccat cgacaacggc agcttcttcc
    10981 gggtctatgt tgacggcgtc ctcttcctcg acggcacacc ccagccgatc gatggcaggt
    11041 tctctctgta tcccgacagg ttccatctct tcgcggacga cagttgggaa gatggttggg
    11101 gcctcgtggg tacagtggcg gtgtggaatc gcgctctgac cagcgccgaa gttgctggaa
    11161 tgggcggatg gatcgacggt gccgatatgc cgacgccgct ggagattccg gaaccggcca
    11221 cgctgggttt gctgctgggc ggccttccgc tgctgcgacg tcgtcgggca tgaagctgac
    11281 atcgccgcac cttctgttca ggttcgaccg gcttggagcg gcgcctggat caggaaaagg
    11341 ttagtctgtt cgtgccgggc gggagcgttc ggctgccgcc cggcactttg tttgcgcgaa
    11401 gtgccggcgt ctccgcgcgg agagtgttcc tcgtgcagcg tattggccgc ctgacgatat
    11461 cgatctggac tgcctgcctc tgtctcatat ggtcggccgg acccgttgcc gcgcggaccc
    11521 tcggccagtg ggacttcgac gggggcgacc tctctgccac aagtggcgtg gcgatggagt
    11581 actacggcgg cacgacggcc tccaagaccc agttcggcac aaccgtctcg ttcggcattc
    11641 ccaatatcgc cggctcggtt gcgtcggtga tgaagttccc cgcttgcctg cccagtgaag
    11701 gttatgtcat gcacaccaac gcccccggca acggcggcgg catctacatc aaccaatact
    11761 cgctcgtgct ggacctgctg tacccggccg ccagcacgaa tgcctggcgg gcgatcctcc
    11821 agacctcacg caccaacggt aacgatgccg acttcttcgt gggcaacggc acgacgaccc
    11881 cgaatgcaaa cggcatcgga gtcgccggcc aataccacgg cgcgatccag ccaaacacgt
    11941 ggtatcgcgt ggcggtcacg gtcgatctca ccacgctgac gatggccaag tacatcaacg
    12001 gctccctcgt cggggtccag tcgctcgcgg actcgctgga tggccggtgg tccctctact
    12061 ccaccaacac tgcccccgat tggctgctcc tgttcgcaga caacgacaat gacacgaaga
    12121 ccggctatgt gaacagcatt cagttccgcg actacgtcat gtccgccccc gagatcgccg
    12181 agctcggcgg ccccagtgct aatggcatac cccttccggt ggccccgacg aacctgcaac
    12241 tggtgagccc caacggcggc gagtactggc cggccggctc cacacaggtc gtctcctgga
    12301 ccgtgacgga cccgagcggc gtcgtcaacg tcgaactgta cgacggggcc tcgctacggg
    12361 ccccactggg acaggcgccc atggttgacg gtcggttcga ctggtccatc tcgccgtacc
    12421 tgggtgattc tgcagattat cgcgtgaagc tttccgcagc cgcgtttccc gccgtctcgg
    12481 acgcctccga cgcacccttc gaagtctatg gatccgcgcc cgaacccacg gtcatcacca
    12541 agttgccgat gctgcaagac gcacgcccgg acgcgatgaa tctcatctgg gaaacgagca
    12601 gcttcgggaa ccccaatcgg gtcgatttcg gcctgaacga cgtgtcagag aacacgatcg
    12661 ccaacgtgtc gactcagcag ctcgatgcca cccacttcgt acacgcggcc acgattcagc
    12721 ccctgcagat cgagacggta tacaagtacc gcgtccgcag cgggactgcc acgtcaccga
    12781 cgttcacgct gcgcactgcc ccacggcccg gcactcccat caggatagtc tggttcgccg
    12841 atgagcagga ctacacgatc ttccggcaac aggttccgca catggccgca cgcaagcccg
    12901 acctcgtgct ggcggccggt gatctgatga acgacggggc aagtatcgcc cagtggcagg
    12961 attattggtt cggcccgctg caactgggca atctggccca gaccacgccc gtcctgttct
    13021 gcagaggcaa tcacgacggt gaaggcgctc tggcctacgc ctacagcgcg cttccgggca
    13081 acgaggcctg gttcgccttc acatatggaa gcagccgctt catcttcctc gatacgaaca
    13141 ttgagaccgc cgagcagact gcctggctcg aaaatgaact cgcctcaacg gaggctcaac
    13201 gagcttcatt ccgcgtggtt agctttcaca agcctcccta caccgacttg tgggacggtc
    13261 ccggatacaa cggcgaggct ttcgtccggg agaactgggt gccgctattc gaacagtata
    13321 aggtcgacgt cgtcgtgagc ggtcacacgc acgcctacct gcgcggcatg aacgacaatg
    13381 tgatgtacgt aatcgtcggc ggcgccggta acgtcgtcga cacctacacc ggctatgtct
    13441 ggggcttctt cacggtgaaa cagtcactgc accactacgg cgtgatggag gttgacggct
    13501 atgccctgac ctggaatgcc tacgacgtga acgacgtgct ctttgattcg tacgggctcg
    13561 ccagccaaac gccgttctgc ccggccgact tcgacggtga cacggacgtc gatgaagacg
    13621 acttcgtcct cttccggccg tgcttgagcg ggccgcatgc ccctcccgca gcggactgct
    13681 cgcaggaaga catcgacgcg gacggcgacg tcgaccagac ggactacggc ctgtttcagc
    13741 ggtgttacag cggaacgaac gaagttgccg ccgcaaactg cgcgcagtga gcggcgcggt
    13801 caccgttatt cccggatggt cggaaattcg gtgatcctca gccgggtgca gccataaggg
    13861 atgagttcca gatcgactat cggctcgttt gagaacacgg gactcacagg cggatcgccc
    13921 gcggagttgt gctccaggtc ccagccgggg atggccctgc ccctgacttt cagcgtcacg
    13981 ggtgcgacgc cctgcgcgaa ggggatcttg ccgacggtcc gggtaacaac ggagaccgat
    14041 tgctcggggc tgttgcgatc gagaagcagg ccatagttcc agggcgtcgt cggatagacc
    14101 gcccagtcgt tgacaggaaa cttgtcatga tgcgatctca gcctctcata tcgctcgccg
    14161 attttgagcg agaagcagag cggccccctg aggatcgcga ccgagttgtt gtatcgggtc
    14221 tcggtgcgca gcttcatcgg cagcgaaatc gtaaccgcct cgccagggtc ccacgtcctg
    14281 tctacgacgg cgaatcgccc tgcttccact ggggtctccg tgcctccaca tgcgacccgg
    14341 gcacccgtcg cccagacagg aatgcgcaga tgaagcggaa accgggtggg gcgagacgat
    14401 tcgaatacga gtcgaacgac cccgtcaaac ggatacgcgg tttcctcgga aatgctcacc
    14461 ttctcgccct cggccacctt ggccgtgacc ttgcacggtc cgtaggcgac tgcggcaagt
    14521 ccctgatcgt gtgtggccat ccaaaggtgc gacacgaatt tcggccagcc ttggtgcatg
    14581 ttggccgtgc agcatccgta gttcggctcc agaccgtata tgttcgatgt gtcgtcgttc
    14641 ccgtggctcc attgccgctt ggcgattgta cacagcacct ggttggcctg ctggtcgaac
    14701 tgatgagccc agtaatccgg catgcacgta ccggggttgg cgttgtacgc cagtcgctcg
    14761 agtcgatcgg cgaaggcaac gtccccgagg acctcgacca gcttctccat ggagaacata
    14821 tactcgacaa cgccgcacaa ctccgtgccc tgcgtcggtc gctttcccga caggtgctcg
    14881 tcaccgccga acccgccggc gacctgcccg tgatgctcat ccaggcttgc aatgccccga
    14941 aacgtcgcct gccggtcgcg ctcgtcgccg gatcgcaggt accacagagc cgggtttttg
    15001 accgccatgg cgttgttgac atcatgcgtg aagtgtgtgt atttgagccc ctgcttcagg
    15061 gcctctgtag tataggggaa gtccaggaaa aacggtgtcc agtcaaagca gctcgcgtga
    15121 atcgagtctg ctgccttgag aatctccgcg tcgccggttc ggttgtacaa ccagaatgcg
    15181 ctgacaacat tctccatggc cctcactccg cgccattcgg tgtcgggcca gtcgggcggc
    15241 gtgcggttga tgtatttgaa gtagtttgta agcaatgcga tcac
//
LOCUS       DAOTPW010000133        15280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3841_length_15280_cov_4.990345, whole genome shotgun sequence.
ACCESSION   DAOTPW010000133 DAOTPW010000000
VERSION     DAOTPW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15280
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3841_length_15280_cov_4.990345"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1344
                     /locus_tag="P5316_12860"
     CDS             139..1344
                     /locus_tag="P5316_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRR85892.1"
                     /translation="MPWFEYEGLTPGRTAIAGRVEAAHRERALEDLAQMRIEVRELRV
                     AEPPPRGRAAISEDDLVFFNEQLASLAEAGIALDDGLAQMARDVESPRLREWINALVT
                     DLRSGIPLDRAIAAREQGLPLLYSRVIRAGIDSGELPATLLNLNQHLRLAGNVRRLVW
                     EVVSYPLLVACIALTVISAFFLLVVPQMREIFADFGTALPALTVLLLQIADHYFLLLI
                     GTGCAAAVLAATWTSLRFSESGLSLREGIVMSIPVIGRVYRASLIARFLRCVSTAVAT
                     GIPLPQAMRLGGGATGSVRLAADAERLAAEAEKGRSVFVASQTARIIPPLFGFCVQVA
                     VGRDALPTAIGQLARSYENRALHAQSMLRTLLFPLLVIVLGGFLALAIVGMFLPLVTL
                     INSVGAGGA"
     gene            1354..2511
                     /locus_tag="P5316_12865"
     CDS             1354..2511
                     /locus_tag="P5316_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRR85893.1"
                     /translation="MIPELIILLSVALGCVAAIFLLWQAGTYVVAWFRRPAVERIVIS
                     QIAGVLRQNLPLPMALSLAGDSEQGMARTHLKRISKLLSQGASLSEAMELGYRECSSL
                     VVSLVAAGEKAGRLPAAVEQAEEYLIRKARWRRLFDVPLGLYVVLVLSLAGLMVSGIM
                     VAIIPKFKTIFADFGTKLPEGTVTLIDISEWFVAGTPPGWLLVFLVAVAWIVLRFWKI
                     EGQSVASRIGEAVRSNLPGVRRVEFGRGLAEMLRLMRTAVAGGMDLATAARLASSLRV
                     NRGLCDRMRWFADLLEAGSKPADACRVCELGPVLSTALAAGTRSGNMDAALRYAADYH
                     GALLSRWWIMLNSVAWPCCTVGLGLIVAFVVRALFEPLVELIRSVCGGEMG"
     gene            2511..2900
                     /locus_tag="P5316_12870"
     CDS             2511..2900
                     /locus_tag="P5316_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85894.1"
                     /translation="MRSHNMTIRLRGFSVIEVTVGIVLLGMIVALSSQAIFSYQRTRN
                     ELIDRQALIWAASAQLERIAAGAALDSRPPGGFLPEGVSLYAEAEPGRGDWSGFTRVT
                     VTATATVAGGKKLREQISRFIPAEDKP"
     gene            2897..3400
                     /locus_tag="P5316_12875"
     CDS             2897..3400
                     /locus_tag="P5316_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85895.1"
                     /translation="MRNRCRKGFTLIQMVALFPLLLVILAIGLQAERRIVQAQVFESR
                     MLSSQAMMRDVVRRFQADARFADEAVVRRGGEGIALELARGGDRIIYRCVKNRVERTE
                     HSAGADPIRYTWDLERVLADIKHESIGDSRGVVWLLFDCQLPMGDGFYVDRHLAMAVR
                     VGEGGAS"
     gene            3397..3852
                     /locus_tag="P5316_12880"
     CDS             3397..3852
                     /locus_tag="P5316_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85896.1"
                     /translation="MKGPASRSGFALVVVVVLTALTAGLLSMLTLWANYRYREVQSER
                     LRQAAHAVSDSAVAYARSHLSEWKSNPPVDRIVLEVDSLVPAGMSASASVVPVTEGGR
                     RVCRISSRVEGGALSSGDTVDLPLGPAPTTSSTATSFTRVREEAPPETP"
     gene            complement(3919..4248)
                     /gene="trxA"
                     /locus_tag="P5316_12885"
     CDS             complement(3919..4248)
                     /gene="trxA"
                     /locus_tag="P5316_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRR85897.1"
                     /translation="MAGANTKTFTEDNFQQEVLEATEPVLVDFWADWCMPCKALGPTI
                     DELANENVGKAKVGKVDTDANPSLATRYAISAIPTVILFKNGEVKERFVGLRSKRDLQ
                     ASLDAAM"
     gene            4475..6097
                     /locus_tag="P5316_12890"
     CDS             4475..6097
                     /locus_tag="P5316_12890"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR85898.1"
                     /translation="MRLHERKYLGVLILTTVALDLLSPACCRADAFVRIQGDQFVLNG
                     QVYKLKGTNYYPQNHMWADMWNSWDWPEITSEVDRMRDLGLNCVRILVPYSRGGWNGP
                     DIPADRLQKLEDIVNLMGDHGIRAVVTLFDWETSFPASGSSTWNNHLTHMWKIVDRLK
                     NNPFVLMWDVKNEPDHPSNYGSCDCNPGACGDWDCNPSSRDKIVSWLERMCNAVRSRD
                     TNHPVSAGMRWWRNLPDVLSFIDVAIFHSYWPNIGTEEIPETKGLLGSNQKPILVEEW
                     GWPTNPTPCNRDGQLIHDYNEAHQYSVYVNHLTAFEEHNIAGGLQWMTFDAKPYTTDP
                     NESFENYFGLWKYGYALKPAGIYYREHYPVMRFPGTPPGTVTHLRLLRSGRGVEVSWT
                     NPADGDFAGTMIRFAVAAAPALPTEGILLCDCPGAAGSSDSFIQSDPPRGLVFYSAFA
                     YDTGVPSYAAPVSASIWVSAPGDFDGDGDVDQVDFGHLQTCFSGPFVPQTNPNCRNAM
                     LDYDNDVDNGDFAIFQACFSGSDVAADASCGD"
     gene            complement(6211..7140)
                     /locus_tag="P5316_12895"
     CDS             complement(6211..7140)
                     /locus_tag="P5316_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR85899.1"
                     /translation="MRKGIIALLCCGFVLASSAMAAPVYVKTWDFEDGTVGQPHPDWN
                     AGTVQKNGPYSGTPYPKGNRLYTGDNVASWHTLAQPTNSFIYTTQMWTDAGNINGIRK
                     AGMGYRYAGTTHEVWFEGDDQGGWPANAPSRTRFGDSWLPNPRPGTEDQWDNYQWQGG
                     GGRRLERSTLDGATNLGSHYLPSLNTPGVPDWEFPQFAEIELMIKYNTPDNPGTVELW
                     FRSLNYNATRSAQDQWLRLGWGGYAGANYYDFILGINPATNDYWEIDRIKLGGGSAWS
                     QAAYDNVYFQSIPEPTTLGLLLLGAVPTLRRRR"
     gene            complement(8013..9881)
                     /locus_tag="P5316_12900"
     CDS             complement(8013..9881)
                     /locus_tag="P5316_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85900.1"
                     /translation="MQLGFLAKAKVLVVGCSVLYLVVPAWGQESWTYVDSSGFKAFDT
                     PWPATNGVLNRPLSNYHTWGDIRRVKVNSMVVDLEGRVYAACTNLNNNGSSPGGVTIF
                     NTDGSRIDIDLSPATFGSGVDENLPGAVTKLVLAGDGKVYGLQNWLEVSFAYAAGTVS
                     RILRFNADGSVDVIKEYSPLVTSTNGEWVNKIGGLTVGGDGYPYWWFAGATWGTGNAY
                     WKQHVLWRYNLVTAQVEEFPLASLDPQGTDGAEQPNRMLNFEYVGTGIGSDFASYFTV
                     LGGLGGARWVADAIRWNAHRSFDVPGAVADAGWGRDWCTATAYDKVSRRLWAGGRGTG
                     YADPDPVKQGTNIMTMWWNDMIGDLGLYHRDNEGFLSMSGNLSWHVNDNDPQTSGVGN
                     GGPYWITALAANSGGSAWLSIGGQIANSKKPGDGNYTFAGPWGLVGEVYKMEVDTSST
                     PVLTRTDMGRPHSAHPTDSINENQVVALAFGLDKVYALVLDLVNGAYSLYVEDNPDKP
                     FKGACCMPSGCIEVAQNQCPGEFQGIGTDCDSINCSCRTCHDPFADADGDGDVDQTDF
                     AVIQMCFTGQDGGVPNAPCRCDCYNRDNDEDVDANDLALWMLCASGPGIPADKDCD"
     gene            10539..11660
                     /locus_tag="P5316_12905"
     CDS             10539..11660
                     /locus_tag="P5316_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRR85901.1"
                     /translation="MSEPHADVRVSHRPAPRTTLAHVAERAGVSIATASLVLSGREEY
                     IKQFRADTVEKVRRCAERLGYRANLFAAGLPTKTSLFFLLVIRDFGGSQDPSSWHLWA
                     FEGMLLAGAMRTATDEGLYPVVAAIDPSDPEAGIATVERIIGGGVLGAVVRTPNPPLE
                     RLIRSRLKAAQRLVVVFPHKLSAWPTNAIDVDNRAIGETAGRLLISRGRKRLALVCYK
                     SIRDIHRLRLEGFKRAAEEAGATVRIIQLPLAVNEQSAAGTVASRMKRVAADGLFAVD
                     SVSSVGSLLGLVRCGMKPTEDFDLVGCDCSLWRNEPMPAITTVDVSWEEVGILATKKL
                     LEMSQAGTSRFKTIVLPPRVTPAGTCPVPDGFHPVSASK"
     gene            12024..>15280
                     /locus_tag="P5316_12910"
     CDS             12024..>15280
                     /locus_tag="P5316_12910"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HRR85902.1"
                     /translation="MARSVVRTFGAAVVTLGVWLSGCLSVGPGSVYAQCPPYWDRLGT
                     GTTAAIYALAEYDGAIYAGGWFTTAGGQEAYCIAKWDGTSWSALDAGMNGYVYALAVY
                     DGALYAGGSFTRAGGSTANRIARWNGTAWSAVGSGTNNTINALAVYNGVLYAGGSFTT
                     AGGSAANRMAAWDGFTWSAVGSGVDNTVSALASYAGVLYVGGSFTTAGGAAANRIAGW
                     DGVSWFALDSGVGNTVSALAVHNGLLYVGGAFTTAGGAAASRIATWNGAAWSTVGSGV
                     NNTVSALVSYDGVLYAGGSFTTAGGAAANRIATWDGVVWSPVGSGVSSTVRALAVHNG
                     ILYVGGSFTSAGGQIAYYIATWNGPGWTWSAVGGGVSANGTSSAVYALTLYNDVLYVG
                     GQFTTAGSLTANRVARWNGTTWAAVGSGTNGNVNALAVYDGALYAAGSFTTAGGQAAN
                     NIAKWNGSIWSAVGSGVDNAVSALAVSNGVLYVGGAFTTAGGAPADYIAAWDGASWSA
                     VGSGVNNAVSVLADYDGVLYAGGAFTMAGGAPASYIAAWDGAGWTAVGGGVNNTVGAL
                     AVHDGALYVGGTFTTAGGSAANRIARWNGIAWSALGSGVNNAVVALGVYGGALYAGGS
                     FTSAGGSAAARIAKWNGTAWSALGSGLNSTVRALAAYDGVLYAGGSFSTAGGWPANYI
                     ASWAEHTVSFAAGGNGSLVGDTLQVVGQDCSTTSVTAVPNVGYHFTGWTGDYVGSANP
                     LTLTNVTGDMNITAQFAINTYTVTFAAGAHGSLAGTVMQTVNHGDNCSAVTAVPNANC
                     NFTGWTGDYVGSDNPLTITNVTADMTVTANFASDTYTVTFLAGPQGSLLGTTVQTVDH
                     GANCTAVTAVPSAGHRFAGWTGDYIGNDNPLTVTHVTANMTITANFGSRIHTVTFEAG
                     EHGSLQGDTRQEVDYLADCTAVTAVADPGYYFIGWTGDYVGNDNPLAITSVSTDMVIS
                     ANFADHPPPSNVPPAPRLVVEIASNEPGNNVVCKMDDELSFLLHVGNIGDRDATDVRV
                     VISLPENVEFVNARVRDDIAARGTPENARLADLQVVLTIGSLGAGETVVIDMVLRV"
BASE COUNT         2809 a         4454 c         4913 g         3104 t
ORIGIN      
        1 gaaggctgat cgagcaaggg cggacgacgg ttgatgaggc tcgacgcgtg ctcggatcgc
       61 ttttcacgtg caacgaatct gaaaaaaggt cgccggtgcg acactgacag cgggccggcg
      121 cgacggggag aacaagaaat gccctggttt gaatacgaag gtttgacgcc cggcagaacg
      181 gccattgccg gacgtgtgga ggccgctcat cgcgagcggg cccttgagga tcttgctcag
      241 atgcggatcg aggtgcggga actgcgcgtt gccgagcccc cgccgcgagg ccgtgcggcg
      301 atcagtgagg acgatcttgt ctttttcaac gagcaactgg cctcgctggc ggaggcgggc
      361 attgctctgg acgacgggct ggctcaaatg gctcgggatg tggaatcgcc ccggctgcgg
      421 gagtggatca acgccctggt gaccgacctg cgaagcggca tcccgcttga ccgggccatc
      481 gccgcgcgcg agcagggttt gccgcttctc tacagtcggg tgattcgggc cggcatcgac
      541 tcgggcgagc tgccggccac gttgctgaac ctcaatcagc acctgcggct ggccggcaat
      601 gttcggcgac tggtgtggga ggtcgtcagt tatcctctgc tcgttgcgtg cattgccctg
      661 acggtcatct cggcattctt tctgcttgtt gtaccgcaga tgagagaaat ctttgctgat
      721 ttcgggaccg cgctgcccgc ccttaccgtt ctcctgctcc agattgccga ccattatttc
      781 ctcttactca taggaaccgg ctgcgcggcg gcagtccttg cggcgacgtg gacgagcctg
      841 aggttctccg agagcggcct gtcgcttcgt gaaggtatcg tgatgtccat tcctgtcatt
      901 ggacgcgtat accgagcgtc tctcatcgca cgattccttc gctgcgtttc gaccgcggtg
      961 gccacgggta ttcctcttcc ccaggcgatg cgtcttggtg gcggtgcaac cggttcggtg
     1021 cgcctggcag cggatgcgga gcgtctggcg gccgaagcgg agaaggggcg gtcggtcttc
     1081 gtcgccagcc agacggcccg catcataccg ccgttgttcg gtttctgcgt ccaggtggcg
     1141 gtgggccggg acgcgctgcc aaccgcgatc ggccagctcg ccaggtcata cgagaaccgt
     1201 gccctgcatg ctcagtcgat gcttcgcacc ctgctgtttc cgctgctggt cattgtgtta
     1261 ggagggttcc tggcgttggc gatcgtgggc atgtttcttc cactggtgac attgatcaac
     1321 tccgtcgggg ccggcggcgc ttgaggtgag aatgtgatac cggaactgat catcctgttg
     1381 agcgtagcac tcggatgcgt cgccgcgatc ttcctactgt ggcaggcggg gacgtacgtt
     1441 gtggcctggt tccggcggcc ggcggtggag cgaatcgtga tcagccagat cgccggcgtg
     1501 ttgcgtcaga atctgccgct accgatggcg ctgtcgctgg ccggcgattc cgagcagggc
     1561 atggcccgca ctcacctcaa gcgaatctcc aagcttctgt cacagggagc ctcgctgtca
     1621 gaggcaatgg aactgggtta tcgcgagtgc tcatcgctgg tcgtgagcct ggtggcggcg
     1681 ggagagaagg ccggccgact gccggcggcg gtggagcagg ccgaggaata cctgatcaga
     1741 aaggcgcgtt ggcgacggct gttcgatgtg ccgttgggac tctatgttgt gcttgttctc
     1801 tcgctcgccg gactcatggt cagtggaatc atggtggcca tcatccccaa gttcaagacc
     1861 atattcgccg atttcggaac caagctgccc gaaggtaccg tgacactgat cgacatttcg
     1921 gaatggtttg tggcgggcac gccgccaggc tggctgctcg tattcctggt tgccgttgcc
     1981 tggatcgtgc tgcggttttg gaagatcgaa ggccagtcgg tagcgtcgag aatcggtgag
     2041 gccgttcgct caaatctgcc gggggtccgg cgcgtggaat ttgggcgagg gctggcggaa
     2101 atgctgcgtc tgatgcgaac cgccgtggcc ggcgggatgg atctggcgac agccgcgcgg
     2161 cttgcttcga gtctgagagt caaccgcggt ctgtgcgacc ggatgcggtg gtttgcggat
     2221 ttgctcgaag ccggcagtaa acctgccgat gcctgccgcg tctgcgagct ggggccggtg
     2281 ttgtcgaccg ccttagccgc gggaacgagg tcgggcaaca tggacgctgc tctgcggtat
     2341 gccgccgatt atcacggggc cctattgtcg cggtggtgga tcatgctcaa ctcggtcgca
     2401 tggccctgtt gcaccgtcgg cttgggtttg atcgtcgcgt ttgtcgtgag ggctctgttt
     2461 gagccgctgg tcgagttgat ccggtcggtt tgtggcggag agatgggctg atgcgaagtc
     2521 acaacatgac aatccggctt cgagggttca gtgtcatcga ggtgacggtc ggcatcgtgc
     2581 tgctgggcat gatcgtcgct ttgtcgtctc aggcgatatt cagctatcag agaacacgta
     2641 atgagttgat tgaccggcag gcactcatct gggcggcctc ggcgcagctt gagcgaattg
     2701 ccgccggggc ggcgcttgac tcgcgtccgc cgggcgggtt cctgccggag ggcgtctcac
     2761 tgtatgccga agccgagccc ggcagaggag attggtcggg tttcacccgc gttacggtga
     2821 cggccacggc caccgtggcc ggcggaaaga aactccgcga acaaatcagc agattcatcc
     2881 ccgcggagga caagccatga ggaatcgatg ccgcaagggt ttcaccctga ttcagatggt
     2941 cgcccttttt cccctgctct tggtgatcct ggcgatcggc cttcaggctg aaaggcgaat
     3001 cgttcaggca caggtcttcg aaagccgcat gctttcgagc caggcaatga tgcgagatgt
     3061 cgttcgccgt tttcaggcgg acgcccgttt tgccgatgaa gccgtggttc gtcgcggcgg
     3121 tgaggggatt gccctcgaac tggcccgcgg cggtgacagg atcatctacc ggtgcgtgaa
     3181 gaaccgggtt gaacgaacgg agcattcggc cggcgcggac ccgatccgct atacatggga
     3241 ccttgaacgc gtgcttgccg atatcaagca cgagagtatc ggtgattcaa gaggcgtggt
     3301 ctggctcttg tttgactgcc agttgccgat gggcgacggg ttctacgtcg accggcattt
     3361 ggcgatggcc gtcagggtgg gcgaaggggg tgcctcatga aagggccggc aagtcgttct
     3421 gggttcgcac tggttgtggt cgtggtgtta acagctctga cggcgggttt gctgtccatg
     3481 ctgactctgt gggcaaacta tcggtatcgt gaggtacagt cagagcggct ccgacaggcg
     3541 gcgcacgcgg tcagtgacag cgccgtcgcc tatgccagat cacatctcag cgaatggaag
     3601 agcaatccgc ccgtggaccg cattgtgttg gaggtggatt cgctggttcc ggccggcatg
     3661 tccgcatcgg cgtcggtcgt tccggtcacg gagggcggtc gccgtgtctg ccggatttcc
     3721 agtcgggtgg aaggcggggc tctctcctcg ggcgacacgg ttgacctgcc gcttgggccc
     3781 gcgccgacaa catcatcgac ggcgacgtcg tttacccggg tcagggaaga agccccgcct
     3841 gaaaccccgt gatctgagcg cggctttgca tgtctgtttc ggggcggctc ttgccaattc
     3901 acggaggggg gcctggggct acatggcggc gtccagggag gcctgaagat cgcgcttgct
     3961 tctgaggccc acgaagcgtt ccttgacctc gccgttcttg aataggatca cggtgggaat
     4021 tgcactgatc gcgtagcggg tggccagcga cgggttggca tcggtatcga ccttaccgac
     4081 ctttgccttg ccgacgttct cattggccag ttcatcgatg gtcggcccca gggctttgca
     4141 gggcatgcac cagtcggccc agaaatcgac cagcaccggt tcggtggcct cgaggacctc
     4201 ctgctggaaa ttatcttcgg taaaggtctt ggtatttgcc ccggccatga ttgatcctcc
     4261 atgtcctttt tccggctggt tcgtcgtaaa acggtcagta gactcgttgt cggagattct
     4321 agaccccggt tgttcgagcg tcaacaactc gataagtgct cgtttcgaat tgcgggcgtg
     4381 tgagctggga tagcccctgt cggttggccg acatatactt gaggagagct cgaggggtcg
     4441 caacgatgct taagagcgat caaggtttca ggatatgaga cttcatgaac gtaagtatct
     4501 tggcgtcctg atcctgacca cggttgcgtt ggacctgttg tcgccggcgt gctgccgcgc
     4561 ggacgcgttt gtcaggatcc agggcgacca attcgtcttg aacggccaag tttacaagct
     4621 gaagggcacg aactattacc cgcaaaacca catgtgggcg gacatgtgga acagttggga
     4681 ttggccggag atcacctctg aggttgaccg gatgcgcgac ctggggttga actgcgtccg
     4741 catcctggtt ccttatagtc gcggcggctg gaacggacca gacatcccgg ccgaccggct
     4801 ccagaaactt gaggacatcg tcaacctgat gggcgaccac ggcatccgcg cggtggtgac
     4861 ccttttcgac tgggagacga gttttccggc ctccggcagc tcgacgtgga acaatcatct
     4921 cacccacatg tggaaaatcg tagaccgttt gaagaataat ccgttcgttc tgatgtggga
     4981 cgtcaagaac gagcccgacc acccgagcaa ctacggctcg tgcgactgca accccggggc
     5041 atgcggcgat tgggactgca accccagcag tcgggacaag atcgtgagct ggctggagcg
     5101 catgtgcaat gccgtgcggt caagggatac caatcacccg gtctcggcgg ggatgcgttg
     5161 gtggagaaac ctgccggatg tgctcagctt catcgatgtg gccatatttc actcttactg
     5221 gcccaatatc ggcactgagg agattccgga gaccaagggc ttgctggggt cgaaccagaa
     5281 gccgatcctg gttgaggaat ggggctggcc cacaaacccc acgccctgca accgagacgg
     5341 ccagttgatc catgactaca acgaggcgca tcaatacagc gtttacgtca accatctcac
     5401 ggcgtttgag gaacataaca tcgccggcgg cctgcagtgg atgactttcg acgcgaagcc
     5461 ttacacaacc gacccgaacg agagcttcga gaactacttc ggcctgtgga aatacggtta
     5521 cgcgttgaag cccgccggga tctactaccg cgaacactat ccggtgatgc ggttccccgg
     5581 cacgccgccc ggtacggtga cccatttacg gctgctgcgt agcggacgag gtgtcgaggt
     5641 gagctggacc aacccggcgg atggggattt tgccggaacg atgatccggt tcgcggttgc
     5701 tgccgcgccg gccctgccta cggagggcat tctgctgtgc gattgtccgg gggccgccgg
     5761 ctcatcggac agtttcattc agagcgatcc tccccgcggt ctcgtattct acagtgcgtt
     5821 cgcctatgac accggagtcc cgagctatgc ggcgccggtt tcagcatcca tctgggtatc
     5881 cgcgccgggc gatttcgacg gtgacgggga cgtcgaccag gttgatttcg ggcacctcca
     5941 gacctgcttc agcgggccgt tcgtgccgca gaccaatccc aactgccgta atgcgatgct
     6001 ggactacgac aacgatgtcg ataatgggga cttcgccatt ttccaggcat gttttagcgg
     6061 atcggacgtg gcggccgatg cgagttgtgg agattgaccg atctacgcga tcaccgtgtc
     6121 aagcccatcc gggcgcaaaa caaagggacg cgggatacca cccgcgcccc ttctcattca
     6181 ggttaaatga cccaggtttg gtctatcgcc tcagcggcgg cggcgcagcg tggggacagc
     6241 gcccaggagc agcagaccca gggtcgtcgg ctcggggatc gactggaaat aaacgttgtc
     6301 gtaggcagcc tgcgaccaag cggaaccgcc gccgagcttg atccggtcga tttcccaata
     6361 atcattggtc gccgggttga tgccgagaat aaagtcgtag tagttggcac cggcgtaacc
     6421 gccccaaccg agccggagcc actgatcctg cgcagaacga gtcgcgttgt aattcaacga
     6481 gcggaaccac aattcgaccg tgccggggtt gtccggcgta ttgtacttga tcatcagctc
     6541 gatctcggcg aactgcggga actcccagtc cggcacgccg ggggtattca acgagggcag
     6601 atagtgactg ccgaggttgg tcgcgccgtc gagcgtgctc cgttccagcc ttcggccgcc
     6661 gccgccctgc cactgatagt tgtcccattg gtcctcggtt ccgggccggg ggttcggcag
     6721 ccaggagtcg ccgaaacgcg ttcgggacgg cgcattggcg ggccaaccgc cttggtcatc
     6781 gccctcgaac cagacttcgt gggtcgtgcc tgcataccgg tagcccatac cggccttgcg
     6841 gatgccattg atgttgccgg catccgtcca catctgcgtt gtgtagatga agctgttggt
     6901 cggctgcgcc agcgtatgcc aggatgccac gttgtcgccg gtgtacaggc gattgccttt
     6961 ggggtacggc gtgccactat acggcccgtt tttctgcacc gtgcccgcgt tccagtcggg
     7021 gtgcggctgt cctaccgtcc cgtcctcgaa atcccaggtc ttcacataga ccggggcggc
     7081 catggccgag cttgcgagca cgaacccgca gcacaacagt gcaatgatgc ctttcctcat
     7141 ttggacctcc ttctcttcac aagatctgct tctgaacctt ttcgccgcca tgataacgag
     7201 aaacaacgga ttgttttcga ggaataccgc ctttcaaacg ccaaggtctg ccttgggcct
     7261 gctcgcatga acctcgcgtt aacaccacat ctgacaggaa cggatcgcct accaaccatt
     7321 tccatccgat cagcacgaca atggctcgtg ccgtgaacgc atcagcgatt ccgattgcgg
     7381 gcgattctcg gggaaagctt ttccgacctc ctcgagatgg cggtccgcca gaccggcggg
     7441 caggccattc gcctctgacg tgtaatttac ccgcaaaccg gtttcaggtc aagctctttt
     7501 tggctactaa aacattttag ttttgatgtt ccggacaggg tcgcgagcct ttgggggccg
     7561 caggttgcac gagctctgcc ggagcccata cccatacacg gatcacccgg gcgctttcgc
     7621 aggcgcccga cgccggcggc ggatgagccg gtttcacaaa acattttcgg acaagtcttt
     7681 acggcaacct tgcccatcga tgtcgggaag cctgttcgca tcttctgata agggcggctc
     7741 atccgaacct gccatcgcga tcaaggccga cggcaggccg cctgccaaga cccggccggg
     7801 aaaaccagga caccccattt ggtgaagccg cccgatcctt tgcctgggac gggcccgccg
     7861 tgccggaacc ccatggctca gatcgtgaag caagccgagc cgcccgtaga aaccgggagg
     7921 ctcggcttgg acacagacag aagggaggat gaaacttgtg ccccgtcaac cgcatgaccg
     7981 ccaaccagtg ccagggggcg actcgcgccg cttcaatcgc agtccttgtc cgctggaatg
     8041 ccgggacccg aagcgcaaag catccagagc gccaagtcat tggcatcaac gtcctcatca
     8101 ttatcgcggt tatagcaatc gcagcggcaa ggcgcattcg gtacgccgcc atcctgaccg
     8161 gtgaagcaca tctggatgac ggcaaagtcc gtctgatcga cgtcgccgtc accatcagca
     8221 tccgcaaacg ggtcgtggca agttcggcaa ctgcagttga tggagtcaca atcggttcca
     8281 ataccctgga actcgccggg gcactggttt tgagccacct cgatacatcc agaaggcatg
     8341 cagcaggcac ccttaaacgg cttgtccgga ttgtcctcga catagaggct gtaagcgccg
     8401 ttaacaagat cgagaaccag agcgtagact ttgtccagcc caaaggccag cgccacaacc
     8461 tggttttcgt taatcgagtc ggttggatgg gcgctatggg gccgtcccat gtcggtcctc
     8521 gtgaggacag gcgtgctgct tgtgtcgacc tccattttgt atacttctcc caccaagccc
     8581 catgggcctg cgaaagtgta gttgccgtca ccgggcttct tgctgttggc gatctggccg
     8641 ccgattgaaa gccaagccga gcctccggag ttggccgcca aagcggtgat ccaatagggt
     8701 ccgccatttc cgacaccgct tgtttgcgga tcgttatcgt tgacgtgcca actcaggttt
     8761 ccgctcatgc tgagaaagcc ctcgttgtcc cggtggtaaa gcccgaggtc gccaatcatg
     8821 tcgttccacc acatcgtcat gatattggtg ccctgcttga ccggatccgg atcggcgtac
     8881 ccggtccccc ggccgccggc ccaaagcctt ctgctaacct tgtcataggc cgtcgccgtg
     8941 caccagtctc gtccccagcc ggcgtcggcc actgccccgg ggacatcgaa ggagcgatgt
     9001 gcgttccagc ggatggcgtc cgcaacccac ctggcgccgc cgaggccgcc caacaccgtg
     9061 aagtaactcg cgaaatcact gccaatgccg gtgcccacgt attcgaaatt gagcattctg
     9121 ttgggttgtt cggcaccatc ggtgccttga ggatccaagg aggccagggg aaactcctcg
     9181 acctgggctg tcacaagatt gtaccgccac agaacgtgtt gcttccaata ggcattgccc
     9241 gttccccagg ttgcacctgc gaaccaccaa tagggatatc cgtcgccccc gaccgtcaaa
     9301 ccgccgattt tgttgaccca ttcgccgttg gtactcgtca caagggggct gtattccttg
     9361 atgacatcaa ccgagccgtc cgcattgaac cgcaggatcc ggctcacggt gcctgcggcg
     9421 taagcgaagc tgacctctaa ccagttttgt aacccgtata cctttccgtc accagccaag
     9481 acaagcttag taacggcgcc cggcaggttc tcgtcgacac ctgagccgaa cgtcgcgggc
     9541 gacagatcta tgtcaatgcg gctgccatcg gtattgaaaa tggtcacgcc gcccgggcta
     9601 ctgccgttgt tattgagatt cgtgcaagcg gcatacaccc ggccttcgag atcaaccacc
     9661 atgctattca cttttacccg gcgtatgtca ccccaagtgt ggtagttcga taacggccga
     9721 ttgagcacgc cgttggtcgc gggccaaggc gtatcaaagg ccttgaagcc ggagctgtcg
     9781 acataggtcc atgactcctg accccatgcc ggaacgacga gatacaagac ggaacagcca
     9841 accaccagga ctttggcttt tgccagaaaa cccaattgca tgcatcagcc ctcccacttt
     9901 atcagtgccg tcgcacttcg cgccctttgg ccgacccgat cgcctccggg tgctgtgctt
     9961 gagatgacga tccgccggct gtccaacaac aacctcagtt aggaacctta cgatgagtcg
    10021 gccgggcttc cggcctgttc cacaacagta caggttagcc gcttttgcct gttccgtcaa
    10081 gagtagatta cgttctaaaa cattttagtt aaggattggg gggcgtgtca agcgaaacca
    10141 agagcctggg aggcctgcag aatatccact ttggacaaac ctgtcacgac gcgcgggaac
    10201 atgggaccgc gccgtcatga acccttagcc gtgttcgccc aatgggttgc gcgtgttggc
    10261 ccgcgtttgt ccggaaggcc gagacgcgcg ggccggcatc gcagggccgg gcggcaagag
    10321 gcggtggaaa gcaagctacc gatgacgtcg ccgcgtagct gcccgggatc cggattcggc
    10381 cggggcaagc gggcactgcc caatcgcgcg tcaaagtgtc tataactccg gtcgccggtc
    10441 ggccggggca agcgttgatg tccggcgcct gaaacgatag aagtatggga taggagatga
    10501 gcgattggcg ggcatccgcg atcgcctggg ggtttcggat gtccgaacca catgccgatg
    10561 tgagagtcag tcaccggccc gcgccgcgga ccacgttggc tcacgtagcc gagcgggcgg
    10621 gggtctcgat cgcgaccgcc tcactggttc tctcggggcg tgaggagtat atcaagcagt
    10681 tccgagccga cacggtagag aaagtgcgtc gttgcgccga acggttgggc tatcgggcca
    10741 acctgtttgc ggccgggctg ccgaccaaga cttcgttgtt cttcttactg gtcatccggg
    10801 attttggcgg ctcacaggac cccagcagtt ggcacctctg ggcattcgaa ggcatgctgc
    10861 tcgcgggagc gatgcggacg gccacggacg agggtcttta ccccgtggta gcagccatcg
    10921 acccgagcga tccggaagcg ggtatcgcta cggtggagcg aatcatcggc gggggggtgc
    10981 ttggcgccgt cgtccggacg ccgaaccccc cgttggagcg gctgatccgc tcgcgtctga
    11041 aagcagcaca aaggctggta gttgtgttcc ctcacaagct ctcggcctgg ccgacaaacg
    11101 cgatcgacgt agataacagg gcgatcggcg aaaccgccgg acggctgctc attagtcgtg
    11161 ggaggaaaag actggctcta gtatgctaca agagcatcag ggacattcac cgcctgcggc
    11221 tggaaggctt caagcgcgcc gccgaagaag cgggggcaac agtaaggatc attcagctac
    11281 ctctggcggt gaacgaacag agcgccgccg gcaccgtggc ttcgcgaatg aaacgggtgg
    11341 cggccgacgg gctgtttgcc gtggattccg tctcttcggt gggatcgctg ctcggcctcg
    11401 tgagatgcgg gatgaagccg accgaggatt ttgatcttgt cggctgcgac tgttctttgt
    11461 ggcgcaacga acccatgccc gcgatcacca ccgtggacgt gtcatgggag gaagtgggga
    11521 tcctggcgac gaagaagttg ctcgagatgt cacaagcagg cacttcgagg ttcaagacca
    11581 tcgtgttgcc tccccgagtc acgccggcgg gtacctgtcc ggtgccggac ggttttcacc
    11641 ccgtttccgc gagcaagtga cgccaaccgg cgctcttatg cggctccgta caagtccccg
    11701 atgagtaaag acctatcaga aaccaactgc ctggcacatt ggcgaattcc ctttgaccga
    11761 cgctgcgtcc tcgcatagtc ctggaatatc gctaacaagc gggcggtcga cgcggacctt
    11821 atcggacagg tccatgactt tacttcggga attcaaccca tgacagacgt tggccgatgc
    11881 agcaaaagcc tcagtaacga ccacggaagg cagactccgt atgcgccctg ccttggctgt
    11941 tgccatggca gcgggtcgtc cgacccaatg acccatcgat cgagcgcgtc gtaggtgcgg
    12001 ggcaggtttt ttcggagcgg gtgatggcgc gtagcgttgt ccgcacgttt ggtgcggccg
    12061 ttgtaaccct gggtgtctgg ctcagcgggt gtttgtccgt tgggccgggc tcggtgtatg
    12121 cgcagtgccc gccgtactgg gacagactcg gtaccggaac gacggcagcc atctacgcat
    12181 tggcggagta tgacggggcg atctacgcag gagggtggtt cacgacggcg ggggggcaag
    12241 aggcatattg catcgccaag tgggacggga cgagctggtc cgcgctcgat gcgggaatga
    12301 acgggtacgt ctacgccttg gcggtctatg acggagcctt gtatgcgggg ggatcattca
    12361 ctcgagctgg aggatcaacg gccaaccgca tcgccagatg gaacggtacg gcctggtctg
    12421 cggtcggcag cgggacaaac aacacgatca acgcgctggc cgtctataac ggagtcttgt
    12481 acgccggggg atcgttcacc acggccgggg ggtcggccgc caaccgcatg gccgcgtggg
    12541 acggttttac ctggtccgcg gtcggcagcg gcgtggacaa cacagtaagc gccttggctt
    12601 cgtatgccgg cgtcctgtac gtgggaggat cattcaccac ggccggtggg gcggcggcca
    12661 accgcatcgc ggggtgggat ggtgtgtcct ggttcgcgct tgacagcgga gtgggcaaca
    12721 ccgtaagcgc tttggctgtc cataacggat tgttgtatgt cgggggggcg ttcaccacgg
    12781 ccggaggagc ggccgccagt cgcatcgcca cgtggaatgg cgcagcctgg tctacggttg
    12841 gcagcggcgt gaacaacacc gtgagcgcct tggtttcgta cgacggcgtc ttgtatgcag
    12901 ggggatcgtt caccacggcc ggcggggcgg cggccaatcg cattgccacg tgggacggtg
    12961 tggtctggtc gccggttggc agcggcgtga gcagcactgt ccgcgccttg gcggtgcaca
    13021 atgggatctt gtacgtgggc ggatcattca cgtcagcggg gggtcagatt gcctattaca
    13081 ttgccacatg gaacggccca gggtggactt ggtccgcagt cggtggcggc gtcagcgcca
    13141 acgggaccag tagtgccgtt tatgccctga cgttgtacaa cgacgtgtta tacgtcgggg
    13201 gccagttcac caccgccgga agcctgacag cgaaccgcgt cgcaaggtgg aacggcacaa
    13261 cttgggccgc agtgggaagc ggcacgaacg gcaacgtgaa cgctttggcg gtctatgatg
    13321 gggctttgta tgcagcgggt tctttcacca cggccggtgg ccaggcggcg aacaacatcg
    13381 ccaaatggaa cggttcgatc tggtctgcgg tcggcagcgg cgtcgacaat gcggtcagcg
    13441 ccctggcagt ttccaacggg gtcttgtatg tgggcggggc gttcactacg gccggtgggg
    13501 cacccgcgga ctacatcgcc gcttgggacg gtgcttcgtg gtccgcggtc ggcagcggag
    13561 tgaacaatgc ggtcagcgtc ttggccgact atgacggcgt tttgtacgcg gggggtgcgt
    13621 tcaccatggc gggcggggcg ccggcgagct acatcgccgc gtgggacggc gcgggctgga
    13681 cggcggtcgg cggcggcgtg aacaacacgg ttggtgcctt agccgtacac gatggagctt
    13741 tgtatgtggg cgggacgttt accacggccg gcgggtcggc ggccaaccgc atcgccagat
    13801 ggaacggcat cgcgtggtct gcgctcggca gcggcgtcaa taacgccgtg gtggccctgg
    13861 gcgtctatgg tggggcattg tacgcgggcg gatcgttcac ttccgcggga ggatcggccg
    13921 ccgcccgcat cgccaaatgg aacggcactg cctggtctgc gctggggagc ggtttgaaca
    13981 gcacggtccg cgcgttggcc gcctatgacg gggttttgta cgcgggggga tccttcagta
    14041 cggcgggcgg atggccggcc aactacatcg cctcgtgggc ggagcacacg gtttcatttg
    14101 ccgccggcgg gaacggcagc ttggtcggcg atacgttgca agtcgtaggc caggactgca
    14161 gcaccacgtc cgttaccgcg gtccccaatg tcggctacca cttcaccggt tggaccggcg
    14221 attacgtcgg gagcgccaac ccgctgacac ttacgaacgt cacgggggac atgaatatca
    14281 ccgcacagtt cgcgatcaac acgtatacag tgacgtttgc ggccggcgcg cacggttcat
    14341 tggcgggcac cgtcatgcag acggtcaacc acggcgacaa ttgcagcgca gtcactgctg
    14401 tgcccaacgc caactgcaac ttcaccggct ggaccggcga ctacgtcggc agcgacaacc
    14461 cgttgaccat cacgaacgtc accgctgaca tgaccgtcac cgccaacttc gcaagcgaca
    14521 cctacacggt gacgtttctg gccggccctc aaggttcgct gctgggcacg accgtgcaga
    14581 cggtcgacca cggggcgaat tgtacggccg tgaccgcggt gccgagcgcc ggccatcggt
    14641 ttgcgggttg gaccggcgac tacatcggca acgacaaccc gctgaccgtc acacatgtca
    14701 cggcgaacat gaccattacc gccaattttg gcagcaggat tcacaccgtt actttcgagg
    14761 cgggggagca cggctcgttg cagggcgata cccgccaaga ggttgattac ctcgccgact
    14821 gcactgcagt taccgccgtc gccgaccccg ggtattattt catcggctgg acgggcgatt
    14881 atgtcggcaa cgacaaccct ttggcgatca ccagtgtctc gacggacatg gtcatcagcg
    14941 ccaactttgc cgaccatcct cctccaagta acgtgcctcc cgctccgcgt ctcgtcgtgg
    15001 agattgcttc gaacgaacct ggcaacaacg ttgtgtgcaa gatggatgat gaactgtcgt
    15061 tcctgctcca cgtcgggaac atcggtgatc gggacgcgac ggatgtacga gtggtgatat
    15121 cactgccgga aaacgtcgaa ttcgtgaacg cgcgagtgcg agatgatatc gcggcgcgcg
    15181 gtaccccgga aaacgccagg ctggccgatc tccaggtcgt tctgaccatc gggtctctag
    15241 gtgcgggcga gacggtcgtg atcgacatgg tcttgcgggt
//
LOCUS       DAOTPW010000134        15110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3885_length_15110_cov_5.932248, whole genome shotgun sequence.
ACCESSION   DAOTPW010000134 DAOTPW010000000
VERSION     DAOTPW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15110
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_15110_cov_5.932248"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1429)
                     /locus_tag="P5316_12915"
     CDS             complement(89..1429)
                     /locus_tag="P5316_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR85903.1"
                     /translation="MPLGFVLEPAKQVPVAYDVDVAVAGAGVSGVFAALAAAREGAST
                     VLIDRFGSVGGNIGPGLYSPANEFVDRSRNIKQGAPDDPIKGEIAGLVKEFAERFSAL
                     KPHPQSVGDSNMAEYVSFCMLKEAGVKLILSAYVADPIIEGNRVRGLFVETKSGRQAV
                     RAKVVVDATGDADLARRAGLPVLYPQASGHEFDGHAPTGMGTRAIVAGIDPQKFNTPP
                     NAESRNLKVDIGDLAQLELVLQYNPAITGSLGFVKAELVRPHPKVNMGDARHISLLES
                     EVRRRIFEFILRCRDKIPGCENLYLLAVAPYFGSRGGPCIEGEYTLTMQDCIEGKRFD
                     DVIYVYGEAHALRHNCAQGECKWTDVPYRVMIPKRLDGLMAVGRSASGSPDTLLRIRE
                     ALMYMGQAAGTAAAMCAKSGVPPRRLDVKQLQKKLLDAGFYLGDHARLVELGLK"
     gene            1925..2329
                     /locus_tag="P5316_12920"
     CDS             1925..2329
                     /locus_tag="P5316_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HRR85904.1"
                     /translation="MDRWEIKVLYDGECPLCRREADLLRWLDGGRGKVLLEDITSPEF
                     DAGRYGVTTDQLMGQIHGVLSTGRLVKGMQVFRRAYDAVGWGWLLAPTNWPILRRCFD
                     AGYEWFARHRLRLTGRLGHCRTGRCGPPREAA"
     gene            2576..4339
                     /locus_tag="P5316_12925"
     CDS             2576..4339
                     /locus_tag="P5316_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR85905.1"
                     /translation="MSQDPESTAELLREIAVLKSRIDEMERQLAEHQEAWQAIRDNQE
                     SLEILLNAIPEAVLLIDPEGMILEANCEAARRFGLTREQILGRCAFDLIPPEVAEKRR
                     RQARSVIETGEPRRFLDRRDGRIIDNIMSPVFGADGRVARLAVLGIDVTNRQTAEEAL
                     RLAYDELETRVAQRTADLEEANRKLQEEINERKRAEMAAADRHALQEQLLQTQKMEAV
                     GQLAAGLAHDFNNLITVIRGYATQAAASLLPDHEARRALRSILDATQQAGGLTRSLQM
                     FSRKMPIERRPLNLRCLVEEAERFIRPTLPASITLEVLNDAASPLWVMADASQVHQVL
                     LNLAINARDAMPEGGRLRISVREIAHKHGRRQGDREALIAFSDTGIGIAEESLRRIFE
                     PFFTTKPRERNSGLGLAIVQSIVEEHGGRVQVESKLGQGTVFTIFLPLTEAAPLLDEG
                     EPVQPMPRGSGELILLAESNPHLAGIIALTLESLGYRVILASDGRLIAEIARKQRGQL
                     RLVILEADLPGCRGTACLRRMQEEGVLFPAVVLAGQSEAESMECPPHAVLLTRPFEMP
                     QLARTVYNMLAAAETRKESIE"
     gene            4336..4992
                     /locus_tag="P5316_12930"
     CDS             4336..4992
                     /locus_tag="P5316_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR85906.1"
                     /translation="MNKPITVLLVDDHAMVRGMLRGLLENTEDIRVVGSVANAADGVT
                     EAIRHKPDVMLMDIDLPGLSCFDAAQTIRIRSPETRVLFLSAFFHDRYIEQALNVQAW
                     GYITKSEPEESIIKAIRKVCSGVAYFSPEVQSRIVVDANGARLATPIRSRASTLSDRE
                     LEVLRYLARGMQKKEIAQVMCISVNTVNRHTTSLMNKLDIHDRVELARFAIREGLAEA
                     "
     assembly_gap    5119..5218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5363..6721)
                     /locus_tag="P5316_12935"
     CDS             complement(5363..6721)
                     /locus_tag="P5316_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85907.1"
                     /translation="MATMENCLLLCAWIGALSANLDLQPRVEVEEVVTAYTPANNGAG
                     PMWCYGSTTIARRGEEAFVSAIETGKDVPPLCNTRWQLWCRTSAGWKLEQSEKEYRQR
                     EPCPIGVFQNGPVFLSVNPSTQPPGTEYGPCRPLVLEFDPENPASPFREHEPAWAEGT
                     HFTDHSYRGFAADGARGELLLLNINAQTGAQFVSFRDRDGRWHARGKIEFPIRSCYPQ
                     VALRDRAAHVMAIGDIVEPKEEWRKYKFEKTGSQWDYVFRRLFYTYTPDINSKPFIAP
                     VEIDTVEETCGHITNLDLHIDEVGAAHVLYLRRPYQSELVRDKFFPGKPLTAHLEYMI
                     IRDGKIEAKTTPAETPAKGRAGLTPSYARFHVMPDGKLCIVVAGTMTGEAEAGTFGNY
                     VARIDRSEAKPTFVAVPLKHPFHSFFTNTPRGGSEPSELLDLFGTGSDSLNLRYARVR
                     VK"
     gene            complement(6802..9273)
                     /locus_tag="P5316_12940"
     CDS             complement(6802..9273)
                     /locus_tag="P5316_12940"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRR85908.1"
                     /translation="MILCLLDQLVLTMCVTAGISAEPWQAHDWREELRLPSPEFSLMP
                     FWFWNDDLDDDEIRRQMAAFREKGVHGFVIHARMGLPKDLEYMGPRWLGHVRFAVEEA
                     ARTGMRVCLYDEGMYPSGSAHGQVVQRNPSFAAQGLAVEAQRVDGPTTIEPAVATKGR
                     LVARVLIRPGEGESYRLETARAVPADAQTIEIPSGQWVVMTFLQVPTEGWIRGVHDGE
                     DDREPNAPPAADLLNPDAMRAFLQLAYEPYYETVGKHFGKTIIAMFTDEPSLTGRGGR
                     KDIKPWTGGFSEYFRAKAGYDLTTRLPALFFDVGEQTGKIRADYHSIIDQRLEETYYR
                     PLSQWCQRHGIALTGHPAASDEINCLRHFQIPGQDLVWRYVVPGDNSAIEGPHSTAAK
                     GTSSVARHDGRRRNSNEILGAYGWNLMMEEMKFVADHVLLRGQNLLYPHAFYYSVRGS
                     RLHERPPDVGPHNAWWPHYQLFADYTARLCGLLTDSEQVCDVAVAVCDNRLPWRAARW
                     LYQNQVDFNYVEESRFTEQAVVRDGHLEVGRMRYRTLIIDSDEDIPQAVSAAAASLER
                     TGGLVRRISTEPAEPLVSLKDRDVIVSPPAADLRYVHLVKREIDFYLLGNEGEAHLDT
                     HLTVRSVGEAEWFDAWKGVFSPATVVVATDSTITVPLHLPRRETRVLCIDTSKPPTVA
                     SVREPGGFADLISLEGSWEIAVGGKEMRQEELGDWTAWSGMEDEVGPVHYQRSFQVHR
                     DQGKRYQLDLGQVGDWVVVRLNGQELGVRFWSPFTWGITDALRDGENTLVVEVTGSLA
                     NRHDPKKRRPAGLMGPVRVLATSRY"
     gene            9540..11327
                     /locus_tag="P5316_12945"
     CDS             9540..11327
                     /locus_tag="P5316_12945"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRR85909.1"
                     /translation="MRSFPLLLTLGLLVIAVVLVAADRVSAVVIAEDGHGEAVVVVSE
                     NAAPGEKHAAQELASFLKQVTGADFEVLASADGDKPRLLVGPEAARLADPKFTADGLG
                     AEGLIIRTVGKDLILAGGRPRGTLYAVYTFLEDHIGCRWWAPGASSIPRKPTLAFHRL
                     GVKYVPPLEYREPFWFSGFDGDWAARNKVNGATPRLEEKHGGKHVIEGFVHTFNALIP
                     PTKYFKDHPEWFSEIQGRRVQEHSQLCLTNEQMRAELVKNLKDRLRSNPAATMASVSQ
                     NDWANNCQCPRCAAVDKEEGSPAGSMIRFVNAVAEEIEKEFPNVAISTLAYQYTRKPP
                     LKVRPRPNVVVWLCSIECSFSKPLTDEINADFRRDIEGWSKICKRLYVWDYTTNFSHY
                     VLPHPNLCVLGPNVKFFADHNVVGLFEQGAYHTNGAEMMELRAWVLAKLLWNPSLNAD
                     LLTGEFLAGYYGSAARHIYAYLEIMHKAVGTCGDRLGCFSPPDAKFLNLETLSEGWKH
                     LKAAEEAARNDETLLLRVRTAELPVLYTFIIQWDRLRKEAADAKHSWPVADTQQAVYE
                     QFMKTAEAIKMTHVAEGRGIDWLKSQVRP"
     gene            11659..13134
                     /locus_tag="P5316_12950"
     CDS             11659..13134
                     /locus_tag="P5316_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRR85910.1"
                     /translation="MTRSRSLRSEVVRCLVFAGAIGLALAVPSAFGADRPTTPPAGAM
                     KVAEDLSAAFEYVAESVKASVVNITSVKTIKPTMRGRQELPEEIPFPEFFERFLRPDV
                     PKGGFKQQGFGAGVIVSEDGHILTNNHVVADADEITVTLTDKREYKAKIIGTDPKTDL
                     AVVKIKADNLMPAKLGDSDQVRVGEWVVAVGHPFGLSYTITTGIVSAKGRANRAVAEY
                     EDFIQTDAAINPGNSGGPLVNLKGEVIGINTAIFSRSGGYMGIGFAIPSNMAKSIMQS
                     LIDHGKVVRGWLGVVIQNLDEAMAESFGFKGTNGVLVGDVTPDSPAEKAGMKQGDIIL
                     KFGGKETNDTNQLRQMVAVTSPGTKVKVEVFREGERETLEVTVGELESGLATIKGHEA
                     EDEDLGMTLKNLTPEIAEQLGLKQTKGVVVTAVEPFSVAAKAGLRPGDVIVAVQNKPV
                     DTLAEFNKAMSKQDLKKGVRLTVLSEGMRRFVLLKSGGSDE"
     gene            13504..14481
                     /locus_tag="P5316_12955"
     CDS             13504..14481
                     /locus_tag="P5316_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRR85911.1"
                     /translation="MASGYCVGVDLGGTNIAAGLLDSEGNVKSSLSIPTEVDKGRDQV
                     VANIIAAAEKAISQAGVDRKDVLGIGVGSPGPMSHRRGMVINPGNLPCMQNVPLREIM
                     KEKTGIPTTIENDANAAAWGEFWAGAGKDVNDLVMYTLGTGVGGGVIVEGKLLRGYFE
                     NGAELGHVIVNPGGRLCSCGQRGCVEAYSSAYFLARHAEELIKQGRPSVLKTRMDAGE
                     FLRAEHIVEAAKAGDALAQEVWDKACYYLAVAIVGMRHVTNPQRVVLAGGLIAAGDFL
                     LAPIRKYAAEMAWKLLDDGPEICFATLGNNAGFVGAAGCAWEARRTGDW"
BASE COUNT         2906 a         4526 c         4728 g         2850 t
ORIGIN      
        1 ggtccaacaa cccgaaaaaa atctagaatc gccctttggt gttcaacttg ggctaatcga
       61 ccaacagacc aacaggccaa caaccgcctc atttgagccc cagctcaacc aatcgcgcgt
      121 ggtcgcccag gtagaagcct gcgtcgagca gcttcttctg gagctgcttg acgtccagcc
      181 tgcgaggcgg gacgccgctc ttggcgcaca tggcggccgc agtcccggcc gcctgcccca
      241 tgtacatcag ggcttcccgt atgcgcagca gcgtgtcggg cgagcccgag gcgctgcggc
      301 cgacggccat caggccatcc agtctcttcg gaatcatgac acgatagggc acatcggtcc
      361 atttgcattc accctgcgca cagttgtggc gaagcgcgtg tgcctctccg tagacgtaga
      421 tgacgtcatc gaatcgcttc ccctcgatgc aatcctgcat ggtgagagtg tactcgcctt
      481 cgatgcatgg tccgccgcgg gagccaaagt aaggcgcgac ggcaagcagg tagaggtttt
      541 cgcagccggg aatcttgtcc cggcaccgca gtatgaactc gaagatgcgc cgccgtacct
      601 ctgattccag aagcgagata tgccgggcgt ctcccatgtt gaccttggga tgcggcctga
      661 cgagctcggc tttcacaaat cccaggctgc cggtgatggc gggattgtac tgaagcacga
      721 gctccaactg ggccagatcc ccgatatcga ccttcagatt gcgcgattcc gcgttgggtg
      781 gcgtgttgaa cttctgggga tcgatgcccg cgacgatggc gcgcgtgccc atgcccgtgg
      841 gggcgtgccc gtcgaactca tggccgcttg cctgcgggta gagcaccggt agacccgctc
      901 ggcgggccag atccgcgtcg ccggttgcgt cgacgacgac cttcgctcgc acggcctgac
      961 ggccggactt ggtctccacg aacaggccgc ggactctgtt gccctcgatg atggggtcgg
     1021 ccacataggc cgacaggatc agcttgacgc ccgcctcctt gagcatgcag aaggagacgt
     1081 actcagccat attcgagtcg ccgacggatt gtggatgcgg cttcagggcg gagaaccgct
     1141 cggcaaattc cttaaccaaa ccggcgatct cgcccttgat cggatcgtcg ggcgcgccct
     1201 gcttgatgtt gcggctgcgg tcgacgaatt cgttggccgg cgagtacaga cccggcccga
     1261 tgtttcctcc gacgctgcca aagcggtcga tcaggacggt gctggccccc tcccgagcgg
     1321 cggccaaggc cgcaaagacg cccgaaacac ctgccccggc gactgccacg tcgacgtcat
     1381 aggctacggg aacctgcttg gcgggctcca atacgaaccc gagcggcacc gcagccctgc
     1441 ccggaccgtg ctcgtctgtg gcttgggtcg cagggacatt cggcagaagt gaagaaaccg
     1501 acgcaatggc taacagggcc ccgaacatag ttgtgctcca atacgaacgg agaaccggac
     1561 cgcaggccac gaccgaaccg caagccgcat gcgcctgcac tcattttgcc atcctgtcat
     1621 cgatgttccc gatcgtcaac aaacggtcca gggcgccttc ttgagcgagg aagaaaggcc
     1681 gctgttttcg tcggtcgttt ctgatcgcgg gttcggcacc gccgtcgtga gggactggac
     1741 gctgctccac cttctgtagc tcatcccgcg atgcctcagg gcacgttcag aaccagccgc
     1801 tggccagatg gccgttagtc cattgccccg caactgttta tgatcgcagc tcgacgctgt
     1861 ggcaatcgtt gatgggtcga cctacgatgg atcagacaag taccagataa aggggagtgc
     1921 agacatggac cgatgggaaa tcaaagtcct gtacgatggc gagtgcccgc tgtgtcgccg
     1981 agaagccgac ctgctccgct ggcttgacgg cgggcgcggc aaggtgttgc tggaggacat
     2041 caccagcccg gagtttgacg ccggccgtta cggagtaacg acggatcaac tgatgggcca
     2101 gattcatggc gtgctgagca ccgggcggct ggtcaagggc atgcaggttt tcagacgagc
     2161 ctacgacgcc gtggggtggg gctggctgct ggctccgacg aactggccga tccttcgccg
     2221 ctgcttcgac gccggatacg agtggtttgc caggcatcgg ctgcggctga cggggcgatt
     2281 gggccattgt cgcacgggtc ggtgcggacc gccgcgcgag gccgcgtgac agctcgcgca
     2341 gcaacatggc ctttccgtca tctgggatct tggacacggc atcaatcgcg ggtatattcc
     2401 atctgagctg aggggcagag gcttgcgcgt cccggcatca gagggggcat tcgatgtttc
     2461 ggggcttgtt gctgcacctt ggcgcgaccg ttcgtagcca tctgtatgaa gggcgtttct
     2521 tgctcgccgg acagcaagtg ccggactcgc tgccgcacag tgtcttgaga gatcgatgag
     2581 tcaggatccg gaatcgaccg cagagcttct tcgcgagatc gccgttctca agagccgcat
     2641 cgacgagatg gagcggcagc ttgcggagca ccaggaggcc tggcaggcga ttcgggacaa
     2701 ccaggagtcg ctggaaatcc tgctcaacgc aattcctgag gcggtccttc tgattgatcc
     2761 cgagggtatg atcctcgaag ccaattgcga ggcggcccgg agattcggcc tgacacggga
     2821 acagattctc ggtcggtgtg cgtttgacct gattccgccg gaggtggccg agaagcgtcg
     2881 ccgtcaggct cgcagcgtca tcgagacggg agaaccgagg cgatttctcg accgacggga
     2941 cggccggatc atcgacaaca tcatgtctcc ggtgttcggt gcggacggcc gtgtcgcccg
     3001 gctggccgtg ttgggaatcg acgtgaccaa tcggcagacc gccgaggaag ccctccggct
     3061 ggcttatgac gaactcgaga cgcgtgtcgc tcaacgaacg gcggatcttg aggaggcgaa
     3121 tcgcaagctc caggaggaga tcaatgaacg gaaacgggcc gagatggcgg ccgccgatcg
     3181 gcacgccctt caggaacagc tcctgcagac tcagaagatg gaagccgtcg gtcagcttgc
     3241 cgcgggcttg gctcacgatt tcaacaacct catcaccgtc attcgcgggt atgccacgca
     3301 ggccgcggcc tcactgttgc cggaccatga ggcccgccgg gccctgcgat ccatccttga
     3361 tgccacgcag caggccggcg gactgacccg ttcgctgcag atgttcagcc gcaagatgcc
     3421 gatcgagcga cggccgttga atctgcgatg tctcgtcgag gaggctgagc gcttcatccg
     3481 accgacgttg ccggcgtcga tcacgctgga agtcctgaat gacgccgctt cgccgctatg
     3541 ggtgatggcc gacgccagcc aggtgcacca agtgcttctg aatctggcga tcaacgcccg
     3601 tgacgcgatg cccgagggcg gccgactgcg gatttcggtg cgcgagattg cacacaagca
     3661 cgggcggcga cagggagacc gagaggcgct gattgcgttc agcgacacgg gcatcgggat
     3721 cgcagaggag agtctgcgca gaatcttcga gccgtttttc accaccaagc ctcgcgagcg
     3781 gaactcgggg ctgggcctgg cgatcgtcca gagcatcgtc gaggaacacg gcggccgggt
     3841 gcaggtcgaa tcaaagcttg gacaggggac ggtgttcacg atctttctac cgctcacgga
     3901 ggcggcgccg cttcttgatg agggagagcc cgttcagccc atgccaagag gtagtggcga
     3961 gctgatcctc ttggccgaga gcaatcctca cctggcgggc atcattgcct tgacgctcga
     4021 atcgctgggg tatcgcgtga ttctggcgag cgatggtcgg cttatcgccg agatcgcgcg
     4081 gaaacaacgg gggcaattgc ggcttgtaat ccttgaggcc gatctgccgg ggtgcagagg
     4141 cacggcctgt ctgagacgga tgcaggaaga gggggtgttg tttccggccg ttgtcctggc
     4201 aggccagtcc gaggccgagt ccatggaatg ccctccccat gcggttctgc tgacccggcc
     4261 cttcgagatg ccgcagttgg cccgaacggt gtacaatatg cttgcggccg ctgagaccag
     4321 gaaggagagc atcgagtgaa caaaccgatc acggttctgc tggtcgacga tcatgcgatg
     4381 gtccggggga tgctccgcgg gctgttggaa aacacggagg acattcgagt ggtcggcagc
     4441 gtcgccaacg ccgccgacgg cgttacggag gcgatccgcc acaagcccga tgtgatgctc
     4501 atggacatcg acctgcccgg cctgagctgc tttgacgccg cacagacgat tcgcatccgc
     4561 tcgccggaga cgcgggtgtt gtttctcagc gcgttcttcc acgatcgtta cattgagcag
     4621 gccctcaacg tgcaggcgtg gggttacatc accaagagcg agccggagga gtcgatcatc
     4681 aaggccatcc gcaaggtgtg ctcgggcgtg gcgtatttct cgccggaggt gcagagccgg
     4741 atcgtcgtcg acgccaacgg cgcccgcctg gctacgccga tccgctcgcg ggcctcgacg
     4801 ctgtccgacc gtgaactcga agtccttcgc tatcttgccc gcggcatgca gaagaaggaa
     4861 atcgcccagg tcatgtgcat cagtgtcaat acggtcaacc ggcacaccac cagcctcatg
     4921 aacaagctcg acatccatga ccgggtggaa ctggcacgct ttgccatacg ggaggggctg
     4981 gcggaggcgt gacgtcagag tcttcagttc tcagttttca gtcttcagtc ctacaagccc
     5041 gctgatgcca gaatcccttc tgacaccatt ccttcgacga atcccttcgt catgctgcca
     5101 aggcggcttc aggctatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     5221 gccagaatcc tttctggcac catcccttcg acgaatctct tcgtcatgct gcccagtgcc
     5281 gcccatgttt tcccccggcc ccgagcccct ccgcgaatgg ccgacggggg cgtcggccct
     5341 actgtcggag tcgaagacca tgctacttca cgcgaactcg tgcatatcgc aggttgagcg
     5401 aatcgctgcc cgtgccgaac aaatcgagta gctcggaggg ctctgaaccg ccgcgaggag
     5461 tgttggtgaa gaaactgtgg aacgggtgct tgaggggcac ggcaacgaaa gtcggcttgg
     5521 cttccgaccg gtcgatccgg gcgacgtaat tgccgaatgt gcccgcctcc gcttcgccgg
     5581 tcatcgtgcc ggcgaccaca atgcacagtt ttccgtccgg cattacgtga aaacgggcgt
     5641 acgacggcgt cagaccggcc ctgcccttgg ccggtgtctc ggccggcgtg gtcttcgcct
     5701 cgatcttgcc gtccctgatg atcatgtact caagatgcgc ggtcagtggt ttgccgggaa
     5761 agaacttgtc gcggaccaat tcgctctggt acggacgcct caggtagagc acatgggccg
     5821 ctccaacctc atcgatatgc aggtccaggt tggtgatgtg gccgcaggtc tcctccacgg
     5881 tgtcgatttc gaccggcgca atgaacggct tgctgttaat gtccggggtg tacgtgtaga
     5941 atagccgccg gaacacgtag tcccactggc tgccggtctt ctcgaatttg tacttgcgcc
     6001 attcctcttt tggctcgacg atgtcgccga tggccatgac gtgcgccgcc cggtctcgta
     6061 gggccacctg agggtagcac gaccggattg ggaattcgat tttgcctcgg gcatgccatc
     6121 ggccgtcgcg gtcgcggaac gaaacgaatt gggccccggt ctgggcattg atgttaagca
     6181 gcagcagttc gcccctagcc ccgtcggccg cgaacccgcg atacgagtgg tcggtgaagt
     6241 gagtgccctc ggcccaggct ggttcgtgtt cgcgaaacgg ggaggccggg ttctcgggat
     6301 cgaattcgag caccaacggc cggcaaggac catactcggt gcccggcggc tgggtcgaag
     6361 ggttcacgga caggaacacc gggccgttct ggaagacgcc gatcggacac ggctcgcgct
     6421 gacgatactc tttctcgctc tgttccagtt tccatccggc cgacgtgcgg caccacaact
     6481 gccatcgcgt gttgcacaac ggcggcacat ccttgcccgt ctcgatcgcc gagacaaacg
     6541 cctcttcgcc tcgccgggca atcgtcgtcg acccgtagca ccacatcggc ccggcgccgt
     6601 tgttggccgg ggtgtacgcg gtgactacct cctcgacctc gacacgaggt tgaagatcga
     6661 ggttcgcaga gagcgcacca atccatgcgc acaacaacag acagttttcc atagttgcca
     6721 cacctcatca tcctgtcgcc gacaacagcc agtgaactgt cagccacttg acacgccagc
     6781 ggttcactgg agcgattcac gtcagtaccg cgaggttgcg aggactcgca ccggccccat
     6841 cagccctgcc gggcggcgtt tcttcgggtc gtgacgattg gcgagcgatc cggtgacttc
     6901 aaccacgaga gtgttctcgc cgtcgcgcag ggcatccgta atgccccacg tgaaggggga
     6961 ccagaaacgc acgcccagct cctgcccgtt caatcggaca accacccagt ctccgacctg
     7021 gccaagatcg agctgatagc gcttgccctg atctcggtgc acctggaatg atctttggta
     7081 atgcaccggc ccgacttcgt cctccatgcc ggaccacgcg gtccagtcac ccaattcctc
     7141 ctgccgcatt tcctttccgc ccacggcgat ttcccacgat ccctccagac taatgagatc
     7201 cgcaaacccg ccaggttcgc gcacagaggc gactgtcggc ggctttgacg tgtcaataca
     7261 cagcacgcgc gtttcccgtc ggggcaggtg cagtggaacc gtgatggtcg aatccgtcgc
     7321 cacgacgaca gtcgccggac tgaagacccc tttccacgca tcgaaccatt cggcttcacc
     7381 gacgctgcga acggtcaggt gcgtgtccag atgtgcctcg ccttcgttgc ccaataggta
     7441 gaaatcgatt tctcgtttga ccaggtggac gtagcgaagg tcagcggcag gaggactgac
     7501 aatcacgtcg cggtctttca gggaaaccag cggttcggcc ggctcggtgc tgattcggcg
     7561 gaccaatccg cccgtccgtt ccaaagaagc ggccgctgcg ctcacggcct ggggaatgtc
     7621 ttcgtcgctg tcgatgatca aggtgcgata gcgcatcctg ccgacctcaa gatggccatc
     7681 acgaaccacg gcctgttccg tgaatcgaga ctcctcgaca tagttgaaat ccacctggtt
     7741 ctgatagagc catcgggcgg cccgccacgg caagcggttg tcgcagaccg ccaccgctac
     7801 gtcgcagacc tgctcggaat cagttaacaa cccacataga cgagcggtgt aatccgcaaa
     7861 cagttggtag tggggccacc aggcattgtg gggtcccaca tccggcggtc gctcgtgaag
     7921 ccgactcccc cgaacggaat agtagaaagc gtgcggatag agcaggttct ggcctcgcag
     7981 gagcacatgg tcggccacga acttcatttc ctccatcatg aggttccatc cgtaagctcc
     8041 gagtatctcg ttactgttgc gccgcctgcc atcatggcgg gcaacactgc tggtaccctt
     8101 ggcggccgtg ctgtgcgggc cctcgatggc ggagttgtct ccgggcacga cgtaacgcca
     8161 tacgagatcc tgtccgggga tctggaaatg gcgaaggcag ttgatctcgt cgctggctgc
     8221 cgggtggcct gtcagtgcga taccgtgccg ctggcaccac tgtgagagcg gccggtaata
     8281 ggtttcctcc aatcgctgat cgattatgct gtggtaatct gcgcgaatct tgccggtctg
     8341 ctctccgacg tcaaagaaca aggcgggcag tcgtgtcgtc aggtcatagc cggccttcgc
     8401 gcggaagtac tccgagaatc cccccgtcca cggtttgatg tctttacggc caccccgccc
     8461 tgtcaggctt ggctcatcgg tgaacatcgc gatgatcgtc ttgccaaagt gcttgccgac
     8521 ggtttcgtag tagggttcgt aggccagttg gaggaaagcc cgcatcgcgt cgggattcag
     8581 caggtccgct gcgggcgggg cattcggttc tctgtcgtct tccccgtcgt gcacgccacg
     8641 aatccaccct tcggtgggca cctggaggaa ggtcatcacg acccactggc ctgacgggat
     8701 ttcgatcgtc tgtgcgtcag caggaacggc acgagctgtc tcgagccggt acgattcccc
     8761 ctcgccaggc cgaatcagaa ctcgggcaac cagtcgtcct ttggtggcaa cggcaggctc
     8821 gatcgtcgtc ggcccatcca cgcgctgggc ctcgactgcc agaccttggg cagcgaacga
     8881 tggattgcgt tggacgacct gtccgtgggc ggagcccgac gggtacatgc cctcgtcata
     8941 cagacacacc cgcatgcctg ttcgcgcggc ctcctccact gcaaaccgca catgccccag
     9001 ccagcggggg cccatgtact caaggtcctt cggtagtccc attctcgcat ggatgacaaa
     9061 gccatgaacg cccttctccc gaaaggcagc catctgccgg cgaatctcgt cgtcgtccag
     9121 atcgtcattc cagaaccaga acggcatcag cgagaattcg ggcgacggaa ggcgcagctc
     9181 ctcccgccag tcgtgtgcct gccaaggctc ggcggaaatg ccggccgtta cgcacatagt
     9241 gaggacaagc tgatcgagaa ggcacagaat catgatgctc gctccgacga agccggccaa
     9301 tggctcacag attggcaacc gtcgttgctg gtacagtcgt atccagctcg aacacgcagt
     9361 ataggacgac ggtgagttgg cttccaggat gccggccagg agctcctttg ccggcatctg
     9421 caccctatgc cggcgacggt ccggccaaag atcgcttctg gccaacacgc caggcttgtg
     9481 ttaacgtctt cggtgacatc gtgcaggcaa gctttcagct tcgcataagg agaagacaga
     9541 tgcgctcgtt tccgttgctg ctgacgctcg gattgctggt aattgctgtc gttcttgtgg
     9601 cggcagaccg cgtttcggct gtcgtgattg ccgaagatgg tcatggcgag gcggttgtcg
     9661 tggtctcgga gaacgctgcg ccgggtgaga aacacgcagc gcaggagctg gcctcgttcc
     9721 tcaaacaagt cacgggtgcc gatttcgagg ttctggcgtc agccgacggc gacaagcccc
     9781 gacttctggt cggtcccgag gcggcgaggc tggcggaccc caagtttacc gccgatgggc
     9841 ttggcgccga gggcctgatc atacgcaccg tcggtaagga cctgattctt gcaggcggcc
     9901 ggccccgcgg cacgctgtat gcggtctata ccttcctgga agaccatatc ggctgccgat
     9961 ggtgggctcc gggcgcaagc agcatcccga gaaaaccgac gctggccttc cataggctcg
    10021 gagtcaagta cgtcccgccg ctggagtacc gggagccgtt ctggttcagc ggttttgacg
    10081 gcgactgggc cgctcgcaac aaggtcaacg gagccacacc gcgattggag gagaagcacg
    10141 gcggcaaaca tgtgatcgag ggtttcgtcc acaccttcaa cgccctgatc ccgccgacca
    10201 agtacttcaa ggaccatccg gagtggttca gcgagatcca gggcaggcgc gtgcaggagc
    10261 atagccagtt gtgcctgacc aacgagcaaa tgcgggctga actggtcaag aacctgaagg
    10321 atcgcctgcg cagcaacccc gccgcgacta tggcgtcggt gtcgcagaac gattgggcga
    10381 acaactgcca gtgcccgagg tgtgcggccg tcgacaagga agagggcagt ccggccgggt
    10441 cgatgatccg cttcgtcaac gccgtggccg aggagatcga gaaggagttc cccaatgtcg
    10501 cgatcagcac gctggcttac cagtacaccc gcaaaccgcc gctcaaggtt cggccgaggc
    10561 caaacgtcgt ggtctggctg tgcagcatcg agtgctcgtt cagcaagccg ctgaccgacg
    10621 agatcaacgc cgatttccgc cgtgatatcg agggctggtc gaagatctgc aagcgtttgt
    10681 acgtatggga ttatactacc aacttctccc actacgtgct accgcacccg aacctgtgcg
    10741 ttctcggccc gaacgtcaag ttctttgccg accacaacgt cgtcgggctg tttgaacagg
    10801 gggcctatca caccaacggc gccgagatga tggaactgcg ggcatgggtg ctcgccaagc
    10861 tgctgtggaa cccgtctctg aacgccgact tactgacggg cgagttcctc gcgggttatt
    10921 atggctctgc tgcacgccat atctacgcat atcttgagat catgcacaag gctgttggca
    10981 cgtgtggtga ccgccttggc tgtttctccc cgccggatgc caagttcctg aaccttgaga
    11041 cgctgagcga ggggtggaag catctaaagg cagcagaaga agccgcacgc aacgatgaga
    11101 ctttgcttct ccgtgttcgc acggccgaac tgccggtcct gtacacgttc atcattcaat
    11161 gggaccggct gcggaaggaa gccgctgatg cgaagcactc ttggccggtt gccgacactc
    11221 aacaggctgt ctatgagcag ttcatgaaaa ccgcggaggc gatcaagatg acccacgtgg
    11281 ccgagggccg aggcatcgac tggctgaagt ctcaggtacg cccctgaccg tcacgggcgg
    11341 atcgcatgcc cggatctctc tgcttggcgc cgccggtgcg gggccggttg ctcgcaccct
    11401 agtccggctc ggtcttgatc tccagcggtg ttcggggctc gcggacctcg ttcgcggagg
    11461 ccggcccgcg ccctgagacg gaacattgtt gcacgagccg ttgtactata acgccttatc
    11521 agtgcgacac ttacaatctc atatctgtca ggtgtccggc cgctgctcgt ttcatgaaca
    11581 gctacggtat tccccgcgta tagaagaaca agtggagcgt taccggaaga atcgcaagcg
    11641 tcagggagaa agacaacaat gacgagaagc aggtcgttga gaagcgaggt tgtacggtgt
    11701 ctggtgtttg cgggcgcaat tgggcttgcc ctcgcggtgc cgtcggcttt tggagcagac
    11761 cgacctacga cgccgccggc cggcgcgatg aaggtggctg aagacctgtc cgcggcgttc
    11821 gagtatgtgg ccgagagtgt taaggcctcc gtggtcaaca tcacttcggt gaagacgatc
    11881 aaaccgacga tgcgagggag gcaggagttg ccggaggaga taccatttcc ggaattcttt
    11941 gagcgtttcc tgcgacccga tgttccaaaa gggggtttca agcagcaggg attcggcgcg
    12001 ggggtgatcg tctccgaaga cggccatatc ttgaccaaca atcacgtcgt ggccgacgcg
    12061 gatgagatca ccgtgaccct taccgacaag cgggagtaca aggccaagat catcggcacc
    12121 gacccgaaga ccgatctggc cgtcgtgaag atcaaggccg acaacctcat gcccgcgaaa
    12181 ctcggcgatt cggatcaagt gcgggtgggc gagtgggtgg tagccgtcgg gcaccccttc
    12241 ggtctgtcgt ataccattac cacggggatc gtcagtgcta aagggcgcgc gaaccgggcc
    12301 gttgccgagt acgaggattt catccagacg gacgcggcta tcaaccccgg caacagcggc
    12361 ggaccactgg tgaatctcaa gggcgaggtc atcggcatca acaccgccat cttcagccga
    12421 agcggggggt acatgggcat cgggtttgcg atcccgagca acatggccaa atccatcatg
    12481 cagagcctta tcgaccacgg caaggtcgtg cgcggctggt tgggggttgt gattcagaat
    12541 ctcgacgaag ccatggccga gtccttcgga ttcaagggta ccaacggggt actggttggc
    12601 gacgtgacgc ccgacagccc ggcggagaaa gccgggatga agcagggcga catcatcctg
    12661 aagttcggtg gcaaggaaac gaacgacacc aatcagctcc gtcagatggt ggccgtcacc
    12721 tcgccgggaa ccaaggttaa ggttgaggtt tttcgtgaag gggagcgcga gactctggaa
    12781 gtaaccgtag gtgagctcga aagcggcctg gccaccatca aaggccatga agccgaagat
    12841 gaagaccttg gcatgaccct caagaacctc acgccggaaa tcgccgagca actggggctc
    12901 aagcaaacca agggagtggt cgtcacggcg gtcgagccgt tcagcgttgc ggccaaggcc
    12961 ggtctgcgtc ccggagacgt cattgtcgcc gttcagaaca aaccggtcga cacgttggcc
    13021 gagttcaaca aggcgatgag caagcaggac ctcaagaagg gggtgcgcct cacggttctg
    13081 agtgagggca tgagacggtt tgtcctgctc aagagcgggg gcagcgacga gtagcaaccc
    13141 tcgggttgtc cttgacaatc tcgtcggacc cgctgcggac accgtcgtcg tgcgagctcg
    13201 tttccgggcc gtgattgcac gcaagcggtg agtgcgccgc agcgaaaccc tgcccgtggt
    13261 tcgtcacagg cggtcgggat tgcagcaccg gtcatccagg ccatgagtcc tgttcctgcc
    13321 cggcttgggt gggaacccgc cggaagcgct acacaccgtc gtcattttcc gccagattcg
    13381 ccccattgag tccgacgcgg atctgttcta ccatcaccgc gcacggcggg gcctgatgac
    13441 cggtcatccg ctgccggcgc ggcggagtca gcgggcggca cacgacacag aggtgaatca
    13501 accatggcga gcggctattg cgtgggcgtg gatctcggcg gcacgaacat cgcggcaggt
    13561 ctgctggaca gcgagggcaa tgtcaagtcg agcctcagca taccgaccga ggtcgataaa
    13621 gggcgagacc aggtagtcgc caacatcatc gccgccgccg agaaggccat ttcacaggcc
    13681 ggcgtcgacc gaaaggacgt gctggggatc ggtgtcggct cgcccgggcc gatgagccat
    13741 cgtcgaggca tggtgatcaa tccgggtaac ctgccctgca tgcagaacgt tccgctgcga
    13801 gagatcatga aagaaaagac gggcattccg acgacgatcg agaacgacgc caacgcggcg
    13861 gcatggggtg agttctgggc cggtgccggc aaggacgtca acgacctggt catgtacacg
    13921 ctcggaaccg gtgtgggcgg cggggtcatc gtcgagggca agctgctccg cggttatttc
    13981 gaaaacgggg ccgaactggg ccatgtcatc gtcaatcccg gcggtcgatt gtgttcctgc
    14041 ggccagcgag gttgcgtcga ggcctactcg tccgcttatt tccttgcccg ccatgccgag
    14101 gagctgatca agcagggaag accgagtgtg ttgaagaccc gcatggatgc gggagagttt
    14161 ctgcgagccg agcacatcgt cgaagcggcc aaggcgggtg acgcgctcgc gcaggaggtc
    14221 tgggacaagg cctgctacta cctggccgtg gccattgtcg gcatgcgtca cgtgaccaac
    14281 cctcaacgcg tggttctggc cggcggcctg atcgcggccg gcgacttcct gcttgctccc
    14341 atccgcaagt atgcggcaga gatggcctgg aaactcctcg atgacggtcc tgaaatctgc
    14401 tttgccacgc tcggcaacaa cgccggcttt gtcggagccg ccggatgcgc ttgggaagcc
    14461 cggcgaaccg gagactggtg agcagagttc accgtcaggg gcatattcac cgcaatacct
    14521 gatccggcat tcccttggtc cgggagtcga gtatcgcggg ttgcttcgcc gttgcgcagg
    14581 gttctgggcc catggcgagg cgtctggacc gaataactgc ggcaagtcaa gtcagtcgtc
    14641 cggttctccg ataggctcgt cagtggcaag gcagtaagtc gcggagcacg ccgatcacct
    14701 ggaccggact ccttctgagc actaccagcc actcatgaag ggcaaataac cgccggcgaa
    14761 ttccgtctcg gcatgaatgc cgcctgagcc gccggcgaac aagtcgcaag ggcctggcgg
    14821 accgcaaggg gggcggcccg tcaggtccgg tttcttgcgc ctaatgggtg taaaccgccg
    14881 cgagcatcgc cgcagaacga gcgcgttata gaaccttacg gccagacatc tgcgcggctc
    14941 gcgcgcatgt ttctggatcg cagagcgctt gggttgcggc ccgcgaaaag gcggtccgcc
    15001 ttgggtaccg ggttgccatg cgtaataacc tgcaaaaagg gatctcgccg gatcccatct
    15061 aacccaagtt gaacaccaaa gggcgattct agattttttt cgggttgttg
//
LOCUS       DAOTPW010000135        14997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3915_length_14997_cov_6.347945, whole genome shotgun sequence.
ACCESSION   DAOTPW010000135 DAOTPW010000000
VERSION     DAOTPW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14997
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_14997_cov_6.347945"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..732)
                     /locus_tag="P5316_12960"
     CDS             complement(37..732)
                     /locus_tag="P5316_12960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR85912.1"
                     /translation="MSERFVELTGIAVVVFDLDDTLYPERQFVWSGFEAVANWLRQQM
                     DCPDDPARRMRELFESGCRRRVFDELLAEWRCPHAEDWVRQMIACYRDHNPVIRLYPD
                     AEAVLLKWQGLFQLSLISDGPVRMQQNKVRALGLESLLRPLVLTDRWGEAFRKPHQRA
                     FEYIESVTGCRGPECLYIGDNPQKDFVAPNQMAWRSVCVRHQDGIYANTEAPPEGQPE
                     FLVSELSYILISK"
     gene            complement(875..1891)
                     /locus_tag="P5316_12965"
     CDS             complement(875..1891)
                     /locus_tag="P5316_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRR85913.1"
                     /translation="MTGIQAMYGAGARRRDVGILFTCAGRRIELISAFIRAARALGLR
                     PSIHVADTESHFAAACIADEAHQVPPTHSTDYIRSLLRIVKRRRIDILVPLTDTDLVK
                     LAESRDEFARSSCIALISSPAVVHTCRDKLLTYRFLTSHRIDTPATWTAQEALARRRH
                     RFPYFLKPRRGSASRGSFVIHHRRDLEAMVPRVPEAIVQEFVPGLEHTLDVYAGFDGC
                     PRCVVPRERIEVRGGEVTKARTVKNRRIMATGVRVVEALAGCVGLITIQLILSPDGRI
                     RVIEINPRFGGGVPLAIHAGADFPKWLLTEWLGRKPRIRLDQFRDGEIMLRFHQSFFV
                     RDRD"
     gene            2558..3829
                     /locus_tag="P5316_12970"
     CDS             2558..3829
                     /locus_tag="P5316_12970"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HRR85914.1"
                     /translation="MATSPTEAELLRCAVPTPSSGAERDATPEVDALGMLGSQPGTAS
                     ILVADDDPDVRALIRHLLKKAGYEVLEAEDGEQVLAMLEKRLPELILLDCQMPRLDGF
                     STCLELKKNPNYADLPVVFLTVRSDPSDKARGFAVGGEDYITKPIERQEFLARIRSRL
                     ELSLNRRLLRRKATIYEAVSMESADRLEDVRDGQVSLLTPPSSLPELKLGVRFQPAHE
                     AGGDFYEIARLCDDEFGFLVSDVSGHDLSVPFVTGALKALAATFLNESLTPLEVLSLH
                     NSSLLKFLSEGRYVTACCARFSKLTMEVEVANAAHPPAFFQSSRHEGEFIDLSGDILG
                     MLETARFESKRFPVERGERLFLYTDGLIESRRGPDGRLSVVQGMAWLKEGLLARRALP
                     IDQVVDSVVDELLCDCGGKAEDDVVLMGIEF"
     gene            3838..4275
                     /locus_tag="P5316_12975"
     CDS             3838..4275
                     /locus_tag="P5316_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR85915.1"
                     /translation="MFDIEHTPDALRITMSPSFENIDRADDLCAQELDRRQVPVNMFA
                     VRILLREAALNAVTHGCREAPEGQVRVALVFATDEIKLIVEDNGPGFVWENREAGFDV
                     TGDGGRGLPLMKTYASQVTFNEAGNRVELSVRHELTPPVGCVI"
     gene            4293..4601
                     /locus_tag="P5316_12980"
     CDS             4293..4601
                     /locus_tag="P5316_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRR85916.1"
                     /translation="MTSVTETRQTHTVRPDYDIVASHTEQFRAQLQDLISQGHVHLVI
                     DLGDVKMIDSKGLAVLMLCHKSVSAAGGSLTVLTRNQDFRHLFHVMRMDEHFTIAESL
                     "
     gene            4642..7764
                     /locus_tag="P5316_12985"
     CDS             4642..7764
                     /locus_tag="P5316_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRR85917.1"
                     /translation="MSLDNQKMIEEFVVESFEHLADVENDLLTIEEGGADSDPELVNK
                     VFRAVHSIKGAAGFLGLTTINDLAHSLENVLNLVRSKELVPTAPVVDSLLRSVDALRG
                     LLNNVNTSNEQDVRSFIDALKQIISGQASPEVSASLERNVQVGQPATAMFEVSEHLLA
                     THRRLGHQLFVLDFDLIKDVEERGKTPLSLIKDLLALGEVVEARLDAFNLGDLSTGIP
                     DRLRFVALIGTVVDRQMLMVEFGLAEEHIHQVADAGSASPAPAAQAAPAVAAQAVAIP
                     AAPAAPPSSAAPAAPPVSAQDSSPPSETSKTNVETSIRVSVGLLDHLMNLAGELVLSR
                     NQLVQTINNRETRNLESVSARLDQVTSELQEAIMQTRMQPIGTVFNKFPRIVRDLSGK
                     LGKQCDLTIEGKDVDLDKSILETIGDPLTHLIRNSVDHGIEAPQERTSKGKNPIGRIS
                     LRAFHQAGKVNIEISDDGAGIDVNKIKTKAVSKGLISTERAREMTDREALSLIFLPGF
                     SLAEKVTDVSGRGVGMDVVRTNIERLGGTVEIESRVNSGTTIRISLPLTLAIIPSLIV
                     RCGEERFAIPQVNINELVRIKAGDVKSRIEHIKNAEVLRLRGNLLPLVRLSRALGLQS
                     KYLDPVPSELKDDRRKNLADRRADEEHPTEVEARSGQERRQDTTAGALNIIVVETGQL
                     RYGLVVDGLHDSEEIVVKPLGRHMKGVRCLAGATILGDGHVALILDIAGIAAQCAIEM
                     PEEHGATARAEESAAQKAEHQTALLFTNDPSEQFGVSMSLISRIERIRAEQIDSVGGQ
                     EVLQYRGSSLPLLSLDKLIKAKPRPEQPHMYVIVFHLIGQRQEIGLIAPNLVDIREIS
                     TDIDVVTFREPGVLGSLVIDEKTTRLIDLSELARAAHGEWFSQQQQAAATDAGRRILF
                     AEDSAFFRKQVAGFLSEAGYEVQACEDGLVAWNAIQQSAEPFDLVVTDIEMPNMDGYE
                     LTRKIKNDQRFANIPVIAVTSLAGQENIQKGGEVGIDEYQIKLDRDQLIAAVTRLIKR
                     SATAVSGRNA"
     gene            7761..8246
                     /locus_tag="P5316_12990"
     CDS             7761..8246
                     /locus_tag="P5316_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRR85918.1"
                     /translation="MTTTMQRQQRNMAGKELELTTFYVGDLLLGVDIHQVEEINRQLN
                     MTPAPHTPETIRGVVNLRGEVVTVLDLRRALGLEPTIITEDSRNVIVNSRGERVGLLV
                     DRIADVVIAATDQIEPPPANVNGIDGRFFAGVYKLESELVVILDVEEALASEGASKQT
                     T"
     gene            8404..10062
                     /locus_tag="P5316_12995"
     CDS             8404..10062
                     /locus_tag="P5316_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRR85919.1"
                     /translation="MKIGRRLLAGFGVVVAMMVGVITVGYWGLSQVNEKMAEYEKAAA
                     MASKATEVRSTVDGIFFSIVLMSVTSDAAGLERLSKDVETSRASYKRIMENLDQTETN
                     ENGKKLIANIQQKLADARDVNNKVIELAQGGKTAEAAAMAAKDLAGHADSSAQACMDY
                     VAFVGKRQEECNEAAATTSSRVYKMLITIGAAASILAMVFSVLIGRSVSVPIGQVVRQ
                     LDNVAQGDISKNVAQDLVTRRDEVGALSKAIQTMTENLRKTITELAHTSRTLASSSTE
                     LSATATQLASGAEETTGQSATVASAAEELSTNMQNMAASTEEMSNNVKTVAAAVEEMT
                     ASIGEVARNAEQAANVAEQAAHLAATSNQDIGQLGAAADEIGKVIEVIQDIAEQTNLL
                     ALNATIEAARAGEAGKGFAVVATEVKELAKQTAEATEDIRRRIEGIQASTGNAVKSIG
                     EISGVITKVNEVSRTIASSVEEQSITTKEIAQNVGQTATAAQTVSQGVNQSAAASKEI
                     TKNIAGVDQAARQTAQGAAQTQTAGQELSKLAENLQQLVGQFKV"
     gene            10099..11232
                     /locus_tag="P5316_13000"
     CDS             10099..11232
                     /locus_tag="P5316_13000"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRR85920.1"
                     /translation="MNVNASTDRIRVLVVDDTVTYRKVISDLLAEVPEVELLGSASNG
                     RIAIQKIDQLRPDLLTLDLEMPELNGLDVLRHLRATGSTVGAIMLSAFTTQGAEETIA
                     ALSLGAFDFVLKPSTGSIESSIKALRSELVPKIQAFARAAAVKRRLREPAPTRYCPPV
                     LSAPTADVAQRMRKISAMPAGKPEVVAIGISTGGPAALAKMVPALPADLAAPVLIVQH
                     MPPKFTKSLADDLDNRSKLAVSEAVDHQAVEAGRILIAPGGRQMKIERVDGQLVARIT
                     DDPPENNCKPSVDYLFRSVAYTCGRYSLGVVMTGMGNDGTLGCRLLKRAGATIITQDE
                     TTCVVYGMPKAPAEEGLSDVIAPLDRIASEITRFAGKGALACG"
     gene            11223..12077
                     /locus_tag="P5316_13005"
     CDS             11223..12077
                     /locus_tag="P5316_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRR85921.1"
                     /translation="MRVTPDELKILARLVNDLCGVVLDESKAYLIESRLSKVAEDAGC
                     KNFSELYYKARYENNKTIQIKIIDAITTHETLFFRDTSPFEALQHKVFPDLFDAKAKT
                     ATPKRIRIWSAACSTGQEPYSVAIVLHELLPDIATWDIRITGTDISDSAIQQASIGRF
                     RDIEIQRGLKPEILEKYFIREPNSWKIKDEIRAMVAYRRLNLLEPFNGIGPFDVILCR
                     NVAIYFDAVARKSLFERLVKELTPEGYLFVGSSESLTDLGPQFIPQHHCRAIFYQPNK
                     TSLVPVAV"
     gene            complement(12119..12529)
                     /locus_tag="P5316_13010"
     CDS             complement(12119..12529)
                     /locus_tag="P5316_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85922.1"
                     /translation="MQDREKLELFKAALAVAAADRTLKRSELGLIQGLAVKVGIGKAS
                     FDAMMAAALRGEPIATGVCIGSPDTARLALKLLVAEARIDGEISDAERDLLVTIAQRL
                     KIAGDQFQAIYAEGLSQADEIRRRRRLSSQGEKP"
     gene            complement(12574..14931)
                     /locus_tag="P5316_13015"
     CDS             complement(12574..14931)
                     /locus_tag="P5316_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85923.1"
                     /translation="MLGQPPKAVNENTVSWQGKSWHLIDDEGVFACEASVAGERQALP
                     PPNRPAGEIISRRGWDTPVKCRLVDSVDCTQTDHGFEDDGKSRVLDLPGGKFRVTGAA
                     KGFELTWFSYTVQTADRAGVPHLVVVETPNDRERYTTVSLTIPKGEPWSPPFAGQEAV
                     KVNSMEISQEPFWYEPDVGLAVYTGRELPINDKPFTFQYVFFPKAARIRVAVSSSGWA
                     KPNTPESGGAVSRMWVFEILDRLADRLPKIDPPNGRQRRVGLYATHPWYFLAHYGVPP
                     HTPQQRRQSFENMCDLLAFCGMNLIQFNAINGSDRAGRAWYPGSYYRQLGADLLAELP
                     PVAATRGIDIVPVVTSITAPGKIEGGKPNKYGFSELSFQRHADPKADPRAFEHRPPDP
                     LRAETQRWLIRHLLEIGRHCKQHKNVIGIGFRVNGKIGTCYISGEDKSGGETKVYSAE
                     MMGYSSWNLSQFRSESKLAVPNESMEAYQWLKADPKRWDQWLNFRCRHTREFWIKARD
                     AIRSVRPDWTLYVLTDLPSEVTGTNIEWPGADSPDAEKITLDLLRAHGYDPRMFKQDE
                     GIVIQRVMMVDMERFFSKWGPPWGSNPERYRDFHEQEFLPDWFRTPAGSATELYHNYW
                     EEPFHPVGWEFGPDDRGTGMRTSTATARGRAFYRPATFALRVGDTDTIAINGWQRPVM
                     GHEHDLRRFCQAVRALPIGESKPLKTTPLDPKVSATRYADRIAVINDRDKPVTVAVKL
                     DEALPPDKRLRDLATGHVLIEAGTGSRDSFVVQMEDYDLRAMAVE"
BASE COUNT         3083 a         4670 c         4480 g         2764 t
ORIGIN      
        1 gcctggtgca cccggcggcc caagaacctg cgttacctat ttggagataa gaatataact
       61 caattccgag accaggaact ccggttgacc ctctggtggg gcttcggtat tcgcgtagat
      121 cccgtcctga tgccggacgc acacggatcg ccaagccatt tggtttggag caacgaagtc
      181 tttctgcgga ttgtcgccga tgtagagaca ttccggaccc cggcagccgg tcacactctc
      241 aatgtactcg aaggctctct gatgaggttt ccgaaaggct tccccccacc gatcggtcag
      301 cacgagtggt ctcaagagag actccaatcc caacgccctc accttgttct gctgcatcct
      361 gacggggcca tcactaatca atgaaagttg aaacagcccc tgccatttca gaaggacggc
      421 ttctgcgtct ggatacaacc gaatgactgg gttgtggtca cggtagcagg cgatcatctg
      481 tcgaacccag tcctcggcat gtgggcatct ccattcggca agcagttcat cgaacacccg
      541 tcgccgacat ccgctttcga acagctcgcg catccggcgg gccgggtcat cggggcagtc
      601 catctgttgg cgaagccagt tagccaccgc ctcaaacccg ctccagacaa actgccgctc
      661 ggggtaaagg gtatcgtcca gatcaaaaac gaccaccgca attccggtaa gttcgacgaa
      721 ccgctcgctc atcgcattgg attctcggtt cttggcttgt cttggctgat tgtctcgtgc
      781 ttctcgagcg cgtgcccaac ccttggccgc ctttgagccg ctcctggtgc ctcgggattc
      841 ggctctccag ttcgtatcga gggtcggcgc agcgtcagtc cctatccctt acaaagaacg
      901 actgatgaaa gcgaagcatg atctcgccgt cgcggaactg atccagacgg attctcggct
      961 tgcggcccag ccattcggtg agcagccact tcgggaagtc cgcacctgcg tggatcgcca
     1021 aaggcacccc gccgccgaat cgagggttga tctcgatcac ccggatcctg ccgtctggag
     1081 aaagaatcag ttggatcgtg atcagcccca cgcacccggc caatgcctcc accacgcgta
     1141 cgccggtcgc catgatccgc cggttcttaa ccgtgcgggc cttagtgact tcgcccccgc
     1201 ggacctcgat gcgctcgcgg ggaacaacgc accgggggca accgtcgaaa cccgcgtaca
     1261 cgtcaagcgt gtgctccagc ccgggcacaa actcctgaac gatcgcctct ggcactctgg
     1321 gtaccatggc ttccagatcc cgccgatggt ggatcacgaa acttcccctg ctcgcgcttc
     1381 cacgcctggg tttcaggaaa tagggaaagc gatggcgccg ccgcgccaga gcctcctgcg
     1441 cagtccacgt ggccggcgtg tcgatccggt ggctcgtcag aaatcggtac gtcaacagct
     1501 tgtcgcgaca ggtatgcaca acggccggag atgaaatcag ggcaatacac gacgatcggg
     1561 cgaactcatc tcgagactcg gcgagcttga ccagatccgt atccgtgagc ggaacgagaa
     1621 tgtcgatccg ccggcgcttg acgattctca gtaatgaacg gatatagtcc gtcgagtgag
     1681 tcggaggaac ctggtgagcc tcatcggcaa tacaagcagc cgcaaaatgg ctctcagtat
     1741 ccgccacgtg gatactcgga cgaagcccga gcgccctggc tgctcgtata aacgcactga
     1801 tcaactcgat tcgcctgccg gcgcaagtga atagtatccc cacatctcgg cggcgcgcgc
     1861 cggctccgta catggcttgt attccggtca tccagtgccc gtcatatcga cttcgtcagt
     1921 tcaacttggg cctataggtt gacgcccctt cgaccggccg tcaacctgcc cagccccggg
     1981 ccgttggacg agtgtccaat aatcctggcc ggcggctcaa ggcacaccct gccattggct
     2041 attcatcgac aagacaaaca ggacacacaa gtcctgggca aacccgcgtt ggccggccta
     2101 ctcaggccac gcgtactgca ggatcccata gcgccataac cggccgcaag gctgcaaacc
     2161 acaggcagca aggtctcgcg gacctgtgcg gttaccggac taaacgcggc ttccgactgg
     2221 gtgccaagtg aaccctttcg ccggcgttgt tgagctcatc aggatgagca ttttcccgtg
     2281 atttcccgat ccgatggcaa gtaaaggcct tgtttccggg ggtcgataca ccagtcgtct
     2341 ggctgaatct tccccgggtt atggctcggc gtggggtgcc gtccggacca cgacgtctgg
     2401 ataacaccat cgatccgtca gcggccatca cagtgtcttt cccgacacgg cttgggagat
     2461 acccggggtt gcggcttggc acagcgcacg gaggcacagg tgggcggcaa ggccgccgcg
     2521 cagtctatgt gttcgatctg ccctcaggag acgggttgtg gctacctcgc ccacagaggc
     2581 cgaactgctc cgctgtgccg tcccgacgcc aagcagcggc gccgagcggg acgcgacacc
     2641 tgaagtcgac gccctgggca tgctcggatc ccaacccggc actgcgtcca ttctggtggc
     2701 tgatgacgac ccggacgtgc gggcgttgat cagacatctt ctcaaaaagg ccggatacga
     2761 ggtccttgaa gccgaggatg gagagcaggt tctcgccatg ctggagaagc gtctgccgga
     2821 gctgatcctg ctggactgcc agatgccccg tctcgacgga ttcagcacct gcctcgaatt
     2881 gaagaagaat cccaactatg cggatctgcc agtggtcttc ctgacggtcc gctccgatcc
     2941 cagcgacaag gcccggggct ttgcggtcgg cggcgaagac tacatcacca agcctattga
     3001 acgacaggag ttcctcgcgc gcattcgaag ccgattggag ctgtcgctga atcgccgatt
     3061 gcttcgccgc aaggcaacga tctacgaagc cgtgtccatg gagagcgccg accgccttga
     3121 ggatgttcgc gacggacagg tgtctttgtt gacaccgccc agcagcctgc cggaactgaa
     3181 actgggcgtc cggtttcaac cggcccatga agccggcgga gatttctacg agatcgcccg
     3241 tctgtgcgat gatgagttcg ggtttctggt cagtgacgtt tccggccatg acctgagcgt
     3301 cccgttcgtt acgggggccc tcaaagctct ggcggccacg ttcctgaacg aatcgctgac
     3361 ccccctcgag gtcttgtcat tgcacaactc aagtctcctc aagtttctga gcgagggacg
     3421 ctatgtcacc gcctgctgtg cgcgcttctc gaagctgacc atggaggtgg aggttgccaa
     3481 tgccgcccac ccgccggcgt tctttcagtc atcgcgccat gagggcgagt tcatcgatct
     3541 cagcggtgac attctgggca tgctggagac cgccaggttc gagtcaaaac gctttccggt
     3601 tgaacggggc gaacgactgt ttctctacac cgacgggttg atcgaaagcc ggcgaggtcc
     3661 cgatgggcgc ttaagcgtgg tccaaggcat ggcctggttg aaggaaggtc tgctcgcccg
     3721 gcgcgccctg ccgatcgatc aagttgtgga ttcggtggtg gacgaactgt tgtgtgattg
     3781 cggcgggaag gcggaagacg acgtcgttct gatgggaatc gagttttgag gccggacatg
     3841 ttcgacatcg agcatactcc agacgcgttg cggatcacga tgtccccgtc gttcgagaac
     3901 atcgaccggg ccgatgatct ttgcgcccag gaactcgacc ggcgtcaggt ccctgtcaac
     3961 atgttcgcgg tgcgtatcct cctccgcgag gcggccctga acgcggtgac acacggatgt
     4021 cgcgaggcgc ccgaaggaca ggtccgcgtg gcactggtct tcgcaacgga cgaaatcaaa
     4081 ctcatcgtgg aagacaacgg tcccgggttt gtctgggaaa accgcgaagc gggattcgac
     4141 gtgacgggtg acggcggcag gggactgccg ctgatgaaaa catacgcttc acaagtgacc
     4201 ttcaacgagg ccggcaaccg ggtcgaactg agcgttcgcc atgagctcac cccgccggtc
     4261 gggtgtgtta tctgagagaa tgggagaaca gcatgacatc tgtaacggaa acacgacaaa
     4321 cgcacaccgt ccgtccggac tacgatatcg tcgcctcgca cacggaacag tttcgcgcgc
     4381 agcttcagga tctgatcagc cagggtcacg ttcacctcgt gatcgacctg ggcgacgtga
     4441 aaatgatcga ttccaagggc ctggcggtcc tgatgctctg tcacaagagt gtaagcgccg
     4501 ccggaggttc gcttacggtc ttaacgcgca atcaggactt caggcatttg ttccatgtca
     4561 tgcgcatgga cgagcacttt acgatcgccg agagcctgtg atactgctga attcgatggg
     4621 agaactggtc ggagtcacaa catgtccctt gataaccaga agatgattga agagttcgtc
     4681 gtcgagtctt tcgaacacct ggccgacgtc gagaatgatc tgcttaccat tgaagagggc
     4741 ggcgcggaca gcgaccccga attggtcaac aaggtattcc gggccgtcca ttcgatcaag
     4801 ggggccgccg ggttcctcgg gctgaccacg atcaacgatc tggcgcacag cctggagaac
     4861 gttctgaacc ttgtccgttc gaaggaactg gttccgaccg cgccggtggt cgattcgctt
     4921 ctgcgatcgg tggacgcgct tcgcgggctg ctgaacaacg tgaacacctc caatgagcag
     4981 gatgtccgat ccttcatcga cgcactgaag cagatcatca gcggccaggc gtctcccgag
     5041 gtctcggcca gcctcgagcg caacgtacag gtcggacaac cggccaccgc catgttcgag
     5101 gtgtcggagc accttctggc aacccaccgg cgactgggtc accagctctt cgtgcttgac
     5161 ttcgacctga tcaaggatgt cgaggaacgg ggcaagacgc ccttgtcgct catcaaggat
     5221 cttctggccc tcggggaagt ggtcgaggcg aggctcgatg cgttcaatct cggggatctc
     5281 tccaccggca tacccgacag gctgcgtttt gtcgccttga tcggcacggt ggtcgatcgc
     5341 caaatgctga tggtggagtt tggtctggcg gaagagcaca tccaccaggt cgcagatgcg
     5401 ggttcggcat ctcccgcccc tgcggcgcaa gctgcccccg cagttgcagc ccaggccgtc
     5461 gcgatcccgg ccgcacctgc cgcgcctcct tcgtcggccg cgcccgccgc gccgccggtc
     5521 agcgcccagg actcttcgcc gccttcggaa acgtccaaaa caaatgtcga gaccagcatt
     5581 cgcgtatcgg tcggcctctt ggaccatcta atgaacctgg ccggcgaact ggttctctcc
     5641 cgcaaccaac tcgtccagac gatcaacaat agggagacgc ggaacctcga gtccgtcagc
     5701 gcccgcctcg accaggtcac cagcgagttg caggaagcga tcatgcagac ccgcatgcag
     5761 ccgatcggga cggttttcaa caagttcccg agaatcgtcc gcgatttgag cggtaagctc
     5821 ggcaagcagt gcgacctgac catcgagggc aaggatgtcg acctcgacaa gtccattctc
     5881 gagaccattg gcgacccatt gacccacctg attcgcaact ccgtcgacca cggcatcgag
     5941 gcgccacagg agagaacctc caagggcaag aaccccatag ggcgcatttc tctccgggcc
     6001 ttccatcagg ccggcaaggt caacatcgag atttccgacg acggtgcggg catcgacgtg
     6061 aacaagatca agaccaaggc cgtcagcaag ggcttgatca gcaccgaacg agcccgggaa
     6121 atgaccgacc gcgaggcgct gagtctgatc ttcctgcccg gcttctcgct ggcggagaag
     6181 gtcacggacg tcagcggccg aggcgtgggc atggacgtgg tccgtaccaa catcgagaga
     6241 ctcggcggaa cggtggagat cgaaagccgc gtgaactcgg gcaccaccat tagaatcagc
     6301 ctgccgctca cgctggccat cattccgtcg ctgatcgtgc gttgcggaga agagcgtttc
     6361 gccatcccgc aggttaacat caacgaactc gttcgcatca aggccggtga cgtcaagagt
     6421 cgcatcgagc acattaagaa cgccgaggtg ctccggcttc gcggcaatct cctgccgctg
     6481 gttcgcctca gccgcgccct cggtctccag tccaagtacc tggacccggt gccatccgaa
     6541 ctgaaggatg accgccggaa gaacctcgcg gaccggcgtg ccgacgagga acacccaacc
     6601 gaggtcgaag cccgcagcgg tcaggagcgc cggcaggaca cgacggccgg agccctcaac
     6661 atcatcgtgg tggaaaccgg ccaactcaga tacggactgg tggtcgacgg cttgcatgat
     6721 tccgaggaga tcgtcgtcaa accgctcggg cgtcacatga aaggcgtccg ttgcctggcg
     6781 ggggcgacca tactgggtga cggtcacgtg gccctgatcc tcgacatcgc cgggatcgcg
     6841 gcgcaatgcg cgatcgaaat gccggaggaa cacggtgcaa cggcgcgtgc cgaggaatca
     6901 gccgcgcaaa aagccgagca tcaaacggca ttgctcttta ccaacgatcc atcagagcaa
     6961 ttcggcgtgt ccatgagtct catctcgcgg attgagcgca tccgcgccga gcagatcgac
     7021 agcgtgggcg gccaggaggt cctgcaatac cgggggagtt ctttgccgct gctcagtctg
     7081 gacaagctga tcaaggccaa gccgcggcct gagcagccgc acatgtacgt cattgtcttc
     7141 catctgatcg gccaaaggca ggagatcggc ctgatcgccc cgaacctcgt ggatatccgg
     7201 gagatcagca ccgatatcga cgtggtgacg ttccgcgaac ccggagtgct cggctcactg
     7261 gtgatcgacg aaaagaccac tcgcctgatc gacttgtccg agctggcccg agccgctcac
     7321 ggcgagtggt tcagccaaca gcagcaggcc gcggcgaccg acgccggccg tcggatcctg
     7381 ttcgcggaag actcggcctt cttccgcaaa caggtcgccg gtttcctgtc cgaggccggc
     7441 tatgaagtcc aggcctgcga ggacggtctg gtcgcctgga atgccataca acagagtgca
     7501 gagccgttcg acctcgtcgt cacggacatt gaaatgccca atatggacgg ttacgagctg
     7561 accagaaaga tcaagaacga tcaaaggttc gccaacatac cggtgatcgc ggtgacttcg
     7621 ctggccggac aagaaaacat acagaaaggc ggcgaggtcg gtattgatga gtatcagatc
     7681 aagctcgatc gcgaccaatt gatcgccgcg gtaacccggc tcatcaagag gtcggctacg
     7741 gcggttagcg ggaggaacgc atgacaacca ccatgcaaag acaacagaga aacatggcgg
     7801 gcaaggagct ggagttgacc acgttctacg tgggtgacct cctcctcgga gtcgacatcc
     7861 atcaggtcga ggagatcaac cgtcagctca atatgacccc agccccccac acgcccgaga
     7921 cgatccgggg ggtggtgaac ctccgcggcg aggtcgtcac cgtcctcgat ctgcgccggg
     7981 cgctcgggtt ggaaccgacg attatcaccg aagacagcag gaacgtcatc gtcaactcgc
     8041 gcggtgagcg cgtgggcctg ctggtcgacc ggatcgccga cgtcgtgatc gcggccaccg
     8101 atcagattga gcctccgccc gcgaatgtca acggcatcga cggccgcttt ttcgcgggtg
     8161 tatacaagct ggagtcggag ctcgtggtca ttcttgacgt ggaagaggcg ctcgccagcg
     8221 agggggcctc caagcagacg acgtgaaccg tgtgaaaggt tagcacctta cggccagaga
     8281 gccgtgcggg ttgcgcgcat gttttcggat cgctgtacgt tcgggttgcg gcccgcacaa
     8341 agacggaccg ccttgggttc agagagtctt ttttgaggat caggtgccac tatgttcaag
     8401 aacatgaaaa tcggaagacg gttgctggcc gggtttggcg ttgtggtggc gatgatggtc
     8461 ggcgtcatca cggtcgggta ctggggactc agtcaggtta acgagaaaat ggccgagtac
     8521 gagaaagccg ctgcgatggc ttccaaggcg acggaggtgc gaagcaccgt tgatggcatt
     8581 ttcttctcca tcgttttgat gtcggtgacc tccgatgccg ccggccttga gagactgtcg
     8641 aaagacgtgg agacgtcgag agcgtcgtac aagaggatca tggagaacct tgatcagacg
     8701 gaaacaaacg agaacggcaa gaaactgatt gccaatattc agcagaagct ggcggatgcc
     8761 agggacgtca acaacaaagt cattgaactg gcccagggag gcaagaccgc cgaagccgcc
     8821 gccatggctg caaaagacct ggccggacac gcggactcgt ccgcgcaggc ctgcatggac
     8881 tacgtcgcgt tcgtcggcaa gcgtcaggaa gagtgcaacg aggcagccgc gacgacttct
     8941 tcgagagtct ataagatgct cattaccatc ggcgccgcgg ccagcattct ggcaatggtt
     9001 ttcagcgtcc tgattgggag aagtgtatcg gtgccgatcg gccaggtggt tcgtcagctt
     9061 gataacgtgg cccaaggcga catttccaag aacgtggctc aagatctcgt aacccgtcgc
     9121 gacgaagtcg gcgcgttgtc caaggccatt caaacgatga ccgagaacct ccgtaagacg
     9181 atcaccgagc ttgcgcacac ttctcggact cttgccagtt cgtccacgga actatcggca
     9241 acggccacgc aactcgccag cggggccgag gagacgaccg ggcaatccgc caccgtcgcc
     9301 agtgcggcgg aggaactgtc gaccaatatg cagaacatgg cagcctccac cgaggaaatg
     9361 tccaacaacg tgaagacggt ggctgcggcg gttgaagaaa tgaccgccag catcggcgag
     9421 gttgcccgga atgccgagca ggccgcgaac gtcgcggagc aagccgccca tttggccgcg
     9481 acgagcaatc aggatatcgg acaactcggc gccgccgccg acgagatcgg aaaggtcatt
     9541 gaggtcattc aggatatcgc cgaacagacg aacttgcttg cccttaacgc cacgatcgag
     9601 gcggcccgcg ccggtgaagc cggcaaaggc tttgcggtcg tcgccactga ggtcaaggaa
     9661 ctcgccaaac agaccgcaga agccaccgag gacatccgca ggcgaatcga gggcatccag
     9721 gcttcgacgg gcaacgcggt gaagtccatc ggcgaaatca gcggcgtgat caccaaggtc
     9781 aacgaggtgt ctcgcacgat cgcctcttcc gtcgaagagc aaagcatcac caccaaggag
     9841 atcgctcaga acgtcggaca gacggcgacg gcggcacaga ctgtttcgca aggcgtcaac
     9901 caatcggccg cggcgagcaa ggaaatcacc aagaacatcg ccggtgtgga tcaggctgca
     9961 cggcaaaccg cgcagggcgc agcccagacc caaacggccg gccaggagct gtccaaactg
    10021 gcggagaacc tccagcaact ggtagggcag ttcaaggtgt aaggaacctc gcggcggggg
    10081 tggcgagtga gaggcgactt gaacgtcaac gcaagcacag acaggatccg ggtgttggtc
    10141 gtcgacgaca ccgtcaccta ccggaaggtc ataagcgacc ttctggccga ggtgccggag
    10201 gtcgagctct taggctctgc ctcgaacggc cggatcgcca tccagaaaat cgatcaactg
    10261 cggcccgacc ttttgaccct ggatctggag atgcccgagc tgaacggtct cgacgtcctg
    10321 cgccatctcc gcgcgaccgg cagcacggtg ggcgcgatca tgctgagcgc ctttaccaca
    10381 cagggtgccg aagaaacgat cgccgccctg agtctggggg catttgattt cgtgttgaag
    10441 ccgtcgaccg gcagcatcga gagcagcatc aaggcgctgc gttcagaatt ggttccgaaa
    10501 atccaggcat tcgctcgtgc cgctgcggtc aagcggcgac tgagagagcc ggccccgaca
    10561 cggtactgtc cgcccgtcct ttccgctccg acagccgacg tcgctcagcg gatgcgcaaa
    10621 atctcggcca tgccggccgg gaagccggaa gtcgtcgcca tcggcatttc cacgggaggt
    10681 cccgcggccc tcgcgaaaat ggtgccggca ttgcccgcag acctggccgc gcccgtgctg
    10741 atcgtccagc acatgccgcc gaagttcacc aaatcgctcg ccgacgacct cgacaatcgc
    10801 agcaaactcg cggtcagcga ggccgtcgac caccaagccg ttgaggccgg gcgtattctc
    10861 attgcacccg gcggccgcca gatgaagatc gaacgcgtcg acggtcagct cgtggctcga
    10921 atcaccgacg atccgccgga aaacaactgc aagccgtccg tggactacct gttccgctca
    10981 gtcgcctaca cctgtggacg ttactcattg ggcgtggtca tgacggggat gggcaacgac
    11041 ggcactctgg gctgtcgatt gctcaagcgc gccggcgcaa ccatcatcac ccaggacgag
    11101 acgacctgcg tcgtgtacgg catgcccaag gctccagccg aagaaggcct gtcagacgtg
    11161 atcgccccgc tggaccggat cgcgtctgaa attacccgct ttgccgggaa aggagcattg
    11221 gcatgcgggt gacgccggac gaactcaaaa tcctcgcccg tctcgtcaac gacctttgcg
    11281 gagtggtcct tgatgaaagc aaggcctatc tgatcgagag ccgcctgtcc aaggttgctg
    11341 aagacgcagg ttgcaagaac ttctcggaac tgtactacaa ggcgcgttac gagaacaaca
    11401 agaccattca gatcaagatc attgacgcca tcaccaccca cgagactctc tttttccgcg
    11461 acacgtcacc ctttgaggcc ctgcagcaca aagtgtttcc cgatctgttc gatgccaaag
    11521 cgaagaccgc cacgcccaag cgaattcgga tctggtcggc cgcctgcagc accggtcaag
    11581 aaccctacag tgtggcaatc gtgctgcacg aactcctgcc cgatatcgcc acctgggaca
    11641 tcaggatcac cgggaccgac atctcggact ccgcgattca acaggccagc atcggcagat
    11701 tccgcgatat cgagattcaa cgcggcctca agccggagat ccttgagaaa tacttcatcc
    11761 gcgaaccgaa cagttggaag atcaaggacg agatccgcgc gatggtcgca tatcgccgct
    11821 tgaatctgct cgaacccttt aacggcatcg gaccgttcga tgtgattctc tgccggaacg
    11881 tggccatcta ctttgatgcc gtggcccgca aatccctttt cgagcgactg gtcaaggagc
    11941 tgaccccgga gggatatctg ttcgtcggat catccgagtc gctcaccgat ctcggcccgc
    12001 aattcattcc ccagcatcat tgccgcgcca tcttctatca acccaacaag acgtcgcttg
    12061 ttcccgtcgc ggtttgaggt gtccgcgcca ccgcctgccc gccgggcagg cctccctctt
    12121 acggtttttc tccctgactc gacagacggc ggcggcggcg aatctcgtcg gcctggctga
    12181 gaccttcggc atagatcgcc tggaactggt cgccagcgat cttgagccgc tgggcgatgg
    12241 tgacgagcag atcgcgttcg gcgtcgctga tctcgccgtc tatgcgggcc tcggcgacga
    12301 gcagcttgag tgccagtcgt gccgtgtccg gcgacccgat acatacgccc gtggcaatgg
    12361 gctcaccgcg caaagcagcc gccatcatgg cgtcaaaaga cgccttcccg atgccgacct
    12421 tgacggccag cccctggatc aggcccagct cagaccgctt gagcgtccgg tccgccgctg
    12481 caaccgccaa cgccgccttg aacagctcca gcttctctcg atcctgcacg atcgtctccc
    12541 tgccgccgcg tgacgaaccg gtcaaccgcc ctcttattca accgccatcg cacgaagatc
    12601 gtagtcttcc atctgaacga cgaaagagtc ccggctgcct gttcccgctt caatcagcac
    12661 atgccccgtg gccagatctc gcaaccgctt gtccggcggc aaagcctcat caagcttcac
    12721 cgcaaccgtc acgggcttgt cgcgatcgtt gatcaccgcg atgcggtctg cgtaccgggt
    12781 agccgacacc ttcgggtcga gaggcgttgt tttcaacggt tttgattcgc cgatcggcaa
    12841 ggccctgacc gcctggcaga accggcgcag gtcatgttcg tgccccatga cgggccgttg
    12901 ccagccgtta atggcgatcg tgtcggtgtc accgacacga agcgcaaagg tcgccggtcg
    12961 atagaaggcg cgccccctgg ccgtggccgt tgaggtccgc ataccggttc cgcgatcgtc
    13021 ggggccaaac tcccagccca ccggatggaa cggttcttcc cagtagttgt gatacaactc
    13081 ggttgccgac ccggccggcg tccggaacca gtccggcagg aactcctgtt cgtgaaaatc
    13141 gcgatatcgt tcggggttgc tgccccacgg ggggccccac ttgctgaaga accgctccat
    13201 gtccaccatc atcacccgtt ggatcacgat cccctcgtcc tgtttgaaca tccgcgggtc
    13261 gtagccgtgg gcccgcaaca agtccagtgt gatcttctcc gcatccggcg agtccgcgcc
    13321 cggccactcg atgtttgtcc ccgtaacctc cgacggcagg tcggtgagca catacagcgt
    13381 ccagtccggc cgaacgctgc gaatcgcgtc acgggcctta atccagaact cccgcgtgtg
    13441 ccgacagcga aagttgagcc actggtccca ccgtttcgga tccgccttga gccactggta
    13501 ggcttccatg gactcattgg gcaccgccag cttcgactcg ctgcgaaact ggctcaggtt
    13561 ccacgacgag tagcccatca tctcggcgct gtacaccttc gtctcgccgc cggacttgtc
    13621 ctcgccggag atatagcagg taccaatctt gccgttgacg cgaaagccga tgccgatcac
    13681 attcttgtgc tgcttgcagt gccggccgat ctccagcagg tgcctgatga gccatcgctg
    13741 cgtctcggcg cggagcgggt ccggcggccg atgctcaaac gcccgtggat cggccttcgg
    13801 gtcggcatgt ctttgaaaag acaactccga aaacccgtat ttgttcggtt tgccgccttc
    13861 gatcttaccg ggagcggtga tcgaggtcac gaccgggacg atatcgatgc cccgggttgc
    13921 ggcgacgggt ggcagttcgg cgagcagatc ggccccaagc tgccgatagt agctgcccgg
    13981 gtaccacgca cggccggccc ggtccgaccc gttgatggca ttgaactgaa tcaggttcat
    14041 gccgcagaag gcgagcaggt cgcacatgtt ctcgaacgac tgccgccgct gctggggcgt
    14101 gtgcggcggc acgccgtaat gggccaggaa gtaccagggg tgcgtggcgt agagcccgac
    14161 gcgacgctgc cgcccgttcg gcgggtcgat ctttggcaac cggtcagcca accggtcgag
    14221 aatctcgaag acccacatcc gcgagaccgc cccaccgctc tcgggagtgt tgggtttggc
    14281 ccagccgctt gagctgacgg ccacacggat cctcgccgcc ttcggaaaaa agacgtactg
    14341 aaacgtgaag ggcttgtcgt tgatcggcag ctcgcggccg gtatagacgg ccagacccac
    14401 gtccggctca taccagaagg gctcctgcga aatctccatc gaattgacct tgacagcctc
    14461 ctggcccgca aacgggggcg accagggttc acccttcgga atagtcagag acacggtcgt
    14521 atagcgctcg cggtcgttgg gggtctcgac gacgacgagg tgcggcacgc ccgcccggtc
    14581 tgcggtctgc accgtgtaag agaaccacgt cagctcgaag cccttggccg caccggtcac
    14641 ccggaacttg ccgccgggga ggtcgagcac ccgcgacttc ccgtcgtctt caaaaccgtg
    14701 gtcggtttgg gtacagtcga cggagtcaac cagccggcat ttgacgggtg tatcccagcc
    14761 gcgtcggctg atgatttctc cggccggccg attgggcggc ggcaaggcct gacgttcccc
    14821 tgcgaccgac gcctcacagg caaacacccc ctcgtcatcg atcaaatgcc agctcttgcc
    14881 ctgccatgac accgtgtttt cattcacggc cttgggcggc tggccgagca ccaagggact
    14941 gcgtaaccgt tcagacccct atagcgacca gcgctggcgc cggctgaccg gtgcgat
//
LOCUS       DAOTPW010000136        14954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3925_length_14954_cov_5.229948, whole genome shotgun sequence.
ACCESSION   DAOTPW010000136 DAOTPW010000000
VERSION     DAOTPW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14954
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3925_length_14954_cov_5.229948"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="P5316_13020"
     CDS             <1..1421
                     /locus_tag="P5316_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRR85924.1"
                     /translation="PSRGCMGGSRCMTVAAAQISARSGEEAELTLASLDSAIRKAHRA
                     GAHLLVLPECAYPAYLLGSVESYRAGRYMPGEDFVRWLAGRAADCRMHIVSGFVDDGG
                     NRLHNAAVLLDDHGREIGRTHKTFLWHLEHDWYTAGDQIRTFDTQLGRIGMIICAETR
                     SPEILATLAADGAELLAMPTCWVNSARQAGEFHNPQVEFLIEARAREFGLPIVCADKW
                     GVELPGFGYVGQSRIVRADGSLAAEAGPTGEAVICAQITPAKSNRQADPREHTARLLS
                     QSAPVRLRASDLLPLRVALMTAFVRPRALAANGPVLRHLQQHKPDLLVAQAANIDDER
                     NLRRVAADLGVRLLLPPPACQMIDLAAGRAACLVGPAARSFATPRSMVLDGAAMLIFW
                     EEPSDLPILRARALENRVFVIAINTSFVAIIGPDGTILTHTSSESTLPPVVVLHLGEA
                     ADKLVAPNTDVFSERRPATYRF"
     gene            complement(1908..2807)
                     /locus_tag="P5316_13025"
     CDS             complement(1908..2807)
                     /locus_tag="P5316_13025"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRR85925.1"
                     /translation="MENERIILQTNIPGFPVRRGKVRDIYDLGDKLLIVASDRISAFD
                     VVMPNGIPFKGEVLARISRFWFDFVKGIRHHLIEFIDEKAPPGFEPYLDQLAGRSMLV
                     KKTKVLPIECVVRGYLAGSGWKEYQKTQSVCGIKLPPGLRQCDKLPEPLFTPATKAEE
                     GHDENVSFERACQLTDPKTMETVRRLSISVYTQAAEYARRRGVIIADTKFEWGFDGEE
                     LILIDEVLTPDSSRFWPADQYEPGRDQPSFDKQFVRNYLETLNWNKTPPGPALPTDVI
                     LGTARKYIEAYERITGQEFPKSL"
     gene            complement(2951..4333)
                     /locus_tag="P5316_13030"
     CDS             complement(2951..4333)
                     /locus_tag="P5316_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85926.1"
                     /translation="MNTRRFLMTGLLVLSTAVSTRADAVIMYNGQRLEGQIVNETEDE
                     VWLRQSRPDGIAFVRQIHRFNIARVERGGPSQSQPSSAPAEIEAPPIQPDVEKLEQLD
                     LVISKYQDRNFEWAGFLVTQLIAKSSPGELTYMSAEVQKRLKMSLAELAADSHFKSAE
                     PAKPGERMRLPYVTHYERPALLKLLTRAHEEALGRSIGGGKNEFKSVAPQALPPGQLK
                     TQTDDPRYMTVDRRPEDASDTAVRPVPPQADLPSAATEREPNEPAPAGSPATNSNSSA
                     KRPSGSLGGGLSSLRPTSTLAPSSGSPKATASRPCPWPFQRPTTAVDWLDHPEDYDGT
                     PAEAEAMAKHLQYTISLLSERIRLDPKARTDSVFRTALIQEKDRLGLLLKAVKAQAKG
                     NLTQKQRAAILAQRKRLEESHRKEVVPRELLIEEYVRQGLKDGPPPGKYPTTCPGGNV
                     ELVPVDISTP"
     gene            4658..4810
                     /locus_tag="P5316_13035"
     CDS             4658..4810
                     /locus_tag="P5316_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85927.1"
                     /translation="MSFIHLCRLNDFDPFDFLTELQRHAATPAARPENWMPWNRREGL
                     GATSPA"
     gene            complement(4897..5268)
                     /locus_tag="P5316_13040"
     CDS             complement(4897..5268)
                     /locus_tag="P5316_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85928.1"
                     /translation="MSKHKSSFVLPWKPILYGLLALVGGVVLLLLSRYIKHKPLTSQP
                     AAVTASAPAPATQPGPASPDRPLRRSRPEAAASGLSSLILLFSMACFMVFLICVGWVV
                     VEIRNARPAWQRQTKYPKMRE"
     gene            complement(5349..5813)
                     /locus_tag="P5316_13045"
     CDS             complement(5349..5813)
                     /locus_tag="P5316_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRR85929.1"
                     /translation="MIRNAMMKDVPEIHRLVTYHAELNRMLFRSYADLYEHLRDFLVW
                     VECGSEGEQVRGCVAVELVWRDLAEVKSLAVEESYRGRGIGRHLVGAAIEEGRRLALK
                     RLFALTREKVFFEKLGFAVVPKETLPHKVWTDCIRCPLQEHCDEVAMVYELS"
     gene            6177..7577
                     /locus_tag="P5316_13050"
     CDS             6177..7577
                     /locus_tag="P5316_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRR85930.1"
                     /translation="MDLFSQQRKDAFNRVQPLAVSMRPRTLEEFVGQEHFLGPGKLLR
                     RLLAADRLSSAIFYGPPGTGKTTLAHIIAGKTQAAFIEVNAAAVGVKEVRGILEEAKE
                     RLASTGKRTVLFLDEIHRFNRAQQDILLNDVENGLIILIGATTENPFFTVNSPLVSRS
                     QIFQFEPLTKEHIRILLRRAVADPERGLGRYKVEIQQEAIEHWATTCDGDARRALLAM
                     EVAVLSQIDSTTSEASNPIVVDLKVAEESIQRKAITYDGSGDEHYDAASALIKSMRGS
                     DPDAAVYWLARMLEAGEDPRFIARRVAICAAEDVGNADPHALVVANAAAQVTLLVGMP
                     ECQLVLSQAVIYIACAPKSNASAAAIWTATKDVREARTIPVPKHLRDSHYRGAARMGH
                     GTGYKYAHDYQGGFVEQDYLGVDKTYYTPTDRGHEAVIRAYLASLANQTEKRPGGPLG
                     GSKENRPREIGTDDSR"
     gene            7574..8173
                     /locus_tag="P5316_13055"
     CDS             7574..8173
                     /locus_tag="P5316_13055"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRR85931.1"
                     /translation="MKPATTPDHDASKKELRQRLRQVLDGISSEELSHRSHLACNKLI
                     ATSVYEQSDVIMVFLSLPREVDTTPLVLHAWRDGKRVLAPKVNWDQRRMLPIEIVSLT
                     DNIIGGAMGLREPVEGTPFPVEQIDLVVVPGLGFDLKGNRIGRGRGFYDRFLKHEGWK
                     GTSCGLALEDQVVEHVPMSEHDATVDMLVTDAAVRVFRK"
     gene            8238..9299
                     /locus_tag="P5316_13060"
     CDS             8238..9299
                     /locus_tag="P5316_13060"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRR85932.1"
                     /translation="MAYGRRRRRKSRFMSRFIILLLIAGGGYYAYVKYYKKPGAPGPE
                     STATAPSDVRTQPPGGGVLTPPLEEQFGGIAGSQPASQPTASRPFWATVQPFEKEPIA
                     VPPADQIEAVYQEGMQAWQAGDLLKARDALNRTLHGDIPVERQAQIREILNQIAEKTI
                     FSLDLLPGETLAEDYVVQQGDMMQKIGRQYKITEELISSINGGMNKNFIRAGRRLKVI
                     KGPFHATVVKSSFEMHLYLQNVYVRTYKVALGADNSTPSGKWVVTERLTNPTWTNPRT
                     GQRIAADDPQNPLGEYWIALEGIEGRAMGQSGYGIHGTIDDSSIGTQASMGCVRLSSK
                     DIEVVYSMLVPGSTVTITE"
     gene            complement(9361..11112)
                     /locus_tag="P5316_13065"
     CDS             complement(9361..11112)
                     /locus_tag="P5316_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRR85933.1"
                     /translation="MANFFLDNEDLQFLFNHLDLAEIAAVQEDNFTRDRGNGYEYAPV
                     DAADAIDNYRRVLTLVGEIAGDHIAPRAEKIDHEGNRLNPDGTVTLNENVRENLNVLA
                     KADLMGFTLPRRYGGLNCPCLIYTMAIEIVSRADASLMNIFGLQGIAETIYAFAGEDI
                     RSEYLPRFAKGEVTGAMVLTEPDAGSDLQSVSLRAYQDEDGQWRLDGVKRFITNGCGE
                     VLLTLARSEPEISDGRGLSLFLAERGPQIRVRHLEEKLGIHGSPTCELVFQNVPARLI
                     GERQRGLITYVMSLMNGARVGIAGQSLGIAEAAYRVAREYAHSRKQFGVPIEQLPAVA
                     EMVTDMKVHIEAARALVYETCRVCDFENNNIRILDYFTPTDPEELKRRKADARTYKRL
                     NGMLTPMSKYYASEMCIKVANDALQVLGGSGYMKDYPVERYLRDARITTIYEGTSQLQ
                     IVAAVRGVCSGVFEKQAAEYETRQYSDSLLNELKARLLEARELVLKGVAFVKTQSNEY
                     MDLSGRRLVDSAVAVLCGHLLLRQAENNERKRRVARRFIMTSLPSIRRDIELVCSGDR
                     SALDEYQTLAGPVPVPK"
     gene            complement(11115..12146)
                     /locus_tag="P5316_13070"
     CDS             complement(11115..12146)
                     /locus_tag="P5316_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRR85934.1"
                     /translation="MIEPNRKGEVWVFAEQEDGNLSEVSLELCGKAREMADRLKVKMG
                     AVLPGYRVRDLSNRLIAHGADNVYCVDDPQLEYYRTLPYARVICELINKHKPQIVLYG
                     ATALGRDLAPRVASEMKAGLTADCTDLDIGDYTDPKSKETFQDLLLQIRPAFGGNIIA
                     TIVNPRRWPQMATVREGVMRLADGNPKRTGDLILEKVSLDGVTGVVKIVERHREKQKV
                     NLKGARVIVAGGGGVGSKENFQLIYDLAGVLGAAVGASRAAVDSGLIGKEHQIGQTGT
                     TVRPALYVACGISGAIQHRAGMEESAKIIAINKDANAPIFSVAHYGIVGDLNKVIPMM
                     IKAIKERAR"
     gene            complement(12247..13161)
                     /locus_tag="P5316_13075"
     CDS             complement(12247..13161)
                     /locus_tag="P5316_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRR85935.1"
                     /translation="MSYDCVVCVKQVPDTANITGEAMKADGTVNRAALPAIFNPEDLH
                     ALEAALGVRETHGGTVTAVTMGPPTACEVLRDCLYRGADRAILVTDRRAAASDTLATS
                     YILSRAVKTLGKFDIVFCGRQAIDGDTAQVGPQMAEKLGIPQVTYLEQIVELAGRTIR
                     ARRNVGNGWEIVECRLPVLMTVLDTANQPRPAAAKRIMLYKRARAEAEIAADVAQQMP
                     QASADEQAREVSRRAADLKSRKLLIEQWDLEDIGADLAWCGMSGSPTKVHRIQSIVLK
                     KEGYAEVPATEEGIRKMIHELIVDHTLG"
     gene            13497..13721
                     /locus_tag="P5316_13080"
     CDS             13497..13721
                     /locus_tag="P5316_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRR85936.1"
                     /translation="MRARVAAVIEKIRPYIQGDGGDIELVDIDANNAVHVRLQGACSG
                     CPSAAMTLKMGVERAIKQQVPEITEVVRVG"
BASE COUNT         2958 a         4674 c         4363 g         2959 t
ORIGIN      
        1 taccatcgcg cggctgtatg ggcggttctc gctgtatgac ggtcgcggcg gctcaaatct
       61 cagcccggtc cggggaggag gccgagctga cattggcttc cctggacagc gccatccgca
      121 aagcccatcg agccggcgcc cacctgctgg ttctgcccga atgtgcatac ccggcttacc
      181 tgctcggttc ggttgaatcc taccgggccg gcaggtacat gcccggcgag gatttcgttc
      241 gatggctggc cgggcgggcg gcggattgcc ggatgcacat cgtcagcggc tttgtcgacg
      301 atggcggaaa ccggctgcac aatgccgccg tgttgctcga cgaccacggc cgcgagattg
      361 gccggacgca caagacattc ctctggcacc tcgaacatga ctggtacacg gcaggtgacc
      421 agattcgcac gttcgacacg cagctgggcc ggatcggcat gatcatctgt gccgagaccc
      481 gttcgcccga aatcctggcc acgctggccg ccgacggcgc cgagttgctg gccatgccga
      541 cctgctgggt taacagcgcc cgtcaggccg gcgaattcca caatccgcaa gtcgagtttc
      601 tgatcgaagc tcgagcgcgc gagtttggtt tgcccatcgt ctgtgccgac aagtggggcg
      661 tggaactgcc cgggttcggc tatgtggggc agagccggat cgtccgggca gacggttcct
      721 tggccgcaga ggccggtccg accggtgagg ccgtgatctg cgcgcaaatc accccggcga
      781 aatccaacag gcaggcggat ccaagggaac acacggcgag gctgctgagc cagtctgcac
      841 cggtccggtt gagggcttct gatctgctcc ccctccgggt ggctttgatg actgcatttg
      901 ttcggccgcg ggcacttgcc gccaacgggc cagtgctgcg ccacttgcag caacataagc
      961 ccgaccttct ggtggctcag gcggctaata tcgacgacga gaggaacctg cggcgggtcg
     1021 cggcagatct tggcgtccgc ctcctgctcc cgccacccgc atgccaaatg atcgatctgg
     1081 ccgctggtcg tgctgcctgc cttgtgggtc ccgcggcccg ttccttcgca acgccaagaa
     1141 gcatggtgct cgacggcgcg gccatgttga tcttctggga ggagccgtct gatctgccga
     1201 ttctacgggc gcgggccctc gaaaaccgcg tgttcgtgat cgcgatcaac acttccttcg
     1261 tcgcgatcat cggacccgat ggcacgattc tgacccatac ctcgtctgaa tccacgctac
     1321 cccctgtcgt tgttcttcac ttgggcgagg cggcagacaa gttggttgct cccaacaccg
     1381 atgttttctc cgagcgacga ccggcgacct accgattctg aaatctcgta ccgaacaggt
     1441 ttgcagacca agcaggcggt ggaaccagta taagacacga acggacggac attcgccagt
     1501 gaagggtttt gcggggccgt tccgacgatt cttattatat ttagggtgtt ttccctccta
     1561 ggcgcgcaaa cgtggccgac tcaaaatgct tgcgcttctt tcccctccgg aaggcgaagg
     1621 cgtctggtgc aaaccaaggc atcttcgcct tcttttttct ttctgacggt gccggaaacg
     1681 aacccgagac cgtctgtgcg gttctttcgg agcttcagcg cagactggga acccattcac
     1741 aaaccaaccg caccacaagt aggccttcag gcgtgaccgg tgggctatac cggacaggca
     1801 cggctggctt ggtaaaccgt ggctgcggtt cgcaagacgc tttcaaacag ccgtggaaga
     1861 agcgatcaca cggaaggttc gggcccgctt ctcggcgcgt tcggcaatca caaggacttc
     1921 ggaaactcct ggccggtgat gcgctcgtag gcctctatgt acttgcgtgc cgtacccaga
     1981 atgacgtcgg tcggcagtgc cggcccgggt ggtgtcttgt tccagttcag cgtctcaaga
     2041 taattgcgga caaactgctt gtcgaagctg ggctggtccc gaccgggctc atactggtcg
     2101 gcgggccaga atcgtgacga atccggcgtg agcacctcgt cgatcaggat cagctcctcg
     2161 ccgtcgaagc cccattcgaa cttcgtatcg gcaatgatga cgccgcgccg gcgagcatac
     2221 tccgccgcct gggtatagac agaaatgctc agccggcgca cggtttccat ggtcttgggg
     2281 tcagttaact ggcaggctcg ctcgaacgac acgttttcgt cgtgtccttc ttcggccttt
     2341 gtagcgggtg tgaaaagcgg ctccggcaat ttgtcacact gccgaagacc tgggggcagc
     2401 ttgatgccgc aaaccgattg cgttttctga tattccttcc acccgctacc ggccaggtaa
     2461 cctcggacca cgcactcgat gggcagcacc ttggtcttct tgaccagcat cgaacggccg
     2521 gcgagttgat ccaagtatgg ctcgaatccc ggcggcgcct tctcgtcgat gaactcgatc
     2581 aggtgatggc ggatgccttt gacaaagtcg aaccagaacc tgctgatccg cgccagcacc
     2641 tcacccttga aggggatccc attgggcatg acaacgtcga aagcgctgat gcgatcgctg
     2701 gcgacgatca gcaatttgtc tcccaggtcg tagatgtcac gaactttgcc ccgccgaacc
     2761 ggaaaccccg gaatgttcgt ctgcagaatg atacgctcgt tttccaaaac gcagctcctg
     2821 tattctgaga gtcctcacgc tgtccgactg cgaggcacag gcaacgcttg tggaccggag
     2881 aaagcaacaa tcgccggcca atgctccgag cctgcatcct cgcgccgagg ctccgtcgtg
     2941 agggcgcggg ctacggcgta ctgatatcaa ccggcacgag ctcgacattt ccgccggggc
     3001 aagtcgtcgg atacttgccg ggcggtggac catccttcag accctgccgg acgtactcct
     3061 cgatcagcaa ctctcgaggc actacctcct tccggtgaga ctcttcgagt cgcttgcgct
     3121 gggcaaggat ggcggctcgc tgcttctgcg tcaagttgcc cttggcctgc gccttcacgg
     3181 ccttcaaaag caaacccaac cggtctttct cctggatcaa ggccgtcctg aacaccgagt
     3241 ctgttcgggc cttcggatca aggcgaattc gttcggaaag caaactgatg gtgtactgaa
     3301 gatgcttggc catcgcctcg gcctcagccg gcgtgccgtc gtaatcctcg ggatgatcga
     3361 gccaatcaac ggccgtggtc gggcgctgga aaggccaagg gcagggccga gacgcagtcg
     3421 ccttgggcga accggacgat ggcgccaggg ttgaagtcgg gcgaagtgac gaaagaccgc
     3481 ctccaagaga accagagggg cgttttgcag acgagttgga gttcgtcgcc gggctgccag
     3541 ccggtgcagg ttcgttgggc tctcgttcag ttgcagccga tggcaagtct gcctgaggcg
     3601 ggactggccg aacggcggtg tcactggcgt cctcgggcct gcggtcgacg gtcatgtatc
     3661 gagggtcatc ggtttgagtc tttagctgcc cgggtggaag ggcttgcgga gcgacggatt
     3721 tgaactcatt cttgccacca ccgatagacc ggccaagggc ctcctcatga gcgcgggtca
     3781 gcagcttgag caaagcaggc ctttcgtagt gggtcacata aggcagacgc atgcgctcgc
     3841 ccggcttagc gggctcggcg gacttgaagt gactatcggc ggccagttcg gccagcgaca
     3901 tcttcaaccg cttctgtacc tcggcggaca tgtaggtcaa ttctcccggc gacgacttgg
     3961 cgatgagctg cgtgaccagg aaacctgccc attcgaagtt cctgtcctga tacttggaga
     4021 tgaccagatc gagctgctcg agcttctcca cgtcaggctg aatcggcgga gcctcaatct
     4081 cggccggagc agatgacggt tgcgattggc tcgggccgcc tcgctcgacg cgggcaatgt
     4141 tgaaccgatg gatctggcga acaaaggcga tgccgtccgg ccggctctgc cgcaaccaaa
     4201 cctcatcttc ggtctcgttg acgatctgcc cttcgagccg ctggccgttg tacatgatca
     4261 cagcgtcggc ccgtgtcgag acggccgtcg acagcactag cagaccggtc atcagaaacc
     4321 gacgtgtgtt catcgcccga catcccagac gcaaatcgtt ctttcgcttg gaccgcgccc
     4381 gcaaccgcga ccctggaggc acgccctctc caatctgctt ttcaggcggc caagcattat
     4441 acgccgcgcc caccacccgg gaacagaatg ggcggaacga acctggctcc ggacgcatgg
     4501 gaggtcaggc ggcgtctatt ccggataagg caagcagatt caacgttggt cagaggcgat
     4561 gggtaaaggg gcgcagcgga ctcgggatgc gggcggaggt actggcttgc gaacagggac
     4621 ctcatccgga aaccgcgccc aatccggcgg cgtcttcatg agtttcatcc acctctgccg
     4681 gctcaacgac ttcgatccct tcgatttcct gaccgaactg caacgtcacg ctgcaacccc
     4741 ggccgctcga cctgagaact ggatgccttg gaaccgccgc gaaggcctcg gggctacctc
     4801 tcctgcctga ccggctgcga ccccaaacca cgctgcgcac gcttaccgaa aggacacgaa
     4861 tcggacggga acggggatgt ccagcgggac tgcccgttac tctcgcatct tcgggtactt
     4921 ggtctgtcgc tgccaggccg gtcgtgcgtt gcgaatctca acgaccaccc aacccacgca
     4981 gatcagaaag accatgaagc acgccatact gaacaatagg atcaggctgc tcaagccgct
     5041 ggccgccgcc tccggccggg atcgccgtaa cggacggtcg gggctggccg gtcccggttg
     5101 agtggccggc gccggtgcgc tggccgtcac agccgccggc tgactcgtca gcggcttgtg
     5161 tttgatgtac cgactcaaga gcagcaagac cacaccaccg accaaggcca gcaatccgta
     5221 caggattggt ttccagggca aaacgaatga ggatttgtgc tttgacatgc gtttggtttc
     5281 ccagattgag cactcgcccg tcccgtagcg gcaagccggc actcttctgc cggatcatcg
     5341 atcgcgactc acgacaattc atacaccatc gcaacctcgt cacaatgctc ctggagggga
     5401 cagcgaatgc agtccgtcca taccttgtgc ggcaaagtct ccttgggaac aacagcaaac
     5461 cccaacttct cgaagaagac cttctcgcgg gttagggcaa aaagccgctt cagcgccagc
     5521 cgtcggcctt cctcgatggc ggcacctacc aggtgacgac ctattccccg accacggtac
     5581 gattcctcga cagccagact cttcacctcg gccaaatcgc gccacacgag ctccaccgcc
     5641 acacaacccc ggacttgctc gccctcgctc ccgcattcta cccagacgag aaagtcgcgc
     5701 agatgctcgt aaagatccgc atacgaacga aacagcatcc gattcagctc agcgtgatag
     5761 gtgaccagcc gatggatttc cggcacgtct ttcatcattg cgttgcggat caatgacggc
     5821 tccgtaaaag cggccagcga ggacaaaaca atcgaagtgt atcgtaagca ccccgaaatc
     5881 agaaacaagc gcgtcaggcc cgggtcattg aagcactcac aagcagccgg gagcaagccg
     5941 caggggtttg ttggaccggc aaggcacgct gcggcgcttg tcctgcaagg cgacttgctc
     6001 ccatctgcgc ggctacatac cattgttcct tatcggacgc aggaggcaga cggccaagcc
     6061 gtcgtccggg caattggaga cctgcttggt cgctcccgca cagatcgggc ctgtctctgc
     6121 ttgccgcgtt gcatcgtccg atccgcacga cgttctaacg aggccgctgt tgagccatgg
     6181 acttgttttc tcaacagaga aaggacgctt tcaaccgggt ccagccgttg gccgtttcaa
     6241 tgcgcccccg cacactggaa gagttcgtcg gtcaggaaca ctttctcggc ccggggaagc
     6301 tcctgcgacg cctcctggcg gcggaccgcc ttagcagcgc cattttctac ggcccaccgg
     6361 gtaccgggaa aaccacgctg gcccatatca tcgccggaaa aacccaagct gcgttcatcg
     6421 aagtgaacgc cgcggcggtt ggcgtgaagg aggtccgggg gatcctggaa gaggccaaag
     6481 aacggcttgc cagcactggc aagcgtactg tcctgtttct ggatgagatc caccggttca
     6541 accgtgctca gcaggatatc ttgcttaacg acgtcgagaa cggtctcatc atcctgatcg
     6601 gagcaacaac ggaaaaccca ttcttcaccg tcaactcgcc cttggtatct cgcagccaga
     6661 tctttcagtt cgagcccctg accaaagagc atatccgcat tctgctgcgt cgtgcggtgg
     6721 ccgatccgga gcggggcctt ggccgataca aggtcgagat ccaacaggag gccattgagc
     6781 actgggcgac gacctgtgac ggagacgcac gcagagcgct actcgcgatg gaggtcgctg
     6841 ttctgtcaca aattgattca acaacatctg aagcgtcgaa cccaatcgtc gttgacctca
     6901 aagtagctga ggagtcgatc cagcggaagg cgatcactta cgatggaagc ggagatgagc
     6961 attatgacgc tgccagtgcg ctcatcaaga gcatgagggg cagcgatccc gatgcagccg
     7021 tctactggtt ggcccgcatg ctggaagcgg gcgaagatcc ccgattcatc gctcgtcgag
     7081 tagccatttg tgccgcggaa gacgtgggca acgccgaccc ccatgccctc gtggtagcca
     7141 atgccgcagc ccaagtcacc cttttggtcg gcatgcccga atgtcaactg gtgctcagcc
     7201 aagccgtgat ctatatcgcc tgtgccccaa aatcgaacgc ctcagccgca gccatctgga
     7261 cggccaccaa ggatgttcgg gaggcccgga ccattcctgt cccaaagcac ctccgcgatt
     7321 cacattatcg gggagcggcc agaatgggac acggaacggg ctataagtac gcccacgact
     7381 accagggcgg ttttgtggag caagattatc tcggcgtcga taaaacctat tacaccccga
     7441 ccgaccgggg gcacgaagcc gtgatacgcg cttacttggc ttcgctggct aaccagacgg
     7501 aaaaacgtcc aggcgggccg cttggtggtt ccaaagaaaa ccgaccccgc gagatcggaa
     7561 ctgacgattc aagatgaagc cggcaactac gcccgatcac gacgcctcaa agaaagaact
     7621 caggcaacgg ctgcggcagg tcctggacgg tatttcgtct gaagagttga gtcaccgctc
     7681 tcatttggcg tgcaataagc tcatcgccac ctcggtctac gagcagtctg atgtcatcat
     7741 ggttttcctg tcactgcctc gcgaggtgga tacgacacct ctggtgctac atgcttggcg
     7801 ggatggcaaa cgggttctgg ctccaaaggt gaactgggat caacgacgga tgttaccgat
     7861 tgagattgtc tctttgaccg acaatatcat tgggggggcg atgggtctgc gcgaaccggt
     7921 cgagggcacg ccctttcccg tcgagcagat cgatctggtg gtcgtgccgg gcctcggttt
     7981 cgacctcaaa ggaaacagga tcggacgggg gagaggtttt tacgaccggt tcctgaagca
     8041 cgagggctgg aaaggtacct cttgcggctt ggctcttgaa gaccaggtgg tggaacacgt
     8101 gccgatgagc gagcacgatg caacggtcga tatgttggtg accgacgcgg ccgtccgggt
     8161 ttttcggaag taggttgtgc cggtcatggg tcgtgtgaac tgctgccgat ccgcactagc
     8221 ggtcaggagt atcagaagtg gcttacggac gaaggcgccg tcgcaagtct cgtttcatgt
     8281 cccggttcat cattctgctg ctgatcgccg gcggcggcta ctacgcgtat gtcaagtact
     8341 acaagaaacc gggagcccca ggcccggagt ccacggcgac ggctccgtcc gacgtccgaa
     8401 cacaacctcc aggcggtggg gtgctgactc cgcctctgga ggagcagttt ggcgggatcg
     8461 ccggatcgca acccgcctcg caaccgaccg ccagtcgtcc cttctgggca acggttcagc
     8521 cttttgagaa ggagcctatc gcagtaccgc cggccgatca gattgaggcc gtctatcagg
     8581 agggcatgca ggcttggcaa gccggcgacc tgttgaaggc ccgcgatgcc ctgaaccgga
     8641 cactgcacgg cgacatcccg gtcgagcggc aggcgcagat tcgcgaaatc ctcaatcaga
     8701 tcgctgagaa gaccatcttc tcgctcgatc tcctcccggg cgaaacactt gccgaggact
     8761 acgtcgttca gcagggcgac atgatgcaga agatcggacg gcagtacaag attaccgagg
     8821 aactgatcag cagcatcaac ggcggcatga acaagaactt catccgcgcc ggccgccgac
     8881 tcaaggtgat caaaggcccc ttccacgcca ccgtggttaa gagcagtttc gagatgcacc
     8941 tctatctaca aaacgtctac gtacgcacct acaaggtggc attgggggcc gataacagca
     9001 cgccctccgg caagtgggtg gtcaccgaac gtctgacgaa tcccacctgg accaatccgc
     9061 gaaccggcca gcggatcgcg gccgatgatc cccaaaaccc gctcggcgaa tactggatcg
     9121 cgcttgaggg cattgaaggc cgcgccatgg gccagagcgg ctacggcatc cacgggacca
     9181 tcgatgacag cagcattggc actcaggcat ccatgggttg cgtccggctg tccagcaagg
     9241 acatcgaagt ggtctactca atgttggtgc ccggctcgac ggtgaccatc accgagtagc
     9301 cggtgtcgac gctcacagtg cccggggcgt tgagtttccg ccaagccctt cggccatcgc
     9361 tcactttggg acgggtaccg gaccggcgag agtctgatat tcgtccaggg cggatcgatc
     9421 gccgctgcag accagctcaa tgtcgcggcg gatggaaggc aggcttgtca tgatgaaacg
     9481 cctggcaacg cgccgtttcc gctcattgtt ttccgcctgg cgcagcagca ggtgaccgca
     9541 caatactgcg acggcactgt ccaccagcct gcgaccggac agatccatat actcgttgga
     9601 ttgcgttttg acgaaggcca cgcccttcag taccaattcc cgggcttcca gcagccgggc
     9661 tttcagctcg ttgagcaggg agtcggagta ttgacgggtt tcgtattcgg cggcctgctt
     9721 ctcgaatact cctgagcaca cgccgcggac ggccgccacg atctgaagct ggcttgtgcc
     9781 ctcgtaaatc gtggtgatgc gggcatcgcg gagataccgc tccacaggat agtccttcat
     9841 gtagcctgag ccgccgagca cctgcagcgc gtcattggcc accttgatgc acatctccga
     9901 cgcgtaatac ttgctcatgg gagtgagcat gccgttgagc cgcttgtagg tccgggcatc
     9961 cgcctttcgc cgcttgagct cctcgggatc agtgggcgtg aagtagtcca ggatccggat
    10021 gttgttgttc tcgaagtcgc acacgcggca ggtctcgtag accagcgccc gggcggcttc
    10081 gatgtgcacc ttcatatccg tgaccatctc cgccaccgcc ggcagttgct cgatcggcac
    10141 gccgaactgc ttgcggctgt gagcgtattc gcgggccact cgatacgcgg cctcggcgat
    10201 gcccagcgat tgccccgcga tgccgacccg cgcaccgttc atgaggctca tgacgtaggt
    10261 gatcaacccc cgttgtcgct cgccgatcag ccgggcaggt acgttctgaa aaaccagctc
    10321 gcaggtcggc gagccgtgaa tgcccagctt ctcttccagg tgacgaacgc gaatttgcgg
    10381 tccgcgctcg gcaagaaaca ggctcaatcc gcggccgtcg gagatctccg gctcgctgcg
    10441 ggccagcgtc agcagaacct cgccgcagcc gttggtgatg aaccgcttca cgccgtccag
    10501 ccgccactgg ccgtcctcat cctggtacgc gcgcagactc accgattgca ggtcgctgcc
    10561 ggcatcgggc tcggtcaata ccatcgctcc ggtcacttcg cctttggcaa accgcggcag
    10621 gtattcagag cgaatgtcct cgccggcaaa ggcataaatc gtctcggcga tgccttgaag
    10681 gccgaaaatg ttcatcagcg aggcgtcggc ccggctgacg atctcgatcg ccatggtata
    10741 gatcaaacag gggcaattca gcccgccgta tcgccgcggg agcgtgaaac ccatcagatc
    10801 ggccttggcc aggacgttga ggttctcgcg cacgttctca ttcagcgtca cggtgccatc
    10861 gggattcagc cggtttccct catggtcgat cttttccgcc cgcggcgcga tatgatcgcc
    10921 ggcgatttcg ccgaccaggg tcagcacgcg acggtagttg tcgatggcgt cggccgcgtc
    10981 caccggcgca tattcgtagc cgttcccgcg gtcgcgggtg aagttatcct cctggacggc
    11041 agcgatctcc gccaggtcga gatggttgaa caaaaactga aggtcttcat tgtcgagaaa
    11101 gaaattcgcc atgtttaccg ggctcgttcc ttgatcgcct tgatcatcat cggaatcact
    11161 ttgttcagat cgcccacgat gccatagtgt gcgacggaga agatcggggc gttggcgtcc
    11221 ttgttgatcg cgatgatctt ggcggactct tccatgcccg cgcggtgttg gatggccccg
    11281 ctgatcccgc aggccacgta cagggccggg cgcaccgtgg tgccggtctg gccgatctgg
    11341 tgttctttgc cgatcaggcc gctgtcgacg gcagcgcggg aggctcccac tgcggcgccc
    11401 aggacaccgg ccaaatcgta gatcaactgg aaattctcct tgctgccgac tcccccgccg
    11461 ccggccacga tcacgcgcgc acccttgagg ttaaccttct gcttctcgcg gtgtcgttcg
    11521 acgattttga ccaccccggt aacgccatca aggctgacct tctcgagaat gaggtccccc
    11581 gttcgtttcg ggttgccatc ggccaggcgc atgacgcctt cgcgaacggt cgccatctgc
    11641 ggccatcgcc ggggattcac gatcgtggcg atgatgttcc caccaaaggc cggccgaatc
    11701 tgcaagagaa ggtcctggaa cgtctccttg ctcttgggat cggtgtagtc gccgatgtcc
    11761 aagtcggtac agtccgcggt cagaccggcc ttcatttccg aggccacccg gggcgccagg
    11821 tcgcgtccca gcgccgtggc cccatacagc acgatctgcg gcttatgctt gttgatcaac
    11881 tcacagatga cgcgggcata cggtagcgtg cggtagtact ccagttgcgg atcgtcaacg
    11941 caatacacgt tgtcagcgcc atgggcgatc aaccggtttg acaagtcgcg cactcgatac
    12001 cccggcaaca ccgctcccat cttgaccttc aggcggtctg ccatctcgcg ggccttgccg
    12061 cagagctcca gcgatacctc gctgagattg ccgtcttcct gctccgcgaa gacccagact
    12121 tcacctttcc gattcggttc aatcatatct gctcgactct cagtccaagc cgtcaaaccc
    12181 cggttttccc ccggaaactg caaccaccgg aacgggacgc cgacggccga ccgctgatcg
    12241 cttacgtcac cccagtgtat gatccacaat cagctcgtga atcatttttc ggataccctc
    12301 ctccgtcgcg ggaacctcgg cgtatccttc tttcttcaac acaatcgact ggattcggtg
    12361 gacctttgtc ggcgaacccg acatgccgca ccaggccaga tccgctccaa tgtcctccag
    12421 atcccactgc tcgatcagca gcttgcggct cttgagatca gccgctctgc gggagacctc
    12481 cctcgcctgc tcatcggccg acgcctgcgg catctgctgg gcgacgtccg cggcaatctc
    12541 ggcctccgct cgggctcgtt tgtacagcat gatccgtttg gccgccgccg gccgcggctg
    12601 gttggccgta tcaagcaccg tcatcaacac cggcagccgg cattccacaa tctcccagcc
    12661 gttgccgaca ttgcggcgtg cccggatcgt ccgccctgcc aactccacga tctgttccag
    12721 gtacgtaacc tgcggaatgc ccaatttttc agccatctgt ggaccgactt gggcggtatc
    12781 gccatcaatg gcctgacgac cacagaagac aatgtcgaac ttgccgaggg ttttgacggc
    12841 tcgtgacagg atgtagctgg tcgcgagtgt gtcgctggcc gcggcacgac gatccgtcac
    12901 cagaatggcg cggtccgccc ctcggtacag gcagtcccgc agcacctcac aagcggtcgg
    12961 cggccccatc gtaaccgcag tcaccgtacc cccgtgcgtc tcgcggaccc ccagagccgc
    13021 ttcgagagca tgcagatcct ccggattgaa gatggcaggc aaggccgcac gattcaccgt
    13081 tccatcggcc ttcatggcct cgccggtgat gttggcagta tccgggacct gcttgacaca
    13141 tacaacgcaa tcgtagctca acccactgct cccacgctca cgacccgatc cgcccaaacc
    13201 accacccccg ccgggtgcag gcacacccct tccctggctc cgatcaggtg tagtcggatc
    13261 ttacccccga tgcagcaccg ggccgcctca ggcaatagag ccgagatgtt agccgtcgat
    13321 tcccagaccg tcaacgccgt ccctgcgtac cgcaagcccc ggacaagccc tcacacagtt
    13381 cgcttgtcct gaagcctttg gggaactata cttagggcag tcgtaatact gcgaccgtgg
    13441 ttactcacaa gaggcccaac accatgccag acacaaccgg cgatgcagca aactcaatcc
    13501 gtgcccgcgt ggcggccgtc atcgagaaaa tccgaccgta tatccaaggg gacggtggcg
    13561 atatcgagct ggtcgacatc gacgccaaca acgccgtcca cgtccggctt cagggcgcct
    13621 gctcaggatg cccctcggct gcgatgacac tcaagatggg tgtggagcgg gccatcaagc
    13681 agcaagtgcc tgaaatcacg gaagttgtcc gggtcgggta gtaccgacct gcccttccag
    13741 cggcaacgcg attagcatct gatccgactg ccgaatactg ccatgtcacg ggcgaaccgg
    13801 ccgcgcagcc gagtctcccc cgtcggccat ccgatggacg atgactcgtt gaaggccgtg
    13861 cagcagtcag tcatgcgcag cgcgcttctc cggaccgacg gctgtcaggc aagaaccgcc
    13921 gcccgaaccg ggaatctgcc tgccttcccg caggcgcccg cggaaacaac gtgatgaaac
    13981 agaccaatca ccccttcttg gcccaggagc aatcagcgcg ggtgcccgac ctcgtttttg
    14041 aggcggggga gcgacgatca cggcgggcaa cgcttccagc gaacaccccc cggcaagctc
    14101 gaacctcgcc agggtgcagg cggaatcgac ccgaccactc gcttctcggc aaacccggca
    14161 gagatcaagg catgcgcaat cgaccgccca gggaatcggc gatgattcat tgatgacgcg
    14221 cgggtcatcc attaagaact cttcctgtca ccccctctcc ttctggctga tgctgcacca
    14281 aaaagaagta tgaaatccag caaatctcaa aaaaatggtg ctcaaccctt gccaaccgat
    14341 gatctctcgt gtatgcttct tgcagttgta tcaaattgca ggatctcaga gatgaagtag
    14401 ggtaacgcat aatgcggtct acccttcttc ttcttcttct tgttgttgtt gttgttgttg
    14461 tcgtctcagg cagtcggccg gttcatcggc tccggtgaac acgaccaaag gaggtctgcg
    14521 cacatgaaac ggctcatcgt cttggcggtt cacacaggca acgaaggacg gtttggcatg
    14581 tatccttgtc cggtatccag gagcgggagg actctatgag gaattctaga tgggcccttg
    14641 ctttctttcc gtcgctgact gttattgcca gcacggtcgc aggcaccggg actgatcttc
    14701 cggaggacaa accaatccac gagatggcgg acagtgaatt gcttacgtac atgggatggc
    14761 tccgcaacac agttactgag ccgtcggagc gacttgcgcg ggtggccata cgttctgtcg
    14821 ggcaaccata ttgctttggt tcatcctcgg ccatgttcga tcacaggaaa agcgattgcg
    14881 tcacctttgt cgaacactcg atgccagcgt cgttctgaaa gtgcagagat ttcgccggtt
    14941 tgaatatgta gtct
//
LOCUS       DAOTPW010000137        14932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3930_length_14932_cov_5.632318, whole genome shotgun sequence.
ACCESSION   DAOTPW010000137 DAOTPW010000000
VERSION     DAOTPW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14932
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3930_length_14932_cov_5.632318"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="P5316_13085"
     CDS             <1..809
                     /locus_tag="P5316_13085"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85937.1"
                     /translation="GNLGKVHLIHVLNMKMRESIGHKADAPTPPGVDYDQWLGPAPKR
                     DFNPNRFHYSWHWFWDYSGGDIINDGVHQIDIARWLAGQDSPAAVSGTGGRFSFNDDQ
                     ETPDTQVVTWDFDNLTMVFENILWAPYMKKMEWNTRDTDAYPNWPFDGMRIEVYGEKG
                     LMFFERHGGGWQVFDESEKIISQSNDRHPHYAHLDNFFECIRSRKRPNADIEEGHLST
                     ILCQIGNISYRCDGRRLKWDGKQEKFTNDEEANGYLKRTYREPYVVPETV"
     gene            828..3182
                     /locus_tag="P5316_13090"
     CDS             828..3182
                     /locus_tag="P5316_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRR85938.1"
                     /translation="MHALGNRSNSRKWGAISCIGIGLLLAVEAVGTAHADEGRLPNAS
                     PLVETGDLAMKMVAGIDKHLLRQTEASVADRERYWHRDFGSTEAYLKSVEPNRAEFAR
                     IVGVVDPRREPAGLQLIATTTQPSGAGGSADYTIDRARWPVFDGVDAEGLLLQPKGNP
                     VARVIALPDADWTPEMLVGLAPGVDPACQYARRLAENGCLVLVPTLVDRGSRLSGNPS
                     IRMTNIPHREFIYRMAYQMGRHIIGYEVQKVLAAVDCFHSGAAGRKLPIGVFGYGEGG
                     LLALYAAAIDRRIDAACVSGYFQSRAQVWAEPVYRNVWNLLREFGDAEIASLVAPRPL
                     VIEAGRGPEVGSPPPDPYGHSQASSGRLVTPPREAVLHEFNRAQGVYEQLKAAGKIRL
                     VFPDDPRAGPGTEPALQAFLAALVGRQGPLRPAGPSPVPSQWADADVDARQARQFGQL
                     VGFTQRLVRESGERRRQFWSKAGASSLEKWQKSCEYYRRYLWDEVIGRCPAPGDSINP
                     RSRRIIDNDKWAGYEVVLDVYPDVFAYGILLLPKDIRPGERRPVVVCQHGLEGRPNKV
                     VDRTIKSPYKAFGADLADRGYIVFAPQNPYIGGDAFRLLQRKANPLKLSLFSFITAQH
                     RQILLWLGQQPFVDPKRIAFYGLSYGGKTAMRVPALLPEYCLSICSGDFNEWIVKTTS
                     VDLVNSYMFTYEYEIYEFDMGNTFNYGELAGLIAPRPFMVERGHDDPVGMDEWVSYEY
                     AKVRRLYAKLGLPDLTEIEYFDGRHEIHAVGTFAFLHKHLARPR"
     gene            complement(3324..3656)
                     /locus_tag="P5316_13095"
     CDS             complement(3324..3656)
                     /locus_tag="P5316_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85939.1"
                     /translation="MDSKPQTLTATVEGRFGIERTVVLVRQPDGHYVEANETRELPTD
                     SVEGGSCGNQQARSSKPSEQPNEHDTHQRLTAKVQGQFGEDHEVVLVRQPDGKYRELA
                     DPAQALVL"
     gene            complement(3999..5489)
                     /gene="pyk"
                     /locus_tag="P5316_13100"
     CDS             complement(3999..5489)
                     /gene="pyk"
                     /locus_tag="P5316_13100"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRR85940.1"
                     /translation="MSHSNKTKVVATVGPACRDQATLRALIDEGVDVFRVNLSHGNLE
                     EHTQTVAAIRRAAVQSGDVVAIMGDLCGPKIRVGQIADGSFDIAVRELLAIEPGSFPC
                     TPGRICTSYDQLCDDVQCGERVLIDDGQIRLRAVAKQGAKLICECEVGGTIRTHKGVN
                     LPDSRLSLPALTQKDRTDIQWAAQQDLDYLALSFVRSPKDLEELRAELTRHESNAHVV
                     SKIERPEAIEHLDEIISLSDALLVARGDLGVEMDVSRVPLLQKQITQRCARAGKPVIV
                     ATQMLQSMVESPVPTRAEVSDVANAILDGADAVMLSAETSVGGYPLEAVRVIRSIADQ
                     TEAYLAGQAGDSVPRVDPEELQFVSAVVHGASVLARELNVHALAVWTDTGTTVRLLSK
                     RRIPQMIVGLSPDARVCRRMSLYYGVRPARLDHQTRQKDMIRDVDAVLIDRELAKTND
                     MIMIVAGTHLREPGSTNAMLIHLVGACDACSPSATREAVRVPVMTS"
     gene            5881..7476
                     /locus_tag="P5316_13105"
     CDS             5881..7476
                     /locus_tag="P5316_13105"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRR85941.1"
                     /translation="MRFNRSCLPILWLLVPLCLDWPPASAGQEPLNRPNILFLMADQF
                     RADCVGADGNPVIRTPNLDRIAAEGARFRVAYTSTPSCTPARSGILTGLSPWHHGMLG
                     MGRMALSYQNEMPRMLHEAGYYAAGIGKQHFHPQRNAHGYDLLILDESGRATSPDFRS
                     DYRSWFLSMAPTLNPDATGIGWNDYRAAEYALPESLHPTRWTGDVAVNFLKEYRRPEP
                     FFLMVSFARPHSPYDPPGRFMKMYEDADVPPAVVGDWVSRHAGKDNPEDFNSWRGNFG
                     PEQVRASRRAYYGSVTFIDEQIGRILAILQERGWLDNTLILFTADHGDMLGDHHLWRK
                     TYAYEASARIPMLIRWPSSINAARGQVLAQPVELRDLLPTFLDAAGATVDEKRFDGRS
                     LLRLVRGRSDGWREYIDFEHSACYAGAGYWTALTDGRTKYIVHAAEGQEQLFDLQADP
                     GELHDLAADPAQQDRLRAWRERMVTHLSERGEPFVKEGKLVLPRPSMLYSPNFPKDEG
                     EENSAVGKARQSAQTRPTGRPRR"
     gene            7499..9628
                     /locus_tag="P5316_13110"
     CDS             7499..9628
                     /locus_tag="P5316_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HRR85942.1"
                     /translation="MSVSTGSAIGVLCGLVSILFSPDVQAVSASTPARPRVLIIGDSI
                     SIGYNEPVREMLSDVADVAHNPGNAADTWKGLEELDTWLGSEKWSVIHFNWGLHDLKH
                     VRDGKLDLTAPRVSTTEQYRENLDKLVLRLKESGAKLIWASTTPIPEGAAGRIKGQEI
                     EFNTAAREVMDRHGVTVNDLHTWVWPHLGRYQQKANVHFTEEGSRFLAERVARKIRNT
                     LQDQRPPFEMPAVQPPVFPDRHVSIEQFGAVGDGKTLNTSAIARAIADCAEKGGGRVL
                     IPPGVWLTGAIHLKSNVELHLAEGAELRFSTNPADYLPAVFVRWGGFECWNYSPLIYA
                     RDCVNIAVTGRGKLEGQGKAWWGWVKEQDRVSRRLYDMVLAGVDVEQRLFADEKEPLR
                     PQFIQPINCRNVLIEGVTITSGPFWTIQAVYCENVLVRNITVTNEGPNNDGFNADSCR
                     NVLVEHCTFATGDDSVAIKSGLNEDGWRVGRPSENIVVRHCRMMRGHGGVVIGSDMSG
                     GVRNVFVHDCDFSGSQIGIRLKSSRGRGGIVENVYYRDIDLGDIQGQAITIHTDYKAY
                     FGADQGKAPAFRKIHISDVICRKAAQAVRLSGLPEQLIEDVVFERAILGAKEGMTCSQ
                     VKGLRLADVAVTPAAGPVMRVKDSRQVTLQRAVCPRDADPFLRVEGAGSGEIRVMESD
                     LSLAKKSVELAEGAPADAVAIDDKPVR"
     gene            complement(9980..12190)
                     /locus_tag="P5316_13115"
     CDS             complement(9980..12190)
                     /locus_tag="P5316_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85943.1"
                     /translation="MTHLIRYLAILFLFVSAVIGVGRALAGEPVRATEQTTGPWQLFI
                     DDYLIVSKKNVVRRYHPFRKHPDNPLIRVDRPWEAHVVNAVTVLPGEDGTGFRMWYYC
                     WTEDKSSKKGRGSFMCYAVSKDGLKWEKPNLGLYEWSGDGTKNNNIVPHCPGHVMYTP
                     WEKDPQKRYQGVGGMYHAFSSPDGLNWKQESTETIISGGDTSHFYWDAHTKRFRCTVK
                     GGANAYVSGDVSGLRRRVVGFSETTDLTKFPPLRMVMAPDDVDDLWCKPETVQRTHFY
                     ACPVLPYETMYIGLLQIYRAEEPEGYFHGPLWLELVSSRDGVHWLREEADTSVRNIYA
                     LDSVSRPPLLDIGKYREFDRGMVIAHTPLLVGDELWTFYTGYDEEHDLLPYRSAIGLA
                     KLRKDGFASLDADELPGEVLTKRFENAEGPLLVNYQARGGSIRVELIGDDGLVVAGYG
                     REDCEPLTGDAIRGVVSWKTRKELPNGVPVRFRFILDRGRLYSFMAGQHAKSIDEPPV
                     PVLQALYTFEGDTEAWSDMLGEDGLQALRNLGTSTLDHKNPDPAFGRRSLVVGSQWRP
                     WNRVEILGTDDLGRYFTLAAMVKSSNNKHARLFSAYNGNFPVSSAELIFDFDPSGRML
                     NGLRLVCKGIAVDSEAVTFNDGKYHHLAVVYDDGCVTFYLDGKPIGRQWIPGGEPVKL
                     ARSLLIGEDAHRGSDEQLMGNVDDVLVLGRAMSAEEIASLAKNGATVFFAKTDK"
     gene            12834..14168
                     /locus_tag="P5316_13120"
     CDS             12834..14168
                     /locus_tag="P5316_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85944.1"
                     /translation="MLGITRRTGSVLVGILCLGLVPIAIGQQAEQNGDQGSRRRGPRG
                     GRQDNQQTAQQGEQQDGQRGGRRGSRREGQQGFQEGGPRDRQSGPRQEGEQGPGRPDR
                     RGPRTFTPEDRERFYNEMVNRYMERLSDTYELSDDQQTQVRSRLEELRSQQRTYSEQH
                     QKEFDDIRTQMEQLRESGAGFGSPQARELFQKMETLREQSPLMNRDTVTAEVEKLLPP
                     EQVEKGRARREAERQEWDRRREEMRRRWEERRQQEGDQSQSDDDGPMRFFRDRGDRRR
                     RDRGEEGQGQPVEGQENQEQQGPEGQDQQQGQWPQIDGRPVVQWPVEDPLGPWDRYVR
                     DFARRYRLDESQRATAQSILREMHERRQVYEQAHRQDYEAAHSLQGQEREKRLAQLNQ
                     PIEFLFSQLKSRLERIPTTEQRRAAGELRTTSRPATPPSSAPAGATSQPAGG"
     gene            complement(14296..>14932)
                     /locus_tag="P5316_13125"
     CDS             complement(14296..>14932)
                     /locus_tag="P5316_13125"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRR85945.1"
                     /translation="YHELADATGDPSVKAASLRHHRLVMETFLRPARGLILEWLDMAG
                     NEIAPPEGTVVLPGHAIESMWMQLHIALETGESTIIERAVEAVRRHLEIGWDAEYGGL
                     FLAVDADGRSDVAWRFPEAKLWWPHTEALYATLLAYEISRQDWCLEWYQRLHDYSFSH
                     FPVKPHGEWTQKLDRRGHPITDVVALPVKDPFHLPRALIYGMETLNRLSSA"
BASE COUNT         2896 a         4599 c         4587 g         2850 t
ORIGIN      
        1 ccggcaatct cggcaaggtg cacctcatcc acgttttgaa catgaagatg cgggagagca
       61 tcgggcacaa ggccgacgcc ccaaccccgc cgggcgtgga ttacgaccaa tggctcggac
      121 cggcgcccaa gcgggacttc aaccccaacc gcttccacta ttcctggcac tggttctggg
      181 attactccgg tggcgacatc atcaacgacg gcgtccacca gatcgacatc gctcgctggc
      241 tggccggcca ggattcgcct gcggccgtca gcggtaccgg cggccggttc tccttcaacg
      301 acgaccagga gacgcccgat acgcaagtgg tcacatggga tttcgacaat ctgacgatgg
      361 tcttcgagaa catcctctgg gccccgtaca tgaaaaagat ggaatggaac acgcgggaca
      421 ccgatgcgta ccccaactgg cccttcgacg ggatgcgcat tgaggtctac ggcgagaagg
      481 gcctcatgtt cttcgagcgc cacggtggcg gctggcaggt cttcgacgag tccgagaaga
      541 tcatcagcca gtccaacgac cgtcatccgc actacgctca tctggacaac ttcttcgagt
      601 gcatccgatc tcgcaagcgg ccgaatgccg acatcgagga aggtcatctc tccacgattc
      661 tttgccagat cggcaacatt tcatatcgat gcgacggccg caggctgaaa tgggacggca
      721 agcaggagaa gttcaccaac gatgaggagg cgaacggcta tctgaaacga acgtatcgcg
      781 aaccctacgt ggtgccggag acggtctgac cggtggagtg gccttgaatg catgctcttg
      841 ggaaccgcag caactcccgc aagtggggag cgatatcgtg cattggaatc ggccttctgc
      901 tggcggtcga agccgttggc actgctcacg cggacgaggg ccgcttgccg aacgcttcgc
      961 cgctggttga aaccggcgac ctggcgatga agatggttgc tggcatcgac aagcatttgc
     1021 tgaggcagac tgaagcatcc gttgccgacc gcgagcggta ttggcatcgc gattttggct
     1081 cgaccgaggc ctacctcaag tcggtcgagc cgaaccgagc cgagttcgcc agaatcgtcg
     1141 gcgtcgtgga tcctcggcgg gagcccgcgg gcctccaatt gatcgcgacg accactcagc
     1201 catccggcgc gggtgggagt gcagactaca cgattgaccg cgcacgctgg ccggtgttcg
     1261 acggcgtcga tgccgaaggc ctcctcctgc aaccgaaagg caatccggtt gcccgcgtca
     1321 tcgccttgcc ggatgccgac tggacgcccg agatgctcgt aggccttgcc ccgggcgttg
     1381 atcccgcttg ccagtatgcc cggcgcttgg ccgaaaatgg gtgcctggtg cttgtcccca
     1441 cgcttgtcga tcgaggcagt cggctgtcgg gcaatccttc aatccgcatg accaatattc
     1501 cccatcgcga gttcatctac cggatggcct accagatggg tcggcacatc atcggatacg
     1561 aggtccagaa ggtcctcgcg gccgtggact gcttccacag cggtgcggcc ggcagaaagc
     1621 tgcccatcgg cgtcttcggc tatggcgaag gcgggttgct cgcgttatat gccgcggcga
     1681 tcgaccggcg aatcgatgcc gcctgcgtca gcggctactt ccaatctcgc gcgcaggtgt
     1741 gggctgaacc ggtctatcgc aacgtgtgga acctgctccg cgagttcggc gatgccgaga
     1801 tcgcaagcct tgtcgctccc cggccgttgg tcatcgaggc cggccgaggt cccgaagtgg
     1861 gcagtccccc acccgatccc tatgggcaca gccaagcctc gtccggcaga ctggtcaccc
     1921 cgccgcgaga ggcggttcta cacgagttca atcgcgccca aggtgtgtac gagcagctca
     1981 aagcggccgg caagataagg ctcgtcttcc ccgatgaccc tcgtgctggc ccgggtaccg
     2041 aaccggccct ccaggccttt ctggctgcat tggtggggcg gcaaggcccc ctcaggcccg
     2101 ccggcccttc tcctgtccct tcgcagtggg cagacgccga tgttgatgcc cgccaggctc
     2161 gccagtttgg ccaactggtc ggttttacgc agagactcgt ccgcgaatcc ggcgagcgcc
     2221 gccgtcagtt ctggtcgaag gccggcgcct cctcgctcga gaagtggcag aaatcgtgcg
     2281 agtactatcg ccgctacttg tgggacgagg tcattggccg atgccccgcg cccggcgaca
     2341 gcatcaaccc tcgctcccgc cggatcatcg acaacgacaa gtgggcgggc tatgaagttg
     2401 ttctggacgt ttatcccgac gtattcgcat atggaatact gttacttcct aaggacattc
     2461 ggcccggcga gcgaaggccg gttgtcgtgt gccagcacgg cctggagggt cggccgaaca
     2521 aggttgttga tcggaccatc aaatcgccct acaaagcctt cggcgctgac ttggccgacc
     2581 gcggttacat cgtcttcgcc ccgcagaacc cctacatcgg cggagacgcc tttcgtcttc
     2641 tgcaacgcaa ggccaacccc cttaagcttt ccctgttctc gttcatcacc gcgcagcacc
     2701 ggcagattct cctctggctg ggacagcaac cgttcgtcga tccgaagcgc atcgccttct
     2761 atggcctctc ttatggcggc aagacggcca tgcgggtacc ggcgctgttg cccgaatact
     2821 gcctgagcat ctgctcgggt gatttcaacg agtggatcgt caagaccact tcggtcgacc
     2881 tcgtcaacag ctacatgttt acgtacgagt acgagatcta tgagttcgac atgggcaaca
     2941 cgttcaacta cggcgagctg gccggcctga tcgcccctcg gcccttcatg gttgagcgag
     3001 ggcatgacga ccccgttggc atggacgagt gggtctcata cgaatatgcc aaggtccggc
     3061 ggctttacgc gaagttgggc ctccctgacc tcacggaaat cgagtacttc gacggccggc
     3121 acgagattca cgcggtgggc acctttgcct tccttcacaa acacctcgcc cggccaaggt
     3181 agatcgggct ccgcccgtca tgggcactat gattgactca acagcccggt tcaagacctt
     3241 acggccggac gtctgcgcgg cttgcgcgca tgtttccagc tcgcagtgcg cttgggttgc
     3301 ggtaccccca agggcggtcc gcctcagaga accagggctt gagccggatc ggccagctcc
     3361 ctgtacttgc cgtcaggctg ccgaacaaga acaacttcgt ggtcttctcc aaattggccc
     3421 tgtaccttgg cggtaagtct ctggtgggta tcgtgctcat ttggctgttc agatggcttg
     3481 ctcgatcggg cctgttggtt cccgcacgat cctccctcta cgctgtcggt cggcagttcc
     3541 cgggtctcat tcgcctcgac gtaatggccg tcgggctggc ggaccagaac gacggtacgc
     3601 tcaatgccga agcgtccttc cacggtggcc gtcagtgttt gtggcttgct gtccatggta
     3661 cacccctcta cggattacta tgctggttct agccgatatc tccttgctgc gatcgtcacg
     3721 cgccggcggc acgtcacgcg atcgcccctg cggcgatgga cacggctatg cggcgccatt
     3781 gaaatatacg attcgctctt atgcttcggc cagttcatcg tggcctgcgt tgctcgaaat
     3841 gctccattgc ttgcagagaa catagctcct cgttgtcctg ttattggacc gcctgtctcc
     3901 ctggttgacc ggtcggctct cgtttgtccg ataaccgtat ctgccgaact ctctcgggcc
     3961 gacgctccga agcgccggcc ggcgggcatt ccagatgctc agcttgtcat tacgggcaca
     4021 cggaccgctt ctcgagtcgc cgacggcgaa caagcatcac acgccccgac caggtgaatg
     4081 agcattgcgt tggtgctgcc gggttcgcgg agatgggtgc ccgcgacgat catgatcata
     4141 tcgtttgtct tcgccaactc gcgatcgatc agcacggcat cgacgtccct gatcatgtct
     4201 ttctgtcgtg tctgatggtc aagacgcgca ggccgaacac cgtaataaag cgacatgcgc
     4261 cgacagacgc gagcatcagg actgaggccg acgatcatct gcgggatgcg gcgtttgctc
     4321 agaagtcgaa ccgtcgtgcc cgtgtcggtc cacacggcca gcgcatggac gtttaactcg
     4381 cgagcgagga cgcttgcccc gtggaccacc gcggatacga actgcaactc ctcgggatcc
     4441 acacggggga cggagtctcc tgcctggccg gcgagatacg cctcggtctg gtcggcgatg
     4501 ctgcgaatca cgcgaacggc ctcaagcgga taaccgccga ccgacgtctc cgccgaaagc
     4561 atgaccgcgt ccgcgccatc caggatggca ttggcgacgt cactcacctc ggcccgcgtg
     4621 ggaacgggtg attcaaccat gctctggagc atctgcgtgg ccacaatgac aggtttgccc
     4681 gcacgagcac accgctgcgt gatctgtttc tgaagcaatg gcaccctcga gacgtccatc
     4741 tcgacgccca ggtcgcctcg ggcaacaagc aacgcatccg acaggctgat gatttcgtca
     4801 agatgctcga tcgcctcggg gcgctcgatc tttgacacga cgtgggcgtt gctctcgtgc
     4861 ctcgtaagct cggctcgaag ttcctccagg tccttcgggg agcgaacgaa cgacagggca
     4921 aggtagtcaa gatcctgctg agccgcccat tggatgtccg ttcgatcctt ctgagtgagg
     4981 gcaggaagcg agagtcggct atccggcagg ttgacaccct tgtgggtgcg aatggtcccc
     5041 ccgacctcgc attcgcagat cagcttcgcg ccttgtttcg ccaccgcccg gagccgaatc
     5101 tggccgtcgt cgatcagaac ccgctcgccg cactggacat cgtcgcacaa ttgatcatag
     5161 cttgtacaga ttcgtccggg cgtgcatgga aaactgcccg gctcgatcgc aagaagctct
     5221 cgaacggcaa tgtcgaagct tccgtcggca atctgcccca cgcgaatctt cgggccgcag
     5281 aggtctccca tgatcgcaac cacatcaccg ctttgcactg cagcgcgtcg aatggcggcg
     5341 acggtctggg tgtgctcttc caaattgcca tgcgacagat tgacacggaa gacgtcgacc
     5401 ccctcgtcga tcaaggcgcg cagcgtggct tggtctctgc aggccggtcc gacggtcgca
     5461 acgaccttcg tcttgttgct gtgtgacaag gtacccgtcc tttcaaggcc cgcgtttgga
     5521 ctgactgcgt tactgcgaca ggcaagacag ttcatgcgat cgagccgatc tcgatcacct
     5581 gccggccgca gattgtcctt gccggggtag cgggcgtgtc tgtcgtcgcc ccagttcccg
     5641 gttcatgccc gaagcgccac gtgaacaacc aaggcccgtt cttgctacag gcggctgcac
     5701 gcaccgacag tccgggtcgg agaccggcgc gcacttggtt cctcggggcc ttcatcggtc
     5761 agaatgatcg ttgcctcaag tacggcgcgt cccataaagg tgccggctgg cttgccaggg
     5821 cgattccggc tagattgccg cagagattga ccggctttcc cgcacacgga ggatgcgcat
     5881 atgcgtttca accgttcctg cctgccgata ctgtggctgc ttgttccact ctgtcttgat
     5941 tggccgcccg cgtctgccgg ccaggagccg ctgaatcggc cgaatattct ctttctcatg
     6001 gccgaccagt tccgggcgga ttgcgttggt gccgacggaa atcctgtgat ccgcacgccc
     6061 aatctggatc gaatcgccgc cgaaggtgct cgttttcgcg ttgcgtacac atcgacgccg
     6121 tcctgcacgc cggcccggtc agggatcctc accggtctgt cgccctggca ccacggcatg
     6181 ctcggcatgg gccgcatggc gctgagctat cagaacgaga tgccgcggat gctccatgag
     6241 gccggttact atgccgccgg catcggcaag cagcactttc acccccagcg gaacgcgcac
     6301 ggctacgact tgctgatcct ggacgagtcc ggccgggcga cgtcgcccga ttttcgcagc
     6361 gactatcgaa gctggttctt gtcgatggcg ccgacactca accccgacgc caccggaatt
     6421 ggctggaacg actaccgtgc cgcggagtac gcactgcccg agtctctgca cccgacacgc
     6481 tggaccggcg atgtcgcggt caacttcctc aaggagtatc gccggcccga gccattcttc
     6541 ctgatggttt cctttgcccg accgcacagc ccgtacgacc cgcccgggcg attcatgaag
     6601 atgtacgaag acgctgatgt ccccccggcc gtcgtcggcg attgggtcag ccgccatgct
     6661 ggcaaggaca accccgagga tttcaactcg tggcggggca acttcggccc tgagcaggtt
     6721 cgtgcgtcgc ggcgtgcgta ctacggctcg gttacgttca ttgatgagca gatcggccgg
     6781 atcctggcca tcctgcagga acgcggctgg ctcgacaaca cgctgattct cttcaccgcc
     6841 gaccacggtg acatgctggg tgaccaccat ctctggcgaa agacctacgc ctacgaggcc
     6901 tctgctcgca tccccatgct gattcgatgg ccttcaagca tcaatgccgc ccgtgggcaa
     6961 gtgctcgcac aaccggtcga acttcgcgac ctgctgccca cgttcctcga cgcggccggt
     7021 gcgaccgtcg acgaaaagcg tttcgacggc cgaagcctgc tgcgtcttgt ccgtggtcgg
     7081 tcggacggct ggcgcgagta tatcgatttc gagcacagtg catgctatgc gggggccgga
     7141 tactggactg ccctgaccga cggcaggacc aagtacatcg ttcacgccgc cgaaggccag
     7201 gagcaactat ttgacctgca ggccgatccg ggcgaactgc acgacctggc ggcggacccc
     7261 gcacagcagg acaggctgcg cgcctggcgg gaacgcatgg tcacgcactt gtccgagcga
     7321 ggcgagcctt tcgtgaaaga gggtaagctg gttctgccga ggccgtccat gctctacagc
     7381 cccaatttcc cgaaggatga aggcgaagag aacagcgccg tcggcaaagc gagacaatcc
     7441 gcccagacac ggccgaccgg taggccgcgc aggtgaaaag agccatggag caatgttgat
     7501 gagtgtttca accggaagtg cgatcggagt gttgtgcgga ctggtgtcca tccttttctc
     7561 tccggatgtt caggctgtgt ctgcttccac ccccgcccgg cctcgcgtgt tgatcatcgg
     7621 agactccatt tcgatcggct acaacgaacc ggtgcgcgag atgctgagcg acgtggcaga
     7681 tgtcgcacac aatccgggca acgcggcgga tacatggaag ggcctggagg aactcgatac
     7741 ctggctcggc tcggagaagt ggagcgttat ccatttcaac tgggggctcc acgatctcaa
     7801 acatgtccgg gatgggaaac ttgacctcac cgctccacgt gtctcgacga ctgaacagta
     7861 tcgagagaat ctcgacaagc tcgtgcttcg cctcaaggaa tccggcgcca agctgatctg
     7921 ggcaagcacg acgccgatcc cggaaggtgc cgccggccga atcaagggcc aggagatcga
     7981 gttcaatacc gcggctcgcg aagtcatgga ccggcatggc gtgacggtca acgacctgca
     8041 cacctgggta tggccccatc tgggcagata tcagcagaaa gccaatgtcc atttcactga
     8101 agaaggctcg cgttttctgg cggagagggt tgcccgcaag atccgtaaca ccctgcagga
     8161 tcagaggcct ccgtttgaga tgcccgcggt gcaaccgccg gtgtttcccg atcgccacgt
     8221 gagcattgag caattcgggg cggtgggcga cgggaagact ctcaacacat cggccattgc
     8281 ccgagccatc gccgactgcg ctgagaaggg tggcggccgg gtcctgattc cacccggcgt
     8341 ctggctgacg ggcgcgattc atctcaagag caacgtcgag ctgcatttgg ccgaaggggc
     8401 cgaattgcgg ttcagcacca acccggccga ctatctcccg gcggtgtttg ttcggtgggg
     8461 gggcttcgag tgctggaact actcaccgct gatctacgcg agagactgcg tcaatatcgc
     8521 tgtcaccggc cgtggcaaac tggagggcca gggaaaggca tggtggggtt gggtcaaaga
     8581 gcaggatcgc gtctcgcgaa ggctgtatga catggtcctg gcaggcgtcg atgtggagca
     8641 acggcttttt gccgacgaaa aagagccgct acgcccccaa ttcatccagc cgatcaactg
     8701 ccgaaacgtg ctgatcgagg gggtcaccat caccagcggg cccttctgga cgatccaggc
     8761 ggtctactgc gagaacgttc tggttcggaa catcaccgtg acgaacgagg ggccaaacaa
     8821 cgatggattc aacgcggatt cgtgccgcaa cgtcttggtc gagcactgca cgttcgcaac
     8881 cggtgatgac agcgtggcga tcaagtccgg attgaacgaa gatggttggc gagtggggcg
     8941 gccctctgag aacatcgtcg tgcggcattg ccgtatgatg cgcggacacg gcggcgttgt
     9001 gatcggcagc gacatgtccg gcggcgtgcg gaatgtcttc gtgcatgact gcgatttcag
     9061 cggatcgcag atcggcattc ggctgaaatc gtcccgcggc cgtggcggca tcgttgaaaa
     9121 cgtgtattat cgcgacatcg atctcggtga catccaaggt caggccatca ccatacatac
     9181 cgactacaag gcctactttg gtgccgatca gggcaaggcc ccggcgttcc ggaagattca
     9241 catcagcgat gtgatctgcc gcaaggccgc ccaggcggtg cgtctgtcgg gtctgcctga
     9301 acaattgatc gaggacgttg tcttcgaaag agcgatactt ggcgccaagg agggcatgac
     9361 ctgttcacag gtgaagggtc ttcgcttggc cgacgtggcg gtcactcccg ccgccggccc
     9421 ggtgatgcgg gtcaaggaca gccggcaggt tacgctccag cgagccgtgt gtcctcgcga
     9481 tgccgatccg ttcctgcggg tggaaggggc cgggagcggt gagattcggg tgatggaaag
     9541 cgatttgtcg ttggcgaaga agagcgtcga gttggccgag ggcgcaccgg ctgatgcggt
     9601 cgcgatagat gacaagccgg tgcgatgacc aagcgggcgg caacgttcgt gccccgaagg
     9661 gggcggcaac gttcgtgccg cgaagcgggc ggcagcgttc gtgccccgaa gggggcggca
     9721 gcgttcgtgc cccgaagggg gcggcagcgt tcgtgccccg aaggggacgg cagcgttcgt
     9781 gccgcgaagg ggacggcagc gttcgtgccg cgaaggggac ggcagcgttc gtgccccaaa
     9841 ggggcgcagg tatgtagcca ggggcgcaag cccctggaag cggtgtcaca ttgaccagtg
     9901 agccccagcg gggcgataga agctcacttg tcacggaaag cccacaactc gacgccgcgc
     9961 atggcagacc ctaaccatgt cacttgtcgg tcttggcgaa gaacacagtt gccccattct
    10021 ttgccagtga tgcgatctcc tcggccgaca tggctcggcc cagcaccagc acgtcatcca
    10081 cgttgcccat caattgctcg tcgctgccgc gatgtgcgtc ctcgccgatc agcaggctgc
    10141 gagcgagctt taccggctcg ccgccgggta tccattgtcg accgattggc ttcccgtcca
    10201 gatagaacgt cacgcagccg tcatcataca ccacggccag gtggtgatac ttgccgtcgt
    10261 tgaacgtcac ggcctccgaa tccactgcga tgcctttgca caccaggcgc agaccgttga
    10321 gcatccggcc gctcgggtca aaatcgaaga tgagctccgc gctgctcacc gggaagttgc
    10381 cgttgtacgc gctgaacagc cgagcatgct tgttgttgct gctcttgacc atggcggcca
    10441 acgtgaaata cctgcccagg tcatccgtgc cgagaatctc cacgcggttc cagggacgcc
    10501 attgcgaccc gaccacgagg gacctccggc cgaaagccgg atccgggttc ttgtgatcga
    10561 gggtggacgt tcccaggtta cgcagcgcct gaagcccgtc ctcgcccagc atgtccgacc
    10621 aggcttcagt atcgccctcg aacgtgtata gagcctgcaa aaccgggacc gggggctcgt
    10681 cgatcgactt ggcgtgttga cccgccatga aggagtagag acggccgcga tccaggatga
    10741 accggaatcg aaccgggact ccgttcggca gctcttttct tgtcttccaa ctgacaacgc
    10801 cgcggatcgc atcgccggtc agtggttcac agtcctcgcg cccgtaaccg gcgacaacca
    10861 gaccgtcgtc gccgatcagt tccactcgaa tggaaccgcc tcgcgcctga tagttgacca
    10921 gaagcggtcc ttcagcattt tcaaatcgct tggtcaatac ctctcccggc agttcgtccg
    10981 cgtccagcga agcaaagccg tccttgcgca gcttggccag cccgatcgcc gatcgatacg
    11041 gcagcaggtc atgttcctcg tcatagccgg tgtagaacgt ccacaactcg tcgcccacca
    11101 acagcggcgt atgggcgatc accatgcccc ggtcgaactc cctgtacttg ccgatgtcca
    11161 gcagtggcgg gcggctgacg ctgtccagtg cgtagatgtt gcgcacggag gtgtccgcct
    11221 cttcgcgcaa ccagtgcacg ccatcacggc tgctcaccag ttcgagccac agcggcccat
    11281 ggaagtagcc ttcgggctcc tcggcccgat agatctggag caggccgata tacatcgtct
    11341 cgtacggtaa aaccgggcag gcataaaaat gggtccgctg gaccgtctct ggcttgcacc
    11401 agaggtcgtc cacgtcatcc ggagccatga ccatccgcag cggagggaac ttggtcaaat
    11461 cggtcgtctc gctgaatccc acgacccggc ggcggagccc gctcacgtcg ccgcttacat
    11521 aggcattggc cccgcccttg accgtgcagc ggaaccgctt cgtatgcgcg tcccaataga
    11581 aatggcttgt gtcgccgccg gaaatgatcg tctcggtcga ctcctgcttc caattcaggc
    11641 cgtccggcga cgagaaagca tgatacatcc ccccgacgcc ctggtaacgc ttctgtggat
    11701 ctttctccca cggagtgtac atgacatgcc ccggacagtg gggaacgatg ttgttgtttt
    11761 tcgttccatc accgctccat tcatacaacc cgagattggg cttctcccat ttgagcccgt
    11821 ccttgctcac cgcatagcac atgaacgagc cacgcccctt cttcgaagac ttgtcttccg
    11881 tccagcagta gtaccacatt cgaaagcccg tgccgtcctc cccgggcagg accgtgaccg
    11941 cattgaccac gtgcgcctcc catggcctgt cgacccgaat gagcggattg tccgggtgct
    12001 tgcggaacgg gtgataccga cgcacaacgt tcttcttgga tacgatgaga taatcatcga
    12061 tgaacagttg ccacgggccg gtcgtctgtt cagtggcccg cacgggctcg ccggccaggg
    12121 cccggcccac gccgattact gccgagacaa aaagaaacag gatcgccaag taccggatga
    12181 ggtgagtcat gatgtcctcc aggcagttgc gaacgacttc cgatgcccat gaccggtcaa
    12241 cagaataaga ccaatcttgg cgacaaagca agggctcagg ttgatgcgtt cgcgcagcct
    12301 ccgtctgccc gggggaccag ccagatctac gacagtcccg ctcgcaatcg tgcgagaaga
    12361 atccgtctgg cttggatgca acaaccggat cacgtaatcg tttacctttt gtatgagagc
    12421 ctgcagagag ctgcgagctg gaggcgcatc gagttgcacc cttctcgggc tggctgagtg
    12481 gttatccggt ggataaggcg cgattgttct cttaacgtac tgtcctgaaa agggttacaa
    12541 ccttgtggct ggacatctgt gcgtcttgcg ggcacgtttc cggatcgcaa tgctcttaga
    12601 accttacggc cagatatctg tgcggcttgc gcgcatgttt ctggatcgca gaacgcttgg
    12661 gttgcggtcc gcaaaaaggc ggtccgcctt aggttccggc ccggaaaaag gcagttcacc
    12721 ttgggacttt cggcgatgga ttggcaaatc gcagcagggg tcgttcaacc gattgtggcg
    12781 tttgggcatc cgaccttacc ataacaggtc agaatgcatg aaggagacaa gacatgcttg
    12841 gcatcacacg tcgaacgggg tcggtactgg tcgggatcct gtgccttggt ctggttccca
    12901 tcgcaatcgg ccagcaggct gagcagaatg gcgaccaagg ctcgcgtagg cggggtcctc
    12961 gcggaggccg gcaggataac cagcagactg ctcagcaggg cgagcagcaa gacggccagc
    13021 gcggtgggcg gcggggtagc cggcgcgaag gtcagcaggg ttttcaggag ggcgggccac
    13081 gtgatcgtca gagtggcccg aggcaagagg gggagcaagg ccccggacgt ccagaccgtc
    13141 gcggcccgcg cacgttcacc cctgaagacc gcgagcgatt ctataacgag atggtcaatc
    13201 gttatatgga acggctcagc gatacgtatg aactcagcga cgatcagcaa acacaagtcc
    13261 ggtcgcgcct ggaagaacta cgcagccaac agagaacata ctccgagcag catcagaagg
    13321 agttcgacga tatccggaca cagatggagc agttgagaga aagcggggcc ggcttcggat
    13381 cgccgcaagc tcgggagttg tttcagaaaa tggaaacgct ccgcgagcaa tcgccgctga
    13441 tgaaccgtga cacggtgacc gccgaggtcg agaaactgtt gccgccggaa caagttgaga
    13501 aaggccgcgc ccgccgcgag gcagagcgcc aggaatggga ccgtcgacgg gaagaaatgc
    13561 gacgtcgttg ggaggaacgc aggcagcagg agggcgacca atctcagtcc gacgatgacg
    13621 gtccgatgcg gttcttcaga gaccgcggtg accggcgtcg gcgcgatcgc ggggaggagg
    13681 gtcaggggca accggtcgag ggacaggaga accaggaaca gcaggggcca gaaggccagg
    13741 accagcaaca gggccaatgg ccgcagattg acgggcggcc agtcgtgcag tggccggtgg
    13801 aagaccccct cggtccgtgg gatcgctatg ttcgcgactt tgcccgccgg tatcgcctcg
    13861 atgagtcgca aagggcaacc gctcaatcca tcttgcgcga gatgcacgaa cgccgccagg
    13921 tgtacgagca ggcacaccgg caggattatg aggcggccca ttcgcttcaa gggcaggaac
    13981 gggaaaagcg gttggcccag ttgaatcagc ctattgagtt ccttttcagc cagctcaaga
    14041 gtcggctgga gcgaatcccg accaccgagc agagaagggc ggcaggtgaa ctcagaacca
    14101 ccagccggcc ggcaactccg ccatcgtcgg ccccggccgg cgcgacgtcg cagccggcgg
    14161 gcggatagaa gggcatcatc cgaggatcat gagctgccgc tgcgttgcga tcggaatgct
    14221 cgtatcgaag gccctcgagc ctggaagctc gggtagcacc tgaattcccg cattcaccgt
    14281 ccggttcggc gcgaatcatg cgctcgacag gcgattcagt gtctccattc cgtaaatgag
    14341 cgctcggggc aggtggaacg gatccttgac cggcagggcg acgacatccg tgatcgggtg
    14401 gccccggcga tcgagtttct gagtccattc accgtgaggc ttcacgggaa aatggctgaa
    14461 gctatagtcg tgaagtctct ggtaccattc gagacaccag tcctgccgcg aaatctcgta
    14521 cgccaacagg gtcgcgtaga gggcttcggt gtgaggccac cacagcttgg cctccggaaa
    14581 ccgccaggcg acgtccgaac gaccatcggc gtcgactgcc aggaacaggc cgccgtactc
    14641 agcgtcccac ccgatttcaa gatggcggcg aacggcctcg accgcccgct cgatgatggt
    14701 cgattcaccg gtttccaggg cgatgtggag ctgcatccac atcgactcaa tcgcatgacc
    14761 gggcagaacc acggtacctt cgggaggggc gatttcgttg ccggccatgt ccaaccactc
    14821 cagaatcagt ccccgcgcgg gtcgaaggaa ggtttccata acgagtctgt ggtggcgtag
    14881 actggcagcc ttgaccgacg ggtcgcccgt cgcgtccgcc agttcatggt ag
//
LOCUS       DAOTPW010000138        14792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3967_length_14792_cov_4.897537, whole genome shotgun sequence.
ACCESSION   DAOTPW010000138 DAOTPW010000000
VERSION     DAOTPW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14792
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3967_length_14792_cov_4.897537"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /locus_tag="P5316_13130"
     CDS             <1..1633
                     /locus_tag="P5316_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRR85946.1"
                     /translation="YLRDTLLSLCAVDTAPDSDLASTASREQQLFDLIQREVEGLRDC
                     SGEVERPPIDPAIESEPAYTAPGYAADRRGRVPPAEQVYAGRTNLVVIFDGNPGTNPR
                     PVVQHAHVDIVPFWTAPRAVGHRVVGRGACDNKAQVAVLLAQMRLLDELQRHLGRQGR
                     RGRVYQFVIDEEIGGNGSLSLAMDPRFSGCPMIVHECTGLVPYCAHRGCVYYRCRLSV
                     GNGTEGNALKMVPYVVTALDEEGRRFQEETRHPLFGLEHVQANPGILGPYGRTPGTVC
                     DHVAVVVALGSQASPEEAAVSIERCLADSLRAYCRRHGDKTVILDAATGKPMLEKHFS
                     VECVSQSGPPGFRIDVFGVGGHMGALSECDNAITKAAYMLRGLLDSADIPLRDVIVRL
                     ADADEEAAEVVLEGGQGFTPCHRMEDIEARLAAAARRGAMDCSAACGFRFDESMIEMS
                     FDRLHNDAYAADPGIEPMRALLAAREAVGLPPVEVVGWRTSCDARLYHARGYPVAIFG
                     AGRLDVAHSDHEYVDIAEMQQALAISTLAVWSMMM"
     gene            complement(1686..2315)
                     /gene="cysC"
                     /locus_tag="P5316_13135"
     CDS             complement(1686..2315)
                     /gene="cysC"
                     /locus_tag="P5316_13135"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRR85947.1"
                     /translation="MTEIKATNITWHEGHVSRADREKLLGQKGATIWFTGLSGSGKST
                     IAFTVEHALVERGHLAYVADGDNIRHGLNKNLGFSAADREENIRRIGEVAKLFADAGC
                     IILTSFISPYRKDRDQARKIHEEAGLAFIEVFVDVPIEKCEQRDPKGLYKKARDALAA
                     GKGMGFTGVDDPYEPPLKPELVIHNDKVTPQEAATQVLDYLQQKGLLTK"
     gene            2463..3446
                     /locus_tag="P5316_13140"
     CDS             2463..3446
                     /locus_tag="P5316_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR85948.1"
                     /translation="MSEASRLKIVVTERASEAAMARLAAVGDVVVLPKCDEASLIEAA
                     ATADAMVVRTYSLINAKVIEAAKAGGRLKVIGRGGVGLDNIDLKAALDAGIKVVYTPA
                     ASTHAVADLVVGLIIALQRRVVENDKGMRGGEFDSIRSVRPRAKELRHQTVGVIGMGR
                     IGTAVGFRLANGFGTRIIYHDIREIGWLPFPAESKSSHEAVYAEADVVTLHVPLTKLT
                     RGMIDADALASFKQGSYLVNAARGPVVVARALAEALESGHLAGAAVDVHDPEPPPADY
                     PLLAAPNCVLTAHIGARSPEGLAAMDDVVDDVIAVLQGKEPNWPAGPELIE"
     gene            3767..4702
                     /locus_tag="P5316_13145"
     CDS             3767..4702
                     /locus_tag="P5316_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR85949.1"
                     /translation="MSTYLVTGGGGFIGSHIVHRLAADGQRVRVLDNFSTGKRANLAA
                     VSKDIEIIEGDLRSMDDCRKACEGVEIIFHEGALPSVPKSVADPRSSHDCNINGTFNI
                     LMAARDCGCRRVIYAASSSAYGDQVESPKRESMRPAPLSPYAVNKLVGEYYLKAFHLC
                     YGLETISLRYFNVFGPRQDPKSQYAAAIPAFVTAILNDKPPTIHGDGEQTRDFTYIDN
                     VVHANMLAAQAPRLSGEVINIACAESVSVNRTIRLINELLGKDVKPIYTDVRPGDVKH
                     SLADISLARKIIGFEPVVKFDEGLRRAIEWYRGNL"
     gene            4847..5512
                     /locus_tag="P5316_13150"
     CDS             4847..5512
                     /locus_tag="P5316_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRR85950.1"
                     /translation="MRRKTRKASGTRRPDIWLPFWRRRGVGLVFLLVLATVILFERFG
                     LFGGPGGREQPAAIIAGTDHDRYNNRTFTCIKVIDGDTLYIDAPDGNKSYTSVRLIGV
                     DTPEVDESLGRPTYFGAEASAFTRSQAEGKPVRIVLKEKETRDRFRRLLAYVYLGDGT
                     TMLNEEIIAQGYGYAYTTYPHPWTPRFIDLERRARKQKRGLWKEVTTERMPQWRRKLE
                     EPR"
     gene            complement(5529..5873)
                     /locus_tag="P5316_13155"
     CDS             complement(5529..5873)
                     /locus_tag="P5316_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85951.1"
                     /translation="MLERYRSLEILAGGLALFVIVLPGQWPTFSSGCVVVALCALAAV
                     ALLLEFLARTEVRRIERSAVEYDNSLCLICGYCLKHLPETHMCPECGTQYDLNHVVAE
                     WQAYFISRKRKR"
     gene            complement(6066..6566)
                     /locus_tag="P5316_13160"
     CDS             complement(6066..6566)
                     /locus_tag="P5316_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85952.1"
                     /translation="MPSRLRYIGRVISLFFRLFIVRSAKVMFLPKSGPKGAPVPPPVI
                     KRLENRPQMVAGALLVFLFFLLNLLYFLHVDPVQHAHYAVYIVGGVFVICFLILPWSR
                     KEIVVLLREAGANDCRLCLHCGYNLKGLPAQHRCPECGSPYEIEQVKRTWSEYLADRA
                     AGHTLL"
     gene            6991..7941
                     /locus_tag="P5316_13165"
     CDS             6991..7941
                     /locus_tag="P5316_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRR85953.1"
                     /translation="MAHTGLVLSERYEDHDTGPHHPEAPERVRTIRLRMRESGLADAA
                     LLIDPRPADPSVIDPVHPPEYVSRLRHACEMRAGFIDTPECPVCDETYEIALMAVGGL
                     LQAVDAVMEGRIRNAFCAVRPPGHHAERRRAMGFCYLNNIAIAAEYLRSHHGLSRVAI
                     VDFDVHHGNGTQHQFERDPNVFFCSIHQDPNTLYPGSGFEWERGQGDAVGTTLNVPMA
                     AGSGDAEYKRAFEEVILPAVADFKPEFILVSAGFDGHRGDPLAYIQLSTACYHWMTHR
                     LVELAGEVARHRLVSTLEGGYNLDALADSTQAHVEALMNG"
     gene            complement(8044..8571)
                     /locus_tag="P5316_13170"
     CDS             complement(8044..8571)
                     /locus_tag="P5316_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85954.1"
                     /translation="MSAVSPISGYGGSLPTQPPQCTQPAQLGGVDGGQAAKAGGPIAG
                     ASVSAGQSAVAISQTSISAGVESFVSTYGPVLADNQILGAMLLLLMLEYMQSDDDDEK
                     KGLLGLMLSLLQQQQASQAGGTFIYSSSQFSLQSTQMVAVSQQLAVDVYAGGGSAGAA
                     APVSGPAGGGVDVVA"
     gene            8961..9539
                     /locus_tag="P5316_13175"
     CDS             8961..9539
                     /locus_tag="P5316_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRR85955.1"
                     /translation="MTDGREQGNRGSLTVVVGCMFSGKSEFLVSQLEEAARRGRATAA
                     FKHSSDNRYCHRDIASHTGRRFRAMAVPEASRIADLAKVAEVVAIDEGQFFAEDLVDV
                     CRELVVAGKEVLVAGLDLDSWGLPFGPMPGLAGTADRVIRLQAVCARCGRPADHTQRV
                     APITGQKMVGGADCYEPRCAACFEAPPIEKRC"
     gene            complement(9696..11195)
                     /gene="dnaA"
                     /locus_tag="P5316_13180"
     CDS             complement(9696..11195)
                     /gene="dnaA"
                     /locus_tag="P5316_13180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRR85956.1"
                     /translation="MITPRMDSVALIQSTIAQEVGPQRYKVWFKNATQFTMTDGHLKI
                     STPNAFICGWIERHFADTISDAARRATGQDLQLSFVVDPNLLHALRKRQLNSQADFIA
                     KNPDRVARRHRHEPTTPLSRLRGRFDSLVVGPSNELAFSVVKRIADRDPAAYSPVFVH
                     GGCGVGKTHLMHALANHLAEHHPDCRWLCVTGDDFTNGFIHALQTKRIEAFRQQYRNV
                     DVLIIDDVHFLANKKATQEEFLHTFNAIDGHRKQVVLASDSHPKLIGQFSEQLVNRFV
                     AGMVVRIDVPDYRMRCEILNRRARVATSQPVPQEVIAYIAEHLQANVRELEGALLKLI
                     MFSRVAHQPITLSLARHALADHLARTQRALGLDDIDHIVATYFGLTPADLHTSRKTRT
                     LALARNIAMYLARKHTRMSFPEIGRFMGDKNHSTVILANRKIERLLAADEIVARQTPA
                     GIKEERLRDIITSIEEQIARQRTSQHPRPSSEPATPDSGVVRQPRVAIG"
     gene            complement(11499..12437)
                     /locus_tag="P5316_13185"
     CDS             complement(11499..12437)
                     /locus_tag="P5316_13185"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR85957.1"
                     /translation="MVISTPATDRELQRGLQRLVYHPKLSPAEIDRRVKTLVNHAERR
                     GLSLKHCLIARHERNILYSCLCIDSPGRSSAVYLPGWLPDPQAAETLVSLLRKAADEA
                     RNRGISFLQAIIPLDAALERNAYEQAGFQRLAELIYMERESTEPLVVEKPSPALSWIT
                     YSPETHQLFAQTVQDTYEGSLDCAGISGKRDIEDVMATHRCTGVFNPRTWFVGVADGR
                     PVGTLLLSEVPEQAALEVVYMGLRPGYRGKGYASTLLEHAVRTARNRAALRLTLAVDA
                     ENSPARRLYARFAFVETSRRSAWVRILSPANKPPDR"
     gene            complement(12519..12989)
                     /locus_tag="P5316_13190"
     CDS             complement(12519..12989)
                     /locus_tag="P5316_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85958.1"
                     /translation="MIAALVGATTLLGCRLDTASPTTVPAGTEELPILWQASGTYSRL
                     TKAVRLVVRDPATLAQIPLCEVPVDFRTEMILIAGLGPTPRDDCGIIIQHVWKEGDRL
                     RVLERQIYPGSRIRPGFRLASPWTVVVVPRCDLDVEGYSSRLPRGTFLSANPPR"
     gene            complement(13204..13647)
                     /locus_tag="P5316_13195"
     CDS             complement(13204..13647)
                     /locus_tag="P5316_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85959.1"
                     /translation="MRSRTRLVGSCIGAMMFLPLAGCSPPTVNSNGDRGTVLSTLTKL
                     TDPVKNIGALNSAELQILVTQLPTLTDELAKLGFYLPPDIALPDLDDQEAEALEQFLA
                     ANGVNTFEDLADLASAVTAGLVQIPEILQDDFDAFAAQFGRPRPT"
     gene            13953..14714
                     /locus_tag="P5316_13200"
     CDS             13953..14714
                     /locus_tag="P5316_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRR85960.1"
                     /translation="MMDDRQLDIVFAAPHPDDLEIGCGGTIASLADKGYQVGMVHLTN
                     GEPTPNGSPERRSQEAVKAAEVLGAAVCEILPLTNRELMDGPPARYALATILRRYRPR
                     ILVGLAGRTTAASPDHYQAQLIVEAARFYSQLTKWDDRFGHTSPHRIDHLVYRPIPSA
                     AERMHFHGQFVVDITDTLERKLEAIRCYRSQFDGQRFDRLRHYVTSVAGAEGGQAGYR
                     YGELYCLPRPVGVKDMIAMLGSWEIPPPFSPYSSG"
BASE COUNT         2740 a         4045 c         5033 g         2974 t
ORIGIN      
        1 gtacctacgt gatacgttgc tgagtctgtg tgcggtggac acggcgcccg attcagactt
       61 ggcctcaacc gcatcacgag aacagcagct ttttgacctg attcagcgag aagttgaggg
      121 cttgcgtgat tgcagcgggg aagtggaacg tccgcctatc gaccccgcga tcgagtcaga
      181 gccggcgtac accgcgccgg gatacgcggc tgatcggcgg gggcgggtgc cgccggctga
      241 acaggtctat gcaggccgga cgaacctggt ggttatcttc gacgggaacc ccggtacgaa
      301 cccacgaccg gtggttcaac acgctcacgt ggacattgtg ccattctgga cggcaccgcg
      361 agcggtgggg catcgcgtcg tcgggcgcgg ggcgtgcgac aacaaggccc aggtagcggt
      421 gcttctggca cagatgcgcc tgttggacga gttgcagcgt catctggggc gtcagggccg
      481 gcgcgggcgc gtgtaccagt ttgtcattga cgaggagatt ggcggaaacg gctcgctgtc
      541 gctggcgatg gacccgcggt tttcgggttg cccaatgatc gtgcacgagt gtacggggct
      601 tgttccctac tgtgcccacc gggggtgtgt gtactatcga tgccggttgt cggtcggcaa
      661 cggcaccgag ggcaacgcgt tgaaaatggt tccgtacgtt gtcacggcgc tggatgagga
      721 gggtcgtcgc ttccaggagg agacgcgcca tccgctgttc gggctcgagc acgttcaggc
      781 gaacccgggc atcctgggtc cttacggcag gacgcccgga acggtctgtg atcatgtggc
      841 ggtggtggtg gctctgggtt cgcaggcaag tccagaagag gctgcggtga gcatcgagcg
      901 atgcctggcg gattcgctaa gggcatattg tcgcagacat ggcgacaaga ccgtcatact
      961 tgacgcggcc accggcaagc cgatgcttga gaagcacttc tcggttgaat gcgtctctca
     1021 gtcagggccg cctgggtttc gcatcgatgt tttcggcgtt ggcgggcaca tgggtgcttt
     1081 gtctgaatgc gacaacgcga tcaccaaggc agcgtacatg ttgcgtggtc ttctcgattc
     1141 ggcggatatc cctttgcggg atgtgatcgt tcgactggcc gatgcggatg aggaggcggc
     1201 cgaggttgtg ctcgagggtg ggcaggggtt cacaccctgt catcgtatgg aggacatcga
     1261 agctcgactg gcggcggcgg cgcggcgagg ggcgatggat tgcagcgcgg cttgcggttt
     1321 ccgttttgac gagagcatga tcgaaatgag ctttgaccgg ctgcacaatg atgcctacgc
     1381 ggccgaccca ggcatcgagc cgatgcgggc tctgctggcc gctcgcgagg cggtcgggtt
     1441 gccgccggtc gaggtcgtcg gttggcggac gagttgcgac gcccggttgt accatgctcg
     1501 ggggtatccc gtggccatct tcggggccgg ccgtctcgat gttgctcata gcgatcacga
     1561 atacgtggat atcgccgaga tgcagcaggc cctggcgatc agcacgctgg ctgtgtggag
     1621 catgatgatg taggtcgggc ttgcccgacg cttggccggg ctgtcgttgg cgtgcagagc
     1681 acggcctact tggtcaacag gcccttctgt tgcaggtagt ccaggacctg ggtggcggcc
     1741 tcttgcgggg tcaccttgtc gttatggatg accagttcgg gcttgagcgg gggctcatag
     1801 gggtcgtcca cgccggtaaa gcccatgccc ttgccggcgg cgagtgcgtc gcgggccttc
     1861 ttgtaaaggc ccttcgggtc gcgctgttcg catttctcga tcgggacgtc gacgaagacc
     1921 tcgataaagg ccaggcctgc ctcttcgtgg attttacggg cctggtcgcg gtccttgcgg
     1981 taagggctga tgaagctggt gagtatgatg cagccggcgt cggcgaagag cttggcgacc
     2041 tcgccgatgc ggcggatgtt ctcctcgcgg tcggcggcgg agaaccccag attcttattc
     2101 aggccgtggc ggatgttgtc gccgtcggcc acgtaggcca agtggcctcg ctcgacgagg
     2161 gcgtgttcga ccgtgaaggc gatggtgctc ttgccggagc cgctgaggcc ggtaaaccag
     2221 atggtcgcgc ctttctgccc gaggagcttt tcgcggtcgg cccgactgac gtgaccttca
     2281 tgccaagtga tgtttgttgc cttgatctcc gtcatttgta tccctttgca gaacccgtcg
     2341 tttctcactc agtgttgaaa cgagtggggt agtataagaa tctgccagcc tgggctcaac
     2401 cggtcggcag gagtaggatg tcgccttgtc gcgggaaggg aatccggcca taattcccgg
     2461 ccatgagtga agcatcccga ttgaagattg tggtgaccga gcgggccagc gaggcggcga
     2521 tggcccggct ggcggccgtc ggcgacgtcg ttgtgcttcc caaatgcgat gaagccagtc
     2581 tgattgaggc ggctgcaacg gcggacgcaa tggttgtgcg gacgtactcg ttgatcaacg
     2641 cgaaggtgat cgaggcggca aaggcgggtg gacggctgaa ggtcattggt cggggcggag
     2701 tgggtctgga caatatcgac ctgaaggcgg cgctggatgc cggcatcaag gtggtttaca
     2761 cgccagccgc ttcgacgcac gcggttgccg atctggtggt ggggctgatt attgcgctgc
     2821 agcggcgggt ggtggagaac gataagggga tgcggggcgg cgagttcgat tccatccggt
     2881 cagtcaggcc cagggccaag gagcttcgcc accagacggt gggcgtgatc ggcatgggtc
     2941 ggattgggac ggccgtgggt tttcgactgg ccaacggttt cgggactcgg atcatctatc
     3001 atgacatccg cgagatcgga tggctgccgt ttccggccga gagcaagagc agccacgagg
     3061 cggtgtatgc tgaggccgac gtggttaccc tgcacgtgcc gctcacgaag ttgacgcgag
     3121 gaatgattga tgctgatgca ctggcgtcgt tcaagcaggg ctcatacctg gtcaacgctg
     3181 ctcgcgggcc ggttgtggtg gcaagggcgc tggccgaagc tttggaatcg gggcatctgg
     3241 ccggagcggc ggtcgacgtg cacgatcctg agccgccgcc ggcggattac ccgcttctgg
     3301 cagcgcccaa ttgcgtgttg accgcgcaca ttggagcccg cagccccgag ggcttggcgg
     3361 ccatggacga cgtggtcgac gatgtcattg cggttctgca gggcaaggag ccgaactggc
     3421 ccgccggccc agagttgatt gagtaaggcg cggtcggagc caccaaagga ggcggagacc
     3481 tggcgtcccg gctgaataat gcggcaggcc tcggtcgctg cggcgacctc gacgccgccg
     3541 taccgaccgg gccggagtgg atcgggtgag atgcgcgtcg ggtgcgatgc tcacggcttt
     3601 gcgtgtgcat gttcgctcga atcggtcgat attcgtagga gcggcttgcg gccgtgaggc
     3661 ttgatgagcc ggggctcact gccctgctgt ttcatggaag accggcggca taaagccgcc
     3721 ggctcgtcaa gcgaaggatg gtttttgggc attgggatgt gattctatgt caacatatct
     3781 tgtcaccggc ggcgggggct tcatcggatc gcacatcgtt catcggctgg cggccgacgg
     3841 gcagagggtc cgcgttctgg acaacttctc gaccggcaag cgggccaacc tggccgcggt
     3901 gagcaaggat atcgagatta tcgagggcga tcttcgatcg atggacgatt gtcgcaaggc
     3961 ctgcgaaggc gttgagatca tctttcacga gggggccttg ccgtcggtgc ccaagtcggt
     4021 ggccgacccg cggtcctcgc acgactgcaa catcaacggc acgttcaata ttctgatggc
     4081 ggcccgtgat tgcggctgcc gccgggtgat ctacgccgcc agcagcagcg cctacggcga
     4141 ccaggtggag tctcccaagc gtgaatcgat gcggccggcc ccgctgagcc cgtatgcggt
     4201 caataagctg gtgggggagt actacctgaa ggcctttcat ttgtgttacg gcctggagac
     4261 gatctcgctg cggtatttca acgtgttcgg cccgcggcag gatcccaaga gccagtacgc
     4321 agcggccatt ccggcgtttg tgacggcgat tctcaatgac aagccgccga cgatccatgg
     4381 tgacggcgag cagacccgcg atttcacgta catcgacaac gtggttcacg cgaacatgct
     4441 ggcggctcag gctcccaggc tcagtggcga ggtgatcaac attgcctgtg ccgagagcgt
     4501 gagcgtcaac cggacgatcc ggctgatcaa cgagttgctc ggcaaggatg tcaagccgat
     4561 ctacaccgat gttcgtccgg gcgacgtgaa gcactcgctg gccgacatca gcctggcccg
     4621 caagatcatc ggcttcgagc cggtggtgaa gttcgacgag gggttgcggc gggcgattga
     4681 gtggtatcgg gggaacctgt gaagagaagg atgaaggctg aaggatgaat cgagacagaa
     4741 gccattagcg gcgattcggc attcatcgct tgtcatccat catctcccgc gtcactaatc
     4801 gccgcttgct cactgccacg gtggtcggta tcgcgagagc agtctggtgc gacgtaaaac
     4861 ccgaaaagcc agtggcacgc gtcggcctga catctggctg ccgttctggc ggcgacgagg
     4921 ggtcgggctg gtttttctac tggttctggc gacggtcatc ctgtttgagc ggttcggcct
     4981 gtttggcggg cccggtgggc gcgagcagcc tgccgcgatc atcgccggca cggaccacga
     5041 ccgctacaac aatcgcactt tcacctgcat caaggtcatc gacggcgaca cgttatatat
     5101 tgatgccccc gacggcaaca agtcgtatac gtccgttcga ctgatcggcg tggacacacc
     5161 cgaggtcgat gagagccttg ggcggccgac ttatttcggg gctgaggcct cggcgttcac
     5221 ccgctcgcag gcggagggca aaccggtcag aatcgtgttg aaggaaaagg agactcgcga
     5281 ccggtttcgc cggctgctgg cgtacgtgta cctaggcgac ggtacgacga tgcttaacga
     5341 ggagatcatt gcccagggct acggttacgc ttacacgacg tatccgcacc cgtggacgcc
     5401 gcggttcatc gatcttgaaa gacgggcccg caagcagaaa cggggcttgt ggaaggaagt
     5461 cacaacggaa cggatgccac agtggcggcg gaagctcgaa gagccgaggt aggacagtac
     5521 caatcggttc agcgtttccg tttccgcgaa atgaagtagg cctgccattc agcgacaacg
     5581 tggttcaggt catactgcgt gccgcactcc gggcacatgt gcgtttcggg caggtgtttc
     5641 aggcagtaac cgcagatcag gcaaaggctg ttgtcgtact ccaccgctga cctttcgatt
     5701 cggcgcacct ctgtacgcgc caggaattcc aggagcagcg ccactgccgc gagtgcacag
     5761 agagccacaa ccacgcagcc ggacgagaac gttggccatt gccccggcaa gacgatgacg
     5821 aacaaagcca aaccaccggc gaggatttcg agacttcggt acctttcaag caccctttct
     5881 atgacccacg gacccggacg gtccgtcggg ccggcgagaa tggacggttg gccccgcagg
     5941 gctgagcgtg agtttcccgt cggcgtgtgc atgtcagaga attgtgacgg catggcacat
     6001 tcgtacaatt gcgttgccgt gcagtcaagc tgatctgccg acaccggcct gtcggtaggg
     6061 ggcacttaca atagagtgtg tccggccgcg cggtcggcga ggtattccga ccatgtgcgc
     6121 ttcacctgct cgatctcgta ggggctgccg cactcggggc agcggtgctg cgccggaagc
     6181 cctttaaggt tgtacccgca gtggaggcaa aggcgacagt cattggctcc agcttcgcga
     6241 agcagaacga caatttcctt cctgctccat ggtagaatca ggaagcagat cacgaaaacg
     6301 ccgcccacga tgtagacggc gtagtgagcg tgctgcaccg ggtcgacatg aaggaaatac
     6361 agaagattca gcaagaagaa caggaagacc aataaggcgc ctgcaaccat ttgcggcctg
     6421 ttctcgagcc tcttgatcac cggcggcggc acgggcgcgc ccttgggccc ggacttcgga
     6481 aggaacatca ccttcgccga gcgcacgata aagagccgga agaagaggct gatcactcgg
     6541 ccgatgtatc tcaagcggct gggcatggtc gaatgctcgt gcttgctttg cctggtattt
     6601 tggtattgag gcaaaggcgc cgcgacggcg tcagacgcca caaccgatta tgtgcgggtt
     6661 tgcttgcatc cgcaagcagg cgggcggctt gctccaagag gcttcccgga tcggaatgca
     6721 cgtggcggag gattggcaga ttgcacggtt ggccggcagg tcatcgctga tgtccggcgg
     6781 gagcggggag agggcggcaa ctcccgcgta cttccgccac gggaagaaac cgcgttggag
     6841 cgttcgtgtt ttgacttttc cccccggatt tgttcagatg atgtgcggac tcgtcttcgg
     6901 tattgcgcgt ccagagagtg tttgtcccgg gccgattgac aagtctgacg tggtttcttg
     6961 cgcgttcgta tccggagcat gggcaggctc atggcacata ccggcttggt actctcggaa
     7021 cggtacgaag atcatgacac cggtccgcac catccggagg cgcctgagcg ggtgcggacc
     7081 attcgcttgc ggatgcggga aagcggtctg gcggatgcgg ctttgctcat cgaccccagg
     7141 ccggccgacc cttcggtgat cgatcccgtt catcctcccg aatacgtgtc caggctccgg
     7201 cacgcctgcg agatgcgggc gggctttatc gacacgcccg agtgtcccgt ctgcgacgaa
     7261 acgtatgaga ttgcgctgat ggccgtgggg ggattgctgc aggcggtcga cgcggtgatg
     7321 gagggacgta ttcgcaatgc tttctgcgcg gttcggccgc cgggtcatca cgcggagcgg
     7381 cgccgggcga tgggtttctg ctatctcaat aacatcgcga ttgccgcgga gtacttgcga
     7441 tctcatcatg gtttgtcgcg cgtggctatc gtcgattttg acgtgcacca cggcaacggg
     7501 acgcagcacc agtttgagcg cgacccgaac gtgttcttct gcagcattca ccaggatccg
     7561 aatacgcttt atccgggatc gggctttgaa tgggaaaggg gacagggcga cgccgtcggc
     7621 acgacgctca acgttccgat ggccgcgggc agcggcgatg cggagtacaa acgggctttt
     7681 gaggaggtga ttcttccggc ggtcgctgat ttcaagccgg agttcatcct ggtttcggct
     7741 ggttttgacg gtcatcgggg ggatccgctc gcatatattc agctcagcac cgcatgctat
     7801 cactggatga cgcaccgcct ggtcgagttg gcgggcgagg ttgcgcgaca tcgcctggtc
     7861 agcaccctgg aggggggcta caacctggat gcgctggccg actcgacgca ggcgcatgtt
     7921 gaagcgttga tgaatgggtg aacacttccc gggatcggcg cgatttgatg caaagcgcgc
     7981 tgggatttcg gcggtagccg gctctggcgg cgatcactga cgggcgattg ccgagagcct
     8041 cgttcaggcc accacatcga cgccgccgcc ggcgggacct gagacaggcg cggccgctcc
     8101 ggccgatccg ccgccggcgt agacgtccac cgccagttgc tgcgacacgg cgaccatttg
     8161 ggtgctttga aggctgaact gggaggatga gtatatgaag gtgccgccgg cctgcgaggc
     8221 ctgttgttgc tggaggaggc tcaacatgag gccgagtaga cccttcttct catcgtcgtc
     8281 gtcgctttgc atgtattcga gcatcagaag gaggagcatc gccccaagaa tctgattatc
     8341 cgcgagcacg ggaccgtagg tgctgacgaa ggactcgacg ccggcggata tggatgtctg
     8401 cgagatggcg acggccgact ggccggccga gacggacgct ccagcgatgg gcccgccggc
     8461 cttggctgct tggccgccgt caacgccgcc caactgggct ggttgtgtgc actgcggcgg
     8521 ctgagtgggc agacttccgc cgtaaccact gattggtgaa accgcgctca tcgttcctcc
     8581 tccgggactg gcgctctccg catttccccc gcttggaggg gcatggggca gacggcgtac
     8641 cggcccgaca ggggggaaac aactatgccg taggtacgtg taagtattgc actgtcaaaa
     8701 ggttgcgaag ggcgatcggg ccgtagggtt tatgtcgttt atgacgatgc gcttgtgttg
     8761 ggggctgttc acaggcgctt gagacactgc aacatgccga ggagcatgtg gttagcgcgg
     8821 ggaggattgc acaacgcggc gaagaggaga atgacggcgg tacgcgtgct tgccggtatg
     8881 ctgcggtttc gagatgatca ggtcggccct gcggcgggcg ttggcaggac ggacgaggag
     8941 gcatagcggg ggtggcaagg atgaccgacg ggcgcgagca agggaatcgg ggcagcctca
     9001 cggtcgtcgt gggctgcatg ttcagcggca agagcgagtt tctggtttcg cagctcgagg
     9061 aagctgcccg acggggccgg gcgaccgccg ccttcaagca cagttccgat aaccgctact
     9121 gtcatcgcga cattgcgtcg catacaggcc gacgcttcag ggcgatggcc gtgccggagg
     9181 cgtcgcgaat tgcagatctg gccaaggtgg ccgaggtggt ggcgatcgac gaggggcagt
     9241 tttttgccga agatctggtc gacgtctgcc gagagctggt cgtcgccggc aaggaggttc
     9301 ttgtggcggg gctggatctg gactcgtggg gcctgccgtt cggaccgatg ccagggcttg
     9361 caggaacagc cgatcgcgtc attcgtcttc aggcggtctg cgcaagatgt ggcaggcccg
     9421 cggatcacac tcagcgagta gccccgatca cgggccagaa aatggttggc ggcgctgact
     9481 gctatgagcc gcggtgcgcc gcttgttttg aggctccgcc catcgaaaag cgatgttgac
     9541 atcacggttc agttgaaggc aacgttttga ttgagaggct cggggcggaa cggccagagt
     9601 gtccggcgag ccaggcggat acgttgccgg acaacgcgga atcgccgtgg tgagtgacgg
     9661 cggcgttgat cgcccccggt acccatcttc tcggatcagc cgattgccac acgaggctgc
     9721 cgaacgacgc cggagtcggg cgtcgccggt tcggacgatg gtcttggatg ctggctggtt
     9781 ctttgccgag cgatctgctc ctcgatgctt gtgatgatgt ctcggaggcg ctcctctttg
     9841 ataccggcag gcgtttgtcg ggccacgatc tcatccgccg ccagtagtcg ctcgatcttt
     9901 cgattggcca ggatcacagt cgagtggttc ttgtcgccca tgaagcgccc gatttcgggg
     9961 aaactcatgc gggtgtgctt gcgggcgaga tacatggcga tgttgcgagc cagggcaagg
    10021 gtgcgcgtct tgcgcgaggt gtgaaggtcg gcgggcgtca ggccgaagta cgtggccacg
    10081 atgtggtcta tgtcatcgag gccgagggct cgttgcgtgc gggccaaatg gtcggcgagc
    10141 gcgtgccggg caagggaaag ggtaatcggt tgatgtgcca cgcgactgaa catgatcagc
    10201 ttgagcaggg cgccttcgag ttcccggacg ttggcttgaa gatgttcggc gatgtatgcg
    10261 atgacttcct gcggaacggg ttgtgaggtc gcgacgcggg cgcggcggtt gaggatttcg
    10321 cagcgcatgc ggtaatccgg cacgtcgatc cgcacgacca tccccgcgac gaagcgattg
    10381 acgagttgct cgctgaattg cccgatcagc ttgggatgcg agtcgctggc caagacgacc
    10441 tgtttgcgat gcccgtcgat ggcgttgaag gtgtggagga actcctcctg agtcgccttt
    10501 ttgttggcga ggaagtggac gtcatcgatg atcaggacgt ccacgttgcg gtactgctgc
    10561 cggaaggcct cgatccgctt ggtctgcagc gcatgaatga agccgttggt gaagtcatct
    10621 ccggttacgc agagccaccg gcaatccgga tgatgctcag ccaagtggtt ggctaacgca
    10681 tgcatcaagt gggtcttgcc cacaccgcag ccgccatgga caaagacggg gctgtatgcg
    10741 gccgggtcgc gatcggcgat ccgtttgacc acggagaaag ccagttcatt tgacggaccg
    10801 acgacgagag agtcgaaacg gccgcgcagg cgggacaagg gggtagttgg ttcgtgacga
    10861 tggcggcgag ccacgcggtc ggggttcttg gcgatgaagt cggcctgtga gttgagttgt
    10921 cgctttctga gggcgtgcaa caggttcggg tcgacgacga aagaaagctg caagtcctgg
    10981 ccggtggccc ggcgggccgc gtcgctgatg gtgtccgcga aatgtctttc aatccagcca
    11041 catatgaacg cgttgggagt gctgattttc aaatgaccgt cggtcatggt gaactgcgtg
    11101 gcattcttga accacacctt gtaacgctga ggaccgacct cctgggcgat cgtgctttga
    11161 atcaaagcaa cgctgtccat gcgcggagta atcactggcg actccttcct tgcttgagag
    11221 agtagagcga ggttaccgtc tcaactgtcg ctgatgttgg cgacgatcca cggcgcctca
    11281 ggatcgtcgc ggaattgaaa ctcagcgggg cgtaacttat acagcgttct tcacgaaatc
    11341 aatacggaaa aatgccccac gcaaaggccg gtgcgccaag tgttcggtca taccgagact
    11401 tgcgacgaga aaaaaactta tccacgcgtt tggggcacag aatgtggaaa agccgtgggt
    11461 agttgcataa ctcgcgttgg tttatgaacc agcgtttttc agcggtctgg gggtttgttg
    11521 gcgggactga ggattcgcac ccatgcggat cgtcgcgatg tttccacaaa ggcaaatcgc
    11581 gcatacagcc gtcgggccgg cgaattctcg gcatccacag ccagcgtcaa tctgagggcc
    11641 gcgcgattgc gtgcggttcg caccgcatgc tcgaggagcg tggacgcgta acctttgccg
    11701 cgatagccgg gtcgcaatcc catatacacg acctcaagag cggcctgctc cggcacttca
    11761 gagagcagga gagtaccgac cggcctgccg tctgcaacac cgacgaacca agtgcgagga
    11821 ttgaacacgc cggtgcagcg gtgagtggcc atgacgtcct caatgtctct ttttccgctg
    11881 atacctgcgc aatccaggct gccttcgtaa gtgtcttgaa cggtttgcgc gaaaagctgg
    11941 tgggtttcgg gagaataggt aatccaagac agggcagggg acggcttttc gacgacgagc
    12001 ggttcggtgg actcgcgctc catgtagatc agctcagcaa gacgttgaaa ccccgcttgt
    12061 tcatacgcgt ttcgctcaag ggcagcgtcg agcggaatga tggcttgcag gaaagaaatg
    12121 ccgcggttgc gtgcctcatc agcagctttg cgcagaagac tgacaagcgt ttcagcggct
    12181 tggggatcgg gcagccaacc agggaggtag accgccgacg atcggccggg cgaatcgatg
    12241 cacaagcagc tatagaggat attcctttcg tggcgtgcga tcaggcagtg tttgagcgac
    12301 aggccgcgtc tttcggcgtg gttgaccagt gttttgaccc gacggtcgat ctctgccggg
    12361 gacaacttcg gatggtaaac gagccgctga agcccgcgct gaagctcacg atctgttgcg
    12421 ggtgtggaga tgaccataaa cctgttagcc tccgccgccc gagagcttac cgaaaattga
    12481 cgaggcggcc aacgggcctt gacggggtcg gcgggtcctc agcgaggcgg gtttgcggat
    12541 aagaaggtgc cccgcggcaa gcgcgaggag tagccttcca cgtccaagtc gcatcgcggg
    12601 acgacgacaa cagtccaggg gcttgccagt cgaaacccgg gtctgatgcg cgaaccaggg
    12661 tagatttgcc gttcgagaac gcggagtctg tcaccctctt tccagacatg ctggatgatg
    12721 atcccgcagt catctcgcgg cgtggggcca agaccggcaa tcagaatcat ctcggttcgg
    12781 aaatcaacgg gaacctcgca caagggtatc tgggcgagtg tggccggatc acgaacaacc
    12841 aaccgaactg cctttgtgag gcgggaatac gtccctgatg cctgccagag gatgggtaat
    12901 tcctcggtgc ctgccgggac ggtggtagga cttgccgtgt cgaggcgaca acctaacagc
    12961 gtcgtcgcac caacaagcgc tgcaatcatg ccatgcgatg tcgaatggga atgaaagttt
    13021 cttcgcatgg catgcgatca taccatggtt gcggcagagt ttcgcggtcg aacggtgagg
    13081 atcggccatg gcggttttct gtctccagcg agggaaccgc ctgatgatgg cagcccaatc
    13141 ctctggtttt ggctttgctg gtcgcggccc gccatcgaac aggcgactcg gagcacgacg
    13201 tggtcacgtc ggtcgcggac ggccgaattg ggctgcaaag gcgtcgaaat cgtcctgcag
    13261 aatctcgggg atctggacca agccggcggt caccgcgctt gcgaggtctg ccaggtcttc
    13321 gaaagtattc acgccattcg ctgcaaggaa ctgttcgaga gcctcggctt cctggtcgtc
    13381 caagtcaggg agggctatgt cgggcggcaa gtagaagccg agcttggcca actcgtcggt
    13441 aagcgtgggc agttgagtga caagaatctg caattcggct gagttgaggg cgccgatgtt
    13501 cttcacggga tcggtcagct tggtcagcgt tgacagcact gttccgcgat ccccattgct
    13561 gttgacggtg ggtggcgagc aaccggctaa cggcaagaac atcatggcac cgatgcagga
    13621 accgaccaga cgcgtgcggg atctcattgc actctccttg tcgattatcg gcccagggca
    13681 aatgccttgg cgccgggggt tggatcggtt ctcgaagcat gcgtgtttag tcccgctcgt
    13741 gtttgtccgc ctggtgaccg tcccgcggag ttgtcattcc ggcctgacgg gaggatgacg
    13801 gcgttttccg aggcggggga ccgccgcagc gataagacat taagtggggc cgataagcgc
    13861 ggacggccgt cttttcccag gtgccggcag ggcctatagt gtccatgagg ttaacccgag
    13921 aatcgcccgg cctttgcctt gggatcgaga tcatgatgga cgaccgacaa ctggatatcg
    13981 tttttgccgc acctcacccc gacgacctgg aaatcggctg tggaggcacg attgcttcgc
    14041 tggcggacaa aggctatcag gtgggaatgg ttcatttgac caatggcgag ccgacgccga
    14101 acgggtcgcc cgaacgccga tcgcaggagg ccgtgaaagc ggcagaagtg ctgggggcgg
    14161 cggtgtgcga gatcctgccg ctgacaaacc gcgaactcat ggatgggccg ccggcccgct
    14221 atgcgctggc aacaatcttg cgacgatatc gcccgcggat cctggtcggc ctggcagggc
    14281 ggacgaccgc ggcttctccc gaccactatc aggcacagct aatagttgag gcggcgcgct
    14341 tctactcaca gctcaccaag tgggatgacc gttttggcca cacatccccg caccggatcg
    14401 atcatctcgt ctatcgcccg atcccgtcgg ccgcagagcg gatgcacttt catgggcagt
    14461 tcgtggtgga tatcacggac acactggaac gtaagcttga ggcgattcgt tgttaccgtt
    14521 cgcagttcga cggccagcga tttgatcgac tccgacatta cgtaaccagc gtggcaggcg
    14581 cggagggcgg ccaagccggc taccggtacg gggagctgta ctgtctgcct cgcccggtgg
    14641 gcgtcaagga catgattgcc atgctgggtt cgtgggagat tccgccgccg ttctccccat
    14701 attccagtgg gtaaccatct ggcgcgggtt ttccgcccgg tccggataga ggcggaagaa
    14761 cctcggttgc ggccggcggg cttagccgtt gt
//
LOCUS       DAOTPW010000139        14790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_3970_length_14790_cov_5.344350, whole genome shotgun sequence.
ACCESSION   DAOTPW010000139 DAOTPW010000000
VERSION     DAOTPW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14790
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_14790_cov_5.344350"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="P5316_13205"
     CDS             complement(<1..308)
                     /locus_tag="P5316_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85961.1"
                     /translation="MRLVAVMIGAMALTGPAPANGPYLASGVKLGEVSQDSALVWVRL
                     TAEPKRRDGIVLKGLTTLPADVQVNDLQGAVPGTNGRARVRFGTKEDLSDAAETPWTA
                     V"
     gene            complement(319..1011)
                     /locus_tag="P5316_13210"
     CDS             complement(319..1011)
                     /locus_tag="P5316_13210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRR85962.1"
                     /translation="MAGPHVRKAKWLLRSLTGTQAEVIQAEFEVGRDMAGAIARSGQL
                     DADPRWQRILAQTGGRLVNRLAKRERRFSFSALADPQPNAFALPGGFVYVTRPLLEIC
                     ATKDDELAFVLAHEMAHVVRGHVMDRVLSSTLLNVASRASLAARMMNKAVLEAALKLL
                     QNAYSQEQETEADRFAVRLLLSAEMNPLAGAAVLGKLSSAGDGSDELPSYFSTHPPSS
                     ARMDRIHQWLNK"
     gene            complement(1044..3263)
                     /locus_tag="P5316_13215"
     CDS             complement(1044..3263)
                     /locus_tag="P5316_13215"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR85963.1"
                     /translation="MKLVGPASVTLLKLALTMALPGCSQGPGDLTLTNATEPPVIPVG
                     LDAFRMWDRWAYLRIGQRTYMTSTYDRCGANEAADAGHFLYQLADDRNVSLDLMGKGV
                     LAFKRTNHWHGSPWYYTVDGRDYVVQESSTADPDHPVENSVFIPREQFPHPLTWTWSI
                     TKGADLMWVPIPFEQSFTLAYGRTHYGTGYYIYHKFLPDAPNLSRPIKSWNMDVPPAD
                     VLDLINRSGTDIAAGSSDYAARTAINVPAGQTRSLFSFSGTTAKTVRAFRLTISQDQA
                     KALGEAFLRIYWDGNKHASVNAPVKLLFGTGSMFNRENREYLVKAFPVNIRFGDGQVC
                     FSMYFPMPFHRSARFALQAAPELAINDLQASIQCQNYTDPANWVGYFHATYVDHGEPK
                     PGHDLVFLDTTQVEGGGDWCGHFVGTSFIFSDRAELRTLEGDPRFYFDDSNSPQAQGT
                     GTEEWGGGGDYWGGRNMTIPFAGHPVGCRRKEEAKDPEDLIQSAYRFLLSDLMPFGRN
                     ARITFEHGGENNMPEHYRSVTFWYGINQPCLILTDTFNVGDTDDERRHDYDSPQASPV
                     QTLSSRYEWGVDHLDGKEIFPETTDKGRFTKGSSTFSLKLDPRNIGVMLRRKMDYGFP
                     NQCAKVFVADHKAGAEWREVGMWYTAGSNTVVYSNPKAETGAAEHYVQTSNRRWREDE
                     FLLPRSATEGRSVIRVKVEFVPRNIPLFPGHPPAEQAWSEYQYKAYCYVMPQAKCEG"
     gene            3404..4939
                     /locus_tag="P5316_13220"
     CDS             3404..4939
                     /locus_tag="P5316_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR85964.1"
                     /translation="MRRTVSSTGFHTICTVILVGMTATPLSRAQAKASERPNVLFIAV
                     DDLRPDLACYGVKHIHSPNIDKLARSGTVFTRAYCQQAVCSPSRTSLLTGRRPDTTKV
                     YDLQTHFRIHLPDVVALPQYFKQQGYHTQSMGKIYHGGLDDPQSWSVPSWFPKGQGYH
                     KPESLRAIEETRSRLKAEGKLYKEEVVRRDEKTGTVLKIKRSGERVRGPAWEDPDVAD
                     NELADGMVADKAIEALRQIKDKTFFLAVGFYKPHLPFIAPAKYFDLYPAAGIRLAANP
                     FPPKDVPHVALHDFGELRSYSDMPKVGPVSESKARELVRAYQAASSFTDAQIGRVLDE
                     LERLGLRDKTIVVLWGDHGWHLGEHGMWCKHSNFEVATRVAMIISAPKQKTAGKTTAA
                     LSEFVDIYPTLCELAGLPLPAGLEGTSLVPVMNDPDRPWKKAAFSQYPRQKVMGYSLR
                     TDRYRYTEWVEPGQEPVGVELYDHQVDPDENLNLARLPENKELVVKLSKMLKDGWRAA
                     KPE"
     gene            complement(5050..6189)
                     /locus_tag="P5316_13225"
     CDS             complement(5050..6189)
                     /locus_tag="P5316_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85965.1"
                     /translation="MKRLTLLLSVSMLLATPVLVLGQAKSDRPLPRNEEEYRAWFAAQ
                     PRHLYGCGIIWAQWVWPGDKTKWNYDGRSMQMVKNMGGTNVPVNIPWYDVEPKEGEWY
                     WDYVDHQVAEAENRGLPMFAYMGLTPDWALPPEAPKGMTGIGYRYPPPDDKKEQFIRY
                     CKEVAKRYKGRIKHYQFWNEPNGCSWVNDGCGNGHEAENTRKLYTKWLIIWYNAMKSE
                     DPDYVLGAGALDYHEGVAKGYEYLEGMYKHGAKDHFDAFNIHPYDSKGTLHHRAIEDT
                     RRVMVEHGDGHKGIWISEWGWSMKDEDEKSRRIVKTLRELNDPKYYYVTMANYLSITD
                     PAGEPGFGLCDRDLKPRKSYMAFRAYTREHPAKHVAPSTRPSGDK"
     gene            complement(6685..7875)
                     /locus_tag="P5316_13230"
     CDS             complement(6685..7875)
                     /locus_tag="P5316_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRR85966.1"
                     /translation="MFKQVVNLIEPIIPDLTAFRRDLHSHPELSRREYRTSEKIRATL
                     AKLPNLKVLPALTGTDVIAILNADKPGRCIAIRADIDALPIQEQTGVEYQSTVPGVMH
                     ACGHDGHTAVLLGTAMVLSKLADAIPGKVKFVFQPDEEDEGGGRRLCENGVLESPKVE
                     GIVALHAWPSQPVGTISFSRSTVTAASSPFTIRVIGVGGHGAYPHRSVDPIVISAHII
                     TALQTLVSRVVDPRDAAVVSVGQIRAGSADNVIPPEAEMAGTMRYLQPATGEQLHECF
                     KRLVEQTAAAHGGRAEIKIHQGYPPLINDSTMVALIEGAVCDLFGHDHMVIENMPSMG
                     VEDFAYYAERVPAAMFRMGIRPAHLSDYPHLHNPGFNFNDEALPVGIRMFCEIVRRFF
                     DVAD"
     gene            complement(8003..8629)
                     /gene="lexA"
                     /locus_tag="P5316_13235"
     CDS             complement(8003..8629)
                     /gene="lexA"
                     /locus_tag="P5316_13235"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRR85967.1"
                     /translation="MAQHKFARLTPKQLQILTMIRDYQHRHGYSPTLQELADTLGVSK
                     VTVFEHVTGLEKKGLLRRSRHRARSLELTEHGRAAFPDDSRYTLPLVGHIAAGSPIEA
                     VENLETVSLDDLFASRSGAFVLRVRGDSMIDEQIRDGDLVIAEQRNTARNGETVVALL
                     DNGEATLKKFYKESNRIRLQPANPAYQPIYADHVNIQGVVIGVIRTYR"
     gene            9544..10029
                     /locus_tag="P5316_13240"
     CDS             9544..10029
                     /locus_tag="P5316_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRR85968.1"
                     /translation="MLSFTRKTHYALIALTHLASQPTGCSSARQISAAYRLPLPLLMN
                     LLKQLTQQELVRSVRGPRGGYTLTLPPDKIRLTDVVRAVEGPIHVTQCVPDKAVNPEE
                     DDADTCVRMESCPIRASLELVHNKMIGYLDSITLADIIGGGKEACHGRQPVEESHGVI
                     S"
     gene            10023..11240
                     /locus_tag="P5316_13245"
     CDS             10023..11240
                     /locus_tag="P5316_13245"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRR85969.1"
                     /translation="MVKSLIYLDNNATTPVDPRVLEAMLPYFREKFGNAASRTHQFGW
                     EAEKAVDLAREQVAALINASAKEIIWTSGATESNNIAVKGVAQMYKDKGRHIITQVTE
                     HKAVLDPAKYLETQGFEVTFLPVDRTGRVDVNQFREAIRADTILVSIMMANNEIGTIQ
                     PIAEIGAVCKEKGVIFHTDATQAFGKVPIDVKAMGIDLLSCSGHKVYGPKGVGALYVR
                     RKGPRVRCEPILHGGGHERGMRSGTLNVPGIVGFGKAAEIAGQEMQAESARQARLRDR
                     LWAGLSEHLDEIYRNGHTVHCLPNTLNVSFAYVEGEALMMGFSNIAVSSGSACTSASL
                     EPSYVLKALEVADELAHSSIRFSLGRFNTAAEIEQTIDQVAATVRRLREMSPLYEMAK
                     EGVDIKGVDWSRI"
     gene            11292..11711
                     /gene="iscU"
                     /locus_tag="P5316_13250"
     CDS             11292..11711
                     /gene="iscU"
                     /locus_tag="P5316_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRR85970.1"
                     /translation="MAYSDKVIDHYNNPRNIGSLDKNNPRVGTGIVGAPECGDVMKLQ
                     IQVDDDGCIVDAKFKTFGCGSAIASSSLATEWIKGKTLDEALEIKNTDIVRELALPPV
                     KIHCSVLAEDAIRAAIQDLKRKQANAPATAGAQVARG"
     gene            11823..12155
                     /locus_tag="P5316_13255"
     CDS             11823..12155
                     /locus_tag="P5316_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020007826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRR85971.1"
                     /translation="MALALTERAAQEVKAILQQQNLSPDSVYLRLGAKGGGCSGFTYA
                     LDLVDTKGELDQEYVSHDIRVICDPKSLIYLDGTTLDFKDELMGRGFVFDNPNASGTC
                     SCGNSFCT"
     gene            12234..12869
                     /gene="hscB"
                     /locus_tag="P5316_13260"
     CDS             12234..12869
                     /gene="hscB"
                     /locus_tag="P5316_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HRR85972.1"
                     /translation="MSSQPTTSRPIRCTTCSQPLESPICCTSCGQLNADPIGRCSYFE
                     LFGLPVDFDIDEQSLHRTYLSLTRSVHPDVAGHASQEKRHDALAISSEMNRAYLTLRD
                     PVLRAEYLLSLVGEPGTSEDRAVSPDLLGEVMTIREEIEEARQAGDQASLEDLKQQVA
                     AKQQACMESIVSLARALVEKPDARRALRRQLNAIKYWNNLMDRLLPGSDGV"
     gene            12880..>14790
                     /locus_tag="P5316_13265"
     CDS             12880..>14790
                     /locus_tag="P5316_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRR85973.1"
                     /translation="MSDNRDVIVGIDLGTTNSLVAVFDEAGPRVIRDEMGESRVPSVL
                     AFSAEGRVTIGWEAKAHAVENPTSTVFSIKRLMGRGYEELAASGELGHLPYQVVKRVT
                     DSSSRDIAAVNVNGRLMTPPEISALILRELKRRAEAHLGRPVSRAVITVPAYFDDAQR
                     QATRDAGRIAGLEVVRIVNEPTAAALAYGLGLRGQMDRIKAAGSMLTLAGQCPTSRDG
                     ADAEAQGRCSKPGGGESLIAVYDLGGGTFDISILRLMDGVFEVQSTHGNTRLGGDDFD
                     REIIGLVQREVGEQFGLEINSPAIRQALRNFAESVKMRLSCDETATIEIDLGQGRRYR
                     RSISRSEFEAMIEPWIAKTLESCRRALADAKVKAAEVDQVILVGGSTRIPLVRRRVEE
                     LFGRRPYTALNPDEVVALGAAVQAAILAGEQRDALLLDVIPLSLGIETMGGAMGKLIL
                     RNTRIPCQATERFSTFVDGQTNVKINVLQGEREMAKDCRSLGEFELRGIPPMPAGLPK
                     ILVTFLIDENGILNVSAREERSGTEASIQIVPTYGLTSDEVKRMEMESYEHAREDMTA
                     HRLIDLRNQIEFDTHKTEQMLAKVGDRLLPDERQKIETAMAELKALAAANGTDADALH
                     KALIEFDRKTVRL"
BASE COUNT         2850 a         4365 c         4658 g         2917 t
ORIGIN      
        1 accgccgtcc acggtgtctc ggcggcgtcg gacaagtcct ccttggtgcc gaaccgcacc
       61 cgcgctcgcc cgttcgtgcc cggtaccgct ccctgcagat cgttgacctg aacgtctgcg
      121 ggcaatgtcg tcaggccttt caggacaatt ccatcgcgcc gcttgggctc ggcggtcagt
      181 cgcacccaaa ccagggccga gtcctgtgag acctctccga gcttgacgcc gctggccaga
      241 tacgggccgt tcgccggcgc cggcccggtc aatgccattg ctccgatcat caccgcgacc
      301 aatcgcatgt tctctacctc atttgttaag ccattggtga atcctgtcca tccgcgcgct
      361 cgacggcggg tgcgtcgaga agtagctcgg aagctcgtcc gagccgtcac cggcgctgct
      421 caatttgccc aggaccgcgg caccggccag cgggttcatt tcggcgctga gcaatagccg
      481 cactgcgaag cggtcggcct ctgtttcctg ctcttgcgaa taggcgtttt gaagaagctt
      541 cagggccgcc tccagaacgg ccttgttcat catccgggcc gccaggcttg cccgcgacgc
      601 tacgttgagc agcgtgctgc tgagcacgcg atccatgaca tggccgcgga caacgtgggc
      661 catctcatgg gccagcacga aagccagttc gtcgtctttc gtcgcgcaga tctccagcag
      721 cggccgggtg acataaacaa aaccgccggg taaagcaaag gcattcggtt ggggatctgc
      781 caatgcgctg aaagagaacc ggcgctcccg tttggcgagt cggttgacca gccggccacc
      841 tgtttgagcc agaatccgct gccagcgtgg atcagcgtca agttgccccg accgggcaat
      901 ggcgccggcc atgtcccgcc cgacctcgaa ttccgcctgg atcacctcgg cttgcgttcc
      961 ggtcaacgag cgcagcagcc acttggcctt gcgaacgtgc gggcctgcca ttcgccccag
     1021 atttcgaaag agaccggcca acgtcagccc tcacatttcg cctgcggcat gacatagcag
     1081 tacgctttgt actgatactc gctccaggcc tgttcggccg gcgggtgtcc agggaacagc
     1141 ggaatattgc ggggaacgaa ctctactttc acgcggatga ccgagcggcc ctcggtggcg
     1201 gagcgcggca gcaggaactc atcctcccgc cagcggcgat tcgaggtctg cacgtagtgc
     1261 tcggccgctc ccgtctcggc cttgggatta ctgtagacca cggtgttcga cccggccgta
     1321 taccacatgc cgacctcgcg ccactcggcg ccggccttgt ggtcggccac gaagaccttg
     1381 gcacattggt tcgggaagcc gtagtccatc ttgcgacgga gcatcacccc gatattccgc
     1441 gggtccagtt tgagcgagaa cgtcgaacta cccttggtga agcggccttt gtcggtggtc
     1501 tcgggaaaga tctccttgcc atccaggtga tcgacgcccc actcatagcg cgagctgagc
     1561 gtctgcaccg gcgaggcctg cggcgaatcg tagtcgtggc gccgttcgtc gtccgtgtcg
     1621 ccgacgttga aggtgtccgt gagaatcaag cagggctggt tgattccgta ccagaaggtt
     1681 accgagcgat agtgctcggg catgttgttt tcgccgccgt gctcgaaggt gatcctggcg
     1741 ttcctgccga agggcatcag gtcgctcagc aagaaccggt aggccgattg aatcaagtct
     1801 tcggggtcct tggcctcctc ctttctgcgg cagccgaccg gatgaccggc aaacgggatg
     1861 gtcatgttac gtccgcccca gtaatcgccg ccgccgcccc attcttcggt gccggtgccc
     1921 tgagcctgcg gcgagttgct gtcgtcgaag tagaagcgcg ggtcgccctc aagcgtccgc
     1981 aactcggccc ggtcggagaa aatgaacgaa gtgcccacga agtgtccgca ccagtcgccg
     2041 ccgccctcga cttgggtggt gtcgaggaat accagatcgt ggccgggttt gggctcaccg
     2101 tgatcgacat acgtcgcgtg gaagtagcct acccagttgg ccgggtcggt gtagttctgg
     2161 cattggatgc ttgcctgcag gtcattgatt gccagctcgg gagcggcttg cagcgcaaac
     2221 cgtgcggagc gatgaaaggg catcgggaaa tacattgaga agcatacctg tccgtcgccg
     2281 aatcggatgt tcaccgggaa cgccttgacc aggtattccc gattttcgcg gttgaacatc
     2341 gagccggtcc cgaaaaggag cttgaccggg gcattgaccg acgcatgctt attgccgtcc
     2401 cagtagatgc gaagaaacgc ctcaccgaga gccttggcct ggtcttggct gatcgtcagt
     2461 ctgaaagccc ggacggtctt ggccgttgtc ccgctgaaac tgaaaaggct gcgggtctgg
     2521 ccggcgggca cgttgatcgc tgtcctggcc gcgtaatccg aactgcccgc ggcgatatcc
     2581 gtcccgctgc gattgatcag atcaagcacg tcggcggggg gcacgtccat gttccagctc
     2641 ttgatcggcc ggctgaggtt cggggcgtcg gggagaaact tgtgatagat gtagtagccg
     2701 gtgccgtagt gcgtccgccc gtaagccagc gtgaacgatt gctcgaaggg gatgggcacc
     2761 cacatcaggt ccgcgccctt ggtgatcgac caggtccagg tcagcgggtg ggggaactgc
     2821 tcccggggga tgaacactga gttctcgacc ggatggtcgg ggtcagcggt gctcgattcc
     2881 tgcacgacgt agtcccgccc gtccacggtg tagtaccacg ggctgccgtg ccagtggttg
     2941 gtgcgtttga aggccagaac tccctttccc atgagatcca gtgagacgtt gcggtcgtcg
     3001 gccaactggt agaggaagtg gccggcgtcg gcggcctcgt tggccccgca gcggtcgtac
     3061 gtactggtca tgtaagtccg ctggccgatt cgcagatatg cccagcggtc ccacatgcgg
     3121 aaagcgtcca ggccgacagg tatgaccggg ggctcggtcg cattcgtcag cgtcaggtcc
     3181 cccggacctt ggctgcagcc gggaagggcc attgtcaatg ccagtttcaa tagcgtcact
     3241 gaagcaggcc cgaccagctt cattgtaaac ctcccacaag cgggcaactg tgccgactgc
     3301 tttgcgagcg gcgcggataa gtgtggctta ccgccgcgcc gcatggtaca ttgctccacc
     3361 agaccaatca aactgcatat acaccggtca taaggagtgc caaatgcgta ggaccgtttc
     3421 gtcaaccgga tttcacacta tctgtaccgt gatcctggtt gggatgaccg ctactccgtt
     3481 gagccgggca caggccaagg caagcgagcg ccccaacgtg ctttttattg cggtagatga
     3541 tcttcgcccc gatcttgcct gctacggggt taagcacatt cattcaccga atatcgacaa
     3601 gctggctcgc agcggcactg tgttcacgcg ggcctactgt cagcaggcgg tgtgcagccc
     3661 ctcgcggacc tcgctgttga ccgggcgacg gccggacacg accaaggtgt acgacctgca
     3721 aacgcatttc cgaatccatc tgcccgacgt ggtcgcgctc ccgcagtact tcaagcagca
     3781 gggataccac acgcagtcga tgggcaaaat ctaccacggc ggcctcgacg acccgcagtc
     3841 ctggagcgtg ccgtcgtggt ttccaaaggg gcaaggctat cacaagccgg aatcgctcag
     3901 ggccatcgaa gagacacggt cacgtctgaa agcggagggc aagctataca aagaagaagt
     3961 cgtccggcga gacgaaaaga cgggaaccgt cctgaagatc aagcgaagcg gggaacgagt
     4021 ccgtggtccg gcctgggagg atcccgacgt cgcggacaac gaactggctg acggaatggt
     4081 tgcggacaaa gctatcgaag cgctgcgtca gatcaaggac aagactttct tcctggccgt
     4141 gggcttctac aagccgcatc tgccgttcat cgcccccgcg aagtacttcg acctgtaccc
     4201 ggccgcgggt atccggttgg cggccaaccc tttccctccg aaggacgtgc cacatgtggc
     4261 gctccatgat ttcggcgagc tgcgttccta cagcgatatg cccaaggtcg ggccggtgtc
     4321 ggagtcaaaa gctcgcgaac tggtacgcgc ctaccaggcg gccagcagct tcacggatgc
     4381 tcagatcggg cgggtgctcg atgaactcga gcggctcggg ctgcgcgaca aaacgattgt
     4441 tgtgctgtgg ggcgatcatg gctggcacct cggcgagcac ggcatgtggt gcaagcacag
     4501 caacttcgag gtggccacgc gcgtggcgat gattatctcc gcgccgaagc agaagacggc
     4561 cggcaagacg accgccgccc tgtcagagtt cgtggacatc tatcccacgc tgtgcgaact
     4621 ggcggggctg cccctgcccg cgggcttgga gggaaccagc cttgtgccgg tgatgaacga
     4681 tcccgatcgc ccgtggaaga aggccgcctt cagccaatac ccgcggcaga aagtcatggg
     4741 ttactcgctc cggaccgacc gctaccgcta caccgagtgg gtcgagccgg gccaggagcc
     4801 ggtcggcgtc gaactgtacg accaccaagt cgacccggac gagaacttga acctggcgag
     4861 gcttcctgag aacaaggaac tcgtggtcaa gctgagcaag atgctcaagg acggttggcg
     4921 agcggcgaag ccggaatagg ggattggtct gttagtctat tagttgggta gtctgctgga
     4981 tgggagccga gagaggcgcc tcgctcggtc cgactgacaa ctgattactg aagactgaca
     5041 actggttttc tatttgtccc cgctcgggcg agttgacgga gcaacgtgct ttgcagggtg
     5101 ctcccgcgtg tatgccctga acgccatgta cgacttgcga ggtttgaggt cgcggtcgca
     5161 cagtccgaaa cccggttcgc cggccgggtc ggtgatcgac aggtagttgg ccatggtgac
     5221 atagtagtac ttcgggtcat tcagctcgcg gagagtctta acgatacgac ggcttttctc
     5281 gtcttcgtcc ttcatgctcc aaccccactc gctgatccag atgcccttgt ggccgtcgcc
     5341 gtgctcgacc atgacgcggc gggtgtcctc gatggccctg tggtgcaacg tgcccttgct
     5401 gtcgtaagga tgaatgttaa aggcgtcgaa gtgatccttc gctccgtgtt tgtacatccc
     5461 ttcgaggtac tcatacccct tggctacgcc ctcatggtag tcgagcgcgc cggcacccag
     5521 aacgtaatct ggatcctcgc ttttcattgc gttgtaccag attatcagcc atttggtgta
     5581 gagtttgcgg gtgttctcgg cctcgtgtcc gttgccgcag ccgtcgttga cccaactgca
     5641 tccgttgggc tcgttccaga actggtagtg cttgatgcgg cccttgtacc gcttcgccac
     5701 ctccttgcag tagcggatga actgctcctt cttgtcatcc ggcggcgggt agcggtagcc
     5761 gatgccggtc atgcccttgg gagcttccgg cggcagggcc cagtccggcg tcaggcccat
     5821 gtatgcgaac atgggcagcc cgcggttctc ggcctcggcc acctggtgat cgacgtagtc
     5881 ccaataccac tcgccttcct tgggctcgac gtcataccag ggaatattga cgggcacgtt
     5941 cgtcccgccc atgttcttga ccatctgcat cgagcggccg tcgtagttcc atttggtctt
     6001 gtcgcccggc cagacccact gggcccagat gatcccgcag ccgtacaggt ggcggggctg
     6061 cgcggcgaac catgccctgt attcctcctc gttcctgggc agcgggcggt cgctcttggc
     6121 ctgccccagc accagcacgg gtgtcgcaag caacatcgat acagacaaca acagagtcag
     6181 ccgtttcatg accatttcct gcgcggtcga gtcccgctgt cttcacctgg atgcacagat
     6241 gcacatcgtc tccgcagacg gggaacatac taccgtgcag gatgcgatcg gtgcaatcgg
     6301 ccggaggtcc gtcgcctgcg gcggggtgca tccctgaaac ggtggcacat ggattggcgg
     6361 ccgccacggc aggccagtgt cgaggccgcc accgggaccg agacctgccg agaacgacca
     6421 ggatcgtcgg cgggtctcat ccgctgcgcg gatttcgacc atgccctgcc ttcgaaacgc
     6481 caccgaagac ggaatacacc ccctgcagca tcggatctga aatctgtaat ctcagatctg
     6541 aaatcctcga tctgagattt gagatcgggc gatctggaat ccgcgacgcg gagcctgggc
     6601 ctcacattcg gtgggacata taattgaata cgaaggatca tatagaaagt ttatggccag
     6661 atcatgcgag ggcgtcgagt cagctcaatc cgccacgtcg aagaaacgcc gcacgatttc
     6721 gcagaacatc cggatgccga cgggcagggc ctcatcattg aagttgaagc ctggattgtg
     6781 caggtgcggg tagtcgctca ggtgggccgg gcgaatcccc atccggaaca tcgccgccgg
     6841 cactcgttcg gcgtagtacg caaagtcttc cacccccatg ctcggcatgt tctcaatgac
     6901 catgtgatcg tggccgaaca aatcgcagac cgcaccctcg atgagggcca ccatcgtcga
     6961 gtcgttgatc aacggcggat atccctgatg gatcttaatt tcggcgcgtc cgccgtgtgc
     7021 ggccgcggtc tgctcgacga gccgcttgaa gcattcgtgg agctgctcgc cggtcgccgg
     7081 ctggagatag cgcatggtcc cggccatctc agcttcgggc ggaatcacgt tgtcggccga
     7141 accggcgcgg atttgtccta ccgatacgac ggctgcatcg cgggggtcga cgacgcggga
     7201 cacgagagtc tgtaatgccg tgatgatgtg tgccgagatg acgatcgggt cgacgctacg
     7261 atgcgggtag gcaccgtgtc cgccgacgcc gatcactctg atcgtaaacg ggctgcttgc
     7321 ggccgtgacc gtgcttcgcg agaaggagat cgtgcccacg ggttgtgagg gccacgcgtg
     7381 cagagccacg attccttcaa ccttcggcga ttcaaggacc ccgttctcgc acaagcggcg
     7441 acctccgccc tcatcttctt catccggctg gaaaacgaac ttcaccttgc ccggaatggc
     7501 atcggcgagc ttcgacagaa ccatcgcggt gccgagcagc acggccgtat gtccgtcgtg
     7561 cccgcatgcg tgcatcacgc ccggcaccgt agactggtac tcgacgcctg tctgctcctg
     7621 aatcggcagc gcatcgatgt ctgcacgaat agcgatgcat cgacccggtt tgtccgcgtt
     7681 caatatcgcg atcacgtccg tgccggtcag cgcgggcagc accttgagat tgggcagttt
     7741 tgcaagggtc gcccgtattt tttcgctggt gcgatactcg cggcgtgaca gctccggatg
     7801 cgaatgcaaa tcgcgacgaa aggccgtgag atccggaatg atgggttcga tcaggttgac
     7861 gacttgtttg aacattggcg atgccgctcc tgccagtgcg tgatcacgac gtcgacgccg
     7921 gcatgacggt taaccggcat cgcaagtcga tccggatcct ccttggggcg gcccgtggtt
     7981 gcattcggtt ccgggccgaa gcctaacgat aagtgcggat cacgccgatg acaactcctt
     8041 gaatgttgac gtgatcggcg tagatcggct gataggccgg attggccggt tgcaggcgga
     8101 tgcggttact ttccttgtag aacttcttga gcgtggcttc cccgttgtcg agcaaggcta
     8161 ccactgtctc accgttgcga gccgtgtttc gttgctcggc aatgaccaag tcgccgtcgc
     8221 ggatttgttc atcgatcatg ctgtcgcccc ggacgcgcag cacaaacgcg ccgctcctgc
     8281 tggcgaacag atcgtcgagg ctgacggtct caagattctc caccgcttct atgggcgagc
     8341 cggccgcgat atggcctacc agcggcagcg tgtaccgcga gtcatccgga aacgctgccc
     8401 gcccatgctc cgtaagttcc aaagagcgtg ccctgtgtct cgatcggcgc aggagacctt
     8461 ttttttcgag tccggtgacg tgttcgaaga cggtgacctt gctgacgccc aacgtatcgg
     8521 ccagttcctg caaagtcggg gaatagccat gcctgtgttg atagtcgcgg atcatggtta
     8581 gaatctgcaa ctgcttgggc gtcagacgag caaacttgtg ctgtgccata ttcaggctcc
     8641 gtgagttgcg gttgcgccag ttccaagggt ccgatgacca caagcggcca cgccgccagc
     8701 ctgcgtggtt catccatttc tccggccgtg ttccactgaa tcgtcccggc attcctggtc
     8761 gccaagtcga accctgccag cgccaactgg cgaccggtca ttcggcctgg ctcgccgatt
     8821 actccctcat gttgctcggc cggtgctcct cccgaagtcc ggcgtttgcc gaacgttgcc
     8881 gtcacatccg agccttcctt cggatagcaa gagacacgtt ccagcagcgc tgacagcttc
     8941 tgtcccaacc aaccggctgg gaaatgggtt cctttgatgc ccggccgaac cggccaagcg
     9001 gtacggaatc tcccgccggg cccgaacacg catactggca gttcaccacc ttgagaattc
     9061 gccatgccag ccgcctcctt acggcacggc caaccatcct aatcatgagg agcctagtct
     9121 cacggccaag tcgagtatcc tgcctcgggc cagtcgccta tctggcggac gatgagctag
     9181 atgctgttta ctttttgttt gggaatagaa taccctaacc atctccaaac gtcaaatgtt
     9241 ttttcatttt tctttttcgg aagtcctcca gggccttggt cttcaggcca gcccccaaca
     9301 tatgctcccc gccgtagtcc ggaaccccac ttaatggaca tatcgtgtcg ttcatagcca
     9361 aaacgtgaac ggcctattgt cccttttttg ccggcgaccg ggcctgaaaa tgcgcgtttt
     9421 tccggctctg cggggaggca aggagcagaa cgtccggctt caacgcaacg gccggcttga
     9481 aatcggactg attgggtgct aaaagaaggg cgtacaagat gactatgtcc gggggctgag
     9541 acaatgcttt cattcactcg caagacgcat tatgcgctta tcgcgctgac ccatttagca
     9601 tcgcagccga caggatgcag cagtgccagg caaatctcgg cggcttaccg tttgcccctg
     9661 cccctgctga tgaacctgtt gaagcagttg acgcagcagg aacttgttcg atcggtcagg
     9721 ggtccgcgag ggggatatac gctcacgctg cctcccgata agatccggtt gacggacgtg
     9781 gtcagggccg tggagggtcc gattcatgtg acgcagtgcg ttcctgacaa ggcggtgaac
     9841 ccggaagaag acgacgcaga tacgtgcgtt cgaatggaga gctgtccgat tcgggcatcg
     9901 ttggagctgg ttcacaacaa gatgataggg tatctcgact caatcacctt ggccgacata
     9961 atcggcggag ggaaagaggc gtgtcatggc cgccagccgg ttgaagagag ccacggggtg
    10021 atatcgtgaa gtccctgatc tacctggaca acaacgccac cactccggtt gacccgcggg
    10081 tactcgaggc gatgttgccc tatttccggg aaaagttcgg caacgccgcc agtcgcacgc
    10141 accagttcgg ctgggaagcg gagaaagcgg tcgaccttgc ccgcgagcag gtggccgctc
    10201 tgatcaacgc gagtgccaag gagatcatat ggaccagcgg ggcgactgag agcaacaaca
    10261 ttgcggtcaa aggtgtggcc cagatgtaca aggacaaagg ccgccacatc atcacacagg
    10321 tgaccgagca taaggccgtc ctcgatcccg cgaagtactt ggagacacaa ggtttcgagg
    10381 tcaccttttt gccggtggac cggaccggtc gtgttgacgt gaaccagttt cgcgaggcga
    10441 tacgcgcgga cacgattctg gtgtcgatca tgatggcgaa caacgagatc ggtacgatcc
    10501 agccgatcgc cgaaatcggg gcggtgtgca aggaaaaggg ggtcattttt cacacggacg
    10561 ccacgcaggc ctttggcaag gttccgatcg acgtgaaagc catgggcatt gatttgctga
    10621 gctgttcggg ccacaaggtt tacggcccga agggggtggg ggccctatac gtccgccgta
    10681 aaggccctcg cgtccgttgc gagcccattc tgcatggcgg agggcacgag cgaggcatgc
    10741 gttccgggac gctgaatgtt ccggggatcg tcggtttcgg caaggccgcg gagatcgcgg
    10801 ggcaggagat gcaggcggag tcggcccggc aggcaaggct gcgggatcgg ttgtgggccg
    10861 gtttgagcga gcaccttgac gagatttacc gcaacgggca cacggtgcat tgcctgccga
    10921 atacgctgaa tgtcagtttc gcgtacgtcg aaggcgaggc gctgatgatg gggttcagta
    10981 acatcgccgt gagcagcggc tcggcctgta ccagtgcgtc gctggagccg agctacgtgc
    11041 tcaaggccct tgaggtggca gacgagctgg cgcacagttc cattcgtttc agtctcggtc
    11101 ggttcaacac ggcggctgag atcgagcaga ccattgacca ggtggcggcc acagtgcgcc
    11161 gcctgcggga aatgagcccc ttgtacgaga tggccaagga aggggtcgac attaaggggg
    11221 ttgactggag ccggatttga acgaagcctg gggtgccggt ctggtcggcg cggtccctgt
    11281 ggagtattgg gatggcatac agcgataaag tgatcgatca ctacaacaat ccgcggaaca
    11341 tcggttccct ggataagaac aacccgcgtg tcgggacggg tatcgtggga gctcccgagt
    11401 gcggcgacgt gatgaaactg cagattcagg ttgatgacga cggctgtatc gtcgatgcca
    11461 agttcaaaac cttcggctgc ggcagcgcga tcgccagcag ttcgctggcg accgagtgga
    11521 tcaagggcaa aacgctcgat gaggcgctgg agatcaagaa taccgatatt gttcgcgaac
    11581 tggccttgcc cccggtgaag attcattgca gcgtgctggc ggaggacgcc atccgggccg
    11641 cgattcagga tctcaagcgg aagcaggcca atgctccggc gacagccgga gcccaggtgg
    11701 cgcgtggttg aggtatggac agggcataga atttcctggg gcttgcggac gcgtgcgtgc
    11761 gaaggcagat tgaatcgccc cggttttttg aaatggcatt cgacagcgga ggaacaaaac
    11821 agatggcgtt ggcactcacg gaacgagcgg cacaggaagt caaagcgatc ctgcaacagc
    11881 agaacctatc gccggacagc gtttatcttc gtttaggcgc caagggcggc ggatgcagcg
    11941 ggttcaccta tgcgttggac ctggttgata ccaagggtga gctcgaccag gaatacgtat
    12001 cgcatgatat tcgggtgatt tgtgacccca agagcctgat ctacctcgac ggcaccaccc
    12061 tggacttcaa ggacgagctc atggggcggg ggttcgtatt tgacaacccg aatgcatcgg
    12121 gcacatgcag ttgcggcaac agtttctgca cctgacggta cggaccaatc gcccgtcggc
    12181 ggcctgacca ggcaccgatt cggattgacc cttcgttgac gggggtttcc ggcgtgagct
    12241 cacaaccaac cacgagccgt ccgattcgct gtaccacgtg ctcccagccg ttggaatcgc
    12301 cgatctgttg taccagttgc gggcagctca acgcggatcc gatcgggcgg tgcagctact
    12361 tcgagctgtt tggcctgccc gttgattttg acattgacga gcagagcctg caccggacgt
    12421 acctctcttt gacccggtcc gtccatcccg acgtcgccgg gcacgcgtcg caggagaagc
    12481 ggcacgacgc cttagcgatc agctctgaga tgaaccgggc atacctgacg cttcgtgacc
    12541 cggttctgcg ggcggagtac ctgctgagcc ttgtcggcga gccgggcact tctgaggatc
    12601 gagcggtgtc gccggacctg ctcggagagg tcatgacgat tcgcgaggag atcgaagaag
    12661 cgcgccaggc cggcgaccag gcgagcctgg aagatctgaa gcagcaagtg gcggcaaagc
    12721 agcaagcctg catggaatcg atcgtctcgc ttgcccgcgc gcttgtcgag aagcccgatg
    12781 cccgccgtgc gctccgccgg cagctcaatg cgatcaagta ctggaacaac ctgatggacc
    12841 gcctgttgcc ggggagcgat ggggtctgac ggagaggtta tgagcgacaa tcgcgacgtc
    12901 atcgtcggta tcgatctggg gacgaccaat tcactggtgg cggtcttcga tgaggccggg
    12961 ccgcgggtga ttcgcgacga aatgggggaa agccgggttc cgtcggtgct ggcattctcg
    13021 gcggaaggcc gggtcacgat cggctgggag gcaaaagccc acgccgtcga aaaccccacc
    13081 agcacggttt tttcgatcaa gcggttgatg ggtcgaggct acgaggagct ggcggccagc
    13141 ggcgaactcg gccatctgcc ataccaggtc gtcaagcggg tgaccgacag cagcagccgc
    13201 gacatagcgg cggtcaatgt gaatggtcgc ctgatgacgc ctcccgagat cagtgctctc
    13261 attcttcgcg agctgaaacg ccgcgccgag gcccacctgg gtcggccggt cagcagggcg
    13321 gtcattacgg tgccggctta ctttgatgat gctcaacggc aggccacgcg tgatgccggc
    13381 cggattgccg gactggaggt ggtccggatc gtcaatgaac cgactgccgc ggctctggcc
    13441 tatggtcttg gtctgcgggg gcagatggac cgaatcaagg ccgccggctc gatgttgacg
    13501 ctggccggtc agtgtccgac cagccgcgac ggcgcggatg cggaggcaca gggcaggtgt
    13561 agcaagccgg ggggcggcga atcgctcatt gcggtgtacg accttggcgg cgggaccttt
    13621 gacatctcga tcctgcggct catggacggc gtcttcgagg tgcagagcac tcacggtaac
    13681 acgcgtcttg gcggcgacga cttcgatcgg gagatcatcg ggctcgttca gcgggaggtg
    13741 ggggagcagt tcggactgga gatcaactcg cctgcgatcc gccaggccct gcgaaacttc
    13801 gctgagagcg tcaagatgag gctgagctgc gacgagacgg ccaccatcga gatcgacttg
    13861 ggccaaggcc gccggtatcg ccggagtatc agccgcagcg agtttgaggc gatgatcgag
    13921 ccgtggatcg ccaaaacgct tgaatcctgc cggcgcgcgc tggcggacgc gaaggtcaag
    13981 gccgccgagg tggaccaagt gatcctggtc ggcggaagca cgcggattcc gctggttcgc
    14041 cggcgcgtgg aggaattgtt cggccgtcgt ccgtacaccg ctctgaatcc tgacgaagtt
    14101 gtcgcccttg gtgccgccgt gcaggcggcg attctggccg gcgagcagcg agatgcccta
    14161 ctgctcgacg taattccgtt gtcgctgggc attgagacga tgggcggagc gatgggcaag
    14221 ctgattctcc gcaacacccg catcccctgc caggccacgg agcggttcag caccttcgtc
    14281 gacggccaga ccaacgttaa gatcaatgtc ctgcagggcg agcgggaaat ggcgaaggac
    14341 tgccgctcgc tcggcgaatt cgaacttcgc gggataccgc cgatgccggc cggcctgccc
    14401 aagatcctgg tcacgtttct gatcgacgaa aacgggattc tgaacgtttc ggcccgcgag
    14461 gaacgaagcg gcaccgaagc gagcatccag attgtgccga cctacgggct gacctccgac
    14521 gaggtcaagc ggatggagat ggagtcctac gagcacgctc gcgaggatat gaccgctcat
    14581 cggcttatcg acttgcgaaa ccagatcgag ttcgacactc acaagaccga gcaaatgctc
    14641 gcgaaggtcg gcgaccggct tttgcccgac gagcggcaga agatcgaaac cgcgatggcc
    14701 gagttgaagg ctcttgccgc ggcgaacggc accgatgcgg acgctttaca caaagccttg
    14761 atcgagtttg accgtaagac ggttcggctc
//
LOCUS       DAOTPW010000140        14613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4028_length_14613_cov_5.165064, whole genome shotgun sequence.
ACCESSION   DAOTPW010000140 DAOTPW010000000
VERSION     DAOTPW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14613
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_14613_cov_5.165064"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..617
                     /locus_tag="P5316_13270"
     CDS             213..617
                     /locus_tag="P5316_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR85974.1"
                     /translation="MRALKRTEWVGAVLLAVAAPGCGAGGWESNLEAGLSRAASERRP
                     ALVMFSSAVSPDCVQMDEEVFSDPQVQNVLNSYVRVRIDYLIHHKLAKELNVRSVPTF
                     VIFRPDRSVAGVREGKMDARSFSFFLIKHRYY"
     gene            complement(663..2642)
                     /locus_tag="P5316_13275"
     CDS             complement(663..2642)
                     /locus_tag="P5316_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRR85975.1"
                     /translation="MPSEPTHEVLPSRTAQQTVPHPSPRDAPRNEHRLGRASLAALSL
                     GALGVVYGDIGTSPLYALRECFGPLFGVEADPRNVLGLLSLFFWSLTLVVVGKYIGFV
                     LRADNRGEGGILSLMALAVPQTVRPANLLSKAGIAIGLGLFGASLLFAEGLITPAISV
                     LSAVEGLEVSAPTLKPFIVPITLAILVALFMVQKHGTGFVGAMFGPVMLLWFGAIAAL
                     GLRWIVREPAVLTAVYPWYAVRHLIEHGWHGFLVLGAVVLCFTGTEALYADLGHFGRN
                     PIRLTWYLVVFPALLLNYFGQGAAVIHTQGAAASNPFYALATGSLHYPLVTIATLATV
                     IASQALISGAFSLARQAVQLGYCPRLTVVHTSSRTIGQIYIPEINAILMVACCGLVLA
                     FRTSSRLAAAYGVAVVSTMTLTSLLFLLMARRHWGWSPLKAAGLTAVFLTVDLTFLVA
                     NLTKLFSGGWFPLVVAMGVFGLMTTWKRGRSLLAASLAKARLPLDIFLRNLAAGKQPV
                     RVPGTAVFMTSDPTGTPVVLMHHFKHNKVLHERVILLSVSTEEVPTVPPSSRVQVQDL
                     GLGFFQVNAFYGYMQTPSVADILRCCEPAGLKVDLAEVSFYLGRETLIRTRKPGMSQW
                     RKTLFAVMSRNAISAVAYFGIPPNRVVELGAQIEL"
     gene            3032..4139
                     /gene="prfB"
                     /locus_tag="P5316_13280"
     CDS             join(3032..3103,3105..4139)
                     /gene="prfB"
                     /locus_tag="P5316_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_145129741.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRR85976.1"
                     /translation="MRFEDPAATLKDFSTRILTIRDSLDLPAKLKRLAELDEIMARRG
                     FWDVPERAREVVGELKALKGLVEPVQALLAKVEDAEALLELAKEANDAASFEELDNEL
                     HVIQQQLERVELMALLSGKNDACDCFFSIQAGAGGTEACDWAEMLLRMYLRYFENNGY
                     KAEELARTDGEEAGIRSITLKVTGYYAMGYLSCERGVHRLVRISPFDANKRRHTSFAA
                     VDVLPVLEDLDVDLKEEELDIVYFRRASGAGGQNVNKVATAVRVRHVPTGIVVECVNE
                     RSQQQNKRMALAILQSKIEALRQAERDQEMQNLYGDKGEVAWGNQIRSYVLDDRRVKD
                     HRNGVETSNVERVLDGDIQMFIEAELRRRASKRK"
     gene            4221..4892
                     /gene="elbB"
                     /locus_tag="P5316_13285"
     CDS             4221..4892
                     /gene="elbB"
                     /locus_tag="P5316_13285"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HRR85977.1"
                     /translation="MMVKVAVCLSGCGVFDGSEIHEAVLTLLALDQAGARYVCCAPNA
                     DQAVVMNHLTRNPVPGERRNVLVESARIARGEIQDLANVHARDVDALIFPGGLGAAQN
                     LSSFAADGANCTVHPEVKRLVGEMIDAGKPVGAICIAPAMLARILSSRGLVAQVTIGN
                     DARTASEIEKMGIRHTDCSCTSFVVDSAHRVVTTPAYMLAKGPAEVFEGVKGLVGEVV
                     RLAGS"
     gene            complement(5011..5637)
                     /locus_tag="P5316_13290"
     CDS             complement(5011..5637)
                     /locus_tag="P5316_13290"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRR85978.1"
                     /translation="MVDFFLTALVSILFVVDPPGAVPTYLVISAGQDAAQRKRTALRA
                     SVVATLTLAGFAGGGQVIFHYLGLTLPAFQIAGGLILFLVALDMIRAQRLTQEEPDEV
                     SEGMAKEDVAITPLAIPMLAGPASMASVTVLIDRAAGPHAILIVYVAVAITGLVSYVT
                     LRLADPLYKALGKTGIHIFSRILGLILAGVSVQFVLDGLRAGKYINAG"
     gene            complement(5718..6575)
                     /locus_tag="P5316_13295"
     CDS             complement(5718..6575)
                     /locus_tag="P5316_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR85979.1"
                     /translation="MTTVRAAIDTFRRATDLNLEDPIRSGSLLCPPDYSQVVMTGDMH
                     GHRRNFEKLIKYCQLECTPTRHVLLHELIHEQPQQEGWVDRSVELLLDAAKWKTFFPD
                     QVHFLQSNHELAQLQNHEITKGGRMVTDDFERGVAEVLDSNDIDEVLIAIDDFIASYP
                     LAARSPNGIFFSHSLPNVSHMDDFDPALVHEPADALDLSEGGMVYQMVWGRRHTPALL
                     ERLAHAFEVHYFISGHQPQEFGYEVRFDRMIILASDHNHGVFLPVDCRKQYTIEDLVQ
                     RIRPFAGVI"
     gene            complement(6572..7480)
                     /locus_tag="P5316_13300"
     CDS             complement(6572..7480)
                     /locus_tag="P5316_13300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRR85980.1"
                     /translation="MSFLVPGYIVHERLGVGARATIWLVANQQTNELFALKRVIRRAT
                     DDNRYLNQARNDFRISSMLDNPYLRRSFELRRIRRLFQLRELHIIMEYVDGRTLEEIG
                     PLGTHGLVGIFIKVAKGLHALHQMGYVHADIKPNNIILDANGNPKIIDFGQSCPIGHI
                     KNRIQGTPDYIAPEQVERGMPLDQRTDVYNLGATLYWAITGQAFPTVMPSKKRRAGID
                     LAGPRDATMPHELNPNIPVALSRLVMDCCRPNPKDRPADMREVIHRLELGQQIMDKNE
                     ATLTGPVVRRKSPLGQLSASNGKTAR"
     gene            complement(7831..8271)
                     /locus_tag="P5316_13305"
     CDS             complement(7831..8271)
                     /locus_tag="P5316_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85981.1"
                     /translation="MTLGAAFLDWFQPNQPASETPRRQLIARIRSAIHPTAEGSTPVE
                     RWHVIRAVAASDNSGVHFIVDRDGSWTATPHWQNQKAIGEAGVIRIGLRTEGRTGEIT
                     VPQWQAACQLRNNLQEMYKITLERFIPSPGLRLPSGTGDTSMVR"
     gene            complement(8658..9875)
                     /locus_tag="P5316_13310"
     CDS             complement(8658..9875)
                     /locus_tag="P5316_13310"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HRR85982.1"
                     /translation="MRGIIAAITSRIALTASTASIVCGSAFAVARADRLTESRPALST
                     TQPGVAEYQRGVRINWRDREVEVDGQVVLREGALELFACSPLTREHESIVCIDARPLH
                     VFQALGLIGLTPGRPSDFDPDTGRYSPARGDPVEIEVRYLQDGRVRREPIERWMRRAK
                     PGDKPGTGLDLPPQPWVFAGSYTTDNGSFAADPEGTVIAVVDFSSSIVALPEYHSDSN
                     AELWLVPATERIPPLYSRCTLVFRHGPVRIRLDSVGRVEISQRTMTMAEATRTIQNIR
                     GENPNVRLWLTVDPQCPADRETAFRAILDRLKIPAESITISRAPTSGPVEHNPQGMLQ
                     WVRKTTSSETKPSTAQPPWAAAARRVIGELATRTRIFEARTENWVGYLQQLRAGLRTA
                     PITSTAPSTQPNQ"
     gene            complement(9851..10792)
                     /locus_tag="P5316_13315"
     CDS             complement(9851..10792)
                     /locus_tag="P5316_13315"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRR85983.1"
                     /translation="MAAEIIDGKALAERMRAEIRHQVKTLAAEGRQPKLAAIIVGEPA
                     AGMVYARSQARQCSDMGIEYELVQVPEACGAGCLCEQIDRLNRDDSVTGIMLHLPLPP
                     EIDAPSMQYRIDPYKDVEGVNPANIGMVFYGQPIIAPCTALAVVELIKETGMQLRGAN
                     AVVVGQGAIVGLPITVFLVQQFATVTGCHVATRDLAAHTRQADLLVVAVGKAGLIGAE
                     HVKPGAVVIDVGINQTQTTDANGKTISKTVGDVDFDAVRKVASAITPVPGGVGPVTVA
                     MLLRNTVEAARKQLARRQLANPKDTTGQSSCAASSRL"
     gene            complement(10923..12383)
                     /locus_tag="P5316_13320"
     CDS             complement(10923..12383)
                     /locus_tag="P5316_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRR85984.1"
                     /translation="MTCRTLALIIVAAVAAPVFAQESAKPETGVRLTIYNDNYALVKD
                     RRMLDEPLKAGINLIRFRDVAGTIDATSVYFRSLTDAEASVVEQNYEFDLVNADKLLR
                     KYIDKPITAHTADGQVYEGTLMSFDQRQLVLAKDKEKGPICMVERGENIKRIQFSSLP
                     EGLLTRPTLVWELATGKEGKHTVEVSYIANNIRWRADYNLVLNPDDTKIDVSGWVTLE
                     NNTGTAYKDAAVKLLAGDPKIDFRNFNFGFGQDYYKIMMTKLAPTTAKGEDVSRAFGE
                     YRMYTLPEPTTVNNNQIKQIELINSPGVAVTKTFLYDGAKMQWSWGGWYHDPGFGREE
                     NKKVNVLIEIENRADRNLGIALPKGKCRVYKKDIDGALEFIGEDQIDHTSRDERVVLY
                     IGDAFDIVGERKQTDFRKISNTVFEEAFEIKVRNHKKEAVTVKILEKLYRWSEWEIIK
                     KSSDFEQLNARTVIFPVEIKPDGEAVVTYRVRYRHP"
     gene            complement(12758..14104)
                     /locus_tag="P5316_13325"
     CDS             complement(12758..14104)
                     /locus_tag="P5316_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRR85985.1"
                     /translation="MSAQTTQPANLTEGVGLTVYNQDFAIVRERRTMPLAAGRSEVKF
                     KDVAATIVADTVHFLSLRPAGDAKVIEQNYEFDLVSANKLLDKYIDKDIGLITRDGSL
                     IEGKLMSFDDGQVVLAVRDGIQMVPRGKNVRDIRFSALPGGLLTRPTLVWLVNAKQAS
                     DHLVEVSYRANAVNWRVDYRALADADGKMLDLSGWVTIDNRSGRTYEDARLKLMAGEV
                     HVVKPQEERFRRRIAAGVEVAFARLDTPAFEEKAFAEYHLYELSRPATIKDQQVKQIE
                     LINIEQIPVKRKYQYRGEGDNVHVILEFKNAKETREGLGIPLPKGPIRVYQMDADKQN
                     EFIGMDSIEHTPKNEDIKIRIGTAFDIKGERVQTNLRRPGEGVEDKDWRIRLRNHKDQ
                     PVKVEVLERLEVGGRNWEIREASHEWKKKDFQTITFDVNVPANGEVTITYSVEYTW"
BASE COUNT         2629 a         4286 c         4714 g         2984 t
ORIGIN      
        1 gtcggggatg ccgccgcggt gctcaagcga caaggcatcg agtgtttggc cgtttttcgc
       61 gatcgtaagc ggaccgacga accgctttga cgttcgcggc gcggggggtg aaggtcacgg
      121 tcgtcgtcct cgacttgcat tggcgggggg atgtggcaga atgccatggt caggaggaat
      181 cggttcgcca ggggccgtgc cgggaacaga ctatgagagc actgaagcgg acagagtggg
      241 tcggtgcggt cctcttggcg gttgcggctc ccgggtgcgg cgctgggggg tgggaaagca
      301 acctcgaggc gggcttgtcg agagcggcca gcgagcggcg acccgccctg gtgatgttca
      361 gttcggcggt gagccccgat tgcgtccaga tggacgaaga ggtgttttcc gatccgcagg
      421 ttcagaacgt cctgaactcc tacgtgcggg ttcggatcga ctacctgatt caccacaagc
      481 tggccaagga actgaacgtg cggagcgtgc cgacgtttgt gattttccgc ccggatcgtt
      541 cggtggccgg cgttcgggag ggtaagatgg acgcccggag cttctccttc ttcctcatca
      601 agcaccggta ctactgaccg gtcgtgcgcg tgacgactgg ggcggcgtct tcctgccgga
      661 cgtcacagct cgatctgggc gcccagttcc acgacacgat tgggcggaat gccgaaatag
      721 gcaaccgccg aaatggcgtt tcgcgacatc accgcgaaga gagtcttgcg ccattgcgac
      781 atgcccggtt ttcgggttct gatcaacgtt tcccttccaa gatagaagct cacctccgcc
      841 aggtcgactt ttagtccggc cggttcacag cagcggagta tgtcggccac actgggcgtc
      901 tgcatgtagc cgtagaaggc gttgacctga aagaatccca ggcccagatc ctgcacctga
      961 acccgagagg agggaggcac cgtcggcacc tcttccgtcg agacgctcag caggatgacg
     1021 cgttcgtgga gcaccttgtt gtgtttgaaa tggtgcatga ggacgacggg cgtgccggtc
     1081 ggatccgagg tcatgaacac cgcggttccc ggcacacgga cgggttgttt tccggcggca
     1141 agattccgca agaagatatc aagcggcagc cgggccttgg cgagcgacgc ggccagcaat
     1201 gatcggccgc gcttccaggt ggtcatcagt ccaaacactc ccatggcaac caccagtggg
     1261 aaccatccgc cgctgaagag ctttgtcaga ttggcaacga ggaacgtcag gtcgactgtc
     1321 agaaacaccg cggtcagtcc ggccgccttc aggggactcc atccccagtg tcggcgagcc
     1381 atcaggagga acagcaagga ggtcagggtc atggtgctga ccacggcgac gccgtaggcc
     1441 gccgccagcc gcgaggaggt cctgaaagcc agaaccagcc cacagcatgc caccatcagg
     1501 atggcattga tctcgggaat atagatttgg ccgatggtcc ggctggacgt gtgtaccacg
     1561 gtcaggcggg gacagtaacc caattgcacc gcctgtcggg cgagggagaa cgctccggat
     1621 atcaaggcct gtgaggcgat gactgttgcc agtgtggcaa tcgtcaccag ggggtaatgg
     1681 agcgatccgg tagccagtgc atagaagggg ttgctcgccg cagcgccttg ggtgtggatc
     1741 accgccgcgc cctgaccgaa gtagttcagc agcagcgcgg gaaacaccac gaggtaccaa
     1801 gtcagccgaa taggattcct tccgaaatga ccgagatccg cgtataacgc ttccgtgcct
     1861 gtgaagcata gtaccacggc tcccagcacg agaaagccat gccacccatg ctcgatgaga
     1921 tggcggacgg cgtaccaagg gtagacggcc gtcaataccg cgggttcccg cacgatccac
     1981 cgcagcccca acgctgcgat ggctccaaac cacaacagca tgaccggtcc gaacatcgcc
     2041 cccacaaacc ccgtcccgtg tttctgaacc atgaataacg ccaccaggat ggcaagcgtg
     2101 atcgggacaa tgaacggctt tagcgtcggc gcggagactt caaggccctc gaccgcactg
     2161 aggaccgaga tggccggcgt gatcagaccc tcggcgaaca gcagagatgc cccgaacaac
     2221 cccaggccga tcgcgatccc ggccttggaa agcagattcg ccggtcgaac cgtctgcggc
     2281 accgccaggg ccatcagtga caggatgccg ccttcaccgc ggttatcggc gcggagcacg
     2341 aagccgatat acttgccgac cactaccagg gtcagggacc agaagaaaag ggacagcagc
     2401 cccagcacgt ttcgcggatc ggcctcgacc ccgaacagtg ggccgaaaca ttctctcagg
     2461 gcgtacagag gggaggtgcc gatgtcgccg tagaccaccc cgagcgcccc tagagacagt
     2521 gcggccaggg atgcccgccc gagccgatgc tcgttgcggg gtgcatcacg aggcgaagga
     2581 tgcgggacag tctgctgcgc cgtcctggag ggcaagactt cgtgagttgg ttctgagggc
     2641 atgaacctcg atcgtctctg agaggttgtc agccaaggcc ttcaagatga ttctgcccgg
     2701 ctacttcgcc gggccgcggg ttccgattgt acagggtttg tgttaccgtg ccactccgcc
     2761 ggcggtcacc cggcaaggcg gatcaggatg gtctcttgac gggcccatcc gagtcagccg
     2821 agagcggacc agaccgcaga agagcgatac ggcagagggg cggcattcct gcggtggcgg
     2881 ctctgaaaga agccctggaa aggtccgtgg cgttttccag ccgaatgggt taaggaggcg
     2941 gtgcggccga atccgtttcg gttgttccgg ggagcgtccg cgagtatagt cagtaaccag
     3001 tggctcggat gccgctgaga ggacatgcat catgagattt gaagaccctg ctgcaacact
     3061 caaggatttc tcgacccgta tcctcaccat acgggattct ctttgacttg cctgccaagc
     3121 tcaagcgact tgcggaactg gatgagatca tggccaggcg cggcttttgg gatgtgccgg
     3181 agcgggcgcg cgaggtggtc ggcgaactga aggctctcaa ggggctggtc gagccggtgc
     3241 aagccttgct ggcgaaggtc gaggatgccg aagcgctgct cgaactggcc aaagaggcca
     3301 acgacgccgc ctcttttgag gagctggaca atgagctgca tgtgatccag cagcagctcg
     3361 agcgtgtcga gctgatggct ctgctgtccg gcaagaacga cgcgtgtgac tgtttcttca
     3421 gcatccaggc cggcgcgggc ggaaccgagg cctgcgactg ggccgagatg ctgctgcgaa
     3481 tgtaccttcg ttacttcgag aacaacgggt acaaggccga ggagctggcc cgcaccgatg
     3541 gtgaggaggc cgggatccgc agcatcaccc tcaaggtcac cggctactat gccatgggat
     3601 acctatcgtg cgagcggggc gtccatcgcc ttgtacggat cagtccgttt gatgccaaca
     3661 agcgtcggca cacgagtttc gcggcggtcg acgtcctgcc ggttctggag gaccttgacg
     3721 tcgatttgaa ggaggaggag ctggacatcg tgtactttcg gcgggccagc ggggcgggcg
     3781 gtcaaaacgt gaacaaggtg gcgacggccg ttcgcgtgcg acatgttccg acgggcatcg
     3841 tggtggagtg cgtcaacgag cgcagccagc aacagaacaa gcggatggct cttgccatcc
     3901 tccagtccaa gatcgaagct ctcaggcagg ctgagcgcga ccaagagatg cagaacctct
     3961 acggcgacaa gggggaagtt gcgtggggca accagatccg cagttacgtg ctcgatgacc
     4021 gccgggtcaa ggatcatcgc aacggcgtcg agacgagcaa cgtcgaacgc gtcttagacg
     4081 gcgatattca gatgttcatc gaagccgagc ttcggcgtcg tgccagcaag agaaagtaat
     4141 cagcagtccg tcattcgtga tcggtggccg atgggcgtct cggtcccgtc gggcggcggg
     4201 tgccggcgta aaggagcgag atcatggtga aggtcgcggt ctgtttatcg ggatgcggcg
     4261 tgttcgacgg ttcggagatt cacgaggcgg tcttgaccct gcttgccctg gatcaggcgg
     4321 gcgccaggta cgtgtgttgt gctcccaacg cggaccaggc ggtggtgatg aaccacttga
     4381 cccgcaaccc tgttccgggg gagcgtcgca acgtgctggt cgaatcggcg cggattgccc
     4441 ggggcgagat ccaggatctg gccaacgttc acgcccgcga tgttgatgca ctgatctttc
     4501 cgggcggcct cggggcggcc caaaacctgt cttcattcgc cgccgacggg gcgaactgta
     4561 cggtgcatcc cgaggtgaag cggctggtcg gagagatgat tgacgcgggc aaacccgttg
     4621 gggccatctg catcgccccg gccatgctcg ctcggattct gagcagcagg gggctggtgg
     4681 cgcaggtgac catcggcaac gacgcccgga cggcctctga aatcgagaag atgggaattc
     4741 gtcacaccga ttgttcctgc acgtcgtttg tcgttgactc agcacaccgt gtggtgacga
     4801 cacctgccta catgcttgcc aaagggcctg cggaggtgtt tgagggagtc aaagggttgg
     4861 ttggggaggt tgttcgtctg gcaggctctt agggtcgaat tgcagggggt tactcatgct
     4921 cgcgtcgcct gagctcgggt tttgcccgcc tcggcagcgg cgagacggat acaagcttgc
     4981 cggtgtggag gtcgtccggc gaggggaagg ctaccccgca ttgatgtact tgcctgcccg
     5041 gagcccgtcg agaacgaact gaacggagac gcctgcgaga atgaggccca gaatacggct
     5101 gaaaatgtgg atgccggtct tgcccagggc tttgtagagg gggtcggcca gtcgcagggt
     5161 gacatagctc accaggccgg tgatcgcgac ggcaacataa acgatgagta tggcgtgagg
     5221 gccggcggcg cgatcgatca gcacggtgac gctggccatt gaggctggtc cggccagcat
     5281 gggtatcgcc aggggggtga tggccacatc ctctttggcc atgccttcgc tgacttcgtc
     5341 gggctcttcc tgggtgagcc gctgagcccg gatcatgtcc agtgcaacga gaaacaagat
     5401 cagaccgcct gcgatctgga aggcgggcaa cgtcaggccg aggtagtgaa agatcacctg
     5461 tccgccgccg gcgaatcccg ccagcgtgag cgttgcgacg acactggccc gcagggcggt
     5521 tcgttttcgc tgtgcggcgt cctggccggc actgatgacc aggtatgttg gcacggcgcc
     5581 gggggggtcc accacaaaga ggatggagac cagtgctgtc aggaagaaat cgaccatggc
     5641 acggcgctca tttgaccgcg ccggcgggcc ggccgcaagt gcgcgggcag ggcgccgccg
     5701 ggaggtggag agccggatca gatgacgcct gcgaaggggc gaatccgctg gaccaggtcc
     5761 tcgatggtgt attgcttgcg gcagtcgacc ggcaggaaca cgccgtgatt gtgatcgctg
     5821 gcaagaatga tcatccggtc gaaacggacc tcatagccga attcctgggg ctgatgcccg
     5881 gagatgaagt aatgaacttc aaaagcgtgc gccagacgtt ccaggagggc cggggtgtgg
     5941 cggcgtcccc agaccatctg gtagaccatt cctccttcgg acagatcgag cgcgtcagcc
     6001 ggctcgtgca cgagcgccgg atcgaagtca tccatgtgcg agacgttagg cagcgaatga
     6061 gagaagaata tgccgttggg cgaccgggcc gccaggggat acgaggcgat gaagtcgtcg
     6121 atcgcgatga gcacttcgtc gatgtcgttg gagtcgagaa cctcggccac cccgcgttcg
     6181 aagtcgtcgg tcaccatgcg tccgcccttg gtgatctcgt ggttctgcag ttgggccagt
     6241 tcgtggttgc tttgcaggaa atgaacctgg tcggggaaga acgttttcca cttggccgcg
     6301 tcgagcagaa gctcgacgga gcgatcgacc cacccttcct gttgaggctg ttcgtggatc
     6361 agttcgtgta gcagcacgtg gcgcgtaggg gtacattcga gctgacagta cttgatgagt
     6421 ttctcgaagt tgcggcgatg cccgtgcatg tccccggtca tgaccacctg actgtagtcg
     6481 ggcgggcaaa gcagggaacc ggagcggatc gggtcctcaa gattgaggtc cgttgcgcgg
     6541 cgaaacgtgt caatggcggc acgaacggtt gtcatctggc cgtcttaccg tttgaagcgg
     6601 acaactggcc gagcggactc tttcgtcgca cgaccggacc cgtcaaggtt gcctcgttct
     6661 tgtccatgat ctgttgaccg agttcgaggc ggtgtatgac ttctcgcatg tcggccggac
     6721 ggtccttggg gttcgggcgg caacagtcca tgacgagacg actcagggca acggggatgt
     6781 tgggattcag ctcgtgaggc atcgttgcgt cccgtggtcc cgcgagatca ataccggctc
     6841 tgcgtttttt gctcggcatg acggtcggaa aggcctgacc ggtgattgcc cagtagagcg
     6901 tggcgcccag attgtaaacg tccgtccgct gatccagcgg catgcctcgc tcgacctgct
     6961 cgggtgcaat gtagtcgggt gttccctgga tgcggttttt gatatggccg atcgggcaac
     7021 tttggccgaa atcgatgatc ttcggattgc cgttcgcgtc gagaatgatg ttgtttggct
     7081 tgatgtcggc gtggacgtag cccatctggt gaagggcgtg cagacctttg gccactttga
     7141 tgaagattcc gaccagcccg tgcgttccga ggggcccgat ttcctccaag gtccggccgt
     7201 cgacgtattc catgatgatg tgcagctcgc gcaactggaa caggcgcctg attcgccgca
     7261 actcgaagct ccgccggaga taggggttgt cgagcatgga ggagatccgg aaatcgttcc
     7321 tcgcctgatt gagatagcgg ttgtcatcgg tggcccggcg aatcacccgc ttgagggcga
     7381 aaagctcgtt ggtctgctgg ttggcgacca accaaatggt tgcccgcgca cccacgccca
     7441 agcgctcatg cacgatgtaa ccgggtacca ggaaactcac ctaactatcc cctaacatga
     7501 gccgtcggtc tacaactggt tgcggcccga agcagccaat cgacaggagc ttgtgcccca
     7561 agtgcatttt aagtagcccg tttgctgacc gtcaacgggt ggaagacacc gaagcgtctg
     7621 agtaaccaga cgatgtgctt gccgcaagct gcggctgcaa tgcgtcggcc gtatcgatcc
     7681 ggcaggcggt cggcttgttc cgccccggtt ggctgtatcg gctcgattct tgccggcggt
     7741 tagcacgcgc cggtttatcg gcactatgca aaaacgcctc ccggactcat gcgtcctgaa
     7801 ggcgtttcga tcgcagaaga cttgcgaatg ctatctgacc atgctcgtgt cgccggtccc
     7861 ggagggaagc cgaagaccgg ggctggggat gaagcgttcg agggtgatct tgtacatttc
     7921 ctgaaggttg ttgcgtaact ggcaggcagc ctgccactgc ggcacggtga tttcgccggt
     7981 tcgcccctcg gttcgcaacc cgatgcggat cacgcccgct tctccaatgg ctttctgatt
     8041 ctgccaatga ggcgtggcgg tccatgatcc gtcccgatcg acgatgaaat ggacgccgga
     8101 gttgtcggag gcggccacgg cgcggatcac atgccacctc tcgacggggg tgcttccctc
     8161 ggctgtcggg tgtatggcgc tgcggatcct ggcgatcagt tgtcggcggg gtgtttccga
     8221 agccggttgg ttcggctgaa accaatccag gaaggctgcc cctaacgtca tggaagcgac
     8281 cagacaccct aacatctttg ccagacgttt atgctggtcc cgaggtgtac gtgcgacgga
     8341 catgttgagc tgaagccccg ttgccaatac tgctaagcta ctgaccgcaa gaatctataa
     8401 tatacgtatc ggacaaacgc aacatctatt tggtgtcttt ttgcgttttc cggcgaactt
     8461 ttctcaagtt cctcgttttt ttccgcgcgc cccggcgccg agccgtggtc cggcgagcgc
     8521 aattatcggt ctggctcgtc gcgccgtcgt gcgtcttgac gacttgctcg ggcgggctga
     8581 tttgccgctt tccccctcca gaactcggtc aacgcctgtg agtttctgag cgaagctttg
     8641 cacttttcca gcagaaatca ttgatttggc tgcgtgcttg gggcggttga cgttatgggg
     8701 gctgttcgga ggccggccct caactgttga agatatccga cccagttctc ggtcctggct
     8761 tcgaagattc gcgtacgggt ggccagttcc ccgatgactc tcctcgcggc cgcggcccag
     8821 ggcggctgtg cggtagacgg cttggtttcc gaggaggtcg ttttgcggac ccactggagc
     8881 atcccttgcg ggttatgctc gaccgggccc gaggtgggcg ccctggagat ggtgatcgat
     8941 tcggcgggta tcttgagccg atccaggatc gcgcgaaaag cggtttcccg gtcagcgggg
     9001 cattgcggat cgacagtgag ccatagtcgg acgttcgggt tttcaccccg gatgttttgg
     9061 atggtccggg ttgcctcggc catagtcatg gtccgttgcg atatctccac tcgtccgacc
     9121 gaatcgagcc ttatgcggac cggtccatgc cgaaagacga gcgtgcagcg ggagtagagg
     9181 ggagggatac gctctgtggc cggaaccagc cacaattccg cgttgctgtc ggagtgatac
     9241 tctgggagag cgacgatcga actgctgaaa tcgaccacgg cgatgacggt gccttccggg
     9301 tcggccgcaa atgacccgtt gtcggtggtg tatgagcccg caaagaccca gggttgaggc
     9361 ggcagatcga gaccggttcc cggcttgtcg ccgggcttgg cgcggcgcat ccatctttcg
     9421 atcggttcgc ggcggacgcg cccgtcctga aggtaacgaa cctcgatttc caccggatca
     9481 cccctggcgg gggagtaccg gccggtatcc gggtcgaaat cgcttggcct gccgggtgta
     9541 aggccgatca gaccaagggc ctggaagacg tgcagagggc gagcatcaat gcacacgatg
     9601 ctctcgtgct cgcgcgtcag gggcgagcag gcgaacagct cgagtgcccc ttcgcgcaag
     9661 acgacctgcc cgtcgacttc tacctctcga tctcgccagt tgattcgcac gccgcgctga
     9721 tactctgcga cccctggttg cgtcgttgat agtgccgggc gggattccgt cagtcggtcg
     9781 gcgcgggcga cggcgaaagc agacccacag acgatgctgg ccgtggaggc cgtcagcgca
     9841 attcgggaag ttatagccgc gatgatgccg cgcatgaaga ttgtccggtg gtatccttgg
     9901 gattggccag ttgacgtcgg gccaactgtt ttcgtgcggc ttcgaccgtg ttccgcagca
     9961 acatggcgac ggtcaccggg ccgacgccgc cgggcacggg ggtgatggcc gaggccacct
    10021 tgcgcacggc gtcgaagtcg acatcgccga cggtcttgga gatcgtcttg ccattcgcgt
    10081 cggtggtttg tgtctggttg atccccacat caatgaccac tgctcccggt ttgacatgct
    10141 cggcaccgat caagccggcc ttgccgactg cgacaacgag caggtctgcc tgtcgggtgt
    10201 gggccgccag gtcgcgggtg gccacgtggc agccggtgac ggtggcgaac tgctggacaa
    10261 ggaacacggt gatcggcaat ccgacgatgg caccttggcc gacgaccacc gcgttggctc
    10321 cgcggagctg catgccggtc tccttgatca gctcgacgac cgccagggcg gtacaggggg
    10381 cgatgatcgg ctggccgtag aaaaccatgc cgatgttcgc cgggttcacg ccctctacgt
    10441 ccttgtaagg gtcgatacgg tactgcatgg agggggcgtc gatttccggt ggcagtggca
    10501 ggtgcagcat gatgcccgtg acgctgtcgt cacgattcag ccggtcgatt tgctcgcaga
    10561 ggcatccggc accgcaagcc tcaggcacct ggaccagctc gtattcgatg cccatatccg
    10621 agcattggcg ggcttgtgaa cgggcataca ccatgcctgc cgccggctcg ccgacgatga
    10681 tcgccgccag cttgggctgg cgtccttcgg ctgccaaggt cttgacctgg tgccgaattt
    10741 ccgcccgcat tcgttcggca agtgctttgc cgtcaatgat ctctgctgcc atgggtcctc
    10801 cacccggcag gatgaccggt gcttggcaag gaggggatta taaatgctga ggcggattgc
    10861 gccgagggca gcggcgggca tggttgcttc ggatcgggac cggtgcaagg ccaaattgcc
    10921 ccttacggat gccgatacct tacccggtag gtgaccaccg cttccccgtc aggtttgatc
    10981 tcgacgggga agatcaccgt tcgcgcgttg agctgctcga agtccgagct cttcttgatg
    11041 atctcccact cgctccagcg gtagagcttc tcgaggatct tgaccgtcac cgcttccttc
    11101 ttgtggttgc ggaccttgat ctcgaaggcc tcctcgaaaa cggtattgga aatcttgcgg
    11161 aagtcggtct gcttgcgctc gccgacaatg tcgaaggcgt cgccgatgta gagcaccacc
    11221 cgctcgtcgc gcgaggtgtg gtcgatctga tcttcgccga tgaattccag cgccccgtcg
    11281 atgtctttct tgtacacccg gcacttgccc ttgggcaggg cgatgcccag gttgcggtcg
    11341 gcccggtttt cgatctcgat caggacgttg accttcttgt tctcctcccg cccgaagccg
    11401 gggtcgtgat accagcctcc ccacgaccac tgcatcttgg caccgtcgta caggaaggtc
    11461 ttggtcacgg ccacaccggg gctgttgatc agctcgatct gcttgatttg gttgttgttg
    11521 accgtggtgg gctctggcag cgtgtacatc cggtactcgc cgaaggcccg gctgacgtct
    11581 tcgcccttgg cggtcgtggg ggcgagcttg gtcatcatga tcttgtagta gtcctggccg
    11641 aagccgaagt tgaagttgcg gaaatcgatc ttcgggtcgc cggccagaag cttgaccgcc
    11701 gcatccttgt aggccgtgcc agtgttgttt tcgagggtga cccatccaga gacgtcgatc
    11761 ttggtgtcat cggggttcag aacgaggttg tagtccgctc gccaccggat gttgttggca
    11821 atgtagctca cctcgacggt gtgcttgccc tccttgccgg tggccagctc ccagaccagc
    11881 gtcgggcggg tcagcaggcc ctcgggcagc gagctgaact ggatgcgctt gatgttctcg
    11941 ccgcgctcga ccatgcagat cgggcctttc tccttgtctt tggcgagcac cagttgtcgc
    12001 tggtcgaagc tcatcagcgt gccctcgtac acctgcccgt cggccgtgtg ggccgtgatc
    12061 ggtttgtcaa tgtacttgcg gagcagcttg tcggcgttga ccaggtcgaa ctcgtagttc
    12121 tgctcgacca ccgaggcctc cgcgtcggtc agcgagcgga agtacacact ggtggcgtcg
    12181 atggtgccgg ctacgtctcg gaagcggatc aggttgatcc ctgccttgag cggctcatcg
    12241 agcatgcgcc ggtctttcac caaggcgtag ttgtcattgt agatggtcaa cctcacgccc
    12301 gtttccggtt tggcggattc ctgggcgaaa accggagcgg cgacggctgc gacgatgatc
    12361 agtgcgagtg tgcgacatgt catgatctgt tctcccgaag gaagctctca gcttttggct
    12421 tctggctgtt agctcctggc tctcctcgtt attcctgaac cctgaaccct caatcggcag
    12481 gtctccgtcg ccgttggcga ctccgaccat gccctacctt cctgagccct caatcctcgc
    12541 tcggccgaga tccctatctc ggccgctctc acccgcggga ttcctatccc gcgacgatgg
    12601 ggggcgaaag gattcgccgc ccgaatgggt gcgagaaagg attctcgcac cagcgacact
    12661 gaaccccgct tgccacgacg tagcctttgg cgaagccggg aaccctcaat cggcaggtct
    12721 gcgtcgccgt tggcgactcc gaccatgccc tgcctgctca ccatgtgtac tccacgctat
    12781 aggtgatcgt gacctcgcca ttggcgggca cgttcacgtc gaacgtgatc gtctggaaat
    12841 ccttcttctt ccattcgtgg ctggcctcgc ggatctccca gttgcggcca ccgacctcga
    12901 gccgttcgag tacctcgacc ttgaccggct ggtctttgtg gtttctcaga cgaatccgcc
    12961 agtccttgtc ctcgacgccc tcgccagggc gcctcaggtt cgtctgcacc cgttcgccct
    13021 tgatgtcgaa ggcggtgccg atcctgatct tgatgtcctc gttcttgggc gtgtgctcga
    13081 tggagtccat gccgatgaac tcgttctgct tgtcggcatc catctggtac acgcggatcg
    13141 gtcccttggg cagcgggatg cccaaccctt cgcgcgtttc cttggcgttt ttgaactcca
    13201 ggatcacatg cacgttgtcc ccttcgccgc gatactggta ctttcgcttg acgggaatct
    13261 gctcgatatt gatcaactcg atctgcttga cctgctggtc cttgatcgtc gccgggcgcg
    13321 acaactcgta caggtggtac tcggcgaagg ctttctcctc aaatgccggc gtgtccaagc
    13381 gagcgaacgc tacctcaacc ccggcggcga ttctcctcct aaagcgttct tcctgcggtt
    13441 tgaccacgtg cacctcgccg gccatcagct tgaggcgggc atcctcgtag gtccgcccgg
    13501 agcggttgtc gatggtcacc catcccgaca ggtcgagcat cttcccgtcg gcatcggcca
    13561 gcgcccggta gtccaccctc cagttgaccg cgttggcccg gtacgagacc tcaacgaggt
    13621 ggtcgctggc ctgcttggcg ttgaccagcc agaccagcgt cggccgggtc aacaggccgc
    13681 cgggcagggc cgagaagcgg atatcacgca cgttcttgcc ccgcgggacc atctgaatcc
    13741 cgtccctgac ggccagcacg acctggccgt cgtcgaagct catcagtttg ccctcgatga
    13801 ggcttccatc gcgcgtgatc aggccgatgt ccttgtcgat gtacttgtcc agcagcttat
    13861 tggcgctgac caggtcgaat tcatagttct gctcgatgac cttggcatca ccggccggcc
    13921 tgaggctcag gaagtggacg gtatcggcca cgatggtcgc ggccacatcc ttgaatttca
    13981 cctccgaccg cccggccgcc agcggcatcg tccgccgctc gcggacgatg gcgaaatcct
    14041 ggttgtagac cgtcagcccg acgccttcgg tcaagttcgc cggctgggtc gtctgggctg
    14101 acaccaaggg tgccaccaga agggtcgcca gcagggccag gattggcccg gtggttcccc
    14161 gtgtgccact gctctgtccc cgtgtgccac tgctctgtcc ccgtgtgcca ctgctctgtc
    14221 cccgtgtgcc actgctctgc gagcagtgca gacgggttgc tcgctggccc cagaatcctt
    14281 tctggggcct ctcgcgtgga ggattgctat cctcattctc accgaatgcg gaaaggattc
    14341 cgccaccaga ggcggcccgg aaaggattcc gggccgagca aaatccctgc tggccccaga
    14401 atcctttctg gggcctctcg cgtggaggat tgctatcctc attctcaccg aatgcggaaa
    14461 ggattccgcc accagaggcg gcccggaaag gattccgggc cgagcaaaat ccctgctggc
    14521 cccagaatcc tttctggggc ctctcgcgtg gaggattgct atcctcattc tcaccgaatg
    14581 cggaaaggat tccgccacca gaggcggccc gga
//
LOCUS       DAOTPW010000141        14547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4054_length_14547_cov_5.547750, whole genome shotgun sequence.
ACCESSION   DAOTPW010000141 DAOTPW010000000
VERSION     DAOTPW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4054_length_14547_cov_5.547750"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..1532)
                     /locus_tag="P5316_13330"
     CDS             complement(375..1532)
                     /locus_tag="P5316_13330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR85986.1"
                     /translation="MASLFSDMSHETVTAALPAYLASMGAAAGALGTIEGFADGFSSI
                     AKLYGGWLADRLRRRKVLCAGGYGFMAFSPVVIALATNWFIVLGGRVLAWVSRGVRTP
                     PRKALLADAVTPTTYGRAFGFERAMDTTGAIIAPIAVLLLLRVGLTHRGLILLSTIPA
                     VLAVFAITLLVRERKDRTPIRTPFLQSFGGFNSRFKEFLGAVGLFGLGDFADTFYILY
                     AVTVLSPDIGPGRAATLSVAGYTLHNVLYASWSYLGGWMADRTNKRLLLVAGYMAAAL
                     AAACMVAGVRSYAGLAIMFALGGTGVGLYEAVEDAVAAELLPSGIRGSGFGALAVVTG
                     IGDLVSSVVVGWLWTGFGIEVGVTYALVLMLAGAGFMLYLAGRRVDHGNHQ"
     gene            complement(1638..2243)
                     /locus_tag="P5316_13335"
     CDS             complement(1638..2243)
                     /locus_tag="P5316_13335"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRR85987.1"
                     /translation="MRPDWIQWAGNHPRWFALIVGTKRLRTKAGLLLVGFGLGEGVYN
                     HEVPFDLDRPNAWVFVGLAMVLAGVAFRFAALGSLRKKEELATTGVYSLCRHPLYLGS
                     VLMAYGFCCLLNDSKLFLIASAYFILFYGLTIAWEEIRLAQRYGDAYHRYANETPLIL
                     PVGRPCRGQFGLATALRNGGAALIAVTTILLAAIESMAEFI"
     gene            complement(2409..3923)
                     /locus_tag="P5316_13340"
     CDS             complement(2409..3923)
                     /locus_tag="P5316_13340"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HRR85988.1"
                     /translation="MIMNLLSGAFALLMTTNGLAGAPVAGEQEQRVAKPAQSPPAVGL
                     SSAEPPASRPAGRPAIRAVVDPRVELMSIIFRLAGNPEYNMPNSQSVYADEVKKHFSK
                     YRNHPVVLAAQRLRASRGVSYDAVMSMAVHVSDTVELKERVPFDQPPDAIMLDPRWKT
                     EEARDFLKQARLFVEHARFAAFVEQHRDFYASAAARLDAALGNRDLRQWFDSYFGTTA
                     EVQLRPVVGLLNGGGCYGPSFRPAAGPVEMYSIIGAYKFDHEGLPEFDSNTALTAVHE
                     FAHSYANPLVDKHLDTMLDAAQRIFSTCEDEMRRQAYGNASTLLRESMVRACTVRYVA
                     DTMGKAAAATAARQEADHGFTWVSDLSRLLAEYEGRRDRYPTLEAFMPRVVDFFNRYA
                     AGLKDQERPRVISIVPANGATDVDPDLTAIKITFSEPMVDKSWSVVGGGSNFPEITGD
                     ISYDQDRRVLTIPVKLKPSWTYRFWLNRGRFNSFRSERGAPLSSLPVEFKTRAR"
     gene            4145..6139
                     /gene="uvrB"
                     /locus_tag="P5316_13345"
     CDS             4145..6139
                     /gene="uvrB"
                     /locus_tag="P5316_13345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRR85989.1"
                     /translation="MSEFVIHSEFEPAGDQPAAIEKLTGGFQSGRKFQCLLGVTGSGK
                     TFSMAHLIRNLNRPTLVISHNKTLAAQLYDEFSSLFPENAVEYFVSYYDYYQPEAYIP
                     QRDIYIEKDASRNDDLDRLRLSATSSLVSRRDVIIVASVSCIFGLGSPEDFKRSIITI
                     RRGDRVERDELLRRFVDLQYERNDVDFRRGKFRVRGDVVELCPGNEEFAYRIELFGDE
                     VDRIDLIDPLTNETITDQDVIFIYPAVHYVMPEDRIAAAIKSIQEELDQRLMELRRMG
                     KLLEAQRLSARTRYDLEMIQEVGYCSGIENYSRHLNGTPPGAKPFTLVDYFPKDFLLI
                     LDESHVTIPQIRAMYAGDRSRKEVLVEHGFRLPSCLDNRPMRFEEFESMWNQVLFVSA
                     TPGPYELEKCGGEVVEQIIRPTGLVDPLIHVRPARGQVPDLLDRISERVAVGERVLVT
                     TLTKRLAEDLSAYVDQKGFRCKYLHSEIDTLERVEILNDLRRGDFDVLVGVNLLREGL
                     DLPEVSLVAIMDADKEGFLRSETSLIQTIGRTARNVNAEVILYADKTTPSMQRAIDET
                     NRRREIQLKYNEEHKITPETIKKAIRSSLEQEISARKIAREAVHAAEDEYDRSELIAA
                     LEQEMLEAAERLDFERAAVLRDRIRQLETGPQAQRADAGR"
     gene            6364..7260
                     /gene="nadC"
                     /locus_tag="P5316_13350"
     CDS             6364..7260
                     /gene="nadC"
                     /locus_tag="P5316_13350"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRR85990.1"
                     /translation="MAQMIDRERLRRQWEDALAEDGFDRDITSLTTVDDAATGTARLV
                     ARQGGVFAGAVIFDLLREAYSHKLTVTVNAQDGANMRKGEGIAVLAGPMRLLLGIERT
                     LLNFLQRLCGVASLTRRYVDAVGGTQARIYDTRKTIPGWRQLDKYAVRCGGGWNHRMG
                     LHDAILVKDNHLAGVSIDSLAVALFDMVREAAALAPPPAFIEVEVDTIEQLEEVFKVS
                     GVDVILLDNFSIAQMRQAVRRRDELGLAGRVQFEASGGVDLDSVRGIAETGVERIAVG
                     ALTHSSGALDIAMDLETTRLSG"
     gene            complement(7304..10501)
                     /locus_tag="P5316_13355"
     CDS             complement(7304..10501)
                     /locus_tag="P5316_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRR85991.1"
                     /translation="MLRTYGGFPYRYLACLMVLSAAGFITRYTFAETVIPSSSWRNTI
                     TFPSDSFRKAAPETGDPGWIKFLIFVSDPTKVYFQDSNAYPFHYDWATTFVQEYIGMS
                     TEEFNQISLYAEGQEAILGSVILPYWTVHQPPSEQIKEYGIELVRRDAYDPAQVVNLF
                     NLVKSKITAEAGVEAFYFPSYDQIASAEENRAYFEANGIIVSSPSRWAKGNISYSRGW
                     ALGTLKQFAATQIENAYLTGQLAPGDILLTDGVPADMPVIAGIITLAPSTPNSHVAIL
                     ANTYGIPFVFLALSEDATRAQQLVGRKIALRVYGNANDCDARLIDVQDVLTPQQIDEI
                     LALKTPPPLNICPVTPYGAYSASTDGLWPSDICYFGGKAANFGLLRRSIPSNCPVAMA
                     FSFDLWNEFLSQTLTNGRTLREEIAYRLAGFTYPPANMAALADVLEGIREDLFKKTSA
                     TVFSPALQNEIISALQDPAYGFDPYKKIRFRSSTNVEDSEQFTGAGLYDSFSGCLADD
                     LDGDGVGPSLCDPAEANERGVFRAIRRVFASFYYDNAYLERLRHGIDESQAGMAMLVH
                     HSTPDPIEMANGVATLERISTTTSRIKLVTQLGAVSVSNPTDGSIPEEVKADVYSFGT
                     YLTIQAYSNLVPLGATVLDWESDYRTLASLMVQASNAFAAASGKTKYVLDFEYKKVAP
                     EGHLRIKQIREIPQPSTTPGIVPFLIKEPTPYCIFQGEYGEVFGNHRLKSQLTVETRS
                     MWLTPDNLDRTIYENVFLEYVDSEGNIRTASGSPADWPEASHSYANNTSTDTWVFADM
                     GNPRRYTLTTGRLGVLVAASQSPLLTIRDLGVLSNWGPGYLDLRVDHVLPVPVRTYEG
                     MGTTLTDSVRLFPPFAQEPGESLQTRNLVLNAKISITTQFYWPPTPAGSAAGYTAPLA
                     RWVETRIYGYTSSPIILHSPYSQTYRPEHHNFSEHFLFEPRLEPGISPSILQELHNKN
                     IRVIQVYRPSSGSPIFTTVGFTTPADFDQDNDVDGADYEVFSDCSSGPGLGPVEWACR
                     AADFDHDGDVDQADFGIFQRCISGEKMPPDVDCAQ"
     gene            complement(10641..10883)
                     /locus_tag="P5316_13360"
     CDS             complement(10641..10883)
                     /locus_tag="P5316_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRR85992.1"
                     /translation="MSTLLTKAAAKRVWFDDLNLWIELVDGRQLAVPLAYFPRLLNAT
                     PRQREQFEMSGGGTGIHWDALDEDISFEGLLPGIGD"
     gene            complement(10864..11109)
                     /locus_tag="P5316_13365"
     CDS             complement(10864..11109)
                     /locus_tag="P5316_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRR85993.1"
                     /translation="MPVALRFEGCRFFSFSNEGEPREPAHIHERKGENGAGFRLDQAV
                     TIADSHGTTSSELTRIAQVAAENRDLFLRKRDKYFAD"
     gene            complement(11160..12518)
                     /locus_tag="P5316_13370"
     CDS             complement(11160..12518)
                     /locus_tag="P5316_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR85994.1"
                     /translation="MPDKTCASNASGPCQTRPNTSSSSWSRRRILETAAAGVGALAVG
                     GIRRARAAEASDYGSKVTPSPRSRVLGANDRILLGIIGAGGMGRHHIQSMLDRKENNC
                     EFIAVCDINEANRRAAVEMCGGKAKSFNEHERLLEMKEIDGVLIASPDFWHERHLIDS
                     VMAGKDAYCEKPMSWSIPQGANMVKQVRRTDRIVQIGMQRRSTPSIIEAKRIVDSGFL
                     GEVSLVRAEWYWNMPDLPNPEDIKLEGKVDWDRFQLPLPPEKRYPMNPVKYRFWRYFW
                     EYSGGNMTDQGTHLMDVIQWLFNDSKPPLAAQEHGAAYQIKGYETPDTFCAVFEYPKF
                     MATWTLTYTNNWHDAWSIIAHGRKGTLELDDRGARFYKEKWPANWEDIPPEPEHEISS
                     PLRVDLHEANWLECMRTRKEPNAPVEIGHQAVSALHLANAAHHAKKRATLDPDGLTVR
                     IP"
     gene            12656..13792
                     /gene="waaF"
                     /locus_tag="P5316_13375"
     CDS             12656..13792
                     /gene="waaF"
                     /locus_tag="P5316_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HRR85995.1"
                     /translation="MSNDQPINYSQQTGDSHEVSTQKAPSRILIVLPNWVGDLVLATP
                     ALRVMRERFRDTHIAFLLRSHLEDILSGGQWADEIIHWPATTKGQSRPKRRQGFLGFA
                     AEMRDRRFDWAVLFSNSFRAALLARLAGIRSRIGYDRDGRGLLLTDRLLPERYNGKYV
                     PIPMTRYYNAIARYLGCRNCSDHPELYTTPEEEAVVDGLFAAAGVTQGRPVVVLNPGS
                     SFGPAKRWLPERFAEVADRLVVEHGAAMFIACGPKEIDTARQVAGHMRHSSTVLDKPV
                     LPLGPTKALIRRAGLLITNDTGPRHFGIAFRVPTVTIFGPTHQQWTATNSSCERAIQV
                     PVDCGPCMKRKCPLDHRCMRRVTGDMVVQAARELLSRRTQPVAT"
     gene            13915..14394
                     /locus_tag="P5316_13380"
     CDS             13915..14394
                     /locus_tag="P5316_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRR85996.1"
                     /translation="MARIITDRQELKREVDKLRAAGKKIVFTNGAFDIIHVGHVRYLY
                     GAAAEGDVLVVGVNTDESIKQYKSKDRPLQPLDARMEIVAAFKPVSIVTCFGEPTCDA
                     LLEFIRPDVMCKGPEYGPHNLPEYPTVQRLGIRLATVGGPKENSSTELIRKLQEHGL"
BASE COUNT         2721 a         4357 c         4678 g         2791 t
ORIGIN      
        1 gttccgagaa acggggcgga agcggagcgg ggacggtgag gcgatgaggg gtgcgcgaca
       61 gtctgggcgg gcgtgccgac cggttgcccg ccaaaggcgg gctgataggc cggccgttat
      121 acggctggtt ggtggtggcc cgcggttctt cttcccaagg cggccttcgg ccgcaaccac
      181 gaagggccgc ccgtttacga tcataagccc ttgtttgcag gctagttaca cgttgtagcc
      241 ggcaagatgc gcacaaaacg cacatcagtc gcgacggaag ggactaatgc agctctggcc
      301 ccagcttgcg ctctactgaa tgcgccctga ccccaaatcc tcctgacctc ggactctcga
      361 ggttccgtgt tttcctattg gtggttgccg tgatcgactc gccttcctgc aagatacagc
      421 atgaaacctg cgccggcgag catcaggacc agcgcgtatg tgacaccgac ctcgatgcca
      481 aagcccgtcc ataaccagcc cacaacaacg ctgctcacca aatcgccaat gcccgtcacg
      541 accgcaagcg ccccgaagcc gctccccctg atgccggaag gcagaagctc cgctgcgacg
      601 gcatcctcaa cggcttcata caggccgaca ccggtgcctc cgagtgcaaa catgattgcc
      661 aggcccgcat agctccggac gcccgccacc atgcaagcgg ccgcgagcgc tgcggccatg
      721 taaccggcca ccaggagcaa ccgcttgttc gtacggtccg ccatccatcc gcccagatac
      781 gaccacgagg cgtacaacac gttatgaagc gtgtagccgg caaccgagag tgtcgcggcc
      841 cggcccgggc cgatatcggg cgagagaacg gtcaccgcgt acaggatgta gaaagtgtcc
      901 gcgaaatcac cgagaccgaa taggccgacg gcgccaagga actccttgaa ccgcgaattg
      961 aaaccgccga agctctgaag gaaaggtgtt cggatcggcg ttcggtcctt gcgctcacgc
     1021 accaggagcg tgatggcaaa gacggccaga accgcgggaa tggttgagag caagatcaga
     1081 ccgcggtgag tcaggccaac ccgcaacaag agcagcaccg cgattggcgc gatgatcgcc
     1141 ccggttgtat ccatggcgcg ctcgaacccg aaggcccggc cataggtggt cggcgtaacc
     1201 gcgtcggcaa gcagcgcttt cctggggggc gtgcgcacgc cgcgagacac ccacgccagc
     1261 acacggccac cgagcacaat gaaccagttt gtggccaggg caatgaccac cggcgagaag
     1321 gccatgaaac cgtaaccgcc ggcgcaaagg accttccggc ggcgcagccg atccgccagc
     1381 cagccgccgt agagcttcgc gatgctcgaa aaaccatcgg caaagccctc gatcgtaccc
     1441 aacgccccgg ccgcggcacc catggatgcc aggtaagccg gaagcgcggc cgtcacggtc
     1501 tcgtggctca tgtcgctgaa gagcgaggcc atgccaattc cgaaaaccgt cggtgtgagc
     1561 caacgtgcat ggttcggcgt ggcgccagag gcaggagcgc agtcgttggg tgattccgat
     1621 gacactgaca ggcagcccta tataaactcg gccatcgatt ctatcgctgc cagcagaatt
     1681 gtggtaacgg cgatcagtgc ggcacccccg ttccgcagcg cggtcgccaa tccaaactgc
     1741 cctcgacatg gacgacccac cggcagaatc aaaggcgttt cgttcgcgta tctgtggtag
     1801 gcatcgccgt atcgttgggc caggcggatt tcttcccatg cgatagtcaa accatagaac
     1861 aaaatgaaat acgcgctggc gatcagaaac agcttggaat cgttcaacaa gcagcagaat
     1921 ccatacgcca tcaataccga cccgagataa agaggatggc ggcagagcga gtagaccccg
     1981 gtagtggcaa gctcctcctt cttgcgcaga ctaccgagcg ctgcgaatcg gaaagcaacc
     2041 cccgcaagga ccatcgcaag ccccacgaac acccaggcgt tgggcctgtc cagatcgaag
     2101 gggacctcat ggttgtagac gccctcgccc aggccaaacc caaccagcaa aagtcctgcc
     2161 ttggtccgga gccgcttggt tccgacgatc agggcgaacc aacgtgggtg gtttcccgcc
     2221 cattgaatcc aatcaggtct catcagtcac ccctaatcct ttcattgcag acagtcgtta
     2281 caatcgtccg gccttctcgc ccgatggcta cgccccgcag ggtcaaaggc gcaatggtac
     2341 cggtcctcgt gcgtttgcgg tagtatatac aagatgggca gacccgtcat accagcttgc
     2401 gcgcggagct atcgggcgcg ggttttgaac tcgacgggca gcgaactcag gggcgctccc
     2461 ctctcgctgc gaaaagagtt gaacctgccc ctgttgagcc agaagcggta ggtccatgac
     2521 ggtttgagct taaccgggat cgtcagcacc ctgcgatcct ggtcgtagga aatatcgccg
     2581 gtgatttcgg ggaaattcga gccgccgccg accacggacc agctcttgtc aaccatcggt
     2641 tcgctgaatg tgatcttgat ggccgtcaga tccggatcga cgtcagtggc cccattggcg
     2701 gggactatgg atatgaccct cggtcgttcc tggtccttga ggcctgcggc atagcggttg
     2761 aagaaatcga cgacccgggg catgaaggcc tcaagcgtcg gataccggtc gcgacgacct
     2821 tcgtattccg cgaggagcct gctgagatcg ctgacccagg tgaaaccatg atctgcctcc
     2881 tgccttgccg cggtcgccgc ggctgccttt cccatcgtgt cggcgacgta cctgaccgta
     2941 caggcccgga ccatcgattc ccggagcaga gtcgacgcgt tgccgtaggc ctgccgtctc
     3001 atctcgtctt cgcaggtgct gaagattcgc tgtgcagcgt cgagcatcgt gtccaggtgc
     3061 ttgtccacca ggggattggc gtaggaatga gcgaattcat ggaccgcggt cagggcggtg
     3121 ttgctgtcaa attccggcag tccttcgtgg tcgaacttgt acgcgccgat gatcgagtac
     3181 atctcgacgg gtcccgcggc cgggcggaag ctcggtccgt agcagccacc tccgttgagc
     3241 aggccgacga ccggccgcaa ctgcacctcg gcggttgtcc cgaagtatga atcgaaccat
     3301 tgtcgcaaat cgcggttgcc gagtgccgca tcgaggcggg cggcggccga ggcgtagaag
     3361 tcgcgatgtt gctcaacgaa cgctgcgaag cgggcgtgct cgacaaacag gcgggcctgt
     3421 ttcaggaagt cgcgggcttc ctccgttttc cagcgagggt cgagcatgat cgcatccggc
     3481 ggctggtcga acggcacccg ttccttcagt tcaacggtgt cgctcacatg cacggccatg
     3541 ctcatgaccg cgtcatagct gacgccgcgg ctcgcgcgga gtcgctgtgc ggcaagaacg
     3601 acagggtgat tccggtactt gctgaaatgc ttcttgactt cgtcggcata gaccgactgg
     3661 gagttcggca tgttgtactc cggattgccg gcaaggcgga aaatgatgct catcagctcc
     3721 acgcgggggt cgacgaccgc gcggatcgcc ggtctgccgg cgggtcggct ggcaggcggc
     3781 tcggccgagc tcaggccgac ggccggcgga ctctgagcgg gcttggcgac gcgttgctct
     3841 tgttcgcccg ccaccggggc cccggccaga ccgttcgtcg tcatcagaag agcaaacgcg
     3901 ccgctcaaca ggttcatgat catggttgat tgccccgctc ttgaatatca acgcgatctg
     3961 tctggtgccg ccctcagtcg gtccagatcc atccttatag acgtccaggc gggtcagatg
     4021 ttacacgtgc agtcgcgccg gccgggcagt tcatgagctt tccggggttt gaacccggct
     4081 cagggcgggc atatcatgat tggttcggct cgcggccaga gagcgttgcc aggggggtgc
     4141 cagagtgtcc gagttcgtca ttcatagtga gtttgagcct gcaggggacc agccggcggc
     4201 catcgagaag ctgacgggcg ggtttcagtc cgggcgcaag ttccagtgtc tgctgggggt
     4261 gaccggatcg ggcaagacct tcagcatggc tcacctgatt cgcaacctca accggccgac
     4321 tctggtgatc agccacaaca agacgctggc cgcccagctt tatgacgagt tcagcagcct
     4381 gtttcccgag aacgcagtgg agtacttcgt cagctactat gactactacc agccggaggc
     4441 gtatatcccg cagcgggaca tctacattga gaaggatgcc tcgcgcaacg acgaccttga
     4501 ccggctgcgt ctttcggcga ccagctcgct ggtctcccgc cgcgatgtga tcatcgtcgc
     4561 cagcgtctcg tgcatcttcg gcctaggctc gcccgaagac ttcaaacgga gcatcatcac
     4621 gatccgccgg ggcgaccggg tcgaacgtga cgagctgctg cggcggttcg tggatctcca
     4681 gtacgagcgg aacgatgttg atttccggcg cggtaagttc cgtgtccgcg gcgacgtggt
     4741 cgagctgtgt ccgggcaacg aggaattcgc ctatcgtatc gaactgttcg gcgacgaagt
     4801 cgaccgtatt gacctgatcg acccgctgac caatgagacg atcaccgatc aggacgtgat
     4861 cttcatttac ccggcggtcc actacgtgat gcccgaggac cggatcgcgg cggcgatcaa
     4921 gagcatccag gaggagctcg accagcggct gatggagctc aggcggatgg gcaagttgct
     4981 cgaggcccag cgcctcagcg cccgcacgcg gtatgatttg gagatgatcc aggaagtggg
     5041 ctattgcagc ggcatcgaga actactcgag gcatctgaac ggcacgccgc cgggggccaa
     5101 acctttcacg ttggtggact attttccgaa ggatttcctg ttgatcctcg acgagtccca
     5161 cgtgaccatc ccgcagattc gggcgatgta tgccggtgac cgcagtcgca aggaggtgct
     5221 ggtcgagcat ggttttcggc tgccgtcgtg cctggacaac cggcccatgc ggtttgagga
     5281 gtttgagagc atgtggaacc aggtgctgtt tgtcagcgcc acgcccgggc cgtacgaact
     5341 ggaaaagtgc ggcggcgagg tggtcgagca gatcatccgg ccgacgggac tggtggatcc
     5401 gctgatccac gtgcggccgg ctcgcgggca ggtgcccgat ctgcttgacc gcatcagcga
     5461 gcgagtggcc gtcggcgaac gcgtgctggt aacgacgctg accaagcggc tggccgagga
     5521 cctttcggcc tacgtcgatc agaaaggatt ccgatgcaag tacctgcaca gcgagatcga
     5581 cacgcttgaa cgggtcgaga ttctgaatga tcttcggcgg ggtgatttcg acgtgctggt
     5641 cggcgtcaac ctattgcgcg aaggacttga tctgcccgag gtgtcgctcg tggcaatcat
     5701 ggacgccgac aaggagggct ttcttcggag tgagacgtca ttgattcaga cgatcggccg
     5761 caccgcccgg aacgtcaacg ccgaggtgat cctctacgcc gacaagacca cgccgagcat
     5821 gcaacgggcc atcgacgaga ccaaccgccg gcgcgaaatc cagctcaagt acaacgaaga
     5881 gcacaagatc actcccgaaa cgatcaagaa ggccatccgc agctcgctgg agcaggagat
     5941 ttcggcccgc aagatcgctc gggaggctgt gcatgccgcg gaagatgagt atgatcgcag
     6001 cgagttgatt gcggcgctgg aacaggaaat gctcgaggcg gccgagaggc tggatttcga
     6061 gagggcggcg gtgctgcgag atcggatccg gcagttggag accggaccac aggctcagcg
     6121 agccgacgcc ggacgatagc cacggccagg agaccccgtt tcttggcgag ccggtgctgc
     6181 ttcgcgtggt cttcggtaat accggcggca taggaaccta tcccggcagg tgtgggagcg
     6241 gcatcctggc ggtccgatga gaggccggaa acctgtttca cacagcctgt cgagaccggc
     6301 tgtgaggtca ccggccggtc aagaagaaac gaccagatgg gggatgtttg cgagaggatg
     6361 accatggctc agatgattga tcgtgaacgg cttcggaggc agtgggaaga tgccttggcc
     6421 gaggacggct tcgaccggga catcacctct ttgacgaccg tcgacgacgc agccacgggc
     6481 acggctcgtc ttgtagcccg ccagggcggc gtctttgccg gcgcggtgat cttcgacctg
     6541 ctgcgtgagg cgtactctca caaattgacg gtcacggtca acgcgcagga cggtgcaaac
     6601 atgaggaagg gcgagggaat cgccgtgctg gccggtccga tgcgtctgct cctgggcatt
     6661 gagcgaacct tgctaaactt tctgcagcgg ttgtgcggcg tggcctcgct gacccgtcgt
     6721 tatgtcgatg ccgtcggcgg gacgcaggcg aggatctatg atacgcgtaa gacgattccc
     6781 ggctggcgtc aactggacaa atatgccgtc cgctgcggcg gcggctggaa ccaccgcatg
     6841 ggcctgcatg acgccatttt ggttaaggac aatcacttgg cgggcgtgag tattgattca
     6901 ctggccgtcg ccctgttcga tatggttcgg gaggccgctg cgctggcgcc gccgccggcg
     6961 ttcattgagg tcgaggtcga caccatcgag caactcgagg aagtgttcaa ggtttcgggc
     7021 gtagatgtca ttctgctcga caatttctct attgcccaga tgcgacaggc ggtgaggcgg
     7081 cgggatgaat tgggcttggc cggccgggtg cagtttgagg cctccggcgg tgtcgacctg
     7141 gattcggtcc gcggcatcgc ggaaaccggt gttgaacgga tcgccgtggg agcactcacc
     7201 cactcgtccg gcgcgctgga tatcgccatg gatcttgaga ccaccaggct gtccggctga
     7261 tcagccccgg caaaagcggc cgtcgacgcc ggacagcggc cggtcattgc gcgcaatcaa
     7321 catccggcgg catcttctcg ccgctgatgc atcgctgaaa gattccaaag tcggcctggt
     7381 caacgtcgcc gtcgtggtcg aaatccgcgg cacggcaggc ccactcgacc ggaccgagac
     7441 ccggaccgga gctgcaatca ctgaatacct catagtccgc cccgtcgacg tcgttgtcct
     7501 ggtcgaagtc ggccggggtg gtgaagccga cagtggtgaa gatcggggat ccgctgctgg
     7561 gtcgatagac ttggatcacc cgaatgttct tgttgtggag ctcctgaagg atcgatggcg
     7621 agatgcccgg ctcgagccgt ggctcgaaga gaaagtgctc gctgaagttg tgatgctccg
     7681 gccggtaggt ctgcgagtac gggctgtgca ggatgatcgg ggagctggta tagccgtaga
     7741 tgcgggtctc aacccagcga gccagcggcg cggtgtatcc tgccgcgctg cccgccggcg
     7801 taggtggcca gtagaactgg gtcgtgatgg agatcttcgc gttgaggacc aggttgcgag
     7861 tttgcagaga ctcgccgggt tcctgggcga acggcgggaa gagccgcacg ctgtcggtca
     7921 gggtggtgcc catgccctcg tacgtcctca cgggaaccgg caggacgtgg tcaacgcgga
     7981 ggtccaggta gccgggtccc cagttggaaa gtacgcccag gtcgcggatc gttagcagag
     8041 ggctttgcga agcggccacg agcacgccga gccggccggt ggtcagcgtg taccgccgcg
     8101 gattgcccat gtcggcgaaa acccaggtgt cggtcgaggt gttgttggcg taggaatgag
     8161 acgcctccgg ccagtcggcc ggcgacccgc ttgccgtgcg gatgtttccc tcgctgtcga
     8221 cgtattcgag gaacacgttt tcgtagatgg tccggtcgag attatccggt gtcagccaca
     8281 tgcttctcgt ctcgacggtc aactgcgact tgagccggtg gttaccgaag acctctccgt
     8341 actcaccctg gaaaatgcag taaggggtgg gctccttgat gaggaagggg acgatgccgg
     8401 gcgtggtact cggctgtggg atctcgcgga tctgcttgat ccggaggtgg ccctcggggg
     8461 cgaccttctt gtattcgaag tcgagcacgt acttcgtctt gccggaggcg gccgcaaagg
     8521 cgttggaggc ctggaccatc agactggcaa gagtgcggta gtccgattcc caatccaaca
     8581 ccgtggcgcc cagcggcacc aggttcgaat aggcctggat ggtcaggtat gtgccgaagg
     8641 aatagacgtc ggccttcacc tcttcaggaa tggatccgtc ggtggggttg ctcaccgaaa
     8701 cggcacccag ttgcgtcacg agcttgatgc ggctcgtggt ggtgctgatt ctctcaagcg
     8761 tggccacccc gttggccatc tcgatcgggt cgggcgtcga gtgatggacc aacatggcca
     8821 tgccggcctg gctctcgtcg atgccgtggc ggagtctctc gagataggcg ttgtcgtagt
     8881 agaagctggc gaagaccctg cgaatcgccc ggaacacgcc gcgttcgttc gcctccgcgg
     8941 ggtcgcagag gcttggtccg accccatcgc cgtcgaggtc gtcggccaga cagccgctga
     9001 agctgtcgta caggcccgca ccggtgaact gctcgctgtc ctccacgttg gtcgagctgc
     9061 ggaaccggat tttcttgtac ggatcgaaac cgtatgccgg gtcttgcagg gcgctgatga
     9121 tctcgttttg cagggcgggg ctgaagacgg tggcggaggt cttcttgaag aggtcttcgc
     9181 ggatgccctc aaggacatcg gccagggcgg ccatgttcgc cggcggataa gtgaaaccgg
     9241 ccagacgata ggcgatttct tcgcgaagcg tgcggccgtt ggtcagcgtc tggctgagga
     9301 attcgttcca gaggtcgaac gagaaggcca tggccaccgg gcagttcgac gggatcgacc
     9361 gccgcaacag gccgaagttg gccgctttgc cgccgaagta gcaaatatcc gacggccaca
     9421 gcccatcggt tgaggcgctg tatgcgccat agggcgtgac ggggcagatg ttcagcggcg
     9481 ggggtgtctt gagagcgagg atttcgtcga tctgctgcgg ggtgagcacg tcctgcacgt
     9541 cgatcagccg agcgtcgcag tcattggcgt tcccgtagac ccgcagcgcg atcttgcgac
     9601 cgaccagttg ctgggcccgg gtcgcatcct cggacagcgc caggaaaacg aacggaatgc
     9661 cataggtgtt ggccagaatg gcgacgtgcg aattcggcgt cgaaggggcc agcgtgatga
     9721 tgcccgcgat gaccggcatg tcggcaggca cgccgtcggt caggaggatg tcgccgggtg
     9781 ccagttggcc cgtgagatag gcgttctcga tctgggtggc cgcgaactgt ttgagggttc
     9841 ccagggccca gccgcgcgag tagctgatgt tgcccttagc ccatcggctg ggcgaactga
     9901 cgatgatgcc gttggcctca aagtaggcac ggttttcctc ggccgatgcg atttggtcgt
     9961 agctggggaa atagaaagcc tcgacgccgg cttccgcggt gatctttgac ttgaccaggt
    10021 tgaacaggtt gaccacctgg gccgggtcgt acgcgtctcg gcgaacaagc tcgatgccgt
    10081 attccttgat ttgctcggac ggcggctgat gaacggtcca gtagggcagt atcaccgaac
    10141 ccagaatcgc ctcctgcccc tcggcgtaga gactgatctg gttgaactcc tcggtgctca
    10201 tcccgatgta ttcctggacg aaggtcgttg cccagtcata gtggaacggg taggcgttgc
    10261 tgtcctggaa gtacactttg gtcggatcgc tgacgaagat caggaacttg atccagcccg
    10321 gatcgccggt ttcgggcgcg gcctttcgga aggagtcgct cgggaaggta atggtgtttc
    10381 gccaagagct ggacgggatg accgtctcgg caaaggtgta ccgggtgatg aatccggccg
    10441 ccgataggac catcaggcag gcgagatacc gataaggaaa gcctccgtaa gttcgtaaca
    10501 tggcttgccc tcctgtcttt tccaatcaga gccccccgac ctctattatt gtaccgaatg
    10561 ggaagtaatg gtcaaaggag tcttgggccg tcggaggggc cttggcagga ttagtggtct
    10621 gccgatgccc ggcgtctcgt tcaatccccg atccccggca acaggccctc gaagctgatg
    10681 tcttcgtcga gcgcatccca gtgaataccg gtgccccctc cgctcatttc gaactgctcg
    10741 cgttgccgcg gtgtagcgtt cagcagccgg gggaaatagg ccaacgggac cgccagttgc
    10801 cgcccgtcga ccaactcgat ccacaggttg agatcgtcaa accagacgcg tttggctgcc
    10861 gccttagtca gcaaagtact tatcccgttt cctcaagaat aggtcgcggt tttccgcagc
    10921 cacttgggca atccgggtca actcgctcga agtcgtgccg tgcgagtccg cgatcgtcac
    10981 tgcctggtcc agccggaatc cggccccgtt ttcgcccttg cgttcgtgga tgtgggcagg
    11041 ttcgcgcggt tcgccttcgt tcgaaaagga gaagaacctg cagccctcga aacgcaaagc
    11101 aacaggcact cgacaactcg caaccggcct cccaggcccc cgatccctgt cctgccgcat
    11161 caggggatgc ggacggtcag accgtcgggg tcgagggtcg cccgcttctt ggcgtggtgg
    11221 gcggcgttgg ccagatgcag ggcggagacc gcctgatggc cgatctcgac cggagcgttt
    11281 ggctccttac gggttcgcat gcactcgagc cagttggcct catgcaggtc gacccgcagc
    11341 gggctggaga tctcatgctc aggctctggc ggaatgtcct cccagttggc gggccacttt
    11401 tccttgtaga accgcgcccc ccgatcgtcg agttccagtg tgcccttgcg gccgtgggcg
    11461 atgatcgacc acgcatcgtg ccagttgttg gtgtaggtca gggtccaggt cgccatgaac
    11521 ttcgggtact cgaagaccgc gcagaacgtg tccggcgtct cgtagccctt gatctggtac
    11581 gccgcgccgt gctcctgggc ggccagtggc ggcttgctgt cgttgaatag ccactggatc
    11641 acgtccatca gatgagtgcc ctggtcggtc atgttgccgc ccgagtactc ccagaagtac
    11701 cgccagaacc ggtacttcac cgggttcatc gggtaacgtt tttccggcgg cagcgggagt
    11761 tgaaaccggt cccagtcgac cttgccttca agcttgatgt cctccgggtt gggcaagtcc
    11821 ggcatgttcc agtaccactc ggctcgcacc agactgacct cgcccagaaa gccgctgtcg
    11881 acgattcgct ttgcctcaat gatagatggg gtgctgcgtc gctgcatgcc gatctgcacg
    11941 atccggtcgg tgcggcggac ctgcttgacc atgttagccc cctgcgggat cgaccaggac
    12001 atgggttttt cgcagtaggc gtccttgccg gccatgaccg agtcgatcag gtgtcgctca
    12061 tgccagaaat ccgggctggc gatcagcacg ccgtcgatct ccttcatctc cagcagccgc
    12121 tcgtgctcgt tgaagctctt ggccttgccg ccgcacatct cgacggcggc cctgcggttg
    12181 gcctcgttga tgtcgcagac ggcgatgaac tcgcagttgt tttccttgcg gtcgagcatg
    12241 ctctggatgt ggtggcgacc catcccgccg gcgccgatga tgcccagcag aatgcggtca
    12301 ttggccccga ggacccgtga tcgggggctt ggtgttacct ttgagccgta gtccgaggct
    12361 tcggcggccc tggcccggcg gatgccgccg acggccaagg cgccgactcc tgcggccgca
    12421 gtctcgagga ttctgcggcg gctccagctc gacgacgatg tgtttggtcg tgtttggcat
    12481 ggaccagaag cgttgctggc gcaggtcttg tccggcatct aagtctcctt ccgttacgat
    12541 cttgaaagct gcatttccaa agccgcggca ggcccacagg ccatcgcgac gccgtaaggg
    12601 taccgagcca agggggagtt gtcacgtgag ggagggttgt catacaattc ccggcatgtc
    12661 caacgaccaa ccgataaact acagccaaca gaccggcgat tcccatgaag tgtcgacgca
    12721 aaaagccccg tcgcggatcc tgatcgtcct gccgaactgg gttggtgatt tggtgctggc
    12781 aaccccggct ttgcgagtca tgcgggagcg gttccgggat acacacattg ccttcctgct
    12841 tcgcagtcat ctggaggaca tcctcagcgg ggggcagtgg gctgacgaga tcatccactg
    12901 gccggccaca accaaaggac agagccgtcc caagcggcgg cagggcttcc ttggttttgc
    12961 ggccgagatg cgggatcggc gatttgactg ggccgtgctg ttctcgaatt ccttccgggc
    13021 ggcgctcctg gcacgcctgg cggggattcg ttcacggatc ggctatgacc gcgacggcag
    13081 ggggctgctg cttaccgaca gactgctccc cgagcggtac aacgggaaat acgtgccgat
    13141 cccgatgacc cggtactaca acgcgattgc ccggtatctc ggatgccgca actgctctga
    13201 tcaccccgag ttgtacacga cgcctgagga agaggccgta gtggacgggt tgtttgccgc
    13261 tgccggcgtc acgcagggaa ggcccgtcgt cgtgctcaac ccgggctcct ctttcgggcc
    13321 ggccaagcgg tggcttcccg aacgatttgc cgaggtggcc gaccgccttg tcgtggagca
    13381 tggtgcggcc atgtttatcg cctgcggtcc aaaggagatc gataccgcac ggcaggttgc
    13441 cggccatatg cgtcattcca gcaccgtcct ggacaagccc gtgttgcctc tcggcccgac
    13501 caaggccctg attcgacggg ccggcctgct gattaccaac gacaccggcc cgcgacactt
    13561 cggtattgcc ttccgagtgc cgacggtgac catcttcggt ccgacacacc agcagtggac
    13621 cgccaccaac agttcgtgcg agcgggcgat ccaggtgccg gtggattgcg gtccatgcat
    13681 gaagcgcaag tgcccgctcg atcaccgctg tatgcgccgc gtaaccggcg acatggtcgt
    13741 gcaagccgct cgcgagttgt tgagcaggcg aacccagccg gttgccacgt gagattcgcg
    13801 ggtgtcaccg gccgcttgcc cgccggcgtc ttctcgctcc gaacctcgcc ggagaaatgc
    13861 ccgccggaac aatccgcctg gtgcgaggtt cgattcagtt gtacaggaga cccgatggct
    13921 cgcattatca ccgatcgcca ggaactcaag cgcgaggtgg ataagcttcg cgccgccggc
    13981 aagaagatcg ttttcacgaa cggggcgttt gacatcatcc acgttgggca cgtgcgatat
    14041 ctttacggtg ccgctgccga aggcgatgtg ctcgttgtcg gcgtgaacac ggacgagtcg
    14101 attaagcagt acaagagcaa ggaccggccg ctccagcccc tcgatgcccg gatggagatc
    14161 gtcgccgcct tcaagccggt cagcatcgtt acctgcttcg gcgagccgac ctgcgacgcc
    14221 ctgttggagt tcatccgccc ggacgtcatg tgcaagggcc ccgagtacgg cccgcacaac
    14281 ctcccagagt atcccaccgt gcagcgcctc ggcattcgcc tggccacggt cggcggcccg
    14341 aaggaaaact catccaccga gctgatccgc aagttgcagg agcatgggtt gtgagagctt
    14401 gagaacctta cggccagaca tctgcgcggc tcgcgcgcat gtttcccgat cgccgtgcgc
    14461 ttggctcgcg gcccgcaaaa aggcgctccg ccttagattt cgacattcgt cattcggccc
    14521 tccgccgggt gccatgccca cggcttt
//
LOCUS       DAOTPW010000142        14490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4070_length_14490_cov_5.403810, whole genome shotgun sequence.
ACCESSION   DAOTPW010000142 DAOTPW010000000
VERSION     DAOTPW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14490
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_14490_cov_5.403810"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="P5316_13385"
     CDS             <1..1488
                     /locus_tag="P5316_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HRR85997.1"
                     /translation="LSCDVPQTGTFSVGNFTAPIGEDQGAWGPFRHNYRLDFTPLAKS
                     GRYKVRFGDTASLPFTVGHDAYADVPDKLLGFMRLQRCGDNPVTGKKCHQQDAIDTTD
                     GRKVDLVGGWHDAADRLKHMITTTYCVAALFLAGAEEEARYGAVLVGKLQPDPDTIYV
                     QIGDDRDHMPPATLWHDDQSDYGWGKGGPRAAWRATGRPEGPKYKNNSSGMANLAGRA
                     SAAMTLAGNLKTAQSMYDLARRRPGNAMSVPVRAPHYYGESTYHDDLEWAATELYVAT
                     KDPQYLKQAVQYAGQAGPNPWMGKTRHGHYEFFPYVNLAHWRLYPYVDEETKKMLADH
                     YRVGLEAVRRIAERNPYRLGTPLVWCSTNDVIALATQAVLYEKMTGDRTYRTLAAEAR
                     DWIFGRNPWGVSMVIGVPQDGNPVRRPHHRFHSMAGILPVGGLVDGPVYKDINDSLKF
                     EPFGNDLYARFQSEIGVYHDCYEDFSTNEPIIDGTVSLLLLLKVW"
     gene            1490..2683
                     /locus_tag="P5316_13390"
     CDS             1490..2683
                     /locus_tag="P5316_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR85998.1"
                     /translation="MGRLRIIGLVREANYVRQMCLSPMTAAQRQNLRNRVQRTLSQIS
                     AICDRHGVRPGQLPGPSRRAYEFLLKLDVDNLPTVETPPQQHLPQKHLPDSVRLVGLR
                     AFLDTLLDDLARQAHLGRLDAAAMLRVVQQTAQRLDHHMSRNEFEPGHLRTESRDLVA
                     WFRYFARPENMDRYVQAVRRAQAVFGAMPEANDRWRAPLLVHFRPSGHLYRWRVGARG
                     TRMVLSTPMICFDDVTFGHLGRMMLGNRQHLPAVNAAMLSHPYQSIRTAMDEAAGRVE
                     HTKGMTYDLAEVFDDVNRRYFGGSMSRPKLSWTRRLTGRRFGHYNFAHDVVSISSTLD
                     RPDVPRFVIEHVMHHELLHKKHGSRWNGSQRRCHTPQFRVEERTFEQFQEADKFLNAL
                     SRRMH"
     gene            2773..3567
                     /locus_tag="P5316_13395"
     CDS             2773..3567
                     /locus_tag="P5316_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HRR85999.1"
                     /translation="MKTKLGPLETRLLAYAQMRRLQVVRGGELAEPLHLSAQQEMRLL
                     SRLVRAGIIARVRRGLYLLPPRLPLGGKWSPSEVLALSTLMRDRQGRYQICGPSAFSR
                     YGFEQQIPTRTYAYNNRLSGDRRIGATSFTLIKVADERLGSTEEVPVDEGLTAVYSSR
                     VRTLVDAVYDWSRFDSLPQGYDWIRRELASRRVGAKQLVAVTLRYGNQATLRRVGALL
                     EREGISGRLLARLRRALRPSQCLIPWVPSRPKRGRTDQQWGVVFND"
     gene            3560..4450
                     /locus_tag="P5316_13400"
     CDS             3560..4450
                     /locus_tag="P5316_13400"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRR86000.1"
                     /translation="MTEAEQPAIDAHEDPDLFREALNFTAAETFFPERLIEKDYFCTV
                     LLKYLASFGGQLIFKGGTCLAKVHAGFYRLSEDLDFVIPTPLTASRSQRRRRVTALKN
                     AVAGLAERVPCFRVTGQLLGADDSSQYVGTVSYSSLISRRDETITIEVSLREPLLMPT
                     MEGSVRTLLLDPVSGRRMVSPAVVPCIARVEAIAEKYRAALTRRDVAIRDFYDVDYAA
                     RKLSIRPEDTELVGLVRRKLAVPGNGPINVGPDRLAQLRAQLDARLKPVLRRPDADAF
                     DLDRAFGIVREMAERLPVAS"
     gene            complement(4634..4942)
                     /locus_tag="P5316_13405"
     CDS             complement(4634..4942)
                     /locus_tag="P5316_13405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86001.1"
                     /translation="MPRIEQIERMLASSPDDVFLNFALAMEQVKVGRQEQAVAGFRRV
                     IELDPDYVPAYTQLSNALIDLGRKEEAKAVLASGVEAAARAGDKHAAGKMGDKLKLLE
                     "
     gene            5157..5651
                     /locus_tag="P5316_13410"
     CDS             5157..5651
                     /locus_tag="P5316_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005901290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRR86002.1"
                     /translation="MIATVLGGIGPGNRKQGRRHMTEQARKVREIMTTEVVTIGMDET
                     LETARRIFNQRRFHHLIVVEGGKPVGVISDRDLLKNLSPFVGVRMSERAQDIETLRRR
                     IHQVMTRRLVSIEPDAPVAQAARAMMHHKVSCLPVIKNGSTLVGIITLRDLVRWVVID
                     SIEK"
     gene            5753..6409
                     /locus_tag="P5316_13415"
     CDS             5753..6409
                     /locus_tag="P5316_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86003.1"
                     /translation="MSTSPAKRVGIGRSFVAGCCAGIPAGVILAYLAPMPFYLGLFFF
                     LVLGLVIGAIMFRFGRGAVPVRPLTLNLIGSAVVLLTWSTTLVTEYATLPRLVARRTE
                     TSMFRRLTPQQKAEVAAKSRQHVMSRLLGRPFDEGLGDWLAGFPKYLRWIAQDGTMEC
                     PRVVDPTTFTFKAGQSKASWVFRVVVSMALLAFAVLSQYLLLARTTKDQPSIDAIPSK
                     "
     gene            6844..7848
                     /gene="bamD"
                     /locus_tag="P5316_13420"
     CDS             6844..7848
                     /gene="bamD"
                     /locus_tag="P5316_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRR86004.1"
                     /translation="MELRAGEVPVPPKRHAEHLVFNEKTGEWEYGVEPIPGTEDGDLD
                     IARQWLAQGEYKTTCKIVRDWMKQYGSDAPRYPEALYLYGTAYLERGLYRQAHKAYQE
                     LLDNYPGSQYAESALSQEFRIAEQYMAGKRKRIWGGLLWFRDREGGIAIMDDLVANYS
                     DTPLAEMAQMAKAEYYFSRGELDLAESEYATFAREYPRSRWHARALLMSARCAQGRFA
                     GIKFDEAPLIEAEERYRQYLREYPAAAERDEVSTTIQQITATRADKTLDIGKFYEKTE
                     KFQAARFYYREIIIRWPKTPAALEARNRLAAIGEPLEEPAILEPQASTNAVRHQETQR
                     "
     gene            7848..8378
                     /gene="lptE"
                     /locus_tag="P5316_13425"
     CDS             7848..8378
                     /gene="lptE"
                     /locus_tag="P5316_13425"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRR86005.1"
                     /translation="MQPTSHHHRSVAALDIGRLALLAGSLLVCGCGYSNESLHPQTIR
                     TVYVEMFQSREFRRGIEFELTEALRKEINVSTPYTNAPPEKADTILSGEVLEWRESSL
                     GWDPIAIRPRETAATLIVRYRWKDVRTGKLLRDRPRFVTTVTYVRPAGETAAEGRLDA
                     VSKLARNIVGDMENSW"
     gene            8456..10480
                     /gene="ftsH"
                     /locus_tag="P5316_13430"
     CDS             8456..10480
                     /gene="ftsH"
                     /locus_tag="P5316_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRR86006.1"
                     /translation="MSENPSDRDDRQGSRPPGPPMRMSRGLFGWAASIIIFVFLAFYL
                     MSNAGQRRELTVDQFWKELADNNIQELVIQANNNTIRGKLKNTQGKDQPPDFEVSWDK
                     SAFNDAFWTELRASGVRYRIDQTGGALFNILINLLPWLLVIAFAWFFVFRQLRGVGGG
                     PGGMLGNFGRSRHRVTTKEHTNITFADVAGIDEAKEECAEIIEFLKNPKKFQRLGARI
                     PRGVLLVGDPGCGKTLLAKAIAGEADVPFFSISGSDFVEMFVGVGASRVRDLFKQAKD
                     NSPCIIFLDEIDAVGRRRGAGFTSGGHDEREQTLNAILVEMDGFDTSDQVVVIAATNR
                     ADVLDPALVRPGRFDRQVFVPLADVKGRLEILKVHAKKVKLGPDVDLNKLARGTPMFS
                     GADLAAIINEAALLATMANKDYVEMEDLEEARDKVRWGRAKKSRVVDEKERIATAYHE
                     AGHAVAQCLLPDADPLHKVSIIPRGPYGGASFSLPEKDRTTYSSKWLNAQLRILCAGR
                     IAEEITCNDVNTGASADIRQATETARRMVAEWGMSDRVGFIYYGDDPSRKTMWVDLPG
                     GKEYSEETARLIDQEVQRVLSEAYTDTRRLLTENRQKLEAVAQALLKYETLDAAEVHA
                     LLRGESLDRPTVSDLLDAAHAGIGVRASAGPGTSKSSQPEFGSGPLPQPG"
     gene            10524..10985
                     /locus_tag="P5316_13435"
     CDS             10524..10985
                     /locus_tag="P5316_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR86007.1"
                     /translation="MANAEIIPVGMNDLPLIVDLYNEIFRPGREHAFFAHRLGTQRNP
                     LLLLAQVQKRPVGFALGYELKQSTFYCWYIGVLPEFRRAGVASQMMEAMTAWARDQGY
                     QIIRFECYNRHRPMLHVAIRQNYNVVGIRYDADAEDNLIILEQNVNEEAAD"
     gene            11248..12360
                     /locus_tag="P5316_13440"
     CDS             11248..12360
                     /locus_tag="P5316_13440"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRR86008.1"
                     /translation="MADENRIERIGILTGGGDCPGLNAVIRAVAKAAMHQHGLEVWGI
                     EDGFLGLIRNRMRLLGRDDVSNILTRGGTILGTSNTANPSRFAVGIDEQGNPVVQDVT
                     QRVIEHIAERQLDVIVCVGGDGTMSGAASLIQHGVRCVGVPKTIDNDLMHTEITFGFL
                     TAVNTATEALDRIHTTASSHHRVMLVEIMGRNAGWLALTSGLASGADVILIPEIPYSL
                     EAICDYCLNRSKRGKSFTIIAVSEGARPIGGEQTVDRVVAGSPDPIRLGGVSNVLCKQ
                     IEAATRLECRATILGHVQRGGTPVPADRVLGTLFGHKALELALAGKFNEIVVSQNGRI
                     TSVPIESVAGRQRLVPLDHPLIAAARSVGTSFGDGL"
     gene            complement(12447..13886)
                     /locus_tag="P5316_13445"
     CDS             complement(12447..13886)
                     /locus_tag="P5316_13445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR86009.1"
                     /translation="MANRPVVLINSNRMKPAIAPLALDYVAGSLRRGGFEVHLIDLCF
                     ADDPAEALRQGLADAEPLLIGVTFRNTDDCYYPSCAFFVPELAELVRQIPTLTDAPVA
                     LGGCGFSVFPAEIVSICKSDFGIIGDGEEGFLGLARALSGNKDYHTLPGLVWREADGR
                     CMLNPPQPPAVLDVSPDRSLLDNARYFREGGQGNVETKRGCPMSCIYCADPVAKGRQV
                     RCRPPTQVADEVESLLKQGVDVLHLCDGEFNIPYDHALAVCREMIRRGLGQRVRWYVY
                     CSPHPFSDELALAMKQAGCVGVNFGSDSGCDRMLAALGRSYRRDAIRDAVQACKAAGI
                     TVMVDLLLGGPGEDEASVRETIEFVRRLDPDRCGAATGVRLYPGTPLAESIRRQGPLS
                     ANPNLRGCVEDNDSLLRPVFYFDRRLSDDPGSLVCDIIAGDERFFPPPRIRDATNYNY
                     NDNRVLTDAIGRGMRGAYWDILRRLAAEK"
     gene            complement(13969..14385)
                     /locus_tag="P5316_13450"
     CDS             complement(13969..14385)
                     /locus_tag="P5316_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86010.1"
                     /translation="MSTLHISIGLTVIAVLCACVFVVLIIKSRKARGNERRRYAKINL
                     LWNMAFTVFVCAQAIFSFRQSGNGTLGLMIMLPLLVLALACGVFGLRSLRSDKYFGKL
                     YELDPGFCGRCGYNLTGNTSGVCPECGWKLPERKEA"
BASE COUNT         2860 a         4655 c         4400 g         2575 t
ORIGIN      
        1 ctctcatgcg atgtgccgca gacgggtact ttctcggtcg gcaatttcac ggccccgatc
       61 ggcgaggacc aaggcgcgtg gggaccgttt cgtcacaact accgattgga cttcacccca
      121 ctggccaaga gcggccgcta caaggtgcga ttcggcgaca ccgcgtcgct gcccttcacc
      181 gttggccacg acgcctatgc cgatgtgccg gacaagctgc tcggtttcat gcgccttcag
      241 cgctgcggcg acaatccggt caccggtaag aaatgtcatc agcaggacgc catcgacacc
      301 acggacggcc gcaaagtcga cctcgtcggc ggctggcacg acgcagccga ccggctcaag
      361 cacatgatta caacgaccta ttgcgtcgcg gccctgtttc tggccggcgc ggaggaagag
      421 gcccgctatg gggcggtcct cgtcggcaag ctccagcccg atcccgacac gatttatgtt
      481 cagattggtg atgaccgaga ccacatgccg ccggctacat tgtggcacga cgaccagtcc
      541 gactacgggt ggggcaaggg tggtccgcga gccgcctggc gggccaccgg tcgaccggaa
      601 gggccgaagt acaaaaacaa ctcttcaggg atggccaacc tggccggcag ggcttcggca
      661 gcgatgacat tggccggcaa tctcaagacg gcccaatcca tgtacgacct ggcccgccga
      721 cgccccggca acgccatgtc cgtgccggtc cgagctccac actactacgg cgagagtacc
      781 taccacgacg acctggagtg ggccgcgacc gaactgtacg tcgccaccaa agatccccag
      841 tacctcaagc aggcggttca gtacgcagga caggccgggc caaacccgtg gatgggcaaa
      901 acccgccacg gccactatga gttcttccct tacgtcaacc tcgcccactg gcggctctat
      961 ccgtacgtgg atgaggagac gaagaagatg ctcgccgatc attatcgagt cggcctggaa
     1021 gcggtgcgcc ggatcgccga gcgcaaccct taccgcctgg gtactccgct ggtctggtgt
     1081 tcgaccaacg acgtgatcgc cctggctacc caggccgtgc tctatgagaa gatgaccggt
     1141 gaccgcacct accgcacgct ggcggctgag gcccgcgact ggatcttcgg ccgcaacccg
     1201 tggggcgtct cgatggtgat cggggtcccc caagacggga acccggtgcg acgtccgcac
     1261 caccgcttcc atagcatggc cggtattctg cccgttggcg ggctggtcga tggacccgtc
     1321 tacaaggaca tcaacgacag tctgaagttc gagccttttg gaaacgacct ctacgcacgg
     1381 ttccagtccg agatcggcgt ttaccatgac tgctatgagg acttcagtac caacgagccg
     1441 atcatcgacg ggaccgtctc gctgcttctg cttctgaagg tttggtgata tggggcggct
     1501 tcgaatcatt ggtttggtgc gcgaagcaaa ctacgtccgc cagatgtgtt tgtcaccgat
     1561 gaccgccgcc cagcgccaga acctcaggaa tcgtgtgcag cgaacccttt cgcagatcag
     1621 tgcgatctgc gaccggcatg gcgttcggcc tggccagttg cccggaccca gcaggcgagc
     1681 ctatgagttc ctgctcaagc tggacgtcga caacctgccg accgtcgaaa cgccccccca
     1741 acaacacctg ccgcagaagc acctgcccga ctcggtccgc ctcgtcggtc tgcgcgcgtt
     1801 tctcgacacc ctgctcgacg acctggctcg acaagcgcac ctgggccgat tggacgccgc
     1861 cgcgatgctg cgagtggtgc agcagaccgc tcagcgcctg gaccatcaca tgagccgcaa
     1921 cgagttcgag cccgggcatc tgagaaccga gtcccgagac ctggtcgcct ggttccgcta
     1981 cttcgcccgg ccggaaaaca tggatcgcta cgtgcaggcg gtacgccgcg cgcaagccgt
     2041 cttcggggcc atgccggagg caaatgatcg ctggagggcg cccttgctgg tgcacttccg
     2101 gccctcaggt cacctctatc gctggcgagt cggagccagg ggtacgcgga tggttctttc
     2161 aacacccatg atctgctttg atgacgtgac gttcggccat ctggggcgca tgatgctcgg
     2221 caaccggcag catctgccgg ccgtcaacgc ggccatgctg tcgcacccgt accagtcgat
     2281 tcgcacggcc atggatgagg cggccggccg ggtcgagcat acgaagggaa tgacctacga
     2341 cctggcagag gtcttcgacg acgtcaatcg gcgctatttc ggcgggagca tgtcccgccc
     2401 gaagctgagc tggacccgac ggctcaccgg gcggcggttc ggacattaca acttcgccca
     2461 cgacgtggtc agcatcagca gcacgctgga tcggccggac gttccgcggt tcgtcatcga
     2521 acacgtgatg caccacgaac tgctgcacaa aaagcacggc agcaggtgga acggttctca
     2581 acgccgctgc cacacccccc agttccgcgt cgaggaacgc acgttcgagc agtttcagga
     2641 ggccgacaag ttcctgaatg ccctcagccg ccgaatgcac tgagcaagaa gccgcagtct
     2701 gcccatgaac cgcttgtatc ctatttttag ttaatgcctt gacaaggtat taacgagaaa
     2761 tagaatacaa ccatgaaaac gaaactggga ccgttggaga cccgtttgct cgcctacgcc
     2821 cagatgcgac gactccaggt tgtgcgaggg ggcgaactgg ccgagccgct gcacctgtcg
     2881 gcacaacagg aaatgaggct tctgagtcga ttggtccggg cggggataat cgcgcgagtg
     2941 cggcgcggcc tgtacttgtt gccaccgcgg ctgccgttgg ggggaaaatg gagcccgagt
     3001 gaagtccttg ctctcagcac gttgatgcgg gaccgccaag gccgttacca gatttgcggg
     3061 ccgagtgcat tcagccgcta cggtttcgaa cagcagatcc ccacgcggac ctatgcttac
     3121 aacaaccggc tgtcggggga ccgcaggatc ggcgcgacct cgttcacgtt gatcaaggtg
     3181 gcggatgagc gactcggcag caccgaagaa gtcccggtcg acgaaggtct gacggcggta
     3241 tattcctcac gcgtacgaac cctggtggat gcggtctacg actggtcgcg attcgacagc
     3301 ctgccgcaag gatacgactg gattcgtcgc gagttggcaa gtcggcgcgt cggcgccaag
     3361 cagcttgtcg ccgtgacgct gcgctacggc aaccaggcaa ccttgcggcg cgtcggcgct
     3421 ctgctggaac gagaaggcat atccggtcgt ttgctcgcca ggctgcggcg agcgctgagg
     3481 ccgtcccaat gcctgatccc atgggtcccc tcccggccta aacggggcag gacggatcaa
     3541 caatgggggg tagtgttcaa tgactgaggc tgaacaaccc gccattgacg cccatgagga
     3601 ccccgatctg tttcgggaag cacttaactt cacggcggcc gagacgttct ttccggaaag
     3661 gctgatcgag aaggactact tctgtacagt cctcttgaag tatctcgcct ccttcggcgg
     3721 ccaactgata ttcaaaggcg gaacgtgtct ggccaaggtg cacgccggct tctacaggct
     3781 gagcgaagat ctggacttcg tcatcccgac acccctgacg gcatcccgat cgcaacgacg
     3841 aagacgagtg acagccttga agaatgctgt cgcggggttg gccgagcgtg ttccatgttt
     3901 tcgtgtgacg gggcagttgc tcggcgccga cgactcgtcg cagtatgtcg ggaccgtcag
     3961 ctactcctcg ttgataagcc ggcgtgacga aaccatcaca atcgaagtca gtcttcgtga
     4021 gccgctcctg atgcccacga tggaaggctc ggtacggacg ctgctgctgg atccggtgtc
     4081 tggtcgccgg atggtttcac cggctgtggt tccctgtatc gccagggtgg aggcgatcgc
     4141 cgagaagtac cgcgcagcgc taacgcgccg tgatgttgcg attcgcgatt tctacgacgt
     4201 ggactacgct gcgagaaagt tgagcattcg gccggaggac acggaactgg tcgggctggt
     4261 cagacgcaag ctcgctgtgc ccggtaacgg tccgatcaac gtgggtccgg accggctggc
     4321 gcagttgcgc gcgcaactgg atgcccggtt gaaacccgtg ctgcgccggc cggacgccga
     4381 tgcattcgac ttggatcgcg ccttcggaat cgttcgcgaa atggccgaac ggttgcccgt
     4441 agcaagctga ccgaaacgcc cggccaatcg ccgcaccgac gaaaaggcgt tcaagcaatt
     4501 ggtggaggcc gacaagttcc tgaatgccct cagccgccga atgcactgag caagaagccg
     4561 cagtctgcac atgaaccgct tgtatcctgt ttttagttaa tgccttgaca aggtcttaac
     4621 aggaaataga gagctactcg agtagtttca gcttatcgcc catcttgccg gcggcgtgct
     4681 tgtcaccggc gcgagcggcg gcttcgacgc cgcttgccag aacggccttg gcctcttctt
     4741 tgcggccaag gtcgatcagc gcgttgctca actgggtata ggcagggacg taatcggggt
     4801 ccaactcgat gacccgccgg aagcccgcga cggcctgctc ttgccgccca accttgactt
     4861 gctccatggc cagggcgaag ttcaggaaaa cgtcgtccgg actggacgcg agcattctct
     4921 cgatttgttc gattcgcggc acgaaagcaa taccctcagt caacccaaaa acgtgcgaaa
     4981 accgccgcga ccctgcccca gcgcagcggc gaggtcgccg cggcgaccaa aacctgccgc
     5041 attgttgagc tcgggcccgc acgtccccgc cgccttcggc ggctgaccac cccctgacct
     5101 tttcacgccc gttcctgaac gcaaggccgg acctgtcaac cggcccgtgc cacgctatga
     5161 tagctactgt acttggcggc atcgggccgg gcaaccgcaa acagggacgg cggcacatga
     5221 cggaacaagc tcgaaaagtg cgcgagatca tgaccaccga agtcgtcacc atcggcatgg
     5281 acgagacgct ggagaccgcc cggcgaatct tcaatcagcg ccggttccac cacctgatcg
     5341 tggttgaggg cggcaagccc gtgggcgtta tttcggatcg agatctgctg aaaaacctca
     5401 gcccgtttgt cggcgttcgc atgagcgagc gcgcccagga catcgagacg ctgagaagac
     5461 gcatccacca ggtcatgacg cgcagactgg tctcgattga acccgacgcc ccggtcgccc
     5521 aggcggcccg tgccatgatg caccataagg tctcgtgcct gccggttatc aagaacggct
     5581 ccaccctggt cggtatcatc actctgcgcg acctcgtccg atgggtggtg atcgattcga
     5641 tcgaaaagta gatcttccga gagacgcgtg cgaagcaccc agccggacgc agcggtctgt
     5701 cttcgccggg gccgctggca aacaacctgc gcagcggcta gactgccgcc gcatgagcac
     5761 tagccctgca aaacgcgttg ggatcggccg gtcctttgtg gccggttgct gcgcgggcat
     5821 cccggcaggg gtgatacttg cctatctggc cccgatgccg ttttacctcg gcctgttctt
     5881 cttcttggtt ctcgggctgg tgatcggcgc gatcatgttt cgtttcggcc gaggtgccgt
     5941 tccggttcgc ccgctgacgt tgaacctgat cggatcggcg gtcgtgctcc tgacttggag
     6001 caccacgctc gtcaccgagt acgcgaccct cccccgactg gtcgcccggc ggaccgagac
     6061 ctccatgttc cgccgtctga cgccgcagca gaaggctgaa gttgccgcca agagccgtca
     6121 gcacgtcatg agccggctcc tgggacgccc ttttgacgaa ggcctcggcg attggctggc
     6181 cggcttcccg aaatacctcc ggtggatcgc ccaagacggc acaatggaat gcccacgcgt
     6241 agtggacccc acaacgttca ccttcaaagc cggccagagc aaagcttcct gggtctttcg
     6301 cgtagttgtc tcaatggcct tgctggcgtt cgctgttctc tcccagtacc tgctcctggc
     6361 ccggaccaca aaggaccaac cttcaattga cgccattccg tccaagtagc gcaggcacgc
     6421 aagccggctt cctcaggggc aatcgcttgt ccgcgacgtc actcggcgga tggcgcgctg
     6481 tcgaaggcaa gccgtaactg cttgtcacag cgaatctccc ggccgcctga ggaggccgcc
     6541 gcgcaattgc cacggcggca gaagccctct ataatcgccg tctcctggag aatatccggc
     6601 cgagcgggcg gggttatccg cacaggccac gtgaaaccag agccacatgg aaccctggcc
     6661 atgagtcttg tccctccgaa cctgagtctc agtctcttcc gtggggactg gtgcgcattc
     6721 tgcgcgcatg tttctggatc gcagagcgct tgggttgcgg cccgcaaaaa ggcggtccgc
     6781 cttagctttc ttgcccgtcc cggcgtttgt ctgctggctc tggcggttgc cggcgcttgc
     6841 cccatggaac tacgggccgg cgaggtgccc gttccgccga aacggcatgc agagcacctt
     6901 gttttcaacg agaagaccgg agaatgggaa tacggcgttg agccgattcc cggaactgag
     6961 gacggcgacc tggacatcgc ccgccagtgg ctggctcagg gtgaatataa gacaacctgc
     7021 aagatcgtca gggactggat gaagcaatac ggcagtgatg ccccgcgata ccctgaggca
     7081 ctgtacctct atggcactgc gtacctggag cgaggcctgt accggcaggc ccacaaggcc
     7141 taccaggaac tgctcgacaa ctatccgggt tcgcagtacg ccgagtcggc gctctcccag
     7201 gagttccgca tcgccgagca atacatggcc ggcaagcgaa agcgcatctg gggcggtctg
     7261 ttgtggttca gggaccgcga gggcggcatc gcgatcatgg atgacttggt tgccaactac
     7321 tccgacaccc ccttggcgga aatggcccag atggccaagg ccgagtatta cttcagtcgc
     7381 ggcgagctcg atctcgccga atccgagtac gccacattcg cacgcgagta tccccgcagc
     7441 cgctggcatg cccgagccct gctgatgagc gcccgatgcg cccaaggccg cttcgcgggt
     7501 atcaagttcg acgaggcccc attgatcgag gcggaagagc gctaccgtca gtacctgcgg
     7561 gaatacccgg cggccgccga gcgcgatgag gtgtcgacga cgatccaaca gattacggcc
     7621 actcgggcgg acaaaacgct cgacatcggt aagttctacg agaaaaccga aaagtttcag
     7681 gccgcgaggt tctactaccg ggaaatcatc atccgctggc ccaagacgcc tgcggccctc
     7741 gaggcccgca accggctggc ggccatcggc gagcctctcg aggaacccgc gatcctcgag
     7801 ccgcaggcct caaccaacgc cgtccgtcat caggagacgc agcgctgatg caacctacat
     7861 ctcatcatca tcgatctgtc gccgccctcg atatcggacg tctggccctt ctggccggga
     7921 gcctcctggt ttgcgggtgc ggctacagta acgagtcgct gcatccccag acgatccgta
     7981 ccgtgtacgt ggagatgttc cagtcccggg aattccgccg cggcatcgag ttcgagctga
     8041 ccgaggccct gcgaaaggaa atcaacgtct ccaccccata cacgaacgcc ccacccgaaa
     8101 aagccgatac cattctgtcg ggggaagtcc tggaatggcg ggaatcttcc ctcggctggg
     8161 atccgatcgc catccgaccg cgcgagacgg cagccacatt gatcgtccgc taccgatgga
     8221 aggatgtcag gacgggcaag ctgctgcggg accgtccgcg gtttgtcacc accgtgactt
     8281 atgtgcggcc ggccggcgaa acggcggccg aaggcagact ggacgcggtc agcaagctgg
     8341 cacgaaacat cgtcggcgac atggaaaaca gttggtagtc cgaataacat gaaggcgggg
     8401 gaaggacgcc agttgcggcg cctgcccgtc gacacgccga ccgaggaagc accggatgtc
     8461 tgaaaacccg tccgaccgcg atgatcgcca gggatcaagg cctcccggac cgcccatgcg
     8521 catgtcccgc ggtctgttcg gatgggcggc ctcgatcatc atcttcgtct tcctcgcttt
     8581 ctacctcatg tctaacgccg gccagcgccg tgaattgacc gtggaccagt tctggaaaga
     8641 gctggcggac aacaacatcc aggaactggt catccaggcc aacaacaaca ccatccgagg
     8701 caagctcaag aacacccagg gcaaggacca gccgccggac ttcgaggtca gctgggacaa
     8761 atccgccttc aacgatgcct tctggaccga gttgcgggcg tccggcgtcc gttacaggat
     8821 cgaccagacc ggcggggcgc tcttcaatat cctgatcaac ttgctgccct ggctcctggt
     8881 catcgcgttc gcctggttct tcgtcttccg ccaactccgc ggagtcggcg gcggaccggg
     8941 aggtatgctg ggcaacttcg gccgatcccg acatcgcgtg accaccaagg agcataccaa
     9001 catcaccttc gccgacgtcg cgggcatcga cgaggccaag gaggagtgcg ccgagatcat
     9061 cgagttcctc aagaacccca agaagttcca gcggctcggg gcccgtattc cgcgcggcgt
     9121 gctgctggtc ggcgatccgg gctgcggcaa aacgctgttg gccaaagcca tcgccggcga
     9181 ggcggacgtg ccgttcttca gcatctcggg cagcgatttc gtggagatgt tcgtcggcgt
     9241 cggtgcctcg cgcgtgcgcg atctgttcaa gcaggcaaag gacaactcgc cttgcatcat
     9301 ctttctggat gaaatcgacg ccgtcggccg tcgccgcgga gcaggcttca ccagcggcgg
     9361 acacgacgaa agggaacaga cgctcaacgc catcctcgtc gagatggacg gcttcgacac
     9421 ttccgatcag gtggtggtca tcgccgctac gaaccgggcg gacgtgttgg atccggcact
     9481 ggtaaggccc gggcgtttcg accggcaggt cttcgtgccc ctggccgacg tcaagggccg
     9541 gctggaaatc cttaaggtcc acgccaagaa ggtgaagctc ggcccggacg tggatctgaa
     9601 taagctggcc cgcggcacgc cgatgttcag cggggcggac ctcgcggcga tcatcaatga
     9661 ggcggccttg ctggccacta tggccaacaa ggattacgtc gaaatggagg atctcgagga
     9721 ggctcgtgac aaggtccgat ggggccgggc caagaagagc cgcgtggtgg atgagaagga
     9781 acggatcgcc accgcctacc acgaagccgg ccacgccgtg gcccaatgcc tgttgcccga
     9841 cgccgatcct ctgcacaaag taagtatcat accccgcggc ccctacggcg gtgcctcatt
     9901 ctcgttgccc gaaaaggacc gcacaactta cagcagcaag tggctcaacg cacagttgcg
     9961 cattctctgc gccggacgca tcgccgagga aatcacctgc aacgacgtca acacgggggc
    10021 ctctgctgac attcgacagg ccaccgaaac ggctcgccgc atggttgccg agtggggcat
    10081 gagcgaccgc gtgggcttca tctactacgg cgacgatccc tcccgcaaga cgatgtgggt
    10141 cgacctgccc ggaggcaagg agtattccga ggaaaccgcc cggttgatcg accaggaagt
    10201 tcagcgcgtg ctcagcgagg cttataccga cacccgtcgg ctgctgaccg agaatcgtca
    10261 gaaactggag gcagtagccc aggccctcct gaagtacgag acgctcgatg ccgctgaggt
    10321 tcacgcgttg ctgaggggtg aaagcctcga tcgccccacg gtgtccgatc tgctggacgc
    10381 tgctcacgcg ggaatcggcg tccgcgcttc ggccggtccg ggtacctcga agtcttcaca
    10441 gcctgagttc ggctcggggc cgctgcccca gccgggatga gatgagcgtc atcgatgctt
    10501 tctgaaccct gcggagatcg tgcatggcca acgccgaaat cattcccgtc gggatgaacg
    10561 acctgccgct catcgtcgat ctctacaacg agatctttcg cccgggccgc gagcacgctt
    10621 tcttcgctca tcgtctgggc acacagcgca acccgttgct tctcctcgcc caggtccaga
    10681 agcgcccggt cggctttgcc ctcggttacg agctcaagca gagcacgttt tactgctggt
    10741 acatcggcgt cttgccggaa ttccgacgcg ccggcgtcgc ctcccagatg atggaggcca
    10801 tgacggcatg ggctcgcgac cagggttacc agatcatccg cttcgagtgc tacaaccgac
    10861 atcgcccgat gcttcacgtc gccatccggc agaactacaa cgtcgtcggc attcggtatg
    10921 acgccgacgc cgaggacaat ctgattattc tcgaacaaaa cgttaatgaa gaagctgccg
    10981 attagcatca acggtcgcaa gcgggcaaac ccgcgcgcgg attcacgacc aactgggtct
    11041 gataactcgc cgacggacgc gagacacgcc ctgttgcagt cagggtgaga tcagtgagag
    11101 tcccggccgg cgggccgccc aggcgtttcg cgagtgaatg cggcggccgg cgtgtcagcg
    11161 gagccgcgac cgactcggag ttcgaccgct gcggcccaac gtgagctgca cgcacgaaat
    11221 gcggccgcgg caaacaggag cctttcaatg gctgacgaga atcgaattga acgcatcggc
    11281 atcttgaccg gcggcggaga ctgccccgga ctcaacgcgg tgatccgagc ggtcgccaag
    11341 gcagcgatgc atcaacatgg cttggaggtc tggggaatcg aagacggttt cctcggcctc
    11401 atccgcaacc gtatgcggct cttgggccgc gacgacgtgt ccaacatcct gacgcgaggc
    11461 ggcaccatcc tgggcacctc gaacaccgcc aatccgtcgc gtttcgcggt gggcatcgac
    11521 gaacaaggaa atccggttgt tcaggatgtc acccagcgag tcatcgagca catcgccgaa
    11581 cgacagcttg atgtgattgt ctgcgtcgga ggcgatggaa caatgtccgg tgcggcgagc
    11641 ctcattcagc acggtgtccg atgcgtcggg gtgcccaaaa ccatcgataa tgatctcatg
    11701 cacacggaaa tcaccttcgg cttcctgacc gcggtaaaca ccgccaccga ggctctcgac
    11761 cgcatccaca ccaccgcatc cagccaccat cgcgtaatgc tcgtcgagat catgggacga
    11821 aatgccggct ggctggcatt gaccagcggc ctggccagcg gggcggacgt catcctcatc
    11881 cccgagattc cctacagtct tgaggccatc tgcgactact gtctcaaccg cagcaagcga
    11941 ggcaagtcat tcaccatcat cgcggtgtct gagggagcca gacctattgg aggcgaacag
    12001 acggtcgacc gcgtggtcgc cggtagcccc gacccgatcc gcctcggcgg cgtcagtaac
    12061 gtcctttgca aacagatcga ggccgcgacg cgcctcgagt gccgcgcgac cattctcgga
    12121 cacgtccagc gcggcggcac gccggtgccc gccgaccgtg tcctgggcac gctcttcgga
    12181 cacaaagccc tcgaactggc cctcgccgga aagttcaacg aaatcgtggt cagccagaac
    12241 gggcgaatca ccagcgtgcc catcgagtcc gtggccggcc ggcagcgact ggtgcccctg
    12301 gatcacccgc tgatcgcggc agcccgatcc gtgggaacca gcttcggcga cggcctgtga
    12361 gcagaccccg ccgaccgacg tcgtgcatgc tgcccgcaga cccggggacg atgccgcatc
    12421 ttgtgcatgt ctccgtcgcg ccacgatcat ttctcggcgg ccagccggcg caggatatcc
    12481 cagtaagccc cgcgcatgcc gcggccgatc gcatcggtca gcacccggtt gtcattgtag
    12541 ttgtagttgg tcgcgtctct gatccgcggc ggcgggaaga accgctcgtc gccggcgatg
    12601 atgtcgcaga ccagcgagcc gggatcatcg ctgagacggc gatcaaagta aaaaaccggt
    12661 cgcaagaggc tgtcattgtc ttcgacgcag ccccgcagat tggggttggc cgacagcggc
    12721 ccctgcctgc gaatcgactc ggccagcgga gtacccggat aaagccgcac ccccgtggcc
    12781 gccccgcagc ggtcgggatc gagtcgcctg acgaactcga ttgtctctct caccgacgcc
    12841 tcgtcctcgc ccgggcctcc tagaagcagg tcgaccatca ccgtgatccc tgccgccttg
    12901 caggcctgca ccgcgtcgcg aattgcgtct cgccgatagc tcctgcccag cgccgccagc
    12961 atgcggtcgc aaccgctgtc ggagccgaag ttgacgccca cgcaaccggc ctgcttcatg
    13021 gctagggcga gttcatcgga gaacgggtgc ggactgcaat agacatacca ccggacgcgc
    13081 tgccccagcc cccgccggat catctcgcgg caaaccgcca gggcgtgatc atacggaatg
    13141 ttgaactctc cgtcgcacag gtgcagcacg tcgacgcctt gcttgagcag cgactcaacc
    13201 tcatcagcca cctgggtggg cgggcggcaa cgcacctgtc gccccttggc aaccgggtcg
    13261 gcacagtaga tgcacgacat cggacagcct cgcttggtct cgacgttgcc ctgaccgcct
    13321 tcgcggaagt agcgggcgtt gtcgagcagg ctgcggtcag gcgagacgtc gagaacggcc
    13381 ggcggctgag gcggattgag catgcaccgg ccgtcggctt cgcgccaaac cagaccaggc
    13441 agcgtgtggt aatccttatt gcccgacagt gcacgagcca gccccagaaa accctcctcg
    13501 ccatcgccga tgatgccgaa atccgacttg cagatgctca cgatctcggc gggaaagacg
    13561 ctgaatccgc atccgccgag agcgaccggg gcatcggtta gcgtggggat ctgccgcacc
    13621 agttctgcta gctccggcac gaagaacgcg caactcgggt aatagcagtc atccgtattg
    13681 cggaacgtca ccccgatcag cagcggttca gcatcggcca ggccctgccg caatgcctcc
    13741 gctgggtcat ccgcaaagca cagatcgatc aggtgaacct caaaccctcc gcgacgcaga
    13801 ctccccgcga catagtccag ggccagcggc gcgatcgccg gtttcatccg gttggagttg
    13861 atcagtacaa cgggtcgatt cgccatttcg agcctccgcg acgcactata gcccgtaaac
    13921 gaatcgggtc aacggaggga atcggcttct tcctgtcgac gaccgctctc atgcttcctt
    13981 ccgctccggc agtttccatc cgcattccgg gcacacgccg ctggtattgc cggtcaggtt
    14041 gtagccgcac cggccgcaga agccgggatc gagttcgtac agctttccga aatacttgtc
    14101 ggaacgcagg ctccgcaggc cgaaaacacc gcacgccaat gcgagaacca gaagcggtag
    14161 catgatcata agaccgagcg tgccattgcc gctttggcgg aaactgaaga tcgcctgggc
    14221 gcacacgaac acggtgaacg ccatgttcca gagcaggttg atcttcgcgt acctgcgacg
    14281 ttcgttacct cgggccttgc gggacttgat gatcaacacc acgaacacgc aggcgcacag
    14341 aaccgcgatg acggtaagac ctatcgagat atgcagtgta ctcaatccgt gactccctat
    14401 ggcgagaggc gccattggcg ggcaaagccc gccctagtgc gtctgagagt gtactcgatc
    14461 agaggggtgc aagtcccctc caggcctgcg
//
LOCUS       DAOTPW010000143        14350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4134_length_14350_cov_5.827492, whole genome shotgun sequence.
ACCESSION   DAOTPW010000143 DAOTPW010000000
VERSION     DAOTPW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14350
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4134_length_14350_cov_5.827492"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="P5316_13455"
     CDS             <1..375
                     /locus_tag="P5316_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRR86011.1"
                     /translation="SDHRWSCLMVRWSTKTLGESEKHKAGITGDTVGDPLKDTAGPAI
                     NPLIKVMNMVSLLIIGLILPYDHEIIAKLGEHNVQVAGKSPDALFWAVVVVGVGGLVW
                     ALWQSKRETPQRGSFKAALTGG"
     gene            544..2079
                     /locus_tag="P5316_13460"
     CDS             544..2079
                     /locus_tag="P5316_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRR86012.1"
                     /translation="MRFQSILIPSLDDSAVQVAGEVPAFFRDLNLDQIVEAITAGRRE
                     YDLAPFFYAPLHDLDAIAYRQEVMQDFEDPNLIQVVGSFSRRMHEIRVHLPQPKEHYY
                     RYERERWFLDAVSIYCEAVGELAHRLAEFDLGSRGMRTLREYLTEYVASDSFRKLAAE
                     ATSLRSALSAIKYCLLIHGGRITVLRYEGEGDYSAAVEASFQKFRRGAAKDYRVKDLC
                     SESMNHIQAQVVERLARLHPDTFAALEAYYTEHADYLDETISRFDREVQFYVAYLAHI
                     ARFRSAGLSFCYPRLSRESKEISARRTFDLALAAALLDNEATVVTNDFFLRGPERIFV
                     VSGPNHGGKTTFARTFGQLHYLAALGCPVPGTEARLFLYDRLFTHFEREEDIETLRGK
                     LQDDLFRIRSILDQATPNSIVVINEIFSSTTLKDAVYLGQRIMARLSALDLLGVCVTF
                     LEELASFNEKTVSVVSTVEPDDPAIRTYKLERRPADGLAYALAVAERHHVTYDWLMRR
                     IKA"
     gene            2076..3767
                     /locus_tag="P5316_13465"
     CDS             2076..3767
                     /locus_tag="P5316_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRR86013.1"
                     /translation="MKALLMHRDRDFDLAQGLPWNESVLTQDLELNTLLDAMAGGDRF
                     LFDVARRGLFSGLRSDVDTVLYRQAILKDCLKNPAAVREFYDLTVETIKRKRTGWLGI
                     LSRQPAGILSESIALLEMLIGMLRKLRGIAKTHADRFDSEGFKALFAMIERELSDDYL
                     AGICHHLTELKFRKGLLLSAELGEGNAGINYTLCQAHKKRPSWLRRILGQGSPAYTFR
                     LADRDEAGARILSQMRNCGTNLVANALAQSADHVRSFFEMLRTELAFYVGCLNLHERL
                     ASKGEPVCFPCPVPAGERRLRFSGLYDPCLSLLTVHRVVGNAVEADGKNLVIITGANQ
                     GGKSVFLRSIGLAQMMMQCGMFVAARSFEAELCTGLFTHYKREEDATMKYGKLDEELA
                     RMNDIADHLAPNAMLLCNESFAATNQREGSEIARQIACALLERRIKVFLVTHLYEFAR
                     GLFDKRREDALFLRAERQADGSRTFRLIEGEPLETSYGEDLYREVFPDGTDKEERNEV
                     PSDRHRDQEDRPAPAGPGADLRQPVRTKGMNHDICRDDDLAGNCMRPRAGLDAGG"
     gene            3754..4293
                     /locus_tag="P5316_13470"
     CDS             3754..4293
                     /locus_tag="P5316_13470"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86014.1"
                     /translation="MPEDEPVPRCEVSSLSVPDEKTRVEHAMRRLLRVTFGAFDLGAD
                     KMAGTAGSLEANPMLGCRQIRVFPDHPEQSICQMRALLRMMRRIIPAGHRHNRPVSLC
                     GEMASDPIYTIILLGLGVDELSVNPVMVPAIRALRPDVCAPCAHVSGCSALGLQPGKK
                     GVCLRGSHLFPDLNRSITH"
     gene            4410..4943
                     /locus_tag="P5316_13475"
     CDS             4410..4943
                     /locus_tag="P5316_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86015.1"
                     /translation="MDLRLGPRTLELATELAASVALAVGSMQPLPLLLIDDHRPYPAA
                     ILQVFGLIRHRRRKRGRGRRKHPDLKPPPGLWVGVVRKVRNATGKLLRVRTWALFGRK
                     RDIERRMRRLRLGSRINTAHIERLNGSLRGQQARLARRTRNGSRLAEALQWSLGLWRD
                     LHNRTRPTVRPMDTRPQ"
     gene            complement(5886..6806)
                     /locus_tag="P5316_13480"
     CDS             complement(5886..6806)
                     /locus_tag="P5316_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86016.1"
                     /translation="MRQRNVLTIGMGLLVSVLMADAASAYYSPRLGRFLNRDPIAEPG
                     AVLVRQAARPATSFIPRDPIDGERANGNVYRFVDNNPIIYVDPYGLWKIERNGEPRAV
                     VTSEQGDTIQTLAELVKLEPSEYKLWLKAMNDGIPQSSTAKIGADCKYTVPNTAYVHL
                     GHIKVGWKFWAKGGDAADLWWAVMEWKDIASDFRNQGYFVSDIRGATKAVVLSHLGNE
                     NIAAWAIVAHGAEGIIWAADAAENDDELVGISSYDGTSSQHHKLSTVILYVCEAKKSK
                     NWENMISQFGTIWWTEGKVRMWHDWDDLGN"
     gene            complement(6829..7887)
                     /locus_tag="P5316_13485"
     CDS             complement(6829..7887)
                     /locus_tag="P5316_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRR86017.1"
                     /translation="MSQSIPASHYDLLAMTPEQLAKIDVALVNLLCAKGLPGAENLDI
                     PAILAKLDEWAAKVKFETLRHLYRVTDPRYAEHYNHSEARLRAEFIVQCLQEDCGVRY
                     NPDRIYEPDFRDSRDMFLHGMLPGANGGTCASMPVMYVAIGRRLGYPMKLVATREHLF
                     CRWDDGKERFNIEGATNGGVSYHPDEYYRSWPKPLSEADMATGVFLTSMSPEAELADF
                     LHHRGMCMHQNGRLTEARAAFAEAHRLQPRDTNTFTALRVVCGAVPRPRYAQNPAAEA
                     WQYGPRPPDPDDPTAQIYQQMTGPSVGTYGQPVQPGAPTQHQPGIPNYSGHPGVPGQP
                     QQPGFAGQHQTGYPNASR"
     gene            8412..8930
                     /locus_tag="P5316_13490"
     CDS             8412..8930
                     /locus_tag="P5316_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86018.1"
                     /translation="MQTTGLTFDEYVDLRLRPPAGADRAFHEAAEMERERMFPMTLTA
                     ASSHLRSRGYDCKPAMLEMLIENRVVAPAGPDAWSQGDVDAAAEHFEDCQLFTPYAAM
                     CVALGCRYADFLRPLREAAERESTRYGRRVPDDDQYFVMHRVPPRETSPAVISFTLCD
                     DIRERLERGEEV"
     gene            8931..9521
                     /locus_tag="P5316_13495"
     CDS             8931..9521
                     /locus_tag="P5316_13495"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR86019.1"
                     /translation="MNPSPQELKLTPDLLEYARAVALKEAPKHCGSRISYDDVVQEAI
                     LHLLSRPPKFDPSRGASEKTLIYTIVQRAVIKYAERERKHVSPLRPFDEPADESSGAL
                     AGPTEDQMRTHRRLTGKGPTKHATLQAVVDEMLIYIDNEESRALCRLFIECDGNTSAT
                     ARRMGMTEGAIRYRLKMLAPKLLAAGFNPFSQGGDT"
     gene            9518..11407
                     /locus_tag="P5316_13500"
     CDS             9518..11407
                     /locus_tag="P5316_13500"
                     /inference="COORDINATES: protein motif:HMM:NF021628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HRR86020.1"
                     /translation="MTAIATDNLLLTAPEVAIEAGGEPKRPRISITAYTGGLMRVPGW
                     GDVVIDLAGLDAAGQIPLLADHDARVGGVVGHGEARVTDGRLVVAGVMSGAGEAARQI
                     VEMTSGGFAFQASVGVEPVEHERIKPRERVQVNGRSLSSPRGFALVKQGRLREVSITP
                     LGADAGTSVAIAASGHAKEWLHMNTDVQNADEQEIRANERQRIAEIENLCRKPDGGWG
                     RMQAKVDELKASAIAGELSAQDLSAELLNILRESRPKAPMAYTPQPVGGVATLEAALL
                     KRLGLTALGEKALGAIAMEHGDRLKATHTLDICRAALMLDGADVPHGREEMVRASLSL
                     ASLPQALGNLANKLLLDAYDEAPATWRAFCSVRSVADFKPHTAIRPSFTGQLKHVAPG
                     GEIKHGQVDEWFTQFQVDTFGKMLSIDRRDIINDDLGVFDETARAFGRAAMRKLSDLV
                     YETLLGNANGFFSTANGNYIEGADAALSFDSLALAITAMMLQRDDEGNDLDLRPATLL
                     VPPELQPTAKALLESEFIQQIAEKMPTGNSLRRAVSIEVEPRLSNSAKFGAAASDKHW
                     YLFTSPANTPMVTAFLNGRQQPTVEFFGLDQDVNTLAVSWRVYHDFGAALVDPRAAVR
                     SKGQA"
     gene            11568..11753
                     /locus_tag="P5316_13505"
     CDS             11568..11753
                     /locus_tag="P5316_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86021.1"
                     /translation="MTTVTLDRKDATDRVGDFEMRVVTGERTSESEERWSRRSEMIAT
                     WLLDEWKRQQREIAQRN"
     gene            11756..13372
                     /locus_tag="P5316_13510"
     CDS             11756..13372
                     /locus_tag="P5316_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HRR86022.1"
                     /translation="MLAISQSTAESDLVRHYDDGAGRPNATREKERAFRQGFLEEAQR
                     IVREWIDPDEDTFDPWGEEQAALQLRLRVRERYTVYGAGYGPGCAGRKTYHLGYRPGH
                     GVPSARARGRHHALAYVTQAWDEMDDLDTFERLILPAWLTAVERWVREPIRPSVITAP
                     PRPLEIPGVADYLDDMRQRKAPSTEIMRNESGRLVPLCGSQGGTASPRRLELVRADQI
                     EMRPPDWLLRGMLERDTFALVFGDPGSGKSFLAIDWACRVVTGTPWRSHDVTQGPVVY
                     VAGEGQQGFGRRIRAWSEYNGVSLADAPLYVAPAVAIPEARDLIALVQAIHDVAGKPT
                     LIVLDTLARCFGGGDENSTQDMSQFVSACDVLRRQYGCTILVVHHTGHADKNRARGAI
                     ALKAALDAEYRLVNDDKLLLTATKMKDSETPPPVAMELVPVELPGIVDEYGNPVTSAA
                     IEVLDADTGAIISKAKTIRPRGKWQDVGFGVARRLVAASDDGCVSINAWRDECQAAGM
                     VRQNQHRVLEGLVNRGEIAVSDGVLSIVTT"
     gene            13708..13926
                     /locus_tag="P5316_13515"
     CDS             13708..13926
                     /locus_tag="P5316_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86023.1"
                     /translation="MNGFLTRQTLLEATWRRLGDSRGCYRRRCVECGHILYASRPEAQ
                     YCRPACRQRAYRRRVRFRRESALMFSAN"
     gene            13976..>14350
                     /locus_tag="P5316_13520"
     CDS             13976..>14350
                     /locus_tag="P5316_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRR86024.1"
                     /translation="MIATTDTIPAVAYLRRSTDKQEQSIGDQRLEIARHAEEQGYQII
                     REYVDDAISGTSADERPGFQRMIADAARGDFAAVIVWNSDRFSRGDVTETEHYRYLLR
                     QANVKVLSVTEDYLARDGIDGDV"
BASE COUNT         2786 a         4258 c         4590 g         2716 t
ORIGIN      
        1 agcgatcatc ggtggagctg cttaatggtg cgttggtcaa cgaaaacact cggagaaagc
       61 gagaaacaca aggccggcat caccggcgat accgtcggcg atcctctgaa ggacacggcg
      121 gggccggcga tcaatccgct gatcaaggtc atgaacatgg tcagcctgct gatcatcggg
      181 ctgatcctac cgtacgatca cgagatcatc gccaagcttg gcgaacacaa tgtccaggtg
      241 gccggcaaga gtccggacgc cttgttctgg gcggtggttg tcgtgggcgt tggcggcctc
      301 gtgtgggccc tctggcagtc gaagcgagag acgccccagc ggggttcgtt caaggcagca
      361 ctgacagggg gatagagtgg gcagagacgt caatcgcagt gaacaccgtc cggctcatgt
      421 cagcgtgtcc ggttccgggg cgcatcggga atgtctgaca cgggccctgc tctcggatgc
      481 cgggaagcag cgcggacggc cttcgccgga ctcgatacgt tccgtcattg aggcactcat
      541 cccatgcgtt tccagagtat tctgattcca agcctcgatg attctgctgt gcaggtggcc
      601 ggtgaagtcc ccgccttctt tcgtgacctg aatctcgacc agatcgtgga ggccatcacg
      661 gcgggccggc gggaatacga cctcgcgccg ttcttctatg cgcccctaca tgaccttgat
      721 gcgattgctt atcgtcagga ggtcatgcag gatttcgagg acccgaacct gattcaggtc
      781 gtcgggtcct tttcccggcg gatgcacgaa atacgcgtgc atctgccgca accgaaggag
      841 cactactaca ggtacgaaag ggaacgatgg tttctggacg cggtgagcat ctattgcgag
      901 gccgtcgggg aacttgccca tcgcctcgct gagttcgacc tgggatcgcg aggcatgcgt
      961 acccttcgcg agtatctgac ggagtatgta gcatcggact ccttccgaaa gctcgccgca
     1021 gaggcgacat cgctcaggtc cgccctctcc gccatcaagt actgcctgct catccacggt
     1081 ggccgcatca cggtcctccg gtacgaaggg gaaggcgact atagcgccgc ggttgaggca
     1141 agcttccaga agttccggcg cggcgcggca aaggactatc gggtcaagga cttgtgctcg
     1201 gaaagcatga accacattca ggcgcaggtg gtggagcgac tggcgcgcct tcatcccgac
     1261 acatttgccg ccctggaagc ctattacacg gagcacgcgg attacctgga cgagacgata
     1321 tcgcggttcg accgggaggt tcagttctac gtcgcttatc tggcgcacat cgcaagattc
     1381 cggagcgccg gcctgagttt ctgctacccg cggctttccc gcgagtccaa ggagatcagc
     1441 gctcgccgca cgttcgatct cgcgctggcc gccgcgctcc tggacaacga ggcgaccgtc
     1501 gtgacgaacg acttcttcct gcgcggcccg gagcgcatct tcgtcgtctc cggcccgaac
     1561 cacggcggca agacaacgtt tgcccggacc ttcggccagt tacactattt ggccgctttg
     1621 ggctgtcccg tcccgggcac cgaggcgagg cttttcctgt acgaccgtct cttcacgcac
     1681 ttcgagcgcg aagaggacat cgagacgctg cgcggcaaac tgcaggacga cttgttccgg
     1741 atccgctcga tactggacca agcgacgccg aacagtatcg tcgtcatcaa cgagatcttc
     1801 tcgtcaacga cacttaagga cgcggtctat ctgggccaga ggatcatggc gaggctttcc
     1861 gcgttggacc tgcttggcgt gtgcgtcacc tttctggaag aattggcctc cttcaacgag
     1921 aaaacggtca gcgtcgtcag caccgtcgag ccggatgatc cggcgatccg cacttacaaa
     1981 ttggagcgga ggccggccga cgggctcgcg tatgccctgg cggtcgccga gaggcaccac
     2041 gtgacctacg actggttgat gaggcggatc aaggcatgaa agcactgctg atgcatcggg
     2101 atcgcgattt cgacctggcg cagggacttc cgtggaacga atccgtgctg acgcaggacc
     2161 tggaactgaa taccctcctg gacgccatgg cgggcggcga ccggttcctg ttcgacgtgg
     2221 cgcgcagggg gctcttctcg ggcctgcgga gcgacgtgga taccgtcctg taccggcagg
     2281 cgatcttgaa ggactgcctg aagaaccctg cggcggtcag ggagttctat gatctcacgg
     2341 tggagacgat caagaggaag aggaccggct ggctcggcat cctcagccgc cagccggccg
     2401 gaatcctgag cgagtcgatc gcgctgctgg agatgcttat cggcatgctc aggaagctga
     2461 ggggcatcgc caagacgcac gctgaccgat tcgactcaga gggcttcaag gccctttttg
     2521 cgatgatcga gcgggaactg agcgacgact acctcgccgg catctgtcat cacctgacgg
     2581 agctgaagtt tcgcaagggt ctgttgctga gcgctgagct gggcgaaggc aacgcgggca
     2641 tcaactacac gctatgccaa gcgcacaaga agaggccgag ctggctccgg cgcatcctcg
     2701 gccagggatc accagcgtac acctttcgcc tcgccgatcg cgacgaggcc ggcgccagga
     2761 tcctgtcgca gatgcggaac tgcgggacca acctggtggc gaacgccctc gctcaatccg
     2821 ccgatcacgt ccgcagcttc ttcgagatgc tgcggacgga gttggccttc tacgtcggct
     2881 gcctgaatct gcacgagcga cttgcgtcca agggagaacc ggtgtgcttt ccctgtccgg
     2941 ttcccgcggg tgaacgcagg ctacgtttca gcggtttgta tgacccctgc ctttcgttgc
     3001 tcacggtaca tcgagtggta ggtaatgccg tcgaggccga cgggaagaac ctcgtcatca
     3061 tcaccggtgc aaaccagggt ggaaagtcgg tctttctgcg cagcatcggt ctggcccaga
     3121 tgatgatgca atgcggcatg tttgtcgcgg cccggtcctt cgaggccgag ctctgcacgg
     3181 gcctgttcac gcactacaag cgggaagagg acgcgacgat gaagtacggg aaactcgacg
     3241 aggagttggc gcgaatgaac gatatcgccg accatctcgc gccgaacgcg atgctgctgt
     3301 gcaatgaatc gttcgccgcc acgaaccagc gtgaaggatc agagatcgcc agacagatcg
     3361 cctgtgcgct gctcgaaagg cgcatcaagg tgttcttggt gacgcacctg tacgagtttg
     3421 cgcgcggcct gttcgataag aggcgggaag acgccctgtt cctgcgcgcc gaacggcagg
     3481 cggacggatc gcgtaccttc agactcatcg agggtgaacc gctggagacc agttatggcg
     3541 aagacctgta ccgggaggtc ttcccggacg gcacagacaa agaggaacgg aatgaggttc
     3601 catcagacag acaccgtgat caggaggatc gccctgcgcc ggccgggccg ggcgccgatc
     3661 tgcgacaacc cgttcgcacg aaaggcatga accatgatat ttgcagggac gacgatctcg
     3721 ccgggaattg catgcggccc cgcgctggtc tggatgccgg aggatgagcc ggtcccgcga
     3781 tgcgaggtgt cgtccttatc ggttccggac gagaagacac gggtcgaaca cgccatgcgc
     3841 aggctgctcc gcgtcacctt cggggccttc gatctcggcg cggacaagat ggccggcact
     3901 gccggcagcc tcgaagcgaa tccaatgctt gggtgccgcc aaatccgcgt gtttccggac
     3961 catcccgagc agtccatctg ccagatgagg gcgttattgc ggatgatgcg gagaatcatt
     4021 ccggccggtc atcgccacaa tcggccggtg agcctgtgcg gggagatggc ttccgacccg
     4081 atttacacca tcatcctgct ggggcttgga gtggacgaac tcagcgtgaa tccggtcatg
     4141 gttccggcca tcagagcctt acggccggac gtctgtgcgc cttgcgcgca tgtttctgga
     4201 tgcagtgcgc ttgggttgca gccaggaaaa aagggggtct gtctcagggg gagtcaccta
     4261 ttccctgacc tgaataggtc tatcacccat tagcccccgt tttgtcaagc gcaaagactc
     4321 ccttggcgaa tccgttggca gatggttcgg cagacgtttc cgcgcaaccg tgttgtcctg
     4381 ctcgcactga atcatcatca tgttgttggt tggatctgcg gctggggccg cggactctgg
     4441 agctggccac cgaactggcg gcctcggtgg ccttggccgt tgggagcatg cagcccctgc
     4501 cgctgctgct catcgacgat catcggccct atccggccgc gatcctgcag gtcttcgggc
     4561 tgatccggca tcgccgtcgc aagcgcggcc gagggcgacg caagcatccc gatctgaaac
     4621 ctccgccggg cttgtgggtt ggggtggtgc ggaaggtccg caatgccacg ggcaagctgc
     4681 tgcgggtccg gacgtgggcc ctcttcggac gcaagcggga catcgagcgg cggatgcgcc
     4741 gtctgcgcct cggcagccgg atcaacaccg cgcacatcga gcgactcaat gggtcgctgc
     4801 gcggtcagca agcgcgtctc gcccggcgaa cgcggaacgg ttcgcggttg gccgaagccc
     4861 tgcagtggtc gttggggttg tggcgcgacc tgcacaaccg gacgcgcccc acggttcgcc
     4921 cgatggacac acgcccgcaa tagcgctcgg actggccgat gcgatatgga cggtgcgtca
     4981 ctatgttcgg tgcgtgttgc atgtcagcga cctgcaccgc atgattggtg cggaggaacg
     5041 cctgaaccgg tttacctccg ccctggatgc ccgaaaacgg aagaagcgct tatcaacatg
     5101 agggactgct ttgtattcgg ggcgaggggc gttggcagta agtcacgacc gggagacggg
     5161 ccagttttcg cgtttacctg ggacacgaac ggaattcgtg aaccatgctg aaaccgaagc
     5221 cagcgatcac gttcattcta cccaacctac gcgctgcttg tctttgagca ccccgctggt
     5281 ggaactggag atgaagacca caagcagatg ccataaggcg acgccggaga cgccttctgt
     5341 tttgctctgt catttctttt ctgagggcga gccatcagcg tcccctgaaa atgtatgtct
     5401 cgacgttctg gcttccagct atcatgtcct gagccgattc tcggctgatc caggatcgct
     5461 tgccatctat gacgagatgg cacctgccag gttcagatgt tgttagaatc agaaaccatc
     5521 cgggatggtc cttgctgtct ccttcgttta tgtagaggat tgagtgaagt cgatcaagct
     5581 gttgattatc ctcagctaga aaaagccgac tagatagaga agggggcaga gagtccagaa
     5641 atgcttgtgc gacggagcca ggttgcgtag tggctccccg tgcatgatcc tggaagctga
     5701 cggcgtaggc caacaaggcg gacctgcgcc acgcctctgt ttttctggta cggtattccc
     5761 aaacgagaag aacaagtccg gcaatgccga cgccaaccaa cagccagacc gcaatggacg
     5821 gctgccatcg catcctcttg ttgtcaccta ccacgcaatc ccattccgtc cttcgtcaat
     5881 aagagtcagt tgccgaggtc atcccaatca tgccacatcc gtaccttccc ttctgtccac
     5941 cagatagtgc cgaactggct tatcatgttc tcccaattct tgctcttctt ggcttcacag
     6001 acgtagagaa tcaccgtcga tagcttgtga tgttgcgacg atgtaccatc atagctactg
     6061 atacctacaa gctcatcgtc attctccgct gcatcggcag cccagataat gccttcggct
     6121 ccatgagcga caatcgccca agcggcgatg ttttcgtttc caagatgtga taaaacgacc
     6181 gctttggttg cccctctgat gtcagaaacg aagtagccct ggttccggaa atcactggca
     6241 atatccttcc actccataac cgcccaccag agatcagccg catcgccccc cttggcccag
     6301 aacttccagc caaccttgat gtgtcccagg tgaacgtagg cggtattggg aacagtatac
     6361 ttgcagtcag ctccaatctt ggctgtcgaa gattggggaa tcccatcatt catcgccttc
     6421 agccatagtt tatattcgct tggctcaagc ttcaccaact ccgcaagcgt ctgaatcgta
     6481 tcgccctgct cggaggtaac aaccgctcgg ggctcgccat tacgctcaat cttccacaga
     6541 ccgtatggat cgacataaat gatcggattg ttgtcaacga aacgatagac gttcccattc
     6601 gccctctcgc cgtcaattgg atcacgggga atgaagctcg ttgccggtct cgccgcttgg
     6661 cggaccagca ccgcgccggg ctcggcaatc gggtctcggt tcaggaagcg gcccaggcgt
     6721 gggctatagt aggccgaggc ggcgtcggcc atcaatacgg aaacgagcaa tcccatcccg
     6781 atcgtaagaa cgtttctttg acgcattttc tttacctctc aatgtcgcct accgactggc
     6841 gttaggataa cctgtttgat gctgtcctgc aaaacctggc tgttggggtt ggccaggaac
     6901 gcccggatgc cccgaatagt tcgggattcc gggctgatgt tgcgtgggag ctcccggctg
     6961 taccggctgc ccgtatgtcc cgaccgatgg tcccgtcatt tgttgataga tttgggctgt
     7021 cgggtcgtcg ggatcgggcg gcctcggtcc gtactgccaa gcttccgcag ctgggttctg
     7081 ggcatacctc ggtcgcggga ccgctccgca gaccactcgc agcgccgtga atgtgttggt
     7141 atctcggggt tgcaggcggt gggcttcggc gaaggccgcg cgggcctctg tcaatcggcc
     7201 attctgatgc atgcacatgc cgcgatggtg cagaaagtcg gccagttccg cctctggtga
     7261 catcgacgtg agaaacacgc cggtcgccat atcggcctcg ctcaacggct tcggccagct
     7321 acggtagtac tcgtccggat ggtagctgac gcccccattg gtcgctccct cgatgttgaa
     7381 ccgctccttg ccgtcatccc agcggcagaa taagtgctcc ctggttgcca cgagcttcat
     7441 cgggtaaccg agccgtctgc caatggccac gtacatgact ggcatggaag cgcaggtacc
     7501 gccgttggca cccggcagca tcccgtgaag gaacatatcg cgagaatctc ggaagtcagg
     7561 ttcgtagatg cgatccgggt tgtaccgaac gccgcaatcc tcctggaggc actggacgat
     7621 gaactcagcc cgcaggcgcg cttccgagtg gttatagtgc tcggcgtaac gaggatcggt
     7681 cacgcggtat aggtggcgca gcgtctcgaa cttgaccttg gcggcccatt catcgagctt
     7741 ggccaggata gcggggatgt cgaggttctc ggcgccgggt agacctttag cgcagagcag
     7801 gttcaccagg gcgacatcga tcttggcgag ttgctcgggc gtcatggcca ggaggtcgta
     7861 gtgcgaggcg ggtatggact gggacacttc ctgaactggc ggctggttcg gccgggctcg
     7921 gctaacagct acgaaggctg cggcggtgac gccagcgccg attacgaaag tcgccaggta
     7981 accgagtttc gtccatcggt ggcttgcggg agctggggag gtttggtgcc gcttacgttc
     8041 tgtgttagcg ggcgagccct tccctgactt gtgtcccgcg cgcgacatga gcgatactcc
     8101 caagttgacc cgtattgtca ccgcgcacac ggccgtgtca agcccgaact tggtccgaac
     8161 ccgctgtggt cacccgtggc ccggcggaga tctcgtggct ggcgacaggg gaatcataga
     8221 accgatagcc tccttatgcg acggtaatcg cgcataacat cattctaaac aatgagttat
     8281 gtgctccagg tgacgaccta acaaaaaaaa cttgcccggc tttttacgaa ttgcccctgt
     8341 cgccggaaat aagtggttga cgagcgagtg gtatgctcgc ggtacaggca tcaaccaagg
     8401 aggtcatgac gatgcagacg acgggactga cgttcgacga gtacgtggac ctgcggctcc
     8461 gaccgccggc aggggcggac cgggcctttc acgaggcggc cgagatggag cgggaacgga
     8521 tgttccccat gacgctgaca gcggcttcga gccatctccg atcgcggggt tacgactgca
     8581 agccggcgat gctggagatg ttgattgaga atcgcgtcgt ggcgcccgcc gggccggacg
     8641 cctggtcgca gggggatgtg gatgctgccg ccgaacattt cgaggactgc cagctcttca
     8701 ccccctacgc ggcgatgtgc gtggcgctgg ggtgtcgcta cgccgacttc ctgcggccgc
     8761 tgcgcgaggc agcggagcgc gagtcgacca ggtacggccg gcgcgtgccc gacgacgatc
     8821 agtacttcgt catgcaccgg gtgccgcctc gggagaccag tccggcggtt atttccttca
     8881 cgctgtgcga tgacatccga gagaggctcg aacgcggcga ggaggtgtaa atgaacccct
     8941 caccgcagga actgaagctg acgcccgacc tgctggaata cgccagggcc gtggctctca
     9001 aggaggcccc caagcactgc gggtcaagga tcagctacga cgacgtggtg caggaagcga
     9061 tcctgcacct gctcagcagg ccgccaaagt tcgacccttc acggggcgcg agcgagaaga
     9121 cactcatcta caccatcgta cagagggccg tcatcaagta cgccgagcgc gagaggaagc
     9181 acgtatcgcc gctacggccg tttgatgaac cggcagacga gtcgtcgggc gcccttgccg
     9241 gcccgacgga agatcagatg cgaacccatc ggcgtctgac gggcaagggc ccgaccaagc
     9301 atgcgacgct gcaggcggtc gtggatgaaa tgctcatcta catcgacaac gaggaaagcc
     9361 gggcgctgtg caggttgttc atcgagtgcg acggcaacac cagcgcgaca gcgcggcgca
     9421 tgggtatgac cgagggcgcc attcgttacc gcctgaagat gctcgcgccg aagctgctgg
     9481 cagcgggatt caatccattc tcgcaaggag gcgacacatg acggccattg ctaccgacaa
     9541 tctgttgctg acggcgcccg aggtcgccat cgaggccggt ggcgaaccca agcgcccgcg
     9601 tatcagcatc acggcataca ccggtgggct gatgcgggtg cccggctggg gtgacgtcgt
     9661 gatcgacttg gccggtttgg acgccgccgg tcagatcccg ctgctggccg accacgacgc
     9721 gcgagtgggc ggcgtggtag gacacggtga ggctcgcgtt accgatggcc gattggtcgt
     9781 cgcgggcgtc atgagtggcg cgggcgaagc ggcccggcag atcgtggaga tgacgtccgg
     9841 cgggttcgcg tttcaggctt cggtcggtgt cgagccggtc gaacacgagc gcatcaagcc
     9901 gcgcgaaaga gtacaggtca atgggcggtc gctgtcatcg ccgcgcgggt tcgcgctggt
     9961 caagcagggc cggcttcggg aagtgagtat cacgccgttg ggcgcggacg caggcacgtc
    10021 cgtagccatc gcggcgtcag gtcatgccaa ggagtggttg catatgaaca cggacgttca
    10081 aaacgcagat gagcaagaga ttcgcgccaa cgagcggcag cggatcgccg agatcgagaa
    10141 cctttgccgc aagccggacg gcggctgggg ccggatgcag gcgaaggtgg acgaactgaa
    10201 ggcctcggct atcgcgggcg agctcagcgc gcaggatctg tcggccgagt tgctgaacat
    10261 cctgcgcgag tcacgaccga aggcgccgat ggcctacact ccgcagcccg tcggcggcgt
    10321 ggccacgttg gaagcggcgc tgttgaagcg actgggcctc accgcgctgg gtgagaaggc
    10381 cctcggcgcg atcgcgatgg agcacggcga tcggctcaaa gcgacccata ccctcgacat
    10441 ctgccgggcg gcgttgatgc tggacggtgc ggacgtaccc cacggacgcg aggagatggt
    10501 ccgcgcgtct ctgtccttgg cttcgctgcc gcaggcgctc ggcaatctgg ccaacaagct
    10561 gctgctggac gcttacgacg aggccccggc gacctggcga gcgttctgtt cggtacggtc
    10621 ggtggccgac ttcaagccgc acaccgcgat ccgcccgagc ttcaccggcc agttgaaaca
    10681 cgtcgcgccg ggcggcgaaa tcaagcacgg ccaggtcgat gagtggttca cgcagtttca
    10741 ggtggatacg ttcggcaaga tgctgagcat cgaccggcgc gacatcatca atgacgacct
    10801 gggcgtgttc gacgagacgg cccgggcgtt cggccgtgcc gcgatgcgga agctgtccga
    10861 cctcgtgtac gagacgttgc tgggcaatgc gaacggcttc ttcagtactg ccaacggcaa
    10921 ctacatcgaa ggcgcggacg cggcgctgag tttcgacagc ctcgcgctgg cgatcacggc
    10981 gatgatgctt cagcgcgatg acgagggcaa cgatctcgat ctgcggccgg ctacgttgct
    11041 cgtgccgccg gagcttcagc cgacggccaa ggcgttgctc gaaagcgagt tcatccagca
    11101 gatcgccgag aagatgccga cgggcaactc gttgcgtcgg gcggtgagca tcgaggtcga
    11161 accgcgtctg agcaacagcg cgaagttcgg cgcggcggcc agcgataagc actggtatct
    11221 gttcaccagc ccggctaata ctccgatggt cacagccttc ctcaacgggc gtcagcagcc
    11281 gacggtcgag ttcttcggtc tcgatcagga cgtgaacacc ttggcggtaa gttggcgcgt
    11341 gtaccatgac ttcggtgccg cactggtcga tccgcgggcc gccgtccgat cgaagggcca
    11401 ggcgtaacgt gatcctgccg tggggcgggc gcgcggatcg gcgatcctca tccacgtcga
    11461 tcacgtcgcc cgccccatca ttgacccgac agccatcaaa ccaccgaccc aacgcacgac
    11521 aacggaagac ggcggttacg gaccgcgcca tggcaatgga gtgcctgatg acaacggtga
    11581 cactggacag gaaggacgcg acggatcgcg tgggcgattt cgagatgcgc gtggtgaccg
    11641 gcgagcgcac gtccgaatcc gaggaacgct ggtcgaggcg gtcggagatg atcgcgacgt
    11701 ggctattgga tgaatggaag cgtcaacagc gtgagattgc ccagaggaac tgatcatgct
    11761 ggcgatcagc caatcaactg ccgagtctga tctggtgcgc cactacgacg acggtgcggg
    11821 ccggcccaac gcgacgcgcg agaaagaacg tgcatttcgc cagggattcc tcgaagaggc
    11881 ccagcgtatc gtgcgcgagt ggatcgatcc tgacgaggac acgttcgatc catggggcga
    11941 ggagcaggcc gcccttcagc ttcggcttcg cgttcgtgag cgctacacgg tgtatggggc
    12001 cgggtacgga cccggctgcg cagggcgaaa gacttatcac ctcggatatc gaccgggaca
    12061 cggagtgccc tccgcccgtg cgcgcggtcg acatcatgct ctcgcgtacg tcacgcaggc
    12121 ttgggacgag atggacgacc tcgatacgtt cgagcggctg atccttccgg cctggctcac
    12181 ggcagttgag cgctgggtcc gtgagccgat tcgaccttcg gtcatcacag cgccgccgag
    12241 gccgttggag ataccgggcg tggcggatta cttggatgac atgcgtcaga gaaaggcacc
    12301 atcaacagag atcatgcgta acgaatccgg caggttggtc ccgctgtgcg gttcgcaggg
    12361 cgggacagcg tctccgcgaa gactcgaact cgtccgcgcc gaccagatcg aaatgcgtcc
    12421 gcccgattgg ctgcttcgcg gcatgctcga acgcgacacg ttcgccctgg tcttcggtga
    12481 tcccggcagc ggcaaatcct tcctggcgat cgactgggca tgccgcgtcg tgacggggac
    12541 accctggcgc agccatgacg tcacgcaagg cccggtcgtc tacgtggccg gagagggcca
    12601 acagggattc ggcagacgca tccgggcgtg gagtgagtac aacggcgtaa gcctggctga
    12661 cgcgccgttg tacgtcgccc ctgcagtggc aattcctgaa gcgcgtgatc tgatcgcact
    12721 cgttcaggcc atccacgatg tggcgggcaa accgacgttg atcgtgttgg acaccctggc
    12781 gcggtgcttt ggtggtggcg atgaaaacag cacccaggac atgagccagt tcgtctccgc
    12841 ctgcgacgtt ctgcggaggc aatacggctg cacgatcctg gtggtgcatc acaccggcca
    12901 tgccgataag aaccgcgccc ggggtgccat cgccctcaag gcggcactcg atgccgagta
    12961 ccgtttggtc aacgacgaca aactgttgct gacagcgacg aagatgaaag actcggagac
    13021 accgccgccg gttgccatgg aactggttcc cgttgaactg cctggcattg tggacgaata
    13081 cggcaacccg gtcacgtcgg cagccatcga ggtgctcgat gccgataccg gcgcaatcat
    13141 atcgaaggcc aagacgatca ggccgcgagg aaaatggcag gacgttggct tcggggtggc
    13201 gcgtcggctg gtcgccgcca gtgacgatgg gtgcgtgtcc atcaacgctt ggcgcgatga
    13261 gtgtcaagcc gccgggatgg ttcggcagaa ccagcatcgg gtgcttgagg gcttggtgaa
    13321 ccgtggtgag atcgccgtga gcgatggtgt tttgagcatc gtcacaacct gaacgtcacg
    13381 cgtcacgccc acgttcgccc ccttaggggg gcgaaacgtg acgtgacgtg gccttgagat
    13441 gagatgtgcc aacgtgacga cgaaacgtga cgcattcaac acattgcgaa tacaagactt
    13501 gtgaagatcg tcacatctcg gcatagcgta aaacgtgacg atgtgacgct acgagatgtg
    13561 acggagcgtc acgtttgcca aacgtgacgc ctgcggcatg ggtcctcccg ggcatcgagc
    13621 cttgccgatg gccttgggaa cagcgcccaa tcccatgaga gtttgtttac cagggtgagc
    13681 attctgccgt aacgccggga ggtggctatg aacggattcc tgacgcgaca aacgctcctg
    13741 gaggccacct ggcgccgtct gggcgacagt cgtggctgtt atcgtcgccg atgtgtggaa
    13801 tgtggccaca tcctgtacgc cagtcggccg gaggcgcaat actgccgccc ggcctgtcgc
    13861 cagcgggcct accggcggcg ggtgcggttt cgccgggaat ccgccttgat gttctcggcg
    13921 aattgagccc tcatggtggc ggtatccgtg aaccgccgac acgaaaggaa acgccatgat
    13981 cgctaccacc gacaccattc ccgccgtcgc gtacctgcgc cgctcgacgg acaagcagga
    14041 gcagagcatt ggtgaccagc ggctcgagat cgcccggcat gccgaggaac agggctatca
    14101 gatcatccgc gagtacgtgg atgatgccat cagcggcacc agcgccgacg agcgcccggg
    14161 ctttcagcgc atgatcgccg acgccgcccg cggcgacttc gcggccgtca tcgtctggaa
    14221 ctccgatcgg ttcagtcgtg gcgacgtgac cgagacggag cactatcgct acctgctgcg
    14281 gcaagcgaac gtaaaggtgc tgtcggtcac cgaagactac ctcgcccgcg atggtatcga
    14341 tggcgacgtg
//
LOCUS       DAOTPW010000144        14326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4145_length_14326_cov_5.756359, whole genome shotgun sequence.
ACCESSION   DAOTPW010000144 DAOTPW010000000
VERSION     DAOTPW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14326
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4145_length_14326_cov_5.756359"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..527
                     /locus_tag="P5316_13525"
     CDS             282..527
                     /locus_tag="P5316_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86025.1"
                     /translation="MRDLLRRQPFVPVRIHLSDGTTYEVRHPEMAIIGRSTVTIGVEE
                     QEGSGIADRMMYCSLLHIVRIEDANGRDLSRKGENPP"
     gene            complement(546..3383)
                     /gene="uvrA"
                     /locus_tag="P5316_13530"
     CDS             complement(546..3383)
                     /gene="uvrA"
                     /locus_tag="P5316_13530"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRR86026.1"
                     /translation="MTLIAGNTDRFIRIQGARQHNLRNISLNIPRDRFVVITGLSGSG
                     KSSLAFDTIHAEGQRKYIETLSAYARQFLQQMTKPDVDHIEGLPPTIAIEQRTGGMNP
                     RSTVATTTEIYDYLRLLMARVGEPHCWVCGQRIDRYSPQEIVDTVMALPPNTRIMVLA
                     PVVRGQRGTHQEVLQQIRREGFVRIRVDGRVYDVKEPPSLAKTRKHTIEVVIDRLTVK
                     PGTNIRLAESVSLALNLGGETVIIAFDRGQGTWQERMYSSRFACPKHPQASVPDLSPG
                     LFSFNSPHGACPTCDGLGHVPEFDEDLVVADASLSLAQGAIDAWQHSGKQLNIFYRKM
                     LNEFCESFKVPPDVPYKNLPAEIRRILMYGTNKETAAKYGARFEGVLPNLKRRWETTD
                     NESVKQRLHEFLSERPCEACGGARLRPEALAVKVGGKNLHDITRMTIEQSHAFFGGLK
                     LTGERTIIASQILREVIQRLRFMIDVGVGYLGLDRASSTLSGGEAQRIRLATQVGSGM
                     VGVCYVLDEPTIGLHPRDNGKLIGTLRRMTDAGNTVLVVEHDEETIRAADYLIDMGPG
                     AGSHGGQVVAAGPINEVLRHPESITAKYLRGEVTIPLPEKRRKVVLAQSIQIKGAAEN
                     NLKHIDVTIPVGCFVCITGVSGSGKSTLINEVFLKGLRRLLGQSRAPAGKHEQIIGAS
                     RVDKVVQIDQSPIGRTPRSNPCTYTGAFDLIRALFARTREAKIRGYRPSRFSFNVKGG
                     RCEACQGQGTKRIEMHFLPDVFVDCDLCKGRRYNRETLEVRYRGKSIADVLDMRVEES
                     IPFFANFERIKHLLLALDDVGLSYMTLGQSSTTLSGGEAQRVKLAAELGKPATDHTLY
                     VLDEPTTGLHFADVHNLLRVLNRLVDKGNSLVVIEHNLEVIKCADWIIDLGPEGGDAG
                     GQVVAAGRPEEIARDERSHTGRFLRSRLATT"
     gene            4009..4854
                     /locus_tag="P5316_13535"
     CDS             4009..4854
                     /locus_tag="P5316_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR86027.1"
                     /translation="MRLRAVRAFSLVEMIAVISIIAVLLSLAVVGVMGVLNSSKAKGT
                     LATMIALKLAIEEFADDRSMASGRGDPRSTTGPDPRKEWRLPAPDNTLGYKAYFGSLP
                     PTPIAQIDLDNPTAPLLNEDIPDQAQQASRQFSNMVRAYLQGANAGPIGREGLLPNGI
                     DVKWRSPKQDPTGEDYASIECLVLFLSQMSTRSRAMMNKLPEACKSDNLDLDRAVLAG
                     TGNYAALYEITDAWDKPLRWAVRRQTSDLVRWELRSAGKDGKFDVGGAFAPIGRTDDV
                     VLQGP"
     gene            4872..5834
                     /locus_tag="P5316_13540"
     CDS             4872..5834
                     /locus_tag="P5316_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR86028.1"
                     /translation="MANGKPTQAAAPKLLALGYRLSVGASSRRSVALNPGPRLTRSGD
                     AKAGSLNPFLASAFTLTELLVVLGILAIVAALAVPALGPMFTSNQTASAISTLSSMLV
                     NAQTIARAQDTAVALRIERAFKTNDSKLMVNIQGLTPYETGIKRADMLKRFIPPPVWL
                     NHQQVRLLKLTGTAFEAPSGGFEPVALPEHVWLAPGEALTNAVAFPSLLENNLKYEPA
                     EGVTYNRFENFMVVFNRFGELVRYPGENCYYLDRTQKFTENHDAYPSVEYPLGQDSSL
                     SLIAYDRRRWENVNDDLARRDLLARTGVPIYINRFSGAILEEKR"
     gene            5831..7366
                     /locus_tag="P5316_13545"
     CDS             5831..7366
                     /locus_tag="P5316_13545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR86029.1"
                     /translation="MKRAENRRQKSEDRRQNAGTPPRFQSFARCGGSPVSCLLFPGFS
                     LLELMIAIVILGIGMVMVATIFPIGIDMAAQTVQMNIAQSVADAALATLKLKVPSISD
                     LTGDSVASYLVFAPDVTATDLDTVIDNSANNNDVLALALSGKKSGPPPPPLTDWMPVD
                     SPWVVEYGPPGLNIASYLTGIFPAGGPPKVRLFTETTGWTGEKGASGPGGAWDYAYVI
                     PSQNLLLNYLTWLSQGKDPRSSMIPPTLGQVPDVYPVNLVDQVYPPVPLIAFDPKKNE
                     YKKRLPADFIPDVAGRRYSWIGIHHVTDPTSGRARFVVTILVTHRSSLTNRYARQAEP
                     SQDKVAGYNPRFIQPDDRRELRLPKPDMDSRTDMLFPHPWLVRFDQINLGTGEVRCTQ
                     AVADLLPPGSYFVAARSVYAFNSSNEQIFPAALAAGTAYEVSARQGQRGNPDDIWTLQ
                     IRRQPPVAGAASEWIPENPSSPRELEIPYAWVVSPAVERVGTGYSFQAKSPVAGVFVR
                     MVE"
     gene            7369..8979
                     /locus_tag="P5316_13550"
     CDS             7369..8979
                     /locus_tag="P5316_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR86030.1"
                     /translation="MRTGEPRIENGEWRKGTHASDGVPNSSFFILHSSFRRAFTLTEL
                     LVSLVILVGMMTLIATIFATAGKSSGAAQAQARMHRQLLQAADAILVDLTNTLPGKDA
                     AEIPKGVLAIAGVTIVARDTVRSAELPHRADVLMLLTQQQFEPFIYQHPPAGQTQFEQ
                     YKQVVYGHADFGELSAAGEWLGVPKFVEKALAKQWYASEWHLARRVVGFRKPPSVVDD
                     STNTRACQWPLVSPAFLGSDATRAVTDVWYDVNNTITFPGILGWMNPGFYAYDRSGSS
                     SGGFGLYECYIERKADSSSGSGGGFFRVDRRQQGEFLLEKDPSDSRVYWWKRPSATGL
                     WKRTDARRTPPDISYMPDQPKPNPVVPSDYVRRGSSDSVHFWPDWFYAPDRVKNPLTD
                     SFRTRLDPTPPPGMPQRTAAYFLPSCSEFKVEFTYDDPSEIAVDPAKGQPILADTNGD
                     GHVGEPYDAGALHDAPAASPINWETVPPGHIFVWTGLPTDPNVYDDLRNRTFPYRWPR
                     ALRITIRAYAPGGALDYPITHTLIHVWK"
     gene            complement(9145..10344)
                     /locus_tag="P5316_13555"
     CDS             complement(9145..10344)
                     /locus_tag="P5316_13555"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HRR86031.1"
                     /translation="MKYAIILPDGAADEPIPQLDGKTALEAARIPNMNWIAATGRSGL
                     VRTIPEDFTPGSDVATLSVIGYDVRAHYTGRAPLEAVANNISLGPDDLVFRCNLVTII
                     DGRMEDFSAGHISQAEATQVINDLQSALGNDRISFHVGVGYRHLMVVKGGISMKVNCQ
                     PPHDIPGEEVAGYLPTGKGSDELRELIAKSQEILANHDVNIVRRDLGENPATSIWLWG
                     QGGKPRLPSFQEKYGLRASAITAVDLIRGIALSIGWKLIQVPGATGYIDTNYRGKGEA
                     AAAALDEMDMIVVHIEAPDEAGHNGDAAAKVKAIEEIDEHIVGPLLDKLKSFDKWRIL
                     IAPDHPTPVIKKTHSAVPPPFCMVGTGVAPDRSERFTESQAEESGLRIDPGHELMEYF
                     LKSGKLA"
     gene            complement(10341..11666)
                     /locus_tag="P5316_13560"
     CDS             complement(10341..11666)
                     /locus_tag="P5316_13560"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRR86032.1"
                     /translation="MSDVFRIGLIGCGTVGQGVVRIIQAEAAEIERKTGVRLELARVV
                     DKDPAQAAKVNVPVGLVSTSASDVFNDKTINVVVELVGGTTVAKDFVLAALNAGKSVV
                     TANKALLAKHGREIYATARRNNCCVAFEASVGGGIPLILPLRTGLIANRINAIYGILN
                     GTCNYILSEMSRNGKPYAMALAEAQAAGYAELDPTLDVNGTDTAHKLAVLASLAFCCD
                     VDFGALHIEGIDQLDAIDLAAGRELGYVCKLLGIAQRTDDGISLRVHPSFVSKKHPLA
                     STSGSFNAVSVYGNWVGHALFYGRGAGSKPTASAVVADIVDIAIGNAGRTFERFRVLS
                     DVTGPPKYVPMEQLRSRYYIRLKVVDRPGVMAAFTRVFGDRQISLKAVTQHETPQVPG
                     DRTATVVVLTDVAQEGHVQAALKEIGSLDIVRESPVMIRVVEEHEEYPT"
     gene            12042..13211
                     /locus_tag="P5316_13565"
     CDS             12042..13211
                     /locus_tag="P5316_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR86033.1"
                     /translation="MIKRDLEPRLKKAAKSFPAVTLTGPRQSGKSTLCQAVFADHPVA
                     NLESPDVRAFASEDPRAFLAQFPKGAIIDEVQRCPQILSYLQGMIDAERKPGRWILTG
                     SQNLALLESVSQSLAGRTAVMQLLPLVHGEVRRFKRHPRSLDEVIFSGSYPGVFDRKL
                     DPKDWYASYVATYVERDVRTVSNVGDLTSFQRFLGICAGRTAQMLNMSSLASDCGIAQ
                     PTVKAWLSVLETCFIAFRLPAFSGNIAKRLVKMPKLHFLDSGLVCWLLGIRSADELRT
                     HPLRGAVFESWVVSEILKHRVNQGEHGHLFYYRDQHGIEADLIVEQGRKLTIVEAKAG
                     QTVTGDMLKSARRVQEILKPLRQTEAIVIHGGSAAQSRDEVKLVPWSTIGKLEWV"
     gene            complement(13526..14263)
                     /locus_tag="P5316_13570"
     CDS             complement(13526..14263)
                     /locus_tag="P5316_13570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRR86034.1"
                     /translation="MSQPIDILFDDEHLVVVNKPAGMLTVPGRQGGVSLREILMRDRM
                     KDTTLLFVHRLDRHTSGVLLMAKTQQAQSFLSVQFQKREVEKDYLALVSGSPEDDSGV
                     IHAPLAPHPRLTGVMVINQRKGKPSVTRWRVEERLGPVTLLRCRPLTGRQHQIRVHLK
                     ALGLPLLVDPVYADSSAFFLSQLKPNYRPSAKHEERPLIDRLSLHAQSLTFVHPGTQT
                     RMRVDAPLPKDFRAVLTQLRKLRDAQS"
BASE COUNT         2581 a         4323 c         4568 g         2854 t
ORIGIN      
        1 tgacgatggg gatcgtcaac ggaggggtcc cagataggaa tctgggacca gcgaatgtga
       61 gtctgggacc ggcgaatggg aatctggcac cggcgaatgg gaatctggca ccggcgaatg
      121 ggaatctagc accagcgaat gggaatctgg caccagcggg tgggaatctg ggagcagcag
      181 gtgggaatct gggaccagcg gggaggtgtc ccatctaagg gctattcgtg cttgccgagc
      241 ggctccaggt tcgctaaact gtctatatgc ggcctgaaga cttgcgagat ctattgcggc
      301 gacagccgtt cgtgccggtc aggatccatc tttccgacgg cacaacgtat gaggtgcgcc
      361 acccggaaat ggccatcatc ggccggtcga ccgtgaccat cggtgtcgag gaacaagaag
      421 gcagcggaat cgccgaccgc atgatgtatt gctcgctcct gcatatcgtt cggattgagg
      481 acgcgaatgg tcgagatctc tctcgtaagg gcgaaaaccc gccctgagcc tttcctcaac
      541 taatctcaag tcgtcgccag gcgtgatcgc agaaaccgcc cggtgtgcga gcgttcatca
      601 cgggcgattt cctccggccg tccggcggcg accacctgtc cgccggcgtc gccgccctcg
      661 gggcccaggt caatgatcca gtcggcgcac ttgatgactt cgagattgtg ttcgatgacc
      721 acgaggctgt tgcccttgtc caccagccgg ttcagcacgc gtagcaggtt gtgcacgtcg
      781 gcgaagtgca ggccggtggt cggctcgtcg agcacgtaca gcgtatgatc ggtggccggc
      841 ttgcctaact cggccgcgag tttgacgcgc tgggcctcgc cgcccgacag cgtggtcgat
      901 gactgcccga gcgtcatgta cgacagcccg acgtcatcca aagccaacag cagatgtttg
      961 atccgctcga aattcgcgaa aaacgggatg ctctcctcaa cccgcatatc gagcacgtcg
     1021 gcgatcgatt tgcctcgata ccgcacctcg agcgtttcgc ggttgtatcg ccggcccttg
     1081 cagaggtcgc aatcgacgaa cacgtcgggc aggaagtgca tctcgatccg cttggtgccc
     1141 tggccttggc aggcctcgca gcgtccgcct ttcacgttga agctgaagcg gctgggccgg
     1201 tacccgcgaa tcttggcctc gcgcgtgcgg gcgaataggg cccggatcag gtcaaacgct
     1261 ccggtgtagg tgcacgggtt gctccgtggc gtgcggccga tcggcgactg atcgatctga
     1321 accaccttat ccacccgcga ggccccgatg atctgctcat gcttgccggc cggggccctc
     1381 gattgaccga gcagccgtcg caggcctttg aggaacacct cgttgatcag cgtgctcttg
     1441 ccgctgcctg acacaccggt gatgcacacg aagcagccta ccggaatggt gacatcgata
     1501 tgtttgaggt tgttctcggc cgcgcccttg atttggatcg actgtgcgag cacgaccttc
     1561 cggcgcttct cgggcagggg gatggtcacc tcgcctcgca ggtacttggc ggttatcgac
     1621 tccgggtgtc gcaggacttc gttgatcggc ccggcggcga cgacctggcc gccatgcgaa
     1681 ccggcaccgg gccccatgtc gatgaggtag tcggccgccc ggatggtctc ttcgtcgtgc
     1741 tcgaccacca ggacggtgtt tcccgcgtcg gtcatgcgcc gcagggtgcc gatcagcttg
     1801 ccattgtcgc gggggtgcag gccgatggtc ggctcatcaa gcacgtagca cacgccgacc
     1861 atgccgctgc cgacctgggt ggccagccga atccgctggg cctcgcctcc cgatagggtc
     1921 gagctggccc ggtcaagccc gaggtacccg acgccgacgt cgatcatgaa ccgcagccgc
     1981 tggatgacct cgcggagaat ctgggacgcg atgattgtcc gctcgccggt cagcttgagc
     2041 ccgccgaaga aggcgtggct ttgctcgatg gtcatccggg tgatgtcgtg caggttcttg
     2101 cctccgacct tgaccgccag ggcctcgggc cggagcctgg ctccaccgca ggcttcgcac
     2161 ggccgttcgc tgagaaactc gtgcagccgc tgcttgacgc tctcgttgtc cgtcgtttcc
     2221 catcggcgct tgaggttggg cagcacgccc tcgaacctgg caccatattt cgcggccgtc
     2281 tccttgttgg tgccgtacat gaggatccga cgaatctcgg ccggcaggtt cttatacggt
     2341 acatccggcg gcaccttgaa gctctcgcag aactcgttga gcattttgcg atagaagatg
     2401 ttgagctgtt tgccgctgtg ttgccatgcg tcgattgccc cctgggcgag ggacagcgac
     2461 gcatcggcca ccacaaggtc ttcgtcgaac tcaggcacgt gccccaggcc gtcgcacgtc
     2521 gggcaagccc cgtgcggcga gttgaagctg aacaggccgg gcgaaagatc gggcacactc
     2581 gcctgcgggt gtttggggca ggcgaaccgc gagctgtaca tccgctcctg ccacgtgccc
     2641 tgtccgcggt cgaaagcgat gatcaccgtt tccccgccga ggttcagcgc cagtgacacg
     2701 ctttcggcca accggatgtt cgtcccgggc ttgaccgtca ggcgatcgat gaccacctcg
     2761 atggtgtgct tgcgcgtctt ggcgagcgag ggcggctctt tcacgtcgta gacccgcccg
     2821 tcgacccgaa tgcgcacgaa cccttcgcgg cgaatctgct gcaagacctc ctggtgggtt
     2881 ccccgctgcc cgcgcaccac gggggccagc accatgatcc gcgtgttcgg ggggagggcc
     2941 atgaccgtgt cgacgatttc ctgcggcgaa tagcggtcga tacgttgtcc gcacacccag
     3001 cagtgcggtt cgcccacgcg ggccatcagg agccgcaggt agtcgtaaat ctcggttgtg
     3061 gtcgcgaccg tcgagcgcgg gttcattccg ccggttcgct gctcgatggc gatggtgggg
     3121 ggcaggcctt cgatgtgatc gacgtccggt ttggtcatct gttgaaggaa ctgcctggcg
     3181 taggccgaga gcgtctcgat gtacttgcgc tgcccctcgg cgtggatggt atcgaaggcc
     3241 agggagcttt tgcccgagcc gctcagtccg gtgatgacca cgaaccgatc gcggggtatg
     3301 ttcagcgaaa tgttgcgcag gttgtgctgc cgggcaccct ggattcggat gaaccggtca
     3361 gtgttgccgg ctatcagtgt catctcgttc gacactccat gttgggcaaa ccccaatgcc
     3421 caagctccaa tggccaatca atgcccaagt ccgaatgacg aaatccgagc gtccgcgcat
     3481 tgacgaatgc ccaattccag aggcgaggga gcatcgctcc ttgggaattg gtcattgagg
     3541 tttggggttt gactggtcat tgggatttcg tcattggggc tttgttatgc tgctcatcct
     3601 gttcttcctg aggaaacaga caatgcgcca atctgccagc ctatgcgatg gtcgggggaa
     3661 tgcaaggaag gagaaagcga gcggtcagtt gtcagcgatc agtcttcagt cggacaactg
     3721 acaccacccc gagtcccctc ctttgtcagc acggccgatt gcgaataaga tcggacagag
     3781 gccgaccgag cctgtcgacg ttccgtaacc ttttgtcaag gctggagatt ggcctgacac
     3841 gtggggtgcc gaatgataca cggtagagaa gcccagttgc atgcgggtga atggttgccg
     3901 cgtgtgcgct taaagtctgg cctgacaaga ctttatgttc gccttccggc aggggagtcg
     3961 ttggctgaag cagcgggggt gaagggcggg ttggaacgga tgaaagccat gcgattgaga
     4021 gccgttcgcg ccttttcgct tgtcgagatg atcgcggtga tttcgatcat cgccgtgctc
     4081 ctgtccttgg ccgtggtcgg cgtcatgggc gtgctgaaca gttccaaagc caaaggcacg
     4141 ctggcgacga tgatcgccct caagctggcc atcgaggaat tcgcagacga ccggtcgatg
     4201 gccagcggtc gcggcgaccc gcgaagcacc accggcccgg accccaggaa ggagtggagg
     4261 ctgcctgctc ccgacaacac cttgggctac aaggcctact tcggttccct gcccccgacg
     4321 cccattgcgc aaatcgacct tgacaacccg accgcaccgc tgctgaacga ggacatcccc
     4381 gaccaggcac agcaggcttc caggcaattc agcaacatgg ttcgcgctta cctgcagggg
     4441 gccaacgccg gtcccattgg tcgagagggt ttgctcccaa acggtatcga tgtgaaatgg
     4501 aggagcccca agcaggaccc caccggcgag gactacgcca gcatcgaatg tctggtcttg
     4561 ttcctgtctc agatgagcac ccgatccagg gccatgatga acaagctgcc ggaagcctgc
     4621 aagagcgata atctggatct cgatcgggcg gtgctggcag gtacgggcaa ctacgctgcc
     4681 ttatatgaga ttacggacgc ctgggacaag ccgctgcggt gggcggtcag gcggcagaca
     4741 agcgatctgg ttcgatggga gcttcgctcg gccggcaagg atgggaaatt tgatgtgggc
     4801 ggcgcgttcg ccccgatcgg ccggaccgac gacgtggtat tgcaggggcc atgaacaacg
     4861 gggagcagag aatggcgaat ggaaagccca cgcaggcagc cgcaccaaag ctgttagctc
     4921 tcggctatcg gctatcggtc ggagcaagca gtcgtcgcag cgtggctctg aaccctgggc
     4981 cccgcttaac acggtccggg gacgcgaagg cgggatccct gaaccctttc ctcgcctcgg
     5041 cttttactct taccgaactc ctggtcgtat tgggcattct ggcgattgtg gcggccttgg
     5101 ccgtgccggc cctgggtccg atgtttacct cgaatcagac cgcctcggcc atcagcactc
     5161 tcagcagcat gctggttaac gcccagacaa tcgctcgggc gcaggacacg gcggttgccc
     5221 tgcggatcga acgggcattc aagaccaacg acagtaagct catggtcaac atccaaggat
     5281 tgacacccta cgagacggga atcaagcgtg ccgacatgtt gaagcgcttc atcccgccgc
     5341 cggtctggct gaatcaccag caggtcaggt tgctgaagct taccggaacg gcgttcgagg
     5401 ctccttccgg gggcttcgag cccgttgcgc tgcccgagca cgtctggctc gcccccggcg
     5461 aggcgctgac caacgccgtg gcgtttccaa gcctgctgga gaacaacctg aaatacgaac
     5521 ctgccgaggg cgttacatac aaccgattcg agaacttcat ggtggtcttc aacaggttcg
     5581 gcgagctggt tcgttacccc ggcgaaaact gttactacct cgaccgcacg cagaagttca
     5641 ctgagaatca tgatgcttat ccctccgtgg agtacccgct gggtcaggat tcgtcgctct
     5701 cgctgatcgc ctatgatcgc aggaggtggg agaacgtcaa cgacgacctt gcgcggcgcg
     5761 atctgctggc tcgcacgggt gtgccgatct acatcaatcg tttcagcggc gcgattctgg
     5821 aggagaagag atgaagagag cggagaacag aagacagaag tcagaagaca ggaggcagaa
     5881 tgccggaacg ccgccgcgtt ttcagtcatt cgcccgctgc ggcggttctc ctgtctcctg
     5941 tctcctgttt cccggcttct cgctgttgga gctcatgatc gccatcgtca ttctgggcat
     6001 cggcatggtc atggtcgcca ccattttccc catcggcatc gacatggccg cccagaccgt
     6061 ccagatgaac attgcccagt cggtcgccga cgcggcgttg gccacgttga agctgaaagt
     6121 gcccagcatc tcggacctga ccggtgattc tgtggcatcc tacctggtct ttgcgccgga
     6181 tgttacggca accgacttgg atacggtcat agacaattcc gccaacaaca acgacgtgct
     6241 cgcgttggct ctgagcggga agaaatcagg gccgccgccg ccgccgctga cggactggat
     6301 gccagtggac tcgccctggg ttgtcgaata cggcccacca ggcttgaaca tagcgagtta
     6361 cctgaccggt atctttccgg caggcggtcc acccaaagtc cgtcttttca ccgagaccac
     6421 cggctggacg ggcgagaagg gggcaagcgg gccgggtggg gcgtgggact acgcctacgt
     6481 gatcccgtct cagaatctgc tgctcaacta tcttacctgg ctatcacaag ggaaggatcc
     6541 gcgtagctcg atgatcccgc ccacgctcgg gcaggttccc gatgtctatc cggtgaacct
     6601 tgtcgatcaa gtctatcctc ccgtgccgct gattgcgttc gaccccaaga agaacgaata
     6661 taagaagcgg cttcccgctg atttcatccc cgacgtggcc gggcggcgat acagttggat
     6721 cggcattcac cacgttaccg atcccacatc cggcagggcg aggttcgtgg tcacgattct
     6781 cgtgacgcac cggagcagtc tgacaaaccg ctacgctcgg caggccgagc cgtcccagga
     6841 caaggtcgcg gggtataacc ctcgcttcat ccagccggat gatcgcaggg agctgagact
     6901 acccaaaccg gatatggatt cgcgcacgga tatgcttttc cctcatcctt ggctggttcg
     6961 tttcgatcag atcaacctcg gtaccggtga agtccgctgc acgcaggcgg tggccgacct
     7021 gcttccaccc ggttcgtact ttgttgcggc tcgatccgtt tatgccttca atagcagcaa
     7081 cgagcagatc ttcccggcgg cgcttgctgc cgggaccgct tacgaggtgt cggcccgtca
     7141 ggggcagagg gggaaccccg acgacatctg gaccttgcag atcaggcgtc agccgccggt
     7201 tgccggcgcg gcatcagagt ggattccgga gaacccgagc agtcctcggg aattggaaat
     7261 cccctatgcg tgggtggttt cgccggccgt cgagcgggtg gggaccggtt acagcttcca
     7321 ggccaagtcg ccggtggccg gtgtgttcgt gaggatggtt gagtaactat gcgaactgga
     7381 gaaccgagaa tcgagaatgg cgagtggaga aagggaacgc acgcctcgga cggggttccc
     7441 aattcttcat tcttcattct ccattcttca tttcgacggg ccttcaccct gaccgagctt
     7501 ctcgtctcgc tggtcattct cgtgggcatg atgacgctga tcgcgacgat ttttgccacg
     7561 gccggcaagt ccagcggcgc ggcccaggct caagcccgca tgcaccgcca gcttctccag
     7621 gccgccgacg cgatactcgt tgacctgacc aacacgctgc cgggcaagga cgctgccgag
     7681 attcccaagg gcgtcttggc catcgcgggt gtcaccattg ttgcccgcga caccgttcga
     7741 tccgctgagc tgcctcaccg tgccgacgtg ctgatgctcc tgacgcagca gcagtttgag
     7801 cccttcatct accagcatcc ccctgccggc cagacgcagt tcgaacagta caaacaggtc
     7861 gtgtacggtc acgccgattt cggcgagttg agtgcggccg gcgagtggct cggcgtcccc
     7921 aaattcgtgg aaaaagcgct tgccaagcag tggtacgctt ccgagtggca cctcgcccgc
     7981 cgggtggtgg gtttccgcaa accgcccagc gtggtcgacg actcgacgaa cacgcgtgcc
     8041 tgccagtggc cgctggtgtc acccgcgttc cttggtagtg acgcgaccag agccgtcacc
     8101 gatgtctggt acgacgtcaa taataccatc accttccccg gcatcctcgg ttggatgaac
     8161 ccggggttct atgcatacga tcgcagcggc tcgtcatccg gcggattcgg tctttacgag
     8221 tgctatatcg agaggaaggc cgacagttct tccggatccg gaggcggttt cttccgagtt
     8281 gacaggcggc agcagggtga gtttctgttg gaaaaagacc cttcagattc gcgcgtctat
     8341 tggtggaagc gccccagcgc cacaggcttg tggaagcgaa cagatgcgcg ccgcacaccg
     8401 ccggacatct cgtacatgcc ggaccagccg aagcctaatc cggttgtccc gtccgattat
     8461 gtcaggaggg gatcgtcaga ttccgtgcat ttctggcccg actggttcta cgctccggat
     8521 cgcgttaaga acccgctgac agattctttt cgcacgcgac tggatcctac gccgccgccg
     8581 ggcatgccgc agcggaccgc ggcttatttc ctgccttcgt gctcggagtt caaggtcgag
     8641 ttcacctatg acgacccgag cgagatcgcc gtcgatccgg ccaaaggtca gccgatcctt
     8701 gcggacacca acggcgatgg gcatgtgggg gagccgtacg acgcgggggc cctgcatgac
     8761 gcgccggccg cctcgccgat taactgggag accgttccgc cgggtcacat ctttgtctgg
     8821 acgggcttgc cgaccgatcc caatgtctat gatgatcttc gcaaccgcac cttcccctat
     8881 cgctggcctc gggccttgcg gatcaccatc cgggcctacg cccccggcgg agctttggat
     8941 tacccaatca cgcataccct gatacacgtg tggaagtgaa gcggtgagca ttcatcgcgc
     9001 agccgttact gcgggtgtgg cctgcggcag gctctcagct tgcttggtcg aatccccaac
     9061 gagaccgtgc ggcgggttgt tctatgaagc atgaacgtca gacgatgcag agcgcggccg
     9121 tccgcggcgt tcgttgacgg cggtttatgc aagcttgccg gacttgagga agtactccat
     9181 aagctcgtgg ccggggtcga ttcggagccc tgattcctcg gcctgggatt cggtgaagcg
     9241 ttcggagcga tcgggggcga cgccggtgcc gaccatgcag aacggcggcg gaaccgcgga
     9301 gtgggttttc ttgattaccg gggtgggatg atctggggcg atcaggatgc gccacttgtc
     9361 gaagctcttg agcttgtcga ggagcgggcc cacgatgtgc tcgtcgattt cctcgatggc
     9421 cttgaccttg gcggcggcgt cgccgttatg gccggcctcg tcgggggcct cgatgtgtac
     9481 cacaatcatg tccatttcat cgagggccgc ggccgccgcc tcgcccttgc ctcggtagtt
     9541 ggtgtcgatg tagccggtgg ctcctgggac ctggatgagc ttccagccga tgctcagagc
     9601 gatgccgcga ataagatcga cggcggtgat ggccgacgcc cgcaggccgt atttctcctg
     9661 gaagctcggt agcctgggtt ttccgccctg accccacagc cagatggacg tggccgggtt
     9721 ctcgcccaga tcacgtctga cgatgttgac gtcgtggttg gcgaggattt cctgcgattt
     9781 ggcgatcagc tcccgcaatt cgtcgctgcc cttgccggtg ggcagatagc cggcgacctc
     9841 ctcgccgggg atatcgtgcg gcggctggca gttgaccttc atcgagatgc cgcccttgac
     9901 caccatcaga tgccggtagc cgacgccgac gtggaacgaa atacgatcgt ttcccagagc
     9961 ggattgcagg tcgttgatga cctgggtggc ctcggcctgg gagatatgtc cggccgagaa
    10021 atcttccatg cgcccgtcga tgatggtgac caggttgcag cggaagacga ggtcgtcagg
    10081 gccaaggctg atgttgttgg caaccgcttc aaggggcgcc cggccggtgt agtgggccct
    10141 gacgtcatag ccgatgacgc tgagcgtggc cacgtcgctg ccgggagtga aatcttcggg
    10201 gatggtgcgg acgaggccgg accgcccggt ggccgcgatc cagttcatgt tcgggatgcg
    10261 ggccgcttcc aaggcggtct tgccgtcgag ctgcgggatt ggctcgtcgg cggccccgtc
    10321 gggcagtatg attgcgtact tcatgtcgga tactcttcgt gttcctcaac cacccggatc
    10381 atgaccggtg actcgcgcac gatatcgagc gaaccgattt ctttcaacgc cgcctggacg
    10441 tggccctcct gggcgacgtc ggtcagcacc acgacggtcg cggttctgtc gccgggcacc
    10501 tgcggcgtct catgctgagt gacggccttg agactgatct gccggtcacc gaaaaccctg
    10561 gtgaacgcgg ccatcacgcc ggggcgatcc acgaccttaa gccggatgta gtagcgcgag
    10621 cgaagctgtt ccatcggcac gtacttgggc gggccggtca cgtcgctgag cactcgaaag
    10681 cgctcgaagg tccggccggc gttgccgatg gcgatgtcga cgatgtcggc cacgacggcg
    10741 ctggcggtcg gcttgctgcc ggctccgcga ccgtagaaga gcgcgtggcc gacccaattg
    10801 ccatagacgc tgacggcgtt gaacgagccg ctggtcgagg ccagcgggtg cttttttgaa
    10861 acgaacgagg gatgaactct caggctgatg ccgtcgtcgg tccgctgagc gatgcccaac
    10921 agcttgcaga cgtagcccag ttccctgccg gcggccaggt cgatcgcatc gagctgatcg
    10981 ataccctcga tgtgcagggc cccgaagtcc acgtcgcagc agaaggccag cgatgccaag
    11041 acggcgagct tgtgggcggt atcggtgccg ttgacgtcaa gcgtcgggtc cagctcggcg
    11101 tagccggcgg cctgggcctc agcgagcgcc attgcgtagg gtttgccgtt ccggctcatc
    11161 tcgctaagga tgtagttgca ggtgccgttg agaatgccgt agatggcgtt gatgcggttg
    11221 gcgatcagac cggtgcgcag cggcagaatc agcgggatgc ccccgccgac gcttgcctca
    11281 aacgccacgc agcaattgtt ccgccgggcg gtcgcgtaga tctccctgcc gtgcttggcc
    11341 aacagggcct tgttggcggt caccacgctc ttgcctgcgt tgagtgccgc cagcacgaag
    11401 tctttggcga cggtcgttcc gccgaccagc tcaaccacca cattgatggt tttgtcgttg
    11461 aagacgtcgg aggcgctggt gctgaccagc ccgaccggga cgttgacctt ggcggcctgg
    11521 gcggggtcct tgtccacgac gcgggccagt tccagccgaa cgccggtctt tcgctcgatt
    11581 tcggcggcct cggcctggat gatgcggacc acgccctgtc cgaccgttcc gcagccgatc
    11641 agtccgattc tgaaaacatc actcattgac cgtgcctcca cacgatctcc cctctccccc
    11701 ttcataaggg agagtcgcag ggggtcgctc tccggcaaac taaagctgtc agcaatcagc
    11761 cgtcagcgtt cagccaagct gggggccgac gagggcgtcg gccctactga ccgatcgatc
    11821 ggacgggcca gaccgtccgg cgaatcgatc aatctagggc tcggtggatt gggatgcaag
    11881 cggcgggctt gcgggcatgc aaacccgatg gaaacaaggg cttgcgcccg gtcggcgggt
    11941 cgacccaggg ggctgaacgg ggggccggag aacggcaact gttgtggggt gtttcaagat
    12001 ccgatcttga aatagcacag agcggaggta tcatcaggct catgatcaag cgcgatcttg
    12061 aacccaggct caagaaggcg gccaagtcgt tcccggcggt cactcttacc gggccccgcc
    12121 agagcggcaa gtcgacgttg tgtcaggccg tgtttgccga tcacccggtc gctaaccttg
    12181 aatcgcccga cgtgcgagct tttgccagtg aagacccccg tgcattcctg gcacagtttc
    12241 ccaaaggggc catcatcgac gaggtgcaac gctgtccgca gattctctcc tacctgcagg
    12301 gcatgatcga cgccgaacgc aaaccgggcc gatggattct gaccggctcg cagaatctgg
    12361 ccctcctgga atcggtcagc cagtcgctgg ccggccgcac ggcggtgatg cagcttcttc
    12421 cgctggtgca cggcgaagtc cgccgcttca aacgccatcc caggtctctg gacgaggtca
    12481 tcttctcggg aagctacccc ggtgttttcg accggaaact cgacccgaag gactggtatg
    12541 catcctacgt agcgacctac gtcgagcgtg atgttcgcac ggtctcgaac gtgggcgacc
    12601 tgaccagctt ccagcggttt ctcggcattt gcgccgggcg gacggcacag atgctcaaca
    12661 tgtcttctct ggcctccgac tgcggcatcg cccagccgac ggtcaaggcg tggttgtcgg
    12721 tgctggagac ttgcttcatc gcttttcgtc ttccggcgtt cagcggcaat atcgccaagc
    12781 ggctggtgaa gatgcccaag ctccatttcc ttgacagcgg cctggtgtgt tggcttctgg
    12841 gcatccgcag cgccgatgaa ctgcggaccc acccgctacg gggcgccgtc ttcgagagct
    12901 gggtcgtgtc ggagatcctc aagcaccgcg tcaaccaggg agaacacggc cacctgttct
    12961 actatcgcga ccagcacggg atcgaggcag atctgattgt cgagcaggga aggaagttga
    13021 ccatcgtgga agccaaggcc gggcaaaccg tcaccggcga catgctcaag agtgcccggc
    13081 gcgtgcaaga gatcctgaaa cccctgcgtc agaccgaggc gatcgtcatc cacggcggct
    13141 cggctgcgca gagccgggac gaggtcaagc ttgtgccgtg gagtacgatt ggcaagctgg
    13201 agtgggtgta gagcaggcat gatctccaca ggacttctga catgaggctt gcgcaatgcg
    13261 tttggccaag ctttcatcaa gcgtgcaaga ggaaggctga acgcaagaag tacacgggcc
    13321 accgcacgcc tcgaactcac caacgaaggt tttgtgtcga taaacaagag ccatgcgagg
    13381 gccgttccgc tctgcgtgcg cattccgcat ctttgcatcc aactcggcaa ctgtgttctt
    13441 ctgctcgtcg cgatcgcagt tcgttgaggc cggccctgct attgaccccc ctcgatcccc
    13501 ccttgccaag gggggaagga aggcatcagg attgggcgtc gcggagtttg cggagctgtg
    13561 tcagcacagc tcggaagtcc ttgggcagcg gggcatcgac gcgcatgcgg gtttgggtgc
    13621 cggggtggac gaaggtcaac gactgggcgt gcaggctcag gcggtcgatg agcgggcgct
    13681 cctcgtgctt ggcgcttggc cgatagttgg gcttgagctg tgacaggaaa aaggccgagc
    13741 tgtccgcata caccgggtcg acgagcagcg gcagccccag ggccttgagg tggacgcgaa
    13801 tctggtgctg acggccggtc agcgggcggc atcgcagcag agtcaccggt ccgagccgct
    13861 cctcgacccg ccaccgggtc accgacggct tgcccttgcg ctggttgatg accatcacgc
    13921 cggtcagacg cggatgcggc gcgagcgggg cgtggatgac gccgctgtcg tcttcggggc
    13981 tgccgctgac cagggcaagg tagtcttttt cgacctcacg tttctggaac tgtaccgaga
    14041 ggaaactctg ggcctgctgt gtcttggcca tcagcaggac gccgctggtg tggcggtcga
    14101 ggcggtgaac gaacagcagg gtagtgtctt tcatccggtc gcgcatcagg atctcgcgaa
    14161 gcgagacgcc tccctgccgg cccggaacgg tcaacatgcc cgcgggtttg ttcaccacca
    14221 ccaggtgctc gtcgtcaaac aggatgtcga tgggttggga catgggaggt tactaggtcg
    14281 tgttgacatt cattgtagcg gttttgacgc ctttgccgat gttgtt
//
LOCUS       DAOTPW010000145        14264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4168_length_14264_cov_5.554719, whole genome shotgun sequence.
ACCESSION   DAOTPW010000145 DAOTPW010000000
VERSION     DAOTPW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14264
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4168_length_14264_cov_5.554719"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="P5316_13575"
     CDS             complement(<1..336)
                     /locus_tag="P5316_13575"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRR86035.1"
                     /translation="MRRSFRGILCILVVLGGCGDEEGAPPPPAAPLPTTGPFAAPIYA
                     GYLKGMKICLDPGHGGRANRKGYKRGPTGLREAEVNLRVALYLREFLEAGGAKVYMTR
                     TRDIYLAEDE"
     gene            complement(389..1189)
                     /locus_tag="P5316_13580"
     CDS             complement(389..1189)
                     /locus_tag="P5316_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRR86036.1"
                     /translation="MPLTAKDIDRHMREIGKYVDWSGTCDTFKAGDASTPIRGIAVSW
                     MSSIANLREAHARGCNLFITHEPTFYSHMDNEKAYDNDTCTKVKRAFLQETGMVVYRC
                     HDVWDRFPNEGILDSWAAFLGLTGEPINAETFWKVMPIKEQPVIVLAHEIGRRVRQLG
                     QDAVLTIGDQNKRVSKVAIGTGAITSAREFHRIGADVGIVTEITWWRDARWALDMGMP
                     LIVVDHTVSEEPGMINLAKYLKRQFPEVKIEYIPTNCPFKVVTAKDED"
     gene            complement(1457..2776)
                     /locus_tag="P5316_13585"
     CDS             complement(1457..2776)
                     /locus_tag="P5316_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86037.1"
                     /translation="MKNDQSVRKSRREFLLEVGTGAVALSASPALVQAEGAVRTVRPS
                     SKQVRIGVVGGGFGAAFQWHLHPNSRVTAVCDLREERIQKLKRVYKCDNGYKDYHEFL
                     KHPELDAVALFTPAPYHVRMAVEALKLGKHVVSAVPAGMSVAELEELLEVVKQTGLKY
                     MMAETSRYRQEVLTCIDWAREGRFGEIFYSESEYHHTGLGPYAYGSSFDCQSCDFIKS
                     VDQVDKDARFSDKLVPTWAYAYPPMLYPTHCTGMIIPVTGERLTEVTAHGWGNDHEML
                     KKNYYDNNPFIHTVALFKTSKGHCSRISIGWNIAAQGTERGLFYGDKMSFIMARPEGS
                     PNTVVEQKIDPNGPFGVFGGEAVSRPFEQPNYLDRLPEPLRIATGHGNSHAFITHEFV
                     SAIIEDRHPEVNVWEAVAYTMPGVIAHQSALAGGACMKIRDYGAAPA"
     gene            complement(3928..4662)
                     /locus_tag="P5316_13590"
     CDS             complement(3928..4662)
                     /locus_tag="P5316_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRR86038.1"
                     /translation="MWDIHWFQVAALIAGALFASAVAAVAGFGGAVLLLPVLTWVFGV
                     RDAVPILTIAQFVGNGSRVWFNRTDIDRRVVGWYSAGAVPLALLGGVIFAAAPLSWLT
                     RGVGAFLVGIVVYRRLGGGRYPRCPAHAFAAVGAGASLLSALVGSTGPVTAPFFLAFG
                     LTRGAYIGTEAAATVITHTFKLAAYGWTSLLSSRVLVTGLLLAPCMFAGSWAGKRLLD
                     RMSERFFIAMIETVMAVAGIMLIAAA"
     gene            complement(4829..5590)
                     /locus_tag="P5316_13595"
     CDS             complement(4829..5590)
                     /locus_tag="P5316_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRR86039.1"
                     /translation="MRILILSDVHANPWALAAVERDAGRFDHVICAGDTVNYGPAPSI
                     AVAWLRQHGSITVRGNHDHAVAFHADPKASPAKQPLAMCMRDWTRAQLGPDDMAWLAR
                     LPLSLQWEIGGVCFAVTHATPLDPLYDYRLTPQQCDAILDDVAAKVQADVLVLGHTHF
                     PLTRRRGSLQIVNPGSLGQPLDGDVRAPYVLWEDGVVHCRRAEYDLEPVICALRQLPA
                     ETIVQDDLVAMLQQATILAKPVIATKEAAYPTRQG"
     regulatory      complement(5702..5770)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6289..7638
                     /locus_tag="P5316_13600"
     CDS             6289..7638
                     /locus_tag="P5316_13600"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRR86040.1"
                     /translation="MMTVSREVYVGALAGLLLATSGCSLESSKTGNARFSDAELLRAG
                     RLTNNEKLYFDKVHGAWSGKLIGLILGQPTEGWGREQIETKAREAGCYPITAYMPAAF
                     DTPHKGFLAGNFDSSPPNDDSDLMLVALLAVRERGIHLTARDLAECWLKYVPSACTAE
                     LVALENFKRNIWPPESATTGNPYAEWIGAQMRVDVWGMIAPGMPRLAAEYATMDASLS
                     HADNGIYAARFIAAAVSLAMVENDVNTVVRRSLELIPADCRYAEAIRDAVAWNRRYSN
                     WQAAWEQLDRKYGFDPDGTRGDQFAEEQYNTGEAPYLWGNWRWVYADVNGAACALALL
                     YGRGDFSRSICLAAMMGYDNDCNSGTVGAILGAMHGHSAIPEQWKKPLNDTYQTGLNL
                     AERRLKISELAKETAGYGRQLVRSCREKRSSRQEHRTAAGTAGSDSSCAGFALPPGI"
     gene            complement(7635..8225)
                     /gene="recR"
                     /locus_tag="P5316_13605"
     CDS             complement(7635..8225)
                     /gene="recR"
                     /locus_tag="P5316_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRR86041.1"
                     /translation="MPASLGRLITELARLPGIGPRSAERIAFHLLRAEKAEAMALADA
                     IRDMKENLRHCSRCYNLTDSDPCPICRDPGRDASLIVVVEQPKEVILLEQTGLLKGHY
                     HVLMGHIAPLERIGPEDLTIDALVRRVKAGGVREVLLATNPTMEGDGTALYIQGLLKP
                     LGVTVSRLARGLAVGSQVEYASRAMLEAAIAGRTPI"
     gene            complement(8347..8691)
                     /locus_tag="P5316_13610"
     CDS             complement(8347..8691)
                     /locus_tag="P5316_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRR86042.1"
                     /translation="MFGALGNLAGLMKQAKSVQENMQRMQETLGEQRYEAEAGAGLVR
                     VVVNGRSELVDIKIDPKAVSDIELLEDMIKGALGAAGRKAQEGMRAEVVKLTGGLNIP
                     GLTDLLGGNSLP"
     gene            complement(8761..10215)
                     /gene="dnaX"
                     /locus_tag="P5316_13615"
     CDS             complement(8761..10215)
                     /gene="dnaX"
                     /locus_tag="P5316_13615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRR86043.1"
                     /translation="MAYTVLARKYRSWTFDELVGQEAVATTLKNAINSRRLHHGYLFC
                     GTRGVGKTSAARILARSLNCLKSDGPTTSPCRECESCVATAEGQDVDVIEIDAASNTG
                     VDNIRELRSNAAYRPARARFKIYIIDEVHMLSTGAFNALLKTLEEPPEHVKFIMATTE
                     PQKVPATIQSRCLRFDFRSIGVEQIAGHLKWILGQEGVQADEAVIRRVARLANGSMRD
                     ALSLLDQLLSMGSDRLTSSMVDDILPVPHDEVLTRLIGQIADRDAAGALVSLDTCLAA
                     GYSLERFAESLADQIRTLMLLKLCGPDTPLAEVPAVARDAMLSLCQRFESDAYVYMIM
                     VLEDLRRSVRFSTMGRALMEAGFVRLASGASYASIQGLLDQLGGGPGGPVEAPPTTPT
                     SVSSRPSQVQPLPRPAVVSGGPTSAASPRIEPVAGGFRRPVPPKPAAPPPRVVSPPSS
                     EDVRAATSDPMVREAMDLFDGTLVHVERLKGPGT"
     gene            complement(10312..10410)
                     /gene="ffs"
                     /locus_tag="P5316_13620"
     ncRNA           complement(10312..10410)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5316_13620"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10471..11655)
                     /locus_tag="P5316_13625"
     CDS             complement(10471..11655)
                     /locus_tag="P5316_13625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR86044.1"
                     /translation="MSSLLSRSATGALILTLLLTVGADAEDTGQSNADDPASIVRADV
                     RLDQPVLQTGQKVWATFTVTNLIDEPVGLQVPGAQLAEMPSTEVGLPLQHVFSARNFG
                     ALTIEEEDRGTVSGETVTQRPVVPVPLITLAPHACLGVRIELTQYYDILRRPGVYKLT
                     WKPYEGVLQSEPAVLTVMAKQRAKMITNLGTITIEFYYAEAPNHVRNFVELVRQGFYD
                     NLTFHRVIPGGIIQGGSPTGDNKGARPDGKTLKAEFSKIPFEAGTVGMARSPNNPDSA
                     SCQFFICLSAQPSFVGNQTAFAKVVGQESFETLKKIASVPTTVKDRPIEPVYIKTISL
                     ENIPERSQATSPSAPDREGAAARAVVRVQNSTRGKTSRAEGSSVVGPNPATSQPAASS
                     DQ"
     gene            complement(11913..12488)
                     /locus_tag="P5316_13630"
     CDS             complement(11913..12488)
                     /locus_tag="P5316_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86045.1"
                     /translation="MLIKIRKRALLLWVVACVVGGMGGCQCGKGDKKSTSPGPSHRAG
                     GEIDGSTKPLRLDFPEQWRASDQEVNAFVESVLTIAAKGDYDGFRQLFAMTHTPPSRQ
                     EFQRAWEAIEGIRVSGLYGDSPEPEKYYLHAVVDRRSPDGRGRTKLDRVVMLFKEAGR
                     WRLGPAPGEVVDVIVRAASRPATWPASLPAS"
     gene            complement(12638..>14264)
                     /locus_tag="P5316_13635"
     CDS             complement(12638..>14264)
                     /locus_tag="P5316_13635"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HRR86046.1"
                     /translation="VPRDELYISKVFVRRQPPPPVDRWVASWKHKAGFATQACRLTDQ
                     AEADLVRSLNAGRRSTGGERGVLLFNPLPWARTGAVRVADRGLPSGEFELLNPSDGTS
                     VPYERRQGLLEFVSPLVPPCGYLYLEVRPVARRLRPGRVAEWSGGNLTLHTADESLQF
                     HTAGGLIRWFDRARSMQWCHTDATHPLGAYLYEMPGGRKLNKFTHQVHTNCWEGTVGY
                     FHRCDYDRMSQYGPLGGRKAVIRPEITSAYARVVVEADCPVRRAAGRRSGDCRGYRTT
                     FTQYRDRRELHINLQLFGKRSSYAAEAGYAVFPINLPKPRIIVERISRLVVPARDLAP
                     GCNAAVMAVHHGVRVEGRYAGMNLYPLDTPLVCFGGPGAYIFDSEHQYDNGVLYAMLF
                     NNCWNTNFALWQEGDFSFDFVLQPTANDTGDGGLSRGGYEFHRPVTARVVSGFSGSPS
                     RSLLMIDSASVQLVVLKPADFGPGVVLRLWNAHWETAQARLVFPEMRRPGSLRRCDLL
                     ERPSGRALRLSPAGEVTVTLKPHEIATYLLDRP"
BASE COUNT         2789 a         4658 c         4177 g         2640 t
ORIGIN      
        1 ctcatcctcg gccagataga tgtcccgggt ccgcgtcatg tacacctttg ccccgccggc
       61 ctccagaaac tcgcggagat acaaagccac cctcaggttc acctccgcct cgcgcagacc
      121 cgtcggcccc cgcttgtatc ccttccggtt ggcacggccg ccatgtcccg gatccaggca
      181 aatcttcatc cccttcaagt aacccgcgta gatcggagcc gcgaaagggc cggtcgtcgg
      241 caacggtgcc gccggcggag gcggagcccc ctcctcatcc ccgcacccgc ccagaacgac
      301 aagaatacag agaatgcctc tgaaagatcg acgcatccat gtgttcacgg acccataccc
      361 cgtttgggct tgatttcgcc ctccgccctt agtcttcatc cttcgccgtc accaccttga
      421 acgggcagtt tgtcggtatg tactcgatct tcacttcggg gaactgccgc ttgaggtact
      481 tggccaggtt gatcatgccc ggctcctcgc tgacggtgtg atcgaccacg atcagcggca
      541 tgcccatgtc caatgcccag cgggcgtcgc gccaccaggt gatctcggtc acgatgccga
      601 catccgcccc gatgcgatga aactcgcggg cgctggtgat cgccccggtg ccgatcgcaa
      661 ccttcgagac tctcttgttc tggtcgccta tggtcaggac ggcatcctgg cccaactgcc
      721 tgacccgacg gccgatctca tgcgccagga cgataaccgg ctgctccttg atcggcatca
      781 ccttccagaa tgtctcggcg ttgatcggct cgcccgtcag cccaaggaac gccgcccaac
      841 tgtcaagaat accctcgttc ggaaagcgat cccacacgtc gtggcaacgg tacaccacca
      901 tccccgtttc ctgcaggaac gcccgtttca ccttcgtgca ggtgtcgttg tcgtaagcct
      961 tctcgttgtc catgtgcgag tagaacgtcg gctcgtgggt gatgaacagg ttgcaccccc
     1021 gggcatgcgc ttcccgcagg ttggcaatcg aactcatcca cgaaaccgcg attcctctga
     1081 tcggcgtcga cgcgtccccg gccttgaacg tatcgcacgt ccccgaccag tccacgtact
     1141 tgccgatctc ccgcatgtgc cgatcaatgt cttttgctgt caacggcatg agattcctcc
     1201 gttcactcgc ccgattccgc tcctggccct cagtatacgc tcaaaccacg acgagccgcc
     1261 aggcaatcgc aggagcgcat cgcgaaaagg gtagcatggc caggcccctg cctgctgggg
     1321 cacggccaag tctttcaacg gaacggtccc tgtaatgtcg gtcgaccctc ttgctcctct
     1381 gcaaggccga ggacaccgat gacgaaatcc caacgcaaca gccggcacag ttctccgttc
     1441 cacggttgct ttctgctcaa gcaggcgctg ctccatagtc gcggattttc atgcacgcgc
     1501 cgccggccaa agccgactgg tgcgcgatga cgccgggcat ggtgtaggcg acggcttccc
     1561 agacattcac ctccggatga cggtcctcga tgatggcgct gacaaactcg tgcgtgatga
     1621 acgcgtgaga gttgccgtgg ccggtcgcga tccgcagtgg ttccggaagt cgatccaggt
     1681 aattcggctg ctcgaaagga cggctcaccg cctcgccgcc gaatacccca aacggcccgt
     1741 tcggatcgat cttctgctcc accacggtat tcggcgaacc ttccggccgg gccatgataa
     1801 aggacatctt gtctccgtaa aacagacctc gctctgttcc ttgagccgcg atgttccagc
     1861 cgatggagat acgggagcaa tgacctttgg aagtcttgaa gagcgcgacc gtgtggataa
     1921 acggattgtt gtcgtagtag ttcttcttca gcatctcatg gtcattcccc cagccgtggg
     1981 ccgttacttc cgtcagccgt tcgccggtga ccggaatgat catgcccgtg cagtgtgttg
     2041 gatagagcat cggcgggtag gcatacgccc aggtcggcac gagcttgtcc gagaatctcg
     2101 cgtccttatc cacttgatca accgatttga tgaagtcaca ggactggcag tcaaagctcg
     2161 atccataggc gtatggcccg agaccggtgt gatgatactc ggattcagag tagaagatct
     2221 ccccgaagcg accttccctc gcccaatcaa tgcaggtgag cacttcctgt cgatagcggc
     2281 tggtctccgc catcatgtac ttcaaaccgg tctgtttcac gacttccagc agttcttcga
     2341 gctctgcgac cgacatcccc gccggcactg cgctgacgac gtgctttccc agtttcagcg
     2401 cctcgaccgc catcctcacg tggtaagggg cgggcgtgaa cagcgccacg gcatccagtt
     2461 caggatgctt gagaaactcg tgataatcct tgtaaccgtt atcgcatttg tagactctct
     2521 tgagcttctg gatccgctcc tcccgcagat cgcacacggc cgtcacccgg ctgttggggt
     2581 gcaggtgcca ttgaaacgcg gcaccgaaac cgccgccgac tacgccgatt cgcacctgct
     2641 tggacgaagg gcgaaccgtc cggacggcgc cttcggcctg gaccagtgca ggtgacgccg
     2701 acaacgcaac cgcccccgtg ccgacttcca gcaggaattc ccgtcgcgat tttcttaccg
     2761 attgatcgtt cttcatttcg gactccccgt gtgacctcgc ccggctacgc cgccgggacc
     2821 tgacccggca ttgtaccgca cgtgccgcgc catttacagc cttccgcagc gacgaacacg
     2881 cgagcggaag acatcggtca agggtgcgac cgtccgttcg gcgacggcta gcgcggcgac
     2941 gagaggatac gaaggcgcgc ggggcggagc ttttcgccgg gtctgattgt cacatgaaac
     3001 cacatacccc cgtcgtgccg ctgccccgcc gagcttgttt tgccggcatt ctgagcacgc
     3061 catatagtta gcatatagag gctataaata cctaattctt ctcaacgtgt taccgtcatc
     3121 ttgtgacagc ctcatgcgtc gaatgactcg cattgttgcg cccggctatc cgcatcacat
     3181 tacctggcgg ggaaaccggc ggcttactgc cggaggagta ccgctgctcg catcgccggc
     3241 acccatgatc ggccggccac ggccgttgcg cttccggcga ggatgggaaa accggcgaag
     3301 attccccggc aacccgatgc cgaccatctc caatcacgcc tttgacgcca cggacgaacc
     3361 ggacgcccgc ctggcaacca ggcctttctc ggcagacccg aggccactct caaccggccc
     3421 ctcggccccc aaaaaccaaa agcagcgtcg aaataggtat ggtgtcctgc ggagtcaaaa
     3481 acctgggtgg gatcgagagg gcgggcaagg tgttcggggc ggatcggata caggaagatc
     3541 atcccgtgtt ttacgcaagt atttatgaca cgaggacttc ggatcttaat tgggttcgtt
     3601 tggcgccggc gagccgtgag gcacattgtt atggaagcgc aacctacctt cggtcggcag
     3661 aaggctgtta ttaacttctc aaagagcgtt ggcgggggcc aggggccgcg gatggttccg
     3721 gtcggcgcgc agggtgtccg ccggtgttgt cgccgaggag aacaccaggc ctgctccgga
     3781 aggggcggtt ggaacggagc ggcgacgggg ctctcagctt caggctcttc cgtcggggct
     3841 ggtgcgcatt gtgcgcgcat gttgtggctg tggccgaagg gtgccttgag agtcctggca
     3901 aacagatttc aacactcctg cacgttctta agccgccgca atgagcatta tgccagctac
     3961 ggccatcacg gtctcgatca ttgcgatgaa aaaccgttcg ctcatccggt ccagcagccg
     4021 cttgcccgcc catgagccgg caaacatgca cggtgccagg agcaggcccg tgaccaggac
     4081 ccggcttgaa agaaggctgg tccagccgta ggcggcgagt ttgaacgtat gcgtgatcac
     4141 cgtcgcggcc gcctcggtgc cgatatacgc ccctcgcgtc agtccaaagg ccaggaagaa
     4201 cggcgccgtg acggggccgg tgctgcccac gagcgccgag agcagcgatg cacctgctcc
     4261 gacggcagcg aacgcgtggg ccgggcagcg aggatatcgg cccccgccca gccggcggta
     4321 caccacgata ccgacgagaa atgcgccgac ccctcgcgtc agccaactga gcggcgcggc
     4381 ggcgaagatc acgccgccga gcagcgccag cggcacggcg ccggccgagt accacccgac
     4441 cacccggcga tcgatgtcgg tgcggttgaa ccagacgcga gagccgttcc cgacgaattg
     4501 tgcgatcgtg aggatcggta ctgcatcgcg cacgccgaac acccaggtaa gcaccggcag
     4561 caatagaact gcgcccccaa acccggccac cgccgcgacg gccgaggcaa agagggcgcc
     4621 ggcgataagt gcggccacct gaaaccagtg aatgtcccac atcggtcgcg ctcacttgtt
     4681 gtcggcggac ggatctcgga taagagacag ccgccttgcc acatgtcacc tcgatttccg
     4741 gcgacccggt tgtacttgca gatccgcggc cgtggctgcc gtccgagtta cggccgtcaa
     4801 cgcgccgaga atcgcatctg cggtgagctt acccctgtcg ggttggatag gcggcctcct
     4861 tcgtcgcaat caccggtttg gctaagatgg ttgcctgctg cagcatggcg acgaggtcgt
     4921 cctgaacgat cgtctctgcc ggcagttgac gcagggcaca tatcacaggt tccaagtcgt
     4981 actccgctct tcggcaatga acgacgccgt cttcccacaa cacgtacggc gcccgaacat
     5041 cgccatccag cggctgtccc agcgagccgg gattcacgat ttgcagggag ccgcggcggc
     5101 gggtcaaggg gaagtgggtg tggcccagga ccagaacatc ggcctggacc ttggcggcca
     5161 cgtcgtcaag gattgcatcg cactgctgag gagtcagtcg gtagtcgtac agcgggtcga
     5221 gcggcgtcgc atgggtcacc gcgaagcaaa cgccaccgat ctcccattgc aggctcaaag
     5281 gcagtcgggc cagccacgcc atgtcgtccg ggccgagttg ggcccgggtc cagtcccgca
     5341 tgcacatggc cagcggctgc ttggccgggc tggctttggg atcagcgtgg aaggcaacgg
     5401 cgtgatcgtg attgccccgc acagtaatcg agccatgctg acgaagccaa gccaccgcga
     5461 tcgagggggc cggcccgtag ttgacggtgt cgccggcgca gatgacatgg tcgaaccgcc
     5521 cggcgtcccg ctccacggcg gcgagcgccc agggattggc atggacatca gaaagaatca
     5581 aaatccgcat ggccgcaagc gcttccaggc tggtcgcagc aacgcgaaga ccccagccag
     5641 tttctgctcc tggtggggtc tgttgcttgc tacgctgagc cggtacggac ccctacccga
     5701 gacacaggta ggagtcatca agcccccacg ggggggccgg ttaagggcgg actccatcgc
     5761 ccgccggatc ttagccgcag ggcttacggg ccgcaaggat gcattcaggg cgcgatgggc
     5821 cgtggcggaa acggagtcgt cgttcgagtt cccttgacgc ggccgctcga ctttcgatgg
     5881 agccgactta ttgtgccaag tggtccatac caggcccttg tgcggcgatt ggccgggcat
     5941 gctcctggct caccggggtt cctgccgtgg acgacggcgg cgatcgttgt accaccgacc
     6001 agtccaatct gaatacgatc ttactatcta ctcaggctct gcagccaagc gtaccgccgg
     6061 ttccgggcga cgctcgcggg tgtccaaggg tctgccctcg ccaacctcac cgatcgtcga
     6121 gcagactttg gtgggcaagc agagcaggtc acagctcagc ctgcgcacat ttcagcgacc
     6181 ctccggatgc aagaccagct tgctttgccc gtggccaaac tgatgatagg gtggtaaggt
     6241 tgccgcattc ccgttgtcgc agacgactca agtactcaga ggaggcacat gatgacggtt
     6301 tcccgcgagg tgtatgtcgg agcactggcc ggtcttctgc tcgcgacctc tggttgttcg
     6361 ctcgaatcca gcaaaacagg aaacgcgcgg ttcagcgacg ccgagttgct tcgggccggg
     6421 cgattgacga acaacgaaaa gctctatttc gacaaagtcc acggtgcctg gtcgggcaag
     6481 ctgatcggac tcatcctcgg ccagccgacc gaaggctggg gccgggagca gatcgaaacc
     6541 aaggccagag aggcgggctg ctatccgata accgcctaca tgcccgccgc cttcgatacg
     6601 ccgcataaag gtttccttgc aggaaacttc gacagctccc cgccgaacga cgactcggac
     6661 ctcatgctgg tcgcattgct ggccgttcgt gaacgcggaa tccatttgac cgcgcgcgac
     6721 cttgccgaat gctggcttaa gtatgtacca tcggcctgca cggctgagct tgttgccctt
     6781 gagaacttca aacgcaacat ctggccgccg gaatccgcaa ccaccggcaa cccctatgcc
     6841 gagtggattg gcgcacagat gcgggtcgac gtctggggca tgatagctcc cggaatgccg
     6901 cgtctggctg cggagtatgc gaccatggac gcgtctctct ctcacgctga caacggcatc
     6961 tacgctgcgc gcttcatcgc ggctgccgtc agcctcgcca tggtcgagaa cgacgtgaac
     7021 accgtcgtgc gtcgttcgct ggagttgatc cctgccgatt gccgctacgc ggaggcgatt
     7081 cgcgatgcag tcgcctggaa ccgccgatac agcaattggc aagccgcctg ggagcagctc
     7141 gatcggaaat acggctttga cccggacggc actcgtggcg accaattcgc cgaggagcag
     7201 tacaacacag gcgaggcccc ctacctgtgg ggcaattggc gatgggtata cgcagacgtc
     7261 aatggagctg cctgcgcact ggctctcttg tacggtcgtg gcgacttttc caggagcatt
     7321 tgcctggcgg ccatgatggg ctacgacaac gactgcaaca gcggcacggt gggcgcgatt
     7381 ctcggagcga tgcacggcca tagcgccatc cccgaacagt ggaagaagcc gctgaatgat
     7441 acatatcaga ccggcttgaa cctggccgag cggagactca aaatcagtga actggccaag
     7501 gagacggccg gctacggtcg gcagcttgtc cggtcgtgcc gggaaaaacg atcctcccgg
     7561 caagaacatc ggaccgctgc cggcacggcc ggatcagatt catcttgtgc cggcttcgcc
     7621 ttgccaccgg gaatctagat gggagttcgt ccggcgatag cggcctcgag catcgctcgc
     7681 gaggcgtatt cgacctgaga accgacagcc aggcccctgg ccaggcgcga gacggtcacg
     7741 cccagcggct tgagaagacc ctggatgtac agcgccgtcc cgtcgccctc catggtcgga
     7801 tttgtcgcca acaaaacctc gcgaacacca cccgccttga cacggcgcac cagcgcatcg
     7861 atcgtcagat cctccggccc aattcgctcc aatggggcaa tgtgacccat gaggacatga
     7921 taatggcctt tcagcaagcc ggtctgctcc aggaggatca cctccttggg ctgctcgacg
     7981 acaacgatca gggaggcatc gcgccccggg tcgcgacaga tcgggcaagg gtctgagtcg
     8041 gtgaggttgt agcaccgcga acagtgccgg aggttctctt tcatgtcgcg aatcgcgtcg
     8101 gccagcgcca tggcctcggc tttttcggcc cgcagcaggt gaaaggcgat acgttcggcg
     8161 ctgcgaggcc caatacctgg cagccgagcc agttcggtga tcagtcgacc gagggaagcc
     8221 ggcatgccgc tgtgttgctc gtccacggcc aatcctcctg aatcgttatt attcaccgcc
     8281 tgcgcgcaat caccggccga caagccgccg gcggtacctc aggaactccg ccccctgctc
     8341 ctcttgttaa ggaagagaat tgccgccgag cagatcggtc aagcccggta tgttcaagcc
     8401 gccggtcagc ttaaccacct cagccctcat gccttcttga gccttacggc cggccgcccc
     8461 caatgccccc ttgatcatgt cctccagaag ctcaatgtcc gacacggcct tggggtcgat
     8521 tttgatatca accagctcag acctcccatt gaccacgacg cgaaccaagc cggccccggc
     8581 ctcggcctcg taccgctgct ccccaagcgt ctcctgcatc ctctgcatgt tttcctgcac
     8641 ggacttcgcc tgcttcatca gaccggccag attgcccaag gctccgaaca ttctcagact
     8701 ccttacacga tcacggcatg cgtatcgccg ccgtcgatgt accccaaacc ccaagattct
     8761 tcacgtgccg ggccccttca agcgctccac atgaaccaga gtcccgtcaa aaaggtccat
     8821 cgcctcgcgc accatcggat cgcttgtggc cgctcgaaca tcttcgctgc tcggcggaga
     8881 aacgacgcgc ggcggcggag cagccggctt ggggggcacc ggccgtcgga aacctccggc
     8941 cacaggttca atccttggcg acgcagccga tgtcgggccg ccggacacga cagccggccg
     9001 cggtaaaggc tgcacttgcg acggcctgga gcttacggaa gtgggcgtcg ttgggggagc
     9061 ctcaaccggc cccccgggtc ccccccccaa ctggtccagc aggccctgga tcgacgcata
     9121 actggccccc gaggccaatc gtacaaaccc cgcctccatg agcgcccgcc ccatggtgct
     9181 gaaccggacg ctgcgtctca aatcctcaag caccataatc atgtagacgt aagcgtccga
     9241 ttcaaagcgc tggcagagcg agagcatcgc atcccgagcc acggccggaa cctcggccag
     9301 cggcgtgtcc ggaccgcaca acttgaggag catcaacgtc cggatctgat cggccagtga
     9361 ctcggcgaat cgttcgaggc tgtagccggc cgccagacaa gtgtcgagcg agacgagtgc
     9421 cccggccgcg tcgcgatcgg cgatctgccc gatcaggcgc gtcagcacct catcgtgagg
     9481 caccggcagg atgtcgtcga ccatcgacga cgtcaggcgg tcgctgccca tcgacagcag
     9541 ttgatccagc agcgacagcg cgtcgcgcat cgagccgttc gccaacctgg cgacccgccg
     9601 gatcacggcc tcatccgcct gaacgccctc ctggccgagt atccacttga ggtgcccggc
     9661 aatctgctcc acgccaatgc tgcgaaaatc gaatcgcaga caacggcttt gaatcgtggc
     9721 cggaaccttc tgcggctcgg tcgtggccat gatgaacttc acatgctccg gaggctcctc
     9781 gagcgtcttg agcaatgcgt tgaacgcgcc tgtgctgagc atgtggactt cgtcgatgat
     9841 gtaaatcttg aagcgggctc tggccggacg atatgcggcg ttgctgcgaa gctcgcgaat
     9901 attgtcgacg ccggtgttgc tggccgcgtc gatctcaatc acatcgacgt cctgcccctc
     9961 ggcggtggca acacatgact cgcactcgcg gcatggactg gtcgtcggcc catcactctt
    10021 cagacaattg agcgacctgg ccagaatacg ggcagcggag gtctttccga caccacgcgt
    10081 gccacagaaa aggtagccgt ggtgcagtcg ccgactgttg atcgcgttct tgagcgtagt
    10141 cgcgacggct tcctgcccga cgagctcgtc gaacgtccaa ctgcggtact ttctagccag
    10201 aaccgtgtaa gccatctcgc aaaccgttgt tctcaacccc gtcgggccgc aaagcgcccg
    10261 tgcaagggcc tccgaaccaa cccccgatta tcggacaaag ccgaccgcat aggagaccag
    10321 gtgagccacg gcacctgaaa cacgaaggct gggctgctac ctctcggtcc tgacccgttg
    10381 gccaacggct ccattgcgtg gaacctggcc cccaatgcga tcggctttgc ctgaaagtcg
    10441 atcccgatgc gttcagccgt ccggcccggc tcattggtcg ctggacgctg ccggctgcga
    10501 ggtcgcaggg tttgggccaa ccaccgagga gccctcggcc cttgaggtct tgcctcgggt
    10561 tgagttctgc accctaacca cggcgcgggc tgccgccccc tctcggtccg gagccgaggg
    10621 agaggtcgcc tgcgaacgct cggggatatt ctccaacgaa atggtcttga tgtaaaccgg
    10681 ctcgatggga cgatctttaa ccgtggtggg cacggaagcg atctttttga gggtctcaaa
    10741 cgattcctgg cccacaacct tggcaaaggc tgtctggttc cccacgaacg acggttgggc
    10801 gctcaggcag atgaagaact ggcaactggc ggagtccggg ttgttgggcg accgtgccat
    10861 gcccaccgtc ccggcctcga acgggatctt gctgaattcc gccttgagcg ttttgccgtc
    10921 cgggcgagca cccttgttgt cgccggtggg gctgcccccc tgaatgatgc cgccgggaat
    10981 cacccgatga aaggtcagat tgtcgtagaa gccttgtcgt accagctcaa caaagttgcg
    11041 gacgtggttg ggggcctccg catagtaaaa ctctatggtg atggtcccga ggttcgtgat
    11101 catcttggcc ctttgcttgg ccatgacggt cagcacggca ggctcagact ggagcacccc
    11161 ctcgtagggc ttccacgtaa gcttgtaaac tccgggccgc ctcaggatgt cgtagtattg
    11221 ggtcaactcg attcgcacgc ccagacaggc atggggcgca agggtaatca gtggtaccgg
    11281 aaccaccggc cgttgcgtga ccgtctcacc cgacacggtt ccccggtcct cctcttcgat
    11341 cgtcaacgcg ccgaaattcc tcgcgctgaa gacatgctgc agcggcagac ccacctccgt
    11401 ggatggcatc tctgccagtt gagcacccgg aacctgaagc ccgacgggct cgtcaatcag
    11461 gttagtcaca gtgaaggtag cccagacctt ctgaccggtc tggagcacgg gctgatcaag
    11521 gcgaacatcg gcccggacaa tcgaggccgg gtcgtcggcg ttactttgcc ccgtatcttc
    11581 cgcatccgca cccacagtca gaagcagggt caggatcaac gccccggtcg cgctccgtga
    11641 caacaagctg ctcatgaatc catcctgtgg aaataccatc gtcccgcgcc gaccagaatc
    11701 ctaagttcct tcaaataaaa cacttaggtc caagacaaaa tccggcatat ggctccagcg
    11761 gcccgcattg gaaacatcca gtcccgatca gccagtctgg accgcccttg catacggccg
    11821 ggatgacgtc ctccggtcct tatacacccc ttctcggcat ccgtcgcatc cttgtatcgc
    11881 aatatctcta gcagcaaggc tttaggcact tctcaggagg ccggaagcga agccggccaa
    11941 gttgccggcc ggcttgcggc tcggacgatg acgtcaacta cctcgccagg tgccgggccc
    12001 aaccgccagc gaccggcctc tttgaaaagc atcacgacac ggtccaactt cgtgcgtccc
    12061 ctgccatcgg ggcttcgacg gtcgaccacc gcgtgcaggt aatacttctc aggctcggga
    12121 ctgtccccat acaagccact gacccggata ccctcgatgg cctcccaggc gcgctggaat
    12181 tcctggcggg acggcggcgt gtgagtcatg gcgaaaagct gccgaaagcc gtcataatca
    12241 cccttggcgg caatggtgag aaccgattcg acgaaggcat tcacctcctg gtcagaggct
    12301 cgccattgct cgggaaagtc aagtctcagc ggcttggtac taccgtcgat ctcgccgccg
    12361 gcccggtgac tgggaccagg agatgttgac ttcttgtcgc ccttgccaca ttggcaaccg
    12421 cccatgccgc cgacaacgca tgcaaccacc cagagcagca gagccctttt gcggatcttg
    12481 atgagcaacg gtcgcctccg aacgcacccg ggttggatgc gagcttgtcg cctgagctcc
    12541 aatccttcat tcgagagcgg catcatactg ctgccggaca cgtgcgccaa ttcatcgagc
    12601 aacctacggc caccctcgcc cattccagct ctgcgtctca tggtcggtcg agcagatagg
    12661 tcgcgatctc atgaggcttc agggtaacgg ttacctcacc ggcaggggac agccgcaaag
    12721 cccggccgct gggacgctca agcaggtcgc aacgacgcag gctgccgggg cgcctcatct
    12781 cggggaaaac cagccgggct tgggcagtct cccaatgggc attccacagc cgcagaacca
    12841 cgcccggtcc aaaatccgcg ggcttgagca cgaccagttg aacactggca gagtcgatca
    12901 tcaacaagga ccgacttggc gacccgctga aacccgaaac gaccctcgcc gtcacaggcc
    12961 gatgaaactc atacccgccc ctgctcaggc cgccgtcccc tgtgtcgttg gccgtcggct
    13021 gcaacacgaa atcaaaagag aaatctccct cctgccagag ggcgaaattc gtgttccagc
    13081 aattgttgaa caacatcgca tacaacacgc cgttgtcgta ctgatgctcg gagtcgaaga
    13141 tgtaagctcc gggcccgccg aaacaaacca gcggggtgtc cagcggatag aggttcattc
    13201 ccgcgtaccg tccctcgacc ctcactccgt gatgcacggc catcactgcc gcgttgcacc
    13261 ccggcgccaa atccctcgca ggcaccacga gccgcgagat ccgctcgacg atgatccgcg
    13321 gcttcggcag gttgatcgga aacacggcat agccggcctc ggccgcataa gaggaccgct
    13381 tgccgaacaa ctggagattg atgtgtagtt cccgccgatc acggtattgc gtgaaagtcg
    13441 tgcgataccc gcgacagtcg ccggaacgtc gccccgcagc ccggcgaacc gggcaatcag
    13501 cttccactac cacgcgggca taggctgagg tgatctccgg ccggatcacg gccttgcggc
    13561 cacccagcgg accatattga ctcatccgat cgtagtcgca acggtgaaag taaccgacgg
    13621 tcccctccca acagttcgtg tgaacctggt gagtaaactt gttgagcttc cgccccccag
    13681 gcatttcata gagataagcc cccaacggat gggtcgcgtc agtgtggcac cattgcatcg
    13741 atcgagcccg atcaaaccaa cgaatcaagc ccccggccgt gtggaactgc agcgattcgt
    13801 cagccgtgtg cagggtcaga ttgcctccgg accactccgc gacccgaccg gggcgcaagc
    13861 ggcgagctac gggccgtacc tccagataca ggtaaccgca cggcggcacg agcggcgaga
    13921 cgaattccag gagtccctgc cgccgctcat atggaacgga cgtgccgtcc gaagggttca
    13981 gtagctcgaa ttcgccggat ggcagaccgc gatcagccac ccggaccgcg cccgttctcg
    14041 cccagggtag tgggttgaac agcagaacgc ctcgctctcc acccgtgctt cgacggccgg
    14101 cattcaggct gcgaaccaga tctgcctccg cctggtctgt caatcggcag gcctgcgtcg
    14161 caaagcccgc cttatgtttc caggaagcca cccagcgatc cacggggggc ggcggctgcc
    14221 ggcgcacgaa caccttgctg atatagagct cgtctcgagg cacg
//
LOCUS       DAOTPW010000146        14089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4237_length_14089_cov_5.056221, whole genome shotgun sequence.
ACCESSION   DAOTPW010000146 DAOTPW010000000
VERSION     DAOTPW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14089
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4237_length_14089_cov_5.056221"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="P5316_13640"
     CDS             complement(<1..250)
                     /locus_tag="P5316_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86047.1"
                     /translation="MTDQVGDRVALPSPRRPLDENTRIGLKALDNLPLFFVGRQREKK
                     AIPLAAGRSLTQRIGDRIAGTTSPKTSELRWQFCAIGGR"
     gene            158..4108
                     /locus_tag="P5316_13645"
     CDS             158..4108
                     /locus_tag="P5316_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRR86048.1"
                     /translation="MVQRLESDPCVLVKGPPGTGKSHTIANLICHLLATGNRVLVTAQ
                     APKALAVLRDLLPKDIRDLCVTALGSSREDQRLLEESVRGILRRKNEWQGRQAAQQEI
                     EEARSQLRELESKLARVERDLRTFREAETHSHELRGGYSGTAAQIARMVSEQEQEFGW
                     FPELSANATFPLDSSESAFLTEAHGCFTADVRAELELEIGAAELPGPDEFQALVARLT
                     AAEEAAQRASRGVDGAKLEFLEAAKTDQLLELRQAVQALTVLALRVGRVLGEVSETIL
                     QDLLASAETRWTRHAADSEALLKDAPRLLEAIGSARVDLPDGIGEDHLRTDVERRLAH
                     FEAGGRKGFSVLAPRVVRETRYIVEGCRVDGKKVAQLEQLRCLRDHLELQRKLRELRD
                     VWGASLTEVSSLRQALSRAEELTAELKELLAFFRSERAALLATVLGSNRTALASDSDR
                     GDWLKAIDALLAYREARDAQQELDRLRETVRRSQHGSNAHPCLRSLENAARASDVGGW
                     RTAWHERERVRQEKQRLARYESLLAKLDGSCAGLAELLRSTAGEPMWQDRVRKLREAW
                     HWAAARAWLRRFSDASAYEARIQNYHRLQQKIEKATENLVSLRAWQAFFERLDEKTVQ
                     SLNAWTRAVDRIGKGTGKHAYRHRRTARQYLMDCVPSIPAWVMPLHKLWDSVEAVPGL
                     FDTVIVDEASQAGVDALALLLLAKRIVVVGDDKQNSPEAVGVPEDDIARLAREHLSAF
                     RFRDEFRPDTSLFDHAERTFGSHITLREHFRCVPEIIHFSNDLCYRDAPLIPLRQAPP
                     ERLRPLCSHFVAEGACEGDGQRIRNRAEADALVETIVKLVDDEAYEGKTMGIIALQGH
                     AQAQLIETLLAQRLDPKVIEERRLRCGEPATFQGDERDVIFLSLVIAPKQEVHYRALT
                     RLPDQRRFNVAMSRARDQVWLFHSVRLHDLGPDDLRRRLVSFFESPQTSAVDALLEDL
                     DRLEREARGGRRLGSQPDPYESWFEVDVALELLRRKFRVRPQVEVAGRRIDLVVEGLD
                     ARIAVECDGDEWHGPEQYEQDMARQRQLERAGWTFVRVRESDFYADRQRAVDAVVEAC
                     GELEIRPVDFVAESQCELPRALAPDRGSPTAVDAEMPVVNEDPERSVAESEMSSSIVG
                     PFTGYSEAPAFPDPREASAANVREFLRQIIERDGPLSRASVYRLYVEGCPDLQRVGKT
                     VRLALNRALGVMLRAGEIVQADELGDGSSEGQVLRIAGAPAVNVRPAGNRDLLEIPPS
                     ELIAVLARKTPRISAAANDEELFRILLKEYGFQRLTQQRREYLSKIVRLFREQA"
     gene            4284..4664
                     /locus_tag="P5316_13650"
     CDS             4284..4664
                     /locus_tag="P5316_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86049.1"
                     /translation="MMKQFTESTVESAALATSVDLVCRPRSNDASVGDLREILRELPK
                     RITGDLRTHKWTNSYTKVLAKFATTGDMRLNVSIEITPDGGVPPHRMEETKAAKRAGS
                     QRRGAGRRVTMAGNSHASRPVVKG"
     gene            4989..5330
                     /locus_tag="P5316_13655"
     CDS             4989..5330
                     /locus_tag="P5316_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86050.1"
                     /translation="MGRQYSAKTFLRNVPNDLLKQYFQKQGIDLGLDWVRLNETEVNP
                     IFVALEALPATTPSQVALQGWVAATANHTEKRRLGAQLDEVFLKIDPLLTGYADQQSN
                     SSRRSGVLAVG"
     gene            5351..6199
                     /locus_tag="P5316_13660"
     CDS             5351..6199
                     /locus_tag="P5316_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86051.1"
                     /translation="MSSMKDEDLLKLCELPADSGADVLALLRTAARLLEQWSREGACA
                     GAAVAMAAAMGRAGHWSVRSLPFGYEGPSIRHEGWIKRDDLALKLTQLGGLICGRRFA
                     ARHADTAIARAVQEYWLREGQHGGWRPGMESGSGWKTVVQAQPVGLGWAREMAESPPT
                     MTIPQVPTAATASTLAAVSSSPPPAACDSFATSNEDGPRQDPALKNADRLTLLTLAMF
                     DPSILASVAQIAEGMDPSERLSERTITPAIRRLVELGLAERPEGERQGARLTLRGRRL
                     ASKIAD"
     gene            6261..6476
                     /locus_tag="P5316_13665"
     CDS             6261..6476
                     /locus_tag="P5316_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86052.1"
                     /translation="METRTLPDKPLPFNKAARCLCVPATWMRDQVNAGRLPALRAGRA
                     ILIHVPTVARLLVQRAQLAGHEEVAHG"
     gene            6469..6696
                     /locus_tag="P5316_13670"
     CDS             6469..6696
                     /locus_tag="P5316_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRR86053.1"
                     /translation="MDNERTSRPAPVSQPPRLALRPREAAKALGIGERLLWSMTNQGL
                     IPHLRLGKAIVYPVAELERWLAERAAKGGRR"
     gene            6693..9143
                     /locus_tag="P5316_13675"
     CDS             6693..9143
                     /locus_tag="P5316_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HRR86054.1"
                     /translation="MNTSTLKEAALAYAEHGWPVVPLHSIEDGACTCKRAGCRSPGKH
                     PRSVHGLKEASSDPTVIETWWRDWPQANVGVVTTGMVVLDIDPRHGGDESLAQLIREH
                     GQLPETVEALSGGGGRHLIFAQSGVVIRSRNNLLAGIDVKADGGYIVAPPSNHISGGT
                     YAWRPGRAPDQRPAADMPTWLLQMVTQPRPSVPPCAPAPSGDELDRLMTRAGQYVAKA
                     AAASEGSRNNAAFNLAGHLHAFQSERGLRLTETQILDLMRTWNHRNCPPLGEDELAKA
                     VRSAARNGTPRPTHQVHISSGSSETPEPCVPPIEPVLPFPVGVMPEPMRSFLEICATA
                     IGCDPSYVAMPMLSTLAAAIGNTRRIQLKRGWTEPAIVWTAIVGESGTLKTPAYRVVL
                     QPVYHLQAKAMKQAAAAQDLYEKALLRYDKELSEWKRRKNAGDPPAKPEPPQAERMHV
                     SDITVEAIAPILLANPRGLLLACDELAAWVGSFDRYAHKGGGIGADAAHWLSMHNGEA
                     ILVDRKSGRPKTIYVPSASVSVTGGIQPGTLNRAMGREHRESGLLARLLLAMPPRRPK
                     RWTEADIPPSTEAALLTIVQRLRELQFTYGENGEPRPVVVALTPAAKEAWITFYNAHA
                     EEQVNLSGDLSAVWSKLEGYAARLALVVHCLRWAAEDPTLADSDNVDERSIAAGVALS
                     QWFGNEAKRIYTVLGESDQDGDRRRLIEWIDLHGGSVTTRDLTHGLRQYRGRTKAAFD
                     ALERLSKAGLGRWVNPKPDPAGGRPSPRFQLADGLAVTKTPAGDSARGGFGSGDGGDS
                     GAAELAADPLAGDDWGEL"
     gene            9691..10932
                     /locus_tag="P5316_13680"
     CDS             9691..10932
                     /locus_tag="P5316_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRR86055.1"
                     /translation="MAKRRSNGQGTLFKRVDNGPWTAAWFDHTGRRRIRSTRTTDRRA
                     AEQILAKFVADAALRRAGVIDPRIDKLAEEAKRDLDGHIAAYEAHLQAGHRADKHVSS
                     TIGYIRAMAADAGWITTADISVEQVNRFAQRLSQHRKRSARTVHAYLTAMKGFTRWLM
                     VSGKIASDPLISVSKPNPKTDRRRERRMLLPEEWEWLRTITAQGPTRYRNTGWERVLI
                     YATAIQTGLRQNELRSLTRGRLYLDHQPPFITVKAGATKNAKDARQYIQPDLAAQLAE
                     HVATQAPASPVFELVGGSEVADMLRADLADARQAWLEAVRNDSVEYQHRLQSDFLKDI
                     NSEGERLDFHALRHTCGAWLALSGAHPKAVQAVMRHSTITLTMDTYGHLFPGQEAETV
                     ARMPQILGNSIFVLRPTGTNP"
     gene            complement(11110..11182)
                     /locus_tag="P5316_13685"
     tRNA            complement(11110..11182)
                     /locus_tag="P5316_13685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11147..11149),aa:Lys,seq:ctt)
     gene            complement(11711..13027)
                     /locus_tag="P5316_13690"
     CDS             complement(11711..13027)
                     /locus_tag="P5316_13690"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRR86056.1"
                     /translation="MLGCDLGTMFKVAVGGGSYQEGLTIHLQGVPPGLCITEEQIYED
                     LLLRKPGQGELTSPRREPDIPIIYNGINAADTMPGFKNAGHTNGTPFVILIPNLDRHF
                     EHIEQYQVTNRTPRPGHASYASYQKYDHWDDAIGAGIFSGRYTSTVVAAGAVAKRILA
                     DKGIEVTAYVREAAGVAMPDMPIEQIRLKATAYRKVRKEHDPIWRFVYQSGRIKPGMR
                     FLEKMPVLSEVERMIGSFPKVEMDEKRIRDEHGIHPSLFCPDLATADEMYRKIVEIKD
                     SGDSSGGVVEVVATGVPAGLGEPVFRKLDGEFGRMLSIGAVKAVEIGAGVKVKNMTGS
                     QCNDQLFADGGKVRFGSNNAGGVTGGLTTGQPIVIRLTVKPTPTISKDQHTVDKVTRE
                     NAVLKAVTRRDPTLVARIWPVAEAFTALILLDHLMMHVGYQGMWRS"
     gene            complement(13202..>14089)
                     /gene="aroC"
                     /locus_tag="P5316_13695"
     CDS             complement(13202..>14089)
                     /gene="aroC"
                     /locus_tag="P5316_13695"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRR86057.1"
                     /translation="THRKEPDTVEIMSGMYEGKTTGVPLMMIVHNSNARPGDYDNIKD
                     LFRPGHADYTYFKKYGIRDYRGGGRASARETVGRVAAGAVAKQLLAKRGVRIVAYTLR
                     AGGINCKRIDLDVIEKNPMRACDLEAAELMVKRVDEIRSQVDSIGGIIECRIMGVPVG
                     LGEPVFDKLDADLAKAILSIPATKGIEFGTGFAAADMTGSRHNDQMTPDGFLSNNAGG
                     ILGGISNGNEIVFRTVIKPTSSIAHQQQTINIRGEPVTIRVTGRHDPCLCPRAVPVVE
                     AMAALVLEDHFKRQEALRA"
BASE COUNT         2632 a         4424 c         4528 g         2505 t
ORIGIN      
        1 atcggccgcc gatcgcgcaa aactgccacc ggagttcgct tgttttggga ctagtggtac
       61 cggcaatccg gtcgccgatc cgctgtgtca agctcctgcc tgcggcgagc gggatcgctt
      121 tcttttcccg ctgccgacca acgaagaaca acggcagatt gtccaacgcc ttgagtccga
      181 tccttgtgtt ctcgtcaagg ggccgccggg gactgggaag agccacacga tcgccaactt
      241 gatctgtcat ctcctcgcca cgggcaaccg cgttctggtg acagcacagg ctcccaaggc
      301 ccttgccgta ctgcgtgatc tgcttcccaa ggacatacgc gacctctgtg tgacggcact
      361 gggctcttcc cgcgaggacc agcgcctctt agaagagagc gttcgcggaa tcctcaggcg
      421 caagaacgag tggcaggggc ggcaggccgc acagcaggaa atcgaagagg caaggagcca
      481 gcttcgcgaa ctcgagagca agctggcccg ggtcgagcgc gacctccgta ccttccgaga
      541 ggctgagacg cactcgcacg agctgcgcgg cggctactcg ggaacggccg cacaaatcgc
      601 gcggatggtg agcgagcagg aacaggagtt cggctggttc ccggagctgt ctgccaatgc
      661 cacgtttccc ctcgactcct cggagagcgc ctttctcacg gaagcccacg gctgcttcac
      721 ggccgacgtc cgcgctgagc tggagctcga gattggggct gccgagcttc ccgggccgga
      781 tgagttccag gcgttggtcg cgcggttgac ggctgcggag gaggcggcgc agcgggcgag
      841 ccgtggggta gacggagcga aactggagtt cctggaggcg gccaagacgg accaactcct
      901 cgagctgcgc caagccgtgc aggcgctgac ggtcctcgcg ctccgggtgg gccgagtctt
      961 gggtgaagtt tcggagacca ttctccagga tctgctggct tctgctgaaa ctcggtggac
     1021 tcgccatgcg gccgattcgg aagccctgct gaaggacgcg ccgcgcctcc tcgaggcgat
     1081 cggctcggca cgggtcgacc tcccggacgg catcggcgaa gaccacctga gaacggacgt
     1141 cgagcggcgc ctggcgcatt tcgaggcagg cggccggaag ggcttctctg ttctcgcccc
     1201 gcgagtcgta cgggagacac gatacatcgt cgagggttgc cgggtcgacg ggaagaaggt
     1261 tgcacagctc gagcagcttc gatgtcttcg cgatcacttg gagctgcagc gcaagctgcg
     1321 cgagttgcga gacgtgtggg gcgcttccct gacggaggta tcgagcctcc ggcaagccct
     1381 ttcccgggcc gaagagctga ccgccgagct gaaagaactg ctggcgtttt tcagatccga
     1441 gcgggcggcc ctgctggcga ccgttctggg gtcgaaccgt accgcccttg cgtccgacag
     1501 tgatcgcggt gattggctca aggctatcga tgcactgctg gcctatcgag aagctcgcga
     1561 cgctcagcaa gagctcgacc ggcttcgcga gaccgtccgt cgctcacagc acggctcgaa
     1621 cgcccatccc tgtttgcgct ccttggagaa cgcagcccgg gcgagcgacg tcggcggctg
     1681 gcgcacggcc tggcacgagc gggagcgtgt ccgacaggag aagcagcggc ttgcgcgcta
     1741 cgagagcctg cttgcaaagc tcgacgggtc gtgcgcgggt ctggcggaac ttctccgcag
     1801 cacagccggc gaaccgatgt ggcaggacag ggtgcgcaag cttcgagaag cgtggcattg
     1861 ggcggctgcc cgcgcgtggc tgcgacgatt ctccgacgcc tcggcctatg aggcgcgtat
     1921 ccaaaactac caccgcctgc agcagaagat cgagaaagca acggaaaacc ttgtttcgct
     1981 tcgggcctgg caggcgttct tcgagcggct cgatgagaaa accgtccaaa gcctgaacgc
     2041 ttggacacgg gctgtggaca ggatcggcaa gggcactgga aagcacgcct atcgccatcg
     2101 gcgtacagcg cggcaatacc tgatggattg cgtcccgagc attccggcgt gggtgatgcc
     2161 gcttcacaag ctctgggact cggtcgaggc ggtgcccggc ctcttcgaca cggtcatcgt
     2221 ggacgaggcg tcgcaggcgg gcgtcgatgc cctggcgctg ctcctgctgg caaagcgcat
     2281 tgtcgtggtg ggcgacgaca agcagaatag cccagaagcg gtcggcgtac ctgaggacga
     2341 cattgcacgc ttggcgcggg agcatctgag cgcgtttcga tttcgggacg agttccgccc
     2401 agatacgagc ctgttcgacc atgcggagcg aacgtttggg agccacatca ccctgcgcga
     2461 gcacttccgc tgcgtgcccg aaatcatcca cttcagcaac gacctatgct accgcgacgc
     2521 gccgctgatc ccgctgcggc aggcacctcc cgaaaggctt cggcctttgt gctcgcattt
     2581 tgtcgccgaa ggtgcgtgtg agggagacgg acagcggatt cggaatcgcg ccgaggccga
     2641 cgccctcgtc gaaaccatcg taaagcttgt agatgacgag gcttatgaag ggaagaccat
     2701 ggggatcatc gcactgcagg gccatgccca ggcgcagctg atcgagaccc ttctcgccca
     2761 acggctcgat ccgaaggtga tcgaggagcg gcgcctgcgg tgcggcgaac ccgccacctt
     2821 ccagggcgac gagcgagacg tcatcttcct ctcactggtg atcgccccga agcaggaggt
     2881 gcattatcga gccctcacgc gcctcccgga ccagcggcgg ttcaacgtgg cgatgagccg
     2941 ggcgagagat caagtgtggc tcttccacag tgtcaggctg cacgatctgg ggcccgatga
     3001 cctgcgccgc cgactggtga gcttcttcga aagtcctcag acttcggcag tcgatgccct
     3061 cctcgaggac ctcgaccgcc tcgagcgcga ggccagggga gggcggcggc tcggcagcca
     3121 gcctgacccg tacgagagct ggttcgaggt tgacgtcgcg cttgagcttt tgcggcggaa
     3181 gttcagagtc cgtccccagg tcgaggtcgc tggtcggcga atcgacctgg tcgtcgaagg
     3241 acttgatgcg cgaatcgctg tcgagtgcga tggtgacgag tggcacggtc cggaacagta
     3301 cgagcaagac atggcgcgcc agcggcaact cgaacgggct gggtggacgt tcgtgcgagt
     3361 gcgcgagtcg gatttttatg cggaccggca gcgagctgta gacgcggtcg tagaggcatg
     3421 cggagagctt gagattcggc ccgtggattt tgtcgccgaa tctcaatgcg agctgccgcg
     3481 agcccttgct ccggaccggg gaagcccgac ggccgtcgat gccgagatgc ccgtcgtcaa
     3541 cgaggatccg gagcggagcg tcgcggagag cgagatgtcg tcctcgatcg tcggcccgtt
     3601 cactggctac tcggaggctc cggcgtttcc agatcctcgg gaagcgtccg cagcgaacgt
     3661 ccgggagttc ctccgacaga tcattgagcg ggacggtccg cttagtcgcg cgtccgtgta
     3721 tcggctctac gttgaaggtt gtccggacct ccaacgagtc ggaaaaaccg tgcggctggc
     3781 gctgaacaga gcgctaggag tgatgctgcg ggctggcgag attgtccagg cagacgagct
     3841 cggcgatggc tcgtcggagg gccaggttct ccgcattgct ggcgcacccg cggtgaacgt
     3901 gaggcccgct ggaaatcgcg acctgctcga gattccaccg tcggaactga tcgccgtcct
     3961 ggcgagaaag actccccgga taagtgcggc cgccaatgat gaggagcttt tccggatcct
     4021 ccttaaggag tatggatttc agcgcttgac acaacagcga agagagtatt tgtcgaagat
     4081 cgtcaggctg ttcagggaac aagcgtgatc gcgatcgccg catcggcgtg gtgtggcaca
     4141 cgcagggctc gggcaagagc ctgacgatat cgtagctgga gcagcgtggg acgttcagga
     4201 tcgaaggagg gatgtatgcg cacctcatga gccacggcgt ggggcacttt cggtattggg
     4261 aggagctata tggagggtcg ctaatgatga agcagttcac cgaatccact gtcgaatccg
     4321 ccgcgcttgc caccagtgtt gacctcgttt gtcgaccgcg atccaacgat gcgtcggttg
     4381 gcgacttgcg cgagatcctg cgggaactgc ccaagcggat tacgggcgac ctgcgtacgc
     4441 acaagtggac gaactcctac acgaaggtgc ttgcgaagtt cgcaacaacg ggggatatga
     4501 ggctcaacgt gagcatcgag atcacccccg acggcggcgt cccgccgcac agaatggaag
     4561 agacgaaggc tgctaagaga gctgggtctc aacgacgagg tgcaggccgg cgagtaacga
     4621 tggcgggcaa ttcccacgca tcgcgcccgg tggtcaaggg ttgattgggt tgccggaagc
     4681 gttgatctcg ctggtgctta cgccgctgcc agccggaact tctgccagaa ggcttcgcga
     4741 aggtggagcc gtagcgatcg cgcccccgct attgacccct ttgcgtgcta ccgcagcgcg
     4801 gacctgctcc cgcattcgct ttccacgcag tttgccgtag cacaggctgt tgacacatac
     4861 ctaacattca gctaacatat tcgttcgatc cgctcggcaa gcggatgcca tccatttcag
     4921 ctcgggcctg gccagggaat cgggcaccag gaaggtgcgg cccgggggtt cattcatacg
     4981 gaggcactat gggccgccaa tactccgcga aaacgtttct gcgcaacgtt ccgaacgacc
     5041 tgctgaaaca gtacttccag aagcagggga tcgacctagg gctcgactgg gtccgtttga
     5101 acgaaaccga ggtcaatccg atcttcgtcg ctctggaggc tttgcccgca accacgccca
     5161 gccaagtggc cttgcagggt tgggtcgcgg cgacggccaa ccacacggag aaaaggcggc
     5221 tcggggccca gttggacgag gtgttcctca agatcgatcc cttgttgacc ggttatgcgg
     5281 atcagcagtc caatagcagc cggcggtcgg gcgtgctcgc agtgggatga ctggatcggg
     5341 tgggcgaaga atgtccagca tgaaagatga ggacttgttg aagctttgtg agctgccagc
     5401 ggactcgggg gcggacgtcc tggctctgtt gcggacagcg gcccggctgc ttgagcagtg
     5461 gtcccgggaa ggcgcttgtg cgggagcagc cgtcgcaatg gcggcggcaa tgggacgggc
     5521 cggacactgg agtgtgcgaa gcctgccctt tgggtatgaa ggcccgagca tcaggcacga
     5581 gggttggatc aagcgtgacg acctggcgct caagctgacc caactgggcg gtctgatctg
     5641 tggacgccgt tttgcagcga ggcacgcgga taccgccatc gcccgcgccg tccaggaata
     5701 ctggctgcgc gaagggcaac acggcggctg gcggccgggc atggagagcg gctccggctg
     5761 gaagacggtt gtacaagcgc agcccgtcgg gctcggctgg gcgagggaaa tggccgagtc
     5821 gcccccgacc atgacaattc cgcaggtacc cacagccgcc acagcgtcta cgctcgcggc
     5881 cgtatcctcc tccccgcctc cggcagcatg cgattcgttc gccacctcaa acgaagatgg
     5941 gccgaggcag gacccggccc tgaagaacgc cgatcgcctg acgctgctga ccctggcgat
     6001 gttcgacccg tcgattctgg cgtccgtggc tcagattgcc gaggggatgg acccgtcaga
     6061 gcggctttct gagcggacca tcacgccagc cattcgcaga cttgtggagc ttggacttgc
     6121 cgaacgaccc gaaggagagc gtcaaggtgc ccgcctgacg ctccggggac gccgccttgc
     6181 gtcgaagatt gccgattaat tgcgcctacg cttccgcctg attacgccct ctcttccgaa
     6241 ccatgctcga tacttcgggc atggaaacga gaacccttcc cgataagccg ttgccgttca
     6301 acaaggccgc ccgctgcctg tgcgtgccgg cgacttggat gcgcgatcaa gtaaacgccg
     6361 gccgactgcc cgcactgcgc gccggccgcg cgatcctgat tcatgtaccc accgtggcga
     6421 gactgttggt ccaacgggca cagctggccg ggcatgagga ggtggctcat ggataatgag
     6481 cggacctcga ggccggcccc ggtgtcccaa ccgccccggc tggcccttcg gcccagagag
     6541 gcggcgaagg ccctgggcat cggcgagcgg ttgctgtggt cgatgacgaa tcaagggctg
     6601 attccgcacc tgcgcttggg caaggcgatc gtgtatcccg tcgcggagct ggaacggtgg
     6661 ctggccgaac gcgcggccaa gggggggcga cggtgaacac atcgacattg aaagaggcgg
     6721 ccttggcgta tgccgaacac ggctggcccg tggtgccgct gcacagcatt gaagacggcg
     6781 cttgcacctg taagcgggcc ggttgcagat cgcccggcaa gcatcctcga agcgtgcatg
     6841 ggctgaagga ggcatccagc gatcccactg tgatcgagac ctggtggcga gactggccgc
     6901 aggccaacgt cggcgttgtg accaccggca tggttgtgct ggacatcgac ccgcggcacg
     6961 gtggcgatga atctctggcc cagctgatcc gtgaacatgg tcaactgcct gaaaccgttg
     7021 aggccctgtc cggaggcggc ggccggcacc tgatctttgc ccaatcaggc gtcgtcatcc
     7081 gcagccggaa taacctgctc gcgggcatcg atgtcaaagc ggacggcggc tacatcgtgg
     7141 ctccgccgtc gaaccacatc agcggcggaa catacgcctg gcgcccggga cgtgctcccg
     7201 atcagcggcc tgccgccgac atgccaacct ggctgctgca gatggtgacc cagccccgac
     7261 catcggtgcc tccgtgcgct cctgctccgt ccggtgacga actcgaccgg ctgatgaccc
     7321 gggccgggca gtacgtcgcc aaggcggccg cggcatcgga aggcagccgc aacaatgccg
     7381 ccttcaacct cgccgggcac ctccacgcct tccagtcgga gaggggcctt cgactgaccg
     7441 agacccagat cctcgatctc atgcggacct ggaaccaccg caactgcccg cctttgggcg
     7501 aggatgaact ggccaaggcc gtccggtcag ccgccaggaa cggcaccccc cgcccaacac
     7561 accaggttca catcagcagc ggctcctctg aaacgcctga gccgtgtgtt ccgccgatcg
     7621 aaccagtcct cccgtttccg gtgggagtga tgcctgagcc gatgcggtct ttcctggaga
     7681 tctgcgccac ggccatcggg tgtgatccgt cctacgtggc catgccgatg ctgtccacgc
     7741 tcgcggcggc gattggcaac acccgacgca tccagctcaa gcgaggatgg accgagccgg
     7801 ccattgtgtg gacggccatc gtcggtgaga gcggcacgct caagacgccc gcttacaggg
     7861 tggtgttaca acccgtgtac cacctgcaag ccaaggcaat gaagcaggcc gccgccgcac
     7921 aggatctcta tgagaaggcg ctgctgcgat acgacaagga gctctccgaa tggaagcgcc
     7981 gcaagaacgc cggagacccg ccggccaagc ccgagccccc gcaggccgaa cgcatgcacg
     8041 tatccgacat caccgtcgag gcgatcgccc cgatcctgct ggctaacccg cgagggttgc
     8101 tgctggcctg tgatgaattg gccgcttggg ttggttcgtt cgaccgatac gcccacaagg
     8161 gcgggggcat cggtgcggat gccgcccact ggctgagcat gcacaacggc gaagccattc
     8221 tggtggatcg caagagtggc aggccgaaga cgatctacgt gccatcggct tccgtgagtg
     8281 tcactggcgg gattcagccc ggcaccctga accgagcgat gggtcgtgaa catcgcgaat
     8341 cggggctgct ggctcggctg ctgctggcca tgcccccgcg gcggcccaaa cgctggacgg
     8401 aggccgatat tccgccgtcc accgaggcgg ccttgttgac gattgtgcag cggctcaggg
     8461 agctgcagtt cacctatggc gagaacggcg agccccgccc ggtggtggta gcgttgacgc
     8521 cggcggccaa ggaagcgtgg ataacgttct acaatgctca tgccgaagag caggttaatc
     8581 tctctggcga cctgtcggcg gtgtggagca agctggaggg gtacgcggcc cggctggcgc
     8641 tggtggtgca ttgcctccgc tgggccgccg aagaccccac actcgctgac tcggacaacg
     8701 tggatgagag gagtatcgcg gccggcgtcg ccctctcgca atggttcggc aacgaggcca
     8761 agcggatcta caccgtcctg ggcgaatcgg atcaggatgg tgaccgccgc cgcctcatcg
     8821 agtggatcga tctgcacggg ggcagtgtca ccactcgcga tctgacacac ggactgcggc
     8881 agtaccgggg caggaccaag gccgcattcg acgccctgga aaggctctcg aaggctggct
     8941 tgggtagatg ggtgaatccc aagcccgatc cagccggggg acgaccttcg ccgcggttcc
     9001 aactggccga tggcttggcc gtcaccaaaa cccctgctgg cgatagcgcc aggggcggtt
     9061 ttggttccgg tgacggtggt gacagtggcg cagcagagct ggcggcagac ccactggccg
     9121 gtgacgactg gggggaactg tgaacacagc ggtcctgttg atggcggagc tgaccgctca
     9181 gggcatcgag ttgcgggccc ggggtgatcg cctgcagttt cggcccgtct cagccctgac
     9241 cgcggaatgg gctgaccgcg tacaacgtca caaacaagag ttgctggccc tgctgagaca
     9301 agcaacgtcc gaaccgctcg aaacaacagc gcagcgggag gcacttcgct ttctgcgagt
     9361 tgcggtcccc aggccggacg gccgaggatg gtgggatcaa tcggcggttc tggatccggc
     9421 cattcgagca gccataggcc cagattccct ggtgaagcgg ccagcccact cggatggacc
     9481 agacccgagc cgtcctggcc ccctggatgc actgactcct gatcaactcc ggcgttacct
     9541 ggccatccgg ccaagctgta cgggtcggcc ctgggaacaa catgcccagg cttggcaggc
     9601 ggcggtctcc ggccgccagg aatcctcgaa tggagtggat tcctctcggg cgttgaggta
     9661 ctcagaacct ctataggcgg gtaaggctcg atggccaagc gacggtccaa cggacaaggc
     9721 acgctcttca aacgggtcga caacggcccc tggactgcgg cctggttcga ccacaccggc
     9781 cggcggcgaa tccggtccac ccgcaccacc gaccgccggg ccgccgagca gatcctggcc
     9841 aagttcgtag ccgacgcggc cctgcgaaga gccggggtca tcgacccgcg gatcgacaag
     9901 ctggccgagg aggccaagcg cgatcttgac ggccatattg ctgcctatga ggcccatctt
     9961 caggccgggc atcgtgctga caagcacgtg tcctcgacga ttgggtacat ccgggccatg
    10021 gccgccgacg caggctggat cacgaccgca gacatttcgg tcgagcaagt caatcgcttt
    10081 gcccagagac tctcccagca ccgaaagcgg tctgcccgca cggtccacgc ctacctgaca
    10141 gccatgaagg gcttcactcg ttggttgatg gtcagtggta agatcgccag cgacccgctg
    10201 atttcggtga gtaagcccaa tcccaagacc gatcgacgca gagagcggag aatgctgctg
    10261 ccggaagagt gggaatggct caggaccatc accgcccaag gacctacccg ctaccgaaac
    10321 accggctggg agcgggtgct gatctacgcg accgccatcc agaccggtct gcgccagaat
    10381 gagttgcgaa gcctgacccg cgggcgactg tacctggatc atcagccccc cttcatcacc
    10441 gtcaaggccg gggcgaccaa aaacgccaag gacgcccgcc agtacatcca gccggatctg
    10501 gccgcccaac tggccgagca tgtggcgacc caagcccccg cctccccggt gttcgagttg
    10561 gtcggcggct cggaggtggc cgacatgctc cgggcggatc tggccgacgc ccgacaggcc
    10621 tggctggaag cggtcaggaa cgactcggtc gagtatcagc atcgtcttca gtcggacttc
    10681 ctcaaagaca tcaattccga aggtgaacga ctcgacttcc acgccctgcg gcacacctgc
    10741 ggagcctggc tggccctgtc cggagctcac cccaaagccg tccaggcggt gatgagacac
    10801 tcgaccatca ccttgaccat ggacacctac ggccacctct tccccggcca ggaggccgag
    10861 accgtcgccc ggatgccaca gatcctgggc aactccatct tcgtcctgcg ccccacgggt
    10921 acaaatccgt gagcccggtc ttcggcggcg cgctagcggt tttgatagca gtcagggcac
    10981 gatatcctgc gagcctgtgc ggctgggtgc gaccagacgg gcaaaacctc cagaccggag
    11041 tcttccctaa gtctttgagc gttgcgggtt tatgcaaaca caaacggccc cgcgcggggc
    11101 cgtgcgaaat gcgtcctcaa ggactcgaac cttgaaccct ctgattaaga gtcagatgct
    11161 ctaccagttg agctaaggac gcaagcgggt cagtgccagg aaccgccccc aggccgtggg
    11221 gtggcccttg ttttgagcct caagatccgt cccgtgctgc tttactaatg accgtcttac
    11281 tcggaaggca gcgcacggca cggacccgca aattcggcca ctggcaacac gaggcgaccg
    11341 gtgcccatta tatcaccctg gccgatgacc gtttcaagca ctcctccaaa gacccagccg
    11401 tagccgacac acaacgcgcc gactgggttg cgacaaagta taacccggcc gaacgcagta
    11461 ctcaagtccc cgccggagcc gccggcgatt tcgcaaacct cgttttcggg tcttgcgccg
    11521 ccctggttta tgatcaatgc gccccaatcg cgattccact tggtgatgct ccgggctctt
    11581 gggtcagagg acgggctgcc gaggctgttg ggcaagcacg gataggaccg gctcgccgac
    11641 aacgccggga gcgtccggct cctcatcaga acatcccatg aaccgcgaaa aacgccggtt
    11701 gcggaatcgc tcaactccgc cacattccct ggtacccgac atgcatcatc aggtgatcga
    11761 gcagaatcaa agcagtgaag gcctcagcca ccggccagat cctcgcgacc agcgtggggt
    11821 cacgacgtgt gacggccttc agaacagcgt tttcccgcgt cactttgtcc accgtgtgct
    11881 ggtccttgct gatggtcggc gtgggcttga ccgtcagccg gatgacgatc ggttggccag
    11941 tggtcaggcc gccggtgacg cccccggcgt tgttgctgcc gaagcgcact ttgccgccgt
    12001 cggcaaatag ttggtcgttg cattgtgagc cggtcatgtt tttgactttg acgccggccc
    12061 cgatctccac tgccttcacc gccccgatgc tgagcatccg accgaactcg ccgtccagct
    12121 tgcggaacac cggttcgccc agcccggcgg gaacacccgt ggccaccacc tcgaccacgc
    12181 caccgctgga gtcgcccgag tccttgattt cgacgatctt gcggtacatc tcgtcggccg
    12241 tcgcaaggtc cggacagaag agactgggat gaatcccgtg ctcgtcacga atcctcttct
    12301 cgtccatctc gaccttgggg aagctgccga tcattctctc gacttcggaa agcaccggca
    12361 tcttctccag gaagcgcata ccaggcttga tccggcccga ctggtagaca aatcgccaga
    12421 tggggtcatg ttccttgcga actttgcggt aagcagttgc cttcagacgt atctgctcga
    12481 tgggcatgtc gggcatggcc acgcccgccg cctctcgaac ataagcggtg acctcaatcc
    12541 ccttgtcggc caggattctc ttggcgaccg ccccggccgc gacgacggtc gaagtgtacc
    12601 gtccgctgaa aatgcctgcc ccgatggcgt catcccagtg gtcgtacttc tgataggagg
    12661 catacgacgc gtggcccggt cttggagtgc gattggtcac ctgatactgc tcgatgtgct
    12721 cgaagtgtcg atcgaggttg gggatcagga tcacaaacgg cgtgccgttg gtgtgaccgg
    12781 cgttcttgaa ccccggcatg gtgtcggccg cgttgatgcc gttgtagatg atcgggatgt
    12841 ccggctcgcg gcgaggcgag gtcagctcgc cctgacctgg cttacggagc aacaggtcct
    12901 catagatctg ctcctccgta atgcacaacc ccggcgggac cccctgaaga tggatggtca
    12961 agccctcttg gtaagaaccg ccccccaccg ctaccttgaa catcgttccc aggtcacaac
    13021 ccagcattgc ttatctcctt gcagttccgt ttctcaggga cactcggacc agctctgact
    13081 accgccgatt gccacccggc gacgctgtca gcagcctgcc cggcttcgga acgagggcga
    13141 gtataggaga ctgctctggt atctccaagg gcatccaaat gccaatttgg cggctcgtcc
    13201 gctatgccct cagcgcctct tgccgcttga aatggtcttc caaaaccaaa gcggccatgg
    13261 cctcaacgac tgggacggca cgggggcaca ggcagggatc atgacggccg gttacccgga
    13321 tggttaccgg ctcgcctctg atgttaatcg tctgctgctg gtgggcgata gaggaggtcg
    13381 gcttgatcac cgtccggaag acgatctcat tgccgttgga gatcccgccc agaataccgc
    13441 cggcattatt gctcagaaaa ccgtccggcg tcatctggtc gttatgccgg ctgccggtca
    13501 tgtcggcagc ggcaaagccg gtcccgaact cgatgccctt ggtcgccggg atggagagga
    13561 tggccttggc caggtcggcg tccagcttgt cgaacaccgg ttcgcccagc cctaccggaa
    13621 cgcccatgat tcggcattcg atgatgccgc cgatgctgtc gacctgagaa cggatctcat
    13681 cgacccgctt gaccatcaac tcagccgcct ccaggtcgca agcacgcatc ggattcttct
    13741 cgatcacgtc cagatcaatc ctcttgcagt taatcccgcc ggcccgcagg gtgtatgcga
    13801 cgatccgcac gccgcgcttg gccagaagct gctttgccac ggccccggct gccacccgtc
    13861 cgaccgtttc gcgcgccgag gcccggcccc caccgcgata gtcgcgaatc ccatatttct
    13921 tgaagtaggt gtagtccgcg tgccctggcc ggaagagatc cttgatgttg tcgtagtctc
    13981 cggggcgggc gttgctgtta tgcacgatca tcatcagcgg cacgccggtg gtcttgccct
    14041 cgtacatgcc ggacataatc tcgacagtgt cgggctcttt acgatgggt
//
LOCUS       DAOTPW010000147        14062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4248_length_14062_cov_6.435140, whole genome shotgun sequence.
ACCESSION   DAOTPW010000147 DAOTPW010000000
VERSION     DAOTPW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_14062_cov_6.435140"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="P5316_13700"
     CDS             complement(<1..251)
                     /locus_tag="P5316_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HRR86058.1"
                     /translation="MIEQLFTALTHAVEGTPIVALAASFVWGILSIILSPCHLASIPL
                     IVGFIDQQGQMTTRRAFGISTLFSVGILITIALIGVITAA"
     gene            complement(301..708)
                     /locus_tag="P5316_13705"
     CDS             complement(301..708)
                     /locus_tag="P5316_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR86059.1"
                     /translation="MNPEVPPAEADRSQSQSNAQDQPAEATTGQDSTAAKKLPRLVDL
                     GAGKCIPCKQMAPILESLKKEYAGRFEVEFIDVWENPNAGNLYRIRMIPTQIFYDSSG
                     KELFRHEGFYGREDILNKWKELGIELADDSQAS"
     gene            complement(862..1542)
                     /locus_tag="P5316_13710"
     CDS             complement(862..1542)
                     /locus_tag="P5316_13710"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HRR86060.1"
                     /translation="MIRPCQQCDGAYPIGMWMSALLICMLVIAWKHWPRMGRIGRVGM
                     IAVCMVVFGGSIATSYWQHTSGSTDGACGTYFTPILPPPATGPSSQPMMGAATVASQP
                     VAESFVFEASPDVGAFYFHRTVRCHSCLQIEEWARQVIETYFGEELAGGLIEWRPVNI
                     DEPGNEHFEKDYRLTTQSLVLVRMIDGRPAEWKNLKSVWEFLGDYAAMTEYVRSELSA
                     FLHDVYGM"
     gene            complement(1588..1830)
                     /locus_tag="P5316_13715"
     CDS             complement(1588..1830)
                     /locus_tag="P5316_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR86061.1"
                     /translation="MKIEILGTGCPKCKTLAENAEKAAKTLGVQYELCKVTQLADIMK
                     RGVMMTPALAIDGEVKSVGKVLSEAEITSLLTSAMP"
     gene            complement(1942..3297)
                     /locus_tag="P5316_13720"
     CDS             complement(1942..3297)
                     /locus_tag="P5316_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008317631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRR86062.1"
                     /translation="MITDWKSESRKLMWGIVVFAACFAMPVDWPRFSKAVFEALALTK
                     WYAQEHVLLCLIPAFFIAGAIAVFVSQASVMKYLGPKAPKVLAYGVASVSGTILAVCS
                     CTVLPLFAGIWRMGAGLGPATAFLYSGPAINVLAIILTARILGLEIGIARTVGAILFS
                     IVIGLIMHLIYRREETAKADAAIAMPEPQVDRPLWQNVLYFGAMVGILVFANWGKPDV
                     TSGFWHGVWSGKWLITSVFAVALALILGVWFNIKWWKLAAVAAATVAAWLVSTSWIAP
                     AIHASAGGHHLPWEAMIPFAVAVVGLSVILAGRDDPSGEWFYQSWTFTKQITPLLLFG
                     VLVAGFLLGRPGEEGMIPDAWVARLVGGNSLWANFFAAIVGAFMYFATLTEVPILRGL
                     MGSGMGKGPALALLLAGPALSLPNMLVIRSIMGTQKTIVFVSLVVVMATISGMVFGAM
                     P"
     gene            complement(3413..3736)
                     /locus_tag="P5316_13725"
     CDS             complement(3413..3736)
                     /locus_tag="P5316_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRR86063.1"
                     /translation="MRTRSKSHYEVRARVAKALAHPSRLLILDVLAEGECCVCKLTPL
                     VGADQSTVSKHLAILKNVGLIEDRKQGLNTYYRLKVRCLEGFWRCIESVLKHNLKEQA
                     AALRA"
     gene            3992..5332
                     /locus_tag="P5316_13730"
     CDS             3992..5332
                     /locus_tag="P5316_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86064.1"
                     /translation="MSRKQDTPISRRSLIKTTGFVAGAAVGLPYILPSGSLAATSGTS
                     ANERLTVAVIGVGGMGTVHLRNLLEFRNEGKVNIAAVCDADERRLAKAVEMAGPGVMP
                     YRDYRYVLQRKDVDAVVIATPDHWHAVQTVHACECGKHVYVEKPASVTIEEGKAMVAA
                     ARRYKRAVQVGAQGRTAKGAHATCTAIRNGIVGKVTKVTCWHYETPADEHPVPDCDPP
                     PELDWDLWLGPLRWRPYNPRYCPGTFRWMMESGGGQIRDRGAHQFSTILWCMNADEQI
                     SFAVEATGTPPTKGLWDCPVTMDVTYTFKNPDWQLIWGQPGEKVGKTEFGNVFWGEKG
                     RLILEWEGAYKAAEPEATNFEVPPGGVVPPRTDEYEDFNMNHKADWFNAIREGTRPAV
                     DIEIGHRVATLCNLGNLSYLLGRRLVWDGERQQVIGDDQANRLLSRPQRYPYHL"
     gene            complement(5475..6593)
                     /locus_tag="P5316_13735"
     CDS             complement(5475..6593)
                     /locus_tag="P5316_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HRR86065.1"
                     /translation="MQRRIAHLPLHGGKAPAWLFKRMHRLAGSVVQLIVADQGPAEML
                     RRLADPLWFQAFGCVLGFDWHSSGLTTVTCGALGEAYRRMGPDLGIHVAGGKGGASRK
                     TPEQIADISAQRSLQGDRLIYSSRMAAKVDSAAVQDGYQIYTHHFLFSDDNEWCVVQQ
                     GMNDATGYARRYHWLGSTVSDFVCEPHNAVASEPADPRLLLNMVAREADSSRLRSSEL
                     ARLQPDKLLREITAGPSLFLPAHHPVTPADINPERLRRILATTFERQPKDFETLLGCP
                     GVGPKTIRSLALLAELIYGTPACRRDITRRWSPADPPTFTYAHGGKDGHPFPVDRSTY
                     DKSIAILESAVRRARIDPGGKDQALFRLSKWMASAGPP"
     gene            complement(6600..7613)
                     /locus_tag="P5316_13740"
     CDS             complement(6600..7613)
                     /locus_tag="P5316_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRR86066.1"
                     /translation="MTAKCRHIHRVPVLPPRAADAHKGTFGRVLIVGGSVGMAGAPAL
                     AGLAALRSGAGLATIAVPESVQPSVAIMCPCATTVPLPETRDGRIDPPAAERRLKKLG
                     LLGVPPDGARPDVVAIGPGVGRGPATYGTQFWQLIEAIRRLGVPAVIDADALNLAPQG
                     GLEAAKGWQHFDHARTVITPHPGELARLLETSIAQIQADREGHAIRTAQAMRANSSPD
                     GSPPVVVLKGAATIVTDGELVYTNNSGNPGMATGGSGDVLTGMIAALIGQGMTPFDAA
                     IAGVYFHGLAGDLAAKRLGQVSMIATDIIDALPEAFQKKPRRGLGQPIPAAQAGTPEH
                     YRA"
     gene            complement(7638..8486)
                     /gene="larB"
                     /locus_tag="P5316_13745"
     CDS             complement(7638..8486)
                     /gene="larB"
                     /locus_tag="P5316_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRR86067.1"
                     /translation="MHESSLRRLLEQVRLGRRSVEQAIGDLRHLPYEDLGFAKIDHHR
                     AIRCGFPEVIYCEGKTIEQVLAVVKHRLAYGGNLLATRASLAMAKAVQKRFPKAHYNP
                     IARTITIRQPSGKASTDLAERKKASSDFVAIVCAGTSDLPVAEEARETCEIMDQPTRQ
                     FYDVGVAGIHRLLAHSAELSQARVVVVCAGMEGALASVVGGLVGCPVIAVPTSVGYGA
                     SFGGLAPLLTMLNSCAAGVAVVNIDNGFGAGYMAALICRAAQRPTAESETSQANEPKP
                     KRTRRR"
     gene            8599..9039
                     /locus_tag="P5316_13750"
     CDS             8599..9039
                     /locus_tag="P5316_13750"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HRR86068.1"
                     /translation="MEVLLPSSSSCRAACSRPADPGTFIPSRPRERQDRRFIWLLIGV
                     WIVNCFDLELTLLAAHQRMLWELNPVVGRVLPFGPKGLTCYKFALLGFGSLVLWRYRR
                     YRVAVVALWIVAIACVGLSLVWYQLYFGAEPTWGGINTSCIVSG"
     gene            complement(9075..10109)
                     /locus_tag="P5316_13755"
     CDS             complement(9075..10109)
                     /locus_tag="P5316_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86069.1"
                     /translation="MMTALARRIGYGLALAVVLAAGCAPIGTGAARQAARSTSATSQP
                     EAKQDKAVDLRCLLIPSSLAQPVRIHVVEHRLTESAETGSTTQDVDLDLHALLRAVKV
                     RDDGAVEATLRFVRIRMKVDAPQSSLAYDSENDKAGKGDQLADLMEAVTDAELTLLVS
                     PDGRLLELRGLDHRWRQAGVIVPPPHLLAVYWFFRDNCMLELVGEALFPVMPAGRAQV
                     GETWEAEIPADIPLAARLNSCLRWSLCSVTDDDPVPAEVWFEAKGTVRLADLPMKDTS
                     PGIRPYLKSGTHNVEAKLCPATGTFQQSSSRLIDVELILTPPSGKERLRTTIHQERRL
                     TAQRGPARRP"
     gene            complement(10233..11786)
                     /locus_tag="P5316_13760"
     CDS             complement(10233..11786)
                     /locus_tag="P5316_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRR86070.1"
                     /translation="MWNERAECVEGNRPSSPFWETLAVGSKIAWQRLADVAREVLCLG
                     PVFTRLDPEPGVHVAGIPVTCRQRTVGAVLTCVLGSEFAEPDRIRRFSHRHDLDAVLL
                     ARLAAETPRRGEDEMLAFADIFSAQVGSMTDRAIARRDVEDLSSQLANAYEQFRLLYR
                     LNTEMTLSHKSTVLFRHMCQDLLAATVIESFAVVLSPDGLSQSEPVCVVAGPLDIKPA
                     DLVRLHDESRRLPTNAGACLVVNNVAERPELAWAAAWLNQFILFPLSSNDRYFGGILA
                     INRRDGQDFGSEEVHFVGAVAQRASTFLENARLYDDLEQLFMGTLHALVSSIDAKDPY
                     TCGHSQRVAWLARHIARLAGVTEEECQRAYLSGLLHDIGKIGVSEAVLRKEGLLTSEE
                     YEEVKRHPGIGANILKGVRQIDDIVPGVLHHHDRYDGGGYPFTLKGDEIPLLGRIVCL
                     ADCFDAITTHRPYRKARPIEDARAELLACAGRQFDPVLTDLFVRENLQDVSREITRSC
                     RSIGNEPVA"
     gene            complement(11963..12358)
                     /locus_tag="P5316_13765"
     CDS             complement(11963..12358)
                     /locus_tag="P5316_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR86071.1"
                     /translation="MIMQQKRILIADDETHILNVLSIKLQNAGFAVIPAEDGLLAWEL
                     ALSDPPDLIITDYQMPGLSGVELCARLRNHPATADIPVIMLTARGFAISDKEVVHGNV
                     RRVIDKPFSPREILAAANELLELVPAASL"
     gene            complement(12355..13893)
                     /locus_tag="P5316_13770"
     CDS             complement(12355..13893)
                     /locus_tag="P5316_13770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR86072.1"
                     /translation="MSACVEATKPSIPVIAERRDPRSPLFSFKRTSWLTLVLLVAVIL
                     VAVIELSGLTEQVSPPDRAAAWKVCTLLVMSVVALLLLGRCIARRSLEHVEIRLRRLM
                     CGDPGIEELDTCVPDVLKPVMGALNEYVALVYWRMRQICDQKRALDEQMRGVEAAKRG
                     IEAIIHSLGEPVLAVDSQCRLTVANAAAGRLFGFQPADARGQAIVDLVKDAHLAALLA
                     DTRALGECRRYRQTECKIQMGESTRTYKMTISTVIDDSGTAQGQVAVFHDVTREREID
                     QLRTDFVSTVSHELRTPLSSIGAYVEMLLDGEAHSEVQRREFYRIIEGETQRLKRLVD
                     NILNISRIEAGALPIHCEPVRVNDVALDVVNALAPQAEEKSLVLDFEAGEALPDLRAD
                     RDLLHQAVMNVVGNSIKYTPHGGRIRVTTALDREASRLTVTVTDNGIGIRTHDLPHVF
                     DKFYRAKEIAGIAGGTGLGLSLVRHIVETVHHGEVCVSSKHGRGTTMSLRFPVVLPRE
                     ECKS"
BASE COUNT         2790 a         4385 c         4269 g         2618 t
ORIGIN      
        1 gccgccgtga tcaccccgat tagggcgatg gtgatcaaga tgccgaccga gaacagcgtg
       61 gagatgccga aggcccgccg ggtggtcatc tgtccctgct ggtcgatgaa accgacgatc
      121 agcggaatgc tcgccaggtg acaggggctc aggatgatgc tgaggatgcc ccagacgaaa
      181 gaggccgcta gagcaacgat cggtgtgccc tcaacggcat gagtcaaggc ggtgaaaagc
      241 tgctcaatca tttgcacgcc ccagttgcgt atgtcccagc aaccgtccga aaaaccgtct
      301 tcagctcgcc tgcgaatcgt ccgccaactc gatacccagt tccttccact tgttgaggat
      361 gtcctcccgg ccgtagaagc cttcgtgtcg gaacagctcc ttgcccgagg aatcgtagaa
      421 gatctgtgtc gggatcatgc gaatccgata cagattgccg gcattgggat tctcccaaac
      481 gtcgatgaac tcgacttcga atcgtccggc gtactccttc ttgagtgact ccaggatcgg
      541 ggccatctgc ttgcagggga tgcacttgcc cgccccgaga tcaaccagcc gcggcagttt
      601 ctttgcggcc gttgagtcct gtccggtcgt tgcctcggcg ggttggtcct gtgcattcga
      661 ctgcgattgc gaccgatccg cttccgcagg cgggacctcc ggattcactt gtgtttgcgg
      721 cggatccgcc gcagggggag atgcctgatt cttgcgtttg agggtcaccg tgacgccgac
      781 cacaaccatg agggccgcga caatgcctaa ccttgcggaa ttgttcatga caaccatctc
      841 tcctgctctg cggtatccaa cctacatccc gtatacgtca tggagaaacg ccgacagttc
      901 gctccgcacg tattctgtca tagcagcgta atcccccagg aattcccaaa cggacttgag
      961 attcttccac tctgccggcc gcccatctat catccgcacc agcaccagcg actgcgtggt
     1021 cagtctatag tccttctcaa aatgttcgtt tcccggctcg tcaatgttga cggggcgcca
     1081 ctcgatcagc ccgccggcaa gctcttcgcc gaagtacgtt tcgatcacct gcctggccca
     1141 ttcctcgatc tgtaaacagg aatggcagcg caccgtccga tggaaataaa aggcacccac
     1201 atccggcgaa gcctcgaaaa cgaaggattc agcgaccggc tgcgaagcaa ccgttgcagc
     1261 gcccatcatc ggctgactgc tcggtcctgt ggcaggaggc ggaagaatgg gtgtgaaata
     1321 cgtgccgcat gctccgtctg ttgaaccgct cgtatgctgc caatagctgg tcgcgattga
     1381 cccgccgaag accaccatgc agaccgcgat catgccgacg cgacctatcc gtcccatacg
     1441 cggccaatgc ttccacgcga tcaccagcat gcaaatcagc agcgccgaca tccacatgcc
     1501 gatcgggtac gcgccatcac actgttggca gggccggatc atcagaccaa cctcaacagg
     1561 ccctcaaggc aaaagccgcg acgaccgtca gggcatcgca ctcgtcagaa gcgaggtgat
     1621 ctcggcctcc gagagaacct tgcccaccga cttcacctcg ccgtcgatcg ccagggccgg
     1681 ggtcatcatc accccgcgtt tcatgatgtc cgcaagctgc gtcaccttgc acagttcgta
     1741 ctgaacgcca agggtcttgg cggctttctc cgcattttcc gccagcgtct tgcacttggg
     1801 gcaaccggtg ccaagaatct cgatcttcat atcaaacctc cagtacgatt gagcgtgggg
     1861 cggcaggcct ttgccctgct tcgtccatgg gacttcgcag gactccgacc gtgccctacc
     1921 ttgttcacga tctttctccc gctacggcat cgcgccgaaa accattcccg agatcgtggc
     1981 catgaccacg accagcgata cgaacacgat cgtcttttgc gtccccatga tgctgcgaat
     2041 gaccagcatg ttcggcaaac tcagggccgg acctgccagg aggagcgcca gggccggtcc
     2101 cttgcccatg ccggagccca tgagcccccg caggatcggc acctcggtca gcgtcgcgaa
     2161 gtacatgaac gcgccgacga tcgccgcaaa gaagttggcc cagagcgaat taccgcccac
     2221 cagacgcgcc acccaggcgt cgggaatcat gccctcttca cccggccgac ccagcagaaa
     2281 gcccgccacc aacacgccga acaacaacag cggagtgatc tgcttcgtga aagtccacga
     2341 ttgatagaac cattcgccgg acggatcgtc ccggccggcc agaatgaccg acaggccgac
     2401 caccgccacg gcaaagggaa tcatggcttc ccatggaaga tgatgaccgc cggcggaagc
     2461 atgaatggca ggtgcgatcc aggaagtgct gaccagccag gcggcaaccg ttgccgctgc
     2521 caccgcagcc agcttccacc atttgatgtt gaaccagaca ccgagaatga gagccaaggc
     2581 aacggcgaaa accgaagtga tcagccactt gccggaccac accccatgcc agaaacccga
     2641 ggtcacatcc ggcttgcccc agttggcgaa caccaggatg ccgaccatgg caccaaagta
     2701 caaaacgttc tgccacaagg gccgatcgac ctgcggttcc ggcatcgcta tggccgcatc
     2761 cgccttggcc gtctcctctc tgcggtaaat caggtgcatg atcagaccga tcacaatgct
     2821 gaagagaatc gccccgacgg tacgggcaat accgatctca aggcccagaa tgcgggccgt
     2881 caggatgatg gccaatacgt tgatggccgg acccgaatag aggaatgccg tcgccggacc
     2941 aagacctgct cccatccgcc agatccccgc aaacagcggt agcaccgtac acgaacaaac
     3001 cgccagaatc gtgccgctta cagacgccac gccataggcc agcaccttgg gcgctttggg
     3061 gcccaggtac ttcatgaccg acgcctgact gacgaaaaca gcaatcgctc cggcaatgaa
     3121 gaaagcgggg atcaggcaga gcaggacgtg ctcctgggca taccatttgg tcagtgccag
     3181 ggcttcaaac acggccttgc tgaatcgcgg ccagtccacc ggcatcgcga agcaggccgc
     3241 gaaaacgacg ataccccaca tcagcttcct ggactcggac ttccagtcgg tgatcattcg
     3301 acacaatctc gcggcgtaga ctccgttcgt cgaacttcgg atgcgggcaa ttccatgcct
     3361 atatggtcag attgccatga acccggcgta acaaaaagcg gtcccggccg cctcacgccc
     3421 gcagcgccgc ggcctgttcc ttcaggttgt gtttcaatac cgactcgatg catctccaga
     3481 aaccttccag acagcggact ttcaaacgat agtaggtatt gaggccctgc ttgcggtcct
     3541 cgatcagtcc gacgttcttg aggatggcca gatgtttgga caccgtggac tgatccgccc
     3601 cgaccagagg cgtcaactta cagacacaac attccccctc ggccaagacg tcgagaatca
     3661 acaagcggct gggatgagcc aaggccttag caacccgggc tcgaacctca taatgtgatt
     3721 tggatctggt cctcatggct tcatggcaat tatgccatat agttcggcaa agtcaaggcc
     3781 gaacttgcgt taccgggccc ggtttgccac tggggcgcct gatgggggga ttgaacccct
     3841 cagctctccg cgaatccgga gagggctgcc cgaagacagg caagcccgaa gaccccgggt
     3901 tgaaacagcc caccgggagg tttacatgga cggggcagat acccgtcgtt aagatccttc
     3961 cagcaccccc gcttcctggg aggtctcagc catgagccga aagcaagaca ccccgatatc
     4021 acgacgcagc ttgatcaaga ccaccggttt tgtcgccggc gccgccgttg gcttgcccta
     4081 cattcttccc tcaggctcgc tggcggccac ctcgggcaca tcggcgaatg aacgtctgac
     4141 agtagccgtc atcggcgtcg ggggcatggg tacggttcac ctcaggaacc tgctggagtt
     4201 caggaacgag ggcaaggtca acatcgcggc cgtctgcgac gcggacgaga ggcgacttgc
     4261 caaggcggtc gagatggccg ggccgggagt catgccctac cgcgactacc gctatgtcct
     4321 ccaacgcaag gacgtcgacg cggtcgtgat cgcgacccca gaccactggc acgccgtgca
     4381 gaccgtgcac gcgtgcgaat gtggcaagca cgtgtacgtc gagaagccgg cgtcggtcac
     4441 aatcgaagaa ggcaaggcca tggtggcagc ggcccggcgg tataagcggg ccgtccaggt
     4501 aggtgcccag ggacgaactg ccaagggtgc tcacgccacg tgcacggcca tccgcaacgg
     4561 cattgtcggc aaggtcacga aggtgacatg ctggcattac gaaacacccg cggacgagca
     4621 cccggtaccg gattgcgacc cgccgcccga gttggactgg gatctgtggc tggggccgct
     4681 tcgatggcgg ccttacaacc cgcgatactg cccggggacg ttccgctgga tgatggagtc
     4741 gggcggcggg cagatccgcg atcgcggggc acatcagttc agcaccatcc tgtggtgcat
     4801 gaacgccgac gagcagatct cgttcgccgt cgaggccacg ggtaccccgc cgaccaaggg
     4861 gctttgggac tgcccggtca cgatggatgt cacctacaca ttcaagaatc ccgactggca
     4921 attgatctgg gggcagccgg gcgagaaggt cggcaagacc gaattcggaa acgtcttctg
     4981 gggcgagaaa gggcgactga ttctcgagtg ggaaggcgct tacaaagctg cggaacctga
     5041 agccaccaac ttcgaggtac cgcccggcgg cgtcgttccg ccacgaacgg acgaatacga
     5101 agacttcaac atgaatcata aggccgactg gttcaacgcc atccgcgaag gcacacggcc
     5161 ggccgtcgac atcgagatcg gccaccgtgt ggcgacgctc tgcaatctcg gcaatctgtc
     5221 atacctgctg ggacgcagac tggtctggga cggcgagcgg caacaggtga tcggcgacga
     5281 ccaggccaac cgcctgctca gccggccgca acgatacccc tatcacctgt agacgcaggt
     5341 caccagacat gcttgcctgg aacctgacaa cccaggatcg gtggaccccg cacaacggtg
     5401 gttttctgac ctttcggggc cttggatgac accggataac cgacgctgat cgaagaacgc
     5461 ggcaatcacg tccgtcacgg ggggccggct gatgccatcc acttcgacaa tctgaacaac
     5521 gcttgatcct tcccgccagg gtcgatcctg gcccggcgaa ccgcggattc gagaatggcg
     5581 atggatttgt cgtaagtaga gcggtccaca gggaaaggat ggccgtcctt gccgccgtgg
     5641 gcgtaggtga aagtgggcgg atcggcgggc gaccagcggc gggtaatgtc gcggcggcag
     5701 gcgggggtgc cgtagatcaa ctcggccagc agggcaaggc tgcgaatcgt cttcggtccg
     5761 acacccgggc agccgagcag cgtctcgaag tctttcggtt gtctctcgaa agtagttgcc
     5821 agaatccgcc gcagccgctc ggggttgatg tcggccggcg tcaccgggtg atgcgcgggc
     5881 aggaacaatg atgggccggc agtgatctcg cgcagcagct tatcgggctg aagccgggcc
     5941 agctcactgg agcgcaaccg cgaggaatct gcctcgcgag ccaccatgtt gagcaggagc
     6001 cgcggatcgg ccggctcgga ggccacagcg ttgtgcggtt cgcagacgaa atcgctcacc
     6061 gtgctgccca gccagtggta tcgccgggcg tagccggttg cgtcgttcat gccttgctgg
     6121 acaacgcacc actcgttgtc gtcgctgaac aggaagtggt gcgtgtagat ctgatatccg
     6181 tcctggaccg cggcgctgtc cactttggcg gccatgcgcg atgagtagat caggcggtca
     6241 ccttgcagag atcgctgggc ggagatgtcc gcgatctgct cgggcgtttt gcgcgaggcg
     6301 ccgcccttgc cgccggcgac gtggatgccg aggtccggtc ccatccgccg ataggcttcg
     6361 cccaaggcgc cgcaggtgac cgtggtcagg ccgctcgaat gccaatcgaa cccgaggacg
     6421 cagccgaacg cctggaacca aagcgggtct gccagccgcc gcaacatctc ggccgggccc
     6481 tgatcggcga caatgagctg tacgacggaa ccggccagcc ggtgcattcg cttgaacagc
     6541 caagccgggg ccttgccacc gtgaagcggg agatgtgcga tgcggcgttg catgggttct
     6601 cacgcccgat agtgttccgg ggtaccggcc tgcgccgccg gtattggttg gccgaggccg
     6661 cgacgtggct ttttctgaaa cgcctcgggc agggcatcga tgatgtccgt tgcgatcatc
     6721 gagacttggc cgagtctctt ggccgccaag tcgccagcca acccgtggaa gtacacaccg
     6781 gcgatggctg cgtcgaacgg ggtcatcccc tgtccgataa gtgcggcgat catccccgtt
     6841 agcacgtcac cgctgccacc cgtggccatg ccggggttgc cgctgttatt cgtgtagacc
     6901 aactcaccgt cagtgacaat cgttgcggcc cccttgagaa cgacaaccgg cggactgccg
     6961 tcagggctgc tgttcgcacg catggcctgc gcggtacgga tggcgtggcc ctcccggtca
     7021 gcctggattt gagcgattga ggtttcgagc agccgagcca gctcaccagg gtgaggggta
     7081 atgaccgtcc gagcatggtc gaagtgttgc cagcccttgg ccgcctctag gccaccttgg
     7141 ggggcgaggt tcagggcgtc ggcatcgata accgccggga cacccagtcg ccgaatggcc
     7201 tcaatgagct gccagaactg ggtgccatag gtagccggtc ctcgcccgac acccgggccg
     7261 atcgccacca cgtccggccg ggcaccgtcc ggcggcacac ccagcaggcc cagcttcttg
     7321 agcctacgtt cggcagcggg gggatcgata cggccgtcgc gggtctcggg caagggaacg
     7381 gtcgtggcgc agggacacat aatggccacg gaaggctgga cggactccgg tacagcgatg
     7441 gtcgccaggc cggccccgct tcgcaacgct gccaagccgg ccaaggcggg ggcgccggcc
     7501 atgccaacgc ttcccccaac gataagaacc cgtccaaaag tgcccttatg ggcgtctgca
     7561 gcacggggag ggagtaccgg gacacggtga atgtgtctgc acttggctgt catgtgtcac
     7621 tctctctgca caaccgatca tcgcctgcgt gttcgtttcg gcttgggctc attcgcctgg
     7681 cttgtctcgc tctcggcggt cggccgctgt gctgcacgac atatcagggc ggccatgtag
     7741 ccggcgccga agccgttgtc gatgttcaca acggccacgc cggcggcgca actgttcagc
     7801 atggtaagca gcggagccag accgccgaag ctggccccat aaccgacgct ggttggtacg
     7861 gcgatgaccg gacaaccgac cagcccgcca acaacactgg ccaaagctcc ttccataccg
     7921 gcacatacga caacgacgcg ggcttggctc agctcggcgg aatgggccaa caggcggtgg
     7981 atgcccgcca cgccgacgtc ataaaactgc cgagtcggct gatccatgat ttcgcaagtt
     8041 tcgcgggcct cctcggcgac gggcaggtcg gaggttccgg cgcaaacgat ggccacgaaa
     8101 tcggacgaag cttttttccg ctcggcaagg tcggtcgagg ccttccctga gggctgcctg
     8161 atggtgatcg tccgggcaat cggattgtag tgggcctttg ggaaccgctt ttggaccgct
     8221 ttggccattg cgaggctggc gcgggtggcc agaaggttgc cgccataagc cagacggtgt
     8281 ttgacgaccg ccaaaacctg ttcgatcgtc ttgccctcac agtagatcac ctcaggaaag
     8341 ccgcagcgaa tcgcccgatg atggtcgatc ttcgcgaagc ccagatcctc atagggcaag
     8401 tgcctgaggt ctccgatcgc ctgttccacg gatcgccggc caagacgtac ctgttcaagc
     8461 aagcgtctga gtgatgattc gtgcatgccc gccatcatag agtcgctcgg ggcaaaacgc
     8521 caccgtgcga acggccgcgt tttcagaatc tgtcgcgtgt tcataaaagt cttgttcgtc
     8581 cgttccgata caattggcat ggaggtctta ctcccctcat cttcgtcctg ccgagcggca
     8641 tgttcacgac ccgctgatcc gggcacgttc attccctcgc gtccgcgcga gcggcaggat
     8701 cgccggttca tctggctcct gatcggagtc tggatcgtca actgctttga ccttgaactg
     8761 actctgctcg ccgcccacca acgcatgctt tgggagttaa accccgtggt cggcagagtg
     8821 ctgcccttcg gtccgaaggg gctgacctgt tacaagttcg cgttgctcgg attcggcagc
     8881 ctggttctgt ggcggtaccg gcggtatcgc gttgcggtcg tcgccttgtg gatcgtcgcg
     8941 atcgcctgtg tgggactgtc actggtgtgg taccagcttt atttcggggc cgagccaacc
     9001 tggggcggca tcaacacaag ctgcatcgta tcaggctgat cgatactcac ttccgggacg
     9061 cgattcctga aacctcaggg gcgtcgggct ggaccccgtt gtgcggtcag ccgtcgctcc
     9121 tggtgaatcg tcgttcgcag tctttccttt ccactggggg gcgtgaggat gagttccaca
     9181 tcgatgaggc ggctgctgct ttgttggaac gtacccgtcg caggacagag cttcgcctcc
     9241 acattgtgag tgccagactt aagataaggg cgaatacccg gcgacgtatc tttcatcggt
     9301 aagtcggcca accgcactgt tcctttggct tcgaaccaca cttcggccgg taccggatca
     9361 tcgtcggtga cggaacagag gctccagcgc agacatgagt tcagccgcgc ggccagcggg
     9421 atgtcagcgg ggatttcggc ttcccaggtt tcgccgacct gcgcccgacc ggcaggcatg
     9481 accgggaaca gcgcttcgcc gacgagttca agcatgcaat tgtcacggaa gaaccagtag
     9541 acggcgagca ggtgcggcgg cgggacaatg acgccggcct gcctccatct gtggtcgagt
     9601 ccgcgaagct cgagcaaacg accgtcggga ctgaccagca gagtcaactc agcgtcggtc
     9661 acagcctcca tgagatccgc cagttgatca cccttgccgg ccttgtcgtt ttccgaatcg
     9721 taagccagcg acgattgagg cgcgtcgacc ttcatccgga tccgcacgaa tcgcagggtt
     9781 gcttcgacgg cgccgtcgtc tcggaccttg accgccctca gcaaggcgtg caaatcgaga
     9841 tcgacatctt gtgtcgtcga gccggtctca gcggattccg tcagccggtg ctcaaccacg
     9901 tggatgcgga caggctgcgc gagcgacgaa ggaatcaaga ggcaacggag gtcgacggcc
     9961 ttgtcttgct tggcttcggg ttgagaagtc gcgcttgtcg agcgcgcagc ttgtcgggca
    10021 gcgcccgttc cgataggtgc acagccggcg gccagcacaa cggccaatgc cagtccgtaa
    10081 ccaatcctcc gggctaacgc cgtcatcatg atgttcctgc atcgctcgga atcagcctac
    10141 ggctgatccg ctcaagccga ctccggtact ggattgtcgt gggagtcttc tcagttggca
    10201 agggcggcgg attcgatcaa cgcagagaat catcaggcga ccggttcatt gccaatgctg
    10261 cggcacgaac gggtaatttc gcgactgacg tcctggaggt tttcacgaac gaagagatcg
    10321 gtaagaaccg ggtcaaattg acgcccggcg caggcgagca gttcagcccg ggcgtcctcg
    10381 atcgggcggg ctttccgata gggtcggtgg gtggtaatgg catcaaagca atcggccagg
    10441 cacacgattc gtccgagcag cgggatttcg tcgcccttca gggtgaaggg atatcctccc
    10501 ccgtcgtagc ggtcgtgatg gtgcagaacg cccgggacga tgtcatcaat ctgccgcacg
    10561 cccttgagaa tattggcgcc gattcccgga tggcgtttga cttcctcgta ctcttccgac
    10621 gtcagcaggc cctccttgcg caacaccgcc tccgagacgc cgatcttacc gatgtcatgc
    10681 aggagaccgc tgagataggc ccgctggcat tcctcctccg tgacaccggc cagccgggcg
    10741 atgtggcgag caagccacgc gacgcgctgg gaatggccgc aggtgtaggg atcctttgcg
    10801 tcgatactgc tgaccagggc gtgcagcgtg cccatgaaga gctgctcgag atcgtcgtat
    10861 aggcgggcgt tttccaggaa agtcgatgcc cgttgggcca ccgctccgac aaagtggacc
    10921 tcctccgagc cgaagtcttg cccgtctctc cggttgatcg ccagaatacc gccaaagtac
    10981 cggtcgttgc tcgacagcgg gaaaaggatg aactgattga gccacgcggc cgcccaagcg
    11041 agttcaggac gctccgcaac gttgttgacg acgagacatg cgccggcgtt cgtgggcaga
    11101 cggcgcgatt catcatgcag tcggacaaga tcggccggct tgatatccag cgggccggca
    11161 acgacgcaga cgggttcact ttgggacagt ccgtccgggc tcaagacgac ggcaaagctc
    11221 tcaatgacgg tggccgccag cagatcctgg cacatatgcc tgaacagaac cgtcgatttg
    11281 tgcgacaagg tcatttcagt attgaggcga tagagcaggc ggaactgctc ataggcgttt
    11341 gcaagttggg aggagaggtc ctcgacgtcc cgcctggcga tcgcgcgatc cgtcatcgac
    11401 ccgacctggg cggagaagat gtccgcgaag gcgagcattt cgtcctcgcc gcgacggggt
    11461 gtctcggcag ccaggcgggc aagcaggaca gcgtccagat cgtgacggtg cgagaaccgt
    11521 ctgatgcgat ccggttccgc aaactcggac cccagaacgc atgtcaggac tgctcccacg
    11581 gttctctgcc ggcaggttac cggaatcccg gcgacgtgaa cgccgggctc cggatccagc
    11641 cgggtgaata ccggaccaag gcacaacact tcgcgagcaa cgtccgcaag tcgttgccag
    11701 gcgatcttcg agccgacggc aagcgtctcc cagaaaggcg atgacggacg gtttccttca
    11761 acgcattcgg ctcgttcatt ccacaaggag acccagagcc ccagccgaga ccaggactca
    11821 gaccacgggc caaagcattc gccgagcagg aaaccgggtt catgtgtcgt cttgttgatc
    11881 aggtccgtgt cctttccctc atcatgcggc catggaaagc agcgttgcga actcgcgggt
    11941 ttccgcttgt tcgggtccgg tctcaaaggc ttgcggccgg caccagctcc agcaattcgt
    12001 tcgccgccgc caggatctcg cgaggactga acggcttgtc gatcaccctg cggacgtttc
    12061 cgtggaccac ctccttgtcg gatatcgcaa agcctcgagc tgtgagcatg atcaccggta
    12121 tatcggccgt cgcggggtga ttgcgaagtc tggcgcacag ctccactccg ctcaggcccg
    12181 gcatctggta gtcggtgatg atcagatccg gcgggtctga caacgccagc tcccaggcca
    12241 gaagaccgtc ctcggccggg atcaccgcga aacctgcgtt ctgaagcttg atcgacagca
    12301 cgttgagtat gtgcgtctcg tcgtcggcga tgagaatgcg cttttgttgc atgatcatga
    12361 cttgcactcc tcacgcggaa gcaccacggg aaaccgcagt gacatggtcg tgccccgtcc
    12421 gtgcttggag gaaacgcaga cttcgccgtg gtggacggtt tcgacaatgt gtctgaccag
    12481 gctgagcccc agcccggtac caccggcaat gccggcgatc tcctttgccc ggtagaactt
    12541 gtcgaagacg tgcggcaggt cgtgtgtgcg aataccaatg ccgttgtcgg tcacggtcac
    12601 ggtcagtcgc gaggcctcgc gatccagggc tgtcgtgacg cgaatccggc cgccgtgcgg
    12661 agtgtacttg atcgagttgc cgaccacgtt catgaccgcc tgatgcagca ggtcgcggtc
    12721 ggccctcagg tcgggcagcg cctcgccggc ttcgaagtcc agcacgagcg atttctcctc
    12781 cgcctgcggt gccagagcat tgaccacgtc gagggccaca tcgttgactc gcacgggctc
    12841 gcaatggatt ggcagggccc ctgcctcgat ccgcgagatg ttaaggatgt tgtcgaccag
    12901 acgcttcaaa cgctgcgtct cgccctcgat gatccggtag aactcccgac gctgaacctc
    12961 gctgtgcgct tcgccgtcca acagcatttc aacataagcc cctatgctgg aaagcggggt
    13021 ccgcagttcg tgcgataccg tcgagacgaa atccgttctg agttggtcga tctctcgctc
    13081 tcgggtgacg tcgtggaaca cggccacctg tccttgagcc gtaccggagt cgtcaatcac
    13141 tgtggagatc gtcatcttgt acgttcgggt agattcgccc atttggatct tgcactcggt
    13201 ctgccgatac cgtcgacact caccaagggc gcgggtgtcc gcgagaaggg cggccaggtg
    13261 cgcgtccttt acgagatcca cgatagcctg tccccgggcg tcggcaggct gaaacccgaa
    13321 cagcctgccg gctgccgcat tggccacggt caaccggcat tgagaatcca ccgccaacac
    13381 cggttcaccc agactgtgga tgatcgcctc gatcccgcgt ttggcagcct caacaccccg
    13441 catttgctcg tcgagagcgc gtttctgatc gcagatttgg cgcatgcgcc agtagactag
    13501 ggcaacgtat tcattaagtg cccccatgac cggcttgaga acgtccggca cgcatgtgtc
    13561 cagctcctcg atcccggggt ccccgcacat caaacggcga agtcggatct cgacgtgctc
    13621 cagactgcgc cgggcgatgc aacggcccag cagcaaaagg gcaacgacgg acatgaccag
    13681 cagggtgcaa accttccagg ctgccgcccg gtccggcggt gagacctgct ccgtgagacc
    13741 cgataactcg atgaccgcca cgagtattac tgccaccagc aggaccaggg tcagccacga
    13801 tgtccgtttg aatgagaaca gcgggcttct ggggtcacgg cgttcggcga tcacggggat
    13861 agacggtttc gtcgcctcga cgcacgcact catgatttct tcacgtccct cctgagcagc
    13921 gcaggctgaa cccgttgccg caggtcatgc tcggccgctc tacataatgt cggtcgggcg
    13981 ggagttcggc ttgatggcgt cggaggatta gcccaagttg aacactttcg aggcaagaat
    14041 ggccttggcg atagcgccag gg
//
LOCUS       DAOTPW010000148        14041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4257_length_14041_cov_5.226584, whole genome shotgun sequence.
ACCESSION   DAOTPW010000148 DAOTPW010000000
VERSION     DAOTPW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4257_length_14041_cov_5.226584"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /locus_tag="P5316_13775"
     CDS             complement(<1..1826)
                     /locus_tag="P5316_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HRR86073.1"
                     /translation="MLTHVSSLMLACWTVGSPSVISIEAPQTIGQYEVAEFVIRNDEP
                     VSANPFTEIDVTGTFGCPGGRHVVRGFADSQDGSVLRLRFCPDQAPTLYDYTINVAWP
                     GGGRRFTGRLQCEPSNRPGPVVVDPQHPRHFIRAGSREPFYHSGYTAYHLLDPSHTDG
                     QIDELIKYCADEGFNKIRFLLVGYPRDTGRQPAQTQGEYGVPDPFRAPNYGAPPGSVN
                     ALPAWLGEPHRYDFARFHVAYWQRVDRAIRNMREAGILATCVVTIEKQGLPREYGALT
                     EAEYRLYRYAVARLAAFDNVWWDLGNEHNEYRDPHWAETMGRFVKQEDPYDRLVSAHA
                     YADFPYAESSWADFIITQQYGDEREVHDWVLKYANAAKPYINEEYGYEGQGVRSQKGK
                     PDAPGHGQSHGWVRRCAWSIAMAGGYSTYGDWSRGISWFYMGRPGPGLAARQLKHLRR
                     FFEGLPFNRMTVHDELTTQGFCLALPSQHYVFYLPRGGEITIDLSAVRDMRLTSRWFD
                     PRTGQWREGPALQGGRSKLIAPDSDDWVLLVQREIPASTAVSAAVSPTWVSGNRYRIP
                     LSVDPRGVVRSCSPACADIDFSQALRDLQAGGSLDEESIEIVG"
     gene            complement(1973..4993)
                     /locus_tag="P5316_13780"
     CDS             complement(1973..4993)
                     /locus_tag="P5316_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86074.1"
                     /translation="MKRDTIAWALGLAILIDLCLLPVLSAAQSCEDHGTRASNTDPVS
                     SAQIHKHREGVIVPLGPTGSATPLTSINVTAEATFFSCMVRNFIVNVRAEFVLGPPDN
                     ESNNAFAVAFPITTHALYGDHNRFRDITVTVDGTPVSARAAMDYRLWKLVQNEVWYQG
                     RLVNHAIHPLLADAKDLNIVAPHLVARGYDLVIYWHVPAASISNTSRMVVTYTSNTDD
                     FSSCCHEVRDPALSSDPHHFYFDLSNARYWRGPKSCTVILKDSATALWSSVPVTNMDT
                     TFTEDSIILTRDPAGPDAELEDLAVPAQPAVFGSFPRRWPMPPPPVQTFTLSWRGGTS
                     GFIDRAVVDRIEPVGGIRVNAKLPMTLSKEHPSETFLASFMPPIADLAYIDVFGCGYS
                     GLYANGLYSAWLRIPMKGKCITGHDLAVKNLRVIPPGSSSEGWAVRATVANVGVFTEP
                     LLVTVLVNNRFVKTEGFRSLLPGDVVDLTIGVDWAWELPAEVEVEVEPAPGEVNRMNN
                     AAATCFPAPPLPRHANFVLQPDESNVLLRLDSGLTVRSKLLGAIKLYLGDPSVPVPFI
                     PGVIGLSVDGADLIAPFFSPTSSTGDVPVPLYMFQSPKVRSTGHWNTITGEIRFEMYL
                     VTPHGPAPAQQPTICSGRLVNTGLSVTGLSSEPTAIPKMILDIYAYEGPWPPQPDVWF
                     STEVGFHAGRLDPNLDNTIRYISAGDLLSRRGHVVRTNRELTQHLGIMPAVPDLGLDA
                     VVVGPRREIWFSFEEQLSPIWSETLAVWLKHGDLLSDRGYVVATNEQLVRRFGPMPVS
                     GDFGLDAAVRTPTRDLLFSTETDFFSETLGARVGHGDVLSSRGRIVRTNRQLLASFNP
                     VGPVVRDLGLDALVLRPFGEMWFSTEEGFIDANLGPISDGDLLSSAGYVVARNLDLLK
                     EFGPIEDLANFGLDAAGAIVPWSTSDFDQDGDVDESDLDNLTRAASGPGIPSPNPEAG
                     DFDGDGDVDQVDFGIWQRCYSGPDRLSDPECGAD"
     gene            complement(5393..6793)
                     /locus_tag="P5316_13785"
     CDS             complement(5393..6793)
                     /locus_tag="P5316_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR86075.1"
                     /translation="MADILIVDDEENLCYSVQLALHRAGHQCRVSDTMAGALRLCEDR
                     QPDLVLVDVQLPDGNGLDLIHRLREREVDAPVVVITAFGTVSTAVQAMKHGAVDFLQK
                     PLSMEELCLVVDRCLENRAIRTQLDAYRETEKRRFGEFRIVGECPQMKQVLATAKKIA
                     RLPGEPGRGLSTILILGETGTGKELVARYIHLHSLRPEQPFVHLNCTAIPEKLAESEL
                     FGHERGAYTDAKGAKKGLLEVADKGTLFLDEVGHMPLSSQAKLLVAIETGRFRRLGGL
                     NERVVDARIIAATNTDLESRARAGEFRLDLFYRLNVFCIRLPPLRERGDDVLVLADYF
                     LERFSRKFHKDPIRLSGETREILKRAHWPGNVRELANVLQQAVLLNESGIIEPSALNT
                     RVSDEISPSASRELCFDFKRDDCTLASVEKRLLQAAIAHTNGNISEAARLLGLTRGGL
                     RYRLEKLGLEPGPSAG"
     gene            complement(6794..8197)
                     /locus_tag="P5316_13790"
     CDS             complement(6794..8197)
                     /locus_tag="P5316_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR86076.1"
                     /translation="MQLRWKFGCLVLIYLLSLTANLVMSSWCIVVYFDSAFRRYETGL
                     DSEQSIQRVRALLRQHRETAQDNGRGVDFAAEGQTIQRELAATISRLRAADDLPSLAA
                     IAAVLEESSAGRPSSVSGPAGRSDLDVEQLDQLLGRAAMELEGRRQQSVLKAAATQWR
                     VMQIILINAVCGVLLCLVGLIFVRRWVIRPVAALRTAARHLAVGDFSHRIEVRSRDEL
                     GLLACEVNQMASRIAEMQAKLVEQERLAGAAEMVERLAHNIRNPLAGIRGLAEATSAR
                     HVDDAETVECQRRITDTVDRFEKWLRDLQASVSPMTLHPQPADVGELIRQAITVLQPM
                     LQRREVAIQVDVPTTMPPVHVDSMHFEQALVALLTNAVQASRPGQTVRVKAGPCPHRR
                     NSWQLVVQDDGEGISSEVRDKIFAPYFTTKRGGTGIGLTIAGRVAKAHGGEIVVDSTP
                     GHGSRFVVILPDQTAEA"
     gene            complement(8303..9058)
                     /locus_tag="P5316_13795"
     CDS             complement(8303..9058)
                     /locus_tag="P5316_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86077.1"
                     /translation="MRRRALTLMELVVVASITLVLASIVLVSLAQVRSTTRGFICKNN
                     LKTVAFEFAQFADGTTSPNRGESNRFGRRGFRIEDFQEKLYHIDEFWKGPKTGETEYL
                     ASDGPLICPTVQGKLRRMYGLACSEYAVGPSRNVSIGFNMRLDRASRQILSRWVLEPV
                     LLTDRILQHISVPIAFDVDGSLADDLGTPPYYSAPPAGDPGKYGSGRYWFPGTRHNQK
                     MNAVFVGGHVLSSGRPENEPGWNWRYQPPPSGY"
     gene            complement(9110..10915)
                     /locus_tag="P5316_13800"
     CDS             complement(9110..10915)
                     /locus_tag="P5316_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86078.1"
                     /translation="MRQGKDQSGAAAGGGLRSPRGRNAQPLAAKDNPRETQSRHFLQA
                     CNYPRAHYQAPGTYARRSGRFQIIASWAAVYLGILAGGAADEPVGLIEDRVGDAVVRR
                     MDMGADGPVDPDHHRQPDIIAVRISCWQPDVPHEDLFTGAVTTSGDFFRLDIIFDGLV
                     NPPGPTGCCGFPFAPFQYGNHPVFGYIEIDMDASRNKNTGGELDLPEWMYLGNAARFG
                     GLPVDSILASRCARDASAFDSNIMTPPLVDRSGEDFHIALHGWEITSVQGRVSADLVF
                     GPGDVWLVSGRLFPRARGYERFSFADTGGHPGRYNPIVHLRFAHSTSEDRTTVSLVYP
                     LNNRGSAAQMNAGVVEPLDGDAANQNSVLEALDDLVFSAANAPPIWRDDPAFALIAPW
                     ALLEPVPESNSAARFLDPLRWRITVLVGGSYTVPSNDVQFVWSDVLPDVRPDDFNGDG
                     RIDWRDRWLLERFILEHDGAAEKDKDGRINCVVSLTDFGTNFSVFDVNYDGRVDVADL
                     LAPPQFGRVPGDLDGDADVDQTDFGMLQACLGTSVSAPCLDADMDLDHDVDSDDGAAQ
                     EACASGPGIRADPGCWPPAILSCPATGLTAVGRSE"
     gene            11167..13170
                     /locus_tag="P5316_13805"
     CDS             11167..13170
                     /locus_tag="P5316_13805"
                     /inference="COORDINATES: protein motif:HMM:NF016100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86079.1"
                     /translation="MDNDPIEQLRLVPQRLDEVWQGGLIRMHSWVTGEGPKPYHPWAA
                     IWVAVKADKVGQPILLRPEEVDFSRAVDALVRFADDPCLGGYRPGRVQVRDKALAEYL
                     QELLAEVGIEVECTDHLPVFDRVVDALGGAMTGGPSPPDALSGQGVTLEKMRAFADAA
                     KAFYEAAPWNHLTDEDLIRIESPKPPPGMQVAAVLGASGRTFGLGFFSKPEELWAMHR
                     VDDPGEWFAARKRGAWCLTFDGITRLPLGDADLWEDHNLPVAGDSAYPCALCHDPSGE
                     VIRPNASVLAFLEGLLRALAGSTEAQIDSGRWTLTVETAAGPAEFTLSLPDLLKPPSH
                     KELMDRGFTPDRRALEQMYAQMDRFLANKHFENVDEVNAAISREFVGKPMDPSQFPPR
                     NALEKAQDLCYQAFDSIGRRRLQLARQALETCPDCADAYVLLAERTGDVNKAAELYAQ
                     GLAAGERALGPERFENDAGHFWGHTDTRPYMRARLGLAGCLERLSRNEEALDHYRELL
                     RLNPRDNQGVRYLYLPLLMKMGLDAEAARFMKNSQDEPTANWTYTRALLAYRLGGDCS
                     SARMELRKAVKVNPYVAGYLLADEDPDGIPESYSLGSREEAVVCASELRSAFAATPGA
                     LQWLKTQAGLMQRGGQSHHSSRGRRDRDLRRKRRSQERKRKRR"
     gene            13464..14027
                     /locus_tag="P5316_13810"
     CDS             13464..14027
                     /locus_tag="P5316_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86080.1"
                     /translation="MSEPLMDPRELQMPPEVSSTQASESGEAIPAETVPARDFSPEAE
                     AGKARGDEHLAAGAVAGASEVSAAQPEVSPFTAHQGRVARVTTEEVLILLEDGRQGTV
                     PLIEFAGQPLPKDGDGVSVIIEREDPQTGRVVLSKRHADELTFWESVQPGDELEGVVT
                     GMNKGGLDIDIGGAGHFFRPPRSTHTG"
BASE COUNT         2701 a         4358 c         4389 g         2593 t
ORIGIN      
        1 ccgacgattt caatgctttc ctcgtcgagc gagccgccgg cctgcagatc tcgcagtgcc
       61 tgggaaaagt cgatgtcggc gcaagccggc gagcaggacc ggacgacgcc tcgcgggtcg
      121 actgaaagag gaatgcggta ccggtttccg gacacccagg tcggactgac ggccgcagat
      181 accgcagtgc tcgcagggat ctccctctgg acgagcagga cccaatcatc actgtctggt
      241 gcgatcagct tagaccggcc gccctgcaag gcggggcctt cccgccattg tcccgtacgt
      301 gggtcgaacc atcgcgaagt cagccgcatg tcccgaaccg cggacagatc tatggttatt
      361 tcgccgccgc gaggcaggta gaacacatag tgctgcgagg gcagcgccag acagaatccc
      421 tgtgtcgtca gctcgtcgtg gacagtcatg cggttgaagg gcagaccctc aaaaaaacgg
      481 cgaaggtgtt tgagttggcg cgcggccagg cccggtcccg gccgacccat gtagaaccaa
      541 cttattcctc ggctccaatc gccgtatgtc gagtagccgc cggccatggc gatggaccac
      601 gcacagcgtc ggacccaacc gtggctctgg ccgtggccgg gggcatcggg tttgcctttc
      661 tggctgcgga cgccttggcc ctcgtagcca tactcctcat tgatgtaggg tttcgccgcg
      721 tttgcgtatt tgaggaccca gtcgtgaacc tcgcgctcgt caccgtattg ctgggtgatg
      781 atgaagtcgg cccaactcga ttcggcataa gggaaatcgg cgtaggcgtg agcagaaacg
      841 agccggtcgt atgggtcttc ctgcttgaca aagcggccca tggtctcggc ccagtgcggg
      901 tcgcgatact cgttgtgctc gttgccgagg tcccaccaca cgttgtcgaa agccgccagg
      961 cgagcgaccg catagcggta caaccgatac tctgcttccg tcaatgctcc gtactcgcgc
     1021 ggcagaccct gcttttcgat cgtcacgacg cacgttgcca ggatgcccgc ctcgcgcatg
     1081 ttcctgatcg cacgatcgac acgttgccaa taggcgacat gaaagcgggc gaaatcgtag
     1141 cgatggggct cgccgagcca tgccggcagg gcgttgaccg aacctggggg cgcaccatag
     1201 tttggcgccc tgaacgggtc gggcacgccg tattcgcctt gcgtctgagc cggttgacgg
     1261 cctgtgtctc gcggataacc gaccaggagg aaacgaatct tgttgaagcc ttcgtccgcg
     1321 cagtacttga tcaactcgtc aatctgtccg tcggtatggc tggggtcgag caaatgatag
     1381 gcggtgtagc ccgaatggta aaacggttcg cgagaaccgg cgcgaatgaa gtgacgagga
     1441 tgctgagggt caacgaccac cggtcccggt cggtttgaag gctcgcattg cagccggcct
     1501 gtgaaccggc gaccaccgcc cggccaggcg acgttgatgg tgtagtcgta tagagtcggc
     1561 gcttgatcgg gacagaatcg taatcgcagg acactgccgt cctggctgtc ggcaaagccc
     1621 cgaaccacgt gtctgccgcc cggacacccg aacgtgccgg tcacatcaat ctcggtgaaa
     1681 ggattcgcgc tgacaggttc gtcgttgcgg atgacgaact cggccacctc gtattgccca
     1741 atggtctgcg gagcctcaat ggagatgaca gagggggatc cgacggtcca gcacgccaac
     1801 atgagtgagg agacgtgggt cagcatgacc gcattgtaca agcggcatca ggaaagcaaa
     1861 ggtacagggg cgaccgccgg acggaattga aagccccgcg cctgcgcagg gacacgaaca
     1921 ggaccggatg tccagacttc accggctgct gtgactgtgc ttgacttccc gatcagtcgg
     1981 cgccgcactc cgggtcggaa agacggtcgg ggcccgagta gcatcgttgc cagattccga
     2041 agtcgacctg atcgacgtcg ccgtctccat cgaagtcgcc ggcctcggga ttcgggctgg
     2101 ggatgccggg tccgctggcg gcccgggtca agttatccag gtccgactca tcaacgtcgc
     2161 catcctggtc gaaatcggaa gtactccagg gcacgatcgc gccggcggcg tccaggccga
     2221 agttggccag gtcttcaatc gggccaaact ccttgagcag gtccaggttc ctggccacca
     2281 cgtatcccgc tgacgagagc agatctccgt cggagatcgg gccgaggttg gcgtcgatga
     2341 acccctcctc agtggagaac cacatttccc cgaatggccg gagcacaagg gcatcgagac
     2401 caagatcgcg gaccaccggg ccaaccggat tgaaactggc caacaactgc cgattggtgc
     2461 gtacgatccg ccctcggctg gacagaacgt cgccgtgccc gaccctcgcg ccgagggtct
     2521 cggagaagaa gtccgtttcc gtggagaaca gcaggtcccg agtcggcgtt cggacggccg
     2581 catcgagccc aaagtcgcct gataccggca tgggcccgaa ccttcgaacg agttgctcgt
     2641 tggtggccac gacgtagcca cgatcggaaa gcaggtcgcc gtgtttcagc cagacggcaa
     2701 gtgtctcgct ccagatgggg ctcagttgct cctcgaagga gaaccagatt tcgcgccgag
     2761 gcccgacgac aaccgcatcc aacccgaggt ccggaacggc cggcatgatt ccgagatgct
     2821 gagtgagctc ccggtttgtt cggacgacgt gcccccgccg gctcagcaga tcgcccgcgc
     2881 tgatgtaccg gatggtgtta tccaggttgg ggtccagacg gccggcatgg aatcccacct
     2941 cggtggagaa ccagacgtcc ggctgcggcg gccaaggccc ctcgtaggca tagatgtcca
     3001 ggatcatctt ggggatggcc gtcggttcgg agctcagacc ggtgacactc agcccggtat
     3061 tgaccagcct tcccgagcag atcgtgggtt gttgagccgg cgccgggccg tgtggcgtca
     3121 cgaggtacat ttcgaaccgg atttctcccg taatcgtgtt ccagtggccg gtcgaacgta
     3181 cctttggcga ctggaacata tacagcggca cgggcacgtc gccggtcgat gacgtcggcg
     3241 agaagaacgg cgcgatcagg tcggcgccgt ccaccgacag gccgatcaca ccgggtatga
     3301 acggtacagg cacgctggga tccccaaggt acagcttgat cgctcccagc agcttcgatc
     3361 gaacggtcag gcccgaatcg agccggagca gaacgttgct ctcatcgggc tggaggacga
     3421 aattggcgtg ccggggcaga ggaggcgccg gaaaacacgt cgccgctgcg ttgttcatgc
     3481 ggttcacctc gccgggtgcg ggctccactt caacttccac ctcggcgggc agttcccacg
     3541 cccagtccac gccgatcgtc aagtcaacga cgtcgcccgg cagtagacta cggaaaccct
     3601 ccgtcttgac gaaacggttg ttcaccagaa ccgttaccag gagaggctcg gtgaacaccc
     3661 cgacgttggc caccgtcgcg cggacggccc agccttcgga ggaactgccg ggcggaatga
     3721 ccctgaggtt cttgaccgcc agatcgtgac cggtgatgca tttgcccttc attgggatgc
     3781 gcagccacgc cgaatagagg ccattggcgt acagacccga gtagccgcag ccgaatacgt
     3841 cgatgtacgc cagatccgcg atcggcggca tgaaggacgc cagaaacgtc tcagagggat
     3901 gttccttgct caaagtcata ggcagcttgg cgttcaccct gatgccgccc accggttcga
     3961 tccgatcaac gacagcacga tcaatgaagc cgctggttcc ccctcgccag gagagagtga
     4021 acgtctggac aggcggcggg ggcatcggcc atcgccgcgg aaacgagccg aataccgcag
     4081 gctgggcagg tacggcaagg tcttcgagtt ccgcgtctgg gcccgcggga tcgcgggtaa
     4141 ggatgatcga atcctcggtg aacgtcgtat ccatgttggt cacgggcacc gaagaccaca
     4201 atgcggtcgc cgagtccttc aagatcaccg tgcacgattt cgggccccgc cagtatctgg
     4261 cgttgctcag gtcgaagtag aagtggtggg gatccgaact caaggctggg tcacgaactt
     4321 cgtggcagca actggagaaa tcgtcggtgt tactcgtgta cgtgaccacc atgcggctgg
     4381 tgttgctgat cgatgccgcc ggaacgtgcc aatagatgac caggtcgtac ccgcgggcga
     4441 ccaagtgcgg agccacgatg ttcaaatcct tcgcatcggc gagcaggggg tggatggcgt
     4501 ggttgaccag ccgtccctgg taccagacct cgttctgcac gagcttccag agacggtagt
     4561 ccatcgccgc ccgagccgat accggcgtgc cgtcgacggt gacggtgatg tcacggaagc
     4621 ggttgtgatc gccgtacagc gcatgcgtcg tgatcgggaa ggccaccgcg aaggcgttgt
     4681 ttgactcgtt atcgggaggc ccgagaacga actccgcccg gacgttcacg atgaaattgc
     4741 ggaccatgca actgaagaag gttgcctcgg ccgtgacgtt gatcgaggtt aacggcgtgg
     4801 cgcttcccgt cggtcccaag ggcacgatca cgccttcccg gtgcttgtga atctgcgcag
     4861 aggaaaccgg atccgtgttg cttgcccgcg tcccgtggtc ctcgcagctc tgtgccgcgg
     4921 acaacaccgg caagagacac aagtcgataa ggatcgccag acccagcgcc catgctatcg
     4981 tatctcgctt catcgttgac ctccgcttcc agtggatgag gttgcccaca cttcgcagaa
     5041 ccacagatac cggtctcggg gcccggattt cctcggggcg gcctgtagtt gttcagccga
     5101 caaaacggta ccggctcgcc aatgcagggg ccctgcgccg cccgttcttg ggaacaccga
     5161 cagcagatta cgtaccgctg cctgaagaat tgcccgatga acgatataag ccgatgaccg
     5221 ataacacttt gcgcgagccg gcccgttttc cggggcaggg tacctgcggt ggctgtcctc
     5281 agccaactgg ccgagcattc agccggaagc gagccagcag gcgaagcggc gtccggccga
     5341 ggtgtggcgc atcgcccgca gcacggatta gattgctcac cgcggtcggg tgttaccctg
     5401 ccgacggacc cggttcgagg ccgagcttct cgaggcggta gcgtaggccc cctcgggtca
     5461 ggcccagcag gcgggctgcc tcggagatat tgccgttcgt atgggcgatg gccgcctgca
     5521 acaatctctt ctcgaccgat gcgagcgtgc agtcgtcccg cttgaagtcg aagcacaact
     5581 ctctcgaagc acttggggag atctcgtcac tcacacgcgt gttgagggcc gagggctcga
     5641 tgatccccga ctcgttgagc agcacggcct gctgaaggac gttggccagc tcgcgcacgt
     5701 tccccggcca gtgagcgcgt ttaagtattt cccgggtctc gccggacaag cggatcggat
     5761 ccttgtggaa cttgcgggag aagcgctcca ggaaatagtc ggccagaaca agcacgtcgt
     5821 cgcctcgttc tcgcagaggc ggtaacctga tgcaaaagac gttgagccgg tagaagagat
     5881 ccagtcggaa ctcaccggcc cgggcccggc tttccagatc ggtgttggtg gccgcgatga
     5941 tacgggcatc gacgacccgc tcgttgagac ctcccagtcg ccggaatcgg ccggtttcaa
     6001 tggccacgag cagcttggct tggctggaca gcggcatgtg gccgacctca tccagaaaga
     6061 gtgttccttt gtcagcgact tcgagaagac ctttcttcgc gcctttggcg tcggtgtagg
     6121 cgccgcgctc gtggccgaaa agctccgact cggcaagctt ttcgggaatc gcggtgcagt
     6181 tgagatgcac gaacggctgt tcaggccgca agctgtgcag atgaatgtaa cgggcgacga
     6241 gttccttgcc cgtgccggtc tcgcccagaa tcaggatggt cgacaggcct cttccgggct
     6301 ctccaggcag ccgtgcgatt ttcttcgccg tcgccagcac ctgtttcatt tgtgggcatt
     6361 ccccgacgat acggaattcg ccgaagcggc gtttctcggt ctcgcgatag gcatcgagct
     6421 gggtgcggat cgcccgattc tcaaggcagc gatccacgac gaggcatagt tcttccatgg
     6481 acaacggttt ctggaggaaa tctacagccc cgtgcttcat cgcctgaacg gccgtggaca
     6541 cggtcccaaa cgccgtgatg actaccacgg gcgcatcgac ttcgcgctcc cgcaaccggt
     6601 gaatcagatc cagcccgttg ccgtcgggaa gctggacgtc caccaggaca aggtcgggct
     6661 ggcgatcttc gcacaagcgc agcgcgccgg ccatcgtgtc ggagacgcgg cattgatgtc
     6721 cggcgcgatg gagagcaagc tgcaccgaat agcacaggtt ttcctcgtca tcgacgatca
     6781 ggatgtccgc cattcaagcc tccgctgtct gatccggtaa aatgaccacg aagcggctgc
     6841 catgcccggg cgtcgagtcg accacgatct cgcccccgtg ggccttggcg acgcggccgg
     6901 caatggtcag gccgattccc gtgcctcccc gcttagtggt gaaatacggg gcaaagatct
     6961 tgtcacgaac ctcggaggat attccttctc cgtcgtcctg cacgaccagc tgccagctat
     7021 tgcggcggtg ggggcagggg ccggccttta cccgcaccgt ctgacccggc cgggaggcct
     7081 gcacggcgtt cgtcagcagg gccaccagcg cctgttcgaa atgcatgctg tctacatgca
     7141 cgggcggcat tgtggtcggg acgtcaacct ggatcgcaac ctctctccgt tggagcatgg
     7201 gctggagcac cgtaattgcc tgcctgatca gttcgccgac gtccgcgggt tgagggtgca
     7261 gcgtcatcgg cgataccgac gcctgcaggt cgcgaagcca cttctcaaac cggtccacgg
     7321 tatccgtgat ccggcgctgg cactccacgg tctcggcgtc gtcgacgtga cgagcgctcg
     7381 tcgcctcggc tagaccgcgg atgccggcca gggggttgcg gatgttgtgg gccagacgct
     7441 cgaccatctc ggcggccccg gccagccgct cctgctcgac gagcttggct tgcatctccg
     7501 caattctcga agccatctgg ttcacctcgc aggccagcag acccagctcg tctcgagatc
     7561 tgacttcgat acgatgactg aaatctccga cggccaggtg ccgggctgcc gtccggagag
     7621 cggccaccgg ccggatcacc catcgccgga cgaagatcag gccgaccagg cagagcagga
     7681 ctccgcacac cgcgttaatc agaatgatct gcatcacccg ccattgtgtt gccgccgctt
     7741 tgagcacgct ctgctgtctc cggccctcca attccatggc cgcccgtccc agcagttggt
     7801 ccagttgctc cacgtcgagg tctgaccgac cggccggccc gcttaccgaa gacgggcgac
     7861 ccgcgcttga ctcctccagt actgctgcga tcgcggccag tgagggcaag tcgtctgccg
     7921 cacgcagtct cgagatcgtc gccgccaact cacgctggat ggtctgccct tccgcggcaa
     7981 agtccacgcc ccgcccatta tcttgggcgg tttcccgatg ttgccgcagc agggcgcgga
     8041 cacgttggat gctctgttca ctgtccaagc cggtttcgta ccggcggaag gccgaatcga
     8101 agtacaccac gatgcaccaa ctggacatga cgagattggc cgtcagcgac agaagataga
     8161 tcaggacaag gcagccaaac ttccaacgga gttgcacgac aatacctgac cgtgagagcc
     8221 atgcgccttt tgcgcgcgtc tttcgtaccc acacgatgac ctggtagcgg cccgcaaagc
     8281 agccgttgat ccagagtctt actcaataac cgctcggtgg aggctggtac cgccaattcc
     8341 agccgggctc gttctccggc ctgccggacg agaggacgtg gccgcccacg aaaaccgcgt
     8401 tcatcttctg attgtggcgc gtcccgggaa accagtaccg gccgctgccg tacttgccgg
     8461 gatcgcccgc cggcggcgcg gaataatagg gcggcgtacc aaggtcatcg gccagggacc
     8521 cgtccacgtc aaaggcgatc ggtaccgaga tatgctgcaa aatgcgatcc gtcagcagca
     8581 ccggctccag cacccatcgc gacagaatct gcctgcttgc ccggtcgagc cgcatgttga
     8641 atccgatgct cacgttcctg gacgggccaa ccgcatactc ggaacaggcc aggccgtaca
     8701 tgcggcgcag ctttccctgg accgtcgggc agatcagagg tccgtccgac gccaggtatt
     8761 cagtctcgcc tgtcttcggt cccttccaga actcatcgat gtgatagagt ttctcctgaa
     8821 aatcctcgat ccggaacccg cgtcgcccga accggttgct ctcaccgcgg tttggggacg
     8881 tcgtgccgtc ggcaaactga gcgaactcga acgccaccgt tttgaggttg tttttgcaga
     8941 tgaagcctct ggtcgtggaa cgtacctggg ccaacgacac gagcacgata ctcgccagca
     9001 cgagggtgat ggatgcaacc acaacaagtt ccatcagggt gagggccctt cttctcatcc
     9061 tggttggatc tcgcgcgccg ttcactggtt tctttccctt atagcatccc tactccgacc
     9121 gcccaaccgc agtcaatccg gttgccggac acgacaggat tgcaggcggc cagcagcccg
     9181 gatcagcccg aattcccggc ccgctggcgc acgcctcctg cgcggcccca tcgtcactgt
     9241 caacgtcatg gtcaagatcc atgtcggcgt ccaggcacgg cgctgagact gacgttccca
     9301 ggcacgcctg gagcatgccg aaatccgtct ggtccacgtc cgcgtcgcca tcgagatcac
     9361 ccggcactcg gccgaactgg ggcggtgcga ggaggtcggc aacgtccacc cggccgtcat
     9421 agttcacatc gaaaacgctg aagttcgtcc cgaaatccgt gagactgacg acacaattga
     9481 tccgcccgtc tttgtccttt tcggctgcgc cgtcgtgctc caggatgaaa cgttccagaa
     9541 gccatcgatc tcgccagtcg atccggccat ctccgttgaa atcgtcgggg cgtacgtcgg
     9601 ggaggacgtc gctccagacg aattggacgt cgttgctggg gaccgtgtag ctgccaccga
     9661 cgaggaccgt tattctccat cgaagcgggt caaggaatcg agccgcgcta ttgctctcgg
     9721 gcaccggttc gagcaacgcc catggagcga tgagagcaaa tgccgggtca tcccgccaga
     9781 tgggtggggc gttggcggca ctgaacacca ggtcatcaag tgcttccaga acgctgttct
     9841 ggttggcggc atcaccatcc agaggctcaa ccacgccggc gttcatctgc gcggccgagc
     9901 ctcggttatt gagcggatat accagggaga ccgtggtccg gtcctcgctt gtgctgtgcg
     9961 caaagcgcag atgcacgatg gggttgtacc tgcccggatg gccgccggtg tcagcgaagc
    10021 tgaagcgttc gtagccgcga gcccggggaa acagccgacc actgaccagc cacacatctc
    10081 ccgggccgaa cacgagatcc gccgacaccc gtccctggac gctggtgatc tcccagccgt
    10141 gcagcgcgat gtggaaatcc tctccgcttc gatcgaccaa cgggggggtc atgatgttgg
    10201 agtcaaaggc cgaggcgtcc cgagcgcaac ggctcgcgag gatggaatca accggcaaac
    10261 cgccgaaccg cgctgcgttg cccaggtaca tccattccgg cagatctagc tcacctccgg
    10321 tattcttgtt tctggaggcg tccatgtcga tttcaatgta gccgaacacc ggatgattgc
    10381 cgtactggaa gggcgcgaat gggaaaccgc aacagccggt cgggccgggc gggttgacca
    10441 ggccatcgaa gataatgtcg aggcggaaga aatcgcccga tgtcgtgact gcgcccgtga
    10501 agagatcctc atgaggcacg tcaggctgcc agcaggagat gcgcacggcg atgatgtcgg
    10561 gttgacgatg gtgatcagga tcaaccggcc cgtccgcacc catatccatc cgacgcacca
    10621 ccgcgtctcc cacccgatcc tcaatcaagc cgacgggttc gtcggccgcg ccgccggcca
    10681 gaatgcccag gtaaacagcc gcccacgagg cgatgatttg gaatcgcccc gatcgtcttg
    10741 cgtatgttcc cggggcttgg taatgcgctc ttgggtaatt gcaggcttgc aggaaatgcc
    10801 tgctctgggt ctcgcgcggg ttgtcctttg ccgccaacgg ttgagcattc ctgccgcgcg
    10861 ggcttcgcag accgccgccg gcggcggcgc cagactggtc tttaccctgc ctcatgtgcg
    10921 gtccctgctg ccgccagatg gatatgcgtc ttgtcggcct ctattatacg cgggagcagt
    10981 tgagacccca aaccttttcc gccaagatgt atgcgaatag gagtcggcgt tcgcgactca
    11041 gaggcggccc ggcaaagtct caacgcggcc aagccgcgca ggggtgaggt tggccgcctc
    11101 tgcagcgaaa ggttggcctc ccgcgtccgt gttgacccga ccccgtttct gtgggaagat
    11161 tttaccatgg acaacgatcc aatcgaacaa ctcagactgg tcccgcagcg tttggacgag
    11221 gtctggcaag gcggcctgat tcgcatgcac tcatgggtta cgggagaagg cccaaaaccg
    11281 taccacccgt gggccgccat atgggttgcg gtcaaagctg acaaggtcgg tcagccgatt
    11341 ctcctgcggc cagaggaggt cgatttctcg cgggcagtgg acgctctcgt gaggtttgcc
    11401 gatgacccct gtcttggcgg ctatcggccc ggcagggtgc aagttcgcga caaggcattg
    11461 gctgagtacc tgcaagaact gctcgccgag gtcgggattg aggttgagtg cacagaccat
    11521 ctgccggtat tcgatcgggt tgtggatgcg ctgggcgggg cgatgacggg tggaccttcg
    11581 ccgccggatg ccctttccgg ccaaggcgtg accctggaga agatgcgtgc cttcgccgac
    11641 gcagccaagg ccttctacga ggcagccccc tggaaccatc tgactgacga agacctgatc
    11701 cgcattgaat cacccaagcc gccccctgga atgcaggtcg cagcagtgct gggagcttcc
    11761 ggccgcacct tcgggctggg tttcttctcg aaaccagaag agctttgggc gatgcatcgt
    11821 gtcgacgatc caggtgaatg gtttgccgcc cggaagcgcg gcgcatggtg cctcaccttt
    11881 gatggcatca ccagactccc attgggtgac gcggatctct gggaggatca caatctgccg
    11941 gtagccggcg attccgccta cccctgtgcc ctgtgccatg acccgagcgg cgaggttatc
    12001 cgcccgaatg cctccgtcct agccttcctg gaaggtctgc tgcgggcact ggcagggagc
    12061 actgaggcgc aaatcgattc cggccgatgg accctgacag tggagaccgc cgccggcccg
    12121 gccgagttca ccctgtcgct gcccgacctg ctcaagccgc caagccacaa ggagctgatg
    12181 gaccggggct tcacgccaga tcgccgggcg ctagagcaga tgtacgccca gatggacaga
    12241 ttcctagcca acaagcactt cgagaatgtc gacgaggtca acgcggccat ctcgcgcgag
    12301 tttgtcggca aacccatgga tcccagccag tttccccctc gcaacgccct ggagaaagcc
    12361 caggatctgt gttaccaggc ctttgacagt atcgggcggc gccggcttca actggcccgc
    12421 caggcgctgg agacctgccc cgactgtgcc gacgcctatg tgttgctggc cgaacgaacg
    12481 ggcgatgtca acaaggctgc tgaattgtac gcgcaaggtc tggctgccgg cgagcgtgcg
    12541 ctgggaccgg agcgattcga aaacgacgcg ggccatttct ggggccacac agacacgcgg
    12601 ccgtacatgc gggcacgtct gggcttggcc ggctgcctgg aacggttgag ccgaaacgag
    12661 gaggcgctcg atcattaccg cgagctgctg cggctgaacc cccgcgacaa ccagggggtg
    12721 cggtatctct acctgccgct gctgatgaag atgggtctgg acgccgaagc tgctcgattc
    12781 atgaagaaca gccaagacga accgaccgcc aactggacgt acacccgggc cttgctggca
    12841 tatcggctgg gcggtgactg ctcgtccgct cgtatggaac ttaggaaggc agtcaaggtg
    12901 aatccctatg tggccggcta tctgctggcc gacgaggatc cggacgggat accggaatcc
    12961 tacagtttgg gcagccgtga agaagctgtt gtctgcgctt ctgagctccg atcagccttt
    13021 gcggccacgc cgggcgccct gcaatggctg aagacccaag ccggcctcat gcagcgcggg
    13081 ggtcaatcgc atcactcttc gcggggccgc cgtgaccgcg accttcgccg aaaacgccga
    13141 tcacaggaac gtaagcgaaa acggcggtga gcataggcaa caagtgtctg atccgaccgc
    13201 tctgaatacc atcagtcaag ccctattctt ttcaatgcgg aaacttgaaa cacccttcgg
    13261 tgcccaactt ggaccaaacc gttgtgccag gtcacgacgc accggcgaga gcaccgatac
    13321 cggcaggggt tgaccaacac ccagtggtcg gacaagatga tggctcaggt agttccgttc
    13381 atgttcctgg tagttgatcg aaactcacag gcttggtcgg gctgcccggg attgatatct
    13441 aggtgagcga gaagagacca cccatgagtg agcccctgat ggaccctcgg gagctgcaga
    13501 tgccgcccga ggtgtcctcc acccaggcat cagagagtgg cgaggctatt ccggccgaaa
    13561 cagtgccggc cagggacttc tcccccgagg cggaggcggg caaggctcgc ggagacgagc
    13621 atctcgcggc tggtgctgtc gcgggcgcat ccgaggtatc cgcggcccag ccggaagtat
    13681 cgcctttcac ggcacaccag gggcgagttg cccgtgtcac gaccgaagaa gtccttatcc
    13741 tgcttgagga tggacgtcag ggcacggtgc cattgatcga atttgccggc cagccgctgc
    13801 cgaaggatgg tgatggggtc tcggtgatca tcgagcggga agacccgcag accggccgag
    13861 tcgtcctcag caagcgtcac gccgacgagt tgaccttctg ggaatccgtc cagcccggtg
    13921 acgagcttga aggcgtggtc acgggcatga acaagggcgg cttggacatc gacatcggtg
    13981 gtgccgggca tttcttccgg cctcccaggt cgacacacac cggatgaaag acatctcgct
    14041 g
//
LOCUS       DAOTPW010000149        13995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4275_length_13995_cov_4.981277, whole genome shotgun sequence.
ACCESSION   DAOTPW010000149 DAOTPW010000000
VERSION     DAOTPW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4275_length_13995_cov_4.981277"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1417
                     /locus_tag="P5316_13815"
     CDS             86..1417
                     /locus_tag="P5316_13815"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86081.1"
                     /translation="MNAKAVWTLMKERLPAWRRAQDNGHQSSVLLPADEPSPPTKMRP
                     ATGIVDASLPDDQRWPAMMRAGTRHYESGEFRLAVDAFRAAAQLARGFGSDDPRLAVS
                     LNDWAEACRACGDWAQALSLCQESLALRGRVLGPSHPHVGVSLHNLGTLFLETGRLAE
                     AALAFERALTLLRGGLGCGHPDVAATLCGLAAVMKAEGHHPDAAEYVRQALAIRQEAF
                     GWRHPEVAACLSALGDIHRELGEYDKARNLCEQALAMTRELLGHEHFAVAAILNNLAL
                     LNKTQGRLAEAELLYRQALAIEERTLGPQHPSLAATLNNLAVLCLSLRRLTDAESLLR
                     RALSICRDKSPTNHPDVGLILANLAETCRLSNRLPEAESLCYQALSVAESALPPNHPH
                     RAAILNNLGVILCARGQRKQSKVLLENAAAVLEQRPADDRLAHVVRRNLRA"
     gene            complement(1593..2279)
                     /locus_tag="P5316_13820"
     CDS             complement(1593..2279)
                     /locus_tag="P5316_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86082.1"
                     /translation="MSAELLWEALRFRTQKQEGGAARIEPTQIYAREPKQKQCAATTR
                     SGRRCRGRARPGQDFCPFHDPNLTAEQRREIASRGGRSHRRLSHLPDGYLRKLTTPAA
                     VGEAMDRLYREVRLGIVDPAMGRTLMDILTRLHDRLSRQGASGPQVARKAPTVVQPTK
                     AERVRPKLDHALSQAEMAAWRRVGIMPQGISSDDESPSHLVHPVLSAPPEPRSETVKP
                     LVQIVRANAS"
     gene            2905..4797
                     /locus_tag="P5316_13825"
     CDS             2905..4797
                     /locus_tag="P5316_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86083.1"
                     /translation="MFASQSGSLSAQFTGLDWLVVIGYLALTTAIGSLMAGKQATIRD
                     FFLGGRKLPWYAVCGSNIATEISAVTFVGVPAIVFASQGNFTYLQLQLIGLVLARLIV
                     AYVFVPAYYEREIYSPYDYMANRLGEEARTVTTCLFILGGSLAQGARVYLTAMILDII
                     AGKSLYGPLADATGISTLTWSIYTFGIISVVWCWIGGITTVIWTDVILFLVFVVGAFV
                     ALGAAVVRLPGGWSELISIGWNAKQAGRWGKFTVFDFDLSLIKPYTFWAALIGYTWHG
                     LFAFGTDQIMAQRMFCCKGPRPARWAVIGSNLGQIIAIVMLMVGVGLYAFYQKNPLSG
                     EALDLVRKDINYVFPVFIIQELRPGITGLIIAAIFAAAISTLISVVASFSQTTLSAFY
                     LPWYRRRSTRKGSPVGDEQAMQNPRLVLLSRLLVLIWGAILCLTAQLAHAAAQHYKQI
                     LDLALAMAGYAGGALLAGFMLAFLRINVDARGFIWSAPLSVLTVLAVAWHQPWAHAIC
                     WAGTVILLATWLFHIYGRRKDMPAGKQAPEPVLSPAVQTVILAVGTAIMMVISYFGYF
                     ETVDAATGKMTRTTLAWPWFIAIGSLVAFSFGWLLAGRKDTAAARDTAGQQNDLSNNG
                     DTSGTG"
     gene            complement(4791..4863)
                     /locus_tag="P5316_13830"
     tRNA            complement(4791..4863)
                     /locus_tag="P5316_13830"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4828..4830),aa:Phe,seq:gaa)
     gene            5287..6408
                     /locus_tag="P5316_13835"
     CDS             5287..6408
                     /locus_tag="P5316_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRR86084.1"
                     /translation="MLPFAKKGPCRPTIRAIAEMAGVSRTTVSLILANRRDMIDRFRP
                     ETVDRVRKAAETLGYRANLLALSLRSPRPSFFALVLRGPGQVDPDSVSWHHQAFEGMF
                     LAGALEASRALRLYPVVATQDLPKTEDSLLALTEVLEGGVFGAVIRSPVAVLHEPLSR
                     HIQQGMPIVTVFPERPDLYPTNVIDLDNAATGELAGRLLHEAGRRRWLFLLQADVWEA
                     LRIRLAGLRRSAEQAGSSLEELVVPAHLAEADVPGWLAARLKDMKIDGVYAPSALSAV
                     RALYACTAGGLRVPEDVCVVGTDAALWRARGFPIITSIDVSWFRAGDLAVRRMAELRD
                     SGESFFENVCLPPIVHAGDSCPSNLSGPVTATAGEPQRV"
     gene            complement(6582..8621)
                     /locus_tag="P5316_13840"
     CDS             complement(6582..8621)
                     /locus_tag="P5316_13840"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRR86085.1"
                     /translation="MRRLVAMLPGLVGVVVSAAPAEAEELAPGVTYTVYNVAGPNMVH
                     VVAAERLHSEYKLKVGWAQGKRNYTAREGTSTIAGRYDNPPEHDVLSAVNGSYFDGVN
                     LPRLIGIGQSDGQMLDTPAFNSSYQYHTVMVGPSRRPVVRTNFNHVLGTLTFADGHSM
                     PLNQYNFYMNGPLVPINGVAAFTPHFDSSTRSSFITPSMAVEVVLNDVSYPMRSHKEV
                     SGIISAVHTPTEGNAAIPVGGMVLSAWGSTKSEIVSHAHVGDRLRMRFATDAEEFNDS
                     DNALTGIGWVIRNGAAYTPGWANLESGAAPYGRNPRTVLAWNDSYWFQVVCDGRSAAS
                     VGMTFQEMASFLVGTLGATEAVNYDGGGSSTMVVNGAIRNVPSDGSERPVANAIMLVK
                     QSTASVFPFTDSFGPAGRLAGWDDKFCYADVVSFSPTSPGGDGYVLKVMNRYGGADSV
                     RRGDFGDTDYTVEADVYCEYRPEAAADGYELSGIIARDSGTGAMGLSSFGKGNCYALL
                     YASDTGLIKAGKYVNGMWTDFAADVPGTPLTGWHRFRIDAYGDRIRYRLDDTAICTVR
                     DSSHPRGYYGVGYHEMFNTDANMRGARVDNFIAHVVPGAILVVDYDDDTDVDQVDFAH
                     LQLCLSGSQVAQILPDCQDARLDNDSDVDADDMAIFIGCLSGSGIPAEPDCMSNP"
     gene            complement(8703..9788)
                     /locus_tag="P5316_13845"
     CDS             complement(8703..9788)
                     /locus_tag="P5316_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR86086.1"
                     /translation="MKSPLRTGFTLIEVLVVVAIIALLIAILLPSLSRARKEVQAAVC
                     ASNMRQGIQGVVLRQAETQMRKEYWSTNFGWAVHSLKQNKGQTELFTCPSDPKPRPIP
                     AVQDRLYSDAAGTDYRGVTTGDAIFCRLARNGSTWTTDIEDQVDETLFGGDAYSDNDG
                     DFLIQYTVASRETFAPAKMWKPGSTAWRHDVMTYKGEIIAQNVSSSGVIKTVPILWMS
                     FSANASAGFKNARGTPVLIVEGAKFGIFAEALGSNYPSDNLARALRFRHGGLVKREGI
                     EGAEWIGNYTGGAWPAAPPQTGSLGNSRDLDYQPRERMNAGFLDAHVERMGWWEMFRM
                     HPTVPTNKPSPVIPLWFGSRKGDISFD"
     gene            complement(9966..12977)
                     /locus_tag="P5316_13850"
     CDS             complement(9966..12977)
                     /locus_tag="P5316_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86087.1"
                     /translation="MHTSCRIKNGFCGILVIAVFLGWTLAARAQFTAYNDFVPDYVAG
                     ATNVTQNQNAGTYALKDYATGATVTPTMTITRSGLSTDGTGPSAEISGGDALTIFGGK
                     VAGRGKVMAPSTGWYFTMEFSGLDVNKTYTVATFTDRGEPSYDNKRWANISLVNADST
                     IATYACSVGVGNYQVSPTSVSQDSGYNTVEGFVAMWTGIAPGPDGKFSVHFRAATPAE
                     VPEAYRAGMKADSGYGPAGLMLVEEGPPNAVNISPGVINTVVGGATVSGTVKIPADSN
                     ADAPVQVTLTSDNPAVADLVGATGGVLVLTFPQGGTTEQGIQIDIGQAGTATISSNND
                     AELVDDTLPVTVETGAVTLAPASLTGTPGTTRPVTVSISPGTNDTRTVEVTLTSDNPA
                     VADFTGGSGGVLVLSFPQGGATQQTANVRLGVLGEATITSTNDGGLTDPDGVSVSVSR
                     LRSWVLRIRELQGSGWHMVPTGGYDDGIHLGSYPLDGRYYWREYNADETWPRAYWRVE
                     RSDVPGGTPPDLDADIPSQPRLYTVEAWVPYLGEGDPRSWKQIDVYINGVDTPSDWNA
                     AMVPWSQPARTEWIARMPLPADEFEGRWVQAGPGPHSPDGPGCDAGAAGPYLWLKKGS
                     DLYMDFSWSGLKYGVSALRITEAFPVERTCDPPVSEGPVDLRCVGNSDPLYTIGEGFG
                     RGYEANIDGNTLAVENYRWPCISVGSQFDSNVLPLSPGLPYDVTHPEYAIYTAQLPTG
                     EVSFKLRYDGFNTMKWRTDAVGAFSKYNVFTLNEVPNREFISGRFSRLYVLTTKGGGS
                     SGKLRVEMVYSDGPSVVTEANLYDWFNQDGDAGSLAVGVDGRLRSESTDVIGFERLNS
                     DGLAPGGWGPANNGGDHGGAFLFVHPMDVDRSRMLTQIRIGLGDVENFGGELVVMGIT
                     LEVGCGDPVFDVAGDSGLELVGDGDVDQQDFAFFQSCYTGVGDPGGLFDRPRCGCLDW
                     DGDGDIDGDDFARLEQCASGPGVPAEPQCDD"
     gene            complement(13179..13316)
                     /locus_tag="P5316_13855"
     CDS             complement(13179..13316)
                     /locus_tag="P5316_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86088.1"
                     /translation="MTRAEKAVLFVIGMTPSGRAAEVFTDDFKDSASDLAVTADAIRD
                     G"
     gene            complement(13343..>13995)
                     /locus_tag="P5316_13860"
     CDS             complement(13343..>13995)
                     /locus_tag="P5316_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86089.1"
                     /translation="DPWGPPSRPNFDVHGWLVLTEPYRTEYYVLGVNSKQSWTHLAWA
                     TKTDGWNVTSAARMPGWRKMEIVVHPYTGAVGDVEFLVNDVVIAQGRRDSGSGSGIDV
                     SWLELGGDPALLTESHLTNTFEEFWYDEVALTFTPDPCSEVVMDADEDGDVDQADFGV
                     FQTCYTGSGNVGLFDELACHCMDADADKDVDGDDLVVFEQCASGPGIPADPACDGS"
BASE COUNT         2590 a         4561 c         4127 g         2717 t
ORIGIN      
        1 ggatactggc caaagccaag accacagcaa agtggtaccc ttgttctgcc cgctgtgggg
       61 cgggcagacg agaggaccta gcgacatgaa tgccaaggcc gtatggacgc tgatgaagga
      121 gcggcttccg gcctggaggc gcgcgcaaga caacgggcat cagagctcgg ttctccttcc
      181 tgccgacgag ccatctcccc ccaccaagat gcggccggca actggcattg tcgatgcctc
      241 cctgcccgac gatcagcgtt ggccggcgat gatgcgggcc ggcacccgac actacgagag
      301 tggtgaattc cgcttggccg tcgacgcctt ccgggccgcc gcccagttgg cccgcggctt
      361 cggttctgat gacccgcgcc tggcggtgag cctcaacgac tgggccgaag cttgccgtgc
      421 ctgcggcgac tgggcgcagg ccttgtcgct ctgccaggaa tcgctggcat taagaggccg
      481 cgttctcggg ccgtctcacc cccatgtcgg cgtcagcctg cacaacctcg gcaccctgtt
      541 tctggagacc ggacgactgg ctgaggccgc actggcattt gagcgagcct tgaccctgtt
      601 gcgcggcggt cttggttgcg gccatcccga tgtcgctgcc accctttgcg gccttgcggc
      661 ggtcatgaag gcagaaggtc atcacccgga cgccgccgag tacgtccgcc aggcgctggc
      721 catccgtcag gaagccttcg gctggaggca tcccgaagtg gcggcatgcc tcagtgcgct
      781 cggagacatc caccgcgaac tcggcgagta tgacaaggca cggaacctgt gcgagcaggc
      841 gttagccatg acccgcgaac tgctcggcca tgagcacttt gctgttgccg ccattctcaa
      901 caaccttgcg ctgctgaaca agacccaggg acggttggca gaagccgagc ttctctaccg
      961 tcaggccctg gccatcgagg agcgcacgtt gggcccgcag catccgtcgc ttgcagccac
     1021 gctcaacaat ctggcggtgc tgtgtttgtc actgcgacgg ctgaccgatg ccgagagtct
     1081 tctccggcgg gccctgtcga tctgccgtga caaatcgccg accaaccatc cggatgtcgg
     1141 cctgattcta gccaatctcg ccgagacctg ccgcctgtcc aatcggctgc cggaagcgga
     1201 gtcgctgtgc taccaagctc tttcggttgc ggaatccgct ctgccgccga accacccgca
     1261 tcgggcagca attcttaaca acctgggcgt cattctttgc gctcgcggcc aaaggaagca
     1321 atcgaaggtc ctcctcgaaa acgctgccgc cgtcctggaa cagcggccgg ccgacgatcg
     1381 ccttgcccac gtcgtccgac ggaatctgcg cgcctgagcc ccctacacag cgcgttgaga
     1441 acctggccat aactgcgggt gggtgctgct ttcgcaccga cgggcaggca gtcggcgaca
     1501 cacggcggat cagcaagact cctcaggtgg tgccggcatg aagggaaggc atgccgccgc
     1561 cctgccccgc cgcgaagcac ccgccggacg ctttatgacg cgttggcacg aacgatctga
     1621 acgagcggct tgacggtttc gcttcgcggc tcgggcggtg cgctcaatac cgggtgcacc
     1681 aggtgactgg gcgactcgtc atcagacgaa atgccttgcg gcatgatgcc gacccgcctc
     1741 cacgctgcca tctcagcctg cgacagtgcg tgatcgagtt tcggacggac tctctccgcc
     1801 ttggtcggct gaacgacggt cggcgccttc cgtgcgacct gtggtccgga cgccccctga
     1861 cggctcagtc gatcgtggag gcgggtgagg atatccatca aggtcctgcc cattgcggga
     1921 tccacgattc ccaggcggac ttcacgatac agccgatcca tcgcctcacc gaccgcggcc
     1981 ggcgtcgtga gcttgcgaag atagccgtcg ggcagatggc tcagccgtcg atggctcctg
     2041 ccgccccggg aggcgatttc gcggcgctgc tcggccgtca agttcgggtc atggaacgga
     2101 cagaagtcct ggcccgggcg agccctgccc ctgcaacgtc ttcccgaacg ggtcgtggcc
     2161 gcacattgct tctgcttggg ctcgcgagca tagatctgcg tcggctcaat ccgagccgcg
     2221 cctccctctt gtttctgagt tctgaatcgc aacgcttccc agaggagttc tgccgacatg
     2281 ttcctgcacc tcacgccgta caaaagaccg tcgtgaatct cgacgacttc gtctcatccg
     2341 cggccttgcg gccagcagca ctgcccagga ctcatcacca ggtgacatcc gtgccacgga
     2401 cttgccgaac atccggtcgg tgcctgttac gcggtatgaa accgcgttgg cggaaggctg
     2461 tgagaaaccc gcacagccgg tgattccggc attctaacga cccaccgcat ccagtcaacg
     2521 gcgagtgctc cgcttccggt cgcgcgcacc gccggaaaca ccatgaggcg cacgcaagtc
     2581 atgccatcta cggcatctgc gcgcaagatc ccacacagga ctctcggcga cgttctttat
     2641 gggacttctt gctgcgccgc ccgcctgccg cgaacgctac gagtgccaag tgaggtattc
     2701 acagggtgag ttgatgactg gagaccggct cgccaagcct gccacgcctg ttctgagctg
     2761 gacataagcc ggcccaattc cccgaccacg gaacccattt cccggttggt tgtcctgcga
     2821 gcggacgccc gctatagtcc cgacttcgcc ttctttttgg gagcgcaaac cgcccgtttc
     2881 aggaacgatg agatgctcca ggggatgttt gcatcacaat ccgggtcgct cagcgcccaa
     2941 ttcaccggtc tggattggct cgtggtgatc ggttatctcg ccctgaccac ggccatcggc
     3001 agcctgatgg ccggcaagca ggctaccatc cgcgattttt ttctcggcgg gcgcaagctg
     3061 ccttggtatg ccgtctgcgg ctcgaacatc gccactgaga tcagcgctgt gaccttcgtg
     3121 ggcgtaccgg ccatcgtgtt cgcctcccaa ggcaacttca cttaccttca acttcaactg
     3181 atcgggctgg tgctggcccg cctcatcgtt gcctatgtgt ttgtgcccgc ctattacgag
     3241 cgcgagatct acagccccta cgactacatg gccaaccggc tgggtgaaga ggcccgtacc
     3301 gtcaccacct gcctcttcat cctcggcggc tcgctggccc agggcgcccg cgtctacctg
     3361 accgccatga tcctggacat catcgcgggt aagagccttt acggcccgct ggcggacgca
     3421 acgggcatca gcacgctgac ctggtcgatc tacaccttcg ggatcatctc agtggtctgg
     3481 tgctggatcg gcggcatcac cacggtcatc tggacggacg tcattctctt tctggtgttt
     3541 gtggtcggag ctttcgtcgc gttgggtgca gccgtcgtca gacttcccgg cggctggtcc
     3601 gaattgatca gcatcggatg gaatgcaaaa caagccggtc gctggggcaa attcaccgtc
     3661 ttcgatttcg atctcagcct gatcaagccc tacactttct gggccgccct catcggctac
     3721 acctggcacg gcctgttcgc gttcggcacg gaccagatca tggcgcagcg catgttctgc
     3781 tgcaagggac cccggccggc acggtgggcg gtgatcggca gcaacctggg ccagatcatt
     3841 gccatcgtca tgctcatggt cggcgtcggc ttatatgcgt tttaccagaa gaacccactt
     3901 tccggggaag cactcgacct cgtccgaaaa gacatcaact acgtcttccc cgtcttcatc
     3961 attcaggaac ttcggcccgg catcaccggc ctgatcatcg ccgccatctt cgcggccgcg
     4021 atttccactc ttatctccgt cgttgcctca ttcagccaga ccaccctctc agccttctac
     4081 ctgccctggt atcggcgacg cagtactcgc aagggttcgc ccgtcggcga tgaacaggcc
     4141 atgcagaatc cgcggctggt gctgctctcc aggctgttgg tattgatctg gggcgcaatc
     4201 ctttgcctga ccgcccagct cgcccatgcc gccgcccagc actataaaca aatcctggat
     4261 cttgccctgg ccatggccgg ctatgcgggc ggggcgttgc ttgccgggtt catgttggca
     4321 ttcctcagga tcaatgtcga cgcccgcggc ttcatctggt cggccccgct gtcggtgctt
     4381 accgtactgg cggtggcctg gcaccagccg tgggcacacg caatttgctg ggcaggaacg
     4441 gtcatccttc tagccacttg gctgtttcac atctatggaa gaagaaaaga catgccggcc
     4501 ggcaagcaag cacccgaacc ggtactctct ccggctgtgc agaccgtcat cctggccgtc
     4561 ggcaccgcga tcatgatggt gatcagctac ttcggctact tcgagacggt ggacgccgcg
     4621 acgggcaaga tgacacgcac caccttggcc tggccgtggt tcatcgccat cggctcgctg
     4681 gtggccttca gcttcggctg gctgttggct ggaagaaaag acacggccgc ggcgcgagat
     4741 acggccgggc aacaaaatga cctgtccaac aacggggaca caagcggaac tgggtagggc
     4801 cggagttgaa ccggcgacac acggattttc agtccgttgc tctaccagct gagctaccta
     4861 cccgaaagac tgttccccga gggccgacgg gctcgctttg cggccttcgg gcacgctagg
     4921 ccccgccccg ccttggggta tttcatggta gcgtgcccgc atcaagcggt caacagggcc
     4981 ggccggacca acaggtccgc ttcttctcgg actttacgca aaggtggcgg cttacccggt
     5041 cggggttggc cctctgtgcg gcaagaggcg ggggtctgtc agccggaaac ccggatctga
     5101 aacagaaaca gaccggcggg acgggcgccg cccgcgcgcc ggcgatgagg ttgatgagtt
     5161 acccatagct cccgtgcgcc aacctgacaa agctcgccaa catgaaacgc cgtcaactag
     5221 ggcggtaaat cgtttgccct ccgaacgggc tttggctata ttgttggcag gtggagagcc
     5281 gatcccatgc taccgttcgc caagaaagga ccgtgtcgac ctacaatccg cgccatcgcc
     5341 gagatggctg gtgtctcgcg caccacggtc tcgctgatct tggccaaccg gcgggacatg
     5401 attgaccgtt ttcgcccgga aacggtcgat cgtgtgcgaa aagccgccga aacccttgga
     5461 taccgggcca acttgctggc gctcagtctc cgaagccccc gtccgtcgtt tttcgccctg
     5521 gttctccgag gccccggcca agtcgacccc gattcggttt catggcatca tcaggctttt
     5581 gaaggcatgt ttctggccgg agcgctcgag gcctcacggg ctctccgatt gtatcctgtt
     5641 gtcgccacgc aagatctgcc caaaacagaa gattcgcttc ttgctttgac cgaggtgctg
     5701 gagggcggcg ttttcggcgc cgtgatccgg tctccggtcg cggttttgca cgagccgttg
     5761 agccgtcaca ttcaacaagg catgccgatc gtgacggtat tcccggagcg tcccgacctg
     5821 taccccacga acgtcattga cttggacaac gccgccacgg gcgaactggc aggccgtttg
     5881 ctccacgagg cggggcggcg ccgctggctg tttctgttgc aggcagatgt ttgggaggcg
     5941 ttacgcatcc ggctggcagg tcttcggcgg tccgctgaac aagcgggatc atcgctggag
     6001 gagctggttg ttccggccca ccttgcggaa gctgacgtgc ccggttggct ggccgcgagg
     6061 ctcaaggata tgaagatcga cggggtctac gcaccgtcgg ctctctcagc ggtgcgagcg
     6121 ctttatgcct gcacggccgg ggggctgaga gtccctgagg acgtctgcgt cgtcggcacc
     6181 gatgccgcct tgtggcgagc ccgcgggttt ccgatcatca cgtcgataga cgtttcgtgg
     6241 ttcagggcag gcgatctggc agtccgccga atggcggaac tacgagacag tggagaatcg
     6301 ttcttcgaga acgtctgcct cccgccgatt gttcatgcgg gcgacagttg tccgagcaac
     6361 ctgagcggac cggtcacggc aactgccggt gaaccacagc gggtttgagc aggcgcgtca
     6421 attcgatcca tgattcgcaa ccgattgcat accactccgt gatcctggtt gcctccgtga
     6481 ccgacgaaac gattcgcctc cacccgcaat ggtgcgatac ggcgtcagcc gtcgcgcgtg
     6541 taccaccccg aaaatcatac cgaatcgctc aacgagctga cctatgggtt cgacatgcag
     6601 tcgggctcgg cgggaatacc cgaacccgat aggcacccga tgaaaatcgc catatcgtcg
     6661 gcgtccacgt cgctgtcgtt atcgagacgg gcatcctgac agtcgggcaa aatctgggcg
     6721 acttgtgagc cgctgagaca caattgcagg tgcgcgaaat ccacctgatc cacatccgtg
     6781 tcgtcgtcgt aatcaacgac cagaatcgcg ccgggaacca catgagcgat gaaattgtcg
     6841 actcgggcgc ctcgcatgtt ggcgtcggta ttgaacatct catggtagcc gacgccgtaa
     6901 taaccgcggg gatgtgaact gtcgcgcacg gtgcagatgg cggtgtcatc cagccgatag
     6961 cggatacgat cgccgtaggc gtcgattcgg aaccgatgcc aaccggtcag cggcgtcccg
     7021 ggcacatcgg ccgcaaagtc ggtccacatg ccgtttacgt acttgcctgc cttgatcaag
     7081 ccggtatctg aggcatacag aagggcatag cagttgccct tcccgaagct ggacagtccc
     7141 atcgcgccgg tgccgctgtc ccgggcaatg atgccgctca gctcgtaccc gtcggccgcc
     7201 gcctccgggc ggtattcgca gtagacatcg gcctccaccg tgtagtcggt gtcgccgaag
     7261 tcaccccgcc ggacggaatc ggccccgccg tacctgttca tgaccttcag cacgtatcca
     7321 tcaccaccgg gtgacgtcgg cgagaaagac acaacgtctg cataacagaa cttgtcatcc
     7381 catccggcca gacgcccggc gggtccgaat gagtccgtaa aaggaaacac gctcgcggtg
     7441 ctctgtttga ccaacatgat cgcgttggca acggggcgct cgctgccgtc cgaggggacg
     7501 tttcggatcg ccccgttgac aaccatggtc gatgagccgc cgccgtcgta gttgactgct
     7561 tccgtcgccc cgagcgtacc gaccagaaaa ctggccattt cctgaaaggt catgccgacg
     7621 cttgcggccg accggccgtc gcacaccacc tggaaccagt aactatcatt ccaggccaag
     7681 accgtccgcg ggtttcggcc gtaaggcgcc gcgcccgatt ccagattcgc ccatccgggc
     7741 gtgtaagcag cgccattgcg aatgacccac ccgatgcccg ttaaagcgtt gtccgagtcg
     7801 ttgaactcct ccgcatcggt cgcgaaccgc atccgaagac ggtcgccgac gtgggcgtgg
     7861 ctgacaatct cgctcttggt gctgccccac gcggacagga ccatgccccc gaccggaatg
     7921 gcggcattcc cttccgtggg cgtgtgcacc gcgctgatga ttcccgacac ctccttgtgg
     7981 ctgcgcatgg gatagctgac gtcgttcagg acgacttcca ccgccatgga gggagtgatg
     8041 aaactgctgc gcgttgagct gtcaaagtgg ggcgtgaacg ccgccacgcc gttgatcggg
     8101 accagcggcc cgttcatgta gaaattgtac tgattcagcg gcatcgagtg gccgtcggcg
     8161 aaggtgagag tgccgagcac gtggttgaaa ttcgtgcgaa ccaccggccg ccgcgacggg
     8221 ccgaccatga cggtgtgata ctggtacgag ctgttgaacg ccggggtatc cagcatctgg
     8281 ccgtcggact ggccgatgcc gatcaatcgg ggaaggttaa cgccgtcgaa atacgagccg
     8341 ttgaccgccg atagtacgtc gtgttccggg gggttgtcgt agcgaccggc aatcgtgctc
     8401 gtcccctcac gagcagtgta gttgcgctta ccctgggccc agcccacctt gagcttgtat
     8461 tctgagtgga gccgctccgc cgccacaacg tggaccatgt tgggaccggc aacgttgtag
     8521 accgtgtagg tgacgcccgg ggccaactcc tccgcttcgg ccggtgcggc cgagaccaca
     8581 accccgacca gtccgggcag catggcgacc aatcgcctca ccaacttgtt cccgagcgca
     8641 tccacgtccc ccccactccc ggacccgcgc cgctctcagc ccgatcaagc ggcggcaaca
     8701 ggtcaatcaa agctgatatc gcctttccgg ctgccaaacc acagcgggat cacaggcgag
     8761 ggcttgttgg tcggtacggt cgggtgcatc ctgaacattt cccaccaacc cattcgctcg
     8821 acatgggcgt ccagaaaacc ggcgttcatt ctctcccgcg gttgatagtc caagtcccgg
     8881 gagttgccaa gcgatccggt ctgcggcggc gcagccggcc aggcaccgcc cgtatagttg
     8941 ccaatccatt ccgcgccttc gatgccctcc ctcttgacaa ggccgccgtg tcggaaccgc
     9001 agggccctcg cgaggttgtc ggatgggtag ttggaaccca aggcctcggc gaagatgccg
     9061 aatttcgccc cttcgactat gaggacgggg gtacccctgg catttttgaa gcccgcgctg
     9121 gcattcgccg aaaagctcat ccaaagaatg ggcacggtct tgatcacccc gctggacgac
     9181 acgttctggg cgatgatctc acccttgtag gtcatgacat catgacgcca cgcggtggag
     9241 ccgggcttcc acatcttggc cggagcaaaa gtctcgcgac tcgcaaccgt gtattgtatc
     9301 agaaagtccc cgtcgttgtc cgagtaagca tcgccgccga agagcgtttc atccacttgg
     9361 tcttctatgt ccgtcgtcca ggtgctgcca ttgcgagcca gacgacagaa gatggcatcg
     9421 ccggtagtga cgccacggta gtccgtcccc gccgcatcgc tgtacagcct gtcctgcact
     9481 gcgggtatgg gacgcggctt ggggtcagat gggcaggtga acaactccgt ttgccctttg
     9541 ttctgcttga gcgagtgcac ggcccagccg aaattcgtgc tccagtactc tttgcgcatc
     9601 tgcgtctcgg cctgccgcaa cacgacgccc tgaatgccct ggcgcatgtt gctggcgcac
     9661 acggcggcct ggacctcctt gcgggctctt gacaacgagg gcagtagaat ggcaatgaga
     9721 agagcgatga tcgcgacaac gaccaggacc tcgatcaacg taaatccggt tcgcaaaggg
     9781 cttttcatgt gtctgctccc gacggcacag gggacgggtc gtagcaatgc taccacccct
     9841 gtattgtatc cctgccctga tgctgcctca aggactgcgc cgatatcttg ttgatttcac
     9901 cgtcgtggct cttacgatgc gccgggaggc atctgcccca ctcggcggca agcgcaagat
     9961 cggcctcagt cgtcacactg cggctcggct gggacgcccg gaccggaggc acattgctcc
    10021 aacctcgcaa agtcgtcccc gtcaatgtcc ccgtcaccgt cccagtcgag gcaaccgcat
    10081 cgggggcgat cgaacaggcc gcctgggtca cccacacccg tgtagcagct ctggaagaat
    10141 gcgaaatcct gctggtcaac gtcgccgtca cccaccaatt cgagaccgga gtccccggcc
    10201 acgtcgaata cggggtcgcc gcaccccacc tccagcgtga tgcccatcac gaccagttca
    10261 ccgccgaagt tttcgacgtc gcccaggcca atcctgatct gggtcagcat ccgagaccga
    10321 tcgacatcca tcggatgaac gaagagaaac gcgccgccgt gatcgccgcc gttgtttgcg
    10381 ggtccccatc cgccgggggc cagcccgtcc gaattcagcc gctcaaagcc gatgacgtcc
    10441 gtgctctcgc tgcgcaggcg cccatcgacg ccgaccgcca gggaaccggc gtcaccatcc
    10501 tggttaaacc agtcgtacag attagcctca gtaactacgg aaggcccatc gctgtaaacc
    10561 atttcaaccc gcagtttccc ggagctgcca ccgcccttgg ttgtcagaac atagagccgg
    10621 ctgaaccgtc ccgagataaa ctcacgattg ggtacctcat tgagggtgaa cacgttgtac
    10681 ttgctgaagg cgccaactgc atcagttcgc cacttcattg tattgaagcc gtcatagcga
    10741 agcttgaacg aaacctcgcc cgttggtaat tgggcggtgt agattgcgta ctccggatgc
    10801 gtcacgtcat acggcaaccc cggtgaaaga ggaaggacgt tcgagtcgaa ctggctgccc
    10861 accgagatgc agggccatcg gtagttttcc acggccagcg tgtttccatc gatgtttgcc
    10921 tcataacccc gcccaaaacc ttcgccgatg gtatacaacg gatcggagtt gccgacacag
    10981 cgcaggtcaa ccggtccctc tgagaccggc ggatcgcacg tccgttcgac gggaaaagcc
    11041 tccgtgatcc gcagagccga tacgccgtat ttcagcccac tccagctgaa gtccatatac
    11101 agatcgctgc ccttcttgag ccagagatac ggccccgcgg cgccggcatc gcacccggga
    11161 ccatccggag aatgtggccc cgggccggcc tgcacccagc ggccctcaaa ctcatctgcc
    11221 ggcagcggca tgcgagcgat ccactcagtc ctggccggct ggctccaggg caccatggcc
    11281 gcgttccagt cagagggcgt atccacgccg ttaatgtaaa catcgatctg cttccatgat
    11341 ctcgggtccc cctccccaag gtagggaacc caggcctcga ccgtgtagag acgaggctgc
    11401 gagggaatat ccgcatccag gtcaggaggc gtgcccccgg gaacgtccga ccgctcaact
    11461 cgccaatacg ctcgaggcca agtctcatcg gcgttgtatt cccgccagta gtagcggccg
    11521 tccaatgggt aactcccaag atggattccg tcatcgtacc caccggttgg aaccatatgc
    11581 cagccgcttc cctgaagctc gcgaatgcgg agcacccaac tgcgcaaccg gctaacgctc
    11641 accgagacgc cgtcggggtc ggtgaggccg ccgtcgttcg tcgaggtgat cgtcgcctcg
    11701 cccaggacac caagccgaac gttagcggtt tgttgagtgg caccgccctg aggaaaactc
    11761 agcacgagaa cgccgccgct gccgccggtg aaatccgcga ccgccgggtt gtcgctggtc
    11821 agcgtgactt ctacggtccg agtatcgttg gtgcccgggc tgatggacac cgtaacgggg
    11881 cgagttgtgc ctggggtacc ggtcagagac gccggcgcca gcgtcacggc accggtctcc
    11941 acagtaaccg gcaacgtgtc gtccacgagt tcggcgtcgt tgttgcttga aatggtggcg
    12001 gtgccggcct ggccgatgtc gatctggata ccttgctcgg tagtcccgcc ctgcggaaag
    12061 gtcagcacca agacgccgcc ggtcgcaccc accaggtcgg ccacggccgg gttgtcactg
    12121 gtaagggtaa cttggaccgg cgcatcagcg ttgctgtccg ccgggatctt caccgtcccg
    12181 cttacggtcg cgccaccgac cacagtgttg atcacgccag gtgagatgtt gacggcatta
    12241 ggcggacctt cctccacaag catgagcccg gccgggccgt aaccgctgtc cgctttcatg
    12301 cctgcccggt acgcctcggg aacttcggcg ggggtggcgg cgcggaagtg aacgctgaac
    12361 ttgccgtccg ggcccggcgc aatacccgtc cacatggcca cgaacccttc gaccgtgttg
    12421 taaccgctgt cctggctgac cgaggtagga ctgacctggt aattgccaac gccgacgctg
    12481 catgcatagg tggcaatagt tgaatcagcg ttgaccagcg agatgttggc ccaccgctta
    12541 ttgtcgtaac ttggttcgcc acggtcggtg aaggtcgcga cggtataagt tttgttgacg
    12601 tccaggccgc tgaattccat ggtgaaatac cacccggtcg agggcgccat caccttgccc
    12661 cgaccggcca ccttgccgcc gaaaatcgtt aaggcgtccc cgccgctgat ttcagcgctc
    12721 ggaccagtgc catctgtcga gagaccggac cgggttatgg tcatggtcgg cgtgacggtc
    12781 gcacccgtgg cataatcctt cagcgcatac gtgccggcgt tctgattctg cgtcacgttg
    12841 gtggcgccgg caacgtaatc cggcacaaag tcgttgtaag cggtgaactg tgctcgggcc
    12901 gcaagagtcc atcccaagaa caccgcaatg accaggatgc cgcaaaaccc gttcttgatt
    12961 cgacacgatg tatgcatgaa aacctccttc ccggtatctg cgccggttcg ctgattcatt
    13021 cacacaaaag ggtcactgtg acacttgctc cgtgactcca ccacacgaat ctctacggtc
    13081 gcaaccgggc gcatttcggc gcacgtttct ggatcgcagt gcggcttggt tgcggtcgcc
    13141 tcacgccggt tcggcagaac cccgagcacc ggcgatgctc agccgtcacg gatcgcatcg
    13201 gccgtaaccg caagatccga ggcactgtct ttgaaatcgt ccgtgaagac ctccgcagcc
    13261 cggcccgacg gagtcatgcc tatcacaaac aggaccgcct tctcagcccg ggtcactata
    13321 ctcctctctt gcatcgtcct tgctaggagc cgtcacacgc cgggtccgct ggtatccccg
    13381 gccccgaggc gcattgctcg aaaaccacga ggtcgtcacc atccacgtcc ttgtccgcgt
    13441 cggcgtccat gcaatggcat gcaagttcat caaacagacc cacattgccg cttccggtgt
    13501 aacacgtctg gaagacgccg aagtccgcct ggtccacgtc cccatcctca tccgcatcca
    13561 ttaccacctc ggaacaggga tccggggtga aggtcaacgc gacctcgtcg taccagaact
    13621 cctcaaaagt attggtcagg tggctctcgg tcagcagggc cgggtcgccg cccaattcca
    13681 gccaactgac gtcgatgccg ctaccggaac ccgaatcgcg acggccttgg gcgatcacca
    13741 catcgttgac cagaaactcg acgtcgccca ccgccccggt gtacggatga accacaatct
    13801 ccatcttccg ccagcccggc atgcgggccg ccgaggtcac gttccatccg tccgtcttcg
    13861 tggcccacgc cagatgcgtc cagctctgtt tcgagttcac acccaggacg tagtattccg
    13921 tgcggtatgg ttcggtgagc accagccatc catggacgtc gaagttgggg cgagaaggag
    13981 gcccccaggg atcac
//
LOCUS       DAOTPW010000150        13881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4317_length_13881_cov_4.920223, whole genome shotgun sequence.
ACCESSION   DAOTPW010000150 DAOTPW010000000
VERSION     DAOTPW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4317_length_13881_cov_4.920223"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1269)
                     /locus_tag="P5316_13865"
     CDS             complement(214..1269)
                     /locus_tag="P5316_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR86090.1"
                     /translation="MKPSARTGFTLIEVLVVVAIIALLVAILIPSLINARNLAQRSVC
                     ASNLRQGLTGVLLMQAETQMRKEQWSLNYGWAVDSFKRNAGAPNIFLCPADPDPKPIP
                     AVYDEQYGHQGSEGISSGASIFNRFRRSGDNWTFDFQDQVLGNMFGGDSYSEPTGDCL
                     MDFTATPGQTTTTATVRRDVTYYDHRGYSYRGEQLWTNTASNGPITVPLFWSSFGANA
                     SAGLRNVKGSPIVVVEAGKLGVFAEDFGATSQGSRPRDHLGKVLRFRHGGKAVRPFLG
                     GRGSDFTKQFHLPTQAVDGLYEPRTRANSGFMDGHVEALAYYQLFQADPSNPENAPTI
                     TRSLWIGSRKGGPEQGY"
     gene            complement(1639..2634)
                     /locus_tag="P5316_13870"
     CDS             complement(1639..2634)
                     /locus_tag="P5316_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRR86091.1"
                     /translation="MRRSYWLILATGAVFIAAVGLRVRSHLVASGQPPAVTSRPAGRA
                     LRISEPRDYVANPWVDPSERDKGPNRLISLAPSITEIVCALGLRDRLVGRTPYCTHPP
                     GIERVADVGSLVTVNLDMIKTLKPDLILVTRNSGQVIDALGKLGLPHAALPHDTLEEV
                     YTAIERIGSLCGRPKTATALAAFIRNDLETLRQTARRLDLPRRRVLIILGDLPVPPRP
                     VWVAGPGSFLESLLQAAGQQNAAAGAMPVSHGEIGLERLLTLDVEAILTFGEPLTDRQ
                     WDDLYRTWAKVGGIPAIRERRVARVGGSEWLSAGPRVAIALHHFITALAEGEGTN"
     gene            complement(2676..2987)
                     /locus_tag="P5316_13875"
     CDS             complement(2676..2987)
                     /locus_tag="P5316_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HRR86092.1"
                     /translation="MAMLLEFSMFPLGKGESVGEYVARSLDVIDRSGVPYQLNAMGTV
                     LEGEWDQVIGVVGQCLERMSQDCDRIACTIKFDWRKGATGRLQSKIDSVEKRLGRKLK
                     T"
     gene            complement(3126..3620)
                     /locus_tag="P5316_13880"
     CDS             complement(3126..3620)
                     /locus_tag="P5316_13880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR86093.1"
                     /translation="MVNLHIREMTETDIPRVSELLRDCFRWLGDREHLTARQLAFLVD
                     DRSSEQTVREESKSRPHLVACTETGIVGMAAVHGNEIARLYVDPRYHRQGVGKALFEA
                     CEAMIRRAGHEEMTVAALVDGALAFYQTMGMSITGRVVYEPEIFLGREVTLLAKKVAS
                     APMP"
     gene            complement(3810..4823)
                     /gene="fba"
                     /locus_tag="P5316_13885"
     CDS             complement(3810..4823)
                     /gene="fba"
                     /locus_tag="P5316_13885"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473872.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HRR86094.1"
                     /translation="MPLVPMRILLDHAAENNYGIAALNVNNMEQIQAIMEAAKETDSP
                     VIVQASRGARKYTNDAYLRHLMLAAVELYPKIPIAMHQDHGNSPATCKSAIEQGFTSV
                     MMDGSLSEDGKTPSSYEYNVKVTREVVQMAHAVGVTVEGEIGVLGGIEDGHGAGGTGL
                     EHVTDPALAEKFVADTGVDALAVAIGTSHGAYKFTKKPTGDVLKMDIIEEIHRRLPNC
                     HLVMHGSSSVPRELVDLINKYGGKMKESYGVPVEEIQRGIKHGVRKINVDTDNRLAIT
                     AAIRKVFAETPDKFDPRDYLGPAREMMKQICIARMTAFGQAGNAGKIKIKTCKDMISF
                     YKK"
     gene            5500..6147
                     /locus_tag="P5316_13890"
     CDS             5500..6147
                     /locus_tag="P5316_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86095.1"
                     /translation="MKMLLGSIAVMLCIPALVWADAPPGTIVYIEDDFLPDQDMNGWT
                     TYGNATIGNPSGNGFSLWGPCYRRADEQDGIAGIQEGGGGGRSFGMYKRFYDEYIPEG
                     EKHIQAWVRMLSCTWGWELTPGALNVRLGKDPLGGTDPNAPSVIWTAPYWGDWNRLEL
                     IQPCVEGWETIFIQVDGFMNGYYQILVDTVEVWQTPEPAAAALLMLGLPLLRRRR"
     gene            6525..8351
                     /locus_tag="P5316_13895"
     CDS             6525..8351
                     /locus_tag="P5316_13895"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86096.1"
                     /translation="MKWWQRLALLGFSFILLVLMELALRVVGYGPDPDLMVRRAEVEG
                     GRIIGLNPVAWKRFALRPTPHRPGEGVLKVHDFRDPKPPGIFRVFFLGESSVQGVPYE
                     RNATMCAFLEALLAAAWPEIRVEVINCGVTAVNSYSLRAWLPEVLEYEPDLLVVYAGH
                     NEFYGFYGPGSLHGVGTSRKAVLTHMWIRQLRLSQVIRDGLDTFRSPPPPESAATLME
                     MMAKDRLIRLGSGVYSACRENFRANLNDMVQAAQGAGVPMVLCGLVSNERDLVPMVSA
                     NREGLSRQQEDQWRRDFQEGAGMVSAGRWGEALPLLERAAQIDDTHAELVYRLAQCQQ
                     HLGQPHEARALYRRARDLDCLRFRATGEFSEVIRSVAGKRCIFVDTAAAFEAASPDGL
                     IGWNLMTDHLHPTVYGHYLIARSICHALAAECERLGLRAIEPDSLPQFERAAEVLGCD
                     QLTELIGSLHILKLMQAYPFAGTPNAARAAELLTSTARDMASLPQEIVPVIDQWIKGG
                     SKGSLHFAVARLYESSGQTDKAMPYLRRAERTTEPHSPEAVEIKLEMARFLAASTRPE
                     DAERAAEYAQQALQYVEESARVHPEAKSRLAAASAEIRQMIR"
     gene            8392..8571
                     /locus_tag="P5316_13900"
     CDS             8392..8571
                     /locus_tag="P5316_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HRR86097.1"
                     /translation="MARIQRHDGKLSTLRELLAFMWGHKRWWLLPVAIVLLLLAGLIV
                     FAESSALAPLIYTVF"
     gene            8587..10401
                     /locus_tag="P5316_13905"
     CDS             8587..10401
                     /locus_tag="P5316_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HRR86098.1"
                     /translation="MHILGISAFYHDSAASLLRDGRIVAAAQEERFTRRKHDASFPRE
                     AIRYCLREGGIGGDQLDYVVFYDKPLLKFDRLLQTYCSFAPRGIRSFLTAMPVWLKSK
                     LWLRNLIREELAGCGEILFCEHHGSHAASAFFPSPFERAAILTIDGVGEWTTTAWGVG
                     EGNRIRIDAEICFPHSLGLLYSAFTYYCGFRVNGGEYKLMGLAPYGEPRYVDLILERV
                     MELKEDGSFRLNLDYFNYCTGLTMTSRRLHRLFGGEPRRPESEITQRDCDIARSVQYV
                     TEEAMLRMARHVHEQTGCRHLCLAGGVALNCVGNGRILREGPFDDIWIQPASGDAGGA
                     LGAALSVWHQYLGRERETGGGADGMQGAQLGPCFVTDQIERFLEQEHIPANHYSFSEL
                     AQRAAELIASGHVLGWFDGRMEFGPRALGNRSILADARKPEMQATLNLKIKFRESFRP
                     FAPAVLLERVTDYFELDRPSPYMLLVARVRESHRRLPSESERREGLDRRKEIRSDVPA
                     ITHVDCSARIQTVDVRDNDRFHRLLCAFEHLTGCPMVINTSFNIRGEPIVCSPSDAYR
                     CFMRTQMDYLVLGDYLLAKGCQPESKRQSIPSPAEIID"
     gene            10404..10829
                     /locus_tag="P5316_13910"
     CDS             10404..10829
                     /locus_tag="P5316_13910"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HRR86099.1"
                     /translation="MRGCGIHGEPSQRAELRRFGAVLGLALIILAVVFCCRRHSLYLP
                     AAMAVAGSVLMVSGLAFPLVLLPVRRTWAMLGAGLGWLNTRVLLTIVFYAIVTPTSLL
                     LRLARRDPMNRRWREPGTSSYWVRREPKPFESGDMRRQF"
     gene            complement(10924..11625)
                     /locus_tag="P5316_13915"
     CDS             complement(10924..11625)
                     /locus_tag="P5316_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86100.1"
                     /translation="MCKLVCLALYLVSAASAVAGTLGLANEIANGDFETGGWTPWTQS
                     SFGLFNQGQDDVPVNPAGGQKWAGQILRWNGNHNEPAGVLRQVMDGTQSPGWEPAGTG
                     KLIDLEFDYLLDSVGSDAQRKVGLLVYLHWQGDGSEPPAPDGLDCHRHLVFEEWRYPD
                     TGAPEWKHADIRLELPFQPRFLELAFESHVHFPKLGVVGIDNVDLESRCLPEPFSLAL
                     LASAVILVLRRRQQA"
     gene            complement(12032..12955)
                     /locus_tag="P5316_13920"
     CDS             complement(12032..12955)
                     /locus_tag="P5316_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRR86101.1"
                     /translation="MRVLITGSSGQIGTNLALALQARGDQVVGIDCRPNSWTDEIETC
                     LIDLNTAREKDYPAGPFDLVVHLAAYAKVFELVEHPERALENVTMCFKVLDYARKHRL
                     PLIFGSSREVYGDIRRHVTEEAEADFVVAESPYSASKISGEAFIYSYAECYGLPYLVF
                     RFSNVYGRYDNDIERLERVLPLFMRRIARDEPIIVYGKEKVFDFTYIDDCIDGIRRGI
                     DGLLAGRVVNQTVNVAYGQGSTLVDAVNIISLALGKTPKVSYEPARPGEVMRYVADIS
                     KARELLGYDPQTPLTAGIPLAIAWARQRGEL"
     gene            13194..>13881
                     /locus_tag="P5316_13925"
     CDS             13194..>13881
                     /locus_tag="P5316_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86102.1"
                     /translation="MEHLSHLLDYVAGLEYFRYLNGVDYAVMTVYAAIVVGIGVYLKR
                     RASRSLEHYFLAGRQLPWWALGVSGMLNFLDMTGTMLIVSFLYMLGPRGLYVEFRGGA
                     VLILAFMMLWVGKWHYRSKCMTGAEWYVYRFGSDNMAKASRLIGAIGGIVPSIGMLAY
                     LMQGAGTFLSMFLPFGPKTCAVLMVALTVAYTLMSGFYGVVYTDLFQSFIILVAVVII
                     SVLAVTHVVGY"
BASE COUNT         2509 a         4113 c         4368 g         2891 t
ORIGIN      
        1 ccatcttctc ttggcgtcat ggcggtccca ttcgctcgcc ttttacatcc gtgcgatcgc
       61 tgtgatctgt ggtcacactt cttgtagccg gccgccccgg ccttgtttta tccttcatcc
      121 ttccgccttc atcctatctt ccggcacctc cgtcccatcc ggccaattcc caattctcaa
      181 ctcgtaattc ctaccggcac tactccccgc tcgtcagtat ccttgctcgg ggccgccttt
      241 tcgtgacccg atccaaagcg aacgagtgat tgtgggagca ttttccgggt tggagggatc
      301 ggcctggaat aactggtagt atgccaatgc ctccacgtgc ccgtccatga agcccgagtt
      361 ggccctggtc cgtggttcat agaggccgtc gacggcctgg gtgggcaggt ggaattgttt
      421 ggtgaagtcc gagccgcgtc cgccgagaaa cggcctcact gctttgcctc cgtggcggaa
      481 gcggagcacc ttgccaagat gatcgcgggg gcggcttccc tggctggtcg ccccgaagtc
      541 ctcggcgaac acgcccagct tgccggcctc caccacgaca atcggcgatc ctttgacatt
      601 gcggaggccg gcactggcat tggcgccgaa gctgctccag aacagcggga cagtgatcgg
      661 accgttcgag gccgtgttgg tccacagttg ctcgcctcga taggaatagc ctcggtggtc
      721 gtagtacgta acgtcgcgcc ggaccgtcgc cgtggttgtg gtttgtcctg gggtggcggt
      781 gaagtccatc aggcagtcgc cggtgggctc cgagtacgaa tcgccgccga acatgttgcc
      841 aagaacctga tcctggaaat caaaagtcca gttgtcgccg ctgcgtcgga accggttgaa
      901 gatggatgcg ccggagctga tgccctcaga gccctggtgg ccgtactgct cgtcgtagac
      961 cgccgggatg ggcttggggt cagggtccgc agggcacagg aaaatattgg gggccccggc
     1021 gttgcgtttg aaggagtcaa cggcccagcc atagttcaga ctccactgct ccttacgcat
     1081 ttgcgtttcg gcctgcatca ggagaacccc ggtcaggccc tgtcgcagat tgctggcaca
     1141 caccgatctc tgcgcgagat tgcgtgcgtt gatcaaggaa ggaatgagga tggcaacgag
     1201 gagagcgatg atcgccacga cgaccagcac ttcgatcaac gtgaaaccgg ttcgagccga
     1261 cggtttcatg cgtcctctcc ggatgccgag gtgaccgggc tctgcaagat accgtccccg
     1321 ccgcaagtcc cacgctcagg cgcgcagaaa acggggggcc ttctcccacc catcatgtcc
     1381 gattcaaggg atctccgtcc agccccgcca tcggttctgg ctaatgaaca ttatacccgg
     1441 cgtgaggaag atataagata gcacagtccc gcaagaaggc tgggacaggc ctgacccccg
     1501 gggccggcag cccgagttgt cagtcttcag ttctcagtcg aggcagggcc ggtgtctccg
     1561 cccaactgac gacggatcac tgagaactga ccattcgctt tcccatccgg tcaattccca
     1621 attctccaat ctcaattctc aattcgtccc ttcgccctcg gccaaggcgg tgatgaaatg
     1681 gtggagggcg atggccaccc gcgggccggc gctgagccac tccgagccgc cgacccgggc
     1741 gacacgccgc tcgcggatgg cggggatccc cccgaccttt gcccacgttc gatagagatc
     1801 gtcccattgc cgatcggtca gaggctcgcc gaacgtcagg atcgcctcaa cgtccagcgt
     1861 cagcagccgc tcaaggccga tttcgccgtg cgagacgggc atggcccccg cggcggcgtt
     1921 ttgttggccc gccgcctgca agaggctttc cagaaacgaa cccgggccgg ccacccagac
     1981 cggtctgggt ggaacgggta gatcacccag tatgatcaga acgcggcggc gaggcaggtc
     2041 caaacggcgg gccgtctgac gcagcgtttc caagtcattg cgaataaacg cggccagcgc
     2101 ggttgcggtc ttgggccggc cgcacaggct gccgatgcgt tcgatggcgg tgtagacctc
     2161 ttcaagcgta tcgtgtggaa gagcggcatg cggcaggccg agcttgccca gggcatcgat
     2221 tacctgtccg ctgtttcgcg tgacgaggat gaggtcgggc ttgagggtct ttatcatgtc
     2281 gaggttgacc gtcaccagcg aaccgacatc ggccacgcgt tcgatgcccg gcggatgagt
     2341 gcagtagggg gtacggccga ccaagcggtc gcgcaacccc agagcgcaga cgatttcggt
     2401 gatgctcggg gcaagggaga tcagccggtt cggaccctta tcgcgttcgg aggggtcaac
     2461 ccacgggttg gcgacgtaat cgcgaggttc gctgatgcgg agggcccgcc cggccggccg
     2521 gctggtcacg gccggcggct gcccgctcgc caccaggtga ctgcgaacgc gcagccccac
     2581 ggccgcaata aagacagccc ccgtcgccag gatcaaccag taagaacgtc gcacgccgac
     2641 ctcaccagcc tgctgcgggc cccagattcc cgtccctacg tcttcagctt ccgtcccagc
     2701 cgcttctcca cgctatcgat cttgctttgc aggcgtccgg tcgccccttt gcgccaatcg
     2761 aacttgatcg tacaggcgat ccggtcgcaa tcctggctca tcctttcaag gcattgaccc
     2821 acgaccccga tgacctggtc ccactcgcct tcgaggacgg tgcccatcgc gttcaactga
     2881 tacggcacgc cgctgcgatc gatgacatca aggctgcgag cgacgtattc accgaccgat
     2941 tcccccttgc ccagcgggaa catcgagaat tcaagaagca tggccatggt gcgtactcct
     3001 ctctgagaat gccgggcatg cccgtctttc ttgagttcgt atcataccac cagattggtt
     3061 tcgcaggacc gttgtcaggt gcccgcgcgg gacaagacgg gtccgcgaca ctcgcggcgg
     3121 ctctgtcatg gcatcggagc agaagcgacc ttcttggcca gcaacgtcac ctcgcgaccg
     3181 agaaagatct cgggctcgta caccacccgg ccggtgatcg acatgcccat ggtctggtag
     3241 aaagccagcg ccccatccac gagggctgcg acggtcatct cctcgtggcc ggcccggcgg
     3301 atcatcgctt cgcaggcctc gaacagggcc ttgcccaccc cttgccggtg gtaccgcgga
     3361 tcgacgtaaa gccgggcgat ttcgttaccg tgcaccgccg ccatgcccac aatgccggtt
     3421 tcagtgcagg cgacgaggtg cgggcgggat ttggattcct cgcgaaccgt ctgctccgac
     3481 gagcggtcgt cgaccaggaa tgccagttgc ctggcagtca agtgttcgcg gtcgcccagc
     3541 cagcgaaagc aatccctcag cagttcactg acgcgaggga tgtcagtctc agtcatttcg
     3601 cggatgtgca gattcaccat gatccgggcc ggtttcactt ttccagaacg gcaatcttta
     3661 ccgcaacaga atagccctgt ggcgcggacc cagcaacaag aatagtccgg cccggaagaa
     3721 gcaaacccac ggtttaccac cgcgggtttg cggaagcctc gtgttttttc tgatccggcc
     3781 gcaaccggcc gaagggtgac aaaggacgct cacttcttgt agaaactgat catgtcctta
     3841 caggttttga tcttgatctt cccggcgttg ccggcctgac cgaaggcggt catgcgagcg
     3901 atgcagatct gcttcatcat ctcgcgggcc gggccgaggt aatcacgagg atcgaacttg
     3961 tcgggcgtct cggcgaagac cttgcggatc gcggcagtga tggccaggcg gttgtcggtg
     4021 tccacgttga tctttcggac gccgtgtttg ataccccgtt ggatttcctc gacgggcacg
     4081 ccgtagcttt ccttcatctt gccgccgtat ttgttgatga ggtcgaccag ctcgcgaggg
     4141 acgcttgacg atccgtgcat gaccaggtgg cagttaggca ggcggcggtg aatctcctcg
     4201 atgatgtcca tcttgaggac gtcgccggtg ggtttcttgg tgaacttgta ggcgccgtgg
     4261 ctggtgccga tcgccactgc cagggcgtcg acgccggtgt cggctacgaa cttctcggcc
     4321 agggccgggt cagtgacatg ttcgaggccg gtgccgccgg caccgtggcc gtcttcaatg
     4381 ccgccgagca cgccgatctc gccttcgacg gtcacgccca cggcgtgggc catctggacg
     4441 acctccctgg tgactttcac gttgtactca tagctgctgg gcgtcttgcc gtcctcgctg
     4501 agcgagccat ccatcatgac gctggtgaat ccctgctcga tagcactctt gcaggtggcc
     4561 gggctgttgc cgtggtcctg gtgcatggcg atcgggatct tggggtacag ttcgacggcg
     4621 gcgagcatca aatgtcgcag gtaagcgtcg ttggtgtact tgcgagctcc gcggctggcc
     4681 tggacgatca cggggctgtc tgtttccttg gccgcctcca tgattgcctg gatctgctcc
     4741 atgttgttga cgttcagtgc ggcgatgccg tagttgtttt cagccgcgtg gtccagcaag
     4801 atgcgcatcg gtacaagtgg catgtcgatt gatctcctca aacacgcttt gtggttcgtc
     4861 ccttcgctat cggccggcca gggcttggca gcgccccggt ccgggcaggc agacgcggaa
     4921 gtgtaccagc atccgcgagg aattgcacct agccggatcg agcgagcgga accggaggaa
     4981 gccggtcggg acggcggcct tggccgcagg accgatcgtc acccgaccct ctgcttgtaa
     5041 cctattgtcg agtaagatgt tatacgagaa catgtggtct tgccgtggga cgatcttttc
     5101 ggatgttggc gttcgaagca ggcagcagga gttgtgtttg gtaaaaggga aagtttctcc
     5161 ttgacacacc gtgttttgtt gaggaaaatg aggctgttga aggtcgggaa gctcgagtgg
     5221 ccgagcatgt ctaaaggctg atgaactcga gcagcactca catttcatgg gagagcggag
     5281 gggccggggg atgggaaggc aatcccggtc agaagaacat tgagcgttgg ccggcttcga
     5341 ccagtggcga tgtggctgtg gacttcgtga gcagtaatca gttcggccga agtcagcctt
     5401 tgcatatggt atcggtctga ggattggtgc cgaggcgcgc gccctcggca atcagcggtt
     5461 caagtatata gtgggttctc tttttggagg agcaaggcga tgaagatgtt actaggttca
     5521 attgcggtga tgctgtgcat tcccgcgttg gtgtgggccg atgcgccgcc ggggacaatc
     5581 gtctatatcg aggacgactt cctgccggac caggacatga acggctggac gacgtatggg
     5641 aacgcgacca tcgggaaccc ctcgggaaac ggattcagcc tgtgggggcc gtgctaccgc
     5701 agagctgacg agcaggatgg catcgccggg atccaggaag gcggcggtgg cggtcgcagc
     5761 ttcgggatgt acaagaggtt ttacgacgag tacattcctg agggcgaaaa gcacatccag
     5821 gcgtgggtcc gcatgcttag ctgcacctgg ggctgggaat tgacgccggg tgccctgaat
     5881 gtccggttgg gcaaggaccc gctcggcggg acggatccga atgccccgag cgtgatctgg
     5941 actgcgccgt actggggcga ctggaacaga ctcgagctga tccagccttg cgtcgagggc
     6001 tgggagacga tctttatcca ggtcgatggg ttcatgaacg gctactacca gattctggtg
     6061 gacacggtcg aggtctggca gactcctgag ccggcggcag ccgccctgct catgttgggc
     6121 ttgcccctgt tgcgtcgacg tcgctgaaga tcctattcgc ggcggcggta ggtgtgcggt
     6181 cggcaccgat cggcgtgctg ccggcccccg ccgaggggct gttccgcagg cgcgacgcgg
     6241 acaaacgcgc agcgcaatgg actactggaa gcttgcgagt cgtgttccgg ctcgcaggct
     6301 tccggtgcgc agacatggtg ccggtaatcg acaggacggc agccggggac tggatgttcg
     6361 accagttgaa tcggtccggg tgaagcattc cctgagtatc atcgggtatg tgggtccttg
     6421 cgtgcgccag ccgatgggag caaactggaa gcgtgaatcg gcgaagcaaa cgaaggcttg
     6481 aggacccacg gggcacaatc gcgacagggc tgccgaaaaa gcggatgaaa tggtggcagc
     6541 ggcttgctct gctgggcttt tcgttcatcc tgctcgtgct gatggaactg gcgttacggg
     6601 ttgtcggcta cggccccgat ccggatctga tggtgcgtcg agccgaagtc gagggcggac
     6661 gaatcatcgg cctcaatccg gtggcctgga agcgtttcgc gttgcgcccg actccccacc
     6721 gtcccggcga aggcgtcctc aaagtccacg atttccgcga cccgaagcca cccggaatct
     6781 ttcgggtttt ttttcttggc gagagcagcg tccagggggt gccctatgag cgaaacgcta
     6841 ccatgtgcgc atttcttgag gcgctgcttg cagcggcttg gcccgagatt cgcgtcgagg
     6901 tcatcaattg cggcgtcacg gcggtcaaca gctacagtct gcgagcctgg cttcccgagg
     6961 tgcttgaata cgagcccgac ctcctggtgg tgtatgcagg gcataacgag ttctacgggt
     7021 tttatgggcc ggggtcgttg cacggggtcg ggacaagccg gaaggctgtt ctcacccaca
     7081 tgtggattcg ccagttgcga ctcagccagg tcatccggga cgggcttgat acgttccgct
     7141 cgcctccgcc gccggagtct gcggcaacac tgatggaaat gatggcgaaa gaccggctga
     7201 tccgactagg aagtggcgtc tacagcgcgt gccgcgaaaa cttccgggcc aatttgaacg
     7261 acatggttca agcagcgcag ggagcaggcg ttccgatggt tctctgcggt ctggtcagca
     7321 acgagcgaga tctggtgccg atggtttctg ccaatcgtga gggtctctcg cgacagcaag
     7381 aagaccaatg gcgcagggac tttcaggaag gggccggtat ggtgtcggcc ggccgatggg
     7441 gggaggctct cccgcttctc gagcgagccg ctcaaatcga tgacacccat gcggagctgg
     7501 tttaccgcct ggcccagtgc cagcagcatc tgggacagcc gcacgaggcc agggccctct
     7561 atcgccgggc tcgagatctg gattgcctgc ggtttcgggc caccggcgaa ttcagtgagg
     7621 tgatccgcag cgtggccgga aagcgttgca tcttcgttga caccgccgcg gccttcgaag
     7681 ctgccagtcc cgacggactc atcggctgga acctgatgac cgatcacctt caccccacgg
     7741 tatacgggca ctacctgatc gcccggagta tctgccatgc gctcgcggcg gaatgtgagc
     7801 gtcttggtct gcgtgccata gagcccgatt cgcttccgca atttgaacgg gcggcggagg
     7861 tgctgggctg cgaccaactg accgagctga tcggatccct gcacatcctg aaactgatgc
     7921 aggcgtaccc gtttgcgggc acgccaaacg ccgcccgggc cgctgaactc ctgaccagta
     7981 ccgcgcgtga catggcatcg ctgccgcagg aaatcgttcc cgtgatcgat caatggatca
     8041 agggaggcag caaaggctcg ctgcacttcg cggtagcccg cctttacgag tcgagcggcc
     8101 aaaccgacaa agcaatgcct tatttgcgcc gcgccgagcg tacgacggag cctcacagcc
     8161 ctgaagcggt cgagatcaaa ctggagatgg cccgctttct cgccgcctcg acccggccgg
     8221 aagatgcgga acgggctgcc gaatacgcgc agcaggcctt gcagtatgtg gaggagtcgg
     8281 ctcgtgtgca tccggaggcg aaatcgcggt tggccgccgc gagcgcggag attcgacaga
     8341 tgattcgtta gtttctacaa tcgcctccgc atgaggttca tgggctgtcc gatggctcgg
     8401 attcaacgac atgacgggaa gttgtcgact ctccgggagt tgcttgcctt catgtgggga
     8461 cacaagcgat ggtggctgtt gcccgtggcg atcgtgctgc tgctcctggc gggtcttatc
     8521 gtttttgcgg agtcatccgc cttggcccca ctgatctaca cggtgttctg agggacgcgc
     8581 ttggcggtgc atatcctcgg catctcggct ttctaccatg acagtgcggc ctcattgctg
     8641 cgggacgggc ggattgttgc cgccgcccaa gaggaacgat tcacgcgcag gaagcatgac
     8701 gcctcttttc cccgcgaggc catccggtat tgtctgcggg aaggcggcat aggcggcgat
     8761 cagcttgact atgtggtatt ctatgacaag ccgttgctga aattcgatcg gctgctgcaa
     8821 acctattgct cgtttgctcc gcggggaatc cggtcgttcc ttaccgcgat gccggtctgg
     8881 ctgaagagca agctctggct gcgcaacctg attcgggagg aactggccgg ttgtggcgag
     8941 atcctcttct gtgaacacca tgggtcgcac gcggcgagcg cttttttccc cagccccttc
     9001 gaaagagcgg cgatactgac catcgacggc gtgggcgaat ggacgaccac ggcctggggc
     9061 gtgggggagg gcaaccgcat ccggatcgat gccgagatct gctttcccca ttcgctgggg
     9121 cttctgtatt cggcattcac ctattactgc ggattccgcg tcaacggcgg cgagtacaag
     9181 ctcatgggcc tggctcctta cggtgagccc cgatacgtcg atcttattct cgaacgggtg
     9241 atggagctga aggaggacgg ttctttcagg cttaaccttg actacttcaa ctactgcacc
     9301 ggcctgacga tgacgagccg tcggctgcac cgcctgtttg ggggcgagcc gcgacgacct
     9361 gaatccgaga ttacccagcg ggattgcgac atcgcaagga gcgtccaata cgttaccgag
     9421 gaagcgatgt tgcgcatggc tcgccacgtt cacgagcaga ccggatgccg ccatctctgc
     9481 ctggccgggg gagtagcgct caattgcgtc ggcaacgggc gcattctgcg cgagggaccc
     9541 ttcgatgaca tctggattca gccggcttcc ggcgacgccg gtggtgcgct gggtgcagcc
     9601 ttgtcggtct ggcaccaata cctcggccgc gagcgggaga cgggcggcgg ggcggacggg
     9661 atgcaaggcg cgcaactcgg tccgtgcttc gtgaccgacc agatcgagcg attcctggag
     9721 caggaacata ttcccgcgaa ccattattcg ttcagcgaac ttgctcaacg cgcagcggag
     9781 ctgatcgctt ccgggcacgt gctcggctgg tttgacggcc gtatggagtt tgggccccga
     9841 gccctcggca accgcagcat actggccgac gctcgaaaac cggagatgca ggccaccctg
     9901 aacctcaaga tcaagtttcg cgagtcgttt cgccccttcg ctccggccgt tttgctggag
     9961 cgcgtcaccg actacttcga actcgatcgt ccgagcccgt acatgttgct tgtggctcgc
    10021 gtccgcgagt cgcaccgacg tcttccttct gaaagcgaac gacgcgaagg gctggatcgc
    10081 agaaaggaga ttcgtagcga cgtgcccgcc attacccacg tggactgctc ggcaaggatt
    10141 cagacggttg atgtccggga taatgatcgc tttcaccggc tcctttgcgc ctttgaacac
    10201 ctgacgggat gcccgatggt catcaacacc agcttcaata ttcgcgggga gccgatcgtc
    10261 tgttcaccgt cggacgccta ccgttgtttc atgcgaacgc agatggacta ccttgttctc
    10321 ggggactacc tgttggccaa gggttgccag cctgagtcca agcggcaatc gattccttcc
    10381 ccggcggaga tcatcgactg accatgcgtg gctgcggtat ccatggtgaa ccttcccaga
    10441 gagcggagct acgtcgattc ggcgcagtct tgggattggc tctcataatt cttgcagtgg
    10501 tcttctgctg ccgacgacat tcgctgtacc tgccggccgc gatggccgtg gccggttccg
    10561 tgctgatggt atcaggcttg gcattcccct tggtgcttct acctgtccga cgcacgtggg
    10621 cgatgctggg ggcaggattg ggttggctca atactcgtgt tctgttgacg atcgtgtttt
    10681 acgcaatcgt aacgcctacg tcactgttgt tgcggcttgc acggcgagat ccgatgaatc
    10741 gtcgttggcg cgagcccggt acgagcagct actgggtcag gcgcgagccc aaaccttttg
    10801 agagcggtga catgcgcaga cagttctgaa tccgccttgg ctcccacggg gcttggacgt
    10861 ttgatcgggg cacgccgtgc ggttgtgtgg ccggcggggc ttgtaccatt cgtaatctgc
    10921 agatcaagct tgctgacgcc gccgcaggac gagaataacg gctgacgcca gcaaagcgag
    10981 gctgaagggt tcgggaaggc accggctttc cagatcaaca ttgtcgatac ccacaacccc
    11041 aagcttggga aaatgcacgt gggactcgaa cgccagctcc agaaacctgg gctggaaggg
    11101 gagttccagt cggatgtccg cgtgcttcca ctccggcgct cccgtgtcgg gataccgcca
    11161 ctcctcgaac acgagatgac gatgacaatc gaggccgtcg ggggcaggcg gctcgctgcc
    11221 gtcgccctgc caatggaggt agacgaggag gcccaccttt cgctgagcat cactaccgac
    11281 ggaatcaaga agataatcga actcgaggtc gatgagcttg cctgtcccgg ccggttccca
    11341 gcctggggat tgggttccgt ccattacttg cctgagcacg ccggccggct cattgtggtt
    11401 gccgttccag cgcaagatct ggccggccca cttttggccg ccggcggggt tgaccggaac
    11461 gtcgtcttgt ccctggttga acaagccaaa cgatgattgc gtccacggcg tccagccccc
    11521 cgtttcaaag tcgccgttgg caatctcgtt tgccaggcca agcgtaccgg ccactgcgga
    11581 ggccgccgac accaggtaca gcgcgaggca tacaagcttg cacatgatct ctctctccct
    11641 tgttgtacag gcaacgatca cataaccaca ggccctcata tgggccgacc ggcgcagcgt
    11701 ctcggaggaa cgttccgcgc cgaacgttga cctccgtgcc ttgctgccgg atcatccctg
    11761 acggttggtt ggtcccaggc ttatccccga ccacggagag tggttcccac gaagccgacc
    11821 ttgagagctt ctgcttcgtt ccagataaag gtattcttgc ccgatgctat tgccggtgaa
    11881 ttgcaacgtc aaggaatcgg ccgcaggagg ctcttcggcg attgccatgc ccgtcggcca
    11941 ggtgcccgag gcgagattct tgcccgtagg cctgtcggat cgcatccccg cttggtggct
    12001 ggacggcggt cgcttgatgg attaaggttt tctagagctc tcccctctgt cgggcccagg
    12061 cgatagccag ggggatgccc gccgtgagcg gcgtctgggg atcatagccg agcagttccc
    12121 gggccttgga gatatccgcg acatatcgca tgacttcgcc ggggcgggca ggttcgtaac
    12181 tgactttggg tgtttttccc agtgccaaag agatgatgtt caccgcgtcc accagggtgc
    12241 tgccttgccc ataggccacg ttgacggtct ggttaacgac gcggccggcg agcaggccgt
    12301 caatcccgcg gcggatgccg tcgatgcagt cgtcaatgta ggtgaagtcg aaaaccttct
    12361 ccttgccgta aacgatgatc ggttcatcgc gtgcaattcg tcgcatgaag agcggaagaa
    12421 cgcgctccag ccgctcgatg tcgttgtcgt agcggccgta gacgttgctg aagcgaaaaa
    12481 ccaggtaggg cagaccgtag cactcggcat aagagtaaat gaacgcctcc ccggagatct
    12541 tgctggccga gtaggggctc tcggcaacga cgaaatccgc ctctgcctcc tctgtgacgt
    12601 ggcggcggat gtccccgtac acttctcgtg aactcccaaa aatgagcggc aggcggtgct
    12661 tgcgcgcata gtcgagcacc ttgaagcaca tggtaacgtt ttccagggcc cgctcggggt
    12721 gttcgaccag ctcgaaaacc ttggcatacg cggccagatg gaccaccaga tcaaacggcc
    12781 ctgccgggta gtccttttcg cgagcggtgt tcaggtcgat cagacaggtc tcgatctcgt
    12841 ccgtccagga attgggacgg caatcgatgc ccacgacctg gtcgccgcgg gcctgaaggg
    12901 ccaacgccag atttgtcccg atctgtccgc tcgatccggt tatgagcacg cgcatgctga
    12961 atgaacctct ccggttttgc caaagtcgat cggcttgccg cttgccagcc tattaccgac
    13021 ccgaccgcgg gggagtatag cggcagggcc gcggcatgcc aaccggcagg gctcggacta
    13081 tgggcgggca agcttgacga ggttcttgtc ggcccatacg cttgccgccg ttgacccttc
    13141 ggtattcctc gacggtctgg tggatgagaa tgtagaatcg caggagtatt ccggtggagc
    13201 acctgtccca tctgctcgat tacgtggccg gcctcgagta cttcagatac ctcaacggcg
    13261 tcgattatgc ggtcatgacg gtatacgccg ccatcgtggt gggtatcggg gtatacctca
    13321 agcgccgagc ctcgcgcagc ctggagcact attttcttgc cggaagacaa ctgccctggt
    13381 gggccctcgg cgtgtccgga atgctcaact tcctcgacat gaccggcacg atgctgatcg
    13441 tctcctttct gtatatgctg gggcccagag gattgtacgt cgagttcaga ggcggcgcgg
    13501 ttctcatcct ggcctttatg atgctgtggg tcgggaaatg gcactatcgc tccaagtgca
    13561 tgaccggtgc ggagtggtac gtttaccggt tcggctccga caacatggcc aaggcatccc
    13621 gactgattgg agccatcggc ggcattgttc cgagtattgg catgctcgcc tacctgatgc
    13681 agggcgccgg cacgttcctg tcgatgtttc tgccgttcgg gcccaagacc tgtgcggtat
    13741 tgatggttgc cctgacggtg gcatacacct tgatgtcagg cttttacggc gtggtctata
    13801 ccgacctctt tcagtctttc atcatcctcg tggccgtcgt catcatctcg gtgctggccg
    13861 tcacccacgt ggtcggctac g
//
LOCUS       DAOTPW010000151        13867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4324_length_13867_cov_5.787648, whole genome shotgun sequence.
ACCESSION   DAOTPW010000151 DAOTPW010000000
VERSION     DAOTPW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13867
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4324_length_13867_cov_5.787648"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="P5316_13930"
     CDS             complement(<1..775)
                     /locus_tag="P5316_13930"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRR86103.1"
                     /translation="MHLRLLGKRPVVVWWVLTLLATCASTLSVKAQDSVSPAIASQPA
                     ADVASPATDEVDNPVLSPATVTPRADVASETHGVSPLPAIPLPFSETGEAAASPAAAQ
                     PDPAPSSNSSLASPEATDEPAKTETPAVPTSTPEPPPTPDHEEKHESVAGPHDKHETD
                     TPHETMGKHEAAAHGAHGEGAAGAHYSPPLWAVAPFVILLLCIAILPLIHRVEHWWHH
                     NKNKFIIAAALAIVTLLYYLLRSVGFHADPGLPSVKMVLQ"
     gene            1131..1553
                     /locus_tag="P5316_13935"
     CDS             1131..1553
                     /locus_tag="P5316_13935"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRR86104.1"
                     /translation="MQITRMTIGDHQFEVWLAKSNRQRELGLMRVKPEELSSFQEPTG
                     RGLSDIHRGMLFVFPFEQPLSFWMYNTITPLDIAYIRNDGRIVGIHTMAPLETRTYPS
                     GEPARFALEVKAGLFASIGVKVGDRVQIPESVLKDLPR"
     gene            1629..2879
                     /locus_tag="P5316_13940"
     CDS             1629..2879
                     /locus_tag="P5316_13940"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRR86105.1"
                     /translation="MSGSTTLTDLSVQPVPTASKPFRLLIVGSSEDLPQALVKLIERH
                     EADVVLAASLQDARRPKLLADVETVILCAHQESDARRAADRAAFLLADSLVSHHLMGL
                     AFSSGTYGVVPGCGDVFIRLPTDVSSQELSGWLSALRHVRPHLRRINNQVAAMQRLGR
                     RVNENLLEVDQELRLAGRLQRDFLPKVFPELADVRFAAMYRPATWVSGDIYDVRRLDE
                     RHIGFFLADAMGHGVAAGLMTMFIKEAIVGRRTREGEAPVRCPSKVLTSLNADLTAQQ
                     LPNCQFVTACYGQIDTDSHEVVLARAGHPHPIHVGSDGQAREVKTAGGLLGVFPEGDF
                     PSIKLTLQPGEKLVIYSDGLENIISGGHDAGGHSTFGPVFLQAIRLPGQECLDMLAQH
                     LDGAEGSLQPSDDQSCLVVERLCA"
     gene            2942..3616
                     /locus_tag="P5316_13945"
     CDS             2942..3616
                     /locus_tag="P5316_13945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR86106.1"
                     /translation="MAELQGLFLDFYGTVVGGDRLAVESACQAVVRDHHLPLTAAELA
                     VRWGRAYFRAIEAPDGDGFRNLVEIERDTLIETVSPLVGHIDPEPYVAILQDYLANPP
                     LCPEIREVLRHKRLPVCIVSNADNREIHKALERNDLRFDFIVTSESARSYKPDPTIFG
                     TALRLTGWRPERVIHIGDSLHSDVGGARAAGLKAVWVCREDRISDVGCEQPDQTWSDL
                     RPIINL"
     gene            complement(3936..4307)
                     /locus_tag="P5316_13950"
     CDS             complement(3936..4307)
                     /locus_tag="P5316_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86107.1"
                     /translation="MFELNGFFCPARDGASPAGEPPAFGDLGVDEALAPGGSAENGLT
                     VGLPVVTDGLDCPGEVPEGDDGRADFGAGLETEGAPLGVMLGRKAEPPPLIGGPTKVD
                     RFKSLDRPKLLLEDDPDRGLL"
     gene            complement(4413..4562)
                     /locus_tag="P5316_13955"
     CDS             complement(4413..4562)
                     /locus_tag="P5316_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86108.1"
                     /translation="MVELPGEALLSDWDDAPLLFETPVGALRPANDGIKVVGVWLDFD
                     PAEGA"
     gene            4597..5916
                     /locus_tag="P5316_13960"
     CDS             4597..5916
                     /locus_tag="P5316_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86109.1"
                     /translation="MPTGSTVGKSGAASGGSSGDRFFSGPSRSSSGFTGSVKKSTAGG
                     YFINPERSGRLNISVNRHSRDGDGRYFFRDERGRHDYCGCHRPRYECHHHRHSSYWFG
                     FGFFDSGPCYYPVYPVYPVYTWPVYEVVAAPTYVVQPAPVVAQAPVAVPGAVAYPGQA
                     APGVEVLTPGAYATVPPGQQPAEQPQEQAAQPQAQPQEQPAGPTQPQPPTASSSPDTA
                     QSGEMPVDEMDRLMKEGVEAFAAGDYEKAAQNFMRVSMAMPDNVDALLAYAVARFATG
                     DYTNSATAIRRAVRKVPEVVNSLFDLRDRYGKMSDFDGHVANLVRYVNEKKEDIDGWL
                     VLGFIQHFIGDRTHSKQTFEGIKQRSPADADIAEFFINAKSVEQLAKEAREAQDREQS
                     GTTQPSAQESSSQTLPSSVGTAGSQAPTPGPVQPAAAVSSGDFSDPF"
     gene            complement(6010..8061)
                     /locus_tag="P5316_13965"
     CDS             complement(6010..8061)
                     /locus_tag="P5316_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRR86110.1"
                     /translation="MAISLPTTEQDGTGLIKWDPWLRPYAEALRQRYARYRAGLDRLV
                     SAFGSLDAASRGHEYFGLNKGTHEGRPGVWYREWAPGATGLFLTGDFNGWNRQSHPLT
                     RDEFGVWSIFLPDDHYADHLTHGSRLKVHVHTPTDARDRIPAYIRRVTNEPHSADWAG
                     QYWTPPEPYVWKHVPPQARSPRIYEAHVGMATEEERIGTYQEFTRNVLPRIAEAGYNA
                     VQLMAIQEHPYYASFGYHVSNFFAPSSRFGTPEDLKELIDTAHGLGLLVVLDIVHSHS
                     VKNVNEGLNRFDGTDHQYFHAGPRGEHPAWDSLLFDYGKTEVLRFLLSNVRYWLEEFR
                     FDGLRFDGVTSMMYLHHGFRNFSSYDDYLIHDIDTDAVVYLQLANQVAHEVNPKAITI
                     AEDVSGMVGLARPLDEGGIGFDYRLAMGIPDYWIKLLKEYRDEQWNMGEICHTLTNRR
                     PAEKHIAYAESHDQALVGDKTIAFRLMDADMYWLMSKAVGSNLVIERGVALHKMIRLI
                     TFALGGEGYLNFMGNEFGHPEWIDFPREGNGFSYKYARRQWSLCDDPLLRYRDLAAFD
                     RELQALDERCDLLAASNTEIIHTDEAAKLIAFRRGSLVFVFNFHPTDSHPNYRIGVPE
                     PSDYKVVLNTDDFWFGGHGIVATGQIYPCQYQPAHGRNQSIQIYLPTRTAQVLATMPT
                     G"
     gene            complement(8132..11164)
                     /locus_tag="P5316_13970"
     CDS             complement(8132..11164)
                     /locus_tag="P5316_13970"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRR86111.1"
                     /translation="MGFRSPSIDYRPAPFWSWNDDLIEDELRWQIQQFKAAGFGGFFM
                     HSRVGLETEYLSDEWFAKIGACIDEARRIGMLAWLYDEDKWASGFAGGWLNRRFPETR
                     GVGLAATAIQKGELDKALAAPDTLAVFAIDKDERGTVCSSRRLKTGDVPTNGEMIWCY
                     YARPFANDNWFNGETYLDILNPQAVKHFLDITIDGYHKRFRNDFGRTVPGIFTDEPNF
                     YPWVGADLTAMPWTAGMADLFKRRFGYDLLDQLDALAFRRPGFQKVRHDYHRFLTERF
                     VESFAKPYGEFCDRLGLKLTGHWLWEDSLRSQVMHIGAAMPHYEYEQVPGIDHLRRNI
                     EDPLTLKQVASVAHQFGRDQVLCEIFGVSGQDFSFEDAKWIGDFHLALGVNYFCPHLT
                     LYSFTGDRKRDYPPTFSYHQPYWDRMSLINDYFTRAGWVTRQGEFQTKILVLHPIASA
                     WAFVSRFDDCPEVDFYHQELISLQSGLLGRHLDYDYGDEMILARHGRVEGRNLRVASH
                     GVYEVVLVPPSLTWTSETVTLLEELLAAGGRVVFVGQLPTAIDGEPRGDRWAPLHGHP
                     NARRVPNETQAVAEVLEEMGLREISVTGTDGREIESILCHHRRSGDKNIYFICNTSRQ
                     TAYETRITVNAGGSALCCDMVSGDMRPIPAHVSEGRLILTHRFEPVGSLAIMVDPQQS
                     LVGRMADHRETAREILQGDWSFARTHPNTLVLDTCRWAINDGPLSEPSPVWKVRRAAF
                     DAAGLQEYRGIQPWVLRQKGIVPGKTAKVEMRFAFKSEIDQPNASLVVELAEDFHIRL
                     NGHEIPADRSQWQWDKKFRKIPVGSQIHKGANDLLLTAVYRPGVEIEDIFIVGDFATR
                     QVGENSYVIVPEPDRLTIGDWGPQGYHFYSGNMVYRRSIRCARRLGQRTVLRLNQPAG
                     TLFEVRVNNQTAGLLGWQPWELDITEFLGSENNDLEIIVYGSLRNTFGPLHNRQYLEK
                     GNNWWIGPEAFADEANWTDRYVFTPYGLLGGAELITFE"
     gene            complement(11437..12030)
                     /locus_tag="P5316_13975"
     CDS             complement(11437..12030)
                     /locus_tag="P5316_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRR86112.1"
                     /translation="MDIQQRITEIMKREAEAILAVRVTPEFEKAVLVLRDCKGKVLTT
                     GMGKAGHIAHKFAATLCSTGTPAAFLHPGEAAHGDLGLVGQDDCIVALSTSGKTNEII
                     EMLELGRHLGLATIIGITSHPDSRLRDLSDVILDMGIIKEACPLGLTPSASIAVMLAI
                     TDALALTLMELKGITRHDYGLRHHGGYLGRKARTDNV"
     gene            complement(12179..12904)
                     /locus_tag="P5316_13980"
     CDS             complement(12179..12904)
                     /locus_tag="P5316_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR86113.1"
                     /translation="MVEPLLQIAGLTYAYPDGTQALRGVDLAVSAGEKVGLVGQNGSG
                     KSTLLMCISGLYTGQGCIHCEGSELTGSRLRETRRHVGLVFQSPDDQLFMPTLADDLA
                     FGPVNLGLDAHEVGRRVEEVAAQMGLSELLDRPPHHLSMGQKRNAAIASVLVMRPALL
                     LMDEPSSNLDPRSRRRLIEVLKSLHATMLIASHDLALVGTVCSRVALIDEGRIVADGP
                     AAEILGNGELMDRHGLEIWRPAK"
     gene            complement(12897..13673)
                     /gene="cbiQ"
                     /locus_tag="P5316_13985"
     CDS             complement(12897..13673)
                     /gene="cbiQ"
                     /locus_tag="P5316_13985"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HRR86114.1"
                     /translation="MHHHYIDHFAYGDSPIHRLDPRAKILAVVAYSAALISLSRYQLP
                     SPGYLILPVVLLWWSGTPLWFVVKHTLMVSPFIVCLVAFSPVFDRTQVPSPFGPGTIT
                     GGWLTAATVLIRFVLGMFSLIALASTTRFPELLKGLEGLGVPKVLVTQLRFLYRYLFL
                     LVDQAMHLRQARAARDAGLGPMKWRWQSAAGLVGILFIRTLEQAERTHLAMIARGYDG
                     AIRLLRGLRWKPHDTLFLTGTAIYLFLLRWGGHLGEWLRG"
BASE COUNT         2734 a         4233 c         4197 g         2703 t
ORIGIN      
        1 gctgcaagac catcttgacc gagggcaagc ccgggtcggc atggaaaccc acgcttcgga
       61 gcaggtagta gagcagggtt acgatggcca atgcggcggc gatgatgaac ttgttcttgt
      121 tgtggtgcca ccaatgttcg acacggtgga tcagcggcag aatggcaatg cacaacaaca
      181 gaatcacaaa cggagccacc gcccaaagcg gaggcgagta gtgcgcgcct gccgcgcctt
      241 cgccgtgcgc accgtgtgct gccgcctcat gcttgcccat cgtctcgtgc ggagtatcgg
      301 tttcgtgctt gtcatgcggc ccggctaccg actcgtgctt ctcttcatgg tccggtgttg
      361 ggggcggctc cggtgtcgag gtgggaacgg caggtgtttc tgtcttagcc ggctcgtccg
      421 tcgcttcggg ggaagccaac gaggagttgg atgacggagc agggtcaggc tgtgcggccg
      481 caggcgacgc cgccgcttcg ccggtctcgg agaatggaag cggaatcgcc ggaagcggcg
      541 acacaccatg tgtctcagag gccacatcag ccctcggcgt caccgttgct gggctgagaa
      601 ccggattgtc cacctcatct gtcgcgggcg acgccacgtc ggccgcaggt tggctcgcga
      661 ttgcggggga aaccgaatcc tgtgccttta cgctcagagt tgaggcgcaa gttgccaaca
      721 gcgtcagaac ccaccagacg actacgggac gtttacctag caggcgcagg tgcatgaacc
      781 agatctccgt ttgtcagggc tgggtgtcgg ccacgactcc atcatcccca gataccgtga
      841 cattatcggc agattgcggt ggtgggactc catgtctata ctggccttga gctgagggac
      901 agccaaccgt caccaagtgt tgcccgttga gagcgacggc ctcatgacgc gaccggatga
      961 ctcgtgggcg aggatgtgaa tccgtgtttg cttacgaaca tgtcgggcat ctcttttatg
     1021 ggcgcaggcg gaaaaccgtc ttgctggtta tccttctatc cttttgttcc ggttgcaccg
     1081 agaatccggc cgctgatacg ccgggcgcag cccgaaatga cttgagcgcc atgcagatta
     1141 ctcgcatgac tattggcgat caccagttcg aggtctggct tgcgaagagc aatcgacagc
     1201 gagaactcgg tctgatgcgg gtgaagcctg aagagctgtc ctcatttcag gagccgaccg
     1261 gccggggctt atcggacatc caccggggca tgttgttcgt ctttccgttt gagcaaccgc
     1321 tgtccttctg gatgtacaac accatcacac ccctggacat cgcgtacata cggaacgacg
     1381 gccgaatcgt gggtattcac accatggccc cacttgaaac gcgcacatat ccttccgggg
     1441 agcccgcccg cttcgctctt gaagtcaaag ctgggttatt tgccagcatt ggcgtgaagg
     1501 tgggcgaccg tgttcaaatc cctgagtcgg ttctcaagga cctgcccaga taggtcgaag
     1561 ctgattctga gcaagtaccg gtgacggcgt cgaatctccg gcgggaccgg gggccagcag
     1621 cacacctcat gtcgggatca acgactctta cagacttatc ggtacagccc gttccgacgg
     1681 catcgaagcc attccggctg ctgatcgtgg gttcctccga agacctgcca caggctctgg
     1741 ttaagctcat cgagaggcat gaggctgatg tcgtccttgc cgcatcgctg caggatgctc
     1801 gccggccgaa actcctcgcc gacgtcgaga ccgtcattct ctgcgcgcat caggaatcag
     1861 acgcccgccg agccgccgac cgggcggcat tcttgctggc cgactccctg gtaagtcatc
     1921 atctcatggg actggcattc tcgtcgggca catacggcgt ggtgcccggt tgcggcgatg
     1981 tgttcatccg gcttccgacg gacgtatcct cacaagaact gagcggctgg ctgtcagcgt
     2041 tgaggcatgt cagaccgcac ctccgccgca tcaacaatca ggtcgcggcg atgcagcggc
     2101 tgggacgtcg cgtcaatgag aaccttctcg aggtggatca ggagcttcgc ctcgccggcc
     2161 gattgcagcg ggacttcctg cccaaggtct ttcctgaatt ggcggacgtt cgcttcgcgg
     2221 ccatgtatcg ccccgccacc tgggtttccg gtgatatcta tgatgtccgc cggttggacg
     2281 aacggcacat cggctttttc ctggccgacg ccatggggca tggtgtggct gcgggtctta
     2341 tgacgatgtt catcaaagag gcaattgtcg gtcggagaac tcgcgaaggt gaagcgccgg
     2401 tcagatgtcc cagtaaggtt ctcacctcac tcaacgccga cctgaccgcg caacagttgc
     2461 ccaactgcca gtttgtcacc gcctgctacg gccagattga caccgattct catgaggtgg
     2521 ttcttgctcg tgccggccat cctcacccca tacacgtcgg atctgacggc caagctcgcg
     2581 aagtgaaaac ggcgggcggg ttgctcggcg tctttcccga gggcgacttc cccagcataa
     2641 agctgacact gcaacccggc gagaaactcg ttatctacag cgacggcctc gaaaacatca
     2701 tctcgggcgg tcacgacgcc gggggacact cgacgttcgg cccggttttc ctgcaggcca
     2761 tcagactgcc cggccaggaa tgcctcgata tgctcgccca gcacctcgac ggtgctgagg
     2821 gcagtcttca gccatcagac gatcaatctt gcctggttgt ggaacgactc tgcgcgtgat
     2881 tcgctgccag cccgggtccc tttcgttata atgccgttga gaaccagccg gaaaggatcg
     2941 catggcggag ttgcagggac tgtttctgga tttctacggc accgttgtcg gcggcgatcg
     3001 gctggcggtc gaatcagcct gccaggcggt ggtgcgcgat catcacctgc cgttgactgc
     3061 ggccgaactg gctgttcggt ggggtcgtgc ctacttccgg gccatcgagg cgcctgacgg
     3121 cgacggattc cgcaatctgg ttgaaatcga gcgagatacc cttatcgaga ccgtctcgcc
     3181 tctggtcgga catatcgatc ccgagccata cgtggccatc ctccaggatt acctggccaa
     3241 tccgccgctg tgcccggaaa tccgcgaggt tctgagacat aagaggctgc cagtgtgtat
     3301 cgtgagcaat gcggacaacc gagaaatcca taaagcatta gagcgtaacg acttaagatt
     3361 tgacttcatt gtaacgtcgg aatccgcccg cagctacaaa ccggatccaa ccatttttgg
     3421 gacggcactt cgacttaccg gttggcgacc ggagcgtgtc atccatatag gagactcgct
     3481 tcacagtgat gtgggcgggg ctcgagcggc tggtctaaaa gcggtgtggg tttgccgcga
     3541 agaccggatc agcgacgtcg gctgtgagca gccggaccag acgtggtcgg acctgcgtcc
     3601 gatcatcaac ttatgaggct tgttttcaca gcccgggaac catgaaatgt ggcgaaaaac
     3661 ggcagccaac cagcccctcg gggggatgat aaaggctggc aggaggctag agccatgtgt
     3721 ccgatcaatc gcgtgaacgc tttgtgggca atcgtttgtg gtgccctctg cctggccttg
     3781 acgaccgccg aaacgacggc ggcagacatg agcggccgag gaagcacaaa gcgatcgtat
     3841 tcttctgggt tttcgggtcg gtcgggcgcg tcgggtcact cgagcttctc aggcggctca
     3901 ggcttttcgg gtcgctccag cggcttctcc aaatcctaca gcagtccgcg atccggatcg
     3961 tcttcgagca gcagtttcgg tcgatcgagc gatttgaatc gatcgacctt cgtcggacca
     4021 cctatcagcg gaggcggttc agcctttcgc cccaacatga cgcccaaggg cgctccttcg
     4081 gtttcgagcc cggccccgaa gtctgctctg ccatcatcgc cttcaggcac ttcgccgggg
     4141 cagtcgagcc cgtccgtgac gacaggcaaa ccgacggtca gcccgttttc ggcactgcca
     4201 ccaggagcga gagcctcgtc gacacccaag tctccgaacg ccggaggttc gcccgccggt
     4261 gaggccccgt ccctggccgg acaaaaaaaa ccattcagct caaacacgac ggtcacaagt
     4321 ccgaaggcac cgttcacacc ttctgtcggt cagaccggcg gggtaaaggt gccttcccag
     4381 gtcagttcag gcaactcgaa atccggcggc gttcaagctc cttccgcggg gtcgaaatca
     4441 agccagacgc ccacgacttt tataccgtcg ttagccggtc gcaaggcccc cacaggtgtt
     4501 tcgaacaaca gcggtgcatc gtcccagtcg ctcaagagtg cttcgcccgg tagctcaacc
     4561 atcgtcaggc cgccctcggg gacgggcgga acgaaggtgc cgaccggctc tacggtcggc
     4621 aagtccggcg ctgcttcggg gggttcgtcg ggtgaccgat tcttctccgg cccaagccgg
     4681 tcgagcagcg gattcaccgg ctcggtcaag aaaagcaccg ccggcggcta cttcatcaat
     4741 ccggagcgat cgggcaggct caatatcagt gtgaaccgcc atagccgcga cggggacggc
     4801 cgctatttct tcagggatga acgcggccgg cacgactatt gtggctgtca tcgaccccgt
     4861 tatgagtgtc accaccacag gcattcgagt tactggttcg gatttggttt cttcgactcc
     4921 ggtccctgct attaccccgt ctatccggtg tacccggttt acacttggcc ggtatacgag
     4981 gtggtagccg cgccaacata cgtcgttcag ccggcaccgg ttgttgccca ggctccagtg
     5041 gccgtgcctg gggcggtggc ttatccgggg caggccgccc caggcgttga agtactcacg
     5101 ccaggcgcct acgcgaccgt cccgccgggg caacaacccg ccgaacagcc tcaggaacag
     5161 gctgctcagc cgcaggcaca gccgcaggaa cagcccgccg gaccgactca gcctcagccc
     5221 cctactgcat cgagctcgcc tgacaccgcg cagtccggcg agatgcccgt tgacgagatg
     5281 gaccgactca tgaaggaggg cgttgaagcc ttcgcggcag gagactacga gaaggctgcc
     5341 caaaacttta tgagagtctc gatggccatg ccggacaatg tcgacgcatt gctggcatac
     5401 gcggtcgctc gtttcgctac cggcgactat acaaactccg cgaccgcgat ccggcgggcg
     5461 gtccgcaagg ttcccgaggt ggtcaattcg ctgttcgacc ttcgcgaccg ttatggcaag
     5521 atgtccgact ttgacggaca cgtggccaac ttggtccggt acgtcaacga gaagaaagag
     5581 gatattgacg gctggctggt gctggggttc atccagcact tcatcggcga ccggacgcat
     5641 tccaagcaga cctttgaggg cataaagcaa cgttcgcccg cggacgcgga catcgccgag
     5701 ttcttcatca acgccaagtc cgtcgagcag cttgccaagg aggcccgtga agctcaggac
     5761 agagagcagt cgggcacgac gcagccgtcg gcgcaggaaa gctcaagcca gacgctgccg
     5821 agttccgtcg gaactgccgg ctcccaggcc ccgacgcctg ggcccgtcca gcccgccgct
     5881 gcggtctcca gcggcgactt ctccgatccg ttctgattct gaggcctgcc tgccaccgtc
     5941 cggccggcaa tgaggcgact cacggtgtgg gaggcacact cgaccttgac aaccggccga
     6001 cagcttcttt cagccggttg gcattgtcgc gaggacctgt gccgttcggg tcggaaggta
     6061 gatctgaatg ctttggttcc gaccatgagc cggctggtac tgacaggggt agatctggcc
     6121 cgtggcaacg attccgtggc cgccgaacca gaagtcatcc gtgttgagga cgactttgta
     6181 gtcgcttggc tcgggcaccc cgatccgata gttgggatgc gagtcggtcg gatggaaatt
     6241 gaagacgaac accaacgaac cgcgccgaaa ggcgatcagc ttggcggcct cgtcggtgtg
     6301 aataatctcg gtgttggaag cggcgagcaa gtcacaccgc tcatcaagag cctgaagctc
     6361 gcggtcgaac gccgccaagt cacgataccg cagtaacgga tcgtcgcaca gggaccactg
     6421 tcgccgggcg tacttgtagg aaaacccgtt gccctctcgg gggaaatcga tccactccgg
     6481 atgcccgaac tcgttgccca tgaaattgag atacccctcg ccgccgaggg cgaatgtgat
     6541 caaacgaatc atcttgtgta acgccacgcc gcgctcgatg accaggttcg acccgacggc
     6601 cttgctcatc agccaataca tgtcggcgtc catcagccgg aatgcgatgg tcttgtctcc
     6661 gaccagcgcc tggtcatggc tctccgcgta agcgatgtgc ttctcggccg gtcggcggtt
     6721 ggtcaaggta tggcagatct cccccatatt ccactgttca tcgcggtatt ctttgagaag
     6781 cttgatccag tagtcgggga tccccatggc cagccggtag tcgaacccga tgcctccttc
     6841 atcaaggggc cgggcaagtc cgaccatgcc cgacacgtcc tccgcgatcg tgatagcctt
     6901 tggattcacc tcgtgagcga cctggttggc cagttgcagg tagacgaccg cgtcggtgtc
     6961 gatgtcgtgg atcaggtagt cgtcatatga agagaagttc ctgaaaccat ggtgcaggta
     7021 catcatgctg gtcacgccgt cgaatcgcag gccgtcaaac cggaattcct ccagccagta
     7081 ccgcacgttg ctgagcagaa aacgtagaac ttccgtcttg ccgtaatcga acaacagcga
     7141 atcccatgcc gggtgttcac ctcttgggcc ggcatgaaag tactgatgat ccgtcccgtc
     7201 gaatcggttg agcccctcgt tgacgttctt gacggaatgg ctgtgcacga tgtcaaggac
     7261 gaccagcagt cccagcccat gagccgtgtc aatcagttcc ttgaggtcct ccggagtccc
     7321 gaaccgcgac gaaggagcga agaaattgct cacgtggtag ccgaacgagg cataatatgg
     7381 atgttcctga atggccatca gttgaacggc gttgtacccg gcctccgcaa ttcgtggcag
     7441 aacgttccgt gtgaattcct gataggtgcc gatccgctcc tcctctgtgg ccataccgac
     7501 atgagcctcg tagatgcgag ggctgcgagc ctgcgggggc acgtgcttcc agacataggg
     7561 ttcgggtgga gtccagtact gcccggccca atctgcggag tgaggctcat ttgtcacgcg
     7621 acggatgtag gcagggatgc ggtctctggc gtccgtcggg gtgtgcacgt gcaccttaag
     7681 cctgctgccg tgtgtgaggt gatcggcgta gtgatcgtcc ggcaggaaga tgctccagac
     7741 gccgaattcg tccctggtca gggggtgtga ttggcggttc cagccgttga aatcgccggt
     7801 gaggaacaaa cccgttgctc cgggcgccca ttcgcggtac cacacgcccg gtcggccttc
     7861 gtgggtgccc ttgttcaggc cgaaatactc gtgcccacgg ctcgcggcat ccagagagcc
     7921 gaacgcggat acaagccggt ccagaccggc ccggtagcgc gcgtatctct gtcgcagggc
     7981 ctcggcgtag ggcctgagcc atggatccca tttgatcagt ccggtcccgt cctgttctgt
     8041 tgtcggcaaa gagatcgcca ttccgaaatc acccccaggc aaccaccgcc ccgtctttgc
     8101 tcctgactca ataggatacg tgctgacccg atcactcgaa agtgatcagc tcagcgccgc
     8161 cgagcaaccc gtaaggagtg aacacatacc tgtcggtcca gttggcctcg tcggcgaacg
     8221 cctccggccc gatccaccag ttgttgccct tttcgagata ctgcctgttg tgcagcggcc
     8281 cgaaggtgtt ccgaagactg ccgtacacaa tgatctcgag atcgttgttc tcgctaccga
     8341 ggaactcggt gatatccagc tcccatggtt gccagccgag cagaccggcc gtctggttgt
     8401 tgacgcgaac ctcgaagagc gtgccggcgg gctggttgag tcgcaacacc gtccgttgcc
     8461 caagccggcg ggcacatcgg atcgaccggc ggtaaaccat gttgccagaa tagaaatggt
     8521 aaccctgcgg gccccagtcg ccgatcgtca gcctgtcggg ctcgggtacg atgacgtagc
     8581 tgttctcgcc aacctgccgg gtggcaaaat caccgacgat aaagatgtcc tcaatctcga
     8641 cgcctggtct gtaaacggcg gtcagcagca ggtcgtttgc ccccttatga atctgcgagc
     8701 cgacgggtat cttgcggaac ttcttgtccc actgccactg tgaacggtcc gccgggatct
     8761 cgtggccgtt gagccggatg tggaagtcct cggccagctc aaccacaagg gaggcgttcg
     8821 gctgatcgat ttcagacttg aacgcgaacc gcatttcgac cttcgccgtc ttgcccggaa
     8881 cgattccctt ctgtctcagg acccacggct gaatgccccg gtactcctgg agcccggcgg
     8941 cgtcgaaagc cgcacggcgg accttccaaa ccggcgaggg ctcgctgagt ggcccgtcat
     9001 taatggccca acggcaagtg tccagcacaa gcgtgttggg atgggtgcgt gcaaaagacc
     9061 agtccccttg caggatttcg cgggcagtct cccggtgatc ggccatgcgg ccgacgaggg
     9121 actgttgggg gtccaccatg attgccaggc tgccgaccgg ctcgaatcga tgggtgagaa
     9181 tcaaccggcc ttcgctgacg tgcgcgggga tcggccgcat gtcacccgag accatgtcgc
     9241 aacacagggc agagccgccg gcattcaccg tgatcctcgt ctcatatgcc gtctggcgag
     9301 acgtgttgca gatgaagtag atgttcttgt cacctgaccg ccgatgatgg cagaggatcg
     9361 actcgatttc acgaccgtcc gtgccggtga cgctgatttc ccgaagcccc atctcctcaa
     9421 gaacctcggc cacagcctgg gtttcgttcg gcacccggcg ggcattcggg tgaccgtgca
     9481 gcggggccca gcgatcgccc cgcggctcgc cgtcgatcgc tgtcggcaac tgtccgacga
     9541 aaaccacgcg acccccggcg gcaagcaact cctccaaaag agtgacggtt tcggaagtcc
     9601 aggtcaacga gggcggcacg aggacgactt cgtagacgcc gtgcgatgcg actcgcaggt
     9661 tacgtccttc gacgcgtcca tgtcgggcga gaatcatttc atcgccgtag tcataatcca
     9721 gatgccggcc cagcagtccg ctctgcaacg aaatcaactc ctggtggtag aaatcgacct
     9781 cggggcaatc gtcgaatcgc gatacgaagg cccatgcgct ggcgatcgga tggagcacca
     9841 gtatcttagt ctgaaactcg ccttggcgcg tgacccagcc cgcgcgagtg aagtagtcgt
     9901 tgatgaggct catgcggtcc cagtagggtt ggtgatagct gaaggtcggc gggtagtcgc
     9961 gcttgcggtc gccggtgaag ctgtacagag tcagatgcgg gcagaagtag ttgacgccca
    10021 gtgccagatg gaagtcgcca atccacttgg cgtcctcgaa actgaaatcc tggcctgaaa
    10081 cgccgaagat ctcgcaaagc acctggtccc gtccgaactg gtgagcaacg ctggccactt
    10141 gcttgagcgt gagcggatcc tcgatattgc ggcgaaggtg atcgatcccc ggcacctgct
    10201 cgtactcgta gtggggcatg gccgccccga tgtgcatgac ctgcgatcgc agtgagtcct
    10261 cccacaacca atgcccggtg agcttcaggc caagccggtc gcagaattca ccatagggtt
    10321 tggcaaagga ctccacaaac ctctcggtaa ggaaacgatg atagtcgtgc cggactttct
    10381 gaaaccccgg gcggcgaaaa gccaaggcat cgagctgatc cagaaggtcg taaccgaatc
    10441 gtctcttgaa aagatcggcc attcccgcgg tccagggcat ggccgtcagg tcagccccga
    10501 cccacggata gaagttgggc tcgtcggtga agatgcccgg aacggttcgg ccgaaatcat
    10561 ttcggaaccg tttatggtag ccgtcgatgg taatatcgag gaagtgcttt acggcctgcg
    10621 ggttcaggat gtcaaggtaa gtctctccgt tgaaccaatt gtcattggcg aatggtcggg
    10681 catagtagca ccagatcatc tcgccgttgg tgggtacatc gccggttttg agtcggcggg
    10741 aggagcagac cgtcccccgt tcatccttgt cgatggcaaa tacggcgaga gtgtcgggcg
    10801 ccgccaaggc tttgtccaac tcgcctttct ggatggccgt ggccgccagt ccgacgccgc
    10861 gcgtctcagg aaacctgcga ttgagccagc cgcccgcgaa gccgctcgcc cacttgtcct
    10921 catcatacaa ccatgccagc atgccgattc ggcgggcctc atcgatgcaa gcaccgatct
    10981 tggcgaacca ttcgtcggac aggtactcgg tttccaggcc gacacgcgag tgcatgaaga
    11041 atccgccgaa gccggcggcc ttgaattgtt gaatctgcca tcgcaactca tcttcgatca
    11101 aatcgtcgtt ccaggaccag aatggagcag gacgataatc aatcgacggc gaacggaatc
    11161 ccataaggtc gcactccctg gtgacgggcg tacacgaaac ggacaatgac gcggcaagaa
    11221 caagcggaag ccacagcttc atacgtcgca cctctttgcc ttgttttcgg gtaccgcaca
    11281 accgcggagt tgtaccgcgc agacgagtca ttgaccagtt gcccagctcg cggagctcag
    11341 cagacgtcaa acgtgaggct caaggattcc cgagatccga acctgccgaa gtcaatcaca
    11401 agagccggaa gctcatggcc gatcgctgac cgccgctcac acattatccg tccgcgcctt
    11461 gcggccgaga tagccgccgt ggtgacgcaa gccgtagtcg tgcctggtga tacccttgag
    11521 ttccatgagc gtcagggcca aggcatcggt gatggccagc atgacggcga tgctggccga
    11581 gggggtcagt ccgagcggac aggcctcctt gatgatgccc atgtccagaa tgacgtcgct
    11641 caaatcgcgc agtcgcgagt ccgggtgcga ggtgatgccg atgatcgtcg ccaggccgag
    11701 atgacggccg agttcgagca tctcaatgat ttcgttcgtt ttgccgctgg tggaaagggc
    11761 aacgatgcaa tcatcctggc cgaccagccc caggtcgccg tgggccgcct cgccgggatg
    11821 aaggaaagcg gcgggcgtac ccgtcgagca gagcgtggcc gcgaatttgt gtgcgatgtg
    11881 cccggccttg cccatgccgg tggtcagcac cttacccttg cagtcacgga ggacaagcac
    11941 ggccttttca aactccggcg taacccgcac ggccaggatg gcctcggcct cccgcttcat
    12001 gatctcggtg atacgttgtt ggatgtccat ggacaactgt cagctttcag cctggcgccg
    12061 ctggaacctg ctcccacgcc gcagagccgg ccagtctatc cggccggttg gaccgcagtg
    12121 caatcgggcc ccaggaacag accccgggcc cagcaagaat gcgacgacag tctaccgatc
    12181 atttcgccgg ccgccaaatc tctaggccgt gccggtccat cagctcgccg ttgcccagga
    12241 tctcggcggc cgggccgtcg gctacaattc gtccctcgtc aatcagcgcc acgcgcgagc
    12301 acaccgtgcc aaccagcgcc agatcgtgcg acgcgatgag catcgtcgca tggaggcttt
    12361 tcaacacttc gataagccgc cttcgcgaac gggggtcgag attgctcgag ggctcatcca
    12421 tcagcagcag agccgggcgc atcaccagga ccgacgcgat tgccgcatta cgtttctgac
    12481 ccatcgacag atggtgtggc ggacggtcga gaagctcgga cagccccatc tgcgccgcga
    12541 cctcttcgac gcgtcggccg acctcgtggg cgtcgagtcc cagattgacc ggaccgaacg
    12601 ccaagtcgtc ggccaacgtg ggcatgaaga gctgatcgtc gggactctgg aagaccagtc
    12661 cgacgtgcct ccgcgtctcg cgaagccggg agccggtcaa ctcggaaccc tcgcagtgaa
    12721 tacagccttg gccggtgtaa agcccgctga tgcacatgag cagcgtgctt ttgcccgatc
    12781 cgttctggcc caccagcccg accttctcgc cggcgctgac ggccaggtca accccgcgca
    12841 gtgcctgtgt accgtcgggg taggcgtagg tcaatccggc gatttgcagt agcggttcaa
    12901 ccacgcagcc attctcccag gtgcccgccc catcgcaaca ggaacagata aatggccgtt
    12961 cccgtaagaa agagcgtatc gtgcggtttc caccggagcc cccggagcag acgaattgct
    13021 ccgtcgtagc cacgggcgat catcgccaga tgcgttcgct cggcctgctc cagtgtgcgg
    13081 atgaacagga tgccgaccag ccccgcagcg ctttgccatc gccatttcat cggtccgagc
    13141 ccggcgtcgc gcgcggcccg ggcctgtcgc aggtgcatgg cttggtcgac cagcaggaac
    13201 aggtaccgat ataagaaacg caattgtgtc accagcacct tcggcacccc gaggccttcg
    13261 aggcccttca gcaattcggg gaagcgcgtg gtgctggcca gagcaatcag agaaaacatc
    13321 ccgagcacaa agcgaatcag gacggttgcg gctgtcagcc acccgccggt aatagttcct
    13381 ggcccgaagg gtgaaggtac ttgtgttctg tcgaaaaccg gtgaaaaggc taccaggcag
    13441 acgatgaacg gcgacaccat cagagtgtgc ttaacgacga accatagtgg cgtgccgctc
    13501 caccacagca gcacgaccgg caggatcaga taccccggcg agggcaactg gtagcggcta
    13561 agagatatga gcgccgcgct gtacgccacg accgccagga tcttcgcacg tggatcgagc
    13621 cgatggatcg gcgagtcacc gtaggcgaaa tgatcgatat agtgatggtg catggatcag
    13681 catctcgtgt cccccttggt aagggagtca gggggcgttc cgagggtcag cccacccgct
    13741 gttcaccccc cccccctttg tccccccttg ttaagggggg aaagaagacc gccgtgccac
    13801 ggttcaagtc ggggggttcg cactcgcggc cggcttcttg cgattccgat gcaatgatac
    13861 gccgatc
//
LOCUS       DAOTPW010000152        13817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4340_length_13817_cov_5.530083, whole genome shotgun sequence.
ACCESSION   DAOTPW010000152 DAOTPW010000000
VERSION     DAOTPW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_13817_cov_5.530083"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1125)
                     /locus_tag="P5316_13990"
     CDS             complement(52..1125)
                     /locus_tag="P5316_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HRR86115.1"
                     /translation="MAVLCHLADPKTYKAFDWDGVQDDQDRHYWLGLFESFTANIKKR
                     LIEDGLAGDGFAERWPLFEAEYAEGMRHIRAESERTGLQSTIDLCDFRQRMLNKYGWP
                     DPYLGVKTRENNMAARIYPKAVRDVDESLLPQRWELLFRMLFAGNMFDLGAPDTIDLY
                     NSGKLDFATILTEIRPRPWFIDHADAVRDRIMSPRRWKQALFFVDNAGTDIVLGVMPL
                     VREMARAGTRVVLAANSQPALNDITFDELNPLLDRLSQQDPVLADLRTSGIIATVANG
                     SNSPLIDLSRISDECNAAAADSDLLVLQGMGRGVESNWRQRFLCDVWRVALLKDRTVA
                     KWVGAKLFDPLCRFDEGERSHRD"
     gene            complement(1143..1568)
                     /locus_tag="P5316_13995"
     CDS             complement(1143..1568)
                     /locus_tag="P5316_13995"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRR86116.1"
                     /translation="MSQPVSSIELEIRVRYAECDPMGYLHHSKYFEYFEMGRTELLRA
                     AGFRYRDLEEKGVLFVVAKTACTFKRPARYDDLLKLEVRILRQTRARIDHEYRLLRDG
                     VILCEATTTLACVDRTGRPIPIPDEIAPPTDVRTGAKRD"
     gene            1641..2459
                     /locus_tag="P5316_14000"
     CDS             1641..2459
                     /locus_tag="P5316_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86117.1"
                     /translation="MSEPTSIAVPPVEDEGLRCIKCGYNLTGLPGSICPECGVTVDWE
                     AVRRKRDELRERIGTPWERWRWRLKPVAFVVTCLQVAFLPWRFARQVRDRPSLLSAGA
                     FLLVCMGLPILWFGGGILWTGPGDRDTLAWWMCGVICQVVFQTVVFGLLLPVDGIRGS
                     LRFWFAVTAYTSYPLLPEIWTGAPYIVDNESNLYPFNLLLRKSGSGCLWTSLLFYLWW
                     IGLVVVALIRLPRRRWWRIAIMIVAIPLMTYGSTYFGCQMAQGLDAAGKALGVR"
     gene            complement(2579..4126)
                     /gene="guaA"
                     /locus_tag="P5316_14005"
     CDS             complement(2579..4126)
                     /gene="guaA"
                     /locus_tag="P5316_14005"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRR86118.1"
                     /translation="MKETVAVLDFGSQYAQLIARRVRENRVYSTICPPTISPAQVREM
                     NVAGMIFSGGPASVYAPGAPGCDPALLRTGIPVLGVCYGMQISCQSLGAKVNAAPARE
                     YGRTRLEVIDSHDLLAHVQSPTTVWMSHGDMVQDLPADFVPLARTPNCPIAAVRHKTQ
                     PVYGVQFHPEVTHTPCGGQILRNFLYEICGCKGQWEMGNFIDEAVEAVRAKVKPDERV
                     ICGLSGGVDSSVVSALLHKAIGDRLVCIFVDNGLLRKNEVELVEATFRDHFQIDLRVV
                     RAADRFFARLAGVTDPQEKRKRIGHEFIEVFKAEAASIPNAKYLAQGTLYPDVIESGH
                     GISGTSANIKLHHNVGGLPAELGFELIEPLRDLFKDEVRTAGVHLGLPEEIIWRHPFP
                     GPGLAVRIVGEVTPQRVKLLQEVDEIVIDEIRSAGWYKSIGQAFAVLLPVSSVGVMGD
                     DRSYEGQQVAVIRFVETTDFMTADWVRIDYEVLERVSSRIINEVRGINRVVYDVSSKP
                     PSTIEWE"
     gene            complement(4136..6004)
                     /locus_tag="P5316_14010"
     CDS             complement(4136..6004)
                     /locus_tag="P5316_14010"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRR86119.1"
                     /translation="MKGPQFCIKHLRDTLSDNSLRHALDVLADATSASVVLFDNDGQV
                     AAGPVAGNDLIRRVLSTPQGRELVISTHRDTANRAAGKSPPGEPPSGTNALTAAPLER
                     FGIPICRSSQCAGMLTLGDRSRTPLSQEAISRIESAAGLPAGELRQAAASLKPWSPAE
                     ASAARNMAGLFAELLAELCLHEMSLSRRIEELTAVYNIASLLSGTMDLQEVLNRTART
                     VCEVMRVKACSIRLLDETSGNLVIRAVHNLSEEYLNKGAVTVEENPIDRAAMRGEVVY
                     IADAPTDPRTRYPQEARREGIVSGLVVGLIYRGKTVGVLRVYTGEPHVFTPFEAALLQ
                     AVASQAAAAIVNVRLMAETLEAERYSRQITYAGEVQRRMIPSQPPVCRQVEIGAVYRP
                     TYNVGGDFYDFIPLPNGNLGIAISDVSGKGVPASLVMASLRSALRVYAKFTYDIDHIM
                     AMLNKHVCAETKIGEFTTLFYGVLSPDGRRLTYCNAGHEPPMLLRNGQIQPLGTGGMV
                     LGIDETARFERELVSLQPGDVVLLYTDGAAEALNFSDEQYGRPRLGESLIRYADQPAQ
                     LIAQNILWDIRRFRGLADRIDDVTLVVLKIKDTAKQGPPGPDSTVQESRPPAAPSI"
     gene            7314..8288
                     /locus_tag="P5316_14015"
                     /pseudo
     CDS             7314..8288
                     /locus_tag="P5316_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
     gene            8298..8981
                     /locus_tag="P5316_14020"
     CDS             8298..8981
                     /locus_tag="P5316_14020"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR86120.1"
                     /translation="MPPLPDRAARTADIIEPPKPKEDKPKADGGGFVPLFDGKTLNGW
                     TIYTHEGKKVEPKDSSWSVPEDGVLHTTGDESKDYWISTDEKYGDCVLRMDFKVSQGA
                     NSGIFLKVPGHDRPAYTGFEVQIIDDVGRPADKHSTGSIYDLFAPVANVSRPLGQWNS
                     IEITCKGSLVTVVMNGLKIIDCDFSLMTEPMGKFDFPYAKMPAIGYIGMQTHGNAVSF
                     RNIRIKKLN"
     gene            9182..10429
                     /locus_tag="P5316_14025"
     CDS             9182..10429
                     /locus_tag="P5316_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR86121.1"
                     /translation="MIRRIEALNYRCLKYISQELDDFHVLVGPNASGKTTFLDVVAFL
                     GDLVSGGLEQAMGGRTQNFQDLVWRRSGDRIELAVELEIPPERRGEADRAGHDRVRYE
                     VAIGPDRETGALAILAEKVLLKQTPHRQSVQRFLFPVEPETPESLITGKIARSVKTVV
                     NKVPGGNDNFYAETNKGWDHAFKLGPRKSALGNLPDDESKFPVASWLKGVLKEGIQRL
                     VLNSAVMRKPSPPSLPSSFLPDGANLPWVIEGLRQQKADRHHEWVVHLQTALPDLTDV
                     KTIVREEDRHRYLVACYRDGLEVPSWMVSDGTLRLMALTLLAYLPRLRGVYLIEEPEN
                     GIHPRAVEAVFQSLSGVYDAQVLLATHSPVILSIADSSEVLCFSKTESGATDVVRGTE
                     HPKLRDWRGETNLGVLFAGGVLG"
     gene            complement(10498..10626)
                     /locus_tag="P5316_14030"
     CDS             complement(10498..10626)
                     /locus_tag="P5316_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86122.1"
                     /translation="MGIPRGEWTEEVVAVQQTPRISLDVGVNVRLHWSNRECLVPF"
     gene            10708..11061
                     /locus_tag="P5316_14035"
     CDS             10708..11061
                     /locus_tag="P5316_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86123.1"
                     /translation="MAGTGWSNRAGAVVIDPELEVWVWSDSPHVEDVLDWRGQALPLR
                     SWLADQGLLQEGRLKPAHPKRALERALRLVGKPPSSGIYGDLARRVGLDRCTDPAFLK
                     LRRLLTSWFPPSGSA"
     gene            11497..>13817
                     /locus_tag="P5316_14040"
     CDS             11497..>13817
                     /locus_tag="P5316_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRR86124.1"
                     /translation="MSTLDQLPPTYEPQSVEPEIRRIWAESGCFHADPTANANEPYTI
                     VIPPPNVTGALHGGHALNNTLQDILIRWRRMQGYNTLWMPGTDHAGIATQAVVEKRIF
                     ETERKTRHDLGREEMVRRIWEWKEQYGNRIIEQLKLMGCSCDWDRTRFTLDEVCARAV
                     RHTFFKLFKDGLIVRGKRLVNWDTHLQTAVADDEVYHETIQGHFWHFKYPVKDPRPGE
                     PTHVHIATTRPETMLGDTAVAVHPEPDKALDKTEAELREKLAEVPDKEKRGVQAAIDE
                     IVERRKTVLPLLLKLRDMARDGRRLILPLVNREIPLICDVWAKPELGSGCVKITPAHD
                     ANDYEVGRRQGLPMINVMTPDGRVARIIEPDGAVNPNSRKYEGLRFATDARKKVVADL
                     EGLGLVEKIEDRIVEIGHSDRSKTPIEPFLSDQWFVRMGDLPAEEAAKIEGLRGSSGL
                     AQLAMDAVCEKQHASAAERKACGKVHFFPERYAKTYLDWLSEKRDWCISRQLWWGHRI
                     PVYRGLQIADHEEAKRRDAKAQEWLKAGRMAQAFGPVHSTSAPPPPGSPWYFVGPAAL
                     SREIYVCVKDADDDEIVAELQRWGFVQDPDVLDTWFSSALWPHSTLGWPAPNPLLPEA
                     DKLLERYYPGSVLSTAREIITLWVARMVLTGRYNIGKVPFHDVYIHAVIQDGQGRPFK
                     KTLGNGFDPVDIIEVYGADALRFTMANLSTETQDIRMPMKKVKLDNGREVQSSERFEL
                     GRNFCNKIWNAARFAFMNLGTPPSPPSTEGGSA"
BASE COUNT         2617 a         4116 c         4372 g         2712 t
ORIGIN      
        1 tgcgatccag aaacatgcgc gcaagccgca cagatatctg gccgtaaggt tctaatcgcg
       61 atgtgagcgt tctccttcat cgaatcgaca cagcggatcg aagagcttag cgccgaccca
      121 cttggccacc gtgcggtcct tgagcagggc gacgcgccag acgtcgcaca ggaagcgctg
      181 ccgccaattg ctctccacgc cccggcccat cccttgaagc accagcaggt cgctgtccgc
      241 ggcggcggca ttgcactcgt cgctgatccg gctcaggtcg atcaacgggc tgttgctgcc
      301 gttggcgacg gttgcgatga ttccgctggt ccgcaaatcc gccaacaccg gatcctgttg
      361 tgacagacgg tcgagcaggg gattcagttc atcgaaggtg atatcgttga gagcgggctg
      421 tgagttggcc gccaggacga cgcgcgttcc cgcccgggcc atttcgcgta ccaggggcat
      481 gactcccagc acaatgtcgg ttcccgcgtt gtccacgaag aacagggcct gcttccagcg
      541 ccggggcgac atgatccggt cgcggacggc gtcggcatga tcgataaacc acggccgggg
      601 gcggatctcg gtgagaatcg ttgcgaaatc cagttttccg ctgttgtaga gatcgatcgt
      661 gtcgggggcg cccaggtcga acatgtttcc ggcaaacagc atgcggaaga gcaattccca
      721 gcgttgcggc aataggcttt cgtcgacatc ccgaacggcc ttcgggtata tgcgggcggc
      781 catgttgttc tcgcgagtct tgacgccgag ataggggtcg ggccagccgt atttgttcag
      841 catccgctgc cggaaatcgc acaagtcgat ggttgactga aggccggtcc gctccgactc
      901 agcccggatg tgccgcatgc cctcggcgta ttcggcctca aacagcggcc atcgttctgc
      961 aaaaccgtcg cccgcgaggc cgtcctctat gagccgcttt ttgatgttgg cggtgaagct
     1021 ctcgaagagg cccagccagt agtggcgatc ctggtcgtcc tgaacgccgt cccagtcaaa
     1081 cgccttgtac gtcttggggt cagccagatg acagagaacg gccatggtct gctcgcgttg
     1141 gattagtccc gcttggctcc ggtcctcaca tccgttggcg gggcgatttc gtcgggaatg
     1201 gggatcgggc gcccggtgcg atccacgcag gccaacgttg tggtcgcctc acagaggatg
     1261 acgccgtcgc gaaggagccg gtattcatgg tcgatccggg cccgcgtttg acggaggatg
     1321 cggacttcga gcttgagcag atcgtcgtaa cgggccggtc ttttgaaggt gcaggccgtc
     1381 ttggcgacca cgaacagcac gcccttttct tccagatcgc ggtaacgaaa cccggcggcg
     1441 cggagcaatt cggtccggcc catctcgaaa tactcgaagt acttcgagtg gtgcaggtag
     1501 cccatcgggt cgcactcggc ataacggacc cggatttcaa gctcgattga cgacaccggt
     1561 tgactcattc ggtctttcct tgctctggcg agcgaatcat ggtactcgtg atggcgtgcg
     1621 aatcaacagg agcaagtcca ttgtccgagc ctacatccat tgcggtgccg cctgtggagg
     1681 atgaaggcct gcgctgcatc aagtgcgggt acaacctcac cggcttgccc ggttcgatct
     1741 gcccggagtg cggcgtgacc gtcgattggg aggccgttcg ccgcaaacgt gacgagctgc
     1801 gcgagcgcat cggcacgccc tgggagcgat ggcgctggcg actcaaacct gtggctttcg
     1861 tggtcacgtg tctgcaagtg gcgttcctgc cgtggcgatt cgcccggcaa gtgcgcgacc
     1921 ggccgtccct tctatcggcg ggggcgtttc tgttggtgtg tatgggtctg ccgattctct
     1981 ggtttggggg cggtattctc tggactggtc ccggcgatcg tgacaccctt gcctggtgga
     2041 tgtgcggcgt gatctgccag gttgtctttc agaccgtcgt gttcggtctt ctgctgccgg
     2101 tggacggcat acgtggatcg ctgagattct ggtttgcggt caccgcctat accagttatc
     2161 cccttctccc tgaaatctgg accggggctc catacatcgt cgataatgag agcaacctgt
     2221 acccgttcaa tctccttttg cgcaagagcg gctcgggttg cctttggacc agcctgctgt
     2281 tctatctctg gtggatcggg ctggtggttg tggcccttat tcgcctgcct cgccgacgat
     2341 ggtggcgaat tgcgatcatg attgtggcaa tcccgctgat gacatacgga tcgacgtact
     2401 tcggatgcca gatggcccag gggcttgacg cggcgggcaa ggcactgggg gtgcgatgaa
     2461 gcgaaggatg aaatagaggg gatgaaggat gaatcagggg aggcacagga agatcggcga
     2521 gttgcctgcg acgttcagcc gtttcatcct tccgccttta tccctcatcc ttctgatctt
     2581 actcccactc aatcgtgctc ggcggtttgc tggagacgtc gtagaccaca cggttgatgc
     2641 cgcggacctc gttgatgatc cgggaagaga cgcgttcgag gacttcgtaa tcgatccgca
     2701 cccagtcggc ggtcatgaag tcggtggttt cgacgaatcg tatcacggcg acctgctggc
     2761 cttcatagct gcggtcgtcg cccatgacgc ccaccgaact gaccggcaga aggacggcga
     2821 aggcctggcc gatgcttttg taccagccgg ctgagcggat ctcgtcgatg acaatctcgt
     2881 ccacctcttg aagcagcttg acgcgctggg gtgtcacctc tccgacgatt cgcaccgcca
     2941 ggccgggccc tgggaaggga tgtcgccaga tgatctcctc gggcagtccg agatggaccc
     3001 cggccgtgcg gacctcgtct ttgaacaggt ctcgcagcgg ttcaatcagc tcgaagccca
     3061 actcggccgg caggcctccg acattgtggt gcaacttaat gttcgcggag gtccccgaaa
     3121 tgccgtgtcc cgattcgatc acgtcggggt aaagcgtacc ttgggccagg tacttggcgt
     3181 tggggattga ggccgcctcg gccttgaaaa cctcgatgaa ttcgtgaccg atgcgcttgc
     3241 gcttttcctg cggatcggtc acccctgcca gacgggcgaa gaaacgatcg gccgcccgca
     3301 ccacgcgcag atcgatctgg aagtggtcac gaaaggttgc ctcaaccagt tcaacctcgt
     3361 tcttgcgcaa caggccgttg tccacgaaga tgcagacgag gcgatcgccg atggccttgt
     3421 gcagcaaagc cgataccacg ctgctgtcca cgccgccgga gagtccgcag atgacgcgtt
     3481 cgtcgggctt gacctttgcg cggacggcct cgaccgcctc atcgatgaag ttgcccatct
     3541 cccattgacc cttgcagccg cagatctcgt agaggaagtt gcggagaatc tgcccgccgc
     3601 agggtgtgtg agtgacctct gggtgaaact ggacgccgta gacgggctgg gtcttatgcc
     3661 gaacggccgc gatggggcag ttgggcgtcc gggccagcgg gacaaagtcg gcaggcaggt
     3721 cctggaccat atcgccgtgg ctcatccaca ccgttgtggg cgactgaacg tgggcgagca
     3781 gatcatgaga gtcgataacc tccagccgcg tccggccgta ctctcgtgcc ggcgctgcgt
     3841 tgaccttggc ccccaggctc tggcaactga tctgcatccc gtaacagact cccagaacag
     3901 gtatgccggt ccgaagcagc gccgggtcgc acccaggcgc accgggcgca taaacactgg
     3961 ccggtccgcc ggagaagatc atgcccgcga cgttcatctc gcggacttgc gcggggctga
     4021 tcgtcggcgg gcagatcgtg ctgtagacac gattctcgcg gacccttcgt gcgatgagct
     4081 gggcgtactg gctcccgaaa tcgagtacgg caaccgtttc cttcatggtg gcggattata
     4141 tcgaaggcgc ggccggcggg cgagactcct gaacggtgga atcagggccc ggtggtccct
     4201 gctttgcagt gtccttgatc ttgagtacca caagcgtgac gtcgtcgatt cggtcggcca
     4261 ggccgcgaaa ccgacggatg tcccaaagta tgttctgagc aatcagttga gcgggctggt
     4321 cggcgtagcg aatgagagat tcgccgagcc ggggacgacc atactgttcg tcggagaaat
     4381 tcagcgcctc ggccgccccg tcggtgtaca gaagtacgac gtcgcccggc tgcaaggaga
     4441 cgagctcgcg ctcgaacctg gccgtttcgt ctattccaag gaccatcccg ccggtaccca
     4501 ggggctggat ctgaccgttg cggagcagca tcggcggttc gtggccggca ttgcagtagg
     4561 tcaatcgtcg gccgtcgggt gacagcacgc cgtaaaacag cgtcgtgaac tcgccgatct
     4621 tcgtttccgc gcacacgtgt ttgttgagca tcgccatgat gtgatcgatg tcataggtga
     4681 acttcgcgta gacccggagg gccgaccgca gcgacgccat gaccagcgag gccggcacgc
     4741 ccttgcccga tacatcggaa atggcaatac ccaggttgcc gttgggtagc gggatgaaat
     4801 cgtaaaagtc gccgccgaca ttgtaggtgg ggcggtagac ggcgccgatc tcaacctgtc
     4861 ggcagaccgg cggctgactc ggaatcatcc gccgctgcac ctcgccagcg taggtgatct
     4921 gccgcgaata gcgttcggct tcaagggtct cggccatcag ccggacattg acaatcgccg
     4981 ctgccgcctg ggatgccacg gcttggagca gggcggcttc gaacggcgtg aacacatgcg
     5041 gttccccggt gtaaacgcga agcaccccga cagtcttgcc gcggtagatc aaccctacga
     5101 ccaagccgct gacgatgccc tcgcggcgag cttcttgagg ataacgcgtc cgcggatcgg
     5161 tgggagcgtc ggcgatgtag accacctcgc cgcgcattgc cgcccggtcg atgggattct
     5221 cttcgacggt gaccgcgccc ttgttgaggt actcttccga aaggttatga actgcgcgga
     5281 tgaccagatt gccgctggtc tcgtccagca gacggatgct gcaggccttg acgcgcatca
     5341 cctcgcacac cgttcgggcc gtccggttga gaacttcctg caggtccatc gtgcctgaga
     5401 gaaggctcgc gatgttgtac accgcggtaa gctcttcgat tcgcctgctg aggctcatct
     5461 cgtggaggca cagttccgcc agcaactcgg caaacaggcc ggccatgttg cgggcggcgg
     5521 atgcttccgc agggctccag ggcttgagcg acgcggccgc ctgtcgcagc tctccggcag
     5581 gcaagccggc cgcggactcg atccgcgaga tggcctcttg cgataggggg gttcgagaac
     5641 ggtcgccgag tgtcagcatt cccgcgcact ggctgctgcg gcagatcgga atgccgaatc
     5701 tctccagagg agcggcagtc aaagcgttcg tacccgaggg gggctcgcct ggcggcgatt
     5761 ttccggcagc acggtttgca gtatcgcggt gcgtcgagat gacgagttcc cgaccctgag
     5821 gtgtgctcaa cacgcgacgg ataaggtcgt taccggctac cgggccggcg gccacctgcc
     5881 catcattgtc aaaaagtacg accgatgctg aagttgcatc tgccagaaca tccagggcat
     5941 ggcgcaacga gttgtcggac agggtatccc tgaggtgttt gatgcagaac tgtggcccct
     6001 tcattcaaca cttccccagg cgggttgcgg cgactcgcca atgagcatta taggcggttg
     6061 ttgggaatgg ggcaaaagcc gcatgccgac gcagcggatg tctacgggac ttccaggctc
     6121 acatcgccgt gaaccggtac ctgaaggcgg cccgcctttt tgcgggccgc aacccaagcg
     6181 cactgtgatc caaaatgatg cgcgcaagct gcgcggatgt ttagccgtaa ggttctcctg
     6241 gcggtcatcg actcctcaga tgaccgaacc tgactgaacg gggcctcccg acggggcgct
     6301 ggcaggggga tcgtcgctcg tcgccttctt tttttgtgcg gtatgtgcat atcggccgcg
     6361 cacgatctga caaccccaaa tacgcacaac ggcggatgcg ctgggattct tgaccctggc
     6421 gacatccgcc gttggcgtct ttatttggcc caggcttcgt cggcctgcac ggtcctggtg
     6481 ccggacccta tcggggtcct tctctgaaaa ctcggcgcct cgctcttgag gcatcgccgc
     6541 agttgtttct gcgagtcttg ttgttgggct cctgtccgtt cttacgtccg aacctcctgt
     6601 ttgacccagc accttggccg cggcctgcat atgctgttga tcttcgcggc tcgccgcgac
     6661 ccccctttcc cccggccggc gcacctacgg aggtgctcgt tctccttcgc cagagaaatc
     6721 atgaagccgc aatcgcaacg gggataagtt catccccgat cttgcggata ccttccaata
     6781 gaaagacccc ttcccctgct cgcgcacctt gtcgcggtca cgggactttt cctcaacagg
     6841 gtccagcgta ccgacctggg attcgccgat gatctccgga ataccgggca tcgctcgcac
     6901 ctcccattca gccgcgtatc cgcgggcatc atgcccgcgc tgggatgtct tcaatcgcct
     6961 cgtggccccc aggccatcta cggcaacgtg acgcttgata tggcctttcg ctgaaatcag
     7021 tataaaccaa aacgggccga atggcaaggc ttttttcgcg aatctctcag gcccgcgggg
     7081 acacacggcc gcggttgcgg gccccgatcc cgtgtggcat aatccgctcc accccgcccg
     7141 gtgaggcccg ggccgcggat gaggccaaag gtgttcttcc tgtttgattg aaaggagatc
     7201 ccatgtcgcg acggtgcaca ttgtgggttt gctcaatgtt gatcccgatg gcgtttgtcc
     7261 tcggttgcca ggggcaggga gccttttcat ccggcggggt aaatgccgcc gacatcgatc
     7321 cgacactacc ggttgttcca aatggcgcag tcggtcgcgc aatcacgtgg gagaatcgcg
     7381 ccggtgccgt tggtgccggc ggcgtgagcg aaagcaagct tggcaagggt cgcaagggct
     7441 ctccatgcat cccggaggtt aagaacggcg agacggtcgc gctgatggac attgacggct
     7501 gcggcgtcat caggcacatc tggatcacga ttccggaccg ctcgcccgag gcgatgcgaa
     7561 acatgatcct gcggatgtac tgggacggga gcgagatgcc cagcgtcgag gtgccgctcg
     7621 gcgatttctt cggagtggcc catgggcagt gccgggtgct gacgaccgcc tatgttactc
     7681 aggtcctggg ccgggggttc aacagctact tcgcgatgcc gttcggcacc catgcgagga
     7741 tcacggtgac caatgacatg cccgatggaa agaagatggg ggcgttcttc tatcaaatcg
     7801 actatgagtt gcgcgacaag ttgccgccga acaccggtcg tttccatgcc cagttccgcc
     7861 gcgaaaaccc gacggtgatg aagcgggact acgtgattct cgacaatgtc gaggggccgg
     7921 gctttttcct cggctgcgtc atcggcgtca gggccctcgg gccgcactgg tggggcgagg
     7981 gtgagatgaa gttctacatg gacggcgaca ccgacttccc gaccatctgc ggcacgggta
     8041 cggaggacta tttcgaggcc gcctggggca tgggggagta tcagacgccg tatgcgggtt
     8101 gcccgatgca tctgaccaac gacttcttca ggcacccgct ggtcagcctg taccgctttc
     8161 atgagaagga cccggtctat ttccgaaaaa gcttcaaggc cgtgattcag cagatcggct
     8221 ggaaccaggg gctcttcgag cgaagcgatg actggtgcag cgtcgcctac tggtaccagc
     8281 tcaagccggt caagcagatg ccgccgttgc cggatcgcgc ggctcggacc gccgatatca
     8341 tcgaacctcc caaacccaag gaggacaagc ccaaggccga tggcggcggg tttgtgccgc
     8401 tgttcgacgg caagacgctg aacgggtgga ccatctacac gcacgaaggc aagaaggtcg
     8461 aacccaagga cagctcgtgg agcgttcccg aggatggtgt tctgcacacc accggtgacg
     8521 agagcaagga ctattggatc tcgacggacg agaagtacgg cgactgcgtg ctgaggatgg
     8581 acttcaaggt cagccagggg gccaacagcg gcatcttcct caaggtgccc ggccacgatc
     8641 gcccggcgta cacgggcttt gaggttcaga tcattgacga cgtcggcagg ccggccgaca
     8701 agcacagcac cggctccatc tacgatctct ttgccccagt cgcgaacgtc tcgcggccgc
     8761 tgggccagtg gaacagcatc gagattacct gcaaaggctc cctcgtgacc gtggtcatga
     8821 acggcctcaa gatcattgac tgcgacttca gcctgatgac cgagccgatg ggcaagttcg
     8881 atttccccta tgccaagatg cccgcgatcg gttacatcgg catgcagacc cacggcaatg
     8941 cggtatcgtt caggaacatc aggatcaaga agctgaactg atccgagagg gcggcaagtg
     9001 tcgagccgaa agggcgggcg tcgccgattc cggccggcgg aacccgccta ttcacctgga
     9061 acacgttgct gcatcgacgt gcgcggccgc cgggtgtaga atctcactca gagtgggttt
     9121 gcccggccta gccgaaagag cagacggtcg ccgatatctc aattgttcgt ggagacgccc
     9181 catgattcgc cggattgagg ctctcaacta ccgctgcctg aaatacattt ctcaggaact
     9241 ggacgacttc catgttctcg tcggcccgaa cgcgtccggg aagaccacgt ttctggatgt
     9301 agtggctttt ttgggtgact tggtttccgg tggactggaa caggcaatgg gcggccgtac
     9361 tcagaatttc caagatctgg tctggcggcg atcaggcgac cgcatcgaat tggctgtcga
     9421 gctggagatt ccgccggaac ggcggggcga ggcagaccgg gctggccacg accgggttcg
     9481 ctacgaggtg gcgattggcc ccgaccgcga gacgggagct ctcgcgatcc tggccgagaa
     9541 ggtactcttg aagcagaccc cgcatcgcca gtccgtgcag cggttcctgt tcccggtcga
     9601 accggaaacg cccgagagcc ttattacggg aaagatcgcc cgcagcgtca agaccgtggt
     9661 gaacaaagtc ccgggaggga atgacaactt ctacgctgag acgaacaagg gctgggatca
     9721 cgctttcaag ctggggccgc gcaagtcagc cctgggcaac ctccccgacg acgagagcaa
     9781 gtttcctgtg gcaagctggc tcaagggagt cttgaaagag ggcattcaga ggcttgttct
     9841 gaatagcgcc gtcatgagaa agccgagtcc gcccagcctg cctagctctt ttctgccgga
     9901 cggggccaat ctgccgtggg ttatcgaagg gttgcgtcag cagaaagccg atcggcatca
     9961 cgaatgggtt gtccatctac agactgctct gccggatctc actgatgtca agacaatcgt
    10021 aagagaagaa gaccgacacc ggtacttggt ggcctgttat cgagatggtt tggaggtgcc
    10081 ttcctggatg gtgtctgacg gcacgttgcg tctgatggcg ctcacactgc ttgcctatct
    10141 gcccagactc cggggcgttt atttgatcga ggagcccgaa aacggcatcc acccgcgcgc
    10201 ggtcgaggct gtctttcagt ctctttccgg tgtctacgat gcacaagtgc tgctggcgac
    10261 gcactctccg gtgattctca gcattgccga ttcgtccgag gtattgtgct tctcgaaaac
    10321 ggaaagcgga gcaaccgatg tggttcgggg aacggaacat cccaaactgc gagactggcg
    10381 gggtgagacc aatctgggtg tgctcttcgc cggaggagtg cttggttgaa tacctggagc
    10441 tctgatttgg tcgttctggt ggccgatgcg agcatcgagt cagccgtgag aggactccta
    10501 gaacggaacc aggcactccc tattcgacca gtgaaggcgg acgttcacac ccacatcgag
    10561 cgagatcctg ggtgtttgct gcacagccac gacttcctcc gtccactcgc ctcgcggtat
    10621 gcccatgcgc ttgtcctctt tgaccgggag gggtgcggcc aggaaaccaa gtcacgagag
    10681 atcttggagc aggcggtgga ggcgcgtttg gctgggaccg gatggtcgaa tcgcgcgggg
    10741 gcggtggtta ttgaccctga attggaggtt tgggtctgga gcgactctcc tcatgttgaa
    10801 gatgtgctgg attggcgagg tcaggcactg ccgttgcggt catggctcgc cgaccagggc
    10861 ctgttgcagg aaggccggct caagcccgca caccccaaga gagccttgga gcgggctctg
    10921 cggctggttg gcaagccgcc ctcgtcagga atttacggag atctggcacg gcgagttggt
    10981 cttgacagat gcacagaccc ggcgtttctg aagctgcgcc gcttgctcac gagctggttt
    11041 ccgccatccg gttcggcctg agcatcagcc ttcgcgttgc atcgacttac ccccatcgcg
    11101 atgatagaat tgccgtattc ggtttgtgcc ggtcggccga tggtgagccg cggtcacaaa
    11161 gggttattgt gattgagccg ttgaggcact gctcacagag cagtgacaca cttgctcggc
    11221 ccggaatccg ctctcgctcg gcccggaatc ctttccgggc cgcggcggct ggcggaatcc
    11281 attccgcatt cggcgagaat gaggaggcga atcctccatg cggagggccc cagaaaggat
    11341 tctgggacca gcggaaatgg attctgggac cagcggaaat ggattctggg gccagcggaa
    11401 acggattctg ggaccagcga gcactgctca cagagcagtg gcacaccagt acgttcaaca
    11461 gcggcggtga atcggtgttt gtaggttggt gtttgaatgt ccacgcttga tcagttaccc
    11521 ccgacgtacg aaccacagag tgtcgagccg gagatccgcc gcatctgggc ggagtccggg
    11581 tgttttcatg ccgatccgac cgccaatgcg aacgagccct acaccatcgt catcccgccg
    11641 ccgaacgtga ccggggccct gcatggcggg cacgctctca acaacacact gcaggacatc
    11701 ctcatccgct ggcggcggat gcaggggtac aacacgctgt ggatgcccgg cacggatcat
    11761 gccggtattg ccacccaggc agtggttgag aagcgcatct tcgagactga aagaaaaacc
    11821 cggcacgacc tcggccgcga ggagatggtc cgccgcatct gggaatggaa ggagcagtac
    11881 ggcaaccgca tcatcgagca actcaagctc atgggttgct cgtgcgactg ggatcgcacg
    11941 cgttttactc tggacgaagt ctgcgctcgg gccgtccgcc ataccttctt caagctgttt
    12001 aaggacggcc tgatcgtccg cggcaagcgc ctggtcaact gggacacgca cctccagacg
    12061 gccgtggccg acgacgaggt gtaccacgag acgatccagg gccatttctg gcacttcaag
    12121 tacccggtga aggacccgcg tccgggcgaa ccgacgcacg tccacatcgc caccacccga
    12181 cccgagacca tgctgggcga cactgccgtg gccgtgcatc ccgagccgga caaggccctc
    12241 gacaagaccg aggccgagct gcgcgagaag ctggccgagg tgcccgacaa ggagaagcgg
    12301 ggcgtccagg cggcgatcga cgagattgtc gaacgccgca agacggtcct gccgttgctg
    12361 ctcaagcttc gggacatggc ccgcgacggt cgcagactca tcctgccgct ggtcaatcgc
    12421 gagatcccgc tgatctgcga cgtatgggcc aagcccgagc tgggcagcgg ctgcgtgaag
    12481 atcaccccgg cccacgatgc caacgactac gaagtcggcc ggcggcaggg cttgccgatg
    12541 atcaatgtca tgacgcccga cggcagggtc gcccggatca tcgagcccga cggggcggtc
    12601 aacccgaatt cccgcaaata cgaaggcctg cgtttcgcca ctgacgcccg caagaaggtc
    12661 gtcgccgacc tggaaggcct cggcctggtc gagaagatcg aggatcggat tgtcgagata
    12721 ggtcacagcg accgtagcaa gacgccgatt gagccattcc tctctgacca gtggttcgta
    12781 cgcatgggcg atctgcctgc tgaagaggcc gccaagatcg aaggcctccg cggcagcagc
    12841 ggcctggccc aactggcgat ggatgccgtc tgcgagaaac aacacgcgag cgctgccgag
    12901 cgcaaagcct gcggcaaggt ccacttcttt cccgagcggt acgcgaagac ctacctcgac
    12961 tggctgagcg aaaaacgcga ctggtgcatc agccggcagc tctggtgggg gcatcggata
    13021 ccggtctatc gcgggctgca gatagcagat catgaagagg ctaagcggcg tgatgccaag
    13081 gcacaggagt ggttgaaggc cggacgcatg gcgcaggcat ttggacccgt gcattcgaca
    13141 agtgcgcctc cgcctcccgg aagtccgtgg tactttgtgg gtccggccgc gctgtctcgc
    13201 gagatctacg tctgtgtgaa agacgctgac gacgatgaaa tcgtcgctga gttgcagcga
    13261 tggggcttcg tccaagatcc cgacgtcctt gacacctggt tcagcagcgc cctgtggccg
    13321 cattcgacac tcggctggcc ggcgccgaat ccgcttcttc ccgaggcgga caagctgctt
    13381 gagcggtact acccgggctc ggtgctcagc accgctcgcg agatcatcac cctatgggtc
    13441 gcgcggatgg tgctcaccgg ccggtacaac atcggcaagg tgccgttcca cgacgtttac
    13501 attcacgcgg tgatccagga cggccagggc cggccgttca agaagactct gggcaacgga
    13561 ttcgacccgg tggacatcat cgaggtctat ggagctgatg ccctgcgttt caccatggcc
    13621 aacttgagca ccgagacgca ggacatccgg atgccgatga agaaggtcaa gctcgacaac
    13681 ggccgggagg tccagtcctc cgaacgcttc gagctcggcc gcaacttctg caacaagatc
    13741 tggaacgccg cgaggttcgc gttcatgaac ctcggaaccc cccccagccc cccctcaact
    13801 gaagggggga gtgcgga
//
LOCUS       DAOTPW010000153        13771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4359_length_13771_cov_4.878390, whole genome shotgun sequence.
ACCESSION   DAOTPW010000153 DAOTPW010000000
VERSION     DAOTPW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13771
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4359_length_13771_cov_4.878390"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="P5316_14045"
     CDS             complement(<1..1683)
                     /locus_tag="P5316_14045"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR86125.1"
                     /translation="MRWAWSGFWHFVLRNRVAIFIASLFWLLYRSGTKPQRLAYPCQQ
                     VAAVNVGAFIAALIPVSLFLRKHKHGQVLPKAVIVRRQVIAAAILCVVGVVGIETYQY
                     ADQLLQPAATLPNVPPPAVEPPPAVVGIAHRPYPNWRKDGSPIPPRAPFTAAEIDGLV
                     ERAVTLAGGLDQIMVDKNSDGVLDIVLKPNLVTEIPVATYPENGIVTDPRVMASVVRL
                     VKEAAGRTGLSTLVRIAEGSACGFPWDGGNLTRTVTKTAFINCGYTTDPASGRQWFIY
                     DPTVELVDLNDSGGLDQFDPAKVTQVTIDNALCRRKYWVPNIVLHCDVLINVPTLKNH
                     SNADVTVSMKSQGIGCGPSDIYHSRPAYGVPLHLQQMKHAMHFTDVPGLNAWDPGQAS
                     GWDYPANPGSDENAIVNYTIVDMNLFRPMDFAIVDGLIGITNGPASWPATQPNPNMQL
                     IVAGKDAVAVDTTSALLMGYDPQYVRYLRWAYNRQLGTMNTSRITQMGDHVALWRYPF
                     EANWGSTHAPNGSQAETSPPMLGGISVLEGSMLVTGQTVTVNSFSDNVGVTRA"
     gene            complement(2267..3226)
                     /locus_tag="P5316_14050"
     CDS             complement(2267..3226)
                     /locus_tag="P5316_14050"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR86126.1"
                     /translation="MRLNKGLDQSVDVCRLPTSRYTGPIIDAHCHGGRSRATRRMIRA
                     GELFGIRKWVLICRVDEIAALRRRHGDRVAFNAWSEHELVGRDQPFTDANLRIVERAV
                     RAGAVSIKFWYKPEFNERSGVWFDDPRLDPVFDAIQQSGLSVLVHIADPDIWWRHRYS
                     DPLRFESKRLTYRQLTNTLERFPSLRVLLAHMGGWPENLSFLAELLDRHPNLYLDTSG
                     TKWIARELSRRPAESRDFFVRYADRLLFGSDLVAFKHATFEHHCSRYWAHRFLYERSD
                     TVRSPIEDEDAGGRVFLAGMNLPDAVLDRLYYANAASFFRLGG"
     gene            3482..4327
                     /locus_tag="P5316_14055"
     CDS             3482..4327
                     /locus_tag="P5316_14055"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRR86127.1"
                     /translation="MITVAETLSTNTLALDSIRTMGGPDGREADGLVVMADYQTAGRG
                     RFGRRWLSPRGASILCSVVIVPADQELTAANTGPSPGGHVELQALENSGRPTPTAQFS
                     GHLTLVSAIAACEAIRQSTEITPTIKWPNDLRVSGRKLGGILIESRPVAQMHRAWVIG
                     IGINCLQHAGHFPPELRTFATSLEIEASHPIERIEVAKALLQHLDHRLADVTLRASPP
                     ASFQAIRSAWEQYAEPVGQRVRLRARGREWSGTTIAVDPFGGLILQTDDGKQEWFDPL
                     QTTLL"
     gene            4749..6863
                     /locus_tag="P5316_14060"
     CDS             4749..6863
                     /locus_tag="P5316_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86128.1"
                     /translation="MILVGLAIGWQCSASQKVFAAAIGPDAETTSMVVAVALLAAAVG
                     PWLPGRAIVGIARFIQRRARRSDEANDDVIGSLLVLRATRQRDRSLVWLSISVLACLA
                     GAVSLATLLLMGPVARVYHYLLQHFFWTNLTLSALEWLGTVLLIGPPWILHGLLIATL
                     ANVAAHEHPPRQRPGLVAGILAGPGLALLLSAKWMAQTLSGAQEFMIGVLPLFVVAAL
                     AAKTSQRTDQPAGAPPATETAPELPGGTEGLIWVLLVVWGIAAVLAGTGWVTCREVGA
                     QDRPSTHTQWGWYLLTLGAGTAVAWQHARHLTRSASGCGMAAWVAGIGAGTASILAAF
                     CPGRPWSELLQLLLLGFTFGYALHYAELAWLARSASETQGFAQLAGAVLAGLAVGLIA
                     CRWWAHQALGPVGMMTAGALVMMALGGIVQIYEEDRPARIRHERLALVFASLTAAIIL
                     FPAATRRWDRWEADRAIRPLPTDLSWLATSELPSARRICLVGVDAGSSARWAGLGKAR
                     VDIIPCPGGLTQETLPRPPGRTRFVRATAFRALRLQHERYDLLYQQGQPAAPTDGYAE
                     YSIEWLHRLASLTMPDGEIVLDVPLRAMNPQALATIIATLQHAAGAPARWALVSTAGQ
                     PAIRLRTVPTTSNPTAPADDTWEAGEAITTRTRNLGIHSIQHDRLTRILQKGSLPSTD
                     DLIRWLESCRSGTSVHESSTRQ"
     gene            6988..7815
                     /locus_tag="P5316_14065"
     CDS             6988..7815
                     /locus_tag="P5316_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRR86129.1"
                     /translation="MQVNLLCIGDTVGRPGRFILSQKLPKLVEQYDLHCIICNAENAA
                     GGSGLTHQLYEKFRRYGIHLITLGDHAFRRQEIIPVLERAQDIARPANFAQAAPGKGW
                     AVYETPLGPKVAVVTVLGRLYMKPPTDCPFQAIEKTLSQIPQDVKIIAVDVHAEATSE
                     KVAIGWHFDGRVSVVFGTHTHIPTADERILPNGTAYITDVGMTGPYDSVLGRRKDRVL
                     KALLTGLHAPFDVAADDIRLCGLLVSVDSATGRATHVERIRVNSDASVSESGAAGED"
     gene            7971..9521
                     /gene="xseA"
                     /locus_tag="P5316_14070"
     CDS             7971..9521
                     /gene="xseA"
                     /locus_tag="P5316_14070"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRR86130.1"
                     /translation="MESPRPAFDPDRMAVAKARPAVPSEMTVSQLNALIKLLIADSLP
                     GTIHLVGEISNLKRADSGHFYLTLKDKNSEIRAVVWRSAASAIKFKPTDGMEVIATGH
                     VEVYEPRGQYQFIIRKLEPRGTGALDLAFRQLHARLAKEGLFDKERKKPLPRFPQRIA
                     VVTSPTGAAIRDILRTLQRRFPCVWVFVYPVRVQGEGAAEEIATAIRRLNEQSAALGN
                     IDVIIVGRGGGSLEDLWAFNEEIVARAVVAGRIPIISGVGHEVDVTICDLAADLRAPT
                     PTAAAVAAVPSAAEILNSLTDVARRLGRAMNHQVAMAQSRLAGLERLEWFRDPLAILH
                     RREQSLDEIASRLRLLLSRRTADAHRRLNRFEAILAAIQPKAFLQQEHARVVQIAYRL
                     NWALQQQVRRSERRLELAREAFLAASPAVRIPRHRQDLKALQHALEQTLRHRIENTRD
                     RLDSLEARLQATSYRGTLARGFTITRRMPDRRIITAAHQVSAGQDVATETAQGEFISR
                     VNPLPREA"
     gene            complement(9712..11955)
                     /locus_tag="P5316_14075"
     CDS             complement(9712..11955)
                     /locus_tag="P5316_14075"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HRR86131.1"
                     /translation="MSQKMNSSNSGVRVAVLALALATALGGSFAAWAKDVPTGFPPGS
                     PFGRPPGPFPPKRLSPDLPWCRYPYRTQAPSQLKTAPQKARVVTLQEIEPNDIGWQAQ
                     TVPVSSSIGDEIDATVKGSIEQGDDVDFYRIFVNKGDVVGLAVTTNRDPYDSRPDLGL
                     DPVVAVCDLTEDVLVKNDDDGGITSIYPPSSPLPRIETPYYWFYRWDSALSWIAPETG
                     DYLIRVTSFESSRRGDYELKIVLRRPAHEHQAVGATQIIFLDFDGVEDFNAVEVFGYG
                     WYLTDLSPMRDFLSRWGLTLADEAAVVDAVVAVFQERLDNIRRADLNGDRDVDSVDGH
                     MDVEIRNSRDHADPWGQPNVTRIIIGGSMYELGIPTLGIAQSIDPGNFAPEETAVVLL
                     DYLSDPDPTNLDSINAIPRAPNFSMVDAVGRVVGAIAAHEAVHTLGLWHTDNSNDVAC
                     LIDTGGNGIADEGGVGPDGIMGTGDDEMPELVPDEYDPYEGVGTDGLELTDVRVAFAL
                     STGKRLDIEPPPPVTEVPLVSISAMPKIGVAPLTVNFAGGGVDPSGGQFVVFNWDFGD
                     QTAGTGAYVSHTYNAPGTYVVRLSGVTNNAETAQTVTEVIVLSQPNQLPSARIIATPT
                     KGAAPLMVLFQAEAEDPDGEIISYGWDFGDGQGGSGQAIDHVYLAQGVYVVTLSVTDN
                     LGATQRVTKVITVTGPSPSASANEPGDTGAGLPIPLVNTCGAGAASGLVATLAGLMGM
                     ALMRRRH"
     gene            complement(12050..>13771)
                     /locus_tag="P5316_14080"
     CDS             complement(12050..>13771)
                     /locus_tag="P5316_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86132.1"
                     /translation="GETLSVSQADLPPSLMGGRDAVGVCSSTSSDAQGTPVDPFGLFP
                     PAGYMRVTGTGFYYASLSLPMLGDPTCDPGLLSPYEGGAGGYCPSGRMSPPREPYGCR
                     ILGQTTTQLGTDPPTTANRFAHSVSVCGDFLLIGAPNRTAQKADVALLRIDPSATDRP
                     ESGTVYMLQLKRPGVPAHKYMWNSQGDVVTDDQGNTSISDVSTVPTPHNFIIQDLGYV
                     RCECCPKNNFWEPGDVAFEMSRPFHIVGMPGDHIGDVTGLLDINNDGVDDFAVGGAGT
                     NGDRGAVYVIYRRQPEIESDYLLERLQLNPGNLNRLNGLFILGRPGERLGTSIAGAGP
                     RPDELKDDYNGDGYSDLLIGSPNISSGGHYAAGEVFILFGGQNLLSPQGGITIPQLRD
                     QGYGMVISGVQANGHVGSAVANAGDINKDGIADIMIAAPDASPMFDSDSDGILDSVGV
                     DLDGDRATDDLDGDGTPDDLTGAGIVYIVFGGKHLTGTISLDQIGTENLPGLVVVGKK
                     AGYHLGGGVTQGGLAVRGIGSAGDVDGDGYDDMLLSSVLADPEGKTDAGEVYLIYGFS
                     TPIKPRK"
BASE COUNT         2721 a         4634 c         3970 g         2446 t
ORIGIN      
        1 ggctcgtgtt accccaacgt tatcagaaaa gctgttgacc gtgaccgttt ggccggtcac
       61 aagcatcgag ccttcgagaa ccgaaatccc accaagcatc ggcgggctgg tctcggcctg
      121 cgagccgttc ggggcgtgag tgctgcccca gttggcctca aatggatacc gccacagggc
      181 tacgtggtca cccatctgag tgattctgga cgtattcatc gtgcccagtt gccggttata
      241 tgcccagcgg agataccgca cgtattgcgg gtcgtaaccc atcaacaagg cagacgtcgt
      301 atccaccgcg accgcgtctt ttccggcgac gatcaactgc atattcgggt tgggttgtgt
      361 ggcaggccaa gaagcgggac cgttggtgat gccgatcagc ccgtcaacga tcgcaaagtc
      421 catgggcctg aacaggttca tatccacaat cgtgtagttc acgatggcgt tctcgtctga
      481 cccgggattc gcgggatagt cccagccgga tgcctggccg ggatcccagg cgttcagccc
      541 cggaacatcc gtaaagtgca tcgcatgctt catctgttgc aggtgaagcg gtactccgta
      601 tgcgggccgc gagtggtaga tgtcgctcgg cccgcagccg attccctggc tcttcattga
      661 gacggtcacg tccgcattgg agtgattctt gagcgtcgga acgttgatca acacgtcgca
      721 atgaagcacg atgttgggta cccaatactt gcgacggcaa agcgcgttgt cgatggtcac
      781 ctgtgtgacc ttcgccggat cgaactgatc aagtcctccg gaatcgttga ggtcgaccag
      841 ttccaccgtc gggtcgtata tgaaccattg ccgcccggac gcaggatcag tcgtgtagcc
      901 gcagttgatg aaggccgtct tggtgacggt ccgcgtcaga ttcccgccgt cccacgggaa
      961 accacaggcc gagccttcgg caatccttac cagcgtactg agacctgtcc gccccgctgc
     1021 ctccttcacg agtctcacga ccgaggccat cacgcgcggg tccgtgacga taccgttctc
     1081 aggataggtc gcaacgggaa tctcggtaac cagattcggc ttcagaacga tgtcgagcac
     1141 tccatcgctg ttcttgtcca ccatgatctg gtcgagtccg cctgccaacg tgactgcccg
     1201 ctcgaccagc ccgtcgatct ctgcggcggt gaacggggcg cgcgggggta tcggactgcc
     1261 atccttgcgc cagttcggat acggcctgtg cgcgatgccc acgacggccg gaggtggctc
     1321 aaccgccggc ggaggcacgt tcggcaacgt cgctgccggt tggagcaact gatccgcgta
     1381 ctggtaggtt tcgataccga cgacaccaac aacgcacagg atcgccgcgg caatcacttg
     1441 ccgccgcacg atcacggcct tgggcaacac ctggccgtgc ttgtgtttgc gcaagaacag
     1501 actgacagga atgagggccg caataaacgc cccgacgttg acggccgcga cctgctgaca
     1561 cgggtaagcg agcctttgcg gcttcgttcc actgcgatac agaagccaga aaagagaggc
     1621 aataaagatc gccactcggt tgcgaaggac aaagtgccag aaccccgacc aggcccatct
     1681 catcttgccc accaaaccct cgttcgcgca tccagcgatg cttgaacggt atttacagca
     1741 atgatgatga taacgtgaag agttcacctg cgcgtccgtg tgccgctcct cgctcatatc
     1801 ttccctcaca cctttgtttt acaggaccgt acagatcagc tccgccaagg cgcattaaag
     1861 gccaccgaaa agtcgcccat cgagcgccga ctccacactg cattatatca ggccgaccgg
     1921 cccaatcata gttcgatatg acctgcaggc actttttttt cacgctgccc gctcttggca
     1981 cacgtggtcg caagcgaacc agaacgtccc taaaccatac aatcataagc gcttaggccg
     2041 gacagggatc cgaagacttc tggttcgcgc ccccaagcgc caagtcttcc acccgggata
     2101 gattgccgag ggcattggga caagtcaggg atacatcacg atgaagcaca aggccggacc
     2161 cgccagttct gtgatttcga cagacagggc atcacgctcg ttgcgaaccg ccgggacagt
     2221 cacccccccg caccagtgcg gccgccctgt gaccgttcac ccataactac ccgccaagcc
     2281 gaaagaaact cgctgcattc gcgtaataca gacggtccag aactgcgtcg ggcagattca
     2341 tgcccgccag aaacaccctc ccgccggcat cctcgtcctc gatcggcgaa cgaaccgtgt
     2401 ccgatcgctc gtacagaaac cgatgcgccc agtatcgcga acaatgatgc tcaaacgtgg
     2461 catgcttgaa tgcgacaaga tcgcttccga acagcagcct gtccgcatat cggacgaaga
     2521 aatcgcgaga ctccgccggc cgtcgagaca gctcgcgagc gatccatttc gtccccgacg
     2581 tgtccaggta aaggttggga tgtcggtcca acagctcagc cagaaacgac aggttttcgg
     2641 gccagccccc catgtgggcc agcaagaccc gcagcgaggg aaatcgctcc agggtgttgg
     2701 ttagctgccg ataggtcagt cgcttcgact cgaacctcaa cgggtcgctg tagcggtgcc
     2761 gccaccagat atccggatcc gcaatatgta cgagaacgga caggcccgac tgctggatgg
     2821 catcaaacac cgggtccagc cgcgggtcgt cgaaccacac gcccgaccgc tcattgaact
     2881 caggcttgta ccagaacttg atcgagaccg cccccgccct caccgcccgc tcaacaattc
     2941 gcagattcgc gtccgtgaat ggctgatccc gaccgacaag ctcgtgctca gaccatgcgt
     3001 tgaacgccac acggtcaccg tgcctccgcc gaagcgccgc aatctcatcg accctgcaga
     3061 tcaacaccca cttgcgtatt ccgaacaact caccggctcg aatcatccgc cgagtggcgc
     3121 gggaccggcc accatggcaa tgggcgtcaa tgatcggacc cgtgtaacgc gatgtcggca
     3181 ggcgacaaac gtccactgac tggtccaacc ctttgttcag tcgcacaaca acaactcctc
     3241 ggcccggcac gctcgccgcc aacgcctcat cataggacac gaccttcaaa acatacaacg
     3301 gcctcgcgac tcgtgtcatg ggcctcggaa caacgaactg ctgcaggatt tgacggcctg
     3361 cgaacgattg cagtcacggc cgcatcgggt tactgtcctg cccatgtcat tacgctcacg
     3421 cactacgctg accgccgaac aactgcggca aggttggaca ccccgacggg tcggacggca
     3481 catgatcacc gtggccgaaa cactcagcac caatactttg gcactggact ccatccgcac
     3541 aatgggcgga cctgacggcc gggaagccga cgggctggtc gtcatggcag attatcagac
     3601 tgccggacgc ggccgcttcg gcaggcgttg gctctcacct cggggggcaa gtattctctg
     3661 cagcgtggtc atcgtgccag ccgatcagga attgaccgca gcgaacaccg ggccgtcccc
     3721 gggcgggcat gttgagctgc aagccttgga gaattccggc aggccgacac cgaccgccca
     3781 gttttccggg catctgacgc tggtctcggc catcgcggcc tgtgaagcca ttcgccagtc
     3841 caccgaaatc acgccgacca tcaagtggcc caacgacctt cgggtctcag gacgaaaact
     3901 gggcggcatc ttgatcgagt cccgccctgt cgcccagatg catcgagcct gggtcattgg
     3961 gatcggcatc aactgcctgc aacacgccgg tcatttcccc cccgaattgc ggaccttcgc
     4021 cacctcgctg gagatcgagg cgtcgcatcc catcgaaagg atcgaggtgg ccaaggcact
     4081 cttgcagcac ctggaccaca ggctggccga cgtcaccttg cgggcgagcc cccccgcttc
     4141 gttccaggcc atccgctcag cctgggaaca atatgccgaa cctgtcggcc agagggtccg
     4201 gctgcgcgcc cgcggacgcg agtggagcgg cacaacgata gccgttgatc ccttcggcgg
     4261 gctcatcctg caaaccgacg acggcaaaca agaatggttt gaccccttgc agacgactct
     4321 cctctagctc taaaaaccaa aacagatatg ctgccgtaga gcacgcaccg gttatgggcc
     4381 ttcccaaccc tgaacccaca ggcggtgcgg ttctacgtcg cagagtcaaa gcggccgtca
     4441 tccaggtcgc cgaaaccaaa agaccggccg cgaggccgac cgccgtaaaa caggcgacct
     4501 tgcgatgtac ggcctggttg gatcagaaaa tggcaagacg taccggccga cgaccgatct
     4561 ccaaaacaaa ggcgtctgaa aagccgcgcc cggcaagcgc gcacgccccc gctccgcgtc
     4621 ccgccgaccc cgccgcaatc gccgaaaccc ttcttggagc acggcccgcc ggtcgcggtg
     4681 gtttcctgcc cctcatcagg gatcggagtc aatgggcgct agacggcccc ttgcccgcat
     4741 atgcatccat gatactcgta ggattggcga ttggctggca gtgctccgcg tcacagaagg
     4801 tcttcgcggc agccatcgga cccgacgccg aaacgacctc tatggtcgtg gccgtcgccc
     4861 ttttggccgc cgccgtggga ccatggctcc ccgggcgagc catcgtcggg atcgcccggt
     4921 tcattcagag gcgagcccgg cgatccgacg aagcgaacga cgatgtcatt gggtctctcc
     4981 tggtgctcag ggctactcgg cagcgagatc gctcgctggt ctggctttcc atctcagtgc
     5041 tggcatgctt ggcaggagcg gtctctctgg caacgctcct gctcatgggg cccgtcgctc
     5101 gcgtttatca ctacctcctc caacatttct tctggaccaa cttgactctc tccgccctcg
     5161 aatggctcgg caccgtcttg cttatcggtc ccccttggat cctgcacggc ctgctcatcg
     5221 ccacgctggc taatgtcgcg gctcacgagc accccccccg gcaacgtccc ggattggtcg
     5281 ccggtatcct cgcgggaccg ggcttggcgc tcctgttgag cgccaaatgg atggcgcaaa
     5341 cgctttcggg agcccaggag ttcatgatag gagtgctgcc cctcttcgtc gtggccgccc
     5401 tggccgccaa gacctcacag cgtaccgacc aaccggccgg tgcgccccct gccactgaaa
     5461 cggcgcccga gttgcccgga ggaaccgaag ggttgatctg ggtcttgctc gtcgtctggg
     5521 gaattgccgc cgtcctggcc ggtacgggtt gggtaacttg tcgggaagtc ggtgcccaag
     5581 atcggccctc aacccacacg caatggggct ggtatctcct gacgctgggc gccggaacag
     5641 ccgtcgcctg gcaacacgct cgacacctga cccgttccgc ctcaggctgt gggatggcgg
     5701 cgtgggtagc gggcatcggc gcgggaaccg cgtcaatcct ggcggccttc tgccccggca
     5761 ggccatggtc ggagttgctc cagcttctcc tcctgggatt caccttcggc tacgccctgc
     5821 actatgccga gctggcctgg ctggcacgat ccgccagcga gactcagggc ttcgcccagc
     5881 ttgccggcgc ggtattggcg ggattggccg tgggcctcat cgcctgccga tggtgggcac
     5941 atcaggccct cggccccgta ggtatgatga ccgccggcgc actcgtcatg atggccctcg
     6001 gcggcatcgt ccagatctac gaagaagacc gccccgcgcg catccggcac gagcggcttg
     6061 ctctggtatt tgcctccctg accgccgcca tcatcctctt tcccgcggcc acgcgccgat
     6121 gggaccgttg ggaagccgac cgtgcgatcc ggcccttgcc gaccgatctg agctggctcg
     6181 ccacaagcga gttaccctcc gcgcgacgca tctgccttgt cggggtcgac gccggttcat
     6241 ccgcccgatg ggcagggctc ggcaaggcaa gggtcgacat cattccctgc cccggcggcc
     6301 tcactcagga aacgctgccc cggccgcccg ggagaacgcg gttcgtccgg gcaaccgcct
     6361 tccgggcttt gcgactccaa cacgagcggt acgacctgct ctaccagcag ggtcaaccgg
     6421 ccgcaccaac cgacggatac gccgaatact cgatcgagtg gctgcaccgg cttgcctcgc
     6481 tgaccatgcc cgacggcgag atcgtgctgg acgtgcccct gcgggccatg aatcctcaag
     6541 cactcgctac cattatcgcc accctgcagc atgcggcggg cgcccctgca cgctgggccc
     6601 tggtatccac ggccgggcag cccgcaatac gccttagaac cgttcccacg acctcgaatc
     6661 ccaccgctcc tgccgacgac acctgggaag ccggtgaggc aatcacgact cgtacccgca
     6721 acctcggaat ccattcgatc cagcacgaca gactcacccg catcctccaa aaagggtctc
     6781 tgccttccac cgatgacctg atacgctggc ttgagtcctg ccgctcaggc acttcggttc
     6841 atgaatcctc gacccgacaa tagaacctca tgcaagcgcg gaaaacgacg cacggctgca
     6901 cgggccggct cttgccggac gggcaaaacc ccgctaaact gacgggtccg cggaggccaa
     6961 ggcgcacccg cggctggaaa gatccatatg caagtcaacc tgttgtgcat cggcgatacc
     7021 gtcggtcgcc ccggacgatt catcctgtcc caaaagctgc ccaagctggt cgagcagtac
     7081 gacctgcact gcatcatctg caacgcggaa aacgcggccg gcggttccgg tttgacccac
     7141 cagctttacg aaaagttccg tcgctatggg atccacctca tcacgctcgg cgatcatgcg
     7201 ttccggcggc aggagatcat ccccgttctg gaacgggctc aggacatcgc aaggccggcc
     7261 aacttcgccc aagccgcccc tggcaaggga tgggccgtct atgagacgcc gctcggaccg
     7321 aaagtcgcgg tcgtgaccgt cctcggccgg ctgtacatga agccccccac cgactgcccg
     7381 tttcaggcca tcgagaaaac cctcagccag atcccgcaag acgtgaaaat catcgccgta
     7441 gacgttcacg ccgaggcgac aagcgaaaaa gtcgctatag gctggcactt cgacggtcgg
     7501 gtcagcgtgg tcttcggcac gcatacgcac attcccacgg cggacgaacg catcctgccc
     7561 aacggcacgg cgtacatcac cgacgtcggc atgaccggcc cttacgactc cgtactcggt
     7621 cggagaaaag accgcgtgct caaggctctg ctcaccgggc tgcacgcccc cttcgatgtg
     7681 gccgccgacg acatccgact ttgtggcttg ctggtcagcg tggattcggc gaccggacgg
     7741 gccacgcacg tcgaacgcat ccgcgtgaac agcgacgcgt ctgtctccga atccggcgct
     7801 gccggcgagg actgatggca cgctccagga gcccaggcgg caccggcgtc ccacctgctc
     7861 tcctctcgcg ataccatccc agacggcgtc ttgcgtaacg aaccccttgt gcgcgatcgc
     7921 tggccgaccg ctattgcggc gcgcgatgcg aagccctacg atcccaggta atggaatcgc
     7981 cgcgtcctgc ctttgatccc gatcgaatgg ccgtcgccaa ggccaggccg gcggtgccga
     8041 gcgaaatgac cgtctcgcag ttgaatgccc tgatcaagct gctgatcgct gactccctgc
     8101 cgggaaccat ccacctggtc ggcgagatca gcaacctcaa acgggccgac agcgggcatt
     8161 tctacttgac gctaaaggac aaaaacagcg agattcgggc cgtggtgtgg cgatcggcgg
     8221 cctcggcgat caagttcaag ccgaccgacg gcatggaagt catcgccacc ggccacgtgg
     8281 aggtctacga gccacgcggg cagtaccagt tcatcatccg caagcttgag ccccgtggaa
     8341 cgggggccct cgatctggcg tttcgtcaat tgcacgcccg actcgccaag gagggactgt
     8401 tcgataagga acgtaagaaa ccgctgcccc gcttccccca gcgaatcgcc gtggtaacca
     8461 gcccgaccgg ggcggccatc cgagacatcc tcaggacgct gcaacggcgt ttcccctgcg
     8521 tctgggtctt cgtttatccg gtacgagtac agggcgaggg cgcggccgag gaaatcgcaa
     8581 cggcaatccg ccggctcaac gagcagtcgg cagccctcgg aaacattgat gtgataatcg
     8641 tcggacgcgg gggcggctca ctggaagacc tgtgggcctt caacgaggaa atcgtggccc
     8701 gagcagtggt cgccggccgg atccccatca tcagcggggt cggccatgag gtggacgtca
     8761 ccatctgcga tctggcggcc gacttgcgag ccccaacccc cactgccgcg gccgtggcgg
     8821 ccgtgccgtc cgccgccgag atcctcaaca gcctgaccga cgtggcccga cggcttggcc
     8881 gggcaatgaa tcatcaggtg gcaatggctc aatcacgcct cgccggcctt gaacggctgg
     8941 agtggttccg cgatccgctg gctatccttc atcgccgtga acaaagcctg gacgagatcg
     9001 cctcgcggct gcgactgctg ctgtcccggc gaaccgccga cgcacatcgt cgactcaacc
     9061 gtttcgaggc gatactggct gcgatccaac ccaaggcttt cctgcaacag gaacacgctc
     9121 gtgtggttca gatcgcttac aggctcaact gggccttgca gcaacaggtc cgacgatcgg
     9181 aacgacgcct cgagctcgct cgtgaggcat tcttggccgc ctcgccggcc gtccggattc
     9241 cacggcaccg gcaggacctc aaggcgctcc agcacgccct tgagcaaacc ttacgccatc
     9301 gcattgagaa tacccgcgat cggcttgatt cactcgaagc ccggctgcag gccaccagct
     9361 accgcggtac gctggcccgc ggcttcacca tcacgcggcg aatgccggat cggcggatca
     9421 tcacggccgc ccatcaagtg tccgccggcc aggacgtcgc caccgaaacc gcccagggcg
     9481 aattcatcag tcgggtcaac ccactccccc gcgaggcgtg atccgtcgcc ctggtatcag
     9541 gaagctagcg gttgcgacgc ccggatggcc ggaacaagat tatgatgtct aaagatggtt
     9601 ttaaacgcca tgcattcggc aaagcgggcg aatcgttccg actcggccgc gcagtaaccc
     9661 ccggcacatc gccgcccccc cagcgcgtcc tgccgcgagc ctcccgggcg atcagtgtcg
     9721 tcgccgcatc aaggccatac ccatcagacc ggccagtgtg gcaaccaggc ccgaagcagc
     9781 gccggcaccg caagtattga ccaacggaat cggcaggccg gcaccggtat caccgggctc
     9841 gttggcgctg gcgcttggcg acggtccggt gaccgtgatg accttggtca ctcgctgcgt
     9901 cgcgcccaga ttgtcggtca ccgacagagt caccacgtaa acaccctgtg ccaggtagac
     9961 atgatcgatc gcctgcccac tcccgccctg gccgtcgcca aagtcccagc cgtaactgat
    10021 gatctcgccg tcgggatctt ccgcctccgc ctggaacaaa accatcaatg gggcggcccc
    10081 cttggtaggt gtggcgatga tgcgagccga cggcaactga ttgggttgag acagaacgat
    10141 cacctcggtc accgtctgag cagtctcggc gttgttcgtg acacccgaca gcctcacgac
    10201 gtaggttccg ggcgcgttgt aggtgtgact cacatacgcg ccggttccgg ccgtctggtc
    10261 cccaaagtcc cagttgaaca ccacgaactg gccgcccgag ggatcaacgc cgcctccggc
    10321 gaaattcacc gtcagcggtg ccaccccgat cttcggcatc gccgagatcg acaccagggg
    10381 aacctcggtc accggcggcg gaggctcgat atccagacgc ttgccggttg acagcgcaaa
    10441 ggcgacgcga acatcagtca actcaagacc gtccgtcccg actccttcgt acgggtcata
    10501 ttcatcgggc acgagttccg gcatctcgtc gtcccccgtc cccatgattc cgtccggacc
    10561 aacgcccccc tcgtctgcga ttccgttgcc gccggtatcg atcaggcacg ccacatcatt
    10621 cgagttgtcg gtgtgccaca aaccgagcgt atgaacggct tcatgcgcgg caatggcgcc
    10681 cacaacgcga ccgacggcat cgaccatgct gaagttgggt gcccgcggaa tggcattgat
    10741 cgaatccagg tttgtcgggt ccggatcgct gaggtagtcg agcaacacca ccgccgtctc
    10801 ctcgggagcg aagttgcccg gatcgatcga ctgagcgatg ccgagcgtcg gaatacccag
    10861 ttcgtacatc gaaccgccga taatgatccg cgtcacattc ggctggcccc aggggtcagc
    10921 gtgatcgcga ctgttccgga tctcgacatc catgtgcccg tctacggagt cgacatcacg
    10981 atcgccgttg agatccgcgc ggcggatgtt gtccagacgc tcctggaaga cggccaccac
    11041 tgcatcaacc acagccgcct catcagcaag ggtcaggccc caccgagata ggaagtctcg
    11101 catcggcgag agatccgtca ggtaccagcc gtacccgaaa acctcgacgg cgttgaaatc
    11161 ctcaacaccg tcgaaatcga gaaagatgat ctgcgtggca ccgaccgcct ggtgctcgtg
    11221 ggccggccgc cgaagcacga ttttcaactc gtaatcacct ctgcggcttg attcgaacga
    11281 ggtcacgcgg atcaggtagt cgccggtctc aggcgcgatc cacgacaaag ccgaatccca
    11341 tcggtagaac caataatagg gcgtctcgat gcgcggcagc ggagaagacg gaggatagat
    11401 cgaagtgatc ccgccgtcat catcgttttt gacaagaacg tcttccgtca aatcacaaac
    11461 cgccacgacc ggatcaagac ccagatccgg ccgagaatca tacgggtcgc ggttggtcgt
    11521 caccgccagc cccacaacgt cgcccttgtt cacgaaaatg cggtagaaat cgacgtcatc
    11581 gccctgctcg atgctgccct ttaccgtcgc atcgatctcg tcgccgatgc tcgaactgac
    11641 cggaaccgtc tgagcctgcc aaccgatgtc gttgggctcg atttcctgca aggtcacgac
    11701 ccgcgccttc tggggcgctg tcttgagctg agacggtgcc tgcgtgcgat agggataccg
    11761 gcaccaaggc aaatcgggag acagcctctt cggcgggaac ggccccgggg gacgcccaaa
    11821 cggcgatccc ggcgggaagc ccgtcggcac atccttggcc catgcggcaa acgatccgcc
    11881 caatgccgtc gcaagggcta acgccaggac cgcaactcgt acccccgagt tactgctgtt
    11941 catcttctgc gacatggtct ttgtttcctg tcactcacat cggttcgtat ctttgctcac
    12001 ccggggcaac cagccccgcc gccgtcggcg cccccgtaca ctcacatgct cacttcctcg
    12061 gcttgatggg agtcgagaaa ccgtagatca ggtagacttc gccggcgtcg gtcttccctt
    12121 ccggatcggc gagaacggag gacagcagca tgtcgtcgta tccgtcgcca tccacgtcac
    12181 cggcactacc gatcccgcgt acggccaatc ccccttgcgt cacaccgcca cccagatgat
    12241 agccggcctt cttcccgacg acgaccagtc caggcaggtt ctcggtgccg atctgatcca
    12301 gactgatcgt gcccgtcaga tgcttgccgc caaaaacgat gtacacgata ccggcaccgg
    12361 tcaggtcatc cggcgttccg tcaccgtcga gatcatcagt ggcccgatct ccgtcgagat
    12421 cgacgccgac cgaatccagg attccgtcgc tatcgctgtc gaacatcggg ctggcgtccg
    12481 gagccgcgat catgatgtcg gcaatcccgt ctttgttgat gtcgcccgca ttcgccaccg
    12541 cgcttccgac gtgaccgttg gcctgaacgc cggagatgac catgccgtaa ccctggtcgc
    12601 gaagctgcgg aatcgtaatg ccgccctgcg ggctcaagag gttctggccg ccgaacagaa
    12661 tgaacacctc gccggcagca tagtggccgc ccgaagagat gttcggactg ccgatcagca
    12721 gatcggagta gccgtcgccg ttgtagtcgt ccttcaactc gtcggggcga ggaccggcgc
    12781 cggcaatcga ggtgcccagc cgctcgcccg gtcgacccag aataaacaga ccgttaagac
    12841 ggttgaggtt gccggggttc aactgcagcc gctcgagcag gtaatcggac tcgatctccg
    12901 gctggcgacg gtagatgaca tacaccgcac cgcgatcacc gttggtcccg gccccgccga
    12961 ccgcgaagtc gtccacaccg tcattgttga tgtccagcaa ccccgtgacg tcaccgatat
    13021 gatcgcccgg catgccgaca atatggaacg gacggctcat ctcaaacgcc acgtcgcccg
    13081 gctcccagaa attgttcttc gggcagcatt cgcaacggac gtagccgaga tcctggataa
    13141 tgaagttgtg aggagtcggc acggtcgaca catcgctgat gctcgtattc ccctgatcgt
    13201 ccgtcaccac gtcgccctgt gaattccaca tgtacttgtg cgcgggcaca cccggccgct
    13261 tgagctgcaa catgtacacg gtgcccgact caggccggtc ggtggccgac gggtcaatac
    13321 gcaagagagc aacgtccgcc ttctgggccg tgcggttcgg cgctccaatc aggaggaaat
    13381 cgccgcaaac cgagaccgag tgcgcaaaac gattcgccgt cgtcggtgga tccgtcccca
    13441 actgtgtggt cgtctgcccg agaatccggc agccgtatgg ctcccgcggc ggtgacattc
    13501 gccctgacgg acagtagccg ccggccccgc cttcatacgg cgacaggagg cccggatcgc
    13561 aagttgggtc gccaagcatg ggcaacgaaa gcgaagcata gtaaaagccc gtcccggtaa
    13621 ctcgcatgta gccggccggc gggaagagcc cgaacggatc gaccggagtg ccctgtgcgt
    13681 cggacgaggt cgacgagcaa acacccacgg cgtcgcgccc gcccatcagg ctgggcggga
    13741 gatccgcttg agacaccgag agcgtctctc c
//
LOCUS       DAOTPW010000154        13756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4363_length_13756_cov_5.180133, whole genome shotgun sequence.
ACCESSION   DAOTPW010000154 DAOTPW010000000
VERSION     DAOTPW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4363_length_13756_cov_5.180133"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1199
                     /gene="prfA"
                     /locus_tag="P5316_14085"
     CDS             102..1199
                     /gene="prfA"
                     /locus_tag="P5316_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRR86133.1"
                     /translation="MATASTGEPVLRKLEELRARYEAIGEEMNRPEVAGDVQRIVALN
                     KEHGKLRRMMEPYEAYRKAVALVAESQAILDDPAADPDLKALAREELGLAASQAAALM
                     EEMKAALVTGEDAAVSSVILEIRAGTGGEEAALFAGNLRDMYQRYCDRHGFSVEVMDA
                     SPSDLGGFKEIILNVKGEGVYRHLGYEGGGHRVQRVPITEAQGRIHTSAATVAVLPEP
                     EDIEIAIDWEKDVVEHVSRAGGPGGQNVNKVSSAIRLEHLPTGITVSMRDEKSQHKNR
                     AKARRVLMSRLYDHMMQSQRSQRDANRRTMIGSGDRSERIRTYNFPQNRCTDHRINLD
                     LYYLDRIMDGELEELIAALQKHDLEQRLRNL"
     gene            1276..2010
                     /gene="hisA"
                     /locus_tag="P5316_14090"
     CDS             1276..2010
                     /gene="hisA"
                     /locus_tag="P5316_14090"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRR86134.1"
                     /translation="MDILPAIDLIGGKCVRLLQGDYAQQIDYSGDPVGVAREFERAGA
                     RWLHVVDLDGARVGRPCNLPTIRRILAETSLRVEVGGGLREAETIEELIDAGVQRCIV
                     GTKALEDWEWFKQVAQRPKCANHVALGLDAKQGRLAVRGWLEQTALTAVQVAQQAAGL
                     GVSAIIYTDISRDGMLSGPNIEATLAVAKASPIPVIASGGVTTIDDVRVLCRLPLGGI
                     IIGRAIYERRLDLAEAVRIATSGEES"
     gene            2007..3563
                     /locus_tag="P5316_14095"
     CDS             2007..3563
                     /locus_tag="P5316_14095"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRR86135.1"
                     /translation="MRLTNRNVGRQVLPEVVFLLATGGVVFGGQPKVSPAQNEPDWNR
                     ILRQFGLDMDKDLRNPLLDGVTPSSLFRKADSSKPVVFTPIAAWGLETPTHAGWYVAG
                     PAVTQVPPNVERIRKEAWAYAYKQPAAEIEKSSYTPPPLKSGSVEFDPGDQPFGLWVS
                     NEGFPGEAVYSQPALVFKTNPRLRNQPYKAMIYPCCDVKEGRLIPDSYLIGWEYSTND
                     DFQDVVTRIDNVVLLPAVPLLKDVLVEDAKVRKLAGGFEFVEGPAWNLKDEALYFSDI
                     PAAHIVRYADGKVDVANTASGQSNGLMFDKGGFLIACEHAGRRVSRAVAGRAGEEIVS
                     RYDGKRLNSPNDLWIDAEGGIYFTDPRYGPRDDLEQDKEAVYYVSADKKITRIIDDLV
                     RPNGIALSPNGRFLYVLDNGADKLYRYEVRGPGRIGKGERIAYVTQPDGMTVDREGRL
                     YVAGRGGVWVLDAGGKWLGLIETPEQPANCTFGGQGWHTLFITARTSLYAIDMQTRGW
                     HVHLDGVSRE"
     gene            complement(3711..5870)
                     /locus_tag="P5316_14100"
     CDS             complement(3711..5870)
                     /locus_tag="P5316_14100"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRR86136.1"
                     /translation="MRRLRHLFQRSATVAVLAAVFLPLPDNLRADEYGVRTEDTLLRI
                     RTTDNRVFIDHLSSGQGTSWISAPSELRLIDNDIADNTPRAIAWQLDGVESQSDEQGI
                     QVRISLRGRDSTLRVRSTWKARAGPGPIEHTLAVTNNGKSPMLIPWAPSLALTLHPVR
                     LTELTHWWVEKGAGKPGPKGIHVEKILDGFAYQGVSTPYSKAETEPIPWQCIHDPSVP
                     HGLYTGIESSGCVSQMAILTAGESPSLQLSLGIDQTGDPFLAVLQPGETYVFPTAFIG
                     CYEGDVDEGCNRLHRWVERWLLPQTNEPRCPLLTCNTWGSGTGIDEQLAIKMIDECAD
                     LGIEMFHVDAGWYKQIGWWREDPAKFPNGLKPIADQAHQKGLKFGLWVAWTQGGHTAK
                     DRQALSVFEPAMREWFRFDLAEDWRSSRWRGEPVCLASREAQEWCRAELRRIIREYDL
                     DMLEHDQKMVVWDCARDAHGHTASSGDISHRATLGYYGVYDSLRDEFPNVLFENCVSG
                     GRMVDFGVLQRCHYTCATDSYDPLSLRQAFYDASYPLPPRVIEAYVADHSGPTLDTFR
                     YMLRSGMMGWCTLMLDTSKWSRQQREAGKREFELYKRHIRPQIRDGNIYHVLPRASEN
                     GWDGIQYHTPRTDSGLLFVFRAKTDQDRVVVRLKGLKADMSYRLCCADESSPPRVAHG
                     RDLMLEGLPLTLGSPQSSKIILISPAFALETGESGQD"
     gene            complement(6109..7158)
                     /locus_tag="P5316_14105"
     CDS             complement(6109..7158)
                     /locus_tag="P5316_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86137.1"
                     /translation="MSATRTWIGVVTFVVLTTALALMHAGCGNDEPKPPDRRTNTDQI
                     TTGIDDGTQAGADKDDDPKHPRALPRTGDVAGWVKTRAVSTAPAGKLADLVKDPEVLT
                     AVKAFDVRSLASCRYNQQITSAEVLFIETASPADAFGLVSVLVPEPAEWLAADRSVRA
                     VHIGSAAMRLVAQQGKVCVLVDVAGRVDDEDIRRSSRRLMDHILFSLPAADPPMLMQV
                     IPPSQQASTKLWLVRDLTILSKAAHPLIRQVATTISNQSLGLSGDATITIAAVPPIDD
                     ADPHLIWLAEYPTPDAAKAAYDRCKAASRPADSSQSLHLTVLEPKGKLLAGAWTEQAS
                     TAMPLLKNLQNALPG"
     gene            7256..8395
                     /gene="amrS"
                     /locus_tag="P5316_14110"
     CDS             7256..8395
                     /gene="amrS"
                     /locus_tag="P5316_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRR86138.1"
                     /translation="MCEAGMPREAIDVGSNLKSILEEHTMPAAPGLFRDEGDGVVTCL
                     ACGHRCRIADGKSGVCRVRFNRAGQLRVPAGYVAGLAVDPIEKKPFFHVFPGRDAVSF
                     GMLGCNFHCDFCQNWFSSQTLRDEAAGAQPSVVRAEQIVEVALERKTPVIVSTYNEPL
                     ITADWAVEVFKLARPHGIVCGFVSNGHATPEAIEFLRPYMKLFKVDLKCFKEESYRRL
                     GGQLKNVLDTITRLKQADFWVEVVTLVVPGFNDGDDELGSIARFVAGVSTDIPWHVTA
                     FHPDYRMGDPPRTPVKTLAKAFDIGKAAGLKFVYTGNLPGALGDRENTYCPDCRTLLI
                     RRQGFTIRENNMRGGRCGKCGAVIPGVWEDVAPTSSSSYPTTVSL"
     gene            8513..9214
                     /locus_tag="P5316_14115"
     CDS             8513..9214
                     /locus_tag="P5316_14115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR86139.1"
                     /translation="MSLPLAVRSEWHQHVLLLVVTDRGLELRESGLRRSRPVRVDFVG
                     GRLERRRRNVVGSERMLAKAIGFGGRRLVVVDATAGLGRDAFLMACMGCTVTMFERCP
                     VMAALLMDGVRRASGDPETSEIINNRTQVIPADARLLLPQLSESLQPDVVYLDPMFPA
                     RGKSALAKKEMRICRLVAGDDEDAGALLEVALAAARKRVVVKRPLRAPALGGSPHIVF
                     KGTTVRYDVYLIKPG"
     gene            complement(9234..9809)
                     /locus_tag="P5316_14120"
     CDS             complement(9234..9809)
                     /locus_tag="P5316_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86140.1"
                     /translation="MKQSLELLTISQTPSSEDWMNLVGRVLTEYRQYLYYALGVLIGL
                     LILGRIWRGIRRAIRRARPPQIHPSLQKYNVDYAELDRKRREAAVGVVATSTGNRLAG
                     FRIVRQVEAVFVEGFRTPDEALTALKALAVERGANALLNVRTERTAAGRCSASGDAIV
                     AAPISPGPPDPMRPLGPRPSRPPQPPPDKKP"
     gene            complement(9817..11028)
                     /locus_tag="P5316_14125"
     CDS             complement(9817..11028)
                     /locus_tag="P5316_14125"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR86141.1"
                     /translation="MGMTIWAALLIAVASIWASRHVAITRARREQLPLTPSSYDGPPS
                     KPPFVSVLIAAKDEQANIEAAVRSMLDQDYPAFEVIVINDRSSDRTGEILESIRAEYP
                     DGRLKVIHIRQLQDGWFGKNNAMREGACQAAGQWFCFGDADCRQTSRKTLSMAVRHAI
                     ENKIDFLSVLPRLETHSLWERIIQPVCGAIMVFWFHPKKVNSPHLPNAYANGAFMLMS
                     RKCYETIGGHEAVRTHINEDMQLAKLAKARGQRLFVMQNDGLYTVRMYSRFGEIWRGW
                     SRIFYGCFETFRRLRITLAMLLTTNIFPYLSLLIAACVLAVRGWAEAGTGWQMVAVAG
                     ALVVVVQQSVIFRYYRLSQTNPWLAPTFIIGAVICAGMLINAMLKLNGRTTTTWRGTT
                     YRGSQLARPSG"
     gene            12325..>13756
                     /locus_tag="P5316_14130"
     CDS             12325..>13756
                     /locus_tag="P5316_14130"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRR86142.1"
                     /translation="MRSLGWFGVFVLAGCVVRLCAAASLPQNPDIRVHISGKTGRISF
                     LRLTGDAQVLAAPPGRAAQDPAMAFLDAHGKAFGISDPAVQLAGYRVDADSLGFTHST
                     YRQYHKGVRVFGGVVKVHEDAAGRVYAANGDFLPVPDDLAVTPVVKPADAEAAAIRLV
                     NRGTPSIEHAELVVVDPAWYGGRATGPRLAYYIILRDISVVLREGVFVDARTGEILDR
                     WNMIQTSRIRKIYDAHGSSDLAGATLARSEGDPPYSLVDVNRGYDYAGDVYDYYYRAF
                     GRDSINGTGMILKVVANFYYAGICPNAFWDGQEMVFCPGTITDDIMAHEMTHGITEYT
                     ANLIYQNQPGQLNESFSDVFGELVDLFNGDAAFPGAPGGPPNWPPHPTGPGKDTPNNL
                     RTRCSYYPSYVDGVRWSMGEDADAFGGAIRDMFNPTCFGDPDMGQSPYQVCMAEDNGG
                     VHSGSGIPNHAFQLATDGGSFNGITVT"
BASE COUNT         2553 a         3848 c         4547 g         2808 t
ORIGIN      
        1 cgcaaagccg tgggcatggc acccggcgtt ctgcgatcca gaaacatgcg cgcaagccgc
       61 acagatatct ggccataggg ttctgaaggg caggtccggc gatggcaaca gcatccacgg
      121 gcgagccggt gttacgcaag cttgaagagc tgcgggctcg ttatgaggcg atcggggagg
      181 agatgaatcg gccggaggtc gccggcgacg tgcagcggat cgtcgcgctc aacaaggagc
      241 acggcaagct gcggaggatg atggagccgt atgaggcgta ccgcaaggct gtggccctcg
      301 ttgccgaaag ccaggcgatt ctggatgacc cggctgccga tcccgatctg aaagcgctgg
      361 cccgggagga attggggctc gccgcgtcgc aggccgccgc gctgatggag gagatgaagg
      421 cggcattggt gaccggcgag gatgcggccg tcagttcggt gattctggag atccgggcgg
      481 gcaccggtgg cgaggaggcg gccttgtttg ccgggaactt gcgggacatg tatcagcgtt
      541 attgcgatcg gcatggtttc agcgtggagg tcatggatgc gtcgccctcg gatctgggcg
      601 gctttaagga aatcatcctc aacgtcaagg gtgaaggcgt ctaccggcat ctgggttatg
      661 agggcggggg gcaccgggtc cagcgggtac cgataacgga ggcccaaggg cgaatccaca
      721 cctctgccgc aaccgtcgcc gtcttgccag aaccggagga tatcgagatc gcgatcgatt
      781 gggagaagga tgtggtcgag catgtcagcc gagcaggtgg acccggtggc cagaacgtca
      841 acaaggtctc gtcggccatc cgtctcgagc acctgccgac cggcatcacg gtttcgatgc
      901 gcgatgaaaa aagccagcat aagaaccgag ccaaggcccg gcgggtgctc atgtcgcgtc
      961 tgtacgacca catgatgcag agccaacgct cacagcgtga cgccaatcgg cggaccatga
     1021 tcggctcggg cgatcgcagt gaacgcatcc gcacctacaa cttcccgcag aaccgttgca
     1081 ccgaccatcg gatcaacctg gacttgtact acctggaccg gatcatggat ggcgagctgg
     1141 aagagctgat tgcagccctc cagaaacacg acctggagca gcgactcagg aacctgtgat
     1201 tgctctggcg gcgcgcattt catgccggca gcatggcatc ggaaccgttg accgtcttgg
     1261 gatgagaggt gcttgatgga cattcttccg gccattgacc ttatcggcgg caaatgtgtt
     1321 cgcctgctgc aaggcgacta cgctcaacag atcgactact ccggcgaccc ggtgggggtg
     1381 gccagagagt tcgaacgggc gggagccaga tggcttcatg ttgtcgatct ggacggagct
     1441 agggtgggtc ggccgtgcaa cctgccgacg atcaggcgga ttcttgcgga aacctcgttg
     1501 cgggtggagg tcggcggcgg cctgcgcgag gccgagacga tcgaggaact gatcgacgcc
     1561 ggcgtccaga ggtgcatcgt cgggaccaag gcgcttgagg actgggagtg gttcaagcaa
     1621 gtggctcagc ggcccaagtg tgccaaccat gtagctttgg gactggacgc caaacagggc
     1681 cggttggctg ttcgaggctg gcttgaacaa acagcgttga cggcggtcca ggtggcccag
     1741 caggcggcgg ggcttggcgt ttcggccatc atctacacgg acatcagccg ggatggcatg
     1801 ctcagcggtc ctaacatcga ggcgacgctc gcggttgcga aggcgtcgcc gattccggtc
     1861 atagcctccg gtggggtgac gaccattgat gacgtcaggg ttctctgccg gttgccgctg
     1921 ggcgggatta tcattgggcg ggcgatttac gagagacggc ttgatctggc ggaagcggtc
     1981 cggatcgcga caagcggaga agaatcatga gactcacaaa ccgaaatgtc gggcgtcagg
     2041 tactgccgga ggtggttttc ctgctcgcca cgggcggtgt cgtgttcggc ggacagccga
     2101 aggtgagtcc cgcgcagaat gaacccgatt ggaaccggat tctcagacag ttcggcctgg
     2161 acatggacaa ggacttgcgt aatccgttgc tggacggcgt gacgccgagc agtttgttcc
     2221 gcaaggccga ctccagcaaa cctgtggttt tcacgccaat tgccgcgtgg ggactcgaaa
     2281 cgcccaccca tgccgggtgg tacgtggccg ggcctgcggt aacgcaggtg ccgccgaatg
     2341 tggagaggat ccgcaaggaa gcctgggcct acgcgtacaa gcagcccgcc gcggagatcg
     2401 agaagagcag ctacacgccg cctcctttga agagcggctc ggtcgagttt gaccctggcg
     2461 accagccgtt tggcctgtgg gtcagcaatg aaggtttccc tggtgaggcg gtgtacagcc
     2521 agccggccct ggtcttcaag acaaaccccc gcctgaggaa tcagccctac aaggcgatga
     2581 tctatccttg ctgtgacgtg aaagagggca ggttgattcc tgacagctac ctgatcggct
     2641 gggaatactc cacgaacgat gattttcagg atgttgtgac gcggatcgac aacgttgtgc
     2701 tgctgccggc ggtgccgttg ctcaaagacg tgctggtgga agacgccaag gttcgcaagc
     2761 tggcaggagg cttcgagttc gttgaagggc ccgcgtggaa tctcaaggac gaagccctgt
     2821 acttcagtga cattccggcc gcacatatcg ttcgctatgc agacggcaag gtcgacgtgg
     2881 caaacaccgc cagcggccag tccaacggac tcatgttcga caaggggggc ttcttgatcg
     2941 cctgtgagca tgccggaagg cgcgtctcaa gagctgtggc cggccgggcg ggcgaggaga
     3001 tcgtgagccg ctatgacggc aagcgtctga acagcccgaa cgacttgtgg atcgatgccg
     3061 aaggcggcat ctacttcacc gacccacggt acggcccgcg cgacgatctg gagcaggaca
     3121 aagaggctgt ctactacgtg tcggctgaca agaagatcac gcgcatcatc gatgatctcg
     3181 ttcgtcccaa cggcattgca ctctcgccca acggccggtt cctgtatgtc ctcgacaatg
     3241 gggccgacaa gctctaccgg tatgaagtca ggggtccggg caggatcggg aaaggcgagc
     3301 gcatagccta cgtgacccaa ccggacggca tgaccgtgga cagggagggg cggctctacg
     3361 tggccggtcg tggcggtgtc tgggtgctgg atgccggcgg caaatggctg gggctgatcg
     3421 agactccgga gcaaccggcg aactgcacgt tcggcggtca gggctggcac acacttttta
     3481 tcacggctcg aacatcgctt tacgccatcg acatgcagac gcgaggatgg catgtgcatc
     3541 tggacggcgt gtcacgcgag taggggtgcc cgaacagagc tgccgtggtc ttttgtcgca
     3601 tcattcattt tgcgaggcgg tcatcctgtc ctgatcagta ttgtctgtgg ccgtcgtgga
     3661 tcgcttgccg ggtttcattc cggccaaagc cactcgatgg gtcggaacta tcagtcctgc
     3721 cctgactctc cggtttccag ggcaaaggca ggtgagatga gaatgatttt ggacgattgc
     3781 ggcgatccga gcgtcagggg cagaccttca agcatcaagt ccctgccgtg agccacgcgg
     3841 ggaggacttg attcgtccgc gcaacagaga cgatatgaca tgtcggcttt cagacccttc
     3901 aatcgaacga ccacacgatc ctggtccgtt tttgcccgga agacaaacag gagcccgctg
     3961 tcggtccggg gcgtgtgata ctggatgccg tcccagccat tctcgcttgc ccgtggcagg
     4021 acatggtata tgttgccgtc cctgatctgt gggcgaatgt gtcgcttgta cagctcgaat
     4081 tcccgttttc cggcttcgcg ctgctggcga gaccacttgg atgtgtccag catcagcgtg
     4141 caccaaccca tcatcccgct tcgcagcatg taccggaacg tgtccagcgt cggtcctgag
     4201 tgatcggcca cgtaagcctc aatcaccctt ggcggcagag ggtaactggc gtcgtagaaa
     4261 gcctgtcgca gcgacagggg atcataggaa tccgtggcgc aggtatagtg gcatcgttgc
     4321 agcacgccga agtccaccat tcggccgccg ctgacgcaat tctcgaagag cacattcggg
     4381 aactcgtccc gaagtgagtc atagactccg taatagccaa gtgtggcccg atggctgata
     4441 tcgcccgaag atgcggtgtg gccgtgcgcg tcgcgggcgc agtcccatac gaccatcttc
     4501 tgatcgtgct cgagcatgtc gagatcgtat tcccggatga tcctgcgcag ttcggcacgg
     4561 caccattcct gggcctcacg ggaagccagg cagaccggct cgcctcgcca tctgctgctt
     4621 cgccagtctt cggccaagtc gaaacggaac cactctcgca tggccggttc gaataccgaa
     4681 agggcctgcc gatccttcgc agtgtgtcct ccctgggtcc acgccaccca caaaccgaac
     4741 ttgagtccct tctggtgggc ttgatcggcg atcggcttaa ggccgttggg aaactttgcc
     4801 ggatcttcgc gccaccatcc gatctgcttg taccatcccg cgtcgacatg aaacatctcg
     4861 attccgaggt ccgcgcattc atcgatcatc ttgatggcga gttgttcgtc gattcctgtt
     4921 ccgctacccc acgtattgca cgtcagcaag gggcagcgag gctcattggt ctgtggaaga
     4981 agccaccgtt cgacccatcg gtgaagacgg ttgcaaccct cgtccacgtc gccctcatag
     5041 cagccgatga atgccgtggg gaagacgtag gtctcaccgg gctgcaacac ggcgaggaac
     5101 ggatcgcctg tctggtcgat tcctagactc agttgcagcg atggtgactc gccggccgtg
     5161 aggatcgcca tttgagacac gcagccgctc gattcgatac ccgtgtatag accatgcgga
     5221 acagaggggt catgtatgca ttgccatggg atcggttcgg tttccgcctt cgagtagggg
     5281 gtcgatacgc cctggtaggc aaaaccgtcg agtatcttct caacgtggat tccctttggg
     5341 cctggtttac ccgccccttt ctcgacccac caatgggtga gctcagtcaa gcgcacaggg
     5401 tgcagcgtca atgcgaggct gggggcccac ggaataagca tgggagattt cccgttgttg
     5461 gtgactgcca aagtatgctc gattggtccc ggaccggcgc gggctttcca cgtggagcgc
     5521 actctcagag ttgagtcacg acccctcaga gatatacgaa cctggatgcc ttgttcatcg
     5581 gactgcgatt cgacaccgtc gagctgccat gcgattgcgc gaggggtatt gtcagcgata
     5641 tcattgtcga tcagtcggag ttccgaaggc gcgctgatcc agctcgttcc ctgcccgctc
     5701 gacagatgat cgataaagac gcggttatct gtcgtgcgta ttcgaagaag cgtatcctcg
     5761 gtgcgaacgc cgtactcgtc ggctcggaga ttgtctggaa ggggcagaaa caccgctgca
     5821 agcaccgcta cggtggcaga acgttgaaaa agatgtcgaa ggcgcctcat cttatccttt
     5881 ccttgcgcaa acaggcacgg atttccggtt tcggctgagg cgctatctcc ccacggcaac
     5941 tcatgcagcc gtaagaacac cgcccacatg gttcttctag cagaggcgtc cgacccaagc
     6001 aagctactta ttgtccggtt cgtgttccgt tgggttggtc aggctttgtt tgttatgtct
     6061 ggttccgata cgtctcttgc gccgcgttgg gctgcgcggt gacggggtct atccgggcag
     6121 ggcattctgg aggtttttca ggaggggcat ggcggtgctt gcctgctcgg tccaggcgcc
     6181 ggccagcagt ttacccttcg gttccagaac ggtcagatgc aggctctgag atgagtctgc
     6241 cggccggctt gcggccttgc atcggtcata agctgccttg gcggcgtcag gcgtcggata
     6301 ctcggccagc cagatcaagt ggggatcggc gtcgtcgatc gggggaacgg cggcgatcgt
     6361 gatcgtagca tcgcctgaca gacccagcga ttgattgctg attgtcgtag ccacttgccg
     6421 gatgagcggg tgggcggcct tggacaggat cgtcaagtcg cggaccagcc acagcttggt
     6481 tgaagcttgt tgcgacggag ggatgacctg catgagcatc ggcgggtccg ccgcgggcag
     6541 ggaaaagaga atgtggtcca tcaggcggcg ggagctgcgg cgtatgtcct cgtcatccac
     6601 tcggccggcg acatccacga gaacacaaac ctttccttgt tgggcaacca gccgcattgc
     6661 ggcgcttcca atgtggacgg cccggacgct gcgatccgca gcgagccatt cggccggttc
     6721 aggaaccagg acgcttacca ggccgaaggc gtcggcgggc gaggccgtct cgatgaacag
     6781 gacctcggcc gatgtgatct gctggttgta gcggcatgag gcgagcgatc tgacgtcgaa
     6841 agccttcacc gccgtgagaa cctccgggtc cttgaccagg tcggcgagtt tgccggcggg
     6901 ggcggtggac accgcacgtg ttttgaccca tccggccaca tcgccggtgc ggggcagggc
     6961 ccgggggtgt ttcgggtcgt cgtccttgtc ggcaccggct tgggtgccgt catcgatgcc
     7021 ggtggtgatc tgatccgtat tcgtgcgccg gtcggggggc ttgggctcgt cattgccgca
     7081 gccggcatgc atcagagcca aggccgtcgt cagaacgacg aatgtgacaa ccccaatcca
     7141 tgttcgtgtg gccgacatgt cgtgcctcca tcaatcggaa aaggattgta tcgccctggt
     7201 gcgcatgcgt cgagatcgcc ggctgggcgg cgggctttgg cgggaatggt ataatatgtg
     7261 tgaggcaggg atgccgcgtg aggcgataga cgtgggcagc aacctcaaga gcattcttga
     7321 agagcacacc atgccggcgg cgccggggtt gtttcgcgac gagggtgacg gcgttgtgac
     7381 gtgccttgcc tgcggtcacc gctgccggat tgcggacggc aagagcggag tctgtcgggt
     7441 ccgattcaat cgggccggcc agctccgggt accggccggc tacgtggcgg gtctggcggt
     7501 cgatccgatt gagaagaagc ctttctttca cgtctttccg ggccgcgatg ccgtttcgtt
     7561 cgggatgctc ggctgcaact tccactgtga cttctgccag aactggtttt ccagtcaaac
     7621 cttgcgggac gaggcggccg gcgcccagcc gtcggtcgtc cgcgcggagc agattgtcga
     7681 ggttgcactc gaacgcaaga cccccgtcat tgtgagcacc tacaatgagc cgttgatcac
     7741 cgccgactgg gcggtcgagg tttttaagct tgcccgaccg cacggcatcg tctgcgggtt
     7801 cgtctccaac ggacacgcca cgccggaggc gatcgagttc ctgcggccgt acatgaagct
     7861 cttcaaggtt gacctcaagt gcttcaaaga agagagctac cgtcggctcg gcggtcaatt
     7921 gaagaacgtc ctggacacga tcacccgtct gaagcaggcc gacttctggg ttgaggtggt
     7981 cacgcttgtt gtgccgggct tcaacgatgg tgatgacgag ctgggaagca tcgcccgatt
     8041 tgtggccggc gtttccacgg acatcccctg gcacgtcacg gcgtttcacc cggattacag
     8101 gatgggtgat ccgccgagga cgccggtcaa aacattggcc aaggcttttg acattggcaa
     8161 ggccgcaggc ctgaagttcg tatacacagg gaatctgccc ggggcgctgg gtgaccggga
     8221 gaacacctat tgcccggact gcaggacgct gctgatccgt cgccagggct tcacgattcg
     8281 ggagaacaac atgcgtggag gacgatgcgg caagtgcggc gcggtcattc cgggtgtatg
     8341 ggaggacgtc gcgccgacgt cgagttcctc gtatccgacg accgtgtcac tgtagaatac
     8401 ctgcatgagg ccaatctccg cgggagttcc agagaggtca gtttcgatcg cggtggccat
     8461 tgacggcgac gctccatctc ttcggggtca tgcggaggcg ttgtcggcac aaatgtcgtt
     8521 gccgcttgcc gtccgttccg aatggcacca acacgtcctg cttcttgtcg tcaccgatcg
     8581 tggtcttgaa ttgcgggagt ccggcctgcg ccgctcgcgg ccggttcgcg tcgattttgt
     8641 gggtggcagg ttggagcggc gccgaaggaa cgtggtcggg tcggaacgga tgcttgccaa
     8701 ggccattggc tttgggggca gacgactcgt cgtggtggac gcaacggcag gcctcggacg
     8761 ggatgcattt ctcatggctt gcatggggtg cacggtgacg atgttcgagc gctgtcctgt
     8821 gatggccgcc ctgctcatgg acggcgtgag gcgggcgagc ggcgatcccg aaacgagcga
     8881 gatcattaac aatcggacgc aggtgatccc ggcggacgcg aggctcctgc tcccgcagct
     8941 cagcgagtcc ttgcagcctg acgtcgtcta tctcgatccg atgttcccgg caaggggcaa
     9001 gtcggctctg gccaagaagg agatgcgcat ctgccggctg gtcgcgggtg atgacgagga
     9061 tgcgggtgcg ttgttggagg tggcattggc cgccgcccga aaacgcgtgg tggtcaaacg
     9121 gccgctgcga gctcccgcgt tgggtggatc acctcacatt gtcttcaagg gcacgaccgt
     9181 tcggtacgat gtctacctga taaagccggg ctgagccctc agaaacgacc tgttcagggc
     9241 ttcttgtccg gtggcggctg cggcggccga ctcgggcggg gacccagcgg tcgcatcggg
     9301 tcgggtggtc cgggcgaaat gggcgcggcg acaatggcgt ctcctgaagc agagcaacgt
     9361 ccggcagcgg tgcgttcggt tcggacgttg agcaaggcgt tggcaccccg ctcaacagcc
     9421 agggctttca atgccgtcag tgcttcatcg ggtgtgcgga aaccttccac gaagaccgcc
     9481 tcgacctgcc ggacgatccg gaaaccggcc agacgattgc cggtagacgt ggcgacgact
     9541 ccgactgctg cttctcgccg tttgcggtcc agctcagcgt agtcgacgtt gtacttctgg
     9601 aggctggggt gaatctgtgg cgggcgggca cgccggatgg cgcggcggat ccctcgccag
     9661 atgcgaccaa ggatcagaag accgatcaaa acacccaggg cgtagtatag atactgtcgg
     9721 tattcggtca gaacacggcc gaccagattc atccaatctt cgctgctggg tgtttggctg
     9781 atggtcagca gttccagtga ttgtttcata cgcggctcaa cctgaagggc gtgccagttg
     9841 gctgcctcga taagtggttc ctcgccaggt ggtcgttgtc cggccgttca gcttgagcat
     9901 tgcgttgatc agcattccgg cgcagatgac cgctccgata atgaaggtcg gtgccaacca
     9961 ggggttggtt tggctgagtc tgtagtatcg gaagatgacc gattgttgca cgacgacgac
    10021 cagggcgccg gcgaccgcga ccatttgcca gcctgtcccg gcctcggccc accctcggac
    10081 cgccaggacg cacgcggcga tcagcaggct gaggtagggg aagatgttcg tggtcaggag
    10141 catggccagc gtgatccgca gacggcggaa ggtctcgaag cagccgtaga agatgcggct
    10201 ccatcctcgc cagatctcac cgaatcggga atacatccga acggtgtaca ggccgtcgtt
    10261 ctgcataaca aacaatcgct ggccgcgagc cttggcaagt ttggccaact gcatgtcttc
    10321 gttgatgtgg gtgcgtacgg cctcgtgtcc gccgatggtc tcgtagcact tgcggctcat
    10381 gagcatgaat gccccgttgg cgtaggcgtt cgggagatgg gggctgttaa ccttcttggg
    10441 atgaaaccag aagaccatga tggcgccgca gaccggctgg atgattcttt cccagaggct
    10501 gtgggtctcc aatctgggga gcactgagag aaagtcgatc ttgttctcaa tggcgtgccg
    10561 gacagccatg gacagcgttt ttcgggacgt ctgccggcag tcggcgtcgc cgaagcagaa
    10621 ccactgccct gcggcctggc aggctccttc gcgcatggca ttgtttttgc cgaaccagcc
    10681 atcctgaagc tgccggatat ggatgacctt aagccggccg tcgggatatt ccgccctgat
    10741 cgactccaga atctcgcccg tacgatctga gctacgatca ttgatgacaa tgacctcaaa
    10801 agcggggtag tcctgatcga gcatggagcg gaccgccgcc tcaatattgg cctgttcgtc
    10861 cttggcggcg atgagcacgc tgacgaaggg tggcttggac ggtggcccgt cgtaggacga
    10921 aggagtaaga gggagttgtt ctcgtcgggc tctggtgatg gcgacatgcc gacttgccca
    10981 aatggacgcc acggcgatga gaagtgcggc ccagatagtc atgcccattg agtgatgccc
    11041 tcacggttcg ccgtcgtggc gtcaagcttt ggatgtccag tcggcgatta tccgcgccgg
    11101 ctgtccgatc agcaaggccg ccaagactgc ctgtcttcgc gtccgccggg tcgtttcctg
    11161 ttcaaggttt cgggggagcg gcatgaggtt tcgtctgcgt gtccctttgg ctgctcgtcg
    11221 gcgggtttgc ctgccatcca cagcagggcg acggcgggcg ttcgttgctg caccgcaggt
    11281 gcggcggccg ctggcggcac gaagcggccg ttgcgatcgg cgttgtgtgc tggatcgcgc
    11341 cgatctccaa tcgcggctga atgaggatca gcgacggaag tcgtcggtcg ggtcggcagt
    11401 atctgcccaa ggtctggttt ggcaggccgt attgagcagg tttccggccg gtggttggac
    11461 tggcatgacg gctgtcccat gcgtggcagt gtgggctctg acggacgggc atagagcctt
    11521 ccggtcggac agccgtgggg cctgcgccca tgtttccaga tcgcagcgcg cttggcttgt
    11581 gccccgcaaa aaggcggccc gccttagacc ctcaggttcc caccggtacc ggcagatcgg
    11641 gtccctgttg atcgttgcca tgcttgattt ggccccgcgg tggcgttgtt ggcggggcgg
    11701 tcgcctcctt ggctactggc agacgccctc gtggccataa ttcaagcaca gatggacggg
    11761 gggttctggc ctggtcctgg ggcggatcgc ccgatacggt ctgtaatctg tttttaatca
    11821 agtacttgaa tccgattgtg ggttcgtgca attgtcctca ggtcttcagt cgctcgttat
    11881 ttatgagatt cgagttggac cgggcggcgg aggtctgtta tactggcttt ccagacggag
    11941 gaaggatcaa ggggtccctg gggcagggtc atggcgacat gggcttggac tgccaaaaag
    12001 gggtgggtgg ccgattgggg cttgtcggaa gatcaaaatc gagcattcgc gctgaaactc
    12061 aaacggatta tcgaacagcc aattgccgcg acaagttcca gaacaacaca agcggacctc
    12121 tgccgaggta tgacgttgcg tcgttactgg ttgcggaggc gacctgcgtg acggtttccc
    12181 caacggtgcc ggagctggtc gttttgcaga cgatctgtgc agatagcggt tgtgcaagcg
    12241 gattcgtggg cgaagcgtgt ggtcccccgc gtcaagagag gcgcgcacgg gtggccgcgc
    12301 atggcatgcg gaaggagtga gcgtatgaga agccttgggt ggttcggcgt ttttgttctg
    12361 gccggatgcg ttgtccggtt gtgcgcggcc gcgagcctgc cgcagaaccc ggacatccgc
    12421 gtgcacattt cgggcaagac aggcaggatc tcgtttctga gattgaccgg cgacgcacag
    12481 gttctggccg cgccgccggg ccgggctgcc caggatccgg ctatggcttt cctcgatgca
    12541 cacggcaaag cttttggcat ctcggacccg gccgtccaat tggcgggcta cagggtggat
    12601 gccgattcct tgggattcac gcactcaacc tatcggcagt accacaaggg tgttcgtgtt
    12661 ttcggcggcg tggtcaaggt tcacgaggat gctgccggcc gagtgtatgc ggccaatggc
    12721 gatttccttc cagtgcccga cgacctggct gtaacgccgg tcgttaagcc tgcggatgcc
    12781 gaggccgccg ccattcgcct cgtcaacaga gggacgccga gcatcgaaca tgcggaactg
    12841 gtggtggtgg atccggcctg gtacgggggc cgagcaacgg gaccgaggct ggcgtactac
    12901 atcattctca gggacatttc cgttgtgctg cgcgaaggcg tgtttgttga tgctcggacc
    12961 ggcgagattc ttgatcgctg gaacatgatc cagaccagca ggatccgaaa gatttacgat
    13021 gcacacgggt cctcagattt ggccggcgca actctggccc ggtccgaggg tgatccgcct
    13081 tactctctgg tggacgtgaa tcgaggctat gattacgcgg gcgacgtgta cgattactat
    13141 taccgggcgt tcggtcggga cagcatcaac ggcaccggca tgatcctcaa ggtcgtggcg
    13201 aatttctact atgccggtat ttgtccgaat gctttctggg atgggcagga gatggttttc
    13261 tgtccgggga ccatcacgga cgacatcatg gctcacgaaa tgacgcacgg gatcacggaa
    13321 tacaccgcca acctgatcta ccagaatcaa cccgggcagt tgaacgagtc tttttccgac
    13381 gttttcggtg aattggtaga cctgtttaac ggcgatgccg cctttccggg ggccccaggc
    13441 ggcccgccga actggccgcc gcacccgaca gggcccggca aggacacgcc gaacaacctt
    13501 cgtacgcggt gcagctatta cccgagctat gttgacggtg ttcgttggtc gatgggtgag
    13561 gatgccgacg cgttcggcgg agccattcgc gacatgttca atccgacttg tttcggcgat
    13621 ccggacatgg gccagagtcc ctatcaggtt tgcatggccg aggacaacgg cggcgttcac
    13681 agcgggagcg gtattcccaa tcatgccttt caactggcga ccgatggggg cagcttcaat
    13741 ggtatcacgg tgacgg
//
LOCUS       DAOTPW010000155        13705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4383_length_13705_cov_5.992821, whole genome shotgun sequence.
ACCESSION   DAOTPW010000155 DAOTPW010000000
VERSION     DAOTPW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4383_length_13705_cov_5.992821"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="P5316_14135"
     CDS             complement(<1..1009)
                     /locus_tag="P5316_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86143.1"
                     /translation="MTRIIKECWIASMALAVLSSASAPAQDKRENPKPVALDFAATLL
                     QGHSYLLTKDEAAAVDEILRQMREAENQLRNAKDDGTREQARKRQKEADNRLAAVLEK
                     NANLKRISLQGGRVNPSIFGPHEMVGDTGALLLRVDNGGEGRSFSAYDINESLPEHGK
                     TVWIDYAPTGVTWVLLGMHTLPANRITLPVEFRRPGAEPIRTLVEVIMPPVGRLKLTV
                     LSDDTGKPTPAMVRITSKATNRDRKPSNVVDLTGQFDGNGHNSSQRPANIPGRQGGMW
                     WCVPGPLDMTLPPGEYEIAIRRGIEHTPLFESFTIKSGEVTDKYGLAILRCRAERELQ
                     RI"
     gene            complement(1006..1830)
                     /locus_tag="P5316_14140"
     CDS             complement(1006..1830)
                     /locus_tag="P5316_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86144.1"
                     /translation="MASVLRTMLSGARSGRSAIRFLHVTMIAGVILWYVPPVQAHAVL
                     MTFIRHDARVSISRRNIDVTIELTFHEYPSLSVRRRMDRNHDEAITEAELKTYIDGLK
                     GSLRDALTLDVDDRALEIIPLYDPQIDLLDARNVVPAHHVLRLSWFARTPEWLRSGSR
                     IHLRYELWPEAPRIDVLSASADEGFRLVADDISGPVSLTEATTGPREIRLLCQTAPIV
                     TSESPRPAASTGQAGTLSQDKLTAGLGAALLLMIAAGMAGGIRRYIVRRIRTGGQT"
     gene            complement(1959..2513)
                     /locus_tag="P5316_14145"
     CDS             complement(1959..2513)
                     /locus_tag="P5316_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007461155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRR86145.1"
                     /translation="MLEAIGMIELTSVGIGYKVQDAMLKAADVRLVLGRTICSGKYIN
                     IVTGQVAAVQAAVEAGLNAAPDGVIDSILIPNVHPSVFAALGQSVQIGDGHQKAMGIV
                     ETFSTASVLEAADAAAKAAAVTLFRIHIAMALGGKGFVLMTGSVADCQSGVDAAAEVV
                     REKGLLVSAVVIPGPSKELYQEYI"
     gene            complement(2519..3829)
                     /locus_tag="P5316_14150"
     CDS             complement(2519..3829)
                     /locus_tag="P5316_14150"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR86146.1"
                     /translation="MPQASVEQIQSWGVVGAGGAGFPSHVKFRSKAEIIILNAAECEP
                     LLHKDKELLRAYPDEVISGLSQARQLVGAHEAVIGIKGKYHDVIELLQPKLPEGMRIV
                     ELADSYPTGDEFILVYDVTKRIIPPGRIPLAVGAVVTNVETALNVARARPVTEKFLTV
                     AGAVVEPVTVRVPVGVTFREVIGMAGGATVDDPVALVGGVMMGRLSTDFNEVVTKTTG
                     GLIVLSRDHRLARRYVLGANWKATARIGASACDQCSFCTEFCPRYLLGHPIEPHKAMR
                     SLGYSMVGEANVIGTLFCCECNLCTMMACPEDLDPSKVCVQNKRRLMAEGRKWEVEAV
                     ATRPELHLNNRRVPIARLIRKLGLADFKNKGPLLEASLITRKVRLPLKQHVGAPAAPA
                     VKVGDVVKRGDVVAKPAENQLGAVIHASINGRVSEVSDAIVIEA"
     gene            4489..5364
                     /locus_tag="P5316_14155"
     CDS             4489..5364
                     /locus_tag="P5316_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR86147.1"
                     /translation="MCLFVAVWPCVIPAALADEPKVELLWPNGAPGAKGNEDGDKPSL
                     TIYLPLKEKATGAAVVICPGGGYGNLAMDHEGHQVARWLNSNGIAGFILKYRHRGTGY
                     GHPAPLQDAQRAIRMVRSRAKEWNIVPDRIGILGFSAGGHLASTAGTHFDKGNAEAAD
                     PIDRVSCRPDFMVLVYPVISFTEPFAHAGSRKNLLGAEPDPKLVENLSNEKQVTPDTP
                     PTFLLHTDQDSGVPAENSVAFYLALRRAKVPAEMHIYAKGPHGFGLGKKGEAVATWPD
                     RCVDWMREQGLLDRK"
     gene            5571..6134
                     /locus_tag="P5316_14160"
     CDS             5571..6134
                     /locus_tag="P5316_14160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRR86148.1"
                     /translation="MRYTMILSVLILFAGCAATRSPTAGPAREALMNGYSTARVDFGI
                     VVSDIDKAAQFYKNALGLVEVEGFDVPADMGRNSGLSDNQPFHVRVFKVADDENATQV
                     KIMQFKGAPGKRVDNTFIHSSFGIRYLTFYVQDIAAAAERVRRAGGTVIANGPVELPK
                     ELAPGVFLAVVRDPDGNMVELVGPKKQ"
     gene            complement(6300..7604)
                     /locus_tag="P5316_14165"
     CDS             complement(6300..7604)
                     /locus_tag="P5316_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86149.1"
                     /translation="MPGTRLKNAFLVTPEGVVRADMVLRNGRIVLPDDSAACDQEIDL
                     TGKYVVPGFVDIHFHGFNLFEFTLGMYDPATETFDVSESAYRHGFDMLQKRLCQYGVT
                     GFYVGTWAAPVDTLDRCFRYLSEHMARSSGTINGARLRGGFLEGSFLNPRLCGAMNPE
                     YVLEHSRESFDRIQSRNMIKLANVVPDYGRRSCDLIEYLTAKGIVVGAGHVDATCVQW
                     ADAIKAGLKYAVHFTNGPTGGSTKVFDGGGSIEAVLSFDEIYAELILDGYHVNPAYVR
                     DIMRRKGLDRIIGITDAMYVAGSGMTGEFSLGGICGEISENGEYFAVVGKKNTLFSAN
                     ITMDRVFANILNWLSRPLPGIWTRKHDALPFEEAVTAAARICSTNACGLTGLNREGYG
                     TLATGAPADLAVLSIAGSAGSYSVAVERTIVGGTTVYCVGAR"
     gene            7872..11267
                     /locus_tag="P5316_14170"
     CDS             7872..11267
                     /locus_tag="P5316_14170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRR86150.1"
                     /translation="MADPQADSRDERIGRILNEYLDRAQRGEPVDEQELLKTNPDIAD
                     ELREQFSLVRHVSPLSSHGAQGARINIAVPPDLLPGYEILGEIHRGGQGVVYRALQKA
                     TRRKVAIKVMREGPFAGWRDRTRFEREVRILGALNHPSIVTIHESGTASGYHFFVMDY
                     IEGPPPDVWLSSRPRSIKEILRLFAEICAAVNEAHIKGIIHRDLKPGNIRIDEAGRPH
                     ILDFGLAKLTAQEGGGADAADMTVVGQFVGSLPWTSPEQAQGTLDSIDMRTDVYALGV
                     ILYQMLTGTFPYEVTGPMRDVLNRIVSDEPVRPSTVRKGIDRDLEAIVLRCLRKEPHR
                     RYQTAGELERDIERYLKGEAVSARSDSGLYVLRKTLRRYRVPVAVAAAFAVLLGVSLL
                     ASISLWRQAVAERDRAIAAQREQDRLRSLADAKASEAEQARGFAERERIRAEDQAEQL
                     RRTTYVNRMVLAQTAYEQRYLTQTHQLLDACPTDLRGWEWHYLSRLSKEPTLLDVRAD
                     AECVVSLALSADGRRLATGGCDGVIRIWDSTTGAAITQLKGHADQVNALAFSPDDQWM
                     ASGGRDKTLRLWDWRRGSHRVLQEGRQYLNAVQFSPDGSGLAAAGQGRVLAFWDVQSG
                     QLIWETPAHDKEIACVAYRPDGRQVVSGELLNPSAGDCKIRVWDVATGQSIREFSGPP
                     GAVLCIAFSPDGTRMAVGGGVSPARRDAQGTLKVFDGATWEELLSLRGHEGFVDAVAF
                     SPDGTLLASTGVPRAPTFAMESDRTLRVWNATTGEELYTHSAHSRGGRGVAWSQDGSR
                     IFSVGMDGRLKAWPRTLPPEARVWQGYAGSVLRVVFSPDGKRIASCGAESENDLATEQ
                     ASDDAIRVWDRQTGTLLMTLAGHTGAVLALVWSPDGRYVASGSADRTIRVWDASDGSR
                     QFLFPSPDGAVRDVAFSPDGTLVAGVAGYATTVWSLVDGEEQLHLWHNATPLTIAFSP
                     NGRWIAAGLARGEIGIWDRESAGEVRTIGFEKPLNGAWFGPASTVIASAHTDGSINLC
                     DVKTGSLLSAMQGPQQSVEWLDFSPDGRRIVSCSADTIIRVWDVASASQVYTIRAHDA
                     PVMCARFSPDGMAIATCGQDGTIKIWEAQTDTLPRPASRTGAE"
     gene            11680..12306
                     /locus_tag="P5316_14175"
     CDS             11680..12306
                     /locus_tag="P5316_14175"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR86151.1"
                     /translation="MEEAKFRKTMAEVQAGKVAAMDSLLLASYDPLRAAIAQQMSDVL
                     RKAQLEPEDVIQETYAAAWASLPKSQFESFEAFLGWLRQIAENKLIDMHRAIVADKRD
                     VRRQGAAWGVKSGSYVDLLDQVTSPMATPSRGAARNEALAVLMVRLQQLPEDYRQVIH
                     WRLIEGLPVAEVAGRLGRSEAAVHMLFHRALKQLRELMGASSDYLSQA"
     gene            12468..13154
                     /locus_tag="P5316_14180"
     CDS             12468..13154
                     /locus_tag="P5316_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86152.1"
                     /translation="MNVVSQADTMPVQHLGREHVAINSMEAAVKCPFCAEEIQDAAIL
                     CRFCGAVKQDGQWYPPARPTVEAPRRKGSFTIKSAGVLFIISGAYTLITATSEVPLFG
                     AMRGGLVALAYNLGFAALFLGIGIGLVIGKPWGYGLILSGTALYSVDKILLMLDKPAQ
                     EAYFAAESSVQSLAAFIGLQEADLQAVWSQAIIMGCLISLGCWWGFALYIYVRRDYFR
                     QSAPRAAVGR"
     gene            complement(13254..>13705)
                     /locus_tag="P5316_14185"
     CDS             complement(13254..>13705)
                     /locus_tag="P5316_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86153.1"
                     /translation="VPVEIVAPDHPAVDLVIDEKLNPMALGQLSATARLFARSETKST
                     LELTIHGQGLPISIASVVVVKLAGQEVWRGSFCVGPTGRFDYGGLGGEIARPAPHVTT
                     LDVILTPSVELAEKATLYDRIWGQEILLENVPLQREDLEEGESALTS"
BASE COUNT         2624 a         4127 c         4302 g         2652 t
ORIGIN      
        1 gaatacgctg caattcacgc tctgcgcggc agcgtaaaat ggctaaaccg tacttgtctg
       61 tgacctcgcc ggatttgatg gtgaagctct cgaaaagcgg ggtatgctcg ataccgcggc
      121 ggatcgcgat ctcatactcg cccggcggca aggtcatatc caacggtccc gggacgcacc
      181 accacatgcc gccctgacgg ccggggatgt tcgccggccg ctggctgctg ttgtggccgt
      241 taccgtcgaa ttggccggtc agatcgacga cgttgctcgg tttgcggtcg cggttggtgg
      301 ctttcgaagt gatgcggacc atggccggcg tgggcttgcc ggtatcgtcg gacaaaaccg
      361 tcaactttaa gcgcccaacc ggtggcatga ttacctcgac cagcgtgcga atcggctcgg
      421 cgccgggacg ccggaactcg accggcaacg tgatcctgtt ggctggaaga gtgtgcatgc
      481 ccagcagcac ccacgtgacg cccgtcgggg cgtaatcgat ccaaacggtt tttccgtgtt
      541 ccggcaatga ctcgttgatg tcataagcgg aaaagcttct tccctccccg ccattgtcca
      601 cgcgcagcaa cagcgcgccg gtatcgccga ccatctcgtg gggaccaaag atcgaagggt
      661 ttaccctgcc accctgcaac gagatgcgct taaggttggc gttcttttcg agcacagccg
      721 ccagtctgtt gtctgcctct ttctgacgct ttcgggcttg ttcgcgggtc ccgtcgtcct
      781 ttgcgttgcg caattgattt tccgcctcgc gcatctggcg aaggatctcg tcaactgcgg
      841 cggcctcgtc cttcgtcagc aagtacgagt gtccttgcag cagtgtagcg gcaaagtcca
      901 atgcaacagg cttggggttt tcgcgtttgt cctgagcggg cgccgaagcc gaagagagca
      961 ccgcaagggc catcgacgcg atccagcatt ctttgatgat tcgtgtcatg tctgacctcc
     1021 cgtccgtata cgtcggacaa tgtatcgtcg aatgccgccg gccatgccgg ccgcaatcat
     1081 gagcaagagg gctgcgccga gtccggcggt caacttgtcc tggctcaaag tgccggcctg
     1141 accggtggaa gccgccggac gtggtgattc ggatgtcaca ataggggccg tctggcataa
     1201 aagtcgaatc tctctgggcc ctgtggtcgc ttcggtcaat gaaacgggac cgctaatgtc
     1261 atctgcgacc aggcgaaacc cctcgtctgc gcttgcgctg agcacgtcga ttcgcggggc
     1321 ctcgggccag agttcgtacc gcaaatgaat gcgactgccc gaacgcagcc actcaggtgt
     1381 tctggcgaac caggaaagcc gcaacacgtg atgagccggg acgacgtttc gtgcgtcgag
     1441 aagatcaatc tgaggatcat acagtggaat aatctccagg gcccgatcat ccacgtccag
     1501 cgtcagagcg tcgcgcagcg aacccttcag gccgtcaatg tacgttttga gttcagcctc
     1561 ggtgatcgcc tcatcgtgat tacgatccat tctgcgccgc acgctcagcg agggatactc
     1621 gtggaaggtc aattcgatgg taacgtcgat attgcgccgg ctgatgctga ctctcgcgtc
     1681 gtggcgaata aaggtcatca aaacggcgtg ggcctgaacg ggcggcacat accacaagat
     1741 cacgcccgcg atcatcgtga catgaaggaa tcggatcgcg gatcgtccgg accgcgcgcc
     1801 ggaaagcatc gttctcagca cgcttgccac gaagttgttc tccatacgac cggcgtgtgc
     1861 cctgttgaac accgccgaga tgagcccggc ggcttgtggt tcgatgacac tcgtctcttt
     1921 tgccgttgcc ggggcgcggt cttcggccgc cgagcggctc agatgtactc ctggtacagt
     1981 tccttactcg ggcccgggat cacgaccgcg gacaccagca ggcccttctc gcgtaccacc
     2041 tcggcggcgg catcgacgcc tgactggcaa tcagccaccg atccggtcat cagtacgaat
     2101 cccttgccgc cgagggccat ggcgatatgg atacggaaca gggtcacggc cgcggccttg
     2161 gcggcggcgt cggcggcttc caggacgctg gctgtggaga aggtctcgac gatacccatg
     2221 gccttctgat gaccatcgcc gatctgtacc gactggccaa gggccgcaaa gacgctcgga
     2281 tgcacgttgg ggatcaggat cgaatcgatg actccgtcgg gagcggcgtt gaggcctgcc
     2341 tcgacagcgg cctgaaccgc cgccacctgt ccggtgacga tgttgatgta tttgccggag
     2401 cagatggtcc gtccgagcac cagtctcacg tccgctgcct tgagcatggc atcctggacc
     2461 ttgtacccga tgcccacgct ggtcagttca atcattccga tggcttcaag catggcagtc
     2521 acgcctcaat cacaatcgca tccgacactt cgctgacccg cccgttgatg ctcgcgtgga
     2581 tcacggctcc caactggttc tcggccggct tggcaacgac atcgccgcgc ttgacgacat
     2641 cgccgacctt aacggccggc gcggcaggcg caccgacgtg ctgcttgagc ggcaatcgca
     2701 ctttcctggt gatcagcgaa gcttccagca gcggtccttt gttcttgaag tcagccaggc
     2761 cgagcttgcg gatcagccgt gcgatgggca cgcgccggtt attgaggtgc agttccggcc
     2821 gcgtcgcgac agcctcaacc tcccacttgc gcccctcggc catcagccgg cgcttgttct
     2881 ggacgcatac cttgctgggg tcgaggtcct cggggcaggc catcatagtg cacaggttgc
     2941 attcgcagca aaaaagcgta ccaatcacat tggcctcgcc gaccatgctg tacccaagag
     3001 aacgcatcgc cttgtgcggc tcgatggggt gacccaggag atatcgcggg cagaactcgg
     3061 tgcagaacga acattgatca caggcactgg ccccgatgcg ggcggtggcc ttccagttgg
     3121 ccccgagcac atagcgccgg gccagacgat ggtccctgga cagaacgatg agtccccccg
     3181 tggtcttggt gaccacctcg ttaaagtcgg tggacaacct gcccatcatg acgccgccga
     3241 ccagggcaac cggatcgtcg accgtggccc cacctgccat gccaatgact tctcggaaag
     3301 tcacaccgac gggtacccgc accgtgaccg gctcaacgac ggcgccggcg acggtaagga
     3361 atttctcggt taccggcctg gctcgtgcga cgttcagggc ggtttccacg ttagtgacca
     3421 ctgcacctac cgcgaggggg attctgcctg gtgggatgat ccgctttgtc acatcataca
     3481 cgaggatgaa ctcgtcgccg gttgggtagc tgtcggccag ttcgacgatc cgcatgccct
     3541 cgggcagctt gggctgaagc agttcgataa cgtcgtggta cttgcctttg ataccgatta
     3601 cggcctcgtg ggccccgaca agttggcgag cctgggacag gccgctgatt acctcgtccg
     3661 ggtaagcccg gagcaattcc ttgtctttgt gaagaagagg ctcgcactcg gcggcgttca
     3721 ggatgatgat ctcggccttg gaccggaact tcacatggga cggaaagccg gccccgccgg
     3781 ccccgaccac accccacgac tgaatctgtt caactgatgc ttgtggcata tctctcgtcg
     3841 ccagtgactc ttgccgctcg gttcccccgg cggcgccata aggtaacatg atcgccacac
     3901 aaccgccagt tgtcttcggc ggggctaatg cccgcgggcc cgcgacagaa ccggcggcac
     3961 ttctggcggc tgacccggtt gtcgtgaacg caagccagac ggcttcagcg gtctttcccg
     4021 aagggcaaac aggccggccg ttttacgact ggttcatggt cggccaattc ctgcttcacg
     4081 gtgttacccc atcgaggcct ttttagcggg cttgtccttg tcgagtaagc ccgttgaaac
     4141 gggctgagac cacggaaagg aagaagaacc gcaggcgacc gccaaccggc cgtacaacgg
     4201 ccggcctatc agcccgcctt tggcgggaaa acctgaaggg ccttgatcgc ccaacctgga
     4261 ccggtcggca tgcccgccca gactgtcacg cacccaacgc cctcgtcgat cctgttttgg
     4321 gaggatcagg taccctgcgg cttgagactg gggggtagct gaagtaactt gtgtgatggc
     4381 ggatcgtcgc ttcactctca ccggccctgc gcagtaggat ttcgctatga ggtcagaatc
     4441 gcgcatctgg ccggaaagga caattccatg ttgcagtcga tgaggagtat gtgtctgttc
     4501 gttgccgtct ggccatgtgt gattcccgca gcgctcgcag atgagccaaa ggtcgagctc
     4561 ttgtggccca acggggcgcc cggagccaaa gggaacgagg atggagacaa gccgtcgctg
     4621 acgatctatc tacccctgaa ggagaaagcc accggcgcgg ccgtggtgat ctgcccgggc
     4681 gggggatatg gcaacttggc gatggaccat gaggggcacc aggtggcccg gtggctcaac
     4741 tcgaacggca tcgccggctt catcctgaag taccgccacc gcggcacggg ttacgggcac
     4801 ccggcgcctt tgcaggatgc ccaacgggcc atccgcatgg ttcgcagccg ggcgaaggag
     4861 tggaacatcg ttcccgaccg gatcggcatc ctgggtttct cggccggcgg gcacttggct
     4921 tcgacggccg ggacgcattt cgacaagggc aatgccgagg ccgctgatcc gatcgaccgg
     4981 gtcagttgcc ggcccgactt catggtcctg gtctacccgg tgatctcctt caccgaaccg
     5041 ttcgcccacg cgggctcgcg caagaacctc ctgggcgccg agccggaccc caagctggtc
     5101 gagaaccttt cgaacgagaa gcaagtcacg cccgacacac cgccaacgtt ccttttgcac
     5161 acggatcagg atagcggagt gcctgccgag aacagcgtgg ccttttacct cgccttgcgg
     5221 cgcgccaagg tgccggccga gatgcacatc tatgccaagg gcccgcacgg gttcggcctg
     5281 ggaaagaagg gcgaagcggt cgccacatgg cccgatcggt gtgtcgactg gatgcgcgag
     5341 cagggcctgc ttgatcggaa atgaatcggc tggcatgctt aagactgcaa gacaagcaat
     5401 gcccctttgg caagcgggtc ttgtttgtca gtgggcgtgc aggagaagta cgaattggct
     5461 ccgggtgagg ctcaagaacc tgcgacccgc catctggccg gtccgatggc gcagtcgatc
     5521 gtcgagttcg cggacaagac tcgccttgcg agattgtcaa ggaggtttcc atgcgttaca
     5581 cgatgatatt gtcggtcctg attctgtttg ccggttgcgc ggccacccgg tctccgacgg
     5641 ccgggccggc gagggaggct ctgatgaacg gttatagcac tgcgcgcgtt gatttcggga
     5701 tcgtcgtcag cgacatcgat aaggcagcgc aattctacaa gaatgcgctc gggctcgtgg
     5761 aggtggaagg tttcgatgtt ccggccgaca tgggcaggaa ctcgggtctc agtgacaacc
     5821 aaccctttca cgtgcgggtt ttcaaggtgg ccgacgacga aaacgcaacc caggtcaaga
     5881 tcatgcaatt caagggtgct ccgggcaagc gagtggataa cacgttcatt cattccagct
     5941 tcggcatccg ctacttgact ttttacgtcc aggatatcgc cgccgccgct gagcgggtcc
     6001 gcagggccgg cggcacggtc atcgccaacg gcccggttga actgcccaag gaactggcgc
     6061 cgggcgtctt tctggctgtc gtgcgtgatc cggacggaaa catggtcgaa cttgtggggc
     6121 cgaagaaaca gtgagcctcg ggcaacgggt cgattcgggc ccgcccgccg cacgtgcgga
     6181 tgccgaggaa atgccgcggc gagcgagaag acgacgtcgc ctgcaattcc cctccgaaag
     6241 cgagtcgtga tcatgccgta tcgcgcggcg attctcgccg taatcgatag ctcccaagct
     6301 cagcgggcgc cgacgcagta cacggttgtg ccgccgacaa tcgtgcgctc gaccgcaacg
     6361 gaataggagc ccgcgctgcc ggcgatcgaa agcactgcga gatccgccgg cgctccggtg
     6421 gccagcgtcc cgtaaccttc gcggttcaag ccggtcaggc cgcacgcgtt cgtcgaacag
     6481 attctcgcgg cggccgtcac ggcctcctca aaaggcagcg cgtcgtgctt gcgggtccat
     6541 atgcccggca aggggcggct gagccagttc aggatgttgg caaaaacacg gtccatggtg
     6601 atgttcgcag agaacagcgt gttcttcttt ccgaccacgg cgaagtattc tccgttctcg
     6661 ctgatctctc cgcagatacc gccgagactg aattcgccgg tcatacccga gcccgccacg
     6721 tacatggcgt cggtgatgcc gatgatcctg tccaggccct tgcgtcgcat gatgtcgcgc
     6781 acgtaagcag ggttgacgtg atagccgtcg agtatcagtt ccgcgtagat ctcatcgaac
     6841 gagagaaccg cctcgatgga gccgccgccg tcgaacacct tggtggaacc gccggtcggg
     6901 ccgttggtga aatgaaccgc atacttgagg ccggccttga tggcatcggc ccattgcacg
     6961 caggtggcgt cgacgtggcc ggcacccacg acgatgcctt tggcggtcag atactcgatc
     7021 aaatcgcagc ttcggcggcc gtaatcggga acaacgttgg ccagcttgat catgttcctg
     7081 ctctggatgc ggtcgaacga ttcccgcgag tgctcaagta cgtattccgg attcattgca
     7141 ccgcataagc gcgggttgag gaacgaaccc tcgagaaaac cgccgcgcag cctcgcaccg
     7201 ttgatcgtgc cggatgaacg ggccatgtgc tcgctcaggt acctgaagca cctgtccagc
     7261 gtgtcgacgg gtgcggccca agttccgaca tagaaaccgg tcacaccgta ctggcaaagc
     7321 cgtttctgca gcatgtcgaa gccatgccga tacgcggact cgctgacgtc gaaagtctcc
     7381 gttgccggat cgtacatgcc cagggtgaac tcgaaaaggt tgaaaccgtg aaaatggatg
     7441 tccacgaacc cgggaacaac atacttgccg gtaaggtcaa tctcctgatc gcaggccgct
     7501 gaatcgtcgg gcaggacgat tctgccgttt ctcaaaacca tgtcggcccg cacgacgcct
     7561 tcgggtgtaa ccaggaacgc gttcttcaat cttgtgccag gcattcaatc gtatccttcc
     7621 cgtgacctgc cgccgtccat gataccacgg cccatgtccg tccgtcttgc cttggccggc
     7681 tcaggggcgg ccgtcgcgga gaaaggctgc cctgcggtgc agtgtgagca ctcaagtcca
     7741 cgacccgcaa ccctggcagg cccgaccgca ttcgttgacg ccgcagaaca gccgacatac
     7801 aagacccagg ggccgttttg tgcgataata tcaggggcct tcggcaggca gtcgaggcta
     7861 ggtgattctg catggcagac ccgcaagcgg attctcgaga cgagcggatt ggccgcatcc
     7921 tgaacgaata cctggaccgc gcccagcggg gtgagccggt tgacgagcag gaactgctga
     7981 aaaccaaccc ggacatcgcc gacgagttgc gggagcaatt cagcctcgtc cgccacgtga
     8041 gtccgttgtc gtcccacggt gcccaagggg cacggataaa cattgccgtg ccgccggatc
     8101 tgttgcccgg ctacgaaatc ctgggcgaga ttcaccgagg cggtcagggc gtcgtctacc
     8161 gggccttgca gaaagcgacc cgacgcaagg tggccatcaa ggtcatgcgc gaggggccgt
     8221 ttgccggctg gcgagaccgc acccgcttcg agcgcgaagt ccggatcctg ggagcgctga
     8281 atcatcccag tatcgtgacc atccacgaga gcggcacggc gtcgggatac cacttcttcg
     8341 tcatggacta tattgagggt ccgccgccgg acgtgtggct cagcagtcgc ccgcgatcga
     8401 tcaaggaaat cctccggctg ttcgccgaaa tctgcgccgc cgtcaatgaa gctcacatca
     8461 aaggtattat tcatcgcgac ctcaagcccg gcaacatccg cattgatgag gcaggcaggc
     8521 cgcatatcct ggatttcggc ttggcgaaat tgaccgccca ggaaggcggc ggagccgacg
     8581 cggcagacat gaccgtagtc ggccaattcg tcggcagcct gccctggacc agccccgagc
     8641 aagctcaagg aacactcgac agcatcgaca tgcgcaccga tgtgtacgcc ctcggtgtga
     8701 ttctctatca gatgctgacc ggcacgttcc cgtatgaggt caccggcccc atgcgcgacg
     8761 tgctcaatcg gatcgtcagc gacgaaccgg tgcgtccgag caccgtccgc aaaggcatcg
     8821 accgtgatct cgaggcgatc gtcctccgct gcttgcggaa ggaaccgcat cgtcgatacc
     8881 agacggcggg cgaattggag cgcgatatcg agcgctattt gaagggcgaa gcggtgtctg
     8941 ccagaagcga cagcgggctg tatgttctcc gcaagacgct gcgacggtat cgtgttccgg
     9001 tggccgtggc agcggcattt gccgtgctcc tcggcgtgtc gctgctcgca tcgatatccc
     9061 tctggcgaca ggcggtggcc gagcgggacc gcgccatcgc cgcccagcga gagcaggatc
     9121 ggttgcgctc cctcgcggat gcgaaggcat ccgaagcgga acaggcaaga ggatttgccg
     9181 aacgagagcg aatccgcgcc gaagatcagg ccgaacagct tcggcgaacg acctatgtca
     9241 atcggatggt cctcgctcag accgcgtatg agcagagata cctgactcag actcaccagt
     9301 tgctcgatgc ttgccccacg gacttgcgag gttgggagtg gcactacctg tcacgactgt
     9361 caaaggaacc gactctgctc gacgtccgcg cggatgcgga gtgtgtggtc tcgctggcct
     9421 tgagtgcaga cggtcgtcgc ctcgccaccg gcggatgcga cggcgtgatc agaatctggg
     9481 acagcaccac gggcgcggcg atcacgcaac tgaagggaca cgccgaccag gtcaacgccc
     9541 tcgcgttcag ccccgacgac cagtggatgg cttcgggtgg tcgcgacaag accctgcggt
     9601 tgtgggattg gagacgcggc tcgcacaggg ttctccagga aggccggcag tatctcaatg
     9661 ccgtgcagtt cagtcctgat ggttcaggtc ttgccgctgc cgggcaaggc agagtgttgg
     9721 ctttctggga cgtccagagc gggcagctca tctgggaaac gcccgctcac gacaaggaaa
     9781 tcgcgtgcgt tgcctatcgt cctgacggca ggcaggtggt cagcggtgaa ctgctcaacc
     9841 cctccgccgg cgactgcaag atcagagtct gggatgttgc caccggccaa agcatacggg
     9901 agttctccgg cccccccggt gcggtgctgt gcatcgcgtt ttcgcccgac gggacccgga
     9961 tggccgtggg cggcggcgtt tctcctgcca ggagggatgc tcagggcaca ctgaaggttt
    10021 tcgacggcgc cacctgggaa gaactgctgt cgctccgcgg ccatgaggga ttcgttgatg
    10081 cggtggcctt cagccccgac ggcacactgc tggcctcaac cggcgttcct cgcgcgccga
    10141 cattcgccat ggagtcggat cgcactttga gagtctggaa cgcgaccaca ggtgaggagt
    10201 tgtacactca ttccgcccac agccgcggcg gcaggggggt ggcctggagc caggacggaa
    10261 gtcggatctt ctcagtcggc atggatggca gactgaaagc ttggccgagg acgcttccgc
    10321 cggaagcccg tgtgtggcag ggatacgcag gttcggtgct ccgcgttgtg ttcagcccgg
    10381 atggcaaacg catcgcttcc tgtggagcag agagtgaaaa cgatctggcg acggagcagg
    10441 cgtcggacga tgccatccgg gtatgggaca ggcaaacggg tacgctgctc atgacgctgg
    10501 ccgggcacac cggcgccgtg ctcgctttgg tctggagccc tgacggcagg tatgtcgcat
    10561 cgggaagcgc ggatcgaacc attcgcgtgt gggatgcgag tgacggttct cgacagttct
    10621 tgttcccaag cccggacgga gccgtgcgag acgttgcctt ttcgcccgac ggtaccttgg
    10681 tggccggcgt cgccggttat gccaccactg tctggagcct ggtcgatgga gaggaacagc
    10741 tacacctgtg gcacaatgct acgcctctga cgattgcctt cagccccaac ggccggtgga
    10801 ttgcagccgg tctggcacgc ggtgagatcg ggatctggga tcgcgaatcc gcgggcgagg
    10861 tgcggacgat tgggtttgag aaacctctga acggcgcgtg gttcggtccc gcttccacag
    10921 tcattgcgag tgcccatacc gacggcagca tcaatttgtg cgatgtgaag acgggcagcc
    10981 tgctatccgc gatgcaaggt ccccaacaat cggttgaatg gcttgacttc agcccggacg
    11041 ggcggcggat cgtctcatgc tccgcagaca cgatcatcag ggtctgggac gtcgccagtg
    11101 ccagccaggt ctacaccatc cgggcgcacg atgccccggt gatgtgcgct cggttctcgc
    11161 ctgatgggat ggccatagcc acctgtgggc aggacggcac gatcaagata tgggaggctc
    11221 agacggacac gttgccgcga ccagcatcga gaacaggtgc tgagtagagc tcgggtaccg
    11281 gaaaccacga acggggcgag agtcggcggc ttgcaggaat gcgtttttga tcggtcgcca
    11341 gatgaccgga cggagtcaca gagatcccgc gtaaaaagcc gggtgcccgg aagcatggcc
    11401 gagtcttcaa gaggaggcta ccagactcag gcaatccttc cgggcaccct gggctgtttc
    11461 gtttctggta gtgtcatgaa cgaaaaagac cgccggatct aacgccaaga gagtgttttt
    11521 tttctgaggc caccgctgga ggcgaatacg atctggcgtg gctaggtggc cgtccgataa
    11581 cgggtatggc cgcaagttga ccagatcgaa gcgataaagt attattagta cagtggttat
    11641 ctcaatcttt gttccctcaa gtacgggttg ggattcctca tggaagaggc gaagttcagg
    11701 aagaccatgg ctgaggtgca agctggcaag gtggcagcca tggactccct gctgctggcc
    11761 agttacgatc cacttcgggc agctattgcc caacaaatga gcgatgtgtt gaggaaagcc
    11821 cagctcgagc ccgaggatgt catccaggag acctatgcgg ccgcctgggc cagcctgcct
    11881 aagagtcagt tcgagagctt cgaggcgttt ctcggctggc tgcgtcaaat cgcggaaaac
    11941 aagctgatcg acatgcaccg ggcaatcgtc gcggacaaac gtgacgtgcg acggcagggg
    12001 gccgcatggg gcgtcaagag cgggtcgtac gtcgacctgt tggatcaggt gacctcgccc
    12061 atggcgaccc ccagccgcgg ggcggcccgc aacgaggcac tagccgtttt gatggttcgg
    12121 ctccaacaac tgcctgagga ctatcgtcag gtcattcatt ggcggctgat cgaaggccta
    12181 cccgtggcgg aggtggctgg tcgtcttggc cgcagcgagg cggccgttca catgctcttc
    12241 caccgcgcct tgaaacaact ccgcgaactg atgggtgcct cttcggacta cctgagccaa
    12301 gcctgactga tcgagtcgcc cagcgacgcg ttgttcagtc ttctgtattg aacaccgcga
    12361 ggataagcct gggtcatcag acgactctgg cgggacacat ccataagcct tgcctgcggg
    12421 ggcgatgcct cgctcacact gccacactgc ctgcaaagag gtcacgattg aacgtagtgt
    12481 ctcaggccga taccatgccc gtgcaacatc tcggtcgcga gcacgttgcg atcaattcca
    12541 tggaggccgc cgtgaagtgc ccgttctgtg ccgaagagat ccaggatgcc gccatactgt
    12601 gtcggttttg tggagcggtc aagcaagacg ggcagtggta tccgcccgct cggcccacgg
    12661 tggaggcccc ccgacgcaag gggtccttca caatcaaatc ggccggggtg ctgttcatca
    12721 tttctggggc atatacgctg attaccgcga cctctgaagt gcccctgttc ggcgctatgc
    12781 gagggggcct ggtcgcgctg gcctacaacc tgggctttgc agctctgttc ctgggtatcg
    12841 gcatcggatt ggtcatcggc aagccttggg gttacggcct gatactgagc ggcaccgccc
    12901 tgtacagcgt cgacaagatt ctcctcatgt tggacaagcc ggcgcaggag gcctacttcg
    12961 ccgcagagag cagcgtgcaa tcgctagctg ctttcatcgg cctgcaggag gctgatcttc
    13021 aggcagtgtg gagccaagcg atcatcatgg gctgcctgat cagcctgggc tgctggtggg
    13081 gtttcgcatt gtacatctac gtcaggcggg actactttcg gcagagcgcg ccgcgagcgg
    13141 cggtcggacg gtgaaagggc tcgtctcttg ggccgaacgg tcctgtcgtc ttgaagacct
    13201 gtcgtgactg cggttgatca ccgtggctgt gaggagacgg aatgactcgt ctcttaagag
    13261 gtcagggcgc tctcaccctc ctccagatcc tcgcgttgca gcggcacgtt ctcgagaagg
    13321 atctcttgcc cccagatccg gtcatagagt gtcgccttct ctgccagctc aacagatgga
    13381 gtcaggatca cgtcgagtgt tgtgacgtgc ggcgcgggcc gggctatctc gcctcccaaa
    13441 ccgccatagt caaaccggcc cgtcgggcca acgcagaagc tacctcgcca gacctcctgg
    13501 ccggcgagtt ttacgacgac gaccgacgcg attgagatcg ggagcccctg cccatggatt
    13561 gtcagctcca gcgtggattt cgtttccgat cgtgcgaaca gtctggcggt agccgagagt
    13621 tggccaagcg ccatcggatt cagcttctca tcgatcacca gatcgacggc cgggtggtcg
    13681 ggtgcaacga tttcgaccgg aacgg
//
LOCUS       DAOTPW010000156        13435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4470_length_13435_cov_5.161584, whole genome shotgun sequence.
ACCESSION   DAOTPW010000156 DAOTPW010000000
VERSION     DAOTPW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13435
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4470_length_13435_cov_5.161584"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1098..2273
                     /locus_tag="P5316_14190"
     CDS             1098..2273
                     /locus_tag="P5316_14190"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRR86154.1"
                     /translation="MSTSRMLIGFCVLAAGLLGGTAVAVVPADFDGDGDVDSVDLDLF
                     EDCATSPGVPWAIGCDDKDLDADGDVDQADFGIFQRSLGSGVPMVLIPGGEFVMGDSF
                     DEGNADELPRHVVYVDAFYMDRYEVTNQQYADALNWANSQGNLITVTGGVVYKYGGDA
                     GYPYCDTTASSSGSRITWDGMTFGVTPGRENHPVVRVSWYGAAAYANWRSAMQGKAPA
                     YDLSTWNCEWNGGYRLPTEAEWEKAARGGAAETRFSWSDSNDIRHSRANYYSDWSDGV
                     PYYPYDKSPTQGYHPAFDTGAPPYTSPVGYFAANDYDLCDMVGNVWEWCNDWSSGTYY
                     GSSPYSNPTGPSSGTYRVLRGGAWDYDAFHCRVACRYGFGPAYRSFYIGFRLVLDSP"
     gene            complement(2561..2980)
                     /locus_tag="P5316_14195"
     CDS             complement(2561..2980)
                     /locus_tag="P5316_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRR86155.1"
                     /translation="MKSHRKELWFNTPRRRDYINITDQVSEAVRESGVREGLCLVNAM
                     HITASVYINDAESGLIADYDDWLEKLAPHEPVSQYRHNRTGEDNGDAHLKRQIMGREV
                     VVAITAGQLDLGPWEQIHYAEFDGRRKKRVLVKIIGD"
     gene            3317..3973
                     /gene="tmk"
                     /locus_tag="P5316_14200"
     CDS             3317..3973
                     /gene="tmk"
                     /locus_tag="P5316_14200"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRR86156.1"
                     /translation="MVLKEKLAGRFIVFDGPDGSGKGTQIELLTRKLVGEGLPVVCTR
                     DPGGTAIGDQIREILLHRNLLTAMDVRCETLLFMASRAQLVGEVIGPAVREGKTVLCD
                     RFVSATCAYQGAAGYDVRKIIELGRFAVGDLWPQLTFVLDLPADEGLKRVGRRQDKTT
                     GARHDAMEARPIDFHRRVREIFLKLPGLYPGRVEIVDGGGSPDDVHQRITEVLKRVVF
                     "
     gene            3960..5168
                     /locus_tag="P5316_14205"
     CDS             3960..5168
                     /locus_tag="P5316_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HRR86157.1"
                     /translation="MSFFDVRHQNRAHRVIQRALDSRRMPHAYLFAGPEGVGKEMLAM
                     RLAKLLLCGSPRRVPMPEEIFAEELDGREDMPTQSRGHGTREAVDACGQCQDCILVDA
                     GTHPDLFLIYRRLNKHHPDGTIRKQKALVLGVEIIRYFLIEPAGRKPSRGRAKVFVVR
                     EAERLNESAQNALLKTLEEPPPDTFIILLTDALDRMLPTTRSRCQQVLFQSLPGEFVA
                     DRLKALRPKAGAEQVTYAARHAGGSLGGALRMIDDGLFELKRSWGDRLVKLRNDAGIA
                     PNELAKPFVEDAGLLARCAAERDPEMSDTDASRVGLQMLLAALADFYMDALRRRCGAA
                     MVPVNEDQPAVIDCLSDMSERALRGCLKHLADADSSLGRNANIELTIEAMFIGLSQGR
                     TLAQAGSRAH"
     gene            5627..5980
                     /gene="ssrA"
                     /locus_tag="P5316_14210"
     tmRNA           5627..5980
                     /gene="ssrA"
                     /locus_tag="P5316_14210"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6021..6407
                     /locus_tag="P5316_14215"
     CDS             6021..6407
                     /locus_tag="P5316_14215"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRR86158.1"
                     /translation="MPCVARAKLERSRAGTGRIQAKQGGHKRSSGKAGRPSRYAGAST
                     DWVCMKYVYLIESVSHPGKRCVGSSTDFRQRLAEHNAGKSPHTSEYRPWRAVVVLRFH
                     DDRKAEAFEEYVKQGSGHAFAKRHLW"
     gene            6537..8618
                     /locus_tag="P5316_14220"
     CDS             6537..8618
                     /locus_tag="P5316_14220"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRR86159.1"
                     /translation="MRRIVLSTTVFLWAAGACAAQSAAHSDSDPRWSDWPDTDTVFKP
                     TKYGSLDEWKKRREWLRGQVRFAAGLDPEPPRTPLNAKVFGKIEHDDYTIEKAYFESM
                     PGVLVCGNLYRPKNASGKCPAVACPHGHWDKGRITHEERGSIPARCITLARLGAVVFA
                     YDMVGYNDSGKQFEHRAAVWNEPSNALWGFSILGLQTWNSIRVIDFLQSLPDVDPQRI
                     GVTGASGGGTQTFILSAIDDRVSVAAPVNMISSTMQGGCICENAPLLRIGTDNMDIGA
                     LFAPKPLLMVSATGDWTKLTPQVEFPMIRHIYELYGAADRVVNVHVDAEHNYNKSSRE
                     AMYRFFGKHLLGRPDADSLKEGDIRVDKDEDMLVFAKDAPPANTLTFDEFRQHWKDLC
                     RRQIEAMRPESAESAQKLGDFVRRALGYMVGSRFPKAGEAVRKELSSTTDQLWPCKEC
                     VYVRDSRPVRVTEVSSMMASDGRGHRVTIIVPAEGLAVLEGMKTAKAGDGSQTLELIR
                     RRANLSDRVLVVEPFGLRKPATQPAPGRGATKFFTCFNRTDPAEAVYDIVTVLGMVMN
                     EDRTRRVTLVGFGKMGPLCLVARAMVPDEPVEEKNVACIVDMNEFDEGADDAYLKHLD
                     IPGIRRIGGLQAVSAAAATGPMYLFNTGGKFDGTWTQKAGEVHKASVRVEREAVGFEE
                     ISTAINKLSEP"
     gene            9021..10124
                     /locus_tag="P5316_14225"
     CDS             9021..10124
                     /locus_tag="P5316_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86160.1"
                     /translation="MNDYHKRVLIGVELEAYSIGSADYQIGRRLSRPRPGLSEDGERF
                     SRDTSIGSEYNSRPFETVREASFLLKSGLRKYIRKLYRSHHPDRKHTVPLLVGGWSDR
                     FAGAHLHISLAGRTLERREACSLAWHIHDHIPFLIAIGSNSPIWAKQITTQASNRFLR
                     ATDIYFRPVMRGELTSDNTHEMVFSPGRKRKPPTLELRVLDSNLPQYVVVALTLVKAI
                     ALRWLKHKGATNRIRHSEYIWARLDAGVRGIRCTLPWQGEWILARQYLDRFLWEYREE
                     LQQMDVPPEVYDVLRLIKRGYTGARLIHEAALIARREHPQTWQRRFAKRYSEGLEDLL
                     SGNSLWDFAAALQVNLPDVSNVWLGRRNAPIGG"
     gene            complement(10687..12381)
                     /locus_tag="P5316_14230"
     CDS             complement(10687..12381)
                     /locus_tag="P5316_14230"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRR86161.1"
                     /translation="MDSDLGIAQSAKLLPIHEITARLGLLDEEVEPFGRYKAKVRLSA
                     IERLGEEQIGKYVVVTGITPTPLGEGKTVTTVGLAQALARIGKRAACCIRQPSMGPVF
                     GIKGGAAGGGYSQVLPMEDLNLHLTGDMHAVNSAHALLSALIDTTMVKGNPGRLDPMK
                     VVWRRCVDMNDAALRDIVVGLGGHGFPRETGFDLTSASEVMAVLALARSLKDLRHRLG
                     RIVIGYTNQDIPVFAEAIRAAGAMTALLRDAIMPNVLQTIEHVPAFVHAGPFANIAHG
                     NSSILADEIALRCVDYVITEAGFGADMGFEKFCNIKCRVSGRAPDAAVLVVTVRALKA
                     HSGQYRVVPGRPIDQTIFRPNVENLRAGLPNMEKHLENVGKFNLPCVVAINRFPTDSD
                     EEIAAIAEAARQAGAFEVAVSEVHARGGGGGQALAEAVVKAANAPGRAPFRMLYEDNW
                     PVKKKIETIVKEIYGGEGVDYEPTANAAIESLTRLGFGNLPVCMAKTQYSLSHDPTRL
                     GRPTGFRVPVRDVRLAGGAGFLTPLVGTIQTMPAFGRHPAAMDIDVDEDGRITGLF"
     gene            complement(12388..>13435)
                     /locus_tag="P5316_14235"
     CDS             complement(12388..>13435)
                     /locus_tag="P5316_14235"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86162.1"
                     /translation="LDFPPIMSKQVRLSILPSRSRLLLLPVAGLVGVMIVAGLSGCSA
                     PEEDIPPAVVKPVTAYGLSLDTTATPQQVAFVLLRSIADDVRAAQAQDAKRQKEALQL
                     TYALAAYSTIAPRIGESAAKPADAARSKARDKRLYSFVKDWAPIAAHYIRSFDTDVEA
                     AGRKMRIHSQTASAVHVLYDVCHDPAETDPARQQKATIDIELTMEPEGALSYWRVAKV
                     TFAQPTIACSVTKLSSSCVRGTDAKSEGFTVRNSGGGVLKFSLSADQTWLFLSPAGGT
                     SSGEAVTITINYATSWLEPGAHHATISIVDPAATNNPQKIVVALSVEASASPMGGTET
                     MPAAASAPSSEPPG"
BASE COUNT         2458 a         4049 c         4261 g         2667 t
ORIGIN      
        1 gagttgtatg agggaagccg aagaacaaat gcccggtaga accgcctccc gctgcctgga
       61 tcctccagtt gcgtgctacc cgaccaagga caatcagagg gagccctgga gagggccttc
      121 ttgccgcgtt tcttggcttc aaccgaaaca ctacccccca gtgtatttca cccttcgcca
      181 caggacatgc cctcttcaat ctccgttcgc cgttcttcat aaaccagcct tgccgtgtgc
      241 caggtaggag tggacatcga accgctctcc cttttgtgct cagcctttaa tccttttcgt
      301 cgggactggt gcgcattttg cgagcatgtt tctggatcgc agatcgcttg ggttgcggcc
      361 cgcaaaaagg cggtccgcct tggctttcct tccttctccc cgctcggttc ccgccggttt
      421 ctccggagca ccccgcatgt tatgccggca aaatacccaa aggagatcct gcgcgcgggt
      481 cccctggccc tacggccttc aattaaactg atttcccggc ggctcaagga gcccaaagca
      541 tgccccaggc aactcggaca acggccgctt tcgacatcag taaccacgct gcgaaaaagc
      601 tgttacatgc aatacccgtc actcgtaccg cgtcccgcta ccggcgaaaa ctgtcttggc
      661 gccaaacgaa cccaggtttc agttccacgt taatccgccc accatccgca acctggactg
      721 tggctcacct ttggcctcca gggccgcatg aacatggtcg cacacagcgc ggtctgcaat
      781 ctcattcgcc tgcttatgcg gcttgctgcc tgcgagcagg gggccggcgt gcggcggttg
      841 agccctgccg gcgagcttta tcctcacgtt ctccatccgg ctttggcaca tcattcgctg
      901 cttcaccttc aagccttcgt ttcttttgtg atcccctttc gcagcccgca atccgcctgg
      961 attctcccaa ttccgaaaat agcttgccgt gttgtcggaa aatgtggtac actaccaccg
     1021 cgtcagatga ccgatcgcat catttccgcg gaggatcaga catccccgca agaccaaaca
     1081 agtcgaggag aagaagcatg agcacgtctc gaatgctgat agggttttgc gttttggccg
     1141 ccggcctgct cggcggtacg gccgtggctg tggttccggc agacttcgac ggcgacggcg
     1201 atgtggattc cgtggatctt gacctttttg aggactgcgc tacaagcccc ggggtgcctt
     1261 gggcaatcgg gtgtgacgac aaggatctgg atgccgatgg ggatgtggac caggcagact
     1321 ttggcatctt ccagcggtcc ttgggttcgg gcgtgcctat ggtcttgatc cctggcggcg
     1381 agttcgtgat gggcgattcg ttcgatgagg gcaatgccga cgagctgcct cggcatgtgg
     1441 tctatgtcga cgctttttat atggatcgct acgaggtcac caatcaacag tacgctgatg
     1501 ccctcaactg ggcgaacagc caaggcaacc tcatcaccgt gaccggcggc gtcgtctaca
     1561 agtatggcgg cgatgccggc tacccgtatt gcgataccac cgccagctcc tccggcagcc
     1621 ggattacctg ggacggcatg acgttcggcg tgacgcctgg aagagaaaac cacccagtag
     1681 tcagggtttc ctggtatggt gcggcggcct atgccaactg gcgaagcgcg atgcagggca
     1741 aggcgccggc gtacgacctg tccacgtgga actgcgagtg gaacggcggt taccgacttc
     1801 cgaccgaggc cgagtgggag aaagcggctc gcggcggcgc ggccgagact cggttttcat
     1861 ggagcgacag taacgacatc cggcactccc gggcaaacta ttacagtgat tggtccgacg
     1921 gcgttcccta ctacccctac gacaaaagcc ccactcaggg gtatcacccg gcctttgata
     1981 cgggcgcgcc tccgtacacc agcccggtag gctacttcgc ggccaatgat tacgacctct
     2041 gcgacatggt gggcaacgtc tgggagtggt gtaatgactg gagcagcgga acgtactacg
     2101 gcagcagtcc gtactccaat ccgaccggtc caagcagcgg cacgtaccgt gttcttcgcg
     2161 ggggagcttg ggactacgac gcatttcact gccgggttgc gtgccgctac ggcttcggtc
     2221 cggcctaccg aagcttctac atcggcttcc gcctggtcct ggattctccg tgacgctttg
     2281 cgccccgttc tcttgtgcgt cttgtctcgc tcgcaagcgg gggaccaccg ataagcccgg
     2341 tgtgcgtcac ccggcggcgt cagcgatccg acgaccggtt tgccgaacgg ggcgcggcgg
     2401 tacgcggagc ggcttgtctc gcttcccgac ggcggccggg ttgtgctcgc ttcgcctgtg
     2461 tccgaccgag cccgatcccg gcaaccctcc cggtccacgc tccgagagat ttcctgcctt
     2521 tttacacttt tgtgagatac gacgggcaac tgggggcttc tcagtcccca atgatcttta
     2581 ccagcacgcg cttctttctc cgtccgtcga actcggcgta gtggatctgt tcccacgggc
     2641 cgaggtcaag ctgcccggcg gtgatggcca cgaccacctc gcggcccatg atctgacgct
     2701 tgaggtgggc gtcgccgttg tcctcgccgg tccggttgtg gcggtactgc gaaacgggct
     2761 cgtgcggggc gagcttttcc agccagtcgt cgtagtcggc aatgagtccc gactccgcgt
     2821 cgttgatgta cacgctggcc gtaatgtgca tggcgttgac caggcagagg ccttcgcgga
     2881 cgccgctttc ccggacggcc tcggacacct ggtcggtgat gttgatgtag tcgcggcgac
     2941 ggggagtgtt gaaccacaat tccttacgat gagatttcat tgctgggaat cctttctgga
     3001 tgttggctat tagctattac aaccttacgg ccagatatct gtgcgacttg cgcgcatgct
     3061 tctggatcgc agaacgtttg ggttgcggcc cgcaaaaagg cggtccgcct tagatctcag
     3121 ttattgggtc ttggctgtta gcggttggtg cttgcccacg gtcggtgccc tgttcatcct
     3181 tcatcctttc gccttcagcc tttcgtcttg ctcctcgccc ctttatttgt cccggccttc
     3241 ccgcttacac tgttgggccc gttaagtagt gtattgtccg gctcggggcg ggaacatcaa
     3301 gagcaagagg ctgatagtgg tactcaaaga gaaactggcg ggtagattca ttgtttttga
     3361 cggtcctgac ggctcgggca agggcacgca aatcgagttg ctcacccgca agctggtggg
     3421 cgagggtctg cccgttgttt gcacccgcga tccgggcggc accgccatcg gcgatcagat
     3481 ccgcgagatc ctgctgcaca ggaacctcct gaccgcgatg gatgtccgct gtgagacact
     3541 cctgtttatg gcatcgcggg cccagcttgt aggtgaggtg atcggcccgg ccgtccgtga
     3601 gggcaagacc gtcctttgcg accggttcgt atcggcaacg tgcgcgtatc agggagcggc
     3661 cgggtacgac gtcaggaaga tcattgagct gggacgcttc gcggtggggg acctctggcc
     3721 gcagctcacc ttcgttcttg atctcccggc cgatgaaggt ctcaagcggg tcgggcggcg
     3781 gcaggacaag acgaccggtg ctcgccacga cgcgatggag gcccggccga tcgattttca
     3841 ccgacgtgtc cgcgagatct tcctgaaact gcccgggctt tatcccggcc gcgtcgagat
     3901 cgtggacggc gggggctcac cggacgacgt tcaccagcgc atcacggagg tgttgaaacg
     3961 tgtcgttttt tgacgttcga caccagaacc gggcccaccg cgttattcag cgggcgctgg
     4021 actcccggcg catgccacac gcgtacctgt ttgccgggcc cgagggcgtg ggcaaggaaa
     4081 tgctcgccat gcgcctggcg aagctgctgt tgtgcggatc gcccaggcgg gtgccgatgc
     4141 cggaagagat ctttgctgag gagctcgatg gccgagaaga catgcccacg caaagccgtg
     4201 gacatggcac ccgtgaagca gtggacgcct gcgggcaatg ccaggactgt atcctcgtcg
     4261 atgccgggac ccaccccgac ttattcctga tctatcgccg gctcaacaag caccatcccg
     4321 acggcacaat ccggaagcaa aaggccctcg ttctgggcgt cgaaatcatc cgatatttct
     4381 tgattgagcc tgcgggcaga aaaccaagcc gcgggcgtgc caaagtgttc gtggttcgcg
     4441 aggcggagcg cctgaacgag tcggcccaga acgcgctgct caagacgctt gaagagcccc
     4501 cgccggatac gttcatcatt cttttgacgg atgccctcga ccggatgctc ccaacgacgc
     4561 ggtcgagatg ccagcaggta ctctttcagt cgctgcccgg cgagtttgtg gccgatcgtc
     4621 tgaaagctct tcggcccaag gcgggggccg aacaagtgac ctacgccgcc cgccacgcgg
     4681 ggggcagcct cgggggggcg ctcaggatga tcgacgacgg cttgtttgag ctgaaacgct
     4741 cctggggcga ccgactcgtc aagttgcgga acgacgccgg gatcgccccc aacgagttgg
     4801 ccaaaccttt cgtggaagat gccggcttgc tggccaggtg cgcggcggag cgcgaccccg
     4861 aaatgtctga tacggacgcg tctcgcgtcg ggttgcagat gctcctggca gccctggcgg
     4921 atttctacat ggatgccttg cggcggcgtt gcggtgcggc tatggtgccg gtcaacgagg
     4981 atcagccggc tgtgatcgac tgcttgtccg acatgagcga gcgggccttg cgcggttgcc
     5041 tcaagcatct ggcggacgcc gattcgagtc ttggccgcaa cgcgaacatc gagttgacga
     5101 tcgaggccat gttcatcggt ctttcgcagg gccgaacgct cgcacaggcc ggcagccggg
     5161 ctcattgaca gggaggttgc ggatatgatg ctgatccagt ggctgttgca attgcttgtg
     5221 atgttggcgt atatgttggt cggtggggcg tgagcaaaga gacccgcccc gctgatagtg
     5281 cacggaggta caaggcgatg ttcatcgcgc ttttccggtg cgtttggtcg cacggatgaa
     5341 ccgccaggtt tcttaacgtg agggcttcgt gatgccttgg tttgaatggc tcgttcagtt
     5401 gttgacgttg ctgctgagta tcttcggcgg cgggagctgc gggagctgcg gatgacgccg
     5461 cggatctgtc cgtaacccat cgggccggca ggggcatgtg aggtgtctct gccggcccga
     5521 tagcgtctca gcgcacagac gccgataatg ttcgctcgaa gcacaaagat ctgcttgcgt
     5581 gggcggtccg cgagggacta taatagatga cacaagtgcc ctttttgggg gcgatttggg
     5641 ctcgaccggg caaatgaggt cgaggcggcg tgtccaggtt gtcaggaggc ctggctaaac
     5701 acctggcaaa agaacacaaa tgccaactca cagttgcgca tggccgccta aatgaggcga
     5761 cccgtcttgt caacgacgcc tgctagttga ccggacgcag acagcaggca cgcccgcgag
     5821 ggtaggcgac gtgacacgcg ggtaagacta ccacgacgct gggacggccg aagcctgccg
     5881 ctgggcgacg ggcgtctgag aacaaatcag acggctacac acgtagaaac ctcggtcgaa
     5941 ttgtcacggg acgcgggttc gattcccgcc gcctccatcc tgcatagctg cggcccggtt
     6001 ccggtccgga gctatgcagg atgccctgcg tagcgcgagc gaagctcgag cgaagcaggg
     6061 cgggcacagg cagaatccag gcgaagcagg gcgggcacaa gcggagctcg ggcaaagcag
     6121 ggcgaccctc gcgctatgca ggggcttcga ccgattgggt gtgcatgaag tacgtgtacc
     6181 tgatcgagag cgtctcacat cccgggaagc gctgcgtggg ttcgagcacc gacttcaggc
     6241 aacgcctggc tgagcataac gccgggaagt ctccgcatac ctctgagtac agaccctggc
     6301 gggcggtggt tgtccttcga ttccacgacg ataggaaggc agaagccttc gaggagtatg
     6361 tcaagcaggg ctctggccac gcgttcgcca agcggcattt gtggtaaaac gaggtccgct
     6421 tccgtccggg cgttcgcgag gaggcacctc cgaccatctg tcctcctcgc gatctccggt
     6481 tacaatctcc ccagccggcg cgtgcccgtt tttgtcaact cactggaggt tctgcgatgc
     6541 gacgaatcgt gctttcgacg acggtgttct tgtgggcggc tggtgcctgt gcggctcaga
     6601 gcgcggctca cagtgattca gacccccgat ggtccgattg gccggatacc gacacggtgt
     6661 tcaagccgac aaagtacggc tcgctggacg agtggaaaaa acggcgggag tggctgcgcg
     6721 ggcaggtgcg gttcgctgcc gggttggatc cggagccgcc gcggacgccg ctgaacgcca
     6781 aggtcttcgg caagatcgag cacgacgact acaccatcga gaaagcgtac ttcgagagca
     6841 tgccgggggt gctggtgtgc ggcaatcttt accggccgaa gaacgcttcc ggcaagtgcc
     6901 cggcggtggc ctgtccccac ggacactggg acaagggacg catcacccac gaggaacgcg
     6961 gatcgattcc cgcacgatgc atcaccctcg ctagacttgg cgcggtcgta tttgcctatg
     7021 acatggtcgg gtacaacgac agcggcaagc agttcgagca tcgagcggcc gtctggaatg
     7081 aaccctcgaa cgcgttgtgg ggcttcagca ttctcggttt gcagacctgg aacagcatac
     7141 gggtgatcga tttcctgcag tcgcttccgg atgtcgatcc gcagcggatc ggcgtaaccg
     7201 gagcctcggg cggcgggacg cagacgttca tcctgtcggc gattgatgat cgggtttccg
     7261 tggccgcacc cgtgaacatg atctccagca ccatgcaggg tggttgcatc tgcgagaatg
     7321 cgccgctgct tcggattggg accgacaaca tggatatcgg ggcgctcttt gcccccaagc
     7381 ccctgctgat ggtcagcgcc accggcgact ggaccaagct cacgccgcag gtcgagttcc
     7441 cgatgatccg ccacatctac gagttgtacg gcgccgccga tcgggtggtc aatgtccacg
     7501 tcgatgccga gcacaactac aacaagtcca gccgcgaggc gatgtacagg tttttcggca
     7561 agcacctgct cggccggccg gacgcggatt ctttgaagga aggcgacatc cgggtggaca
     7621 aggacgagga catgctggtc tttgccaagg atgccccgcc cgcgaacacg ttgacgtttg
     7681 atgagtttcg gcagcattgg aaggacctgt gccgccggca gatcgaggcg atgaggccgg
     7741 agagtgctga aagtgcccaa aaactcggcg acttcgtgcg acgtgcattg ggttacatgg
     7801 tcggcagtcg cttcccgaag gctggtgagg ccgttcgcaa ggagctcagc tccaccacag
     7861 accagttgtg gccctgcaag gagtgcgtct acgttcggga cagccggccg gtgcgtgtaa
     7921 cagaagtgag ctcgatgatg gcgtcggatg gccgtgggca tcgtgtaacg atcatcgtgc
     7981 cggccgaggg gctcgcggtg ttggagggga tgaagactgc aaaggccggc gacggatcac
     8041 aaacactcga gctgatacgg cgccgggcca acctgtcgga ccgcgtgctg gtggttgagc
     8101 ctttcggtct ccgcaagccg gccacccagc ccgctcccgg ccgtggggcg acgaagttct
     8161 tcacctgctt caaccggacg gacccggccg aggcggtgta cgacattgtg accgtgctgg
     8221 gaatggtcat gaatgaggac cgtaccagac gggtgactct ggtcgggttc ggcaagatgg
     8281 gaccgttatg tctcgtcgcc agggcgatgg tcccggacga gcccgtcgag gagaagaacg
     8341 tggcctgcat cgttgatatg aacgagttcg acgagggagc cgacgacgcg tatctcaagc
     8401 atctcgatat cccaggcatc aggcgaatcg gcggactgca ggcggtttcg gcggcggccg
     8461 cgaccggacc gatgtatctg ttcaataccg gtggcaagtt cgatggaacc tggacgcaga
     8521 aggccggcga ggttcataag gcgagtgttc gcgtggagcg ggaggcggtc ggcttcgagg
     8581 aaatcagcac cgcgatcaac aaactgtccg aaccgtgatc ctggtacccc tcctgcagca
     8641 gtgcccggca ccgtcgcttg gacccgcgcc accggcgtgt cgcatcagcc tccgctgcgt
     8701 gattgaggtg cgccgttgcc gtcggccagt tccggcacaa acggcctgaa aggctgagcc
     8761 tgatatactt cgcgcaattg ttcggtggcc atgagtctgc tgccgcggtt cgatcctgcc
     8821 gcgtccagtc aacggatgtc aaccaattga ctctccgctt tgtatcgcct caagaagagc
     8881 taagcaatga cggaaacaga acttccgttt gtcgggggat tcgcgaccga ttgctcttgc
     8941 cagattctcg cacttggtgc tatgctgaaa ccccaatcgc cgcgtgaagt accgcggagc
     9001 cgccatgaag agccattacc ttgaacgatt atcataaacg ggtcctcatc ggggtcgaat
     9061 tggaagccta cagcatcgga tcggcggact atcagatcgg tcgccgtctg tcccggccac
     9121 gaccgggctt gtccgaggat ggggagcgct tcagccgcga tacgtcgatc ggcagcgaat
     9181 acaacagccg gccattcgag accgtgcgcg aggcctcctt cctgttgaag tcggggctgc
     9241 gcaagtacat acggaagctg taccgaagcc accatcccga ccgcaagcac acggtgcccc
     9301 tgctggtcgg cggctggagc gaccgtttcg cgggtgccca cctccatatc tcgctcgcgg
     9361 ggcggacact cgagcgccgc gaagcctgtt cactggcttg gcacattcac gaccacattc
     9421 ctttcctgat cgcgatcggc tccaattcgc ccatctgggc caagcagatt accacacagg
     9481 cttccaaccg ctttttgcgg gcaacagata tctactttcg cccggtcatg cgcggcgagt
     9541 tgacctcgga caatactcat gaaatggttt tcagcccggg gcgcaagcgc aaaccgccca
     9601 ccctggagct tcgtgtcctc gactccaatc tgccgcagta cgtcgtcgtg gcattgaccc
     9661 tcgttaaggc catcgcgctg cgctggttga aacataaggg ggccaccaat cgtatccgtc
     9721 actccgagta catctgggct cggctcgacg ccggcgttcg tggaatacgc tgcacgcttc
     9781 cgtggcaagg tgagtggata cttgcccggc aatacctcga ccggtttctg tgggagtatc
     9841 gcgaggagct gcagcagatg gacgttcctc cggaggtcta cgatgtgctc cgccttatca
     9901 agcgagggta caccggtgcg cgcctgattc acgaggcggc cctgattgcc aggcgcgagc
     9961 acccccagac gtggcagcgg cgcttcgcca agcgctacag cgaagggctc gaggacttgt
    10021 tgagcggcaa ctctctctgg gacttcgcgg ccgccctgca ggtcaacctt cccgacgtca
    10081 gtaacgtctg gcttgggcgt cgcaatgcgc cgataggggg gtagcagggc ggcccgctgg
    10141 cgccgtgaga tgatcacggc ctttgcccgc aacccaagtg aatgacgaat gacgaaaccc
    10201 gaatgacgaa tgaatgacga aggcagggtg gcccattctc gccgcagcga gggtggggtc
    10261 tcgatgatgt gtggagaagg gtgtatcgag aggaacgtca cccccgttgc tgagttcggc
    10321 accaccagta gggccgacgc cctcgtcggc cattgtccgc gaatagccgg atacgacttc
    10381 gtggccgcga agaggcacga aaagcacgga gaagactgcc cggagactgt ggcggccgcg
    10441 cgccggcgtc ctctgcggtc aagagggttt tatcccccgc cccttgtcat ggcgagcgat
    10501 agggcagccg catgcgttcc tgccgaacat ggccgcgcaa aggccgtagc catggcactc
    10561 ggcacccggc atggcaagga tcaggcactc gccaagagtt aacagcgtcc tcttggtatc
    10621 gttgcccccg gaacggcagg tctccgccgc ctcggcggct ccgaccgtgc cctacctcgt
    10681 gaacactcag aacagtccgg tgatccgtcc gtcttcgtcg acgtcaatgt ccatcgcggc
    10741 cgggtggcgc ccgaaggcag gcatggtctg aatcgtgccg accagcggcg tgaggaagcc
    10801 cgcgccgccg gccagacgaa cgtcgcggac cggtacgcgg aacccggtgg ggcggccgag
    10861 gcgagtggga tcgtgtgaaa gcgaatactg ggtcttggcc atgcagaccg gcaggttgcc
    10921 gaagcccagc cgtgtcagcg actcgatggc cgcgttggcc gttggttcat agtcgacccc
    10981 ctctccgccg tagatctcct tgacgatggt ttcgatcttc ttcttgacgg gccagttgtc
    11041 ctcatagagc atgcggaagg gagctctgcc aggtgcgttt gcagccttga ccaccgcctc
    11101 agccaaggcc tgcccaccgc cgccgccgcg ggcatggact tcgctgacgg ccacttcgaa
    11161 agccccggcc tggcgcgcgg cctccgcgat ggccgcgatc tcctcgtcgc tgtcggtcgg
    11221 aaagcggttg attgccacga cgcacggaag attgaacttg cccacgttct cgaggtgctt
    11281 ttccatattg ggcagaccgg cccgtaggtt ttcgacgttt ggccggaaga tggtctgatc
    11341 gattggacgg ccgggcacca cgcggtactg gccgctgtgg gccttcagtg cccggacggt
    11401 aaccaccagc accgccgcgt ccggcgctcg gcccgagacc cgacacttga tgttgcagaa
    11461 cttctcaaaa cccatgtccg cgccgaaacc tgcttcggtg atgacgtagt cgacgcaacg
    11521 aagggcgatt tcgtcggcca ggatcgagct gttgccgtgg gcgatattgg cgaacggtcc
    11581 ggcatgcacg aacgcaggaa cgtgctcgat ggtttgaagg acgttgggca tgatcgcgtc
    11641 tctgagcaag gcggtcatgg ccccggcggc gcggatcgcc tcggcaaaga cggggatgtc
    11701 ctggttggtg tagccgatga cgatccgacc gagacggtga cgcaggtctt tgagtgaacg
    11761 ggccagggcc agcacggcca tcacctcact ggcgctggtc agatcaaagc cggtttcccg
    11821 cggaaaaccg tggcccccaa gccccaccac gatgtcccgt agtgccgcat cgttcatgtc
    11881 cacgcagcgg cgccacacca ctttcatcgg atccaggcgg ccggggttgc ccttcaccat
    11941 cgtcgtgtcg atcagggcgc tgagcagggc gtgggcgctg ttgactgcgt gcatgtcgcc
    12001 ggtgagatgg aggttcaggt cctccatcgg caggacctgc gaatacccgc cgccggcggc
    12061 gcctcctttg attccaaaga ccggccccat ggatggttgt cggatgcagc acgcggcgcg
    12121 ctttccgatg cgcgccaagg cctgggccag cccgaccgtt gtgacggtct tgccttcacc
    12181 gagcggcgtg ggagtgatgc ccgtcacgac gacgtacttg ccgatttgct cttctccgag
    12241 gcgctcgatc gccgacagcc ggactttggc cttgtatcgg ccgaacggtt cgacttcctc
    12301 atccagcaga ccaagccgcg cggtgatctc gtgtatcggc agcagtttgg cactctgtgc
    12361 aatccccaga tcggaatcca tggcaaatca tccaggaggt tcggaagagg gagcggaagc
    12421 tgcggccggc atggtctctg tcccgcccat cggtgatgca gaagcttcga cgctcagtgc
    12481 gacaacgatc ttctgcgggt tgttcgtcgc cgccgggtca acaatactga tcgtcgcatg
    12541 atgtgcaccc ggctccagcc acgacgtcgc gtagttgatc gtgatggtca cggcttcgcc
    12601 ggagctggtt ccgccggccg gcgaaaggaa caaccaggtc tggtctgcgc tgagcgaaaa
    12661 cttgaggaca cccccgccgc tgttacgcac cgtgaagccc tcgcttttgg cgtcggtgcc
    12721 ccggacgcag gaagaagaaa gctttgttac cgaacaagcg atcgtcggct gcgcgaaggt
    12781 gactttcgcc acccgccaat acgacaaggc gccctccggc tccattgtca gctcgatgtc
    12841 gattgtcgcc ttttgctgcc tggcggggtc ggtttcggcc gggtcgtggc acacatcgta
    12901 caggacatgg accgcgctgg cggtttggga gtggatccgc attttgcgac cggccgcctc
    12961 cacgtcggtg tcgaaactgc ggatatagtg ggcggcaatc ggggcccagt ccttgacgaa
    13021 gctgtaaagc cgcttgtccc gggctttcga tcgggcggca tccgccggtt tggccgcgga
    13081 ttcgccaatt cttggggcga tggtcgagta ggccgccagt gcgtacgtga gctgcagggc
    13141 ttccttttgc cgcttggcgt cctgcgcctg ggcggcccgc acgtcatcgg caatcgagcg
    13201 aagcagaaca aaggcaacct gctgcggtgt ggcggtggtg tcgagactca gcccataggc
    13261 cgtgacaggt ttcactacgg ccggcggaat gtcctcttcc ggagcgctac aaccggacaa
    13321 gccggccaca atcatgacgc caacgagccc cgccacgggc aggaggagca accgtgatct
    13381 cgatggcagg atcgataatc gcacttgttt actcataata ggcgggaagt ctaac
//
LOCUS       DAOTPW010000157        13318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4513_length_13318_cov_5.187665, whole genome shotgun sequence.
ACCESSION   DAOTPW010000157 DAOTPW010000000
VERSION     DAOTPW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13318
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4513_length_13318_cov_5.187665"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3250
                     /locus_tag="P5316_14240"
     CDS             <1..3250
                     /locus_tag="P5316_14240"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRR86163.1"
                     /translation="PVFATEISQIDPLPHQRIAVYEQMLKQFPLRFLLADDAGAGKTI
                     MTGLYIREMKARRLIKRVLIVAPAGLIGNWKRELATLFSLEFRIVTGADARDANPFVG
                     EESDCVIVSVDTLAGAKTFARLREPDVVPYDLVVFDEAHKLSADRGSDLRVRKTDRYR
                     LAEALAGVGGVGEAWTLPWTAHHLLLLTATPHQGKDYPYYALWRLLEPEVLATPEAFD
                     EYPPERRRAHFIRRTKEEMVHISGKPLYPKRISDTLGYDLTQGPISEQTLYDETTDYM
                     RHVYNRAKVLNRSAARLAMSVLQRRLASSTYALLRSFERRIEKLDQLIRQVQEGRLTE
                     EQLVVLQRRLAEEDDVLDTKTADEEGTENGREEHEISEERLLAGVIAASLTDLVVERE
                     QVVALRDLARKVYDQGAESKFDKLREVLTDPKFVGEKFIVFTEHRDTLDYLVQRLGGM
                     GYTGQIAQIHGGMDYIERQEQVERFRRPHNDGGARFMICTDAAAEGINLQFCWIMINF
                     DVPWNPARLEQRMGRIHRYGQKHDPVHIVNLVAPKTREGLVLQTLLKKLETIRDRLGS
                     EKVFDSIGRLFEGVSLKQYMEQAVVEGADVAAHQLEGRLTEEQVAALAARERMLYGDG
                     GDVARQLPRLRDDLEQEAYCRLLPGYVRHFIENAAPLVDIEIDGHLDGCFSLRPARKG
                     AVDPLIHALEMYPAKQRGCLTVNRPDPDNKDRAIWLHPGEPVFERFRGLVSERLGDQA
                     LRGAVFIDPTTEKPYLFHLALISVIRQADPELPDLASEETLDCQLVGVKQYEGAEVVL
                     CPVEHLLLLKGGHGLPAAAQRLAVTAGKLKEQAEAFIMERVARGLAVQRRNAMLDTLP
                     EREQFIRRGFDFQEAELAAARVKQSEKARSGNAAAIKALKEIKEQQRSLAQRRHAALA
                     TIRREPELIAPGHLTFLAHALVVPSSDPADLERHDAEVEKVAMDLARAFEEACGATVK
                     DVHTPELARAAGLTDNPGFDLLSIYPDGQRRAIEVKGRASTGDVEVSSNEWARAANLR
                     EGYWLYAVYDCATPNPRLVRVQDPFGNLLAKAKGSVLISANQITQAAKESIA"
     gene            3247..6591
                     /locus_tag="P5316_14245"
     CDS             3247..6591
                     /locus_tag="P5316_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HRR86164.1"
                     /translation="MTDFPRLIEHAFPLKQASLDSVHEKNVRHGHISTLHIWPARRPL
                     AACRAALIATLLPDPSASPKPEGMTDTQWEAEIKRRRRELCEKIGGKVVTKNEKKKMP
                     NGQTVERIKEETVGGILHWGRETENAETLEWFRQEIRKAYGGRAPKVLDPFAGGGAIP
                     LEAMRLGCEATAIDINPVAWFILKCTLEYPQKLAGQTRPLPEFILHNDAFMAEFFKKA
                     KGYGEAEVTAALKRLHKSLKKKTKDTGKGRQGALPFAPLASTEGEELLADLAWHVRAW
                     GQWVLDRARRELAQCYPTYADFEPIKKDHIAYEHQPMRLVPLKDDGTPDIDSLNAEFT
                     EEYLADKRNPRWVAKPTVAYLWARTVVCKNCRATVPLLKTRWLCKKPNKRVLLTMEPN
                     ADRTAVVFGVDASVPAKGGNAAQKREHDKRLGAGTMSRAGAQCPCCGAINTMEDIRLE
                     GKAGRLGSVMTAVVVDTPAGKEYRLPTEHELAVATPSEEQVQAAFKDVPFGVPDEPTP
                     AGGGSGAGRAFSVQGYGLMKWRDLFTPRQLMALGTFVAATRAARCDMAFPQLPPEWIE
                     AISAYLASILDFVINRNSTICTWTLTRQTTRSTFARFALPISWDFSEASVLEQGSGGY
                     SGAIDWIGRCIDHTLSFATDCPAPSVEQRSATAPSDKGYDVVLTDPPYYDAIPYSDLM
                     DYFHIWLRRTLFGISPSVDAAFAHTLSPKWDSEANDGELIDDCSRHGNDAALSKHVYE
                     DGMARVFGSCCEALKPEGRLVIVFANKQPDAWETLVSAIIRAGFVVDGSWPIQTEMGN
                     RTRALVGAALASSVWLVCKKRPATAKPGWDNKVLEEMRANIAEKLRDFWDAGIRGPDF
                     VWAATGPALEAYSKHPVVRKANEPNATMNVGEFLNHVRRMVVDYVVGQVLSGEDPETR
                     GRGDAAIGNLDEVTAYYLLHRHNFGMDEAPVGACILYATACSLSDAELEKTWDILSRT
                     GSDAAAEEDSEAEESADAAGDAEPAEDSGGGSKVKLKAWNQRKGKSMGYEAPEGKPVP
                     LIDRIHRVMHLWKEGDVQKVDEYLDQHALRRNELFKRVVQSLIELSSNSERSLLESIS
                     NHIGAKGAVRDKGPTLFADGPVDEES"
     gene            6592..8394
                     /locus_tag="P5316_14250"
     CDS             6592..8394
                     /locus_tag="P5316_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRR86165.1"
                     /translation="MSTDGYDITPDKRPIIGLVQQAYEGKACLPNFQRDFVWPREGVA
                     DLLRSVLRGYYIGALLLLRSDPQEPPFAPVFLRGAKPAHRDPRPELLILDGQQRLTAL
                     LYALTAPQLPLKDSSRPRRFFTDLQLLLEEPDNDGIVFDVAENELNGLEHEEEQYRRR
                     ILPCTTMFRLDDFMTWRDGLDDWLREQEPENHKRFREEWRTPWTKAVGAFQNFLVPLV
                     ELPQVRDSDTFAIGRVCAIFEKLNSTGVDLSVYDLLTARLYRHRIRLHQLWKETCQQH
                     KRIAAWSGGKADTNKLGVLVLRTLALLRGLEPKPRILINLKPEGFEDDWRRAAAAMER
                     AFELVEHVGPDGFGVFHQKWLPGFGLIPVLAALRAEIEERNLGEAARAELRRWYWCNV
                     FLERFSSGVESKSRKDYAEFLDHWLNDKPEPAVFAEARARIGAAGYSIRGSASYASAV
                     YCGVFCLLAIRGARDWSLGESIQLQKLNDHHVFPRAYLREHKITKKPVVNSIVNRTLI
                     SNMTNNKIKAKRPSEYLAMPEIVPPLRGDAVLEPHFLDEASRAALRATVPSLSSEEVA
                     AQYEAFCTARERTIIAEIRRVCGIDMDVEAPGGR"
     gene            8447..11785
                     /locus_tag="P5316_14255"
     CDS             8447..11785
                     /locus_tag="P5316_14255"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HRR86166.1"
                     /translation="MPWHRVVKLREDLKSGDLPLHMFAADLYEVMMGSGKRPIYEKPE
                     EFFALTFPTHNLRGLVRDVVLRLAGKNDKAVRQLELTYGGGKTHTLITMRHLVNDPDN
                     LPDLPAVKEFTETIGQTPPKARVAALCFDKLDVETGCDVRSPDGAVRRLKQPWSLLAY
                     QIAGDEGLKLLHADGKAEERTTPPAENTLSQLLSLPLKEGMGTLILLDEVLLYAKVRC
                     HTEPGFLDVLIGFFQYLSQAAAKVEKCCIVASLLSSEPKDQADALGRKIVSDMYDIFQ
                     RQREEAVQPVEKDDVAEVLRRRLFDPQSVELRGQWPQHVIAALKGISALDDQTAKQGG
                     AAEERYLKSFPFHPELTEVFYTKWSAGIERFQKTRGVLRTFALALREAEKWDESPLIG
                     PAVFLNAAKQEGLSDSARELVAVADTIVTDGQATKWTGILETELSCARQVQSESVGLK
                     LREIEQAVMATFLHSQPTGRSAKARDLMLLIGPCRPDKIELEKGLARWARSSYWLDDT
                     NLPEKDGQLPNEWRLGNRPNLNQMHAAAAAQVSDDAVKARLVEEIGRTKSLTSGASAL
                     GVRVHTLPTKPKDIEDDGLFHYAILPPSAASDSGKPSAEAKRFLDETTGAEKPRVFRN
                     AVLLLAPSRDGLNVAAARVRDHLAWEKVMDDLVPKNEDEKKQKGPVDIARLQTLKINI
                     DKAKARVPEAIRQAYCIVVTVSDKDDVQAFKITVTDEAHFETIKNDPRSRVQDKAVTA
                     EALLPNGPYNLWKGGETSRRVKDLAGAFAQLPHLPKMLKSQAIMETLVDGCVQGTFVL
                     KLTRPDRTFRTWWRTRPDDTALADPALELVLPEAAELGSIPGELLAPKALPDLWTGDE
                     VTVQAVTQYFSGNTVVQVDKGGFKEPVPVPKAATDVVNAAVAEAVANGKVWLLSGPAS
                     LLAEPIPTGVLSPASRLRVPPLPIAAAAILPENLPTAWQDGATTALAIATGLSQQAGY
                     TLPWKTVRDVIGGSLQARFIALAEDSAPWPCDLAAAQTVKIKVAAAGTGGGTGGGTGG
                     GTGGGGAPLPNVRVAAADFEPSQMQDLGDLVPALLDIKAKAKVAMKFHVRLEVGDAGS
                     RPSDDVVGDINNLLKDLGEGFRLV"
     gene            11943..13112
                     /locus_tag="P5316_14260"
     CDS             11943..13112
                     /locus_tag="P5316_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86167.1"
                     /translation="MGRQYSAKTFLRNAPNRLLQQYFQKQDLNLGLDWVRLDETEVNP
                     IFLALEALPAATRSDVDTDFALIHEMACEHGVQAIVEEGRNWGRDWAEQFAGMRNHYE
                     RAFWTFLNEPRRFHVAGCFHEMDRLGGWRRRFVGRRLEAQDDEDALAELERRVRLFYR
                     RQGRGRFCHVDYYERREPERHCYFAYPEDYASTDVGYDENGRFLHRPRRPAFEVIFVY
                     RPEEGVLELHAKGNKRQADELAEIFCTTTLGLVKLPDDNDRVPFDLAVLKNPEFPFPT
                     DPADRISAVHVRQLRFDLPEPTRGRITVSAHASDHRPRALRDVVDRAIDRARMPLEAL
                     HVSQAKMRLVFAPHNGERPKTLTFEVTYPDRCTLKDDPLDQIAKKYLSQWGIARD"
     gene            13105..>13318
                     /locus_tag="P5316_14265"
     CDS             13105..>13318
                     /locus_tag="P5316_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86168.1"
                     /translation="MTDAIRHIMHGFERGPVVAFTGQETARWPRGLLAALVDGAILHE
                     TQLADSVWCDECEEGCSITPDLHEHPG"
BASE COUNT         2568 a         4176 c         4354 g         2220 t
ORIGIN      
        1 ccccgtgttt gccaccgaga tttcgcagat cgaccccttg ccccaccagc gaatcgcggt
       61 ctacgaacaa atgctgaagc agttccccct ccggttcctg ctcgcggatg acgccggcgc
      121 gggcaagacg atcatgacgg gcctctatat tcgtgaaatg aaggcccgtc gcctgatcaa
      181 gcgcgtcctg atcgtggcgc ccgcaggact gatcggcaac tggaagcgcg agttggccac
      241 gctgttcagc cttgagttcc gcatcgtcac gggggctgat gccagagatg caaacccgtt
      301 cgtgggcgag gagagcgact gcgttatcgt cagcgtcgac acgctcgccg gcgcgaagac
      361 gtttgcacgc cttcgggaac ccgacgttgt tccgtacgat cttgttgttt tcgacgaagc
      421 gcacaagctc tccgccgatc gcggaagcga ccttcgcgtc cgcaaaacgg accgttaccg
      481 gctggccgaa gcgctggcgg gtgtgggcgg cgtcggtgaa gcctggacgt taccttggac
      541 ggcacatcac ctgttgctgc tgaccgccac gccccaccaa ggcaaagatt acccgtacta
      601 cgccctgtgg cgtctgcttg agccggaggt tcttgcgacg ccggaggcat tcgatgagta
      661 cccgcccgag cgtcgccgtg cacacttcat ccggcgcaca aaggaggaga tggttcacat
      721 cagcggcaag ccgctgtatc cgaagcgcat ctccgacact ttggggtacg acctcaccca
      781 aggtccgatc agcgaacaga cgctctacga cgagacaacc gactacatgc gccatgtcta
      841 taaccgcgcg aaggttctga atcgctccgc ggcacggctg gcgatgagcg tgttgcagcg
      901 ccgcctcgca agttccacct acgccctgct gcggtcgttc gagcgtcgaa tcgaaaagct
      961 ggaccagttg atccgccaag tccaggaggg aaggcttacc gaggagcagc ttgtcgtcct
     1021 ccagcgccgt ctcgccgagg aagacgacgt cctggatacc aaaactgctg acgaggaagg
     1081 cacggaaaac gggcgagagg agcatgaaat ctccgaggag cggctgctcg ccggcgtcat
     1141 cgccgcttcc ctgaccgacc tggtcgtcga gcgtgagcag gtcgtcgcgc tgcgcgacct
     1201 tgcccgcaag gtctatgacc agggcgcgga gtccaagttc gacaagctgc gcgaagtgct
     1261 gaccgatccg aagtttgtcg gcgagaagtt catcgtattc acggaacacc gcgatacgct
     1321 cgactacctc gtccagcgcc ttggcggcat gggctacacc ggccagatcg cccaaatcca
     1381 cggtggcatg gattacatcg aacgccagga acaggtcgaa cgattccgtc gccctcataa
     1441 tgatggtggt gcccggttca tgatctgcac cgacgcggcg gcggagggca tcaaccttca
     1501 gttctgttgg atcatgatca acttcgacgt gccctggaat ccggcccgcc tggaacagcg
     1561 catgggccgc atccaccgct acggccagaa gcacgacccc gtacacatcg tcaacctcgt
     1621 cgctcccaag acgcgcgagg gcctcgtcct tcaaacgcta ctgaagaagc tggagaccat
     1681 ccgcgatagg ctgggcagcg aaaaggtctt cgactccatt ggccgcctct tcgagggcgt
     1741 ctcgctcaaa cagtacatgg aacaggccgt tgtcgagggc gcggatgtcg ccgcgcacca
     1801 gctcgagggt cggctgaccg aggagcaggt cgcggccttg gccgcaaggg agaggatgtt
     1861 gtacggcgac ggcggcgatg tggcccgcca gcttccgcgg ttgcgcgacg acttggagca
     1921 ggaagcctat tgccggttgc tgcccggcta cgtccgccac ttcatcgaaa acgctgcccc
     1981 gctcgtggat atcgaaatcg acgggcacct ggacgggtgc ttttcactgc ggcccgcccg
     2041 caagggtgcg gtcgatcccc tgattcacgc attggaaatg tacccggcga agcagcgcgg
     2101 ctgtttgacc gtcaacagac cggacccgga caacaaggac cgtgcgattt ggctgcatcc
     2161 cggcgagccg gtgtttgaac gcttccgggg gttggtcagc gagcgtctgg gcgaccaggc
     2221 tctgcgcggc gcggtcttca tcgacccgac caccgagaag ccgtacctgt tccacctcgc
     2281 cttgatcagc gtcattcgcc aagctgaccc tgagctgccc gacctggcct ccgaggaaac
     2341 gctcgactgc cagctcgtcg gcgtcaagca gtacgaaggg gccgaggtcg tgctctgccc
     2401 cgttgagcac ctgcttctgc tcaagggcgg ccacggcctg cccgccgccg cacaacgcct
     2461 ggcggtgacg gccgggaagc tcaaagagca ggccgaggcc ttcatcatgg agcgcgtcgc
     2521 ccgggggctt gcggtccagc gccgcaacgc catgttggac acactgcccg agcgcgagca
     2581 attcatccgg cgtgggttcg acttccagga ggccgagctt gccgccgccc gtgtcaagca
     2641 gtcggagaaa gctcgctccg gcaacgcggc cgcgataaag gccctcaagg agatcaagga
     2701 gcagcagcgt tcgctcgccc agcggcgcca cgccgccttg gcgaccatcc ggcgcgaacc
     2761 cgaactgatc gcgcccggac atctgacctt tctggcccac gccctcgtcg tgccctcatc
     2821 cgatccggcc gacctcgaac gtcacgacgc cgaggtcgag aaggtggcga tggacctggc
     2881 ccgagccttc gaggaagcgt gtggggcgac ggtcaaggac gtccacacgc ccgaactggc
     2941 gcgagccgcc gggctgaccg acaaccccgg cttcgacctg ctatcgatct atcccgacgg
     3001 acagcggcgg gccatcgagg tcaagggccg ggccagcacc ggagacgtgg aggtgtcgag
     3061 caacgaatgg gcgcgtgccg ccaacctgcg cgagggctac tggctctacg ccgtctacga
     3121 ctgcgccacg cccaacccgc gactggtgcg ggtacaggac ccgttcggca atctgctggc
     3181 caaggccaag ggcagcgttc tgatcagcgc caaccagatt acacaagccg ccaaggaatc
     3241 tatcgcgtga ccgacttccc aaggttgatt gaacacgcgt ttccgctcaa gcaagcgtcg
     3301 ctggacagcg tgcacgagaa gaacgtccgc cacgggcaca tctcgacgct gcacatctgg
     3361 ccggcgaggc ggccgctggc ggcctgccgg gcggcgctga tcgctacgct cctgcccgat
     3421 ccatcggcaa gtcccaagcc cgagggaatg accgacaccc agtgggaggc cgagatcaag
     3481 cggcgccgcc gggaactctg cgagaagatc ggcggcaagg tggtcacgaa gaacgagaag
     3541 aagaagatgc ccaacggcca gaccgtggag cgcatcaagg aggagaccgt cggcggcatc
     3601 ctgcactggg gccgggagac tgagaacgcc gagacgcttg aatggttccg ccaggaaatc
     3661 cgcaaggcct acggcgggcg cgcccccaag gtgctcgatc cgttcgccgg gggcggggcc
     3721 attccgctgg aggccatgcg gctgggctgc gaggcgacgg ccatcgacat caaccccgtc
     3781 gcctggttca tcctcaaatg cacgctggag tatccgcaga agctggccgg gcagacccgc
     3841 ccgctgccgg agttcattct gcacaacgac gccttcatgg ccgagttctt caagaaggcc
     3901 aagggctacg gcgaggcgga ggtcaccgcc gcattgaagc ggctgcacaa gtcgctcaag
     3961 aagaagacca aggacacggg caagggtagg caaggcgcgc tgccgttcgc gccgttggcg
     4021 agtacggagg gcgaggaact cctggccgac ctggcctggc acgtccgcgc ctggggccag
     4081 tgggtgctgg atcgcgcccg gcgggagctg gcccagtgct accccaccta tgccgacttc
     4141 gagccgatca agaaggacca catcgcctac gagcaccagc cgatgcgcct cgtgccgctc
     4201 aaggacgatg gcacgccgga catcgactcg ctgaacgccg agttcaccga ggagtacctg
     4261 gctgacaagc gcaatccgcg ctgggtcgcc aagccgacgg tcgcctatct ctgggcgcgg
     4321 acggttgtct gcaagaactg ccgggccacg gttccgctgc tcaagacccg ctggctgtgc
     4381 aagaagccca acaagcgggt acttctgacg atggagccga acgccgaccg cacggccgtc
     4441 gtgttcggcg tggatgcgag cgttccagcc aagggtggca acgcagccca gaagcgcgag
     4501 catgacaagc gactcggtgc gggcacgatg agccgggcag gagcgcagtg cccgtgttgt
     4561 ggcgcgatca acacgatgga agacattcgg ttggaaggca aggccggtcg gctgggcagc
     4621 gtcatgaccg ccgtggtcgt cgatacgccc gctggcaagg aatatcgact gcccaccgag
     4681 catgaactgg ccgtcgccac gccgagtgag gagcaggtcc aagccgcgtt caaggatgtg
     4741 cccttcggtg tgcccgatga gccgacgccc gccggcggcg gaagcggtgc gggccgcgcg
     4801 ttcagcgtgc agggctacgg gctgatgaag tggcgagacc tgttcacgcc gcgccaactc
     4861 atggcgttgg gcaccttcgt cgccgccacc cgcgccgccc gctgcgacat ggcattcccg
     4921 caactcccgc ccgagtggat cgaagcgatt tcggcctacc ttgcgtcaat tctcgacttt
     4981 gtcatcaatc gaaacagcac tatctgcaca tggacgctta cacgccaaac cacccgtagt
     5041 acattcgctc gatttgctct cccaatatcg tgggatttct cggaagcgtc agttcttgag
     5101 cagggctccg gcgggtattc aggagcaata gattggatag gacgctgcat cgaccacacc
     5161 ttatccttcg ccacggattg tcctgcgccg tccgtcgaac aacgttccgc aaccgcgccg
     5221 tccgacaagg gatacgatgt cgtactcacc gatccgcctt actacgatgc gatcccgtat
     5281 tccgacttga tggactactt tcatatctgg ctccgtcgaa ctctgttcgg tatatcgccg
     5341 tccgtcgacg ccgcattcgc ccacacctta tcgcctaaat gggatagcga ggccaacgac
     5401 ggcgagctta ttgacgactg tagccgccac gggaatgatg ctgcactaag taaacatgtg
     5461 tacgaagacg gcatggcccg ggtatttggc tcgtgctgtg aggcactgaa accagaaggc
     5521 cgattggtca tcgtctttgc caacaagcag ccagatgcgt gggaaacact cgtatcggca
     5581 atcatacgcg caggctttgt cgttgatgga agctggccaa ttcaaacaga aatgggcaac
     5641 cgcacgcgcg ctcttgtcgg ggccgcgctc gcgtcttctg tctggctcgt ttgcaagaag
     5701 cgtcccgcca ccgccaagcc cggctgggac aacaaggtct tggaggagat gcgggcgaac
     5761 attgcagaga agctgcggga cttctgggac gctggcatcc gcgggccgga ctttgtctgg
     5821 gcggcgaccg gcccggcgct ggaggcgtac agcaagcacc cggtcgtgcg caaggccaac
     5881 gagcccaacg ccacgatgaa cgtgggcgag ttcctcaacc atgtccgccg gatggtcgtg
     5941 gactacgtcg tcggccaggt gctctcggga gaggacccgg agacacgggg acggggagac
     6001 gcggcgattg gcaacttgga cgaggtgacg gcctactacc tgctgcaccg ccacaacttc
     6061 ggcatggacg aagcgcccgt cggcgcgtgc atcctctacg ccacggcgtg cagcctgtcc
     6121 gatgccgaac tggagaagac gtgggacatc ctctcacgca ccggcagcga cgcggctgcg
     6181 gaggaggata gcgaagccga agaatcggcg gatgccgcgg gcgacgccga gcccgccgag
     6241 gattcgggtg gcggcagcaa ggtgaagctc aaggcgtgga accagcgcaa gggcaagagc
     6301 atgggctacg aggccccgga gggcaagccc gtcccgctga tcgaccgcat tcaccgcgtg
     6361 atgcacctgt ggaaggaagg cgacgtccag aaggtggacg agtacctcga ccagcacgcc
     6421 ctgcggcgga acgagctgtt caaacgcgtg gtgcagtcgc tgatcgaact gtccagcaac
     6481 agcgagcggt cgctgctgga gagcatcagc aaccatatcg gcgcgaaggg cgcggtcagg
     6541 gacaaagggc cgacgctttt cgcggatggg ccagtagatg aggagtcgta aatgagtacg
     6601 gacgggtacg acatcacgcc ggacaagcgg ccgatcatcg gtctggtaca gcaggcctac
     6661 gaaggcaagg cctgcctccc gaacttccag cgagacttcg tctggcctcg cgagggggtg
     6721 gcggatcttc tgcggtccgt cctgcggggc tattacatcg gggctctcct gctgctgcgg
     6781 tcggatccgc aggagccgcc ttttgctcca gtgttcctcc gcggcgccaa gccggcgcac
     6841 cgtgaccccc gaccggaact gctgattctc gatggccagc agcggctgac agcattgctg
     6901 tatgccctta cggcgccaca attacccctc aaggactcat cccggccacg gcgtttcttc
     6961 accgatctcc agctcctgct ggaagagccg gacaacgacg gcatcgtgtt cgatgttgcc
     7021 gagaacgagc tcaacggcct ggagcacgaa gaagagcaat accgccgccg catattgccc
     7081 tgcaccacga tgttccgtct cgacgatttc atgacttggc gggacggtct ggacgactgg
     7141 cttcgcgagc aagagccgga gaaccacaaa cgcttccgcg aggagtggcg aacgccgtgg
     7201 acgaaggcgg tcggggcctt ccagaacttc ctggtcccgc tggtcgaact gccgcaggtt
     7261 cgcgattccg acacctttgc gatcggccgc gtgtgcgcca tcttcgagaa gctcaattcc
     7321 acgggggttg acctgtcggt gtatgacttg ttgaccgccc gcctctatcg gcaccgcatc
     7381 cggctgcatc agttgtggaa ggagacctgc cagcaacaca agcggatcgc cgcatggtcg
     7441 ggtggaaagg cggacacgaa caagctcggc gtcctggtgc ttcgcaccct tgcactgctt
     7501 cgcgggctcg agccgaagcc ccgtatcctg atcaatctga aacccgaggg gttcgaggat
     7561 gactggcgaa gagccgccgc ggcgatggag cgggccttcg agctcgtgga acacgttggc
     7621 cctgacggtt ttggcgtctt ccaccagaaa tggttgccgg gtttcggcct gattcccgtg
     7681 ctggcggcgc tccgggcgga gatcgaggag cgcaatctgg gcgaggcggc gcgcgccgag
     7741 ctgcgtcgct ggtactggtg caacgtcttc ctggagcgat tctccagcgg cgtcgagagc
     7801 aagtctcgaa aggactacgc cgagttcctt gaccattggc tgaacgacaa accggagccg
     7861 gccgttttcg ccgaggcccg ggcacgaatc ggggcagccg gatactccat acgggggtcc
     7921 gccagttatg ccagcgcggt gtactgcggc gttttctgcc tgctggccat tcggggagct
     7981 cgcgattggt ctttgggcga gagcatccag ctccagaagc tgaacgacca tcacgtgttc
     8041 ccgcgggcgt acctgcgtga gcacaagatc acgaagaagc cggtggtaaa ctcgatcgtc
     8101 aatcgaacgc tgatcagcaa catgaccaac aacaagatca aggcgaagcg tccttccgag
     8161 tacctggcca tgccggagat cgtgccgccg cttcggggcg atgcggtcct ggagccccat
     8221 ttcctcgacg aagcgtcgcg ggctgcgctt cgtgctactg taccgtcgct ctcgagcgaa
     8281 gaggtcgccg cgcaatacga ggcgttctgc accgcccgcg aacgcacgat cattgccgag
     8341 atccggagag tctgtggtat cgatatggat gtagaagccc caggcggaag gtgagcaacg
     8401 cctggatgag tgaggactga acatggctac aacgaaactg gcgtggatgc cctggcaccg
     8461 ggtggtgaag cttcgggaag acctgaaatc gggcgatcta ccgctgcaca tgttcgcggc
     8521 ggacctctac gaggtcatga tgggcagcgg caagcggccg atctacgaga agcccgagga
     8581 gttcttcgcg ctgaccttcc ccacgcacaa cctgcgcggt ctggtgcggg acgtcgtgct
     8641 gcgcctggcc gggaagaacg acaaggcggt gcggcagctc gagttgacct acggcggcgg
     8701 caagacgcac acgctgatca ccatgcgcca cctggtcaac gatcccgaca acttgcccga
     8761 cctgcccgcc gtgaaggagt tcaccgagac catcggccag acgccgccga aggcccgtgt
     8821 ggcggcgctg tgcttcgaca agctggatgt cgagaccggc tgcgacgtgc ggtcgcccga
     8881 tggcgcggtg cgtcggctca agcagccgtg gagcctgctg gcgtatcaga tcgccgggga
     8941 tgaggggctg aagctgctgc acgccgacgg caaggccgag gagcggacca cgccgcctgc
     9001 cgagaacacg ctgtcgcagt tgctgtcgct gccgctgaag gaaggcatgg ggacgctgat
     9061 cctgctggac gaggttttgc tctacgcgaa ggtgcgctgc cacaccgaac cgggctttct
     9121 ggacgtgttg atcgggttct tccagtatct cagccaggcg gcggcgaagg tggagaagtg
     9181 ctgcatcgtg gcgtcgctgc tgtcgagtga gccgaaggac caggcggacg cgctgggccg
     9241 gaagatcgtc tctgacatgt acgacatctt ccagagacaa cgcgaggaag cggtgcagcc
     9301 ggtggagaag gacgacgtgg ccgaggtgct gcggcgtcgg ctgttcgatc cccagtcggt
     9361 ggagcttcga gggcagtggc cccagcacgt gatcgctgcg ctcaagggca tctcggccct
     9421 cgacgaccag acagccaagc aaggcggtgc ggccgaggag cggtatctca agagcttccc
     9481 gttccatccg gaactgacgg aggtgttcta caccaagtgg tcggcgggca tcgagcggtt
     9541 ccagaagacg cgcggcgtgc tgcggacgtt cgccctggcg ttgcgcgagg cagagaagtg
     9601 ggatgaaagc ccgttgatcg gcccggcggt gttcctcaac gctgccaaac aggaagggct
     9661 gtccgattcg gcgcgcgagc tggtcgcggt tgcggacacc atcgtcaccg acggccaggc
     9721 gacgaaatgg acgggcatcc tcgagacgga gctttcgtgt gcccgccagg tgcagtcgga
     9781 atccgtcggg ctgaagctcc gcgagatcga acaggcggtg atggcgacgt tcctgcactc
     9841 gcagccgacg ggacggagcg cgaaggcacg cgacttgatg ctgctgatcg ggccgtgccg
     9901 cccggacaag atcgagctgg agaagggcct ggcacgatgg gcacggagca gctactggct
     9961 cgatgatacg aacctccctg aaaaagacgg gcaactgccc aacgagtggc ggcttggcaa
    10021 ccgaccaaat ctgaaccaga tgcacgccgc cgcggcggcg caggtctccg acgacgcggt
    10081 gaaagcccgt ctggtggagg aaatcggcag gaccaagtca ctgaccagcg gggcgtcggc
    10141 gttgggcgtc cgtgttcaca ccttgccgac caagcccaag gacatcgagg acgatggcct
    10201 gttccactac gcgatcctgc cgcccagcgc tgcctcggat tcagggaaac cgagcgctga
    10261 ggcaaagcga ttcctcgatg aaacgaccgg tgcggagaag ccgcgcgtgt tccgcaacgc
    10321 cgtcctgctg ctcgcaccat cccgtgacgg cctcaacgtg gctgccgcac gggtgcgcga
    10381 tcatctggcg tgggaaaagg tcatggacga cctggtgccc aagaacgagg acgaaaagaa
    10441 gcagaagggt ccggtcgaca tcgcccgctt gcagacgctc aagatcaaca tcgacaaggc
    10501 caaggcgcgc gttccggagg cgatccgcca agcctactgc atcgtggtga ccgtttcgga
    10561 caaggacgac gtgcaggcat tcaagatcac agtcaccgac gaagcgcact tcgagacgat
    10621 caagaacgac ccacggtcgc gggtgcagga caaggcggtt acggccgagg cgcttctgcc
    10681 caacggcccg tacaacctgt ggaagggcgg cgaaaccagc cggcgcgtca aagacctcgc
    10741 cggcgcattc gcgcagctcc cgcacctgcc gaagatgctc aagtcgcagg cgatcatgga
    10801 gacgctagtc gacggctgcg tgcagggcac gtttgtgctg aagctcacgc gcccggaccg
    10861 tacgttccgt acttggtggc gcacgcggcc cgacgatacg gcgctggccg atccggcgct
    10921 tgagctggtc cttcccgaag ccgccgagct tggcagtatt cccggtgagt tgctcgcgcc
    10981 gaaggcgctg cccgaccttt ggaccggtga cgaggtcacg gtccaggcag taacgcagta
    11041 cttcagcggc aacacggtgg ttcaggttga caagggcggg ttcaaggaac ccgtaccggt
    11101 tcccaaggca gcgacggacg tcgtcaacgc cgccgtcgca gaggcggtcg ccaatgggaa
    11161 ggtgtggttg ttgtcggggc cggccagtct gctggccgaa ccgattccga ccggcgtgct
    11221 ctccccggca tccaggttgc gggtgcctcc gttacccatt gcggcggcag cgatccttcc
    11281 agagaacctg ccgacggcat ggcaggacgg cgcgacgacg gcgctggcca tcgccaccgg
    11341 cctctcccag caggcaggct acacgctgcc gtggaagacg gtgcgggacg tcatcggcgg
    11401 atcgctccag gctcgcttca tcgccctggc cgaagactcc gcgccctggc cctgcgatct
    11461 ggccgctgcg cagacggtga agatcaaagt tgccgctgcc gggaccggtg gtggaaccgg
    11521 aggcggcacc ggtggcggga ccggcggagg cggtgcaccg cttccgaatg tccgtgtcgc
    11581 ggcggccgac tttgagccgt cgcagatgca ggacctcggg gatctcgtcc cggcgctgct
    11641 ggacatcaag gccaaggcga aggtcgcgat gaagttccac gtccgattgg aggtcggtga
    11701 tgcgggctcc cggccgtcgg acgatgtggt tggcgatatc aacaatctgc ttaaggactt
    11761 aggagaaggc ttccggctcg tttgagtatt ggtcggatga tgcttgacta cctaacaaaa
    11821 accgaatatg ctgagtgtcg atccgctcgg ggaagcggat gccgtccatt tcagccgggc
    11881 ctggccacgg aatcgggcac caggagggtg cggcccgggg gcttcattca tacggaggca
    11941 ctatgggccg ccagtactcc gcgaaaacgt ttctgcgcaa cgctcctaac cgcctgctgc
    12001 agcagtactt ccagaagcag gacctcaacc tggggctgga ctgggtccgc ctggacgaga
    12061 ccgaagtcaa cccgatcttc ctcgccctgg aggccttgcc cgcagccacc cgtagcgatg
    12121 tggacaccga tttcgccctg atccacgaga tggcatgtga gcacggcgtc caagcgatcg
    12181 tggaggaagg ccggaactgg ggtcgcgatt gggccgagca gttcgccggc atgcgcaacc
    12241 attacgaacg ggcgttctgg accttcctga acgaaccccg tcggtttcac gtggccggct
    12301 gcttccacga gatggaccgc ctcggcggct ggcgccggcg gttcgtgggc cggaggctgg
    12361 aggcgcagga cgacgaggac gccttggccg aactcgaaag acgcgtgcgg ctcttctatc
    12421 gacgtcaggg gcgcgggcgc ttctgtcacg tcgattacta cgaacgccgt gagcccgagc
    12481 ggcattgcta cttcgcctac cccgaggact acgccagcac cgacgtggga tacgacgaga
    12541 atggccgttt cctccatcgc ccacggcgcc cggcgttcga ggtgatcttc gtctatcgcc
    12601 cggaggaggg cgtgctcgag ctgcatgcca agggcaacaa gcggcaggct gacgaattgg
    12661 cggagatctt ctgcaccacg actctcgggc tggtgaaact acccgacgac aacgatcgcg
    12721 tgcccttcga cctggccgtg ctgaagaacc cggagttccc gttcccgacc gatcctgctg
    12781 accgcatctc ggccgtacat gtgcggcaat tgcggtttga cctgcccgag ccaacgcgtg
    12841 gccggatcac ggtctccgcg cacgcgtcgg accatcgtcc gcgggcgctg cgcgatgtgg
    12901 tggaccgggc gatcgaccga gcccggatgc cactcgaggc cctgcacgtc tcgcaggcga
    12961 agatgcggct ggtctttgca ccccacaacg gcgagcgtcc caagacattg accttcgagg
    13021 tcacgtaccc ggatcgctgc acgctcaagg atgaccctct ggatcagatc gccaagaagt
    13081 acctgagcca atgggggatc gctcgtgact gatgcaatac gccacatcat gcatggcttt
    13141 gaacggggcc ccgtagttgc cttcaccggc caggaaaccg cgcgttggcc gcgtggtttg
    13201 ctcgccgcac tcgtcgatgg tgccatcctg cacgaaaccc agctggccga ctcagtctgg
    13261 tgcgatgaat gtgaggaggg ttgttcgatt acgcccgact tgcacgagca tcctggga
//
LOCUS       DAOTPW010000158        13253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4534_length_13253_cov_5.487650, whole genome shotgun sequence.
ACCESSION   DAOTPW010000158 DAOTPW010000000
VERSION     DAOTPW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13253
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4534_length_13253_cov_5.487650"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="P5316_14270"
     CDS             <1..982
                     /locus_tag="P5316_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86169.1"
                     /translation="CALLWWRRSRKTGIALVLGLLAATSLSISLQSLLFLAIAVPAML
                     LSTRGAHGGRITGPITTLTAFVVGTTILTTAIALPRLPTVLSNGPLKKEAALSPSQTR
                     HAWPHAAAPLVAHAAHPGDALRLRAALKFFLIRDRLRDDLRQIVTRFHTDLWNAIEDG
                     SGSHIAQAALAASPPGTADHPPAGRFLIDQCRDAPAQTLTWLAQRMWKSIYTTRDHRT
                     HYPFILLQFLWLIPAFWGLWIALRYPPWRWFAAAAGLFTGCHWLLVALAEPLARNLVP
                     VGGFAILLALVGVTDVYERFFGRRLTAPAPASRPARLKRIQQNLSNSPHD"
     gene            1215..1787
                     /locus_tag="P5316_14275"
     CDS             1215..1787
                     /locus_tag="P5316_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRR86170.1"
                     /translation="MPIRVTTAKRAFSLLYRNLAEVVHVEEDQYLSYDFESWREVSEL
                     KQRFEPERHDWVRTVRFSLVVPRIIRLLFYDRLPRRGVKFNRRNIFARDNNCCQYCGR
                     KYPTSELSLDHVIPRSQGGTTTWENVVCACLKCNIQKGGRTPEQAHLKLIAKPTKPRR
                     SPVLNLKLSHDKYRSWKQFLDHAYWNVELK"
     gene            complement(1858..2658)
                     /locus_tag="P5316_14280"
     CDS             complement(1858..2658)
                     /locus_tag="P5316_14280"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRR86171.1"
                     /translation="MIERSHGDVTLLSFESLEAVPRLVHAVTTRPQNYAPHRGSGREE
                     AVHWRRRVCEVLGMPFESLTSPEQVHGADVLSLEPGDVGRGRDGRHGAIAFVDGLMTD
                     MPNVPLVILSADCPLILVYDPDRPAVGVVHASWRGTVAGAADNLVKQMVRSYGSDPAR
                     MRAAISPSAGPCCYEVGPHVRRIAKTRLADADNCFVEQGGRVLFDLWTANRDQLAAAG
                     VPSQAIEIAGLCSICDRRFWSHRRDGAEAGRTALFVGLRGPLAFNDRA"
     gene            complement(2687..3643)
                     /locus_tag="P5316_14285"
     CDS             complement(2687..3643)
                     /locus_tag="P5316_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRR86172.1"
                     /translation="MNALVFNQMLRFDAKYPEPLPGEDEALIEVKLAGVCATDIEITK
                     GYMSFAGVLGHEFVGTVVKGSRRWRGRRVTAEINCVCGKCDMCQHGLANHCRKRSVIG
                     ISRRDGCFAQYIAVPERNLHEVPEALSDEQAVFIEPLAAAYQVIKQCPIEKRTRVAVV
                     GSGRLGLLVAQVLRTTGCRLEVIGRNGHTLDFCEKKGIQTIPLSDLVPKTDRDVVVDC
                     SGSPEGFETAVKLVRPRGTLVLKSTYAGGREINLAPVVVNEIAVLGSRCGPFPDAIAA
                     LARRDIDVESMVSRQLPLARGVEAIELAADPRYIKILLKVKG"
     gene            3872..4681
                     /gene="trpA"
                     /locus_tag="P5316_14290"
     CDS             3872..4681
                     /gene="trpA"
                     /locus_tag="P5316_14290"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRR86173.1"
                     /translation="MTNRIERAFEDLSAKGKKGLLPFITAGLPDLDTTQRILESLTSL
                     GVTAVEIGFPYSDPIADGPTIQESFTRVLQRGIRVRDILAMVARFRQKSQLPLLAMLS
                     YSIVHRIGLDTFVCQAAQAGIDGLILPDLSLEEAPQTAKVVADAGLCLPMLVSPVSPR
                     WRREKIAATCTGFVYYMSVTGITGERKELPPDLVDNVREIRRASGRPVVVGFGISSAE
                     HVRLVCSAADGAIIGSALVRKIMQAANGGANKDAVVAAAADAVREWMTGLP"
     gene            complement(6020..7021)
                     /locus_tag="P5316_14295"
     CDS             complement(6020..7021)
                     /locus_tag="P5316_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86174.1"
                     /translation="MKTRLGMIGMGGIGRFHADYLVTHAEAELTAVCDIDEARLAGAA
                     AKYGSRPFTDYRDMISKVDLDAVYVCVPPYVDGPPEIDCLDAGLHIFVEKPIALDMQT
                     ARKVEKKIADSGLIAAVGYHWRYMGAVDLANEMMGDEPISFLQGRWVGGMPEVFWWRK
                     MGMSGGQFTEQCTHIVDLARYFAGDVTLVSAGGVKGQMCRRVPDHDVWDAQAAVLHFA
                     NGLIASIHTAHLGEWFSDYGLRVQTPDSCFEINEIPWSSKLTVRRKGEVCEFLGPQRG
                     WKEAHYVESDVFIRAVRTGDRSEIRSDYSDAVKTLAVNLAITRACETRQTVAVGEVR"
     gene            complement(7042..7461)
                     /locus_tag="P5316_14300"
     CDS             complement(7042..7461)
                     /locus_tag="P5316_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRR86175.1"
                     /translation="MARRTGPPVMVGAIIDRVSHGVIETLIARIPTPPYEGCWTFPSG
                     PADPGEAPEAALRRMLHATLGVSLRIICGQPPIDLPWDDVLCRWRFFFCDATGSELTS
                     RYYAEVRWVRRPDFREYDFDPVSQQVAAWLLDDLPRE"
     gene            7582..9774
                     /locus_tag="P5316_14305"
     CDS             7582..9774
                     /locus_tag="P5316_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR86176.1"
                     /translation="MRITTTTLERMAQQTKPPSTFGPFKRLLGYVWRHKRFILPSIGC
                     ILVMAVTYTASIGSVLPVLSVLIRPQGLHGSMNQYIAEKRLRCVFVLYSSLKDGEIPG
                     VPEGCARLFSARAASPLAHNNGGLPTGALILAVNDTRGPAVDVFRALAESGPQLAITW
                     RTEVMATETTTTVTAPPVPFYHQLAQRVLQIVPGGLRPHEKWRTLLVVLGMLLVVVLI
                     GNTARYFAEYFTVVANCRAIIDLRRELYAKVLQLPLSHFSRNTADTMSRFVQDTNEIF
                     RGLANFFEKIVTEPFKAVGAAAFALWLDWRLTVAVIVAAPLVVLLIRKLGNKIRKANR
                     RLLMAYSAMMSTLQSTLEGMRVVKGYCQENYERRRLFAVDHQVLLQQVRMGRIEALAS
                     PTLETMGFVAVMLAILYFGNGVLAQGAEEKIPDFLAMVVCLGAMFDPIRKLSTVYPKI
                     QRANAAADRVFELLDSPTEDDEDEGRPKLRPIRESIEIRNITFTYPEATQPALQDVSL
                     TVRKGEIIALVGPNGSGKTTLVSLLPRFFPIQQGQILIDGQDITRVSLRSLREQFSLI
                     TQQSVLFPDTIRANIAYGRLDATDAEIEEAARKAHADEFIRQLPQGYDTVIGERGATL
                     SGGQQQRIAIARAILKNAPILIFDEATSQIDPESELKIHQAMDVFFRDRTAFVIAHRY
                     STISEADRIVVMDAGFILAVGTHEELLQTCPLYKRLCETQLRGSLLDE"
     gene            9859..11529
                     /locus_tag="P5316_14310"
     CDS             9859..11529
                     /locus_tag="P5316_14310"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRR86177.1"
                     /translation="MQTIARVLTQHGFGHLVDSLHLERYVPLPKRWQQAVMPSLEGEP
                     EASIGRRIARVCEELGPTFVKLGQLLSSRPDLVSADIIAELVKLQDRVPPFPTDQARR
                     IIETDLGMPLSQCFAGFGDEPFACGSIAQVYRATTVAADERPSCRVVVKVKRPGIEDT
                     IRLDMTILRWIGDVAERLVPELAVYHPKMIVDEFEQTMMRELDFVNEAATIARFAESI
                     GEDPDFRVPTVFWEFTGPSVLTLEEMHGVSIQTLLTRPDPAIDRKQLADRLARGFVRQ
                     FFETGLFHADPHPGNLLVEPPAAIRLIDFGLTGRIDEEMLGHLVMALMGAVNREPEIV
                     VEVLADMDALGEQTDRVQLRRDFLELIEKYYGLPLHRFDMQTLFHEITALIRRNDVTV
                     PREFVLFGKALVAVGGICLQLDPEMDLVTLVKPRLVKLLAERMAPGKLLKSAAMSGWH
                     LLNFLKGFPSQLRDVSRRMARGKWQINIRHQNLDFLANEIDRASNRLSFAVITAAIII
                     GSSWVLASSSATAVLGVPTWSLGVVGYVLAGLMGLWLVIAIVRSGKLS"
     gene            12233..12304
                     /locus_tag="P5316_14315"
     tRNA            12233..12304
                     /locus_tag="P5316_14315"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12265..12267,aa:Thr,seq:cgt)
     gene            12565..>13253
                     /locus_tag="P5316_14320"
     CDS             12565..>13253
                     /locus_tag="P5316_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86178.1"
                     /translation="MNRSARIGRRTLLKASGMLTGGILGLPYLVSHRALAAAGRPGAN
                     DRLTVGHIGVGGMGTYHLRNMVERMKRGQVNVVAVCDVDESRLAEASRNAGPQADVYR
                     DYRYILERKDIDVVVIATPDHWHAVQTVHAAECGKHVYVEKPACCTIEEGKAMVRAAR
                     ENRISVQVGSQGRSEHEAYLAHRYLVNDNIGRIDRVWCFHYPSPSDDNPVPDSEPPPE
                     LDWDLWLGPMR"
BASE COUNT         2469 a         4597 c         3650 g         2537 t
ORIGIN      
        1 gtgcgctctt ttgtggtggc ggcgatcgcg aaagaccggc atcgccctag tcctcggttt
       61 gttggccgcg acgagcctgt cgatcagcct tcaatctctc ctgtttctcg cgatcgcggt
      121 gccggctatg ctcctctcca cgcgcggagc acacggcggc agaatcaccg ggccgatcac
      181 caccctgacg gcgttcgtcg tgggaacgac aatccttacg acggccatcg cactgccacg
      241 attgcctacg gtactgtcca acggcccgct caaaaaagaa gccgccctgt caccttctca
      301 aactcgtcac gcttggcccc acgctgccgc cccgcttgtc gcccacgcag cccatccggg
      361 cgacgctctc cgcctccggg cggctcttaa attcttcctg atccgagacc ggcttcgtga
      421 tgacttaagg cagatcgtca ctcggttcca taccgatttg tggaacgcga ttgaggacgg
      481 cagcggctca catatcgccc aggccgcctt ggccgcctcg ccgcctggca ccgccgatca
      541 cccccccgcc ggaagattcc tgatcgacca atgccgcgac gctcccgctc aaacgctcac
      601 ttggctggcc caacgcatgt ggaagtcgat ctacaccaca agagaccacc ggactcacta
      661 tccgttcatc cttcttcagt tcctctggtt gatcccggcc ttctggggct tgtggatcgc
      721 ccttcgctat ccaccctggc gatggttcgc cgccgctgcc ggcctcttca ctggttgtca
      781 ctggttgctg gtagctctgg ccgagcctct ggcccggaac ctggtgccgg tgggcggttt
      841 cgccattctc ttggcactcg tgggagtcac cgatgtctac gagcggttct ttggccgtag
      901 actgaccgcg cccgcccccg cttcccgacc agcccgactg aaacgcattc agcaaaacct
      961 cagcaacagt ccccacgact gagcattcgg ccggggtgac aagtcccgca ctctgccgtt
     1021 gttgcagcct cggacaagtc accttataat gcgcacaagt agtagatgca gggctggcga
     1081 cggccgggtc cctgccacat gggactcgcg ccgcaacgga gggtgttgcc ttcccgacgg
     1141 cgatcgcgac gagtactgtt ttcgacccgc tttcaggcct gagtcatcat gtcctcatcc
     1201 tgaaccgcca ttatatgccc attcgggtca ctaccgccaa gcgggccttc tccctgctct
     1261 atcgcaactt ggccgaagtc gttcacgtcg aggaggacca gtatctctcc tacgactttg
     1321 aaagctggcg ggaggtcagc gagctcaagc agcgctttga acccgaacgc cacgactggg
     1381 tgcggaccgt ccgtttcagt ctcgtcgtgc cgcgcatcat tcggctcctg ttctacgacc
     1441 gccttcccag gcgcggagtg aagttcaacc gccgcaacat cttcgctcgc gacaacaact
     1501 gctgccagta ctgcggccga aagtacccga cctccgagct gagtcttgat catgtcatcc
     1561 cgcgcagcca gggcggaacc accacctggg aaaacgtcgt ctgcgcctgt ctcaagtgca
     1621 atattcagaa aggcggccgg acccccgagc aggcccacct caagctgatc gccaagccga
     1681 ccaagccccg acgtagcccg gttctcaacc tcaagttgtc acatgacaag tatcgctcct
     1741 ggaagcagtt cctcgaccac gcatactgga acgtcgaact gaagtaagcc cctcaaggca
     1801 aaactcacgt ctgtctctat cacccgacct ctgatcgccc cgccgtggcc ccggccgcta
     1861 cgcacgatca ttgaatgcga gcgggccccg caagcccacg aagagcgccg ttcgcccggc
     1921 ctcggcaccg tcccgccggt gactccagaa ccgtcgatcg cagatactgc acaggcccgc
     1981 aatctcaatg gcctggctcg gaacgcccgc cgcggccaac tggtctcggt tggcggtcca
     2041 gaggtcaaac agaacgcgcc ccccctgctc gacaaagcaa ttgtccgcgt cggccagcct
     2101 cgttttggca attcgtcgca catgcggacc aacctcgtag cagcatggac ccgcagaggg
     2161 tgaaatggcc gccctcatcc gggcagggtc gctgccgtag ctccgcacca tctgcttcac
     2221 gaggttgtct gcggccccgg ctaccgttcc tcgccaactg gcatggacca cccccaccgc
     2281 cggtcgatcg gggtcgtaaa cgagaatcag cgggcagtcg gccgagagga tgaccagcgg
     2341 gacattcggc atgtcggtca tcaggccgtc aacgaatgca atcgccccat gccggccatc
     2401 gcgaccgcga ccgacgtcgc ccggctccag actcagaaca tccgctccgt gaacctgttc
     2461 cggcgaggtc agcgactcaa aaggcatgcc gagaacttcg cacacccgtc ttcgccaatg
     2521 aacggcctcc tcgcgaccgc tgccccggtg aggggcatag ttctgcgggc gggtcgtcac
     2581 ggcatgaacc agccgcggca ccgcttcaag gctctcgaac gagagcaacg tcacatcacc
     2641 gtgcgatcgc tcgatcatct tgaagaccgt accccacaga aaaagcttat cccttcacct
     2701 tcaagaggat cttgatgtag cggggatcgg ccgccagctc gatcgcctcg acgccgcgag
     2761 ccaacggtaa ttgacgcgac accatcgatt caacatcaat gtcgcgccgg gccagcgctg
     2821 cgatcgcatc aggaaacggt ccgcaacgac tgccgagtac cgcgatctcg ttgacaacca
     2881 ccggcgcaag gttgatttct ctcccgccgg cataggtgct cttcagaacc agggtgcctc
     2941 gggggcgaac gagtttcacg gctgtttcga agccctcggg gctgccgctg cagtccacga
     3001 ccacgtcccg atcggttttc ggcaccagat ccgacagcgg gatcgtctga atccccttct
     3061 tctcacaaaa gtccagcgtg tgcccgttcc gcccaataac ctccaggcga caaccggtcg
     3121 ttcgcagcac ctgggcaacc agcaaaccca gtcgtccgga ccccacaacc gccacccgcg
     3181 tccgcttctc gattgggcac tgcttgatca cctggtaagc cgcggccagc ggctcgatga
     3241 agaccgcctg ctcgtcactg agggcttccg gaacttcatg cagatttcgt tccggcaccg
     3301 ctatatattg ggcaaagcac ccgtcccggc gtgagatgcc gatgacgctt cgcttgcgac
     3361 agtggttggc cagaccgtgc tggcacatgt cgcacttgcc gcagacgcaa ttgatctcag
     3421 ccgttaccct ccgcccccgc caccgcctcg atcccttcac caccgttccg acaaactcgt
     3481 ggccaagtac cccggcgaaa ctcatatacc ctttggtaat ctcgatatcc gtggcgcaga
     3541 cacccgcgag cttcacctca atcagcgcct catcctctcc tggcaatggc tccgggtatt
     3601 tcgcgtcgaa ccgaagcatc tggttgaaca cgagcgcatt caccttcgta gccttctcta
     3661 tcagcggtca aatctcctca gcgcggacca gtatcgagca tcacgccggg accgtcaatc
     3721 cgcaacccct taaacattgg cccgcgctcc ggttcacgtc tgctggcctg ccgcctcgtt
     3781 cgccgatata gtcacgattt cggaaacggt ggcctgcggc cgtggccggc ttgccctgcc
     3841 gcacggcgtt gacgagatcg aaggtgtgat catgaccaat aggattgaac gggcgtttga
     3901 agacctctcg gccaagggta agaagggcct cctgcctttt atcaccgccg ggctgcccga
     3961 ccttgatacc actcagcgca ttctcgaatc cctgaccagc ctcggtgtga cggccgtcga
     4021 gatcggcttc ccctactccg acccgatcgc cgacgggccg accatccagg agtccttcac
     4081 cagggttctt caacgcggaa tccgtgtccg agacatcctc gcgatggtgg cccgatttcg
     4141 gcagaaatct cagttgcccc tgctggcaat gctgtcctac tccatcgtgc accgcatcgg
     4201 tctcgacacg tttgtctgcc aagccgccca ggcgggtatc gacgggctga tcctgcccga
     4261 tctgtcactc gaggaggccc ctcagaccgc caaggtcgtc gccgatgccg gcctctgcct
     4321 gccgatgctt gtttcgcccg tctcacctcg ctggcgacgt gaaaaaatcg ccgcaacctg
     4381 caccggcttc gtctactaca tgtccgtgac cggaatcacc ggcgagcgaa aggaactccc
     4441 gccggacctc gtcgataacg tccgagagat tcgccgggcc tcaggccgcc ccgttgtcgt
     4501 cggttttggc atcagttccg ccgagcatgt gcgattggtc tgttcggccg ccgacggcgc
     4561 aatcattggc agcgcgctgg tccgcaagat catgcaagca gcaaacggcg gcgccaacaa
     4621 agacgccgtc gtcgccgccg ctgccgacgc cgtccgcgaa tggatgaccg gcttgccgta
     4681 aacccccgaa ccgatcgccg catgccagac acttattgtc tccgcccgac gctcgatcgc
     4741 cgcgagattc gcgtcggcgt gggacttcag aatgacgcca cagagtgcgt attcaagata
     4801 ctgtatactg tagccaagtc tcaatcgatt cggcctgatt ccacgcggcc cctggctcat
     4861 ctcgtcgccc gccatgaacc ggctgctgcc ttagtctctt ccgtcgcgac tggtgtgcat
     4921 tttgcgcgca tgtttcttga tcgcagaacg cttgggttgc ggcccgcaaa aaggccatcc
     4981 gccttagcca ttagcagatg ggttacgtcg gccagacgcc atccccacgg ccgtgcgtgg
     5041 gcatgcgggg gtagggccgc tcggcccgga atcctttccg ggccacatcc tccgcggaat
     5101 ccttttccgc tcggcccgga atcctttccg ggccgaaccc cctgcggaat cctttccgca
     5161 ttcagcgccc atcacgatgg caatcttcca ccagcaggta gggtaggttc gtgctcggcc
     5221 cggaatcctt tccgggccac atcccctgct cgctcggccc ggaatccttt ccgggccgaa
     5281 ccccctgcgg aatcttttcc gcattcggaa tccatgacga taacaatcct ccaccagcag
     5341 gtagggcagg cccgccgggt gccatgccca cggctttgcg tgggcatgcg cctcaaaatg
     5401 aatcctgaac taggtaaagc gggtcagagc cccgccaagt cgccccgacg aagcagggcg
     5461 tcgacctgcc gccaccctga ccactccccc gcgccacaca aacccaacat caagcgcaac
     5521 cgcaattcgc catcgctccg ctccacccgc ccgttgcgga gcatcccccg tgttctgtcc
     5581 cttgtctagc tgacgactga gagctgattg ctgaaagctc ccctgcccgt tctttgaaat
     5641 ctgaagacca accccctccg gcccaaagcc gggagccaac acctcaacaa ctctctgcga
     5701 ccaaacgaac ccaccgagcc gctcggcccg gaatcctttc cgggccgtac ctcttgcgga
     5761 atcctttccg cattcagcgc ccatcacgat ggcaatcctc caccggcagg tagggcaggt
     5821 cagagccctg agaagtcaga ccgacgaagc acggcctcga cctgccgcca ccttggccgc
     5881 tccaccgcgc caaacgaacc caaaagccgc gagcctgtct taatataagg tattatgaat
     5941 caatgactta tacgcgacca ggcgcacgcc caccggccgc tcggccaaca tctgataccc
     6001 cccaatcgag cggttcttgc tacctcacct cccctaccgc caccgtttgc ctcgtctcgc
     6061 aggctcgcgt gatcgccagg ttcaccgcta gggtcttcac tgcgtcactg tagtcgctgc
     6121 gaatctccga ccgatcaccc gtgcggacgg ccctgatgaa aacgtcgctc tcgacatagt
     6181 gggcttcttt ccagccacgt tgcgggccga gaaactcgca cacctcgccc ttgcggcgaa
     6241 cggtcagctt gctgctccag ggtatttcgt tgatttcgaa gcaggaatcc ggcgtctgca
     6301 cccgcaagcc gtagtcgctg aaccactccc ccagatgggc agtgtggatc gaagcgatca
     6361 ggccgttcgc aaagtgcagg acggccgcct gagcgtccca gacgtcgtgg tccggcaccc
     6421 gcctgcacat ctggcccttg acgcccccgg ccgaaaccag ggtcacatcg cctgcaaagt
     6481 acctggccag atcgacgata tgcgtgcact gctcggtaaa ctgcccacca ctcattccca
     6541 tttttcgcca ccaaaaaacc tcaggcatcc cccctaccca ccggccctgg agaaagctga
     6601 tcggctcgtc gcccatcatc tcgttggcca gatcgaccgc tcccatgtac cgccaatggt
     6661 agccgaccgc cgcgatcaac ccgctgtccg cgatcttctt ctcgaccttg cgggccgtct
     6721 gcatatcgag agcaatgggc ttctccacaa atatgtgtag ccccgcgtcg agacagtcga
     6781 tctccggcgg cccgtccacg tacggcggca cgcaaacgta caccgcgtcg aggtcaacct
     6841 tcgagatcat gtcacggtag tccgtgaagg gccgcgaccc gtatttcgcc gccgcgccgg
     6901 caagccgggc ctcgtcaatg tcgcagaccg cggtcagctc cgcctccgcg tgagtcacca
     6961 ggtaatccgc gtggaaccgg ccaatgcctc ccatgccgat cataccgagc cgagtcttca
     7021 cgttgtcctc cgacgtacga gtcattcccg cggcaggtcg tcaagcagcc acgcggcaac
     7081 ctgctggctg accggatcaa agtcgtactc gcgaaagtcc gggcgccgca cccagcgaac
     7141 ctccgcatag tagcggctcg tcaactcgct gccggtcgca tcgcagaaga aaaaccgcca
     7201 gcgacacaaa acgtcgtccc acggcaggtc gatgggcggc tgaccgcaga taatccgcaa
     7261 gctcaccccg agcgtggcat gcagcatccg acgcagcgcg gcttccgggg cctcacccgg
     7321 atcggccggc ccgctcggga aggtccaaca accttcgtac ggcggcgtcg gaatcctggc
     7381 tatcagcgtc tcgattaccc catggctcac gcggtcgatg atcgctccga ccatcaccgg
     7441 tggcccggtt cgtctggcca tgaggacgct ccttctcgcc cggaaccgca gggtcgctgc
     7501 caggagtcgc tcggccgccg atcggcacct cccgccgcat gcagtagtcg atcggggact
     7561 atcccggtat cataccgccc catgcgtatc actacaacga cactggagcg tatggctcag
     7621 cagaccaagc cgccctcgac ctttggaccg ttcaagcggc tcctgggcta cgtctggcgg
     7681 cacaagcgct tcatccttcc ctccatcgga tgcattctcg tcatggccgt gacgtacacc
     7741 gccagcatcg gctcggtcct gccggtcctg tcagtcctca ttcgcccaca aggcctgcac
     7801 ggctccatga accagtacat cgccgaaaaa cgcctgcgat gcgtcttcgt gctttacagt
     7861 tcgctcaagg acggcgagat ccccggcgtc cccgaaggct gcgctcgcct cttcagcgcc
     7921 agagccgctt cgcctctggc tcacaacaat ggcggcctgc ctaccggtgc gctcatcctg
     7981 gccgtcaacg acactcgcgg tccggccgtg gacgtctttc gcgccctggc ggaatccggt
     8041 ccccaactcg ccatcacttg gcgaacagag gtaatggcaa ccgaaaccac gaccactgtt
     8101 accgctcctc ccgtgccctt ctatcaccag ttggctcaac gcgttctcca gatcgtgccc
     8161 ggcggcctgc gcccgcacga gaaatggcgc accctcctgg ttgtgctggg aatgttgctg
     8221 gtggtcgtgc tgatcggcaa caccgcacga tacttcgccg agtatttcac cgtcgtggcc
     8281 aactgtcgcg ccatcatcga ccttcgccgc gagttgtatg ccaaggtcct ccaactgccg
     8341 ctcagtcatt tcagccgcaa caccgccgac accatgagcc gcttcgtcca ggataccaac
     8401 gagatcttcc gcggattggc caacttcttc gaaaagatcg tgaccgagcc cttcaaagcc
     8461 gtgggggccg ccgcttttgc tctctggctc gactggcgcc tgaccgtggc cgttatcgtc
     8521 gccgcccctc tcgtcgtgct ccttatccgc aaactcggca acaagatccg caaggcaaac
     8581 cgccggctgc tgatggccta ctcggcaatg atgagcacgc tgcaaagcac acttgagggc
     8641 atgcgcgtgg tcaagggcta ctgccaggaa aactatgagc gacggcggct gtttgccgtc
     8701 gaccatcagg ttctcctcca acaggtgcgc atgggccgta tcgaggcttt agcttcccca
     8761 actttggaaa caatgggctt tgtcgcggtc atgctcgcga tcctctactt cggcaacggc
     8821 gtactcgctc aaggcgccga ggagaagatt cctgacttcc tggccatggt ggtctgtctc
     8881 ggcgccatgt tcgatccgat ccgcaagctc agcaccgtct accccaagat tcagcgggcc
     8941 aacgcggccg ccgaccgggt gtttgagctg ctcgacagcc caaccgaaga cgacgaggat
     9001 gagggacggc ccaagctccg tccgatccgc gaaagcatcg aaattcgaaa catcaccttc
     9061 acctatccgg aggccaccca gcccgccctg caggacgtct cgctgaccgt caggaaaggc
     9121 gagatcatcg cgctcgtcgg ccccaacggc tcaggaaaaa ccacgctggt cagccttctg
     9181 ccccgcttct tcccgatcca gcaggggcaa atccttatcg acggacaaga tatcacccgc
     9241 gtttcgctcc gatcgctgcg tgagcagttc agcctgatca cgcagcagag cgttcttttc
     9301 cccgacacga ttcgcgcgaa cattgcctac gggcgcttgg atgcaaccga cgcggagatc
     9361 gaagaggccg cccgcaaggc tcacgccgac gagttcatac ggcagcttcc acagggctac
     9421 gacaccgtca tcggtgagcg gggcgccacg ctctccggcg gccagcaaca gcgcatcgct
     9481 attgcccgcg ccatcctcaa gaacgccccg atcctcatct tcgacgaggc caccagccag
     9541 atcgaccccg agtccgagct gaagatccac caggcaatgg atgttttttt ccgggatcgc
     9601 accgctttcg tcattgccca ccgttacagc accatcagcg aggccgaccg catcgtggtc
     9661 atggacgccg gctttatcct ggccgtcggc acccatgagg aactccttca gacctgcccc
     9721 ctctataagc ggctctgcga gacccagttg cgcggcagcc tgctggacga gtgagtcttt
     9781 cattgaccac ccggcaagtt cgcgtatgct gttgaagcca tgttccgtct ccccaagtca
     9841 gttcgaacga tccgccgtgt gcagaccatc gcccgcgtat tgacccagca cgggttcggc
     9901 cacttggtcg acagtctcca cctcgaacga tatgtcccct tgcccaagag atggcagcag
     9961 gcggtcatgc catcgctgga aggcgaacct gaagccagca tcggccgacg tatcgcccgc
    10021 gtttgcgaag agctcggtcc caccttcgta aagctcggcc agctcctgtc gagccggcct
    10081 gacctggttt ccgcagacat catcgccgaa ctggtcaagc ttcaggatag ggtgcccccc
    10141 ttcccgaccg accaggcccg tcgaatcatt gaaaccgatc tgggaatgcc cctctcacaa
    10201 tgcttcgccg ggttcggtga cgagcctttt gcctgcggct ccatcgccca ggtttaccgg
    10261 gcaaccaccg ttgccgccga tgaacggccg tcctgccgcg tggttgtcaa ggtcaaacgg
    10321 ccggggatcg aggataccat ccggctcgac atgacgattc tcagatggat cggagacgtg
    10381 gccgagcggc tggtgcctga gctcgctgtc taccatccga aaatgatcgt cgatgaattc
    10441 gagcagacga tgatgcgcga attggatttc gtcaatgagg cggccacgat cgctcgcttc
    10501 gcggaatcca tcggcgagga tcccgatttc cgagtaccga cggtgttctg ggagtttacc
    10561 ggaccgagcg tgctgaccct tgaagaaatg cacggcgtca gcatccagac gctgctcacc
    10621 cgacctgatc cggccatcga ccgtaagcaa cttgccgacc ggctggctcg cgggttcgtc
    10681 aggcaattct tcgagaccgg actgttccat gccgatccgc accccggaaa cctgctggtc
    10741 gaaccgccgg ccgcgattcg cctgatcgat tttggtctga ccggccgaat cgacgaggaa
    10801 atgctcggac acctggtcat ggccctcatg ggagccgtca atcgcgagcc cgagatcgtg
    10861 gtcgaggtac tcgcggacat ggacgccttg ggcgagcaga ccgaccgagt acagctccgg
    10921 cgcgatttcc tcgagctgat tgagaaatac tacggtctgc cactgcaccg cttcgatatg
    10981 cagacgctgt tccatgagat cacggctctg atccggcgga acgatgttac ggtccctcgc
    11041 gagtttgtgc ttttcggcaa ggccctggtg gccgtcggtg ggatctgcct gcagctcgat
    11101 ccggaaatgg atctggtgac gctggtcaaa ccccggctgg tcaagctgct cgcagaacgt
    11161 atggccccgg gcaagctgct taaatccgcc gccatgtcag gctggcacct gctcaatttc
    11221 ctcaagggct tccccagcca gttgcgggac gtatcccggc gaatggcccg cggcaagtgg
    11281 caaatcaaca tccgccatca gaacctcgat ttcctggcca acgagatcga ccgagccagc
    11341 aaccgactga gcttcgccgt gatcacggcg gccatcatca tcggcagctc atgggtcttg
    11401 gcgtcctcgt cggccaccgc cgtcctcggc gtccccactt ggagtctcgg cgtcgtcggc
    11461 tacgtgttgg ccggcctcat gggtctgtgg ctggtcatcg ccattgttcg cagcggaaag
    11521 ttgtcctgaa cccaatcacc aaatcgcccc ggccggcgtc gtaaaccgac ccgatggcct
    11581 gaaatggtga ccttttccgg accaatccct gcccgcggcg acacgccggg cagggagggc
    11641 gaggttctcc ctgccggcct cgatccccgg ccccccactt cgtccgagaa cgcggcatcg
    11701 tccgggaaaa caccccctgg caggcaggag gccgcgccca ccccgccgat tttgtcatat
    11761 ttccgtttga ttttcgcccg aatgaatcct aaatatctca tggaagctgt cgtgtcccct
    11821 ttcaacggcc ccgcggtgcc cgggttgggt accgcggggt tatttttcgc cgggcaggcc
    11881 agtcgggcag gttccatgcc gaggcccgga gtgattgggc cggcacgtac gctgaatgac
    11941 gaagctggag tgccccccgt ccacgccggg gccgcacaag aaacgggtaa ccagccgcca
    12001 ggtgctgcac ccccgccatg gcggtcgcgg atcccgcttc cgcttgcttt gagacccttg
    12061 tcattcagcc tcttctcgct taaggtgaaa agtccaccgg ttcgacggct tggtgcgtcg
    12121 gaccgaggct ggaatcttat ggccagacat cggggcggct tgcgcgcccg tttctggatc
    12181 gcagtgcggt ttgggttgcg gcccgcaaag aggcggtccg cctcaggact ttgccgacgt
    12241 ggctcaatgg cagagcggcg ctttcgtaaa gcgcaggttg tgggttcgat tcccaccgtc
    12301 ggctttctca acccatttgc tgtaaaggtc ttacgcctca tcctggctca caacatgttg
    12361 gccggccatg cccgccctgc taacgtgacc gttgccaggc caggtcgctg aacgctcgct
    12421 tgccagcgcc ggccggttcc tcccccggat tgttggccgt ccgcccagcg gttagattgg
    12481 ctcggacaga agccccgaca tcgccgggtt tcgccgtgcg tttcgaacgg gtccgacctc
    12541 gcggtcggga aaggagcttg cctcatgaat cgttcagcca ggatcggtcg tcgcactctc
    12601 ctgaaggcga gcggcatgct gactggcggc atcctggggc ttccgtatct cgtctcccat
    12661 cgcgcgctcg cggccgccgg ccgtccgggt gcgaacgatc gcctgaccgt cgggcacatc
    12721 ggcgtcggtg gcatgggcac ctaccacctg cgaaacatgg tcgaaagaat gaaaaggggg
    12781 caggtcaatg tcgtcgccgt ctgcgatgtg gacgagagcc gcctagccga ggcatcccgg
    12841 aacgccgggc cgcaggccga tgtctaccgc gattatcgct acatcctgga gcgcaaggac
    12901 atcgacgtgg tcgtcatcgc aacgcccgac cactggcatg cggtgcagac ggttcacgcg
    12961 gccgaatgcg gcaagcacgt gtacgtcgag aagcccgctt gctgcaccat tgaagaaggc
    13021 aaggcaatgg tccgggccgc ccgcgaaaac agaatctccg tgcaggttgg ttcacagggt
    13081 cgctccgagc acgaagcata ccttgctcat cggtacctgg tgaacgacaa catcggtcgc
    13141 atcgacaggg tctggtgttt ccactacccg tcaccgtccg atgacaatcc cgttcccgac
    13201 agcgagccgc cgcctgaact cgactgggac ctgtggctgg gcccgatgcg ctg
//
LOCUS       DAOTPW010000159        13160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4564_length_13160_cov_5.586417, whole genome shotgun sequence.
ACCESSION   DAOTPW010000159 DAOTPW010000000
VERSION     DAOTPW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4564_length_13160_cov_5.586417"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            561..1946
                     /locus_tag="P5316_14325"
     CDS             561..1946
                     /locus_tag="P5316_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86179.1"
                     /translation="MAVARPQGSVGSSGLKAAAIGFAVAAVAAITFTIIMFTQKSDLE
                     ATASEAQRQARDQQRAAADARQALSDVAQEVIGKPAESLAEIKSAIDEIRTTVANDPQ
                     VKDAKITADQPVATMLNNLFSLYQKTAGRADELQAQHKKLNNDLEQAVKTRDDAAKAF
                     ETRVAELDEKFKKLMQESEQSREECNRQLAELQAARTKERDQMAGELDAARKARDEAE
                     KTLNAEKNRTEGLLAQLAQFKPSSSGSALQVKDGVIVRTVPAGDIVYIDLGRSDGVRL
                     GMTFSVYSPVRGIPTDGKGKATIEVVNVFETTAECRVTSRTPGEPILVNDIIANPVFD
                     RSRQFTFAVIGDFDLNFDGRPEDIAGKEVSRLIDRLGGKVVNKVDTDTDFLVVGSPPT
                     GPVPVIPGSEDEAAVTERNEQAAKRLSAFETMLKEARSLSIPILTRTQFLHFLGQSMP
                     ANTPDDTLPTL"
     gene            2373..3875
                     /locus_tag="P5316_14330"
     CDS             2373..3875
                     /locus_tag="P5316_14330"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRR86180.1"
                     /translation="MTVHIRDLGKHIDSTVTLAGWLYNSRVSSKVVFLVVRDGTGLCQ
                     CVVGKQDVPPEVFETASRLGQESSLKVTGQVKAEQRAPGGHELHVSGIEVVHSTTDYP
                     ITPKPHGHEFLFKHRHLWLRSRRPTAIMKIRHTVIDAIRRFFNDNGFTLVDTPIFAPS
                     AGEGAQTLFSVDYFGEPMYLAQTGQLYLEAAALAYGKVYCFGPTFRAEKSKTRRHLTE
                     FWMVEPEIAYAVLEDVMDVAEDFVCSIVQRVLKEHRADLEFLGQDLAALENVKQPFYR
                     ITYSQAAEILRGPKARELLEKDLAEKSARITQLKESIPAKEAARDRQGVKKHEQEKLA
                     AEVIAEREELAELEEQVSNIPKHMELAANFEWGKDLGGSDETIISRLHDRPVFVTHYP
                     RGCKAFYMKRDHEDPRVVRNFDLLAPDGYGEIIGGSQREDDLNVLLERMAEEGLATGP
                     YEWYLDLRRYGSVPHGGFGLGVERTVAWICGLKHIRETIAYPRMMDKVYP"
     gene            complement(3866..4057)
                     /locus_tag="P5316_14335"
     CDS             complement(3866..4057)
                     /locus_tag="P5316_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015222549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRR86181.1"
                     /translation="MLYKVPLLLTPQPEGGFTVTSPLLPELAAEGDTADEALGNVRDA
                     LAAVIEIYHDLGRPLPAYG"
     gene            4169..4369
                     /locus_tag="P5316_14340"
     CDS             4169..4369
                     /locus_tag="P5316_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86182.1"
                     /translation="MSLIPNPAELRRRGLEILVRELGYVNAMRLLLQYETGQGDYTRD
                     RDQILSDWSVTEMVREADKLVR"
     gene            complement(4456..4695)
                     /locus_tag="P5316_14345"
     CDS             complement(4456..4695)
                     /locus_tag="P5316_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86183.1"
                     /translation="MLTVCRKLLHLCKGDTPYYSKCGYNLTDLTSPRCPECGGLPSEV
                     PVVRGELAIGRSLRIWLVVGMLLMLVIGVAAAIVW"
     gene            complement(4899..7505)
                     /locus_tag="P5316_14350"
     CDS             complement(4899..7505)
                     /locus_tag="P5316_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRR86184.1"
                     /translation="MSNRSQGVMFPCPLAVIIISTAMLLAVASAFGESPARVNVSTFY
                     HAQARANAQTNGQRYDWARKQVDEAIAAAEPWLKTPDDELWRMIVPPKLKRAIMVNVN
                     QGCPKCGMGIYGDRGPHPYPWLAWVEGHPWKVQCPNCKELFPKNDFGAFYESGIDPET
                     GLFDPARADRKLLFNAEHADPRDPQHLYAVDDGSGWQRFPGGLKERDWYIGYHAYFGR
                     SGRVASAIRSLATGYALTGRPIYAHKATILLDRLADVFPDYDGKREQFYNNVWGEYSD
                     GLLGPNYWDGGMWAQRAIDYDMIREGIGQAPETLTFLGEKARQFKVPMPKTSVEDIRR
                     NIEQRILIDGGRRRERIWMNGTITEMCRLKVELVLNGRDALKELVGKYMPAIVPPEHL
                     NADGSGNERSTGYDAGAFGQYCSLIEELAAMDKDVVRAALESHPNLRAAFDFWPDIWC
                     LEKYIPQIGDVGEPGATSGPRGSAGAYLVLFDLTGNARYAQAAMRIANGDASALPRNI
                     YAAEPEELVQRAIEADKTSGPWHTPSIVKRDYQLGILRSGLGPDEQALWLFYSPKAGT
                     SSHSHFDALNIGLFAFGLPLICEQGYPLYTGDWPARWAWTSNTRSHATVSVDNRCQRH
                     SDGGKLLAFAGDNGVQLISAEAPTAYENVAVYRRTLVAAELGAGCVCYLDVFRIKGGR
                     EHTYNLPLFYGNLRVQGLTMIPHDDLHDGYVSQVQASPAESPWQAEADLCEEYEGLVR
                     AHMRIHGPAADMTILTGRGEARWGKDDPRRLPYLFLRHQTNARELDSCFALVYEPYRE
                     RPFIDEGSVVIRPEAQAVHFEARLADGSGSFRLTCRDMGDSPVTAEGTRTLAGKSQDV
                     KLSGIISTLPSR"
     gene            complement(7539..8654)
                     /gene="nadA"
                     /locus_tag="P5316_14355"
     CDS             complement(7539..8654)
                     /gene="nadA"
                     /locus_tag="P5316_14355"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRR86185.1"
                     /translation="MPYQPPLPPQYTDLSIEEMCERIRAHKQAFGRRLVILGHHYQQD
                     DVIQFADFTGDSLKLSRQASQQKDAEFIVFCGVHFMAESADILSEGRVAVILPDLTAG
                     CSMADMAALEQVEDAWQRLTAATDAKVIPITYVNSSAAIKAFCGQHDGACCTSSNARK
                     VLEYALTQGDKVLFLPDQHLGRNTAYAMGHPLDSMVLYDPVLDAGGLTDEQIRNARFL
                     LWNGYCSVHQVFTPQQCERVRAADPACKIIVHPECPWEVVQRADLAGSTEYIIKIITE
                     APEGSHWAVGTEINLVNRVANRYAGRKLVRSLADVQCLCSTMYRIDLPHLLWSLDELA
                     TGRVVNQIRVDEQTRHFARVALQRMLDNVSATPMAAK"
     gene            8994..11534
                     /locus_tag="P5316_14360"
     CDS             8994..11534
                     /locus_tag="P5316_14360"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HRR86186.1"
                     /translation="MIAPWYKGLNGQCDFRVRVAAETLKRYPWATNPKTGLPAPDYIY
                     NGRWEIKPDGTIRPRKLEDWGNGDLAQRGAYAMIGWVDYYRYSGDPSAIAQISMIADV
                     LLNHCQTTDDHPWPRFLISVPNQGTPYGECNPQGMIQLDLVALFGLGLVRGYQITGNR
                     EWLDTAGHWADLLAAKRGHEPGLSPWNRYANPEDVFWDDRQTGGVVVLLRLFDELIRV
                     GYTGVNNALIEARDAGRAYLRDVLLPRWLENDAFGRDYWDWSHPVQSETLSEMVPRYL
                     MAVPDVFRNWRVDARNIMSLFLQRACVAPESNGDVYSGAWAYPEGAQCCGRSLSYPPM
                     QVGAAFAEYGVRADSEWARELARRQFILATYDAHETGVVEDGMEGGNVVAGNWLQIAH
                     PLPLAYVLDAMAWLPDVLGPHRENHIMRSTSVVSKVIYGDGRIEYTTFDAPAGTEDVL
                     RLAFRPTRILADGKPLSESPSLDGNGYAARPLNNGDCIVTIRHDGSRTVIIEGDDPQQ
                     QSDERTMSFSGRWDEASLAGHFGGAVRLTAEAGAELSFTFEGNQFRLIGAVSPNGGLA
                     NVYIDDVKQLCGIDCWNPYVLRQQVLYHKSGLSNDRHTIRIVAFGKGNPRSNGADVTA
                     DAVQFSAATGDAGFGEGGGPTDAQRWVFGYPGREDYVDSQGRSWRPATEAVIRVGELT
                     DPVEACWYTTPRRLTVAGTADPVLYRHGMHGRDFTAYVTVGPGDYHVRLKLMESRWVT
                     PETRLMSIDINGRPMLRNLDIAATATGRPASVAFTANNRPIWDGLNRAVDLVFDDIRP
                     NHGVIEVRFRGVNDAEAVVSAMEIAPGRGGLGATPILALTPSTTQAAN"
     gene            complement(11614..12192)
                     /locus_tag="P5316_14365"
     CDS             complement(11614..12192)
                     /locus_tag="P5316_14365"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRR86187.1"
                     /translation="MAGLMDWLNSHPLVLPLFIFFARITDVSIGTLRTICIVRGFRYL
                     APILGFFEVAIWIIAVSGVFSHLDRWYNIVAYAGGFAAGNAVGMYIEQQLAIGMQAIR
                     IISPGHSAAVAAGLRLAGYGVTEIKGRGLNGEVSICFVVVPRKETPVVLRVARSIDPD
                     AFSTVEDVSSAVGRVYRDEVRKRGWRNLLKRK"
BASE COUNT         2658 a         4145 c         3979 g         2378 t
ORIGIN      
        1 gactggttca cggtcggcca attcctcctt ctctgtgtta ccccatcaag cccgttttaa
       61 cgggcctgtc cttgtcaggt aatcctgttg aaacgggctg agaccataga aaggaagaag
      121 aaccgcgggc gaccgccaac cggccgcaca acggccggcc tatcagcccg ccttcggcgg
      181 gaaaacccgc tgaagggcct tgatcgccca acctggactg gtcggcatgc ccgcccagac
      241 tgtcacgcac ccgggcggca cctggtcgac cacgagacat tgacaggccc atgcccggaa
      301 acgagaatga cggattggct acccgcctga gcaggtggcc gggcggcagt cgaggcggct
      361 gccgttctgc cggcactgag accgaaggtg gttccctcct cgaaatcgtc cgtacgccga
      421 gatgaaagcc ggctggatcg acttccctcg gaccacggtg ccgacaatcg aagcgggcga
      481 acctcgcggc ccgtccgagc ccgaccggac ggcatggaca cccgacgtcg ggcctaacgg
      541 tcgaaacgga gcagtgaatc atggcagtgg ccaggcccca aggatcggtc ggatcgagcg
      601 gcttgaaagc agcggcgatc ggttttgccg tcgcagcggt ggccgccatt accttcacca
      661 tcatcatgtt tacgcaaaag tcggacctcg aggcaaccgc cagcgaagcc caacggcagg
      721 cccgcgatca gcaaagggcc gccgctgacg cccgacaagc cttgagcgac gtcgcccagg
      781 aggtcatcgg aaaacctgcc gagtcgctgg ccgagatcaa gtcggcgatt gacgagatcc
      841 ggacaaccgt ggccaatgac ccgcaggtca aggatgcgaa aatcaccgcc gaccagccgg
      901 tggccacgat gctgaacaat cttttcagcc tgtatcaaaa aaccgccggc cgggccgacg
      961 aacttcaggc tcagcacaag aagctcaaca acgatcttga gcaggccgtc aagaccagag
     1021 acgatgccgc caaggccttc gagacccgag ttgccgaact ggacgagaag ttcaagaagt
     1081 tgatgcaaga gagcgaacag tcccgtgagg agtgcaaccg acagcttgcc gaactccagg
     1141 ctgcccgcac caaagaacgc gaccagatgg ccggtgaact tgatgccgcc cgcaaagccc
     1201 gggatgaggc cgagaagacc ttgaatgccg agaaaaaccg gacggagggg ctgctggccc
     1261 agttagcgca attcaagccc tccagcagcg gctcagccct ccaggtcaag gacggcgtga
     1321 tcgtccgcac agtgccggcc ggggacatcg tttacattga cctcggccgc agtgatgggg
     1381 tcaggctggg catgaccttc tcggtctatt cccctgttcg cggcatcccc actgacggaa
     1441 agggcaaggc aaccatcgag gtcgtcaacg tattcgagac gacggcagaa tgtcgcgtga
     1501 cctcacgcac gcccggcgag ccgatcttgg taaacgatat tatcgccaac cctgttttcg
     1561 atcggtcgcg ccagttcacc ttcgcggtca tcggggactt cgatttgaac tttgacggtc
     1621 gccccgaaga catagccggc aaggaagtga gtcggctgat cgaccgcctt ggcggcaagg
     1681 tcgtgaacaa ggtcgatacc gatacggatt tcctggttgt gggttcacct ccgaccggcc
     1741 ctgtcccggt catccccggc tcggaagacg aggcggccgt caccgagcgc aatgagcaag
     1801 cggccaaacg cctatcggct ttcgagacca tgctcaagga agctcggtcg ctgtcgatcc
     1861 cgatcctgac gcgcacccag tttctgcatt tcctgggaca gagtatgcct gccaacaccc
     1921 cggacgacac gttgccgacg ttgtaggccg tcgcgttaac ccgaagaatt gcccgctggc
     1981 gatcttgaaa aaccggtctt gacataatct gcgaatactc cctgaacgcg tcggtcacat
     2041 ggggcaacaa tgaccaccgg ctgcctgtaa gccggacacg gcgatggcat ggggcgaggc
     2101 gatgggtgaa tcgggggcgg gatcggaacc gttgggactc gcagtgaaga cgggcaaatc
     2161 gatgccgaac gcggaacgat caacattgtg ccggcgcggc ggtttctcct caacttgcgc
     2221 aattcgacgg agcaggcgtc gcaggagtca gcctctctca agatctgaat ccggatctgc
     2281 cttgccccct tttgctgacg gtgccgctcg cgtatcatcc cccggcatgc gactgaggaa
     2341 ggctttcggc gaacaggcac aaggaaacag acatgactgt tcatattcgg gatctcggca
     2401 aacacattga ctcgacggtg acgctggccg gctggctcta taactcgcgg gtcagcagta
     2461 aggtggtttt tcttgtcgtc cgcgacggta cgggcctgtg ccagtgcgtg gtcggaaagc
     2521 aggatgtgcc gccggaggtc ttcgaaacgg cctcacggct ggggcaggaa tcttcgctca
     2581 aggtcaccgg ccaagtcaag gccgagcagc gcgctcccgg cggccacgaa ctgcacgtca
     2641 gcggcatcga ggtggtgcac agcacgaccg actaccccat cacccccaaa ccgcacgggc
     2701 atgagttttt gttcaagcat cgccacctgt ggctgcgatc ccgacgcccg acggccatta
     2761 tgaagatccg tcacacggtc atcgatgcca tccgccgctt ctttaacgac aacggcttca
     2821 cgctcgtgga cacgccgatt ttcgccccct cggccggcga aggagctcag accctcttct
     2881 cggtggacta ctttggcgag ccgatgtacc tggcccagac cggccaactc tatctcgagg
     2941 cggcggcctt ggcctacggc aaggtctact gcttcggccc tacttttcgg gcggagaagt
     3001 ccaagacgcg ccggcacctg acggagttct ggatggttga gccggagatc gcgtacgctg
     3061 tcctcgagga tgtcatggat gtcgcagagg attttgtctg ctcgattgtc caacgagtgc
     3121 tgaaggaaca ccgcgcggac ttggaattcc tcggccaaga ccttgccgcg ctcgaaaacg
     3181 tgaagcagcc gttttaccgc atcacctaca gtcaggcggc cgagatcctt cgcggcccga
     3241 aggcccgcga actcctggag aaggacctgg ccgaaaagag cgcccgcatc acccagctca
     3301 aagagtcgat cccggccaag gaagcagccc gcgatcggca gggcgtcaag aagcatgagc
     3361 aggagaaact cgcggccgag gtcatcgcgg agcgggaaga actggccgaa ctggaagagc
     3421 aggtctcgaa catacccaaa cacatggaac tggcggcgaa cttcgagtgg ggaaaggacc
     3481 tcggcggcag cgacgaaacg atcatctcgc ggctgcatga ccgtccggtg ttcgtcacgc
     3541 actatccgcg cggctgcaag gcgttttata tgaagcgaga tcacgaagac ccgcgggttg
     3601 tccgcaactt cgacctgctg gcccccgacg gctacggcga gatcatcggc ggctcacagc
     3661 gcgaggatga cctgaacgtg ctgctggagc gcatggccga ggaagggctg gccaccgggc
     3721 cctacgagtg gtacctcgac cttcgccgct acggcagcgt cccccacggc ggcttcggcc
     3781 tcggcgtcga acgaaccgtc gcctggatct gcggcctcaa acacatccgc gaaaccatcg
     3841 cctatccgcg aatgatggac aaggtctatc cgtaggcagg caatggccgg cccagatcat
     3901 ggtaaatctc gataactgcg gcgagcgcat cccgaacatt cccgagcgct tcatcggcag
     3961 tgtcgccctc cgccgccaat tccggcagga gcggcgaagt aaccgtgaaa cccccttctg
     4021 gctgaggggt gaggaggagg ggcactttgt agagcatgtg tcaccaaccc ttcgctgttc
     4081 cgtaacgctt tgacctgcca tattacaccg taccgagtct cgagaagaca gaagtcgact
     4141 tgatgacgtt gacgagagag ttgcccgcgt gagcctgatc cccaatcctg ctgaactgcg
     4201 acgtcgaggc ctggagatcc tcgtccgcga gctgggctac gtcaacgcca tgcgtttgct
     4261 cctgcaatac gaaaccggcc agggcgacta cacccgcgat cgggatcaga tcctgtcgga
     4321 ctggtccgtc acggaaatgg tccgcgaggc ggacaagctg gtccgctgag cgaacgtctc
     4381 cctcgtagcc aaatgcccgc attgcgtgtc cctctcactc atcgcacggt cgtaaccgcg
     4441 agatgctggg tctgatcacc atacgatggc agcggcgacg ccgataacca gcatcaacag
     4501 catcccgacg accagccata tgcgcaggga ccgccctatg gccaactccc ctcgcaccac
     4561 gggcacctcg gatggaagac ctccacactc gggacatcgc ggagacgtca ggtccgtcaa
     4621 gttgtagccg cacttgctat aataaggcgt gtcacctttg cagaggtgca gcagcttgcg
     4681 acagacggtg agcatcgaaa ccgaggcgat caagatgcag acaactgtga gcgtcggttc
     4741 acctggcgct caccttcgcg atattcattc catccggccg ctcagttcag cctgcccagt
     4801 ttgaccgtat cgaagaggat caggttccac ttcttgcccc tgcgcatagc gttattgaga
     4861 aagaccctac tttcagaatg cggaaccatt aaattggctc agcgcgacgg cagagttgag
     4921 atgattcccg agagcttgac gtcttgcgac ttgcccgcca gcgtgcgtgt gccctccgcc
     4981 gtcaccggcg aatcgcccat gtcccgacag gtcagtcgga acgaacctga cccgtcggcc
     5041 agccgggcct cgaaatgaac ggcctgtgcc tccggtcgta tgaccacgct gccttcatcg
     5101 atgaacggcc gctcgcgata cggctcgtaa acgagagcaa agcagctatc gagttcgcga
     5161 gcgttcgtct ggtgacgcaa gaacaggtat ggcagtcgcc gcggatcatc cttgccccaa
     5221 cgcgcctccc cccggccggt caggatcgtc atgtcagcgg ctggaccgtg aatgcgcatg
     5281 tgcgcacgga ccagcccttc gtattcctcg cagagatctg cttcggcttg ccacggcgac
     5341 tcggccggcg aagcctgcac ctggctcaca tagccgtcgt gcagatcgtc gtgcggtatc
     5401 atcgtgagtc cctgaacgcg cagattgccg tagaacagcg gcaggttgta cgtatgttca
     5461 cggccgccct taatgcggaa gacgtccagg tagcagacac atccggcacc aagttccgcc
     5521 gcgaccagcg tcctgcgata gaccgcaaca ttctcataag ccgtcggggc ctcggccgag
     5581 atgagttgca cgccgttgtc tccggcaaag gccaagagct tgccgccgtc gctatgacgc
     5641 tggcagcggt tgtccacgct gacggtcgca tgactgcggg tgttgctggt ccacgcccag
     5701 cgggccggcc agtcgccggt gtagagcggg taaccctgtt cgcagatcag tggaaggcca
     5761 aaggcgaaca aacctatgtt cagcgcgtcg aagtgcgaat ggctgctggt tccggccttg
     5821 ggggagtaaa acagccatag cgcttgctcg tccggaccga ggccgctgcg caaaatgcca
     5881 agttgatagt cccgcttgac gatgctgggc gtgtgccacg gcccggatgt tttgtcagcc
     5941 tcgatcgccc gctgaaccaa ttcctcgggc tctgcggcgt agatgttgcg aggaagcgcg
     6001 gaggcgtcac cgtttgcgat gcgcatggcc gcctgggcat acctggcgtt gccagtcaga
     6061 tcgaaaagca ccaggtaggc acccgccgag ccgcgcggcc cgctggtcgc gccgggttcg
     6121 cccacgtcgc cgatctgggg gatgtacttc tccagacacc agatgtcggg ccagaaatcg
     6181 aaggcagcgc ggagattcgg gtgactctcc agggcagcgc gtacgacgtc tttgtccatc
     6241 gccgccagtt cttcgatcag gctgcagtat tggccaaaag caccggcgtc atagccggtg
     6301 gaacgctcat tccccgaacc gtccgcattg aggtgctcag gcggtacgat ggcgggcatg
     6361 tacttgccga ccagttcctt cagcgcgtca cgtccgttga gcaccagctc aaccttgagc
     6421 cggcacattt cggtgatcgt gccgttcatc cagatacgtt cgcggcggcg gccgccgtcg
     6481 ataaggatgc gctgctcgat gttgcggcgg atgtcttcga cactggtctt gggcatcggc
     6541 accttgaact ggcgggcctt ctcgcccagg aacgtgagcg tctcaggcgc ctggccgatg
     6601 ccctcacgaa tcatgtcata atcgatcgcc cgctgagccc acatgccgcc atcccagtag
     6661 ttcggcccca gcaagccgtc cgaatactcg ccccaaacgt tgttgtagaa ctgctcccgc
     6721 ttgccgtcgt agtcggggaa cacatcagcc aggcggtcga gcaggatggt cgccttgtgc
     6781 gcgtagatcg gccggccggt gagagcgtag cccgtggcca gcgagcggat cgccgacgcc
     6841 acccggccgc tgcgcccgaa gtaagcatga tagccgatgt accagtcccg ctccttcagg
     6901 ccaccgggaa agcgctgcca gccgctaccg tcatcgacgg cgtacaagtg ctgcggatcc
     6961 ctcggatcag catgctccgc gttgaacagc agcttgcggt cagcccgcgc cggatcgaac
     7021 aagcctgttt caggatcaat tccactttcg taaaacgccc cgaagtcgtt cttcggaaac
     7081 agctctttgc agttagggca ctgtactttc cacggatggc cttcgaccca cgccagccag
     7141 ggatacggat gcggtccgcg gtcaccatag atgcccatgc cgcatttcgg gcaaccctgg
     7201 ttgacgttga ccataatcgc ccgcttgagc tttggcggca cgatcatccg ccacaactcg
     7261 tcatcgggcg tcttgagcca cggctcggcc gccgcaatcg cctcatcaac ctgcttgcgg
     7321 gcccaatcgt atcgttggcc gtttgtctgg gcgttggctc ttgcctgggc atggtagaac
     7381 gtggatacat tgactcttgc gggcgactcg ccgaatgccg acgccaccgc gagcaacatg
     7441 gcagttgata tgatgatcac agccaacgga caagggaaca tcacgccctg cgatcgattg
     7501 ctcatctcca gtccccagcc tccaatcccc cgtccccact atttcgccgc catcggcgtg
     7561 gcgctgacgt tgtcgagcat acgctgaagg gcgactcggg cgaaatggcg ggtctgctca
     7621 tcgacccgaa tctgattgac cacgcggccg gtggccagct cgtcgaggct ccacaacagg
     7681 tgcggcagat cgatgcgata catggtcgaa cacagacatt gtacgtcggc cagcgaccgc
     7741 accagcttgc gaccggcata gcggttggcc acgcggttaa ccaggttgat ctccgtgcct
     7801 accgcccagt gcgagccctc gggggcctcg gtaatgatct tgatgatgta ttcggtgctt
     7861 ccggccaggt ccgcccgctg caccacttcc cacgggcact ccggatggac gatgatcttg
     7921 caggccggat cggctgcccg aacacgctcg cactgctgcg gcgtgaacac ctggtgcacg
     7981 ctgcagtagc cgttccacaa caggaagcgg gcattgcgga tctgctcgtc ggtcaggccc
     8041 ccggcgtcca ggaccggatc gtaaagaacc attgaatcga gtggatgccc catcgcgtac
     8101 gccgtgttgc ggccgagatg ctggtccggc aggaaaagga ccttgtcgcc ctgcgtcaac
     8161 gcatattcga gcaccttgcg agcattggaa ctggtgcaac aggctccgtc atgttgtccg
     8221 cagaacgcct tgatcgccgc cgagctgttc acgtacgtga tggggatgac ctttgcgtcc
     8281 gtcgccgcag tcaaacgctg ccacgcgtcc tcgacctgct ccagcgctgc catgtccgcc
     8341 atactgcacc cggcggtcag gtcgggaaga atcacagcca cccggccttc cgacagaatg
     8401 tccgctgact cggccatgaa atgcacgccg cagaagacga tgaactcggc gtccttctgc
     8461 tgcgaggcct gccggctgag cttgaggctg tcgccagtga aatcggcgaa ctgaatcacg
     8521 tcgtcctgct gataatgatg gccgagaatc accagacgcc ggccgaaggc ctgcttgtgc
     8581 gcccggatcc gctcgcacat ctcctcgatc gagaggtcgg tatattgagg cggcaatggc
     8641 ggctgatacg gcatcacgtc aggtcggctc ctatacacca ggtgaaccag actctgaatc
     8701 agtatagcct cgcggctttc gtggacaaaa cctgacggct gtccggctcg gcgtgacagg
     8761 ctccaagccc tggctgtacg atatgccgga tgtcgcctcc gcaggtgttg tacggcgcga
     8821 cgtcccaagc cgaagcgcag ggagagcact gcatgaagca ctctcaagaa gcatctgcga
     8881 aggcggttat atccctacca cttctatttt gcctcgtatt ccccgcctgg gcgtcggcgc
     8941 agcgaacgca gccgcgatac ttcgcacatg atgccgtcga ggatcgccat ggcgtgatcg
     9001 ctccgtggta caagggcctc aacggtcagt gcgatttccg ggtgcgcgtc gccgccgaaa
     9061 cgctgaaacg ctatccgtgg gcgacgaatc ccaagaccgg cctgccggcg ccggactaca
     9121 tttacaacgg ccgttgggag atcaagccgg acggcacaat caggccgcgc aagctggagg
     9181 actggggcaa cggcgacctg gctcagcgcg gggcatatgc catgatcgga tgggtggatt
     9241 actaccgcta cagcggcgac ccgtccgcga ttgcccagat cagcatgatc gccgacgtgc
     9301 tgttgaacca ttgtcagacg actgacgatc acccctggcc gaggttcctg atcagtgtgc
     9361 ccaaccaggg caccccctac ggcgaatgca acccgcaagg catgatccag ctcgacctcg
     9421 ttgccctgtt cggcctcggt ctggtccgcg gctaccagat caccggcaac cgcgaatggc
     9481 tcgacaccgc cgggcattgg gcagacctgc tggcggctaa acgaggccac gaacccggcc
     9541 tgtctccgtg gaaccgatat gccaatccgg aggacgtgtt ctgggatgac aggcagaccg
     9601 gcggcgtcgt ggtcctgctg cggctctttg acgagctgat ccgcgtcggt tacacgggcg
     9661 tgaacaacgc cctgatcgaa gctcgcgatg ccggccgggc gtaccttcgg gacgtgctgc
     9721 tgccgcgatg gctcgaaaac gacgcttttg gccgggatta ctgggactgg tctcaccccg
     9781 tgcagagcga gacgttgtcg gagatggttc caagatacct gatggccgtg ccggatgtct
     9841 tccggaactg gcgggttgat gcccggaaca tcatgtcgct gttcctgcag cgtgcgtgcg
     9901 tggcccccga gtcgaacgga gacgtctaca gcggagcctg ggcgtatccg gagggggctc
     9961 aatgttgcgg gcgttcgctg tcgtacccgc cgatgcaggt tggggccgcg tttgcggagt
    10021 acggcgttcg ggccgacagc gaatgggcac gcgagctggc ccggcgacag ttcattctgg
    10081 caacgtacga tgcccatgaa accggcgtgg tcgaggacgg tatggagggc ggtaacgtcg
    10141 tcgccggaaa ttggctgcaa atcgcgcacc cgctgccgct ggcttatgtt ctggatgcca
    10201 tggcgtggct gccggatgta ctcggtcccc accgcgagaa ccacatcatg cggagcacgt
    10261 cggttgtgtc gaaagtaatc tacggcgacg gccggatcga gtacaccacc ttcgatgctc
    10321 ctgcagggac ggaagatgtt cttcggctgg cgtttcgccc gacgcggatc ctggccgacg
    10381 gcaagccgct ttctgaatcg ccttcgctcg acggaaatgg ctatgctgcc cggccgctga
    10441 acaacggcga ctgcatcgtc acgattcgac acgacggcag caggacggtg ataatcgagg
    10501 gtgatgatcc gcaacaacag agcgacgaac gcacgatgtc attctccggc aggtgggacg
    10561 aggcgagtct ggccgggcat ttcggcggcg cggtccgtct caccgcggag gccggcgcag
    10621 agctctcgtt cacgttcgag ggcaaccagt tccgtctgat cggggcggtg agccccaacg
    10681 gcggcctggc caacgtttac attgatgacg tcaagcagct ctgcgggatt gactgctgga
    10741 acccatatgt tcttcggcag caggtgttgt accacaagag cggcctgagc aacgatcggc
    10801 atacgatcag aatcgtcgca ttcgggaagg gcaatcctcg ctcgaatggc gccgacgtca
    10861 ccgccgacgc agtccagttc tccgcggcga ccggcgatgc gggtttcggc gaaggaggcg
    10921 gtccgaccga cgcgcagcga tgggtattcg gttatcctgg ccgtgaggac tatgtcgatt
    10981 cacagggccg ctcgtggcgg cctgccaccg aggcggtcat tcgtgtgggc gaactgaccg
    11041 atcccgtcga ggcctgctgg tacacaacgc cgcgacgact gacagttgcc ggtactgccg
    11101 atccggtttt gtatcgacac ggcatgcacg gccgggactt caccgcctat gtgaccgtcg
    11161 ggccgggtga ctatcacgtc cgcttgaaac tgatggagtc cagatgggtg accccagaga
    11221 ctcgtttgat gagcatcgac attaacggcc gcccgatgct tcgcaatctg gacatcgcgg
    11281 ctacagccac aggacggccg gcgtccgtcg ccttcaccgc aaacaacagg ccgatctggg
    11341 acggcctgaa ccgtgcggtt gacctcgtgt ttgatgatat ccgcccaaac cacggggtga
    11401 tcgaggtccg tttccgcggc gtcaacgacg ctgaagccgt ggtatctgca atggaaatcg
    11461 ccccgggacg cggcggactc ggcgccaccc cgatcctcgc gctgacacca tcgacgacac
    11521 aggctgccaa ttgaccgatt acttgcggaa gacgcggccc tggcggcgag cctccacccc
    11581 ggccgggtta cgaaggcata cccgcaacat ttactacttg cgtttcaaca ggtttcgcca
    11641 tccacgtttg cggacctcgt cgcggtagac ccgcccgacg gccgaagaga cgtcttcaac
    11701 cgtactgaat gcgtcgggat cgatgcttcg tgccacccga agcaccacgg gggtttcctt
    11761 gcgaggaacg accacgaaac agatggacac ctcgccattc aggccgcggc ccttgatctc
    11821 ggtgacacca taacccgcga ggcgcaaacc ggcggcaacc gccgcactat ggcccggcga
    11881 gatgatacga atcgcctgca tgccgatggc cagttgttgc tcaatgtaca tgcccaccgc
    11941 attgcccgcg gcaaaaccac cggcataagc cacaatattg taccaacggt ccagatggct
    12001 gaagacgccc gagaccgcga tgatccagat cgcgacctca aagaagccga gtatcggcgc
    12061 caggtaccga aagccgcgca caatgcagat ggtccgcaac gtgccaatcg acacatccgt
    12121 gatccgcgcg aagaagatga acagcggcaa caccagcgga tgagagttga gccagtccat
    12181 caagccggcc atgtttcatg accccagagc cgcgggcggt acggatggcc cgtccagctc
    12241 gcaggcaaaa cgccattcta ctcttgaggc gcttgccaat cgagaaacac atctctgcca
    12301 accaagggcg gtatctgaca accctttggc gatgagccgg gtggtctcac caggcctgcg
    12361 aatcccgcct ggaacgacac cggttcacca tccaccggac gtcgcgatta atgaagtcga
    12421 ctttgatgcc ggcaatgccc ctttcctcgt ggagcggaaa ggccttggcc atctccgcat
    12481 cgcagtgact ccacctcagc cacaaccaga cgccaacgcc cttctcgccg tggtgctcga
    12541 tgatcgcccg cccggcagcc gaggaggatc gcgcgccaag gagtctcgaa aggtgccacc
    12601 cgaaccacgg cccgctcgtt gcctcgcgtc gataaccgcg tcagcagggt cttgggcatc
    12661 cgcggactcg tcgcaagaca gagtccggca tggtcctgaa ggtgtgcctc agtaatcgcc
    12721 atgcactcgc cgcaagacat ctccggcgtc attggcgggc cgataagcgt ctcgccgagc
    12781 tttgacagcg acccgatcgt gtaagggctg tcgaacggca aggcctgccc gtccatcgtc
    12841 aagtcaaagg ccgggccctg aggaccggtt gaaagcgaaa aagacatctt tccatccggc
    12901 gactgcatcg ttaccggctc ggtcgctgca gccggacagg tcacaagtgc ccacgcgacg
    12961 gtggtttgaa ccaaacgcat gtcgggtctt ccctcgctgg gagaagtaga gcggtccgcc
    13021 ggcaagatgt caacgtcgaa agcgatgacc accggcagcg ttcaataggt cgtatcactc
    13081 ctgtccgggt aagacggccc gattatgtat caaatgcctg attctcaagg attccctcgc
    13141 cgcaggaggg ggtacacgat
//
LOCUS       DAOTPW010000160        13134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4581_length_13134_cov_5.111706, whole genome shotgun sequence.
ACCESSION   DAOTPW010000160 DAOTPW010000000
VERSION     DAOTPW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13134
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4581_length_13134_cov_5.111706"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="P5316_14370"
     CDS             complement(<1..1170)
                     /locus_tag="P5316_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86188.1"
                     /translation="MSSDRAISRRGLIRRAAAGLAAPYFVRASALGKDGTTAPSNRII
                     YGLIGCGPHGVDWNLVQIFRYSDAQVIAVCDVDEARCLTGKARVDEHYAESLGRDNYK
                     ACATYGDFRELINRKDIDVIANCTPDHWHVIPAIMAAKAGKDVICEKPLTLTVAEGKA
                     LCEVIKQTGRIFQTASENRSIDSYIHMCELVRNGRIGKLRHIRVSLPAGNETRGENFN
                     DRQVQPPPKGFNYEMWQGQAPEAPYCPARCHGSFRWNLAYSGGRLTDWGAHMIDLAQW
                     GNGTENTGPIEVEGTGKFPPRDALFNTAEEFDLRYRYANGVTLNVVSKGPGIRFEGTD
                     GWIGFEGWRAPLQAGDPKILASKIGPNEIRLYRPSEVVPRTEGMKGGEHRNFIDCV"
     gene            1719..3581
                     /locus_tag="P5316_14375"
     CDS             1719..3581
                     /locus_tag="P5316_14375"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HRR86189.1"
                     /translation="MMMVTENDILERLGDLLAELAGLPVKPVRQGEKADLRLRLGSHL
                     LAVEVKTNSRAGVVAQAAENARQAADSGKPTAIPLVAVPFMGEVGREICRECGVSYVD
                     MSGNGDINAPGLRIHVAGKPNLFVQQGRPSSVFAPKSSRLARILLAEPKRWWRQAELA
                     SAGQLGAGYVSRICRRLEEDHLIERDSEGSVRPRDPNLLLEAWQAQYDLRRHDIRQGH
                     VAARSGEELARQVAEACRGLGFDYALTGLAAAWLLAPFAGFRLVTVYLRKSPNDKLLQ
                     RLKWHEEKQGANLWLIRPNDEGVFHGAQNVKSTVCVSPVQALSGSAQPAGTGRGSGRA
                     SSEGAAELVVSKERRVAADCAPGCSELARRTCLEVATLLGDFCEQMCVVGGLAPSLII
                     DQAHRGAGVEEHIGTIELDLDLSIAVLDDQLYKEIAGRLRDADFSPDKNPAGKPTTQR
                     WVSRQGVTVDFLIPPSSSADRGGRLRNLEEGFAAIITPALDLAFQDVPMVELAGTLPS
                     GGAAVRTIQVCGPGAFIVLKALAFDKRGKPKDAYDLYYVPRNHPDSVERIGQRIRGFA
                     DRSEVRDAASVLRWDFARVDAVGPTRVAESLGGPDDTLQADVAGFIRSLLESLT"
     gene            complement(4113..6005)
                     /locus_tag="P5316_14380"
     CDS             complement(4113..6005)
                     /locus_tag="P5316_14380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR86190.1"
                     /translation="MSTNWRDRYAAKLVPAAEAIKRIGRGSRVFVGSACGEPQHLVRT
                     MVEAGAGLADTEVIHVLTLGVAPYTDPRYAPNFRANAFFIGNSVRGPVNECRADYTPI
                     FLSQVPGMFKSRRIDIDAALIMVSTPDEHGNCSLGVSVDITKAAAESAKLVIAQVNKH
                     MPRTLGDCFIHADQIDFLVEYDEPLLEWPVAQSDDVTEEIADHIARLIPDGATLQLGI
                     GRMPDAVLAHLTDKNDLGIHTEMFSDGVLKLIRSGNITGKYKTIHRGKTVGSFAAGSR
                     ELFEFLNNNPQIEMHPSDYTNHPFIISQHDNMVAINAALEIDLTGQVCADSLGQKLYS
                     GMGGHADFMRGAALAKNGKPIIAMPSTARSAEGICSRIVPCLHEGAGVVTTRGDIHYV
                     VTEYGVAYLHGKTMRERAMALINIAHPDFRAELLHAAKRRRLVYPNQIMPPARHEYPD
                     HLEKTYTLRNGQEVLLRPIKPDDEPLMKEMFYSFSEKTVYLRYHGSLKSMPHNKLQVF
                     CNVDYETEMALVAVVGPPGHEEIVGVGRYMTDAAKTSAEFAFVVQDNWQRQGLGTLLF
                     NRLVEIARDAGIRKFGADVLAENSGMLKIFHRSGLVVHTSTEGGVVRVEMAVPGAGKD
                     TRREGT"
     gene            complement(6009..8183)
                     /locus_tag="P5316_14385"
     CDS             complement(6009..8183)
                     /locus_tag="P5316_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRR86191.1"
                     /translation="MPLSTEASPTGQTDQRQTRDLEPLFTPRSIAVIGASRRVGTAGS
                     DIFRNLIYGGYTGVVYPVNPKTKSILGIRCVPNVSAIDETVDLAVIIVPAPGVEAVVT
                     ECADRGTKHFLIISAGFKEIGGDGAEREQRLKALAAERGLSIVGPNCLGIVNTHPDVR
                     MNAAFGGEMPQAGCLGLISQSGALAAALLDYVQGQAIGFSRFVSFGNKCDVTEIDLLR
                     VIAADPSTKVILMYIEDLSGGQAFIDAAHEITHGPNPKPILVIKTGRTQEGAAAAASH
                     TGSLAGSDEVYDAIFSQAGVIRVESVGELFDLAEMFADPPMSRGRQTAIVTNAGGPGI
                     MATDACIRYGLKLAKFQEYTTKSLRFQLPPTSNLKNPVDVIGDARHDRYRAALDAVIA
                     DEGVDQVLVVVTPQSMTNVMEIAEVIGEAKQFTDKPVAACLMGLEDVAGAIDLLQKKY
                     GVPTYRFPENAMKALAAKAKFGEWVRSPVQEYERFEANREGATAIFAEERRAGRNQLV
                     EIKALDALRHYGFPLVPYSLAASAEEAASAAEKIGYPVVMKISGPKILHKTDVGGVRL
                     NLKDADSVRQAYDEILAAVRSKMGREVEIWGVLIQKMLPPGKEIILGMSRDPRFGPLL
                     MFGLGGIYTEALRDVAFRLAPIRKNVASEMVTSIRSHRLLQGVRGEAPSDTTAIAACI
                     MRLSQFVTENPDVKELDINPLIVYARGSGAMVADARIILSES"
     gene            complement(8666..9667)
                     /locus_tag="P5316_14390"
     CDS             complement(8666..9667)
                     /locus_tag="P5316_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HRR86192.1"
                     /translation="MDLATTYLGFKMANPFMPGASPMSDNLDTVRELEDAGASAIVMR
                     SLFEEQLAAEGMTAYQSMDGPAESFAEALTYFPTPDNYVLGPDEYLEQIRKIKQAVGI
                     PVIASLNGSTPGGWLSYARQMAEAGADAIELHVFQVPTDPDEPSGARFAATIDMVKSV
                     KQAVKVPVAVKLSPFYSSFANVARELEKVGADGLVLFNRFYQPDIDVDNLEVIQHLTL
                     STSAELPLRLRWLAILSGRVSPSLAVTGGVHTPLDAIKAVMCGAHAVQIVSALLQNGP
                     QYIRTMIRKVSAWLEEHDYASLRQMQGSMSLQKCPDPQAFIRGNYMKLLQTWRGWNG"
     gene            complement(9742..>13134)
                     /gene="nifJ"
                     /locus_tag="P5316_14395"
     CDS             complement(9742..>13134)
                     /gene="nifJ"
                     /locus_tag="P5316_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRR86193.1"
                     /translation="EAGAAAAVHGALQAGALVTTFTASQGLLLMIPSMYKIAGELTSF
                     CMHVTARTLATHALSIFGDHSDVMACRQTGFALLASGSVQEAHDMACIGTAASLQSRV
                     PFLHFFDGFRTSHEVAKMELLSDDDLRFMVEEQYVKAHRDRALTPDRPLLRGTAQNPD
                     TFFQAREACNRFYDACPEIVQATMDKFAGRVGRKYHLFDYVGAPDAERVIILMGSGAE
                     TAHATAEYLVAKGEKVGVVKVRLYRPFSAKHLLQALPKSVKSIAVLDRTKEPGAIGEP
                     LYLDVVAAIREARARGTSPLANDPRIIGGRYGLSSKDFTPAMVKAVYDELAKSNPIQH
                     FTVGIVDDVTHLSLAVDESFDIEPADVVKAVFFGLGADGTVGANKNSIKIIGEETGNH
                     AQGYFVYDSKKSGAMTISHLRFGPNPIRLPYLIKKASFVACHQFVFLEKYDVLSYAAP
                     GAVFLLNSPYGKDEVWDHLPLEVQQDIIDKKLKFYVIDGYAVAKETGMGSRVNTIMQT
                     CFFAISGVLPREEAIDQIKKAIKKTYGKKGEEVVKRNYAAVDATLAKLHEVSVPPRAT
                     ATQKRLPMVSDAAPDFVKRVTAVMMAGLGDALPVSAFPVDGTYDTATTQWEKRNIALE
                     IPIWDPSLCIQCNKCSIVCPHAAIRPKVFDAALLSKAPADFRSTDYKGKEYKGCKFVV
                     QVAPEDCTGCGLCVSVCPAKDKSNPKHKAINMEPQIAIRARERANYTFFLDLPDVDRT
                     TIERVNVKSSQLFRPLFEYSGACTGCGETPYVKLLTQLFGDRLLIGNATGCSSIYGGN
                     LPTTPYCKDHNGRGPAWNNSLFEDAAEFAFGFRLAIDKHIEQARELLVQLGSQIGDNL
                     TSELLSADQSTEAGIKAQRDRVVALRQKLAGIDSEPARRLNLLADYLVKKSVWGMGGD
                     GWAYDIGYGGLDHVCSMRRDINLLVLDTEVYSNTGGQASKSTPIGASAKFASAGKSVG
                     KKDLGLMAMSYGHVYVASVSMGAKDTHTVQAFLEAESYPGPSLIIAYSHCIAHGYDLV
                     HGLEQQKMAVDSGIWPLYRYDPRRQAAGEPPLVLDSTPSGIPVQEYMRNETRFRMVEK
                     IDPARFQRLSIEAQLAATRRVAVYDHLAKLSVPKGNGDGQKA"
BASE COUNT         2387 a         4195 c         4001 g         2551 t
ORIGIN      
        1 gacgcagtcg atgaagttgc ggtgctcgcc gcctttcatt ccttccgtcc ggggtaccac
       61 ctcgctcggg cggtacaagc ggatctcgtt cgggccaatc ttcgatgcca ggatcttcgg
      121 gtcgccggcc tgcaacgggg cgcgccagcc ctcaaagccg atccaaccgt cggtgccttc
      181 aaaccggata cccggtccct tgctgaccac gtttaaggtc acgccgttgg cgtatcggta
      241 gcggaggtcg aattcctcgg ccgtgttgaa cagggcgtcg cgaggcggaa acttcccggt
      301 gccttcgacc tcgatcgggc cggtattctc ggtgccgttg ccccactggg ccaggtcgat
      361 catgtgggcg ccccagtcgg tcagacggcc gccggaataa gccaggttcc accggaagct
      421 gccgtggcaa cgggccgggc agtaaggtgc ttccggcgcc tgtccctgcc acatttcgta
      481 gttgaagccc ttgggcggcg gctgcacctg gcggtcgttg aagttctcgc cgcgggtctc
      541 gttgcctgca ggcagggaaa cgcgaatgtg tcgtagcttc ccgatccggc cgttgcggac
      601 cagctcgcac atgtgaatgt agctgtcgat cgagcggttc tccgaggcgg tctggaagat
      661 acggccggtc tgcttgatga cctcgcacag cgccttgccc tcggccacgg tcaacgtcag
      721 tggtttctca cagatgacgt ctttaccggc cttggcagcc atgatagccg ggatcacatg
      781 ccagtggtcg ggcgtacaat tggcgatcac gtcaatgtct ttacggttga tcagctcgcg
      841 gaaatcgccg taggtcgcac aagctttgta gttgtcgcgg cccagcgact cagcgtagtg
      901 ctcatcgacg cgggccttgc ctgtcaggca gcgcgcctcg tcaacgtcgc agacggcaat
      961 aacctgcgca tcgctgtagc ggaagatctg caccaggttc cagtcgacgc cgtgcggccc
     1021 gcagccgatc agtccgtaga tgatccggtt gctcggagcc gtcgtcccat ccttgccgag
     1081 ggccgaagca cgcacgaagt aaggcgcggc cagacccgcc gccgcccgcc tgatcaaacc
     1141 tcgacgactg atcgctcgat ctgatgacat ctccagactc ctcacgcgac tggtagggcg
     1201 gcccttccgc caatagaggc gtatcatacc caatctgagg aggatgtcgc tcggcgcgaa
     1261 ccggcccgga tggcgaccgc aaaccgcctc gcctcctccg ccgagagccg agacctgatc
     1321 gaaggatcag ttgcgtttcc cgggaaaact gagagggcgg catacagggc aagcccttct
     1381 ccgacgcttt taagatgtca tttccgtccg cacacaaagc cttggttttg cagcggcctg
     1441 caaaacccaa ttgattctat cgtaaccgat tgtgcacaaa agatttgcga cgatctcaaa
     1501 gagtggctcg aggttgcatt tgtgcggctc cacgagtcgg gcgctgcctt ggccgacggg
     1561 ctgaaacgtc ggagcagatg ccctcacgaa cacgggttgc gatcgggggc gtcgccccgc
     1621 tcagccgccg gggatgagca ggagaacggc acattggata tcagggcttg gtttactcaa
     1681 atacgtgtgt tgaatgcgcc gtcaaccggt tgattttaat gatgatggtc accgagaacg
     1741 acatcctgga acggcttggc gatctcttgg ctgaactggc cggcctgccg gtgaagcctg
     1801 tccggcaggg ggagaaagcc gatcttcggc tgcgcctcgg gtcgcacctg ctggccgtgg
     1861 aggtcaagac caattcccga gcgggcgtgg ttgcacaggc ggccgagaac gctcgccaag
     1921 ccgccgacag cgggaagccg acggcgattc cgttggtggc ggtcccgttc atgggcgagg
     1981 ttggccgcga gatatgccgg gaatgtggtg tgagttacgt ggacatgtcc gggaacgggg
     2041 atatcaacgc acccggtcta cgcattcatg tcgccggtaa gcctaacctg ttcgtgcagc
     2101 agggtcgacc gtcttccgtg ttcgcaccca agagttcgcg gctggcccgc attttgcttg
     2161 ccgagccgaa gcgatggtgg cgtcaggcag aacttgccag cgcgggacaa ctcggggccg
     2221 gttatgtgag ccgaatctgc cgccgcctgg aagaagacca cctgatcgaa cgcgactctg
     2281 agggcagcgt acggccgcgc gatccgaacc tgctgctgga agcatggcag gctcagtatg
     2341 acctgcgtcg ccacgacatc cgccaaggcc atgtcgcggc ccgatcagga gaagaactcg
     2401 cacggcaggt tgccgaggcg tgccggggac tcggcttcga ttacgcgctg actggcctgg
     2461 ccgcggcttg gttgctggct ccgttcgcag gattccgatt ggtcacggtc taccttcgca
     2521 agtcaccgaa cgacaagctt ctccagcggc tcaagtggca cgaggaaaag cagggggcga
     2581 acctctggct gatccggccc aatgacgagg gtgtgttcca cggcgcccag aacgtcaaga
     2641 gcacggtgtg tgtgagcccc gtccaggccc tatctggatc tgcgcaacct gccggaacgg
     2701 gccgaggaag cggcagagca tcttcggaag gagcggctga actggtagtg agcaaagaac
     2761 gacgcgtggc cgccgattgt gcgcccggct gcagcgagct tgcccgccgc acttgcctcg
     2821 aagtggcaac cctgcttggc gacttttgcg aacagatgtg tgtcgtcggc ggcttggcgc
     2881 cgtccttgat catcgatcag gcccaccggg gggcgggcgt cgaagaacac atcgggacaa
     2941 tcgagctgga cctggacctg tccatcgcgg tgttggacga ccagttgtac aaagagattg
     3001 ccggacggct gcgggacgcg gacttcagcc cggataagaa cccggccggc aagccgacca
     3061 cccaacgctg ggtatccagg caaggcgtca ccgtcgattt cctgattccg ccatcgtcca
     3121 gtgcagaccg cggaggacgc ctgcgcaacc tggaggaagg cttcgccgca atcatcaccc
     3181 ccgcattgga cctggccttt caagatgtgc cgatggtcga gcttgccggc acgttgccga
     3241 gcgggggcgc ggcggtgcga acgattcaag tttgcggacc gggcgcgttc atcgtgctga
     3301 aggccctggc gttcgacaag cgcggcaaac ccaaagatgc ctacgacctt tactacgtac
     3361 cgcgcaatca cccggacagt gtcgaacgta tcggtcaacg gattcgcggc ttcgcagatc
     3421 gatccgaggt ccgcgacgcg gcgtcggtgc tccggtggga ctttgcacgt gttgatgccg
     3481 tgggacccac tcgcgtagca gagtctctgg gcggtccgga tgataccctg caagccgatg
     3541 tcgcgggctt catccgcagc ctgctcgaaa gcttgacttg agggctggca agtgcccggt
     3601 ccgccggcaa cgaaaggcga agtcattgca cttccttgca ttggcccgcg aatgcaaacc
     3661 gagccatgaa acagagagca aatggagaca gaatgcgctt tcgggacccg cgttttcccg
     3721 tgcgtgaagg ccggcgttat gccggatgac tgccggtggc agggtgcaac gcgcaccgcg
     3781 tcatgcactt ggggggcttc ggagcagatt gtgccggcag gttcaagcct cctggtctca
     3841 tcggaaacca agatggggcc gtgtgatggt ttggaggggt ggcggtcagt gcgtacttga
     3901 gtcagaggtt ggtaccgggg caggcgaatt cggcggcgtc gaggtgcgcc ggcaaatact
     3961 gaacggcccg gtttgagcac gggttggccg gagcgcgcaa tcgcagaggt caatggaaac
     4021 cgtcgatccc ctcaaggagg aatccgacca acggccgaga tcagggggcc tgaagccgct
     4081 tcgggacgcg gaagagcgcc gcgggtcggg gcctatgtcc cttcccttcg agtgtccttc
     4141 ccggcccccg gcacggccat ttccacgcgg actacgccac cttcggttga ggtatgcacc
     4201 accagccccg agcggtggaa gatcttgagc atgccgctgt tctcggccag cacgtcggct
     4261 ccgaacttgc ggatgcccgc gtcgcgagcg atctccacca atcgattaaa caagagcgtc
     4321 ccaagcccct gtcgctgcca gttgtcctga acgacaaaag cgaattcggc gctggtcttg
     4381 gccgcgtccg tcatgtagcg gccgacaccg acgatctctt cgtgtccggg cggaccgacg
     4441 accgccacaa gggccatttc ggtctcatag tctacgttgc agaaaacctg cagcttgttg
     4501 tgcggcatcg acttgagcga accgtgataa cgaagataaa cggtcttctc gctgaacgaa
     4561 tagaacatct ccttcatcag cggttcgtcg tcgggcttga tcgggcgaag cagaacctcc
     4621 tgcccattgc gaagcgtgta cgtcttttcc agatggtccg ggtactcgtg ccgggcaggc
     4681 ggcatgatct ggttcggata gaccagccgg cgacgtttcg ccgcgtgcag cagctcggcc
     4741 cgaaagtccg ggtgcgcgat gttgatcagg gccatcgcac gctcccgcat ggtcttgccg
     4801 tgcagatacg ccacgccgta ttccgtgacc acgtagtgaa tatcgccgcg agtggtcacc
     4861 acgcctgcgc cttcgtgcag gcacggaacg attcgtgaac agataccctc cgccgatcgg
     4921 gccgtgctcg gcatcgcgat gatcggcttg ccgttcttgg ccagggccgc gccccgcatg
     4981 aagtccgcgt ggccgcccat gccgctgtag agcttctgtc cgagtgagtc ggcacatacc
     5041 tgtccggtca gatcgatttc cagcgccgca ttgatggcca ccatgttgtc gtgctgggag
     5101 atgatgaacg gatggttggt gtagtccgac gggtgcatct cgatctgcgg gttgttgttc
     5161 agaaactcga acaactcgcg gctgcccgcg gcgaagctgc ccaccgtttt gccccgatgg
     5221 atcgtcttgt acttgcccgt gatgttgccg ctccggataa gcttcagtac cccgtccgag
     5281 aacatctccg tgtgaatgcc gagatcgttc ttgtccgtca ggtgagccag cacggcatcc
     5341 ggcatgcgcc cgatgcccag ttgcaacgtc gcaccgtccg gaatcagccg ggcgatgtgg
     5401 tcggcgatct cttccgtcac gtcatcggat tgagcgaccg gccactccag caacggttca
     5461 tcgtattcca ccaggaagtc gatctggtcg gcgtggataa agcagtcgcc gagggttcgg
     5521 ggcatgtgct tgttcacctg agcgatgacc agcttggccg attcggcggc cgccttggtg
     5581 atgtccaccg agacgcccag gctgcagttg ccgtgctcgt cgggcgtgct gaccatgatc
     5641 agcgccgcgt cgatgtcaat gcgccgggac ttgaacatcc ccggaacctg ggacaggaaa
     5701 atcggcgtgt aatcggcacg gcattcattc accggcccgc gcacgctgtt gccgatgaag
     5761 aacgcgttgg cccggaaatt cggcgcatag cgcgggtccg tataaggggc cactccgagg
     5821 gtcagcacgt ggatcacctc cgtgtcggcc aatccggcgc ctgcttccac catcgttcgc
     5881 acgagatgct gcggttcgcc gcaggccgaa cccacgaaca cccgactgcc ccggccgatc
     5941 cgcttgatgg cctcggcggc gggtaccagc tttgcggcat atcgatcacg ccagttcgta
     6001 ctcatcgctc atgactccga caaaatgatc cgcgcgtcgg cgaccatcgc gccgctgccg
     6061 cgggcgtaga cgatcaaggg gttgatatcc aattccttca cgtccgggtt ctcggtcacg
     6121 aattgtgaca accgcatgat gcacgccgca atcgccgtcg tgtccgacgg ggcttctccc
     6181 cgcacgccct gcagaagacg atgggaacga atgctggtca ccatttcgct tgccacgttc
     6241 ttgcggatcg gggccagacg gaaggccaca tcccgcaggg cctcggtgta gatccctccc
     6301 agcccgaaca tcagcaacgg cccgaaacgc gggtcgcggc tcatgcccag aatgatctcc
     6361 ttgcccggcg gcagcatctt ctgaatcagc actccccaga tctccacctc acgccccatc
     6421 ttgctccgca ccgccgccag tatctcgtcg tacgcttggc ggaccgagtc ggcgtctttg
     6481 aggttcaacc tcacgccgcc cacgtcggtc ttgtgcagaa tcttggggcc ggagatcttc
     6541 attaccaccg ggtagccgat cttctcggcg gccgatgccg cctcctccgc cgaggcggcc
     6601 agagaatagg gcaccagcgg gaaaccgtag tgccgcaacg catccaacgc cttgatttcc
     6661 accagttggt ttcgaccggc acgccgctcc tcggcaaaga tcgcggttgc cccctcgcgg
     6721 ttcgcctcaa atcgttcata ctcctgcacc ggcgaccgga cccattcacc aaatttggcc
     6781 ttggcggcca gcgccttcat cgcgttctcc ggaaagcggt aggtgggtac gccgtatttc
     6841 ttctgaagca aatcaatcgc gccggcgacg tcttcaagac ccatcaggca cgctgccacg
     6901 ggcttgtccg tgaactgctt tgcttctccg atgacctccg cgatctccat gacgttggtc
     6961 atcgactgcg gcgtgaccac caccagaacc tggtccaccc cttcgtccgc gataaccgca
     7021 tccagcgccg cgcggtaacg gtcatgtcgg gcgtccccga tgacgtcaac cgggttcttc
     7081 aggttgctcg tcggcggaag ctggaatcga agcgacttgg tcgtgtactc ctgaaacttg
     7141 gcaagcttca atccgtagcg aatacaggcg tcggtggcca tgatgcccgg tccgcccgcg
     7201 ttggtgacaa tcgccgtctg ccgaccccgg gacatcggcg ggtccgcgaa catctccgcc
     7261 aggtcaaaca actctcccac ggactcaacg cgaatgacgc cggcctgtga gaagatcgcg
     7321 tcataaacct cgtcagaacc ggccagcgaa cctgtgtgag aggcggccgc cgcggcacct
     7381 tcctgcgtcc gtcccgtttt gatcaccaga atcggcttgg ggttcggccc gtgcgtgatc
     7441 tcgtgcgccg cgtcaatgaa ggcctggccg cccgacaggt cctcgatgta catgaggatg
     7501 accttcgtgc tcgggtcggc ggcgatcacc ctgagcagat cgatctcggt gacgtcgcac
     7561 ttgttgccga agctgacaaa tcgcgagaaa ccgattgcct gtccctgcac gtagtcgagc
     7621 agcgccgcag ccaacgcccc gctctgggaa atcaggccca gacacccggc ctgcggcatc
     7681 tcgccgccaa acgccgcgtt catccggaca tccgggtgcg tgttgacgat gcccagacag
     7741 ttggggccta cgatcgagag cccgcgctcc gcggccaggg cctttaacct ctgctctcgc
     7801 tccgcgccat cgccgccgat ctccttgaac cccgccgaga tgatgagaaa gtgtttcgtt
     7861 cccctgtccg cgcattcggt caccaccgcc tcgactcccg gcgcgggaac gatgatgacc
     7921 gccaggtcga cggtttcatc gatggccgaa acgttgggaa cacaacgaat acccaggatg
     7981 ctcttggtct tggggttgac cgggtaaacc acgccggtat agccgccata gatcagattg
     8041 cggaaaatgt cgctcccggc cgtgcctacg cgacgcgaag cgccgatcac ggcgattgat
     8101 cgtggggtga acaacggctc cagatcgcga gtctgtcgtt ggtcggtttg gccggttggc
     8161 gatgcttcgg tgctcagagg catctggctc gactcctgct ggaaaaactg cggttcccgc
     8221 catctcgtga ccgcccggcg ggtttccgct gacagtcgga tggtcagcat accatccgaa
     8281 tctgcagttt accatggggt tgctcgcacc ggcggcgagg cgaagtcgcc gtccatgcgg
     8341 ggtgttacgg cagctcggcc cgggctgcgc ttcccaggtc gttgcggtgg gctggccggc
     8401 atctcccatg ccttccgcac aaaggataac cgcagcgtct gcacgtcgca agaaacatca
     8461 ctgccgccgc cgtccacgcc ccgttgagcg tccttgttcg ttttccggtc cccgggcgat
     8521 ctccggaagc aacttgtcgg catgaatgct cactcggctg cctccgatga gcgacctcgg
     8581 cgatcggctg agttcaaaaa gacgacgacg gccggctcca acgccggccg ccaccggttc
     8641 atgtctcaat cgcgccccga tgccctcagc cgttccagcc ccgccaggtc tgcagcagct
     8701 tcatgtagtt gccccgtatg aatgcctgtg ggtcggggca cttctgcaag ctcatgctcc
     8761 cttgcatctg acgcagtgaa gcgtagtcgt gctcttccag ccacgcgctg accttgcgga
     8821 tcatggtccg gatgtattgc ggcccgtttt ggagcaaagc cgaaacaatc tgcacggcgt
     8881 gagccccgca catgaccgcc ttgatggcgt ccagcggcgt gtgaacgccc cccgtcaccg
     8941 ccagcgaagg cgacacccga ccggagagga tcgccagcca ccgcaaccgc aggggcagtt
     9001 ccgccgaagt ggacaacgtc aaatgctgaa tcacctcaag attgtcaaca tcgatgtccg
     9061 gctggtagaa acggttgaaa agaaccagcc cgtcagcccc gaccttctcc agctcccgcg
     9121 cgacgttcgc gaaggacgaa tagaaggggg acagcttgac cgccaccggc accttcacgg
     9181 cttgcttgac cgacttgacc atgtcgatgg tcgcggcgaa acgggctccg ctcggctcat
     9241 ccggatcggt cggaacctgg aagacgtgta actcgatggc gtccgccccg gcttccgcca
     9301 tctgccgcgc gtagctcagc cagcctcccg gcgtgctccc gttcagcgag gcgatcaccg
     9361 gaatgcccac cgcctgcttg atcttgcgga tctgttccag gtactcgtcc ggtccaagta
     9421 cgtagttgtc aggagtgggg aaataggtca gggcttccgc gaaggattcg gccggcccat
     9481 ccatgctctg ataggccgtc atgccctccg ctgccagttg ttcctcgaac agcgatctca
     9541 tgacgatcgc cgaagcaccg gcgtcttcaa gttcgcggac cgtgtccaga ttgtccgaca
     9601 tgggcgaagc gcccggcata aacggattcg ccatcttaaa accgaggtag gtcgtcgcaa
     9661 gatccatgac tcatgtctcc agtctgtcag atgagggtga actcgtagct cgccggtctc
     9721 tgccgtttct cgcacctggt cctacgcctt ctgaccgtct ccgttgccct tgggcacgct
     9781 gagctttgcc agatgatcgt acaccgccac gcgtctcgtg gcggcaagct gcgcctcgat
     9841 cgacagccgt tggaaccggg cgggatcgat cttctcaacc atccggaacc gggtctcatt
     9901 acgcatgtat tcctgcaccg gaatcccgct gggcgtcgag tcaagcacga gcggcggctc
     9961 tccggcggct tgtcgccgcg gatcgtaccg gtagagcggc cagatgccgc tgtccacggc
    10021 catcttctgt tgctccaagc catggacgag gtcgtagccg tgggcaatac agtgcgagta
    10081 ggcgatgatc agcgaggggc cgggatagga ttccgcttcc aggaacgctt ggacggtgtg
    10141 ggtgtccttc gcgcccatgg acacgctggc tacgtacaca tgtccgtagc tcatggccat
    10201 caggcccaga tccttcttgc ctaccgactt gcctgctgag gcgaacttgg ccgatgcccc
    10261 gatcggcgtg gatttcgacg cctgaccgcc cgtgttcgag taaacctcgg tgtccagtac
    10321 cagcaggttg atgtcccgcc tcatgctgca aacgtggtcc aggccgccgt agccgatgtc
    10381 gtaggcccaa ccgtcgccgc ccattcccca gacgctcttt ttcaccaggt agtcggccag
    10441 aaggttcagc cgtcgagccg gctcgctgtc gatcccggcc agtttctgac gcagtgcgac
    10501 cacccggtcg cgctgggcct tgatccccgc ttcggtggac tggtcggcgc tcaacagctc
    10561 gctggtcagg ttgtcgccga tctgcgaacc gagttggacc agcagttcgc gggcctgctc
    10621 aatatgcttg tcgatcgcca gtctgaagcc gaaggcgaat tcggccgcgt cctcaaacag
    10681 cgagttgttc catgcagggc cgcgcccgtt gtggtctttg cagtagggcg tcgtcggcag
    10741 attgccgccg tagatcgatg aacagcccgt cgcgttcccg atcagcaggc ggtcgccgaa
    10801 caactgggtc agcagcttga cgtaaggagt ctcgccgcac ccggtacaag cccccgaata
    10861 ctcgaacagc ggtcggaaaa gctgcgagct cttgacgttg acccgctcga tggtcgtccg
    10921 gtccacgtcg ggcaggtcga ggaagaacgt atagttggcg cgctcgcggg ctcggatcgc
    10981 aatctgcggc tccatattga tggctttgtg cttcggattg gacttgtcct tggccgggca
    11041 cacgctgacg caaagcccgc agcccgtgca atcctccgga gccacctgga ccacaaactt
    11101 gcagcccttg tactccttgc ccttgtagtc cgtcgagcgg aaatccgccg gcgccttcga
    11161 aagcaacgcg gcgtcgaaca ccttcggtcg gatggccgca tgcgggcaga cgatcgagca
    11221 cttgttgcac tggatgcaga gcgatgggtc ccagatcggg atttcaaggg cgatgttccg
    11281 cttctcccac tgagtcgtgg ccgtgtcgta ggtgccgtcg accgggaagg cgctcaccgg
    11341 cagggcgtcg cccaggccgg ccatcattac cgcagtcacg cgcttgacga agtccggagc
    11401 ggcatccgag accatcggca gccgcttctg cgtggccgtc gcccgtggcg gaacgctcac
    11461 ttcgtgcagc ttggcaagcg tggcgtccac cgcggcgtag ttgcgcttga ccacctcttc
    11521 gcctttcttg ccgtacgtct tcttgatcgc tttcttgatc tggtcgatgg cctcctcacg
    11581 cggcagaacg cccgaaatgg cgaagaagca ggtctgcatg atggtgttta cccgcgaccc
    11641 catcccggtc tccttggcca ccgcgtaacc gtcaatgacg tagaacttca gcttcttgtc
    11701 gatgatgtcc tgctgcacct ccagcggcag atgatcccag acctcatcct tgccgtaagg
    11761 cgagttcaac aggaataccg cccccggagc cgcgtagctc agcacgtcgt atttctcgag
    11821 gaacacgaac tgatggcagg ccacgaagct cgccttcttg atcaggtaag gcagacggat
    11881 cggattcggc ccgaagcgaa gatgcgagat cgtcatcgcg cccgacttct tcgagtcgta
    11941 gacgaagtag ccctgagcgt ggttgcccgt ctcctcgccg atgatcttga tcgagttctt
    12001 gttcgccccc acggtgccat cggcgccgag accgaagaac accgctttga cgacgtccgc
    12061 cggctcgatg tcaaagctct cgtctacggc cagcgacaga tgcgtcacgt cgtccacgat
    12121 gcccaccgtg aaatgctgta tcgggttgct cttggccagc tcatcgtaca ccgccttgac
    12181 cattgccggc gtgaagtcct tgctcgacaa cccgtatcga ccgccgatga tccgcgggtc
    12241 gttggccagc ggcgacgtgc cccgtgcccg cgcctcccgg atggccgcta ccacgtcaag
    12301 gtacaacggc tctccgatcg caccaggctc cttggtccgg tccagaacgg cgatcgattt
    12361 cacgctcttg ggcaacgctt gcagcagatg cttggccgag aaaggacgat agaggcgaac
    12421 tttcaccacc ccgaccttct cccccttggc gaccaggtat tcggcggtcg cgtgggcggt
    12481 ttccgccccc gagcccatca gaatgatcac ccgttccgca tccggcgcgc cgacataatc
    12541 aaacaggtgg tacttgcggc cgacccggcc ggcaaacttg tccattgtcg cctggacgat
    12601 ctccgggcac gcgtcataga agcggttgca ggcctcgcga gcctggaaga aggtgtccgg
    12661 gttctgggcc gtgccccgaa gcaaaggtcg gtccggcgtc agcgcccgat cgcggtgcgc
    12721 cttgacgtac tgctcctcaa ccatgaaacg caaatcgtcg tccgacagca actccatctt
    12781 ggccacttca tgcgaggtcc ggaatccgtc gaagaagtgc aggaatggta cgcggctctg
    12841 aagcgaggct gccgtgccga tgcacgccat gtcgtgggct tcctgcaccg agcccgaggc
    12901 cagcagagca aaacccgtct gacgacaggc catgacgtcg gagtgatcac cgaaaatcga
    12961 caaggcgtgt gtcgcaagag tgcgggccgt cacgtgcatg cagaacgagg tcagctcgcc
    13021 ggcaattttg tacatgctcg ggatcatcag cagcagaccc tgactcgccg taaacgtcgt
    13081 caccaacgca ccggcctgca gggccccgtg gaccgccgcc gccgcaccgg cctc
//
LOCUS       DAOTPW010000161        13061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4604_length_13061_cov_5.556897, whole genome shotgun sequence.
ACCESSION   DAOTPW010000161 DAOTPW010000000
VERSION     DAOTPW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13061
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4604_length_13061_cov_5.556897"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1535)
                     /locus_tag="P5316_14400"
     CDS             complement(144..1535)
                     /locus_tag="P5316_14400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRR86194.1"
                     /translation="MARCSLTAWTLDSREEPVIRVGMVLPEDGMDSILLKIPSRGYLV
                     SGGDGPGQMFRSVMAKVTVENRQVSFLSEGGVAGPADVIKVSPEEAVPQTRGAGVQLR
                     GVVAGRGFHWEKRIHPTVGGMLEFRAIRGRLLVVNELPLEEYLPGVIAGEMSGDCPME
                     FLKSQTVVARAWVLAHTEKKHTEYPVDRCNDDCCQRYHGTTDLTERAVEAVRQTRGQV
                     LVDRKRTLIDANYSKSCGGIIESPQHVWFVQKAGQRATVDAPKDSAAHRFFPLSEKLL
                     KEYLTGEWLKQTDVFCSPNVVPEKSLPSYLSKIDEGGGHFRWRVDYDRHELEKILREK
                     FFSRQDPSKVAFLDTLLGLRVCQRGDSGRAKDLEIEYLDPMGVTHKVHVISEYSIREV
                     LHEKFLFSSAFVIEIQRDTDGIPRKISLIGGGWGHGAGMCQIGALGMALRGYKYEAIL
                     KHYFEDVQLHDCY"
     gene            complement(1778..3259)
                     /locus_tag="P5316_14405"
     CDS             complement(1778..3259)
                     /locus_tag="P5316_14405"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRR86195.1"
                     /translation="MSLLKAASWVFIVAGAVCARADTQWSEVAPGISYREYALDGPVR
                     VFVARADRSKKNWTIDSMTSLGTVKGGRETVPDMAVRYDDTVTFDGRRYDVKVAINGD
                     YFDMRTGVAAGGQVISGWFVKRFGEYSGNSGFVWTLDGKGFLGGNVRNDASLQRVVFA
                     DRSDMRIDKLNEPRGTDELALYTPHYADNTGTGDDGVEVLVQVSAPIAIMPPSPGIKC
                     QVAQVRENSGSTPLPFDHVVLSANGRAARELRKQAKAGDSLHIDLRLREFGNEDIGLA
                     PCNWTNAYASVGGPKCVLVNGKVPRDWEAKAARYAAEGRKHGSVIKDPRTAVAFDDRY
                     VYFLVVDGRSPESIGMTFTEVGIFCKDELKATNAILQDGGGSSTLWVDGKVKNNPSGK
                     AGTDAAGVLRPVANGYFMALVLRPELSESLRVGERAVFKNAGLLRLGPGTTYGQAAAV
                     RAGQEVRILPHALNGILAKGNHWWFCGLEGGRGWASEDQLRGR"
     gene            3500..4237
                     /gene="ispD"
                     /locus_tag="P5316_14410"
     CDS             3500..4237
                     /gene="ispD"
                     /locus_tag="P5316_14410"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRR86196.1"
                     /translation="MSKVAVIIVGAGKGTRFGGNEKKIFAKVDGQPLFLRSIQLFVNR
                     DDVCQTILVAAAEDEEQIREKYGANLGFMGVKMVTGGPTRPDSVANGLAVVKEDAQFV
                     AVHDAARPCTTDEMIDAVFAEAAKTGAALLAAPLRGTIKRVSESKVIDETVSRQGLWE
                     AQTPQVFRRNLIMAAYEKRAEFRGELTDDAQLVEVMGHPVAVVECDFTNLKVTTRGDL
                     TLANSIVKFRPKPKPKGPLGAFEEAQW"
     gene            4383..5243
                     /locus_tag="P5316_14415"
     CDS             4383..5243
                     /locus_tag="P5316_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86197.1"
                     /translation="MRKHSTMLLFQLGAVVSLLWVLTLPAGCKTDSLASEKIERPPGP
                     MTSLENPAGANLPEISIAGRDELDIVEELVMHRTQYARLLRALVIYYNEHGMSDKAMW
                     ARSELADLQRVKPYDYIAEAATPVETLRPKDSIPEANKLFEEGMALLKEGGHGTPIFY
                     NQEIMKKALAKFKELVEKYPTSDKIAQAAFYIGEIHKEYFEEKDNDIALLWYKRAIDW
                     DPNIQLPARFQMAAVWDYRLHNRDKALYWYQEAIEKEQFNRSNVLWAKSRIRELTPAR
                     VETQPTEEER"
     gene            5539..6375
                     /locus_tag="P5316_14420"
     CDS             5539..6375
                     /locus_tag="P5316_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR86198.1"
                     /translation="MKLATCSEPWRDVPIEEVFGIAARIGFEGVELAPFTIAEHVDQI
                     SSDRRKTIARAAADAGVSVVGLHWLFVSPKGLHITTPDDAVRRKSADYLRSLVHFCGD
                     VGGKVMTLGSPNQRSIVPPTTFDEGWKRAKDVLASVAHTCAERGVTICFEALSPKETN
                     FINTAEEAARLADEIGHPNIDIMLDMKAMASMPDGIEGTIRRFGKRARHFHANHPSGK
                     GVGMPLASGDGEPLDLKSILKTLAETGFDRWVSVEPFDYKPDPTTVGEHAYRTLKAAL
                     PA"
     gene            6413..7684
                     /locus_tag="P5316_14425"
     CDS             6413..7684
                     /locus_tag="P5316_14425"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRR86199.1"
                     /translation="MVNKMMGIAWIAAIVAGMGLTASTARADKVTITVGDKDIPAGPM
                     WFKPAKPLDPQQYYYLSGKDADLLVQIDKEDRVWWWQNKPLKAGQTYTWELLVAGTGV
                     NPEPGGLPARVRKAGDEQVDIKISGKPFTTLVFKKSDPKVYLYPVIGPTGEPMTRDFP
                     MKDNPAEKEAKRQDHPHHRSLWCAHGDIRTEDMTKPGSNYWNEVKDAPIEKVPHQVLT
                     KIVGQASGPVFGQVIAEIDWINEKGEKDFSEQRSYMFLAGEDLRIIDVRSVFKFDQKD
                     VTFGDTKEGGIVSVRTAITIDEKGGGKRYNSAGKSGEDTWGQPAEWCDYVGKVNDKVV
                     GIAIMDHPKNYGHPTHWHIRDYGLYTANPFGLKDFTKDAAKDGSHTWKKGESVEFNYR
                     ILLHQGDTQAAGIADQWRLYSDPPKFTVTEK"
     gene            complement(7720..8115)
                     /locus_tag="P5316_14430"
     CDS             complement(7720..8115)
                     /locus_tag="P5316_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRR86200.1"
                     /translation="MAKDYYVVLGLTVDATPAQIKQAYRRKALELHPDHYGPDVEPFL
                     EVQEAYAVLSDPRRRDAYDRSVQQARRIKVRSAAAPDIIGPRRPPAEPLVPQQAPLDW
                     GDVPIPESSWAFFRSFDEIFSRLWFDFTE"
     gene            complement(8168..8659)
                     /locus_tag="P5316_14435"
     CDS             complement(8168..8659)
                     /locus_tag="P5316_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR86201.1"
                     /translation="MNLIPWRNKREARNGSRTEAPPLARLRSEMDQLFERFWRDPWST
                     SLADLMPAGVGVGLRLNLAESENDVTLTAELPGVDPKDVEISVSDNMLTISGEKKQEK
                     EEKKRNYHYVERSFGSFRRSIQLPSSVDPNKVDATYRNGVLTVTLQKRPDAKPKRIAV
                     KSG"
     gene            complement(8674..9075)
                     /locus_tag="P5316_14440"
     CDS             complement(8674..9075)
                     /locus_tag="P5316_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR86202.1"
                     /translation="MSTALTVKKEQDKLAQVERARDRRTYMPNVDIIETSDEFLVLAD
                     MPGVAPDAIDIQYEQGVLTINGRVDPRQDEDKTNYLLHEYGVGDFYRSFRLGEGIETD
                     KIEARLKDGVMELHLPKSEACKPRKITVKAS"
     gene            complement(9128..9568)
                     /locus_tag="P5316_14445"
     CDS             complement(9128..9568)
                     /locus_tag="P5316_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR86203.1"
                     /translation="MFAGTMNPFAVMNELRREVERIFEGYGFPFNGGWTSRLTAFPAL
                     NIWEDGEALYAEAEVPGVSENDIEIYAVGNELTIKGTRKPRESEGASYHRRERGTGEF
                     TRVVTLPAEVNAEKVQATLKDGVLTVTLPKVDEAKPRKIAVKTS"
     gene            complement(10133..11338)
                     /locus_tag="P5316_14450"
     CDS             complement(10133..11338)
                     /locus_tag="P5316_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86204.1"
                     /translation="MNPKHVFIGPAVLSVALLAVPPAWADPPGWLHTFDDNTFEGLVP
                     WDYISWQTDGAIVTFFPTTATPCPSCSPALRLSFTSSIDRAVGLFSEYGTEFSNANGG
                     VSAKLLIKTSTNPHMFATPDKNTFFVALAVNMDPQFSSDKTGAHFKGYWVYVGDAGAN
                     PNANPPTNNRLEMAIQYLDPSGPHDLCSPTPGSIRFDPFDPTKDWWMRFEALDDGAGA
                     VLVRGRIWPDDGTPEPSTWDVYCVDTVYKYTSGIVGFGAEQRLIGPAPYTSYIDLDNV
                     SASLVPEYNCYNLIDDDLDSLTDCADPDCAASAACACNDPFADADGDGDVDQADFAVF
                     QACFSGAGGGLFADCECFDRDDMNADQRFNPPDDGDGDVDNTDLDKFEACASGPGIAA
                     DQTCDNANY"
     gene            12032..12733
                     /locus_tag="P5316_14455"
     CDS             12032..12733
                     /locus_tag="P5316_14455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86205.1"
                     /translation="MLRLFAVVDLVAVLVVPAVGAYQYDFTLDQSRSNITITLPIFAG
                     GATATSPLSGTYSMMLDSPSGTYNTRDWNVQASLKTISATNTAQMILISVPYNIYVQV
                     DPGDFRAMDWNQNKGAIPSTTLAGGPNISSGDLSTDILLSIYNEVTHSYVTRNGAAGE
                     WFMMNWTLQVSDDDWLGVAGTGDVESCVTATYYDQNLIKYVTKLYGRGTLIPEPATLA
                     LLGLGGLALLCRRPV"
BASE COUNT         2701 a         4110 c         3735 g         2515 t
ORIGIN      
        1 gcgtgggcat gcgcctccaa atgaatcccg aagcaggtag ggcaggtcag agccccgcca
       61 agtcgccccg acgaagcacg gcgccgacct gccgccaccc taaagctttt gctggtaatg
      121 acttaaagag aatcaagccc gggtcaatag cagtcgtgga gttgcacgtc ctcaaaataa
      181 tgcttgagga tggcctcgta cttgtagcct cgcagagcca tgcccagcgc gccgatctgg
      241 cacatgccgg ccccatgtcc ccagccgccg ccaatcagtg agatctttcg gggtatgccg
      301 tccgtatccc gttgtatctc gatcacaaac gcgctgctga acaggaattt ctcgtgcagc
      361 acctcgcgga tgctgtactc gctgatcaca tggaccttat gcgtcacgcc catcgggtcg
      421 agatactcga tctccaggtc ctttgcccgg ccggagtcgc cccgctggca cacccgcaga
      481 cccagcagag tatccagaaa cgccaccttg ctcggatcct gcctgctgaa gaacttctcc
      541 cgaaggatct tctccagctc gtgccggtcg tagtccaccc gccatcggaa atgaccgccg
      601 ccctcgtcga tcttgctcag gtaagagggc aggctcttct cgggcacgac gttcggactg
      661 cagaatacgt ctgtctgctt gagccattca ccagtcaaat attccttcaa cagtttttca
      721 gagagaggaa agaaccgatg agccgccgag tccttcgggg catcgaccgt ggcccgctgt
      781 ccggccttct gcacgaacca gacgtgctga ggcgattcga tgatccctcc gcagctcttg
      841 gaatagttcg cgtcgatcag cgtcctcttc cggtcgacca acacctgtcc gcgagtctgc
      901 cgcacggcct caaccgcgcg ctcggtcaga tcggtcgtgc cgtgataacg ctggcaacag
      961 tcatcattac accgatcaac aggatactcc gtgtgcttct tctcggtatg agccagaacc
     1021 caggccctgg ccacgaccgt ctgactcttc aggaactcca tcgggcagtc gccgctcatc
     1081 tcgccggcaa tcacgcccgg caggtattcc tccagcggca gttcgttgac caccagcaga
     1141 cgcccgcgga tcgccctgaa ctccagcatt cccccgaccg tcgggtgaat cctcttttcc
     1201 cagtgaaaac ctctgcccgc caccaccccg cgaagctgaa cgcccgcgcc ccgtgtctgc
     1261 ggtaccgcct cctcgggcga caccttgatg acatccgccg ggcccgccac gcccccctcc
     1321 gacaggaacg aaacctgccg gttttcgacc gtgaccttgg ccatgaccga ccggaacatc
     1381 tgcccggggc cgtcgccgcc cgagaccaga tagccccgac tcggaatctt cagcagaatc
     1441 gagtccatgc cgtcctcagg caacaccatc ccgacgcgaa tgacgggttc ctcgcgtgaa
     1501 tcgagcgtcc aggcggtcag actgcaacga gccatcgagt accgttccct ttcaagatca
     1561 aagcgtgtgg ccaaggccgg cactcgctcc ctcaccgccg aaaagccggg agttcaaccg
     1621 agcggccggc atgacacatc accttaatcc gaagatgccg ggcgggcaag ccgctgctcc
     1681 cgccggaatg actgttcttc aggtcgcctt gcctgtaatc ctgcccccgt ccggatgcct
     1741 gccgcccctc tcatgggcgc gagcaccacc gcaaaggcta ccgaccccgc agttgatcct
     1801 cagatgccca gccccggccg ccctcaagac cgcagaacca ccaatgatta cccttcgcga
     1861 gaatcccatt cagagcgtga ggcagaatcc tgacttcctg tcccgcgcga acggcggccg
     1921 cttggccgta ggttgtcccc gggcccagac gcaatagccc agcgttcttg aacaccgctc
     1981 gttccccaac ccgtagactc tccgataact cgggccgcag gacaagggcc ataaagtagc
     2041 cgttggctac gggcctcaga acgccggccg cgtccgtccc cgctttgccg gacgggttgt
     2101 tcttcacctt gccgtctacc cacagcgtgg aagaccctcc cccatcctgg agaatcgcat
     2161 tcgtcgcctt gagctcgtcc ttgcagaaga tgcccacctc ggtgaaggtc atgccgatgc
     2221 tctccgggga acgaccgtcg acgacgagga agtagacgta tctgtcatcg aaggcgacgg
     2281 ccgtgcgcgg atccttgatg actgagccgt gctttctgcc ctcggccgca taccgggcgg
     2341 ccttggcctc ccaatcacgc ggtactttgc cgttgaccag cacgcacttc gggccaccga
     2401 cgctcgcata ggcattggtc cagttgcacg gtgccaggcc gatgtcctcg ttaccgaatt
     2461 ctcgaagccg caaatcgata tggagactgt cgccggcctt tgcctgcttc cgcagctctc
     2521 tggcggcccg gccgttggcg gagagaacca cgtgatcgaa cggcagcggg gttgaaccgc
     2581 tgttctcgcg gacctgggca acttggcact tgatgcccgg agatggaggc attatggcga
     2641 tgggagccga aacctggacg agaacctcga cgccgtcatc gccggtgcct gtgttgtcgg
     2701 cgtaatgcgg ggtgtaaaga gcaagttcgt cggtgccgcg gggttcgttg agcttgtcaa
     2761 tcctcatatc ggacctgtcg gcgaagacca cccgctgtag cgaagcatcg ttgcggacgt
     2821 tgcccccgag aaagcctttg ccatcaagcg tccagacaaa tccgctgttg cccgagtatt
     2881 cgccgaaacg tttgacgaac catccggaga tgacctgccc gccggccgca acgcccgtcc
     2941 gcatgtcaaa gtaatcaccg ttgatggcca cttttacgtc gtagcgacga ccgtcgaagg
     3001 tgaccgtatc gtcgtaccgc accgccatat cgggtaccgt ctcgcgccca cccttgactg
     3061 tcccaaggct ggtcatcgag tcgatggtcc agttcttctt cgaccggtcg gcccgggcaa
     3121 cgaacacccg aacaggaccg tcgagcgcgt actcacggta actgataccc ggggcgactt
     3181 ccgaccactg ggtatcggct ctcgcgcaga ccgcgccggc aacaataaag acccagcttg
     3241 ccgccttcag cagactcatg gtcgccaacc tccggacact cgacggatca gattcggcaa
     3301 tggaagatca tagcagccgg accacgagat gacaccgggc aatgacgaaa acagaacgag
     3361 actccacgac tctgcagtgg cgacaccgcc aggccggccg acgacgcacg tcggcttgcc
     3421 gagatcgatg gcgatctata gattctatct tgtggaagcg agcgctcgtg cggcccgcgg
     3481 caacttggga ggctctacga tgtctaaggt cgcagtcatc atcgtcgggg cgggaaaggg
     3541 tacgcgattc ggcggtaacg agaagaagat cttcgccaag gtcgacggcc agcccttatt
     3601 cctccgttcg atccagttgt ttgtcaaccg cgacgacgtc tgccagacca ttctggtggc
     3661 cgcagccgaa gacgaggagc agatccggga gaaatacggg gccaacctcg gcttcatggg
     3721 cgtcaagatg gtcaccggtg ggccgacccg gccggattcg gtggccaacg gactcgccgt
     3781 cgtgaaagaa gatgcccagt tcgtcgcggt acacgacgcc gcccgaccct gtacgaccga
     3841 tgagatgatc gacgcggttt tcgccgaggc ggccaaaacc ggcgcagccc ttctcgcagc
     3901 cccgctaagg ggaaccatca aaagggtcag cgagagcaag gttatcgacg agacggtttc
     3961 ccgccagggc ctgtgggaag cccagacacc ccaggtcttc cgccggaacc tgatcatggc
     4021 tgcctatgaa aagcgggctg aattccgcgg cgagctgacc gacgacgccc agctcgtcga
     4081 agtcatgggg catccggtcg cggtcgtcga gtgcgacttc accaacctga aagtcaccac
     4141 ccgcggcgac ttgaccttgg ccaacagcat tgtgaagttt cgcccgaaac ccaaacccaa
     4201 aggcccattg ggcgcctttg aagaagccca atggtaagga ccccagaacc gcgaaaacgc
     4261 gcccattcac ttgggtggtt cttgacgatt cagcgggcgc aagcgtactc tttccctgta
     4321 gcgggcggaa ccttcgcacc gaatagtatc atgttaatct cagagacgca ggaggcgtga
     4381 tcatgcgaaa gcacagcacg atgcttcttt tccagctcgg cgcggtcgtc agtctgttat
     4441 gggtgctgac gttgcctgcc gggtgcaaga ccgattcact ggcttccgag aagatcgagc
     4501 gtccgcctgg gccaatgacg tcgctcgaaa accctgccgg ggctaatttg ccggagatct
     4561 ccattgccgg ccgggacgaa ctggacatcg ttgaagagct ggtgatgcac cgcacacagt
     4621 acgcccgcct gcttcgggcc ttggtgattt actacaacga acacggaatg tccgacaagg
     4681 ccatgtgggc cagaagcgag ctggccgacc tccagcgggt caaaccctac gactacatcg
     4741 ccgaggcggc tacgcccgtg gaaacgttga ggcccaagga ctccattccc gaggccaaca
     4801 agttgttcga ggagggcatg gccctcctga aagagggcgg tcatggtacg cccatcttct
     4861 acaaccagga aatcatgaag aaggctttgg ccaagtttaa ggaactggtc gagaaatacc
     4921 cgaccagcga caagatcgcc caagccgcct tctacatcgg tgaaatccac aaggaatact
     4981 tcgaagaaaa ggacaacgac atcgccttac tgtggtacaa gcgggccatc gactgggacc
     5041 ctaatatcca gttgccggcc aggttccaga tggctgccgt atgggactac cgtctgcaca
     5101 accgcgacaa ggccctctac tggtaccagg aggccatcga gaaggagcaa ttcaacaggt
     5161 ccaacgtgct ctgggccaag agccggataa gggagctcac accggcgcgc gttgaaaccc
     5221 agccgacgga ggaggaacga tagatacccg aaggtaagtc cgaatgcatt ttttctcctg
     5281 accgtcgacc tctcaccgct gtaaagcatg cccggtactg gcagtcccgg cgtctagctg
     5341 cgtggtcttc tccctgcccc tctgggaccg ccgaaggttt ggtcgcccaa gaatcagtga
     5401 gaacgtgcgt ccgagccact cctcaagggt accagagata tggagaggca cgctgcaaca
     5461 accgcaacct gcgcgcccgg tttgcccgcg aggaccatcc tcctgtacca tgtacccccc
     5521 gatttgatgg aggctcggat gaaactggcg acatgcagcg aaccttggcg ggacgttccg
     5581 atcgaggaag tcttcggcat cgcggctcgc atcggctttg aaggagtcga gcttgccccg
     5641 tttaccatcg ccgagcacgt ggaccagatc tcgtccgacc gacgaaagac gatcgcccgg
     5701 gcggcagccg atgccggggt atcggtggtc gggctgcact ggctgttcgt gtcgcccaag
     5761 ggcttgcaca tcaccacccc agacgacgca gtccgccgca agtcggccga ctacctgagg
     5821 agcttggtcc atttctgcgg tgatgtcggc ggcaaggtca tgacccttgg ttcaccgaat
     5881 caacgcagca tcgtcccacc aacgaccttc gacgagggct ggaaacgggc gaaagacgta
     5941 ctggcgtcgg tggcccacac ctgcgctgag cgcggggtga ccatctgctt tgaggccctg
     6001 agccccaaag aaacgaattt catcaatacg gctgaggaag ccgcccgact ggcggatgag
     6061 atcggccacc cgaacatcga catcatgctc gacatgaagg ctatggcctc catgccggat
     6121 ggcatcgagg gtacgatccg tcggttcggc aagcgggcca ggcatttcca cgccaaccat
     6181 ccttcgggca agggagtggg aatgccgttg gcctcgggcg acggcgagcc gctggacctc
     6241 aaatcgatcc tcaagactct ggcggaaact ggctttgatc gttgggtttc ggttgagccc
     6301 tttgactata aaccggaccc gacgacggtg ggcgagcatg cgtataggac gctgaaagcg
     6361 gcactgcccg cgtgacttca tgttcgtgag acaagaggag acaaagacgc atatggtaaa
     6421 caagatgatg ggaattgcgt ggatcgccgc gattgttgca ggaatgggcc tgacagcctc
     6481 aacagcacgc gcggacaagg tgacgatcac cgtcggcgac aaggatattc ccgccggccc
     6541 gatgtggttc aagccggcca agcccctgga cccgcagcag tactactatc tttccggtaa
     6601 ggacgccgat ctgctcgtgc aaatcgacaa ggaagatcgg gtatggtggt ggcagaacaa
     6661 gccgctcaag gccgggcaga cgtacacgtg ggaactcctc gtggccggca cgggcgtcaa
     6721 cccggaaccg ggcggcctcc ccgcccgcgt ccgcaaagcc ggcgacgaac aggtggacat
     6781 caagatctcc ggcaagccct tcaccaccct ggtattcaag aagagtgatc ccaaggttta
     6841 cctgtatccg gtcatcggcc cgacgggcga gccgatgacg cgcgatttcc ccatgaagga
     6901 caaccctgcg gaaaaagaag ccaagcgcca ggaccacccc caccaccgct cattgtggtg
     6961 cgcccacggc gacatccgca cggaagacat gaccaagccg ggatccaact actggaatga
     7021 agtcaaggat gcgccgatcg agaaagtgcc gcaccaggtt ctcaccaaga tcgtcggcca
     7081 ggccagcggg ccggtattcg gtcaggttat cgccgaaatc gactggatca acgagaaggg
     7141 cgagaaggac ttctccgaac agcgatcgta catgtttctc gccggtgagg atcttcgcat
     7201 catcgacgtc aggagcgtct ttaagttcga ccagaaggac gttaccttcg gcgacaccaa
     7261 ggaaggcggc atcgtgtccg tacggacggc catcaccatt gacgaaaagg gcggcggcaa
     7321 gcggtacaac agcgccggca agagcggcga agacacctgg ggccaacccg ccgagtggtg
     7381 cgactacgtc ggaaaggtta atgacaaggt ggtgggcatc gcaatcatgg accatccgaa
     7441 gaattacggg catccgacgc actggcatat tcgcgactat ggtctgtaca ccgccaaccc
     7501 gttcggcctg aaggacttca ccaaggatgc cgccaaggac ggttcacata cctggaagaa
     7561 gggcgagtcg gtcgagttta actaccggat ccttttgcat cagggtgaca cgcaagccgc
     7621 cggcatcgcc gatcagtgga gactctacag cgacccgccg aagttcacgg tcacggagaa
     7681 gtgatcaatc ctctccggtt gttcagactt cggacgcgtc tattccgtaa agtcgaacca
     7741 gagacgactg aagatctcat cgaacgaccg gaaaaacgcc cacgacgact ccggaatcgg
     7801 aacgtcaccc caatccagcg gtgcttgctg gggcacgagc ggctccgccg gcggccgtcg
     7861 cggcccgatg atatcgggtg ccgcggcgga ccgcaccttt atccgacgag cctgttgcac
     7921 ggaacgatcg taggcgtcac gccgtcgagg atcgctcagg acggcgtacg cctcttgcac
     7981 ctccaggaac ggctccacat ccggcccgta gtgatccggg tgtagctcca gcgcctttcg
     8041 acgatacgcc tgcttgatct gtgccggcgt tgcatcgacg gtaagcccca ggacgacgta
     8101 gtagtccttc gccatgccga caccccgatc accgacctcc gttgctggat ccccacatgt
     8161 cccgggatca gccgctcttt acggcaatcc gcttgggctt cgcgtccggc cgtttctgaa
     8221 gagtcacggt caggaccccg ttcctgtaag tcgcgtcaac cttgttcgga tcaaccgaac
     8281 tcggcagttg aatgctgcga cggaagctgc cgaagctgcg ttccacgtag tgatagttgc
     8341 gcttcttttc ctccttctcc tgtttcttct caccgctgat cgtcagcatg ttgtcgctga
     8401 cgctgatctc cacgtccttg ggatcgacgc ccggcagttc ggccgtcagg gtcacgtcat
     8461 tctcagactc ggccagattc agccgcaacc ccacgccgac accggccggc atcaggtcgg
     8521 cgaggctggt gctccatgga tcccgccaga accgctcaaa gagctggtcc atctcacttc
     8581 gcaaacgagc cagtgggggc gcttcggtcc tcgaaccgtt tctggcttca cgcttgtttc
     8641 gccaaggtat caggttcatg attcaacctc ctctcacgac gccttgaccg tgatcttcct
     8701 gggcttgcag gcctcgctct taggcagatg cagctccatc actccatcct tgagccgtgc
     8761 ctcgatcttg tcggtctcga tgccttcgcc aagccggaag gaacgataaa agtcacccac
     8821 tccgtactca tgaagcaggt agttcgtctt atcctcgtcc tgccggggat caaccctgcc
     8881 gttaatggtc agaacgccct gctcgtactg gatgtcgatc gcatccggag caacgcccgg
     8941 catatccgcc agaacaagga actcgtccga cgtctcaata atgtccacgt tcggcatata
     9001 cgtgcgacga tcgcgtgccc gttcgacttg ggcaagcttg tcttgctctt tcttgactgt
     9061 caaagcggtg ctcattgctg atcctccttc tcaaatggcc tcgtttctcg ttctccgcaa
     9121 ccgacggtta actcgtcttg acggcgatct tcctgggttt cgcctcgtcc accttcggca
     9181 aagtgacggt cagcacgccg tccttcaatg tcgcctggac cttctccgca ttcacctcgg
     9241 caggaagcgt aaccacgcgg gtgaactcgc cggtgccgcg ctcccttcga tgataagagg
     9301 cgccctcgct ttcccggggc ttgcgcgttc ccttaatggt cagctcgttg ccgactgcgt
     9361 aaatctcgat gtcgttctcg ctcactccgg gaacctccgc ctccgcgtac agggcctcgc
     9421 cgtcttccca gatgttgaga gctggaaacg cggtcagccg gcttgtccag ccgccgttga
     9481 acggaaaacc atacccttcg aagatccgct ccacctccct ccgcaactca ttcatgactg
     9541 caaaaggatt catcgttcct gcaaacatcg cgatacctcc tggtccttcg gacctctgtc
     9601 agtctgcaag cacgtccgcg tggccttccg caggccgggt actccgcggt tgcaccggcc
     9661 gatccgagcc cgatgctgga agcacaaaga ccgctcggcg tcgctgattg tttcgggaag
     9721 ttcctgcccg ccgcgagaag agcggccggc acagtggtcg gcgacgcgtg aaatccgtta
     9781 ccgaaaaagt cttacaggct cggtcacatc gccactcctt cgagcggacc tgcttccgca
     9841 ccatgcttct tgggccgttt caacgtgccg ctgaaaatca catgcaaacc ccgtgccagt
     9901 cgaaccggcc aaccatgccg atcatcataa gtaattcgaa acaggcaact tagggcaagg
     9961 ctgcccacag cgagacacgc gatacggccc gagactctgc caatctcgcg ctgccagttt
    10021 tgcgggcccc tcggctaagc tcagccgggc gggagttcgc gtgcgtttcc aggacggtga
    10081 tgccgtgctg acaattcagg gatacagtca cccactcatg tagatgaggg ggtcagtagt
    10141 tcgcgttatc gcacgtctgg tcggctgcaa tgccgggtcc gctcgcacat gcttcgaact
    10201 tgtcgagatc ggtgttgtcc acgtccccat cgccatcgtc tgggggattg aagcgttggt
    10261 cagcattcat gtcgtcgcgg tcgaagcatt cacagtcggc gaacagcccg ccgccggctc
    10321 ccgaaaagca ggcctggaaa accgcgaagt cagcctgatc gacgtcgccg tcaccgtctg
    10381 cgtccgcgaa ggggtcattg caagcacatg ccgccgaggc cgcgcagtcc ggatcggcac
    10441 aatcggtcag gctgtccagg tcgtcgtcga tcagattgta acagttgtac tccggaacca
    10501 ggctggcact cacgttgtcc aggtcgatgt acgaggtgta cggggccggc ccgatcagac
    10561 gttgctcggc tccgaagccc acgatgcccg aggtgtattt gtacacggtg tcaacgcaat
    10621 agacgtccca tgtgctcggc tcgggcgtgc cgtcgtccgg ccagatacga cctctgacaa
    10681 gtacagcccc cgcaccatca tccagggcct cgaaacgcat ccaccagtcc ttcgtcggat
    10741 cgaaaggatc gaaacgtatc gaccccggag tggggctgca gagatcgtga ggtccgctgg
    10801 gatccagata ttgtatcgcc atttccagcc gattgttggt gggaggattg gcattcgggt
    10861 tggccccggc gtcgcctaca tagacccagt agcccttgaa gtgggcgccg gtcttgtcgg
    10921 atgagaattg cggatccatg ttgaccgcca atgctacaaa aaaagtgttc ttgtctggag
    10981 tggcaaacat gtgggggttg gtggaggtct tgatcagcag cttggccgac acgcccccat
    11041 tggcgttgct gaactccgtg ccatactcgg agaagagacc caccgccctg tcgatggagg
    11101 aagtgaagct gaggcgaagg gcgggtgaac agctggggca aggagtggcg gttgtcggaa
    11161 agaaggtcac aattgccccg tccgtctgcc aggaaatgta gtcccagggc accaagcctt
    11221 cgaacgtgtt gtcgtcgaaa gtatgcagcc aaccgggggg gtcagcccaa gcgggcggaa
    11281 ctgccaacag agcgacactc agaaccgccg gcccgatgaa cacgtgcttc gggttcatgc
    11341 cttcttcctc ccagataaag gttaatcgaa atgcgtttcg ttcgctcacc gccgacacaa
    11401 tagagacgtg cagggaagct cgcggggcca agacaagaac ccgaccatga aatgggagcg
    11461 ctcactcccc tacgaagtcc ggtctctatt acatcgtaac ccgtcccaag gttgcatgca
    11521 atcaccttct ctccgacttc ctcgtgccgc gcatcgagca gcgtatggtt ggtgctcttg
    11581 acaagaccgt cagactgatg ctgactcgcg caaggaatcc gctgtttttt ccccagcgga
    11641 cccctccttc catgcagacc gaccttcgga gcggatgctt catcatcacg gtccgcatcc
    11701 gcatcgatgt ttgtggtggc gatccggcca ggtttccgcg taagccattg cgatacaatt
    11761 acttgggttt gtttccggga ccgccccaag tccgagaagc ggctgcaggg aaagaacgat
    11821 ttgcgcttga ggctgacata ggccgcggat atgatgcagt cagtcgatca ccgactccca
    11881 cgtcatggat ggggcgcgat tgtccgtttg aagtcgcagg gcggttgaag gtgcgcaaaa
    11941 gcaactcccg gaaaccggag agggcttgaa gaccctttca gaagtctgcg tgttggggat
    12001 aggttgtctt aactactagg aggagaacgt tatgctaaga ctctttgcag tggtagatct
    12061 cgtcgccgtg ctcgtcgtgc ccgcagtcgg ggcataccag tacgatttca cccttgacca
    12121 gagcaggtcg aacatcacga tcacgcttcc gatctttgcc ggcggcgcga ccgcgaccag
    12181 tccgctgagc gggacgtaca gcatgatgct ggattccccg tccggcacgt acaacacccg
    12241 ggactggaac gtacaggcca gtctgaagac catcagtgcc acgaacacgg ctcagatgat
    12301 cttgatctcc gtgccctaca acatctacgt ccaggttgat ccgggcgact tccgcgccat
    12361 ggactggaat cagaataagg gggccatccc gagcacgaca cttgcgggtg ggcccaacat
    12421 ctcgagtggc gacctgagta ctgatatcct gctctcgatc tacaatgagg tcactcacag
    12481 ttatgtcacc aggaacgggg ccgcgggtga atggttcatg atgaactgga ccctccaggt
    12541 ttccgacgac gactggctgg gagtggcagg cacgggcgac gtggagtcgt gcgtgaccgc
    12601 gacttactac gatcagaacc tcatcaagta cgtcaccaag ctgtacggcc ggggcacgct
    12661 gatccccgaa ccggccactt tggctctgct cgggctcggt ggcttggcgc tgctctgccg
    12721 ccggccggtg tgagcatgcc cgccaggcgg ccggcgcgat atgaccggct gttagtctct
    12781 tccgtcgcaa ctggtgcgca ttttgcgcgc atgtttctcg atcgcagagc acttgggttg
    12841 cggcccgcaa aaaggccctt cgccgcaggc cacaagctct atgagccaac ggttcgcgtg
    12901 gtgtggacta ctcgttttca gggccctttc acacatcttg ctgttcagcc aggccccggg
    12961 cacaagaacc gccagccgta tttcaaaacc ttggtgctct gggcagtcgg tcatcaacga
    13021 tcactgtctc tcgatgggag ggaatgaagc cctcgccttc a
//
LOCUS       DAOTPW010000162        12845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4703_length_12845_cov_5.175841, whole genome shotgun sequence.
ACCESSION   DAOTPW010000162 DAOTPW010000000
VERSION     DAOTPW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12845
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4703_length_12845_cov_5.175841"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            564..830
                     /locus_tag="P5316_14460"
     CDS             564..830
                     /locus_tag="P5316_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRR86206.1"
                     /translation="MSEQLVGKVTHYFGKPRVAAIEITNGELRVGDTIHIKGHTSDFT
                     QRIDSMQIEHAPVECAKVGDSIGIQVAEHAHEHDQVYLVTPDGQ"
     gene            complement(940..3711)
                     /locus_tag="P5316_14465"
     CDS             complement(940..3711)
                     /locus_tag="P5316_14465"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR86207.1"
                     /translation="MTARSILPAISVLLTSSAATQAATPMTFDTADSVKSWTFSNGPE
                     FPGATGRIEWDAGNGHDKAGCLRLSFSFEGGGNYIQAGCLLPRENDFKTARLWLKKPA
                     GNRVTFRGIDSSSQTFQKSVDFSFDDWQQFELDLTRWTSSFGGANDQKVHWPMRGFAV
                     LIENTATGKEGSLLVDDLSFAADAPSPTETVTAYTAWNQHSSIGFRADGGPGNAFDKG
                     TWNYTFSGQSSPSIHTEFSLLGRPAGMKLFFESDASGHEVVVTLGSHFQNFRKQVGRL
                     TEKGPQIIDVPLGNLEGWEYFGGENNGQPQFPLRITQIALKRTEGGPEKGTIRLERID
                     AQTPLPADQKVVLLPDVKQTGNRTLFKVEIQNLRKTQAQGELVGRFRMGNGVDSDIRK
                     VTLPANGAAPTIIELEPRAIGNFNVVEGTFWWVEGDSISSPASIGASTVPTEAGSTAL
                     DPSSPFGVGLYLYRWHGHPQAKEKMNELATLARHAGVKWTREEIDWHRTEPKKGEFDW
                     KFYDDLVDVAHANGISIYGLLCYWSYYSQKDTPEGVEEYCQWTRQVVRRYKDRIKHWE
                     IWNEPNIFFWSGPKELYFSMLAKAYDAIKAEDPEAQVLGCSTAGIDVDFIKQAMAAGA
                     NFDALTIHPYRGELNDLQFIKELRDVKELVGGRPVWITEMGWPSDRFGGTTERRQASF
                     VARTYLTSVAGGAVANVSWYDFRNDGNDPYYNEFNFGMVRNDLRPKPAYQALATIANT
                     LAGMKVTEHIDLGPDAYAFRFSDGKRDVVAACAPQAGRLLAWRGEQHPTVINTFGEKA
                     ACVAAEGLSITTLESGMPVYIRGQAGFEFQPAELPIKLSVDRSTVRPGQTVTIRVEPK
                     ASVAPSSIWLPLWDEPAPAPDAEGTYHLTIPKGAAPGDTELMLEIGWNKLHLLWPLKL
                     SVQPNVLRV"
     gene            complement(3744..7295)
                     /locus_tag="P5316_14470"
     CDS             complement(3744..7295)
                     /locus_tag="P5316_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRR86208.1"
                     /translation="MADGSNRYCEMRRLSVVFACASLALLISLLAWIWLDLTPAWKTH
                     QRNVTETRTALRRLNELASGQVHTKPAPPSESLAIRQVVLPLVRRDLHFTQTATTDRC
                     MTCHAAIDADDLSTHRLATKLTDALLQISERIRRQNLNPRSESQDSRLGGWVTEVPGL
                     PKAQDNSRIWPALSPVDQVSLLGDLVIDANRHLRQSGSSLLNCDTPAAAHPQLDLYVS
                     DRSPHPMKQMGCTVCHEGNGDETDFVLAGHAKISAGTARAAMERPMLPLWGTEAGCAK
                     CHPQISDTTMPAGPAVARRLNRGRVLVERWGCVNCHLIEGLDNAPKVGPSLAKAQITL
                     NERFFGEYVLSPRDLRPWTTMPQSFRQENNIGPAGNDADIRSAAEIDAMWSYLAAISQ
                     DEPSQPGREWSPELQESEVVTAAREQGRRLFNELGCLGCHPPTERRSGPYGLATSRPA
                     DLRHKSGDSNNGTHADLRHSDDVDVLFAPEGTVQAAMVRYAPDLSNMGGERRGGPWVR
                     EWLADPGRYSPGARMPRMRLTTQPTSSPAPAADELDEATALALYLSNLETGEMQATRP
                     ATTGRASSENYKAVCEAITREVLAAGESGEAVISDRLQAATSGRKPAADRVAAMTPVQ
                     QQWAFVGQRLLAHYGCAGCHQIPGLERAPRIGPELTRWADKPAGQLDFGFFDPMLRRT
                     KTDTRFQRLYPTTGEQPWLRWIPDNLTVQIERTHASFVWHKLRNPRMWDRGRDKPPLT
                     RLRMPNFFMDNTQAGSLATFVLSRRAPNVDASLIISDHSPAGVAARGRNLARSLNCTA
                     CHAIDGNGAAIHAYYWKPAGDELHFQAAEAPPRLPGQGARVTAQWTYQYMRDVTTIRP
                     WLSVRMPSFNLSTEQTTLLTEYFSAVSGEQSRWLGKKLAAIKKGASPGQNSPGRDSLG
                     QDSPGQNSPGRDSPGRDSHLGPLSAASVSLGRDSHLGPLSAASVPDRIPSVADVLFPT
                     SGRDTTEWENAVTAVDRLRRFALSHGAIPAQWLDPRETDRSTFARACDQALADAGFLQ
                     QVYSIDHPFLDTPAETATDDQVADGQTLFQELQCLQCHVFGSPAVPGANAHPTAPDLQ
                     RANRRLRRPWVSMWLRNPRRIQPGTRMPGFFGDGQASAFADYPPEDRAGLQRRLHDKS
                     LLDDGPAQIQAIIAFVYDASSRQVDVVREAQTQPADN"
     gene            complement(7322..8152)
                     /gene="kdsA"
                     /locus_tag="P5316_14475"
     CDS             complement(7322..8152)
                     /gene="kdsA"
                     /locus_tag="P5316_14475"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRR86209.1"
                     /translation="MTDLSPVPSIEVAGIRIGPGRGLVLIAGPCVIESREHTLRLAEA
                     IKAVADKVGIPLIFKASFDKANRSSISSFRGPGLEVGLAILSEVHMTLGIPVTSDIHT
                     PEQASAAGQVLDVVQIPAFLCRQTDLLVAAADTGRAVNIKKGQFMSPEEMGLAVGKVR
                     QAGNERVLLTERGTFFGYGRLVNDMTSIAKMAHFAPVVFDATHSCQLPGAAGTQSGGQ
                     RELVPTLANAAVAAGAHALFLEVHDDPPKAKSDSATVWPLEKLAGLLTQCKRISDAVG
                     "
     gene            complement(8163..9083)
                     /locus_tag="P5316_14480"
     CDS             complement(8163..9083)
                     /locus_tag="P5316_14480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRR86210.1"
                     /translation="MTYARTRAVLIVNPIAGAGTDRRPLSQMIALAKQAGIEIHYQVT
                     HGPGSARRLAAEISADPRNASSIRAVMAVGGDGTVHEVADGLAHGPLPLVVWPTGTEN
                     LFAKSFLFHANPRAALDCLLHGQVRLIDLGQANGRSFLIVAGVGFDAEVVHRLAGTRT
                     GHISHLTYSTPLWRTFWEHRFPRLRVYDGNSLLWEGQGLVFVGNLPRYSLGLRVIRDA
                     ICDDGLLDLCVFPCRGRLRLIGHSLRTMLRTHVEHGGVLYRRLQNVRVESDDPVPVEL
                     DGEAAGHLPLDLKVRPRALRVLVPPEATRL"
     gene            10167..11114
                     /locus_tag="P5316_14485"
     CDS             10167..11114
                     /locus_tag="P5316_14485"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HRR86211.1"
                     /translation="MMDNVLALIREKRLDEAEAELSRLQPGDANYHCARGLLLKAKGM
                     TQEAIDSLEAACRMDASSLEAVFHAARIQDLYGDDEAAIENYRKLVERPTVPVNVLLN
                     LAVLLEDHDEYEEALKLVERVLREYPNHARARLFRKDIKSSMNMLYDETQEQSREHQS
                     ALLETPITDFELSVRSRNCLKKMNINTIGDLLRITEAELLSYKNFGETSLEEIRAMLQ
                     QKNLRIGMLREESQKATRSMPQRQSPPQGSPDALNKPLSEIEFSGRSRKCLQRLGLVT
                     LGDLTLKTEAELLATKNFGQTSLNEVKHKLAEFGLSLRK"
     gene            11317..12588
                     /locus_tag="P5316_14490"
     CDS             11317..12588
                     /locus_tag="P5316_14490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRR86212.1"
                     /translation="MGRFEGRQELLGLLIPVLFMTGCDGPSSEPVAAPLRSVATARSA
                     PGLDRTMRVLLAADLPSYQVAVPGLFDLIDADGHKVLCQDAGAPQLTIQFGPGAIAFL
                     ELERVFEVEAVEIVPHGPEPVTVFLGDQRSRYAGTLTVYRYSDDTGALVNSVDVEDYL
                     VSVVASEAPATFHPEALRAQAIVSRTYAWYQKRMAPPGRRWDLMASEGSQVYSGIPWA
                     PAGRLSAEAVRDTQGLVCTWRSPQGERIFCAYFSSTCGGHTQAAGPVKNESTIPPLSG
                     NVRCDYCADSPWFSWGPVRISKKVVTKRLRQKYPRFQNLSQIEEIQVVEQTAQGRPVR
                     LRLLDARKRSIELEAENFRLTVDPTGRELRSTFCKIQSRADHFVFSEGKGFGHGLGLC
                     QYGADGMARAGADAAYILRHYYPQSSLKRAY"
BASE COUNT         2317 a         3612 c         4307 g         2609 t
ORIGIN      
        1 catgccgacc ggtccaggtt gggcgatcaa ggcccttcag cgggtttgcc cgccagaggc
       61 ggcctatctg ttcttcggga aagaccgctg aagcgctctg ctttcggcct gcgtccacga
      121 caaccgggtc agccgcgagg gaatgccgcc gcttctgtca cgggcccggc tatgagggcg
      181 tagctgatag ctcttactac ttgtcagcct tccgcggggg tttcggattg cggcactcag
      241 tggggggtgc aagaaggcga ttggcacgag gccgtgccga cggggtccgt gacacatggg
      301 ggcggaaacg cctttggggc tatttcagaa cggcgaccgt acgggccgct tgtgatgcgg
      361 ccgttttgtg gtccagcttg ccccctgccg actttcgaag ggcggtcaga ggccccatgc
      421 ttgcgcttga ggttctgaga gagccgccca gactatcacg ggcgcgagtg ttacggacgt
      481 ctccggacca gcttttgtct tccggccagc tccggtacaa tgcttcacgt catttcgaga
      541 ggtctttatt aaggaggccc gttatgtcgg aacaactggt cggcaaggta acccactatt
      601 tcggcaagcc gcgtgttgcg gccatcgaaa tcaccaatgg cgaacttcgc gtcggcgata
      661 cgattcatat caaggggcac acctcggatt tcacgcagcg cattgactcg atgcagatcg
      721 agcatgcacc cgtcgagtgt gccaaagtgg gcgacagcat cggcattcag gtcgccgaac
      781 acgctcacga acatgaccag gtgtacctgg tgacgccgga cggccagtga gggtccggcg
      841 ctcggggaga gagggccgcc gggcagctca gtccgccgca gcaccgcctt catggcgaag
      901 actgccgtcg cacccggcga agacgggggc gtgcacaggc tacactcgca gcacgttcgg
      961 ttgtaccgag agcttcagcg gccagagtag atgcagtttg ttccagccga tttccaacat
     1021 caattcggtg tcgcctggcg cggctccctt tgggattgtc agatgatagg tgccctcggc
     1081 atcgggagcg ggcgcgggct cgtcccagag cgggagccag atcgacgatg gggcgacaga
     1141 ggcttttggc tcgacgcgga tggtcaccgt ctggcctggg cggaccgttg atcgatcgac
     1201 agagagcttg atcgggagct cagccggctg gaactcgaaa ccggcctgcc cgcggatgta
     1261 gacaggcatg ccgctttcca gcgtggtgat ggacaggcct tcggcggcga cgcaggcggc
     1321 tttctcaccg aaggtattga tcaccgtggg gtgctgttct ccccgccagg ccagcaggcg
     1381 gccggcttgc ggtgcgcagg cggccacgac gtctcgtttg ccgtcgctga atcgaaaagc
     1441 gtaggcatcg ggtccaaggt cgatatgttc ggttaccttc atccccgcca gcgtgttggc
     1501 gatggttgcc aaggcctggt aagccggttt gggccgcagg tcatttcgca ccatgccgaa
     1561 gttgaactcg ttgtaatagg ggtcgttgcc atcgttgcgg aagtcgtacc aggagacatt
     1621 ggcgaccgct ccgccggcca cggaagtcag atacgtgcgg gcgacgaagg acgcctgccg
     1681 gcgctcggtt gtgccgccga accggtcgct gggccagccc atctcggtga tccacaccgg
     1741 gcgtccgccc accagttctt tcacgtcgcg cagctcctta atgaactgca ggtcattcaa
     1801 ttcaccgcgg taggggtgga tggtcagcgc gtcgaagttg gccccggcgg ccatggcttg
     1861 cttgatgaag tcgacgtcga tgcccgccgt cgagcagccg agcacctgcg cctcggggtc
     1921 ttcggccttg attgcatcgt atgcctttgc cagcatgctg aagtaaagct ctttcggtcc
     1981 ggaccagaag aagatgttcg gttcgttcca gatctcccag tgtttgatcc ggtccttgta
     2041 gcggcgaacg acctgccggg tccactggca atactcctcg acaccttccg gcgtgtcttt
     2101 ctgggagtag tacgaccagt agcagagcag accatagatg ctgatgccgt tggcatgggc
     2161 gacgtcgacc aggtcgtcgt agaacttcca gtcgaattcg cctttcttcg gctcggtgcg
     2221 gtgccagtcg atctcttcac gagtccactt caccccggcg tgtcgggcca gcgtcgccaa
     2281 ctcgttcatc ttctccttgg cttgcgggtg gccgtgccag cggtacagat aaaggccgac
     2341 gccgaacggg ctggacgggt cgagagcggt ggatccggct tcagtcggca ccgtgcttgc
     2401 tccgatggat gccggactgc tgatgctgtc gccttcaacc caccagaagg taccctcgac
     2461 tacgttgaag ttgccgatgg cgcgaggctc caattcgatg atggtcggag cggccccgtt
     2521 cgcgggcaga gtgacttttc ggatgtcgct gtcgacgcca tttcccattc tgaaccggcc
     2581 cacaagctct ccctgggctt gggtctttcg caagttctgg atctccacct tgaagagagt
     2641 ccggttgccc gtttgcttga cgtctggcaa caacacgact ttctgatcgg ccggcagagg
     2701 ggtttgtgcg tcgatccttt ccagtcggat ggtgcccttt tccgggccgc cctcggtgcg
     2761 tttgagtgcg atctgtgtga tccgcaatgg gaattggggt tggccgttgt tctcgccgcc
     2821 gaaatactcc cagccttcca ggttgcccag cggcacgtcg atgatctgcg gtcccttctc
     2881 ggtcaggcgg ccgacctgct tgcggaagtt ctgaaaatgg gagcccaggg tgacgacgac
     2941 ctcgtggccg gaggcatcgc tctcaaagaa aagcttcatg ccggcaggcc gacccaagag
     3001 tgagaactcg gtgtggatcg atgggctcga ctggccggag aaggtgtagt tccacgtgcc
     3061 tttgtcgaac gcattgcccg gtccgccgtc ggctctgaag ccaatgctcg aatgctgatt
     3121 ccacgcggtg tatgccgtaa ccgtttctgt cggcgatgga gcatcagcag cgaacgagag
     3181 atcgtcaacc agcagcgatc cttccttccc cgtggccgtg ttttcgatca gcacggcaaa
     3241 gccccgcatc ggccagtgga ctttctggtc gttcgcgccg ccgaaggacg aagtccatcg
     3301 cgtcaaatcc agctcaaact gttgccaatc atcaaagctg aagtccacgg acttctggaa
     3361 cgtttgcgag gaggagtcga tgccgcggaa tgtgacgcgg ttgccggccg gctttttgag
     3421 ccacagccgc gcggtcttga agtcattctc cctgggcagc aggcagcccg cctggatgta
     3481 gttgccgcca ccctcgaacg agaaggacag cctgaggcag ccggctttgt cgtggccgtt
     3541 gccggcgtcc cattcgattc ttcctgttgc tccggggaac tccggaccgt tgctgaaggt
     3601 ccaggacttc acggagtcgg cggtgtcaaa ggtcatcggt gttgccgctt gggtggcagc
     3661 gcttgaggtg agcaggacgg aaattgccgg aaggatagat cttgctgtca tggtttgctc
     3721 catcgccagg atcgaatggt ttctcagttg tcggcaggtt gtgtctgcgc ctcgcggacg
     3781 acgtcgacct gccgcgacga ggcatcatac acgaaggcga tgatggcctg aatctgcgcc
     3841 gggccgtcgt cgagaagcga tttgtcgtgc aggcggcgct gcaagccggc tcggtcctcc
     3901 ggcgggtaat cagcgaaggc acttgcctgg ccgtcgccga agaagccggg catccgcgtt
     3961 ccgggttgta tgcgtcgtgg gttgcgcagc cacatcgaca cccagggccg tcgcaagcgt
     4021 cgatttgccc gctgcagatc gggggcggtt ggatgggcat tggctcccgg cacggcgggg
     4081 ctgccgaaca catggcactg gaggcactgc aactcctgaa agagcgtctg accgtcggcg
     4141 acctggtcat ctgtggccgt ctcggcaggg gtatcgagga acgggtggtc gatcgagtac
     4201 acctgttgca ggaatccagc gtcggcgagt gcttgatcac aggctcttgc gaaggtggag
     4261 cggtctgttt cacgaggatc cagccactgg gccggtatcg cgccgtgcga caatgcgaaa
     4321 cgccgcagcc gatcgacggc cgtcacggca ttttcccact cggtcgtgtc tcgaccgctg
     4381 gttgggaaaa gcacatcggc gacggaggga atgcggtcag gaactgatgc ggcggaaaga
     4441 gggccgagat gggaatctcg gcccagcgaa actgatgcgg cggaaagagg gccgagatgg
     4501 gaatctcggc ccggcgaatc tcggcccggc gaattttggc ccggcgaatc ttgccccagc
     4561 gagtctcggc ccggcgaatt ttggcccggc gaggctccct tcttgatagc tgccagcttc
     4621 ttaccgagcc acctcgactg ttcgccgctg acggcggaga aatactcggt cagcagcgtg
     4681 gtttgctcgg tgctgaggtt gaagcttggc attcgcacgc tcagccacgg tcggatcgtt
     4741 gtgacgtcgc gcatgtactg ataggtccac tgagccgtca cgcgcgcccc ctgaccggga
     4801 agacgtggcg gagcctcggc agcctgaaag tggagttcgt cacccgccgg cttccagtag
     4861 taagcgtgga tggcggctcc gttgccgtcg atggcgtgac aggcggtgca gttgagcgat
     4921 cgggcgagat tgcgacccct ggcggccacg cccgctggtg agtggtcact gatgataagc
     4981 gacgcatcga cgttgggtgc ccgcctggac agaacgaatg tggccaggga accggcttgc
     5041 gtgttgtcca tgaagaaatt gggcattcgc agacgggtca gcggcggttt gtcacggccg
     5101 cggtcccaca ttcggggatt acgcagtttg tgccacacga aagatgcgtg cgttctctca
     5161 atctggaccg tgagattgtc cggaatccag cggagccagg gctgctcgcc ggttgtcggg
     5221 tacagtcttt ggaatcgcgt gtcggtcttc gtacgtcgta gcatcgggtc gaagaagccg
     5281 aagtcgagtt gaccggccgg cttgtctgcc caccgggtca gttccggtcc tattcgcggg
     5341 gcgcgctcga gaccgggaat ctggtggcaa ccggcgcagc catagtgagc cagcagccgc
     5401 tgcccgacaa aggcccattg ttgctggacc ggcgtcatgg ctgcgactcg atcggccgcg
     5461 ggtttacgac ccgaggtcgc ggcctggagc cgatcggaga tcacggcctc tccgctctcg
     5521 ccggctgcga ggacttcacg tgtgattgcc tcacatacgg ctttgtagtt ctccgaggat
     5581 gcgcggccgg tcgtggctgg tcgcgtcgcc tgcatttcac cggtttccag gttgctgagg
     5641 tacaaggcca atgcggtagc ttcgtcgagt tcgtcggccg ccggggccgg ggacgaggtc
     5701 ggctgagtgg tcagcctcat gcgaggcatt cgtgctcccg gcgagtatcg acctgggtcg
     5761 gcgagccatt ctcgaaccca cggcccacct cgccgttcgc cgcccatgtt ggacaggtct
     5821 ggtgcgtaac gcaccatggc cgcttggact gtgccttccg gcgcaaacaa gacgtcgacg
     5881 tcgtctgaat ggcgcaagtc cgcgtgggtt ccgttgtttg agtcgccgga tttgtgcctc
     5941 aggtcagccg gccgtgatgt ggccaagccg taaggtccgc tgcgacgctc ggtagggggg
     6001 tggcatccca ggcagccgag ttcgttgaac aaccgacgac cctgttctct ggccgctgtc
     6061 acgacttcgg attcctgcag ctcaggagac cattctcggc ccggctgcga tggctcatcc
     6121 tgactgattg cggcaagata cgaccacatg gcgtcaatct cagccgcgct tcggatgtcc
     6181 gcatcgtttc cggccggccc gatgttgttt tcctggcgga atgattgcgg catggtggtc
     6241 cagggccgca aatcccgagg cgacaacacg tattcgccaa agaaccgttc gttgagggtg
     6301 atttgggctt tggccagcga gggcccaacc ttgggcgcgt tatctaagcc ctcgatcaga
     6361 tgacagttga cacatcccca tcgttccacc aggacacgtc ctctattaag ccggcgggcg
     6421 acggcggggc cggcgggcat ggtcgtgtcg gagatttgcg ggtggcactt tgcacagccg
     6481 gcctcggtgc cccataacgg gagcatcggc cgctccatgg cggcgcgggc cgtgccggcc
     6541 gatatcttgg cgtgcccggc gagcacgaag tccgtttcat cgccgttgcc ttcgtggcac
     6601 accgtgcagc ccatctgttt catcgggtgt ggcgagcggt cggagacgta gaggtcgagt
     6661 tgggggtgcg ccgcggcagg ggtatcgcag ttgagcagtg atgagccgct ttgccggaga
     6721 tgcctattag cgtctatcac caaatcgcca aggagcgaga cttggtcaac tggactcaac
     6781 gcgggccaga tccgactgtt gtcttgggct ttggggaggc caggtacctc agttacccac
     6841 ccacccagtc gtgaatcctg agactcgctc cgcggattca ggttctgccg tcggatacgc
     6901 tcgctgattt ggagcagagc atcggtcagc ttcgtggcca gtcggtgagt gctgaggtcg
     6961 tcggcgtcga ttgctgcatg acaggtcatg cagcggtcgg tggtggccgt ctgtgtgaag
     7021 tgcagatcac ggcggaccag agggagcacc acttggcgga tggcgagcga ctcggacggc
     7081 ggcgccggct ttgtgtggac ttgccctgag gccaactcgt tcagtcgtcg cagagcggtg
     7141 cgagtctcag tcacgttcct ttgatgagtc ttccaggcgg gcgtcaggtc cagccagatc
     7201 cacgccagca ggctgatgag cagtgcgagg cttgcgcaag cgaacaccac gctgagccga
     7261 cgcatctcac aatagcggtt gctgccgtct gccatgggcc atcgcgttag aagagtgccg
     7321 gtcagcccac agcgtcggag attcgcttgc actgggtcaa cagcccggcg agtttttcga
     7381 gcggccacac ggtggccgaa tcacttttgg ctttgggcgg atcgtcgtgg acctcgagga
     7441 acagggcatg ggccccggca gccacggccg cattggccag ggtcggcacc agctctcgtt
     7501 gtccgcctga ctgagttccc gccgcacctg gcaactggca agaatgggtt gcgtcaaaga
     7561 cgacgggagc gaaatgggcc atcttggcga tggaggtcat atcgttgacc agccgcccat
     7621 atccgaagaa ggttccccgt tcagtcagga gtacgcgctc gttgccggcc tgacggactt
     7681 tcccgacggc caatcccatc tcttcagggg acatgaactg tcccttcttg atgttgaccg
     7741 cgcggcctgt gtcggcggct gcgaccagga ggtcggtctg gcggcagaga aaagcgggaa
     7801 tctgcactac atccaggact tgtccggccg cagaggcctg ttcgggggtg tgtatgtcag
     7861 aggttaccgg tatgccgagc gtcatgtgga cttcggaaag gatcgccaag ccgacctcaa
     7921 ggccgggtcc tctaaagctg cttatgctgg agcgattggc cttatcaaag ctggctttga
     7981 agatcaatgg gatgccgacc ttgtcggcga cggccttgat cgcctcggcg aggcgaagag
     8041 tatgctcgcg agactcgatc acgcaggggc cggcgataag aaccagcccg cggccggggc
     8101 cgatacggat gccggcgact tcgatgcttg ggacggggct gagatctgtc atggtgtgga
     8161 ttctacagtc ttgtcgcttc aggtggaaca agcacgcgga gggctctggg gcgaaccttc
     8221 agatcgagcg gcagatgtcc agcggcctcc ccgtcaagct ccacaggaac agggtcgtcg
     8281 gactcgacgc gaacgttttg gagacgacgg tacaagacgc cgccatgctc cacgtgcgtg
     8341 cgaagcattg tccgcagcga gtgccctatc agccgaagcc ggccgcggca gggaaaaaca
     8401 cagaggtcga gaagtccgtc atcgcaaatt gcgtcgcgga tgactctcag gccgagcgag
     8461 tagcggggca gattgccgac gaacaccaga ccctggcctt cccacagcag cgaattcccg
     8521 tcgtagaccc gaagtctcgg gaaacgatgt tcccagaagg ttctccacaa cggggtgctg
     8581 taggtcaagt ggctgatgtg accggtgcgc gtgccagcaa gccgatggac gacttcggcg
     8641 tcaaagccga ccccggcgac gatgagaaac gaccggccat tggcctgccc aagatcgatg
     8701 agtcggactt ggccgtgcag gaggcaatcg agggcggctc gggggttggc gtggaacaga
     8761 aagcttttgg caaagaggtt ttcggtgccc gtgggccaga cgacgagggg caaaggaccg
     8821 tgggccaatc catctgcgac ctcgtgaacg gtcccgtctc cgccgacggc catcaccgct
     8881 cggatggagc ttgcgtttct cggatcagcc gagatctcgg ctgcgagtcg tcgtgcgctg
     8941 cccggcccgt gggtgacctg gtagtgaatt tcgatgccag cctgcttcgc cagggcgatc
     9001 atttggctta gcggcctgcg gtccgtcccc gcaccggcta tcgggttgac tatcagaacg
     9061 gccctcgttc gcgcgtaagt catttccagg gaaagcctta aaggcctgga gcggttttcc
     9121 atggcgaagg cagaacggcc tgtcgcttgc cttggacccg cagagtgctt gggtttgcgg
     9181 ctattctcgc ctcaaatgag gtccatcagc aagatccgga ggccattggg gtggaaacaa
     9241 gggatgccgg ggtgaacggg caatccgtcg gcaagtcaac ccgtgggggt gcagaacggt
     9301 tcaggcatgg gggtacgagc ggctattgga gaggttgcaa gcgactatgt ctcgattgac
     9361 ctccctgaag tcgattggga acgggtcaga cagcgggagc gggtgattcg tgacggtcgc
     9421 gggtttgaga caggcgcctc cggtgtttgg gaccctacct ttcgccactt gccgaggggc
     9481 gacaaagggg agtacaggtc aaggggtagc ccagcgcggt tccgggcgaa tttggtcacc
     9541 ataagatact tgctgtcaat aagttatatc gtttcttggg ttcgtttgac gcggcggtct
     9601 ggcaactgcc gaagtctggc ggggaaatta gcggtgcttg tgcggaggtt atccattttg
     9661 acttttgggt tcgttcggct gggagtgggg aaaaaggcaa gttgtcaccg gccgggcaga
     9721 ccgtggagtg aggctgctta catgtcaaag agcccggaac cgcaggcgcc cgccaagtgc
     9781 gggcagagga tctctgctga tcccggtgat gacataacac gatggcatac tccggcggga
     9841 tgcgtgtgtg cggagcggcg tgggggaggg atgaccgatg cctggcgccg agggggtttg
     9901 aacaactcgc ccagggggct tgtggccgag gctagaacat ccaaaaatgc atagcgaacc
     9961 ggttacgggg ggtggttgtt cgtgcaagcg gctgggctgg ggcgcggggc cggagcattc
    10021 agggcgtggg gcaaggtacc ttgcatgatg atgatatatg agtagactct ttaagctttc
    10081 ccggggtcat ggttgtgtag cagcggggcc gacccggggg aatgcgctgg ttctcacacg
    10141 cgataatacg gaacaacata ggagaaatga tggacaacgt cctcgcgttg attcgtgaaa
    10201 agaggctcga cgaggcggag gcggagctga gccgcctgca acctggcgat gccaactacc
    10261 actgtgctcg gggcttgctg ttgaaggcca agggaatgac gcaggaagcg atagattcgc
    10321 tggaggcagc gtgtcggatg gacgcgagca gtctggaggc ggtctttcac gcggctcgca
    10381 tacaggacct ttacggcgat gacgaggcgg ccatcgaaaa ctatcgcaag ctggtcgagc
    10441 gtccgacggt tccggtcaac gttcttttga atctggcggt gctgctcgag gaccacgacg
    10501 aatacgaaga agctctcaag cttgtcgagc gcgtcttgag ggaatatccc aatcacgcca
    10561 gggcccggtt gttccggaaa gacatcaaga gctcgatgaa catgctgtat gacgaaacgc
    10621 aggagcaatc gcgagagcat cagagcgctc ttctggaaac gccgatcacc gattttgagc
    10681 tgagcgtccg cagtcgcaac tgtctcaaga agatgaacat caacacgatc ggagacctgc
    10741 tgcggatcac ggaggcggag ctgttgtcct ataagaactt cggcgagacg tcgctggagg
    10801 aaatccgggc aatgcttcag cagaagaatc tgaggatcgg aatgctcagg gaggagtcgc
    10861 agaaggcgac ccgatcgatg ccgcagcgtc aatcgcctcc ccagggttct cctgacgcgc
    10921 tcaacaagcc gctttcggag attgagtttt ccggccgttc gcggaaatgt ttacagaggc
    10981 tgggcctggt gaccctggga gatctcaccc tcaagaccga agcggagctg ttggccacca
    11041 agaacttcgg gcagacttct ctgaatgagg tcaagcacaa gctggcagag ttcggattgt
    11101 cgcttcgcaa gtgagcaccc tcgtgcggat ggttagagct ttgcggccgg acacctgtgc
    11161 ggcttgcgcg catgtttccg gatcgcagtg cgtttgggtt gcgacccgca gaaaggcggt
    11221 ccgtcagcgt aatcgggcat cgaggctcga tgtgccctgg gggcatcggg gcggcatggc
    11281 cgaccgaggt cacagggacg gcgagaggtg tgaataatgg ggcgattcga agggcggcag
    11341 gagttgctcg ggctgctgat tccggtgctg ttcatgaccg ggtgcgatgg tccgagctcc
    11401 gagccggttg ctgcgccgct gcgttccgtt gcgacggctc ggtcggcccc ggggctggac
    11461 cgtacgatgc gggttctgct tgccgccgat cttcccagtt atcaggtggc ggtgccgggt
    11521 cttttcgatc tgatcgatgc ggacgggcac aaggttcttt gccaggacgc gggcgcgccg
    11581 cagttgacga tccagtttgg gccaggggca atcgccttcc ttgagttgga gcgggtgttt
    11641 gaggttgagg ccgtcgaaat cgtgccgcac ggtcccgaac cggtgacggt gtttcttggc
    11701 gatcaacgtt cgaggtatgc gggcacgttg accgtgtacc gatactccga cgacacgggc
    11761 gcgctggtca acagcgtcga cgtggaggat tacctggtct cggtggtcgc atcggaggcg
    11821 ccggccacct ttcatcccga ggctcttcgt gcgcaggcga tcgtgagtcg gacctacgca
    11881 tggtatcaga agcggatggc cccgccgggc aggcggtggg atctgatggc tagcgagggg
    11941 tcgcaggtgt attcgggcat accatgggct cctgccgggc gactgtctgc ggaggccgtt
    12001 cgggatacgc aggggctggt ctgtacgtgg aggtcgcctc aaggcgagcg gattttctgc
    12061 gcgtacttca gctcaacttg cggcggtcac acgcaagccg caggtccggt caagaatgag
    12121 tcaaccattc cccccttgtc gggcaatgtc aggtgcgatt actgtgccga ttcgccgtgg
    12181 ttctcatggg ggccggtcag gatcagcaag aaggttgtta cgaagcgatt gcgacagaag
    12241 tatcccaggt ttcagaatct cagccagatc gaggaaatcc aagttgttga acagacggca
    12301 cagggtcgtc cggtccgcct tcgactgctc gacgccagga aacggtcgat cgagctggaa
    12361 gcggagaact ttcggctgac ggtcgacccg accgggcgcg aattgcggag cacgttctgc
    12421 aagatccagt cccgcgccga tcattttgtg ttctcggaag gaaagggttt cgggcacggt
    12481 ttaggtcttt gccagtatgg cgccgatggc atggccaggg cgggcgccga tgcggcatac
    12541 atcttgaggc attattatcc tcagtcgagc ctcaagcggg cctattgatc tttttcgcgg
    12601 acgccggcaa ggggcagccg gggtgagagc aggggcgtgg aatctgcctg gccggaccgc
    12661 ctggcctgca gggatggtga catgacagcc ggatcccgac acgttctgtc cgcactggtt
    12721 cgcatcgcca tctgcgtttt tgcggtgtac tggctggctc gcgcgaccga atgggatcag
    12781 ttcaagcaag ccatcctggc ggcggactgg ggaccggttg tgctgagctt cgcggtgttc
    12841 ggtcc
//
LOCUS       DAOTPW010000163        12832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4707_length_12832_cov_5.575957, whole genome shotgun sequence.
ACCESSION   DAOTPW010000163 DAOTPW010000000
VERSION     DAOTPW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12832
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4707_length_12832_cov_5.575957"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            514..1365
                     /locus_tag="P5316_14495"
     CDS             514..1365
                     /locus_tag="P5316_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR86213.1"
                     /translation="MHTGSVSHGISCVRRWSGGFTLIEVLVVVAIMALLISILLPSLS
                     EAREQAKRAACLSNLKGLGTAVTAYILNEKERFCWMPKTWLFGGRKGNGEVTWGGWDV
                     PAERRPLNRYVYQGKMRNSMSLEAYRCPSDFGVRLNNEPDSQATKSPGYDVLGTSYQS
                     NHNWASYADSSPDMGPGGEGYAGTALDQRIDYLEDNIVRMMLRKAPTRFIVLYEDPAD
                     WALNTADKFIPNYKVTSWHKKPNYHCMGFLDGHAQYLYVDFKKNSRLRHISGTVDWLA
                     RQDYREQ"
     gene            1372..1815
                     /locus_tag="P5316_14500"
     CDS             1372..1815
                     /locus_tag="P5316_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86214.1"
                     /translation="MTAFFASQEEPGRTKTVVKVVAACVMLAAAAFFALRASSEDDQL
                     DTPESAVTYICWHDNHTFKLTPAQWDKLLKQGDVQPVGKGGAAAATSSREQRGGGGGP
                     IRAVKCPKCGKFSCVPALDCPDGTQVPTITVNGQVGECPNTPGGG"
     gene            complement(2012..3205)
                     /gene="rhmD"
                     /locus_tag="P5316_14505"
     CDS             complement(2012..3205)
                     /gene="rhmD"
                     /locus_tag="P5316_14505"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HRR86215.1"
                     /translation="MKKIVDVRAYVVGGADQGADYHAQSSGHWITSTVIATPMSKYPE
                     YQKTRESFGIGVLGTTMVEVEAEDGTAGFAVTTGGVPAAYLIEHHFKRFVVGKTADDT
                     EIMFDQMYNASLFYGRKGLTINAISAVDCAVWDLRGRLRGEPVWAMIGGKVRDRQPMY
                     ATTPRPDLAKQMGFAAAKIPLPYGPAHGEEGLAENVKLAARWREACGPDFPLAYDCWM
                     ALTPEYTLRLIEALEPYRFRWIEECLPPDDYAGYRTLVERRSHPIWITSGEHEYTRWG
                     FDNLLATGVDMIQPDVTWCGGLTELLKIKAMAAARNRLVVPHGSSVYSYHFAITSDAT
                     PFAEFLMMSPKADRIVPMFSPLFVDEPVPVDGYLEVPDKPGFGVRFNPKLERQRPFVV
                     APSRG"
     gene            3436..4539
                     /gene="mutY"
                     /locus_tag="P5316_14510"
     CDS             3436..4539
                     /gene="mutY"
                     /locus_tag="P5316_14510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRR86216.1"
                     /translation="MAISRPLLRWYDANRRDLPWRRRFDDPYAQLLAEFMLQQTQVST
                     VLNYYERFIKRFPTVRSLAAAELDDVLALWSGLGYYSRARNLHAAARKIVEQYGGTVP
                     RTVDELMRLPGIGRYTAGAIASIAYDVRAPVLDGNVIRVLMRLSAMRADPKSPTARKR
                     LWDRAESLLPRKRCGDFNQAMMELGATLCLPRAPNCDVCPLKRCCLARLRCLTDQIPP
                     TARRARVVPARMVVAAIRRTGVRTSVQEWLFVQRPPSGLWAGLWELPSEPVADGESLV
                     AACKRLLAGLPISCRLSRAPKATVTRRLTHRRITFEIFSGTAGDGQSPPRPWRWISPG
                     PNRRLGTSRACEVIMELLGARRTLAKPAKASDK"
     gene            4758..5951
                     /locus_tag="P5316_14515"
     CDS             4758..5951
                     /locus_tag="P5316_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86217.1"
                     /translation="MHTMRLFGRGALIGMVVLACVLPAQGQWSRDFGPGTFILGDGTL
                     IDPSGTTGMVLYDPVYNAVGVHEVWNSVVNESLRMGFNGLHDDNDSGLMIDVDVIAGS
                     PRVVRGNISAKVLIRYSTNPLLAQTPTNSELNAGWLLRVDLVNLNGYICVMDDRGYLQ
                     LQKLVAGEVQNACPSGGYKTVAGFDVTKDWWVRFELVDNLDNTTTLRAHAWQDGTPEP
                     NDCMDWTVNGVLCVDEAPLPDGAAALLINEDDNLGGQVNYIDLDNASISTEITCIEIC
                     DNELDDDGDSLIDCADPDCDIIPPCACPDPFADADRDGDVDQADFALFQVCFTGEGDP
                     NEMFDRAACDCFDREDTDGNGQFRPSIDGDDDIDWADLEAFEACASGAGIPAAAACDD
                     APRAE"
     gene            complement(6273..7253)
                     /locus_tag="P5316_14520"
     CDS             complement(6273..7253)
                     /locus_tag="P5316_14520"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRR86218.1"
                     /translation="MIQLCMVLMTTIAAEGRLGQAPTSMPQGAQSSAPARPARTYRNP
                     IIDRIGPADPAVIRYRGKYYLYPTLDGKGYDVFASDDLVHWEQKGKCYTDPRGGAWAP
                     DVFHHIKGDGKFYLYYSVNKPGGGKLIGVAVADDPLGPFTDKGNLVNDAIDAHLFQDD
                     DGSMYLYYSAMSPGGSNIVVQPMADTLSKKGEPVTLIRPTDEWERHRAPIAEGPWMLK
                     HKGTYYLMYSGSGADGPDYGVGYATAKSPTGPFTKYAGNPIAKRGNGVFGPGHHCVIA
                     GPDGRLWMVYHQQNSEKPGWKRFLAIDPLWFDEQGVIHVKTTRGTDEPAP"
     gene            7476..8699
                     /locus_tag="P5316_14525"
     CDS             7476..8699
                     /locus_tag="P5316_14525"
                     /inference="COORDINATES: protein motif:HMM:NF037273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86219.1"
                     /translation="MNSQVSVASMSFRSRFLLSLVSIAIGTAVWLPLLHLVFRPGRDQ
                     FRSETTVAPLARQLARRHLDLWTLPGDGKADIDKMRVNNPEWDFMGRTFLVLSLANMC
                     LHEPQQQDEYLQVMDRIIEDTDRLAQAKGISYFLLDYANQSQFLQQPVRSIFVDGELA
                     MMMGARRLVAEHEPFRERMRELVHITADRMAVGPVLSCESYPNECWTFCNTVALAAIR
                     MHEVLDGEDHGQLRRDWIARAKEKLVHQQTGLLCSSYTYEGQAVDGPEGSTIWMAAHW
                     LKIVDEAFAEDQYRRAKREIAHQCLGFGWAGEWPKSWVGPMDIDSGPVIPIIDLSAGS
                     SGQAFVAAASFGDIEYYGALTTTLMFAGFPLFHDGRMQFCASNQVGDAVLLYSAVAGP
                     LWQTIRERGRRVEAP"
     gene            8696..9061
                     /locus_tag="P5316_14530"
     CDS             8696..9061
                     /locus_tag="P5316_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86220.1"
                     /translation="MMGRWRTLHRRIRAMKWFSPGSFVLCAATFVVVYLVLHLLGWRE
                     STSIFCGTLPEGRNAQVLQSFQAVMYVLFYMATAVVAPILLIAAAVFQVMAMLWIRRA
                     GEFSMKMQGPVSKDGGSAA"
     gene            complement(9335..9826)
                     /locus_tag="P5316_14535"
     CDS             complement(9335..9826)
                     /locus_tag="P5316_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86221.1"
                     /translation="MILLVVIILSTVIACWSMNIGLYAMLSRYVMVVLAVAAGIGFSG
                     PLRQSIPTDNRFLYGPCLLTVAVLMYALQRKLAQGCLDEPELALPPFINRLGGGLLGF
                     LLTMTCLSYLALVLATFPLPEQDVILPEIRQIARLAVGTARAVSLLAGTGRQITLDTV
                     LPQ"
     gene            complement(9823..11340)
                     /locus_tag="P5316_14540"
     CDS             complement(9823..11340)
                     /locus_tag="P5316_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRR86222.1"
                     /translation="MKYPPFLISPLWLTVLVGLMYLWLHCVAGLSKRLTSQNRSAPYL
                     NLLIILTGPFAWLGWWGKNHRLHTSEGWKELASNIQAFVRRGWVGRRYEDDVITVLAR
                     ADGTAAAGLSRGSGRGSEPMALTLARQIIDWAVDLRASDILMDPKPDQSYELRYRVDG
                     LLREPQRIERETALATLNCFKILADMNIAERRRAQDGSLLAYYNDREIKLRVATAGTV
                     YGEKIVIRVLDAAVGLFRLDQLGLSQTQMDRLRGQIHRAHSMLLICGPTGSGKSTTLY
                     AAINELGNTGRNIITIEDPIEYALPIASQTAINPKADITFASQLRHVLRQSPDVIMVG
                     EIRDAETARIALQASETGHLVFSTLHSNDAVTGLIRLIELGIEPHTISASVNCLMSQR
                     LVRRLCTKCRARTSISTRLKREADQRRIPLGNIYEPVGCPACDGTGYSGREGIFEMLE
                     MSPALGEQLATRPTMNALFGVARTAGMTTIRQHGIAKVLNGTTSVAEVVRVTVFR"
     gene            complement(11375..11914)
                     /locus_tag="P5316_14545"
     CDS             complement(11375..11914)
                     /locus_tag="P5316_14545"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRR86223.1"
                     /translation="MVSYSTPPEAIDERFIEILIEELGPKGPRLPAGEDDQTLPIPPK
                     PASHDDELLPLPLEPLEDTVLDHPPLPQEVPAAAPLVLRIVEPGEPDQVVKLAGNAIG
                     IGRSRINTVVVRCPRVSRKHLRVWMEAGAVWVEEMTGNHIMSVNGVRMPRARLQLNDE
                     VQVGSVKLFVESQESGESQ"
     gene            12314..>12832
                     /locus_tag="P5316_14550"
     CDS             12314..>12832
                     /locus_tag="P5316_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR86224.1"
                     /translation="MEAALPEDLSRSNADDRREAVARLAESGDYARPEVFHVLDAVAR
                     TDPVSQIRCIAIRGLARYKDDRPVGTMLAVLQAAKPGDKAVPADDDVRWEALHALAEL
                     DQRKALASAQREIARDLAIRMAEFDSSRSVRITATEMLGCFKDRAVLAPLIRLLRDRD
                     FAIAERAELSLIA"
BASE COUNT         2390 a         3769 c         4183 g         2490 t
ORIGIN      
        1 ttttttacga acaaagcgag aaaacctcgc cggagtatgc ttgatcaggg actagccgtt
       61 tgctgggccg ccgccggcag atagaggccg gcgagcaagg cgaccccaac aatgacacct
      121 gttcttgaaa tcgacttcat gtgacactca cccgtcggaa tcagcgctcg gcgtttgttt
      181 gacgtgggcg gtggatcgcc ccctatcaga ccccgcgcca ggccctgggc aagtcggtca
      241 ttatacggcg ttcccggcag ccaagcgaac cactgagacc gtcgtgcgtt gcggcgaggc
      301 tctctcaaga gcggttgggc aatccccggc cgtgcaatcg atgcggtacc gaaaatgatg
      361 aatctggccg gttccatccg attcaatcgg gtgatagaat gctaaaaggt acgcccggcc
      421 cgaaaggcgc cggtcacatg gcgctgtcga tcaacacgtc gggcaaaagg tgaaggggat
      481 gacccggccc taatgtgaga ggtaggtacc agcatgcata ctggatccgt cagtcatgga
      541 atctcttgtg ttcggcgttg gtccggcggt tttactctca ttgaagtgct tgtcgtggtg
      601 gccatcatgg ctctgctcat ttcgattctc ttgccctcgc tgtcggaggc gcgcgaacaa
      661 gcgaaacgcg ccgcctgttt gtcaaacctg aaaggcctcg gcacggcggt cacggcgtac
      721 atcctcaacg agaaagagcg tttttgctgg atgcccaaga cctggctctt cggcggcaga
      781 aagggcaacg gcgaggtcac gtggggaggc tgggacgtac ctgctgagcg tcgcccatta
      841 aacagatacg tctaccaagg aaagatgcgt aacagcatgt ctctggaggc ttaccgctgt
      901 ccgtcggact tcggtgttcg tctgaacaac gagccggaca gccaagccac caagagcccc
      961 ggctatgacg tgctcggaac cagctaccaa tccaaccaca actgggcatc gtatgcggac
     1021 agctccccgg acatgggtcc ggggggtgaa ggctatgcgg gaacggccct cgatcagaga
     1081 atcgattatc tcgaggacaa catcgtccgg atgatgctga ggaaggcccc gacgcgcttc
     1141 atcgtgctgt acgaggaccc ggccgactgg gcgctcaata cggcggacaa gttcattccg
     1201 aactacaagg tgacctcctg gcacaaaaag ccgaactacc actgcatggg ctttctcgac
     1261 gggcacgccc agtatctcta tgttgatttc aagaagaaca gtcgcttgcg tcatatcagc
     1321 ggtacggtcg actggttggc ccggcaggac tatcgggagc aatgaacgca catgacagct
     1381 ttcttcgcat ctcaagagga acccggacgt acaaagaccg tcgtgaaagt agtcgcggcg
     1441 tgcgtgatgc tggcggcggc ggcgttcttc gccctgcgag catcatccga agatgaccaa
     1501 ctcgacacgc cggagtcggc cgtcacttac atttgctggc acgacaacca caccttcaag
     1561 ttgacgcccg cccaatggga caaactgctt aaacagggcg acgttcaacc ggttggcaag
     1621 gggggagcgg cagccgcaac ctcgagtcga gaacagcggg gcggcggagg cggaccgatc
     1681 cgagcggtca agtgcccgaa atgcgggaag ttcagttgcg tgcctgccct cgactgtccc
     1741 gacggcaccc aggtgcccac catcacggtg aatggacaag tgggagaatg cccgaacacc
     1801 cccggcggcg ggtaggatgt ccggttcttt ggtcctcatg gtcgcagacg cccgcacgct
     1861 tcttcgcgtg ttcgaggaca attcgagcat gagggccggc atgtacgtcc cgcggccgac
     1921 gaggtgaagg gtgcctcatc gcacaggggc gggagacagc catttgcagc gtaccgtctc
     1981 cggtctgcgc ccacggattc atcaaggagc ctcacccacg agatggcgcg accacaaacg
     2041 gcctttgtcg ctcgagcttc ggattgaacc gcaccccgaa tccgggtttg tcgggtactt
     2101 cgagatatcc gtcgaccgga acgggctcat cgacgaacaa cggcgaaaac atcgggacga
     2161 tccggtcggc cttcggggac atcatcagga actcggcaaa cggcgtggcg tcgctggtga
     2221 tcgcgaagtg atagctgtag accgacgagc cgtgcggaac cacaagacga ttgcgggccg
     2281 cggccatcgc cttgatcttg agcagttcgg tcaacccgcc gcaccaggtc acgtccggct
     2341 gaatcatgtc cacaccggtg gccagcaggt tgtcgaagcc ccaccgggtg tattcatgct
     2401 cgcctgaggt gatccagatg gggtggctgc ggcgctcaac gagggtccga tacccggcgt
     2461 aatcatccgg cggcaggcat tcctcgatcc accgaaaccg gtacggttcc agggcctcga
     2521 tcagccgtag cgtgtactcc ggcgtcaggg ccatccagca gtcgtacgcc agcgggaaat
     2581 ccggcccgca cgcctcgcgc cagcgggctg cgagtttcac attctcggcc agcccctctt
     2641 caccgtgggc cggcccatat ggcagcggaa tcttggcggc cgcaaagccc atctgtttgg
     2701 ccagatccgg ccgcggcgtg gttgcgtaca tcggctggcg atctcgcacc ttcccgccga
     2761 tcattgccca gaccggctca ccgcgcagcc gcccacgcag gtcccacacc gcacagtcca
     2821 ccgccgaaat cgcgttgatc gtcagcccct tgcgcccgta gaacagtgat gcgttgtaca
     2881 tttggtcgaa catgatttcg gtgtcgtcgg ccgtcttgcc gaccacgaat cgcttgaaat
     2941 gatgctcgat caggtaagcc gcgggcaccc cgccggtcgt caccgcgaag cccgccgtgc
     3001 catcctctgc ctcgacctcc accatcgtcg tgcccagcac gccgatcccg aacgactcgc
     3061 gagtcttctg gtactcggga tacttgctca tcggcgtggc gatcaccgtg ctggtgatcc
     3121 agtgcccgct cgactgggca tgataatccg ccccctggtc cgccccgccc accacatatg
     3181 cccgcacgtc aacgatcttc ttcattacag tccctcgacg gcattgcccg aacaggttag
     3241 gccctgcaaa agggccacgc aagcacaccc gcgctggctt gatgcaggcc ttcgagtgat
     3301 gacaggcaac tgagccgtga ctatcattcc tcttcggcga tcggagggtg gaccgtcatg
     3361 ccccagaaag cagccgcgag agactcgcat acaaagcgtt gttcgcgtga ttctggccct
     3421 cggccctcgc ccctcgtggc catctctcgc cctctgctgc gctggtacga tgcaaaccgc
     3481 cgcgacctgc cctggcgacg acgtttcgat gacccgtacg cccagctcct ggccgagttc
     3541 atgctccagc agacccaggt cagcaccgtt ctcaactact atgagcgttt catcaagcgc
     3601 ttccccactg tccggtcctt ggccgccgcc gagcttgacg acgtgctcgc gctgtggtcg
     3661 ggcctaggct actatagccg tgcccgcaat ctgcacgccg ccgcccgcaa aatcgtcgag
     3721 caatatggcg gcaccgtgcc gcggacggtg gatgagctca tgcgcctgcc cggcatcggc
     3781 cgctacaccg ccggggccat cgcgtcgatc gcctacgacg ttcgcgcccc cgtgctggac
     3841 ggcaacgtga tccgcgttct catgcgtttg tcggccatga gggccgatcc gaaatcaccc
     3901 actgcaagaa agaggctttg ggatcgcgcc gaaagcctgc tgccgcgcaa gcgttgcggt
     3961 gatttcaatc aggcgatgat ggaactcggg gccacgctgt gcctgccgcg cgcgccgaac
     4021 tgcgacgtat gtcctctgaa gcgatgttgc ctggcccgtc tgcggtgcct gaccgatcag
     4081 attccaccga ccgcgaggcg agctcgcgtg gtgccggccc gcatggtagt cgcggcgatc
     4141 cgcaggacgg gtgttcgaac gtccgtgcaa gagtggctct tcgttcaacg tccgccgtcc
     4201 ggcctctggg ccggtctgtg ggaactgccc agtgagccgg tcgctgatgg agaatcgctc
     4261 gtcgccgcgt gcaaaaggtt gctggccggt ttgccgattt catgccgatt gagccgagcc
     4321 cccaaagcca ctgtgacccg ccgactcacc caccgtcgca tcaccttcga gatctttagt
     4381 ggcacggccg gcgatgggca gagcccgccc cggccatggc gatggatctc gccaggtccg
     4441 aaccgccgac tgggtaccag ccgggcctgt gaagtgatta tggaactgct gggagcccgc
     4501 cgaacgctcg ccaagccggc aaaggcgtcg gacaagtaac aacagcatta ttacagtgaa
     4561 aatgcgtaat tgccttcgct ttcgcggcgc cgagcgcttt gccccgaaac ttcaaactca
     4621 ttgcggaatt tgaaaaaaag gacttgaacg aatgagccat gtgggggtat ggtaaagatg
     4681 gtgaccgttc atgtcgtacg gcgccgtgga tgttcaaggc agtcaactca tgagtcattt
     4741 caggaaaggg attgaacatg cataccatgc gtcttttcgg ccgcggagca ctgattggga
     4801 tggtggtcct ggcctgcgtc ctgcccgcgc agggccagtg gtcgcgagat ttcggtccgg
     4861 gcacgttcat tctgggggac ggcacgctga tcgaccccag cggcaccacg ggcatggttc
     4921 tctacgatcc ggtctacaac gccgtgggtg ttcacgaagt ctggaattca gtcgtcaacg
     4981 agtctctccg aatgggtttc aacggactgc acgatgataa tgactccggt ctgatgatcg
     5041 atgtcgatgt gattgcagga tcgccgcgcg tcgtcagagg caacatctcc gcgaaagtgc
     5101 tgattcgtta ttccaccaac ccgctcctgg cccagactcc gaccaactcg gaattgaacg
     5161 ccggctggct gctgcgcgtg gatctggtga acctgaacgg ctacatctgc gtcatggacg
     5221 accgtggcta tcttcagctt cagaaactgg tagccggcga ggtccagaac gcatgccctt
     5281 cgggtggata caagactgtc gccggcttcg acgtgaccaa ggattggtgg gtgcgattcg
     5341 aattagtgga caaccttgat aacaccacca ccctccgagc ccacgcctgg caggacggga
     5401 ctccggaacc caatgactgc atggattgga cggtgaacgg ggtgctatgc gtggacgagg
     5461 ccccgttgcc tgacggggct gcggccctgc tgatcaatga ggatgataat ctcggggggc
     5521 aggtcaacta catcgatctc gacaacgctt ccatcagcac tgagatcacc tgcatcgaga
     5581 tatgcgacaa cgagctcgac gacgacgggg atagcctgat cgactgcgcc gatccggact
     5641 gtgacattat cccgccctgc gcctgcccgg acccgttcgc cgacgccgac agggacggtg
     5701 acgtcgacca ggccgacttc gctcttttcc aagtctgctt cacgggggaa ggcgacccga
     5761 atgagatgtt cgaccgggca gcatgcgatt gctttgatcg cgaggacacg gatggcaacg
     5821 gccagtttcg gccctccatt gacggcgatg acgatatcga ctgggcggac ctggaagcat
     5881 tcgaggcctg cgcatcggga gcggggatac cggcggccgc cgcctgcgac gacgcgcccc
     5941 gagccgaata gtcggtctca tctgcaggct ccccggtggc gagggtcgcg cggccgtggg
     6001 tccgcgcttc gagacaggcc gccgatgggt tcatggactt gcggcgggag cctggcgtag
     6061 gaggacctgg agcccttgcg gacaagccgg cggcacggga acccggagcg attcacgcat
     6121 caatgcggca acgacgtcat ccatcaggac gataacagca tgttcgaaca gggcgtcagc
     6181 ggctcgggca ttccggccga cccgcgggtt gctgaaacat tgcttccgtt gccgattccg
     6241 tctcttgcgc cttggggatg gggtaccctg tcctacggag cgggctcgtc ggttccacgg
     6301 gtggtcttca cgtgaatcac gccctgctcg tcgaaccaca gtgggtcgat ggccaggaaa
     6361 cgtttccatc caggtttttc gctgttttgc tggtgataga ccatccacag acggccgtcc
     6421 gggccggcga tgacgcaatg atggcccggg ccgaagacgc cgttgccgcg tttggcgatc
     6481 gggttgcccg cgtacttggt gaacggcccg gtgggcgact tggcggtggc gtatccgacg
     6541 ccgtagtccg gaccatcggc gccgctgccg gagtacatca ggtagtacgt gcccttgtgc
     6601 ttgagcatcc aggggccttc ggcgatgggt gcgcggtgac gttcccactc atcggtcggc
     6661 cggatgagcg tgacgggttc gcccttcttg ctcagcgtgt ccgccatggg ctgaacgacg
     6721 atgttgctgc cgccggggct catggccgag tagtacaggt acatcgatcc gtcatcatcc
     6781 tgaaacagat gggcgtcgat ggcatcgttg accaggttgc ccttgtcggt gaagggtccg
     6841 agcgggtcgt cggccacggc cactccgatc agcttgcccc cgccgggttt gttcacgctg
     6901 tagtagagat agaacttgcc gtcgccttta atgtgatgga agacatcagg agcccacgcg
     6961 cctccgcgcg ggtcggtgta gcacttgccc ttctgttccc agtgcaccag gtcgtcggat
     7021 gcgaacacat cgtaaccctt gccgtcgagc gtcgggtaga ggtagtactt gccgcggtag
     7081 cggatgaccg cgggatcggc gggtccgatg cggtcgatga tcgggtttcg gtatgttcgt
     7141 gccggccgag cgggggctga cgattgagcc ccttggggca ttgacgttgg ggcctggccc
     7201 aagcggcctt cagccgcgat tgtcgtcatg agaaccatgc atagttgtat cattgtttgc
     7261 tcccggttgc aggaaggatc ggcggtcaaa acagggtccg agcccggtct tttgggaccg
     7321 ggcgatgccg acttgggaat ttccccgatg ggcacgtttt tttcaagccg cacagcccgg
     7381 ttctgcaaac ccgatggtcc ggcggggtcg gttcatgtat agtgcaatgt ggatggacga
     7441 gcgtgcagtg cgggatgtga gtcggggaga ccggggtgaa ttcgcaggtg tcagtagcaa
     7501 gcatgtcctt tcgctccagg ttcctgttga gcctggtttc gattgccatc ggaacggccg
     7561 tctggctgcc tctgctgcat cttgtcttcc ggcccggtcg tgaccagttc cgcagcgaaa
     7621 cgaccgttgc gccgttggcc cgccaattgg ctcggcggca cttggatctc tggacactgc
     7681 cgggggacgg caaggccgat attgacaaga tgcgggtcaa caacccggaa tgggatttca
     7741 tgggccggac gttcctggtt ttgtcactgg ccaacatgtg tctccacgag ccgcagcagc
     7801 aggatgagta cctccaggtg atggacagga tcattgagga cacggatcgt ctcgcccagg
     7861 cgaaagggat cagctacttc ctgctcgact acgccaacca gtcgcagttt ctgcagcagc
     7921 cggtgcgcag catatttgtc gacggtgagc tggcgatgat gatgggggcc cggcgactgg
     7981 tggccgagca cgagccattt cgagaacgca tgcgggaact cgtgcacatc accgccgacc
     8041 gtatggccgt cgggccggtt ctctcgtgcg agagttatcc gaacgagtgc tggacgttct
     8101 gcaacaccgt cgccctggcg gcgatcagga tgcacgaggt gctcgacggc gaagatcatg
     8161 ggcagcttcg gcgggattgg atcgcgaggg cgaaggagaa gctggtgcac caacaaacgg
     8221 gtctgctttg ttcatcctac acgtacgagg ggcaggcggt ggacgggccg gaaggctcga
     8281 cgatctggat ggccgctcat tggctgaaga tcgtggatga ggcattcgcg gaggaccagt
     8341 atcgtcgggc caagcgggag attgcgcatc aatgcctggg tttcgggtgg gccggcgagt
     8401 ggcccaagtc ctgggtcggg ccgatggaca ttgattcggg gccggtgatt ccgattatcg
     8461 acttgagcgc gggctcgagc gggcaggcat ttgtcgccgc ggcatcgttc ggggacatcg
     8521 agtactacgg ggcgctgacg acgacgctga tgttcgcggg gttcccgttg tttcacgacg
     8581 gccggatgca gttctgtgcc agcaaccagg ttggtgacgc ggtactgctg tattccgccg
     8641 tcgccgggcc gctttggcag accatccgcg agcgaggacg aagggtggag gcaccatgat
     8701 gggcaggtgg cgaactctgc accggcggat cagggccatg aaatggttct cacccggttc
     8761 atttgtgttg tgtgccgcca cctttgtggt tgtctatctg gtcctgcacc tgctcggatg
     8821 gcgtgagagc acaagcatct tctgcggaac cctgcccgag ggccgcaatg cccaggttct
     8881 ccaatccttc caggccgtga tgtacgtgct gttctacatg gccacggcgg tcgtcgcacc
     8941 gatcctgctg attgcggccg ctgtattcca ggtgatggcg atgctgtgga taagaagggc
     9001 cggcgagttc tcgatgaaga tgcagggacc ggtttcgaaa gacggaggct ccgcggcatg
     9061 aggctgtacc ggcgatcatc gaaccgcaat ccgattctgc gggatgccgg cgcagggggt
     9121 cgactgcggc ggttcatctg atccgtgatc atcagaagaa gacggtagag gggtcagcac
     9181 gcgtatcgct tctgaatatg acgtgtttga gccgcagagg accaccgtca ggatcggtcg
     9241 aaacaggcac cgtgtttgcc cgggagcgca tggtcaagac aacgccaagc gagggcggca
     9301 gtcagtgcgg ggggggtcgc cttcctttgt acctctactg cggcaggacg gtgtccaggg
     9361 tgatctgccg gccggtgccg gccaggagac ttaccgcccg tgcggttccg acggcgaggc
     9421 gagcgatctg cctgatttca ggaagtatca catcctgctc gggcaggggg aaagtggcga
     9481 gaacgagggc cagatagctc aggcaggtca tggtcagcag gaaacccagc aggcctccgc
     9541 ccagccgatt gatgaacggc ggcaaggcga gctcaggctc atcgaggcag ccttgggcca
     9601 gtttgcgctg cagcgcgtac atgagcacgg cgacggtgag caggcacgga ccgtagagaa
     9661 agcggttgtc cgtcggaatg gactgtcgca aggggccgga aaagccgatc ccggccgcga
     9721 cagccaggac gaccatgaca tagcggctca gcatggcgta gagacctatg ttcatgctcc
     9781 agcaagcgat gacggtcgac agtatgatca cgacaagaag aatcatcgaa acaccgttac
     9841 gcgaacaacc tcggccacgc tggtcgtgcc gttcagcacc ttggcgatgc cgtgctgcct
     9901 gatcgtggtc atgccggcgg ttcgtgcgac gccgaaaagg gcgttcatcg tgggccgggt
     9961 agccagttgc tctccgaggg ccggcgacat ttcgagcatc tcgaaaatgc cctcgcggcc
    10021 gctgtagccg gttccgtcac acgccgggca gccgacgggc tcgtagatgt tgcccagcgg
    10081 gatacgtcgc tggtcggcct cccgcttgag acgagtggaa atcgaggttc gggcccgaca
    10141 tttggtgcaa agcctgcgaa ccaggcgttg agacatcagg caattcacgg atgcggagat
    10201 ggtatgcggc tcgatgccca gttcaatcag acggatgagc ccggtcacgg cgtcattcga
    10261 atggagagtc gagaacacga gatgtccggt ttcggaggcc tggagtgcta tcctggcggt
    10321 ctcggcgtcg cggatctcgc cgaccatgat aacgtcgggg gattgtctca agacgtggcg
    10381 aagctggctg gcaaaggtaa tgtcggcctt ggggttgatg gccgtctggc tggcgatggg
    10441 aagcgcgtat tcgatgggat cctcgatggt gatgatgttg cggccggtgt tgccgagttc
    10501 gttgatggcg gcgtaaaggg tggtgctttt gccgctgccg gtggggccgc agatgaggag
    10561 catgctgtgt gcccggtgga tctggccgcg gagccgatcc atctgcgtct gggagaggcc
    10621 gagctggtca aggcgaaaca ggccgacggc agcgtcgaga acgcggatga cgattttctc
    10681 gccgtacacg gttcccgcgg tggcgacgcg gagcttgatc tcgcgatcgt tgtagtaagc
    10741 cagcagtgag ccgtcctgtg cgcggcggcg ttcggcgata ttcatgtccg ccagaatctt
    10801 aaagcagttc agcgtagcca gggccgtttc gcgctcgatc cgctggggct cgcgaaggag
    10861 gccgtcgacg cgatagcgga gctcataact ctgatcgggc ttggggtcca tgaggatgtc
    10921 ggatgcgcgc aagtcaacgg cccagtcgat gatctgccga gccaaggtca gggccatcgg
    10981 ctcgctgccg cgtcctgatc cgcgggacag gccggctgcc gccgtcccgt cggcgcgcgc
    11041 cagcacggta atgacatcgt cctcatacct gcggccgacc cagccgcgcc ggacaaatgc
    11101 ctgaatgttt gaggcaagtt ccttccaacc ctcagaggta tgcagccgat ggtttttgcc
    11161 ccaccaaccg agccaggcaa acgggccggt caagatgatg agcaggttca ggtaaggtgc
    11221 ggagcggttt tgcgaggtca gccgcttgct gaggccggcg acgcagtgca gccagaggta
    11281 catcaggccg accagcacgg tgagccagag cggacttatg aggaaggggg ggtacttcat
    11341 gccttgctcg ttgtcgggcc ggcgggtagc cggctcattg tgattcgccg ctttcctggg
    11401 actccacgaa taacttgacg ctgcctacct gcacttcgtc gttcagttgc agccgcgcgc
    11461 ggggcattct gaccccgtta acgctcatta tgtgattgcc ggtcatttcc tcgacccaga
    11521 cggctcccgc ctccatccat acgcgaaggt gtttgcggct gacgcgtggg cagcgaacga
    11581 cgacggtatt gattcgggaa cggccaatgc cgattgcgtt gccggcgagc ttaactactt
    11641 gatcgggttc gcccggctcg acgatccgca gcaccagcgg agcagccgca gggacctctt
    11701 gaggcagggg gggatggtcc aacacggtgt cctcgagggg ttcgagtggc aaaggcaaga
    11761 gctcgtcgtc gtgcgaggcc ggtttcggag gtattgggag ggtctgatca tcctcgccgg
    11821 cggggagccg tggtcccttg gggccgagtt cctcgatcag gatctcgatg aaccgttcat
    11881 cgattgcttc gggaggcgtc gagtaggaga ccatcgcagc accaccatgc acaagcgaat
    11941 aggtcgacgt cagaacagct taccacaagt gacagcggta tgggaaggtc cgccgcaggt
    12001 atcttgtgga cgcagccctt cgtcggtaga ctccgtcggg aacgccggcg ggccaagtga
    12061 gcatcgccgg accggcggtc ttggcgcggc gatctcattg cggagtggtc ggcccatgaa
    12121 ctcaggatcc atgtcgtcac ggtatggttt gggaagtaaa gatggggccc cgagggcgcg
    12181 gtgtgccggt cggctgtttg tgaagcgggc tgggcgagcg ttcgggattg ttgtgctcgg
    12241 ggtttgcctg gcttcggtcg gttgttcgcg gcaggccaag cgtcaatgga agtaccgctc
    12301 tctggagacg gccatggaag cggcgttgcc tgaggacctg tccaggagca acgcggatga
    12361 tcgccgggag gcggtggccc ggctggccga aagcggggac tatgcccgcc cggaggtctt
    12421 ccatgtactc gacgcggtcg cccgcacgga tccggtcagc cagattcggt gcatcgccat
    12481 tcgcggcttg gctcgctaca aagacgatcg gccggtcgga acgatgctcg ccgttttgca
    12541 ggcggcgaag cccggtgaca aggccgttcc ggctgacgat gacgtgcggt gggaggcgtt
    12601 gcatgccctt gccgaactgg atcagcgaaa agcgctcgcg agtgcccagc gtgaaatcgc
    12661 ccgagacctg gcgattcgga tggccgagtt cgattcgtcg cgcagcgtgc gtatcacggc
    12721 gacggaaatg ctcggttgct tcaaggaccg ggccgtgctg gcgccgttga tccgcctgct
    12781 gcgtgaccgg gactttgcca tcgccgagcg ggccgagttg tccttgatcg cc
//
LOCUS       DAOTPW010000164        12690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4756_length_12690_cov_5.882786, whole genome shotgun sequence.
ACCESSION   DAOTPW010000164 DAOTPW010000000
VERSION     DAOTPW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4756_length_12690_cov_5.882786"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            756..1865
                     /locus_tag="P5316_14555"
     CDS             756..1865
                     /locus_tag="P5316_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86225.1"
                     /translation="MPRSVVYGLFVVVLFTCLTGQNCLGPNPGGQADTSDNSLDSSDG
                     VTDGQADGGNVDDDQDQGENDNGDTEAGDFTPASGAAVGPSGYGPWNNRLRVATSNDG
                     MNWTRTGVTLADQADVPCCLVVDGTLWVFFVIWQDATGSPDLTNTTVAACSRDLVRWV
                     YRKLDFAGLPQGYTPTPVDPTVVAFDGGYRMYFTLGPQPASETEKPHTFSATSTDLIH
                     WIYEGERYDRGNKEVLDPNLLWIDDHYEFFAGGAAEINHHATSADGLSFTVLADLMPL
                     ADSGFGVVMCNGMKVGGTYKYYGFLSVPAGSSTPVDIRSLTYDGGRWRVDDGARLSLD
                     SSTGQESAYVKDPGVSVHPAGEAAGYVMIYVTAIP"
     gene            complement(1999..3528)
                     /locus_tag="P5316_14560"
     CDS             complement(1999..3528)
                     /locus_tag="P5316_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86226.1"
                     /translation="MLRLHSLQTPPDDGGILLEPPPEHWRALLEQNVRLLSKIGGALA
                     GTAITEARQQVRSQLLGVAGNARIVAAGHQPEFVHPGVWAKHVAVRAFADTAGMSGID
                     LVVDNDAPRSAGLRVPVRGGDGLLAVREVPFTTAPPGSAYEGRTPLTAMRIAEIRQEL
                     AVVLGESLDDTLMPAYLDGLAEWAGRGDAVDQHLAGRLRIDRELGADLPEHRVSKVVG
                     GPFVADLLLNADRFADAYNKSLAEYRKDLAIRSADRPLPDLARVSGRIETALWAYQPL
                     QRRRRLWIEPKGDALRCYAEENAIGELSRADLTRDFAAAAESLRPWVIRPRALTLTLW
                     ARLLACDLFVHGIGGAKYDRITDGIFRRYYACEPPAYVCVSATLRSPWPNLPATVDDL
                     RAAGRRVRDWWFNPQRYCPDLPASLLRERERLIRRSDQLREARGPRLERREVFNAIRA
                     VNRRMAESHGEIARALADRRDFLARQVESNRIAAGREHFYALQTRERLDMLADRITRI
                     L"
     gene            complement(3531..4325)
                     /locus_tag="P5316_14565"
     CDS             complement(3531..4325)
                     /locus_tag="P5316_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR86227.1"
                     /translation="MRYLVAIPVYNEAKTVIGVLRQVRQYAPDGDILVVDDGSTDQTP
                     RLLQAEPGISVIRHIDNRGYGQSLIDAFRFAVSRDYDWLITMDCDEQHEPAWIPLFLQ
                     MARQDDADIISGSRYLQPLPGNSRPPADRRAINQKMTDMLNEILRLGITDAFCGFKAY
                     RVSALARLNITVPGYAMPIQFWVQAARRGLRIREIPVRLIYNDPNRYFGGSLDDPDAR
                     LLYYYDVLVHALSEQIDRPVRAEIDPESAAPRQACGLASGLAQPVE"
     gene            complement(4499..5764)
                     /locus_tag="P5316_14570"
     CDS             complement(4499..5764)
                     /locus_tag="P5316_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86228.1"
                     /translation="MLSEARLAQNQTPKDGVSYILAPSVNDEHGEVRVPPGGTIALFF
                     ALLVHGAFAQGLSAQQSRPAHEAPRPIVGAIRWDAWFGNHGVVGEAVHRSLGPSKWHN
                     RLPFYAKVNGDEAIEIDGTPQAVMDREIEYAAAAGLDYWAFVTYPADDPLSLALRRYL
                     SSSVRSKIGFCLITECGRWPHQAFVDRVVSLMQEPGYQRVLDGRPLLYLGFINEQWLE
                     KLGGVEGFRRVLDTFRATVTARGLPRPYIVIMDFSPQQGKKWAEALGCDAISSYVAGL
                     GKGVIPYARMVENLEHFWEECRRTGAQVVPIVVTGWDPRPRVEKPMPWGSPYGSHDGE
                     VDRSEPATPAAIADHLRRGLRWLETHRHAAPAQAAIIYAWNEFDEGGWLAPTLSEGTA
                     RLDAVAGVLRPGATSRPSAVSHLATSTQP"
     gene            6135..6851
                     /gene="grpE"
                     /locus_tag="P5316_14575"
     CDS             6135..6851
                     /gene="grpE"
                     /locus_tag="P5316_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRR86229.1"
                     /translation="MAGKTSPPRKSSSVTPRAARKKKGLTKPSRSTATKPATQAKGPA
                     AGGRRPQAADDRSPAGLLAQAEADLTALLDSLNSHMATAVNAITELAVAQQGQHQPII
                     RSRPIDRATAMFQRLVAEVLDDKLAEVLPTLVALRSEMNQRARLVGSSASTESTPPTD
                     GDFFERGTAMLDQVLNTLEVRSFEPRIGDPFDPLIHLAVGQTSRNDLAEGVLAEILQP
                     GFRTARGKVIVPARVKVNRR"
     gene            6856..8469
                     /locus_tag="P5316_14580"
     CDS             6856..8469
                     /locus_tag="P5316_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRR86230.1"
                     /translation="MARLGIDFGTTNTVAVIHDRGVFSVVLHRADTGAGTIVQDVFPS
                     AILIDKRSGKHWFGLEADRLFGQRGPGPDHVFLPSLKRHLWSYAEGHSVSSGHEPRPR
                     ARDVAAGLEQFDIGKLLTGFLQSLAESIRASGMIDPGEPLETVITWPANANGAQRYIT
                     RKCFREAGFDVLSTLNEPTASAIELADCITAGRGAGKDDREPSAVAVFDLGGGTFDAS
                     VVWIEGDEFEVLASGGIEDLGGDDFDRALLDMFLERFKLRDEALSPLTRHALLRQARS
                     QKETIAGGVVRSLFLNPLDFGLDCPPVSIAVEAYFERIRPMLKPAIAMLKKVIVSAAA
                     KERRVRTGSSLTVYLVGGSSKLPLVAHMVSDAFPECRVILTDKPFTSVAMGAAICATD
                     RVTYRDVFARHFGLLRLKDNGQSEVFDIIFPAGTPIPRKGEKPLERCAWYHPRHNIGH
                     LRYLECTSVGANGLPDGNVRHWSDVFFPYDPLLPIDHRASVADVVATNQFADMPACEV
                     YRCDSDGVITVELRRPTKNDSRIHEIYRD"
     gene            8631..9659
                     /locus_tag="P5316_14585"
     CDS             8631..9659
                     /locus_tag="P5316_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86231.1"
                     /translation="MHSYYLSWRWLSAWGLALASVVGCAPKDTPFAAIRAGIRCERGQ
                     KALEQEDLDAALKEFQRAVELNPKLAMAHSNLGKVQKAKGNLAAAAAAFANAVKLNPK
                     SFDDAFSLADVYHKMSKLAAAIQAYLHACELQPNNFQARLNLGVCYHQAAELDNAIDC
                     YTKAIEIDPDHAAAYTNLGAAYDAQGKYYEAIHAYNKSLERDGEQPMVLVNLAGTMMK
                     QERFAAAQRALERAIELDPELAMAYERLGYCLFRMKRYDEALTRYEWAETLDPNRAET
                     HAGLGVVRMAMFLQDRSQTEFRQAAIEHWHRSLEINPDQPKIRNLISKYRVPADQVSA
                     VLMESPPK"
     gene            9656..10723
                     /locus_tag="P5316_14590"
     CDS             9656..10723
                     /locus_tag="P5316_14590"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRR86232.1"
                     /translation="MKNRQNRAIVQCVLSCALMVFAAGCGPHKAAVHRSWAERSQTVI
                     RQRLAPTPVVPTMAASSAHPVKAERTVGGKSTIWPEELAAFHLSAETREQAGDAETRQ
                     AEESSSKQDVEYEGKWRKPLPGFGETLKRDLLELPSALWDDTKRVYTNPWNLVFLLGA
                     GGASLALRPEVDDDIEDYYDRHHTFKEDWRDAFGAAGNPIVHFGIAGAWYLTGQLAQD
                     AKTYDVGARLFRALTINGVSTLLLKTAACTESPNGEDWAWPSGHVSSAMVVATVMNDA
                     YGPLAGVPLFGLTGLVAVERLDSGEHHFSDVVFGAALGWVVAETVMKDEPPKIAGGHI
                     VPYADPVGRNAGVAWVKTLGE"
     gene            complement(10891..12186)
                     /locus_tag="P5316_14595"
     CDS             complement(10891..12186)
                     /locus_tag="P5316_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRR86233.1"
                     /translation="MIRVSVENLEPGMVLAASIPHARHPRRALLAAGYVLEPKTIAKL
                     REYGIKGVWINHPGFDFLDEQVASGVPESRARLYHDIKGNFTALAKQTAGAFDLQFYR
                     ELISRLIEELVKNQDHAVWAERILEEPEELFSHSANVAYLVMILGMNLRNYISHERKY
                     IDRRLAQDLTNLGVGAMLHDVGKIKLPPELRRSHVLDHHEDEAAYRSHAEQGYQLLRG
                     RLDSTASGVILHHHQRYDGNGFPEPISTHKERHPEPLRGRRIHVFARIVAVANVLDGM
                     MAICDRKGQPKVAALAALQAAEIRKMFDPVIFAAAVRTIPPFPVGTCVTLSDGREAVV
                     VDLNAKRPCQPVVQVLKAGAQVEDPSGERIDLVKSGAPSITHENRRPVAQYLYKPPEV
                     ITRHIAASYQDEAERSSAACSDLLETGAPSGAEQTAHPE"
     gene            complement(12403..>12690)
                     /locus_tag="P5316_14600"
     CDS             complement(12403..>12690)
                     /locus_tag="P5316_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRR86234.1"
                     /translation="PVITVEDHSVVGGFGAAVLETAQDLRLPTDHLVRLGIPSDRFIS
                     HGSRKSQLAECGIDAAGIAEAVHRELARCRPSTKAVRGFVPSSQPSHITKP"
BASE COUNT         2326 a         4079 c         3955 g         2330 t
ORIGIN      
        1 acaagaacac tctcgaaaac tcccggcatg tgggatcgcc gacgccggaa tcggttgggt
       61 ttcaaagaga tcgccgctat ccagatcacc cgcccacata ccgcttgtca ggcttggccc
      121 acaggggccc cacgcccatt ctacggcgcc cccgactgtc cgtacgctgg gacgcgggac
      181 gtgcccaggc cgctgcgaca tgcgggttaa acactgtgtt cccgcatgac ccgaagggca
      241 agccggggtc gcttgtatcg cacggatcga tttcgtgcag tgccgactga cgcgatcccg
      301 attatcctcc accacatctt ggacgcgccc gaaccgagac cgaaaagcca gagcgggtcg
      361 ggtggcacca agttggccgc gcgggagaca agccccagcc cccgaaattg ttcgcgcagc
      421 agtcgcggcg ggcggctgtt gatcacaagt cgggcgaggc acgggcgtgc ggtgagtcga
      481 gaccgttcca ggtcaaagct tggcgatatc ctgggcaacg tgggggcgag agaagccagg
      541 tgactgctgg aagtcatcca tggaaggcat ctccaacacc gtccagtggt gcgttgactc
      601 tgtcgcaccc cgtgcggcga acgctcaagc tcctcctcac caagggcgat atagccgata
      661 ggtgcttcct gtgtctgtgc ttgtgggcga aagggcatcg gcgccgtcgc tcatgccgaa
      721 tgccacaggg tcatgctgcc gcacggagaa atcacatgcc gcgttcagtt gtctacggac
      781 tcttcgttgt ggttttattt acatgcctca cgggccagaa ctgcctcggc ccaaacccgg
      841 gcggccaggc cgacacatcc gataactcgc tcgattcttc cgatggcgtc actgacgggc
      901 aggccgacgg cgggaacgtc gacgacgatc aggaccaggg cgaaaacgac aacggtgata
      961 cagaagccgg cgatttcacc cccgccagcg gcgccgccgt cggcccgagc ggctacggtc
     1021 cctggaacaa ccgccttcgc gtcgccactt cgaacgacgg catgaattgg acaagaaccg
     1081 gcgtcacgct ggcggaccag gcggatgtgc cctgttgcct ggtcgtcgac ggaacgttgt
     1141 gggtgttctt cgtgatctgg caagacgcca ccggctcacc tgatctgacc aataccaccg
     1201 ttgcggcctg ctccagggat ttggtccgtt gggtatacag gaaactcgat ttcgcgggtt
     1261 tgccgcaggg ctataccccg acccccgtcg atcccaccgt tgtcgccttc gacggcgggt
     1321 atcggatgta cttcacgctc gggccgcagc cggcgagtga aaccgagaaa ccacatacct
     1381 tctcggccac atccaccgac ttgatccact ggatttatga aggcgaacgc tacgaccgag
     1441 gcaacaagga agtcctggat cccaaccttc tctggatcga cgatcactac gaattcttcg
     1501 ccggtggcgc ggccgagatc aaccatcatg ccacttccgc tgacggattg agtttcaccg
     1561 tgctggcgga ccttatgccc cttgcggact cgggcttcgg tgtcgtcatg tgcaacggaa
     1621 tgaaggtcgg tggcacatac aagtactacg gcttcctctc ggtaccggca ggcagctcaa
     1681 caccggtcga tatccggtcg ctgacctacg acggcggcag gtggcgcgtt gacgacggtg
     1741 cacgcctcag cctggactca tccaccggcc aggagtcggc gtatgtgaaa gaccccggtg
     1801 tcagcgttca ccctgccggc gaagccgccg ggtatgtgat gatttatgtg accgccattc
     1861 cgtaatggac gccccggcaa ggtcaagagc ccaaccgatt tcggcggaag tcgaaacagc
     1921 caacggttta ccaccggcgg gaagctcctt gatcgccggt agtcgatatc gttgatgtcc
     1981 cgcaaaactt gttgagcttc acaagatccg cgtgattcga tccgccagca tatcgagtcg
     2041 ctcgcgggtc tggagggcgt agaaatgctc gcgcccggcc gcgatccggt tgctttcgac
     2101 ctgacgggcc aggaagtcgc ggcggtcggc caacgcgcgc gcgatttcgc catgcgactc
     2161 cgccatccga cgattgaccg cgcggatggc gttgaagacc tcgcgcctct ctaatcgggg
     2221 tccccgtgct tcccgcagtt ggtcggatct gcgaatgagc cgctcacgtt cccgcaagag
     2281 tgacgccggc aagtccggac agtatcgttg cggattgaac caccaatcgc gcacccggcg
     2341 tccggcggcg cgcagatcat cgacggtcgc gggcaggttg ggccatggcg atctcagcgt
     2401 cgcgctcaca cagacatatg ccggcggctc gcaggcgtag taacggcgaa agatgccgtc
     2461 tgtgatgcgg tcgtacttgg ctccgccgat gccgtgaacg aagagatcgc aagccagcag
     2521 ccgcgcccag agcgttagcg tcagcgctcg cggccggatg acccacggcc gcagactctc
     2581 ggccgccgcg gcgaaatcgc gggtcagatc cgccctgctc aattctccga tcgcgttttc
     2641 ctcggcatag cagcggagcg catcaccctt gggctcgatc cacaatcgtc ggcgtcgctg
     2701 tagcggctgg taggcccaca acgcggtttc gattcgaccg ctcacccggg ccaggtccgg
     2761 cagcggtcga tccgccgagc gaattgcgag gtccttgcga tactcggcca gtgacttgtt
     2821 gtacgcatcc gcgaatcgat cggcgttaag caacaggtcg gcgacaaacg gcccgccgac
     2881 aaccttgctg acccgatgtt ccggcaggtc ggcgcccagc tcgcgatcga ttcgcaagcg
     2941 accggcgaga tgctgatcga cagcgtcacc gcgacccgcc cactcggcca ggccgtccag
     3001 gtaagccggc atcagcgtgt catccaggct ttcccccaga acgacggcca gttcctgtct
     3061 gatctccgcg atacgcatgg cggtgagcgg tgttcgaccc tcgtaggctg agccgggcgg
     3121 cgccgtcgtg aagggcacct cccgcaccgc cagcaggccg tcacccccgc gaaccggcac
     3181 tcgcaagccg gcggatcgag gggcgtcatt gtccacgacg agatcaatgc cggacatgcc
     3241 tgccgtgtcg gcaaacgccc ggaccgcaac atgcttggcc cagacgcccg gatgaacgaa
     3301 ctccggctgg tgaccggctg cgacgatcct cgcgttgccg gcgaccccga gcagttgcga
     3361 gcgcacctgt tgccgcgcct cggttattgc tgtgcccgcc agcgccccgc cgattttcga
     3421 cagcagccgg acgttctgct cgaggagggc gcgccaatgc tcaggcggcg gttcaagcag
     3481 aatgccgccg tcgtcgggcg gtgtctggag gctgtgaagc cgaagcattg ctactcaacg
     3541 ggttgcgcca ggccgctcgc cagtccacaa gcctgtcggg gggcggcgct ctcaggatcg
     3601 atctcggccc gcaccggccg atcgatctgc tcggacaatg cgtgcacgag cacatcgtaa
     3661 tagtacagca gccgggcgtc cggatcatcc agcgagccgc cgaagtagcg gttggggtcg
     3721 ttgtagatca gccggacggg gatctccctg attcgcagcc cgcgcctggc cgcctggacc
     3781 cagaactgaa tcggcatggc gtagcccggg acggtgatgt tgagtctcgc aagggccgag
     3841 acccgatagg ccttgaaacc gcagaacgcg tcggtgatcc ccagcctcag aatctcatta
     3901 agcatgtcgg tcatcttctg gttgatggct cgccggtcag cgggcggtcg cgagttgccc
     3961 ggcagcggct gcaggtagcg cgagccgctg ataatgtcgg cgtcgtcctg ccgggccatc
     4021 tggagaaaca ggggtatcca agccggttcg tgctgctcgt cgcagtccat tgtgatgagc
     4081 cagtcgtagt cgcgggaaac ggcaaaccgg aaggcgtcga tcaggctctg accgtagccc
     4141 cgattgtcga tgtgccggat caccgaaatc ccgggttcgg cctgcagaag cctcggggtc
     4201 tggtcggtgg aaccgtcgtc gaccacaaga atgtcgccgt ccggagcgta ttgcctcact
     4261 tggcgcaaaa cgccgatgac ggtctttgcc tcgttgtaaa ccggtatggc taccaggtat
     4321 cgcatgacgc acctcgggga cactcactaa caggaatctt aggccgcccg actccgaagg
     4381 caaacaccgg ttctgaagca aggcaggctc gcggctggag ccctgcgaag tcctacggac
     4441 caagcagggc gggaccctgc tgcaggtgct tcgcgcatac cgcttgccgc cagcccattc
     4501 aaggctgcgt tgacgtcgcg aggtgtgaca ccgccgatgg cctcgatgta gcccccgggc
     4561 gaagcacgcc ggcgacggcg tcaagccggg cggtgccttc cgagagcgtg ggggcgagcc
     4621 agccgccctc gtcgaactcg ttccacgcgt agatgatcgc cgcctgggcc ggggcggcat
     4681 ggcgatgcgt ttccagccaa cgcagcccgc ggcggagatg atccgcaatc gcagcgggcg
     4741 tggccggctc gctgcggtcc acctcgccgt cgtgacttcc gtagggactg ccccacggca
     4801 tgggtttctc gacgcgaggc cgcgggtccc accccgtcac cacaatcggc acaacctgtg
     4861 ccccggtcct gcggcattcc tcccagaagt gctcgaggtt ctcgaccatg cgggcgtagg
     4921 ggatcacgcc tttgcccaga cccgccacgt agctgctgat cgcatcgcaa cccagtgcct
     4981 ctgcccactt cttgccttgt tgagggctga aatccatgat gacgatgtag ggcctgggaa
     5041 gccctctggc cgtcacggtc gctcgaaagg tatcgaggac tctccgaaat ccctcgacgc
     5101 cgccaagctt ctccagccac tgctcattga tgaagcccag atacagcaac ggccgcccgt
     5161 cgagcacccg ctggtatccc ggctcctgca tcaggctgac cacgcggtcc acgaatgcct
     5221 ggtgtggcca tcgtccgcat tcggtgatga gacagaagcc gatcttcgag cggacagaac
     5281 tgctcagata tcgccgcagg gccaggctca atgggtcgtc cgcggggtag gtcacgaacg
     5341 cccagtagtc cagtcccgcg gccgcggcgt actcgatttc gcgatccatc accgcctgcg
     5401 gcgtgccgtc gatctcgatg gcttcatcac cgttcacctt ggcgtagaac ggcagccggt
     5461 tgtgccattt cgacggcccg aggctccggt gaaccgcctc gccgacaacg ccgtggttgc
     5521 cgaaccatgc atcccagcgg atggccccga cgataggacg aggggcttca tgggcgggcc
     5581 tgctctgttg cgcggacagg ccctgcgcga aggcgccatg caccaacaac gcgaagaaga
     5641 gagcgattgt gccaccaggc ggcacccgaa cctcgccgtg ctcatcattg acggatgggg
     5701 ccaggatgta cgagactccg tcctttggcg tctggttctg ggccaaccgt gcttctgaaa
     5761 gcatttgatc gctcctgctg gtcggggctc gtcgacactg cgggtgttat tcgtcccgcc
     5821 tgacagcttg ttcgagggta gccttttcgg ttggcggctc tcgccaaccg ggcgggtgcc
     5881 cccgccgacc tgcccaaagc aacgttgcgt ggcctgcccg ccggtgcctg gccgaccgaa
     5941 aacggccaga gcaaggtgcc atggaacacg gcctgtggta tgataccact tggagtggag
     6001 accggcattc gccgggcagg caaaccggac aagcggacgg cacttcgctc gtcgctcgcc
     6061 ggccgcggtt gcgtgcggtc agacccggcg atgaaggaca aggaactcat acctctcttc
     6121 atggaggtgt cgacatggca ggcaagacca gcccaccgcg caaatcgtcg tccgtgaccc
     6181 cgcgagccgc ccgcaagaag aaggggctga cgaagccgag caggagcacc gccaccaagc
     6241 ccgccaccca ggccaaaggg cctgccgcgg gcggccgccg cccccaagcg gccgacgacc
     6301 gttcgccggc cggtctcctg gcccaagcag aggcggacct gaccgctctc ctggattcgc
     6361 tgaacagcca catggccact gcggtaaatg cgatcactga actggccgtc gcccagcagg
     6421 gccagcatca gcccatcatc cgcagcaggc cgatcgaccg cgccaccgcc atgtttcagc
     6481 gcctggtcgc cgaggttctt gacgacaagc tcgccgaggt tcttcctact ctcgtggctc
     6541 tgcgcagcga gatgaaccag cgtgcccggc tcgtcggctc gtcggcttcg acggaatcga
     6601 ccccgccgac ggacggcgac ttcttcgaac gcggaaccgc catgctcgac caagtcttga
     6661 acaccctcga agtgcgcagc ttcgagccgc gcatcggcga tccgttcgat ccgctgattc
     6721 atctggccgt gggacagact agccgaaacg acctcgccga gggcgtgctg gccgagatcc
     6781 tgcaacccgg tttccgcacc gcgcgcggca aggtgattgt gcccgcccga gtcaaagtca
     6841 acaggaggtg aacggatggc ccggcttggc atcgacttcg gcaccacgaa caccgttgcg
     6901 gtgatccacg atcgcggtgt gttctccgtt gttctccatc gtgcggacac gggagcgggc
     6961 accattgtgc aggatgtctt tccgtccgcg atcttgatcg acaagcgctc aggcaagcat
     7021 tggttcggtt tggaggccga tcgtcttttc ggccaacgcg ggccgggccc cgatcacgtg
     7081 ttcctgccgt cgctgaagcg gcacctgtgg agctacgcgg agggtcattc agtcagctcg
     7141 ggtcacgagc cccgcccccg tgctcgagat gttgccgccg gattggagca gtttgacatc
     7201 ggcaagctgc tgacgggctt cctgcaatct ctggccgaat cgatccgggc atccggcatg
     7261 atcgacccgg gcgagccgct cgagacggtg atcacctggc cggccaacgc caacggcgcc
     7321 cagcgctaca tcacccggaa gtgttttcgc gaggccggct tcgacgtgct ctcgacattg
     7381 aatgagccga cagcttccgc gatcgagctg gctgactgca tcaccgccgg ccgcggcgcc
     7441 gggaaggatg atcgcgaacc ctcggccgtg gccgtctttg acctcggcgg cggcactttc
     7501 gacgcctccg tggtctggat cgaaggggac gagttcgagg tgctggcctc cggcggtatt
     7561 gaagacctcg gcggtgacga ctttgatcgc gccctgttgg acatgttcct ggaacgattc
     7621 aagctccgtg acgaggccct gagtccgctg acccggcatg ccctgttgcg gcaggcccgc
     7681 tcgcagaagg agaccatcgc cggcggtgtg gttcgcagcc tgttcctcaa tcccctcgat
     7741 ttcgggctgg attgccctcc ggtctcgatt gcggtggagg cctatttcga gcgcatcaga
     7801 ccgatgctca agccggccat cgccatgctc aagaaggtga tcgtctcggc tgccgccaag
     7861 gagcgtcggg ttcgcaccgg ttcaagtctg accgtgtacc tggtcggcgg ctcatccaaa
     7921 ttgcccctag tggcccatat ggtctccgac gccttccccg aatgtcgggt gattctgacc
     7981 gacaagccgt tcacctcggt ggccatgggg gcggcaatct gtgccaccga ccgggtgacg
     8041 taccgtgatg tctttgccag gcacttcggc ctgttgcgac tcaaggacaa cggccagtcc
     8101 gaagtcttcg acatcatctt tccggccggc acgcctatcc cccgcaaggg cgaaaaaccg
     8161 ctcgaacgtt gcgcctggta tcatccccgg cacaacatcg ggcacttgcg atacctggaa
     8221 tgcacctcgg tcggagccaa cggcctgccc gatggcaacg tccgccactg gtcggacgta
     8281 ttcttcccct atgaccctct gctgccgatt gatcaccggg cgtcagtcgc cgatgtggtc
     8341 gccaccaatc aattcgctga catgcctgcg tgtgaggtct atcgctgcga tagtgacggg
     8401 gtcatcacgg tagagctgcg ccgcccgaca aagaacgact cccgcatcca cgagatttat
     8461 cgggattgat ctcagcatcg caacggcggt ggtgaagatg ggatcgctcc cttcgttttc
     8521 tgacggactt ccgtccgcaa aaggctcgtg ggttccgggc gtcaccggtc ggttgtctgt
     8581 gccgttggag gggtcaagct tcctcccaac acctgccgat accgataaca atgcattctt
     8641 actatttgtc atggcgttgg ttgagcgcat gggggctggc cttggcatcg gttgtcggct
     8701 gcgcccccaa ggacaccccg tttgcggcca tccgcgccgg catccgctgc gagcgaggcc
     8761 agaaggccct ggagcaggag gatcttgacg ccgccctgaa ggagttccag cgagcggtcg
     8821 agctgaatcc gaagctggct atggcgcatt ccaacctcgg caaggtccag aaggccaagg
     8881 gcaacttagc agcggctgcc gcagcctttg ccaatgcggt gaagctgaac cccaagagtt
     8941 tcgacgacgc gttttcgctc gccgacgtct accacaaaat gtcgaagctc gccgcggcga
     9001 tccaggccta cctgcacgcc tgcgagctcc agccgaataa ctttcaggcg cgcctgaatc
     9061 tcggcgtctg ctatcaccag gccgccgaac tcgacaatgc cattgactgc tacacgaaag
     9121 cgatcgaaat cgaccctgac cacgctgccg catacaccaa cctcggtgcc gcgtacgacg
     9181 cccaaggaaa atactacgag gccatccacg cctacaacaa gtcgctcgag cgcgatggcg
     9241 aacagcctat ggtgctggtc aaccttgccg gcacgatgat gaagcaggaa cgatttgccg
     9301 ccgcccagcg ggccctcgaa cgggcaatcg agctcgatcc cgaactggcc atggcctatg
     9361 aacggctggg ctattgcctg ttccggatga agcgatacga cgaggccctt acccgctacg
     9421 agtgggccga gaccctcgac cccaaccgcg ccgagacgca cgccggcctg ggcgtggtgc
     9481 gaatggcgat gttccttcag gatcgctccc aaactgagtt ccgccaggcg gccatcgagc
     9541 attggcaccg gtcgctggaa atcaaccccg atcagcctaa aatacgcaac ctgatctcca
     9601 agtaccgggt gcccgccgac caggtgtcgg ctgttttgat ggaatcgccg ccaaaatgaa
     9661 aaacaggcaa aatcgagcaa tcgttcagtg cgtactgtca tgcgcgctga tggtctttgc
     9721 tgccggctgt ggtccccaca aggccgctgt tcatcgttcg tgggcggaac gttctcagac
     9781 ggtcatccgg cagaggctgg caccaactcc cgtcgtcccg accatggccg cctcgtcggc
     9841 tcatccggtg aaagcggaga ggacggtcgg cggcaaatcg acgatctggc ccgaggagtt
     9901 ggccgctttc catttgtcag ccgaaacgcg ggaacaggcc ggagacgcgg aaacccgaca
     9961 ggcagaggag agttcctcga agcaggacgt cgaatacgaa ggcaaatggc gaaagccgct
    10021 gccgggcttc ggcgagacac tcaagcgcga cctgctggag ctgccgagcg cgttgtggga
    10081 tgacaccaag cgtgtctaca ccaatccatg gaacctggtg ttcctgctgg gcgcaggcgg
    10141 cgcgtcgctc gctcttcgtc cggaagtcga tgatgacatc gaggattact acgacaggca
    10201 ccataccttc aaagaagact ggcgtgacgc gttcggggcg gccggcaacc cgatcgttca
    10261 tttcggaata gctggcgctt ggtatttgac cggccagctt gcgcaggatg ccaagactta
    10321 tgatgtcggc gcgcggctgt tccgggcatt gaccatcaac ggcgtcagca cgctgctgct
    10381 caagactgcc gcatgcaccg aatcgcctaa cggcgaagac tgggcctggc cgtccggcca
    10441 cgtatcaagt gcgatggtcg tcgcgacggt gatgaacgac gcctacggac cgttggcggg
    10501 ggtgcccttg ttcggactga ccggtctggt ggccgtcgag cggttggatt ccggagaaca
    10561 tcacttctca gatgtggttt tcggtgctgc ccttggctgg gtcgtggctg agacagtcat
    10621 gaaggatgag ccgcccaaga tcgccggcgg ccacatcgtc ccctatgctg acccggtcgg
    10681 ccgcaacgcg ggcgtcgcgt gggtcaaaac gctcggcgaa tgacttcggg gtggtaatga
    10741 cttggccagg gtaggtcgtc cctccacggc ggcggcgttg gccggttacc gcgatcgcaa
    10801 aatccggacg aacgacggcg aaacgcgctc aaacagcgac ggcgcaacgc cgccgatgat
    10861 caacccgagc cgtgccggct cggccgtcga tcactcagga tgggctgtct gctccgcgcc
    10921 ggaaggcgct ccggtctcca gcagatcgct gcaggccgcc gaactccgct cagcctcgtc
    10981 ctggtaactc gccgctatgt ggcgggtgat cacctctgga ggcttataga ggtattgggc
    11041 caccggccgg cggttttcat gcgtgatgct gggggccccg gacttgacga gatcgatcct
    11101 ctcgcccgag ggatcctcga cttgtgcccc tgccttgaga acctggacga ccggctggca
    11161 cggccgtttg gcgttcagat cgacgaccac ggcctctcga ccgtccgaga gcgtcacaca
    11221 ggtaccgacc ggaaaaggcg ggatggttcg cacggccgcc gcgaaaatca ccggatcaaa
    11281 catcttgcgg atctcggccg cttgaagggc tgccagggcg gcgaccttgg gttgcccctt
    11341 ccggtcacaa atggccatca tcccgtccag aacgttggcc accgccacga tccgggcgaa
    11401 gacgtgaatg cggcgaccgc gcagcggctc cgggtgtcgc tccttgtggg tggaaatagg
    11461 ctccggaaac ccgtttccat cgtatcgctg gtggtggtga agaataacac ccgacgctgt
    11521 cgaatcaagc cggccgcgaa gcagttgata gccctgctcg gcgtgagacc ggtaagccgc
    11581 ttcgtcctcg tggtggtcca gaacgtgaga tcgtcgcagt tccggcggca gtttgatctt
    11641 gccgacgtcg tgcagcatcg ccccgacgcc caggttcgtc agatcctggg cgagccgcct
    11701 gtcgatgtac ttacgctcgt gactgatgta gttccgcaga ttcataccca ggatcatcac
    11761 cagataggca acgtttgcgc tgtggctgaa caactcctcg ggttcttcga ggatgcgctc
    11821 cgcccagacc gcgtggtcct ggttcttgac cagttcctct atgagtctcg atatgagctc
    11881 tctgtagaac tggaggtcga aagccccggc ggtctgtttg gccaaggccg tgaaattccc
    11941 tttgatgtcg tggtagagcc tggcccgcga ttcgggaacg ccggaagcga cttgttcgtc
    12001 gagaaagtcg aaacccgggt ggttgatcca tacccccttg atgccgtatt cccgcagctt
    12061 cgcgatggtc ttgggttcca ggacgtatcc cgcggccagc agggcgcgtc tcgggtgcct
    12121 cgcgtgtgga atcgatgctg ccagcaccat cccgggctcc aggttctcga cggagactcg
    12181 gatcatttat cggacgaccc ctaccggcgt gaaagcacta cctccagccg cgacgcacat
    12241 actgctctac ttcatcggct caaatgaggg gcttcgccag cgagttgccg gtcaagtcgg
    12301 cagggcatcg cggtgccaac gttttcggca gttgcggacg ggaagccgcg cattgtggga
    12361 gcatcttttt acgtgtgagt gtattcttgc tcgcggcttg cctcacggct tcgtgatatg
    12421 gcttggctgg gagctgggca cgaagccgcg caccgctttc gtcgaaggcc ggcagcgggc
    12481 cagttcacga tggaccgcct cggcaatgcc ggcggcatcg atgccgcact cggccagttg
    12541 gctcttgcgc gagccgtgac tgatgaagcg gtcgctcggg atgcccaggc gaaccaggtg
    12601 atccgtcgga agccgaagat cctgtgccgt ctcgagcacg gccgctccga aaccgccgac
    12661 caccgagtgg tcctcgacag tgatgacggg
//
LOCUS       DAOTPW010000165        12568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4804_length_12568_cov_5.325901, whole genome shotgun sequence.
ACCESSION   DAOTPW010000165 DAOTPW010000000
VERSION     DAOTPW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4804_length_12568_cov_5.325901"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1053)
                     /locus_tag="P5316_14605"
     CDS             complement(109..1053)
                     /locus_tag="P5316_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86235.1"
                     /translation="MMSGTVRQVHSIRLDADGRIDQDISCLECDYDLRTLRYDGTCPE
                     CGTKVRDAILGDLLHYADAGWMRRLSLGSKIVWWWLSGTVLVFLILLCAIVITSLLGF
                     DQEQQETADPNSSMASLPVSIGVALLLFGWLLAPSVGVWLLTTPEPRSPFSSGDPWLR
                     RALRYGFGSICVLAVLADFLLRRAGHIGGHDPEWSFTPLYCVNGLLLMSYLAHLGKRL
                     PDRSLRILAALTVVGFVAYALLQLVSLLLSIGGVAAGARLNLSAAIPIVGGIVAFVSI
                     GLLHRFAKGLSGAAECAARFTWPPREPKKTEERSAVEQ"
     gene            1180..1944
                     /locus_tag="P5316_14610"
     CDS             1180..1944
                     /locus_tag="P5316_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR86236.1"
                     /translation="MPNIIACRLASYGNYQDRAWTHLPEIGIRNVEIPAPSPEELPAV
                     KRRLADHDLTATSLQGKADIKEPNAAEVLKPQFEACAALGAKVLFLSLKAGETDRSVV
                     WQRMRAIGDLARSFGVTVGVETHPDLVTNGDVGLETMKAVNHPNVRINFDTANIYFYN
                     RNRTAVGELIKVIDYVASVHLKDTPGGFEEWTFPVLGTGVVDFPVIFKMLGDRGFKGP
                     YTMELEGTQGVDRNEAEQLEYIADSAAYLRRIGVLT"
     gene            complement(2418..3899)
                     /locus_tag="P5316_14615"
     CDS             complement(2418..3899)
                     /locus_tag="P5316_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR86237.1"
                     /translation="MKYLKLALVLLLSAPLVGTSCGTDNPLLLPSQDSPDGDQTAGLV
                     LVARLVHVTDTHVVDPLSPARFPGAHEFTLSAWRPWESYSTQILDGIVRTANRIHASG
                     TTVDFLIHTGDACDNAQSNELRWFLDVMDGREVNPLSGPDDRPVESRPPTVLDPYATF
                     RPEGLYRSGVHGNLPSIPWYCLIGNHDRFATGLLPIVSLPGGRRVAPLPFFDRPGLLL
                     PTVFDPTGRLAHGNVTPAEPGPPRLFEPPVFVAPNPDRAYFNRNEFIGAMFGSDSEPA
                     GHGFETADGRGYYSISPAPGLRLIGLNTSDQPAPLPTGIYEAGCISDEQSEFLRRELA
                     SAAERGERVIVASHHPSHTLEVLHGSALGPERFRALLNDHPNVILHLAGHLHRNRVAD
                     RGGYIEIETCSTLDPPQEARVVELWRDPVDDSLLIRYYMFSHLNDELPPLGEDPLRGL
                     REQAWQLAEGYRRGVLRQAATNDLVSDPLGTMEDRQGWIRLDH"
     gene            4128..4862
                     /locus_tag="P5316_14620"
     CDS             4128..4862
                     /locus_tag="P5316_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HRR86238.1"
                     /translation="MEPPEPAGLPIPSEVRDPQYRIIGWSVEDRPIECRILGDGPDVT
                     LFIGTIHGNEPSGTPLIRRLADYLAERLDLLDGRQVILIPALNPDGMVRHSRFNARNV
                     DLNRNFPVGNWKPDRHSGQHPLSEPESQILHDLLHIHQPCRVITIHETLACIDYDGPG
                     EALARAMAACSDLPVRRLGGLPGSLGSYVGMILHRPIITIELRRFAHRLPEAELWNRY
                     RDMLLAAITFEDNRTATAGDVFWMRW"
     gene            complement(5088..5936)
                     /locus_tag="P5316_14625"
     CDS             complement(5088..5936)
                     /locus_tag="P5316_14625"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR86239.1"
                     /translation="MIELGMHVDNWRHQDAPYTVPCEFAKKHGMKYIEFGSIWGDYFV
                     QALGYNPHIPLHTDPLRLKKYVDSMGLKVSQIDAAYPVSLPEGLYRGIPYTIEAIKFA
                     RSLGAQFVDTTDGATKPQGLSDDEVFDLMKHYYRIVLQWAESHEVIVNVEPHGPYTNN
                     ADFMERLFTEFDTPLLRMNFDTGNNFIAGNDPVAFLKRFRHKVSHCHIKDVSESLAKA
                     VRGEETGISTSVVAIGEGVNADNIAGCIELLKSTKWSGVLSIECEAAPGKVEQSLDWL
                     RKQIAK"
     gene            complement(6352..7560)
                     /locus_tag="P5316_14630"
     CDS             complement(6352..7560)
                     /locus_tag="P5316_14630"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86240.1"
                     /translation="MSEHIEMTRMGRRGFLAGTAATAFTVVSPSLVRGAETNSKIELG
                     LIGCGGRGGWIADLFAKHGGYKLVACADYYQDRVDALGEKFGIDPGRRHAGLSGYKKL
                     LEGKLDAVVIETPPYFHPEQAADAVDAGKHVFIAKPIAVDVPGCLSIGESGKKATAKK
                     LVFLVDFQTRANEFYREAAQQVHDGVIGKLVCGEARYPWAGGVMPAPKDKQDRLRLWY
                     CEKALSGDYIVEQNIHTLDVATWFINADPVKAVGAAASKGLRTYGNIKDCYSVTFWFP
                     DDVLLNFMSVQCVPGVPDSIHCRIYGKDGVVDTDYYSHVWIRGKTPYKGGEFKGLYQE
                     GAVVNIKEFHQFVTEGNYANPTVAPSVRSNLTCVLGRMAGEKAGQVVTWDEMIKANEK
                     LEFDLSGFKS"
     gene            7816..9516
                     /locus_tag="P5316_14635"
     CDS             7816..9516
                     /locus_tag="P5316_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86241.1"
                     /translation="MNRSSSAFRWSLTLSAVLFSSTTALSAETSTAQCGRYRVLTANA
                     HKAPPFEVVDICVAEKPQSGPCLWQIDIHAKDGADTPPLMQLRAVTSENPLSEPSKPP
                     VFDKYILRVPAIDETIEYRNVHTGKALLPSWGDFARHFIPHAARGTGRQRGLPNTCEY
                     LGHVLSLRSVSKKDLPAWEKVTVLNLDPELLIGTGRTFKDKEGHRLPQHPERRDYTYI
                     PWTQEDYVAMIEAGHNYFALVPGILEWLRAQPVFYRAEASIQYPTDLYRSNMLGPAMF
                     IDEPTCIMTGDKEVINLLHFATDATALITKRVRAEAEQVMYHYESTLRKGVNFGDMRL
                     SQPDFASWETRYETAFYQLAGGTAGIVHEGRYQLGEFNDNLKASTGIDRPMTAEQMYR
                     YYYAFLRGAARHFNKDWGTSIYGQADPKISPLAIEMAYDMGARYIWYWTSDHDHHMPF
                     PEQLDLTRSLKKHMAAKPRPSIRTARPTLDKAIVVPYGYVIALESPTNRKNAWDLWWV
                     REMDADGKNEASQRYYRLRRAVLEEVFKSLDANEEFDIIHDDGGEITGYRQLVRLKAE
                     "
     gene            complement(9550..10905)
                     /locus_tag="P5316_14640"
     CDS             complement(9550..10905)
                     /locus_tag="P5316_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002732649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR86242.1"
                     /translation="MIISIWRRFFAPAPHIPRLPDDQVRRVYPYYRWRVMEATYIGYA
                     TYYLVRNNINVVTKDMETALGYDDSMLGSLLAINAIAYGLGKFVMGALSDRSNPRVFM
                     GVGLLLTAFCNFAFGSVASYPVHLFLWATNGLFQGMGWPPCGRSMGHWFSEKERGLTF
                     SIWNTAHNVGGGVAGVIAGYALHYCGGWQFAFFIPGVLATIGGLYVLFRLCDTPQSVG
                     LPPIEEYRQDYPNGARPGLDAERELTTRELLVDMVFTNKYIWILAAANFFAYVTRYSM
                     LDWGPKYLREIKGATEIGGGVGTMFLEFGGIPSTIILGWVSDLLKGRRGMVAVLCLAP
                     IIVAFTVIMFTPAGYLWLDMAMLTLIGFLIYPVINLIVIQALDLTSKKAIGTAAGFIG
                     LMGYFGKATQAKGFGWILYHLKPVYGQETAWMAVMAAILLCTAIGVVLLSFTWRLKPR
                     A"
     gene            complement(10902..12425)
                     /locus_tag="P5316_14645"
     CDS             complement(10902..12425)
                     /locus_tag="P5316_14645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRR86243.1"
                     /translation="MSEMMSEERVKTVSELVSEPLDVLVVGGGIVGTGIARDAAMRGL
                     RVGLVEQHDFAFGTSSRSSRLLHGGLRYLAQGRIGLVREASIEKRIVHALAPHICQPL
                     AFVLPTYRGMPWAPWALWKLRIGVRIYDWLCGGRNFGNSSSMKPGQVVDYLPGVNDRN
                     LTGAVRYFDGLTNDARLTIDTLRSAAKAGAKVLNYCKLETARHESGKWHCRLEDRVSG
                     DEVSIISRCVVNATGPWGGQFKQSTIPLRLTKGIHLVVERFRLPIPDAVVMTEGKRIL
                     FAIPWGERVILGTTDTDYDGSLDEVFADPDDVSYVLGVVNEAFPRASLTTEDIVSTWA
                     GLRPLIADPNGRPSDISRSHEIRMSEPGWYNVAGGKLTTYRLMAEQTVDQIVRYLGRG
                     RVPCNTAATPLLEPLEVENLSGIVPPPVSARAVEHYCRREWAIHLDDVMIRRAGWHYY
                     RRQAEGIADQVVRWMAETLGWDAARQAAEIQQYRRQVGRSSPAGADRRLPVETGVRA"
BASE COUNT         2559 a         3945 c         3761 g         2303 t
ORIGIN      
        1 cctgaacacg aaccaacagc atacctctcg gaggcttgct agatgattat cagctatcag
       61 ccaccggtga cagaagtggc aaccatggtg acatgccagg aacccccgct actgctccac
      121 ggctgatcgc tcttcggttt tcttcggttc ccgtggcggc caggtgaatc gggcggcgca
      181 ctcagcagcc ccggacaacc ccttcgcgaa acgatgcaga agccctatgg acacgaacgc
      241 gacaattcct ccgacaatcg gaattgcagc ggagaggttc agtcgagcgc cggccgccac
      301 tccgccaatc gacagaagaa gactgacgag ctggagtagt gcatatgcca cgaagcccac
      361 gactgtcagg gcagcgagaa ttctaagcga ccggtcgggc agccgcttgc ccaggtgagc
      421 aaggtaagac atcagaagaa ggccattcac gcagtaaagt ggcgtgaaag accactcggg
      481 gtcatgtcca cctatgtggc ctgcacgacg cagcaggaaa tcagccaaga cggccaggac
      541 gcagatactg ccgaagccgt aacgcaaggc acgcctcaac catgggtccc ccgaagaaaa
      601 gggtgatcgc ggttccggcg ttgtcaggag ccaaacaccc acggatggcg ccagcagcca
      661 tccgaaaagg aggagcgcaa cgccgatcga gaccggcaga cttgccatgc tggagttcgg
      721 gtctgctgtc tcctgctgct cttggtcaaa accgagcaga gatgtaatca cgatggcaca
      781 cagcaaaatg agaaagacaa gcacggtgcc ggacagccac caccatacga tcttcgaccc
      841 caaggataac cggcgcatcc agcccgcatc tgcataatgc aggagatccc caaggatggc
      901 gtcacggacc ttcgttccgc actccgggca agtaccatcg tacctgagcg tgcgcaagtc
      961 gtagtcgcac tccaggcatg agatatcctg atcgatgcgt ccatcggcat caagcctgat
     1021 cgaatgaact tgccggacgg tcccggacat catgaagccc ctgtcttgta ctgagtcccc
     1081 gggcagggtc cgcctgaacc gccagactct ggacgtttac gggggtgatg gtacactccc
     1141 gatcagagat tagcaacagc cataaaacgg agttgcctca tgccgaacat catcgcctgc
     1201 cggctggcca gttacgggaa ctatcaggat cgcgcgtgga cgcacctgcc ggagatcggc
     1261 attcggaacg tcgagatacc cgccccgtcg ccggaagagc tgccggccgt gaagaggcgg
     1321 ctggctgacc acgacctgac ggccacctcg ctgcagggca aggcggatat caaggagccg
     1381 aacgcggccg aggtgctcaa gcctcagttt gaggcctgcg ccgccctggg agcgaaagtc
     1441 ttgtttctga gcctcaaagc gggcgagacg gacagatccg tggtgtggca gcggatgcgc
     1501 gcgatcggcg atctggcccg gtctttcggc gtgaccgtgg gcgtcgagac gcaccctgac
     1561 ctggtgacca acggcgatgt gggtctggag acgatgaagg ccgtcaacca tccgaacgtg
     1621 cggatcaact tcgatacggc taacatctat ttctacaacc ggaatcggac ggccgtaggc
     1681 gaactgatca aagtcatcga ctacgtggcc tcggttcacc tcaaggacac gccgggcggg
     1741 ttcgaagagt ggaccttccc ggttctcggg acgggcgtgg tcgatttccc ggtcatcttc
     1801 aagatgctcg gcgatcgggg cttcaagggt ccctacacca tggagcttga gggcacacag
     1861 ggcgtggacc ggaacgaggc cgagcaactc gaatacattg ccgactcggc cgcctacctg
     1921 cggagaatcg gggtgctcac ctgagtctct aagtggtgcc gacgcccccg tcggcacgtg
     1981 tttagcgcgc aagacagcgg gtcaggcact atttgccatc ttcttcctgc cgccgagtcc
     2041 ccgcatgact gagccaacca ctccagattg cacggcgaac cgcatttggg caaaaagagc
     2101 cagaccccag cacgctaaac acctaccccc gtcagcacgg attcccgcca aggtcctcat
     2161 ccgccatgcg gcacgttcgg cagcagtcct gtgatcgacc ggccttggcc gcctgggccg
     2221 gtgagggcac cggccccact gtggtgccga cgcccccgtc ggcactctcc gacagaaaga
     2281 gtggacgcaa agggagagaa gagaatagta acacacgaag tctcacagaa tcgccacgat
     2341 gggccgttgt cggtcgggga tttcaaatca caaatctgag atttcagatt cacagaagcg
     2401 ccacaggtga cctgttatca atggtccagg cggatccagc cttgacggtc ttccatcgta
     2461 ccgagcggat cggataccag gtcgttcgtc gccgcctgcc gcagtacacc ccgcctgtag
     2521 ccttcggcga gttgccaggc ctgctcgcgg agtccgcgta gagggtcttc gcccagcggc
     2581 ggcagctcat cgtttagatg tgagaacatg tagtacctga tgagcagcga gtcgtcgacc
     2641 ggatcacgcc agagttcgac gacgcgagcc tcctgcggcg ggtcgagcgt gctgcacgtc
     2701 tctatctcga tgtagccgcc tcggtcggcc acgcggttgc gatgcaagtg cccggccagg
     2761 tgaagaatca cgttgggatg gtcgttcagc aaggcacgga atcgctccgg accgagagcc
     2821 gacccatgca gtacctccag ggtgtgactg ggatgatgcg aggcaacgat gacccgctcg
     2881 ccgcgttccg cggcactcgc cagttcgcga cgaaggaact ctgattgctc gtccgagatg
     2941 cagccggcct cataaatgcc cgtcggcagc ggcgcaggtt gatcactggt gttcaatccg
     3001 atcagcctta gccccggggc cggcgaaatg ctgtagtatc ccctgccgtc cgcagtctcg
     3061 aagccatgcc cggcgggttc ggagtcactg ccgaacattg caccaataaa ctcgttccgg
     3121 ttgaaatagg ctcgatccgg gtttggagcc acgaacacgg gcggctcgaa caatcgcggc
     3181 gggccgggct cggccggcgt gacgttgcca tgagccagcc ggcccgtggg atcaaatacg
     3241 gtcggcaaaa ggagaccggg gcgatcgaag aacggcaatg gtgcgaccct gcgcccgccg
     3301 ggaagagaaa caatcggcaa gagacccgtg gcaaatcggt cgtgattacc gatcaggcaa
     3361 taccagggta tcgagggcag gttcccgtgc acaccggatc ggtagaggcc ctccggtcgg
     3421 aacgtcgcgt acgggtccaa aaccgtcggc gggcgactct cgacggggcg atcatcgggc
     3481 ccggacagag gattcacctc gcggccgtcc atcacatcga ggaaccatct caattcgttc
     3541 gactgggcat tgtcgcaggc gtcgcctgtg tgaatgagga aatccacggt cgtgccggat
     3601 gcgtggatgc ggtttgcggt ccgcacgatg ccatcaagga tttgtgtcga atagctttcc
     3661 caaggtcgcc atgcgctgag cgtaaactcg tgagccccgg gaaagcgagc gggggaaagc
     3721 gggtcgacca catgagtgtc ggtgacgtgc acgagccgag ccaccagcac cagtccggcg
     3781 gtctgatcgc cgtccggact gtcttgggaa ggcaacaaca gcgggttgtc cgttccgcac
     3841 gaagtaccga ccaaaggggc tgagagcaac aagaccagag ccagtttcag gtacttcacg
     3901 gatactatgt taggcgcatg gccgtgcccc taccaccccg tcgccggtcc aaagtagaat
     3961 gcgccaagtc ccgtggggaa agcatgctgg aatggagttc cttgatcgtg ccgacataca
     4021 tagacacagc gaactatcga acggcttggc cgtggtctcg tgttattccg gctttctgcg
     4081 cggcgttgat cttgtcggcg accggatgct gtccgaccgg aaagcagatg gagccgccag
     4141 aacctgctgg tttgccgatt ccgagcgaag tgcgcgatcc gcagtacagg atcatcggct
     4201 ggtcggtcga ggaccggccc attgaatgcc gtattctcgg cgacggaccg gacgtcacgc
     4261 tgttcatcgg cacgatccac ggcaacgagc catcgggcac tccgctgatt cgccgcctgg
     4321 cggattacct cgccgaacgg cttgatcttc tggacgggcg gcaggtcatt ctgatccccg
     4381 cgctcaaccc cgacggcatg gttcggcaca gtcgattcaa cgctcgcaac gtggatctca
     4441 accgcaattt ccccgtcggc aactggaagc ccgaccggca ctcggggcag catccgctgt
     4501 cggagccgga atcccagatt cttcacgacc tgctgcacat acaccaaccc tgccgcgtca
     4561 ttaccatcca tgagaccctg gcgtgcatcg actatgacgg accgggagaa gcgctggcga
     4621 gagcgatggc tgcttgctcg gatttaccgg tccggcgtct cggcgggttg ccgggttcgc
     4681 tggggtctta cgtggggatg atcctgcacc ggcccatcat caccatcgaa ctgcgtcgtt
     4741 ttgcccaccg tctgcccgaa gcggaactct ggaatcgcta ccgcgacatg ctcctggcgg
     4801 cgattacctt cgaagacaac aggacagcga ccgcgggcga cgtgttctgg atgcgctggt
     4861 agcagctcac gcggccggtc ggctttccgg aagctgattg acccccttac cagcgcaaaa
     4921 cgggaacggt attcgggcgt ccgtgttgat caatgccctc atcgctaccc cacctcgaaa
     4981 acgagatagc gcacccggcc ggatcggagt tctcagttgt cagtccggag tagagccgcc
     5041 gatgcgattc ttcgactgat cactgacaac tgaacactga caactactta cttggcaatc
     5101 tgcttcctca gccaatcgag gctctgctcg accttgccgg gggcggcctc gcattcgatg
     5161 ctcagcacgc cgctccactt cgtgctcttg agcaactcga tgcagccggc gatattatcg
     5221 gcgttgacgc cctcgccaat ggcgaccacc gacgtcgaga tgcccgtctc ctcgccgcga
     5281 acagcctttg ccagcgattc gctcacgtcc ttgatgtggc agtgcgagac cttgtggcgg
     5341 aaccgcttga ggaaagccac ggggtcgttg cccgcgataa agttgttgcc ggtgtcgaag
     5401 ttcattcgca aaagcggcgt gtcaaactcg gtgaacagcc gctccatgaa gtcggcgttg
     5461 ttggtatagg ggccgtgcgg ctcgacgttg acgatgacct cgtggctttc ggcccattgg
     5521 agcacgatcc tgtaatagtg cttcatcagg tcaaacactt cgtcgtcgga caagccctgc
     5581 ggcttggtcg cgccgtcggt ggtgtcgacg aattgggccc ctagcgaccg cgcgaacttg
     5641 atcgcttcga tggtgtaagg aatgccgcga tacaacccct cgggcaagga aacggggtag
     5701 gcggcatcga tctgcgaaac cttcaggccc atcgagtcga cgtacttttt gagccgcagc
     5761 ggatccgtgt gcagcggaat gtgcgggttg taacccaggg cctggacgaa atagtcgccc
     5821 caaatgctgc cgaactcgat gtacttcatc ccgtgcttct tggcaaactc gcacggaacc
     5881 gtatacgggg catcctggtg tcgccagtta tcaacgtgca ttcccaattc gatcatgatc
     5941 tattcctccg agcaccgccg tatgctccct cgtgatcccg gcaggcggca ttcctgaaac
     6001 tcgccatccc gctcggcccg gaatcctctc cggacctcct ctctctgtgg aatccgttcc
     6061 gctcagcccg gaatcccttc ccaaccgccc ccctggccgc tcggcccgga atcccttccg
     6121 ggccgcctct ggtggcggaa tcctttccgc attcggtgag aatgaggata ggaatccgcg
     6181 gcccagaagg ccccggaacg gattccgggg ccggcagtct gagatctcag atctgcaatc
     6241 tcaagcctgg aatctcggat ctcaaatctg aaagctcgaa tctgacatcc gcaattctca
     6301 actcgcaatt ctcaattgcc cggtgttgcg gcacgcaacg tcggaggcaa ttcagctctt
     6361 gaagccgctc aggtcgaact ccaacttctc gttggccttg atcatctcgt cccaggttac
     6421 gacctggccg gccttttctc ccgccatgcg gccgagaacg caggtcaggt tgctgcgaac
     6481 cgatggggcc acggtcgggt ttgcgtagtt gccctcggtg acgaattggt ggaattcctt
     6541 gatgttgacc acggcccctt cctggtagag gcccttgaac tccccgccct tataaggggt
     6601 tttgcctctg atccagacat ggctgtagta gtcggtatcc accacgccgt ccttgccgta
     6661 gatacggcag tgaatcgaat cgggtacccc gggaacgcac tgcaccgaca tgaagttgag
     6721 cagaacgtcg tccgggaacc agaaggtcac cgagtagcaa tccttgatgt tgccgtaggt
     6781 ccgcaggcct ttgctggcgg ccgctccgac cgccttgacc gggtcggcgt tgatgaacca
     6841 tgtggccacg tcaagcgtgt ggatgttctg ttcgacgatg taatcgcccg acagggcttt
     6901 ctcgcaatac cagaggcgca gtcggtcttg cttgtctttg ggagccggca tgaccccgcc
     6961 ggcccagggg tagcgggcct cgccgcagac gagctttccg atgacgccgt cgtgtacctg
     7021 ctgagcggcc tcgcggtaaa actcgttggc tcgcgtctgg aagtcgacca ggaagacgag
     7081 cttcttggcc gtggctttct tgccggactc gccgatcgac aggcagcccg gcacgtcgac
     7141 ggcgatcggc ttggcgatga acacgtgctt gccggcgtcg accgcgtcgg ccgcctgctc
     7201 gggatggaaa tacggcggcg tctcgatgac caccgcgtcg agcttgcctt cgagaagttt
     7261 cttgtatccc gacaggcccg cgtgacgcct tcccgggtcg atgccgaact tctcgcccaa
     7321 cgcgtcgacg cgatcctgat agtaatcggc gcaggcgacg agcttgtacc cgccgtgttt
     7381 ggcaaacagg tcggcgatcc atccgccacg gccgccgcaa ccgatcaggc ccagctcgat
     7441 cttcgagttg gtttccgctc ctcgcaccag cgaggggctc acgacggtga acgccgtcgc
     7501 ggccgttcct gccaggaagc cgcgccgtcc cattctggtc atctcgatat gctcggacat
     7561 gattcgcctc caatcgctcg gcaacgtgaa tttcgctcat cacccactag gaccgggcat
     7621 tctaaccggg ggccatggag aggccaaggg ggcaggcgaa tgatgaatga ggaattgcgg
     7681 aatgatgaat ggaaaacgcg gtcgggcttt gcccaacgtt tgcctcaggg cgcgattcgc
     7741 tgagcgcgat ccttgctcaa gttgcctttc atcgatatcc ttccgtcgga accccaggtc
     7801 gcaggagcaa catacatgaa ccgatcttct tccgcattcc ggtggtccct gaccttgtcg
     7861 gccgtgctgt tctcaagcac cactgccttg tccgctgaaa caagcacggc gcaatgcggc
     7921 cggtaccgcg tgctcaccgc caatgcccac aaggccccgc cgtttgaggt cgtcgatatc
     7981 tgtgtggccg agaagccgca gtctgggcca tgtttgtggc agatcgatat ccacgcaaaa
     8041 gacggggcgg acaccccgcc gctgatgcag cttcgagcag tcacctcgga gaacccgctg
     8101 tcggagccga gcaagccgcc ggtgtttgac aagtacatcc tccgcgttcc ggcgatcgac
     8161 gaaacgattg agtaccgcaa cgtccacacc ggcaaggccc tgctgccaag ctggggcgac
     8221 ttcgctcgcc acttcatccc ccacgcggcg cggggaaccg gtcgtcagcg agggctgccc
     8281 aacacgtgcg agtacctcgg ccatgtgcta agccttcgca gcgtctcgaa gaaggatctg
     8341 cccgcgtggg agaaggtgac ggtgctcaat ctcgatcctg agctgctcat cggcacgggc
     8401 cgtaccttca aggacaagga aggccatcgc ctgccgcagc accccgagcg tcgagactac
     8461 acgtacatcc catggaccca ggaagactac gtcgccatga tcgaggccgg ccacaactat
     8521 ttcgcgctcg tgccggggat tttggaatgg ctccgtgcgc agccggtctt ctaccgggcc
     8581 gaagcaagca ttcagtaccc gacggactta taccgcagca acatgctggg cccggccatg
     8641 ttcatcgacg agccgacctg catcatgacc ggggacaagg aggtcatcaa cctgctgcac
     8701 ttcgccaccg acgcgaccgc cctgatcacc aagcgagtgc gggctgaggc ggaacaggtg
     8761 atgtaccact acgaatcgac cctgcgtaag ggggtcaatt tcggggacat gcgacttagc
     8821 cagcctgatt tcgcctcatg ggagacgcgc tacgaaaccg ctttctatca gcttgccggg
     8881 ggtacggccg ggatcgtcca tgagggacgc taccagcttg gagagttcaa tgataacctg
     8941 aaggcctcga cgggcatcga ccgcccgatg accgcggagc agatgtaccg atactactat
     9001 gcgttcctgc gcggagccgc acggcatttc aacaaggact ggggcacgag catctacggc
     9061 caggcggacc cgaagatcag tcccctggcc atcgaaatgg cttacgacat gggggcccgg
     9121 tacatctggt actggaccag cgaccacgac catcacatgc ccttcccgga acagctcgat
     9181 ctgacccgct cgctgaaaaa gcacatggcc gccaaaccgc gcccgtcaat ccggaccgcc
     9241 cggccgacac tcgacaaggc gatcgtcgtc ccgtacggct acgtcattgc cctcgaatcg
     9301 cccaccaacc gcaaaaacgc ctgggatctg tggtgggttc gcgagatgga cgccgacggc
     9361 aagaacgagg cctcgcagcg ctactaccgg ctccgaaggg cggtcctgga agaggttttc
     9421 aagtctctcg atgccaacga ggaattcgat atcatccacg acgacggcgg cgagatcacc
     9481 ggttaccgtc agctcgtccg gctcaaagcg gagtagcagc ggaagaaccg ccgagacaac
     9541 aagcagatcc tatgccctcg gcttgagtcg ccaggtaaag gacagcagga caacgccgat
     9601 ggcggtacag agcagaatag ctgccatgac cgccatccag gcggtttcct ggccgtagac
     9661 cggtttgagg tgatacagga tccatccgaa gcctttggcc tgcgttgcct tgccgaagta
     9721 acccatcaag ccaatgaagc ccgcggccgt gccgatggcc ttttttgagg tcaaatccag
     9781 cgcctggatc acgatcagat tgatcaccgg gtaaatgagg aagccgataa gcgtgagcat
     9841 ggccatgtcc agccagagat agcccgccgg cgtgaacatg atgacggtga aggccacaat
     9901 gatgggggcc agacacagga cggccaccat gccgcgccga cccttgagaa ggtccgaaac
     9961 ccagccgagg ataatcgtcg acggaatgcc cccgaattcg aggaacatgg tgccgacacc
    10021 gcctccgatc tcggtggccc ctttgatctc gcggagatac ttcggccccc aatcaagcat
    10081 gctgtagcgc gtcacgtagg cgaagaagtt ggccgccgcc agaatccaga tgtacttgtt
    10141 cgtgaacacc atgtcgacga gcagctcgcg ggtggtcagt tcccgttcgg catcaagccc
    10201 cgggcgggcc ccgttgggat agtcctgccg gtactcctcg atcggcggca gcccaaccga
    10261 ttgcggcgtg tcacacagtc gaaagaggac atagagacct ccgatcgtgg ccaagacccc
    10321 cggaatgaag aacgcaaact gccagccacc gcagtaatgg agcgcgtagc cggcaatgac
    10381 cccggcgact ccgccaccaa cattgtgagc ggtgttccag atgctgaagg tcaggccgcg
    10441 ttccttctcg ctgaaccagt ggcccatgct tcgaccgcac ggcggccagc ccatgccttg
    10501 aaaaagtccg ttagtcgccc agaggaacaa atggaccggg tagctggcaa cactgccaaa
    10561 ggcgaagttg cagaaagccg tcagaagaag ccccacgccc atgaataccc gcgggttgct
    10621 gcggtcggag agagcaccca tcacgaactt accaagccca taggcgatcg cattgatcgc
    10681 caggaggctg cccaacatcg agtcgtcgta gccaagggcc gtctccatgt ccttggtgac
    10741 cacgttgatg ttgtttcgca ccaggtaata cgtcgcatag ccgatgtacg tggcttccat
    10801 gacccgccag cggtaatagg ggtacactcg gcgcacctgg tcgtccggca gtcgcggaat
    10861 gtgcggggcg ggagcaaaga atcgtcgcca gattgaaatg atcatgcgcg cacacctgtc
    10921 tcaaccggaa ggcgccgatc cgccccggca gggctggacc gaccgacctg ccggcgatac
    10981 tgctggattt cggccgcctg ccgggccgcg tcccagccga gtgtctcggc catccagcga
    11041 accacctggt cggcgatacc ctcggcctga cgccggtagt aatgccagcc ggcccgccgg
    11101 atcatgacgt cgtcaagatg aatcgcccat tcgcggcggc agtaatgctc gaccgcgcgg
    11161 gcggacacgg gtggtggcac gatcccgctg aggttctcga cttccagcgg ctccagaagc
    11221 ggcgtcgcag cggtgttgca gggtacacga ccgcgcccga ggtacctgac gatctgatcc
    11281 accgtctgct cggccatcag ccgataggtt gtcaatttgc caccggcgac attgtaccag
    11341 cccggctcgg acatgcgaat ttcatgcgag cgggagatat ccgagggtcg gccgttcgga
    11401 tcggcgatca gcggcctcag accggcccac gtgctgacaa tatcctcagt ggtcagcgat
    11461 gctcgcggaa acgcctcatt aaccacgcca agcacgtagc tgacatcgtc cggatcagcg
    11521 aaaacctcgt cgagcgagcc atcgtagtct gtgtcggtcg tgcccagaat cacccgctcg
    11581 ccccagggga tggcaaacag gatccgcttg ccctcggtca taacgacggc atcgggaatg
    11641 ggcaggcgga accgctccac caccaggtga atccccttgg tcagccgcaa cgggatggtg
    11701 ctctgcttga actggccccc ccacgggcct gtggcgttga ccacgcagcg gctgatgatg
    11761 ctgacttcgt cgccgctgac gcgatcttcg agccggcaat gccacttgcc ggattcatgc
    11821 ctggccgttt ccagcttgca gtagttgagg accttcgcgc cggccttggc ggccgatcgc
    11881 aatgtgtcga tggtcaaccg agcgtcgttc gtgagcccgt cgaagtagcg gaccgcgccg
    11941 gtcaggttgc gatcattgac gcccgggaga tagtcaacaa cttgcccggg tttcatgctg
    12001 cttgaattac cgaagttacg cccgccgcaa agccagtcgt agatccgcac gccgatccgc
    12061 agcttccaca gggcccacgg tgcccacggc attccccgat acgtcggcag cacgaaggcc
    12121 aacggctggc agatgtgggg ggcaagggca tgcacgatgc gcttttcgat gctcgcttcg
    12181 cgcaccaggc cgatccgccc ctgggccagg tagcgcaacc cgccgtgaag cagtcggctg
    12241 gaccggctgg aggtaccgaa ggcgaaatcg tgttgctcga ccagcccgac gcgcaggccg
    12301 cgcatggccg cgtcgcgggc gatgccggtt cccacgatgc cgccgccgac aaccagtacg
    12361 tccagcggct cgctcaccaa ttcactgaca gtcttcaccc gttcttcgct catcatctcg
    12421 ctcatttatc cttcttgccc ctgtgtacct cgaccggcaa ttctcgcgtg tccgctggcc
    12481 ccagaatcct ttctggggcc ttccgcacgg ggcggcccgg aaaggattcc gggccgagcg
    12541 gattccgggc cgagcggatt ccgggccg
//
LOCUS       DAOTPW010000166        12552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4813_length_12552_cov_5.365208, whole genome shotgun sequence.
ACCESSION   DAOTPW010000166 DAOTPW010000000
VERSION     DAOTPW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12552
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4813_length_12552_cov_5.365208"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..388)
                     /locus_tag="P5316_14650"
     CDS             complement(98..388)
                     /locus_tag="P5316_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86244.1"
                     /translation="MAFFVSRDLGFLVWDGGLGHGLGSPRVFHIQENPLVRTFYAPDK
                     LVFPPHSRASFPGDPLEIRNLPPWIYSLFALVVTHSVLNDAKVEAFRRRQSR"
     gene            complement(486..1427)
                     /locus_tag="P5316_14655"
     CDS             complement(486..1427)
                     /locus_tag="P5316_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRR86245.1"
                     /translation="MPANLTRRELLAGGLATAVGLRAARADAQTPSSKPYGDFIISLN
                     TSTLRGHKLPIARVIDIAAEAGYQGIEPWVDELDRHVADGGTLSDLNKRIKDHGLKVT
                     GAIAFFEWMADDDARRAKGFDEAKRRMEQLAQIGATHIAAPPAGDVKGVDLLKAAERY
                     RALLELAEPFGVIPAVEIWGASPAIHRLGQAVLIAIEAHHPKACILPDVFHLYRGGSD
                     LADVAHLNPRLLAGFHLNDYPANPPQESIADKHRVHPGDGIAPLKQLVRDLRRIGYTG
                     SLSIELFNPDYYRQDPLLVARTSLDKTRAVIRQAAEN"
     gene            complement(1603..3147)
                     /locus_tag="P5316_14660"
     CDS             complement(1603..3147)
                     /locus_tag="P5316_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86246.1"
                     /translation="MGLLRVDLEETMNTNMSRRELIGASAGLVAASAVGRMSWAAGGE
                     AAGPAAGYTKTRVLRVFIGGTHSGWPRPDLDIPAEAKRLQQEIDKVPGMEDVEFLSNA
                     LIQNVESLDKVLEQHKDVDGILGVIVCIGTSHLVNRLADCGRPTVLYATPYSGHEWCI
                     VPDLQRAGKKIDVIPSSNFADAADAIRPFRAIHRLKETRVLFVSGGRPAPADYVKEVK
                     DKFGTEIIDFDHRKLVEVYNAMDDAAVSADADQWIKNAKEIREPSKDEITKSARMNLA
                     LQKVLADSKAQAITINCLGLFGQKGLPAYPCFGFARLNDLGLVGVCEADLPSTMTQII
                     YLHLTGKPGFVTDPVFDTATNTVIHAHCVSATKMDGPQGEAAPYIIRSHTEDHKGAVL
                     QVAMRVGQEITMAKLVKADPYAPVEKPMLAASPMECYGTGAMLVSTGKIVAVPDVDRA
                     CRTKIAVKVADARKMFEQWSNGLHRVIFYGNHMADTRRLARFTGFDVVEEGCNIRHKL
                     LFGGSR"
     gene            3744..5648
                     /locus_tag="P5316_14665"
     CDS             3744..5648
                     /locus_tag="P5316_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86247.1"
                     /translation="MRRYVLFCLFGLMLTGSIHAEDLVFVQDGWKLKVSDLGNIVGLA
                     NTEGVQLTQSAAGDNFIRLALVPPGDAIAEPVKDTIVVCDRPTKTEPRSTGGAFEYDL
                     SPRIPLVVKHEIKFTKIRGFPVVRRAVTLQPTAPPLKQDVILMVGNNIALPGAKHSVF
                     TPRYDGRGDVIENEPGRQWVWRLSGQALPRQGSPEVLAIPLVSESAGDKTPRLTHMAD
                     PFFSTGFLLADSATSREGEFNCVFLGSRVPMTEPEQRLFWTVIQDGGPEKALQSWYAI
                     PLSDVKEGPDWLHDVAWQHYDYLSHGGKGWFDDIDAAEKLIPKAERSKIVFTLHGWYD
                     MLGRYTFDEKAGKLDDEWTAFPKAAEMKDKGFPTLEPVKMTKAEMHRRIRYAKEKGFR
                     VCVYFADGLTACDGAGFNKAEEILIPGGWVGPDTVGEPYIQNPVHPKVCQRYVSYLKA
                     FLAEYGREIDALVWDETFQIRAGTLAAGETRDYPDRAVMKLVRDLTQLVSTTQPKVAF
                     LVSDCQGMSFDGKNHWLDVPGYAMMAHGCYQDSHCEPVTWPYGIFANFRNVLWSCNWQ
                     AVTRFDYTVFGVEYYGVPIATSNGWLDDKGIAGLSESDLAAVLKLYEKRKGRRQELKW
                     LTGPAPKMKK"
     gene            complement(5766..7358)
                     /locus_tag="P5316_14670"
     CDS             complement(5766..7358)
                     /locus_tag="P5316_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HRR86248.1"
                     /translation="MARQMGLEKSGHSCGCSCCSSGLLAAIEAAKKVGLSRRNLLVGA
                     GSVAVGSWAVAARSAHAQQAAAGGEPKFVPAKELIVQPVLTYITPQRQEQTSWRPWGG
                     IQTQAQADEEASRIEKELRQMAESAGLAIRFLPVAKVADRDQASNVAKTKCDVMLIYA
                     AGGAPDWLDMLTGKDRPTLYFLRHRSGPVSLYYEILHPHFLRKATDEYADTGIDVNDV
                     VVDEYDDLAWRMRALLALRKTLGTRIIAVGGPGGWGAGRNLAPPIAREKWSLDIRTIE
                     YPDVAKRLESLRKDDKVVAEARKQAAEYLAKPDTVLKCDKKYVENAFVLYKAFKDMMA
                     EHDCAAFTIHHCMGTIMPISQTTACLPLSLLNDEGYLAFCESDFVVIPSGILMHNITG
                     LPVFLNDPTWPHHGAVTIAHCTAPRRMDGKNDEPVEIHTHFESDYGAAPKVAMKVGQV
                     VTNVVPDFECKKWVGALGTVEANPFHDICRSQSDIAIKGDWEKLLKDMRGFHWMTGYT
                     DCMKEIGYAIKHLGIAWEDVSA"
     gene            complement(7465..8889)
                     /locus_tag="P5316_14675"
     CDS             complement(7465..8889)
                     /locus_tag="P5316_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86249.1"
                     /translation="MSMDLSRREILGAAAVAGVVAVGCSRQSQRISRDVEAAEAVTIT
                     RRPRTRIRKVYLAKPVPTWPKPTLDVQAEMKRIDDILAGMADRMGGIQFEGGDLYRVP
                     EEVPGTAEALGHPDGLLVFNLTSTVGQHISKLTATGLPTIMFSQPYSGHDWSVVASLQ
                     KAGHRVICLSTSDFNEIPDALRLVRTVHLLRDTRIVHLSTGPYPAELTARIKEQLGPT
                     VVSITPERLDMAYRAVGDQAAQAEAQEWIRGAVKVIEPTEAELVASGRLYLALRAIMN
                     EEHAQAFTINCLGMFHQKRLPAYPCLAFAKLNDIGLTGVCEADVDSTLTQIMLTYAFG
                     VPGFVSDPVIDTAKNTVIHAHCVSATRMDGPTGPRAPYIIRSHMEDNKGASLQVKMRL
                     QQAITCAKLVDLDTMLVSTGTIVDNPDCDRGCRTKITTTVADARKMLNNYGGGLHRVI
                     VYGDRVNAIKDLATLLKFKVVEEC"
     gene            9079..12186
                     /locus_tag="P5316_14680"
     CDS             9079..12186
                     /locus_tag="P5316_14680"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRR86250.1"
                     /translation="MPASSSNLVVVITVLICCCLLRASDVQATDMSEAFALFEKPRRE
                     YATAPLWVWNDMLTEEQVVGTMRDLAAQKVMQVFVHPRPGLMTPYLSNEWFQLWRAAL
                     AEAEKLDMNVWIYDENSYPSGFAGGFVPEAMPESRGRGLEMKESVAPPSWGADTVAVY
                     RLDGEKYENVSRQIQANETLSPGKYVACQVRRAPDQAWTGNRCYVDLLYPGVVEKFLD
                     VTAERYRREIGQHFGKRVPGIFTDEPHLQPAGGLPWTERLPEAFEKRWGYSLLDHLIC
                     LSKPLGDYKLVRHNYLQLLHELFVECWGKPSFEFCRRNNIEFTGHYWEHEWPYCLRVP
                     DSMAMYAWHQRPAIDVLMNQYNQDSPQAQFGNVRIVKELAGVANQLGRRRTLCEAYGA
                     GGWDLRFEDMKRIGDWLYVLGVNTLDEHLSYISIRGARKHDHPQSFSYHEPWWDAYHV
                     SAGYFARLSAAMSQGEQINTIALLEPTTTAWMYNCGPDGVAMMGELGNSFQKLVTTLA
                     LAQVDYDILCEDILARHGAAEGKGLVVGRRKYEVLVLPPMTENLNAATVKLLGDYLKS
                     GGQVLSCGEPPARVEGKESLRVAEMARSGNWKQVTADELPRLLPGMSKDGFAVRQAAG
                     DRGILFHHRRQLNGGELVFLVNTSLDSSCSAKVESACASAEKWDIEQGKVEQVAYEQT
                     DKGIRIPVELAPGGSLLLALHKDRRIGPAPPHGSASRDEVRPGGEVKIRRVDPNVLTI
                     DYVDVTTNNRTRENINVLKAANFVFAENGLKKNPWDRAVQFEDELIRHRFSSSSGLEA
                     AYRFVIREKVPESLCIVIERADLYKITCNGKEVKAAEGQWWLDKAFGKIDIAATAQMG
                     ENRVVIKASPLTMFHELAAAYVLGDFNLEPAEKGFVIVPAKDLKLGPWNTQGLPLYSH
                     SVAYTRSFDVGSKNGRYYVSLPAWLGSVAAVKVNGQLAGYVWHQPWELDVTDVLKQYG
                     NDIEVVVIGTLKNTLGPHHGNPGLGSAWPGMWDNAPEQGPPPGAEYSTVGYGLFEPFV
                     LKRGS"
BASE COUNT         2324 a         3766 c         4055 g         2407 t
ORIGIN      
        1 tgaagaccaa ccccctccgg ccgaaagcca ggagccaaca cctcaagaac tctctccgac
       61 caaacgaacc caccgagcaa gtagggtccg acctgcccta cctgctttgc cggcgacgga
      121 aggcctccac cttcgcgtca ttgagaaccg agtgagtgac aacaagggca aaaaggctgt
      181 aaatccacgg cggcaggttc ctgatttcca gtggatcgcc ggggaaggaa gccctcgaat
      241 gtggcggaaa caccagcttg tctggcgcgt agaatgtccg gacaagggga ttctcctgga
      301 tatgaaacac gcggggagac cccaggccgt gtcccagacc gccgtcccag accagaaaac
      361 caaggtcgcg gctgacgaag aaagccatgc cctctggcgg ggcaggtgta ccggcttatc
      421 cgcgtcacat tattcattgc atgcaccatt acgttcctgg ggtcagcgaa acgggttgaa
      481 aaccgtcaat tctcggctgc ttgtcggatc accgcgcggg tcttgtcgag cgatgtccga
      541 gcgacgagga ggggatcctg gcggtagtag tcgggattga acagctcgat ggagagcgaa
      601 ccggtgtagc cgatgcggcg gaggtcgcgg acaagctgtt tcagcggggc gatgccgtcg
      661 ccagggtgga cgcggtgctt gtcggcgatc gactcttgcg gcgggttggc cgggtaatcg
      721 ttgagatgaa aaccagccaa caaacgcggg ttgaggtgcg cgacgtcggc caagtccgat
      781 ccgccgcggt agagatggaa aacgtcgggc aggatgcagg ccttggggtg gtgggcctcg
      841 atggcgatca ggacggcctg tccgagccgg tggatggccg gcgatgctcc ccagatctcc
      901 acggcgggga taacgccgaa cggctcggcc agttccaata gcgcccgata ccgctcggcg
      961 gccttgagca gatcgacgcc tttgacatcg ccggccggcg gggcggctat gtgagtcgcg
     1021 ccgatctggg caagttgctc catccgacgc ttcgcctcat cgaagccttt ggcccgccgg
     1081 gcgtcgtcgt ccgccatcca ctcaaagaac gcgattgccc cggtgacctt aaggccgtga
     1141 tccttgatcc gcttgttcag gtccgacagc gtgccgccat cggcgacgtg ccgatcgagt
     1201 tcatccaccc acggttcgat gccctggtaa ccagcctcgg cggcgatgtc gatcacgcgg
     1261 gcgatgggca gcttgtgacc gcggagcgtg ctggtgttga ggctgatgat gaagtcgcca
     1321 tagggtttgc tggacggcgt ctgcgcatcg gctcgcgcgg cgcggaggcc aacggccgtc
     1381 gccagaccgc cggcaagcaa ttcacgtcga gtcagattcg cgggcaagag agttctcccc
     1441 gggtctaccg aggggatgaa cccctcggct cgtcgccggc cccggaatcc ttcctggggc
     1501 ctcttgggtc gcggattccc gtcctcaccg gcaccgaacg cggaacggat tccgccaaga
     1561 gaggcggccc gaaaaggatt ccgggctgag cgacggtggc aatcatcgcg agccaccaaa
     1621 aagaagctta tgccgtatat tgcagccttc ctccaccaca tcaaatccgg tgaaccgtgc
     1681 gaggcggcgg gtgtcggcca tgtggttgcc gtagaagatc acgcggtgca ggccgttgct
     1741 ccactgttcg aacatcttgc gggcgtcggc gaccttgacg gcgatcttgg tacgacaggc
     1801 ccggtcgacg tcgggcactg cgacgatctt gccggtggag accagcatcg caccggtgcc
     1861 gtagcactcc atcgggctgg cggccagcat cggcttttcc accggggcgt acgggtcggc
     1921 cttaaccagc ttggccatgg tgatctcctg gccgacgcgc atggcgacct gcaacaccgc
     1981 tcccttatga tcctcggtgt ggctgcggat gatgtacggt gcggcctcgc cctggggacc
     2041 gtccatcttc gtcgccgaga cgcagtgggc gtggatcacg gtgttggttg cggtgtcaaa
     2101 gaccgggtcg gtgacgaagc cgggtttgcc ggtcaggtgc aggtagatga tctgggtcat
     2161 ggtcgagggc aggtcggcct cgcacacacc caccaggccg aggtcattaa gtctggcgaa
     2221 cccgaagcac gggtaggccg gcagtccctt ctgtccgaac aggccgaggc agttgatggt
     2281 gatggcctgg gccttcgagt cggccagcac cttctggagc gcgaggttca tgcgggcgct
     2341 cttggtgatc tcatccttcg acggctcgcg aatctccttg gcgttcttga tccactggtc
     2401 ggcgtcggcg ctgaccgcgg catcgtccat cgcgttatag acctcaacca gtttcctgtg
     2461 atcgaagtcg atgatctcgg tgccgaactt gtccttgacc tctttgacgt agtcggccgg
     2521 agcaggccgt cctccgctga cgaacaggac ccgcgtttcc ttgaggcgat ggatcgcacg
     2581 gaaagggcgg atggcatcgg cggcgtcggc gaagttgctg ctggggatga catcgatctt
     2641 cttgccggcc cgctggaggt cgggcacgat gcaccattcg tggccgctgt agggggtggc
     2701 gtagagcacg gttggtcggc cacagtcggc caagcggttg accagatggc tggtgccgat
     2761 gcacacgatc acacccagta tgccgtcaac gtccttgtgc tgctccagca ccttgtcgag
     2821 cgattcgacg ttctggatca gcgcattgga gaggaactcg acgtcctcca ttccggggac
     2881 tttgtcgatc tcctgctgca gtcgtttggc ttcggcgggg atgtccaggt ccggccgtgg
     2941 ccagcccgag tgtgtcccgc cgatgaagac gcgcaggacc ctggtcttgg tgtagccggc
     3001 tgccgggccg gccgcctcgc cgcccgccgc ccaagacatg cgaccaacgg ccgacgcggc
     3061 caccaggccc gccgacgctc cgatcaactc ccgcctgctc atgttcgtgt tcatcgtctc
     3121 ctccagatca acgcgcagaa ggcccatgat gaccccgccg cgacaaggcg gaaagggtgc
     3181 tcgagaatcg aactgacagt atagcagccg gcgggcgaga caccaaggcg aatgagcatg
     3241 cctcgctgaa cgtcgaacgc tgcgttacaa gccgccccgg taggggcgaa ggtgtttagc
     3301 caggggcgcg agcccctggg attcatgtcg cgttgattca tgagccccag cggggcgaca
     3361 ggtgctcctg cacagatcag gccttctgcc gccccttcgg ggctgatctg ttttggcggt
     3421 gtctgtccag gggcttgcgc ccttggctac atacctgtgc cccttcaggg cacattggcc
     3481 ggtgtgccga ttgtcaggat ctttgggaca gcgaggccgt tgacacccag gaaacacgca
     3541 aggggtgcat ggcgggggga ggcgaggcac ccccaacatc aatcgacgcg atcttgcaga
     3601 atagaaacag gtttctattc tgcaagacct gcttccgcga agactgacaa ctgaacactg
     3661 acgactgatg acttcccctg gccttgccat ttcgcccgtt cgcggttttc atattcgccc
     3721 ctgcatcatc agaggaggaa cgcatgcggc gatatgtact gttttgtctg ttcggcctga
     3781 tgctgaccgg atcaattcac gcggaagacc tggtcttcgt tcaggatggt tggaagctga
     3841 aggtttcgga tctcggcaat attgtcgggc tggccaatac ggagggtgtc cagttgaccc
     3901 aatcggcggc gggggacaac ttcattcgtc tggccctggt cccgcccgga gacgcgatcg
     3961 ccgagccggt caaggacacc atcgtcgtct gcgaccggcc gaccaagacg gaaccgcgtt
     4021 cgaccggcgg cgcttttgag tacgatctct cgccacggat tcctcttgtc gtcaaacacg
     4081 aaatcaaatt caccaagatc agaggctttc cggtggtccg gcgagcagta acccttcagc
     4141 cgaccgcgcc gccgctgaag caggatgtca tcctgatggt cggcaacaac atcgccttgc
     4201 cgggagcgaa gcattcggtg ttcacgccgc gatacgacgg ccggggcgat gtgatcgaaa
     4261 acgagcctgg ccgtcagtgg gtctggcggc tcagcgggca ggccctgccg cgccagggct
     4321 ccccggaagt cctggcgata cctctggtca gcgaatcggc gggggacaaa acgccgcgcc
     4381 tgacacacat ggccgacccg ttcttcagca cgggcttcct cctggccgac agcgccacct
     4441 ctcgcgaggg cgagttcaat tgcgtctttc tgggcagcag agtgccgatg accgaaccag
     4501 agcagcggct cttctggacg gtgattcagg acggcggtcc cgagaaggct ctgcaatcat
     4561 ggtacgccat cccgctgtcc gatgtgaaag aggggccgga ctggttgcac gacgtggctt
     4621 ggcagcacta tgactatctc agtcacggcg gcaagggctg gttcgacgac attgacgcgg
     4681 ccgagaagct catccccaag gccgagcgat caaagatcgt cttcacgctg cacggctggt
     4741 acgacatgct tggtcgctac accttcgacg agaaggccgg caagctggat gacgagtgga
     4801 cggccttccc caaggccgcc gagatgaagg acaagggctt cccgacgctc gaacccgtga
     4861 agatgaccaa ggccgagatg caccggcgga tcaggtacgc caaagagaaa ggattcaggg
     4921 tctgcgtgta tttcgccgat ggcctgacgg cttgtgacgg ggccggattc aacaaggccg
     4981 aggagatact gattccgggc ggctgggtcg ggccggacac ggtcggcgag ccgtacatcc
     5041 agaaccccgt tcatccgaag gtctgtcagc gatatgtgag ctacctgaag gcttttctgg
     5101 ccgagtatgg ccgtgagatc gacgcattgg tctgggatga gaccttccag atccgagcgg
     5161 ggacactcgc agcgggggag actcgcgact accccgaccg cgccgtgatg aaactggtcc
     5221 gcgatctgac gcagctcgtc tcgaccactc aacccaaggt cgcgtttctg gtcagcgatt
     5281 gccagggaat gagcttcgac ggcaagaacc actggctcga cgtgcccgga tacgcgatga
     5341 tggcccatgg ctgctaccag gacagccact gcgagcccgt gacctggcct tatggcatct
     5401 tcgccaactt ccgcaatgtg ttgtggtcgt gtaactggca agcagtgaca cgcttcgatt
     5461 acacggtctt cggcgtcgag tattacggag taccgatcgc cacctccaac ggctggctcg
     5521 atgacaaggg catcgccgga ctgagcgaga gcgacctggc ggcggtgctc aaactctatg
     5581 agaagcgaaa gggccggcgg caggaactca agtggctgac cggcccggcg ccgaagatga
     5641 agaagtaggc tgacaggctt ctagactgtt agacccttag gctgttagcc tactaggcat
     5701 tcccgaaaat ggatcctcgt gaaagcctag taaccctaac agcccaacag tctgacagac
     5761 tcttcttacg ccgacacgtc ctcccaggcg atgcccaggt gcttgatggc gtagccgatt
     5821 tccttcatgc agtcggtgta gccggtcatc cagtggaagc cgcgcatatc cttgagcagc
     5881 ttttcccaat cgcccttgat cgcaatgtca ctctgcgagc ggcagatgtc gtggaacgga
     5941 ttggcttcca ctgtcccgag ggcgcccacc cacttcttgc attcgaagtc gggcacaacg
     6001 ttggtaacga cctgaccgac cttcatggcc accttcggag cggcgccgta gtcggactcg
     6061 aagtgggtgt gaatctcgac cggctcgtcg ttcttgccgt ccatcctgcg cggggcggtg
     6121 cagtgagcga tggtgacggc gccgtggtgc ggccatgtgg ggtcgttgag gaagaccggc
     6181 aggccggtga tgttgtgcat gaggatgccg gaggggatga ccacgaagtc ggactcgcaa
     6241 aacgccaggt acccctcatc gttaagcaga ctcaggggca ggcaggcggt ggtctgggag
     6301 atgggcatga tcgtgcccat gcagtggtgg atagtgaagg cggcgcaatc gtgctcggcc
     6361 atcatgtcct tgaaagcctt gtagaggaca aacgcattct cgacgtattt cttgtcgcat
     6421 ttgagcacgg tgtcgggttt ggccaggtac tcggcggcct gcttgcgggc ctcggcgacg
     6481 accttgtcgt ccttcctcag ggattccagc cgcttggcaa cgtcagggta ttcgatggtg
     6541 cggatgtcca agctccattt ttcccgggcg atcggggggg ccaggttgcg cccggcaccc
     6601 caaccgccgg ggccgccgac ggctatgatg cgcgttccga gcgtcttgcg cagggccagc
     6661 agagcccgca tgcgccacgc cagatcgtcg tactcatcga cgaccacgtc gttgacgtcg
     6721 atgcccgtgt cggcgtactc atcggtcgct ttgcgcagga aatgggggtg aagaatctcg
     6781 tagtacaggg acaccggtcc ggagcgatgt cgcaggaaat acagcgtcgg acgatctttg
     6841 ccggtgagca tgtccagcca atcgggtgcg ccgccggcgg cgtagatgag catgacgtcg
     6901 cacttggtct tggcgacatt gctcgcctgg tcgcggtcgg cgaccttggc gacgggcagg
     6961 aacctgatcg ccaacccggc cgactcggcc atttggcgaa gttctttctc gattcgcgag
     7021 gcttcctcgt cagcctgggc ctgagtctgg atcccgcccc acggtcgcca actggtctgc
     7081 tcctgccgtt gaggagtgat ataggtcaac accggctgga caatcagttc cttggctggg
     7141 acgaacttcg gctcgcctcc cgcggcggcc tgctgggcgt gggccgaccg ggcggcgacg
     7201 gcccagcttc ccacggccac ggaacctgcg cccaccaaca ggttacggcg agagagtccc
     7261 accttcttgg cggcctcgat tgccgccagc aaacccgatg aacagcacga gcaaccgcaa
     7321 gaatgcccgc tcttctccag acccatctgc cttgccattc gattaacctc cttatcggtc
     7381 gtcttgacgc ccgcaggaca ggctttcggt ccgtccctgg accggccggg aaccggtccc
     7441 gcactggccg tcagaaccga cccttcaaca ctcctcgacg accttgaact tgagcagggt
     7501 cgccagatcc ttaatcgcat tgacgcgatc gccgtaaacg ataacgcggt gcagtccacc
     7561 gccgtagttg ttcagcatct ttcttgcatc ggccacggtc gtggtgatct tggtccggca
     7621 accgcggtcg caatcggggt tgtcaacgat cgtgcccgtc gagaccagca tggtatcaag
     7681 atcaaccagc ttcgcacagg tgatcgcctg ttgcagccgc atcttgacct ggagcgacgc
     7741 ccctttgttg tcctccatgt ggctgcggat gatgtacggg gcccgtgggc cggtcggtcc
     7801 gtccatgcgg gtggccgaga cacagtgagc atgaatgacg gtattcttcg ccgtgtcgat
     7861 gacagggtcg ctgacaaacc cggggacgcc gaaggcgtag gtcagcatga tctgggtgag
     7921 agtcgagtcc acgtccgcct cacagacgcc cgtcagcccg atgtcgttga gcttggcgaa
     7981 cgccaggcaa ggataggccg gcagccgttt ctggtggaac attccgaggc agttgatggt
     8041 gaaggcctgg gcgtgctcct cattcatgat cgcccgcaag gccaggtata accgtccgga
     8101 cgccacgagc tcggcctccg tcggctcgat gaccttgacc gcgccacgaa tccattcctg
     8161 agcttcggct tgggcggcct ggtcgccgac ggcgcgatag gccatgtcga ggcgttccgg
     8221 agtaatagac acgaccgtcg gcccgagttg ctctttgatc cgtgcggtca gctcggcggg
     8281 atacggaccg gtgctcaggt gtacgattcg ggtatcacga agcaggtgaa ccgtccggac
     8341 gagccgcaag gcgtccggga tctcgttgaa gtcgctggtc gacaggcaaa tcacgcggtg
     8401 accggccttc tgcaatgaag caacgaccga ccagtcgtgt ccgctgtagg gctgggagaa
     8461 catgatggtc ggcaggcccg tggcggtaag cttgcttatg tgctggccta cggtcgaagt
     8521 gaggttgaac accaacagac cgtcggggtg tccgagagct tcggcggtac ccggcacttc
     8581 ctcgggcacg cggtagaggt caccgccctc gaattgaata ccgcccatcc ggtccgccat
     8641 gccggccagg atgtcgtcga tgcgtttcat ttccgcttgc acgtcgagtg tgggcttcgg
     8701 ccatgtcggc acgggcttgg cgagataaac cttacggatg cgcgtccgcg gccgtctggt
     8761 tatcgtcacc gcctcggcgg cttcaacatc gcggctgatt cgttgagatt gccggctgca
     8821 cccgacggcc acgacgccgg cgactgccgc cgcaccgaga atctctcttc ggctcagatc
     8881 catgctcatc gctcgttacc tctctaaagg gacgacaata ctcgacttgg cggcctattg
     8941 tccgccaaca ggggagcgat ttcaaccttt ggcgtgtgtt tcttgttcgt tcttctccgg
     9001 acggttagca tgtcccgttg accagtccgc agggccggag ccggtctcaa ttggggcgca
     9061 cacacgagga gccgtcacat gcctgcctct tcaagcaatt tggtcgtcgt cataaccgta
     9121 ctgatttgct gctgtttgct gcgggcttca gacgtccagg caactgacat gtccgaggcc
     9181 ttcgcgttgt ttgagaaacc gcggcgtgaa tatgccaccg cgccgctgtg ggtgtggaat
     9241 gacatgctga ccgaggagca ggttgtcggc acgatgcgag atctggcggc ccagaaagtg
     9301 atgcaggttt ttgttcaccc gcggccgggt ctgatgactc cgtacctgtc aaacgaatgg
     9361 ttccagcttt ggcgggcggc cctggcggag gctgagaagc tggacatgaa cgtctggatc
     9421 tacgacgaga actcctatcc gtcgggtttt gcaggcgggt tcgtgccgga ggcgatgccg
     9481 gaatcgcgcg gacgcggcct tgagatgaaa gagtccgttg cgccgccgtc ctggggcgcg
     9541 gacacagttg cggtctaccg gctggatggc gagaagtacg agaacgtgag ccggcagatc
     9601 caagcgaacg agacgctttc ccccggcaaa tatgttgcct gccaggtgcg acgggccccg
     9661 gaccaggctt ggacgggcaa tcggtgttac gtcgatctgt tgtaccccgg cgtggtcgag
     9721 aagttcctgg acgtgacagc cgagcgttat cgacgggaga tcggccagca cttcggcaag
     9781 cgtgtgcccg gcatcttcac tgatgagccg cacctccagc cggcgggtgg gcttccgtgg
     9841 acggaacgtc tgcccgaagc attcgagaag cgatggggct actcgctgct cgatcatctg
     9901 atctgcctga gcaagccttt gggcgactac aagctggttc ggcacaacta cctgcagctt
     9961 ctccacgagc tgttcgtcga atgctggggt aagccctcgt tcgagttctg ccggcggaac
    10021 aacatcgagt tcaccggcca ttactgggaa cacgagtggc cgtactgcct tcgcgtgccc
    10081 gacagcatgg ccatgtatgc ctggcaccag cgcccggcca tcgacgtgct gatgaaccag
    10141 tacaaccagg acagcccgca ggcccagttc ggcaatgtcc gcatcgtcaa ggaactggcc
    10201 ggcgtcgcca accagttggg ccgcaggcgc acgctctgcg aggcctacgg ggccggcggc
    10261 tgggatttgc gattcgagga catgaagcgg atcggcgact ggctgtacgt gctcggcgtc
    10321 aacactctgg atgagcacct gtcgtatatc agcattcgcg gggcccgcaa gcacgaccac
    10381 ccgcagtcgt tttcgtacca cgagccgtgg tgggatgcct accacgtatc ggccgggtac
    10441 ttcgcccgcc tgtcggcggc gatgtcgcag ggcgagcaga tcaatacgat cgcgctgctt
    10501 gagccgacga ccaccgcctg gatgtacaac tgcgggccgg acggcgttgc gatgatggga
    10561 gagctcggca acagcttcca gaagctcgtg acgacgctgg ccctggccca ggtggactat
    10621 gacatccttt gcgaagacat tctggcacgg catggtgcgg cggaaggcaa gggtcttgtc
    10681 gtcggcaggc ggaagtatga ggttcttgtt ctgccgccca tgacggagaa cctgaacgcg
    10741 gcgacggtca agctgctcgg cgattatctc aagagcggcg gtcaagtgtt gtcgtgcggc
    10801 gagccgcccg cccgggtcga aggcaaggaa tcgctgcggg tcgccgagat ggcccgatcg
    10861 ggcaactgga agcaggtcac ggctgatgaa ctgcctcgcc tgctgccggg tatgagcaag
    10921 gacggcttcg cggttcggca ggcggccggt gatcggggca tcttgttcca tcaccgccgg
    10981 caattgaacg gcggcgaatt ggtgttcctg gtcaatacca gcctggattc gtcgtgctcg
    11041 gcgaaggtcg agtcggcctg cgcgtccgcg gagaagtggg acatcgagca gggcaaggtt
    11101 gagcaggttg cttatgaaca gacggacaaa ggcattcgca ttcccgtcga actggccccg
    11161 ggcggcagcc tgctgctggc cctgcataag gatcgccgca tcggccccgc gccgccgcat
    11221 ggttcggctt ctcgcgacga ggtcaggccc ggcggcgagg tgaagatccg ccgggtggat
    11281 ccgaacgtgc tgaccatcga ctacgtggac gtcaccacca acaatcgaac gcgagagaac
    11341 atcaacgttc ttaaggcggc gaacttcgtg tttgccgaga acggcctcaa gaagaacccg
    11401 tgggaccggg cagtgcaatt cgaggatgaa ctcatccgcc atcgcttctc gtcgagcagc
    11461 gggctggagg cggcctatcg cttcgtcatc cgcgagaagg tacccgagtc attgtgtatc
    11521 gtgatcgagc gggcggacct gtacaagatc acgtgcaatg gcaaggaggt caaggcggcc
    11581 gaagggcagt ggtggctgga caaggccttc ggcaagatcg atattgccgc caccgcccag
    11641 atgggcgaga accgcgtggt gatcaaagcc tcgccgttga ccatgttcca cgaactggcg
    11701 gccgcatacg tgctgggcga tttcaacctt gagccggccg agaagggctt tgtgatcgtc
    11761 ccggcgaagg acctcaagct cggcccgtgg aacacgcagg gattgccgct gtattcgcac
    11821 agtgtggcgt atacgcggtc ctttgacgtc gggtcgaaga acggccgtta ctacgtctcg
    11881 ctgcctgcat ggctgggcag cgtcgcggcg gtgaaggtca acggccaact tgccggttat
    11941 gtctggcacc agccgtggga actcgatgtc actgacgtcc tgaagcaata cggcaacgac
    12001 atcgaggtcg tggtgatcgg cacgctcaag aatactctgg gcccgcacca tggcaatccc
    12061 ggcctcggct cggcctggcc gggaatgtgg gacaacgccc ccgaacaggg cccgccgccg
    12121 ggtgcggagt attccacagt tggctacggg ctgttcgaac cattcgttct gaagcgcggg
    12181 tcgtgaggag acgccgcgtg tcgacgggga agaccgaggc agcgcaacgg cggcgctggc
    12241 atagctgtca ggcaaaggcg ggcgggatgc gaacccaggt tcagagaacc taggccaccc
    12301 gagtggtgcc gacgccctcg tcggcactct ttcctgccac ggttgcccca tctcttgagc
    12361 gctggagcct tcaacttcag gcagagattc gctcccccgc ctcgaaacga ggtgggccac
    12421 ccgagtggtg ccgacgccct cgtcggcact ctttcctgct acggttgccc catctcttga
    12481 gcgctggggc cttcaatttc aggcagagat tcgctccccc gcctcgaaac gaggtgggct
    12541 gcccgagtgg tg
//
LOCUS       DAOTPW010000167        12513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4831_length_12513_cov_6.507947, whole genome shotgun sequence.
ACCESSION   DAOTPW010000167 DAOTPW010000000
VERSION     DAOTPW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12513
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4831_length_12513_cov_6.507947"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            464..1072
                     /gene="trmB"
                     /locus_tag="P5316_14685"
     CDS             464..1072
                     /gene="trmB"
                     /locus_tag="P5316_14685"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRR86251.1"
                     /translation="MLTIDDVLLPPPGIGELIDFQAIFGDNRPIEMEIGTGKGGFLLN
                     RARALPDRGFLGIEWADKICRYAADRMVRWGVTNVRLMRADAGHLVAHSLPAKCLTIL
                     HIYHPDPWPKKRHHKRRLIQPAFIAAAANALLPGGRIAIQTDHAEYFEHIRQVVAGEP
                     RLIEVPFDVPEAGVQEGRVSTNYEIKYLREGRPIYQLAMMKK"
     gene            complement(1168..2355)
                     /locus_tag="P5316_14690"
     CDS             complement(1168..2355)
                     /locus_tag="P5316_14690"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HRR86252.1"
                     /translation="MTILAVAFVIAVSPSASPVQALMLDGQVFEGQWAGPGQEGEIRL
                     QTGGAARTVRIDELLSLRWQTAHTAAASQSQPLPLVIHLRDGGRISARVLGSDSGGLE
                     LETKWTKSLIVPLSLMAAMCSCGDQRHDLSAAFEQTLADKKPDEDQLLVVRNNRVTTL
                     KGTLESLGADGGSFRWRDRSVPFAAQEAFAVVMATGQSTPPLAQALCSLRDGCTWAGQ
                     IVGIEADLLRLRLSMGTVLSLPIEQMSEIRFRSDRVLFLSDLTPAKYEFEPFGATRWP
                     YRVNRAVSNQPMRIGEQSFDRGIGVHSRSVLTYDVPEGFVRLAAMIGIDEAVGDRGNV
                     IFRVLADGKEVFNSGPVTGRDAARPLLATIENARQIQLIVEFGDDIDIGDQADWAEVR
                     LIR"
     gene            complement(2464..3156)
                     /locus_tag="P5316_14695"
     CDS             complement(2464..3156)
                     /locus_tag="P5316_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86253.1"
                     /translation="MNTEATGIPFAERFRVHGSGFGMDRGRELPNPGQRFVIRCLSFR
                     AIMSFALLWHGSFGVAAPASAPETAPSGCGPPSVATRPSDDLSSVWALMSQAGQRLDA
                     GKADVVTRQSQRQAVEILDRLIKQVEEQERQQRQGQCKACGGKGCRLCRRSTAAAVRR
                     PNQPAKESTASPSQGGPGEQHSPATARPGETWGNMRPEERERILQSIGKDFPTQYRQL
                     VEQYYKQLAKEE"
     gene            complement(3153..4517)
                     /locus_tag="P5316_14700"
     CDS             complement(3153..4517)
                     /locus_tag="P5316_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86254.1"
                     /translation="MFCILLPVAPAAAAEPLSTVQTDATASPLATKQQIVRDRMIQLE
                     DRMFRLIEQLSQREPEQAARLEAALKKARELLIRRDMEVTIRLLSDEDLAGASDKQIE
                     IVRDLESVLKVLLEEPDGSRQRQEEIARLEALKEEVRRLLEAQQRLEAIAEAAEPQRQ
                     LGEKTGQLGERMSGRKTETQPARPPDPGNEHVARGAQNMMRASQELGQQKTEQGAESQ
                     REAEDELRQAIENLEQAIEQLKQEQRDQTLRQLQVLLQAMLDKQLLINRDTQVLDEKG
                     RAQWTHADELALTGLSKDEMTLADQTSQMLRLLKADGTTVVFPQVMGQVRDDMERVAA
                     KLQKKDTGTDTQRIEVAIAETLSQLIDSLKQAAANPPPGGGAGGGGGQGCNPPLVAAS
                     AELKLLRGCQQRLNERTAMAGEAIEKNPAAGSGLRDEIRRLSTQQNELAELARQMDER
                     MKKP"
     gene            complement(4738..6906)
                     /locus_tag="P5316_14705"
     CDS             complement(4738..6906)
                     /locus_tag="P5316_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86255.1"
                     /translation="MLVNVSESARLQFRQHTLVRLDALARRIRLYLLADGLAVVSPAV
                     LAAVLITLAVDHTFRLDTSMRWVQAISVWGVLCWLSRRYLIRPAAVILAQADLATIIE
                     RKHPELGGRLIAATEFVAHPQGVNRGSAALIDKVIEQANGAASQLRFSDSLNHRRAVR
                     QATLGLTGLVGIAGLLLAAPGTMGLWFERNVLLRSVEWPLKNRLVVEGLTEGKLVVPR
                     GDDATISAAVVPGYEAPRQAYIHYHGAGSLRGQAQMPEVRGQPVRFIHMFEHLTETVT
                     CRIVGGDAQTDPFTIEVIDRPSITRARIGIAPPAYTRLEPYELREGQNVAEALVGSEI
                     RLTIEASQPIASAILVRDVGGKQSTVGPAECVGEREFRASDRPTTSAAYHFELTSPSG
                     LTNVSPRVPLTRFVVRVDPDQAPVVKMRIQGAGEMITPQAVLPTDMEFSDTYGLASVS
                     VVFSSGKSEDKGIAEPLAGFEQGSKIFNRSVEWSVAEHGLAEGDRLSIWAEAADFDDI
                     SGPNVGKSTPAVFRIVSREELAADLNRRQHEYRQDFERHLRRQEELYSELLSVLEPAT
                     AAADRRQHSQRLRQLARRQRDQAGQIATVARQFEQVLAEMRVNALSSATVEERLGRGV
                     AQPLRGLSREQMPAAAEMIDRLAGGDDAEWVRKARDAQSTLRDEMNNILARMLEWEKL
                     EEAVILLRDVLNMQRNVGQETETKLERNILGTMPGSEPAK"
     gene            complement(6920..9349)
                     /locus_tag="P5316_14710"
     CDS             complement(6920..9349)
                     /locus_tag="P5316_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRR86256.1"
                     /translation="MRLLAETATETLERRLEFAGLPQGWHAIVAMVLLELILWASVHL
                     YRREQRVGASARIRAILALLRCTLLAVLALIWLQPVIATYSRREMEAPTLLLIDGSAS
                     MSLHDRYADETEQRRVRQALGNAQVNELATLSRAEIARSVLARDDHRLIRELVRNNPV
                     LCYRFGDGIDLVGRYTAAAGSGGGRIGIAVPASAPATDIGAAVRQAVESQGGRPVSAV
                     VVVSDGQFNKGEAVDAVAGYARAKRIAIHTVGVGDPAPPRNAAVAAIEAPASIFVNDP
                     FKITARIAAQGLCGQSLIIELLQRLPDASQPSVVATRTIEAGPDDHVSSITFDHQLIR
                     PLQTRLAVRIQPVEGEIIAEDNERETSVRGLEKKMRVLLVAGAPSWEYTYLSRLLTRD
                     ATVDVSCWLQSADEEAVRDGGTVIDHLPRKHEELGVYDCIILMDPRPEEIDAGWSKTL
                     ETLVSQAGTGLVFVAGRKNTSKLAREAAAKALLDLLPVVIDAGQADLIINELGYFQQT
                     AWPPAIPPQVSGHPLLNIADRSEENAQTWAQLPGVYWHYPVSREKPVATVLLRHSNPQ
                     MRNAGGQHVLLATQFVGAGRTAFMAWSDTWRWRRAGDRYFNRFWITLIRHLVEGKLLG
                     GQKRGLIQPTKDHCPVGEAVGIEARLLDSKFSPLGRDTVEMTVEAEGVPGHVVVLKAQ
                     PDRPGWYHGELTPTETGIYELRIDLPAEGGEGTTTIRGELRVGRSDLEFRRTALDRES
                     LENLAAGSAGGRSLYLDEIDRLPSLIPAKSVSMVTTGRPTPLWDRWWTLFVLVALLST
                     EWALRKAFNLL"
     gene            complement(9346..11721)
                     /locus_tag="P5316_14715"
     CDS             complement(9346..11721)
                     /locus_tag="P5316_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRR86257.1"
                     /translation="MIAGNQVLAAMITPAMFYAGLVCAAIPIVIHLINRRRFRRVRWA
                     ATQFLLEANRQNRRRIRIEELILLMLRCLAMLLTGMMLARVFVRPQAFAGLLGSRAGT
                     DYVVVFDDSFSMGLADAGSGASTQDTSVFDRALTAVERLVGWLREECPSDALTIVATS
                     RPEQTIVTETSVGRLDPIAFSRTWRDRKPSSRRADLPEAFGAVRRLLDARRSAGAVIY
                     LVSDFQRIDWLAGESAQAAGNGGPGAGGSQSDKKLGTAESPLASLAGWDRADRSLKIV
                     LVDVGSENTDNLAVTGVESRQAQAVAGTGTRLIAHVANLGRSKSRARTMQVFVGEAGL
                     PAVTVPAIPAGQSIEVPVEAVFPQVGSAALTVELQADALPVDNRRFCVVPVAKSIRVL
                     LVNGEPSSDPYQDETFLLSVALRPQGPQFSGNEIAAISEDELEQTDLASFHAVILANV
                     VRVTESAAARLESYVASGGGLAFFLGDQVDVEAYNQVLFRDGAGLLPGKLSEPVAAPD
                     ESPGFRVGEMNISHPALRPFANLMPACFEGVLVWAYFPVSITPAPLTQSSTRTPAQTA
                     ISLLQLDDADRSPLILQKDHQAGHVWLVTSSADKEWNNLANHPIFVVFAMEMTQYLAR
                     RPAQKAMQLVGQPVTLSLDAGRYQPTAAVRSPAYPEEPAVAVHAEPDTSSGGMAIRWT
                     QTDQPGFYQIDLTEQSGGHVVEPVAVNVDSTESDLRRMRRDELQQATAGWPVEYIRGE
                     ELTQGSAAATRKELWPALLILLVGTLMTEQLLACWFGANRQWSALMGRHRA"
     gene            complement(11877..>12513)
                     /locus_tag="P5316_14720"
     CDS             complement(11877..>12513)
                     /locus_tag="P5316_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRR86258.1"
                     /translation="PPLARGGWGGSRMSKFDYACTVAASLAYLLLNQQDSCGLVLFDH
                     AIRDQVPPASSPAQVSSIIELIERQRPDRTTDMKMLPAQLADQLRQRGLIILISDLLA
                     DADEIVGGLQQLRHGRQDLIVLQVLDHDELTFPFEHNTLFEGLEDPGIQLLTDPQSLR
                     AAYLRVVRDFVSRIRASCVNNRIDYRLLSTTDPLDGALTAFLAGRMRQTGR"
BASE COUNT         2280 a         4284 c         3604 g         2345 t
ORIGIN      
        1 gcccggatca cgtccacctc gaccatcttg gtgacggcca caaggcggac gctcccaggg
       61 tcccggccgc tccgccggca tgccgcgtgg atgcggtccc gaacacgttt gtagttgtcc
      121 gatagcttgc gtttcatggt gagtcgctgc aagctgcccc aacgggcgtg cttcaaagta
      181 gcgattatac tcgtggcaga ccctttgtga gactcccgct gatcccgtcg aagccgacag
      241 cggtccgcaa ccgcttggac ggggccacaa ccggcttgtc agccaacgtc ttctgcccct
      301 ccagaccgcg cggctgaaca ggccgcctgt ggcacagaaa aaacgccctc accgaattcg
      361 tcaccgactc gcccgacgac cttccctccg gtcccccgac cgtacgcgtt cctttccagc
      421 catcaggttg atccgtccag cggcgccggg gatcataatc gttatgttga cgattgacga
      481 tgtcctgttg ccgccgcccg gcatcggcga gctgatcgac ttccaggcga tctttggcga
      541 caaccggccg atcgaaatgg agatcggaac gggtaagggg ggcttcctcc tcaatcgggc
      601 ccgcgccctg ccagacaggg gcttcctcgg catcgaatgg gctgacaaga tctgccgcta
      661 cgccgctgat cgaatggtcc gatggggcgt gacgaatgtc cgtctaatgc gcgcggacgc
      721 cggtcatctg gtcgcacaca gcctgccggc caaatgcctg accattctgc acatctacca
      781 ccccgatccc tggcccaaga agcgtcacca caagcgacgg ctgattcagc cggccttcat
      841 cgctgcggcc gcgaatgccc tcctgcccgg cggccgaatc gccatccaaa ccgaccacgc
      901 cgagtacttc gagcacattc ggcaggtggt agccggcgag ccgcggctga tcgaggtccc
      961 cttcgacgtg cccgaggccg gggtgcagga gggtcgtgtc tcaacaaact acgagatcaa
     1021 gtacctgcgc gaagggcgcc ccatctatca acttgcgatg atgaagaagt gacgagggcc
     1081 tgcaatcagc cgtcatcttg catgtcagcg accccattca acaagccttc gacccgtgct
     1141 cgagagccgt ggcaagcgat ggcttttcta tctgatcagc cgcacctccg cccagtcggc
     1201 ctggtcgccg atgtcgatgt cgtcgccgaa ctcgacgatg agctggatct gccgggcgtt
     1261 ttcgatcgtt gccagcagcg gacgggcggc gtcacgaccg gtgaccggac cgctgttgaa
     1321 gacctccttg ccgtcggcca gcacgcggaa gatcacgttt ccccggtcac ccaccgcctc
     1381 atcgatgccg atcatcgccg ccagtcggac gaatccctcg ggcacgtcgt aggtcagcac
     1441 cgatcgcgaa tgcacgccga tcccgcggtc aaacgactgc tcaccgatgc gcatcggctg
     1501 attcgacacc gcgcgattca cgcgataggg ccaccgggtt gcaccaaacg gctcaaactc
     1561 gtacttggcc ggggtaagat cgctcaggaa cagcacgcgg tcggatcgaa agcggatttc
     1621 gctcatctgc tcgataggca gactcagcac agtacccatg ctcagccgca gacgcagcaa
     1681 atctgcttcg atgcccacga tctggcctgc ccatgtgcaa ccatcgcgca gcgagcagag
     1741 tgcctgagcg agaggcggcg tcgactgccc ggtcgccatg accacggcga aggcttcctg
     1801 agctgcgaat gggacggatc gatcacgcca gcggaaagag ccgccatcgg cgccgagcga
     1861 ctccaacgtt ccctttagcg tggtcacgcg attgtttctc accaccagaa gctgatcctc
     1921 gtccggcttc ttgtcggcca gggtctgctc aaatgccgcc gacagatcgt gcctctgatc
     1981 accgcaactg cacatcgccg ccatcagaga cagaggcact atcagcgact tggtccactt
     2041 tgtttccagc tcgagcccgc ccgagtcgct gcccagcacg cgtgccgaaa tacgaccacc
     2101 gtccctgaga tggatgacca gaggaagtgg ttgggattgc gaagccgccg cagtgtgcgc
     2161 tgtttgccac ctcagcgata gcaactcatc aatacgaacg gttctcgccg ctcccccggt
     2221 ttgcaggcga atctcgcctt cctgtccggg ccccgcccac tgcccctcga acacctgccc
     2281 atcgagcatc agagcctgaa ccggcgacgc cgaaggtgat accgcgataa caaatgcgac
     2341 cgccaggatg gtcattcagt caccccctac gccgtgagca ggctccggca ggatcattgt
     2401 cttccctgtg attctccatt cagtgttcgc aattcatcag cactgcgctc cgctacgccg
     2461 aggctattct tccttggcca gttgcttgta gtactgctcg acgagctgtc ggtactgagt
     2521 ggggaagtcc tttccgatcg attgcagaat gcgttcgcgt tcctcgggac gcatgttacc
     2581 ccatgtctcc cccggccgag cagtcgccgg tgaatgctgc tcgccggggc cgccttgcga
     2641 gggcgaggcc gtcgactcct tggccggctg gttgggcctg cgcacagccg cggccgtgct
     2701 cctgcggcat aggcggcacc ccttcccgcc gcacgccttg cactgccctt gcctctgctg
     2761 ccgttcctgt tcctcgacct gcttgatgag ccggtcgagg atctcaaccg cttgtcgctg
     2821 gctttgccgc gtgacgacat cagccttccc cgcatcgaga cgttgaccgg cttgagacat
     2881 cagggcccag acgctgctga gatcatccga cggccgcgtg gcgacggatg gggggccgca
     2941 accggaaggt gcggtctccg gggcggaggc cggagcggca acgccaaacg atccgtgcca
     3001 cagcaacgcg aaactcatga tcgcacgaaa tgacaggcaa cgaatgacga atcgctggcc
     3061 aggattcggc aattcccgcc cccggtccat gccaaaccct gacccatgaa ccctgaacct
     3121 ttctgcaaat gggattccgg tcgcttctgt gttcatggct tcttcatccg ttcgtccatc
     3181 tgtctggcca gctctgccag ctcattctgc tgcgtcgaca atcggcgaat ttcatcccgc
     3241 agacccgatc cagcggccgg gttcttctca atcgcctcgc ccgccatggc cgttcgctcg
     3301 ttcagccgct gctgacagcc ccgcagcagc ttcaattcgg ccgatgcagc cacgagcggg
     3361 ggattgcatc cctggccgcc gcctccgcct gctccgccgc cgggtggcgg gttcgcggcc
     3421 gcctgcttca gcgaatcaat caattggctc agcgtctcgg caatcgcgac ctcgatccgt
     3481 tgcgtgtcag tgccggtgtc tttcttctgc aacttggcgg ccacgcgttc catgtcgtcg
     3541 cgaacctgtc ccatcacttg cggaaagacc acggttgtgc cgtcggcctt gagcaaccgc
     3601 agcatctggc tcgtctgatc ggcaagagtc atctcgtcct tgctcaagcc cgtcagagcc
     3661 agttcgtccg cgtgagtcca ttgcgcgcgc cctttctcat cgagcacctg cgtatcccgg
     3721 ttgatcagca gttgcttgtc gagcatcgcc tgcagcagca cttgcagttg ccgcagcgtc
     3781 tgatcccgct gctcctgttt gagttgctcg atggcctgct ccaggttctc gatggcctgc
     3841 cgaagctcgt cctcagcctc cctctggctt tcggcgcctt gctccgtctt ttgttgcccg
     3901 agttcctgag acgcccgcat catgttttgt gccccacgtg cgacatgctc gttgccgggg
     3961 tcgggcggtc tggcaggctg ggtctcggtc ttccggccgg acatcctctc gccgagctgg
     4021 cctgtcttct caccgagttg cctctgaggt tcggcggctt ccgcgatcgc ttcaagccgc
     4081 tgctgcgcct cgagcagtcg acgcacctcc tctttcaggg cctccagccg tgcaatctct
     4141 tcctgtcgtt ggcgactgcc gtcgggttcc tcaagcagca ccttgagcac actctccagg
     4201 tcgcgcacga tctcaatctg cttgtccgac gccccggcca ggtcttcgtc gctcagcaga
     4261 cggatcgtta cctccatgtc ccgccgaatc agcagttccc gggccttctt gagagcggct
     4321 tccagcctcg cggcctgttc gggttccctt tgtgagagct gttcaatcag ccggaacatg
     4381 cggtcctcga gctgaatcat ccggtcacgg acgatctgct gcttggtggc cagggggctg
     4441 gcggttgcat cggtttggac ggtcgataga ggctcagccg ccgccgccgg cgcgacgggc
     4501 aggagaatgc agaacaacgg gaccaacagg cgcccaaatg cccaacgccc aagccccaat
     4561 gaccaatgaa atccgaatga cgaaatccga ctgacgaatg gagtccgaaa tccaaatgtc
     4621 gaagactcgc cgtggcgaga cgaaatccga gtgggccaag tagcggcggg ctctgggcgt
     4681 ttggtcattc gcacttggtc attcactggg cattggggct tgggcattgg tcattcccta
     4741 cttagccggc tcgctccccg gcatggtccc cagaatgttt cgctccagct tcgtttccgt
     4801 ttcctggccg acattgcgct gcatattgag aacgtctcgc agcagaatga ccgcctcctc
     4861 gagcttctcc cattctagca tccgggccag aatgttgttc atctcatcgc gcagggtcga
     4921 ttgggcatct cgggccttgc gaacccactc ggcatcgtca ccgcccgcca gccggtcgat
     4981 catctccgcc gcggcaggca tctgctctcg ggacagaccc cggagcggct gtgccacccc
     5041 ccgccccaat cgctcctcaa ccgtcgcgct ggacagggcg ttgactcgca tctcagccag
     5101 cacctgctca aactgcctgg ccaccgtcgc tatctggccg gcctggtccc tctggcggcg
     5161 cgccaactga cgcaaccgct ggctgtgctg ccggcgatcg gccgccgcgg tggccggttc
     5221 caggaccgac agcaactccg agtaaagttc ctcctgccgt cgcaggtggc gctcaaaatc
     5281 ctgtcgatac tcgtgctgcc ggcggttcag atccgcggcc agttcctcac ggctgacgat
     5341 gcgaaacacc gccggcgtcg atttgcccac attcggcccg gagatatcgt cgaagtccgc
     5401 cgcttccgcc cagatgctca gccggtcacc ctctgccaac ccgtgctcgg cgaccgacca
     5461 ctcaaccgag cggttgaaga tcttggagcc ctgctcaaaa ccggctaacg gctccgcaat
     5521 acccttatcc tccgacttgc ccgaactgaa gacgacggaa acgctcgcca gaccgtaggt
     5581 gtcgctgaac tccatgtctg tcggcaacac ggcctgcgga gtgatcatct cgccggcgcc
     5641 ctgaatcctc atcttcacaa cgggagcttg atccggatcg acccgcacga caaatcgggt
     5701 cagtggcacg cgcgggctga cgttcgtcag accgctcgga ctggtcagct caaagtggta
     5761 agcggcggag gtcgtcggac ggtcggatgc ccggaactcc cgttcgccca cgcattccgc
     5821 aggaccgacg gtgctctgct tgccgccgac gtcacggacc agaatcgccg aggcaatcgg
     5881 ttggctcgcc tcgatggtca agcggatctc gcttccgacc agcgcctcgg caacattctg
     5941 cccttctcgc agctcatagg gttcaagccg cgtataggcc ggtggcgcaa tcccaattcg
     6001 cgcgcgcgtg atactgggcc ggtcaatcac ctcgatcgta aagggatcgg tctgcgcgtc
     6061 tcccccgaca atccggcagg tcaccgtctc ggtcaggtgc tcgaacatgt gtatgaatcg
     6121 taccggctgc ccgcgaacct ccggcatctg agcctggcct cgcaggctgc ccgcgccgtg
     6181 gtagtgaatg taagcctgtc gcggagcttc gtaacccggt actacggcgg ctgaaatggt
     6241 cgcgtcatca ccgcgcggca cgaccagctt accctccgtc aacccttcca caaccaagcg
     6301 gtttttcaga ggccattcaa cgcttctcag cagcacgttt cgctcgaacc aaaggcccat
     6361 cgtaccagga gccgccagca gtaatcccgc tatgccgaca agacccgtca ggcccaacgt
     6421 cgcctgtcga accgctcgtc gatgattgag tgagtcgctg aaccgcaatt gcgacgccgc
     6481 gccgtttgcc tgttcgatga ccttgtcaat gagggcggcc gatccacggt tgaccccctg
     6541 cggatgggcc acaaactcgg tcgccgcaat cagccgaccc cccaattccg ggtgttttcg
     6601 ctcgataatc gttgcgaggt cggcctgggc aaggatcacc gccgccggcc ggatcaggta
     6661 tcgccggctc agccagcaaa gaacacccca gaccgagatc gcctgaaccc atcgcatgct
     6721 cgtgtcgagt cggaaagtat gatcgaccgc caaggtgatc agcaccgccg ccagcaccgc
     6781 cgggctcacg accgccagac cgtccgccag gagatacaga cgaatacgcc tcgcgagcgc
     6841 atccaaccgc acaagcgtat gttgccgaaa ttgcagtcgc gcggattcgc tgacgtttac
     6901 caacatctcg ccgtcgcctc tacagcagat tgaaggcctt acgcaaggcc cactccgtac
     6961 tcaacagtgc caccagcaca aacaaggtcc accagcggtc ccacagcggc gttggccgac
     7021 ctgtggtgac catgctgacc gacttggccg ggatcagcga tggcagtcgg tcgatctcgt
     7081 caagatagag cgatcgcccg ccggccgagc ccgccgccag attctcgagc gattcccggt
     7141 ccagcgccgt ccggcgaaac tccagatcag accgcccgac gcgaagctcg ccccgaatcg
     7201 tcgtcgtgcc ctcgccgcct tcagcgggca ggtcgatccg cagctcatag attccggttt
     7261 ccgtgggggt caactccccg tgataccagc cgggtcgatc gggctgagcc ttcaggacca
     7321 ccacatggcc gggcaccccc tccgcctcaa cggtcatttc aaccgtgtcc cgccccagcg
     7381 gcgagaactt cgagtccaaa agcctcgcct cgatgccgac cgcctcgccc accgggcagt
     7441 ggtccttcgt gggctggatc agcccacgtt tctgaccgcc cagcagctta ccctcgacaa
     7501 gatggcggat cagcgtgatc cagaaccgat tgaagtaccg atctccggcg cgccgccagc
     7561 gccatgtgtc gctccaggcc atgaaagcgg ttcgccccgc cccaacgaac tgagtggcca
     7621 gcagcacgtg ctgacccccg gcgttacgca tctgcgggtt ggagtgacgc agcagaacgg
     7681 tggccaccgg tttttcgcgg ctgaccggat agtgccagta cacgcccggc aactgcgccc
     7741 acgtctgcgc gttttcctcc gagcggtcag cgatattcaa cagcggatgc ccgctgacct
     7801 gcggcggtat cgccggcggc caggccgtct gctggaagta acccagttcg ttgatgatca
     7861 ggtcggcttg gccggcatcg atcaccaccg gcagcagatc gagcagcgcc ttggcggccg
     7921 cctcgcgagc cagttttgaa gtgttcttgc gaccggccac gaacacaaga cccgttcccg
     7981 cctggctgac cagcgtttcg agggtcttgc tccaccccgc gtcgatttcc tccggccgcg
     8041 ggtccatcag aatgatgcag tcatacacgc ccaactcttc atgcttgcga ggcagatgat
     8101 caatgaccgt tccgccgtcg cggacggctt cttcgtcggc cgattgcagc cagcaactca
     8161 cgtccaccgt cgcgtcccgc gtcagcaacc gcgataggta ggtgtattcc caactcggtg
     8221 cccccgccac caggagaacg cgcatcttct tctccaatcc gcgcacgctg gtctcccgct
     8281 cgttatcctc ggcgatgatt tcaccctcga cgggctggat tcgcaccgcc agccgcgtct
     8341 gcagcggacg gataagctga tggtcaaatg tgatcgaact cacgtgatcg tcgggccctg
     8401 cctcgatcgt gcgcgtcgcg acaacgctcg gctgcgatgc atccggcagc cgttgcagca
     8461 gctcgatgat cagcgactgg ccgcaaagcc cttgggcggc gatgcgagcc gtgatcttga
     8521 acgggtcgtt gacgaaaatg ctcgccgggg cctcgatcgc tgcgactgcg gcattgcgcg
     8581 gcggggcggg gtctccgaca cctacggtgt ggatggcgat ccgcttggcg cgggcatatc
     8641 cggcgacggc atccaccgcc tcacccttgt tgaactgccc gtcggacacg accacgaccg
     8701 ccgaaaccgg ccgcccacct tgagattcca ccgcctgccg aacggcagct ccaatatcag
     8761 tcgccggcgc cgacgccggc acggcaatcc cgatcctgcc gcccccagaa ccggcagcgg
     8821 ccgtgtatcg accgaccaga tcgatcccat cgccgaaccg atagcagaga accgggttat
     8881 tccgcaccag ctcacgaatc agccgatgat cgtcgcgggc cagaaccgat ctggcgatct
     8941 cggcccggct caaggtcgca agctcgttca cctgggcgtt gcccagtgcc tgccgcacac
     9001 ggcgttgctc ggtttcatcg gcgtagcggt cgtgaagaga catgctggcc gatccgtcga
     9061 tcagcaagag tgttggcgct tccatctcgc gtcgactgta ggtggcgatc accggttgca
     9121 gccagatcag tgccaagacg gccaatagcg tacagcgtag aagggccagg atcgctcgga
     9181 tcctcgccga agccccaacc cgctgttctc gccgatacag atgcacgctc gcccacagga
     9241 tcagctccag cagaaccatc gcaacaatgg cgtgccaccc ctgcggcagg ccggcgaact
     9301 ccaaccgccg ttcaagcgtc tcggtcgccg tctcagccag cagcctcacg cgcgatgcct
     9361 ccccataagc gccgaccact gacgattggc gccaaaccag caggccagca gttgttccgt
     9421 catcagcgtc cccaccagca ggatcaacaa cgccggccac aattccttgc gggtggcggc
     9481 cgccgagccc tgagtcagct cctctcctcg gatgtattca acaggccatc ctgctgttgc
     9541 ctgctgtagc tcgtcgcgtc gcatccgtcg cagatcgctc tcggtagagt ccacgttcac
     9601 cgcaaccggc tcgaccacat ggccgccgga ttgttccgtg aggtcgatct gatagaagcc
     9661 gggttgatcg gtctgcgtcc agcggattgc catgcctccg gatgacgtgt ccggttcggc
     9721 atgcacggca acagccggtt cttcggggta ggcaggcgat cggaccgcgg ccgttggttg
     9781 ataacgaccg gcatccaagg acagagtcac cggctgcccg acgagctgca tcgccttttg
     9841 ggccggccgg cgagccaggt attgcgtcat ctccatcgca aacacgacga agatcggatg
     9901 gttcgccagg ttgttccatt ccttgtccgc actgctggta accaaccaga cgtgcccggc
     9961 ttggtggtcc ttctgaagaa tcagcgggct cctgtccgcg tcatccagtt gcagcagtga
    10021 aatcgcagtc tgagccggcg tcctcgtcga cgactgcgtc agcggggccg gcgtgatgct
    10081 gactggaaag taagcccaga ccagtacccc ctcaaagcat gctggcatca ggttcgcgaa
    10141 cggccgcaac gcgggatgac tgatgttcat ctcgccgaca cgaaatccgg gactctcgtc
    10201 cggcgcggcc acgggttcgc tcagcttgcc cggcaacagg cccgcgccgt cgcggaacag
    10261 cacctggttg taggcctcaa cgtccacctg atcgcccaga aagaaggcca gaccaccgcc
    10321 cgaggcaaca taggattcca gtcgggccgc cgcgctttcg gtgacccgca ccacgttggc
    10381 cagaatgact gcgtggaagc tcgccagatc ggtctgctca agctcgtctt cgctgatcgc
    10441 tgcaatctcg ttgccgctga actgcggtcc ctgcggacga agagcaaccg acagaaggaa
    10501 ggtctcatcc tgataaggat cggatgatgg ctcgccgttg accagcagca cccggatgga
    10561 tttggccacc ggcaccacac agaagcggcg gttgtcgacc ggcaacgcat ctgcctgcaa
    10621 ctcgaccgtg agcgcagccg agccgacctg cgggaatacc gcctccacgg gcacctcgat
    10681 cgattggccc gccggtatgg ccggtacggt aacggccggc agaccggcct cccccacaaa
    10741 aacctgcatc gtccgggccc gcgatttgct gcgaccgaga ttggccacat gagcgatcag
    10801 ccgcgtgccg gttccggcga cggcctgggc ttggcgactc tcaacaccgg tgaccgcaag
    10861 gttgtcggtg ttttcggagc cgacatcgac caggacgatc ttgagcgagc ggtctgcacg
    10921 atcccagccg gcaagggacg ccaacgggct ttcggccgtg ccgagcttct tgtcgctctg
    10981 gctgccaccg gcgcctggcc cgccattgcc cgccgcttga gcggactcgc ccgcgagcca
    11041 gtcgattcgc tggaagtcgc tcaccagata gatcaccgcc cccgccgaac ggcgggcatc
    11101 aagcaaccgc cggacggccc cgaacgcttc cggaaggtcc gcgcggcgac tcgatggctt
    11161 gcgatcccgc caggtccgcg agaacgcgat cggatcaaga cggccaacgc tcgtctctgt
    11221 gacgatggtc tgctcaggtc gcgacgttgc cacgatggtc agcgcatcgc tcgggcactc
    11281 ctcgcgcagc catccaacca gtcgttccac cgccgtcagg gcccggtcga agacgctcgt
    11341 gtcctgggtg cttgcccccg agcccgcatc cgccaagccc atgctgaacg aatcatcgaa
    11401 aaccacgacg tagtccgtcc ctgcccgcga cccgagcaag ccggcaaagg cctgtggccg
    11461 aacgaaaacg cgagccagca tcatgccggt caatagcatc gccaggcagc gcagcattag
    11521 cagaatcagc tcctcaatgc ggattcgacg gcggttctgg cggttggctt caagcaaaaa
    11581 ctgcgtcgcc gcccatcgaa cgcggcgaaa ccggcgccgg ttgatgagat gaatcacaat
    11641 cgggatggcg gcgcacacca aaccggcgta gaacatcgcc ggagtgatca tcgccgccag
    11701 aacttgattg cccgcaatca tcgatcgtcg attccctacg cctgcttcct gctccgtgtc
    11761 tgttctatgt tcataggacg aaggataaga gatgaaggct gaatagaacc acggtcatcg
    11821 cgagtcttgt tcctttcatc cttccttctt ccgccttcat ccttggtctc tgaaccctat
    11881 ctccccgtct gccgcatcct ccccgccaga aacgccgtca gcgcgccgtc cagcgggtct
    11941 gttgtcgaca gcagccggta gtcgatgcgg ttgttgacgc aggatgcgcg aatgcggctc
    12001 acgaagtctc tgacgacccg cagataggcc gctcgtagcg actgagggtc ggtcagcagt
    12061 tgaattccgg ggtcttccaa tccctcaaac agcgtgttgt gctcaaatgg aaacgtcagc
    12121 tcatcatgat ccagcacctg gagcacgatc agatcttgac ggccgtgacg aagctgctga
    12181 agaccgccga cgatctcatc tgcatcggcc agtagatcgc tgatcagaat gatcaagccc
    12241 cgctggcgaa gctggtcggc aagctgagcc ggaagcatct tcatgtcggt cgtgcggtcc
    12301 ggccgctgcc gctcgatgag ttcgataatc gacgatacct gcgcggggct ggacgccggc
    12361 ggcacctggt cgcggatggc gtggtcaaag agcaccagtc cgcacgagtc ctgctgattc
    12421 aggaggaggt aagccagcga ggccgcaacc gtgcacgcat agtcgaactt cgacatgcgc
    12481 gacccccccc aaccccccct tgccaagggg ggg
//
LOCUS       DAOTPW010000168        12509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4833_length_12509_cov_5.487795, whole genome shotgun sequence.
ACCESSION   DAOTPW010000168 DAOTPW010000000
VERSION     DAOTPW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12509
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4833_length_12509_cov_5.487795"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(491..1606)
                     /gene="ribD"
                     /locus_tag="P5316_14725"
     CDS             complement(491..1606)
                     /gene="ribD"
                     /locus_tag="P5316_14725"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRR86259.1"
                     /translation="MPETFTQQEYVWMRRALALAVRGRGSVEPNPMVGCVLVRGGRII
                     GEGYHRRYGGPHAEVNALKNAGRLARGATAYVTLEPCCHYGKTPPCTDALIAAGVKRV
                     VLAMRDELPPVSGRSVAILRKSGIRVSVGLCEDQARHLNAPYLKLKGSGRPWVILKWA
                     QSIDGKIATRTGDSKWISGEESRRYAHKLRGRVDAIIVGVATVVADDPALTCRHGKPR
                     RTATRVVIDPRLRTPPDARLIRTADKVPTIIVTDRRQTNSAKARRLARLGAEVLGLRS
                     RRSGLDVDGLLRELGRRRMTNVMVEGGGRTLGTFVDAGLADEAVVFVSRRLIGGRQAT
                     SALAGNGPARVADSPRPIWTKLTHCGEDDVYHLRLTR"
     gene            complement(1634..1885)
                     /locus_tag="P5316_14730"
     CDS             complement(1634..1885)
                     /locus_tag="P5316_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86260.1"
                     /translation="MIERDRIIRTWRQGVVRDFVKMEPRIPLGGGRWKPIWPWVYNVA
                     DVLLVAGVAILLLNFWRERREMRAARAKQEAAASAGKSG"
     assembly_gap    1930..1939
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1940..2465)
                     /locus_tag="P5316_14735"
     CDS             complement(<1940..2465)
                     /locus_tag="P5316_14735"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRR86261.1"
                     /translation="MAERTSHCIPSGSAESSEQSFVSAIRSPASHVRLWLVAIIGLVV
                     DLWTKHWAFSNVDPDTGIVIVRNLARFQRSLNTGALFGLGKGWTPVFVVASFLALAFV
                     LYLFVQSGRERWSLHIALGLVLAGALGNLHDRIFVIVDVVQYADRQGRKREPEAGLIN
                     EAKSDDKYLVLENYP"
     gene            complement(2710..3609)
                     /locus_tag="P5316_14740"
     CDS             complement(2710..3609)
                     /locus_tag="P5316_14740"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRR86262.1"
                     /translation="MGKKTTLAKGKKAKTAARSSKGAPKAKAAPSRKRSAKDKPDEKT
                     LRNKSAEKGIKAPVKTPLGASGDKTKAPKASAKAQKADKLVKSSSAETKTAGKTASRD
                     KSTGQPTTPAKTSPGPSKPIPEPREIAGLPKLPAFGSIFERPSRKDAKKPKTSEDGGN
                     GEPSDDSSSETLARTYLSDAELEEFRQLLLSKRQEIVRDVTNLEDEAIRQASGASTSS
                     SMPIHMADLGTDTWEQELTLGLIENERNLLREIDEAIERIDNRTYGICLATNKRISKA
                     RLQAKPWAKYCIEYARRRELGLA"
     gene            4086..4559
                     /locus_tag="P5316_14745"
     CDS             4086..4559
                     /locus_tag="P5316_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRR86263.1"
                     /translation="MISQTAEYALRAVLMLAARDDVPAGTAAIAESTRVPAGYLSKVL
                     QGLARHGLVVSSSGRRGGFRLARKASEISVLQVIQAVAPLARLGQCPLGIESHNGRLC
                     PLHRQLNQATEAFEKALAAVTIAQLLQEPNGRADVAISEERTSPPSVGKDKTGKP"
     gene            4556..5800
                     /locus_tag="P5316_14750"
     CDS             4556..5800
                     /locus_tag="P5316_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HRR86264.1"
                     /translation="MSELIDNRAKRIQTLKEIVQKLHHGAKPEEVRAQLTEIVRHASP
                     GEIAAMEQQLMAEGVTVNEIKSMCDLHAAVVQDILTKQAEGDVLPGHPVDTFRIENAA
                     IRRTLDELNKAVSDLAALPPDDDPGQPLRRARQAANDLMDVDKHYQRKENLLFSVLER
                     HGITGPSKVMWGKDDEARAALKTLQATLRQSYANAGHAAAAIKPAATTAAAAIGSMID
                     KEETILLPMSLQTLTEDEWGQIWQQSSEYGWCLVEPRTGYAPPESVQAQEVAELPHGR
                     SVAFPAGSLTFEQLVGVFSTLPVDLTFVDAEDRVRFFSESSSRIFARSKAIIGRKVQH
                     CHPPDSVGVVERILDDFRSGRQSIAEFWINLHGRFVHIRYFAVRDPKGEYLGTLEVTQ
                     DLTPLRALEGERRLLQYDRPGT"
     gene            complement(5882..6139)
                     /locus_tag="P5316_14755"
     CDS             complement(5882..6139)
                     /locus_tag="P5316_14755"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRR86265.1"
                     /translation="MTTNDENAKPTTITGDHKVGHVVDWYPATIDVLKNHGFTQITNP
                     VMRRTVARHVSIAQAAEMKGVNLEPLLADLNAVIGKGTSGR"
     gene            complement(6308..7663)
                     /locus_tag="P5316_14760"
     CDS             complement(6308..7663)
                     /locus_tag="P5316_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR86266.1"
                     /translation="MQHFGSAGRTLVVLTFLGTCGAAPTARTGEAGEAPVAVRTRANA
                     WVRMAVPEPGESPPVIVDVIDPWGHIVWTGATRTDSIEFLARDWADGVYTVRFTPGGE
                     KKLKVDSEYYGRVWARAGLMVRQLEKKRDPDGILPKELAGASNLLQRIITEYVWQQPD
                     DHVEGHLTFCQQRLGITTATATVRILGSGAADWDGYEGSYRPGRRERSSVFMPPNTMV
                     DFGTDAMRRLRRWGYSAENVEHLFISHEHADHFDPASIAELAANRRDAGLSPLVVHGS
                     KIVCDRLRDHLSKSGRSDMAVLDEMQAGGQTRAGELQVKAVRAAHATSDDPLCFILKR
                     RGATVYYGTDSGYPKAEAFAALAAERFDVFAIDTTFASSDDGREHGDVGDLVRLVDRL
                     RAAGAIDTWTTVLAIHQSRREGPTTIPDMPVFTQRAGFACSYDGMPIPIAFCVTSQPA
                     K"
     gene            complement(7725..8747)
                     /locus_tag="P5316_14765"
     CDS             complement(7725..8747)
                     /locus_tag="P5316_14765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR86267.1"
                     /translation="MHRLGSTTTAAVLLVLGGMAATAAAGAPAAQTKPTSTASSDAGT
                     ENKPAPANKHTYTYKKVDDCEIRADVYRPSADSARTPVIIWIHGGALIMGSRASINRL
                     QLDLYLRAGYTVVAIDYRLAPETKLPGILDDLRDAFAWVREEGPTLFHIDPERVAVIG
                     HSAGGYLTQMSGWCITPRSKALVSFCGYGDIIGDWYTKPSPTYSKQPAVTREQAYSCV
                     GTTAVSEPKSDRVPFYLYTRQQGIWPNVVTGLDPKQQPAAFAAFCPLRNVSADYPPIL
                     LLHGSKDSDVPYEQSVLMDTELSRFKVAHELVTIEGEHGFDSDMRNPRIQKVFDRVLA
                     FLKQHL"
     gene            complement(8850..9260)
                     /locus_tag="P5316_14770"
     CDS             complement(8850..9260)
                     /locus_tag="P5316_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRR86268.1"
                     /translation="MKVFWDTNLFIYLIERHPASHPKVDSLYREHRKSGDEIIASART
                     LGELLAQPLRQGRTDLVRRYTELPTSPAMIRLVAFDRAAAEQYAGKRAQGSIRQPDAI
                     QIACALANRADAFITNDSRLWGVSLPQAMSVRGL"
     gene            complement(9257..9472)
                     /locus_tag="P5316_14775"
     CDS             complement(9257..9472)
                     /locus_tag="P5316_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86269.1"
                     /translation="MVRLTAHYNGKVIVPDEAVQLPEGVPLEVTVRPQGQPGNGDPIL
                     ELSGLGMEVWDGIDPVEYQRREREGWE"
     gene            complement(9639..11015)
                     /locus_tag="P5316_14780"
     CDS             complement(9639..11015)
                     /locus_tag="P5316_14780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRR86270.1"
                     /translation="MTQHGGASRKRSKGGRGPYIQKSGGRPHGDGGDKRAGNTPPPPE
                     HQYPYPWVRLRSASAHPFIYQRMIGQVDPTARPGDVVAVYDKQDHLFGHGFYHDRSQI
                     GLRMLSYEPTAVDEDFFRKRIEQAIAWRRQLLGDDPATDAYRLVHAEGDGLSGLIAER
                     YGDWIAIEVFSLGIFKRLDLIKRVLSESLISSEPAFAQASLCQGLPRRSSKSEGGNPK
                     FVIRADEHVERLEGFEIPTLASDAVPPTITIRENGLRFRVDLRAGHKTGFFCDQRDNR
                     KRLAALCRGAGVLDVCCYTGGFGVYAKKLGQAEAVTGVDLDEDAIELARKNANLNEVR
                     IQHVHADAFGYLRQMQTNGNAYDVVVLDPPKFVGNRDEFQEGSRKYVDLNTLGMSVVR
                     PGGLLLTCSCSGLVSREDFLGMVKAAASRLRRSLQFVDQTGAAPDHPVMANCPESAYL
                     KAVWARVM"
     gene            complement(11280..11663)
                     /locus_tag="P5316_14785"
     CDS             complement(11280..11663)
                     /locus_tag="P5316_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86271.1"
                     /translation="MSLKYLLGALRFVRWFFLIAGSAALAMVLIEIGLEIPRLGFSLL
                     HPLLPALLLVALFACYRLTEVLLLLSLNRVLCELYKRGGKMPDHDLSQRLRGEIARRE
                     RFHKILCGLRGVELSEILDRLYNGY"
     gene            complement(11750..>12509)
                     /locus_tag="P5316_14790"
     CDS             complement(11750..>12509)
                     /locus_tag="P5316_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRR86272.1"
                     /translation="THLGYWGYRYYSPRLGRWLSRDPIGEIAALNLYLAGDNALTNRY
                     DPDGRACVGIGVVGSFTIQIPLILSGSFLGGGGQLSGSRSVYACTGAPHCRVCDAVTL
                     TGLIGGGINISAGVGILVHVGGDPTNGLSFSVGGSVAGPGPAAGLTGEIAIDVDVTNG
                     IVTLTIPRVSAGFSMAVAVRVSGTCTGCSSIFAFNGSAIRKAAIIACITRVVNGVSRA
                     LNSLSQTVVPAAYLPMSFAHNEGRSFSDWSSPPE"
BASE COUNT         2365 a         3807 c         3846 g         2481 t
ORIGIN      
        1 tctgatcgag tacactctca gacgcactgg ggccgacgag ggcgtcggcc ctactgttcg
       61 gccgagaagg ggttctcggt cgagcggagc tcaagatggg ccgacgaggg cgtcggcccc
      121 acttgttctt catcccgtcc atgtcaatct gtgtgatcta cgggccaccc tgcgtgtgaa
      181 ggattccgtt cgccacccga gcttcgcctt gaggcgacgc cgggcaagcc gctggcgcaa
      241 tgggggaccc cgcaatctat catcgacaac gcgcccatcc ggcgtagctg tgctttgccg
      301 aatacatggg ccaccggccg gtcagtggcc agaagcccga caagggtgcc tgcctcactt
      361 ggacatcgct ttacttggac aacgctttac ggcgaatccg gaccatcaaa cgcgcagcgc
      421 aatcgcccgt tcgatccggc cgccgtactt gcgggttggc caaagctgcg atgccggaac
      481 ctgttcaaga tcagcgcgtc aaacgcaggt gatagacgtc gtcttccccg cagtgagtaa
      541 gcttcgtcca gatgggccgg ggcgagtcgg caacacgggc gggaccattg ccggccagtg
      601 ccgaggtggc ttggcggccg ccgatcagac ggcgcgagac gaaaaccact gcctcatcag
      661 ccaagccggc gtcaacgaaa gtgcccaacg tccgcccgcc gccctcgacc atcacgtttg
      721 tcatccggcg gcgtcccagc tctctcagca gaccgtccac atccaggccc gatcgccggg
      781 agcgcagacc gagaacctcc gcacccagtc tggccagacg acgtgccttc gccgagttgg
      841 tctgacggcg gtcggtgacg atgatggtgg ggaccttgtc ggccgttcga atcagtcggg
      901 cgtccggcgg cgtacgcaac ctcggatcga tcaccacgcg ggttgcggtt cgtcggggct
      961 tgccgtgccg acaggtcaga gcggggtcgt cggcgacgac ggttgcgacc ccgacgatga
     1021 tcgcatccac ccggccacga agcttgtggg catatcgccg ggattcctca ccgctgatcc
     1081 acttggagtc tcctgttcgt gtggcgatct tgccgtcgat gctctgggcc cacttgagaa
     1141 tgacccacgg ccggccgctc cccttgagtt tgaggtaagg ggcattgagg tgtcgggcct
     1201 gatcctcgca caacccgacg ctgacccgga tgccggactt ccgaaggatc gcaacgcttc
     1261 ggccgctgac gggaggcaac tcgtctcgca ttgccagaac cacccgcttg acaccagccg
     1321 cgatcagggc atccgtgcaa ggcggcgtct ttccgtaatg gcagcatggt tcgagggtca
     1381 cgtaggcggt tgcgcctcgc gcgagccgcc cggcattctt gagggcgttc acctcggcat
     1441 gggggccgcc atatcgccgg tggtagcctt cgcctatgat ccggccaccg cggaccagaa
     1501 cgcagcccac catcgggttg ggctcgacgc ttccgcgtcc tcggacagcg agagccaatg
     1561 cccgccgcat ccagacgtac tcttgttggg tgaaggtttc aggcatggcg aacggacctt
     1621 tacctgcccg tgatcagccg gacttgcctg cggatgccgc ggcctcttgt tttgcccgtg
     1681 ccgcccgcat ctcccgcctt tctctccaga agttgagcaa cagaattgcc accccagcaa
     1741 ccaggagcac atcggcgacg ttgtacaccc agggccagat aggcttccat cgaccaccgc
     1801 ccagcggaat tcgcggctcc atcttgacga agtcgcggac cacgccttgc cgccaagtgc
     1861 gaatgatccg gtctctttcg atcaaaaaac ggctgccaaa tttcagattt catttgcctt
     1921 aattataccn nnnnnnnnnc cgggtagttt tcgagcacca gatacttgtc atcgctcttt
     1981 gcctcattga tcagcccggc ctcaggttct cgttttcttc cctgacgatc agcgtattgc
     2041 acgacatcga cgatcacgaa gatacgatca tgcaggttgc cgagcgctcc ggccagaacc
     2101 agccccaagg cgatgtggag gctccatcgt tccctcccgc tctgaacaaa caggtagagc
     2161 acaaaagcca gcgcgagaaa cgatgcgacg acaaacaccg gcgtccaccc tttgcccaaa
     2221 ccgaacaacg caccagtgtt caacgaccgc tgaaacctgg caaggttcct gacgatgacg
     2281 atgcccgtgt ctgggtcaac attgctgaag gcccaatgct tggtccacag gtcaaccacg
     2341 aggccgataa tggcgaccag ccacagccgg acgtgcgatg ccggagaacg aatcgccgaa
     2401 acgaaacttt gttcggagct ttctgccgaa ccacttggaa tgcaatgact tgtgcgctcc
     2461 gccatgagtt cccgaagtct gtgaccgcct cctgtttccg aagggggaca accgcgttgg
     2521 tcagaaaacg ccgacactca tcgcctaccg gccggtcgcc gtggggctgg aagcctttct
     2581 gaccaagttg gaccgcctct tggcgggtcg caaccgaacg aaaccgtcac ggagaaacat
     2641 atacgcaaaa cacgcagatt cctcatcgtg aggtaccaag gagcgaggat tgtagccaca
     2701 ttggccgaat caagccagcc cgagttcccg tctgcgggcg tattcgatgc agtacttagc
     2761 ccagggcttg gcttggaggc gagcctttga gatgcgcttg ttggtcgcca agcagatgcc
     2821 gtaagtacgg ttgtcgattc tctcgattgc ctcgtcgatt tctcggagca ggtttctttc
     2881 gttttcaatc aagccaaggg tgagttcctg ctcccatgtg tccgtcccaa ggtcggccat
     2941 gtgaatcggc atgcttgagc tggtgctggc accggaagct tggcgaatgg cctcatcttc
     3001 cagattcgtt acgtctcgga cgatttcctg cctcttgctg agcagcagtt ggcgaaactc
     3061 ctccaattcg gcatcagaca gataggtcct ggcgagggtc tcagacgatg aatcgtcaga
     3121 tggttcgccg ttcccaccat cttcgctggt tttaggcttc ttggcgtcct tgcggctcgg
     3181 gcgctcgaaa atgctcccaa acgcagggag tttcggaagg ccagcaatct cccgcggctc
     3241 cgggatcggc tttgacggtc ccggactcgt tttggctgga gtggttggct ggcctgtcga
     3301 cttgtctcgc gaggcggtct tacccgcagt cttggtctcg gcagagctcg atttgaccag
     3361 cttatcggcc ttctgtgcct tcgccgatgc cttgggcgcc ttggtcttgt ctcccgacgc
     3421 acccagcggg gtcttgaccg gggccttgat ccctttttcg gccgacttgt tcctcagagt
     3481 cttttcgtca ggcttgtcct tggcggatcg cttccggctc ggcgcagctt tcgccttggg
     3541 ggcccccttg gaagatcggg ccgccgtttt ggccttctta cccttggcta atgtcgtttt
     3601 ctttcccact gtgtacctgc accgaacggg tcgctaaatt aacgtaacct cttgcaacac
     3661 aacaagaaaa gttaaggcta tcgtcttgaa agtataggtg cgaaaacccg tactgtcaag
     3721 ccgcagtccc gatgatcgaa agcaaggcgc ttatcggttg gccctccgcg cttctaaaac
     3781 agcaacaatc atgatgggcg atcttcaaac aacactgggc ctgcacgcag agccgagctg
     3841 gctcctgaaa agccaacccg acactcagtc gcgagacagt tgggcggcta ccaagatgca
     3901 taggacgtca tcgggttcac agggggagca ggacggccga cttgcctgat cgtcccaata
     3961 aaacctggac ctccatgttg aaattcgtgc gctggctgcc taggcttgga tgcggcgctg
     4021 gttccacgcg cagacccaga tcggccgttt cagggtttca gaatatcggg cagggtgggc
     4081 cagtcatgat ttcgcagacc gccgagtacg cgctacgcgc cgtgcttatg ctcgcagcac
     4141 gagatgatgt tccagcgggt acggccgcaa ttgcggaaag caccagagtt cctgccggat
     4201 acctgtccaa ggtcctccag ggcctggcgc gtcatgggtt ggtggtttca agctcgggcc
     4261 gtcgcggcgg tttccggctg gcccgcaagg ccagtgagat ctcggtgctt caagtcattc
     4321 aggcggtggc tccgcttgcc cggcttgggc aatgcccgct tggaatcgaa tctcataacg
     4381 gtcggctttg tccacttcac cgccaactta accaggcgac ggaggcattc gagaaggctc
     4441 ttgcggcggt caccattgcg caattgctgc aggaaccaaa cggtcgcgct gacgttgcca
     4501 tctcagaaga acggacaagt cccccttccg tcggcaaaga caagacgggt aaaccatgag
     4561 tgaactgatc gacaatcgtg ccaagcgaat ccagacactg aaggagattg tccagaagct
     4621 tcatcatggg gccaagcccg aggaggtgag ggcccagctc acggagatcg tgcgtcacgc
     4681 cagcccgggg gagatcgctg ccatggagca gcagcttatg gccgagggcg tgacggtcaa
     4741 cgaaatcaag agcatgtgcg accttcacgc cgcggtggtg caggatatcc tcaccaagca
     4801 agccgagggc gatgtactac ccgggcaccc ggtggacacg tttcgtatcg agaacgcggc
     4861 catccggcgc acgctggatg agctgaacaa ggctgtcagc gacctggccg cgctgccgcc
     4921 ggacgacgat ccgggacagc cgctgcgtcg ggcgaggcag gcggccaacg acctgatgga
     4981 cgtcgacaag cactaccagc ggaaagaaaa tttgcttttc tcggttcttg agcggcacgg
     5041 gatcaccggg ccgtcgaaag tgatgtgggg aaaagatgat gaggcccggg cagcgctcaa
     5101 gacgctgcag gcgacgctgc gacagagcta cgcaaacgcc ggccacgcgg ctgcggcgat
     5161 caagcccgcg gcgacgacgg cagcggcagc gatcggttct atgatcgaca aggaggaaac
     5221 cattcttctg ccgatgtcgc tccagacgct gacggaggat gaatggggac agatctggca
     5281 gcagtcgtcc gagtatggct ggtgtctcgt cgagccgcgg accggttatg ctccgccgga
     5341 gtccgtgcag gctcaggagg tggcggaact gcctcacggc cgatcggtgg cttttccggc
     5401 cggcagcctg accttcgagc agcttgtcgg cgtgttctcg acgctgccgg tggatctgac
     5461 cttcgtggac gccgaagacc gggttcggtt cttctccgag agcagcagcc gcattttcgc
     5521 tcgcagcaag gcaatcatcg ggcgcaaggt ccagcactgc cacccgccgg acagcgtggg
     5581 cgtggtggag cggattctcg acgatttccg atcggggcgg cagagcatag ccgagttctg
     5641 gatcaacctg cacggtcgat tcgtgcacat ccgctacttc gcggtgcgcg atcccaaggg
     5701 tgagtatctg ggcactctcg aagtgactca ggatctgacg ccgctacgag ccttggaggg
     5761 cgagcggcgg ttgctgcagt acgataggcc tgggacgtaa gtcactggct cgggtccggc
     5821 gggttcttgt gagcgcggac gggttggttg cggtgggcga gactctcccg caacgagagc
     5881 tttaccggcc gctggtaccc ttcccaatca ctgcgttgag atctgccaga agcggttcca
     5941 gattgacgcc cttcatctcg gctgcctggg cgatcgagac atggcgtgcg acggtgcggc
     6001 gcatgaccgg gttggtgatc tgggtgaagc cgtggttttt gagcacgtcg atcgtggccg
     6061 ggtaccagtc cacgacatgg cctaccttgt gatcgccggt gatcgtcgtt ggtttcgcgt
     6121 tttcatcgtt tgtcgtcatg cggagctccg tgcgaagatg cgctcgttca agcgacaaga
     6181 atcgacaacc caggctcagg aagagcctga tccatctcat ccggtggacg gacagactat
     6241 cagacgaacg gatcttcgga caagaagacc gacaagccgg cggccggtga gacgggcttt
     6301 ctgcagccta cttggccggt tggctcgtca cgcagaacgc gatgggaatt ggcatgccgt
     6361 cgtaactgca ggcaaagccg gctctttgcg tgaagacggg catgtcgggg atcgtcgtcg
     6421 gcccctcgcg gcgcgactga tgaatggcca ggacggtggt ccaggtatcg attgcgccgg
     6481 cggcccggag tctgtcgacg agccgcacaa ggtcgcccac atcaccgtgc tcgcggccgt
     6541 cgtcactcga tgcgaaggtc gtgtcgatag cgaacacgtc gaatcgctcg gctgccaggg
     6601 ccgcgaaggc ctcggctttc ggatagccac tgtcggtacc gtagtaaaca gtggcgcctc
     6661 gccgtttcaa gatgaagcac agcgggtcat cgctggttgc atgcgctgct cgcaccgctt
     6721 tcacctgtag ctcgccggct cgcgtctgac ctcccgcctg catttcgtcg aggacggcca
     6781 tgtcactcct gccgctcttg gacaggtgat ctctgaggcg gtcgcagacg atcttgctgc
     6841 catgcacaac cagcgggctg agcccggcat cccttcggtt ggcggccagc tcggcgatac
     6901 tcgcggggtc gaaatgatcg gcgtgctcgt ggctgatgaa gaggtgctcg acgttctcgg
     6961 ccgagtagcc ccagcggcgc agcctgcgca tcgcgtcggt gccgaagtcg accatggtat
     7021 ttggcggcat gaacactgac gatcgctcgc gccggccggg gcggtaggag ccctcgtagc
     7081 cgtcccagtc ggcggccccc gagccgagga tgcggacggt ggccgtagcc gtcgtgatgc
     7141 ccagccgctg ctggcagaag gtcaggtggc cctcgacgtg gtcgtcgggt tgttgccaca
     7201 cgtactcggt gatgatccgc tgcagcaggt tggacgcccc ggccagttcc ttgggcagta
     7261 tgccgtccgg gtcgcgcttc ttctcgagct gtctcaccat cagcccggcc ctggcccaca
     7321 cccggccgta gtactcgctg tcgaccttca gcttcttctc gccgcccggc gtgaatcgca
     7381 cggtgtagac gccgtcggcc cagtcgcggg ccagaaactc gatcgagtcg gttcgggtcg
     7441 cgcccgtcca gacgatgtgg ccccacggat cgatcacgtc gacgatcacc ggcgggctct
     7501 cgcccggctc gggtacggcc atccgcaccc aggcatttgc ccgggtgcgc acggccaccg
     7561 gcgcttcgcc cgcctcgccc gtccgcgccg tcggggcggc gccgcaagtg ccgaggaagg
     7621 tcaggacaac gagtgttctt cctgccgatc caaagtgttg catgattcgc ttgtcatgcc
     7681 aacggctcgg cgtgggcatg cctgtatgtg attgccgaca gttctcacag atgctgcttt
     7741 aggaaagcca gcacgcggtc gaacactttc tggattctcg ggttccgcat gtctgagtca
     7801 aacccgtgct ccccctcgat ggtgaccagc tcgtgcgcca ccttgaagcg ggacagctcc
     7861 gtatccatca acacggattg ctcgtagggt acgtccgaat ccttgctccc gtgcaggaga
     7921 aggatgggcg ggtaatccgc ggagacgttc cggagcgggc agaacgcggc aaacgcggcc
     7981 ggctgctgct tcgggtcaag ccccgtgacc acgttcggcc agattccctg ctgccgggtg
     8041 tagagataga agggaactcg atccgatttc ggctcggaga ctgccgtcgt cccgacgcat
     8101 gagtacgcct gctctcgtgt gacggcgggc tgcttcgagt aggtcgggct cggcttggta
     8161 taccagtcgc cgatgatgtc tccgtatcca cagaaggaga ccaatgcttt ggaccgaggg
     8221 gtgatgcacc acccgctcat ctgagtcagg tagcctccgg ccgagtgacc gatcacggcc
     8281 actcgctcgg gatcgatgtg gaacagagtc gggccttcct cgcggaccca ggcgaaggcg
     8341 tcacggaggt cgtccagtat gccgggcagt ttggtctccg gagcaagtcg gtaatcgatg
     8401 gccacaacgg tgtagccggc gcgcagatac aggtccaatt gcaggcggtt gatgctcgct
     8461 cgcgaaccca tgatcaacgc tccgccgtga atccagatga tcacgggtgt ccgagccgaa
     8521 tccgccgacg ggcgatagac gtcggcacga atctcgcagt cgtcgacttt cttgtatgtg
     8581 taggtgtgct tattggcagg cgcaggcttg ttctcggtgc cggcatcgga ggacgcggta
     8641 cttgtcggct ttgtttgagc agcgggcgcc cccgctgccg ccgtggcagc catgccgccg
     8701 agaacaagga gaacggccgc cgttgtagtc gaaccgagcc tgtgcatttc ttcacacccc
     8761 ctaccgctga ggcgatggcc atccgactat ctcagtcata gcgcaaacaa ctcaaccaat
     8821 ggcgtctgat cttcgccgca gactgcgagt caaagacccc ttaccgacat cgcctggggc
     8881 agtgaaacgc cccacagtct cgagtcgttt gtgatgaaag cgtctgctcg gttcgccagc
     8941 gcgcacgcaa tctgaatggc gtctggctga cgaatggaac cttgtgcgcg tttgcccgca
     9001 tattgctcgg ccgcagcccg gtcaaaggca acaagcctta tcattgcggg cgaggtcgga
     9061 agctcggtgt atcttctcac gagatcggtt cttccttgcc gaagcggttg ggcgagcagt
     9121 tcgccaagtg tgcgcgctga tgcaatgatc tcatctccgg atttgcgatg ctcgcgatac
     9181 agagagtcaa ccttggggtg ggaggcgggg tgtcgttcga tgagatagat gaacaggttg
     9241 gtgtcccaga acaccttcat tcccagccct cgcgttcgcg acgctggtac tcgacagggt
     9301 caatcccgtc ccagacttcc atgcccagcc cgctcagttc gaggatggga tcgccattgc
     9361 cgggttggcc ttggggacgg acggtgacct ccagcggaac cccttcagga agctgaacgg
     9421 cttcatccgg aacaatcact ttgccattgt agtgggcagt caggcgtacc atcgagcacc
     9481 tcaggacgca gtacagtata gctgacccac ggatcgactg ccagccctcg tcccgctgag
     9541 agttgtccgc caatgaagct ctggtctccc gtgcacgggc aaagacggcg ttcgtaggac
     9601 cggttgcctc aattcaccat tttgcgttcg acattccctc acatcactcg cgcccacacc
     9661 gccttgaggt acgcgctctc cgggcagttg gccatgaccg ggtgatccgg ggcggcgccg
     9721 gtctggtcga cgaactgcag ggaccgccgc aatcggcttg cggctgcctt gaccatgccg
     9781 aggaaatcct cgcgcgagac caggcccgag catgagcagg tcagcagcag gccgccgggg
     9841 cgaacgacgc tcattccgag cgtgttcagg tcgacgtatt tgcgggaacc ctcctggaat
     9901 tcgtcgcggt tgcccacgaa tttgggcgga tcgagcacga cgacgtcata agcgttgccg
     9961 ttggtctgca tctgccgcag gtagccgaag gcgtcggcgt gaacgtgctg gatgcggacc
    10021 tcgttgaggt tggcgttctt gcgagccaat tcgatggcgt cttcgtcgag atcgacgccg
    10081 gtcaccgcct cggcctgacc gagcttcttg gcgtaaacgc cgaagccgcc ggtgtagcaa
    10141 cagacgtcga gaacgccggc tccgcggcac agtgcagcga gccgtttgcg gttatcgcgc
    10201 tggtcgcaga agaagccggt cttgtgtccg gcgcgaaggt ccacgcggaa tcgcaggccg
    10261 ttctcgcgga tggtgatggt cggcgggact gcatcggatg ccagggtggg gatctcgaat
    10321 ccttcaagcc gttcgacgtg ctcgtcggcg cggatgacga atttagggtt cccgccttcg
    10381 ctcttcgagc tacgccgtgg caagccctgg cacagcgaag cctgggcgaa ggccggttcg
    10441 cttgagatca gggattcgct gagtactcgc ttgatcaggt caagccgctt gaagatgccg
    10501 aggctgaaga cttcgatggc gatccagtcg ccgtatcgtt cggcgatcag gccgctgagc
    10561 ccgtcgccct cggcgtggac gagtcgatag gcgtccgtgg cggggtcgtc gccgagcagt
    10621 tgcctgcgcc acgcgatcgc ctgctcgatc cgcttgcgga agaagtcctc atctacggct
    10681 gtcggctcgt acgacagcat ccgcaacccg atctgtgagc ggtcgtggta gaagccgtgg
    10741 ccgaagaggt ggtcctgctt gtcataaacg gccacgacgt cgcccggcct ggccgtcggg
    10801 tccacctgtc cgatcatccg ctggtagatg aacgggtgtg cgctggccga tcgcagccga
    10861 acccaggggt aaggatactg gtgctcgggc ggcgggggtg tgttgccggc ccgcttgtcg
    10921 ccgccgtcac cgtgcggccg gccgccggat ttctggatgt acggtccccg gccgcccttg
    10981 ctgcgctttc gagatgctcc accgtgctga gtcatgagag catcatccgg gacaagacgc
    11041 ggaggtgcaa gaaggagggg cggggggaat gtggaattga gaatggagaa tgggccgtgt
    11101 tctgccgtcg cccatgaacc tcaccactgg tcaactccgg aaacagcggt cattcggcac
    11161 aggacgtcca atccacaagg gctttcctaa ggcggaccgc ctttttgcgg gccgcaaccc
    11221 aagcgttctg cgatccggaa acatgcgcgc aaaatgcgca ccagtcgcgg cggaagggac
    11281 taatatccat tgtacaggcg atccaggatc tctgacagtt ctacgccgcg cagaccacaa
    11341 agaatcttgt gaaaccgctc ccggcgagcg atctcccccc taagccgttg cgataagtca
    11401 tgatccggca tctttcctcc acgcttatac aattcgcaaa gtactcggtt gagactcagc
    11461 agcaacaaca cctctgtgag tcggtagcaa gcgaagagcg ccaccagaag caacgccggg
    11521 agtagcggat gaagcagcga gaatccgagg cggggaatct caaggcctat ttcgataagt
    11581 accatcgcca aagcagcaga cccggcaatc agaaagaacc atctgacgaa ccttagtgct
    11641 ccgagaagat acttcagaga catgacgtcc tagtctcctc atcatcggtt ttcaagcatc
    11701 ggctgcggaa tagaaaactt cgccggcacg ctgcggactc cggtacatct tactcaggcg
    11761 gagaagacca atcagagaag cttcttcctt cgttatgtgc gaaggacatt gggagatagg
    11821 cggcgggtac aacggtctgg ctcaggctat tcagagctct gctgaccccg tttaccaccc
    11881 tggttataca agcaatgatg gccgccttcc tgatcgcaga accgttgaag gcgaagatgc
    11941 tggagcaacc cgtacacgtg ccgctgactc gcacggccac ggccattgaa aagcccgcgc
    12001 tcactcttgg gatggtcagc gtgacgatcc cattcgtgac gtcaacatcg atcgcgattt
    12061 caccggtgag ccccgctgcc ggtcccgggc cagccaccga cccccctacg gagaaactga
    12121 ggccgttggt tgggtctccg ccaacatgta ccagtattcc aactccagct gaaatgttta
    12181 ttcctccacc aataaggcct gtaagcgtga cagcatcaca taccctgcag tgcggagccc
    12241 cagtgcatgc gtacacgctg cgggatccgc tcagttgtcc tccgcctcca aggaaactgc
    12301 cggatagaat gagcggaatc tggattgtaa aagagccgac cacacctatt ccgacgcagg
    12361 ctcggccgtc agggtcatac ctgttcgtca atgcgttgtc accggccaag tagaggttca
    12421 gtgctgctat ctctccaatc ggatccctgc tgagccatcg tccgagtcgt ggggagtagt
    12481 agcggtagcc ccagtagccc aggtgagtt
//
LOCUS       DAOTPW010000169        12504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4837_length_12504_cov_5.106916, whole genome shotgun sequence.
ACCESSION   DAOTPW010000169 DAOTPW010000000
VERSION     DAOTPW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4837_length_12504_cov_5.106916"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="P5316_14795"
     CDS             complement(<1..1904)
                     /locus_tag="P5316_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR86273.1"
                     /translation="MVCFVEQEVPYVLRIFDGARLAALAFVCLAGACLPRPILADIFV
                     RWQDPQANAWVLAPSAEATIDQANRRITFITPVASRVYWVYAGSGIVPQGEPACAGFS
                     PEDIVEICGDSTTVSFSVQIVAPFQGPNGPVPRFGCDGAANVNAISLSNTNARIAASR
                     ISGNCGSIQLKTGQSGSIGRVDLNIAGSLTRDIDGDGYIDVDRPSSFSSLGTSGLIIG
                     GSIANGISLTITGPTGGSLSVAGDIDGNVTINSFSSTGNICAANISGLSSESQLPPNI
                     DIGPDGGGWKVCGQDLECDTDGVPDGADNCKCANNPDQTDTDGDGVGDVCDTCTDTDG
                     DGKGNPGFPANTCPADNCPDVSNPGQQDTDGDQVGDACDNCPTISNTTQVNSDHDALG
                     DSCDNCPTVYNPDQTDTDGDDLGNACDPDDDGDDIADAIDNCPLISNSTQTDTDGDGV
                     GDACDNCLNVLNPDQRDTDRDGIGDVCENVIYVKPQATGLGDGTSWENATTLQAALTA
                     AQEHDDIWVAAGTYKPSSPSGRAATFQLQNDVSVYGGFAGIAAETPATFNLADRDFTT
                     NQTVLSGDIGTVGTQTDNCYHVVTGSGTNSTAILDGFTIRDGNANGSATSQKRGGGLY
                     LAAGSPAIVN"
     gene            complement(2783..3355)
                     /locus_tag="P5316_14800"
     CDS             complement(2783..3355)
                     /locus_tag="P5316_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86274.1"
                     /translation="MTTSMMRGFLALCAILAVTTVLAPAAGAAQVISVGAEYGFLLPN
                     PPYTPDTLRIQITLGYGEPENTIGEQVWIMGPGVYDLSGDPDMGCFLNMASDGIPEFL
                     SVAIFDAAGASISCPDALGEPQTLGVEPWTVDLNGLDATGIQLSVWDLSLAGGCIVHW
                     EVTGLPEPATLLSAIPLALVLVRVWEPRCW"
     gene            complement(3352..5031)
                     /locus_tag="P5316_14805"
     CDS             complement(3352..5031)
                     /locus_tag="P5316_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR86275.1"
                     /translation="MTKRRAIAIVCLLVSLTLLRTELFGDCAPGGTPPAVPGLGGGDT
                     NTHIFSNQCLARVGTDVTITISAIADLGCNPWDSTHDLGDCDTCSNIPCCDADPEEKY
                     LTVTANNHALDPGVTDSTNMPAEGKFFKEWNLDCTGTSPACAKAGRSPCDGTEPLSAN
                     LHAVTIPAETWNSWLGASGNTMTIRVTSTGAMNSSLAAYCAGLGFASCPYGGSCDGPC
                     GPDTYSSVSISYLAPAKPSIGSLSDSPDPVAQGSNLTLTANNVTDADGYVTAVEFYRD
                     TNGNGTIEPGTDVLLGIDSSLAGGWTWTGSTASFPVETNRYLARAKDDMDQWGNTAST
                     TGSVEIAEACCRTDFSCQDLLPTVCAANGGTPAGTGTSCGTADSDGDGILDTCDNCPD
                     DSNPNQSDHDNDGIGDACENVIYVKPQGSGNGTTWENATTLQTALTAAQTHDDIWVAA
                     SPSPTDYYKPSVPSGRAATFTLKNNVSVYGGFAGTEEPATFRLADRDFTTNQTVLSGD
                     IETNTGCFLASKDFDPPAPGCISRDHGMAGWVSPDESVLRETLLPTSGRTP"
     gene            complement(5028..5639)
                     /locus_tag="P5316_14810"
     CDS             complement(5028..5639)
                     /locus_tag="P5316_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86276.1"
                     /translation="MIHQAIRSLIVLFAVANAATATPILLSSYEAREALDDIRYADVS
                     RVGLMLELYSLAPPYQSFTVGGSVYWQDGQQGTVDFTTEDAAGFNGLVGLVTNGIEDQ
                     FSVLALFEEGGGGSGGPESWLLGRSPDLAGYRVDFVRLSVEQLSIGPSPGHPGASRLD
                     YQAKWEMWGEVVPEPTSSAVFVLGVVSIMSRWRVSGTSRRSSR"
     gene            complement(5687..6304)
                     /locus_tag="P5316_14815"
     CDS             complement(5687..6304)
                     /locus_tag="P5316_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86277.1"
                     /translation="MTIRGLSGTLVFFLVVMKVAKASPILLATLDARVTLGTPQVVES
                     AWPVLFYLMEDNSIRGMLFNEFAIQPQFPTDIDITIFSAGDPDFGEFTALLSNGRNDD
                     KLKGAWNNNAGLLGGGGGQDLFDLELHVPSFGPDRVGYRIESIREVVTVDLRSPGVDP
                     GGDGIWTDWEISAGRYEFYGVPVPEPTSSLLCCASALLVIRARSK"
     gene            complement(6449..7144)
                     /locus_tag="P5316_14820"
     CDS             complement(6449..7144)
                     /locus_tag="P5316_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86278.1"
                     /translation="MKQDVILGANDGADGSRDSAGGEVMIARSLICAMAVASMALNTA
                     NAAPILLASHSSDVAGGGAPESEPQVEFILQLPGSLEDKYGLGVGAFWEAPGQLDFTN
                     AGDPGFAIFALSATNGIAEEFRFWDLFPSGGGHISPPAPESLLFGVSPDLFGYELTQV
                     RLIVENLVFQPWTPDPLNNPDLQGFTYSAEITYEFYGLPVPEPSSLAVLAVPAVIAVI
                     VDNCRCSRRQCST"
     gene            complement(7654..8832)
                     /locus_tag="P5316_14825"
     CDS             complement(7654..8832)
                     /locus_tag="P5316_14825"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR86279.1"
                     /translation="MAAAMVSAVCASISRAEKPYAAPICPHILGWPDDVKKWAEGAPW
                     IKALDITSCAIAKERGARVFYRVWDHASGPDGDPKIGGKKFGQEILDRLKHLPKEKWP
                     DAIGFQNEFNDVGPETARCFIDMYDTLRKGGYEGLIVFGSYGPGGPTDSKEWERPHVK
                     EACLKADAIETHEYWDLTVKHYDTWLAHRHVRAMNDYPWLRSKPWFIGEFGSDGVNKG
                     EDPQKRSGWRDREKLTADEYIKQIEIYRYGDPKNEVVAAAENVMAVFLFQQGAPPHMW
                     QGWETRGTKVMDYMKSTWKPTHAFITGKVTDSSGKPVADVQVAISPSDKKATTDTKGV
                     YWYFAVKPGEYTLKATKPGLKAASVKLSLKPGDILEANLALKSPVRDPAKKNGSTRTE
                     "
     gene            complement(9080..11020)
                     /locus_tag="P5316_14830"
     CDS             complement(9080..11020)
                     /locus_tag="P5316_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR86280.1"
                     /translation="MKSRRAETSIAVACCAAILVLSPPAEARRILEVGHQPYLQLGDA
                     PLATTGPGVGERDQIVIVWQTTESGMGDAPTDFFEVGYRRVGEPGLTPAAPIGTLATG
                     SGTRVNHWVTITGLDYGEQYEYQVIHKREPGNPVVVAAYAGAFSTRKRHDDTAPFSFA
                     VTGDNAHARLPETVPVFEMVMGRIAALDPAFVMLVGDGVQDLGEHPEYDYRFNESLVP
                     NQSAFVRNHVEYFCMGNHEAYTNDGGPALDNYHVPSPVSGVTSPVAGPVGESPEKNYS
                     YDYGLAHFTIFDATAWGGPGNSDARQSAIVTWLQADLNASSQPWKIVVGHYPPKSGVG
                     RPDTEGDMAAEVIPVLVQAGADVLLCGHIHTYQRSFPLTGYAGGDVTYLTDQSPLFVN
                     RDLYIKGDHVIEIVVAPGGMPFDGNVPNRLPGWLARAFGENNGGRAGPMLVEVDQRFL
                     KLRYVAADNGEVMDEVVIHVPAPVITLSHTVFNRTIFLGEDLPKDTFTIRNGGIGTLN
                     YTIEDDAFWLSQSPTTGDSTGEPDPIDISYDEVSGLEVGQYTAVITVSDEEAANNPQT
                     ILVNLTVRTVSPDFDYDGDVDQEDFGHLQACLTGTGATQTEPRCQNADVNNDEVIDGI
                     DISILRGCLSGPGVRADPNCAD"
     gene            complement(11243..11998)
                     /locus_tag="P5316_14835"
     CDS             complement(11243..11998)
                     /locus_tag="P5316_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86281.1"
                     /translation="MRLLTIVQAIVVGAVLAGTVQAVNLGLEGTYLAASAELDQRAGA
                     GTGGGTMQPPYTDDSELNFAGSIAPDWYVAYPSWIGPAAVHWRLSKEPADKTEGECSM
                     KIMIDDEQMNPRDIYVGTKFPTVAGVEYNVFFDMKLGNMNGLTTKPFRAQYAVRADGS
                     LSANDMKAIEDGPGSITFPAANFGDGLWHTYQYTFTATGPEASLIIYTRAREGAQVQG
                     NFHLLDNLVVVPEPTLSALLMAGGLPALLRRRR"
BASE COUNT         2478 a         3945 c         3575 g         2506 t
ORIGIN      
        1 cagttcacaa tcgcggggct gccggccgcc agatacagcc cgcccccgcg cttctggctg
       61 gttgcggaac cattggcatt gccgtccctg atggtgaacc catcgagaat cgcggtgctg
      121 ttcgtgccgc tgccggtgac gacatggtaa cagttgtccg tttgggtccc gactgtcccg
      181 atgtcgccgc tgaggactgt ctggttcgtt gtgaagtcgc gatcggccag attgaacgtg
      241 gccggggtct cggcagcgat accggcgaat ccgccgtaga ccgacacgtc gttctggagt
      301 tggaaggttg ccgctcgccc gctcggtgag ctgggtttgt acgtgccggc cgccacccag
      361 atgtcgtcgt gttcctgggc cgcggtcaga gcggcctgca acgtggtggc attctcccag
      421 cttgttccgt cacccaagcc ggtcgcctga ggcttaacgt agatcacgtt ctcgcagacg
      481 tcgccgatac cgtcccggtc ggtgtcccgt tggtcggggt tgaggacgtt gagacagttg
      541 tcgcaggcgt cgcccacgcc gtcaccgtcg gtatccgttt gagtcgagtt tgagatgagg
      601 gggcagttgt cgatcgcatc ggcgatgtcg tcgccgtcgt catccggatc gcaggcgttg
      661 cccaagtcgt cgccgtccgt gtcggtctgg tcggggttgt acaccgtcgg gcagttgtcg
      721 caggagtcac ccaacgcgtc gtgatcactg ttgacttgcg ttgtgttgga gatcgtcggg
      781 cagttgtcgc aggcatcgcc cacctggtcg ccgtcggtgt cctgctggcc gggattcgag
      841 acatccgggc agttgtccgc gggacaggta ttggctggaa agcccggatt gcctttcccg
      901 tctccatcgg tgtcggtaca ggtgtcacac acatcgccga ccccgtcgcc gtcggtatcg
      961 gtctggtccg gattgttcgc gcacttgcag ttgtccgcac cgtcaggtac gccatcggtg
     1021 tcacattcaa gatcctggcc acataccttc cagccaccgc cgtcgggccc aatatcgata
     1081 ttgggaggaa gctgagattc agagctcagg ccgctgatat tagccgcaca gatattgccc
     1141 gtgcttgaaa agctgttgat cgtaacgtta ccgtcgatat cgccggcgac agacagcgag
     1201 ccgccggtcg ggcccgtgat ggtcaacgag atgccgttgg cgatgctgcc tccgatgatc
     1261 agacctgacg tgccaaggct gctgaaactt gacgggcggt cgacgtcgat gtacccatcg
     1321 ccgtcaatgt ctctggtcag cgagccagcg atattcaagt ccactcgacc gatgctgccg
     1381 gattgcccag tcttgagttg aatgctgccg cagttccccg aaatgcgaga ggcggcgatc
     1441 ctcgcgtttg tgttgctgag actgatagcg ttgacgttcg ccgcaccatc gcaaccgaaa
     1501 cgaggaacgg gcccattggg tccttggaaa ggggcgacga tctggaccga gaagctcacc
     1561 gtcgtggagt ccccgcagat ctcaacgatg tcctcaggcg agaagccagc acacgctggt
     1621 tccccttgcg ggacaatgcc ggagcctgcg tagacccagt acaccctgct cgctaccggt
     1681 gtaatgaacg tgattctcct atttgcctgg tcaatcgtag cctccgccga gggcgccagg
     1741 acccaggcat tggcctgagg atcctgccac cggacaaaaa tgtcagccaa gataggtcta
     1801 ggcaaacacg cgccagccag acacacaaat gcgagagcgg cgagccttgc accgtcgaat
     1861 atgcggagca cgtagggcac ctcctgctct acaaaacaga ccactgagcc cctcgcgact
     1921 gccccgttac ccacgacatc tcaagcatgc tgatactgcc gcccatcgtc cgcagggaca
     1981 acgtctgtgt cggacaagta gggcaatgaa ggctcccaac agcaccgccg taccggtcgt
     2041 aggctcaggg accccaagca cctcccagcg caatccaagt ccgccagcag gagagagatg
     2101 ccaaacgcac agttgaatac cggtggcttc aagcggcgcg atgtctgtga agggaacggg
     2161 cctgccaaac gtctcgggct cagcgagagc atcgacataa ctgatgcgtg cccccgcggc
     2221 atccatgatg tctaccgaga gatcgtccat aagcccgttg ctggcaatag agagaaacag
     2281 cgacatatct gggtctgacg agaggtcaaa aacgccgggg ccggtcagcc agacctgatc
     2341 cccgatcgcg ctttcggaat cttcatatcg aagcacgatt tgaatacaaa gagtatcgac
     2401 attgtagggc gggttaggca gcacaaagga gtagtccgcc ccctgagata ttatactggc
     2461 accatcagca gagcccacac caagtccaca cacaagtgcg gtcacgaagc tactcaccgg
     2521 tcttctgagc attggtcact cccttcaccg aaaatgccct tgacaagcac actgcgacta
     2581 ccgcatccga aaccacaggc gtcctcccta ctgtttccat ccctgtccca atccccgata
     2641 ctgctaacgg gtcgggcgta ctgaacccga gcgggctgcc ggaacagagg atattcgcac
     2701 cttgagccga gctggcaacc aatgtcatcg ccagaatgaa tcgttcgagc attgccatcc
     2761 cgttcttatt cgacgagcca attcaccaac aacgcggttc ccacacacga accaggacaa
     2821 gtgccagggg aatggctgac aacagtgttg caggttcagg gagccccgtt acctcccaat
     2881 gcacaatgca ccctcctgcg agcgaaaggt cccatacact caattgaata cccgtggcat
     2941 cgagcccatt caggtcaact gtccacggtt ccactccaag agtctggggc tcgccgagag
     3001 cgtccgggca gctgatagac gcgcccgcgg catcgaaaat ggcaacagag aggaactccg
     3061 gaattccgtc gctcgccatg ttcagaaaac accccatgtc tggatcgcca gacaggtcat
     3121 agacgcccgg ccccattatc cagacctgct cgccgatggt attctccggt tcaccgtagc
     3181 ccaaggtgat ctgaatacgg agtgtgtctg gcgtatacgg gggattgggc aagaggaacc
     3241 cgtactcggc ccccaccgag atcacctgcg ccgcaccggc cgcaggggcg aggacggtcg
     3301 ttacggcgag gatcgcacaa agagccagaa aacctctcat catcgatgtt gtcatggagt
     3361 cctccctgaa gtcggaagca acgtctctcg aagaacactc tcgtctggac tcacccaccc
     3421 tgccatgcca tggtcgcgac ttatacatcc tggggctggc ggatcaaagt ccttgctcgc
     3481 cagaaaacaa cccgtgttcg tctcgatgtc gccgctgagg actgtctggt tcgttgtgaa
     3541 gtcgcgatcg gccagtctga acgtggccgg ctcttcggtg ccggcaaaac cgccgtagac
     3601 cgagacgttg ttcttcaacg tgaaagtcgc tgcccgtcca ctcggcacac tgggcttgta
     3661 gtagtccgtc gggctcgggg acgcggccac ccagatgtcg tcgtgggtct gggccgcggt
     3721 caacgcggtc tgcaacgtgg tggcattctc ccaggttgtt ccgttgcccg agccttgggg
     3781 tttgacgtag atcacgttct cgcaggcatc gccgataccg tcattatcgt ggtccgactg
     3841 attcgggttt gagtcatccg ggcagttgtc gcaggtatcc agtatcccat cgccgtccga
     3901 gtcggccgtc ccgcaagatg ttcccgtccc ggcgggagtc ccgccattgg ccgcacacac
     3961 cgtgggcaag aggtcctgac aggagaagtc ggtcctgcaa caggcttcgg caatctcaac
     4021 gcttccggtg gtgctggccg tattgcccca ctggtccatg tcatccttgg cccgggcgag
     4081 ataacgattg gtttccaccg ggaaggatgc cgtggaaccc gtccaggtcc acccgccggc
     4141 cagactgctg tcgatgccca gcagcacgtc cgtgccgggc tcgatggttc cattgccgtt
     4201 cgtgtcgcga tagaactcaa ctgcggtcac gtaaccgtcg gcgtcggtca cgttgttggc
     4261 ggtcagggtc aggttgctgc cctgggcaac cgggtccggg ctatcggaca ggctgccgat
     4321 gctgggcttg gcgggagcca aatagctgat cgacaccgac gagtaggtgt ccggcccgca
     4381 gggtccgtca cagcttccgc catacgggca ggaagcgaac ccaagacccg cgcagtaggc
     4441 cgcaagagac gaattcatgg ccccagtcga ggtgacacga atggtcatcg tgttaccgct
     4501 cgctccgagc cagctattcc acgtttcggc ggggatggtc acggcgtgca gattcgccga
     4561 gagcggctct gtgccatcgc atggcgatcg cccagctttt gcacaggcgg ggcttgttcc
     4621 ggtgcaatcc aggttccact ccttgaagaa cttgccctca gcgggcatgt tggtgctatc
     4681 ggtgacaccc gggtccaacg cgtggttgtt ggcagtgacc gtcaggtatt tctcctccgg
     4741 gtccgcgtca cagcaaggga tgttggagca ggtatcgcag tcgccaagat catgcgtcga
     4801 gtcccagggg ttgcagccca ggtcggcaat ggcactgatg gtgatcgtga cgtcggtgcc
     4861 aacacgtgcg aggcattggt tcgagaaaat gtgtgtgttg gtgtcaccgc cgccaagtcc
     4921 cggcaccgca ggcggggttc ctcccggcgc gcagtcgcca aacagttctg tgcgcagcag
     4981 ggtcaacgac acgagcaggc agacgattgc gatcgcgcgt cgcttggtca tcttgagctc
     5041 cttcgagaag tgccgcttac acgccaacgc gacataatcg aaaccacgcc aagcacgaat
     5101 accgctgagc tcgtaggctc gggaacgact tccccccaca tctcccactt cgcctgatag
     5161 tcaagccgag atgctccagg atgcccaggt gagggtccaa tgctcaactg ctcgacactc
     5221 aatctcacga aatcgacacg ataccccgcg agatcagggg accgccccaa cagccaactc
     5281 tctggccctc cgctacctcc tcctccttct tcaaaaagtg ctagcacgga gaactggtcc
     5341 tcgattccat ttgtcacgag cccaacaagc ccattgaagc cggcagcatc ctcggtggtg
     5401 aaatccaccg ttccttgctg accatcctgc caatacacgc tgcccccaac tgtaaaagac
     5461 tggtaaggtg gagcgagcga gtacaactcc agcatcagac caacgcgcga tacatccgca
     5521 tagcggatgt cgtcgagtgc ctcccgggcc tcatatgatg atagcagtat tggggtggct
     5581 gtggcggcat tggccactgc gaacagaacg atgagcgaac gaatagcttg gtgaatcatg
     5641 aaaatctctc cgtcttagcc ctaaagagct aaccgtcatg ttacttttac ttgcttcggg
     5701 ctcgaattac gagtagcgca ctggcgcaac acagcagcga cgatgtcggc tccggcaccg
     5761 ggactccata gaactcatat ctacctgcag atatctccca gtctgtccag ataccatcgc
     5821 caccggggtc gactccgggg ctcctcaggt caactgtgac aacctctctg atggattcga
     5881 tccggtaccc gacccgatca ggaccgaatg aggggacgtg cagctcgaga tcgaacagat
     5941 cctgtccccc accccctccg agcaggcccg cattgttgtt ccacgctccc ttgagtttgt
     6001 cgtcgttgcg cccattcgaa agcaaggcag tgaattcgcc gaagtcagga tcacccgccg
     6061 agaagatggt gatgtcgatg tcagtgggga attgaggttg aatcgcaaac tcattgaaca
     6121 gcatcccacg aatgctattg tcctccatta gataaaagag gacaggccac gccgattcaa
     6181 cgacctgcgg cgtgcccagg gtgacgcgag catccagggt cgccaggaga atcggcgatg
     6241 ccttggcaac cttcattacc accaaaaaaa agaccagagt gccagataac ccgcgaatag
     6301 tcatgactca cctccctgta tgattgcaga caccgcatac gttgttaggt gcttagcgac
     6361 actgtcacat cgtagcttct cgcagatggg catgtcaagc actcgacatc aacacgttgt
     6421 ggaatgctgc aggcgcggtc cccggagtct atgtggagca ttgccttcga gaacacctgc
     6481 aattatccac gataacagcg atcacggccg gaaccgcaag cacagccaat gaagagggct
     6541 cgggaaccgg caggccgtaa aactcgtagg tgatctccgc agaatacgtg aatccctgaa
     6601 gatccgggtt gttcagcggg tcaggtgtcc atggctggaa gacgagattc tccacgatga
     6661 gccgcacttg tgtaagctcg tagccgaaga ggtctggtga gacgccgaac aacagtgact
     6721 ccggagcagg cgggctaatg tggccgcctc cagaggggaa cagatcccaa aaccggaact
     6781 cctccgcgat tccatttgtt gccgacaaag cgaagatcgc aaagccagga tcgccggcat
     6841 tggtaaagtc cagttggcct ggcgcctccc agaaggcccc aacgcctaga ccgtacttgt
     6901 cttcgagcga accgggtagt tggagaatga actcgacctg cggttcactc tctggcgctc
     6961 ctcctcccgc cacatctgaa gaatgtgatg ccaggagaat cggcgccgca tttgcagtgt
     7021 tcaatgccat ggacgcaacg gccatggcac aaatcaagga acgagcaatc atgacttcac
     7081 ctcctgcaga atcacgcgat ccatccgcac catcattagc gccgagaatg acatcctgct
     7141 tcataggatc ggatgataat tccagactgt cccagatgtc aagcagttgt cagcctgatg
     7201 ctgtgtgttg ccgtcatagg caagactttg gctggttctg ctcgggacgg tcaataccga
     7261 accggtccaa gctcatgcgt ctggcgttgc actgtcagcc aactctgcaa attgatgttc
     7321 gctacgccgc ctttgataag gtttcaccta caaggctgct gctattcgcg tccaatacag
     7381 gccacacacc accccaagcg atgcagacgg gtgtggcctt tttttcatgc gcatcgcaga
     7441 aaggagcatg ccatgcatgc aaacccattc gaaagccagc ttgaacggct ggcccttgac
     7501 gcgcggggga cgcgatgcgt ccggccttcg gcccacgggc accgcccgcc ccgtgtgtcc
     7561 agggctggcc cttgacacac cggaggtcgc tcgggcgacc gtgcgggacg cacccggcca
     7621 ccggccggcc ggtgagtcaa gggctggccg gccttattcc gttcgggtac taccgttctt
     7681 cttggccgga tcgcgcaccg gggacttcag ggccaggttg gcctcgagaa tatcgcccgg
     7741 cttgagcgac agcttcacgg acgccgcctt gaggccgggc ttcgttgctt tcaacgtgta
     7801 ttcgccgggt ttgaccgcga agtaccagta gacccccttg gtatcggtgg tcgccttctt
     7861 gtctgatgga gagatcgcaa cttgaacgtc tgctaccggt ttcccgctcg aatcggtaac
     7921 cttcccggtg atgaacgcgt gggtcggctt ccaggtcgat ttcatgtagt ccatcacctt
     7981 cgtgccgcgc gtctcccacc cttgccacat gtgcggcggg gcgccctgct ggaacaggaa
     8041 gacggccatc acgttctcgg ccgcggcgac cacttcgttc ttcgggtcgc cgtaccgata
     8101 gatctcgatc tgcttgatgt actcgtccgc tgtcagcttt tcgcggtcgc gccagccact
     8161 tcgtttctgc gggtcctcgc ccttgttcac cccgtcgctg ccaaactcgc cgatgaacca
     8221 cggcttgctc cgcaaccagg ggtagtcgtt catcgcccgg acatggcggt gggccagcca
     8281 cgtgtcgtag tgcttgaccg tcaggtccca gtactcgtgc gtctcgatcg cgtcggcctt
     8341 caggcaggcc tccttgacgt gaggtcgctc ccactccttg gagtccgtcg gacctcccgg
     8401 cccgtaggaa ccgaaaacga tcagcccctc gtaaccgccc ttgcgcaggg tgtcgtacat
     8461 gtcgatgaag cacctggctg tttccgggcc gacgtcgttg aactcattct ggaaaccgat
     8521 ggcgtcgggc cacttctcct tcggcaggtg cttcaggcgg tcgaggatct cttggccgaa
     8581 cttctttccg ccgatcttcg ggtcaccgtc cggcccggag gcgtggtccc acacccgata
     8641 gaagacccga gcgccgcgct ccttggcaat cgcgcaactc gtaatgtcga gcgccttgat
     8701 ccagggggca ccttccgccc atttcttcac gtcatccggc cagcccaata tgtgcggaca
     8761 gatcggtgct gcataaggct tctctgcccg cgatatcgac gcgcagaccg ccgacaccat
     8821 agccgccgcc accgccaacc tcattgctcg ccgcgccatt tcaccggccc cagatgtacg
     8881 attgttccaa cagctccagt tgaacacctt tcgagagaat acatcccacc gttctacacc
     8941 gcaacgcggg gaatgaagaa ccgtgaacat ggaaccacaa atggacggtg tcttgtccat
     9001 cttcgatgtc aaagcaggat tcaagctgat tacgttctgc ttttctgaga gacggagagc
     9061 tgacagccag gcctctcctt caatcggcac agttcgggtc ggcacgcacc cctggcccgc
     9121 tgaggcaacc acgaagtatc gagatgtcaa tgccgtcgat gacttcgtcg ttgttcacgt
     9181 ccgcattctg gcacctgggt tccgtctgtg tcgcgcctgt cccggtcagg caggcttgca
     9241 ggtgcccgaa gtcctcctgg tcgacgtcgc cgtcgtagtc gaaatcaggt gagacggttc
     9301 tcactgtcag gttgaccagg atcgtctgtg ggttgttggc agcctcctcg tcagagacgg
     9361 taatcaccgc cgtatactgc cccacctcca gcccggacac ctcatcgtag ctgatgtcga
     9421 tcgggtccgg ctcccccgtc gaatcgcccg ttgtgggcga ctggctcagc cagaaagcgt
     9481 catcctcgat cgtgtagttc agcgtaccga tgcccccgtt tcggatcgtg aacgtatcct
     9541 tgggcaggtc ctcaccaagg aagatcgtgc gattgaaaac ggtgtggctg agagtgatca
     9601 cgggcgccgg cacgtggatc acgacctcgt ccatgacctc gccgttatcc gccgcgacgt
     9661 accggagctt cagaaaccgc tggtcgacct cgaccagcat cggcccggcc cgccctccgt
     9721 tgttttcccc aaacgccctg gccagccagc ccggaagccg gttcggaacg ttcccatcaa
     9781 agggcatccc gcccggcgcg acaacgatct caatcacatg gtcgcccttg atatacagat
     9841 cccgattgac gaaaagcggg ctttggtcgg taaggtacgt gacgtcgccg cccgcatagc
     9901 cggtcagagg gaaactccgc tgataggtgt gaatatgccc gcaaagcagg acgtccgccc
     9961 ctgcctggac gaggacggga atcacctcgg ccgccatgtc gccctcggtg tcggggcgcc
    10021 cgacgcccga cttgggcgga taatgaccga caacgatctt ccagggttgg ctgctcgcgt
    10081 tgagatccgc ctgcagccac gtcacgatcg ccgactgccg cgcgtccgag ttgcccggcc
    10141 cgccccaagc cgtggcgtcg aaaatggtga aatgcgccag gccgtaatcg tacgagtagt
    10201 tcttctcggg cgactccccc acagggcctg ccaccgggct tgtgacaccc gaaaccggag
    10261 acggcacgtg gtagttgtcc agcgccggcc cgccgtcgtt ggtgtaggcc tcatggttgc
    10321 ccatgcagaa gtactcgacg tggttgcgga cgaacgccga ctgattcggg accagcgatt
    10381 cgttgaatcg gtaatcgtac tccggatgtt cgccgagatc ctggacgccg tcacccacga
    10441 gcatgacgaa tgccggatcc agggcagcaa tccgccccat gaccatttcg aataccggta
    10501 ccgtttcagg aagcctggca tgggcattgt cgcccgtcac cgcgaaactg aatggggcgg
    10561 tgtcgtcgtg gcgttttcgc gtgctgaacg caccggcata ggcggcaact accacggggt
    10621 tgcccggttc ccgcttatgg atcacctgat actcgtactg ctcgccgtag tcgagccccg
    10681 tgatcgtaac ccagtgattc acccgcgtgc ccgaaccggt cgccaacgtg ccgatcgggg
    10741 ccgccggcgt gagaccgggt tcgccgaccc gacgatatcc cacttcgaaa aaatcggtcg
    10801 gcgcgtcgcc cattccggat tccgttgtct gccagacgat aacgatctgg tccctctcgc
    10861 cgacgccggg ccccgtcgtc gccagcgggg cgtcgccgag ctgcagatac ggctggtgtc
    10921 cgacttcgag gatgcgccgg gcttcggcgg gcggcgacaa gaccaggata gcagcgcagc
    10981 aggcgactgc gatcgacgtc tcagcccgcc ttgacttcat cggcttgctc ctccgctctg
    11041 cacggaccat cgcccccaaa ccgccgccgc agcgctccga cgcctccggc ggccgcccga
    11101 tcgcgagcag acctcccttc ctattctacc acaccccctc cgcaactccg cagtcattcg
    11161 cagaacgtga gatcggcgca aacagaaccg ggaccaactg cacgatggtc ccggtcgtgg
    11221 agggcctctc atgtccggtt cgtcatcgtc tccgcctcag cagcgccggc aggccgcctg
    11281 ccatgagcag cgcggacaat gtcggttctg ggaccaccac gaggttgtcg agcaggtgga
    11341 agttgccctg gacctgtgct ccctcacgtg cccgcgtgta gataatcagc gaggcttcag
    11401 gaccggtcgc ggtgaaggtg tactggtacg tgtgccacag gccgtccccg aagttcgccg
    11461 ccgggaaggt gatggacccc ggaccgtcct cgatggcctt catgtcattg gcggacagtg
    11521 agccgtcggc ccgaaccgca tactgcgctc ggaaaggctt cgtcgtcagg ccgttcatgt
    11581 tgcccaactt catgtcgaag aacacgttgt actcaacgcc tgcaacagta ggaaacttcg
    11641 tccccacgta gatatcacgc ggattcatct gctcgtcgtc gatcatgatc ttcatcgagc
    11701 actcgccttc ggtcttgtcc gccggttcct tcgacagccg ccagtgaacg gcggccgggc
    11761 cgatccaact gggataggcc acgtaccagt cgggagcaat cgagccggcg aagttcagtt
    11821 cgctgtcgtc cgtgtacggc ggctgcatcg tgcccccgcc tgttcccgcc cctgctctct
    11881 gatcgagctc cgccgatgca gcaagatacg tgccctccaa cccgaggttg acggcttgga
    11941 cggtaccggc cagaaccgca ccgaccacga tggcttgcac aatcgtcaaa agcctcatca
    12001 ctcgcctcct ttaccgtaat cgtgttgccc tggaatggag aagtcttcgc gccccgtgcg
    12061 aatccgcacg aacacgcaca agttctctct ctgcctgatt tgtcagctac gatcatatca
    12121 ccaaaggctc ttgtgtcaag caaaaacacg aaagacttgg ccgcagatta aagcgacagt
    12181 ggatacgtgg gctcccccgc ttttgcatca tcgtcggcaa acctatgccg gccccgctct
    12241 atactgtgcg gaacggggcc gccccgcctg caactgcttc caccctgccc ttcttgcgtg
    12301 cgaccgagga atgaatcctc ggccgcaagc ggcgcagggc ccccgctcgc ggaccattcg
    12361 agctgctcgg cgaagaacaa aggctgagaa ggtcaaaggg cagaggctaa cgtcgttaca
    12421 cacttttcct tcggaagact cccatgacgg ctagcggacc tttccgactt atcctaccgc
    12481 ttctcatatt tctgaacagg ctgg
//
LOCUS       DAOTPW010000170        12367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4911_length_12367_cov_5.237086, whole genome shotgun sequence.
ACCESSION   DAOTPW010000170 DAOTPW010000000
VERSION     DAOTPW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12367
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4911_length_12367_cov_5.237086"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1750
                     /locus_tag="P5316_14840"
     CDS             191..1750
                     /locus_tag="P5316_14840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRR86282.1"
                     /translation="MFEYMGQCILAGRTLYVDCWDNKPPLLPWVNAAVLALTNDSTVA
                     ITVAAGMAAAACVACVAVGVWICFGPHVAGATALLAAFVISLRRNEGCTNGTELYATL
                     FESLAALCLLLAWRATKRRLTAWGLVGGMALALAFTAKQHALGGLIALGVVTLIETIR
                     RRGFRHPLLRILLAACTGFVAVIGCVCAVLRAQGSLGEAVFAVFTSNLVFVRNGHSWF
                     VPVDYQASKLTSQLEPLWPIIWLAGIGLLASVVRWRRPDPDEQIGPQHLPNDLVLLLT
                     VWLGFGAWTVTWGPAHIPRYWHATYVPLFWLSAQTFSFLRPMLTIRPLHVRGLTMVVT
                     LTAGWLMFRPALEIYQFDMKRSIYYAYEDPDRDCLQVIAAEVVRRTAPTDEIYMWGFE
                     PGVYRFSHRFSPSRFFELGKLHALGANAEFLVHEVVETLRARPPRLVLIRRTDYSEVQ
                     SGSLAGLDIRGFADLLAREYDPAGLVRDVIFFERRPPGSDTAAPARHIATPSKPVETA
                     SPSSMAAPVHR"
     gene            complement(1777..5259)
                     /locus_tag="P5316_14845"
     CDS             complement(1777..5259)
                     /locus_tag="P5316_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRR86283.1"
                     /translation="MTHDQGSKQHTPSASPSSGRPAGRHHGFTNAIVHVFLHSNLSLI
                     LTIIAAAMGLAALLMTPREEDPQIIVPLADVIVNCPGRSSQEIEQLVATPLEKILYQI
                     DGVEYVYSVSRENMALITVRYFVGEDRERSLVKLYKKMDESIDMVPPGVTGWVVKPVE
                     IDDVPIVTLTLTSATSDEFVLRRVGEELVERLAATKDVSRAYVIGGRSRIIHVYLDPE
                     RLQAHQLDPLAVRHAIGAANVTQTAGNYTRDDQVVQVEAGLAFARPEELDELVVGVFD
                     GRPVFLRNVARIVDGPAEADNYVRHGWGPAAGKLHDRHAGGAVIGPASRNGNTNSSDP
                     AVTIAIAKKKGSNAVWVARDVIAAAEALKTEVIPDDIDLVITRNAGLTSNEKVNELVE
                     ALAVAVLFVMVVLTLGLGWRESLIVAVAVPVVFGLTLAVNMMFGFTINRVTLFALILS
                     LGLLVDDPIVDVENISRHFALRKRAARHIVLEAVAEIRPPLITATLAVIVSFLPMFFI
                     TGMMGPYMRPMALNVPVAMLMSMVVAFTITPWLSYHVLGRHYNKAGAAHTEGPEDDLD
                     SETIKKSPLYRLFHPLMAPLLHKRWLSWTFLGAMGALTVGAMMLAVTRHVPLKMLPFD
                     NKNELQLVLDFDEGTTLERSDAAVRDFEDFLRTVPEVTDFTSYVGIASPMDFNGLVRQ
                     YYMRRGDNVAEVRINLVGKKSRKQQSHGISLRLRDDLTGIASRHNASLKIVETPPGPP
                     VIASIVAEVYGRPANTYEDLIAAARTVRTRMEMEPGVVDVDDTVEADSKKLIFVTDKE
                     KAALNGITVAQITETLRTALEGASAGTIRTRGERHPLEIVLRLPRAIRSDLFDLSKLH
                     VRGEKGQLVPLAELGRIERSVVDKSIYHKNLRRVVYVFAETAGRPPADCVVDIQSDKM
                     PGAIALEPPPQGVDHSHGFVKKADATRPVAGRTFLSNGSGIKWWLPDGYDVDFAGEGE
                     WKITLDVFRDLGLAFVAALIGIYILLVGQTKSFVIPVVVMLAIPLTVLGIMPGFWLLN
                     LVAGSHVGQYADAIFFTATGMIGMIALAGIVTRDSIILVDFIHLSLAKGRSLFDAIME
                     SRVVRLRPILLTAGTALLSALPITIDPIFSGLAWSLIFGLVASTIFTLFVIPVTYWLL
                     YANKPGHGLPATEE"
     gene            complement(5270..6394)
                     /locus_tag="P5316_14850"
     CDS             complement(5270..6394)
                     /locus_tag="P5316_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR86284.1"
                     /translation="MATVLPSCKYRRVAKTIIISIAAVTFVVVTMMWLMGYFHPKLAG
                     APTASVGRPLGDVQVVPVEKIRVPVTESAVGTVRPVHESAVASKIMAKVVAVNVKAGD
                     RVKKDDVLIKLDDADLKARQQQALAAVAAAKAARDHATIEFDRVKRLMGQNAASRIEY
                     ERAETALKSAEAELQRAEQTLNEAETVLSYATIHAAMDALVVDKRVDVGDTVVPGQVL
                     LNLYDPSRMQLVASVRESLTRRLQVGQNIEVTIDALGHPCEGQISEIVPQAESASRTF
                     LVKVTGPCPPGVYAGMFGRLTIPLDEEDILVVPREAIRVIGQLSVVDVVDGKMLQRRV
                     IQPGRTLGGKLEVLSGLREGEQIALTRVSAGESTESRPNV"
     gene            complement(6518..8203)
                     /locus_tag="P5316_14855"
     CDS             complement(6518..8203)
                     /locus_tag="P5316_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR86285.1"
                     /translation="MSSKKIVIVGGVAAGASAATRARRLNESAHIVVFERGEYISFAN
                     CGLPYHIGGQIADRGKLLLADPRSLKRTSNIEVHTGCEVLSIDRRTKHVEVLDRRSGR
                     RWREPYDKLILAPGASPVLPSWSGIDRRNVFVLRDMTDMDRINAVLDKGEAKRATVIG
                     AGYIGIEMVEALTERNLEVTLIEQQAQVLPLMDVEMAREVARVLRLHGVEVILAASVE
                     SLKTDGDRVVAVQLADGREVATDLLLVSIGVRPNASLAEEAGLAIGESGAIAVNAQML
                     TSDADIYAVGDAGEVIFAVTGEPGRFPLAGPANRNGRNAGGHAAAGRSFSAAPVAGTS
                     ILEVFGLAAGMTGLSAKGANRCGIPADVAYAVRGHHVGYYPGAKEIILKLIYDPESGR
                     ILGGQAVGKAGIDKRIDVIATAIHFRGTVDDLAGLDLAYAPQFGAAKDPIHIAAFVAQ
                     NKREGLLAEILPGEEPIGDQLVDVRSEQEFAAGALPGAINIPLPDLRQRMDELDRSRP
                     VMLNCGVGERSYKAARVLMQSGFREVYSLAGGYRLHRDTWKPPVHSNTRGGAA"
     gene            complement(8321..8638)
                     /locus_tag="P5316_14860"
     CDS             complement(8321..8638)
                     /locus_tag="P5316_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRR86286.1"
                     /translation="MIEKDQRLIDMKVLEQSAAVMRVLAHPHRLRICELLMAGRVSVG
                     DLARQLGIASNAVSQHLNMMKAHGLLTCEREGKAVYYGVCDPRPAWLLQCIRNHACGK
                     QRL"
     gene            complement(9339..9734)
                     /locus_tag="P5316_14865"
                     /pseudo
     CDS             complement(9339..9734)
                     /locus_tag="P5316_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
     gene            complement(9721..10722)
                     /gene="ispE"
                     /locus_tag="P5316_14870"
     CDS             complement(9721..10722)
                     /gene="ispE"
                     /locus_tag="P5316_14870"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRR86287.1"
                     /translation="MTRVSVTVRSPAKINWFLRVLGRRQDGFHDIRSLLSAITLYDEL
                     ALASRDTSGIELVCDHPDLPTDHRNLIVKAGKLLAEAAGIEPRATCRLRKEIPIGGGL
                     GGGSSNGAASLVAFNRLWGLDWSVEQLAPLAAKLGSDVPFFLRGGSAVISGRGERIRP
                     VRLQWEGWIVLIFPPFGVSTAEVYGAWRPPASPPAPTLLDEMPAGVDVLRLDSRQLMA
                     AAYNMLEQPLLLVRPQVGPLLDMLKRLAGRPVRVSGSGSTLFTAYDNYAEAMGFARRV
                     EDETGMRARLAQLGKPGVWEADSAAGEAFEPGEASPRECLGCFREDRQLEGGRDGNH"
     gene            10938..11075
                     /locus_tag="P5316_14875"
     CDS             10938..11075
                     /locus_tag="P5316_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86288.1"
                     /translation="MCDFDLGVRVVAETDRALADMTYLKGVDHDAIQFALSRHKVALL
                     F"
     gene            12192..>12367
                     /locus_tag="P5316_14880"
     CDS             12192..>12367
                     /locus_tag="P5316_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRR86289.1"
                     /translation="MRPCPAGPYEPTWESLDARPNPAWFNDAKFGIFIHWGVYAVPSW
                     SPKGQYSEWYWHSMG"
BASE COUNT         2338 a         4086 c         3455 g         2488 t
ORIGIN      
        1 cgcgctgttt atccatgtag tgggcagcac caccaaacca acctccggcg tcgcagctcc
       61 gtaagggcaa aggcgtgctg tgaaatctct tgtcaacatc ctgtcggctc ttgcgctcct
      121 cggctcggtg gtcctgatcg tctcaggcag ttgggccgca caccactggc ccgcctccca
      181 ggacagacac atgttcgaat acatggggca gtgcatcctg gccgggcgca cgttgtacgt
      241 cgactgctgg gacaacaagc cccccctgct cccatgggtg aatgcggccg tacttgccct
      301 gaccaacgac tcgacggttg caatcacggt tgctgcgggc atggcggcag cggcctgcgt
      361 cgcctgcgtg gccgtcggcg tctggatttg cttcggtcct cacgttgccg gcgcgaccgc
      421 cctgctggct gcgtttgtca tctcgttacg ccgcaacgaa ggctgcacca acggaacgga
      481 actctatgca accttgttcg aaagcctcgc cgccttatgc ctgctcctcg cctggcgggc
      541 cacgaagcgc cgcctcacgg cgtggggact tgtcggcgga atggcgttgg ctctggcatt
      601 cacggccaaa caacacgcgt taggaggcct gatcgccctc ggtgtcgtca ccctcatcga
      661 aaccatccgc cgccgcggct tcaggcaccc ccttctgcga atcttattgg cggcgtgcac
      721 ggggtttgtg gccgtcatcg gttgcgtttg tgcggtgcta cgcgcccagg gcagcttggg
      781 tgaagccgtc ttcgcggtgt tcaccagcaa cctggtattt gtcaggaatg ggcacagttg
      841 gtttgtgccc gtcgactatc aggcatccaa gctgacctcg cagttggagc ccctctggcc
      901 gatcatctgg ctggcaggca tcggtttgct tgcgagtgtc gtccgatggc gacgccccga
      961 tcccgatgag caaattggcc cgcaacacct tcccaatgat ctggtcctgc tgctgaccgt
     1021 gtggctcggg tttggtgcct ggacggtgac ctggggaccg gcacacatcc cccggtattg
     1081 gcatgcaacc tacgtgccgc ttttctggct gtccgctcag accttctcgt tcttgcggcc
     1141 gatgctgacc atcaggccgc ttcacgttcg gggactgacg atggtcgtca ctctgacggc
     1201 cggatggctg atgttccggc cggcgctgga gatttaccag ttcgacatga agcgcagcat
     1261 ctactacgca tatgaggatc ccgatcgcga ttgccttcaa gtcatcgctg cggaagtcgt
     1321 tcgcagaacg gctcccaccg acgagatcta catgtggggc tttgagccgg gagtctatcg
     1381 tttcagtcac cggttctcgc ccagccgctt cttcgagctt ggcaagttgc acgccctcgg
     1441 cgccaacgcc gaattcctgg tccacgaagt cgtggaaacc ctgcgtgcca gaccacccag
     1501 gctcgtcctc attcgtcgta ccgactattc ggaggttcaa tccggctccc tggccgggct
     1561 cgacattcgc ggtttcgcgg acctgctggc tcgcgaatat gatcccgcag gccttgtgcg
     1621 cgatgtgatc ttcttcgagc gtcgaccacc tgggtcggat accgccgccc ctgcgcgaca
     1681 cattgccacc ccgtcgaagc ccgtcgagac ggcgtcaccc tcctcaatgg cggcgcctgt
     1741 ccaccggtga tcggcgccat ccaagaagag cgtttctcac tcctccgttg ccgggaggcc
     1801 gtgaccgggc ttgttggcgt acaagagcca gtaggtcacg gggatcacaa agagcgtgaa
     1861 aatcgtcgag gcgaccagcc cgaaaatcag cgaccacgct aggcccgaga agatcggatc
     1921 aatggtgata ggcagggccg acaacaaagc cgtgcccgca gtgagcagga tcggacgcaa
     1981 tcgcaccaca cggctctcca tgatcgcgtc gaacaagctg cgccctttgg ccagcgacag
     2041 atgaatgaag tccaccagga tgattgaatc gcgcgtgacg attccggcca acgcgatcat
     2101 gccgatcatg cccgtggcag tgaagaagat cgcgtccgca tactgcccca catgcgatcc
     2161 ggcgacaagg ttcagaagcc agaagccggg cataatgccg agaaccgtca atgggatggc
     2221 cagcatcacg actaccggaa tgacaaaaga cttcgtctga cccacgagca ggatatagat
     2281 gccgatcaga gcagccacga aggccaagcc caggtcgcga aaaacatcga gcgtgatctt
     2341 ccattcacct tcgccggcaa agtcgacgtc atagccgtcc gggagccacc acttgatgcc
     2401 gctaccattg gagaggaacg tgcgacccgc gaccggacgg gttgcatccg ccttcttgac
     2461 gaagccgtgg ctgtgatcca cgccttgcgg cggaggctcc aacgcgatgg ccccgggcat
     2521 cttgtccgac tggatatcga ctacgcaatc ggccggcggc cggccggcgg tctcggcaaa
     2581 tacatagacc acgcgcctga gattcttgtg gtagatgctc ttgtcgacca cgctccgctc
     2641 aatgcgccct aactcagcaa gaggaacaag ctgccctttc tcaccgcgaa catgcagttt
     2701 cgacaggtca aacaaatccg agcgtatggc cctgggcaac cgcaacacaa tctccagggg
     2761 atgtcgctcg cctcttgtac ggatggtccc ggcactggcg ccctcaagtg cggtccggag
     2821 cgtctccgtg atctgggcaa ccgtgattcc attgagcgcc gccttctcct tgtccgtgac
     2881 aaagatcagc ttcttgctgt cggcctctac ggtatcatcc acatcgacaa ctccgggctc
     2941 catttccatc cgcgtgcgaa cggtccgggc agcggcgatc agatcctcat aggtgttcgc
     3001 cggccgccca tagacttcgg ccacgatgga ggcaatcacc ggcgggccgg gcggcgtctc
     3061 cacgatcttc aggctggcat tgtggcgaga ggcgatgccg gtcaggtcgt cccgcaatct
     3121 caggctgatg ccgtgactct gctgcttccg gcttttcttc ccgaccaggt tgatccgcac
     3181 ctccgccacg ttgtcgccac gacgcatgta atactgtcgc accaacccgt tgaaatccat
     3241 gggtgaggcg atacccacgt agctggtgaa gtcggtgacc tcgggcaccg tgcgcaggaa
     3301 gtcctcaaag tcccggacgg ccgcgtccga ccgctccagc gtcgtgccct catcaaaatc
     3361 gagcaccagt tgaagctcat tcttgttgtc gaacggcaac atcttgagag gcacatgccg
     3421 cgtgaccgcc agcatcatcg cgccgaccgt caacgccccc atggcgccta ggaaagtcca
     3481 cgacagccat cgcttgtgga gcaatggggc catcaggggg tgaaagagac gatacaacgg
     3541 cgacttcttg attgtctcgg agtcgagatc atcctccgga ccttccgtgt gagcggcccc
     3601 ggctttgttg tagtgtcttc ccaagacgtg gtacgacaac cagggagtga tcgtgaacgc
     3661 cacgaccatc gacatcagca tcgcgacggg aacattcagc gccatggggc gcatgtacgg
     3721 gcccatcatg ccggtaatga agaacatcgg cagaaacgag acgatgacgg ccagcgtcgc
     3781 cgtgatcaac ggaggacgga tctcggcgac cgcctccaac acaatgtgcc gcgcggcccg
     3841 cttgcgaagc gcgaaatgcc gcgagatgtt ttcgacgtcg acgatcggat cgtcgaccag
     3901 cagacccaac gacaggatca gggcgaacaa cgtcacccgg ttaatggtga agccgaacat
     3961 catgttcacc gcgagcgtca ggccgaacac caccggcacg gcgacggcca cgatcagcga
     4021 ttcccgccag cccaaaccca gcgtcaggac caccatcaca aacagcaccg ccaccgccag
     4081 tgcctcgaca agctcgttga ccttctcatt tgaagtcaag cccgcattgc gggtgatcac
     4141 caggtcgatg tcgtcgggga tcacttccgt cttgagcgcc tcggcggcgg cgatgacatc
     4201 cctggccacc cagacggcgt tggaaccctt cttcttggcg atcgcaatag tgaccgccgg
     4261 gtccgaggaa ttcgtgttgc cgttccgcga agcaggcccg atgaccgcgc ctcccgcatg
     4321 tctatcgtgc agcttgccgg cggccggccc ccacccgtgc cgaacatagt tgtccgcctc
     4381 ggccggtcca tcgacgattc tggcaacgtt gcgcagaaag accgggcggc cgtcgaaaac
     4441 cccaaccacc agttcgtcca actcctcggg tcgggcgaat gccaaaccgg cctccacctg
     4501 cacgacctgg tcatcgcgag tgtagttgcc cgcagtctgt gtgacgttcg ccgcgccgat
     4561 cgcgtgtcgt accgccaatg ggtccaattg gtgggcttgc aacctttcag ggtcgaggta
     4621 aacgtggata atcctggacc gccccccgat gacgtaggcc ctcgatacgt ccttcgtggc
     4681 ggccagtcgt tccaccagtt cttcgcccac acgacgcagc acaaactcgt cggaggttgc
     4741 gcttgtcagc gtcagggtca cgatcggaac gtcgtcgatc tcgacgggct tgacgaccca
     4801 gccggttacg cccggcggca ccatgtcgat gctttcgtcc atcttcttgt agagcttgac
     4861 cagacttcgc tcgcggtcct ctccgacaaa gtagcggacc gtgatcaggg ccatgttctc
     4921 gcgcgacacg gagtagacgt attcaacacc gtcgatctga tagaggatct tctccagagg
     4981 cgttgccaca agctgctcga tctcctgcga cgagcggccg ggacagttca cgatgacgtc
     5041 cgccagcgga acgatgattt gcggatcctc ctcgcgcggc gtcatcagca gcgccgccag
     5101 gcccatggcc gcggcgatga tcgtcaggat cagagaaagg ttcgagtgca ggaaaacgtg
     5161 gacgatcgcg tttgtgaaac catgatgccg gccggccggg cggccactcg acggagatgc
     5221 cgatggagtg tgctgcttgg atccttggtc gtgtgtcatt gcacggccct catacgttcg
     5281 gcctggattc ggtcgactcc cccgcagaaa cccttgtcag ggcgatctgt tctccttcac
     5341 gcagtccgga gagcacctca agcttgccgc ccagcgtccg gcccggctgg atcacgcgtc
     5401 tttgcagcat ctttccgtcc accacgtcaa caacgctcaa ctggccgatc acgcggatcg
     5461 cttcacgtgg aacgaccaga atgtcctcct cgtcgagcgg aatggtcagc cgtccgaaca
     5521 tgcccgcata aacgcccggc ggacacggtc cggtcacctt caccagaaac gtgcggctgg
     5581 ccgactcggc ctgaggcacg atctcactga tctgcccctc gcacggatgc cctaaggcgt
     5641 cgatggtcac ttcgatgttc tgcccgacct gcaaccgtcg ggtcagcgac tcgcgcacgc
     5701 tcgccaccaa ctgcattctg ctcgggtcat agaggttcag gagcacctgc ccgggcacga
     5761 ccgtgtcacc cacgtcgaca cgcttatcca ccaccagggc gtccatcgcg gcatggatcg
     5821 tcgcatagct taatacggtt tccgcttcat tgagtgtctg ctcggccctc tgcaactccg
     5881 cctcggccga tttcagcgcg gtttcggccc gctcgtattc gattctcgat gcggcgttct
     5941 gtcccataag cctcttcacc cggtcgaatt cgatcgtcgc gtggtcccgg gccgcctttg
     6001 cagccgccac cgccgcaaga gcctgttgct gacgagcctt caggtcggca tcatcaagct
     6061 tgatcagcac gtcgtccttc ttcacacgat ctccggcctt cacgttcacc gccacaacct
     6121 tcgccatgat cttcgatgcc accgccgact catggacggg acggacggta ccgaccgccg
     6181 attccgtgac aggcacgcga atcttctcga ccggaaccac ttggacgtct ccaaggggac
     6241 ggccaacaga ggcggtcggc gcccccgcga gtttgggatg aaagtacccc atgagccaca
     6301 tcatcgtcac gacgacgaac gtcaccgccg caattgaaat gatgatggtc ttcgcgacac
     6361 gccgatattt gcatgaaggc aaaactgttg ccacgagaaa tactccttgt tccgtgcgaa
     6421 cccgcgcctc ccgtccgtct gattcgatct tttcgtaaac ggcccgttca cgctttgggc
     6481 atatcggaac gcgtgttatc gacaaacccg aggatggtca cgccgcccca cccctcgtgt
     6541 tactgtgcac gggcggtttc catgtgtcgc gatgcagccg atatccgccc gccagactgt
     6601 acacctctcg aaatcccgac tgcataagta cgcgagccgc cttgtaggat cgctcaccga
     6661 cgccgcagtt gagcatcacc ggccttgatc ggtccaattc gtccatccgt tgacgtagat
     6721 ccggcaacgg gatgttgatc gcaccgggca gtgccccggc tgcgaattcc tgttcgctgc
     6781 gcacgtcgac cagttggtcc ccgatcggct cttcgccggg cagtatctca gccagcaggc
     6841 cttctcgttt gttctgggct acaaatgcgg caatgtggat cggatccttg gcggcgccga
     6901 actgcggagc atatgccaga tcgagtccgg ccagatcgtc caccgtgccg cgaaagtgaa
     6961 tcgccgtagc aatcacatcg attcgcttgt cgatccccgc cttaccgacg gcctgtccgc
     7021 ccaggatgcg gccgctctcc ggatcgtaga tcagcttgag aataatctcc ttggcacccg
     7081 gatagtagcc gacgtgatgg cctctgacgg cataggcgac gtctgccgga atcccgcaac
     7141 ggttggcacc cttggcgctc aggcccgtca tccctgcggc gagcccgaac acctccagaa
     7201 tggacgtccc ggccacaggg gctgccgaaa aggagcgacc cgccgccgcg tgaccgcccg
     7261 cgttccggcc gttccgattt gccggcccgg caagcggaaa ccgtcccggc tccccggtta
     7321 ccgcaaaaat gacttctccg gcatcaccga cggcatagat gtccgcatcg ctggtcagca
     7381 tctgcgcatt gaccgcgatc gccccgctct cgccgatcgc caagcctgcc tcttcagcca
     7441 agctggcgtt cggacgcacg ccgatcgaaa cgaggagcag atccgtcgcc acctctcgtc
     7501 catccgcgag ctgcaccgcg acaacgcggt caccatcggt tttcaaactc tcgacgcttg
     7561 ctgcaaggat aacttcgact ccgtgcaagc ggagcactct cgccacctcg cgagccatct
     7621 caacgtccat caagggcaac acctgagcct gctgctcgat aagcgtgacc tccaggttac
     7681 gttcagtgag tgcctcgacc atctcaattc cgatgtagcc ggccccgatc accgtggccc
     7741 gcttggcctc gcccttgtcc agaaccgcgt tgatgcgatc catgtcggtc atgtcgcgga
     7801 gcacgaaaac gttccgccgg tcaatgccac tccaggacgg gagcacggga gacgcgcccg
     7861 gagcaagaat gagcttgtcg tagggctcac gccaccgacg accgctccgg cgatcaagca
     7921 cttccacgtg tttcgtcctt cgatcgatgc tgagcacctc gcaccctgtg tgcacctcga
     7981 tgttggacgt ccgtttgaga gaacgcggat ccgccaacag cagcttgccg cggtcggcaa
     8041 tctgtccgcc gatgtgataa ggcaaaccgc agttggcgaa ggagatgtat tctcctcgct
     8101 cgaaaaccac tatatgcgcg ctttcattca aacggcgggc ccgggtcgcc gcactcgcgc
     8161 cggccgccac gcctccgaca ataacgatct tctttgaact catctggttt cgcctcagcc
     8221 cgtgactcct tgaatgatgc ctcggcatct gatcttgcct gtcgaacccc caccgtctcg
     8281 ccggttcaag acctgttggt tccgcgccga acagcctggt tcacagtcgt tgcttcccgc
     8341 aagcgtggtt gcgtatgcac tgcaggagcc acgctggacg cgggtcgcaa acgccatagt
     8401 agacggcctt tccttcccgt tcgcacgtca gcaatccgtg agccttcatc atgttcaggt
     8461 gctgactgac cgcgttactg gcgatcccca gttgccgggc aagatcgccc acggaaaccc
     8521 gcccggccat cagcaactcg caaatgcgca gcctgtgcgg gtgggccagc acccgcatga
     8581 ccgccgcgct ttgttcaagc accttcatgt ctatcagccg ctgatccttt tctatcatga
     8641 tatcattgta tcatcatgtt tcgatttgtc aatatgaatg tgtaactcgt ttcccctcag
     8701 catcttaagc gtgattccat atgttcgcag tcgcccaaag agagccctct gaatcgctcg
     8761 atggtgttcg ctccccacca tcctggctgc gcgagggcag gcgcctgatc tcaattgagt
     8821 cctgctccga aaacgtcgtc agccttctgg tcctcgggct gcggcgctga ggcttgggga
     8881 acaacaagcg gcggctcggc cggttggctt gagatcggtt cgcctgagac tcttgccggc
     8941 tcggatggct tttcggccgg ttctgccgag ccggccccac gcctgcgatc gtttcggtga
     9001 tggtcgggac ggcggtcgtt tccaccatcg gcggacgacc gttgttgccg tggcgtgccc
     9061 gactgcccca cagcaatacg ggcccccggc cgaggataag cgccatgcct gtcctgccgg
     9121 cgaccctgtc gcgaagccgc ctctttcttc aaatccgcga tgagagaatc gtagtcgctg
     9181 aaaactgatt cgctcttctg tgcatcgtgt tccagggatc cggcctggtc gcccgtgtcg
     9241 gcctcgtctc ttgagccggc accgatttgc gactcggttg tttccgttgc gtcgagatct
     9301 tcgatgaaag cttcgtcgat ctcgtcaaac ttgggcggcc ggtagcctgg cagtttggat
     9361 cgctccaatt cctcgtggta ggccttgatc actgccgcct gaatcatctc tctgcattcc
     9421 gtgttgatcg gatgagcaac gtcggcatgg atcttcgcgc ggccgtgcga gttccgtgca
     9481 gtgcggcgat cgccaagcga atgaccgcac tcgttacaga acttggccct tagatggttc
     9541 ttcgaccggc atcgcggaca gcggtcacag agcttgcgcg agggcatggc gacaaagacg
     9601 ctgtcgccgg caccgctccc gatgatcttc agatcgcgta tgacaaacgc gccgtcaaag
     9661 gtgatgctgc aaaaagcccg aagtctttcc ctcgatttct ccaccagttt gacccgaacc
     9721 tcagtgattt ccatctctgc ctccttcaag ttgtcggtcc tcgcggaaac aaccgagaca
     9781 ttctcgcggc gaagcttcgc caggctcgaa cgcctcgccg gccgccgaat ctgcctccca
     9841 aacccccggc tttcccagtt gagccagccg ggcgcgcata cccgtctcat cctctacgcg
     9901 gcgggcaaag cccatcgcct ccgcatagtt gtcatatgcg gtgaaaagag tcgacccgct
     9961 gccggacacc cgcaccggtc gcccggccag ccgcttcaac atgtcgagta gcgggcccac
    10021 ttgcgggcgc accagcaaca agggttgctc cagcatgttg tacgctgccg ccatcaactg
    10081 tcgcgagtcc agacgaagaa cgtcgactcc tgccggcatc tcgtccagga gcgttggggc
    10141 aggtggactt gccggcggtc gccaagcccc atagacctcc gcggtcgaca cgccaaaggg
    10201 cggaaagatc agcacgatcc atccttccca ttgcagcctc accggcctga ttctctcgcc
    10261 acggccgctt atgacggctg atcctccccg aagaaaaaaa ggaacatcac tgccaagctt
    10321 tgctgccaac ggagcgagct gttcaaccga ccaatccagg ccccagagac gattgaaagc
    10381 cacaagcgag gcggccccgt tagagctacc cccgccaagc ccgcccccta tcgggatttc
    10441 tttacgtagg cgacatgtcg cccggggttc gatgcccgct gcttcagcca ggagcttgcc
    10501 cgcctttacg atcagattcc tgtgatccgt cggcaaatcc ggatgatcgc acaccagctc
    10561 aatgccgctg gtatcacggc tcgccagagc caactcgtca tagagcgtta tcgccgacag
    10621 cagggacctg atgtcgtgaa acccgtcttg acgccggcca aggacgcgca gaaaccaatt
    10681 gatctttgct ggcgaccgaa ccgtaacgga aacccgtgtc acgggcaggc atccttgccg
    10741 aggcaggtca gcgcctcgcc gactacccca ggaaacacac gacgacaaca aaccattcag
    10801 cgaacttcca tccttccgca tggcctgcaa gcgaatccac cgccccggcc gtaccatcgg
    10861 gtccaccaca gtctccgcac acaacaattc agcgattgat ccccaaagta cgcccgcaaa
    10921 tccttgtgga gacacacatg tgtgacttcg acctgggagt acgcgtcgtg gcagaaaccg
    10981 accgggcact ggcggatatg acttatctca agggtgtcga tcatgatgca atccaattcg
    11041 cgctgtctcg tcacaaggtt gcgttgttat tttaggatat gtaccgttcc tgtcaaccgg
    11101 tccgctaacc gggtgatccc aacacgcctt gtcgccggtc cggcagaagt cccgaggcct
    11161 gcctgatgtc ccacgccctg ctgcttggct ccccgtcggc cgacccgcct cttgggcccg
    11221 ggaacgtcgc cgtttcgaag ggccaacccc tcagcgggat cacccttgtg agtttctgcg
    11281 cggaccggtg ctggccgagg ccgattttta gcttatgacc tggcattgat cacccgatct
    11341 gaattgtggc gatgccgttt gtatggaccc gttcccgagg cctttggggg ctatggcgat
    11401 tacgattatc tgccccaacc tgacctgccg cagcgtgctc caggtttccg acagcatgcg
    11461 gggacagaag gtccgttgcg cgcggtgcgg taggaatttc cgcgttccgg attcgacggg
    11521 aaacaagggt agtcaggcca aagcacccgc ggaaacacaa agcggctcat caaagtgact
    11581 cgcctcccca atgtccgata agtgacctgc ccgatgaagg ttggctgccc gttttctccg
    11641 ccagtaaccc actggggttg tgtgacgcgc ttgccccgcc caggtcgtgc tgcggccctc
    11701 gagttcccaa caggcccggt tctcctctca tcttctaagg cggaccgcct ttctgcaggc
    11761 cgcaacccaa gcgctctgcg atccagaaac atgcgcgcaa gccgcacaga tgtctggccg
    11821 taaggttctg atgccaagcc cgctccctcc tcacgccgag cgttgcagcc gtcataacgt
    11881 actcgggtga gggggcaact gtccgaactc cgcggttctg tgtttctgtt cctccttcgg
    11941 ccacaattgc ccgattacgc cagagcactt ccattgggtt cgccccgccc tccatgcagt
    12001 cgctcctcgt cggcaaaagc cgattacccc ccctccaacc ccgtcttgcc gacacggttg
    12061 ccaagcctgc ggggatccgg ataatacctc gccaatgacg tgaacccgaa ccgcatccgt
    12121 tttcgcttct gaggaaaccg atcatgaaac atgtcgtttg tgtcgcctcg gttgttggaa
    12181 tcctgtctgc gatgcggccc tgcccggcgg ggccttacga gcccacatgg gaatcgctgg
    12241 atgcacgtcc caacccggcc tggttcaacg acgccaagtt cggcatcttc atacattggg
    12301 gtgtctatgc cgtgccttcc tggagtccca agggccagta ctctgaatgg tactggcaca
    12361 gcatggg
//
LOCUS       DAOTPW010000171        12335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4922_length_12335_cov_5.101954, whole genome shotgun sequence.
ACCESSION   DAOTPW010000171 DAOTPW010000000
VERSION     DAOTPW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4922_length_12335_cov_5.101954"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="P5316_14885"
     CDS             <1..1140
                     /locus_tag="P5316_14885"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRR86290.1"
                     /translation="TEWGGEIDVVKTSAVTGQGVPELIEHLGMLTELLNLRADPDVPA
                     TGTVIEAQMTQGVGPLARVLIREGTLRPGAFIVCGSAAGRVRVMRDDLGRQIDEALPG
                     YPVELAGLDEVPNAGENFYQVDSLQRAKAVAESVRNQRRANVLTRARKPQTLEELVRQ
                     REGEAVPELNIILKADVQGSLDALNKALSEIPSEQVKLHFLHAGIGAVTESDVVLAAA
                     SNALVVGFNVVADPSAQRLAETEGVDIRLYRVIYNLIDDVRNALEGLLPKERTEEKRG
                     RAEVREVFRISRVGAVAGCMVTEGPILRSHLARVIRDGQIIVPTADDLKRERHRAIAS
                     LRRFKDDVREVRAGMECGIRIENFDDIKPGDVIETYEIVETTRKL"
     gene            1176..1472
                     /locus_tag="P5316_14890"
     CDS             1176..1472
                     /locus_tag="P5316_14890"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRR86291.1"
                     /translation="MASDRMVVGTLSLRLAIFDAYSLKDKRRVVNSLKDRLKGRFNVS
                     VAEVGSLDLRQQAELGVAMVANESRFVESALDKMVDYVRQDTSAALVDYRIEVF"
     gene            1553..1975
                     /gene="rbfA"
                     /locus_tag="P5316_14895"
     CDS             1553..1975
                     /gene="rbfA"
                     /locus_tag="P5316_14895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRR86292.1"
                     /translation="MRGHRPERIATIVRAVVSDAIATKLSDPRIAPLASVTRVEVSGD
                     LEYAKVWISVLGEESIQRRTLAGLCSAAGYVQRLVGSELSIRHCPKLSFHLDTSLKKA
                     AETIRLINEAAAEARRRVGEAESSDPEDSMEASSGEQA"
     gene            1972..2850
                     /locus_tag="P5316_14900"
     CDS             1972..2850
                     /locus_tag="P5316_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86293.1"
                     /translation="MKHGGDYARRLRQLFNRLVRKFGKPTIPEPPDPLEQLLVAILGS
                     NTSHYKGFTAFKKLREQMVDLNELRVTPVSELAKIISPLVPQAEMKAQRIVDALNDIR
                     RRHDTLDLSFLKQRGRREAREYLESLEGVDRYAAASVALFSLGGHAIPVDELTLQMLR
                     KDEVVDPAADLPAVQSFLEHHIGASDAAVFSLLLDRYAASRGLRLSEAPVGEPVDRVA
                     SQAVAGPSGSASSRSAADARDHGSQGAGSRPMAGSSVVRPIRSPRRIKPSRSSPTAGE
                     RARSPASRAAKAGKRK"
     gene            2924..3826
                     /gene="hisG"
                     /locus_tag="P5316_14905"
     CDS             2924..3826
                     /gene="hisG"
                     /locus_tag="P5316_14905"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRR86294.1"
                     /translation="MLFKENDSLAQDHVLKIGIPKGSLTDSTVDLFHRAGFELYVSGR
                     DYYPSIDDPELSCVMFRAQEMSRYVEDGVIDAGLTGYDWICENGSDVREVCELIYSKA
                     TTRPARWVLAVPNESKARGPEDLDGGIVATELVNVTRRYFEKRGLNVRVEFSWGATEV
                     KARLVDGIVELTETGSSLQANNLKIVDEILASTTRFIANKQAYEDPWKREKIDALALL
                     LKGAIEARAKVGLKLNIPKASLEKLLKILPAELSPTISSLADEQYAAVEVILEAVVER
                     QLVPQLQKVGGTGIVVYPLNKVIP"
     gene            3944..7564
                     /locus_tag="P5316_14910"
     CDS             3944..7564
                     /locus_tag="P5316_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86295.1"
                     /translation="MSVTWDRRMGGLLPALVVLACMLSGCESPTRPVGGAVRLPWLPP
                     IQPPAGFDRQRAAYSLAQIPDDPPAPTVVEGKQTELPEAMRRQLSEAREQLNKGRHTE
                     AFVLLEQLLRTDAGVAEAHRLVAVAALMAGNDERAELSAGRALKLHPGDLISHYVVGR
                     LAEKRRDTAGAIRAYRTALKCKASPDEVHHLVLTHYRLGLLLDQERYYLAAAEQLGLF
                     EKGIRSLGNKVEENAELASIVRLQRGAVAIRLARAHSYLGDYSAAADALKIAVAESPK
                     DLDLRADYVRMLAHAGRLDEAMAESRRLVADSQGNREAVELLLGLHRAAGRPEAGVAA
                     MREVVAGQSDNLDLNLLYCEALVDAGRYGQAASLLKELTDRFPKSSEARWRLVSIQRL
                     RQDWSGWLLALAQMLAADPVVYEKANRELDQAPLSITRRMADEASSKGAVRQLLPAQP
                     EEGVLSAALDYLLGHICGRLDRIEDARMLFDQSAARLPGFLPAIMGAAELYMHRCRWD
                     EAIAVLKKAAELGPPSHLIERLLGQCYDGLDEMERAIEHYKAAIKINGEDVRSMMLLA
                     GLHERREESREAQRQYQAVISVDPNYMPGREAYIRSLMSAGGGFTSAVVAGRVALEFA
                     EMQRRGPRDPATIRTAVLLRFLQDRDRESYGNALRSLIQAYPEDERIREDLAASLLAF
                     RDYEAAREVLAQMVVDFPRSGQAGWMMAMTLTRLLEFDAAAAEYERILAIHPNRTSYL
                     QALAELRMTQQRFDAAIPVYERLLALQGKEGFPGVYRGRLMEAYRRLGRLDDARRRAE
                     EWLAEVDPKAKTAAPLVAIYRWFVLAADEAAKDYDAYLHRCREWLKSEPEDANTRAWL
                     IGLTTDAPVGTVGRLGGHGGLIGAGRHDEAVAQVVKWLDRSPTDARLMRLLADVFVAS
                     RRFDEAIEIQRSLAAATTKADEKLVLLYGLQTTYSRARRYDDAIATVREWMNAAQKLP
                     GDDRGLTRQELDGAVFEQRRTIGMLMAQQGRTDEAITYLTGLLEQEPDDSRRVELLRG
                     LSYIFQRQGRHNPAEQHLRQAYQLMPADVGLNNDLGYTLAEAGKDLADAERMLRLAVG
                     ESPRQAAYLDSLGWVFYKQGKFEDACRWLQLAAGQEHGEDPVIHDHLGDACWRAGEKA
                     AAADHWRQALDLYERQLADGVTERNDTLLASLRSKLAAVAADQEPAVAPLGLMQSSAP
                     TE"
     gene            7750..8499
                     /locus_tag="P5316_14915"
     CDS             7750..8499
                     /locus_tag="P5316_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRR86296.1"
                     /translation="MAGHSHWARIKHKKGVTDARRGRLWSKLARIIIVAARAGGDPRE
                     NLPLRYAIEKAREANMPKDTIENAIKKGTGELGGTSYESVRYEGYGAGGVAILCEALT
                     DNRNRTAPEIKKIFERHGGSLGSAGCVAWIFSSKGQVTVPVGAADEDKIAEIALEAGA
                     DDYRQSDDVWEITCEPAAYEPLRAALAAAGIAPQSSELSMIPSNTVTVDAENGRKVLS
                     LIEALEEHDDIQNVYSNFELPDDVMASLGQG"
     gene            complement(8759..9214)
                     /locus_tag="P5316_14920"
     CDS             complement(8759..9214)
                     /locus_tag="P5316_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86297.1"
                     /translation="MTRIMRYALAALLIGTVCGCRYSLPPGFVKVDPIWDTRVRAVSA
                     EGAAITLRVETNPENGDLAFWEKAIMTRLVEFRGYRLADRREIKHGDETPGVQMTFDY
                     QRDGIDYTYVVNVMVKGRKVYCIEIAGEKTDIAPQLTAISDAVRKWPLS"
     gene            complement(9258..10028)
                     /locus_tag="P5316_14925"
     CDS             complement(9258..10028)
                     /locus_tag="P5316_14925"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRR86298.1"
                     /translation="MRSRPQILALIGFTWLTTIPLLLGCAYYGKMQAMRAAKELAEPP
                     ATADDAPTAGVAMERPLARLAADKGVDSAAPAAQRMLIYTGRLGMAVADIEASVQATQ
                     RLADEVGGYMLAMSANSITIRVPAKQFFPILERLKALGQTLSKNVTAQDVTDEYTDLQ
                     LRLKNAEALRDRLVAILEKAANVKDTLEVERELNRVREEIERIKGRIAQLDKLIAFST
                     IEVSFTQAQDQPAKIRRPETPFPWLDQLGVETVLGIRG"
     gene            complement(10248..10811)
                     /locus_tag="P5316_14930"
     CDS             complement(10248..10811)
                     /locus_tag="P5316_14930"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRR86299.1"
                     /translation="MTQPSDKPYVSRGGEKLAAALSAFGIEPRGWICADLGSNVGGFV
                     DCLLRRGAGRVYAVDTGYGVLAYELRTDPRVVVLERTNAMHLALPEHVDLVTIDVAWT
                     PQKRILPAALRMLKPNGRIISLVKPHYETDRRHLRHGVLDPTESKAAFESVCAEIEAE
                     GMAVARWIESPIKGQKGNVEYLVLLKR"
     gene            complement(10963..11235)
                     /gene="rpsT"
                     /locus_tag="P5316_14935"
     CDS             complement(10963..11235)
                     /gene="rpsT"
                     /locus_tag="P5316_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRR86300.1"
                     /translation="MAHSLSAKKRIRQNAKRRARNRGRKRALKVDVREFEAALAGGDK
                     AKAAEALKAAVKSISKTAAKGTLHKNAASRRISRLQKKYNALAAKA"
     gene            complement(11261..>12335)
                     /locus_tag="P5316_14940"
     CDS             complement(11261..>12335)
                     /locus_tag="P5316_14940"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86301.1"
                     /translation="QARFDKTAMWIGEILGDAVVHEPKPNPARFITLRQTYEMLGDLN
                     AETDPPLAIEAMDRAVDALTKLRALKPHDGELAVALRDVAGKLTILKGRYSSAESFTD
                     SIMDKDSQRDLHDKDRLVQSDDRLDNLIAQARQRYEADPTNGTLIHELVDLLCRREQD
                     QEEAQAMRILLKAYEQTDEYRYKLRAHDIYMKQLRRKARLLEEAGDPQAHAARAKQLK
                     VELAVYKERVEQYPTELNYRFEYGRRLFEAGRYDDAIPVLQEARNEPKNRFRCSLYIG
                     RCFFEKGFYKQAANVFREAIASYEIPNDDLGKRLHYWLGRALEADGNAPEALKIYGQL
                     IEWDYNYRKGDVRKRIENLHSKS"
BASE COUNT         2253 a         3396 c         4187 g         2499 t
ORIGIN      
        1 accgagtggg gcggggaaat cgacgttgtg aagacatccg ccgtcaccgg ccagggcgtc
       61 ccggagttga tcgagcatct gggaatgctc acggagttgt tgaacctgcg ggcggacccc
      121 gacgtgccgg ccaccggcac ggtcatcgaa gcccagatga ctcagggagt cggtcccctt
      181 gctcgcgtat tgattcggga gggcacgctc aggccggggg ctttcatcgt gtgtggcagc
      241 gccgcggggc gcgtccgggt gatgcgggac gatctcggca gacagattga cgaggccctg
      301 ccgggctatc ccgtcgagct ggccggactg gatgaagtgc ccaacgcagg tgaaaacttc
      361 tatcaggtgg attcattgca gcgggccaag gcggtggccg agtcggtccg gaatcagcga
      421 cgggcgaatg ttctgacccg ggcgcgcaag ccgcagacgc tggaagagct ggtgcggcag
      481 cgtgagggcg aggcggttcc cgagttgaac atcatcctga aggccgatgt tcagggttcc
      541 ctggatgccc tgaacaaggc cttgagcgag attccgagcg agcaggtgaa gctccatttc
      601 ctgcacgccg gcatcggggc ggtgacggaa agcgacgtcg tgctggctgc agcgagcaat
      661 gcgttggtag tcggcttcaa cgtggtggcg gacccgagcg ctcaacggct cgccgagacc
      721 gagggcgtgg acatccggtt gtaccgggtg atctacaacc tgattgacga cgttcggaac
      781 gcgttggagg gcctgttgcc gaaggagagg accgaagaga agcgcggccg ggccgaggtg
      841 cgcgaggttt tccgcatttc ccgcgtcggg gccgttgccg gctgcatggt gaccgagggg
      901 ccgatcctgc ggtcacacct ggcgcgggtg atccgcgacg ggcagattat tgttccgacg
      961 gcggacgatc tgaagcggga acggcacagg gcgatcgcat ccctgcggcg gttcaaagat
     1021 gacgttcgag aggttcgcgc agggatggag tgcgggatcc ggatcgaaaa cttcgatgac
     1081 atcaaacccg gggacgtcat cgagacgtat gaaatcgttg agacgacccg caaattgtag
     1141 gcgggcggaa ccggcggacg tgtgggcgga tcagcatggc ctccgatcgg atggtggtgg
     1201 ggactctcag cctgaggctg gcgatttttg acgcttattc gctgaaggac aagcggcggg
     1261 tggtcaacag cctgaaagac cggttgaagg ggcggttcaa cgtttcggtc gccgaggtcg
     1321 ggtcgttgga tctccggcaa caggccgagc tgggcgtggc gatggtggcg aacgagagcc
     1381 ggtttgtgga gagcgccctg gataagatgg ttgactatgt tcgccaggac acatccgcgg
     1441 cgctggtcga ctatcggatc gaagtgttct agtgcgacgg cggggtccgg atgcgggtac
     1501 aagaattccg gaccgtgggg tcgggccgtt gtcgggcggg gcggtgatgg gcatgagagg
     1561 tcacaggccg gaacggattg cgacgatcgt gagagcggtg gtgagcgacg cgatcgcgac
     1621 caaactgagc gatccgcgga tcgcgccgct ggcgagcgtg acccgcgtgg aggtgagcgg
     1681 cgacctggag tacgcgaaag tctggatcag cgtgctaggg gaggagtcca tccagcgacg
     1741 tacgctggcg ggtctttgtt cggcggcggg gtacgtgcag cggctggtgg gcagcgagtt
     1801 gtcaatccgg cactgtccga agttgagttt tcatcttgac acctcgctta agaaggcggc
     1861 ggagacgatc cggctgatca atgaagcggc agcggaggca agacgacggg tcggcgaggc
     1921 ggaatcgtcc gatcccgaag actccatgga ggcttcctcg ggagaacagg catgaagcat
     1981 ggcggtgatt atgccaggcg cctcaggcaa ctgttcaacc ggctggtgcg caagttcggc
     2041 aaaccgacca ttccggaacc tccggacccg ctcgagcaac tgctggtggc gattctcggg
     2101 agcaacacgt ctcactacaa ggggttcacg gcgttcaaga aactgcgcga acagatggtc
     2161 gatctgaacg agttgcgtgt cacgcccgtc agcgagttgg cgaagatcat cagtcctctg
     2221 gtcccgcagg cggagatgaa ggctcagcgt attgtggacg ctctgaatga cattcgtcgc
     2281 aggcatgaca cgcttgacct ttccttcctg aagcagcgag gtcggcgcga agctcgcgaa
     2341 tatctggagt ccctggaagg agtggaccgt tacgccgccg ccagcgtggc gttgttcagc
     2401 ctcggcggtc atgcgatacc ggtggacgag ctgaccctcc aaatgttgcg gaaggacgag
     2461 gtggtggatc ccgctgcgga cctgccggcg gttcagagtt tcctggagca ccatatcggc
     2521 gcgtcggatg ccgcggtttt ttcgttgctg ctggatcgtt acgcggcaag tcgcggtctg
     2581 cggctgtcgg aggcgccggt gggagagccg gtcgaccggg ttgcatctca agcggttgcg
     2641 gggccaagcg ggtccgcttc cagtagatcg gctgcggatg cgcgagatca cggttcgcaa
     2701 ggagccggca gcaggccgat ggccggttca tcggtcgtcc ggcctatacg gtcacccagg
     2761 agaatcaagc cttcccgctc gtcgccgacg gccggcgaac gcgcccggtc gccggcaagt
     2821 cgagcggcca aggccggcaa gcggaaatga ggagccggcc ctcaagaccg ggatcgagcc
     2881 ggtccttgct tgagtcgagc acgaccgccg accgccggct gcaatgcttt tcaaggagaa
     2941 tgactcattg gcacaggatc acgttctcaa gatcggtatt cccaaaggca gtttgacgga
     3001 ctcgacggtc gatctttttc atcgtgcggg ttttgagctt tatgtttcgg gtcgcgacta
     3061 ctacccttcg attgacgatc cggagctctc gtgcgtgatg ttccgggccc aggagatgag
     3121 ccgttacgtc gaggacggcg tgattgacgc cggcctgacg ggctatgact ggatctgcga
     3181 aaacggcagc gacgtgcgcg aggtgtgcga actgatttat tcgaaagcca cgacccggcc
     3241 tgcccgttgg gtgctggcgg tgccgaacga atccaaagct cgcgggcccg aagatctgga
     3301 cgggggtatt gtggcgaccg agctggtgaa cgtcacgagg cgatacttcg agaaaagggg
     3361 gctcaacgtt cgcgtcgagt tcagttgggg ggcgacggag gtcaaagcgc gtctcgttga
     3421 tggtattgtc gagctgaccg agaccggatc atcgttgcag gccaacaacc tcaagattgt
     3481 ggacgagatt cttgcttcga cgacacgatt cattgccaat aagcaggctt acgaagatcc
     3541 ctggaagcgc gagaagatcg acgccctggc gctcttgctg aaaggggcga tcgaggcgcg
     3601 ggccaaagtg ggattgaaac tcaatattcc gaaggcgagc ctggaaaagt tgctgaagat
     3661 cctgcccgcg gagttgtcgc cgaccatttc gtctctggct gacgagcagt atgcggcggt
     3721 ggaggtaatc cttgaggcgg ttgtcgagag gcaattggtg ccgcaactgc agaaggtcgg
     3781 cggaacgggg attgtcgttt atccactgaa caaggtgatt ccgtagccgg cgatcggttg
     3841 cccgagtgaa ggcatgtcct ggtgcggcgg cgcgtgcgat gaaaggcgga acggccgccg
     3901 aagatcgcga tggcggcggt gtgatgaagt catgagacgc gtaatgtcgg taacgtggga
     3961 ccggcggatg ggcggattgc ttccggccct tgtggttctg gcatgcatgt tgagcggctg
     4021 cgaaagcccg acgcgacctg tcgggggtgc ggtcagattg ccgtggttgc cgccgataca
     4081 gcctcccgcg ggttttgatc gccagcgggc tgcgtattcc ttggcacaga taccggatga
     4141 tccgcccgct ccgacggtcg tggagggaaa gcagaccgag ttgcccgaag caatgcggcg
     4201 tcagttatct gaagcccgtg agcagctcaa taaagggcgg cacaccgaag ccttcgtgtt
     4261 gcttgaacag ttgcttcgaa ctgatgcggg tgtggctgag gctcatcgac tggttgccgt
     4321 cgccgccctg atggccggca atgatgagcg cgcggagctg tcggccggcc gtgcgctgaa
     4381 gctgcatcca ggcgatctca tcagtcatta cgtcgtcggt cgcctggcgg agaagcgacg
     4441 tgatacggcg ggggccattc gtgcctaccg gactgcgctg aagtgcaaag catcgccgga
     4501 cgaggtccac cacctcgttc tgacccacta ccgacttggt ctcctgctgg atcaggagag
     4561 gtactatttg gctgcggccg agcaactggg cctttttgag aagggcattc gctcgctggg
     4621 gaacaaggtc gaggagaacg ccgaactcgc ttccattgtt cggctgcagc gaggtgcggt
     4681 cgccattcgc ctggctcgag cgcacagtta tctgggagac tattcggcgg cggcggatgc
     4741 tttgaagatc gcggtggcgg aatcgccgaa ggatcttgat ttgcgcgccg actacgtgcg
     4801 gatgctggcg cacgccggtc gtctggacga agccatggcg gaatctcgcc gacttgttgc
     4861 ggacagccag gggaaccggg aggcggttga actgctgctg ggcctgcaca gggcagcagg
     4921 gcggccggag gcgggcgttg ccgccatgcg ggaggttgtg gccgggcagt cggacaactt
     4981 ggacttgaat ctgctctact gcgaggcgct ggtggatgcc gggcgatatg gccaggcggc
     5041 ttcgttgctc aaggagctga ccgaccggtt cccgaagtcg tcggaggcga gatggagact
     5101 ggtttccatt cagaggcttc ggcaggattg gtcgggttgg cttcttgctc tcgcccagat
     5161 gctggcggcg gatccggttg tgtacgaaaa ggcgaaccgg gagcttgatc aagcaccttt
     5221 gtcgatcacc aggcgcatgg ccgacgaggc ctcttccaag ggggcagtgc gacaactctt
     5281 gcccgcacag cctgaagagg gcgtgttgtc ggcggcgttg gattatcttc tgggccacat
     5341 ttgcggtcgt ctcgaccgca tcgaagacgc ccgcatgctt tttgaccaat cggcggcgcg
     5401 cctgccgggt tttttgcctg cgatcatggg tgcggccgag ttgtacatgc accgctgtcg
     5461 ttgggatgag gcgatcgccg ttctgaagaa ggccgcggaa ttgggtccac catcacatct
     5521 tatcgagcgc ttgctgggcc agtgttacga cggcctcgac gagatggagc gggcgatcga
     5581 acactacaag gcggccatca agatcaacgg cgaggacgtc cgcagcatga tgctgttggc
     5641 cgggttgcat gagcgccggg aggaaagccg tgaagcccag cggcagtatc aggcggtgat
     5701 cagcgtcgat ccgaactaca tgcccggtcg cgaggcctac attcgcagcc tgatgagcgc
     5761 cggcggcgga ttcacctccg ccgttgtcgc ggggcgggtg gcgctggagt tcgccgaaat
     5821 gcagaggcga ggtccgcgtg atcctgcgac gattcggacc gccgtcttgt tgcgatttct
     5881 tcaggatcgc gatcgcgagt cttatggtaa tgcgcttcgg tcgttgattc aggcatatcc
     5941 tgaggacgaa cggattcgcg aggatctggc ggccagcctg ttggcctttc gggactacga
     6001 agccgcccgc gaggttctgg ctcaaatggt tgtcgatttt ccccgatccg ggcaagcagg
     6061 ttggatgatg gcgatgaccc tgacccggtt gctggagttc gacgctgctg ccgcggagta
     6121 cgagcggatt ctcgccattc atcccaatcg tacgagctat cttcaggctc tggcggagct
     6181 gcgaatgacc cagcagcgat ttgacgctgc cattccggtg tatgagcgac ttcttgcact
     6241 gcagggcaag gaaggttttc cgggcgttta tcgcggccga ctgatggagg cttatcgccg
     6301 actcggtcgt cttgacgatg cgcgccggcg tgcggaggag tggcttgccg aagtggaccc
     6361 caaggccaag accgccgcgc cgctggtcgc tatctatcgc tggtttgtgc tggcggcgga
     6421 tgaggcggcg aaggattacg acgcttacct gcatcgttgt cgggagtggc tgaaatccga
     6481 gcctgaggat gccaacactc gggcctggct gatcggcctg acgacggatg cacccgtagg
     6541 cacggtcggc aggttgggag gtcacggcgg gctgattggt gccgggcgtc acgatgaggc
     6601 cgtcgcgcag gtggtaaagt ggcttgacag atcgcccacg gacgcgcggt tgatgcgcct
     6661 gctggccgac gtcttcgtgg cttcacggcg ttttgatgag gcgatagaga ttcagcgcag
     6721 cctggcagcc gccacgacga aggctgatga aaagctggtc ttgctttacg gtcttcagac
     6781 cacgtacagt cgagcccgcc gctacgatga tgccattgcg accgttcgcg agtggatgaa
     6841 tgccgcccag aagttgccgg gggacgaccg cggtctgacc cgccaggagc tggatggggc
     6901 ggttttcgag cagcggcgca cgatcggcat gctgatggct cagcaaggcc ggacggacga
     6961 agcgatcacc tacctgacgg gcctgttgga gcaggagccg gacgattcgc gacgggttga
     7021 gctgttgcgg ggcctttctt acatctttca gcgtcaggga cggcacaacc cggcggagca
     7081 acaccttcgt caagcctatc agctcatgcc ggccgacgtg ggtttgaata acgatctggg
     7141 gtacacgctg gccgaggccg gcaaggactt ggccgatgcc gaacggatgt tgcgactggc
     7201 agtgggcgag agtcctcgcc aagcggccta tctggacagt ctgggttggg ttttctacaa
     7261 acagggcaag tttgaagatg cgtgccgctg gctgcaattg gcggccggcc aggagcacgg
     7321 cgaggatccg gtgattcatg atcacctcgg cgatgcgtgc tggcgtgcgg gagagaaggc
     7381 cgcggcggct gatcattggc gacaggcatt ggatttgtac gagcggcaac tggccgacgg
     7441 cgtcacggag cgaaacgata cgttgcttgc cagcctcagg agcaaactgg ccgctgtggc
     7501 tgcggatcag gagcctgcgg tggctcctct cgggctcatg caatcctcgg caccgaccga
     7561 atagcctttt tggcgacgtc acccttgagt gatggatgcc tcatggggta aagtcgaggg
     7621 agcaggttga gcaggtattg gggtattgag tccgagtctt gcgagggcct ggccggctcg
     7681 ggcggagctg gacggcggcg gatgaaggtt ttctcgcgga tcggtgcctg agattagagg
     7741 agacgttaga tggctggtca ttctcactgg gcgcgaatca aacataagaa aggtgtaacc
     7801 gacgcccgaa gagggcgtct gtggagcaaa ctcgcccgga tcatcatcgt ggctgctcgt
     7861 gccggaggcg accctcggga aaacctgccc ctgcgttatg cgattgaaaa agcgcgtgaa
     7921 gccaacatgc ccaaagacac cattgagaac gccatcaaga agggcaccgg cgaactggga
     7981 ggaacgagtt acgagagtgt tcgctacgaa ggatacggag ccggtggtgt agccattctc
     8041 tgtgaagcct tgacggataa ccgcaaccgg actgcgcccg agattaagaa gatcttcgag
     8101 cggcacggcg gttcgctggg cagcgccgga tgcgtggcgt ggatattcag cagcaagggg
     8161 caagtgaccg ttccggtcgg tgcggccgac gaggataaga tcgctgagat cgccttggag
     8221 gccggagctg acgactaccg gcaatccgac gatgtctggg aaatcacttg cgagccggcg
     8281 gcatatgagc cgttgcgcgc ggcgttggct gcggccggaa tcgctccaca atcctccgag
     8341 ttgtcaatga tcccgtcgaa cacggtcacc gtcgatgccg agaacggcag gaaggtcctc
     8401 agccttatcg aggcgttgga ggaacacgac gacatccaga acgtttactc gaatttcgag
     8461 ctgcccgacg acgtgatggc ctcgctgggg cagggatgac ggcggtatcg aattgcgcat
     8521 cctgtcgagg gtcgcctggg cggctcgctt ctcgcgaggg cggggcacag catggcgatg
     8581 ctgacaaccg ttttccggca tggcccagca tggagcaaat ccgcgacacg gcgaagcgac
     8641 ctgtagaaac aggcatttta atacgtatca aataataaaa gcccccttaa tacaccttcg
     8701 ttgtactttg ccgcggcgga tggagtaccc gaccgcggca ggccgatccg ggcgacttct
     8761 atgacagagg ccacttgcgg acggcgtcgg agatggcggt caactgcgga gcgatgtctg
     8821 ttttttctcc cgcgatctcg atacagtaga ccttgcgacc cttgaccatg acgttgacaa
     8881 cgtatgtgta gtcgatgccg tcgcgctggt agtcgaaggt catttggacg ccgggtgtct
     8941 cgtcgccgtg cttgatctcc cggcgatccg cgagcctgta gcctcggaat tccacgaggc
     9001 gcgtcatgat ggctttttcc cagaaggcca ggtcgccgtt ctccgggttg gtctcgaccc
     9061 gcagggtgat ggcagcccct tcagccgaca ccgctcggac gcgggtgtcc cagatcgggt
     9121 cgactttgac gaaacccggc ggcaacgagt accggcagcc gcacactgtt ccgatcaaca
     9181 gagctgcaag tgcatatcgc atgattcgcg tcatggtcgt atctcccgtt cgttttttcc
     9241 caaaccacat gtccgtatca acctcgaatt cccagtacgg tctccacgcc gagttggtcc
     9301 agccagggaa acggcgtctc cggccttcgg atcttggccg gctggtcctg tgcctgggta
     9361 aacgacacct cgatggtgct gaaggcgatc agtttgtcga gctgggcgat tcgccccttg
     9421 atacgctcga tttcctcgcg tacgcgattc agttcgcgtt cgacctccag cgtgtccttg
     9481 acgtttgctg ccttctcgag gatcgccacg agccgatctc gcagggcctc ggcgttcttg
     9541 agccgcagtt gcagatcggt gtactcgtcg gtgacatcct gagcggtgac gttcttcgag
     9601 agggtctggc ccagggcctt gagcctctcg aggatgggga agaactgctt ggccggcacg
     9661 cgaatggtga ttgagttggc gctcatggcc agcatgtacc caccgacctc gtcggccagt
     9721 cgctgggtcg cctggacgga agcttcgata tcggctaccg ccatgcccag acggccggtg
     9781 tagatgagca tccgctgggc ggccggcgca gccgagtcca cgcccttgtc ggcggccagg
     9841 cgggccaagg gccgctccat cgcgacacca gcggtcgggg catcgtccgc agtcgcaggc
     9901 ggctccgcaa gctccttggc cgcccgcatt gcctgcattt tgccgtagta cgcgcagccc
     9961 aataaaaggg gaatcgtagt taaccatgtg aatccgatca gagcaaggat ctgtggtcgt
    10021 gaccgcataa ggcacctccc atgagtaacc gacaaggccg gcccacccac agggccggtc
    10081 tgcatcctac ccccgcccct gcccaagcga cagtgcatct ttgtcatcca tgtagccccc
    10141 gacgacatgg tttggttccc tgccttttcg tttcttgtgg gcattgccgg ccggtaaatg
    10201 gctgggggcg tcccttgtgg cggcaagggg aggggatgac ggtcatctta tcgtttgagc
    10261 aggaccaggt actcgacgtt tcctttttgc cccttgatcg ggctttcgat ccatcgggcg
    10321 acggccatac cctcggcctc gatctcggcg cagacactct caaatgcggc cttggattcg
    10381 gtcgggtcga gtacgccgtg ccgcaggtgc ctgcggtcgg tctcgtaatg cggtttgacc
    10441 agggagatga ttctgccgtt gggtttgagc attctgaggg ccgccggcag tatgcgtttc
    10501 tggggggtcc aggcgacgtc gatggtcacc agatcgacgt gttcgggcag cgcgaggtgc
    10561 atggcgttgg tccgctccaa gaccacgacg cgagggtctg ttctcagctc ataggcgagc
    10621 acgccgtagc cggtgtcgac ggcatagact cggcctgcac cgcggcgcag caggcaatcc
    10681 acgaagcccc cgacattgct gcccaggtcg gcacagatcc agccacgagg ctcgatgccg
    10741 aacgcgctga gggcggcagc gagtttttct ccgcctcggc tgacataagg tttgtcgctg
    10801 ggctgagtca tagtccggct caggcagatt gacgagcctt tcgaggtttc ggggggttgg
    10861 gtgtcagttg cgtgcctcgg tttcccagga tgaaaagaag cgcgggcgac cgatcgatcg
    10921 cccgcggatg atcttgaccg atccggccca acgacggaga ccttacgcct tggcggccaa
    10981 ggcgttatac ttcttctgaa gtcgtgaaat ccgccgactg gcggcattct tgtggagcgt
    11041 tcccttggcc gccgtcttgc tgatcgactt cacggcggct ttcagcgcct cggcggcctt
    11101 ggccttgtcg ccaccggcca gcgccgcctc gaattcccgc acgtcgacct tgagagcccg
    11161 cttgcgaccc cggttcctgg cccgtctctt ggcgttttgt cgaatccgct tcttcgcgga
    11221 aagtgaatga gccactagtt gaatctccta ccttgtcgcg tcaactcttg ctgtggaggt
    11281 tttctatgcg tttcctaaca tctcccttac ggtagttata atcccattct atgagctgac
    11341 cgtagatttt caaggcctcc ggggcgttgc cgtcagcttc gagagcgcgg cccagccagt
    11401 agtgaagtct cttgccgaga tcgtcgttgg ggatttcgta gctcgcaatg gcttcccgga
    11461 agacgttagc cgcctgcttg taaaagcctt tctcgaagaa acagcggccg atgtacaggc
    11521 tgcatcggaa ccgattcttg ggttcgtttc gggcctcctg cagaacagga atggcatcgt
    11581 cgtagcgacc ggcctcgaag agtctgcggc cgtattcaaa gcggtagttc agctcggtcg
    11641 ggtactgctc aacgcgttcc ttatagacgg ccagctcgac tttcagttgc ttggctcttg
    11701 cggcgtgcgc ttgggggtct ccggcttcct cgagcaggcg agcttttcgc ctgagttgtt
    11761 tcatatagat gtcatgggcc cgcagtttgt accgatactc gtcggtttgc tcataggctt
    11821 tgagcaggat tctcatggcc tgggcttcct cttggtcctg ctcgcggcgg caaagcaaat
    11881 cgacaagctc atggatcaga gtcccgttgg tgggatcggc ttcgtagcgt tgtcgcgctt
    11941 gagcgatcag gttgtcaagg cggtcgtcgg actgaacgag ccggtctttg tcgtgcaggt
    12001 ctcgctgcga atccttgtcc atgatggaat cggtgaacga ctcggccgac gaatagcgcc
    12061 ctttcaggat agtcagtttt ccggcgacat cgcggagggc cacggccaat tcaccgtcgt
    12121 ggggtttgag ggcccggagt ttggtaaggg catccacggc gcggtccatg gcctcaatgg
    12181 ccaggggcgg gtcggtttcg gcgttcaggt cgccaagcat ttcataagtt tggcgcaagg
    12241 tgatgaagcg agcggggttg ggcttgggtt catgaaccac cgcatcgccc aggatctcgc
    12301 cgatccacat cgcggtcttg tcgaaacggg cttgg
//
LOCUS       DAOTPW010000172        12326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_4928_length_12326_cov_5.490506, whole genome shotgun sequence.
ACCESSION   DAOTPW010000172 DAOTPW010000000
VERSION     DAOTPW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12326
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4928_length_12326_cov_5.490506"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1132
                     /locus_tag="P5316_14945"
     CDS             425..1132
                     /locus_tag="P5316_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86302.1"
                     /translation="MLPVGRVAFAGLVTVLAVAMMADATEVSLYSIDGRQTGNANRTG
                     SRRLQEFNPSLSMPNSIGGPGQEYFDRVGMRFGLDAGERIWGELDLYFWLNDVYQTYG
                     QDPACRKQIDVTNTSDQYADVWLELDLNVLSDPPFPVPPHPWPDPLGPVPAKGRYLLR
                     FDCRGMSGGNSRYDIYWSTNGDDGDENQAWQWDSGASAPWAYPGGAWDVRVNAMPEPA
                     AALLLVLGGVVGLRRRR"
     gene            1618..1878
                     /gene="rpsP"
                     /locus_tag="P5316_14950"
     CDS             1618..1878
                     /gene="rpsP"
                     /locus_tag="P5316_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRR86303.1"
                     /translation="MVRLRLKRTGRRHRPTYRLAAVDSRSPRDGMVIEELGVYEPANQ
                     NPDLRCKLKQERVEYWLKVGARPSDTARDLLKRLGYQVSVRK"
     gene            1888..2598
                     /gene="trmD"
                     /locus_tag="P5316_14955"
     CDS             1888..2598
                     /gene="trmD"
                     /locus_tag="P5316_14955"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRR86304.1"
                     /translation="MRIDVLTLFPGVMEPFFASSIIARARAAGIVSIWCTNPRDFTTD
                     RHRSVDDRPFGGGPGMVMMCGPLFDAVEKLEKDDPTPAVRILLTPQGERFDQAIAREL
                     AREKRLILICGHYEGFDERIRIGLKPREISIGDYVLSGGEVAAMAVVDTVVRLLPGAL
                     GDEASAEHESFSGRVLEYPQYTRPREFRGMQVPEVLLSGDHAKIAEWRAEQAAKRTAE
                     RRPDLLAPANSKEDDGRG"
     gene            2618..3004
                     /gene="rplS"
                     /locus_tag="P5316_14960"
     CDS             2618..3004
                     /gene="rplS"
                     /locus_tag="P5316_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRR86305.1"
                     /translation="MINALIEKVESAAKKKQLPKFSIGDTVSVQVRIVEGGKERIQPF
                     IGVVIGRRGRGMGESFTVRRIVNNEGVERIFPLHSPKVAGVEIIRSGHIRRAKLYYLR
                     DRVGKARRLRDRKRDARQAVDAAVQQ"
     gene            3107..3475
                     /locus_tag="P5316_14965"
     CDS             3107..3475
                     /locus_tag="P5316_14965"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRR86306.1"
                     /translation="MDDRARLGRAGEKESERYLRRQGHKIVCRNYRCPAGEIDLISLD
                     GKTIVFTEVKTRSDSERANPEDAVNPAKRHRLVASAKCFIQQTRSQGRAYRFDILALT
                     ANAKGGLDIEHFKGAFSCKC"
     gene            3480..4280
                     /locus_tag="P5316_14970"
     CDS             3480..4280
                     /locus_tag="P5316_14970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRR86307.1"
                     /translation="MHYELFDTHCHLTIDDLYPRREQVVADAAAAGVTRMITVACVPD
                     EWETASAMRSDFPGVWVAASVHPHEAGKVGADDLDRLAGVWRRPGVVAAGEMGLDYHY
                     DFSPRQVQQLVFERQLELAWDTGLPLVIHCREAHDDVVRLLEKHGYRGKPVVFHCFGG
                     GPEEAADLRSRGWRVSFSGILTFKKARQAQAACAETPIDQILFETDAPYLSPEPVRNR
                     RPNEPAYVAYTVRFAAQLRGETFETLAAASTGNAVKLFAPAEHGDHKP"
     gene            complement(5057..5446)
                     /locus_tag="P5316_14975"
     CDS             complement(5057..5446)
                     /locus_tag="P5316_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRR86308.1"
                     /translation="MALRIAIDVNRYVDFARGIDEAVACVRRAGQIYLPFVVLAELRA
                     GFRAGQRAKANEAVLSRFLQSDRVTVLLPDEQTTHVYAEVFTQLRVAGTPIPTNDLWI
                     AALVIQHDLVLFSRDRHFDVLPQIARI"
     gene            complement(5437..5694)
                     /locus_tag="P5316_14980"
     CDS             complement(5437..5694)
                     /locus_tag="P5316_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86309.1"
                     /translation="MKRKNRQYTLRDIPPALDRALRERARQEGKSLNQVAREALKRGV
                     GLPEENQLADDLDCLAGTWQDDPAFDEAIRQQDTVDPETWR"
     gene            complement(5764..7335)
                     /gene="dacB"
                     /locus_tag="P5316_14985"
     CDS             complement(5764..7335)
                     /gene="dacB"
                     /locus_tag="P5316_14985"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRR86310.1"
                     /translation="MRTCHPYRFGLAGLLLILTGCSSPRIAHDPKLAARLDPVLDRYA
                     DKGVVIHARVLELPSRCEIYARRIDKPCTPASNFKLLTSAAGLDMFGADYKVKTYLAM
                     DGDDLWLIGTGDPACGDPRLAKAAGRTPVSMLQDWVTAIEQRGIRRIKGDIVYDDSAF
                     EESPKVHPTWPKHWLLHWYAAPTAGLNFNDNSVDITIYPTQPGKPPRYEVMPPAQCVK
                     VINECMTADKNAPTIAKLKEGNIYKIGGTCSEMTELKSKPVDDPGAFFADALRTQLAA
                     RGITVDGRIRKADKPLGGAIPPPCDKIIAVHETKMSDILSRINKNSQNLFAECLCKMT
                     GQAYEAKHGRRVPGSWENGGRALRAFLRKNGIDDRRLVPIDGSGLSPQNRLTTRMLTD
                     LLAVMYARPDREDYMASLTICGVDGSLKDRMTDLKGRVLGKTGYIGGVSSTSGYIKTN
                     AGKWLAFSFVYNEIPERSDDDSDVKPFTKLQDEACDILASWPNISATPTSNCADPAET
                     ATSTAQPDVVVPNAR"
     gene            complement(7355..7894)
                     /locus_tag="P5316_14990"
     CDS             complement(7355..7894)
                     /locus_tag="P5316_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRR86311.1"
                     /translation="MMTFKLILNQISMFTIPVIFLLIIGYAMIKRAKVYESFVTGGKE
                     GFDVFIRILPYLVAIFVAIGTFRVSGGLEFLSECLAPLTLKIGMPPEVLPVALMRPLS
                     GSGSLGLAAEVIQAKPDSFESQLASTVFGSTDTTFYVIAVYFGSVGIRKIRHAMIAGL
                     LADAAGVLMAIWICSMIFK"
     gene            complement(7891..8895)
                     /locus_tag="P5316_14995"
     CDS             complement(7891..8895)
                     /locus_tag="P5316_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRR86312.1"
                     /translation="MRILGGIIRYLLNINTVWGLMILAAFGLCVAQHYLPTTTVIPAD
                     AVRHGLNMLTVRIKDSGDRAASFDFRLWLGPEGLDLPADAKAKDQGRPWLISARQVQG
                     GHLLKWDSDDSGKYEVVVNDRSVGRGSLVTLQSMTDAAFDYAQRGFEICLGLVAAMVL
                     FLGLMKVGEDAGIVQLVAHVFHPIIRLLFPQVPRDHPANGAILMNMTTTILGLGNAAT
                     PFGLKAMEQLQELNPHKGVASDSQVMLLAYNTAGFALLPTTLLALRKSAGCSDPFEII
                     GTCMIAGATSTIVAILGARLLGRLPMFSLNAALAEAQREGIEPQTDAAVAKSGKEQPS
                     "
     gene            complement(9092..10564)
                     /locus_tag="P5316_15000"
     CDS             complement(9092..10564)
                     /locus_tag="P5316_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRR86313.1"
                     /translation="MKSRLTKGGPMPTTVKIDPITRIEGHMAVELTVDTVNGVQQVVD
                     ARSSGTMFRGFETLLIGRDPRDATPYTQRICGVCPIGHGMASAMALEDACGIKPPHNG
                     RILRNLVLGTDFLHSHILHFYHLAALDYINTTGILDKPPWSPGYTAADMIDGATARTL
                     VEHYITALAMRRKAHQMGAIFGGRMPCAASFVVGGATDVVTNDKISAFRSLLAEVRVF
                     VESALVPDARTLGTLFPAYFQIGTGCGNLLACGGFDLDNSGSARLLPSGRYTNGELHS
                     FAESDITESVKHSWYTPDCGTLNPASGNTAPDADKPGAYSWIKSPRYQNIVHELGPLA
                     RMWISGRYRRGISVMDRLIARTLETKQVADAMDVWLSQLIPGETACVQKPVPASAGGV
                     GLTEAPRGALGHWIRIEDRKIARYQVITPTAWNASPRDDANQPGPIEQALIGTPIADT
                     SRPIEALRVIHSFDPCLACSVHLVRPKQGATQAGLRAVAL"
     gene            complement(10561..11469)
                     /locus_tag="P5316_15005"
     CDS             complement(10561..11469)
                     /locus_tag="P5316_15005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRR86314.1"
                     /translation="MSISRRDLLKASAAVLAAPGLAGRAVAQLQQVLAGKSHPPVIWL
                     QGQGCSGCSTSLLNSIYYTTADDLLQNVIDLEYHPTLSAAAGELTVSAAEKRRGLGGY
                     ILVIEGAIPTGAAGRFCTVWEGKTMQQALVDFAPGARFILAVGSCAAFGGLVSANTNP
                     TEAKSVRGILGDDARIINIPGCPSHPDWIVGTIAHLLKHNQAPPLDANRRPMVYFENR
                     IHDYCERRCKNGDECMAGSLGSDGCLINLGCRGESTYADCYLRKWNTGRPGRFGVNWC
                     IGSGGPCLGCVNPGFPGMSSFFDSGE"
     gene            complement(11667..>12326)
                     /locus_tag="P5316_15010"
     CDS             complement(11667..>12326)
                     /locus_tag="P5316_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRR86315.1"
                     /translation="ELRVLAHFCGDQALRTAFIEDRDIHQFVAAQVFGVPLDQVTRQQ
                     RSRAKAVNFGIIYGQGAYGLSRTTGMSVTEAQSFINMYFMRYPGIRMFIDKTVAETRK
                     RGYVETILGRRRQVPEINSRNKGMRSLGERVAVNTVIQGSAADLIKRAMIGIHQRIRS
                     EQRPSRMLIQVHDELVFDVPRSAVEAEAEFIRTEMCTALPLKVPIKVDINWGDNWLEG
                     K"
BASE COUNT         2257 a         3473 c         4203 g         2393 t
ORIGIN      
        1 ttggaggcgc atgcccacgc aaagccgtgg gcatggcacc cggcgcactg cgatcaagaa
       61 acatgggcgc aagccgcaca ggtatctggc cgcagggttc taaaacgagg caacgattcg
      121 agttagctgg ctggtgttgt atggatggca gatgcagggc cagtccaaga cgtgccccct
      181 gtcccagagg gtgcttgcaa gggatagatg ccgttcgcat gtaggttttt ttcttgacgc
      241 aatccgcgca aaatgctaat ttatgcttgg tgacgcgatc gatccgtcaa catcagggcg
      301 acggaatcac gacgggagcg gcactgcttc ctggtcctgg ggcgtacact cattcctgtc
      361 tttatgcgcg cctgggggcc ggtctcgaag cgcatcatgt ccaaaatcag aaggaggaaa
      421 tgcgatgttg ccagtaggga gagtagcctt cgcagggctt gtgacggtcc tggcggtcgc
      481 gatgatggcc gacgccacgg aggtaagcct gtacagcatt gacggcaggc aaaccggcaa
      541 cgcgaacagg acggggtcgc gacgtcttca agagttcaac ccgagcctga gcatgccgaa
      601 ctccatcggc gggcccggcc aggagtactt tgaccgagtc ggcatgcggt ttggtcttga
      661 tgccggcgag aggatatggg gtgagttgga tctgtacttc tggttgaacg acgtctatca
      721 gacgtacggc caggatcctg cctgccgcaa gcagatcgac gtcacgaaca cctcggacca
      781 gtatgcggac gtctggctcg aactggacct gaacgtactg tcggacccgc cgttcccggt
      841 gcctccgcac ccgtggcctg acccgctggg accggtgccg gccaagggtc gatacctgct
      901 gcgtttcgac tgcagaggaa tgagcggcgg gaattcgcga tacgacatct actggtcgac
      961 gaatggcgat gacggcgatg aaaaccaggc atggcagtgg gactcggggg caagtgcgcc
     1021 gtgggcgtat ccgggtggtg cctgggatgt tcgcgtcaat gcgatgcccg aaccggctgc
     1081 ggcgctgctg ctggttctgg gcggcgtggt gggattgcgg cgacgccgat gatcgtctgg
     1141 tgagagccta tcccaaaacc ctgaaagccg accgggcgga agccggtccc agacaggtct
     1201 tgggatagtc tccaaggcgg accgcctttt tgcgggccgc gagccaagcg tacggcgatc
     1261 cagaaacatg cgcgcaaacc gcacagatat ccggtcagaa agttctaaac ccttatgcgt
     1321 tgggcagata cagtttcgtg gcgtcgagga caggcatggg atacgctcaa cgtttacgga
     1381 gacagcgact cgtgggggag ggttcaggct gagttgggcg gtccataggg tgggatggac
     1441 aggtgagaag ggtgagacgg ggaggaatcg cctcgaagcg tgtttgccca cctccggtat
     1501 ttctggtaga ctgctaggtc tgcaaatgag gccgatccgg gtacttgggc cggcggcagg
     1561 gagcacgcgg cccgccaccc cgatcggtca ttgttatcca ttgggagctg aggttctatg
     1621 gtacgacttc ggttgaagag gactggtcgg cggcatcgcc cgacttatcg cctggcggcg
     1681 gtggacagcc ggtcgccacg ggatgggatg gtcattgagg agttgggggt gtacgagccg
     1741 gccaaccaga acccggatct gcgctgcaag ctgaagcagg agcgcgtgga gtactggctg
     1801 aaggtcggcg cccggcccag tgacacggcg cgtgatctgc tcaaacggct cggctaccag
     1861 gtgagcgtcc ggaagtgagg cgatccggtg cgcatcgacg tgctgacgct gtttcccggc
     1921 gtgatggagc cgttcttcgc ctccagcatc atcgcacggg ccagagcggc gggcatcgtc
     1981 tcgatctggt gcacgaaccc ccgagatttc acgacggatc gacatcgcag tgttgatgat
     2041 cggccgtttg ggggcggtcc gggaatggtg atgatgtgcg gtccgctttt tgacgcggtg
     2101 gagaaactgg agaaagacga tccgaccccg gccgttcgga ttctgctcac gccgcagggc
     2161 gaacgattcg atcaggcgat tgcgcgcgag ctggcacgtg agaaacggct gattctgatc
     2221 tgcggccatt acgagggatt tgacgagcgg atccggatcg ggctgaagcc gagggagatt
     2281 tcgatcggcg attacgtatt gtcgggcggg gaagttgcgg cgatggcggt ggtggacacg
     2341 gtggtccggc ttctgccggg cgccttggga gacgaggcgt cggcggaaca tgaatcgttt
     2401 tcgggcaggg tgctggaata cccgcagtat actcgcccgc gtgagtttcg aggaatgcag
     2461 gttccggagg tgttgctgtc cggcgatcat gcgaagattg ccgaatggcg tgctgagcag
     2521 gcggccaagc gaacggccga gcgaagaccc gacctgctgg cgccggcgaa ttcaaaagag
     2581 gacgacggcc gcggataggc cgacgaggag cgacatcatg atcaacgcgc tgattgagaa
     2641 agttgagagc gcagccaaga agaagcaatt gccgaagttc tcgattggag atacggtttc
     2701 ggtgcaggtt cggatcgttg agggcggcaa ggaacggatc cagccgttta tcggggtggt
     2761 catcggccgt cgggggcggg ggatgggcga gagcttcacg gtccgccgca tcgtgaacaa
     2821 cgaaggcgtc gagcggatct ttccgctgca ttcgccgaag gtggcgggcg tcgagatcat
     2881 ccgctccggc cacattcgcc gagcgaagct gtattatctg cgggatcgcg tggggaaggc
     2941 ccgccgtctg cgggatcgca agcgtgacgc ccggcaggcc gtggatgcgg ccgttcagca
     3001 gtgacgggag cggcgtggcg attggcttcc cggcgggcgt gaaggcagcg tttcgcgggg
     3061 gcacatcttt tcggcaccgc gagcatcgca atcggcgggg cgtctgatgg acgatcgggc
     3121 gagactgggt cgggccgggg agaaggaatc ggagcggtac ctgcgccggc aaggccacaa
     3181 gatcgtctgc cgcaactatc gatgcccggc cggtgaaatc gacctgatca gcctcgacgg
     3241 caagaccatc gttttcacgg aggtcaagac ccggtcggat tccgaacgag ccaacccgga
     3301 agacgccgtc aatccggcaa agcgtcatcg tctggttgcc agtgccaagt gtttcatcca
     3361 gcagacccgt tcgcaggggc gggcctaccg attcgacatc ctggccctga ccgcgaacgc
     3421 caagggcggg ctggatatcg aacacttcaa gggcgccttc tcgtgtaaat gctaaactaa
     3481 tgcattatga actttttgac acccattgcc acctaaccat cgatgacctg tacccccgcc
     3541 gcgagcaggt cgttgcggat gccgcggccg cgggggtcac gcggatgatc accgtggcct
     3601 gtgtcccgga cgaatgggaa acggcgtcgg cgatgcgaag cgattttccg ggcgtctggg
     3661 tcgccgcttc ggttcatcct cacgaggcag gcaaggtcgg tgctgacgat ctcgatcgcc
     3721 tggcaggcgt gtggcgacgg ccgggcgtgg tcgcagcggg cgagatgggg ctggactacc
     3781 actatgattt cagcccgcgt caggtgcagc agttggtttt tgaaaggcaa ctcgaactgg
     3841 cctgggacac cggcctgccg ctggtcattc actgcaggga agcacacgac gacgtcgtgc
     3901 ggctgctcga aaagcacggt taccggggca agcccgttgt gtttcactgt ttcggcggcg
     3961 gaccggagga agcagcggac ctgcggtcgc gcggctggcg ggtgtcgttc agcggaatcc
     4021 tgacattcaa gaaggcccgg caggcccagg cggcctgtgc cgaaacaccg atcgaccaga
     4081 ttctttttga aaccgatgcc ccctatcttt cgcccgagcc ggtgcgcaac agacggccca
     4141 atgagccggc ctacgttgcc tacactgttc gctttgccgc gcaacttcgc ggcgagacat
     4201 ttgagactct cgctgccgcc agtaccggga atgccgtgaa gctcttcgcg ccggctgagc
     4261 acggggatca taagccgtag gtggcggccg ccgtggagca gccgcatgta gaggacaggg
     4321 cgttcagggt atctgagacc tgatcggtcg tcggtccggc ggcccctcgt tgatgttgtc
     4381 tgcgaacatg aatggcgcga atgtcctgtt gtgcagtgtt tcagagcagg taggacccat
     4441 ggctgtatgg gtgcggcgac ggtttgatgc cgggggggtt gcgggatggg cgtcgaggca
     4501 ccaaggcgcg gtcgtggtcg gagtacggca ctgtcggcgt ggagatcgat cttgggagaa
     4561 caatcggatg ggccggccgg gcatgggttg agacggatga ttaaagcatg gatgaaactt
     4621 gggtttgttt ggcgcaggcg gttttgtcga ggggcaaggg gcatggtggc ggagacgacg
     4681 gttggttgca agatcttttt tggcatgagt ttggatatcg ttgacaaaaa gactgccggg
     4741 gttattggga tcatcggttg cctcctgggc gcgccgtgag atcaattcaa ttgaaggccg
     4801 tcgtatagtg tggtcggcgc gcgggatctc cgccgcgttg gttgcggggc ataacacgca
     4861 gggtgttccg gcgaggcggg cgggaacgga gcgggaagca gggcgcatgg caaaggtgga
     4921 gcgtctcttg gcggccgcaa cccaagcgca caatgtcccg gcagaagcat gcatgtgaaa
     4981 tgcgcaccag ttgtgacggt ggaatagact gcgtgtgcca gggttatgcc ttgctgagcg
     5041 ggtttatgtt gcggcttcaa atccgcgcga tctgcggcag gacgtcgaaa tggcgatcac
     5101 ggctgaacaa caccaggtca tgctggatga ccagggcggc tatccaaaga tcgttggtcg
     5161 gtatgggcgt tccggcgaca cggagttgcg tgaacacctc cgcatagaca tgggtggtct
     5221 gctcatcggg caagagcacc gtcacacgat cagactgcag gaaacgagag aggacggctt
     5281 cgttggcctt ggctcgctgg ccggcgcgga agccggcccg caactcggcc aggacaacaa
     5341 acggcaggta gatctggccc gctcgacgta cgcacgcaac cgcctcatcg atgcctcggg
     5401 caaagtccac ataccgattg acgtcgatgg cgattctcag cgccatgttt ccggatctac
     5461 cgtatcttgt tgcctgattg cttcgtcgaa agccggatcg tcctgccaag tgcctgcgag
     5521 gcaatcgaga tcatctgcaa gctggttttc ctcgggaaga ccgactcctc gcttcagggc
     5581 ctcgcgggca acctggttga ggcttttgcc ttcttgcctg gccctctcac ggagggcgcg
     5641 atccagcgcc ggcgggatat ctcgcagtgt gtactggcgg ttcttgcgtt tcatgcctac
     5701 attatgcgca tacgcgtgta tgcagtcaag caggcatcat tgaggcggcg gccgttccgc
     5761 tttttatcgg gcgttgggca caaccacatc tggctgagcc gttgaggttg cggtctcggc
     5821 gggatcagcg caattgcttg tgggagttgc gcttatgttc ggccaacttg ccaaaatgtc
     5881 gcaggcctcg tcctgcagct tggtgaacgg cttgacgtcc gagtcgtcgt cgcttcgttc
     5941 ggggatctcg ttgtagacga acgagaatgc cagccatttg ccggcgttgg ttttgatgta
     6001 gccgctggtt gaggacacgc cgccgatgta gccggtcttg cccaggacgc ggcctttgag
     6061 gtcggtcatg cggtccttga gcgagccgtc cacgccgcag atggtgaggc tggccatgta
     6121 gtcttcacga tccggtcgtg cgtacatcac tgccagaagg tcggtcagca tccgggtggt
     6181 gaggcggttc tgcgggctga ggcctgagcc gtcgattggt acaagtcggc ggtcgtcgat
     6241 gccgttcttt ctcaggaagg cgcggagagc ccgcccgccg ttttcccagg agccgggcac
     6301 gcgccgccca tgcttggcct cgtatgcctg gccggtcatt ttgcacaagc actcggcgaa
     6361 gaggttttgg ctgttcttat tgatccgaga caggatgtcg gacattttcg tttcgtggac
     6421 ggcgatgatc ttgtcgcaag gcggggggat cgcgccgccc aatggtttgt cggcctttcg
     6481 gatgcggccg tcgacggtga tgcccctggc cgccagttgc gtacgcaggg cgtctgcgaa
     6541 gaacgctccg ggatcatcga ccggcttgct cttgagttcg gtcatttccg agcaggtgcc
     6601 gccgatcttg tagatgttgc cctccttgag cttggcgatg gtcggagcgt ttttgtcagc
     6661 ggtcatgcac tcgttgatga ctttgacgca ctgggccggc ggcatgactt catatcgggg
     6721 cggcttgccg ggctgagtgg ggtaaatggt gatatccacg ctgttatcgt tgaagttcag
     6781 gccggcggtc ggagcggcat accagtgaag cagccaatgc ttgggccaag tcgggtggac
     6841 cttgggagac tcctcgaacg cggagtcgtc atatacgatg tcgcctttga tcctgcggat
     6901 cccgcgctgc tcaatcgccg tgacccagtc ttggagcatg gaaacgggtg tgcggccggc
     6961 ggccttggcc aatcgcgggt cgccgcaagc ggggtcgccc gtgccgatca gccagaggtc
     7021 gtcgccgtcc atcgccaggt atgtcttgac cttgtagtcg gccccgaaca tgtccagccc
     7081 ggcggcgctg gtcagcagct tgaagttgct cgcgggcgtg cagggcttat cgatccggcg
     7141 ggcgtagatc tcgcaccgac tgggcagttc gagcacccgg gcatggatga ccacaccctt
     7201 gtccgcatat cgatcgagta ccggatcgag ccgagcggcc agtttcggat catgggcgat
     7261 ccgggggctg gagcagccag tcaggatcaa caagaggccg gccagcccaa accggtatgg
     7321 atggcatgtt ctcatttctg tcctctctcg gtatttactt gaagatcatg ctacagatcc
     7381 agattgccat cagaactccg gcggcgtcgg ccagcaggcc ggcgatcatc gcgtggcgga
     7441 tcttgcggat tccgacggag ccgaagtata ccgcgatgac gtagaaagtc gtgtccgtgc
     7501 tgccaaagac cgttgaggcg agttgcgact cgaaggaatc cggcttggcc tgaatgacct
     7561 cggcggccag tccgagcgag ccggagccgg acagcggccg catcagggcc acgggcagca
     7621 cctcgggcgg catgccgatc tttagggtga gcggagcaag acactcggac agaaactcga
     7681 ggccgccgga gacacggaac gtgccgatgg ccacgaagat agccacgagg tagggcagga
     7741 tccggatgaa gacgtcgaag ccttccttcc cgccggtgac gaaggactca tagacttttg
     7801 cccgcttgat catcgcgtaa ccgatgatga gcagaaagat caccgggatg gtgaacatcg
     7861 agatctggtt gagaatgagc ttgaaggtca tcatgacggt tgctcctttc cgctcttggc
     7921 gaccgccgca tcggtctgtg gctcaatccc ctcgcgttgg gcctcggcca gggccgcgtt
     7981 gagcgagaac atggggagcc gcccgagcag ccgggctccc aggatggcca cgatcgtcga
     8041 tgtggccccg gcgatcatgc aggtgccgat gatctcgaat gggtcgctgc acccggcgga
     8101 cttgcgcagg gccaagagcg tggtcggcag gagggcgaag ccggccgtgt tgtaggccag
     8161 gagcatgacc tgcgagtcgc tggccacgcc cttgtgcggg ttgagttcct ggagttgctc
     8221 cattgccttg aggccgaacg gcgtggcggc gttgcccagg ccgaggatgg tggtggtcat
     8281 gttcatcagg atggccccgt tggccgggtg gtcgcggggc acctgcggga acaggagccg
     8341 gatgatcggg tgaaagacgt gtgcgacaag ctgcacgatg cctgcatcct cgccgacctt
     8401 catcaagccg aggaatagca ccatcgccgc gaccaacccc aggcagatct cgaaacctct
     8461 ctgggcatag tcgaaggcgg catcggtcat ggactgaagc gtcaccagcg agccgcgccc
     8521 gacggacctg tcgttgacca ccacctcata cttgccggaa tcgtcgctgt cccacttgag
     8581 cagatggccg ccctggacct gtctggccga gataagccac ggccggccct ggtccttagc
     8641 cttcgcgtcg gcaggcaggt caagaccttc gggtccaagc cagagtcgga aatcgaacga
     8701 tgccgcgcgg tcaccggaat ccttgatacg cacggtcagc atgttgagcc cgtgtcggac
     8761 cgcatcggcc gggataaccg tggttgtcgg caggtagtgc tgcgccacac acaacccgaa
     8821 tgccgccagg atcatcaggc cccagacagt gttgatgttc agcaggtaac gaatgatgcc
     8881 gcccagtatc ctcatgagta tgggcctcct gaaagcgaac gcgttcggca gcggccggcc
     8941 cggccgtcgg gtatagagag tatcagactt gtccgttttt gccgagcggc agtggcggcc
     9001 tgttccaggg gtgccacgat cggcttgcct gcggggtacc gctggtggct tgtctgtcgg
     9061 tgcggagcca acctatcgtg gacagcacca gttacagggc aaccgctctg aggccggctt
     9121 gtgtggcacc ttgtttcggc cggacgaggt gcactgaaca ggccaggcac gggtcgaagg
     9181 agtggatcac gcgcagggct tcgatcggcc gtgaggtatc ggcgatgggg gtgccgatga
     9241 gggcttgctc gatcgggccg ggctgattgg cgtcatcgcg aggtgaggcg ttccaggccg
     9301 ttggggtaat gacctggtag cgggcgatct tgcggtcttc gatgcggatc cagtgaccca
     9361 gtgctccccg cggggcctcg gtcaggccga cgccgccggc agaggcgggc acgggcttct
     9421 ggacacaggc agtctcgccc ggaatcaact gactgagcca cacatccatc gcgtcggcga
     9481 cctgcttggt ctcaagtgtc cgggcgatca gccgatccat caccgagatg ccgcgacgat
     9541 agcggccgct gatccacatc cgggcgagcg ggccgagttc gtggacgata ttctggtagc
     9601 gaggcgactt gatccacgaa taggcacccg gcttgtctgc gtccggcgcc gtgttgcccg
     9661 aggccggatt gagagtgccg caatcaggcg tgtaccacga gtgtttgact gattctgtga
     9721 tgtccgattc ggcgaacgag tgtaactctc cattggtgta gcggccgctg ggcagcagtc
     9781 tggcagaacc actgttatcc agatcgaaac cgccgcaggc gagcagattt ccgcagccgg
     9841 taccaatctg gaagtaagcg gggaacagag tgcccaatgt ccgggcgtcc ggcacgagcg
     9901 cgctttcgac gaacacacgg acttcggcga gcagagagcg aaacgcgctg atcttgtcgt
     9961 tggtgaccac gtcggtcgct ccgccgacca cgaagctggc cgcacagggc attcgtccgc
    10021 cgaagatggc gcccatctga tgagctttgc ggcgcatcgc cagcgcggtg atgtagtgtt
    10081 cgaccaaggt ccgcgcggtt gcaccgtcga tcatgtcagc agcggtgtaa cctgggctcc
    10141 acggcggctt gtccaggatg ccggttgtgt tgatgtagtc cagggctgcc aggtgataga
    10201 agtgcaggat gtgcgagtgc aggaagtcgg tgcccaggac gaggttgcgc aagatacggc
    10261 cgttgtgcgg cggcttgatg ccgcaggcgt cttccagggc catcgccgag gccatgccgt
    10321 gcccgatcgg acagacaccg cagatgcgct gcgtgtaggg cgttgcgtcg cgcgggtcgc
    10381 ggccgatcag cagggtctcg aacccgcgga acatcgtccc gctgctgcgg gcgtcgacca
    10441 cctgctggac gccattgacc gtgtcgacgg tcagctcgac tgccatgtga ccttcgatcc
    10501 gggtgatggg gtctatcttg accgtcgtcg gcataggtcc gcccttcgtc agcctgctct
    10561 tcattcaccg gagtcgaaga aagaactcat gcccggaaac cccggattga cgcatccgag
    10621 acaggggccg cctgagccga tacaccagtt cacaccaaag cgccccggcc ggccggtgtt
    10681 ccacttacgc aggtagcagt cggcataggt cgactcgccc cggcaaccga gattgatcag
    10741 gcagccgtcg ctgcccagtg agccggccat gcactcgtcg ccgttcttgc agcgcctctc
    10801 gcagtaatcg tggattcgat tctcgaagta caccatcggc cggcggttgg catccaacgg
    10861 cggggcctgg ttgtgtttga gcagatgggc gatggtgccc acgatccagt cggggtgcga
    10921 cgggcagccg gggatgttga taatccgagc gtcgtcaccc aggataccgc ggacgctctt
    10981 ggcctccgtc gggttggtgt tggctgagac cagaccgccg aacgcagcgc agcttccgac
    11041 ggcaaggatg aaccgagccc cgggtgcgaa gtcgaccagg gcctgctgca tggtcttgcc
    11101 ttcccatacg gtgcagaacc tgcccgccgc acccgtcggg atggcccctt caatcacgag
    11161 aatgtagccg cccagaccgc gtcgcttctc ggcagccgac accgtcagtt cgcccgccgc
    11221 cgccgacagg gtggggtggt actccagatc gatcacgttc tgcagcaggt cgtcggcggt
    11281 cgtgtaataa atgctgttga gcagcgaggt ggagcaaccg ctgcaaccct ggccttgcag
    11341 ccagatgacc ggcggatgcg acttgccggc cagcacctgc tgaagctgag ccacggccct
    11401 gccggcaagc cctggggcgg ccagcaccgc ggcagaggcc ttcaacaagt cgcgccggct
    11461 gatactcatt tgatgtcctc ccagtctcta catgtagctg aacgccggct gggacacaag
    11521 catttcatgt tcgaggcgat gctcaattca tgcagctcgc ggatggcgag gggtgtggag
    11581 ccggcgaggt gtcagtcagg caggacatcc gtcccgatgg tgtgacacgg ccggcaagaa
    11641 accttgcctg gcatgcgctt gcggtgctac ttgccttcca gccagttgtc gccccagttg
    11701 atgtccacct tgatcggcac cttgagcggc agggcggtgc acatctcggt gcggatgaac
    11761 tcggcctccg cctcgacggc cgagcgcggc acgtcgaaca cgagttcgtc gtggacctgg
    11821 atgagcatcc tagaggggcg ttgttcggaa cggattcgct gatggatgcc gatcatcgcc
    11881 cgtttgatca ggtcggcggc cgaaccctgg atgacggtgt tgacggccac gcgctcgccg
    11941 agagagcgca tgcccttgtt tcgcgagttg atctcgggca cctgtcgccg ccggccgagg
    12001 attgtctcga catagccgcg ttttcgggtc tcggcgaccg tcttgtcgat gaacatgcgg
    12061 atgccgggat atcgcatgaa gtacatgttg atgaacgact gagcctcggt gacgctcatg
    12121 ccggtggttc gcgaaagccc gtaagctccc tggccgtaga tgatgccgaa gttgaccgcc
    12181 ttggcccggc ttcgctgttg cctggtgacc tggtcgagtg gcacgccgaa aacctgggcg
    12241 gcgacaaact ggtgaatgtc gcggtcctcg atgaacgcag tccggagggc ctggtcgccg
    12301 cagaagtgag cgaggacccg aagctc
//
LOCUS       DAOTPW010000173        12161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5001_length_12161_cov_4.905171, whole genome shotgun sequence.
ACCESSION   DAOTPW010000173 DAOTPW010000000
VERSION     DAOTPW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12161
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5001_length_12161_cov_4.905171"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="P5316_15015"
     CDS             <1..317
                     /locus_tag="P5316_15015"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564228.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRR86316.1"
                     /translation="TISGLMIGMGETGDEIGQTLSHLREVGCGIVTIGQYLQPSKEHW
                     PVVRYYTPEEFEELAVKARAMGFAAVAAGPFVRSSYNAGKVFSGTAIGSSGGTAGQLP
                     GD"
     gene            complement(299..2230)
                     /locus_tag="P5316_15020"
     CDS             complement(299..2230)
                     /locus_tag="P5316_15020"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HRR86317.1"
                     /translation="MPTRRTLAFSIAPYVLSTMLPVLASWSGSMAEQPSLAPEFPGVL
                     PKPKDYRENGPPRLLVRSSGVLRIQIFGKHAPLSAGRQELIEALQDLGVRAEANNEGA
                     RVRIGINADWPGGTAHLPKPTGERPQAYALTVTTAEDGKTTVAIRATDAIGAYYGIRT
                     LIQLLDRTPEGVTIRQAQVRDWPTFTLRMFKGQCWYYRDNLMFVRWAPQFKWNTFGIC
                     YTDCPDWRSPPESYRTMVADACKTAASQDTMQIMQLGNPYMLKEKAIRATSDTDIETL
                     ATFFELSLANGSTALMLCLDDFAFLPDEDRSQFENLAGANASIVKRFAERIWAKHPDT
                     RILLCPPPYWLTANKARGYQWAHDYLRDLCANIPPQISIVWTGREVTTVCHETADIEA
                     YQELIGPPRRLFLWDNTLKMPPGWGNVFRMNAFLETCDHIATSAWPKMADYVHGAAGI
                     NTYGPAEIYKVPLMTAADYLWNPEEYDPKDSLRRALYWFDTNHEVGPMVHRWVNDLHQ
                     TLFDKRLAFLKSPTEAGLKEINELTARYRVEFDRIASMTSNKALVETLQPYLRRHTEA
                     TAVLSTVLDGWSTRNTDKAATKDKLTRARTAFDRLAKTLEKGDQAGRQHGLVRQELEQ
                     ATRNAVDALIANVAQSPGN"
     gene            complement(2518..3966)
                     /locus_tag="P5316_15025"
     CDS             complement(2518..3966)
                     /locus_tag="P5316_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86318.1"
                     /translation="MKETLSRRGFMSAAAAGTGAALFGRTVMGQTATDRKADGRTAYP
                     PVKIHRVYVGRQRAAWPKPAFKPEDEVPVFERKLAELKARMGDVELVGGELVQTAEEA
                     AKLIPKLKEADGVLLIHLALGVLPILQQIVDAGRPTAFFSQPFSGHEWMFVQQWQNAG
                     KKVALFATRDLNELADAVNIVAVPARMARSRLVVVGRPAGTPPAIVPERVKEKLGVEV
                     IPVSVEEVIEAHKSVDPKLAEAEAEQIWIKQARKIIEPTREEIIKSSKMFLAMKAILT
                     ANRAQAITINCLGGIPIDILGYPCLGFTRLLDMGLVGACEADMDSSLTMLIFTYAFGV
                     PGFISDPLFDTSKNAVIHAHCTAPTRMDGPAGERAPFIIRTHTDDDKGAALEVEMRLG
                     QVITCAKLVNLDTMIASTGKIIEIPDFDDRGCRTQITTEVANAAAMLANWGAGLVDGW
                     VPQLHRVVFYGDRLAGVRQLAPLMGFKVIEGI"
     gene            complement(4477..5448)
                     /locus_tag="P5316_15030"
     CDS             complement(4477..5448)
                     /locus_tag="P5316_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRR86319.1"
                     /translation="MSTRFRIAERLPELVNRVMTSYNEDERTRHLDHVYLPGRAEIIE
                     IIHLLLELAYPGYHGRQNLNQHNVTFHVGELLPKLGEKLHEQIFRAICHKRRTECNED
                     CAANRCEQDARDMTMRFIETVPAIREMLAGDVQAAYDGDPAAASMDEIIMSYPGVLAI
                     TVYRFAHELYLMDVPLIPRIMNEWAHSKTGADIHPGARIGRNFFIDHATGVVIGETTE
                     IGDNVKLYQGVTLGALSFPKDARGRLIRGHKRHPTVKNNVTIYANATVLGGETVIGEG
                     AVIGASVFLTSSIPDNATVRMKDPELTVRKREPTDTRAEPWKLDFQI"
     gene            5979..7181
                     /gene="larC"
                     /locus_tag="P5316_15035"
     CDS             5979..7181
                     /gene="larC"
                     /locus_tag="P5316_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRR86320.1"
                     /translation="MRIAYFDCFSGASGDMILGALLDAGLCLNELREDLASLGIGGYA
                     LAAERIRKQGFAATQFEVRIEAADKPHRHLKHIREIIENGRLPSAVRERAMAIFTRLA
                     EAEAEAHGTSVEKVHFHEVGAIDSIVDIVGACVALDRLGIEEVHCSPIPTGGGTLCCD
                     HGVMPVPAPATARLLKGVPLAACEEMSELTTPTGAAILTTVARSFGPLPAMVTDRIGV
                     GAGKREGKTRPNILRVLIGEAVGASASDDESDEVLVLEANLDDATGEVVGHVYDLLFA
                     AGALDVYSSPIYMKKNRPATLLTVLAPPPLRETIEGILFAETTTFGIRSHLASRHKLA
                     RTFETVETGFGPVRIKVGRRRGRVVTASPEFEDCREAAKRSSLPLREVMELAMRIWRS
                     RSGGCEPV"
     gene            7212..7784
                     /locus_tag="P5316_15040"
     CDS             7212..7784
                     /locus_tag="P5316_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86321.1"
                     /translation="MLGEGIRETLSRILESQNLFPLALLVALTTYLLWTSRRKLRQHA
                     NQPKRSTPQREPEPVSPGSKEQLRRDLESLIAELEQLSRKISAEIDTRFAKLEAAMHD
                     ADRRIAALHRLTQQQEGSTARMPAREESEGAKPASDRPEDRYALIYELTDVGFSPVEI
                     ARDLGRTPGEVELILNLRNRGRPKAPDSPS"
     gene            7871..9316
                     /gene="cls"
                     /locus_tag="P5316_15045"
     CDS             7871..9316
                     /gene="cls"
                     /locus_tag="P5316_15045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRR86322.1"
                     /translation="MEPWWFWLLVAVHETTVVVAVVTVLRWRKEPRSMLAWILAVRLL
                     PVLGILFFLVFGEPRGGWHRLRRRRRRKRLEAALAHKWGVFQQQHPASPLSITDPVVK
                     EFARLTKRLGGHIAAPGNAVRVFNDAEETYAAIEKAIDEAQSHVHMEYYIFQPDKTGQ
                     AIRDRLIRKAREGVRCRLLLDYIGCWNLSRRFVRPMREAGVEVAYAMPVIPWHGRWRV
                     NYRNHRKIVVVDGRIGFTGSQNIGDEYRGRLARYGPWRDTHLMIQGPCVHHLQEVFIE
                     DWHYTTREDLVKDEYFPLPGPAGEHMVQLLPSGPGQPIRVMHHLLFAAVSAARSSVSV
                     ITPYFVPDAAMVLALQSACYRGVRVRLLIPTTTDHHFVLFAGRSFYPELMEAGVEIFE
                     HDATMLHSKVMIIDRAFALVGSANMDERSFRLNFELTVMLYSPTVADDLYKDFETLTA
                     RSRRIRSRHFGKHSFAREMATGLARLASPLL"
     gene            complement(9535..10656)
                     /locus_tag="P5316_15050"
     CDS             complement(9535..10656)
                     /locus_tag="P5316_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR86323.1"
                     /translation="MPHPGPIPIPESGSDQAHPLRLVFWETTAGCNLECSHCRRLDVS
                     RELMRTDLTPTEGKRLIDQIAETGRPVLIFSGGEPLMRPDIFELATHARNRGLMIALA
                     SNGTLIDEPMARTIAQSGFDRVSISLDGADADTHDRFRMQTGSFDRAIAALAHLRTLG
                     VQTQINCTVARHDKDQIEAVLRLAEQAGAAAVHYFLLVPVGCGEQIADDQMLDAKEVE
                     DRLRLIDNLQQTTSLQIKPTCAPHYYRVIRQQAREQHRPRSGQTRQSALHTMTRGCLA
                     GIGVCFVSHEGKVFPCGYLPLEAGDVRRTSFGAIWRNSELFHALRDFSRLTGKCGICP
                     FRNVCGGCRARAFYEFADYLAEEPFCAYEPGQEAKTDEG"
     gene            10845..>12161
                     /locus_tag="P5316_15055"
     CDS             10845..>12161
                     /locus_tag="P5316_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86324.1"
                     /translation="MEQDMSIADSFRLVWTPFLKKQSGTMMQAVGLVCPPVLGSGGEL
                     GLRVGDWQQAARKIDLISYPEHLHTLDKPRTYLWLVPRPRRPVAITALWRTSLGKSYR
                     ASLLLHPAEPREICVVFKTHIDLGYTATFQEVLRLYRTRLLEQLLENLDRTANRKAGR
                     RFIWMLSTWLLEQCLDPRRTRREYIRRLEHYIRAGQVVWGLMPFTTHSEFFGIEEMCR
                     SIYAARRLAERYGVPVPQAAKMTDVPAHTSALAMAFAAAGGRFFQIGTNPDSRPPEVP
                     PLFWWALPDGQRLLCHYHATYGTPLLPPQDWPWKNWLSVQMTSDNVGPQNLDLLDHMD
                     WIEQHFDWPVCRTGRLEDFATAVEHDHGDELPVFGKELTDWWIYGIASQAGPTALAKR
                     TKERLAAVETLATAAAMSQSKDMDAGLRSLFVQAYEKLALYTEHTWG"
BASE COUNT         2083 a         3499 c         4105 g         2474 t
ORIGIN      
        1 cgaccataag cggcctgatg atcgggatgg gagaaaccgg cgacgaaatc gggcagacac
       61 tgagtcatct gcgggaggtc ggctgtggca tcgtgaccat cgggcagtac cttcagccat
      121 ccaaggagca ttggccggtc gtccgctact atacgcccga ggaatttgag gagcttgccg
      181 tcaaagcaag ggcaatgggc ttcgcggcgg ttgccgccgg tccgtttgtc cggagcagct
      241 acaacgccgg caaggttttt tccggcacgg ccatcggttc gtccggaggg acagccggtc
      301 agttgccggg tgactgagcg acgtttgcga taagggcgtc gacggcgttg cgagtcgcct
      361 gttcgagttc ctggcggacc aagccgtgct gccggccggc ctggtctcct ttttcaagag
      421 tcttggccaa gcggtcgaaa gccgtcctgg cgcgggtcag tttgtccttg gttgccgcct
      481 tgtcggtgtt tcttgtcgac cagccatcca gaaccgttga gagaacggcc gtcgcttcgg
      541 tgtgacgtcg caggtaaggt tgcagcgtct caaccagcgc cttgttggat gtcatcgatg
      601 cgatgcgatc aaattcgacc cgatacctgg cggtcaactc attgatctcc ttgaggccgg
      661 cttcggtcgg cgatttgagg aacgccagac gcttgtcgaa gagcgtctgg tgaagatcgt
      721 tcacccagcg gtggaccatc ggtccgactt cgtggttggt atcgaaccaa tagagagccc
      781 ggcgcaagga gtctttcgga tcgtattcct cggggttcca caggtagtcg gcggcggtca
      841 tcagcggcac tttgtagatc tcggccggtc cataagtgtt gatgccggcc gcgccgtgga
      901 cgtaatcggc catcttcggc caggcgctgg ttgcgatgtg gtcgcaggtt tccaggaacg
      961 cgttcatgcg gaacacgttg ccccagccgg ggggcatctt caacgtgttg tcccagagga
     1021 acaggcggcg gggggggccg atcagttcct ggtaggcctc gatatcggcg gtttcatggc
     1081 acacggtagt cacctcgcgg ccggtccaga cgatggagat ttggggtggg atgttggcgc
     1141 acaggtcgcg caggtagtcg tgagcccact ggtacccgcg ggccttgttg gcggtgagcc
     1201 agtagggcgg cgggcagaga aggatccggg tgtcggggtg tttcgcccaa atccgctcgg
     1261 cgaaacgctt gacgatgctg gcgttggccc cggcgaggtt ctcgaattgt gatcgatcct
     1321 cgtcgggcag gaatgcaaag tcgtcaaggc agagcatgag cgcggtactg ccgttggcca
     1381 gcgacaactc gaagaacgtc gccagcgtct cgatgtcggt gtcggaggtt gcccgaatgg
     1441 ctttttcctt gagcatgtag gggttgccga gttgcatgat ctgcatggtg tcctggcttg
     1501 cggcggtttt gcaggcgtcg gctaccatgg tccgatacga ttccggcggg ctgcgccagt
     1561 cgggacaatc ggtgtagcaa ataccgaacg tgttccattt gaactgtggg gcccaccgga
     1621 caaacatcag gttgtcgcgg tagtaccagc actgaccctt gaacatgcgc agggtgaagg
     1681 tcggccagtc gcgtacctgg gcctgcctga tggtgacgcc ttcgggcgtg cgatcaagca
     1741 actggatcag cgtgcggatt ccatagtatg cgccgatggc gtcggtggcg cgtatggcca
     1801 cggttgtctt gccgtcctcg gccgtggtga cggtcagggc ataggcttga ggccgttcgc
     1861 cggtgggctt tggcagatgg gcggtgcctc ccggccagtc ggcgttgatg ccgatgcgaa
     1921 cgcgagcacc ctcgttgttg gcctctgctc ggacgcccag gtcctgcagt gcctcgatca
     1981 gttcctgacg gccggcggac aggggagcgt gctttccgaa aatctggatg cgaaggacgc
     2041 cgctggagcg gaccagcagg cggggtgggc cgttttcccg gtagtccttg ggtttcggca
     2101 gaaccccagg gaattccggc gcaagtgacg gttgttcggc catcgaacca gaccaagagg
     2161 ccaagacagg cagcattgtt gacaacacgt atggagcgat gctgaaggcc agcgttcgcc
     2221 gggtaggcat gtttgtcacc tccgggctgt ggttgccgcc cgccggctct tcggcgatgg
     2281 ggggcgccga acgcggccgc cggcgggcat cggtggctgc tctcaggttg accgaaaacc
     2341 gcgagaagat caaccccggc attggatgcc gcaacgtcgg ggagtcaaga atgatgaatg
     2401 gtgcgacgag aatgataggt gcggccccgt tgcctgccaa gggcaatact ggtgattttc
     2461 ggatggtaat gggtctgccc gctgcgaaca agctcagatt cgccggattt cggacggcta
     2521 tatgccctca atcaccttga agcccatcag cggtgccagt tggcgcaccc cggccaggcg
     2581 gtcgccgtag aagaccaccc ggtgaagttg cggcacccag ccgtcgacca gcccggcgcc
     2641 ccagttggcc agcatcgccg cggcgttggc cacctcggtg gtgatctgtg tccggcagcc
     2701 acggtcgtcg aagtcgggga tctcgatgat cttgcctgtc gaggcaatca tcgtgtcgag
     2761 gttgaccagc ttggcgcaag tgatcacctg cccgaggcgc atctcgactt caagcgccgc
     2821 tcccttgtcg tcgtcggtgt gcgtgcggat gatgaacggc gcccgctcgc cggccggccc
     2881 atccatccgc gtgggggcgg tgcagtgagc atggattacg gcgttcttcg aggtgtcgaa
     2941 cagcggatcg ctgatgaagc ccggcacgcc gaaggcgtaa gtgaagatga gcatggtcag
     3001 tgatgagtcc atgtccgcct cgcacgcccc gaccagcccc atgtccaaca gccgcgtgaa
     3061 ccccaggcac ggatagccga ggatgtcgat cgggatgccg ccgagacaat tgatggtaat
     3121 ggcctgtgcc cgattggcgg tcaggatggc cttcatcgcc agaaacatct tggatgactt
     3181 gatgatctcc tcgcgtgtcg gctcgatgat cttgcgggcc tgcttgatcc agatttgctc
     3241 ggcctcggct tcggccagtt tcggatcgac gctcttgtgt gcctcgatca cctcttcgac
     3301 ggaaacgggg atgacttcaa cgccaagctt ttccttgact ctctcgggca cgatggcggg
     3361 gggcgtgccg gccggccgtc cgacaaccac gagccgcgag cgggccatcc gggctggcac
     3421 ggccacgata ttgaccgcgt cggccagctc gttcagatca cgggtggcga acagggcgac
     3481 cttcttgccc gcgttctgcc attgctggac gaacatccat tcgtgcccgc tgaacggctg
     3541 cgagaagaac gcggtgggcc gtccggcatc gacgatctgc tgcaagatag gcaggacgcc
     3601 gagtgccagg tggatcagca gcaccccatc ggcctccttg agcttcggta tcagcttggc
     3661 agcctcttcg gccgtctgaa ccagctcccc accgaccagc tcaacgtcgc ccatacgtgc
     3721 cttgagctcc gccagcttcc gctcgaacac cggcacctca tcctcgggct tgaacgccgg
     3781 tttcggccag gccgcacgtt gccggccgac gtagacccgg tggatcttca ccggcggata
     3841 cgccgtccgt ccgtccgcct tgcgatccgt tgcggtttgg cccataaccg tccggccaaa
     3901 caaagccgcc cccgttccgg ccgccgccgc actcatgaag ccgcgtcgag agagtgtctc
     3961 tttcatgtcc ttggtcctcc tggtgggtgg ccgggggcag atgcctgtgt gctcgattgg
     4021 atccggggtt gactcccggc tttccagaca gtctatcaga tcgaagcctt tccgggaaac
     4081 tccaaagtgc tgccgacgat tgtgtcggca tggtcgcacg gaccggcgga gcacgcgcga
     4141 cacgtgcggc cggtcgggcg acatgagccg cgcggatggt gtgcttcccg gccgggcgca
     4201 agtggcgaag acctgccagt cttggctgca gaacatggca ggtcttggtc gccacggcaa
     4261 cctcgacact gatctactga ctggcgccct cggtttcgca cacattccaa gcgagttttc
     4321 ctgctttgtc ttcagagacg gcatgtcaat gtatttgggg catgccgatg cagagccgtg
     4381 ggccaggcag gcggtagagc cgcaggcatg gtccccggcg tcttgtgcat gtagggcacc
     4441 ggagaccccg gaatgccatc tgctgaccgc tgaacctcag atctggaagt ccagtttcca
     4501 gggctcggct cgggtgtcgg ttggttcgcg cttgcggacg gtgagctccg gatccttcat
     4561 tcggacggtg gcattgtccg gaatgctgct ggtcaggaag acggatgcgc cgatgacggc
     4621 tccttcaccg atgaccgttt cgccaccgag gaccgtggca ttggcgtaga tggtgacgtt
     4681 gttcttgacg gtcggatggc gcttgtgtcc gcggatcaga cggccacggg cgtccttcgg
     4741 gaacgacagc gctccaaggg tcacgccctg gtagagtttg acgttgtcgc cgatttccgt
     4801 agtctcgccg ataaccacgc cggtcgcgtg atcgatgaag aaattgcggc cgatccttgc
     4861 cccggggtgg atatcggctc ccgtcttgga gtgggcccac tcgttcatga tccgcggaat
     4921 gagtggcaca tccatcaggt acagttcatg ggcgaaacga tagacggtaa tcgcgagcac
     4981 gccggggtag gacatgatga tctcgtccat gctggcggcc gcggggtcgc cgtcgtaggc
     5041 ggcctggacg tcgccggcca gcatttcgcg aatggcgggt acggtctcga tgaaccgcat
     5101 ggtcatgtcc cgcgcgtcct gttcgcatcg gttggcggcg cagtcttcgt tgcactcggt
     5161 gcgtcgtttg tgacagatgg cccggaagat ctgctcgtgt agcttctcac cgagcttggg
     5221 cagcagctcg ccgacgtgga aggtaacgtt gtgctggttg aggttctgcc ggccgtggta
     5281 gccggggtag gccaattcga gcagcaggtg gatgatctcg atgatctcgg ctcggccggg
     5341 cagataaaca tgatcgagat ggcgggttcg ttcatcctcg ttgtagctgg tcatgacgcg
     5401 gttgaccagc tctggcagtc gctcagcgat tctgaacctg gttgacattg gtgtctctcc
     5461 gtgtttcctg accgaggtca gagtttacgg gattctagca atcgcaatgc ctggcgcgta
     5521 gtcggttgtc gggaggtgcg tgacagtctg ggcgggcatg ccgaccggtc caggttgggc
     5581 gatcaacctc cttcaggggg gccgccaaag gccggctgac aggccgaccg ttatacggcc
     5641 ggttggtggt cgcccgcggt tcttcttcct ttccatggtc tcggcccgtt tcagagggct
     5701 tgatggggta acacagagaa ggaggaatcg gcccactgtg aaccagtcgt aaaacgaccg
     5761 gcctgattgc ccttcaggaa agaccgctga agcggttctg ctttcggctt gcgttcgcca
     5821 caaccgggtc agccgcgggg agtgccgccg cttctgtcgc tggcccgttg tcggggcggt
     5881 caattgatgt cgttgcaaac cgcgcgacgg cggcatagac tccgtaggag tccaccagaa
     5941 ggaacacggc cgagtgtcag ccgtccggga agggtttcat gcgtatcgcg tatttcgatt
     6001 gttttagcgg ggcgagcggg gacatgatcc tcggcgccct gctggatgca ggactgtgtc
     6061 tgaatgaatt gagggaagac ctggcctcgc tgggcatagg ggggtacgca cttgcggccg
     6121 agcgcatccg aaagcagggc ttcgcggcca cgcagttcga ggttcgcatc gaggctgccg
     6181 ataagccgca ccggcacctg aaacacatcc gcgagatcat cgagaacggg cggttgccca
     6241 gtgctgttcg cgagcgggcc atggccatct tcacgaggct ggccgaggcc gaggccgagg
     6301 cgcacggcac cagcgttgag aaggtccatt tccacgaggt tggggcgatc gattccattg
     6361 tggacatcgt cggagcctgc gtggcactcg atcggctggg tatcgaggaa gtccactgct
     6421 cgccgattcc gaccggcggc ggcacgcttt gttgtgatca tggggtgatg ccggttcccg
     6481 caccggcgac ggccagactg ctcaaggggg tgccgttggc cgcctgcgag gagatgtctg
     6541 aactgaccac gccgaccggg gcggcgatcc tgacgacggt ggcccggtcc tttggtcctc
     6601 tgcccgcgat ggtgacggat cggatcggcg tcggtgccgg caagcgtgaa ggcaagaccc
     6661 gcccgaatat tctgcgtgtt ctgatcggcg aggcggtggg ggccagtgcg tccgacgacg
     6721 agtccgatga ggtgctggtc ctggaggcca accttgacga cgcgacggga gaggtcgtcg
     6781 gtcacgttta cgacctgctg tttgccgcag gtgcactgga tgtttactca tcgccgatct
     6841 atatgaagaa gaatcgtccg gcgacgctgc tgaccgtctt ggcgccgccg cccttgcgcg
     6901 agactataga gggcatcctg tttgccgaga cgacgacgtt cggcatccgc agccacctgg
     6961 cctcgcggca taaactggcc cggacttttg agacggttga aaccggcttc ggccctgtcc
     7021 ggatcaaggt cgggcgccgg cggggccgcg tggtcacggc gtcgcctgaa ttcgaggatt
     7081 gccgcgaggc ggccaagcgt tcgtcgctgc ccttgcgcga ggtcatggag ctcgccatgc
     7141 ggatctggcg gtcaagaagc ggtggttgcg aacctgtctg acctgcgggg accgatatag
     7201 ggtgcataca cgtgctcgga gaaggcatca gagagacgct gagcaggatt ctggagagcc
     7261 agaatctttt tcctcttgcg ttgcttgtgg ctctcacgac gtacctgtta tggacgtccc
     7321 ggcgcaagct gcgacaacac gccaatcagc cgaagcggtc aacgccgcag cgggaaccgg
     7381 aaccggtttc cccgggttca aaggagcaac tgcgccgcga tctggagagt ctgatcgctg
     7441 agctggagca gctttcgagg aagatcagcg ccgagattga tacccggttt gccaagctgg
     7501 aagcggcgat gcatgacgca gacaggcgga ttgccgccct gcaccgactg acgcagcagc
     7561 aggaagggtc gacggcccga atgccggccc gagaggaatc agagggagcc aagccggcgt
     7621 ccgatcggcc ggaagatcgt tatgcgctga tctatgaact gacggacgtc ggattctcac
     7681 ctgtcgagat cgcccgggat ctgggccgga cgccgggcga ggtcgagttg attctcaatc
     7741 ttcgcaacag gggccggccc aaggccccgg actcaccatc ttaagctgtt cgcggcgcaa
     7801 acacgtgccg ccggctggcc cggcggttca cgcggactct gagggaacga gccggtctgg
     7861 gaagaatcgg atggaacctt ggtggttctg gctcttggtc gccgttcacg agacgaccgt
     7921 tgtcgtcgcc gtggtcaccg tgctgcggtg gcgaaaggaa ccccgttcga tgctggcgtg
     7981 gatcctggcc gtccgccttt tgccggtgtt gggaatactg tttttcctgg ttttcggcga
     8041 gccgcgcggc ggctggcacc ggctacggcg aagacgcaga cgcaagcggc tggaagcggc
     8101 tctggcacac aaatggggcg tcttccagca gcagcacccc gccagtccgt tgtccatcac
     8161 cgatcctgtt gtcaaggagt tcgcgcgact gacgaagcgt ctcggggggc atattgccgc
     8221 tccgggcaac gcggtccggg tcttcaatga tgccgaggag acttatgcgg caatcgagaa
     8281 ggcgattgac gaggctcagt cgcacgttca catggaatac tacatctttc agccggacaa
     8341 gaccggccag gcgattcgcg accggttgat tcgcaaggcc cgcgagggcg tgcgctgccg
     8401 cttgcttctg gactacatcg ggtgctggaa cctgtcgcgg cggttcgttc gaccgatgcg
     8461 cgaggcgggt gtggaggtgg cgtatgccat gccggtgatt ccctggcatg ggcggtggcg
     8521 ggtcaactat cgcaatcacc gcaagatcgt cgttgttgac gggcggatcg gattcacggg
     8581 aagccagaac atcggtgatg agtaccgggg ccgcctggcc cgatacggtc cctggcgtga
     8641 cacccatctg atgattcagg ggccttgtgt tcatcatctt caggaggttt tcattgagga
     8701 ctggcattac acgacgcgag aggatctggt gaaggacgaa tacttcccgc tgccgggtcc
     8761 ggccggtgag cacatggttc aactgcttcc ctccggcccc ggccagccta tccgggtgat
     8821 gcaccatctg ctgttcgccg cagtgagcgc tgcgagatcg tccgtctcgg tgattacgcc
     8881 ttatttcgtg cccgacgcgg cgatggttct ggcgctgcaa tcggcttgct accggggcgt
     8941 gcgtgtgcgg ctgctgattc ccaccacgac ggatcatcat ttcgtcctct ttgcaggcag
     9001 aagcttctat ccggagctga tggaggccgg cgtcgaaatc ttcgagcacg atgcgacgat
     9061 gctgcactcg aaggtgatga tcatcgatcg cgcctttgct ctggtggggt cggccaacat
     9121 ggacgaacgt agcttccgtc tgaacttcga attgaccgtc atgctttaca gcccgaccgt
     9181 ggccgatgat ctttacaagg acttcgagac gctaaccgcc cgttcccggc ggattcgttc
     9241 tcgtcatttc gggaagcatt cgtttgcgcg agagatggca accggtctgg ctcgactcgc
     9301 ctcgccgttg ctgtgatcaa tttccgagcg gctcggctca tacaccggcg cctcgtcgta
     9361 tgtgggaaaa ggaacaggcg gcgtgtcgcg gcgacgggaa gctatcggtt gtggggaacc
     9421 ggctgccgtg atcgtgctct cggtggtcag gcgtcagctt tcagcgagag gcggccggct
     9481 tcgtcttgtc tgatctcgcc acggcgaacg agagcgggca gaccgaggcg gtcttcatcc
     9541 ttcatccgtc tttgcctctt gtccgggctc atacgcgcag aacggctctt cggcgaggta
     9601 gtcggcgaat tcgtaaaagg ccctcgcgcg gcagccgccg cagacgttcc tgaacgggca
     9661 gattccgcac ttgccggtca atcggctgaa gtcgcgcagt gcgtgaaaca gttcgctgtt
     9721 gcgccagatg gccccgaagc tcgtccggcg gacgtcgccg gcttccagcg gcaggtagcc
     9781 gcatgggaac actttgcctt cgtgcgaaac gaagcagacg ccgatgccgg ccaggcagcc
     9841 tcgcgtcatg gtgtgaagcg cgctttgccg tgtttgccct gaacgcggcc ggtgttgctc
     9901 gcgggcctgt tggcggatga cgcggtagta gtggggggca caggtcggct tgatttggag
     9961 ggaggttgtc tgctgcaggt tatcaatgag tcgaaggcgg tcctccacct ccttggcgtc
    10021 gagcatctgg tcgtcggcga tttgttctcc gcagccgacg ggcacgagga gaaaatagtg
    10081 cacggccgcc gcgccggctt gctctgccag gcgtaacacg gcctcgatct ggtccttgtc
    10141 gtggcgggcg acggtgcaat tgatctgggt ctgcacgccc agggttcgca gatgtgccag
    10201 ggcggcgatg gcgcggtcaa aggatccggt ctgcatgcgg aagcggtcgt gggtgtcggc
    10261 gtcggctccg tcaagcgaga tgctgacgcg gtcgaagcct gattgggcga ttgttcgtgc
    10321 catgggttcg tcgatgagtg ttccgttgct ggccaaggcg atcatcagtc ctcgattcct
    10381 ggcatgggtg gccaactcga agatgtccgg ccgcatcaag ggctcgccgc cggagaagat
    10441 caacaccggc cggcccgtct cggcgatttg atcgatcagt cgcttgcctt cggtgggggt
    10501 caggtcggtt ctcatcaatt cgcgggagac atcgagccgg cggcagtggg agcattccag
    10561 attgcatccg gccgtggttt cccagaagac caatcggagc gggtgagctt ggtcgctgcc
    10621 ggactcggga atcgggatgg gaccgggatg gggcacgggc actccagaat aacacgctga
    10681 gtctcaggtt cccggggcat tatagcattc atctcgtcgc ggcctatgca gggccgcgag
    10741 gcccgagtcg tacctatcca tttgaaaagc cgctgtctgg ggggtacaac ctcgcaacgt
    10801 cgagcggccg cgctatgggt gaggggggtt cacgagccgc gaggatggag caggatatgt
    10861 cgattgcgga cagtttccgg ctggtatgga cgccgtttct taaaaagcag agcggaacga
    10921 tgatgcaggc cgtcggcctg gtgtgcccgc cggtgctggg gtctggcgga gaactgggac
    10981 tccgggtcgg cgactggcaa caggcggcgc gcaagattga tctgatctcg tatcccgagc
    11041 acctgcacac gcttgacaag ccgcggacgt acctctggct ggttccgcgg ccgagacgcc
    11101 ctgtggcgat cacggccttg tggcggacct cgcttggcaa gtcgtatcgg gcgtcgttgt
    11161 tgttgcatcc ggccgaaccg cgtgagatat gtgtggtttt caagacccac attgatcttg
    11221 gttacacagc gacgtttcag gaggttctgc gcctctatcg tacgcgtctg ctggaacagc
    11281 tcctggagaa ccttgatcgg acggcgaatc gcaaagcggg gcggcgtttc atctggatgc
    11341 tgtccacttg gctgctggag cagtgtctcg atccccgccg tacccgccgc gagtacatcc
    11401 gcaggctgga gcattacatc cgggccggtc aggtggtgtg gggtctgatg cctttcacga
    11461 cgcacagcga gttcttcggc atcgaggaga tgtgtcggag catctatgcg gctcggcggc
    11521 tggccgagcg gtacggcgtg cccgtgccgc aggccgcgaa gatgaccgat gtacccgctc
    11581 acacgtcggc gctggccatg gcttttgccg ccgccggcgg tcgcttcttc cagattggca
    11641 ccaaccccga cagccgcccg ccggaagtgc cgccgctgtt ctggtgggcg ctgccggacg
    11701 ggcaaaggct gctgtgtcac tatcacgcca cctacggtac gcccctgctg ccgccgcaag
    11761 actggccgtg gaagaactgg ctgtccgtcc agatgaccag cgataacgtg gggccgcaga
    11821 atcttgacct tctcgatcat atggactgga tcgagcagca cttcgattgg ccggtgtgtc
    11881 gcacggggag actggaagac ttcgccaccg cggtggagca cgaccacggc gatgagctgc
    11941 ccgtgttcgg gaaggagctg accgactggt ggatctacgg tatcgccagt caagcgggac
    12001 cgacggcact ggcaaagcga accaaggagc gtctggctgc ggtggagacg ctcgcgactg
    12061 cggcagcaat gtcgcagagc aaggacatgg acgccggtct cagaagcctg tttgttcagg
    12121 cttatgagaa gctggcgttg tacacggagc acacctgggg g
//
LOCUS       DAOTPW010000174        12079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5034_length_12079_cov_6.234032, whole genome shotgun sequence.
ACCESSION   DAOTPW010000174 DAOTPW010000000
VERSION     DAOTPW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12079
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_12079_cov_6.234032"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="P5316_15060"
     CDS             <1..499
                     /locus_tag="P5316_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR86325.1"
                     /translation="GLIASGLRGTEAADPELAACCVLTTGTDQIVVRVDGAAAFEPFG
                     AQLMPKTRQLLGRIAEALADGQTHLEIRGHAADGPFPPQAGFRDAMDLSYARARAVTD
                     LLTESGIASDRVSITARAGQTPPLMEDQDNRPVSGRGIEIIVHAREPGVHAFNIAEKE
                     RAENG"
     gene            492..1037
                     /locus_tag="P5316_15065"
     CDS             492..1037
                     /locus_tag="P5316_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86326.1"
                     /translation="MGDQADVQAEAVASTKSAGRKKNMIFGGIFLGIMVAEAVGVAFA
                     VRYFSAGPASAEAQDIAAANGLNSAEGQKTPEDVEVEVVTFRGQNSQARQAMMYDVKV
                     VVAVSGDKEADLKAQLERKRAAIADRFMSTIRAADPQVLAEPDCATLRQQFRQILVEV
                     LGNEEFVKKVLIPQFTSYRAD"
     gene            1097..1717
                     /gene="fliN"
                     /locus_tag="P5316_15070"
     CDS             1097..1717
                     /gene="fliN"
                     /locus_tag="P5316_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRR86327.1"
                     /translation="MTATMADNPNDGLLSQDEINAALQAAGIESQMQEQTPSPASSET
                     SPAQEPDTRVDASGRPFDEVAKAMAEAIAAEQAVAKAAPEASAPATASAPETAPTQTP
                     AQPFEIPTLPPGTLPTAPAHGLDLLRDVDLSVKIELGRSRMLVEDVLRLGEGSVVELD
                     KLAGDPVDVLVNDRLVARGEVLVLNDNFCVRINDILSRDVGQEEVA"
     gene            1807..2673
                     /gene="fliO"
                     /locus_tag="P5316_15075"
     CDS             1807..2673
                     /gene="fliO"
                     /locus_tag="P5316_15075"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRR86328.1"
                     /translation="MTGYMLNSGIPAACLSLVLTGWLSAQQIQPFPDARPSLRIPPQN
                     ILEISDEPNEPAAELYSNHAPPATQPVVTTVKAGPAAPVMPQPGLGTRPATTPPEAAN
                     HQSAPIARSEFLERRPTANQRESESRDISRGQHGAGLWSARDLWPLLSVLALILILAW
                     IVRKCLPGRNSLTGQGAIEVLSRIPLSSKQSLVLVRMGRRLLLLGVTNDDINTLCAVE
                     DPDQVAMLVGEVASGRPGSITSQFAREFAEESQVYDKRIVEEEEQSVDTGGQVRNLLN
                     KVRGLASRRNVA"
     gene            2762..3607
                     /gene="fliP"
                     /locus_tag="P5316_15080"
     CDS             2762..3607
                     /gene="fliP"
                     /locus_tag="P5316_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263236.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRR86329.1"
                     /translation="MSGHGCAHLWVTTLAVLVFSTATLGQSTTPMVPPESTPAGQVDN
                     PLQIPDLSRVLPAATTREGMSTTLQILILMTVLSLAPAILIMTTCFTRIMVVLALLRQ
                     AVGTQQLPPGQVLVGLSLFMTFFVMAPTYSKIYHEAVKPWLDQQPGMTQARALTIAEG
                     HMREFMFDQIERVRNEEDIYLFLEYSGRQSIPIDRQLSRQDVPTLALIPAFILSELKT
                     AFVMGFRVYLPFLVIDMVVATVLISMGMLMLPPVMVSLPFKLLLFVLADGWHLVVTSL
                     LVSFS"
     gene            3646..3906
                     /gene="fliQ"
                     /locus_tag="P5316_15085"
     CDS             3646..3906
                     /gene="fliQ"
                     /locus_tag="P5316_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRR86330.1"
                     /translation="MEVGEAVDMGRDALIMVLTISGPVMAIGLLVGLLISLFQSMTQL
                     HEQTLTFVPKIVAMVGVALVLVPWLTDQLLEYATELLGQSPF"
     gene            3931..4716
                     /locus_tag="P5316_15090"
     CDS             3931..4716
                     /locus_tag="P5316_15090"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRR86331.1"
                     /translation="MPLPLFGFYGFLPAFMLVLFRVSGLMLTAPVFSSMALPVQIRAL
                     LAAAISLTIFPMTLSSLPAQLTLAQTLAGMLGELAVGVLLGLAVNLVFMGVELAAEAV
                     GQMSGMRLGEVFNPLLESSAGAMGELYTLVAMMIFLAIRGDHALIRALLDSFQSIPPL
                     GAVPTEGLIALLLDLLNLAFTITIRVGGPMVLALMLALMCFGFLSRTIPQLHLMSVGF
                     PLKVAVCLAVAAMTMVSMEGVLVEGLREAFDAIRIGLGLLPDA"
     gene            4762..5859
                     /gene="flhB"
                     /locus_tag="P5316_15095"
     CDS             4762..5859
                     /gene="flhB"
                     /locus_tag="P5316_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRR86332.1"
                     /translation="MPDSAQERTEAPTQRRREEARKAGQVARSTDLTAAVVLLGTFLM
                     LDWFGRRLFGNLLVAMRVCFEDHEAALTDPQQILHLMAKVFRLLIAAAGPFLAVVVAL
                     SLLASFLQIGYLITFQNLKPSLNRLNPLTGIKRMFNARSAVQLLMGVLKMTCVAGVAY
                     ATLKDRLDVFATAANLPAVAVVGMGAELFFTVSIRLAIVLLVLGIIDFIYQRYRLEKD
                     LRMTKEEVKDEYKRMEGDPKLKSRRRQVQLEMALHRIKSAVPKADVVITNPTELAVAI
                     RYDPNTMNAPRVVAKGADYLARRIREIAVEHRIPIVERKPLARALYYTVEVGQEIPSQ
                     FYKAVAEILAYVYELTGKRYASQAAVAAAVN"
     gene            5919..8030
                     /gene="flhA"
                     /locus_tag="P5316_15100"
     CDS             5919..8030
                     /gene="flhA"
                     /locus_tag="P5316_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRR86333.1"
                     /translation="MADIALLNSRPFKVLERHRGLLVPIGATLLLFVLLVPLPTQVLD
                     FLLVLNLTLSALVFLTILYIQRPLDFSSFPSLLLAMTMVRLTLNTATTRLILTNGNQG
                     MAAAGHVVQTFADFVTAGNLAVGIIIFIILAVIQFVVITKGATRIAEVAARFTLDGMP
                     GKQMAIDADLNAGIIDEAEARRRRAEVTAEADFYGAMDGASKFVRGDAIAGMVITFVN
                     ILGGIYVGMVQFDMPLMECLEVFTKLSIGDGLVAAIPAFITSVAAGMLVTRSNAKSNM
                     GEELLGQLTSRPIALTLAAVFLGFLSLTPLPKLPLLLLGVGCGGLAYVVTATRASAAA
                     MAAAKAKAETKKPERVETLLAVDPMELDVGLGLIRLVDRKQGGDLLDRITNIRRQVAT
                     DVGIVVPPIRVRDHLKLEPNQYTVKIRGVEVARGEIMPGYYLAIDNGTVSIPIAGTAA
                     TEPAFGLPAVWITEGQRAEAEQRNYTVVPASSVLATHLTEIIKRHADELLTREQTHQL
                     LETLKEKSPKAVEDVVPNLIKVGELQRVLQNLLRERVPIRDLETILETVGDWAPRTKD
                     TDILTEYARNALARTICQLYKDKDNVLHVVTLDPKIEDLVNAHLERTDRGTFLSLPPE
                     TQNRLVKAVQERVEQAMTGAGGAVVAVLCSPQIRMWIRRLIEPAMAHVPVLAFNEIVR
                     GVEVRSVGLVVLTDGDANVSG"
     gene            8005..9402
                     /gene="flhF"
                     /locus_tag="P5316_15105"
     CDS             8005..9402
                     /gene="flhF"
                     /locus_tag="P5316_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRR86334.1"
                     /translation="MEMQTFQGNSMAEVLERVKRSLGPDAVILHTRTLRRGGFLGLGA
                     RTIIEITASRDASALNPADRRAMFARRMEHNRGQRSTRLNAASPAIRMPQAEETPVKP
                     SGDADSTVSTQLAAFSSSLRGEMGELREMVRQLLDRSADPSPAPTHDWPQELRDYYTG
                     LLQNDVAEEIAREVIAEARVRLAESGGRSVTAGEAPQDAQNRDRLKTLIPQVMVETIE
                     RMIPPCEPLQWDDSRRTRFVALVGPTGVGKTTTIAKLAAHFKLREGKRVGLITIDTYR
                     IAAVEQIRAYADILNIPLEVVMTPEGMIDAIANLTDCDLVLIDTSGRSQRDVQRLDDL
                     KEFLDLARRVARLGRMPAESTDESSLEVHLVLSCTAHPSQLIEVAEKFSSLGIDRVVF
                     TKLDEALGLGVILNVIRRLNLKLSYLTTGQDVPDDIEVGQRRRIAELILGRRSPSPGS
                     ADAASAPGPVIDHVA"
     gene            9444..10349
                     /locus_tag="P5316_15110"
     CDS             9444..10349
                     /locus_tag="P5316_15110"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRR86335.1"
                     /translation="MFGREHQTRRVDQAHQLRQLMTSAHAAPTPKQGHIIAVTSGKGG
                     VGKTLLAANLSIALAARGHRVALFDMDMGLANADIVLGIEAAWTWRDLLAGRRSLDEV
                     IVQGPGGIALVPGASGVSQMANLSEFERHQLIAAAERAAQIHDLVVFDCGAGISHNVV
                     SVASFADTILVVTTPEPTSVTDAYAMIKALTQEQSRGRQAMSASVGVIVNLAESRREA
                     RDTYERLAGVAARFLHLPVNDYGYVLRDEHVPAAVRQRYPVVLQYPRCSASSCLMVSA
                     GRLSRELGKGEEQQSLFYRVMNMFL"
     gene            10552..10869
                     /locus_tag="P5316_15115"
     CDS             10552..10869
                     /locus_tag="P5316_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86336.1"
                     /translation="MVRLCGAAFGLFAFSITILLGLLAGNPADVILTRALQAMLAFCL
                     LGIGAGWVACRLLDEHALRKHRELFPAEGLAEQNAGKAPAGSQDKAKPPSAGGRQEVA
                     QPR"
     gene            10913..11689
                     /locus_tag="P5316_15120"
     CDS             10913..11689
                     /locus_tag="P5316_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HRR86337.1"
                     /translation="MVEADVDVQQLWKEYKRTGERTLRNALMEFYLPLVRYTAERISA
                     KLPNEVDVEDLVSAGTFGLIDAIEAFDLSRGVKFETYCSPRIRGAILDELRTMDWVPR
                     LVRSRAHKLETATKVLRAELGRLPSNDEIADRLGMDRSEFEKLLGDANAVLLISLSRS
                     YVDQDSDHELQEIDVLADRRTCDPVEGFSERDLRDVVMRTLTEIERLILILYYYEEMT
                     MKEIGATLDLSESRVSQMHSSILARLRGHLRSRGLLESGV"
     gene            11889..>12079
                     /locus_tag="P5316_15125"
     CDS             11889..>12079
                     /locus_tag="P5316_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86338.1"
                     /translation="MSAMPPNIVGSVLQSHIAQHQVSTVREADESQRDSAGRRQASVV
                     DERDTTVGTTDTDTQIHADA"
BASE COUNT         2279 a         3946 c         3524 g         2330 t
ORIGIN      
        1 cgggctgatc gcttccggcc ttcgaggaac cgaagctgca gaccccgaac tggccgcctg
       61 ctgtgtgctg accaccggaa ccgaccagat cgtcgttcgg gtggatggcg ccgccgcttt
      121 cgagccattc ggggcccagt tgatgcctaa aacgcgccaa ctgctcggcc ggatcgccga
      181 ggcgctcgcc gacggacaga cccacctgga aatccgcggt cacgccgcgg atggcccgtt
      241 ccctccccaa gcgggcttcc gtgacgcgat ggatctgtcc tatgcccgcg ccagagcggt
      301 gaccgacctg ctgaccgagt cgggcatcgc ctccgatcgc gtctccatca cggcacgcgc
      361 gggccaaacg ccacccctga tggaagatca agataaccgc ccggtatcgg gacgcggaat
      421 tgagatcatc gtgcacgccc gggaaccagg tgtgcacgcc ttcaatatcg ccgagaaaga
      481 acgagcagaa aatgggtgac caagcagacg ttcaagccga agccgtcgca tcgaccaaat
      541 ccgccggccg caagaagaac atgatcttcg gaggcatctt cctgggtatc atggtcgctg
      601 aggccgtcgg cgtggccttc gcggtcagat acttctccgc cggacctgcc tccgccgagg
      661 ctcaggatat cgccgcggcg aacggactca actccgcgga gggacagaaa acccccgagg
      721 acgtggaagt cgaagtcgtc acttttcgcg gacagaactc ccaggcacga caggccatga
      781 tgtatgacgt gaaggtcgtc gtcgccgtca gcggcgacaa agaggccgat ctcaaggccc
      841 agctcgagag aaaaagggct gcgatcgcgg atcggttcat gagcacaatt cgggccgccg
      901 acccccaggt gctggccgaa cccgactgtg ccaccctgcg acagcagttt cgccagatcc
      961 tggtcgaagt cctgggtaat gaagaattcg tcaagaaagt cttgatacca cagtttacct
     1021 cgtaccgggc agactagttc gagttcattg attcggtcgc agggcaacgg acggcagaca
     1081 gacaacgaaa aacggagtga cggcgaccat ggcggataac cccaacgatg gactcctgag
     1141 ccaggatgag atcaacgccg cgctgcaagc ggcgggcatc gaatcgcaaa tgcaagagca
     1201 gacgccctcg cctgcatcat ccgaaacttc ccccgctcag gagccggata cccgcgtcga
     1261 tgccagcggc cggcccttcg acgaggtggc caaggcgatg gccgaggcta ttgccgccga
     1321 acaggcagtc gccaaggcgg cacccgaggc gtccgccccg gcgaccgcgt ccgccccgga
     1381 gaccgcgccc acacagacgc ccgctcagcc gttcgaaata ccgaccctgc ccccgggaac
     1441 cctcccgacc gccccagccc atggcctgga tttgctccgg gacgtcgatc tttcggtcaa
     1501 aatcgagctc ggacgcagca ggatgttggt cgaggatgtg ctcaggttgg gcgaaggctc
     1561 cgtcgttgaa ctggacaaac tggccggcga ccctgtggac gtgttggtta acgatcggct
     1621 ggtcgctcgg ggcgaagtgc ttgtgctcaa cgacaacttt tgtgtccgaa tcaacgacat
     1681 cctttcgcgg gacgtcggcc aggaggaggt ggcctgagca aggcgccacg cagccttgac
     1741 aggtgtaccg cagcaagacg cggagtcatc agacaggatg tccgcaggac caggagggtc
     1801 caatcgatga ccggttacat gctcaattcc ggtattccgg ctgcgtgtct ttcgctggtg
     1861 ctcaccggct ggctttccgc ccagcagatc cagcccttcc ccgatgcccg gccgagcctg
     1921 cggatcccgc cgcagaatat cctcgaaatc tccgacgagc cgaatgagcc ggccgccgag
     1981 ctttactcga accatgcccc gccggccact cagcccgtgg tcaccaccgt caaagccggt
     2041 cccgctgctc ctgtgatgcc gcagcccggc ctcggcacca ggcccgccac gacgccgccg
     2101 gaagcggcga atcatcagtc cgctcccatc gctcgcagcg aattcctcga gcgccgaccg
     2161 acggccaacc agcgagagag cgaatcccga gacatcagtc gcgggcaaca cggcgccggc
     2221 ctctggtcgg ccagagatct ctggccgctc ttgtccgtcc tggccttgat cctcatcctg
     2281 gcatggatcg tgaggaaatg cctgcccggc cgcaattcgc tgaccggcca gggggcgatc
     2341 gaggtccttt cccgtatccc tttgtcgagc aagcagagcc tggtcctggt ccgcatgggg
     2401 cgacggctgt tgctcctggg cgtgaccaat gacgacatca acacactctg tgcggtcgag
     2461 gatcccgacc aggtcgcgat gctcgtgggc gaggtcgcca gcggacggcc aggatccatc
     2521 accagtcagt ttgcccggga attcgcagag gaatcccagg tatatgataa acggattgtg
     2581 gaggaggaag agcagagcgt cgacaccggc gggcaggtgc gcaacctgct gaacaaggtc
     2641 cggggtctgg cctccagacg aaacgtggcg tgatgcgggg ccgttgtgcc cggcgatggc
     2701 ccataccctc gcccggcacc gcccggcggc atcagccctg atctgcggcg gagttcgcaa
     2761 catgagcggg cacggatgcg ctcacctctg ggttacaaca ctcgccgtcc tggtgttctc
     2821 gaccgccacc cttgggcagt cgaccacgcc catggttccg ccggaatcaa cccctgccgg
     2881 tcaggtggac aatcccctgc agattcccga cctcagccgc gtcctcccgg ccgccaccac
     2941 ccgtgagggc atgagcacca cccttcagat cctcatcctg atgaccgtgc tcagcctcgc
     3001 cccagccatc ctcatcatga ccacctgctt cacgcggatc atggtcgtcc tggccctcct
     3061 gcgccaggcc gtcggcacgc agcagcttcc gcccggtcag gtgctcgtcg gtctctccct
     3121 cttcatgacc ttcttcgtca tggcaccaac ctattcaaag atctaccacg aagccgtcaa
     3181 accgtggctc gatcagcagc ccggcatgac ccaggcccgg gccctcacca tcgccgaggg
     3241 gcacatgcgg gaattcatgt tcgaccagat cgagcgcgtc agaaatgagg aggatatcta
     3301 tctctttctc gaatactccg gtcggcagag cattcccatc gaccggcagc tcagccgcca
     3361 ggatgtccct acgctggcct tgatccccgc gttcatcctc agcgagctga aaaccgcgtt
     3421 cgtcatgggt tttcgtgtct acctgccgtt cttggtcatt gacatggtgg tcgccaccgt
     3481 gctcatctcc atgggcatgc tcatgctgcc gcccgtcatg gtttctctgc cgttcaagtt
     3541 gctgttgttc gtcctggccg acggctggca cttggtcgtg acatcgttgt tggtgagttt
     3601 ctcgtaaaac cgccggattg atccggtcaa cagggaggtc aaaaagtgga agtcggcgaa
     3661 gcggtggaca tggggcggga cgccttgatc atggtgttga ccatctcagg ccccgtcatg
     3721 gcgatcggcc tgctggtcgg tctgctcata tccctgtttc agtcgatgac ccagcttcat
     3781 gaacagacgc tcacgttcgt gccgaagatc gtggctatgg tcggtgtggc gctggttctg
     3841 gtcccctggc tgaccgacca gttgcttgaa tatgcgaccg agctgctggg acagtcgccg
     3901 ttttgacgag cgagataatg gggctcccgt atgccgttgc cgttgttcgg attctatggc
     3961 tttctgccgg cgttcatgct ggtgttgttc cgcgtcagcg gcctcatgct gaccgcgccc
     4021 gtattcagca gcatggccct cccggttcag atccgggccc tgctggcggc ggccatatcc
     4081 ctgaccatct tcccgatgac gctttcttcc ctgcccgctc agttgacgct cgcccagacc
     4141 ctcgcgggaa tgctgggcga actggccgtc ggcgtcttgc tggggttggc cgtgaacctc
     4201 gtcttcatgg gcgtggaact cgccgccgag gccgtcgggc agatgtccgg catgagactc
     4261 ggcgaagtgt tcaacccgct gctcgaatca tccgccggcg caatgggcga actgtacacc
     4321 ctggtggcca tgatgatctt ccttgccatt cgcggcgatc acgccctgat acgcgccttg
     4381 ctcgacagct tccagtctat ccctccgctg ggcgccgtcc cgaccgaggg cttgattgcc
     4441 ctgctgctcg atctgctcaa cctggctttc accatcacta ttcgtgtcgg cggacccatg
     4501 gtcctggcac tcatgctggc actgatgtgt tttggttttc tgtcacgcac catcccccag
     4561 ctccatctca tgagcgttgg tttcccgctc aaggtcgccg tctgcctcgc tgtggccgcc
     4621 atgacgatgg tctccatgga gggcgtgctg gtggagggtt tgagagaggc ttttgacgcg
     4681 atccggatcg gcctggggtt gctccccgac gcctgatcgt gctgatacga gaggacgtcc
     4741 gcctcacggc gggcgaatgt catgcccgac tcagcgcaag aaagaaccga agccccgacc
     4801 caacgccgcc gcgaggaggc ccgcaaggcg gggcaggtcg ccaggagcac cgacctgacg
     4861 gccgcggtcg tattgctggg cacattcctc atgctcgact ggttcggacg caggctgttc
     4921 ggaaacctcc tcgtggcaat gcgcgtgtgc ttcgaggatc atgaagccgc cttgaccgat
     4981 ccgcagcaga tcctccacct gatggccaag gtgttcagac tgctcatcgc cgccgccgga
     5041 cccttcctcg ccgtggtcgt ggccctttcc ctgctcgcct cgttcctcca gatcggatac
     5101 ctgattactt tccagaacct taaaccgtcg ttgaaccgac tcaatcctct caccggcatc
     5161 aaacgcatgt tcaacgcccg ttcggccgtc cagttgctga tgggagtcct caagatgacc
     5221 tgcgtggccg gcgtcgcata cgcaaccctc aaagaccgtc tcgacgtctt cgccacggcc
     5281 gccaatctcc cggccgtggc cgtcgtcggc atgggcgcag aactcttctt taccgtctcg
     5341 attcgcctcg ccatcgtcct gctggtcctg ggaatcatcg atttcatcta ccagcgatac
     5401 agactcgaaa aggacctgag gatgaccaag gaagaggtca aggatgagta caagagaatg
     5461 gaaggagacc cgaaactcaa gagcagaaga cggcaggtcc agttggaaat ggccctgcac
     5521 cgcatcaagt cggccgtgcc caaggccgat gtggtcatca ccaatccgac cgaactcgcc
     5581 gtcgccatcc gctacgatcc gaacaccatg aacgccccac gcgtcgtcgc caagggagcg
     5641 gattacctcg ccaggcgtat tcgcgaaatc gccgtcgaac accgcatccc catcgtggag
     5701 cgtaagccgc tcgcccgcgc gctgtactac accgtcgagg tcggacagga aataccgtcg
     5761 cagttctaca aggccgtggc cgagatcctg gcctatgtgt acgagttgac cggcaagagg
     5821 tacgcaagtc aggccgccgt ggcggcggcc gtgaattgaa cgggaggatg cgtcacatcc
     5881 tcccgcagag gactgaatac tgaagaatgc gagcttgaat ggccgatatc gccctgctta
     5941 actcccgccc cttcaaagtg ctcgagcgcc atcggggcct gctcgtcccc atcggtgcaa
     6001 cgctcctgct gttcgtgctg ctggttcccc tgcccacaca ggtcctggat ttcctgctcg
     6061 tcctcaatct cacgctctcg gccctcgtct tcctgaccat cctttacatc caacgccccc
     6121 tcgatttctc ctcgttcccc tcgttgttgc tggcgatgac gatggtacgc ctcacgctga
     6181 acaccgcaac cacccgcttg atcctcacca acggaaatca gggcatggct gcggccggac
     6241 acgtggtgca gacctttgcg gatttcgtca ccgccggcaa tctggcggtc ggaattatca
     6301 tctttatcat cctggcggtc attcagttcg tcgtgattac caagggcgcg acacgcattg
     6361 cggaagtcgc cgcccgcttc accttggatg gaatgcccgg caagcagatg gcgatcgatg
     6421 ccgacctcaa cgccggcatc atcgatgagg ccgaggccag acgccgccgc gccgaggtga
     6481 ccgccgaggc cgatttctac ggcgccatgg acggtgcgtc caagttcgtc cgcggcgacg
     6541 ccatcgccgg catggtgatt accttcgtca atatcctggg cggaatctat gtcggcatgg
     6601 tccagtttga catgcccctg atggaatgcc ttgaggtctt caccaagctg agtatcggcg
     6661 acggcctcgt cgccgccatc ccggcgttca tcacctcggt ggccgccggt atgctcgtga
     6721 cccgaagcaa cgccaagagc aacatgggcg aggaactcct gggacaactc acctcccggc
     6781 cgatcgccct gacactggcc gccgtcttcc tgggcttcct gtcgctcaca cccctgccca
     6841 aactcccgct tctcttgttg ggcgtcggtt gcggcggtct ggcctatgtc gtcaccgcca
     6901 cccgtgcgtc cgccgccgcc atggccgccg ccaaggccaa agccgaaacc aagaagcctg
     6961 agcgggtgga aacccttctg gccgtcgatc cgatggaact cgatgtcgga ctgggtttga
     7021 tccgcctcgt cgacagaaaa cagggcggcg atctcctcga ccgcatcacc aacatccgcc
     7081 gccaggtcgc caccgacgtc ggtatcgtcg tgccgcccat ccgcgtccgg gatcatctca
     7141 aactggaacc caatcagtac acggtcaaaa tccgcggcgt cgaggtcgcc cgcggcgaga
     7201 tcatgccggg ctactacctc gccatcgaca acggcaccgt gagtataccc atcgcgggaa
     7261 cggcagccac cgagccggcg ttcggactcc cagccgtttg gatcaccgaa ggacagcgcg
     7321 ccgaggccga acaacgcaac tataccgtcg tcccggcctc cagcgtgctt gcaactcacc
     7381 tgaccgagat catcaagcga cacgccgatg aattgcttac ccgcgaacaa acgcatcagt
     7441 tgctcgaaac gctcaaggaa aagtccccca aggccgtcga ggatgtcgtc cccaatctca
     7501 tcaaggtcgg agagttgcag agagtcttgc agaatctctt gcgcgagcga gtcccgatcc
     7561 gagacctgga aaccatcctc gaaaccgtcg gtgactgggc accccgcacc aaggataccg
     7621 acatccttac cgagtacgcc cgtaacgccc tggcaagaac tatttgccaa ctgtacaaag
     7681 ataaagacaa tgtccttcac gtggtgacgc tggatccgaa gattgaggat ctggtcaacg
     7741 cgcatctgga gcgaacggat cgtggcacct tcctgagcct cccgcccgag acgcagaatc
     7801 gtctggtgaa ggccgttcag gaacgggtcg aacaagccat gacaggtgcc ggcggcgccg
     7861 tcgtcgccgt actctgttca ccccagattc gaatgtggat tcgtcgcctg atcgagccgg
     7921 ccatggcgca tgtgccggtg ttggccttca acgagatcgt acgcggtgtc gaggtacggt
     7981 ccgtaggatt ggtggtactg accgatggag atgcaaacgt ttcagggtaa ctcgatggcg
     8041 gaggtcctcg agagggtgaa acgctccctt ggacccgacg cggtgatcct ccacacccgg
     8101 accctccgcc gcggcggctt cctgggcctg ggggcccgaa cgatcatcga aatcacggcc
     8161 agtcgtgatg cgtccgcctt gaatccggcc gatcggcgtg ctatgttcgc acgtcgcatg
     8221 gagcacaata gagggcagcg gagcacccgg ctcaatgccg catctccggc aatccggatg
     8281 cctcaggcgg aagaaacccc tgtgaagccg tcaggcgacg ccgattccac tgtatcgact
     8341 cagctcgccg ccttcagctc ttcccttcgc ggcgagatgg gcgaactgcg cgagatggtt
     8401 cgccaacttc tcgatcgctc tgccgatcca tcacccgccc caacccacga ttggcctcag
     8461 gaactgcgcg attactacac cggtctgctt cagaacgacg tcgccgagga gatcgctcgt
     8521 gaggtgatcg ccgaggcccg ggtccgattg gccgagtccg gcggtcgatc ggtgacggct
     8581 ggcgaagccc ctcaggacgc ccagaaccgc gatcggctca agacgctgat cccccaggtc
     8641 atggtcgaga ccatcgagcg aatgattccc ccgtgtgaac cgctccagtg ggatgactcg
     8701 cgaagaaccc gctttgtcgc cctcgtcggt cccaccggag tcggcaagac gacgaccatc
     8761 gccaagctgg ccgcccattt caagcttcgt gagggcaaac gcgtcggcct gatcactatc
     8821 gatacctatc gaatcgccgc tgttgagcag attcgggcct acgccgatat cctcaacatt
     8881 cccctggaag tcgtcatgac ccccgaggga atgatcgacg ccatcgccaa tctgaccgac
     8941 tgcgatctgg tcctgatcga tacctcgggt cgaagccagc gggacgttca acgcctcgat
     9001 gacctgaagg aattcctcga cctcgctcgc cgggttgccc gcctcggccg catgccggcg
     9061 gaatcgaccg atgagtcctc gctcgaggtc catctcgtcc tctcctgtac cgcgcatccg
     9121 tcccagttga tcgaggtggc ggagaagttc tcttccctcg gtatcgatcg cgtcgtgttc
     9181 accaagctcg acgaggcgct ggggctcggc gtgattctca atgtcattcg ccggctcaat
     9241 ctgaaattgt cgtatctgac aaccggtcag gatgtgccgg acgatatcga ggtcggtcag
     9301 cgtcgtcgaa tagcagaatt gattctgggc aggcgcagtc cctcgcccgg atcggccgac
     9361 gccgcgtcgg cgcccgggcc ggtgatcgat cacgtcgcgt gagcgatctc ttttcggcgg
     9421 gagaaggtgg ccgggagggc cgcatgttcg gtcgtgagca ccagaccagg agggttgatc
     9481 aggcccatca gctccgacaa ctgatgacct ctgctcacgc tgccccaacg cccaagcagg
     9541 gtcacatcat cgccgtgacc agcgggaagg gaggcgtcgg caaaacgctt ctggcggcaa
     9601 acctgtccat cgccttggcc gctcgcggac atcgtgttgc gctttttgac atggatatgg
     9661 gcttggccaa cgccgacatc gttttgggca ttgaggctgc ctggacctgg cgagacctcc
     9721 tggctggccg tcggtcgctc gatgaggtca tcgtccaggg acccggtgga atcgcccttg
     9781 tcccaggagc gtccggcgtc tcacaaatgg ccaatctctc tgaatttgaa cgacatcaac
     9841 tgatcgctgc cgccgaacgg gccgcccaga ttcatgactt ggtcgtcttt gactgcggcg
     9901 ctggcatttc gcacaacgtc gtttctgtcg cttccttcgc tgataccata ctcgtagtta
     9961 ctaccccgga acccacctcg gtcactgacg cctatgcgat gatcaaagcg cttactcagg
    10021 aacaaagccg cggccgacag gccatgtccg cctcagtcgg cgtgatcgtc aatctcgccg
    10081 agagtcgccg cgaagcccgt gatacctacg aacgtctggc cggcgtggcg gcccggttcc
    10141 tccacctgcc ggtcaacgat tacggctatg tcctccgcga cgagcacgtg cccgccgccg
    10201 tgcggcaacg atatcctgtg gttctgcaat acccgcgctg ttcggccagc agttgcctga
    10261 tggtctccgc cggacggctg tcgcgggaac tgggcaaggg tgaagagcag cagagcctgt
    10321 tttatcgggt catgaatatg ttcttatagg ctcgtggatc gtagacgtta tctcgtcgat
    10381 aacgccgttt tctgctaaac cacccgagag ggtttgccgt tactttctct cataaccttc
    10441 gggccggaga tccgcatggt ttgccggtgt ctttccggac cgcaaggctc gtatgttgcg
    10501 gcccgcatga aggcggttcg cttgagactt cggcaacaag gatgttgcga catggtgagg
    10561 ctttgcggag ctgcgttcgg cttattcgcg ttcagcatca cgattctgct cgggctgctc
    10621 gcgggtaacc cggctgatgt cattctgacc cgggccttgc aggccatgct ggccttctgc
    10681 ctgctcggaa taggtgctgg atgggtcgct tgccggttgt tggacgaaca cgcgctccgc
    10741 aagcatcgcg agttgttccc cgccgaaggc ttggcggagc agaacgccgg aaaggctccg
    10801 gccggctccc aagacaaggc gaagccgccg tccgcagggg gcaggcagga agtcgcccag
    10861 ccccgctgat gcctgacggt cggctattaa ggaccgttct gggaggtagc ggatggttga
    10921 agcggatgtg gatgtccagc aactctggaa ggaatacaag aggacgggag aaaggacgct
    10981 tcgaaatgct ctcatggaat tctacctgcc gctcgtcagg tacaccgccg aacgtatctc
    11041 tgccaagctc cccaacgaag tcgacgtcga ggatctcgtc tccgccggta ccttcggctt
    11101 gatcgatgct attgaggcct ttgatctcag ccgcggagtc aaattcgaaa cctactgctc
    11161 gccccgcatc cgcggcgcca tccttgatga actccggacc atggattggg tgcctcgctt
    11221 ggtcagaagc cgggcacata agctcgaaac cgccaccaag gtcttgcggg ccgagctcgg
    11281 acgcttgccc tccaacgatg agatcgccga ccggctggga atggaccgat ccgagttcga
    11341 gaagctcctc ggcgatgcca acgccgtctt gctgatttcc ctgtcacgca gctatgtcga
    11401 tcaggactcc gatcacgaac ttcaggaaat cgatgtcctc gcggaccgcc gcacctgcga
    11461 tcccgtggag gggttttccg aacgagatct ccgcgacgtg gtcatgagaa ccctcaccga
    11521 aatcgaacgg ctcatcctca ttctctacta ctatgaggaa atgaccatga aggaaatcgg
    11581 agccaccctg gacctgtcgg aaagccgtgt cagccagatg cactccagca tcttggcccg
    11641 cctccgcggc caccttcgca gccgcggctt gctggaaagc ggcgtctgac ctttcgcctg
    11701 atcctcaaaa aggccttttc ggacggccgg caagcacacc gtggcgcaat cctgtcttgt
    11761 ttctggacac gccggacctt cgctcaccag gcgcatcctc tggctgtcaa gaagcttgcg
    11821 gccagagtcc gatataataa ttgccggttc cgtctgcccg gggccgcggc cggcggagga
    11881 caacaaccgt gtccgctatg cctcccaaca tcgtcggttc cgtgttgcag agccacatcg
    11941 ctcagcatca ggtctccacg gtccgggagg cggacgaaag ccagcgcgac agcgccggcc
    12001 gccgacaagc ctccgtggtc gacgagaggg ataccactgt cgggaccacc gataccgata
    12061 cccagatcca cgcggacgc
//
LOCUS       DAOTPW010000175        11904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5121_length_11904_cov_4.864546, whole genome shotgun sequence.
ACCESSION   DAOTPW010000175 DAOTPW010000000
VERSION     DAOTPW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5121_length_11904_cov_4.864546"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="P5316_15130"
     CDS             complement(<1..182)
                     /locus_tag="P5316_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86339.1"
                     /translation="MGKTWPASSVTNTCCAAAPLVLAVACGAWALWGLAKARFPLQLT
                     LPISMVMVVAVWQGLTR"
     gene            complement(573..1199)
                     /locus_tag="P5316_15135"
     CDS             complement(573..1199)
                     /locus_tag="P5316_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86340.1"
                     /translation="MFHDIGHEAFMLGVSGLLVLAVAWLIRRDRVNLCPASLILPFLF
                     LAILALDGGLFRLLRIVGLVARMGEAWPPIGPWQAWYRAVSLVLAVVFAAWALWVLIR
                     ARFPLRLTVATSLVAAVALVRGLESVRWVKAWRVAGVFTSHPLDGPWMAIWAAAPGVF
                     VVTAVVLVLWTSRRKNGSEARPVLGDRCPEEGITQLPHHDRPTEGANG"
     gene            complement(1230..3443)
                     /locus_tag="P5316_15140"
     CDS             complement(1230..3443)
                     /locus_tag="P5316_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86341.1"
                     /translation="MSRFTKRHMSVLSILCVGLVLNLDLARVRAQAPPQEYVLKIERI
                     ETESTVMMGQSFRENDLLMSFLPAARPLFPCRVTKMQIWWQRPAGQYRYPVKVEPLLY
                     DADGWLRYDSDIAVLSPGGLNQVPLARTWWYDENPENRFLTPAIFDEPPVSFQTGIYG
                     GFRIDGEVDRLMSENSANIVCSTEGPWELFNILWRNGSWYLLEDEGLPGNLGIRLVVE
                     PLCPAKIGDMPVWNGSEWEWDGDIDQDDRAILQNQINAFNPADPIFDDYRVYMDLDND
                     GDLDADDMAILDAHSTGSGNVVVCPRIEILTPEIGKTTPTGVRPPDETFQVRNSGGCT
                     LDFTVQSTAFWLSVSPAQASSTGQIQDFTLSFDTESLGPGSYPAWLRVDGNAGNSPQW
                     AHVMLTIGTEGDLDRDGDVDTDDVDLFEKCATGPGLSYDPESLPAGCTLVPDGDGIIL
                     ADLDHDGDVDQADFAGLQANYGLPNGMTLASRASAAKGQLTRPVLLVNKSLSRIPLAK
                     TAPEENGLPDVHPASVSVSEPGAVSGVCSPDSRLKAPEFDEQAAKQNRPLGTLGLIFG
                     IVVVVGVLLFAYWLWGLLSPGEFFGNSSFPDSGQEIQRIRAAIDRLNSLGHPDSNRAF
                     AELLGQDKWYNAEIFGGIVLNRQSLDDVSLKASVFKASKTIAIHESLVNPPFWDDNAL
                     KILAVALFAEWQHTDQPGKTENECQTWLEQWLQQVGWDNLNLPFHHGTNEAADDD"
     gene            4316..6931
                     /gene="clpB"
                     /locus_tag="P5316_15145"
     CDS             4316..6931
                     /gene="clpB"
                     /locus_tag="P5316_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRR86342.1"
                     /translation="MRQDQFTVRAAEALQAAQEQAAQAGHAQLEPLHLLLALLNEGAA
                     NGGGVVAPILEKAGVHVGRVRQLVESELSRRPRVSGGQLVPAPEMQRVLAQAHKEADR
                     LKDKFISCEHLLLALADVDSQAKEVLSLAGASREVLLSAMKEVRGHHRVDSENPEDTY
                     QALQRYGRDLVELARRGKIDPVVGRDEEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLALRVVNADVPTVLKNKRIIALDLGAMIAGAKYRGEFEDRLKAVVREVVESDGEVI
                     LFIDEMHTIVGAGRAEGAIDAGNLLKPALARGELRCIGATTLDEYRKHIEKDAALERR
                     FQPLYVSEPTVEDTIAILRGLKPRYDAHHGVRIQDSALVAAATLSHRYIADRFLPDKA
                     IDLVDEAASRLRIENDSMPSELDEIRRKIMQLEIEREALRKEKDKASRKQLEQVEKQL
                     ADLNEQNASLTARWEKEVGMLKQTKSLREQLDAKQTELAEAQRRGDWERAARIQYGEI
                     RELERQIKEQEELMARLREQGGMLVHEEVTAEEIAEVVSKWTGIPVSRMLEGEKEKLL
                     KMEERLARRVVGQEEAIRAVSDAVRRSRAGIGDPNRPVGSFMFLGPTGVGKTELCKAL
                     AEFLFDDEAAFIRIDMSEFMEQHAVARLIGAPPGYVGYEEGGMLTEAVHRRPYCVVLF
                     DEIEKAHRDVFNVLLQVLDDGRLTDGHGRTVDFRNTIIVMTSNIAGAMIQQMAENEAI
                     DVEIESRVREELKKHFRPEFLNRIDETIIFHRLTQEDLARIVDIQLDLLRKRLADRRI
                     SVRLTDAAKAQLARDGYDPVYGARPLKRLIQKEIENPLARRLLAGDFGENQCIEIDSD
                     GEMFRFTTVAPATAN"
     gene            complement(7357..8400)
                     /locus_tag="P5316_15150"
     CDS             complement(7357..8400)
                     /locus_tag="P5316_15150"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HRR86343.1"
                     /translation="MIGLCLWMVIVAAGPADDREDAPSILGRTQLECPACHKSFTTIV
                     CVRSNTRAGVDRDLFARSLGAQPEFYRISTCPHCGYSGYLADFDPSVTLMPDVANRIL
                     RSPGLKLPPGFGPDSDPSELDASVRYALAVTCYQWRQKSDEAIGWLYLRSSWVEREKG
                     AHVPPESRLRRALAYIERWRPALGPTDNQLDVEMKLATRTSEALLTGRFTRYQRPYIE
                     LALALILRRHGENAQAGSILRRLQQADGNLFSEDLRTGMTAMQQSIIREKEHQEQAAN
                     AFERALLAGLVSADNRPAAMYLLGELFRRLSREAEAVTWFDRALKEPNLPANLREWAE
                     QQRSHCLGSRPRQ"
     gene            complement(8565..9530)
                     /locus_tag="P5316_15155"
     CDS             complement(8565..9530)
                     /locus_tag="P5316_15155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86344.1"
                     /translation="MNTPQLKRVTVLAVLLGMSVTSIAAAGIGDDLRYGLQLYGVKFQ
                     KKYDPLSKGYVLNVSFTNPDTGLPYYNNTHFYLGFGDLVMESGGVSLSATYSERLVPA
                     VRFALSTRGSPLDYRYESYLGSEDMVIDGSLLLNIDTYVNQWGFYDITLQASNRATAT
                     SKHGELEDLSARLDYDIGPINLSGNIWLDAAAVLTNPFFERFGTENPFAAFGSSLASL
                     SATGGRIEDFIARLQSGQLLNDDELAQLINNSLLASLLDKQPSSDLLSKLIIPDGLLN
                     GDCPACGEKPQATVQWLAVPEPGMLILLAGAGAGFYLFSCRRGKR"
     gene            complement(9744..11120)
                     /locus_tag="P5316_15160"
     CDS             complement(9744..11120)
                     /locus_tag="P5316_15160"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRR86345.1"
                     /translation="MSSKAATLLFERNKDVWLAELLTFLRFPTISTLSEHVADMGQCA
                     EWIRDRLIESGLQAEVVPTDGHPAVFGDTGPVAGAPTFLVYGHYDVQPVGDLSLWQSP
                     PFEPTIRDGAIYARGSADDKGQVMIHLAALRCWKDTGASWPVRVKLLIEGEEEIGSKH
                     LSTLIENNRERLACDNVLISDTAKLNAETPALTCSTRGLVFKEITVQGPTHDLHSGVY
                     GGAVANPANVLATIIASLHDERRRVAIPGFYDDVATLSDEQRRNLASYGISDADLLAA
                     TGSPTPYGEEGFSTAERCGVRPTLDVNGMVSGFTGEGSATIIPASATAKISMRLVAHQ
                     KPDKISAAFDEAVRRVAPSAVRLKIVTRSTCEAYLCPLDSPGMQMARQALAEGFGREP
                     VLTHEGGTLPILPLFKRALGADSLLMGFAMPNCNLHSPNEFFHVRDFELGVRSILRFI
                     EIAGDSVR"
     gene            complement(11356..11727)
                     /locus_tag="P5316_15165"
     CDS             complement(11356..11727)
                     /locus_tag="P5316_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRR86346.1"
                     /translation="MPESQRLLIETIKDVTVVTFQDISILDALQIDEIGEELYELVEK
                     KDCRQLILDFTNVKFLSSSALSVLITLHKKAAQNKGQVVLCDLRDDLKKVFEITRLDR
                     MFTFCPTLDDALAVFGRTTAG"
BASE COUNT         2435 a         3596 c         3590 g         2283 t
ORIGIN      
        1 cgagtcagtc cctgccacac agcgaccacc attaccatcg agatcggaag cgtaagctga
       61 agcgggaatc tcgccttggc caggccccat agggcccacg caccgcaagc gacggccagc
      121 accaatggcg cggctgcgca gcatgtgttt gttacgctgg aagccggcca agtcttgccc
      181 atccatccgg ccgtgtcgct gagcatcgag tagaatgtga gtagcccccg tccagagaca
      241 gaaatgccaa gagaatgaac gacagaaaca gccccggaaa gcgaagcttg tccccgcgta
      301 tcatgcaaga gattgccagc gcaattagcc ctagcattcc caacaggagc aaccaaccgc
      361 cgatgccgat gtagataaag ctgcgaagca ttcctcgctc ctcgcgctcc cggtgtcagg
      421 ccgggctaat ccctggcagc ctcattcgtg ccatggtgga acggaaggtt gaggttgccc
      481 catcccacct gttgcagcca ctgctcaagc caggtttggc actggccttc ggtattgttc
      541 ggctcatcgg cacgctgcga cgggaccagg cttcagccgt tcgcgccttc agttggccga
      601 tcatggtgtg ggagctgcgt aattccctct tcggggcacc gatcgccgag aacaggacga
      661 gcctcactgc cgttcttccg acgggaggtc cacagaacga gcacgactgc ggtgaccaca
      721 aacacccccg gcgctgccgc ccagatcgcc atccacggtc cgtcaagcgg atggctcgtg
      781 aacacgcccg cgacgcgcca tgccttgacc caacggacgc tctccagccc gcgcaccagg
      841 gctactgccg cgacaagcga ggtcgcaacg gtaagccgaa gcgggaatct tgctctgatc
      901 aaaacccaga gtgcccaagc cgcgaaaacc acagccagca cgagcgacac tgccctgtac
      961 catgcttgcc acgggccgat gggtggccac gcttcaccca ttcgtgccac taagccgacg
     1021 attctgagca gcctgaacag gcccccatcc aaggcaagga tggcaaggaa cagaaaaggc
     1081 agaatcagcg atgcagggca gagattaact ctgtctcggc gtatgagcca ggcgacggcc
     1141 aacacgagca gccctgacac tcccaacatg aacgcttcat gtccgatatc atgaaacatg
     1201 taaccaccct ctaatcttgg acgaagacct taatcgtcat cggctgcttc gtttgtgcca
     1261 tggtggaaag gaaggttcag attatcccat cccacctgtt gcagccactg ctcgagccag
     1321 gtctggcatt cgttctcagt ctttcccggc tgatccgtat gctgccactc ggcaaagagt
     1381 gcaaccgcca gtatcttcag ggcattgtcg tcccaaaacg gcggatttac caggctttca
     1441 tggatggcaa tggtcttcga ggccttgaat accgatgctt tcaagctaac gtcgtcaagc
     1501 gactgtcggt tgaggacgat cccaccaaag atttcggcgt tgtaccactt gtcctggccg
     1561 agcaattcgg cgaacgcccg gttcgaatcc gggtgcccga gagagttgag ccggtctatt
     1621 gccgctcgga tgcgctggat ctcttggccg gaatcgggga atgatgaatt cccaaagaac
     1681 tcccccggac tcaacaaccc ccaaagccag tacgcgaaca acaggacacc gactaccacc
     1741 acaataccaa agataaggcc cagcgtaccc aacggccggt tctgcttcgc ggcctgttcg
     1801 tcgaactccg gcgccttcag tcgggaatcg ggagaacaca cgccggacac ggcccccggc
     1861 tcgctcactg atacagatgc tggatggaca tcgggtaggc cattctcttc tggggcggtt
     1921 ttcgccaatg gtattcttga cagggacttg ttcaccagaa gcacaggcct ggtgagttgg
     1981 cctttggctg cggaggcccg gctcgccaag gtcatcccgt tcggcaagcc gtagttcgcc
     2041 tgaagcccgg caaagtcggc ttgatcgaca tccccgtcgt ggtcgagatc ggcgaggatg
     2101 atcccgtcgc cgtctgggac cagagtgcag ccggcaggga gtgattcggg gtcgtaagac
     2161 aggcccgggc cggtggcgca tttctcgaaa aggtcgacgt catccgtgtc aacgtcccca
     2221 tccctgtcca ggtccccctc ggtcccgatg gtgagcatga cgtgggccca ctgcggtgag
     2281 ttgccggcgt tcccgtcgac gcgcagccac gccggatagc tccccggtcc gagcgattcc
     2341 gtatcaaacg acaaggtgaa gtcctggatc tggcctgtag aactggcttg agcgggcgac
     2401 acgctgagcc agaaagcagt cgactgcacc gtaaaatcaa gggtgcatcc gccggaattc
     2461 cttacctgga aggtctcgtc cggtggccgg acgccggttg gcgtggtctt cccgatctcc
     2521 ggggtgagga tctcgatcct tgggcaaacg accacgtttc ccgagccggt ggaatgggcg
     2581 tcgaggatcg ccatgtcatc ggcatccaga tcgccgtcgt tatccaggtc catgtagacc
     2641 cgataatcgt cgaatatggg atcggccggg ttgaaggcgt tgatttgatt ctggaggatg
     2701 gcgcgatcat cctgatcgat gtctccatcc cactcccact cggaaccgtt ccagaccggc
     2761 atatcgccga tcttggcggg acaaagcggc tcgacgacca agcgaatccc caggttgccg
     2821 ggcaagcctt catcctcaag aagataccac gagccattcc gccagaggat attgaacaat
     2881 tcccaggggc cttctgtcga gcacactata ttggccgagt tctccgacat caatcggtcc
     2941 acctcgccgt cgatccggaa ccccccgtat attccggtct ggaacgagac cggcggctcg
     3001 tcgaagatcg ccggcgtcag aaaccggttt tccggattct cgtcgtacca ccatgtccgg
     3061 gcgagcggca cttggttcag tcctccgggc gacaagaccg cgatgtccga atcataacga
     3121 agccaaccgt cagcgtcata cagcaacggt tcaaccttca ccggatatcg gtactgtcca
     3181 gcaggacgct gccaccagat ctgcattttg gtgacccgac agggaaagag gggccgcgct
     3241 gcggggagga aggacatcag gaggtcgttt tccctgaagc tttgacccat catcacggtg
     3301 ctttccgtct cgatacgctc tatcttcagc acatactctt gcggcggtgc ctgtgcgcga
     3361 acgcgtgcta ggtccagatt cagtaccagt ccaacgcaca ggatactgag caccgacatg
     3421 tgccgcttgg tgaacctact catgaacgac ctcccgggct ttgccctgct aggactccga
     3481 tgctctgggg caggttgccg aaacaaagaa gggaatgcca cccgcccatg ccgccttgga
     3541 gggtcgccaa acccttcgtg ttgacacggg gaaggacgac actcccaagc gggagtgcgt
     3601 ccagtcctcg tgcgcccaac acgactcagg gaattgcccc cttcgaggat ttcgagggca
     3661 agcactcaag tctgaagcgc ggactgtcaa atcaactttt cttggttcga atccgtactc
     3721 ctaacaaagg acctcgtccg aactgcaaca tgatagctta gtagcggcgc cggcagtctt
     3781 cagtatcaac tcggatacac aaggaaccga acttgccgac agttctcgga acgctgtcgt
     3841 cctgacacac aatcttccga agcctccgtg aaaggctggc ctgtttatgg aaggctagtc
     3901 tgtataatga attctatcga ctccaaagat tgaggcaaga ccccacaaga agcatttgtt
     3961 ctgattagca gtcaaatctg tcggtagaat ctttcaaatt attaattaac tccgtttacc
     4021 ttcgaacctg tcagggtcct gatacaaacc cgtgtgtgac gggtctccgc ccgctcagtt
     4081 tccgaggtct tggggccggc tgcagatcct ttcggtccat cggggcgtcg aggatttgcg
     4141 gcactttgaa tgggccttcc cagatgccga acgcctgccc tgccaatttg gcagcaaggg
     4201 ggcttaggcc gtctgaagcc gctttttctg cctcgccaga gccgtttccg cctggcatgc
     4261 gctttgctgg ctgaaccgcg gagcacagtg cgctgttcca ggaaggtttt ctgccatgcg
     4321 tcaagatcag ttcaccgtgc gagcagcaga ggccctgcag gcggcccagg agcaagctgc
     4381 ccaagccggg cacgcgcagc tggagccgct gcatctgttg ctggctctgc tgaacgaggg
     4441 ggctgccaac ggcgggggag tcgtggcccc gattctggag aaggccgggg ttcacgttgg
     4501 gcgggtccgc caactcgtgg aaagcgagct gtcgcggcgg cctcgggtca gcgggggcca
     4561 gctcgtgccc gcgcccgaga tgcagcgagt cctcgcccaa gcgcacaagg aagcagatcg
     4621 actcaaagac aagttcattt cgtgtgaaca cctgctgttg gccctcgccg acgtggattc
     4681 acaagccaaa gaggtgctct cgctggccgg agcgagccgg gaggtgcttc tctcggccat
     4741 gaaagaagtt cgcggacatc accgggtcga cagcgagaac cccgaggaca cgtaccaggc
     4801 cttgcagcgg tatggccgtg acctcgtcga gctggctcgc cggggcaaga ttgacccggt
     4861 ggtcgggcgc gacgaggaaa tccgccggat catgcaggtc ctgtctcgcc ggaccaagaa
     4921 taaccccgtg ctcatcggcg agcccggggt cggcaagacg gccattgtcg aggggttggc
     4981 tctccgcgtc gtcaacgccg acgtccccac ggtactgaag aacaagcgga ttattgccct
     5041 ggacttgggt gcgatgatcg ccggagccaa gtatcgcggc gagttcgagg atcgcctcaa
     5101 ggccgtggtc cgcgaggtgg tcgaaagcga cggcgaggtg attcttttca tcgacgagat
     5161 gcataccatc gtcggggccg gccgcgccga gggagccatt gacgcgggaa acctgctcaa
     5221 gccggcactg gctcgcggcg agctgcgatg catcggtgca accaccctgg acgagtaccg
     5281 caagcacatc gagaaggatg cggccctgga gcgacggttc cagcccttgt acgtgagcga
     5341 gccgacggtc gaggacacca tcgccatttt gcgcgggctc aagccgcgat atgacgccca
     5401 ccacggggtg cggatccaag actcggccct ggtcgcggcg gcgacgctgt cccatcgtta
     5461 cattgccgac cggttcctcc cggacaaagc gatcgacctg gtggatgagg cggcctcgcg
     5521 gttgcggatc gagaacgact cgatgccgtc ggaacttgat gagatccgcc gaaaaatcat
     5581 gcaacttgag atcgagcgcg aggcccttcg caaggagaag gacaaagcct cgcgaaagca
     5641 actcgaacag gtcgaaaagc aactcgcgga tctcaacgag cagaacgcat ccctgaccgc
     5701 acgctgggag aaagaagtcg ggatgctcaa gcagacgaag tcccttcgtg aacaacttga
     5761 tgccaagcag accgagctgg ccgaggctca gcggcgcggt gattgggaac gagcagcgcg
     5821 catccagtac ggcgagattc gggagctgga gcggcagatc aaggaacagg aagaactcat
     5881 ggcccggctg cgcgagcagg gcggcatgct ggttcacgag gaggtcaccg ccgaggagat
     5941 cgccgaggtg gtcagcaagt ggacgggcat tccggtgtcc cggatgctcg aaggcgagaa
     6001 ggaaaagctc ctgaagatgg aagagcggtt ggctcgccgg gtggtagggc aggaagaggc
     6061 gattcgcgcg gtcagcgatg cggtgcggcg aagtcgcgcc ggcatcggcg atccgaaccg
     6121 gccggtgggg tcgttcatgt tcctcgggcc gaccggcgtg ggcaagacgg agttgtgcaa
     6181 ggcgctggcc gagttcttgt tcgacgatga ggcggcgttc atccgcatcg acatgagcga
     6241 gttcatggag caacacgcgg tggccaggct gattggagcc ccgccggggt atgtcggtta
     6301 cgaggaaggc gggatgctca ccgaggcggt ccaccggcgg ccgtattgcg tggtcctctt
     6361 cgacgagatc gagaaagctc accgcgacgt gttcaatgtg ttgttgcagg tgcttgacga
     6421 cgggcggttg accgatggtc acggacggac cgtcgacttc cgcaatacca tcatcgtcat
     6481 gaccagcaat atcgccggag ccatgattca gcagatggcc gagaatgagg ccatcgacgt
     6541 ggagatcgag tctcgggtcc gggaggaact caagaaacac ttccgaccgg agttcctgaa
     6601 ccgcatcgat gagacgatca tcttccaccg gctcacccag gaggatctgg cccggatcgt
     6661 tgacatccag ctggatctgc tgcgaaagcg gctggcggac cgacggatca gcgtgcggct
     6721 gaccgatgcg gccaaggcgc agttggcccg cgacggatat gacccggttt acggtgcccg
     6781 gccgctcaag cgactgatcc agaaggaaat cgaaaacccc ctggcccggc gcttgctggc
     6841 cggcgatttc ggcgaaaacc aatgcatcga aatcgacagc gacggcgaga tgttccgctt
     6901 tacgactgtc gccccggcga cggcgaattg agccggcacg cggcttttct caacgtgatg
     6961 agcgtatgcc gccggcgtct gcggcccggg ttgccgcagg agttgccggc ggggccgaca
     7021 agcataggtc tgtgctccgg cccgatccct gggctgatat gatcgtctga cggcaggttg
     7081 ttttgacacc tgtcgaaatc acatgcgatt cttgtatccg tcccgcagat cgcttctgtc
     7141 gtcctgacca tccgcaggtg ccgaccttat tggtttctta gtacgtgccg gcgcccccgc
     7201 caagcgccac gtccaccgcc ctgcggggac ttgatcctta tccgaccagt gtagggctca
     7261 cacccccgtt ggcccgttgc tgccggttgc cgctataaga aacgagaagg acgcagatga
     7321 gaagcgagct gcacgcattc aacgcgaggc gaggcgtcac tgtcggggcc gggatcccag
     7381 gcagtgagac cgttgttgct ctgcccattc ccggagattg gcgggcaaat tgggttcctt
     7441 cagtgccctg tcaaaccacg tcacggcctc agcctcacgg ctcaaccgcc tgaacagttc
     7501 ccccagcagg tacattgccg ccggccggtt atccgcagac accagaccgg cgagcaacgc
     7561 tcgttcaaag gcgttcgctg cctgctcctg gtgttctttt tcccgaatga tcgactgctg
     7621 catggcggtc atgcctgtcc gaagatcttc cgaaaaaagg ttgccatcgg cctgttgcag
     7681 gcggcggagg atcgatccgg cttgcgcgtt ctcgccgtgc ctccggagga tcaaagccaa
     7741 cgcaagctca atgtagggcc gctggtagcg cgtgaatctg cccgtcaaca gggcctcgct
     7801 tgtgcgcgtg gcaagcttca tctcgacatc caactggtta tcagtcgggc cgagcgcagg
     7861 ccgccatcgc tcgatataag caagggcgcg tctcaaacga gattcaggcg ggacgtgcgc
     7921 ccctttctca cgttccaccc aggatgaccg caaatacagc cacccgatcg cctcatctga
     7981 tttttgtcgc cattgatagc aggttaccgc cagcgcataa cgcacggagg catccaattc
     8041 gctgggatcg ctgtccgggc cgaagccggg cggcagcttc agccccggcg atcgaagaat
     8101 ccggtttgcc acgtcgggca tcaacgtgac gctcggatcg aaatcggcga gatagccgct
     8161 gtagccgcag tgcggacagg tcgagatgcg gtagaattcg ggttgtgccc caagtgacct
     8221 tgcgaacagg tcgcgatcga cgccggcacg ggtattgctg cgaacgcaca caatggtcgt
     8281 gaagctctta tgacaggccg gacattcgag ctgtgtcctt cccaggatcg agggagcgtc
     8341 ctccctgtcg tcagcagggc ccgccgcgac gatgaccatc caaagacaca acccgatcat
     8401 gattcgcctc gcccaatgtg cacattctct cgcggcaaac ccggaacggc aaccaagatg
     8461 cgccggccgc tgcgggcggc tcgatggcag gacggactca taccgactcg gcggaaagtc
     8521 gtgcctgatg gtgatcctgc aggccccgtg acgcgcagaa gggttcagcg tttgccgcga
     8581 cggcacgaaa acagatagaa accggcacca gccccggcca gcaggatcag catgcccggt
     8641 tcgggtaccg ccagccattg caccgttgcc tgcggcttct cgccacaggc cgggcaatca
     8701 ccattcagca acccgtcggg aataatcagt ttggacaaca ggtcgcttga tggctgcttg
     8761 tccagcaggc tggccagcag cgagttgttg atcaactgtg ccaactcgtc gtcattcagc
     8821 aattgaccgg attggaggcg ggcgatgaag tcctcgatgc ggccacccgt cgcgctcagg
     8881 ctggccaggc tgctgccgaa tgccgcgaag gggttctcgg ttccgaaacg ctcgaaaaac
     8941 gggttggtca gaaccgccgc cgcatccaac cagatattcc ctgacaggtt gatggggccg
     9001 atgtcatagt cgagcctggc cgagaggtct tcaagctcgc catgtttgct ggtggcggtc
     9061 gcgcggttgg atgcctgaag cgtgatgtcg tagaaacccc actggttcac ataggtgtcg
     9121 atgttcagca gcaggcttcc gtcgatgacc atgtcctcgg agccaaggta gctttcgtag
     9181 cggtagtcca gcggagagcc tctggttgac aacgcgaatc tgacggccgg cacgagcctt
     9241 tcgctgtatg tggcgctcag ggagacccca ccgctttcca tgaccaggtc gccgaaaccg
     9301 agatagaaat gggtattgtt gtagtagggc aatccggtat caggattggt gaaagacacg
     9361 ttcaacacgt agcccttgct tagcgggtcg tacttcttct ggaacttcac gccgtagagc
     9421 tgaagcccat atcgcagatc atcgccgatt ccagccgcgg ctatgctcgt gacactcatg
     9481 ccgagcagaa cggccagcac agtcacccgt ttcaattgcg gggtattcat cctcgtgtct
     9541 cccgtatccc gggtccggta tcgaagcgga actggaaacg cccggcctgt tgaaaccgcc
     9601 ggcgcaaacc ccgaccgccc agtcaccatt attgtatgtt catcggccgt cctgtggaag
     9661 gtgttcaact caagagtgcc ggcgaaatca gagcttatgc gtcttttcga gctgtcgacg
     9721 cgggactgag gaattgtgct ctgttatcgg acgctgtcgc ccgcgatttc gatgaacctg
     9781 agaatgcttc gcaccccgag ctcgaaatcc cgaacatgga agaactcgtt cggactatgc
     9841 aagttgcagt tcggcatggc gaaacccatc aacaggctgt cagcccccag cgccctcttg
     9901 aagagtggga ggatcggcag cgtgccgccc tcgtgtgtca gcacgggttc gcgcccgaag
     9961 ccctcggcca gagcctgccg agccatctgc atgcccggag aatcaagcgg gcagaggtat
    10021 gcctcgcatg tggaccgagt aacgatcttc aaacgcacgg ccgagggtgc aacccgtcgg
    10081 acagcctcgt cgaaagcggc ggagatcttg tcgggctttt ggtgtgctac cagacgcatg
    10141 gagatcttgg ccgtcgcgct ggccggaatg atggttgccg agccttcgcc cgtgaaaccg
    10201 ctgaccatcc cattgacatc aagcgtggga cgaacgccgc aacgctccgc ggtggagaac
    10261 ccttcctcac cgtagggggt cgggctgcca gtggctgcca gcagatccgc gtccgaaatg
    10321 ccgtacgagg ccaggttgcg ccgctgctca tccgacagcg tcgcaacgtc atcgtagaag
    10381 cccggtatgg cgacccgccg acgctcgtca tgcaatgaag cgatgatcgt cgccagcaca
    10441 ttggccgggt tggccaccgc acccccgtag acaccgctgt gaagatcatg agtcggccct
    10501 tggacggtga tctccttgaa aaccaggccg cgcgtggaac aggtcagggc cggcgtttcg
    10561 gcgttgagct tggccgtgtc ggagatcaga acgttgtcgc aggccagcct ctcgcgattg
    10621 ttctcgatga gcgtcgacag atgctttgag ccgatttcct cctcgccttc gatgagcagc
    10681 ttgacccgca ccggccagga cgccccggta tctttccagc agcgcagagc cgccagatga
    10741 atcatgactt ggcccttgtc gtcggcgctg ccgcgtgcat agatggctcc gtcacgaatg
    10801 gtcggctcaa acggtgggct ttgccagagg ctcaggtccc cgaccggctg gacgtcgtaa
    10861 tggccgtaaa ccaggaacgt cggtgcaccg gcgaccggac cggtgtcgcc gaacaccgcc
    10921 gggtgaccgt cagtcggcac gacctcagct tgcaggccgg actcaatcag tctgtcgcgg
    10981 atccattcag cgcattgccc catatccgcc acgtgttcgg acagcgtgga aatggtcgga
    11041 aaacgcagaa aggtcagcag ctcggctagc cagacgtcct tgttccgctc gaatagcagt
    11101 gttgcagctt tcgaactcat ggcgcttcct cgcttcaagg gtcatcactc gaccgtgggc
    11161 acattatgcc ggatacgcag cccggataat agcccggctg cctggaaggg tacgtccttc
    11221 ctccatggta atggctgggg cgctcgcgtc cgggcaccgg aacctgcaac gccttggtgt
    11281 ctgggcggcc tccatcccgt acgcgctgcg ccgattcccc gacagcggcc tcgtaaggac
    11341 cacgatgagc ggtgcctagc cggcggtggt tcgtccgaag accgccagtg catcgtcaag
    11401 ggtggggcag aatgtgaaca ttcggtcgag acgggtgatt tcaaagacct tcttgaggtc
    11461 gtcgcgcaga tcgcagagca cgacctgccc cttgttctga gcagccttct tgtgcagcgt
    11521 gatgagcacg ctgagagccg acgacgacag aaacttgacg ttcgtgaagt cgagaatcag
    11581 ttgcctgcaa tccttctttt caaccagttc gtacaactcc tcaccgatct cgtcgatctg
    11641 aagagcatcc aggatggaga tgtcctggaa ggtgacgacg gtgacgtcct tgatggtctc
    11701 gatcagtaat cgttgtgatt cgggcatgtc tcttcctgcc actacgggtc cctggttatc
    11761 cgagccgcaa ttctaagcga agaccaggcc cttggcgaga gggcaatcgc cgtttcggag
    11821 gtagtgtcaa cccctgtgta aattgctctg gggcatcgct ccgagtactc tcctcatgct
    11881 gcatgggtct tggttttctt cctc
//
LOCUS       DAOTPW010000176        11756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5198_length_11756_cov_5.110418, whole genome shotgun sequence.
ACCESSION   DAOTPW010000176 DAOTPW010000000
VERSION     DAOTPW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5198_length_11756_cov_5.110418"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="P5316_15170"
     CDS             <1..1291
                     /locus_tag="P5316_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86347.1"
                     /translation="VPGTKTGGNAIYRIDTDGFVTEIFREPVMILGMAEADGTLYAAT
                     GNEGRIYALTPNADRIVMLARLEAAQATCLLRLPDNRLLIGTANAATVVELADRYAAK
                     GTLTSKVLDAEQISAWGRVRWNATVPTGTKLSIATRSSNVEDEESEAWEDWSSEMDAT
                     YPQSILSASARFLQYRVSFETTVPDATPVLRSVEIFRIQPNRAPQITSVEVLAALDEA
                     KKPTASPKVKMAAGASGFSGGEEASGPKYNWVVKWNAGDPNGDPLTFEVFYREPNARR
                     WIRIAKDLSEPLHIWDTRTVPDGKYEVRVLAKDSKANPPGTDLSAARISDMILVDNTP
                     PDVTVDSITPKGKRAVRISFICHDALSRIAGASYNVDSDEKWTPLASDDNIFDEPSET
                     VTFTVDDLDTGEHRIAIRVEDERGNFRYVSRLVTVGD"
     gene            1360..2154
                     /locus_tag="P5316_15175"
     CDS             1360..2154
                     /locus_tag="P5316_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86348.1"
                     /translation="MTNTTHESLVPAPKDDQPPASADTQSPIADGRTRRAARWWCRHP
                     CPPYACPTGARRIFIPVYGWLEVDDTHRARVEHYFHAPMLILALLVLPILLIEWSYGD
                     RINAAHPAARVAICVGGAIIWLAFLVEFIIKVSIAESRWRYVTRNWLDIAIVALPLLR
                     PLRALRVVRAAEVTRVSRLFTIRGVITKLFQTAAATVLGLEFVRRILDRFRPPQADGQ
                     APQYERWSQACLIAEIKRLNARIHELEQMLHHSADRKRAESDKTTP"
     gene            complement(2252..5428)
                     /gene="topA"
                     /locus_tag="P5316_15180"
     CDS             complement(2252..5428)
                     /gene="topA"
                     /locus_tag="P5316_15180"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRR86349.1"
                     /translation="MRAKGAPVATEETKSETSGEQNKAQARVKGRESAGSKALVIVES
                     PAKAKTINKYLGGGYVVKASVGHVRDLPERGLGVEIDRGFAPTYELVPGRRKVVSELK
                     KLAERAPAVYLATDLDREGEAIAWHLAQALDLDESKIRRVIFNEITRSAIQEAFSHPR
                     HIDMDKVNAQQARRILDRIVGYELSPLLWKKVAKGLSAGRVQSVALRLIVEREKEIKD
                     FIPSEYWDIGAYFARDPAEGERLEPMWREFLSGGEKERTLKEMGEWLAQHSAFGATMV
                     ALDGRPFKPTGSYDAAAGADRAFVSAVGQAREAAEALGVRIERQNEVPWPEYEHRGLR
                     KVTLAGRLDRQQAPAFTVTDVQKRRALTKPSPPFTTATLQQAASSHLHLSASRTMRLA
                     QELYEGIELAGEDGPVALITYMRTDSTNLSAESVKAAREWISENCGRRYLPDEPNVYA
                     SGKRAQEAHEAIRPTDVTRTPESLKGHLAAPLQKLYELIWRRFVACQMTPAEWDSTTV
                     LIQAKTVLGEAEFRATGRTLAFDGFYKVMGIPHSTVEQSLPDLTPGQVVGPIEIKPAQ
                     RFTSPPPRYTEASLVKRMEAEGIGRPSTYAAIIQTMLDRGYVEEVDRRLFATDKGTIV
                     TDKLVAHFPDVMDVKFTSFMEDELDKIEEAHLDWNQVLHEFYDPFHTDLVRAHEEMEA
                     AKAEPSPYKCELCGGDMVYRWGRTGRFLSCGKYPKCKGAFNIDRQGRPIRPQVVSTKC
                     EKCGKDMVLRQSRHGSFLGCSGYPECTNTIPCDQTGQPLRLVKETEIEEPCPECGVGT
                     LKVRGRGIKTFLGCDQYPRCKVTKPLPEGVRLERKAQPAVEAGVTCERCGRPMLIRKG
                     RRGEFVACSGFPRCRNTKPIEELAKLKTEAGTTTSDAHAADPASGDVSSKPARRTRRA
                     AEPGAGDSKAISEGKGSPPPGFAWTRTGKLVIETWPEVPLSCPECGRPLQLKSGRFGP
                     FFSCSNFPQCRCSVNLRGEAKKRAEIEMPPPPRPKPVPTDIVCEECGEPMMIRSGRSG
                     KFLGCSKYPKCRNTKPVPSELLLGVKSS"
     gene            6080..8098
                     /locus_tag="P5316_15185"
     CDS             6080..8098
                     /locus_tag="P5316_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86350.1"
                     /translation="MMKFFRKYTKHLLAVFMALLLVIWLAGDALQSLLLKRTTRDDFE
                     RGTLAGQKITQKDAAPVLLQLEVLDSPELRYVTEWKMVWRSVLEEMGDRDDRARYYDS
                     MSILISRGQNLIEEDEWFLLVTEARRNGVFVPNEAVEEFKVTRGLTGPSLARLRQNFP
                     LKLINEAIRSYLMVRQQAVAACTPPPISEADIRDFVRQTGEKADVVVVTLAGTEQSGF
                     YDKDYAPTDEELAELFEKHKDTASRPYAQGLDFGYQQPESVRIEYLRISADALKSQQQ
                     IDETFAYDYWNKHKNEFSREVATQSAPASGPAPAPRREQYATFTEAKAEVIGRLQKAK
                     ADEAALRIAREIIDELTESRTAKTTQPVAEQTATKAAEAEDAYAKALMKWSARYPGAV
                     SHVSLSMLEREALQKNPEIGQATARLGVQHGLSLPQAAFLVEGIADPPEQGTEQHRYF
                     RKPRETCGVPFADTKGNAFVFRTVEIRPKQAPASLDLVRERLINDARIVHAYERAGEY
                     AKALRERAATQGLEAALRNSEELKGLVDDTALSRPTPFARKTCIAIPGAAPFVRTSDI
                     PGIGSDPELIEAVFAMARDTTTQPAGHVLVWEQPGQRRWLVIELKQILPPTKADYDQY
                     RTQAVAYLRKQRQQELLVGWFDQDQIKTRMNWKSFAENRQASQGPTKG"
     gene            complement(8230..9972)
                     /locus_tag="P5316_15190"
     CDS             complement(8230..9972)
                     /locus_tag="P5316_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRR86351.1"
                     /translation="MIGVPRTFAATRTTGLAAAGPVPVRAVSSLSGCDKRSAEPDGGR
                     GVRPGVAAVIGVAFTFVLPGLLSGCASLDVRLAESVERPPRSVVVFFPDGMDVQRMDE
                     LVAEGRLPNIRERFIENGVRVRHAFSSLPTSTYPNCSSLLTGRFPGHHQIPGNFWFDR
                     RTMECRDYMTLGTCRTVNHHLAVPTLYDWLGDHFTVSIAHHTYRGVTESIDGAEGFFW
                     AWALGHYRMADSRVSIAMKEVIRRANRVGRWPSVVMTYYPGVDEIGHRFGTYSSRYAE
                     ALINVDRIVGEITALLEKSGLTESTYCILMADHGMVPARQSIDVLDWMGRVRKVRIRR
                     RSIEATAYVDRWEQMLNYDAVGGVDAGRVAMVHLRGRRGWGHRPAPQEVLDFANAQPP
                     LYELPAIQMVFLRDGPNRVRALSKNGTLAVERRLAGNEKQYRLSVYEGDPLEYLGEAS
                     LAEFVRAGWHSSGDWLAATATSRFPDFVPQVVEMFDSPRTGDMVIMSADDWLLYRRGE
                     VAGHGSCLARDMRIPLFFAGPDLPKGGRIDHARLVDIAPTILGLLGESRRLEGVSDID
                     GINLADELRRAAAP"
     gene            complement(10008..11294)
                     /locus_tag="P5316_15195"
     CDS             complement(10008..11294)
                     /locus_tag="P5316_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86352.1"
                     /translation="MQRIGKFTRRDFLAESSKTAAALSVGSLFLGQTAASAARTFGAN
                     ETIRVGVIGAGGQGTSDCAAVCGADNVVCVALCDVAEFRLAEATARITKAMESKGHKN
                     VKIDHYADYRQLLDRGDIDAVIIATPDHWHRLPFLASVEAGKHIYQEKPFSFTYEQGM
                     EMVAAAEKKPELVIQIGTQRRSDYNNAKAKQLLDDGVIGEVKFVRAKDCRNFVTGRDP
                     FAPRDVQGKIDWDKFQEPCNHKVPYDPWRYFAWRWFWDYAGGLVTDVGVHVIDIVHYY
                     MDNPVPRSAVCNGGVYGLDYWETPDVVNAVWEYDGFSLNFVSNFTNGFEGDGLTLFGT
                     KGTIDIRGSHIRVYSEGQADKPIHEFAKEGPAHQHNWVECMRTGKKPNAPVQLGFSSL
                     LPSHMANIAYRSGRKVAWDPQARKIVPWEPSSRKQS"
     gene            complement(11395..>11756)
                     /locus_tag="P5316_15200"
     CDS             complement(11395..>11756)
                     /locus_tag="P5316_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86353.1"
                     /translation="KVSFIPADFVDWAGSAYRLAPRRKAELFGCCIGHLDAGLRYTLA
                     VPTAESGIRIFLLGSLLPAIATLEVAAPGTETHPRIDRAKMGEIVALITSQTAENQAL
                     RNWYTAHRKRTLNLLFA"
BASE COUNT         2351 a         3900 c         3270 g         2235 t
ORIGIN      
        1 agtccctggg accaagaccg gcggcaacgc catctaccga atcgacactg atggttttgt
       61 caccgagatc tttcgcgagc cggtcatgat cctcggcatg gccgaggccg acggaaccct
      121 ctacgcagct accggcaacg aaggtcgaat ctacgccttg acgccgaatg ccgatcgcat
      181 cgttatgttg gccaggctcg aggccgccca ggcaacgtgc ctgctgcgcc ttccggataa
      241 tcgcctgttg atcggcacgg ccaacgcggc cacggtagtc gaactcgcag accgctatgc
      301 cgccaaggga actctgacga gcaaggtgct tgatgccgag cagatcagcg cctggggccg
      361 cgtccgctgg aacgccaccg ttccaaccgg caccaaactg agcatcgcca cgcgcagcag
      421 caacgtcgag gatgaagagt ccgaggcatg ggaggactgg tcatccgaaa tggacgccac
      481 ttatccccag tccatccttt cggccagtgc ccgcttcctg caatatcgcg tctccttcga
      541 gaccaccgtg ccggatgcaa cccccgttct gcgatccgtc gagatctttc gtatccagcc
      601 caaccgggca ccacaaataa ccagtgttga ggtgctggct gccctcgatg aggccaagaa
      661 acccaccgcc tcgccaaagg tgaaaatggc ggccggcgcc tccggcttca gcggcgggga
      721 ggaggcaagc ggtcccaaat ataactgggt cgtaaaatgg aacgccggcg atcctaacgg
      781 cgacccgctg acattcgagg tgttctatcg cgaaccgaac gcccggcgat ggattcgcat
      841 cgccaaggac ctctccgagc ccctgcacat ctgggatacc cgaactgtcc ccgacggcaa
      901 gtacgaggtt cgggttctgg ccaaggacag caaggccaac ccgcccggaa ccgacctgag
      961 cgccgcccgc atcagcgaca tgattctcgt ggacaacacg cctccggacg tgaccgtcga
     1021 ctcgatcacc ccgaaaggaa agagagcggt ccgcatcagc ttcatctgtc acgacgccct
     1081 cagccggatc gccggggcaa gctacaacgt cgatagcgac gagaagtgga ccccgttggc
     1141 gtctgatgac aacatcttcg acgagcccag tgaaaccgtc accttcacgg tcgatgatct
     1201 tgacacgggt gaacatcgga tcgctatccg ggtggaggac gaacgaggca acttccgcta
     1261 tgtgagtcgc ctcgtcaccg tcggggattg atcgcctgcg aagatgggcg acagctctcc
     1321 ggccggttaa gtggtgagcc gtaaccggag acgacaaggg tgaccaatac gacgcatgaa
     1381 agtctcgtgc ctgcgccgaa ggacgaccag cccccggcat cggcggatac gcaaagcccg
     1441 atagcagatg gccggacccg ccgtgcggcc cggtggtggt gccggcaccc ctgtccgccc
     1501 tacgcctgcc ccaccggcgc ccggcgcatc ttcatacccg tgtacggctg gctcgaagtt
     1561 gatgacacgc atcgggcacg cgtggagcac tacttccacg cgcccatgct gattctggca
     1621 ctgcttgttc tgcccatcct gctgatcgag tggtcatacg gcgatcggat caacgccgct
     1681 caccccgcag cccgcgtggc aatttgcgtc ggcggcgcaa tcatctggct cgcctttctc
     1741 gtggaattca tcataaaagt gtcgatcgcc gaatcccgct ggcggtacgt gacccggaac
     1801 tggcttgaca tcgccatcgt cgccttgccg ctcctgcgcc cgctccgtgc tctgcgcgtc
     1861 gttcgcgccg cagaggtcac gcgggtatcg cggcttttta cgatacgcgg cgttatcacg
     1921 aaactgtttc agaccgctgc ggccacggta ctcgggctcg aattcgtgcg gcggattctc
     1981 gaccgatttc gcccccctca ggcggacggc caggctcctc agtacgagcg atggtcgcaa
     2041 gcctgtctga ttgcggaaat caagcgactc aacgcgcgca tccacgaact cgaacagatg
     2101 cttcaccatt ccgctgaccg taaacgcgca gaatctgaca agacaacacc gtagtccttt
     2161 ggggtcttac ggcgaaactg ggttgtgttt tgcgcgcatg tttctacatc gcggtgcgtt
     2221 tgggtcgcgg cccgcataag ggcggtccgc cttaggagga cttgacgccg aggagcaact
     2281 ccgatggcac gggcttggta ttcctgcact tgggatactt gctgcatccc aggaacttcc
     2341 ccgatcggcc cgaacgaatc atcatcggct caccacactc ctcgcagacg atgtccgtgg
     2401 ggaccggttt aggccggggc ggaggcggca tctcgatctc cgcccgtttt ttggcctcac
     2461 cgcgcagatt gaccgagcaa cggcactgcg gaaagttgct gcaactgaaa aacgggccga
     2521 acctgccgct cttgagctgc aatggcctgc cgcactccgg gcaggacagc gggacttcgg
     2581 gccatgtctc aatcaccagc ttgcccgtgc gcgtccacgc aaaacccggc ggaggggaac
     2641 ccttgccctc actgatggcc ttcgaatccc ctgccccggg ctcagctgca cgacgagtgc
     2701 gcctcgccgg cttggatgaa acatcgcctg acgccggatc agccgcatgc gcgtcggacg
     2761 tcgtcgtgcc cgcctcggtt ttcagcttag caagctcttc gatcggcttg gtgtttcggc
     2821 accgcgggaa gccgctgcaa gcaacgaact cgccccgcct gcctttgcga atcaacatcg
     2881 gacgtccgca gcgttcgcag gttactccgg cctcgactgc aggttgggcc ttccgctcca
     2941 gacgaacacc ctccggcaac ggtttcgtca ccttgcatcg cggatactgg tcacagccca
     3001 gaaaagtctt tatgcccctt ccacgaacct tgagcgtgcc gacgccgcac tcggggcagg
     3061 gctcctcgat ctcggtttcc ttaaccagcc gcaacggttg gcccgtctgg tcgcagggaa
     3121 tggtgttcgt gcattcggga taaccgctgc atcccaggaa cgaaccatgt cgcgattgtc
     3181 gcaaaaccat gtctttgccg catttctcgc acttcgtcga caccacctga ggtctgatcg
     3241 gtctcccttg gcggtcaatg ttgaatgctc ccttgcactt gggatacttg ccgcacgaca
     3301 gaaaccgccc cgttcggccc caccggtata ccatgtcgcc cccacacaac tcgcacttgt
     3361 atgggctggg ctccgccttg gccgcctcca tctcctcgtg ggcccgaacc agatcggtgt
     3421 ggaatgggtc gtagaactca tggagaacct ggttccagtc gaggtgtgcc tcctcaatct
     3481 tgtcgagctc atcctccatg aaggacgtga acttcacgtc catcacgtcc gggaagtggg
     3541 caaccagctt gtcggttacg atcgtgcctt tgtcggtcgc gaaaaggcgt cgatcaacct
     3601 cttccacgta gccacggtca agcatcgtct ggatgattgc cgcataggtg ctgggccgac
     3661 cgatcccttc ggcctccatc cgtttgacca gcgaggcttc ggtataccgg ggaggcgggc
     3721 tggtgaaacg ctgcgcaggc ttgatctcga tcggcccaac aacctgtccc ggcgtcaggt
     3781 ccggcagact ctgttccacc gtcgaatgcg gaatccccat aaccttgtag aatccatcga
     3841 acgccagcgt gcgcccggtc gccctgaatt cggcctcccc caacacggtc ttcgcctgaa
     3901 tcagcaccgt ggtcgagtcc cattccgccg gcgtcatctg acaggccaca aagcggcgcc
     3961 agatcagctc gtacagcttt tgcaggggag cggcaagatg tcctttcaac gattccggcg
     4021 ttcgagtaac atccgtcggc cgaatcgcct cgtgcgcttc ctgcgcccgc ttgccggaag
     4081 cgtagacgtt cggttcgtcc ggcagatacc gccggccgca attctcgctg atccattccc
     4141 gcgccgcctt gaccgactcg gcgctcaggt tcgtagaatc agtgcgcatg tacgtgatca
     4201 acgccacggg accgtcctcg ccggccagct cgatcccctc atagagctcc tgagccagcc
     4261 tcatggtcct cgacgccgat aagtgcaggt gactgctcgc agcctgctgc aacgtcgccg
     4321 ttgtgaacgg cgggctcggc ttggtcagcg cgcggcgctt ctggacgtcg gtgaccgtga
     4381 aagccggcgc ttgctgccgg tcaagcctgc ccgccaatgt caccttgcgc aaaccgcgat
     4441 gctcgtactc aggccaggga acctcgttct gtcgttcgat ccgtacaccc agcgcctctg
     4501 ccgcctctct ggcctgaccg actgcgctga caaaggctct atccgcgccc gccgcagcgt
     4561 cgtatgaacc tgtgggcttg aaaggccgac catccaacgc aaccatggtg gccccaaacg
     4621 cgctatgctg agccagccat tcacccattt ccttcagcgt gcgctccttt tcgcctccgg
     4681 acaggaactc ccgccacatg ggttccagac gctcgccctc ggccgggtcc cgcgcaaagt
     4741 aagccccgat atcccagtac tcgcttggga tgaaatcctt gatctccttt tcacgctcca
     4801 cgatgagccg aagggcaact gactgaaccc gccccgccga caagcccttc gccactttct
     4861 tccacaacag cgggctcagt tcgtagccta cgatccggtc aagaatgcgc cgtgcctgct
     4921 gggcgttcac cttgtccatg tcgatgtgcc gggggtgact gaatgcttcc tggatcgccg
     4981 accgcgtgat ctcattgaat atgactcgcc tgatcttcga ctcatccagg tccagggcct
     5041 gcgccagatg ccacgcaatg gcctctccct cgcgatcaag gtccgtcgcc aggtacacgg
     5101 ccggggccct ctcggccagc ttcttcagtt cgctgaccac ctttcgccgc cctgggacca
     5161 actcgtaggt cggcgcgaag ccgcggtcga tctcgacacc aaggcctcgc tccggcagat
     5221 ctcgaacatg gcccacggaa gctttgacca cgtaccctcc acccagatac ttgttgatgg
     5281 ttttggcctt ggcaggtgat tccacgataa cgagagcttt ggaaccggcg gattctcgtc
     5341 ccttgacgcg agcctgtgcc ttgttctgct cgcccgaggt ctcagactta gtctcttccg
     5401 tcgcgactgg tgcgcctttc gcgcgcatgt ttcttgatcg cagagcgctt gcgttgcggc
     5461 ccgccttagg cttggaggtg tgtttcggac tgaccttttc cttcgccgcc atatctggtc
     5521 tcaatcatcc gagtgaacag gctgccttca agtgccgccc cccgcgaagt ctcgaaatca
     5581 taccgaatac gcggccacaa cgaccccgcc gcttaaataa cgagggcatc tcaatctcaa
     5641 tcgaccaccc gtgtgagaga ctacaacgcc tctttccggc actttggggc gtttctgatt
     5701 actgaaagtc gcgacagaaa tcaaatgata gcccgacctt acccacctta ccagttatcg
     5761 gtcttactgc gcaggccaaa gcgtccgtga cagtccagaa gtaaagatgt tatgaacact
     5821 tcaaacccca gaccataaga gcctccaccc tgcggtcttg gccgccttcc gccatggact
     5881 gccctggacc gctcatgcaa atcgggcaaa ggcccctcgc ttgctaccga gtctctcaat
     5941 gagcctcccg gacggtcaca ccttcaatgc cctgcgcccc cccgaccgtc gctccttgct
     6001 tttggcatgc acgccggtat tctgcttgat tctgcaactg gcggcgcgta tggacattgt
     6061 ttgacgggaa catactgcta tgatgaagtt tttccggaaa tacaccaagc accttctcgc
     6121 ggttttcatg gccttgctct tggttatctg gctggcaggc gatgcgctgc agagcctcct
     6181 gctgaagcgc acaacgcgcg acgacttcga gcgaggcacg ctggccggcc agaaaatcac
     6241 gcagaaggat gccgcccctg ttcttctgca gcttgaagtg ctcgactcac ctgaactgcg
     6301 gtacgtgacc gagtggaaga tggtttggag gtccgttctc gaggaaatgg gcgatcgcga
     6361 tgatcgggcc cgctactacg acagcatgag tattctgatt tcgcggggac agaacctcat
     6421 cgaggaagac gaatggttcc tccttgtaac cgaggcccgc cgaaatggag tgttcgtgcc
     6481 aaacgaagcc gtcgaagagt tcaaggtgac aagaggattg accggcccgt cgctggcccg
     6541 cttgcgacag aactttccac tcaaactcat caacgaggcc attcgctcct acctgatggt
     6601 ccggcaacag gctgtggctg cctgcacgcc gcctccgatc agcgaggccg acattcgcga
     6661 ttttgtccgc caaaccggcg agaaagccga cgtcgtcgtg gtcaccctcg caggaaccga
     6721 acagtccggg ttctacgaca aggactacgc gccgaccgat gaagaactgg ccgaattgtt
     6781 cgaaaaacac aaggacaccg ccagccggcc ttacgctcaa ggcctggatt tcggctacca
     6841 gcagccggaa agtgtacgaa tcgagtacct gcgaatcagc gctgatgccc ttaagagtca
     6901 gcagcagatc gacgagacct ttgcatacga ctattggaat aagcacaaga acgagttttc
     6961 ccgggaagtc gccactcaat ccgcccccgc ttcaggcccg gcgcccgcgc cgcggcgaga
     7021 acaatatgcc acctttactg aggccaaggc tgaggtcatc ggccgtctcc agaaagccaa
     7081 ggcggacgaa gccgctctgc ggatcgctcg cgagatcatc gacgaactca ccgaatcgcg
     7141 gaccgccaag acgacgcagc ctgtcgctga acaaaccgca acaaaagcgg cggaagccga
     7201 ggatgcctat gctaaggccc tcatgaaatg gtcggccagg taccctggcg ccgtcagcca
     7261 tgtctcactg tccatgctcg aacgggaggc cctccagaag aaccccgaaa tcggccaggc
     7321 aaccgcgagg ctcggcgtgc aacatgggct ttcgctcccg caggcagcct tcctggtcga
     7381 gggaattgcc gatccgcccg agcagggaac ggaacagcat cgttacttcc gcaagccccg
     7441 cgaaacctgc ggcgtcccat ttgctgacac aaagggtaac gccttcgtct ttcgcacggt
     7501 ggaaatcaga cccaaacagg ccccggcaag cctcgacctt gtccgcgagc gcctgatcaa
     7561 cgatgcacgc atcgtgcacg cctatgaacg cgcgggcgaa tacgccaagg cccttcgcga
     7621 acgtgccgca acacaaggac tcgaagctgc cttacgcaac agcgaagaac tcaaggggct
     7681 ggtggacgat acggctctca gccgtccgac acctttcgcc cgcaagacct gtatcgccat
     7741 ccccggtgct gcgccctttg tgagaacaag cgatataccg gggattggct cggatcccga
     7801 gttgatcgag gcggtcttcg ccatggcccg tgataccacc acgcaacccg ccggccatgt
     7861 cctcgtctgg gagcaacccg gacaacggcg ctggcttgtt attgagttga agcagatcct
     7921 cccccccacc aaagccgact acgaccagta ccgcacccag gccgtcgcat atctgcggaa
     7981 gcagcgacag caggagctct tggtcgggtg gtttgaccag gaccagatca agacccgtat
     8041 gaactggaag tccttcgcgg agaaccggca ggcctcgcag ggccccacga aaggctagcg
     8101 gccgtccgcg aacgacaaaa cgacaagccc accaagtact gcttgcgaag aacgaaggtc
     8161 tatccacagg ccccggatct gcaatctggt tggatgccgg ccgttttcgt cctgagccga
     8221 agtccctggc tacggcgcgg cggctcggcg caattcatcg gccagattga ttccgtcgat
     8281 gtccgaaacg ccttccagac gccgggattc acccagcaat ccaagaatgg tcggcgctat
     8341 atcaaccaga cgcgcatggt cgatccggcc gcctttgggc agatccgggc cggcaaagaa
     8401 gagcggaatt cgcatgtccc gggccaggca cgaaccgtga cccgccacct ccccgcgccg
     8461 gtacaacaac cagtcgtcag ccgacatgat caccatgtcg ccggttcgcg gcgagtcgaa
     8521 catctccacc acttgcggaa cgaagtccgg gaatcgcgac gtggccgtgg ccgccagcca
     8581 atcgccagaa gaatgccatc ccgcccggac aaactcggca agagatgcct cgcccagata
     8641 ctcgagcgga tccccctcat acacgctcaa ccggtactgc ttttcattcc ccgctaatcg
     8701 tcgctccact gccaacgtgc cgtttttcga caaggctcgc acccggttcg ggccgtcgcg
     8761 caaaaacacc atctggatcg ccggcagttc atacaaaggc ggctgcgcgt tggcgaaatc
     8821 cagcacctcc tgcggcgcgg gtcgatgacc ccacccgcgc cgaccgcgca ggtggaccat
     8881 tgcgacacga cccgcatcga cccctccgac ggcgtcgtag ttcaacatct gctcccatcg
     8941 atcgacgtac gccgtcgcct caatcgaacg ccgccgaatg cgtacctttc gtaccctgcc
     9001 catccagtcg agaacatcga tcgattgcct ggccggcacc attccatggt cggccatcag
     9061 aatgcagtag gttgactccg tcagtcccga tttctccagc agagcggtga tctcacccac
     9121 gatgcgatcg acattgatga gggcctccgc gtagcgggac gagtacgtac cgaaccgatg
     9181 gccaatctcg tcaacacccg ggtaataagt catgaccacc gacggccatc gcccgacgcg
     9241 attggccctg cgaatcactt ccttcatcgc gatgctcacc cgggaatccg ccatcctgta
     9301 gtggcccagg gcccatgccc agaagaagcc ctccgctccg tcgatcgact ccgtcacgcc
     9361 ccggtaggtg tggtgggcga tactgacggt aaaatgatcg cctagccagt catacaacgt
     9421 cggcaccgcc agatgatggt tgaccgtgcg gcaggtcccg agcgtcatgt agtcccggca
     9481 ctccatcgtt cgccggtcaa accagaaatt acccggaatc tggtgatggc ctgggaatcg
     9541 accagtcaac agtgatgagc agttgggata ggtgcttgtg ggcaggctgc tgaatgcatg
     9601 ccgcacccgg acgccgttct cgatgaaccg ctcacgaatg ttgggtagtc gcccctcggc
     9661 aacaagttcg tccatacgct gcacgtccat gccgtcaggg aaaaaaacga cgacggaccg
     9721 cggcggacgt tcgaccgact ctgcaagccg tacatccagg gaagcgcagc ctgacagcaa
     9781 tcccggcagc acaaacgtga aggcaacccc gataaccgcc gcaaccccag ggcgaacgcc
     9841 acgaccaccg tccggctcag cggaacgctt gtcgcaaccg gagaggcttg agacggctcg
     9901 aactggaacc ggccctgcag ccgcaagccc ggttgtccga gtcgccgcga atgttcgagg
     9961 tacaccgatc atcaacgggt gttccgccga ccgcctggga agacctgtca cgattgtttc
    10021 ctggaggacg gctcccaggg aacgatcttc ctggcctgcg ggtcccaggc aaccttccgg
    10081 ccgctccggt aagcgatgtt cgccatgtgt gacggcagca gcgagctgaa gccgagttgg
    10141 acgggggcgt tgggtttctt accggtccgc atgcactcca cccagttgtg ctggtgagcc
    10201 ggcccttcct tggcgaattc atgaatgggc ttgtcggctt ggccttcgga atacacgcgg
    10261 atatgtgaac cgcgaatgtc gatggttccc ttcgtgccga acagcgtcag gccgtcgccc
    10321 tcaaaaccgt tggtgaaatt gctcacgaaa ttcagcgaga agccgtcata ttcccagacg
    10381 gcgttaacca cgtcgggcgt ctcccagtaa tcgagcccgt acaccccgcc gttgcatacc
    10441 gcgctcctgg gaacggggtt gtccatatag tagtgaacaa tatcaatgac atggacgccg
    10501 acgtccgtga ccaggccgcc ggcgtaatcc cagaaccatc gccacgcgaa gtaccgccac
    10561 gggtcgtaag gcaccttatg gttgcatggc tcctggaact tgtcccagtc aatcttgccc
    10621 tggacgtcgc gaggcgcaaa cggatcgcgg ccggtcacga agttccggca gtccttggcc
    10681 cgcacgaact tgacctcacc gatcacaccg tcatcgagga gttgcttggc cttggcgttg
    10741 ttgtagtcgc ttcgccgctg cgtgccgatc tgaatgacca attcgggctt cttctccgcg
    10801 gcggccacca tctccatccc ctgctcatag gtgaacgaga agggcttctc ctggtagatg
    10861 tgcttgcccg cctccaccga agccagaaac ggcaggcggt gccaatgatc cggcgtcgca
    10921 atgatgaccg catcgatgtc cccgcgatca agcaactgcc ggtagtccgc gtagtggtct
    10981 atcttgacgt tcttgtgccc cttgctttcc atcgccttgg tgatccgggc ggttgcctcg
    11041 gccagacgga attcggctac gtcgcaaagc gctacgcata ccacgttatc cgccccgcaa
    11101 accgccgcgc agtcgctcgt gccctgtccg ccggccccga tgacgccgac ccggatcgtt
    11161 tcattggccc cgaaggttcg ggccgccgag gcagccgtct gccccaggaa cagcgaaccc
    11221 acgctcaagg ctgcggccgt cttcgatgac tcggccagaa aatcacgtcg cgtgaattta
    11281 ccgatgcgtt gcatgtctgc ctccattcgt cacgacacaa tgcgacatcc tccaccccgc
    11341 cgtccgatac cacgcccact gacggaagac attctaccca ttgccgggcc gatgtcacgc
    11401 aaacaacaga ttcagcgttc gcttgcggtg ggccgtgtac cagttacgca acgcctggtt
    11461 ctcggcggtc tgactggtaa tgagagcgac aatctccccc atctttgccc ggtcgatcct
    11521 gggatgggtt tcggttcccg gtgctgcgac ctccagcgtc gcaatggccg gcagcaacga
    11581 acccaacaga aaaatgcgaa taccgctctc ggccgtcgga accgccagcg tgtaccgaag
    11641 gcccgcgtcg aggtgcccga tacagcaacc gaacaactcc gccttccgcc gcggggccaa
    11701 acgataggcc gatcccgccc aatccacgaa gtccgcagga atgaagctga cctttc
//
LOCUS       DAOTPW010000177        11740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5208_length_11740_cov_5.535387, whole genome shotgun sequence.
ACCESSION   DAOTPW010000177 DAOTPW010000000
VERSION     DAOTPW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_11740_cov_5.535387"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..1783
                     /locus_tag="P5316_15205"
     CDS             476..1783
                     /locus_tag="P5316_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86354.1"
                     /translation="MNRREFLTRSAGIVTAAAIGEPALASLDEAKHSKSVRLGVVGLG
                     NRGTHLLRLALAVPGVQVRAVCDLNEDNLGRAAEIVKKAQGSEPFRYSKAPDDYGAMV
                     RRDDLDAVLIATPTKWHCPMAIEAMKAGKHIGSEVPAGFQLRELWDLVKTKEATGRRY
                     MLLENYLYMRHIMTVWNMVRAGIFGEPYYAECGYLHDCRFMLFKQDGSLDWWGDWAAN
                     NFGSDYPTHAMGPVSKWMGLNDGDRMEYCTSMMTAPRVLKEYAVKKYGADSPQGKIDW
                     ALGEFVSTQIQTVKGRVIRLDYDVNSPRPAVLPYMIQGTKGLYDSRHGVYIEEEGKGE
                     KWDNLQKYQEQYDHAYWKRDARQAENAGHGGGDFFAVRDFIEMVRQDREPWIDVYDAA
                     SWSVLYWCSHESIANRSKAVDIPDFTNGRWQDPDWRKDNHRPA"
     gene            1902..2582
                     /locus_tag="P5316_15210"
     CDS             1902..2582
                     /locus_tag="P5316_15210"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRR86355.1"
                     /translation="MTTRLTTTVIIVATSLAGGCTEPLFVTHRPAATPTDTVLPPPAT
                     SGGMSLAKAMADRRSVRSFADKPVTLEQVSQLCWAAQGITDQSSGKRTAPSAMALYAV
                     NIYLVNKDGCFEYLPGPHALRPMGGPEAMGRLRGACWQPSVGSAPLCMIITIDVERLK
                     PRCGKMAEQYSLLEAGHVAQNVLLQATALELASVPVGGFDANAVGKAMNIPAPQRAVY
                     LLPIGCKK"
     gene            complement(2735..3211)
                     /locus_tag="P5316_15215"
     CDS             complement(2735..3211)
                     /locus_tag="P5316_15215"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRR86356.1"
                     /translation="MAYYGAVGKAGRTVVARLRPGTDLLGGLKELAEANKIKAAYIPV
                     IIGGVDRAIIEVAKPSKESPVGVVAKKIELTGPIAICGAQGMICELEDGGIEPHLHIT
                     FVDSNGHVHGGHLEAGTAPVTTTADVIMQEVLEVKFGRGFDPDIKTTLFFPSPAGQ"
     gene            3414..4535
                     /locus_tag="P5316_15220"
     CDS             3414..4535
                     /locus_tag="P5316_15220"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRR86357.1"
                     /translation="MRIPTTSRAFVVDGTGKGSIQEIPIPKLGPNDALIRMEGVYGCA
                     GGDTIVYSGKHPHSIGKYPLILGHEGVGVVAAIGPEAAHRRGLKVGDRVAVEVMIPCD
                     TNSPDACEQCRAGEYQLCEKNRQHGVSMPLSLEHGLWGMYSDFLMVHPRAITHRFPDN
                     VNMANAVATAFLANAVRWTEVKGKVQAGEVVAIFGPGPQGVGSAAVATYRGALPILIG
                     LSTDQARFDIAVGIGVVKAEHIIRADKEDVVEKVKSLTDGKGCHLSIECSGADPAVGP
                     WLKVLRKRGRGVICGYHGGREITVPIDQIATKELNIQGAWGQAGGWQLAIEMLGKRVF
                     DFAPMITNRYKIDQLNEVVPMLKDPSKITMKALFEPLPL"
     gene            4724..5551
                     /locus_tag="P5316_15225"
     CDS             4724..5551
                     /locus_tag="P5316_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR86358.1"
                     /translation="MFVKTDDPEELALAHRKLGYRAAYCPGVNLDDRERIKAVREAYA
                     KHDVVIAEVGRWCNLLDADPAKRKANLEAVTEGLALAEAIGALCCVDIAGSFSAESWF
                     GPHPDNLSQRFFDAAVENARKIIDAVKPKKARFCYEMMGWALPDSPDSYVRLIKAIDR
                     PAFGVHLDPCNLINCPERFYHNADLLDECFDKLGRWIVSCHAKDLVWKLEMNIHFQEV
                     PPGSGRLDYRTFLRRLAALDHDTPLMIEHCANEAEYTRGREYILGLGPKTGVDFGSA"
     gene            5815..6924
                     /locus_tag="P5316_15230"
     CDS             5815..6924
                     /locus_tag="P5316_15230"
                     /inference="COORDINATES: protein motif:HMM:NF023462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HRR86359.1"
                     /translation="MKILALEPYHGGSHKAFLSGWSSNSRHEWTVLGLPAYKWKWRMR
                     HAAITFADQANQLLKSGSTWDLIYCSDMLNLAEFLGLLPVEMRHLPTLVYFHENQLTY
                     PVRFETDRDFHYVLSNIITALAAKRVWFNSAYHRDSFLEGLPAFLKRMPDYQPFDAPD
                     RIRAKSDVRPQGVQEFPKRGERMPGPIRILWAARWEHDKNPDAFFLALKSLKLGGVDF
                     RISVIGEQFRERPPIFDWARQYFFHHIDRWGFQESRQDYEEALMEADVFVSTANHEFF
                     GITAVEAIAAGAYPLLPKRLAYPEILDPKVAGDVSCFFYEREAEGLAERLRELAERLT
                     KGDLWQGDPDRAKRATARFSWSKLAPILDEAIESV"
     gene            complement(6997..7602)
                     /locus_tag="P5316_15235"
     CDS             complement(6997..7602)
                     /locus_tag="P5316_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRR86360.1"
                     /translation="MRREWQYSVSALAALVFAALVGCSGGVLPSGPAGTGAQEVFNLL
                     RLDGDVADTSGSLAAEEPCLELGAPDTAVQKRLFAELNNYRVNHGLSPLVYSKRLEAA
                     AQAHLSDMYERGYFAHITPEGLEPGDRAMELGFCHRYVGENLAAGQRTVQAVMRAWDN
                     SPSHQLNMLEPDYVYVGLGHFVDPITGRQYWAQEFAYHVVE"
     gene            complement(7773..8444)
                     /locus_tag="P5316_15240"
     CDS             complement(7773..8444)
                     /locus_tag="P5316_15240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR86361.1"
                     /translation="MRTRILGLFVLALVMTALPSCNSSADETDSSATAPPDYKPPAKA
                     AEPKPAPKPAPAAATPASRPTNTQVVIETNMGTMVVRLDDQRTPITVANFLQYVDDKF
                     YDNTIIHRVERNFVIQGGGFDTTLREKETRPPIINEGKKGRQNTRGTIAMARTSEPNS
                     ATCQFYINLKHNATLDGGYCAFGEVIEGMDVVDKIGAVPTENRGGAFTAIPIEQVVIR
                     SIRRK"
     gene            complement(8512..10539)
                     /locus_tag="P5316_15245"
     CDS             complement(8512..10539)
                     /locus_tag="P5316_15245"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HRR86362.1"
                     /translation="MPGVPLRLGDRDILYLTGVTAHLSRRSVEHMIVPRPLAQSPGVG
                     ELVDDVVVSHALAAMAGGAPEMTCQIVSRDNGRQVTVAVESRSRLPQLSLTAVLPADV
                     HPARYRPPEGFRATQTAIGPATRLLFDSLYDGSGGTYATYSGDVSIESSYGGYRLKAR
                     TDATMNKKVVLLRLHLQVSDHGEGTSLRPPLPVERLAPPVPLKEARRLASEWAVAPSA
                     EVTAMTWLTEALNRLPVDRFELLRRTCPLQPIRRVDRFGDTTAWNLAIGRPPEQWNVL
                     LLSNPSAVAKTETIGLKRLGISPPARSRLAVYDFWQQQLLAVTDEVFETDIPPGDCRV
                     LCVRDVRPNEPRVLSTSRHITQGLTDLTDVSYDPQSMTLSGRSNLFPDEAYELRILLP
                     IDRNSLEIATVDANMVSSLIRSHGPLRMVTLQSNTEQTVSWSIAFRRASLPLSPPPSP
                     ARMSTQQNTRGVLLSWDAAESRPARYRIYRDGKPLATVAGFENQYQDSDIVYDTSYAY
                     TMRAVDWAGQESPPLPEGVHRTPVPANAYLTQLVPLFIGGPAPSADRNVGGNPLRMAG
                     RRYHRGLGTVPGNRIDYLLGKGYDRFSGEVGIDDATTAKGRARFEILADGHLLFSSES
                     LAVGDKPQRFNVPVNGCRRLTLAVVRVDDGDGQVHANWGDTYLRASATPGR"
     gene            complement(10749..>11740)
                     /locus_tag="P5316_15250"
     CDS             complement(10749..>11740)
                     /locus_tag="P5316_15250"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRR86363.1"
                     /translation="ERLFALIDKLKARGVTLIYVSHRLEEVFRLCDRVTVLRDGQHIE
                     TADVSDMTTDKIVQRMIGRPLEQYFPAHLDSRPGSERLRIEDLSSPGKFRNISLTVRA
                     GEVVGLAGLVGAGRSEVAQAVFGLDPKATGRVLIDGRPVHIRSPREAMALGVGFLPED
                     RKTQGLVLSMGGRANLSLPILNRLCRAGFVRTGYEKALTRRYFDRLRVRTPHIDAPVW
                     SLSGGNQQKIAMAKWLASRCAILLIDEPTRGVDVGAKAEIHALIDELAREGAAILLIS
                     SELPEVLNLSTRIIVLREGRQVGELSRAEASQESLMRLMAGVKTSLTNWPLPL"
BASE COUNT         2377 a         3568 c         3560 g         2235 t
ORIGIN      
        1 acggaatcgc gacggccgtg ttctccggat cattcatcct gacccaagcc gcgaggaccc
       61 gtttcgactg ggcccaccgc aatgccggtt acctgcgtcc gggtttcgaa tgaggttgct
      121 catgcaagac ccacgggcgg tcggcgcgat caccggccgt cggcggacac gttccgtgat
      181 tcggtctgaa ccagcctcag agaaaatgat gtccgccaga acaacgacgc caagaacgcg
      241 ctgtaataag aaatatatag gaggttttat gtgacattcg cgagacggac cctggcgtat
      301 cggacggccg atcatctcag acacaactcc gaacatgaat gcatttgatg ccgacttccc
      361 tgttccccgt gccgcaacag ggccgtgtcc tgacgtggac caaggcgatc cggttgcggt
      421 tatgatcgta tgagcgataa ttgaacgcgc tgtactgttc tgcgggagaa actggatgaa
      481 tcgacgagag ttcctgacca ggagtgcggg aatagtgacc gccgcggcga tcggtgaacc
      541 ggcgttggcg tctcttgatg aggcaaagca ctccaaatcg gtacgcctgg gtgtcgtggg
      601 cctgggcaat cgcggcacgc accttcttcg gttggcactt gccgtgcccg gcgtgcaggt
      661 cagggcggtg tgtgacttga atgaggacaa tctcgggcgt gccgctgaga tcgtcaagaa
      721 ggcacagggt tccgagccgt tccgctattc gaaggccccc gatgactacg gcgccatggt
      781 cagacgcgac gacctcgacg ccgtgctcat cgccacgccg accaagtggc attgtccgat
      841 ggccatcgag gcgatgaagg cgggtaagca catcggcagc gaggtgcccg cggggttcca
      901 gctccgggag ctgtgggatc tcgtcaagac caaggaagcc accggccggc gttacatgct
      961 gctcgagaac tatctctaca tgcggcacat catgaccgtc tggaacatgg tcagagccgg
     1021 catctttggc gagccgtact acgccgaatg cggttacctc cacgactgcc gcttcatgct
     1081 tttcaagcag gacggctcgc tcgactggtg gggcgactgg gcggccaaca acttcggcag
     1141 cgactatccg acgcacgcca tggggccggt cagcaagtgg atggggctca acgacggcga
     1201 ccgcatggag tattgcacga gcatgatgac cgccccgcgg gtcctgaagg aatacgcggt
     1261 caagaaatac ggcgccgaca gcccgcaggg caaaatcgac tgggcgttgg gcgagtttgt
     1321 ctcaactcaa atccagacgg tcaaggggcg ggtgatccgg ctcgactacg acgtcaattc
     1381 gccccggccc gccgtcctgc cttacatgat ccaaggcacc aagggactct acgactcgcg
     1441 gcacggcgtg tacatcgaag aggaaggcaa aggcgagaag tgggacaacc tgcagaaata
     1501 ccaggagcag tacgaccacg catattggaa gcgcgacgcg cgacaagctg agaacgccgg
     1561 ccacggcggc ggcgacttct ttgccgtcag agatttcatc gaaatggttc gtcaggatcg
     1621 cgagccgtgg atcgacgtgt atgacgcggc ctcatggagc gttctctatt ggtgttccca
     1681 cgagtcgatc gccaaccgga gcaaagcggt tgacatacct gattttacga acggacgctg
     1741 gcaggacccg gattggcgaa aggataacca tcgaccggcg tgaacaagag aggccaaggg
     1801 tcgtggggcc aggccgagaa gacagaagca tacatgcaac aggtgggatt gcaggttcca
     1861 ccttggcgga tcgtttcctc tcatgaaagg accggtgtga catgacaacg cgactgacaa
     1921 caacagtaat catcgtggcg acgagtcttg ccggtggttg cacggagcct cttttcgtaa
     1981 cgcatcgacc ggcggcgacc ccgaccgaca ccgtcttgcc gccccctgca accagtggcg
     2041 gcatgtctct ggccaaggcc atggccgacc ggcgatcagt ccgctcgttt gctgacaagc
     2101 cggttaccct ggagcaggtc tcgcagttgt gctgggcagc ccagggcatc accgaccagt
     2161 caagcggcaa gcgcaccgct ccctcggcca tggccctgta cgcggtgaac atctacctgg
     2221 tcaacaagga cggctgcttc gaatacctgc ccggcccgca cgccctgcgg ccaatgggtg
     2281 ggcctgaagc gatgggccgg ctccgcggcg catgctggca gccgtcggtt ggctcggcgc
     2341 ccctgtgcat gatcatcacc atcgacgtcg agcggctcaa accccgttgc ggcaagatgg
     2401 ccgaacagta cagcctcctg gaggcaggac acgtggccca gaacgtgttg ctccaggcaa
     2461 ccgctcttga actcgcaagc gtgccggtgg gcggattcga cgcgaatgcc gtcggaaagg
     2521 caatgaacat cccggctccg cagcgggcgg tgtatcttct gccgatcggc tgcaagaaat
     2581 gagttcccgc tcgagtgctc gcagcaagaa ccagacttgc tggttgaagg gcggcctcaa
     2641 atcagcatga cgacctgggt ttccggcact gccggcctgt cgttttagag gttcgcgcga
     2701 aacggttgac tgcatgtgcc ggtgctcgag tcgactactg cccggccggg ctgggaaaaa
     2761 acagcgtcgt cttgatgtcc gggtcaaagc cccgcccgaa tttgacctcc aggacctctt
     2821 gcatgatcac gtcggcggtc gtggttacgg gggcagtgcc ggcctccagg tgcccgccgt
     2881 gcacgtgccc gttgctgtcc acaaacgtga tgtgcaggtg cggttcgatt ccgccgtcct
     2941 ccagttcgca gatcattccc tgggctccac aaatggcgat cgggccggtt agttcaatct
     3001 tcttggcgac cacgccgacc ggactctcct tcgagggctt ggccacctcg ataatcgccc
     3061 ggtccacccc gccgatgatg accggaatgt aggccgcctt gatcttattg gcctcggcaa
     3121 gttctttcag cccccccagc agatccgttc caggccgaag tcgcgccacg actgttcgtc
     3181 ccgccttgcc cactgcaccg tagtatgcca tgatgacctc actgcttaat ctgagattcc
     3241 aaatccacgc aaactcagca actccggctt gtcgctcgcg tacgccggcc ttgagtcctc
     3301 aagtccgcaa gtctcgatcg ccgcgcatcc ggtcgccggt cgcgactgta cacgaccggc
     3361 cttcggggcg atattatgac cctttggtga ccgattcgcg agaggcaaga cagatgagaa
     3421 taccaacgac aagccgggcg tttgtggttg acggcacggg caaaggcagc atccaggaaa
     3481 tcccgattcc caagctcggc cccaacgacg cgctgatccg catggaagga gtctacggct
     3541 gcgccggcgg cgataccatc gtctacagcg gaaaacatcc tcattcgatt ggcaagtacc
     3601 cgctgattct gggacacgag ggcgttggcg tggtcgcggc catcggtccc gaggccgcac
     3661 acaggcgagg cctcaaggtg ggcgaccgag tggccgtcga ggtcatgatt ccttgcgaca
     3721 ccaatagccc cgacgcctgt gaacagtgcc gggcaggcga gtaccaactc tgcgagaaga
     3781 accggcaaca cggcgtgtcg atgccgctat cactggaaca cggcctgtgg ggcatgtact
     3841 ccgacttcct gatggtccat ccgcgggcca tcacccaccg ctttcctgac aacgtgaaca
     3901 tggctaacgc ggtcgctacc gccttcctgg ccaacgcggt ccgctggacg gaggtcaaag
     3961 gcaaggtcca agcgggcgag gtggtagcga tcttcggccc cggcccacag ggcgtgggca
     4021 gcgcggccgt tgccacgtac cgaggtgcat tgcccatcct catcggactg agcaccgacc
     4081 aggctcgctt cgacattgcc gtgggaatcg gagtggtcaa ggccgaacac atcatccgag
     4141 cggacaagga agacgtggtc gaaaaagtca aatcgctgac cgacggtaaa ggatgtcatc
     4201 tgtccattga gtgctcgggt gcggacccgg cggtaggccc gtggctgaag gtgctacgca
     4261 agcgaggacg aggcgtcatc tgcggctatc acggcggccg cgagatcacc gttcccatcg
     4321 atcagatcgc caccaaggaa ctcaacatcc agggcgcgtg gggccaggcc ggcggctggc
     4381 aactggccat cgagatgctg ggcaaacggg tcttcgactt cgcccccatg atcacgaacc
     4441 gttacaagat cgaccagttg aacgaggtgg tgcctatgct caaggacccc tccaagatta
     4501 ccatgaaggc actattcgag ccgttacccc tgtagaatcc cggcagaccg aagcagtgag
     4561 atgggagccg acgatgcaga gaaaacgaat gaatcgacgc aacatgctga aaggagctgt
     4621 gggggttggc gtaactacgg ctcttggaac caggcgcctc gcagcggcaa gtgcgcccga
     4681 gtccgatgcg aagaagcgcc gggtaatccg cttggggggc cctgtgtttg tcaaaacgga
     4741 cgaccccgag gaactggccc tggctcaccg caagctgggc taccgcgcag cctattgccc
     4801 gggcgtcaac ctcgacgaca gagagcggat caaggccgtc cgtgaagcct acgccaaaca
     4861 cgatgtagtc atcgccgagg tcgggcgttg gtgcaatctt ctggacgccg atccggccaa
     4921 gcggaaggcc aatctcgaag cggtgaccga agggctggcc ctggccgaag cgatcggggc
     4981 attgtgctgc gttgacatcg ccggatcttt cagcgccgag tcatggttcg ggcctcaccc
     5041 cgataacctt tcgcagagat tcttcgacgc cgcggtcgaa aacgcccgca agatcatcga
     5101 tgcggtgaag cccaagaaag cccggttctg ctacgagatg atgggctggg ccctgcccga
     5161 cagtccggac tcctatgttc ggttgatcaa ggccatcgac cgcccggcct ttggggtaca
     5221 tctcgacccc tgcaacctga ttaactgccc ggagcgcttc taccacaacg ccgatttgct
     5281 cgacgagtgc ttcgacaagt tggggcggtg gatcgtaagc tgccatgcca aagatctggt
     5341 ttggaagttg gagatgaata tccacttcca ggaggtcccg cccggatcgg gcagactcga
     5401 ttacaggacg ttcctccgac gcttggccgc cttggatcat gacaccccac tcatgatcga
     5461 gcattgcgcc aacgaggctg agtacactcg gggccgtgaa tacatactgg gtctgggtcc
     5521 caaaaccggc gttgacttcg ggtcggcgtg aatagctctc cggactgcgg ccaagccccg
     5581 gaaaccatac gagaaaccgt cttggcacct gcaaaagcga cgccggcggt tgactgatga
     5641 aatctcaggc tggtcggcat tcgtttctgg tgcaaacccg gacaaaagac agtatactcc
     5701 ttggttctga gggatgggcg tttgccccga cgggccgggc cgggtggctt ggatgctggc
     5761 caagcggtaa catgtgccca cacgaagcgg tgatgacgac gcggtgagaa catcatgaaa
     5821 atcctggctc ttgaacctta ccacggcggg agtcacaagg ctttcctcag tggctggtct
     5881 tccaacagtc ggcacgaatg gaccgtattg ggattgccgg cctacaagtg gaaatggcgc
     5941 atgcgccacg ccgccatcac ctttgcggac caggccaatc agcttctcaa atccggctca
     6001 acttgggatc tcatttattg ttccgacatg ctcaatctcg ccgagttcct gggcctgctc
     6061 ccggtggaga tgcgacatct gccgaccctc gtatacttcc atgaaaacca gctcacgtat
     6121 cccgtccggt tcgaaaccga ccgggatttc cattacgtcc tgagcaatat catcactgct
     6181 ctggcggcca agcgcgtatg gttcaactcg gcgtaccacc gcgattcttt tcttgaaggt
     6241 ctgccggctt tccttaaacg catgccggac tatcagccgt tcgacgcccc ggaccggatt
     6301 cgagccaaga gcgacgtccg cccgcagggt gttcaggagt ttcccaagcg gggtgaacga
     6361 atgcccgggc ccatccgcat tctctgggcc gcgcgttggg aacacgacaa gaatcctgac
     6421 gccttctttc tggctctcaa gtctctcaag ctcggcggcg ttgatttccg catcagcgtc
     6481 atcggcgagc agttccgcga gcgtccgccg atctttgact gggcccggca gtatttcttc
     6541 caccacatcg atcgctgggg ttttcaggag tcgcggcagg attacgaaga agctctgatg
     6601 gaggccgacg ttttcgtctc gacggccaac cacgagttct tcggcatcac cgcggtcgaa
     6661 gcgatcgccg ctggggccta tcccctcctg cccaagcgac tggcttatcc ggagatcctc
     6721 gatcccaagg ttgccgggga cgtctcgtgt ttcttctacg aaagggaagc cgaaggtctg
     6781 gccgagcggc tgagagaatt ggctgaacgc ctcaccaagg gcgacctctg gcagggcgat
     6841 cccgatcggg ccaagcgagc caccgctcga ttctcctgga gcaagttggc tcccatcctg
     6901 gatgaagcga tcgagtcggt gtgaacggtg accgggcgca tgagaacggc cgggaataac
     6961 ccggccgtct cctcctcgtc tcgatctcgc gattcgttac tcgacgacgt ggtatgcgaa
     7021 ttcctgggcc cagtattggc gccccgtgat cggatccacg aagtgcccca gccccacata
     7081 cacgtagtcg ggttccagca tgttcagttg gtgcgacggg ctgttgtccc aagctcgcat
     7141 gactgcctgc acggtccgtt gcccggccgc cagattctca cccacatacc tgtggcagaa
     7201 gccaagttcc atcgcccggt cacccggttc aagtccctcg ggcgtgatat gggcgaagta
     7261 accgcgttcg tacatatccg acagatgagc ctgagccgca gcctccagac gcttgctgta
     7321 aaccagcggc gacaacccat ggttgacgcg atagttgttc agttcagcaa acagacgttt
     7381 ctgaacggcg gtgtctggag caccgagctc aagacagggt tcctcagcgg ccagactgcc
     7441 gcttgtatcc gccacgtccc cgtcaaggcg caacagattg aacacttcct gcgcgccggt
     7501 gccggccggt ccactgggca gaacgccgcc gctgcagccg accaacgctg cgaagaccag
     7561 ggcagcaagt gctgaaaccg aatattgcca ctcgcgacgc atcgtgctgt tgtccacaaa
     7621 gtgccggcga atcgcacttt gccgatcata tcacgacggt caccccgaag tcatcaacca
     7681 gggcgcagga aatgttgaaa aacgcttcgg gcgctttctt tccggcttct ttatgggccg
     7741 gaaaacaata tcggaccatg cggccatttt gcctattttc tccggattga ccggatcacc
     7801 acctgctcga tagggatggc cgtgaacgcg ccgccgcggt tctctgtcgg aacggcacca
     7861 atcttgtcca cgacgtccat gccttcgatg acctcgccga aagcgcagta cccgccatcc
     7921 aaggtcgcgt tgtgcttgag gttgatatag aactggcagg tggctgagtt gggctcggaa
     7981 gtacgggcca tggcgatggt gccgcgagta ttctggcgtc ctttcttgcc ttcgttgatt
     8041 atcggcggac gcgtctcctt ttcgcgcagg gtggtgtcaa aaccgcctcc ctggatcacg
     8101 aaattcctct ccacccgatg gataatggtg ttgtcataga acttgtcatc aacatactgc
     8161 aggaaattcg ccaccgtgat tggcgtgcgc tggtcatcga ggcgcacaac catcgttccc
     8221 atgttggtct cgatgaccac ctgtgtgttg gtcggtcgag aggcaggagt ggcagcggcg
     8281 ggagccggtt tgggcgcagg cttgggttcc gcggctttcg ccggcggctt atagtccggc
     8341 ggcgctgttg cggaagaatc ggtttcatcc gccgatgaat tgcatgacgg cagagccgtc
     8401 atgaccaagg ccagaacgaa taatcccaga atgcgtgttc tcatctgaag acctccatcc
     8461 ggacacaggc gcgtttgtcg gttaccatga taacgttaat acgggatcat gtcatcgacc
     8521 gggtgtggcc gacgctcgca gatacgtgtc gccccagttg gcatgcactt ggccgtcgcc
     8581 gtcgtcaacc ctgacaacgg ccagcgtcag tctccggcat ccattgacgg gcacattgaa
     8641 tcgctgcggc ttgtctccga ccgccagcga ctcgctgctg aagagaagat gaccgtcggc
     8701 aaggatctcg aaccgcgcgc gccctttggc cgtcgtggca tcatcgatgc ccacttcccc
     8761 gctgaagcgg tcgtatccct tgccgagcaa gtagtcaatg cgattgcccg gcacggtgcc
     8821 caggcctcga tgatagcggc gtccggccat tcgcagcggg ttacctccga cgttgcgatc
     8881 ggccgaggga gcagggcccc cgatgaagag cggcacaagc tgcgtcaggt acgcgtttgc
     8941 ggggaccggt gttcgatgca caccctcggg cagaggcgga gactcctgcc ctgcccagtc
     9001 taccgctcgc atcgtgtaag catatgaagt gtcatagacg atatcgctgt cctggtattg
     9061 gttctcaaag ccagccacgg tggccagcgg tttgccgtcg cggtagatgc ggtatcgcgc
     9121 cggccgcgac tctgctgcgt cccatgacag caagacgccg cgggtgtttt gttgcgtcga
     9181 cattcgagcc ggggaaggtg gaggcgacag cggcagcgac gcccttcgga atgcgatgct
     9241 ccatgaaacg gtctgctcag tattgctctg cagcgtgacc atgcgcaacg gtccgtgaga
     9301 tcgaatcaac gacgagacca tgttcgcgtc cactgttgcg atctcgagcg agttccggtc
     9361 gatcggcaac agtatgcgca attcatacgc ctcatccgga aacaaattac ttctgccgct
     9421 gagcgtcatt gactgcgggt cgtaagagac gtcggtcaga tctgttaagc cctgagtgat
     9481 gtgacggctg gtactcagca cacgcggctc gttcggtcgc acatcgcgga cgcacaagac
     9541 gcgacagtct cccgggggaa tgtcagtctc aaacacctcg tccgtaaccg ccaggagttg
     9601 ctgctgccag aaatcgtaga cagccaagcg gctcctggca ggcggcgaga taccgaggcg
     9661 cttaaggccg atggtctctg tcttggcgac ggcggacggg ttgctcaaga gcagcacgtt
     9721 ccattgctca ggcgggcggc cgatggcgag gttccacgcc gtggtatcgc cgaagcgatc
     9781 gactcgacgg atcggctgga gcgggcaagt ccgccgaagg agttcgaatc ggtcaaccgg
     9841 aaggcggttc agggcctctg tcagccaggt catcgcggtt acttcggcag agggggcaac
     9901 ggcccactcg ctcgccaatc gccgcgcctc cttcagcgga accggcggcg ccagccgttc
     9961 gacaggaagc ggaggcctca aggaggtccc ttctccatgg tcggaaacct ggagatgaag
    10021 cctcaggaga acgacttttt tgttcatcgt agcgtcggtc cgggctttca gccgatagcc
    10081 gccgtaagat gactcgatgc tgacatctcc cgagtacgtg gcgtacgtac cgccggaacc
    10141 gtcatacaga ctgtcaaaca aaagcctcgt tgccgggccg attgccgtct gggttgcacg
    10201 gaagccctcc ggaggacgat atcgagccgg atgaacgtcg gccggcaaca cggccgtcag
    10261 actcagttgc ggcagcctgc ttctcgactc gacggcaacg gttacctgac ggccgttgtc
    10321 acgcgataca atctggcagg tcatttccgg ggcaccgcca gccattgcgg ccagagcatg
    10381 agagaccacc acgtcatcca ccagctcacc gacacccggg ctctgcgcca gaggccgggg
    10441 cacaatcatg tgctcaacgg agcggcggga cagatgtgcg gtcacacctg tgagatagag
    10501 aatgtcgcgg tcacccagac gaagggggac accgggcatg cttgactcag gttgagtggc
    10561 ggagggaatt atagcgggct gactgatggt ggccgacgac gcggcaggaa tgccagggcg
    10621 tagcccggca caacctgcga gcagggccgt cagaatcaat gaacccaggc gcaggactcc
    10681 tgagcttctt ttatcggtat gaaataccct taaggaacct gacaaccgat cgttacccat
    10741 cgtcttgctc acaacggcag gggccaattc gtgagtgatg tcttcacgcc ggccatcaac
    10801 cgcatcaacg attcctggct tgcttcagcc cgcgacagct cgccgacctg tcgcccttcg
    10861 cgaagcacga ttattcgtgt gctgagatta aggacctccg gcagctcgga ggagatcagc
    10921 aggatcgccg ccccttcacg agcgagttcg tcgatcaggg catgaatctc ggccttggcc
    10981 ccgacgtcga cgccccgcgt cggttcgtcg atcagcagaa tcgcgcagcg gcttgccagc
    11041 cacttagcca tggcgatctt ctgctgattg ccgcccgaaa gcgaccagac gggtgcatcg
    11101 atgtggggcg tgcgaacccg aagtcgatca aagtaacggc gggtcagggc tttttcgtag
    11161 cccgtgcgga cgaagccggc tctgcagagg cgattgagga tcggcaatga caggttggcc
    11221 cgaccgccca tggagagcac caggccctgg gtcttgcggt cctcgggcag aaagcccact
    11281 ccaagagcca tggcctcacg aggtgagcgg atgtggaccg gtcggccgtc gatcaacaca
    11341 cggccggtgg ccttcgggtc caagccgaac acggcctggg ccacttcact tcgccctgct
    11401 cccaccaagc ccgccagccc gacaacctcg ccggccctta ccgtcaggct gatattccgg
    11461 aatttacccg gtgaggacag gtcctcgatt cgcagccttt ccgaccccgg cctggagtcc
    11521 agatgcgcgg ggaagtattg ctccaacggc cggccgatca tccgctgaac gatcttgtcc
    11581 gtggtcatat cggaaacgtc ggcggtctcg atgtgttgcc cgtcgcgaag caccgttacc
    11641 cggtcgcaga ggcggaagac ctcctccagg cgatgcgaaa cgtagatcag cgtcacaccg
    11701 cgagccttga gcttatcgat caacgcgaac aatcgctcgg
//
LOCUS       DAOTPW010000178        11666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5239_length_11666_cov_5.727155, whole genome shotgun sequence.
ACCESSION   DAOTPW010000178 DAOTPW010000000
VERSION     DAOTPW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5239_length_11666_cov_5.727155"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="P5316_15255"
     CDS             <1..265
                     /locus_tag="P5316_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRR86364.1"
                     /translation="DVDGDGRTDIVFSCEQSRGKRGLMWMKYANSPAEQNWTAFDIAG
                     LSGTKFDLVQPVDLDGDGDLDVMTTEETERLGVIWYENPFSGS"
     gene            complement(297..1241)
                     /locus_tag="P5316_15260"
     CDS             complement(297..1241)
                     /locus_tag="P5316_15260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR86365.1"
                     /translation="MRIRLALAAAAVAACAVGFAAAQQMRRPDARTARGRTDFALPPP
                     SPVIEVLDADKDGIISAAEREGAVAALAKLDRNNDGDLTPDELLPAFAEGGPPDMSGM
                     RRPGRGPGGAPPGWRGESAEASPIPKDDAEKRILDVLTELNKKPVPGGMSVPMEDGRL
                     LRLLTEAIGAKHVVEIGTSVGYSGIWLGLALKATGGKLTTFEIDARRAAEARQNFKKA
                     GVDELITLVEGDAHETVATVKDNIDLLFIDADKEGYLDYLEKLLPLVRPGGLIVAHNI
                     KPGQGDPGYLKAVTTDPKLETLFLHSEGAGVGVTLKKR"
     gene            complement(1467..2063)
                     /locus_tag="P5316_15265"
     CDS             complement(1467..2063)
                     /locus_tag="P5316_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRR86366.1"
                     /translation="MKTKIAFMGALITVCLIGWAIAGQGKHGAVKGKGLSSDEPAKPM
                     FCGGHPPMPIMAVLDADKNGELSAAEIDGAKAALLTLDADGDGVLTAKELRPVPPEPP
                     KDGEKPQAGDHIMRLDANGDGYVTLEEFTAPMTAQMKEVFAKIDTSGDGNIDKDEAAA
                     APPPPPPPPPPGRGHGMGPGGHGGCRFGPPPPPPADAE"
     gene            2312..2950
                     /locus_tag="P5316_15270"
     CDS             2312..2950
                     /locus_tag="P5316_15270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR86367.1"
                     /translation="MTSEQKTTKSRIERVRAGDLQALVAAFEEYRPRLAKTVLFRLDQ
                     RLAGRLDPDDVLQEAYISAAQRFGHVEGDDESALFIWLRMIVLQTLAELHRRHLGAQK
                     RDAGREIPLHQGASSESTCLSMTRHLAAGVTSPSLALRRSEFCEQLHHALDQMDSIDR
                     EVLALRHFEELTNQEVAEVLGIQQKAASIRYVRALRRLKAILDELSGVNPWA"
     gene            2999..6061
                     /locus_tag="P5316_15275"
     CDS             2999..6061
                     /locus_tag="P5316_15275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRR86368.1"
                     /translation="MNENDSERDPLEMLAAEFTERFRRGEQPSAEEYALRCPEMAGDI
                     RELFSMIVRVEQAKTTGSGRSRSEAIGNQPAPRQLGDFRIVRELGRGGMGVVYEAEQE
                     SLGRRVAIKVLPSEYLSSQSRRERFEREARTAARLHHTNIVQIFGVGQHDGRPYFVMQ
                     YIRGVGLDAVFRSLATRNMIARSSDNQQAGPNLAGSDLVQVARELLDSRTMTDSTDRE
                     TKPAQSGPHAYPTAPTAQSSAYPLVSSVGAPAGHGGNGSSRSFFSGPMGYWNNLARLF
                     AQAADALSFAHRQGVLHRDVKPANLLLDADGMLWVTDFGLAKTEDFAELTKADDVVGT
                     LRYMAPEQHEHRCDERSDVYGLGVSLYELATLRPAFVEKGGGLKAILSEPLFPPRRLC
                     PTMPRDLETIILKATAREPEHRYSSAAELRDDLLRFVSDLPIQARRISAPERLLRWAR
                     RNPALATAAGLILVLLISIAGIFSTAYFKVHKANMVTRTQMERAQATADLAVAAIDAI
                     FEEFAPVGLAVSQEISLNDTEDGSLVVKVPPVLSPETAAFLRRMLSFYQELAQQEGNA
                     PSLLLKIAAAQRRVGDIYSLLGQYEAARTAYGESATQYERLRERGTDDDTLDIALARL
                     KIQLGQTFASEDEMPEAADQYETARQILQSKLDAGSGSQEVRFELARACYMLTNPLLA
                     AVPIHPIDRPAHGPPDWAGPGPRGLAHGSGRPPALLLPHGPPPPAPQDLIPPGPPPMT
                     QPPDRGDRHRDRDRNRLHLQQAIQLLTGLIAEKPRVPEYRHLLALCLRETPGPQRPAS
                     RPADNGPLEQAVAILEDLVAEYPHIPQYQFDLSETYAVQARWDGSTDRLERALGIIQS
                     LVTRWPNVPSYQFSLGRLHLRLWDQGMQAGQPDAALPNLQRAVELLVDLTKRYPSVPS
                     YAVAAAFSEMTLAQWRQAQSRHDEARILLESAVRRLDELTASEGPKPHIGFMAAQCYG
                     QLARVYRSLGKHNLSREAEQKSATLRPPHGPAPLREPEAERPRSGGGI"
     gene            complement(6070..6240)
                     /locus_tag="P5316_15280"
     CDS             complement(6070..6240)
                     /locus_tag="P5316_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86369.1"
                     /translation="MRVAFEVYRGTLASWQELFQQAADFASQDGPERVISISHSCDHS
                     DGAVTVWYGTPT"
     gene            complement(6416..6934)
                     /locus_tag="P5316_15285"
     CDS             complement(6416..6934)
                     /locus_tag="P5316_15285"
                     /inference="COORDINATES: protein motif:HMM:NF022048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86370.1"
                     /translation="MGILDAEGLRKDSGASSSRRQKTRSIQAILRFIHEDRGRLSLEF
                     LRRRPMGRARAYLLALPGVGPKTAACVLLFNLGKPALPVDTHVHRVSWRLGLIPDDAT
                     AEDAHEILEPACPPKQVYAFHVLLIRHGRQICRARKPRCGECVLADICPSAQLRTGGR
                     LLKLKQRGQIGV"
     gene            complement(7442..8365)
                     /locus_tag="P5316_15290"
     CDS             complement(7442..8365)
                     /locus_tag="P5316_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86371.1"
                     /translation="MREFCTWIVTVGMLAAAAPARGDAITFTLLTHADNKMLLPQPKG
                     LVGVSGDHLVRTSDDITGSTFNPQGAFTFNFMNPIGIWEPDYPPGYAEGIHSMTGTFT
                     LDMNLWTGGSASISSLAFDGYVAPGKYSAQRLVRPGDTAANGAYGLVDGQPNSGSYAA
                     SADANWGFELLFDWFFDTPYAGSGTIDMTFDNYRWNGFIIPVSQLTPAGMDLVTLDDP
                     LGFFGGTSADFESWLLNEVQPRLPADATWLLFAQGTANPAWTNPGMGMTTDGLVDQTV
                     LAYTTIPEPAIAAGFLAGTVCLLANKWRHKR"
     gene            complement(8397..9002)
                     /gene="tsaA"
                     /locus_tag="P5316_15295"
     CDS             complement(8397..9002)
                     /gene="tsaA"
                     /locus_tag="P5316_15295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRR86372.1"
                     /translation="MPQDLQADSRSAVATAFTVRPIGYVHSTYSCPEDVPHSHRAWTE
                     DACRIRLLRRHANGLRGLEGYSHVIVLFWVHRAREWKMPRHHHKPPHVKVFATRMPVR
                     PNPIGMSVVELVEFNPERGDLVVRGLDALDATPVLDIKPYIPNFDSRPDARLPRWVAE
                     HLASHFHGGHEHKGATPGNDPGCERHDRNLKARARARKSSI"
     gene            complement(9008..9610)
                     /locus_tag="P5316_15300"
     CDS             complement(9008..9610)
                     /locus_tag="P5316_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86373.1"
                     /translation="MRTTALVPTGWLASQGSHVRVIAILRDLLLVAVAGLLCAWIGQL
                     LGRHVRVGLAMPLVGSAAAAMPRALILLMVLWRVNRTGVLTAAAVAEVLTRLTVGVPG
                     LLPTGLVVPVLAATVADAVWRGTSGWHESRLRLAVTGAVLAGTRVAAAWLLLALVTLP
                     PGMRMPGTATCCAVLVVNTLLGIGAGIVVARPWRHRSGTR"
     gene            complement(9607..10338)
                     /locus_tag="P5316_15305"
     CDS             complement(9607..10338)
                     /locus_tag="P5316_15305"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HRR86374.1"
                     /translation="MGLIMRCRWLQSAGRALLLPARSGAEGLDQLGHAGVTLLELLVV
                     VSITGIMVGLLLPCLGGAKEVARRTVCATNLRSLGQGLHLYALNHDGRFPVEELCGNP
                     QGSLVDSLYPLHVPARGVFYCPSADQIEPYAQSHEYGGPGGDSVVNTDENWQRRWITY
                     KYFSITRRDPRMPLPLRASEYPHLLRSDSTAQRWLMSDWVRQDVPVFPHRQKGGWGGG
                     RNVLFVDGSVQFVNHRTPGAFGDKR"
     gene            complement(10858..11541)
                     /locus_tag="P5316_15310"
     CDS             complement(10858..11541)
                     /locus_tag="P5316_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR86375.1"
                     /translation="MPRQARVAPGGLIYHVLNRAVARLPLLQKPRDYEAFESVVAEAW
                     QRCPIRLLAYCLMPNHWHMVLWPRADGELTAFVRWLTHAHTMRWHTHYHNTGTDHLYQ
                     GRFKSFPVQSDEHFLTVCRYVERNALRANLVERAEDWRWSSLWRRHCGVPEAREILSD
                     WPGGIPRNWLATVNRPQSQAEVDAIRVAVRRGQPLGSPAWITRTAGQLGLESTLRPRG
                     RPRKQDHDS"
BASE COUNT         2171 a         3756 c         3554 g         2185 t
ORIGIN      
        1 ggacgtcgac ggcgacggtc ggaccgacat cgttttcagt tgtgagcaat caagaggcaa
       61 acgcggcctg atgtggatga agtacgccaa ctcgcctgcc gagcaaaact ggaccgcctt
      121 tgacatcgcg ggtctcagtg gcaccaagtt cgaccttgtc caaccggttg acctggacgg
      181 cgacggcgac ctcgatgtca tgaccaccga ggagaccgaa aggctgggcg ttatctggta
      241 cgagaacccg ttctcgggtt cctgatgttt tcggcaagcc gtcgacaacc ggtctgtcag
      301 cgcttcttga gagtcacgcc gactcccgcg ccttcactgt gaaggaacag cgtttccagt
      361 ttcgggtcgg tggtaacggc tttgaggtag cctgggtcac cctggccggg cttgatgttg
      421 tgagcgacga tcaacccgcc gggtcgaacg agcggcagaa gcttttcgag atagtccaga
      481 tagccttcct tgtccgcatc gatgaaaagc agatcgatgt tgtccttcac ggtcgcgacc
      541 gtctcatgag catcaccttc tacgagcgtg atcaactcgt cgactcctgc tttcttgaag
      601 ttctgccgcg cctcggccgc acgtcgggcg tcgatctcga aggtggtaag cttgccgccc
      661 gtcgccttca aggccaggcc gagccagatt cccgagtaac caaccgaggt tccgatctcg
      721 accacgtgct tggctccgat agcctcggtc agcaggcgga gcaaacggcc atcttccatg
      781 ggtacgctca tgcctcccgg caccggcttc ttgttgagtt cggtcaacac gtcgaggatt
      841 ctcttctcgg cgtcgtcctt gggtatcggt gatgcctcgg cactctcgcc acgccacccc
      901 ggtggcgcgc cgccgggccc tcgaccgggg cgtctcatcc cgctcatatc cggcgggccg
      961 ccttcggcga acgccggcag aagttcgtcg ggcgtcagat ctccgtcgtt gttacgatcg
     1021 agcttggcca gcgcggcaac ggctccttcc ctttccgcgg ccgagataat cccgtccttg
     1081 tccgcatcca gtacctcgat cacagggctc ggcggcggta gcgcgaagtc cgttctgccg
     1141 cgtgccgtcc gcgcatccgg ccttctcatc tgctgggccg ccgcgaagcc caccgcgcaa
     1201 gccgccaccg ccgcggctgc aagagccaat ctgattctca ttgttgagac tccttcattc
     1261 ttgtctgact tgccttcctg cggcggggtt gccccgcgtc accccaatgc gtgtgcatcc
     1321 tgcaagtcgg tctgtttgcc gcaagcccgc caatgcgcgg accatcgcac attgtaaacc
     1381 gatgccgtct gccccgtaac aggagcagat cggcatcggc cttcctgtgt tctcaaagct
     1441 gcggtccctc cgcggcccgc ttttcgttac tcagcatccg caggcggcgg tggaggaggt
     1501 ccaaatcggc agccgccatg gccaccaggc cccatcccgt gacctcgccc cggaggagga
     1561 ggaggaggag gcggcggcgg agctgccgcg gcttcatcct tgtcgatgtt cccatcgccg
     1621 ctcgtgtcaa tctttgcgaa gacctccttc atctgtgccg tcatcggcgc cgtgaattcc
     1681 tccagggtca catagccgtc tccattcgca tccaagcgca tgatgtgatc accggcctgg
     1741 ggtttctcgc cgtcctttgg gggttccggg ggcaccggtc gcagttcctt ggccgtcaac
     1801 actccgtcgc cgtcggcatc caacgtcagc aatgccgctt tcgcgccatc aatttcggct
     1861 gccgagagtt caccgttctt gtctgcgtcc aagaccgcca taatgggcat aggcggatga
     1921 ccgccacaga acattggttt ggctggttca tcgctcgata agcccttgcc cttcacggcc
     1981 ccgtgcttgc cctgtccggc gatggcccag ccgatcaggc agacggtaat cagtgctccc
     2041 ataaacgcga ttttggtctt cattgctgct tctccgtttt ggattcctgc tcttgcccca
     2101 cggacgctcc gcgcggctat tcatggaaac gcaaaaggta aggctttttc tacgggtgct
     2161 cctatgaaaa tcgcccctga tcgcaacttc cgacgctccc aggcgttaca atttgtggag
     2221 aaacgccggt tgggcaagtg cgactcggtc catatgaagc gagggcaccg acctgtctct
     2281 ctatacgggc ccgtctgagg ggcaagcagg tatgaccagc gagcagaaga caacgaaaag
     2341 ccgtatcgag cgagtacgag ccggagacct tcaagctctc gttgcggcat tcgaggagta
     2401 tcgcccccga ctggccaaga cggttctctt ccggttggat cagcgccttg ccggacggct
     2461 ggaccccgat gacgttctcc aggaagccta catcagcgct gcccagcgtt ttgggcatgt
     2521 cgaaggcgat gatgaatcgg cgctgttcat ttggcttcgg atgattgtcc tacagacgct
     2581 cgccgagctt caccggcgac acctcggcgc gcaaaagcgc gacgccggcc gcgaaatccc
     2641 cctgcatcaa ggggcatcct ccgagtcaac ctgtctgtcg atgaccaggc atctggcggc
     2701 cggcgtgacc tcgccttccc tcgcgttgcg ccggtcggag ttttgtgaac aactgcatca
     2761 tgctctcgat cagatggact ccatcgatcg ggaagtgctg gcattgcgtc attttgagga
     2821 gctcacgaac caggaggtcg cggaagtact gggcattcaa caaaaggccg cgagcattcg
     2881 atacgttcgc gccttgcgcc ggctcaaagc cattttggac gaattgtccg gcgtgaatcc
     2941 ttgggcctga tgcggcgacc ttcgacgagg ccggcgcgcg agacgacgga accgaattat
     3001 gaacgagaac gatagcgaac gagatcccct ggagatgctc gcggccgaat tcaccgagcg
     3061 gtttcggcgc ggcgagcagc cctctgcgga agaatacgcc ttgcggtgtc cggagatggc
     3121 cggtgacatc cgtgagctgt tctccatgat tgtccgcgtc gagcaggcca agacgaccgg
     3181 ctccggacga tctcgctccg aagcgatcgg caaccaaccg gcgccgcgac agctcgggga
     3241 ctttcgtatt gtccgcgaac tcggtcgtgg cggaatgggg gtcgtctatg aggctgagca
     3301 ggaatcgctt ggacgacgcg tggccatcaa ggttcttccg tcggagtacc tgtccagcca
     3361 atctcgccgc gagcgttttg aacgcgaggc gagaaccgcc gcacggttgc accacaccaa
     3421 tatcgtccag atcttcggcg tgggacaaca cgacggccgt ccctatttcg tgatgcagta
     3481 tatccgcggc gtcgggctcg atgcggtttt ccggagtctg gccactcgga acatgatcgc
     3541 ccgctcaagc gataatcagc aagccggtcc gaacctggcc ggctcggacc ttgtccaggt
     3601 ggcgagggag ctgctcgact cgcggaccat gacggattcg acagaccgtg agacgaaacc
     3661 ggcacagagc ggcccgcatg catatccgac cgcgccgacg gcccaatcat cggcataccc
     3721 gctcgtctcg tcggtcggtg cgcctgccgg acatggcggc aacggctcgt cgcgttcgtt
     3781 cttttccggc ccgatgggct actggaacaa cctcgcccga ctcttcgccc aggcggccga
     3841 tgcgctttcg tttgcgcacc gacaaggcgt ccttcaccgc gacgtcaaac ctgcaaacct
     3901 cctgctcgac gcggacggca tgttatgggt gaccgacttc ggcctggcca agaccgagga
     3961 tttcgccgag ctcacaaagg cggatgacgt cgtgggcaca ctgagataca tggcccccga
     4021 acaacacgaa caccgctgtg acgagcgcag cgacgtctac gggctgggcg tcagtctcta
     4081 tgaattggcg acgcttcgac cggcgtttgt cgaaaaaggc ggagggctca aggcgattct
     4141 ttcagaaccg ctgtttccgc cgcgccggct gtgcccgacc atgcctcgag acctggagac
     4201 gatcattctc aaagccaccg cccgcgagcc ggagcaccgc tattcgtcgg ccgccgagtt
     4261 gcgagatgat cttcttcgat ttgtcagcga tcttccgatc caagcccggc ggatctcggc
     4321 acccgaaagg ctgcttcgct gggcccggcg caacccggct ctggcaaccg cggccggcct
     4381 gatactcgtt cttctgatct ccatcgccgg gatcttctcg acggcctatt tcaaggtcca
     4441 caaggccaat atggtcactc gcacgcaaat ggagcgggcc caggctacgg ccgatctggc
     4501 cgtcgccgcc atcgacgcca tcttcgagga gttcgcgccg gtcgggctcg ccgtctcgca
     4561 ggagatcagc ttgaatgaca cggaagatgg cagcctggtc gtgaaggttc cgcccgtgct
     4621 ttctcccgag acggccgcat tcctgagaag aatgctgtcg ttctatcagg agcttgcaca
     4681 gcaggagggc aatgccccat cgcttcttct caagatcgcg gccgctcaac ggcgcgtggg
     4741 tgacatctac agcttgctcg gccaatatga agctgcgaga acggcgtacg gggagtcagc
     4801 cacacagtac gaacggctcc gcgagagggg aaccgacgac gacactctgg acatcgctct
     4861 ggcacggctc aagattcaac tgggacagac attcgcctcc gaggacgaaa tgccggaagc
     4921 cgcggatcag tacgagacgg ctcggcagat cctgcaaagc aagctcgacg ccgggtccgg
     4981 ctcgcaggag gtgcggtttg agcttgccag agcctgctac atgctcacca accctctatt
     5041 ggcggccgtg ccgattcacc ctatcgaccg tcccgcgcat gggccccctg attgggccgg
     5101 gccgggtcct cgtggcctgg ctcacgggtc cggccggcct cccgcactgc tactgccgca
     5161 cggaccgcct ccgcccgccc cccaagattt gatcccgccg ggaccgccgc cgatgaccca
     5221 gccgccggat cgaggagacc ggcaccggga tcgcgaccgg aaccgcctgc atttgcagca
     5281 ggctatccaa ttgctcactg gactgatcgc ggagaaaccc agagttcctg aataccgaca
     5341 tctgttggcc ctgtgccttc gcgaaacccc cggcccgcag cgcccggctt cgcggcccgc
     5401 cgataacggg cctttggaac aagcagtggc catactggaa gacctggtcg ccgagtaccc
     5461 gcacataccc caataccagt tcgatttgag tgaaacctac gccgtgcagg cgcgatggga
     5521 cggctcgaca gatcggctgg aaagggctct gggcatcatc cagtcactcg ttacccggtg
     5581 gccaaacgta ccaagctacc agttttcgct cggccggtta cacctgcgat tgtgggatca
     5641 aggcatgcag gcgggtcagc ctgatgccgc cttgcccaac ctgcaacggg ccgtcgagct
     5701 gctcgtcgat ctgacgaaac ggtatccgtc ggtaccgagt tatgccgtgg ccgcggcgtt
     5761 ctccgaaatg accctggccc agtggcggca agcccagtcc aggcacgatg aggcccggat
     5821 cctgctggaa tccgcggtgc gtcgcctgga cgagttgacg gcctccgaag ggcccaaacc
     5881 acacattgga ttcatggccg cccagtgcta cgggcaactc gctcgcgtct accgcagctt
     5941 gggcaagcat aatctgtcgc gcgaggccga gcagaaatcg gcgacccttc gcccgcccca
     6001 cggccctgca ccccttcggg agcctgaggc agagcggcca agatccggcg gcgggatctg
     6061 atcaggccgc tacgtgggcg tcccgtacca gacggtcacc gccccgtcac tgtgatcgca
     6121 ggagtgcgaa atcgagatga ctcgttcggg gccgtcctga ctggcgaaat cagcagcctg
     6181 ttgaaacagc tcctgccaac tggccagcgt ccctcgataa acctcaaaag caactcgcat
     6241 cagatcatct cccattcatt cctgctcgaa atgtgtgtga aaaccgtagg gcatggtcgg
     6301 agccaccgaa ggtggcggag acctgccggt cttggcgctc ccctacctcc acatctgctg
     6361 gccccagatg cgtgagcagg tggggcaccc gagaggtttc ggttctcgca cacatttaga
     6421 cgcctatttg acctcgttgt ttcaacttca gcagcctgcc tcccgtcctc aactgagccg
     6481 acgggcagat gtcggccagc acgcactcgc cgcagcgcgg ctttcgggcc ctgcagatct
     6541 ggcgaccgtg gcggatcaga aggacgtgaa atgcatatac ctgcttcggc gggcaggccg
     6601 gttcgagaat ttcgtgggca tcctcggccg ttgcgtcgtc ggggatcagc ccaagccgcc
     6661 aggaaacacg atgcacgtgt gtgtcgaccg gcaacgcagg cttgccgagg ttgaagagca
     6721 atacgcacgc cgcggtcttc ggcccgacgc ccggcagggc cagcaggtac gcccgggctc
     6781 gtcccatcgg ccggcggcga aggaattcga gcgacaaccg gccgcgatcc tcgtggatga
     6841 accgcaagat cgcctggatg ctgcgcgtct tctgtctgcg tgagctgctg gccccagaat
     6901 cctttctgag gccttccgcg tcgaggattc ccatcctcat tatcaccgaa tgccgaacgg
     6961 attcggccag gggaagccgc ctgggaggta ttacgagcct gccggcccca gaatcctctg
     7021 ggggcctccg tgtcgagggg ctgaccgccc cgcccgtcgg caggattggc cacgaacagt
     7081 cgcaagaagg cacaaaaagg gaaggggagc gatcgggggg ctgtcaggct gttcgactgt
     7141 tcggctcttg ggggccgggc aaaggaaccg gtcggcggga aaggttggac tcctcgccct
     7201 tgcggttcct gcctcccaac agtctaacgg actaacagcc tgacagtcca aggcggcctc
     7261 cagccgccct tgcatcccac aacctgcggt ccagtcttcc ggtccgtttt tgagggccgg
     7321 ctttccagtc cttgaacccc acaggcggcg actcgggctg gtaatctgga tcccacgcag
     7381 acgccttggc gcgaggctga cgggcacccc cagatcggcg atgaacaacc gatagcagcg
     7441 gctaccgctt atgtctccac ttgtttgcca gcaaacagac tgtccctgcc aagaaccccg
     7501 ccgcaatcgc tggctccggg atggtagtgt aggccaacac ggtttgatcc acaagcccat
     7561 ccgtcgtcat gcccattccc gggttggtcc aggcagggtt agcggtaccc tgggcaaaca
     7621 gcagccaggt tgcatccgcc gggagcctgg gctgaacctc gttcaggagc catgattcga
     7681 agtccgccga agtgccgcca aagaatccaa gggggtcgtc cagcgtgacc aggtccatcc
     7741 ccgccggcgt cagttggcta accggaatga tgaaaccgtt ccagcggtag ttgtcgaagg
     7801 tcatgtcgat ggtgcccgag ccggcatagg gggtatcgaa aaaccagtca aagaggagtt
     7861 caaaccccca gttcgcgtcg gctgaggcgg cataggaacc gctgttaggc tgaccatcca
     7921 cgagaccgta ggcgccgttg gcggccgtgt cgccggggcg caccaggcgc tgcgcgctgt
     7981 acttgcccgg cgcgacgtat ccgtcgaagg cgagtgacga gatggaggcg gaaccgccgg
     8041 tccacaggtt catgtcgaga gtgaacgtcc cggtcatgga atgaatgccc tcggcgtaac
     8101 ccggcgggta gtccggctcc cagatgccta tcgggttcat gaagttgaac gtgaaggctc
     8161 cctgggggtt aaaggtgctc ccggtgatgt cgtcggacgt tctgaccaga tggtcgcccg
     8221 atacacccac caggcccttc ggctgaggta ggagcatctt gttgtccgcg tgagtcagca
     8281 gggtgaacgt gattgcatcg ccccttgcgg gggcggcagc cgccagcatt ccaaccgtaa
     8341 cgatccaagt acagaattct cgcatgtttg ttcctccaag attcccgttg ctttaatcaa
     8401 atgcttgact tccgcgccct tgcgcgagcc ttgaggtttc ggtcatgtcg ctcgcagccc
     8461 gggtcgtttc ctggtgtcgc ccctttgtgt tcgtgccctc cgtggaagtg gctcgccaaa
     8521 tgctcggcaa cccagcgcgg taaccgggcg tccggccgac tgtcgaagtt gggaatgtag
     8581 ggtttgatgt ccagcactgg cgtggcgtcc agcgcatcca gcccccgcac gacaaggtcg
     8641 ccgcgctccg ggttgaactc gaccagttcc accacggaca tcccgatagg attcggccga
     8701 acaggcatcc gcgtggcaaa caccttcaca tggggcggct tgtgatgatg cctgggcatc
     8761 ttccattccc tggcacggtg gacccagaac agcacaatga cgtgggagta accttccagg
     8821 ccccgcaggc cattggcatg ccggcgaagc aatcggatcc gacacgcgtc ctccgtccag
     8881 gcccgatgcg aatgagggac atcctccggg caggagtagg ttgaatgcac gtagccgatg
     8941 ggtcgcacgg taaaagccgt ggccactgcg gaccggctgt ctgcttgcag atcttggggc
     9001 atcgctctta cctggttccg ctacggtgac gccacggccg tgcaaccact attccagccc
     9061 ctatgccaag cagggtgttc accactagga ccgcgcagca tgtcgcggtt ccgggcatgc
     9121 gcatccccgg cggcaacgtt accaaggcca acagcagcca ggcggcagct accctcgtcc
     9181 cagcgaggac ggcgcccgtg acggccaggc gcagccggga ctcatgccag ccgctcgttc
     9241 cccgccaaac agcgtcggcg acggtcgccg ccaggacagg cactaccaac cctgttggca
     9301 acaggcccgg cactcccacg gtgagccgtg tgaggacttc cgcaaccgcg gcggccgtca
     9361 gaacgcctgt gcggtttacc cgccacaaca ccatcaacag gatcagcgcc cttggcatcg
     9421 ccgccgctgc ggaacccacc agcggcatcg cgagccctac tcgcacgtgc cggccgagaa
     9481 gctgaccgat ccatgcgcac aggagccccg caaccgctac cagcaggagg tcgcgaagga
     9541 tcgcgattac ccggacatgc gacccttgtg aagccaacca gcctgtcgga accagtgctg
     9601 tcgttctcac cttttgtctc caaatgcccc cggtgtcctg tggttcacaa actgaacgct
     9661 tccgtccacg aaaagcacgt tccgtccgcc gccccagccg cccttctgcc ggtgcgggaa
     9721 cacgggcacg tcctgcctca cccagtcgct catcagccac cgctgggctg tagagtcgct
     9781 gcgcaagagg tgtgggtatt ccgacgcccg caaaggcaag ggcatgcgcg gatcgcgccg
     9841 tgttatcgag aagtacttgt aggtgatcca gcggcgctgc caattctcgt ccgtattcac
     9901 cacggagtca ccgcccgggc cgccatactc gtggctctgc gcgtacggtt cgatctggtc
     9961 cgcgctgggg cagtagaaga caccgcgggc gggtacatgg agcgggtaca ggctatccac
    10021 cagcgacccc tgcgggttgc cgcagagttc ctctacgggg aaccgtccgt cgtggttcag
    10081 ggcgtagagg tgcaatccct ggccgaggct gcgcaggtta gttgcgcaga ccgtcctcct
    10141 ggctacctct ttggcccccc ctaggcaggg cagcaggagc ccaaccatga tgccggtgat
    10201 gctgaccacg accagcagct ccaacagggt cacgcccgca tgccccagtt ggtcgagacc
    10261 ttcggccccc gagcgggcag gcaggagcaa tgcacgacca gcggattgta gccagcggca
    10321 cctcataatc agccccatcg tgttacgatc cgcagaatag tattaccaaa gacggcaagc
    10381 cgttgcaaga ggaaaacctg cgggaattct cggccacacc catcgttctc agcagaaaga
    10441 gcggaccacc gttcttgggg tttcgtcctg ccggtacctg cgggacgtcc aagtgcttta
    10501 tcggcgcgcg agcgaggcgg acaaggcgag catcattgac accgcagccg acacgtcgga
    10561 gttgacgtcg ctggtgtgct gcgacaggac ggttcaatca gcccgtccag gggccgaccc
    10621 cacgaccgcc atcgctgcgg accatgcacc ttctcgagac ggcggacgat gaggcgaatc
    10681 ttcgatcgga tactggtgcc cgttcgcatc gtcatcctac cttgacgagc cgaggggttt
    10741 atcccctcgg tcctgacgag ccggcacgga taaggggacg ggcacggata aggggcacgg
    10801 ataaggggac gcacggataa ggggacgcag ctcttttgtc aaccctccta atgcttctta
    10861 actgtcgtga tcctgctttc tgggccgtcc gcgaggtcgg agggttgatt cgaggccgag
    10921 ttgcccggcc gtgcgggtga tccaggcggg ggagccgagt ggttgacctc gccggacggc
    10981 cacgcgaatg gcatcgacct cggcctgact ctgcggccgg ttgaccgtcg ccagccagtt
    11041 ccgcgggatg cctcccggcc agtcggagag gatctcgcgg gcctcgggca cgccgcaatg
    11101 acgccgccag aggctggacc accgccagtc ctcggcccgc tcgaccaggt tggcccgcag
    11161 ggcattccgc tcgacatagc ggcagacggt caggaagtgc tcgtcgcttt gcacggggaa
    11221 cgacttgaaa cgcccctggt aaagatggtc ggtccccgtg ttatgatagt gtgtgtgcca
    11281 gcgcatggtg tgcgcgtgcg tgagccagcg gacgaaggcg gtcagctcgc cgtcggcccg
    11341 cggccacagc accatgtgcc aatgattggg catcaggcag taggccagca gacggatcgg
    11401 gcagcgctgc caggcctcgg ccaccacgga ttcgaatgct tcatagtccc gcggtttctg
    11461 gagcagcggc aaacgggcta ccgcccgatt caggacgtgg tagatcaagc cgccgggagc
    11521 aacacgggct tgtcgaggca tgggctgaag ggtagcatag aggcatatgg aagttaagaa
    11581 aaagagctgc gtccccttat ccggtctgag acgatggcat cagcgcgatc ctattgggta
    11641 tgtcgacggc gcaagcctct acgagt
//
LOCUS       DAOTPW010000179        11555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5294_length_11555_cov_5.601043, whole genome shotgun sequence.
ACCESSION   DAOTPW010000179 DAOTPW010000000
VERSION     DAOTPW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5294_length_11555_cov_5.601043"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..1332)
                     /locus_tag="P5316_15315"
     CDS             complement(286..1332)
                     /locus_tag="P5316_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRR86376.1"
                     /translation="MDPQVTYVLIIVAIALLFDFSNGFNDAANSVATIVATRVLTPRK
                     AVLWAAFFNFVAAFGFGTPVARTIGNGLVQAGVVDSHLIMATLIAAIVWTVICTRHGM
                     PISVSHALIGAMSGAAIVKGGFHALIWSTIGKIAVFIVLSPLIGLVIGWVMMVATMWA
                     GTWVSQAFKGITPRGIDRVFRVLQLFSAAVYSLSHGLNDAQKTMGIIFALLMSVPALT
                     GWATHGGEGGHEIAWWIILSCHAAIASGTYIGGWKVVKTLGHRVTRLQPMGGFCAETG
                     GGVTILALSHFGIPVSTTHTITGAIFGVGITTRKGGVRWSVGTEIVTAWVLTLPVSAT
                     FAALVYFAISRFVP"
     gene            complement(1363..1986)
                     /locus_tag="P5316_15320"
     CDS             complement(1363..1986)
                     /locus_tag="P5316_15320"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRR86377.1"
                     /translation="MFSVIPRDTKFFELFEASATVMIRAAQAYAELGRDYARREEIIQ
                     RIRGYEHEGDEVTHRTLDTLDKTFITPFDREDIQRLIVEMDDVVDEIDAAAKRLVLYR
                     IDTPTPWLVKQTEVLGKATALIGQALPKLRNLKKPGQVHEYLVEIHHLENVGDDTNHA
                     AVADLYNHCKDPIYAMKWKEIYDITERAIDTCEDVANTIEGIILKNT"
     gene            2527..3147
                     /locus_tag="P5316_15325"
     CDS             2527..3147
                     /locus_tag="P5316_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86378.1"
                     /translation="MEALLSHNMREKGAPTLNHAAGFGRRGDPDAALPNAGGGLRTCS
                     EHLRARESGGRSLRGMLIGLLAVGLASGCSNWPSGTSNKSGGLKQLNPRGVAFLEEIP
                     VPDGFQLVDEHSMDHESAGQRMARHEYKGRADPFAVRDFYRQRMPQMGWERVSDQSVK
                     GVITMRFEKKLEACTIQIRPGHFGQSIVNVEVVPFSRTPMEPPKQR"
     gene            3144..3914
                     /locus_tag="P5316_15330"
     CDS             3144..3914
                     /locus_tag="P5316_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86379.1"
                     /translation="MKQRRRQELKTNELSIYLGQMYDFLVRNSTYVVGGVVVVALVII
                     VATMNARSQHRAKVDAMNRIRAIQSGEAVQDLKLLDEIKDLIAEYGDDPWLGPSLLDL
                     QASLAHHLALGRTSEADKAEHDRLLDEARSACERAIQRFRSQPLVMARAKLALAAVEE
                     TLFVEGKGDQERIRKLYQEVIDGPVSPYQRLASEQLSSLDARLAKLEIVATRPAAGAP
                     TSASAITLPPIALPTAPATATIAPATEPAAAPVGKPTE"
     gene            3939..5981
                     /locus_tag="P5316_15335"
     CDS             3939..5981
                     /locus_tag="P5316_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRR86380.1"
                     /translation="MRWHCLLAVITVLTSVLPLCADEGMWLLTRPPTEVLKSKHGFEP
                     TRAWLEHLQKSCVRMGASGSLVSADGLLMTNHHVGVDDLYKLSTPQRDLVRDGFYART
                     HDQELRCPDLEVQVLWSIEDVTEQVAAAATAEMPPGEAYEARRKAMTRIEQESKDATG
                     LDSEVVTLWYGARYHLYRYRRYTDMRLVFAPEKSVAFFGGDTDNFEYPRFDLDCCFFR
                     IYENDKPLKPEHYLKFSRRGAADGELLFVLGHPARSRRLVTVEHLRFLRDVEMPAVLN
                     WLWRREVQLAVFCNRSDENLRIGENDYFSVQNSRKARTGMQQAMLDPRFILQKEQAEA
                     TLRAAAKTTEPWDRIAEAQRVYRTFFNRRNTLREGHPLESKLFEIARTLVRLADELPK
                     PSPERLREYRESELESVYLWLYSPAPIYDELEIEGVTSGLTLMAERFGADDPLVVKAL
                     DGLSPQARAERLVRDCTLKDVETRKRYVVEGKKAIDASEDPMIRFAAAIDPESRALRK
                     RFEDEVEGVERENYARIGTAQFALYGEDLYPDATGTLRFAFGVVKGYEQNGRQIPPFT
                     DFAGLYERWKERGPRPPFDLPQRWLDRRDRLDLKLPLNFVLTADIIGGNSGSPIVNAK
                     GEVVGLIFDGNLQSLVWDIAFDDRQGRAIAVDTRAIIEALRKIYDADELADELMRE"
     gene            6070..7779
                     /locus_tag="P5316_15340"
     CDS             6070..7779
                     /locus_tag="P5316_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRR86381.1"
                     /translation="MPRPLADVPAGSPKAYLEGKKTRPSAGPRRLIGEPATAGDREPR
                     GYGGKPFFCYDSARPFVNPWFKSTSMSNFTWIDGSIVGVYLLATMAAGIAVRRYVGKV
                     EHFLVAGREMNLYLGIASLAATEFGIVTCMYTAQLGYEAGFAGATPGILYAAAMLLVG
                     LTGFCIKPLRDAGVITIPELFQNRYGSRVRWLSGVVIVLGGLLNMGVFLRMGGDFLVQ
                     VCGLEVRHLVPMMGMLLAAVAIYTILGGMLSVLVTDFLQFIVMSAGLLIVTLLTLHNV
                     GWSKLVETVNTHYGAGGFNPLVHPKMGIDYVVYNGVVNLAAVLTWQTIIQRLLAAKDT
                     RTGRRIYTHTSFFFVCRFLIPAIWGIAALSIMAPDQLAGIFPEVDQVRRSLLAMPRYL
                     STAVPWGLMGLLVAAMLAADMSTDSAYMLTWGGVIYNDILAPFRRNWTERKGILINRA
                     IIASIGVFLFFFGLYYDLKGDVWTYLTVTGSIYLSSMSVLLIACCYWKRANSWGAMAA
                     IVVGAIVPITLLVLQQTDRYKPFCDEWQYVLGAMTYATAGAAMIIGSLLKPRGTVTSG
                     AGE"
     gene            7786..7938
                     /locus_tag="P5316_15345"
     CDS             7786..7938
                     /locus_tag="P5316_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86382.1"
                     /translation="MREHWFWWLLTAAVVTWYSTVTVYVAIKGVTDIRTMLRHLGSVK
                     STEGES"
     gene            complement(8093..8644)
                     /locus_tag="P5316_15350"
     CDS             complement(8093..8644)
                     /locus_tag="P5316_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRR86383.1"
                     /translation="MDVHDAIRRRRSVRAYLPKPIPDDVMERLKQAIRFAPSACNYQP
                     WHFIIVKDAHLREEIVRQSHKQKWMLDAPVFVVACGFPLQAYKAMGGYGNSADVDVAI
                     ALDHLTLAAVSEGLGTCWIGAFDEASVKKLLDIPAQAKVVAMITLGYPASPNLNYPLD
                     ESCRKPVSEIFSLDRYPAGSRTF"
     gene            complement(8744..9496)
                     /locus_tag="P5316_15355"
     CDS             complement(8744..9496)
                     /locus_tag="P5316_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86384.1"
                     /translation="MDKTPPSRYQRFLRWAAARRLVIPRDTRVDPSLLSEERFPVSCP
                     KCEYLLRGLPDGRCPECGREFDHGKLLVEQYVGEAGKRSHPVTSKWTFRMWLAGALLL
                     VCGGMLPSLMQWVEQRAAGLRQTPGSDRLLRACVFTMIGAQLVGLSLLAVAFVLDLRL
                     AIVSRRKRRQILDAIDRNQPGFVEAQRAIWVTPALLLGLGAAFGTWYLLLPVFGQSGT
                     AMHPLHLVLSIVIGCGTTIGIVLAAGCVRGRK"
     gene            complement(9630..11126)
                     /locus_tag="P5316_15360"
     CDS             complement(9630..11126)
                     /locus_tag="P5316_15360"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRR86385.1"
                     /translation="MATISGLLPEVQAFLLQDPIPMFVGGRWIEAADGGVLQTIDPGD
                     GSPLATVAEGKAADVDKAVQAAREAFRKSGWATMPANERAVFLHRLADLVDQNRATLA
                     QLESLDVGKPLSQAAGFDVPNVAQTLRWYADLMVRTRRSELIAVSGFEARQVRFPYGV
                     AAFILPWNFPLLLVGWNLSPALAAGNTAVIKPAEDTPLSTLYFCKLVEQAGIPAGVVN
                     VVPGYGHTAGKALAEHPGINRMGFTGSPEVGRLIASACGNNLVPVKLELGGKGAAVVF
                     DDVDPAVVAQKLVAAVTLNTGQVCCTATRWLIHEKIWNPFVKIAREAMQQIKIGHGLA
                     PSTQMGPAVSEKQRQRILGYLEKGKAQGAEFVLSGGPATVTEHPEGFYVKPCMLTGDP
                     DNVCAREEIFGPVSFLMKFSEEEEAVELVNRSNYGLANSVWSADLKRANRVAEALVAG
                     NSWINGHNLFPHGVPYAGCNLSGCGGGVLGPETLFDYLRPQSVVRALS"
BASE COUNT         2333 a         3685 c         3430 g         2107 t
ORIGIN      
        1 aaccgagatc ttaagtcgtt atcgattaaa cacttaagga aaaccgtggc cggtttcccg
       61 tcatccgatc catcccgaac acgtcgccca acctcccaac ccgccttcca cgactgcgcc
      121 gccctccctc cccggacccg gaaaaccggg tccaccccgc cctgccaaca gcctcagttc
      181 cctcggcaac gaccattccc atcgccccca ccgccgacaa cccagaccat gaattcatcg
      241 gggacggtac cattccaagt cattcccgtc ccgcggccac aagactcagg gcacaaaccg
      301 tgagatggcg aaataaacca gcgccgcaaa cgtcgcggac accggtagag tcagcaccca
      361 ggcagtcaca atctccgtgc caacgctcca ccggacccca cctttgcggg tcgtgatgcc
      421 gacgccgaag atggcgccgg ttatggtgtg ggtggtgctg acgggtatgc cgaaatgcga
      481 aagcgccagg atagtcaccc ctccgccggt ctcggcacag aatcccccca tcggctgaag
      541 cctggtgacg cggtggccga gcgtcttgac gaccttccat ccaccgatat aggtccccga
      601 tgcgatcgcc gcatggcagc tcaggataat ccaccaagca atctcgtgcc ctccctcgcc
      661 tccgtgcgtg gcccaacccg tcaaggcagg cacgctcatc agaagcgcga agatgatgcc
      721 catggttttc tgtgcgtcat tgagaccgtg gctcaggctg tagaccgccg cggagaaaag
      781 ctgaagaacg cgaaacacgc ggtcgatccc ccgaggtgta atgcctttga aagcctgact
      841 gacccatgtt cccgcccaca tggtcgccac catcatcacc cagccaatga ccagaccgat
      901 cagcggcgac agaacgatga aaacggcgat cttgccgatc gtgctccaga tcagcgcatg
      961 aaagccgcct ttgacgatag cagccccgga catcgcccca atgagggcgt gggaaacgct
     1021 gatcggcatc ccatggcgag tgcagatgac cgtccagacg atcgcggcaa tcaaggtcgc
     1081 catgatgagg tggctgtcaa caacgccggc ctgcaccagc ccattgccga tggtcctggc
     1141 caccggcgtg ccaaagccga atgcggcaac aaaattgaaa aatgcggccc acaggacggc
     1201 ctttcggggc gtgagcacgc gtgtcgccac gatcgtcgca accgagttgg cggcgtcgtt
     1261 gaaaccgtta ctgaaatcaa aaaggagggc aattgcgaca ataatcagta cgtatgtgac
     1321 ttgtgggtcc atgcgtttgc cggcggctcg gaaaaaaccg catcaggtat tcttgagaat
     1381 aatcccctca atggtattgg cgacgtcctc gcaggtgtcg atggcccgct cggtaatatc
     1441 gtagatctcc ttccacttca tcgcgtagat cgggtccttg caatggttat agagatccgc
     1501 aaccgcggcg tggttggtgt cgtcgcccac gttctcaagg tgatgaatct ccaccagata
     1561 ttcatgcacc tgtcccggct tcttgaggtt cctcaacttg ggcagagcct gcccgatcag
     1621 ggcggtcgcc ttgcccagaa cctcggtctg tttgaccagc cagggggtgg gggtgtcgat
     1681 ccgatagagg accaaacgct tggcggcggc gtcgatctcg tccaccacgt catccatctc
     1741 aacgatgagc ctctggatgt cttcacgatc gaacggcgtg atgaacgtct tatcaagggt
     1801 gtcaagggtg cggtgagtca cttcgtcgcc ctcatgctca tatcccctga tgcgctggat
     1861 gatctcctct cgccgagcgt aatcgcgacc cagctcggcg taagcctggg cggcccggat
     1921 catgaccgtc gccgaggcct caaaaagctc gaaaaacttg gtatctcgcg ggataacgct
     1981 gaacaccgac gcaaacctcc cgagatccac gttccaggga atgccgcccg gcaataggac
     2041 ggcaggccgg gattgtatcc gggcattcac gggattcaaa ccaaacgcta acgagccgct
     2101 agcgcgggct caacaagccg cggcagcagg cggtcctgcg gcaggccgac gcacgcgatc
     2161 aacgattctg ccgaaggacc aagatatcaa tctaccgctg tcaaacagcg agccaccgga
     2221 ctggaataaa ctgattagct acgcattatc tccctgcttt aatggatctg ccatctcgcc
     2281 gaacgcttga ggctgttaag cagtccccga gaataccacg cgtccgcccg ggcaggtggc
     2341 gcgaggccgt tcttgttgtc gacgaggctt tcggctaagt tggcgtgctt tcctggccag
     2401 gaagtgcccg gcccgggtgc gcacaaggat gccggcgggg aacacggacg gcgaactgtc
     2461 gctgtcagga tgtacaagtc gctcctgacg gggcggcagc cacggccaca ggcaagaggt
     2521 gaggtcatgg aagccctttt gtctcacaac atgcgcgaaa aaggtgcgcc gacgctcaac
     2581 cacgcggcgg gtttcggtcg ccgcggcgac ccggacgccg ctctgccgaa cgctggcggc
     2641 gggcttcgga cgtgttctga gcatttgcgg gctcgagaat caggagggcg cagtctgcgg
     2701 ggtatgctca ttggactcct ggcagtcggc cttgcgagcg ggtgcagcaa ttggccgtcc
     2761 ggcacatcga acaagagcgg cgggctgaag caactcaatc cgcgtggtgt ggcctttctc
     2821 gaggaaatac ccgtaccgga cggttttcag ctcgtcgacg agcactcgat ggaccacgag
     2881 tcggccggac aacgaatggc gaggcacgag tacaaaggaa gagccgaccc ctttgcggtt
     2941 cgcgattttt accgccagcg gatgcctcaa atggggtggg aacgggtgtc ggaccagagt
     3001 gtcaaaggtg ttatcacgat gcgcttcgag aaaaagctcg aagcatgcac catacagatt
     3061 cgaccaggcc acttcggcca gtcgattgtc aacgttgaag ttgtgccgtt cagccgaacg
     3121 ccgatggagc cgccaaaaca gcgatgaagc agcgcagaag acaagaactg aaaacgaacg
     3181 aactgagcat ctatctcgga cagatgtatg acttcctggt tcgcaactcc acctatgtcg
     3241 tgggaggcgt cgtggtggtc gccctggtca tcatcgtggc cacaatgaac gccaggagcc
     3301 agcatcgggc caaggtggac gccatgaacc gaatcagggc cattcaaagc ggcgaggcgg
     3361 tccaggacct gaaactcctt gacgagatca aggatctcat cgccgagtat ggagatgatc
     3421 cgtggcttgg cccatcactc ctggacctcc aggcatccct ggctcaccac ttggctcttg
     3481 gtcggacatc agaggctgac aaggcggaac acgatcgcct gctcgacgag gcaagatccg
     3541 cctgtgagcg agcgattcag agattccgaa gccagcccct cgtaatggcg cgggccaagc
     3601 tggccttggc ggccgtagag gaaacgctgt tcgtcgaggg caaaggcgat caggaaagaa
     3661 ttcgaaagct gtaccaggag gtcatcgacg gccccgtcag cccctaccag agactcgctt
     3721 ccgaacaact gagcagtctg gacgccaggc tggcgaaact ggaaatcgtg gccacccgtc
     3781 cggccgccgg cgccccgaca tcagcatctg ctatcactct accgccgatc gctctgccga
     3841 cggctcccgc cactgcgacc atagccccgg cgaccgagcc cgctgccgca ccggtcggca
     3901 agccgacaga gtaaagcgcc tgacgaggct ttctgaccat gcgatggcac tgccttcttg
     3961 cagtcataac cgtcttgaca tcggttttgc ctctctgtgc cgacgaaggc atgtggttgc
     4021 tgacccgacc gccgaccgag gtgctgaagt cgaaacacgg tttcgagccg acacgagcct
     4081 ggctggagca cctccagaaa tcgtgcgtcc ggatgggagc ttcgggctcg ctggtctcgg
     4141 ccgatggatt gctcatgacc aaccatcacg tcggcgtcga cgacttgtac aagctcagca
     4201 cgccgcagcg ggatctggtg cgggacgggt tttacgctcg cacccacgac caggaactga
     4261 ggtgcccgga cctggaggtg caggtactct ggtcgatcga ggacgtcacc gagcaggtcg
     4321 cggccgccgc caccgccgag atgcccccgg gcgaggccta cgaggccagg cgcaaagcca
     4381 tgacccgcat cgagcaggaa tccaaggacg ccaccggcct ggacagcgag gtcgtcaccc
     4441 tttggtacgg ggcccgctat catctctatc gctatcgccg atacaccgac atgcgactgg
     4501 tgttcgcccc ggaaaagtcc gtcgcctttt tcggtggtga cacggacaac ttcgagtacc
     4561 cccgttttga cctggactgc tgctttttcc gcatctatga gaacgacaaa cccctcaagc
     4621 ccgagcacta tctgaaattc agtcgccggg gcgccgccga cggcgaactg ctattcgtcc
     4681 tggggcaccc ggcccggagc cgccggctgg tcaccgtcga gcacctgaga ttcctgcgcg
     4741 atgtcgagat gcccgccgtg ctcaactggc tgtggcgccg agaggtgcag ctcgccgtgt
     4801 tctgcaaccg cagcgacgag aacctgcgga tcggcgagaa tgattacttc agcgttcaga
     4861 attcgcgcaa ggcccgcacg ggcatgcaac aggccatgct ggaccctcga ttcatcctcc
     4921 aaaaggagca agctgaggcg acactccggg ccgccgcgaa gaccaccgag ccgtgggatc
     4981 gcatcgctga agcgcagcgg gtttaccgca cattcttcaa ccgacgcaac actctgcgcg
     5041 aagggcatcc cctggagtcc aagctcttcg aaatcgcgcg aacgctcgtg cgcctggccg
     5101 acgaactacc caagccctcg cccgagcgcc tccgcgaata tcgcgagtcg gaactcgagt
     5161 cggtctacct gtggctgtac tcgcctgccc cgatctacga tgaactggag atcgagggcg
     5221 tgaccagcgg tctgacgctg atggccgagc ggttcggtgc ggacgaccca ctggtggtga
     5281 aggcactcga cggtctgtca ccgcaggctc gcgcggagcg gctcgtccgc gactgcacgc
     5341 ttaaggacgt cgagacgcga aaacgttatg tcgtggaggg caagaaggcc atcgatgcgt
     5401 cggaggaccc catgatccgg tttgccgcgg ccatcgatcc tgagagccgc gccctccgca
     5461 agcgattcga agacgaggtc gaaggtgtcg agcgggaaaa ctatgccagg atcggcaccg
     5521 cccagttcgc tctctatggc gaagacttgt accccgacgc caccggcacg ttgcgattcg
     5581 ctttcggcgt cgtcaagggc tatgagcaga acggcaggca gatcccgcct ttcacagact
     5641 tcgccggctt gtacgaaagg tggaaagaac gaggcccccg gccgcccttc gacttgccac
     5701 agcgatggct cgaccgccgc gaccgtctcg acctcaagtt gccgcttaat ttcgtgctga
     5761 cagcggacat catcggaggc aacagtggca gcccgatcgt caacgccaag ggcgaggtcg
     5821 tcggccttat cttcgacggg aatctgcaat cgctggtctg ggacatcgcc ttcgacgacc
     5881 ggcagggccg ggcgatcgcc gtggacacga gagcgatcat cgaggccttg cgcaagatct
     5941 atgatgccga cgagctggcc gatgagttaa tgcgcgagtg atcccggtcc ggctgcgggt
     6001 agccgatccc cggcgcgtca aaccgcccct gctcggatcc gcgcaacgac tactcacccg
     6061 gcgatccgga tgccgcgacc tcttgccgac gtgcccgccg gatctccgaa agcatatctg
     6121 gaaggcaaga aaacacggcc ctcggccggc ccgcgccggc tgatcggcga accggcgacc
     6181 gccggcgacc gagaaccacg ggggtatggc ggcaaaccgt ttttctgcta tgattctgcc
     6241 cgaccgtttg tgaacccctg gttcaagagc acgagcatga gcaacttcac ttggattgac
     6301 ggaagcattg tcggcgtgta tctgctcgcg acaatggcgg caggcatcgc cgtccgcagg
     6361 tacgtcggca aggtggaaca ctttttggtc gccgggcgag agatgaatct ctatctcggc
     6421 atcgcttcgc tggccgcgac cgaattcggc atcgtgacgt gcatgtacac cgcccagctc
     6481 ggctacgagg cgggctttgc cggagcaacg ccgggcattc tgtacgctgc ggccatgctg
     6541 ctggtcggcc tgaccggctt ctgcatcaag ccgctgcggg acgccggcgt gatcaccatc
     6601 ccggaattat tccagaaccg ctacggctcc cgcgtccgct ggctcagcgg cgttgtcatc
     6661 gtgctgggcg gtttgctcaa tatgggcgtg ttcctgcgaa tgggcggcga cttcctggtc
     6721 caggtctgcg ggctcgaagt acgccacctt gtgccgatga tgggcatgtt gctggccgcc
     6781 gttgccatct atacgatact cggaggcatg ctctcggtgc tggttaccga cttcctacag
     6841 ttcatcgtga tgagcgcggg cctcctgatc gtcaccctgc tcacccttca caacgtcggc
     6901 tggagcaaac tggttgagac ggtcaacacg cactacggtg ccggcggctt caacccgctc
     6961 gtgcacccca agatgggcat cgactacgta gtgtacaacg gcgttgtcaa cctggccgcc
     7021 gtcctgacct ggcagaccat catccaacga ctcctggccg cgaaggacac ccgtaccggc
     7081 cgccgcatct acacccacac gagctttttc ttcgtctgtc gattcctcat cccggcgatc
     7141 tggggaatcg ccgccctctc catcatggcc ccggaccagt tggcggggat ctttcccgaa
     7201 gtcgaccagg ttcgccgatc tcttctggcc atgccgcgat atctcagcac tgccgtgcca
     7261 tggggactca tgggcctttt ggtagccgcc atgctcgccg cggacatgtc caccgactcg
     7321 gcctacatgc tgacgtgggg cggcgtgatc tacaacgata ttctggctcc cttccgccgc
     7381 aactggacgg agcggaaagg tattctcatt aatcgtgcaa tcatcgcctc catcggcgtg
     7441 tttctgttct tcttcggact ctattacgac ctcaagggag acgtgtggac ttacctgacg
     7501 gtcacgggga gtatctacct gtccagtatg tccgtcctgc tgattgcgtg ctgctattgg
     7561 aagcgggcca acagttgggg agccatggcg gccattgtcg taggggccat cgtgcccatc
     7621 acattgctgg tcctccaaca aacggaccgg tacaagccgt tttgcgacga gtggcaatat
     7681 gtgctgggcg cgatgacgta cgcgactgcg ggggcggcaa tgatcatcgg ttcgctgctg
     7741 aagccccgag gcacggttac gtcgggggcc ggggagtaaa tcgtcatgag agaacactgg
     7801 ttctggtggc tgctgaccgc ggccgtcgtc acctggtatt ccaccgtcac cgtctacgtg
     7861 gctatcaaag gcgtcaccga catccggacg atgctcagac atctcggatc ggtgaaatcg
     7921 acggagggcg aatcataagc gggagatcgg cctcgcgctt ggccgtgggc gggcgttggg
     7981 gacgcactgt tcttcgtggc aggaaccgcc tctgtttcgg ccgaggccgc tggccttgtg
     8041 ccattctcaa tctgcaatcc ccaatgcgcc gtgccccagt tccagctcgt tctcagaacg
     8101 tccggcttcc ggccgggtac cgatccagac tgaagatctc tgacaccggc ttgcggcagg
     8161 attcgtccag cgggtaattg agattcggcg acgccgggta gcccaacgtg atcatcgcca
     8221 ccaccttggc ctgcgcaggt atatccagca gcttcttgac gctggcctcg tcaaacgccc
     8281 cgatccaaca ggtacccagc ccctcgctca cggccgcgag cgtcaggtga tccagggcga
     8341 tagccacgtc cacgtcggcg ctgttgccgt agccgcccat ggccttgtag gcctgaagcg
     8401 ggaagccgca agcaacaacg aaaacagggg catcgagcat ccatttctgc ttgtgcgact
     8461 ggcgaacgat ctcctcccgc agatgggcgt ccttgacgat gatgaaatgc cacggctggt
     8521 agttgcacgc cgagggggcg aaacggatcg cctgcttcag ccgctccatc acgtcgtcgg
     8581 gaatcggctt gggcaaatag gcccgcacgc ttcttcgccg tcgaatcgca tcatgaacgt
     8641 ccatcgaatc cctccatcgg gatcagagat cccggccggg tggggcagat cgaagagtcg
     8701 ggtagggcag gtcgaagggg cgaaagccct gagacctgcc gtactacttg cggccgcgaa
     8761 cgcatccggc agccaggaca atcccaattg tagtgccgca accgataaca atcgacagaa
     8821 ccaggtgcag aggatgcatc gcggtgcccg actgtccgaa gacgggcaac aacaggtacc
     8881 acgtgccgaa agcggcgccc aatccgagca gcagagccgg cgtcacccag attgcgcgct
     8941 gcgcctccac gaatccaggc tgattccgat cgatggcgtc gagaatctgt cttcgcttgc
     9001 gccgcgatac tattgccaga cgaagatcga gcacgaaggc gaccgccaac aagctgagcc
     9061 ccacaagctg tgctccaatc atcgtgaaga cacatgctcg cagcagtctg tcggaaccgg
     9121 gtgtctgtct gaggccggca gcccgctgct caacccactg catcaagctg ggcaacatgc
     9181 caccgcagac taacaggagt gctcctgcaa gccacatgcg aaaggtccac ttggacgtta
     9241 cggggtgcga tcgtttaccg gcttccccga cgtactgctc caccagcagc ttgccgtgat
     9301 cgaattcacg cccgcactcg ggacatcggc cgtctggaag accacgcaga aggtattcgc
     9361 actttggaca ggaaacggga aaccgctcct ctgacaaaag cgagggatca acacgcgtat
     9421 cgcgcgggat gaccagtctg cgcgcagccg cccatcggag gaatcgctgg tagcgcgatg
     9481 ggggtgtctt gtccatatcg cagccgggtg gggcaggtcg aaggggcgaa agccctgaga
     9541 cctgccgatt cttcttgctg aaagccgacc gctgaccgct tcccctaata gcggcaggtc
     9601 ttgcggcctt cggccgccac gaccgtgccc tacgacagcg cccgcaccac cgactgcggc
     9661 cgcagataat cgaacagggt ctcgggtccc agcacgcccc cgccacaacc ggagaggttg
     9721 cagcctgcat agggcacgcc gtgcggaaac agattgtgac cattgatcca actgttgccc
     9781 gcgaccaagg cctcggccac gcgattggcc cgcttcaggt cagcggacca gacgctgttg
     9841 gccagaccat aattggaccg attgaccagc tcgacggcct cctcctcttc gctgaacttc
     9901 atcaggaacg aaacggggcc gaagatctct tcgcgagcac agacgttatc tggatcaccg
     9961 gtgagcatgc atggcttgac atagaagccc tcagggtgct cggtaaccgt cgctggtccg
    10021 ccagagagaa cgaactccgc tccttgggcc ttgccctttt cgaggtatcc gagaatccgt
    10081 tgccgctgct tctcgctgac cgccgggccc atctgggttg acggagccag gccgtggccg
    10141 atcttgatct gctgcatggc ctcgcgagcg atcttcacga acgggttcca gatcttctcg
    10201 tggatcagcc accgtgtggc ggtgcagcag acctggccgg tgttgagggt cacggccgcg
    10261 accagtttct gagcgacaac agccggatcc acatcgtcaa acaccacggc agcccccttc
    10321 ccacccagtt caagcttgac gggaacgagg ttgttgccgc aagcggaggc gatcaggcgg
    10381 cccacctcgg gcgagcccgt gaagcccatg cggttgatac ccgggtgctc ggccagggct
    10441 ttgccggccg tgtggccgta gcccggaacc acgttcacga cccccgcggg gatacccgcc
    10501 tgctcgacga gcttgcagaa gtacaacgtg ctcagcggtg tgtcctcggc cggcttgatc
    10561 accgctgtgt tgcccgccgc cagcgctggc gaaaggttcc accccaccag cagcagcgga
    10621 aagttccacg gcaagataaa cgccgccaca ccgtagggaa accgcacttg tcgggcctca
    10681 aagccggaga cggcaatcag ctcgcttcgc cgcgtgcgaa ccatcagatc ggcgtaccag
    10741 cgaagcgtct gagcaacgtt aggcacgtcg aaacccgccg cctgcgacag gggcttgccg
    10801 acatcaagcg attcaagttg ggcgagcgtt gcccgattct ggtctaccag gtcggccagt
    10861 cgatgcagaa acacggcccg ctcgttggcc ggcatcgtgg cccaaccgct cttgcggaac
    10921 gcctcgcgag ccgcctggac agccttgtcg acgtcggccg ccttgccctc ggccacagtt
    10981 gccaagggtg agccgtcgcc gggatcgatt gtctgcagga cgccgccgtc ggctgcctca
    11041 atccaccgcc cgccgacgaa catcggaatc ggatcctgca acagaaaggc ctgcacctca
    11101 gggagcaatc cgctgatcgt tgccatggct tcacctcgcc ttaaagggtg atcaaccctg
    11161 ttcctggacc ggccccagcc actgctggcc aatcacggcc agccaccgct ctccgtaccg
    11221 gccaacctat cccggcgagc caagtcgggc aagagcccga accggtcaac ccctgaccac
    11281 ggcaaaggtg cttcacacat gctattatgc agcaccggac cattggggcc tgttggaggc
    11341 acgtgagtat gggttcgcct acacaccgag ttgaccatcc gtacgcctcc cgacgtccag
    11401 atatggcggg cggttcgccc gtggtcgctg gaacgaaatt cccggtccgg tccctggtcc
    11461 agtacgtact gaaacagggt atgtcaccag aggaactggt tagtcccttc cgccgcgact
    11521 ggtgcgcatt ttgcgcgcat gtttctggat cgcag
//
LOCUS       DAOTPW010000180        11394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5374_length_11394_cov_5.739130, whole genome shotgun sequence.
ACCESSION   DAOTPW010000180 DAOTPW010000000
VERSION     DAOTPW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5374_length_11394_cov_5.739130"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(568..1473)
                     /locus_tag="P5316_15365"
     CDS             complement(568..1473)
                     /locus_tag="P5316_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86386.1"
                     /translation="MTFRRIRARHPGMSGYARNVLPAARAVRPKSTPDDSGAFCFADA
                     RYQYRSGSIPSGRSRSCVAVEQLTAEAMRPCGYCRDGHERCGVCQGRGCSEDRDTCDH
                     CLGLGSCTCGFCGGSGLVTCDAVAPELRVAVAIERIKEASRQLDAITRQPSTPSAGSG
                     SQQSLDEVGRTLLALNRVAAMIEDSLHLLQAETQNRPGGEQDLSRVIRFGLKRAAECI
                     LQMRAIINGMAVSARQLARQSKRRTKEREIAVKRAAFYASLLRRPELLTGTGFERVLL
                     IAAIEKLRKASASPTAGQGRPQEEP"
     gene            complement(1544..1894)
                     /locus_tag="P5316_15370"
     CDS             complement(1544..1894)
                     /locus_tag="P5316_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRR86387.1"
                     /translation="MRVQIFARNVTLTPSWVDFIERRLTFALGRFASRVQDVVVRIQD
                     LNGPRGGIDQQCQIDARILPSAWKTAKGTGADLGSAISIAVDRIARCVRTDLDRRRTR
                     RIRGRKRMETPVLA"
     gene            2029..2931
                     /gene="nhaR"
                     /locus_tag="P5316_15375"
     CDS             2029..2931
                     /gene="nhaR"
                     /locus_tag="P5316_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HRR86388.1"
                     /translation="MESLNFNHLRYFWMVAREGTIAAACDQLYLTQSTISTQIRALED
                     YLGAKLFDRVGRNLVLTETGQTVYRYATEIFALGNELVDAARGCPAGPPARLSIGAQD
                     TLSKLVVRRLLEPVFRLPEPLKLMCYEGTPLQLLPKLSTHEVDLVFSDAPMPPQIKVR
                     AFSHLLGECGIAFFAVSDLARQLREGFPESLSGSPALLPAETTAMRGTLERWFRTMRV
                     HPTVVAESEDVELIMALGQQGRGFFPAHNVIAPEVARNWQVEMIGEIGGHTERFYAIS
                     VERRLNHPAVVAITEAARKMLSVH"
     gene            3611..5584
                     /gene="speA"
                     /locus_tag="P5316_15380"
     CDS             3611..5584
                     /gene="speA"
                     /locus_tag="P5316_15380"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HRR86389.1"
                     /translation="MGKLYHPVFTEKTDAVRKWTVSDAMDLYNIRNWGHPYFSINDNG
                     NACVHPQGPHGPMLDLKQLVDELQRRGISLPILIRFSDILRARVEALNEAFHRAIQEY
                     GYKNRYLGVYPIKVNQQRQVVEEIVTYGTPYQFGLEAGSKPELLAILALLDSTESLII
                     CNGYKDLEYIEMALLASKMGKAVTIVVEKFTELPLIMEASQRLNVVPRLGVRVKLSSR
                     GSGKWEFSGGDRSKFGLTIAELVQAVDYLRGNGMLDCLQLLHFHLGSQITNIRSIREG
                     LEEACRIYVEMVQMGAPLRFLDVGGGLAVDYDGSKTNFASSRNYSMQEYANDVVSAVL
                     DACDGAEVPHPVIVSESGRAITAHHSVLIFNVLGVSSYSETEVPQEIPEDKPDVLRNL
                     LDCCRNITRKNFQESYHDALHYREECMSLFRHGYLTLKDRSMAESIFWAAMQRILRIV
                     QELEYVPDDLEGLRRSLADTYFCNFSCFQSLPDFWAVQQLFPILPIHRLNEKPTRQGI
                     LADITCDSDGKIDRFIDLRDVKDALELHPLNGEDYILGVFLTGAYQETLGDMHNLFGD
                     TNVVHIRLGPEGGYVIEQLVKGDTIKEVLQFMQYSTTELSNRMRKTVEAAVRSGTISF
                     EEAGHFLEKYERGLEGYTYLERRVPRPTVADHV"
     gene            5577..6350
                     /locus_tag="P5316_15385"
     CDS             5577..6350
                     /locus_tag="P5316_15385"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRR86390.1"
                     /translation="MSDSSLPPWAHWLKRCLPAVALVAASLLLLRFDVRILRWGNRGF
                     PDDPHGWTEQFLLGLRDFGQILPVIVTCVVVAAFDRRWRRVVAAILLSQLVAMVVYNG
                     GKYTIVRYRPNSGVVDFDDPATRPADTWVGWAPGNTDDRSSSFPSGHSAAAFAFGTVL
                     ATFYPRLAWLFWTLAFGCAASRFIQFFHWPSDCLVGGTIGYLSACLVLGIFGPGHKRP
                     LGKRTEPVPGTAQGLSSDPAEQVVMTSAGGPRNDRLCCR"
     gene            6706..7848
                     /gene="tgt"
                     /locus_tag="P5316_15390"
     CDS             6706..7848
                     /gene="tgt"
                     /locus_tag="P5316_15390"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRR86391.1"
                     /translation="MFELEIHHCEPGGQARVGVLHTPHGPVRTPAFMPIGTRGSVKGL
                     WPEQVRDSGADIILANTYHLALRPGAELIEQLGGLHRFIGWDGPILTDSGGYQVFSLA
                     ELNHITDDGVTFRSHIDGQWLHLDPVSATRIQNQLGADIIMAFDECPPGNAGREAVAQ
                     AVDRTVRWAAGCKQAHERDDQALFGIVQGGVFHDLRRLCADRLIDLDFDGYAIGGLSV
                     GETHRNMVEVLNEVAPHLPPNKPRYLMGVGTPRDIVAAVRAGVDMFDCVLPTRNGRNG
                     YAFTATHPLRLRNEKHRAENLPLEAECPCPTCRRFSRAYIRHLFLAGEMMGPILLSLH
                     NLWFFQRFMARLRDLIPSGDWETLLAEFPVAADGGLSRIDDKGACE"
     gene            7845..8180
                     /gene="yajC"
                     /locus_tag="P5316_15395"
     CDS             7845..8180
                     /gene="yajC"
                     /locus_tag="P5316_15395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRR86392.1"
                     /translation="MTHLFDIPVVAQGGGGPPTMWIGLILMIVVFYGLVAWNSRKDKK
                     RRQDMLNAIKKNDRVMTIGGIIGTVVSTNDTEVTLKVDESANVKITFARGAIQKVLTE
                     GERPADVGS"
     gene            complement(8255..10219)
                     /locus_tag="P5316_15400"
     CDS             complement(8255..10219)
                     /locus_tag="P5316_15400"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRR86393.1"
                     /translation="MTGHPAAENRTARELHLATQEYLPLFPKRTRWLWWPSALAGVVG
                     LALTYGFDEPAVDDRRIHQILLAAAILFLLSRIMLLIPPVEISARIGRCWLDFAVLLA
                     GAVWWGLDHDRQREVIEIIAGYSAAIGFLAASQAGVKWLIEGPLPGVHPGPVRRLFLA
                     GLFLAIVGGLILSLPRCWARGYPASSESGYTGQFRYEVGAHALNCLFTSASAITGTGL
                     SVHDIGHDFSLGGQIVILLLMQIGGLAILAIGTVLGLRLRAMLGWETHQDDTTPEGLR
                     RAVRFSCLLLLTIEVIGLLAMFFAQPRLFDSSEPLRPKIVASVFHTISAACNVGLTLN
                     SDSMVGARGLATTYAVILPLMILGGIGGPFFFSIYRKLRDRTARMPVDVWLTLVATVL
                     LVVIGAGLLYGIESTYTWQLRYPQEKTLGSLQWNGASSAPAGEIVFGSGSSERVQAER
                     MSTMRRGPRIAAAFFQSVTARTCGMRTARLDDKSLSPASRYLLMVWMLIGGSVGGTAG
                     GVRICVVGLLLLAIVRPRGRLWRSEASLSHGTGRQTLAAAGVVVAAMALVIVVTSFVL
                     IYRQRDASSGSYVYESSSALFESISACTNTGLSAGLTAQLPTEDHLSGPVWLRVTSRS
                     VLILAMLLGRVLPVGILLRWAAIKGRQPPP"
     gene            complement(10216..10617)
                     /gene="hisI"
                     /locus_tag="P5316_15405"
     CDS             complement(10216..10617)
                     /gene="hisI"
                     /locus_tag="P5316_15405"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRR86394.1"
                     /translation="MEHYMQGLDNLKYDANGLIPAIIQDADTGEVLMMAYMNKHSLEE
                     SIRTGKTHFWSRSRRRYWMKGETSGHTQEIQEIFIDCDADTILVKVKQNGAACHEGYR
                     NCFFRKYDAVTEQWSVCARRLVNPTKVYGNK"
     gene            complement(10759..>11394)
                     /locus_tag="P5316_15410"
     CDS             complement(10759..>11394)
                     /locus_tag="P5316_15410"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRR86395.1"
                     /translation="PLVEESDELDLKAATAEVDRVARDLLPGSRVGLLHGRMKNAEKQ
                     TVMREFAAGRLDALVCTTVIEVGVDVPNATVMVVQHADRYGLAALHQLRGRVGRGSKA
                     SICLLMTDSPSQLANERLEILCRTTDGFRIAEEDLRLRGPGELLGTRQHGWPEFRVAN
                     LVEDTEILMQARDDAARLVREDPNLKNPLHARLKAELRRRFRDKVAFISVG"
BASE COUNT         2173 a         3518 c         3450 g         2253 t
ORIGIN      
        1 tcgccaaggg agttttcgcg cttgacaaga cgggggctaa tgggtgaatg ctgaccgctc
       61 ttgtcaaccg gtgagtacgc cgagaacgcg gaacaacatg acctcctgtt accaccttta
      121 ctgaaaatgg cttctgagcc cttttccatc cgcccctttg ccatccgaga gagccgtgcc
      181 catggtgacg cggcgactat tctgcgcggg cccgcggcaa gactccgcgc cctgccaagc
      241 gaggcgggcg gtcgcgacgt gcatcgccgc tacgccggga ggtcagcacc ggattaagga
      301 atccagaagg gaggtgacac atcaacaaca ccggctccgg cccgtgcggc tatccgagga
      361 acacttcagg tggcacccgt gggggtgtaa ggggcctgcg cggcagggcg caaacacgct
      421 ccttgacaga actgaagccc tggtgaggtt gcgggttcat cccagaatca acgtctgacc
      481 gcttccggtc gcgagttttc ccgcagggct tcatctcgca gcgcccatcc gcggcaaccg
      541 cccacgaatc ttcgggtaaa cgagttgtca tggctcctcc tgcggccggc cctgcccggc
      601 ggtcggcgag gccgatgcct tacgcagctt ctcgatggct gcgatcaaca gcaccctttc
      661 aaaaccggta cctgtcagca gctcgggccg tctcagcaac gacgcgtaga aagcggcacg
      721 cttgacggcg atttcccgct ccttcgtgcg acgcttgctc tgtctggcaa gctgccgagc
      781 cgaaacggcc atgccattga taattgcgcg catctgcagg atacactcgg cggctcgttt
      841 caagccgaac cggatgaccc gcgacagatc ctgttcgccg cccggtcggt tctgggtctc
      901 tgcctggagc agatgcagcg agtcctcgat cattgccgcc acccggttca acgccagcag
      961 ggtccggccg acctcatcga gactttgctg agatccgcta cccgcggagg gggttgacgg
     1021 ctgacgcgtg atggcatcga gctgccgcga agcttccttg attcgctcga tagccacggc
     1081 cacccgcagt tcgggggcaa cggcatcgca ggtgaccagg cccgatcctc cgcagaaacc
     1141 acacgtgcag ctgccaagcc ccaggcaatg atcgcaggtg tcccggtctt cagagcaacc
     1201 tcgcccttgg cacacgccgc atcgctcgtg cccgtccctg cagtagccgc aaggacgcat
     1261 cgcctcggca gtcaactgct ccaccgccac acagcttcga gacctgccgg aaggaatgct
     1321 gcccgaacga tactgatagc gtgcgtccgc gaaacagaac gctccggaat cgtcgggtgt
     1381 ggattttggg cgaaccgccc gagccgccgg cagcacgttt ctcgcgtacc ccgacatacc
     1441 ggggtgtcga gctctgattc ggcggaatgt catctcacac ctcgcttccg gtcattcagc
     1501 cccgagctcc tcaggcccgg gacacctgcc cgttacccgg tactcacgcg aggaccgggg
     1561 tttccatgcg cttacgcccg cggattcgac gggtccgtcg gcgatccagg tccgtccgga
     1621 cgcaccgagc gatccggtcc acggcgatgc tgatcgccga acccaagtcg gcacctgtcc
     1681 ccttggcagt cttccacgca gagggaagaa tgcgtgcatc gatttggcac tgctgatcga
     1741 tcccgccgcg cggaccattg aggtcttgaa tccgcacgac cacatcctga actcgtgagg
     1801 cgaatcgccc gagggcgaac gtcaaacgtc gctcgataaa gtccacccaa gagggggtca
     1861 gagtgacgtt cctcgcgaat atctgaacac gcatgtagac ctcccttctt tagctgatgg
     1921 tgccgtatcc accggcactg acgtcgtatt ctacgcccgg ctaatgtcat tagtttcatt
     1981 caaaatatgc aattgaccat tcgattatat cgatatataa tgatgaccat ggaatcactt
     2041 aacttcaatc atttaaggta cttctggatg gttgcccggg aaggtacgat cgccgctgcg
     2101 tgcgatcaat tgtatctgac gcagtccacg atctccaccc agattcgagc cctggaggac
     2161 tacctggggg ccaagctctt tgatcgcgtt gggcgcaatc tggttctaac ggagaccggc
     2221 cagaccgtgt accgatacgc cacagagatc tttgccttgg gcaacgaact ggtcgatgcg
     2281 gcgcggggtt gtcccgcagg tccgcccgct cgcctgagca tcggagctca ggacacgctt
     2341 tccaaactcg tggtccgtcg cctgctcgaa ccggttttcc gtctgcctga gcccctaaag
     2401 ttgatgtgtt acgagggaac tcctttgcaa ttgctgccca agctgtcaac gcacgaggtt
     2461 gatttggtct tctcggacgc ccccatgccg ccccagatca aggtccgagc cttcagtcat
     2521 cttctcggcg agtgtggcat tgcctttttt gccgtctccg atcttgcccg acagttgcgt
     2581 gagggctttc ccgagtcgct gtccggatcg ccagccctgc tcccggccga gaccacggcc
     2641 atgcgcggta cgctggaacg gtggtttcgt acgatgcggg tccaccccac cgtcgtggcc
     2701 gaatccgagg atgtcgaatt gatcatggcc ctggggcagc agggacgggg cttctttccc
     2761 gctcacaatg tgatcgctcc ggaggtcgcg cggaattggc aggtcgagat gatcggcgaa
     2821 atcggcggtc acacagaacg gttttacgcc attagtgtcg agcgccgact caatcacccg
     2881 gccgttgtgg cgattaccga ggcggcacgc aagatgcttt ctgtgcactg agagcatcgc
     2941 ggagggaggc gagcctgtac ctgtgacttg ccacacacgt gctgcctggg cggcgggtcc
     3001 caacacacca gaggtctcgc ctggcggtcg ccccgcgtgg tatccggttt atcccggcgc
     3061 gtcggcttgg tcggtgtcgg cggcgcccgt tgctccaggc aggtttcaga aggaccgttc
     3121 ttcgaggcag ggcatgcggc cgaaaccgtc tggcacaaaa ctccactgcc gcagacgaaa
     3181 caggtggggg tgtccggtcg gtctggcggg cgctgccgcg cggaggttcg ggtcgctgcc
     3241 ggaggagtcg gacccggtgg ccaagccgcc gttgcccagc aagccatctg ccggaattcg
     3301 ccgggtggct ccgactcaag ctctggcatc tcacggtcga tgaaaaatct tgcaagaatt
     3361 gtgcccaaca tcttgacctt cggaaaaccg atgccacaat agatcgggat cgcgggacag
     3421 gcgggcgttt ggcgcgtgcg atctctcgcg agcgtctgct cttgagcgag cgatcaaaga
     3481 ggcaggtgtc ctgggcttcg aacgacaagg ctccgtgtct gaggttcctt gcgagacgat
     3541 gaggaatcgc cattgagttg attgggggac cgctgcatgg agggggcggt ggtcaacgag
     3601 gactgcttga atgggcaaac tctatcatcc ggtcttcacc gagaagactg atgccgttcg
     3661 caaatggacc gtcagtgacg cgatggactt gtacaacatc cggaactggg gtcatcctta
     3721 cttctcgatc aatgataatg gcaatgcctg tgtacacccc cagggtcccc acggcccgat
     3781 gctcgatctc aagcagttgg tcgatgaact ccaacgccgc ggcatctctt tgccgatcct
     3841 gatccgcttt tcggacatcc tgcgggcacg cgtcgaagcc ctgaacgagg cgttccaccg
     3901 agcgattcag gaatacggct acaagaaccg ctacctgggt gtatacccga tcaaagtgaa
     3961 ccagcagcgg caggttgttg aggagatcgt aacctacggt acgccttatc agtttggtct
     4021 tgaggccggc tccaagccgg agcttctagc cattctggcc cttttggatt ccaccgaatc
     4081 gctcattatc tgcaacggct acaaggactt ggaatacatc gagatggctt tgctcgcaag
     4141 caaaatgggc aaggcggtca ccatcgtcgt cgagaaattc acagagttgc cgctgatcat
     4201 ggaagcctcc cagaggctga acgtggtgcc ccggctgggc gtgcgggtga agctgtcaag
     4261 ccgcggctcc ggcaagtggg agttctcggg cggagatcgt tccaagtttg gtctgactat
     4321 tgctgagctg gtccaggcgg tggattatct tcggggcaac gggatgctgg attgtcttca
     4381 actgctccac ttccacctgg gaagccagat caccaacatc cgctcgattc gcgaggggct
     4441 cgaagaggcc tgccggatct atgtcgagat ggtccagatg ggggccccgc ttcggttttt
     4501 ggacgtcggg ggcggcttgg cggtcgacta tgacggctcc aagaccaact ttgccagcag
     4561 ccgcaactac tccatgcagg agtacgcgaa tgacgtcgtc agcgcggttc tggatgcctg
     4621 tgacggagca gaggtgcccc acccggtcat cgtctccgag tccggccggg cgatcacggc
     4681 gcatcactcg gtcctcatct tcaacgtcct gggggtcagc tcatacagcg agaccgaggt
     4741 gcctcaggag atccccgaag acaagcccga cgtgctccgc aacctgctgg actgctgccg
     4801 gaatatcact cgcaagaact tccaggaaag ctaccatgac gccctgcatt accgcgagga
     4861 atgcatgagc ctcttccggc atggctacct gaccctcaaa gacaggagca tggccgagtc
     4921 gattttctgg gcagccatgc aacggatcct gaggatcgtg caggaactgg agtacgttcc
     4981 ggacgatctg gaggggcttc ggcggtcgct ggccgacacc tacttctgta atttctcgtg
     5041 ttttcagtcc ttgcccgact tctgggccgt ccagcagctt tttccgattc tgccgattca
     5101 ccggcttaac gaaaagccca cgaggcaggg gattctggcc gacatcacct gcgactccga
     5161 cggcaagatc gaccgattca tcgacctgcg tgatgtaaag gatgctttgg agttgcaccc
     5221 ccttaacggc gaggactata tcctgggcgt gttcctgacc ggggcttacc aggagacgct
     5281 cggcgatatg cacaacctgt tcggcgacac gaacgtcgtg cacatccgtc tcggccccga
     5341 gggcggctac gtgatcgagc aactggtcaa gggggatacg atcaaggaag tccttcagtt
     5401 catgcagtac tccacaactg agctgtcgaa ccggatgcga aagaccgttg aggccgccgt
     5461 ccggtcgggc acaatctcct tcgaggaagc cggtcacttc ctggagaagt acgagcgggg
     5521 gctcgagggc tacacgtatc tggaacgtcg cgttcccagg ccgaccgttg cggatcatgt
     5581 ctgattcctc tctacccccg tgggcacatt ggctgaagcg ctgcctccct gcggttgccc
     5641 tcgtcgcggc gtcgctgctc ctgttgcgtt tcgacgttcg gatcttgcga tggggcaata
     5701 ggggttttcc tgatgatccc cacggctgga cagagcagtt tttgttgggc ctgcgggatt
     5761 tcgggcagat tctgccggtg atcgtgacct gtgtggtggt agcggcgttc gaccgccgtt
     5821 ggcgtcgggt ggttgcggcc atcctgttgt cccaactggt ggcaatggtc gtttacaacg
     5881 gcggcaaata caccatcgtg cgataccgcc cgaactccgg cgtggttgac ttcgatgatc
     5941 cggctacccg gccggcggac acctgggtcg gctgggctcc cggcaacacc gatgatcgca
     6001 gctcgtcatt tccctcgggt cactccgcgg ccgcatttgc ctttggcacg gtgctggcaa
     6061 cgttttaccc gcgcctggcc tggcttttct ggacactggc cttcggctgc gccgcttccc
     6121 gattcatcca gttcttccac tggcccagcg attgcctggt gggcgggacc atcgggtacc
     6181 tgagtgcctg tctggtgttg gggatctttg gacccggcca caaacgcccc cttggcaaac
     6241 gaacagagcc ggtcccgggc accgctcagg gcttatcgtc ggatcccgcc gagcaggtgg
     6301 ttatgacctc agcgggcggg ccaaggaatg accgtttgtg ttgccgatag cctgttcccg
     6361 gctctgctcg ttcgcctgga cgcccgccac gatcaggccg tcacaactcc tgaagaccgt
     6421 gcggaccgag cgcatcctgt tcgtggagat ccagccatag gacgtcaccg aggccctgac
     6481 cggcgaccgt tacttcaaac aggcgtcgtt ggcgacactc ttgaatcagc gcatgccact
     6541 cacccccgag tctggccact cttggttgga gtgctttgag tcatcgcctg cttatgtcga
     6601 gggtttgccg gcggggctct tccgggatgc ttgccggcaa cccagatcgg cagggtcttg
     6661 gcccagcggt ccatgttccc ggccgatgag gttatcatat ccccagtgtt tgagctggag
     6721 atccatcatt gtgagccggg cgggcaggcc cgggtcggcg tcttgcatac gccgcacgga
     6781 ccggtacgta cgccggcatt catgccgatc ggcacacggg gctcagtcaa gggcctgtgg
     6841 cccgagcaag tccgcgacag cggcgcggac atcatcctgg ccaacaccta tcaccttgcc
     6901 ctgcggccgg gagcagaact tatcgagcag ctcggcgggc tgcatcgctt cataggttgg
     6961 gacggcccga tcctgacgga cagcggcggc taccaggtct tctcgctggc ggagttgaac
     7021 cacattaccg atgatggtgt gactttccgc tcgcacattg acggtcagtg gcttcatctt
     7081 gaccctgtct cggccacccg gatccagaat cagcttggcg ccgacatcat catggccttt
     7141 gatgagtgtc ctcccggcaa tgccggccgc gaggcggtgg cccaagctgt ggatcgaacc
     7201 gtccgctggg ccgccggctg caagcaggct cacgagcggg acgatcaggc cttgttcggt
     7261 atcgtccagg gcggggtgtt tcacgacctg aggcgattgt gcgccgatcg gcttatcgat
     7321 ctggactttg acggatacgc gatcggcgga ttgtctgtcg gcgagacgca ccggaatatg
     7381 gttgaggtac tgaacgaagt ggccccccat ctgccgccca acaagccgcg gtatctaatg
     7441 ggcgttggca cgccgaggga cattgttgcg gccgtccggg cgggggtgga catgtttgac
     7501 tgcgtcttgc caacgcggaa cggccgaaac ggttacgcat tcacagcgac gcatccgctc
     7561 aggcttcgca atgagaaaca ccgggccgag aacctgcccc tggaggccga atgcccctgc
     7621 ccgacgtgtc ggcggttcag cagagcttac atccggcacc tgtttctggc cggcgagatg
     7681 atgggcccca tccttctgtc tctgcacaac ttgtggttct tccaacgatt catggctcgc
     7741 ctgcgagact tgattcccag tggggattgg gagacactgc ttgccgagtt cccggtagcc
     7801 gctgacggcg gcctcagtag aatcgacgac aaaggagcct gcgagtgacg cacttgtttg
     7861 acatacccgt tgttgcccaa ggtggcggcg gcccccccac catgtggatc ggccttatcc
     7921 tgatgattgt ggtgttctat ggtttggttg cctggaacag ccgcaaggac aagaaacgtc
     7981 gtcaggatat gctgaacgcg atcaagaaaa acgatcgcgt catgaccatc ggcggaatca
     8041 ttggcacggt cgtgagcacc aacgatactg aagtgactct caaagtcgac gagtcggcca
     8101 atgttaagat cacctttgct cggggtgcca ttcagaaggt tttgaccgaa ggcgagagac
     8161 ccgccgacgt gggcagttga cccccaagag aaaagctttt tgggccgcag agaagtcaag
     8221 acagaccact gacgcggctc tgcctggatg ccgttcatgg agggggctgt ctgcccttga
     8281 tagcagccca ccgcagaaga atcccgaccg gcagcacacg accgagaagc atggccagaa
     8341 tcaacacgct gcggctggtc acgcgcaacc acaccgggcc gctcaggtgg tcctcggtcg
     8401 gtagttgggc ggtcaacccc gcggacaggc cggtgttggt gcaagcgctg atcgactcaa
     8461 atagggcgct gctcgattca tacacatagc ttcccgatga ggcgtctctc tggcgataga
     8521 taagcacgaa actcgtgacg acgatcacca acgccatggc tgcgacaacg accccggccg
     8581 cagccagcgt ctgtcgcccg gtgccgtggc tcaagcttgc ctcacttcgc cacagccgtc
     8641 ctcgaggtcg aacaatcgcc agcaagagca gcccgacgac gcagatccgc acgccgccgg
     8701 ccgttccgcc caccgagccg ccgatgagca tccagaccat gagcaggtag cggctggccg
     8761 gagagagcga cttgtcatcc aatcgcgcgg ttcgcatgcc gcatgttctg gccgtaacgc
     8821 tctggaagaa cgccgccgca atccgcggac cgcgccgcat cgtcgacatt cgctcggcct
     8881 gcacccgttc gctgctgccg gagccgaaaa caatctcacc agcgggagcg gaggaggcgc
     8941 cgttccactg caggctaccg agcgtcttct cctgcgggta tcgaagctgc catgtgtatg
     9001 tgctctcgat cccgtacaac aggcccgcgc cgatcaccac cagcagaacc gtggcaacca
     9061 gtgtcagcca aacatccaca ggcatgcggg cggtacgatc gcgaagcttg cgatatatgg
     9121 agaagaaaaa gggcccgccg atgccgccaa gaatcatcag cggcaggatc acggcgtagg
     9181 tggtagccag cccgcgggct cctaccatgc tatcgctgtt gagtgtcagc cccacattgc
     9241 aggccgcgct gatcgtatgg aacaccgaag ccacgatctt cggccgcagg ggctcggaag
     9301 aatcgaagag tctcggctgc gcgaagaaca tcgccagaag accgatgact tcgatcgtta
     9361 gaagaagaag gcacgagaac ctgactgccc gccggagtcc ctcgggcgtg gtgtcgtcct
     9421 gatgagtctc ccagccgagc atggcccgaa gtcgcaagcc gagcactgtg ccgattgcca
     9481 atatggccag tcctccgatc tgcatcagca ggagaatgac gatctgcccg ccgaggctga
     9541 agtcgtggcc tatgtcgtgg accgacaggc cggtgccggt gattgccgac gcggaggtga
     9601 acaggcaatt aagggcgtgg gctccgactt cgtaacggaa ctggccggtg tagccgcttt
     9661 cggacgaggc gggatagcct cgagcccagc atcgcggcaa cgacagaatc aacccgccga
     9721 cgatcgccaa gaagaggccg gcgagaaaca gccgccgaac cgggccggga tgcacgccgg
     9781 gcaggggccc ttcgatcaac cacttgacgc cagcctgaga cgcggcgagg aatccgatcg
     9841 ccgcgctgta gccggcgata atctcgatga cttctcgctg gcggtcgtga tccagccccc
     9901 accacaccgc cccggccaga agaaccgcga agtccagcca gcaacgcccg atccgagcgg
     9961 agatctcgac aggggggatt aacaacatga tccggctgag caggaacaag atggccgcgg
    10021 cgagcaggat ctggtggata cgccgatcgt caacggccgg ttcgtcgaag ccataggtca
    10081 gagcaaggcc caccacccca gcgagggcgg acggccacca taaccatcgt gtacgtttgg
    10141 ggaacagcgg caggtattcc tgggttgcca agtgcagctc tctcgcggtc cggttttcag
    10201 cggcgggatg accggtcatt tgttcccgta cacctttgtc ggattcacca gacgcctggc
    10261 acaaaccgac cactgttcgg ttaccgcatc atatttgcga aaaaagcagt ttctgtaacc
    10321 ttcatggcag gccgcgccgt tctgcttgac cttcacgagg atcgtgtcgg catcgcaatc
    10381 aatgaagatc tcttgaatct cctgggtgtg gccgctggtt tcacctttca tccaataccg
    10441 ccggcgcgat cgcgaccaga agtgcgtctt gcccgtccgg atcgactcct ccaggctgtg
    10501 cttgttcatg taggccatca tcagtacttc gccggtgtcg gcatcctgga tgatcgccgg
    10561 tatcaggccg ttcgcgtcgt acttgaggtt gtcaagcccc tgcatatagt gctccatcgg
    10621 gaaagcgctg gtttcgggtt tgcagccgtc agcggctctc catgtcggtc gttggtcggg
    10681 ccggaacatc cgccgccgcg gctgatcgcg aaacgcacca gccttggcca gtctcctcga
    10741 gccggacccg atactctatc atccgacact aatgaaggcc actttgtcgc ggaagcgtcg
    10801 gcgcagctcg gccttcagcc gtgcgtggag agggttcttc aggttcggat cttcgcggac
    10861 gagccgggcg gcatcgtcgc gtgcctgcat caggatttcg gtgtcctcca caaggttggc
    10921 cacccggaac tcaggccatc cgtgctggcg ggtgcccagc agttcacccg gaccgcgcaa
    10981 acgcaagtct tcctcggcga tgcgaaaacc gtccgtggtt cggcagagaa tctccagtcg
    11041 ctcgttggcc agttgggagg gcgaatcggt catcagaaga cagatcgagg cttttgatcc
    11101 tcgaccgacg cgcccgcgaa gttggtgcaa ggccgccaag ccatagcgat cggcgtgctg
    11161 aacgaccatg accgtggcgt tcggcacgtc gaccccgacc tcgataaccg ttgtacacac
    11221 gagggcatca agacggccgg cggcgaattc ccgcatcacc gtctgcttct cggcgttttt
    11281 catgcgtccg tggagcaagc ccaccctgct tcccggcagc agatcccggg ccacccggtc
    11341 gacctccgcg gtggctgcct tgagatccag ttcgtcggac tcctcaacga gcgg
//
LOCUS       DAOTPW010000181        11370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5385_length_11370_cov_5.836500, whole genome shotgun sequence.
ACCESSION   DAOTPW010000181 DAOTPW010000000
VERSION     DAOTPW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5385_length_11370_cov_5.836500"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="P5316_15415"
     CDS             <1..538
                     /locus_tag="P5316_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR86396.1"
                     /translation="GRLDLLVNNAGVAPKQRVDILQAGEETFDRLISINLKGPYFLTQ
                     LAANRMIAWKKAGRITTPRIVFITSISAYTSSPARGEYCVSKAGLSMAVRLYADRLGE
                     HGIPVIEIRPGIIQTPMTSVVKDKYDKLIAEGLLINRRWGLPEDVAAVVAAVARGDLD
                     YSTGESIEVSGGFNVRRL"
     gene            547..2619
                     /locus_tag="P5316_15420"
     CDS             547..2619
                     /locus_tag="P5316_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRR86397.1"
                     /translation="MIPRSSADLNGLSIPVYSLNTVIVGSGAAGLNCACRLFRELEEM
                     GIENPADHLALVTRGIGLGTSNNSGSDKQTYYKMGTGGEPDRPTDFADTLTAGGCTHA
                     DVAFIEGANSLRAFYRLVDLGVPFPHTADGVFVGYKTDHDPRQRATSAGPWTSRFMVR
                     KLLAELERYGIPVFNRHHMLAVVTGREEGRQAACGILCVDLARQNDDNHGLVLFNARN
                     VVAAGGGPGDLYEISVYPPGQMGPYAALFEAGAEAHNLTESQFGLASLRPRWNLSGTY
                     QQVIPRYYSTDADGEDEQEFLNPWFDSMSTLATDIFLKGYQWPFDHDKIANFGSSLID
                     ILVQNEMVNRGRRVFMDFRENPRATQGLEAFKLADLKPEALTYLQKSGAIQATPIERL
                     AHMNQPSIDLYRQMGVDLWKEPLEIGVCSQHCNGGFAVDVWWESTVPHLFVIGELAGT
                     HGVKRPGGSALNAGQVGGTRAAQRIAHVYHGGGLPLDEFVGRAQAVVLRFVTEIGRIR
                     AAGTQTSDCAEVKKQIQRRMSRYAGMVRSLAGVSRALEEARSQWRQIRDHGLRQETAG
                     YAQAIEVRELALAQLGFLEAILALLKRGSGSRGSHLVTDPKGALPHPKLGDEWRYIPE
                     NLALRNEIMGVIYHPENDAFETRVSKPHGRPERESWFENTWTEYRRATIFVRDPGDHP
                     RPCKIYQE"
     gene            2853..3542
                     /locus_tag="P5316_15425"
     CDS             2853..3542
                     /locus_tag="P5316_15425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRR86398.1"
                     /translation="MAFKRIVELSYPIIPGQAGRKFDVEKIDADAVAPVPRLPGQWYI
                     MHNVVMVNHLGTHIEVPYHLRKDGLDLTEFPVENTIGTTRLLDVGHPPPGHGLTLEEI
                     KKAAILAGGVQQGEIVFLRTGWDKAWGTDEYLKSPWVRPEALAWLVRQGMVMFGIDAA
                     GVEELSNTKHESHYALFDHNVALIENLCNLDSLGKRKTFMSVCTPIAVKGLEAFPIRV
                     LGILNYGQFQV"
     gene            3523..4281
                     /locus_tag="P5316_15430"
     CDS             3523..4281
                     /locus_tag="P5316_15430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR86399.1"
                     /translation="MANSRFDGKVVIVTGGARGIGRAIVESFAREGAKVGIGHSGRSE
                     DAAQQIIRNIGKDRAVSLPGDVADAGAVKAILDKTISTFGRLDILVNNAGICPFRDVL
                     DVTVEEFDRVQAVNTRSIFLMSQGAARFMKDHGGGVIVNITSISGERFTDPQQLAYCT
                     SKAAANMLTRGLAVTLAPYGIRVNAVLPGTIPTDINQDVLARPGVADAIIRQTPLKCL
                     GECSDVAAATLFLAGDEAKWITGTLMVVDGGVIA"
     gene            complement(4463..5284)
                     /locus_tag="P5316_15435"
     CDS             complement(4463..5284)
                     /locus_tag="P5316_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HRR86400.1"
                     /translation="MSQVAVVNRLAFGSRLPGCPVRVKAFTLIEVLVVVAIIALLVGI
                     LIPALSKAREQARRSVCASNQKQIMTGVHMYAMQYAGQIPFSVPWFNASLTWIVRQEY
                     YNPRGYVHLGVLYGSRQIKDPAIFYCPSNKEFPHIYPEGWQNFSAGGGVERVATGYMY
                     AIAGQIDRYPKGERLHARLDSLKQESLVSCMFISKRDKRQQRGVWPHRGGVIAGYVDG
                     SARLNQVADAIAQTSAELYDANNIDDMDYFAFCFFKMLSGQRRWIEAFPKLPEVR"
     gene            complement(5575..6591)
                     /locus_tag="P5316_15440"
     CDS             complement(5575..6591)
                     /locus_tag="P5316_15440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRR86401.1"
                     /translation="MADNATSQDSTPVPKSRRRLRPVRLFVIAAVCVVAAFAVKHFWF
                     SLPIGEGPAGPVVPREMFQAVWTSRPVLLVGIGDSITDGFGASEGHSYFDMLISNPAD
                     ESPEMKGICLGSVLPNLKSLELAVSGSTSIHHLTSQLARLEVQPPEVLGLVVMTTGGN
                     DIIHNYGHSPPQEGAMYGATFEQARPWIAAFDERLKSMLDRIEQCFPGGCHIFLANIY
                     DPTDGVGDATRAGLPAWPDAMKVLAAYNEIIARHAGQRPNVHLVDIHSEFLGHGIYCT
                     QPWRRHYRWADPHYWYYMNLEDPNDRGYDAIRRLFLLEIAKVFQTTAPQSSPTPLNLN
                     HNGK"
     gene            7554..8555
                     /locus_tag="P5316_15445"
     CDS             7554..8555
                     /locus_tag="P5316_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86402.1"
                     /translation="MQILFGLAGLLVTARRRGLSSRTRFIDLQNTGVLYTIQVARKHM
                     VVELPGTGIDGEAGLAATFARNAVDLVRKLTRRLGMHTSRWIFGTVILFAATASLEAA
                     YISYEGDVFPENDGWIPGGSQEDPAVYTRSLDSGIFAINATATGTATVFYRRDLQPTS
                     DYVCIEWQAKTSNDDGYSYFSFLIDGDGLTSSIGLSWRASGAVIGMARDAGQLDDIVV
                     GTPLSPDEYHVFRVESSGSLFAVYVDGLFQVSNALQSPIGTNLRAQFGFTKDWDLVPT
                     TGSWDYIRAWDVPEPCASIMMTLILLAFAARRSCRIGEQTLAPGRGLQMLRGPAPSP"
     gene            8764..9465
                     /locus_tag="P5316_15450"
     CDS             8764..9465
                     /locus_tag="P5316_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86403.1"
                     /translation="MYRSHWILGTFILFAATASLEAAYIIYEGDVFPENDGWIPGGSQ
                     EDPAVYTRSLANGVFRIDATPLGGATVFYRKDVELTTDTVAIEWRARTSNDDGYSYQD
                     LLIGGEDLTSDISISWRSSNAVISLARDVGGLDRTVVGTPLLTSEFHTFRIESFGALF
                     SVYIDGELQVSDTLQAPTGTNLWTQFGFGKDWYYVPTAAEWDYIRIDEVPEPSALLVL
                     VIGLFMAVGRRCSRQ"
     gene            9494..>10304
                     /locus_tag="P5316_15455"
     CDS             9494..>10304
                     /locus_tag="P5316_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86404.1"
                     /translation="MRPRSFAAICVLAALTGDTIANGACGTIVVVRNGLRQTNQSTVD
                     CLPEWRFNELSTEVEVTETTVKFLGGCWSYLDGYHSQRAYRIYCASRQDITVTYSTVP
                     EHYQQIINRHVCIEFMYPSGSDPVPITNLTVNLSAFKSSQIAVGSRVSGDVSYIGLSR
                     GPGTPTYGGYCRLNLYSDTQQGITGSVNGNINCHDCYLDIAGSILSSANIGCGGPFDG
                     SITCGGSILGHITAASATEGSGTITAAGTITGANIGIANFAGNICSPGLTED"
     assembly_gap    10305..10314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10315..>11370
                     /locus_tag="P5316_15460"
     CDS             <10315..>11370
                     /locus_tag="P5316_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR86405.1"
                     /translation="DLPNVQPECGWKVCSQWVIGDDTDGDGYHNCEEECDNDPNKTEP
                     GVCGCGVPDTDSDGDGTANCIDGCPNDPDKTEPGVCGCGVPDTDVDMDSMLDCEDNCP
                     TVYNPDQTDTDGDDSGNACDPDDDEDGIDDPIDNCPLISNSTQTDTDGDGVGDACDNC
                     LNVLNPDQRDTDRDGIGDVCENVIYVKWDASGLNNGTSWTNAYTSLQAALAAAEAAVN
                     LASNPPVKPDIWVAASPNPTDYYKPSVPSGRAATFTLKNNVSVYGGFAGTEEPATFRL
                     ADRDFTTKQTVLSGDIGTPGSNADNCYHVVTGSNTDSTALLDGFTITAGNANGSATSE
                     KRGGGLYLAAGSPAIVN"
BASE COUNT         2291 a         3519 c         3351 g         2199 t
ORIGIN      
        1 tggccggctc gacctgctgg ttaacaacgc aggcgtcgcc cccaagcagc gagtcgacat
       61 cctccaggcc ggcgaggaaa ccttcgaccg gctcatctcg atcaatctca aggggccgta
      121 ttttctcacc cagcttgcag ccaaccggat gattgcctgg aaaaaggccg gtcggatcac
      181 cacgccacgg attgtgttca tcacgtcgat ctcggcgtac acgtcgtcgc cggcccgggg
      241 cgaatactgc gtctccaagg ccggcctctc catggctgtc cggttatacg ccgatcgtct
      301 cggcgaacac ggtatcccgg tcatcgagat acgtcccggc atcatccaga cgccgatgac
      361 cagcgtggtc aaggacaagt acgacaagct catcgccgag ggtttgctga tcaaccgacg
      421 atggggcttg ccggaggacg tcgccgccgt ggttgctgcg gttgcccgcg gcgatctgga
      481 ctactccacc ggcgagagca tcgaggtgag cggcggattc aacgtgcgga gactgtgaga
      541 gacaccatga ttccgagaag cagtgctgat ttgaacggtc tgagcatccc cgtctactcg
      601 ctcaacacgg tgatcgtcgg cagtggcgca gccggcctca actgcgcctg ccggctgttc
      661 agggaacttg aggagatggg catcgagaat ccggccgatc acctcgcctt ggtcactcgc
      721 ggaatcggac tgggcacctc aaacaactcc ggttccgaca aacagaccta ttacaaaatg
      781 ggaaccggcg gggagcccga tcgtccgacc gacttcgccg atacgctgac ggccggaggt
      841 tgcactcacg cagacgtcgc attcatcgaa ggagccaact cgctccgcgc tttctacagg
      901 ctggtcgact tgggtgttcc ttttccccac accgccgacg gcgttttcgt gggttacaag
      961 accgaccatg acccgcgaca acgcgcgact tccgccggcc cgtggacttc gcgattcatg
     1021 gtccgcaagc tgctcgccga actggagcgc tacggcatcc ccgttttcaa tcgccatcac
     1081 atgctcgcgg tcgtcaccgg acgggaagaa ggtcgacagg ccgcctgcgg cattctctgc
     1141 gtcgacctcg cccgccagaa cgacgacaat cacggcctgg tgctgttcaa cgcccgcaat
     1201 gtcgtggctg ccggcggcgg gcccggagat ttgtacgaga tctccgtcta tcccccggga
     1261 cagatgggac cctatgccgc cctgttcgag gccggcgcgg aggctcataa tctcactgag
     1321 tcgcaattcg gcctcgcctc gctgcgcccg cgatggaacc tctcaggaac ctatcagcag
     1381 gtcatccccc gttactacag caccgacgcc gacggcgagg atgagcagga gtttcttaac
     1441 ccctggttcg actccatgtc gacgctcgcc accgacattt tcctcaaggg ctaccagtgg
     1501 cccttcgacc acgacaagat cgccaatttc ggctcgtcgc tgatcgatat cctcgttcag
     1561 aatgagatgg tcaaccgcgg ccgccgcgtc ttcatggatt tccgcgagaa cccgcgtgcc
     1621 acccaagggc ttgaggcctt caagctcgcc gacctcaagc cggaggccct cacttacctg
     1681 cagaagtccg gggccataca agccaccccg atcgagcggc tggcacacat gaatcagccg
     1741 agcatcgatc tctaccgaca gatgggcgtc gacttgtgga aggaaccgct cgaaatcgga
     1801 gtctgctcgc agcactgcaa cggcggtttc gcggttgacg tctggtggga atccaccgta
     1861 cctcatcttt tcgtcatcgg tgagctggcc ggcacgcacg gggtcaaacg gcccggcggc
     1921 tcggcgctca acgccggtca ggtcggcggg acccgggccg ctcagcgcat cgcgcacgtc
     1981 taccacggcg gtggcctgcc gctggacgaa ttcgttggcc gtgctcaagc cgtcgtcctt
     2041 cgttttgtca ccgagatcgg tcgcattcga gcggcaggta cccagacctc ggactgcgcc
     2101 gaggttaaga aacagattca acgccgcatg agccgatacg ccggcatggt ccgctcgctg
     2161 gccggcgtga gcagggctct ggaagaagct cgcagccagt ggcgccagat ccgagatcac
     2221 ggcttgcggc aggaaaccgc cggctacgcc caagccatcg aagtccgcga gttggccctg
     2281 gcacaactgg gtttcctgga agcgatcctt gcgttgctca agcgcggcag cggctctcgc
     2341 gggtcgcacc ttgtgaccga ccccaaaggc gccctgccgc atccgaagct cggggatgag
     2401 tggcgctaca tccccgaaaa ccttgccctc cgaaacgaga tcatgggcgt gatttaccat
     2461 cccgaaaacg acgccttcga gacgcgcgtc agcaagcccc acggacgtcc tgaacgcgaa
     2521 tcctggttcg agaacacctg gaccgaatac cgccgtgcga ccatcttcgt acgcgatccg
     2581 ggcgaccacc ccagaccgtg caagatctat caggagtaac cgacacgagc tcgcggtcgg
     2641 gggcaaccta tgcgccggcc gcaggccgtt cacccgcggg aagccttcgc cggccactca
     2701 accacacaca ggcacgcggc ggccttgcga ccctcgcagc gtcagatcaa gaccactgaa
     2761 catccggacg ttgcggagac ggctcgttgc gatatagtgg ggctccggtt ttgagtccgg
     2821 acgaaccaag tgcatcggcg aggaaacagc cgttggcttt caagcgtatc gtcgaattgt
     2881 cgtacccgat catccccggc caagcggggc gcaaattcga cgtcgagaag atcgacgccg
     2941 acgccgtcgc ccccgttcca cggctgcccg gccagtggta catcatgcac aacgtggtca
     3001 tggtcaacca cctcggcacg cacatcgagg ttccctacca cctccgcaag gacggcttgg
     3061 acctgaccga gttcccggtg gagaacacga tcggaacgac ccgcctgctc gacgtaggcc
     3121 atccgccgcc cggccacggc ctgacgctcg aggagatcaa gaaggccgcg atcctggccg
     3181 gcggcgttca gcagggcgaa atcgtgttcc tgcgcaccgg ctgggacaag gcttggggaa
     3241 ccgacgagta cctcaagagc ccctgggtac gtcccgaagc cctggcttgg ctcgtccgcc
     3301 aaggcatggt catgtttgga attgatgccg ccggcgtcga agagttgagc aacacgaaac
     3361 acgagtcgca ttacgccctc ttcgaccaca acgtcgccct gatcgagaac ctctgcaatc
     3421 tggattctct cggcaagcgc aagacgttca tgagcgtctg cacgccgatc gcggtcaaag
     3481 gtctcgaagc gtttccgatt cgtgtactgg ggattctcaa ctatggccaa ttccaggttt
     3541 gacggcaaag tggtcatcgt aaccggcggg gcccgcggca tcggcagggc catcgtcgaa
     3601 tcatttgccc gcgagggagc caaggtgggc atcggtcatt ccggacgctc ggaggacgcc
     3661 gctcagcaga tcatccgaaa catcggcaag gatcgagccg tctcactgcc cggcgacgtg
     3721 gccgatgccg gggcggtcaa ggccatcctt gacaagacga tctccacctt cggccgcctc
     3781 gacatcctcg tgaacaacgc gggaatctgc ccgtttcgag acgttctgga tgtgaccgtc
     3841 gaggagttcg atcgcgttca agcggtcaac acccgcagca ttttcctgat gtcccagggt
     3901 gcggcccgct tcatgaagga tcacggcggc ggggtcatcg tcaatatcac cagcatcagc
     3961 ggcgagcggt tcaccgatcc gcagcaattg gcctattgca cgagcaaggc ggcggccaac
     4021 atgctcaccc gtggcctggc cgtcaccttg gccccctacg ggatccgggt caacgccgtg
     4081 ctgcccggca ccattcccac cgacatcaac caggacgtcc tcgcccggcc gggcgttgcc
     4141 gacgccatca tcaggcagac gcctctcaag tgcctcggtg agtgcagcga cgtcgcggcg
     4201 gccactctgt tcctggccgg cgacgaagcc aagtggatca caggtacgct catggtcgtc
     4261 gatggcggcg tcatcgcata ggtgtgcgcg acgagatgag gcagggcagc gtcgaggtcg
     4321 ccgccgcgac caagacctgc cgtctttact gagacaccac cacctgtgcg tttcgccgtt
     4381 cgcgacggaa caatgcccat tctcagttct gcattcgcca ttcttcattc tccagcattg
     4441 ccgtacgcaa cgctggaggc tactaccgta cttccggcag cttggggaac gcttcaatcc
     4501 agcgacgctg accgctgagc atcttgaaga agcagaaggc aaagtaatcc atgtcgtcga
     4561 tgttgttcgc gtcatagagc tcggccgacg tctgagcgat cgcatcggcc acttggttga
     4621 ggcgggcgct gccgtcgacg tagccggcaa tgaccccgcc gcgatggggc cacactcctc
     4681 gctgttgccg cttgtcacgc ttcgagatga acatgcagga caccagtgat tcctgcttga
     4741 gtgagtcgag gcgggcgtgc agccgctctc ccttggggta acggtcgatc tgccctgcaa
     4801 tcgcgtacat gtacccggtc gcgacacgct cgacgccgcc cccggccgag aagttctgcc
     4861 aaccttccgg gtagatgtgc ggaaactcct tgttcgacgg gcagtagaag atcgccggat
     4921 ccttgatctg ccggctgccg tagagcacgc ccaggtgaac ataacctctc gggttgtaat
     4981 actcctgccg gacgatccag gtaaggctgg cattgaacca cggcacgctg aacgggattt
     5041 gaccggcgta ctgcattgcg tacatgtgca ctccggtcat gatctgcttc tggttgctcg
     5101 cacacaccga ccgccgggcc tgctcacggg ccttcgacaa ggcggggatc agaatcccca
     5161 ccagcaaggc aataatcgcg acgaccacca gaacctcaat aagcgtgaag gctttgaccc
     5221 tcactgggca acctggcaac cgtgaaccga aagcgagtct gttgacgact gccacctgcg
     5281 acatgctcga ctccccgtgt gcccaagaac caacacggct catcaacttg gcgactctcg
     5341 ccgggacgag agtctctacg cccccttgtc gaccaaactt attgtattct gtaaacaagc
     5401 gattggcaag cccggctcat tgctcgcaca gggcgcctgt ctccgagtga ctggcgggtt
     5461 ccgtacgccc ggtgtcgttg aaatcagccg cgatgtgcca gttgaacaag ccattgagga
     5521 actactgctt ctggcagagc gcgctttgca cgaggaaatc gaagaacgcg tgctctattt
     5581 gccgttgtga ttgaggttga gcggagtagg actcgattgg ggcgcggtcg tctgaaacac
     5641 cttggcaatc tccagcagaa acagtcgacg gatcgcgtcg tagccccggt cgttggggtc
     5701 ctccaggttc atgtaatacc agtagtgagg gtcagcccag cgatagtggc gccgccacgg
     5761 ctgcgtgcag tagatgccat ggccgagaaa ctcgctgtgg atgtccacca gatgcacgtt
     5821 cggtcgctgc ccggcatggc gggcgatgat ctcgttgtat gccgcgagaa ccttcatcgc
     5881 gtcgggccag gcgggcaaac cggctcgcgt ggcgtcacca accccgtcgg tcgggtcgta
     5941 gatgttggcc agaaagatgt gacagccgcc cggaaagcac tgctcgatcc gatcgagcat
     6001 gctcttgagc cgctcatcaa atgccgcgat ccaggggcgg gcctgctcga aagtggcgcc
     6061 gtacatcgcc ccctcctgag gggggctgtg accgtaattg tggatgatgt cgttgccgcc
     6121 cgtggtcatg accaccaggc ccagtacctc cggcggctgc acctccaggc gggcaagctg
     6181 gctcgtcaga tggtggatgg acgtgcttcc ggagaccgcc agctcaagcg atttcagatt
     6241 cggcaacacg ctgccgagac agatcccctt catctccggg gactcgtcgg ccgggttcga
     6301 gatcagcatg tcgaagtagg aatgcccctc ggatgcccca aaaccgtcgg tgatgctgtc
     6361 gccgatcccc accagaagaa ccggccgcga cgtccagacg gcctggaaca tctccctcgg
     6421 aacgaccggc cccgccggtc cctcgccgat cggcaagctg aaccagaagt gcttgaccgc
     6481 aaatgcggca acaacgcaga ctgccgcgat gacaaacaac cgcaccgggc ggagccgcct
     6541 ccggcttttg gggacgggag tggagtcctg actcgtggcg ttgtctgcca ttcaagtcct
     6601 cttggctgat tggctggaga gattcgcagc ctcctgccgc ctgtggatga tgcgctttga
     6661 gagtctatga agtccacctc gctgccttgc cccccagtca cacaaagatc gtcgcaggtc
     6721 tgcaggactg gggtatgtct ttgactcaac ctaacgctgt tttcgcggcc aatatcgagc
     6781 aagggctggg acagttgtgc aggattccgc ccaaacccga taggtggttg agtcggatgc
     6841 ataccccaat tgcaggaaac cccttgtggg tcggacgtct cgcgccgaat tcctccttgg
     6901 gcattcattg tggtgccgac gccctcgtcg gcacgtctga cacatcgctg gccccagaat
     6961 cctttctggg gccttctagt tgcccctgct ccgctggccc cagaatccct tctggtgccc
     7021 acatcgctgg ccccagaatc ctttctgggg ccttctagtt gcccctgctc cggaatccgt
     7081 gtgcggaacg gaacacccgg agtgttatcc ctggagttgg aggctccatg actctgcgat
     7141 cggttcgcaa agggcgtgtg ggcgcgatga caccgcagcg aacaacggca aaatgcgaaa
     7201 cgaacccaag gatgcaagcg atcagccatc agcgttccgc gttcgcccga tgagaagttc
     7261 accaggcgcg tctggctgac cgctgagtgc cgaatgctga caacctctcg cgccaaagga
     7321 agccaaactt gggcaaacca gcgaggaccg gcaagctgtt gaggcgggaa ccctgcgaat
     7381 ctgggggata tgctgccgga tggacaagca tcgaggtaca agcgaatgct cgacggtccg
     7441 tcgctggtcc gttcgcccgc gatcgccgtg ttacgccctc ctcatatcct tcgcggacaa
     7501 ctgagggcag cagaaaagtg gaatgtcccc tttgtttcct ccccgagcca tcgatgcaga
     7561 ttctgttcgg attggcgggt cttctggtga ccgcccggcg tcgtggcctt tcttcgcgca
     7621 ctcgtttcat tgacttgcag aacactgggg ttctgtatac gatccaagta gctcgcaagc
     7681 acatggtggt ggagttgccc ggaaccggga ttgacggaga agcgggtctt gccgctacat
     7741 tcgcgaggaa cgccgttgat ctcgtccgga agctcacaag gaggttgggg atgcacacgt
     7801 cacgctggat attcggcaca gtcattctct tcgcggccac tgcttcgctc gaagcagcct
     7861 atatcagtta tgaaggcgac gtgtttccgg agaacgacgg ctggattccg ggagggagcc
     7921 aggaagaccc tgccgtctac acccgttcgc ttgattcagg catctttgcg atcaacgcaa
     7981 ctgcgacggg aacggccact gtattctacc gcagagatct acagccgacg agcgactatg
     8041 tctgtatcga atggcaggcg aaaacatcca atgatgacgg ttactcctat tttagctttc
     8101 ttattgacgg cgacggtctt accagcagca ttggtctttc atggcgagcc tccggggcag
     8161 tgatcgggat ggcaagagat gctggccaac ttgatgacat agttgttgga acacctttgt
     8221 cgcctgatga atatcatgtc ttccgcgtcg agtcatccgg atcccttttt gccgtctacg
     8281 tcgacggact gttccaggtc agcaacgctc tccagtcacc catcgggacc aacctgcgcg
     8341 cgcagttcgg attcacgaaa gactgggacc ttgtgccaac cactggttca tgggactaca
     8401 ttcgagcatg ggatgtccct gagccttgcg cctcgatcat gatgaccttg atccttttgg
     8461 catttgctgc tcgtcgctct tgcagaatag gagaacagac gcttgcccct gggagaggat
     8521 tgcagatgct ccgaggcccg gcgccttctc catgagcaag gccgtctcag cggttgttct
     8581 ctatcgctag cattcctttc aaaggcttgc agaagattcg ggttctggat acgatgcagg
     8641 tgactcgcgg gcgcagagtg gcggagttgc gcggaacagg gagtagggac aaaccggatt
     8701 tcgccactat gctccccaag cggcgttggc aatccggtcc agaagctcat gaggaggatc
     8761 gcgatgtaca ggtcacactg gatactcggc acattcattc tcttcgcggc tactgcttcg
     8821 cttgaggcag cctatatcat ctatgaaggt gacgtgtttc cggagaacga cggctggatt
     8881 ccgggaggga gccaggaaga ccccgctgtc tacacccgtt cgcttgccaa cggcgttttt
     8941 cgtattgacg ctacaccgct gggcggcgcg acggtgttct accgcaagga cgtggaactg
     9001 actacggaca ccgtcgcgat cgaatggcgg gcaagaacgt ccaatgacga tggctactcg
     9061 tatcaggatc ttctcatcgg tggcgaggat ctgaccagtg acatcagcat ttcctggcgg
     9121 tcctccaatg ctgtaatcag cctggccagg gacgtgggag gactcgatcg tacagtggta
     9181 ggaacgcccc tgctgacaag tgagtttcac acctttcgca tcgagtcgtt tggcgcattg
     9241 ttctccgttt acatcgacgg cgagcttcag gttagcgata ctctccaggc tcccactggg
     9301 accaacttgt ggacgcagtt tggttttggc aaggactggt actacgtccc aaccgctgct
     9361 gaatgggact acatccgcat cgacgaagtg cccgagccat cagcattgct ggtgttggtg
     9421 atcggcttgt tcatggccgt cggccgccgc tgctcgcggc agtaggaagt ttcttgtcag
     9481 gaggagaata gacgtgaggc cacgcagttt cgcagctata tgtgtacttg ccgctctgac
     9541 cggcgacacg attgccaacg gtgcctgtgg cacgatcgtt gtcgtccgca acggccttcg
     9601 gcaaaccaat caatctactg tcgattgtct ccccgaatgg cgattcaatg agctgtccac
     9661 tgaagtcgag gtaacggaga caacggtgaa gttcttggga ggatgctgga gctatcttga
     9721 cggctaccat tcgcagcgag cgtatcgcat ctactgtgcc agtcgtcagg atatcacggt
     9781 cacttattcg actgttccag aacactacca gcagatcatc aaccggcacg tctgcatcga
     9841 attcatgtat ccctcaggat cagacccggt cccgatcacc aacctcacgg tcaatctgtc
     9901 cgcattcaag agctcccaga tcgccgttgg ctctcgggtt tccggagacg tgtcctacat
     9961 cggcttgtct cgcggccccg gaacacccac ctatggaggc tactgcagac tcaacctcta
    10021 cagcgatacg cagcagggta tcaccggtag tgtgaatgga aacatcaact gccatgactg
    10081 ctatctcgat atcgccggca gcatcttgag cagtgccaac attggctgtg gtggcccatt
    10141 cgacggctcc attacctgtg gcggaagcat cctcggccac atcactgcgg cgagcgccac
    10201 ggagggctcc ggcaccataa cggcggccgg gactatcacc ggagccaata tcggcattgc
    10261 gaacttcgcc ggcaacatct gttcgcccgg actgaccgaa gacgnnnnnn nnnncgattt
    10321 gcccaatgtg cagccggagt gtggatggaa ggtctgcagt cagtgggtca tcggcgatga
    10381 tacggacggc gacgggtacc acaactgcga ggaggaatgc gacaacgatc cgaacaagac
    10441 tgagccgggc gtatgtggct gcggcgtgcc ggataccgat tccgacggcg acgggacagc
    10501 gaattgcatc gacggttgcc ccaatgaccc ggacaaaaca gagccaggcg tgtgcggatg
    10561 cggcgtaccg gacaccgacg tcgacatgga cagcatgctg gactgcgagg acaattgccc
    10621 gaccgtgtac aacccggacc agaccgacac ggacggtgac gactcgggaa atgcttgtga
    10681 cccagacgac gacgaagacg gcatcgacga tccgatcgac aactgtcccc tcatctcaaa
    10741 ctcgactcaa acggataccg acggtgacgg cgtgggcgac gcctgcgaca actgtctcaa
    10801 cgtcctcaac cccgaccaac gggacaccga ccgtgacggt atcggcgacg tctgcgagaa
    10861 cgtgatctac gtcaagtggg acgcgagtgg actgaacaac ggcacaagct ggaccaatgc
    10921 ctacacgtcg ttgcaggccg ccctggccgc ggccgaggct gcggtgaact tggcctctaa
    10981 cccgccggtg aaacctgaca tctgggtggc cgcgtccccg aacccgacgg actactacaa
    11041 gcccagtgtg ccgagtggac gggcagcgac tttcacgttg aagaacaacg tctcggtcta
    11101 cggcggtttt gccggcaccg aagagccggc cacgttcaga ctggccgatc gcgacttcac
    11161 gacgaagcag acggttctca gcggtgatat cggcacgccc ggcagcaatg cagacaattg
    11221 ttatcacgtc gttaccggca gcaacacgga cagcacggct ctcctggatg gcttcacgat
    11281 cacggccggc aacgcgaatg gttccgcaac cagcgagaag cgcgggggcg ggctgtatct
    11341 ggcggccggc agccccgcga ttgtgaactg
//
LOCUS       DAOTPW010000182        11289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5434_length_11289_cov_5.535784, whole genome shotgun sequence.
ACCESSION   DAOTPW010000182 DAOTPW010000000
VERSION     DAOTPW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11289
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5434_length_11289_cov_5.535784"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            558..1334
                     /locus_tag="P5316_15465"
     CDS             558..1334
                     /locus_tag="P5316_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86406.1"
                     /translation="MRNAQAFLILAGALNDAGHFSNGMNLYAYLGANAVNSTDPSGLK
                     EHHIWPLHLGGPSDGPGIDLDDASHQAFHDYLSKKGFPFGDKGRAKWAALSDAERRGY
                     ICDAARAAGVDVTDKGFQKALKEAAKEANAIIRKAKGADFLQPRAACRYVLRLEKAEN
                     GVADKLVRRVSGGRLDKLGAIGKGMAVVGGVMYGMQMMDSLRIDNPYVWELAVISRRI
                     QNTGQMTIMDEAAAADDLYHLTGDCFSGAYAWNYWRDTVW"
     gene            1356..2681
                     /locus_tag="P5316_15470"
     CDS             1356..2681
                     /locus_tag="P5316_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86407.1"
                     /translation="MDDLKLGPPLDRRFLRKLASALIEDGWSDLSDMVGWLTRHGSGE
                     AMAAAICDAWSGTSLPLRDWAAQVAGVEVWLRGNDLRIAVRVSGDLETTVWQAAPFEL
                     TAFEAMTAALPRPLPKLLRDAALSAPGMVLDRVGPLADPWYLAKPLAYVTRWTEGTNR
                     EECERLGFEYPDPNRHLIEVVDNGLGDCYVLGTDGTMSFFDHEARGLARCNITLEGLL
                     HQYFASPQGILDPYERKWAERPREMPSAEPWPDPPSVVLSLKVQGLPFLASIALAVRG
                     CQRAMPREWLRATPQEWQSFQDTLGQIIERCKDVATTGTPLSRQAAKDARRTADIVTA
                     KWKRLCGIGGKVYPFEQLTYFAVPALATACHRISDAEAIEEAVASCQAVYTGSDRQDE
                     TVTQAAIGDADYLLLLNLGEPATVGKPVPSAFFERPLWPGGSPREIWNG"
     gene            3052..3273
                     /locus_tag="P5316_15475"
     CDS             3052..3273
                     /locus_tag="P5316_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86408.1"
                     /translation="MDENTTPAVSYTWDTSHYGENGYNYDRLVGMTYPTRPETGARSL
                     WEIGGHYTYFDEIGAVFRCKLRRSAWHNR"
     gene            3336..3572
                     /locus_tag="P5316_15480"
     CDS             3336..3572
                     /locus_tag="P5316_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86409.1"
                     /translation="MLLTETMHRQYHGGLLRRLSQKGIYPKRGQSWLKYFEENAGKQQ
                     AALDVMVDYTREFDSANGTGLLKHVLQQLSAQGR"
     gene            3581..4603
                     /locus_tag="P5316_15485"
     CDS             3581..4603
                     /locus_tag="P5316_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86410.1"
                     /translation="MCKQGMIVELVGKKKDAYTHPVVQWAKEQRGRILCPNCGKLRQD
                     LFPQPVDVPLIQLYKGTSYSIVFRGGVAVIHQRLLDFLRPHLPEHSLGRCFWRDGSLL
                     PDYHSIYFRDYIFFRAGRESPRYEYYECEICGLSGASSDEPYVLRSELPDATVFQDAI
                     HCLYLSASLARVFPWREFRDLEPFTFPVRDEPLPDDPLPVGWRPAFKGQAWLTARTNS
                     LPESVREPEPDRYAELKAMTLDLPRLEDDSAKSCPVPMRKIMSYLRLEGVDRRIKARD
                     LQFLRTARVGDRDCWIWRFEEQVCADAYVTVWRGPDGALCVGYGGNSHGLTPEQYIYG
                     EHHKYF"
     gene            5560..7206
                     /locus_tag="P5316_15490"
     CDS             5560..7206
                     /locus_tag="P5316_15490"
                     /inference="COORDINATES: protein motif:HMM:NF020291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HRR86411.1"
                     /translation="MPEGQRDATQARLAGAMRRGGMSQREILAALQVTHQARCQPPLP
                     DDEVERIAASVARYAPDAVSVALIEDHYAQIFGTGHDTKPVGESSPAVDPGPLPDELF
                     RVPGFIGEVMDFCLETAPYPNLALAFGGALALQAFLAGRKVRDAADNRTNLYVLALAN
                     SGAGKNEPRKVNQRICVEVGLQDCLGDAFASGEGIEDRLFVTPSVLFQTDEIDGLMNA
                     INKASDARHEGIMNVLLKMYTSSSTIHPMRVKAGGQSPGVIDQPSLCMLGTAVPKYYY
                     EALNVRMLNNGFFARLIILEAGKRGRGQTPVARPIPQSILDTARWWADYRPATGNLQD
                     WHPIPVCVEATPEADAAMEECRAYADDRYAEAEEKDDPAAMAIWARACEKARKLALVH
                     AVSADHLQPRIDERAVQWAWRFVDYQTRRMLFMAGQHVAEGEFDAKCKRMLEVLTSWR
                     QRRGDAWMPHWELSRRLKWSDKDIEEVRNALRGQRRIEHDVGSTAGGGPVAQRYRLLC
                     PGGQLPQLPHVNVATGGANESVADQTAGSPHEPMRELAGT"
     gene            complement(7411..8634)
                     /locus_tag="P5316_15495"
     CDS             complement(7411..8634)
                     /locus_tag="P5316_15495"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR86412.1"
                     /translation="MRSRYLAEAVKRLCFARRHKIALVSGPRQCGKTTMAKMLLAERA
                     VGGYHNWDEFQFRHAWAKQPSVVLPPGKGRAVPLVVLDEIHKDRLWKRNLKGLYDTLE
                     RPCDFLVTGSARLNIYRRGSDSLLGRYFHFRLHPFSLREMEDVTFLSPDGALEALFGR
                     AFKPGRSAEDRLPALMTYGPFPEPLFEHDERAARLWRRNHQQIVIREDLRDISRLPDL
                     GRIELLAALLPERVGSLLSVASLRETMEVSFDTVRRWLMYLKEVYYLFEVKPWSRRIP
                     RSLRREGKVYLWDWAAVPTEPARFENLVASHLLKACHYWTDTGEGEFDLFYLRDKERR
                     EIDFLIVRDGKPWLPVEVKHADVEPSPNWGRFAPLLGCKHGLQVVRQPAWRVHCCGDT
                     KVLVAGASEALRYFA"
     gene            9096..10466
                     /locus_tag="P5316_15500"
     CDS             9096..10466
                     /locus_tag="P5316_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HRR86413.1"
                     /translation="MNIELRPLAQIKPYENNPRINDAAVDAVAESIRRFGFRQPIVVD
                     ADGVIVCGHTRWKAAQKLGLEQVPVHVAKDLTPEQIRAYRIADNKTAELAEWNLELLP
                     IELAELQGAGIDWSLLGFDGDELAKLLDPGVKQGLTDPDEVPEPPDEAITQPGDIWVL
                     GDHRLMCGDAARAEDVDRLLDGRPIHLVNTDPPYNVKVEPRSNNAIAAGLSSFGEAGV
                     TNHQGFDLARRPDVARPTHRKLRPKDRLLANDFVSDEEFERLLDAWFSQLARVLEPGR
                     AFYIWGGYANCGNYPPFLKKHGLYFSQAIIWDKQHPVLTRKDYMGAHEWCFYGWREGA
                     AHVFLGPNNATDLWHVKKINPQSMIHLTEKPVELAVRAMQYSSRVGENVLDLFGGSGS
                     TLIAAEQTARRAYLMELDPLYCDVIVERWSKFTGRNAERISGQSNAPVEAEALEVEAS
                     CASDQA"
     gene            complement(10457..>11289)
                     /locus_tag="P5316_15505"
     CDS             complement(10457..>11289)
                     /locus_tag="P5316_15505"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRR86414.1"
                     /translation="NSHGGWNATNTATGKRIRIKSPQRLRHKVSPKNGGKPPAANAAV
                     VETTAPPSIVPAEAAQSEAEEDRPGYDPARCATEGCKGTPVMTYLDRPLCQACWERHC
                     EEQEPSEVGENTIDPSACDAQAEACHPIGEPQTPEQVKAMPKSKSTNKQSEAQKRDDR
                     DLDKAPRSELTLEEAERLAKVRAAKAKKAAADQPKRLSALDAAAEVLKTEGKPMRAKE
                     LIEAMAAQGLWSSPGGKTPEATLYAAMSREIKKADENGTVSRFRKTDRGQFEYNDHHA
                     "
BASE COUNT         2101 a         3502 c         3628 g         2058 t
ORIGIN      
        1 gggcatgttc tttgacaaga gcatacgcga gattggcttg ccactggccg ggttgctttg
       61 cacgccgatc ccggctgcga ggcgaggagt tgttgcccgc agatggacgc agattcacgc
      121 agatgttgac gcggccggac acggccttgt gcggtgctga aatctgcgtc tatcccatca
      181 atctgcgggc cgagactccg agcactgcta ctgcgaccct cgacacgacg ggcgggcaat
      241 gcctgtgccc acggctcaga cgctgctgcc ccgccccgac tgaccgctga caactgactg
      301 ctgaccactg gccgccacag cggccccagt gggaacccta cggctccctg gccacgctcg
      361 acacaaccgc ctccccccgc cccgataact ccgtcgggca tcaaggcctt ttcttctact
      421 ccttcaacac cgctgacagc ttatcgctga acgctgcggg cttatatcac aaccgcaatc
      481 gctggtactc gcccgcgctg gggcggttca cgtcgagaga tccgattgag acggcgcagt
      541 tggtcgttag tgccgctgtg aggaacgcgc aagcgtttct gatcctggcc ggggccttga
      601 acgatgcagg gcacttctcg aatggaatga acctctatgc gtatcttggg gcgaatgcgg
      661 tcaatagtac cgatccatct ggtctgaagg agcaccacat ttggccgctg cacctgggag
      721 gaccgagtga cggtccgggg atcgatcttg atgacgccag ccaccaggcg tttcacgact
      781 acctcagcaa gaagggtttc ccgtttggcg acaagggtcg ggccaaatgg gccgcactga
      841 gcgatgcgga acggcggggg tatatctgcg atgcagcacg ggccgcaggc gtggacgtga
      901 cagataaggg cttccagaag gccctcaaag aagcggcgaa ggaggcaaac gcaatcatac
      961 gcaaagcgaa aggcgctgat tttctacaac cgcgagcagc atgcaggtac gtcttgcgac
     1021 tggaaaaggc cgagaacggg gtcgccgaca agctggtcag acgggtgtca ggcggacggc
     1081 tcgacaaact cggcgcaatc ggcaaaggaa tggctgtcgt cggcggtgtc atgtatggca
     1141 tgcagatgat ggatagcttg agaatagaca atccatatgt gtgggagctg gcagtgatct
     1201 cccgtaggat acagaatact ggccagatga cgatcatgga tgaggccgcg gctgctgacg
     1261 atctctacca tctgactggg gactgtttct ccggtgccta tgcgtggaac tactggcggg
     1321 atactgtctg gtgagcgtat cttggaggcg tgggcatgga tgatctaaaa cttgggcctc
     1381 cgctggatcg gcgtttcctg cggaaactcg catctgcttt gatcgaggac ggctggagtg
     1441 acctgagtga catggtcggc tggctgaccc ggcacgggtc gggcgaggcc atggcggccg
     1501 ccatttgcga tgcatggtcc ggcacgtcgc tgccgttgcg cgactgggct gctcaggttg
     1561 ccggcgtgga ggtatggctt cgagggaacg acctgagaat tgctgtgcgc gtctcgggcg
     1621 atctggagac aacagtatgg caagcagcgc cctttgagct gacggcgttt gaggccatga
     1681 cggcagcact tccgcgaccc ctgccgaagc tgctccggga tgcggcactt tccgctcccg
     1741 gcatggtcct cgaccgggtc ggcccgctgg ccgacccctg gtacttggcc aagcctcttg
     1801 catacgtgac aaggtggaca gaaggcacaa accgcgagga gtgcgagagg ctcggattcg
     1861 agtatcccga tcccaatcgc cacctaatcg aagtggtgga caatggactg ggggactgct
     1921 acgtgcttgg cacagatggc acgatgtcct tcttcgacca tgaggcccgc ggtctcgcga
     1981 ggtgcaatat cacgctggag gggctccttc atcagtactt cgcgtcaccg caaggaatcc
     2041 tggatccgta tgagcggaag tgggccgagc gaccacgcga gatgccctcc gctgaaccgt
     2101 ggccggaccc tccaagtgta gtgctctcat tgaaagtcca ggggttgccc tttttagcca
     2161 gcattgcgct agccgttcga ggctgccagc gggccatgcc tcgtgagtgg ctccgtgcca
     2221 ctccacagga gtggcagtcg tttcaggaca ctctcgggca gatcatcgaa cgctgcaagg
     2281 atgtggcgac aaccggtaca ccgcttagca ggcaagcggc gaaggatgcc cgccgtacgg
     2341 ccgacatcgt gaccgccaag tggaagagac tctgtggcat cggaggcaag gtgtacccat
     2401 tcgagcaact cacgtacttc gccgtcccgg ccctggcgac agcgtgccat cgcatcagcg
     2461 atgccgaggc catcgaggag gccgtagcgt cgtgccaagc agtgtatacc ggcagcgatc
     2521 gccaggatga gaccgttacc caagcggcaa tcggggacgc ggattacctc ctgttgctca
     2581 acctgggcga gccagccaca gtgggcaagc ctgttccttc tgcgtttttt gagcggccgc
     2641 tgtggcccgg cgggtcgcct cgcgagatat ggaacggctg accggaagga tgccggcagg
     2701 aacattgaca gagggcggcg tgagctcagt gcttcatgat tcagagtacg gaatggggcc
     2761 ggccggacgg atgagaaggg caaccggttc gtctaccact acgacgagca agggcggctc
     2821 gtccaaaccg acgtcaccgg ctcgcgctgg tacgcctcgg gggccaacga gcggtacgac
     2881 ccgccgccgc cgattaagac catcacctac agctacacgc ccgacggcct gcccgaaacc
     2941 gtcaccgcgt acgacggcca gaacgccacc ggcaacatcc tctcccagag ccatttccac
     3001 tatgacgggc tgcgccatct gacggccgag ttccagtctc acgggggtcc ggtggatgaa
     3061 aacaccaccc cggccgtcag ctatacctgg gatacaagcc attatggcga aaacggttac
     3121 aactacgacc ggctggtcgg catgacctac cccacacggc ccgagactgg tgcccgcagt
     3181 ctgtgggaaa tcgggggaca ctatacgtat ttcgatgaga taggggctgt tttccgatgc
     3241 aaactgcgaa gatcggcttg gcataatagg taaggtgtcc cccgaattcc cgtgctcggg
     3301 ccaagttccc acgagggttc gacgacggca tcaaggtgct gctcaccgag accatgcatc
     3361 ggcagtatca tggcggtttg ctgcgacgtt tgagccagaa gggcatctac ccgaagcgcg
     3421 ggcagtcgtg gctgaagtac ttcgaggaaa acgcgggcaa gcaacaggcg gcccttgatg
     3481 tgatggtcga ctacaccagg gagtttgaca gtgcgaacgg aacgggcctg ctcaagcatg
     3541 tgctccagca actgagcgca caagggcgat aggtgatcgc atgtgtaagc agggtatgat
     3601 agtcgaattg gtcggcaaga agaaagatgc ctacacgcat cccgtagtcc agtgggccaa
     3661 ggagcaacgg gggcgaatcc tctgtccgaa ttgcggcaag ctcaggcaag acctcttccc
     3721 acagcccgtt gatgtgccgc tcatacagct ctacaaaggg acttcctaca gcattgtgtt
     3781 ccgcgggggg gtggccgtga ttcaccaacg tcttttggac ttcctgcgtc cacacttacc
     3841 cgaacatagc ctaggtcgtt gtttctggag agatggttcg cttctcccag actaccattc
     3901 gatctacttc cgggattaca tcttctttcg ggctggtaga gaaagcccac ggtatgagta
     3961 ctatgaatgc gagatctgcg gcttgtcggg agcaagctca gacgagccct acgttcttcg
     4021 cagtgagctg ccggatgcga ccgtgttcca ggacgcgatt cattgtcttt atctgagtgc
     4081 atccctggct cgcgtgttcc cctggcgaga attccgagat ctggagccct ttacgtttcc
     4141 agttcgcgat gagccgttgc ctgacgatcc tttgcctgtt gggtggcgac cggcgttcaa
     4201 aggccaggca tggctcaccg cgagaacgaa ctcgttgccg gagtccgttc gcgagcccga
     4261 gcctgaccgc tacgcagagc tgaaggcgat gacgctggac ttacctcggt tggaggatga
     4321 ttcggccaaa tcatgccccg tgccgatgcg gaagatcatg agttacctac ggctggaggg
     4381 agtcgaccga cggatcaagg ccagggactt gcagttcctg cgaaccgccc gcgtcgggga
     4441 ccgcgactgc tggatctggc ggtttgagga gcaagtgtgt gccgatgcat acgtgaccgt
     4501 ctggcgaggt ccagatggtg ccctttgcgt cggctatggc ggaaacagcc atggcctgac
     4561 gccggagcaa tacatctacg gcgaacatca caagtacttc tgatcgcctc ggctgacttc
     4621 ggaaccaacg acgccaacaa cacctacgtc agcggctccg agccgctgag cacggccgta
     4681 cccggcggca gctactggac cagcctgccg gccacgctcg acacaaccgc ctccccccgc
     4741 cccgacaact ccgtcggcca cctcttcccc ggtcaggagg ccgagaccgt cgcccggatg
     4801 ccacagatcc tgggcaactc catcttcgtc ctgcgcccca cgggtacaaa tccgtgagcc
     4861 cggtcttcgg cggcgcgcta gcggttttga tagcagtcag ggcacgatat cctgcgagcc
     4921 tgtgcggctg ggtgcgacca gacgggcaaa acctccagac cggagtcttc cctaagtctt
     4981 tgagcgttgc gggtttatgc aaacacaaac ggccccgcgc ggggccgtgc gaaatgcgtc
     5041 ctcaaggact cgaaccttga accctctgat taagagtcaa ggggatagct ctgtaaccaa
     5101 cgaaacgaga aaggtttatg ggcgtcccgg gcgcgcgcta gcgcttttga tagcagttga
     5161 cctctccaaa cttctgaact actacccgaa gagctatgct tatcagtctc caccgggttc
     5221 gtggtctcgc cgggaagggc ttcgttctgg tcgtcttgtt gtcatctgag gccatggccc
     5281 ggggccttgt tgcagcgatc atcttggtgt caccggaacc aaaacccctg cttacgacag
     5341 cgccagcggg ggtttcggtg acggaggcgg acccaatgag tcgtaggacc tcggctgaga
     5401 acgcgacctt ggaatctctg gatgtgtccc actgtctgca gataagcaga tgtgccgacg
     5461 acatcgcaga tggctatggc tgaccgggcc aactgcttaa catcatccgc ctctctccgc
     5521 tgatcgccat gaggccggga accagaccat ggtaacctga tccccgaggg ccagcgcgac
     5581 gctacccagg caagactggc cggggcgatg cgccgtgggg gcatgtcgca gagggagatc
     5641 ctcgccgcgc tgcaggtcac tcatcaagcc cgctgccagc ccccgctgcc ggatgacgag
     5701 gtggaaagga tcgcggccag cgtggcgcgt tacgcccccg acgcggtgtc ggtcgcgctg
     5761 atcgaggatc attacgccca gatcttcgga accgggcatg acacgaagcc ggtcggcgaa
     5821 tcatcgccgg ctgtcgaccc tggccctctg ccggacgagc tgttccgcgt gccggggttc
     5881 atcggcgagg taatggactt ctgcttggag accgcaccct acccgaacct ggcgctggcc
     5941 ttcggcggcg cgctggcgct tcaggcgttc ctggccggcc gcaaggtgcg cgatgccgcc
     6001 gacaaccgga ccaacctcta cgtcctggcc ctggccaaca gcggcgcggg caagaatgag
     6061 ccgcgcaaag tcaaccagcg catctgcgtc gaggtcggac ttcaggactg cctgggcgac
     6121 gcattcgcca gcggcgaggg catcgaggac cgactgttcg tcacgccgtc ggtcctgttc
     6181 cagaccgacg agattgacgg cctgatgaac gccatcaaca aggcctccga cgccaggcac
     6241 gagggcatca tgaacgtgct gctgaagatg tacacctcca gcagcaccat ccaccccatg
     6301 cgggtcaagg ccggcggcca gtcgccaggc gtcatcgacc agccgtcgct gtgcatgctc
     6361 ggcacggccg tgcccaagta ctactacgag gccctcaacg tccgcatgct caacaacggc
     6421 ttcttcgccc ggctcatcat cctggaggcg ggcaagcgcg gccggggcca gacgcccgtc
     6481 gcgcggccca tcccgcagtc catcctcgac acggcccgct ggtgggcgga ctaccgaccg
     6541 gcgacgggca acctccagga ctggcacccg atacccgtct gcgtcgaggc gacgccggag
     6601 gccgacgcgg cgatggagga gtgtcgcgcc tacgccgacg accgctacgc cgaggccgag
     6661 gagaaggacg acccggcggc catggctatc tgggcgcggg cctgtgagaa ggcccgcaag
     6721 ctcgcgctcg tccacgccgt cagcgccgac cacctccaac cccgcatcga cgaacgtgcc
     6781 gtccagtggg cctggcggtt cgtggactac cagacccgcc ggatgctctt tatggccggc
     6841 cagcacgtag ccgagggcga gttcgacgcc aagtgcaagc ggatgctgga ggtgctcacc
     6901 tcgtggcggc agcgacgcgg ggacgcctgg atgccgcatt gggaactgtc gcgccgcctc
     6961 aagtggagcg acaaggacat cgaggaggtc cgcaatgctc tgcgcggcca gcggcgcatc
     7021 gagcacgacg tgggctcgac cgccggaggc ggcccggtcg cccagcgata ccggctgctt
     7081 tgtcccggcg ggcagttgcc gcagttgcca cacgtaaatg tggcaactgg cggcgcgaac
     7141 gagtcggttg ccgaccagac cgcagggtcg ccgcacgagc cgatgcggga actggcggga
     7201 acgtaaggta tgaaaagaga agatattaca tcatcttctt ctcagttacc acattaccgc
     7261 atagtacccc cgcccgcgcg acgtgcgccc gcgcgtacgc gcgcgagggg tgcggtggcg
     7321 gcaaccggtg acttatgcgc aacagcctat cgcttccaca tccgcgccga ccggcgcgcg
     7381 aggtatccca tcagctttcc cttgcggact ttaggcaaag taacgcagcg cctcggatgc
     7441 cccggcaacg agcaccttag tgtcgccgca acaatgcacg cgccaggcgg gttggcgtac
     7501 cacctgcaac ccatgtttgc atccgagcaa cggcgcgaac ctgccccagt ttggcgatgg
     7561 ttcaacgtcg gcgtgcttca cctcgaccgg caaccagggc ttgccgtcgc ggacgataag
     7621 gaagtcgatc tcccgccgct ccttgtcgcg caagtagaac aggtcgaact cgccctcgcc
     7681 ggtgtccgtc cagtagtggc aggccttgag cagatgcgaa gcgacgaggt tctcgaaccg
     7741 ggccggctcg gtcggcaccg ccgcccaatc ccacaggtag acctttcctt cccggcgaag
     7801 cgaccgtgga atccggcggc tccaaggttt gacctcgaag aggtaataca cctccttcag
     7861 gtacatcagc catcggcgga ccgtgtcgaa gctcacttcc atggtttccc gcaggcttgc
     7921 cacgctgagc agcgagccga ctcgctccgg gagaagagca gcgagcagct cgatcctgcc
     7981 aaggtcgggc aggcggctga tgtcccggag gtcttcgcgg atgacgatct gctggtgatt
     8041 gcgcctccac agcctggccg ctcgctcgtc atgctcgaag agcggttcgg ggaacggccc
     8101 gtaggtcatc agggcgggca ggcggtcctc ggcgctgcgg ccaggcttga acgcgcggcc
     8161 gaacaacgcc tccagcgccc catccggcga caggaacgtt acgtcctcca tttcgcgcag
     8221 gctgaagggg tgcagacgaa agtggaaata gcgccccagc aggctgtcgc ttccgcgtcg
     8281 atagatgttg agcctggcgc tgccggtgac gagaaaatcg caggggcgtt ccaatgtgtc
     8341 gtacagccct ttgaggttgc gcttccaaag ccggtccttg tggatctcgt ccagcacaac
     8401 gaggggaacc gccctgccct tgccgggcgg aaggacaacg gagggttgct tggcccacgc
     8461 gtggcggaac tggaactcgt cccagttgtg atatccccca acggcgcgct cggccaggag
     8521 catcttggcc atcgtcgtct tgccgcactg gcgcggcccg gacaccagcg cgatcttgtg
     8581 gcgacgggcg aagcacaacc gtttgacggc ctcggccaga tagcggcttc tcatgccgac
     8641 catactacgg acagaccgca gtatggtcaa gagaaaaacc ggtctgacca aactcgtggc
     8701 aaacgcgagt ttggtcaggc cagccgcacg gttcctccgc cgcaagcgtg cgccgagacg
     8761 gtcgcgggaa cagccgccaa gcgcgacaga gtttgttttg cctgtccggc cggggcatgg
     8821 gccggtcgtg gcggcgtgtg ccctggcgtg gcggccccat gtcggcacgt tggccggcga
     8881 gacggaccac gccgtcgccg ggagccgccc caggacgcga tacgggccgc cctgggcgac
     8941 gcggcgctgc cgggtggtac cgtcagtacc cttgggaagc ctcgttgcaa cgtgaggcgt
     9001 ttctggcggt ctggcgggca atttgcacgg gattgcgctt attgcacggg attgcgttta
     9061 ttgcaagtgt tgcatggtga caaggaggtc acgagatgaa cattgagttg cggcctttgg
     9121 cccagatcaa gccttacgag aacaatcccc ggatcaacga tgcggcggtc gacgcggtag
     9181 cggaatccat ccgccggttc ggctttcgcc agccgatcgt ggtcgatgcc gacggcgtca
     9241 tcgtctgcgg ccacacccgc tggaaggcgg cccagaagct cgggctggag caggtgccgg
     9301 tccacgtggc caaggacctg acgcccgagc agatccgagc gtaccgcatc gccgacaaca
     9361 agaccgcgga actggccgag tggaacctgg agctgctgcc gatcgagctg gccgagttgc
     9421 agggtgccgg catcgactgg tcgctgctcg gcttcgatgg tgatgagttg gccaagctgc
     9481 tcgatccggg cgtcaagcag ggtttgaccg accccgacga ggtccccgag ccgccggatg
     9541 aggccatcac ccagccgggg gacatctggg tgctcggcga ccatcgtctg atgtgcggcg
     9601 acgcggcccg ggccgaggac gtggaccggc tgctggacgg ccggccgatc cacctggtca
     9661 acaccgaccc gccgtacaac gtgaaagtcg agccgcggtc caacaacgcg attgccgctg
     9721 ggctgtcatc gttcggcgag gcgggcgtga cgaaccacca gggcttcgac ctggcccgtc
     9781 ggccggacgt ggcccggccg acgcatcgca agctgcggcc caaggatcgc ctgctggcca
     9841 acgacttcgt cagcgacgag gagttcgagc ggctgttgga cgcttggttc tcccaactgg
     9901 cccgcgtgct cgagcccggg cgggccttct acatctgggg cggctacgcc aactgcggca
     9961 actacccacc gttcctcaag aagcatggtc tgtacttctc gcaagcgatc atctgggaca
    10021 agcagcatcc agtcctgacc cggaaggact acatgggcgc ccatgagtgg tgtttctacg
    10081 gttggaggga aggggcggct catgtcttcc tcggtccgaa caacgctacg gacctctggc
    10141 atgtcaagaa gatcaacccg cagagcatga tccatttgac cgagaagccg gtcgagctgg
    10201 cggtgcgggc catgcagtac tcctcccggg tcggcgagaa cgtgctggac ttgttcgggg
    10261 gcagcggttc gacgctgatt gccgccgaac agaccgcacg tcgggcgtac ctgatggaac
    10321 tggacccgct gtactgcgat gtcatcgtcg agaggtggtc caaattcacc gggcggaacg
    10381 cggagaggat ttcaggccag agcaacgccc cggtcgaggc cgaggcgttg gaggtggagg
    10441 cgtcatgcgc cagcgatcag gcgtgatggt cgttgtactc gaactggccg cgatcggtct
    10501 tgcggaatcg ggaaacggtg ccgttttcat ccgccttctt gatctcgcgg ctcatggcag
    10561 cgtacaacgt ggcctcgggc gtcttgccgc cggggctgga ccacaggccc tgggcggcca
    10621 tggcctcgat cagttccttg gcccgcatgg gcttgccttc ggtcttgagc acctcggcgg
    10681 cggcgtccag ggcgctgagc cgcttgggct ggtcggcggc ggcctttttg gccttagcag
    10741 cgcggacctt cgcgagccgc tcggcctcct cgagcgtcag ctcactgcgt ggggccttgt
    10801 ccaggtcgcg gtcgtcgcgc ttttgagcct cggactgctt attcgtggac ttgctctttg
    10861 gcatggcctt cacctgttca ggggtctgtg gttctccgat cgggtgacac gcctctgcct
    10921 gtgcgtcgca cgcagacggg tcgatcgtgt tctcgccgac ttcgctgggc tcctgctcct
    10981 cgcagtgcct ctcccagcac gcctggcaca gcgggcgatc gagatatgtc atgacgggcg
    11041 tgcccttgca cccttccgtg gcgcagcgcg ccgggtcata gcccggcctg tcctcctctg
    11101 cttcggactg agctgcctcg gcgggcacga tgctcggtgg tgcggtggtt tcaacaacgg
    11161 ctgcatttgc agcaggcggc ttaccgccgt tcttcggcga aaccttatgc cgcagccgct
    11221 ggggactctt gatacggatg cgtttgcccg tggcggtgtt cgtcgcgttc cagccgccgt
    11281 ggctgttga
//
LOCUS       DAOTPW010000183        11265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5448_length_11265_cov_4.206155, whole genome shotgun sequence.
ACCESSION   DAOTPW010000183 DAOTPW010000000
VERSION     DAOTPW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5448_length_11265_cov_4.206155"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   31..1912
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=31..66
                     /rpt_unit_seq="agtgtaatcgaccccgacctgcgagcaagccgcaac"
     gene            complement(2295..2840)
                     /locus_tag="P5316_15510"
     CDS             complement(2295..2840)
                     /locus_tag="P5316_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRR86415.1"
                     /translation="MLKEQPNRSPTRGARSWLDLSDSQLLAQCEVDCYRASGPGGQKR
                     NKTSSAVRLRHGPTSLIVTAAESRSQHENKARALRRLREAIAMTQRNPLRPGDPPPDF
                     FLSALSRDPGLRVNRKNPDYYHIVQYVLDMFSACAGSTADTARSLGISAGHLIRFLKN
                     DDTLWEHANRLRRKFGHATLR"
     gene            complement(3056..4825)
                     /locus_tag="P5316_15515"
     CDS             complement(3056..4825)
                     /locus_tag="P5316_15515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRR86416.1"
                     /translation="MNDELTVTAGSDRGSLAHTQARPCEDRAGLIRAAFITLALAQIV
                     LLSVLGLGCTVGPDYRRPDLPMPAEWSSLQADAAATQPASAPASPKPFAERHPDPLWQ
                     AQGPTTRPTSRPCDIGSWWRKLNDPTLDSLIDRAVQSNLDLKIATARVREARAQRNSV
                     AAGLWPQIGAAGSYNYGGGSLNAGREASGGTGLGKQVRNTAVNSAVQSLVNGQGIDPA
                     QIASNAAKQALSTAVNNKLSDEGRVSHRGQNVFQAGFDASWELDVFGGVRRGLEAADA
                     EVVASVEDQRAVIVSLVAEVALEYVQLRGYQRRLAIAHKNIEAQRKTVELTQDRRSVG
                     FTNDLEVAQARTQLATTTSQVPVLQDSVRQTMYRLSILLGLPPGALVPELEEASAIPT
                     SPPEVPIGLPSELLRRRPDVSSAERQLAAATARIGEAIADLFPKFSITGSFGTQSRDI
                     RYMLDRDSLVWSLGPAVSWPIFQGGRIRANIEIQNARQEQALAAYEQTVLNALNEVEA
                     ALSAYLNEQIRRQALLEAVETSRQATELSTRLYVRGLGAFLNVLEAQRSLYATEEALV
                     QSDTAIITDLIALYKALGGGWEG"
     gene            complement(4836..6209)
                     /locus_tag="P5316_15520"
     CDS             complement(4836..6209)
                     /locus_tag="P5316_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR86417.1"
                     /translation="MRISRTLRTAMRALRRNPMRATLTTLGIVIGVGAVIAMMEIGAG
                     SSAQIQKGISSMGANVLLIMPGTVSTAGVTQSAGSVITLTPQDCEAIIRQCPAVRTAA
                     PVVRARTQVVCGSRNWEPNSIYGTTPDFLEVRQLGPMVEGRMFTQRDILNGNRVCVVG
                     KTLVRELFRGASPVGREIRIKNVTFRVVGVLRSKGANMMGMDQDDIILAPWSTIKYRV
                     AGSSLGSTNQSISSGSSSSAVNTLSNLYPGGQTSLYPQRSEVQMADNPMPVRFTNVDQ
                     ILAVARSPQSIPAAMQQIKEVLRRRHRLGPDDPDDFDLRDMTEMSNVLTSTTSMMTNL
                     LLAVATISLIVGGVGIMNIMLVSVTERTREIGLRMAVGAQPRDILRQFLVEAVVLCLI
                     GGAIGISLGRAASICVRMLLNWPTALSVGAIIAAVVVSAAVGILFGYYPAWKASRLDP
                     IEALRYE"
     gene            complement(6206..6949)
                     /locus_tag="P5316_15525"
     CDS             complement(6206..6949)
                     /locus_tag="P5316_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR86418.1"
                     /translation="MIELQNICKTYHIGEISVPALKNVSLSVERGELLALMGASGSGK
                     STLMNILGCLDRPSSGRYWLDGRDVSKLSIAERAMERNRKIGFVFQNFNLLPRTSALD
                     NVAMPLSYAAEHLSEREARRRARQILCRLGLEDRLHHEPSQLSGGQQQRVAIARALVN
                     RPSILFADEPTGNLDSQTSVDVLRIFQQLNEGDGLTVILVTHDANVARFARRIVHIRD
                     GTISNGLFTGDGGAKSGSMAGYVENGAGI"
     gene            complement(6961..8316)
                     /locus_tag="P5316_15530"
     CDS             complement(6961..8316)
                     /locus_tag="P5316_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR86419.1"
                     /translation="MKKILVLIILTGLLVGGGAVYWHRGSTSAAAFRTAQVQRGDMII
                     SIAATGTVEPEEVIDVGAQVAGQILSFGTDANGRTVDYGSPVEADTVLARIDDALYTS
                     EVEQAKAQVLSAEGGLKRAEADLEQAKAKLYQAERNWERARQLGSSSSALAATDYDNY
                     KASYDSARASVLICEAAIVEAKGNLAQARSMLQRAERNLKYTTIKSPVKGVIIDRRVN
                     IGQTVISSMNAPSLFLIAKDLTRIQVWVAVNEADIGRLRPGQPVTFTVSAFPGETFKG
                     EVGKIRLNASMTQNVVTYTVEVVADNPNGRLLPYLTADVKFQLDRHNGVLMVPTDALR
                     WTPTADQVLPEVRRTLENQSAAGPRHAVAKETAPSFTDTDVQEQGILWVPEGRHVRPV
                     FVRLGLSDEDMTEVEGDGLVEGMAVVTGQEEIQETSAGGSNPFLPKFPRMGKRRPPPP
                     M"
     gene            8603..8824
                     /locus_tag="P5316_15535"
     CDS             8603..8824
                     /locus_tag="P5316_15535"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRR86420.1"
                     /translation="MDEKVTYWVDLAGYDMDTAKAMLQTGRLLYVGFMCHQVVEKMLK
                     AWWVSAKGSTPPYTQPRGARSAEWSVRPP"
     gene            8966..9277
                     /locus_tag="P5316_15540"
     CDS             8966..9277
                     /locus_tag="P5316_15540"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRR86421.1"
                     /translation="MDTQQAIKLVKRYADVVRAQFPVRKVVLYGSHARGTAHEYSDID
                     VAVLVDRIEGDLLDLEATLFRLGRGVDFRIEPVLREEGHDPSGLTAEILRTGHVVYSA
                     A"
     gene            complement(9470..11047)
                     /locus_tag="P5316_15545"
     CDS             complement(9470..11047)
                     /locus_tag="P5316_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR86422.1"
                     /translation="MVNLFHRNWWAFCTQVVGSPCVWVGARRKAVRLRSIQWTLQLWY
                     AGVLAVVLVGFGSASYFGISRARWEQLDSELARSVQELAAGFRPPKMPEPSSVPASTT
                     NAGDSVSRDPWQDLPPPDFELPAALTKRFEPNEPESDYYVIWDREGRVLASSNVRFAA
                     GVPYPGVRPHLSVRPHPPGPPQIRKRGEFREAYINGPRWTRILVGTSVRPTEIELRHF
                     AFSLTAIGAGVLAAGLVGGWLVSLRAVRPIRAITEVAQEISASNLSRRIPVAEVQSEL
                     GSLAVVLNEMIDRLEAAFQQQVRFTADASHELRTPLSVIHTHVQLALSRERTADEYRR
                     TIETCRRASSRMKDLVDSLLLLAGADAGRLTLNRQLADLRGVVENCTAMVAPLAEAKD
                     VVIETDLHPAELSVDVSRITQVTTNLLTNAIRYNRKGGRVEIRLDVDGPVVVLAVSDT
                     GLGIPLENQPHIFERFYRVDKARSREEGGSGLGLAICKTIVEAHGGTISFRSKPGQGT
                     TFEVRLPLIETRMAKSL"
     gene            complement(11040..>11265)
                     /locus_tag="P5316_15550"
     CDS             complement(11040..>11265)
                     /locus_tag="P5316_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRR86423.1"
                     /translation="VATAVDFGTMIALVELLALHRGKLVTRTMIYEHLFDESNDTLSN
                     LVDVHVANVRKKLGKEFIITRRGEGYQVHG"
BASE COUNT         2130 a         3460 c         3545 g         2130 t
ORIGIN      
        1 taggcattgg ggcggcggat aaagacgctg agtgtaatcg accccgacct gcgagcaagc
       61 cgcaacgcgc caattgatcc aggccacccg gcctacagtg taatcgaccc cgacctgcga
      121 gcaagccgca acgaccgact acaaggggct gcccggcatt gtagtgtaat cgaccccgac
      181 ctgcgagcaa gccgcaacgt gcatgagcat atcaccaccc cacccacaag tgtaatcgac
      241 cccgacctgc gagcaagccg caacggtggc tccatgcttg aggcgaggcg gtccagtgta
      301 atcgaccccg acctgcgagc aagccgcaac ctccaacggt gaggatgtct ttttgcttcg
      361 agtgtaatcg accccgacct gcgagcaagc cgcaaccaaa agtagaactt agtagcggta
      421 ctgagcagtg taatcgaccc cgacctgcga gcaagccgca acgcccggcg gacctgctgc
      481 acgtgcgcgg tagtgtaatc gaccccgacc tgcgagcaag ccgcaacctg cgattccggc
      541 ctgcctcact gcatgatagt gtaatcgacc ccgacctgcg agcaagccgc aacgatgacc
      601 ctcggatggc cagcgccgat catagtgtaa tcgaccccga cctgcgagca agccgcaact
      661 tgcaggagtg ggaaacgctg gccccgccca gtgtaatcga ccccgacctg cgagcaagcc
      721 gcaaccgtca cgttcgtggc ggccagcaat cgccgagtgt aatcgacccc gacctgcgag
      781 caagccgcaa cgggaggccg tctgggcgca gcagagtcgc gagtgtaatc gaccccgacc
      841 tgcgagcaag ccgcaaccgc gcaagtcatt ccctgtgagt aaaaccagtg taatcgaccc
      901 cgacctgcga gcaagccgca acgcgtctct gatcagggcc cgactgcctg ttagtgtaat
      961 cgaccccgac ctgcgagcaa gccgcaacgc tgatctagta ggcgccaatc tggcccgcag
     1021 tgtaatcgac cccgacctgc gagcaagccg caacgcctct ctgatccagg cccgactgcc
     1081 tgttagtgta atcgaccccg acctgcgagc aagccgcaac cgcaatggag tctctgctat
     1141 gaaaccgagt agtgtaatcg accccgacct gcgagcaagc cgcaactgat atcgcccaga
     1201 caccggaatt cgttcgagtg taatcgaccc cgacctgcga gcaagccgca acatatctgg
     1261 ccgggtgtgc cggccgagtc tagtgtaatc gaccccgacc tgcgagcaag ccgcaacgct
     1321 atgccggacg attgccggct gaattctagt gtaatcgacc ccgacctgcg agcaagccgc
     1381 aacgccacga gcgcaggata ttcatccacg aatagtgtaa tcgaccccga cctgcgagca
     1441 agccgcaact tagcgaccgt gtagggccga ccagaatgca agtgtaatcg accccgacct
     1501 gcgagcaagc cgcaactacc gaggcaatcg aagcagcacg ctggcgagtg taatcgaccc
     1561 cgacctgcga gcaagccgca acccggcccg gattgcggtc agccagaccg cgagtgtaat
     1621 cgaccccgac ctgcgagcaa gccgcaactc gatgggctac gatgatcagc agcggtagag
     1681 tgtaatcgac cccgacctgc gagcaagccg caactctcaa tgtcgaatgc tagcctttgc
     1741 acgtagtgta atcgaccccg acctgcgagc aagccgcaac tgcggcggca atgttttgtg
     1801 gttgttattt agtgtaatcg accccgacct gcgagcaagc cgcaacggaa gcgtcagcgc
     1861 cagaatagag gcgactagtg tgatcgcccc cagcctggga gcattccgct cacggcggat
     1921 catgggtggt ttgggatctc atttttgcgt ccccgggttt gggaaaaacc ggggaccttt
     1981 ttttgtcgaa tcgccatcat ccgaaagccc ccggaccctc tagcccccaa cgtccggcac
     2041 gcctttttat gtcgtgttgt gttcaccgtg tcgaatccgc cgggacggac tcttggtggg
     2101 cgtctatccc gaccacttgc gatccagcct tctccgcgtt ttccacggcg tagcgcaacg
     2161 aagacggcgc ggcttgttac cttgccccgc gtctccggcc accacgatcc gtgtgatccg
     2221 cgtggtctgc gggcccagct ttctgcttct ttacccgcaa ccggaccgtc tggttcgcgg
     2281 gcacccatcc gtcgtcagcg cagtgtcgcg tgtccgaact tccggcgaag ccggttggcg
     2341 tgttcccaca acgtgtcgtc gttcttcagg aagcgaatca ggtggcctgc tgaaatgccc
     2401 agcgagcggg cggtgtcggc tgtgctgcct gcgcaggcgg agaacatatc caggacatat
     2461 tgaacaatgt ggtagtagtc ggggtttttt ctgttcaccc tgagaccggg atctcgggac
     2521 aacgcggaaa ggaagaagtc cggcggcgga tcgcccggtc tgagggggtt tcgctgcgtc
     2581 atggcgatgg cttcgcgaag acgcctgagt gcacgggcct tgttctcgtg ttgagagcgg
     2641 ctctcggcag ccgtcacaat gagcgaggtg gggccatggc ggagtcggac ggccgaggag
     2701 gtcttgttgc gtttctgccc cccggggccg ctggcgcggt agcaatcgac ttcgcactga
     2761 gccagcaact gggaatccga gagatcgagc caggatcgcg ctcccctggt cggcgaccgg
     2821 tttggctgtt ccttcagcat gatgaccgtt tccgatgctc gtgaatcccg gccgtccgcc
     2881 ggccggttac cgcccctggc cgtctgccct gtctgaagcc tcgtcggcag aagggatcag
     2941 ccgctctgtt tgccagcttc gggcggcacc gacacggcgc aacacatgat actcgaacga
     3001 ggtgtcggaa aaagcctcac gagcgcgaga gtgcaatcgg tcgttttcgg atctctcagc
     3061 cctcccatcc tccgccgagc gccttgtaca gggcgatcag gtcggtgatg atcgcggtgt
     3121 cgctttggac cagagcctcc tcggtggcgt acagtgagcg ttgtgcctca agcacgttca
     3181 ggaacgctcc cagtccgcgg acatagagtc tcgtgctcaa ctccgtcgcc tggcggcttg
     3241 tttcgacggc ctcgagcaat gcctggcggc ggatttgttc attcaagtag gcggagagtg
     3301 cggcctcgac ctcgttgagc gcgttgagca cggtttgttc gtatgctgcc agagcctgct
     3361 cctgccgggc gttctgtatc tcgatgttgg cccggatgcg tccgccctga aagatgggcc
     3421 atgagaccgc gggtccgagc gaccagacga ggctatcgcg gtcgagcata tatcggatgt
     3481 cgcggctctg ggttccgaag gagccggtga tggagaactt gggaaacaag tcggctattg
     3541 cctcgccgat gcgagcggtg gccgccgcca gttgtcgctc ggctgaagac acgtccggcc
     3601 ggcggcgcag gagttcggag ggcaggccta tgggcacctc aggcgggctt gtgggaatcg
     3661 ccgaggcttc ttcgagttcc ggcaccaacg caccgggcgg cagtccaagc aggatgctca
     3721 ggcgatacat ggtctgtcga acggagtcct gaaggacagg cacctgcgat gtggtcgtgg
     3781 caagttgcgt cctggcctgc gcaacctcca agtcattcgt gaagcccact gagcgtctgt
     3841 cctgggtcag ctcaacggtt ttccgttgtg cctcgatgtt cttgtgggcg atggccaacc
     3901 gccgctggta gccccgcaat tggacgtatt cgagggccac ctcggccacc agcgagacga
     3961 tcaccgctcg ttgatcctca acggaagcga cgacttctgc gtcggccgcc tcgagtccgc
     4021 gccgcacgcc tccaaagaca tcaagctccc agcttgcatc gaacccggcc tgaaagacgt
     4081 tctggccgcg atgcgagacc cggccctcgt ccgagagttt gttattgacg gcggttgaga
     4141 gcgcctgctt ggccgcattg gaagcgattt gagcggggtc gatgccctga ccgttgacca
     4201 gggattggac ggccgaattg acggcggtat tcctcacctg tttgcccaat ccggttccgc
     4261 cggaggcctc gcggccggcg ttcaggctgc ctccgccgta gttgtagctg ccggcggcgc
     4321 cgatctgggg ccagagtccg gcggctacgg agtttcgttg cgctcttgcc tcgcggacac
     4381 gagcggtggc gattttcaag tccaggttcg actggacggc ccggtcgatc agcgaatcga
     4441 gcgttggatc attcagcttc ctccaccagc tcccgatgtc gcacggccgg ctcgtcggcc
     4501 gagtcgtcgg tccttgggct tgccaaagcg ggtctgggtg ccgttcggca aacggcttgg
     4561 ggcttgcagg ggcactggcg ggctgcgtcg ctgccgcgtc cgcttgcagg ctggaccatt
     4621 ccgccggcat gggcagatcc gggcgccggt agtccggtcc gaccgtgcag cccaaaccaa
     4681 ggacggatag cagcacaatc tgtgcgagag ccagggtaat gaaagcggcc cggatcaaac
     4741 cggcgcgatc ttcgcatggt ctggcctgcg tatgagccag ggatcctcga tccgatccgg
     4801 ccgtaaccgt caattcatcg ttcatcctgt attcctcatt cgtaccgcag cgcctcgatc
     4861 ggatcgagcc gggaggcttt ccaggccgga tagtagccga acaagatgcc cacggccgcg
     4921 gagaccacca cggcggcgat gatggctccc acagacagcg cggtcggcca gttgagcagc
     4981 atgcggacgc agatcgaggc cgcacgtccc agagagattc cgattgcacc gccgatcaag
     5041 cacagaacga ccgcttcgac caggaactgg cgcaggatgt cgcgcggctg ggcgccgacg
     5101 gccatgcgca gcccgatctc acgggtgcgt tcggtaaccg agaccagcat gatattcatg
     5161 atgccgacgc caccgacgat gagtgagatc gtcgccacgg caagaagcag gttggtcatc
     5221 atggacgttg ttgacgtcag aacgttgctc atctccgtca tgtcgcggag gtcaaagtcg
     5281 tccggatcgt cagggccgag gcgatgacgc cgccgcagga cctccttgat ttgctgcatt
     5341 gcggcgggaa tcgattgagg ggagcgggcg acggccagaa tctggtccac gttggtgaac
     5401 cgtaccggca tggggttgtc ggccatctgg acctcgctgc gttgcggata caggctggtc
     5461 tgaccgccag ggtagagatt gctcagagtg ttgactgcac tgctgctcga gccgctgctg
     5521 atactctggt tggttgatcc cagcgacgag ccggcgaccc ggtacttaat cgtggaccaa
     5581 ggggcgagga tgatgtcatc ctgatccatt cccatcatgt tggccccttt gctcctaagg
     5641 accccgacga cccgaaaggt cacattcttg attcggattt ccctgccgac aggcgaggcc
     5701 cctcgaaaca gctcgcgaac cagggttttg ccgacgacgc agacccggtt tccgttgagg
     5761 atgtcgcgtt gggtgaacat cctgccttcg accatggggc ccagttgccg gacttcgagg
     5821 aaatccgggg tcgtcccata gatggagttg ggctcccagt tgcggctgcc gcagacgacc
     5881 tgagttctgg cccgcacgac gggggcggcc gtgcggaccg cggggcactg gcgaataatg
     5941 gcctcgcagt cctgcggggt cagggtgatc acgctgccgg cgctttgagt cacgccggcc
     6001 gtggaaaccg tgccgggcat gatgagcagg acgttggcgc ccatgctgga gatgcccttt
     6061 tgaatctgcg cagaggaacc cgcgccgatt tccatcatgg cgatgaccgc cccgacaccg
     6121 atgacgattc ccagcgtggt cagcgtggcc cgcatgggat tgcgccgcag agccctcatg
     6181 gcggttcgaa gggttcgcga gattctcata taccggctcc gttttccacg taccccgcca
     6241 ttgaacctga tttcgctccg ccatcgccgg taaagagccc gtttgagatg gtgccgtcgc
     6301 ggatgtggac gatccgtcgc gcgaacctgg cgacgttggc atcgtgggtg accaggatga
     6361 cggtcagacc gtcgccttcg ttgagctgct gaaagatgcg aagaacgtcc acgctggtct
     6421 gggaatcgag gttgcccgtg ggctcgtcgg caaagaggat tgagggacgg ttaaccagag
     6481 cacgggcgat ggcaactcgc tgttgctggc cgccggacaa ctgggagggc tcgtggtgca
     6541 gccggtcttc gagcccgagc cggcagagta tttggcgggc gcggcgacgg gcttcgcgtt
     6601 cggacaggtg ctcggcggcg tacgacaggg gcatggccac gttatcgagg gcgctggtgc
     6661 gcggcagcag gttgaagttc tgaaacacga agccgatttt ccgatttctc tccattgccc
     6721 gctcggcgat cgacaacttg gaaacgtccc tgccatcaag ccagtatcga ccggaactcg
     6781 gccgatcgag gcatccgaga atgttcatca acgtgctctt gcccgagccg gaggcaccca
     6841 tgagggcgag gagctctccc cgctcgacgc tcagcgacac attcttcagc gcaggcacgc
     6901 tgatctcgcc gatgtggtag gtcttacaaa tgttctgaag ctcaatcatt ggcgcacgat
     6961 ctacatgggc ggcggcggcc tgcgtttacc catgcgaggg aacttaggca ggaacggatt
     7021 gctgccgccg gcggacgtct cctgaatttc ttcctgcccc gtcacgaccg ccattccttc
     7081 gaccagtccg tcaccttcga cctcggtcat gtcctcatcg ctcaagccga gccggacgaa
     7141 aacgggccgc acatgccgtc cctcgggtac ccagaggatg ccctgctcct ggacgtcggt
     7201 atctgtaaac gacggcgcgg tttctttcgc gacggcatgc cgaggcccgg cggccgactg
     7261 gttctccagg gtccggcgaa cctcgggcaa gacctggtcc gcggtcggcg tccatctcag
     7321 agcatcggtt ggaaccatca gcacgccgtt atgacgatcg agttggaact tgacgtcggc
     7381 ggtgaggtaa ggcaacagtc ttccgttggg attgtcggcc acgacttcca cggtgtaagt
     7441 cacgacattc tgggtcatgg acgcgttcag gcgaatcttg cccacctctc ccttgaaggt
     7501 ctcgcccggg aatgcggaaa cggtgaacgt cacgggttgg ccggggcgca aacgaccgat
     7561 gtcggcctca ttcacggcca cccatacctg tatgcgcgtc aggtctttgg cgatcaggaa
     7621 aaggctgggg gcgttcatgc tggaaataac ggtttgacct atattcacgc ggcgatcgat
     7681 gatgacgcct ttaaccggag acttgatggt ggtgtacttc agattgcgtt ccgcgcgctg
     7741 cagcatgctc ctggcttgag caaggttgcc cttggcctca acgatcgccg cttcgcagat
     7801 gagcacgctt gccctggccg agtcgtagct tgccttgtag ttgtcgtaat cggtggctgc
     7861 cagcgccgat gatgacgagc ccaactgtcg tgctcgctcc cagtttcgct cggcctggta
     7921 gagcttggcc ttggcctgct ccaagtccgc ctcggcccgc ttgagcccgc cttccgcgga
     7981 caagacctgt gccttggctt gttcgacctc ggaggtgtag agggcgtcgt cgatcctggc
     8041 caataccgtg tcggcctcga ccggcgagcc gtagtccacc gtcctgccgt tggcgtcggt
     8101 gccaaacgag aggatctggc cggcgacctg ggcgccgacg tcgatgacct cttccggttc
     8161 aacggtaccg gtggcggcga tcgatatgat catgtcgccc cgttggacct gcgcggtgcg
     8221 aaaagcggcc gcggatgtgc tgccacgatg ccagtacacc gcaccgcccc cgacgagcag
     8281 acccgtgagt atgatgagca ccaggatttt cttcatggct agccccgtac cgtcgactgt
     8341 cgacgccgat cccgacggcg ttcagatcga cggccaccgg gattctatgg atgtcaacct
     8401 taaggcaaga tgaagacgcg ccggacgcac gctggaggtg atcgccccgc gcgtcctgca
     8461 gctcaagctg acgggatcga ggcagttcgc tcgctggccg ggcgataccc gaggccggac
     8521 gctggatgcc tggccccccc gcgtgtgatc cgagaaacgc tttgcagctt gtgagcattc
     8581 gttccggtcg tatgataaga tcgtggacga gaaggtgaca tactgggtcg acttggccgg
     8641 ctacgacatg gacactgcca aggccatgct ccagaccggc cggttgctgt atgtgggctt
     8701 catgtgccat caggtggtgg agaagatgct taaggcatgg tgggtgtcgg ccaagggttc
     8761 aacgccgccg tacacacagc ctcgtggcgc tcgcagcgct gagtggtctg ttcgacctcc
     8821 ttgatccgac acgacgcgcc ctgctggaca cgctggggcc gctgaatgtc gaaaactgct
     8881 acccggcgga ccggcagcgg attctggcat ctctgacacc tgagcgatgc gaggggctga
     8941 tcagccagac cgaggagttg cggcaatgga tacacagcag gctatcaaac tcgtgaagcg
     9001 atacgccgac gtggtccggg cgcagtttcc ggtgcgcaag gtcgtcctct atggatctca
     9061 cgcgcgcggc acggcccatg aatacagcga catcgacgtg gccgtgctgg tggaccgcat
     9121 cgaaggcgat ctgctggatc tcgaggcgac gctgttccgt ctgggccgag gggttgactt
     9181 ccgcatcgag ccggtgctgc gggaggaagg gcacgacccg agcggcctga ccgcggagat
     9241 cctgcgaacc ggacacgtgg tctactcggc cgcatgagtt ggctgacagg cgaacacggg
     9301 ccgtgggaca agacggccgg cgcgatgatc gctgcataga aaataacgaa taacgcagcg
     9361 agcacagtgg ccctccttgg ttattcggtc cgtcacggtg aatcttcaac attcgggttc
     9421 cgggtctgat tcgacattcg gccttcggct ctgaggctga gcctggggct caaagagatt
     9481 tcgccattcg ggtttcgata agaggcaatc ggacctcgaa cgttgtgccc tggcccggtt
     9541 tgctgcggaa tgagatcgtc ccgccgtgag cctcgacaat ggtcttacag atcgccagcc
     9601 ccagaccgct gccgccctcc tcgcggctgc gggctttatc gacgcgatag aagcgctcga
     9661 agatatgtgg ttggttctca agggggatcc caagacccgt atcactgaca gccaatacga
     9721 cgacagggcc gtcgacatcc aagcggatct cgacgcggcc gcccttgcgg ttataccgga
     9781 ttgcgttggt cagcaggttc gtggtgacct gcgtgatccg agacacatca accgacagtt
     9841 ccgcggggtg caaatcagtc tcaatgacga catccttggc ctccgccaag ggagcgacca
     9901 tggcggtgca gttttccacg accccccgca agtcggcgag ctgtcggttc aacgtgagcc
     9961 ggccggcgtc ggcgccggcc aggagcagga gcgagtccac gagatccttc atccggctcg
    10021 atgcccgccg gcaggtttcg atcgtcctgc ggtactcgtc agcggtccgt tcccgcgaaa
    10081 gcgccagttg aacgtgagta tggatgaccg acagcggcgt gcgaagctcg tgtgaggcat
    10141 cggcggtgaa gcggacctgt tgctgaaaag ccgcctcaag ccggtcaatc atctcgttga
    10201 gaacgactgc aaggctccca agttcgctct ggacttcggc gacagggata cgccgggaga
    10261 ggttggaggc agagatttcc tgcgccacct cggtgatggc gcgaatcggc ctgacggcgc
    10321 gaagcgagac cagccacccg ccaaccaagc cggcggccaa tacgcccgcg ccaatcgcgg
    10381 tgagcgagaa ggcaaaatgc cgcaactcga tttctgtcgg acgaacggat gtgccgacca
    10441 ggatccgagt ccatcgcggg ccgttgatgt aggcctcacg gaattcgcct cttttgcgga
    10501 tctgcggcgg gccgggcgga tggggtcgaa cagacaggtg gggacgcact ccaggatagg
    10561 gaacgccggc agcgaagcgg acgttcgacg acgccagaac gcggccctcg cgatcccaga
    10621 tgacgtagta gtcagattcg ggttcgttgg gctcaaaccg tttggtcaag gcggcgggca
    10681 gttcaaagtc cggcgggggc aggtcctgcc acgggtcgcg agagacgcta tctcccgcgt
    10741 tggtcgtcga tgccggcaca gatgacggct ccggcatttt cggcggccga aatccggcgg
    10801 ccagctcctg gacggaccgc gccagttccg agtccaactg ttcccaccga gctcgcgaga
    10861 ttccgaagta tgaggcggat ccaaagccga caagcacgac ggccagcacg cctgcgtacc
    10921 acagttgcag cgtccattgt atggacctga ggcggaccgc cttcctgcgg gccccaaccc
    10981 agacgcacgg tgacccaaca acctgcgtac aaaatgccca ccagttacga tgaaagagat
    11041 taaccatgca cttgatatcc ctcgccccgg cgggtgatga tgaactcctt cccaagtttc
    11101 tttcgcacgt tggcgacgtg cacatcgacg aggttggaca gggtgtcatt gctttcgtcg
    11161 aacaggtgtt cgtagatcat cgttcgcgtg accagcttgc cccggtgcaa cgccaggagc
    11221 tcgacgagcg cgatcatggt gccgaagtcg acggccgtcg cgacg
//
LOCUS       DAOTPW010000184        11169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5501_length_11169_cov_5.288825, whole genome shotgun sequence.
ACCESSION   DAOTPW010000184 DAOTPW010000000
VERSION     DAOTPW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5501_length_11169_cov_5.288825"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="P5316_15555"
     CDS             complement(<1..722)
                     /locus_tag="P5316_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR86424.1"
                     /translation="MPADRCDVGGVFVPLLEVLELTKEFPTPIGPPKRAVDHLSFHVN
                     AGEIYGLLGPNGAGKTTTLRIVSGLMRPTAGRAIVNGVDVTIDPSSVRGFIGFLTAGT
                     GLYHRLTAREILVYFAELNGLERGLITRRVDQLIDWLGMADFAELRCGALSTGQKQRT
                     NIARALIADPPVLVMDEPTLGLDVLTNRLILDFIRHERELGKAILLSTHHLDEAEALC
                     DRIGLLHEGRLIAEGNLQTLRE"
     gene            1052..1828
                     /locus_tag="P5316_15560"
     CDS             1052..1828
                     /locus_tag="P5316_15560"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRR86425.1"
                     /translation="MTTRRIIIVVAASLCLVGLSAVVPGFPQASLAAQVPAEPAASPA
                     SSGPVKTLLVTGGPIHDGKAIGDLVEETMKKSGLFDVTRAHEDLDAFLADRIAPFDLV
                     VFYYTLGELKEAQKRGLMSHIASGKGYVTFHSGADSFRGDPDYQAFVGGYFRTHPHYR
                     QYQVSITENDSPITKGIDEFMITDEQYILDYDPRVTVLANALFKGKLMPVMWTKPWGK
                     GRVFYLALGHDVKACQQEMFKKLLLRGSLWAAGRPVVDPK"
     gene            complement(1832..3784)
                     /locus_tag="P5316_15565"
     CDS             complement(1832..3784)
                     /locus_tag="P5316_15565"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRR86426.1"
                     /translation="MIVLAILGVSVVFLLLGGWIYAPIIGRVLGERADRVTPAVAIND
                     GRDYVPTRTPIVFAHHFASIAGAGPIIGPVLALLYGWGPALIWILLGGVFLGAVHDYV
                     VAHIAMREGGKNLTVIARRYVGPAAFLMMLVLLVALLVLVCAAFLDLSATALTSKVPV
                     ATLKMQPGETIFREVSWVNDMTGQEEPHAIIGGIASTSVVVITAFSPLIGFLYIKRRW
                     PVWLCSGLAILICAVSVAIGLYLPMAVEPMTWKLLISIYVLFAAGVPVWMFLQSRDFI
                     NVHMLYVGIIFLAVALIAAAVRGGGEIAGASAIAMNNWSGGSAKLGPGWPVLFVTIAC
                     GAVSGFHSLCAGGTTCKQLEKEKAARHVGYFGMLLESLLAVCVVCALIVGLSMQSYST
                     YCYPTAGKGNPVLTFAMGVGYTAHIGLGIPIAAGALGAMLLLEGFLVTTLDTAVRLTR
                     YMIEEGWSTLFARYDVFAATASGSPISEAQVGEVAGSGGLSPDKVAAASQARFQVIAT
                     HGLLNAVLRCLRLYWVNSGIAVALMLLLAMRGYAAVWPIFGSANQLLAALALIVATVW
                     LMAHRRAVWYTLLPALFMLVTSVWMLVRLLFTSYWPVLTTWPKKPALTVVAIIVLVAT
                     VAVVTLALRRWLAERANARGAFPIQP"
     gene            complement(4004..4606)
                     /locus_tag="P5316_15570"
     CDS             complement(4004..4606)
                     /locus_tag="P5316_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86427.1"
                     /translation="MGASKADSGSPAIQVPLRHRVLAVFTANVAVAFALAGWYGSEQY
                     VLKINSSRVLEVDMLRLEIKILTTRPGFHPDDGIRREQERAALNEQIGTTFIIAQAWK
                     WVMYLTAAILETTAIMIILGRRPLTAQIIASLAILVSTCCTVIAMHLLVRPDLGGMPS
                     LPTRSYLYLALIQGGYGAVLLVVTLSQRKPAARQCLSTVP"
     gene            complement(4619..6025)
                     /locus_tag="P5316_15575"
     CDS             complement(4619..6025)
                     /locus_tag="P5316_15575"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRR86428.1"
                     /translation="MSHEQMRIESDSMGTMQVPGWALWGAQTQRAVENFPLSGYRFDR
                     RFIRAMGLIKQAAAEVNHDLGLLDDERAALIVQAAQEVIDGKLDDHFVLDIFQTGSGT
                     STNMNTNEVISNRAIQLAGGRVGSRDPVHPNDHVNMGQSSNDVIPTAIHVAAAEGLKE
                     ALIPALERLASRLEEKAKALWPVIKIGRTHLQDATPIRLGQTFSGYAAQARLSAERAR
                     KAVTALRELPIGGTAVGTGINTHPEFGRRMAKRLSEQTGIEFVEAANHFEAQAAKDGV
                     CEASGLLRAVAVSLTKIANDIRWLASGPRCGIGELKIPATQPGSSIMPGKVNPVMSEM
                     LIQICAQVIGNDTAVAVGARDSIFELNVMMPMIARNLLESIRLLAGGVRVFTDRCVAG
                     IEADAERCKQMIEQSLAMCTSLAPVIGYDQAAAIAKEAYQTGRTVREVALAKGVLSET
                     DLHRLLDPASMTEPSAGK"
     gene            6480..7448
                     /locus_tag="P5316_15580"
     CDS             6480..7448
                     /locus_tag="P5316_15580"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR86429.1"
                     /translation="MTWVTAGRRYRSHNRGQVVRDRRAGARKGIKRAPNMSRAFTLIE
                     VLVVISIIGYLIALLLPSLSNARELSRSAVCAHNQHQLAVAATAYTVANADWMNPIED
                     WWPSSTDPDRVEVTFRVILFPYAGRSQHVFDCPAERMYIYSDGFSQADEDRTRSLNGP
                     TTSDRERYPYIYGIKHPLERWNFGGIGVAGVHWVGKKQAALPKSIPFGRAIESGYAEG
                     LRKYAQVKVPSRLIWYGDGAGYDGVVAKYGDDLGWWIRPKAIDGTQCNPGFNRLADEN
                     YGCQRHNKKANYAFADGHVDRLSANDIPCNSSECWWSYVPGYHRLP"
     gene            7486..8019
                     /locus_tag="P5316_15585"
     CDS             7486..8019
                     /locus_tag="P5316_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86430.1"
                     /translation="MSSRTKAIIKLSVAAGLAIVAVATAISKMRLLVASGEEGLHVWF
                     YDQSEAVLYTVPRGTYPPHEGVGGEKNDGVRAVVVAARDECNESSKRRIAYLETYTPE
                     YKQLVEGIRAARAEGRAYGRPIPSAESGFYEKNTLVRRVDDSRWFDMTTVEARQIVSQ
                     WRNERGPEGKTLDVCTP"
     gene            8056..9021
                     /locus_tag="P5316_15590"
     CDS             8056..9021
                     /locus_tag="P5316_15590"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRR86431.1"
                     /translation="MTTWFMVHAVITLGLPAASAIAAPEESSELGRARAMIEKARITM
                     VQAVEIAAQEVKDGKPLRAEIQMEDGQAQYEVTMIAGTQVVEVEIDAMSGKVLNVERE
                     EMEMTASHRWTFDREPAGALPPNWVIKQNNPTKEPAKWVIEPDADAASKPNVLNVKTA
                     NDNATFNLAIIENAVYRDLNLSVKVRGNTGADDQGGGLIWRCKDENNYYLCRINPLEN
                     NYRVYKVVDGKRTMLQSVDFETPAGKWFTLRVRMKGNRIACYCDGRKRLEVKDDTFKD
                     AGMIGLWTKADACSSFDNLQAYPISAGMPGEEEQTPGQVVVPTRP"
     gene            complement(9201..10118)
                     /locus_tag="P5316_15595"
     CDS             complement(9201..10118)
                     /locus_tag="P5316_15595"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRR86432.1"
                     /translation="MSLITGFLGSGKTSLLQHIVETNPHRRFVYVVNDCGAIDIDGRI
                     LQLGHNRLVNIPGGSIFCRCVAGEFVDWLTKIPKQWPEAGGPIEGVVIEASGIADPKV
                     IQQMLRETGLDRVYDLRMIIAVVDPGSFLKLIHILPNIVAQIEASQVILVNKTDIYDE
                     NRIRETEGEIRTMNAAARIVRTQHGRVELDVFAKTPVRTLEGRYARCLDPNYSVTTAA
                     VNGTVDPERLAADLRTLGDRVYRVKGFVLSSAGWIYVDLAAGRLTTRPAPTTGPATLV
                     FISSPQAQPAVDRIVTGLRTGAYEAQPRT"
     gene            complement(10124..>11169)
                     /locus_tag="P5316_15600"
     CDS             complement(10124..>11169)
                     /locus_tag="P5316_15600"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR86433.1"
                     /translation="KGRFPLVAEAMAELRRRFSDEIAFYGLITGPFTLALHLMGTGIF
                     LKMFDEVDAVREVLDFCTDVGRQAADFYLDNGADVIAVVDPMTSQISPRHFTEFVTSH
                     VNAIFAHVRQRKALSSLFVCGNATRNLELMCQTVCDNVSVDENISFELLRDLAHKHNK
                     SFGGNLKLTTVLLLGTPADAKRDALRCIDAAGSTGFILAPGCDLPYATPEENLQAVAA
                     MVHDEYQRNVARVACQEVTPDVFEEVQLPDYTREDKVIVDVVTLDSASCAPCQYMVDA
                     VEQAARKFPDQVVIREHKITTRSGLGHMAKLGVGQIPTICIDGQVKFPSIIPDINTLI
                     DAIETRAKEKRDK"
BASE COUNT         2206 a         3321 c         3565 g         2077 t
ORIGIN      
        1 atctctcgca acgtctgcag gttgccctcg gcaatgagtc gaccctcatg aagcaagccg
       61 atgcggtcgc aaagggcttc cgcctcatcg aggtgatgcg tactgagcag gatggccttg
      121 cccagctcgc gctcgtgacg gatgaaatcg agaatcagcc ggttggtcag cacatcaagg
      181 cccaaggtcg gctcgtccat gaccagcacg ggggggtcgg cgatcagggc ccgggcgatg
      241 ttggttcgct gcttctggcc cgtggacagc gccccgcagc gcagttcggc gaagtccgcc
      301 attcccagcc agtcaatgag ctgatcgact cgcctggtga tcaagccgcg ctccaggccg
      361 ttcaactccg caaagtagac caggatttca cgagccgtga gccggtggta gaggcccgtg
      421 ccggcggtga ggaagccgat gaatccgcga accgaggaag ggtctatggt gacgtccacg
      481 ccgttgacga tggcccgacc ggcggtgggc cgcatgaggc cggagacgat ccgcagggtg
      541 gtggtcttcc cggcgccgtt gggaccgagg aggccgtaga tctcgccggc attcacgtgg
      601 aagctcaagt gatccacggc cctcttgggc ggaccgatcg gggtggggaa ctccttggtc
      661 aattccagga cttcaagcaa gggcacgaaa acgcctccaa catcgcagcg gtcggccggc
      721 attgtcgtgc ctccggcgac gattgacaag aaaagggagc ccgccagccc gcatgggtag
      781 gtacgaagcg gacggccgaa ggttcacgtc ggatcgccgc cgcagcgggc gcatcttgtt
      841 ttcaggcatc ggttttatcc gtccgccgct gctgtgggat gtgttctgcg tcaggcggag
      901 ggttcggcag ataagtcgcc ggaggcatcg ccaccggatc ggctggtgaa ggcgccccct
      961 gcgtccggaa ggcgggttgg ggcagttgtg ctcgggcagc aatcgagtta acgtctgatc
     1021 aggatgagcc tcaatgtgaa aggagaatct gatgacaact cgtcgtatca tcatcgttgt
     1081 ggcggcaagc ctgtgtctcg tcggtttgtc ggccgtggtt cccggttttc cgcaagccag
     1141 cctggccgct caagttccag ccgagccggc ggcctcaccg gcgtcatcgg ggccggtcaa
     1201 gaccctgctg gtcaccggcg ggccgatcca tgacggcaaa gccatcggcg atctggtcga
     1261 ggagacaatg aagaagagcg ggctgttcga cgtgacgcgt gctcacgagg acctcgatgc
     1321 ctttctggcc gatcgcattg ccccgtttga tctggtcgtc ttctactaca cccttggcga
     1381 actgaaagaa gcccagaaac gcggactgat gagccatatc gcttcgggta aaggctacgt
     1441 gacgttccat tcgggggcag actcattccg cggcgatccg gattatcagg cattcgtcgg
     1501 cggctatttc aggacgcatc cgcactatcg gcaataccag gtcagcatca cggagaatga
     1561 cagcccgatc accaagggca tcgacgagtt catgatcacc gacgagcagt acatcctcga
     1621 ctacgatccg cgggtgacgg tactggccaa cgctttgttc aagggcaagc tcatgccggt
     1681 gatgtggacc aagccctggg gcaaggggag agtgttctat ctggcgctgg gccacgatgt
     1741 caaggcctgc cagcaggaga tgttcaagaa gctgctgctc cgcgggtctc tttgggccgc
     1801 cggccgaccg gtggtcgatc cgaagtgaat ttcacggctg aatggggaat gcaccgcgag
     1861 catttgccct ttcggccagc caccgccgca gagcgagagt caccaccgca acggtggcca
     1921 cgagtacgat gatcgctacc accgtcaagg ccggcttctt cggccatgtg gtgagcaccg
     1981 gccagtagct ggtgaacagg agccggacca gcatccagac ggaggtcacg agcatgaaca
     2041 atgccggcaa cagcgtgtac cacaccgccc gacgatgcgc catcaaccag acggttgcca
     2101 cgatgagcgc caacgcggcc aggagttgat tggcagagcc gaatatgggc cagaccgccg
     2161 cgtagccgcg catcgccaat agaagcatca atgcgactgc aatcccggag ttgacccagt
     2221 agagccgcag gcaacgcagc actgcgttca ggagcccatg agtcgcgatg acttggaagc
     2281 gtgcttgcga ggctgcggcc accttgtcag ggctcagccc tcccgatccg gcgacttcgc
     2341 cgacttgggc ctcactgatg gggcttccgg aagccgtcgc ggcaaagacg tcgtagcggg
     2401 cgaacagcgt cgaccagcct tcttcgatca tgtagcgggt cagccggacg gcggtgtcca
     2461 gcgtggtcac caggaacccc tcgagcagca gcatcgcgcc cagggccccg gccgcgatcg
     2521 gtatgcccag gccgatatgg gccgtgtaac cgactcccat cgcgaaagtc aggacaggat
     2581 tgcccttgcc cgccgtcgga tagcagtacg tgctgtagct ctgcattgac aatccgacga
     2641 tgagtgcgca gaccacacaa accgccaaca ggctttccag aagcatcccg aagtagccga
     2701 cgtgacgggc ggctttctcc ttctcgagtt gcttgcacgt cgtaccgccc gcgcacaggc
     2761 tgtggaaacc gctgactgct ccgcacgcga tggtcacgaa gagcaccggc catccggggc
     2821 cgagcttggc gctgcccccg ctccagttgt tcatggcgat cgccgatgct cccgcgatct
     2881 cgccgccgcc tcgcactgcc gcggcgatga gtgccaccgc aaggaagatg atgccaacgt
     2941 acaacatgtg gacgttgatg aaatcccggc tttgcaggaa catccatacg gggacgccgg
     3001 cggcgaagag cacgtatata ctgatcagca gcttccaggt catgggttca acggccattg
     3061 gcaggtatag gccgatcgct accgacacgg cacagatgag gatcgccagg cccgagcaca
     3121 gccagaccgg ccagcgtcgc ttgatgtaca agaaaccgat cagcggcgag aaggcggtaa
     3181 tcaccacgac ggaggtcgac gcgatgcccc cgatgatcgc gtggggctct tcctggccgg
     3241 tcatgtcgtt tacccatgag acttcgcgaa atatcgtttc gccgggctgc atcttgagcg
     3301 tggccacggg caccttggag gtaagcgcgg tggcggaaag gtcgagaaac gcggcgcaca
     3361 ccagcaccag cagagcaacg agcagaacca acatcatcag aaaggcggca ggtccgacgt
     3421 agcgacgggc aatcacggtc aggtttttgc cgccttcgcg catggcgatg tgggccacca
     3481 cgtaatcgtg gaccgccccg agaaacacgc ctccgagcag gatccagatc aacgccggtc
     3541 cccagccata gagcaaggcc agaaccgggc cgatgatcgg accggcaccg gcgatcgatg
     3601 cgaagtgatg ggcgaacaca atgggcgtgc gggtcggcac gtagtcacgc ccgtcgttta
     3661 tggccactgc cggggtgacc cgatcggccc gctcgccgag tacccgtccg atgatgggcg
     3721 cgtagatcca gccgcccagc agaagaaaca ccacggacac gcctaggatg gccagcacga
     3781 tcatggaaga cctccgaccg gaatggaact gttcctttca gcataacatc ggcaatcacg
     3841 ccaagtccag atcatcgctc cccacttgtg tggcggcacc gccgtcggga cagggacgcg
     3901 tccgccttct ggagaatcgg gcgcgatcgt tgcggcaagg tgtaccacgg ccgggcaaac
     3961 agggggccga atcaggcgga atgcgaggct ggtcgtgtca ctttcaggga acggtggaaa
     4021 gacactgccg tgctgccggt ttccgctggc tcagggtgac gaccagcaag accgctccgt
     4081 agccgccctg gatcagggca aggtacagat acgagcgggt gggcaagctg ggcatgccgc
     4141 ctaaatcggg ccggacaagc agatgcatcg cgatgaccgt gcagcacgtg gaaaccagaa
     4201 tcgccagtga cgcgatgatc tgtgctgtca gaggccggcg cccgagaatg atcataatcg
     4261 cggtcgtctc caggatggcg gccgtcaggt acatcaccca tttccacgct tgggcaatga
     4321 tgaatgtcgt gccaatctgc tcgttaaggg cagcgcgctc ttgttcgcga cgaatcccgt
     4381 cgtccgggtg aaatcccggt cgagtggtga ggatcttgat ttccagccgg agcatgtcga
     4441 cttcgagcac tcgcgaggag ttgatcttca gcacgtactg ttccgagccg taccagccgg
     4501 ccaaagcaaa ggccactgcg acatttgcgg tgaaaacggc gagtactcgg tggcgaagtg
     4561 gaacctggat tgccggactg cctgaatctg ccttggatgc gcccatgcgt ctccccgttc
     4621 attttcctgc cgacggctcg gtcatcgagg ccgggtcgag taatcgatgc aggtcggtct
     4681 ccgacaaaac acccttggcc agagcaacct cccgcacggt gcggccggtc tggtaggcct
     4741 ctttggcgat cgcggccgcc tggtcatagc cgatcaccgg tgccaggctg gtgcacatgg
     4801 ccagggactg ttcgatcatc tgcttgcacc gctccgcatc ggcttcgatg cctgccacgc
     4861 atcgatcggt aaacacccgg acgccgccgg ccaggagccg gatcgattcg agcaggttcc
     4921 tggctatcat cggcatcatg acgttcaatt caaaaatgct gtcgcgtgct ccgacggcta
     4981 ccgcggtgtc atttccgatc acctgggcac atatctggat gagcatctcg ctcatgaccg
     5041 ggttaacctt gccgggcatg atgcttgagc cgggctgggt ggcgggtatc ttcaactcgc
     5101 cgatcccgca cctcggcccg gaggccagcc agcggatgtc gttggcgatc ttggtcaacg
     5161 acacggccac cgctcgcagc agcccgctgg cctcgcagac gccgtccttg gccgcctggg
     5221 cctcgaagtg gttggccgcc tcgacgaact cgattccggt ttgctcgctg agccgtttgg
     5281 ccattcgccg accgaactcc gggtgggtgt tgatgcctgt cccgacggcc gtgccgccaa
     5341 tgggcagttc ccgaagggcc gtcacagcct tccgtgcccg ctcggcactg agcctggcct
     5401 gggccgcata gccgctgaac gtctgcccga gccggatggg ggtggcatct tgcagatggg
     5461 ttcgcccgat cttgatcacc ggccagaggg ctttggcctt ttcctccagt cttgaggcca
     5521 gcctctccag tgccggtata agagcctcct tcaagccttc ggctgccgcc acatggatcg
     5581 ctgtcggaat cacgtcattg ctcgactgcc ccatgttaac atggtcattt ggatgaaccg
     5641 ggtcgcgcga tccaaccctc ccgccagcga gttgaatggc ccggttggag atgacctcgt
     5701 tggtgttcat gttcgtgctg gttcccgagc cggtctggaa gatgtccaac acgaagtgat
     5761 catccagctt gccgtcgatg acttcctggg cagcctgaac gatcagggcg gcccgttcgt
     5821 cgtctagaag ccccaagtcg tgattgacct ccgcggccgc ctgtttgatg aggcccatcg
     5881 ctcggatgaa gcgccggtcg aaccgatacc cgctgagcgg aaagttttcg accgcccgct
     5941 gggtctgcgc cccccacaag gcccagccgg gcacctgcat tgtgcccatg ctgtccgact
     6001 ctattcgcat ttgctcgtgg ctcatcgttg agtcctcgct tggcgaccgt tgaccggccg
     6061 acagaacagg cattgtccaa gatcgggcgg catttgacca gcgccggctc ctgtggcggg
     6121 tttgacggtt tggacggggg tcaaatagat ggagtgccgg aacagacggc cgatggtgac
     6181 atgccaagaa tcagaaaacc acctatccgg ctgcatattg cgggtcggcg aggcagacgg
     6241 tagtatttac gagccggtga cgcagcaggg accgattctc acgcatccgt gaggtcggct
     6301 gccgggcgtg cttccggatt tcggggttct ggaccaactg tgcggggctg aggggggaaa
     6361 gggccatcgt ggtattcttc cggtcacgga tgacagcatg tttccagaac ctctggcggg
     6421 ttcggggctg agcattttct tgcatggagg acgcaacctc ttatggggag gacgcaacaa
     6481 tgacatgggt gacagcgggc cggcgatatc ggtcgcacaa ccggggtcag gtggttcgcg
     6541 atcgccgcgc gggtgcgcga aaggggatca agcgggcgcc caacatgtct cgcgcgttca
     6601 cgctgatcga ggtgctcgtc gtcatctcga tcatcggcta cctgattgcc ctgttgcttc
     6661 cgtcactgag caatgcgcgg gagctatccc gcagcgccgt ttgcgcccat aaccagcacc
     6721 agctcgccgt ggctgcgacg gcttacaccg ttgccaacgc ggattggatg aaccccatcg
     6781 aggactggtg gccttcatca acagaccccg atcgcgtcga ggtcacgttt cgggttattc
     6841 tgtttcccta cgcggggcgc tcccagcacg tgttcgattg tccggccgag cggatgtaca
     6901 tctactcaga tgggttttcc caggcggacg aggaccgcac tcgttcgctc aatggcccca
     6961 ccaccagtga ccgggaacgg tacccctaca tctacggaat caagcatccg ctggaacgat
     7021 ggaatttcgg cggcataggt gtcgccgggg ttcactgggt cggcaagaag caagccgcct
     7081 tgcccaagtc gataccattt ggacgggcca tcgagtcggg ttatgcggag ggactcagga
     7141 agtacgcaca agtcaaggtc ccttcgaggc tgatctggta tggtgacggc gcaggctatg
     7201 acggtgtcgt ggcgaagtac ggtgatgacc ttgggtggtg gatcagaccg aaggcgatcg
     7261 acggcacgca atgcaatccc gggttcaacc gactggcgga cgagaattac ggttgtcaac
     7321 ggcacaacaa gaaggccaac tacgcctttg ccgacggcca tgttgatcgg ttaagcgcca
     7381 acgacatccc gtgcaactcc agcgagtgtt ggtggtcgta cgtcccgggc tatcaccgtc
     7441 tgccgtgacg gcccgtgtcc tactgaaccg cgtgtggacc ggcatatgag ttccagaacc
     7501 aaggcgatca tcaaactgag cgttgccgca ggattggcga tcgtcgcggt tgcgacggcg
     7561 atctccaaga tgcgcctcct tgtcgcgagc ggcgaggagg gtttgcacgt ctggttctac
     7621 gaccagagcg aagccgttct ctataccgtc ccgcgcggga cttatccgcc ccacgaggga
     7681 gtcggcggcg agaagaacga cggcgttcgg gccgtcgtgg ttgccgcacg cgacgagtgc
     7741 aatgaaagct cgaaacgccg gatcgcatac ctggagacct acacgccgga gtacaagcag
     7801 cttgtggagg gcattcgtgc cgcccgggct gagggccgag cctacggccg gccgatccca
     7861 agcgcggaaa gcggcttcta tgaaaagaac acccttgttc gtcgcgtcga cgattcgagg
     7921 tggttcgaca tgacgacggt cgaggccagg cagatcgtct ctcaatggcg aaacgagcgc
     7981 ggcccggaag gcaagacgct ggatgtgtgc acgccttgac cggtttgagt gttgtttctt
     8041 tgcaggagaa tacccatgac cacgtggttt atggtacatg ccgtcattac attgggtttg
     8101 ccggcggcgt cggcgatcgc ggcgcctgaa gagagttccg agctggggcg ggcccgggcc
     8161 atgatcgaaa aggccaggat caccatggtt caagccgtcg agatcgcggc ccaggaggtg
     8221 aaggacggca agcctttgcg ggccgagata caaatggaag acggccaggc gcagtacgag
     8281 gttacgatga tcgccggcac gcaagttgtg gaagtcgaaa tcgatgccat gagcggaaag
     8341 gtgctgaacg tcgagcggga agaaatggag atgacggcga gccaccgctg gacgttcgac
     8401 agggagccgg ccggcgcttt gcctcccaac tgggtgatca agcagaacaa tcccacgaaa
     8461 gagccggcca agtgggtgat cgagcccgat gccgacgccg cgagcaaacc caacgtgctc
     8521 aacgtaaaga ccgcgaacga caatgccact ttcaatctgg cgattatcga gaacgccgtt
     8581 tacagggatc tcaacctcag cgtcaaggtt cgcggaaaca ccggtgcgga cgatcagggc
     8641 ggagggctta tctggcgttg caaggatgaa aacaactatt acctctgccg gatcaacccg
     8701 ctcgagaaca actatcgcgt ctacaaggtg gtcgacggca agcgcaccat gttgcagtcc
     8761 gtggacttcg agacgcctgc ggggaagtgg tttaccctcc gcgtgagaat gaagggcaac
     8821 cggatcgcct gctactgcga cggcaggaag cggctcgaag tgaaggacga cacgttcaaa
     8881 gacgccggaa tgatcggact gtggaccaaa gccgacgcct gctcttcttt cgacaatctg
     8941 caagcctatc cgatcagtgc cgggatgccc ggggaggagg aacaaacgcc gggacaggtc
     9001 gtcgtgccga ccagacccta aggcggaccg cctttctgcg ggccgcgccc caaacgttct
     9061 gcgatccaga aacatgcgcg cacaatgcgc accagttgcg acaaaagaga ctgacggcag
     9121 gaaccgcgaa aggggcgggg atgatcgttt tctacgcgga aggtgaacgg gccacttctc
     9181 gtcgcgccgg tcactccggg tcatgttcgc ggctgtgctt cgtacgcgcc ggtgcggaga
     9241 ccggtcacga tgcggtccac ggcgggttgt gcctgcggcg aactgatgaa taccagcgtg
     9301 gccgggccgg ttgtgggcgc gggtcgtgtg gtcaaacggc cagccgcgag atccacatag
     9361 atccaaccgg ccgacgagag cacgaagccc ttcacgcgat atactcggtc gccaagcgtt
     9421 cgcagatccg cagccagacg ctccggatcg acagtcccgt tgaccgccgc cgtggtgacc
     9481 gagtagttgg ggtccaagca gcgggcataa cggccttcaa gcgttcggac gggggttttg
     9541 gcaaagacat ccagctcaac gcggccgtgt tgggttcgca cgatcctagc tgcggcgttc
     9601 atcgtccgga tttcaccctc ggtttcccga atccggttct catcgtaaat gtcggtcttg
     9661 ttcaccagga tgacttggct ggcctcgatc tgggccacga tgtttgggag gatatggatg
     9721 agcttgagga aactaccggg atcgaccact gcgatgatca tccgcaggtc gtagacgcga
     9781 tcgagtccgg tctctcggag catctgctgt atgaccttgg ggtcggcgat gccgctggcc
     9841 tcgatgacga cgccttcgat cgggccgccg gcttcgggcc attgcttcgg gatcttcgtc
     9901 agccagtcaa caaactcgcc cgcgacgcag cgacagaaga tgctcccgcc cgggatgttg
     9961 accagccggt tgtggccgag ctgaaggatg cggccgtcga tgtcgatggc cccgcagtcg
    10021 ttgacgacat atacgaaccg gcgatgcggg ttggtctcga cgatgtgttg cagcagcgag
    10081 gtcttgccgc tgcctagaaa ccccgtgatc aggctcatgg ggatcacttg tccctctttt
    10141 ccttcgctct ggtctcgatg gcgtcgatca gcgtgttgat gtccggaatg atcgacggga
    10201 atttcacctg gccgtcaatg cagatggtcg ggatctggcc tacgccgagt ttggccatgt
    10261 gtcccaggcc gctgcgagtg gtgatcttgt gttcgcgaat gaccacctgg tccgggaact
    10321 tccgagcagc ctgctcgacg gcgtcgacca tgtactggca aggcgcgcag gaggccgagt
    10381 ccaaggtcac cacgtcgacg atgaccttgt cctcccgcgt atagtcaggc aattggacct
    10441 cctcgaacac gtcgggcgta acctcttggc aggccacgcg ggcgacgttg cgttgatact
    10501 cgtcgtgcac cattgccgcg actgcctgca gattctcctc cggcgtggcg tacgggaggt
    10561 cgcagccagg cgcgaggatg aagccggttg atccggcggc atcgatgcat cgcagggcgt
    10621 cgcgcttcgc gtcggccggc gtccccagca acagcacggt cgtcagcttg aggttgccac
    10681 cgaaagactt gttgtgcttg tgggccaggt cgcgaagcag ctcgaaggag atgttctcgt
    10741 ccaccgaaac gttgtcgcag accgtttggc acatcagctc cagattgcgg gtggcgttgc
    10801 cgcagacaaa aagagaggag agggcctttc gttgtcgcac atgcgcgaag atcgcgttga
    10861 catggctggt cacgaactcg gtgaagtgac gcggtgagat ctggctggtc atcgggtcga
    10921 ccaccgcgat gacgtccgcg ccgttgtcca ggtagaaatc ggcggcttgc ctgcccacgt
    10981 cggtgcagaa atcgagcacc tcacgcaccg catcgacctc atcaaacatc ttcaggaaga
    11041 tgccggtgcc catgaggtgc agcgccaggg taaacggacc ggtaatcagg ccatagaagg
    11101 caatctcgtc gctgaatcgc ctcctgagct cggccattgc ctcggcgaca agggggaagc
    11161 gtcccttgg
//
LOCUS       DAOTPW010000185        11147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5515_length_11147_cov_6.664172, whole genome shotgun sequence.
ACCESSION   DAOTPW010000185 DAOTPW010000000
VERSION     DAOTPW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5515_length_11147_cov_6.664172"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1815
                     /locus_tag="P5316_15605"
     CDS             157..1815
                     /locus_tag="P5316_15605"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRR86434.1"
                     /translation="MTVVVVVVALVTAEYYTARPDFCGSCHIMEPYYQSWTRDVHGSK
                     LSVACVECHYAPGEQHTIRAKFRGLAQAAGYFSGRSGSGRLRTHVSDASCLTSECHGD
                     RVYLNKALPVGEAGTETRLVSSRETEVARTPTVSFVHSKHLQVATRLDETTKALERTT
                     ARLKTALGENETYVALAREAASVASSPERDARIRERVRQTGSAAVEADALAWGRLERL
                     KIRLRQLSGMTCAACHTYDASAKQHQAVDRQVCFTCHFSHEAFNKETAECLRCHEAPT
                     RRIAVHGWTSSQPSAVMMDHQEIVRRNIDCSSCHADVVKGRGAVTIRECSQCHHQERY
                     TAEFAERTIRQVEDYHRVHVAAQNARCADCHETIRHELAAPIMTASHEGFLQVVLENC
                     QHCHPNHHHEQVQLLAGIGGRGPTLAMPNAMFGSRTNCRACHTQPGSDLKGASVITAT
                     ASACVACHNESYGSTLDQWIHEIAVRLKECDNSLKQLDKRIEQARSQGRDVPPRVLEL
                     AAQVRANVQFLQLGNGIHNRNYALVLLDISEAGLRQAAGLLPEP"
     gene            complement(1936..2916)
                     /locus_tag="P5316_15610"
     CDS             complement(1936..2916)
                     /locus_tag="P5316_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86435.1"
                     /translation="MRGELCNIVLSGVLAVTVAGVITVSSAEPTSTAPASSQPAGRAE
                     NPHWSTDGCRHCHQIRKDRTFSIEQSRINDVCRQCHDGTQAAREVHPVGRLFAGDQVR
                     RPDNWPAPGGALSCVTCHDIRRACDYPQPRPALNGSFLRPVSAQGPMAFCAECHIAAF
                     QAGGGRLNVHVMLDGAGQRNDDACRFCHLPSFDLDAPPTRTGKPLLRADGISLCVSCH
                     PNHLDYFEPGHIGHEVAEPMRTLMPALEPILWAGRPLFPQGQPDGRALTRLPLENEQR
                     IVCATCHNPHQEGTFPEWSVLADGAIPLVREEPQVRLRGIEKDLCYACHE"
     gene            complement(2906..4042)
                     /locus_tag="P5316_15615"
     CDS             complement(2906..4042)
                     /locus_tag="P5316_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86436.1"
                     /translation="MPVLPHQVTRSLPRAVIGLAISAILPTAGCVSLHRADKHTFEPL
                     HFPSADAWPRLVLYGNLSHLLPTFDRTPPLELWLYGPSSFGKTAMRNPQGMALLGTRL
                     LVCDQGRSDIIAVDLADGSSLIWGDPDHRPRCPVDIAVGAPDSVYVADANLRSVLHYG
                     VDQRFRGEIKPAEASDERFRPAALCVGDNILYVGNTSGHQIDRWDLTTRQWLDSLVPP
                     KSQPNLVAPTGLAFTRNGVLLTVDAIGARVFRYRNDGRWLDPIGRPGRGQGELIRPKQ
                     VCVTAGGLMLVTDAGRQSVVVFDGDGRFVTEIREQSEAWRGLTLPFGILALSPERMPA
                     PPQAAVRETADPTAYIVVSDSLGRDSLLLLGVFESPVQESQRAR"
     gene            complement(4018..4683)
                     /locus_tag="P5316_15620"
     CDS             complement(4018..4683)
                     /locus_tag="P5316_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRR86437.1"
                     /translation="MRLLFTVLLLFLGISSGGAVDRPASPESRPDDAGGRVADLRRSL
                     VGSKHDFTDVLGGAGTACSACHVPHMMAVRPASQPGGGVLTTQPATVEVYRIAGQRRV
                     FAVDRYTPGPTSLVCLGCHDGTVATSTMGSGHALLAGVREGFAVPDEFVWRDHPVGVP
                     YPSNRREYRAESMVTAEGKIPLPDGRVECISCHDPHNRSGVDKMLVMSNRRSALCLSC
                     HIK"
     gene            complement(4734..6500)
                     /locus_tag="P5316_15625"
     CDS             complement(4734..6500)
                     /locus_tag="P5316_15625"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRR86438.1"
                     /translation="MRIGRWTLLALWAIGGGDLLSAGEQPRSSAAGRQTVVGSPHDLS
                     VGGPGRIHAMEEEQVCIFCHTPHNATGQRPLWNRHMPPTHYRIYESSTTDARIDQPSG
                     PSKMCLSCHDGSLAIGLLAGRPDDIVVMSRRTMALGRGNLSLDLSDDHPIGFRYDRAL
                     AGRDHQLHDPGVISRELPLGKHGEMHCTTCHDPHNNHLGNFLRITKVRSAICLSCHDR
                     DGWGVASHATSRAVIRGRAVDMRERLPFASVAENACGNCHKIHSAGGRERLLRFANLE
                     DNCLNCHNGSVASTNIESEICKPSAHNAPLLFDCHDPVEDPLTMSRHVTCADCHNPHA
                     AAYDFISRPGATAPEPINNRMRYVSGVNILGRPVNEAMREYEVCFKCHADNPSRPQNA
                     VITRDIHQTNTRLEFQPTNPSFHPVAAPRNNHDVVSLTSPWRVGSLTKCTDCHNSDAG
                     SALSLNRRAGPHGSIYRPLLIANYSTRDYTVESVIAYALCYRCHERTSVLNNESFPLH
                     SRHVVNGRSPCSACHDAHGISRTQGSSRNHSSLINFDRSIVQPASGGLGARIEYEDRG
                     TYRGSCTLTCHGVVHVRFEYAR"
     gene            complement(6497..7531)
                     /locus_tag="P5316_15630"
     CDS             complement(6497..7531)
                     /locus_tag="P5316_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86439.1"
                     /translation="MIKAHGYTARPGAAIILFAVSGCSQVAVSPLTPPSPPVVWPKPP
                     DVARVQYLGCLAGSQSVRAKKSLSERWDELLHGPKSPEVFVTPHAVAVHPDCRRVAVA
                     DTNGACVHLLDLEAGKYERLTTIGRSDRLQCPVGVAWVGASIWTADAKAGAIAVLGCP
                     TSRWIRNESLERPAGIAFCPENGLCYVSDSAGHALLAFDERGVCVLRFGTHGNGPGQF
                     NGPSHLACGPGPALVVADSLNSRLQYLGPDGAFIAQIGKKGDAAGDLSLPKGVAFDPR
                     GNIWVVDAHFENVQAFTPDGRLLMSLGHEGQGIGEFSLPAGLTIDARGRIWVADTYNR
                     RVQVFELLPS"
     gene            complement(7528..9612)
                     /locus_tag="P5316_15635"
     CDS             complement(7528..9612)
                     /locus_tag="P5316_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86440.1"
                     /translation="MRVEPGIKAIATLLQIVHPLFALVALGQAPPTTAPAAGETPVSG
                     ERRLFGINLTDLYVGIEGEYAQRRVRYGGETSDYLHENRDFRLYELVGAGLAGHIYDA
                     NLIEYRATLEFGLSQTRFTEQADGVSREENQSDGFLHEYDVSLDVLKAKPVSFSVYAR
                     RSDHRLPRRFLPSLHEIDTSAGISSLIIAGPTTTELGLEWSDVRRRGNRDRLDDEELE
                     VSRFYLDHTWAISQDQKLRVQYDHDREESNYQGDSYNYDSRRDELRLSHELAFGSAGQ
                     HRLDTLLRYGQEDGDWARDEIEFLPRLSLAHTDKLKTVYRYNLYRFQQDAIDITQHKF
                     DVEALYRPREDLRITVDTFGLYEAADDDVDSVEYGGGFDVSYVRPTSSGELTINLASA
                     FERTEVHGDAGRRYVLSEGHVLGGSRPVYLNQRGIVPGSILAHNERRTRYYVAGSDYL
                     VTDLGQLVRVERIPTGRIEDNEVVYFDYQYIIPAEASMNEFRNSFLVEHAFKFGLTPY
                     YTLESRCEDVNTSEATAWRRHDSHRHRLGSRFDRDRWSAGAEYELYDDSTEPYDACHF
                     TGRASLIRTADHSLDVTGELSRYWFEGGWDRRRVWWLDIDVKDRFRINQMLALKNGLG
                     YRWEDDTVDGTTHGIDLECGFQYVRGALTVDLTVEYDLLSIAGNRDGGFGVYLNVRRN
                     LTHLLPRRGDLP"
     gene            10238..10900
                     /locus_tag="P5316_15640"
     CDS             10238..10900
                     /locus_tag="P5316_15640"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRR86441.1"
                     /translation="MMDFNAESVRDSRRRARSLLFGLAVSALALGQRCVPIQPQACVP
                     ELADDDHVLAVGRPAVTVFEYANFECSHCGQFARETWPTLRQEYVDTGKVRWIARHRP
                     FRSAYAPVGVAAQASECAADQGAFWEYRELVYANQADLSVAALKSYAVELALDTQAFD
                     ACLDGQVKAAEIDAERAAAEALGVCCTPYFFVDGRPFAGFRTVEEFRTILDAALAAAG
                     AD"
BASE COUNT         2147 a         3466 c         3462 g         2072 t
ORIGIN      
        1 gttttccaag aggcagacac catgaccggt tccgggcagg cttccgatgg aggtgcagaa
       61 tcgaggaatc cgcggactga tgagccggaa cctgcggggt tctctcggcc gtctcgccgg
      121 aggcggcgga tcaggcgact tggtcgtgcc ttggctgtga ccgtagtcgt ggtcgtggtg
      181 gcgctggtca cggccgaata ctacaccgcc agacccgatt tctgcgggtc ctgtcacatc
      241 atggagccct actaccaatc ctggacgcgc gacgtgcacg gctcgaagct cagcgtggcg
      301 tgcgttgaat gccactacgc cccgggtgag cagcatacga ttcgagccaa gttccgcggc
      361 cttgcgcagg cggccggcta cttcagcggc cgatccggct ccggccggct gcgcactcac
      421 gtcagcgacg caagctgtct gacttccgaa tgtcacggcg accgcgttta cctgaacaag
      481 gctttgcctg tgggtgaagc cggaaccgag acgcgcctcg tcagcagccg ggaaaccgag
      541 gtcgcacgca cgcccacggt cagttttgtg cacagtaagc acttgcaggt ggcgacgcgg
      601 ctcgacgaga cgaccaaggc ccttgaacgg acaactgcca ggttgaagac ggctctcggc
      661 gaaaacgaaa cctacgtcgc cctcgctcgt gaggcggctt ccgtggcttc gtcgcccgag
      721 cgcgatgcga gaatccgcga gagggtcagg cagaccggat ctgcagccgt cgaggctgat
      781 gccttggcgt ggggacgcct cgagcgcctc aagatccgcc tgagacagct cagcggcatg
      841 acatgcgccg cttgccacac ttacgatgcg tcggcgaaac aacatcaagc agttgatcga
      901 caggtgtgtt tcacctgtca cttcagccac gaggccttca acaaggaaac cgccgagtgc
      961 ctgcgatgtc acgaggctcc gacgcgacgg attgctgttc atgggtggac cagcagtcaa
     1021 ccctccgccg tcatgatgga tcatcaggag atcgtccgtc gcaacatcga ctgctcgagc
     1081 tgtcacgccg acgtcgtgaa gggccggggc gcggtgacga tccgtgaatg ctcgcaatgc
     1141 caccatcagg aacggtatac agcggaattt gccgagcgga ccatcagaca ggtggaagat
     1201 taccaccgcg tccacgtggc cgcccagaat gcccgatgcg ctgactgcca cgaaacgatc
     1261 cgacacgaac tggcggcgcc gatcatgacg gcgtcgcacg aaggattcct gcaagtggta
     1321 ctcgagaact gtcagcattg tcatcccaat catcatcacg agcaggtgca actcctggcc
     1381 ggcatcggcg gccggggacc gaccctcgcc atgcccaacg cgatgttcgg atcgcgtacc
     1441 aactgccgtg cctgccacac gcagcccggt tcggacctca agggggcgag cgtgatcacg
     1501 gcaacggcct ccgcctgtgt cgcctgtcac aacgaatcat acggctcaac gctggatcaa
     1561 tggattcacg aaatcgccgt ccggctgaag gagtgcgaca actcgctgaa gcagctcgac
     1621 aagcggatcg agcaggcccg atcccagggg cgtgacgtcc cgcctcgtgt cctggaattg
     1681 gctgcacagg tccgggcgaa tgtgcagttt ctgcagttgg gcaatggtat ccacaaccgg
     1741 aactatgcat tggtcctcct ggacatcagc gaggccggtc tccggcaagc cgccgggctg
     1801 ctgccggagc cgtaatgcct cgccaggcct ccgtagcgat ggcaactcac cctgttctcg
     1861 gcgaccgcgg ccggaaacgg ctccgatacc accccgatca gtggggctat tcgcaacatg
     1921 cccgattgat agccgttatt catgacaggc ataacacaga tccttttcta tgccgcgcaa
     1981 ccgtacttgc ggttcctcgc gaacaagcgg gatcgcaccg tcggcaagga cgctccattc
     2041 aggaaaggtg ccttcctgat gagggttatg acaggtggcg cacacgatgc gctgttcgtt
     2101 ctccagaggt aatcgggtca atgccctccc gtcaggctga ccctgcggaa acagtggacg
     2161 tcccgcccac agaatgggtt cgagagccgg catcagagtc cgcatcggtt cggcaacctc
     2221 atggccgatg tgaccgggct cgaagtagtc caggtgattc ggatggcagg aaacgcacaa
     2281 cgatatgccg tcggcccgaa gcaggggctt gcccgtccgt gtgggaggag cgtcgaggtc
     2341 gaatgagggc agatgacaaa accggcaggc atcatcattc cgttgtcctg caccgtcgag
     2401 catgacgtga acgttgagcc gacccccacc cgcttgaaag gcggcgatgt ggcattccgc
     2461 acagaaggcc atcggtcctt gcgcggaaac aggacgcaaa aatgagccgt tcagcgccgg
     2521 acgcggctga ggataatcgc acgctctgcg gatgtcgtga caggtgacac agctcagggc
     2581 gcccccgggt gcgggccagt tgtcgggacg cctgacctga tcgccggcga acagccgacc
     2641 cactggatgc acctcgcgtg cggcctgcgt cccatcatgg cattgccggc aaacgtcatt
     2701 gattctactc tgctcaatcg agaacgtgcg gtctttccgg atctggtggc agtgtcggca
     2761 gccgtcggtc gaccaatgcg gattctctgc tcggccggca ggttggctgg atgcaggagc
     2821 cgtcgacgtc ggctcggcgg aggagaccgt gatcacccct gcaaccgtaa ccgcaagcac
     2881 gccgctgagc acaatgttgc agagttcacc gcgcacgctg cgactcctgc acgggagact
     2941 caaagacgcc gagcagcagc agcgaatctc gcccaagcga gtcggacacg acgatgtatg
     3001 cggtcgggtc ggcggtctct cgcacggcgg cttggggagg agccggcatc cgctccggcg
     3061 acagagcgag aataccgaac ggcaaggtca gcccgcgcca tgcttccgat tgttcccgta
     3121 tctcagtgac gaagcggcca tctccgtcaa aaaccacgac ggattggcgg ccggcgtcgg
     3181 taaccagcat taacccgccg gcggtcacgc acacctgctt cggccgaatc aactcgcctt
     3241 gtcctcggcc cggacgccca atcggatcaa gccagcggcc atcattgcga tagcggaata
     3301 cgcgggcgcc aatcgcatcg acggttagca gaacaccatt cctggtgaat gccagaccgg
     3361 tcggagcgac cagattgggc tgcgacttcg ggggcaccag actatcaagc cattggcggg
     3421 tggtcagatc ccatcggtcg atctgatgac cgctggtgtt gcccacgtaa agtatgttgt
     3481 cgcccacgca cagcgcggcc ggacgaaatc gctcgtctga cgcctcggca ggtttgatct
     3541 cgccgcggaa tcgctgatct acaccgtagt gaagcactga tcggagattc gcatccgcga
     3601 cgtacacgct gtcaggcgct ccgactgcaa tgtctaccgg gcatctggga cgatgatcgg
     3661 ggtcgcccca gatgaggctg ctcccgtccg ccaggtctac tgcgatgata tccgatcgtc
     3721 cctggtcaca gacgagtagt cgagtgccaa gaagggccat gccttgaggg ttgcgcatcg
     3781 cggtcttgcc gaagctgctc gggccgtaca gccacagttc cagaggcggc gttcgatcga
     3841 aggtcgggag cagatggctc aggttgccgt acaggacaag gcgaggccac gcatccgcgg
     3901 aggggaaatg gagaggttcg aacgtgtgct tgtcggcgcg gtgaagcgaa acgcagcctg
     3961 cggtcggcag gatggccgaa atcgcaaggc cgatcacggc ccgcgggaga cttcgagtca
     4021 cttgatgtgg caggacaggc acagtgcgct cctgcggttt gacatcacca acatcttgtc
     4081 cacgcccgat cggttgtgag gatcgtggca cgaaatacac tcgacccggc cgtccggaag
     4141 cggaatcttg ccctcggccg tgaccatcga ttcggctcga tactcacgac gattgctcgg
     4201 ataagggact ccgacggggt ggtcccgcca gacgaattcg tccgggactg caaacccctc
     4261 ccggacgccc gccagcaagg catggccgga ccccatggtc gaggtcgcga cggtgccgtc
     4321 atggcagcca aggcacacca ggcttgtcgg ccccggcgta taacggtcca ccgcaaagac
     4381 gcgacgctgc ccggcgatgc gatagacctc cacagtcgcc ggctgtgtgg tcaacacccc
     4441 gcccccaggt tgagacgcgg gacgcacggc catcatgtgg gggacgtgac aggcgctgca
     4501 ggccgtaccg gcgccgccca ggacgtcggt gaagtcgtgc ttactcccca ccaggcttcg
     4561 gcgaaggtcg gcaacacgac cgcccgcatc gtccggacgc gattcgggtg aagcaggccg
     4621 atcgacggct ccgcccgacg atattcccaa gaatagcagc aaaaccgtga acaagagcct
     4681 catgtctgat ctctcatcta taaaggcctc ggcggcaacg ccgccgggca tgtttacctc
     4741 gcgtattcaa accgtacgtg aacgacgccg tggcaggtta gcgtgcaact gccgcggtaa
     4801 gtcccgcgat cctcgtattc gattctcgct cccagcccac cggaagcggg ctgcacaatg
     4861 gagcgatcaa aattgatcag gctgctgtga ttcctgctgc tgccctgcgt ccggctaatg
     4921 ccatgggcgt cgtggcaggc ggagcagggc gaccggccgt tgacgacgtg acggctgtgc
     4981 agcggaaaac tctcgttgtt caaaacgctg gtccgctcat gacatcgata gcacagcgca
     5041 taggcgatga cactttcgac cgtgtaatcc cgtgttgagt agttcgcaat cagtagcggc
     5101 ctgtagatcg acccatgggg ccccgcccgt ctgttcaacg aaagggctga tcccgcgtcg
     5161 gagttgtggc agtcggtgca tttggtcagc gatccgacgc gccaggggct tgtcaggctg
     5221 acgacgtcgt gattgttcct tggcgccgcg accggatgga acgacgggtt cgtcggctga
     5281 aactccagcc gggtgttggt ctgatggatg tcgcgagtga tcaccgcatt ctgcggccgt
     5341 gatgggttgt cggcgtgaca cttgaagcag acctcgtatt ccctcatcgc ttcgttcacc
     5401 ggcctgccca ggatgttcac tcccgagacg tatcgcattc tgttgttgat cggctccggt
     5461 gccgtggcac ccggccggct gatgaaatca taggcggccg catgcggatt atgacagtct
     5521 gcgcaagtga cgtgccggct catggtcagc ggatcctcga ccggatcatg gcagtcgaag
     5581 agcaggggcg cattgtgagc gctcggcttg cagatttcac tttcaatgtt ggtgctcgcg
     5641 acactgccat tatggcaatt gagacagttg tcttccaggt tcgcgaaacg gagcaatcgc
     5701 tcgcgacctc cggccgaatg aatcttgtga caattgccgc aggcgttttc cgccacggat
     5761 gcaaagggca gacgctctct catgtcgacg gcccggccgc ggatgacggc ccggctggtg
     5821 gcgtgactgg ccacgcccca gccgtccctg tcgtggcacg acaggcaaat ggccgatcgg
     5881 actttggtga tccgcaggaa gttgcccaga tggttgttgt gaggatcatg gcaggtggtg
     5941 cagtgcatct cgccgtgctt gccaagcggg agttcccgcg agatcacgcc gggatcatga
     6001 agctggtggt cgcggcccgc cagagcgcgg tcgtaacgaa aaccgatcgg gtggtcatcc
     6061 gacagatcca gcgacaggtt gccgcggcca agagccatgg tgcgccggct catcaccacg
     6121 atgtcatccg gccggccggc gagcaggccg atcgccagcg agccatcatg gcaactcagg
     6181 cacatcttcg acggaccgct gggctggtcg atgcgggcgt ctgtggttga actctcatag
     6241 atccggtaat gcgtgggagg catgtggcga ttccacagcg gacgctgccc ggtggcgttg
     6301 tgcggggtat ggcagaagat acagacctgc tcttcttcca ttgcgtgaat cctgcccgga
     6361 ccgccgacac tgaggtcatg cggagaccct acaacggtct gacggccggc ggcgctgctc
     6421 ctgggttgct cgcccgcaga cagaaggtcg ccgccgccga tcgcccagag agccagtaat
     6481 gtccatcgcc cgattctcat gatggcagca actcgaacac ttgcacacgg cggttatacg
     6541 tgtccgcgac ccagattcgt cctcgggcgt caattgtcaa acctgccgga agactgaact
     6601 cgcctattcc ctgcccttcg tgcccgagag acatcagcag ccgtccgtcg ggcgtgaacg
     6661 cttgcacatt ctcgaaatgg gcgtccacga cccagatatt ccctcgcggg tcgaatgcaa
     6721 cgcccttggg caacgaaaga tcacccgcgg cgtcgccttt cttgccgatt tgggcgatga
     6781 acgccccgtc cggtccgaga tactgaagtc gggaattcag cgagtcggcg acaaccagtg
     6841 cggggcccgg gccgcaggca aggtgtgagg gcccgttgaa ctggccgggg ccgttgccgt
     6901 gggttccaaa acgcagcacg cagactcctc gttcgtcaaa ggccagtagt gcgtgacctg
     6961 cggaatctga gacgtagcaa agaccgttct ctggacagaa cgcgatgccg gcgggccgct
     7021 ccagcgactc attccggatc cagcgcgagg ttgggcaccc cagaaccgcg atcgctccag
     7081 ccttcgcgtc ggccgtccag atggacgcgc ccacccacgc cacgccaacg ggacactgca
     7141 acctgtcgga ccgcccgatc gtcgtcagtc tttcatattt gcccgcctcg aggtcaagca
     7201 ggtgaacaca agccccgttg gtgtcggcga ccgctacgcg ccggcagtca ggatgcacgg
     7261 cgaccgcgtg cggcgtcaca aaaacctccg gtgacttcgg gccgtgcagc aattcgtccc
     7321 acctctcgct caggctcttc tttgcccgga cgctctggct gcccgcaagg cagccaagat
     7381 actgaacgcg ggcgacgtcc ggcggcttgg gccagaccac cggtggactg ggcggcgtca
     7441 acggcgatac cgcaacctga gagcagccgg agactgcaaa caggatgatc gccgctcctg
     7501 gtcttgcggt gtatccgtgc gccttgatca tggcaaatcc ccccggcgag gcagcagatg
     7561 agtgagattg cgccggacgt tgaggtatac cccgaaaccg ccgtcccggt tcccagcaat
     7621 cgacagcaaa tcgtactcca ctgtcaggtc cacggtcagc gctcctcgga catactgaaa
     7681 cccgcattcc aggtcgatgc cgtgggtcgt gccatcgacc gtgtcatcct cccatcggta
     7741 gcccaatccg ttcttcaacg caagcatctg attgatgcga aagcggtcct tgacatcaat
     7801 atccagccac cagacgcggc ggcgatccca acctccctcg aaccagtatc gcgacagctc
     7861 ccctgtcacg tcgagcgagt ggtcggccgt gcggataagc gatgcccggc cggtgaaatg
     7921 gcaggcatca tatggctcgg tgctgtcgtc atacagttca tactctgcac cggctgacca
     7981 gcgatcgcgg tcgaatcgcg acccgagacg atgccggtgg ctgtcgtgtc gtcgccaggc
     8041 ggttgcctcg ctcgtgttca cgtcttcgca gcggctctcc agtgtgtaat agggcgtgag
     8101 gccgaacttg aaggcgtgtt caaccaggaa ggagttgcga aactcgttca tacttgcctc
     8161 tgcggggatg atgtactggt agtcgaagta caccacctcg ttgtcctcga tgcggcccgt
     8221 gggaattcgc tcgactcgca cgagctgtcc gagatcggta accagataat cgctacccgc
     8281 aacatagtat ctcgtccggc gttcgttgtg cgccaggatc gatccgggca cgatcccccg
     8341 ctggttgagg taaacgggcc tggaaccccc aagaacgtgc ccttccgaga ggacgtagcg
     8401 tcgaccggcg tccccgtgga cctcggttcg ctcaaacgcg gaagcgaggt tgatggtcag
     8461 ctcgccggac gaggtcgggc ggacgtagct gacgtcaaac ccgccgccgt actccacaga
     8521 gtcgacgtcg tcgtccgccg cttcgtacag gccgaacgtg tccacagtga tccgcaggtc
     8581 ctcgcgcggt cgatacaggg cttccacgtc gaatttgtgt tgtgtgatat caatcgcgtc
     8641 ctgctgaaag cggtagaggt tgtaccggta caccgtcttc agcttgtcgg tatgcgccag
     8701 ggacaagcga ggcaggaact cgatctcgtc gcgggcccaa tcgccgtctt cttggccgta
     8761 acgcagcaac gtgtccagtc ggtgctgccc ggccgacccg aacgccagct cgtggctgag
     8821 acgaagttcg tcgcggcggc tgtcgtagtt gtagctgtcg ccttggtaat tgctctcctc
     8881 gcgatcgtga tcatattgaa cccgcagctt ctggtcctgg gagatggccc aggtatggtc
     8941 gaggtagaag cggctcactt caagctcctc gtcatccaga cggtcccggt ttccccgacg
     9001 tctcacgtcg ctccattcga ggccgagctc cgtcgtggtc ggccccgcga taatcaggga
     9061 ggatataccc gcgctggtat cgatctcgtg cagcgaaggc agaaaacgac gcggcagccg
     9121 atgatccgat cgcctggcgt aaacgctgaa ggaaacaggc ttggccttga ggacgtcgag
     9181 cgagacgtcg tattcgtgca ggaatccgtc gctttgattc tcctcgcggc tcacgccgtc
     9241 cgcctgctcg gtgaagcggg tctgcgatag cccgaattcc aacgttgccc ggtactcgat
     9301 aaggttggcg tcgtagatat gccccgccaa tccggccccg acgagttcgt agagccgaaa
     9361 atcacggttc tcatgcaggt agtcgctcgt ctcaccgcca taacggacgc gccgctgcgc
     9421 gtactcgccc tcgattccaa cgtacaggtc cgtcaggttg atgccgaata atcgccgctc
     9481 gccggataca ggggtctcgc ccgcggcagg ggcggtcgtc ggcggcgcct gacccagggc
     9541 aaccaaggcg aaaagcgggt ggacgatctg cagcaacgtc gcgatcgcct taataccggg
     9601 ttccactcgc atgtcgccgt tgccggttga tcagaagaga cgctcatcag agccttatgg
     9661 ccagaaatct gcgcggattg cgcgcatctt tctgaatcgc agtgcgcttt gtttgcgacc
     9721 cgcaaaaagg taatccgcct cagggacgga gcactcatgc cctcccgcac aagaacgtcc
     9781 cgcccccgtc tcgccgtagc cgcacccgcc aaggtccgtc atgactgtct tgggcgacga
     9841 tcatgcctgt gacacgccca gggcaagagc actgcggttg cgatcatgac agcgccggcg
     9901 aaccatgtca acggccatga ccgctttgcc ccggccgacc cccagccgcc ccaagatccg
     9961 ttcggatgct gaacgacggc tatggatgtt ccagagaacc aatgaaccga agcccactgc
    10021 accagctcgg tggattccgt cgcgttgtcc tgcataacgc gctgccggca cgttttgggt
    10081 gatcgccgga gcagggccgt ttgcacgaag agggatctgg cgagaactgc ttggctgcaa
    10141 acacgttgaa cggtagactg accgattctc gcaaagaaag ccggcgcggt gatccggtcg
    10201 aaaagccaag aataagcact gcgggatagc gtacaagatg atggacttca atgctgaaag
    10261 tgtgcgggac agtaggcgcc gtgcgcggtc gctgctgttc ggcttggcgg tttctgctct
    10321 tgcgctgggt caacgatgtg tccccatcca gccgcaggca tgcgttcccg agttggctga
    10381 tgatgaccac gtgttggcgg tcggccggcc ggcggtgacc gtgttcgagt acgccaactt
    10441 cgagtgttca cactgcgggc agtttgcgcg ggaaacctgg ccgaccctca gacaggagta
    10501 cgttgacact ggcaaagtcc gatggatcgc gcgacaccgg cccttccgca gcgcctacgc
    10561 tcctgtcggt gtggcggccc aggccagtga atgtgcggcc gatcagggag ctttctggga
    10621 ataccgcgag ctggtgtatg ccaaccaggc agacctgagc gtggcggccc tgaagtccta
    10681 cgcggtggaa ctggctctgg atacccaagc gttcgacgcg tgcctggacg gccaggtcaa
    10741 ggcggccgag attgacgccg aacgggctgc cgccgaggca ctgggggtgt gctgcacgcc
    10801 atacttcttc gtcgatggcc ggccctttgc cggattccgc accgtcgagg agttccggac
    10861 gatcctggac gccgccctcg ccgcagccgg ggccgattga tccggttcag cctggtcatg
    10921 ccgcggcgtt tggctttgtg tcgacctgac cgcttccggg ttcttcatca atcgctttca
    10981 ccgccgtagc cggcacctcg ccaatggtga agtccgccgc tcacggcaag acgcacatcg
    11041 tcctcgattc tgtgctcgac gttattactg tccggctgag acggcccgat tgtgtatcaa
    11101 atgcctgatt ctcaaggatt ccctcgccgc aggagggggt acacgat
//
LOCUS       DAOTPW010000186        11078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5548_length_11078_cov_5.348725, whole genome shotgun sequence.
ACCESSION   DAOTPW010000186 DAOTPW010000000
VERSION     DAOTPW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5548_length_11078_cov_5.348725"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1814)
                     /locus_tag="P5316_15645"
     CDS             complement(<1..1814)
                     /locus_tag="P5316_15645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR86442.1"
                     /translation="MADQDVHHSSAPDQSAPEGAVTESSPATAPAGVRPLLFEIAWEV
                     CCQTGGIYTVLRTKAPATARRWGDGYWLVGPYRPAAAAVEFEPEAASGIVGEALSLLR
                     ERGVVIHFGRWLITGRPQVLLVDLSSLAPHADQMKYFFWKDIGIGTPFGDREMGDMVV
                     FGYAVADLLSAIRDRSADRPMLAHFHEYQSAAALPILKHRQVALPTVFTTHATLVGRS
                     LSAANVDLYAHLPHSDGEAVANEHGIGSRFQLEKAATHCADVFTTVSGITALEAEQFL
                     RRKPDILLPNGLNVERFAAPYRFQELHRENKALIHEFTMGHFFPSYTFDLTKTLYFFT
                     AGRYEYRNKGFDLYIEALYRLNQRLKAHPNGVTVVAFIIAPANYRSPNVETLNRQAMF
                     NELRETCEAIKDEMGEHLFRTIAFGRMPTTEDLLDEFARVRLKRMMHAWRQGPPPTIV
                     THDLVDDSGDLILRHLRHRGLINLSEDPVKVIFHPEFITSTSPILGLEYDQFVRGCNM
                     GVFPSYYEPWGYTPMECVIRGIPAITSDYSGFGAYVMSHFPQHNQHGIFIARRRGVSI
                     DKTIDLITDWMHALTRMSTRQRIQLRNEVEAYAEHFDW"
     gene            complement(2032..3228)
                     /locus_tag="P5316_15650"
     CDS             complement(2032..3228)
                     /locus_tag="P5316_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR86443.1"
                     /translation="MSDFTKRPTGHPDTDAVHCGENRWRGTITTPIAYSSTFVFKDVN
                     EMLAFGKAEVPHYEYSRYGNPTRQAAERKLAALEKAERAVLFDCGMSAVCATILAYCS
                     QGQHLVITDDAYKQTLNFVRTVLPRFGIRASVVPMGDYDAMADAVCKDTVMIISESPT
                     NPYLNIADVERVVRIARDQKVLSVIDSTFATPFNQRPLEQGIDLVVQSTTKYLGGHND
                     ILGGSVAGRASVVEPIYQWQRVTGGVMDPMSCYLLIRGLKTFGLRMARLNSTAMTVAQ
                     WLEKQPQVKRVYYPGLPTHPHHDIARRQMRGFGAVVTFEVNGNLEQTLAFLDSLKLCL
                     MGPSLGGPETLITHPALNSYYNVSREERYQLGIIDELVRLSVGLEDPEDIIADLEQGL
                     AKCGAK"
     gene            complement(3310..4953)
                     /locus_tag="P5316_15655"
     CDS             complement(3310..4953)
                     /locus_tag="P5316_15655"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRR86444.1"
                     /translation="MRIALAQINPIVGDIVGNTRMIIDRISQARQAMASLVVFPELSV
                     TGYPPRDLLLKPRFIQANVEAARQIAEHCVGVSAIVGFVEQNKDPEGRRLRNAVAYCR
                     DCQIREVRHKSLLPTYDVFDEQRYFEPGPSVSLIEHEGVPLGVSICEDLWNDEQLIGR
                     RIYHQDPIGQLAAAGARLLINTSASPFWLDKHAFRRRLFSHQAGRHHLPIIFVNQVGG
                     NDELVFDGASMMIDARGEVVAQAKAFDEDLLVVDTGTPSGARIEPYPDGIASLHAALV
                     LGLRDYTAKCGFKRVVLGLSGGIDSAVTAALAADALGPDNVTGVAMPSRYSSDHSLAD
                     AEALVRNLGIRYKVIPIGALHETFEEQLKPHFEGRAPDVTEENIQARIRGVLLMALSN
                     KFGCLLLTTGNKSELAVGYCTLYGDMCGGLAVISDVPKTMIYRLAEYINNRAGRELIP
                     RSTMTKPPSAELRPNQTDQDSLPPYDLLDAILDMYIHRELSADEIVAAGYDARTVQKV
                     IRLVDTAEYKRKQAALGLKVTSRAFGFGRRMPIAAKFRY"
     gene            complement(5257..6351)
                     /locus_tag="P5316_15660"
     CDS             complement(5257..6351)
                     /locus_tag="P5316_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRR86445.1"
                     /translation="MWRRTNPDYPNPDDVRMTLGEHLEELRTRLIRAIIALVVGAVVC
                     FIYSDELLGFLCAPIFAILRKEGYPAELGFFSPAEAFMISLKVAIIVGFIITAPFSLT
                     QIWGFIAAGLYPHERKWVRRFAPASIALFFTGAGFLLVIAAPLLYSFLLTYQNKLPDR
                     TGLYPAFLLGTTERKPVEVDQADEGWPATAVSQPATTLPQGSESSWPPLTRIPALVAD
                     PVAPPEGVFWMNRTERQIRIRYGNQVYYLGSGGLTPVTQGPRLRPDIRISDNIIFVLQ
                     LSAAFGIAFQVPVIVAFLAAVGIAPVEQMARLRRYVWFGMSIASAIITPTTDVVSMLF
                     LLLPMVLLYEVGLWAGRLIEKRKPQATPAE"
     gene            complement(6579..7346)
                     /locus_tag="P5316_15665"
     CDS             complement(6579..7346)
                     /locus_tag="P5316_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86446.1"
                     /translation="MKTSLGAQCDEFYISSRLFFKLDLNLGRETVLHFFERIRRQYPL
                     LNRFRRREDDSLILEECDEQGEQTAPRHWIRLEPGALRFGCFSPPSTQDNRAFSAFLL
                     EQAPCHLTLSDLDVDHMEVVYGFDMEYSGNHDQLVAETLFADHPLAAFILGEDSAHTI
                     ECEPYFGIALSARCDAQAFLEIKSRTTSFEVRTGEYQPQMLSVYLTVRRYWGLNEAQD
                     LACAHAELLNLADDLAVRRVVPLVVNPLALAIAGRLS"
     gene            7765..8724
                     /locus_tag="P5316_15670"
     CDS             7765..8724
                     /locus_tag="P5316_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86447.1"
                     /translation="MTPIKSGSTLDQRVRTGIFLLMCVGMGVWFAYDGWVGYPAKNLA
                     WWADQKLPRRPGNLSTNPLATKANLSRIDVGTAPEQIRQLLGEPALDMPRTLTYAGTE
                     LTVEVRIDENSKVLDVQLTPVQPGKSPQNPGVLVTRLRAEMVKAGMTEDAVRSLLGDP
                     ASVGDRMMCYIGPAAYAEYHIVDGKVAAKPLILENEHRSENSILWQKGIAVCVGALAV
                     YALLKFWAAIRLRVLIDDEGMSYNGRRISYHSMTRLKTDQYKDKAWVDLEYRDGAAVR
                     LLRLDALVIQRFNEIMRVICDRKGFVLPSRSSGDDQSDGSGLS"
     gene            8760..10076
                     /locus_tag="P5316_15675"
     CDS             8760..10076
                     /locus_tag="P5316_15675"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HRR86448.1"
                     /translation="MSPSSLRSILVVVPALVGPLSAVGSEPRYPSRSDIPEWWRNTRA
                     FYCPWENSGAGASLMKFKAHRDEGFESFNDLDKVLDDARRLGTNVLYLVGYWEPDYEH
                     KAEYRPKLKWGGDTAFRQGIEKVHRLDGRVIVYLEAFIISRNTDLGRTKGPQWAMMDE
                     TGRYYPYDGTGDRFYLMYPGPGSGWTDYLVEVAARLARDFRIDGVHLDSYGLQWGWKN
                     HNPAHPEGRDPESFNRGAVELVRRVRDEMRKHVPDAVVILEGAEHTALLDVCDGAQIE
                     SLAVIHRKPWAAQGRYPVFTSSFSIEEMGRILDEGHNLAISPWWLMARPARRDEKTLL
                     AQTDKRSRFDQIEALHRYHNILLVNGRLPQPVADFDGLSQGILEHLNRNGWTSRFSHP
                     PLVEAAQRYIAAYNRDEGRLTRVPADLIRQMLLAASSRPAAASRPG"
     gene            complement(10090..>11078)
                     /locus_tag="P5316_15680"
     CDS             complement(10090..>11078)
                     /locus_tag="P5316_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRR86449.1"
                     /translation="MKDISLLIGEHVRCIVTQVDRTTCDLIVSRKKYLEKEAHERRRQ
                     ALGSLIEGDIRSGTVTNLTEYGAFIDLGGVDGLLHMTDISWGRVRDPREVLQVGQPIR
                     VRILKINHETGKVSLGLKQVKPNPWDGIETRYPKDRRLKAKVARMADFGAFLELEEGV
                     DALLPIGELSWSKHVNHPSDVVKIGDEIEVVVLKVDPEKKRISVSLKQTQENPWATVE
                     ARFPVNAKVKGKVVKVLDFGAFVEIAPGVEGLVHISELSEKRVKAVGDVVSEGQEVEV
                     RVVRLDVSGQRISLSMKPEQKTPLKSAEAAKLDKKKARKRPLRGGLSSHFEW"
BASE COUNT         2163 a         3349 c         3426 g         2140 t
ORIGIN      
        1 ttccagtcga aatgctcggc gtaagcctcg acctcattgc ggagttgaat gcgctgtctg
       61 gtcgacatcc gcgtcagggc gtgcatccag tcggtgatca ggtcgatggt cttatcgatg
      121 ctcacgccac gacgacgggc gatgaaaatg ccgtgctggt tgtgttgggg aaaatgactc
      181 atcacataag ccccgaaacc gctgtagtcg ctggtgatgg cgggtatccc tcgaatcacg
      241 cattccatcg gcgtgtaacc ccacggttcg tagtacgagg ggaacacgcc catgttgcag
      301 ccgcgaacaa actggtcgta ctccaacccc aggatcgggc tcgtcgaggt gatgaattcc
      361 gggtgaaaga tcactttgac gggatcctcg gatagattga tcaagcctcg gtgacgcagg
      421 tgtcgaagga tcagatcgcc gctgtcgtca accaggtcgt gggtgacgat cgtgggcggc
      481 gggccttggc gccacgcatg catcatgcgt ttgagccgga cgcgggcgaa ttcgtcgagc
      541 aggtcttcgg tggtgggcat tcgcccgaac gcgatcgtcc gaaacaagtg ttcgcccatt
      601 tcgtccttga tggcttcgca tgtttcgcga agctcgttga acatagcctg ccggttgagc
      661 gtttcgacgt tcggcgaacg atagttggcc ggagcaataa tgaaagcaac gaccgttacg
      721 ccgttggggt gcgccttgag tcgctgattc agacggtaca gggcctcaat atacagatcg
      781 aagcccttgt ttcggtattc gtagcgtcct gcagtgaaga agtaaagcgt ttttgtcagg
      841 tcaaacgtat agctggggaa aaaatgcccc atggtgaact cgtgaatgag ggccttgttc
      901 tcgcggtgca attcctgaaa ccggtacggc gcggcaaacc gctcgacgtt gagcccgttg
      961 ggcaagagga tgtcgggctt gcgccggagg aactgctcgg cctcaagggc ggtgataccc
     1021 gagacggtgg tgaagacatc agcacaatgg gtggccgctt tttcgagttg aaaccgcgag
     1081 ccgatgccat gctcgttggc gacggcctct ccgtcgctgt gcggcaagtg ggcgtagaga
     1141 tcgacgttgg cggcgctgag gctccggccg accagtgtgg catgcgtcgt aaagaccgtg
     1201 ggtagggcaa cctgccgatg tttcaggatc ggcaaggcgg ctgcgctctg gtattcatga
     1261 aagtgcgcga gcataggccg gtctgcgctg cgatcgcgaa tggccgagag cagatccgcc
     1321 accgcatatc cgaagacgac catgtcgccc atttcgcgat cgccgaacgg cgtgccgatg
     1381 ccgatatcct tccagaagaa gtacttcatc tggtcggcgt ggggagccag cgacgagagg
     1441 tccacgagta gaacctgggg ccggccggtg atcagccaac ggccaaagtg gatcaccacg
     1501 ccccgttccc tcaaaaggct cagagcctca ccgacgatgc ccgaggcggc ttccggctcg
     1561 aactcgactg cggcggcagc cggccgatag ggccccacca gccaataccc gtcaccccat
     1621 cgacgggcag tcgccggagc cttggtgcga aggacggtgt aaatcccccc agtctgacaa
     1681 cagacctccc aggctatttc gaaaagcaaa gggcgcaccc ccgcaggagc agtcgcgggc
     1741 gaggactcgg ttactgcccc ctctggtgcc gactgatcgg gcgcggacga gtgatggaca
     1801 tcctgatcgg ccatgcgata acctcggatg gaccccttcc ccgccgagac ggcggggaat
     1861 aatacgcgca gaagggccaa cagacaaatc tgctgtccac ctctcgacac agcctggcgc
     1921 gtggcatttc gtttgaaagg caaaagcaac gcgacttgcc ctgtgtgccg cgcctcgcag
     1981 atccggctca cccgttgcgt gctctggact ctgaaccccg gcccctccgc ctcactttgc
     2041 gccgcatttg gcgagccctt gctccaggtc ggcgataatg tcctcggggt cttccaggcc
     2101 gacggagagg cggaccagtt cgtcgatgat gccgagctgg taacgttcct cgcgagagac
     2161 gttgtagtag ctgttgaggg ccgggtgggt gatcagcgtc tccggtccgc cgaggctcgg
     2221 gcccatcagg catagcttga gcgaatccag gaaagcgaga gtttgctcga gattgccgtt
     2281 cacctcgaag gtcacgaccg cgccgaaacc ccgcatttga cgccgggcga tgtcgtgatg
     2341 ggggtgagtg ggcaggcccg gatagtaaac ccgcttgacc tgcggctgct tctcgagcca
     2401 ctgtgcgacc gtcatggcag tgctgttcaa ccgcgccatg cgcagcccaa aggtcttcag
     2461 cccacgaatc aacaggtaac agctcatcgg gtccatgacc ccgccggtaa cccgctgcca
     2521 ctggtagatc ggctcaacca cggaggccct cccggcaacc gagcccccca gaatatcatt
     2581 atgaccgccg agatacttgg ttgtactctg aacgacgaga tcgatgccct gctcgagcgg
     2641 ccgctggttg aacggcgtgg cgaaggtgct gtcgataacg ctgagaacct tttggtcgcg
     2701 cgcgatccgg acgacccgtt cgacgtcggc gatgttgagg tacgggttgg tgggcgattc
     2761 ggaaataatc atgacggtgt ctttgcacac cgcgtcggcc atggcgtcgt agtcgcccat
     2821 cggcacgacg ctggcccgaa tgccgaatcg cggcaaaacc gtccgcacga aattgagcgt
     2881 ctgcttgtag gcgtcgtcgg tgatgaccaa gtgttgtccc tggctgcaat aggccaggat
     2941 ggtggcgcac acggcgctca tcccgcagtc aaaaaggacc gctcgctccg ccttttccaa
     3001 ggcggccagc ttgcgttccg ccgcctggcg ggtcgggttg ccgtatcggc tgtattcgta
     3061 atgcggcact tcggccttgc cgaaagccag catttcatta acgtccttga aaacgaaggt
     3121 gctgctgtag gcgatcggcg tggtgattgt gccgcgccag cggttctctc cgcaatgtac
     3181 cgcatccgtg tccggatgcc cggtcgggcg cttggtgaaa tcgctcatgg tgtctcccga
     3241 atcacgtctt acatctcagt catcaaggta tattctattg agggtggcaa gttgggtcca
     3301 cgcgtggcgt cagtagcgga acttcgcggc gatcggcatc cgccgaccga agccgaaggc
     3361 tcgggaggtg actttcaggc ccagtgcggc ctgcttacgc ttgtactcgg ccgtgtcgac
     3421 caggcgaata accttctgca cggtccgggc atcgtatccg gcggccacga tttcgtcggc
     3481 gcttaattcc cggtggatgt acatgtccag aatcgcgtcg agcaggtcgt aaggcggcaa
     3541 cgaatcctga tccgtctggt tgggccgcag ctcagccgac ggcggtttgg tcatggtgct
     3601 gcgcggtatc agctcgcgtc cggctctgtt gttgatgtac tcggcgagcc tgtaaatcat
     3661 ggtcttgggt acgtcgctga tcaccgccag cccgccgcac atgtcgccgt acagggtgca
     3721 gtagcccacg gccagctcgc tcttattacc cgtcgtcagc aacagacagc cgaacttgtt
     3781 cgagagagcc atcagaagca cgccgcggat gcgggcttga atgttctctt ccgtgacgtc
     3841 cggcgcacgg ccctcgaaat gcggcttgag ctgctcctca aaggtctcgt gcagggcccc
     3901 gatcggtatg accttgtagc gaataccaag gttgcgaacc agggcttccg cgtcggcaag
     3961 gctgtggtcg ctgctgtaac gggagggcat ggcgacgccg gtgacgttgt ccggcccaag
     4021 ggcatcagcg gccagggcgg cggtcacggc cgagtcgatg ccgccggaga gccccagcac
     4081 gacacgcttg aaaccgcact tagccgtgta gtcccgcaag cccagaacca gtgcggcgtg
     4141 caacgaagcg attccatcgg ggtagggttc aatccgagca cccgagggcg tgccggtgtc
     4201 gacgaccaga agatcttcgt cgaatgcctt cgcctgggcg actacttctc cccgggcatc
     4261 gatcatcatt gacgccccgt cgaataccag ctcgtcgttt ccgccgacct ggttgacgaa
     4321 aatgatgggc aggtggtgcc tgcctgcctg gtggctgaag agccgccgtc ggaaggcgtg
     4381 cttgtcgagc cagaaaggcg atgcgctcgt gttgatcagc aacctggcgc cggctgccgc
     4441 caactgcccg atggggtcct gatgatagat ccgccgaccg atcagttgtt cgtcgttcca
     4501 aaggtcctcg cagatgctca ccccgagcgg gacgccctcg tgttcgatca gagacacgga
     4561 cgggcccggt tcgaaataac gttgctcatc aaagacgtcg taggtcggca gcagcgactt
     4621 atgccgaacc tccctgatct ggcaatcgcg acagtatgcc accgcgttgc ggagccgccg
     4681 gccttccgga tccttgttct gctcgacgaa tccgacgatc gccgaaacgc ccacacagtg
     4741 ctcggcgatc tgccgggcgg cctcgacgtt tgcctgtatg aatcgcggct tgagcaacag
     4801 gtctcgcggc ggataacccg tcacggacaa ctccggaaaa acgaccagcg aggccatggc
     4861 ctgccgggct tgcgagatcc ggtcgatgat catgcgtgtg ttgccgacaa tgtcgccgac
     4921 aatcgggttg atctgtgcga gggcgattct catggggtca aaaacttcca ggtcgaagcg
     4981 ctgcggcgct tcggccgccc ggcaacaggt cttgcgacgg aacaacacgg aacccgcgga
     5041 gcgtcgccgc gcggattgcc cagcatctta gtcgtatcgg ccggtgtcgg ccagttgggg
     5101 cgagtccgcg atgagccggt cccgaagaat agctgatcag ccggcgtctt acgcagtgtt
     5161 tgcggccggc agtctctgtc ggccggtcaa acgggaccgg cgatacttgt tcccgactct
     5221 ggtgacgggg tttgccccac agcgggaagg tgtggcttac tctgccggcg ttgcctgcgg
     5281 tttgcgcttc tcgataagtc gtcctgccca caggccgact tcgtagagca gcaccatcgg
     5341 cagaaggagg aagagcatgc tgaccacgtc cgtggtgggt gtgatgatcg ccgaggcgat
     5401 cgacatgccg aaccacacgt agcgacgcag gcgggccatc tgctccacag gggcaatccc
     5461 gacggcggcc agaaaggcga cgatcaccgg cacctggaaa gcaatcccga aagccgcgga
     5521 aagctgcaac acgaagatga tgttatcgct gatccggatg tcgggtctga gccgtggccc
     5581 ctgcgtgacc ggcgtgaggc cgccgctgcc cagatagtag acctggttgc cgtagcggat
     5641 gcggatctga cgctccgttc tgttcatcca gaacacgcct tcaggggggg ccacggggtc
     5701 cgcgacgaga gcaggaatgc gtgtcagcgg aggccaagat gactcggagc cctgcggcaa
     5761 cgtcgttgcg ggctgagaaa cggccgttgc aggccaacct tcgtcggcct gatcgacctc
     5821 gaccggcttg cgttccgtgg tgccgagaag gaaggcagga tacaagcccg ttcgatcagg
     5881 cagcttgttc tggtaggtca gcagaaaact gtagagaagc ggcgcggcga tcaccaggag
     5941 gaaacccgcg ccggtaaaaa acagggcgat cgacgccgga gcaaaacgtc ggacccactt
     6001 gcgctcatgc ggatacaggc cggcggcgat gaaaccccag atctgcgtca ggctgaacgg
     6061 cgcggtgatg atgaagccga cgatgatcgc gaccttgagc gagatcatga aggcctccgc
     6121 agggctgaaa aagcccagct cggccggata gccttccttg cgaaggatgg cgaaaatcgg
     6181 agcgcacaga aagcccagca actcgtcact gtagatgaag cagaccaccg ccccgaccac
     6241 cagtgcgatg atcgcccgga tcagccgcgt gcgcagttcc tccagatgct ctcccaacgt
     6301 catccgaacg tcgtcgggat tcgggtaatc cggattggtt ctgcgccaca tggccatgta
     6361 tcaggctctg ctctgccggc cgggcagcgg ttatctgttc gcgggcgcct cagaagccgg
     6421 agaccaaacg gcatccccaa tcttcgagat aacaaccacg ccaacggtcg acgtcgcggt
     6481 gcgattctag ggacgacctt taaggtggtc aaatgatgac tcgtttgcca gcccgttgcg
     6541 gcgtcggtcg agcggaaaca tccggcggca tgccgttctc acgagaggcg cccggcaatt
     6601 gccagagcca gcgggttgac taccaacggc acaacccgcc gaacggccag gtcgtcagcc
     6661 aggttcagaa gctcagcatg ggcgcacgca agatcctggg cctcgttgag cccccaatat
     6721 cgccggacgg tcagatagac gctgagcatt tggggctggt attcgccagt gcggacttcg
     6781 aaggacgtgg tacggctctt gatttccaaa aacgcctgtg cgtcgcagcg cgcggaaagt
     6841 gcgataccga aatacggctc gcactcgatg gtgtgggcgc tgtcctctcc cagtatgaaa
     6901 gcggccagcg gatggtcggc aaacagggtt tctgccacaa gctggtcatg gttgccgctg
     6961 tactccatat caaaaccata aaccacctcc atgtggtcga cgtccagatc gctgagcgtc
     7021 aaatggcagg gcgcctgttc aagcaggaag gccgagaagg cgcgattgtc ctgtgtcgaa
     7081 ggcggcgaaa agcacccgaa ccggagggcc ccgggctcaa gtcgaatcca gtgacgcggc
     7141 gcggtctgct cgccctgttc gtcacattcc tcgaggatca gcgagtcgtc ctctcggcga
     7201 cgaaaccgat tcaacaaggg atactgccga cggatccgct caaaaaaatg caggaccgtc
     7261 tcgcgtccga ggttcaggtc cagcttgaag aaaagtcggc tgcttatgta aaactcgtcg
     7321 cactgggctc cgaggcttgt tttcattgtc gtctctccat cagacggccg cgctcccggg
     7381 cgcccggccg gtccgactgg cttaccaaga cccgattatc gcaattcggc cgatcaagta
     7441 caagggggca aaccatgacg gcgagcgcca ggcccatgac attttgggcg gttcatcccg
     7501 aaccccgccc gcgagaaaga accaggctcc agagcgacca gaccccaaag gtcattccct
     7561 tacggtcggc cggtcggctc ggcgagcttc ggttgcagtt ctgaaagaga ccctgaagaa
     7621 accgtggtgt ttccccgcca aatgcggacc gacgccccgg tcgcgccttg catttcggct
     7681 ggaatcctgg aatcatatgc agcctgcggg attctcggcg gcgttggcag ggtcggttgg
     7741 ttcagcggta acaaggtgcg attcatgaca ccgatcaaat ccggatccac gctcgatcag
     7801 cgggtgcgca ccggcatttt tctcctgatg tgcgtgggca tgggcgtctg gttcgcctac
     7861 gacggctggg tgggataccc ggccaagaac ctcgcctggt gggccgacca gaagctgcct
     7921 cgacgcccgg gaaacctctc tacgaacccc ctcgcgacaa aagcgaacct cagccggatc
     7981 gacgtcggga ccgccccgga acagatccgg caattgcttg gggagcccgc gctggacatg
     8041 ccgcggacgc tgacctacgc cggcacggaa ctgactgtcg aggttagaat cgacgagaac
     8101 agcaaggtgc tcgacgttca actgacgccc gtgcagccgg gcaagtcccc ccagaaccct
     8161 ggcgttctgg tcactcgcct ccgggcggag atggtcaagg cgggaatgac ggaggacgcc
     8221 gtccgttcgc tgctcggcga tcccgcatcg gtcggcgatc gtatgatgtg ctacatcggg
     8281 ccggccgcct atgcggagta tcacatcgtc gacgggaagg tggccgccaa gccccttatt
     8341 ctcgaaaacg agcatcgttc agaaaactcg attctctggc agaagggaat cgccgtctgc
     8401 gtgggggcgc ttgccgtcta tgccctgctg aagttctggg ctgcgattcg tttgcgcgtg
     8461 ctgatagatg acgagggtat gtcttacaac ggccggcgca tttcgtacca ctcgatgacc
     8521 cggctgaaga ccgaccagta caaggacaag gcctgggtgg acctggaata ccgcgacggc
     8581 gcggcggtgc gcttgctgag gttggatgcg ctggtgatcc agcggttcaa cgaaatcatg
     8641 cgcgtgatct gtgatcgcaa gggcttcgtc ctgccgagtc gttcatccgg cgacgatcag
     8701 tccgacggca gcggtctgtc atgaagccct ctgggcggtt ctgtcgtcga ttccctcgct
     8761 tgagtccaag ctccctgagg agtattctcg tcgtggtgcc ggccctggtc ggcccccttt
     8821 cagcggtcgg ctctgaaccg cgctacccct cccgctccga tattcccgaa tggtggcgca
     8881 acacccgtgc cttctactgt ccgtgggaaa actccggggc cggcgccagc ctcatgaagt
     8941 tcaaggccca ccgagacgag ggcttcgagt cgttcaacga cctcgataag gtgctcgacg
     9001 atgctcgccg gctcgggacg aacgttctgt acctggtcgg ctactgggag ccggattacg
     9061 aacacaaggc ggagtaccgg ccgaaactca agtggggagg agacaccgct tttcgccaag
     9121 gcatcgagaa ggtgcatcgc cttgacgggc gggtcatcgt ctatctcgaa gcattcatca
     9181 tctcacgcaa caccgacctt ggtcgcacaa aagggccgca atgggctatg atggatgaga
     9241 cgggcagata ctatccgtac gacgggaccg gtgaccggtt ttacctgatg taccccggtc
     9301 cgggatcggg ttggacggac tacctcgtcg aggtcgccgc ccggctcgcc cgtgatttcc
     9361 gaatcgatgg cgttcacctg gacagctacg ggcttcaatg gggatggaaa aaccacaatc
     9421 cggcccatcc ggaaggccgg gatcccgagt cattcaaccg cggagcggtg gaactggtcc
     9481 ggcgggtgcg tgatgagatg cgcaagcacg ttccggatgc ggtggtgatt ctggaaggcg
     9541 ccgagcatac cgccctgttg gatgtgtgcg acggcgcgca gatcgaaagc ctggcggtga
     9601 tccatcgcaa gccgtgggcc gcccaagggc ggtatccggt tttcacctcg tctttctcga
     9661 tcgaggaaat ggggcgtatc ctggacgaag gccacaacct ggccatctca ccctggtggt
     9721 tgatggcccg gcccgcaaga cgcgacgaga agaccctgtt ggcacaaacg gacaaaaggt
     9781 cgcggtttga ccagatcgaa gctcttcatc gttaccacaa catcttgctg gtcaatggcc
     9841 gactcccaca gccggtggct gattttgatg gattatcgca agggattctc gaacacctca
     9901 accgcaacgg ctggacaagc cgcttcagcc accctccgct cgtcgaagcg gctcaacgat
     9961 atatcgcggc ctacaaccgc gacgagggtc ggctcacccg ggtacctgcc gatctgatca
    10021 ggcaaatgct gctcgctgcg tccagccgcc ccgcagccgc cagccggccc gggtaaggcc
    10081 tccggtcgct caccactcga agtgcgacga taagccccct cgcagaggcc gtttgcgcgc
    10141 cttcttcttg tcgagcttgg ctgcctcggc gctcttgaga ggtgtcttct gctccggttt
    10201 catcgagagc gagattcgct gcccggatac atccagtctg acaacgcgaa cctcgacctc
    10261 ctgaccttcg cttaccacgt cgccgaccgc cttgacccgc ttctcagaca actcggaaat
    10321 gtgaacgaga ccctcgacgc ctggggcaat ctcgacaaag gccccgaagt ccagaacctt
    10381 cacgaccttg cccttgacct tcgcgttgac ggggaatctg gcctccaccg tggcccaggg
    10441 attctcctgg gtctgcttga gactcacgga gatgcgcttc ttctccggat caaccttgag
    10501 gacgaccact tcgatctcat cgccgatctt caccacgtct gacggatgat tgacgtgctt
    10561 cgaccagctc aactcgccga tcggcaacag ggcatcgact ccttcctcaa gctccaggaa
    10621 tgccccgaag tcggccatac gcgcgacctt ggctttcaat cgccgatcct tggggtagcg
    10681 agtctcgatt ccgtcccagg ggttcggttt gacttgtttg agacccagcg aaaccttgcc
    10741 ggtttcgtgg ttgatcttga gaatccgtac gcggatgggc tggccgacct ggagcacttc
    10801 gcgagggtcg cgtactcgcc cccagcttat gtccgtcatg tggagcaggc cgtcgacccc
    10861 acccagatct atgaacgctc cgtactccgt caggttggtg accgttccac tgcggatatc
    10921 accctcgatc aggctcccga gagcctggcg acgcctctcg tgagcctcct tctccaggta
    10981 tttctttcgg ctgacgatca ggtcacaggt tgttcggtcg acttgggtca cgatgcagcg
    11041 aacatgctcg ccgatcagca gcgagatgtc tttcatcc
//
LOCUS       DAOTPW010000187        11059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5563_length_11059_cov_5.120774, whole genome shotgun sequence.
ACCESSION   DAOTPW010000187 DAOTPW010000000
VERSION     DAOTPW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5563_length_11059_cov_5.120774"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="P5316_15685"
     CDS             complement(<1..760)
                     /locus_tag="P5316_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRR86450.1"
                     /translation="MGGLFAAVSRDDCTHDLFYGTDYHSHLGTKRGGLAMANGSGYTR
                     FIHDITNAQFRSKFEDDIRRMHGHRGIGVISDYEDQPLIIGSHLGAYAIVTVGAVRNA
                     DTLAAKAFGERRIHFSEMSGGEINPTELVATLINQESSFVDGIRNVQESVDGSCSLLL
                     LTREGIYAARDKYGRTPLVIGRKPGAWAVTLETTALPNLGYEVDRYLGPGEIILVTQD
                     GVEQKSPPGDQMQICSFLWVYYGYPASSYENINVE"
     gene            1325..1888
                     /locus_tag="P5316_15690"
     CDS             1325..1888
                     /locus_tag="P5316_15690"
                     /inference="COORDINATES: protein motif:HMM:NF013312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86451.1"
                     /translation="MATTQLRRGMLIRHQNHVYEVADFQERHTGKQKPTVHVALRDVR
                     DGRPVDRTLNDLEPINEVARAYRQMQYLYRRDETRVFMDCETFEEYELSEAQIRGGSE
                     FLVEGQEYRVSFIDGSPAFLDLPDTIAVKIAMTAPPEHSVGVASNITKEATLENGLQI
                     RVPLFLKTGDVIRVDTRTKTYAGKEHG"
     gene            complement(1915..2760)
                     /locus_tag="P5316_15695"
     CDS             complement(1915..2760)
                     /locus_tag="P5316_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86452.1"
                     /translation="MDDLGFTGSTSRSTDSLPAATDIELFCPHCDYNLRGLCQDRCPE
                     CGNHFNRERLIRWSTEPNLPLGFTRSDNPDQHGRVLTNSMTRPARLARELPPMPCKAG
                     ATTYGWAMRGAGVFVIFMAAAIMSYDADAAGYALLFSVGPVVGTLFCEAIIALLLARL
                     VEPLAVLRGAEYRFWRTLCACFSTYFPISCAVVLFLPRIFIEIADSSATLDDFVIFCL
                     QLAMMTIPALMILWWWLALAKAITARGRASSGRTAAVWLIPVVGLVSVGVGFALDYLL
                     AMILL"
     gene            complement(3080..4558)
                     /locus_tag="P5316_15700"
     CDS             complement(3080..4558)
                     /locus_tag="P5316_15700"
                     /inference="COORDINATES: protein motif:HMM:NF025658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HRR86453.1"
                     /translation="MKHLFLLWLLLLGEVLVSGARAEGPLPRPATIFEDPADGGRNGE
                     VQRMTDARIHDMVDDLLDWDSCTVAYRNLLAEGKDCVPHLLKALKDPRFHQGRRKSRG
                     WQCPVNEIVELLIKFEPEIAARELKTLLNSPKGAIRRKAAFCLGRMATDECAEPVKNV
                     LTGKDHELQVHATRGIAEAVSENRLSKAFGAAVFEPLVAILSADDEVSHEVPKCLMKI
                     DATRAAGVLTNDKFLDPSSRLLEPVLEALEQGKVAVPTDKIIHLLGKLRAQAARYPNS
                     REYALGLLILAHNRCPQTESLISQTLVWGDKETRLMAMRARCVWEGIPDPDAFVEKLN
                     EERRLSELSDPQKKLVYVMNLEHHVGDDGFTDYFTCTCGRNAALAVSALREIGALRTA
                     RLLEKAMQEFGDKGPSRNTDVRKQQVSEMTDEQLENLDKLSERFRKDEDERRLLVLQF
                     ALKHKDHFRPAMTSKTATSGPAAKTPAAPATSQAGAESAPSG"
     gene            complement(4617..5477)
                     /gene="folP"
                     /locus_tag="P5316_15705"
     CDS             complement(4617..5477)
                     /gene="folP"
                     /locus_tag="P5316_15705"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRR86454.1"
                     /translation="MMSQQVLHIKGRPLVRGAGPFVFGVLNVTPDSFSDGGRYARASE
                     AIEAGVRMAEEGADVIDIGGESTRPGSQPVPADEQIRRTRPVIAELARQFGSRGPSIS
                     VDTRLAEVARAAVDAGATIINDITALRDDQALAPLAASTGVGVILMHMQGTPATMQQC
                     PTYRNVVAEIRQFLAERIVAATTAGIPLERLVVDPGIGFGKTTTHNLEILRRAGEFRD
                     LGVPVLIGPSRKRFIGEVLGIPEPIKRLYGTLAAVAACVLAGVECVRVHDVAACRQVA
                     DLCAAIRSAG"
     gene            5754..6899
                     /gene="recA"
                     /locus_tag="P5316_15710"
     CDS             5754..6899
                     /gene="recA"
                     /locus_tag="P5316_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019606868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRR86455.1"
                     /translation="MTQSTTTATGGNREQALARALQQIEKSFGKGAIMKLDQHETTVH
                     DGISTGTLSLDLALGGYGIPRGRVVELFGPESCGKTTLALHVAASAQRAGGVAAVIDA
                     EHALDPTWAKRCGVKLEELLVSQPDTGEQAMEIVEMLVRSNAVDCIVVDSVAALVPKA
                     ELEGEIGQSHVGLQARLMSQSLRKLTGIISKSKCVVIFINQLREKIGVMFGNPETTPG
                     GRALKFYSSIRLDMRKITTLKEGETAIGNRVRAKVVKNKVAAPFQSAEFDIMFNSGIS
                     SEGDLIDLAIAEGLIEKTVAWISYGQVRLGQGRENAKQFIQENPDLANEIREKILARR
                     RPAATPASGDGKVPSETSKATAVTKEDPAAKDRGKAKPKFPPPKGKR"
     gene            7526..9430
                     /locus_tag="P5316_15715"
     CDS             7526..9430
                     /locus_tag="P5316_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86456.1"
                     /translation="MSSMFGLVVSARHVLVSAVTHVLLAGLLLSATPRAEAQDAAARL
                     TQTSAISKLPEIQRLGDDTTAYVFRMGWEELKPQLAGTQALEAIEDPQIQAFFKAFKT
                     AGERSHPASPFFQFLLSALHDRLVMVTRPIPGGETQSSEEEEEQGKTAKPGTQNFAII
                     TPGPQGKAAFGELYGKAIGALKEAAEAELEKEKIGSLTFDVLEKTGTGLHTAWNNEQC
                     YWAVGAGAARWAAESSAPARSLDRSELFQTSIGPLFKGQQRQPIAMYYYDLRPWWRKV
                     DGMDHQGMWKQVSWHSLDAVAGATFIEKDHYRNRHYWRIGPERYGLFKDSRTARINEA
                     WLKRVPADSSGFTTGVYDACSLMAFLPTIFMHLFGADEKSIMQAPMFEMPFQPVLKEL
                     GPRYLIYRMPGRYGSFPIADVFPLSNAVVISEVRDAAAFKEALQTFAIGPVQPHTSTV
                     LGRTVISINVMYFTIYFAVFDNEVLITMNPQLLKDAIEAAERPGPSVIETAAYKEAVR
                     GVSPDACFLLYMPPGGFSRGIYDHYIPMLQQTIALAGAFRGFDSKQKPASTLDPIVFP
                     RGSDIARHARQATILSAVDDGEGVLFDGTAPILTTPYLGLRACVEQVASDRGRVSTFD
                     AGGCAVHWRT"
     gene            9576..10232
                     /gene="queC"
                     /locus_tag="P5316_15720"
     CDS             9576..10232
                     /gene="queC"
                     /locus_tag="P5316_15720"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRR86457.1"
                     /translation="MRIVLIHSGGLDSTVLLYKLRAEGHEVRCLGVDYGQRHRRELDA
                     GQAICRDVGVEYRVADLRGLRSLLAGSALTDSIPVPSGHYTDETMKATIVPNRNMIML
                     SVAIGWAVSLRYDAVAYAAHAGDHTIYPDCRPVFVEAVRSVARLCDWRPIELLAPFVG
                     KSKADIVRIGAELGVPFERTWSCYRGGELHCGTCGTCVERREAFQKAGIADRTVYRGQ
                     "
     gene            10269..10661
                     /gene="queF"
                     /locus_tag="P5316_15725"
     CDS             10269..10661
                     /gene="queF"
                     /locus_tag="P5316_15725"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRR86458.1"
                     /translation="MDGLHHLGKPSNQPTEKVDLIEWSGQPMTVRLECSEFTSLCPVT
                     GQPDFGQIIIEYVPGRFLVETKSLKLYLWKYRSEPGFNESLVDRIASDLFEQIRPLWL
                     RVRGQFNPRGGIRVTATAERGDNRHRPS"
     gene            10658..>11059
                     /locus_tag="P5316_15730"
     CDS             10658..>11059
                     /locus_tag="P5316_15730"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRR86459.1"
                     /translation="MTTIVRTIQFSAGHRLRQHEGKCAHVHGHNYTVEFHATAEQLDS
                     LGRVMDFSVLRDRLGGWIDQHWDHGFICSKDDREVLDAMALVSGQKLFVMEVNPTAEN
                     LARYLLDVVGPEQLAGTGVRLVRVVLAETENC"
BASE COUNT         2152 a         3210 c         3583 g         2114 t
ORIGIN      
        1 cctcgacgtt gatgttttcg tagctcgacg ccggatagcc gtagtagacc cacaagaacg
       61 agcaaatctg catttggtca ccgggcgggc tcttttgttc gacgccgtcc tgtgtcacca
      121 ggatgatctc gcccgggccc agataccggt cgacttcata accgaggttg ggcagggccg
      181 tggtctcaag ggtcaccgcc caagctccgg gtttgcggcc gatcaccagg ggggttcggc
      241 catacttgtc gcgggcggca tagatacctt ccctggtcag cagcaacagc gaacatgagc
      301 cgtcgaccga ctcctgcacg tttcgaatgc catcgacgaa ggagctttcc tgattgatca
      361 gcgtggctac cagttccgtc gggttgattt cgccgccgct catttccgaa aagtgaattc
      421 ggcgctcgcc aaaggccttg gcggcgaggg tgtctgcgtt acggaccgcg ccgacggtga
      481 cgatggcgta ggcaccgagg tggctgccga tgatcagcgg ctgatcctcg taatcgctga
      541 tgacgccgat gccccgatgg ccgtgcatgc ggcggatgtc gtcctcaaac ttggatcgga
      601 actgggcatt ggtgatatcg tgaatgaagc gggtgtagcc gctgccgttg gccattgcga
      661 gcccgcctcg cttggtgccc agatgggagt ggtagtcggt cccatagaaa aggtcgtgtg
      721 tgcaatcgtc acgggagact gcggcaaaca aaccacccat gaatggctcc tcctgcacgg
      781 cgccgctgtc agcggcccat cggggttcgg cgttcagccc atggtgcccc ccggccgtcg
      841 ccggggccta cgggaaatgg agtcctgtat gcttgcggcg gcccgcgcca gcggcccgca
      901 ttcagctatt ccgcaaggca ttaacaattg tgacgcaagc gctcagccgc gcaaggctga
      961 ccggatggca acgtatcttg ccgccggggg ttgtgcaagc gtgaaagaag ccggtctttt
     1021 ccgccggttg tgagttccag gcggtctcgt gcgataaatc gttgattctg gggggattgc
     1081 ggcggcgggg gaatgcgaag gtttctttcc ggggcctatc tttgagaacg gcgactgcaa
     1141 gggggcagcc tggttgtggc ccgctgccgt tgggtgccct gccgttggag atctccggct
     1201 gctgaaagag cctcgcggct cagccggcca tcgccgcacg aacgaagtgg ggcatgcaga
     1261 ctctcgagcg gcatgctaca atgattgggt aaccatttcg cctgtttcta caggaggcac
     1321 gagcatggca acgacccaat tgcgacgcgg catgttgatc cggcaccaga accatgttta
     1381 cgaggtcgcc gatttccagg agcgacatac cggcaagcag aagccgaccg tccacgtggc
     1441 cctgcgtgac gtgcgcgacg gtcggccggt ggaccgcacc ctcaacgacc ttgaaccgat
     1501 caacgaggta gcccgtgcct atcggcagat gcagtatctg tatcgtaggg atgagacgcg
     1561 ggtattcatg gattgcgaaa cgtttgagga atatgaattg agcgaggcac agattcgcgg
     1621 gggcagcgaa ttcctggtcg agggccagga ataccgcgtc tcgtttatcg acggttcacc
     1681 ggccttcctc gacctgcccg acaccatcgc ggtcaagatc gccatgaccg cgccgcccga
     1741 acactccgtc ggcgtcgcgt ccaacattac caaggaagcg accctggaaa acggcctgca
     1801 aatccgcgtg ccgttgtttc tcaagaccgg cgacgtcatc cgcgtcgaca cgcgaaccaa
     1861 gacctacgcg ggcaaggagc acggttagaa gaacgcgggc cgtcgctgcc ctatctacaa
     1921 cagaatcatc gccaggaggt agtccagggc aaacccgaca cccaccgata ccaaacccac
     1981 aaccggaatg agccagacgg cggcggtcct gccgctggat gcccgcccgc gagcggtgat
     2041 cgccttggcc agtgccagcc accaccacag gatcatcaaa gccggaatgg tcatcatcgc
     2101 cagttgcagg cagaatatga caaaatcgtc gagagtcgcc gaggaatcgg cgatttcgat
     2161 gaaaatgcgc ggcaagaaga gcacgaccgc gcatgagatg ggaaagtaag tggaaaagca
     2221 ggcacaaagg gtgcgccaga accgatattc ggcccccctc agcaccgcca agggttcgac
     2281 gaggcgagcc agcagcaggg cgatgatcgc ttcacagaaa agcgtgccga cgacgggacc
     2341 gaccgaaaac agaagagcat atcccgcggc atccgcatcg tagctcatga tcgcagcggc
     2401 catgaagatg acgaacacgc cggcgcctcg catcgcccac ccgtacgtcg ttgcgccggc
     2461 tttgcaaggc atgggtggta gttccctggc cagccgggcc gggcgggtca tgctgttcgt
     2521 caacacgcgg ccatgctggt cggggttgtc tgagcgcgtg aagcccagcg gcaagtttgg
     2581 ttcagtgctc cagcgaatga gtctttcacg gttgaagtgg ttgccgcact ccgggcagcg
     2641 gtcttggcag aggccgcgca ggttgtagtc gcaatgcggg cagaacagct cgatgtcggt
     2701 agccgcgggc aggctatccg tggaccgcga tgtcgatcct gtgaaaccaa gatcgtccat
     2761 ggacggcgac ctccgagaag aatcgtcagg tgattgacct gacttttcaa gtctaccgaa
     2821 tacgaaggcc cgtcgcaccg attcccctta tgattgttcg acgaaccggg catgaattgc
     2881 cgtcaccgtg ggacgggcat gccccaattg tcgccgtacc ggcaagggat tgccgttcgt
     2941 gcggagaaga gataggccag acaaccgtat tcatgcctga gaagctgcgc gcttgcccga
     3001 ttgctcagcc ggcggcacag ggaatccgcg ggacgcgccg gtgaagcgct gcttgccaag
     3061 gcttatggcg gcgatcgggt catccggagg gcgctgactc tgcgcctgcc tgggaagttg
     3121 cgggggctgc cggggttttg gcggccgggc ctgaggttgc ggtcttcgaa gtcatcgccg
     3181 gccggaaatg gtccttgtgt ttgagggcga actgcagcac cagaaggcgc ctttcgtcct
     3241 cgtccttacg aaacctctcg ctcagcttgt cgaggttttc gagctgctcg tcggtcatct
     3301 cggatacctg ctgtttgcgt acatcggtgt tgcgcgaagg tcccttgtcg ccgaattcct
     3361 gcattgcttt ttccaggagc cgagccgtcc gcagtgctcc gatttcccgc aaggccgaga
     3421 ccgccaacgc ggcgttgcga ccacaggtac acgtgaagta gtcggtgaaa ccgtcgtctc
     3481 cgacgtggtg ttcgaggttc atcacgtata cgagtttctt ttgcggatcc gacagctcgc
     3541 tcagcctgcg ttcctcgttc aacttttcca cgaaggcgtc gggatcggga ataccctccc
     3601 aaacgcaacg tgcgcgcatg gccatcaggc gggtttcctt gtctccccaa acgagcgtct
     3661 ggctgatgag actctcggtt tgcggacagc gattgtgagc caggatcagc aatccgaggg
     3721 catattctct tgaattggga tagcgagcgg cctgggctcg caacttgccg agcaggtgga
     3781 tgatcttgtc cgtgggaacg gcaaccttgc cctgttccag tgcctcaaga acaggctcca
     3841 gcaaacggct cgaggggtca aggaacttgt catttgtcag cacgccggca gcccgcgtgg
     3901 cgtcgatctt catcaaacac tttggcactt cgtgcgagac ctcgtcgtcg gccgagagga
     3961 tcgcgacgag cggctcgaac actgccgccc cgaaggcctt cgagaggcgg ttctcggaaa
     4021 cggcctcggc gatgccccgg gttgcgtgga cctgcaattc atggtccttg ccggtcagga
     4081 cgttcttgac cggctcggca cattcgtccg tggccatacg gccgaggcag aatgccgcct
     4141 ttctacgaat cgcgcccttc gggctgttca agagggtctt caactcgcga gccgcaatct
     4201 cgggttcaaa cttgatgagg agttcgacga tttcgttgac ggggcattgc cagccgcggc
     4261 tttttcttcg gccttggtgg aaccgaggat ctttcagggc tttcagcaga tgcggaacac
     4321 agtccttgcc ttcggccagc aggttcctgt aggcgaccgt gcacgaatcc caatccagaa
     4381 ggtcgtcgac catgtcgtga atgcgggcgt cggtcattcg ttggacttcg ccgtttcggc
     4441 cgccgtccgc cgggtcctcg aagatcgtgg ccgggcgcgg aagcggtccc tcggcgcggg
     4501 cgccgctgac gagaacctct cccaagagta agagccagag aaggaataga tgcttcatgg
     4561 tcacctctcc tcgccagagt tctgccgctt atttgactct ggcgggtggg cgacggtcaa
     4621 ccggcggacc ggatcgccgc gcacaggtcg gccacttggc ggcaggcagc gacatcatgg
     4681 acccggacgc attcgacgcc ggccaggaca caggccgcca cggccgccag cgtgccgtac
     4741 aagcgtttga tcggttcggg aatacccagt acctctccta taaatcgttt gcgggacggg
     4801 cctataagta ccgggacgcc caaatcgcgg aactcgcccg cacggcgcag gatttcgagg
     4861 ttatgggtcg tggtctttcc aaagccgatg cccggatcga cgacgagtcg ctcaagcggg
     4921 attccggcgg tggttgcggc gacgatacgc tcggccagga actggcggat ctcggcaaca
     4981 acgtttcggt aggtcgggca ttgctgcatg gtggccggtg tgccttgcat gtgcatgagg
     5041 atcaccccga cgccggtgga ggcggccaag ggtgccaagg cctggtcgtc gcgcagcgcc
     5101 gtgatgtcgt tgatgatggt cgcaccggca tccaccgcag cccgggccac ttcggccagc
     5161 cgagtgtcga cggagatgga cgggccccgg gagccgaact gccgggccaa ttcagcgatc
     5221 accggccggg tccggcgaat ctgctcgtct gcgggcacag gttggcttcc ggggcgggtt
     5281 gactcgccgc caatatcgat cacgtcggcg ccttcttcgg ccattctgac gcccgcctcg
     5341 atggcctctg aagcgcgggc atacctgccg ccgtcgctga atgagtcggg tgtgacgttg
     5401 agcactccga acacgaaggg tcctgccccg cgaacgagcg gtctgccttt gatgtggagc
     5461 acctgctggg acatcatggg gcacattata gacggggaat cgctcctggg catacggatg
     5521 tcagcttcac aagagcaccc gatgaagccg ctcgtagagt gcgataaaat gccttgaata
     5581 aaggagttat agttgatcgc tcctattgtt ctggttggcg gtaaccctcg tgtgaatttg
     5641 caggcgtcaa aaaaccgaaa attttcctaa ccaaacgctt gcctaactta cgatgcgttg
     5701 tatactgtca tcgtcattgg cgaatcgagg tactttttca ggacgcacga atcatgaccc
     5761 agagcacaac gactgcaaca ggtggcaacc gggagcaggc cctggcccgg gccctgcaac
     5821 agatcgagaa gagcttcggc aagggcgcga tcatgaagct cgaccagcac gaaacgacgg
     5881 tccatgacgg tatcagcact ggcacgctgt cgctggatct ggcgctgggc ggatatggga
     5941 ttccgcgcgg tcgcgttgtg gagctattcg gaccggagtc gtgcggcaaa accactctgg
     6001 ctctgcacgt tgccgccagc gcgcagcggg ccgggggtgt ggcggcggtc atcgatgctg
     6061 agcatgccct ggatccgacg tgggccaagc gatgcggcgt gaagcttgag gagctgctgg
     6121 tcagccagcc ggatacggga gagcaggcca tggaaatcgt cgagatgctc gttcgctcga
     6181 acgcggtaga ctgtatcgtt gttgactccg tggccgcact ggtgcccaag gcggaattgg
     6241 agggtgaaat agggcagtcg catgtcggtt tgcaggcgcg gctgatgagc cagtcgctgc
     6301 gcaagctcac cgggatcatc tccaagagca agtgcgtggt gatcttcatc aaccagcttc
     6361 gcgagaagat cggggtcatg tttggcaacc ccgagacgac gccgggcgga cgggcactca
     6421 agttctattc gtcgatccgg ctggacatgc gcaagatcac gacgctgaag gagggcgaga
     6481 cggccatcgg caaccgggtg agggccaagg tcgtcaagaa caaggtcgcc gcgccgtttc
     6541 agtcagcgga gtttgacatc atgttcaatt cgggcatcag cagcgaaggc gacctgatcg
     6601 acctggccat cgctgaaggc ctgatcgaga aaaccgtcgc gtggatcagc tatggacagg
     6661 ttcgtttggg ccagggtcgc gaaaacgcga agcagtttat ccaggagaat ccggacctgg
     6721 ccaacgagat ccgcgagaag atacttgcgc ggcggcggcc ggccgcgact cccgcctccg
     6781 gtgacggcaa ggttccttct gaaacctcga aggcaacggc tgtcaccaag gaggatcccg
     6841 cggcgaagga caggggcaag gctaagccaa agtttccgcc gcccaaaggc aagcgttgaa
     6901 tgatggtgac gtgacgcgac ggcagggatt cggtgggcga tcgcgtaggg cacgggcggg
     6961 tgcggctctg gcaccggccc cgtgcgtcct tgatgggaac gggacgggct tgtttcccga
     7021 ggattcatca tccccaaggg cagatgagta cctggaagga aaccgggacg caagcctgtc
     7081 ccgttccttt tattctgtca atcggtaccg cgggtcgcgg tactgtgggc ggctccttca
     7141 caggctcgag cgctcgccac ggcgaagccg gtaagcgggg ggccccggac cgactcccac
     7201 aagcgggaca agggcaaggc aggagcgcga agcggcatcg ctacgggggg actggtcctt
     7261 tcctgtttcg ccaaggctgc acagggcggg tgcggtcgcg gttttgaaag agccccctgg
     7321 aggaaccatg gaccagccgc accgaattgg ccgcggaccc ggttcccgct cgacactgct
     7381 gcctgctggt tatcgcaacc aacatgtgac aggtattgac ctttcgagaa caagatgctg
     7441 gcggtagaat gcgaacccgt cgggcggcgg gcgtcaggtc gcccaagttc aaggggagca
     7501 acattcagcg attggagaac gcgccatgtc atctatgttt ggactggttg tttctgcgcg
     7561 ccatgtcttg gtatcagcag tgacccatgt gctgcttgcc ggcctgcttt tgtccgcaac
     7621 accccgggct gaggcacagg atgcggccgc ccgtttgaca caaacatcag caatctcgaa
     7681 gctgcctgag attcagcggt tgggcgatga caccacggcc tatgtgtttc gaatgggctg
     7741 ggaggaactg aaaccccaac tggccggcac ccaggccctg gaggccatcg aggacccgca
     7801 gatacaagcc ttcttcaagg cattcaagac tgccggcgag cggtcgcatc cggcgtctcc
     7861 tttctttcag tttctgctgt cggcgttgca cgaccgactc gtgatggtca cgcggccgat
     7921 accggggggc gagacccagt cgagtgaaga agaggaggag caaggaaaaa cggccaaacc
     7981 cggaacccag aacttcgcga tcatcactcc cggcccgcag ggcaaggcgg cgttcgggga
     8041 actctatggc aaagcaatcg gggcgctcaa ggaggcggcc gaggcggaat tggaaaagga
     8101 gaagatcgga tccctgacct tcgacgtgtt ggagaagacc ggcacgggcc tgcacaccgc
     8161 ctggaacaac gaacaatgct actgggccgt gggggcgggc gcggcccgat gggcggcgga
     8221 atcgtccgct ccggcacgct cgctcgatcg ttccgagctg tttcagacat cgatcggacc
     8281 gctcttcaag gggcaacaac ggcaaccgat cgcgatgtac tactatgacc tacggccttg
     8341 gtggcgcaag gtcgacggca tggatcatca ggggatgtgg aagcaggtgt cgtggcacag
     8401 cctggacgcc gtggccggtg caaccttcat tgagaaggat cactatcgca accggcatta
     8461 ctggcggatc ggcccggagc gatacgggct ctttaaggac agccgcacgg cccgcatcaa
     8521 cgaggcctgg ctcaaacgtg taccggcgga ctccagtggg tttaccaccg gtgtctacga
     8581 cgcctgcagc ctcatggcgt tccttcccac gatcttcatg cacctgtttg gtgctgatga
     8641 gaagagcatc atgcaggcgc cgatgttcga gatgccgttt cagcccgtgc tcaaggaact
     8701 cgggccgcgg tacctgatct accgcatgcc ggggcgctac ggcagctttc cgatagccga
     8761 cgtgttcccg ctgagcaatg cggttgtcat ttcggaggtt cgcgatgcag cggcattcaa
     8821 ggaagccttg cagaccttcg cgattgggcc ggtgcaacct cacacctcga ctgttctcgg
     8881 ccgaacagtc atcagcatca acgtgatgta tttcacgatt tacttcgcgg tgttcgacaa
     8941 cgaggtgctg atcacgatga acccgcaact gctcaaggac gcgatcgagg ctgccgaacg
     9001 gccggggcca tcggtgattg agaccgccgc atacaaggaa gcagtgcggg gtgtctcgcc
     9061 cgacgcgtgt ttcctgctgt acatgccgcc aggcgggttt tcgcggggca tctacgacca
     9121 ttacattccg atgcttcaac agacgattgc tttggccggg gcgtttcgcg gattcgattc
     9181 caagcagaag cccgcttcca cgctcgatcc gatcgtgttc cccagggggt ctgatatcgc
     9241 ccgccatgcc aggcaggcga cgatactgag cgccgtcgat gatggcgaag gggtcttgtt
     9301 cgacggtact gctcccattc tgaccactcc ctacttgggc ctacgtgcat gcgttgaaca
     9361 ggttgcttcc gatcgagggc gagtatccac ctttgatgct ggcggttgtg ccgttcattg
     9421 gcgtacctga ttgactggcg gcctgatccc ctgcgacacc gctggaaaac gcaggaccgg
     9481 cctcgtattg tacgaatgcg gatgttgcct ggtgggtttg gccacgggtg aggctcggcc
     9541 gccctggagg acgggcaaca gcgttctgag cggtcatgcg gattgtgttg atccacagcg
     9601 gcgggttgga ttcgacggtg ttgctgtaca agctgcgcgc cgaaggtcat gaagtgcgct
     9661 gcctgggagt cgactacggg cagcgacacc ggcgggaact cgacgcgggg caggccatct
     9721 gtcgcgatgt gggcgtcgaa taccgcgttg ccgacctgag gggtttgcgg tcgctgctgg
     9781 ccggaagtgc cctgactgac tcgatcccgg tccccagcgg ccattacacc gacgagacga
     9841 tgaaggccac catcgtgccg aaccgcaaca tgatcatgct ttcagtggcg atcgggtggg
     9901 cggtgagcct gaggtacgac gccgttgctt acgcggccca cgcgggcgac cacaccatct
     9961 accctgattg ccggccggtt tttgtggagg cggttcgcag cgttgctcgg ttgtgcgact
    10021 ggcggccgat cgagttgctg gcgccgttcg taggcaagag caaagccgat attgtccgta
    10081 ttggggcgga gctcggcgtg cccttcgagc ggacatggtc gtgctacagg ggcggagagc
    10141 ttcactgtgg cacctgcggg acatgcgtgg aacggcggga agcgttccag aaagccggta
    10201 ttgcagatcg gacggtgtat cgcggacaat aggttagccg ggtttgggcg accggcagga
    10261 gcacgaggat ggacggactg caccatctgg ggaaaccgtc gaaccagccg acggagaagg
    10321 tggacctgat cgaatggtcc ggccagccga tgaccgtgcg cctggagtgc tcggagttca
    10381 cgtcgctgtg cccggtgacc ggtcagccgg acttcgggca aatcatcatc gagtatgtac
    10441 ccgggcgatt tctggtcgag accaagtcgc tcaagctata cctgtggaag tatcgcagcg
    10501 agccggggtt caacgagagc ctggttgaca ggatcgcctc cgacctcttc gagcagatcc
    10561 ggccgttgtg gctgcgggtg agggggcaat tcaacccccg cggcggcatc cgcgtcaccg
    10621 ccaccgccga gcgaggagac aaccggcacc gaccgtcatg accacgatcg ttcgcaccat
    10681 tcaattcagc gccggtcacc gcctgcggca acatgagggc aagtgcgccc atgttcacgg
    10741 gcacaactac acggtcgagt tccacgccac tgccgagcag ctcgacagcc tcggccgggt
    10801 gatggacttc agcgtactaa gagatcgact cggcggctgg atcgaccaac attgggacca
    10861 cgggttcatc tgtagcaaag acgaccggga ggtgcttgat gcgatggctc tggtctcggg
    10921 gcagaagctc tttgtgatgg aggtcaaccc gacggcggag aacctggccc ggtacttgct
    10981 ggacgtcgtc gggccggagc aactcgcggg cacgggtgtt cggttggtgc gggtcgttct
    11041 tgccgagacc gaaaactgc
//
LOCUS       DAOTPW010000188        11050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5568_length_11050_cov_5.511960, whole genome shotgun sequence.
ACCESSION   DAOTPW010000188 DAOTPW010000000
VERSION     DAOTPW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5568_length_11050_cov_5.511960"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="P5316_15735"
     CDS             complement(<1..2040)
                     /locus_tag="P5316_15735"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRR86460.1"
                     /translation="MSDITGARSFSQRANTAECRTSRRRRVLIIAGLCSAIGAGAHAA
                     DPVWITATGPSCPLMRKEFKLGTAPSKAVVRIVGLGHFELRVNGKRAGDSLINQPWSQ
                     YDKTIYFQEFDIAPLLVRGDNVLGVMLGNSFWYNPPSPAGRYHKGGAECTFGTQYLMW
                     LDADLETADGGRQRIVSDGTWKTSPGPVTFSHVYGGEDYDARLDQPGWDSPGFDDKAW
                     TPAATADPPAGKLARQFWPPIREKEVFPAKDFVPAGEGVFHVFFGQNASGIVRFTVEG
                     KAGQSFTLQPSEYRGDKGEFMKPRWGAPVLFRYTLKGGGPETHQWLFHYNGFQAVEMV
                     GAVPADRPNPKGLPVVQRIELLHVRSDLPQVGAFETSSKLYNDTHRLVDWAMRSNMTY
                     VMTDCPHREKLGWLECAHLCAPMFSYRYDCRDWLAKIARDIRDAQEPSGRILTVAPSY
                     PGTRFPDAFHWTVEWGAAGALLPWHHYVWYGDPQILRDNYDCMCRFVDFVASESKDGI
                     APAGLGDWYDYGHGRPPGPSRFTPQDLTATAVQVMCIDAVIHTAEVLQRADDVRRYRG
                     LRDKTAEAFLRKFYDPQKRMFANHGSCQCAHTIALTAGLVPEADRQAVLSAVIEDLAK
                     RDWQQTPGDVGHVFFIRALAEAGRSDVLHRVYSRDGLGSYGGILKKGLTAMPETWD"
     gene            2223..4292
                     /locus_tag="P5316_15740"
     CDS             2223..4292
                     /locus_tag="P5316_15740"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRR86461.1"
                     /translation="MTTLRIALVLIVGLASQAASQAVTARPDEMAAARQWVAVRLRGE
                     EAERPATAEPPYSFIYGGRSSSELLPTWKPKRTSPRVDQNRVGLSLTYTDPQTGLVVR
                     CEAVEYADFPTVEWTLYFKNTGTSDTPILEQIHAMDIRLERPKECEFVLHHNRGDSCA
                     ANSYEPLRTPLGPGTELRFAPDGGRPTNGQWPYYNIQWGDQGLIVAIGWPGQWASQFT
                     RDKETGLRVRAGQELTRLKLHPGEEIRTPLVVMQFWSGDKIRAQNVWRRWMRAHGMPR
                     PGGQPVRAQTAACSSHQFGEMINANEENQKFFVDRYVEERLPLDYWWMDAGWYPNDPR
                     YHAAGWPNTGTWEVDHKRFPNGLRAISDHARSKGIKTIVWFEPERVTPGTWLWRNHQD
                     WLLGPPADPNQDVADRSKPADKLLNLGNDTAREWLTNHVDKLLTDQGIDLYRQDFNTD
                     PLGRWRANDAPDRQGITEIRYVTGYLAYWDELRRRHPDMLIDSCASGGRRNDLETMRR
                     AVPLLRSDYILEPVGQQNHTYGLSFWLPFYGTGMSREGMYDSRSCFCPHITGCYDMRD
                     KKDDYERARRVMSDWKQVAPCMLEGDYYPLTPYNVANDVWMAWQFDLPEKGEGVVQAF
                     RRGESICESARFPLCSLQADATYIVTDLDTDKPVSISSQALMKEGLPVEIRGRPGSRI
                     VRYERHR"
     gene            4666..7845
                     /locus_tag="P5316_15745"
     CDS             4666..7845
                     /locus_tag="P5316_15745"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HRR86462.1"
                     /translation="MSRRAFLASTGLAIAGTGAIRAIAGPFNPADVTDFPVPADKKLD
                     PLWVRSLFERGAPTIYRSAAGELEHIGMPIGGICTGQLYLGGDGRLWHWDIFNQPAPQ
                     EWRNGSGPHYAKPAEVKSPIEQGFAIRVVTTDRAQIRPLDQRGFRDVRFRGEYPIGLV
                     EYRDVDVPVVVNLEAFSPFVPLNEDDSGLPATVLRYTVRNIGDSPVEVELGGWLQNGV
                     CLASGQPGRGQRENRILRQPAMTVLECRARANPAASKPVAEARPDIVFDDFESETYDK
                     WSVTGTAFGDGPIATSQIASYQGDVNAHGQRVVNSHSSRRGGGISATDSHIGSMTSKE
                     FTVERHYIRFRIGGGAHPGQTCVNLLVDGAVVRSAAGHNNNRMRIEHFDVREFAGKQA
                     RLQVMDGVKDAWGNIGVDEIVFTDGQKDELQMPEQQPDDGTMALALLGNSEGVFADAD
                     LPAGPFPIPLFKAQDGRAEASRDFGQRLIGAIGGKRRLAAGEEVEFTFVLAWHFDGLW
                     WDSLPFLENVRNLRRFYGTRFRDAADVVRYVAREFDRLLSQTRLWRDTWYDSTLPYWL
                     LDRTLANISTLATATCYRFDNGRFYGWEGNYCCGGTCTHVWQYAQAVGRLFPALERST
                     REMIDFGLAFDDATGIIHYRAEAHRELAVDGQAGTILRVYREHQMSKDDGFLRRIWPR
                     VRKSVELLMSRDTDHDGLLDGAQYNTLDAAWYGQIAWISSHYLAAVRAGEKMAVEMGD
                     AGFAERCRKIVDRGGKRLVERLYNGEYFIHIPDPDKPQTVRTGDGCHIDQLFGQSWAH
                     QVGLGRIVPLEPTLSALRSLWRYNFAPDVGPYRLMAADKIPGGRWYAMPGEGGLLMCT
                     WPKGGMETASGGGKDAGFAGYFNECMTGFEYQVAAHMIWEGMVQEGLAITRMIHDRYH
                     ARKRNPWNEIECGDHYARAMASYGVFLAVCGFEYHGPQGYLAFAPRMSPDHFRSAFTS
                     AEGWGTFEQNRQNDSQTETIVVRHGRLTLRKLAFDLAAGFRPGSVAVSAAGQSLPASM
                     SQDGSRVTITLDAVHVLKTEERLDIIFRA"
     gene            8248..>11050
                     /locus_tag="P5316_15750"
     CDS             8248..>11050
                     /locus_tag="P5316_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR86463.1"
                     /translation="MTSFPVVNSSTVVADDATPYTVSTTFSDPDGYDSIRCIRVLFNY
                     TEAGGDQSQGRGYMNWGKTDADVTQWGGTWVIADATGGGRWAYRTDAWGGITYITPLS
                     CETTVTGKATGGSGARTVTWTFTVKPAWAFNPVMNDADAWAADGVIGGTSYIVGWIDG
                     QMSFDVVSEPCSATCTTPRAPVVSNPRNNAVDVSIDPGDSPDDVYAIMISPGLGGKTY
                     VQSDGTLGPRPQWSTKSTWGNKTVTGLLPRRTYTFSVRASRSLAGYCPSAWGPGSQVT
                     TTGDIPIIDYYQGKAFSSWVRGQCPYRSVSTSAWSPLWDLAAGSMGRGLAGGLDADTY
                     DWRNVDSGSGWGTPTWSGAFTTLEFLQAARDRNAAPVLTANLFGGGYRDWSNPNYPGV
                     FVCQTANPEGLAADWVRYTNFIVQSYRQGDEASLAGEDLRVYNSIVNWGTKPKLLTPA
                     EGSVPPVKYWEIGNEPELGGYGDFLTNHYLGPADYRDRYKTISAAMLAVDPSLKFGPC
                     LINPSDPNGSGQWLSALAADPGVRLDFVAYHPYYGAIKSNWGWFEGLATALRDYKKYL
                     NDHTAGMRSILAQNGRTSCDFIASEWNPVNWDAPGTLQASVANAIGVVETCFTFAEDG
                     VLAGTFWEQPQSKLGPKLAFQGLVSHMGNVLITTGAQMGFDPANSNFRFYVTKNEGDD
                     STIMIWGLNFDDTQPITVNMGLIPCQVTSAKLRHLGKPGDDDAGGDTSLTDYSGLGWE
                     EEDVTSGFTPQDFAFTMEDAEITLLVLEVAPVDDDLDGVFDHLDNCMGVYNPGQEDQD
                     GDGLGDACDNCPAVANPGQEDLDGDLVGDVCDDDVDGDGILNPQDNCPLIVNPEQENS
                     DGDALGDACDACPGTATGAPVDAHGCPTPVTGDFDRDGDVDLEDFGHFQACLSGLSVP
                     QENPDCQNANLDGDSDVDKA"
BASE COUNT         2153 a         3329 c         3559 g         2009 t
ORIGIN      
        1 gtcccaggtt tcgggcatgg cggtcaggcc ttttttgagg atgccaccgt agctgcccag
       61 gccgtcgcgc gagtagacgc gatgcagcac gtcggaacgg ccggcctcgg ccaaggccct
      121 gatgaagaac acatggccga cgtcgccagg agtctgctgc cagtcgcgtt tggcaagatc
      181 ttcgatgacg gcgctgagga ccgcctgcct gtcggcctcc gggacgagtc cggccgtcag
      241 ggcaatggtg tgggcgcact ggcacgagcc gtgattagcg aacatacgct tttgtggatc
      301 atagaacttt ctgaggaaag cctctgcagt cttgtctcgg aggccgcgat aacgccggac
      361 atcgtcggct cgctgcaaga cctcggccgt atgaatgact gcgtcgatgc acatgacctg
      421 cacggcggtg gcggtcaggt cctgcggggt gaagcggctg ggtccgggcg gccggccatg
      481 gccgtagtcg taccagtcgc cgaggcccgc gggggcgatg ccgtctttcg attcggaggc
      541 cacgaaatcg acgaaccggc acatgcagtc gtagttgtcg cggagaatct gcggatcgcc
      601 gtaccagacg tagtgatgcc aaggcaggag agccccggcg gcgccccatt caacggtcca
      661 gtggaacgcg tcggggaaac gcgtgccggg gtacgacggt gcgaccgtga ggatacgtcc
      721 ggagggttcc tgagcgtcgc ggatgtctcg ggcgatcttg gcaagccagt ctcggcagtc
      781 gtagcgatag ctgaacatcg gcgcgcacag gtgggcgcac tccaaccagc cgagtttttc
      841 gcgatgcggg cagtcggtca ttacgtaggt catgttggag cgcatggccc aatcgacgag
      901 gcggtgcgtg tcgttgtaaa gcttgctgga cgtctcgaac gcgccaacct ggggcagatc
      961 gctgcggacg tgaagcagtt cgatgcgttg aacgacgggg agtcctttcg gattgggccg
     1021 gtcggccggg accgccccca ccatctcgac cgcctggaag ccgttgtagt ggaaaagcca
     1081 ctgatgcgtt tcgggcccgc cgcctttgag cgtgtagcgg aacaggactg gagcccccca
     1141 ccgcggtttc atgaactcgc ctttgtcgcc gcgatactcg gagggctgga gggtgaagct
     1201 ctggcctgcc ttgccttcga cggtgaagcg gacaatgccg gaggcgttct ggccgaagaa
     1261 gacgtggaaa acgccctcgc cggcggggac gaaatccttt gcagggaaga cttccttctc
     1321 acggatgggc ggccagaact gtctcgccag tttgccggcg ggcgggtcag cggtcgcggc
     1381 cggggtccag gctttgtcat cgaagcccgg cgagtcccaa ccgggttggt caaggcgggc
     1441 gtcgtaatcc tcgccgccgt agacgtggct gaaggtcacc gggccgggtg aggtcttcca
     1501 ggtgccgtcg gacacgatcc gttggcggcc gccgtcagcc gtttcgagat ccgcatcaag
     1561 ccacatcagg tattgggtcc cgaaggtgca ttcggctccg cccttgtgat accgcccggc
     1621 cggtgacggc gggttgtacc agaaggagtt gccgagcatc acccccagaa cgttgtcacc
     1681 tctgaccaga agcggcgcga tatcgaactc ctggaagtag atcgtcttgt catactgcga
     1741 ccagggttgg ttgatcagcg aatcgccggc tcgcttgccg ttgacgcgca actcgaaatg
     1801 gccgagaccc acgatccgca cgacggcctt tgacggtgca gtgccgagct tgaactcctt
     1861 gcgcatcaaa ggacaactcg gtcctgtggc cgtgatccag actgggtcgg cggcatgagc
     1921 gccggcgccg attgcagagc agagaccggc gatgatcaga acccgccgtc gccggctggt
     1981 ccggcactcg gcggtatttg cacgttgact gaacgacctg gcgcctgtta tgtcagacat
     2041 gatcgccctc atcaatccga gactctcaca tcagcatacg acggcgcgga tcatacacgc
     2101 acggcggcga aagcgacaac atctccaaga accgaggagc ggggtttgtg tcaaccgtcg
     2161 gcgcagatag aatatcgctt cgcagctcat tggacgaagg aacatcgatc gggagccgca
     2221 tcgtgaccac gttgagaatc gcattggtgt tgattgtcgg tcttgcttct caggcggcga
     2281 gtcaagcagt gacggcacga cctgacgaga tggccgcggc cagacaatgg gtggcagtga
     2341 gactccgcgg cgaggaggca gaaaggcccg ctaccgccga accgccctac tcgttcatct
     2401 atggaggacg atcgtcttcg gagctactgc cgacgtggaa acccaagcga acaagccccc
     2461 gcgtcgatca gaaccgggtc ggactgtcgt tgacctacac cgatccgcaa accggtctgg
     2521 tggtgcgctg tgaagcagtc gagtatgcgg acttccctac ggtggaatgg acgctgtact
     2581 tcaagaacac cggcacgtcg gacacgccga tactcgagca gatccatgcg atggatatcc
     2641 ggttggagcg gccgaaggaa tgcgagttcg ttcttcacca caaccgcggc gattcctgcg
     2701 cggccaacag ctacgagccg ctgcgaacgc cgctgggccc cggcacagag cttcgctttg
     2761 cccctgacgg cggacggccg accaacggcc agtggccgta ctacaacatc cagtggggtg
     2821 accagggtct catcgtcgcc atcggatggc ccggccaatg ggcgtcgcag tttactcgcg
     2881 ataaggaaac ggggttgcgg gtgcgcgccg ggcaggagct gactcgcctc aagctgcacc
     2941 ccggcgagga gatccgcacg ccgctggtcg tcatgcagtt ctggagcggc gacaagattc
     3001 gcgcccagaa cgtctggcgg cgctggatgc gagcacacgg catgccgcgt ccgggtggac
     3061 aaccggtgcg agcccaaacg gcggcgtgca gctcacacca gttcggcgag atgatcaacg
     3121 caaacgagga gaatcagaag ttcttcgtcg accgctatgt cgaagagcgt ctgccgctgg
     3181 actactggtg gatggacgca ggatggtatc ccaacgaccc tcgctaccat gcagccggtt
     3241 ggcccaacac aggcacctgg gaggtggacc acaagcgctt ccccaacggg ctgcgggcaa
     3301 taagcgatca cgcgcgaagc aagggcatca agacgatcgt ctggtttgag cccgagcggg
     3361 taacgccggg cacgtggctg tggaggaatc atcaggattg gctgctcggt ccaccggccg
     3421 acccgaacca ggacgtggcc gatcggtcga aaccggcaga caaactgctg aatctcggca
     3481 acgacaccgc acgcgagtgg ctgaccaacc atgtggacaa gctcttgacc gaccagggca
     3541 tcgacctgta ccggcaggat ttcaataccg acccgctcgg gcgatggcga gccaacgatg
     3601 cgcccgatcg ccaaggcatc accgagatcc gctatgtcac gggctacctg gcgtactggg
     3661 acgagttgcg gcgccgacac cccgatatgc tgatcgactc atgcgcctcg ggcggccgtc
     3721 ggaacgacct ggagacgatg cggcgagccg ttcccctgct acgcagcgac tatatcctcg
     3781 agccggtggg tcaacagaat cacacgtacg ggctttcgtt ctggctgccg ttttatggga
     3841 cggggatgag ccgcgaggga atgtatgatt ctcgcagttg cttctgcccg cacatcaccg
     3901 gctgttacga catgcgcgac aagaaagatg actatgagcg ggctcgtcgg gtcatgagtg
     3961 attggaaaca ggtggcgccg tgcatgctcg aaggagacta ctacccgctc acgccataca
     4021 acgttgccaa cgacgtctgg atggcatggc agtttgacct tccggagaag ggcgagggcg
     4081 tggtgcaggc gtttcgccgc ggagagagta tctgtgagtc ggcacggttc ccgctctgca
     4141 gcctgcaggc agatgcgacg tacatcgtca ccgacctgga caccgacaaa ccggtgagca
     4201 tcagtagcca agcgctcatg aaagaaggcc tgccggtgga gattcgcggc cggccggggt
     4261 cgaggattgt ccgatacgag cggcacaggt aaggcaggat ccggcaacgc cgacagtcgg
     4321 aaaatggaga ttactcgaga gatgagcgat aacaacggcg ccaacgacaa cgataatgca
     4381 aagcagatgg atgtaactcg gcacccctta tccaactctc ccgcgattcc gacacacccg
     4441 cagccatctt gccccgaagg ggcgacagaa ggtccgatcc accaacgtga acctgtcgcc
     4501 ccgctggggc tgactgaccc atgcggcact ttttccaggg gctcacgccc ctggctacac
     4561 accggcgccc ctgccggggc gggttctact ggtactttcc aggagccata ccccaatcct
     4621 attcctcagg gttgttgcga gagtcgatgc tccagcagcc atgacatgag ccgcagggcc
     4681 ttccttgcct caacaggcct ggccattgcg ggcacaggcg caatcagggc catcgccggg
     4741 ccgtttaatc ccgccgatgt gaccgacttt cctgttccgg cggacaagaa gctcgatccg
     4801 ttgtgggttc gttcgctgtt tgagcgcggc gcccccacca tataccgatc tgccgccggc
     4861 gaactggaac acatcggtat gcccatcggc ggcatctgca ccgggcagct ttacctggga
     4921 ggcgacgggc ggctgtggca ctgggacatc ttcaaccagc ccgcgcccca ggaatggcga
     4981 aacggcagcg gtccgcacta tgccaagccg gcggaggtga aatcgccgat cgaacagggt
     5041 ttcgcgattc gagtcgtcac gacagatcgc gcccagatcc ggccattgga tcaacgcggc
     5101 ttccgggacg ttcgctttcg aggggaatat cccatcggtc tcgtcgaata tcgagacgtc
     5161 gacgtgccgg tggtggtcaa cctcgaagca ttctcgccct tcgtcccact gaatgaagac
     5221 gactccggtt taccggccac ggtgttgcga tacacggttc gcaatatcgg tgactcgccg
     5281 gtcgaggttg agctcggtgg ctggctccag aacggcgtat gcctggcgtc gggacagcct
     5341 gggcgggggc agcgtgagaa ccggattctg cggcagccag ctatgaccgt gctcgaatgt
     5401 cgggctcggg ccaatccggc ggcatcaaag ccggtcgcgg aggccaggcc ggacatcgtg
     5461 ttcgacgatt tcgagtccga gacatacgac aaatggagcg tgaccggcac ggcgttcggt
     5521 gacgggccga tcgcgacttc gcagatcgcc tcctatcaag gcgacgtcaa cgcccacggc
     5581 cagcgggtgg taaactccca cagttcccgc cgcgggggcg gcatctcggc cacggattcc
     5641 cacatcggca gcatgaccag caaggaattc actgtcgagc gccactacat ccgctttcgc
     5701 atcggcgggg gcgctcatcc aggtcagaca tgcgtgaacc tgctggttga cggagcggtc
     5761 gtccgcagcg cggccggcca caacaataac cgcatgcgga tcgagcattt tgacgtccgt
     5821 gagttcgccg gtaagcaggc ccgcctgcag gtcatggacg gcgtcaagga tgcctggggc
     5881 aacatcggcg ttgacgagat tgtgtttact gacgggcaga aggacgaact ccagatgccg
     5941 gagcagcaac ctgacgacgg cacgatggcc ctcgctctcc tgggtaactc ggagggcgtc
     6001 ttcgccgacg ccgatttgcc tgccggcccg tttccgattc ctctgttcaa ggcgcaggat
     6061 ggccgggccg aagcgagccg cgacttcggc cagaggctga tcggcgcgat cggcggcaag
     6121 aggcgtctgg cggcaggcga ggaggtggaa ttcacttttg ttctcgcctg gcacttcgac
     6181 ggcctgtggt gggactcact gccgttcctg gagaacgtgc gaaacctgag gagattctat
     6241 ggcacgcgtt tccgcgacgc ggcggacgtc gtgcgttacg tggcccgcga gttcgatcgg
     6301 ctcttgtcgc agacgcgcct ctggcgcgac acctggtacg attcgacgct cccctactgg
     6361 ctgctggatc gaacgttggc gaacatctcc acgctggcca cggcgacctg ctatcgcttc
     6421 gacaatggcc gtttctacgg ctgggaaggc aactactgct gcggcggcac ctgcacgcac
     6481 gtttggcagt atgcccaggc agtgggccgg ctcttccctg ccctcgaacg cagcacgcgg
     6541 gagatgatcg acttcggcct ggcgttcgac gatgcgacgg ggatcattca ctaccgggcg
     6601 gaggctcatc gcgaactggc ggtcgacggg caggcaggca cgatcctgcg agtttatcgc
     6661 gaacaccaga tgtccaaaga cgacggtttc ctgcgccgca tctggccgcg agtacgcaag
     6721 tcggttgagc tgctcatgag ccgcgacacc gaccacgacg gactgttgga cggcgcccaa
     6781 tacaacaccc tcgacgccgc ttggtacggg cagatcgcgt ggatctcctc gcattacctc
     6841 gcggccgtgc gcgccggcga gaagatggcc gtcgagatgg gcgatgccgg gttcgccgag
     6901 cgatgcagga agattgtcga tcgcggcgga aagcgactgg tcgaacgcct ctacaacggc
     6961 gagtacttca ttcacattcc tgatcccgac aagccacaga cggttcgcac gggcgatggg
     7021 tgccatattg accagttgtt cgggcaatca tgggctcacc aagtcggctt gggacgaata
     7081 gtgccccttg agccgaccct ttctgctttg cgatcgttgt ggcgatacaa cttcgcaccc
     7141 gacgtcggcc cttatcgcct gatggctgcg gacaagattc ccggcgggcg atggtatgcc
     7201 atgcccggcg agggcggctt gctcatgtgc acgtggccca agggcggcat ggaaaccgcc
     7261 agcggcggcg gcaaggatgc gggcttcgcc ggctatttca acgagtgcat gactgggttc
     7321 gagtaccagg tcgcggctca tatgatctgg gaagggatgg tgcaggaagg cctggccatc
     7381 acccggatga tccacgaccg ctatcatgcc cgcaagcgca acccctggaa cgagattgag
     7441 tgcggtgatc actatgcccg agctatggcc agctacgggg ttttccttgc cgtctgcggt
     7501 ttcgagtatc acgggccgca gggctacctg gcgtttgccc ctcgcatgtc gcccgatcac
     7561 ttccggtccg ccttcacatc ggctgagggt tgggggacgt tcgagcagaa caggcagaac
     7621 gactcccaaa ccgaaactat cgttgttcgg cacggccgtc tgactcttcg caaactggcg
     7681 ttcgacctgg ccgccggctt tcgcccgggg tccgtcgcag tctcggcagc gggtcaatct
     7741 ctgccggcca gcatgagcca ggatggttct cgggttacca tcactctcga cgcagtacac
     7801 gtcctgaaaa ccgaggagag gctcgatatc attttcaggg cctaggaaac aagctgagtc
     7861 cggcattcgc ctgcaacgca gagcactttg cggcaatcaa cctaaatcct ttggcgggag
     7921 tatgttaaga tcgtctcctg gtgtccgcat ggcaaaggtc cgcaaaggac gatggcgcag
     7981 gcgtagcagg agccgacagt tctgccctct cgccagccga atgacggacg gctgataacc
     8041 tggcttcggt caggcaaaga atccggccgc agatccttct cacccacaag acgtttttgc
     8101 tccatgtgtt acaatactca tggtagtcgc gtcgcggatg tcggagagac tgagccggtg
     8161 aacaaaggtt tttgggcttg gctgacgcag gcatgcgtcg tgcttacggt gttcgctgct
     8221 tcaagctggg gggctggcgc gcccatcatg accagtttcc cggtcgtgaa ctcctcaacg
     8281 gtggttgccg acgacgcgac tccttacacg gtctcaacga ccttctccga ccctgacgga
     8341 tatgacagca ttcggtgtat tcgcgtgctc ttcaattaca ccgaggccgg cggcgaccag
     8401 agccaaggcc gcggctacat gaattggggc aagacggatg ccgacgtcac ccaatggggc
     8461 ggaacgtggg tcattgcgga tgccacgggc ggcgggcgtt gggcttaccg aacggatgct
     8521 tggggtggca tcacgtacat cacgcccctc tcatgcgaaa cgaccgtcac cggcaaagcc
     8581 acgggcggaa gcggcgcacg gacggtgacc tggaccttca ccgtcaaacc ggcctgggcg
     8641 ttcaacccgg tgatgaacga cgctgacgca tgggctgccg acggtgtcat cgggggaaca
     8701 tcgtacattg tcggctggat cgacgggcag atgtcctttg acgttgtttc cgagccttgc
     8761 tccgccactt gcacgacgcc gcgagcaccc gtggtgtcga atccaagaaa caacgccgtt
     8821 gacgtgtcga ttgatcccgg tgacagccct gacgacgttt atgcgatcat gatttcaccc
     8881 ggtctgggcg gaaagacgta cgttcagtcc gacgggacac tgggtccccg gccgcagtgg
     8941 agcacgaaaa gcacctgggg caacaaaacc gttaccggcc tgctgccacg gaggacgtac
     9001 acattcagcg tgcgggcctc aaggagtctg gccggctatt gtccctcggc gtgggggccc
     9061 ggcagccaag tcaccacgac gggcgatatt ccaattatcg actactacca gggcaaggcg
     9121 ttcagctcat gggttcgcgg acagtgcccc taccggtcag tcagcacctc ggcgtggagt
     9181 ccgctgtggg acctggctgc cggttcgatg ggacgcggcc tggccggagg tctggatgcg
     9241 gacacctacg actggcgaaa cgtcgatagc ggctccggct ggggcacgcc cacttggagc
     9301 ggtgcgttca ccaccctgga gttcctgcag gccgcacgcg accgcaatgc cgcgccggtg
     9361 ctgacagcca accttttcgg cggtgggtac agagactggt cgaacccgaa ctatcctgga
     9421 gtgttcgtct gccagaccgc caatccggag gggctggccg ccgactgggt tcggtacacg
     9481 aacttcatcg ttcaatcgta ccggcagggt gacgaggcga gtctcgctgg agaggatctg
     9541 cgggtctaca actcgatcgt gaattggggc accaagccca aattgctgac gccggctgaa
     9601 ggcagcgttc ctccggtcaa atactgggaa atcggcaacg agcccgagct cgggggctac
     9661 ggtgatttcc tcaccaacca ctatctgggc ccggccgact atcgcgaccg gtacaagacg
     9721 atcagtgcgg cgatgttggc ggtggatcct tcactgaagt tcgggccctg cctgatcaat
     9781 ccgtccgatc cgaacggcag cggacagtgg ctgtcggcgc tcgcggcgga cccgggcgtt
     9841 cgactggatt ttgtggccta ccatccctac tacggggcca tcaagagcaa ctggggctgg
     9901 ttcgaaggcc tggcaacggc cttgcgtgac tacaagaagt atctgaatga ccacacggcc
     9961 ggtatgcgat cgatcctggc ccagaacggc cgtacaagct gtgatttcat cgcctccgaa
    10021 tggaaccccg tcaactggga cgcccctggc acgctgcagg cctccgtggc gaatgccatc
    10081 ggcgttgttg agacgtgttt caccttcgcc gaggacggcg tgctggcagg caccttctgg
    10141 gagcaaccgc aaagcaaact tggcccaaag ctggcgtttc aagggctggt cagccatatg
    10201 ggcaacgttc tgatcaccac cggcgcgcag atggggtttg accccgcgaa cagcaacttc
    10261 cggttttacg tgaccaagaa cgaaggcgac gattcgacga tcatgatctg ggggctcaac
    10321 tttgacgaca ctcaacccat cactgtgaac atgggcctga tcccctgcca ggtcacgtca
    10381 gccaagctga ggcatcttgg caagcccggc gacgacgacg ccggtggcga caccagcctg
    10441 acggattaca gcggcctggg gtgggaggag gaggatgtga cctccggttt tacaccgcag
    10501 gatttcgcct tcacgatgga ggatgccgag atcactctgc tggtgctgga ggttgcgccg
    10561 gtggacgacg atctggacgg cgtattcgac cacctggaca actgcatggg cgtctacaac
    10621 cccgggcagg aagaccaaga cggagatggt ctgggcgatg cgtgcgacaa ttgtccggcc
    10681 gtcgcgaacc ccggccagga agacctggac ggggacttgg tcggggacgt ttgtgacgac
    10741 gacgtagacg gcgacgggat cctcaacccg caggacaact gcccgctgat cgtgaacccg
    10801 gaacaggaga attccgacgg cgacgcactg ggcgacgcct gcgacgcctg ccctggcacc
    10861 gctacggggg cgcccgttga cgcccatggg tgtccgacgc ccgtaaccgg cgacttcgat
    10921 cgcgacggcg acgtggactt ggaggatttc ggtcatttcc aggcctgtct atcaggcctg
    10981 tccgttcctc aggagaatcc ggattgtcag aatgccaatc tcgacggcga cagcgacgtc
    11041 gacaaggcgg
//
LOCUS       DAOTPW010000189        11010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5598_length_11010_cov_4.761296, whole genome shotgun sequence.
ACCESSION   DAOTPW010000189 DAOTPW010000000
VERSION     DAOTPW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5598_length_11010_cov_4.761296"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="P5316_15755"
     CDS             <1..742
                     /locus_tag="P5316_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008313755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR86464.1"
                     /translation="PDAIVHLAAVAHAGKSNKDPFSTFDHSLRTLENALDIARATKLH
                     IRHFVYLSSSMVYGNFKTPTVDEDTVCEPLGIYGALKFAGEKIVIAYNQVFNLPYTIV
                     RPSALYGERCISRRVSQIFMENAARGLPITVNGDGSDRLDFTYIADLVSGIVNVLENE
                     NALNQTFNLTYGQSRSIAELAGIVAGHFPGIKVEYTPKDNLMPDRGTLCVDKAKRLIG
                     YQPRYPLELGMAEYARWYKELFAPACAA"
     gene            839..1573
                     /locus_tag="P5316_15760"
     CDS             839..1573
                     /locus_tag="P5316_15760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR86465.1"
                     /translation="MSGVTDHLKPDAWLAERLGRETYRLTVDEALLDPAGEAERDALL
                     AILNRPAFVYTKVSPTFMEGLRFLQRHDFRLVDTNVIFEKKAYPGRQETEDSGVRLAK
                     PSDRDATVELARSSFRYSRFHLDPQIPRETADRIKADWVAAYFAGTRGHQMVVIQVDE
                     EVAGFLQLLRAADGSLVIDLIAVDERYRRRGLGAEMIAFVESRTRAGELIRVGTQIAN
                     IASVRLYESAGFRMTEAQYVFHYHHA"
     gene            1583..2593
                     /locus_tag="P5316_15765"
     CDS             1583..2593
                     /locus_tag="P5316_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRR86466.1"
                     /translation="MRIGELDLNQRVMVAAEIGNNHEGSYSLAQEMILRAAQAGVDAV
                     KFQTIVPERLVSPGEEKRLAMLRRFQLSYEQFARLQEVATNAGVLFLSTPFDLQSAGF
                     LAPLVPAFKIASGDNNFFPLIDVIARTGKPIILSAGLADLEDVRRTKGFIERIWTELG
                     VDPGLAVLHCVVSYPTPAKDANLLAIRKLASLGVTVGYSDHTVGIEAAVLSVACGARI
                     IEKHFTISKTYSDFRDHRLSADPSDMADLVRRVRLAEEMLGSQEKRMLEAEQPLALAT
                     RRSIVAARDLPAGTVLKWEHLDWLRPGGGLAPGRESELLGRRLACPVAKGKPLMPDHL
                     QP"
     gene            complement(2689..3996)
                     /locus_tag="P5316_15770"
     CDS             complement(2689..3996)
                     /locus_tag="P5316_15770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR86467.1"
                     /translation="MRSGKVSGCFDPGLIGYNARCHKHGRAARKAGRRPIGVDETMAS
                     TPSVAIIIATMNRRDVVLFTLARLHEPNRIPGDYEIAVCVNPSSDNTVKAIRERFPDV
                     RVISLERNLGSCAKAIGVAETRSEYVVFLDDDSHPWPDAIARMFEKFRADKRLGAAAF
                     TVHLPDGRQECSALPGVFVGCGVGFRRAALDAVGGLDGSFFMQAEEYDLSFRLAAGGW
                     KVKTFTDLGVDHLKTPTARLSGRTVRYDTRNNRIVIARYLPDEYEEVYRQDWMQRYRW
                     IGEANGHLRDYWRGRLAGAFRATRDRRTYARWRLSSTAFESLFRVKHIASQMQRLADS
                     GVRRIILADLGKNIYPFVYGAQSAGLEVLCIADDRFARPGRRYRGIPVVETAESLMMK
                     GDAIVVSNASAVHAESRHRALEKATDLPIHRWFGYDKPLPEGV"
     gene            4094..6808
                     /locus_tag="P5316_15775"
     CDS             4094..6808
                     /locus_tag="P5316_15775"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRR86468.1"
                     /translation="MKTCPIARVLLMTAISHGVTGWHVTRAADVTSCVIVQERKPVVE
                     ILVAGENPVLDAAVKDLTKYVELISGAELKVTRGTEDLPGPTLHIGETSLYDDTAEAR
                     KHIKLDGFVIARLGENLIVAGNIPQGTANGVYTILQDQFGVRWYYAGPLWEVVPKATS
                     LAINVKPNAPGGAYLENPSFYGRQMWGRMPSDDFGRRMRMTHEGVKLPYHGTGHALNQ
                     VVNPQKYGDHPEYFAFWDGKRHVEHDVHPCFTHPDMFDIFMRYVREGGTNFGVNDNLT
                     ACKCDRCLAVDGKSEPYNGMWNFSESYFQLIARVAAQTAKEFPDRQLGVFAYQLTTTP
                     PKTVEHIGHNVTVVLCEDTAQNFDPKYKEIDQRIAAEWVRKAGAVRFYDYVGINYWTP
                     RYFPHILADQMRHIARIGVQGYGTHHVSMIDTSMPMYYLLYQLLWNAELDPDAVIDRM
                     ITDLYGNAAGPVGKFYRHWEDRWQRQTKGRWLGGIDDLRGEMQIYAWEDFVRGRELLE
                     EAAKLADDDTVRRRVDFLRQRYDFTYAGAEAHHVSMNAIRWRPTDNYEDAITQSNAVV
                     EAWRKWVRRFHIAQALPDLPVSGWPGMTGYVRMWGLKQQMRDAALAPLVRWTCANEEK
                     NEPARLRLVEQMLAGVGVLNREHIERLITYEANAARRKPRADGLRAADVPRTPDMIPM
                     RAGPDDWPGIPYVDELPWVFHARPAEPKIGKYDEPVRYYYVDPPVPDDLSVTWQAAWD
                     RQRLYLRVVVKDQTHTQNQPAADMWKEDSLQIAFNPDRSNFELKAPSWDYIWGGYKRN
                     ESEIGISLKDKTVQKHIWRSPELPQGVRAEDLVEVSVARHGGGRTIYEAAVQWQLLPG
                     FEPKPRKSLGICLVVNDLDKDERHSAEYGSGVIRAKRPTEFAAIRLAE"
     gene            7323..8750
                     /locus_tag="P5316_15780"
     CDS             7323..8750
                     /locus_tag="P5316_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86469.1"
                     /translation="MAEKIRGFVGFFRATVEQTATELEQKATAREERQAATRFRVRLS
                     DQCRTAASQSDPAEAMLDLWALAYRLVDFLSVGEGRQLFGTSQPVALQAAEEIRAAID
                     EIARKSLSESVFEKTRQDVVAYVKQNPMQEGFLVRPAESFSDVPQGEQVLQQLVAIPF
                     APIAALGGVSRTPESVHAVARSMDRFTDTVEDFPANARWQLQLLGTSFGQTPQFTDLM
                     DNLRQFSDSSARMADNTTEFVDVAKKMPHELRTEAENLLDRIDASQPELRTTLGEAKE
                     TVAMVSSASNHVRDTVVEARVTLDNVQKASQALEKAAQAVQATAQEILKFVPASMKDE
                     TGRIIGDSGQSPENTDAAVEKPASSPVQGNPDDSVETHDARFSFQAVTQSAIELGETS
                     DKLRALLTDFRSFLDAKTFSSEVVAVDTQWRSAADFTSMRIRDIVDHVAKRTAQLLLL
                     FFVLLVLYRLLSRQQNRVKSAARPR"
     gene            complement(8725..10017)
                     /locus_tag="P5316_15785"
     CDS             complement(8725..10017)
                     /locus_tag="P5316_15785"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR86470.1"
                     /translation="MFRGLTLETVRLGLKNLYLHKLRSFLTTLGIICGVGAVICMLSI
                     GEGASEAEMALIRLLGTQNIIVQSVRPQESGVVGSTRTYVLEYGLTRADLSLIRATIP
                     NVNRVVPLREVAYQVRRGSAQLPAAVFGTEPAFFDILHIKIARGRPLQLLDEAEAAKV
                     CVIGDELRARLFAHEDPIGETVYVDRPATPLTPYKVVGILERTQTAGQPAKGLGERDI
                     NSEVFIPLSTADRLYGDLLIRVTSGSYERSRVTFSDFYVQVDDIENVISVSEMVKQAL
                     SHMHNKADYTVKVPLERLRLAESEKRRRQITMGCIAGISLLVGGIGIMNIMLATVTER
                     TREIGIRRALGAKRRHIVMQFLIESLMLSTLGGMIGIVCGSAGAHLITNWVGWPTVIH
                     NWTILCSFGLALAVGLFFGIYPAANAARLDPIEALRQT"
     gene            complement(10021..10806)
                     /locus_tag="P5316_15790"
     CDS             complement(10021..10806)
                     /locus_tag="P5316_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR86471.1"
                     /translation="MPSAVTTDQHRPAARGGRGARVLAHLDGVVKIYRKVGTSVEVPA
                     LRGITLDFAEGEYVAICGQSGSGKSTLLNLLGCLDRPSSGQYVLGGVDVSELDDDALS
                     DIRSRRLGFVFQSFNLIPQLTVLENLEVPLFYQAVTPQLRRERALDLAHRMGLSDRIH
                     HRPMELSGGQQQRVAIARALMNDPLLILADEPTGNLDTATGEVILALFDQLHQAGRTI
                     LMVTHEADVAARCRRVITLRDGLVVSDVSNGRRMPGPAVVGRA"
BASE COUNT         2236 a         3460 c         3288 g         2026 t
ORIGIN      
        1 cccggacgcc atcgtccact tggcggcggt ggcccatgcc ggcaagtcca acaaggaccc
       61 cttttccacg ttcgatcaca gcctgcggac gctggaaaac gccctggaca tcgcccgggc
      121 cacaaagctg catatacgcc acttcgtcta tctctcatcg agcatggtct acggcaactt
      181 caagaccccc acggtcgatg aggacaccgt ctgcgagccc ctgggtatct acggagccct
      241 gaaattcgcc ggcgagaaaa tcgttatcgc ctacaatcag gtgttcaacc tcccgtatac
      301 catcgttcgc ccctcggccc tctacggcga gcgctgtatc agtcgccgcg tcagccagat
      361 cttcatggaa aacgccgccc ggggcctgcc catcaccgtc aacggcgatg gttcggatcg
      421 actggacttc acctacatcg cagacctggt ctcgggtatc gtgaacgtgt tggagaacga
      481 gaacgcactg aaccagacgt tcaatctgac ttacgggcag tcgcggtcga tcgccgagtt
      541 ggccgggatc gtggccgggc actttcccgg tattaaggtc gagtatacgc cgaaggataa
      601 tctgatgccc gaccgcggta ccctttgcgt ggacaaggcg aagcgattga tcggttacca
      661 gcctcggtat ccgttggagc tcggcatggc cgagtacgcc cgctggtaca aggagctgtt
      721 cgccccggcc tgtgcggcct gagcatctgg cgcggaagat tgaaacgcta ccgggcggct
      781 gcgcgcagcg gtaccgaaaa cgacggccac gttggcccga tggacatgga ggtctgccat
      841 gagcggcgtt accgatcacc tcaagccgga tgcctggttg gccgagaggc tcggccgtga
      901 aacgtaccgt ctgaccgtcg atgaggcctt gctcgacccg gccggcgagg ctgaacggga
      961 cgctctgctg gcgattctca ataggcccgc gttcgtctac acgaaggtct cgccgacttt
     1021 catggaaggc ctgcgttttc tccagcgaca tgattttcgc ctcgtggaca ccaacgtcat
     1081 cttcgagaag aaggcttatc cgggtcgcca ggaaacggaa gactcagggg ttcgactggc
     1141 caagccgtcg gatcgcgacg ccaccgtcga gttggcccgg agcagcttca gatactcgcg
     1201 attccacctc gacccccaga tcccgaggga aaccgccgac cggatcaagg ccgattgggt
     1261 ggccgcatac ttcgccggca cccggggaca tcagatggtt gtcatccagg tcgacgaaga
     1321 ggtcgcgggc ttcctgcagt tgctccgagc cgccgacgga tcgctggtca tcgatctgat
     1381 cgccgtcgac gaacgctatc ggcgccgggg actcggcgcg gagatgatcg cgtttgtgga
     1441 gtcgagaacc cgcgcgggcg aactgattcg cgttggcacg cagatagcca atatcgcttc
     1501 cgtaaggctc tacgagagcg ccggtttccg aatgaccgag gcccaatacg tatttcacta
     1561 tcaccatgct tgaggccgca gcatgcgaat aggcgagctc gatctgaatc agcgagtgat
     1621 ggtggcggcc gagatcggca acaaccacga aggctcttat tcgcttgcgc aagagatgat
     1681 cctccgggcc gcccaggcgg gggtcgacgc ggtcaagttc cagaccatcg tgccggaacg
     1741 actcgtttca cccggcgaag agaaacgtct ggcaatgcta cgacgcttcc agctatcgta
     1801 tgagcagttt gcccgattgc aggaagtggc gacgaatgcc ggcgtcctct tcctctcaac
     1861 gccgtttgat ctgcagagcg ccgggtttct tgcgccgctc gtccccgcgt tcaagatcgc
     1921 ctccggcgac aacaacttct tcccgctgat cgacgtaatc gcccggacgg gcaagccgat
     1981 catcctttcg gccggcttgg cggacctgga ggatgtgcgt cggacaaaag gcttcatcga
     2041 gcggatctgg acggagctgg gcgttgatcc gggcctggcc gtcctgcatt gcgtcgtcag
     2101 ttacccgaca cccgccaagg atgccaattt gctggccatt cggaaactgg cctcgctggg
     2161 cgtgacggtg gggtattcgg atcataccgt cggcattgag gcggccgtgc tctcggtggc
     2221 gtgcggtgct cgcatcattg aaaagcattt caccatctcc aagacatact ccgacttccg
     2281 cgatcaccga ttgtcggccg acccgtcgga tatggccgac ctggtccgcc gtgtacggtt
     2341 ggccgaggag atgctcggtt cgcaggaaaa gcgcatgctc gaagccgagc agcccctggc
     2401 ccttgcgacg cgacgctcca tcgtggccgc acgggatctt ccggccggaa cggtgctgaa
     2461 atgggagcat ctggactggc tgcgaccggg cgggggcctg gctccgggac gcgaaagcga
     2521 actcctgggc aggcgactgg cttgtccggt ggccaagggc aaacccctca tgcctgacca
     2581 tcttcaaccc tgaacctgag gccgaccgcc tttttgcgga ccgcaaccca ggtgctctgc
     2641 gatccagaaa cacacgcgca aaatgcacac cagttgcgac ggaagagact aaacgccctc
     2701 gggcaagggc ttgtcatatc cgaaccacct gtgaatcggc agatcggtcg ccttctccaa
     2761 ggcgcgatgg cggctctccg cgtgaacggc tgatgcgttg gaaacgacga tggcatcgcc
     2821 tttcatcatc agcgactcgg ccgtctcgac gaccggaatg cctcgataac gacgcccggg
     2881 ccgcgcgaaa cggtcatcgg cgatgcacag gacctcaagc cctgccgact gagcgccgta
     2941 aacgaacgga tagatgttct tgcccaggtc cgcgagaatg atgcgccgga cgccgctgtc
     3001 ggccagccgc tgcatctggc tcgcgatgtg cttgacacga aacaatgatt cgaacgccgt
     3061 cgaagacaac cgccaacgag cgtaggtccg gcgatcgcgc gtcgcgcgaa acgctccggc
     3121 aagccggccc ctccagtaat cccggagatg cccgttggct tcgccgatcc atcgataccg
     3181 ctgcatccag tcctgccggt aaacctcctc gtactcatcg ggcaggtaac gggcaataac
     3241 gattcggttg tttcgggtat cgtaccgcac cgttcggccg ctgaggcggg cagtgggcgt
     3301 cttgaggtga tcgacaccca gatccgtgaa ggtcttgacc ttccagccgc ctgccgccag
     3361 gcgaaagctc aggtcatatt cctcggcttg catgaaaaac gagccgtcga gcccccccac
     3421 ggcatccagg gcagcccgtc gaaaccccac gccacacccc acaaaaacgc cgggcagggc
     3481 gctgcactcc tgccggccgt cgggcaggtg aacggtgaac gccgccgctc ccaaccgctt
     3541 gtcggcccgg aacttctcga acatccgggc aatcgcgtcc ggccagggat gagaatcgtc
     3601 atcaagaaag acaacgtatt ccgatcgggt ctccgcaacg ccgatggcct tggcgcaact
     3661 gccgagattg cgctcaagac tgatcactcg gacgtcagga aatcgctccc ggatcgcctt
     3721 gaccgtgttg tcgctcgacg gattgacgca gacggcgatt tcgtagtcgc cgggaatacg
     3781 attcggttcg tgcaatcggg ccagcgtgaa cagcaccacg tcccggcggt tcatcgtggc
     3841 aatgatgatc gcgactgacg gcgtgctggc catcgtttcg tccactccga tcgggcgacg
     3901 ccctgccttc ctcgcggcac gcccgtgctt gtggcagcgg gcattatagc cgatcaggcc
     3961 cgggtcgaaa cagccgctga ctttgcccga tcgcaacccc cggttaacat tacctgatca
     4021 ggaatccgtg tcggctgagg atcctcggcc gaccgtacac ggcgttcggg gcctgaacag
     4081 gcggagcttt gccatgaaaa cgtgccctat cgcacgggtg ttgctgatga ccgcgatcag
     4141 ccacggggta acgggttggc acgtgacccg cgccgcggat gtgacatcct gcgtgatcgt
     4201 ccaggaacgc aagccagtcg ttgaaatcct cgtcgccggt gagaacccgg tgctggatgc
     4261 ggcagtcaag gatctgacga agtacgtcga gctgattagc ggtgcggaac tcaaagtcac
     4321 caggggaacc gaagatctgc ccggtccgac gctgcacatc ggcgagactt cactatacga
     4381 cgacacggcc gaagcccgaa agcatatcaa gctcgatggt ttcgtgatcg cccggctggg
     4441 cgagaacctg atcgtcgcgg gcaacattcc ccagggtacg gccaacggcg tctacacaat
     4501 cctgcaggac cagtttggcg tccgctggta ttacgcgggt ccgctgtggg aggtcgtgcc
     4561 gaaggcgaca tcgcttgcaa tcaatgtcaa accgaatgcc ccaggcggag cctatctgga
     4621 aaacccctca ttctatggac gtcagatgtg gggcaggatg ccctccgacg acttcggacg
     4681 acgaatgcgg atgacgcacg aaggcgtcaa gctcccctat cacgggacgg gtcatgcgct
     4741 gaatcaggtg gtgaacccgc agaaatacgg tgatcatcct gagtactttg ctttctggga
     4801 cggcaagcgg cacgtcgaac acgacgtaca cccctgtttc acccaccccg acatgttcga
     4861 tatcttcatg agatacgttc gagaaggcgg aaccaatttc ggcgtcaacg acaacctgac
     4921 ggcgtgcaag tgcgatcgct gtctggccgt cgacggcaag tccgagccgt acaacggcat
     4981 gtggaacttc tccgaaagct acttccagct tatcgccagg gtcgcggcac aaacggccaa
     5041 ggagttcccc gaccgccaac tcggcgtctt cgcctaccag ttgaccacca ccccgccgaa
     5101 gacggtggag cacatcggtc ataacgtcac cgtcgttctc tgcgaggata cggcacagaa
     5161 cttcgacccc aaatacaagg agatcgacca acgaatcgcc gccgagtggg tgagaaaagc
     5221 gggagcggtg cgattctacg actacgtcgg catcaactac tggacacctc gctatttccc
     5281 gcacatcctg gccgaccaga tgaggcacat cgcccggatc ggcgtccagg gctatgggac
     5341 acaccacgtc tcgatgattg acacctcaat gccaatgtac tacttgctgt atcagttgct
     5401 ctggaacgcc gaactcgatc ccgacgcggt gatcgaccgg atgataaccg atctctacgg
     5461 caacgcggcc ggcccggtgg gaaagttcta cagacactgg gaagaccgct ggcaacgtca
     5521 gaccaagggg cgatggcttg gcgggatcga cgatcttcgc ggggaaatgc agatctatgc
     5581 ctgggaggat tttgttcgcg gccgtgaact gctcgaggaa gccgccaaac tcgctgacga
     5641 cgatacggtc cgccggcgag tcgacttcct gcgtcagcga tacgacttca cgtatgccgg
     5701 cgccgaggcc catcacgtgt cgatgaacgc catcaggtgg cgaccgacgg acaactacga
     5761 ggacgccatc actcagtcga atgccgtcgt cgaggcgtgg cgcaagtggg tcaggcggtt
     5821 tcacatagcg caggcgcttc ccgatctgcc ggtctccggc tggccgggaa tgaccggtta
     5881 tgttcggatg tggggtctca agcagcagat gcgcgatgcc gccctcgccc cgctggttcg
     5941 ctggacctgc gccaatgaag aaaaaaacga gccggcacgc ctgaggctcg tcgagcagat
     6001 gctcgccggc gtgggcgtcc ttaaccggga gcacatcgag cgcctgatca cctatgaggc
     6061 caatgcagca cgtcgcaagc cgcgggctga tgggctgcgg gcggccgatg taccgcgtac
     6121 tcccgacatg atcccgatgc gcgccgggcc ggacgactgg cccggcatcc cctatgttga
     6181 cgaactgccc tgggtgttcc acgcccggcc cgctgagccc aaaatcggca agtacgacga
     6241 gccggtacgg tactactatg tcgatcctcc cgtgccggat gacctgtccg taacctggca
     6301 ggccgcatgg gaccggcagc ggttgtacct gcgcgtggtg gtcaaggatc agactcacac
     6361 ccagaaccag ccggcggcgg atatgtggaa ggaggacagc cttcagatcg ctttcaatcc
     6421 ggatcgctcg aacttcgagt tgaaagcccc ttcctgggac tacatctggg gcggctacaa
     6481 gcgcaatgaa tccgagatcg gcatctcgct gaaagacaaa acggttcaaa agcacatttg
     6541 gcgttccccc gaactgccgc aaggggtccg ggcggaggac ctcgtcgagg tttccgtggc
     6601 ccggcacggc ggcggacgca caatctacga agcagccgtc caatggcaac tgctgcccgg
     6661 cttcgagccc aagccgcgaa agagcctcgg catctgcttg gtggtcaatg acctggacaa
     6721 agacgaacgc catagtgccg aatacggcag cggagtgatc cgcgccaagc gcccgacgga
     6781 gttcgcggcc atccggttgg ccgaataata actcggtcgg caccccgtcg catcccagtc
     6841 ggcggtcgca agggccccgc attcccgacg gcaggtttcg tgctgcttgt cgcggccacc
     6901 aagccttggt cagccggaag gttgccgatg cgtcgcagca tcgccacagg cgggtttctt
     6961 tcaccgatat ctgtcccgcc ctatgatgca tattcacaaa ccgccgagca ggacttccgg
     7021 cgcgaaaacg cgtccgcggt gccgtgttgc tgcttgtcgc caacggccct tgcaaggcgg
     7081 tcccgtaaac ggaccagagg tttcactgtc ccccaacgga gatcgcgatc atgaaaccat
     7141 cctatgagca aacccgtcga tgtgcatcag gtcggcaagc cggccgcggt cttgccgcat
     7201 gtgaactcgc gttggccgct tggctactga tggtgccgct atgcggctgc aatggccaac
     7261 cggttcagac gcgacctcag atcgctccgc agcaatatgc gccgacggcg gataaagacg
     7321 aaatggctga gaaaatccgc ggcttcgtgg gcttttttag ggcaacggta gagcaaactg
     7381 ctaccgaact cgaacagaaa gcaacagccc gggaagaacg acaggccgca acccggtttc
     7441 gcgttcgact ttccgatcag tgccgaaccg cggccagtca atcggatccc gccgaagcga
     7501 tgctcgatct ctgggcactg gcttatcgcc tcgtcgattt cctctccgtg ggcgagggta
     7561 gacaactgtt cggcacatct caacctgtcg cccttcaagc cgcggaagag atccgcgcgg
     7621 ccatcgacga aatcgcccgc aagagccttt ccgaaagcgt atttgagaaa acccgacagg
     7681 acgtggtggc atacgtgaaa cagaatccta tgcaggaggg cttcttggtg cggcccgcgg
     7741 agagtttcag cgatgttcct cagggcgaac aggtgcttca gcaactggtt gccatcccct
     7801 tcgccccgat tgcagctctc ggaggcgtga gcaggacgcc ggagtcggtc cacgcggtgg
     7861 cacgttccat ggaccgcttt accgacaccg ttgaagattt tccggccaac gcgcgctggc
     7921 aacttcagct actgggaacg agtttcggac aaacgccaca gttcaccgat ctgatggaca
     7981 acctccggca gttctccgac agctccgcgc ggatggcgga caacaccacc gagtttgtgg
     8041 acgtcgcaaa gaaaatgccg cacgagcttc gaaccgaggc cgaaaacctg ctcgaccgta
     8101 ttgatgcttc tcaacctgag cttcgcacga cgttgggcga agcgaaagag accgtggcaa
     8161 tggtgtcatc tgccagcaat cacgttcgcg ataccgttgt agaagccaga gtcacccttg
     8221 ataacgtcca gaaggcgtct caggcgctcg agaaggcggc tcaggcggtg caggccaccg
     8281 cacaggagat actcaagttc gtgcccgcat ccatgaaaga tgagacgggt cggatcattg
     8341 gcgactcagg ccaatcgccg gagaacaccg acgctgctgt ggagaaaccg gcgtccagcc
     8401 ccgttcaggg gaacccggat gattctgtcg agacgcacga cgcacgattc agcttccagg
     8461 cggtcactca gagtgcaatc gaactcggcg aaacctcgga caagctgcga gccttgctga
     8521 ccgatttccg atcttttctc gatgcgaaga ccttctccag cgaggtcgtc gcggttgaca
     8581 cccagtggcg tagcgcggcc gatttcacga gtatgaggat ccgcgacatc gtcgatcacg
     8641 tcgccaaacg aaccgcccag ttgctgctcc tgttctttgt gctgctggtc ctctatcgcc
     8701 tgctcagccg tcagcagaac cgcgtcaagt ctgccgcaag gcctcgatag gatccagccg
     8761 ggccgcgttg gcggcgggat agatgccgaa aaacaatccg acggccaaag ccaggccgaa
     8821 gctgcagagg atagtccagt tgtggatcac cgtcggccag cccacccagt tggtgatcag
     8881 atgagctccg gcggagccgc agacgattcc gatcatgccg ccgagggtgc tgagcatcag
     8941 tgattcaatg agaaactgca tcacgatatg ccggcgcttg gcgcccagcg cccgccgaat
     9001 gccgatctct cgcgtccgct cggtcacggt cgctaacatg atgttcatga tgccgatgcc
     9061 gccgaccaac agggagatgc cggcaatgca acccatggtg atctgccgcc ggcgcttctc
     9121 gctttcagcc aacctgagcc gctccagcgg caccttcacc gtgtaatcgg ccttgttgtg
     9181 catgtgggac aaggcctgct tgaccatctc ggatacggaa atcacgttct cgatgtcgtc
     9241 gacctgaaca taaaagtcgc tgaatgtcac ccggcttctc tcgtagctgc cgctcgtgac
     9301 gcgaatcagc agatcgccgt ataacctgtc ggcggtgctg agcgggatga agacctccga
     9361 attgatgtct cgttcgccca gccccttggc cggttggccg gcggtttggg tccgctccag
     9421 gataccgacc accttgtagg gggtcagagg cgtggccggt cggtcaacat agacggtttc
     9481 cccgatggga tcctcgtgag cgaacagcct ggctctcagc tcgtccccga tgacgcacac
     9541 cttcgcagcc tccgcttcat cgagcaactg gaggggccgg ccacgggcga tcttgatgtg
     9601 cagaatgtcg aaaaaagccg gctccgtacc gaacaccgcc gcgggtagct gcgcgctgcc
     9661 tcttcgcacc tgataggcga cttcgcgtag aggaacaacg cgattgacgt tcggaatcgt
     9721 cgcccgaatc agcgacagat ccgcgcgggt caggccgtac tccaacacgt atgtccgtgt
     9781 cgagccgacg acgccgctct cctggggacg aacggattgg acgatgatgt tctgcgtgcc
     9841 gagcaggcga atcagcgcca tctctgcttc ggatgctccc tcgccaatgg acaacatgca
     9901 gatgactgca ccaaccccgc agatgatgcc cagcgtggtg agaaacgagc gcaatttgtg
     9961 caggtaaagg ttcttgagcc ccagtcggac ggtctcgagc gtcagtccgc ggaacatggc
    10021 tcaggctctc cccacgacgg ccggtccagg catccgtcgg ccgttgctca cgtcgctgac
    10081 caccaagccg tcccgaaggg tgataacccg tcggcaacgc gccgcaacgt cggcctcgtg
    10141 agtgaccatc agaatcgtcc gcccggcttg atgaagctga tcgaacagag caaggatgac
    10201 ctcgcccgtg gcggtatcca gattccccgt gggctcgtcg gcgagaatca ggagcggatc
    10261 gttcatcagg gcccgggcaa tagccacacg ctgctgctgg cctccggaca gttccatcgg
    10321 acggtgatga atgcgatccg acaggcccat ccgatgagcc agatcaaggg ccctctccct
    10381 gcgcagttgc ggggttaccg cctggtagaa aagcggaacc tcaaggttct ccaggacggt
    10441 gagctgtggt atgaggttga aactctgaaa gacaaatccc aaccggcggc tccggatgtc
    10501 cgacagggcg tcgtcgtcga gctcggacac atctacgccg ccaagcacgt actgcccgct
    10561 gctcggccga tcgaggcaac cgagcagatt cagaagcgta ctcttgcccg acccgctctg
    10621 gccgcaaatg gccacgtact cgccctcggc aaaatcaagc gtaatgcctc tgagcgcggg
    10681 cacctcgact gaagtgccga ctttgcgata aatcttgacc acgccgtcca gatgggcgag
    10741 gactctggct cccctgccgc ctcgagctgc cgggcgatgc tgatctgtcg taacggcact
    10801 cggcatgacc atcggtcgct actgtaccat cagaacctta cggccggacg tctgcgcttc
    10861 ttgcgcgcgt gtttctggat cgcagtgcgc ttgggttgcg gcccgcaaaa tgccggtccg
    10921 ccttgggatc ccggcctcag ttcatcgcag gcgccgaggc cggcggagcc ccccttctct
    10981 tcgcccccgc ccctttcccg gccttctcct
//
LOCUS       DAOTPW010000190        10984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5613_length_10984_cov_5.563089, whole genome shotgun sequence.
ACCESSION   DAOTPW010000190 DAOTPW010000000
VERSION     DAOTPW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5613_length_10984_cov_5.563089"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            531..803
                     /locus_tag="P5316_15795"
     CDS             531..803
                     /locus_tag="P5316_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HRR86472.1"
                     /translation="MAQAYVDPSELRRFAQDLNRFNGELHSLIAGLHARMLSLEKTWR
                     DQEQRKFAEEFEQTMRVLNAFLKLSERHVTFLLKKAGYIEDYLQQH"
     gene            813..1871
                     /locus_tag="P5316_15800"
     CDS             813..1871
                     /locus_tag="P5316_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86473.1"
                     /translation="MSQAARVESIEKLKDLRVALCLFAETARTGLLEGDSEIQRVGLW
                     LKNEQLRYWKSQIVSRSELLTRAKIALNQKKLTKTPLGGHYSCVDEEKAVQLARRRLE
                     EAETKLANVQKWNRRLDEEVFEYKGQVQALGRMVDSELPAAIARLDRMIESLESYVSL
                     KAASGEEVDLHAAFASVARTSSLAELLDSPWPESREAAYAELRRLSPPAEIRQQAPVG
                     PIPKPLAGRIVSQADRDGIASLGITRTAPSPGSRLVFATGCETWPHLYFERSEPAGPE
                     DSGWYAGPVEGDPPDRFQAVQIGELIAQGPQWAPILDLPVGTLLVLRAESLEVLLDDR
                     DAILWPSPSQSADKRRGT"
     gene            1868..2080
                     /locus_tag="P5316_15805"
     CDS             1868..2080
                     /locus_tag="P5316_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86474.1"
                     /translation="MSLHTGQANMRKAAKDLAARWDEVRTLWRDDVARQFEERYIHSL
                     LREVRVTQEAMAHMSSVIAQIRQDCE"
     gene            2105..6076
                     /locus_tag="P5316_15810"
     CDS             2105..6076
                     /locus_tag="P5316_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRR86475.1"
                     /translation="MSQEAKQYPPDESSSGSDYLDVQRAALRQWIDLSVEAVQAAQAP
                     FEHKYRSASRKAQQRYDQARDKVEHYLQTRLDHACRHYKEQIAQIESLHASDLRTLEE
                     NTASLRSRITLELTRAEERAKSKLDHELWLSESVAQATREQLARDLKEFKEAFPNQEG
                     MISTLREQADWLLCQYGQMRLAETQDPPGHVPESGNPQGTFQQQVERAQEQIAALAEL
                     SWPAWLTGAKLYFLAIFACAAAVGLAALPHMLGWAGWPPFAIAGPAAFGLSLAAVVVV
                     VRLIAPKASARVREAYLPIRATLANARLALDQYSSQFHRRLEEKQAESARKREAEESR
                     AKKKYDMEMAEARKTAESSMKKADEAHSWLKREIEDRHAANRQKADREWKPLIQRLEQ
                     RLERSTTAVRRRYEREMTACQAAYDRARAELQTRCRIGLEHIRAFLDRTAGLDPRALT
                     DWSDPAWQQWRPTSRFSPVIRFGQIEIEMKRLSEDVLRLGNFAADYPANLCVPAVLTL
                     PHHASLLVQTDPDGRDEAIAVLQSVMARLLTSLPPGRVRFTIVDPVGLGRNFAGFMHL
                     ADYQEALVDSRIWTEAAHIEQRLTDLTNHMENVIQKYLRNEFESIDAYNRQAGELAEP
                     YRFLVVADFPAGFNEEAARRLSSIVSSGARCGVYTLIAHDARQQPPIGIQIDDIRPRS
                     ITLLHEGGRFVWQDEVFRQFPLTVDAPPAETTLTRLMHAIGNLARDANRVEVPFDTIA
                     PSPEKMWSSNAAEELRIPIGRTGAVRIQELRLGRGVAQHALIAGKTGSGKSTLLHVMI
                     TNLALWYPPEEVELYLIDFKKGVEFKTYVTNDLPHARAVAIESDREFGLSVLRRIDAE
                     MNRRGDLLRRAGVQDLPAYRQATGRNMPRTLLIVDEFQVFFSEDDKLAQDAAILLDRL
                     VRQGRAFGIHVILGSQTLGGTSGLARSTIGQMAVRIALQCSDADSRLILDDENAAARL
                     LSRPGEAIYNDAGGLITGNSPFQTAWLADDRRDELLERVGLKARERTVDREPLIVFEG
                     NAPSEIAQNRLLARLLAAPNWPVTSAAPQAWLGEAVAIKDPTSVAFRRQSGSNLLMVG
                     QRDEAALAMMASAMVSLAAQQPPRSARFVILDGSPVDAPWAGYLQRVASILPHATRIV
                     EYRELTEAMNELTAELQRRQQGEVTSSDPSMYLIIYGLQRYRPLRRQEDEFSFARTDE
                     DAPPDPGRQLSELLREGPPNGIHTLAWADTPVTLERTFNRQTMREFDNRVLFQMSAAD
                     SSNLIDSPAANRLGLYRALLHSEELGLLEQFRPYAMPQSNWLEYVENRFRFMSSAAGH
                     EIV"
     gene            complement(6276..7856)
                     /locus_tag="P5316_15815"
     CDS             complement(6276..7856)
                     /locus_tag="P5316_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86476.1"
                     /translation="MRCGKKQTWPFFLVCLIVATVVGPVLCGAAQDGSRAIDVGSRKQ
                     LFVDDRFIASSRGVRLTMNPPAKMNDPVLANYIPWEGDEGASAGWYSSVLKDGEKIRI
                     WGSGKALLPVSLKNGGPVIDLYAYAESDDGVHFTKPDPNRHRFDEKPAEVGKHGRTGG
                     VSVWVDPHGPPSERYKTQAKFYPPNAPAEFHVCASPDGYRWTLLCKPAIEEMDTQSIA
                     FWDERLGKYLLYTRKNPLRNTPKRYRVVRRFESIDLLNWENEVFVMQADEIDNSTYAP
                     PTPQPPVDYYGAAVFKYPEDSPDALYIMLAQAFWHWQRRPVEQRQGGYPDHKFSVEVL
                     APATLDVRLAVSRDGIRFQRVGDRKPFIGLGMEGTFSAKRTWALPNPVRMGDELWIYY
                     CGENRDHDGFVDPAASKYLCGIDRAILRLDGFVSADADYTGGEIVTPLITFAGGRLEL
                     NFDGGAGGSVCVEFLDENDKPIEGYSGAAAEPLYGNSVRLPVRWGDKTDVSSLAGKPI
                     RIRFRMRDCKLYAFQFVE"
     gene            8382..9914
                     /locus_tag="P5316_15820"
     CDS             8382..9914
                     /locus_tag="P5316_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRR86477.1"
                     /translation="MPTAVEEFAVRVAPSTGEKRRKVLLISPATPDTFWSYKHVLHFI
                     SKKAAFPPLGLLTVAAMLPREWDLKLVDLNTGRLTDEQIEWADYVFVSAMIVQADSAR
                     QIISRCLDKGKTVIAGGPLFTTGHEQFPEVKHFVLGEAENVMADLVADMTAGSLRRFY
                     QCGERPDITHTPAPRWDLIKLKQYALMPVQFSRGCPFNCEFCDIIIMNGRVPRTKTPA
                     QMIRELESLVDVGWRESIFIVDDNFIGNKVKVKEFLRALIAWSRRRGVKFQFTTEASL
                     NVADDPELLRLMVEAGFKKIFVGIETPQEEGLIECAKAQNTQRDLLASVKTIQKAGIE
                     VMGGFILGFDSDKPNIFERQIKFIQEAGVVTAMVGLLQALPGTQLFSRLEKEGRILSQ
                     ATGNNVMAALNFIPKLDRELLIEGYRSLVKRLYSPQMYYRRIRAFLSEYRPQGPRFRL
                     SCRDIKAFLKSLWIMGIWSRGRREYWKFLSRAVLFHWRAFPEAMTLAIIGHHFRRVAA
                     TL"
     gene            complement(10101..10331)
                     /locus_tag="P5316_15825"
     CDS             complement(10101..10331)
                     /locus_tag="P5316_15825"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRR86478.1"
                     /translation="MARRFLAAAERVFAELARMPALGRLREFEDPRLAGLRSCLITPF
                     DSYVAFYLHTATGIDVVRVLHGAQDIEEHLKG"
     gene            complement(10412..10672)
                     /locus_tag="P5316_15830"
     CDS             complement(10412..10672)
                     /locus_tag="P5316_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRR86479.1"
                     /translation="MTSMNISLPDEMRAFVEQQVANGGYSTASEYVRALIREAVRQEE
                     KRKLETTLLQGLNSGTAVPLTDKQWHAIRQAVDKRLGGQEQQ"
BASE COUNT         2191 a         3511 c         3277 g         2005 t
ORIGIN      
        1 ttcccgaagg gcaaacaggc cggtcgtttt acgactggtt cacggtcggc caattcctcc
       61 ttcactgtgt taccccatcg aggccgtttt gacgggcctg tccttgtcag gtaagcccgt
      121 tgaaacgggc tgagaccata gaaaggaaga agaacagcag gcgaccactg accggccgta
      181 tcacggccgg cctgcctttg gcggggaaac cccatgaaga gcgttgatcg cccaacctgg
      241 accggtcggc atgctagccc agacggtcgc gcacccgcgg ctatggatac tcatcattgg
      301 gctccgtcat ctacccgatg caatccctcg ccggctcctg agaattgacc acaccaaaga
      361 cccgtcccgg tcattcactg tcgcaggggt gcctgtggcg gcctgacact tatgtcggtt
      421 tcctgtcgaa atcagacggg tttcaggata gaatcagttg aggcccgcaa ggggccaccg
      481 gctggttccc cgccgtgctg atcggccggt accgacgcaa ggagacgctc atggcccaag
      541 cttatgtgga cccgtccgag ctgaggcgat tcgctcagga cctcaaccgc ttcaacgggg
      601 agcttcacag cctgattgcc ggcctgcatg cccgaatgct cagtctggag aagacctggc
      661 gagaccagga gcagcgcaag ttcgccgaag agttcgagca gaccatgaga gtgctcaacg
      721 ctttcctcaa gctgtcggag cgtcatgtga cgttcctcct gaaaaaagcc gggtacattg
      781 aggattacct tcagcaacac taggacctgc cgatgagcca ggccgcccga gtggaatcga
      841 tcgagaaact gaaagacctg cgcgttgcgt tgtgtctgtt cgccgaaacc gcgcgaacgg
      901 gcttgctcga gggggattcc gaaatccagc gcgtcggcct gtggctcaaa aacgagcagc
      961 ttcgctactg gaagtcacag atcgtcagcc gcagtgagct gctgacccgg gccaagatcg
     1021 ccctgaacca gaagaaactc accaagacgc ccttgggcgg acactactcc tgcgtcgatg
     1081 aagagaaggc cgtgcaactg gcccgtcgac gtctggaaga agccgagacg aaactggcca
     1141 atgtgcaaaa gtggaatcgc cgccttgatg aagaggtctt tgagtacaag gggcaggttc
     1201 aggccctcgg ccggatggtc gatagcgagc tgcccgcagc catcgcacga cttgaccgca
     1261 tgatcgagtc gttggaatcc tacgtctcgc tcaaggccgc aagcggggaa gaggtggacc
     1321 ttcacgccgc ctttgccagt gttgctcgca ccagcagcct cgccgagtta ttggacagtc
     1381 cctggcccga atcccgtgag gccgcctacg ccgaactgcg caggctcagc ccacctgccg
     1441 aaatccggca gcaggctccg gtaggaccga ttcccaagcc actggccggg cgtatcgtca
     1501 gtcaggccga tcgcgacggc atcgcaagcc tcggcatcac ccgcaccgcg ccgtcccccg
     1561 gttcccgact ggtcttcgca acggggtgcg aaacctggcc tcatctctac ttcgagcgga
     1621 gcgaaccggc cggacccgag gacagcggat ggtacgccgg acctgttgaa ggcgaccccc
     1681 cagaccgctt tcaggcggtg caaatcggcg aactcatcgc tcagggaccc cagtgggcac
     1741 cgatcctcga tctgcccgtc gggacactgc tcgtcttgcg ggcggaatcg ttggaggtcc
     1801 tgctggacga ccgcgacgcg atcctctggc ccagcccgtc tcaatctgcc gacaaaagga
     1861 gggggacgtg agccttcaca ccggtcaggc gaacatgagg aaggctgcaa aggacctcgc
     1921 cgctcgctgg gatgaggtgc ggactctgtg gcgagacgat gtcgccaggc agtttgaaga
     1981 acgttatatc cactcgctgt tgcgtgaggt cagggtcacc caggaggcca tggcccatat
     2041 gagttcggtc atcgctcaga tacggcagga ttgcgagtag gagcgcggac cgggagtacg
     2101 gccgatgtcg caggaagcca agcaataccc tcctgatgaa tcgtccagcg ggtccgacta
     2161 cctggacgtg cagcgggccg ccctgcgtca atggattgat ctgtcggtgg aagccgttca
     2221 ggcggcacag gcgccgttcg agcacaaata caggtccgcg tcccggaagg ctcagcaacg
     2281 ctatgaccag gctcgcgaca aggttgagca ctatctgcag acccgcctcg accacgcctg
     2341 ccggcactac aaggaacaaa tcgctcaaat cgaatcgctc cacgcgtcgg atctccggac
     2401 ccttgaggag aacaccgcat cgctgcgatc gcgaatcact ctcgaattga ccagggccga
     2461 ggagcgggcc aagagcaaac tcgatcacga gctgtggttg agcgaaagcg tcgcccaggc
     2521 gaccagggaa caattggcca gggacctcaa ggagttcaag gaggctttcc ccaatcagga
     2581 ggggatgatc tcgacgctgc gcgagcaggc ggattggctg ctttgccagt atggccagat
     2641 gcgtcttgca gaaacgcagg acccgcccgg tcacgtcccc gagtcgggca atccgcaggg
     2701 tacgtttcag caacaggtcg aacgggccca ggaacagatt gccgcccttg ccgaactgag
     2761 ctggcccgct tggctgacgg gtgcaaagct ctacttcctg gcgatcttcg cctgcgctgc
     2821 tgccgtcggc ttggccgctt tgccacatat gctcgggtgg gccggctggc cgccgttcgc
     2881 gatcgccgga ccggcggcgt tcggattatc tctcgcggcc gtcgtcgtcg tggtcaggct
     2941 cattgcgccc aaggcgagcg cccgcgtccg cgaagcctac ctgccgattc gggcaacact
     3001 ggcaaacgcg cgcctggcgc tggatcagta ctcgtcccaa ttccaccgcc gccttgagga
     3061 aaaacaagcc gagtcagctc gcaagcgaga ggccgaagaa agccgcgcaa aaaagaagta
     3121 cgacatggaa atggcggaag cccgcaagac cgccgagtca tcgatgaaaa aggccgatga
     3181 ggctcactcc tggctcaagc gggaaatcga ggatcgccac gccgccaacc gccagaaggc
     3241 cgaccgggaa tggaagccgc tcatccagcg gctggaacag cgtctggagc gttcgacgac
     3301 cgcggttcgc cgccggtacg aacgcgagat gaccgcctgc caggccgctt acgatcgggc
     3361 tcgagctgaa ctccagaccc gctgtcgcat cggtctggag cacattcgag cttttctgga
     3421 tcggaccgcc ggccttgatc cccgcgctct gacggattgg agtgaccccg cctggcagca
     3481 atggcgacca acgagtcgtt tcagtccggt cattcgcttc gggcagatcg aaatcgagat
     3541 gaagcgtctt tccgaggacg tcctgaggct gggcaatttc gccgccgatt accccgccaa
     3601 cttgtgcgtg ccggccgtgc tgacactgcc gcatcacgcc tcgctgctgg tccagaccga
     3661 tccggacggt cgtgacgagg ccatcgccgt attgcagtcg gtcatggccc ggctgctgac
     3721 ttccctgcct cccgggcggg tgcgattcac catcgtcgat cccgtgggac tgggccgcaa
     3781 cttcgccggc ttcatgcact tggcggatta ccaggaggcc ttggtcgact cgcgcatctg
     3841 gaccgaagcc gcccacatcg agcaacgcct gaccgacctg acgaaccaca tggagaacgt
     3901 gatccagaag tatctacgca acgaattcga gtcgattgac gcctacaacc gtcaggcggg
     3961 tgagctggcc gagccgtacc gcttcctggt ggtggccgac ttcccggccg gctttaacga
     4021 agaggccgca cggcggctga gcagcattgt cagcagcggg gctcgatgcg gcgtctacac
     4081 gctgattgcc cacgacgctc gccaacagcc gccgatcggg atccagatcg acgacatcag
     4141 accgcgcagc attaccctgc tgcacgaagg gggccgattc gtctggcagg atgaggtgtt
     4201 ccgccagttc cccctgacgg tggacgcgcc gccggccgag accactctga cccgcttgat
     4261 gcacgccatc ggtaacctgg cccgcgacgc caaccgtgtc gaggtgccat tcgacaccat
     4321 cgcgccctcg ccggagaaaa tgtggtcgtc caacgccgcc gaggagctgc gaatcccgat
     4381 cgggcggacc ggcgcggtgc ggattcagga actgcgtctc ggccggggcg tggcccaaca
     4441 tgccctcatc gccggaaaga ccggctcggg caagtcaacg ctgttgcatg tgatgatcac
     4501 gaatctggct ctctggtacc cgccggaaga ggttgagctg tacctgatcg acttcaagaa
     4561 gggcgtggaa ttcaagacct acgtcaccaa cgacctgccg cacgcccgcg ccgtggccat
     4621 cgagagtgat cgtgagttcg gcctcagcgt gcttcgccgt atcgacgccg agatgaaccg
     4681 ccgcggcgac ctgctgcgcc gggcgggcgt gcaggatcta cccgcctatc gccaggccac
     4741 cggccggaat atgccccgaa ccctgttgat cgtggacgag ttccaggttt tcttctccga
     4801 ggacgacaaa ctggcccagg acgccgccat cctgctcgac cgactcgtcc ggcagggccg
     4861 tgcgttcggc atccacgtca ttctcggttc gcagaccctc ggcggaacgt cgggtctggc
     4921 gcgcagcacc atcggccaga tggccgttcg catcgccctg cagtgctcgg atgccgattc
     4981 gcgcttgatc ctcgacgacg aaaacgcggc cgcgcggttg ctttctcgac cgggggaggc
     5041 catctacaac gacgccgggg gcctgattac cggcaacagc ccgttccaga ccgcctggct
     5101 ggctgacgat cgccgcgacg aactgctcga acgtgtcggt cttaaggctc gtgagagaac
     5161 cgtcgaccgc gagccgctca tcgtcttcga gggcaacgct ccttccgaaa tcgcccagaa
     5221 ccgtttgctc gcgcgcctcc tggccgcgcc gaactggccg gtgacctccg ccgcccccca
     5281 agcctggctc ggcgaggcgg tggcgatcaa ggatcccacc tcggtggctt ttcggcgaca
     5341 gagcggctcg aacctgctca tggtcggcca gcgcgacgaa gccgcactgg caatgatggc
     5401 ctcggccatg gtcagcctcg ccgcccagca gccccctcgg tcggcccgtt tcgtcatcct
     5461 cgacggctca cccgtcgatg ccccttgggc cgggtacctg cagcgcgtcg cctcaatcct
     5521 gccgcacgcc acgcggatcg tcgagtaccg cgaactgacc gaggccatga acgagctgac
     5581 cgccgagttg cagcgccgcc agcagggcga ggtgacaagt tcggacccct cgatgtacct
     5641 gatcatctac ggcctgcaac gctatcgccc cttgcgccgc caggaggacg agttcagctt
     5701 cgcgagaacc gatgaggacg cgccgccgga cccgggccga cagctcagcg aactgctgcg
     5761 ggaaggccca ccgaatggaa tccatacgct ggcctgggct gacacgccgg tcactctcga
     5821 acgcaccttc aaccgtcaga ccatgcgaga gttcgataac cgcgtgctct ttcagatgag
     5881 cgccgcagat tccagtaacc tgatcgactc gccggccgcc aatcgcctcg gcctgtaccg
     5941 cgccttgctg cacagcgagg aattggggct ccttgaacag ttccggccct atgccatgcc
     6001 tcagagtaat tggctggagt acgtcgagaa ccgtttccgc ttcatgtctt ccgccgctgg
     6061 gcacgagatc gtgtaagttc ctcggggcaa agacagggta atgtgaaggt cgaaccggca
     6121 atcgaggacc gccagattcc tgaacggtga aacgttcacg cgacggcggc tggttcgaca
     6181 gtccttaccc tggtccacat ggagaaacgg ccccaatcgc gttgagtccg gacaggaccg
     6241 tctcaccttt ggcgtcgggt aggcaaggcg cgctctcact ctacaaactg gaacgcgtac
     6301 agtttgcagt cgcgcatgcg gaatcgaatt ctgatcggct tgccggccag tgaactgacg
     6361 tcggtcttgt cgccccatcg caccggcagg cgaacggagt tgccgtatag cggttcggcg
     6421 gcggcgccgg aatagccctc gatgggcttg tcattctcgt ccaggaactc cacgcacacg
     6481 cttccgcccg ccccgccgtc gaagttcagt tccagccgtc cgccggcaaa ggtgatcagc
     6541 ggggtgacga tttcgccgcc cgtgtaatcg gcgtcggcgg aaacgaaacc gtccagccgc
     6601 agaatcgccc gatcgatgcc gcacagatac ttggacgccg ccggatcgac gaagccgtcg
     6661 tgatcgcggt tctcgccgca ataatagatc cacagttcgt cgcccatacg gaccgggttc
     6721 ggcaaagccc acgttcgctt ggctgagaag gttccctcca tcccgaggcc gatgaacggc
     6781 ttcctgtcgc cgacccgctg aaagcggatc ccgtcgcggc ttacggccag ccggacatcc
     6841 agcgtcgccg gcgccagcac ctcaacgctg aacttgtgat ccgggtatcc accctgccgc
     6901 tgctcgaccg gtcgtcgctg ccagtgccag aaggcctgcg cgagcatgat gtatagggcg
     6961 tccggggaat cctccgggta cttgaacacc gctgcaccgt agtaatccac cggcggctga
     7021 ggagtcggag gcgcgtaggt cgaattgtcg atctcgtccg cctgcatcac gaaaacctcg
     7081 ttctcccagt tcagaagatc gatcgactca aaccggcgga cgacccggta ccgcttgggc
     7141 gtgttccgga gcgggttttt tcgcgtatac aacaggtact tgccaagtcg ttcatcccag
     7201 aaggcgatgc tctgggtatc catctcctcg atggccggct tgcacaacaa cgtccagcga
     7261 tagccgtccg gggaagcaca aacgtggaac tccgccggag cgttgggcgg atagaacttg
     7321 gcttgcgtct tgtatcgctc tgacggcggc ccatgaggat caacccagac gctgacgccg
     7381 ccggtgcgac cgtgtttgcc cacctcggcc ggcttttcgt cgaagcgatg ccggttcgga
     7441 tccggcttcg taaaatgaac cccgtcatcg gattctgcat aggcgtacag atcgatgacc
     7501 ggtcccccgt tcttcaagct gaccggcagg agagccttgc ccgaacccca gatgcggatc
     7561 ttttcgccgt ccttgagcac cgaggaatac caacctgctg atgcgccttc gtcgccctcc
     7621 caaggaatgt agttggcaag aacgggatca ttcatcttcg ccggcgggtt catcgtgagc
     7681 cgcacgcctc ggctggacgc aatgaagcga tcgtcaacga acagttgctt gcgcgaaccc
     7741 acgtctatcg cccgggaacc gtcctgcgcc gccccgcaca aaaccggacc cacaacggtg
     7801 gcgacgatca ggcaaaccag gaagaacggc cacgtctgtt tcttgccgca ccgcattgtt
     7861 cattctccgc agtcaatcag tcctgtgttg aaatggtcaa tcgggcttcg cccccgacgg
     7921 tacacccata ccacacgtcc ggtgccgcgc cgtgttcagc acggtgcgcc gctgtcagcc
     7981 ggctacgcgc ggcagcctta ccgccccgag cctgtaccgg gacctcataa ttgcttccga
     8041 acaggcacac ccacacgtcg gttcaaaacg gggcatgctg ttcctccggt tcttgggaac
     8101 tcccgcgcac cacgaggcac agacagacca tatcctcgcc cgtcggggcg gtcaatgaac
     8161 gaacctgctc ggcgatgacg ggccgagacc ctttaagtcc ttatgcacta aatggttatg
     8221 ttgtgcgaca accaccgggt tcgcttaccc ggggttccgc aaacccatgc gctgcaaaaa
     8281 gtgttcaagc acgcgaagcg tgctataata gtcgatagcc ctgttgccga gtcagtcgcg
     8341 acttggagca gcggcggctc gttcatcgag gcaacaacag aatgcccacc gcagttgagg
     8401 aattcgcagt acgggttgca cccagtaccg gcgagaaaag aagaaaagtc ctgctgatct
     8461 cccctgccac acccgatacg ttctggagct acaagcacgt tctccatttc atctccaaga
     8521 aggcggcgtt tccgcccctt ggactgctga ccgtggcggc catgctcccg cgcgagtggg
     8581 accttaaact cgtggatttg aacaccggcc gtctcaccga cgagcagatc gaatgggccg
     8641 actatgtttt cgtgtccgcg atgatcgtac aggccgattc ggcccggcaa atcatctccc
     8701 ggtgcctcga caaaggaaag acggtcatcg ccggcggacc gctgttcacc accggccacg
     8761 agcagtttcc cgaagtgaag cactttgtcc tcggcgaggc ggaaaacgtc atggccgatc
     8821 ttgtcgcgga catgaccgcg ggatcattga gacggtttta ccaatgcggg gagcggccgg
     8881 acatcacgca tacccccgcg ccgcgatggg acctgatcaa gctcaagcaa tacgccctga
     8941 tgcccgttca gttctcgcgg ggctgcccgt tcaattgcga gttctgcgac atcatcatca
     9001 tgaacgggcg cgtgccacgc accaagactc ccgcgcagat gatccgtgaa ctggagagcc
     9061 tcgtcgatgt cggttggagg gaatccatct tcatcgtcga tgacaacttc atcggcaaca
     9121 aggtcaaggt gaaggagttc ctacgagccc tgatcgcctg gagccggcgg cggggcgtga
     9181 agttccagtt cacgaccgag gcgtcgctga acgtggctga cgatcccgag ctcctgcgtc
     9241 tgatggtcga ggcggggttc aagaagatct tcgtgggcat cgagacgcca caggaagaag
     9301 gacttataga gtgcgcaaag gcccagaaca ctcaacgaga cctcctcgcc tcggtaaaaa
     9361 ccatccagaa ggcaggcatc gaggttatgg gcggtttcat cctcggtttc gacagtgaca
     9421 aaccgaacat atttgagcgg cagatcaagt tcattcagga agccggggtg gtgacggcca
     9481 tggtcggcct gctgcaggcc ctgcctggaa cccagctttt ctcgcgactc gaaaaagagg
     9541 gtcgcatttt gagccaggcc accggcaaca acgtgatggc cgccctcaac ttcatcccga
     9601 agctggaccg agagctgttg atcgaaggct atcgctcgct ggtcaaacga ctgtacagcc
     9661 cccagatgta ctaccgccga atccgggcgt ttctctccga ataccgtccc cagggtcctc
     9721 gtttccgcct gtcgtgtcgc gacatcaagg catttctcaa atcgctgtgg atcatgggca
     9781 tctggagccg cgggcgccgt gagtactgga agttcctgag tcgggcggtg ttgtttcact
     9841 ggcgagcgtt ccccgaggcc atgaccctgg ccatcatcgg acatcacttc cgccgagtgg
     9901 cggcgacgct gtaggaagcg cgctcggtgc ggacacatca actttgtcga acgatctcgc
     9961 gtcgccaagt tcgtggatga tcttgccaaa gagggaattc agcttccgga ccgccctccg
    10021 gatcagacct tcaagatccc ggactggatg tcgacacgca agtaggctta tgcacgagag
    10081 cggcgttgtt cgccgatcac ctaccccttc agatgctcct cgatgtcctg agcaccgtgc
    10141 aggacccgga cgacatcgat gccggtagct gtatgcagat agaacgcaac gtaactatcg
    10201 aaaggcgtga taaggcagga gcgcaggcca gccaatcgcg ggtcctcgaa ttcacgcagg
    10261 cgaccaagcg ctggcatcct ggccaattcg gcgaacacgc gctccgcggc agccagaaac
    10321 ctccgtgcca caagcggatc atcctagcca aggaagacgg catgctcgat caagtcacat
    10381 tcggcctccg gccgtatgag ggcaggaagg ctcactgctg ctcctggccg cccagccgtt
    10441 tgtcgactgc ttggcggatt gcgtgccact gcttgtcggt caggggtacc gccgtgccgc
    10501 tgttcagtcc ttgcaggagc gtcgtttcga gcttgcgttt ctcttcctgt cggactgctt
    10561 cgcggatcaa ggcccggaca tactcactgg cggtgctgta tcccccgttg gccacctgct
    10621 gttcgacaaa ggcccgcatc tcgtcgggca gtgaaatgtt catactcgtc atggcgtaac
    10681 ctttcttgcc accaccgaat gacacacata aattataccg ttctcgacaa gaaatggcaa
    10741 ctcatgttat aaattgaccg ggaccatcct ctttcgccaa gcacgtccga taaaccacgt
    10801 gcccaacccg gtatcctgtt gaagacacac ccgcttggcg gcaggtcgaa aggcattgtg
    10861 cgtgttgcgg ccgcaggttt gtcgacgggc ccgcccagtc gcagaaccgg cccgacgcac
    10921 agagactgtt actgtcgcgg cggtggcacg ttgcggggcg cgatggccca gtccagcagg
    10981 tcga
//
LOCUS       DAOTPW010000191        10963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5623_length_10963_cov_5.606069, whole genome shotgun sequence.
ACCESSION   DAOTPW010000191 DAOTPW010000000
VERSION     DAOTPW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5623_length_10963_cov_5.606069"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="P5316_15835"
     CDS             <1..441
                     /locus_tag="P5316_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86480.1"
                     /translation="CVSSRKHPFLTPQPLRADIAIERASPGCPARVSPPAEKPRLEAL
                     FKGIHWERTDMRLGLEKKKHSLLRTLACLATGGMVLSVIGCEGEVGGVMVDALEAALQ
                     AAIPGLMDLLRADVVNDGSGTGTGGGSLPTVMHEAVQMVRLMLA"
     gene            complement(855..2231)
                     /locus_tag="P5316_15840"
     CDS             complement(855..2231)
                     /locus_tag="P5316_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRR86481.1"
                     /translation="MKALRTHLIILVPLVGLTPGCSPDSGGTGILDGLFNLGKNGHQI
                     DPLLEATAGASAVAKGDFNNDGLMDLVSVSSENQPVQLHLLNSQGAFDLLSIAGGGPL
                     AIMNDVDVADFNRDGKLDIAVLVKDTGFVPPKDVDMPSAVILLIQGADPLNPSNWIWV
                     APPLGCHVNPQNPECGMFLSSDQTGPTDLEIGDFNNDGLPDFVIAANGPKPKGADAQA
                     PHTFVLVYLNPGINSAANPAAWNPLVADSDVLTYSDLAVADMDNDGDMDIVAAVPEAK
                     TWNLHWLRNENNGTTWTSVLIGEQTGGADYIDVGDINEDGSPDVVVAGSAYSLIQWFR
                     SPGPANLAPSAPQVPWYVYNIGLISDIEVDGQSQTIEFNQLRVLDLDSDGEVEVFVTG
                     DGYAYEFQRTANLFNPWTGTALFKADPEGVLGRCAFHDYTGNGWLDILVPVDRDGLTL
                     DGVYLFAR"
     gene            complement(2439..5396)
                     /gene="pgmB"
                     /locus_tag="P5316_15845"
     CDS             complement(2439..5396)
                     /gene="pgmB"
                     /locus_tag="P5316_15845"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HRR86482.1"
                     /translation="MSIRHIIRGVLFDLDGVVVFTDKYHYLAWKRLADEQGWQFDEEL
                     NHQLRGIPRMPALQIILDHNHVVIDDAEKESLAARKNGYYKQMIESINDGDLYPGVVP
                     FIEKLRARGIKTGLCSSSKNAGAVLEKLGLRRLFDAVVTGYDFSRAKPDPEIFLLGAE
                     RLGVPPFHCLVFEDAFSGIEAALAARMKAVGVGSKDILTNAPEVISRYDEIDIDALLD
                     AGRPSRLPVEPWTIAETRINRNRAQYWESLLALTNGFMGLRGTYEEDDPVLAEFAYPG
                     MFLNGIYDYEPYHHVVSFPGYPQRRHVMLNICDWRVINLIVDGERFSMFSGKLSDYRR
                     ELDMRRGIVTRGFVWESPSGKRVRVRTTRLVSMARRHSAVIRYEVASLNFEGPIVLES
                     IVRGKARSGELKNDCIEIIEQGSAGEARYFVSRPKTADSRVAMAFVHRLEGAGRSSQS
                     AMEECNGQFIERFTVQAAKNSIITLDKFACFHTSIETPLWDLLPTAVSGVIRDVNDGF
                     ETLLREQTDFWSRYWEVADVQIDGNAADQQAVRFAIYHLRQSNPEDDHRSISACGMTG
                     DHYWGHVFWDTEMYMLPHFIYTQPETVRPLLMYRYHLLDQARRRAREMGGVGALYAWN
                     SISGEECGVVYEASTAEYHLVSDIAHAIWRYVSATGDTEFLCHFGAEIVFETARFLAD
                     RGKFIPLRDNKFCINVVCGPDEYGCGINNNCYTNMLAQWHFRYACRVYDEMERCRPAE
                     FERLVARIGLTPGERRLWQRAADKMYIPYSEELGIHAQDDSFLYLDPVDMSKLPLFTD
                     LRNHTHPLNLWRMQVAKQADVVLLMFVLGDRFAIEQKRANYDYYEPRTNHGSSLSSSI
                     HSIVASEIGRSEDAYNYFRHSALMDINDFKDNTSGGVHSACLGGTWMAVVNGFAGMRD
                     YEGGLQFNPRLPAAWESFRFKVRYRGRLIGVDVGRDGATYRLLEGEDVEFAVGGQTIR
                     LSPQNPLALSQVR"
     gene            6209..9439
                     /locus_tag="P5316_15850"
     CDS             6209..9439
                     /locus_tag="P5316_15850"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRR86483.1"
                     /translation="MSAKLGIDVGAVSVKAAILLPSDQAESAMAALGDGVGGFRRLQV
                     PAARTVLVAPYRRTRGRPLEAVRQVLRELLDKLGADRIEAVALTGSGSGLVAAALGVP
                     RFNEFQAIARAVDLLHPHVRTVFEMGGETSKYIRLVPDPADGRLGIADYGMNGDCAAG
                     TGAFLDQQASRLQYEVEDIGTVVQDARRTAQIAGRCSVFAKSDMIHAQQKGFAPPEVL
                     KGLCKAVAMNYKSAVVKGRTPERPVALIGGVSANTAVVHELAEVFGLKDGDLFVPPAA
                     ESIGAIGAAILAGEASIADRVALGGRLSEVITAEPAGQDGLPRLPRLTLDKVQLLRER
                     VRPYEFPENGQVIDAYLGLDIGSVGTKLVLIDAQGEVIHHIFTRTEGRPIEVVTRCLR
                     ELRDAVGDRVRVCGVGSTGSGRELIGELVGADAIHDEITCHKTGAAFIGDRLIGKRPD
                     TIFEIGGQDSKFISLQPEAGDSAESVVVDFTMNEACAAGTGSFLEERAEELDVSIKGE
                     FGQLALQSKSPIKLGERCTVFMERDVNTCMHRGAKREDIIAGLAYSVVYNYINRVVRG
                     RKIGDCIFFQGGTAYNDAVAAAFSAVTGKEIIVPPHNAVLGAIGAALLAKEKTQAAGN
                     GTRFRGFDMNRVTYTLREFTCKGCGNHCAVQEFNVEGEKTYWGDKCSDRYRKRAKTDR
                     KPVIPDLLAVRHDLLHADDNGDPPHATLTIGVPLAMYTFDLLPLWRRFFRDCGFKVVT
                     SEPTNKITVKAGTDAIVAEPCFPIIVAHGHVADLVAGKVDFIWLPNIISAETKFLDNE
                     SHVCPWGQTLPFVVRQSPAFAAWQDRILCPTIRLRSDTTNLREVLLDVAVRLGARRRV
                     AQRAYEAGINALRQFRDRYLEAGRTALETLTRSGETGMVLVGRPYNIHDPGVSLAVAR
                     KLRDNYGVNVIPIDALPVSDVDVRDINDNMYWEYGRKILAAARIVSRHPNLHIIYVTN
                     FKCGPDSFVKGFVRSASGGKPFLTLQFDGHSNDAGMMTRCEAYLDSKGILRWWRKQQH
                     EPTERASKDGPSISHKWPTPVPDSWPLVSVPSGSKRSPLRTPIPRP"
     gene            9553..10575
                     /locus_tag="P5316_15855"
     CDS             9553..10575
                     /locus_tag="P5316_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86484.1"
                     /translation="MMPTAHGPCRFGQYGPYLRKQLEELGYGETMVFSPTSANGYSDI
                     GQGAGQFIRNAWMGVVCGDIAQKLLFKTRPYELRAGDSDEAFRDAVDRFGRVLSKPDL
                     KPKHRLVELTQVVTQVRDRFRSIPARYEKGRPLIGIVGEIFCRHNTFSNDDLARRVEK
                     LGGECWISDIAEWIWYVDWYVRDRIVRSNGRLNLELFKQWAKSKVQQHYEHILLAPLQ
                     EDFRGLEEPHDIREVLQAGERYLPPQGCIGEMVLSTGKTVYLHRKGADGVIDISPFTC
                     MNGIICEAVYPAVSRDCDGMPIRVFYFDGTQTNLDRDLEIFLDLARAYQRRKKYARVY
                     SHRFDD"
BASE COUNT         2136 a         3546 c         3269 g         2012 t
ORIGIN      
        1 tgcgtctcga gccgcaaaca cccctttctc accccgcagc ctcttcgagc cgatatcgct
       61 attgagcggg catcaccggg gtgcccagcc cgcgtgtcgc ccccggcaga gaagccacgc
      121 ctggaggcat tgttcaaggg gatacactgg gaaaggacgg acatgcgact cggactggaa
      181 aagaaaaagc attctctgtt gcggacgctc gcatgcctcg cgaccggcgg aatggtgttg
      241 tcggtcatcg gctgcgaggg agaggtcggc ggcgttatgg tcgacgccct ggaggccgct
      301 ctgcaggcgg ctatcccagg tttgatggat ctgctcagag ctgacgtggt caacgacggc
      361 agcggcacgg gcaccggtgg cggttcgttg cccacggtaa tgcacgaagc ggtccagatg
      421 gtccggctga tgctggcata acctgcgaat ccgcaggtca ggcaggtcgg ccgtcgactc
      481 ctgcccgcaa cagcggaacg ggccgaaagc tccgcattct cacgatacct caggccggtg
      541 gttccggttg attggggaca ggcctgtgca tgcacaatgc acaggccttc ttcctttggc
      601 ccgcccgccg gcaggcgcgg ttgtactcaa cgatgaccgc ggattctgcg gacctcaact
      661 gcacgctgtt ttcggttcgt gattcgcccc cgaatagggc tcggtgccgg attggaaaat
      721 cgggtaccag atctgcccgc agaaccctga acctacgagc gccaggattc ggttgaggcc
      781 ggcaagtgac ccgacagtgt cggccacggc gccatacttc agccctgccg catgctccgc
      841 ccgccgcagg cgcttcagcg agcaaacaga tagacgccgt ccagggttaa tccgtcgcgg
      901 tcgaccggca caaggatgtc caaccagccg tttcccgtgt agtcatgaaa cgcacaccgg
      961 cccaacacgc cctcaggatc ggccttgaag agcgcagtgc ctgtccacgg attgaagagg
     1021 ttggcggtcc gctggaactc ataggcatag ccgtcgccgg ttacgaagac ctcaacctct
     1081 ccgtcgctgt ccaggtcgag tacgcggagc tgattgaatt cgatggtctg cgactgcccg
     1141 tcgacctcga tgtcgctgat cagaccgatg ttgtacacgt accacggcac ctgcggcgcg
     1201 ctcggagcga gattggccgg tccgggactg cggaaccatt ggatcagcga gtaggcgctg
     1261 ccggccacca ccacatcggg cgagccgtct tcgttgatgt cgccgacgtc aatgtagtct
     1321 gcgccgcctg tctgctcgcc gatcagcacc gatgtccagg tggttccgtt gttctcattt
     1381 cggagccagt ggaggttcca tgtcttcgct tccggcaccg ccgccacgat atccatatct
     1441 ccgtcgttgt ccatgtcggc gaccgccagg tcggagtaag tcagtacgtc gctgtcggcc
     1501 accaggggat tccatgcggc cggattggcc gcactgttga ttccaggatt gagatacacg
     1561 agcacaaagg tgtggggagc ctgcgcatcg gcgcccttcg gtttgggccc gttcgccgca
     1621 atgacgaagt ccggcaaacc gtcgttattg aagtctccga tttccaggtc cgtcgggccc
     1681 gtctggtcgg agctcaaaaa catcccgcat tccgggttct gcgggttaac gtggcagccc
     1741 aagggtggcg cgacccagat ccaattggat ggattgagcg ggtcagcgcc ctgaatgagt
     1801 agaatgactg cggagggcat atctacgtcc ttgggtggta caaaacccgt gtccttgacc
     1861 aggacggcaa tatcaagctt cccgtcccgg ttgaagtccg ccacgtcaac gtcattcatg
     1921 atcgccagcg ggccgccgcc ggcgatcgac aacaggtcga aggctccctg gctgttcaac
     1981 aaatgaagct gaacaggctg gttctcggac gacaccgaga ccaggtccat gaggccgtca
     2041 ttgttgaagt cgcccttggc caccgccgag gctccggcgg tcgcttccag cagcgggtca
     2101 atctggtggc cgttcttgcc gagattgaag agcccgtcca gaatgccggt tcccccggaa
     2161 tcgggcgagc agcccggcgt cagcccgacc agcggcacca gaatgataag atgcgtgcgc
     2221 aaagccttca tcgagtggtt cctttcttct ttcccgggcg acgatcatgc cggaccgccg
     2281 ggacggcagc cgctgcgcgt ggaggtgccc ggagtccctt tgactccgtc ggcatcacgg
     2341 gctcgctgct tcactaacac gagtgtcccg gcggatatcg ggccgctggg tgccttgccg
     2401 gcccggtcgt tcacgttatt gggcctgggg tccgtcccct aacgaacctg cgatagggcg
     2461 agaggattct gcggcgataa ccggatggtc tgacctccga cggcgaactc aacgtcttct
     2521 ccttccagga ggcgataggt ggctccgtca cggccaacgt ccaccccgat cagccgacca
     2581 cggtagcgca ccttgaaccg gaaggactcc cacgccgcgg gcagtcgcgg attgaactgc
     2641 aagccgcctt catagtcccg catgcccgca aacccgttga ccaccgccat ccacgttccg
     2701 cccaggcacg ccgagtggac gccgccggaa gtgttgtcct tgaagtcgtt gatgtccatc
     2761 aatgcactgt ggcgaaagta gttatacgcg tcctccgatc gcccgatctc cgaggccacg
     2821 atgctgtgaa tcgaggacga aagcgacgag ccatggttcg tccgaggctc gtaataatcg
     2881 tagttggccc tcttctgctc gatcgcgaac cgatcgccga gaacaaacat cagcagaacc
     2941 acgtcggcct gcttggcaac ctgcatccgc cacaggttca gcgggtgcgt gtggttccgc
     3001 aggtcggtga acagcggcag cttgctcatg tccaccggat ccaggtacag gaagctgtcg
     3061 tcctgcgcat ggatgccgag ttcctcagag tacgggatgt acatcttatc ggcggcccgt
     3121 tgccagagtc ggcgctcccc gggcgtcagg ccgatccggg cgaccaatcg ctcaaactcg
     3181 gcgggcctgc agcgctccat ctcgtcataa acccggcagg cgtaacgaaa atgccattga
     3241 gcgagcatgt tcgtatagca gttgttgttg atcccgcagc cgtactcgtc cggaccgcac
     3301 accacgttga tgcagaactt gttgtcgcgc agcgggatga acttgccccg gtcggctaga
     3361 aagcgggcag tctcgaaaac gatctccgcg ccgaagtgac agaggaattc cgtgtcgccg
     3421 gtggccgaga cgtaccgcca gatcgcgtgg gcgatgtcgc tgaccaggtg atactcggcg
     3481 gtggacgcct catagacgac gccgcattcc tcgcccgaga tcgagttcca ggcataaagc
     3541 gccccgaccc cccccatctc ccgcgcacgc cggcgggctt gatccaacag atggtaacgg
     3601 tacatgagca gcggccgaac cgtctcgggc tgcgtgtata tgaaatgggg caacatgtac
     3661 atctcggtat cccagaacac gtgaccccag taatggtcgc cggtcatgcc gcaggcgctg
     3721 atgctgcgat gatcgtcttc cgggttgctc tgccgcaggt gatagatggc aaaccgcacc
     3781 gcttgctgat cggccgcgtt gccgtcaatc tggacgtccg ccacctccca gtaccgcgac
     3841 cagaaatccg tttgctcacg caacagcgtc tcaaagccgt cgttgacgtc gcgaatcacg
     3901 cccgaaaccg ccgtcggaag caggtcccac agcggtgtct cgatgctggt gtgaaaacag
     3961 gcgaacttgt ccagtgtgat gatcgaattc ttcgccgcct ggacggtgaa ccgctcgatg
     4021 aattgcccgt tgcactcctc catcgccgat tggctcgaac ggccggcgcc ctccaggcgg
     4081 tgaacgaacg ccatggccac gcgcgaatcg gccgtcttgg gccggctcac gaagtaacgc
     4141 gcctcgccgg cgctgccttg ctcgataatc tcgatgcaat cattcttcag ctcgccgctg
     4201 cgagccttgc cacggacaat gctctcaaga acgatcggcc cctcgaagtt cagcgacgcg
     4261 acctcatatc gaatcaccgc gctgtgccgt cgagccatgc ttaccagcct ggtcgtgcgt
     4321 acgcgaacac gcttgcccga cgggctttcc cagacaaagc cgcgcgtcac aatgccccgc
     4381 cgcatgtcca gttcccggcg gtaatcgctg agcttgccgc tgaacatgct gaaacgctcg
     4441 ccgtcgacga tgaggttgat gacccgccaa tcgcagatgt tcagcatcac gtgccgacgc
     4501 tgcggatagc cgggaaagct gaccacgtga tgatacggct cgtaatcata aatgccgttg
     4561 aggaacatgc ccgggtaggc gaactcggcc aggaccggat catcctcctc gtatgtgccg
     4621 cgcaaaccca tgaacccgtt ggtcagcgcc agcaggctct cccaatactg cgcccggttc
     4681 cggttgatcc gcgtctcagc aatggtccaa ggctcgaccg gcagccgcga gggacggccg
     4741 gcatccagca aggcgtcgat atcgatttcg tcatagcggc tgatcacctc aggcgcgttg
     4801 gtcagaatgt ccttcgagcc gacccccacg gctttcatcc gggcggccaa ggcagcctcg
     4861 atcccggaaa aggcgtcctc aaacaccagg cagtgaaacg gcggaacccc cagacgctcg
     4921 gcgcccagta ggaaaatctc cggatcgggc ttcgccctgc tgaagtcgta acccgtcacc
     4981 acggcgtcga acaagcgcct cagacccagc ttctccagaa ccgctccagc gttcttgctc
     5041 gacgaacaca gccccgtctt gatacctcgc gcacgaagct tctcgatgaa cggcaccacg
     5101 cccgggtaca gatcgccgtc gttgatcgat tcgatcatct gcttgtagta gccgttctta
     5161 cgggcggcca gcgattcctt ttccgcatcg tcgatcacca cgtggttgtg atcaaggatg
     5221 atctgcaggg cgggcatccg cggaatgccg cgaagctggt ggttcagctc ctcatcaaac
     5281 tgccaaccct gctcatcggc caggcgcttc caggccaagt agtgatactt gtccgtaaaa
     5341 accacgacac cgtcgaggtc gaaaagcacc ccacgaatga tatgccttat gctcacccgg
     5401 tcatactccc atggtccgag gtctccgctt tggggccaag aatctatccg gaaccgccca
     5461 aacgtgccac caagcccgcc tgatcgtgcc atccggagac ggccggtcca acgtgccgtt
     5521 cccccgcccc cttcgcgcgc atcccctgcg cctctccccc tcgccgaggg ggagccggag
     5581 ggggtcattc tctccccctc accgaggcag tcattctctt tgggtctcct accttctgcc
     5641 caaccgctct ggcccgcgga aaaccgaagg ggctcgcaga cagggatcac gaggcagcaa
     5701 cgtggcaagg gagcgagaca gaagggcagt gtggccgcaa ggcggcgagg cagcaaggaa
     5761 gcagagcaag caggcaccgc gggttccaaa atagggacga cgccctcgtc agccttgttt
     5821 cgtcgccatg gccccaacac gcaagatccc ggcaagacaa cgccccggcc aggcacctgc
     5881 tctgaacaag ggacggcgtc gcccccgcag cacaaccggg gctgcttcat aaatcagctc
     5941 gaggcgacag gcacacccat cagtcccaaa tcgttctctc ccgtgaacgt aaaacacgct
     6001 gaggtcaagg ttttggggtt ccgtgtttca tacgagcggt atctgcggac ttggccccca
     6061 gacgcaatgc cgtctcgcgc cgccctgtgc ccactcacgt gtggccggca agggcttgac
     6121 aaccgctttg ttagtgttta ctattttcgc ttgtctgaac gtgccaagtg tcgtcgtgtg
     6181 gcgcggcggt gagcaaggct gggactgcat gagcgcgaaa ttgggcatcg atgtcggcgc
     6241 ggtgagcgta aaggccgcga ttctcttacc gagcgatcag gcggaatccg ccatggccgc
     6301 cctcggcgac ggagtcggag gctttcgccg gttacaggtg cccgccgccc gcacggtact
     6361 ggtcgccccg tatcggcgca cccgcggacg gcctttggag gctgtgcggc aggtactgcg
     6421 agagctgctc gacaaactcg gtgccgaccg catcgaagcg gtagccctga ccggttcggg
     6481 aagcggcttg gtcgccgccg cgctcggggt cccgcgtttc aacgaattcc aggccatcgc
     6541 ccgggccgtc gatctgctcc acccgcacgt tcgcaccgtc ttcgagatgg gcggagaaac
     6601 cagcaaatac atccgactcg tgccggatcc ggccgacggc cgattgggca tcgctgacta
     6661 tggcatgaat ggcgactgtg ccgcgggaac cggtgccttc ctggatcagc aggcgtcgcg
     6721 gcttcaatac gaggtcgaag atatcggcac ggtggtccag gatgcccgac gaaccgccca
     6781 gatcgccggc cggtgcagcg tttttgccaa gagcgacatg atccacgccc agcagaaggg
     6841 cttcgctccg cccgaggtac tcaagggcct ctgcaaggct gtggccatga actacaagtc
     6901 cgcagtcgtc aaaggcagga cgcctgaacg ccccgtggcc ctcatcggcg gcgtctcagc
     6961 aaacaccgct gtggtccacg aattggccga ggtgttcggc ctgaaagacg gcgacctgtt
     7021 tgtgccaccg gccgccgagt ccatcggcgc catcggtgcg gccatccttg ccggcgaagc
     7081 ctcgatcgcg gaccgcgtgg ccctcggcgg acgactctcc gaagtcatca ccgccgagcc
     7141 cgcggggcag gatggccttc ccagactgcc gcggctgacg cttgataagg tgcaactcct
     7201 gcgggaacgc gtcaggccgt atgagttccc ggaaaacggc caagtcatcg acgcgtattt
     7261 aggcctcgat atcggctccg tgggaacgaa actcgtcctg atcgatgccc agggcgaagt
     7321 gatccaccac attttcactc ggaccgaagg gcgaccaatc gaggtcgtaa cccggtgcct
     7381 tcgcgagttg cgcgacgccg tcggcgaccg cgtccgtgtt tgcggcgtcg gttccaccgg
     7441 tagcggccgc gagttgatcg gcgagctcgt gggcgcggac gccattcatg acgagatcac
     7501 ttgccacaag accggcgctg ccttcatcgg agaccggctc atcggcaaac ggcctgatac
     7561 gatcttcgag atcggcggac aggacagcaa gttcatcagc cttcagcccg aggccggtga
     7621 ctcggccgag tcggtggtcg tcgacttcac catgaacgaa gcctgcgccg ccggcacggg
     7681 cagcttcctc gaagagcggg ccgaggaact cgacgtctcg atcaaaggag aattcggcca
     7741 actggccctg caatccaagt cgccaatcaa gctgggtgag cggtgcaccg tcttcatgga
     7801 gcgggacgtg aatacctgca tgcaccgcgg agccaaacgc gaagatatca tcgccggcct
     7861 ggcctattcg gtggtataca actacatcaa ccgagtggtc cgcggacgaa aaatcggcga
     7921 ttgcatcttc ttccagggcg gaacggccta caacgatgcc gtcgcggcgg ccttcagcgc
     7981 cgtgaccggc aaggagatca tcgtaccccc gcacaacgcg gtcttgggtg cgatcggggc
     8041 ggccttgctg gccaaggaga aaactcaggc cgccggcaac ggcactcgat tccgcggctt
     8101 tgatatgaac cgcgtcactt acacgttgcg agaattcacc tgcaagggtt gtggcaacca
     8161 ctgcgccgtg caggaattca acgtcgaggg cgaaaagacc tactggggcg acaagtgctc
     8221 cgaccgctat cgcaagcggg ccaagaccga tcgcaagccc gtcattcccg acctcctcgc
     8281 cgtgcggcac gatctgctcc atgcggacga taacggcgat cctccccacg caaccctgac
     8341 catcggcgtt ccgctggcga tgtacacctt cgatctgctg ccgttgtggc ggcgcttctt
     8401 ccgtgattgc ggcttcaagg tcgttacgtc cgagccgacg aacaagatca ccgtgaaggc
     8461 gggcaccgat gcgattgtcg cagaaccttg cttcccgatc atcgtcgccc acggccacgt
     8521 ggccgacctg gtcgccggaa aggtggattt catctggctg ccgaacatca tctcggccga
     8581 aacgaagttc ctggataacg aaagccacgt ctgcccctgg ggccagaccc tccccttcgt
     8641 cgtgcgccag tcgccggcgt ttgcggcgtg gcaggatcgg atcctgtgcc cgacgattcg
     8701 attgcgctcg gacacgacca atctgcgcga ggtactgctt gacgtcgctg tgcgcctggg
     8761 agcccggcgt cgcgtggctc agcgggctta tgaggcgggc atcaatgcct tgcgacagtt
     8821 tcgcgaccgc tacctggagg ccggaaggac ggccctcgaa acactcacgc gatccggcga
     8881 gaccggcatg gtgctcgtcg gccggcctta caacatccac gatccgggcg tcagcctggc
     8941 ggttgcccgc aagctccggg acaattatgg cgtcaacgtc atcccgattg atgccctgcc
     9001 ggtgtccgac gtcgatgttc gcgacatcaa cgacaacatg tactgggaat acggtcgcaa
     9061 gatcctcgcc gcggcaagga tcgtcagccg gcacccgaac ctccacatca tctacgtgac
     9121 caacttcaaa tgcgggccgg attccttcgt caaagggttt gtccggtcgg cgtcgggcgg
     9181 aaagccgttc ctgacgctgc agttcgacgg acactccaac gacgccggca tgatgacccg
     9241 ctgcgaggcg tatctggaca gcaaagggat tctgcgatgg tggcgaaagc agcagcacga
     9301 gccgacggaa agagcctcca aggacggacc ctctatctcc cacaaatggc ctacgccggt
     9361 gcccgactcc tggccgcttg tttccgttcc atcgggatcg aagcggtccc cactccggac
     9421 tccgatcccc agaccctaga gcttggcggt ctctacagca gcggcgagga gtgtctgccc
     9481 cacagaatca ctctcgggga cttcctccga atctgccgtc gtccggactt cgagcccgcc
     9541 aaaacggcgt tcatgatgcc gactgcccac ggcccctgcc gttttgggca gtacggcccc
     9601 tatctgcgca agcagctcga agaactcgga tacggcgaaa cgatggtctt ctccccgacc
     9661 agcgccaatg gctacagcga tatcggccaa ggcgccgggc aattcattcg caacgcctgg
     9721 atgggcgtcg tgtgcggcga catcgcccag aagttgctgt tcaagacacg accttatgaa
     9781 ctccgtgccg gcgacagcga cgaagccttc cgcgacgcgg ttgatcggtt cggccgggtc
     9841 ctgtcaaagc cggacctcaa gcccaaacat cgtctggttg agttgacgca ggtcgtcacg
     9901 caggtgcgcg accgttttcg ttcgatcccc gcgcgatacg aaaagggcag gccgctgatc
     9961 ggcatcgtcg gagagatctt ctgccgccac aataccttca gcaacgacga cctggcgcgc
    10021 cgcgtcgaga aactgggcgg cgagtgctgg atctccgata tcgccgagtg gatctggtac
    10081 gtcgactggt acgtcagaga cagaatcgtc cgttccaacg gacgtctgaa tctggagctg
    10141 ttcaaacagt gggccaaatc gaaggtccag cagcactacg agcacatcct gctcgccccg
    10201 ctacaggagg attttagagg tttggaagag ccgcatgata tccgcgaggt cttgcaggcc
    10261 ggcgaacgct acttgccccc ccaaggttgc atcggcgaga tggtgctaag cacgggaaaa
    10321 accgtctacc tgcaccgcaa gggtgccgat ggtgtcatcg acatcagccc gttcacctgc
    10381 atgaacggca tcatctgcga ggcggtctat ccggcggtga gccgcgactg cgacggcatg
    10441 cccatccgcg tcttctactt cgacggaacc cagaccaacc tcgaccgtga cctcgaaatc
    10501 ttcctcgact tggcccgcgc ctaccagcgc cgcaagaaat acgcccgcgt ttactcgcac
    10561 cggtttgacg actgagcggc tggcccctgc gactcttgtg aaccgcaaca acactctgtg
    10621 gaggacgaac gtcttgccgc tcgcgtcgac ccgccgcaag ccggacccat gtccctgggc
    10681 ccaaacccga aatcgcccga tgccccggag gggcacgggt atgtagccag ggacgcaagc
    10741 ccctgggacg tctgctccgc tccgatccgc ctgtccggaa caccccgcgt gttcatctcg
    10801 cagtgggcga actccgccca ccgaaggaat ggctgacagc taatatctga caacttgtga
    10861 aacgaaggag ccgccaatgt ccacaaagaa atccgccaac ccaacctccc tgtcggcgcc
    10921 ggtctgaaca tgctgtaaat cggccggttt gatgatgcgg tga
//
LOCUS       DAOTPW010000192        10918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5648_length_10918_cov_4.713063, whole genome shotgun sequence.
ACCESSION   DAOTPW010000192 DAOTPW010000000
VERSION     DAOTPW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_10918_cov_4.713063"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="P5316_15860"
     CDS             <1..1053
                     /locus_tag="P5316_15860"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86485.1"
                     /translation="GLGFGSGGENTGFVYDATTGVIGHIVAGGYQACVDGIGYWTDGT
                     TTKVVIEGDNGGWQSMNAWDTASNWDGTAQWAKIRRTNTHAYGDNGGLNRMAGTSNSS
                     TGLLHGVFQTNTQTIYIDAFNGTGLGALDVKSTTNETTMNGISNAGKAVGVRGGRCYY
                     VGPYVGGTTPANGYIAAAPDGTDAGSLYDIADDGSIMGGYGHVTGKTGWYPFVYDGTT
                     SFELPGLSSGSGYATNGVVYSISPNGDYAVGMDYTKGYELAVMWDLRDMNNIVATDLT
                     AFAAANGILGPFDGNLRRATAIGINAEGNPVIAGVGVASSLEASGYTGFVLTIPEPAT
                     MGLLVLGLPLMFWRRR"
     gene            1397..3325
                     /locus_tag="P5316_15865"
     CDS             1397..3325
                     /locus_tag="P5316_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86486.1"
                     /translation="MSLSRVGFAGAVILMMTAGALAAPVFTEVPPWTGPSNAGATFTG
                     ERTDIRAMTPDGTYLVGNCTGLDNGSTPATYGVIWDATNGSRKVLSSDGALATTCTGA
                     DYRIVDGSTQLVVAGLSAGWATAWFSNDGGISWLKKARATNVGSSPSLANGNTLRGTS
                     TDVAYFIWKDGDKYHYTSMVVGTTNPPSLTNTPKSISSGKMLMNSVANTGRVAGRRYD
                     SAAPYLITLFEYPPAGGNQTQLPGLDGTLRGEVWAVNADGTQLFGISPVSDGRAGNWP
                     FKMVISGVHPNVTQVSINELPTYPDTAGSTSNGLPYGASADGTFACGMNYRGQERAVL
                     WDLRDAEPANWKVYDLTSYLAASGNLGSFSRLNRAHTVAVNANDVVVAGIGVLSTTGK
                     TTGWCLRIPLADLPFPDTGACCMIGACGDKFAADCPDIEGQQRWTANKLCANAACPGA
                     CCTDSMTGACSDFVESATCTDGGGVFRGASSACSAGVCLASCCKTDGTCGAEPPGYCE
                     SIDGIAGALGSVCEASTCLGACCVKAGQCEEMNYGVCPAAKFKGIGTSCATEECPCSI
                     PFADADGDGDVDQADFALFQVCIGANLPAEPACACFDAHPAGAPDGDVDTDDWSAFEA
                     CASGSGITADPACGGVLP"
     gene            complement(3543..4724)
                     /locus_tag="P5316_15870"
     CDS             complement(3543..4724)
                     /locus_tag="P5316_15870"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRR86487.1"
                     /translation="MKISQRVQELEESATLAVSAKAAAMKKQGINVVGFGAGEPDFPT
                     PVNVRNACKAALDAGHTKYASPSSGIPAAKEAVCSKLKRENNLEYKPSQVMITVGGKE
                     ALYLASMSLLDPGDEAILPAPYWVSYPEQIKLCGAKPVIIEGKESNNFKVTPDQIAKA
                     ITAKTRVLIFNSPSNPGGFTYAPDEVRAIAKLLADKDIIVFSDEMYDRLIYGNQRFLS
                     FAACSPENYAKTLTFNAGSKTYSMTGWRVGYVAGPEPIIKAMAKLQSQTTSGTATFCQ
                     HALVEALSGDQSEVEKMRKEFERRAEYMHQRLNGIKGVTCVQPTGAFYAFPNVSGAFA
                     ALGVKGSLDFSAKVLERAHVAVVPGIAFGLDDYVRLSFATSMEQIREGCDRLEKLLGK
                     K"
     gene            complement(4936..5937)
                     /locus_tag="P5316_15875"
     CDS             complement(4936..5937)
                     /locus_tag="P5316_15875"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRR86488.1"
                     /translation="MSQRAGRHSPLLRWVIPLSNAYRRMLAIVLGLVGPRVAYRVTGR
                     LARILYRSLQPVRARSEAQCRAALGERVPSEDIPRIAEASFVHRIWNLTDLMLADRLL
                     HRNTCHHYGGRIPEPYLNMLLNARRDKQPVILVTAYYGPFDLLPLFLGCNGVRATVVY
                     RPHRNAGFDAYRRRIRERGGCELVPLDRASNRIAQRLEEGRTVAILADHHAERRGLPA
                     TFLGLPTMALRSVGWLAHHYRAAVAVAGIRRVGQKFRFEIVVSGVIQPSDWASAEDPV
                     AYITTQYLAALERIILSEPIQYLWSYARWGEATARRLAESPDPAGSGGSTTPVTAKR"
     gene            complement(5976..7289)
                     /gene="xylA"
                     /locus_tag="P5316_15880"
     CDS             complement(5976..7289)
                     /gene="xylA"
                     /locus_tag="P5316_15880"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HRR86489.1"
                     /translation="MREFFPKIKKIVYKGPKSKDPLSFKHYNPDEKVFGKTMAEHFRF
                     SVCYWHTFKGTGADTFGGPTFIREYNKSSDPMQVAEMTLQAAFEFYTKLGVGFWCFHD
                     RDIAPEGRNLSQTNANLDRIVAMARKLQRDTGVKCLWGTANLFSNRRYLAGAATNPSP
                     HVFAYAAAQVKKAMEVTKELGGAGYVFWGGREGYETLLNTDMKRELDHLAQFLHMAVD
                     YKKKIGFRGQFYIEPKPKEPTKHQYDSDAGSCYAFLQKYDLVDHFKLNIEANHATLAG
                     HTFHHELEFAAANGILGSIDANRGDLLLGWDTDQFPTDLYDTTLAMYTLLRYGGFKTG
                     GLNFDAHVRRQSIDPEDLFHAHIGGMDAFARGLKIAAGLIRDRALSKFVTARYKGWDG
                     ALGRKIEKRQMDFNKLEKYVLANGEPRVQSGRQELIENILNEYIK"
     gene            complement(7424..8554)
                     /locus_tag="P5316_15885"
     CDS             complement(7424..8554)
                     /locus_tag="P5316_15885"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR86490.1"
                     /translation="MRSSRPETILFTGATGFLGHYILRDLLTRGVRIVAVLRPPLDHA
                     KARLETMLAAIGLPVEQYLSNGSLLLVPGSLPDTLPEPDWGRTDAILNCAASLSLFAN
                     GDGEPFNTNVSGTEALAEWAIRHNVIRIHAVSTAYTCGWLQGAIPERFHDPPPEFQTD
                     YERSKWQAEAVLEEWSSWIGHSLTLYRPSFLVGDSTTGHTTQFAGFYQFARLVGALKE
                     RYASKNNGTRTYIPLRIPGRPDDRQNIVPVDFVSRVIAEVVTRPRFHGRIYHLTNPEP
                     PTNDMMKRCYEDYFGLHGGFFAPPDEVLGKCNQAESLLWDQYHLITPRVVHNPQFIMD
                     NTREVMQAAGISFPTLHPERIFMLFDYAARKGWGRGSNGFHH"
     gene            complement(8650..>10918)
                     /locus_tag="P5316_15890"
     CDS             complement(8650..>10918)
                     /locus_tag="P5316_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86491.1"
                     /translation="IDKMYSRRYVILVLLLLGSATAVAHYKAIWDGWFLDDHWHRRQY
                     MDDRWSLKALLDATTIKPDAFIETWWQTENIQWQYIRPVSILVVKALYRLSNGSVKTL
                     HLLSIVLHLVNAVMVHHLCLRLTKMRFWSIVGALLFVVYSHSLFAVAWLAAQNTVLMT
                     TLTLGALLCYVRASGLNLYAAPAADPKPAQAGLHAAEGRNLNGHAPPMRWSWFAGAMF
                     LWVLALGSRENAIVLPLLVASFDLAFGGRRHLRTRLPGLLLMVAVAVMFMIWRLFLDY
                     EPFPDFYLRRPDGPGYFLWLPVKLLHYVTAAIWLSPMTVGPTGRFNPVTEVPGDCLLM
                     LLIVAIMGTGYYQACRRARGWWIWPLWILLALLPVTPIMATPHSGYMPAVAFAVAMVL
                     GAALKDHLAPIGIGKWCRPVAIWFLIATTTYIPIYRTMWQSVLAAERWTIDEITATAG
                     HDEATDVFLINLPFVNIYAKCHLQEAWAGDSDDRLLRTAPDLRCHVLTYADNVLRMQD
                     ACRLEQIDAYTFSLSCAGRGYFSGALGRFCIEAMRAGRRFQTGEIIRNHRAPFEVEIV
                     QADQEAVRELKFRFHKPLATSRYLFYLTSTEHAAARLRFWGPQGPPKVPALRPIHVDD
                     ASVSRDAEALRAGYAGAAEGLFAAVGSGNPRLAQRAWSALQEVARPVSRALAAPLQDV
                     VAGEVPGPPDLSRVRKWWLSTVDDQTLARLTSERDSARRFLKERDMLFRIREVAGRII
                     RTDLYLTGPPYPGPR"
BASE COUNT         2120 a         3153 c         3549 g         2096 t
ORIGIN      
        1 ggactggggt ttggcagcgg tggtgagaac acaggcttcg tttacgacgc cacaacgggt
       61 gtcatcgggc acatcgtcgc gggcggctat caagcttgcg tggatggcat cggctattgg
      121 accgacggca ccacgaccaa agtcgtgatc gagggcgaca acggcggctg gcagtcgatg
      181 aatgcatggg atactgcgag caattgggac ggaacagctc agtgggcgaa gattcgtcgg
      241 acgaatacac atgcctacgg cgacaatggc ggtctgaaca ggatggcggg cacgagcaac
      301 agcagcaccg gcctgctcca cggcgtgttc cagaccaaca cccagacgat ctacatcgac
      361 gccttcaacg ggaccgggct gggtgccttg gacgtcaaga gcacgactaa tgaaaccacg
      421 atgaatggta tctccaatgc aggcaaggcg gtcggcgtac gcggcggtcg ctgctactat
      481 gtaggacctt atgtcggcgg cactactcca gcgaatggct atatcgccgc cgcgccggac
      541 gggaccgacg ccggcagtct gtacgacatt gcggacgacg gtagcatcat gggcggctac
      601 ggccatgtga ccggcaagac cggctggtat cctttcgttt acgacggcac aacttcgttc
      661 gagctgccgg gccttagcag cggctcaggc tatgcgacca acggcgtggt ctactcgatt
      721 tcgcccaacg gcgactatgc cgtcggcatg gactacacca agggttacga gctggcggtc
      781 atgtgggatc tgcgggatat gaacaacatc gtcgccacgg acctgaccgc gtttgcggcg
      841 gcgaacggca tcctgggccc gttcgacggc aacctccgtc gggccactgc catcgggatt
      901 aatgccgaag gcaatccggt tatcgcggga gtcggcgtgg ccagcagtct tgaggccagc
      961 ggttataccg gcttcgtgct gacgattccg gaacctgcga ccatggggtt gctggttctg
     1021 ggtttgccgt tgatgttttg gcgccggcgc tgaggttttg cggcgagttg tgtgaagtct
     1081 gagcgttgag ggttgtggga aagccctgcg ggacggtggc gaagggctgc cgcccgcagg
     1141 gtgaacccgg ggtgcccgag ggagagaatc gaacttcatt gtcgggccgg cgtgcgacga
     1201 gccccgggga tccggccgga gagcgggttt tttgggcgtg ttgaccacac ctgaacggcc
     1261 ccacgggccg ttcaggcagt gtggaatgtg gggttctata tggtgccgtg accccgcggg
     1321 gtcaccggca gggcaacaca acgaaacagc atgggcaaat gcgggccgga tgaagggccc
     1381 ggagggagga tgatgtatga gtctttcgcg agtcggattc gctggagcgg tgatactgat
     1441 gatgacggcg ggtgcgctcg ctgctcctgt gttcacggag gttccgccgt ggaccggtcc
     1501 cagcaacgcg ggcgcaacct tcacgggtga gcgcacggac atccgcgcaa tgacgcccga
     1561 cggtacgtat ctggtcggca actgcacggg gctggacaac ggctcgacgc cagcaacata
     1621 cggcgtgatt tgggatgcca ccaacggcag ccgcaaggtt ctgagcagcg atggggcact
     1681 ggcgacgacc tgcaccggtg ccgattaccg cattgtcgac ggttccacgc aactggtggt
     1741 tgcgggcctg tccgcaggct gggccacagc gtggttctcg aacgacggcg gcatcagctg
     1801 gctcaagaaa gcaagagcaa cgaacgtcgg cagctccccc agtctcgcga acggcaatac
     1861 gttgcgcggc accagcacgg atgtggccta tttcatctgg aaagacggcg acaagtatca
     1921 ttacacgtcg atggtcgtgg ggacaactaa tccaccgtcc ttgacgaata ctcccaagtc
     1981 gatcagcagc ggcaagatgc tgatgaacag cgtggccaac acgggccggg tggccggtcg
     2041 gcgttacgat agtgccgcgc catacctcat tacgttgttt gagtatccgc ccgcaggcgg
     2101 caaccagact cagttgcccg gtttggatgg cactttgcgc ggtgaggtct gggcagtcaa
     2161 cgctgacggc acgcagcttt tcggtatctc gccagtgagc gacgggcgcg cgggcaactg
     2221 gccgttcaag atggtcatca gcggcgttca cccgaacgtg acccaggtgt cgattaacga
     2281 gttgccgacg tatccggaca ccgcaggaag cacaagcaac ggcttgccgt atggtgccag
     2341 tgccgacggg acttttgcct gcggcatgaa ttatcgtggt caggaacgtg cggttctctg
     2401 ggatctgcgc gacgccgagc cagccaactg gaaagtttac gacctgactt cctacctggc
     2461 agccagcgga aacctgggct ccttctcccg gctgaaccga gctcacaccg ttgcggtcaa
     2521 cgcgaacgac gtagttgtcg ccggtattgg cgttctgagt acaaccggta agaccactgg
     2581 gtggtgcctg aggattccgc ttgccgattt gcctttcccg gataccgggg cttgttgcat
     2641 gatcggcgcg tgcggggaca agttcgcggc ggattgcccg gatattgagg ggcagcagcg
     2701 gtggacggcg aacaagctgt gtgccaacgc ggcgtgtccg ggcgcctgct gcacggattc
     2761 gatgacgggt gcctgctccg atttcgtgga gtccgcgacc tgcaccgacg ggggcggagt
     2821 gttccgcggc gccagctcgg cttgctcggc gggagtgtgc ctggcttcct gctgcaagac
     2881 ggacgggacg tgcggggcag agccgccggg gtattgtgaa tcgatcgacg ggatagccgg
     2941 tgcgttgggc agcgtgtgcg aggcatcgac gtgcctgggt gcctgttgcg tcaaggcagg
     3001 tcaatgtgag gagatgaact acggcgtctg cccggcggcc aagttcaagg gtattggtac
     3061 gagctgcgcg acggaggagt gtccttgttc cattccgttt gccgatgcgg acggcgacgg
     3121 tgatgttgac caggccgatt tcgcgctgtt ccaggtgtgc atcggggcga atctgccggc
     3181 ggagcccgcc tgcgcgtgct tcgacgcgca tccggccggt gcgcccgacg gcgacgtcga
     3241 caccgacgac tggtcggcgt tcgaggcctg cgccagcgga tcgggcatta ccgcagatcc
     3301 tgcatgtggc ggcgtgctgc cgtaacgatt gcgggtcggt ttagtttcag acctccttca
     3361 tacgaggttt cgtgacgagg tggcctcggc aacggggcca ccttttttgt atcaactctc
     3421 gaaccgcgca gtctgaatga ccgtgcgcaa tcttgcggca aagctcccgc gaaccaccag
     3481 gaaaggcagg gaacagcggc tttccgccgt cgccgaatcg cgcacggtca tatagtgtcg
     3541 atctactttt tccccagcag cttctccagc cggtcgcagc cttcgcggat ctgctccatg
     3601 ctggtagcga aggacagacg gacgtagtca tcgaggccga aggcgatgcc cggcacgacc
     3661 gccacgtggg ctcgttcgag cactttggca gagaaatcca gcgaaccctt gacacccagg
     3721 gcggcgaacg cccccgacac gtttggaaag gcgtagaagg cgccggtcgg ttggacacac
     3781 gtgacgccct tgatgccatt gaggcgttgg tgcatgtact cggctcggcg ctcgaactcc
     3841 ttgcgcatct tttcgacctc ggactgatca ccgctcaagg cctcgaccag ggcgtgctga
     3901 caaaacgttg cagtaccgct cgtggtctgg ctctggagct tggccatggc cttgatgatc
     3961 ggctctggcc cggccacgta accgacgcgc caaccggtca tcgagtaggt tttggagccg
     4021 gcgttgaagg tcagcgtctt ggcgtagttc tccgggctgc aggcggcaaa gctcaggaag
     4081 cgctggttgc cgtagatcag gcggtcgtac atctcgtcgc tgaagacgat gatgtccttg
     4141 tcggccagga gcttggcgat cgcccggacc tcgtcgggcg cgtaggtgaa gccgccgggg
     4201 ttgctgggcg agttgaaaat cagcacccgt gttttggcgg tgatggcttt ggcaatctgg
     4261 tcgggggtga ccttgaagtt gttggactcc ttgccctcga tgatgaccgg cttggctccg
     4321 cagagcttga tctgctcggg atagctgacc caatatgggg ccggcaggat ggcctcgtcg
     4381 ccggggtcga gcaggctcat gctcgccagg tagagagctt ccttgccgcc gacggtaatc
     4441 atgacctggg acggtttgta ttccaagttg ttttcccgct tgagcttgga gcacaccgcc
     4501 tccttggcgg ccgggatacc ggaggacggg ctggcgtatt tggtgtggcc ggcgtccaag
     4561 gcggccttgc aggcattgcg gacattgaca ggcgtgggga agtcgggctc tccagccccg
     4621 aagccgacga cgttgatccc ctgcttcttc atggcggcag cctttgcgct gacggccaag
     4681 gtcgcggatt cctcaagttc ttggacgcgc tgcgagatct tcatacatgg tgctcctgtt
     4741 gctctgtctt gcggtggctg gggctccgaa cacgcgagcc cgttgttcac cggggtgcag
     4801 attgtaggga tgcgaggcag aatgtacaag ctggcgggag gcggcggcga ggaccgcgcc
     4861 cgtccggcca accgcgtgct ccgggttcgc cggttggaca ctcgatcgct tcggcacccg
     4921 aagataagaa cagcactatc gtttcgctgt gaccggtgtg gttgagccgc cggatccggc
     4981 tggatccggg ctctctgcga gtcttcgggc cgtcgcctcc ccccaacgcg cgtagctcca
     5041 gaggtattgg atgggttctg aaaggataat cctctccaag gccgcgaggt actgcgtggt
     5101 gatgtacgcc accggatcct cggccgaggc ccagtcgctc ggctggatga cgccagagac
     5161 gacgatctca aaccggaact tctgaccaac ccgccggatg cccgcgacgg ccacagcggc
     5221 gcggtaatga tgcgcaagcc atcccaccga tcgcaaagcc atggtaggca gtcccaggaa
     5281 agtcgcgggc aatcctcgcc gctcggcatg gtggtcggca agaatggcga ccgtccggcc
     5341 ttcttccagt cgttgggcaa tgcggttgct cgcccgatcc aacgggacga gttcacagcc
     5401 gccgcgctct cgaatccgcc gtcgataggc gtcgaagccg gcgttgcggt gcggccgata
     5461 gaccaccgtc gcgcgaacgc cgttacagcc aaggaacaag ggcaggagat caaacgggcc
     5521 gtagtaagcg gtgaccagaa tcaccggctg cttgtcccgt cgggcgttca ggagcatatt
     5581 gagataaggc tccggaatgc ggccgccata gtggtgacag gtattgcgat gcaaaagccg
     5641 atcagccagc atgagatcgg tcagattcca gatgcggtgc acgaaggatg cttcggctat
     5701 gcgagggatg tcctcgctcg ggacccgttc tccgagagcg gcccgacact gagcctcgct
     5761 gcgtgctcgc accggctgca gcgaccggta gagtatccgg gccaatcgcc ccgtgacacg
     5821 gtaggcaact ctcgggccga cgagtccaag aacaatggcc agcatccgcc ggtaggcgtt
     5881 gctgagcggg atcacccagc gcagcagcgg ggaatgcctg cctgctcttt gactcatgtg
     5941 cctggcgtgt tgcgtcgttt cagcagccct ggggcctact taatatactc attcaagata
     6001 ttctcgatca gttcctggcg gccggactgg accctgggtt cgccgttggc caggacgtac
     6061 ttttcgagct tgttgaaatc catctgccgt ttctcgatct tgcggcccag ggctccgtcc
     6121 caacccttgt atcgggcggt gacgaacttc gacaaggccc tgtctctgat cagtccggcg
     6181 gcgatcttga ggccgcgggc aaaggcgtcc atgccgccga tgtgtgcgtg gaagagatct
     6241 tccgggtcga tcgactggcg gcggacatgg gcgtcgaagt tgagcccgcc ggtcttgaac
     6301 ccgccgtaac gcagaagcgt gtacatcgcc agggtggtat cgtacaggtc ggtcgggaac
     6361 tggtccgtgt cccagccgag gagcaaatcg ccgcggttgg cgtcgatcga gccgagaatg
     6421 ccgttggccg cggcgaactc aagctcgtgg tggaacgtat ggccggccag cgtggcgtga
     6481 tttgcctcga tgttgagctt gaagtggtcg accaggtcgt acttctggag gaaggcgtaa
     6541 caggatccgg catcggaatc atactggtgc ttggtcggtt ccttgggctt gggctcgatg
     6601 taaaactgtc cgcggaagcc gatcttcttc ttgtagtcga cggccatgtg caggaactga
     6661 gcgaggtggt caagctctcg cttcatgtcg gtattgagta gggtttcgta accctcgcgg
     6721 ccgccccaaa agacgtaccc ggccccgccc agttccttgg tgacctccat ggccttcttg
     6781 acctgggcgg cggcgtacgc gaacacgtgg ggcgagggat tggtggctgc tccggccaag
     6841 tatcgtcggt tggagaacag gttggcggtg ccccacaggc atttgacgcc cgtgtcccgc
     6901 tggagcttcc tggccatcgc gacgatcctg tcgagattgg cgttggtctg gctcaggttg
     6961 cggccctcgg gggcgatatc gcggtcgtgg aagcaccaga atccgacgcc aagcttggtg
     7021 tagaactcaa acgcggcctg caaagtcatt tcggcaacct gcatcggatc gctgctcttg
     7081 ttgtattcgc ggatgaaggt gggtccgcca aacgtatccg cgcccgtgcc tttgaacgtg
     7141 tgccaataac agaccgagaa acggaaatgc tcggccatgg tcttaccgaa cactttttca
     7201 tcggggttgt agtgcttgaa cgacaagggg tctttggact ttggtccctt gtacacgatc
     7261 tttttgatct tggggaagaa ttcacgcatg gatcgcacgc ctcctcttaa agaccgctct
     7321 tggatcgcga gccgccggca agccttcgcg ccgccgtgca gacgaatccg attatagtcc
     7381 cgccggggac ccgtgcaacg agcctgagaa gcacgaaggg tgatcagtgg tgaaaaccgt
     7441 ttgagcctct gccccagccc ttccgggcgg catagtcaaa caacatgaaa atccgctcgg
     7501 ggtggagagt agggaacgag atgccggccg cctgcatcac ttcgcgcgtg ttgtccatga
     7561 tgaattgcgg gttgtgaacg acgcgggggg taatcaggtg gtattgatcc cacagcaatg
     7621 attcggcctg attgcacttg ccgaggactt cgtcgggcgg cgcgaagaac ccgccgtgga
     7681 ggccgaagta gtcctcgtag cagcgcttca tcatgtcgtt ggttggtggc tcggggttgg
     7741 tcagatggta aatacgacca tggaaccggg gacgcgtcac aacctcggcg atcacccgcg
     7801 acacgaagtc gaccggcacg atattctgcc ggtcatcggg gcgtccggga atgcgcaggg
     7861 gtatgtaagt ccgcgtgccg ttgttcttcg atgcgtagcg ttccttcaga gcgcccacga
     7921 ggcgggcgaa ctggtagaaa ccagcaaact gggtcgtatg gcccgtggtc gagtccccca
     7981 ccaggaaact aggccgatac agggtcagcg aatgcccgat ccacgaagac cactcttcaa
     8041 gaacggcctc agcctgccat ttgctgcgtt cgtaatccgt ctggaactcg gggggcgggt
     8101 catgaaaacg ttccgggata gctccctgca accagccgca ggtgtaggcg gtgctcacgg
     8161 cgtggatgcg aatgacgttg tgccgaatcg cccattccgc cagagcttca gttccgctga
     8221 catttgtgtt gaagggctct ccgtcaccgt tggcaaagag gctgagactt gcggcacaat
     8281 tcaggatggc gtcggttcgt ccccaatcgg gctcgggcaa cgtgtcgggc agcgaaccgg
     8341 ggacaaggag cagagatccg ttggataaat actgttcgac aggcagtccg atggccgcca
     8401 gcatggtttc gagtcgagcc ttcgcgtggt ccaacggtgg gcgaaggacg gcaacgattc
     8461 ttacccctct ggtcaggagg tctcgcagga tgtagtggcc aaggaagcct gtcgcccctg
     8521 taaatagtat ggtctcgggt cggctactcc tcattaccat atgcactcgt gctcaccttg
     8581 gacaacccct gcccgcgccg gcagggtagt cgcagtatca tagcgtaaac catttgggac
     8641 gccaagggct tatcggggtc cgggatacgg gggtccggtc aggtacaaat ccgtccgaat
     8701 gatcctgcca gccacctccc gaatccggaa gagcatatcc cgttccttca gaaaccgccg
     8761 ggcagagtct cgctccgacg tgagccgagc caacgtctga tcgtcgacgg tcgaaagcca
     8821 ccatttgcgc acccgcgaca agtccggagg tccgggcacc tcgcccgcaa ctacatcctg
     8881 gagcggtgcg gcaagtgccc tgctcactgg ccgggcaacc tcttgaagcg ccgaccaagc
     8941 tctctgagcg agacgaggat tgccgctacc aacggccgcg aacaagccct cagccgcccc
     9001 cgcgtacccg gcccgcaacg cctcggcgtc ccgtgaaact gacgcgtcgt ccacatggat
     9061 cggccgcaga gcaggcactt tcggtggccc ctgcggtccc cagaatcgca gccgggcagc
     9121 cgcatgttcc gtactggtca agtagaacag atatcggcta gtagccaaag gtttgtgaaa
     9181 tcggaattta agctcccgaa ccgcctcctg gtcagcctgc acaatctcga cctcgaacgg
     9241 ggccctgtga ttgcggatga tttcgccggt ctggaaccgc cggcctgccc tcatggcctc
     9301 gatacagaag cgacccaaag ctccggagaa atagcccctc ccggcacagc tcagtgaaaa
     9361 cgtgtaggcg tcgatctgct ccagacggca tgcgtcctgc atacggagga cgttgtcggc
     9421 atacgtgagc acgtggcaac ggagatcggg ggccgtccgg agcagccggt catcggaatc
     9481 gccggcccaa gcctcctgca aatgacactt ggcgtagatg ttcacgaatg gcagattgat
     9541 caggaagacg tccgtggctt cgtcgtggcc ggcggttgcc gtgatttcgt caattgtcca
     9601 tctttcggcg gccaagacgg actgccacat ggttcgatag atgggtatgt aggtagtcgt
     9661 ggcgatgagg aaccagatgg cgaccggacg gcaccatttg ccgataccga tcggggccag
     9721 gtgatcctta agggccgctc ccaagaccat ggctacagca aacgccacag cgggcatgta
     9781 gcccgaatgc ggcgtggcca tgatcggagt gacaggcagc agtgcaagca ggatccagag
     9841 cggccagatc caccagccgc gtgcgcgccg acaggcttga tagtaaccgg tgcccatgat
     9901 cgcgacgatg aggagcatca gcaggcaatc gccaggcacc tcggtgacgg ggttgaatcg
     9961 acccgtcggc cccacagtca tgggtgaaag ccagatcgca gccgtcacat agtgcagaag
    10021 cttgaccggc agccacagga agtagcctgg accatccggc cttcgcagat agaagtccgg
    10081 aaacggctcg tagtccaaga agagacgcca gatcatgaac atgacggcaa ctgcaaccat
    10141 gagaagcaat ccgggcagtc gcgtgcgcag gtgccgccgc cccccaaagg caagatcgaa
    10201 ggatgcaaca agaagaggaa gaacaatcgc gttttcacga ctgcccagag ccaacaccca
    10261 aaggaacatc gcaccggcaa accaagacca gcgcatgggc ggagcgtggc cgttcaggtt
    10321 tcggccttcc gccgcgtgca atccggcttg agccggtttg ggatccgccg ccggtgcggc
    10381 gtagagattc aaacccgatg ctctgacgta gcacagcagc gcgccgagag tcagcgtcgt
    10441 catcagaacg gtgttctgag ccgccagcca ggccaccgcg aacaggctgt gagaatagac
    10501 gacgaaaagc agcgctccga cgatcgacca gaatcgcatc ttcgtcaagc ggagacagag
    10561 gtggtgaacc atcacggcgt tcaccaagtg cagcacgatg ctcagcagat gcagcgtctt
    10621 gacgctgcca ttgctcagac ggtatagggc cttcacgaca agaatggaca cgggacggat
    10681 gtattgccac tggatgttct cggtctgcca ccatgtctcg atgaatgcat cgggcttgat
    10741 cgtggtggcg tcgagcagcg ctttcaagga ccaccggtcg tccatgtatt ggcgacggtg
    10801 ccagtggtcg tcgagaaacc agccgtccca gattgccttg tagtgggcca ccgcggtggc
    10861 gctaccgagc aagagaagaa ccagaatgac gtaccgacga gaatacatct tatcgatg
//
LOCUS       DAOTPW010000193        10885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5666_length_10885_cov_4.696491, whole genome shotgun sequence.
ACCESSION   DAOTPW010000193 DAOTPW010000000
VERSION     DAOTPW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5666_length_10885_cov_4.696491"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="P5316_15895"
     CDS             complement(<1..1149)
                     /locus_tag="P5316_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86492.1"
                     /translation="MVASVFNRRESSWTARRSHRFARLCGTTTLLASTLGMATLPAGC
                     GQLISEEVFRPAVQTAVARALEDMIDADIYVSPDGRLEWSGRLREPNSDKTDGPLPTI
                     QAARDALRRIRTTEKPKNAAAWLVSVKPGTYELEEPLVFGPEDSGTRDTPVMYACFGN
                     EHATISGGRRITGWKTAEVNGKKVWAAELADVKAGKWYPRQLFVNNERRPRTRLPKEG
                     YFELTGLPQMTDKTPWNEGQTEATFKPGDIRNWRNLGDVEVVALHFWIESRLPLAEVN
                     ETENLAKFTRKSIFRLTDAHSPKQFARYYVENVFEALDTPGQWYVDRSTGMLYYVPKP
                     GETPENTTVIAPRLPQLIRVAGDRKAGKPVHDLHLCQLTFSHADWSLPA"
     gene            complement(1489..2142)
                     /locus_tag="P5316_15900"
     CDS             complement(1489..2142)
                     /locus_tag="P5316_15900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR86493.1"
                     /translation="MNTTGVVFDLDGTLADTLPAIADAINYGLAAVGRPEASRDEVRR
                     WIGEGLPTLCRRALPDADKATFERMMAAVSQHYREHQMDKTVPFPGIAELMDELAKRG
                     VLMGVLSNKPHVYTVPMTQGLFGRWPLVAIEGYKEEDRRKPDPRTLLDIVARMKAKPN
                     EVMLVGDSNTDIATARNAGVVPIGATWGYRDREELIAAGAAHLIDAPAQLLQLIWQG"
     gene            complement(2304..3296)
                     /locus_tag="P5316_15905"
     CDS             complement(2304..3296)
                     /locus_tag="P5316_15905"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRR86494.1"
                     /translation="MLPQTYRYVGTVYHFLIATACISGCVTLRPAIAQESESSLRRNI
                     AWQVALENVGFSPGLIDGNPGRKTKIATWEFQRVKGLPKTGELDKATAEALKVDPDGA
                     LILYTIESADLKEVDPVPHSWVAKSKKSLLGYSDLEEALAEKCHCTRGLLQRLNPGKK
                     LSNLKPGDKYIGPAIASSARLPQATQLEVNLNDKIIRVIDRDKQLAGLFHCSIAASKA
                     KLPSGSAEVEFSATDPEYTFDPKMWPEVTEKITSKLTIPPGPRNPVGRCWIQLTLPGV
                     GMHGSPNPEMIGKTGSHGCFRLTNWDALRLAKMVKAGTPVKFVDTPSEAAQKVQ"
     gene            complement(3326..4381)
                     /locus_tag="P5316_15910"
     CDS             complement(3326..4381)
                     /locus_tag="P5316_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86495.1"
                     /translation="MLLQMLVVLCCCEADPASEVSTRPAGDSAKSAHIRAGDLDVVFL
                     DNSESPGVLSGIASLINVKDAPGFNAFDPDSKGAAAGLNFEHIIAGHENPHNAFTPRH
                     GPYRLYTLPDGRSVMLVRNAADDPWAMASTMKYTVTPPHYIDFEFRCRPADASLFGRH
                     RYAILFWANYMNDVEDVAINFRGIERRDGREEWIRVDAPPGHPDHNHGGTWRHVQAKD
                     LPRDDDLRFRLNSWSYEYPRFSKPFYYGRAAKGMTYILMFDRAYTPADELRFSLFKFK
                     LDKFPRPAWDFQYVIHPVEQDREYGYRARAVWKKFISPEDCLAEYESWARSMAGPSVA
                     GQIQSPDAPATAPARRE"
     gene            4542..5396
                     /locus_tag="P5316_15915"
     CDS             4542..5396
                     /locus_tag="P5316_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRR86496.1"
                     /translation="MRQGQEGEGKGTCDALMGRDQAVSRRELLASLAVAVSARAALAA
                     VGTSRPASRPADTIPWDDPPTDAVLGRVLYDGTPPVLAPVDCSADPNCAALYRHNPLL
                     PEDLVVSKTGGLAHVFVSVKHGLDPGRRWPVPDRPVILDQKGCLYIPHVFGVMAGQPL
                     QILNSSKINEVPHGYPKRNPEFSFTLPKRGMSKTVVLNEPEVFRIGCDVHPWETAWCH
                     VMAHPFFAVTDTEGRFMIRGLPAGEYELELWHEHATLGTLTRSVRIEAGRAAWLEEVR
                     WSPLQQRQ"
     gene            5446..6024
                     /locus_tag="P5316_15920"
     CDS             5446..6024
                     /locus_tag="P5316_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRR86497.1"
                     /translation="MRQLIRIAPVAGLFALWCAGLAGCTGFDLPSSDGGDWWDPGDGG
                     GGGGGVESGYCEPVGAPDTAVQQLMFEAINTYRLANGLDELLYSDTLEEAADFQAQDM
                     YARSFFDHTNPDGEGPMDRAVAAGFCRPRLVGENIAYGQQSVNEVQVGWQNSPGHNAN
                     MLRADFVFVGMGHYTAPIGTQYWVQLFGTPFE"
     gene            6073..6651
                     /locus_tag="P5316_15925"
     CDS             6073..6651
                     /locus_tag="P5316_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRR86498.1"
                     /translation="MMRLLTGLLWRTSAVVLLVSLPACVGGLPLPGIGGFPDVLGDDA
                     NDPGAGASGYCEPIGTPDTSTQQQMLNALNNYRLANGLGILMYSDSLEQAAQAHAQDM
                     HARNFFDHTNPDGKTSFDRAVAAGFCSPLMVGENIAYGQQSVADVQAGWEGSPGHNAN
                     MLHTGYAFVGMGHYVSFLGEHYWVQVFGTTFE"
     gene            6846..10553
                     /gene="carB"
                     /locus_tag="P5316_15930"
     CDS             6846..10553
                     /gene="carB"
                     /locus_tag="P5316_15930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRR86499.1"
                     /translation="MPKRQDLKCVMIIGSGPIIIGQGCEFDYSGTQACKALREEGIRV
                     VLLNSNPATIMTDPEMADRVYIEPITPEAIDKVLAMEKARGTPVDALLPTLGGQTGLN
                     CAVRAADAGILGKHGVQLIGADQKAIHKAEDRTEFKNAMLRIGLDVPRSGIAHSWQEA
                     REVLKEIGLPVVIRPAYTLGGTGGGFATTPTEFETICKRGLEYSLNSEILIEESCLGW
                     KEYELEVMRDRADNVVIICSIENFDAMGVHTGDSITVAPAQTLTDKEYQRMRDAAIKI
                     IREIGVETGGSNIQFAVHPKTGRMVVIEMNPRVSRSSALASKATGYPIARIAAKLAIG
                     YTLDEIPNDITRKTPASFEPTIDYCVVKIPRWTFEKFPEADETLTTQMKSVGEAMSIG
                     RTFKEALQKGIRSMEIKRFGLGLDANDKWLAAMQASESAWRREAETPGEPEPLKGWRK
                     GEAPADTVEQSAQKYPIPFPVLRDKLSRPCQGRLYYIRYALKMGWSVEKIYELSHIDP
                     WFLENIKELVEFEDELITLAQEGRDDLKRLFEDCARAQAEYDQHGMRRSPATDDVAAR
                     LSAGSSLAVFERLRQVVRQAKQWGYSNVQLATVFNVPHVQVRAFCRYGGLEPVYKLVD
                     TCAAEFEAETPYYYSSHENPPATLGKMDVTSVEHGGNRQCSERLVAFAEHDDEIRVSE
                     RQKVIVLGGGPNRIGQGIEFDYCCVHAVFTARDMGYETVMINSNPETVSTDYDTSDLL
                     FFEPLTHEDVLNICERLNGRPFIPLTPPSPPCQGGARGGGLVKGVIVQFGGQTPLNLA
                     QGLKDAGVPILGTSPESIHLAEDREAFAGVLNELGLRQPENGIAYDLEGAKEIARRIG
                     YPVLVRPSYVLGGRAMEKCHLEEDLVRYMDKALQATDRMAGSGKAHPILIDRFLSEAT
                     EVDVDCICDGKKAVVCGVMEHIEEAGIHSGDSACALPPYALSPAIIDEIKQQTERLAM
                     RIGVCGLMNVQYAVKDDVVYVLEVNPRASRTVPFVSKATGVPWAKLATEVMLGKSLDQ
                     VMRQRGLTDAPWPRHISVKESVFPFSKFPGVDCVLGPEMRSTGEVMGIDYSFGLAFAK
                     SQMAAGTALPTSGTVLISVNDHDKPLIVPIAREFKEMGFRIVSTRKTRDTLLAAGIEA
                     DLVSKIQDPAGPYLIDMINAGQIGLLINTPVFFGSAATESRIRSAAVMHNIPLVTTMT
                     GARAVVQAIRALREGDWTVRALQDYHRQPVG"
BASE COUNT         2032 a         3351 c         3378 g         2124 t
ORIGIN      
        1 ggcgggcagt gaccagtcgg catggctgaa tgtcagttga cacagatgca ggtcgtggac
       61 gggtttgccg gccttgcggt cacccgccac gcggatcagt tggggcaacc gcggggcgat
      121 cacagtcgtg ttctcgggcg tctcgccggg cttgggcacg tagtagagca tgccggtgga
      181 gcggtcgacg taccattgcc cgggcgtgtc cagggcctcg aagacgtttt cgacgtagta
      241 gcgggcaaac tgcttggggc tgtgcgcgtc ggtcagccgg aagatgctct tgcgggtgaa
      301 cttggcgagg ttctcggtct cgttgacctc ggccaacggc aggcgcgact cgatccagaa
      361 gtgcagggca acgacctcga cgtcaccgag gttccgccaa ttcctgatgt cgccgggctt
      421 gaaagtggct tcggtctgac cttcgttcca tggagtcttg tcggtcatct gcggcaggcc
      481 ggtgagctcg aagtaaccct ccttcggaag ccgcgttcgc ggccgacgct cgttgttgac
      541 aaaaagctgg cgagggtacc actttccggc ctttacgtcc gccaattccg cggcccagac
      601 cttcttgccg ttcacttcgg cggtcttcca gccggtgatc cgccggccgc cgctgatggt
      661 tgcgtgctca ttgccgaagc aagcgtacat gaccggggtg tcgcgtgtgc cggagtcctc
      721 agggccgaat accagtggct cctccagctc gtatgtcccg ggtttgacag atacgagcca
      781 cgcggctgcg ttcttcggct tctcggtcgt gcggatgcgt ctgagggcat cgcgcgcggc
      841 ttggatggtc ggcaaaggtc cgtctgtctt gtcgctgttg ggctcgcgga gccggcccga
      901 ccattccagt cggccgtcgg gcgagacata gatatctgca tcgatcatgt cctccaaagc
      961 gcgcgccacc gctgtctgaa ccgccggccg gaagacctct tccgatatca actggccgca
     1021 gccggccggg agtgtcgcca tgccaagtgt cgaggcgagc agggtcgtcg ttccacagag
     1081 gcgtgcgaag cggtgagagc gacgcgcagt ccaagacgac tcacgacggt tgaagacgct
     1141 ggctaccatg ttcatgctcc tgcgttgaag gtccggtgaa gtactcaccc acgagctcct
     1201 gcgtgtctcg ttgctctgtt aaggccggct atcatggccc caaggtgtcc tgccggcaag
     1261 tccgggccga ccgatcgcgg ggcgtcgagg agccaagacc gtgatcggga acctgacccg
     1321 gtgagtgtgg gtccggcatc ttgccggtca tgtgacgcgt caactggcag ggcagcgtcg
     1381 agcggagcga gacctgccgg tgcgcgacgt tggtgtgatt cacgggtgtg atcgtgttca
     1441 gcgtacaggc ggcaggtctc catcgccttc agtgattccg accatgccct acccttgcca
     1501 gatcagttga agcagttgtg ccggcgcatc gatcaggtga gcagcccctg cggcgatgag
     1561 ttcctcgcga tcacgatagc cccaggtggc tccgatggga acaactccgg cattccgggc
     1621 agtggcgatg tcggtgttcg agtcgccgac gagcatcacc tcgtttggct tcgccttcat
     1681 cctcgccacg atgtccagaa gcgttcttgg atcgggtttg cgacggtctt cttccttata
     1741 cccctcgatg gccaccagcg gccagcggcc gaagagcccc tgcgtcatcg ggacggtgta
     1801 cacgtgcggc ttgttcgaca agacgcccat cagcacgcct cgctttgcca gctcatccat
     1861 cagttccgca atgcccggaa acggcaccgt cttgtccatc tggtgctcgc ggtaatgctg
     1921 cgagacggcc gccatcatgc gttcgaaggt cgccttgtcc gcgtccggca atgcccgccg
     1981 gcacagagtc ggcaatccct cgccgatcca acgacgtacc tcgtcgcggg atgcctcggg
     2041 cctcccgact gcggcgagtc cgtagttgat ggcgtcggca atcgccggca gcgtgtcggc
     2101 cagcgtgccg tcaaggtcga aaacgacgcc cgttgtgttc atcctcgtga attcactcaa
     2161 ctctggttct atggctcgct gcccaatctg aagacatgga gatgaagctc cgtgcctcac
     2221 ccgaagacct aggggatgaa cccctcggct cgtcaagatc gcggctacga gcctctccca
     2281 ccgaatcctg aaccctgaac cctctactgc accttctgtg ccgcctcgct cggtgtgtca
     2341 acgaacttca ccggtgttcc ggccttgacc atcttggcca gccgtagcgc atcccagttg
     2401 gtcagcctga agcagccgtg agaaccggtc ttgccgatca tttcgggatt tggcgagccg
     2461 tgcatgccca cgcccggcag ggtcaactgg atccaacagc ggccgaccgg gtttcgcggc
     2521 cctggcggaa tggtcagctt gctggtgatc ttctcggtca cctcaggcca catcttggga
     2581 tcgaacgtgt actccggatc ggtggcgctg aactcgacct cggcactgcc cgacggcagc
     2641 ttggccttgc tggccgcgat ggagcagtga aacagccccg ccagttgctt gtcacgatca
     2701 ataacccgaa tgatcttgtc gttgaggttg acttcgagtt gtgtcgcctg cggcagacgt
     2761 gcggaactgg cgatggccgg gccgatgtac ttgtcgccgg gcttgaggtt gctgagtttc
     2821 ttgcccgggt tgagccgctg gagcagtcct cgcgtgcagt ggcatttctc ggccagagcc
     2881 tcttccagat cgctgtagcc caacaggctc ttcttgctct tggccaccca gctatggggt
     2941 accggatcaa cctccttaag atcggccgat tcgatggtgt agaggatgag cgccccatcc
     3001 ggatcgacct tgagagcctc ggcggtcgcc ttgtccaatt caccggtctt gggcagtccc
     3061 ttaacccgct gaaattccca ggtggcgatc ttcgtcttgc gtccggggtt accgtcgatc
     3121 aatccgggtg agaagccgac gttttccagt gcaacctgcc aggcgatatt ccgccgaagg
     3181 cttgactcgc tttcctgagc gatggcgggc ctgagtgtca cacagccgct gatgcaggcg
     3241 gttgcgatca gaaagtggta tactgtcccg acatatctgt acgtttgagg aagcatgtga
     3301 cctccttgtc ctcgcgcgat cgcaactatt ccctgcgggc cggggcggtt gctggggcgt
     3361 caggcgattg tatctgaccg gccactgaag ggccagccat cgatcgggcc caactctcat
     3421 attcggcaag acaatcctcc ggactgatga acttcttcca gactgcccgg gcgcggtaac
     3481 cgtattcacg gtcctgttcg acggggtgga tgacatactg gaaatcccag gccggccggg
     3541 ggaacttatc gagcttgaat ttgaacaggc tgaaccgcag ctcgtcggcc ggggtatacg
     3601 cccggtcgaa catgagaata tatgtcatgc ccttggctgc ccgaccatag tagaagggct
     3661 tgctgaaccg ggggtactcg tagctccaac tgttgagccg aaacctcaga tcgtcgtcgc
     3721 ggggcaaatc ctttgcctgg acgtgccgcc acgttccgcc atgattgtgg tcgggatggc
     3781 cgggcggggc atcgacccgg atccattcct cccgaccgtc tcgccgttcg atcccgcgga
     3841 agttaatcgc cacgtcttcg acgtcgttca tgtagttggc ccagaagaga atcgcgtacc
     3901 gatgtctgcc gaacagcgag gcgtcggccg gacggcatct gaactcgaaa tcaatgtagt
     3961 ggggcggcgt caccgtgtat ttcatcgtac tggccatggc ccaggggtca tctgccgcat
     4021 tccgcaccag catgaccgat cggccgtccg gcaacgtgta caggcgataa ggcccgtgtc
     4081 gcggagtgaa agcattgtgc gggttctcat gtcccgcaat gatgtgctcg aagttcaaac
     4141 cggccgccgc tcccttgctg tcagggtcga aggcgttgaa gcccggcgcg tccttcacgt
     4201 tgatgagcga cgcaatgccg ctgagcacgc cgggcgactc ggagttgtcc agaaagacga
     4261 cgtcgagatc gccggcgcgt atgtgggcgg atttcgccga atcgccggcg gggcgggtcg
     4321 agacctcact cgcagggtcg gcctcacaac agcataggac aaccagcatt tggagcaaca
     4381 tgtcagccct cccgacgtcc ggccgattca ttccctcatc gcataaccat gctatcacac
     4441 ggccgtgaat cggacgacgg gcggaacgct ccatctccac ctgaaatcaa acacccgtgt
     4501 caagtcttgc tcctggaatc cgctgcgata caatcctacc catgcgtcag ggacaagaag
     4561 gtgagggcaa ggggacttgc gacgccctga tgggccgcga ccaggcagtc agtcgccgcg
     4621 aactgctggc gtcgctcgcc gtggcggtct cggcccgggc ggccttggcg gcggtcggca
     4681 cttcacggcc cgcaagccga ccggcagaca cgataccgtg ggacgacccg ccgactgatg
     4741 cagtcctcgg tcgagtcctt tacgacggta cgcctccggt tcttgcgccg gtcgactgta
     4801 gcgccgaccc caactgcgcc gccctctacc gccacaaccc gctcctgccg gaggacctgg
     4861 tggtgagtaa gaccggcggg ttggctcatg tcttcgtgtc ggtgaaacac gggttggatc
     4921 cgggtcgccg atggcccgtg cccgatcgac cggtgatcct ggatcaaaag ggctgcctct
     4981 acattcccca cgtgttcggc gtcatggccg gccagccgct ccagatcctc aacagcagca
     5041 agatcaacga ggttccgcac ggatacccca aacgtaaccc ggagttcagt ttcactctgc
     5101 ccaagcgcgg catgagtaag accgtcgtgc tcaacgagcc ggaagtgttt cgcatcggct
     5161 gtgacgtgca tccgtgggaa acggcgtggt gccacgtgat ggcccacccg ttctttgcgg
     5221 tgaccgacac cgaggggcgg ttcatgatcc gcggtctgcc ggccggcgag tacgaattgg
     5281 agttatggca cgagcatgcc acgctgggta cgctgaccag gagcgttcgc atcgaggcgg
     5341 gtcgggcggc ttggcttgaa gaagtcaggt ggtcgccgtt gcagcaacgg cagtagcgtg
     5401 gaaacagaga atcgcgtctt cttgggcgtc tggagaggtc gacagatgag gcagttgatt
     5461 cgcattgcgc cggtagcggg gctcttcgcc ctgtggtgtg cgggcttggc cggctgcacc
     5521 ggcttcgatc tgccctcttc tgacggtggc gactggtggg atccgggcga tggtggagga
     5581 gggggcggcg gcgtcgagtc cggttattgc gagcctgtcg gagcgcccga tacggccgtt
     5641 cagcaattga tgttcgaagc gatcaataca tatcgactgg ccaacggtct tgacgaactg
     5701 ctctattccg ataccctgga agaggctgcg gacttccagg cccaagacat gtatgcccgt
     5761 agcttcttcg accataccaa ccccgacggc gagggcccga tggaccgcgc ggtcgcggcc
     5821 ggattctgtc gtccgaggct ggtcggtgaa aacatcgctt atggacaaca atcggtgaac
     5881 gaggtgcagg tgggctggca gaatagtccc ggccacaatg ccaacatgct cagggccgac
     5941 ttcgtctttg tcggcatggg acactacacc gccccgatcg ggacgcagta ttgggtccag
     6001 cttttcggaa cacccttcga gtaggcctcg cggggggttg ttcggcgggc atgtgcccgc
     6061 gggagaacaa agatgatgcg attactgaca ggcttgctct ggcggacctc ggctgtcgtg
     6121 ttgcttgtgt ccttacccgc ctgtgttggc ggtcttccgc tgccgggtat cggagggttt
     6181 ccggacgttc ttggtgacga tgccaatgat cccggcgcgg gcgcatcggg ctattgcgag
     6241 ccgatcggta ccccggacac gagtactcag cagcagatgc tgaatgcttt gaacaattat
     6301 cgtcttgcga atggacttgg gatactgatg tattccgact cgctcgaaca ggccgcacag
     6361 gcacacgcgc aggatatgca tgcccgcaac ttcttcgacc ataccaatcc cgacggcaaa
     6421 acctcattcg accgcgctgt ggccgccggc ttctgcagtc ccttgatggt cggcgagaac
     6481 atcgcttatg gccagcagtc cgtggccgac gttcaggccg gttgggaagg cagcccgggt
     6541 cacaacgcta atatgctcca cactggttac gcgtttgtcg gcatggggca ctatgtctcc
     6601 ttcctggggg agcattactg ggtacaagtc tttggcacca ccttcgagta gacatgcccc
     6661 aaaggcaccc tcgcccaacc ggtcatggat ccctactggc cggcgttccc gccgcgagtc
     6721 gctttcgttg agagtttcgg ctatcctttg gctttcaacg gttatcgccg gacgcctgaa
     6781 acgggccgct tgcggcgcgg catggggatc cctgtgatgg gccggcgacc gatcgggaag
     6841 cgtaaatgcc taagcgacag gatcttaagt gtgtaatgat catcggctcc ggcccgatca
     6901 tcatcgggca gggctgtgag tttgattact ccgggaccca ggcatgcaag gcccttcgcg
     6961 aggagggcat tcgcgttgtt cttctcaaca gcaacccggc gaccatcatg accgacccgg
     7021 agatggccga ccgggtctac atcgagccga tcacgccgga ggccatcgac aaggtcctgg
     7081 caatggaaaa ggctcgcggc acgcccgtag atgcgctgct tcctaccctc ggcggccaga
     7141 cgggtttgaa ctgcgcggtg cgcgcggcag atgcgggcat cctcggcaag cacggcgtgc
     7201 agctcatcgg cgcggatcag aaagccatcc acaaagccga agaccgtacc gaattcaaga
     7261 acgcgatgct ccgcatcggt ctcgatgtgc cgcgatccgg gatcgcccat tcgtggcaag
     7321 aggcccggga ggtgctcaag gagatcggct tgccggtggt catccgcccg gcatacacac
     7381 tcggcgggac cggcggagga tttgccacca ctcccacgga gttcgagacc atctgcaagc
     7441 gcgggctcga atactcgctc aatagcgaaa tcctcatcga agaaagttgc ctcggctgga
     7501 aggaatacga actcgaggtc atgcgcgacc gcgcggacaa cgtggtcatc atctgctcca
     7561 tcgagaactt cgacgccatg ggcgtgcaca ccggcgattc aatcaccgtt gctccggccc
     7621 agacgctgac cgacaaggaa taccagcgca tgcgggacgc ggcgatcaag atcatccgcg
     7681 agatcggcgt cgagaccggc gggtcgaaca tccagttcgc cgtgcatccc aagaccggcc
     7741 gaatggttgt gatcgagatg aacccgcgcg tctcgcgaag ctccgccctg gccagcaagg
     7801 ccaccggcta cccgatcgcc cgaatcgccg caaaactcgc catcggctac accctggacg
     7861 agatccccaa cgacatcacc aggaaaacgc cggccagttt cgagccgacc atcgactact
     7921 gcgtggtcaa aatcccacgc tggacgttcg agaaattccc cgaggccgac gagaccctga
     7981 ccacccagat gaagagcgtc ggcgaggcca tgtccatcgg ccgcacgttc aaagaagcgc
     8041 tccagaaggg cattcgttcg atggagatca agcgcttcgg cctgggcctg gacgccaacg
     8101 acaagtggct ggcggccatg caggcctccg aaagcgcctg gcggcgtgag gccgaaacac
     8161 ccggcgagcc cgagccgctc aaaggctggc gcaagggcga agctccggcc gacaccgtgg
     8221 aacagagtgc tcagaagtat ccgattcctt tcccggtact tcgcgacaag ttgtctcgcc
     8281 cgtgccaggg ccgcctctac tacatccgct acgcccttaa gatgggctgg agcgtcgaga
     8341 agatctacga actcagccac atcgatccat ggttcctgga gaacatcaag gagctggtgg
     8401 agttcgagga tgagttgatc acgctcgctc aggagggcag agacgatctg aagagactct
     8461 tcgaagattg tgcccgagct caggccgagt atgaccaaca cggcatgagg cgctcgccgg
     8521 caaccgatga tgttgctgcc agactctctg ccgggtcctc tcttgcggtg tttgagcggc
     8581 ttcgccaggt cgtccgtcag gccaagcaat ggggctactc gaatgtgcaa ctggcgacgg
     8641 tattcaacgt tccccatgtg caggtgcgtg cgttctgcag atatggcggc cttgagcccg
     8701 tctacaagct ggtagacacc tgcgcggcgg aattcgaggc cgagacaccg tactactact
     8761 cgtctcacga gaatccaccg gccacgttgg gcaagatgga tgttacctcc gtggagcatg
     8821 gaggaaatcg gcaatgctcc gagcgtcttg ttgctttcgc tgaacacgac gacgaaatcc
     8881 gcgtcagcga acggcagaaa gtcattgtac tcggcggtgg gccgaaccgt atcgggcagg
     8941 gcatcgagtt cgattattgc tgcgtgcatg ccgttttcac cgcccgcgac atgggctatg
     9001 agacggtgat gatcaactcg aaccccgaga cggtctcgac tgactacgac acgagcgacc
     9061 tgttgttctt cgagccgctg acgcatgagg acgtattgaa catctgcgaa cgtctgaacg
     9121 gcaggccgtt catccctttg acccccccgt cccccccttg ccaagggggg gccagggggg
     9181 gtgggctcgt caaaggtgtc atcgtccagt tcggcggcca gacgccgctg aacctggccc
     9241 aggggctcaa ggatgccggc gtgccgatct tgggcacctc gcccgagagt atccacttgg
     9301 ccgaggaccg cgaggccttt gccggcgtgc tcaacgaact gggtctgcgc cagccggaga
     9361 acggaatcgc ttatgacctg gaaggtgcca aggagatcgc cagacgcatc ggctacccgg
     9421 tgctggtgcg gccgtcgtac gtgctgggcg gtcgggcgat ggagaagtgt cacttggaag
     9481 aagacctggt gcgttacatg gacaaggccc tgcaagccac cgaccgtatg gccggcagcg
     9541 gcaaggctca ccccatcctc atcgatcgat tcctcagcga agccaccgag gtcgacgtcg
     9601 actgcatctg cgacggaaaa aaagcggtgg tttgcggcgt gatggagcac atcgaggagg
     9661 ccggcatcca cagcggcgac agtgcatgcg ccctcccccc ctacgcgctg agcccggcca
     9721 tcatcgatga gatcaagcag cagacagagc ggctcgccat gcggatcggt gtctgcggcc
     9781 tgatgaacgt gcaatacgca gtgaaggatg acgtggtcta cgtactggag gtcaacccgc
     9841 gggcctctcg aaccgtgccc ttcgtcagca aggccaccgg cgtgccctgg gcaaaactgg
     9901 ccaccgaagt aatgctcggc aaatcgctcg accaggtgat gaggcagcgc ggcctgaccg
     9961 atgctccctg gcccagacac atttcagtca aagagtcggt cttcccgttc agcaagttcc
    10021 ccggtgtgga ttgcgttctc ggccccgaga tgcgcagcac cggcgaggtt atgggtatcg
    10081 actactcgtt cggcctggcg tttgccaagt cgcagatggc ggccggcacg gccctgccaa
    10141 caagcggcac cgtgctcatc agcgtcaacg accacgacaa accgctgatc gtgccgatcg
    10201 cccgtgagtt caaggagatg ggctttcgga tcgtctcgac ccgcaagacc cgcgatacgc
    10261 tgctcgccgc cggcatcgag gcggacctgg tatcgaaaat acaggatccc gccggtccct
    10321 atctgatcga catgatcaat gccgggcaga tcggcctgct gatcaacacc ccggttttct
    10381 tcggcagcgc agccaccgaa agccgcatcc gctccgcggc cgtcatgcac aacatccctc
    10441 tggtcaccac catgaccggc gcccgggcgg tcgttcaggc catccgggcc ctccgcgaag
    10501 gcgattggac cgtccgggca ctgcaggact atcaccgtca gccggtcggc tgaccgtgcg
    10561 gtccgggcgg cgaggggctc gtcatctgag gctgagctgc cgggaaaacc gtgcttgttg
    10621 agcgaggtct tattctcccc cgccccgcat cccacgtccc atgccgacat gttgtcggcg
    10681 cacaaacgcg tatcgccggg atgaaacacc cctcagggca tctcatcccg gcgatcccgc
    10741 gggaggagga aagacgaaag aaagtacttg ctgtctgtat gctctgccct acatctgatt
    10801 atacgcctgc cggcggcctc ggttcgcatt cttccacaat aatctggtcc aatataaagc
    10861 ctgcccgaga gctgtggcgc gtgtt
//
LOCUS       DAOTPW010000194        10872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5676_length_10872_cov_5.161690, whole genome shotgun sequence.
ACCESSION   DAOTPW010000194 DAOTPW010000000
VERSION     DAOTPW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10872
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5676_length_10872_cov_5.161690"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="P5316_15935"
     CDS             <1..1091
                     /locus_tag="P5316_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRR86500.1"
                     /translation="TSYNAYLVRGNSATALIDSADPALQEELMRELEGLGHLDYVISQ
                     HSEQDHSGAIPVVLKRFPNATLVCSTKARDLLTTHLHVPDEHIRTVTDGQTIDLGGKT
                     LQFVETPWVHWPETICTYIPEDKCLFSCDFFGSHLATTDLYVTDQARVYEAAKRYYAE
                     IMMPFRKVITRNLEKIRALDFRKIAPSHGPVYDKPEFIIEAYQDWVSPTMKNEVVLPY
                     VSMHGSTAAMAKHLVAALADRGVTVHVFDLAVADIGKLAIALVDAATLIVGTPTVHVG
                     PHPAVHYAAHLTDLLRPKLKYAAIIGSYGWSSKAIEQITGLIPNLKVEVLGTVLHKGM
                     PTAETFAQLDKLADAVAKAHHQDELVRT"
     gene            complement(1450..2274)
                     /locus_tag="P5316_15940"
     CDS             complement(1450..2274)
                     /locus_tag="P5316_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86501.1"
                     /translation="MKKPSLFAAASAIIVVTILAVPAPMLAAPLLPGGMLYPVPGEPD
                     PVGGEVIAGAVAVPLVAATFSGSATTMVIAGDASNPLGGLTFTYQVTNHPGSANVITQ
                     LGLVGFAGLMVDASYQVPGEGRPPASISLSSTGDGIFFTFLAGIGPGALDPGSSSAVL
                     VLQTNASNYCRTKLSAIDGSVGEAASYAPAVAADFDHSGLVDSTDFEIFDACATGPGV
                     PYRPGHLPPDCILTPDAQDKIAADFDADGDVDQSDFGLFQQYLNAPQAPEPSDRPE"
     gene            complement(2674..3273)
                     /locus_tag="P5316_15945"
     CDS             complement(2674..3273)
                     /locus_tag="P5316_15945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86502.1"
                     /translation="MASGAFLPVGGTLYPAPLELDPLPGLVLFTTGPLPFVGGAFSGA
                     LESVVIAGDPTNPWGGLTFAYMVSNNAGSTNDIGRLTVIDYTGWQTDASYQAPGTSMP
                     PLPLGGAVPPAYINRSTPDVVGFSFAGFPLGPGVLRPGMSSTWLVVQTDAPRWVHTTA
                     YVIDGSIASVPSVGPIPEPAALALLGLGTAFVLRRRRHA"
     gene            complement(3614..4609)
                     /gene="rlmN"
                     /locus_tag="P5316_15950"
     CDS             complement(3614..4609)
                     /gene="rlmN"
                     /locus_tag="P5316_15950"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRR86503.1"
                     /translation="MDVLGMTFDEVRSAVGARVHEIKALRAEYRAALAGHGGDRMRAD
                     LLPVVRQTEDGDLIKFVQRTHDGLETESVMVPMRRHGREWKSLCVSSQIGCARGCVFC
                     ETGQMGLVRNLSVSEIVGQVVAVRTHFGQKVRNIVFMGMGEPFDNFDNVVRAVAVLND
                     PAGLSISCERIAISTVGRTEGIRRLAAMGRRRINLAISLNAPNDEIRSRIMPVNRLEP
                     MDKLREVLLAYPLRRCQFLMIEYVLIPGVNDQREHALQLAEYLRPLKCVVNVIPYNPR
                     HESPWPAPDEQIVRQFIVWLKEEGQVVKRRLTKGREHMAACGQLGNREMVRGRRG"
     gene            complement(4649..5002)
                     /locus_tag="P5316_15955"
     CDS             complement(4649..5002)
                     /locus_tag="P5316_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86504.1"
                     /translation="MMHAKGRRLAPVLVFAMLAGASGYIWWLTSPREFRGKLLHGPGM
                     AGIPEDPPPLAYLVMADGSHELDIPRHMADRDFRRRLMEMDGKQVIVRGKRAIRRTML
                     RRHRAIRVLDLRLCE"
     gene            complement(5505..5873)
                     /locus_tag="P5316_15960"
     CDS             complement(5505..5873)
                     /locus_tag="P5316_15960"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRR86505.1"
                     /translation="MSDDEFELVVDEAIQSIPEGFHRYLQDIAVDIEEMPDEDTCREM
                     GIGDPRSLLGLYRGTPLTERHVEDPYRYPERIVIYKQNIERMCKDRRRMIRQIRKTVL
                     HEVGHHFGLDERQLRELGYD"
     gene            5989..6996
                     /gene="sppA"
                     /locus_tag="P5316_15965"
     CDS             5989..6996
                     /gene="sppA"
                     /locus_tag="P5316_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRR86506.1"
                     /translation="MNHNHNVRAVVLTLFTLSLAAMTGCGPQSFKITPVPADRTLEES
                     VVLDEGGLLTPKIALIDVDGIIMNRQQSGLFSDGEHPVSLLIEKLDKAAKDKSVKALI
                     LRINSPGGSVTASDIMYSEVLRFKKETKGKRPVVAVLSDVAASGGYYVACGADRIVAH
                     PTTVTGSIGVIMQMINFAGTMSKIGVKADAITSGKMKDAGSPLREMRPEEREVFQSLV
                     DQFYDRFVKVVADGRPKLTEEQVRTIADGRVWSAQQALELGLIDEIGTLRDAVAGVKE
                     QVGLKKVKVVTYHRPLNWKPNIYAESPPAQPQVNLINVTVPPTWVRPEPQFMYLWVPE
                     P"
     gene            7690..>10872
                     /locus_tag="P5316_15970"
     CDS             7690..>10872
                     /locus_tag="P5316_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR86507.1"
                     /translation="MSGILCIAPISQAALTVGVSSPMHKVMIAGQHKGWPFEPYEGTF
                     ASTYSLALARAEHEAFQVVVIPDQNVTNASVTVSSLQPTSGQGAFNGQVSVWLVGHVK
                     GAAQPRSDLYIEYPPYLVNYNLTDSGGWWPDPLLTFKNTCNINAGDRVAFWVNVATTN
                     TTPPGDYTATVTVSAAGQTPVTLSLNVRVWNFTLPARSSLPTAFSMDSLWQASWIYGG
                     SWSGTIQNQYYQMQQAHRMSVTEIYNAAPKSPGWFTPWLNLNNAFCLSKVPTQSASGL
                     ATLYNYFISLGRLNETYVYGYDEAPSDKFQEMYTTFSGIHSDYPGLRTMTTAYDSSFG
                     TSPTTSFLRDVVDIWVPGTVTYNRTAAQALRAEGKDMWWYVAEYPRHPTGANWLLEYP
                     FIEARLLMGAMTFKYNPGGFLYYAVTNYGHGLYAAGLMSKNGLIESGPYTNWDARTLY
                     SEKYNGFTDADGCLYYPGPVNVGPLPSIRSQNIRDGLEDYEYLVLLKDIAGKLGRCPS
                     TPEVDAYVQSARALLAVPAEVVAGLSTSAYTRDPAVLYGYRQQLAEAIVQGQALLLVM
                     PVPPDSDGDGVGDSCDNCPNVHNPDQANTDGDSLGDACDADIDGDGILNAQDNCPYVH
                     NLSQLDSDGDGIGNACDNCPDVNNPDQLDSDGDGRGNRCDKCPSVYDPDQLDSDGDGV
                     GDLCDNCPTVHNPDQADDDEDTIGDLCDPDPLGGRRLDERFDGMLTGDKKIGSWNLSS
                     MQARWPLTYTYAGSTGGTFATNTGLEPGGGAMNTTKTSYRMTANLEPNMSGTYGEGNV
                     GIGTGKVLFGTDDKPLTLEFTVDFRGETYGSRSNFYVELSLDAEQAPRQGMTSEDPDL
                     GNGDQGPWRADRVHNVLAIGSFTAVNRDPVAALSAGTMGAACYFDGLRWHYSKQMYDL
                     DGNPVSLWKSSFGGLTTFKMIIRSHTVTIQLTNPDDTPTVRGPHTVPRVYTGGFNRVS
                     LTMGNPLSDAARYSFVDNISVRNGLILDPTQTGACCLPDQTCSEILSVSCATAGGSYQ
                     GDGTSCDSNPCAPPVLVVNAGPDKEINLGGR"
BASE COUNT         2236 a         3608 c         3088 g         1940 t
ORIGIN      
        1 gcaccagcta caacgcttat ctggtacggg gcaacagcgc gaccgcgttg atcgacagcg
       61 ccgacccggc cctccaggaa gagctgatga gagagctcga aggcctgggg cacctcgatt
      121 acgtgatctc gcagcattcc gagcaggacc attcgggcgc catacccgtc gtgctcaagc
      181 gatttcccaa cgccacactg gtctgcagca ccaaggcgcg agatctcctg accacccatc
      241 tgcacgtgcc agacgaacat atccggacgg tcaccgacgg ccagaccatc gacctgggcg
      301 gaaaaacgct gcaattcgtc gagacaccct gggtccattg gcccgaaacg atctgcacct
      361 acattcccga ggataaatgc ctgttctcct gcgacttttt cggctcgcac ctggcgacta
      421 ccgacctgta tgtcaccgac caggcacgcg tctacgaggc cgccaaacgc tactacgcgg
      481 aaatcatgat gcccttccgc aaagtgatca cccggaacct ggagaagatc cgtgccctcg
      541 attttcgcaa gatcgccccg agccacggcc cggtctatga caagcccgag ttcattatcg
      601 aggcctatca ggattgggtg tcaccgacaa tgaagaacga ggttgtcctg ccctatgtca
      661 gcatgcacgg aagtacggca gcaatggcca aacacctcgt cgccgccctg gcagatcgcg
      721 gcgtcactgt ccatgttttc gatctggcag tcgccgacat tggcaagttg gcaatcgccc
      781 tggtggatgc ggctacgttg atcgtgggca cgccgaccgt gcacgtcggc ccacaccccg
      841 ccgtccacta cgccgcgcat ctgaccgatc tgcttcgccc caagctcaag tatgccgcaa
      901 tcatcggctc ctacggctgg agcagcaagg cgatcgaaca gatcaccggc cttattccaa
      961 acctgaaggt cgaggtgctc ggaacggtat tgcacaaagg aatgccgacg gcggagactt
     1021 ttgcgcagct cgataagctg gccgacgccg tcgccaaggc tcatcaccaa gacgaactgg
     1081 tccgaacgta gaccagccgg ctcacgtcga acggcatagg gctatacccg ctgccgagac
     1141 tcagatcgaa gaggcctagg cgccggggaa tcgaggcttt ccagcgtcct tttcgggttg
     1201 ccgactcttg ccaattcggt ggctgccttc ggcggctttc ggccgttgca gccgaagctg
     1261 cggcgtccca cgggaaggct gcccgccggc gcacaactga ccgaccggaa acgcccctcg
     1321 catcgggcga acacggacgc ggtaagacgc cgtaccttcg ccgcgcagcc cgcgactcgg
     1381 acctgccgct cgacttgtcg gcatccggca cggctccgcc gccgcccgga agatcggcgg
     1441 ctcgattcct tactctgggc gatccgacgg ttccggcgct tgaggggcat tcaggtattg
     1501 ctggaacaga ccgaagtcgg attggtcgac atccccgtcg gcgtcgaaat cggccgcaat
     1561 cttgtcctgg gcgtccggcg tgaggatgca gtccggcgga agatgccccg gacgatatgg
     1621 cacgccggga ccggtcgcac acgcatcaaa gatttcgaaa tcggtcgaat ccaccaagcc
     1681 actgtgatcg aagtccgccg ccacggcagg ggcgtaactc gccgcctcgc ccacggaacc
     1741 gtcgatggcc gagagcttcg tcctgcagta attcgacgca ttcgtctgca acacgagcac
     1801 ggcgctgctg ctccccgggt cgagtgcccc aggaccaatt ccggccagga atgtaaagaa
     1861 gattccgtcc ccggtgctgg acaagctgat actcgccggc ggtctgccct cgccgggtac
     1921 ctggtagctc gcgtccacca tcaatccggc aaagccaacc agccccaact gagtgatcac
     1981 gttggcgctg ccgggatggt tggtcacctg gtaagtaaat gtgagcccgc cgagaggatt
     2041 tgaagcgtct ccggcaatga ccatcgtggt cgcgctgccg ctgaaggtgg ccgctacaag
     2101 aggcaccgcg accgcgccgg caatgacctc accgccgaca gggtccggct cgcccgggac
     2161 aggatacagc atcccgcccg gtagcaaggg tgccgcgagc atgggcgccg gaacggcaag
     2221 aatcgtgacc acgatgatgg ctgatgcggc tgcaaacagg gatggcttct tcatgatctt
     2281 tccctcctgc ccggtagatg gtcccggggt ctcctcgtca ggatgactga acgctaaacg
     2341 gcagcgtaga ggcaatcgtc gcgcgggaca ggcatcttgc cgaccaaacg atgaacccgt
     2401 ccctccgcct tagagagccc tacgctgcac cctggtatga tacctcaccc cccaggccgc
     2461 gtcgagaccg tttggggcgt ctggaagaag ccgcgcgacc tgtagcgggc gccacccgcc
     2521 actctcgccc tgggcagggt acaccttcac cgggttgggc ctttttccgt atgaaccgtt
     2581 cactcctttt tcctcgacga agccgtacat cgaaaaaaaa gccgccaccc ctgggggcgg
     2641 cggctgaata acctttacga atgtcggcaa aaatcaggcg tgcctgcgcc gacggagcac
     2701 gaaagccgtg ccgagcccca gaagggccag cgcggcgggt tcggggatcg gtccaacgct
     2761 gggaacgctg gcgatcgaac catcaatgac gtacgccgta gtatggaccc atctcggggc
     2821 gtccgtctgg acgacgagcc acgtgctgct catgcccggt ctcagtacac ccgggccgag
     2881 cgggaagccc gcgaaactga aaccgaccac atccggcgtc gaacgattga tatacgccgg
     2941 aggtaccgcg ccgcccaaag gcagtggggg cattgacgtt cctggcgcct gataactcgc
     3001 atcggtctgc caacccgtgt aatcaatgac ggtaagacga ccgatgtcgt ttgtgctgcc
     3061 cgcattgttg ctcaccatgt aggcaaaggt gagcccgccc cacggattcg tgggatcacc
     3121 ggcaatcacc accgactcaa gcgcgccgct gaatgcaccg cctacaaacg gcagcggacc
     3181 agtggtgaac aggacaaggc ccggcagtgg gtccagttcc agcggtgccg ggtacagggt
     3241 cccgccgacc ggcaaaaacg cacccgaggc catgggcacg ccacaaacca aaacaacaag
     3301 cgcagataca aaaccgagtc gactcgtaga cgtgtgcatt ttccacgctc ctatgcttta
     3361 cccctgcaat tccccacgct acgacgactc cgccgtagct tttccagtgc ttcatggtat
     3421 ggcagccctc cccgttttgc aaggggcatt ccgaaaaaaa cctagctttt ggggtcgctg
     3481 ggccgataac gacattagtt attctcctta aaggttttat gatcgccttg ctgcagcgct
     3541 gtacctgtcc cgggccagtc cgttgatgga ttatcggcca tcgaggggga ccccggccgg
     3601 catgatgagg gcgctagccg cgccggccac ggaccatttc gcggttcccg agctgtccgc
     3661 acgcagccat gtgttcgcgg cccttggtga ggcgccgttt cacgacctgc ccttcttcct
     3721 tgagccagac aatgaactgc ctgacgatct gctcatcagg tgccggccag ggcgactcgt
     3781 gccgcgggtt gtacggaatg acgttgacga cgcacttgag cggtcgaagg tactccgcca
     3841 gttgaagagc atgttcgcgc tgatcgttga cgccagggat caatacatac tcgatcatga
     3901 gaaactggca cctacgcaac gggtaggcca acaggacctc gcgaagcttg tccatgggtt
     3961 cgagtcggtt caccggcatg attcgcgagc ggatttcatc gttcggagca ttgagcgaaa
     4021 tcgccagatt gatccgacgc cggcccattg cagccaatcg tctgatcccc tcggttcgcc
     4081 cgacggtgga gatcgcgatc cgctcacagg aaatcgacag ccctgccgga tcgttcagca
     4141 ctgcaacggc ccggaccaca ttgtcgaagt tgtcaaatgg ctcgcccatg cccatgaaga
     4201 cgatgttgcg gaccttctgc ccgaagtggg ttctcaccgc gaccacctgc ccgacaatct
     4261 cggacaccga cagattacgc accagaccca tctggcccgt ctcgcagaac acgcaaccgc
     4321 gggcgcaacc gatttgcgat gagacgcaca gcgacttcca ttccctcccg tgccgccgca
     4381 tgggaaccat gacgctctcg gtctccaagc cgtcgtgcgt gcgttgcacg aacttgatta
     4441 aatcgccgtc ctcggtttgt cggactaccg gcagcaggtc ggcccgcatc cgatcacccc
     4501 catggcccgc cagcgccgcg cggtactcgg cccgcagcgc cttgatctcg tgcacccggg
     4561 caccaaccgc cgaccgaacc tcgtcaaacg tcatccccag aacgtccacc agccggtctc
     4621 ctttcgttga tcactgaacg attccggtct attcgcacaa tctcaaatcc agaacgcgga
     4681 tggcccggtg tctccttaac atggtacggc gaatcgcccg ttttccccgc acgatcactt
     4741 gcttgccgtc catttccatc aaccgccgac ggaaatcccg atccgccatg tgtcgaggta
     4801 tgtccaactc gtggcttcca tccgccatga ccaagtaggc cagagggggc gggtcctccg
     4861 gaattccggc catgcccggt ccgtgcagca acttgcctcg gaactcgcgc gggctggtca
     4921 gccaccagat gtagccgctt gcgcctgcca acatggcgaa aacgagcacc ggcgcaagcc
     4981 gtcgtccttt tgcgtgcatc aacgcgcctg tgatcccctg ccagtcgggt cacgactgaa
     5041 gaccgatcgc tctcacacgg gcaatgagcc ggcgacagag cgaaagcaca tctcttccca
     5101 tcctactctg ctcctggacc gaaaccgagt gggcgttccc ccccgacagt tgaggcgagc
     5161 tcaccgtgcg aaggcgggga caggcagcca ttccggcgcg ccgccgcccg atcccgatac
     5221 gaccacctgt atgagatgct catgatcaac tacatggtgg tcgccgacgc tctccgtccg
     5281 ccgcccgccc gggcaacctt gacgtaccag acgccgacga atcggatcga ggagcatccg
     5341 ggggagacgt accagccggc catgacgtac agggcaaggc gattgagctt tgtggcagat
     5401 ccattctcgc aagcgacggc cggacaggat cgtcggaccc gccctgagag caatacccga
     5461 agtcgcgacc ggcacaccgg catggggcgg caccccaatc gacgtcaatc gtaccccagt
     5521 tcgcgaagct ggcgctcatc cagaccgaag tgatgaccga cctcgtgcag aacggtcttg
     5581 cggatctgac ggatcatacg cctgcgatcc ttgcacatcc gctcgatgtt ttgtttgtag
     5641 atgacgattc gctcgggata gcgataggga tcctccacat gccgctccgt cagcggtgtc
     5701 ccccgataca agccgagcag cgaacgggga tccccaatgc ccatctcgcg gcaggtatcc
     5761 tcatcgggca tctcctcgat atctaccgcg atgtcctgaa gatagcggtg aaacccttcc
     5821 ggaatcgatt ggatcgcctc gtcgaccacc agctcgaatt cgtcatcgct catccgcaca
     5881 gacagcctcc ctccgggata ctggctcgcc aaccgcgcag gctctacaat atcgaccggc
     5941 agaaccgacg ttctccaccg ccgatttcat ggaggagtat agcccgtaat gaaccacaat
     6001 cacaacgtcc gagctgttgt cctgacgctc ttcacgctgt ccctcgccgc catgaccggc
     6061 tgcggcccac agtcgttcaa gatcactccc gttccggccg accggacgct ggaagagtcc
     6121 gtggtattgg acgagggcgg tttgttgact cccaagatcg ccctcatcga cgtcgacggg
     6181 atcatcatga accgccagca gagcggcctt ttcagcgacg gcgagcatcc cgtctcgctg
     6241 ctgatcgaga aactcgacaa ggcggccaag gataagtccg ttaaagcgct catcctgcga
     6301 atcaacagcc ccggcggcag cgtgaccgcc agcgacatca tgtactccga agtgctgcga
     6361 ttcaagaagg aaaccaaggg caagcgtccc gtggtcgcgg tactttccga cgtcgccgca
     6421 agcggaggtt actatgtcgc ctgcggcgcc gaccgcatcg ttgcccaccc gaccaccgta
     6481 accggaagca tcggcgtcat catgcagatg atcaacttcg ccggcacgat gagcaagatc
     6541 ggtgtgaaag ctgacgcgat cacgtctgga aaaatgaagg atgcgggttc gccgctccgc
     6601 gagatgaggc cggaagaacg cgaggtgttt cagtccctgg tcgatcagtt ctacgaccgc
     6661 ttcgtcaagg tcgtcgccga cggccggccc aagttgaccg aagagcaagt gcgaacgatc
     6721 gccgacgggc gggtctggtc cgcgcagcag gctcttgagc tcggactgat cgacgagatc
     6781 ggaacgcttc gcgacgccgt tgccggcgtg aaggaacagg ttggactcaa gaaggtgaag
     6841 gtggttacct accaccggcc gctcaactgg aaacccaaca tttacgccga gtcgcctccg
     6901 gcccagcctc aggtcaatct gatcaacgtg accgtgcccc cgacatgggt ccgacccgag
     6961 ccacaattca tgtacctctg ggtcccagaa ccatgatatg acggagctcg ggcttaccct
     7021 ttgttatgca ggcagcccca ggcagttcgc cgcagagagg tcatcgcggc gtgcctcgac
     7081 gggacgatgc cgactgcagc gcttcaacaa cggagccttc agacgtctca gcggcctgaa
     7141 tccgcggctc ggggcgactg aggcccactt gctcgctgtg aacctgctga acctgaccca
     7201 gacaccaaac ggtcactccc accagtgcgc ctgccaacaa catgcgacgc cacttcacag
     7261 cagtcccctt tcctcgaacg acctcggcag agcggatcgc gcccggccga aagtcctatt
     7321 atcggaatat ggggtatcct ccttatagtt ttcttcctgt caatagcccc cttcgaccgg
     7381 accgcacgcg cggcccgtgg cacttgaggc ggctcttcac tttcggaacc gcgaccgtac
     7441 ctgtccggcg taccctccgg ccaagccaac atggcccccc tgcggttcgc aggaaaaacg
     7501 ggtgggcgcg agagggggca acgccataaa atggggaaac gccgtccgga accgttgccg
     7561 gccaaggcgg tccgggggga ttcccggata cgcggggcca tgtggccgtg ttgcggattc
     7621 cataccaacc agataactga ttgatcggtt gggggggtta tgcaaacgca tcgatttgtt
     7681 gtggcagcga tgtcagggat cctgtgcatc gcgccgatca gccaagccgc tttgaccgtc
     7741 ggggtctctt cccccatgca caaggtgatg atcgcaggcc aacacaaggg ctggcccttc
     7801 gagccttatg agggaacgtt cgccagcacc tacagcctgg cgctggcccg agcggaacat
     7861 gaggccttcc aggtcgtcgt catccccgac cagaacgtca ccaatgcgag cgtcaccgta
     7921 tcgtcgctcc agccaaccag cggccaaggc gccttcaatg ggcaggtgag cgtctggctc
     7981 gtcggccacg tgaagggtgc cgcccaaccc cggtctgacc tctatatcga gtaccctccc
     8041 tacctggtca actacaacct caccgacagc ggcggctggt ggcccgaccc cctactgact
     8101 ttcaaaaaca cgtgcaacat caatgccggc gaccgcgtgg cgttttgggt caacgtcgcc
     8161 accaccaaca ccacgccgcc aggcgactac acggccacag ttaccgtctc cgccgccggc
     8221 cagacgcccg taaccctgtc gctcaacgtg cgggtctgga atttcaccct tccggcccgg
     8281 tcctcactgc cgacggcctt ctccatggac agcctctggc aggcaagctg gatctacggg
     8341 ggcagttggt caggcacgat tcagaaccag tattaccaga tgcaacaagc ccatcgaatg
     8401 agcgtgacgg agatctacaa tgccgcgccg aagtcccccg gctggttcac gccctggctc
     8461 aacctgaaca atgccttctg cctcagcaag gtacccacgc aaagcgcgag cggtctggcg
     8521 accctgtaca actacttcat cagtctggga cgactgaacg agacatacgt ctacggctac
     8581 gacgaagcgc cgtcggacaa gttccaggag atgtacacaa ccttttccgg tatccactct
     8641 gattaccccg gtctccgcac gatgaccaca gcctatgatt ccagtttcgg cacctctccg
     8701 accacctctt tcctgagaga cgtcgtggac atctgggttc ccggcaccgt cacctacaac
     8761 cgcacggcgg cacaggcgtt gcgggccgaa ggcaaggaca tgtggtggta tgtcgccgaa
     8821 tatccgcgtc acccgacggg ggccaactgg ctgctcgaat accccttcat cgaggcccgt
     8881 ctgctgatgg gggcgatgac tttcaagtac aaccccggcg gtttcctcta ctatgcggtg
     8941 accaactacg gccacggtct gtacgctgcc gggctcatga gcaagaacgg actgatcgaa
     9001 agcggaccct acaccaattg ggatgcccgt actctctaca gcgagaagta caacggtttc
     9061 accgacgccg acggatgtct ttactacccc ggaccggtga atgtcgggcc gctgccgtcc
     9121 atccgttccc aaaacatccg cgatggtctc gaggattacg agtacctggt cttgctcaag
     9181 gacatcgccg gcaagctggg acgctgcccg tcaacacccg aggtcgatgc ctacgtgcag
     9241 tcggcccggg cactgctcgc cgtgcccgcc gaggtggttg caggcctgtc aacctcggcc
     9301 tacacccgtg acccggcagt cctctacggc taccggcaac agttggccga ggcaatcgtc
     9361 caaggccagg ccctgctgct tgtcatgccg gtcccgcccg actcggacgg tgacggtgtg
     9421 ggcgactcct gtgacaactg ccccaacgtt cacaatcctg atcaggccaa cacggacggt
     9481 gatagtctgg gcgatgcctg cgacgcggat atcgacggcg acggcatcct caacgctcag
     9541 gacaactgcc cgtatgttca caatctcagc caacttgact cagacggcga cggcatcggc
     9601 aatgcctgcg acaactgccc ggacgtgaac aaccccgacc agctcgactc agacggcgac
     9661 ggccggggca accgctgtga caagtgtccc agcgtatatg atcccgacca gctcgattca
     9721 gacggcgacg gcgtgggcga tttgtgcgac aactgcccaa ccgtgcacaa cccggatcag
     9781 gccgacgacg acgaggatac catcggcgat ctgtgcgatc ccgaccccct tggcggccgc
     9841 cgactcgacg agcgtttcga cggcatgttg accggcgaca agaagatcgg ctcctggaac
     9901 ctgtcgtcaa tgcaagcccg ttggcccctg acctatacct acgccggctc aaccggcgga
     9961 acgttcgcga cgaacacggg cctcgaaccg ggcggcggcg caatgaacac gacaaaaacg
    10021 tcgtaccgca tgacggccaa tctcgaaccg aacatgtccg gcacctatgg agagggtaac
    10081 gtgggtatcg gcacgggcaa agttctcttc ggcaccgacg acaagccttt gacgctcgaa
    10141 ttcacggtgg actttcgcgg tgaaacgtac ggcagccgtt caaatttcta cgttgaactc
    10201 tcgctcgatg ccgaacaggc ccctcgccaa ggcatgacct ctgaggatcc ggatctggga
    10261 aacggagacc agggcccctg gcgggcggac cgggttcaca acgtcctggc gataggctct
    10321 tttacggccg tcaaccgcga tccggttgcc gccttgagcg ccggcaccat gggcgcggcc
    10381 tgctacttcg acggcctccg atggcactac agcaagcaga tgtacgacct tgacggcaac
    10441 cccgtcagct tgtggaaaag ctcctttggc ggactgacga ccttcaaaat gatcatccgc
    10501 tcccacacgg tcaccattca gcttaccaac cccgacgaca ccccgaccgt gcggggcccc
    10561 cataccgtcc cccgcgtcta caccggcgga ttcaaccgcg tcagcctgac catgggcaac
    10621 cccctgagcg atgccgcccg ttacagcttc gtggacaaca tctcggtgcg caacggcctc
    10681 attctcgatc cgacgcaaac cggggcctgt tgcctgccgg atcagacgtg cagtgaaata
    10741 ctgtcggtca gttgtgcaac cgccggcggc agctatcaag gcgacggcac gagctgtgac
    10801 tccaatccgt gcgccccgcc ggttcttgtt gtcaacgccg gaccggacaa ggaaatcaac
    10861 ctcggcggcc gg
//
LOCUS       DAOTPW010000195        10803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5726_length_10803_cov_4.739766, whole genome shotgun sequence.
ACCESSION   DAOTPW010000195 DAOTPW010000000
VERSION     DAOTPW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5726_length_10803_cov_4.739766"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..2941)
                     /locus_tag="P5316_15975"
     CDS             complement(257..2941)
                     /locus_tag="P5316_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86508.1"
                     /translation="MGTLGPWTFQSAAQSLPASTATTTATSQPDRNVEPIERPAIAGR
                     RVVKHFDFDEQRFGNYDPYPMYWKQHEAAGFPLYLEGKFDANEGHDRAPSFRLDLNGG
                     SIAFHYVGRDISVRTNSDYLVVAWCKTSPLTWARAYVTARYLDRKGRVIEGTQRCSEL
                     LGGTGEPQEWVPLMIGLPGNVPDSRYIGISVWLTQASTWNTNARPMRSVEFQEIKGSA
                     WFDDITVYRLPRVCLRTSRPGNVFTEMEPVTLHPEVSDPDGLNLSAKLAIRDAEGQVV
                     EERPVSVRTTHMTEPQAAVYRNLPVGAYTAELDVVSDDGADLVRRKLGFVRLASQITP
                     PTESGRGFGIVLQDMSLGVVAGQRDLLLNLHPELVKVPVWWSGQITGSTEQDLAIDHY
                     LEMIAETQADPVGILANGCPPGAQRPDEQTRSMLEIFSEDPIGWKPLIAGLWSRYAGL
                     IHVWQVGADGDASTFLDHKYPRILPALVREMKSLMTDPLLASTINTKYVPPAEPLASY
                     NAVLLSADVPLDDVERHLAPFLDKERSRTWITVEAPPEGRYPRLQRLSDLSRRLVEAH
                     FQKTGGVFLTAPWAAHADLLSAQVDPSEDYVVFRTIADLLGGTEPVSRGSLDGQAKCM
                     LFDRNGRAIMMVWDEYAPPGGREHTLWLGENAEQVDLWGVRRPLQPLGKYRLVSIGPV
                     PTFIINVPTWLMEFCRQFVLAPPMVEFSFDAYERDVIFRNTYHEPISGLLRLLTPPDW
                     DVRPNRIPFALQPNEVLRSPVQIRFPINAEAAVTPLVGEFEIDADQRYQIVTPAWFEL
                     GLENIDVNVNVYRLGTRVVVQQTMTNRTDQPVSFEGYVVAPARKRMGRRFINIVSGQS
                     ARKEFLIDNAGDLVGKRIRVGLNELQGTRIWNRVITVP"
     gene            3163..4800
                     /gene="nadB"
                     /locus_tag="P5316_15980"
     CDS             3163..4800
                     /gene="nadB"
                     /locus_tag="P5316_15980"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRR86509.1"
                     /translation="MTELYDTRRYLTQFDVRRIPNLFADVLVIGSGVAGLRAAIEAAK
                     YAPVLLVTKGRVDESNTARAQGGIASVMAPDDTFDAHIEDTLRVGCGLCDRAAVERIV
                     REGPSRIQELIEWGAIFDRRQSMLSLGLEGGHSAPRIVHAMGDATGLEIANTLIRVAR
                     ASEQIRIFEDCFTIDLLVDEGRCCGVTTFHRHFGHQIFWAKQVILASGGYGQVYREST
                     NPPITTGDGHAMAYRAGVPLRDMEMVQFHPTTLYVAGATRALISEAVRGEGAYLVDRE
                     GRRFMEAYHPDMELAPRDVVSRAILSEMARQNVTCMYLDVGHLNVDRFRKRFPTICRL
                     CEEFDIDISKDRIPVRPAAHYMIGGLVVDENTRTPISGLLACGEVASSGVHGANRLAS
                     NSLLEGLVFGRVAGTVAGEAAAGTNGPAQPKLIRSEVERSQRTSLDLADVLNSLRSVM
                     WRNVGIERTGARLAETEEIIQFWGRYMMDKLFDDQLGWETQNLLTVARLMTASALARQ
                     ESRGVHFRTDYPNTDPNWNGRHVIVERCGGKLKVDVQ"
     gene            complement(5341..6405)
                     /gene="mtnA"
                     /locus_tag="P5316_15985"
     CDS             complement(5341..6405)
                     /gene="mtnA"
                     /locus_tag="P5316_15985"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRR86510.1"
                     /translation="MPDLAPHRTLDWIGGLDGHIEMIDQTLLPGRFSVIACHDVESVW
                     EAIRVLRVRGAPAIGVAAAMGAVLGVRDATTDEDLYARLQRACDYLASSRPTAVNLFW
                     ALDRMKRRAQTLRGRPLADIKESLLAEARAIRDEDAAMCRAIGDAGRHLITDGAGVLT
                     HCNAGGLATAEYGTALAVIYRAHELGRRFRVYADETRPLLQGSRLTAWELSRAGVDVT
                     VICDNTAGLLMAQGKINLVITGADRIAANGDSANKIGTYSLAVLAKAHRIPFYIAAPR
                     STFDLSIDDGPAIPIEIRDENEIRRGFGRLTAPEDVKCYNPAFDVTPAGLIHGIITDR
                     GLIRPVTPEQVRTTIGRPGG"
     gene            complement(6398..6907)
                     /gene="purE"
                     /locus_tag="P5316_15990"
     CDS             complement(6398..6907)
                     /gene="purE"
                     /locus_tag="P5316_15990"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRR86511.1"
                     /translation="MSDATVAIVMGSDSDWPVMEACYQQIRSLGIAVVVEVISAHRTP
                     DRVTEFVAAAEQRGVRVFIAAAGMAAALPGVVAAHTTLPVIGVPLPSGVLQGVDSLLS
                     IVQMPPGIPVATVAIGSAGAKNAALLAAEILALSDPRLKKALADLRREQAAAMEQKNR
                     SLKEKLAGA"
     gene            7717..8544
                     /locus_tag="P5316_15995"
     CDS             7717..8544
                     /locus_tag="P5316_15995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HRR86512.1"
                     /translation="MTRKSRPNEVSYGAKPGIDDTKAVWREFLKNRRREYRNLLVERY
                     APLVQMQAARLTRKLPAHVTYEELCSAGYDGLIEAVEAYNPNKKAKFETFCQQRIIGA
                     IMDWLRSLDPQSRTIRAFEKRCCQVTEILESRLARPPTQEEVAEAMHLSVERYSELSR
                     ISQRGREVHFSALEPRGGAGLNRQTGRRVWDIGDERQRDPSERLSRAMLIDLITRGLT
                     REERLVLILYYCEDLTMAEIGAVLDLSESRVSQIHKDLLERLRKRYKNRLAQGQLVA"
     gene            9186..10181
                     /locus_tag="P5316_16000"
     CDS             9186..10181
                     /locus_tag="P5316_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRR86513.1"
                     /translation="MAVRNSGTIVGPALPNIPWEDRPPGCGEVVWRYSRNPVIPRDLI
                     PCANSIFNSAVVPYRDGFAGVFRVDDRRRYMRLHSGRSSDGINWRLNPEPIGFLGQDP
                     EVCRFEYGYDPRVCRIDDRYYVTWCNGYHGPTIGMAYTQDFETFHQCENITLPYNRNG
                     VLFPRRINGKYYMLSRPSDTRHTSFGDIFCSESPDLVYWGRHRFVMGTRKGWQSTKIG
                     AGPVPIETQHGWLLIYHGVLTSCNGYVYSAGAALLDLDEPWRVIYRAAPYILSPQRDY
                     ECVGDVPNVVFPCAALADADTGRIAIYYGCADTVTGLCFAQVDELIEFMKCHSEV"
BASE COUNT         2130 a         3132 c         3394 g         2147 t
ORIGIN      
        1 ccggttcaca accacggccg gcatcggcgg cgcgacaaga ggaccgctcc ccgccggcca
       61 gacgatcctg gtcacgccgt cgctgaccat ctcagcccaa acccggaacg caccacctga
      121 ccctgatcga tgctcagttc gtcgacacga tccatgtgac gtaccccgcc tcgaccggcc
      181 acccgctgga gattgcggtc ggaccataag ggtccgaaag agcgggtgca accagaggag
      241 atggtgtcgg tgttgctcag ggcacggtga tgacgcggtt ccagattcgg gtaccttgaa
      301 gctcgttgag gccgactcgt attctcttgc cgaccaggtc gccggcgttg tcgatcagaa
      361 actctttgcg agcgctctgc cccgagacga tgttgatgaa gcggcgaccc atgcgttttc
      421 gggcgggggc gacgacgtat ccctcaaagc tgaccggttg gtcggtgcgg ttggtcattg
      481 tttgctgcac cacgacgcgg gtaccgaggc gatagacgtt tacgttgacg tcgatgtttt
      541 cgagcccaag ctcgaaccat gccggcgtga cgatctgata tcgttggtct gcatcgatct
      601 cgaactcgcc gaccaagggt gtgaccgcag cctcggcgtt gatcgggaaa cggatctgta
      661 ccggtgaccg taacacctca ttcggctgca gagcgaaggg gatgcgattg ggtcgcacgt
      721 cccagtccgg cggggtgagc aggcgcagca gaccgctgat cggctcgtga taggtgttcc
      781 gaaagatcac gtcgcgttcg tatgcgtcaa agctgaactc caccatcggc ggagccagca
      841 cgaactggcg gcaaaactcc atcagccagg ttggaacgtt gatgatgaac gtcggcaccg
      901 gaccgatgga cacgagccgg tacttgccca gcggttgcag cggccgacgc acgccccaca
      961 ggtcgacctg ttcggcgttt tcgcccagcc acagcgtatg ttcccgcccc cccggcgggg
     1021 cgtattcgtc ccaaaccatc atgatcgccc ggccgttgcg atcgaagagc atgcatttgg
     1081 cctgcccgtc gaggctgccg cgtgaaacgg gttccgttcc acccagcaaa tcggcgatgg
     1141 tgcggaacac cacatagtcc tcactcgggt cgacttgcgc ggacagcagg tcagcgtgtg
     1201 ccgcccaggg ggcagtaaga aagacgccgc ctgttttctg aaaatgagcc tcgacaagac
     1261 ggcgcgacag atcgctgagt cgctggagac gcggatagcg accttccggc ggagcctcga
     1321 cggtgatcca ggttctggag cgttccttgt cgaggaaggg ggcaagatgt ctttcgacat
     1381 catcgagcgg cacgtcggcc gaaagcaaaa cggcgttgta agacgccaac ggctcagcgg
     1441 gcggcacata ctttgtgttg atcgttgagg cgagcagcgg gtcggtcatc aagcttttca
     1501 tctcacgaac cagagcgggc aggatccgcg gatacttgtg atccaaaaac gtcgacgcgt
     1561 cgccgtcggc gcccacctgc cagacgtgaa tcaagccggc ataacgcgac cagagacctg
     1621 cgatcagcgg cttccagccg atcggatcct cgctgaagat ctcgagcatg gagcgagttt
     1681 gttcatccgg tctttgggca ccgggcggac aaccgttggc cagaatgccc acgggatcgg
     1741 cctgcgtctc ggcgatcatt tccagatagt gatcgatcgc cagatcctgt tcggtggagc
     1801 ctgtgatttg gcctgaccac cacaccggca ccttgacgag ttcaggatgc agattcagaa
     1861 gcaggtcgcg ttgtccagcc acgacaccca ggctcatgtc ctgcaagaca atgccgaatc
     1921 cccgaccgct ttcggtcggg ggagtgattt gcgatgccag tcggacaaag ccgagcttgc
     1981 gacgcaccag gtcagcccca tcatcggaaa caacgtcaag ttcggcagtg tatgctccca
     2041 ccggcagatt tcggtagacg gctgcttgcg gctcagtcat gtgggtcgtc cgaaccgaaa
     2101 cggggcgctc ttcgaccact tggccctcgg cgtccctgat ggccagcttg gccgacaggt
     2161 tcaagccatc cggatcgctg acctccggat ggagcgtcac cggttccatc tcggtgaaca
     2221 cgttgccggg tctgctcgtt cggagacaga ctcgaggcag gcgataaacg gtgatgtcgt
     2281 cgaaccaggc agatcctttg atctcctgaa actcaacaga ccgcatgggt cgggcgttgg
     2341 tgttccaagt gcttgcctgg gtcagccaga ccgatatccc aatgtagcgg gagtccggca
     2401 cgttgccggg caggccgatc atcagaggca cccattcctg aggctcaccg gttcctccaa
     2461 ggagctcact gcaccgctgc gttccctcga tcacgcggcc tttgcgatcc aggtaacgag
     2521 cagtgacgta tgctcttgcc caggtcaaag gtgacgtctt gcaccaggcc actacgaggt
     2581 agtcgctgtt tgtgcgaacc gatatatcgc gcccgacata atggaacgcg attgacccgc
     2641 cattaaggtc gagtctgaag ctgggcgcac gatcgtgccc ttcattggcg tcgaacttgc
     2701 cttcaagata gagtgggaaa cctgcagcct cgtgctgctt ccagtacatc gggtacgggt
     2761 cgtaattgcc gaagcgctgc tcgtcgaaat cgaaatgttt gaccaccctt cggccggcaa
     2821 tcgccggacg ttcgatgggc tcaacgttcc tatccggctg ggacgttgcc gtggtggtcg
     2881 ctgttgaggc cgggagagac tgagcggcag actgaaacgt ccagggcccc agtgtcccca
     2941 agactgtaat ccatactagt gcctttttcg gcaagtcagt tatgcaccgg cgggcaatac
     3001 gccagtggca tctccagagt gccgcacaga cccgctcatc ggaccggcgc gcagcataac
     3061 ctctcctcga tggttcctac cgaagttatc ggctgatcca gcggcgagaa tcagtcttgg
     3121 gcattgaagt cagagcccca ccgacttagc atatgttccg aaatgacaga gctatacgac
     3181 acaagacgct atctgactca gtttgacgta aggcggattc ccaacctgtt tgccgatgta
     3241 ctggttatcg gtagcggtgt ggccggcctg cgggcggcca tcgaggccgc caaatatgct
     3301 cccgttcttc tggtcaccaa ggggcgggtt gacgagtcca acaccgccag ggctcagggt
     3361 ggcattgcgt cggtcatggc ccctgatgat accttcgacg cccacatcga ggacacgtta
     3421 agggtcggtt gcgggttgtg tgaccgcgca gcggtcgagc ggatcgtccg cgaaggaccg
     3481 tcgcggattc aggagctgat cgagtggggg gcgatattcg accggcgaca gagtatgctg
     3541 agccttggct tggagggcgg gcattcggcc cctcgtatcg tgcacgcgat gggcgacgca
     3601 acgggcctgg aaatcgccaa cacgcttatc cgcgttgcca gggcttccga gcagatccga
     3661 atctttgaag actgtttcac cattgacttg cttgtggatg agggtcgttg ctgcggcgtc
     3721 acaacgtttc atcgtcactt cgggcaccag atcttctggg ccaagcaggt cattctggcc
     3781 agcggcggtt atgggcaggt ttaccgcgaa tcgaccaatc ccccgatcac aacgggtgac
     3841 gggcatgcga tggcgtatcg tgccggcgtt cccttgcgcg acatggagat ggttcagttt
     3901 catccaacga cgctctatgt ggccggggcg acgcgggcgc ttatcagcga ggccgtgcga
     3961 ggcgaaggcg cttatcttgt cgatcgcgag ggacgtcgct ttatggaggc gtatcacccc
     4021 gacatggagc tggccccgcg cgacgtggtc agccgggcta ttctcagcga aatggccaga
     4081 caaaacgtca cctgcatgta cctggacgtc ggccacctga acgtggacag gttccgcaag
     4141 cgattcccca ccatctgtcg cttgtgcgag gagtttgata tcgacatcag caaggatcgc
     4201 ataccggtgc ggccggcggc acattacatg atcggcggct tggtggtgga cgaaaacaca
     4261 cgcacgccga tatctggcct gctggcatgt ggcgaagtcg ccagcagcgg cgttcacggg
     4321 gccaatcggc tggccagtaa ttccctgctc gaagggctgg tgttcggccg ggtcgccggg
     4381 acggtcgccg gcgaggcggc cgccgggact aacggaccgg ctcaaccaaa gctgattcgc
     4441 agcgaggtag agcggtcgca acgaacttcg cttgacctgg cggatgttct gaattctctc
     4501 cgcagcgtga tgtggcgcaa tgtcgggatc gaacgtacgg gcgcccgact cgccgagacg
     4561 gaggaaatca tccagttctg gggtcgatac atgatggaca aactgtttga tgaccagctc
     4621 ggctgggaga cgcagaacct gctcacggtc gcccggttga tgactgcctc agcactggcc
     4681 cgccaggagt cacgaggtgt ccactttcgt accgactacc cgaatacgga tcccaattgg
     4741 aacggccgac acgtcatcgt tgagcgatgc ggtggaaagt tgaaggtgga tgtgcagtga
     4801 agccgacaga gtcttggctg ccgatccgtg tcgacgcgcc ttcctgtgcg gagctgccga
     4861 cgtttcctga acccattcgc gggtgcttct atggcgacca tgtgcagcag tgagatgaac
     4921 acggcacgat cagcaacccc tccgcgacca cacggttcca cgtagagccc gttcttgacc
     4981 cgcaagacca attcaggatg acggacatcg tagcgatccc cgctgctggt agcggtgcgg
     5041 aaagactcaa acggcttgcg cctcaagaaa tcgtcaatgg cgtcaagcgc cacaacagga
     5101 tcattgtagc tcagctcgat gccaagatcg caggctatct tgtcaagtct cggggatgag
     5161 aaacaagatg ggattcgccg gaagggtacc gacataccag accgtgctgg tgttcgttgt
     5221 gccggttttg gcgcctttgc cgatgttgtt gcgcatgtta cggagttggg gacggtcagt
     5281 tattcccggg cttgccggaa gggcacgctg cttcgcaggg cagtagcggg ccttggttag
     5341 tcatccccct ggtcggccga tggttgtacg gacttgttcg ggggttaccg gtcggatgag
     5401 gccgcggtcg gtgatgatgc cgtgaatcaa cccggcaggc gtgacgtcga aagcgggatt
     5461 gtagcacttg acgtcttcgg gggcggtgag cctgccgaaa ccacggcgga tctcattctc
     5521 gtcgcgaatc tcgatcggga tggccgggcc gtcgtcgatg gagagatcaa aggtcgatct
     5581 cggggccgcg atgtagaacg ggatgcgatg ggccttcgcc agaaccgcga gggaataagt
     5641 gccgatcttg ttggccgagt cgccgttggc ggcgatgcgg tcggcgccgg tgatgaccag
     5701 gttgatcttg ccttgagcca tcagcaggcc ggccgtgttg tcgcagatca cggtgacgtc
     5761 gacgcccgct cgcgacagtt cccaggccgt gaggcgggaa ccctgcaaca gcggccgtgt
     5821 ttcgtcggcg tacacgcgga acctgcgacc gagttcgtgt gcccgataga tcaccgccag
     5881 ggcggtgccg tattcggcgg tggcaagccc gccggcgttg cagtgcgtca ggacgccggc
     5941 tccgtcggtg atgagatgcc ggcctgcatc accgatcgcc cggcacatgg cggcatcctc
     6001 gtcgcggatc gcgcgagcct cagccaaaag tgattccttg atgtcggcga gcggtcggcc
     6061 ccggagcgtt tgagcgcggc gtttcatccg atccaaagcc cagaacagat tgacggccgt
     6121 gggacggctg gaggccagat aatcgcaggc tctttggagc cgggcgtaca aatcttcgtc
     6181 cgtcgtcgcg tcgcggacgc ccaggaccgc gcccatggcc gcggccacgc cgatagccgg
     6241 cgctccgcgg actctcagga ctctgatcgc ctcccaaacc gattcgacgt cgtggcaggc
     6301 gatgactgag aaccgtccgg gaaggagcgt ctgatcgatc atctcgatat ggccgtcgag
     6361 gccgccgatc caatcgagtg ttctgtgagg ggcaagatca ggcaccggca agtttttcct
     6421 tgagactgcg gttcttctgt tccatggccg cggcctgttc gcgtcgcaaa tcagccagag
     6481 ccttcttcag acgcgggtcg ctcaacgcca ggatctccgc ggccaacagg gcggcgtttt
     6541 tcgcccccgc agaaccaatg gcgaccgtgg cgacggggat accgggcggc atctgcacaa
     6601 tgctcagaag gctgtccacg ccttggagga caccagacgg caagggcacg ccgatcaccg
     6661 gcagggtggt gtgagcagcg accactccgg gcagagccgc ggccatgccg gcagcggcga
     6721 tgaacactcg cacgcctctc tgctcggcgg cggccacaaa ctccgtcacc cggtcgggcg
     6781 tccggtgggc ggagatgact tccacgacca ccgcgatccc cagcgacctg atctgctggt
     6841 aacaggcctc catcaccggc cagtcagagt cgctgcccat cacgattgcc accgtcgcgt
     6901 cactcattct cttcggctcc acaagagaca gaccgacctt gtcggcgagc ttaccagcgg
     6961 tggatcattg taatcgccaa ttggcgcctg ggaagccgta aggtggttgc cggcgtcgaa
     7021 ccgggccttt ccggggtgcg ggtgttcgtt tttgccacgc gcgggcggtc ctacggccgt
     7081 cggagggagg aaatctcagg gctttttttc gaggcgcgtt atcggccgct tattggaatc
     7141 tcccccaagc ggtcgtcggg gaagcctgcg tggaaaacca tatgtggggg ggctgtgatt
     7201 tccggcgacg cgtcccataa gccgagagcc taagcacttg cgttttcagg cctttgcgcc
     7261 gcttgctcat cgcaaaactc gtgctatttt taccacggtg ggcgagagat tgtgacttac
     7321 gtgcggtgcc aggatcgacc caatccaagg caccggttca aggaaggcat gtgattgaag
     7381 tcacgaatct gctcaccacc ttccctcgcc gtgtcacagg gacacgattc aatgttcgac
     7441 agtcggcagg aatcagcaca tcaggtgcga acgagtcgtt cgcatcgcga cctgagaagg
     7501 cgtcatgagc ttcaggcctg ccgctcgtcg gccttcggag actcaactga acgcatcgag
     7561 gttgatacgg gcgggaggcg aggtcgaacc ctgaggcaaa cggtcttctt gcggggtgca
     7621 acctgagcgc actgcgatcc ggaaacgtgc gcacaagccg cacagatgtc tggccgtagg
     7681 gttttaaccg ggcactcgtg gagcatagcc aacaatatga caagaaagag ccgaccaaac
     7741 gaagtgtcgt atggagcgaa gccggggatc gatgacacga aagcagtatg gagagagttt
     7801 ctcaagaacc ggaggcgcga atatcgcaat ctgctcgtgg agcgttatgc gccgctggta
     7861 cagatgcagg cggccaggct gacgagaaag ctgcccgcgc acgtgaccta cgaagagctc
     7921 tgcagcgccg gctatgacgg ccttatcgag gcggtagagg cttacaaccc gaacaagaag
     7981 gccaagtttg agactttctg ccagcagcgg attattgggg cgatcatgga ctggctgcgt
     8041 tcgctggatc cccaatcgcg gaccatccgg gcattcgaga aacgatgctg tcaggtgacc
     8101 gagatactgg aaagccgcct tgcaaggcct ccgacgcaag aggaagttgc cgaggcgatg
     8161 catctttccg tcgaaaggta ttcagaattg agtcgcattt cgcagcgagg ccgggaggtg
     8221 catttttccg cgctggagcc gcgaggcggt gccgggctta accgccagac cggcaggcgc
     8281 gtctgggaca tcggcgacga gcggcaacgg gatccttcgg agcgcctgtc tcgggcaatg
     8341 ctcatcgacc tgatcaccag aggtttgacc cgggaggaac ggctggtgct gattctgtat
     8401 tactgcgagg atctgaccat ggccgagatc ggcgcggtgc ttgacctatc ggaatcgagg
     8461 gttagccaga tccacaagga tctgctggaa cggctgcgga aacgatacaa gaatcggctt
     8521 gcgcagggcc aactcgtggc ctgaggcatg ccaaggcgga ccgccctttt tgcgggccgc
     8581 aacccaagcg ttctgcgatc cagaaacacg cgcgcaaaat gcgcaccagt tgcgacggaa
     8641 gagactaaag ggacattccg ggggaacgca ctcccttcga ctcttgtcgt atctcacgtt
     8701 gcgagttgcc ggggcaggcg aagccggctt gctctggcaa gacaaaaaaa cagagagcat
     8761 gatcggtccg acgagaactc gaagccgctg ctctctggtt ttcgcacttg ccgccagagt
     8821 tcgtcggagc acacactccg gcctcacaag cccatctgct gcggcgaacg ttgccggctg
     8881 catggcgaga cgacctttcc cctggtcgca ctcttattgt atcggacaga aagctccggt
     8941 tccagggatt atccgaaacg agagttcggc atggtagcgc ggttcgcatc ggagtgcggc
     9001 tcgacagggc tcaatcgccg atactgctct gcataagtct ttgcctaatg aacagttacg
     9061 ttccagttgg ccatacgttc aatgcgaagt cttgacgggc tctgtcgtcg tccggtaggt
     9121 ttgcggcccg atgcggagtg ctgggccgcc ttctcgcaga cccgtagtcg aaggagtatc
     9181 gaacggtggc ggtacggaat tcaggaacca tcgtgggccc cgctttgccc aacattccat
     9241 gggaggaccg gccgcccggt tgtggtgagg tggtctggcg ttacagccgg aacccggtta
     9301 tccctcgtga tctgattccc tgcgcaaaca gcattttcaa cagtgccgtc gtgccttaca
     9361 gggatggttt tgccggtgtc ttccgggtag acgatcgccg tcgttatatg cgtttgcaca
     9421 gcggtcggag cagcgatgga atcaactggc ggctgaaccc cgagccgatc gggttcctgg
     9481 gccaggatcc ggaggtttgc cgattcgagt acggctatga ccctcgcgtt tgccggatcg
     9541 acgatcggta ctacgtcacc tggtgtaacg gttaccacgg tccgacgatc gggatggctt
     9601 atacacagga cttcgagacg tttcaccagt gtgagaacat taccctgccc tacaaccgca
     9661 acggcgtgtt gtttcctcgc cggatcaacg ggaagtacta catgctgagc cgcccgagcg
     9721 acactcgtca cacgtcgttc ggcgacattt tctgcagcga gtcgcccgat ttggtgtact
     9781 ggggccggca ccgctttgtc atgggcactc gcaaaggttg gcagagcacg aagatcggcg
     9841 ccgggccggt gccgatcgag acgcagcacg gctggctgct gatctaccac ggcgtgctga
     9901 cttcctgcaa cggttacgta tacagtgccg gggcggcctt gctggatctc gatgaaccct
     9961 ggcgggtcat ctaccgcgcg gctccgtaca ttctttcacc gcagcgcgac tatgagtgcg
    10021 tgggcgacgt gccaaacgtg gtatttccgt gtgccgccct ggccgacgcc gacaccggcc
    10081 gcatcgccat ctactacggc tgtgctgaca ccgttacggg cctgtgcttt gcccaggtgg
    10141 atgagctcat cgagttcatg aagtgtcatt cagaggtata gccttgcctg tctggccgag
    10201 cggcggcggt ccggaggaag gccccgcggc ggaccgcctt cttgcgggct gcggcccaag
    10261 tgcattgtga tccggaaaca tgcgtgcaag ccgcacagat gtccggccgt aaggctgtaa
    10321 agacgccccg ccccggtctc caattcgatc cacctgaacc cttctagaag ggtgcacaag
    10381 aagcgtgacg gtcgcaaggg ggtatctgaa gagcttttaa tcccccgatg gttcttcacc
    10441 tttgcagcaa cgagtattgc tctggatgaa tcaattatat cctgggtgta tttccatcga
    10501 catgagcaga ctcggctgtt gaggatttcg cctgctgccg aaaaaaaact tgcgacagtt
    10561 tcagaggatg tggtatgcta tcaggtgttc acctgaggat cgcggttgaa aacatggatc
    10621 ggagtcccga tacagggcaa atcagaggag gttcatgata tgaagcgcat tgggcagaga
    10681 ggttactgtg cggcggtggt atacgtattg gttctggcgg cgcccgttct ggcggcccca
    10741 acacaattgc tgcaaaacac cagtttcgag agtccgatca gcggctcgcc gccggattac
    10801 tgg
//
LOCUS       DAOTPW010000196        10776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5743_length_10776_cov_4.871840, whole genome shotgun sequence.
ACCESSION   DAOTPW010000196 DAOTPW010000000
VERSION     DAOTPW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10776
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5743_length_10776_cov_4.871840"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="P5316_16005"
     CDS             <1..1775
                     /locus_tag="P5316_16005"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRR86514.1"
                     /translation="MKNRNDGLAAWLYRRTTPVPGTPRILLWDDPGLREKEPQAGACI
                     RAFQDIGQVVMRCGWGPYDPMAAIKFHQNRGHHDIGTFYLFGGGRPTIIDSGSANYNS
                     PIYGSCLSRTVAHNVVMVDNENQVRADGELLAAVGTSLIGAASGQLKAAYPDKLESWT
                     RDLVFTRHGLVVICDRLEGKGEHQFDLILHPENPFGMSGPNDLEIGRGPHKCLLQVLA
                     DMPLQAILQDGYHRTLPRKYVRFRAEKPASHVSFVTVCRWPTQVGSGFVVPHCVVRSL
                     GPGSYQTVDEEGEESQAFWLSVTGADSGNKERLRSDARVAAAMPTGIRPSGREEVQDG
                     AVVFLNGTYVNLRGAEVLRANHRLSGAVEWPHLKDAPQMIAQFWAERPTSVTLAVDRG
                     AQVKWVRLGGRPVDFKQHENLVSIALPEGRSELTIGSYPRLSPARTGIEDARYGTDLL
                     HVRADVNVPAYQEGVRTRASSSWTDGVDALDGDVNTSWVSLPGLPMPQWLEVRLPQPT
                     GMTEMEIRTPLPTAGRVESWDMGANEWRLLGPFETTADQPAATVVFSRILTDRFRAVV
                     ERIDPANQSAIIAEWRWSAAVSRR"
     gene            complement(1807..2655)
                     /locus_tag="P5316_16010"
     CDS             complement(1807..2655)
                     /locus_tag="P5316_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR86515.1"
                     /translation="MKMIKCISYWSTKDGLAGKTTIEEALRVTREAGFAGLELCIGTD
                     GVLTTSTSQADCEAIRKKIDASGVIVETLASGMCWGFNPTSDDPAVRQKSIELHSDAL
                     QRAAWLGCRAMLYVPGVVKSPICPDVVRYDIAVERAREGVKKLLDVAEKVGVDLCVEN
                     VWNGMFYSPLEFIQFIDSFGSKRLGVYFDVGNGMGYHQHPPHWIELLGKRIKRVHIKD
                     YNLNFGWLGAYSFCDLGAGQVPWAEVMDALRGVGYNATLVAEMMPWDPGTLARTSAAL
                     DVIMRM"
     gene            complement(2762..3388)
                     /locus_tag="P5316_16015"
     CDS             complement(2762..3388)
                     /locus_tag="P5316_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86516.1"
                     /translation="MAQNEQVLVVERRVVEEVGMFHGLQFDVERYLDKLFAKDVPRFM
                     LRSQAEKDPAYKQLIPYVIMTHDGKYLSYVRGRRAGEARLIGLRSIGIGGHINPGDDM
                     PLFSSDFRDAYRTAVEREVAEEVSVEAGHTDHIVALLNDDRNEVGQVHLGIVHLWVLD
                     APKVTKREQMITQMSFMTPAELHEIRDDMETWSSLCADGLEEMVRRTR"
     gene            complement(3674..4699)
                     /locus_tag="P5316_16020"
     CDS             complement(3674..4699)
                     /locus_tag="P5316_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR86517.1"
                     /translation="MITSTCHRSGSGKVIEAGSSNRRQTAFTLIEVLVVVAIIALLIA
                     ILLPSLKAARENARATVCGHQQRQLAQAGTIWMTEGNKNMVPAHRGWATHVLRVMKGQ
                     ADMFKCPSTETLVPIAPVLIRQYRTGFTYPALSTDSPYFRRNPNPDGQGFYRADMETE
                     AMEQLGSGAGDADFDDAYVYTKPLGDGKIAEVYAVKAGTGRALSMFDYKGKLLAEYFS
                     TTPRYQLPTLWGSYGMNLSAAIPGAKPYNILYLDYTDWAAVVESRFGVISAYDGKRRG
                     DGKPGREKYEWVDPRHNRRVNVCFLDTHVERMVPQKLVPPLNEDLPSPWHPQRPPGWI
                     VPGLPAE"
     gene            complement(5051..7666)
                     /locus_tag="P5316_16025"
     CDS             complement(5051..7666)
                     /locus_tag="P5316_16025"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HRR86518.1"
                     /translation="MKRNMIKSTFWTVGVMVAALGVFGQNLLAGTPYPGPASHPIPGV
                     IEAENYDADGANVSYFDTTAGNSGSTYRADDVDIQIASGGSDVEGTVVYNVASTEWME
                     YTVEVASAGTYKLVMRCAVNTSGTQVQFKLDGNAITPVTTLLSGGAWNRWDEMAWTVS
                     LPAGQHVLRFEMVAGSASEGVKINWFSFTPYVETFDRPGLAFWRFDNDGGGPTVLDQT
                     ANGNNGTATNVAYSALIPPVAWPTIPYPGATTPTPPAAPNDAPAPNWPAHGAPRNATS
                     ADFERDMGAYITVADSETLDRGADKSWTVEAWVKLETAGDIGNSTTRQWLIHKKAFMT
                     SDNFTDYAVLVNGADYVNNATWINPEPDWPNYAGLTGKEIVVLFGRADGSGTDRVVSR
                     LQVYDPAASGGALWHHVTVSYNAVKKEVIFVVDNLPPERVTGLSPTNVPNNGPLVIGG
                     HIMGNANSVNQTFDGQIDELWIAPEPLAGTPGNTGAPLFVPWFWENEGVAMDGTFTTE
                     DKAPWKERPSKMSEDNSCFWQVETDPADSANAILHLFDDANLAAYYFWDHDTFNKVDS
                     AAYKKETCTPQNMSQGLAWTCRFKLTRHMRNELGSPTQSQKFLRFVSDLQGATGANAH
                     FGITVQHTNETTRTGISVRDDAGGTGWVSGDITNEWHVLHGAAWVLADNEVQHKLWLD
                     GTEVASWVRTSRQTTFDPYIGFNDNNRRFMSEGYIDYVKMTGAGGAWAPDGTRLGTWL
                     EICDNGVDDDGDTLADCADPDCLGWRAEVCTNGIDDDCDGYTDCADSDCVNNPVCCTH
                     DPAFDIDDDGDVDQADFAVFQACFMTDWATMSADCRCMESDGDNDIDQTEYDAFEACA
                     SGAGISANPACDDPL"
     gene            8503..9363
                     /locus_tag="P5316_16030"
     CDS             8503..9363
                     /locus_tag="P5316_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR86519.1"
                     /translation="MGRRSLSVSGDSRGRRADLGFTLIEVLVVLAIIGLLVAILLPSL
                     SKARAIARATVCLSNLKQFGSAEMAYGAENNGYMARGGDANAQHWIKLLPRQFGDRRI
                     YKNVNEVPVEEFEIFRCPERSLTSPTPFVDYVVNAVRAEIGPGMWDRPYAEIEAQGPT
                     PLNDWKCPARVIVIGDAALEKGKGDQPDATTADHLTEARVKHGEVMRKLRPASEGKLD
                     LLDVFKPRYMQRSSERRAGTRIHLKSFCNWLHGDGHAERILWLSDKRTGDQWMRMYGV
                     RDPKVTSDVP"
     gene            complement(9500..10264)
                     /locus_tag="P5316_16035"
     CDS             complement(9500..10264)
                     /locus_tag="P5316_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86520.1"
                     /translation="MINAEYAYYLRDMDGDGIVDKPNSATLEQIRHATWDIAMASGYF
                     VTAWGTTYWNGFRAPGPFNPDDPRNDDWEEDVQHIRELFTSVPWWELEPADARVSGPG
                     IHYCLANGLDAHLVYVRQAGGPISLSLQGAPRVLYAVERFDPRTGAYLSLPHHEAPIA
                     LSISPPDSQDWVYLVTAVPGSLVATGDFDRDNDVDQADFGRLQGCLTQSPHVPSPGCE
                     IADLDLNGRIDQADVNVLRACMNGPQIDPPSDCLQD"
     gene            complement(10343..>10776)
                     /locus_tag="P5316_16040"
     CDS             complement(10343..>10776)
                     /locus_tag="P5316_16040"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HRR86521.1"
                     /translation="AELMNHGGWSANTGGEPFTSYTDETINPAYWQEVDGRVGDLNAA
                     GITASLFLAWNNSDPAPPSWKSFASDAARLRCARYIVARYSAFNVLFVVVGEWQESGP
                     YAPLYGAVGSQMAADDPHDRPIAIHCTPDPGTVEPQFATAP"
BASE COUNT         2048 a         3224 c         3274 g         2230 t
ORIGIN      
        1 tgatgaagaa tcgcaacgac ggcctggccg cgtggctata tcgccgtacg acgccggtac
       61 ccggcacacc gcgcatcctg ctatgggacg accccggact gcgcgaaaag gaaccgcagg
      121 ccggcgcatg tatccgagcc tttcaggata tcgggcaggt ggtgatgcgt tgtggctggg
      181 gcccgtatga ccccatggcg gcgatcaagt ttcaccagaa tcgcggacat catgacatcg
      241 gcacgtttta tctgttcggc ggcggtcggc cgacaatcat cgactcgggc tcagccaatt
      301 acaacagtcc catttacggc agttgccttt ccaggaccgt cgctcacaac gtggtcatgg
      361 tggataatga gaatcaggta cgcgcggacg gcgagctcct tgcggccgtc gggacttcgc
      421 tcatcggtgc ggcatcggga cagctcaagg ctgcatatcc ggacaagctg gaatcgtgga
      481 cgcgggacct ggttttcaca cggcatggcc tcgtcgtgat ttgtgaccgg cttgagggca
      541 aaggcgaaca tcaattcgac ctgattctgc atcccgagaa tccctttggg atgagcggtc
      601 cgaacgatct ggaaatcggt cgcgggccac acaagtgcct cttgcaggtg ctggcggata
      661 tgccgcttca ggcgattctc caggatggct accatcggac cttgccgagg aaatacgtcc
      721 gcttccgcgc agagaagccg gcttctcacg tgtcttttgt cacggtgtgt cgctggccga
      781 cacaggtcgg ctccgggttt gtggtcccgc actgtgtcgt gcgttcgctt ggacccggct
      841 cgtatcaaac cgtggatgag gagggcgaag aatcgcaggc tttctggtta agcgtcacgg
      901 gcgcggactc gggcaacaag gagcggctcc ggtcggatgc tcgtgtagct gctgccatgc
      961 ccacaggcat caggccatcc ggccgagagg aggttcagga tggggccgtc gttttcctca
     1021 acgggacata cgtcaatctg cgaggcgcgg aggtgctcag ggccaaccat cgtctttccg
     1081 gggctgtcga gtggccgcac ctgaaagacg ccccccagat gatcgcccaa ttctgggctg
     1141 aacggccgac gtcggtcacc ctcgctgtgg atcgtggagc gcaggtgaag tgggtccggc
     1201 ttggtggccg gccggtggat ttcaagcagc atgagaacct ggtgtcgatc gctcttccgg
     1261 aagggcggag tgaattgacc atcggttcat acccgcggct gtcccctgcc cggacaggta
     1321 tcgaagatgc gcggtatgga accgacttgc ttcacgtcag ggcggatgtc aacgtaccgg
     1381 cctatcagga aggcgtccgc acgcgggcca gtagttcgtg gacggacggg gttgatgctc
     1441 tcgacggcga tgtcaacacg agctgggtgt cgttgccggg gctgcccatg ccgcagtggt
     1501 tggaggtgcg tttgccgcag ccgacgggga tgacggagat ggagattcgc acacccttgc
     1561 cgacggccgg acgcgtggaa agctgggata tgggggctaa tgaatggaga ttgcttggcc
     1621 ccttcgagac caccgccgat caaccggcgg caacggttgt cttcagccgg atcctgactg
     1681 atcgtttccg agcggtcgtg gaacgtatcg atccggccaa ccagtcggca atcattgccg
     1741 aatggcggtg gtcagcggcg gtgtctcgga gatgattcat cgacggcccg aaacctcgcg
     1801 gccggcttac attctcatga tcacatcaag agccgcgctg gtgcgggcca gggtgcccgg
     1861 atcccagggc atcatttcgg ccacgagggt tgcgttgtag cccacgccgc gaagggcatc
     1921 catcacttcg gcccagggaa cctgcccggc acctaggtcg cagaatgagt acgcgccgag
     1981 ccaaccgaag ttcagattgt agtctttgat gtgcacgcgc ttgatccgct tgccgagcag
     2041 ctcgatccag tgcggcggat gctgatggta acccatgccg ttgccgacgt cgaaataaac
     2101 ccccagacgc ttgctaccga atgagtcgat gaactggatg aactccagcg gcgagtagaa
     2161 catgccgttc cagacgtttt cgacgcacag atcgacgccg actttctcgg ccacgtccag
     2221 cagcttcttg acaccctcgc gggcccgctc gacggcgatg tcgtaacgca cgacgtccgg
     2281 gcagatgggg ctcttgacca cgccgggcac gtagagcatg gccctgcaac ccagccaagc
     2341 cgcccgctgc agagcgtcgg agtggagctc gatcgatttc tgccgcacgg ccgggtcatc
     2401 gctcgtcgga ttgaagcccc agcacatccc gctggcgagc gtctcgacga tcacccccga
     2461 agcgtcgatc ttcttgcgga tggcctcgca atcggcctgc gaggtcgaag tcgtcagcac
     2521 gccgtcggtg ccgatgcaca actccaaccc cgcaaacccg gcctccctgg tcaccctgag
     2581 cgcctcctcg atcgtcgtct tgccggccaa gccgtctttc gtggaccagt agctgatgca
     2641 tttgatcatt ttcatgccct cacttcgtgg tggagttgtt gctgcctttt tccggcgaca
     2701 ccctatcgcc atggcttcga ggcgtcaaca gaatacgccg tatcgggtca accgccgtgg
     2761 ctcatcgcgt tcgtctgacc atttcctcca agccgtcggc gcacaggctg gaccaggttt
     2821 ccatgtcgtc gcggatctca tgcagctcgg ccggcgtcat gaaggacatc tgagtgatca
     2881 tctgctcgcg tttggtgacc ttgggcgcgt ctaggaccca taggtggacg atcccgaggt
     2941 ggacctggcc gacttcgttg cggtcgtcgt tgagcagggc cacgatatgg tcggtgtggc
     3001 cggcctcgac ggatacttcc tcggccacct cacgttccac ggccgtccgg taagcgtcgc
     3061 ggaaatcgga gctgaacagg ggcatgtcgt cgccggggtt gatgtggccg ccaatcccga
     3121 tggaccgcag cccgatcagc cgcgcctcgc ccgccctgcg cccgcggacg tagctcaggt
     3181 acttgccgtc gtgagtcatg atgacatacg ggatgagctg cttatacgcg gggtccttct
     3241 cggcctgtga ccgaagcatg aaccgcggaa cgtcctttgc aaaaagcttg tcgagataac
     3301 gttccacgtc aaactgcagg ccgtggaaca tgcccacctc ttccaccacc ctccgctcga
     3361 ccaccagcac ctgttcattc tgggccatga agtcactcca ggcgcgcggt tgcgggtgcc
     3421 acggccacag ctctttcaga tgagccgttt tggccgtagg ccccgagcgc ctggcggaat
     3481 ggtaatggat tcgcccagca tgcaaaagcg ctccgggcag atcatgccaa ggggctacgg
     3541 ggtgtcgtga ggtggtgtcc ttacaagtga agccggtcac gttggacctt gacttgagac
     3601 aagaacgctt ccgcagcagc agggatttgt taaatccccc agatgcaggc ccttctcgta
     3661 ctcgtgactt ctgtcactcc gctggcaggc cggggactat ccagccgggc gggcgttgcg
     3721 ggtgccacgg cgagggtaaa tcctcgttta ggggcggaac cagtttctgg ggtaccatac
     3781 gttcgacatg cgtgtccaga aaacagacgt tcactcgcct gttgtggcga gggtcgaccc
     3841 attcgtactt ctctcttccc ggcttgccgt cgccccggcg tttgccgtcg taggctgaaa
     3901 tgacgccaaa ccggctttcg accaccgccg cccaatcggt gtagtcgagg tacaggatgt
     3961 tgtacggttt cgcgcccggg atggcggccg acaggttcat tccgtagctg ccccacaggg
     4021 tgggcaactg gtaacgcggc gtcgtgctga agtactcggc aagcaacttg cccttgtaat
     4081 caaacatact cagcgcgcgg cccgtccccg ccttcacggc gtagacttcg gcgatcttgc
     4141 cgtcgcccaa gggcttggtg taaacatagg catcgtcgaa atccgcatcg cctgcaccgc
     4201 tgcccaactg ctccatcgcc tccgtttcca tgtccgcccg atagaagcct tggccgtccg
     4261 gattcggatt tcggcggaaa tatgggctgt ctgtcgagag ggccggatag gtaaagcctg
     4321 tccggtattg tctgatcagg accggggcga tgggcaccag tgtttccgtg gacgggcatt
     4381 tgaacatgtc ggcctgccct ttcataaccc tcaggacgtg agttgcccat ccccggtgcg
     4441 ccgggaccat gttcttgttg ccctcggtca tccaaatggt gccggcctga gccaactgcc
     4501 tttgttggtg tccgcagacg gtagccctgg cgttttccct cgccgccttg agcgagggca
     4561 agaggatggc aatcaacagt gcgatgatcg ccacgacgac taggacttcg atcaacgtga
     4621 aggccgtctg ccggcggttg ctcgaaccag cctcaataac ctttccgctc cctgaacgat
     4681 gacatgtgga agtgatcatg ttgcacctcc tggcatccct gagcaaccaa gcccatccct
     4741 gaacatagtc ttctatgatg accgtttact attatacctc atagacgtcc gtcttggcca
     4801 aacaaaccct cgcggcccca tgcctgcggc taaagctttt ccgctacgcg gctcggcgtc
     4861 ggccggcaag tcgggtcgat cggacacacc ggcgttcgca agaccacggg ccgtggccgc
     4921 cgcgatgccc ttatcgtggg gccgccttaa agcttcgaaa ccaattgctg atcctggcat
     4981 tagtacgcat tgccagtaca cgaacgctcg cgtgccggaa tcatcgccgc tgcgcggtgg
     5041 cgccccccga tcataatgga tcgtcacacg ccgggttggc cgatatgccg gctcccgagg
     5101 cacaggcctc gaaggcgtcg tactctgtct gatcgatatc gttgtcgccg tcggattcca
     5161 tgcagcggca gtcggcgctc atcgttgccc agtcggtcat gaagcaggcc tggaagacgg
     5221 caaaatccgc ctggtccacg tcgccgtcat cgtcgatgtc gaatgccggg tcgtgcgtgc
     5281 agcagaccgg attgttcaca caatccgagt cggcgcagtc ggtgtaaccg tcacagtcgt
     5341 cgtcgatgcc gtttgtgcag acttcggccc tccagccaag gcagtccgga tcggcacagt
     5401 cggccagggt gtcaccgtca tcatccacac cgttatcgca gatctccagc cacgtgccga
     5461 gcctcgtgcc gtccggagcc cacgcgccgc ccgcgccggt catctttacg tagtcgatgt
     5521 atccctcgct catgaaccgg cggttgttgt cgttgaagcc gatgtacggg tcgaaggtcg
     5581 tctggcggct cgtgcgcacc cagctcgcga cctccgtgcc gtccagccaa agcttgtgct
     5641 gcacctcgtt gtcagcgagc acccacgccg ccccgtgcag cacatgccat tcgttggtga
     5701 tatcgcccga aacccatccc gtacctccgg catcgtctct gaccgaaatg ccggtccgtg
     5761 tcgtctcgtt cgtatgctgc acggtaatac cgaaatgagc gttcgcgccc gtcgccccct
     5821 gcagatcgct gacaaaacgc aggaacttct gcgactgggt cggtgaaccc agctcgttcc
     5881 gcatgtgccg cgttaacttg aaccggcacg tccaggcgag gccttgcgac atgttctgcg
     5941 gcgtgcaggt ttctttcttg taggccgccg aatcgacctt gttgaaggtg tcatgatccc
     6001 agaagtaata agcggcgaga ttggcgtcgt cgaacaggtg gaggatcgcg ttggctgaat
     6061 ccgccggatc cgtctcgact tgccagaagc aagaattatc ctcgctcatt ttgctgggac
     6121 gctctttcca cggagccttg tcctcagtgg taaacgtgcc gtccatggcc acgccttcgt
     6181 tctcccagaa ccacggcaca aagagcggcg cgccggtgtt gccgggagtc ccggccagcg
     6241 gttccggcgc gatccacagt tcgtcgatct ggccgtcaaa ggtctggttg accgagttgg
     6301 cgttgcccat gatgtggccg ccaatcacca gcggtccgtt attgggtacg ttggtcggag
     6361 acaagccggt cacccgctcc ggcggcagat tatctacaac aaagatgact tctttcttaa
     6421 ccgcgttgta tgacaccgtt acatgatgcc acaacgcacc gcccgaggcg gccggatcgt
     6481 agacctgcag tcggcttacc accctgtcgg ttcccgatcc gtcagctctg ccaaacagga
     6541 ccacgatttc ctttccggtc aggcccgcat aattcggcca atcgggctcc ggattgatcc
     6601 atgtcgcgtt gttcacatag tcggcaccgt tgaccagcac cgcgtagtcc gtgaagttgt
     6661 cgctggtcat gaaggctttc ttatgaatca gccattggcg cgtcgtgctg ttcccgatgt
     6721 cgccggccgt ctcgagcttg acccaggcct cgaccgtcca gctcttgtcg gcgcccctgt
     6781 ccagagtctc gctatctgcg acggtaatgt aagctcccat atcccgctcg aaatccgccg
     6841 aggtggcatt gcggggggcg ccgtgcgccg gccagtttgg agcgggagcg tcgttcggtg
     6901 ccgcgggagg cgtcggagtg gtggctcccg ggtatggaat cgtcggccaa gcgactggcg
     6961 gaatcaaggc tgaataagca acgttggtgg cggtgccatt gttgccattg gccgtctggt
     7021 caagaacggt cggcccgccg ccgtcgttgt caaaccgcca gaaagccaga ccgggccggt
     7081 cgaaggtctc aacatatggg gtgaacgaga accagttgat cttgacgcct tcagatgcgc
     7141 tgcccgcgac catttcgaat cgcagcacat gctgtccggc cggtaagctc acggtccagg
     7201 ccatttcgtc ccagcggttc caggctccac cgctgaggag agtcgtcacc ggtgtgatcg
     7261 cgttgccgtc gagcttgaac tgcacctgcg tgccgctggt gttcaccgca cagcgcatga
     7321 ccagtttgta agtaccggca ctggccacct cgactgtgta ctccatccac tcggtcgacg
     7381 cgacgttata gacgacggta ccttccacgt cgctaccgcc ggaggctatc tggatgtcca
     7441 cgtcgtcggc gcgatacgtg cttcctgagt tgcccgccgt ggtgtcgaag taggacacgt
     7501 tggcgccgtc tgcgtcgtag ttctccgcct cgatcacacc cgggatggga tgcgatgccg
     7561 ggccgggata cggggttcct gcaagcagat tctggccaaa gacaccgagg gcagccacca
     7621 tgacccctac ggtccagaac gtacttttga tcatgtttcg tttcacgatt ccttcctcct
     7681 cacaagagac tctgcgtgcc tgaatggaga gttgcgcgcg ttgcatatac gacgcttaat
     7741 cgattcaccc gagagccgca tccgcctctc cttcccggtc gaaacgcctt caaaaaacat
     7801 catccaacaa ccacatccct gaactgctga aaatcgctgt cagttccctg tggtgctatc
     7861 ggcactccag ggaaacaacc acaagttaac agaatccttt cagtaaatca agcgggttta
     7921 gcagccgagg agccaaaaaa acgccaagga tcgccggtca cttgaagata agtagccatg
     7981 tattagcacg acctatactg aaattttagc ttgtcctggc gaagtgggtg aaatggttcc
     8041 gaccgaggtg tttttgaaat gccagattgg ccaggtgggt gcggcgatcg caggtttcgg
     8101 tcgcctgatc atcttagcta agacaacaaa acgcatcgga acagacgtaa gtatttttat
     8161 atcaaccctt tgtgtcgcca aggaggagag gcaagcggct cttttcgaac atggcgcatg
     8221 gacagcccgg cggtgaaatt ggttggcgtt gctcgctcaa gggttgtgaa ttcctgtgca
     8281 gatattggct gatgcgccga tgcagacaag ccgattcgga ggacattatt ggcggagcat
     8341 cgttccgggc tgaagatctg gtgagatgga aatctgaaga gagttgcatt tcgggcttga
     8401 atcgtttaag atgctcatgg tgtcatcttt cgaaggtcaa caccgatggt ttagctatgt
     8461 ttttttttcg cgcgacgggc cgtatttgta ggaggtgcag gaatgggacg tcgctcgctt
     8521 tcagtttcag gggattcaag agggcgcagg gcggaccttg gtttcacctt gatcgaagtg
     8581 ctggtcgtgc tggccattat cggcctgctg gtggcgattc tgctgccatc gttaagcaaa
     8641 gcccgggcta ttgctcgcgc gactgtatgc ctcagtaatc tcaagcagtt tggcagtgcc
     8701 gagatggcat atggtgctga aaacaacggt tacatggccc gcggcggcga cgcaaatgcc
     8761 cagcactgga tcaagctact tccgcggcag ttcggggatc gccgcatcta caagaacgtc
     8821 aacgaggtgc cagtggagga attcgagatc ttccgttgcc ctgagcggtc gctgacgtcg
     8881 ccgacgccgt tcgtcgacta tgtggtcaat gccgtgaggg ccgagatcgg tccgggcatg
     8941 tgggaccggc cgtacgcaga aatcgaggct caaggcccta cgccgctgaa cgattggaaa
     9001 tgcccggccc gtgtgattgt catcggcgac gcggcccttg agaagggaaa aggcgatcag
     9061 ccggacgcga ccacggctga tcacttgacc gaagcgcgag tcaagcacgg tgaggtcatg
     9121 cgcaagcttc ggccggcttc ggaaggcaag ttggatctgc tcgacgtctt caaaccgagg
     9181 tacatgcagc gaagctctga acgccgcgcc ggtacgcgaa tccacctcaa gagcttctgc
     9241 aactggttgc acggcgacgg ccacgccgaa cggatccttt ggctcagcga caagcgaacc
     9301 ggtgaccaat ggatgagaat gtacggcgtg agagatccaa aggtgacttc cgacgtcccg
     9361 tgatctgcga acgggcgggt gcgagcgagc gaagttgtgc gatgccgccg cttcctccgc
     9421 gggcggcatt cccattatgg ctggagccgc gggccgtctt tgcacaagcg agtgaaccag
     9481 tcgggggaga tgaggcatct cagtcctgca agcaatccga gggaggatcg atctgcgggc
     9541 cgttcatgca ggcccgcagc acgtttacat ccgcctggtc gatacgccca ttgaggtcga
     9601 gatcggcgat ttcgcaaccc ggggagggga cgtgagggga ctgcgtgaga cagccctgaa
     9661 ggcgaccaaa atcggcctga tccacatcgt tgtcccggtc gaagtcaccg gtggccacca
     9721 gcgaaccggg caccgccgtg accaggtaga cccagtcctg gctgtccgga ggactgatcg
     9781 acaacgcaat cggggcctcg tggtggggca gtgataggta agcacctgtt cgcgggtcaa
     9841 atcgctcgac cgcgtacagc acgcgaggag ccccctgcag cgagaggctg atcgggccgc
     9901 cggcttgcct cacgtacacg aggtgcgcgt caaggccgtt ggccagacag tagtgaatgc
     9961 ctggcccgct cacacgggcg tcagccggtt ccaactccca ccagggcaca gacgtgaaca
    10021 actcacgaat gtgctgaacg tcctcctccc agtcatcgtt gcgcggatcg tcgggattga
    10081 aaggaccggg ggcgcggaaa ccattccagt aagtcgtgcc ccatgcggtg acaaagtagc
    10141 cgctggccat cgcgatgtcc caggtcgcat gtcggatctg ttcaagcgtc gcgctgttag
    10201 gcttgtccac gatgccgtcg ccgtccatgt cccggaggta gtaagcatat tctgcattga
    10261 tcacgggctt atcgtacacg cgagcgctga ggatcccctg atgaagatcc gtgtatctct
    10321 gctggaagtc gccgaatgac atctacggtg ccgttgcgaa ctgcggctca acggttcccg
    10381 gatcgggtgt gcagtgaatg gcgatcggcc gatcatgcgg gtcgtcggct gccatctggc
    10441 tgccaacggc cccataaagc ggggcataag ggcctgactc ttgccactca ccgaccacga
    10501 caaaaaggac gttgaaagcg ctgtaacgtg cgacgatata tcgcgcgcag cgaagccggg
    10561 cggcgtcgga ggcaaaggat ttccacgagg gcggtgccgg atcgctgttg ttccatgcca
    10621 ggaagaggct ggcggtaatc ccggcggcat tcagatcgcc gacccgcccg tccacttcct
    10681 gccagtaagc gggattgatg gtctcgtccg tgtagctggt gaacggctcg cctccggtgt
    10741 tcgccgacca gccaccgtga ttcatcaact cggcgt
//
LOCUS       DAOTPW010000197        10768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5749_length_10768_cov_5.332120, whole genome shotgun sequence.
ACCESSION   DAOTPW010000197 DAOTPW010000000
VERSION     DAOTPW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_10768_cov_5.332120"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..918
                     /locus_tag="P5316_16045"
     CDS             97..918
                     /locus_tag="P5316_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86522.1"
                     /translation="MRDTYRYSLLLTQRGQRYTLALAALALAAGCSNSSRDFRPEDQF
                     GRIYYIDGAGNWGFGQLAVPQGLREAGYRGRIATYRWSATLNPLLDQTVFRAVARAQG
                     RKLAREIEHYLAEYPNNEVHLIALSAGTGVAVWACEHLTPPAKIGNVILLSSSLSSEY
                     EMSKALANVRGTIFVYYSPNDGVLGGPVQVLGTIDGALGSHPAGLVGLRGLSSKIRNI
                     PWTRRYEAYGWTGTHVSAVHHDFVRHVLSQHVLPEAQMLTAEVAHLSGTGEASQQ"
     gene            complement(944..2257)
                     /locus_tag="P5316_16050"
     CDS             complement(944..2257)
                     /locus_tag="P5316_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86523.1"
                     /translation="MNNAACGSRMRRWGWLIVLFVIAAGLRAGWVCFRYGGPASPGHE
                     YPDEKAYQDVARSLAAGDGLVDEFGYRATYMPGYPAFLALFADGSDAFSPGRSYWARL
                     TQAILGAFIAPVVYILAAGWLSLAGFVSEQAGSRTAVPVLAGLAVAIDPFLVFFSGLL
                     LTETLFVVALALAWTCVLSLCRRDRRVSPAPVIGAGLMLWLCLMLRPSAVVLVPPVAV
                     AIVLARGFDRQGLAAAVSVLLLVAVGLSPWAARNRRVIGEWRWLTTRGGISLYDGFQS
                     QATGASDLAHTKTMPQVQGLSEVEWDRFFRNQAWSAIRQDPGRAVRLAWAKFRRTWSP
                     WPNVENYRGSAAGLAGALWTICILLSAFPGWWGYRRAVGAWMVLLLPVAAFTLLHMVY
                     VGSVRYRLPVMPFVTILAAAGLVRLVAVLRGGCPVGGRTPSSPLR"
     gene            complement(2282..3757)
                     /gene="tilS"
                     /locus_tag="P5316_16055"
     CDS             complement(2282..3757)
                     /gene="tilS"
                     /locus_tag="P5316_16055"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRR86524.1"
                     /translation="MAVEDLESRFAQDAERSGLVPRGAAVVAGVSGGADSVAMLHLLC
                     GLNRRGWGLRIHVAHLNHQLRGAEADKDARFVRELAEGLLLDCTVGTADVRARASQDG
                     VSIEQAARRCRFEFFERVCLKSGAGTVVLAHHADDNAETILHRIVRGTGLRGLAGIRG
                     SRPIRPGSGIQVVRPMLSFRRSEIEEYVRDRALPFRHDATNDADSYTRNRIRNDILPL
                     LRDSINPQVAEALVRLGEQARDVDEYLTETAGRMLESMIVERDDRQIVLLSTSLARKP
                     RLIKTQLIREAMLRLGVSEAEIGYSHLTAVAAMAEETEGSKAMDLPGGLRVFRRYDRL
                     VLQLEAEPAGVAPVPGEIRVGIDGRTSLLPFGIELSVETLLADQSLIAEHIRTAASRG
                     QVSWEEWIDADQVHPPLIARLRRPGDRFLPLGMSELKKLSDFFIDEKIDADVRERTVL
                     LCDRLGPIWVVPLRIDDRVRLTEATRRVMRLSARPLSAPRD"
     gene            4159..6045
                     /locus_tag="P5316_16060"
     CDS             4159..6045
                     /locus_tag="P5316_16060"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HRR86525.1"
                     /translation="MLSNAVLLAIGSLGYLAGPFTVYDLRYALRFNAQNPQEVAAAWD
                     HVHAVATLQGIVNREEANLYILFVESGGRCIDEYWLGRMSEPGQWLAGRTRAMVPDLG
                     ALIDKYKKLVKGAVVYDPAVPATSNLASTIAGVENLIAVRYDPSPNSLYSRLITSGPR
                     LPVVRRLLNENGSSMFTGQGNIPGTDQPSTGSAKCDAYLWLKHHYIDTGKVEAGYAGY
                     YIDSFWQKCPTAAGPNQHTLSNHDFFVAKKAFFFDLNVWADETPIDDRAQKPGTDLQT
                     FKALLRSAYDRTSGRQMLHIGGFTPWAFKYTNAGKAGGTHEPVPTEWETARLIGAYNA
                     FIDADALGSAAMANASFYAHFPLNKRYPQPWVTKQRLINRGLLQPNGQVNFDGRQFVI
                     FYVGDFDSAAWLYQFVPVVWDHPGRGKLPLMWSISPVLERRAGMAMDYLRRTATPNDY
                     FAAADNGAGYLNPGMLQEPRDISGLPSGLDLWAEHCTPIYARWGITITGFIIDGYAPG
                     LNKDGLDCYAKFSPNGIVPQKIPATLLHGNMPVLRAGYDLGDNAERAARTIVGRVARR
                     SVPFHWFRGILKSPDWYIEVYDRARAANPKIELLDAPAFFELYRAWLKSNPQAAAGKI
                     DCER"
     gene            complement(6215..7504)
                     /locus_tag="P5316_16065"
     CDS             complement(6215..7504)
                     /locus_tag="P5316_16065"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86526.1"
                     /translation="MIDSRLTRRSFIRQGLVGGTVAGAASLGLNAASIARVIGANERI
                     NLGLIGGGHRGRFIVEQMAKGSGDNFALVAFGDIWTQRRESYPAEAEKILGIKPKAYA
                     DYRKLLEDADVDAVVIATPDHQHAGQAIDAVQAGKHVYVEKPIIGLHVDLHELNRLYD
                     VVKASKMVVQHGTHGVSSPAVRAIKELIAEGKLGKLFRVESTETAYVPYWAHYQGPAT
                     VSETDWKAWLYNRPERPFDANMCAKWMGYKEITSGTIGGWMTHFITFVHFVTGCDYPL
                     SAVAWGGRYAPTNDPKCTAPDQTMVMLDYAEGFHTQFTSHFGSEIDNETTVFMFEKGC
                     VKTRFGHHPGNPLVSGEGTNNKLEAHKLLGETVDPPYPGAAHVKNWFDCIRNNGRPNA
                     DMDLGHKQGVAVALGDLAWDLNCKVVYDKAKREVRRA"
     gene            complement(7644..8756)
                     /locus_tag="P5316_16070"
     CDS             complement(7644..8756)
                     /locus_tag="P5316_16070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86527.1"
                     /translation="MMMTRREFVSGAVAMVSGMTTSMAQSARQAATDRAFKALRVAQV
                     GVQGHFGDIVTGIPKVEGCSLVAVARSFPDEPVEQLKEKPVWNPDTRIFDDYRQMLDE
                     VRPDIVAAFAPYAHNGQVSIEAVRRGCHVISEKPLASTLEDLDALRAERDRAKVRVTA
                     MLAMRFHPGLMAAHKAVQEGSIGEPLLISAQKSYAWGQGRPWYFKLRKNYGGSIPWVA
                     IHAIDFIRFVTGLDFTSVTARQAVKVHKDYPECEDCGALLFDMSNGGQATLTFDYFRP
                     PKAGSHGDDRLRVAGSRGVVEVRITNTTFCELITDEQGPAQLPLSDESHNIFIDFVES
                     LRGLRPHFLSEEDPFRATEVALKARDSADRRVTVAL"
     gene            8846..10159
                     /locus_tag="P5316_16075"
     CDS             8846..10159
                     /locus_tag="P5316_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86528.1"
                     /translation="MGCTVAMLLVLAANPGTPSSSPAASRPTTTPATVRIEYYRLRTA
                     DGQHDVQQCSALHFGRLGRRDGLWMTSDRNGGPSAGKIHFISAATLADAQHQGTIQVD
                     ECFTVVPPAEGWETFASSHSEIPAEILDDLHDRVEARLTGDKGPYLDLEAVTIAPSIL
                     PSHETSLFVAAEEPYSTVLELVPLAHGPDAQAQILSLHIYEEAEDERGSDRNDGLEAL
                     AWAGQPGFFYWAEEGTSYHGGDPGPRLFFRDPKVGRGRLIGGRLAIQQDLTHKLTSAV
                     WTLREGPEQTLNALTVLPSGRLLAVDRNGAWILRLDPRAGTAERWLNLHDINGLDLRK
                     LLHDFPAPRRMPYISIEGISVDPAGDIWLIDDPALPEAFRASCLIRLRGLNPDAAGPS
                     QEKRWRADTDQSSADRRKLIADGLYYQSATDPSSCRSSGVVQWPS"
     gene            complement(10096..>10768)
                     /locus_tag="P5316_16080"
     CDS             complement(10096..>10768)
                     /locus_tag="P5316_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRR86529.1"
                     /translation="WATKIVDSPAVVERAVDLMAAVREEAGSGFDLALDCHGRLSPAM
                     AIRFAKALEPYHPMFLEEPCLPENVDTMVTVARSTSIPIATGERLFTRWGFREVLEKQ
                     AAAIIQPDLSHIGGIFEARKVAAMAEVYYAAVAPHCPLSAVSLAACLQLAACIPNFLA
                     QEHVSLGEKLLVQPFVIQDGYVGVPDKPGLGIEVDEGKLAELLYDGHWTTPELRHDDG
                     SVADW"
BASE COUNT         2140 a         3673 c         3074 g         1881 t
ORIGIN      
        1 ctgccgagaa cccccctcgc tccagaacct ctttccaacc cttgaccttc cgtcaatccg
       61 aggcataaaa acagcagggt tcaggggaaa gaagccatga gggatacgta caggtactcc
      121 ctgctgctca cgcagagggg acaacgatac acgctggcct tggcggcgct ggccttggcc
      181 gcaggctgct ccaattcaag ccgggatttc cgccccgagg atcaattcgg caggatctac
      241 tacatcgacg gcgccggcaa ctggggtttc ggccaactgg ccgtgccgca gggtttgcgc
      301 gaggccggat atcgcggccg catcgcaacc tatcgctggt cggcgacgct caatccgctg
      361 ctcgaccaga ccgttttccg ggccgtggcc agagcccagg gcagaaagct ggctcgggaa
      421 atcgagcatt atctggcaga atacccaaac aatgaggttc accttatcgc cctctcggca
      481 gggacgggcg tggctgtctg ggcgtgcgag catctgaccc ccccggccaa gatcggtaat
      541 gtgatcctgc tcagttcgag cctttcgtcg gaatacgaga tgagcaaagc actggccaat
      601 gtccggggaa ccatttttgt ctactattcc ccaaatgacg gagtactcgg cgggccggtg
      661 caagtactgg gcacgatcga cggcgcattg ggctcccacc cggccggatt agtcggcctg
      721 cgaggtctgt cgtcgaagat acgcaacatc ccatggaccc gccgctacga agcctatggc
      781 tggacaggaa cgcacgtcag cgccgtgcac cacgatttcg tcaggcacgt tctgtcgcag
      841 catgttcttc ccgaggcgca aatgctgacc gccgaagtcg cgcatctttc agggaccggc
      901 gaggcctcac aacagtgagc ttttacggaa agtaccgcct tcctcaccga aggggcgatg
      961 acggcgtccg accccctacc ggacaaccgc cgcgtagaac ggcaacaagc cgaaccagac
     1021 cggccgctgc caggatcgtg acaaaaggca ttaccggcaa acgatacctg acactcccca
     1081 catagaccat gtggagcagg gtgaacgcgg ctaccggcaa caacaacacc atccacgccc
     1141 ccactgcccg gcggtagccc caccaccccg gaaaggccga cagcaggatg cagatcgtcc
     1201 acagggcccc tgccagcccc gcagcgctgc cgcgatagtt ctcaacattg ggccacgggc
     1261 tccaggtccg ccggaatttg gcccacgcca gccgaaccgc ccgtccagga tcctgccgga
     1321 ttgccgacca tgcttggttg cggaagaagc ggtcccactc aacctcgctc aagccctgca
     1381 cctgcggcat cgtcttggtg tgtgccagat cgctggcccc cgtcgcttgc gactgaaaac
     1441 catcatagag ggaaatgccg cctctggtcg tcaaccatcg ccactctccg atcacccggc
     1501 gatttctggc cgcccagggg ctcagcccga ccgccacaag aagaagcacc gacaccgcgg
     1561 ccgccaggcc ctgccggtca aatccccggg caagcacaat cgccacggca accggcggaa
     1621 ccagaaccac cgccgacggt cgcaacatca ggcacagcca caacatcagg ccggcgccga
     1681 tcaccggggc agggctaacc ctgcgatcgc ggcggcagag cgacaagacg cacgtccatg
     1741 ccagtgccaa cgcaaccaca aacagcgtct cggtgagcag caatccggag aagaacacca
     1801 ggaacggatc gatcgccacc gccagaccgg caagcaccgg taccgccgtg cgcgaaccgg
     1861 cttgctctga cacaaatccg gcaagcgaca gccacccagc agccagaatg tacacgaccg
     1921 gggcaatgaa cgctcccaga attgcttgag tcagtcgagc ccaataagac cgaccgggtg
     1981 agaaagcgtc ggacccgtcc gcgaacagag ccaggaacgc cggatagccc ggcatgtacg
     2041 tagctcgata gccgaactcg tccaccaaac cgtcaccggc ggccagtgat ctggccacat
     2101 cctgataggc cttctcgtca gggtattcgt gcccaggaga agccggaccg ccgtaccgga
     2161 agcacaccca tccggcccgc agaccggcgg cgatcacgaa cagcacgatc aaccagcccc
     2221 atcgccgcat tcgtgagccg caggctgcat tattcatcac gttggcccat tggggactcg
     2281 ctcaatcacg cggagcggac aacggccggg ctgaaagacg catcactcgc cgcgttgctt
     2341 cagtcagacg aacgcgatcg tcgatccgca gcggcaccac ccaaatcgga cccagccgat
     2401 cacacaacaa caccgtccgc tctcgcacgt cggcgtcaat cttctcgtca ataaagaaat
     2461 ccgacaactt cttcagctcg ctcatcccga gcggcaagaa ccggtcaccg ggacggcgca
     2521 accgggcgat cagcggagga tgcacctggt cggcgtcgat ccactcctcc caggacacct
     2581 gcccgcgcga ggccgccgtt cggatgtgct cggcaatcag gctctgatcg gcaaggaggg
     2641 tctcgacgct caactcgatg ccgaatggca acagcgaagt cctgccgtca atgcctacgc
     2701 gtatctcgcc gggaaccggt gccactcccg caggctccgc ttccagttgc agcaccaggc
     2761 gatcgtatct tctgaaaaca cgcaggccgc cgggcaggtc catcgccttg ctgccctccg
     2821 tttcctccgc catcgccgcg acagcggtca gatgcgaata gccgatctcc gcctcgctga
     2881 ctcccagacg caacatcgcc tcacgaatca gttgtgtttt gatgaggcga ggttttcgag
     2941 ccagcgacgt gctcagtagc acgatctgcc gatcgtcgcg ctcgacgatc atcgactcaa
     3001 gcatccgccc cgcggtttcc gtcagatact cgtccacgtc gcgggcttgc tcgcccaggc
     3061 gaacaagtgc ctcggcaacc tgtggattga tcgaatctcg cagcaacggc agtatgtcat
     3121 tgcgaatacg attgcgcgtg tagctgtcgg cgtcgttggt tgcgtcatgg cggaaaggca
     3181 aggcgcggtc gcgaacgtac tcctcgatct cactcctgcg aaacgacaac atgggtcgga
     3241 ccacctgaat accgctgccc ggccggatcg gccgtgagcc ccggatgccg gccaaccccc
     3301 gcaaaccggt accccgcacg attcgatgca ggatcgtctc ggcattgtca tcggcgtggt
     3361 gcgccagcac caccgtgccg gctcccgact tgagacacac ccgctcgaaa aactcgaaac
     3421 ggcatcgccg agcagcctgc tcgatggaga cgccgtcttg ggaagcccgc gcgcgaacat
     3481 cggcggttcc aaccgtgcag tccagaagaa ggccctcggc caactcgcgc acgaacctcg
     3541 catccttatc cgcctcggct ccgcgaagct gatggttgag gtgggcaacg tgaattctca
     3601 gaccccagcc tcgccggttc aatccacaca agagatgaag catcgccacc gagtctgcgc
     3661 cgccggatac ccccgcaaca accgctgccc cccgaggtac caagccgctt cgctcggcat
     3721 cctgggcaaa tcgagattcg agatcctcga cagccatggt cacagccact ccaacgcccg
     3781 gaacgccgtt gtcggctcac cacccggcac cccgctgtca tgaccccgct ccggggggcc
     3841 gaccgcggct ctccccgccg tgttgacggg gctcaatgcc cctcgcggcg atgcccgccg
     3901 acgagccttg ggacagcgaa aaccgctcgt catcatccga tacggctcct cagcctgtca
     3961 gaacctatcc caaaacctgg atgggaccgg cttccagccg tcgggggttc gggataagct
     4021 ttcatctcga aggcgcaagc gtctcgacat aacggacaag ctattttata cctccaagga
     4081 accggttgcc ataacggtcg ctgcaacctg cctttgtgcc ccaatgcggc gcggacgctt
     4141 cgcgggagga acgagacgat gctgtcaaac gcagtattgc tcgccatcgg ctcgctcgga
     4201 tatctcgccg gaccattcac cgtgtacgac ttgcgatatg ccctgcgatt caacgcacag
     4261 aatccgcagg aagtggccgc cgcgtgggat cacgttcacg ccgttgccac gctccagggc
     4321 atcgtgaacc gcgaggaggc caacctttac attctcttcg tcgagtccgg cggcaggtgc
     4381 attgacgagt attggctagg ccgcatgtcg gaacccggcc agtggcttgc cggacgcacg
     4441 cgggcaatgg ttccagacct tggggccctg atcgacaagt ataagaagct cgtcaaaggg
     4501 gccgtcgtct acgaccccgc cgtcccggcc accagcaacc ttgcctcaac catcgccggc
     4561 gtcgagaacc tcatcgccgt ccgctacgac ccttcgccca acagcctcta cagccggctc
     4621 atcacttcgg gtccccgtct gccggtcgtc aggcgcctgc tcaacgagaa cggttcatcc
     4681 atgttcaccg gtcagggtaa catccccggc accgaccagc cgtccaccgg cagcgccaag
     4741 tgcgacgcct atctgtggct caagcaccac tacatcgaca ccggcaaggt cgaagcaggt
     4801 tatgccggct actacatcga ctcgttctgg cagaaatgcc cgaccgccgc cggcccaaac
     4861 cagcacaccc tgagtaacca tgacttcttt gttgcgaaga aggccttctt ttttgacttg
     4921 aacgtctggg ccgacgaaac gccgatcgat gaccgtgccc agaagcccgg taccgatctc
     4981 cagacgttca aggcactcct ccggagcgcc tatgaccgga caagcgggcg gcagatgctc
     5041 cacatcggag gttttacgcc ctgggccttc aaatacacca acgcgggcaa agccggcggc
     5101 acgcacgagc ccgttcccac ggagtgggaa acggccagac tgatcggggc ctacaacgcc
     5161 ttcatcgacg ccgatgccct cggcagtgcc gcgatggcga acgcgtcgtt ctatgcgcat
     5221 tttccgctga acaagcggta tccgcagccg tgggtcacca aacagcggct tatcaaccgc
     5281 ggcctgcttc aaccgaacgg acaggtcaac ttcgacgggc ggcaattcgt gatcttctac
     5341 gtcggggact tcgactcggc cgcgtggctg taccagttcg tacccgtggt ctgggaccat
     5401 ccgggccgcg gcaagttgcc gctcatgtgg tccatcagcc cggtgctcga acgtcgcgca
     5461 ggaatggcca tggactacct ccgccgcacg gccacgccga acgattactt cgccgccgcc
     5521 gacaacgggg ccggctacct caacccgggc atgctccagg agccgcgaga catctccggg
     5581 ttgcccagcg gcctggacct ctgggctgag cactgcactc cgatctacgc ccgctggggt
     5641 atcacgatca ccggctttat catcgacggc tacgctcccg gcttgaataa ggacggcctc
     5701 gattgctacg cgaagttcag ccccaacggc atcgtccccc agaagatccc cgccaccctg
     5761 ctgcacggca acatgccggt tctgagagcc ggttatgatc tgggcgacaa tgccgaacgg
     5821 gccgcgcgaa ccatcgtcgg acgcgtcgcc aggcgaagcg tgccattcca ctggttccga
     5881 ggcattctga aatcgccgga ttggtacatc gaagtctacg accgggcccg cgccgccaat
     5941 ccgaagatcg agctcctcga cgcccctgcc ttcttcgaac tttaccgcgc ctggctcaaa
     6001 tcaaaccccc aggccgccgc cggcaagatc gactgtgaga gataagtctc ttgcccgaag
     6061 gcgcacgtcg gcaggaagcc ggcaggcaga ctggtccgcg cgcagacgct gcctgcttgt
     6121 gatcagagag caagcatctc cgcgcaaggc cgtgggcagg gcacgcggca gcgatgagcc
     6181 gacaggggcg ccccccctcc ttctggttta ttcttcaagc tcgcctcacc tcgcgcttgg
     6241 ccttgtcgta cacgaccttg cagttcaggt cccaggccag gtcgcccaga gcgactgcaa
     6301 cgccctgctt gtggccgaga tccatgtcgg cgttgggccg gccgttgttg cggatgcagt
     6361 cgaaccagtt cttcacgtgg gcggcgccgg gatacggcgg atcgacggtt tctccgagca
     6421 gtttgtgggc ctccagcttg ttgttcgtgc cctcgcctga aaccagcgga ttgccaggat
     6481 ggtggccgaa ccgggtcttg acgcagccct tctcaaacat gaacacggtc gtctcattgt
     6541 caatctcgct gccgaagtgg ctggtgaact gggtgtggaa accctcggcg tagtccagca
     6601 tgaccatggt ctggtccggg gcggtgcact tcgggtcgtt cgtcggggca tagcggccgc
     6661 cccaggcaac cgccgacagc ggataatcgc atccggtcac gaagtgaacg aaggtgatga
     6721 aatgcgtcat ccagcccccg atcgtgccgc tcgtgatctc cttgtatccc atccacttcg
     6781 cgcacatgtt ggcgtcgaac gggcgctccg gccggttgta caaccaggcc ttccagtcgg
     6841 tctcggacac ggttgccggt ccctggtagt gagcccagta aggaacatag gccgtctcgg
     6901 tcgactcgac gcggaaaagc ttgccgagtt tgccctccgc gatcagttcc ttgattgccc
     6961 gaacggccgg tgagctgacg ccgtgcgtgc cgtgctgcac gaccatcttc gaggctttca
     7021 cgacgtcgta gagcctgttc aactcgtgca ggtcaacgtg caaaccgatg atcggtttct
     7081 cgacgtagac atgcttgccg gcctggaccg cgtcgatggc ctgtccggcg tgctgatgat
     7141 ccggcgtggc aatgaccaca gcgtccacgt cggcgtcttc cagcagcttg cgatagtcgg
     7201 cataggcctt gggcttgatc ccgaggatct tctctgcctc ggccgggtaa ctctcccgtc
     7261 gttgcgtcca gatgtcgcca aaagcgacca gggcaaagtt gtcgcccgag cccttggcca
     7321 tttgctcgac gatgaaccgg ccgcgatgac cgcccccgat caggccgaga ttgatacgct
     7381 cgttggcccc gatcacccgg gcaatactcg ctgcgttcaa ccccaaggaa gccgcaccgg
     7441 ccaccgtccc acccaccagc ccctgccgaa tgaacgaacg tcgcgtcagt ctgctgtcta
     7501 tcatgtcgcg ctcctgtgtc gggttctttg cctctttcac ttcgcgcccg ggaccgcacg
     7561 gcggtccgtg gcaagagtgg attctaacga cagccgcacc ttccgccaat ccacccgttt
     7621 ccgcggacaa gcgggacagc cgcttacaac gcaacggtca ctcgccggtc cgctgagtcg
     7681 cgtgccttga gggcgacctc cgtcgcgcgg aaaggatcct cctcactcag aaaatgggga
     7741 cgcaacccgc ggaggctctc gacgaaatca atgaagatgt tgtgactctc gtcggaaagc
     7801 ggcaactggg cagggccctg ttcatcggtg atgagttcgc agaaggtcgt gttggtgatc
     7861 cgcacctcga ccacgcccct ggagccggcc acccgaagac gatcgtcccc gtgacttccg
     7921 gcctttggcg gccggaaata gtcaaacgtc agagtcgcct gccctccgtt gctcatgtca
     7981 aacaacagtg ccccgcaatc ctcgcactca ggataatcct tgtgaacctt caccgcctgg
     8041 cgggcagtga cggaggtgaa atccaagccg gtgacaaacc gtatgaaatc gatggcatga
     8101 atggccaccc atggaatgga accgccgtag ttcttgcgga gcttgaaata ccacggacgc
     8161 ccctggcccc aggcgtagga tttctgggcc gagatcagca gcggctcgcc gatcgaaccc
     8221 tcctggacgg ctttgtgcgc ggccatcagg ccgggatgaa aacgcatcgc cagcatggcg
     8281 gtcacgcgga ctttcgcgcg gtcccgctcg gcccgcaggg cgtcgagatc ctcaagcgtc
     8341 gacgccagag gcttctcgct gatcacgtga cagcctctcc tgacggcttc gatactcact
     8401 tggccgttgt gcgcatacgg cgcgaaggcc gcgacgatgt cgggcctcac ctcatcgagc
     8461 atctgccggt agtcatcgaa tatcctcgtg tcggggttcc aaaccggctt ctccttgagc
     8521 tgctcgaccg gttcgtccgg aaacgaccgg gcaacggcaa ccaggctgca cccttccacc
     8581 ttcgggatgc ctgtaacgat atccccaaaa tggccctgca cgcccacctg agccacccgt
     8641 aacgccttga acgctcgatc cgtcgctgct tgcctggctg attgtgccat gcttgtggtc
     8701 atcccggaca ccatcgccac cgcacctgaa acgaattccc ggcgagtcat catcatacct
     8761 cccgattctc gagacaacga cctgatcata tcgcctgatc cgggcggttg aaaacgttgg
     8821 ccaaaccggg tatcgagtcc cattcatggg ctgcaccgtc gccatgttgc tggtattagc
     8881 tgcgaatccg gggactccct cttcgtcccc agccgcctca cggccgacca ccacccctgc
     8941 gaccgtccgc atcgagtact acagactgcg aaccgcggac ggtcaacatg acgtgcagca
     9001 gtgttccgct ctgcattttg gcaggctggg ccggcgagac gggctctgga tgaccagtga
     9061 ccgcaacggc ggcccgtcgg caggaaaaat ccatttcatc tcagccgcca cgctggcgga
     9121 cgcccaacac caaggtacga tccaggtcga cgaatgcttc acagtcgttc caccggccga
     9181 gggctgggag accttcgcga gcagccattc cgaaataccg gccgaaatcc tggatgacct
     9241 gcacgaccgc gtcgaagccc gcctgaccgg cgataagggc ccctacctcg acctggaagc
     9301 tgtcacgatc gccccttcga tcttgccctc ccacgagact agcctgttcg tggccgccga
     9361 ggaaccctat tcgactgttc tcgaactggt tcctcttgct cacggacccg acgcccaggc
     9421 acagattctg tccttgcata tttacgagga ggcggaggat gagcggggta gcgatcgcaa
     9481 cgacggactt gaggccctcg cgtgggccgg gcaaccgggg ttcttctact gggccgagga
     9541 gggcaccagc tatcacgggg gcgaccccgg accgagactg ttcttccgcg acccgaaggt
     9601 cggccgcggc cggctcatcg ggggacgcct ggccatccag caagatctca cccacaagct
     9661 gacctcggcg gtgtggaccc tcagagaagg acccgagcag accctcaatg cgctgaccgt
     9721 gctgccttcg ggccgtctgc tggccgtcga tcgcaacgga gcctggatcc tgagactcga
     9781 cccgcgggcc ggcaccgccg agcgatggct caatcttcac gacatcaacg ggctcgatct
     9841 gcgaaagctg ctgcacgatt tccccgcccc gcgaaggatg ccatatatca gtatcgaggg
     9901 tatcagtgtg gatccggcgg gcgatatctg gctcatcgac gaccccgccc tgcccgaggc
     9961 cttccgcgcc tcgtgcctga ttcggcttcg cggcctgaat cctgatgcgg ccggcccgtc
    10021 acaggaaaaa cgatggcgtg cagacacgga tcaatcaagt gctgaccgcc gaaagctgat
    10081 cgccgacggc ctttactacc aatccgccac tgacccgtcg tcgtgtcgca gttcaggcgt
    10141 cgtccaatgg ccgtcgtaaa gaagttccgc cagcttgccc tcgtcgacct caatgccgag
    10201 accaggttta tccggaactc ccacgtaacc gtcctgaatg acgaagggct ggacgagcag
    10261 cttctcgccc aggctcacgt gttcctgggc caggaagttg gggatacacg cggccagttg
    10321 cagacatgct gcaagggaca ccgcgctcag cgggcagtgc ggcgcgacgg ccgcatagta
    10381 cacttccgcc atcgccgcca ctttgcgggc ctcgaagata ccgccaatgt gcgacaggtc
    10441 cggctggatg atggccgccg cctgcttctc caacacctcg cgaaaccccc acctggtgaa
    10501 caaccgctca cccgtggcga tcgggatcga cgtcgagcgg gcaacagtga ccatggtgtc
    10561 gacgttttcg ggcagacacg gttcctccag gaacatcggg tggtacggct ccagggcctt
    10621 cgcaaaacga atggccatgg ctggactgag gcggccgtga caatcgagcg ccagatcaaa
    10681 cccgctcccc gcttcctcgc ggactgccgc catcaggtcg accgcccgct caaccacggc
    10741 cggcgaatcg acgatcttcg tcgcccac
//
LOCUS       DAOTPW010000198        10758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5757_length_10758_cov_5.484070, whole genome shotgun sequence.
ACCESSION   DAOTPW010000198 DAOTPW010000000
VERSION     DAOTPW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5757_length_10758_cov_5.484070"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="P5316_16085"
     CDS             complement(<1..1160)
                     /locus_tag="P5316_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRR86530.1"
                     /translation="MNFLAPWHVPVLAAALALPPLVLLYFLKLKRRQIPIASTLLWKR
                     AVHDLQVNSPFQRLRNNLLLILQLLILIAAIAAISEPVRPGKRGIDKAMVLLIDQSAS
                     MGAKEADGRTRLQIAKEEAHKIVNELGSSQRAMIIAFCDRARVMSAFTNDKPQLRAAI
                     DAIEQTDGPSLLREAMELAEAHSTPIGEVREVGSSQYILLTDGRLPDSESVRVERGTM
                     EIARIGEATENVGIVSLDVRRNYDRPEQISALINVRNFGTRPAKRDISLLVDGQIKNV
                     QDLPELAPYGRPDAIHAAKYTKDPADNSQATAAFEMMLDTAATIEVRLSGEDALAIDD
                     RAFAVVTPPRSIKALLVSPGNRFLRIAIGAVLDSFDIWQPEEYEKASDDKLI"
     gene            complement(1157..2053)
                     /locus_tag="P5316_16090"
     CDS             complement(1157..2053)
                     /locus_tag="P5316_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRR86531.1"
                     /translation="MALATKNNQLLDPDFMNQLDRLDIVSRKIFAGKMKGERRSKRKG
                     QSVEFADYRSYVIGDDLRFLDWNIFARLERLFIKLFFEEEDLHVNVLIDCSRSMDFGE
                     PDKALYARRVAAAIAYIGLVNYNRVTLTAFSDRYGPEMAGIRGRRLIHQVLGFLGGLN
                     AEGAGNMTGACKQFAIRHPQRGVCIVLSDFMDKTGYEAGLRYLIGRKYDLYVIQLLSP
                     EEIDPTLTGDLKLLDVEDNDTAEITVSRALLNRYKQTLRAYCGGLRDFCTKRGAVYLF
                     TSTAVPFDQLVLTYLRQRGLLK"
     gene            complement(2180..3199)
                     /locus_tag="P5316_16095"
     CDS             complement(2180..3199)
                     /locus_tag="P5316_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRR86532.1"
                     /translation="MAETIDPRVEKATREFCHKFQRLREEIGKIIVGQQEIVDGVLVC
                     LFTGGHALLEGVPGLGKTYLIRTLAQALSLNFSRIQFTPDLMPADIIGTTIITDDPVT
                     GKRDFRFQRGPLFAQIVLADEINRATPKTQSALLEAMQEHTVTVGGEKHALQEPFFVM
                     ATQNPIEQEGTYPLPEAQLDRFFFKLIVNYSSREELKTILDRTTTGYDAKIEPVMTAE
                     EIIGAQRLVRRVVIAPHVQDYAVRMTLATHPDGEFATEMTNKYVRWGSSPRGAQAVTL
                     AAKVQALLEGRFNVSFEDVRKVALPTLRHRVLLNFEGEAENISTDDVIRDILKQTPTL
                     TEQAA"
     gene            complement(3267..6467)
                     /locus_tag="P5316_16100"
     CDS             complement(3267..6467)
                     /locus_tag="P5316_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRR86533.1"
                     /translation="MALAFNWPLSFEQPAWLWLLLVIPAIAVISRRSLAGLEPHRRIL
                     TVVFRSAVIVMLALALARVQFVRRNEHVAVMFVIDKSHSIPDDRRTAAEQYVRKVINR
                     TSRDDRVGVITFDGKADVDLIPSRGGDDQLGNNIGFGMTVQPDASNIAGGVRLALAAL
                     PEGFKRRIVLITDGNQNVGNIEEEIGAATASGVAIDVIPLTYSHDSEILLDRIVVPSQ
                     AAKDTRLPIRVIAKSKKPTRARLTLYHNGQTVPLPEAEFDIAGGMQPTVITTSVEIFS
                     GGFHIFDARLTALDQSADKIMENNRATAFSFVNEQGGVLLLARNDEKADHDLLLAALK
                     REKVDVTLETVEDLSIDLNTLQQYGVVILSNLSADTFNEEQHKALASYVRDSGGGLIM
                     IGGEQSFGAGGWIGKPVEEVSPVNFDIRQKKVIPRGALAIIMHSCESPQGNDYGEKAA
                     IATVETLSSQDYLGVITYNWSVGGANWDVPMSLLSDKSAVIRKMKNLQIGDMPDFGTA
                     MQMAINGLLQAKSKGASQLHMIIISDGDPQAPSNAVIDQMNKNKITASTIGIGYGGAH
                     CVETTLIDIARRTSGDPRKFFRCTNPRTLPQIFIKEAKVIRRTLLSEERFTPAVAQYD
                     MQTVQGIAEGPIPQLGGLVLTEPKADIIMPLVRKSADGTNDPLLAYRRFEMGKMAVFT
                     SGLWKKWGAGWAQWEKFGRFWAQLINWSMRQSSGMDFDVTTRLEGNTGRIVVEALKKD
                     ASYLNFLQIGGRLVLPNMETEDLHLVQTGPGQYEATFDVVDTGNYLINLKYTDPDKTT
                     GLILSGLSVPYSQEFRVLQTNTPLLERIVTRSGGRKLAMDPVNDKVFDPRGLPPAEMR
                     QPVWRWVISWLLIPLFLLDVAVRRLASTLAMSIYVELAVLAVLIGILYTAGAGFWGYL
                     GALVFAELVGWTMRRRYIIPTIGFFTSSVTSLARAGQRSAQSLSHLKGVRDKVRGDLT
                     AKPDADTRPGTVTIPLEPAADPKKRFEVDEETAAKPAGDLTESLGGAVAAEIDEPKKP
                     SQRPGRKPTGQEDVTGRLLKAKQRARDQIEDEKKES"
     gene            complement(6471..7466)
                     /locus_tag="P5316_16105"
     CDS             complement(6471..7466)
                     /locus_tag="P5316_16105"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86534.1"
                     /translation="MKLLTDIATHDGANHPNNHLSTRLATRTKLSTLLLAIAIAGGMV
                     GCQKRGISGRTPERIIGSHGFGPGQFHRPRAIATAPDGCVFVVDMTARIQRFSPDGEF
                     EVVWRTPESENGKPTGITIDAQGRVLVADSHYHRIIIYDRDGRELARFGQGGTGPGQF
                     DLVAKAVVDPQGMVYVSEYGLTSRISRFSPDFKFLGSFGGPDSGPASLSRPQSMAFDN
                     DGTLWVADACNHRICRFSRDGKLLFTFGGPGYEPGRLQYPYGLAVCDDGTLLVVEFGN
                     NRLQRFDRAGKSLEVWGKPGTQPGQMLDAWSVAVGKQGRVYVVDCRNHRVQMFRM"
     gene            complement(7471..9066)
                     /locus_tag="P5316_16110"
     CDS             complement(7471..9066)
                     /locus_tag="P5316_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86535.1"
                     /translation="MARLLVIGLCAVGWLVLAGGIVAPLAGVFLAAGQSGAISTELVA
                     VPAWQVLLLRSLVMSLTTTVAAVALGLLPAAVLGSASPRRLPTLVGLALLPLLVPPQV
                     YAYAWQLVSAPASPLRRFMPDNVSDMWFGGAIRAGLISAGWLWPLSAMILAAGWRSAG
                     RTAFALALLDAGPTRAYLRAVVPSLRPHLMAAATTVFAVSLLEYAIPHLTRCRIYATE
                     LLVLVDAGAPAGQIIRMAVQVIAILMVLVALVVWIIRPTAHWGQVGMDDETANLARPG
                     RTGIQANTGRLTWAGAALVWVTTLGVPVGIMSALKPGSSSWAASFSILSREWLSSLSI
                     ALAAGMAAIAMASGTAMLDAAARSRWARTGGWTTFLAAAIPPAALGVGFILIYNRPGF
                     VGYLYTDTPCVWILSLVSRYGALAVLIVWLTVGRQGNAVADQARADGAGAAGILAHVL
                     LPMVWPSLLAAGLIVTMLAMFEVVVTQMTGPVGYPSIAVTLLGQMHYGRDNVVITTSQ
                     TVVVAGFVLTQICGRLLMAARER"
     gene            9376..9663
                     /locus_tag="P5316_16115"
     CDS             9376..9663
                     /locus_tag="P5316_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86536.1"
                     /translation="MLLASVLVVVIAAVALRTVVKKRMKEEILEAVREPVVPVCYKCG
                     YDMRGQIFPRCPECGALLGFTVSTDELPLTDEERQLIEDRCRGIRRKEPEQ"
     gene            complement(9963..>10758)
                     /locus_tag="P5316_16120"
     CDS             complement(9963..>10758)
                     /locus_tag="P5316_16120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRR86537.1"
                     /translation="NLKFMAGLPDACCDLIYADPPFFTGKTKKQADGKHRFNDSWEGD
                     LDSFLNFLRPRLQHMHRLLRPTGSLYLHLDWHAVHYAKVMLDEIFGGDHFCNEIIWSY
                     RTGGHATRWFARKHDTILFYARQAGRQKFHVQREGRFRTDGLNYDEQGRPYKQTRAGR
                     LYFNSEGPAMTDVWEIPFLSTVSLERTGYPTQKPEALLERVIKASSDPGDIVADFFCG
                     SGTALAVAQRLGRRWLGCDINPHAVDLARHRMTTDQPLLPFPETEG"
BASE COUNT         1870 a         3153 c         3577 g         2158 t
ORIGIN      
        1 tcgatcagtt tgtcgtcgct cgccttctca tactcttcgg gctgccagat gtcgaagctg
       61 tcgagcacgg ctccgatggc gattctcagg aaacggttac cagggctgac gagcagggcc
      121 ttgatcgatc gcggcggagt gaccacggcg aaggcccggt cgtcgatggc cagcgcgtct
      181 tcgccggaga gccggacttc gatcgtggcg gcggtgtcga gcatcatctc gaacgcggcg
      241 gttgcctggc tgttgtcggc gggatccttg gtgtactttg cggcgtggat cgcgtcaggt
      301 cgcccatagg gggcgagttc cggcaggtcc tgtacgttct tgatctggcc gtcgaccagc
      361 aaggagatgt cgcgtttggc gggcctggtg ccgaagttcc tgacgttgat gagggcgctg
      421 atctgttcgg ggcgatcgta gttgcggcga acatcgaggc tgacgatgcc cacgttctcg
      481 gtcgcctcgc cgatgcgggc gatttccatg gtgccgcgtt cgacgcgcac gctttcagag
      541 tcgggcagcc gcccgtcggt caacaggatg tattgcgagg aaccgacctc cctcacctcg
      601 ccgatggggg tggagtgggc ttccgccagt tccatcgcct cgcgcagcag gctggggccg
      661 tcggtctgtt cgatagcgtc gatcgccgcg cggagctggg gcttgtcgtt ggtgaatgcc
      721 gacatgactc gggcccggtc gcagaaggca atgatcatgg cgcgctgcga ggagcccagt
      781 tcgttgacga tcttgtgagc ctcttccttg gcgatctgga ggcgggttcg gccgtcggcc
      841 tctttcgcgc ccattgaggc ggactggtcg atcagaagga ccatggcctt gtcgattccg
      901 cgcttgccgg gccgcaccgg ctcgctgatg gccgcaatcg ccgcgatcag gatgagcagt
      961 tgcaagatga gcagcaggtt gttacgcagc ctctggaacg gggagttgac ttgcagatca
     1021 tggaccgccc gtttccagag caatgtcgag gcgatgggga tctggcgacg cttgagcttg
     1081 aggaaataga gcaggaccag cggcggcagg gccagggcgg cggccaggac cggaacatgc
     1141 cagggcgcga ggaagttcat ttcaacaacc ccctttgccg cagataggtg agcacgagct
     1201 ggtcgaacgg cacggcggtg ctggtgaaca gatacactgc accgcgtttc gtgcagaaat
     1261 ctcggagccc accgcaatat gccctcaggg tctgcttgta gcgattcagc aaggcccggc
     1321 tgacggtgat ctcggcggtg tcgttgtctt cgacgtcgag gagcttgagg tcgccggtga
     1381 gtgtggggtc gatttcctcg ggggagagga gttggatgac gtagaggtcg tacttgcgtc
     1441 cgatgaggta tcggagaccc gcctcatagc cggtcttgtc catgaaatcg ctgagcacga
     1501 tgcagacgcc tcgttgcggg tgccgaatgg cgaactgttt gcacgccccg gtcatgttgc
     1561 ctgcaccctc agcgttgagg ccgccgagga agccgagcac ctggtggatc agccggcgtc
     1621 cgcggatgcc ggccatttcc ggcccgtagc ggtcggagaa ggccgtaagc gtcacgcggt
     1681 tgtagttaac cagaccgatg taggcgatgg cagcggccac ccgccgggcg tagagggcct
     1741 tgtccggctc gccgaaatcc atcgagcggc tgcagtcgat cagcacgttg acgtggaggt
     1801 cttcttcctc gaagaagagc ttgatgaaca gccgctcaag ccgggcgaag atgttccagt
     1861 cgagaaagcg caggtcatcg ccgatgacgt agctgcggta atcggcgaac tcgaccgatt
     1921 ggcccttgcg tttcgagcgt cgctctccct tcattttgcc ggcgaagatc ttgcgcgaca
     1981 cgatgtccag ccgatcaagc tggttcatga agtcggggtc gagcaactgg ttgtttttcg
     2041 tcgcaagggc cataagaagc tctcagcttt cagcggtcag ccgggggcag taacacctga
     2101 tcgaagcatc attcatcgtt ccgccttcat tcttcactga actccgaacc ctcaaaggcc
     2161 gtcgagggca tcggtcctgc tacgccgcct gttccgtcag cgtcggcgtt tgtttgagaa
     2221 tgtccctgat cacgtcatcg gtgctgatgt tttcggcctc tccctcgaaa ttcaggagca
     2281 cgcgatggcg caatgtcgga agggccactt tcctcacgtc ttcgaatgac acgttgaaac
     2341 ggccctccag caaggcctgt accttagccg ccagggtgac ggcctgtgct ccgcgcgggc
     2401 tcgaacccca tcgcacgtat ttgttggtca tctcggtggc gaattcgccg tcaggatggg
     2461 ttgccagagt cattcgtacg gcgtaatcct gcacgtgcgg cgcgatgacc acgcggcgca
     2521 ccagtcgctg ggcgccgatg atctcctccg cggtcatcac cggctcgatc ttggcgtcgt
     2581 aaccggtggt cgttcggtcg aggatggtct tgagttcttc gcgagaggag tagttgacga
     2641 tcaatttgaa gaagaagcgg tcgagctggg cctcgggcaa gggataagta ccttcctgtt
     2701 cgatggggtt ctgcgtggcc atcacaaaga agggctcttg gagggcgtgt ttctcgccgc
     2761 cgacggtgac ggtatgttcc tgcatggcct ccagcagggc tgactgtgtt ttgggcgtgg
     2821 cccggttgat ttcgtccgcg agaacgatct gggcgaagag cggtcccctt tgaaagcgga
     2881 agtcccgctt tcccgtcacg gggtcatcgg taatgatcgt ggtgccgatg atgtcggcgg
     2941 gcatcaggtc gggcgtgaac tggatgcggc tgaagttcaa actcagggcc tgggccagcg
     3001 tgcggatcag ataagttttg cccagaccgg gcacgccctc gaggagggca tgcccgccgg
     3061 tgaacaggca gaccagcacg ccgtcgacga tttcctgctg gccgacgatg atcttgccga
     3121 tctcttcgcg cagccgctgg aacttgtggc agaactcgcg cgttgccttt tcaactcgtg
     3181 ggtcaattgt ctcggccatc gcatgtacct ccgtctggaa tgccggtgag cctcgggtca
     3241 accgtcttgt cgcgtcgatc cggaagtcac gactctttct tttcgtcctc aatctggtcc
     3301 cgcgcgcgct gcttggcctt cagcaggcgg ccggtcacgt cttcctgtcc ggtgggcttg
     3361 cggcccggtc tttgtgacgg cttctttggc tcatcgattt ccgctgccac ggccccgccg
     3421 agcgactcgg tcaggtctcc cgccggtttg gctgcagtct cttcatccac ctcgaatcgc
     3481 ttcttcgggt cggcggccgg ttcgaggggg atggtgaccg tgcccggccg cgtatccgcg
     3541 tccggcttgg cggtcagatc cccgcggacc ttgtcgcgaa cccccttgag gtgggaaagc
     3601 gactgggcgc ttcgttggcc ggcgcgtgcc agggaggtca ccgacgaggt gaagaaaccg
     3661 atcgtcggaa tgatgtatcg ccggcgcatg gtccagccga ccagttcggc gaacaccagg
     3721 gcgccgaggt atccccagaa gcccgcgccg gccgtgtaga ggatgccgat cagcaccgcc
     3781 aacaccgcga gttccacata gatggacatg gccagcgtcg atgccagccg tcgcacggcc
     3841 acgtcgagca ggaacagggg gatgagcagc caggagatga cccatcgcca gacaggctgc
     3901 cgcatttcgg cgggcgggag gcccctcggg tcgaagacct tgtcgttgac cgggtccatt
     3961 gccagctttc gaccgcccga ccgggtcacg attcgttcga gcagcggcgt gttggtttgc
     4021 agcacgcgga actcctgcga gtagggcacg ctgagtccgc tcaagatcag gccggtagtc
     4081 ttgtccgggt cggtgtactt gaggttgatg aggtagttgc ccgtgtcgac tacgtcgaag
     4141 gtggcctcat actggccggg tccggtctgc acaaggtgca ggtcttcggt ttccatgttg
     4201 ggcagcacca gacggccgcc gatctgcagg aaattcagat aagatgcgtc tttcttgagc
     4261 gcctcgacga cgattctgcc cgtattgcct tccagccggg tggttacgtc gaaatccatc
     4321 ccggacgact ggcgcatgga ccagttgatg agttgtgccc agaagcggcc gaatttctcc
     4381 cattgtgccc atccggctcc ccatttcttc cacaggccgc tggtgaacac ggccatctta
     4441 cccatttcga atcgccgata ggccaggaga gggtcgttgg ttccgtcggc actcttgcgg
     4501 accaggggca tgatgatgtc cgccttgggc tcggtcagga ccagcccgcc caactgcggt
     4561 atgggtcctt cggcaatacc ctggacggtc tgcatgtcgt attgggcgac ggcaggcgtg
     4621 aagcgctctt cggacagcag cgtccggcgg atgaccttcg cctccttgat gaagatctgg
     4681 ggcaacgttc gggggttggt gcagcggaag aacttgcggg gatcgccgga ggtgcgccgg
     4741 gcgatgtcga tgagcgtggt ctcgacgcaa tgtgctccac cgtagccgat accgatggtc
     4801 gaggcagtga ttttgttctt gttcatctga tcgatgacgg cattgctcgg ggcttgcgga
     4861 tcgccgtcgc tgatgatgat catatgaagc tgagaagcgc ccttgctttt ggcctgcagc
     4921 agtccgttga ttgccatctg catggcggtg ccgaagtcgg gcatgtcgcc gatctgaagg
     4981 ttcttcattt tcctgatgac cgcggacttg tccgagagga gggacatcgg cacgtcccag
     5041 ttggccccgc cgacgctcca gttgtaggtg atgaccccca agtagtcttg cgaactgagc
     5101 gtttcgacgg tggcgatggc ggccttttcg ccgtagtcgt tgccctgggg gctctcgcaa
     5161 ctgtgcatga tgatcgcgag agccccgcgc gggatgactt tcttctgccg gatgtcgaag
     5221 ttgaccgggc tgacctcctc gacgggcttg ccgatccagc cgccggcccc gaacgactgc
     5281 tctccgccga tcatgatgag gccgccgccg gagtctctca cgtagctggc gagggccttg
     5341 tgctgttcct cgttgaacgt atcggccgac aggttggaca gaatcacgac gccgtactgt
     5401 tggagggtgt tcaggtcgat gctgaggtcc tcgacggttt cgagcgtcac gtcgaccttc
     5461 tcccgcttga gggcggccaa cagcaggtca tggtcggctt tctcgtcatt cctggcgagc
     5521 agcaggactc ctccttgctc gttgacgaag ctgaaggccg tggcccggtt gttctccatg
     5581 atcttgtcgg cgctctggtc gagcgccgtc aaccgggcgt cgaagatgtg gaatccgccc
     5641 gagaagatct ccaccgatgt ggtgatgact gtcggttgca tgccgccggc gatatcgaat
     5701 tccgcttcgg gaaggggcac ggtttggccg ttgtgataga gcgtgagcct ggctcttgtg
     5761 ggtttcttgc tcttggcgat gacgcggatt ggcaggcgtg tatccttggc ggcttggctg
     5821 ggcacgacga ttcggtcgag gaggatctcg ctgtcgtgcg aatacgtgag cggaatgacg
     5881 tcgattgcca cgccgctggc cgttgccgcc ccgatctctt cctcgatgtt gccgacgttc
     5941 tgattgccgt cggtgatgag cacgattcgc cgcttgaagc cttccggcag ggcggcgaga
     6001 gccagccgca cgccgccggc gatgttggac gcatcgggct ggacggtcat gccaaagccg
     6061 atgttgttgc ccagttggtc gtcgccgccg cggctgggga tgaggtccac gtcggctttt
     6121 ccatcgaagg tgatcacgcc gacgcggtcg tcacgggagg ttctgttgat cactttgcga
     6181 acgtactgct ccgcggccgt gcggcgatcg tcggggatgc tgtgggactt atcgatcaca
     6241 aacatcaccg cgacgtgctc gttgcggcgt acgaactgga cgcgggcaag ggccagggcc
     6301 agcatgacga tgacagccga ccggaagacc accgtgagga tgcgtcgatg cggttcgaga
     6361 ccggccagag atcgccgcga gatgacggcg atggccggta taaccagcag cagccacagc
     6421 cacgcgggtt gctcgaaaga caaaggccag ttgaacgcca aggccatcag ctacatcctg
     6481 aacatctgga cgcggtggtt acggcagtcc acaacgtaca cgcggccttg cttgcccacg
     6541 gccacgctcc acgcgtcaag catttgtccc ggttgtgtgc ccggtttgcc ccaaacctcc
     6601 aggctcttgc cggcgcgatc gaaccgctgc aagcggttgt tcccgaactc aacgaccagc
     6661 agggtgccgt cgtcgcagac ggccagtccg taaggatact gcaaccggcc gggctcgtag
     6721 ccggggccgc cgaaggtgaa gagcaacttg ccgtcgcggc tgaaccggca gatccgatgg
     6781 ttgcacgcgt cggcgaccca gagggtgccg tcgttgtcga aagccattga ctgagggcgg
     6841 gacagcgagg cggggccgct gtcgggaccg ccgaaagatc cgagaaactt gaagtccggc
     6901 gagaagcggc tgatgcggct tgtcaggcca tattcactga cgtagaccat gccttgggga
     6961 tccacaacgg ccttggcaac gagatcgaac tgacccgggc cggttcctcc ctggccgaac
     7021 cgggccagct cgcggccgtc gcgatcataa atgatgatgc ggtgatagtg cgagtcggcc
     7081 accaggacgc gtccctgggc gtcaatggtg atgcccgtgg gcttgccgtt ttcgctttcg
     7141 ggggttcgcc agacgacttc gaattcgccg tccgggctga atcgctgaat tcgggcggtc
     7201 atgtcgacca cgaacacgca tccgtccgga gcggtggcga tagcccgcgg gcgatggaac
     7261 tggcccggtc cgaatccgtg gctaccgatg attctttcgg gtgtgcggcc ggaaataccg
     7321 cgcttctggc acccgaccat tccgcccgcg attgcgattg ccagcagaag tgtggacagc
     7381 ttggttcgtg tcgcgagccg ggtggaaaga tggttgttgg ggtgattggc cccgtcgtga
     7441 gtcgcgatgt ccgtcaagag cttcacgggg ctacctttcc cgggccgcca tcagcagccg
     7501 gccgcatatc tgggtcagga cgaacccagc gaccaccacg gtctggctgg tggtgatgac
     7561 cacattatcc ctgccgtagt gcatctgtcc aaggagcgtg accgcgatgc tagggtagcc
     7621 caccggtccg gtcatctggg taaccaccac ctcgaacatc gccagcatcg taacgatcaa
     7681 gccagccgcc agaagcgacg gccagaccat gggcagcagc acatgggcca gtatgcctgc
     7741 cgccccggca ccgtcggccc gggcctgatc cgccacggcg tttccttgcc ttccgacggt
     7801 cagccagacg atcagcaccg ccaaggcgcc gtaacggctt accagcgaca gaatccagac
     7861 acagggggtg tcggtgtaca agtatcctac gaaccccggt cggttgtaga tcaggatgaa
     7921 gccgactccc agcgcggcgg gcgggattgc cgcggccaga aacgtcgtcc aaccgcccgt
     7981 tcttgcccat cgggatctgg cggccgcgtc gagcatggct gttcccgatg ccatggcaat
     8041 cgccgccatg ccggccgcga gcgcgatgct cagtgaggaa agccattccc ggctgagaat
     8101 cgagaaactc gccgcccacg atgaactgcc cggcttcagc gccgacatga tgcccacagg
     8161 gacacccagc gtggtcaccc aaaccaatgc cgctccggcc catgtcagcc gccctgtatt
     8221 ggcctggatg cctgtcctgc cgggccgggc gagattcgcg gtttcatcgt ccattccaac
     8281 ttgcccccaa tgggcggtcg gccgaatgat ccacacgacg agggccacca gcaccatcag
     8341 gatagcgatg acctgcacgg ccattcggat gatctggccc gccggggcac cggcgtccac
     8401 taggaccagc agttcggtgg cgtaaatccg acacctcgtc aggtgcggga ttgcgtattc
     8461 caggagtgag acggcaaaga ccgtcgtggc ggccgccatg aggtggggtc gcaggctggg
     8521 aaccactgcc cgcagatacg cccgcgtggg tcctgcatcg agcagagcca gcgcgaaggc
     8581 cgtacggcct gcggagcgcc acccggcagc caagatcatc gccgacagag gccataacca
     8641 tccggccgat atcaggcccg cccggatggc tcctccgaac cacatgtccg agacgttgtc
     8701 cggcatgaac ctcctcaatg ggctggccgg ggcggatacc agctgccacg cataggcgta
     8761 gacctgcgga ggaaccagca gcggcagcaa tgctagtcca accagcgttg gcagtcgccg
     8821 gggcgaggcc gaaccgagta cggccgcggg cagaagaccc aatgcaacag ccgcgaccgt
     8881 ggttgtcaac gacattacca ggcttcggag caggaggacc tgccatgcag gcaccgccac
     8941 cagctcggtc gagattgccc cggattgccc tgctgctaga aagaccccgg ccaggggtgc
     9001 aacaatgccc cctgccagca ccagccagcc gaccgcgcag agcccgatga ccagaagcct
     9061 cgccatgtac tccgtccgtt ttcggcggac ctgccaccac tattgacgct caagaggcaa
     9121 taacgtttcg acggacaccc gtaaacccct gacgattcca gcgaagatat gataccgccg
     9181 tggcggccgg gttgcatgcg ggccgaccgc gacagatcgt ccctgcgggc gggatgcccc
     9241 cagcgttacg tgcggcaacg ccgggtcttg ctcgtgttca gtcgcgatct tggtgccccc
     9301 tgctttaggt tgcccatcct gggggataga ctgtgggtta tgtccgtctg gacgttgctc
     9361 tggtggattg gcgggatgct tctcgcttcg gtgttggtcg ttgtgatagc tgcggttgcg
     9421 ctgcgcaccg tggtgaagaa acggatgaaa gaggagatcc ttgaggccgt acgcgagccg
     9481 gtcgtgccgg tgtgttacaa gtgtggctat gacatgcgcg gtcagatctt tcctcgttgc
     9541 ccggagtgcg gggctttgct gggttttacg gtctcgacgg atgaactacc actgaccgac
     9601 gaggagaggc agctcataga agaccgctgc cggggcattc gtcgaaagga gcccgagcaa
     9661 tagggtggag ggtacgtttt tccggcggga ttcgcactcc gggctgccga ttcaggatgg
     9721 atggtcaaga cagggaggct cgccggcgga gcggtgttca gcgaggccag acggtgttgg
     9781 catggagatc gctctttttg ccgagcgagg aacatgtgat cccggtgtgt ggtcccgggc
     9841 acggcgttcg gcaacggatg agtgtgttgg acgcattcac cgatgaccgg gaagccggtc
     9901 acccaggggt ttgtctcgac gcggggttgc ggttggacag aatggtgcaa gagcggcgat
     9961 cgttacccct ctgtttcggg aaagggcagt agcggttgat cggtggtcat gcggtggcgg
    10021 gcgaggtcga cggcgtgggg gttgatgtcg cagccgagcc agcggcggcc gaggcgctgg
    10081 gcgacggcca gggcggtgcc gctgccgcaa aagaaatccg cgacgatgtc accggggtcg
    10141 ctgcttgctt tgatgacgcg ttcgagcagg gcttcgggtt tctgtgtcgg gtaaccggta
    10201 cgttccagcg acacggttga gaggaagggg atctcccaca cgtcggtcat ggccgggccc
    10261 tcggagttga agtagagacg gccggctctg gtttgtttgt acgggcggcc ctgctcgtcg
    10321 tagttcaggc cgtcggtgcg gaatcgcccc tcgcgctgga cgtggaactt ctgccggccc
    10381 gcctggcggg cgtagaagag aatggtgtcg tgcttgcggg cgaaccatcg cgtggcatgg
    10441 ccgccggtgc ggtacgacca gataatctcg ttacagaagt ggtcgccgcc gaagatctcg
    10501 tccagcatca ctttggcgta atgaacggcg tgccaatcga ggtggagata cagcgagccg
    10561 gtcggtctga gcaaacgatg catgtgctgc aggcgcgggc ggaggaaatt gagaaagctg
    10621 tccaggtcgc cttcccaaga gtcgttgaac cgatgtttgc catcggcttg cttcttggtc
    10681 ttgccggtga agaaaggcgg gtccgcgtag atgaggtcgc agcaggcgtc cggcaggccg
    10741 gccatgaatt tcaggttg
//
LOCUS       DAOTPW010000199        10645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5828_length_10645_cov_5.153824, whole genome shotgun sequence.
ACCESSION   DAOTPW010000199 DAOTPW010000000
VERSION     DAOTPW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5828_length_10645_cov_5.153824"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1747)
                     /locus_tag="P5316_16125"
     CDS             complement(41..1747)
                     /locus_tag="P5316_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRR86538.1"
                     /translation="MATTEGELFRTFIHDIIDEDMRTNRWGGRVVTRFPPEPNGYLHI
                     GHAKSICLNYGLARDYGGKFNLRFDDTNPVKEEQEYIDSIIEDVRWLGADWEDRLFFA
                     SDYFQQMYEWAVQLIKAGKAYVCDLSADQVAAMRGTATEPGQESPYRNRSVEENLDLF
                     ERMKKGEFPDGSRTLRAKIDMASPNFNMRDPVMYRILHATHPHTGDEWCVYPMYDWAH
                     GLEDSIEQVTHSICTLEFENHRPLYDWFLDQLGIYHPQQIEFARLNLTYTVMSKRKLL
                     QLVKEGLVRGWDDPRMPTICGLRRRGYTPEAIWNFCRRIGVNKYDSIVDIQLLEHCIR
                     EDLNKRAPRVMGVLRPLKVVIDNYPDGQVEELDAINNPEDPGAGTRHVPFSKVLYIEQ
                     DDFREVPPPKYFRLTPGQEVRLRYAYFVKCTGCVKDPATGEVSEVHCTYDPATRGGDS
                     PDGRKVKGTIHWVSAGQAVDAEVRLYDHLFSKPDPDDVPEGQDWKTGLNPNSLEVLTG
                     CKVEPSLANAAPGSRFQFERMGYFCVDPDSTPGKPVFNRTVTLKDTWAKIAGKQEGKP
                     KR"
     gene            2315..3106
                     /locus_tag="P5316_16130"
     CDS             2315..3106
                     /locus_tag="P5316_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRR86539.1"
                     /translation="MQIRNRRDWLVAATLLGLVATGGCGISPAIKRAGRTDEQKQQLI
                     KPGMFGDNAPADRLRGRFDFWNEEVIRQDVAVGTVFMGDSITELWELAAYFAPSDGVI
                     LNRGIGGDITPHMARRFEADVIQLKPRNVVILAGTNDVARMLSAKKTEDETMSQVMTN
                     MEAMVDAAQKAGINVFICSILPTNPDTGNHAGKAPILPKINEKLKAMCAAKHCIYVDY
                     AAHMSNSAGELPRDLARDGLHPHYAGYAIMARVLNDAARANGLRL"
     gene            complement(3194..3475)
                     /locus_tag="P5316_16135"
     CDS             complement(3194..3475)
                     /locus_tag="P5316_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86540.1"
                     /translation="MTSHLVRLGTLLGLAAITGLSSTAMAARPQFAYRPPAMRSAVPP
                     IIVKKVQPGPVIIKPSQAVIIPIPTTWDAVMMPIPTDIDAKIVLLRTKP"
     gene            4034..5221
                     /locus_tag="P5316_16140"
     CDS             4034..5221
                     /locus_tag="P5316_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86541.1"
                     /translation="MPATASKPASGPAPLLSSPAIERIPLPVQGKSGPAFYVLRKPAG
                     YGSGQLPPLIVALHGTDDTAEQTVGFWAARSMRIPPLITAPQGVGPGWRNSDTAVIHA
                     MFKHLRANVSYDAQRVLLTGFSAGGAMSFELLYVEEVPVTAVAALANYVPPWITGEQV
                     KAKAHVPVFYAVGIADINHEQMREGLQRLRTAGARIDLDRPRIGHVLDSDVAQEAIDW
                     FFDRCADQVGAALDRMAAARLVADLPALDRICLQRRWHEPLHVRRAEQLIKEIEAPGR
                     LELARARAFMAEGRAADAVEVLQEIEGRFVAGPLAREAKALRLHLESDPAVRREIADR
                     LAGRRAEQALSMYVNAQRLVVQRQFDEAARQCRRIIDLYGETPGAERARTLLQLLENR
                     KKQ"
     gene            5218..5883
                     /locus_tag="P5316_16145"
     CDS             5218..5883
                     /locus_tag="P5316_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR86542.1"
                     /translation="MSRNDLRIKAFVDPSFAENAYVVSVSSDDGNRVGWVIDPSFAPA
                     VDNLVDYAITERITVEKILLTHGHGDHIAGIDKVHGAFPVAQVGIHPSDRPMLADGRR
                     NLSAMFGIDLSVETPADLELIVGMTLLLGNLSWQVLDTSGHSPGGVSFYCAQAGVVFT
                     GDALFPGSIGRTDFPGSNGRQLLANIRRNLLTLPDETVVYAGHGPPTTIGIERKSNPF
                     LAE"
     gene            6187..6591
                     /locus_tag="P5316_16150"
     CDS             6187..6591
                     /locus_tag="P5316_16150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRR86543.1"
                     /translation="MPGHEGKIETRCPACGARYRVPASGIGHRARCAKCGTSFRVAQL
                     APRRRPGDLHHPPTEDDILRWLNEGSDDEFLAPRPRIISGSDPSHPESHEETPVERPR
                     SVPTEPRVPLSGDVGNVIDSAPTGGGSLRKTG"
     gene            6988..8184
                     /locus_tag="P5316_16155"
     CDS             6988..8184
                     /locus_tag="P5316_16155"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRR86544.1"
                     /translation="MMVTRRWIYLGLTFMVAGIFGCPKPPVEIKPEPPDYWKKLPPGE
                     MALVKITDPAQYPDFSEAYYHQTNLEYAAANSLEYLSKESSKRYFPYLDITHDRAIRS
                     VQTLMDTLREAKSPEELDRLIKERFDIYVSRGCDDKGTVLFTSYYRPIFDARLKPDAE
                     FKYPLYKRPPDLELNEATMTYQRKGGGPYLTRPEIEHGALAGQGLELCYLRDRFEAYI
                     VTVQGSGKLRLEDGSFLEIGYAGDNGHEYTSVGQRLVDRGMIPPKELSLQGLIRYFKE
                     HPEELDSSLAVNKRYVFFTPRQGGPFGSLNVPVSPYRSLATDKQVYPRACPAYIQTVL
                     PARLNGVGEIADHRYKAFAFDQDTGGAIRAAGRGDLFIGTGPEIGELAGRTFSEGKLY
                     YIAVKE"
     gene            9165..10472
                     /locus_tag="P5316_16160"
     CDS             9165..10472
                     /locus_tag="P5316_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRR86545.1"
                     /translation="MTASRRRKSIPGRVIIVTSLLPLCIWGCALCNIDYRPLGQWKIE
                     PVQSARVVDGHPGRSEEFAAQLQLPHNSPRERSALSIKARHELTSQLGSHDYRLIGII
                     AGRGNAYTSLPELKQKMARRAAREGGDVLLITKTDVSQRPFVYTAPGNAYTNVYGNTA
                     HTTYYPGATYAGTLSFPSAMGWVLKHSPGWDEVAQKILLMDDATVAEIDAEWSAAASE
                     KLPYAEFEERMRSLIEAKAARFELAAATHPGPAGSRPASDGVVATAARSVVFIEADEA
                     IGSGFCIDTPRTILTANHVVEGASLILIRTPEGQKASAVVLRFDSDTDLAVLILSKSI
                     GLQPLRLRDSEPSLGDECYALGAPRGLTGTVTKGIVSAVRAGKLAQWVQTDAAINPGN
                     SGGPLIDRDGLVIGVCDWKLSGGGTEGLGFAVGAESIRKILNP"
BASE COUNT         2011 a         3283 c         3321 g         2030 t
ORIGIN      
        1 tcggcaggtc tccgccgcct ttggcggctt cgaccatgcc ctaccttttg ggtttgcctt
       61 cctgcttgcc cgcgatcttc gcccaggtgt ccttgagggt caccgtgcgg ttgaagaccg
      121 gcttgccggg cgttgaatcc ggatcaacgc agaagtagcc cattcgctcg aactggaatc
      181 ggctgccggg ggcggcgttg gccaaactcg gttcgacctt gcagccggtg agcacttcca
      241 gcgagttggg gttcaggccg gtcttccagt cctgtccctc gggaacgtcg tcgggatcgg
      301 gtttgctgaa caggtggtcg tacaaccgca cctcggcgtc gacggcttgg ccggccgaga
      361 cccagtgaat cgtccccttg accttacggc cgtcgggcga gtcgccgccg cgggtggccg
      421 ggtcgtaggt gcagtggact tcgctgacct cgcccgtggc cgggtccttc acgcagccgg
      481 tacacttgac gaagtaggcg tagcggagcc gcacctcctg accgggggtc agtcggaagt
      541 acttcggcgg cgggacctcg cggaagtcgt cctgctcgat gtacagcacc ttcgagaacg
      601 gcacgtgccg ggtcccggcg ccggggtcct cggggttgtt gatcgcgtcg agctcctcga
      661 cctgcccgtc aggataattg tcgatgacca ccttcagcgg ccggagcacg cccatgaccc
      721 gtggggctcg tttgttgagg tcctcccgga tacagtgctc aagaagctgg atgtccacaa
      781 tactgtcgta cttgttcacc ccgatccgcc ggcagaagtt ccagatcgct tccggcgtgt
      841 agccccggcg ccgcaagccg cagatcgtcg gcatacgcgg atcgtcccaa ccacgcacga
      901 gcccttcctt gaccagttgc agcagcttgc gcttgctcat caccgtgtag gtcagattga
      961 gccgggcaaa ctcgatctgc tgcgggtggt aaatgcccag ctggtcgaga aaccagtcgt
     1021 acagcgggcg gtggttctcg aactccagcg tgcagatcga gtgcgtgact tgctcgatcg
     1081 agtcttccag cccatgggcc cagtcgtaca tcggatagac gcaccactcg tcgccggtgt
     1141 gcgggtgagt cgcgtggagg atgcggtaca tgaccggatc gcgcatgttg aagttcggcg
     1201 aggccatgtc gatcttggcc cgcagcgtcc gcgaaccgtc ggggaactcg ccctttttca
     1261 tccgctcgaa aagatcgaga ttctcctcga cgctgcgatt gcgataaggg ctctcctggc
     1321 ccggttcggt cgccgtgccg cgcatggcgg caacctggtc ggcgctcagg tcgcaaacgt
     1381 aggccttgcc cgccttgata agctggactg cccactcgta catctgctga aagtaatccg
     1441 acgcaaagaa caatcggtct tcccagtcgg caccgagcca gcggacgtcc tcgatgatcg
     1501 agtcgatgta ctcctgctcc tccttgaccg ggttggtgtc gtcgaagcgc aggttgaact
     1561 tgccgccgta gtcgcgggcc agcccatagt tcaaacagat gctcttggca tgcccgatgt
     1621 gcaggtagcc gttcggctcc ggcgggaagc gggtcactac ccggccgccc cagcggttcg
     1681 tgcgcatgtc ctcatcaata atgtcgtgaa taaaggtccg gaataattca ccttcggtcg
     1741 tggccatgtc ctggtattcc ttcgaaaaag gcgagcagga tccgacgccg accccgcatc
     1801 gccgggcagg tttggcatta tacctctcct tgatccgtga tccatatccg ggctccggca
     1861 cgccgcaccc cgtgccgggt gacatcccca cggctttgcg tgggcatgcg ccttggcctc
     1921 ttccgtcgca actggtgcgc gttttgcgcg catgtttctg gatcgcagag cgcttgggtt
     1981 gtggtccgca aaaaggcggc cggccttatt gatctctgcg caggtactgc aagcatcagt
     2041 gtttgggaaa cccctgaaaa agggccgttc tggagaagag acgcgttgca ttctttgcgc
     2101 agaaatcaaa ggttggcggt gaacgcgaac gggaaacacg ccgcttcagc ggtctttccc
     2161 gaagggcaga taggccgatt ctgctgccgc ccccgttgcc cgatcctcgg tccccctgtc
     2221 tcgctcctcg taagagggca ccgaggaggt tacactgata cccggctgca ggcgcaacga
     2281 atgttcaact tcatagcgaa gaatggagac aaacatgcaa atcaggaatc gacgagactg
     2341 gctggtggcg gcgaccctct tgggtctggt ggcaaccggt ggatgtggga tcagcccggc
     2401 tatcaagcgg gccggccgca ccgacgagca gaagcagcaa ctgattaagc cgggcatgtt
     2461 cggcgacaac gcccccgcag atcgactccg cgggcggttt gacttttgga acgaggaggt
     2521 catccgccag gacgtggccg tcggcacggt gttcatgggc gactcgataa ccgaactgtg
     2581 ggaactggct gcctactttg caccgagcga cggggtcatc ctcaatcgcg gcatcggcgg
     2641 ggacatcacg ccgcatatgg cccgacgctt cgaggcggac gtcatccagc tcaaaccgcg
     2701 gaacgtcgtg attctggccg gcacaaacga cgttgcccgt atgctgagcg cgaagaagac
     2761 cgaagatgag acaatgtccc aggtgatgac caacatggag gcgatggtgg acgccgctca
     2821 aaaagcgggc atcaacgtct ttatctgctc gatccttccg accaacccgg acaccggcaa
     2881 ccatgcgggc aaggccccga tcctgcccaa gatcaacgag aaactgaaag ccatgtgtgc
     2941 cgccaaacat tgcatctatg ttgattacgc cgcgcacatg agcaactcgg ccggcgagtt
     3001 gcccagggat ctggcccgcg acggcctgca tccgcactac gccggctacg ccatcatggc
     3061 tcgcgtgctc aacgatgccg cccgggccaa cggcctccga ctgtaggaat aggacaggtc
     3121 tcggtcgccg acggcaacct cgacgctgcc agagcctgcc gcaacctggc tgccgccggc
     3181 ccgtcggggt cggtcagggc ttggttctca gcagcacgat cttcgcatcg atgtcggtcg
     3241 ggatcggcat catcactgcg tcccaggtcg ttggaatggg aatgatgact gcctgagacg
     3301 gcttgatgat gaccgggccg ggttgcacct ttttcacaat gatcggtgga accgccgatc
     3361 gcatcgcggg aggccgatat gcgaattgcg gccttgcagc catggccgtg gaactgagcc
     3421 cggtgattgc ggcaagcccc agcagagtgc caagccgaac gaggtgactg gtcatgatag
     3481 cacctcctga cctcattata tcagacgcac ggaggcggag aaggttccca cagggtcccc
     3541 aaagcgtgac gaaacccgcc acgccgacgc gtagagccgg ccccactcgg ggtgccgacg
     3601 ccctcgtcgg cactcgctca gactgagcat tcctcttcgc gcgagtaggg tacggcacgc
     3661 gacgccgctt agatcaggag aagacaaccg aattgaagcc gatcggcgag cctgtagaat
     3721 gaactgcggc tggtacatgg agatacccgt tttgaagctg ctgagcagag ggtcgcttcg
     3781 gtctctggtt gccgagggtc ttgtattggt tgcggtactt gctgctcgtc agcctccggc
     3841 catgggtaag accagacccg ctgagagcgc gtgtgggcaa gccagggcat ggtcggagcc
     3901 gcaggagacg gcggagacct gccgcccgcg actcagctat gctgcaggtc tcggtcgccg
     3961 tggcgacctc gacgctgccc taccgtctgg tcctgttggt tttcgtccag gctctgagcc
     4021 gacggagacc tccttgcctg ctactgcctc gaagccggcc agtggtcccg ctccgctcct
     4081 ttcgtctccc gcgatcgaac ggatcccgtt gccggttcaa ggcaagagcg gccccgcgtt
     4141 ctacgtgttg cgcaagcccg ccggttacgg ttcgggccaa ctgccgccgc tgattgttgc
     4201 attgcacggc accgacgaca ccgcggaaca gacggtcggg ttctgggccg ctcgcagcat
     4261 gcggattccc ccgttgatta cggcaccaca gggcgtcggt cccggatggc gaaacagtga
     4321 tacggccgtc attcacgcca tgttcaagca cttgcgggca aatgtcagct acgacgcgca
     4381 gcgagtgctg ttaacgggtt tctcggccgg cggggcaatg agttttgagc ttctttatgt
     4441 cgaggaggtt cccgtcacgg ccgtggccgc gctggccaac tatgtccccc cctggatcac
     4501 cggcgagcag gtcaaggcca aggcgcacgt accggtgttc tacgcggtcg gcatcgcgga
     4561 catcaaccac gagcaaatgc gcgagggtct ccaacggctt cgaaccgccg gtgccaggat
     4621 cgatctggat cgccctcgca tcggccacgt gctcgacagc gacgtggccc aggaagcgat
     4681 tgactggttt ttcgatcgct gtgccgatca agtgggcgct gctcttgacc ggatggccgc
     4741 tgcccgcctc gtcgccgacc tgccggcgct cgatcgaatc tgcctgcaaa gacgttggca
     4801 cgaaccgttg cacgtgcgtc gggcggagca gttgatcaag gaaatcgagg ctcccggaag
     4861 gctcgaactg gcgagagccc gggcattcat ggccgagggg cgggcggccg atgccgtgga
     4921 ggtcctgcag gaaatcgagg gccggttcgt cgccggaccg cttgcgcgcg aggccaaggc
     4981 cctgcgtctg cacctggagt ccgaccccgc cgttcgccgc gaaattgccg accgccttgc
     5041 cggccgccgg gctgagcagg cactgtccat gtacgtgaac gcccagcggc tggtcgtgca
     5101 gcgtcagttt gacgaggcag cccggcagtg ccgccggatc atcgatcttt acggcgaaac
     5161 gcccggcgcc gagcgggcca ggacgcttct tcaacttctg gagaacagga aaaagcagtg
     5221 agccgcaacg accttcgcat taaggccttt gtcgatccga gtttcgccga gaacgcctac
     5281 gtcgtcagtg tctcatccga tgatggaaat cgcgtcggtt gggtgatcga tccgagtttt
     5341 gcacccgccg tcgacaacct cgtggattac gcgatcaccg agcgtatcac cgtggagaag
     5401 atccttctga cccacgggca tggggaccac atcgccggca tcgataaggt tcacggcgcg
     5461 tttcccgtgg cccaggtggg tattcacccg tcggacaggc cgatgctcgc cgacggacgc
     5521 cgaaacctgt ccgccatgtt cggaatcgat ttgagcgtcg agacccccgc cgacctggag
     5581 ctgatcgtcg gaatgacctt gctgcttggg aacctttcct ggcaggtctt ggacacctcg
     5641 gggcattcac ccggcggggt ctcattctac tgtgcccagg cgggcgtggt gtttacgggc
     5701 gatgccctgt ttccgggcag tattgggcgg acggatttcc cgggctcgaa cggtcggcaa
     5761 ctgcttgcca acattcgccg caacctcctg accctgccgg atgaaaccgt tgtttacgcc
     5821 ggacacgggc ccccgaccac catcgggatt gaacgaaagt cgaacccctt cctggccgaa
     5881 tgattggttg ggcgcttcca gcgcggcgca gcggtccgcg ctgttttcgt cgggatggtg
     5941 ccgcacgtcc gtggccgaat cccgccggaa aacgcgttga aatctgcggc gctgcgggca
     6001 tagaattgag tgaaggcacc gcgaagaggg gccaccgcct gagggtggtg gagccagcca
     6061 gatacttctc aaagttgtca taacgcaggt gccgcactcg atgcccggag tatcagcgcc
     6121 gggtggtcgg cgacgtgcgg ctcgatacgt cagaccgccg ggaaggaggc tctcggagga
     6181 ccggccatgc caggccatga gggcaagatc gagacccgat gccccgcctg cggcgcgagg
     6241 taccgcgtgc ctgcctccgg catcggccac cgggcccgct gtgccaagtg cggcacctca
     6301 tttcgcgtgg cccagctcgc ccccagacgc aggcctggcg atcttcacca tccgcccacg
     6361 gaagatgaca ttcttcgttg gctcaacgaa ggttcagacg acgagttcct ggctccccgg
     6421 cctcggatca tcagtggcag tgacccgagt catcccgagt ctcacgaaga gacgcctgtc
     6481 gaacgtccac gctcagtgcc gacggagccg cgcgttcctc tgtcgggcga tgtcggcaat
     6541 gtcattgact ccgcgccgac tggcggcggt tcacttcgca agacaggttg acaactccag
     6601 gggacgcctc ggccgattct tgtcaccatt ctggcacgag ctgctgatgg caactgaagc
     6661 gattggcatc ctgtccgcat tgcctgtcgg aaattcgccg tcctgacagc ggggcatcgt
     6721 ctgtgtggcg aataaccgtc gacagagctc ctcgtccaac cgcacaaggg gccgtcggat
     6781 cggttgccgg caatccgtac gcgtttcttg ctccgattgt cacgcaagtt cgttgctgaa
     6841 ggccgttcaa accaccgata atggtgatgt tcgccggggg tgggatcaag gggcgagagc
     6901 acaatcgtgg atcagtagtg tgtcatggtt gccggccggc ggccgtcgcg tcgcaggcag
     6961 gcaggattgg ccagggagga cgtttctatg atggtcacgc gtcgctggat ctatctcggt
     7021 ctcacgttca tggtggcggg gatcttcggc tgtccaaagc cgccggtgga gatcaaaccg
     7081 gagccgcctg attattggaa gaaattgcct ccaggcgaga tggccttggt taaaattact
     7141 gatccggcac agtacccgga cttcagcgag gcctactacc accagaccaa tctcgagtac
     7201 gcggcggcca acagcctgga gtacttaagc aaggagtcgt cgaagaggta cttcccatac
     7261 ctggatatca cacatgatcg ggcgatccgc agcgtccaga cgctcatgga tacgctccgg
     7321 gaggccaagt cgcccgaaga actcgaccgt ctgatcaagg agcggttcga catctacgta
     7381 tcgcgtggat gcgacgacaa gggtacggta ctttttacca gctactatcg cccgatcttc
     7441 gacgctcgcc tgaagcccga cgcggagttc aagtacccct tgtacaagag gccacccgac
     7501 cttgagttga atgaggccac gatgacctat caacgcaagg gcggcggtcc gtacctgacc
     7561 cgcccggaga tcgagcacgg cgcactggct ggtcaggggt tggaactgtg ttacctgcga
     7621 gaccgtttcg aggcgtacat cgtcaccgta cagggatccg ggaaactccg cctcgaggac
     7681 ggctcgttcc tggaaatcgg ctacgccggc gacaacggcc atgaatacac ctccgtcggc
     7741 cagcgactgg tcgatcgggg catgatcccg ccgaaggagt tgtctctgca gggtctcatc
     7801 cggtatttca aggagcatcc ggaggagctg gattcaagct tggcggtcaa caaacggtac
     7861 gtgttcttta ccccgcgcca gggcggcccc tttggcagcc tgaacgtgcc ggtttcgccg
     7921 tatcgttcgc ttgccaccga caagcaggtc tatccgcggg cctgcccggc gtatattcag
     7981 acggttctcc cggcccgcct caacggcgtg ggcgagattg ccgaccaccg ctacaaggcc
     8041 ttcgcgtttg accaggacac cggcggtgcg atccgggcgg ccggtcgcgg cgacctgttc
     8101 atcggcaccg gccccgagat cggcgaactc gccggacgta ctttctctga aggcaaactc
     8161 tactacatcg cagtgaagga ataacggtca aacccccgcg cgtcaccgtg cggacaacac
     8221 cacccaaacg gccgggtcca gcatgctggt ccggccgttt tcttctgtac gttacccgct
     8281 gacgctgtat cgcggggaac cgccgagagg agactgtacg ggtaccaccg catcggcaac
     8341 cggcagaacg tgacgatcgg ggactataca accccgacct tgcgctactg tgccgatgac
     8401 ctgaaccgct gcaaccagac caacacccgc aacccgcaga cctgcccggt aacctcgacg
     8461 agcgagtcgt tcatctgcga tgaggtcggc aaccacaaga ccgacggcct cttcaagtgt
     8521 acctgggacg ccggaaccct tgacgcgacg gccaacgcca tgcgttggag cctccggatt
     8581 gcggcaccgc cgcccgtcgt gtcaagggag aaccgcccga ttgcggtcac gccgcggacg
     8641 ctggcccccc cggtcttgac gagccgaggg gtttatcccc tcggtcttga catcttgacg
     8701 agccgagggg tttaccccct cggtcttgac atcttgacga gccgaggggt ttatcccctc
     8761 ggtcctgacg tcttgacgag ccgaggggtt tatcccctcg gtggtttatc ccctcggtct
     8821 tgggcccgga aaagggttca gaagccattt tcagtaaagg tggtaacagg agggcacatt
     8881 tctcagcgta ctcagcggtt gacaagagcg atcagccttc agcggccgga gaaacagtgt
     8941 ttcaccgcag tggacgcgga gggacgcgga caacattcac gttcacttcc agaaacgtgc
     9001 ccataacccc tacctcatcg ccgccggatt cgccaagacc gagctcccca tcctctggct
     9061 cgccaacaag cgactgggct ttacgttcgg ctgaaaagcg gcaaaatgtt agtcggcaga
     9121 aaagctatgg gaatcaaggc tggacaagaa aggcggacat cagcatgaca gcttcgcgaa
     9181 ggcggaaatc tatcccgggc cgtgtgatta tcgtcaccag tctgctgccc ctgtgtatat
     9241 ggggctgcgc cctgtgcaat atcgactaca ggccgctggg gcagtggaag atcgagcctg
     9301 tccaaagtgc aagggtggtg gatggtcatc ctggccgaag tgaggagttc gccgcccagt
     9361 tgcagttgcc ccacaacagc cctcgggaga ggtcagcatt gagcatcaag gccaggcacg
     9421 aactgacctc tcagcttggc tcgcacgact atcgtcttat cggcatcata gctggccggg
     9481 gcaatgccta cacctcgctg ccagagttga agcagaagat ggcgcggcgc gcagccaggg
     9541 aaggcggcga tgtgctcttg atcaccaaga cagacgtgag tcagcgacca tttgtttata
     9601 ccgcgcccgg caacgcttac accaacgtgt acggaaacac tgcacatacg acgtactacc
     9661 caggtgcgac gtacgctggc acgctttcct ttccctcggc gatgggctgg gtactgaagc
     9721 actcgcccgg ttgggatgaa gtcgcccaga agattctgtt gatggatgac gctacggtgg
     9781 cagaaatcga tgcggaatgg tctgccgccg cctcggagaa gctgccctat gcggaattcg
     9841 aggagcgaat gcgctcgctg atcgaggcga aggcggccag atttgaactc gcagcggcga
     9901 ctcacccagg ccccgccggt tcacgcccgg cttccgatgg agtcgtggcg acagcggcac
     9961 ggtcggtcgt gttcatcgag gcagatgagg ccatcggctc ggggttctgc atcgatactc
    10021 ccaggaccat cctgacggcg aatcacgtag tcgagggggc cagcctgatc ttgatacgaa
    10081 cgcccgaagg gcagaaagcc tctgccgtgg tgctgcgatt cgattcggac acagaccttg
    10141 ctgtattgat cctgagcaaa tccatcggat tgcagccact tcggctacga gactccgaac
    10201 cgagcctcgg cgatgaatgc tatgcattag gggcacccag gggcctgacc ggtactgtga
    10261 ccaaggggat cgtcagcgct gtccgggcgg gcaagctggc ccagtgggtc cagaccgacg
    10321 cggccatcaa ccccggcaac agcggtgggc cgctgatcga ccgcgacggc ctcgtcatcg
    10381 gagtctgtga ctggaagctc tccggcggcg gcaccgaggg tctgggcttc gccgtcggcg
    10441 ccgaaagcat ccgcaagatc ctgaacccgt gaccagggcg tcgcaacggg gacccagcta
    10501 gtttttgaga agcggtcagc gatcggccgt cagcaatcag cacttcaccg cagtggacgc
    10561 agaggggcgc ggagacgaag gtgagcatga gtcttgtttt tgcgcttttc gcgcttcttg
    10621 tggctgcact tcggtctggc cgagg
//
LOCUS       DAOTPW010000200        10636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5838_length_10636_cov_5.181552, whole genome shotgun sequence.
ACCESSION   DAOTPW010000200 DAOTPW010000000
VERSION     DAOTPW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_10636_cov_5.181552"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1378)
                     /locus_tag="P5316_16165"
     CDS             complement(287..1378)
                     /locus_tag="P5316_16165"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRR86546.1"
                     /translation="MAAKPRIHLTAVASYATQGMQLLGIRNVGELIALASSRLNGRYA
                     VTAGGPMIFAKQDEMHGGRFDDAARAREIETILADDDVAALVTVRGGSWFTRILQKID
                     FDVLKRRRTTIHVFGFSEMTSLVLIAGRYPKVFALHDLGPLFLFDGVRRFMTKRADAY
                     LQAAEMPVDAAHCAGFGAGWAAAKFRGMFADFIAEVAAILDGHGSPRVPSGRLLEGKL
                     PAAKPITIVGGNISVIMPLIGSRYAEFIDPTGKWLALEEINESVGAVDRMLAGLKLSG
                     LLDKAEGIILGNFRDGDANLTQAVFEALKRHQRTSRNQPVIELTNFGHVYPIAPLPMH
                     RQVILRCRRAARGQTQVSIETPWDKWGRE"
     gene            complement(1383..2204)
                     /locus_tag="P5316_16170"
     CDS             complement(1383..2204)
                     /locus_tag="P5316_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HRR86547.1"
                     /translation="MDGPIRRLPDQAIIGMVHLAPLPGSPAGRQPLSKVVQAACHDAR
                     LLADAGFDALIIENFGDAPFRPTTVDPHTVAFMTVAVRAVMAAVSIPVGVNVLRNDAI
                     AAVAIAGVCGAAFVRVNVHTGAYATDQGLMEGRADETLRYRRRLTGDAQGNQTAAMPA
                     ILADVHVKHAMPLAAAPIAQTAEEAAYRGRADGLIVSGAATGKPTDLADLKAVREAVP
                     DRPVYVGSGVTPDTVAEWLAVANGVIVGTAIKRDGLTTAPVDAARAAALVKAARR"
     gene            complement(2230..2550)
                     /locus_tag="P5316_16175"
     CDS             complement(2230..2550)
                     /locus_tag="P5316_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRR86548.1"
                     /translation="MREIRPQKTRLETVEPLGKRVLVRKDEDRKKTKGGIALPDNIEI
                     PTITGRVVTVSQMLSNDNDFPVRQYDRVLFNPKNSIPVDFEPGNQLYVVPVDDIVAVF
                     RDRR"
     gene            complement(2711..3124)
                     /locus_tag="P5316_16180"
     CDS             complement(2711..3124)
                     /locus_tag="P5316_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86549.1"
                     /translation="MPATGCQQGYRWDLGDVVKAEQRARDEGKILFVFYQLWTDPTSN
                     RMKGRELLSDPEVEAEFRDTINVLVDRDFGSMYVGSLRKYQVTTYPAFILVSPDGKYR
                     ALTGAIPRSQFLAWVRDFKSRTRQPASPAVKPGGK"
     gene            complement(3237..3887)
                     /locus_tag="P5316_16185"
     CDS             complement(3237..3887)
                     /locus_tag="P5316_16185"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR86550.1"
                     /translation="MEERTTRKIYNVWSYFYDLFWPSIVHRRTTRAIERMCIQPGDRV
                     LDIGVGTGLALASYPKHARVVGIDLSEGMLRRARKRIEEHRMSWVSLTLGNALQLPFE
                     DGVFDHVLLSHVVTVVSDPVKLIEETRRVSKPGGQVVIINHFRSSNRVFGTIEKWLCP
                     LCQHLGWRSDLCLQELAQATGLEVDFRYKLDNMDLFQTVFITNRPALRPERQSTAA"
     gene            complement(4676..5275)
                     /locus_tag="P5316_16190"
     CDS             complement(4676..5275)
                     /locus_tag="P5316_16190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR86551.1"
                     /translation="MKGKWMALTLAALGAGVCLYGYSLAAEPGKAAEPGQKAPDFALK
                     DVYGKEFKLADFKGRIVVLEWINKGCPISRAAHDKQQMQKTYKKYAAKDVVWLGIDST
                     EGTKPDENRVYAAEQSLAYPILHDVDAKVANAYGAKRTPHMFVIDKEGKLVYAGAIDD
                     KGDNNYVAAAIEDLLAGKPVAKPKTDAYGCGIKNSKLAS"
     gene            5592..6365
                     /locus_tag="P5316_16195"
     CDS             5592..6365
                     /locus_tag="P5316_16195"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRR86552.1"
                     /translation="MPSKDLRPIIQDWPFESGQIKVRKIRGLDNRVKIQMRVDLGVLQ
                     METEGRPDGQRPFNYESLLEYHLARLESHKRRNGTDLGFSLSPDECLAIRDESLQYYH
                     RYLASFAMEDYDMVVRDTQRNLDVLDLCCKYGEQESDRMALEAHRPYIIMMNTRAKAL
                     GAIGNGQYLTALAHVERGLVSIRDFFKKYGTSKSFNHSPEVVILKALRREVRKQLPVD
                     PIRKLKQELADAVAEERFEDAAQLRDSLEALLKQHGGQS"
     gene            complement(6453..7808)
                     /locus_tag="P5316_16200"
     CDS             complement(6453..7808)
                     /locus_tag="P5316_16200"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRR86553.1"
                     /translation="MDVREEQMPRGFGLATTTFVVIASMVGTGILVSPGYMMASLGNH
                     TVIFGLWILGGLLALCGALCVAELAAAMPRAGGEYVYLREAYGPMPAFLSGWTSFFLG
                     FSAPLAVAAHVASKYLLQASGMVAAGSAASLPTKVLATAIIILVTAPNLLGHRQSAWV
                     QNLTTVVKLGVFVAAVAAGFICGEATFQQIAGNRSPANVGLGTAASQLFYVMFAYSGW
                     NAASYLAGEVKNPAGMLPRSLLLGTAAVTALYLAMMLFFAASVPLVKVGFHNAEKVPW
                     MAVEQSLGRRAAGAFSAVVGLTFLATVNAFIVTGPRVYFAMACDGLFPSIAGRISPRG
                     RVPVNATLAQSACAIAILFLTDFESLYQYAAVGLSLFALLFIAAVYVLRIRRPQMPRP
                     FRVPGYPVVPAFFMTATLFTAVFAFKEWPMPSLLSLGSILAGVPVYYAWRKLRRRTAK
                     T"
     gene            complement(7916..8752)
                     /locus_tag="P5316_16205"
     CDS             complement(7916..8752)
                     /locus_tag="P5316_16205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR86554.1"
                     /translation="MPPACMVCSHQGFEPFLDILLKCPVCGFVTARLENPIDARQIYQ
                     GAYFTGQEYLDYLGDEALLKRNFRRRLKHIRKRCPGGRLLEIGSAYGFFLDLARAHFQ
                     TVGFEINWEAARHAREVLGLDVRTDDFLLADEHTIGGKVDVTVMWDVIEHLERPDRFL
                     ARVAELSRSGAILCLTTGDVGSVLARWRGRKWRMIHPPSHLHYFDRNTITRLLGNHGF
                     EVIDIRAIGVARSLRQILYSLLVLGLRLPVAYRVLESVIPAAWGVTLNTFDIMQVTAS
                     RR"
     gene            complement(8779..9747)
                     /locus_tag="P5316_16210"
     CDS             complement(8779..9747)
                     /locus_tag="P5316_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86555.1"
                     /translation="MFTKFLGIATNSLTETVRQPIYGILLLASTFVLVLNLGLAGYTF
                     DDDNKLLKDLSLSTMLVCGLFLAAFSAAGVLSREIENKTVLTVVSKPVGRPVFILGKF
                     AGLSGALAIAYYLNGIVFLLTMRHKVMSRSSDHFDVPVIVFGCAAFFTTLLIAALCNY
                     LYDMQFSSTSVTISIPLMTLALVLVCLIGPTWEIQQFGKDFIDGQIIGAVALVFAMVM
                     IATAVAVAVSTRLGQVATLAICTLVLMIGLASDYLFGQLRDTSLLARFAYWLSPNLAF
                     LWVADALTQNNRITAPYVGMAFGYAMLIVLAWLLIAVALFQKREVG"
     gene            9977..>10636
                     /locus_tag="P5316_16215"
     CDS             9977..>10636
                     /locus_tag="P5316_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86556.1"
                     /translation="MTTQRPERRARTVALAGLAFQILLSVFFAVLWSWGKTEGLRALM
                     LLSAIGNAIWLFLVMVCQQRVLVQEEKFETEQLRRERAAAGGTDAIFDVADEELLLAR
                     RRLQWMYRWMLPSFSILVIVLLVVAGLVGWRWSIGDSFSSENWTAVGKDKIPLLFFMT
                     LGAAFVSFLFSRYATGMARYREWQILHAGATWLMGVTLASVLVSAAMGALYMGATPVF
                     ER"
BASE COUNT         2203 a         3083 c         3365 g         1985 t
ORIGIN      
        1 gaaaggattc cgggccgagc ggtttggagg tgtggtcgga catgagcggt caaaatggcg
       61 cgcagagcat gccctaccgt ggtgacatct caatggttgg tggcgggtcc ggcgacctgc
      121 cgtgcgggaa tacgcttgcc tgggggcaag gatgggccga cgggggcgtc ggccctacag
      181 acgctcgaac tcagcctacg ctggatgcag agatgggccg acgggggcgt cggccctacc
      241 ctccatgcct acgcatggcc gtggggatgg cgcccggcac gggttgttac tctctccccc
      301 acttgtccca cggagtctcg atgctcacct gggtctggcc gcgggccgcg cggcggcaac
      361 ggaggatgac ttggcggtgc atcgggaggg gggcgatcgg gtagacgtgt ccgaagttgg
      421 tcagttcgat gaccggttgg ttacgtgagg ttcgctggtg acgcttgagc gcctcgaaga
      481 ctgcctgcgt caggttggcg tcgccgtcgc ggaagttgcc gaggataatt ccctcggcct
      541 tgtccaacag accggacagt ttgaggcctg cgagcatgcg gtcgacggcc ccaaccgatt
      601 cgttaatctc ttcaagggcg agccacttgc cggtgggatc gatgaattcg gcgtagcgtg
      661 agccgatcag gggcatgatc acgctgatgt tgccgccgac gatggtgatg ggcttggcgg
      721 ccggcaactt gccctccagc aaacgtcctg agggcacacg cggtgagccg tgcccgtcaa
      781 ggatggccgc cacctcggcg ataaaatcgg cgaacattcc ccggaacttg gctgcggccc
      841 agccggcgcc aaaacccgcg caatgggcgg catcgacggg catttccgcg gcttggaggt
      901 acgcatcggc ccgtttggtc atgaaacggc gcaccccgtc gaacaggaac aacggcccaa
      961 gatcgtgcag tgcaaacacc ttcggatacc ttcctgcaat gaggaccaga ctggtcattt
     1021 cgctgaaccc gaagacgtgg atcgtggtgc ggcgtcgctt gagcacgtcg aaatcgatct
     1081 tctggaggat tcgcgtgaac catgacccgc cgcgcacggt gacgagcgca gcgacatcgt
     1141 cgtccgccag gatggtttcg atctcgcggg ctcgagcggc atcgtcgaat cgcccgccgt
     1201 gcatctcgtc ctgcttggcg aagatcatcg gcccgccggc cgtgacggcg taacggccgt
     1261 tgagtctgct tgatgccaag gcaatcagtt caccgacgtt tcggatgccg agcaactgca
     1321 tgccctgggt ggcgtagctg gcaacagcgg tcaggtgaat gcgaggtttg gctgccataa
     1381 tttcacctcc ttgccgcctt aaccaaagcc gcagcacgcg ccgcgtcgac gggggcggtg
     1441 gtcaggccgt cccgcttgat cgccgtaccg acgattacgc cgttggcgac ggcgagccat
     1501 tcggccacgg tatcgggcgt gacgccgctg cccacgtaga ccggccgatc cggaaccgcc
     1561 tctcgaacag ccttgaggtc ggccaggtcg gtcggctttc cggtggccgc gccgctgacg
     1621 atcagtccgt cggctcggcc ccggtacgcg gcttcttcgg cagtctgcgc gatcggcgcg
     1681 gccgccagcg gcatagcgtg tttcacgtgg acatcggcaa gaatcgcggg catcgcggcg
     1741 gtctgattgc cttgtgcgtc gccggtaaga cgacgccggt atcgaagcgt ctcgtcggcg
     1801 cgcccctcca tgagcccctg gtcggtggcg taggcgcccg tgtgcacgtt gacacggacg
     1861 aacgccgctc cgcagacgcc ggcgatggcc actgcggcaa tcgcatcgtt ccggagaacg
     1921 ttgaccccca ccgggatgga gaccgcggcc atcaccgctc gcacagccac ggtcatgaag
     1981 gcgacagtgt ggggatcaac ggtggtcggc cggaaggggg catcaccgaa gttctcaatg
     2041 atcagggcgt cgaagccggc gtcggccaga agccgggcat cgtggcacgc cgcctggacc
     2101 accttgctca aaggctgccg gccggcggga cttccgggca atggagcaag atggaccatg
     2161 ccgataatcg cctggtccgg cagacgtctg atgggcccgt ccatgaatca gactcggagg
     2221 tggactgtct caacggcggt ctcgaaatac cgccacgata tcgtcaaccg gtacgacgta
     2281 tagctggttg ccgggctcga aatccacggg aatggagttt ttcgggttaa acagcacccg
     2341 gtcatactgt cggaccggaa aatcgttgtc attggaaagc atctgcgaga ccgttaccac
     2401 gcgcccggtg atcgtgggaa tctcgatgtt gtcgggcagg gcgattccgc ctttggtttt
     2461 cttgcggtct tcgtccttgc ggacgaggac tcgcttgccg agcggttcga cggtctcgag
     2521 tctggtcttt tggggtcgga tttcacgcat tacgccgtct cctcttgccg gagggcaaga
     2581 tacttgacca cgaaagcttc agcgatagcc atcacaggaa accaagtcaa ttatagagga
     2641 gatgcggccg gcgccaactc cggcaaggcg acaaacaacc acgacgacat ggcaggtcga
     2701 gaaaaggctg ttactttccg cccggcttga cggccgggga agcaggttgg cgtgtccgag
     2761 acttgaaatc gcggacccac gcaaggaatt ggcttcgggg aatcgcaccc gtcagcgccc
     2821 ggtactttcc gtcgggcgaa acgagaatga aagcgggata ggtagtcacc tgatatttgc
     2881 gcagggagcc cacgtacatc gatccgaaat cgcggtcaac caggacgttg atggtgtccc
     2941 tgaattcagc ctccacctcg gggtcggaaa gcaattcgcg gcccttcatc cggttggagg
     3001 tcgggtccgt ccagagttga tagaaaacga agaggatctt gccctcatcg cgggcccgct
     3061 gctcggcttt cacgacatcg ccgagatccc agcggtatcc ttgttgacaa ccggtagcag
     3121 gcatcagagc cgccaagcaa agcaacggga ttccccggca aggccgatcg gacagacaca
     3181 accgtttgcg gaaactggta tgcatgttca ccattatccc tgatgggtgg agcatctcac
     3241 gcggcagtag attgtcgttc cggccgtaag gcgggccggt tggtgataaa aacggtctgg
     3301 aacagatcca tgttgtccag tttgtagcgg aagtcgactt cgagccccgt cgcctgggcc
     3361 agttcctgca agcacaggtc gcttcgccaa ccaaggtgct gacagagcgg acacagccat
     3421 ttttcgatgg tgccgaacac ccggttgctc gatcggaagt ggttgatgat gaccacctgc
     3481 ccgcccggct tgcttacccg ccgagtctcc tcgatgagct tcaccggatc gcttaccacg
     3541 gtcacgacat ggctcagcag tacatgatcg aacacgccgt cctcgaaggg cagttgcagg
     3601 gcatttccca gagtcaggct gacccacgac atgcggtgct cttcaattcg cttgcgggct
     3661 ctgcgcagca tgccttcgct gagatcgatc cccaccaccc tggcgtgttt cgggtaggaa
     3721 gccaaggcca gaccggttcc cacgccgata tctaacactc ggtctcccgg ctggatgcac
     3781 atgcgctcaa tagcccgtgt cgtgcggcga tgcacgatcg acggccagaa aagatcataa
     3841 aagtaagacc atacgttgta gatcttacga gtggtgcgct cttccatcgg gccttacctc
     3901 gtgtcgttgc tcaatccaag gcggaccgcc cttttgcggg ccgcagccaa agcgcactac
     3961 gatcccgaaa catgcgcaaa atccacgccc gtcatgacgg aagagagtaa cgccgctatc
     4021 ggcgggccgc aagttgagcc ttacggccgg gccttgcttc acgtgaactg cgcccggcga
     4081 ccagacgcat gcgtcgagtt tgcctttggt ccctgaggcg aagacgcggc aagtgagcac
     4141 tggggggcag ttgatcggtc gagcgcccaa cgatggtctc gctggaccag cgggatctgt
     4201 gccgtgggcg ttgcaagttc cccgccaccc gccgagacct gaaaaaaagg cgacgaccag
     4261 ccgtcgccga tcgtcgctgt tctctctccc gtaagctagg gttacgcctc gttgaagatt
     4321 atacggcgcg caggtgtccg ggtcaaaaga agactctaaa ctatttggaa ttccagtact
     4381 tacaaaacct aacacaaacc caatctgctt gctcgccggc agaaaatcac agtgcccaac
     4441 tatgaccgaa gagagcttgt cggtacacaa aaggaagctt gcaggcaagc ggaaacggta
     4501 ttatcgggct gccagcttgg ctggcatgca aatccaatct tgtcttggtt ggtcggcgtc
     4561 acagcgattg tgtctttggg ccttctatca tggccaggtc aggagcgtgt attgcgctgc
     4621 ccgcaaggtg gggggccaaa gggggtacct gggcccagag gatcgctccg gcgtatcacg
     4681 aagccagttt ggagttcttg attccgcagc cgtaggcatc ggttttgggc ttggcgaccg
     4741 gcttaccggc gagcaggtcc tcgatggcag ccgcgacgta gttgttgtcg cccttgtcat
     4801 cgatagctcc ggcgtagacg agctttccct ctttatcgat cacgaacata tgtggagtcc
     4861 gtttggcccc ataggcgttg gcgaccttcg catcgacgtc gtgaagaatc ggataagcca
     4921 agctctgttc ggcggcgtag acccggttct cgtcgggctt ggttccttca gtcgagtcga
     4981 tcccgagcca gaccacgtcc ttggcagcgt acttcttata ggtcttctgc atctgctgct
     5041 tgtcgtgggc agcgcggctg atcggacaac ccttgttgat ccactccaaa acgacaatcc
     5101 tgcccttgaa gtcggcaagc ttgaattcct tgccatacac gtccttaagg gcaaaatcgg
     5161 gggccttctg gccgggttcg gcagctttgc ccggttctgc ggctaaggaa tagccataga
     5221 gacagacgcc cgcgcccaag gctgccagtg tcagcgccat ccattttcct ttcatgtccg
     5281 ttcctccctt attgacaggt ccgaaaccac ctgactgcat gctattactg ctgcttcaat
     5341 aagcacagtg cccaagtcaa tgccatattt gcccaagccg ctgcccggaa agcaccgatg
     5401 gtcctcggcc atgatgcaag ggacggctcg aaggggagtt tagtcctctg ccggtgggga
     5461 ggcaaccggc ctggttttgc gtcgcttgat atggagtcta aataccccaa cgcgtaagat
     5521 atatggtgga gctcgaggcg ggttgcgaat acccccgaag cccaagccgc cagcaaagca
     5581 tggagccgag gatgccatcg aaggatctgc gcccgatcat tcaggattgg ccctttgaat
     5641 cgggccagat caaggtccgc aagatccgcg ggctggataa ccgagtgaag atccagatgc
     5701 gcgtggatct gggggtgctc cagatggaga cggaaggccg gcccgacggg caacggcctt
     5761 tcaattacga atccctgctg gaataccatt tggcccggct tgagtctcac aagcggcgca
     5821 acggcacgga cttgggtttc agcctgtcgc ccgatgaatg tctggcgatc cgcgacgaga
     5881 gccttcaata ctaccatcgg tacctggcca gtttcgcaat ggaagactac gacatggtcg
     5941 tccgggacac ccagcggaac ctggacgttc tggacctgtg ctgcaagtat ggcgagcagg
     6001 aaagcgaccg gatggccctg gaggcacaca ggccgtacat catcatgatg aacacgcggg
     6061 ccaaggcgtt gggcgccatc ggcaacgggc agtatcttac cgctctggcg cacgtggagc
     6121 ggggattggt cagcattcgc gatttcttca aaaaatacgg aacttccaag tcgttcaacc
     6181 acagtcccga ggtcgtgatt ctcaaggcgt tgcggcgtga ggtccgcaag cagttgcccg
     6241 tggacccgat tcgcaagctc aagcaagagc ttgccgacgc tgtggcggaa gagcgatttg
     6301 aggacgccgc ccagctccgc gacagtctcg aggccttgct gaagcaacac ggcgggcagt
     6361 cgtaagcggg tgtgcgcggt cgagcgtcgg ggggtcctgg ggcgacggcc attaatcggg
     6421 ttcgtcgtgt caagccttct ccaagtgatc ggtcacgttt tggcggtccg acggcggagt
     6481 tttcgccacg catagtacac cggcacgcca gcgaggatgc tgccgaggct cagcagcgac
     6541 ggcatcggcc attccttgaa cgcgaaaacg gccgtgaaga gggtggcggt catgaaaaag
     6601 gccggcacga caggatatcc tgggacccga aacgggcggg gcatttgggg acggcggatg
     6661 cgcaacacat acactgcggc gatgaagagc agggcgaaca gcgagagtcc gacggcagcg
     6721 tactgataga ggctctcaaa atcggtcaga aagagaatcg cgatcgcaca ggcgctttgg
     6781 gccagcgtgg cattgaccgg gacccgccct cgtgggctga ttcgcccggc gattgacggg
     6841 aagagcccgt cgcaggccat cgcaaaatag acgcgcggtc cggtcacgat gaaggcgttc
     6901 accgtggcca ggaaggtcaa cccaaccacg gccgagaacg cgcccgcggc acgtcggccg
     6961 agcgattgtt cgacggccat ccatggaacc ttctctgcgt tatgaaaacc gacctttacc
     7021 agcgggaccg aggccgcaaa gaacagcatc atcgccagat acagtgcggt caccgccgcg
     7081 gttccgagca acagggaacg aggtagcatc ccagcggggt ttttcacctc gccggcgagg
     7141 tagcttgcgg cgttccaccc gctgtaagcg aacatcacgt agaacaactg cgatgcggcg
     7201 gtgccgagcc cgacattcgc cggggatcga ttgccggcga tctgctggaa agtcgcttct
     7261 ccgcaaatga acccggcggc aaccgcggcg acaaagacgc ccagcttgac gacggtggtg
     7321 aggttctgca cccaggctga ctggcgatgg cccaggagat tgggggcggt aaccaggatg
     7381 atgatcgcag tcgccaggac cttcgtgggc aacgacgcgg ccgacccggc cgcgaccatc
     7441 ccggaggcct gcaaaagata cttggaggcc acgtgggccg cgaccgcgag cggagccgag
     7501 aaaccaagga agaacgaggt ccaaccggat aaaaatgccg gcattggacc gtaggcctcg
     7561 cgcaggtaca cgtactcacc gcccgcgcga ggcatcgcag cagcaagttc ggccacgcac
     7621 aacgcaccgc acagcgcgag gaggccgccc aggatccaga gaccgaagat gaccgtgtga
     7681 ttgccgagcg aggccatcat gtagcctggt gaaacgagga ttcccgttcc gaccatgctg
     7741 gcgatgacca cgaaagtggt ggtggccaac ccgaaaccgc gaggcatctg ctcttcacga
     7801 acgtccatgg gtcactccgt ccgggcgtca tcatcgcgac tttgggacga aacaccagcc
     7861 cccggcgttt gcgcagcggt ggtgtggcga tcggggccgg cgcgtcgatc tcttctcagc
     7921 ggcgcgacgc ggtgacctgc atgatgtcga aggtattgag ggtgacgccc catgcggccg
     7981 ggatgacact ttccaagacg cggtacgcga ccggcaagcg gagaccgaga accagcagcg
     8041 agtagagaat ctggcgaaga ctgcgggcca caccgatcgc ccggatgtcg atgacctcga
     8101 acccgtgatt gcctaacaga cgggtaatgg tgttgcgatc gaagtagtgc aggtgcgatg
     8161 gcgggtgaat catccgccac ttgcggccgc gccagcgggc caagacgctg ccgacgtcgc
     8221 cggtagtgag acacaggatt gctccggacc tggacagctc ggcgacgcgg gcgaggaagc
     8281 gatcaggacg ttcgagatgc tcgataacgt cccacatcac ggtgacatcg accttgccgc
     8341 cgatggtgtg ctcatcggcc agcaggaaat catccgttcg cacgtcgaga cccagcacct
     8401 cccgggcgtg gcgggccgcc tcccaattga tctcgaaacc taccgtctgg aaatgtgccc
     8461 gggcaaggtc gaggaaaaag ccgtaagccg acccgatctc aagcaggcgc ccgccgggac
     8521 agcgtttgcg gatgtgcttg aggcgtcggc ggaagtttcg cttcagaagg gcctcatcgc
     8581 cgaggtaatc gaggtattcc tggccggtga aataggcgcc ctggtagatc tgtcgggcat
     8641 cgatggggtt ttcgagacgg gcagtcacaa acccacagac cgggcacttg agcagaatgt
     8701 ccaggaaagg ctcgaatccc tgatgagagc agaccatgca ggccggcggc atgtgggtta
     8761 ccccatgtca gaagaccgtc agccgacctc tcgcttctgg aacagggcga cggcaatcag
     8821 aagccaggcc agaacgatca gcatcgcgta gccaaatgcc atgcccacgt atggcgcggt
     8881 gatacggttg ttctgggtca aggcatctgc cacccagaga aacgccaagt tgggcgagag
     8941 ccaataggca aatcgggcca ggaggctggt atcgcgaagt tgcccaaaca gatagtcgga
     9001 ggccaaaccg atcatcagga ccagtgtgca gatcgccagc gtggccactt ggccgaggcg
     9061 agtcgagact gccaccgcca cggccgtggc gatcatgacc atggcaaaga ccagggcaac
     9121 ggccccgatg atttgaccgt cgatgaagtc tttgccgaat tgctgtattt cccacgtagg
     9181 accgatcaga cacaccaaga ccagagccaa ggtcatcaac gggattgaga tagttaccga
     9241 tgtcgacgag aactgcatgt catacaagta gttacagaga gcggcgatca gcagtgtggt
     9301 aaagaaggca gcgcatccga acacgatcac gggcacatcg aagtggtcag agctgcggga
     9361 cattaccttg tgccgcatgg tcaacaggaa gacgatgccg ttgagatagt aggctatagc
     9421 cagggccccg gacaagccgg cgaacttgcc cagaatgaac acgggacggc cgaccggctt
     9481 actgacgacg gtcaggaccg tcttgttttc gatttcgcgc gacaaaaccc ctgcggcact
     9541 gaaggccgcc aaaaacaaac cgcacacaag catcgtagaa aggctaaggt ctttcaggag
     9601 cttgttgtcg tcatcgaagg tgtagccggc caaaccgagg ttgagcacca acacgaaggt
     9661 gctcgcaagg aggaggattc cgtagattgg ctgtcttacg gtttctgtga gactgttggt
     9721 agcaataccc aagaacttgg tgaacatggc gcccaactat cagcaagaac agaggttaca
     9781 ctttatcgat cgcaacggac gaaacgctcc aaggcggccg ggcccaaact tggcaggccg
     9841 acccgcatat tgtagagtac gcggctcggc cgtccactgc tcggcggatt tccgaattga
     9901 gctggcaaag atgtgcgtag agccaaccac atgaggcgcc attggagatg gcgactcacc
     9961 agagaaacag agcatcatga caacgcaaag gcctgaacgt agagcccgta ctgtagcact
    10021 cgccggtctg gcttttcaga tcctgctgtc ggtcttcttc gcggtcttgt ggtcgtgggg
    10081 gaagacggag ggactgcgag ccctcatgct gctgtcggcc atcgggaacg cgatatggct
    10141 gttccttgtc atggtctgcc agcagcgcgt cctggttcag gaagagaagt tcgagacgga
    10201 gcagcttcgg cgggaacgag cggcggcagg cggcaccgat gccatcttcg acgtcgcgga
    10261 cgaagagctt cttctggctc gccggcgact gcagtggatg tatcggtgga tgctgccgag
    10321 cttttcgatc ctggtgattg tgctgctggt tgtggccggt cttgtgggat ggagatggtc
    10381 gatcggtgac tcgttctcct ccgagaattg gacggccgtc ggcaaggaca agatcccgct
    10441 gttgttcttc atgacgcttg gggcggcttt cgtgagtttc ctgttttcgc gatacgcgac
    10501 cggcatggct cgctaccgcg agtggcagat cctgcacgcg ggcgccacct ggctgatggg
    10561 cgtcacgctg gcatcggtgc ttgtcagtgc cgccatgggt gcgctttaca tgggggcgac
    10621 gcccgttttc gagcgg
//
LOCUS       DAOTPW010000201        10584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5884_length_10584_cov_5.641941, whole genome shotgun sequence.
ACCESSION   DAOTPW010000201 DAOTPW010000000
VERSION     DAOTPW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5884_length_10584_cov_5.641941"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..716
                     /locus_tag="P5316_16220"
     CDS             54..716
                     /locus_tag="P5316_16220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR86557.1"
                     /translation="MHPEGIPWPGTVLYDALSRSRIFQEHYRLLAEDIAAHCRQGSIL
                     DIGTGPGRLLLSLHELRPDRRLIGVDVSTAMARQAQENVRRAGLSNVIQILAAPAQRL
                     PFADQSFDVVVSSGALHHWKNATAGLNEVYRVLRTGGWALMYDLVKDTPPKILQGLTK
                     AHGRFRVRLLQLHSLEEPFHTVGDLASLAEATPFRTEQVRFVGALCCLALTKPAPKSG
                     EL"
     gene            1128..2672
                     /locus_tag="P5316_16225"
     CDS             1128..2672
                     /locus_tag="P5316_16225"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRR86558.1"
                     /translation="MISPAGAASPKNVIFMIGDGMGFEHVKAGGMYLNGQPGTLCFES
                     LPYRAQVTTYSADNSVTDSAAAGTAMATGHKVNNGVVSMAYPGDGRELQTLLELYKAR
                     GTRTGLVTSDAMTGATPAAFGAHEYNRDNRAQIAGDYLNQTRPNVLLGGGGNGITTSA
                     ASAAGYTVVTDRAGMQAVDTNLISFLSGQFGTAEYMPYEYDGLGNLPHLSQMIVTALD
                     ILDNEPNGFLLMVEGGNIDHAAHANHIARVVYEVVEFHNTVQIALNWAAGRTDTLILV
                     TADHETGGLTVTQNNGQGNLPTVSWSSTGHTAAQVGAWAWGVNAQYVTGTLNNTAFFE
                     IIAGPEPVMLLSPKDISRSVAWAHNPPPQTDSFTLSNARLGTYNYTITSDASWVWADP
                     ASGDCSTETDTIWLVYDVGLLLPGDYVAHLEIRSDEAINAPQILTVTLTVRPAPGDFN
                     RDNDVDQEDFGYLQGCLTPVGASLPPECRNADLTSDGRVDASDIVVFRACLSGPDVQP
                     DPYCAD"
     gene            3104..4072
                     /locus_tag="P5316_16230"
     CDS             3104..4072
                     /locus_tag="P5316_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRR86559.1"
                     /translation="MPALDNTTAQREKQWAAGSSVLAAIALTLLKVVVGLMTGSLGIL
                     AEAAHSALDLVTALVTLFAVRASDKPADQEHLYGHGKIENVSALFETILLLLTCGWIA
                     YEAVHRLIAKHVHVDASIWAFGVMAVSILVDVSRSRVLHRTARKYGSQALEADALHFS
                     TDVWSSSVVILGLIGVRLGDVYPRHGAWLSRADSVAALGVSLIVIWVSLRLGKRTLQA
                     LLDTAPAGLSEQIRTAVDGVPGASGCHRVRVRPSGPGLFVDIHVYVDGSTTLASAHDL
                     TEAIEKRIGEVVPGADVTVHPEPRPTDENDEKEPLPGTIRPLQDGE"
     gene            4120..4554
                     /locus_tag="P5316_16235"
     CDS             4120..4554
                     /locus_tag="P5316_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86560.1"
                     /translation="MPEEKLGAPAPVALAMIVCDAIYQDPATKKCTLLGTFSTITARR
                     FPVVHPQLAVHVVLTDGRGNVRIKLALVGDNETHPPLFAGEGVIHFADPRVVAELNFQ
                     LSNVTFPQPGAYRFQLFANNELLMERRLLVLQGGPPPSEPPI"
     gene            5365..5868
                     /locus_tag="P5316_16240"
     CDS             5365..5868
                     /locus_tag="P5316_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86561.1"
                     /translation="MGDEYIAGFMIFTGTYELVVDPKNRLSIPASIRSQMDPEKDGTR
                     FYLVPGSRRGTLALYADRYFEQYAETYHASLTANDEKEDFEQVFYAMATLLDIDRQGR
                     VVLPQRALEYTGLGRKVTLAGQRDHLVIWNREQFGRFIEENWSRYPDLLRQARLKTEV
                     SKLNGAR"
     gene            6454..7395
                     /gene="rsmH"
                     /locus_tag="P5316_16245"
     CDS             6454..7395
                     /gene="rsmH"
                     /locus_tag="P5316_16245"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRR86562.1"
                     /translation="MNRDQIEHEPVLCGPLMAWTAPRPGEIVVDATVGHGGHACLLAR
                     AIGPAGRLIALDVDAYNLGRAKARLERAGLAGGGPHIDLIRDNFANLGDVLDRLGVDR
                     VDVIVVDLGVSTDQLLGGGRGLTFAESEPLDMRLDDRLTITAADLINSLSERELADLI
                     YSHSQERRSRRIARRICQARRDGPIRTTAELVRIVCSALGVSVHSRPGRLHPATRTFM
                     ALRMAVNRETENLTTLLKLAPTRLSPGGRIAVISFHSGEDRLVKRDFLARQRLGVYEV
                     RTKKPIRPSDEEVNFNPRSRSAKLRVAVRTADVQNVA"
     gene            7420..8895
                     /locus_tag="P5316_16250"
     CDS             7420..8895
                     /locus_tag="P5316_16250"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRR86563.1"
                     /translation="MRSAWGNDAWDIAVCIVAGLHALAVPQRRSVSGLRPLPAPRNPL
                     FKDGFFTFDTEVSMTRETKIGLLMGLGFIVVFAVLLLQTGNHPPSGDLQMMLSRHGNS
                     AASRPETLARLPEPIIPRSTATSPAVSTGEHVAESLTRATEPWNRGLPNPAVFNAKPD
                     YRELGPGSSELTEALMPQPRVGPDAPTVAPVRVAPTNDVTPLSRPDGPKPSPEPDPPK
                     TPPEVQRSQTSPSLPVDRLPVTSELTVPATYVVQKGDNLMRIARKHYNSQSTEVVDFL
                     LKSNRDRIRDRHVVQTGQTLLIPVLPVNLRNGSMDSDVTVGRSPVRPEDPGSSSPDSE
                     GSTGLRLLHAERVVPPPEKLLDAYQRKGNAEVMAKARTVPDAVDKPKDARNTGDHDSL
                     ASATGNKAKSEQGNEPKASSGEKRSISSEKSDDRSYRWYTIRPRDTLALIASKELGGS
                     ANWEEIVKLNKNINPAKLKVGDRIRLPERKPTSDSSKRSST"
     gene            8892..9179
                     /locus_tag="P5316_16255"
     CDS             8892..9179
                     /locus_tag="P5316_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86564.1"
                     /translation="MTIPRMIGLLIGLTMIGLAVVSARVERARHDRRLQELQIEQARV
                     NERISQQEMDLARLKSPEMIRQRAAELGLLEEAQKSGQKNSGMMPGTPGRR"
     gene            9201..>10584
                     /locus_tag="P5316_16260"
     CDS             9201..>10584
                     /locus_tag="P5316_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRR86565.1"
                     /translation="MGSAAAQRMRVAQWIIYLICGALALLVGRLVHLRAKEAPFWQRY
                     AQERQISTIPMPARRGFVFDRRFRVLAGSQELPTVFADPRPMEDRGEAAQALSGILNM
                     PAREIRALLDEPTAPGYVVLKRSVDARVVEEIKKAKIVGIGVHNEPFRTYPMGSLASH
                     VVGFVGKDGNGLEGVEKYFEAYLHATPGKRDVYRDVRRRAVLPVPDSSVAPRDGDNVV
                     LSIDVAIQEMVEKAVAEQVARFQAECGLGVVMDPKTGEVLAMTNQPTYAPSEGSEVPP
                     ERRRNRVLTDPVEPGSIFKPFVMVTALAEGVTTPTESIFCENGLYAVGARRLHDHHPY
                     GFLTTAEVLAKSSNIGMAKLGERLGNQRMYEALYALGFGHPTGIDLPGEDPGLLMPLR
                     KWDKFTTTSVPMGQEIAVTPMQILTAFCALVNGGRLIQPRVVLAVIDRDGKLVRDNSR
                     VIDKGQRINPT"
BASE COUNT         2094 a         3125 c         3322 g         2043 t
ORIGIN      
        1 gcaagcgagt ccgcaaaagg atgtgcgtaa tgtggagcga ccttctcaag cgaatgcatc
       61 cggaaggcat tccctggccc gggacagtgt tatacgacgc attgagtcgc agccgcatct
      121 tccaggagca ctatcgtctg ttggccgagg atatcgccgc ccattgccgg caaggaagca
      181 tactggacat cggtaccggc ccaggccggt tgctgttgag tcttcacgaa ctccgtccgg
      241 accgccgctt aataggagtc gacgtgagca cggcaatggc gcgccaggcg caggagaacg
      301 tgcgccgtgc aggactgtcc aatgtgattc agattcttgc cgcgccggcg cagcgtttgc
      361 cgttcgccga tcaatcattt gatgtcgtcg tcagcagcgg cgcgcttcat cactggaaga
      421 atgcgacggc cggactcaac gaagtgtacc gggtcttacg aacaggcggt tgggcgctca
      481 tgtatgatct cgtgaaagac acgccgccaa aaatcctcca aggactgaca aaagcccatg
      541 gtcggttccg cgtgcgcctt cttcaacttc actcccttga agagccgttt cacactgtcg
      601 gtgatctggc gtcactggcc gaggccacgc cgttcaggac cgaacaggtg cggtttgtcg
      661 gagccttgtg ctgcctggcg ctcaccaagc cggcgcctaa gtcaggggaa ctgtaagacc
      721 ggcaatgacg acgttgcctg tccggctggg cggatgccag acccgctcag gcgacagatt
      781 ctttgagatt caggcttttt ctgtggcggc tgtctgacta aaatgagcct cgtcgtccta
      841 tccgtcgtcg gcgtggtctc tggggaggaa ccgcgacgag gcagcgactc caaaaagatt
      901 ccgggcggtg acgcatgtcc agacatagcc tgagagcgcg tgtcagaagc agcggggccg
      961 gccgggcggt agggcagcgt cgagatcgcc acggcgaccg agacctgccg tatctgccgg
     1021 ccccaggccg agcggaaggt caccgccgcc gtcggcggct ccggccatgc cctactctcc
     1081 tcgcacacgc tctgagtcga atcctgaccg gtcttgtgct tgtttgcatg atcagccctg
     1141 ccggggcggc ttccccgaag aacgtcattt tcatgatcgg tgacgggatg ggtttcgagc
     1201 atgtgaaggc cggcggcatg tatctcaatg gccagcccgg aactctttgc ttcgagtcgc
     1261 tgccttatcg ggcccaggtt acgacctact cggccgacaa ctcagttacg gactccgccg
     1321 cagccggcac cgccatggcc accggccaca aggtcaacaa tggcgtggtt tccatggcct
     1381 acccgggtga tggaagagag ctgcagacgc tcctcgaact ctacaaggcc cgtggaacac
     1441 gcaccggttt ggttacctcg gatgcgatga ccggtgcaac acccgccgct ttcggggccc
     1501 acgagtacaa ccgggacaac agagcacaga ttgccggtga ttaccttaac cagacccggc
     1561 ccaacgtctt gctcggcgga ggtggcaacg gcatcacgac ctcggcggcc tccgcggccg
     1621 gttacaccgt cgtaacggat cgcgccggca tgcaggctgt tgacaccaat ctcatcagct
     1681 tcctctcggg ccagtttggc accgctgagt acatgccgta cgagtacgac ggtctcggca
     1741 acctgccgca cctttcccag atgatcgtca cggccttgga tatcctcgac aatgagccga
     1801 atggcttctt gctcatggtc gaagggggaa acatagacca tgctgcgcac gcgaaccata
     1861 tcgcacgcgt cgtatacgag gtcgttgagt tccacaacac cgtccagatt gctctcaact
     1921 gggccgcggg ccggactgat acgctgattc tcgtcaccgc cgaccacgaa accggcgggc
     1981 tgaccgtcac gcagaacaat ggtcagggca acctccccac cgtttcctgg tcctcgacag
     2041 gtcataccgc cgctcaggtt ggggcgtggg cctggggcgt gaatgcccag tacgttaccg
     2101 gcacgctgaa caacaccgcg ttctttgaga tcatcgcggg tcccgaaccg gttatgttgc
     2161 tctcgccgaa ggatatttcg agaagcgtcg cctgggctca caacccgccg ccccagaccg
     2221 attcgtttac gctgagcaac gcccgcctgg gaacctacaa ctacacgata accagcgacg
     2281 cgagctgggt ctgggccgat cctgcatccg gagactgctc gaccgagacg gacaccatct
     2341 ggctggtcta cgatgtcggt ttgctgctgc ccggtgacta tgtcgcccac ctggagatcc
     2401 gaagtgatga ggcaatcaac gcaccccaga tactcacggt caccctgacc gtccggccgg
     2461 caccaggcga tttcaacagg gacaacgatg ttgatcagga ggacttcggc tatcttcagg
     2521 gatgtttgac gcccgtcggc gcttccctcc cgcccgagtg ccggaacgcg gatctcacct
     2581 cagacggtcg tgtcgacgca tcggatattg tcgtcttccg agcctgcctg agcggacccg
     2641 acgtccagcc ggacccctat tgcgcagact gaatgcgccc gcttaccgtt ctgtcacccg
     2701 accggctccg aatcctttgt cggccggact gccggtttgg catcttaaga aactcgccgg
     2761 acaaacgctg cgatggtgtt gagaggttct tttacaaccg cgaccgcagg tgagcacccc
     2821 gattgtcgtg tgcccgcgtt gcggtccggc ccattgtgcg ctcggaggtt ctgaaagagc
     2881 cgtccagaga gccacaagcc ctcgacaagg ccggcccgga aagcgtgctt gaccgaacca
     2941 tcggacgctg agatagtaga ctgatcggtg agcgggccgc tcgataagag cggccccaat
     3001 cgttgcgtcc ggtccgacga ggaagccgaa gccgcgttga cttttgaagg cctgatgatc
     3061 ggtctggcga aatgagtatc gcgaggctga ggagagctaa ggcgtgcctg ctctggacaa
     3121 cacgaccgcg cagcgagaga agcaatgggc ggccggcagt tctgtcctgg ctgcgattgc
     3181 gcttacccta cttaaagttg tcgtaggcct gatgaccggc agtctgggca ttctggccga
     3241 ggcggctcac tcggcgctcg atctggtcac cgccctggtg acgctgttcg ccgttcgggc
     3301 ttccgacaag ccggccgatc aagagcatct ctacggccac gggaagatcg agaacgtatc
     3361 tgctctcttc gagaccattc tgttgctgct gacctgcgga tggattgcct acgaagccgt
     3421 gcatcgactg atcgccaagc acgtgcacgt ggatgcctcg atctgggcat tcggcgtcat
     3481 ggccgtttcc atcttggtgg acgtgtcgcg gagcagggtc ctgcacagga ccgctcgcaa
     3541 atacggaagt caggctcttg aagccgacgc cctgcatttc agcacggatg tctggagctc
     3601 ttcggtggtg atcctcggcc tgattggagt tcgacttgga gacgtttatc cccggcacgg
     3661 cgcctggctt tcaagggcgg actcggttgc ggcccttggg gtgtcgctga ttgtcatttg
     3721 ggtcagcctt cgactcggca aacgtaccct gcaggccctg ctcgacacgg ccccggccgg
     3781 tctgtcggag cagatacgca cggcggtgga cggcgttccc ggagccagcg gctgtcaccg
     3841 cgttcgcgtg cgaccatccg gtcccggtct ctttgtcgac atccacgtct atgtggacgg
     3901 cagcacgacg ttggccagtg ctcatgacct caccgaggca atcgagaaac gaattggtga
     3961 agtcgttccc ggtgccgatg tgaccgtaca ccccgagccc aggccgaccg acgaaaacga
     4021 cgaaaaagaa cccttgcccg ggacgattcg tccgttgcag gatggggagt gacagactcc
     4081 ccgccggacc gtagcatgtc ggctgtggag gacgcggcaa tgcctgagga aaagcttggc
     4141 gcgccggcgc cggtagcgct ggccatgatt gtctgtgatg ctatttatca ggatcctgcg
     4201 accaagaagt gcacgttgct cggcactttc tcgaccatta cggctcgccg ctttccggtg
     4261 gtacatcccc agttggccgt gcatgtcgtc ctgaccgacg gtcgaggcaa tgtgcggatc
     4321 aagctcgccc tggtggggga caacgagacc catccgccgt tgttcgccgg cgaaggcgtg
     4381 attcactttg ccgacccgcg ggtggtggcc gagctgaatt tccagttgag caacgtgacg
     4441 tttccccagc ccggcgccta tcgctttcag ctctttgcca acaacgaact gctgatggaa
     4501 cggcggctgt tggtgttgca gggaggaccg cccccttccg agccgccaat ctgaccatgc
     4561 gtgcctcatt gcgccgagcg aaatcaggtc aacttgcgga gtccgccatc cgtttcggcg
     4621 atcgattcgt aggcggatcc gtggattaaa ccgtcgacgg tggtaggcgc atcatccgtt
     4681 ttgaacaaaa cttagaggca ttcgatgctg caacgggctg atgcaaatgc cagggcgcaa
     4741 agaactcccc aagacggtaa tgcgtcgcct tggcactcaa gcaagttcgg tttttgtaca
     4801 gacgctggga acctgccttt cggtttgatg gctcaatagt ctccgcaggc cagacttgga
     4861 cagctcaagc ccgagtccaa tgcgaccgac tccgcgacgt gttgggcgcg gaagaagtcg
     4921 cgatctcggc cgatccagcc gaagatcaaa tcgctccgtg agacgacatc cgggtttcca
     4981 cccattcgcg ggcgtggcag attgttcccg cgcgcgcccc cactttctac cacgttcagg
     5041 ggtttactcc cgacgtctcg atcaccccag gccgcgcaca accgctctct gatcgccatc
     5101 tccggtcgcg ggcgcgttca tggcattgtg ttcatggcat ttgaaccaat gcggctgagc
     5161 ctgaacacgg cgcacttttt gcgcatgggg tttctgcagg cacgattcat ttgctgacca
     5221 agatcgccga gccccatctg tggggcgaac aaatcccgaa agaaaaaaag aaaaaaaacg
     5281 cgcgattatt ctccaaaaag gacttgcaat cgagtgggtc catgtgttat gtattcccat
     5341 cattgagcat cgaaatggat tgatgtgggt gacgagtaca tcgcgggttt catgattttc
     5401 accgggacct acgagcttgt ggttgatccg aaaaaccgtc tgtcgatccc tgccagtatt
     5461 cgatcccaaa tggaccccga gaaggacgga acgcgtttct atctggtgcc tggcagtcgc
     5521 cgaggaacgt tggccttata cgctgatcgc tactttgagc agtatgccga gacctaccac
     5581 gcttcattga cggccaacga tgagaaggag gatttcgagc aggtcttcta cgcaatggcc
     5641 accctgctag acatcgacag gcaaggtcgg gtggtcttgc cccaaagggc tctggaatac
     5701 acgggccttg ggaggaaagt cacgctggcc ggtcagcggg atcacttggt gatctggaat
     5761 cgggagcagt ttggacgctt tattgaagag aactggagtc gctaccccga tctgctgcga
     5821 caggcaagac tgaaaacaga ggtaagcaag ctcaacggtg cgcgttgagg attgcgaacc
     5881 ttgaaagtag gccggtcgaa tgaaggccgg gtcattggcg agagcgaggg aacggtcagg
     5941 gaagggggtt cgacaggcgc ggatcggatc ctcttcccga ccagcccata atcattaaag
     6001 acggggacgt cgggatttcc tgaacgaggt ctctgtggga gtcgcagcga gtggaacaag
     6061 cggctggatg gttagtgggg gcgcgatcag tcgtcgtgtg agatgtgggt cgcgcttgag
     6121 cctgagggat tcagcagcgg aagcgaacgg agtcgccggg ctgagcacac tccctgattg
     6181 tcggatcggt ttccactggc agttgcggct agcgtcaggg ggcgggcatt gtttttcgcg
     6241 gggccatcca agaccggacc cgaggacgcg gacgggtcgg cggtggccca gagatgcgag
     6301 aaacctgtgc tcgcgcaacc ctcggaagac gcggccaata ccgtcagcga tcagggagta
     6361 cgggtacggg tcgccaggga ggtgtgaccg tgccctcttg atgcaagatt gggaggaatc
     6421 aacccatatc cggcgtcggg gtgcgggctg gtcgtgaatc gcgatcagat cgagcatgaa
     6481 cccgtgttat gtgggcccct gatggcatgg acggccccca gacccggcga gatcgttgtg
     6541 gacgccaccg tgggccatgg cggccatgct tgcctgctgg cgcgggcgat cggtccggcc
     6601 ggccggctga tcgcactgga cgttgacgcc tacaacttgg gcagggccaa ggccaggctc
     6661 gaacgcgcgg gtttggccgg gggcggcccc catatcgatc tgatccgcga caacttcgca
     6721 aacctgggtg atgtgctgga tcggcttggc gtcgatagag tggatgtcat cgttgtcgac
     6781 ctcggtgtca gtactgatca actgctcggc ggggggcgcg gcctgacctt cgcggagtcg
     6841 gaaccgttgg acatgcggct ggatgatcgg ctgaccatca ccgcagccga cctgattaac
     6901 tcgttgagcg agcgcgaatt ggccgatcta atctatagtc acagccagga acgtcgtagc
     6961 cgacgaatag caaggcgaat ctgtcaggcg cgtcgcgacg gaccgattcg gaccaccgcc
     7021 gagcttgtga gaatcgtctg ctcggcgctg ggcgtgtcgg tgcactcgcg ccccggaagg
     7081 ctgcacccgg caacgcgaac cttcatggcg ttacggatgg cggtgaatcg ggagaccgag
     7141 aatctcacaa ccttgttgaa gctggcgccg acgcggctgt cgcccggcgg aagaatcgcc
     7201 gtgatcagtt ttcacagcgg cgaagaccgg ctggtcaaga gagactttct tgcacggcag
     7261 cgacttggcg tgtatgaagt aagaacgaag aagccgatcc gtccttcgga cgaagaggta
     7321 aacttcaacc cccggtcgcg cagcgcgaaa cttcgcgtcg ccgtgcgtac cgcagatgtg
     7381 cagaacgtgg cctgactgaa cgtcgccggc gcgggcggct tgaggtcggc gtgggggaat
     7441 gacgcctggg atatcgctgt ctgtatcgtg gccggcctgc atgcgttggc cgtgccgcaa
     7501 cgacgaagcg tgagcggttt gcgaccgttg ccggcgcctc gcaatccgtt gttcaaggac
     7561 gggttcttca cgtttgacac ggaggtttcg atgactcgcg agacgaaaat cgggctgctg
     7621 atgggtctgg gcttcatcgt ggtctttgcg gtgttgctgt tgcagaccgg caatcatcca
     7681 ccgagcggcg atctccagat gatgttgtcg cggcacggca actcggccgc cagtcggcct
     7741 gagacgcttg ctcggctgcc cgaaccgatc attccgcgaa gcacggccac gagcccggct
     7801 gtgtctacgg gggagcatgt tgcggagtca ctgactcggg caacggaacc gtggaatagg
     7861 gggctgccga accccgcggt ctttaacgcc aagccggact atcgtgagct ggggcccggc
     7921 agctctgagc tgaccgaggc tctaatgccc caaccgcggg tcgggccgga tgccccgacg
     7981 gtcgcgccgg ttcgcgtcgc tccgacgaat gacgtgacac ctttgtcccg accggacggg
     8041 ccgaaaccat cgcccgagcc ggacccgccc aaaacgcctc ccgaggtgca acgatctcaa
     8101 acatcgcctt cgcttccggt tgaccgtttg cctgtcacga gtgagttgac cgtgcctgcc
     8161 acctacgtgg tacaaaaggg cgacaacctc atgaggattg ccaggaagca ctacaacagc
     8221 cagtccaccg aggttgtgga ctttcttttg aaatcgaacc gcgacaggat ccgcgaccgg
     8281 cacgtcgtgc agacaggcca aaccctcctg attcccgtgc tacccgtgaa cctcaggaac
     8341 ggctccatgg acagcgatgt aacggtcggt cgcagtccgg ttcgccctga ggacccgggc
     8401 tcatcatcac ccgattcgga gggcagcacg gggcttcggc tgcttcacgc cgaacgggtt
     8461 gttcccccgc cggagaagct gcttgacgcc taccagagga agggcaacgc ggaggtgatg
     8521 gcgaaggcac gcacggtgcc ggatgccgtg gacaagccga aggacgctcg caacaccggt
     8581 gaccacgatt ctctggcatc tgcaacgggc aacaaggcca aatccgagca gggcaacgag
     8641 cccaaagcct cctcggggga gaaacggtcg atttcctcgg agaagtcgga tgatcgcagt
     8701 tatcgctggt acaccattcg gccgagagat accctggccc tgattgccag caaggaactg
     8761 ggcggatcgg caaactggga agaaatcgtc aaactcaaca agaacatcaa tccggcgaag
     8821 ctgaaagtcg gcgacaggat tcgcctgcct gagcgcaagc cgacctcgga ttcgtcgaaa
     8881 cggtcgtcga catgaccatt ccccggatga tcggtcttct gatcggcctg accatgatcg
     8941 gtctggcggt ggtgagtgca cgggtcgagc gagcccggca cgatcgacgt cttcaggagc
     9001 tgcaaatcga gcaggcccgg gtcaacgaac gtatttctca gcaggaaatg gatctggcca
     9061 ggctgaaatc gcccgagatg atccgccagc gggcggcaga acttggtttg ctcgaagaag
     9121 ctcagaagag cggacagaaa aactccggca tgatgccggg aacaccgggc cgacgctgac
     9181 ggaacgtagg aggttgctcc gtgggatcgg cagccgcaca acgaatgcgg gtcgcacagt
     9241 ggatcatcta tctgatctgc ggggcattgg cgctgctggt cgggcgtctg gttcatctgc
     9301 gggccaagga agcgcctttc tggcagcgat atgctcagga acggcagatc agcaccattc
     9361 ccatgcctgc ccgacggggt ttcgttttcg accggcgatt tcgcgtgctg gccggaagcc
     9421 aggagcttcc aacggtcttt gccgatccac gacccatgga ggacagaggg gaggcggctc
     9481 aagccctcag cggtatcctg aacatgccgg cccgtgagat tcgggcactt ctggacgagc
     9541 ccactgcacc cggctacgtc gttctcaagc ggtcggtgga tgccagggtg gtggaggaga
     9601 tcaaaaaagc caagatcgtc ggcatcggcg tccacaatga gccttttcgc acctacccca
     9661 tgggttcgct ggcttctcac gtcgtcggct ttgtcggaaa ggacggcaat gggctggagg
     9721 gcgtcgagaa gtattttgag gcctatctcc acgcgacgcc cggcaagcgg gacgtctacc
     9781 gcgacgtgcg acggcgggcg gtgcttcccg tgcctgattc ttccgttgcc ccgcgggacg
     9841 gtgacaacgt cgtgctctcc atcgacgtcg ctattcagga aatggttgag aaggcggttg
     9901 cggagcaggt cgcacgtttt caggccgagt gcggcctggg cgtggtcatg gatccaaaga
     9961 ccggcgaggt gctcgctatg accaaccagc cgacctatgc gccttccgaa ggttcggagg
    10021 tcccgccgga acggcgaaga aaccgcgtac tcaccgatcc ggtggagccg ggtagtatct
    10081 tcaagccctt cgtgatggtg accgctctgg ccgaaggcgt gacgacgcca accgagtcca
    10141 tcttctgtga aaacggcttg tatgccgtcg gggctcgccg cttgcacgac catcatcctt
    10201 acggctttct gaccacggca gaggttctgg ccaagtccag caacatcggc atggcgaaac
    10261 tgggggaacg tctgggcaac caaaggatgt acgaggcgct ctatgcgctg ggcttcggcc
    10321 acccgacggg tatcgacctg ccgggtgagg accccggcct gctcatgccc ttgcggaaat
    10381 gggacaagtt cacgacgacc tcggttccca tggggcagga aatcgcggtg acacccatgc
    10441 agatcttgac ggcattctgc gccttggtca acggaggccg tctgattcag ccgcgcgtgg
    10501 ttctggctgt gatcgaccgc gacggaaagc tggtgcggga caacagccgt gtaatcgaca
    10561 agggtcagag aatcaacccg acgg
//
LOCUS       DAOTPW010000202        10500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5933_length_10500_cov_6.002202, whole genome shotgun sequence.
ACCESSION   DAOTPW010000202 DAOTPW010000000
VERSION     DAOTPW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5933_length_10500_cov_6.002202"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="P5316_16265"
     CDS             <1..250
                     /locus_tag="P5316_16265"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR86566.1"
                     /translation="DLGALKKKYGRKMVFNGAIDSHHILINGTPETVRHKTREVLEIM
                     KPGGGYIAGASHDSILEETPVENVVAMFDAIREFGAYR"
     gene            complement(554..1894)
                     /gene="glmM"
                     /locus_tag="P5316_16270"
     CDS             complement(554..1894)
                     /gene="glmM"
                     /locus_tag="P5316_16270"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRR86567.1"
                     /translation="MMTVSGVRGVIGEMLTPTLAAELGCAFGTYLRGGKVVVGRDSRP
                     SGAMVQQGVVSGLLAAGCDVILLGIASTPATAMMVRRHSANGGVVITASHNPVMWNGI
                     KFLTSEGLAPPPEQAAKIFGIYDLKAFTLAGVEHLSTPQSDPSAANAHVEAVLSLVDR
                     KLISAKEYRVVLDSINGAGGVEGKMLLDNLGCEVVHINAEANGRFAHAPEPLAENLAQ
                     LCDAVREKQAIAGFAQDPDADRLAIVDENGIYIGEEYTLALAARYMFAAHPGPAAANL
                     STSRMIDDVAAQAGGACRVYRSPVGEANVVAVMKDHGCQFGGEGNGGIIDLRVGPVRD
                     SLVAMALTLQLMTLTGKTISQLVADIPRYVMIKQKFDCPKERVERILDAVRTQCCDGQ
                     INDIDGVRVDYPDGWVHIRGSNTEPIVRIIAEAPTQDRAHDLINRMRQIMDATP"
     gene            complement(1903..2955)
                     /locus_tag="P5316_16275"
     CDS             complement(1903..2955)
                     /locus_tag="P5316_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86568.1"
                     /translation="MVCRGTHGHGLAHRMGWVAATCSVFGSFAAGQTTRKADAPVPVS
                     QPTYSRVEKIPEELFDDSRRSVILLPSTPGEVGRPAEDRSQGARKQIVPETPLLPEGY
                     VVASREARIETEGDWLIAHLRKSTDLPDGPPLRILPNQQLSMLRAVLKQSPPPTSFLM
                     TGRITEFMGANYILLENIEEVCADGAEAGAPAEAAPQSTPADATRDREPTAEEILRQL
                     LSRPTRRSVALPQLTQIIQGDDGRAVTTESRPEDPGAAAGLLPERAMLVDRVGRVVPG
                     DKWWTLVFENRGRQAADQPIRLLPSRLLESAISLSRGGTQAPVFIVSGEVTTYESRNY
                     LLLRKVLVRRQVGNIR"
     gene            complement(3081..5210)
                     /locus_tag="P5316_16280"
     CDS             complement(3081..5210)
                     /locus_tag="P5316_16280"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRR86569.1"
                     /translation="MTDRTASQAAARANRLADSTSPYLLQHARNPVDWFPWGTEALEK
                     ARREDKPIFLSSGYAACHWCHVMEQESFENPAIAAILNEHFVSIKVDREQRPDLDDLY
                     MKATLLCNQGNGGWPMSVFLLPDDQTPFFAGTYYPPTSRYGRIGFADLLRRIAGLWHD
                     DRNEIRRAAASLVEGVRQLSRPAPLDTAITADMVSSAAAALASAFDPVQGGISGGGTN
                     KFPPSIAMSLMLREYVHSCRRGRPDPILLERVTLTLDKMANGGIYDHLAGGIARYSTD
                     PSWLVPHFEKMLYDQALVSGVYLEAFQVTGDRRYADVASRILDYVLADMRSPEGAFYS
                     AWDADSEGIEGKYYVWTKSEIMAVLGEHEGEVFCTYYNVTEQGNWEGANILNVQRAPD
                     VVARLYGISETQLSRMLRIAREKLLLARRQRVPPALDDKTLTAWNALVITSLARAAEV
                     LSEPRYADAAATAAEFILTNLIADGRLLRTWRNGKAHTPAYLDDYAFLVEALLELHQA
                     TLDWRWLERAAQINDEMLRLFWDDREGACYFTPSDAERLFVRTKDFRDGAIPSGNSVA
                     IMNLLRLAAILDEDDLRVKAEQSVRAVAGDLHHGPFGHERLLAAVDLLCSPSTEIVIV
                     GDRRDAATKELIAVTREGYDPARIVLVKDPSEHPAVPRQQVPALEGKTLLDGKASAHV
                     CHGRTCHVPVSSPAELRCLLNSLGRGA"
     gene            complement(5373..6164)
                     /locus_tag="P5316_16285"
     CDS             complement(5373..6164)
                     /locus_tag="P5316_16285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR86570.1"
                     /translation="MQATADTVCPQELEATPLSVASSAADIAGAPAEVHTSLEVLSEL
                     YNYNHWLFNKVRPFVGETVCEVGCGIGNITQFLLNRRQVVGIEPLAASLRQSTERFRD
                     HLNTRFFNGVLQDCPNETVPAGAFDTVLCMNVLEHIEDDVGALNTMRLLCGPHGRVVI
                     LVPAHMSIYGELDRAFGHCRRYNHKSLAAVFSKAGLLPTYGRYMNAIGYFGWFWEGRC
                     LRRQQINLQSARLFNRLVPFIDAFERLLPLPFGQSLLMVGRPESR"
     gene            6353..8116
                     /locus_tag="P5316_16290"
     CDS             6353..8116
                     /locus_tag="P5316_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86571.1"
                     /translation="MATLPDRDLPLLHAEPAMRHPVSKLAATALKAALPLTLCLMIAG
                     LLRGWGSGASFESSDQAMMPYLVRHSFGATWVFAHSYGPVPVIVHASVARILSHLGIP
                     MDEAVSRLPVAVLSITQVFLAYLLMRRLHCTRNLATAASLVCALLPPLVTDGHYAWGY
                     LTIWLLFGTTALWAALAYMDDRQTWHLSVASFSLFAHCLSNCFVFGVPLTLLIIWVTV
                     IRRAKQARRRVINDALFGFALPCLAAFAVAVMCRLGTGGGQIGRLLAKQHAGVMGWSP
                     TGMLKWLAIWSSQYGYLFVVPATAGLFHGVVLAKRGDRRGLLAIWAYTAFLPLLLLSN
                     PSRIGYPGAYLMEPVFAGGMLAVVLFDHLWNTLSRRTLILKTAFVAACLLSIAHLGFG
                     SIDSCLAGSRFRHWTGVTTGWGSLTPDSGIKAAGWYVRQYVPPDAVIMPLHTNRGMEA
                     PVAEYYLGRRVLAAMDVNADHLKPLLAAVVDHVDLVIAEPEYREMIEKSGDFVAVCTF
                     TRNGQAVRMIYARPAMQLPEMVQDVAVVNPQYDRLFAPSRVPLPLPAAPAYKAKMDIY
                     LRALRHLKSGRAPGPESGPAQ"
     gene            8238..9494
                     /locus_tag="P5316_16295"
     CDS             8238..9494
                     /locus_tag="P5316_16295"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRR86572.1"
                     /translation="MASRIDRRGKVKARQQADAGAAQVVVRDHLEELARDGARRMLTE
                     ALNEEVDAYLGRGRYERTKEYRGYRNGRTSRRLTLGAGTIELAAPRVRDIPEDQEPFE
                     SKILRKHQRRSDTIDATFLNLFVEGLATRDFEPALRLLVGADAPLSPSTISRLSRQFK
                     EQYEAFEVQDLSKRRFVYIWADGIYLKAGLGTEKACLMVLIGADTEGVKHLIALREGY
                     RESAESWGDLIRDCRKRGLNEPACWIADGALGLWAAVNAQSPRSAQQRCTNHKTMNVL
                     DKLPLKERPTWVPRIRAIWQADSEKAARKLAAAVIHDLREAGYDRAADCLGDDLDRCL
                     TFYCFPEAHWSHLRTTNPIESVFATVRLRTNAAKRFKKTRSGVWLMHQVLERLSKRWR
                     RLKSAHLCPTVPLPADTRKKTRTHAA"
     gene            9586..9888
                     /locus_tag="P5316_16300"
     CDS             9586..9888
                     /locus_tag="P5316_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86573.1"
                     /translation="MYGDPGDVLFQINELPRWQAAVYAIRYRICTGWHNFELGDEKTS
                     RKEAERLRDILAADWFLDRQKRREYRQRHPQYAGYTWRRLREEGPPGGAKARFGVR"
BASE COUNT         2013 a         3158 c         3359 g         1970 t
ORIGIN      
        1 ggacctgggg gcactcaaga agaaatacgg ccgcaagatg gtcttcaacg gggcgatcga
       61 ctcgcaccac atcctcatca acggcacacc cgagaccgtt cgccacaaga cgcgcgaggt
      121 gctggagatc atgaagcccg gcggcggcta catcgccgga gcgagccatg attcgattct
      181 cgaagaaaca ccggtcgaaa acgtagtggc aatgttcgac gcgataaggg aattcggcgc
      241 gtaccggtga ggcgtcgcgg cccgcagaca acctggtccg cgagcgcgga ccgtggggaa
      301 cgaggaatga tgaatgcaga attgagaacg ggactcgttt tgtcgcgaga gtcgcagcac
      361 cttgttgcga gccgcaacga aagcatgcgt tgacacagaa acatgcacgc aggatgcaca
      421 ccagtttcga ggaaagagac tatttggcga atacgagtcg cgggtccgca gccaactatg
      481 gagcgagacc tgccttgcct gctgcgtcca gacgttggag ggcgaatgcg caaccggcca
      541 gagagcagga cgtctagggt gttgcgtcca tgatttggcg catccggttg atgaggtcgt
      601 gggcgcgatc ctgggtggga gcctcagcga tgattcggac gatgggctcg gtgtttgaac
      661 cgcggatgtg aacccagcca tcgggataat cgacgcgaac accgtcgatg tcgttgatct
      721 gcccatcgca acattgcgtc cgcacggcgt cgaggatgcg ctcgacccgc tccttggggc
      781 agtcgaattt ctgcttgatc atgacgtagc gcggaatatc ggccacaagt tggctgatgg
      841 tcttgccggt gagggtcatg agctgcagcg tgagggccat agccaccagg ctgtcgcgaa
      901 cgggaccgac gcgaagatcg atgatgccgc cattaccctc gccgccaaac tggcagccgt
      961 gatccttcat gacggcaacg acattggcct cgccgaccgg cgagcgatag acacgacatg
     1021 ctccccctgc ctgggcggcc acgtcgtcga tcatgcggga ggtcgaaagg ttggccgcgg
     1081 ccggtccggg atgggcggcg aacatgtacc ttgccgccaa agccagcgtg tactcttccc
     1141 cgatatagat gccgttctcg tcgacgatcg ccaggcgatc ggcatcggga tcctgggcga
     1201 aacctgcgat ggcttgcttc tctcggacgg catcgcacag ttgggcaagg ttctcagcga
     1261 ggggctcggg cgcatgggca aagcggccgt tggcttcggc gttgatgtga accacttcgc
     1321 agccaaggtt gtccaacaac atcttgccct cgacgccgcc ggccccgttg atgctgtcca
     1381 ggacgacacg gtactccttt gccgagatca acttgcgatc aaccagcgac aggacggcct
     1441 cgacgtgagc attggccgcc gacggatcgc tctggggtgt gctcaggtgc tcgacgccgg
     1501 caagtgtaaa agccttaagg tcgtatatgc cgaagatctt cgcggcttgc tccggcggcg
     1561 gggccaggcc ctcgctggtg aggaacttga tgccgttcca catgaccgga ttgtggctgg
     1621 cagtaatcac gacgccgccg ttggccgaat ggcgccgtac catcatggcg gtcgcggggg
     1681 tgctggctat tcccaggaga atgacatcgc aaccggcagc cagcaggccg gagacaacgc
     1741 cttgctgcac catcgccccg ctcggacggc tgtctcgtcc gacaaccacc ttgccaccgc
     1801 ggaggtacgt cccgaatgcg cagcctagct cggcggccag cgtgggcgtc agcatttcgc
     1861 caatgacgcc gcgcactccc gaaacagtca tcatcagccg catcagcgaa tgttccctac
     1921 ttggcgtcgt accagaacct tgcgaagcag aaggtagttc cggctttcgt aggtggtcac
     1981 ttctccgctt acgatgaaaa ccggcgcctg cgttccgcct ctcgacagag agattgcgga
     2041 ttcaagcagt cgactgggca gaagcctgat cggttgatcc gcggcttgcc ggccgcgatt
     2101 ctcaaagacc agcgtccacc acttgtcgcc ggggaccacg cgtccgacgc gatccacgag
     2161 catggcccgc tcgggcaaca ggccggccgc ggcaccgggg tcttcggggc gagactccgt
     2221 cgtcaccgcc cgtccgtcgt cgccctgaat gatctgggtc agttgcggca aggctaccga
     2281 gcggcgcgta ggccgtgaga gcagttgcct aagaatctcc tcggcggtcg gttctcggtc
     2341 tcgggtcgcg tctgccggcg tcgattgtgg ggcggcttcc gcgggggcgc cggcctcggc
     2401 gccgtcggca caaacctcct caatattctc aagcaggata taattggcgc ccatgaactc
     2461 ggtgattcgc ccggtcatga ggaagctcgt cggcggcggc gactgcttca gcaccgcccg
     2521 gagcatggaa agctgctggt tgggcagtat gcgcagaggc gggccgtcgg gcagatcggt
     2581 cgactttcgc aggtgggcaa tgagccagtc accctcagtc tcgatccggg cctcgcgtga
     2641 ggcgacgaca taaccttcgg gaagaagagg cgtttcgggg acgatctgct tgcgggcgcc
     2701 ctgtgagcgg tcctccgcgg ggcggccgac ctcacccggg gtgctgggca gcagaatcac
     2761 acttcgccgc gagtcgtcga agagctcctc gggtatcttc tccacgcgtg agtaggtcgg
     2821 ctgggaaacc gggaccgggg cgtccgcttt gcgagttgtc tgtccggccg caaacgagcc
     2881 aaagaccgaa caggtcgccg cgacccatcc catcctgtga gccaacccgt gaccgtgcgt
     2941 accgcgacaa accataacga cgcctcccgt cggccgcgat cccgaaccca agcgtatcct
     3001 atcatcggaa gacgagttac cgcaagacaa gcagaaaacg cgcgatgcga tcacacgaga
     3061 cggattgccg ccaacttcag tcaggcacct cggccaagac tgttgagcag gcaacgcagt
     3121 tcggccgggg aagacacggg gacgtgacag gtccggccgt ggcagacatg ggctgaagcc
     3181 ttgccgtcca agagcgtctt gccctccagg gcgggcactt gttgccgtgg aaccgctgga
     3241 tgctcgctgg gatccttgac gaggacaatc cgcgcagggt cgtagccttc gcgggtcacg
     3301 gcgatcaact ctttcgtggc agcgtcacga cggtcgccca cgatgacgat ttcggtgctc
     3361 ggggagcaga gcaggtccac tgcagccaat agtcgttcat ggccaaacgg accgtggtgc
     3421 aggtccccgg ccactgcgcg aactgattgc tcggctttta cacgcagatc atcttcgtca
     3481 aggatcgccg ccagccgcag caggttcatg attgcgaccg agttgcccga tggaatggca
     3541 ccgtcgcgaa agtcctttgt acggacgaaa agcctctcgg catcggatgg ggtgaagtag
     3601 caggccccct cacggtcatc ccagaacagc cgcagcatct catcgttgat ttgagctgct
     3661 cgctcaagcc atcgccagtc aagcgtcgcc tggtgcagct cgagaagcgc ctcaacgagg
     3721 aacgcgtagt cgtcgaggta cgccggcgta tgggccttgc cgttgcgcca ggtccgaagc
     3781 aatcgaccat cagcgatcag attggtcaag atgaattccg ccgctgtcgc cgcagcatcc
     3841 gcgtagcgtg gctcgctgag gacttccgcg gcccgggcca gtgatgtgat caccagagca
     3901 ttccacgcgg tcagggtctt atcgtcgaga gccgggggaa cacgttgcct gcgggccaag
     3961 aggagcttct cgcgggcgat ccggagcatc cggctcaact gtgtctcact gatgccatac
     4021 agccgagcga ctacgtctgg ggctcgttga acattcagga tgttggcgcc ttcccagttg
     4081 ccctgctcgg ttacattgta ataggtgcag aagacctcgc cctcatgttc cccgagaacc
     4141 gccatgattt ccgacttcgt ccagacgtag tacttgccct ctatgccttc gctgtcggcg
     4201 tcccatgctg agtagaaggc tccctcagga gacctcatgt ccgccagcac gtagtcaaga
     4261 atccgggacg cgacgtcggc gtatcgccgg tcgccggtca cctgaaacgc ttccaggtag
     4321 actccgctga ccagcgcctg gtcgtagagc atcttctcga agtgcggcac gagccagctc
     4381 gggtcggtgc tgtaccgagc gatcccgccg gcgagatgat cgtagattcc accgttggcc
     4441 atcttgtcca acgtgagggt cactcgttcc aggagaatcg gatcgggacg tcctcgacgg
     4501 cacgaatgca cgtattcgcg gagcatcagg ctcatggcga tcgacggtgg gaacttgttt
     4561 gtcccgccgc cgctgatgcc gccctgaacg gggtcaaatg cggaagcgag cgcggcggcc
     4621 gccgaactga ccatgtcggc ggtgatggcg gtgtccaggg gtgcgggccg gctgagctgc
     4681 cggacgcctt cgaccaacga tgcggccgca cggcggattt cattgcgatc gtcgtgccag
     4741 agaccggcga ttcgcctcag cagatccgca aacccgattc ttccgtaacg cgatgttggc
     4801 ggatagtagg tcccggcgaa gaaaggcgtc tgatcgtcgg gaaggaggaa cacgctcatc
     4861 ggccagccgc cgttgccttg gttgcacagc aacgtggcct tcatgtagag atcatcgagg
     4921 tccggacgtt gttcacgatc aaccttgatg gaaacgaagt gctcattcag gatggcggcg
     4981 atggccgggt tctcaaaact ctcctgctcc atgacatggc accagtggca cgcggcgtag
     5041 ccgctgctca ggaagatcgg cttgtcttcc cggcgtgctt tttccaaggc ctcggtgccc
     5101 caagggaacc agtccacagg attgcgggcg tgctggagca gatacgggct ggtagagtca
     5161 gccagtcggt ttgcccttgc agcagcctgc gaggcggttc tatcggtcat gaagtctccc
     5221 gcttgttttt cgtatgatcg tgatcattgt agggctcaag accggccgcg acgaccgccg
     5281 aggccaaggg cgtgcggggg cgtggaaagc aggagtgagc tgccggcctt gaggccgggc
     5341 gccgcggaaa gccgcaaatc cctgccgcgt tgctagcgac tctcgggccg gccaaccatc
     5401 agaagcgatt gaccaaacgg taacggcaac agccgttcga aggcatcgat gaaggggacc
     5461 agccggttga agagtcgagc ggattgcagg ttgatttgtt ggcggcgcag gcaccgtcct
     5521 tcccagaacc agccgaagta gccgatcgca ttcatgtagc ggccgtaggt cgggagcagg
     5581 ccggccttgc tgaaaacggc ggccaggctt ttgtggttgt agcgacggca gtgaccaaaa
     5641 gcgcggtcca actcgccgta gatgctcatg tgagccggta ccagaatgac caccctaccg
     5701 tgcggtccgc acaacaggcg catggtgttc aaagcgccga cgtcatcctc aatatgctcc
     5761 aggacgttca tgcaaaggac cgtgtcgaac gcgccggccg gcactgtctc gtttgggcaa
     5821 tcctgtagaa cgccgttgaa aaaccgggtg ttcagatggt cgcggaatcg ctccgtactc
     5881 tgccggagtg acgcggccaa cggttcgatg cccacgacct gccggcggtt gagcaggaac
     5941 tgggtgatgt tccctattcc gcatcccacc tcacacaccg tctcgccgac aaaggggcgg
     6001 actttgttga acaaccagtg gttgtagttg tagagctcgc tgaggacttc aagactggta
     6061 tgcacctcgg cgggcgcacc cgcaatgtcg gccgcggacg acgcgacaga caagggagtc
     6121 gcctccagct cctgcgggca gactgtatct gcggttgctt gcacgctttc ctctatcggc
     6181 gacttggcag cccaggtccg gttatggtgc cgccggcaac ctgcggcctg ttccaaccct
     6241 tccaagatca ctatacattc agccgggcaa gacaaaggtg cagacagtca caccatcatc
     6301 cgataacggc cgggggccac agaagccggc gggccgtccg ataaccatga gcatggcgac
     6361 gttaccagat cgcgatcttc ccctcctgca cgccgagcct gcgatgcgac accctgttag
     6421 caagttggcg gcaacggccc tgaaagccgc ccttccgctg accttatgcc tgatgattgc
     6481 cggcctcttg cgaggatggg gctcgggcgc ctcgttcgag tcctcggatc aggcgatgat
     6541 gccgtacctt gtcaggcaca gcttcggggc tacctgggtt ttcgctcaca gctacggtcc
     6601 cgtaccagtc atcgtacatg catcggtggc aagaatactc tcccaccttg gcattccgat
     6661 ggacgaagcg gtctcgcggc tgccggtcgc ggttctgagc attacgcagg tattcctggc
     6721 gtatctgctg atgcgacggc tgcattgtac acgcaacctg gcaacggcgg cgagcctggt
     6781 atgtgctctc ctgcccccgc tggtcactga cggtcactat gcctggggat atctgacgat
     6841 ctggctgctg ttcggcacca cggcgctctg ggcggcactg gcttacatgg atgacaggca
     6901 gacgtggcat ctgtcggttg cttctttctc gctttttgcg cattgcctga gcaactgctt
     6961 cgttttcggt gtacctctga cgttgctgat aatctgggtg accgtcatcc gacgtgcgaa
     7021 gcaagcacgc cgccgagtca ttaacgatgc gttgttcggg ttcgcgctgc cgtgcctagc
     7081 ggctttcgcc gttgcggtga tgtgccggct tgggaccggc ggcgggcaaa tcggtcggct
     7141 gttagcgaag caacatgccg gcgtgatggg ctggagtccg acaggaatgc tcaaatggct
     7201 ggccatctgg tcaagccagt atggctactt gtttgtggtg ccggccacgg ccggtctgtt
     7261 ccacggtgtg gttttggcca aacggggtga ccgacggggc ctgctggcaa tctgggcata
     7321 tactgccttc ctccctctct tgttgctcag caacccgagc cgaatcggct atcctggtgc
     7381 ctatctcatg gagccggttt tcgccggcgg catgctggcg gtcgtcctgt tcgatcatct
     7441 ctggaatacc ctgtctcgcc gaactctcat cctcaagacc gcctttgtgg cagcgtgcct
     7501 tctgagcatc gcccatctcg gcttcggttc cattgactcc tgcctggccg gcagtcgatt
     7561 tcgacactgg acgggcgtca ccaccggctg ggggagcctg acaccggatt caggcatcaa
     7621 ggccgcgggc tggtatgttc gacagtacgt gccgcctgac gcggtcatca tgccgctgca
     7681 caccaaccgc ggcatggaag ccccggtggc cgagtactac cttggccgtc gcgtcctcgc
     7741 ggcgatggac gtcaatgccg accacctcaa accgctgctt gccgcagttg tcgatcacgt
     7801 cgatcttgtg atcgccgaac cagagtaccg agagatgatt gagaagtctg gtgattttgt
     7861 cgcggtatgc acattcaccc ggaacgggca agccgtgcgg atgatctatg ctcggccggc
     7921 catgcagtta ccagaaatgg tccaggacgt cgcggttgtc aatccccagt acgatcggct
     7981 cttcgcgccc agccgtgttc cgttgccact gcccgcggcg ccggcctaca aggccaagat
     8041 ggacatctat cttcgggccc tgcgccatct caagagcggg cgggcacccg gtccggaatc
     8101 tggaccagcc cagtaaagca ccgtccaagc cgctcgaacg aaacgggaga tagtctcgaa
     8161 atctgtggaa attgacaaga gcggggcatc ctccataagt gatgctgtta tagccagtta
     8221 aaaaaggagg tgccctgatg gctagtagaa ttgatcgacg gggaaaggtc aaggcccgcc
     8281 aacaagcgga cgcgggcgca gcccaggtgg tggtgcggga tcatctggag gagctggccc
     8341 gggacggagc ccggcggatg ctgaccgagg cgttgaacga ggaggtggac gcgtaccttg
     8401 gccgcgggcg gtacgagcgg acaaaggaat accgcggcta ccgcaatggc cgcacgtctc
     8461 ggcggctgac cctgggagcc gggacgatcg agctggcggc cccccgggtg cgcgacatcc
     8521 ccgaagacca ggagccgttc gagtcgaaga tcctccgcaa gcaccagcgt cgcagtgaca
     8581 cgattgacgc gaccttcctg aacctgttcg tggaggggtt ggccacgcgg gacttcgagc
     8641 cggcattacg gctgctggtg ggggcggacg caccgctctc gcccagcacc atcagccggc
     8701 tgagccggca gttcaaggag cagtacgagg ccttcgaggt ccaagacctg agcaaacgta
     8761 ggttcgtgta tatctgggcg gacggcatct acctgaaggc cggcctgggg acggagaaag
     8821 cctgcctgat ggtgctgatc ggggcagata cagagggcgt caagcacctg atcgccctgc
     8881 gggagggtta ccgggagagt gccgagagct ggggcgatct gatccgggat tgccgcaaac
     8941 gcgggctgaa cgagccggcc tgctggattg cggatggggc gttggggttg tgggcggcgg
     9001 tcaatgctca gagtccgcgg tcggcccagc agcgatgcac gaatcataag acgatgaacg
     9061 tgctggacaa gctaccgctc aaagaacgtc cgacctgggt gccgcgcatt cgggccatct
     9121 ggcaggcgga cagcgagaag gcggcccgga agctggcggc ggcggtgatc cacgatctgc
     9181 gggaggcggg ctatgatcgg gcggcggact gcctgggcga tgatctggac cggtgcctga
     9241 cgttttattg cttcccggag gctcactggt cgcacctgcg gaccacgaac ccgatcgagt
     9301 cggtgttcgc gacggttcgg ctgcgaacca atgccgccaa gcgtttcaag aagacccgca
     9361 gcggggtgtg gttgatgcac caggtgctgg aacgtctgag caaacggtgg cgacggttga
     9421 agtcggccca cctctgtccg acggtcccgc tgccggcaga tacgaggaag aaaaccagga
     9481 cccatgcagc atgaggagag tactcggagc gatgccccag agcaatttac acaggagttg
     9541 acactacctc tcgcggccac cgggcgcagg gttgtccgat catacatgta tggagaccca
     9601 ggcgacgttc tctttcagat caacgaattg ccgagatggc aagccgctgt gtacgcgatt
     9661 cgatatcgga tctgcacggg ctggcacaat ttcgaactcg gagacgagaa gacgagtcgc
     9721 aaggaagcgg agaggctgcg agatatcctg gcggccgatt ggttccttga tcggcagaag
     9781 aggagggagt acagacaacg acacccgcag tatgccggtt acacctggcg ccgtttgagg
     9841 gaggaaggcc cgccgggtgg cgcgaaggcc cgctttggcg tgcgatgaca ctggaatggc
     9901 ggcacatcgt accaacccca cattggccga ctgcggtgga tgtgcgagaa gttgcctgcg
     9961 gtctgggcgt gctctttgac aacttcagac gcggcagtgt gtgtttggcc agctcaatct
    10021 gcgtgtgatc gcaaatacgg aaccgcgagg tgttgttgcc cgcagatgga cgcagattca
    10081 cgcagatgtg gacgcggccg gacacggcct tgcgaggtgt tgccatttgc gtctatccca
    10141 tcaatctgcg ggccgagact ccgagcactg ctactgcgac cctcgacacg acgggcgggc
    10201 aatgcctgtg cccacggctc agacgctgct gccccgcccc gactgaccgc tgacaactga
    10261 ctgctgacca ctggccgcca cagcgacccc agtgggaacc ctacggcgcc ctggccaccc
    10321 tggacgtggc cgcctccccc cgccccgaca actccatcgg ccaccaggga ctcttcttct
    10381 acaggttcga tggagccgac agcttgtcgc tgaacgccct cggcctgtac ttcaaccgga
    10441 accggtggta ctcgcccacg cttggccgat tcacgtcgag agatcccaag gcaacgcagt
//
LOCUS       DAOTPW010000203        10439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5975_length_10439_cov_5.553351, whole genome shotgun sequence.
ACCESSION   DAOTPW010000203 DAOTPW010000000
VERSION     DAOTPW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5975_length_10439_cov_5.553351"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="P5316_16305"
     CDS             <1..417
                     /locus_tag="P5316_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRR86574.1"
                     /translation="GNYFVATIFFLVGLILLDVITMPWSGPGQVQMKRKGALAAFILG
                     LVFGIALGPCTFAYMAPILGVAFKVATTSALYGAILLLVYGIGHCSVIVLAGTSTELV
                     QKYLNWNEQSKGAVILKKICGILVLLGGVYLLYTAH"
     gene            437..829
                     /locus_tag="P5316_16310"
     CDS             437..829
                     /locus_tag="P5316_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86575.1"
                     /translation="MKTMFLVVASTAFVMTGGGCAPNSGDQPSEDALIIFHNGTGSAC
                     LAALEWLGTMQSEHPDLVVEEYLTTEFASFLLLNQMKTLYGQSQGVSTTFGYLPIIFY
                     RGQAFSGFNDEVRSALTVLIEATEAPAS"
     gene            855..1229
                     /locus_tag="P5316_16315"
     CDS             855..1229
                     /locus_tag="P5316_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HRR86576.1"
                     /translation="MTDQTGCACGAAPTLIFACSGAADVGEIADRAARRLAREGAGKM
                     YCLAGVGGRVSGILASTQAAEQILAIDGCPLACAKKSLEHAGISRFKYVQLADLGMTK
                     GESPATDDRIAQAATAGIAALS"
     gene            1556..1945
                     /locus_tag="P5316_16320"
     CDS             1556..1945
                     /locus_tag="P5316_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR86577.1"
                     /translation="MKNALIVVGVIVVVVVVAFLKGSGPEPRGSTVPETRPSSGLPRL
                     VELGAGKCQACKAMAPIIEQLRKEYAGKIEVESIDVIEDRERARQIDFNLIPSQVFFD
                     ADGKELWRHEGFMPKDQIVAKFKELGY"
     gene            2641..4212
                     /locus_tag="P5316_16325"
     CDS             2641..4212
                     /locus_tag="P5316_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86578.1"
                     /translation="MGRLLHFLTHANKLWIIPGLLLLVSLVYPGTRELHLLLIRLYCR
                     IPGPQTYVHGQPPGCVIYVIPTVKFNKNTERRILAESNRSVDTFALAHVVNHNYASLP
                     SAETCSGHPLLPWVAFRAADLAYWQAWRSEAHSETQPSIEDDPRPSVAIDEALKIIRM
                     AQANEPENGLLWLAESVVHFAGGSEETAFQAIREAANKKTSNDHVHMLFPYLRDLLVQ
                     HGFPRFDASWSTHLVAWDRPGRLCRPRVREELLDRMILAVENKDDARLMELLSHLIAL
                     YEGCAHQNVSLGKPVGAGERMIAGMISRLGRQMPDEKADYAVRKHAEEAVLYDYLAEY
                     ASTDLSARLRELADADQTGRPGIPGPSGSEANHLLKTAILSSAASEIAFLLMGLGLAT
                     LIAQTSFGMIRKQGMLHQGRVARSWLWPSMLLAAVLMTGAVFNALDLTLQPVGLRVAD
                     REPSPSPTQSNLGTAVAFAAILLLVQFVRTKVPRLYRVSVVVMFCVWLVLVGVAAWLR
                     EDAVTRLAASYATSS"
     gene            4391..5494
                     /locus_tag="P5316_16330"
     CDS             4391..5494
                     /locus_tag="P5316_16330"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HRR86579.1"
                     /translation="MMKCRMLAALMLLVTPAAAQVRKEIRLPDPPGYKILKCDFHIHT
                     VFSDGLVWPTVRVDEAWREGLDAIAITDHIEYRPHKENVVGNHNRPFELAEGRARHHN
                     ILLVRGAEITRDTPPGHFNVLFTQDNQPLDTKDFYEVFDHAAAQKAFIMWNHPGWQGP
                     ERGRWGEEQTRLLEKGRLHAIEICNGDEYYIDAHRMATERGLPMMGSSDIHEPAPTDG
                     QKADGHRTMTLVLAKDKSLPALRDALDAGRTVVWYRNLLIGREPQLKAVFDACLRVES
                     LHPPQAKTTYVKLTNQCDNDIELERIQPGQPRKITVPAQASVMVRLSGPDGQATEKVT
                     YKALNFLTGVDQPLTVEIAIPRPASQPASAAAR"
     gene            5546..8320
                     /locus_tag="P5316_16335"
     CDS             5546..8320
                     /locus_tag="P5316_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86580.1"
                     /translation="MQHALLVFTLTVGTAWAQVDPWQQAQENGRLSRKVVRFSNRYAF
                     GWLTYADPASGLFPRNITRDAFWNAQDCAADNYPFMVLTAFMTDNYYLKQNVRHILQQ
                     EQKLCNRLDSLPDDFLFATQKFREPEPDLDRIIFGAAEYCKDGLMPITEWLGPSEWLD
                     RMQQLIADIWKHAQYDSPAGRIPSTNVEVNGDLLQTMGRLYWMTGDDHYKQWCFRLAD
                     HYLLHDSLIKKDSLRLRDHGCEILGGLSEAYLIAAREDPDRRQRYKPAMYAVLDNILK
                     HGVNEDGFMYNAYNPSTGEVVNKDLSDGWGYVYNAFLTVAMVDGENLYRHAVAKALSN
                     LHKYMTYPWEKGSADDLADPLEGAINLIARIPTDAAVKWIDTRINTLFERQRDDGIAE
                     GWHGDGNLTRTCLMYGLWKTQGITVAPWREDVQLGAVRQADGSLRIWLETGSPWKGYL
                     RFDRPRHKDYFHMPLDYPRINQWPEWFTVNVSGRYRVTREEMTAETVTGKDLLNYPLT
                     RDADKPIKLTITPLKELEVNYDESRVGTYTLPDPLVMRSGEKVTDAAMWKTRRRPEIL
                     ELFRTHVYGRSPGRPSQMTFKTFDNEAKALDGKATRKQVTVFFTGKEDGPSMDILMYL
                     PNAVPKPAPLFLLLNFSGNQGVHSDPAIRLTTSWVPNWSLGIVNNRVTEASRADQESR
                     FPVEQIIKRGYGLATIYYGDIDPDFHDGFKNGVHAAFDPPEGRKPDSWGAIGAWAWGL
                     SRAMDYLETDRDVDAKRVSVLGHSRLGKTALWAGAQDERFAIVISNNSGCGGAAIERR
                     WFGETVEMINDRFPHWFCENFKKYNGREAELPVDAHMLIASIAPRPVYVASAVEDRWA
                     DPKGEFLGAKGADPVYRLLGLEGLPAEEMPPVDQPVMGTIGYHVRTGKHDLTEYDWQR
                     YMDFADKHLK"
     gene            complement(8470..9648)
                     /locus_tag="P5316_16340"
     CDS             complement(8470..9648)
                     /locus_tag="P5316_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRR86581.1"
                     /translation="MRTACLAGRMSHPVLALLMVLPAVGCQQQAREGLDIKGLTRLDP
                     ARGVQDSKTDLLWYDAALLTVEGRGWAATEDFYDRLPAKAKNVVRPEVWGLSKNSAGL
                     CVRFVTDSDRVAARWTTTSKNMAMDHMPATGVSGLDLYVRDNNRWRWVAVGRARSAQT
                     NEVVLAGDVPAGAHEYMLYLPLYNGIKALEIGVVPKAALAQPAPRPAGLDRPMCFYGT
                     SITQGGCASRPGMVYTAILGRWLDRPVINLGFSGNGRMDPELAVLMGEIDASVYVLDT
                     LPNMTKQMVEERLESFVQALRKARPQTPIVLVESVPYQGEAFLTKQRQAVADKNAALR
                     KAYENLKAGGVRNLIYVTNRSLTGTDGEAAVDGVHLTDLGYLRFAETLEPVLRKALRE
                     "
     gene            9873..10403
                     /locus_tag="P5316_16345"
     CDS             9873..10403
                     /locus_tag="P5316_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86582.1"
                     /translation="MSGTYQEWFGGYAMKALCVSSFTIVACTLAGLSFRPAATMAAEP
                     FTLNNGQIYLMLDHRLGGVGDTCKLEVHPFQRYAGNPIIKPDPQWPHGVGQILRDENE
                     QLFKMWYYAGIADTILVGYATSKDGIQWERPSLGLFEVRGSRDNNIVYTGVVPALVRH
                     FPKAAAADQRYVSAPV"
BASE COUNT         2098 a         3220 c         3115 g         2006 t
ORIGIN      
        1 ggcaactact tcgtggcaac cattttcttc ctggtcggcc tgatcctgct ggatgtcatc
       61 accatgccct ggtccggccc cggccaggtt cagatgaagc gtaagggggc cttggcagcc
      121 ttcatcctgg gactggtctt cgggatcgcg ctcggcccgt gtacctttgc gtacatggcc
      181 ccgatcctgg gcgtggcctt caaggtcgcg acgaccagcg ccctgtacgg ggcgatcctg
      241 ctgctggtct acggcatcgg gcactgttcg gtcatcgtgc tggccggcac ctcgaccgaa
      301 ctggtgcaga agtacctgaa ctggaacgag cagtccaagg gtgcggtgat tctgaaaaag
      361 atttgcggta tcctggtgct gctcggcggt gtttatctct tgtacacggc gcactgatct
      421 ggtgggaacg tttgatatga agacgatgtt tcttgtagtg gcctcaacgg catttgtcat
      481 gacgggcgga ggctgcgcgc cgaatagtgg cgaccaaccg agcgaggatg cgcttatcat
      541 cttccacaat ggtaccgggt cggcgtgtct cgcggcgctg gagtggctgg gcacgatgca
      601 atcggagcat ccggacctgg tggtcgaaga atacttgacc accgaattcg ctagtttttt
      661 gttgcttaac cagatgaaga cgctctatgg ccagagccag ggcgtctcga ccacattcgg
      721 ctatctgccc atcatctttt acaggggaca ggcgttttcc ggattcaacg atgaagtgcg
      781 gagtgccctg accgtgctca tcgaagcaac ggaagcgccg gcttcgtaag gcaggagaaa
      841 gcgaggatgg atatatgact gaccaaacgg gttgtgcgtg cggcgcagcg ccgacgctga
      901 tcttcgcctg ttcgggtgcc gccgatgtgg gtgagattgc cgaccgggcg gcgaggcgac
      961 tggctcgcga gggggcaggc aagatgtatt gcctggccgg tgtcggcgga cgggtcagcg
     1021 gcatcctggc cagcacccag gcggccgaac aaatcctggc gatcgacggc tgtcctctcg
     1081 cctgcgcgaa gaaatcactt gagcatgccg ggattagccg gttcaagtac gtccaactcg
     1141 ccgatctcgg catgaccaag ggcgaatcac cggcaacaga tgaccgcatt gcacaagccg
     1201 cgacggccgg gattgccgcg ttgagttgag agacgcttgg ttgttgagcc ggtcggagat
     1261 ggaaatggtt tttctcagac gtttttcgtt tggtcccgtc gccaggttcc tcggctggtg
     1321 ggccagcctg ttcggattca ttgccgcggg tggaagctcg gtctgtccgt gctgcggtac
     1381 gaccggctgc cccatcgggc cggcaacggc cggcgttctg ggtggtgttg tcgcgctgct
     1441 gctgcatgtg cctcgatgga ttgtgaaatc agttcgtcga tgagacaggg tgtgggtatc
     1501 gtgtaagaag gatggggact ctgttccagc cgcgcctgat ggaccgaggt gtgcgatgaa
     1561 gaacgcgctg atcgttgtcg gtgtgatcgt ggtggtcgtg gttgttgcct tcctgaaggg
     1621 ctccgggccg gagcctcgag gctcgacggt tccggagacg aggccttcca gcggcctgcc
     1681 ccggctggtc gaactcgggg cgggcaagtg ccaggcctgc aaggcgatgg ccccgatcat
     1741 cgagcagctt cgcaaggagt acgccggcaa gatcgaggtc gagtcgattg acgtgatcga
     1801 ggaccgggag cgagcccggc agatcgattt caaccttatc ccctcacagg tcttcttcga
     1861 cgccgatgga aaggaactct ggcggcacga gggtttcatg ccgaaggacc agatcgtggc
     1921 gaaattcaag gaactggggt attagtccct tccgtcgcaa ctggtgcgca ttttgcgcgc
     1981 atgtttccgg atcgcagaac gtttgggttg cggcccgcaa aagggcggtc cgccttagcc
     2041 tcttccgtcg cgactggtac gcattctgcg cgcatgtttt ggttgcggcc gaaggtcgcc
     2101 ttggaagata cggagtgtct tcagcaatga ccgaccggtt ggccgttcca cgcactgcgt
     2161 tcaatcgagg cggggctcag cggacaaacc agtttacggg gcaccggctt gctccagtgc
     2221 tcgccgttct catcagaaaa agactcgtgc ggcgcctcgc ggccggtgcg tgtccacatc
     2281 aacaggcgac cgtgcttcaa ttcaatcccc ccgcggaatg gcgcggatgt tccttggtcg
     2341 gctcgataac cagaagtgtc ctgacgccgg ggcggattga gccagcacaa cagcgtcgat
     2401 gagcaatact gcgacaattg cggctcgaca catttcagta atctcctcta tccggacaaa
     2461 gcaggccggt cgagcccgac ttggcgcggc ccgaacgaga gtatcctatc gtatcgtcgc
     2521 cgacaaggag aataggtctt tgccggtaca cgtggtgaat cgcataatga ttcaagaggt
     2581 gttcgtctgg ccgctggcgt ccggcgcggc ccgaagcccg caagcgcgaa ggagtgtacc
     2641 gtggggcgtc tgctgcactt cctcacacac gccaacaagc tctggatcat cccaggttta
     2701 ctgctgctgg tcagtctggt ctatcccgga acgcgagagc tgcacctgtt gctcatccgg
     2761 ctgtattgtc gtatccctgg accgcagaca tatgtccatg gccagccgcc aggatgcgtg
     2821 atatatgtca ttccaacagt caagttcaat aaaaacacag aacgccgtat cctcgcagag
     2881 agcaaccgct cggtcgatac ctttgcgctc gcgcacgttg tgaaccacaa ctacgcgtct
     2941 ctgccatcgg cagagacgtg ctccggacac cccctcttgc cttgggttgc attcagagca
     3001 gccgacttgg catattggca ggcatggcgg tccgaggcac actccgagac acaaccgagt
     3061 attgaggatg acccgaggcc ctcggttgcg atagatgagg cgctcaagat catacgtatg
     3121 gctcaggcga acgagcccga gaatgggctg ctttggctgg ccgaatccgt cgtccatttc
     3181 gccggcggga gtgaggagac cgcctttcaa gcgatccgcg aggccgcgaa caagaaaacg
     3241 tcgaacgatc atgtgcacat gttgttcccc tatctgcgcg acctgctcgt ccagcatggt
     3301 ttccctcggt tcgatgcctc gtggtcgaca cacctggtcg cttgggatcg cccgggacga
     3361 ttgtgcaggc cacgcgtgag ggaggagctt ctcgatcgca tgattttggc ggttgagaac
     3421 aaggacgacg ctcgtcttat ggaattgctg agccatctca tcgctctcta cgagggatgt
     3481 gctcaccaga acgtctcctt gggaaaaccg gttggcgcag gcgagagaat gatcgcaggc
     3541 atgatctcgc gactcggccg ccagatgccc gatgagaaag ccgactatgc cgttcgcaag
     3601 cacgcggagg aggcggtctt atacgactat ctggcagaat atgccagcac agacctctcc
     3661 gcgcgattac gggaactcgc tgacgcggat cagacagggc gaccaggaat acctgggcct
     3721 tcaggctcgg aggcgaatca cctgctcaaa acggcgattc tgagcagcgc cgcctcggag
     3781 atagcctttc tgctcatggg tctcggactg gccacattga ttgcacaaac gtctttcggc
     3841 atgatccgca aacaagggat gctgcatcaa gggcgagtag ccaggtcttg gctctggccg
     3901 tcgatgctct tggctgcggt gctgatgaca ggcgcggtgt tcaacgccct ggatctcact
     3961 ctccagccgg tagggctacg agtcgctgac cgggaaccct cgcctagccc tacgcagtca
     4021 aaccttggta ccgctgtcgc ctttgccgcg attctcctgc tcgtgcagtt tgtcaggaca
     4081 aaggtgccgc ggctctatcg agtcagtgtg gtggtcatgt tctgtgtatg gctggtcctc
     4141 gtgggagtcg ccgcctggct tagagaggat gcagtcacac ggctcgctgc gtcgtacgca
     4201 acctcgtcct gacctgcgaa accccgctcc ccagaaacgc cgcatctcgc ccatccgtca
     4261 cgggaatcgg gggcaagcaa gcagccgccg gcttttgacc ggctctccgc gagagtctag
     4321 aatctgccca ggaaccgtgc cactgtgcgc cggttgtcga ctgtaacctg ttcaagatca
     4381 ggggggctga atgatgaagt gcaggatgct tgcggcgttg atgttgttgg tcacaccggc
     4441 cgctgctcag gtccgcaagg aaatccgctt acccgatccg cccggataca agatcctcaa
     4501 gtgcgacttc cacatacaca ccgtcttctc ggacggcctg gtctggccga cggtgcgcgt
     4561 cgacgaggcc tggcgcgaag ggttggatgc catcgcgatc accgatcaca tcgaatatcg
     4621 gcctcacaag gaaaatgtcg tcggtaacca caaccggccg ttcgaactgg ccgaggggcg
     4681 ggcccggcac cacaacattc tgctggtccg cggtgcggag atcacccgcg acacgccgcc
     4741 ggggcatttc aacgtgctct tcacgcagga caaccagccg ctggacacga aggattttta
     4801 cgaggtcttc gaccacgctg ccgcccagaa agccttcatc atgtggaacc atcccggctg
     4861 gcaagggccg gagcgcggcc gctggggtga ggaacaaacc cgcctgctgg aaaagggccg
     4921 gctccacgcg atcgagatct gcaacggcga cgagtactac atcgacgccc atcgaatggc
     4981 gacggaacgg ggcctgccga tgatgggcag ctcggatatt cacgagcctg ccccaacgga
     5041 cggacagaag gctgacggcc accgtacgat gaccctcgtg ctggcgaagg acaagagcct
     5101 gccggcgctg cgtgatgccc tcgacgccgg ccgaactgtc gtgtggtacc gcaatctgct
     5161 catcggccgc gaaccgcagt tgaaagccgt ctttgacgcc tgcctccgtg tcgaatcgct
     5221 gcacccgcca caagccaaga ccacctacgt gaaactgacc aatcaatgcg acaacgacat
     5281 tgagctggag cgaattcagc ccggccagcc tcgcaagatc acagtaccgg cccaggcatc
     5341 agtgatggtc agactctccg gaccggatgg gcaggccacc gagaaggtga cctacaaggc
     5401 attgaatttc ctgacgggag tcgatcagcc attaaccgtg gaaattgcga tccccaggcc
     5461 cgcttcgcaa cccgcctccg ctgcggcccg ttgaacccac tgtcaggcac gctctcgcga
     5521 ggcaccagca agggaggtaa cgacgatgca acacgcactt ctggttttca cgctgactgt
     5581 cgggacggca tgggcgcagg ttgacccatg gcaacaggcc caggagaacg gccggctcag
     5641 ccggaaggta gtgaggttct ccaaccgcta cgccttcggc tggctgacct acgccgaccc
     5701 ggccagcggg ctgtttccac gcaacatcac ccgcgacgcc ttctggaatg cccaggactg
     5761 cgccgcggac aactacccgt tcatggtcct gacggcgttc atgaccgaca actactatct
     5821 caagcagaac gtccgccaca tcctgcagca ggaacagaag ctgtgcaacc ggttggactc
     5881 gctgccggat gatttcctct tcgccacgca gaagttccgc gagcccgagc ccgatctcga
     5941 ccgcatcatt ttcggggccg ccgaatactg taaggacggg ctgatgccga tcaccgagtg
     6001 gctcggccca agcgagtggc tcgaccggat gcaacagttg atcgccgaca tctggaaaca
     6061 tgcccagtac gactcgccgg cggggaggat cccctccacc aacgtcgagg tcaacggcga
     6121 cctgcttcag accatgggac gcctgtactg gatgaccggc gacgaccact acaagcaatg
     6181 gtgcttccgg ctggctgatc actacctgct gcacgacagt ctgatcaaga aagacagcct
     6241 ccggctgcgc gatcacggtt gcgagatcct cggcggcttg tccgaggcct acctgatcgc
     6301 agcccgcgag gaccccgacc gtcgacagcg atacaagccg gcgatgtacg ccgttttgga
     6361 caacatcctg aaacacggcg tcaacgaaga cggcttcatg tacaacgcct acaatccaag
     6421 cacgggcgag gtcgtcaaca aggacctcag cgacggctgg ggttatgtct acaatgcgtt
     6481 tctgaccgtc gccatggtcg acggcgagaa cctctaccgg cacgccgtcg ccaaagcgct
     6541 gtcgaatctg cataagtaca tgacctaccc ctgggaaaag ggtagcgcgg acgatctggc
     6601 cgatccgctt gaaggagcga tcaacctgat cgccagaatc cccaccgatg cggccgtaaa
     6661 gtggatcgat acgagaatca acacgctctt cgagcggcag cgcgacgacg gcatcgccga
     6721 aggctggcac ggcgacggca acctcacccg tacgtgtctg atgtacggtc tctggaaaac
     6781 acagggcatc accgtcgcgc cttggcgcga ggacgttcag ctcggtgcgg tccgccaagc
     6841 cgatggttcg ctccgcattt ggctcgagac cggttctccc tggaaggggt atctgcggtt
     6901 cgaccggccg cgccacaagg attacttcca catgccgctg gattatccac gaatcaacca
     6961 gtggcccgag tggttcacgg tcaacgtatc cggtcgatac agggtgaccc gcgaagaaat
     7021 gaccgcggag acagttaccg gcaaggacct gctgaactac ccgctgacac gcgatgccga
     7081 caagccgatc aaacttacta taacacccct caaagagctt gaggttaatt acgacgagtc
     7141 cagggtcggc acgtacaccc tgcccgatcc gctggtcatg cgcagcggcg agaaggtaac
     7201 tgacgccgca atgtggaaga ccaggcgacg acccgagata ctcgaactct tccgaacgca
     7261 cgtctacggc cggtcgcccg gcagaccgag tcagatgacg ttcaagacct tcgataacga
     7321 agccaaggcc ctcgacggca aagcaacgcg caagcaagtg accgtctttt tcaccggcaa
     7381 ggaagacggt ccctcgatgg acatcctgat gtatctcccg aacgcggtac ccaaaccggc
     7441 ccctctgttc ctcctcctga acttctcggg caaccagggt gttcactcgg atccggcgat
     7501 ccgactcacg actagttggg taccgaactg gagcctcggt atcgtcaaca accgggtgac
     7561 cgaggcctcg cgagcggacc aggaatcacg gtttccggtc gaacagatca tcaaacgagg
     7621 ttacggtctg gccacgatct actacggcga catcgaccct gactttcatg acggcttcaa
     7681 gaacggcgtt cacgcggcat ttgacccacc tgaagggcgc aagccggact catggggtgc
     7741 gatcggcgcc tgggcctggg gtctgagccg ggcgatggac tacctggaga ccgatcgcga
     7801 tgtcgatgcg aaacgtgtgt cggtgctcgg ccactcgcga ctgggcaaga ctgccctgtg
     7861 ggccggggca caagacgagc ggttcgcgat cgtcatttcc aacaactcgg gctgcggggg
     7921 cgcggccatc gagcggcgct ggttcggcga gacggtcgag atgatcaacg atcgctttcc
     7981 ccactggttc tgcgagaact tcaagaaata caacgggcgt gaggccgagc tgccggttga
     8041 tgcccacatg ctcatcgcgt cgatcgcccc aagacccgtc tacgtggcca gcgccgtcga
     8101 agatcgctgg gccgatccga aaggcgaatt cctgggggcc aagggcgccg atccggtgta
     8161 ccgattgctg ggcttggagg gcctgccagc agaagagatg ccgccggtgg accagccggt
     8221 gatgggcacc atcggctatc acgtccgcac cggcaagcac gacctgaccg aatacgactg
     8281 gcagcggtac atggatttcg ctgacaagca cctgaagtag ggcacggtcg gagccgccaa
     8341 aggcggcgca gacctgccgg ttcgctgttg atcgtgcttt ctgagaaccc ggcggccaac
     8401 gaaacggcag gtctcggtcg ccttggcgac ctcgacgctg ccctacctct tggatcttcc
     8461 gctatttgct cactcccgga gcgcctttct caacactggt tcgagggttt ccgcgaaccg
     8521 caggtagccg agatccgtca gatgcacgcc gtccacagcg gcttcaccgt cggtgccggt
     8581 caggctccga ttggtcacgt aaatcaggtt cctcacaccg cccgccttga ggttctcata
     8641 cgcctttcgc agggccgcgt tcttgtctgc gaccgcctgc cgctgcttgg tcaggaacgc
     8701 ctcgccctgg tacggcacgc tttcgaccag cacaatgggc gtctgtggcc tggccttgcg
     8761 aagtgcttgc acgaacgact ccagccgctc ctcaaccatc tgcttggtca tgttcggcaa
     8821 agtgtcgaga acgtataccg acgcgtcgat ctcacccatc agcacggcca actccgggtc
     8881 catccgcccg ttgcctgaga agccgaggtt gatcaccggg cggtccagcc atctccccag
     8941 gatggcggtg tagaccatcc ccggccgcga ggcgcatccg ccctgggtaa tcgaggttcc
     9001 atagaagcac atcggccggt caagcccggc tgggcgcgga gcgggttgag ccagagcggc
     9061 cttgggaacc acgccgatct ccagcgcctt gattccgttg tacagcggca ggtacagcat
     9121 gtactcgtgt gccccggccg gaacgtcccc ggccagcacg acctcattcg tctgtgcgct
     9181 tcgcgctcga cccaccgcaa cccatcgcca ccggttatta tccctgacgt acaggtccaa
     9241 cccgctcact ccggtggccg gcatgtgatc catcgccatg ttcttcgacg tcgtcgtcca
     9301 tcgggcggcg acccgatccg aatcggtcac gaaacgaaca cacagcccgg ccgagttctt
     9361 gctcaggccc cacacttcgg ggcgaaccac attcttcgcc ttggccggca aacggtcata
     9421 gaagtcctca gtcgccgccc aaccacgacc ttcgacggta agcagcgctg cgtcatacca
     9481 aagcagatcg gtcttggagt cctggacacc tctggccgga tcgagcctcg tcaacccctt
     9541 gatgtccaaa ccctcccggg cttgctgctg acaacccacg gccggcagca ccatcaacag
     9601 cgcaaggact ggatgactca ttcttccggc aagacaagca gttctcatca ggtcgcctcc
     9661 tcatgacctc tctggcaaac accccttcgt atcgtgtggg gtcctccttg tcaaaaccct
     9721 caacgagccc gtctctcccg aacaagtcgc catacacaag ggataccaag cagaacattc
     9781 tgcttcctca caatgccaca accacttcct tgttttcttt cgtcgcgccg gcccatttca
     9841 gaccgagcag tcccacgcta gaatcctcct tcatgagcgg aacttaccaa gagtggttcg
     9901 gaggctatgc catgaaagcc ctgtgcgtct catcgtttac cattgtcgcc tgcactcttg
     9961 ccggactgtc gttccggcct gccgcaacca tggcggctga accgttcacc ctgaacaacg
    10021 ggcagatata cctcatgctc gatcaccggc tgggcggagt aggagacacc tgcaaattgg
    10081 aggtccaccc gttccaacgg tacgccggca acccgatcat caagccggac ccccagtggc
    10141 cgcacggcgt cgggcagatc ctccgggacg aaaacgaaca actgttcaag atgtggtact
    10201 acgccgggat cgccgacacc atcctggtag gctacgcgac cagcaaagat ggcatccaat
    10261 gggaaagacc gtcgctgggc ctgttcgagg ttcgcggcag ccgcgacaac aacatcgtct
    10321 acacgggcgt cgtgcctgct ttggtcaggc atttcccgaa ggccgcggca gcagaccagc
    10381 gctacgtgtc ggcgccggtc tgaacatgct gtaaatccgc cggtttgatg atgcggtga
//
LOCUS       DAOTPW010000204        10437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5979_length_10437_cov_6.705355, whole genome shotgun sequence.
ACCESSION   DAOTPW010000204 DAOTPW010000000
VERSION     DAOTPW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5979_length_10437_cov_6.705355"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..488
                     /locus_tag="P5316_16350"
     CDS             153..488
                     /locus_tag="P5316_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HRR86583.1"
                     /translation="MATTQFIHDGNSIDYTPGANVSAGDVVVQNDLIGIAKRDIAANA
                     LGALAVTGVFDVPKATGAGTAIAAGAKVYWNAAAQQATTTATGNKYLGKPVRAAADAD
                     ATVRVRLEQ"
     gene            492..845
                     /locus_tag="P5316_16355"
     CDS             492..845
                     /locus_tag="P5316_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86584.1"
                     /translation="MADLLRQGSQWLEQMRTAHCSSPVEYRRPPDAYTVTATYGKTGF
                     EVADESGLTINAHVWDFLILADELGFDPEPGDVIAANGRRYEVMNLGGEGCWRWSDPY
                     RQTYRIHTKDIGADT"
     gene            842..1069
                     /locus_tag="P5316_16360"
     CDS             842..1069
                     /locus_tag="P5316_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86585.1"
                     /translation="MSECSQFEQCQNQFESIHGKLDRLDEAIRGNGKPGIQLRLDRLE
                     QDAKRQSKLIWLIVGAVITTLASGVVAWITG"
     gene            1073..1510
                     /locus_tag="P5316_16365"
     CDS             1073..1510
                     /locus_tag="P5316_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86586.1"
                     /translation="MSLVIDIADAVTAELNAAPAGTFVPAIVAVRRVLPEFELADLAE
                     LKVSVVPKSVVITGSTRATSQYEIAVDIGVQKKLGKDLDGEVAALGTLMDQIADYLRR
                     RSLSAAPFAAWIGIANEPVYAPEHLAEQRVFTSVLTVTYRAMK"
     gene            1529..1783
                     /locus_tag="P5316_16370"
     CDS             1529..1783
                     /locus_tag="P5316_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86587.1"
                     /translation="MNNTIMRKLVLTANYQPLAAEKVVGSFVISAPPSNANDAIFLGD
                     EGADVPWVPGEWHSFRNVDLAAIQVKGTPGDIVTVVGGTW"
     gene            1783..2358
                     /locus_tag="P5316_16375"
     CDS             1783..2358
                     /locus_tag="P5316_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86588.1"
                     /translation="MPYIQEPLPVVSTPPMREWTLLQLPYDGSITEIKHINMRNLQEM
                     YCDYNQMTTLPWDELQNLYYLGIYNCAFITLELWQMPNLYSCYAGDNYNLESVDAHDM
                     PNLGDLDIYYCQSLTNLDVSGCSNLRYIYAYGCAFDEAMVDQLLTDLVANGTTNGYLQ
                     VNGPYNSPPSNPDGVALKNILISRGWSVYTS"
     gene            2367..2630
                     /locus_tag="P5316_16380"
     CDS             2367..2630
                     /locus_tag="P5316_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86589.1"
                     /translation="MKEVQATTVQVKISDGNEDKFVLVHDGQQVIELAEPGGKVGTPP
                     GNTMLVGTKEELEAEVARLKLKAKKPRRPADRMPPTPTPQREP"
     gene            2630..3067
                     /locus_tag="P5316_16385"
     CDS             2630..3067
                     /locus_tag="P5316_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86590.1"
                     /translation="MIGMDFKSTLGTFFDRKAVMSKVDSASRKVLSKFGAFVRRSAKS
                     SIRKRKKPSPPGSPPSSHTGLLKKFIFFGYDLDARSVVIGPTRLNQKGRGDAPPLLEY
                     GGKATLVRRGKKKRTTYKARPYMGPAFEKEKPQLPAMWRDSVR"
     gene            3080..3517
                     /locus_tag="P5316_16390"
     CDS             3080..3517
                     /locus_tag="P5316_16390"
                     /inference="COORDINATES: protein motif:HMM:NF017961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HRR86591.1"
                     /translation="MSQEFLLGMNAKIYQGAAGAALNTLTEMANVKDVTLNLEAGEAD
                     VTTRANQGWRATAPTLRECTAEFEMLWKPGDAGFDAVKTAFLSSGTIRLAVLTGDRTA
                     SGTEGPLGDFSITNFSRNEPLEEGVTVSVTAKLAVFDSWVEVA"
     gene            3517..3987
                     /locus_tag="P5316_16395"
     CDS             3517..3987
                     /locus_tag="P5316_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86592.1"
                     /translation="MKTFTDAAGRTWTLTLTLGTAMKVKAKLDIDLLQPEAGDPPLLT
                     RLGTDEMLLGEVLCAMLEGQFETHKVTDDDVRAGFDGQTLLAAQKAFYEELIGFFRSR
                     GRNDRAKAVAKQMAMIDAAVTAVETRIDALDIDATIRGAMSGASPGPSASTPAR"
     gene            4008..4232
                     /locus_tag="P5316_16400"
     CDS             4008..4232
                     /locus_tag="P5316_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86593.1"
                     /translation="MAEGLGRERWAHTSLICTLIANANRDPKKGRPFKPSDFDPYARA
                     DRRSARVADKQSLAILREALEAQKGNEHGR"
     gene            4222..4605
                     /locus_tag="P5316_16405"
     CDS             4222..4605
                     /locus_tag="P5316_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86594.1"
                     /translation="MDGSAILNGIWSFFNSSIGFAVIWAAMVGFFIFLASKFNPLQEK
                     WKAWEGSIITGIKLAEKQIADDTPNRGLAKLDAALRFVLNAYAEANNGKQPSDALVEQ
                     IKQGIQIKHNELDRFGGLESKKEAA"
     gene            4605..4760
                     /locus_tag="P5316_16410"
     CDS             4605..4760
                     /locus_tag="P5316_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86595.1"
                     /translation="MSWLIAVLTAFFQALLPWVAKKSRPTAEDGNPDQQTKDKLRAKV
                     RKHWSQP"
     gene            4757..4993
                     /locus_tag="P5316_16415"
     CDS             4757..4993
                     /locus_tag="P5316_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86596.1"
                     /translation="MKLLKHLIPFLLPILVLTGCGGGSPFTRTIYVPHGTPVRLRETV
                     QDVKVWVKDADGTPVAGKMDLPEGWYALPVDDQE"
     gene            4999..7017
                     /locus_tag="P5316_16420"
     CDS             4999..7017
                     /locus_tag="P5316_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86597.1"
                     /translation="MANAQGIRAGRAFVELFADDSKLVRGLRRAEKKLKAFGDSVRNF
                     GLKLAGLGTAMLAPLVASAKLFSGYGDQVAKMAKRTGVSVETLSELQFVASQTGTEFE
                     SLEMGFRKMQRSIYDAERGLSTAVDALADLNLTAADFKGLSPEQQFKLLADRISQVED
                     PTRKAAIAMSLFGRTGTNLLPMFASGAKGIEILQAEARRLGLTMSAEDAKAAEDFTDA
                     LDRLWKVVKMGIFHVGAALAPVLQQVADTITNLGVRISAWVQQNQQVIVTVLKVVVGV
                     IAVGVAIAVLGTIISALGTILGGLITVITTVVAVLQVLGAVIAFLVSPIGLVIAAVAA
                     LAGYLIYATGAGGKALAWLGGRFNVLKEDALSAYQGIADALAAGDIGLAVKVLWLTVK
                     MEWTRGVNFLEKAWLNFRNFFIKIGYDAWHGLLAVAEIVWHALEVGWIETTAFFSKLW
                     TGFTSFFAKTWENIKAGAQKAWNWIKSLFDDSVDLEAENKLVEEQKQAAIARIEDEQK
                     RKTAQREAERESERRRAAAIHEATLGEIGRENMEKHRALDTEYAQRMAENEADLAKAR
                     QEWRDAIDEARKKREAKEAEGGPDGLEGPDDIINKARDALAGLGDIGDLVQQEAAKIG
                     VKGTFNAAAVQGLAAGDAADRTAKASEETAKNTKKLVQAAQTGGLTFA"
     gene            7042..7869
                     /locus_tag="P5316_16425"
     CDS             7042..7869
                     /locus_tag="P5316_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86598.1"
                     /translation="MPIVCTENIDSRQITETQSAELLYTISGTADEAAAIASLKATAP
                     TVLHNMKRQPVTVEPVHVDTTNPDKCIWTGTVNYAPFEYQEPPETGDSSFSFDTGGGT
                     QHITQSLSTIGRYAASGTAPDFKGAIGVTHDNVEGVDITVPVYNFSETHYLSAATVTT
                     AYKGTLFQLTGKVNSGSFRGLAAGECLFLGASGSRRGTGPDDDWEITFRFAGSPNRTG
                     ITVGPISGIAKKGWEYLWVRYADAEDTGSNTLVKQPIAAYVEKVYEDGNFAALGIGS"
     gene            7866..8711
                     /locus_tag="P5316_16430"
     CDS             7866..8711
                     /locus_tag="P5316_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86599.1"
                     /translation="MSDGLKKVQSGQPLTIPASAYNAFIDAALDFRQRTAHLGQGAQP
                     SFSQASIVLVRNDSGSNQTRMAVLGVDTPIIDPSANEEEFKNRVALSCVAPAADTHEG
                     KFVILAEPIATGKIGRAYAAGVCPVKIDVPDEDHEWRYAEIADGVTANLKVSMQGSAG
                     VLWRAGGTGVQWSVVRLGKPTPMHVFPVDLTQYGGEQGDEENPATWTYDVLDVVTGET
                     LESGVDPVAAPHKWQRPSIGQMIAATFGYAHYQGDGAGGLELVLGWINEMVDQEACET
                     ASSTE"
     gene            8722..9345
                     /locus_tag="P5316_16435"
     CDS             8722..9345
                     /locus_tag="P5316_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86600.1"
                     /translation="MGAPGKSVVVEGGKRGVLLGGKSAVYNAEEICPACCIEFTQQWS
                     FTDSGFIDGGQDGAFRAYDNPADVPASPWSILNNGLGLRLDWEDDQNCRSHNPYTQYA
                     TATCQITVPKAMIMTVNWSGMGETQDPNYELMSLYVNGGLVGSAHAPGGGQGCAGMGP
                     VISNPPPPQQVLLNPGPHTLFIDATTNDPLYHFGAWYRFDLTFELAP"
     gene            9356..9697
                     /locus_tag="P5316_16440"
     CDS             9356..9697
                     /locus_tag="P5316_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86601.1"
                     /translation="MSETLTPKQKKPCTNCPPVVIPRRAYTPTRPSCVECVEKHLGAA
                     LVLLTETREGYAYRLRAIGHLFEAEDESQEWPELHAAIREARKAYQADGTLPEWETLA
                     ETVVRTAAVKT"
     gene            complement(9885..10319)
                     /locus_tag="P5316_16445"
     CDS             complement(9885..10319)
                     /locus_tag="P5316_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86602.1"
                     /translation="MHFTRGQRTRKVIQEGPPPELDATPEGTVPRISRLMALAIRFDR
                     LIKAGEIADQADLARLGHVTRARVTQIMNLLQLAPDIQEDILFLPRTFRGRDPIREIM
                     VRSIAAEPDWRKQRRLWKCLTFDQGVETVPVGEGVAPDRPLC"
BASE COUNT         2065 a         3307 c         3418 g         1647 t
ORIGIN      
        1 acgttctggg catccagttc cgtgggtact tcgacttcgg cgtcgccctc caggattacc
       61 ggggcggcgt gaagatgaag ggcgaggcgt aagcctcccc cggagtccct tcggcgacgt
      121 gaaactttca gaccaacaag gagcaatgac tcatggcaac gactcagttc attcatgacg
      181 gcaacagcat cgactacacg cccggcgcga acgtcagcgc cggggacgtg gtcgtgcaga
      241 acgatctcat cggcatcgcc aagcgggaca tcgctgccaa cgccctcggg gcgctggccg
      301 tgacgggcgt gttcgacgtg cccaaggcga ccggcgcagg caccgccatc gcggcgggcg
      361 cgaaggtgta ctggaacgcc gccgcccagc aggcgaccac caccgccacg ggcaacaagt
      421 acctcggcaa gccggttcgc gcggccgccg atgccgacgc gacggttcgc gtccgcctgg
      481 agcagtaggc gatggccgac cttctccgcc aaggctcgca gtggctggag cagatgcgca
      541 cggcgcactg ctccagcccg gtcgagtacc gcagaccgcc tgacgcttac accgtcacag
      601 cgacctacgg caagaccggc ttcgaggtcg cagacgagtc gggcctgacg atcaacgccc
      661 acgtctggga cttcctgatc ctggccgacg aactcggctt tgacccggaa ccaggcgacg
      721 tgatcgcggc caacgggcgg agatacgagg tcatgaatct gggcggcgaa ggttgctggc
      781 gatggagcga tccgtatcgg caaacctacc gcatccacac caaggacatc ggagcagaca
      841 cgtgagcgag tgcagccagt ttgagcagtg ccagaaccag ttcgagtcca tccacggcaa
      901 gttggatcga ctcgacgagg cgattcgcgg caacggcaag cccgggattc agcttcggct
      961 ggatcgcttg gagcaggacg cgaagcgcca atcgaaactc atctggctca tcgtcggcgc
     1021 ggtcatcacc acactggcat cgggcgtcgt cgcctggatc acggggtaac gcatgagtct
     1081 ggtcatcgac atcgcagatg cagtgacagc ggaactgaac gcggccccgg cgggcacgtt
     1141 cgtaccggcc atcgtcgcag tacggcgcgt gctgccggag ttcgagttgg ctgacctggc
     1201 ggaactgaag gtctcggtgg tgcccaagag cgtggtgatc accggttcca cgcgggcgac
     1261 cagccagtac gaaatcgcag tggacattgg tgtgcagaag aagctgggca aagacctcga
     1321 cggcgaggtc gcggcactgg ggacattgat ggatcagatt gccgactacc tgcgtcgccg
     1381 ttcgctcagt gctgcaccgt tcgccgcatg gatcggcatc gccaacgagc cggtctacgc
     1441 accggagcat ctggccgagc agcgagtgtt caccagcgtg ctgactgtga cctaccgggc
     1501 catgaagtga gccaatggag cccaagacat gaacaacacc atcatgcgca aactcgtcct
     1561 gaccgccaac taccagccgc tggctgcgga gaaggtcgtc ggctcattcg ttatcagcgc
     1621 ccctccgagc aacgccaacg atgccatctt tctcggtgat gagggagccg acgtgccgtg
     1681 ggttccaggc gagtggcact cgttccgcaa cgtggacctc gccgcgattc aggtcaaagg
     1741 tacgcccggc gacatcgtga ccgtggttgg agggacgtgg taatgcctta catccaggaa
     1801 ccattgccgg ttgtcagcac gccgcccatg cgggaatgga ccctgctgca attgccctac
     1861 gacggcagca tcacggagat caagcacatc aacatgcgca accttcagga gatgtactgc
     1921 gactacaacc agatgaccac gctgccgtgg gatgaattgc agaacctgta ctacttgggc
     1981 atctacaact gcgcgttcat cacactcgaa ctgtggcaga tgccgaactt gtactcgtgc
     2041 tacgccggtg acaactacaa cctcgagtcg gtcgacgccc acgacatgcc caacctcggc
     2101 gacctggaca tctattactg ccagtcgctg accaacctgg acgtttcggg atgctcgaat
     2161 ctgcgctaca tctacgccta cggctgtgca ttcgacgagg cgatggttga ccaactactc
     2221 actgaccttg tcgccaacgg caccaccaat ggctacctcc aagtgaacgg gccgtacaac
     2281 tccccgcctt ccaaccccga tggcgtggcg ctcaagaaca tcctgatcag caggggctgg
     2341 tcggtctaca caagctgagg attgccatga aagaagtcca agccaccact gtgcaggtga
     2401 agatctccga cggcaacgag gacaagttcg tgttggtcca cgacggccaa caagtgatcg
     2461 aactggcgga accaggcggc aaagtcggca ccccgccagg caacacgatg ctcgtcggca
     2521 ccaaggaaga actggaagcg gaggtcgcaa ggctgaagct gaaagccaag aagccgcgac
     2581 gacccgctga tcgtatgccg cccacgccaa ccccgcagcg agaaccgtga tgatcggcat
     2641 ggacttcaag agcacgctcg gcacgttctt cgaccggaag gccgtgatgt ccaaggtgga
     2701 ctccgcctcg cggaaggtgc tctcgaagtt cggggcgttc gtgcggcgat cggccaagag
     2761 cagcatccgc aaacggaaga agccatcgcc acccggatcg ccgccaagct cgcacacggg
     2821 actactgaag aagttcatct tcttcggtta cgaccttgac gcccgcagcg tggtgatcgg
     2881 gccgacgcgg ctgaatcaga agggacgtgg cgatgcgccg ccactgctgg agtacggcgg
     2941 caaggccacg ctggtcaggc gaggcaagaa gaagcgcacg acgtacaagg cccggcccta
     3001 catgggaccg gcattcgaga aagagaaacc ccagctgccc gccatgtggc gagacagcgt
     3061 tcgataagga gatcgaagca tgtcgcaaga attcctgttg ggcatgaacg ccaagattta
     3121 ccagggggcg gcgggcgcag ccctcaacac gctcaccgag atggccaacg tcaaggacgt
     3181 gacgttgaac ctcgaggcgg gcgaggccga tgtcaccacc cgcgccaacc agggctggcg
     3241 ggcgaccgcg ccgacgttgc gcgaatgcac cgccgagttc gagatgctct ggaagccggg
     3301 tgacgccggg ttcgacgcgg tgaagaccgc gttcctctcg tcgggcacga tccgtctggc
     3361 cgtgctgacc ggcgaccgca ccgcctcggg caccgagggg ccgctgggcg acttcagcat
     3421 cacgaacttc agccgcaacg agccgctgga agagggtgtg acggtcagcg tgaccgccaa
     3481 gctcgccgtg ttcgattcct gggtggaggt ggcctgatga agacgttcac tgatgcagcc
     3541 ggacggacct ggacgctcac cttgaccctc ggcacggcca tgaaagtcaa ggcgaagctc
     3601 gacatcgatc tgctccaacc cgaggcgggc gatccgccgc tgctgacgcg gctcggcacc
     3661 gacgagatgc tcctgggcga ggtgctctgc gccatgctcg aggggcagtt cgagacgcac
     3721 aaggtcaccg acgacgacgt gcgggccggt ttcgacgggc agacgctgct ggcggcgcag
     3781 aaggccttct acgaggaact gatcggtttt ttccggtcgc gcggccgcaa cgaccgggcc
     3841 aaggcggtcg ccaagcagat ggccatgatc gacgcggcgg tgacggcggt ggagacgcga
     3901 atcgacgcgc tggacatcga cgcgacgatc cgtggggcga tgtctggcgc atcgccgggt
     3961 ccatcggcgt cgaccccggc ccgctgacgc tgcggcagct gctgtggatg gccgaagggt
     4021 tgggccgcga gcggtgggcg catacatcgc tgatctgcac cctgatcgcc aacgccaacc
     4081 gcgacccgaa gaagggccgc cccttcaagc cgtcggactt cgatccttac gctagggcag
     4141 accggcgatc cgcgcgggtc gccgacaagc agtcactcgc aatcctcaga gaggcccttg
     4201 aggcccagaa aggcaacgaa catggacggt agcgcaatcc tcaacggaat ctggagcttc
     4261 ttcaactcga gcatcggctt cgcagtcatc tgggcggcga tggtcggctt cttcatcttc
     4321 ctggccagca agttcaaccc gctccaggag aagtggaagg cctgggaggg cagcatcatc
     4381 accggcatca agctggccga gaagcagatc gccgacgaca cgcccaacag gggcctggcc
     4441 aaactcgacg cggccctgcg gttcgtattg aacgcctacg ccgaggccaa caacggcaag
     4501 cagccctccg acgcgctggt cgagcagatc aagcaaggca tccagatcaa gcacaacgaa
     4561 ctggaccgct tcggcgggct ggagagcaag aaggaggcgg cgtgatgagt tggttgatcg
     4621 ctgtgctgac ggcgttcttc caagcccttc tgccgtgggt cgccaagaag tcgcggccca
     4681 cggcggaaga cggcaacccg gaccagcaga ccaaggacaa gttgcgggcc aaggtccgca
     4741 agcactggag ccagccatga aactcctcaa gcacctgatc ccgttcctcc tgcccatcct
     4801 cgttctgacc ggctgcggcg gcggatcgcc gttcacgcgg acgatctatg tcccgcacgg
     4861 cacgcccgtg cgcctgcgcg aaaccgtcca ggacgtgaag gtctgggtca aggatgccga
     4921 cggcacaccc gtcgctggca agatggacct gcccgagggc tggtacgcct tgcccgtgga
     4981 tgaccaggag taacgcacgt ggccaacgcg cagggaatcc gagccggtcg agcgttcgtc
     5041 gagctgttcg ccgacgacag caagctcgtg cgcgggctgc gccgtgccga gaagaagctc
     5101 aaggccttcg gtgattcggt ccgcaacttc ggcctgaaac tcgccggtct gggaacggcc
     5161 atgctcgcgc cgctcgtggc gtcggccaaa ctgttcagcg gatacgggga ccaggtcgcc
     5221 aagatggcca agcggacggg cgtgagcgtg gagacgctca gcgagcttca gttcgtcgcc
     5281 agtcagaccg gcacggagtt cgagtcgctg gagatgggct tccgcaagat gcagcggagc
     5341 atctacgacg ccgagcgcgg cctgtccacg gcggtcgatg cgctggctga tctgaacctg
     5401 accgccgccg acttcaaagg actctccccc gagcagcagt tcaagctgct ggccgaccgc
     5461 atttcgcagg tggaagaccc cacccgcaaa gcggcaatcg caatgagcct gttcggtcgc
     5521 accggcacga acctgctgcc gatgttcgcc agcggggcca agggcatcga gattctgcag
     5581 gccgaagcgc ggcgactggg gctgaccatg tccgccgagg acgccaaggc cgccgaggac
     5641 ttcaccgatg ccctcgaccg tctgtggaag gtggtcaaga tgggcatctt tcacgtcggt
     5701 gcggcgctgg ccccggtgct gcagcaggtc gccgacacga tcaccaacct gggcgtgcgc
     5761 atctcggcgt gggtgcagca gaaccagcaa gtcattgtca ccgtgctcaa ggtcgtggtc
     5821 ggcgtcatcg ccgtcggcgt ggccatcgcg gttctgggaa cgatcatctc cgcgttgggc
     5881 acgatccttg gcggcttgat caccgtcatc accaccgtgg ttgccgtgct gcaggtgttg
     5941 ggcgcggtga ttgcgttcct ggtctcgccc atcggcctgg tcatcgcggc cgtcgcggcg
     6001 ctggccgggt atttgatcta cgcgacgggc gcgggcggta aggcgctcgc gtggctgggt
     6061 gggcgattca atgtgctcaa ggaagacgcg ctctccgcct accagggcat cgccgacgcg
     6121 ctggcggcgg gcgacatcgg tttggcggtg aaggttctgt ggctcacggt caagatggag
     6181 tggacgcgcg gggtgaactt cctcgaaaag gcgtggctga acttccgcaa cttcttcatc
     6241 aagatcggct atgacgcctg gcacggcctg ctggccgtcg cggagatcgt ctggcacgcc
     6301 ctggaggtcg gctggatcga gacgacggct ttcttctcga agctctggac cggcttcacc
     6361 agcttcttcg ccaagacctg ggagaacatc aaagccgggg cgcagaaggc gtggaactgg
     6421 atcaagtcgc tgttcgacga ttcggtcgat ctggaagccg agaacaaact cgtcgaggag
     6481 cagaagcaag ccgccatcgc ccgcatcgag gatgagcaga agcgcaagac cgcccagcgt
     6541 gaagcggagc gggagtccga gcgccgacgc gcggccgcga tccacgaggc gacgttgggt
     6601 gagatcggcc gcgagaacat ggagaagcat cgggcgctgg acaccgagta cgcccagcgg
     6661 atggccgaga acgaggcgga cctggccaag gcccggcagg aatggcgcga cgccatcgat
     6721 gaggcccgca agaagcgcga ggcaaaggaa gccgagggcg gccccgatgg gctggaaggt
     6781 ccagatgaca tcatcaacaa ggcccgcgac gccctggccg gattgggtga catcggggat
     6841 ctggtgcaac aggaagcggc gaagatcggc gtcaagggca cgttcaatgc cgccgccgtg
     6901 cagggcctgg ccgccggtga tgccgccgat cgcacggcca aggccagtga agagactgcc
     6961 aagaacacca agaagctcgt ccaggccgcg cagaccggcg gcctgacgtt cgcttgaccc
     7021 ccggaatcag gagaggcacg gatgccgatc gtctgcaccg agaacatcga ctcacgccag
     7081 atcaccgaaa cccagtcggc ggagttgctc tacacgatca gcggcacggc cgatgaagcg
     7141 gcggccatcg cctcgctcaa ggcgaccgcg ccgacggtgc tgcacaacat gaagcgccaa
     7201 ccggtgacgg tcgagccggt ccacgtcgac accaccaacc ccgacaagtg catctggacc
     7261 ggcacggtca actacgcgcc cttcgagtac caggagccgc cggagaccgg cgattcgtca
     7321 ttcagcttcg acaccggcgg cggtacgcag cacatcacgc agtctctgtc cacgatcggc
     7381 cggtacgctg cttccggcac cgcccccgac ttcaagggcg cgatcggcgt cacgcacgac
     7441 aacgtcgagg gcgtggacat taccgtaccg gtctacaact tctccgagac gcactacctg
     7501 tccgcagcga cagtcaccac ggcctacaag ggcacgctct tccaactcac cggcaaggtg
     7561 aacagcggtt cgttccgtgg cctggctgcc ggtgaatgcc tgttcctggg cgcgtccggt
     7621 tcgcggcgcg gcactggccc cgatgacgat tgggagatca ccttccgctt cgccggttcg
     7681 cccaaccgca cgggcatcac cgtggggccg atctctggta tcgccaagaa gggttgggag
     7741 tacctgtggg tgcgctacgc cgacgcggaa gacactggca gcaacacgct ggtcaagcaa
     7801 cccatcgcgg cctacgtcga gaaggtctat gaagacggca acttcgcggc attggggatc
     7861 ggatcatgag cgatggcttg aagaaggtcc aatccggcca gccgctgacc atccccgcca
     7921 gcgcctacaa cgccttcatc gacgcggcgc tggactttcg gcagcgcacg gcccacctgg
     7981 gccaaggggc tcaaccctcg ttctcgcagg ccagcatcgt gctggtccgc aacgacagtg
     8041 gctcgaatca gacccgcatg gcggtattgg gcgtcgatac gccgatcatc gatccgtcgg
     8101 ccaacgagga ggagttcaag aaccgcgtgg cgctctcctg cgtcgcgccc gccgccgaca
     8161 cgcacgaggg caagttcgtc atcctggccg aacccatcgc caccggcaag atcggacgcg
     8221 cctacgccgc cggtgtctgc ccggtgaaga tcgacgtgcc cgacgaagac cacgagtggc
     8281 gctacgccga gatcgccgac ggcgtcacgg ccaacctgaa ggtgagcatg caagggtcgg
     8341 cgggcgtcct ctggcgcgcg ggcgggaccg gcgtgcagtg gtcggtggtc cgtctgggca
     8401 agcccacccc aatgcatgtg ttccccgttg acctcacgca gtacgggggc gagcagggcg
     8461 acgaggagaa tccggcaacc tggacgtatg acgtgctgga cgtggtgacc ggcgagacgc
     8521 tggagagcgg cgtcgatccc gtggccgcgc cgcacaagtg gcagcgtccc agcatcggcc
     8581 agatgatcgc cgcgaccttc ggctatgccc attatcaggg tgatggggcg ggcggtctgg
     8641 aactggtgct cggctggatc aacgagatgg tcgaccagga ggcctgcgag acggccagca
     8701 gcaccgagta aggaggccag cgtgggcgca ccgggtaaat cagtcgtggt cgaaggcggc
     8761 aaacgcggcg tgctgctggg cggcaagtcg gcggtctaca acgccgagga gatctgcccg
     8821 gcctgctgca tcgagttcac ccagcagtgg tcattcaccg attcggggtt catcgacggc
     8881 gggcaggacg gcgctttccg cgcctacgac aatcccgccg acgtgcccgc cagcccctgg
     8941 tcgatcctga acaacggcct gggcctgcga ctggactggg aagacgacca gaactgccgc
     9001 agccacaatc cctacacgca gtacgccacg gcgacctgcc agatcaccgt gcccaaggcg
     9061 atgatcatga cggtcaactg gtcgggcatg ggcgagacgc aggatcccaa ctacgaactg
     9121 atgagcctgt atgtgaacgg cgggctggtc ggatcggccc atgctcccgg cggcggtcag
     9181 ggctgcgcgg gtatgggacc ggtcatctcc aatccgccgc cgccccaaca ggtgctgctc
     9241 aatcccgggc cgcacacact gttcatcgac gccacgacaa acgatccgct ctaccacttc
     9301 ggggcgtggt atcggttcga cctgaccttc gagctggccc cctgaggagc atgacatgag
     9361 cgagacgttg acgcccaagc agaagaagcc ctgcacgaac tgcccgccgg tggtgatccc
     9421 gcgccgggcc tatacgccca cgcggccgag ttgtgtcgag tgcgtcgaga aacacctggg
     9481 cgcggccctg gtgctcctga ccgaaacccg tgagggctac gcctaccgcc ttcgggccat
     9541 cgggcatctg ttcgaggccg aggacgagag ccaggagtgg cccgagttgc acgccgccat
     9601 ccgcgaggct cgcaaggcct accaagcgga cggcacgctg ccggaatggg aaaccttggc
     9661 agagacggtg gttcgcacgg cggcggtgaa gacgtgatgc agtgccgggg gcgtagaacg
     9721 cgaaaacagg cgattttgag actttcgctt gtaaacccct gtgaatgcgg gggccagcgt
     9781 tgtctcacgg tctcagccgc gtgagacggc tgagacttcg ggagggggca gaggcgcaga
     9841 atgcggaaat acccgattct ggcactttgg ctcgtaaacc cttgtcagca caggggccga
     9901 tctggtgcca cgccttcgcc aaccggcacg gtctcaaccc cctgatcgaa cgtcaggcac
     9961 ttccagagcc gtcgttgctt gcgccagtcc ggttcggcgg cgatggatcg aaccatgatc
    10021 tcgcggatgg ggtcgcgccc ccggaaggtg cgcggcagga acaggatgtc ttcctggatg
    10081 tccggcgcga gttggagcag attcatgatc tgcgtcacgc gggcgcgggt cacgtggccc
    10141 agacgggcca ggtcggcctg atcggcaatc tcgcccgcct tgatgagccg gtcgaaccgg
    10201 atcgccaggg ccatcagacg cgagatgcgc gggaccgtgc cctctggcgt cgcgtccagt
    10261 tccggcggcg ggccttcctg gatgaccttg cgggtgcgct gcccccgcgt gaaatgcacg
    10321 tcgcattcaa tcctcaagcc tttcatgcgg tcacctcctg tcggttcagt tcatcggcca
    10381 aggtcttgat gcccgtgggg tggaaggtga tggcgacctt cccgttaggg ccgtcgt
//
LOCUS       DAOTPW010000205        10428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_5985_length_10428_cov_5.312446, whole genome shotgun sequence.
ACCESSION   DAOTPW010000205 DAOTPW010000000
VERSION     DAOTPW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5985_length_10428_cov_5.312446"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1228
                     /locus_tag="P5316_16450"
     CDS             230..1228
                     /locus_tag="P5316_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR86603.1"
                     /translation="MAACLPQKPNIRTRRRGTDQHSAGSPASTAGFTLIEVLVVVAII
                     ALLIAILLPSLSLAREQARRVVCLVNMSNLPKAALSFSMEHKGYLQLIGREDEWTVID
                     SGLTKYAYQAGCFGQTTAQLKAWPVAYAPQLSMPSLKRNEQYFDTALKSEPAYYFEKF
                     GRRDIFICPSDKVLVNNVWSPFDMFGVISYAANEDVMGVTNPHDGEGKPWNNGVREGG
                     RRLEGRMDKVIRPSEVVLFCDGGNEDVSGEPALLITNGNINGPYIENYEREWGRLPHF
                     RHSKKGGLACAFADGSGQYLKPLEWTTISGKMYVKRYGPRARVSPYEVGQLKTVQP"
     gene            complement(1438..2025)
                     /locus_tag="P5316_16455"
     CDS             complement(1438..2025)
                     /locus_tag="P5316_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86604.1"
                     /translation="MRTGLLHTCVCILTFAGAAQAALLSYEFQPTPVDLYDLDHAYAY
                     RWGLDWSPPDDTVVVSAKLEIENLKNWTVEPNILYIHLLDIVPTGVTRQYDNRPGDWF
                     EGQGVLLTTFTDTRTSNSQPAVDWSYDFSAQDLVVLNSYVADGTFGLGFDPDCHYYND
                     GVKLTLLVDHTPEPAMLALLGGPLPLVLKRRRRHG"
     gene            2520..4025
                     /locus_tag="P5316_16460"
     CDS             2520..4025
                     /locus_tag="P5316_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRR86605.1"
                     /translation="MKRDLPRSIGFWGGSGIMVGIMIGSGIFRTPASIAAEMGDARLI
                     LLLWAVGGTLSLAGALTFAELGTMFPRSGGIYAYIYEGLGGTVAFVFGWTYMLLGKPL
                     AASAITMVCAEHFNKLLGLNWNVPAMTCLVLIVLTAINTVRVQLGAGLAIFLTSLKAL
                     ALAAIIVLGLLLTGGSSENLAAVDSPKPFLAALAPVLAAVLWTYDGWADVTSVAGEVR
                     EPGRMLPRILLVGTAVTAVLFVAVNATYIYVLPLSEMRGVPWVVPLVMERLCGPVGGT
                     LATILVMISTLGASHGSIITGARVTFAQARDGLLFRVLGRIHPKYQTPYVSLWCQAIL
                     ACVAVCFLKQFERLTGGFVFTMWIFYAGAAVSLIVLRIRRPDLSRPCRCWGYPILPVL
                     FIAASALMTVLAIKASPKETLFWLAVLVVGTPAYYLWRWLVPIEQCESVGMCPGCGCN
                     LYGLRDQRCPECGRPFTFEDIRSTPQSLGFMVAEPQVEESSEPEKPTGERH"
     gene            complement(4077..5159)
                     /locus_tag="P5316_16465"
     CDS             complement(4077..5159)
                     /locus_tag="P5316_16465"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRR86606.1"
                     /translation="MSMHQWTGLTRRRFLESAACGLALDRMALAATATGSGDEPFFLT
                     RGVVITEEDLTLADWPERAAAAGLTTIGLHAPKSPRLLARYIQSDGGQKFLETCRRLG
                     LGVEYELHAMQDLLPRELFDKAPELFRMDDKGSRVREWNLCVHSERAMQTVAENAVDL
                     GKTLRPTTGRYFYWGDDGCPWCRCRQCAGLSDSDQSLLVANRILDAIRKVDAKAQVAY
                     LAYDNTLPPPKQIKPKSGVFLEYAPIHRRYDVPFERADDLKTRQQFEMLDANLDVFGR
                     ANAQALEYWLDVSLFSKWKKPAVKVPFDPKVLTADLDTYGRRGIRHVTTFAVYIDADY
                     VSRHGEPPLKDYGEQLRRWRRQPSHG"
     gene            complement(5251..6342)
                     /locus_tag="P5316_16470"
     CDS             complement(5251..6342)
                     /locus_tag="P5316_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86607.1"
                     /translation="MCSRSTPLCVSGVIGFGVLLGLALAQETKPARRAVSEQTTTEQK
                     PAGERPERPIEISGPFAHDNLAVYFVHGEDRLKGRDLLTLAEALEQKKARILETSDVN
                     ELTIENLSKDQEVFVQAGDIIKGGKQDRVCGDDLIVGAKSGKVKLRVFCVEQGRWEQR
                     GNETAVLFSSSYNVVPSNALKATVRRKGDQQQVWAGVAETQKKLSMNVGTPVASQASD
                     TSLQLSLENKELRKQTAEYTDKLSEALAGREDVIGVVIAINGGLESADTYASPKLFKK
                     LWPKLLEAGATEAIARRDEKKKDVSPPSIKVVEEFLTASTDKAKTTQVSKRIRTVERE
                     SKQMIMFETLDAQQGDAAVHESYMIKSQE"
     gene            6824..7936
                     /locus_tag="P5316_16475"
     CDS             6824..7936
                     /locus_tag="P5316_16475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRR86608.1"
                     /translation="MELDQIHHLDCIEGLGKLPEGSVDLAFADPPFNIGYDYDVYDDR
                     RASDEYIAWSRKWIEGVIGVLKPNGTFWLAIGDEYAADLKVLCTRELGLHCRSWVIWF
                     YTFGVHCKQKFTRSHAHLFHFVKDPRNFTFNSLAVRVPSARELVYGDKRAAPDGRMPD
                     DTWMISPILPPEVPTKDGFVLRPQDIPERFPPHSDTWYFSRVAGTFGERRGWHGCQMP
                     EQLLGRIIRACSHEGELVLDPFGGSGTTLAVAKKLNRRFIGFEISDKYVKNIKERLSS
                     IQPGDPLVGPENPVLSAPPTKAGRKLNRSKDQATKPVPKSRIRTAMTRKDSETGCVQA
                     PLGFLPTPIAKSNSSGNSASKRPASSRTARAAKCAG"
     gene            8068..9003
                     /locus_tag="P5316_16480"
     CDS             8068..9003
                     /locus_tag="P5316_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRR86609.1"
                     /translation="MNRVRQSVVVLSVSLAAGCAAAGQRGVTAPGASVERLSREFEFT
                     EGATCDAEGNVYFTDQPNDRIMRWSVDGRLSVFMKPSNRSNGMMFDTKGNLYSCADEK
                     NELWMITPDKKVTVLVKEYKGKLLNAPNDVWLRPDGGLYFTDPWYKRDWWKRGPQEQE
                     VEGVYYLAPHREEDKPGAPRQLTRVIDDLKTPNGITGTPDGKTLFVSDLGAQRTYVYD
                     IRPDGSLTNKRLFCQMGSDGMTIDSEGNVYLTGKGVTVFDKAGNQIDHIDVPEEWSAN
                     VCFGGKHRRTLFITAGKGLYAVKMRVKGVNDKGGK"
     gene            complement(9319..>10428)
                     /locus_tag="P5316_16485"
     CDS             complement(9319..>10428)
                     /locus_tag="P5316_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86610.1"
                     /translation="AIRDGAYGRDAFLRGDPFIGWDGTNVNAVHSISSYAVLVGRSMK
                     TPSTAFFESSSSANPFTDVPGSSGFRADGHGISSSFGKSATSDFDVQWICGQAQNYNG
                     PGTTMQAFRWKRGDASMTFLGSLAPPGGGDTGNNSSCAYAIADNGITAGRSFFGAAGG
                     NPSYEVATVWGIDGLPQSLEAMLADAGVDTSEWTHLVRVYAISDDGKTLAGMGTWAAD
                     GSTRGFVAHLDDFIVKGACCVTTGLGTGTCSHVTRAECDALGGRYLGDGVQCGANNFN
                     CGGFCPIPFADADVDGDVDQADFAAFQACFTGPLGGSPGLADACACLDRGLGASDGDI
                     DADDLAAFEACATGPGIPWVAEQLPLCVPGDGTLP"
BASE COUNT         2001 a         3369 c         3014 g         2044 t
ORIGIN      
        1 tcaaatctca aatcccccaa accccgctcc ctgcgtccag aaagcttctc attcccaccc
       61 tttcaacgtg caggcttcgg atttcatcgg tcattccgat tacggctcct cgtcggttgc
      121 gtctggaagg caagggcggt acaattcgtc ttaccgcggc gggcgaaagg atggtccctc
      181 gcgtccggta tcgatttcgg gctgcaatgt tgctcggtga ggtggaacaa tggcagcgtg
      241 cttgccgcaa aaaccgaaca tacgcacccg acggcggggt acggatcagc attccgccgg
      301 ttcacccgcg tcgaccgccg gcttcacgtt gatcgaagtc ctggttgtcg tcgcgatcat
      361 cgccttgctc attgccatcc ttctgccttc tctgtccctc gcgcgggaac aagcccggcg
      421 agtcgtatgt ctggtcaaca tgtcgaactt gccgaaggct gctctctcct tctcgatgga
      481 acataagggg tacctgcaac tgatcggccg cgaggatgaa tggacggtca tcgacagcgg
      541 cttaacaaag tatgcctacc aggcaggctg tttcgggcaa acaaccgccc aactgaaggc
      601 ctggcccgtt gcttacgcgc ctcaactcag catgccgtcg ctcaagcgaa atgagcaata
      661 cttcgacacc gccctcaaat ccgagccggc ttactacttt gagaagttcg gccgacgcga
      721 catcttcatc tgcccctcgg ataaggtgct ggtcaacaac gtctggtcgc cgtttgacat
      781 gttcggcgtc atctcgtacg ccgcaaacga ggacgtcatg ggagtgacca acccccatga
      841 cggcgagggg aaaccgtgga acaacggcgt tcgcgaaggc ggacggcgac tggagggccg
      901 catggacaaa gtcatacgcc caagtgaggt agtgcttttc tgcgacggcg gcaacgaaga
      961 tgtcagcggc gaaccggcac tcctcatcac caatggcaac atcaatgggc catatatcga
     1021 gaactatgag agagaatggg gccgcctgcc tcacttccgc cacagcaaga aaggcgggtt
     1081 ggcctgtgcc tttgcggatg gatcggggca gtatctcaag cccctggaat ggaccaccat
     1141 cagcggcaaa atgtacgtca aacgatacgg ccccagagct cgggtatccc cttacgaggt
     1201 gggacagctc aagaccgtgc aaccctgaaa acggacgctg gtcccttgag aacccccggt
     1261 tccggcgggg tgctgcgcga cgatcgaggt ccccgtcagc caattctcct ggtatgccgg
     1321 tcgaacatcg ctcccagaat gcgtgcgaga accgacggag tccgtacgaa agggtcttgg
     1381 tcgccgtggc ggccaagacc ctcccggatt ctttgcttct tgcgttgttc ctgcccgtca
     1441 tccgtgccga cgccggcgtt tgagcacgag aggcaacggc ccccccagca gagccagcat
     1501 cgccggttcg ggcgtatggt cgacgaggag cgtgagcttc acgccgtcat tgtagtagtg
     1561 gcagtccgga tcgaaaccca ggccgaacgt gccgtcggca acatagctgt tcagaacgac
     1621 cagatcttgc gccgagaagt cgtatgacca gtccaccgca ggctggctgt ttgatgtccg
     1681 ggtgtcggtg aaagtcgtca ggaggacgcc ttgaccctca aaccagtcgc ccggccgatt
     1741 gtcgtactgc cgggtcacgc ccgtcgggac aatatccagc agatggatgt agaggatatt
     1801 gggttccacg gtccagttct tcaggttctc gatctcgagc ttggccgaca cgaccacggt
     1861 gtcgtccgga ggggaccagt cgagacccca cctgtacgca taagcatggt ccaggtcgta
     1921 aaggtcgacc ggcgtcggct gaaactcata cgagagcagg gcggcctgag cggcacccgc
     1981 aaaggtcagg atgcaaacac acgtgtggag tagtccagtt ctcatttctt ctctcccctg
     2041 tgcaaacggc gaaaatcgca gaatgctatg ctcgcacctc gcagcaagtc aaagcccgtt
     2101 cacaagattt ctggttatca gacgacgacc atacggccag acagcgcttt atcggccatc
     2161 ccgaacaatt gtgccccgcc tcacacggcg cactgcgcgt catgaagaat cccggacaaa
     2221 cagtggcggg tgccttgccg ccatccggga aacgaacttc cgcaaaccgg caggatgctc
     2281 ggcaaccatt tgattgccca gggaccaccc catagaatcg cggccatgat cgcgagtgtt
     2341 caagcgacgg catcggcatg tccgcagccg gttgtatacg tgcccggttg gacgctcgca
     2401 cctcacgacg ctacctcaaa tgcgatgttg tcagtcgtta catccgctcg acccagtgtc
     2461 tcccggcggt tcctctcctc gccatacgac gggcgaggat gccatctgag agggctgcga
     2521 tgaaacggga tctgccgcga agcatcggct tctggggcgg ctccgggatc atggtcggga
     2581 tcatgatcgg cagcgggatc ttccgcaccc cggcctcgat cgccgctgag atgggcgacg
     2641 cgcggctgat cctgctgctg tgggcggtcg gcgggacgct gtccctggcc ggggccctta
     2701 ccttcgctga actcggcacc atgttcccgc gatcgggagg catctatgcg tacatttacg
     2761 agggcctcgg cgggaccgtg gcctttgttt tcggctggac ctacatgctg ctcggcaagc
     2821 cgctggcggc gtcggccatc accatggtct gcgccgagca cttcaataag cttctcggcc
     2881 tgaactggaa cgtgcctgcc atgacttgcc tggtgctcat cgtgctcacc gccatcaaca
     2941 cggtgcgcgt tcaactcggc gccgggttgg ccatcttcct caccagcctg aaagccctgg
     3001 ctcttgcggc gatcatcgtg ctcggtctgc ttctgaccgg aggttccagc gagaatcttg
     3061 cggccgtcga ttcgcccaag ccgtttctgg ccgccctcgc cccggtgctg gccgcagtgc
     3121 tgtggaccta cgatggatgg gccgacgtca cctcggtcgc cggcgaggtc cgcgagcccg
     3181 gccgcatgct gcctcgcatt ctccttgttg gaaccgccgt caccgcggtt ctttttgtgg
     3241 cggtcaatgc cacgtacatc tatgtgctgc cgctgtccga gatgcgcggt gtgccttggg
     3301 tggtcccact ggttatggag cgcctgtgcg gccccgtcgg cggaacgctc gccacaattc
     3361 tggtgatgat ctcgactctt ggggcatcgc acggctcgat catcaccggt gcccgggtta
     3421 ctttcgccca ggcccgtgac ggcttgctgt ttcgcgtcct cggacgcatt catccaaaat
     3481 accagacgcc ttatgtctct ctctggtgcc aggccatcct tgcctgcgtg gccgtctgtt
     3541 tccttaagca gtttgagcgt ctgaccggcg gctttgtctt taccatgtgg atcttctacg
     3601 ccggggcggc cgtatccttg atcgtattgc gcatccgacg cccggacctc tcccgcccgt
     3661 gccgctgctg gggatatcca attctgccgg tgctcttcat tgcggcctcc gcattgatga
     3721 ccgtgctggc catcaaggcc tctcccaagg agactctgtt ctggctcgct gttcttgtgg
     3781 ttggcacgcc ggcctactac ctgtggcggt ggttggtgcc gatcgagcaa tgcgagtccg
     3841 tcgggatgtg tccgggctgc gggtgcaacc tctatggcct gcgagaccag cgatgtcctg
     3901 agtgtggccg cccattcaca tttgaggaca tccgctcgac gcctcagtca ctcgggttca
     3961 tggttgccga gccccaagtc gaggaatcgt cggaacccga gaaaccgacc ggcgaacgtc
     4021 actagccgaa tccatgcttg cctgtctcac cgaattggcc gccttgcgat gtcgttctaa
     4081 ccatggcttg gctgccggcg ccatcgccgg agttgctcgc cgtagtcttt caacggcggc
     4141 tcgccgtgac gcgatacgta gtccgcgtcg atgtacacgg cgaaggtcgt gacgtgacgg
     4201 atgccgcgcc ggccgtaggt atcgaggtcg gccgtcagga ccttcggatc gaacgggacc
     4261 ttcacggccg gcttcttcca ctttgagaac agcgagacat cgagccagta ctccaacgcc
     4321 tgtgcgtttg cccgcccgaa gacgtccagg ttagcgtcga gcatttcgaa ctgttgccgg
     4381 gtcttcaagt cgtcagcccg ctcgaagggc acgtcatatc gccgatggat cggtgcgtac
     4441 tccaggaaaa cgcctgactt cggcttgatc tgcttcggcg gcggcagcgt gttatcgtag
     4501 gccaggtacg ctacctgcgc cttcgcatcg accttgcgga tggcgtcgag gatccggttc
     4561 gcaacgagca gcgactggtc gctgtcgctc agcccggcac actgccggca gcggcaccag
     4621 gggcacccat catcccccca gtagaagtac cggccggtgg tcgggcggag cgtcttgccc
     4681 agatcgacgg cgttctcggc caccgtctgc atcgcccgct ccgagtggac gcacaagttc
     4741 cattcacgaa cccgcgaccc cttgtcgtcc atccggaaaa gctcgggggc cttgtcgaaa
     4801 agctctcgcg gcagcaggtc ctgcatggca tgcaactcat actcgacacc caggccaagc
     4861 cgccggcagg tttcgagaaa cttctgtccc ccgtccgatt gaatgtacct ggcgagcagc
     4921 cttggcgatt tcggtgcgtg cagcccaatg gtggtcaggc cggcggccgc ggcccgctcg
     4981 ggccagtcgg ccagcgtcag gtcctcttcc gtgatcacca caccgcgcgt gaggaagaaa
     5041 ggttcatccc cacttcctgt ggcggtggcc gcgagtgcca tcctgtccag tgccagcccg
     5101 caggccgccg attcaagaaa ccgccgccgt gtgagtcctg tccattggtg catgctcatg
     5161 gcacaagcct accgcctcat caccatcaac gccacctccc cgtcggcgtc acctgctgca
     5221 gatgatcgag catcgcgcct ggttcgacca tcactcttgc gactttatca tatagctttc
     5281 atgcaccgcc gcatcgcctt gctgagcatc cagtgtctca aacatgatca tctgctttga
     5341 ctcccgttcg actgtcctga tgcgcttcga cacctgagtc gtctttgctt tgtccgtcga
     5401 ggcggtgaga aactcctcta cgaccttgat cgacggcggt gaaacgtctt tcttcttctc
     5461 gtcccgccgg gcgatcgcct cggtggcccc ggcctccaac agcttgggcc atagcttctt
     5521 gaacagcttc ggcgaggcgt atgtgtccgc actctccaga ccgccgttaa tggcaatcac
     5581 cacaccgatc acgtcttccc tgcccgcgag cgcctcggac agcttgtccg tgtactccgc
     5641 cgtctgcttg cgaagctcct tgttctccag cgacagttgc aggctggtat ccgacgcctg
     5701 gctcgccacc ggcgtcccca cattcatgga aagcttcttc tgcgtctctg ccacgccggc
     5761 ccaaacctgc tgttggtcgc ccttccgtcg cacggtcgcc ttcagggcgt tcgagggtac
     5821 cacgttgtac gaactcgaga agagcacggc cgtttcattt ccccgctgtt cccaccgtcc
     5881 ctgctccacg cagaagaccc tgagcttgac cttccccgat ttcgccccca cgatcagatc
     5941 gtccccgcac acacggtcct gcttgccgcc cttgatgatg tcaccggcct gcacgaagac
     6001 ctcctggtcc ttggaaaggt tctcgatagt caattcgttc acgtcggaag tctcgaggat
     6061 cctggccttc ttctgttcaa gcgcctccgc cagcgtcagc agatctcgcc ccttgagccg
     6121 gtcctctcca tgcacgaagt acaccgccaa gttgtcgtgg gcgaacggcc cactgatctc
     6181 gatcggccgc tccggccttt cgcctgccgg cttctgctcc gtcgttgtct gttccgagac
     6241 cgcccgcctt gcgggtttgg tttcttgggc gagggccaac ccgaggagca ctccgaaacc
     6301 gatcaccccg gacacacata aaggtgttga gcgcgaacac atggcgaaat ctcctgaatc
     6361 ggcaaccctt tgactcttgg acccgacttg tggaacaacc gcgagaaccg gcccacccgc
     6421 aatgaatgac tggcaaggtc tctgtgcaat tatacgcatg gaccgagaac cggttccgca
     6481 gaaccggaca accgacaaaa acaacccatc ctcagaatgg agaacccatc acctatcttc
     6541 tgcgccccca tccgtcatcc ctcacgaccc tcctgctata ccctgattgt gccgaccaag
     6601 gcggcggtcg tgtgcctgcc cgaccggtgg cgaatagggc ggggaaacac gttttcgagc
     6661 attccgagac ttgtgtcgag cccggaccgc tggattccgg ttacttgaca actacctaac
     6721 cccttgctaa cctacgcacc ccgatgtgag cgccgatggg aacgtcggac tcaagatatt
     6781 gcggtccccg tcgatcgtgc taccataagg cgcggaggac gccatggagc tcgaccaaat
     6841 ccatcatctg gactgcatcg aaggccttgg caagctgccc gaggggtctg ttgacctggc
     6901 ctttgccgat cccccgttca acatcggcta cgactatgac gtctacgacg accgccgcgc
     6961 gagcgacgag tacatcgcct ggtcgcgcaa gtggatcgag ggcgtcatcg gggtgctgaa
     7021 gccgaacggg accttctggc tggcgatcgg cgatgagtat gccgccgacc tcaaggtgct
     7081 gtgcacgcgc gagctggggc tgcattgccg cagttgggtc atctggttct acaccttcgg
     7141 cgtgcactgc aagcagaagt tcacccgcag ccacgcccat ctgttccact tcgtcaagga
     7201 cccccgcaat ttcaccttca actctctggc tgtccgcgtc ccctcggctc gtgagctggt
     7261 ctacggcgac aaacgagccg cacccgacgg ccggatgccg gacgatacct ggatgatcag
     7321 cccgatcctg ccgcccgaag tcccgaccaa ggacggcttc gtcctgcgcc cgcaagacat
     7381 ccccgagcgc ttcccgccgc acagcgacac gtggtacttc tcgagggtgg ccggcacatt
     7441 cggcgaacgc cgtggctggc acgggtgcca gatgcccgag caactcctcg gacggatcat
     7501 tcgggcttgc tcgcatgaag gcgagctggt gctcgatcct ttcggcggaa gcggcacaac
     7561 cctagccgtc gccaagaagc tgaatcggcg gttcattggc ttcgagatct ccgacaaata
     7621 cgtcaagaac atcaaggaac ggctgtcctc gatccagccg ggtgatccgc tcgtcgggcc
     7681 ggagaatcct gtcttgagcg ctccgcccac caaggcaggc cgcaagctca accggtcaaa
     7741 agatcaagcg accaaaccgg taccgaaaag tcgcatcagg accgcgatga cacggaagga
     7801 ttccgagacc ggttgcgtgc aggcgccgct gggattcctc cccacgccca ttgccaagag
     7861 caattcgtcg ggcaactccg cctcgaagag gcctgcttcg tcgcggactg ctcgtgcggc
     7921 gaagtgtgcc ggttgagaga aaccggccga agaaaaatcg tgacaaacgc gatacaagcc
     7981 tggcccacat gcaagaatgc gtcccgacat gacggccggg ggccggatcg ttgcgcgtct
     8041 tccgtgtgca ctgcgaggga acagaacgtg aaccgagtca ggcagtcagt agttgtcctg
     8101 tcagtttcgc ttgcggctgg atgtgcggcg gctgggcagc ggggcgtgac cgcgccgggt
     8161 gcgagcgtcg aaagactatc gagggagttt gagtttaccg aaggggcaac ctgtgacgcc
     8221 gaaggcaacg tctatttcac cgaccagccc aatgaccgca tcatgagatg gagtgtcgac
     8281 ggcaggcttt ctgtgttcat gaagccgagc aatcgatcca acggcatgat gttcgacaca
     8341 aagggaaacc tctattcctg cgcggacgag aagaacgaac tctggatgat cacgccggac
     8401 aagaaagtga ccgtgctggt gaaggagtac aagggcaagc tgctcaacgc gcccaacgac
     8461 gtgtggctgc ggcccgatgg cggtctctat ttcaccgacc cgtggtacaa acgagactgg
     8521 tggaagcgcg ggccgcagga gcaggaagtg gaaggcgtct actacctcgc accgcatcgc
     8581 gaggaggaca agcccggcgc gccgcggcaa ctcacccgcg tcattgacga tctcaagacg
     8641 cccaatggca tcaccggcac cccggacggc aaaacgctct ttgtctccga cctgggagcc
     8701 cagcgtacgt acgtttatga catccgcccg gacggatcac tgacgaacaa acgattgttc
     8761 tgccaaatgg gctcggacgg catgaccatc gacagcgaag gcaacgttta cctgaccggc
     8821 aagggcgtga ccgttttcga caaggccggc aaccaaatcg accacatcga cgtgccggaa
     8881 gaatggtcgg ccaacgtctg cttcggcggg aagcaccgca ggacgctctt cattacggcc
     8941 ggcaagggac tctacgcggt gaaaatgcgc gtcaagggcg tgaacgacaa gggcgggaag
     9001 tagatcaacc tcccattacc acaaagacct catcactcat gccctggcgt gcggcacgca
     9061 acacgaaaga cgtcgtctta tgggatctct tccgatacgg ccggcaaaca cgccatcccc
     9121 ggcactcggt tctcgatcac ccggctcacc ggacgcccaa gcaaggacga cattctgctg
     9181 ccgaacgggg gaactgctga tctcgccggc cgcgctcatt accgcccttc ggctgccatg
     9241 tctctgcgac caggacgact cctgataacg tagcttgtca ccgttcgatg aacgacggtg
     9301 taccccccct gggtgttctc aaggcaatgt gccgtcacct ggaacacaca acgggagttg
     9361 ctcagcgacc cacggaatcc cgggacccgt ggcacatgcc tcgaatgccg ccagatcgtc
     9421 ggcatcgatg tcaccgtcgc ttgcgcccag cccgcgatcc agacacgcac acgcatccgc
     9481 gaggccgggc gagccgccga gcggcccagt aaaacaggcc tgaaacgcag cgaaatcggc
     9541 ctggtcaacg tcaccgtcca cgtcggcatc agcaaagggt atggggcaga aaccaccgca
     9601 gttgaagttg tttgcgccgc actggacccc atcgcccagg tatctgcctc ccaaggcgtc
     9661 gcactccgca cgcgtgacat ggctgcacgt cccggtaccc agaccggtag tcacgcagca
     9721 ggcacctttg acgatgaaat catcgaggtg ggcaacgaag ccgcgggtgc tgccatcggc
     9781 tgcccaggtg cccatgccgg ccagggtctt gccgtcgtcc gaaatcgcgt acacccgcac
     9841 cagatgagtc cactcgctgg tatcgacgcc ggcatcggcc agcatcgctt ccagcgactg
     9901 tggcaacccg tcaatgcccc agaccgtggc gacttcatag gaagggttcc cgccggcggc
     9961 accgaagaag ctcctgccgg cagtgatgcc gttgtccgcg atcgcgtaag cgcaactgct
    10021 gttattgccc gtgtcgcctc cccctggcgg tgccagcgag ccgaggaagg tcatcgacgc
    10081 gtcgccgcgc ttccaacgga aggcctgcat ggtggtcccg gggccgttgt agttctgagc
    10141 ctgcccgcag atccactgca catcgaaatc gctggtagcg gatttcccaa agctggagga
    10201 aatcccgtgg ccgtcggcac ggaaaccgct gcttcccgga acgtcggtaa acggatttgc
    10261 cgaactcgac gactcaaaga acgcggtact cggggttttc atgctcctgc ccacgagcac
    10321 ggcataggaa ctgatggaat gtactgcgtt gacgttggta ccgtcccaac cgatgaaagg
    10381 atcgccgcgc aagaaggcgt cccgtccgta agcaccatct cttatagc
//
LOCUS       DAOTPW010000206        10316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6054_length_10316_cov_4.291102, whole genome shotgun sequence.
ACCESSION   DAOTPW010000206 DAOTPW010000000
VERSION     DAOTPW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6054_length_10316_cov_4.291102"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="P5316_16490"
     CDS             complement(<1..282)
                     /locus_tag="P5316_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86611.1"
                     /translation="MAKSAANPTRPTFGPDEIAKAKECFTRAQELVSKKNYDYAIELY
                     ILGLGHWPEAVEEGHKPCRAAALFRGPKKVGFVDQMKYKTSGKDYKTCML"
     gene            617..1150
                     /gene="def"
                     /locus_tag="P5316_16495"
     CDS             617..1150
                     /gene="def"
                     /locus_tag="P5316_16495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRR86612.1"
                     /translation="MLVETLPSMIVRYPDPRLRRKCEPISVFDDSLAALVERMFQIML
                     RDRGVGLAAPQVGLSCSLFVANPTGNPQDNAVYINPKLSELVGSVEGEEGCLSFPDVR
                     ANIRRARRCRIQAQDLAGNPIDQIGEDLVARIWQHEMDHLDGRLIVDHMNFADRLANK
                     KSLSDLEASYRKRAKRR"
     gene            complement(1489..3201)
                     /locus_tag="P5316_16500"
     CDS             complement(1489..3201)
                     /locus_tag="P5316_16500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRR86613.1"
                     /translation="MPQCRHLLPIRLCLCTALAAAASGQAADPKASLTTMVPMTDGTL
                     LATDVYLPATGGPQWPVRVVRTPYGRTRFNREYGAKADRGYVMVIQDMRGRFDSEGKD
                     LAFIDCGWGDNRDGLDTIKWILDQPWCNGKIGTEGASAMGITQCMLAPTDPPGLVAQY
                     IMVAAPSLYHHASYVSGALRAALVDGWLTGAGFHPDNLWLTCLHPFYDGHWRKLDSVA
                     ESHRIKVPAVHSGGWYDVFLQGTIDGFVSRHNDGGPGAKGKQKLIIGPWGHGGPKRDS
                     DLPLAAKASPIGELTFPANSLKTPFSCGADEWFDFHLKGIDTGVEKVPAVQYYTMGAV
                     GEEDAPGNQWHTADNWPVPAKPTPFYLYSDNSLSRKAPETTSEQVAFIHDPLNPVPTR
                     GGCLLLSWNEYGTKRKVAPGVYDQRDIEQRPDVVTFTSEPLAEPLEVTGRLTARLFVA
                     SDRIDTDFAVKLTDVYPDGRSMNIADGLARCRYRKGFDRLTLLQPGQPTEIEVDLWST
                     SMVFNKGHRIRVAVTGSNYPRFDVNPNTGWPAWPMGTMLKAHNQLFCGREHPSRIILP
                     VTGK"
     gene            3485..4531
                     /gene="cdaA"
                     /locus_tag="P5316_16505"
     CDS             3485..4531
                     /gene="cdaA"
                     /locus_tag="P5316_16505"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRR86614.1"
                     /translation="MSYTLGAAGVKLVEALSGPSEPMIQAMLNYLRSIFTSEAYPPFQ
                     VLLEMALIGTVVYSVLRFLHGTRGARLLQGLIILLVVGFLLVNVMAEFLGLDRLTVLY
                     QPFLLSVLLATLVVFQPELRRGLMRLGETRWRRISQSEIDRVAVPVAAACTQLSKNKI
                     GALIAIERDVGLSAIAEEGVRLDARLSAELLNTIFWPGSALHDLGVVIQNGRIAAAAC
                     EFPLVDAEGVDHSMGSRHRAAIGLSLESDAIVVVVSEETGSISIAERGRLYPHIRPAD
                     LYDTICRHLSHTRVPLVPREEVVPEPEPEPVPSPAVSEGKVGDEVPVGETLDQEVAAD
                     PAKSPHHEPAEAPA"
     gene            4528..5673
                     /locus_tag="P5316_16510"
     CDS             4528..5673
                     /locus_tag="P5316_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86615.1"
                     /translation="MKTQLRLIASTIVLTVLIWIYANQASHSTTTLLVPVKLALAETG
                     GSWALRVADAPADQPNTRQVKLTVRGPKQAIRMLESETGQGRFALTVKLAEQLTPGKT
                     NSRNLYADLSGSPDLLQRGLNLQSVMPEVIEFEVDRYIRVPVEFKPEAGAFKSALVGQ
                     PTLAEKVTARVLESKVGKDFAVYPLVLPLEDAIREAFDKRRTEEAAGPLSLHFPVQLQ
                     SLRLAVDADFEPPVVHVTVELKDQSSIERKTVRPLSVMVRTQDFFEGYEIEWEDKTGA
                     QFTQSINCRVPVSKLDAFRQIESTGILAYVIIDSSDLPPEPTTAGPTATTVPAPTTSA
                     TYRPKQVHFVFPPGFEDIKPVGPPPTVNLRVNRKTTDATGLPTPAAS"
     gene            5846..7111
                     /locus_tag="P5316_16515"
     CDS             5846..7111
                     /locus_tag="P5316_16515"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRR86616.1"
                     /translation="MSNEALEQYALRLARDARAAGRKLALTSGKQRNEALRAMAAALR
                     EQTDALVAENAKDLAAAEANGLTSAMIDRLKLDAGRVAKMAQAVLEIADQPDPVGETI
                     EGRMRPDGFRVEKRRVPLGVVAIIYEARPNVTSDAAALCIKSANACILRGGKESFHSN
                     VAIGSAISSALAKVDLPAEAVQIVNTTDRNLVPILLKQNESIDLVIPRGGESLIRAVV
                     EQSRIPVIKHYTGNCHVYVDSGCPAEMAESVVLNAKIQRPGVCNAAETILFHRDTADT
                     LLPVIGRKLMDAKVEVRGCAETCKRLAGCKPATEQDWYTEYLDLVVAVKVVDSLQDAI
                     DHINQYGSHHTDAILTPDIRSAEAFVAGVDSGNVMVNCSTRLSDGGVYGLGAEIGIST
                     DKLHARGPMGAADLTTYKWVVTGNGHIRH"
     gene            7128..8333
                     /locus_tag="P5316_16520"
     CDS             7128..8333
                     /locus_tag="P5316_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86617.1"
                     /translation="MKRKVHSALSVFLAIASTSCAKAAVGPAAVLREAPLIRMPGVPK
                     PDAPQGGAVDCNSPCHWDGDTMYIFSSEGRPFRSSGPDLMHLNPESERSTYDNEKGYN
                     GGRWIEATHKAEDGKLYGWYHREPGGVCPGNPLTAPEIGAVVSTDNGMNWRDLGIILK
                     APDDSIFCDTPNKYFAGGNGDFSVILDQQEEYLYFLVSTYHKDPKEQGVAIARMRYAD
                     RDGPVGKVFKWHRGQWKEPGLGGRVTPIFGPTIDWHKPRANVFWGPSIHWNTHLERYV
                     MLLNLARDKDWTQEGIYVSINRDIVNPEGWSKPEKILDAGRLEASKWYPQVIGTNAAG
                     RETDKLAGRVARLFVAGRSRWEIIFLRPGEARPAGVMENPDKAVRTPSPAKPTAKKNT
                     VTKTAPKKK"
     gene            8459..9673
                     /locus_tag="P5316_16525"
     CDS             8459..9673
                     /locus_tag="P5316_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86618.1"
                     /translation="MAQAAAVASTRPFDDAPAGDSAAIQKLLDAAAPKGGTVDLGVGR
                     FRLDRPIIVPEGVCLQGTWQGPHHAQLAKGTVFLVYADVGREDGPPLITLSPSSTVKG
                     ITFFYPEQRIPGTKAYPWTIQGRGMHNSVIDCTFVNAYQAMDFATHANELHYVRNCFG
                     CPLKVGIRVDRCTDIGRIENVHFNPHYWARAEAPGVPQWPDLSRYLWENLIAFDFART
                     DWEYVLNTFCYGAKIGYLFRQTRHGTVNGNFLGIGADWCERAVVVEASQPPGLLITNG
                     EFVGGEGSVVMMDVTASHAGVVQLSNCAFWGPCPTAVRVDGKGSVSLSQCNFLNHGKP
                     RQQGDNVTVEANGGHLNISNSRFGVDKPDIVLGRQVRTAILSGNWFLKSKEIINHAGD
                     VVQESLSAVGAL"
BASE COUNT         1966 a         3038 c         3311 g         2001 t
ORIGIN      
        1 gagcatgcat gtcttgtagt ctttccccga ggtcttatac ttcatctggt cgacgaagcc
       61 gactttcttg gggccgcgaa acaaggctgc cgcccgacag ggcttgtgtc cctcctcgac
      121 agcctccggc cagtggccga gcccaaggat gtataattcg atggcatagt cgtagttctt
      181 tttgctgaca agctcttggg ccctcgtgaa gcattccttg gccttggcga tctcgtcggg
      241 gccgaaagtc ggtcgtgttg gattggcagc gctcttagcc ataagtcctg tgtttgtcag
      301 gagatcttgg cccgatacgg cgtcgcgcag caggagcctt cacggtgcca cgccatatct
      361 ggtgcccaag gccgcccgag tcccgcccga gcatgagctc cgtcgcgcct ttggacaggg
      421 cgctaacgcg tcgttcacgc tgagtgtaag actgctaact gagatagtat cacgaccggc
      481 ccacgggtca aggcaagtcg ccactggcgg ctggccgtcg cgtggatgaa gcactataat
      541 ttgtttagat cgcgctgcgg tgcgcgagaa cagccttggc gacctttgtc acggtggccg
      601 atacaggaaa acggagatgc ttgtcgaaac cctgccgtcc atgatcgtcc gatatcctga
      661 tcctcggttg cgtcggaagt gcgagccgat cagcgttttt gacgattctc tggccgctct
      721 cgtcgagagg atgttccaga tcatgctgag ggaccgcgga gtggggttgg ccgcaccgca
      781 ggtcggtctt tcgtgctcgc tctttgtagc caaccccacg ggcaatcccc aggacaacgc
      841 ggtctacatc aaccccaaat tgtctgagtt ggtcgggtcg gtggagggag aggaggggtg
      901 cctgtcgttc ccggatgtga gggcaaacat ccgacgagcc aggcgatgcc gcatccaggc
      961 ccaggatttg gcaggcaatc cgatcgacca gattggcgag gatcttgttg cccgtatctg
     1021 gcagcacgaa atggatcacc ttgacggtcg gctgatcgtc gaccacatga atttcgccga
     1081 caggctggcc aacaagaagt cgttgagcga cctcgaagcc tcttaccgca agcgggccaa
     1141 gcggaggtag atggtgttct cgccggcgcc tgaggcgcaa ctccgtaact ccgcatccgg
     1201 acacagtctt tctgatcgat atcttcgtcg gcgccgaaaa cgtcgggtat catcaacaca
     1261 ggaatgtcgc acaacgtcac tgatgttgcc ggaccgctat cgttggaccg gatgcgcatc
     1321 atagggacgg cggagtccgc agagcagtcg aaccgagtcg aatgccggca gagcggcggt
     1381 agactgccgt cgtcggtcct caaggaacgt ttatctggta ttgtattggc cgctgacgat
     1441 ctgggttcag gacggtctgc cgcgggcacg gtatcggaca cccagggctt atttgcctgt
     1501 taccggcagg atgatgcgtg acggatgctc cctgccgcag aacagctggt tgtgggcctt
     1561 gagcatcgta cccatcggcc aggccggcca gccggtgttc gggttgacat cgaagcgagg
     1621 gtagttgctg ccagtcacgg ccactcgaat acggtgcccc ttgttgaaga ccatgcttgt
     1681 cgaccacaga tccacctcga tttcggtcgg ctgacccggc tgcagcagag tcagccggtc
     1741 gaagcccttg cgatagcggc agcgggccag gccgtcggcg atgttcatgc tgcggccgtc
     1801 ggggtagacg tcagtgagct tgaccgcgaa atcggtgtcg atccggtcgc tggccacgaa
     1861 gagcctggcg gtcagtctcc cggttacctc cagtggctcg gccagtggtt cgctggtgaa
     1921 ggtgacaacg tccggtcgtt gctcaatatc gcgctggtcg tatactccgg gagcaacctt
     1981 tcgcttggtt ccgtattcgt tccacgacag caaaaggcat ccgcctctgg tcgggaccgg
     2041 gttgagcgga tcatggatga aggctacctg ctcgctggtc gtttccgggg ccttgcgcga
     2101 caggctgttg tcagaataga ggtagaacgg ggttggcttg gcgggcacgg gccagttatc
     2161 ggccgtatgc cactggttgc ctggcgcatc ctcttccccc acggctccca tggtgtagta
     2221 ctggacggcc ggtacctttt ccacgccggt gtcgatgcct ttgaggtgaa agtcaaacca
     2281 ctcgtcggcg ccacaactga acggcgtttt cagtgagttg gcaggaaaag tcagttcgcc
     2341 gatgggcgag gccttggctg ccaggggcag atcggaatcg cgtttgggtc ctccgtgccc
     2401 ccaggggccg atgatcagct tctgtttgcc cttggcccct ggtccgccgt cgttatggcg
     2461 gctgacgaag ccgtcgattg tgccttggag aaagacgtca taccagccgc cggaatggac
     2521 ggccggaacc ttgatccgat gagattcggc gacagagtcc agcttgcgcc agtgtccgtc
     2581 ataaaaagga tgcaagcagg tgagccacaa gttgtccggg tggaagcccg cgccggtcag
     2641 ccagccgtcg acgagggcgg cccgcaatgc gccgctgacg tatgaggcat ggtgatacag
     2701 gctgggagct gcgaccatga tgtattgggc gaccaatccc ggcgggtccg tcggagcgag
     2761 catacattga gtgatgccca tggccgaggc tccctcggtg ccgattttcc cgttgcacca
     2821 gggctggtcc aggatccact tgatcgtgtc gagaccgtcg cggttgtcgc cccacccgca
     2881 gtcaatgaag gccaagtcct tgccctcaga atcgaaccgg ccgcgcatat cctggatgac
     2941 catgacataa ccccggtccg ccttggcgcc atattcacgg ttgaagcggg ttcgcccgta
     3001 gggcgtgcgc accacccgga ccggccattg cgggccgccg gttgctggga ggtagacatc
     3061 ggtggccaga agagtcccgt ccgtcatggg aaccatggtc gtcagactgg ctttggggtc
     3121 ggctgcctgg ccggaggcag ccgccgccaa cgcggtgcaa agacacaatc ggatgggcaa
     3181 tagatgtcga cattgaggca tattggcaac caagccgttt ttccggcccg tcctctctgc
     3241 tgggactact gcggcggttc ctgggcaaca gcgtggattc tagcggtccg ggtgaaacct
     3301 cacaacgagt acagaggctg ctgctgttct cgctgggtcg gtggtgcacc ggcgggcaag
     3361 ccgtcagtgc cacccgcccg gcgtgcagcc gaaaagggcg tcagggcgtg gcgacacctg
     3421 tgaaaccgct gaaggcccaa cttttcggtt ggcaccgtgt ctaccggttg gcgagaagag
     3481 ttcaatgtcg tatactttgg gagcagcagg ggtgaagttg gtcgaggcgt tgtcaggtcc
     3541 gagcgagccc atgatccagg cgatgctcaa ttatctgcgg agcatattca cctccgaggc
     3601 gtatccgcct tttcaggtat tgctggagat ggcgctgatc ggcacggtgg tctacagcgt
     3661 cctgcggttt ctgcatggaa cgcgcggggc ccgactgctg caaggcctga tcattcttct
     3721 ggtggtcgga ttcctgcttg tcaacgtgat ggccgagttt ctcgggctcg accgattgac
     3781 ggtgttgtat cagccctttc tgttgtcggt attgctggca accctcgtcg tcttccagcc
     3841 ggagctgcgg cgaggtctca tgcgcctggg ggagacccgt tggcgtcgga tcagccaatc
     3901 ggaaatcgac cgcgtggctg ttccagtcgc ggccgcctgc acgcagcttt cgaagaacaa
     3961 gattggagcg ttgatcgcca ttgaaagaga tgtcggactc tcggctattg cggaagaggg
     4021 ggtgcgcctc gatgcccgct tgtcggccga actgctcaat accattttct ggccgggctc
     4081 ggccttgcac gatctcgggg tggtgattca aaacgggcgg atcgccgcgg cagcctgtga
     4141 gtttccgctg gtcgatgccg agggggttga ccactccatg ggcagccggc atcgggcggc
     4201 gattggcctc agtctcgaat cggacgccat tgtggtcgtg gtcagcgaag agaccggttc
     4261 gatctcaatt gccgaacgag ggcgtctgta tccccatatc cgcccggccg atctttacga
     4321 caccatctgc cgccatttga gtcatacccg ggtgccgctc gtgcctcgag aggaggttgt
     4381 accagaaccc gagccggaac cggtgccgag tcctgcggtc tcggagggta aggtcggcga
     4441 tgaggttccg gtgggtgaga cgctcgacca ggaggttgcg gccgaccccg ccaagtcacc
     4501 gcaccatgaa ccggcggagg cccctgcatg aaaacccagc tccgcctcat cgcatcgacg
     4561 attgttctta cggtgttgat ctggatctat gccaatcagg ccagccacag caccacgacg
     4621 ctgcttgttc cggtcaagct tgctctcgcc gagaccggcg gctcctgggc tctgcgtgtt
     4681 gccgacgccc cggcggacca acccaatacc cgacaggtga agctgactgt ccgcggcccc
     4741 aagcaggcga tccgcatgct ggaaagtgag actggccagg ggcgttttgc attgaccgtc
     4801 aagctggccg agcagcttac cccgggcaag accaatagtc gcaatctcta tgccgacctg
     4861 agcggttctc ccgatctgtt gcaacgagga ttgaatctcc agtcggttat gcccgaggtc
     4921 atcgagttcg aggtggatcg ttacatcagg gtgccggtcg agttcaagcc ggaggcgggg
     4981 gctttcaaga gcgcgctggt cggccagccg acccttgcgg agaaggtcac cgcccgcgtg
     5041 ctggagagca aggtgggcaa ggatttcgcc gtttatccgc tggtcctgcc gctggaagat
     5101 gccatcaggg aggccttcga caagaggcga accgaagagg cggccgggcc gctgagtctt
     5161 catttccccg tgcagttgca gtcgctgcga ctcgcggttg atgccgactt cgagccgccg
     5221 gttgttcacg tgaccgtcga actgaaagac cagtcgagca tcgaacgtaa aacggtccgc
     5281 cctttgtcgg tgatggtcag gacgcaggat ttcttcgagg gctatgaaat cgaatgggaa
     5341 gacaagacgg gggcgcaatt cacccagtcg atcaactgcc gtgtgccggt gtccaagctg
     5401 gacgcctttc gccagatcga gagcaccggc attctggcct acgtgatcat cgacagcagt
     5461 gacctgcccc cggaaccgac gactgccgga ccgacggcta ccactgttcc cgctcccacg
     5521 accagcgcga cctatagacc caagcaggtg cacttcgttt tcccgcccgg tttcgaggac
     5581 atcaagcccg ttggcccgcc tccgacggtc aatcttcgcg tcaacaggaa gacgaccgac
     5641 gcaaccggcc tgccgacgcc ggcggcgtcg tgacggatcg aatcgagctg ctcttgctcc
     5701 gtgacaagtt cccgcggacg gatgccgtac tcttggaagc agagcactgg cggacaagcc
     5761 gcctatgacg gcgtgtggag gacaagccgg cggtggaagc cggcgccgtg ccaatccggc
     5821 ctgtaaagcg atataatccc aggccatgag caacgaagca ctagaacagt atgcgttacg
     5881 gctggcccgg gatgcccggg cagccggtcg aaaactggcc ctgacttcgg gcaagcagcg
     5941 gaacgaagcc ttgcgggcga tggctgcggc cctgcgcgag cagaccgatg ccctggtggc
     6001 ggagaacgcc aaggatctgg ccgccgcgga ggctaacggc ctgacctcgg cgatgatcga
     6061 ccggctcaag ctcgacgcag ggcgagtggc caagatggcc caggcggtcc tggagattgc
     6121 cgatcagccc gatccggtgg gtgagaccat cgaagggcgt atgcgccccg acggcttcag
     6181 ggtggagaag cgtcgtgtgc cgctgggcgt ggtggcgatc atctatgagg cgcggccgaa
     6241 cgtgacttcc gacgcggccg ccttgtgcat caagagcgcc aacgcctgca tcctgcgcgg
     6301 cggcaaggaa tcatttcact cgaacgtggc gatcggctcg gcgatctcgt ctgccttggc
     6361 caaggtcgat ctgccggccg aggcggtgca gatcgtcaac accaccgacc gcaacctcgt
     6421 gcccatcctg ctcaagcaga acgagagcat tgacctggtt atccctcgcg ggggcgagtc
     6481 tctgatccgt gcggtcgtcg agcagtcgcg cattccggtc attaagcatt acaccggcaa
     6541 ctgccatgta tatgtcgact ccggctgccc ggccgagatg gccgagagcg tggtcctgaa
     6601 tgccaagatc cagcgaccgg gtgtgtgcaa cgcggccgag acgattctgt tccatcggga
     6661 cacggccgac acgctgttgc ctgtgatcgg taggaagctg atggatgcga aggtcgaggt
     6721 gcgcggctgc gccgaaacct gcaagcggct ggccggctgc aaacccgcga ccgagcagga
     6781 ctggtacact gaatacctcg acctggtcgt tgcggtgaag gttgtggact cgctgcagga
     6841 tgcgatcgac cacattaacc aatacggctc acatcatact gacgccattc tcacgcccga
     6901 tattcgctcg gcagaggctt ttgttgccgg agtggattcc ggcaacgtca tggttaattg
     6961 ctcgacccgc ttgagcgatg ggggggtata tggtctgggc gccgagatcg gtatcagcac
     7021 cgacaagctc cacgcccgcg gtcccatggg tgctgccgac ctgaccacgt acaagtgggt
     7081 ggtgaccggc aacggccaca ttcgccactg agaaccagga ggcaagtatg aaaagaaaag
     7141 tacactcggc tctgagcgtg ttcttagcaa tcgcttcgac gtcttgtgca aaagcagccg
     7201 tcggtcccgc ggcggttctc cgcgaggcac cgctgatccg catgccgggg gttcccaagc
     7261 cggacgcgcc gcaggggggc gccgtcgatt gcaacagtcc ctgccattgg gacggcgaca
     7321 cgatgtacat tttctcgtcg gagggccgtc cttttcggtc ttcggggccc gatctgatgc
     7381 acttgaaccc ggagtccgaa cgcagcacct acgacaacga gaagggctac aacggcgggc
     7441 ggtggataga ggccacgcac aaagcggagg acggcaagct ctacggctgg tatcaccgcg
     7501 agcccggggg cgtctgcccg ggcaaccccc tgaccgcccc ggagatcgga gcggtggtct
     7561 cgaccgacaa cggcatgaac tggcgcgacc tgggcatcat cctgaaggcc ccggacgact
     7621 cgatcttctg tgacacgccc aacaagtatt tcgccggcgg caacggcgac ttctccgtca
     7681 tcctggacca acaggaggag tatttatatt tcttggtcag tacttatcac aaggatccga
     7741 aggagcaggg cgtggccatc gcaaggatgc gatacgccga tcgcgatggg cccgtcggca
     7801 aagtcttcaa atggcaccgt gggcagtgga aggagccggg gttgggtgga cgcgtcacac
     7861 cgatcttcgg tccgacgatc gattggcaca aaccgcgcgc gaacgttttc tggggccctt
     7921 caatccactg gaatacgcac ctggagcgtt atgtgatgtt gttgaacctc gcccgggaca
     7981 aggactggac gcaagagggg atctacgtca gcatcaatcg ggacattgtc aatccggagg
     8041 gttggagcaa gccggagaag attctggacg ctggtcgact ggaggcgtcg aagtggtatc
     8101 cgcaggtgat tgggacgaat gccgccggtc gtgagacgga caagctggca ggccgtgtcg
     8161 cccggctgtt cgtggccggc cggtcgaggt gggagatcat cttcctcagg ccgggggagg
     8221 ctcgcccggc cggggtgatg gagaatccgg acaaggccgt ccgcacgcca agcccggcga
     8281 agccaacggc caagaagaac accgttacaa agactgcccc aaagaagaaa tgaagcattt
     8341 gtgtttggtc ggcaggacgg gcaccgctca agaccgttat ctcatgagaa agttgccagg
     8401 catgcgaaag cgctcaacaa cttggtttat ggcgacattt gttgcagcgg tacctgtgat
     8461 ggctcaggca gccgcggtcg cgagcacccg gccgtttgat gacgcgccgg ccggcgactc
     8521 ggccgccatt cagaagctcc tcgatgcagc ggctcccaag ggcggcacgg tggacctggg
     8581 agtcgggcgg tttcgactgg atcgacccat catcgttcct gagggcgtat gccttcaggg
     8641 cacttggcag ggtcctcatc acgctcaact tgccaaaggc acggtgttcc tcgtctacgc
     8701 cgatgtcggc cgggaggatg gcccgccgct gatcacgctc agcccatcaa gtacggtcaa
     8761 agggattacc ttcttttatc ccgagcagcg gatccccggg accaaggcct acccgtggac
     8821 catccaggga cgcggcatgc acaacagcgt aatcgactgt accttcgtca acgcatacca
     8881 ggcgatggat tttgccaccc acgccaatga attgcactat gttcgcaact gcttcgggtg
     8941 cccgctcaag gtcggaatcc gcgtcgaccg ctgcacggac attgggcgga tcgagaatgt
     9001 ccatttcaac ccgcactatt gggccagagc cgaagctccc ggcgtacccc aatggcccga
     9061 tctcagccgc tatctgtggg aaaacctgat tgcatttgac ttcgcccgga ccgactggga
     9121 gtacgtgctc aacaccttct gctatggagc gaagatcggc tatctcttcc gtcagacccg
     9181 gcacggcacg gtgaacggca acttccttgg catcggcgcc gactggtgcg agcgggcggt
     9241 tgtcgtcgag gccagccagc cgccgggcct actcatcacc aacggcgagt tcgtcggcgg
     9301 agaaggctcg gtggtgatga tggatgtgac tgcttcacac gcgggcgtcg ttcaactcag
     9361 taactgcgca ttctggggcc cgtgcccgac cgctgtccgc gtcgacggaa aaggcagcgt
     9421 cagcctcagc cagtgcaact tcctcaatca cggcaagcct cggcagcagg gcgataacgt
     9481 gaccgtcgag gctaacgggg gacacctgaa catcagcaac tcgcgcttcg gggtcgacaa
     9541 gccggacatc gtgctgggcc gccaggtgcg caccgcgatt ctctcgggca actggttcct
     9601 caagagcaaa gagatcatca accatgccgg cgacgtggtt caggaaagcc tgagcgcggt
     9661 cggggccttg tgatgtgaag gcgcataaaa aagaggatcc aggctcgccg tcacccggac
     9721 cctccaagcc aggctgtccg ccctccaggg cggagaccca cagcctctct tcggtatttc
     9781 ggttacacgg gggggtgaga taacttgttt ttcgagattg ggccgggtct gagcagaaga
     9841 ggggacgcct ttgactgccg cccaggcgaa gcccggtagg gtcgccatgt ggcagatctt
     9901 cctgggagtg tgcccaccaa aagcgggcga ccaggtccgg accggcaaca cctggcctgg
     9961 agcccacgac cgcacagtcc gagacgcgca aaccacgtta ccaacggggt ttgcgaattg
    10021 caggatatgg ggactttgtc gcggttttga ccggtttgtg gcccgttgcg cggtgtacaa
    10081 ggtgcagcac cgggcgcggg attccgccat cggacttctc tggatcgtgt tttttcggta
    10141 agcctggatg aatcgtgacg cgtctgatat tgtccgcggc cggtgcgagc gtgcatggag
    10201 gacggcggat gagaaccgga acgacgcaat cacccgcgaa cgaggctttt tgccggagcg
    10261 gcggctggcc ttccaccagg ccgagagcgg cccgatcatg gagcagctcc gggcct
//
LOCUS       DAOTPW010000207        10314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6055_length_10314_cov_5.596257, whole genome shotgun sequence.
ACCESSION   DAOTPW010000207 DAOTPW010000000
VERSION     DAOTPW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6055_length_10314_cov_5.596257"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="P5316_16530"
     CDS             <1..323
                     /locus_tag="P5316_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRR86619.1"
                     /translation="TTYSNRRNLCTFRTTLANAIEHACVLCRNDTITARDLPASVRAA
                     SISHLACDDSPVVPLAVAERALIARALQAAGGNKSLAAAMLQIDRRRLYRKIRAYGLH
                     INRQ"
     gene            818..1348
                     /locus_tag="P5316_16535"
     CDS             818..1348
                     /locus_tag="P5316_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86620.1"
                     /translation="MSRASEPVLVVVLDRVVTPALARLLMRRGWPLLRATSASQAVQR
                     IRDAEPETLVIQVSLDSAETLDLLDRVRRLRPGMPVVTVATYEGHELERTVRAVGVSC
                     YMASADPELIEQAVLAMVGATTPSGASLAHGRYRPQQAEPVTQVVFSLPKTVGAAAGA
                     APERGTRKKKLWPEVA"
     gene            1387..1566
                     /locus_tag="P5316_16540"
     CDS             1387..1566
                     /locus_tag="P5316_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86621.1"
                     /translation="MSLTEAQKKTLSRCSGEHLLLLRILTGSRLKKVIDEELDRRAGV
                     CVRRLRQTARVRRAA"
     gene            1605..1802
                     /locus_tag="P5316_16545"
     CDS             1605..1802
                     /locus_tag="P5316_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86622.1"
                     /translation="MDKRLRKWMRRMDDDQLCALSELVAAEIDRRSERRFPRAYVRST
                     YMSDMVRGERKAEPRRVRWAA"
     gene            1854..2057
                     /locus_tag="P5316_16550"
     CDS             1854..2057
                     /locus_tag="P5316_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86623.1"
                     /translation="MSGNSGKTQTRREVPVLRLETAEPVRPLVVRSRKTRRGGLRLVL
                     GYWDWDPGHDPAERHSARLALAA"
     gene            2564..3802
                     /locus_tag="P5316_16555"
     CDS             2564..3802
                     /locus_tag="P5316_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86624.1"
                     /translation="MKTKMYLIAVGVMIGATSVMGVGLTVLPATHSGTNNANAASGYH
                     IQGMTDDGAYVVGTAYNPGGPYDGANGILIIKVSDLTTTLLQPKAGTSGRGIAYTSAG
                     LMVAGAVGSNSALGRAYDAGSPPSTNYYLDTMNGTTSEVKVAQMNCLSIDAATGNGWL
                     VGERHNGKEAVAYKYVNGVAQNKVGTNPRAFWEKQGTGDTVLNGVSNTGLAVGTDNGG
                     KRAIYADVANNGDVTPIPQHPNSTGEGQGNGMSAGGLYASGYMKPLALVSDDSLHAFL
                     WKVGSEYSVELLPAGGDIDGVQQSNSFDVSDNGTAVGFTWNGKDRAGNSFTGYRATVW
                     ENGNTTGVLVQDILTSQGVDLSAWQYLERCISVTPDGLTIAGRGVLADGTYRGFVAVI
                     PEPAGVVLLGLGSLLLRRRR"
     gene            3927..4496
                     /locus_tag="P5316_16560"
     CDS             3927..4496
                     /locus_tag="P5316_16560"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86625.1"
                     /translation="MKKLVTICAILCMAATAAQAVFIVESRDAAGNLTPAPAYAEFGV
                     WANAGAKSTAEGLIGVGSRYGSTYRSLAGLKEATFTVDLPVAGEYEVFVTWGAGANRR
                     ANIEHRIVHAGGITAVLVDQRATANEWVSLGTYTFNAGPASVTVSNAWVDLSGSMYAD
                     AVKWVPEPGAMALLVLGSLGGVVLRRRRA"
     gene            4951..5619
                     /locus_tag="P5316_16565"
     CDS             4951..5619
                     /locus_tag="P5316_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86626.1"
                     /translation="MNKVFLTCGILLTATSAFANLIQNGSFETGDISGWTYWHAPWCV
                     NCQAVVQTGSGTMVDGQSRARLQISNGEGSFGLYQVVAVPTDKEVTLEAWVRATSNNV
                     NWAEVLLYDYAVTDINDIDSGEKSAPYMIWKRDSWGGVSGLPGGYLPTVATSGGPGDS
                     WEMTTKTLTSVPSGVVTVAFKWGRSGGGFSGSMYLDAITLTPEPAMMLLLGLPALFLR
                     RRRL"
     gene            5871..6656
                     /locus_tag="P5316_16570"
     CDS             5871..6656
                     /locus_tag="P5316_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86627.1"
                     /translation="MSKALLLLALPGTAVLVVAGLTAGPALADWDVGDPYKMHYPQLP
                     DPSGWDVKASYSKVLADDWLCTQTGPVSDLHFWGSWYKDEFRDAGPPVPSFEWFTISI
                     YADIPDPDDTGPEFSKPGEQLWTRVFYPGEVAVRQWGTQDQGWYDPNTSEYEEYNHKL
                     YFQYNIENIVDPFIQQEGTIYWLAISMECNYGTWGWKTSADHWNDDGVWRDEEPLQPT
                     DWAELHDPLTGVSLDLAFVITPEPAAVVFLALGSLLLRRRMRC"
     gene            6825..7595
                     /locus_tag="P5316_16575"
     CDS             6825..7595
                     /locus_tag="P5316_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86628.1"
                     /translation="MRNGFLALSALPSLAVLVVAALMAGPVLADWQIGDPCKMHYAQL
                     PDPNGWDVKAKYTKVLADDWLCTETGPVEDVHFWGSWYNEVVGDIRWYQISIHADIPD
                     PDGPGPEFSKPGELLWLRIFYPPEVQVRPWGTGQQGWYDPYNQFWVENNHINTWQYNI
                     NTIPDPFIQEKGKTYWLDVSVMCSYGDWGWKTSISPHWGDDAVWSAYIPGEYTPWQEV
                     RDPTTGESLDMAFVITPEPGVVVILALGSLLLRRRRRA"
     gene            8595..9143
                     /locus_tag="P5316_16580"
     CDS             8595..9143
                     /locus_tag="P5316_16580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86629.1"
                     /translation="MTSLVGDLATNPNLLDLDDKHNLSASGGRDERDYDVYASDPGTV
                     VSPPFGGYDTAYIWFDRDGVDPWQDTKGANTGGTYNITIVYHAITPTLGSMIATINGN
                     GVDIVNQLIRVEGTYYPAGLSFTGDMTQMQVFAGAWWTSGAGGFVSVSDITASGTLIP
                     EPATLSLLTLGGLLLRRRRRCS"
     gene            9679..10302
                     /locus_tag="P5316_16585"
     CDS             9679..10302
                     /locus_tag="P5316_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86630.1"
                     /translation="MRRTTIFGCVLTVVLLIPATVWADAPPGTIVYIEDEFLPDQDMN
                     GWTTYGDRTMSNPAGNGFSLFNPTYRTDDVDGIAGIQEGHWGGRSFGMYKRFYDEYVP
                     NGEKHIQAWVKMMSCDWNWNQTPNALTVRLGKDPLGGTDPNAPSVIWTEPYWGDWNRL
                     ELIQPCVEGWETIFIQVNGSFSGYYAIYVDTVEVCQRRSESAEISPV"
BASE COUNT         2143 a         2826 c         3490 g         1855 t
ORIGIN      
        1 agactacata ttcaaaccgg cgaaatctct gcactttcag aacgacgctg gcaaacgcca
       61 tcgagcacgc ctgtgtcctt tgccgcaacg acacgatcac cgcccgcgac ctgccggcgt
      121 cggtccgggc cgcttcgatt tcgcatctgg catgtgacga ttctccggta gtccctcttg
      181 cagtggccga acgagccctt atcgctcgcg ccttgcaggc ggcgggcggc aacaagtctc
      241 ttgccgccgc gatgctgcag atcgatcggc gcaggctgta tcgcaagatc cgggcctacg
      301 gcctgcacat caaccgtcaa tagcccaagg cggaccgcct ttttgcgggc tgcgagccaa
      361 gcgctctgcg atccagaaac atgcgcgcaa gccgcacaga tgtctggccg taaggttctg
      421 acccccacgg cgttatccat tgcacgatcg gcgttcctgg cacgcgttgt gcgcgcctgc
      481 tgtaccacaa cggcacagtg agccattccg gtacaaatgt tcgctaagtg ccgccggcgc
      541 aggagacggc ggcgcacgga cccgccatgg cggtaccaca atggtacatt cctggcaaag
      601 cctctctctc gttcattccg ctcaaaggcc ttgtggttgc tcagcttgcg cgccgcccgg
      661 ttgcgcggca cgcctcctgc cgggttggcc catgtcgcgc cggatgcgcg ctcaaaacgg
      721 gacggccagg cgttcagcgt ggagggcccg cgcgcacggt tgggcgccgc ttgtgtccgg
      781 aagggtcgcg cagcaggcgt cgacaggaga gaattagatg tcccgagcaa gtgaacccgt
      841 cctggttgtg gttctggacc gcgtggtgac cccggcgctg gcccgtcttc tcatgcgccg
      901 ggggtggccg ctgctgcgcg caacgagtgc aagccaggcg gtgcagagaa tccgcgacgc
      961 agagcccgag acgctggtga tacaggtttc gctggactcg gcggagaccc tggacttgct
     1021 cgatcgcgtg cgccggctgc gtccgggcat gccggtggtg acggtcgcga cgtacgaggg
     1081 ccacgagctc gagcggacgg ttcgcgcggt cggcgtgagc tgttatatgg cctcggccga
     1141 cccggaactc atcgagcagg ccgtgctggc aatggtcggc gcgaccacgc cttccggagc
     1201 gagcctcgcc cacggccggt acaggccgca acaggcggag ccggttaccc aggtggtgtt
     1261 ctctcttccc aagacggtgg gcgccgcggc cggcgccgcc cccgaacgcg ggacaaggaa
     1321 gaagaagtta tggccagagg tggcctgacg aggtgtgttc gtgattcatg acgaaaggaa
     1381 agaatcatgt ctctgacaga agctcagaag aaaacgctca gccgatgcag cggtgaacat
     1441 ctgctgctgt tgcgcatcct gaccggatcc cgcctcaaaa aggtaatcga tgaggagctt
     1501 gatcgccgtg ccggcgtctg cgtccgtcgt cttcgtcaga cggcgcgcgt ccgccgggcg
     1561 gcgtgagtga ccgaggagat ctgcgagaga taaggagttc tgccatggac aagcgattgc
     1621 gcaaatggat gaggcgaatg gatgacgacc agctctgcgc actgtccgag ctggttgcgg
     1681 ccgagatcga tcggcgcagc gagcgccggt ttccgcgggc gtatgtccgc agcacgtaca
     1741 tgagcgacat ggtgcgcggc gagcgaaagg ccgaaccgcg gcgcgtgagg tgggcggcct
     1801 gatcggaaga gtcggcgggg caacccgccc cggcaaacga ggtggagggc gtcatgtctg
     1861 gaaacagcgg caaaacgcag acgcgtcggg aagttccggt gctgcggctg gaaaccgcgg
     1921 aaccggtgcg gcccctggtg gtgcgatcac ggaaaacgcg ccggggcggg ctgaggctgg
     1981 ttctgggtta ctgggactgg gatccggggc acgacccggc ggagcggcat tcggccaggt
     2041 tggccctggc cgcctgaagc cgggccccgg gaacacccgc ggcgcgtcca caaccaacaa
     2101 gccctggtgt gggaacagat catggttttt ctgggaggaa tgacaagacg aaacgcggcc
     2161 ccgacaaggg ccgggtcgtg ggttgccgac ccgcggccgt tggacgcaaa catcagcggt
     2221 gattcaagag aaggagaaga gaagatgacc cgggatcacg gccgcaagca ccggtcaagg
     2281 aggaacggga gaccgccccg ggacaaaagg gtgtgcgaat gcacgccggg caacatcgcg
     2341 gcgtgcgggg gttacgccaa acgggccgct cgcgtgagcg gccggcatgc cggagggcat
     2401 gcgaaaaagt gaatcagtag tgccgtgcac ggccggacga ggccgcggca cggccagact
     2461 cgcccgcaag ggcgaagaag cgcccgagat gactcgaatg aagaaactga ttggtctcgc
     2521 acatcttcgg attcgaccgg gcgcaagagg agtacgaaca acgatgaaga caaagatgta
     2581 tttgattgcc gtgggggtca tgataggggc gacgagcgtc atgggtgttg gtctcacggt
     2641 gctcccggcg acgcactcgg gcacgaataa cgcgaatgcc gcctctgggt atcacatcca
     2701 gggcatgacc gacgacgggg cctacgtagt tggcactgcg tacaatcccg gaggacccta
     2761 cgatggtgcc aacggcattc tgataatcaa agtcagcgac ttgacgacta ctctgctcca
     2821 acccaaggca gggaccagtg gtcgcggcat tgcctacacc tccgccgggt tgatggttgc
     2881 cggtgctgtc ggttccaact ctgcccttgg ccgagcctac gatgcgggct caccgccatc
     2941 taccaactat tatcttgaca cgatgaacgg tactacgagc gaggtcaagg ttgcccagat
     3001 gaactgtctg tccatcgacg ctgccaccgg caacggctgg cttgtcggag agcggcacaa
     3061 cggtaaagaa gcggtggcct acaagtacgt caacggcgtc gcgcaaaaca aggttggtac
     3121 caatcctcgg gcgttttggg aaaagcaagg gacaggcgac acagtcctga acggcgtttc
     3181 taacaccgga ctggcggttg ggaccgacaa cggagggaag cgtgcgatct atgcagatgt
     3241 tgccaacaat ggcgatgtta ccccaatccc gcagcatcct aattccacgg gcgagggcca
     3301 gggtaacggc atgtccgctg gcggcttgta cgcgtcaggg tacatgaagc cattggcttt
     3361 ggtgagcgat gacagcctcc atgcttttct ctggaaagtg gggtctgaat acagtgttga
     3421 gcttttgcct gccggcggtg acatcgatgg agttcagcaa tctaacagct tcgatgtctc
     3481 ggataacggc acggcggtcg gcttcacctg gaacgggaaa gaccgtgctg gcaactcgtt
     3541 taccggttac cgggcgaccg tttgggagaa cggcaacacg accggtgtat tggtccagga
     3601 tatccttacg tcccagggcg ttgatcttag tgcctggcaa tacctggagc gctgtataag
     3661 cgtgacaccc gatggtctga cgattgctgg ccgcggggtc cttgcagacg gaacctatcg
     3721 aggcttcgtg gctgtgattc ccgagccggc gggggtggtg ttgctgggct tggggagtct
     3781 tttgcttcga cggcggaggt agcggagcac gaacgtgagt ccttccgccg ggcggagaaa
     3841 ggcgtccggc gaggggacga gaaacgcaaa tagaggaata ttcttcacag tgaatagata
     3901 gaaggaaaac acaggagcat caaacaatga agaaactggt aacgatttgt gcaattctgt
     3961 gcatggccgc aacggcggcg caggcggtgt tcattgtaga gagccgtgat gctgccggca
     4021 atctgacccc agcgccagcc tacgctgagt tcggcgtgtg ggccaacgca ggcgcgaaga
     4081 gcaccgctga gggtcttata ggtgtcggtt ctcgctatgg cagcacctat cgttctctcg
     4141 ccggcctgaa ggaggccaca ttcaccgtgg acctgcctgt agccggtgaa tatgaggtgt
     4201 ttgtgacttg gggtgccgga gccaaccgca gggctaatat cgagcatcgg atagttcacg
     4261 cgggcggcat caccgcagtg ctggtggatc agcgtgcgac ggctaacgag tgggtttccc
     4321 tgggtaccta taccttcaat gcgggtcctg cgtcggtcac ggtcagcaat gcatgggtag
     4381 acctcagcgg ttcaatgtac gctgacgcgg tcaaatgggt tcccgagcct ggggcgatgg
     4441 cgttgctggt tttggggagc ctcggcggcg tggttctgcg tcgtcggaga gcctgaggat
     4501 ggtattcggt tcctcccgcc gggccggggg aggaaggatg cttgcccggc gggaggtccg
     4561 gccgggctac gggcatcagc cgccgggcag cctgattgag ggaagatgac caaacaggag
     4621 tgtggacaaa agagtgacaa gcctttgcag gaatatccat gaggttttcg ggaggaacga
     4681 caagacgata cggggccctg cgaaagggac ggggcctgat gtgtatccgc ggcggccggc
     4741 cgtggacgac agccaaaaca ggactgagaa gcagcctggg acaacggccg caaggactgg
     4801 ccacggacgg acaggaggcc accccgggca aaagggcgcg agaggcacgc cacggacaga
     4861 accgggcgtg cgagggttac gccgataagg ccgctcgcgt gagcggccgg caaacgaaaa
     4921 gcgaaagaaa cagtgtcata aggagctaag atgaataagg tttttctaac atgcggtatt
     4981 ttgctaacgg ccacatcggc ttttgccaac cttatacaga atggcagctt cgagaccggc
     5041 gatatcagcg gctggaccta ctggcacgct ccctggtgcg tgaactgcca agctgtcgtg
     5101 cagaccggta gtggaacgat ggtagatggg cagtcgcgag cccgcctgca gatcagcaac
     5161 ggtgagggca gcttcggcct ttaccaagtc gtggccgtgc caacagacaa ggaggtcacg
     5221 ctggaggcct gggtccgagc gacgagcaac aacgtcaatt gggccgaggt gctgctgtac
     5281 gactacgcgg tgacggacat caacgatatt gacagcggcg agaaatccgc tccgtacatg
     5341 atctggaagc gagattcgtg gggcggagtc tccggtttgc ctggcggcta cctgcctacc
     5401 gtggcaacga gcggtggacc gggcgactcg tgggaaatga ccacgaagac gctcacatcc
     5461 gtgccgtccg gggtggtcac cgttgccttt aagtggggtc gcagtggagg gggtttcagc
     5521 ggcagcatgt atctcgacgc cataacgctg acccccgagc cggcgatgat gctgttgctg
     5581 gggcttccgg cgttgtttct gcggcgtcgt cgcctttagg gggtcgtcgg gcaagggccc
     5641 aagtccttgt ccttgaggag aacgaggccg tcgtaccgcc gtggtgcggc ggcccacggg
     5701 caggagatca aaagagtacg tctgaaatgt gcgagttatc acactagaaa gaaccgctca
     5761 tgaccaggga gtgcacaagc atgaaaacgc tgacatgccc tccggaggca caggggcctc
     5821 ggcgccgaaa ggttcttttc cggcttggaa atgagcagga aggagtgcga atgagcaaag
     5881 cattactact attagcgttg cccgggacgg cggtgttggt cgtcgcgggc ctgacggccg
     5941 ggccggcgct ggccgactgg gatgtgggcg atccgtacaa gatgcactac ccgcagttgc
     6001 cggacccgag cggctgggac gtcaaggcga gctactcgaa ggtgctggcc gatgactggc
     6061 tatgcacgca gaccggtccc gtcagcgacc tgcacttctg ggggtcgtgg tataaggatg
     6121 agttccgaga tgcaggcccg cctgtaccgt ccttcgaatg gttcacgatc agcatttacg
     6181 cggacattcc cgacccggac gacactgggc cggaattcag caagccgggc gagcagctct
     6241 ggacgcgggt cttctatcct ggggaggtcg ccgtccgtca atggggaacc caggatcagg
     6301 ggtggtatga tcccaacacg tctgaatatg aggaatacaa ccacaagctg tacttccagt
     6361 acaacattga gaatattgtt gacccgttca tccagcagga aggaacgatc tactggctgg
     6421 ccatcagtat ggagtgcaac tatggcacgt ggggctggaa gacttcagcg gaccattgga
     6481 acgatgatgg cgtctggcgt gacgaggaac ccttgcaacc gacggactgg gcagaactgc
     6541 atgatccgct cacaggtgtt tcgctggacc tggccttcgt gatcacgccg gagccggcgg
     6601 cggtggtgtt tctggccctg gggagcctgc ttctgcgtcg gcggatgcgg tgctgacgga
     6661 aggcacagcg tggttcagca atgctgaacc gggaaaggaa atacgacgct ccgcatcaac
     6721 gagcgtccaa aacagtcgaa tacggacaat ctgctctgga ggcgggaagt ccttgctggc
     6781 agagtatttc ggtccggctt ggagatcagc agaaaggagt ttcaatgcga aacgggtttt
     6841 tagcgttgtc agcattgccc agcctggcgg tgctggtggt cgctgccctg atggccgggc
     6901 cggtgttggc tgactggcag atcggcgatc catgtaagat gcattatgcg cagttgccgg
     6961 atcccaacgg ctgggacgtc aaggccaaat acacgaaggt gctggccgac gactggctgt
     7021 gcaccgagac cggcccggtg gaggacgtac atttctgggg atcatggtac aacgaagtcg
     7081 tgggcgacat ccgctggtat cagatcagca tccacgcgga cattcccgac ccggacggcc
     7141 ctgggccgga attcagcaag ccgggcgagc tgctgtggct gcggatcttt taccccccgg
     7201 aagtgcaggt caggccctgg ggtacggggc aacaggggtg gtacgatcct tataatcagt
     7261 tctgggtcga gaacaatcac atcaacacct ggcagtataa catcaacacc atccccgacc
     7321 cgttcatcca ggaaaaggga aagacgtact ggctggatgt cagtgtgatg tgtagctacg
     7381 gcgactgggg ctggaaaacc tcgataagcc cgcattgggg cgacgacgcg gtctggtctg
     7441 cctatatccc cggcgaatac acgccctggc aggaagtgcg cgatccgacg acaggtgagt
     7501 cgctggacat ggccttcgtg atcacgccgg agcctggggt ggtggtgata ctggcgctgg
     7561 gcagcctgct tttgcggcgt cgcaggcgag catagctttc ctccagtgtc cccccgccgg
     7621 ggccgacagg tgcccggcgg gggggcgacc cgcatcgagg cgtgagtgcc cggtgtcgtg
     7681 gcggaaaaag cggcggggcg acccgcccga gtgaacgcag cagagggtgt catgtttggg
     7741 aacaagaaca caacgcagct ccgtcgagaa gttcgggtat tgcctccggt gcttgcgaac
     7801 ctgatccggc cgttgcgagg acgaggaacg cggcgaggca ggctgaggct ggttcacggc
     7861 cggtgggatc cggagcccga cccggcggag cgacattcgg ccaggctggc cctggctgcg
     7921 tgacagaccg gcccgaccgc tctgaagccg acggggcgtc ctttgacgcg ttcgcgccga
     7981 tgaagggttt gtgagaatga cgcatgagta ttcctggagg aacgacaaga cgagacgcag
     8041 ccccggcaaa catgggttgc aggcacgccg gagacacaac gcggcgcgcg aaggagatgc
     8101 cagcaagacc gctcgtgtga gcggccggca aacgagaaat caaagaaaag gcgccgtgca
     8161 acggccgcaa aaggccgcgg cgcggccgac tgactcggag gaggagtacg tgaagaatgc
     8221 ggtggaccgt cgcatgtgcg gcggcctgga gttgaagtga attcagaaac gtaacaacag
     8281 gagtctttgt gcacgtgatg agaacaacga cgataatgat ttgcgtatgt ggtatgttgg
     8341 cttgcgccac gcaggccgag acgattcatt atggaggtgt gaccttttat cctccgtatt
     8401 ctgccggaca ttacagcgat gtttgggatc tgacgcggag cgatctcgta ttgacgtaca
     8461 gcattgattt aagcgcggtt acgcaaacgt cgtcctccga aacgccttac gtggaggttg
     8521 ggattcgtca ggtcggcgcc ggcgacttta acccgggtcc ttggaacaca tatcagggcg
     8581 gtgcaggtgg atggatgacc tcgctggtgg gcgacctggc cacaaatccc aacttgctcg
     8641 atctcgacga caagcacaat ctgagcgctt ccggcgggcg cgacgaaaga gactatgatg
     8701 tgtacgcgtc ggaccccggt acggttgtct cgccgccctt cggcggctac gatacggcgt
     8761 acatatggtt cgatcgggac ggtgtcgacc cctggcaaga taccaaggga gccaatacgg
     8821 gcggcactta caacattacc attgtctatc acgcgattac ccccacgttg ggctcgatga
     8881 tcgccacgat caacggtaac ggcgtcgaca tcgtaaacca gttgatccgc gtcgagggaa
     8941 cgtactatcc cgcgggcttg agcttcacag gcgacatgac gcagatgcag gttttcgctg
     9001 gtgcatggtg gacaagtggg gcagggggtt tcgtctccgt gtcggacatc acggcgagcg
     9061 ggactctcat tcccgaaccc gccacgctgt cgttgctaac cttgggcggg ctgcttctgc
     9121 ggcggcgtcg caggtgttca taagctctct ccctccagtc ccccccgccg gggcagggga
     9181 aaagcacccc ggcgaggggg ggctgggagc gcgagttgcg gccttgcagg cagcgggcgg
     9241 cacgatcgag gaggcgaggc gagagacagg cgtgtgggac cgggtttctg acaggcaggt
     9301 accggaccga ttcctttgat ggcacataga ttcttttgga ggaacgacaa gatggcatgc
     9361 ggccccgtca acaggacgga gtcagaggta ttgaggaatt catccgtggc cgatggccgc
     9421 gggcttcagc cggagcttgg ctgaggagca gcctgggata ccggccgccg gcactggtca
     9481 gggacgaaca ggaggccgcc ccgggtaaaa agggcatcaa agcacgccga ggagagaaca
     9541 cggcgtgttg gggttacgcc gataaggccg ctcgcgtgag cggtcgacaa acgagaaatc
     9601 gaaggagagg cgccgtgcga cggcctgaga agcttgaggc acggcgacac gcccgttaag
     9661 ggcagaggag tacgtgacat gaggcgaaca acaatattcg ggtgcgtgct gacagtagtg
     9721 ctgcttattc ctgcaacggt atgggctgat gcgccgccgg ggacaatcgt ctatatcgag
     9781 gacgagttcc tgccggacca ggatatgaat ggctggacga cctacggcga ccgaacgatg
     9841 agcaatccgg cgggcaatgg gttcagcttg ttcaatccta cgtaccgtac cgatgacgtg
     9901 gacggcatcg caggcatcca ggaaggccat tggggcggcc gcagtttcgg catgtataag
     9961 aggttctacg acgagtatgt ccccaacggc gaaaagcaca tccaggcgtg ggtgaaaatg
    10021 atgtcgtgcg actggaattg gaaccagacc cccaacgccc tgacggtccg cctgggcaag
    10081 gacccgctcg gcgggacgga tccgaatgcc ccgagcgtga tctggaccga gccgtactgg
    10141 ggcgactgga acagactcga gctgatccag ccttgcgtcg agggctggga gacgatcttc
    10201 atacaggtca acggcagctt cagcggttac tacgccattt atgtcgatac cgtcgaggtc
    10261 tgccagcgtc gttctgaaag tgcagagatt tcgccggttt gaatatgtag tctt
//
LOCUS       DAOTPW010000208        10297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6073_length_10297_cov_5.227592, whole genome shotgun sequence.
ACCESSION   DAOTPW010000208 DAOTPW010000000
VERSION     DAOTPW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6073_length_10297_cov_5.227592"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="P5316_16590"
     CDS             complement(<1..867)
                     /locus_tag="P5316_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86631.1"
                     /translation="MTGLREIFNLLADWAAKGGVDASLPSWWPNPPTLIAVLLVLGVI
                     LAFWGSRLLRLIYVLAFIVAGGFLGVTVARKTNVDLLIGLVIGAGLIGLIGHLLFRWW
                     VGVTAACCAALLVLTVAANQNAENLISALTEYHQQQKVLIVPPPPPETSAGPESRPSP
                     TLMIGQVAWALKAYVVDAFSYFRQTRQNVVAKVVIVAVLAWITGLGMGLALPRFTTIL
                     GTACIGVAMISLGLGMLLATYSPETLNSVMSKDKYVAAGLGIVLLVSMIVQARHRRPV
                     AQPSACPVPPPKA"
     gene            complement(949..1902)
                     /locus_tag="P5316_16595"
     CDS             complement(949..1902)
                     /locus_tag="P5316_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86632.1"
                     /translation="MSRSSQRRGDGNSADESTWSSLRANVRAFRPIVSAVFLVAFLVS
                     VVAGLERLERHVTQEPGQKTCIRVELDVPPDMDWVDKEGWRPRILSSVRVPQEPLGAD
                     ESLLQNIARQLLDSGWVSQVRRVVYTPDGTIRITCDCRRPIAMLLTEQPGNGRPIYVP
                     VDKHGYRLPETYVDISGCGAWIQILGVKAKPPEVGRPFEADDAKAGIKLAALIFQQSF
                     HSQVAAVDVTNHRGRVNKAKSHILIHPRAKKSEAFHWGSAIGEEIEEPSASEKLRMLA
                     KCFAPGSPQASIDLSVYSNAGIERAPDTTPKADALASRRKQ"
     gene            complement(1934..2893)
                     /locus_tag="P5316_16600"
     CDS             complement(1934..2893)
                     /locus_tag="P5316_16600"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRR86633.1"
                     /translation="MTQIADHVAFTPVNSKTVERRLRITVLSGGPGSEREVSLKSGRA
                     VAAALKSLGHDVYLADISPEDLSALDRPVDMVFIALHGAFGEDGQLQKILDDRRIRYC
                     GSGPQASAMAMNKALAKSRFMEVGVPTPLFEVVTPDNIEQVLAQWTPPSVVKPLCEGS
                     SVDCVIVHQGQDLEAPIRKEIEIYGQCMIERFVKGSELTVGVLGDEPLPPIQIRPKRE
                     FYNYEAKYLDNDTEYLFNIDLPATLLGEICDLSVRAHQSLGCRDFSRVDWIVEESTNR
                     PFALEVNTIPGFTDHSLLPKAAQQAGLGFSQLCQRIVELTYLR"
     gene            complement(2947..3894)
                     /gene="murB"
                     /locus_tag="P5316_16605"
     CDS             complement(2947..3894)
                     /gene="murB"
                     /locus_tag="P5316_16605"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRR86634.1"
                     /translation="MKTDFLSDLSGIVTQNEPLASHTCLRVGGPARWFARPRSIDHVR
                     ELVRRCNSEGVEFLPLGLGANLLVSDEGVDALVIRLGEPVFQSVDWGNEDARKRDKPV
                     TISVAAGTDMYRLTSDAVRSGLGGLERMAGIPGTIGGILRMNAGGRFGNICDVVHHVT
                     VVDAAGELRTLSRKEAGFRYRGSNLGGTVVCSAGLTLEPADPDQLKARFREIWEMKKN
                     TQPLADNSAGCVFKNPPGASAGGLIDQAGLKGRRVGGAVVSPRHANFIVTEAGATAQD
                     VLTLIGIIRREVADRFGVEFETEVQIWGRRRLRAGEPIR"
     gene            complement(3988..5526)
                     /gene="murC"
                     /locus_tag="P5316_16610"
     CDS             complement(3988..5526)
                     /gene="murC"
                     /locus_tag="P5316_16610"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRR86635.1"
                     /translation="MSHVAGANCEASNGLALDGPPTHLTKPDRPAAEDGVFNPVGLRV
                     HMIGIGGCGMRGAARVLMRAGARVSGSDRKESTALVLLSEQGANIHIGSKPGHVPDPC
                     DLVVHSAAVKESDPEMMEAVRRGLKVVKYSQLLGLLMSRKLGIAVGGTHGKSTTTAMI
                     AYVLREAGLDPSFVIGAGVEQLGGGSGVGDGEQFVVEACEYDRSFHNLRPRCAILLNV
                     DEDHLDYYRDIREIISSFHDFAALVPANGLLVVNGEDRNTMEAVAGAKATVETFGCQN
                     LFDWRGEIVEVRNGSYRFRVFRKGQVLTEITPGVPGRHNVMNALAATAVCFYCGVRPE
                     VIARALADFRGAHRRMTPRGSIGGVTVLDDYAHHPREIQATLKTVREYYNPNKLYVVF
                     QPHQHSRTRFLLNDFAKSFGAADVVVVPDIYFVRDSESERELIDSKDLVDLLRQQDRD
                     ARYEADFDSITRMLCEEVGPGDVVITMGAGNVWQVADSVLAGLAGAQQDGDPFVAMGD
                     IADH"
     gene            complement(5927..7123)
                     /locus_tag="P5316_16615"
     CDS             complement(5927..7123)
                     /locus_tag="P5316_16615"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRR86636.1"
                     /translation="MGFHAKGTNVSESRHWFVFAGGGTGGHLFPALAVVELLRERGLP
                     VDVSFFCTQRPIDQNVLDEAQIQAYPLSVLPFPTRPWAWPRFLWHWRQSVSVCRQAFQ
                     QRRPAVIVGAGGYASGPPVHVGLKMGIPTFLLNPDAVPGRANRHLGKRPDLAGVFAQW
                     DVTKASFPPTAPVYVTGCPVRKAFRSVCESAAKLNTLEAVRDKMGELKQSFSLDPSRP
                     MLLITGASQGARTINEAFIRLADQIASAGWQVLHLTGQADYERVAREYRRSSLSAAVL
                     PFTDRMAHAMLASDLIVSRAGASTLAEILAVGKPSILLPYPFHRDRHQWHNAEVLVKA
                     GAAILLDDLKDGEENARQIRPVLTDILNNASHRERMGAAACGLGRPQAAAKIAQHLCQ
                     SAGIDF"
     gene            complement(7181..7498)
                     /locus_tag="P5316_16620"
     CDS             complement(7181..7498)
                     /locus_tag="P5316_16620"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRR86637.1"
                     /translation="MICEELGIVGGIAVIGLLLTFLWLGRSVVFRCPDPVGRLLAFGI
                     VLMFGIQAAMNIAVVTASVPTKGIALPLVSAGGSGTVFLGGVVGVLASIARHSGSAPP
                     EKA"
     gene            complement(7495..8373)
                     /locus_tag="P5316_16625"
     CDS             complement(7495..8373)
                     /locus_tag="P5316_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRR86638.1"
                     /translation="MSDALTMEMDPALSETESLTAEERAHATSVWIVAVAGGLMAIGA
                     VMTFSASTSIDHPLVIWPVWETDAIRQLAFVTAGLLAMLLMTRVPYTWFSLGRGYLAL
                     ILLILALGVASLVFVPGLGVASHGAYRWLKIPGTTSRFQPSELVKGALPIFIATWTVY
                     RVDIRKFWTGLVPMGAVIALCAGTIGIEDFGTAALLALVAGGMLLVAGAKIWHLLLLT
                     LPAVPAFGYLIISKSHRVDRLTIFLDPFQDATGKGYQIIQSLCTIISGGWWGRGLGNG
                     FVKGYLPEAVTISSSR"
     gene            8722..9630
                     /locus_tag="P5316_16630"
     CDS             8722..9630
                     /locus_tag="P5316_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86639.1"
                     /translation="MSTLALLGILCVASYEPSGAAIDWEDGELGQTAIVRMRSAPFPH
                     ASRAAGFKSGDKVFPRDPHYTDNSVALFIPKGYRPEKQTDLLIYLHGHFNNIHRAMFE
                     CRLREQVVASGRNVILVFPEGPKESPDSGGGRLEEEGGLKHLVEEALDVLVAERKIAG
                     KELGRVALAGHSGAYRAIAFCLERGGLEDHVGPVCLLDASYGLLDNFVDWLAGNQDAR
                     LFSVFTAHLADENVYLMTHLRTLKVSYELLDEEDASEAIIQRARVAFVNTQKLNHNET
                     VRWLERWLRASPLPPRPGAVSSHPAE"
     gene            complement(9610..>10297)
                     /locus_tag="P5316_16635"
     CDS             complement(9610..>10297)
                     /locus_tag="P5316_16635"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86640.1"
                     /translation="QLWLGLVSGLLVAGMGLTLIWRACRGSLARHHHDHGHPELRSRF
                     RRLFSHSHVHPSPHAHDHPRDHATAHHHGHDHGHPHDHNHAAMPPMPYATSPSATASD
                     RVTGRMILMLGITGGIVPCPTATIIMLLGIGANVVVGALYAVAVFSLGLALTLMLIGF
                     LALSSRRFAARLMSSDDQAERLSGGGRLILLRVVPAVSGLAVVLLGLAISANYVHYMR
                     TGTTLFRWMG"
BASE COUNT         2054 a         3171 c         3220 g         1852 t
ORIGIN      
        1 agccttgggc ggcgggacag gacaggcgga cggttgcgcg accggccgac ggtgtcgggc
       61 ctggacgatc atcgaaacca gtagcacaat acccaggccg gcggccacat atttgtcctt
      121 actcattacc gagttcaggg tctccggcga gtaggttgcc agcagcatgc cgaggcccag
      181 cgagatcatg gccacgccga tgcaggcggt tcccagaatg gttgtgaacc gcggcaatgc
      241 caagcccatc ccaaggcctg tgatccaggc cagcaccgcc acgatgacca ccttggccac
      301 gacgttttgc cgcgtctgac ggaaatagga aaacgcatcc acgacatatg ctttcagggc
      361 ccatgcgacc tggcctatca tgagtgttgg actgggtcgg ctttcgggcc cggctgatgt
      421 ttccggcggg ggtggtggca caatcagtac cttctgctgc tggtgatact ccgtcagggc
      481 cgagataagg ttctctgcgt tctggtttgc cgccacggtt aacaccagca gggccgcgca
      541 gcaggcggca gtcacgccca cccaccagcg aaacagcaga tgcccgatca ggccgatgag
      601 accggcacca atgaccaagc cgatcagcag gtcaacattt gtttttctgg ccacggtcac
      661 cccgaggaag ccgccggcaa caatgaacgc caggacgtag atcagccgaa gcagacggct
      721 gccccagaag gccaagatga ctccgaggac gagcaggacg gcgatgaggg tcggtgggtt
      781 cggccaccag cttggcaggg acgcatcaac cccgcctttc gccgcccagt cggcgagaag
      841 gttgaagatt tctctgagac ccgtcattcg gcacacctcg gcccgcccct gcgacccggc
      901 ccgggccgct attgtttcga tgcccttggc ttcttgcggg ccagtcggct attgcttgcg
      961 gcggctggcc agggcgtcgg ccttcggcgt ggtatctggc gcacgttcga tgccggcatt
     1021 cgagtacacg ctcaggtcga tcgatgcctg cggagaaccg ggagcgaagc acttggccag
     1081 cattctcaac ttctcagagg ccgatggctc ctcgatttcc tcgccgattg ccgaacccca
     1141 atggaacgct tcactcttct ttgccctcgg atggatcaga atatggctct ttgccttgtt
     1201 cacccgaccc cggtggttgg tgacgtccac cgccgcgacc tggctgtgga aactctgctg
     1261 gaagatcagt gcggcgagct tgatccccgc cttggcatcg tcggcttcaa acggccggcc
     1321 cacttcgggc ggcttggctt tgacgcccag gatctggatc caggctccgc agccactgat
     1381 gtcgacatat gtctcgggca gacggtagcc atgcttgtca acgggcacgt agatcggccg
     1441 gccattgccc ggttgttccg tcaacagcat cgcgatgggg cgccggcaat cacaagtgat
     1501 tcggatggta ccatccggcg tgtacacgac ccgtcgcact tgcgacaccc agccgctatc
     1561 gagcaactga cgggcgatat tctggagcag cgattcgtcg gcgccgaggg gttcttgggg
     1621 cacgcggacc gatgagagaa tccgcgggcg ccagccctct ttatccaccc aatccatgtc
     1681 gggcggcacg tccagctcaa cgcgaataca ggtcttctgc cccggctcct gcgtgacgtg
     1741 acgctccagc cgctccagcc cggcgacgac cgagacgaga aaggccacaa ggaagacggc
     1801 cgagacgatc ggtcggaacg cccgcacatt cgcgcgtagg ctcgaccaag tcgactcatc
     1861 cgccgagttg ccgtcaccac ggcgctgact gctccggctc accactgtac tccccgacga
     1921 ccaccgagac tgcttaccgc aggtacgtga gttccacgat gcgctgacac aattgcgaga
     1981 aacccagacc cgcctgttga gccgccttcg gcagcaatga atgatccgta aagccaggga
     2041 tcgtgttgac ctccagcgcg aacggccggt tggtgctctc ctccacgatc caatccacgc
     2101 gcgagaaatc ccggcagccc aacgattgat gagcccggac gctcaagtcg cagatctcac
     2161 caagcaaggt tgccggcagg tcgatattga aaaggtattc ggtgtcgttg tccagatact
     2221 tcgcctcata attgtagaat tcgcgtttcg gccgaatctg gatcggcggc aagggttcgt
     2281 cgccgaggac gcccacggtc agctccgacc ctttgacgaa acgttcgatc atgcactggc
     2341 cgtagatctc gatctctttg cgaatcggcg cctcgaggtc ctggccttgg tgaacgatca
     2401 cgcagtccac gctgctgccc tcgcagaggg gtttgacgac ggacggcggc gtccactgcg
     2461 cgagcacctg ctcgatgttg tcgggcgtga ccacttcgaa caacggtgtg gggacgccca
     2521 cctccatgaa gcgggacttg gcgagggcct tgttcatcgc catggccgac gcctgcgggc
     2581 cggaaccaca gtacctgata cgtcgatcat cgaggatctt ctgtaactgg ccatcttcgc
     2641 cgaacgcccc gtggagggcg atgaagacca tatccaccgg tcggtcaagg gcggacaagt
     2701 cctcagggct gatgtcggcg agatagacgt catgtccgag cgatttcagg gccgccgcca
     2761 cggcccgccc gctcttgagg cttacttctc gttccgaacc aggcccaccg gacagaacgg
     2821 tgattcgcag gcgacgctcg accgtcttgc tgttgactgg tgtaaaggcg acgtgatctg
     2881 ctatttgtgt catgttggac ttccgtttcc tcttgaaacc atccttagcc aaggcggtcg
     2941 ctcattttag cggatgggct cgccggcgcg aagccgccgg cgaccccaga tctggacttc
     3001 cgtctcgaat tcgacgccga agcgatcggc cacctcccga cggatgatgc cgatgagtgt
     3061 cagcacatcc tgtgcggtgg ctccggcctc ggtgacgatg aaattcgcat gcctgggcga
     3121 aacgacagcc cctccgaccc ggcggccctt gaggccggcc tggtcgatga gtccgccggc
     3181 gcttgcaccg ggggggttct tgaacacaca gcccgccgag ttatctgcca gcggctgagt
     3241 gttctttttc atttcccaga tctcccggaa ccgagccttc aactgatcgg gatcggccgg
     3301 ctccaacgtc aagccggcgc tacagacgac ggtgccgccc aagtttgagc cgcgataccg
     3361 gaagccggcc tccttacggc tgagtgtgcg caattcgccg gccgcgtcga caaccgttac
     3421 gtggtgcacc acatcgcaga tgttaccaaa ccggcccccg gcgttcatgc ggaggatccc
     3481 gcctatggtg cccgggatgc cggccattct ctcaagtccg cccaagccgc tacggactgc
     3541 atcggaggtg aggcggtaca tatcggtgcc ggctgcgacc gaaatcgtaa ccggcttatc
     3601 tcgcttgcgg gcatcttcgt tgccccagtc tacggattga aagactggtt cgcccagacg
     3661 gatcaccaaa gcgtccacgc cttcgtcgct gaccaggaga ttggccccca gacccaaggg
     3721 aagaaactcg acaccttcgc tgttgcagcg gcgaaccagt tcccggacgt ggtcgatgga
     3781 tcgaggtctg gcgaaccaac gagcaggacc gccgacgcgc agacaagtgt gcgaggccag
     3841 cggctcgttc tgggtgacga tcccggaaag gtctgagaga aaatccgttt tcatgtccgt
     3901 gcccagcgcg gccccggccg cgcgtccatc tcaggtcgtc ccgtcccggt cgcggccgaa
     3961 ggctgcagtg cgccgaggcc ggggagctta atgatcggca atgtcgccca tggcgacaaa
     4021 cgggtcaccg tcttgctgcg cgccggccag cccggcgagc acgctgtcgg ccacctgcca
     4081 gacgttaccg gcccccatgg tgatcaccac atcacccgga cctacctctt cgcacagcat
     4141 tctggtgatg gagtcaaaat cggcctcgta gcgggcatca cggtcttgtt ggcggagcag
     4201 atccaccaag tccttggaat cgatcagttc tcgttccgat tcgctgtcgc gcacgaagta
     4261 gatgtccggc acgaccacca cgtcggcggc tccgaagctc ttggcgaagt cattgagcaa
     4321 gaaccgggtg cgggagtgct ggtgcggctg aaacacaaca tagagcttgt tcgggttgta
     4381 gtactcccgg accgtcttga gcgttgcctg gatctcgcgc ggatggtgag cgtagtcgtc
     4441 gagaaccgtt actcctccta tgctgcctcg cggcgtcatc cggcgatggg ccccgcggaa
     4501 gtcggccaga gcccgcgcga tgacctcagg ccgcaccccg cagtagaaac agacagcggt
     4561 cgccgcgaga gcgttcataa cgttgtgccg ccccggaacc ccaggcgtga tctcggtgag
     4621 cacctgccct tttcgaaaga ccctgaaacg atacgagccg ttgcggactt cgacgatctc
     4681 gccccgccag tcgaagaggt tctggcaacc gaaggtttcg accgtcgcct tggctccggc
     4741 taccgcctcc atggtgttcc ggtcttcgcc gttgaccacg agaagtccgt tcgccggaac
     4801 cagggccgcg aagtcgtgga atgaggagat aatttccctg atgtcgcggt agtagtcgag
     4861 atggtcctca tcgacgttga ggaggatggc gcaacgaggc cgcaggttat ggaatgatcg
     4921 gtcgtactcg caggcctcga cgacgaattg ttctccgtcg ccgacacctg atccgccgcc
     4981 gagttgctcg acacccgcgc cgatcacgaa agagggatcc agacctgcct cacgaagcac
     5041 gtacgcaatc attgcggtgg tcgtgctctt accgtgggtt ccgccgacgg cgatacccag
     5101 tttacgggac atgagcagcc cgagcagttg ggaatacttg acgaccttaa ggccgcggcg
     5161 gaccgcttcc atcatctccg ggtccgactc cttgacggcg gcggaatgca cgaccagatc
     5221 gcacggatcc ggaacatgac cgggcttgct gccgatgtgg atgttcgctc cctgttccga
     5281 gagcaggacg agggcggttg actccttgcg gtcggagccg ctgaccctgg cgcctgcgcg
     5341 catgagcacc cgggcggctc ctcgcattcc gcatccgcca attccgatca tgtggacccg
     5401 caggcccacg gggttgaaaa caccgtcctc ggcggccggt cggtccggct tcgtcaaatg
     5461 cgtcggcgga ccgtcaagag ccaaaccgtt gctcgcctca caattggccc ctgctacatg
     5521 ggacatgaaa gacccttccg tgtgatgtga aacatctcca tcaggcgacc gaccgcacct
     5581 ttcccccggg cttgcccatc acaccttcac aagccgacag ttgccccctc ccgatcaggc
     5641 cggctccaca acctgacaga ctgagaaatc caggagccag ggcattgttc tcgctcaagg
     5701 cgcttcggct tggtgtgcac attctactgt gaacgaggca ttggtccaaa cacgccgtcg
     5761 accgccggcg gtgcacctcc ggcgcaagcg tgcggcccgc actaccaagc tgaaccgctt
     5821 cactagttgt atcgggagtc gcttcagctt tccttgagag acggtaagga gttgcgttgc
     5881 cgaagagaaa cgtcgcatgt ccggctgctc cagccgagga ggtccgctaa aaatcaatgc
     5941 cggcagactg gcagagatgt tgtgcaatct ttgcggccgc ttggggcctg cccaacccac
     6001 aggctgccgc gcccatgcgt tcgcgatgcg aggcgttgtt gaggatatcg gtcaagaccg
     6061 gccggatctg gcgagcgttt tcctcgccat ccttgaggtc gtcaagaaga atagccgctc
     6121 cggctttaac caggacttcg gcgttatgcc actggtggcg gtcgcgatga aagggatagg
     6181 gaagaaggat ggatggtttc cctaccgcga gaatttcagc caatgttgag gcgccggccc
     6241 gcgagacaat gaggtcggag gcgagcatgg catgagccat tcggtctgtg aagggcagga
     6301 cggcggctga gaggctggac cggcggtatt ccctagcgac gcgttcgtaa tctgcctgcc
     6361 cggtgaggtg gagcacctgc cagccggccg aagcgatttg atcagccagc cgaatgaacg
     6421 cctcgttgat ggtgcgtgct ccctgggacg ctccggtgat caggagcatg gggcgagacg
     6481 gatccaagct gaaagattgc ttcaactcgc ccattttatc gcggacggct tcaagcgtat
     6541 tgagcttggc ggcagactcg cagacggacc tgaaagcttt tcgtactggg cagccggtga
     6601 cgtagactgg cgctgtcggg ggaaaagagg ctttggtcac gtcccactga gcgaacacgc
     6661 cagcgagatc ggggcgcttg ccgaggtgtc ggttggcccg tccgggtacc gcgtcaggat
     6721 tcagcaggaa cgtcgggatg cccatcttca acccgacatg gacaggcggt ccggaggcat
     6781 atccgccagc tccgacaatc accgctggtc gtcgctgctg gaaggcctgc cggcagacag
     6841 agacggactg gcgccaatgc cacagaaatc ttggccatgc ccatggacgg gtcggaaacg
     6901 gcagtacgga caaagggtag gcctggatct gagcttcgtc gagcacattc tggtcgatgg
     6961 ggcgttgcgt gcagaaaaac gagacgtcaa ccggcaggcc gcgttcacga agcaattcca
     7021 cgacagccaa cgcggggaaa aggtggccgc cagtgccacc gccggcgaag acgaaccaat
     7081 ggcgagactc gctcacattc gtgcctttcg catgaaagcc catcgatgag ctaccgagag
     7141 aatggccatg ccttcggatt gatcgcccgc agctcgggaa tcaggccttc tccggtggtg
     7201 ccgaaccgga gtgccgtgca atgctggcca gcaccccaac cacacccccg aggaaaaccg
     7261 taccggatcc accggcggag accagcggca gggcaatgcc cttggtagga accgaggcgg
     7321 tgacgacggc gatgttcatg gctgcctgga tgccgaacat cagcacgatg ccgaaggcaa
     7381 ggaggcgacc gacggggtca gggcatcgaa agacgacgct tcgccccagc cacaagaaag
     7441 tgagcagcag cccgatcacc gcgattccac cgacgatccc caactcttcg cagatcaccg
     7501 cgaagatgaa atcgttacgg cttccggaag gtatcctttc acgaagccgt tgcccaagcc
     7561 gcgcccccac cagccgccgc tgataatggt gcagagggac tggatgatct ggtagccttt
     7621 tccggtcgca tcctggaaag ggtccaggaa gatggtcaag cgatcgacgc ggtgcgactt
     7681 gctgatgatc agatagccaa aggccggtac tgcgggcagg gtcagcaaca gcaagtgcca
     7741 gatcttcgcg ccggcgacaa ggagcattcc gccggcgaca agggccagca aggcggcggt
     7801 gccgaagtcc tcgatgccga tggtgccggc gcagagggcg ataacggcgc ccatgggcac
     7861 aagcccggtc cagaatttgc ggatatcaac cctatagacg gtccaggtgg ctatgaagat
     7921 gggcaaggct cccttgacca gttccgaggg ctgaaaccgg ctcgtggtgc cgggtatttt
     7981 caaccaccga taggcaccgt gggaggccac gcccagcccg ggcacgaaca cgagagaagc
     8041 cacgccaagg gcaaggatga gcagaatcaa cgccagataa ccgcgcccga gggagaacca
     8101 ggtgtaaggc acccgtgtca tcagcagcat ggcaagcaag ccggcggtga cgaaggccaa
     8161 ctggcgtatg gcatcggtct cccagacggg ccagatgacc agcgggtggt cgattgaggt
     8221 actggccgag aaggtcatga cggcgccgat ggccatgagt ccaccagcca ccgcgacaat
     8281 ccagacggag gtagcgtggg ctctttcctc ggcagtcaga ctctctgtct cggacaacgc
     8341 gggatccatc tccatcgtca gagcatcgct catggtaatt cagctttcga gccggtcgac
     8401 ccggatgatc ccattcgtga ggagcggccg ggccgccatc gtcgtcgatc agactgccta
     8461 ccggaccgcg tggttccgca cggccagacg cccctctccg caggacaggg tttcaccccc
     8521 tctccctggg aagaccagag agtcgatcct gtaatcatcg gcggacagcc gcctcggcgt
     8581 ttaacattcc tatccgcgaa acaactgagg gggccggatg gccgtggccg ttgttcgatg
     8641 ccggtcctgc ccgacgcgca atcaagcgac tttcgggccg cggcggtaga atgatggcac
     8701 gttgaattcg tggaggccaa catgagtaca ctggcattgc tcggcatcct gtgtgtcgca
     8761 agctatgagc cctccggagc ggcgatcgac tgggaggacg gcgaacttgg gcagacggcg
     8821 attgtccgga tgcgcagcgc cccctttcct cacgcgagcc gtgccgccgg attcaaaagc
     8881 ggcgataagg tctttcctcg cgatccgcac tacacggaca acagcgtcgc gctgttcatc
     8941 cccaagggct atcgccctga aaagcagacc gacctgctga tctacctcca cggccatttc
     9001 aacaacatcc accgggcgat gtttgaatgc aggctccgcg agcaggtggt cgcgagcggc
     9061 cgcaacgtga tcctggtttt tccggaaggg ccgaaggaat cgcccgactc cggcggtggc
     9121 cgcctggaag aagaaggcgg cctcaagcac ctggtcgagg aagcactgga cgtcctcgtg
     9181 gccgagcgga agattgccgg aaaggaattg ggacgcgtcg cactggccgg ccatagcggg
     9241 gcctatcggg cgattgcctt ctgcctggaa agaggcgggc tggaagacca cgtcgggccg
     9301 gtgtgcctgc tggatgcaag ctacggcttg ctggacaact tcgtggattg gctcgccggg
     9361 aaccaggacg ccaggctttt cagtgttttc actgcccact tggctgacga aaacgtgtac
     9421 ctgatgacgc acctgcgcac gctcaaagtg tcatatgaat tgctggacga ggaggatgcc
     9481 tcagaggcga tcattcaaag agcgcgtgtc gcttttgtca acacgcagaa gctgaatcac
     9541 aatgaaacgg tgcggtggct cgagcgctgg ttgcgtgcga gtccgttacc gccgaggccg
     9601 ggggcggttt catcccatcc agcggaatag ggtcgtgccg gtgcgcatat agtggacgta
     9661 gttggccgaa atggccaagc cgagcaggac aacggcaagg ccgctcacgg ccggcacgac
     9721 gcggagcaga atgagcctgc cgcctccgga gagacgttcc gcctggtcgt cgctggacat
     9781 cagccgagct gcaaaacgtc ggctggacag cgccaggaag ccgatcagca tcagcgtcag
     9841 ggccaatcca aggctgaaca cggccacggc gtagagcgct ccgaccacca cgttcgcgcc
     9901 gatcccgagc aacatgatga tcgtagccgt cgggcaaggg acaattccgc cggtgattcc
     9961 caacatgagg atcatgcgtc cggtgacgcg atcggaagca gtggcagacg gcgacgttgc
    10021 atacggcatg gggggcatgg ccgcgtggtt gtggtcgtgc ggatgcccgt ggtcatgacc
    10081 atgatggtga gcggttgcat ggtcgcgggg atgatcgtgt gcatgggggg aaggatggac
    10141 atgtgagtga ctgaacaggc ggcggaaccg ggatcggagc tcgggatgtc cgtgatcgtg
    10201 atgatgccgc gccaggctgc cgcggcaggc ccgccagatg agggtgagcc ccataccggc
    10261 gacaagcaac cccgagacaa gcccgagcca gagctgg
//
LOCUS       DAOTPW010000209        10198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6154_length_10198_cov_5.210589, whole genome shotgun sequence.
ACCESSION   DAOTPW010000209 DAOTPW010000000
VERSION     DAOTPW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6154_length_10198_cov_5.210589"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1682
                     /locus_tag="P5316_16640"
     CDS             210..1682
                     /locus_tag="P5316_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRR86641.1"
                     /translation="MVSELPEGESPERLTDAQSPRLSADPREALLNKIRHLPRTPGVY
                     LFKDAQGRVLYVGKAKDLRSRVSSYFQNSTDLLATRGPEIAHMASLAVDLDFLDCETE
                     VDALLQENRLIKDIQPPYNDRLRDDKTFPYLEITTSDDFPGVYVTRKPRLKGNKLFGP
                     FTNAAGVRDAVNALQKVFKFRTCELEIVDGDERRRYFRPCLLHSISRCTAPCADRISK
                     EEYRKDIERLKKFLASKRSVVLRQLNREMEQAARHQEYEIAARLRDEIKALEALSLSG
                     DPNADVQPEVFYIDPAEGLAKLGEILDLDRAPRSIEGIDIANLHGEESCGSLVCFIDG
                     KPFKNGYKRFRIKTVEGIDDYAMIREVVLRRYRHAAEGEELYPDVILIDGGLGQLHAA
                     LSAFDEMNIAPPMVVSLAKRDEEIYIQARSRPVRLARNDPALRLLQQVRDEAHRFAQH
                     YHHLLRRKKTFEEDVAAGRKPPRAARPEGGRPKRPGASGQ"
     gene            1943..4687
                     /locus_tag="P5316_16645"
     CDS             1943..4687
                     /locus_tag="P5316_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRR86642.1"
                     /translation="MFVISPAWGRSTGADKHLPIRLATVMVAASLIFCISEASAACGF
                     DWKPGESPQGLSYEGYAMAVYDDGGGPALYVAGGFETAGGVKVCGVARWDGRYWSPVG
                     GGVSKPSGCGQVKALVVHDGELVAAGSFAMAGNTAAANIARWNGSRWAPLGEGTNFDV
                     LALAIYGNDLIAAGNFTTAGGVSANRIARWNGETWSPLGSGLNDAVGTLHVHEGELIA
                     GGRFTMAGGLKANQIARWNGSEWQSVGGGVTAGSNPNVSALLSYGDHLVVGGFFAALG
                     GVACKNIGIWDGSKWAPLGEGTDASVLDLTLYNGELIAGGFFEKAGGKPARHIARWDG
                     ETWSPLAGGLMSYVIALAVYGDDLIATGSGTVAGGVRADNIARWDGTRWFPLGAAMDH
                     SPYAFADYNGELIAGGEFTYLNGVRCNGIARWSGSAWVSLGEGVAGGKDGTQVRALTV
                     YRGELIAGGNFAQAGRVGAKNVARWNGSSWAPLGQGFNGPVRALIVCKGELIAGGTFT
                     TSGGTTLNGIARWDGEAWMPLGNGVSGTWSPGVYCLAAFAGDLYAGGLFLKAGGETVN
                     HVARWDGAEWHSLTGGVTGSGYPDVPVTALAVFNDELIAGGLFTAAGGVHAAGVARWD
                     GKGWSAMEDENAGGPRQVHSLAVHNGQLIVGGSFPSQGLLSADGLGRWDGAAWSPVGR
                     GIAMGSVYAMAVHRGELLAGGSFLAAGGKTSARFARWAPVEPTVEIHQWRSSRYHGGS
                     ISGELAVRIDASATTGRRITTETRQGGVQKIIVDITGPEDGRLELEGRVAAEEVGGAG
                     YSYPAVHQTLTHDGEGSYTLVLEWPEGLPDQQSYRIDLGANLTCLKGDTDALIRSLTG
                     DVTGDGVVDSADLLEAKSRISRPVMPDNIIFDVNIDGSLNATDVLTIRAFQDRAAMSP
                     "
     gene            4979..5818
                     /locus_tag="P5316_16650"
     CDS             4979..5818
                     /locus_tag="P5316_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR86643.1"
                     /translation="MMTLAVACVSAFFLPEAGASDASNRFVIISDTQNIEGVDQMTRE
                     IIELAPSFAVAVGDMPSGFDTRVSFFRRLREANIPIHIAMGNHDGGTKMAVRSCLPPY
                     PFNDEVDPALRFVVENKYYYSFNRGGIHFVIVDTSTADTEQHIQWLEADLIRHVNNPK
                     KYPTLLFMHYPDWMMGKNAGTGGPVYRVLAAHPEEHTVKAAFAGHTHDGKHYPPEETL
                     GIPLYTLYPSAPFGDATHTEYVVATVTPNAIAFERKAILDEGRGKDFVIQPVKGEFGR
                     LDK"
     gene            5815..7800
                     /locus_tag="P5316_16655"
     CDS             5815..7800
                     /locus_tag="P5316_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86644.1"
                     /translation="MRQSHLQSSILLFLLGVPVAAAAVDDSGIMVHGSTGAPGGTAAG
                     LTTYLSDPKMGPPIICRPTHEYSGQWVARVPGRGVYTAAIEEAYGFGPALRPAAIVSE
                     TGFNRIHIELPFQYDLLQGGKPADADHAEFGQVFVAPGTAITGIAFQNADGVLIALHE
                     GNAAGRQIGQAVCASSNYPYGALPTIPGGKYYLRFTRADGSPFRMHVVADNRYPDGSA
                     YMDGKSQSSLDLALRIQYNPAGQIIRHRPRFTEVYKTASKTYGQTFVAKGTSLAMLSV
                     FPAVGDGPTVAPIIRILESGPEGKALGPAIESRILLFSPGQMNLAPGKRYYIEVSAPA
                     KDGPVRLWTSRTDDFKDGELFVDGTPVADRDLAVILIEYEPDKVPPPSATAVNWRPGA
                     SPYAQRYSADGRVRLVWDIPADGDITSMIISRLRLSEGGVQAEPELIAEIPASAPGRY
                     QYAESGLENGQPYRYTIRTVDAAGNESEPLAGPVMPRPAIPMVAELLNGDFSGPGDSG
                     LPYGWMTKTIAGTVPAFKLDAPDGSRNPIPAAGWEVSEEARFSDTVLYQHVPCEKGRR
                     YQLAAEVRLWNPWNNQQMIICAQLGIDPTGGDDPLGRSVVWGSPTYQREKWTMLATAA
                     AAEADSITVYLRGHSQYSHLMNTRFRSVTLAEIPVGR"
     gene            7855..9606
                     /locus_tag="P5316_16660"
     CDS             7855..9606
                     /locus_tag="P5316_16660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR86645.1"
                     /translation="MTQGLGMRVLFIYPNLNAEEGFNHGVAALSGGIKLRGHVTGLLN
                     INEALYEIPGDEEIVEKIRQWRPDFVAFSVMTQQYKFALRLAKAIRRAMPAVPIGVGG
                     VHAIMCTQQIVNDGFWDYIGVGECDTAFPELIDKLAAGDPTARDTPNFLVRNADGTYR
                     ENPLGPFPDPADIAPTDYELYDLPHMLPRKNGWQSMLTSRGCPYRCSYCFNHEVSDRY
                     FTEGGYSRRSYLRNYPIDRIIGEMRELKSRHPYLETFIFDDDLFTLNKDYCIRFVEAY
                     TASGINVPFVLNAHVQAFSEPIAKALSQSPCMIVKFGVESGSDELRRKILDRHMSNQA
                     IIDAFDLCHRYGLHSSAFLMFGLPYETRSMMEETIDLMAKIRPGRFRWAIFFPFPGTK
                     SYTICQLGRLIDYRKMDAMDNYFCASCLKFDEAADLFIRKLQRTFHWYVNARAGLPLS
                     SEYQKMVDDVEAMDAAAWHEASETLLARDRKISNDHLAKVKPGDAEAMDRLRHYSIRY
                     TEVMAVSSDFVLAEKGDYKNEAARRWKAFREQIDQARAAAAEMATAGAAARPSPKDAP
                     LPMLRITESPGPYSNGS"
     gene            complement(9987..>10198)
                     /locus_tag="P5316_16665"
     CDS             complement(9987..>10198)
                     /locus_tag="P5316_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86646.1"
                     /translation="FEHRARAGRQPVVPGERKGRRGRRPHLFSICVNPMICGPDGYDQ
                     DQPLGTAPSTLLPSGLPSAMSSLRA"
BASE COUNT         1939 a         3081 c         3263 g         1915 t
ORIGIN      
        1 aattggccga ccgtgaacca gtcgtaaaac gaccggcctg tttgcccttc gggaaagacc
       61 gctgaagctg aagcggtctg cttccggctt gcgttcacga caaccgggtc agccgccagg
      121 agtgccgccg cttctgttac cggccccgta gccgctcgcg ggagcagacg tctcatcgaa
      181 tggaggccgt gatgatataa tatcacgcca tggtttccga attgccggaa ggagagtcac
      241 ccgagaggct gacggacgcg cagtcaccga ggctgtcggc cgatccgcgc gaggccttgt
      301 tgaacaagat tcgtcacctg cccaggacgc ccggggtgta tctgttcaag gacgcacagg
      361 gccgggtgct ctatgttgga aaggccaagg acctccgcag ccgcgtgagc agctatttcc
      421 agaactcgac cgacctgctg gccacgcgag ggccggagat cgcccacatg gcctcgctgg
      481 cggtggacct ggactttctc gattgcgaga cggaggtcga cgccctgttg caggagaacc
      541 ggctgatcaa ggacatccag ccgccgtata acgaccgact tcgtgacgac aagaccttcc
      601 catacctgga gatcacgacc tccgacgact ttccgggcgt ctacgttacc cggaagcccc
      661 gactcaaggg caacaagttg ttcggtccgt tcaccaacgc cgccggcgtt cgcgatgcag
      721 ttaatgccct tcaaaaggtg ttcaagttca gaacgtgcga actggagatc gttgacggcg
      781 atgagcggcg gcgctatttt cgaccgtgcc tgctgcattc catcagccgt tgcaccgccc
      841 cctgcgccga tcgcatctcg aaggaggagt accgcaagga tattgagcgg ctcaagaagt
      901 ttctcgcgtc caagcgaagc gtggtgttgc ggcagttgaa tcgggagatg gagcaggccg
      961 cccgacatca ggaatacgaa atcgccgcca ggctgcgcga cgagatcaag gctctcgaag
     1021 ccctctcgtt atcgggagat ccgaatgcgg acgtgcagcc ggaggtgttc tacatcgatc
     1081 cggccgaggg attggccaag ctgggcgaga tcctggatct cgaccgggct ccgcggtcta
     1141 tcgagggaat cgatatcgcc aatcttcacg gcgaggagtc ctgtggttcg ctggtctgtt
     1201 tcatcgacgg taagcccttc aagaacggct acaaacgttt ccgcatcaag acggtcgaag
     1261 gcatcgacga ctacgccatg atccgcgagg tggtcctgag gcggtaccgg cacgcggccg
     1321 aaggagagga actatacccg gacgtcatcc tgatcgacgg gggtttgggg cagctccacg
     1381 ccgctctgag cgccttcgac gagatgaaca tcgccccgcc catggtggtt tctctggcca
     1441 agcgggacga ggaaatctac atccaggccc ggagcagacc ggtccgcctg gcacgtaacg
     1501 atccggcgct gcgtctctta cagcaagttc gcgacgaggc ccaccgcttc gcgcagcatt
     1561 accatcacct cctgcggcgc aagaagacct tcgaggagga tgtggccgcc ggacgcaagc
     1621 cgcccagggc cgctcggccc gagggggggc gtccgaaaag accgggcgcc tccgggcaat
     1681 aggcccggat tacctgtcgc ccttggcggt cgcctggaac gtccggaagt gcatggcatg
     1741 ggggtcgtcg gagactttct cccctgcggg tcagaatgaa caattcttcg tccgacccgt
     1801 ctcatactag tggttacctt gcgtttgggc gtcttgcatc gtaggattca ctggaaggcg
     1861 gcgtcaactg agcgtgtccg gcgtttgact ggcataaagg gggacgccgg tggctgcgcc
     1921 cactaatgga gaggcctgaa agatgtttgt catttcgccg gcatggggtc ggtcgacagg
     1981 ggccgataag cacctgccga tcaggctggc aacggtgatg gtcgcggcgt cgctgatctt
     2041 ctgcatatca gaagcctccg ctgcttgtgg atttgactgg aaaccagggg agtccccaca
     2101 gggtctgagc tacgaaggct atgccatggc cgtttacgac gacggcggcg gcccggcact
     2161 ctacgttgcg ggggggttcg aaacggccgg cggcgtcaag gtttgcggcg ttgctcggtg
     2221 ggacggacgt tactggtcgc ccgtcggggg tggtgtgagc aagccctccg ggtgcgggca
     2281 agtcaaggcc ttggtggtgc acgacggcga actcgtggcc gccggttcgt ttgccatggc
     2341 cggcaacaca gcagcggcca atatcgcccg ctggaacggg agtcgctggg ctccgctcgg
     2401 cgagggaacg aatttcgacg tcctggcgct ggcgatttac ggcaatgatt tgattgccgc
     2461 gggaaacttc accacggcag ggggcgtttc tgccaaccgg attgcgcggt ggaacggcga
     2521 aacatggtcg cccctcggtt cggggcttaa tgacgccgtc ggaacgttgc acgtccacga
     2581 aggggagctg atcgcgggcg ggcgattcac aatggccggc ggattgaaag ccaaccagat
     2641 cgcccgatgg aacgggagtg aatggcagtc cgtgggaggc ggagtgacgg ccgggtcaaa
     2701 ccccaatgtc agtgccttgc tcagttacgg ggaccatctg gttgtcggcg ggttcttcgc
     2761 cgcgcttggc ggcgttgcct gcaagaacat cggcatctgg gacggaagca agtgggcacc
     2821 gctcggcgag ggaacggatg cgtcggtcct ggatctgacg ctgtataacg gggagctgat
     2881 tgccggcggg ttcttcgaaa aggccggcgg gaaaccggct cgccacattg cccggtggga
     2941 cggcgagacg tggagtccat tggcgggcgg gctgatgagt tacgtcattg cccttgcggt
     3001 gtatggagac gatctgatcg caaccggctc gggaacggtc gcgggaggtg ttcgggctga
     3061 caacatcgct cgatgggatg gaaccaggtg gtttccgctc ggggcggcca tggaccacag
     3121 cccctacgca tttgcggact acaatggtga actcatagcg ggcggagagt ttacgtacct
     3181 caatggggtc aggtgcaacg ggatcgcccg gtggagcggg tcggcgtggg tctcgctcgg
     3241 cgaaggcgtt gccgggggca aagacggaac tcaggtcagg gctttgaccg tctaccgggg
     3301 tgagctgatc gccggcggca atttcgctca agccggcaga gtcggtgcca agaacgtggc
     3361 gcgctggaac ggcagctcat gggcgccgct cggacagggc ttcaatgggc cggttcgtgc
     3421 ccttattgtc tgcaagggtg aactgattgc cggaggcacg ttcaccactt cgggggggac
     3481 cacgcttaac ggcatcgccc gctgggacgg ggaagcatgg atgccactgg gcaatggtgt
     3541 aagcgggact tggagtcccg gtgtctactg tctggccgcg ttcgccggcg acctttatgc
     3601 gggtggcttg ttcttgaaag ccggcggcga gacggtgaat catgttgccc ggtgggacgg
     3661 ggccgaatgg cattcgctta ccgggggagt caccggctcg gggtatcccg atgttccggt
     3721 cacggcgttg gccgtcttca acgacgagct gattgccggc ggactgttca ccgctgcagg
     3781 gggcgttcac gccgccggtg tcgcgagatg ggatggcaag ggttggtccg ccatggagga
     3841 cgagaatgct ggcgggccgc gtcaggtgca cagccttgcg gtccataacg gccaactgat
     3901 agtcggtggc tcgttcccca gccagggtct gctttcggcg gacggcctgg gccgctggga
     3961 cggcgcagca tggagtccgg tcggtcgtgg aattgcgatg ggctcggtgt atgcgatggc
     4021 agtgcacagg ggcgagttgc tcgccggcgg ctccttcctg gcggccgggg gcaaaacttc
     4081 cgctcgattt gcccggtggg cgccggtcga gccgacggtc gagatccacc agtggcgcag
     4141 cagccgctac cacggcgggt caatcagcgg ggagctggca gtcaggatcg acgcctcggc
     4201 caccaccggc cggcggatca ccacggaaac gcgacagggc ggcgtacaga agatcatcgt
     4261 cgacatcacc ggcccggagg atggcagact ggagcttgag ggccgggtgg cggcagaaga
     4321 ggtcggcggg gccggatact cctaccccgc cgtacaccag acgctgaccc acgatggcga
     4381 gggcagctac acgttggtac tcgaatggcc cgagggactg cccgaccagc agtcttaccg
     4441 catcgatctg ggagcgaacc ttacctgtct caagggcgat acggacgccc tcattcgcag
     4501 cctgaccggc gacgtgaccg gtgacggcgt cgttgatagc gccgacctgc tggaggccaa
     4561 gtcgcgcatc tcgcggccgg tcatgcccga caacatcatc tttgacgtca acatcgacgg
     4621 ctcgctcaac gccacggacg tgctgaccat ccgcgcgttc caggatcgcg cggccatgtc
     4681 gccgtgagac cggcgcaagt cttgccgtga gcgcggcacc cagagcagat gtgattccac
     4741 caccggcggc gtcctgttgt catcaagccc acacgaagga aagctgagac tcactgaata
     4801 tggccgacag gaggtcgtgt ccggttgcgg gcataatccg aggcgctata caatgaccac
     4861 gcgctcgcgg tgactcagtc gcgccattcg ggccgacgcg caatcacgca catccttgag
     4921 ggaatggaga accatgagac ttcgcagccg tacaccaaac ctcacaacat tatcagcaat
     4981 gatgaccctt gccgtcgcat gtgtgtcggc tttctttctt cccgaagccg gggcaagcga
     5041 cgcctccaat cggtttgtga tcatctcgga cacgcaaaac atcgagggtg tggaccaaat
     5101 gacccgcgaa atcattgagc tcgccccgtc gtttgcggtg gccgtgggcg acatgccaag
     5161 cggctttgac acccgggtga gtttcttccg gcggctgcgc gaggccaaca ttcccattca
     5221 catcgcaatg ggtaatcatg acggtggcac caagatggcg gtgcgatcct gccttccgcc
     5281 gtatcccttt aacgacgagg tcgacccggc cctgcgcttt gtcgtcgaga acaagtacta
     5341 ctactcattt aaccgcggag gaattcactt tgtgattgtg gacacctcca ccgcggacac
     5401 ggagcagcac atccagtggc tggaggcgga cctgatccgc cacgtcaaca acccgaagaa
     5461 gtatcccacc ttgctattca tgcactatcc tgactggatg atgggcaaga atgccggaac
     5521 gggcggccct gtttatcgtg ttctggcagc gcatcccgag gagcacactg tcaaggctgc
     5581 gttcgcgggg catacgcacg atggaaaaca ctatcctccc gaagaaaccc ttgggattcc
     5641 gctgtatact ctctatccgt cggccccgtt cggcgatgcc acgcacaccg agtatgtcgt
     5701 ggccacggtc acgccgaacg ccattgcctt cgagcgcaaa gccatcctcg acgaaggtcg
     5761 cggcaaggac ttcgtgatcc agccggtcaa gggcgaattt gggaggctgg acaaatgagg
     5821 caatcccacc tgcaatcgtc gattttgctc ttcctgttag gcgttccggt agccgcggcc
     5881 gcggttgacg attccggaat catggtccac ggaagtacgg gagcgcccgg cggtacggcg
     5941 gccggcctca caacttactt gtccgatccc aagatggggc ctccgatcat ctgtcgcccg
     6001 acccacgagt acagcggtca gtgggttgct cgcgtacccg gccgtggcgt ctataccgcg
     6061 gcgatcgagg aggcctacgg gttcggtccg gccttgcgac cggcagcgat tgtcagtgag
     6121 accggtttca atcgcatcca catcgagttg ccgtttcagt atgaccttct ccaaggcggc
     6181 aaaccggcgg atgccgacca tgccgagttc ggccaggttt tcgtcgctcc cggcaccgcg
     6241 atcaccggta ttgcgtttca gaacgccgac ggcgttttga tcgcgctgca tgaaggcaat
     6301 gccgccggaa gacagatcgg ccaggccgtc tgcgcgagca gcaactaccc ttacggcgcc
     6361 ctgcccacca ttccgggagg gaagtactac ctgcgtttca cacgtgctga cggctcacca
     6421 tttcgaatgc acgtggttgc ggacaaccgc tatcccgacg gttcggccta tatggacggc
     6481 aagagccagt cctcgctgga cctggccttg cggattcaat acaacccggc cggacagatc
     6541 atccgccacc ggccgcggtt caccgaggtc tacaagacgg cgagcaagac ctacggccag
     6601 acgttcgtcg cgaaaggcac cagtctggct atgttgagtg tttttcccgc tgtcggcgac
     6661 ggacccaccg tcgcaccgat cattcgcatt cttgaatccg gtcctgaagg caaggcgctc
     6721 gggccggcga tagagagtcg tatccttctt ttcagtccag gtcagatgaa cctggcacct
     6781 gggaagaggt attacattga agtgtctgcc ccggcgaaag acggcccggt tcgcttgtgg
     6841 acctccagga ccgatgattt caaggacggc gagcttttcg tcgacggcac gccggttgct
     6901 gaccgcgact tggcggtgat tctcatcgag tatgagccgg acaaggtgcc gccgccctca
     6961 gcgacggcgg tgaattggcg accgggggcg tcgccttatg ctcagcgata ttccgccgac
     7021 ggacgcgtgc gtctggtctg ggatataccg gccgacgggg acatcaccag catgatcatt
     7081 agtcgccttc gcttgagcga aggtggcgtg caagccgaac cggaactgat tgccgagatc
     7141 cctgcatctg cgccggggcg atatcagtat gcggaatcag gactcgagaa tggtcagccc
     7201 taccgttaca ccatccgaac cgtcgatgcg gccggaaacg agtcggaacc gcttgcaggc
     7261 cctgtgatgc cccgaccggc gatcccgatg gtcgcggaat tgctcaacgg cgatttcagc
     7321 gggccgggtg attcgggtct gccttacgga tggatgacga agaccattgc ggggacggtc
     7381 ccggcattta agcttgatgc tccggacggc agtcgcaatc caatccctgc ggccgggtgg
     7441 gaggtctcgg aggaagcccg tttttctgat acggtgctat accagcacgt gccttgcgag
     7501 aaggggcgcc gctatcaact cgcggccgag gtccgcttgt ggaatccgtg gaataaccag
     7561 cagatgatca tttgcgccca actcgggatc gatccgacgg gcggcgatga tcctcttggc
     7621 cggtccgttg tctggggctc gcccacctat cagcgcgaga aatggacgat gcttgcgact
     7681 gccgccgccg ccgaggcgga ttccatcacg gtttacctgc ggggccactc gcagtactcc
     7741 catttgatga acacgcggtt ccggagcgtc acgttggccg aaatccctgt cggccgttga
     7801 cgaccgatca gacgcctact agacttccaa cgtctgtcgt ggcacgagct tacggtgacc
     7861 caggggctgg gaatgcgggt tctcttcatc tatccgaacc tcaacgccga ggaggggttt
     7921 aaccacgggg tggctgcgtt gtcagggggg ataaaactgc gggggcatgt caccggactg
     7981 ctgaacatca atgaagcctt gtacgaaatc cccggcgacg aggagattgt tgagaagatc
     8041 cggcagtggc ggccggactt cgtggccttc tcggtcatga cccagcagta caagttcgcc
     8101 ttgcgtctgg ccaaggccat caggcgggcc atgccggccg tgccgatcgg tgtcgggggt
     8161 gtacacgcca tcatgtgtac ccagcagatc gtcaatgacg gtttctggga ctatatcggg
     8221 gtcggcgagt gcgacacggc ctttcccgag ttgatcgaca agcttgcggc cggcgacccg
     8281 accgcccgcg atactcccaa cttccttgtc cgtaacgccg acggcaccta ccgcgagaac
     8341 ccgctcggcc cattccccga cccggccgac atcgccccca ccgactacga gctttatgac
     8401 ctgccgcaca tgctgccgcg caagaacggg tggcagagca tgctgacctc gcggggctgc
     8461 ccctaccgct gcagttactg tttcaaccac gaggtatccg accgttactt caccgagggc
     8521 ggttattctc ggcgcagcta tcttcgcaac tatccgatcg atcggattat cggtgagatg
     8581 cgggaactca agagccggca cccctacctg gagacgttca tcttcgatga cgacctgttc
     8641 acgctgaaca aggattattg cattcgtttc gtcgaggcgt atacggcatc gggcatcaat
     8701 gtcccgtttg tgctcaacgc ccacgtgcag gctttcagcg agccgattgc caaggccctg
     8761 tcgcagtcgc cctgcatgat cgtcaaattc ggcgtcgaaa gcggcagcga cgagcttcgc
     8821 cgcaagatcc tcgaccgcca catgagcaac caggcgatca tcgacgcctt cgacctgtgc
     8881 caccggtacg ggctgcacag ctccgcgttt ctgatgttcg gcctgcctta cgagacgcgg
     8941 tcaatgatgg aagagaccat cgacctgatg gccaagatca ggccggggcg gttccgctgg
     9001 gcgatcttct ttccgttccc cggcaccaag agctacacca tctgccagct cggccgcctg
     9061 atcgactacc gcaagatgga cgcgatggac aactacttct gcgcatcgtg cctcaagttc
     9121 gacgaagccg ccgacctgtt cattcgcaag ctccagcgaa cgtttcactg gtacgtcaac
     9181 gcccgggcgg gcctgccgct gtcgagcgaa tatcagaaga tggtggacga cgtcgaggcg
     9241 atggacgccg ccgcctggca cgaggcctca gagacgcttc tggcccgcga tcgcaagatc
     9301 tccaacgatc atctggcgaa ggtgaagccc ggcgatgccg aggccatgga ccggctgcgg
     9361 cactattcga tccgctatac cgaggtcatg gcggtcagca gtgatttcgt gctggccgag
     9421 aagggtgact acaagaacga ggccgcccgc cgttggaagg ccttccgcga gcagatcgat
     9481 caggctcgcg ccgccgcggc cgagatggcg acggccggtg ccgccgccag gccatcaccc
     9541 aaggatgcgc ccctgcccat gctgcggatc acggagtctc ccggacctta tagcaacggc
     9601 tcgtagccgc aactcttgac gagccacggg gtttatcccc gtggtcttcg ttgcacgtgc
     9661 agaagaccgg caggatgaat ccgccggcgg cgaacccgcc ggctcgtcaa ggcaagcccg
     9721 gcagggcgtg ttctgcgcgc caacttccgg ccggccgacc gtttgtgccg atcttgctgc
     9781 gtcttggatc ccgaggctcc attcagcccg ccttcggctc cgaggctgat acgtgtttag
     9841 cgtgcaagac agcgggtcag gcactttttt tttcgcgccg ccgagccccc gcctgactga
     9901 gccaaccact ccagattgca cggcgaactg cattggggca aaaagagcca gaccccagca
     9961 cgctaaacac gctctgaggc tgagcctcag gctcgaagag agctcatcgc cgagggcaga
    10021 cccgaaggga gcaaggtcga gggagccgtg ccgaggggct gatcctgatc gtatccatcc
    10081 ggcccgcaga tcatgggatt gacgcagatg gaaaacaagt ggggccgacg ccctcgtcgg
    10141 ccctttctct cgcccggtac cactggttgc cggcccgccc gtgcccgatg ctcaaaca
//
LOCUS       DAOTPW010000210        10171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6174_length_10171_cov_5.167260, whole genome shotgun sequence.
ACCESSION   DAOTPW010000210 DAOTPW010000000
VERSION     DAOTPW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6174_length_10171_cov_5.167260"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="P5316_16670"
     CDS             complement(<1..967)
                     /locus_tag="P5316_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86647.1"
                     /translation="MRTTNLRTLAVVLICCCPAPAACAAEAVDVGSRLELFVDGRLID
                     KVDGARLQLNRPVPREVVFRFDAPWEGGQSGYVTMLQDGDVYRMYYRGGGDLGREYTC
                     LAESKDGITWARPRLGLFPVDGSKANNVVWTGEKKAYWESHNFSPFKDTNPSALPAQR
                     YKAVTLGRATPPGEKDDRKVLFAFASPDGVHWKRLGARPIITEGSFDSHNCAFWDSNR
                     EEYVCYLRQGIKGKRGVCRATSKDFTNWTQPVPLDYGDAPLEHFYTNGVVPYFRAEHI
                     YLGFPMRFVPPQERSTVGFDNRKTDGLSDAVFMAGRDGLHFERLFM"
     gene            complement(1015..2547)
                     /locus_tag="P5316_16675"
     CDS             complement(1015..2547)
                     /locus_tag="P5316_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86648.1"
                     /translation="MVHRWMGPAAARLVLVLLGCAASAEGGGTVVHLKPGVAQLFIDD
                     ELIESQQGLERRLHCPVKDEGGNEPIIAARPGTTILAYGTIVFDTRLKKYVMFSQGFV
                     ERNMYRFTSKDGLNWDTGEGGEFEEITLGPPPEKEPGAIGKPGIDVFSCFYDTRDGEW
                     PYKGWLYYANYGPNREGIYYVRSQDGRKWDRLNLVVNAWAGPGDTSARRIEQDGKEVY
                     GPGDVTLFYHDPIDNRFLGIFKFFTSEKVGRAGNNLRSRAYHFFDRLDEPFEGDRIDR
                     IALLPPAEAKDNNGPADEFYASTAWRYESLWLGGLKVWHGKDDYPYSESGCAFFKLVV
                     SRDGLNWHKVSFKNDTGVPEVFIPNGPQGGNNGRNDGGYMSEFSQGPLRIGDELIYYY
                     SASSWGKNAPPDKRLFGGGIFRARLRIDGFVSVVGGTLTTKPLVTTGNDLTINATGPV
                     SVAAIGADGRILGEARVTGDSLRHEIRFEGKTLGDLAGGKAVRLRFSVMPPGHLYSFT
                     VR"
     gene            complement(2651..3856)
                     /locus_tag="P5316_16680"
     CDS             complement(2651..3856)
                     /locus_tag="P5316_16680"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR86649.1"
                     /translation="MYPTLNQMLDMLSVDSARIEVGRRRQEAVWRGQAPDRVPILLGR
                     SESRQVMEKVGPQHLRLCEHQLRGGRPVREYHRFDHYTLGEQFDDPEKMLIESLWDII
                     GWARTPSDAQLSLRPNFGVGTLASVFGCKTAMGEHDMPWVSERPSKEAILEVDLDHLD
                     RAGLIPRVIEFIQMARSALQEFPDVHVFMPDLQGPMNTAFLLREQEVFIEMLEEEDYF
                     HRLMRIVTEVFVRLARRFKREVGEPLDGGYHGAMYMTNGGVRVVDDVSIMLSPEHYLR
                     YSLPYVRQCLAPFGGGWVHSCGNISHQLDAYLSAPEIKGINFGEPEYYDFADLFPRLA
                     AAGKFCYGGPVREMSESVEAYLERTASALGLARACLIFQPRVRGQDMSEGDWPDPEAI
                     LSLWDRLLS"
     gene            complement(3967..5376)
                     /locus_tag="P5316_16685"
     CDS             complement(3967..5376)
                     /locus_tag="P5316_16685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR86650.1"
                     /translation="MADRSDTGGAAEPRLAWYQGVAPYQWWVFSVGAMAWLFDCMDQR
                     LFTMVRSPALSQLLDLPEKNALVVDYGTYATAAQMIGWAVGGLFFGVVGDRWGRVKTL
                     SASILVYSLFTGLCGLAITGWDFCLYRFLMGCGIGGAFAAAATLIAETMPDHSRSTCL
                     GLFSALSVLGNMLGLAIGSKVFDPAKLYFEGFPGSDGVPGWRLAFFVGTIPALLVVLV
                     LQTLRESERWQQARKKAGENLERQLGDLGSMFRHPRWRTNTIVAVCLATAGIVGVWGV
                     AFWSPELINYALTPQGGLESLPRDQWAAKTAEITSVKFWGNLFQDIGGFLGIASFAPI
                     ANRIGRRKTFAGAFIISFVLIAFVFLTLNSAAKAYVLLPIMGYFNLSVMGGFVVYFPE
                     IFPTRLRSTGTAFGYNVARLSAAVVMLSSNQIREWLAALEFEHPFRVGAVVVSGVYFL
                     GLLVLIWAPETKDRPLPEE"
     gene            complement(5407..6672)
                     /locus_tag="P5316_16690"
     CDS             complement(5407..6672)
                     /locus_tag="P5316_16690"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR86651.1"
                     /translation="MDSEASEWILGPANDGNPPWGGPQNGFWGQRMDSGASEWILGPA
                     SLKAVGRALPAKSFAGGDVSRSAPLFSIDERVDRFRRYYARENERPLLGFYVGSEYPL
                     FRYPAARSLPGDRPLRPDDFEVAPYLDDCERLFGEHEACGGDFIWSASAYWGIPWLEA
                     ALGCPIIADHNTGSIRSEPPAEFAGPERIPEFDPKALWMRKAVEYIDRIADRSAGRWP
                     IGTTRMRGIADLLSALYGGDAFIYAMMDRPEEVRQVARRLTDFWIAFGRMQLERIPLF
                     YGGVGSFYYHMWAPAGTIWHQEDAAALLSPDLYDEFIREHDQRIAESFDGCIMHQHPT
                     AFVPTKFYLEMPFIALELHIDEGGPDAETLEPVHRKILARKPLLIWGHLSEQDLDRIF
                     GRLPAAGLAVMTCVDGPEQAGRLWRRCIG"
     gene            complement(6986..7747)
                     /locus_tag="P5316_16695"
     CDS             complement(6986..7747)
                     /locus_tag="P5316_16695"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRR86652.1"
                     /translation="MKNKLRRALLARELSLGGWMQIGHPACAEIFARAGFDWVCVDLE
                     HGAIDLETTAHIFRTIDGFDCVPVARLPLNDPVWIHRVLDAGARGLIVPMVKTAAEAE
                     AAIRESKYPPRGVRGYGYSRANMHGMDFAEYIVSANEEIAMVMQIEHKDAIANIDAIL
                     EVPGVDGVFIGPLDLSGSMGITGQLDHPQMVAALDRYRAACKSHKVSAGTHIVRPNEQ
                     NIRRAIDEGYTLIALGLDNVFLDEGSRASLKAAGR"
     gene            complement(7747..8748)
                     /locus_tag="P5316_16700"
     CDS             complement(7747..8748)
                     /locus_tag="P5316_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR86653.1"
                     /translation="MNMIKVAVTATEFDKAAEVFRQAARVGVECLRAPAAEAELAVFV
                     RAQGVRHVIVGVEKYTGVLYEALPAGAVLARFGVGHDGIDKDKATARGVICTNTPGVL
                     DDSVAEMTIALILAAARHVAKVAAEVKKGGFAPMVGSELAGKTLAVIGCGPIGRRVAR
                     IAARGFGMRVVGCDVADLDIERMKTEYGFAETGKDYGAAVATADFVSLHIPSTPATRH
                     FMNRERLAMIPAGAWLINTARGAVLDEAALFDALSEGRPAGAALDVFEAEPYAPVAPE
                     KDLRKLDNVVMTPHVGSSTREACNRMAERALQNVRLAEAGRFGEMDLLNPAVIGGRS"
     gene            8972..9994
                     /locus_tag="P5316_16705"
     CDS             8972..9994
                     /locus_tag="P5316_16705"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86654.1"
                     /translation="MIGVGLIGAGFIGRNHFNQYEKMGRRAKVVALCDKEPQRRAGDW
                     SGVGGNIADAQGTKRDLGDIKQYAEWRDLLADPAVQMVDICVPTYLHPEMTIAALDAG
                     KHVLCEKPMALTVEECDKMLTAAVKAKGRFMIAQCIRFWPECVYLKQAIDDKRFGRLK
                     ALTLRRQAATPTYSLNNWLLDPKLSGGAVLDLHVHDTDWALHLLGTPQTITAQICERN
                     GSPDRVHSLWYYRPDLVVQIEGFWDMPPAFGFNMGFTARFEKAALLFDLSSGKPLTIL
                     KDDGSTETPAMGPNDGYYNEIDYFLGCIERGVGPTISTPGESREAVRMALLEKESARS
                     GQAVRV"
BASE COUNT         2104 a         3493 c         2819 g         1755 t
ORIGIN      
        1 ccatgaacag gcgctcaaaa tgcaggccgt cgcgcccggc catgaatacc gcgtctgaca
       61 agccgtccgt cttccgatta tcgaacccga ccgtgctgcg ttcctgcggc ggtacgaacc
      121 gcatcggaaa ccccagatat atgtgctcgg cgcggaaata aggcacgacc ccgttagtgt
      181 agaaatgctc caaaggagcg tccccgtagt ccagcggcac gggttgggtc cagttggtga
      241 aatccttgga cgtggcccga cagacgccgc gcttgccctt tatgccctgg cgaaggtagc
      301 aaacgtactc ctcgcggtta gagtcccaga acgcacagtt gtgcgaatcg aacgagccct
      361 cggtgatgat cggccgcgcg ccgagccgtt tccagtggac accgtccggc gacgcgaagg
      421 cgaaaagcac cttgcgatca tccttctcgc cgggcggagt cgcccttccc agggtgaccg
      481 ccttgtatcg ctgcgcgggc agtgccgaag ggttggtgtc cttgaacggc gagaagttgt
      541 gagattccca gtaagctttt ttctcaccgg tccagacgac attgtttgcc tttgagccgt
      601 cgacggggaa aaggccaagc ctgggccgtg cccaggtgat gccgtccttg ctctcggcca
      661 gacacgtgta ctcccgcccc aggtcgcccc cgccgcgata atacatgcga tacacgtcgc
      721 cgtcctgcag catggtgacg tagccgctct ggccgccttc ccagggggcg tcgaagcgaa
      781 acacgacttc gcgaggcaca ggccggttca actgcagccg agcaccgtcc accttgtcaa
      841 tcagacgtcc gtcgacgaat agttcaaggc gcgaaccgac gtccacggcc tcggcggcac
      901 acgcagccgg agccggacaa caacagatca gcacaactgc cagggtccta aggttcgtcg
      961 ttctcatgcg gttgctctcc agccatgatc gtctgtcaat aggcacggcg gctctcagcg
     1021 caccgtgaac gaatagagat gaccgggcgg catcacgctg aaccgcagcc tgacggcttt
     1081 gccgccggcc aggtcaccca gcgtctttcc ctcgaatcga atctcgtgcc gcaatgagtc
     1141 acccgtcact ctcgcctcgc cgagaattct gccgtcagca ccaatcgcag ccactgaaac
     1201 cgggccggtc gcattgatgg tcagatcgtt gcccgtcgtc accagcggtt tggtggtcag
     1261 ggtgccgcca accaccgaca cgaatccatc gattcgcagc cgggcccgaa agatgccgcc
     1321 gccgaagaga cgcttgtccg gcggagcgtt cttgccccac gaggacgcac tgtagtagta
     1381 aatgagttcg tcgccgatcc gcagggggcc ctggctgaac tcgctcatgt acccgccgtc
     1441 attgcggcca ttgttgcccc cctgcgggcc gttcgggatg aatacctcgg gcacgcccgt
     1501 gtcgttcttg aatgagacct tgtgccaatt caacccgtca cggctgacaa ccagcttgaa
     1561 gaacgcgcac ccgctctcgg aatacgggta gtcatccttc ccgtgccaga ctttcagccc
     1621 gccgagccac aaagactcgt atcgccaagc cgtactggca tagaactcgt cggccggccc
     1681 gttgttgtcc ttcgcctcgg ccggaggcaa aagggcgatg cgatcgatac gatcgccctc
     1741 gaacggctca tccagccgat cgaaaaagtg gtacgcccga ctgcgaaggt tgttgcccgc
     1801 gcggcccacc ttctcgctgg taaaaaattt gaagatgccg agaaaacggt tgtcgatcgg
     1861 gtcgtgatag aagagcgtca cgtccccggg accatacacc tccttgccgt cctgttcgat
     1921 ccgacgggca ctggtgtcac ccgggccggc ccaggcgttt accaccaggt tgagacggtc
     1981 ccatttgcgg ccgtcctgcg atcgcacgta gtagatgcct tcgcgattcg gaccatagtt
     2041 ggcgtagtag agccagccct tgtacggcca ttcgccatcc cgagtgtcgt aaaagcatga
     2101 gaaaacgtca atcccgggct tgccgatcgc gcccggttcc ttctccggag gcggacccaa
     2161 ggtgatttcc tcgaattccc cgccctcgcc ggtgtcccag ttcagcccgt ccttggaggt
     2221 gaagcggtac atgtttcgct cgacgaaacc ctgcgagaac atgacatact tcttgagccg
     2281 ggtgtcgaac acgatggtgc cgtaagccag gatcgttgtc cccggcctcg cggcgatgat
     2341 cggttcattc ccgccctcgt ccttcaccgg gcagtgaagg cgacgttcaa gcccctgctg
     2401 cgactcgatc aattcgtcat cgataaacag ttgagcgacc cccggcttga gatgaacgac
     2461 cgtgcctccg ccctcagccg aggccgcaca ccccaacaag accagaacaa gcctcgcagc
     2521 agccggaccc atccaccgat gcaccatggc aacacctccc gtaccagacg gccacggagc
     2581 gccagtctac aaagatgagc cattcctgcc aacaggaatc gcgtgaaaag cctgacattc
     2641 cgacacgggt tcaactcaaa aggcggtccc acaaagacag gatcgcctcc ggatcgggcc
     2701 agtcgccttc cgacatgtcc tgaccccgaa ctcggggttg gaagatcagg caagccctcg
     2761 ccaaacccag agctgaggcc gtacgctcga ggtaagcctc gaccgattcg ctcatctccc
     2821 gcaccggccc gccgtaacag aacttcccgg ctgcggccaa tcgcggaaac aggtccgcga
     2881 aatcatagta ctccggctcg ccaaagttga ttcccttgat ctctggcgca ctgagataag
     2941 catccagttg gtgcgagatg ttgccgcaac tgtgaaccca tccgccgccg aacggggcca
     3001 ggcactgccg gacgtatggc agcgaatacc gcaagtagtg ctcgggtgaa agcatgatgc
     3061 tcacgtcatc gaccacgcgg acgccgccgt tggtcatgta catcgccccg tgatagccgc
     3121 cgtccagcgg ctcgccaacc tcccgcttga agcgcctggc gaggcgaacg aaaacctcgg
     3181 tgacgatccg catcagccga tgaaagtagt cctcctcttc aagcatctcg atgaacactt
     3241 cctgctctcg aaggagaaaa gcggtgttca tcggcccctg aaggtcgggc atgaaaacgt
     3301 gaacatcggg gaactcctgc agagcactgc gggccatctg gatgaactcg atcacccgcg
     3361 ggatcaaacc cgcacgatcc agatggtcga ggtcgacctc cagaatcgcc tccttcgacg
     3421 gccgttcgct cacccagggc atatcatgct cccccatggc cgtcttgcat ccgaagacgc
     3481 tcgccagagt gcccacgcca aagttcggcc gcagcgagag ctgcgcatcg ctcggcgtgc
     3541 gggcccagcc gataatgtcc cacagcgatt cgataagcat cttctcagga tcgtcaaact
     3601 gctctccgag tgtgtagtga tcgaagcgat gatactcccg aaccggccgt ccgccgcgaa
     3661 gctggtgctc gcacaaacgc aggtgctgag gcccgacttt ctccatgacc tgccgcgatt
     3721 cgctgcggcc cagcagaatc ggcacgcggt ccggggcctg tccccgccag acggcctcct
     3781 gacgtcgccg gccgacctcg atccgggccg aatcgacgct gagcatgtcg agcatctggt
     3841 tcagcgtagg gtacatgttg aattggctct ccagtatgtc cgaatctccg tccgacagaa
     3901 cccggatccc cggcgacagg cccggaaccg ctgccgcccg ctgtccctcg gcctctctga
     3961 agaagcctac tcctccggca acggccgatc cttggtttct ggagcccaga tcagcaccag
     4021 cagacccaga aagtaaacgc cgctgaccac caccgccccc acgcgaaacg gatgctcaaa
     4081 ctccagagca gcaagccatt cgcgaatctg attactcgac agcatgacaa ccgccgccga
     4141 cagacgggcg acgttgtacc cgaacgcagt accggtcgat cgcagccgcg tcgggaagat
     4201 ctcaggaaaa tagaccacaa acccccccat cacgctcagg ttgaaataac ccatgatcgg
     4261 cagcaggaca tacgctttcg ccgccgaatt cagcgtaagg aacacgaagg caatcaacac
     4321 gaacgagatg atgaaggccc cggcaaaggt cttgcggcgg ccgatccggt tcgcgatcgg
     4381 ggcaaaactg gcaatgccaa gaaaaccgcc gatgtcctgg aacagattac cccagaactt
     4441 gaccgaggtg atctccgccg tcttggcggc ccattggtcc cggggcagac tctccaaccc
     4501 tccttgaggc gtcaaagcat agttgatcag ctcgggcgac cagaacgcta cgccccacac
     4561 cccgacgatc cccgccgtcg ccaggcaaac cgcgacgatg gtgttggtcc gccagcgcgg
     4621 atggcggaac atgctgccca ggtcgcccaa ctgacgctca aggttttctc cggccttctt
     4681 gcgggcctgt tgccatcgct cggactccct gagcgtttgc agcaccagca ccaccagcag
     4741 agccggtatc gttccaacaa aaaaagcgag acgccagccg ggcacgccat cactgcccgg
     4801 aaacccctcg aagtagagct tggccggatc gaagaccttc gagccgatgg ccaggcccag
     4861 catgttccca agcacggaca aggccgaaaa aagccccagg cacgtcgagc gggaatgatc
     4921 cggcatcgtc tcggcaatca gcgtggcagc agcggcaaac gcccccccga tcccgcatcc
     4981 catcagaaaa cggtacagac agaaatccca gcccgttatc gccagaccgc aaagaccggt
     5041 gaacagcgag tagaccagaa tcgacgccga caacgttttg acgcgccccc aacggtcgcc
     5101 gacaacgccg aagaacaaac caccgacggc ccatccgatc atctgcgccg ccgtcgcata
     5161 agtcccgtag tccaccacga gcgcgttctt ctccggaaga tcgagcaact gcgacagggc
     5221 cggcgatcga accatcgtga acagtcgctg atccatgcaa tcaaacagcc aggccatcgc
     5281 cccgacactg aacacccacc actggtacgg ggcaacaccc tgataccacg caagtctcgg
     5341 ttcagcagcc ccgcccgtat ccgatcgatc ggccatccca tacctcccga gaacaactcg
     5401 gccgtctcag ccgatgcacc ttcgccacaa ccgcccggct tgctccggac catccacgca
     5461 ggtcatcact gccagtccgg ccgccggcag gcgcccgaaa atacggtcca agtcctgctc
     5521 gcttaaatgc ccccagatca acagcggctt gcgcgcgagg atcttgcggt gcaccggctc
     5581 cagagtctcg gcatccgggc caccttcgtc aatatgcagc tccagagcaa tgaagggcat
     5641 ctccaggtag aacttcgtcg gcacaaacgc cgtcgggtgc tggtgcatga tgcagccgtc
     5701 gaacgattca gcgattcgct ggtcgtgctc gcgaatgaac tcatcgtata aatccggcga
     5761 caataacgcg gccgcgtctt cctggtgcca gatggtcccg gccggggccc acatgtggta
     5821 gtagaacgac ccgaccccgc cgtaaaacag cggtatccgc tcaagctgca tccgcccgaa
     5881 cgcaatccag aaatcggtca gccgccgggc aacctggcga acctcctccg gccgatccat
     5941 catcgcgtag atgaaggcat ctcccccgta gagagccgag agcagatcgg cgatgcccct
     6001 catgcgagtg gtgccgatcg gccagcgtcc cgcgcttcgg tcggctatgc gatcgatgta
     6061 ctcgaccgcc tttcgcatcc ataaggcctt cggatcgaac tctggaatgc gctcaggtcc
     6121 ggcgaattcc gccggcggct ccgagcggat tgaacccgtg ttgtggtcgg cgatgatcgg
     6181 acagcccaac gccgcctcca gccacggaat gccccaatac gcactggcgc tccagatgaa
     6241 atctccgccg caggcctcgt gctctccgaa caatcgttca caatcatcaa ggtatggggc
     6301 gacctcaaaa tcatcaggcc tcagcggtcg gtcaccgggc aacgatcgcg ccgccgggta
     6361 gcgaaacagc ggatactcgc tgccaacgta aaaccccagc agcggccgct cgttctcgcg
     6421 ggcatagtac cggcgaaacc gatccacgcg ttcgtcaatc gagaatagcg gagcacttcg
     6481 agacacatcg ccccccgcga aagacttggc gggcaaagcc cgccctaccg ccttcaggct
     6541 cgctggcccc agaatccatt cgctggcccc agaatccatt cgctggcccc agaatccatt
     6601 ctggggccct ccccatggag gattcccatc attcgctggc cccagaatcc attcgctggc
     6661 ctcagaatcc attctggggc cctccccatg gaggatgccc attcgctggc cccagaatcc
     6721 attcgctggc cccagaatcc attctggggc cttccccatg gaggattccc attcgctggc
     6781 cccagaatcc attcgctggc cccagaatcc attctggggc cttccccatg gaggattccc
     6841 attcgctggc cccagaatcc attctggggc cttccccatg gaggattccc atcctcattc
     6901 ctgccgcacg cggaaaaacc ccgccacgcg aggcggcccg gaagggatgc cgggccgagc
     6961 aggtccctgg cagtcaaagc ccgccctacc gccctgccgc cttcagactg gccctcgatc
     7021 cctcgtcgag aaacacgttg tccagcccca gggcgatcag cgtgtagccc tcgtcgatcg
     7081 ccctgcggat attctgctcg ttcggccgga cgatgtgcgt gccggctgat accttgtggc
     7141 tcttgcaggc agcccggtac ctgtcgagcg ccgcgaccat ctggggatgg tcgagctggc
     7201 cggtaatgcc catcgagccg ctcaggtcca gcgggccgat gaacacgccg tcgacgcccg
     7261 gaacctccag aatcgcatcg atgttggcga tggcatcctt gtgctcgatc tgcatcacca
     7321 tggcgatctc ttcgttggcc gagacgatgt actcggcgaa atccataccg tgcatgttcg
     7381 cccggctgta accgtacccg cgcacgcctc gcggtggata cttgctctcg cggatcgcgg
     7441 cctcggcctc cgcggcggtc ttgaccatcg ggacgatcag cccgcgggcc ccggcatcca
     7501 ggacccggtg aatccacacc ggatcgttca gcggcaaccg cgccaccggc acgcaatcaa
     7561 aaccgtcgat cgtgcgaaaa atgtgcgccg tcgtctccag gtcgatcgcc ccatgctcca
     7621 ggtcaacgca aacccaatcg aatcccgccc gggcaaagat ctcggcacag gccgggtgcc
     7681 cgatctgcat ccatccaccc aacgacaact ctcgagcaag cagcgcccga cgcaacttgt
     7741 ttttcatcac gacctccccc caatcacggc cgggttaagc agatccatct cgccgaaacg
     7801 gccggcctcg gccaaccgta cgttctgcag cgcccgctcg gccatgcggt tacacgcctc
     7861 gcgggtgctg ctgcccacgt gcggagtcat cactacgtta tccagcttgc gaagatcctt
     7921 ctccggggca accggcgcgt acggctcggc ctcgaatacg tccaatgccg ctcccgccgg
     7981 cctgccttcc gacagggcgt caaacaaagc cgcctcatcg agcaccgcgc cgcgcgccgt
     8041 attgatcagc cacgccccgg ccgggatcat cgccagccgc tcgcgattca tgaagtgccg
     8101 cgtggccggc gtgctcggaa tgtgcaggct gacgaaatcg gcggtcgcaa ccgccgcacc
     8161 gtaatccttg cctgtttcgg cgaatccgta ttccgttttc atccgctcga tatccaggtc
     8221 ggccacatcg catcccacga cgcgcatccc gaaccctcgg gccgcgatcc tcgcgactcg
     8281 tcgcccaatg ggtccgcagc cgatcacggc cagcgtcttg cctgccagct cgctacccac
     8341 catcggcgca aacccgccct tcttgacttc cgccgcaacc ttcgccacgt gccgcgccgc
     8401 cgccagaatc aaggcgatcg tcatctcggc cactgaatcg tcgaggacac cgggcgtatt
     8461 ggtgcagatc acgcctcgag cggtcgcctt gtccttgtca atgccgtcgt gtcccacacc
     8521 gaagcgggcg agcactgcgc ctgccggcaa cgcctcgtac agcactccgg tgtacttctc
     8581 cacgccgaca atgacatgac gcaccccctg agcacggaca aagaccgcca gttcggcctc
     8641 cgccgcaggg gctctcaggc attccacgcc gacacgcgca gcctgccgga atacctccgc
     8701 cgccttgtcg aactccgtcg ctgtgaccgc aactttgatc atgttcatcc cgacgtcggt
     8761 gcccccgaaa gtcctgcgga aacatctgac aaccgcccgg ccattcatcc cccgaacggt
     8821 tctcgcatcg tcaggcgagg cgctgtagag tatcgtgcca tgcggcaaac gccaagcgct
     8881 tggggcgcgg cgataagaaa ggagccgagg cacgctgcga cagcgtgaag agaatctgcc
     8941 ctctcgcctc tcgaatgcaa ggagattcat tatgatcgga gtcggtctca tcggagccgg
     9001 gttcatcggg cgaaaccact tcaaccagta cgagaagatg ggccggcggg ccaaggtcgt
     9061 cgcgctctgc gacaaggaac cgcagcgccg cgccggagac tggtcgggcg tcggaggcaa
     9121 catcgcggat gcccagggaa ccaagcggga tcttggtgac atcaagcaat atgccgagtg
     9181 gcgggatctg ctcgccgacc ccgccgtaca gatggtcgac atctgcgttc ccacttatct
     9241 gcatcccgag atgaccatcg ccgccttgga cgccggtaag cacgtcctgt gtgagaaacc
     9301 catggccctg accgttgaag aatgcgacaa gatgctgacg gcggccgtca aagcgaaggg
     9361 acgcttcatg atcgcccagt gcatccgctt ctggcccgaa tgcgtctacc tcaagcaggc
     9421 catcgacgac aagcgattcg gcaggctcaa ggctttgacc cttcgccgcc aggccgccac
     9481 ccccacctat tcactcaaca attggctcct cgatccaaag ctgtcgggcg gagcggttct
     9541 tgatctgcac gtccacgaca cggattgggc cctgcacctg ctcggcacgc cccagacgat
     9601 caccgcccag atatgtgaac gaaacggatc tcccgaccgc gtgcattcgc tgtggtacta
     9661 tcggcccgat ctcgtcgtcc agatcgaagg cttctgggac atgccgcccg ccttcggctt
     9721 caacatgggc tttaccgccc gcttcgagaa ggccgccctg ctgttcgacc tcagcagcgg
     9781 caagccgctc acaatcctca aggacgacgg ctccaccgaa acgcccgcaa tgggcccgaa
     9841 cgacgggtac tacaacgaga tcgattactt cctcgggtgc atcgagcgag gagtcggacc
     9901 gacgatcagc actccaggtg aaagccgcga ggccgtgcgt atggccttgc tcgaaaagga
     9961 atccgccagg tccggacagg cggttcgcgt ctgaccacgt atgaaatggg agacctgggt
    10021 ggcgggaagg cccgggcgac gctgacgata gcaggcggtt cgcgtctgac cacgtatgaa
    10081 atgggaggac accgccgcag caagacaaaa cagttgcaaa ccaacgtacg acacggtcgc
    10141 agcaacgtac ggcacggtcg gagcaaggta g
//
LOCUS       DAOTPW010000211        10166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6177_length_10166_cov_5.671941, whole genome shotgun sequence.
ACCESSION   DAOTPW010000211 DAOTPW010000000
VERSION     DAOTPW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6177_length_10166_cov_5.671941"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="P5316_16710"
     CDS             <1..847
                     /locus_tag="P5316_16710"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRR86655.1"
                     /translation="GTAAAVGAGVTGYTSGEPVAVCVVVSCGQCEYCRADRENLCDTR
                     YTLGYATDGSFAEYMLIPALAVSRGNLFHLPPQIPMEMAPLLEPIACCLNGQHEMGLG
                     QDKGVKDGRPQSLLIFGAGPIGLIHLMLAKAKGVRPITVVEPVAHRRELAGRFGADEV
                     VSPDQFVVAARFDAAILAVGVPELVNVALRAVKKTGRISLFAGFSGGATATIDPNAVH
                     YGQITISGASESRRRDFAEAMSLVQRGIIDPALLLTHRFSLDQYEQAFRVAGDGTAVK
                     VIFEI"
     gene            952..2268
                     /gene="ricT"
                     /locus_tag="P5316_16715"
     CDS             952..2268
                     /gene="ricT"
                     /locus_tag="P5316_16715"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRR86656.1"
                     /translation="MGKCGGRASAIDRIGPEAERMQLFTTVRYGKMGLIGEFSYPPNL
                     DFTCGGKLVISTDRGLEIGEHVPLTCTGCERQIRREQIEAYTQASSGDETYRFKNGRI
                     LREATEADLAEFRHIQATTPQKLAIASKLAAEYGLDMKIVDCEQILGGERIILFFMSE
                     QRVDFRQLVRRLASEFQTRIEMRQVGARDEARLLADYETCGRECCCKNFLKTLKPITM
                     SMAKLQKTTLDLAKVSGRCGRLKCCLRYEHETYDALNKKLPRNNSWVRTPAGIGKVMD
                     RQIITQLVRIRMEDDRIVVVPAEEILETNLPAPPPRPPGDERGNGSSSQRDRREAPAP
                     SDRRLLRTLVPKYDEPTEEDVPIEQVQADSPLPEKPGPAVETQPDSLPKGPEAGQKIQ
                     EPSDNLRQQRHGRRGRRSRRRGRRNRPGRGPDGGSSGGTEGGSAKV"
     gene            2271..2789
                     /locus_tag="P5316_16720"
     CDS             2271..2789
                     /locus_tag="P5316_16720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86657.1"
                     /translation="MPQRPKKPIEEVVAELGRYPLEAFIFMQECIGAASEQVHGPMTP
                     EESVVAGWMDRHNVDLETLCRLSETGSLPPDIADILAKIGGPEKMNRHVTGQQLCWAV
                     RDVALSRYGLMARSVLAKWNIRRTEDIGAIIFALVENDWLKKLPSDKIEDFQDVYSFD
                     EVFEPDYRSGGE"
     gene            3099..4439
                     /locus_tag="P5316_16725"
     CDS             3099..4439
                     /locus_tag="P5316_16725"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86658.1"
                     /translation="MNDWLDAESRVERALQLSEAQQWSEALRELEAAIAINPNDPIWH
                     AQRGQILDQLENYDSAVEAYRQALDLDPEHLGIKTALAIDMIRVGRYADAVEVLAEME
                     KIDPNYEPSFCHRVAAYTRMGLHDKAEEMFYLAQQINPDCPNCFHHMAESLACRAEYS
                     KAIFCWQRALEIAPDYPQVRQRIAEAYRAQKLYDKAREGYLAALRHDPGNTELLAELG
                     DLLIEMDDLEGAAAKFQYLVELDPQSVHGYAMLGLIAAQRDDLDRAVRYFESALMLDE
                     ACPGLRAHMGEVELRRGNYYDALHHLSLALEDQPDDCMARMAMGNCLLELGRPEEAAR
                     HFERMIELDSGAVGAHHNLAVCYFLQNDFEAGISHCHEALRLDPGNIMAIHKLALAYL
                     HTCNWTKARQMIQRGLALQPDHAGLKALPKTFIGLRFRQLGRRLIPSWLRGRQR"
     gene            4743..5801
                     /locus_tag="P5316_16730"
     CDS             4743..5801
                     /locus_tag="P5316_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86659.1"
                     /translation="MQRSVFVPAFVAMVVLGVTTALPQDQDQAQASASKKNPYAGSPL
                     TWNVESIIDNYVSHMTRYYNLTPDQEEYTRQLLTQRVKRFLNDYEKDVRSLFAEYWYL
                     QLRGELPSAEEAKDLARRGGPLIAAIKKEILDGNQKWREILDENQRKIHDRDLEQMSK
                     TFDEFEQRLDRWSKGIVDPTDVPRRKVGPRSAPIKPEDAWDYRVSSFIAEYNLDAGQQ
                     GTARSILRELKEEAKRYRERNQEKLTALNDQLKELYASEPKTDPEERKKASEEIKKLN
                     DRLNELEKPIREDLWRQLLTRLDRIPTEDQRRMHREKQERMMARLTGRVSSTRTAASQ
                     PESTVSPATNPAVEASTR"
     gene            complement(5908..6630)
                     /locus_tag="P5316_16735"
     CDS             complement(5908..6630)
                     /locus_tag="P5316_16735"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRR86660.1"
                     /translation="MDEPTDQLRLTCAQCRAIDRYAIEELGIPGVVLMENAGRNAADL
                     IHKWLADLAGNRKRPGRIVIICGRGNNGGDGFVVARHLVHRGHEVWIDLVADPATLAG
                     DAAVNHSIVEKMGLPIRLLTDTAAISEATQHWRQADVIVDALLGTGFTGTVREPLAQV
                     IREVNALSGPLVVAIDVPSGLDADTGHAAGEAVRAHQTITFLAEKSGFACESARAYTG
                     QVTVVDIGAPTSMILSRLAAAR"
     gene            6985..8211
                     /locus_tag="P5316_16740"
     CDS             6985..8211
                     /locus_tag="P5316_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86661.1"
                     /translation="MSQVSRRSLLAASAAAAVIGDFLPKRAEAAESIPGVDQKKTDHD
                     RTKEWKPFSDRKVRVGIVGHGVCQFGLAFDLQNHPNVQLVAVSDLFPDRCADMARKAK
                     CEKTYPSLEEMVKDDSIEAIYCATDAPSHAKHAILCMEHGKHVATAVPAFRGDIEDAE
                     RLLECCRKNKGLVYAMFETSVFHDDLYAMEKLFAAGVFGRIIYSEGEYCHPHSLDAPP
                     LDSYKDWRKNGCPMWYPTHATAYYVGVTHKSFVEVSCQGTPDFDESRRVPNAIGNIFR
                     SEVGLFRTAEGGISRMIVCYSQGEYLEAGRIRGEYGGFNKTFVGDATGKKRYEEALAK
                     GLQIEKYALPPGVEPGGHGGSHGYLGDDFIDAILRGRKPAVDVIDALNMTVPGYYAHL
                     SALKDGETLKIPQYTL"
     gene            8298..9809
                     /locus_tag="P5316_16745"
     CDS             8298..9809
                     /locus_tag="P5316_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR86662.1"
                     /translation="MSTEPPSKSLAAIQGIIDAGRPLIYIQSAEEDRVIGLLSRIAEQ
                     RRGGRVDLFLWSATEGLQQNGKPVKNQPDGPRGVLDFVVTHDKPGLFLLKDFHEFMRD
                     AVEIRRRLRDLYYRCLGTDKFAVICSPVKCIPEEINREVAFIELPRPDLKELEELLRT
                     EAESVRGAGGSAGLSAETIHRLTRAVQGLTFHEARHALRRAMAEHGKLDESILQTLEQ
                     EKQQLVRKTGLIEYVPGTVGVEQIGGLDVLKTWLMERRELFFSRESISSEIVPKGLLL
                     MGVSGCGKSLSARAVANVFCLPLYRIDMVQVFSAGVGNAERLFAGACRAMEEVSPAVV
                     WFDEIENGISRHQQDETGALNRIFGFFLTWMQEKPAGLFVAATANRIDLLPAEMIRKG
                     RFDQVFFIDLPDYDERLEIFKIHLSRRGVDPSTLRLDLIAERTEGWTGAEIEQAVIAA
                     LVSARLAKETVTDEHLFPALRQIVPLAKTMREQINHIRSWAFDRAVHASARKK"
BASE COUNT         2000 a         2850 c         3376 g         1940 t
ORIGIN      
        1 cgggacggcc gccgccgtcg gggcgggagt cacggggtat acgtcgggcg agccggtggc
       61 cgtgtgtgtg gtggtgtcgt gcggccagtg cgagtactgc cgggccgacc gcgaaaacct
      121 gtgtgacacg cgatacaccc tgggttacgc gacggacggg tcgttcgccg agtacatgct
      181 cattccggct ctggccgttt cacgcggcaa cctgtttcat ctgccgccgc agatcccgat
      241 ggagatggcc cccttgctcg agccgatagc ctgctgtctg aacggccagc atgaaatggg
      301 gcttggccaa gataagggcg tcaaggacgg ccgtccccag tcgctgctta tcttcggggc
      361 cgggccgatc ggcctgatcc atttgatgct ggccaaggcc aagggcgtga ggccgatcac
      421 cgtagttgag ccggtggctc atcgccgcga gctggccggg cggttcgggg ccgatgaggt
      481 cgtctcgccc gatcaattcg ttgttgccgc gagattcgac gccgccatcc tggccgtggg
      541 cgttccggag ctggtgaatg tcgcattgcg ggcggtcaag aaaacgggca gaatcagtct
      601 tttcgcaggt ttcagtggcg gcgcaacggc aaccatcgat ccgaatgccg tccattacgg
      661 ccagatcacc atatcaggcg cctccgaatc ccgccgccgt gattttgccg aagccatgtc
      721 cctggtccaa cgaggcatca tcgacccggc tttgctgctg acgcatcggt tctctctcga
      781 tcagtacgag caggcgtttc gcgttgcggg ggatggaacg gcggtgaagg tgatatttga
      841 gatctaatgg ccggtccgat attatgagcg gtcaggccgg ggtcgtccgt cgccgaggct
      901 gacgggcgaa accgttgcct ttgtgcaagc tcagcgaggc agtgtcaacg aatgggcaaa
      961 tgcggcggac gtgccagcgc gattgaccga atcggcccgg aggccgagcg gatgcagctt
     1021 ttcacgacgg tgcggtacgg caagatgggg ctgatcggcg agttctcgta cccaccgaac
     1081 cttgacttca cctgcggcgg caagctggtg atttcgactg atcgggggct tgagattggc
     1141 gagcacgtgc cgctgacgtg caccggatgc gaacgacaga ttcgccggga gcagattgaa
     1201 gcctacacgc aagccagtag tggtgacgag acctatcgat tcaagaacgg tcgtattctt
     1261 cgcgaggcga ccgaagccga cttggccgag ttcagacaca ttcaggcgac cacgccccag
     1321 aaactggcca ttgcctcgaa gctggccgcg gaatacgggc tcgatatgaa gatcgtggat
     1381 tgcgagcaga tcctcggcgg cgagcgaatc atcctgtttt tcatgtccga gcagcgggtt
     1441 gatttccggc aactggttcg acggctggca tccgagtttc agacgcggat cgagatgcgg
     1501 caggtcgggg cgcgcgacga ggcgcggttg ctcgcggatt atgaaacgtg cgggcgagag
     1561 tgctgctgca agaactttct gaagactctc aagcccatca ccatgtccat ggccaagctc
     1621 cagaagacta cccttgacct ggcgaaggtc agcggtcggt gcggacggct caagtgctgc
     1681 ttgcgatacg agcacgagac atacgacgcg ctgaacaaga agttgccgcg caacaacagt
     1741 tgggtccgca cacccgcagg catcgggaag gtcatggatc gtcagatcat cacccagttg
     1801 gtgagaatcc ggatggagga cgaccgcatt gtcgtcgttc ctgccgagga gatcctggag
     1861 acgaacctgc cggcgcctcc gcccaggccg cctggggatg agaggggtaa cggttctagt
     1921 tctcagcgag atcgtcgcga ggcgccggcc ccatcggacc gccggctgtt gcgcacactc
     1981 gtgcccaaat atgatgagcc gaccgaggag gatgttccga tcgagcaggt tcaagcggac
     2041 tcgcctcttc ccgaaaagcc aggcccggct gtcgaaaccc agccggacag tttgcccaag
     2101 ggcccggagg ccggccagaa gatccaggaa ccatccgaca atctgcggca acaacgtcat
     2161 ggccgacgag gccgccgatc gcgtagacgt ggtcgacgaa accggccggg gcgtggcccg
     2221 gacggcggat cgagcggcgg gacggagggc ggttcagcca aggtctagcg atgccccaac
     2281 gaccgaagaa acccattgaa gaggtcgtcg cggagttggg ccgctatccg ttagaggcct
     2341 tcatcttcat gcaggagtgc atcggagcgg cctcggagca ggtccacggg ccgatgaccc
     2401 ccgaggagag cgtcgtggcc gggtggatgg atcggcacaa cgtcgatctg gagacgcttt
     2461 gtcgcttgag tgaaaccggt tcgcttcccc cggatatcgc ggacattctt gcgaagatcg
     2521 gggggccgga aaagatgaac cgccacgtga ccggccagca gctatgctgg gcggttcgcg
     2581 acgttgccct gagccgctac gggctgatgg cccgaagcgt cttggccaag tggaacatca
     2641 ggcggacaga ggacatcgga gcgatcattt ttgcgctggt cgagaatgac tggctgaaga
     2701 agctccccag cgacaagatc gaggatttcc aggacgtata ctcgttcgat gaggtatttg
     2761 aacctgatta ccggtctggg ggtgaatgac gtattgtcgt caccctcttg aggtccccac
     2821 attcacgtcc tgtgcccgtt cgtttggtaa ggcgagcgtc gccaccgctg cgaggacaca
     2881 gtcggcaacg ggtcttcgtc tcggtttgtg aaataaccga ggttggaagc agccatgcgg
     2941 ctcaggcgac gggaaccagg gatgccccat aactgccgcg acggatgggc acaaacctgg
     3001 agatgatttc tgtccggttt accagaggac gcgtcgacac caaccgatat ctatagcgca
     3061 ccaatgacca cccggcgaga tatcggggtg ctgagacgat gaatgattgg ctggatgccg
     3121 agagtcgcgt ggagcgggcg ctgcagctta gcgaggcgca acagtggagt gaggccttgc
     3181 gggagctgga ggcggccatc gccatcaacc cgaacgatcc gatctggcat gctcagcgag
     3241 gtcagattct ggaccagctt gagaattacg actcggcggt agaggcgtat cgtcaggcgc
     3301 ttgatcttga cccggaacac cttggcatca aaacggcctt ggccatcgac atgattcgcg
     3361 tcggacgata tgcagacgcc gttgaagtcc tggcggagat ggagaagatc gacccgaact
     3421 acgagccgtc gttttgtcac cgggtcgcgg cgtacacgcg catgggcctg cacgacaagg
     3481 ccgaggagat gttctatctt gcccagcaga tcaaccccga ctgtccgaac tgcttccacc
     3541 acatggcgga atcgctggct tgccgggcag agtactctaa ggccatcttc tgctggcaga
     3601 gggcccttga aatcgcgccg gattatcctc aggttcgcca gcgtatcgcg gaggcctacc
     3661 gggcgcagaa gctttatgac aaggcccgcg aggggtactt ggcggcgctg cggcacgatc
     3721 ccgggaatac cgagctgctt gcggaactgg gtgacctgct gatcgagatg gacgatctgg
     3781 agggtgctgc ggcgaagttt cagtacctgg tggagctgga tcctcagtct gtgcacggtt
     3841 acgccatgct ggggcttatt gccgcgcagc gggacgactt ggacagggcg gttcgttatt
     3901 tcgagtcggc tctgatgctg gacgaggctt gccccggcct gcgggcgcat atgggcgagg
     3961 ttgagcttcg tcgcggcaac tactatgacg ccctccacca tttgtccctg gcgttggagg
     4021 accaaccaga cgactgcatg gcgcggatgg ccatgggcaa ctgccttctg gaactgggtc
     4081 gtccggagga ggcggcgcgc cactttgagc ggatgatcga actggacagc ggcgccgtgg
     4141 gagcccatca caatctggca gtctgctatt ttctccaaaa cgacttcgag gcggggatca
     4201 gtcactgcca cgaagccctg cggctcgatc cgggcaacat catggccata cacaagctgg
     4261 ccttggccta tctgcacacc tgcaactgga cgaaagcccg gcagatgatt caacggggct
     4321 tggcgctgca accggaccat gcgggtctca aggccctgcc caagaccttc attggtcttc
     4381 gctttcggca actcggccgg cggctcatcc cttcctggct acgcgggcgg cagcgctgat
     4441 cgagggccgc tcttgccctg cgggcagatc atcggttagc atcaattcca gcgtattcgg
     4501 agccaagtga gcctttaagc cctgtggttt cgttccggct tgaattgttg tctgcaacct
     4561 ccttgggggt tggcaatata agttaggtgc gggggcgggg ctcagcctga gtctaggatc
     4621 gcgcccgtcg ggcggacaac gagaagtgcc tccgcacccg ccaatggtga accagggatg
     4681 agaggttgcc ggccgagagg gagatgcagg cccgcacctt ggaactacac gaggacagat
     4741 acatgcaaag aagcgttttc gtcccggcgt ttgtggccat ggtggtcttg ggggtgacga
     4801 cggccttgcc ccaggatcag gaccaggcac aagcatctgc gtcgaagaag aatccttatg
     4861 ccgggtctcc gctgacgtgg aacgtcgagt cgatcatcga caattatgtc agccacatga
     4921 cccgctacta caaccttacg ccggaccagg aggagtacac ccgccaactc ctgactcagc
     4981 gggtcaagcg gttcctgaat gactacgaga aggatgtccg gtcgctgttc gccgagtact
     5041 ggtacctcca gctacgaggc gaactgcctt cggcggaaga agccaaggat ctggcacggc
     5101 gtggaggacc gctgattgct gccatcaaga aagagattct ggacggaaac cagaaatgga
     5161 gagagatcct cgatgagaac cagcggaaga tccatgaccg cgatctcgaa cagatgagca
     5221 agacgttcga cgagtttgag cagaggctgg atcggtggag caaaggcatc gtggatccga
     5281 ccgacgttcc tcggcgcaag gtcggacccc gttctgcacc gatcaagccg gaggacgcat
     5341 gggactatcg ggtgagttcg ttcattgccg agtacaactt ggacgccggt cagcagggca
     5401 cggcccgctc gattctgagg gagctgaagg aggaggcgaa acgttaccgt gagcggaacc
     5461 aggagaagct gacggcgctc aacgaccaac tcaaagagct gtatgcctcg gagccgaaga
     5521 ccgaccctga agagaggaag aaagcctcag aggagattaa gaagctcaac gaccggctca
     5581 acgagttgga gaagccgatc agggaggact tgtggcggca actgttgacc cgcctggatc
     5641 gcattccgac ggaggatcaa cggcggatgc acagggagaa acaggagagg atgatggctc
     5701 gtttgacggg gcgggtttct tcgaccagaa ccgccgccag ccaaccggag tcgactgttt
     5761 ccccggcgac caaccctgcg gtggaggcca gtacgcggta atcgacatct gggacgggta
     5821 agcgcaacca ctcacgacgg cggccggcgt tgtgccggcc gttgttttgc gcgacagggg
     5881 gcaatgaacc ggtcgcgtct ggtgcggtca tcgggcggca gcgaggcgcg aaaggatcat
     5941 cgaggtggga gcgccgatgt cgaccaccgt gacttggccg gtatatgccc gggccgattc
     6001 gcaggcaaat ccgcttttct ctgccaagaa agtgatagtc tgatgagcgc ggacggcttc
     6061 accggccgcg tggccggtgt cggcgtccag acccgagggc acgtcgatcg cgaccaccag
     6121 cggtccggac agggcgttga cctcccggat gacttgggcc agcggttcgc gcaccgtccc
     6181 ggtgaacccc gttcccagca gggcatcgac gatcacatcg gcttgccgcc agtgctgggt
     6241 cgcttccgaa atggcggcag tatcggtcag cagccggatc ggaaggccca tcttctcgac
     6301 aatggaatga ttgactgccg cgtcgcccgc gagtgtggcg gggtcagcca ccaggtcgat
     6361 ccacacctcg tgtccgcgat gcaccaggtg gcgggcgacg acaaagccgt cgccgccgtt
     6421 gttgccgcgg ccgcagatga tcacgattcg acccggacgc ttccggttcc cggcgaggtc
     6481 ggcaagccat ttgtgaatca ggtccgccgc attgcgtccg gcgttttcca tgagcacgac
     6541 gcccggaatg ccgagctcct cgatggcgta cctgtctatg gcgcggcatt gggcacaggt
     6601 cagacgcagt tgatctgttg gttcgtccat ggccctattc tcgccgcagt gcggtgcgga
     6661 ctgcaactgg gagccgtgga cgggtttgct tgtgcttgaa tggacgccat gggccgatga
     6721 tcagaatgca cgggacgacc ggcgggcaag ccgccagcgg cgcccggtag cggcgatcag
     6781 ggtctgaggg cagcagagat actcagcgga ggccactatc gggaccccac tcgctccatt
     6841 tcaccacatg aatgggacgc cttgcgttag cggtctgatg gatcgttgcg ttcgtgtggg
     6901 aggaaagcta tcattacact tgcggaacaa gagagcctgc ctgggtaggt gggggtttcc
     6961 gcttgttttg aggagtgtcc cgacatgtca caagtatctc gccgatccct cctggccgca
     7021 tcggctgcgg cggccgttat cggagatttc ctgcccaaac gcgctgaagc agccgagagc
     7081 attccgggcg tcgaccagaa gaagaccgac cacgaccgga caaaagaatg gaagcccttc
     7141 agcgaccgca aagttcgggt cgggattgtc gggcatggcg tgtgtcagtt cggtctggcg
     7201 ttcgacctcc agaatcaccc gaatgtccaa ctggtggcgg tcagcgacct gtttccggac
     7261 cgctgtgcgg acatggctcg gaaagcgaaa tgcgagaaga cctacccgtc actggaagag
     7321 atggtcaagg acgattcgat cgaggccatt tattgcgcca ccgacgcacc gtcgcatgcc
     7381 aaacacgcca ttctgtgcat ggagcatggc aagcacgtgg ctaccgcggt tccggccttc
     7441 cggggcgaca tcgaagatgc cgaaaggctt ttggaatgtt gcaggaagaa caaggggttg
     7501 gtctatgcga tgttcgagac ttccgtcttt catgacgatc tgtatgccat ggagaagctc
     7561 tttgcggccg gggtgttcgg gcgcatcatc tacagcgaag gcgagtattg ccatcctcat
     7621 tctctcgacg ctccgccgct ggactcatac aaggattgga ggaaaaacgg ttgcccgatg
     7681 tggtatccga cgcatgcgac ggcctattat gtcggggtga cgcacaagag cttcgtcgag
     7741 gtttcatgtc agggcacgcc ggatttcgac gaatcccggc gggttcccaa cgcgatcgga
     7801 aacatcttca gatccgaagt cgggctgttt cggactgcgg agggcgggat ttcgcggatg
     7861 atagtctgct actcgcaagg cgagtacctg gaagcggggc gcatacgcgg cgaatatggc
     7921 ggattcaaca agacgttcgt cggggacgca acgggcaaga agcggtacga agaggctctt
     7981 gcgaagggct tgcagatcga gaagtacgcg ttgccgccgg gggtcgaacc gggcggccat
     8041 gggggctctc acggttacct tggcgacgac tttattgacg ccatccttcg ggggcgcaag
     8101 ccggcggtcg acgtgatcga cgcgctgaat atgaccgtgc cgggctacta tgcgcatcta
     8161 tcggcgttaa aggacgggga aacgttaaag attccgcagt acacgttata ggcgtgagga
     8221 tgggggcgag gtacaattgc ccatcgcggg cgccggcggg caacgtcatc cggaggaatc
     8281 tcagacagtt gccgactatg agcactgagc cacccagcaa aagtctggcg gcgatccagg
     8341 gcatcatcga tgccggacga ccgctgattt acatccagtc cgccgaagaa gaccgggtca
     8401 tcgggttgct ttcgcggatc gccgagcagc gacgcggggg ccgcgtggac ctgtttctct
     8461 ggagcgcgac ggaaggcctg caacagaatg gaaagccggt caagaaccag cctgatgggc
     8521 cgcgaggagt tctcgacttc gtcgtaacgc acgacaagcc cggccttttt ttgctcaagg
     8581 actttcacga gttcatgcgc gatgccgtcg agattcgccg tcggctccgc gacctttact
     8641 atcggtgtct gggcacggac aaattcgcgg tgatctgctc gccggtcaag tgcatcccgg
     8701 aggaaatcaa ccgcgaggtg gccttcattg aactgccgcg gccggacctg aaggaactgg
     8761 aggagctgct gcggaccgag gctgaatccg tccgtggggc cggcggctcg gcgggtctct
     8821 ccgcggagac catccaccgg ctcacgcgag cggtgcaggg gctgacgttt catgaggctc
     8881 ggcatgccct gcgacgggca atggccgagc atggcaagct cgatgaatcc atcttgcaga
     8941 cgctggaaca ggagaagcag caactcgtac gcaagacggg cctgatcgag tatgttcccg
     9001 gcaccgtcgg tgttgagcag atcggcgggc ttgacgttct caagacgtgg ctgatggagc
     9061 ggcgcgaact cttcttttct cgcgagagca tttcatcgga gatcgtgccg aaggggctcc
     9121 tgctcatggg ggtctcaggt tgcggcaaga gtctctcggc gcgggccgtg gcgaacgttt
     9181 tctgccttcc gctttatcgc atcgacatgg tccaggtttt ctctgccggg gtgggtaacg
     9241 cggagcggct tttcgccggc gcgtgccggg cgatggagga agtctcaccg gccgtggtgt
     9301 ggttcgacga gattgagaac gggatctccc gccaccagca ggatgaaacg ggcgcgctca
     9361 accggatttt tgggtttttt ctgacgtgga tgcaggaaaa gcccgccggg ctgttcgttg
     9421 cggcgacggc taatcggatc gaccttctgc cggccgagat gatccgcaag gggcggtttg
     9481 accaggtctt tttcattgac ctgccggact acgacgagcg gctggagatc ttcaagattc
     9541 atctctcccg tcgtggcgtg gacccgtcga cactgcgact cgatctgatc gccgagcgca
     9601 ccgagggctg gaccggcgcg gagattgagc aggcggtgat cgccgccctc gtttctgcgc
     9661 gtctggccaa ggaaaccgtc acggacgagc atctgttccc ggcgcttcgt cagatcgttc
     9721 cgctggccaa gacgatgagg gagcagatca accacattcg ttcctgggcc ttcgaccggg
     9781 ctgttcacgc ctcggcccgg aagaagtgag gggacgctgc cgtgtgagtt tggaactctc
     9841 caccggttcc tgggtggatt gcgcagtgag ccctgtcgga ccctccttct cttgtgcatc
     9901 tgcaccgaca agagcgcgta agcgggtgcg gcggagccct ctggcctggc aacttgatcc
     9961 gtaccctttc ggccaaggac gtgggcgggg tggggaggtt gggcgaggcg ttcggggtgg
    10021 atcggatgac ggggaatcgg gcacggtttt ccttaagtgt ttaatcgata acgacttaag
    10081 atctcggttg ggttcgtttg gcgcgggggg tggtcggcgg tcggtgtccg gcttttggcg
    10141 cgcgagggtc gcttcgggat ctgttg
//
LOCUS       DAOTPW010000212        10164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6179_length_10164_cov_5.405282, whole genome shotgun sequence.
ACCESSION   DAOTPW010000212 DAOTPW010000000
VERSION     DAOTPW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6179_length_10164_cov_5.405282"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1192)
                     /locus_tag="P5316_16750"
     CDS             complement(47..1192)
                     /locus_tag="P5316_16750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRR86663.1"
                     /translation="MARDGLPHSYSGTNRVNEYLQIGSQTLDYDADGNLSVDEEGRHY
                     DYDEYNRLVEVRAADDTTVLAHFTYDALGRRVASKIGQTERHYYYAGQTVIEEYNVNS
                     AGVESLARYHINGGQYIDERVVSYDVKSDGSAVPTYYLAGDNFSVIGTGSADGQIIRP
                     LNYSPTGQFSGSGIRSCNLDQDSTNDVDALDFALLEGCFSGPDVPVTTGPTCQIGEAA
                     DIDRDGDVDGADYIIFQTCMSGTDVPASAACLGQGVSPEDSGIAAMHGLTVDVLPDGK
                     ALLYARARHYDMKHGRWLQRDPKGYVDGPNLYEAFGGNALRFLDPFGQSANVLPIADP
                     CTAYKNITWLVDQFWKTVKSLPYFNQANEERLQRFGDSIAHLNLDDK"
     gene            complement(1782..2921)
                     /locus_tag="P5316_16755"
     CDS             complement(1782..2921)
                     /locus_tag="P5316_16755"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRR86664.1"
                     /translation="MLTRLMIWVPAAALLAGCAQQREVTFRMHTLNAESRFEAAGVCD
                     MNNDGRLDIISGGFWYEAPTWKKHFIRDIPEQDNYYYDFANIPVDVDGDGWIDHVTVA
                     WHNKQISWVRHPGNSTNPFEVIKIDDPGHFETAFGFDVNGDGQTDIVPNIFDGEPGWY
                     EFKPDPRMPHGVNWIKHPLPNDATGPGIGVGDINGDGRHDLVGGKGWAEQPADPKAEW
                     VWHAEFDLGDASIPILVHDIDGDGDLDLIWGIGHGYGLYWMEQGRNALGQRIWSKHEI
                     DKSWSQAHAPILADINNDRRMELIAGKRYYAHNGKDPGANDPRVIYWYDFDIARRQWK
                     RHVIAQGGPAGLGIAPAAADIDDDGDIDIVAPGKSGLYLFENLLR"
     gene            3074..4414
                     /locus_tag="P5316_16760"
     CDS             3074..4414
                     /locus_tag="P5316_16760"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRR86665.1"
                     /translation="MRHVRVSDVMLGKACLWLAAWFMVSAGCEQLPEPAKPGEPVKQQ
                     AKPGAQAGPGPQSQSRVTPAPPPRPVVPPLVDTWPPRGVWVPRDIYKSPRQIAALMEE
                     AKQAGLNTILFQVRGHAAAYYFSAIEPFAYEFPPGGPGFDPLAVACHEAHTRGLAIHA
                     WVNMMPAWAGDKPPDDPKHLYHTHPEWFLYDQKGKRQPLNGWYVSLNPCLPEVRNYLV
                     GICREIVTRYPVDGLHLDYIRFPTDRSPRGSDYPYDARTLELYRQATGLKPSSNRKTW
                     SQWRTYQVTRLVWEIHSMVKKERPKIRLTAACPPDLPTARNNYFQDGPLWLRSGYVDG
                     IFLMNYTSNNKTFRARHETWQREAGGRPVAAGIGIYMHGSDVTSLEQLKMARQWGGGF
                     AFFSSPYLFSGWPTSRQRLATLKPTLLEMKNAAMQSLPRRASPPTPGRAVPQVP"
     gene            complement(4482..5693)
                     /locus_tag="P5316_16765"
     CDS             complement(4482..5693)
                     /locus_tag="P5316_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR86666.1"
                     /translation="MKRFETTAFKGGIITMLRARIWSVLSLIVLSFLVLGQTGPCGSG
                     PVATGDRDHDGIEDAADNCPDDANTDQLDADDDGVGDACEDSSGSGGGDGGTDPWLDN
                     GTKVYPENKRRCGVWSGTIAMETHRIREWEWTCPEAYYSCYKVHSGKQDERTVVNATV
                     LLRDEALWHWYPVFEASGNLTYDEVNTDHVEAFPSKCLLLQDEIEELHDSWAIDHPQI
                     EIHASGWPEGFYPEYYYDFDAETLTFYPWRERDVDVTVTLIINPPNDGQQVHLTTKYT
                     GEYQCSGNGIPEDQDDLVPHPGNTFLELEGTYHWDPNGNDRLDIILSDDAPKIFDCTK
                     CCSAGSCADSQSTITSYEIHLTRFPEGDRDNDDVCDSIDNCPDTPNWDQLDSTGDGVG
                     DECDPLAPLVP"
     gene            6010..8736
                     /locus_tag="P5316_16770"
     CDS             6010..8736
                     /locus_tag="P5316_16770"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRR86667.1"
                     /translation="MKCSLLKSFQLLLLALITSAAAGEPIAPPEWAVRYTDGLKAWRD
                     IIYYWIDRQKPNGSFGFGWGEDEEMVVGWPGVMMAADDRRIQTALERMIENVWYNVNI
                     QNGYVWLATDAEHGPEPTSYGTPILLYQEFGSPKLIERLMVTASNVEYWTAHTPRGDR
                     HMRSTYFGSQQLYEWPFYDEDSPINARAWIPMMQLVLYNRDPHLLKYYLEWADAWAKH
                     AMEEVHGKPPGIMPGQVAFPTGEPGGHTQNWWGAGAHEFSSLWYQTRMHEVLAIAYAV
                     TGEDRYVRPIREMLRFFSTYARPAVKQDGPFPPDRLPAQYRFPPGWREGNESTRWARD
                     IALGHLGCWYYYLTGDTQFDGAFSSLLGERIERGPFSRIDRRIVDEGWRRAVSFRDKY
                     FTEVVPKLPYADSLANYAAAWWQKQYGTMTFGQRCFHDGGHLANSVNWPWVDFPPPSV
                     VWKDTGYETAVFVLEDAPQRLRVALCNLGEDGRQIGMQVFTLDDGIYNLRLGPDGDED
                     GEFDAVTTIQSVAVERGTRLRFALPNDVTTLLELVRRSDSGPVPAWGPRPDLGLDAWD
                     IMVSRSKPGPGESITFTIRVHNIGSRPAEKVKVRLLGRGGSEEALLGEALLDRIDAPQ
                     RLVPSFADVKVPWIVAEGVSTIGVCLDPEDAIDELYEGNNEAWAALADLATSQRPKEY
                     VAHAPATRPSLADVKADNLSRYDVPFRTGIRLDGVIDEREWRDVVEFAFIPHADKPLK
                     KRTWMRVAYDDQALYLALRCEEPDMQWLDTDLRRMEDIYYKDGFEIFLDPGAQVWRYW
                     QFCFDTVPHVFQTLSRNQFAKKAAWEVKVHKGGKDWSAEVRFPFSEFDVPPPKAGDRW
                     RMNAMRFTTTFRDPEHPEAKIAERSHFSPLGRLRGHHTPELFGDLYFRAKPE"
     gene            complement(8941..9519)
                     /locus_tag="P5316_16775"
     CDS             complement(8941..9519)
                     /locus_tag="P5316_16775"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRR86668.1"
                     /translation="MIVLIDNYDSFTYNLVQRIGELDRTREMKVYRNDKVTLDRIEAD
                     RPDHIIISPGPCTPKEAGISVDVVKHFAGKVPILGVCLGHQSIGYAFGGEIVRAKRLM
                     HGKTSEIQHDGKGLFVGLSNPFTATRYHSLVIKPDTLHSDFVVTARTTDAPDDEIMGI
                     RHKSMPIEGVQFHPESFLTLEGHKLLDNFLRM"
     gene            9706..>10164
                     /locus_tag="P5316_16780"
     CDS             9706..>10164
                     /locus_tag="P5316_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86669.1"
                     /translation="MRTTTAGISGPAILWVAVVGLLMAGCGGKTSGPRVTGTTKEATV
                     AKMEFSMSAERANDPRLEPLRYLEQYGKDHPPQLAFAAKDAAEWRRWRRELRKRLSDT
                     IGLPGMDDSGKPATEPRKLRVTAGPVAQCDGYKRMAFTIETGKGLYVPAFL"
BASE COUNT         1854 a         2937 c         3312 g         2061 t
ORIGIN      
        1 tgccatcctg cgtcaggttc ccgtcgtcgt aaataggtga ctgtccctat ttatcgtcca
       61 ggttgagatg cgcgattgag tcgccaaacc tctgcagccg ctcctcatta gcttgattga
      121 agtacggcaa ggacttcact gttttccaga attggtcaac aagccacgtg atattcttgt
      181 aggcagtgca ggggtcagca atagggagca cgttggcgga ctgcccaaac gggtcgagga
      241 agcggagcgc attgcccccg aaggcctcgt agagattcgg gccgtcgacg tagcctttgg
      301 ggtctcgttg gagccagcgg ccgtgtttca tgtcatagtg gcgggcgcgg gcgtagagga
      361 gggccttgcc gtcggggagg acgtcgacgg tcaggccgtg catggcggcg atgccggagt
      421 cttctgggga cacgccctgg ccgaggcagg cggcgctggc gggcacgtcg gtgccgctca
      481 tgcaggtctg gaagatgatg tagtcggccc catcgacgtc gccgtcgcgg tcgatgtcgg
      541 cggcctcgcc gatctggcag gttgggccgg tcgtgacggg cacgtccggg ccgctgaagc
      601 agccttccag aagggcaaaa tcgagggcgt cgacgtcgtt ggtcgaatcc tggtccagat
      661 tgcaggatcg tatgccgctg ccggagaact ggccggtggg cgagtagttc agcgggcgga
      721 tgatctggcc gtcggcgctg ccggtgccga tgaccgagaa gttgtcgccg gccaggtagt
      781 aggtgggaac ggccgagccg tcggatttga cgtcatagct gaccacccgc tcgtcgatgt
      841 actggccgcc gttgatgtgg tagcgggcca gggactcgac gccggcactg ttgacgttgt
      901 attcctcgat gaccgtctgg ccggcgtagt agtagtggcg ttcggtctgg ccgatcttgg
      961 aggccacccg ccggccgagg gcgtcgtaag tgaagtgggc caaaacagtt gtgtcatcgg
     1021 cggcccggac ctcgaccagc cgattgtatt cgtcgtagtc gtagtgccgc ccttcctcgt
     1081 cgaccgacag gttgccgtcg gcatcgtaat cgagggtctg cgagccgatc tgcaggtact
     1141 cgttgacccg attggtgcca ctgtaggagt ggggcaagcc gtcgcgagcc accgagctct
     1201 cgcggttgcc aacgggcgaa aggtcgcagg ctgaagaaac tgtttaccct actgaaaacc
     1261 tctagaagcg ggggaacaag acacgcccgg gcaggcctgc gccgggccac gggcgtcaca
     1321 gaacccctga aaatgcgagg ttccgccccc gttgagggta aacagctaca ggctgaatct
     1381 tgagagaatc atcctgacac gttttctttt cttgagaaaa tcatcctgac acgttttctt
     1441 ttcttggtag cgtcgctggt taggcacgct gaatcctcaa agatagcttt gcggcgagag
     1501 atagaaccgt catccctggc aatcggccgg aagatcgggc gccctttcca tgagaaccgt
     1561 gggcgcgcag ccgcaaacag caatgagtgc gcttgagaat gctattcaag ctatcttgtc
     1621 catgtcagga ttggacacag aaccccgcat gaggctggag ggaagggggt agcacgtcac
     1681 ggcgcaccga attctgaatc gtcaagggcc gacgagggcg tcggccccag tgaacctcgc
     1741 cgcggcgagt cgcttacggc gacctgaacc ctcgatcctt cctacctgag caggttctcg
     1801 aacaggtaca gcccgctttt tccgggggcg acgatgtcga tgtcgccgtc gtcgtcgata
     1861 tcggccgcag cgggggcgat gcccagaccg gcggggccgc cttgggcgat gacgtgtcgc
     1921 ttccactgtc tcctggcgat gtcaaagtcg taccagtaga tcactctcgg atcattggct
     1981 ccggggtcct tgccgttgtg ggcgtagtag cgcttgccgg cgatcagctc catccggcgg
     2041 tcgttgttga tgtcggccag gatcggagcg tgggcctgcg accagctctt gtcgatctcg
     2101 tgtttggacc agatgcgctg gccgagggcg ttacgacctt gctccatcca gtataacccg
     2161 taaccgtgtc cgatgcccca gatcagatcg agatcgccgt ctccgtcgat gtcatgcacg
     2221 agaatcggga tactcgcgtc ccccaggtcg aactcggcat gccagaccca ttcggccttg
     2281 gggtcggccg gctgttcggc ccagcccttg ccgccaacca agtcgtggcg gccgtcgccg
     2341 ttgatgtcac cgactccgat gcccggaccg gtggcatcat tgggcagcgg gtgtttgatc
     2401 cagttgacgc cgtggggcat ccgcgggtcc ggcttgaact cgtaccatcc gggctcgccg
     2461 tcgaagatgt tgggtacaat gtccgtttgc ccgtcgccat tgacgtcgaa accgaaagcg
     2521 gtctcgaagt ggccggggtc gtcgatcttg atcacctcga acggattggt gctgttgccc
     2581 ggatgtcgaa cccatgagat ctgcttgttg tgccaagcga cggtgacgtg gtcgatccaa
     2641 ccgtcgccat ccacgtcgac cgggatgttg gcgaagtcgt agtagtaatt gtcttgctca
     2701 gggatgtcgc ggatgaaatg ctttttccac gtcggggctt cgtaccagaa gccgccgctg
     2761 atgatatcga gcctgccatc gttgttcata tcgcagaccc cggcggcctc gaagcgggac
     2821 tcggcgttga gtgtgtgcat cctgaaggtc acctcccgct gctgcgcaca gccggccagg
     2881 agggccgcgg cgggaaccca gatcatcagg cgggtcaaca tcactgtgct cctttctgaa
     2941 gacgagattc acccatctga gccggacaag gcggcaaagt caatgacagt tgttttcggt
     3001 cggcgcaagc gatttcgggc ttttcgcctg cgcgatcatc gttatgatag ggtcattcag
     3061 cgagggcggt tcgatgcggc atgttcgggt aagcgacgtg atgttgggga aggcctgtct
     3121 ttggctcgcg gcatggttca tggtcagcgc gggctgcgag caattgccgg agcccgccaa
     3181 gcccggcgag ccggtgaagc agcaggccaa gcctggggcg caggccgggc ccggcccaca
     3241 atcgcagtct cgcgttacgc cggcacctcc gccccggccg gtcgtgccgc cgttggtcga
     3301 cacgtggcct ccgaggggag tgtgggtgcc gcgggacatc tacaagtcac cccgccagat
     3361 cgccgcgctg atggaggagg ccaagcaggc gggcctgaac accatactgt tccaggtgcg
     3421 aggccacgcc gcggcttact atttctccgc catcgagccg ttcgcatacg agtttccgcc
     3481 cggcggaccg ggttttgacc cgttggcagt ggcttgccac gaagcccaca cccgcggtct
     3541 ggccatccat gcatgggtca acatgatgcc tgcgtgggcc ggcgacaagc ctccggacga
     3601 ccccaaacac ctttaccata cgcaccccga gtggttcttg tatgaccaga agggcaagcg
     3661 gcagccgctg aacggctggt acgtgagctt gaatccttgt cttccggagg tgcgcaatta
     3721 cctggtgggc atctgtcgcg aaatcgtgac gcgctatcct gttgacggct tgcatctcga
     3781 ttacattcgc tttccgacgg atcggtcgcc ccggggcagc gactatcctt acgatgcccg
     3841 cacgctggag ctttatcggc aggcgaccgg cctcaaaccg tcgagcaacc gcaagacctg
     3901 gtcgcaatgg cggacatacc aggtgacgcg tctggtgtgg gagatccaca gcatggtgaa
     3961 aaaagagcgg ccgaagattc gcctgactgc ggcatgtcca ccggacctcc ccacggccag
     4021 gaacaactac tttcaggacg gaccgttatg gctgcgatcg ggctatgtgg acggcatctt
     4081 cctgatgaat tacacctcga acaacaagac ttttcgtgcg aggcacgaga cctggcagcg
     4141 cgaggccggc ggccgaccgg tggccgcggg aatcgggatc tacatgcacg gatccgatgt
     4201 cacctcgttg gagcaactga aaatggcccg acagtggggc ggcggtttcg cgttcttctc
     4261 ctcgccttac ctgttcagcg gatggcccac gagccggcag cggcttgcga cgctcaagcc
     4321 gacgttgctg gagatgaaga atgcggccat gcagagtctc ccgcggcggg cttcgccgcc
     4381 cacccccggc agggccgtac ctcaagtgcc gtgagaatct cttgccgggg gctgagggaa
     4441 aagctcgcag gccgccctct ttccggtcgg ggcgttgcga ctcagggaac cagtggcgcc
     4501 aggggatcgc actcatcgcc gacgccatcg ccggtggagt caagctggtc ccagttcggc
     4561 gtgtccgggc agttgtcgat gctgtcgcag acgtcgtcgt tgtcgcggtc gccctcaggg
     4621 aaacgcgtca agtggatctc gtaggaggta atcgtgctct gggagtcggc gcacgaaccg
     4681 gcggagcagc acttggtgca gtcgaagatc ttgggtgcgt cgtccgacag aatgatgtcc
     4741 agtcggtcgt tcccgttcgg gtcccagtgg taggttccct ccaattcaag gaaggtattc
     4801 ccgggatggg gcacaaggtc atcctggtct tccgggatgc cgttgccgga acactgatat
     4861 tcgcccgtat acttggtggt caggtgtacc tgctgcccgt cgttcggagg gttgatgatg
     4921 agcgtgaccg tgacgtcaac gtcgcgctcg cgccacgggt agaaggtcag ggtctcggcg
     4981 tcgaaatcgt agtagtattc agggtagaat ccctccggcc atccggacgc gtggatttcg
     5041 atctggggat ggtcaatggc ccaggaatcg tgcagttcct cgatctcgtc ctggaggagc
     5101 aggcacttgg acgggaaggc ctcgacatgg tcagtgttta cctcgtcgta ggtcaggttc
     5161 ccggaggcct cgaacaccgg gtaccagtgc caaagcgcct catcccgcag caggacggtg
     5221 gcattgacga cggtccgctc atcctgtttg ccgctgtgaa ccttgtagca actatagtat
     5281 gcctcggggc aagtccattc ccactcacgg atacggtgcg tttccatggc gatggtgccc
     5341 gaccacactc cgcatcgccg cttgttttcg ggatagacct tggtgccgtt gtccagccac
     5401 ggatccgttc ctccgtcccc gccgccactg cccgacgagt cttcacacgc gtccccgacg
     5461 ccatcgtcat cggcgtcgag ttggtcggtg ttggcgtcgt cagggcaatt gtcggccgcg
     5521 tcctcgatac cgtcatgatc gcgatctccc gtggcgacgg gtccggaccc acacggcccc
     5581 gtttgaccca ggaccagaaa actcaggacg ataagcgaca aaacactcca gattcttgca
     5641 cgcagcattg tgatgatgcc ccccttgaat gcggttgtct cgaatcgctt cacaggtgtc
     5701 tttccaggac acgatgccgc cagtgtacgg catacggtcc agctgtgtcc aatctctcat
     5761 ctgatttgct cagcgtgcag gaaacaaagc cgcactgttt agtccgttcc gtcgcgactg
     5821 gtgtgcattt tgcgcgcatg tttccggatc gcggtgcgct tgggttgcgg cccggaaaaa
     5881 ggcggtccgc cttaggtcct ggttcgggtc ttgctcgtgc tgaggcacga tctcgattat
     5941 cttgagtcgg gtcttggtgg tcttgcggcc ggccaggggt gtggcccgac ccacgtcacg
     6001 aggtgcccga tgaaatgctc attgctgaaa tcgttccaat tgctcctatt ggctctgatc
     6061 acgagcgcgg cagccggaga acccattgct cctcctgagt gggccgtccg ttataccgac
     6121 ggactcaagg cctggcggga catcatctat tactggattg atcgccagaa gcccaacggc
     6181 agcttcggct tcggttgggg cgaggatgag gagatggtgg tcggttggcc gggtgtcatg
     6241 atggccgccg acgaccggcg tatccagacc gcccttgagc ggatgatcga gaatgtctgg
     6301 tataacgtga acatccagaa cggctacgtg tggcttgcca ccgatgccga gcacggtcct
     6361 gaacccacct cttacggcac acccattctg ctgtaccagg agttcggcag ccccaagctt
     6421 atcgagcgcc tcatggtcac ggccagcaac gttgagtact ggacggccca cacgccgcga
     6481 ggcgaccggc acatgcgctc gacgtacttc ggctcgcagc aactgtatga atggccgttc
     6541 tacgacgaag attcccccat caacgcccgg gcgtggatcc cgatgatgca actcgtactc
     6601 tacaaccggg acccgcattt gctgaagtat tacctcgagt gggctgacgc ctgggcgaaa
     6661 cacgccatgg aagaggtcca cggcaagccg ccgggcatca tgcccgggca ggtcgcattc
     6721 ccgacgggcg agccgggcgg acacacccag aactggtggg gggccggggc acacgagttc
     6781 agcagtctgt ggtatcagac ccgcatgcat gaggtgctgg caatcgccta cgcggtcacc
     6841 ggcgaagatc gctatgttcg tccgatacgg gagatgcttc ggttcttttc aacctatgcc
     6901 cggccggcgg tgaagcaaga cgggccgttc ccgcccgatc gcctgcctgc tcagtaccgg
     6961 tttccgccgg gttggcgtga gggcaacgaa agcacgcggt gggctcgcga tatcgctttg
     7021 gggcacctgg gttgttggta ttactatctg accggcgata cgcagtttga cggcgccttc
     7081 agcagcttgc ttggcgaaag gattgaacgc gggccttttt cgcggatcga ccggcggatt
     7141 gttgatgagg gctggcggcg ggcagtgagc tttcgcgaca agtatttcac ggaagtggtg
     7201 cccaagttgc cgtatgcgga ctcgctggcc aattacgccg ccgcctggtg gcagaagcag
     7261 tacggaacga tgaccttcgg ccagcggtgc ttccacgacg gcgggcacct ggccaacagc
     7321 gtcaactggc cctgggtgga cttcccgcct ccttccgtgg tctggaagga taccggctat
     7381 gagacggccg tcttcgtgct ggaggatgcg ccgcagcgcc tgcgggtggc gttgtgcaat
     7441 ctgggtgaag acggccggca gatcggcatg caggtattca cgctcgacga tggcatctac
     7501 aacctgagac tcggcccgga tggtgatgaa gacggcgaat tcgacgcggt gaccacgatt
     7561 cagtcggtag cagtggaacg gggcacgagg ctgcggtttg ccttgccgaa cgatgtcacc
     7621 acgctgctgg agctggttcg tagatcagat agcggaccgg tgccggcgtg gggcccgcgg
     7681 ccggacctgg gcctcgacgc ctgggacatc atggtctccc ggagcaagcc cggcccgggc
     7741 gaatcgatca cgtttacgat ccgcgtgcac aacattggca gccggccggc ggagaaggtg
     7801 aaggtgcgac tccttggacg cggcggcagc gaggaggctt tgcttggtga agccttgctc
     7861 gatcgaatcg acgcgccgca gcgactcgtt ccgagtttcg ccgacgtgaa ggtgccgtgg
     7921 attgtggccg agggtgtatc aacgatcgga gtctgcctcg acccggagga cgcgattgac
     7981 gagctttacg aggggaacaa cgaggcctgg gcggcacttg ccgatctggc cacatctcaa
     8041 cggccgaagg agtatgtggc tcacgcgcct gccactcgtc caagcctggc ggacgtgaag
     8101 gccgacaacc tgagccgtta tgacgtgccg tttcgcaccg gaatccggct ggacggcgtg
     8161 atcgatgagc gggaatggcg ggatgttgtg gagttcgcgt tcatcccgca cgccgacaag
     8221 ccgctcaaga agcgaacctg gatgcgcgtg gcctatgacg accaggctct gtatctggcg
     8281 ctgcggtgcg aggagcccga catgcagtgg ctcgacaccg acctgcggcg catggaggac
     8341 atctactaca aagacggttt cgagattttc ctcgaccctg gagcgcaggt gtggcgatac
     8401 tggcagtttt gtttcgacac cgtgccgcac gtgtttcaga cgctttcgcg caaccagttc
     8461 gcgaagaagg ccgcgtggga ggtgaaagtc cacaagggcg ggaaggactg gtcggcagag
     8521 gtacgatttc ccttcagcga attcgatgtg ccgcccccca aggccggtga ccgctggcgg
     8581 atgaacgcca tgcgtttcac cacgaccttt cgtgatccgg agcacccgga ggcgaagatc
     8641 gccgagagat cacacttttc gcccctcggc aggctacgcg gccaccatac gccagagtta
     8701 tttggagatc tctactttcg cgcaaaaccg gagtgatgag aaccgaatga cggaagaaga
     8761 cagaaagaag tggtcagtga tcagttgtca gtggtcagtc attggccaag gttgaaactg
     8821 agaacggatc actgatgact gagaacttgg cttcgtcatt cggatttcga cattcgtcat
     8881 ttcgtcattt gcatgcgagg cggctccccg gcaagatccg tgcatggggc tcggaaggtc
     8941 ctacattcgc aggaagttgt ccaggagctt gtggccttcg agggtcagga agctctccgg
     9001 atggaactgg acgccctcga tgggcatgga cttgtggcgt atgcccatga tttcgtcgtc
     9061 cggcgcgtcg gtcgtgcggg cggtgaccac gaaatccgaa tgcagagtgt cgggtttgat
     9121 gaccagcgag tggtaccgtg ttgcagtgaa cggattgctc aagccgacga acaagccctt
     9181 gccgtcgtgt tggatctcgc tggttttgcc gtgcatgagc cgcttggcgc ggacgatctc
     9241 gccgccgaag gcgtatccga tcgactggtg gccgaggcac acgccgagga tcggcacctt
     9301 tccggcgaag tgtttgacca cgtccacgct gatgcccgct tcctttggag tgcaggggcc
     9361 gggcgaaatg atgatgtggt cgggtctgtc ggcttcgatc cggtcgagcg tgaccttatc
     9421 gttgcggtag actttcatct cgcgcgtgcg gtcgagttct ccgatccgct gcacgaggtt
     9481 gtaggtgaac gagtcgtagt tgtcgatcag cacgatcatg ctattcagcg taggtgtcgg
     9541 ggcgagcatt gtcaacgcgg tcttgccggc tacactggtg ccatcgttgc aggaccggtc
     9601 tttgaccgcg atgcttgacg agccgagggg cttgtccctt cgctattgat cgaatccgga
     9661 aagaccggca ggatcaacct gccgggttat caggagagtc cgagaatgcg cacaacgact
     9721 gcgggtatca gtggtccagc gattctgtgg gtggcggttg tcgggttgct gatggccggc
     9781 tgcggcggca agacctcagg tccgcgggtg acgggtacaa cgaaggaggc gactgtggcc
     9841 aagatggagt tctcgatgtc ggccgagcgg gccaacgacc cgcgactcga gccgctgcgt
     9901 tacctcgaac aatacgggaa agaccacccg ccgcaattgg cgttcgccgc caaagacgcc
     9961 gccgaatgga ggcgatggcg gcgtgagctt cgcaagcggc tgtcggacac catcggtttg
    10021 ccgggaatgg acgattcggg caagccggcg accgagccgc gcaagctgcg ggtgaccgcc
    10081 ggcccggtag ctcagtgtga cggctacaag cgcatggcgt tcaccattga gacgggcaag
    10141 ggcttgtacg tccccgcatt cctt
//
LOCUS       DAOTPW010000213        10159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6184_length_10159_cov_5.850752, whole genome shotgun sequence.
ACCESSION   DAOTPW010000213 DAOTPW010000000
VERSION     DAOTPW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6184_length_10159_cov_5.850752"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2306)
                     /locus_tag="P5316_16785"
     rRNA            complement(<1..2306)
                     /locus_tag="P5316_16785"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(2830..2901)
                     /locus_tag="P5316_16790"
     tRNA            complement(2830..2901)
                     /locus_tag="P5316_16790"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2867..2869),aa:Ala,seq:tgc)
     gene            complement(3055..3128)
                     /locus_tag="P5316_16795"
     tRNA            complement(3055..3128)
                     /locus_tag="P5316_16795"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3092..3094),aa:Ile,seq:gat)
     gene            complement(3598..4302)
                     /locus_tag="P5316_16800"
     CDS             complement(3598..4302)
                     /locus_tag="P5316_16800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRR86670.1"
                     /translation="MARIAVLQHFWCENAGVFEPALQRLGHSVENVELFNGSAVPGKR
                     DFDAWIVMGGPMNVDETDRYAFLGPERALLADLIAEDRPVLGICLGSQLIARAAGAKV
                     YPKRPKEIGLFEISLLPAAATDPLLCLFDDPQEVFQWHGDTFDLPARAVHLARSSRFE
                     NQAFRLGRRVYAFQFHLESTGKMIDDLSRACARELMELPAGEGFEQYRPRFQQALAAQ
                     NRLADKVIESWAGLFD"
     gene            4518..5462
                     /gene="lepB"
                     /locus_tag="P5316_16805"
     CDS             4518..5462
                     /gene="lepB"
                     /locus_tag="P5316_16805"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRR86671.1"
                     /translation="MPPDEPAATAHEPAVLDYAALDKVPDGWGKGFMAVAASILLPGL
                     GRLFAGRRRRGVLWLVVCLAIGLAGAVCLAYPAALPIAAGLAAIETVLVLLMLIDAFI
                     GGRRSDRRMLGRPWLRYLVGIGFLLATAFASPRFLLAYHLRDHYVEAFVTSSGAMSPT
                     LMPGDKFLAHKGVDVHRHSMVVFEHPQFGPGVKIVNRIVGLPGETVEIVDGQLQIDGQ
                     SISPPPGVTGYTSMLYMGVDDRSLRGKPGAGCEGNPIHLGPDEYYVLGDNTTRAKDSR
                     LWEVVAEGHQLGVLPGDHIIGRVMAIYWPPQRWRRFQE"
     gene            5642..6277
                     /locus_tag="P5316_16810"
     CDS             5642..6277
                     /locus_tag="P5316_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86672.1"
                     /translation="MTRQQMKLIVIGASTLLTAGTALAHVPYLETTDFTWEKPFRVRG
                     SIAQSIAVYAWLEMADGATEDIDVYQFNLRRSTQVFVETLVPICPAYAEFRPSFAIVG
                     PGLDVPAESLPFELPVGYGAIVVPNYSDPQRPTFYEPFGNKSYYDGPEFKQVLSTPGT
                     YYVVVWDPAGIGGDYVAVLGDKEIWWPWDIIRALIQTPKIRRGLELHTDCR"
     gene            6588..7160
                     /locus_tag="P5316_16815"
     CDS             6588..7160
                     /locus_tag="P5316_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86673.1"
                     /translation="MKPHSGKRAPIKWYGVLLQICFAPVAVLITFAAILTAPVLVFKM
                     ITEERKQREKVRQFLRGQGARALLVWHSRRGWNEFGEKNLLPALPEGIVAVHDRRRRD
                     STYRDRRSAEQADGVSELARPYLLFFHKARLQAISLNRALWPLKRQRKPSLAVQREVS
                     RIILDHVADSPIRLDSAPRDGSTDMLPMRN"
     gene            7126..10101
                     /gene="mutS"
                     /locus_tag="P5316_16820"
     CDS             7126..10101
                     /gene="mutS"
                     /locus_tag="P5316_16820"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRR86674.1"
                     /translation="MAQLTCFQCVIENPVALPYISAAMTREGTKPKCEPPAEGLTPAM
                     RQYIEQKAAAPDALLLFRMGDFYETFYDDAITAARVLGITLTTRGKDRNGQAVPLAGI
                     PYHALEGYLTKLVKAGFKVAISEQLEDPKLAKGVVKRDIVRIVTPGTLTDDALLERKA
                     DNYLVCLHHRRDETGLACVELSTGAFWVQTVAAKEILNELVRLSPAELLLAEVAIDVR
                     DPLAHVVKELTGPNPIIPSLLVTRRPAHVFDPYQADQRLRRHFGVATLEGFGFDRIDA
                     SLSAAAAIIDYLSETQKGSLNHIVKLTRRETNDFVLIDQATWRSLEIERTIRGGSTSG
                     SLLAAVDRTSTPMGARCLRRWLAAPLRDAGRIRERQRAIADLLTDRHLLQTLRTEMGR
                     LADIERITARLGVGRASPRDLLALGQTLLGVERIAATLDRLAAQTSITGDRLCAFLAE
                     RRDALRGEHDLAEFLTTALRPNPPQVLNEGGIIADGYDAELDRLRTMSSNAQQWLADY
                     QAREIQRTGISSLKVGYNQVFGYYIEITHAHASRIPPDYVRKQTLKNAERYITDELKQ
                     YETEILTARDRAIAREQELFEVIRRRAVERIAPLQQLAAAISEIDVVAGLAHLADERR
                     YVCPEMVEGNALEICEGRHPVLEQTLAEKFVPNDCRLGAVSDQQSAVSGRRSAVSQTD
                     CATSGQQSAVSQTDSAAIRPDSVGLFGPQADLKSEISDRQSDISDLKSQISNSKSETL
                     AILTGPNMAGKSTYIRQIALLTILAQTGSYVPAASMRFTPADRIFARIGASDELTRGQ
                     STFMVEMTEAANILNNASEASLVIIDELGRGTSTFDGLALAWAIAEQLVVGVGCRTLF
                     ATHYHELTELEKYMNGAVNYNVAVREWQDEVVFLHRIVRGKTDRSYGVHVAKLAGVGP
                     EVIARSREILAELEANFSATRRAPRRTARRNRRDDQLLLFADPADEVIKEIRQLDPHK
                     LTPIDALQAIEKWRKRLEE"
BASE COUNT         1957 a         3275 c         2880 g         2047 t
ORIGIN      
        1 gttaggatac aaatagcaca agggtggtat ttcaacgttg gctccccaac cgccggaacg
       61 gctggatcat cgcctcccac ctatcctacg catgtgatat ctgtggccaa tatcaggctg
      121 cagtaaaggt tcacggggtc tttccgtctt gccgcgggta tgtggtatct tcaccacaat
      181 ttcaatttca ccgagtcggt ccttgagaca gtgctccagt cgttacgcca ttcatgcgcg
      241 tcggaactta cccgacaagg aacttcgcta ccttaggacc ctcatagtta gggccgccgt
      301 ttacgggtgc ttcggtcgcc agcttctcag gattgctccc aatgaccggc ttccttaaca
      361 ttcccgcacc gagcaggcgt cagtctctat acgttgtctg tgggacttag cagagacctg
      421 tgtttttgat aaacagtcgc cagagccgct ttgctgtgcc ctctccaaac tattgctaat
      481 ctggagtagg aacccctttt cccgaagtta cggggccaaa ttgcctagtt ccttaaggac
      541 cgttctctcg agcgcctgag gctacgcgcc tcacctacct gtgtcagttt tagtacggac
      601 gtgctgctcg tcctcgcgtg cttttcttgg ttctccctcc ccacacttcg gcaacgaatc
      661 ggcctcgccc tttcgggaac cctcacgtct agtggtgggc tgcagcttgg gaaaacgcac
      721 acgcgactca acctcacaac acggggcagg aatattaacc tgctgtccat cggctacgcc
      781 tttcggcctg gccttaggaa ccgcctaacc ctgggcggat tgcccttccc caggaaacct
      841 taggttttcg gcgagcgggg tttacacccg ctttctcgtt actcataccg gcataatcac
      901 ttggtaggac cgcaaccact cctcacggta tggcctgtat cttcctacca acgctcctct
      961 actactcgag aggagctgtc agcactcagc actcgacagt cagccgcaaa aagcgcccgt
     1021 ttggaacgca ctgatgggct gaaggtcgat ggctgaaggc tgatagcccc tctcgagtcc
     1081 gctgcttcgg tatcatgctt agtcccgttc attatcggcg cccaacctct cgacgggtaa
     1141 gctgttacgc actttttaaa tggtggctgc ttctaagcca acatccccgc tgtctcggag
     1201 attgaacttc cttaagcact gagcatgaat tggggacctt agcaggcggg ctgggttgtt
     1261 tccctttcga ccatgaatgt tatcacccac agtctcactc ccgggatagt cgttgcggta
     1321 ttcggagttt gattgaaggc ggcaagctgg tggctcccgc gtttcattca gtagctctac
     1381 ccccgcaacg tagtggcccg aggctgcccc taaaggcatt tcgaggagaa ccagatatct
     1441 ccaggtttga ttagactttt actcctcccc acagctcatg ccagaacttt tcaacgttca
     1501 cggcttcgga cctccgcgtc cttttacggg cgcttcatcc tggccatggg tagatcacct
     1561 ggtttcgggt ctacccccaa cgacattgtc gccctgttca gactcgcttt cgctatggct
     1621 ccgcaccgga ggtgcttaac cgcgccgctg aggataactc gccggtccat tatgcaaaag
     1681 gtacgccgtc acccggaccc ccgaaagggt atcgggctcc gaccgctggt aagtgcatgg
     1741 tttcaggttc ttttgactcc ccttataggg gttcttttca cctttcggtc accctacttg
     1801 ttgactatcg gtcgtcgagg agtacttagc cttggagggt ggtcccccca gcttcacacg
     1861 gagttccacg agctccatgc tactctggaa agccgatggt aacgcggggc tgtcgttgtc
     1921 gcatacaggg ctgtcacctt ctgtggcacc catttccatg agtttctgct aacgaagcct
     1981 ttgtaaccgc tctcggctcc cgcaaccccg atccgaagac cggtttgggc tatgtccgcg
     2041 ttcgctcgcc gctactgacg gagtctcggt tgatttcctt tcctccaggt actgagatgt
     2101 gtcagttccc tgggttcgct cgcacgggtc tatgcattca acccgccgtg acgccatctt
     2161 cttgcgaagt gacgccgggt ttccccattc ggacatcccc ggatcaaagc tcgtttgcca
     2221 actccccggg gcttatcgca ggctaccacg tccttcatcg cctctcgacg ccaaggcatc
     2281 caccatgcac ccttactagc ttgatcgtat gaatgacggg ctgttgcccg gcactcaccc
     2341 atcaagacac cttcgacgca tcggttgcgt ctttgaaacg agaaacagtc tctcgtcgct
     2401 cgaagattga actggtgatg tgaatcgcca ctacctgcgc atctgcccgg cgccgcccct
     2461 cggcggccgc cgccagatct gcacacctat ccgattgtca aagaactcgc ttcggagcct
     2521 tgccgagccc ggctcctgac gccttgacgg tttccggcgc caggcttgct caaccccgga
     2581 gccctgtaag tatatcgcca atcccgcacg tgtaaagacc acctcgcggg aattcttcac
     2641 ttttcctgtg gccggcaaac catttctcgg ccgatccggg ccgttttcat cggcctgcgg
     2701 cgaacggccg ccccacgggc ttgccacatc gtcgttcgcc gtcttctatc cgacggcccg
     2761 actaccccgc cttcgcggct agacccgcca cgccgtggca gtcttcattc gcctgcggcg
     2821 aacgaagact ggagccgacc ggggtcgaac cggcaacctc ctggttgcaa accaggcgct
     2881 ctcccattga gctacggccc ctgaaaaact gtcagcattc agccgtcagc tttcagtcat
     2941 acaggcatct gcgatgccgc tggccgaagc ccaacacatc tcaccccagc catcagtctc
     3001 cagccttgcg cctatctggc tgaccgctga aggctgaaag ctgacagctc ctcatgggcc
     3061 cgagaggatt cgaacctctg acctcgtcct tatcagggac gcgctctaac caactgagct
     3121 acgagcccgc aaaacgtgcc attctggtta tcactccgcg tcccgcaaaa cgctgcctgc
     3181 ctgggacgtt ttcctggcca catcctgcgg actcgcccgc attgatgcct ctcggttgtc
     3241 ggttccctca aaaatgggcg acaaaaaaac cgccagctcg ctgctgacgg ctcgtcatca
     3301 ccgcctgacg ataccgactg cggccagcta ggtcacccct ccattacgct catcgcgttt
     3361 gtattgtttg cgcaaaaggc cttttccctt tctacacggg ggcccgcctc aatagctgtc
     3421 cacaacgaac agctatcggc tctttcccgt gaattagtgt acaccgtctc gccaggttgt
     3481 caactctggc cccgccccgc ccgcgacgag ttgagccaac cagcccgccc gaaggctgtc
     3541 tgatgaacac aaacccttga tgtacataag gttataccat gaatgggtgc cggcttgtca
     3601 atcgaaaagg ccagcccagc tttcgatgac cttgtcggcc agccggttct gggcggccag
     3661 agcctgctgg aacctcggcc ggtattgttc gaaaccctcg cctgccggaa gctccatgag
     3721 ctctctggca caggctcggc tgagatcgtc gatcatcttc ccggtcgatt ccaagtgaaa
     3781 ctgaaaggca tagacacgcc gcccaagccg aaacgcctgg ttctcgaaac gggacgatcg
     3841 agccagatga acggcacgcg caggcagatc aaacgtgtca ccgtgccact ggaacacttc
     3901 ctggggatcg tcaaataagc acagaagagg atcggtggcc gccgcaggca acaggctgat
     3961 ctcgaagagg ccgatttcct tcggtcgttt gggatacacc ttcgcgccag ccgctctggc
     4021 gatgagttgc gaacctaggc agatccccag aacgggtcga tcctcggcga tcagatccgc
     4081 aagcaaagcc cgctccggac cgagaaaagc gtaccggtcc gtctcatcca cgttcatcgg
     4141 gccgcccatg acgatccaag cgtcgaaatc acgcttgccg ggcaccgcac taccgttgaa
     4201 caactcgacg ttttcgacgc tgtgaccaag ccgttgaagc gccggctcaa acacacccgc
     4261 attctcgcac cagaagtgtt gcagcaccgc gatccgggcc atgtgggctc cctcacaatc
     4321 attagcacgg caaatagtat ctcatgcgga tcataagcca tctgcagaga gctcggcaag
     4381 cggtcaggca cgcgacctga gtcgtcattg ggaagcccat acgccgtgcc ggttgggcgc
     4441 tttgcaggtt gcccccagaa gtataaggta aacaagggtc ggttgttcag agccgcaggg
     4501 aattatagga gacccccatg ccaccggatg aacccgccgc aacagcacac gaacctgccg
     4561 tgctggacta cgcagcgctc gacaaagttc cagacggttg gggcaagggc ttcatggcgg
     4621 ttgctgcatc tattctgctg cccggcctgg gccggctctt cgccgggcgg cgccggcggg
     4681 gggtgttgtg gcttgtggtc tgcctcgcga tcggcttggc gggcgcggtc tgtctggcgt
     4741 accctgccgc attgccgatc gcggccggcc tggccgccat cgaaaccgtc ctggtgctcc
     4801 tgatgctcat cgacgccttc atcggggggc gccgatcgga ccgtcgaatg ctcggacggc
     4861 cgtggctgcg gtaccttgtc gggatcggtt ttcttctggc gacggctttt gctagcccac
     4921 gattcctgct cgcataccat cttcgagacc actacgttga ggctttcgtc accagcagcg
     4981 gcgccatgtc gccgacgctg atgccaggcg acaagtttct ggcccacaag ggagttgacg
     5041 tacaccgtca cagcatggtg gtgtttgagc accctcagtt cggtcccggc gtgaaaatcg
     5101 taaatcggat cgtcggcttg cccggcgaga ccgtcgaaat cgtcgacgga cagctccaga
     5161 tcgacggtca gtcgatatcg ccgccgcccg gcgtgaccgg ctataccagc atgctctata
     5221 tgggtgttga tgatcggtcg ttgcgaggaa aacccggcgc cggatgtgag ggcaacccga
     5281 tccatctcgg cccggatgag tactacgttc tcggcgacaa caccaccagg gcaaaggact
     5341 cccggctctg ggaggttgtg gccgaaggtc atcaactggg agtcctgcct ggcgatcata
     5401 tcatcggccg ggtcatggcc atctactggc cgcctcaacg ctggcgacga ttccaggaat
     5461 aatcaccccc gcgccggctt ccctgacaca gacaaaaacc tgcccgttgg ccggacgttg
     5521 caggggcgct acaatcaggg cagcaaagtt atgaccattg cgggtttgct gcctgacaag
     5581 cgctttctgg gagcagactg ccgccactat tgccgccagg taccccataa gaggttccac
     5641 aatgactcgt caacagatga aactcatcgt gattggggcg agcacactgc tgaccgccgg
     5701 caccgcactg gcccacgttc cctacctgga gacgaccgac ttcacttggg agaagccctt
     5761 ccgggtgcgc ggatcgatcg cccaatcgat cgcggtttat gcatggcttg agatggccga
     5821 cggggcgacg gaagacatcg acgtctacca gttcaacctg cgccgctcga cccaggtctt
     5881 cgtcgagacc cttgtgccga tctgcccggc ctacgcggag ttccggccgt cttttgcgat
     5941 cgtcggcccc gggctcgatg tcccggccga gtcgctgcct tttgagcttc ccgtcggcta
     6001 cggcgcgatc gtcgtaccca actactctga tccgcaacga ccgaccttct acgagccgtt
     6061 cggcaacaag tcatactatg acggcccgga gttcaaacag gtgctctcga caccggggac
     6121 ctactacgtc gtggtctggg acccggccgg catcggcggc gactacgtgg ccgtgctggg
     6181 ggacaaggaa atctggtggc cctgggacat catccgggcc ctgatccaga cgcccaagat
     6241 ccgccggggg ctggaactgc acaccgactg caggtgacac gcagaacggg tgggaaaagg
     6301 tagggcatgg tcgaagccac caaaggtggc ggagacctgc cgggacgccc caccgcgaca
     6361 cggtgggggc cgaattggcg aagttgtgac cgaataatgc ggcaggtccc agtcgccgcg
     6421 gcgaccccgc ccgcggcgag gcaggctcga cgctgcccca tcgcccgccc ccgtcgcttt
     6481 tcacacacac cttcagatga aaccggtctt gcgtgcgatc ctgtgtaaaa tgtaacatct
     6541 gctgttggaa tttctaacag ccggtgttgg attctgcagg gccatcaatg aaaccgcatt
     6601 caggcaaacg cgctccgatc aagtggtacg gtgtgctgct gcagatatgc ttcgcccctg
     6661 tggctgtgct gatcaccttc gcggcgattc tcacggcgcc tgtcctcgtc ttcaagatga
     6721 tcacagagga gcgaaaacaa cgggaaaagg tgcggcagtt tctccgaggg cagggcgcgc
     6781 gggccttgct ggtctggcat tcgcggaggg ggtggaacga gttcggcgaa aagaaccttc
     6841 tgcctgcgct gccggaggga atcgtcgcgg tccacgatcg gcggcggcgt gactcgactt
     6901 acagggatcg gcgttcggcg gaacaggccg acggtgtgtc cgagttggct cgcccgtacc
     6961 tgcttttctt ccacaaggcc aggttgcagg cgatcagctt gaaccgggct ctgtggcccc
     7021 tcaagcgtca gcggaagccg agtctggccg tgcagcgcga ggtttcgcgg atcattctcg
     7081 accacgtcgc cgattcgccg atccgactcg attcagcacc tcgtgatggc tcaactgaca
     7141 tgcttccaat gcgtaattga gaatccggtc gccttgccgt acattagcgc cgcgatgacg
     7201 cgggaaggca ccaagccgaa atgtgagccg ccggccgagg ggctgacccc ggccatgcgc
     7261 cagtacattg agcagaaagc ggccgctccg gacgccctcc tgctctttcg catgggcgac
     7321 ttctacgaga cgttctacga tgatgcgatc accgccgcgc gcgtcttggg catcaccctg
     7381 accacacggg gcaaagaccg caacggtcag gccgtccctc tggccggcat tccctatcac
     7441 gctctcgaag gctatctcac gaaactggtg aaggccggtt tcaaggtcgc gatcagcgag
     7501 cagctcgaag atcccaagct ggccaagggc gtggtcaaac gggacatcgt ccgcatcgtg
     7561 acccccggga cgctcaccga tgacgccctg ctggaacgta aggcggacaa ctatctggtt
     7621 tgccttcacc atcgtcgcga cgagaccggg ttggcctgcg tcgagctgtc taccggcgca
     7681 ttctgggtgc agaccgtcgc tgcgaaggaa atactcaacg aactggtccg cctttcgccg
     7741 gccgaactac tcctggccga agtggccatc gatgtccgcg atccgttggc ccacgtggtg
     7801 aaggagctga cgggtccgaa cccgatcatc ccctcgctgc tggtgacccg tcggccggca
     7861 cacgtattcg atccctacca ggccgaccag cgtcttcggc ggcacttcgg cgtggctacg
     7921 cttgagggct tcggctttga tcgcatcgac gcctcgctct cggccgcggc agcgatcatc
     7981 gactatttgt cggagaccca gaaaggctcg ctcaaccata ttgttaaact gacccgccgc
     8041 gagacgaacg acttcgtgct gatcgaccag gccacctggc ggtcgctgga gatcgagcgg
     8101 acgatccgag gcggctcgac cagcggctcg ctgcttgcgg cagtggatcg tacttcgacg
     8161 ccgatgggcg ctcgctgtct gcgccgctgg ctggcagccc cgcttcgcga tgctggtcgg
     8221 atccgcgagc gccagcgggc catcgcggac ctgctgaccg accggcacct gctgcaaacg
     8281 cttcgcaccg agatgggcag gctggcggac atcgaacgga tcactgcaag gctgggcgtg
     8341 ggccgggctt cgccgcgcga ccttctggca ctcgggcaaa cgctgctcgg cgtggagagg
     8401 attgcggcga ctctcgacag gctggccgct cagaccagca tcaccggcga cagactctgt
     8461 gccttcctgg ccgaacgccg cgatgccctg cgcggcgagc acgacctggc cgagttcctc
     8521 accacggcct tgcgcccgaa tcccccgcag gtgctcaacg agggcgggat catcgccgac
     8581 ggctacgacg ccgaactgga ccggttgcga accatgagct cgaatgccca gcagtggctg
     8641 gccgactacc aggcccgcga aattcaacga accggtatca gctcactgaa ggtcggatac
     8701 aaccaggtgt tcggctacta catcgagatc acccacgcac acgcctcgcg gattccaccc
     8761 gattacgtcc gcaagcagac actcaagaac gccgagcggt acatcaccga cgaactcaag
     8821 caatacgaga ccgagatcct cacggcccgc gaccgggcca tcgcccgtga gcaggaattg
     8881 ttcgaggtca tccgccggcg ggccgttgag cggatcgctc cgctgcagca gcttgcggcg
     8941 gcgatatctg agatcgatgt ggtcgccggc cttgctcatc tggccgatga gcggcgatat
     9001 gtttgcccgg agatggtgga aggcaacgcc ctggagatct gcgaaggccg ccaccccgtg
     9061 ctggagcaga cgctggcgga gaagttcgtg ccgaatgact gccgactggg agcggtcagc
     9121 gatcagcagt cagcggtcag cggccggcga tcagcagtca gccagacaga ttgcgcaacc
     9181 agcggtcagc aatcagcagt cagccagaca gattcggccg cgatccgacc ggattctgtc
     9241 ggtctgttcg ggccgcaagc ggatctcaaa tctgaaatct cagatcggca atctgacatc
     9301 tcagatctca aatctcaaat ctcaaattct aaatccgaaa ctctggccat cctgaccggg
     9361 ccgaacatgg ccggcaagag cacctacatc cgccagattg ccctgctgac gatacttgct
     9421 cagacgggca gctacgtccc ggccgcgtcg atgcgattca cgccggccga ccggatcttc
     9481 gcccgcatcg gggcctccga cgaactgacc cgcggtcagt ccaccttcat ggtcgagatg
     9541 accgaggcgg ccaacatcct caacaacgcc tccgaagcca gcttggtgat catcgacgaa
     9601 ctcggccgcg gaacaagcac cttcgacggg ctggccttgg cctgggcgat cgccgagcaa
     9661 ctcgtcgtcg gcgtcggctg ccggacactc ttcgccacgc actaccatga gctgaccgag
     9721 ctggagaagt acatgaacgg cgcggtgaac tacaacgtcg cggtccgcga gtggcaggac
     9781 gaggtcgtct tcctccaccg catcgttcgc ggcaagactg accgcagcta cggcgtacac
     9841 gtcgccaagc tggccggcgt cggcccggag gtcatcgccc gaagccgcga gatcctggcc
     9901 gaattggagg ccaacttctc ggccacccgt cgggctccgc gccgcaccgc tcgccgtaac
     9961 cgacgcgacg atcaactctt gctctttgcc gacccggccg acgaggtcat caaggagatc
    10021 cgccaactcg atccccacaa gctgacgccg atcgacgctt tgcaggccat cgagaaatgg
    10081 cgcaagcgcc tggaggagtg aaaactggag tttggcattt ttgcggcgtg ctgatgctcg
    10141 cccttgaggc ctcggtccc
//
LOCUS       DAOTPW010000214        10025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6268_length_10025_cov_5.385356, whole genome shotgun sequence.
ACCESSION   DAOTPW010000214 DAOTPW010000000
VERSION     DAOTPW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6268_length_10025_cov_5.385356"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2477
                     /locus_tag="P5316_16825"
     CDS             <1..2477
                     /locus_tag="P5316_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86675.1"
                     /translation="LFQDASRIRRAMRTSEGAVTQPAEMEVISAPTPQQAAALKPRPT
                     VYFDGDNLEVDQRAGIVTVIGNVYVSQGLVDSKEVMELQTDAAVLFLARPPSGQPSDT
                     AAPGLQPVPAGRSGRTPEQLSASLGFGEGIGAQVSGVYLQGNVILSRGDRRIRATELY
                     YDFEHDRALILDAVMWTTIPDRDIPLYVRARQVRQLSTTDFVAKNAIITTSEFHTPHL
                     HLGAGEVKLTDAAFRDQSGQIAGIQAGKFQMRDATLNLEGIPIAYWPYVAGDFRRSET
                     SIHNVRTAYSDDFGATFESKWYLFNLLGIEKPDGVDGILRLDYYSKRGPGVGIDLDYE
                     LENAYGLIRSYYIYDRGNDNLGPFRDGTVEDPNRDRALIRHRQILPDGWELTLEASHI
                     SDRNFLEEYFRPEFEEGKEQETLLYLKKQQNNWAFTALAQWRILDFLTQTEHLPDLGF
                     HWVGQPLANIINYYNESHVGFVRYRGDEGRLFEYNREFDNTESTRLTFRGETRNEVDM
                     PIKLTSINLNIVPFATARAGYWDGSPRSGSVDRLFGMAGVRAGTQFWKLFENAVSELF
                     DINGVRHVIRPEVTAWGSASNRDSYDLTPFDHGIEDVDDFYGTSIALRQRWQTKRGGP
                     RTPGGEGEWRKVDWIVLDIELNLFGNSPLYELPIGRYYAVRPENSVARNHVRTDFLYR
                     ISDTTTILSDSNFDLTDGDLDLFNLSYAVERSPRFSYFVGYRRIHDTDSDLIGAGANY
                     GISTKYRVAVRTYYDIQRNRTDMFDISLIRKWPRWYSALTFQLDRLEDSFGVGLSVWP
                     EGAPQMALGSRRYTSLSESTGIKPED"
     gene            complement(2663..2893)
                     /locus_tag="P5316_16830"
     CDS             complement(2663..2893)
                     /locus_tag="P5316_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HRR86676.1"
                     /translation="MSLDPSLKVAGGLSRHRNVLTRDERLAKLREAERWEKGKSVFGL
                     PKVANRSIKVGKKAKKKEEAAPGTTEASAKSS"
     gene            complement(3080..4159)
                     /locus_tag="P5316_16835"
     CDS             complement(3080..4159)
                     /locus_tag="P5316_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86677.1"
                     /translation="MAKSKRAPALFEVIEPRHLQGGSSRFPLPKWWRGSKTSSPSYAP
                     AGRSEEKTADREKIIKLVPQFDRPSSQEKSSATASRSPVASSTQLQPSPPVTTAAARS
                     EERSTGSGSERGSGVTGGRAPVVGVDRGRIQFSLNPLSLCVAFGVLLIAVIGAYQFGR
                     ENRGAQPRVDDMPAALTGGEGGAMVPDARGPSSPARARAELTKGDVSPPVLLPAASGS
                     GKNVSAGQSNAGSASGRPPVTAPDAGSTSAGAAAIPTSVRVKGLNYLYIVRFRPEHLE
                     DAVHAHKWLASKGIQTALDKSDDWLSLVSADGFDLSSADDKAKRLALEAKLKDLTPAY
                     RQDCRAEQRFRYYAFDKPELRKQKD"
     gene            4424..5410
                     /locus_tag="P5316_16840"
     CDS             4424..5410
                     /locus_tag="P5316_16840"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRR86678.1"
                     /translation="MTAFAILPLSGGCTLPGRPAGPGRLLENRDPLFQRSYLLYLPSH
                     YDPQERWPLVIVCHSDGPFENARRQINEWKRLAEKEGFLLAAPDLKRSKGLGAEKPSA
                     EQITAEDEGFLLSALRSIRGAHTIDETRVFLAGTGAGAFPALYVGLRHPDIWRAICVR
                     QPSFDANRLDPCAPFLDPYQPIQVLYCTGDIIGRKKAEACVTWLQDHDLNLTAIEEPG
                     AHRRDPGAVFRFVTEVVRQQPWIRVHVRDDARNDMAISLAARMSFEPKRIRWDFGDGS
                     SSAELTPSHVYSQPGRYTVKLSVWSPGGAQHVRQLEVRIPRIRLGVTQPATS"
     gene            5423..6274
                     /locus_tag="P5316_16845"
     CDS             5423..6274
                     /locus_tag="P5316_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86679.1"
                     /translation="MAILGRRLERAPFWIRWPVKWIVFAAVVFAVCYPHPSLLRRHLR
                     NLKQIDKLPDPTEPMLQPMLRDLDEHLAACGVTTSAPAQWLRQVQVFVNHRIPYAFDW
                     DSWGVVDYLPTLPEIIQQGKEDCDGRAVVAAALIRAKIGEAQLVASSTHMWVSTPLGE
                     TMGPMGKPALASSAEGVRIRWTQFVNLTDFATGVALFPLAREAIIVLTAWLLLLPAQI
                     RNLQAFLCLLLLVEAWILFRLSGADPYQPSHWGARWALLHTLAAVVVISLNPRHFQKD
                     TDRTVSP"
     gene            6605..8506
                     /gene="sppA"
                     /locus_tag="P5316_16850"
     CDS             6605..8506
                     /gene="sppA"
                     /locus_tag="P5316_16850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRR86680.1"
                     /translation="MQRRKRVVGLLVGLALLAIVAAPAVADKKVDSTQADATKTATTK
                     PAKPAIIAVYNLRGELKDGPPTLAVSLDLRTPQSLFKLLERFRKIEKDNEVKAVLLSI
                     SDLALGWGQMQELRQAILGLRAAKKDVYCYLEDASPSVYLLATAASRISIVPTGEIAL
                     MGMHVEQAYFKGLMDKIGVEADIEHMGEFKGAGEPFTRTGPSEQARQMIEWLVKDLFE
                     QMIKTVSQGRDIPFDKVRSLIDQGPFNARQALEAKLVDHAIYADELVDALRDRYGDDA
                     RFVQNYGADKRAQLDLSSPFAIFKLLGESASKGKPSAKPAVAVVYLDGMIVTGRTEQS
                     PFGDAGTVGSTSLRHALARAASDKSVKAVVLRVNSPGGSATASDIIWRAASELGKEKP
                     LVVSMGDMAASGGYYVSAGARAIFADRGTITGSIGVLTGKIVTKGLWDWVGITFHETT
                     MGQNADLFNTNRRFDDRQRAIVREHLENIYKEFTDRVMAGRGNKLKKDLSELAGGRVF
                     TGRQAEANGLVDRLGGLQDAVSYAAKLADLSEDEYELRVLPEPKNFMDLFIRSLTGQE
                     EDDEGVDVAVTIDEPWTLKLPFARELLPALKALDPDRAKLVLRSLLRIELLGREHVLL
                     ALPCEISIR"
     gene            8518..9654
                     /locus_tag="P5316_16855"
     CDS             8518..9654
                     /locus_tag="P5316_16855"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR86681.1"
                     /translation="MGGTRSIDWREQIELIIDGRQPRYLPAVFRMSRWYKARIHRNSL
                     PAELAGMSLAQIEDYLGLARSARYAKICRIEFRPPVEKLLYRDGDLVVTEYRTPRGDL
                     RRIARFGPGDETSGIDPTLIEYPVKSWKDYAALAEVFRHMEFVPTYDEYNRYDRQIGR
                     AGMPMVIIGAIPFHYVLQEWTGYEKGYLDLHDRPDVFLEAVEAGNQAYVRMWEVAASS
                     PARLLLHGINFDRQMTPPPLWRQHWLDYLSRFTREMHLHGKKVVFHADGDMSGLLELM
                     LEADLDVADCFACHPLVPCTVAQAREVWKDRITIWGGLPSTLLEPGAPIDALLAHLDD
                     LRKTVHPGKRFILGLTDMAMPTTSWDHLLAVRDWLAQHRDWLVG"
     gene            9802..>10025
                     /locus_tag="P5316_16860"
     CDS             9802..>10025
                     /locus_tag="P5316_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86682.1"
                     /translation="MAGRITNGLNLARASWEVLKLDKEMLLFPVLSGLACLVVIASFA
                     APLVAGNHLQTITVTVQTPSVRSAGSIPPF"
BASE COUNT         2008 a         3230 c         2852 g         1935 t
ORIGIN      
        1 agttgtttca agatgcgtcg agaatccgtc gtgccatgcg tacttccgag ggtgctgtga
       61 ctcaacctgc cgagatggaa gtcatctccg ccccgactcc ccagcaggca gctgccctca
      121 aaccccggcc gacggtgtac ttcgacggcg ataacctcga ggttgaccag cgagccggca
      181 ttgtcaccgt catcggcaat gtctacgtct cgcaaggtct cgtcgacagc aaggaagtga
      241 tggagcttca gaccgatgcg gcggtcctct tcctggctcg cccgccatcg ggccagcctt
      301 ccgacaccgc cgctcccggt ctgcagcccg tgcccgcggg gcgaagcgga cggactcccg
      361 aacaactgtc ggcgtcgctc ggctttggcg agggcattgg cgcgcaggtc tcgggtgtct
      421 atctgcaggg aaacgtcatt ctctcccgcg gtgaccgccg tatccgagcc acggaactct
      481 attacgactt cgagcacgac cgggcgctga ttcttgacgc cgtcatgtgg acaaccatcc
      541 ccgaccgcga cattcccctt tacgtgcggg cgcggcaagt ccggcagctt tcgactacgg
      601 acttcgtcgc caagaacgcg atcatcacca ccagtgagtt ccatacgccg cacctgcatc
      661 tgggtgccgg cgaggtgaaa ctgactgacg ccgcttttcg cgaccaatcc ggccaaatcg
      721 cgggcatcca ggccggcaag ttccagatgc gggatgccac cctgaacctc gaaggcattc
      781 cgatcgctta ttggccttat gtcgccggcg atttccggcg gagcgagact tccatccata
      841 acgttcggac cgcctacagc gacgacttcg gggcgacctt cgagtcaaag tggtacctgt
      901 tcaatcttct cggcatcgag aagcccgacg gcgtggacgg catcctgcgc ctcgactact
      961 acagcaaacg cggccctggc gtcggcatcg acctcgacta cgagctggag aacgcctacg
     1021 gcctgatccg cagctactac atctacgacc gcggtaatga taatctcgga ccattccgcg
     1081 acggcaccgt cgaagacccc aaccgtgaca gggccctgat ccgacaccgt cagattctgc
     1141 ccgacggctg ggagctgacc ctcgaagctt cgcacatcag cgaccgcaat ttcctcgagg
     1201 agtacttccg acccgagttt gaggaaggca aagagcagga aacgctgctc tatctcaaga
     1261 agcagcaaaa caactgggca ttcaccgccc tcgcgcaatg gcggatactc gacttcctca
     1321 cccagaccga gcacctgccc gacttgggct tccactgggt gggtcagccg ctggccaata
     1381 tcatcaacta ttacaacgaa tcacatgtcg gcttcgtccg gtatcgtggc gatgaaggcc
     1441 ggttgttcga gtacaaccgc gagtttgaca acaccgagtc cacccgtctc accttccgag
     1501 gcgaaacgcg caacgaggtc gacatgccga tcaagctcac gagcatcaac ctcaacatcg
     1561 tcccgttcgc cacggctcgg gcggggtatt gggacggcag cccgcgaagc ggctcggtcg
     1621 accgcctctt cggcatggcc ggcgtgaggg cgggaactca gttttggaag ctcttcgaga
     1681 acgccgtttc ggaactcttc gacatcaacg gcgttcggca cgttatcagg cctgaggtaa
     1741 ccgcttgggg cagcgccagc aatcgcgaca gctacgatct gacgcctttc gaccacggca
     1801 tcgaagacgt cgacgatttc tacggcacgt caatcgccct gcgacagcga tggcaaacca
     1861 aacgcggcgg accgcgaact ccgggcggtg agggcgaatg gcgaaaggtg gattggatcg
     1921 tcctggatat cgagctcaac ctattcggga acagcccgct ctacgaattg ccgatcggcc
     1981 ggtactacgc cgtgcgaccg gaaaacagtg tcgcccgcaa tcacgtgcgc accgacttcc
     2041 tgtaccgcat cagcgacacg accaccatcc tgtcggattc caatttcgac ctgaccgacg
     2101 gcgacttgga cctgttcaat ctctcctatg ctgtcgagcg ctcgccgcgt ttctcttact
     2161 tcgtgggata ccggcgaatc cacgacaccg actccgacct gatcggtgcc ggagccaact
     2221 acgggatcag caccaagtac cgtgtagcgg tccgcaccta ctacgacatc cagcggaacc
     2281 gcaccgatat gttcgatatc agcctcatcc ggaagtggcc gaggtggtac agcgccctga
     2341 cgtttcaact cgacaggttg gaagacagtt tcggggtggg actgtccgta tggccagaag
     2401 gcgcaccaca aatggccctg ggatcccggc gatacaccag cctgtcggaa tccaccggga
     2461 tcaaacctga agactgaagc tctctcccag gcaagaggaa ggggaacccg cttgcgcccg
     2521 gcacccactt tccctccaag acatgcggct caaccgtgtt ccgcacaggc ctgcggcgca
     2581 acaatgaacc acggtcggca acgaccgctc atgcgacctg atgtaacggg aaataccgcc
     2641 gaacaaccgc ctgagcactg gatcaggacg acttggcgga ggcctcggtc gtccctgggg
     2701 cagcctcttc cttcttcttg gccttcttac cgaccttgat actccgattg gctaccttcg
     2761 gcaggccgaa aacgctcttg cctttctccc atcgctcggc ttctctgagc tttgcaaggc
     2821 gctcgtcgcg ggtcaggaca ttacgatggc ggcttagacc gccggcaacc ttcaatgatg
     2881 gatcaagcga catacacttc ggtctcccat tcgcccggtg tcagtacgaa caagctagga
     2941 taccatacgg cggcctttca gcaaagcggg caaatgcggc gggacattag aagcttacgg
     3001 ccagacatct gtgcggcttg cgcgcatgtt accggatcgc ggtgcgcttg ggttgcggcc
     3061 cgcaaaaagg cagtccgcct tagtctttct gtttccgcaa ttcaggcttg tcaaacgcgt
     3121 agtatcggaa ccgctgctcg gcccgacagt cctgccggta agccggagtc agatctttga
     3181 gcttggcttc cagagccaat cgcttggctt tgtcatccgc gctgctcagg tcgaaaccgt
     3241 ccgcgctcac cagggacaac cagtcgtcac tcttgtccaa ggccgtctgt atgcccttac
     3301 tggccaacca cttatgagca tgaacagcat cttccaggtg ctccggtctg aaccgtacga
     3361 tgtagagata gttgagaccc ttaacgcgga ccgacgtcgg aatcgctgcc gcccccgcac
     3421 tggttgaacc ggcgtcagga gccgtgacgg ggggtcttcc cgaggcgcta ccggcgtttg
     3481 actgaccggc agatacgttc tttcccgaac cgcttgctgc aggaagcagt accggcgggc
     3541 tcacgtcgcc ctttgtgagt tcggcgcgag ccctggctgg gctgctcggt ccacgggcat
     3601 caggcaccat cgcgcccccc tcccctcccg tcaaagctgc cggcatgtca tccaccctcg
     3661 gctgagcgcc tcgattctcc cgcccgaatt gataagcgcc gatcacggcg ataagcagaa
     3721 caccgaacgc gacgcacagg ctgagcggat tcagtgaaaa ctgaatcctt cccctatcca
     3781 cgccaaccac aggcgccctg ccgcccgtaa cgcccgagcc gcgctcgctt ccgctgcccg
     3841 tagagcgttc ctcagacctg gccgcggccg ttgtcaccgg cggcgaaggc tgaagctgcg
     3901 ttgatgacgc aacaggcgat ctcgaagcag tcgccgacga cttctcttgg ctgctcggcc
     3961 tgtcgaattg gggcaccaac ttgatgatct tctcccgatc ggccgttttc tcttccgacc
     4021 ggccggccgg ggcatagctc ggactgctgg ttttgcttcc gcgccaccac ttgggaagcg
     4081 ggaatctgct tgaaccgccc tgcaagtgcc gaggttcaat cacctcgaac aatgccggcg
     4141 ctctcttgga ttttgccacg tgagtgaccc tcgattaggc ttgccgcgcg tgcttccctc
     4201 gcactgtttc gctaaacgga tacgatagcc ttttttgagc cggattcaag agactacttg
     4261 agctttctca caattgttgc caatatcgaa gcaacagctc tcaaagcggc cggcgtgttg
     4321 ataaatcccg ctcaaagctg ataatccgaa aaccgaaaac acgctctcac caggaggaac
     4381 ggccgtgcac ggcaataaat gcgcgttgac gacagcctgc ctcatgacgg cattcgcaat
     4441 cttgccgctt tccgggggct gcacattgcc cggccgccca gccggtcccg ggcgactgct
     4501 tgaaaaccgc gacccattgt tccagcgatc gtatctgctc tatttgccga gccactacga
     4561 cccgcaggag cgatggccgt tggtgatcgt ctgccacagt gacggcccgt tcgagaatgc
     4621 ccgtcggcaa atcaacgaat ggaaacgact ggcggagaaa gagggattcc ttctggctgc
     4681 tcccgatctc aagcggagca agggcctcgg cgccgaaaag ccttcggcag agcagatcac
     4741 tgcggaggac gaaggattcc tcctttcggc gctccgaagc attcgtgggg cccacaccat
     4801 cgacgaaacg cgcgtcttcc tcgccggcac cggagcgggg gcattccctg ccctctatgt
     4861 cggccttcgg catcccgaca tctggcgtgc gatctgtgtg cgacaaccct ccttcgacgc
     4921 gaatcggctc gatccatgcg cgccattcct ggacccctac caacccatcc aagtcctgta
     4981 ttgcaccggc gacatcatcg gcaggaagaa ggccgaagcc tgtgtcactt ggctgcaaga
     5041 ccatgatttg aacctgacgg ccatcgaaga gccgggggcc catcgccgcg atcccggagc
     5101 cgtgttccgt ttcgtcaccg aggtcgtccg gcaacagccg tggattcgcg tccatgtccg
     5161 cgacgacgca agaaacgaca tggcgatcag cctggcggcc agaatgtcct ttgagcccaa
     5221 aaggatcaga tgggatttcg gcgacggcag cagctcggcc gagttgacac catcccacgt
     5281 ctacagccaa ccggggcggt ataccgtcaa attgtcggtc tggtcgcccg gcggtgcgca
     5341 acacgttcgt caactcgagg tgcgaatccc tcgaattcgc cttggagtca cgcaacctgc
     5401 cacatcttga ggaatcgttg acatggcaat actcggccgg cgactggaac gtgctccttt
     5461 ctggatccgc tggcccgtca agtggatcgt ctttgcagcc gtcgtgttcg cggtctgcta
     5521 cccccatcct tccctgctcc gccgccacct gcgcaacctc aagcagatcg acaagctccc
     5581 tgatcccacc gagcccatgc tgcaacccat gcttcgcgat ctggacgaac atctggcagc
     5641 ctgtggcgtg acaacttcgg ctcccgccca gtggctcaga caggtacagg tgtttgtgaa
     5701 ccaccgaatc ccttatgcat ttgactggga ttcgtggggc gtcgtggact atttgcccac
     5761 cctgccggaa atcatccagc agggcaagga agactgcgac ggccgggccg tggtcgccgc
     5821 cgcactcatc cgagccaaga tcggagaggc ccaactggtc gcctcctcca cccatatgtg
     5881 ggtctcaaca ccccttggcg aaaccatggg accaatgggc aagcccgcac tagcatcctc
     5941 tgccgaaggc gttcgcatcc gatggacaca gttcgtcaac ctcaccgatt tcgcaaccgg
     6001 tgtcgccctc tttcccctcg cccgcgaagc gatcatcgtc ctgacggcgt ggctcctatt
     6061 gctgcccgca caaatccgaa atctccaggc gtttctgtgc ctgctcctgc tcgtcgaggc
     6121 ctggattctc ttccggctgt cgggagccga cccctaccag cccagtcact ggggcgcccg
     6181 ttgggctctg ctccacacct tggccgccgt tgtggtcatc agcctgaatc cccggcattt
     6241 ccaaaaagat accgaccgga cggtttctcc ctagtttcct cctgattccg tgacccttgc
     6301 agccacgccg gcctttcacg gttgtattat gttgcgcgga aatgacttat agacaccgct
     6361 ctccggtttc cattcgctgc tgcggctcga cgtttgttcg tcagacgttg ttgcagtcac
     6421 agactggcca aatctggcaa aactgagata atgagtgatc agggtaatct tggtgtggtc
     6481 ggcaacgaca gtctaccctg ccacgaggat tgccggatcg cgcgtcaggc ggcgaaacaa
     6541 acgctggcct gcgccgacca cacggatagg tgtgaagaat cggctctttc aggagagaca
     6601 tgccatgcaa cgaaggaaac gggttgtcgg actgctggtc ggactggctt tgcttgccat
     6661 tgtggccgcc ccggcggtcg ctgacaagaa agtggacagc actcaagcgg acgctaccaa
     6721 gacggcaacc accaaaccgg ccaagccggc aatcattgcg gtctataacc tccgcggtga
     6781 actcaaggat ggtcccccca cattggccgt cagcctcgac ctgcggaccc cgcaatcgtt
     6841 gttcaagctg ctcgagcggt ttcgcaagat cgagaaggat aatgaggtaa aggccgttct
     6901 cctctccata tccgatcttg ccttgggatg gggccagatg caggagctgc gccaggcgat
     6961 cctcgggctg cgggcggcca agaaggatgt gtattgttat ctcgaagatg cctcgccctc
     7021 agtctacctg ctggccaccg cggcatcacg gatcagtatc gtgcccacgg gagaaatagc
     7081 cctgatgggc atgcatgtcg agcaggccta cttcaagggg ctcatggata agatcggcgt
     7141 tgaagccgac atcgagcaca tgggtgagtt caagggcgcc ggcgaaccgt tcacccgaac
     7201 cggaccgtcc gaacaggcca ggcagatgat cgaatggctg gtcaaggacc tcttcgaaca
     7261 aatgattaaa acggtttcac aaggacggga cattccgttt gacaaggttc gctcgctcat
     7321 cgaccaggga ccattcaacg cccggcaggc ccttgaagcc aaactggtcg accacgccat
     7381 ttatgccgat gagcttgtgg acgccttgcg cgatcgctac ggcgacgatg ccagattcgt
     7441 ccagaactat ggcgccgaca agagggcaca gctcgatctg tcgagtcctt tcgcgatctt
     7501 caagttgttg ggagaaagcg cgtccaaagg caaaccgtca gccaagcccg ctgtcgccgt
     7561 ggtctacctg gacggcatga tcgtcaccgg ccggacggaa caaagcccct tcggcgacgc
     7621 cgggaccgtg ggcagcacca gtctgcgaca cgcattggcc agggcggcct cagacaagtc
     7681 cgtcaaggcg gtcgttctcc gcgtcaactc gcccggcggt tccgcaaccg ccagcgatat
     7741 catctggcgc gcggccagtg aactcggaaa ggaaaagccc ttggtggtct cgatgggcga
     7801 catggcggcg agcggcggtt actacgtctc cgccggggcg agggccattt tcgctgaccg
     7861 cggcaccatc accggttcga tcggggtcct gaccggcaag atcgtcacca aggggctgtg
     7921 ggattgggtc ggcatcacct tccacgagac aaccatgggc cagaacgccg acctgttcaa
     7981 caccaatcgc cgcttcgacg atcgtcagcg agccatcgtg cgagagcatc tcgagaacat
     8041 ttacaaggag ttcaccgacc gcgtcatggc cggtcgtggc aacaaactga agaaggatct
     8101 ttccgaactg gcaggcggcc gggtcttcac cggccgtcag gcggaagcta acggcctggt
     8161 cgaccggctc ggcggcctcc aggacgccgt ctcctacgcc gccaaactgg ccgacctcag
     8221 cgaagatgaa tatgagctgc gcgtgctgcc cgaacccaag aacttcatgg atctgttcat
     8281 ccgcagcctt accggccagg aggaagacga cgaaggcgtg gatgtggccg tcacgatcga
     8341 tgaaccatgg actctgaaac tcccattcgc tcgtgaattg ctgccggcac tcaaggccct
     8401 cgaccctgat cgagcaaagc tcgttctccg atcgctcctg cgcatcgagc tgctcggccg
     8461 cgaacacgtt ctgctggccc tgccgtgcga gatctcgatc cggtaaactg agcgagaatg
     8521 ggcggcactc ggtccattga ctggcgcgag cagatcgagc tgatcatcga cggccggcaa
     8581 ccgcgttatt tgccggccgt cttccggatg agccgctggt acaaggcccg gatccaccgc
     8641 aacagcctgc cggccgagct tgcgggcatg tcgctggcac agatcgagga ctacctgggc
     8701 ctggcccggt ccgctcgcta cgcgaagatc tgccgtatcg agttccgccc gcccgtcgaa
     8761 aagctgctgt accgcgacgg cgacctggtt gtcaccgaat accgcacccc tcgcggcgat
     8821 ttgcggcgga tcgccagatt cgggccgggc gacgagacct cgggcatcga tccgaccctc
     8881 atcgagtatc cggtcaagag ctggaaagat tacgccgctc tggccgaggt tttccgacac
     8941 atggagttcg tccccacgta cgacgagtac aaccgatacg accgccagat cggccgggca
     9001 ggcatgccca tggtcatcat tggggcaatt ccgtttcact acgtccttca ggagtggacg
     9061 ggctacgaga agggatacct tgacttgcat gaccggccgg acgttttcct ggaagccgtt
     9121 gaggcaggca atcaagccta cgtcagaatg tgggaggtgg ccgcatcctc gccggccaga
     9181 ctcttgctgc acggcatcaa tttcgatcgg cagatgacgc cgccgcccct ctggcggcag
     9241 cactggctgg actatctgag ccgcttcacc agggaaatgc accttcacgg caagaaggtc
     9301 gtttttcatg ccgacggcga catgagcggc ttgctcgaac tgatgctcga agccgatctc
     9361 gacgtcgccg actgctttgc ctgccatccc ctggtgccgt gcacggtcgc ccaggcacgc
     9421 gaggtgtgga aagaccgcat caccatttgg ggcggcctgc cttccacgct cctggaaccc
     9481 ggcgcgccca tcgacgccct gttggcccac ctggatgacc tccgcaagac agtccatcct
     9541 ggaaaacgct ttatcctcgg cctgaccgat atggccatgc cgacgacgag ttgggaccac
     9601 ctcctggccg tgcgtgactg gctggcacag cacagggact ggctggtagg atagtgtggg
     9661 accatggcgg gctgttgcgg cttgctgaag acagagggaa cggccgcccc gccgccagtt
     9721 gtgcaacaca tggagtgggg gtccgttttc gcctggcggc tgcgatgacc ctgaacaacg
     9781 ccgtggcaag gaggcgcaca tatggccggc agaatcacca acggtcttaa cctcgctcgg
     9841 gcaagttggg aagtcctcaa actcgataaa gagatgctct tgttcccggt gctcagcggc
     9901 ctcgcatgct tggtcgtaat cgccagtttt gccgcgccgc tcgtggcagg caaccacctg
     9961 caaacaatta ccgtaaccgt tcagacccca tcggtccgat ctgccggtag catacccccc
    10021 ttcaa
//
LOCUS       DAOTPW010000215         9965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6298_length_9965_cov_5.505853, whole genome shotgun sequence.
ACCESSION   DAOTPW010000215 DAOTPW010000000
VERSION     DAOTPW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_9965_cov_5.505853"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1536)
                     /locus_tag="P5316_16865"
     CDS             complement(187..1536)
                     /locus_tag="P5316_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86683.1"
                     /translation="MNRRELLKAGVVGSITSAFGGYFVQGAEQKPLRVGLIGTGWYGK
                     SDLFRLIQVAPVEVVSLCDVDKRLLAEAADLVAARQASKKRPRTYGDYREMLKEKDLD
                     IVLIGTPDHWHALTMIAAVEAGAHVYVQKPISVDVAEGAAMVAAARKQGRVVQVGTQR
                     KSTPHLIDCKKNVVEAGLLGKVGHVEVCCYFHMRANGNPPVQPVPDYLDYEMWTGPAP
                     LRPYDGIPHRRWWRTFMEYGNGIVGDMCIHMLDTVRWMLGLGWPKRVSSAGGILVQKD
                     GKSNISDTQTATFEYDGLNVVWTHRTWGHAPDPKYPWALIIYGDDGTLKASTMSYDFY
                     PAGKNEPSIHKDVVYEREQYPEDLTEKDIELNAAPATRLHMKDFLNAIATHGKPVADI
                     EEGHISTASCILANIAMRVGRTLAWDAAKGQVVGDDEANRLLKRAYRTPWVHPADKT"
     gene            complement(1549..3150)
                     /gene="serA"
                     /locus_tag="P5316_16870"
     CDS             complement(1549..3150)
                     /gene="serA"
                     /locus_tag="P5316_16870"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRR86684.1"
                     /translation="MIKILVADPLAKEGMKKIQETSGIEADVKTGLKEDELAAIVGEY
                     DGMIIRSGVKITAKVLANPGKLRVIARAGVGVDNVDLQAATRAGILVMNTPDANTVST
                     AEQTMALMLALSRHVPKADAHVREKKWDRKALTGTQLAGKTLGIIGLGRVGSAVARRA
                     LAFDMKVIAYDPFFSGETALDGQVILCRTREEVFAKADYLTLHTKLSDETRQMINKEN
                     IAKMKKGVRIINSSRGGVINDADLAEALKSGHVAGAAIDVYTTEPPAPDNPLLTAPNT
                     VLSPHLGASTEEAQLQVTLDAVEILLNYLLKGEIRWACNVAGLPPQLTARDKAYLDLA
                     QRMGAILSHVGSGVIETVSVTTHGESLEPLLGTIQKQILIDVLSPHFTSRLNMINVDS
                     FVKERGIKLEQTADLTASAVTDSVTVEVKTRDGAHEVTGEVFLDGKPRIMAINGYQMN
                     LKPEGPMVLIFNDDQPGVIGLVGTVFGKNGINIADMMLSRRDNTALMVLKLDEAIPSK
                     VLQDLESHKPPIQRVLPVTLPPITV"
     gene            3319..4170
                     /locus_tag="P5316_16875"
     CDS             3319..4170
                     /locus_tag="P5316_16875"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRR86685.1"
                     /translation="MNLNAEPVKVAGGFKFCEGPAFDPKGRLWVVNLHGGFISRVTMS
                     GKKTRVAETPGPNGGQFDADGHYICCECKRKAIVRVSPEGIISALTESYEGHPFNGPN
                     DVAIDADGGFYFTDPDGSSLENRIGAVYYVRPDRTILQVDSGLAYPNGINITVDRSAV
                     LVAETLTHRVYRYRRRPDGTLGSRHVFCQLSGGVGPDGMCFDQDGNLYVAWYGSACVY
                     VVDPSGRPIGRLVTPGDNPTNCCFGPPGSKYAASLFVTETVTNAVWRYDIGVPGMPLH
                     HLAVASE"
     gene            4370..5191
                     /gene="proC"
                     /locus_tag="P5316_16880"
     CDS             4370..5191
                     /gene="proC"
                     /locus_tag="P5316_16880"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRR86686.1"
                     /translation="MPRRYEYGFIGAGNMAEGIISAAVGNRLFKSSRLAVYDPAEQRR
                     ELMVQKFGVAAVRDSFQLVAEAQRIVLAVKPQSFAEAITPLADHVRQDQLIVSIMAGV
                     STGRVESLFPQINLRVVRVMPNLPILVGAGVAGVCPGRFATAQDVADVQALFDAGGAS
                     VVVKDESLIDAVTAISGSGPAYFYYFVEAMVAGGVACGLSEAEALKLAAHTCLGAARM
                     MLETGEPPAELRRKVTSKGGTTQAALDAMNAAGVDKAIREAVLAAFDRAKELGKN"
     gene            5360..5698
                     /locus_tag="P5316_16885"
     CDS             5360..5698
                     /locus_tag="P5316_16885"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HRR86687.1"
                     /translation="MQRFGAVIGVKKEAIPEYKRLHADTWPSVLQVIRECNIRNYSIF
                     LKEVEKDKWYLFAYYEYTGEDIKADMEKMKTYKAMQDWWKLTDPLQEPLPIREKGEWW
                     AVGEEVFHTD"
     gene            5872..7686
                     /locus_tag="P5316_16890"
     CDS             5872..7686
                     /locus_tag="P5316_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240520.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRR86688.1"
                     /translation="MLALQLAIKGLDLAIIIVYFVVIIGIGCLAGLYRRRASEGKNYF
                     LAGGTLTWPIIGLALFSTNISTIHLVSQAQVGYLSGLAYGSFEWMAPFTLICLAMFFA
                     PFYLRSGVTTLPDFLEKRYSRASRNWLALLSMISAIFIHIGFSLYTGALVLNKMFGID
                     TMQSVIAICLLTGLYTIIGGLLAVVLTESLQTIILLCGAIAITLIGLTKAGGWSGMAS
                     SVDPVYLNVMRTAEQLPELPWYAVLLGYPVIGLWYWCADQTIVQRVLGAKNENHARVG
                     PLFAGFIKILPVFIFVMPGTIGLALIHQNKMPALPVAAAQEESGAASRPLTTAEQRVY
                     DHLTKISAGPDKAVSREDLSKAVDLTKAEIDGAVAGLTELKLVRDKSEDIYAHMIQHL
                     LPTGLKGIVAAALLAALMSTVSGALNSIATLFCYDIYRQWRPQVSDHKLVTLGRVVTF
                     VAMVLSILWTPYIARFENIYKGANSLICYIAPPITVVFLWGVFWRRASAKGAFATLVA
                     GSVLGLTVFLLEWFKDYTGWSLNFMMAAFYLFVVCSVVLVVVSLLRPQPADHPGNALV
                     WKHPLEALQGEAWRGIGNYKFLAGLLFITMVALYIIFG"
     gene            7729..8739
                     /locus_tag="P5316_16895"
     CDS             7729..8739
                     /locus_tag="P5316_16895"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRR86689.1"
                     /translation="MRFAAALSVLAAGLLVLGCGKSDSPEAGGKTSGLRFAVIPKGLT
                     HEHWKAVEAGAKKAATEVGNVEIIYKSAPKEDDTQLQINLVESFVSSRVDGIILAPLS
                     DQALVRPVRLAVQAKIPVIIFDSPLAGQVGKDFVCYIGTDNYRAGTLAGQRLGEVMEG
                     KGTVLLLRYAESSASTREREQGFLDALKKDFADIAVIDPPQYAGADVNSGKKAAENMI
                     TAHLGKFEGVFCPNETSAAGMLIALEDRQLAGKARFVGFDANPRVIAGLRDRKLDGVV
                     LQDPFKMGYQSVKQMLDHLAGKPVPPSTDTGAAVATPENVDSPEIYRLLHPLEAAVSR
                     GS"
     gene            8754..9758
                     /locus_tag="P5316_16900"
     CDS             8754..9758
                     /locus_tag="P5316_16900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR86690.1"
                     /translation="MVASRSRTAFNVGVILDFWARHKWVTPVVGLLGVVVFFALKSDN
                     FAFLGSDNMRIVAAQTVQVGVCAIGMTFIMIGGGIDLSVGSVMALAAVTTALTLKAGY
                     SCPTAVLVAMMTGALCGLANALLITGLRIVPFIATLGTLSAIRGLTRRLADNQVVRPP
                     NEAESLVSFVQPLGSSTWLAPAIWMTLALGVAAAVVLNWTAFGRRTFALGSNELAARL
                     SGIRVNRQKVYLYTLGGLFAGLAGLLAFANMREGDPTASAGIELQVIAAVVVGGGSLM
                     GGQGSILGTLVGAFMLTSLVNGCTLAGWENYVQEIAIGAIIVMAVALDYFRRRRLTER
                     "
     gene            complement(9782..>9965)
                     /locus_tag="P5316_16905"
     CDS             complement(9782..>9965)
                     /locus_tag="P5316_16905"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HRR86691.1"
                     /translation="VRLYEYAGRGETADLQIPEGFAKAQAVNLLERQGSPLSVKDRSV
                     QIEMKPFKLVTVRAER"
BASE COUNT         1742 a         2917 c         3229 g         2077 t
ORIGIN      
        1 atctcaaatc cccccgcccc gtcccaacct tcgcccgccg ccgcgccgcc gccaggcata
       61 agactaataa ggcgtatata ggctctatat aactctttta ggtgctatgc cctggggccc
      121 gacggcgccc cccgcgcccc ccgaacgcgc ccacgggcct gaaccggacg ggtgtgcctg
      181 accgtcctat gtcttatccg ccggatgtac ccacggtgtc cggtaggccc gcttgagcag
      241 gcggttggct tcgtcatccc cgaccacctg tcctttggcg gcgtcccagg ccagtgtgcg
      301 cccgactctc atggcgatgt tggccaggat gcaggaagcg gtggaaatgt ggccctcctc
      361 gatgtcggcc acgggtttgc cgtgtgtggc gatggcgttc aagaagtcct tcatgtgcaa
      421 ccgagtggcc ggcgcggcgt tcaactcgat gtccttctcg gtcagatcct ccgggtattg
      481 ctcgcgctcg tagaccacgt ccttgtggat gctgggctcg ttcttgcccg caggataaaa
      541 gtcatagctc atggtgctgg ccttgagggt gccgtcgtcg ccgtaaatga tcaaggccca
      601 cgggtacttg ggatcaggag cgtgccccca ggtacggtgc gtccagacga cgttcagacc
      661 gtcgtactca aacgtggcgg tctgagtgtc gctgatgttg gacttgccgt ccttttggac
      721 gaggatgccg ccggcggacg agacgcgctt cggccaaccc aggccgagca tccagcggac
      781 ggtgtccaac atgtggatgc acatgtcccc gacgatgccg ttgccgtatt ccatgaaggt
      841 tcgccaccaa cggcggtggg gaatgccgtc gtacggccgc aggggcgcgg gtccggtcca
      901 catctcgtaa tcgaggtagt ccggcacggg ctgcaccggc gggttgccgt tcgctcgcat
      961 gtggaaatag caacacacct ccacgtgacc aaccttgccc agaaggccgg cctctacgac
     1021 gttcttcttg cagtcgatca ggtgcggcgt gctcttacgc tgggtgccga cctggacgac
     1081 gcggccctgc ttacgcgcgg ctgcgaccat tgccgcccct tcggccacgt cgacgctgat
     1141 cggcttctgg acgtagacat gagccccggc ttcgaccgcc gcgatcatgg tcagggcgtg
     1201 ccagtgatcg ggcgtgccga tcagaacgat gtccagatcc ttttctttga gcatttcgcg
     1261 atagtcgccg taggtgcgag gccgtttctt cgaggcctga cgggcggcga cgaggtccgc
     1321 cgcttccgcg agcagcctct tgtcgacgtc gcacaacgag acgacctcga ccggggcaac
     1381 ctggatgagc cggaaaagat cggacttgcc gtaccagccg gtgccaatga gccccacgcg
     1441 taagggtttt tgttccgcgc cctggacgaa gtaaccgccg aaggcggatg tgatcgatcc
     1501 gactacgccg gccttgagca actcgcgacg attcatgtca agtcctctct ataccgtaat
     1561 gggcggcagt gttaccggca gaacacgctg gatcggcggc ttgtggctct ccaggtcctg
     1621 cagcaccttg gacggaatcg cctcgtcgag cttgagaacc atgagagccg tgttgtcccg
     1681 ccgagagagc atcatgtcgg cgatgttgat cccgttcttg ccgaagacgg tgcctaccag
     1741 gccgatcacg ccgggttggt cgtcgttgaa gatcagaacc atggggcctt cgggtttcag
     1801 gttcatctga tagccgttta tcgccatgat cctgggtttg ccgtcaagga agacctcccc
     1861 ggtcacctcg tgggcgccgt cccgcgtctt cacctccacg gtgacgctgt ccgtcacggc
     1921 gctggctgtc aggtcggccg tctgctcaag cttgataccg cgctctttca cgaagctgtc
     1981 tacgttgatc atgttcagcc gcgaggtgaa atgggggctg agcacgtcaa tgagtatctg
     2041 cttctgaatg gtgcccagca gaggctccaa agactcgccg tgggtggtga cgctcaccgt
     2101 ctcgatcact cccgaaccga cgtgcgatag gatcgccccc attcgctggg cgaggtccag
     2161 ataggccttg tcgcgagcgg tgagctgcgg cggcaagccg gccacgttac aggcccagcg
     2221 gatttcacct ttgagcagat agttgagcag aatctcaacc gcgtcgaggg ttacctgcaa
     2281 ctgtgcctct tccgtcgaag ccccgaggtg gggggacagt accgtgtttg gagccgtcag
     2341 cagcgggttg tcgggtgccg gcggctccgt cgtatagacg tcgatggccg ccccggccac
     2401 atgaccgctc ttgagggcct cggccagatc tgcgtcattg atcacgccgc cgcgggagct
     2461 gttgatgatc cgcaccccct tcttcatttt ggcgatgttc tctttgttga tcatctgcct
     2521 cgtttcgtca ctgagcttgg tatgaagggt gaggtaatcg gccttggcga agacctcctc
     2581 ccgcgtgcgg caaagaatga cttgtccgtc caacgctgtt tccccggaga aaaacgggtc
     2641 gtacgcaatg accttcatat cgaaggccaa agcccggcga gccaccgcgc tgcccacgcg
     2701 ccccaagccg atgattccca gcgtcttgcc ggccaactgt gttccggtga gagccttgcg
     2761 atcccatttc ttctcgcgga cgtgggcatc agccttggga acatggcggg acagagccag
     2821 catcagggcc atggtctgct cggccgtact cacggtgttg gcatccggcg tgttcatcac
     2881 caggataccg gccctcgtgg ccgcctggag atccacgtta tcaacaccga cgcccgcgcg
     2941 tgcgatcacc cgcagcttgc ccgggttggc caagactttg gcggtgatct tgacgcccga
     3001 gcggatgatc atgccgtcgt actcgccgac gatggcggcg agttcatctt ccttcagccc
     3061 ggtcttgacg tcggcttcga tgccgctggt ttcctggatc ttcttcatgc cttccttcgc
     3121 gagaggatcg gcaacaagga tcttgatcat gtaagtgtca cctctttcgt tcatttcgtt
     3181 cgagacaaga accgtgtcag gcgagcattc tagcgagatt cccgtccgcg tcacgtgccg
     3241 gcccccttgt gccccaaagg cgaccgatta ccatttgttc actcggagag attgacgccg
     3301 gagcagcaag gagacgaagt gaacctgaac gcagagcctg tgaaagtcgc tggcggcttc
     3361 aagttttgcg aggggccggc attcgatccg aaaggccgcc tgtgggtcgt caatcttcac
     3421 ggtggcttta tctccagagt caccatgagc ggtaagaaaa cccgggtggc cgagacgccg
     3481 ggtcccaacg gcgggcaatt cgacgccgac ggccattaca tctgctgtga gtgcaagcgg
     3541 aaggcgattg tccgggtttc acctgagggc attatttcag cccttactga atcttatgag
     3601 ggtcacccgt ttaatgggcc caatgatgtg gccattgacg ccgacggcgg cttctatttc
     3661 acggacccgg atggcagctc gctcgaaaac cggatcgggg cggtctacta cgtccggccc
     3721 gaccggacca ttctgcaggt cgactccggc ctcgcgtatc cgaacgggat caacatcacg
     3781 gtcgaccgct cggccgtcct cgtggccgag acgctgacac accgggtcta ccgctaccgg
     3841 cggcggcctg acggaacgct cgggagtcgc cacgtcttct gtcagctttc gggcggagtg
     3901 ggaccggacg gcatgtgttt tgaccaagac ggcaatctgt acgtggcctg gtatggctca
     3961 gcctgtgtct acgtggttga tccgtccggc aggccgatcg gccggctcgt gactcccgga
     4021 gacaacccga ccaactgctg tttcggtccg ccgggatcga agtacgcggc ttctctgttc
     4081 gtcacggaaa ccgtgacaaa cgcagtctgg cggtacgaca tcggcgtccc gggcatgccg
     4141 ttgcatcact tggcggtggc cagcgagtaa tcggtcgttc ttcgtcacgg gcgtgttttg
     4201 acgcgcatat ccgctccgcc gatccgcagg ccggcaggtt ttctggcgcc attcctgatc
     4261 gaacgttgag aaccaagtga tgcccggtac aatgtgccac gcttggagtc ttgtttgagc
     4321 ggacggctgt caggttgagt tcttggaact tgccggcgag ggtgtgtcag tgcctcgacg
     4381 atacgagtac ggcttcatcg gcgcgggaaa catggccgag gggatcatct ccgccgcggt
     4441 cgggaaccgg cttttcaaga gcagccggct tgcagtctat gacccggctg agcagcgaag
     4501 agaactgatg gttcagaagt tcggtgtcgc ggcggtccgg gacagctttc aattggtggc
     4561 ggaggctcaa cgcatcgtcc tcgcggtgaa gcctcagagc ttcgccgagg ccatcacgcc
     4621 gctcgcggat catgtgcggc aggatcagct catcgtcagc atcatggccg gcgtcagtac
     4681 cggtcgggtg gagtccctct ttccgcagat caaccttcgg gtggtccggg tgatgcccaa
     4741 cctgccgatt cttgtcggcg cgggcgtggc aggcgtctgt ccgggtcgtt ttgcgactgc
     4801 tcaagacgtg gccgatgtgc aggcgctgtt cgatgccggc ggggcgagcg tagtggtgaa
     4861 ggacgagtcg ctgattgacg cggtcacggc tatatcgggt tcggggccgg cctatttcta
     4921 ttatttcgtg gaagccatgg ttgccggagg ggtggcctgc gggctgagcg aggcagaggc
     4981 cctcaagctg gccgcgcaca catgcctggg agctgcgagg atgatgctgg agaccggcga
     5041 gccgccggcc gagcttcgac gcaaggtgac cagcaagggc ggcacgaccc aggcggcact
     5101 cgatgccatg aatgccgccg gagtggacaa ggccattcgc gaggccgtgc tggcagcctt
     5161 cgatcgggcg aaggagttgg gaaagaatta gcgtgacacc gaggccgtca gcttccggct
     5221 cttggctgat agtactgcgt ccataactcg ctgcgattgc gaaggagtga ccatgaagag
     5281 cgtcgccaaa acctctctga tcgggtccgt cttgttgatc ttcttggccg cgaccggctg
     5341 tgcccagccc aagtgcaagg tgcagcgatt cggggcggtg atcggcgtga agaaggaggc
     5401 catccccgag tacaagcggc tgcatgccga tacctggccc agcgttctgc aggtcattcg
     5461 cgagtgcaac atccgcaact actcgatctt tctgaaggag gtcgagaagg acaagtggta
     5521 cttgttcgcc tactacgaat acaccggcga ggatatcaag gccgacatgg agaagatgaa
     5581 gacctacaag gccatgcagg actggtggaa actgaccgac ccgttgcagg agccgctgcc
     5641 gattcgtgag aagggcgaat ggtgggctgt cggcgaagag gtattccaca cggattgaca
     5701 tgagaggcgg gggtgggaga gcaaggggcg gcgcgccgga gggccagaca cggcaattga
     5761 agattgcaga tctgagatct gagatttgag atttcagatg ccgtgagacc tgcaccgccc
     5821 cgtcgtgcag agaggcaggg gtgatctcca ggacaggtcg ggagacgttt gatgcttgcg
     5881 ttgcagttgg cgatcaaggg cctcgatctg gccattatca tcgtctactt cgtggtgatc
     5941 atcggcatcg gctgcctggc cgggctatat cgccgtcgcg cgtcggaggg taagaactac
     6001 ttcctcgccg gcgggacgct gacctggccg atcatcggcc tggcattgtt ctcgacaaac
     6061 atctcgacca tccacctggt cagccaggca caagtgggct acctcagcgg actggcctac
     6121 ggcagctttg aatggatggc cccattcacg ctgatctgcc tggcgatgtt ctttgctccc
     6181 ttctaccttc gatcgggcgt gaccacactg cccgacttcc tggagaaacg gtacagccgg
     6241 gcgagtcgca actggcttgc gcttctgtcg atgatctcgg caatattcat tcacatcggt
     6301 ttttcgctgt acaccggcgc gctggtgctc aacaagatgt tcggcattga cacgatgcag
     6361 agcgtcatcg ccatctgtct gctcaccggg ttgtacacga tcatcggcgg gctgctggcg
     6421 gtcgtgctga ccgaatcgct gcagaccatc attctgttgt gcggagccat cgccatcacc
     6481 ttgatcggcc tgacgaaggc cgggggctgg agcggcatgg cctccagcgt cgatccggtg
     6541 tatctgaacg tgatgcggac ggcggagcag ttgcccgaac tgccgtggta tgcggtgctg
     6601 ctcgggtatc cggtgatcgg gctgtggtac tggtgtgccg accagaccat cgttcagcgc
     6661 gtcctggggg ccaagaacga aaaccatgcc cgtgtcgggc cgctgttcgc cggtttcatc
     6721 aagatcctgc cggtattcat cttcgtgatg ccggggacga tcggcctggc gctgattcac
     6781 cagaacaaga tgcctgcgct gccggtggcg gcggcgcagg aagagagcgg agcggcttcg
     6841 cgacctctga cgacggcgga gcagagggtg tacgaccatc tgacgaagat cagcgcgggg
     6901 ccagacaagg ctgtcagcag agaagatctc agcaaggccg tcgatctgac caaggcggag
     6961 attgacgggg cggtggccgg cctgaccgaa ctgaaactgg tgagggacaa gtccgaggac
     7021 atctacgccc acatgattca gcacctgctt ccgacggggc tcaagggcat cgttgcggcg
     7081 gcgctgctgg cagcactgat gagcaccgtt tcgggggctc tcaactccat tgccacgctc
     7141 ttctgctacg acatctaccg gcagtggcgt ccacaggtct cggaccacaa actggtgaca
     7201 ctggggcgcg tggtgacgtt cgttgcgatg gttctgtcga ttctgtggac gccgtacatt
     7261 gcgcggtttg agaacatcta taaaggggcc aattcgctga tctgttacat tgccccgccg
     7321 atcacggtgg tgtttctgtg gggcgtattc tggcgccggg catcggcaaa gggggcgttc
     7381 gccacgctgg ttgctggttc ggtgctgggc ctgacggtat tcctgctcga atggttcaag
     7441 gactacactg gttggtcgtt gaacttcatg atggccgcgt tttatctgtt tgtcgtgtgt
     7501 tccgtggttc tggtggttgt ctcgctgctg cggcctcagc cggccgatca tcccggcaat
     7561 gccctggtgt ggaagcaccc gctcgaggct ctccagggtg aggcctggcg cgggatcggc
     7621 aactacaagt tcctggccgg gctgctgttt atcaccatgg ttgctctgta tatcatcttc
     7681 ggctgacgcg gcggaattcg gttctcgcgc cgtcagatgg gagtgaaaat gagatttgcg
     7741 gctgcattgt cggtgttggc ggcgggcttg ctggttctcg ggtgcggcaa gtccgattcg
     7801 cctgaagcgg gcggcaagac ttccggactg cggttcgcgg tgattcccaa ggggctgacc
     7861 cacgagcact ggaaggccgt ggaggccggg gcgaaaaagg ccgccaccga ggtcggcaac
     7921 gtcgaaatca tctacaaatc ggcgcccaag gaggatgaca cccagcttca gatcaacctg
     7981 gtcgagagct tcgtcagctc tcgcgtggac gggatcatcc tggcccccct gagtgatcag
     8041 gcgctggtac gccccgtgcg gctggcggtc caggcgaaga ttccggtgat catctttgac
     8101 tcgccgctgg ccgggcaggt cggcaaggat ttcgtctgct acatcgggac ggacaattac
     8161 cgggccggga ccttggccgg tcagcgactg ggcgaggtca tggagggcaa ggggactgtt
     8221 ctcttgctgc gttacgccga aagctcggcc agtacccgcg aacgtgagca gggcttcctc
     8281 gacgccctga agaaggattt cgccgacatt gccgtgatcg acccgccgca gtatgcgggg
     8341 gcggacgtca acagcggcaa gaaggccgcc gagaacatga tcactgccca cctcggcaag
     8401 ttcgagggcg tgttctgtcc gaacgaaacc agcgccgccg gcatgctgat cgccctggag
     8461 gatcgtcagc ttgcgggcaa ggctcggttc gtgggcttcg acgccaaccc ccgcgtgatc
     8521 gcgggcctga gggatcgcaa gctcgacggc gtggttctgc aggacccctt caagatgggc
     8581 tatcaaagcg tcaaacagat gctcgaccat ctcgcgggca aaccggtgcc gcccagcacc
     8641 gacaccgggg cggccgtggc gacgcctgag aacgtggaca gcccggagat ctatcggtta
     8701 ctccacccgc ttgaagctgc cgtttcgcgg ggatcgtgag ggtgacggtg ttcatggtcg
     8761 catcgcgttc cagaacggcc ttcaacgtgg gcgtcatcct cgatttctgg gccaggcaca
     8821 aatgggtgac tccggtcgtc gggctgctcg gggtggtcgt ctttttcgcc ctcaagtccg
     8881 acaattttgc ctttctcggg tccgacaaca tgcgaatcgt tgcagcccag acggtccagg
     8941 ttggggtctg cgccatcggc atgaccttca tcatgatcgg cgggggcatc gacctgagcg
     9001 tggggtcggt gatggcactg gcggcggtga ctacggcgct gaccttgaag gccggctact
     9061 cgtgcccgac ggcggtgctt gtagcgatga tgaccggcgc gttgtgcggt ctggctaatg
     9121 ccttactgat tacagggctg cgaattgtgc cctttatcgc cacgctgggg acactgagcg
     9181 ctatccgcgg cctgacgcgg cggctggccg acaaccaggt cgtccgccca ccgaacgagg
     9241 cggaaagcct ggtcagcttc gttcagcctc tgggttcatc cacttggctg gcccccgcaa
     9301 tctggatgac gttggctctt ggcgtcgcgg cggcggtggt gctcaactgg acggctttcg
     9361 gacggcggac cttcgctttg ggctcaaacg agttggccgc caggctgagc ggcatacgcg
     9421 tgaatcgtca gaaggtgtat ctgtacacgc ttggggggct cttcgcaggt ttggcgggac
     9481 tgctcgcctt tgcgaacatg cgagagggcg atccgacggc ctcggcgggc atcgagctgc
     9541 aggtgatcgc cgcggtcgtt gtgggcggcg gctcgctgat gggcggtcag ggcagcatcc
     9601 tgggcaccct ggtgggcgcg ttcatgctga ccagtttggt gaacggctgc accctcgccg
     9661 gctgggagaa ctatgtccag gaaatcgcga tcggggcgat cattgtcatg gcggtggctc
     9721 tcgattattt ccgcagacgg cgtttgaccg aaaggtgaga ctgtgaacat cggcccaatg
     9781 gtcatcgttc cgcgcggacg gtcaccagct tgaacggttt catctcgatc tggacggaac
     9841 ggtctttgac ggacagaggt gatccctgtc gttcgagcag gttgacggcc tgggctttgg
     9901 cgaagccctc aggtatctgc aggtcggcgg tttcgccgcg cccggcgtat tcgtagagcc
     9961 ggacg
//
LOCUS       DAOTPW010000216         9961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6300_length_9961_cov_5.094892, whole genome shotgun sequence.
ACCESSION   DAOTPW010000216 DAOTPW010000000
VERSION     DAOTPW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6300_length_9961_cov_5.094892"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="P5316_16910"
     CDS             <1..634
                     /locus_tag="P5316_16910"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86692.1"
                     /translation="LTASGTLITQDIYLRFLRPTANMQQVKLVTRVLEAVAMALSLSL
                     VPVAMRARSAVTFVQDFFGDVLGVVVALYIVGVFSRRATARAAFIAMITAIPLAVGLH
                     LWSDWNFAYRGFFSFAYALAATLILSRFEPPPDPARLVNLTVHTVEDVRGPWIGLKSW
                     PGLWKWAIGISVGWFAFTGIWEWYGRTQERSPTAILTSHGVYPRGSGPAG"
     gene            721..1449
                     /locus_tag="P5316_16915"
     CDS             721..1449
                     /locus_tag="P5316_16915"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRR86693.1"
                     /translation="MSDKKHILAVGAHIGDAEITAGLLISKYAAAGHRATILHMTAGE
                     KGNPRMDHLEYAKQRIREAEAAARHMGAADCIVLDHPDGQLQSDEKTIHEMCDLIRQL
                     RPDIILTHWRGSFHRDHRATYNIVMEGGFLAALPGIKRAEPAHLIRGLYYLENWEDLE
                     DYQPNLWIDVSEVFDTWVGACREHQLLRGEISFNYLHYYTGLAAKRGCEIGVKYAVTV
                     SLPPISRARKVDWLPVDTEPVLVF"
     gene            complement(1917..4124)
                     /locus_tag="P5316_16920"
     CDS             complement(1917..4124)
                     /locus_tag="P5316_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRR86694.1"
                     /translation="MDSQRSRTVGRRLVLSRVFLAVVVLDVAAVSAGQADEAAERLLA
                     GAYAVDITPTDLPVIVNGGFLERTADKVYDRLYAKCLMLAGGRERVAIVVVDSCVLPR
                     ELLDEAKELASRVTGIATDRMMISATHTHSAPSAMGCLGSDADRAYAAKLPGWIAECI
                     IGAADRLASAKIGWATVNDPEHTHCRRWIRRPDKIGLDPFGEPTVRAMMHPGPNNPDY
                     EGPSGPVDCEVSVVSVQSVDGRPLALLANYSMHYAGAPAVSADYYGQFAERMTDLLGA
                     REVKPPLVVMMSQGTSGDQWWGDYRKPLAKHDYHTIAEALSGIVLEAYRGIRYQESVS
                     LRMIERKLTLGVRQPDQKRLEAARLVVAGMQGRKPKDQKEVYAREQVFLSERPSRELK
                     LQVIRIGDLGITATPCEVFGLTGLRIKAQSPLRPTFNITLANGEEGYIPPPAQHALGG
                     YTTWPARTAQLEVDAEPKIVEALLSMLEEVGGRSRRAVDQRPDPSDARAQAVLASKPA
                     AWWPMSEFDGPTAADISGRNHHATYKGGFSFYLEGPVFEARSTNGPSNPCVHFAGGQM
                     TARVKGIADRYTLEMWFCNYMPADGRATTGHLFCRLGKEAGGKAVELLSIGGTGGASG
                     RLVFRGGEGGSQAVSGRTEIEARTWNHVVMVRDGSTVNVYLNGRAVPDIATRITAAPA
                     TGEEIMIGASPGDHDSFEGRIDEVVLYDRVLSVDELRQHWREPVRPPESEGSR"
     gene            4670..5944
                     /locus_tag="P5316_16925"
     CDS             4670..5944
                     /locus_tag="P5316_16925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86695.1"
                     /translation="MAAPQVTVIGAGMIVRIQILPTLFHMQRTGRIGEIHVCAHLPAH
                     IAELRSSPTLARAFPGQRFIAHPDPDEVPADSVHPELYREVLGAAPPNSIAVVATPDH
                     IHYPMICEALENDLHVCTVKPLVLRYDHAEDIIRRSHERGLVVGIEYHKRFDHRSLMA
                     RQAYRAGRLGEFRLGQAFLHEPYYYRDSNFQNWCTCENTDMFTYVGCHYVDLVAFITG
                     LKPVSVSVCGIRDRYPNGNQGFLWTDGRVRWDNGAILSIANAIGYPSGAAGGNAQGLT
                     MWGQGKTDATLLEHSDAYRGVIYHYTSAGTEPGDTAYAEPNPDYFQLLDTGGPGLTPV
                     GYGHRSVEYIIEACCQASQIAGLEERRSLVAQLDREGIMATPANSSYNELVIEAARKS
                     ILADGREVLIRYGSRPGVAFREYSPDQISSKE"
     gene            5954..7318
                     /locus_tag="P5316_16930"
     CDS             5954..7318
                     /locus_tag="P5316_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRR86696.1"
                     /translation="MATKEFPLQPREVELVHTKYRMIRTPIPVPDSVPFLKRLREMEP
                     RSMGGQPPVIWDGGEGATVRDPYGNRWIDFSSGVLVTSCGHAHPDIVKAIKDMADQGL
                     YHAYCFPTDVRYKLVEELTSWLPPPLKRVFLLTTGSEACECCIKLARTQGLQVGGKHK
                     NILVSFDYAFHGRTMGAQLAGGSSALKAWLGGDDPCFVQVPFPDGFRQKDTSFAVFEA
                     ALKAKGVDPNRVCGVMSETYQGCNATLMPPHYAQALRAWCDKYKAVMIFDEVQAGFGR
                     TGAPFGFSHLGIVPDLAACGKGIAGGMPVAAVLGTDELMNLYGPGEMTSTHSANPICA
                     AAALANLQVIRREKLVENAARLAPVMAEAVRRIKDASKGRIGHVDSIGLVGALQFTRP
                     GTTEPMPDWAWEVVWNCVCKGVLLFAPVGVGGCAIKLNPPLLIDEEALREGLNVVEEV
                     VRSC"
     gene            7425..9125
                     /locus_tag="P5316_16935"
     CDS             7425..9125
                     /locus_tag="P5316_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRR86697.1"
                     /translation="MNGDPIAQAASSDAFRRHLTDLLVKICAIDTVPRADVAATARAE
                     SRVFDILEQEVTHYGLKSLRCLRQPIDPVIARHPFFSQPYYTRTPENPAGLPVEQCYA
                     GRCNLVIEIDGERREPKGVGQALNAHIDVVAPYFPPRVEGNVVFGRGACDDKGSVVAI
                     VGAIKLLAEHLRATGSYLNRDLTCMIVIDEEPGGNGSLSLAVDRNLKRRYDSMMVLEC
                     ADDGIYPGNRGCVWFKIEGKLPGSYLFEAALFIIDELEKEGRAIRAESNHPLFPHRPV
                     QTCHGIIGNCGEHPSRINGDVSFEIVFESGAFATGVAGLIRDLIEDGLRQYIGLYGDK
                     TQVIDPATGKPKVDRHYDLTPTAQGWLVRVWGSTGHMGSILENDGAITKMATIGRALI
                     RSRAAIERAIGGAMRLRLSGWPDESRILMEGGQGFLPTHSMSDVQERLRAAAVRGGQY
                     YLDLVGAKVDAKHVLQVTFEKLHNAAFDGDPNSTDMLNAIEAARRAGTWKEGPIRGWD
                     VSCDARIFACEYPGLPVITTGPGLIRHAHSDQEQIDMRDVARTSEFLAYFILKQTGTL
                     "
     gene            9156..>9961
                     /locus_tag="P5316_16940"
     CDS             9156..>9961
                     /locus_tag="P5316_16940"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86698.1"
                     /translation="MDRLRLGVIGTGSVVREIYRHLYFHSDYSHLLSIEAVADPNDKA
                     LNDFADQYGIPANRRFADYHEMIRHVQLDAVQVNTPDQLHREPTIFALENDLDVMVAK
                     PLASSIADAHAIIEAARKAGRLVTVDFHKREDPRIKEVATRYHSGAYGRFQLAVLYML
                     DKLCVADPNYRPQYFASPDFCERNTPITFLTVHMADALVQMVSLKPISVRATAYSQKL
                     PSLRPVPSKGYDMCDVEVLLENGGVAHIVTCWHLPNSAHAMTVQSSRLICT"
BASE COUNT         1979 a         3412 c         2810 g         1760 t
ORIGIN      
        1 cctcaccgcg tccggcaccc tgattaccca ggacatctac ctgcgattcc tgcgacccac
       61 cgcgaacatg cagcaggtca agctggtcac ccgagtgctg gaagccgtcg caatggcgct
      121 ctcactgagt ctcgtccccg tcgccatgcg ggcacgatcg gccgtcacct tcgtccagga
      181 cttcttcggt gacgtgctcg gcgtcgtcgt ggcgctctac atcgtgggcg tcttctcccg
      241 cagagccacg gccagggccg cattcatcgc catgatcacg gcaatcccgc tggccgtggg
      301 gctgcatctc tggagtgact ggaacttcgc ttatcgcggt ttcttctcgt tcgcatacgc
      361 gctcgctgca acgctgatcc tcagccgctt cgagccgccg cccgaccccg cacgcctggt
      421 caatctgact gtccataccg tcgaagatgt gcgcggcccg tggatcgggc tcaagtcgtg
      481 gccggggctc tggaaatggg ccatcggcat cagcgtcggc tggtttgcgt tcacgggtat
      541 ctgggagtgg tacgggcgga cgcaggagcg gtctccgacc gcgatcttga cgagccacgg
      601 ggtttacccc cgtggttccg gaccggcggg ataaacctgc cggctcgtca agaccgcgaa
      661 ccgggctcgg agagccctcc tgcgaaggcg ggggagcaaa ttcggaccgg agataccatc
      721 atgagcgaca agaaacacat tctggcggtc ggggcacata tcggcgatgc ggagatcacc
      781 gccgggctgc tcatcagcaa gtacgcggcc gccgggcacc gcgcgaccat tctgcacatg
      841 accgccggcg aaaagggtaa cccgcggatg gaccacctcg aatatgccaa acagcgaatc
      901 cgcgaagccg aggccgccgc ccgccatatg ggcgccgccg actgcatcgt gctcgaccat
      961 cccgatgggc aactgcaatc cgacgaaaaa accatccacg aaatgtgcga tctcatccgg
     1021 cagctcaggc cggatatcat cctgactcac tggcggggtt ccttccatcg cgaccaccgc
     1081 gcaacctaca acatcgtcat ggaaggcggc tttctcgccg ccctgccggg catcaagcga
     1141 gccgagccgg cacatctgat ccgcggcctg tactacctgg aaaactggga ggacctggag
     1201 gactaccagc cgaacctctg gattgacgtg tccgaggtat tcgacacctg ggtcggcgcc
     1261 tgccgcgagc accagttgct ccgcggcgag atctccttca actatctgca ctactacacc
     1321 ggcctggccg ccaaacgcgg ctgcgaaatc ggcgtcaaat acgcggtcac cgtcagcctg
     1381 ccacccatct cccgcgccag gaaggtcgac tggctgcccg tcgacaccga gcccgtactg
     1441 gtgttctgat ctcgcccaga ccgcaccagg cgcccagact ccgtatcatc gaacgggggt
     1501 gggccactct tctcctccgg atcgccccac gtgttatcca cccctctgct gtcggcgctc
     1561 aggcgcgccg tgtggtcacg acggtgcttc tcttccggaa caccccgcgt gttatcctcc
     1621 cgtccgggca cctggacacg ccgcgcgctc tttgaaaacc gaagactgac ccgttcgctc
     1681 cgccttcatc actcgcgccc ctgctcccac tccacgccga gctccaaacc cgtcgaggtg
     1741 cgaagcgcca aaggaaccca atgttaaaat agagaaccct cgcttattcg agtacccacc
     1801 ctgcagtctc cgcccagcgc tgcccgcaga gcgaaaagaa cccaaactta ggaaagctgg
     1861 gaaaaccagg caagataatc gataccgaag cgagacttgg cggcaacacc atcgacctat
     1921 ctcgaaccct ccgattccgg tggccgaact ggctcacgcc aatgctgccg gagctcatca
     1981 accgacagaa cacgatcgta gagaaccacc tcgtcaatcc ggccttcgaa gctgtcatga
     2041 tcgcccgggc tggcccctat catgatctcc tcgccggtcg ctggcgcggc ggtgatccgc
     2101 gtcgcgatat ccggaacggc cctgccgttc aggtacacgt tcaccgtgct gccgtcccgc
     2161 accatcacaa catggttcca ggtccgcgct tcgatttccg tccttccgct caccgcctgc
     2221 gagcccccct caccgccccg aaagaccagc cgccccgacg cgcctccggt gccgccgatc
     2281 gacagaagct cgaccgcctt gccacccgcc tccttcccca aacgacagaa aaggtgcccc
     2341 gtcgtcgccc gcccatccgc aggcatgtag ttgcagaacc acatctcaag cgtgtagcga
     2401 tcggcgatcc ccttcacccg ggccgtcatc tgaccgcccg caaaatggac gcacggattc
     2461 gatgggccgt tggttgaccg ggcctcaaaa acaggccctt ctaaatagaa tgaaaaacca
     2521 cctttatatg ttgcatggtg attgcgaccc gagatgtcgg ccgccgttgg cccgtcaaac
     2581 tcgctcatcg gccaccaggc cgccggcttc gaggccagga ccgcctgcgc acgggcatcc
     2641 gacgggtccg gccgctggtc aacggcccgc cgagaccgac ccccgacttc ctccagcatc
     2701 gacaacagcg cttccacgat cttcggctcg gcgtcaacct cgagctgcgc cgtccgcgcc
     2761 ggccaggtcg tgtaaccgcc cagcgcgtgc tgcgccggcg gcggaatgta accctcctcg
     2821 ccgttcgcca gcgtgatgtt gaaggtcggc cgcagaggac tttgagcctt gattcttaaa
     2881 cccgtcaggc cgaacacctc gcacggagtc gcggtgattc ccagatcgcc gatcctgatc
     2941 acttgaagct tcaactcgcg gctgggacgc tccgacagga atacctgctc gcgagcgtag
     3001 acctccttct gatccttcgg cttgcgaccc tgcattccgg ccacaacgag cctggcggcc
     3061 tccagccgtt tctgatccgg ctgacgaacc cccagagtca gctttctctc gatcatcctc
     3121 agtgagaccg actcctgata acgaatgccg cgataggcct ctaacacaat ccccgaaagc
     3181 gcttcggcaa tcgtgtgata atcgtgcttc gccagcggct tgcggtaatc cccccaccat
     3241 tgatcgccgc tggtgccctg cgacatcatc accaccagcg gcggcttgac ctcgcgtgcc
     3301 ccaagcaggt cggtcatcct ctcggcaaac tggccgtaat agtccgccga aaccgccggc
     3361 gctcccgcgt agtgcatcga gtaattcgcc agcaatgcca acggccgacc gtcgaccgat
     3421 tgcacggaca ccacactgac ttcacaatcc accggcccgg acggcccttc atagtcggga
     3481 ttgttcggcc ccgggtgcat catcgcccga acggtcggct cgccgaatgg atccagcccg
     3541 atcttgtccg gccgtcggat ccaccggcga cagtgcgtgt gctccggatc attgaccgtc
     3601 gcccagccga tcttcgccga tgcaagcctg tcggcggccc cgatgatgca ctcggcgatc
     3661 caccccggca gcttcgccgc ataagccctg tccgcgtcgc tccccaagca acccatggcc
     3721 gacggcgcac tgtgcgtgtg cgtcgccgag atcatcatcc ggtcggtcgc gatgccggtc
     3781 accctggacg caagttcctt cgcctcgtca agcagctcgc gaggtagcac acaactgtcc
     3841 acgaccacaa tggccactcg ctccctcccg ccggccagca tcaggcattt ggcataaagg
     3901 cggtcgtaca ccttgtccgc cgttcgctcc aggaaaccgc cgttgacgat gaccggcaga
     3961 tccgtgggag tgatgtcaac ggcatacgcc ccggccagaa gcctctcggc cgcttcgtcg
     4021 gcctggccgg cggacacagc ggcgacatcc agaaccacca ccgccagaaa aacgcggctc
     4081 agcacgagcc tccgaccgac agtccggctt ctctgcgaat ccatgcgata cacctccacc
     4141 agcgaacgac catcgtatca aatgacccga caaatcgaca ggccatcccc ctgacggaag
     4201 acgcgcccat cgaaaaatgg tatgcgattg atcgccggac tccctgaggt ccgactcaca
     4261 gcaacttctc ctcccggcag caaagccgat caaggcggcc aacgggcgca gccgcctggc
     4321 tcgccgggca cgccgcttcc tttgggcaaa ggccttcctc agacgcctcc ggaaacgttg
     4381 acaattccgg ccccggaatc ctattatctt ggccggatca tgcccgtcga caggtctgtc
     4441 cgcaacctgc cgacgaagaa ccacaggcaa tagaagcctg ccgagaacct gatctgcccc
     4501 actggattcc cgcagtcctg aaaggtttga tcgacggcat gacggggctg gaacggtccg
     4561 gccggcgcaa aaagacaagc gggctccgcg caaccggaaa cctgttgcat ccccaaggca
     4621 acggatggca caacgccggc aagcgcgagg gctgggcgag ataaccgcaa tggctgcacc
     4681 gcaagtcacc gtcatcgggg caggcatgat cgtcaggatc caaatcctgc cgacgctgtt
     4741 ccacatgcag cgaacggggc gcatcggcga aatccacgtc tgcgctcacc ttcccgccca
     4801 catcgccgaa ctgagatcat cgccgacact cgcccgagcg tttccgggcc agcgattcat
     4861 cgcccatccc gacccggacg aagtgccggc cgattcagtc cacccggaac tctatcgaga
     4921 agtgctcggg gcggccccgc ccaacagcat cgccgtcgtc gcaacacccg accacatcca
     4981 ttacccgatg atctgcgaag cccttgaaaa cgatctccac gtctgcacgg tcaaaccgct
     5041 ggtccttcgg tacgatcatg ccgaggacat cattcgccga tcgcacgaac gcggcctggt
     5101 cgtcggaatc gaataccaca agcgatttga tcaccgcagc ctgatggcca ggcaggctta
     5161 ccgcgccggc cgactcggtg agttccggct cggccaggcg tttctgcacg agccctacta
     5221 ctaccgcgac tccaacttcc agaactggtg tacctgcgaa aacaccgaca tgttcactta
     5281 cgtgggctgc cactacgtgg acctcgtcgc gttcatcacc ggactcaagc ccgtgtcggt
     5341 cagcgtgtgt ggcatccgcg atcgctatcc caacggcaac cagggcttcc tctggaccga
     5401 cggccgcgtc cgctgggaca acggcgccat cctcagcatc gccaacgcca tcggctatcc
     5461 atccggcgcc gcgggcggca acgcccaggg gctgacaatg tggggtcaag gcaaaaccga
     5521 cgcgacactc ttggagcatt ccgacgccta ccgcggtgtg atctatcact acacctccgc
     5581 cggcactgag cccggcgaca cagcctatgc cgagccgaac cccgactact tccagttgct
     5641 cgataccggc ggaccgggcc ttacccccgt cggctacggc caccgcagcg tcgagtacat
     5701 catcgaggcc tgctgccagg cttcgcagat cgccggcctg gaggaacgcc gatcactcgt
     5761 cgcacaactt gatcgcgaag gaatcatggc cacccccgcc aacagcagtt acaacgaatt
     5821 ggtcatcgaa gcggcacgca agagcatcct tgccgacggc cgcgaggtcc tgatccgata
     5881 cggcagcaga cccggcgtcg cgtttcgtga gtactcaccc gatcagattt catcgaagga
     5941 gtaatccgta gaaatggcaa caaaggaatt tccccttcag ccgcgtgagg ttgagcttgt
     6001 tcatacgaaa taccgcatga tccgcacgcc gatcccggtt cccgactccg tgccttttct
     6061 gaaacgcctg cgtgagatgg aacctcgcag catgggtggc cagcctccgg tcatctggga
     6121 cggcggcgag ggtgccacgg tccgcgaccc ctacggcaat cgttggatcg atttctcctc
     6181 cggcgtattg gtgacctcat gcggccacgc ccacccggac atcgtcaaag caatcaagga
     6241 catggccgac cagggcttgt atcatgccta ctgcttccct accgacgtgc ggtacaagct
     6301 ggttgaggaa ttgaccagtt ggctgccccc gcccctcaaa cgcgtgttcc tcctgacaac
     6361 cggctcagaa gcttgtgaat gctgcatcaa gctggcccgc acgcaaggac tgcaggtggg
     6421 cggcaagcac aagaacatct tggtctcgtt tgactacgcc ttccacggcc gcacgatggg
     6481 cgcacagttg gcgggcggat cctctgccct caaagcgtgg ctcggcggcg acgatccttg
     6541 cttcgtgcag gtgcctttcc ccgacggctt ccgccagaaa gacaccagct ttgccgtctt
     6601 tgaggccgcg ttgaaggcaa agggcgtcga cccgaaccgc gtctgcggcg tgatgagcga
     6661 gacctaccaa ggctgcaacg ccaccctgat gccgcctcat tacgcacaag cactccgagc
     6721 gtggtgcgac aagtacaagg ccgtgatgat cttcgacgag gtccaggccg gtttcgggcg
     6781 aaccggtgcc cccttcggct tctcgcattt aggcatcgtg cccgatctgg ccgcttgcgg
     6841 aaagggcatt gccggtggaa tgccggttgc ggccgtgctc ggcaccgacg aactcatgaa
     6901 cctgtacggt ccgggcgaaa tgaccagcac gcactcggcc aatccgattt gtgcagcagc
     6961 cgccctggct aaccttcagg tcatccggcg agaaaaactg gttgagaatg ccgcccgact
     7021 cgcaccggtg atggccgagg ccgtccgccg gatcaaagat gcttccaagg gtcgcatcgg
     7081 ccacgtcgac tcgatcgggc tggtcggcgc gcttcagttc accaggcccg gcaccaccga
     7141 gccgatgccg gactgggcgt gggaggtcgt ctggaattgt gtgtgcaagg gtgtgctctt
     7201 gttcgcgccg gtcggcgtcg ggggttgcgc catcaagctc aatccgcccc tgttgatcga
     7261 cgaagaggcc ttgcgcgagg gactgaacgt ggtcgaagag gtcgtgcgct cctgctgatg
     7321 ctcgatgccc cgattcctcc ggaggtgaga tgatcgaacg tgacggcctc cgccttggcg
     7381 gattcccccg caaatgaacc gggcgtcgtt accgaggtta aggaatgaac ggagacccga
     7441 tcgcacaagc cgcctcatct gatgcgtttc ggcgccatct gaccgacctg ctggtcaaga
     7501 tctgtgcaat cgacaccgtt cctcgagccg acgtcgccgc aacggcccga gccgagtcgc
     7561 gggtcttcga cattctcgaa caagaagtaa cgcactatgg actgaagtct ttacgctgtc
     7621 tgaggcagcc gatcgacccc gtgatcgccc ggcacccctt cttctcccaa ccctactaca
     7681 cgcgcacgcc cgagaacccc gccggcctcc cggtcgagca atgctatgcc ggccggtgca
     7741 acctggtgat cgagatcgac ggcgagcggc gagagcccaa aggcgtcgga caggcactca
     7801 acgctcatat cgatgtggtg gccccctact tccctccgcg tgtcgaggga aatgtggtct
     7861 tcggccgcgg ggcctgcgac gacaaaggca gcgtggtggc catcgtcggg gcgatcaagc
     7921 tgcttgccga gcacctgcgg gcaacgggca gttacctcaa ccgcgacctg acctgcatga
     7981 tcgtcatcga tgaggaaccc ggcggcaacg gctcgttgtc cctggcggtc gaccgcaatc
     8041 tgaaacgccg ttacgacagc atgatggtgc ttgaatgcgc cgacgacggg atctatccgg
     8101 gcaaccgcgg ctgcgtctgg ttcaagatcg aaggcaagct tcctggctca tacctgttcg
     8161 aggccgcact tttcatcatc gacgaactgg agaaggaagg ccgggccatc agggccgagt
     8221 caaatcaccc gctctttccg catcgccccg tccagacctg ccacggcatc atcggcaact
     8281 gcggtgagca cccgtcgagg atcaacggcg acgtcagttt cgagatcgtc ttcgaatccg
     8341 gcgcctttgc gaccggcgta gccggcctca ttcgtgacct catcgaggac ggcttgaggc
     8401 aatacatcgg cctctacggc gacaagaccc aggtcatcga tcccgcaacc ggcaagccca
     8461 aagtggaccg ccactatgat ttgaccccta cagctcaagg ctggctcgtt cgcgtctggg
     8521 gttcgacggg tcacatgggg tcgatccttg aaaacgacgg agcaatcacc aagatggcaa
     8581 cgatcgggcg cgcgctgatc cgcagccgcg cggccatcga acgggcaatc ggtggggcca
     8641 tgcggctccg actcagcggc tggcccgacg agtcgcgaat cctgatggaa ggcggacagg
     8701 gctttttgcc cacgcactcg atgagcgatg tgcaagagcg gctccgggca gcggcggtgc
     8761 gcggcgggca gtactacctg gatctcgtcg gcgcaaaggt cgacgcgaag cacgtcctgc
     8821 aggtgacgtt tgagaaactg cacaacgctg cctttgacgg cgatccgaac tcgacggaca
     8881 tgttgaacgc catcgaagcg gcaagaaggg cagggacttg gaaggaaggc cccattcgcg
     8941 gatgggatgt gtcctgcgat gcccgtattt tcgcgtgcga gtatccgggc ttgccggtaa
     9001 tcaccaccgg gccgggtctg atccggcacg cgcacagcga ccaagaacag attgacatga
     9061 gggacgtggc caggaccagc gagtttctgg catatttcat tctgaagcag acgggcacac
     9121 tgtgagcagc ccgtcgagat gatggagaca gcgacatgga cagacttcgc ctgggagtca
     9181 ttggcacagg ctcagtcgta cgagagatct accggcatct ttactttcac agcgactatt
     9241 cacatctgct ctcgatcgag gccgtggccg accccaacga taaagccctg aacgacttcg
     9301 ccgatcagta cggcattccc gcgaatcgac ggttcgccga ctatcatgaa atgatccggc
     9361 acgtacaact cgatgccgtc caggtcaaca cccctgacca gctccaccgc gagccgacga
     9421 tcttcgccct ggaaaacgat ttggacgtca tggtggccaa gccgctggct tcatcaattg
     9481 ctgatgccca tgccatcatc gaggccgcac gcaaagccgg ccgcctcgtc accgtcgatt
     9541 tccacaagcg agaagaccca cggatcaagg aggtcgccac gcgctaccac agcggagcct
     9601 acggcagatt tcagctcgcc gttctgtaca tgctcgataa gttgtgcgtg gccgatccga
     9661 attaccgccc gcaatacttc gcctcgcccg acttctgcga aaggaatacg cccatcacct
     9721 tcctgaccgt gcacatggct gacgctctgg tacaaatggt aagcctcaag ccgatcagcg
     9781 tccgggcaac agcctactct cagaaactcc cctctctccg ccccgtgccc tccaaagggt
     9841 acgacatgtg cgacgtcgag gtactgctcg aaaacggcgg cgtggcccac atcgtgacct
     9901 gctggcacct tccgaactcc gcccacgcca tgaccgtgca gtcgtcacgg ctgatctgca
     9961 c
//
LOCUS       DAOTPW010000217         9890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6350_length_9890_cov_5.021454, whole genome shotgun sequence.
ACCESSION   DAOTPW010000217 DAOTPW010000000
VERSION     DAOTPW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6350_length_9890_cov_5.021454"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1587)
                     /locus_tag="P5316_16945"
     CDS             complement(202..1587)
                     /locus_tag="P5316_16945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRR86699.1"
                     /translation="MSVLVRRSVTGTLFSMAFPMLAGTFAMNAYNLTDTWFIAQLGTL
                     PLAAMGFVFPVVMLLTFVAGGIATGITTLVSHAIGRHDHDDAARLVTHGLALTISVAA
                     TMSVGGYLSITPIFRRLGADTQTLPLIGEYMRTWYLGAMFMALPFLGNGILISTGDSK
                     SASRFMILGTVLNLMLDPIMIFGYLGFPAMGIRGAALATVIAQAISTVWLLHLLRHRH
                     RLLAFKAGRLSEWFASFRRILGFAVPSILSMILMPISAAVITRLISHFGNEPVAACGA
                     AGRLEMFAFVVPMALGISLTPFVSQNFGAARVDRVREAQKVTTWFALGYGGLVAVVFF
                     LSAPWLASVFTDDAKVARVLISYIRIISFGYGMMEVHRYCGFFLTGLHKPVCATVLNA
                     IRVLGLLIPLSYLGAHLYGVIGVFAGRLATDLIVGSIGIIWVSRTLRSFRTNVNPTTE
                     PATARAEIVLQ"
     gene            complement(2043..3269)
                     /locus_tag="P5316_16950"
     CDS             complement(2043..3269)
                     /locus_tag="P5316_16950"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR86700.1"
                     /translation="MTAVVWIESFRIAFREMRRHRTRSILTMLGVVIGVAAIIAVVSI
                     SQGAKHSIQGQIAKVGSNMILIIPGSTSQGGVHGGAGSAKTLTIEDAEALQRECSAVA
                     YAAPVSRLVCQVVSELGNWSTPVSGTNADYLRVRSWGVQAGRPMEPRDVVSGAKVCLI
                     GKTTAQNLFGQEDPVGQRVRIKGTPMQIVGVLEEKGQNPLGYDEDDSIIVPITTCFRH
                     LAGDNRVHALVVSAVGEKEIPMAINQIRTVLRHRHQLREADLDDFTVKSISEAAKTAE
                     KASNIMTGLLVTIAGISLLVGGIGIMNIMLVTVMERTREIGVRMALGAARRMIMHQFM
                     IEAATLAGVGGALGTILGVLGSSFISNKTGIPAMMTPWLIIGPMTFAIIVGVVFGLLP
                     ARRASRLNPVESLRHE"
     gene            complement(3266..4051)
                     /locus_tag="P5316_16955"
     CDS             complement(3266..4051)
                     /locus_tag="P5316_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR86701.1"
                     /translation="MMKPIVELVDVHKVYDMGEVKVHALCGVNLTIAEGEFTAIMGAS
                     GSGKSTMLNVLGCLDQPTRGRYILDGQPVDDLDEEELARCRRMKLGFVFQNFNLLHRS
                     TALENIELPLVYLGIGVRERYRRAKEVLEVVGLADRINHMPWQLSGGQQQRVALARAL
                     VSNPRLLLADEPTGNLDSRTAAEVLELIARLHREESLTVILVTHDAEIASRAHRIVML
                     HDGAVVHDQPSPLCGGPPIPAIKSLAQVSCGGPASDENAGGRP"
     gene            complement(4059..5363)
                     /locus_tag="P5316_16960"
     CDS             complement(4059..5363)
                     /locus_tag="P5316_16960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR86702.1"
                     /translation="MARWIVRLCAIAGLGAIVWLTYRQFRPREYVQIRTVEVSRGPLC
                     LTVTATGRLAPTTQVLVGCEVSGTVGEIFVNHNDRVKKGQVIARLKPELFQAEHEQAN
                     AELARAQATLRQLEVQEAEAKRRFERIQRLQAQNAAGDEEYRTLEAAWKAATASTEAG
                     RAAVRGAQNAVDLTKYRLGKTEVISPIDGIVLDRRVDVGQTVAAALMTPELFVLAEDL
                     SRMDLLADVSEADVGYICPGQQATFTVNAYRDREFSGTVRQIRNQPHTVGNVVTYAVV
                     IEVANNELLLRPGMPADISLEVARQDDALKVPNAALRFLPPIPPDEMRRLKEKADWPP
                     TPEPIPVAGDLRATSRPDEVSLMPPPIQPGQGFLWRHVNGAWTMVPVWTGYSDNRETI
                     LYPRQGVEEGMTFVAEYIVSRSDAQSEFQKAILMTAPENRRF"
     gene            5654..6781
                     /locus_tag="P5316_16965"
     CDS             5654..6781
                     /locus_tag="P5316_16965"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HRR86703.1"
                     /translation="MSADFHRVSIVGTGSFLPGDPVPNDRLDEILGPLDHAPENVKSY
                     IQNVASRMLASSGVEFRHFAIDPKTHRLTHTVASLAEEAARRALQAAGKQPDDVELLL
                     LSCPSYDCSTPPTSAILQERLGIKQCAEMEIHSNCAGVGKCMQIACDALRCGRYKTAL
                     VVYSQLSSVYLRNCYYNQSRINKKQASLRYILADGSGAVLLQAMAPGSNHVQHELLGT
                     YVESVGGGMPAGMTAGGGVADLVDVEHQIPHVFELGTHHLDQDFSAVNRSAGKLLFEG
                     LVRMTRALGIDPHKIDAIVASIPTRQLYEDNIDLFTEYFHITREQLRFRAGKTGYCGG
                     TSLLLHFDEMVRSGEIKPGQTILLDSVESSKWMTAGFAVRW"
     gene            6783..7352
                     /locus_tag="P5316_16970"
     CDS             6783..7352
                     /locus_tag="P5316_16970"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRR86704.1"
                     /translation="MPQRNGHTDQPPPARSLSTFLESVPVTIRYVFYTVVFLSLLLVG
                     LPWLAYQIDVYVPSLHIEIGRPGRMIGVVVFVVFLIAYLLSSRLLTSRGRGGDVEFDP
                     PREFVACGIYRWVRNPIAGSAVLMLLGESLIFSSTGILLLFLLAIIVAHLQVTLLEEP
                     LLRKRFGTSYTAYLDRVPRWIPRRPRGSD"
     gene            7352..8398
                     /locus_tag="P5316_16975"
     CDS             7352..8398
                     /locus_tag="P5316_16975"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRR86705.1"
                     /translation="MQAAVTGGTGFLGEAVVRLLVPQAECVRVLVRRPEDDARVEALH
                     ARPIRGDLTDAEACAALVHPGDVVFHCAARVEMTGKWEDFRQTTIEGTRRLLHAALSR
                     RVQRFVYVSSAAVYSKENTAGGSVSAEKTRAVPARYNLYGRAKVLAESLVRTECERAG
                     CSWVILRPVFIYGPGNRVLVRNFSRLLARKRLFVVGPGTNRISTAYIDESAEAVVLAG
                     LHAKAHGRVYDVASDEVVTQAEFLNSTADALGMPRPTRHIPARLAFTAAWAADMAARL
                     PGVEPPFTRAMIDLMSTDQVVDAGRLREELGWVHRTRFAEGMRRMRDWYRAMRDKETS
                     RDSAAYRDSVLQSA"
     gene            8395..>9890
                     /locus_tag="P5316_16980"
     CDS             8395..>9890
                     /locus_tag="P5316_16980"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR86706.1"
                     /translation="MTSRPPNILLIVADCGRSDRWLGAGRSGQTPNVDRLVREGISFP
                     NTIAEKSCTTPNFAALLTGLYSPRHGVHLVWGYRLSERIPLLSHVLAAEGYHCYAEVT
                     GPLLSEMGLDRAFEVYNYRAPCDYLHTKWGSLFLERLRTGFYRQPWFMVLHLWELHPK
                     RQVQAEFNNERFGVAEYDRAVSSLDAQLGRIFAAVEDNALIAFTGDHGEKLDTETYRE
                     GTAVDYARRLLHVDEAEGMVPFSVARWAGPSVLQQFYEHCVPLMRSLRLRGNGDTPAY
                     GRWERLKDRLRLLRLTPLVFVHDLLKLRAPVKLTEVLKRRGLLDENRARARVNRFVHS
                     VGEDRLFGMHMRLWASSYKQNMQEGHTISLYDYVVRVPLVIRAPAGVPRGRSWNRMVR
                     QPDIMPTIMDFVGVDTEKLGNIDGRSFKPLVEGRPWECLPAYLSLTGLPEDLEVRGVR
                     TEQHKYTYGPHNPEMPTELYDLQSDPGETINLADHEPAKCRELRQLTE"
BASE COUNT         1849 a         3042 c         3089 g         1910 t
ORIGIN      
        1 ggattccggg ctgagcggag atgggccgac gggggcgtcg gccctacccc ccgcagccta
       61 cgcatggccg tggggatggc gcccggcaac gtcggcgatt tcaggcggtc gttctgacaa
      121 agaaagattc gctaagggag ctttctgcgc ttgacaagac gggggctaat gggtgacacg
      181 ttttcctgtc gttttcttgt cttactgcag tactatctcc gcgcgggccg tggccggttc
      241 cgtcgtcggg ttcacattcg tccggaagga acgcaaggtg cgcgataccc agatgatgcc
      301 gatgctgccg acgatcaggt ccgtcgccag gcggccggcg aacacaccga tcaccccgta
      361 gaggtgtgcc ccgaggtagg agagcgggat cagcagcccc agcacgcgga tggcattcag
      421 caccgtcgca cagacgggtt tgtgcaggcc cgtcaaaaag aaaccgcaat accgatgcac
      481 ttccatcatc ccataaccga aggagataat acgaatgtag gagataagga cgcgggccac
      541 cttcgcgtca tccgtgaaga ccgacgccag ccatggggca ctcaggaaga aaaccaccgc
      601 caccagcccg ccgtagccga gagcgaacca cgtggtgacc ttctgcgcct cgcgaacgcg
      661 gtccacccga gcggccccga agttctggct cacgaacggt gtgagcgaaa tgcccagcgc
      721 catcggaacc acgaaggcga acatctcgag gcggccggcg gcaccgcagg ccgccaccgg
      781 ctcgttgccg aagtggctga tcaacctggt gatcaccgca gcggagatcg gcatcaggat
      841 catgctcagg atgcttggca ccgcaaagcc gaggatccgc cggaacgagg caaaccattc
      901 actcagccgg ccggccttga acgccagcag acggtgcctg tgccgcagca gatgaagaag
      961 ccataccgtc gaaatcgcct gcgcgatcac ggtggccaac gccgccccgc gtatgcccat
     1021 cgccgggaag cccaggtaac cgaagatcat gatcgggtcg agcattaagt tgagcaccgt
     1081 tcccaaaatc atgaatcggc tggccgactt ggagtcgccc gtggagatca ggatgccgtt
     1141 gcccagaaac ggcagggcca tgaacatcgc tcccagatac caggtgcgca tgtattcgcc
     1201 gataaggggt agcgtctggg tatccgcacc cagacgtctg aagatcggcg tgatgctcag
     1261 atacccgcca acggacatcg ttgccgccac gctgatcgtc agggccaaac cgtgcgtgac
     1321 gagcctggcg gcgtcgtcgt gatcgtgccg accgatagcg tgggatacca gagtggtgat
     1381 gcccgtcgcg attccgccgg ccacgaacgt cagcagcatg acgacgggga agacgaaccc
     1441 catcgccgcc agcgggagcg tgcccaactg ggcgatgaac cacgtgtccg tgaggttgta
     1501 cgcgttcatg gcgaaggtgc cggccagcat gggaaacgcc atcgagaaga gggtgcctgt
     1561 gacactgcga cgaaccagta ctgacatgaa cgctcacctg tgctcactct cgagtcgggc
     1621 gcctggaccg gtgtcgtcgc cgcgttcccc ccgaggattc cgttcgcctt ttgtatgggc
     1681 cttcgcggta aggagcgacg gggccgcggc atccctcggg tccaatgtgc tggccctgtc
     1741 tacgattgcc ggggccgttg cctccacggc cgtcgtgacc gtgccgggtc tgtggccttc
     1801 agacccgacc ctcaaatcct accagtatct gtgttcagaa caaggatggg acgcagctct
     1861 tttttcgaga tcgactgctg tattcacaga ccaatagacc accagatcaa atggtccgcc
     1921 ggggcggagc ctgtgcgccg cgaccccctc cggctccctc ttacgaaggg cgaacgaacg
     1981 acccctccaa ctccccttgt gaagggagcg acctgccgtg gggggcgtcg gcatcgcccg
     2041 ggttactcgt ggcggagaga ttcgacgggg ttgagccgcg aggcacggcg ggcgggcaac
     2101 aggccgaaga ccaccccgac aatgatggca aaggtcatcg gaccgatgat cagccacggc
     2161 gtcatcatcg cggggatgcc ggttttgttg gagatgaaac tgctgccgag cacgccgagg
     2221 atggtgccca gcgcgccgcc gacgccggcc agcgtcgcgg cctcgatcat gaactggtgc
     2281 atgatcatcc gtcgggcggc acccagggcc atgcggacgc cgatttcacg cgttcgctcc
     2341 atgaccgtga ccagcataat attcatgatg ccgatgccgc cgaccaacag gctgatgccg
     2401 gcgatggtga ccagcaggcc ggtcatgatg ttggacgcct tctcggccgt cttggcggcc
     2461 tcggaaatgc ttttgacggt gaaatcgtcg aggtccgcct cgcgaagctg gtgacggtgt
     2521 cgcagcacgg tcctgatctg gttgatggcc atcgggattt ccttttcgcc gacggcggag
     2581 acgaccagcg catgaacgcg gttgtcaccg gcgaggtgac ggaagcaggt ggtgatcgga
     2641 acgatgatcg agtcgtcctc gtcatatcca agggggtttt gccctttctc ttcgagcacg
     2701 ccgacaatct gcatcggcgt gcctttgatc cggacccgct ggccgaccgg gtcttcctgg
     2761 ccgaagaggt tctgggcggt ggtcttgccg ataagacaga ccttggcgcc gctgacaacg
     2821 tctcgcggct ccatcggacg accggcctgc acgccccacg atcggactct caggtagtcg
     2881 gcattggtcc cgctaaccgg ggtcgaccag tttcccaact cactgaccac ctggcagacc
     2941 agccggctga cgggggcggc ataggcgacc gccgagcatt cgcgctggag ggcctcggca
     3001 tcttcgatcg tcagggtctt ggccgagccg gccccgccat ggacgccccc ctggctggtc
     3061 gagccgggga tgatcaggat catattgctt ccgaccttgg cgatctgtcc ctggatgctg
     3121 tgcttggctc cctggctgat ggacaccacc gcgatgatcg ccgcgacgcc gatgaccact
     3181 cccagcatgg tcaggatgct ccgcgtgcga tggcggcgca tctctcggaa ggcgatccgg
     3241 aagctctcga tccagacgac tgcggtcacg gccggcctcc tgcgttttcg tctgaagcgg
     3301 ggccgccgca acttacctgc gcgaggctct taatggccgg aatcggcggt ccgccgcaca
     3361 gcggcgacgg ctggtcgtga accacggcgc cgtcgtgcag catcacgatg cgatgggccc
     3421 gcgaggcgat ctcggcatcg tgagtcacca ggatcacggt cagcgattcc tcgcgatgga
     3481 gtctggcgat cagttcgagc acctcggccg cagtcctgga atccaggttg ccggtcggtt
     3541 cgtccgccag cagcagccgc gggttgctca ccagggcccg ggccaaggcg acgcgctgct
     3601 gctgcccgcc cgaaagctgc cacggcatgt ggttgatccg gtcggccaag cccacgacct
     3661 ccaggacctc cttggcccga cgataacgtt cccgcacgcc gatgcccaga tagacgagcg
     3721 gcagttcgat gttttcgagg gccgtgctgc gatggagcag attgaagttc tgaaaaacga
     3781 acccgagctt catgcggcgg caccgggcaa gttcttcttc gtcgaggtcg tcgaccggct
     3841 gcccatcaag gatgtagcgt ccgcgtgtgg gctgatccag gcagccgaga acattgagca
     3901 tggtgctctt gcccgagccg ctggccccca taatggcggt aaactcgccc tccgctatgg
     3961 tcagattcac gccgcacaga gcgtgtacct tgacctcgcc catgtcatag accttatgga
     4021 catccaccag ttcgacaatc ggtttcatca tgctcggatc aaaaccgccg gttctccggc
     4081 gcggtcatca ggatggcttt ctgaaactca ctctgggcgt cgctcctgga aacgatgtac
     4141 tctgcgacaa aggtcatgcc ttcctcaacg ccctggcgcg ggtagaggat cgtctcgcgg
     4201 ttatcgctgt aacccgtcca caccgggacc atggtccacg ctccgttcac atgccgccac
     4261 aggaatccct gaccgggctg gatcgggggg ggcataaggc tcacctcatc gggcctgctc
     4321 gtggcccgca ggtcaccggc gaccgggatc ggttccggcg tgggcggcca atcggctttc
     4381 tctttgagtc ggcgcatctc atccggagga atcggcggca agaaccgcag ggccgcgttg
     4441 ggcaccttta aggcgtcgtc ttgtcgggca acttcaaggc tgatatcagc gggcatgccc
     4501 ggcctaagaa gcaactcgtt gttcgcaacc tcgatcacca ctgcataggt cacgacgttt
     4561 ccgacggtgt ggggttggtt gcgaatctgt cgaacggtgc cggagaactc gcgatcacga
     4621 taggcattga cagtgaaagt cgcctgctgg ccggggcaga tgtaaccgac gtccgcctcg
     4681 ctcacgtcgg ccaacaggtc cattcgcgaa agatcctccg ccagcacaaa aagctcgggc
     4741 gtcatgaggg cagcagccac cgtctggccc acgtcgaccc gacgatccaa gacaatcccg
     4801 tcgatgggtg aaataacctc agtctttcca agacggtatt tcgtcagatc gactgcgttc
     4861 tgcgcgcccc ggacggctgc gcgacctgcc tcggtgctgg ccgtggcagc tttccaggca
     4921 gcctccaggg tgcgatactc ctcgtcgccc gccgcgttct gggcctgtag tctctggatt
     4981 ctctcaaatc gacgtttggc ctcagcctct tggacttcca gttggcgtag ggtggcctgg
     5041 gcccgagcca gctcggcatt cgcctgttcg tgttccgcct ggaaaagctc tggtttcagg
     5101 cgggctataa cctgcccctt cttgacgcga tcattgtggt tcacgaagat ttcgcccacg
     5161 gttccagaga cttcgcagcc gacgagaacc tgggtcgtgg gtgccagacg cccggtcgcc
     5221 gtcactgtca gacaaagagg accgcgactg acttcgacgg tgcggatttg aacatactct
     5281 cgcggcctga actgccgata ggtcagccaa acgatggctc ccagcccggc aatcgcacac
     5341 aagcgcacga tccacctagc cataagcgac tcccaagcgt cgcggaaagc cgccggcgcc
     5401 gtgaaaccgg gaccgtgtat taccgatgct attcatgggc ttccttgccg cctctggccc
     5461 aacaaatagg ggataaaggc atgtgcgtcc ctgatccaat gccgcttttg cccgtcgctt
     5521 cccggccgtg ttctgtctcc gggcatgttg acagttgcgg acgcctgcga caatatacgc
     5581 gtcttacaac gacggctgac taaccaaggc agtagggtag catagtcaga gcacgagcga
     5641 aagagatcga tacgtgagcg ctgatttcca ccgcgtttcc attgtgggca cgggttcatt
     5701 tctcccggga gaccccgtgc cgaatgatcg acttgatgag atcctggggc cgctggacca
     5761 tgccccggaa aacgtcaagt cgtacattca gaatgtggcc tctcgtatgc tggcttcaag
     5821 cggcgtggag ttccggcatt ttgccattga ccccaagacc cacaggctga ctcatacggt
     5881 cgcctcgctg gcggaagaag ccgcaaggcg ggccctccag gccgcaggca agcaacccga
     5941 cgacgtcgaa ctgcttctgc tgagctgccc gagctacgat tgctcgacgc cgccaacctc
     6001 ggcgatactc caggaaagac tggggatcaa gcaatgcgcc gagatggaga tccactccaa
     6061 ttgtgcgggt gtgggcaagt gcatgcagat cgcctgcgac gcccttcggt gtggccgata
     6121 caagaccgct ctggtcgtct actcgcagtt gtccagtgtc tatcttcgga actgctacta
     6181 caatcagagc cggatcaaca agaagcaggc ctcgttgagg tatatcctgg ccgacggcag
     6241 cggtgccgtc ctgctccagg caatggcccc gggcagcaac cacgtgcaac acgaattgct
     6301 gggaacctat gtcgagtcag tcggcggcgg gatgcccgcg gggatgacgg cgggcggcgg
     6361 cgtcgccgat ctggtggacg tcgagcatca gatccctcac gtgtttgaac tcggtacgca
     6421 tcatctggac caggattttt ccgccgtcaa tcgcagcgcc ggcaagctgc tcttcgaggg
     6481 gctcgtgcgg atgacccggg ctctggggat cgatccgcac aagattgatg ccattgtggc
     6541 atcgattccc acccgacagc tctacgagga caacatcgat ctgttcaccg agtacttcca
     6601 tatcacccgg gaacaattgc gattccgcgc cggcaagacg ggttactgcg gcgggacatc
     6661 cctgctgctc cattttgatg agatggttcg atcgggggag attaagccgg gacagacgat
     6721 tctgctcgac tcggtcgaat ccagcaaatg gatgactgcc ggcttcgcgg tgcggtggtg
     6781 agatgccgca gcgcaacggc cacaccgatc agcctccgcc agctcgaagc ctctcgactt
     6841 tcctggaatc cgttccggtc acgatccgtt acgtcttcta cacggtcgtc tttctgtcgt
     6901 tgctgcttgt tggactacct tggctggcgt atcagattga cgtgtatgtt ccttcgcttc
     6961 acatcgagat cggccgtccg ggacggatga tcggcgtcgt cgtctttgtc gttttcctga
     7021 tcgcttacct gctctcgtcg cgattgctca ccagtcgcgg gcgaggcggc gatgtggagt
     7081 tcgatcctcc gagggaattc gtcgcctgcg ggatctaccg ctgggtccgc aacccgatcg
     7141 ccggcagcgc ggttctgatg ctgctgggcg agtccctgat cttctcatcg acgggcatcc
     7201 tgctgctttt cctgctggca ataatcgtcg cccatttgca ggtcacgttg ctggaagaac
     7261 cgctgctacg caaacgcttc ggcacaagct atactgccta ccttgaccgg gttccgcgtt
     7321 ggattccacg gcgtcccaga ggatcggact aatgcaggca gccgttaccg gtggtacggg
     7381 attcctcggc gaagccgtcg ttcgccttct ggtgccgcag gcagagtgcg tccgcgtcct
     7441 cgtgcgtcga cccgaggatg acgcgagggt tgaggccctt cacgcccggc cgatccgcgg
     7501 tgatctgacc gacgcggagg cttgcgccgc cctggtccat cccggcgacg tggtttttca
     7561 ctgcgcagct cgcgtcgaga tgacgggaaa gtgggaggac ttccgacaga cgaccattga
     7621 gggcacgcgg cggttgctgc atgcggcttt gagccggcgc gttcaacgtt ttgtttacgt
     7681 cagttcggcg gcggtctatt caaaggagaa taccgccggg ggttcggtgt cggccgagaa
     7741 gacgcgggcc gttccggcgc ggtacaatct gtacggtcgt gccaaggttc tggccgagtc
     7801 gctggttcgg acggagtgtg agcgggcggg ctgttcgtgg gtgatcttgc ggcccgtatt
     7861 catctatggg ccgggcaatc gagtgctggt aaggaacttt tcgcggctgc tggcccgcaa
     7921 gcgtttgttt gtcgtcggtc ccggcaccaa ccggatttcg accgcgtata tcgacgaatc
     7981 agcggaagcc gtcgttctgg ccggcttgca tgccaaggcc cacggcaggg tatacgacgt
     8041 cgccagcgac gaggtcgtca ctcaggccga gtttctcaac agcacggctg acgcgctggg
     8101 catgcctcgc ccaaccaggc atattccggc gcgtctggcg ttcacggcgg catgggcggc
     8161 cgatatggcg gcccggctgc cgggggttga gccgcccttc acgcgggcca tgatcgactt
     8221 gatgagcacc gaccaggtcg tcgacgccgg aaggctgcgt gaagaactgg gctgggtgca
     8281 ccgcacccgt tttgcagagg gcatgaggcg aatgcgggat tggtaccgag cgatgcggga
     8341 caaggagact tccagggact ctgccgcgta ccgcgacagc gtcctgcaat cggcatgaca
     8401 agcagacccc ccaacatact gctgatcgtg gccgattgcg gtcgttccga ccgctggctc
     8461 ggggccggcc gatccggaca gacgcccaat gtggatcgcc ttgtccgaga agggatctct
     8521 ttcccaaata cgatcgccga aaagagctgt accactccca actttgcagc actcctgacg
     8581 ggcctgtaca gcccgcgaca cggcgttcac ctggtctggg gttaccggct gagcgagcgg
     8641 attccgctat tgtctcatgt cttggccgcc gaaggctatc actgctatgc ggaggtgacc
     8701 ggcccgctgc tgtcggagat gggtctggat cgtgcttttg aggtttacaa ttaccgtgcc
     8761 ccgtgcgatt acctgcacac caagtgggga agcctctttc tcgaacgcct acggaccggc
     8821 ttctatcgcc agccctggtt catggttctt cacctctggg agctgcaccc gaagaggcaa
     8881 gttcaagcgg agttcaacaa cgagcgtttc ggtgtggccg agtatgaccg ggcagtgtcg
     8941 agcctggacg cgcaactggg gcgcatcttc gccgcggtgg aggacaacgc gctcatcgcc
     9001 ttcacggggg accacggtga gaaactcgac accgagacct accgagaagg caccgcggtg
     9061 gattacgctc gccggctgct gcacgtggat gaggccgagg gcatggtgcc tttcagtgtg
     9121 gcccgttggg ccggccccag cgttctgcaa caattctacg agcactgcgt gccgctgatg
     9181 cggagcttgc ggctacgggg caacggagat actccggctt acggccggtg ggaaagactt
     9241 aaagatcgtc ttcgactact caggttgact ccgctggttt tcgtgcacga tctactcaaa
     9301 ctccgtgccc ctgtgaagct gaccgaagta ctgaaaaggc gaggcttgct cgatgagaac
     9361 cgagccaggg cccgagtgaa ccgctttgtt cactcggtcg gcgaagatcg gctgttcggc
     9421 atgcacatgc gtctgtgggc gagttcttac aagcaaaaca tgcaagaagg ccataccatc
     9481 tcgctgtatg attacgtggt gcgtgtacca ctggttatcc gcgccccggc gggggtgcct
     9541 cgcggccgat cctggaaccg gatggttcgc cagccggata ttatgccgac catcatggac
     9601 ttcgtgggcg tcgacactga gaaactgggc aacatcgacg gccgatcgtt caagccgctg
     9661 gttgaggggc gaccctggga atgcctgcct gcttatctct cgctgacggg tctgcccgaa
     9721 gatctcgagg tgcgcggcgt gcgaacggag cagcacaagt atacctatgg gccgcacaat
     9781 ccggagatgc ccacggagct ttacgatctg cagagcgacc ccggcgagac gatcaacctg
     9841 gcagaccacg aaccggccaa gtgcagggaa ctgcggcaac tcaccgagag
//
LOCUS       DAOTPW010000218         9867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6368_length_9867_cov_5.031390, whole genome shotgun sequence.
ACCESSION   DAOTPW010000218 DAOTPW010000000
VERSION     DAOTPW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6368_length_9867_cov_5.031390"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            808..4431
                     /locus_tag="P5316_16985"
     CDS             808..4431
                     /locus_tag="P5316_16985"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRR86707.1"
                     /translation="MRITIQSGLTAAMTALLLLGAAPAVGTPYFLIDSEMEWQEALGG
                     GGGAGMVMPMTEPEWSEYMAQWHMPSWVEGEPYPDTTFMPARLPTGQLYVYGGSGGGG
                     PDPEDAGLVMVWGAGTALPEGQYSSAWKYEYGLDPDLTNTVITVTVTAPRFDLKGNQI
                     NNVSFGIRDALGAIRSWQWKCGLLGPIPWNVPTTITIRPMVASVNATTPVASGFLNNP
                     MFNITQSAVFVVDENARWVGGPLPVPPPGGQIPAGLWNYWHNLVVTPIIQPKPPLTTK
                     WSQPPSQYDGPGHNPQPVFYGWDEVSLYHIGPPIVADDWRCTDPRPVTDIHWWGSFIG
                     WTQPDPPQMPDAFHIGIWTDVPTGAGELFSHPGKLIWETYCTNFTWNFAGYDYDPRGL
                     VQNEACFQFNQYLNPDEYFYQEPNPDGNIYWISISAIYHNLPPAHPWGWKTRPHFFQD
                     DAVRIWAVQDPTGLTGWPPALGWSWVQGQPIEYPQGTSWDLAFELSTIEGQVGACCYQ
                     DPAGPGLIQCLVTDQTTCEQQLAGVWKGPGTDCSDLNGNGIADICEEQPEMLEFGDAP
                     EGVLAYPASGVIGNFPTCKNVPPAPWIQHTNFGAWLGPQFDFEADGNAGLCPGFNPYD
                     ADECFQDGDAGLMFPPAYTINAVGNVALCPMSPGGGSLGQPCQTAIWGMNIDVDVHNH
                     MPNQSVGYVNVLIDWDQNGQWGGASPCPLANAPEHVLVNFRIPNPFDGPVSALMPAGT
                     GFLIGPNAGEVWMRVSITERPVPLNWDGSGAFEDGETEDYLLNVEQPKHDLGDAPDST
                     NSFGAAMTAYPPGGPPGVQAHYPTVFAAGSPPFGPIHLQPGAVAFLGNAVTLENEADM
                     GLDQDPTNNIVPLADAPDKDGADDGLLLPLVFPHCRPAQFNYVVTVTGMVATPLYVNA
                     WCDWNRDGDWDDMLTCPQGLADEWAVQNQMLAVAGPGTYTFTTPPFLPWHPVGAPGDR
                     LPLWMRITLSEQPWQPGPGIVGYGGSGPAAGYQFGETEDYFVTPEIPEPPKAVAWRSV
                     RNHGPLGPLAIDLDPSATGSSVKSETRRNGIRLIEVDFDQAVVLTNPGGITVTDWPGG
                     GSYVPTSVAMVDADTLAIGFAAGLLPDMNCYRIDLDTCVQSLAGLPLAGDTDCWVRAL
                     VGDVNSDGGVSATDVAFVKSKVGQNPALPGNARFDLNTDGNISATDMALVKTLVGNRA
                     SCP"
     gene            4753..5304
                     /locus_tag="P5316_16990"
     CDS             4753..5304
                     /locus_tag="P5316_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRR86708.1"
                     /translation="MASSLATRIVLQRGDITKVAADAIVNAANTSLLGGGGVDGAIHR
                     AAGPELLAECRKLGGCPTGSARITKGYRLPARYVIHCVGPVYHGGRSGEAQLLASCYE
                     TALRLAVENGCKTVAFPAISCGVYGYPLAEAARIALQQVASFLQDNQQIEQVVFVLFD
                     ERAYQAFDRAYCERFGPKPEMTS"
     gene            5301..6326
                     /locus_tag="P5316_16995"
     CDS             5301..6326
                     /locus_tag="P5316_16995"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRR86709.1"
                     /translation="MKLSWERRQLRLRHPFNIARMPMSDSTDKTVLLARIEHDGLVGW
                     GEAAPISFYHQSFDSAEATLSKIGDMLGNDPFALDAILDNLLKRFPGESAAICAIDGA
                     LHDLIGKILKVPLWKMFGLDRSRMPLTSMTIGIDKPEVMAAKVREAAAFPILKVKVGT
                     PDDEIILNTVRREAPNKTIRVDANCGWSSENAFERCKLMADYKVEFIEQPTRPGDNDV
                     LPRLREAGLGPLYADESCVTVKDVLFCVGFFDGINIKLSKCGGIRQAMKMIHVARAAG
                     LKIMLGCMTETSVGIAAAAQLGPMVDHLDLDGHLLLANDPFTGLGGEGGRLTLTDAPG
                     LGIRELT"
     gene            complement(6339..7505)
                     /locus_tag="P5316_17000"
     CDS             complement(6339..7505)
                     /locus_tag="P5316_17000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRR86710.1"
                     /translation="MAEYAKRDELESALRRESGVIGGIGAGLVENPAPVTAATSNERP
                     DCPQGRKVLVADDEPSTRRVLEGLLTNAGYVVFAAPNGMEALRILNDVGPQIVIADWE
                     MPFMNGLQLCHEIRHSETVGFVYVIILTSHTDRVVEAFDAGADDFLTKPPRKTELLAR
                     LKAAARIIDLEAHLARQNREIHKVNAELSLLNRRLREMAATDELTGLANRRVMMERLK
                     HCWETASRNNRPLSCMVIDVDHFKQFNDNYGHDAGDMVLKAVASTLAKHTRSSEPVFR
                     CGGEEFVLICPDTPMEKAAMMAECLRQAVGQEETRYDGLVLRATISIGVSQRDAGFRC
                     PDDLLRHADGALYAAKASGRNCVRVDGPGYDDRAVGDPASGGLTTPSPVSAQVR"
     gene            complement(7912..9333)
                     /locus_tag="P5316_17005"
     CDS             complement(7912..9333)
                     /locus_tag="P5316_17005"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86711.1"
                     /translation="MSNPLTRRQFLGNSGKTVAGVAAGLSVLGPRSMLSSAWAAGANK
                     RLGIALIGCGGMGRHKLGNFLDTKQVDVLALCDVDTEQMDKAEAVITEKLKDAAGQKK
                     PQRFKDFRKVLDMKEVEIVIIATPDHWHAAPMLLACQAEKDIYVEKPCCHNIREGKAM
                     VAAAEKYKRVVQVGTHQRSYEHIQAAREFVRNGELGEISATNTYTYGNESPDGLGNEP
                     DSDPPPTVDYNLWLGPAPERKFNKRRFHSSWRWYFDYGCGMVGDWNVHLQDIIMWTLD
                     TPHPTSVCSMGGHYILADDRDTPDTMVTIYKFPPSKLAPKGHVHTYTLRKASGKPWHK
                     GGIGMDFHGTNGFLHMTRGGWEVEPDLEDWNRPGSEPRIDPVKEMGEYHKKDVEGHSA
                     HVENFLECVQSREKPIASIDQHYTSVVACHLANVSLRVGRQIFWNHEKELCFMDEKMT
                     VEDKEANKFLGREYRKGFELPEV"
     gene            9586..>9867
                     /locus_tag="P5316_17010"
     CDS             9586..>9867
                     /locus_tag="P5316_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86712.1"
                     /translation="MYERYNSEFMSSTLDHFIQPAEGARVSFAGRYPRDYLSSDPPRR
                     LLAWHLVGGLDPLDASELTGSEPEDGYPVLLPDWIRRDGLKCLKVKLRGN"
BASE COUNT         1908 a         2961 c         2958 g         2040 t
ORIGIN      
        1 cgtcctgcgg caacaagacc ttgcgcggca aacgcacctg ggagatcctc accagcctgg
       61 ctgccacctg ctaccaaccg ggccaggact ccgtcgagta cctccggcca ttcttggccc
      121 ttgctcccgc gtcctcagca cgccaagcac gtacaccggc agggtagtct gatccgatga
      181 ttgaactcgc cgtcgttttg catgggaccg cgggtattct cgacggtcct ttggttcctg
      241 tatcacaaca tatcaggcct caacggtaag gccgatagct gtcagtcctg caaaccccct
      301 ttgccgtggc cgggtcgagg caaacgcacg ttcatcaaaa ggattgcggt tttttccttt
      361 gacaatgcat ccatggggtg ttagcctgat attgaacgct caagcgtctc aagtctgtgg
      421 caagcgcgtg aggagacgcc gtctgtggga agcgaggggc ggtgtgtggg atcaggaagt
      481 ctgagcccgc tgcacaacgt aactgcctcc cggaagacag aatgtctgga tgcagacatc
      541 gctcgcgtgg ccggcttgtt tgagagagtt ttttcccact cggggcggag ggttcgcgtg
      601 ggtagtggtc aggcagccgc gggaatatcc actggtcacc catgccgcga aaaacgattc
      661 tttcgcgtgc gcgcactccg atgcggcgag gatccgcagg cgccgagcag ggatgttgta
      721 tgcctcgcgg aggggcattc cccaaaacca gactcatctc atgatggcat gacacacagg
      781 tacgtcacat aaaaaaggag gtgtacgatg cgaatcacga tccaatcagg gttaacggca
      841 gcaatgacag ccttattact tctcggggct gccccggccg tgggtactcc ctatttcttg
      901 atcgactcgg aaatggagtg gcaggaagcc ttgggcgggg gtggtggtgc cggcatggtc
      961 atgccaatga ccgaaccgga gtggtcggag tacatggccc agtggcacat gccttcttgg
     1021 gtcgaggggg aaccctatcc cgatacgacc tttatgcccg caaggttgcc cacagggcaa
     1081 ctgtatgtgt atgggggcag cggcggtggc ggcccggacc ccgaggatgc gggtctggtc
     1141 atggtttggg gagcggggac ggcacttccg gagggccagt actccagcgc gtggaagtat
     1201 gagtacggcc tggacccgga tctgacgaac acggtcatca ccgtgacggt gactgcccca
     1261 agatttgacc tgaaaggcaa ccagatcaat aacgtttcgt tcggaattcg ggatgctttg
     1321 ggagccatcc gttcctggca gtggaagtgc gggttgctgg gcccgattcc ctggaacgtg
     1381 ccgaccacga ttaccatccg gccgatggtg gccagcgtga acgcgaccac gccggtcgcc
     1441 agcggtttcc tgaacaatcc gatgttcaac attacccagt ctgcggtttt tgttgtcgac
     1501 gaaaacgcgc gatgggttgg cgggccgctg ccggtgcccc cgcctggcgg ccagatcccc
     1561 gctgggctct ggaactactg gcacaacttg gtggtgaccc cgattattca gccgaaaccg
     1621 ccgttgacca ccaagtggag ccagccgccg tcgcagtacg atgggccggg tcataatccg
     1681 cagccggtct tctacggctg ggacgaggtc tcgctgtacc acatcggccc ccccatcgtt
     1741 gccgatgact ggcgctgcac cgacccacga cctgtgaccg acatccactg gtggggctcg
     1801 ttcatcggct ggacccagcc ggatccgccc cagatgcccg acgcgtttca catcggtatc
     1861 tggacggacg tcccgaccgg cgcgggcgaa ctcttcagtc atcccggaaa actcatttgg
     1921 gagacgtatt gcacgaactt tacgtggaat ttcgcgggtt atgactacga tccgcgaggc
     1981 ctcgtgcaaa acgaggcctg cttccagttc aatcagtacc tcaaccccga cgagtacttc
     2041 tatcaggagc ccaaccccga cggtaacatc tactggatca gcatctcggc catttatcac
     2101 aacttgccac cggctcatcc atggggctgg aagacccggc cgcatttctt ccaggatgac
     2161 gccgttcgca tctgggctgt tcaggacccg acaggtctca ctggttggcc gcctgcactg
     2221 ggctggagct gggtacaggg tcagccgatc gaatacccgc agggaacatc gtgggatctg
     2281 gctttcgagt tgtccaccat cgagggacag gtgggagcct gctgctacca ggatcctgct
     2341 ggtcccggac ttatccagtg cctcgtgacc gatcagacca cctgcgagca gcagcttgcg
     2401 ggtgtttgga aaggtcctgg caccgactgc tccgatctga atggcaacgg catcgcggat
     2461 atctgcgagg agcagcctga aatgctggag ttcggcgatg ctcccgaggg cgtcctcgcc
     2521 tatccggcca gcggcgtgat tggcaacttc ccgacctgca agaacgtccc gccggccccg
     2581 tggattcagc acaccaactt cggcgcctgg ctgggcccgc agtttgattt cgaggcggac
     2641 gggaacgcag ggctttgccc gggattcaac ccgtatgatg ccgacgagtg cttccaggac
     2701 ggcgacgccg gcctgatgtt cccgccggca tatactatca acgccgtagg gaatgttgca
     2761 ctctgcccga tgtcgccggg aggcggctcc ctcggccagc cttgtcaaac cgccatttgg
     2821 ggtatgaaca tcgacgtgga tgttcacaac catatgccca accaatcggt cggatatgtg
     2881 aacgtactga tcgactggga ccagaacgga cagtggggcg gagcgtcgcc gtgtccgcta
     2941 gccaacgcgc ccgagcacgt gctggtcaat ttccggattc cgaacccctt tgacggtccg
     3001 gtttcggctc tgatgcctgc gggcaccggc ttcctgatcg gcccgaatgc cggcgaggtc
     3061 tggatgcggg tttcgatcac cgaacggccg gtacccctga actgggatgg ttcaggcgca
     3121 ttcgaggacg gcgaaaccga ggattatctt ctgaacgtcg agcagcccaa gcacgatctt
     3181 ggcgatgctc cggacagcac caacagcttt ggcgcggcta tgaccgccta tccaccgggt
     3241 gggcctcccg gagtacaagc acactacccg actgtgtttg ccgcagggtc tccacccttc
     3301 gggccgattc atttgcagcc cggcgctgtc gccttcctgg gcaacgcggt cacgctggag
     3361 aacgaggccg atatgggtct ggaccaggat cccaccaaca acatcgttcc cttggcggac
     3421 gccccggaca aggatggtgc cgatgacggt ctgctgttgc cgctggtctt cccgcactgc
     3481 cgtccggcgc agttcaacta tgtcgtgacg gtgacaggaa tggtagcgac tccgctttat
     3541 gtaaacgcat ggtgtgactg gaatcgcgac ggcgattggg atgacatgct gacgtgcccg
     3601 cagggactgg cagatgagtg ggcggtccag aaccagatgc ttgcggtcgc aggacctggt
     3661 acttacacat tcaccacgcc cccattcctg ccgtggcatc cggttggggc tccgggtgac
     3721 aggttaccgc tgtggatgcg aatcactctg tcggagcagc cgtggcaacc aggaccgggg
     3781 atagtcggct atggtgggtc cgggcccgct gccggttatc agttcggcga gacggaggat
     3841 tacttcgtca ccccagaaat acccgaacct ccgaaggccg ttgcctggcg gagcgtccgg
     3901 aaccacggtc ctctgggacc gctggcgatt gatctcgacc cttcagcaac gggtagttcg
     3961 gtgaagtccg agactaggcg aaacgggatc cggttgattg aagtcgattt cgatcaagct
     4021 gtcgtactaa ccaatcctgg aggtattacg gtgaccgatt ggccgggagg gggctcgtac
     4081 gttccgacgt ctgtggccat ggttgatgcc gacacccttg ccattggttt tgccgcaggt
     4141 ctgcttccgg atatgaattg ctataggatt gatctggaca cctgcgtgca gagccttgcg
     4201 ggtctgccgc tggcaggtga cacggactgt tgggtcagag ccttggttgg tgatgtcaac
     4261 agcgatggtg gggtttccgc taccgatgtg gcgtttgtta agtccaaagt tggacaaaac
     4321 cctgctcttc ccggcaacgc tcggtttgac ctgaacactg acggaaatat tagcgccacc
     4381 gacatggcgc tagtgaagac attggtagga aacagagcgt cttgcccgta gcgagaacag
     4441 tcgcggcaga cgtaggtctt gagatagtgc ccgccgggac agatcctgtc ccggcgggtc
     4501 tttcttgcgc gaaatcgcgc cggcgtgcga gcaaccacgg ctgttcggtc aacttcatgt
     4561 tgaatgatgg cgtgggcggc ggttcaccgg acgcttacga tggtttggcg cgcagtgagc
     4621 ccgatgccgg aaaaacagaa caaacgtgag cgtccactga gccacatgac aaagtgccgc
     4681 tgagcaacaa gcggcggcgg gtcttgcccc cgcattgacg agccataccc tcagcaccta
     4741 tattcgtagc ccatggcaag ctcgctggcc accaggattg ttctgcagcg cggcgacatt
     4801 actaaggtcg cagcagatgc gatcgtcaat gccgccaata cctctcttct gggcggcggc
     4861 ggggtggacg gggccattca ccgggctgcc ggacctgagt tgctggccga atgccggaaa
     4921 ctcggcggtt gtcccactgg atcagccagg atcaccaagg gctaccgcct gccggcgcgg
     4981 tacgtgatcc attgtgtcgg gccggtttac cacgggggcc ggtccggcga ggcccaactc
     5041 ctggccagtt gctacgagac cgctttgcgg ctggcggtcg aaaacggatg caagaccgtt
     5101 gcttttcccg cgatcagttg cggggtctac ggatacccgc tggccgaggc cgccagaatc
     5161 gccctccagc aggtggcgtc attccttcaa gacaatcagc agatcgaaca ggtagttttc
     5221 gtcctgtttg atgagcgagc ataccaggcc ttcgaccgcg cctattgcga acgctttgga
     5281 cccaaaccgg agatgacatc gtgaaactga gctgggaacg tcgacaactc agactccgcc
     5341 accctttcaa catcgccaga atgcccatgt ccgacagcac ggacaagaca gtgcttctgg
     5401 ctcggatcga gcacgacgga ctggtaggtt ggggagaggc tgccccgatc tcattctatc
     5461 accagtcgtt cgattcggcc gaagcgactc tttcaaagat cggcgatatg ctgggcaatg
     5521 atccgttcgc tttggatgct attctggata accttctgaa gagattccca ggcgaatccg
     5581 ccgccatttg tgcgatcgac ggggctcttc acgaccttat cggcaagatt ctcaaggtgc
     5641 cgttgtggaa gatgttcggc ctggaccgct cgcggatgcc tctaacgtcg atgaccattg
     5701 ggatcgataa gccggaggtc atggcggcca aggtccgtga agctgccgcc ttcccgattc
     5761 tcaaggtcaa ggtgggcacg cccgacgacg agatcatcct caacaccgtc cgccgggagg
     5821 cacccaataa gacaattcgc gtcgacgcca actgcggttg gtcgtcagag aatgccttcg
     5881 aacgatgtaa gctgatggcg gattacaagg ttgagttcat cgagcagccg accaggcccg
     5941 gcgataacga cgttcttccg aggctgaggg aagccggcct gggaccgcta tacgccgatg
     6001 aaagctgcgt cacggtgaag gatgtcctgt tctgtgtcgg cttttttgac ggaatcaaca
     6061 tcaaactcag caagtgcggt ggaattcgcc aggcgatgaa aatgatccac gtggcccgcg
     6121 cggccgggct caagatcatg ctggggtgca tgaccgaaac gtccgtcggc atcgcggcag
     6181 cggcacaatt gggacccatg gtcgatcatc tcgacctgga cggacatctt cttctggcca
     6241 atgatccgtt cacaggcttg gggggagagg gagggcgact gacgcttacc gacgcgccgg
     6301 ggctcggcat cagggagctc acgtaggcgc acgccgttct accggacctg cgcagagacg
     6361 ggacttgggg tggtgagtcc gcctgatgcg gggtcgccga ccgctctatc gtcgtacccc
     6421 ggcccgtcaa cacgcacgca gttgcggccc gatgccttcg cggcatacaa agcgccatcc
     6481 gcatgccgga gaagatcatc cgggcagcga aagccggcat ctcgctgcga cacaccgata
     6541 ctgattgtgg ccctcaacac cagaccgtcg tacctggtct cctcttgccc caccgcttgg
     6601 cgcaggcatt ccgccatcat ggccgccttc tccattgggg tatcgggaca aatgagcacg
     6661 aattcctctc caccgcagcg gaataccggt tcgctggagc gagtgtgctt cgccagagtc
     6721 gacgccacgg ctttcaggac catgtcaccg gcatcatgac catagttgtc attgaactgc
     6781 ttgaagtgat caacatctat taccatgcag gacaacggcc ggttgttgcg agaggccgtt
     6841 tcccagcagt gtttcagacg ttccatcatg acccgacggt tcgccaggcc ggtcaattca
     6901 tcagtggcgg ccatctccct gagcctgcga ttgagcagac tcagttccgc gttgaccttg
     6961 tgaatctccc ggttttgcct tgccagatgg gcctcaagat caataatgcg agccgccgcc
     7021 ttcagacggg ccagcaactc ggtttttcga ggtggtttgg tcaggaaatc atccgccccg
     7081 gcgtcgaatg cctcgaccac gcggtcagtg tgcgacgtca gaatgatcac atacacgaaa
     7141 cccaccgtct ccgaatgtcg gatttcatgg cagagctgca gcccgttcat gaacggcatt
     7201 tcccaatcgg cgatcacgat ctgcgggccg acatcattga gaatacgtaa tgcctccatg
     7261 ccgtttgggg cggcaaacac cacgtacccg gcattggtca gaagcccctc aagcactcga
     7321 cgagtcgaag gctcatcgtc ggcgaccagc actttgcgcc cttgcgggca atcgggccgc
     7381 tcgttcgagg tggccgccgt caccggcgcc ggattctcaa cgaggcctgc tccaatccct
     7441 ccgattaccc ctgactcgcg ccggagggca gattccagtt catctctctt tgcgtattca
     7501 gccatttaaa ccgaggccgc cttcggcagc gaccagaaca tgcccgcgac ttgcgcgcat
     7561 gacgagccct cagcatccaa tccatgataa ggtcgtcaca tctgacggct tggttgagat
     7621 atgcgagatc cgtatgcagc gtccatctct tttttggacc ggcacaaggc attcgggacc
     7681 aatgcacggg tttgtcagcc tcgatcgtgt atggtcacac ggtcgggcat ctcggcgccc
     7741 tatgcctggc gagggaattg gctgacacat gacaggtgtg cccgacgaac gacctccgca
     7801 gagaacgctc atggttctcg tttagagccc agcgcatccg cttatcggac gaagccgaaa
     7861 gccgcccgcg gcacccgccc attcggggat actcgcgaac caacgggagc gctagacctc
     7921 aggcagctcg aaacccttgc ggtactcgcg gcccaggaac ttgttcgctt ccttgtcctc
     7981 gacggtcatc ttttcatcca tgaagcacaa ttccttctca tggttccaga aaatctgtcg
     8041 cccgaccctc agcgacacgt ttgccagatg acaggccacg acgcttgtgt aatgctggtc
     8101 gatcgacgcg atgggttttt cgcggctctg aacgcactcg aggaagttct cgacgtgggc
     8161 gctgtgtcct tccacgtcct tcttgtgata ctcgcccatc tccttgaccg gatcgatcct
     8221 cggctcggaa ccgggtcgat tccagtcctc cagatcgggt tcgacttccc accccccccg
     8281 ggtcatgtga agaaagccgt tagtgccgtg aaagtccatg ccgatgccgc ccttatgcca
     8341 cggcttcccg ctggctttgc ggagcgtgta ggtatgcacg tggcctttcg gggccagctt
     8401 cgatggtgga aacttgtaga tggtcaccat cgtgtcgggg gtgtcacggt cgtcggccag
     8461 gatgtaatgt ccgcccatcg agcagaccga cgtcggatgc ggagtgtcca gcgtccacat
     8521 gatgatgtcc tgcagatgga cgttccaatc acctaccatt ccgcagccgt agtcgaaata
     8581 ccacctccaa ctactgtgga aacgacgttt gttaaacttg cgctccgggg ccggaccaag
     8641 ccagaggttg tagtccacgg tggggggcgg atcggaatcc ggttcgttgc caagaccgtc
     8701 gggggattca tttccgtaag tgtaggtatt ggtcgcgctg atctcaccga gttcgccgtt
     8761 acggacgaat tcccgggcgg cttgaatatg ctcgtagctg cgctgatggg tgccgacctg
     8821 gacgacgcgc ttgtacttct ccgccgcggc aaccatagcc ttgccctcgc ggatgttgtg
     8881 acagcagggc ttctcgacgt agatgtcctt ctccgcctgg catgcgagca gcatcggggc
     8941 ggcatgccag tgatcggggg tcgcaatgat gacgatctcg acctctttca tgtcgagaac
     9001 cttgcggaaa tccttgaagc gttgaggctt cttctgcccg gcggcgtcct tcagcttttc
     9061 ggtgatgacc gcttcagcct tgtccatctg ctcggtgtcc acatcgcaca aggccaggac
     9121 atcaacctgc ttggtatcca ggaaattgcc gagcttatgg cggcccatgc cgccgcagcc
     9181 gatcagggcg atgccaagcc tcttgttggc gccggccgcc caagctgaag aaagcatcga
     9241 tcggggcccg agaacgctca aaccggcggc cacgccggcc accgtcttgc cgctattgcc
     9301 cagaaactgt cgtctcgtca aggggttgct cattgcgttt attctccatt cctgttccgg
     9361 tctctcgcta ccggctgttc gtcttcgcgg ccgcgggcaa gcacccgcac tgaggaccca
     9421 taagttacac ccaaatgccc atccggacaa gagatacaag ccccacgccc gcgcacggcg
     9481 gccgctgaca gttggagcgg tggcgtcaga gcccgttcca ataggcaaat ctgtggcctc
     9541 gcgatctcgc tgcacgacgc cttcggccga ctggtcgatc ggccggtgta tgagaggtac
     9601 aacagtgagt tcatgagcag cacgctcgat cacttcatcc agccggccga gggagccagg
     9661 gtcagcttcg ccgggcggta tccgcgtgac tacctgtcat ccgatccgcc gcgtcgtttg
     9721 ttggcctggc acttggtcgg ggggttggat ccgctggacg cctctgagct taccggctca
     9781 gagcccgagg acggctatcc ggttcttctg ccggactgga ttcgccgcga tggactgaaa
     9841 tgcctcaagg tcaagctccg cggcaac
//
LOCUS       DAOTPW010000219         9810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6394_length_9810_cov_5.545361, whole genome shotgun sequence.
ACCESSION   DAOTPW010000219 DAOTPW010000000
VERSION     DAOTPW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6394_length_9810_cov_5.545361"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..2528)
                     /locus_tag="P5316_17015"
     CDS             complement(180..2528)
                     /locus_tag="P5316_17015"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HRR86713.1"
                     /translation="MWAIMLSLCMLGAGQGIPERLNVLRAQLDGLRAIQVPPELKDRA
                     AGQLNELAGAIAKGAATREEIDAIYLRMDELRTWLLSNAVDRPVPGGGEFFETSNDWG
                     VRNTSLTLTIARKDLSMCAKTPGTAWRWRPADNKDIELRNGKSFSLMEARRKEAGAFH
                     TGFSTGMRIMLAAFPAAPNARIDVVVHLIGNEAVFEIVPVEDISNLATLAFPKVLETG
                     NTETDVAVIPRMQGILIPGNWRQEIRYRDLTNSRALYMPWWGQLAGERGVQTILETSD
                     DAGAEYAHVPGEPTRIQPLWHSSMGRLRYPRLVRYVFDDNGGYVRMAKRYRRHVQETG
                     QFVSLAEKRIRTPNLERVIGKPVVHIGALYHNMKGSRFYSTTGMESNHRVQTFENLAA
                     SLRALKVAGIAEAYVHLDGWGFYGYDNAHPDVLPPGPEQGGWNGMRLLADTCDSLGYL
                     LAVHDQYRDFYFSAASYDDKLALLNHDGTLTEHAEWSGGRQTLLSPRFAPGYVRRNHD
                     LFGENGVKIKGAYLDVFAVVPLEESYQPSHPVTRSDCARHRRECFDLLRARGYVVSSE
                     EPADYLVRSLDLVHHGPYPTLPKGYGQGQGQGIAVPLFNLVYHDSILLPWELTEDGGW
                     GIPSGDSGRLHCLLNAGLPYVSPNASPQTIAQVQEVLSVVGRLNTQEMTDHRFLDPAR
                     RVQETTFADGTRITVDFAQKTYTVTLPGATRPGIVSETGTIRNGDVASFRSGVSVSRR
                     ISLRSTIRDEPGILGTGYWGQSPMGDIGDSHLLLILGTVTYY"
     gene            complement(2740..4788)
                     /locus_tag="P5316_17020"
     CDS             complement(2740..4788)
                     /locus_tag="P5316_17020"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRR86714.1"
                     /translation="MNDRLRCPLPLLGAGMAVLMAGGSRLFGLDFKAESTMWLDDIET
                     AAQAGSQFPFSFTYDDRPSGDLLRVWKSRTSESLPAVAGNSSDPACLRGRATAWTDPN
                     TGLRVAWQVTRFSDFPAIEWLLTFENTGTADTPIIENIQALDVSLNQPLDDRGTYRLC
                     RTNGAPSNPTDFEVRTITLTPGSTQTMSGGGGRSSNKDFPFFKIETKAGSLIVAVGWS
                     GQWAACVSSPQAPSLHLTAGIEKTHFKLLPGEKVRMPRILMLHWEGDTRESNARFRQL
                     IYKHYAAMKAGKKPLPTLFCNTCFTRGGGWLNECNAENQISLIKAYAPLGLEALLTDA
                     GWFTGGWPNGAGNWDPRKDAYPDGMGPVAAAAKAHNMIYGLWFEPERVVAGTSLHKEH
                     PDWVLSPGGENPRTFLADFGRKEVQDYFFNIVRGFMALPGFRVYRQDFNMDPLAYWRH
                     NDAPDRQGITEIKYIEGLYAYWDRIAHTWPDSLREECASGGRRIDLETVMRMHLHQDS
                     DYWFDNDVDQAQIWGLSQYLPNNVFVGHINRMDDYSFHSVMASSLCMGWIADGPDFDK
                     ARGKKLLDRYLALRHLLIGAWYPLLPYSRSGNEWIASQYHRPDLDEGMILAFRRAESP
                     YRTTDVSLDGLKPDAVYELTSQATGQKTQAKGADLMKQYQLTIPDRHKSELITYRTAK
                     "
     gene            5267..7048
                     /locus_tag="P5316_17025"
     CDS             5267..7048
                     /locus_tag="P5316_17025"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRR86715.1"
                     /translation="MSHLNRPYVDGLSWLRAAGSLWLAAVLLSLISVAMACATVFESV
                     HGSEKALAFFYKSWWFESLLGLLAINAVAALAVRYPFSRRQFGFAVAHGSILLILAGA
                     WVSQKFGIDGRVSIVEGQSVADITVPQDVFILENQSSKFSVEISPPPLLGAGTAEVPG
                     QPAVSAESVHAQVLQFASDSVARTEMRDDNPHAGLAVEVSFSETGTDEPVWVPAGQVV
                     QAGEVQVACIEITDEQELKKHTSTAPAAEEDATRTVRVEYRGNSYELKVDDCIAATQP
                     VGDTGMTLRVVRYLPHATVAGRGQITNASAKPLNPAIEAELSGPQGIERRFAFARFPD
                     FQSMHGNLKNPDVKLVLTARVDEDVHAPVEILVGPGDRLHARFTSGRDSLVQELQAGT
                     PLQTPWPQRKLGVWRIFKNARQHRTVSPSAHLHPEPRPAILVRLDSGQESMEVWVQKY
                     SDQAVVFAGQTYRLGYDDKTIPLGFKVALDAFRIRNYPGTSRPRSYESRITITDPASG
                     RLESRVISMNHPTSYGGYTFYQSSYRQAGQHTASVLSVSRDPGKEIVFAGYGLMVLGV
                     LLVLIDRLRNTAGQASARANRDGKEAA"
     gene            7048..8853
                     /gene="ccsA"
                     /locus_tag="P5316_17030"
     CDS             7048..8853
                     /gene="ccsA"
                     /locus_tag="P5316_17030"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRR86716.1"
                     /translation="MRLLQLLFLASLAQTTAPASPTIPKSLDFSIIRAIPVQHDGRWM
                     PLDTLARDMVESVTGTARWHGHDPVALLLAWTFDSGTWMDQPLIEIKNPKLREALELP
                     GNRTVFSYNALVGHPRFRRLMDDLAMVQGRKLDPLESKVRDIRERLSCLDVVLAGQAI
                     NLIPHPNDPLGAWKPIELVAGDQAADDPTKTAWASVGGAFLRGDGPAFVEACERLRSV
                     LASLPAAYRPSPDLIAVELRFNRLHLLGLSWKIMLVGAVSGLVALVLRKRTPDVAAIA
                     ALVAGFAVLSYGLWLRWQIAGRIPASNMFESLLFMGWGTGFFGILWILFVRDRMVLLT
                     ASVVSAVSLALADCLPLDPYIRPTPPVLMDTIWMSIHVPVIMVSYAVLAVAFVIAHGQ
                     MFVMAATPERSQLVKTIDRLHYLYVLAGAFLLLIGIATGSMWAASSWGRYWGWDPKEV
                     WSLIAFLAYLTILHVRASHEKTPSWLYLFALVLGAAVFGMVAARLAPLTMMRWLGLAS
                     AAVAVVVCALARGPLGDAVKSILAFWLIIMTYVGVNFVLGIGLHSYGFGTGAVVRYLF
                     LFGAIDLILVLLCVGIYYARRRVRSGHAPSFTGQQ"
     gene            9192..>9810
                     /locus_tag="P5316_17035"
     CDS             9192..>9810
                     /locus_tag="P5316_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86717.1"
                     /translation="MSSLPTRVYVYYTPSALWCGFRCEGTPGDKLEAQFTQRDDKVWR
                     DDSVDLILNHAGDPEKSAHISVNCAAVVYDALGGDASWNPELVVQTTRDGDGWSAVIG
                     VPFRALGVAVPKDGDAWAVNFCRSTSGRQLSCWAPVLKSYVEPERYGRLVFGGRRTSA
                     AQMHELDPVNIGRNRPTIVCPAGSTFRITGYDIRYRPVLEEHGPIG"
BASE COUNT         1794 a         2964 c         3089 g         1963 t
ORIGIN      
        1 tcacactaca aaccatcaca cccggtgtca ataataggtg actgtcccca atatcccgtc
       61 ataataggtg actgtcccca atatccccaa tatcaccaat atcccgacat gctctcacac
      121 tacaaaccat cacacccggt gtcaataata ggtgactgtc cccaatatca ataccggtgt
      181 caataatagg tgactgtccc caatatcaat aataggtgac tgtccccaat atcccccata
      241 ggtgactgtc cccaatatcc cgtccccaat atccccggct catccctgat cgtcgaccga
      301 agcgagattc gccgcgacac actgacccca ctccgaaaac tagctacgtc cccgtttcgg
      361 atcgtccccg tttcggatac gatacctggg cgggtcgcgc cagggagcgt gacagtgtag
      421 gtcttctgtg cgaaatcgac ggtgatccgc gtgccgtcgg cgaaggtggt ctcctgcacg
      481 cggcgggcgg gatcgagaaa gcggtggtcg gtcatctctt gggtgttcag ccgtcctacc
      541 acggacagca cttcttggac ttgagcaatg gtctgggggc ttgcgttggg cgagacatac
      601 gggagcccgg cattgaggag gcaatgcaga cgaccggagt cgcccgacgg gatcccccag
      661 ccgccgtcct cggtgagctc ccagggcaac aggatggaat cgtgataaac gaggttgaac
      721 agcggcaccg cgatgccctg accttggcct tggccgtaac ccttgggaag cgtggggtac
      781 ggaccatgat gcaccaggtc cagagaacga acgaggtaat cggcgggctc ttcggaactg
      841 acgacgtagc ctcgggcccg caagaggtcg aagcattcgc gccggtgacg ggcgcagtca
      901 ctgcgggtaa cggggtggct gggctgatag ctttcctcga gaggaacgac cgcgaagacg
      961 tcgagataag cccccttgat cttgacgccg ttctcaccaa acagatcgtg gttccgtcgc
     1021 acatagcccg gcgcaaaccg cgggctcagc aaggtttgtc gtcctccact ccactcggcg
     1081 tgctcggtga gggtgccgtc gtggttgagc aatgccagct tgtcatcgta cgatgcggcg
     1141 ctgaagtaaa agtcgcggta ctgatcgtga acggccagca ggtaaccgag cgaatcgcag
     1201 gtgtctgcca gcagacgcat tccgttccag ccaccctgct cgggtccagg cggcagcacg
     1261 tcggggtgag cattgtcgta gccatagaag ccccagccat cgagatggac gtaggcctcg
     1321 gcaatgccgg ctaccttcag ggctcgcagc gaagccgcca gattctcgaa agtctgtacc
     1381 cggtgattgc tctccattcc agtcgtgctg tagaaacgcg agcctttcat attgtggtac
     1441 agcgcaccga tgtgaaccac cggcttgccg atcacgcgtt cgaggttcgg cgtgcgaatc
     1501 cgcttctcgg cgagcgagac gaactgcccc gtctcctgca cgtgccgccg gtatcgcttg
     1561 gccattcgca cgtagccgcc gttgtcatcg aagacatagc gaaccaagcg cggatagcgc
     1621 aaccgtccca tgctcgagtg ccagagcggc tgaatccgcg tcggctcgcc gggcacatga
     1681 gcgtactccg cgcccgcatc atcgctggtc tccaggatcg tctggacgcc cctttcgccc
     1741 gccagttgac cccaccaagg catgtacagc gcgcgagagt ttgtgaggtc gcgatagcgg
     1801 atctcctgcc gccagttgcc gggtatcaga attccctgca tgcgcggtat caccgcgacg
     1861 tctgtttctg tattgccggt ttcgagcact ttggggaacg ccagcgtcgc cagattggag
     1921 atatcttcga ccgggacgat ctcgaacacc gcctcgttac cgatcaaatg gaccaccacg
     1981 tcgatgcggg cattcggggc ggcaggaaaa gccgcgagca tgatccgcat gccggtgctg
     2041 aatccggtgt gaaacgcacc ggcttctttg cggcgcgcct ccatcagcga gaaagacttc
     2101 ccattgcgca gctcgatatc cttgttgtcc gccggacgcc accgccatgc tgttccgggg
     2161 gtcttggcgc acatggacaa gtctttcctg gcaatcgtca gggtcaagct tgtgttcctc
     2221 acgccccagt cattgctcgt ctcgaagaac tcgcccccgc cgggcaccgg gcgatcgacg
     2281 gcgttgctca gcaaccatgt gcgaagctcg tccatccgga ggtaaatcgc atcgatctct
     2341 tcacgtgtgg cggcgccctt ggcaatcgcg ccggccagct cgttcaactg cccggccgcg
     2401 cgatccttca attcgggggg cacctggatt gctcgcaaac catccaactg cgctcgcagc
     2461 acgttcaacc gttcaggaat accctgacca gccccgagca tgcagagaga aagcatgatc
     2521 gcccacatgg atgtcgacct ccttcaggag tatgcgcggt ctcctcgcac ccggagctct
     2581 ccggatgtcg tgcctaacaa ccgtcggtgc ccgatacccg atatcatcgt caaacatggc
     2641 ccgcttggcc aggaagcagc aatgataaag aggtcgagaa catagcggtc cgcagagcca
     2701 gcacctgcct cgctcagggg gccgacgagg gcgtcggccc tactttgccg tgcgatacgt
     2761 gatcagctcc gacttgtgcc tgtccggaat cgtcaactgg tactgcttca taaggtccgc
     2821 gcctttggcc tgcgttttct gcccggtcgc ctggctggtt agttcataaa cggcatccgg
     2881 tttcaggccg tcgagggaga cgtcggtcgt gcggtacgga ctctcggccc ggcggaacgc
     2941 aaggatcatg ccctcatcga ggtccggccg gtggtactgc gaggcgatcc attcgttgcc
     3001 gctccgggag tacggcagca acgggtacca cgccccgatc agcaggtgcc gcagggccag
     3061 gtacctgtcg agcagcttct tgccgcgagc tttgtcgaag tccgggccgt cagcgatcca
     3121 gcccatgcac agcgacgaag ccatcaccga atggaacgaa taatcatcca tccggttgat
     3181 gtgcccgacg aagacgttgt tgggcaggta ctggctcaag ccccagatct gggcctggtc
     3241 gacgtcgttg tcgaaccagt aatcgctgtc ctggtgaagg tgcatgcgca tgaccgtttc
     3301 gaggtcgatc cgacggccgc cgctggcgca ctcctcgcga aggctgtcgg gccaggtgtg
     3361 ggcgatgcgg tcccagtagg cgtacagccc ttcgatgtac ttgatctcgg tgatgccctg
     3421 gcggtccggc gcgtcgttgt gccgccagta ggccagcgga tccatgttga agtcctggcg
     3481 atagacgcgg aagccgggca gggccatgaa gcccctcacg atgttgaaga agtagtcctg
     3541 cacctccttg cggccgaagt cggcgaggaa ggtgcgcgga ttctcgccgc cggggctgag
     3601 cacccaatcg ggatgctcct tgtgcagcga cgtgcccgcg accacgcgtt cgggctcgaa
     3661 ccacaggccg tagatcatgt tgtgggcctt ggcggccgca gccacagggc ccatgccgtc
     3721 gggataggcg tccttgcgcg gatcccagtt gcccgcgccg ttgggccagc cgccggtgaa
     3781 ccagccggca tcggtcagca gagcctcaag cccaagcggc gcgtaggcct tgatcagcga
     3841 gatctggttc tcggcgttgc actcgttgag ccagccgccg ccgcgagtga aacaggtgtt
     3901 gcagaacagc gtcggcagcg gttttttgcc ggccttcatg gccgcatagt gcttgtagat
     3961 gagttggcgg aaccgggcgt tgctctcgcg ggtatcgcct tcccaatgga gcatcagaat
     4021 gcgaggcatc cgcacttttt cgccggggag gagcttgaaa tgtgtcttct cgatacctgc
     4081 cgtcaggtgc agcgaaggtg cctgcggact gctgacgcag gcggcccatt gcccggacca
     4141 tccgaccgca acgatcagcg agccggcctt cgtttcgatc ttaaagaacg ggaaatcctt
     4201 gttcgacgat cgcccgccgc cgccggacat ggtttgggtg gagccaggcg tcaacgtgat
     4261 cgtgcgaact tcgaaatcag tcggattcga cggggctccg tttgtccggc aaagacgata
     4321 ggtcccgcga tcatcaagcg gctgattgag gctcacgtcc agggcctgga tgttctcgat
     4381 gatcggcgta tcggccgtgc cggtgttctc gaacgtcagc agccactcga tcgcggggaa
     4441 atctgaaaag cgagtcacct gccacgcaac ccgcaggccg gtgttgggat cggtccacgc
     4501 ggtcgcccgg ccgcggaggc aagccgggtc gctgctgttc ccggccaccg ccggcagcga
     4561 ttcagaggtc cggctcttcc agacgcgcaa gagatcgccg ctcggccgat cgtcgtaggt
     4621 gaacgagaag gggaattgcg aacctgcttg ggccgccgtc tcgatgtcgt caagccacat
     4681 ggtcgactcc gccttgaaat ccaggccgaa aaggcgtgat ccacctgcca tcagcactgc
     4741 catgccggcg cccagcaaag gcagtgggca gcgcaacctg tcgttcatgg ggtacctcct
     4801 tcaacgatgc atcactgatc gccggaattc gtgtgacgaa cactcttgcc gcactagcat
     4861 atccaagggg gccaggttcg ccagcgtgtg tgatgggttg ggcgggtgcg ttcgccggct
     4921 gctccctttc tgcttcgcca aggctgcgcg ggaggggtac ggtcgcggtt gtgaaagagt
     4981 gatcagcaat cagcgcgtgg ccccagaagg gattctgggg ccagcgagat tctggggcta
     5041 gcgaaaggga ttctggggct cgcgagattc tggggctagc gaaaggcatt ctggggccgg
     5101 cgaatccaag ggggctgcgg tcgccatcct tgcctgaccg gccctatcgc tgatggcaga
     5161 tacggactat cattgggcat acggcaggcc ggatgtgaga cccgtgggaa gcaatatggc
     5221 ctccaccgga gtcggccttg gtcagaggtt gcacgagcga ttacgcatga gccatctgaa
     5281 tcgaccgtac gttgacggcc tctcctggct gcgggcagcg ggatcgcttt ggctggccgc
     5341 cgtgctgtta tcgctgatca gcgtagcgat ggcctgcgcg acggtgttcg aatcagtaca
     5401 cgggagcgag aaagcccttg cctttttcta caaatcctgg tggtttgaaa gccttctggg
     5461 gctgctggcg atcaacgcgg tcgcggctct ggccgtcagg tatcccttct ccaggcggca
     5521 attcggcttc gccgtcgctc atggctccat cctgctgatc ctggcggggg cgtgggtgag
     5581 ccagaagttc gggatcgacg gcagggtaag catcgtcgag ggccagtccg tcgccgacat
     5641 caccgtaccc caggacgtct ttatcctcga aaaccaatcc tcgaagtttt cggttgagat
     5701 cagcccccca ccgcttctgg gcgcgggtac ggcggaggtt cctgggcaac ctgcggtttc
     5761 cgcggaatcg gtgcatgccc aagtgcttca attcgcttcg gacagcgtcg cccgcaccga
     5821 gatgcgcgac gacaacccac atgccggtct agcggtcgag gtgtcgtttt cggagacggg
     5881 cacggacgaa ccggtgtggg tgccggccgg gcaggtcgtc caggcaggtg aagtccaggt
     5941 agcgtgcatc gagatcaccg acgagcaaga actgaagaag cacacatcca ccgctccggc
     6001 agccgaggag gacgcgacac gcacggtgag agttgagtac cggggcaaca gctacgagtt
     6061 gaaggtggac gattgcatcg ccgcgacgca gccggtggga gacaccggca tgacgttgcg
     6121 agttgtgcgg tacctgcctc acgcgacggt cgccggccgg ggccagatta ccaacgcgtc
     6181 agccaagccg ctgaatccgg ccatcgaagc cgagttatcg ggtccgcagg gaatcgagag
     6241 gcgattcgcg ttcgcgcgct ttcctgactt tcagtcgatg cacgggaatc tcaagaatcc
     6301 ggatgtcaag ctggttctga cagccagggt ggatgaggac gtgcacgcgc cggtcgagat
     6361 cctcgtcggc ccgggcgatc gattgcatgc gagattcacg agcgggcgcg acagcctcgt
     6421 ccaagagctg caagcgggca cgccgctcca aacgccctgg ccgcagcgca agctcggggt
     6481 atggcgcatc ttcaagaatg cccgtcagca tcggaccgta tcgccttccg cgcatctgca
     6541 tcccgagccg cgtcccgcga ttctcgtgcg actggactcc ggccaagaga gcatggaggt
     6601 ctgggtgcag aaatacagcg atcaggcggt ggtcttcgcc gggcagacct accgcctggg
     6661 atacgacgac aaaaccatcc cgctcggctt caaggttgca ctcgacgcct tcagaatccg
     6721 caactatccg ggcaccagcc ggcctcgttc gtacgaaagc cggatcacta tcaccgatcc
     6781 ggcttcgggg cgattggaga gtcgggtgat cagcatgaac catccgacga gctacggagg
     6841 gtataccttc taccaatcga gctatcgcca ggcaggccag cacacggcga gcgtgctcag
     6901 cgtctcacgg gatccgggga aggaaatcgt ctttgcgggc tacggcttga tggtattggg
     6961 tgtgctgctg gtgttgatcg accggctcag gaacacggcc ggtcaggctt ctgcgagagc
     7021 caatcgggac gggaaggagg cggcctgatg cgattgctgc aactcctttt cctggcgtcg
     7081 ctggctcaaa caacggcccc ggcctcaccg acgattccca aatcgcttga cttcagcatc
     7141 atcagggcaa ttccggtcca gcatgacggc cgctggatgc cgctggacac cctggcccgg
     7201 gacatggttg agtcggtcac cggaacagcc cgatggcatg ggcatgaccc ggtggccttg
     7261 ctgctggcgt ggacattcga ctcgggcaca tggatggacc agcccctgat cgaaatcaag
     7321 aaccccaagc ttcgggaagc gctggagctg ccgggcaacc gaaccgtctt ctcctacaac
     7381 gcgctggtcg gtcatccgcg gtttcggcgg ctcatggatg atctggccat ggtccagggg
     7441 cgcaagctcg acccgctgga atccaaggtt cgcgatatcc gggagcggtt gagctgcctg
     7501 gatgtggttt tggccggcca ggccatcaac ctgattccgc atccgaatga tccgctgggc
     7561 gcctggaagc ccatcgagct tgtcgcaggg gatcaggccg ccgatgaccc taccaagacc
     7621 gcctgggcat cggtcggcgg cgcctttttg cgtggcgacg gtcccgcctt tgttgaggct
     7681 tgcgagcgcc ttcgttcggt acttgcctcg cttcccgcgg cctaccgccc atcgcctgat
     7741 ctgatcgccg tcgaactacg ctttaaccgg cttcacctgc tgggactgtc gtggaagatc
     7801 atgcttgtcg gcgccgtgtc tggactcgtg gctctggttc ttcgtaagcg cacaccggac
     7861 gtggcagcca tcgcggcact ggtcgcgggg tttgcggtac tgagttacgg cctgtggctc
     7921 cgctggcaga ttgccgggcg gattccggcc tcgaacatgt tcgagtcgct gctgttcatg
     7981 ggctggggaa caggcttctt cggcatctta tggattctgt tcgttcgcga ccgaatggtg
     8041 ttgctgacgg cctcggtcgt cagcgcggtc tcgcttgcgc tggcggattg tctgcccctc
     8101 gacccgtaca tccgcccgac tccgccggtc ctgatggaca cgatctggat gtccatccac
     8161 gtgccggtga tcatggtgtc gtacgcagtg ctggcggttg cttttgtgat tgcccacggg
     8221 cagatgtttg tgatggccgc gaccccagaa cgttcgcaac tcgtgaagac catcgatcgt
     8281 ttgcactatc tctatgtcct ggccggcgcg tttctgctgc tcatcgggat tgccacagga
     8341 agcatgtggg cggcatcctc ttggggccgc tactgggggt gggacccgaa ggaggtttgg
     8401 tcgctgatcg cgtttctggc ctacttgacg attcttcatg ttcgggccag ccatgagaaa
     8461 acaccctcct ggttgtatct tttcgcgctg gtacttggcg cggccgtgtt cggaatggtt
     8521 gcggcaaggc ttgccccgct gaccatgatg cgatggctcg gtctggcgtc cgcggcagtt
     8581 gcggtcgtcg tttgcgcgct ggccaggggg ccgctgggcg atgcggtcaa aagcatcctg
     8641 gctttctggc tgatcattat gacgtacgtt ggcgtcaact ttgttctggg catcggtctg
     8701 cacagctatg gattcggcac cggtgcggtc gtacgttacc tgtttttgtt cggggccatc
     8761 gacctgatac tggtcctgtt gtgcgtgggc atttactacg cgaggcgccg tgtccgatca
     8821 gggcatgctc cgagtttcac cggtcagcaa tagcgaaacc aacgcaaagc gaggtctgtt
     8881 caacaatggg tcatgcgagt caatacgaac gtgatgagtt tttcgcgcac gtgacgtcca
     8941 ccgggcaagg gcgaattcgt cgaccgagac ggcgaacact gcgatctctg gcggtgatct
     9001 acgttctggc ggcaacaagc gcgacagttc ggtcagcgtc aaccgccggg cccgacgctg
     9061 ccaacgagga acgttgcctt gtccgaattc catggaccga tcgacgcgag accccgggca
     9121 cggactttgc gcagagacca tggactcgtg cggcggtcgt cagtggtttt ctgcaaatag
     9181 gcggactgag gatgtcctcg ctgccgacgc gggtgtacgt ttactacacc ccatcggcgt
     9241 tgtggtgcgg ttttcgctgt gaagggactc ccggcgacaa actcgaagca caattcaccc
     9301 agcgcgatga caaggtctgg cgcgacgaca gcgtggatct gatcctcaac cacgcgggcg
     9361 atccggagaa gtcggctcat atctccgtca actgcgccgc ggtggtgtac gacgctctcg
     9421 ggggggatgc ttcgtggaat cctgagcttg tcgtccagac aacccgggac ggcgacggtt
     9481 ggtcagccgt catcggcgta cctttcaggg ctttgggcgt ggcggttccg aaggacgggg
     9541 atgcgtgggc ggtcaacttc tgccggagca cctctgggcg gcagttgagc tgctgggcac
     9601 ccgtcttgaa gtcctacgtc gagcccgaac gctatggtcg gctggtgttc ggcggtcgcc
     9661 ggacgtcggc cgcacagatg catgaactcg atccggtgaa catcggtcgc aacaggccca
     9721 cgatcgtctg cccggcgggc agcaccttcc ggatcaccgg ctatgacatt cgatatcgcc
     9781 cggttctcga agagcatgga ccgatcgggg
//
LOCUS       DAOTPW010000220         9763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6428_length_9763_cov_5.528739, whole genome shotgun sequence.
ACCESSION   DAOTPW010000220 DAOTPW010000000
VERSION     DAOTPW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6428_length_9763_cov_5.528739"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="P5316_17040"
     CDS             <1..1628
                     /locus_tag="P5316_17040"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRR86718.1"
                     /translation="ARRERGGEGMGHRPRDTGTPFVVVLLLCGLIYSGTAKADQYHGM
                     NAGAPVWNGSIEFTQAKAAAIAATGCRAVRVNFRLDGHTSWDSGILATYDTVISTALS
                     HNLTVLGLFSNECMAVGQTSWNDDPDGDGMNAYVVNFSNTVLLLVNRYKNQIKRWEIW
                     NEPNCWTTDPNTVDPRYAGGTYILPCVYANLLTESYKQLEYYEGRSILTDNKISLVSG
                     GLFAHEIGGSFSHAMDYMQQVYARTAVWNWMEANTGRRYPWSLFGYHFYLASDTIITT
                     TRLNQYFNQVRVTQAANSDFSPIYVTEFGWNSAGGAGESGQAQNMRIAYDHMETKPYI
                     VGAYWYQWVDEPFPYYWGVTRANGSLKPSHTEFTLQNQDNIPPPEPQVSITADKLHVA
                     VGEQVSFTPTVSLFPGASALQGTWHFGADQQTVSGMPGGILRAFGQTGDYNVWLIVTD
                     SNHLEGVSDSITVHVASIPHSAADFDEDGDVDQIDFGHFQTCLTGPGANQYGPSCMNC
                     RLDDDGDVDQDDLALFRSCLSGPGVPADPQCGH"
     gene            1665..3284
                     /locus_tag="P5316_17045"
     CDS             1665..3284
                     /locus_tag="P5316_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86719.1"
                     /translation="MRTRQPVAQIGLLALVAMGAACGLHGSVFADEYHGINAGAPVWG
                     GWIYFTDARAQQIAATGCRAVRINFRMDWHQTWDSTILGIYDAIVQNALNHDLVVLGL
                     LCNECMPVGQEAWNDDPDGDGMNQYVVDFSETALLLIDRYRTQIKRWEIWNEPNAHTN
                     PEWQTDPQHAGGTYILPRVYANLLAETYKATNYREGRSLLSRYCIELCSGGLLAYDNM
                     TGMNYMEQVYQQTAVWDWMEANVGRRYPWDLFGYHFYISQARSVSTYQLRSLFEGWPW
                     SSDSVRQVQADYGDTARVLVTEFGWNTDRVSEATQRDNMRDTYEWLEGKSYVAGTYWY
                     QWQDEPGFSWGITRSDGSHKLSYDEFDVQNGLPGPPVLPPAPVVLIVSDVFEVPYAEP
                     VHFVPSVVLAPGAVAADSLWTLGADQLAVPGLPGEIEWIFPSLGDHDVCLAVTDCHGQ
                     TGVSNVITIRVTAPLYGKCDFDRDGDVDQIDFGRFQVCFSGSGIRQTAPECMAARLDD
                     DGDVDALDFAIFSTCISGEAVPADPLCGHGF"
     gene            complement(3395..4195)
                     /locus_tag="P5316_17050"
     CDS             complement(3395..4195)
                     /locus_tag="P5316_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86720.1"
                     /translation="MRTIIVAMLAFGLVTAGAAAAVTSTTTHSPIKSGNPGSTDDYIS
                     STDCLQGKIPAVLPGDMGWHPANSDPLDQLPAFTDGAGMRATGLTGLLNDWGYANPVK
                     LVAYDMTGTDCNALGVAQINIHSSNDIDGRIFSTTVIKYSMDGGANYSLLGYFQSDPS
                     GTINSQGHPNSINMKSTMVSIFDDLGAAIIPAGVTNIEFDFYSVDNTGGQMRDPFDGV
                     NPFTGIDDGLTGAFVSPLIMEIDVFPVPEPAGLLLFAAGGLLLRRRNS"
     gene            5249..6091
                     /locus_tag="P5316_17055"
     CDS             5249..6091
                     /locus_tag="P5316_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR86721.1"
                     /translation="MGRSSGFTLIEVLVVVAIIALLIAILLPSLAQARRQARQRVCAS
                     NLHQIGVAALSYDVSYRSFPHQARVGVPGCDARNSGGNVIGAWPTSVHRTIGRFIGMQ
                     SAVKANEVFYCPFVSESDRGSVDIDREQPAGSWLSNPEAYLHITYFYYGRLNTGDAAN
                     DPAKPRGTETPSDVPFKRKCYVTKNPDSRHVLMADAVSLWWGAGPPNARWRVNHGPDY
                     SAVQAAGSISPPRLVGQNVAYGDGHVDWHKVGVLPAELRRDSTQIELYRTAILRQEND
                     LHWW"
     gene            6212..7777
                     /locus_tag="P5316_17060"
     CDS             6212..7777
                     /locus_tag="P5316_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86722.1"
                     /translation="MYKHRTFALMVLGWVLSVGTAGLGAPTYIWEYGPQDVTTLPSYP
                     GIAISSTDLINGIVPLKNNDLAVGWPDNVNGYIPVDNMPGDALCKPSPINSPNTGHPG
                     FHSATPKQNGAGLTDGLLGAHVDSVLADFLLPSGVFQFDLPAPTDIGEIRVFAQNIDS
                     YPNGRVWQRYDVYISRDTNPDPKNRLFSKLLDRVVTGNIVCAGPGTGDGWNPNVNDGS
                     QVPTIGSTLTRVYDSSSPRLAGGVTSIRFVFWPVSNTQRVNWDQWLGPIGCAGIDPFD
                     VDPFDGDGFRRSFEGSIIKEIDVLPPEGPFEICDNQVDDDGDELVDCQDPGCAGEPAC
                     KSPEFCWNIDNPLDRNPIDDDGDGLANWDDPDCAPTPWPCPPEVCDNAADDDGNTLVD
                     CDDPDCWSSPVCGPETCDNGVDDDDDGLIDCADPDCEGHPACQCTHDPVFDVDDDGDV
                     DQADFAVFQTCFTGAGGNGFENLSIDCRCMDVTGVSGQPDQAIASDDYGVFEQCASGP
                     GVPASDSCDDMPL"
     gene            complement(7847..8812)
                     /locus_tag="P5316_17065"
     CDS             complement(7847..8812)
                     /locus_tag="P5316_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86723.1"
                     /translation="MCAANASTVNSGSSATLPRKEPEVGELARLKYELVRGFLWAWGK
                     CFSLRGLYLLGTGFAVCEWLVNYKRRRRFKIHLKTGFGRGTRVIDRATARRACLQHFI
                     RTRCDKLFYLIFDKLPREEILSRVRFPREADLAEAVKDGKGIYVCLSHIGSHHVLALI
                     MALKGYRVAGVRDPNEGAMRRYVQRKYEETFPEFRAVRLLYSNMYPRDVYRCLQEGYI
                     LGSTLDIGRERGAHLRLVTAEMFGKPRDFLAGPVQIALRCGAPIYQGFVVSRPGFYFD
                     IILKGPLADPRTAEDTPETLKAIVQAYADNIAEHLAEHPDHISRT"
     gene            9110..>9763
                     /locus_tag="P5316_17070"
     CDS             9110..>9763
                     /locus_tag="P5316_17070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRR86724.1"
                     /translation="MHDQHVHTWFSNDSQADPAENVRQAVSMGLAGLTFTDHFDSHPT
                     EWPLCKYDYDGIAAAVSALRKKYDDSLFVGHGIEVCYQPEQMEKIFTFLEEHRFDIVL
                     LSVHWFHGKALHERQHWTGLDPAAATRQYLEAVLEAARFALDLKRQGRRPFDVLGHLD
                     LVKRYTVRYFGTFDIRPCGDLVDEILRTCLEADLVPEVNLSTLRQSLPEPSPAEWIVG
                     "
BASE COUNT         1848 a         2769 c         3142 g         2004 t
ORIGIN      
        1 gggcccggag agagcgagga ggcgaaggaa tgggccaccg gccaagggat accggcacgc
       61 catttgttgt ggttttgctt ctttgcggcc tgatctattc gggcacggca aaggcggacc
      121 agtaccacgg gatgaacgcc ggggctccgg tgtggaacgg aagcatcgag ttcacccaag
      181 ccaaggccgc cgccatcgcc gccaccggat gccgcgccgt gcgtgtcaat ttccggcttg
      241 acgggcacac cagttgggat tcagggatcc ttgccacgta cgacacggtg atttcgaccg
      301 ccttgagcca caacctcact gttctcgggc tgttctccaa cgagtgcatg gcggtcgggc
      361 agacctcctg gaatgacgat cctgacggtg acggcatgaa cgcgtacgtc gtcaatttca
      421 gcaacacggt gctcctgctg gtgaatcgtt acaagaacca gatcaagcgg tgggagatct
      481 ggaacgagcc caactgttgg acgacggatc ccaatacggt tgatccccgt tacgcaggcg
      541 gcacgtatat actgccttgc gtgtacgcca atctgctgac cgagagctac aagcaactcg
      601 agtactacga gggacggtca attctgacgg acaacaagat ctcgctggtt tccggggggt
      661 tgttcgccca tgagatcggc ggcagctttt cgcacgcgat ggactatatg cagcaggtgt
      721 acgcccgaac cgccgtctgg aactggatgg aagctaacac ggggcggcgc tacccctgga
      781 gcctgttcgg atatcacttt tacctggcaa gcgacaccat catcacgacg actcgcctga
      841 accagtactt caatcaggtg cgggtgacac aggcggcgaa cagcgatttc agtccgatct
      901 acgtcacgga gttcggctgg aactccgccg gcggggcggg tgaaagcggt caggcccaga
      961 acatgcggat tgcctacgac catatggaga cgaagccgta catcgtcggc gcctactggt
     1021 accagtgggt ggacgagccc ttcccgtatt actggggcgt gacgcgcgcc aacggttcgc
     1081 tcaagccctc acatacggag ttcaccttgc agaaccagga caacatcccg cctcccgagc
     1141 ctcaagtgtc gatcaccgcc gacaagttgc atgtggcggt cggggagcag gtgtccttca
     1201 cgcccacggt gtcgttgttt cccggagcat ccgcattgca gggcacctgg cacttcgggg
     1261 ccgaccagca gaccgtatcc ggcatgccgg gcggcatctt gcgggccttc gggcagacgg
     1321 gcgactataa cgtgtggctg atcgtgaccg acagcaacca tcttgagggc gtatccgatt
     1381 cgattaccgt tcacgtggca tcgattccgc acagcgccgc ggatttcgac gaggatggcg
     1441 acgtggatca gatcgacttc gggcacttcc agacctgcct gacggggccc ggtgccaacc
     1501 agtacgggcc gtcctgcatg aactgccggc tggatgacga cggcgacgtc gatcaggatg
     1561 atctcgcgtt gttccggagt tgcctgtccg ggccgggcgt gccggcggat ccgcagtgcg
     1621 ggcactgagg cgaggcgggc agaaatcggt cgcagaggcg gtcaatgagg accaggcagc
     1681 cagtagcgca gatcggcttg ctcgcccttg ttgccatggg ggcggcgtgt ggcctgcacg
     1741 ggtccgtttt cgccgacgaa taccatggga tcaacgccgg tgcgcccgtt tggggaggct
     1801 ggatctactt caccgacgcg cgggctcagc agatcgccgc cacgggctgc cgagccgtcc
     1861 gcatcaattt ccgcatggac tggcaccaga cgtgggactc taccatcctc ggcatctacg
     1921 acgcgattgt tcagaacgct ctgaaccacg acctggtggt gctggggctg ctgtgcaacg
     1981 agtgcatgcc ggtcgggcag gaggcgtgga acgatgatcc cgacggcgac ggcatgaatc
     2041 agtacgtagt cgacttcagc gagacggctc ttttgctgat cgaccgctac aggactcaga
     2101 tcaagcgttg ggaaatctgg aatgagccga acgcccatac caatcccgag tggcagaccg
     2161 atcctcagca tgccggcggc acttacattt tgccgcgggt ttatgcgaac ctgctcgccg
     2221 agacatacaa ggcgaccaat tatcgcgagg ggcgctcgct cctgagccgt tactgcatcg
     2281 agctgtgctc aggggggctt ctggcttacg acaatatgac cggcatgaac tacatggagc
     2341 aggtttatca gcagaccgcc gtctgggact ggatggaagc caacgttgga cgccggtatc
     2401 cctgggacct gttcggctat catttctaca tcagccaggc gcggtcggtc agcacctacc
     2461 agttacggtc attgtttgag ggatggccat ggagcagcga cagcgttcgt caggtgcaag
     2521 cggactacgg cgataccgcc cgcgtgctgg tcaccgagtt cggctggaac accgataggg
     2581 tgagcgaggc gacgcaacgt gacaacatgc gcgatacgta cgagtggctg gaaggcaaat
     2641 cctacgtcgc aggcacgtat tggtatcagt ggcaggatga gccgggtttt tcctggggga
     2701 tcacccgcag cgacggcagt cacaagcttt cctatgacga attcgacgtc cagaacggtt
     2761 tacccggccc gcccgtgttg ccgccggccc ctgtggtctt gattgtctcg gacgttttcg
     2821 aggttccata tgctgaaccc gtgcacttcg tgccgtcggt tgtgcttgcg ccgggggccg
     2881 tggcggcaga ttcgttatgg accctcggcg ctgatcaact cgcggtaccg gggctgccgg
     2941 gcgagatcga gtggatcttc ccatcgctcg gcgaccatga cgtttgtctg gccgtgaccg
     3001 attgtcacgg gcagaccggt gtctccaacg tgatcaccat tcgggttacg gcgcccctgt
     3061 acggcaaatg tgacttcgac cgcgacgggg acgtggacca gattgatttc gggcgttttc
     3121 aggtttgctt cagcggctcg ggcatccgtc agaccgcccc cgagtgtatg gcggcccgcc
     3181 tggatgacga tggcgacgtg gacgcgctgg actttgccat cttcagcacc tgcatttcag
     3241 gggaggcggt gccggcagat ccgctgtgcg gccatggatt ctgatcgggt gtcgccgcgc
     3301 acgaaacagc ccctgccggc gggcgtgccg gcaagggctg atctcatagc gagacgcaat
     3361 tgaggacgcc gggcgcgaac cctacgtcgc actactaaga gttgcggcgg cggagcaata
     3421 agccgcctgc cgcgaacagg agcaggccgg ccggttcggg cactgggaac acgtcgatct
     3481 ccatgatcag gggcgagacg aacgctccgg tcagaccgtc gtcgattccg gtgaacggat
     3541 tcacgccgtc gaacgggtcg cgcatctgcc caccggtgtt gtccacagag tagaagtcga
     3601 actcgatatt ggtcacaccg gcggggatga ttgccgcgcc gaggtcatcg aagatactga
     3661 ccatcgttga cttcatgttg atcgaattcg gatgtccttg gctgttgatc gtgccggaag
     3721 gatccgactg gaagtagccg agcaggctgt agttggcgcc cccatccatc gagtacttga
     3781 taaccgtggt cgagaagata cggccgtcaa tgtcgttgct gctatgaatg ttgatctggg
     3841 ctacgcccag tgcgttgcag tctgtgcccg tcatatcata cgcgaccaac ttgacggggt
     3901 tggcgtagcc ccagtcgttc agcaggccgg tcaggccggt ggctcgcata cccgcaccgt
     3961 cggtgaacgc cggcagctgg tcgagcggat cactgtttgc cgggtgccag cccatgtcgc
     4021 ccggcagcac ggcagggatc ttgccctgga ggcagtcggt cgaactaatg tagtcatccg
     4081 tgctgcccgg gttaccactc ttgatcgggc tgtgggttgt ggtcgacgtc accgccgcgg
     4141 cggcgccggc cgtgacaagc ccaaaagcca acatcgcaac tatgatcgtt ctcatgtcca
     4201 tcctcccttt cttgaaataa aacctcttct taggtttgag ttgtccccgt tttccttctc
     4261 ggcttccgca gtgggtgagc caaacgccgt caatttactg cttgtacttc agtcgctatc
     4321 gactgatcgg gagttgagag atcctgccat gcgaagcctt gtgctgaaga cgctgttggc
     4381 agagccacgg cttccttcca gatcctcttc caggttgaca tgagcttctc aaccggctcc
     4441 catttgctcc atattgtgcc ccggcagggc gggtctgtca agggatttcc ggggacattt
     4501 tgtccagggg ggcggaggcg ccgggagctt ctcctggaat cccgagtgct tggcaccgtg
     4561 aatgatgggc ctgcccgcac cgggatcgta cctcacgggg gtgaattaca tgcggtcgcg
     4621 cttctgagcg ggccggctaa agagccattt cctctcaccg tttggtgtct catgaacagc
     4681 ggcccaaccc atgccaatcg gtcgtgggag gcttcggcac cccggcacgc aggagcatcg
     4741 gtgtagcagg tgtcatggac ctgctttggg ccacgttcgc cgggcagcga acagccgctt
     4801 gttctgcggc gcgtatcccg gttgcagaca gagtcgggaa gggccagagc ttcggcgtcc
     4861 tactttgagg ccgaaggttg ggccgaggaa ggagaggccg tacatgcgtg aatggatata
     4921 atacagtaat gacggaatgc tgctgccggc atcatcgttg cttgtgccga gccgccttca
     4981 ggaaaccgca ttgatgttgc tgaagtgtct ttacaccaag acgagtaagg cataagatta
     5041 tggcttggca aagctgcgga gaagcggcga tccggcctga cggggcggat gccgtggaga
     5101 gtcgtgaggt tttgttactt gcccggaaaa agatgcggct tcaggctcgc gtgcttggtt
     5161 ttttgccaag gggacagata gcacgtgggc cggcatgagg gaatgaggga tgactcacaa
     5221 gcctgcaggg atgacaaggc gaggttgctt gggtcgatct tcgggtttca cattgattga
     5281 agtgctcgtc gttgtagcga tcattgccct tttgattgcg atccttttgc cgagtctggc
     5341 ccaagcccgg cggcaggcgc gtcagcgggt gtgcgcgagc aatctgcacc agatcggtgt
     5401 tgccgctctt tcatacgacg tgtcgtacag gagttttcca caccaggcga gagtgggcgt
     5461 gccgggctgt gatgcgcgca attcgggcgg caacgtgatc ggggcctggc ccacaagtgt
     5521 tcaccgcacc atcggccgat tcatcggcat gcagtcggcc gtcaaggcga atgaggtgtt
     5581 ttactgtccg tttgtcagcg agtccgaccg cggcagtgtc gacatcgacc gcgagcagcc
     5641 ggcgggcagt tggctcagca atccggaggc ttacctgcac atcacctact tttactacgg
     5701 aaggctgaac accggtgacg ccgccaatga tcccgcgaag ccccgcggca ctgagacacc
     5761 ttccgacgtg cctttcaagc gcaagtgcta cgtgaccaag aacccggatt ccaggcacgt
     5821 gctgatggcc gatgcggtca gtctgtggtg gggagcaggc ccgcccaatg cgcgatggcg
     5881 cgtcaaccat gggccggatt actccgccgt tcaggctgcc ggttccatat cgccgccgcg
     5941 gctggtcggg cagaacgttg cctatgggga cggacacgtt gactggcata aggtcggggt
     6001 gttgccggcg gaattgcgcc gcgactcaac ccagatagag ctctacagga cggccatctt
     6061 gagacaggag aacgatctgc attggtggtg agcggggtgt gagcttgctg gaggttctga
     6121 tcttcggcag gtgcaatatc gaacctttgt ccgagcgagc ggagaatcgg tcaaagacgc
     6181 agacaaatcg acatggaaag gagccaaagc aatgtacaag cacagaacat tcgccctgat
     6241 ggtgctcggc tgggtcttga gcgtcggcac tgcaggtctt ggcgcgccga cctacatttg
     6301 ggaatacggg ccgcaggacg tgacgacgct gccgtcgtat ccgggaatcg cgatctcgtc
     6361 cacggacctg attaacggca tcgttccgct gaaaaacaac gacttggcgg tgggttggcc
     6421 ggacaacgtg aatggataca tcccggttga caacatgccc ggtgatgctc tctgtaagcc
     6481 atcaccgatc aacagcccca acacggggca tccgggtttc cactcggcga cgccgaagca
     6541 gaacggtgcg gggctcaccg atgggttgct tggcgcccat gttgattccg ttctggccga
     6601 tttcctcctg cccagcggcg tctttcagtt cgacttgccc gcgccgaccg acatcgggga
     6661 aatccgggtt ttcgcgcaga acatcgattc ttatcccaac ggccgtgtct ggcagagata
     6721 cgatgtctac atttcccgtg acacgaaccc cgacccgaaa aaccgcctgt tcagcaagct
     6781 gctcgatcgc gtggttacgg gcaacatcgt gtgtgccggg ccgggcactg gtgacggctg
     6841 gaaccccaac gttaacgacg gcagtcaggt gccgaccatc ggttccacct tgacccgcgt
     6901 ttacgacagc agcagtccgc ggcttgccgg cggtgttacg agcatcaggt ttgtcttctg
     6961 gccggtctcg aatacgcaaa gggtgaactg ggatcagtgg ctgggcccga tagggtgcgc
     7021 cggcatcgat ccgttcgacg ttgacccgtt tgacggcgat ggtttccggc gatcgttcga
     7081 ggggtcgatc atcaaggaga ttgacgttct gcctccggag gggcctttcg agatttgcga
     7141 caatcaggtt gacgatgatg gagatgagct ggtcgattgt caggatccgg gttgtgccgg
     7201 agaaccggca tgcaaatccc ccgaattctg ctggaacatc gataatccgc tggaccgcaa
     7261 tcccatcgac gatgacggtg acggcctggc caactgggac gatccggact gcgccccgac
     7321 gccgtggccg tgcccgcccg aggtctgcga caatgccgcc gatgatgacg ggaacactct
     7381 cgtcgactgt gatgatcccg attgctggag cagcccggtc tgtggccctg aaacctgtga
     7441 caacggtgtg gatgatgacg acgacgggtt gatcgattgc gcggatccgg attgtgaggg
     7501 acacccggcc tgccagtgta cacacgatcc ggttttcgac gtggacgacg acggcgacgt
     7561 cgatcaggcg gacttcgcgg tctttcagac ctgcttcacg ggggccggcg gcaacggttt
     7621 cgagaatctg tcgatcgatt gccgttgcat ggatgtgacg ggcgtgtcgg gtcagcctga
     7681 ccaggccatt gccagcgatg attacggcgt cttcgaacaa tgcgccagcg gcccgggggt
     7741 gccggccagt gattcttgtg acgacatgcc gctctgatcg gcggtagggt ttcgcggcgg
     7801 tctgctttgt tcagcgcggc gtgacaggcc ggtgacggcg gatctgtcag gttcgactga
     7861 tgtgatcagg gtgctcggcc aggtgctcgg cgatgttgtc ggcgtatgcc tggacgattg
     7921 ccttgagcgt ctcgggtgtg tcttcagcgg tgcgggggtc ggccagcggc cccttcagga
     7981 tgatgtcgaa gtagaagccg ggccgggaca cgacgaatcc ctggtaaatt ggggctccgc
     8041 agcgcagggc gatctggacg ggaccggcaa ggaaatctct tggtttgccg aacatctcgg
     8101 cggtcacgag tcgcagatgc gcgccgcgtt cgcggcctat gtccagggtg ctgcccagga
     8161 tgtagccttc ctgcaaacaa cgatagacgt ctctggggta catgtttgaa tagagcagcc
     8221 tgacggcccg aaactcgggg aaagtctcct catacttgcg ctggacatat cgccgcatcg
     8281 caccttcgtt gggatcgcgc acccccgcca cgcgatatcc cttcagggcc atgatcaggg
     8341 ccaatacgtg gtgtgagccg atgtgactga gacacacgta aatgccctta ccatccttga
     8401 ccgcctcggc caggtcggcc tcacggggga accggacacg cgagagaatc tcctccctgg
     8461 gcagcttgtc gaagatcagg tagaagagct tgtcgcagcg ggttcggatg aagtgctgaa
     8521 ggcacgctcg gcgggcggtt gcgcggtcaa tgaccctggt tccgcgtccg aatcccgttt
     8581 tgaggtgtat cttgaaccgt cgccggcgct tgtagttgac gagccattcg caaacggcaa
     8641 acccggttcc gagcaggtac aggccccgga gcgaaaaaca cttgccccag gcccacagga
     8701 accctcggac cagctcgtat ttcagccgag cgagctcgcc gacctcgggt tctttccgcg
     8761 ggagcgttgc ggacgacccg ctattgacgg tgcttgcgtt tgctgcgcac atagttcaac
     8821 cggcgacagt atactgcgaa gacgcgtttc cgcaacgatc gagaaccgag cggctcgtcc
     8881 gcgggccttt atccggatcg caagcgccta tagactgtgg cttcgctgtg gtcggctgac
     8941 gacggtgaga ctggaaggaa gccgccggat gcgggttttg ctatggcggt aaggccgcat
     9001 cagtctcttc cgtcgggact gggtgcgcat tttgcgcgca tgtttctgga tcgcagaacg
     9061 tttgggttgc ggcccgcaaa aggcggtccg ccttaggtga gaggtggttt tgcacgatca
     9121 gcatgtacac acgtggtttt cgaacgactc gcaggccgat ccggcggaga atgtccgaca
     9181 ggccgtcagc atgggattgg cgggcttgac cttcacggat catttcgaca gccatcccac
     9241 ggagtggccg ttatgcaaat acgactacga tgggattgct gcagcggtct cggccctgcg
     9301 gaagaagtat gacgacagcc tctttgtggg gcacggcatc gaggtctgtt accagccgga
     9361 gcagatggaa aagatcttca cgttcttgga ggagcatcga ttcgacatcg tgcttctgag
     9421 cgtccactgg tttcacggca aggctctgca tgagcggcag cactggacag ggttggatcc
     9481 ggcagcggcg acgcggcaat acctggaggc ggttctggag gcggcccgtt ttgcgctcga
     9541 tctgaagcgg caagggcgca ggccttttga cgttctgggg catcttgatc tggtcaaacg
     9601 gtatacagtt cggtatttcg gtacgtttga catccgcccg tgtggggacc tggttgatga
     9661 gatcctgcgg acctgcctgg aggcggatct ggtgccggag gtcaatctaa gcacgctgcg
     9721 tcagtcgctt ccggagccgt caccggcgga atggatcgtc ggt
//
LOCUS       DAOTPW010000221         9757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6432_length_9757_cov_5.453412, whole genome shotgun sequence.
ACCESSION   DAOTPW010000221 DAOTPW010000000
VERSION     DAOTPW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6432_length_9757_cov_5.453412"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(604..1989)
                     /locus_tag="P5316_17075"
     CDS             complement(604..1989)
                     /locus_tag="P5316_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR86725.1"
                     /translation="MSGRKERILIVDDDKIIVESLAEFLDLEGYEAAGAANFAEAVTA
                     LERKHYQLVITDVNMPDTDGFELLRLIKQRYPDIVVIMITGYGTIESAVEAIKMGAYD
                     YLTKPIIDDEIRLVVERALQQQSLIRENRSLREALDLRYNLDNIVGHDYKMLKIFDLI
                     EAVAESRTTVLIQGESGTGKTMIARAIHHRSDRRDKPFVEVSCGAIPESLLESELFGH
                     VKGAFTGALGDKQGKFKAADKGTMFLDEISSATPALQVKLLRVLQERQFEPVGSNKTE
                     NVDVRVILASNDNLSQAVAAGRFRQDLYYRVNVVTIQLPALRERIGDIPLLAEHFLKR
                     YCSEGRKQILGFTEECIQAMQRYNWPGNVRELENVVERAVVLTRNRRIEVDDLPPNVI
                     EAAEAAPAVEGGYRPMSLKEALEEPEKRIIEQALRANNWNRQITAQVLEINRTTLYKK
                     MKRYGLESESM"
     gene            complement(1986..3170)
                     /locus_tag="P5316_17080"
     CDS             complement(1986..3170)
                     /locus_tag="P5316_17080"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR86726.1"
                     /translation="MAGTHPDGTDEKATLPDPKYDLGFTGERVLTCLSLGLVVFDRNL
                     NIIHHNPAAEYLVSGCRSIAEALARGTVDARYQDWRQALMGVIERGREQRFDQVVRQD
                     NQQGERLLNLLCIPWARESGGEVSGGILVIEDVTAVVGMEKRLAVSERMAAVGNLAAR
                     VAHELNNPLDGILRYINLALRAGEIGRPEKITEYLTQARGGLLRMTEIVRELVEFSRS
                     TAAAFDDAGINTIVDEAVKVMSDKAVAAGVSIVCTLSEDVPAVRGTNLFQVFCNLIKN
                     AIDAMPNGGTLTIATQVVDGEAIIRFEDNGIGLPEEMDRIFEPFFTTKPPGKGTGLGL
                     PICKDIVEKYNGKIIPQRRPQGGTAFTIRLPLQSCTAMRPSAGSGAVRSRFSRAPREA
                     KT"
     gene            complement(3359..4012)
                     /locus_tag="P5316_17085"
     CDS             complement(3359..4012)
                     /locus_tag="P5316_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86727.1"
                     /translation="MEAVATIAIPGNCHNSCQPRLAGRLIRIAALMLVCACPAAVGCS
                     GLKSGARVVGEVPAWQLAGAQPSTIRPLGRCRVDAERQVPANLVTPLCEEFSLVLVSH
                     ATDWLLLCSRCQLTGTPPSLDFENGAVVGLIANVGESASQDWPIHLQTARVFDGEGLL
                     EFSFSGGIYHPVRTAGYFELAYVAGLRSVQRVRVGRRDFILSPRGIEDQLDSKWSSE"
     gene            4362..5750
                     /locus_tag="P5316_17090"
     CDS             4362..5750
                     /locus_tag="P5316_17090"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRR86728.1"
                     /translation="MRKLIGPLVLAVLLVIAYFMVKNFVRVPISAIKPEFDVVRRGDL
                     IVPITASGNIRPASVTNIKSEASGEVIELPFDLDQYVRKGDLVVRLDETDEQRNVARA
                     TAEYQRAVIAYEQAKIRKDLNEKVGVDLAKAKLEQAEAQEDLAKLENDRQERLKATDI
                     TGQYEAAMVRAKYKQAQAATLAAKAQLSEAQLNVKMAEKEVAAADEMQKAAQRAKEEA
                     EERLKETKVLSPLDGVVVARHVQIGELVQSGRTSLTGGTVLLEIADVSEVYAVVNVDE
                     ADIGQVRQLFTETNPRVNPAATQLATLPEGTIETGQKVDLTVETYPADEGFYGVIERI
                     SPQSEMSRAIATFKVWIRVTSPNRDKLKKVLNAQVQANFTARSVANALLVNYEAMKPD
                     PSGQGYGVYVPEKVPGTNDEKPKFVPCEFGVDNRVDVEVKKGLEEGQRVYTKLPIKTE
                     REKKRERQQSEE"
     gene            5785..6546
                     /locus_tag="P5316_17095"
     CDS             5785..6546
                     /locus_tag="P5316_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR86729.1"
                     /translation="MIRITNLTKFYQMGDSVVHALDGVDLSVEEGEFVSITGASGSGK
                     STMMHLIGCLDRPTTGEYVLAGQRVEKLSDRRLAGIRNKYIGFVFQTFNLINRTTAMD
                     NVALPLVYARQGHARRLASAALERVGLGHRGGHKPSELSGGERQRVAIARALVNKPPL
                     LLADEPTGNLDSKTGEQIMSLFHELHQQGVTIVLVTHEMAIAAQAQRVIRMKDGKIIE
                     DRRVDEAFREQLLAERIAATTARLTEQSRRPPAAR"
     gene            6562..7785
                     /locus_tag="P5316_17100"
     CDS             6562..7785
                     /locus_tag="P5316_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR86730.1"
                     /translation="MFALQIMIMALRGLQANLLRSLLATLGVIIGVGAVISAMSILEG
                     AQRDMIERFESLGSETITVIPNVAKSGGRAVGMAETLVLEDADWIADPRNCPSIKAVA
                     PEVSGFDVIKYFSRNSEVSILGTNEDYAPMFNYRVAQGRFLTRDDVASHQKVAVLGYQ
                     VALDLFGRAEAVGHTIRLKGASFRVIGVMEKKGNIGFRTVDSQVIIPITTAQSRLFGI
                     KFINSISAQAVETKLVDQAIDEIKGELRRRHDIRFRSGQEDDFRVFSQEEQRRQIGEI
                     VFLFQVVLYSIALISLVVGGIGIMNIMLVSVTERTREIGVRMAVGAQRSDILLQFLIE
                     AGTVSLLGGAFGVGCGFAFNNLLEETTQIIETYTPARALVWALSMAIGIGILSGIYPA
                     YKASRLDPVEALRYE"
     gene            complement(8109..8903)
                     /gene="dapB"
                     /locus_tag="P5316_17105"
     CDS             complement(8109..8903)
                     /gene="dapB"
                     /locus_tag="P5316_17105"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRR86731.1"
                     /translation="MIGVAITGAAGKMGLRVVALAQDLKDVEVEAALEMTGHPAIGRD
                     IGELAGLGKTGLPLQDRTDTEFDVLIDFSRPAGTMHWLDYCLVKKRAIVIGTTGHTPE
                     QNNAIEEAAKTIPVLKASNMSVGMNLLFKLVGQVASILGDDYDIEIVESHHRFKADAP
                     SGTALSLRDSIVKATGRDADKDVIYGRKGDTGRRPPRQIGMHALRVGDTVGEHEVHFG
                     NLGETVRISHSAHTRDTFARGALRAAVWLAGKPAGRYDMMDVLGLK"
     gene            9114..9746
                     /locus_tag="P5316_17110"
     CDS             9114..9746
                     /locus_tag="P5316_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86732.1"
                     /translation="MARLRRNRIMLKWHPSAMRRALTVSALIMSALVVVVRIRLIPQF
                     WRYTDGIYFDTLLWFVCIPPIILLWLDLRRVFPPGHCQECGYDLAGKTSGVCPECGET
                     ISERPAPEKRHLSRARLGRTGRMLGRKGRVLKWTGAATCTVIFLAWAASVPLQFGYVY
                     AYGAPGSRQGSYHIHFNDGRINISHLNHRSPYGSYGYCQRRSESAEISPV"
BASE COUNT         1930 a         2904 c         3017 g         1906 t
ORIGIN      
        1 ggggccggtg gatttgcggg cgttggcgcg attggcggcg attctggcgg gggaggttgg
       61 gttggcggat ttctttgtgg acattggcgg ctccttcgtt tcacaagttg tcagatatta
      121 gctgtcagcc attccttcgg tgggcggagt tcgcccactg cgagatgaac acgcggggtg
      181 ttccggacag gcggatcgga gcggagcaga cgtcccaggg gcttgcgccc ctggcacctg
      241 tgcccctttg ggcagaaggc ccgttgatag ccggggaagg cgagaaaggt gcatggtggg
      301 aggcgagttg gtccccaaac gaatcgttag tctcttccgt cgggaccggt gcgcgttctg
      361 ggcgcatgtt ttggttgcgg ccgaaggccg ggttgggctg ttagtctgtt gggttgttgg
      421 tctgttggtc tgttggtctg gtaggtcggt agaagaagac ggaggctttg accagtggcg
      481 agagactcgt ccggaccggt agggtgttgg ggtgttgggg tgttgggcct ggggcggccg
      541 gggcctgccc taccgggctc atcggtgagt gccgactact gatcgctacg atccggcatc
      601 ttctcacatc gactcggatt ccaggccgta gcgtttcatt ttcttgtaga gggtcgttcg
      661 attgatctcc agcacctggg cggtaatctg ccggttccag ttgttggctc gcagggcctg
      721 ctcaatgatt cgcttctccg gttcttccag agcctccttc agagacatcg gccggtagcc
      781 gccctcgaca gcgggcgcag cttcggcagc ctcgatcacg ttcggcggca gatcgtcgac
      841 ctcgatccgc cggttgcgag tcagtaccac ggcccgctcc acgacgtttt cgagctcccg
      901 aacgtttccc ggccagttgt agcgttgcat cgcctgaatg cactcctccg tgaagcccag
      961 gatctgcttg cggccttctg agcagtaccg tttcaggaaa tgctccgcca gaagcggaat
     1021 gtcaccgatc cgttctcgga gggccggcag ttgaatcgtg accacgttga cgcgatagta
     1081 aaggtcctgc ctgaaccgcc ccgccgcaac ggcctgcgac agattgtcgt tgcttgccag
     1141 gatgacgcga acatcgacgt tctcggtctt gttcgagccc accggctcaa actgacgctc
     1201 ctgcaaaacg cgaagcaact tgacctgcag agccggcgtg gccgaggaga tctcgtccag
     1261 gaacatggtg cccttatcgg cggccttgaa cttgccctgc ttgtcaccga gggcaccggt
     1321 gaaagctccc tttacatgac cgaaaagctc gctttccagc agcgactcgg gaattgcccc
     1381 gcaactgacc tcgacgaacg gtttatcgcg gcggtcgctc cggtgatgaa tggcccgggc
     1441 gatcatcgtc ttgccggtac cggactcacc ctggatcaaa accgtggtcc gcgactccgc
     1501 caccgcctcg atcaggtcga agatcttcaa catcttgtag tcgtgcccga cgatgttgtc
     1561 gaggttgtaa cggagatcca gggcctcgcg caggctgcgg ttctctcgaa tcagtgattg
     1621 ctgctgaaga gctcgctcga ccacgagacg aatctcatcg tcgatgatgg gcttggtcag
     1681 gtagtcgtag gcccccatct tgatcgcttc gaccgcgctt tcgatggtgc cataaccggt
     1741 gatcatgatc acaacgatgt ccggatacct ctgcttgatc agccgcaaca gctcgaagcc
     1801 gtccgtgtcg ggcatattca cgtcggtaat gaccaactga tagtgtttcc gttccagagc
     1861 ggtgacggcc tcggcaaagt ttgccgcgcc ggccgcctcg taaccttcaa gatcgaggaa
     1921 ctcggccaga gactccacga tgatcttgtc gtcatcgaca atcagaatcc gttcttttcg
     1981 gccactcacg tcttcgcctc cctaggggct cttgagaacc tagacctgac cgccccggag
     2041 ccggcactcg gacgcatcgc ggtgcagctt tgcagaggta gtcgaatggt gaacgccgtt
     2101 ccgccctggg gcctgcgctg cggaatgatc ttaccgttat acttctccac aatatccttg
     2161 cagatgggca atccgagacc cgttcccttg cccggaggct tggtagtgaa aaacggctca
     2221 aagatcctat ccatctcctc cggcagccca attccgttgt cctcaaagcg aatgattgcc
     2281 tccccgtcta caacctgcgt ggcgatcgtg agcgtaccac cgttcggcat ggcgtcgatc
     2341 gcgttcttga tcaggttgca gaacacctgg aaaaggttgg taccgcgaac ggccggcacg
     2401 tcttcgctga gggtgcagac gatggagacg cccgcggcaa ccgccttatc ggacatcact
     2461 tttaccgcct cgtcgacgat ggtgttgatc cccgcatcgt caaaagcggc ggccgtactc
     2521 cgcgagaact cgacgagttc gcgcacgatc tcggtcatcc gcagcagccc gccgcgggcc
     2581 tgagtcagat actccgtgat cttctccggt cggccgatct caccggctcg caaggccagg
     2641 ttgatgtatc gaagaatgcc gtccagagga ttgttcaact catgggccac tctagcggca
     2701 agattcccca ccgccgccat ccgctccgag accgccagcc gcttctccat gcccaccacg
     2761 gcggtgacgt cctcaataac cagaatgccc ccgctgactt cccctccgct ttcgcgtgcc
     2821 caaggaatgc aaagcagatt cagcaaccgc tcgccctgct ggttgtcctg ccggacaacc
     2881 tggtcgaatc gttgttcccg cccccgctca atgacgccca tcagtgcttg tcgccagtcc
     2941 tggtaccgcg cgtcgacggt tcctctcgca agggcctctg cgatcgagcg gcaacccgag
     3001 accagatact ctgcggcggg attgtggtgg atgatgttga ggttgcggtc gaagaccact
     3061 aagcccagcg agaggcaggt caggacccgc tctccggtga agcccaagtc gtacttcggg
     3121 tccgggagag tcgctttttc gtcggttcca tccggatgtg ttccggccat aaggtccgcc
     3181 ccgtgggaag tgatgcctgt gggcatcatc ggacgcaaag cattgccgca gagcaaccta
     3241 tacgattgtc ggagactaaa gtcaatgtga tgcgtacatt cggatcaaca ctacgggaga
     3301 aagaaggatg acttgggaca acgcgaggtg taacttgtta gcggagcgga agttatggtt
     3361 attcagagga ccatttagaa tctagttgat cctcgatccc ccggggagaa aggatgaagt
     3421 cccgccggcc gacccgcacc ctctgaacgg accttaggcc cgcaacgtaa gcaagctcaa
     3481 agtagccggc cgtccggacg ggatggtaga tccccccgct aaaggagaat tcgagcaggc
     3541 cctcgccgtc gaacacccgg gccgtctgaa gatgaatcgg ccagtcctgc gaagcagact
     3601 cgcccacatt ggcgatcaga ccgaccacgg cgccgttttc gaagtcgagg gaaggtggcg
     3661 tcccggtcag ttggcatcgc gagcaaagca acaaccagtc ggtcgcatgg ctcaccagca
     3721 ccagcgagaa ctcttcacac agtggggtaa ccagattggc gggaacctga cgttccgcat
     3781 ccacacggca tcggccgaga ggacgaatgg tgctcggctg ggcccccgcc agttgccatg
     3841 ccggcacctc gccgaccact cgggcaccag acttcaagcc gctgcagccg actgccgcgg
     3901 gacacgcgca caccagcatc aatgcggcga ttcggatcag ccgccccgcc aaccgcggtt
     3961 ggcaactgtt gtggcaattg cccggtattg ctatagtagc aaccgcttcc atggatatga
     4021 gcattgttgc ccgacaagcc gtccgattca accggccgcc aagctgcaga gtctggttct
     4081 cgatgctccg gaagatgaaa agcgggacga gaatcgttca gccatcggcg cggtcggtgt
     4141 tgatgggtct tcggggactg gcggagggca atgatcgaac gtgaggccgg cttcggcgcg
     4201 gggaccgaga agagagcctc tatcatacgg ggcgagcaga tcactctgac gccaagacgg
     4261 aaaagggcaa tacaagcgtc gcgagttgtc gatgacgcgt ctcgcgattt ggccccaccg
     4321 gaaaccgcgg gagctcccgc ctcacccgga gagactcgga aatgagaaaa ctgataggtc
     4381 ctctggttct ggccgtactg ctggtcatcg cctacttcat ggtcaagaat tttgtgcggg
     4441 ttccgatatc cgcgatcaag cccgagtttg acgttgtccg gcggggagac ctgatcgttc
     4501 cgatcaccgc cagcggcaac atccgcccgg cgagcgtcac caacatcaag agcgaggcca
     4561 gcggggaggt tatcgaattg ccgttcgatc tcgatcagta cgtgcggaag ggagacttgg
     4621 tggttcggct tgacgaaacc gacgagcaaa gaaacgtcgc ccgcgcaact gccgagtatc
     4681 agcgtgcggt catcgcctat gaacaggcaa agatacgcaa ggacctgaac gagaaggtgg
     4741 gggtggatct ggcgaaggcc aagctcgagc aggcggaggc ccaggaggac ttggcgaagc
     4801 tcgagaacga ccgtcaggaa cgactgaagg ccacggacat caccggccaa tacgaggccg
     4861 cgatggtcag ggccaagtac aagcaagcgc aggcagccac gctggcggcg aaggcacaac
     4921 taagcgaggc ccaactcaac gtgaagatgg ctgagaagga ggtggcagca gccgacgaga
     4981 tgcagaaagc ggcgcaacgt gccaaggaag aagccgagga acgtctgaag gagacgaagg
     5041 tcttgtcgcc gctggacggc gtggtcgtcg cccggcacgt tcagataggc gaactggttc
     5101 agagcggcag gacctcgctg accggcggaa ccgttcttct ggaaatcgcc gacgtcagcg
     5161 aagtttatgc ggtggtgaac gtcgacgagg cggacatcgg ccaggtccgc cagttgttca
     5221 cggagacgaa ccccagagtg aaccccgcgg ccactcagtt ggcgactttg ccggagggta
     5281 ccatcgagac cgggcagaag gtcgacctga ctgtggaaac ctacccggcc gatgaggggt
     5341 tttacggcgt gatcgagcgg atctcgccgc agtcggaaat gagccgggcc atcgcgacct
     5401 tcaaggtctg gattcgggtc accagcccca accgtgacaa actgaagaag gtgttgaacg
     5461 cccaagtgca ggccaacttc accgcgaggt cggtcgctaa tgccttgctg gtcaactacg
     5521 aggccatgaa gcctgacccg agcggccaag gatacggcgt gtacgttccc gagaaagtgc
     5581 ccggcaccaa cgacgagaag ccgaagttcg tgccttgcga gttcggggtg gacaaccgcg
     5641 ttgacgtcga agtcaagaaa ggcctcgagg aaggccagcg ggtatacacg aagttgccaa
     5701 tcaagaccga gcgagagaag aagcgggagc ggcagcagtc tgaagaatag gcgatcagcc
     5761 tgtggctgat gttggtatga cgagatgatc cgcatcacga acctgacgaa gttctaccag
     5821 atgggcgact cggtggtgca cgcgctggac ggcgtggacc tgtcggtcga ggagggagag
     5881 ttcgtcagca tcacgggcgc ctccggaagc ggtaagagca ccatgatgca cctgatcggc
     5941 tgtctcgacc gcccgaccac cggagagtat gttctggccg gccagcgggt ggaaaagctc
     6001 agtgaccggc gtctggccgg gatccgcaac aaatacatcg gtttcgtgtt tcagacgttc
     6061 aatctcatca accgaacgac ggccatggac aacgtcgccc ttccgctggt ctacgcccgg
     6121 caggggcacg cccgacgtct tgcgtccgcc gcgctggagc gcgtcggctt gggccacaga
     6181 ggcgggcaca agcccagtga gttgtccgga ggcgaacggc agcgcgtggc catcgctcgt
     6241 gccttggtca acaagccgcc cctgctgctg gcggacgagc ccaccggcaa cctggactcc
     6301 aagaccgggg agcagatcat gagcctgttt cacgaactgc accagcaggg cgtgaccatt
     6361 gttctggtca cccatgagat ggcgatcgcg gcgcaggctc agcgggtcat ccgcatgaaa
     6421 gacggcaaga tcatcgagga ccgccgcgtg gacgaagctt tccgggaaca actgcttgcc
     6481 gaacgcatcg cggcgaccac ggcgcgactg accgaacaaa gcaggaggcc gccggccgca
     6541 cgatagcgtc ggctcgagat catgttcgca ctgcaaatca tgatcatggc tcttcgcgga
     6601 ctgcaggcaa acctgctgcg ctcgctgctg gccacgcttg gagtcatcat cggcgtgggg
     6661 gcggtcatct cggcgatgtc cattctggag ggcgcccagc gcgacatgat tgagcgattc
     6721 gagtcgctgg ggagtgagac gatcacggtc attcccaacg tggccaagag cggcggccgg
     6781 gcggtgggca tggccgaaac ccttgtcctc gaagacgccg actggattgc cgatccccgc
     6841 aactgtccca gcatcaaagc cgtggccccg gaagtatcgg gtttcgacgt gatcaagtac
     6901 ttcagtcgca acagcgaagt atcgatcctc ggaacgaatg aagattacgc cccgatgttc
     6961 aactaccgag tcgcgcaagg gcggttcctg acccgggacg acgtcgcatc gcaccagaag
     7021 gtcgcggtgc tcggttatca ggtcgcactc gacttgttcg gcagagcgga agccgtggga
     7081 cacacgattc ggctcaaagg ggcgtcgttc cgcgtcatcg gcgtcatgga aaagaagggc
     7141 aacatcggct ttcgcaccgt ggactcgcag gtcatcatcc ccatcaccac cgctcagagt
     7201 cggctcttcg gtatcaagtt catcaactcg atctcggccc aggccgtcga gaccaagctg
     7261 gttgaccagg ccatcgacga gatcaagggc gagcttcgcc gtcgccacga catccgcttc
     7321 cgcagcggtc aggaggacga ctttcgggtc ttcagccagg aggagcagcg gcgccagatc
     7381 ggtgagatcg tcttcctctt tcaggtggtt ctctacagca tcgccctgat cagcctcgtg
     7441 gtgggcggca tcgggatcat gaacatcatg ctggtctcgg tcacggagcg cactcgggag
     7501 atcggcgtgc gcatggccgt cggagcccag cgaagcgaca ttttgctgca atttctcatc
     7561 gaggccggca ccgtgagcct gctcggcgga gcgtttggcg tcggttgcgg cttcgcgttc
     7621 aataatctcc tggaggaaac gacccagatc atcgagacct atacgcccgc ccgggccctg
     7681 gtctgggcct tgtccatggc catcggcatc ggcattctca gcggcatcta tccggcctac
     7741 aaggcctcgc ggctggatcc ggtggaagcg ctacgttatg agtagcgttc atctgtcagc
     7801 aatcagcttt cagctctgag cgatccgggt tctgcgatct cgaatctgca atctcagact
     7861 tgagatctga gatccgagac ttgagatctg agatttgaaa tccgggttcg gccccctgac
     7921 cattcacatt agctcggggc ggaatcctct ccggacctgc catgccaacc gctcggcccg
     7981 gaatcctttc cgggcccaaa tgaggatggg aatcctccgc gaagaaggcc ccagaaagga
     8041 ttctggggcc agcggcgctg cgttatgagc ggctgactgc tgaccgctga atgctgaaaa
     8101 ctgacagctt atttgagccc caacacatcc atcatgtcgt atcgcccggc cggtttgccg
     8161 gccagccaga cagccgctcg cagtgctccg cgggcaaagg tgtcgcgggt gtgggccgaa
     8221 tgactgatgc ggacggtctc gccgaggttg ccgaagtgga cttcgtgctc gccgacggta
     8281 tcgccgacgc gcagtgcgtg catgccgatc tgacgcggcg ggcgccggcc ggtgtcgccc
     8341 ttacggccgt agatgacgtc tttgtcggcg tcgcggccgg tggccttgac gatcgaatcg
     8401 cggagtgaca acgccgtccc gctcggagca tcagccttga agcggtggtg agactcgacg
     8461 atttcgatgt cgtagtcgtc gccgaggatc gaggccacct ggccgacgag cttgaacagc
     8521 aggttcatgc ccacgctcat gttcgaggcc ttgaggacgg ggatagtctt agccgcttcc
     8581 tcgattgcgt tgttctgctc gggtgtgtgg ccggtagtgc cgatcacgat tgcccgcttc
     8641 ttcaccaagc agtaatcgag ccagtgcatc gtcccggccg ggcgtgaaaa gtcgatcagc
     8701 acgtcgaact cggtgtcggt ccggtcctgg agcggcaagc cggtcttgcc cagaccggcc
     8761 agttcgccga tgtccctgcc tatcgcgggg tgtccggtca tttccagtgc cgcctcgacc
     8821 tcgacatcct taaggtcctg agccagcgca acaacccgca atcccatctt gccggccgcg
     8881 ccggtaatcg ccacgccaat catgttctgc atctccgcaa gccagagtcg tcccggaacc
     8941 ggcgatgatt atctccgcgc gatgatctca actcaagtcg cacggcgggc tcgcggccgc
     9001 cgcagtggcc agacgcgcta ttggttttga atcgccaagt tatccatcgg tcgtgttctg
     9061 cttgctcgca acggacgact tgaagggcgt ccacctggcg gtagactgct ggcatggctc
     9121 gtctgagacg caacaggatc atgctcaagt ggcacccttc agcaatgcgt cgagcgctca
     9181 cggtgagcgc ccttatcatg tccgcgctgg ttgttgttgt gagaatcaga ctgattccgc
     9241 agttctggcg ctacaccgac ggcatctact tcgacacttt gctctggttt gtctgcatcc
     9301 caccgatcat cctcttgtgg ctcgacctgc gccgtgtctt cccgccggga cattgccagg
     9361 aatgcgggta tgacctcgcc ggcaagacca gcggcgtgtg ccccgagtgc ggggagacga
     9421 tcagcgaacg gccggctcca gagaagcggc atttgagtcg ggctcgactg gggcgcacgg
     9481 gccggatgct gggacgcaaa ggccgggtgc tcaaatggac gggagcagcg acctgtaccg
     9541 tcatcttcct cgcatgggca gcgtccgttc cgctgcaatt tggatatgta tacgcctatg
     9601 gcgcgcccgg cagtcgacaa ggctcctatc acatccactt caacgatgga aggatcaaca
     9661 tttctcacct caaccaccgt tcgccttacg gttcctatgg gtattgccag cgtcgttctg
     9721 aaagtgcaga gatttcgccg gtttgaatat gtagtct
//
LOCUS       DAOTPW010000222         9746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6437_length_9746_cov_6.359922, whole genome shotgun sequence.
ACCESSION   DAOTPW010000222 DAOTPW010000000
VERSION     DAOTPW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6437_length_9746_cov_6.359922"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="P5316_17115"
     CDS             complement(<1..1219)
                     /locus_tag="P5316_17115"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRR86733.1"
                     /translation="MSEHLKAQSPARLSSLQMSMLILLRLAVGWHLLYEGAGKLMAPG
                     WSAEGYLSHSNWILAPLFRWMAADPGRLRVIDALNIWGLILIGLALLLGVMPRLASLG
                     GAVLLLLYYAAISPLYGPGVFGAESDYFGVSKVLIEAIALLTLAAMPSGSMFRLDRLV
                     AGFIRPRHPGRVGSGGMGPSGDETVESQSRSSLHRREILGSLASLPLVGLFCAGLYQR
                     RRRDRAVVQAITGATIKVPTADLSKLKGKPPMGQIGSLKISRLMLGSNLIGGWAHSRD
                     LIYVSSLFKAYNTEWRIMHTLALAEKAGINMINCVNPQLALIDKYRRQTGGAMQTMCQ
                     IRTVQGDMKTEIDMAIDAGATTMYIQGAVGDKCVEEKRLEELADAVAYGRRQGYLVGI
                     GAHSIRVPIACEEA"
     gene            complement(1514..3412)
                     /gene="argS"
                     /locus_tag="P5316_17120"
     CDS             complement(1514..3412)
                     /gene="argS"
                     /locus_tag="P5316_17120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRR86734.1"
                     /translation="MRSIAGQLNSLFSKAIERAFGDPGRRADPMIRPAADERFGDYQS
                     NVAMGLAKRLGSKPREVAQRIVDALAAEPQATAMCEPFEIAGPGFINIHLRPEWMADV
                     LNAVPAAGDRDRLGIDTAENPQTVVVDYSSPNIAKQMHVGHLRSTIIGDSIARILEFE
                     GHKVIRQNHIGDWGTQFGMLCAYLKEKMPDALSNPADVHLADLESFYKQANALDQEDA
                     AFHERARAEVVALHNRDQSTLKAWRYIVNESRNHYMPIYRRLGVSLRQEDERGESFYA
                     DRLAGVVAELERDFGQGAPSRPLDEPRGSSPRSDLPRIRVEVSDGALCVFHETPSGEP
                     MFKNPDGKPVPMIIRKSDGAYLYATTDLAAVQFRIRELGADRIVYVTDARQAQHFEMV
                     FATARAAAWTLHGGRTTPVQLDHVTFGSILGDDRKPLKTRSGENVRLADLLDEAVNRA
                     EKLIRSNEADPAKRRGFTQDEIRDIAEAVGIGAVKYADLSQNRQSDYVFSWDKMLAME
                     GNTAPYMMYAYARIRSIYRKGAEESRDLAAGRVQLVEPAERVLARQVLRLPETLEGAA
                     VGLRLNIITEYLYNLAGAFMSFYEACPVLKAGSDALRASRLRLCDLTARALRIGLDLL
                     GIRVVERM"
     gene            complement(3877..4413)
                     /gene="folK"
                     /locus_tag="P5316_17125"
     CDS             complement(3877..4413)
                     /gene="folK"
                     /locus_tag="P5316_17125"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRR86735.1"
                     /translation="MSDQPVDAFIAVGSNIRPQQNVTDALELLQQQVRVLASSTFYWT
                     SPLDRTDQPRFLNGVWRIRTSLLPRSLKFDVLRGIESKLGRARTKDKYAPRPIDLDIV
                     LYGRLMLNEPDLLIPDPDIRRRPFIAVPLLELAPDLTMPDTGEPIAALADRQTAMHLE
                     PAEAVTKRLRQAIRTRHA"
     gene            complement(4406..4789)
                     /gene="folB"
                     /locus_tag="P5316_17130"
     CDS             complement(4406..4789)
                     /gene="folB"
                     /locus_tag="P5316_17130"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRR86736.1"
                     /translation="MASDGDRLDHIHIRDLALRCVIGIYEEERREKQDVIINITLYAD
                     LHKPCRTDDVADTIDYKVLKKKIIALVEQSSCRLIEHLAEGVAQTCLAEPRVVRVTVC
                     VDKPAALRFARSVAVEITRDRESRV"
     gene            complement(4779..5519)
                     /locus_tag="P5316_17135"
     CDS             complement(4779..5519)
                     /locus_tag="P5316_17135"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR86737.1"
                     /translation="MDLSGKTALITGGGKRLGRATALALASQGVSVVVHYQTSTQAAE
                     QCADAARRLGVNAWTIHADLGDTAVCEKLFDRAGKQAGSIDILVNNASIFPRNTLLDM
                     TVPDVAENVQVNAMAPFLLSRSLAAQGRPGAIINLLDTRVVHYDRVHAAYHLSKRMLL
                     TLTRMMALEFAPSIRVNAVAPGLILPPPGEDESYLQKMAAGVPLRRMGSVEEITAAVL
                     FLLRSDFITGQVIFVDGGFHMKGSVYGL"
     gene            complement(5623..6495)
                     /locus_tag="P5316_17140"
     CDS             complement(5623..6495)
                     /locus_tag="P5316_17140"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HRR86738.1"
                     /translation="MKELVSPKQVALALGVSEASLKRWCDKGLIPAIRTAGGHRRLPI
                     NGVIHFIRQTGQHVARPEILGLPPITRQTAAAIESLKTLFRDALESGDEDQARRIVMS
                     MFVNGRKAHEICDSIIAPSFHEIGDRWQHGEIEIYQERRGVEIVSRLLHELRLILPPP
                     PEAAMTAIGATLQGDPYGLATSMVEVTLLEAGWAARSYGCGHPAATLAAAIEDVRPSL
                     FWLSASVIREIPVFIQECRLLYETALSCGSAFVIGGRALVPEIRCQIGYSAFCDTLAH
                     LWGLAMALRPRGGG"
     gene            6736..7374
                     /gene="folE"
                     /locus_tag="P5316_17145"
     CDS             6736..7374
                     /gene="folE"
                     /locus_tag="P5316_17145"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRR86739.1"
                     /translation="MIAQKNVVESLVRFPSLSARHGSTQRHSDRKVEKAVRQLLEALG
                     EDPDREGLADTPRRVARMYGELFAGLRTDPAVHLQRVFNENYDEIVLLRNINFFSLCE
                     HHLLPFHGVAHVAYLPNGKVVGLSKLARTVDAFARRPQVQERMTAQIADALMEHLAPK
                     GAVVVVEAEHLCMKMRGVQKPNGTMVTSAVRGTFRDHAAARAEVMGLINGGP"
     gene            7561..8592
                     /locus_tag="P5316_17150"
     CDS             7561..8592
                     /locus_tag="P5316_17150"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR86740.1"
                     /translation="MFQRSGPALVLLAALVASSAGCALPMVLPVAVPRGEIETKTGIT
                     FAEAGGETLKLDVARPTTGKGPWPAVVCIHGGGWVSGCRMSMRPWIEYLAVYGYVAVA
                     PTYRLAPRHPFPAAVADVRNCVRWLRRHAKEYNIDPDHIGAMGLSAGGHLSLMLGLAS
                     DDDRFGPEDEAETGESARVQAVVNYFGPGNLVPRSWSEFAVRQYLVPFLGPDAKQREE
                     KARRASPLSYISNDDPPVLTFHGDADSLVPPPQAYELHSRLSQAGLSNELQILHRQDH
                     GWGEPHLSRTRLDMIRFFDRHLKGPKAGRLLRAATQAHCDPETCAQAAPISGRKVLIT
                     CPATATAPE"
     gene            complement(8893..>9746)
                     /locus_tag="P5316_17155"
     CDS             complement(8893..>9746)
                     /locus_tag="P5316_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86741.1"
                     /translation="KAPGQTVVNGQWAHVVISRTGATAFATASGNLEIRLLADDKEGG
                     AQTVAWDNLSITVTDPNQGNAVVWTYSDDFESGLAGWTQMVRPNDSHDWQDPWWLGNK
                     VYVFTDDLYDQNNPDIFYNAQFQVARWPGDNDSDGTPEPEQPWNGIWWRKVIERPFTT
                     TTGNIDVRLLTRQKYAGKDSLEGYAPEVAAWDNVKLSFILPCNPVRFDADDDGDVDQA
                     DFAVLQACYTGADDPDEIFNAEACRCVNSDGDSDIDAEDVAAFEACASGPGVLANVNC
                     DNALSPP"
BASE COUNT         1792 a         2855 c         3206 g         1893 t
ORIGIN      
        1 ccgcctcctc gcacgcgatg ggcacgcgta tggagtgggc accgatgccg acgagatagc
       61 cctgtcgccg gccgtatgca accgcgtcgg cgagttcctc gagccgcttt tcctccacgc
      121 acttatctcc caccgccccc tggatgtaca tggtggtggc gccggcatcg atggccatgt
      181 cgatttcggt cttcatgtcg ccttgcacgg tccggatctg gcacatggtc tgcatggcgc
      241 cgccggtttg gcggcggtac ttgtcgatga gagccagttg cggattgacg cagttgatca
      301 tgttgatgcc cgccttctct gccagggcga gggtgtgcat gatcctccac tcggtgttgt
      361 aggctttgaa cagcgaggac acgtagatca gatcgcgtga gtgggcccac ccgccgatca
      421 ggttgctgcc gagcatcaac cggctgatct tcaagctgcc gatttgcccc atcggcggct
      481 tgcccttgag cttgctcagg tcggcggtgg ggaccttgat ggtcgcgccg gtgattgcct
      541 gtaccacggc gcggtcccgg cggcgtcttt gatagagccc cgcgcagaac aagcctacca
      601 gcggaagcga agccaagctc ccgaggattt cccttcgatg caaagaagac ctggactgcg
      661 actcgacggt ttcatcgccg gaaggcccca tgccgccgga gccgaccctt cctgggtgtc
      721 gaggccgaat gaacccggcc accagccgat cgagtcgaaa cattgaaccg gacggcatcg
      781 cggccagcgt caggagcgcg atggcctcga tcaagacctt gctgacgcca aaatagtcgg
      841 actcggcgcc aaacacgccc ggcccgtaca gcggcgagat ggccgcatag tacaacagca
      901 gcaacaccgc tccgcccaga ctcgccagcc gtggcattac gccgagcagc agcgccaggc
      961 cgatgaggat aaggccccag atgttcaggg cgtcgatcac gcgcagtcga cccgggtcgg
     1021 cagccatcca gcggaagagg ggagcgagga tccaattcga gtgcgacaag taaccctcgg
     1081 ccgaccagcc gggggccatg agtttgccgg ctccttcgta cagcagatgc catcccacgg
     1141 ccaagcggag aaggatgagc atggacattt gcagtgagga caaccgggcc gggctttggg
     1201 cctttaagtg ttcgctcatg attcagacac tcctcgcacg gggatgaatg aacggcgacc
     1261 agcataccat agagtaaggc cgtgtagcga gcggcgtccg gcacgcgcgg cgggtctgag
     1321 tcctgctccg accgtgccgt gcctttgggc cacctccgtt ctcattcgcg ggcagcaccc
     1381 atggcaggtc tcgccgcgtt tggcggcttc gaccgtgccg tgccttgtgg tggcaatcgt
     1441 tacacccgct ccggcgcgca gacgccggac aacctggtgc agcgacgggg gcgtcggccc
     1501 cacttgggag ccgtcacatt cgttcgacga cgcggatgcc gagcaggtcg aggccgatgc
     1561 ggagggcccg ggcggtgagg tcgcagagcc tcaggcgcga ggcgcgaagg gcgtcggagc
     1621 cggccttgag gaccgggcag gcctcgtaga agctcatgaa ggctcctgcc agattataca
     1681 ggtattcggt gatgatgttg agccgcaggc cgacggcggc gccttcgagg gtttccggca
     1741 ggcggagcac ctggcgggca aggacccgct cggccggttc gacaagctga acacggccgg
     1801 ccgctagatc acggctctct tcggcgccct tgcgatagat cgagcggatg cgggcgtagg
     1861 cgtacatcat gtacggggcg gtgttgccct ccatggccag cattttgtcc caactgaaga
     1921 cgtaatcgct ctggcggttc tgcgagaggt cggcgtactt gaccgccccg atgccgacgg
     1981 cctcggcgat gtcgcggatt tcgtcctggg tgaagccgcg gcgtttggca gggtcggctt
     2041 cgttggagcg gatcagtttc tcggcccggt tgacggcctc gtcaagcaga tcggcgagtc
     2101 tgacgttctc gccgctgcgg gttttgagcg gcttgcggtc gtcaccgagg atcgatccga
     2161 aggtcacgtg gtcgagttgc acgggcgtcg tgcggccgcc gtggagcgtc caagcggcgg
     2221 cccgggcggt ggcgaagacc atctcgaagt gctgggcctg acgggcgtcg gtgacgtaga
     2281 cgatgcggtc ggcgcccagt tcgcgaatgc ggaattgcac ggctgccagg tccgtagtgg
     2341 cgtagaggta ggcgccgtcg gacttgcgaa tgatcatcgg cacgggcttg ccgtcggggt
     2401 ttttgaacat cggctcgccg gacggggttt cgtgaaagac gcagagggcg ccgtcggaga
     2461 cttcaacgcg aatacgtgga agatcggacc gcggggatga accccgcggc tcgtcaagag
     2521 gccgggatgg agcaccttgg ccgaagtcgc gttcgagttc ggcgacgacg ccggcaagtc
     2581 tgtcggcgta gaagctttcg ccgcgttcgt cttcctgacg caggctgaca cccagccgcc
     2641 ggtagatggg catgtagtgg ttgcgcgact cgttcacgat gtaccgccag gccttcaggg
     2701 tactctggtc gcggttgtgc agagcaacga cctcggcccg agcccgctcg tgaaaagcgg
     2761 catcttcctg atcaagggcg ttggcctgtt tatagaagct ctccaggtca gcgaggtgaa
     2821 cgtccgccgg gttgctcagg gcgtccggca tcttctcttt gagataggcg cagagcatgc
     2881 cgaattgcgt cccccaatcg ccgatgtggt tctggcggat gaccttgtga ccttcgaact
     2941 cgaggatacg ggcgatggag tcgccgatga tggtgctgcg gaggtggccg acgtgcattt
     3001 gcttggcgat gttcgggctg gagtagtcga cgacgacagt ttgtgggttc tcggccgtgt
     3061 cgatgccgag gcggtcgcgg tcgccggcgg cggggacggc attgagaacg tcggccatcc
     3121 actcaggccg caggtgaatg ttgataaagc ccggcccggc gatctcgaag ggctcgcaca
     3181 tggcggtagc ctgcggttca gcggccaagg cgtccacgat tctctgggcc acctcgcggg
     3241 gcttggaccc gagccgcttg gccagaccca tggccacgtt gctctggtag tctccgaacc
     3301 tttcatcggc ggcggggcga atcatcggat cggccctgcg gccggggtcg ccgaaggccc
     3361 gttcgatggc cttggagaac agcgaattca gttgtccggc gatcgacctc atgacgttct
     3421 ccatgctgtt gcggcgtcgc aaagggcggg attctagcac aaccgggcat cgaatcgaac
     3481 ctggtcaggc cccaggcagg cgccctccgc aacgggctgt ttttcacata aagtctttta
     3541 tagtaaagac ttaggtggaa acggcgttgg gttcgtttgg cgcggcgagc tttttggtgc
     3601 tgccggacaa acgccctttc ggccaggcga cagccgtctg ttcgtgggca gagtatctcc
     3661 ctgccgcttg cctcacgagg ataacacacg gggtgctccg gaaggaaagc gaataggcga
     3721 ttttggggtg tctgggccgg gcaaaacggg atcgcgccgc caagccgttg gccatagaca
     3781 ggcaccggcg agcgaggtta ccagacaatc gttgtttcgg gttgaggtag ccgcagggct
     3841 gggggccagt tcgaaggctg agtgcgtcgg gtaccgttat gcgtgccggg ttctgatcgc
     3901 ttgccggagg cgcttggtca ccgcctcggc gggttcaaga tgcatggctg tctgccggtc
     3961 ggccaacgct gcgatgggtt cgcccgtgtc cggcatggtc aggtcggggg ccagctccag
     4021 gagcggcacc gctatgaaag ggcgccgacg aatatcgggg tcggggatca acaggtcggg
     4081 ctcgttcagc atgagtcggc cgtaaagaac gatgtcaaga tcgattggcc ttggggcgta
     4141 cttgtccttg gtgcgggcac ggcccagttt tgactcgatg ccacggagga cgtcgaactt
     4201 cagcgatcgg ggtaagaggc ttgtccggat gcgccagacg ccgttgagaa aacggggctg
     4261 atcggtccgg tccagggggg acgtccagta gaacgtggat gaggccaaga cgcgaacctg
     4321 ctgctggagc agctcgaggg cgtcggtcac gttttgctgc ggcctgatgt tcgagcctac
     4381 ggcgatgaag gcatccacgg gttggtcaga cacggctttc tcgatcgcgg gtaatctcca
     4441 cggccacgct gcgggcgaaa cgcagcgccg ccggcttgtc gacgcagacc gtgacgcgca
     4501 ccacgcgcgg ctccgccaag caggtctggg cgacgccctc ggccaggtgc tcgatcagtc
     4561 ggcaggatga ctgctcgacc aaggcgatga tcttcttctt aagcaccttg taatcgatgg
     4621 tatccgcgac atcgtcggtt cggcacggct tgtgcagatc agcataaagc gtgatgttga
     4681 tgatgacgtc ctgcttttcc cgccgttctt cctcgtagat gccgatgacg cagcgcaggg
     4741 ccaggtcgcg gatgtggatg tgatcgagac ggtcaccatc agaggccata cacgcttccc
     4801 ttcatgtgaa aaccgccgtc gacaaagatc acctggccgg tgatgaaatc gcttcgcagc
     4861 agaaagagga cggctgcggt gatttcctcg acgctgccca ttcgccggag gggaacgccg
     4921 gcggccatct tctggagata ggactcgtct tcgcccgggg gcgggagaat gagtccggga
     4981 gccacggcgt tgacccggat cgacggagcg aactccaggg ccatcattcg agtgagcgtc
     5041 agcagcatcc gtttgctcag gtgataggcg gcatggacgc ggtcgtaatg gacgactcgg
     5101 gtgtcgagga gattgatgat cgcgccgggg cgtccctggg cggcgagcga ccgagaaagg
     5161 agaaagggcg ccatcgcgtt gacttggacg ttttccgcga cgtccggcac cgtcatatcg
     5221 agcagggtgt tccgcgggaa gatcgatgcg ttgttgacga ggatgtcgat cgatccggcc
     5281 tgtttcccgg cccggtcgaa cagcttttcg cagaccgccg tgtcgcccag gtcggcgtga
     5341 atggtccaag cgttgacacc gaggcgtcgg gcagcgtcgg cgcactgttc ggcggcctgg
     5401 gtggaagtct gatagtgcac gaccacggat actccctggg atgcgagggc gagcgccgtc
     5461 gcccgcccga gccgcttacc gccgccggta atcagtgccg tcttgccgga gagatccatg
     5521 gctgaacgtg cctccagaaa acctgcgata caccttatgg tgtcaggcag gctttggcaa
     5581 acggaacgat ggtcagccgg gccaggttaa ccaagtattg catcaaccgc ctcctctcgg
     5641 tcgcaaggcc atggcaagac cccacaagtg agcgagggtg tcgcagaacg ccgagtagcc
     5701 gatctggcat ctgatctcgg ggaccagtgc tcgaccgccg atgacaaaag cacttccgca
     5761 gctcagggcc gtctcataca acaagcgaca ctcttgaatg aaaacgggga tctctcggat
     5821 aacggacgca ctcagccaga acaagctcgg ccgcacatcc tcgatggccg ccgccaaggt
     5881 agccgccgga tgaccacagc cataggacct cgccgcccaa cccgcttcca gcaaggttac
     5941 ctcgaccatg ctcgtcgcga gcccgtaagg atcgccctgc aatgtggcgc cgatcgcggt
     6001 cattgccgcc tcgggaggcg ggggaagaat cagccgcagt tcgtgaagca gtcgcgacac
     6061 gatctcgacg ccacggcgtt cctggtagat ctcgatctcg ccatgctgcc accggtcgcc
     6121 gatttcgtgg aaggaagggg cgatgatgga atcgcagatc tcatgagcct tgcgcccgtt
     6181 gacgaacatg ctcatcacga ttcgtcgagc ctgatcctca tcgccagact cgagggcatc
     6241 tctgaacagg gtcttgaggc tctcgatggc ggcggcggtt tgccgggtga tcggcgggag
     6301 gccgaggatt tcgggccgag ccacgtgctg acccgtctgc cggatgaaat ggatgacgcc
     6361 gttgatcggc aatcgccggt ggccgcctgc ggtccggatc gcgggtatca atcctttatc
     6421 gcaccagcgt ttcaaagacg cttcgcttac gcccagtgcc agcgcgacct gctttggact
     6481 gaccagctct ttcatggcat cacccctgat gaacgttttg aacgattcta ggccattttc
     6541 gagatttggg tagttactcg gctaccacga actgactgag ttgatcggcg attgcgatct
     6601 aagttatttt atgaaaatgg tttaataagc gcacaaccca aatgaacgat ttgaacgatt
     6661 ttctgctggt catccgtgat ttggtagcct attctctggc gttggccgca aggtagcggc
     6721 ctgggaggat gtcagatgat cgctcagaag aacgtggttg aatcgctggt caggtttccg
     6781 agcctctctg caaggcacgg ttctactcag cggcactcgg accggaaggt ggagaaagca
     6841 gtgcgtcaac tgctagaggc tcttggtgaa gatccggatc gcgaaggtct ggccgatacg
     6901 ccccggcggg tggcgcggat gtacggggag ttgttcgcgg gtttgcgaac ggatccggcc
     6961 gtgcacctgc agcgtgtgtt caacgagaat tacgacgaga ttgtgctact gcgcaacatc
     7021 aactttttca gcctctgcga gcaccatctt ctgccctttc atggtgtggc ccatgtggcg
     7081 tatctgccga acggcaaggt ggtcggcctt tcgaagctgg caaggacggt tgacgcgttc
     7141 gcccgtcggc cacaggtaca ggaacgtatg acggcgcaga tcgccgacgc gctgatggag
     7201 catcttgccc ccaagggggc ggtggtggtt gttgaggccg aacacttatg catgaaaatg
     7261 cggggggttc agaaaccgaa cgggacaatg gtcacctcgg cggtgcgggg gacgttccgg
     7321 gaccatgcgg ccgcccgtgc cgaggtgatg gggttaatca acgggggtcc ttgagtttcg
     7381 tttgcttgct caacgttacg tttgaggagt caggggggaa tatgacggcc tgcaacggcg
     7441 ggcgattctg tgaagtatct ccattggggc ccgagtcagc cgccaatagg agcaacgcgc
     7501 tgcgccattg tctcatcatg agacttgacg tccgcgatcg aagcaactac agatggcggc
     7561 atgtttcaac ggtcaggacc agctctggtt ctgctggctg cgctggtcgc gtcgtcagca
     7621 ggttgtgctt tgccgatggt tcttccggtg gccgtgcccc gcggcgaaat cgaaaccaag
     7681 acgggcatca ccttcgccga agcgggcggc gagacattga agcttgatgt ggcgcggccg
     7741 acaaccggta aaggtccgtg gccggcggtg gtatgtatcc acggcggagg atgggtttcc
     7801 ggctgccgca tgagcatgcg gccgtggatc gagtatctcg cggtctacgg ctacgtcgcc
     7861 gtcgctccca cataccgtct ggcaccgaga caccctttcc cggcggccgt ggccgacgtt
     7921 cgcaactgtg tccgatggct gcggcggcat gcaaaggagt acaacatcga ccccgaccat
     7981 atcggggcca tgggtctgag cgccggcggg cacttgtcgt tgatgctcgg tctggcgtcc
     8041 gacgacgacc ggttcggccc ggaggacgag gccgaaaccg gcgaatcggc acgggtgcag
     8101 gcggtcgtca actacttcgg accgggaaac ctggtaccaa gaagctggtc tgagtttgcc
     8161 gtccggcaat acctcgtacc gtttcttggg cctgatgcga aacagcggga agagaaagct
     8221 cggcgagcct ctcctttgag ttacatcagc aacgatgatc ctcctgtact cacatttcac
     8281 ggggatgctg attcattagt tccgccgccc caggcttacg agcttcacag tcggctgagt
     8341 caagccgggc tgagcaacga gctgcagatc ttgcaccgcc aggatcacgg ctggggcgaa
     8401 cctcatctga gccgcacccg cctggacatg attcgcttct tcgatcgtca cctgaaaggg
     8461 cctaaggcgg gccgcctttt gcgggccgca acccaagcgc actgcgatcc agaaacatgc
     8521 gcgcaagccg caccgatatc cggccgcaag gttctcatca cctgtccggc cacggcgacc
     8581 gctcctgaat aggagacagc ccaggatcat tctccatttt tcgtgctgtt ctcgcggacg
     8641 acgagaaggt tgcgttgtcg attctgggac ccaacccttc gggaatgggt acctgcgcct
     8701 cgcgttgtcg atttggccgc ccagccgttc gggccaggcg actcggccca ttggagtggg
     8761 gcaccggcca ccggcgcgag caacaaaaac gggcaaggcc catcctccgc tgagggacgg
     8821 gccttgccga ctcacatcga cgtccggacg ccccgtgcgc cggacgcagg gtcatggcac
     8881 acctgccggc cattacggcg gcgacagcgc gttgtcgcaa ttgacgttgg cgaggacacc
     8941 cgggccggag gcacacgcct caaaggcggc gacgtcctcg gcgtcaatgt ccgaatcgcc
     9001 gtccgaattc acgcagcggc aggcctcggc attgaagatt tcatccgggt catcagcgcc
     9061 cgtgtaacac gcctgcagga cggcgaagtc ggcctggtcg acatctccat cgtcgtccgc
     9121 atcgaaacga accgggttac agggcaggat gaatgacaat ttcacgttgt cccacgccgc
     9181 aacctcggga gcataaccct ccaaggagtc tttgcccgca tacttctgac gagtcagaag
     9241 tcggacatcg atgttgccgg ttgtggttgt gaacggccgc tcaatgacct ttcgccacca
     9301 tatgccgttc cacggctgct cgggttccgg ggtcccatcg ctatcattgt ccccaggcca
     9361 gcgggcaacc tggaattgag cgttgtaaaa gatgtcaggg ttattctggt cgtacaagtc
     9421 gtcggtaaag acgtagacct tgttgccgag ccaccaggga tcctgccagt catgcgaatc
     9481 gttgggccga accatctgtg tccagccggc aaggccgctc tcgaaatcgt cggaatacgt
     9541 ccaaacgacg gcgttaccct ggttcgggtc ggttacagtg atgctcaagt tatcccaagc
     9601 gacggtctgg gctccgcctt ccttgtcgtc ggccaggagg cgaatctcca gatttcccga
     9661 ggcggtggca aaggcggtgg ccccggtcct ggagatgacg acgtgggccc actgcccgtt
     9721 cacgaccgtc tggccgggag ccttgt
//
LOCUS       DAOTPW010000223         9587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6567_length_9587_cov_5.707721, whole genome shotgun sequence.
ACCESSION   DAOTPW010000223 DAOTPW010000000
VERSION     DAOTPW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6567_length_9587_cov_5.707721"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..2536
                     /locus_tag="P5316_17160"
     CDS             35..2536
                     /locus_tag="P5316_17160"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRR86742.1"
                     /translation="MHTFARLVMTLGGAVSLGCSQLPQHPELVPPPQRIQWAAQPPLE
                     ISRDSVTIVLGTRATAPEKHAAQLLQDSVSRRFRQEWPIGEESRLPAGCETAILLGRR
                     SSSEWLERLCRQKQINLRTAAPGHDNYIIEILDDDNRKVVVVGGGNPRGVIYGQDTLF
                     QMLRERAGRLELVRASVCDWASIPWRGRPQTNVELYLRPGELDCYLRSRINFVDLRNG
                     IYAFEPSDTLEETLLSQAINEIHRRGMLVYGTVNTGVPETEYDKAIAMFERFIALGAD
                     GLWVSFDDKGPGDNPEEIVRRVLELGRKHGITGERIAITPPKGAYQDIAAPFNRKIMA
                     VPGMESALWFWTRYPCDSDVEAARSIGLKVLPSWWHNYPRTLNSHDYTGIPPLAMGWH
                     GPGYELLADAGRQFTAVMPWGGNAWGRYYVVPVIGWWGWNPKAHDWQATRTRIHGFVF
                     GESQTAAAAEFDETLARIRPLLHYAVGDGEWQPAGPPRLKNPADRRRGLALVDQMEAL
                     LTKIKHAAPVQSMLDPDQLQAGYLSKMEKEVATLRAAATLPFPEYWWIAHQRAVLDAV
                     YDDDMARVERLTAAVREPLLRDIDEINQKGRQLDKIDDYVKWWTEQANLDAAGWKNLV
                     EKRRKELANRLADYNYFVKVRADMLKGLADPPVDWGTGRWEYANLVRATATFTQREYY
                     WGNWIAGLCDHEGGKVAAFATSRRTPAADGEFAELELALPISGDRSRLALLIYLADVN
                     KELIGLHYVRWRWAGYRRIQLLHRDHVLWEADIGVQREQGEWFMVKLPPVPETVKQVT
                     LRLRVENLRGAIGNQTLALVGPIRLIELPMRTNVD"
     gene            2555..4948
                     /locus_tag="P5316_17165"
     CDS             2555..4948
                     /locus_tag="P5316_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HRR86743.1"
                     /translation="MNMRFACLVIPLVSWSAALAARIAPPIRAQTTPMPAAIQAGQAV
                     DIVLDRYWRGNVKVDQDGLHDNKKTFPHVPHLIEYRFELPVGGEYELQGRYVAETDAP
                     TYLTIDGKLQGMRFEEAGDHRSKWVSLAKTPLTPGKHWIRFTSQYVETPFPTLTGLRL
                     VFHGGPGPEPEPEPPIVGPRPSLPDDWSKTISRKIHSDFHTAGFIRGIGSRFDGTTFG
                     KTLRDNGVNAICIFAKGHHGYAYYDTKVGTRHPGLDFDLMKAQIEACRRYGIAVWTYF
                     SIAPDELYTSTCDQTITDPNDRPTDMEVDVESPYVADYLWPMIAECVRNYDLDGLFFD
                     FPGNEEFVRETVRLVKRIKPGMVVAYNHQWAKSRDELGELDVLELESWRHKQPLYHWQ
                     YYARYARGAVPLTAMTIRFHKSWGDFGGITSEAMLRVHAATAMANGCLLTIGDHLHPS
                     GQLDPAVYERIGRVLRDVMRIEPYVTGSESLPYVALMRPKEIALMGADNPCHALLDSG
                     IHFTVIDPSQDLKPFTAVLIPEAKMADDALTARLKEYVENGGRLLAFGKPPARMAELL
                     GIEAQPANEAAYIRIDPRILPTPPATVLYTYLEVAPARPLEDTTTLAPLVWAMNHGTI
                     HTSRRQSPPSDEPSGLAAITSRKLAKGQAVYCAALLPEVYARWGYSAVREIVADLLRY
                     MIPPDKRLADVEAPVHLEVSLNRQGNRVIVHLVHSPQSRASLGTFNKDDLVNQDPVID
                     EMPTVAGTRLRLAESLVGNRSIKLLPAGTEIRPDSRANGVVTIRVPDFQISSVLLIE"
     gene            4976..6139
                     /locus_tag="P5316_17170"
     CDS             4976..6139
                     /locus_tag="P5316_17170"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86744.1"
                     /translation="MRVHRLAMVGCGAFARLYHLPIIHANPRAQLAVVCDLNPDVAKE
                     CAERFHARKATADWHEVIDDPDVDAIILATHTNLRAELIIPALQAGKPVFVEKPVANT
                     DAEMVEIVRAGRAARLPVCVDHNRRSSPAMHDLCQLLQKARNGPGGHLPSVDRSDGGK
                     RAAMPQEKQTQILIRVNDDCRSWVDWALTDAEGILFAEMVHFIDVAMWLMNPLPITRV
                     FAEGSAQGNFALIMRFADGSLATIQQTICGHFDYPKELIEVTANHVTMAMEHHVELRQ
                     RGLEDEPFRRTYPFKSIDGGIEDGGIEAFHRAVTETFDNARRTGNPPVFISPDKGHAA
                     HIDRFLDCIEGKGENPCDVVDAVRVTRVALKLLESVRREQPVEIRDSDWQIEA"
     gene            complement(6362..6628)
                     /locus_tag="P5316_17175"
     CDS             complement(6362..6628)
                     /locus_tag="P5316_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86745.1"
                     /translation="MRGEHLLKGFSNRDIRHHLGLTTPNKVESRRQAAYVSRLLKRLH
                     LHGLIAKIQRSRRWRLTHKGQVLMTAVLMLHDRHYPQALHNHAA"
     gene            complement(6618..6833)
                     /locus_tag="P5316_17180"
     CDS             complement(6618..6833)
                     /locus_tag="P5316_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86746.1"
                     /translation="MERFIDKHQAQITGTISCSDRILFKGHLLLGRPEAMEQFIARQG
                     ILIKDFGTFVTRGLPPRRPSVRRGDAR"
     gene            complement(7103..9481)
                     /locus_tag="P5316_17185"
     CDS             complement(7103..9481)
                     /locus_tag="P5316_17185"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HRR86747.1"
                     /translation="MLLHKCLNHAALFSLTLLAACGRPAAELRFDPQQRAGLHAIEMN
                     KPAQDFFEGAVLGNGGMGVIVCTRPDSVMIHLGHNGVWDIRVAENNREKLATFDELWA
                     RLKRGMPEDKAWFDDYCKMARENYAQPYPRPWPCGSVLLGFDRRKAELLGHRVRIDTG
                     RCEVEFLIDGRTVTLVLFNEMNADRLWMRMVDAEGRPIQAPFDRVRLIPEKGMATQTA
                     STADTLSFRQVLPVLGDDRDKDKALRVAVRVNGRIGAPLVQDGPFVACVQLEHGMARV
                     LSKPEAEVPEPSDDAFAAASAEAAGAWKDYWSRSGVQLDDEFLERTWYRNMYFLNCAV
                     RPGEVCPGLFANWSYGNIGTAWHGDYHTNYNLQQPFWVTFSSNRVEKHLPYVDLVDFL
                     LPVSREWAKNHYKLPGAFFPHSAYPVEMTMMPYPVPTWGAEVCETSWVVQSLWWHYLY
                     TMDKEFLRDRAFGPICEAVQFLNAYMRRPDAHGPQWNDDKYHIYPTVVPELHGLKVDP
                     AFSADCIVDLTLTKFVFKAYLEACKVLGRDREETELARQVREILDHFPEYITAQSQRG
                     KVFVSVSGESPEQVYNTPNPLMPVFPGEDIGLHSPKEVLEIALNTWRNQRNEGGNELV
                     FLNLQGARLGVLDLERFKRQISYCLMPNGTCADMVLQVGGRYSDETDYAFMARMGIWF
                     ENFSLPVVVNECLLQSYTGQLRFFPNWPRDKDAAFQDLRAVGAFLVSAEMKNGRVSWI
                     RVRAEVGGPLRIINPWPGKVEVIRGDATETMSGELLELETQAGETLIFRARN"
BASE COUNT         1944 a         3084 c         2823 g         1736 t
ORIGIN      
        1 tcaggcgcat tcctcaaatc aacaggagtc cgacatgcac acgtttgcca ggcttgtgat
       61 gactcttggg ggcgcggtct ccctcggctg ctcacagttg ccgcaacatc ctgagctggt
      121 cccgccaccg caaaggattc agtgggcggc ccaaccgccg ctggaaatca gccgggacag
      181 cgtgacgatc gtgttgggaa cccgggccac cgcccccgaa aaacacgccg cccagttgct
      241 ccaggacagc gtgtcgcgac ggttcagaca agagtggccg atcggcgagg aaagccggct
      301 ccccgccggc tgtgagacgg ccatattgct cggccggcgg tcatcgagcg aatggttgga
      361 acggctttgc aggcagaagc aaatcaacct gagaacggca gcccccggcc acgacaacta
      421 tatcatcgaa atcctcgatg acgacaaccg aaaggtcgtc gtggtcggcg gcggcaaccc
      481 gcgcggcgtc atctacggcc aggacacgct tttccagatg cttcgggaac gcgccggacg
      541 gttagagctg gtccgagcat cggtgtgcga ctgggcaagc attccatggc gaggccggcc
      601 tcagaccaac gtcgagcttt atctgagacc gggcgagttg gattgctacc tccgctcccg
      661 aatcaacttc gtcgacctgc gcaacggcat ctatgccttc gagccgagcg acacactgga
      721 agaaaccctg ctgtcgcagg ccatcaatga gattcaccgg cggggcatgc tcgtctatgg
      781 cacggtcaac accggtgtgc ccgaaacaga gtatgacaaa gccatcgcga tgttcgaacg
      841 cttcatcgcc ttgggcgccg acggcctgtg ggtgtcgttc gatgacaagg gaccgggtga
      901 caaccccgag gaaatcgtcc gtcgcgtgct cgaactcggt cgcaaacacg ggataaccgg
      961 cgagcgaatc gcaatcaccc cgcccaaagg ggcctaccag gacattgccg cccccttcaa
     1021 ccggaagatc atggccgtcc ccgggatgga gtcggccctg tggttctgga cgcgatatcc
     1081 ctgtgattcg gatgtcgaag ccgcccgttc catcggattg aaagtcctgc cgagctggtg
     1141 gcacaactac ccgcggacgc tgaacagcca cgattacacc ggcattccgc cgctggccat
     1201 gggatggcac ggacccggct acgagctgct ggccgacgcc ggccgccagt tcacggccgt
     1261 gatgccctgg ggcggaaacg cgtggggccg gtattacgtg gtgccggtca tcggctggtg
     1321 gggctggaac cccaaggcgc atgactggca ggcaacacgc acgcggatcc atggtttcgt
     1381 gtttggcgaa agccagacgg ccgccgcggc cgagtttgac gagacgctgg cccggatacg
     1441 gcccttgctt cactacgccg tcggagacgg cgaatggcaa ccggccggcc cgccaaggtt
     1501 gaagaatccg gcggatcggc ggcgagggct ggcccttgtc gatcagatgg aggcattgct
     1561 gacgaagatc aaacacgccg caccggtcca gagcatgctc gatcccgacc agttgcaggc
     1621 cggttacctg agcaagatgg agaaggaggt cgccacgttg agagcagccg caacactgcc
     1681 tttcccggag tattggtgga tcgctcatca gcgggcggtg ctcgatgccg tgtacgacga
     1741 cgacatggcg cgagtcgagc ggttgaccgc cgccgtgcgc gagcctttgc tgcgagacat
     1801 cgacgaaatc aaccaaaagg gtcggcaact ggacaagatc gacgattacg tcaaatggtg
     1861 gacggagcag gccaacctgg acgccgccgg ctggaaaaac ctggtcgaga aacgccgcaa
     1921 ggaactggcc aatcgcttgg cggactacaa ttactttgtg aaggtccggg ccgacatgct
     1981 gaaagggttg gccgacccgc ccgtcgactg gggcacaggc cgctgggaat acgcaaatct
     2041 ggtacgagcc acagcgacgt tcacccaacg agagtactac tggggcaact ggatcgcggg
     2101 cttgtgcgat cacgaaggcg gcaaagttgc cgcgttcgcc accagccgcc gcacaccggc
     2161 cgcggacggc gagttcgcgg aactggagct ggctctgccg atttcgggtg atcgcagccg
     2221 actggccctg ctcatctacc tcgcggatgt gaacaaggaa ctgatcggcc tgcactacgt
     2281 gaggtggcga tgggccggtt accgcaggat tcaactcttg catcgcgacc acgtgctgtg
     2341 ggaagcggat atcggcgtgc agcgggaaca gggcgaatgg ttcatggtca agctgccgcc
     2401 ggtacctgag actgtgaagc aagtaacgct gcgcttgcgg gtcgagaacc tgcggggtgc
     2461 gatcggaaat cagacgcttg ccctggtcgg cccgattcgt ctgatcgagt tgccgatgcg
     2521 gaccaacgtg gactgagaac ggagagcacg tccgatgaat atgcgttttg cctgcctggt
     2581 catcccgctc gtttcctgga gcgcagccct tgccgcgaga atcgccccgc cgatccgggc
     2641 gcaaacaact cccatgccgg cagccatcca ggccggtcag gccgttgaca tcgtgctgga
     2701 tcgctactgg cgcggcaacg ttaaggttga tcaggacggg ctgcacgaca acaagaaaac
     2761 cttcccacat gtaccccacc tcatcgagta tcgctttgaa ttgccggtcg gcggcgaata
     2821 tgagcttcag ggtcgatacg tggccgagac cgacgctccc acgtacctga ccatcgacgg
     2881 caagctacag gggatgcgct tcgaggaagc cggcgatcat cgatcaaaat gggtttcgct
     2941 cgcgaagaca ccgttgacgc ccggcaaaca ctggatccgg tttacctcgc aatatgtgga
     3001 aacacccttt ccgacgctga ccgggctgcg tctggtgttc cacggcggac caggccccga
     3061 gccggagccc gagcccccca ttgtgggtcc caggccgtct ttgcccgacg actggtcgaa
     3121 aacgatcagc cgcaagatcc acagcgactt tcacactgcc ggcttcatcc gcggaatcgg
     3181 ctccagattc gacggcacaa ccttcggcaa gacgcttaga gacaacggcg tcaacgccat
     3241 ctgcatcttc gccaagggac atcacgggta cgcttactac gacacgaaag tcgggacgcg
     3301 acatccgggg ctggatttcg atctgatgaa agcccagatc gaggcttgcc gcaggtacgg
     3361 catcgccgtg tggacctatt tctccatcgc tccggatgaa ctctacacca gcacctgcga
     3421 ccagacgatc accgacccga acgaccggcc caccgacatg gaggtggacg tcgaatcgcc
     3481 ctacgtcgcc gattatctct ggccgatgat cgccgaatgc gtccgcaact atgacctcga
     3541 cgggctcttc ttcgactttc ccggaaacga ggaattcgtg cgagagaccg tgcgcctggt
     3601 caaacgcatc aagcccggca tggtcgtcgc ctataaccac cagtgggcca agagccgcga
     3661 tgaactcggc gagttggacg ttctcgaact ggagagctgg cgccacaagc agccgctcta
     3721 tcactggcag tattatgccc gctacgcccg tggcgcagtg cccctgacgg ccatgaccat
     3781 tcgtttccac aagagctggg gcgacttcgg cggaatcacc agcgaggcaa tgcttcgcgt
     3841 ccacgccgcc accgccatgg ccaatggctg cctgctcacc atcggcgatc atctgcaccc
     3901 ctccgggcaa ctcgacccgg cggtctacga gcgcatcggc cgtgttctgc gcgacgttat
     3961 gagaatcgag ccttacgtca ccggttccga gagcctccct tacgtcgccc tgatgcggcc
     4021 gaaagagatt gcactgatgg gtgcggacaa tccctgccac gctctgctcg attcaggcat
     4081 ccacttcacc gtcatcgacc cgtcgcaaga cctcaagccg tttacggccg tcctcatccc
     4141 cgaggcgaaa atggccgacg acgcgctgac cgcgcggttg aaggaatacg tcgagaacgg
     4201 cggcagactg ctcgctttcg gcaagccgcc ggcgagaatg gccgagctgt tgggcattga
     4261 ggcacagccc gcgaacgagg ccgcctacat ccggatcgat ccgcggatcc tcccaacccc
     4321 gcccgctacg gttctgtata cctacttgga agtcgctccg gcccgaccgc tggaggatac
     4381 aacgaccctc gccccgctcg tgtgggcgat gaatcacgga accatccata cttcgcgacg
     4441 ccagagccca ccctcggatg agccttccgg gctggcagcc atcaccagtc gcaagctcgc
     4501 caagggccaa gccgtctatt gcgccgccct gctgccggag gtctacgccc gatggggtta
     4561 ctcggccgta cgggagatcg tcgccgatct gctgaggtac atgattcctc ccgataagcg
     4621 cctggcagac gtcgaagcgc cggtccacct ggaagtctcg ctcaaccgcc aaggcaaccg
     4681 cgtcatcgtg catctggtcc acagcccgca aagccgcgcc agcctcggaa cgttcaacaa
     4741 ggatgacctg gtcaaccagg acccggtaat cgacgaaatg cccacggtcg cgggaacgag
     4801 gctgcgcctt gcagaaagcc ttgtcggtaa tcgcagcatc aagctgctcc cggcaggcac
     4861 ggaaatcagg cccgacagcc gtgcgaacgg tgtggtgaca atcagggtgc ccgatttcca
     4921 gatcagttca gtgctgctca tcgaatagaa ttcacgctga cgcgaggata tcgaaatgcg
     4981 ggttcatcgc ttggctatgg tcggatgtgg cgcgttcgcc aggctctatc acctcccgat
     5041 tattcacgcc aacccccgag cacaactggc ggttgtttgc gacctcaatc ccgatgtcgc
     5101 gaaggagtgt gccgaacgct ttcacgcccg caaagcgact gccgactggc acgaggtgat
     5161 cgacgatccc gacgtggacg cgatcattct cgccacgcat accaatctgc gcgccgaatt
     5221 gattatcccc gccctgcaag cgggcaaacc cgtcttcgtc gagaaacccg tcgccaacac
     5281 cgacgccgag atggttgaaa tcgtgcgggc cggccgcgcc gcccggctgc ccgtctgcgt
     5341 cgaccacaat cgccgctcaa gcccggccat gcacgatctg tgccaattgc ttcagaaggc
     5401 ccgcaacggc cccgggggcc atctcccctc ggttgaccgc agcgacggcg gcaaacgagc
     5461 ggccatgcca caggaaaagc agacacagat cctgatccgc gtcaatgacg actgccgcag
     5521 ttgggtcgac tgggcattaa ccgacgccga gggaatcctt tttgcggaaa tggtccactt
     5581 catcgacgtc gccatgtggc tcatgaaccc cctgccaatc acccgcgtct tcgccgaggg
     5641 ctcggcccag ggcaatttcg cactgatcat gcgattcgca gatggctcgc tggccaccat
     5701 acagcagacc atctgcggcc acttcgacta ccccaaggaa ctgatcgaag tcaccgccaa
     5761 ccacgtgacg atggccatgg agcaccacgt cgagttgcgt cagcgcggcc ttgaagatga
     5821 accgtttcgt cgcacatatc cgttcaagtc aatcgacggc ggtatcgaag acggcggcat
     5881 cgaggcgttt caccgcgcgg tgaccgagac cttcgataac gcccgccgca ccgggaaccc
     5941 gccggtgttc atctcccccg acaaaggcca cgccgcccac atcgaccgat tcctcgattg
     6001 catcgaaggc aaaggtgaga acccctgtga cgtcgtcgac gccgtgcggg tcacgcgagt
     6061 cgccctcaag ctgctcgaat cagtgcggag ggagcagccg gtggagatcc gtgatagcga
     6121 ctggcagatc gaggcgtagc atgacactcg atgccccgat tcaaagtaca actgctggcc
     6181 ccggaatccc ttccggtaca accgctggcc ccggaatccc ttccggggcc ttcaggttga
     6241 cgattcctac cgctggcccc ggaatccctt ccggggcctt caggttgacg attcctatcg
     6301 tcatcaacat tagtcccttc cgtcgcaact ggtgcgcatt ttgcgcgcat gttttggggc
     6361 ctcaagcggc gtggttgtgc aaggcctgcg gatagtgtcg gtcatggagc atgaggactg
     6421 cagtcatgag gacctgccct ttgtgagtga ggcgccagcg gcgagaacgc tggatcttgg
     6481 cgatgagtcc atgcaggtgg agacgcttga gcaaccggga gacgtaagcg gcctgacggc
     6541 gggattcgac cttgtttggg gtggtgaggc cgagatggtg gcggatgtct cggttggaga
     6601 agcccttgag caggtgctca ccgcgcatca ccgcggcgaa cagacggacg tcgtggcggc
     6661 aggccgcggg tgacgaacgt tccgaagtcc ttgatcaaaa tgccctgccg ggctatgaat
     6721 tgttccatgg cctcgggccg gcccaggagc agatggccct tgaacaggat ccggtcggag
     6781 caggaaatcg tgccagtgat ctgggcctga tgcttgtcga tgaatcgttc cacggcgttc
     6841 tcctttccat ggtttcataa accttaccac ggtcaggtaa gaacgccgtc tttgctatag
     6901 gggcttacca agccctcttt ggttgcggcc gaaggccgcc ttggattacg ctggtcccag
     6961 attcccatct gggaccactc ttctgctcgg cccggaattc cttccgggcc accctgctcg
     7021 cggaatccct tccgcattcg cgtttgaatg acgatcggaa tcgtcaaccg ggaggcccca
     7081 gaacggattc cggggccagc tatcagttgc gcgcccgaaa gataagtgtc tctccggctt
     7141 gcgtttcgag ctccagaagc tcgccgctca tcgtctccgt cgcatctccg cgaatcactt
     7201 cgaccttgcc cggccatggg ttgatgatcc gcagcggacc gccgacctcg gcgcggacac
     7261 ggatccatga cacccggccg ttcttcattt ccgcgctgac cagaaacgcc cccaccgccc
     7321 gaagatcctg gaacgccgcg tctttgtccc tcggccaatt cgggaagaac cgcagttgtc
     7381 ccgtatagct ttgcagaagg cattcgttga ccactaccgg cagcgagaaa ttctcaaacc
     7441 agatgcccat ccgcgccatg aaggcgtaat ccgtctcatc cgagtaccga ccgccgacct
     7501 gtagaaccat gtccgcgcac gtcccgttgg gcatcaggca atagctgatc tgccgtttga
     7561 acctctcgag atccagcacc cccagcctcg ccccctggag gttcaggaag accagctcgt
     7621 tgccgccttc gttgcgctgg ttgcgccacg tattcagtgc aatctcgagc acttccttcg
     7681 gcgaatgcag cccgatatcc tcaccgggga acaccggcat cagcggattg ggagtgttgt
     7741 agacctgctc ggggctctcg ccggagactg acacgaacac cttgcctcgc tggctctggg
     7801 cggtgatgta ttccggaaag tgatccagga tttcgcggac ctgccgggcc aattcggtct
     7861 cctcgcggtc gcgcccaagc accttgcacg cctccaggta agccttgaac acgaacttgg
     7921 tcagcgtcag gtccacgatg cagtcggcgg agaacgccgg atcgactttc agcccgtgca
     7981 gttctggcac caccgtcggg tagatatggt acttgtcatc attccactgc ggtccgtggg
     8041 cgtcaggccg tcgcatgtac gcattgagaa actgaactgc ctcgcaaatc ggtccgaagg
     8101 cccggtctcg cagaaactcc ttgtccatgg tgtacaggta gtgccaccac aggctctgca
     8161 caacccacga cgtctcacac acctccgccc cccaggtcgg caccggatac ggcatcatgg
     8221 tcatctcaac cgggtacgcc gagtgcggaa aaaacgcccc cggcagcttg tagtggttct
     8281 tcgcccattc gcggctgacc ggcaaaagaa aatccacgag gtccacgtat ggcaggtgtt
     8341 tctcaactcg gttggacgaa aacgtcaccc agaatggctg ctggagattg tagttggtgt
     8401 gataatcgcc gtgccaggcg gtgccgatgt tcccgtagct ccagttggca aacaggccgg
     8461 gacagacctc gcccggccgc accgcacaat tgagaaagta catgttgcgg taccaggtcc
     8521 gctcgagaaa ctcgtcgtcc agttgaacgc ccgaccggct ccagtaatcc ttccacgccc
     8581 cggcagcctc ggccgacgcg gcggcaaaag catcatcgct gggttcgggg acttcggcct
     8641 cgggcttgga aagaacgcgg gccatgccgt gttccaactg cacacatgcc acgaaggggc
     8701 cgtcctgcac cagcggcgcg ccgatccggc cgtttacccg cacggccacg cgcagggcct
     8761 tatccttgtc acgatcatca cccagcaccg gaagcacctg cctgaacgag agcgtgtccg
     8821 ccgtggatgc cgtttgcgtg gccatcccct tttcaggaat cagacgcacg cggtcaaacg
     8881 gcgcctggat cggccggccc tcggcatcca ccatccgcat ccaaagccga tccgcattca
     8941 tttcgttgaa gagaaccagt gtgaccgtcc ggccgtcgat caggaattcc acttcgcacc
     9001 tgccggtatc gattcggacg cgatgcccga gcagttccgc cttgcgccgg tcgaacccca
     9061 gcaacaccga tccgcacggc cagggccgcg gataaggctg ggcgtaattc tctcgggcca
     9121 tcttgcagta gtcatcgaac caagccttat cctccggcat gccgcgcttc agccgggccc
     9181 acaattcgtc gaacgtcgcc agcttctcgc gattgttctc ggctacgcgg atgtcccaaa
     9241 ccccgttgtg cccgagatgg atcatgaccg aatcaggacg cgtacagacg atcaccccca
     9301 tcccgccgtt gcccagaacc gccccctcga agaaatcctg ggccggcttg ttcatctcga
     9361 tggcatgcaa cccggcacgt tgttgaggat cgaacctcaa ctccgcggcc ggacgcccgc
     9421 aagcggcgag aagcgtaagc gagaacaagg cggcatgatt caggcatttg tgcaacagca
     9481 tggtgtacct cctgctgatg tacgacccag aacgcgtgca aaaccatcaa gaccacatgg
     9541 tagggcagcg tcgaggtcgc cgcagcgacc gagacctgcc gtatctg
//
LOCUS       DAOTPW010000224         9463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6660_length_9463_cov_4.630102, whole genome shotgun sequence.
ACCESSION   DAOTPW010000224 DAOTPW010000000
VERSION     DAOTPW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6660_length_9463_cov_4.630102"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..801)
                     /locus_tag="P5316_17190"
     CDS             complement(229..801)
                     /locus_tag="P5316_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRR86748.1"
                     /translation="MTLADCMRLLGLPETATVEEIKRAYRRLAQRLHPDKKRGDLDAQ
                     SQFIEVSKAYRMLVSAARACRQGKPVGTCRDCGDFGEVSIGLDGHPRCGRCVFHPAGG
                     RLLPLPVYVVVKCTVTIVLLAAGAYLLLLATATDSKAYGMAAACAGMLGLISLAITCL
                     SIVRCIHPGDRVRQRNWSSIRRRLRIDGPS"
     gene            complement(883..1563)
                     /locus_tag="P5316_17195"
     CDS             complement(883..1563)
                     /locus_tag="P5316_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86749.1"
                     /translation="MRDPSNNRVGQARQALSRVLVLGVGLATVTGCGPEFGAVIYHLG
                     VGAEQKVRAEFKLPAGPVLILVDDEMDLVSPPSTRETLVDELATRLKEHKVVERVTTN
                     EELALIRRSAADFDELSVREVGRRANADTVIWMNVEDFYVDRDLEMMVAPARFGVKLK
                     VFNAREEDRLKIRLWPPERDGRRIDITISPHEARRCKSRSELDRLLASTMADEVAKLF
                     YDHKVETR"
     gene            complement(1544..2194)
                     /locus_tag="P5316_17200"
     CDS             complement(1544..2194)
                     /locus_tag="P5316_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86750.1"
                     /translation="MEIKRHPARPALGILISASIAVLAGCGPTGAFLAATLPDTEKVP
                     PEFNRLPGRTVLIHVYALPEIRWAYEKVSLDVAAYLADHLARHVKGVNIVDVVRVSSY
                     MEKTPGSENDPVGVGKHFNAEMVIEVSVYKLSVRDPGMAHFYRGRMAASVVVYDLTRP
                     NEPPERIPLGDVEVAVPEEGPVGLTGVTAVQVRQQTYLAFATQVGRKFHEYERPIE"
     gene            complement(2223..2840)
                     /locus_tag="P5316_17205"
     CDS             complement(2223..2840)
                     /locus_tag="P5316_17205"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRR86751.1"
                     /translation="MRSPGDPAGLTGIGEMATDWDESQTAEDLIVPTGGAGSGVRRAR
                     PVANPLGPIIEIRHLERPRIGRLYAGGVLLAAGVIVGIGLWLVPAQGHVGTHRQLGLL
                     PCGFLTMTGYPCPTCGMTTAFAHAVRGQVVDSIRSQPAGFVLALVTILIGASAAGAMT
                     VGRYPAINWYRVNPTSFVWYAAVGLVLAWGVKIALGLLDGSLPAR"
     gene            complement(2956..3450)
                     /locus_tag="P5316_17210"
     CDS             complement(2956..3450)
                     /locus_tag="P5316_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86752.1"
                     /translation="MSSVDSEVLMPCAGCGASIYREHIHRGLAGMWAGQMLCPACLKP
                     KRESGAAEPTGEAGDSIPFPLAPVEGPEADASVEVASAPQPETAPGESASGEVSIEKG
                     VGARRVRTFHAKLSEAALRHLDEQVNTWLKDNPGVRVKFANTTVGVFEGKHSEPNLVL
                     TVFY"
     gene            complement(3708..3923)
                     /locus_tag="P5316_17215"
     CDS             complement(3708..3923)
                     /locus_tag="P5316_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86753.1"
                     /translation="MKVRRGGIRNRLAMEVLFLAGYWLFQGGCLGFVQRELEVLVAAE
                     SNGVLIRSSFLYEVFGPRLIALANQWL"
     gene            complement(3937..4233)
                     /locus_tag="P5316_17220"
     CDS             complement(3937..4233)
                     /locus_tag="P5316_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRR86754.1"
                     /translation="MDLRDFQRLIDKMYSHKDRERGSAGTFLWMMEEIGELAQAIGQG
                     SDQQTKAAEFADVMAWLVTLANVEGVDLSEALHAKYGQGCPGCGKMVCTCDAKP"
     assembly_gap    5694..5703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5704..8213)
                     /locus_tag="P5316_17225"
     CDS             complement(<5704..8213)
                     /locus_tag="P5316_17225"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRR86755.1"
                     /translation="MVAIVKDRQGENDTDPKSRPDLGLPENALRVLRARYLKKDESGQ
                     VVESPRDLFRRVAKTIADVETRYGADAAQRDAWEEAFFDMMVERRFLPNSPTLMNAGR
                     EMGMLSACFVLPVRDSISEIFDAIKQTALIQKAGGGTGFAFDELRPTGDFIKSSGGTT
                     SGPISFWRAFSEATNAIQQGAFRRGANMGMMNVHHPDILKFLNAKQDLTQFTNYNISV
                     KITDQWMEEFLEDEDSPHVVRNPRTGQEFVMPRDLKIWEYDVRSLIPVRRGPNGELRV
                     MHQSGETEEVVTRGSALSRPVYTKRDIWRIIVENAHRTGEPGVVFIDRINEYNPTPHL
                     GRIEATNPCGEQPLLPYEACNLGSINLAKFVVESEGHGRVDWDGLRATVHHATRFLDN
                     VIDANNYPLPEIEAMCKGNRKIGLGVMGFADALYKLGVPYNSEEGVAWGERFMKFIND
                     ESHNYSEQLAMERGCFPNWSGSVWDTRYHRPMRNAAVTTVAPTGTISIMADCSGGIEP
                     MFSLAFFRNVLKGQEEGAKPMIQVNSIFERVARERGFLSEGLMDRIAREGTLVELDEI
                     PEDVKRVFVCAHDISPEWHVRMQAAFQRHCDASISKTINFPRDATVEEVDAIYRRAYE
                     LRCKGITVYRDGCRDAQPMALKDKEQSANPSVPNASASSRSAPGEFKELEPRDIPEII
                     SGLRLRQMTPFGNMHVKITVDPKTERELEVFAQLGKGGDIATSDLEAICRMTSLWLRS
                     GGSLRHVIRQLKDIGSSLQVPTREGKIMSLGDGLARAMQKYMRAKERFGLRNLLLGEY
                     DLAELDRPASALPGKTNGNGNGKSKGHELVSEVRHATAA"
     gene            8801..>9463
                     /locus_tag="P5316_17230"
     CDS             8801..>9463
                     /locus_tag="P5316_17230"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRR86756.1"
                     /translation="MTANETVFRAMSDATRQKLLRVLSDYDLSVSELVEILRMPQSTV
                     SRHLKVLRDAGLLVDRRVGTTVMYAVRPPSDAAVVPGRPPGSQGSLRPMSNGVIGLRD
                     RLLEWVGQAQLDGDVADRLQKVVRRRHAGDRGFFDALAAKWDRLRIDAFGEAFHLEAL
                     TALLPTEWTAADIGSGTGYLLPVLSARFRKVIAVDPAPRMIEIARNRPEVRAADNIEF
                     REG"
BASE COUNT         1960 a         3146 c         2513 g         1834 t
ORIGIN      
        1 tccaggttgc cgccttcact cgactctccg ccatccgcta tccgcctgtt ccgtcggaca
       61 tcaaggtcgg cccacagcat agccgaaccc caaagcccag gccagcggaa cggcgttcag
      121 gtcaacgccg cgcccaaggc aatgaaatgg atcccaaaga gaaaacgcca gggcaggcag
      181 gcaacgccgt tggaagtatc cggcggctcg tccgtcctca cctcttcctc acgacggtcc
      241 gtcgattcgc aatcgcctcc ggatactcga ccaattcctt tgcctgaccc gatcccccgg
      301 atgaatgcac cgcacgatgc tcaggcaggt tatcgccaac gagatcagtc ccagcatgcc
      361 ggcgcacgcc gcagccatgc cgtatgcctt actgtcggtc gccgtcgcca acagcagcag
      421 gtacgcaccc gctgctaaca acacgatggt caccgtgcat ttgacaacca cgtacaccgg
      481 cagcggaagc agccggccgc cggccgggtg gaaaacacac cttccgcaac gcggatggcc
      541 gtcaagcccg atgctcacct cgccgaaatc accgcagtca cggcacgtgc caaccggctt
      601 gccctgtcga caagcccgtg ccgcactcac cagcatacga taggcctttg agacttcaat
      661 aaactgcgac tgggcgtcaa gatcgcccct tttcttgtcg ggatgcagtc gttgtgccag
      721 gcgtcggtac gcccgcttga tttcctcgac ggtcgcggtc tctggcagtc ccaacagtcg
      781 catgcaatcg gcgagagtca tccgctgtgc gttcgtccga taccttcagg aaagtggctg
      841 atcccacgac ccacaccggc aggattcaca tgtggcaacg gatcaccgcg tttcaacctt
      901 gtgatcatag aacagcttgg ccacctcgtc ggccatcgtg ctcgccagga gccggtcgag
      961 ttcggaacgg ctcttgcatc tgcgggcctc atgagggctg atggtgatgt caatccgccg
     1021 gccatctcgc tccggaggcc agaggcggat cttgagccgg tcctcctccc tggcattgaa
     1081 aaccttcagc ttcaccccga accgggccgg agcgaccatc atttccaggt ctctgtcaac
     1141 gtaaaagtcc tcgacgttca tccagataac cgtgtcggcg ttcgctcggc gaccgacctc
     1201 gcggactgaa agctcgtcaa aatccgccgc cgaccggcga atcaaggcga gctcttcatt
     1261 ggtcgtcacc cgttccacaa ccttgtgttc cttgagccgc gtcgccagct cgtcaaccag
     1321 tgtctcacgc gtcgagggcg gagatacgag atccatttca tcatcgacaa gaataagcac
     1381 cgggccggcc ggaagcttga actccgcacg aaccttctgc tccgccccca ccccaaggtg
     1441 gtagatcacg gcaccaaact ccggcccgca accggtgacg gtcgccaagc cgacacccag
     1501 cacgaggacg cgagacaacg cctgtctcgc ttgcccgacc cgattattcg atgggtctct
     1561 catattcatg gaacttcctg cccacctggg tcgcgaatgc gagataggtt tgctgacgca
     1621 cttgaactgc ggtgacaccc gtcagaccga caggcccttc ctcgggaacc gccacctcta
     1681 cgtcgcccag aggaatccgc tccggcggct cgttgggtcg tgtcagatcg tagaccacga
     1741 ccgatgctgc catgcgcccg cgataaaagt gggccatgcc cggatcacgg acgctgagct
     1801 tgtagaccga cacctcgatg accatctcgg cattgaaatg cttcccgacg cccaccgggt
     1861 cgttctcgct tcccggcgtc ttttccatgt agctgctgac ccgcaccacg tccacgatgt
     1921 tcacgccctt cacgtgccgc gcaaggtggt ctgccaggta cgcggccacg tccagcgaca
     1981 ccttttcgta cgcccagcgg atttcaggaa gggcgtagac gtggatcagg acggttcggc
     2041 ctggcagtcg attgaactcg ggcggcacct tctcggtgtc cggaagtgtc gcggccagaa
     2101 aggctcccgt cggcccgcat ccagcgagca ccgcgatact tgcggaaatc agaattccca
     2161 gggccggtct tgccggatgc cgcttgatct ccatgtcaat cctccaggac gcgatgtgca
     2221 ggctaccggg cgggaagact gccgtccagc agtcccaggg caatcttgac tccccacgcc
     2281 agcaccagcc ccacagccgc ataccacacg aaactcgtcg ggttgacccg ataccagttg
     2341 atcgccgggt aacgcccgac ggtcatggcc ccggcggcgc tggccccgat caggatcgtc
     2401 acaagagcaa gcacaaatcc cgccggttgc gaccggatcg agtcgaccac ctgcccgcga
     2461 acagcatggg caaacgccgt tgtcataccg caggtcggac acggatagcc ggtcatggtg
     2521 agaaaaccac agggcaagag acccagttgt cggtgggtgc ccacatgacc ctgggcagga
     2581 accagccaaa gaccgatgcc gacaatgaca ccggctgcca gcaaaacgcc cccggcgtaa
     2641 agccggccga ttcgcggcct ctcaagatgc cggatctcga tgatcggccc aagtggattg
     2701 gccacaggtc gggcgcgccg aacgccactg cctgcccccc ccgtcggaac aatcaggtct
     2761 tccgccgtct gcgactcgtc ccaatcagtt gccatctcgc cgattcccgt cagcccggcg
     2821 gggtcgccgg gtgacctcat ggaaggcaat ctcggttggc catcaactct tgcgcctctc
     2881 aacattatca ttctgcggtg gcgaggtcaa cgtggtgcgg cgaacggctc aggattcctc
     2941 tcgctgttga aacaatcagt agaaaaccgt caaaacgagg ttgggctcgc tgtgcttacc
     3001 ctcaaaaacg ccgacggtcg tgttcgcgaa cttgaccctg accccgggat tgtccttgag
     3061 ccaagtattg acttgctcat ccaagtgccg cagagctgcc tcggacagct tggcatgaaa
     3121 ggtccgcacg cgccgcgcac ccacaccctt ttcgatcgac acttccccgg atgcggactc
     3181 accgggcgcc gtctccggtt gcggggccga agcaacctcg acagaggcgt cggcctcggg
     3241 cccctcgacc ggagccaaag gaaatggaat cgagtccccg gcctcaccag tcggctcggc
     3301 tgcaccggat tctcgctttg gcttgaggca agccgggcac aacatctgcc ccgcccacat
     3361 acccgctagt ccccgatgaa tgtgctcacg gtagatgctg gcgccacagc cggcacacgg
     3421 catgagaacc tcggagtcga cactactcat gatcacctcc cattcccaag ctgtcagatg
     3481 tgcctgtcga cggtgagccc caagacctga tcggacagag atgccaaacc ggccggactc
     3541 gagcctcaca cgaccctgag atgcattcta cctcggcccg tggaaaggaa tcaatgcctg
     3601 caagacacaa gagacgcctt tgcgtcaatc acgcgccgag atgcacagag gctttgccgc
     3661 cggggcgcag cgacagcctt gcggatcccc tcaggaaggc agatgcctca aagccactgg
     3721 tttgccaggg caatcagccg aggcccgaag acctcataga gaaaacttga acgaatcaag
     3781 acgccgttgg actcagccgc aaccaggact tcgagttcgc gctggacgaa gcccagacat
     3841 cctccctgga acagccagta acccgccaag aacaacacct ccatggcaag tcggttccgg
     3901 attccgccgc gtcgcacttt catgttgatc cctctgctac ggtttcgcat cgcaggtgca
     3961 taccatcttg ccgcaaccag gacaaccttg cccatacttg gcatgaaggg cctcgctcag
     4021 gtcaacaccc tcaacgttgg ccaaggtcac cagccaggcc atgacatcgg caaactcggc
     4081 ggccttggtc tgctggtccg agccctgccc gatggcctga gccagttccc cgatttcttc
     4141 catcatccat aggaacgtcc cggcgctgcc ccgctcacgg tccttgtgcg agtacatctt
     4201 gtcaatgagt cgctgaaaat cgcgaagatc catctaggcc tcccaggctc ttgttcgggc
     4261 ctctccctcc atgggcgagc gtcacctacg atacttggtg cccagttaag cggcaatcac
     4321 gtgttcgggt atataatcac ggccatggcc gatccctcac gtccaagacg ataccgcttg
     4381 ccaagatccg ctcgagtcaa atcgcaacag gtctttcgac gggcgttctc tgagcgatgc
     4441 cgtgcctcgg accaacatat ggttgtgttc gcggtgccta acgattccgg cttcgctcga
     4501 ctcggcatct ccgtaggcaa agcctacggc cgggcggtac aacgaaatcg aataaaacgc
     4561 ctaatccggg aagccttccg gcacgtcaga cacgagctcc cagcaggaat cgacttgatc
     4621 atcgtacccc gaaaagagaa cattgagcca accatctttg atctccaagt gtcgatccgt
     4681 cgcctctcgc gcaaactgca gcgtcgaacg cagtgaacgg cagacggtta cctctcaaga
     4741 cgaacatccg atccgacgag aactcgcctc actaatccag gaacaatcca gaagactcga
     4801 aaaaaaaaga aagcaccagg tgcccaaagg gcgggcccgg tgctttccgg aggggaaaga
     4861 agccattgct caatttgagc tcttgtcggt atgccctcct atgactccca atcaggctgg
     4921 ctccatatga ttataggcca ccccattagt gctttatcgg ccagacaagg ccacaatttt
     4981 agcctttcct tcttcaggcg cggcagggcg cacctttcac ccgacaacat ggggctttta
     5041 ccccatagcc gccagccaaa tcgcggctta cgtccgaata actcggcctt gctaccgctt
     5101 cctgcccctt ataccccggc tcaccgggga gcgtcgcacc cgtagcaccc cggctgaaca
     5161 ggagcttcta ccacaaacac gttcttctgt caaaccctgc ttggccccct cgcgcgccct
     5221 atcgcggacc gccttcttgc gggccgcaac ccaaacgcac cgcgatcccc aagtcacgcg
     5281 cgcaagccgt acagatgtct agctccaaag cggccttcgg ccgcaaccaa gaaaggccgc
     5341 tcgtttccga tcgtaagctg ttgttcgcaa gcgatttacg cgccgcaacc caaaaacgcg
     5401 cgcaaaactc actccagtcg cgacggaaga gactaaggcg gaccgccttt ttgtgggccg
     5461 caacccaagt gttccgcgat ccagaaacat gcgcgcaaac cgcacagata tcccgccgca
     5521 aggctctaac actgcgccca gccgcaggac gggcacttgt tacacccttc cgagaaaacc
     5581 aatgcgtccc cgcactccgg gcatttgacc ttgaatgcca gccggtctcg ctcaatccgg
     5641 gatacgccca cgctcccggc caacgtgggc gaccccagaa ctggagcccc atgnnnnnnn
     5701 nnngctgccg tagcgtggcg cacttccgag accagctcat ggccctttga cttaccgttc
     5761 ccgttgccgt ttgtcttgcc gggcagcgca ctcgcgggac ggtccagctc cgccaggtca
     5821 tactcaccca gaagcagatt ccgaagacca aaccgttcct tcgcccgcat gtatttctgc
     5881 atggcgcggg ctaagccgtc tccaaggctc atgatcttgc cctcgcgggt cggcacctgg
     5941 agactcgaac caatgtcttt gagctgtcgg atcacatgtc tcagggaacc cccggaacgg
     6001 agccacagcg acgtcatccg acagatcgcc tcgagatcac tagtggcgat gtccccgccc
     6061 ttgcccagtt gggcaaatac ctccaactca cgttccgtct tcgggtcgac cgtgatcttg
     6121 acgtgcatgt tgccgaacgg agtcatctgt cgaagccgca aaccgctgat gatctccgga
     6181 atgtcccgag gctcgagctc cttgaactct cccggcgcag aacgcgagct agcactcgca
     6241 ttcgggaccg aagggtttgc ggattgttcc ttgtctttca gagccatcgg ctgggcatcg
     6301 cggcaaccat cccgatacac cgtaatgccc ttgcatcgca actcgtacgc acgtcggtag
     6361 atggcgtcca cctcctccac ggtcgcgtcc ctcgggaagt tgatcgtctt ggaaatggac
     6421 gcgtcgcagt ggcgctggaa agccgcctgc atgcgtacgt gccattccgg cgaaatatcg
     6481 tgcgcgcaga cgaagactcg tttcacgtcc tccgggattt cgtcaagttc aacgagggtg
     6541 ccttccctgg caatccgatc catgagaccc tcgctgagaa atcctcgctc tctcgctacg
     6601 cgctcgaaaa tcgagttaac ctgtatcatc ggtttcgcgc cctcttcctg tcccttcagg
     6661 acgttgcgga agaaggcaag actgaacatg ggttcgattc cacccgaaca gtccgccatg
     6721 atgctgatcg taccggtcgg ggccaccgtc gttaccgccg cgttgcgcat cggccggtga
     6781 taccgcgtgt cccaaacgct tccggaccag ttggggaaac aaccacgctc catggccagt
     6841 tgttcgctgt aattgtggga ttcgtcattg atgaatttca tgaaccgctc gccccaggcc
     6901 acgccttcct cggagttgta cggcactccg agcttgtaaa gcgcgtcggc gaaacccatc
     6961 acgcccaagc ctatcttgcg gttgcccttg cacatcgcct cgatctcggg caacggatag
     7021 ttgttcgcgt caatcacgtt gtccagaaac cgcgtcgcgt gatggacggt ggcccgtaac
     7081 ccatcccaat ccacccggcc gtggccttcg ctttcgacga cgaacttggc caggttgatg
     7141 ctgccaagat tgcaggcctc gtagggcagt agcggctgct cgccgcaggg gttggtcgcc
     7201 tcgatacggc ctaggtgcgg cgtcgggttg tattcgttga tccgatcgat gaaaacgacc
     7261 cccggttcgc ccgtccggtg agcattctcg acgatgattc gccagatatc ccgcttcgtg
     7321 tacaccggcc tgctcaaagc cgagccgcga gtgacgacct cctcggtttc acccgattgg
     7381 tgcatcactc gcaactcgcc gttgggaccg cgcctcacag gaatcaaaga ccgcacatcg
     7441 tactcccaga tcttcaggtc tcgcggcatc acgaattcct gaccggtccg ggggttgcga
     7501 acgacgtgcg gagaatcctc gtcctccaga aactcctcca tccactgatc ggtgatcttg
     7561 accgagatgt tgtaattggt gaactgcgtc agatcctgct tggcattcag aaacttcaaa
     7621 atgtcagggt gatgaacgtt catcatcccc atgttcgctc cacggcgaaa cgcgccctgt
     7681 tggatcgcgt tggtcgcctc gctgaacgct cgccagaagc tgatcggccc cgatgtcgtc
     7741 cccccggagc tcttgatgaa gtcacccgtc ggacgaagct cgtcaaaagc aaagcccgta
     7801 ccgccccccg ctttctggat cagcgccgtc tgcttgatgg cgtcgaatat ctcactgatg
     7861 ctgtcccgaa cgggcagcac gaagcaggcg ctcagcatgc ccatctcacg cccggcgttc
     7921 atcagcgtgg gactattcgg cagaaaccgt cgctcgacca tcatgtcgaa gaacgcttct
     7981 tcccaggcat cgcgctgtgc cgcatccgcc ccatagcggg tttccacatc cgcaatggtc
     8041 ttggccacgc gacggaacaa gtccctcggc gattccacca cttggccgga ctcgtccttc
     8101 ttgagataac gagcacgcag cacgcggagg gcattctcgg gcagacccag atcgggcctg
     8161 ctcttgggat ctgtgtcgtt ctcgccttgt cgatccttga caatagcgac cattgaccaa
     8221 ctccttcagg ccgcaacggc ccccgtttca acatcaaagc agaccaaaaa cacgactcgt
     8281 gctccttgcc tccccacgcg cagaccacac cctcggaagc acgccttcgc cttccgcttt
     8341 ccctgggctt tagggtttga ttaggtctcc aagaacgacc tgtgccccta catcttgggg
     8401 gcagtattct taataactca atatctgccc aaagtcaacg gcattaccgc ccctagattc
     8461 aggaaattat aacactcact tacaagtccc tgtcaaaggg gaagttaaac gcccaacgaa
     8521 tagttattaa caagctattt ttctatatgt cggcggtcca gccgacctgg cccctatatt
     8581 ttgtggcacg ggcaaaccaa agtcaagccg ataatcggga aaaaagcggt gttccgggtt
     8641 cctgcgagtc ttggccaaat cccaccaagg aataccgtct cagggcagaa agccctcatt
     8701 ctggctgcca gccatgtccg cctgccttgg ccctcccaaa aactcgcttg cttcctgtca
     8761 tctaccgata tatcctatca tccggatacc tggattggcc atgacggcaa acgaaaccgt
     8821 attcagagcg atgtcagatg cgacccgcca gaagctcctt cgggtactgt cggactacga
     8881 tctgagcgtg tccgagttgg tcgagatcct gcggatgccg caatccacgg tcagccggca
     8941 tttgaaggtg cttcgcgatg ccggtctgct ggtggatcgg cgagtgggaa caacggtcat
     9001 gtacgccgtc cggcccccaa gcgacgctgc cgtcgttccg gggaggccgc caggttcgca
     9061 gggatcactc aggccgatga gcaacggtgt catcggcctg cgagatcggt tgctcgaatg
     9121 ggtgggacaa gcccaactcg acggcgacgt cgccgatcgg ctacagaaag tcgtccgccg
     9181 ccgacacgcc ggagaccgag gtttctttga cgccctcgcg gccaagtggg atcggttgcg
     9241 gatcgacgct ttcggagagg cctttcacct tgaggccctg accgcccttc tgccgacgga
     9301 gtggaccgcc gcagatatcg gcagcggcac cggctatctc ctgcccgtgc tatccgcacg
     9361 attccgcaag gtcatcgcag tggatccggc tccccgaatg attgagatcg cccgaaaccg
     9421 accggaggtc cgcgcagcag ataatatcga atttcgcgaa ggg
//
LOCUS       DAOTPW010000225         9455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6666_length_9455_cov_4.345000, whole genome shotgun sequence.
ACCESSION   DAOTPW010000225 DAOTPW010000000
VERSION     DAOTPW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6666_length_9455_cov_4.345000"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="P5316_17235"
     CDS             <1..997
                     /locus_tag="P5316_17235"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR86757.1"
                     /translation="ETATEEQLENLRLLVFYDNAAEPAVDASVACFFGAGRSRAPYRS
                     IPLGTDSPDGFYCYWPMPFRRSVSVVLSNTGADAVPVDSAVVEYEPGSFDPALGYLHA
                     KTSNTIRQSGQVFHPILSADGCGHYVGNLLYIHESRNAFSMLEGDEVIAVDGVTTCYG
                     TGTEDAYNGGYYYNWVAVQSDEPEGTYPPSATRPLHGIIYVRRDATPIARADQYRWYI
                     ADRVPFCQSIRVDIECRYALVGAEYTSVAFWYQLPPVPGNFDNDCDVDADDFARFESC
                     ATGPGVPGSASPACTMVQFEAADFDHDGDVDQRDFGIFQLCYSGENVLADPNCGS"
     gene            1142..2179
                     /locus_tag="P5316_17240"
     CDS             1142..2179
                     /locus_tag="P5316_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRR86758.1"
                     /translation="MSTVMFRRCPENPIVRPGLYPWRRAVTFNPAALYENGKVYLYER
                     AAGGLRPFHCYIGLLESGDGVHFRHVSDQPVFTPEMAGNRYGTVQDPRIVKLDGLYYL
                     SFAFRPYAWNIWPTGLGVPEAAQPTFPGFSGRDEDNQTRSGIAVSTDRLHWKLHSWVS
                     TPDIDDRNVILFPEKLAGRYAVLRRPSPFAGVTTEHRSVHPGIKISYSDDLHNWSTPA
                     TVIEPAYQWENNRIGGSTPPIRTPAGWLAFYHGVENQDPTVRRVCYRMGAMLLDADDP
                     TKVLARTPKPLLEPTEYYECFGLFIPNVVFPTGAVVIDDVVHLYYGACDTAIALATCP
                     LSELLDYMLSL"
     gene            2450..4048
                     /locus_tag="P5316_17245"
     CDS             2450..4048
                     /locus_tag="P5316_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRR86759.1"
                     /translation="MLIIGLDGATFDVLKPMMKHGRMPNLARLVKEGTSGILYSTQPP
                     ITPAAWTTFMTGKGPGRHGIVDFERYDVATGKLSFNSTYEIRERTIWEILSEKGFRVG
                     AVHVPMTYPPRKVNGFMISGFETPSVAAEFTWPPELKQEILRRWPNYSYRADWQRRVF
                     GRDKALRRNLRHIERSFHQGAELTTFCGERFGWDVLMVLFKLVDNLQHKTWKYLDPKT
                     AVRYPHEAELAANCFVELDKALGMLFDYARRYSASILMMSDHGHGSLDGKAQPNLLLK
                     RWGYLKIRSSTVQIRRKLSKIADRFLGRRRTRFDANLGIEHELVVDWEQTRACVIHAG
                     MNGFLYISLKGRQPHGVVEPSDYEKVRDDLRNRFLAAKCTDPAGREVRIFSEVHKPEE
                     LYGCNREEQPWMPDLLLVPQPGLAVVRRIVGRQPVRWSSLRRLEGTHRVEGILIANGP
                     HIRQGSQTTGRIVDIAPTLLAALGLKVPSDMEGNVLTDLFDRMPTIEYELPQTAEWTR
                     QECVYSEAEKEILTKRLTELGYLE"
     gene            complement(4085..6082)
                     /locus_tag="P5316_17250"
     CDS             complement(4085..6082)
                     /locus_tag="P5316_17250"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRR86760.1"
                     /translation="MAKRGEEMRTFTILWIAAGLCLLSSGGLQAQPQSLPDDGALRGR
                     AEKARLLATQALEAKKAAWASAQRHGWSPRTQHGQVVSELMGIRDGRVYICQTANSDA
                     GITAAADLVRNTPPYDINGFGVVVGVWDGGAVRATHRELTGRVSIRDSGSSFDHATHV
                     AGTIGATGVDPSAIGMAPSAGIDSYDWTSDLGEMTARAMAVPGQPDAIQISNHSYVHA
                     AGWDNSSGSFRWHGTWGERESSYFGQYDEVARQWDELCYEAPYYLPFKAAGNERNDPV
                     PAAGALFEYWFNGRWRQKSYDPNTDPLPDNWDAGGYDTLASASTAKNVMVVGAVSDAV
                     YGGKRLLSLATVTPFTSWGPTDDGRIKPDLVSSGMTLRSCTATGDASYGTYSGTSMAT
                     AVVSGSAALLVEYYGRLFPGQYMRSEMLRGLMIHTADDLQAAGPDYQTGWGLLNVEAA
                     AEQLLLHRRSPAARRIVSGLLSPAQPSISYTCASNGVRPLKVTLCWTDPPGVVRTGLD
                     NPSPCLVNDLDLRVVAPDGGTVHMPYVLDPLFPTSPATTGDNTRDNVEQILVSCPVAG
                     SYTVHVSHKGNLVGVLQYYALLVSGISAVITGDYDRDEDVDAFDMAEWEDCASAPGVP
                     AMAGCDGKDLDEDGDVDQCDFAVLQRCYSGLGGVPPPDCFE"
     gene            complement(6675..>9455)
                     /locus_tag="P5316_17255"
     CDS             complement(6675..>9455)
                     /locus_tag="P5316_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR86761.1"
                     /translation="GITVTGIGPIKTGAVWYRALTRYLTTISAFQDAYHAFNQAAQDL
                     IGTKPADPRTGLPSDSTFTVADAQQVDNALRAVQMDMPGRCGDFFSREEPVLCGGRVT
                     LYSESFESTDGGWSRGNSEWQWKSGMPGRSGKGWYCPDPSNPSGAAKQTLTSPEITIP
                     PGVMGLGVVFAHYFDVEEWWDGGNISMRINSGAWTLVTSFIHNSYTSSLARADYSSND
                     NPMAGQIVWTGSSGGWGKSAFTLPGSVKAGDKVQFRFDFGRDSFYGTNVGWYLDDFEV
                     FACHSGPSFELDGRPVIDDSAGGDGDGSVEPGETVDLTITLKNVGGDATNVSATLSCD
                     VGTVTVVGANATQSYPDLPTGGVGTNAGPFKIQVKAQHPMCEAIPLLLSVTAAEGDDE
                     IEFVVQVNGLPDPDGDGIGEVCDNCPTVPNPDQRNRDSDSLGDACDNCPVVANEDQAD
                     ADGDAAGDACDNCLLVPNPDQTDSDRDGLGNACDNCPLTLNPDQHDDDGDGVGDACDN
                     CPLIANPNQADDDKDGIGDACDNCVSAVNPFQQDADGDAVGDACDNCVKVANPDQTNS
                     DSDKLGDACDNCPEIPNPAQRDKDEDGIGDVCDTCTDTDGDGTGNPGFAANTCPQDNC
                     PNIANADQANADDDLWGDACDTCTDSDGDRFGDPGFPNNTCPIDNCPNDPNKQDPGLC
                     GCGLLEKDVDFDGKVDCVDECPNDPYKSVPGKCGCGVLDRDRDGDGTPDCHDTCDVDP
                     KKVQPGVCGCGVPDADDDKDGKLNCLDNCPTVANANQKDSDKDGVGDACDKCPNIYDP
                     TQDDADDDGIGDMCDNCVGVPNADQADSDGDGVGDACDNCPRVANADQLDQDGDGIGD
                     ACDASVSSAGKKDQIMPADNQTAVPTNEEPALDAPSSGRAVPCGAGAAQLMAVGLVGL
                     CVLRLVVRRRGC"
BASE COUNT         1826 a         3182 c         2675 g         1772 t
ORIGIN      
        1 ggaaacggcg accgaagagc aactggagaa ccttcgtctg ctcgtattct atgacaacgc
       61 cgctgagccg gccgtcgacg cctccgtcgc ctgcttcttc ggtgccggca gatcgcgggc
      121 accctaccgc agcattccac tgggtaccga ttcgcctgac ggcttctatt gctactggcc
      181 catgccgttc cgccgctccg tgtcagttgt gttgtcaaat accggcgcgg atgccgtccc
      241 ggtggactcc gccgtcgtcg agtacgagcc tggctcgttc gatccggccc tggggtactt
      301 gcacgccaag accagcaata ccatccgcca gtccgggcag gtcttccacc ccatcctctc
      361 cgccgacggt tgcgggcact acgtgggcaa cctcctgtac atccacgaat ccaggaacgc
      421 attcagcatg ctggagggtg acgaggttat cgccgtggat ggggtgacga catgctacgg
      481 caccggcacc gaggacgcct acaacggcgg ctattactac aattgggtcg cggtgcagtc
      541 agacgaacca gagggtacct accctccctc ggctactcgc ccccttcatg gaatcatcta
      601 cgtgcgccgc gatgcgaccc cgatcgcccg ggcagaccaa taccgttggt acatcgccga
      661 ccgcgtgccg ttctgccagt caatccgggt tgacatcgaa tgccgttacg ccctcgtcgg
      721 ggccgagtac acgtccgtcg cattctggta tcagcttccg cccgtgcccg gaaactttga
      781 caatgactgc gacgtggatg ccgacgactt cgcccggttc gaatcctgtg cgaccggccc
      841 aggcgttcct ggttcagcgt ctcccgcctg caccatggtt cagttcgagg ccgccgactt
      901 cgaccacgat ggcgatgtcg accaacgcga cttcggcatc tttcagcttt gttacagcgg
      961 ggagaatgtg ctggcagatc cgaactgtgg aagctgatag tagaagctgc caacactgcg
     1021 atcgctgcgt ctgacccggc cgcaccggac atccagcggc ctgccgaggg atggccaaca
     1081 gagaaaagaa aaccggttcc gtcaccgaac atactacatc ttccgagcga ggtatctgcc
     1141 catgtcgacc gtcatgtttc ggcgttgccc cgaaaacccc attgtccgcc ccgggctcta
     1201 cccctggcga agagctgtca cgttcaaccc ggccgccctc tatgaaaacg gcaaagtgta
     1261 cctctatgaa cgtgcggctg gcgggctgcg accattccac tgctacatcg gacttctcga
     1321 aagcggcgat ggcgtgcact ttcgtcacgt cagcgaccag ccggtgttca cgcccgaaat
     1381 ggccggcaac cggtacggca cggtgcaaga cccccgcatc gtcaaactcg acggcctcta
     1441 ctacctcagt tttgccttcc gcccttacgc ctggaacatc tggcccaccg gtctgggcgt
     1501 ccccgaagcc gctcagccga cctttcccgg cttctccggc cgcgacgagg ataatcagac
     1561 gcgctcgggc atcgccgtct cgaccgaccg cctgcattgg aagcttcatt catgggtctc
     1621 gacgccggac atcgacgacc gcaacgtcat cctgttcccc gagaaactgg ccggtcgata
     1681 tgccgtgcta cgtcgcccca gcccctttgc aggcgtcacc accgagcacc gtagcgtcca
     1741 cccgggcatc aagatcagtt acagcgacga cctgcataac tggagcaccc cggcaaccgt
     1801 catcgaaccc gcctatcagt gggagaacaa ccggatcggc ggatccactc cgcccatccg
     1861 cacgccggcc gggtggctgg cgttctatca cggtgtggag aaccaggacc ccactgtccg
     1921 ccgcgtctgt tatcgcatgg gagcaatgct cctggacgcc gatgatccga ccaaggtact
     1981 tgcccgtacg cccaaacccc tgcttgagcc caccgagtac tatgagtgct tcggtctctt
     2041 catacccaac gtcgtcttcc ccaccggcgc ggtagtcatc gacgacgtgg tacacctcta
     2101 ttacggtgcg tgcgacacgg ccattgccct cgccacctgc cccctgagcg aactgctcga
     2161 ctacatgctg agtctgtagt gccccgcaac cttcacgggt gttccccttg atcgtttgcg
     2221 agattgcccc gtcctgaaag gatggagaat cgaatcggca aggaaatcgg acggcgactg
     2281 ttgacgcggc gcttgatccg gcgaaactgt cctgcctatg acccacacaa acctgcacag
     2341 caccgacccg accgcctctc ctggcatgcc ccaggttacg gccggaacta cggctccaag
     2401 ggtatcggcc ggacctcggc gggatttctc acctccgccc gcagataagg tgctgatcat
     2461 cggcctggac ggcgccacct tcgacgtcct caagccgatg atgaaacacg gacggatgcc
     2521 caatctcgcc cgactggtga aggaaggcac ctccggcatc ctatattcga cccagccccc
     2581 catcacgccc gccgcgtgga ccaccttcat gaccggcaaa gggccgggac gacacggcat
     2641 cgtggacttc gaacgttacg acgtcgcaac cggcaagctt tcattcaaca gcacctacga
     2701 gatccgcgaa cgcacaatct gggaaatcct cagcgaaaaa ggcttccgcg tgggcgccgt
     2761 ccacgtgccc atgacgtacc ccccgcggaa agtcaacggt ttcatgatct cgggttttga
     2821 aacgccgagc gtcgccgctg aattcacctg gccgccggag ctcaagcagg aaatcctgcg
     2881 acgatggccc aactacagtt atcgcgccga ttggcagcgg cgagtcttcg gtcgcgacaa
     2941 ggctctgcgt cgcaaccttc gtcatatcga acggagtttc caccaaggcg ccgagctgac
     3001 aacgttctgc ggcgagcggt tcggctggga tgtcctgatg gtgctgttca aactggtcga
     3061 caaccttcag cacaagacat ggaaatacct cgaccccaag acggccgtcc gatacccgca
     3121 cgaggccgaa ttggcggcca actgcttcgt cgaactcgac aaggctctcg gcatgctctt
     3181 tgattacgcc cgcaggtatt cggcgtcgat actgatgatg tccgaccacg gacacggcag
     3241 tctcgatggc aaagcccaac ccaacctgct gctcaaacgg tggggatatc tcaagatccg
     3301 aagcagcacc gtccagattc gtcgcaagct ttcgaaaatc gccgaccgct tcctgggccg
     3361 caggcgaaca cggtttgacg ccaacctcgg gatcgagcac gagctggtgg ttgactggga
     3421 acagacacgg gcgtgcgtca tccacgccgg catgaacggg ttcctctata tcagcctcaa
     3481 aggtcgtcaa ccccacggcg tcgttgagcc gtcggactac gagaaggtgc gagatgacct
     3541 gcgaaaccgc ttcctcgcgg ccaagtgtac cgatcccgcc ggccgggaag tacgcatctt
     3601 ctcggaggtc cacaaacctg aagagctcta cggatgcaac cgtgaggaac aaccgtggat
     3661 gcccgatttg ctgctcgtcc cacaacccgg tctggccgtc gtccggcgaa tcgtcggtcg
     3721 tcaaccagtg cgctggtcct cgttgcggcg acttgaaggc acgcaccgcg tcgaaggcat
     3781 ccttatagcc aacggtcccc acatccggca aggcagccaa accaccggtc gaatcgtcga
     3841 catcgccccg acgctgctgg cggcgctggg actcaaggtg ccgtctgaca tggaaggtaa
     3901 cgtgctgacg gacctctttg atcgaatgcc cactatcgaa tacgaactcc cgcagacggc
     3961 ggaatggaca cgacaggagt gcgtgtactc ggaagcagag aaggagatcc tgacaaagcg
     4021 actgaccgag ttggggtacc tggaatagcc gatgcatggc cctttcgccg cccctaaggc
     4081 cggatcattc aaagcaatcc ggcggaggta ccccgcccag cccgctgtag catcgctgaa
     4141 gcaccgcgaa gtcgcactgg tctacgtcgc catcctcgtc gagatccttt ccatcacagc
     4201 cggccatcgc tggaactcca ggagccgagg cgcagtcttc ccactctgcc atgtcgaatg
     4261 cgtcgacatc ctcgtcgcga tcgtagtcgc cggtgatcac cgcggaaatc ccgctgacta
     4321 acagggcgta gtattgaagg acgccgacaa gattaccctt gtgtgaaaca tgaacggtat
     4381 acgaacccgc gacaggacag gaaaccagga tctgctccac gttgtcccgc gtattgtcgc
     4441 cggtggtagc cggcgacgtc gggaacaacg gatcgaggac atacggcatg tggacggttc
     4501 caccatcggg agcgaccacc cggagatcaa gatcgttcac caggcacggt gaagggttat
     4561 ccagaccggt ccgcaccaca cccggcgggt cggtccagca aagcgtaacc ttgagtggac
     4621 gcacgccgtt cgacgcacac gtgtaggaga tggacggctg agccggactt aacagaccgc
     4681 tgactatccg ccgcgcggcc ggggaccgcc ggtgcaacaa caactgctcg gcagcagcct
     4741 cgacgttgag cagaccccag ccggtctgat agtccgggcc ggcggcctgt aaatcgtccg
     4801 ccgtgtgaat catgagccct ctcagcatct cagaccgcat atactgcccg ggaaacagcc
     4861 tgccgtagta ctcgaccagc aaggcggccg agccggagac cacggccgtt gccatgctcg
     4921 tgccgctgta ggttccgtac gaggcgtcgc ctgtcgccgt acaggagcga agagtcatcc
     4981 ccgaactcac gagatcgggc ttgattcgcc catcgtccgt cggtccccac gaggtgaacg
     5041 gtgtcacggt cgcaagcgac aacaaccgtt tccctccgta gacggcgtcc gatacggctc
     5101 cgacaaccat cacattcttg gctgtgcttg cgcttgccag ggtgtcgtag cctcccgcgt
     5161 cccaattatc cggtagcgga tcggtgttcg gatcgtatga tttctggcgc caccgaccat
     5221 tgaaccagta ctcgaacagg gcccccgccg cgggaacggg gtcgtttctc tcgttgcccg
     5281 cagccttgaa aggcaagtag taaggagcct catagcaaag ctcatcccat tgccgtgcca
     5341 cttcgtcgta ttgcccgaag tagctggact cccgctcacc ccacgtcccg tgccaccgga
     5401 aactgcccga gctgttgtcc cagccggctg cgtggacgta tgagtggttc gagatctgga
     5461 tcgcatccgg ctgtcccggc accgccatgg cgcgcgcggt catctccccc agatcactgg
     5521 tccagtcata ggaatcgatg cccgcgctcg gggccatgcc gatggcgctg ggatcaaccc
     5581 ccgtcgcacc aatggttccc gccacatgcg tggcatggtc gaaggaagag ccgctgtccc
     5641 gaatcgacac ccgaccggtc agctcccgat gggtggcacg aaccgcgcca ccgtcccaaa
     5701 ccccgacgac aacgccgaag ccgttgatgt cgtacggggg agtatttcgg accagatccg
     5761 ccgctgccgt aatgccggca tcgctgttag ccgtttggca gatgtacact ctgccgtcac
     5821 gaatgcccat gagctcgctg acaacctggc cgtgctgtgt ccggggcgac catccgtgcc
     5881 tttgcgcgga agcccaagcg gccttcttgg cctcgagtgc ctgagtggcc aagagccgtg
     5941 ctttttcggc tctgcccctg agtgccccgt catccggcaa cgactgcggc tgggcctgca
     6001 agccacccga gctcagcagg caaagcccgg ccgcgatcca aaggatagtg aaggtccgca
     6061 tctcttcccc acgcttagcc atgccgccgc aggcaagctc acctctccac gatgccaggc
     6121 gcgtgcaacc tcggcaaacc gcccccgcaa acgagccgcc ccccttggga ttgtctctga
     6181 tcttattata gccgcaggta catcagaccg aaccgggaag gctcaagccg gccctctccg
     6241 tgcgattcca gaaagcatcc gatcagcaaa acctgaaggg gccacgccta cagtgctctc
     6301 taaggcggac cgcaacccaa gcgctctgca atccagaaac atgcccgcaa gccgcacgca
     6361 tatccggccg taaggttcta aggcggaccg cctttttgcg ggccacaacc caagcgctct
     6421 gcaatccaga agcatgcgcg caagtcgcac gcatatccgg ccgtaaggtt ctcatacccc
     6481 tacgacgccg acgtcggacg ggtggacgct ccgagtctca ggacgtaccg tcgctccttc
     6541 acgtgtcccc tgctatgccc ccccgccctg cgggacgaat gtcaatcgtc gcacctgcga
     6601 catgccatgg ctcgcattcc cggaccttgc atgatcgccg cgcagcgcga cccaaggacc
     6661 aagctttgcg gccgtcagca gcctcgccgc ctcacgacca gccgcaacac gcagagcccg
     6721 accagcccca cggccatcag ttgggcggcc ccggctccgc atggaacggc acgccccgat
     6781 gaaggcgcat ccagcgccgg ctcctcgttg gtcggaaccg ccgtctggtt atcggccggc
     6841 atgatctgat ccttctttcc agccgaagat acggaggcat cgcaagcgtc gccgatcccg
     6901 tctccgtcct ggtccaactg gtcagcgttc gcaacgcgcg ggcagttgtc gcaggcatcc
     6961 ccgacgccgt caccgtctga gtccgcctgg tctgcgttag gaacgccgac acagttgtcg
     7021 cacatatccc cgatgccgtc gtcatcggca tcatcctgag tgggatcgta gatgttcggg
     7081 cacttgtcac aagcgtcgcc cacgccatcc ttgtcactgt ccttctggtt ggcattcgcg
     7141 accgtcgggc agttgtccag gcagttaagc ttgccgtcct tgtcatcgtc cgcatccggc
     7201 acaccacagc cacaaacgcc cggctgcacc ttcttcgggt ccacgtcgca ggtgtcgtgg
     7261 cagtcgggcg tgccgtcgcc gtcccggtcg cgatcgagca ccccgcaacc acacttgccg
     7321 ggcacgctct tgtatgggtc gttgggacat tcgtcgacgc agtcaacctt gccgtcgaaa
     7381 tccacgtctt tctcgagcag gccgcagcca cagagccccg gatcctgctt gttggggtca
     7441 ttcggacagt tgtctatggg gcaagtgttg ttcgggaacc ccgggtcgcc gaacctgtcg
     7501 ccgtcggaat ctgtgcacgt gtcgcacgcg tcaccccaaa gatcgtcatc ggcattggcc
     7561 tgatccgcat tcgcaatgtt cggacagttg tcctgcggac aggtgttggc cgcaaacccc
     7621 ggattgcctg tgccatcacc gtccgtgtcg gtacaggtgt cgcagacgtc gccgatcccg
     7681 tcctcatcct tatcccgctg ggcagggttg ggaatctcgg gacagttgtc gcacgcatcc
     7741 ccgagcttgt ccgagtccga gttggtctgg tccggattgg ccaccttcac gcagttgtcg
     7801 caggcgtcac ccacggcgtc accgtcggca tcctgctgaa agggattcac cgcgctgacg
     7861 cagttgtcgc acgcgtcacc gatgccatcc ttatcatcgt ccgcctgatt cgggtttgcg
     7921 atcaacggac aattgtcgca ggcatcgccc acgccgtcac cgtcgtcgtc atgctggtcc
     7981 gggttcagcg tcagcgggca gttgtcgcag gcgttgccca gaccgtcacg atcagagtcc
     8041 gtctgatccg gattcggaac aagcaggcag ttgtcgcagg catcacccgc ggcgtcgccg
     8101 tccgcgtcgg cctggtcttc gttcgctacg acaggacagt tgtcgcaggc gtcgcccagg
     8161 gaatcactgt cgcggttccg ctggtccggg ttgggcaccg tcgggcagtt gtcgcagact
     8221 tcgcctatcc catcaccgtc cggatcgggc aagccgttga cctggacgac gaattcgatc
     8281 tcgtcatctc cctcggcggc cgttacggac aacagcaacg gaatagcctc acacatggga
     8341 tgctgggcct taacctggat cttgaaagga ccggcgtttg tgcccacccc acccgtcggc
     8401 aggtccggat agctctgagt agcgttggct cccacgacgg tcaccgtccc cacgtcgcat
     8461 gaaagcgtcg ccgagacatt cgtggcgtcg ccgccgacgt tcttcagggt gatcgtgagg
     8521 tcgacggtct cacccggttc caccgaaccg tcgccgtcgc cacccgccga gtcgtcaatg
     8581 acggggcggc cgtccaattc gaaactcggc ccgctgtgac aggcaaatac ctcgaaatcg
     8641 tccaaatacc accccacgtt ggtgccgtag aacgagtcgc ggccgaagtc aaaccggaat
     8701 tgcactttgt cgccggcctt gacgctgccg ggaagggtga acgccgactt gccccatccg
     8761 ccggaggaac cggtccagac gatctgaccg gccatgggat tgtcgttgga ggaatagtcg
     8821 gctcgtgcca ggctgctggt gtacgagttg tgaatgaaag acgtgaccag cgtccaagca
     8881 ccgctgttga ttctcatgct gatgttgccg ccgtcccacc attcctcgac atcgaaatag
     8941 tgagcaaata ccactccgag tcccatcact cccggaggaa tcgtgatctc cggactggtc
     9001 agcgtctgtt tcgcggcacc gctcgggttg gacggatcgg gacagtacca tcccttgccg
     9061 gaccgaccgg gcataccgga tttccactgc cactcgctgt ttccacgcga ccagcctccg
     9121 tccgtgctct cgaagctctc agaatagagt gtcacccggc ctccacaaag gacaggctct
     9181 tcgcgagaga agaaatcgcc gcaccggccg ggcatgtcca tctgaaccgc ccgaagggca
     9241 ttgtccacct gctgcgcatc cgcaaccgtg aaagtggaat ccgaaggcaa gccggtccgc
     9301 ggatcggccg gcttggtccc gatcagatcc tgagccgcct ggttgaatgc atgataggca
     9361 tcctggaaag cagagatcgt tgtcaggtat ctggtcagcg cccggtacca gaccgcgccc
     9421 gtcttgatcg gtccgatgcc cgtcaccgtg atacc
//
LOCUS       DAOTPW010000226         9424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6695_length_9424_cov_6.242502, whole genome shotgun sequence.
ACCESSION   DAOTPW010000226 DAOTPW010000000
VERSION     DAOTPW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6695_length_9424_cov_6.242502"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="P5316_17260"
     CDS             complement(<1..651)
                     /locus_tag="P5316_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR86762.1"
                     /translation="MKELTPRQIVEALDQYIIGQDEAKRAVAIAVRNRWRRQQLPENM
                     RSEVGPKNILMIGPTGVGKTEIARRLARLVNAPFVKVEASKYTEVGYHGRDVESMIRD
                     LLDLAIHMVRAEQTEVVRQEAERIAEERLIDALLPAGTGDEDDDPNSDTAQKRHRTRE
                     KFRRKLRDGEFDDRDIEITAEERPMPAGMMATFGVDQLDPEIQSFFEKLIPQQRKNR"
     gene            789..1565
                     /locus_tag="P5316_17265"
     CDS             789..1565
                     /locus_tag="P5316_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86763.1"
                     /translation="MIAPGSPSEEGFCAKCRYPLRGLPEPRCPECGSEFDPANARTFL
                     VATQRWALGPVSRRLWNPPTWKLHALSAVPALLILFSYSVPGQVTVFRDLGTLIAMVL
                     LVSWLLRLILSLAVEIIYRPPLDQRRAPGPWLLTPLIIFVTAVLCRLDVPAHVAFALS
                     RPSLDRLAARAATTPLSPPLMDRWVGVYPIKEIRRIHGGMRIWVWGTYGLGGGFAWFP
                     QGPPVSNEYVYFHWCDSWYGWHDMARGGRSLASGDLWHFR"
     gene            1844..2128
                     /locus_tag="P5316_17270"
     CDS             1844..2128
                     /locus_tag="P5316_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86764.1"
                     /translation="MRNAPLRFEGFVLRSGRKYESICPDLDVASFGKTPADARKALLE
                     ACRLHVESAIECNLPYLRPVPPDDHPLREEPSRIVERFMVTIEIAVKAHA"
     gene            2121..2339
                     /locus_tag="P5316_17275"
     CDS             2121..2339
                     /locus_tag="P5316_17275"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRR86765.1"
                     /translation="MPELPVLTARQVIQALSVLGFTTLRQRGSHLQMRRGNRMATVPL
                     HRGDIPPGTLKSILRQAGITVQELIDSL"
     gene            2372..3025
                     /locus_tag="P5316_17280"
     CDS             2372..3025
                     /locus_tag="P5316_17280"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRR86766.1"
                     /translation="MPNGTCYEARPIILASQSPRRVDLLREAGIAFEAVAPTCVEPDV
                     ATWMRSPEEFVQLAAYQKVSSVAERYPDRVILGADTVVALNGRMYGKPADRDDARRIL
                     SALAGVTQDVITGVTILQPAKGRQLVECDVTRVTMRPMSEEEIEVYLDSGEWEGKAGA
                     YGIQGTADRFVEKVEGSFSNVVGLPVERVLRMLAEFDIRPTYGFSRQPRVSDAQECE"
     gene            3022..4728
                     /locus_tag="P5316_17285"
     CDS             3022..4728
                     /locus_tag="P5316_17285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HRR86767.1"
                     /translation="MIPATWIVIVASSFAANEYSPLTAGELLLVYNDADVSSRTLAKH
                     YAELRDVPRDQLCPLVIMRVGEEIAPADFERLIREPVRRYLEEHGLRDKVRCLVTFYG
                     LPIRVGRQTPSVAQQQLLVKWRPRLKREIREFEAMLRQLESVAGLASRPAEVRPPDEN
                     DLPRLLQQYVKAREAAIKLMAGPSAQPENEERRRNWLELMQKAEGQAFVATHLQVVSG
                     DGDQAVRRQLERLRESVSADDARVQQILAEGIESSSREEAWQILQRNRGLLALLSAIQ
                     RDIQSIQPEETEASVDSELMLLWWRDYPRYRWVVNTLNWQRRAAAEQQVAQYEPPWLW
                     RTLMVSRIDAGSAAVARRMIDQAITIEKVGMTGKFYIDARGLKPDGNYGDYDESLRKL
                     ARMLQNAGSIPVVLDNRADVFQPGQCPQAILYCGWYSLRKYVPAFTFVPGAVGFHVGS
                     FEAASLKGPKETGWCKNLLDDGVIATIGPVAEPYLNSFPQADRFFGLLLTGRYTLAEC
                     YAYTLPFNSWMQMLLGDPLYRPFARSPVLTVEDVYDPREIPRAFRQRATSQPATDSAD
                     NK"
     gene            complement(4767..7493)
                     /locus_tag="P5316_17290"
     CDS             complement(4767..7493)
                     /locus_tag="P5316_17290"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HRR86768.1"
                     /translation="MKDEQIIRRLCLIAVLLSMASSRLAAQTTSPAQVKEVVVVCKTH
                     FDIGYTDLVSNVLDYYRTTMIDKALAVCDQTRDLPPEQRFVWTLPGWPLSHILGPLQT
                     DARRERIVAMIRESRLVWHALPGTTHTESLELEDLVRGMGFSSRLARQFGQELPRDAK
                     MTDVPSHAWALSTILAQAGVTFLHLGCNRLSTPPDVPVLFWWEGPDGSRVLTMQSPAY
                     GTGLTPPADWSHAAWLALIHTGDNHGPPTPEQIQELLAQAKSELPDVKIRMGRLSDFA
                     DAILAEKPDLPVVRADMPDTWIHGIMSMPVESGLARRVRPLIGATESLGTLLDLWRVG
                     DVDMSGTIAEAYEQSFLFGEHTWGLDGKRFPRLYGQAWQDEYDKGTFVKLEQSWGQHA
                     AYIRRVEELIRPTLGEGIRALAAAVNVEGTRIVVFNPLPWSRDDVVSLPWSQATVGKV
                     RDLETGRSVPAELKDEQLSFVAHGVPPLGYRTFVLDSAGTVSPTGKTAWQRADSFDAL
                     QVVENPRWRVRIDTTRGTITSLYDKQSDQNLAGEIDGIGLGQFVYERYGADDVSQFLD
                     VYTRGKAPWLEGDFGKPGMPPADQVPHSVFSPPRFSHTIDHGPVSTRIILAAQPSELF
                     NGRVSLTIRLYEDLPFVDFEWKVSDKQATPWPEAGWLCLPLRIDQPVFRLGRLGCVVD
                     PARDVQPSANHMVYALTTGLTLTAPKGKGAGICPIDSPLVCLDERGLWKFSRDFVPKR
                     PMVYVNLFNNQWSTNFVQWIGGAWSCRVRLWPISGTAEEDALVTPAMEARVHCQAILV
                     KGKAGTLPPARAGLTLSRKGVLVTALGPNPDGHGLLLRLWEQIGSDQPCRVQLPDGLR
                     PTSVLPCDLRGRPSGEPIPVKEGVFEVPMTRFAPLSLLIAEQEQAGPTLREE"
     gene            complement(7504..8328)
                     /locus_tag="P5316_17295"
     CDS             complement(7504..8328)
                     /locus_tag="P5316_17295"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRR86769.1"
                     /translation="MTDKQAANGRLDLMRRVRVVLVRPQVAGNIGAVARLMENFDAGQ
                     LVLVEPKASAVSREALQRSTHGEHRLTSARIVPTLSEALDGVVYAVGASRRRGPVHQA
                     DDLTPPAMARVLAEHVGEGDVAILFGTEDNGLSREDLVNCDAVVNIPASPAYGTLNLS
                     HAVAICLYETFVAVAGAPEPKGHPKRCLDPADLKLMNRLMIKLQHALLTIGYLRPEKP
                     DHLMFPIRNILSRARLTRAEAQILIGLAQQIDEFAVYGKKSAPAGPSKGSGFVEKG"
     gene            complement(9237..>9424)
                     /locus_tag="P5316_17300"
     CDS             complement(9237..>9424)
                     /locus_tag="P5316_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86770.1"
                     /translation="TTYSNRRNLCTFRTTLANTLTTPEMPSQCAGESGHRFADQSQRV
                     AGYGDKKKEVPPRTRTI"
BASE COUNT         1700 a         2925 c         2935 g         1864 t
ORIGIN      
        1 gcgattcttg cgctgttgag ggatgagctt ttcgaagaaa ctctggatct ccggatcgag
       61 ctgatcgacg ccgaaggtgg ccatcattcc ggccggcatg ggccgctctt cagcggtgat
      121 ctcgatgtcg cggtcgtcga actcgccgtc ccgcagcttc cggcggaatt tctctctcgt
      181 gcggtgacgt ttctgggccg tatcgctgtt cgggtcatca tcctcgtcgc cggttccggc
      241 gggcaggagg gcgtcgatca ggcgctcctc ggcgatccgc tcggcctcct ggcggacgac
      301 ctcggtctgt tcggcgcgga ccatgtgaat ggccaggtcg agcaggtcgc ggatcatgct
      361 ttcgacgtcc cgcccgtggt aaccgacctc ggtgtacttg ctggcctcga ccttcacgaa
      421 cggggcgttg accagcctgg ccagccgccg ggcgatttcg gttttgccca cccctgtcgg
      481 cccgatcatg aggatgttct tggggccgac ctcactgcgc atgttctcgg gcaactgttg
      541 gcgccgccag cggttccgca cggcgatggc cactgcccgt ttggcttcgt cctgaccgat
      601 gatgtattga tccaaagctt cgactatttg tctgggggtc agctctttca taactcgcgt
      661 gtattgctcc tgtgacgatc ttcaacttat cgccggccga cattctaccg gaaaccgggc
      721 tcatagagca gtggccgggg gagcgtcgat gattcgcaga caccatccgc aagcagtatc
      781 atgctctcat gatcgccccc ggcagtccga gcgaggaagg cttctgcgcc aaatgccgct
      841 acccgctgcg cggcctgccc gagccgcgat gcccggaatg cggtagcgag tttgatcccg
      901 cgaacgctcg cacatttctt gttgcgacac aacgatgggc cttggggccg gtctcccggc
      961 gtctctggaa ccctccgaca tggaagcttc acgccctgtc cgccgtacca gcgttgctga
     1021 ttctgttcag ctattcggtg cccggtcaag tcaccgtttt tcgggatctc ggcactctta
     1081 tcgcgatggt cctgctggtg tcgtggctgt tgcgcctcat tttgtcactg gctgttgaaa
     1141 tcatctatcg ccctcctctc gatcagaggc gtgccccagg gccatggctg ctgacgccct
     1201 tgatcatctt cgtgacagcc gttctctgcc gcttggacgt gccggcacat gtggcgttcg
     1261 ctctcagcag gccgtctctt gatcgcctcg ccgcccgagc ggccaccaca ccattgagtc
     1321 cgcccctcat ggaccggtgg gttggcgtgt atcccatcaa agaaattcgc agaatacacg
     1381 gcggcatgcg gatatgggtt tggggaacct acggcctcgg cggcgggttc gcctggttcc
     1441 cgcaagggcc gcccgtcagc aatgaatacg tctactttca ttggtgcgat tcctggtacg
     1501 gctggcatga catggcacgg gggggacggt cactggcatc cggagacttg tggcatttca
     1561 gatagtgcca ttgggctgcc cgttgtcggg ccaccgagcc gaatggatcc ccgacactct
     1621 gggcggctcg gttggtggct tcgttggcgg tgggtctctg gtacaggcca gagaccgagg
     1681 acacgaaatc cgcaatccgt tgggcgttgc cacccgggtt gtttgctttg gcaggtatcc
     1741 tgtatgcgtt gaacgttcca ctgttcgtgg tatttgtgtt gccgagccgt gcccgtgcca
     1801 gggatggcta gccgcttctc agcagatttg atacactgga agcatgcgaa acgcccctct
     1861 tcgattcgaa ggcttcgtgt tgcgatccgg ccgcaagtac gagtcgatct gccccgacct
     1921 cgatgtggcc agcttcggca aaacgcccgc cgatgctcgg aaggccctgc tggaagcctg
     1981 tcggcttcac gtggagtcag cgatcgagtg taatcttccc tatcttcgcc ccgtgcctcc
     2041 tgacgatcac ccattaaggg aagagccgtc tcgcattgtt gaacgcttca tggtcacgat
     2101 cgagatcgcg gtcaaagctc atgcctgaat tgcccgtgtt aacagcgaga caagtgattc
     2161 aagccctatc agtattggga ttcaccacat tgaggcagcg gggcagccac ctgcagatga
     2221 ggcgggggaa ccggatggcc accgtacctc tccatcgagg tgacatcccg ccggggacct
     2281 tgaaatcgat tttgcgacag gccgggatta ccgtacagga gttgatcgat tctttgtaag
     2341 aggcgattgt tcgatacagg tgccgatgcc aatgcccaat ggcacgtgct acgaagcccg
     2401 gccgatcatc ctggcctcgc agagtccgcg gcgggtggat ctgctgcgcg aggcggggat
     2461 tgcctttgag gcagttgcgc cgacctgcgt cgagccggac gtcgccacat ggatgcgcag
     2521 tccggaggag ttcgtccagt tggcggcgta ccagaaggta tcgagcgtag ccgagagata
     2581 tcccgatcgg gttatcctgg gggccgacac ggtggtagcc ctcaacgggc gcatgtacgg
     2641 caagccggcc gaccgtgacg acgcccgacg cattctttcg gcgctggccg gcgtgaccca
     2701 ggacgtgatc accggcgtga ccatcctcca gcctgccaag ggcaggcagc ttgtcgagtg
     2761 cgacgtgacc cgggtgacca tgcggccgat gtccgaagag gaaatcgagg tctatcttga
     2821 tagtggcgag tgggagggca aagcgggggc ctacggtatc cagggcacgg ccgaccgatt
     2881 cgtcgagaaa gtggaaggca gcttcagcaa cgttgtcggc ctgccggttg aacgggtgct
     2941 tcgcatgctg gccgagttcg acatccgacc gacatacggt ttctcgcgtc agccacgggt
     3001 gagtgacgct caggagtgcg agtgattcct gcaacatgga ttgtgatcgt tgcctcaagc
     3061 tttgctgcga acgagtactc gccgctgacg gcgggtgaac tgttactcgt gtacaacgac
     3121 gcggatgtca gctcgcgaac cctggccaag cactatgctg agctccggga cgtgcctcgc
     3181 gatcaactgt gcccgctggt gatcatgcgg gttggcgagg agatcgcccc tgccgatttc
     3241 gagcggctga tccgtgagcc agttcgccgc tacctggagg agcacggtct tcgggacaag
     3301 gttcgttgcc tggtgacctt ctacggtctg ccgattcgcg tcggtcggca aacaccgtca
     3361 gttgcgcagc aacagttact ggtcaaatgg cggccgcgac tcaagcggga gattcgcgag
     3421 ttcgaggcga tgctgcggca gctcgagagc gtggccggac tggcttcccg tcccgccgag
     3481 gtgagaccac ccgatgagaa cgatcttccc agactgttgc agcagtacgt caaggcccgc
     3541 gaggccgcaa tcaagctcat ggccggtccg tccgcgcagc ctgagaacga agaacgccgc
     3601 cgcaactggc tggagctgat gcagaaggcc gaaggacagg cgttcgtggc gacccatctg
     3661 caggtggttt ccggagacgg tgaccaagcc gtacgccggc aactcgaacg gcttcgcgaa
     3721 tcggtttccg cggacgacgc tcgggtccag cagatacttg ccgaagggat cgagtcgtct
     3781 tcccgcgagg aggcatggca gatactccag cgcaaccgcg gcctgcttgc cctgttgtcc
     3841 gcgatccaaa gggatattca gtccatccaa ccggaggaaa ccgaagcatc cgtggacagt
     3901 gaactgatgc tcctgtggtg gcgcgactat ccgcgatatc gctgggtggt caacacactg
     3961 aactggcaac ggcgggcggc ggccgagcag caggttgcgc agtacgagcc gccgtggctc
     4021 tggaggacgc tgatggtcag ccggattgat gccggcagcg ccgccgtcgc ccgccggatg
     4081 atcgaccagg cgatcaccat cgagaaagtt ggcatgaccg gcaagttcta catcgacgcc
     4141 cgggggctca agcccgacgg caattacggt gattacgatg agagtcttcg gaagctcgcc
     4201 cggatgttgc agaacgcggg aagcatccct gtcgtgctgg acaatcgggc cgacgtgttc
     4261 cagcccggcc agtgtcctca ggcaattctc tattgcggct ggtacagcct gcgcaagtac
     4321 gtgcctgcct ttacgttcgt tcccggggcg gtgggctttc acgtcggcag ctttgaggcg
     4381 gcgagcctca aaggccccaa ggagaccggc tggtgcaaga acctgctcga cgacggcgtc
     4441 attgccacca ttggcccggt agccgagccg tatctgaact cgttcccgca agccgaccgt
     4501 ttcttcggcc tgttgctcac cgggcgctac acccttgccg agtgctacgc ctacacgctg
     4561 ccgttcaact cgtggatgca gatgctgctg ggcgatccgc tataccggcc gtttgcccgc
     4621 agcccggtgt tgacggttga ggatgtgtat gatccgcggg aaatcccgag agcgtttcgg
     4681 cagcgtgcga ccagtcagcc tgccaccgat tccgcggaca ataagtaggc cgcaacgtcg
     4741 tcggttctct gccggccgct tcctgatcac tcttctcgca acgtcggccc ggcctgctct
     4801 tgctcggcga tcagcaggct cagtggggca aagcgggtca tcggcacctc gaagacgcct
     4861 tccttcaccg gaatcggttc acctgaaggc cgcccccgca gatcgcacgg caggacgctt
     4921 gtgggccgca acccgtcggg cagttgaacg cggcagggtt gatcgctgcc gatctgctcc
     4981 cacagacgca gaagcagccc gtggccgtcg gggttcggtc ctaacgcggt caccaggacg
     5041 cccttgcgcg acaaggtcag gccggcgcgg gccggcggaa gggttccggc ctttcctttg
     5101 accagtattg cctggcaatg aacgcgggcc tccatcgccg gagtgacgag ggcgtcctcc
     5161 tcggccgtgc ccgaaatcgg ccacagacgc acgcggcacg accatgcgcc gccgatccat
     5221 tgcacgaaat tcgtggacca ttggttgttg aacagattca cgtacaccat cggtcgtttg
     5281 ggaacgaaat cccgcgagaa cttccacagg ccgcgctcgt cgaggcagac cagcggtgaa
     5341 tcgatcgggc agatacccgc gcctttgccc ttgggggcgg tgagggtcaa accggtggtc
     5401 agcgcataga ccatgtggtt ggccgatggc tgcacgtcgc gggcggggtc gacgacgcac
     5461 ccgaggcgcc cgagccgaaa cacaggctga tcgatccgta aaggcaggca aagccatccg
     5521 gcttcgggcc acggagtagc ctgcttgtcg gagaccttcc attcgaaatc gacgaacggt
     5581 aaatcctcgt agaggcgaat cgtcagactg acgcggccgt tgaaaagctc ggacggctga
     5641 gcagcgagga tgatccgcgt cgagaccgga ccgtggtcga tggtgtgcga gaagcgcgga
     5701 ggcgagaaca cgctgtgagg aacctggtcg gccggcggca ttccgggttt gccgaagtcg
     5761 ccttcgagcc atggagcctt gcctctcgtg taaacgtcga ggaattgcga gacatcgtcg
     5821 gcgccgtagc gttcatagac gaactgacca agcccgattc cgtcgatctc gccggcaagg
     5881 ttctgatcac tctgcttgtc ataaagcgaa gtaatggttc cgcgcgtcgt atcgatgcgg
     5941 acgcgccatc tcgggttttc gacaacctgc agagcatcga acgaatccgc cctttgccat
     6001 gccgttttgc ccgtgggcga aacggttccg gcactgtcga gcacaaaagt gcgatagccg
     6061 agcggcggga ccccgtgggc gacgaaagag agctgctcat ccttcagttc cgccgggaca
     6121 gaccgtccgg tctccagatc gcggaccttg cccacggtcg cctgcgacca cggcagactg
     6181 accacgtcat cccgtgacca cggcagcggg ttgaacacga cgatccgggt tccttcgacg
     6241 ttgacagcgg cggccagtgc ccgaatgccc tcccccagcg tcggcctgat cagttcctca
     6301 actcgacgga tataggctgc atgctggccc cacgactgtt caagcttgac gaaggtgcct
     6361 ttgtcgtact catcctgcca tgcctgccca tagaggcgcg gaaaccgctt gccgtccaag
     6421 ccccaggtgt gctcgccgaa aaggaagctc tgctcgtaag cctcagcgat cgtcccgctc
     6481 atgtccacgt caccgacgcg ccacaggtca agcagcgtgc cgagcgattc ggtcgccccg
     6541 atgagcggac gcacgcggcg ggccaggccg ctctcgaccg gcatcgacat gatcccgtga
     6601 atccaggtgt cgggcatgtc ggcgcggacg accggtagat ccggcttctc tgcaagaatg
     6661 gcatcggcga agtccgacag ccggcccatg cggatcttga catcgggcaa ttcactcttg
     6721 gcctgtgcca acagctcttg tatctgctcg ggcgtcggtg gtccatggtt gtcgcccgtg
     6781 tggatcaggg ccagccaggc ggcgtgcgac cagtcggccg gcggcgtgag gcctgtgccg
     6841 taggcgggag attgcatggt gagcacgcga ctgccgtcgg gcccctccca ccagaacagc
     6901 accggcacat ccggcggcgt gctcaggcgg ttgcagccca ggtgcaggaa ggtcacgcct
     6961 gcctgggcca ggatcgtgga cagcgcccag gcgtgcgacg gcacgtcggt catcttggcg
     7021 tcgcggggca actcctgacc gaactgccgg gccagacgcg acgagaaacc catgccgcgg
     7081 acgaggtctt cgagttcgag cgactcggtg tgcgtcgtgc ccggcagagc gtgccagaca
     7141 aggcggctct cgcggatcat cgcgacgatg cgctcacgcc gcgcatccgt ctgcagcgga
     7201 ccgagaatgt ggctcaacgg ccagccgggc agagtccaca cgaaccgctg ctcgggcggc
     7261 agatctcgcg tctggtcgca aaccgccaac gccttgtcga tcatcgtcgt gcggtagtag
     7321 tcaagcacgt tggacaccag gtcggtatag ccgatgtcga aatgcgtctt gcagacgacg
     7381 acgacttctt tcacctgtgc cggcgaggtt gtctgggcgg ccagtcgcga actcgccatc
     7441 gaaagaagaa ctgctatcag gcagagacgc cgaatgattt gttcatcctt catccttccg
     7501 ccttcatcct ttctcaacga agcccgaacc ctttgatggc cccgcaggcg ctgacttctt
     7561 tccgtagacc gcaaactcgt cgatctgctg cgccagcccg atgagaatct gtgcctcggc
     7621 ccgcgtcagc cgtgcccgcg agagaatgtt tcggatcgga aacatcaggt gatcgggttt
     7681 ttcgggcctc aggtatccga tagtcagcag agcatgttgc agcttgatca tcagccgatt
     7741 catcagtttc aggtcggccg ggtccaagca ccgtttcggg tgccccttcg gttccggcgc
     7801 gccggccacg gccacaaacg tctcatacag gcagatggcc actgcgtgcg agagattcag
     7861 cgtgccgtag gcagggctgg ccgggatgtt caccaccgcg tcgcagttga ccaaatcctc
     7921 acgcgacagc ccattgtcct cggtcccgaa caggatggcg acgtcgcctt cgccgacgtg
     7981 ctcggccagg acgcgggcca tcgccggcgg agtcagatcg tcggcttgat gaaccggccc
     8041 gcgccgtcgg ctggccccca ccgcatacac cacgccatcc agcgcctcgc tcagcgtcgg
     8101 gacgatccgg gccgaggtca gccggtgctc gccgtgcgtg ctgcgctgca aagcctctct
     8161 tgaaaccgcg ctggccttcg gctcgaccag cacaagctgc ccggcatcga agttctccat
     8221 cagccgggcg accgccccga tgttgccggc cacctgcggg cgcaccagga ccacgcgtac
     8281 gcgccgcatc agatcgagac gaccgttggc cgcttgtttg tcagtcatgg cttccgtcac
     8341 cggcggtgat tctactcgaa ccggccaaga agtgtcctca ccccacagtc ggcccatctg
     8401 tcgcgattcc gcgtccggcg atcctggtcg tgtgccgatg cttcttgaac ctgttgtcac
     8461 cgacatcggt caggagacga gaacgccggc acaactgatg cctcggttgc acaagaatct
     8521 ccgcaggtct gcagaagagt acgtttggag cgggcgtttc gcgctgaaat acggctgccg
     8581 ccggatctca ccggcggcga cgttcaaggg cgacgacaga acacggccca ttctcaattc
     8641 cacattcaca attcttcatt ccccggcgtt gcagtatgca acgctggggt taaaacaccg
     8701 cccctctggc tgctgacagc cgaatgctga ccgctgaatg ccgagtgccg accgtacctg
     8761 ccccagcggc ccgtcaccac caaaccctga ggagtcttca catttcaacg atcgttcgag
     8821 aagactcaac tacacccttg acgttctata gcgggtcttt tccgggccct tttcctggcg
     8881 cgtccacagt ctgccctccc gcaaagccgg cgggggtcct tacgcaccag ggagtaattc
     8941 tttagccaag gcttgacacg cggaggagcg taccggtaat atgcatatgg ttcaatgcgc
     9001 cagtccggct tgcgggaatc ccaccggccg ggccgtgaga ggaggcaaga acagggccaa
     9061 gagagcccga acggaacgta cgtagctgat tgaatatcag cgttacagac ttgggcgctt
     9121 atctgaaaac cgccagccag ggttcttcct ggaaaatgcc aaaacgcaca gggctgggcg
     9181 cactcccgca tgggagtgtc gtcctggtcc gtgcttggcg tgggtctggc gacccttcag
     9241 atagtgcggg tccttggcgg cacttccttt ttcttgtcgc cgtatcccgc cacccgctgc
     9301 gactgatctg cgaaccgatg acctgattct ccggcgcatt gcgacggcat ttcgggagtg
     9361 gtgagtgtat ttgccagcgt cgttctgaaa gtgcagagat ttcgccggtt tgaatatgta
     9421 gtct
//
LOCUS       DAOTPW010000227         9421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6697_length_9421_cov_5.244928, whole genome shotgun sequence.
ACCESSION   DAOTPW010000227 DAOTPW010000000
VERSION     DAOTPW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6697_length_9421_cov_5.244928"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="P5316_17305"
     CDS             complement(<1..1475)
                     /locus_tag="P5316_17305"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR86771.1"
                     /translation="MRRSHLILVAVTAWSVSFAGAQATDDALKDYTWFLNKLIDLDHL
                     VVLEHGQTCKQFSSYDRASRYDEASDQYVKWDANGDAGHYLRIDPATGEGVMAEMEGP
                     GCIWRIWSANPQGRIRFYFDGAEKPQLEHEFSALFTGKVTGFPKPLVWQRRTDVGGDN
                     PASNCYVPIPYAKSCRVTADKPVNQYYHIGYTTFPKEWRVKTFSLEHTPAEEIVFQRV
                     CKALANCGVDPQPVQNALTIEKNTTLVPGQPIPVADLKGPGTVRQFLTKLTSEERWAR
                     RKVLLQIFFDGDAAPAVEAPIGDFFGDAWDEASYKSLPLGITDDLNYCYWRMPFAQSA
                     RFVVTNQGRRPAQLRTRIVYQQGGPPAGAARFHAKWRRDPVSKDFDYPFLECAGPGRF
                     VGAALFIDNIAGGWWGEGDEKVWVDGEKFPSTFGTGSEDYFGDAWGIRHFANAFHGCP
                     TPGEFGQRRRQSCYRWHVADSIPFDNSFKMTIENYAAITKPP"
     gene            2057..2836
                     /locus_tag="P5316_17310"
     CDS             2057..2836
                     /locus_tag="P5316_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR86772.1"
                     /translation="MRTHSGHRGFTLIEMLVVVAIIALLVAILLPALEGARRNAKSVV
                     CLSNLMSIGSAMKLYQGDYHGWLPVGPADKLWYIDSTLGTVREPAPGRRPFPWSNCHW
                     GGKRAAWIHDVENDPPKPEVLGRPLTRYLYRGTGLDQPTPLFECPADVGDPMIENPVG
                     QLPVHYLCGNSYYTNPWNRYKPPGRRRPSPSSIVLVEDAPMYMDLVHKQQRMGWHGRF
                     SVHNALFLDFHAEAKYMDTRTFVGPGWFVENYFNIMDYYKY"
     gene            3032..4252
                     /locus_tag="P5316_17315"
     CDS             3032..4252
                     /locus_tag="P5316_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86773.1"
                     /translation="MTTTRPAAGVPEPPEVREQVDAARRGLAKAMLQQDANAVESAVA
                     RMHEALGEWATVPETAPRFAGSPNPDVAVAPDRLRRLWTAGWAAADKAYSGDGRWGLP
                     RDHPEVRHTLLRQTAYVIIGGIAAMRQGFGDEAAIRKRVKEGLDYLLSVQKPNGLFPF
                     PDVRGWHPHFSPPLQSLYAQHPEAFQDGWIIDDFGDGGLQFDNGVCAVTMLTAFEHFK
                     TTAYRESAERACRWAVERPVVPNWNYNAFSVWALARCVEAVGREDFRAPAIQKTVLGI
                     LPGQLPAGRWLDRHNCRTVYHAIILRALAMLYRILPQEPPLRKWVRSAIERAEAVLLD
                     ELYTSGASDADHSLTALCEVEKALGSSARRLGAIRVIVNAMIARLVDGRATELDDVSL
                     LAVGQAVEAFAKRS"
     gene            4295..5569
                     /locus_tag="P5316_17320"
     CDS             4295..5569
                     /locus_tag="P5316_17320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR86774.1"
                     /translation="MASHSGCKGDVPVFVQTGQTDAGGELDRWRQVGEEEARQAYQMV
                     LRNELSGGTPVVREFEQVWREMTGLKYCLTTCNGTLAIYSALFGLGVGPGDEVICPTY
                     TWICSISPALLLGARPVFCDSDPLTMQLDPADVRRRITPRTRAIVVVHLWGWVADMDA
                     IMAVSRETGVPVLEDCSHAHGATYKGRPVGSIGHVGCWSLQGTKPVSGGEGGVLATNN
                     VEIFDRACLLGQVNRMAGMDLQTASYQKHQPLGLGMKTRAHPLGIGIALVQLRKLAGL
                     NERRRQYVETVEAALKDLPGVAPVPTSEGSVRGGYYAFPIHYRPDELGGRPAGELIEK
                     LKSKGIPAGGSTYQLLHRLPLFAQGFDIFTLGRGPLCEGWRGYREGDFPMAEQLHRDL
                     VFLPMLSDPAPGAVDRVIAALRDSVRGMANQE"
     assembly_gap    5907..6006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6046..6840
                     /locus_tag="P5316_17325"
     CDS             6046..6840
                     /locus_tag="P5316_17325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRR86775.1"
                     /translation="MLENCLSEVSVNSDSFKWPNGQQGAVSLRFDDSMDSHIQRAAPL
                     LEQHGLRGTFYICPAGPDDQWRARAAEWKPVLEAGHEIGNHTMNHPIPAALADRPGPN
                     CYENLTLEKYAADVLAAHNRLENAFGPRDWTFAYPCYQTWVGRGRQCRSVVPFIAEHF
                     LAAAAGGEISKPFNDPRYCDLHVLLSLRADNLPIADLIRRVEQAVSMGRWAILTFHGI
                     QEGHLPVSAPVLDGLAAYLATNRERIWTAPVAEVARHTYDAGRDHQ"
     gene            7114..8364
                     /locus_tag="P5316_17330"
     CDS             7114..8364
                     /locus_tag="P5316_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86776.1"
                     /translation="MANPSTIVSKNIGQILNSALDAGQGVLRLTPTWVPRSFLHPGRR
                     IKLHPDDYYALGADRGGIDERWFASTTEAMNENRLPDEGLSYISFEGKRFLMKEAVAE
                     AGVRMIGKAMWDKYKRWPVYSKFFDNLGPIPHHMHQKHEHAKLTGQEGKPEAYYFSPQ
                     MNNCDNHFPYTFMGLEPGTTKAQVRRCLENWNKGDNGILYLSRAYRLMRGSGWLIPAG
                     VLHAPGSLCTYEPQWGSDVFGMFQNIVEGRFVPWDLLVKDVPKDKHHDLDYIVDQLDW
                     EKNVDTHFMEHNQLIPIVDKACSGPGYTDKWIVYGTIDGQQVVSAKELTIEPGAKCTL
                     KDPGASGWITVQGCGRIGKMRLQTPAMIRFGEETEDEVFITHEAATRGIEIENTGNEP
                     LVGLRYFGPDVHANLPKIGDHKKQ"
     gene            8477..8893
                     /locus_tag="P5316_17335"
     CDS             8477..8893
                     /locus_tag="P5316_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRR86777.1"
                     /translation="MTKQTRSMHGKSGRPEKTYDLEERTARFGEAIIAFARMIPVDPV
                     TQPLVGQLVRAGTSVGSNYCEADEAGSAKEFRYRISVCKRESKESKYWLRVIAVALPA
                     LKDKARALWLEAQELTLIFGAIHRSRKARQPAAGEA"
     gene            9000..>9421
                     /locus_tag="P5316_17340"
     CDS             9000..>9421
                     /locus_tag="P5316_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRR86778.1"
                     /translation="MSAAHINQYPKLHNAAWPGVVGKGPGAEEPAIDLDTMLDLTAAA
                     KADNAKFDGVDIFLFAPHVDIDASDDDLKRIAEKVTSRGLVIGSVVAPVWPPTGGGSA
                     MGDANDRKKFCEQVRKGCRIAEFWREMGVRPYGVVRIDS"
BASE COUNT         1789 a         2832 c         2979 g         1721 t
ORIGIN      
        1 ggtggcttgg tgatggctgc ataattctcg atggtcatct tgaaggagtt gtcgaaagga
       61 atgctgtccg cgacgtgcca gcggtaacag gactgccggc gacgctggcc gaactcgccg
      121 ggggtgggac agccgtggaa ggcgttggca aaatggcgaa tgccccacgc atcgccaaag
      181 tagtcctcgc tgccggtgcc gaaggttgac ggaaacttct cgccgtccac ccagactttc
      241 tcatctcctt cgccccacca gccgccggca atgttatcga tgaagagagc cgcgcccacg
      301 aatcgtcctg gaccggcgca ttccaggaag gggtagtcaa agtctttgct gaccggatcg
      361 cggcgccatt tggcgtggaa acgagcggct ccggccggcg gcccaccctg ctggtaaacg
      421 atccgggtgc ggagttgggc gggtctgcgg ccttggttgg tgaccacaaa acgcgccgat
      481 tgggcgaagg gcatacgcca atagcagtag ttcaggtcgt cggtgatgcc cagcggcaaa
      541 ctcttgtatg acgcctcgtc ccaggcatct ccgaaaaagt cgccgatcgg cgcttccacc
      601 gccggcgccg catcgccgtc gaagaagatt tgcagcaaga cctttcgacg ggcccagcgc
      661 tcttcagagg tcagcttggt caggaactga cgcacggtcc ccgggccttt cagatcggcc
      721 accggtatgg gctggccggg aaccaaggtg gtgttctttt cgatggtgag cgcgttctga
      781 accggttgag gatcaacgcc acagtttgcc agcgccttgc acaccctctg gaagacgatc
      841 tcctcggccg gcgtgtgctc caggctgaag gtcttgaccc gccattcctt cggaaacgtc
      901 gtatatccga tatggtagta ctggttgacg ggcttgtcgg cggttactct gcaactcttg
      961 gcgtagggaa tgggaacgta gcagtttgac gccgggttat caccccccac gtcggtgcgt
     1021 cgctgccaca caagcggctt ggggaaaccg gtaaccttgc cggtgaatag tgcgctgaat
     1081 tcatgctcaa gctggggctt ctccgccccg tcaaagtaga agcgaatccg cccctgcggg
     1141 ttcgccgacc agatccgcca gatacaaccc gggccttcca tctcggccat gactccctcg
     1201 ccggtggccg ggtcgatgcg aaggtagtgg ccggcatcgc cgttggcatc ccatttcaca
     1261 tactggtcgg aggcctcgtc gtacctggat gcccggtcat agctggagaa ctgcttgcag
     1321 gtctgcccgt gctccagaac gacgagatga tccagatcga tcagcttgtt cagaaaccag
     1381 gtgtagtctt tgagtgcgtc atccgtggct tgtgcccccg caaaagacac gctccaggcc
     1441 gtgaccgcca caagaatcag atgtgatctt cgcatgtttg tgcgtctcct ccgttctgtc
     1501 agagcttatg ggtcattctc gcgtggatgc tttccgacgc gcgtctgaaa gaacgatccg
     1561 tagtgtacct tcactgccgc gctgcacaag gggtgcgtga cagtctgggc gggcatgccg
     1621 accggtccag gttgggcgat caaccccgtt cagggggggc cgccaaaggc cggccgtgat
     1681 gcggccggtt ggtggtcgcc cgcggttctt cttcctttct gtggtctcag cccgtttcaa
     1741 cgggcttacc ggacaaggac aggcccgtta aaacggcctc gatgggataa cacagtaagg
     1801 gggggaattg gccgaccggg aaccagtcgt aaaacgactg gcctatttgc ccttcagtaa
     1861 agaccgctga agcgctctgc tttcggcttg cgctcaccac aaccgggtca gccgccagga
     1921 gtgccgccgc ttctgtcacc ggcccctgca caagcccctc atcgcaggcc ccttgccctg
     1981 gcccgtgcca cgtgctatac tcaaggtgtt cgtgcgagct ccatttcgca taccccaccc
     2041 ttcggcggag tggacaatgc ggactcattc tggccatcgt ggtttcacgc tgatcgagat
     2101 gctcgtcgtg gtcgcgatca tcgccctgct ggtcgctatt ctcctgcccg cgctggaggg
     2161 agcgcgccgg aacgccaaat cggtggtgtg cttgtccaac ctgatgagca tcggctcggc
     2221 gatgaagctt taccagggcg actaccacgg ctggcttccc gtcggaccgg cagacaagtt
     2281 gtggtacatc gacagcacgc tcgggacggt gcgcgagccc gctcccggca gaagaccgtt
     2341 cccttggtcg aactgtcatt ggggcggaaa gcgggcggct tggatccatg acgttgagaa
     2401 cgatccgccc aagccggagg ttctcggtcg tccgttgacc aggtacttgt accgcggaac
     2461 ggggctggac cagccgacac cgctgttcga gtgtcccgcc gacgtgggcg accccatgat
     2521 tgagaatccg gtgggccagt tgcctgttca ctatctttgt ggcaacagct actacacgaa
     2581 cccgtggaat cgctacaagc cccccggccg gcgacgccct tccccatcat cgatcgtgct
     2641 tgtcgaagat gctcctatgt acatggatct tgttcacaag cagcagcgga tgggctggca
     2701 cggtcggttc tccgtgcaca acgcgctgtt cctcgatttc catgccgaag ccaagtacat
     2761 ggatacgcga acgttcgtag gtcccggctg gttcgtcgag aactacttca acatcatgga
     2821 ttactacaaa tactgacgac agccggccga aagccgaagc ccgtcccatg cccagtcttt
     2881 ccctcgcctg cgttcggctg gttcgcgcct tgttggttgt gcattctggg cgcgggggta
     2941 tcatcacctg acgccggtct gggcatttca tgtgtcaaag ggcgccggcc ggaacatcgt
     3001 ctgttgcggt tcgtggatgc ggagcaatct tatgacaaca actcgccccg cagcaggtgt
     3061 gcctgagcct ccagaggttc gcgagcaggt cgatgcggct cgacgcggct tggcgaaggc
     3121 aatgctccaa caagacgcca atgctgttga atccgccgtt gcccgaatgc acgaggctct
     3181 gggcgagtgg gcgaccgtcc ccgagacggc tccgcggttt gccggttccc ccaatcctga
     3241 cgtcgctgtc gcgccggatc ggttacgacg tttgtggacc gccggttggg ctgccgcgga
     3301 caaggcatac agcggagatg gccgctgggg cttgccaagg gatcatcccg aggtgcgcca
     3361 cacgttgctg cgtcagacgg cctacgtcat catcggcggg atcgccgcga tgcggcaggg
     3421 cttcggcgac gaggcggcaa tccgcaaacg agtaaaggaa ggtctcgatt acctgctttc
     3481 agtgcaaaaa ccgaatggct tgttcccttt tcccgatgtg cgcggctggc atccgcactt
     3541 ctcgccgccg ctgcagtcgc tttacgctca acaccccgag gcgttccagg acggctggat
     3601 catcgacgat ttcggcgacg gcggcctgca gttcgacaac ggtgtgtgcg cggtgacgat
     3661 gttgacggca ttcgagcact tcaagacgac ggcgtaccgc gaatccgccg agcgggcttg
     3721 ccgctgggcg gtcgaacggc cggtcgtgcc gaattggaac tacaacgcct tttcggtctg
     3781 ggccttggcc cggtgcgttg aagctgtcgg ccgtgaggac tttcgtgccc cggccataca
     3841 gaaaacggtg cttggcattc tgccggggca actgccggcc ggccgctggc tcgaccgcca
     3901 taactgccga accgtgtatc acgcgattat tctgcgggcc ctggccatgc tgtatcgaat
     3961 tctgccccag gagccgccgt tgcgaaaatg ggtgcgaagt gccatcgagc gggctgaggc
     4021 ggttctgctc gacgaactgt acacctccgg ggcctccgat gccgatcact cgctgaccgc
     4081 gttgtgcgag gtcgagaagg cccttggctc gtcggcacgg cgcctgggcg cgatccgcgt
     4141 gatcgtcaat gcgatgatcg cgcggctggt tgatggtcga gcgacggaac tggacgatgt
     4201 cagcttgttg gcggtgggcc aggcggttga ggctttcgca aaacgatcgt aggctgaagg
     4261 cggaatgatg aatggtgaac ggcgagcaat gaacatggcc tcacactccg ggtgcaaagg
     4321 cgacgttccg gtcttcgttc agaccggtca aaccgacgcc ggcggcgagt tggatcgctg
     4381 gcgccaggtt ggcgaggaag aagcacggca agcttaccag atggtgctgc gcaatgaact
     4441 gtcggggggg acgccggtgg tgcgcgagtt cgagcaggtg tggcgcgaga tgaccggcct
     4501 gaaatactgc ctgaccacct gcaacggcac actcgcgatc tacagcgcgt tgttcgggct
     4561 gggcgttggg ccgggcgacg aggtgatctg cccgacgtac acgtggatct gctcgatcag
     4621 cccggcactg cttctgggag cacggccggt tttttgcgac tcggacccgt tgaccatgca
     4681 actcgatccc gcggacgttc gccggagaat cacgccgcgc acgcgggcca ttgtcgtggt
     4741 tcatctgtgg ggctgggtcg cggacatgga cgccatcatg gccgtcagtc gcgagacggg
     4801 tgtgccggtg ctcgaggact gctcgcacgc tcacggagcc acgtacaagg gacggccggt
     4861 cgggtcgatc gggcacgtcg gctgttggag cctgcagggc accaagccgg tcagcggcgg
     4921 cgaaggcggc gtgctcgcga ccaacaacgt cgagatcttt gatcgcgcat gcctgctggg
     4981 gcaggtcaac cgcatggccg ggatggacct gcagaccgct tcgtatcaga agcatcagcc
     5041 gctcggtctg ggaatgaaaa cgcgggcaca tccgctgggc atcggcatcg ccctggtgca
     5101 gttgcgcaag ctggccgggt tgaatgagcg gcggcggcag tatgtcgaaa cggtcgaggc
     5161 ggcgttgaag gacttgccgg gcgtggcccc ggtaccgacg tccgagggtt ccgtgcgcgg
     5221 cgggtactac gcgttcccga tccactatcg gccggacgag ctcggcggcc ggcccgccgg
     5281 agagctgatc gagaagctga agtcgaaagg cataccagcc ggcgggagca cctaccaact
     5341 gctccaccgg ctgccgctgt tcgcgcaggg cttcgatatc ttcacccttg gccgcggacc
     5401 gttatgcgaa ggctggcgag gctaccgcga gggcgacttc cctatggccg agcaactgca
     5461 ccgcgacttg gtgttcctgc cgatgctgag cgacccggca ccaggggcgg tcgatcgggt
     5521 cattgcggcc ctgcgggata gcgtgcgagg aatggcgaat caggaatgaa gacatgagaa
     5581 ttgcccgagt gaggtgagcg tgagcagctc ggcccggaat ccctgatacg cttcccgagg
     5641 ttagtcatag gcgtgcgcag cgatcccgga gccgcaagcg gagcacgcct tccggaccgt
     5701 ttgtcttcgc ggaatccttt ccgcatcgtc accagtgagg atgggaatcc tcaagcgggg
     5761 aggccccaga acggattctg gggccagcca taatggcgaa tcaggcatga atgcttgaga
     5821 attgcccgct cggcccggaa tcccttccgg gccgtttgtc ttcgcggaat cctttccgca
     5881 tcgtcaccag tgaggatggg aatcctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnncgga ttctggggcc agccataatg gcgaatcagg catgaatgct tgagaattgc
     6061 ctgagcgagg tgagcgtgaa cagcgattcg ttcaaatggc cgaatgggca gcagggggcg
     6121 gtgagcctgc gattcgatga cagcatggat tcgcacatcc agcgtgccgc tccgttgctg
     6181 gagcagcacg ggctgcgggg cacgttctac atctgcccgg ccgggccgga cgatcagtgg
     6241 cgggcccggg ccgccgagtg gaaaccggtc ctggaggcag gccacgagat cggcaatcac
     6301 acgatgaacc atccgattcc ggcggcgctg gccgaccggc cgggaccgaa ctgctacgag
     6361 aacctcacct tggaaaagta tgccgccgat gtgctggcag ctcataaccg gctggagaac
     6421 gccttcgggc cgcgggactg gaccttcgcc tacccgtgct atcagacctg ggtcggccgg
     6481 gggcggcagt gccgcagcgt tgtccccttc atcgccgagc acttcctcgc ggcggcggcc
     6541 ggcggcgaga tctccaagcc gttcaacgat ccgcgatact gcgatcttca cgtgctcctc
     6601 agcctgcggg ccgacaacct gccgatcgcc gatctcatcc gccgcgtcga gcaggccgtc
     6661 tcgatggggc gatgggccat cctcaccttc cacggcatcc aggaggggca tctgccggta
     6721 agcgccccgg tgctggatgg actcgcggca tacctggcga cgaaccgcga acgcatctgg
     6781 acggcaccgg tggccgaggt ggcgaggcac acgtacgacg ccgggcgcga tcatcaataa
     6841 ggctttctga ccccaaccag tttgacgagc cggcagggtt ctcctgccag cccttctgca
     6901 ttccccgaag acccaggggg caagcccctc ggctcgccag gaaaacgacc ggaccgtcct
     6961 tcttcgcttc gttccgctcc agcccccagc ccccagtccc cgatccccag cccccgtttt
     7021 caccttgcct gccccggcgg ggtcgggtaa cttggctggc ccggtcgagg cgaggggccc
     7081 tggccgtagg taccagtcgg agagatcacg atcatggcga acccaagcac gattgtcagc
     7141 aagaacatcg gtcaaatctt aaacagcgca ttggatgccg gtcagggcgt cttgcggctg
     7201 acccccacgt gggtcccacg aagcttcctg caccccggac ggcggatcaa actgcatccg
     7261 gatgactact atgccctggg ggccgatcgc gggggaatcg acgaacggtg gttcgccagc
     7321 acgaccgagg cgatgaatga gaaccggctg cccgacgagg ggctcagcta tatctccttt
     7381 gagggcaagc gcttcctgat gaaggaagcc gtggccgaag ccggtgtcag gatgatcggc
     7441 aaggccatgt gggacaagta caaaaggtgg ccagtctact cgaagttctt cgataacctc
     7501 ggccccatcc cacaccacat gcaccagaag cacgagcacg ccaagctcac cggccaggag
     7561 ggtaagcccg aggcctacta cttctcgccc cagatgaaca actgcgacaa ccacttcccg
     7621 tacacgttca tgggcctcga accgggcacg accaaggccc aggtccgccg gtgcctggaa
     7681 aactggaaca agggcgacaa cggcatcttg tacctctcca gggcctaccg gctcatgcgc
     7741 ggctcgggct ggctgattcc ggccggcgtg ctgcacgctc caggttccct gtgcacctac
     7801 gagccccagt ggggttcgga tgtgttcgga atgttccaga acatcgtcga agggcgattc
     7861 gtgccctggg atctgctggt caaggacgtg cccaaggaca agcaccacga cttggactat
     7921 atcgtcgacc agctcgactg ggaaaagaac gtcgatacgc acttcatgga acataaccag
     7981 ctcatcccga tcgtggataa ggcttgcagc ggccccggtt acaccgacaa gtggatcgta
     8041 tacggcacca tcgacggcca gcaggtggtc agcgccaagg aactgaccat cgagccgggt
     8101 gccaagtgca cgctcaagga cccgggcgcc agcggctgga tcaccgtcca gggttgcggg
     8161 cggatcggca agatgagact tcaaacgccg gccatgatcc ggttcggcga agaaactgaa
     8221 gacgaggtat tcatcaccca cgaagccgcc acccggggaa tcgagatcga aaacaccggc
     8281 aacgagccgc tggtcgggct gcgatacttc ggacccgatg tacacgccaa tctgccaaag
     8341 atcggcgacc ataaaaagca atgaccaatg accaaaaccc aatgaccaat gaatgacgaa
     8401 gcccgaatcc cgaatagaag ccgaaccagg taccgccggc agttaaggag acggagacct
     8461 cgaaagcacc gcgccaatga ccaaacagac ccgaagcatg cacggaaaaa gcggcagacc
     8521 ggagaaaacc tacgacctgg aggagcgcac ggcgcgattc ggcgaggcga tcattgcgtt
     8581 tgccaggatg attccggttg accccgtgac gcagccgcta gtcggacagt tggtcagggc
     8641 cggtacaagc gtgggctcca actactgtga ggccgatgag gctggttcgg cgaaggagtt
     8701 tcgataccgg atcagcgtct gtaaaagaga gtcgaaagag agcaaatact ggctgcgagt
     8761 gatcgctgtt gctctgcccg cgctcaaaga caaggctcgg gcactctggc ttgaggccca
     8821 ggaactcacc ctgatcttcg gtgccatcca tcgaagcagg aaggcccgac aaccggcagc
     8881 cggcgaagcg tagtctaggc tgagaggtcg tcgcaacgag gcggttcgtc attcgggctt
     8941 ggtcattgat tggtcattgg ggcttcggca ttggtcatta accgtaggga ggaacgctcg
     9001 tgagcgctgc acacatcaat cagtatccta agcttcacaa cgccgcctgg ccgggcgtcg
     9061 tcggcaaggg ccccggggcc gaggagccgg ccatcgacct ggacaccatg ttggacctca
     9121 cggccgccgc gaaggcggac aacgccaagt tcgacggcgt ggacatcttc ctgtttgctc
     9181 cgcacgtgga catcgatgca tccgacgacg atctcaaacg catcgccgag aaggtgacgt
     9241 cgcgcgggct ggtcatcggc tcggtggtcg caccggtctg gccgccgacc ggtggcggct
     9301 ccgccatggg cgacgccaac gaccgcaaga agttctgcga gcaggtgcga aaaggatgcc
     9361 ggattgctga gttctggcgc gaaatgggcg tacggcccta tggtgtggtg cggatcgact
     9421 c
//
LOCUS       DAOTPW010000228         9320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6780_length_9320_cov_5.424825, whole genome shotgun sequence.
ACCESSION   DAOTPW010000228 DAOTPW010000000
VERSION     DAOTPW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6780_length_9320_cov_5.424825"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(702..2369)
                     /locus_tag="P5316_17345"
     CDS             complement(702..2369)
                     /locus_tag="P5316_17345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR86779.1"
                     /translation="MRGDQEVSCGLSARQVFLMRVLLIQPPVDLFDHDEKQSMPPLGL
                     AYIAAVLERAGHEVAILDCVVAAFQRLRRLQDGRLRHGLDAEGIVKEVCRFKPSLVGV
                     SNLFSAQSQAAHFVCRLVKETDPAIVTVMGGAHPSAVPNEVLGDRHVDYVIQGEGELA
                     MLRLADALQRGRRIPDDPSGLAFREDDTIRVCPPGPRLVDLDSLPLPARRLLPMQHYF
                     RYRAPHGTVVRRHPCTNMITSRGCPARCSFCSIHTVWGRRFRWHSARRVVQEIDVLVH
                     DYGVREIQFEDDNLTLNKPRMREICRRIIERRFDLFWTTPNGVATWSLDEELLALMRH
                     AGCYHVAIAVESGVQDTLDRLIRKPLDLRKVPPLIRAARALGMGVSAFFVVGFPGETR
                     ADIEKTFDYAMNLGADHVHFFTATPYPGTDLFRQCVETGLLKAPVDYAQLRVGHPMFD
                     TAEWSADELVAMTRAAQSRFYRQTAVRRPVRFAATVARKFAREPRLTTRRAWNVLFGR
                     QGIDIRSGGPAAEATPLSSRTRLGEVMEETPSMPEGVAPGGTRSLNP"
     gene            complement(3104..4249)
                     /gene="hemW"
                     /locus_tag="P5316_17350"
     CDS             complement(3104..4249)
                     /gene="hemW"
                     /locus_tag="P5316_17350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRR86780.1"
                     /translation="MTDVGLYVHVPFCPRKCGYCDFYSITPDDSLVAGYIDAVLSELD
                     AVVAEDRVRVETIFVGGGTPTILPESSLARLMEHLAQIYRVHRVVEFSVEANPASLTE
                     EKAFLLREAGVNRVSIGAQSFVPAELEILERLHRPEDIAAGVELARRAGFEHLNLDLI
                     FGVPGQTLDTWNRSLGAAIDLGPDHVACYGLTYEPGTPLAVRRDCGQITPVDEEVETE
                     MYMRAIDRMAEAGFQQYEISNFARPGAECQHNLRYWRNRPGIGVGPAAASYLNGCRWR
                     NVVSVEEYIARVRAGRSTAVDVECLTPVERAGEIAMLRLRTNEGILCEEFRAETGYDA
                     QELFAEAIRSHCRAKLLTADDDRIALTRKGLLVADAVIRDFLKPSGC"
     gene            complement(4266..6020)
                     /locus_tag="P5316_17355"
     CDS             complement(4266..6020)
                     /locus_tag="P5316_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRR86781.1"
                     /translation="MNAHRKLDRLVVLGLLVLGGCGEDFWGSKKPTTRPSGTLPDLDA
                     MRRSVQSVAMEGTIGSVANLQGMRYMRVRGYGLVWGLNGQGSRFCPPAIRERILRDIR
                     RFRLANPHLERNLTAEELIDSMNTAVVEVTGDVPAGAQEGTVFDVVVSAVATSPDARS
                     LAGGHLLPCELQILQETSPGDAIEGRTHAKARGPVFTNPFIGSSTQPAADTSLLEGRV
                     IGGGVNTLKRRLGLDTFVPSYRTVREIADAVNRRFVGDPKIADPISSSHVDLRVPDEY
                     RGREARFIELVMHLPLSPSPAVREMRTKALISALSSSIEPPEDAALSLEGIGPSVITM
                     LSPLYTHPQRQVSWYAARTGLRLGDAPALEVVARHARDPKSPYRMQAIREMGSCRRMA
                     LATTVLRELLDSPDTWVRIRAYEALRTADRSAMVSLVVGREPGNFILDVLPSAASPLI
                     YARRSEVRRIALIGADQMSFRLPLFYSKPGKQVTLSAAAGDQKLTVLRKELNKVAGEF
                     KVPLSVVQMTRFMGEDPRMGPDKTPAGLGLDYAAVLDVLYTLCQDGALNARMEWEQQS
                     IEDLIGPLRPRGRPESDL"
     gene            6257..6412
                     /locus_tag="P5316_17360"
                     /pseudo
     CDS             6257..6412
                     /locus_tag="P5316_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246889.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
     gene            7009..7923
                     /locus_tag="P5316_17365"
     CDS             7009..7923
                     /locus_tag="P5316_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HRR86782.1"
                     /translation="MSISSILLDGMRGVIDCVEAARHSPLPSPVPAAECTRLLEEYRR
                     TRAITSKLANRLVGKIPKNTLDACARTLGMMVRGTLVFDDINETDVLMDYCIFFGLDQ
                     GATVISRFAATSPYPEGSAETLALQAMCNSKYRFLAVTAVAPSLGIEAVDLLRNERRL
                     IADESFSRMAVPGLLLASRIVDMPEFSMTTGAALPVTVEVLEEIWHQLDADRGNINSI
                     DPKNATFEQEVALATLIISAALKHRTSCRIRYVEPGKDPGEWDEGHDLSPSRPGRLSP
                     GHRVSRNDPCPCGSNLKYKNCCGRRRYI"
     gene            complement(7973..8857)
                     /gene="mtnP"
                     /locus_tag="P5316_17370"
     CDS             complement(7973..8857)
                     /gene="mtnP"
                     /locus_tag="P5316_17370"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRR86783.1"
                     /translation="MADARIGIIGGSGLGQVLAEQVGGKQVQVETPFGNPSGPILLTR
                     WAGSDIAFLSRHGPGHLLNPSNVPYRANIYALKSLGVTHIVASGATGSLREDIEPRHL
                     VICDQVIDRTFRRESTFFDRGIVAHVEFAEPFCPRLRALLRSVGEKIGARVHSAGTYV
                     CMEGPQFSTVAESRMYQQCGGDLIGMTAMPEAKLAREAEICYALIALPTDYDCWRPHE
                     AGKDREALLREIIGNLNAVTTNAIELLRAAMPALAGQIDAPCACREALAMAIWSDRSR
                     IASADVERLSLLVGRYFT"
     gene            complement(9174..>9320)
                     /locus_tag="P5316_17375"
     CDS             complement(9174..>9320)
                     /locus_tag="P5316_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR86784.1"
                     /translation="RKQYRQRNLAERFVNRIKRFRRVATRYEKTARNFMSFVQLAALF
                     CWMT"
BASE COUNT         1951 a         3090 c         2634 g         1645 t
ORIGIN      
        1 caagcgcaac cgcaattcgc cgccgctccg ctccaaccgc cccctccgga gcaccccccg
       61 tgttctccct cccgacaaca ccggcggaga ccctgcgcgc caaccgcgca ggatcacggc
      121 cttcaactaa actgatcccg cagccggcca ggggagaaac gccatgatct gtgcaacctc
      181 gccatccctc agcccatgta atccacaacc ttcgcacgtg aaacgcatta cgcgcagcgc
      241 gaccatggat acatcgccgg tcaccaaccg aatcctcttc gctcaccaat caaatcgcca
      301 aacaaaccca acattcattc gtgcttcaca ctccttcgtc catgcatcgt cattgctggt
      361 tgccccgttc gccgtccgct ggccggattc ctcgcgtcac gaacggtccg accatagtca
      421 tggcggaaca aacccaagcc ccgccgacct gagccgcagg gatgaccacg ccttgtcgaa
      481 aagagcctgc tagtagctca agctagacca atgtgttaat agaccgcgta gacaaggtaa
      541 aaatgtataa atattctaga cagggcatgc atttacagca gccccccagg gcctcgtgca
      601 agggtatccc cgcctccgcg gtttcctcgc tcccgtcgcg gctccactga ccgtccgagc
      661 acgtcgggcg tttcggcggc tcggtctgcc acccatgttc atcaaggatt caggctccgc
      721 gtcccgccgg gcgcgacgcc ctcaggcatc gaaggggtct cctccatcac ttcgccgagg
      781 cgagtccgcg atgacaacgg cgtcgcctcg gcggccggtc ccccgctgcg gatatcgatg
      841 ccttgcctcc caaaaagcac gttccaggcc ctgcgcgtgg tcagacgtgg ttcgcgggcg
      901 aacttccggg caacggtggc cgcgaatcgc accggccgtc gaacagcggt ttggcggtaa
      961 aacctggatt gggccgcgcg ggtcatcgcg accagctcgt cggcgctcca ctcggccgta
     1021 tcgaacatcg ggtgtcccac gcgaagctgc gcataatcca cgggcgcctt caacaaaccc
     1081 gtttccacgc attgccggaa cagatccgtt cccggatacg gcgtcgcagt gaagaaatga
     1141 acgtgatcgg cacccaggtt catcgcatag tcgaacgtct tctcgatatc ggcacgcgtc
     1201 tcgcccggaa aaccgacgac gaaaaacgcc gaaaccccca tgcccagcgc gcgggccgcc
     1261 cggatcagcg gcggaacctt gcgaaggtcc aacggcttcc gaatgagccg atcgagcgta
     1321 tcctgaacgc cagactccac ggcaatcgca acgtgataac agccggcatg tcgcatcagc
     1381 gccaacagct cctcgtccag cgaccacgtg gctacgccgt tcggcgtcgt ccagaacaaa
     1441 tcgaatcgcc gttcaatgat cctccggcag atctcgcgca tgcgcggttt gttgagcgtg
     1501 agattgtcgt cctcgaactg gatctcccgc acgccgtagt catgcaccag aacgtcgatc
     1561 tcctgcacga cgcggcgggc gctgtgccag cgaaatctgc gaccccacac ggtgtggatg
     1621 ctgcagaagc tgcaacgagc agggcaacct cgcgacgtga tcatgttggt gcacgggtgc
     1681 cgccgaacca ccgtgccgtg cggcgcccga taccggaagt agtgttgcat cggcaacaaa
     1741 cgacgcgccg gcaagggcag cgaatccaga tcgacaagcc gcggccccgg cggacagact
     1801 cgaatcgtgt cgtcttcgcg aaaagccaag cccgatgggt catccggaat acgacggccc
     1861 cgctgcaagg cgtctgccag acgcagcatc gccaactcgc cttcaccctg tataacgtag
     1921 tcaacgtgcc gatcgccgag cacctcattg ggcactgccg aaggatgagc cccgcccatc
     1981 accgtgacga ttgccgggtc cgtctctttg acaagacggc acacaaagtg cgcggcctgc
     2041 gactgggccg aaaagagatt cgacacgccg acaagggatg gtttgaacct gcagacttcc
     2101 ttgacgatcc cttctgcatc cagaccgtgt cgaagtcgtc cgtcctgcag ccgacgaaga
     2161 cgctggaacg cggccaccac acaatcaaga atggcaacct catggccggc ccgctccaga
     2221 acggccgcaa tgtaggccag tcccagcgga ggcatcgact gcttctcatc gtggtcgaag
     2281 aggtcgacgg gcggctggat cagtagaacg cgcatcagga acacctgccg agccgacagg
     2341 ccgcaactga cctcttgatc accgcgcacc cgcgctgacg tccacaacgc tgcgccgcac
     2401 cttgccccac catgggcata cggtcggctg ccctcggctc gacgaagtta tcggctattg
     2461 ctgggaaggt ctggagagtc aaaaggcccg ataacaagag cgggcaatcc cgcactgcga
     2521 gaagcccgct cgacattcag tcctgccaat cctttccctg cagtcgcttg cagggcggcg
     2581 aaaacactag cgcctgctgc ggttgccgag tttcggcaat tcggcgggtg cccgcggcgg
     2641 ctttgggcct tcgcagccga agcgtccgcc tcgacgcgga gtaacctgct cgccggtgca
     2701 caactgatcc gccgaagacg gcactagctc tgctgcttct tcttgatgat cagcagcgca
     2761 accacaagaa cggccagcac aacaataaga acgatattcc acgttgccat acccaagata
     2821 ttccacgctg ccatatgtat ctccttctgc tgatgtgtgc ccgcacatcg gggccactgg
     2881 cacaaaccgc aacacgtcgt cgcagaccaa ggcgcgacaa ctcaccggtg cgcgttggtg
     2941 caacattagg ccggaagctt caaagactgt caagtgggaa atgtcagaac gttcggcccg
     3001 taaccctccg accggacggc cagcctccgc tgtctcgata aactcacaga cgcctgccga
     3061 acctcccaca agcaggccgc ccggccatcg gtgtccacga cgctcagcag ccgctcggct
     3121 tgagaaagtc ccggatcacg gcatccgcca caagcagccc ctttcgggta agggcgatcc
     3181 ggtcatcgtc ggcagtgagc agcttggccc tgcaatgact ccggatggcc tcagcgaaca
     3241 actcttgcgc gtcatagccg gtttccgctc gaaattcctc gcaaagaata ccttcatttg
     3301 tgcgaagccg aagcatggca atttctccgg cccgctcaac gggggtcaga cactcgacat
     3361 ccaccgccgt actccgcccc gcccgaaccc tggctatgta ttcctccaca ctgacgacat
     3421 ttcgccaccg gcagccgttc aagtaactgg ctgcggccgg cccaactcca attccgggcc
     3481 ggttccgcca gtacctgaga ttgtgctgac actcggcgcc aggacgagcg aagttgctga
     3541 tctcgtactg ctggaaaccg gcctcggcca tgcggtcgat ggcccgcata tacatttcgg
     3601 tctcgacttc ctcatcgacg ggcgtaatct ggccgcaatc gcgtcggaca gccagaggcg
     3661 tgcctggctc ataggtcaat ccgtaacacg cgacatggtc aggccccaag tcgatggctg
     3721 ctcccagcga cctgttccac gtgtcaagcg tctgacccgg taccccgaaa atcaggtcca
     3781 gattgaggtg ctcgaaacct gcccgacgag ccagctcgac gccggcagcg atatcctccg
     3841 gacggtgcaa ccgttcgagg atctcaagct cggcaggcac aaacgattga gccccgatcg
     3901 acacgcggtt tacccccgcc tccctgagca ggaaagcctt ttcctctgtc aatgaagccg
     3961 gattggcttc cacggaaaac tcaacgacgc ggtggacccg gtatatctga gccagatgct
     4021 ccatcaagcg ggccaaagac gactcaggca ggatggtcgg tgtgccgccg ccgacgaaaa
     4081 tggtctccac acgcacgcgg tcttcagcga caacggcatc gagttcgctc aagaccgcat
     4141 cgatgtagcc ggcaaccagt gaatcgtccg gcgtaatcga atagaaatca caatacccgc
     4201 acttgcgagg gcaaaaggga acgtgaacgt aaagaccgac atcggtcatg ataatgcggc
     4261 gaagatcata aatcagattc ggggcgcccc ctcggccgta gggggccgat caggtcctcg
     4321 atgctctgct gttcccattc catgcgggcg ttcagggccc catcctgaca aagggtgtac
     4381 aacacgtcga gcaccgctgc ataatcgagg ccaagtcccg ccggcgtctt gtccggcccc
     4441 atcctcgggt cctcgcccat gaaacgagtc atctgcacga ccgagagcgg aaccttgaac
     4501 tcacccgcca ccttgttgag ttccttgcga aggacggtca gcttctgatc gcccgcggcg
     4561 gccgacaaag tcacctgctt gcccggcttg gagtaaaaca gcgggagccg aaaggacatc
     4621 tggtccgcgc caatcagtgc gatccgtcgg acctcgcttc gtctcgcgta gatcagggga
     4681 gacgccgccg agggcagcac gtcaaggatg aagttcccgg gctcacggcc gaccaccaag
     4741 gacaccatgg ctgaccggtc ggccgttcga agggcttcgt aagcccgaat gcggacccag
     4801 gtgtcggggc tgtccagaag ctctcgaaga accgtggttg ccaacgccat tcggcggcag
     4861 ctacccatct cccggatcgc ctgcatgcga taggggctct tggggtctct cgcgtggcgg
     4921 gcaaccacct ccaaggcggg ggcatcacca agtctcaaac cggttctggc tgcgtaccag
     4981 ctcacttggc gttggggatg cgtgtagagc ggcgaaagca tggtaatcac cgaaggcccg
     5041 attccctcta atgacaaagc cgcgtcctcc ggcggttcga tcgaactcga caacgcgctg
     5101 atcagcgcct tggtacgcat ctctctcacc gcaggtgacg gcgacaaggg caagtgcatc
     5161 accaactcga taaaccgagc ttcccttccg cgatactcat ccggtactct cagatcgacg
     5221 tggctggaag atatgggatc cgcgatcttc gggtcaccga caaaacgacg attgaccgca
     5281 tcggcaatct cgcgaaccgt ccgataggac gggacaaatg tgtcgaggcc gagtcggcgc
     5341 ttcagagtgt tcacgccgcc gccgatcact ctgccctcca aaaggctggt gtcggccgca
     5401 ggctgcgtgc tcgagccgat aaagggattc gtgaaaaccg gcccgcgagc tttggcatga
     5461 gtccgcccct cgatcgcatc gcctggcgag gtttcctgaa ggatctggag ctcacagggc
     5521 aggaggtgcc cgccggccag cgaccgcgcg tccgggctgg ttgccaccgc cgacacaacc
     5581 acgtcgaata cggtcccctc ctgcgcgccg gccggcacgt caccggtgac ctccacgacc
     5641 gccgtattca tgctgtcgat cagttcctcc gccgtcagat tcctctccag atgcgggttt
     5701 gccagccgga accggcggat gtctcgcagg atccgctcgc gtatcgccgg aggacagaac
     5761 ctgctaccct gcccgttcag tccccacacc agcccgtagc ctcgcacccg catgtagcgc
     5821 atcccctgca ggttggccac cgagccgatt gtcccctcca tcgcgacaga ctgaacagaa
     5881 cgtcgcatgg cgtccaggtc gggcagcgtg cccgacgggc gggtagtcgg cttcttgctg
     5941 ccccagaaat cctcgccgca cccaccaagc acgagcagac ccaagaccac gagccggtcg
     6001 agtttgcgat gcgcgttcat ccgaggacct ccgactgctg cgaagaaaag caacaaggca
     6061 ggcaacggaa ccggcccttc tgcaagggcc gcccctcgcg gcaataacag ggcgtgtggg
     6121 aaccatcccg accatccacg gccatgtgct aacgtagcca tacggaaagg cgatgtcaac
     6181 gcaatacgga ctggcggcgg cgacccaaca cggtacaatt ggcttccggt cgtcgagaag
     6241 accccgagga actgcgatgc gaacatgtcg ccggccggcg ttcaccctga tcgaagtgct
     6301 ggttgtgctt gccatcatcg ccttgttggt ggcgatcctg ctgcccgccg tgtctcgcgt
     6361 ccgcgaccag gcccgccttg ccatctgcgg cagcaatctg cgacaaatcg gcacgggcat
     6421 caccgcctat gccaacgctt gccgcgtcat tccgcacggt ccggtcgtcc agagtctggg
     6481 cggaatcctg gagtccaatg acggcaccgt ggccaccagc cagatctgga ccggaccgca
     6541 agagccgatc cgctccagga tggcgctggg attgctcctc tgtcagcaaa acatcacgtc
     6601 cgaggttctc tattgtccag gagatgattc gaacgacccc gtggaggagc tggccaagat
     6661 cctgatgcgg aaaatggctc cggggttctg ctccttcctc taccggcagt tggatgagac
     6721 cgacggtcgc ggacaactcg acagactcgg actgaacggc atcgggcaac gggcgacggc
     6781 cttggccctg gacatgaact cgctcatcac catcgacccg tcatacacgc gaaccaatca
     6841 cagggcccgg caactcaaca tcctcagctt cgacggctcg gtaagacagg tgagaaacat
     6901 cgataatcgg tttgccattc gaaatgaaga tctggtcaga tcgacctacc gacggaaaga
     6961 catccttcgc gaggccgaca gggtttacta gccacggaga caccggaaat gtccatcagc
     7021 agcattctgc ttgacggaat gaggggcgtg atcgattgcg tggaggctgc ccgccacagc
     7081 cctttacctt ctccagttcc cgccgccgag tgcacgcggc ttctggaaga gtatcgccgc
     7141 acccgcgcga tcacctccaa actggccaac cggttggttg gtaagatacc caagaacacg
     7201 ctcgacgcgt gcgcaagaac gctgggcatg atggtgcgag gcacgttggt gttcgacgac
     7261 attaacgaaa cggacgtcct gatggattac tgcatcttct tcgggctcga ccagggcgct
     7321 acggtgatct cacgtttcgc cgcaacctca ccgtatcccg aagggtcggc ggagacgctg
     7381 gcactccaag ccatgtgcaa cagcaaatac cgttttttgg cagtgaccgc cgttgccccc
     7441 tccctcggca tcgaggccgt cgatttgctg cgaaacgaac ggcggctcat tgcggacgag
     7501 tcgttcagtc gcatggcggt tcccggtctc ctgctggcga gccgaatcgt cgacatgccg
     7561 gagttttcaa tgaccaccgg cgccgctctg cccgttactg ttgaagtcct cgaagagatc
     7621 tggcatcagc tcgacgccga tcgcggcaat atcaactcca tcgatccgaa aaacgcaaca
     7681 ttcgaacagg aggtcgcctt ggcgaccttg atcatttcag cggcgctcaa gcaccgtaca
     7741 tcctgccgga ttcggtatgt cgagcccggg aaagaccccg gagaatggga cgaaggtcac
     7801 gacttatccc cgtctcgccc gggcaggctg tcgcccggtc atcgcgtctc gcggaacgat
     7861 ccatgtccgt gcgggagcaa cctcaaatac aagaattgct gcgggcgacg gagatacatt
     7921 tgacgatcca tgggcctatt cacctgatgg gtaccgcagc caccgccccc aatcacgtga
     7981 agtaccggcc aacgagcagg ctgagtcgct ccacgtccgc ggaggcgata cgcgagcggt
     8041 cagaccagat ggccatcgcc aaggcctcgc ggcaggcaca aggggcatcg atctgccccg
     8101 ccagcgccgg cattgccgcc cgcaaaagct cgatcgcgtt ggtggtgacg gcgttgagat
     8161 tgccaatgat ctctcgcagc agggcctcac gatctttgcc cgcttcgtga ggccgccagc
     8221 aatcgtagtc agtgggcaag gcgatcaggg cgtaacagat ttcggcctcg cgggcaagct
     8281 tggcttcggg catcgcggtc atgccgatca ggtcgccgcc gcactgctga tacatacgcg
     8341 actcggccac cgtcgaaaac tgcggtcctt ccatgcatac gtaggttccc gccgagtgga
     8401 cgcgagcgcc gatcttctcg ccgaccgacc tgagcaaagc tcgcaacctg gggcagaaag
     8461 gctccgcaaa ctcaacatgc gccacgatac cgcgatcgaa aaaggtgctt tctcgacgga
     8521 aggtccggtc gatgacctga tcgcagatga ccagatggcg gggctcgatg tcctctcgca
     8581 ggctgccggt cgccccgctg gccacgatgt gcgtgactcc caacgacttc agagcgtaga
     8641 tgttcgctcg atacggcaca tttgagggat tcagcaggtg gccgggtccg tgacgtgaga
     8701 gaaaagcaat atctgaaccc gcccatcgcg tcaggaggat cgggccgctc ggattcccga
     8761 acggcgtctc cacctgcacc tgcttgccgc caacctgctc ggccaagacc tgcccgagtc
     8821 ccgaaccgcc gatgatcccg attcttgcgt cagccacgtc gtactcccgc tcaagagctt
     8881 catggtcaac agccgcccgc ccctccggca caacagcgtg gtgattatac cggggcgggc
     8941 gcacttttcg gacaatgtcc gaacaccgaa ccgcgaggca caacgcccca aagggcggat
     9001 accatccccg gcctccggcc cgctctcaga cccatcggct ggccccacgt gcgtccgccg
     9061 catagaatgg ccaccaattg cacgcaaagg ttcccaggcg agaggtgcat tgtgatcgaa
     9121 caagtcttct caattgcctc ctggccgatg gccgtattag gtcgtgttga cattcaggtc
     9181 atccaacaaa agagggcggc gagttggacg aagctcatga agttccgggc ggttttctcg
     9241 tagcgggtgg cgacgcgacg aaaacgcttg atccgattca caaatcgctc cgccaggttg
     9301 cgttgccggt actgcttcct
//
LOCUS       DAOTPW010000229         9273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6809_length_9273_cov_4.804947, whole genome shotgun sequence.
ACCESSION   DAOTPW010000229 DAOTPW010000000
VERSION     DAOTPW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6809_length_9273_cov_4.804947"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2188)
                     /gene="rpoC"
                     /locus_tag="P5316_17380"
     CDS             complement(<1..2188)
                     /gene="rpoC"
                     /locus_tag="P5316_17380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRR86785.1"
                     /translation="MAENIYDRINDYGSVKISLASPNDIRSWSFGEVKKPETINYRTY
                     RPEKDGLFCERIFGPERDWECACGKYKGTKHKGIICDRCGVKVTHSRVRRKRMGHINL
                     AAPVVHIWFFKAMPSRLGNLLDMKTSDLEKIVYFQDYVVVDPLDSPLKPKQLLNEEEY
                     RAAREKYGENGFIAQTGAEAIRSLLRSMDLAALSAELLDQIGKTNSKQRLKDLCKRLK
                     IVENLRNSGNEAEWMVMEVIPVIPPDLRPLVLLESGNFATSDLNDLYRRIINRNNRLK
                     KLVDLNAPEVIIRNEKRMLQQAVDALFDNGRCRRPVLGSSNRPLKSLTDMIKGKQGRF
                     RENLLGKRVDYSARSVIVVGPELQLHQCGLPKKIALELYQPFIIRKLRERGLADTIKS
                     AKKMLERRDQEIWDILEEVIVNHPVLLNRAPTLHRMGIQAFEPVLVEGNAIRIHPLVC
                     KGFNADFDGDQMAVHLPLSVEAQTEAHVLMMSTNNIFSPANGSPIMSPSQDIVMGIFY
                     LTMDYSPNLPDPKAKLPCFSSPHEAMMAYEQKKITIHQKIRCRVERAMMVSSQKTDPE
                     PVPANRIVETTVGRLIFNDMLPPEMPLYNYPLSQKGAGRVIADCHHLLGRAATIDLLD
                     RIKATGFKHSTLAGLSFGIHDLRIPAAKWDIISRAQKRVDQIERGYQRGAITPMERHN
                     QLIDVWVHAREQVTKEMMKEFKFDRRDEEGNYVPAGENRGRFYLNPI"
     gene            complement(2270..2425)
                     /locus_tag="P5316_17385"
     CDS             complement(2270..2425)
                     /locus_tag="P5316_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86786.1"
                     /translation="MRGLLAVKVGEPVYNLSFGLCIASSLLPVVLLVWPLRSTGRRNG
                     KKGPDPK"
     gene            complement(2454..6233)
                     /gene="rpoB"
                     /locus_tag="P5316_17390"
     CDS             complement(2454..6233)
                     /gene="rpoB"
                     /locus_tag="P5316_17390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRR86787.1"
                     /translation="MLTPQEVRNFGRLGDTMPVPSLTSIQTESYARFLQQDVPPDQRQ
                     NIGLEALLREVFPIQSYDGSMSLEYVYYDLSKPRYTPDECRQLRLTYGMPFRVHVRLV
                     RKDRDEISEDAIYLGELPVMMGGGEFIINGAERVIVNQLHRSPGVDFIKAQAEGDRAL
                     HGCRIIPERGSWIEINVTKKDVLAVRIDQSAKIAATTFLRAMGPEYASNEAIIKTFYD
                     SKIKNVKAGGLRPDMYAVSDIVDEESGEVLVKGGCQIGDAVQRLQKSGVKSVSVIEQV
                     PDPLILNTMAEDGCHDYESALLKIYAKLRPGNPPQLEKAQKLFHEKFFDENRYRLGKV
                     GRFRLNRKFNQNVPETEMTLRVEDVVNSLRYILLLRSGESAYEVDDIDHLGNRRLRTI
                     DELAAEEMRKGFLKLRRTVQERMSLKDPDNLGRIAELVNSKSISSSIEFFFGRGELSQ
                     VVDQTNPLSQLTHERRLSALGPGGLNRKRAGFEVRDVHISHYGRICPIETPEGTNIGL
                     IASLSIYSTVDDYGFLVTPYRKVEKGKVNGDVAMLRADEEMRAVLAPPESVEPQTGKV
                     VPGPVLARVNGELAMVDSNEIDYIDISPKQVVGVSAALIPFLEHDDANRALMGSNMQR
                     QAVPLLRCEPPIVATGMEKLVPLNSGMVVRASRGGTVTAVDATRIEIDGTDEYILRKF
                     QGLNERTCLNQKPIVSLGQKVKAGDIIADGPACKNGELALGRNVLVAFITFDGYNFED
                     AILISERLLKDDVFTSIHIDEYDIEIRETKLGREEFTRDIPNVSEKALANLDENGIVR
                     IGTRVGPGDILVGKVSPKSKSELSPEEKLLYAIFGRAGEDVKNDSLELPAGEEGVVID
                     TKWFSRRMHLTDEQKKNLGIQMKDYKRQQDTKIVSVFRKMCETIEEVTGQVMVDPSTK
                     QKVGKSESMEVILEQVESFKAQIESGTIKWIKSAPARDAAMKVIDRYWPRIRDIIDER
                     ERKLSQMKRGDELPSGVLEMVKVYVATKRQLAIGDKMAGRHGNKGVIARILPEEDMPF
                     LEDGTPVDILLNPLGVPSRMNVGQILETHLGWAAAILGFQAITPVFDGATEEDINRCV
                     QEANERVRQRREDPQAIQNAGQTREILVEMPKGGKVRLYDGRTGEPFDQPVTVGYLYM
                     DKLHHLVDDKIHARATGPYSLITQQPLGGKARTGGQRFGEMEVWGLEAYGAAYVLQEL
                     LTVKSDDVEGRTKIYESMVKGTNTLEAGTPVSFDVLCNEIKGLGMNIQLEKVRVS"
     gene            complement(6848..7264)
                     /gene="rplL"
                     /locus_tag="P5316_17395"
     CDS             complement(6848..7264)
                     /gene="rplL"
                     /locus_tag="P5316_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRR86788.1"
                     /translation="MADAPAKGFSAETVALAEKIAGLTLKQAQELVDCLKEKYGIEPA
                     GGGAMIVAGAAGAGGEGAAAPAEEKTAFDVILKSGGDKKINVIKVVRAATNLGLKEAK
                     DLVEGAPKPVKQGVSKEEAEKLKKELEEAGAVVEVK"
     gene            complement(7450..7971)
                     /gene="rplJ"
                     /locus_tag="P5316_17400"
     CDS             complement(7450..7971)
                     /gene="rplJ"
                     /locus_tag="P5316_17400"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRR86789.1"
                     /translation="MSKATKDLITCEYAGRYANLANACVVSVIGLDAISTNKLRGELR
                     SMKISLHVVKNSLARRALADGPLAPLASALEGPCALVVGGESIIDVAKALVELKKKYP
                     AIELKKGILEGDPDLLDIERLSQMKSRGDLLAEIAMLIASPGRRLAGCIRGPGGRLAG
                     CFKAIADKQEGSQ"
     gene            complement(8048..8773)
                     /gene="rplA"
                     /locus_tag="P5316_17405"
     CDS             complement(8048..8773)
                     /gene="rplA"
                     /locus_tag="P5316_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRR86790.1"
                     /translation="MPRQSARYKLQLETRGNAARLSIPEAVKLVKSMAAARPEKKDYK
                     GRKKPKGFDQTVELVCWLGVDPRQADQALRGAVSLPKGIGKSKRVIAFCPDDMAEKAK
                     AAGAVDAGADELIKKVNDGWTDFDVAVAHPAVMGKVGKLGRVLGPQGKMPSPKSGTVT
                     PDIEQAVREYSAGKVEFRTDAGGNVHAPVGKVSFPDQDLVENIEAFIAHIRRSKPATS
                     KGQYFKRVCLTATMTPSVTLDVG"
     gene            complement(8905..>9273)
                     /gene="rplK"
                     /locus_tag="P5316_17410"
     CDS             complement(8905..>9273)
                     /gene="rplK"
                     /locus_tag="P5316_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRR86791.1"
                     /translation="APPIGPALGQHGVNIGQFVQQFNAQTGALNGMIVPVEITVYKDK
                     TFTFVVKSPPAAVLLKEAAGVAQGSGTPNKEKVGKVTWSQIRKISERKAKDLNAFDLE
                     HADRIIAGTARSMGIEIVEG"
BASE COUNT         1647 a         2980 c         2653 g         1993 t
ORIGIN      
        1 aaatggggtt gagatagaag cgaccccgat tctcgccggc aggaacataa ttaccctcct
       61 cgtcgcgacg gtcaaacttg aattccttca tcatctcctt ggtgacctgc tcgcgggcat
      121 gaacccatac gtcgatgagc tggttgtgcc gctccatcgg ggtgatggcc ccccgctggt
      181 aaccgcgctc gatctggtcg acgcgctttt gagcccggct gatgatatcc cacttggccg
      241 cgggaatacg caggtcgtga atgccgaatg acaaaccggc cagagtgctg tgcttgaagc
      301 cggtggcctt gattcggtcc agcaggtcga tggtcgccgc ccggccgagc agatggtgac
      361 agtccgcgat gacgcggccc gcccccttct ggctgagcgg atagttgtat aaaggcattt
      421 ccggcgggag catgtcgttg aagatcaatc gcccgaccgt ggtctcgaca atgcgattgg
      481 cgggtacggg ttccggatcg gttttctgcg aggataccat catggcccgc tccacccgac
      541 atcggatctt ctggtggatg gtgatcttct tctgctcata ggccatcatc gcctcatggg
      601 ggctgctgaa gcacggaagc ttggccttcg gatccggcag gttcggcgaa taatccatgg
      661 tgagatagaa aatgcccatg acgatgtcct gggagggaga catgatcggg ctaccgttgg
      721 ccgggctgaa gatgttgttg gtcgacatca tcaggacgtg ggcctccgtc tgggcctcga
      781 cggacaacgg caggtgcacg gccatctggt cgccgtcaaa gtcggcgttg aaacccttgc
      841 agaccagcgg gtgaatgcga atcgcgttgc cctcgacgag caccggctcg aacgcctgaa
      901 tgcccatgcg gtgcaacgtg ggcgcgcggt tgaggaggac cgggtgattc acgatcacct
      961 cttccaggat gtcccagatc tcctggtcac gcctctcgag catcttcttg gcgctcttga
     1021 tggtgtcggc caatccccgc tcgcgaagct tgcggatgat gaacggctgg tagagctcca
     1081 gggcaatctt cttgggcagg ccgcactgat ggagctggag ctcaggtccc acgacgatga
     1141 ccgaccgggc cgagtagtcg acacgcttgc ccagcagatt ctcgcggaag cgcccctgct
     1201 tgcccttgat catgtcggtc agcgacttga gcgggcggtt gctgctgccc agaaccggcc
     1261 ggcggcaccg tccgttgtcg aagagagcgt cgaccgcctg ctgaagcatg cgcttttcgt
     1321 tgcgaatgat gacctcgggc gcgttaagat caacgagctt cttgagccgg ttgttgcggt
     1381 tgatgatgcg acgataaagg tcgttgagat cgctggtggc gaagttgccg ctctccagca
     1441 gaaccagcgg ccgcaggtcc ggcggaatga ccgggatgac ttccatgacc atccattcgg
     1501 cctcattgcc gctgttgcgc aggttctcga cgatcttcag ccgcttgcac aaatccttga
     1561 gccgctgctt gctgttggtc ttgccgatct ggtccagaag ctcggcgctc agcgccgcca
     1621 agtccatgga acgtagaagg ctgcggatcg cctcggcgcc cgtctgagca atgaaaccgt
     1681 tctcgccgta cttctcgcgg gccgcccgat attcctcctc gttcagaagc tgcttgggct
     1741 tgagcggaga gtccagggga tcgaccacga cgtagtcctg gaaataaacg atcttctcga
     1801 ggtcgctggt cttcatgtcc agaagattgc cgagccggct gggcatggcc ttgaagaacc
     1861 agatgtgcac gacgggagcg gccaggttga tgtgacccat ccgcttgcgc cggacgcgcg
     1921 aatgcgtcac cttcacgccg caacgatcgc agatgatccc cttgtgcttc gtgcctttgt
     1981 acttgccgca ggcacactcc cagtcgcgct ccggcccgaa gatgcgttcg cagaacagac
     2041 cgtccttttc cgggcgatag gtacggtagt tgatcgtctc cggcttcttc acctcgccga
     2101 acgaccagct tcgaatgtcg ttgggcgatg ccagcgagat cttcacgctg ccgtagtcat
     2161 tgatccgatc gtaaatgttc tcggccattg ttcagggtcc tttcgaaagg aaccgtttca
     2221 ttcggttgcc aggcttccgt cgtcggctcg cagcggactg cggccgctat cacttcggat
     2281 cggggccctt cttcccgttt cgtctgcccg tgcttcttag cggccagacc agcaggacga
     2341 ccgggagtaa cgatgacgcg atacagaggc cgaaggacag gttgtaaacc ggttcgccga
     2401 ccttgaccgc cagcagccct ctcatatccg atcctcattc ccgtcatccc gcctcagctc
     2461 acacggacct tctcgagctg gatgttcata cccagcccct tgatttcatt gcacagcacg
     2521 tcgaacgaca ccggcgtgcc ggcctcgagc gtgttcgtgc ccttgaccat cgattcgtag
     2581 atcttggttc gaccttcgac atcatcactc ttgaccgtca gcagttcctg caagacgtag
     2641 gccgccccgt aagcctccag gccccagact tccatctcgc cgaatcgctg accgccggtg
     2701 cgggctttgc cgccgagcgg ttgctgggtg atcaacgagt aagggccggt cgcccgggcg
     2761 tgaatcttgt catccaccag atgatggagc ttatccatgt agagatagcc gacggtcacc
     2821 ggttggtcga acggttctcc cgtccggccg tcgtacaacc tgaccttccc gcccttcggc
     2881 atttcgacca gaatctcgcg cgtctggccg gcattctgaa tggcttgggg atcctcccgg
     2941 cgctgccgga cccgctcgtt ggcctcctgc acgcaacgat tgatatcctc ctcggtggct
     3001 ccatcgaaca ccggagtaat cgcctggaag ccaaggatgg ccgccgccca gcccaggtgc
     3061 gtctcgagga tctgacccac gttcatgcgc gacggcacac ccagggggtt gagcaggatg
     3121 tcgaccggcg tgccgtcttc caggaagggc atgtcctcct cgggtaggat ccgggcaatg
     3181 acccctttgt ttccgtgccg acccgccatc ttgtcgccga tggccagttg tcgcttggtg
     3241 gccacgtaga ccttgaccat ctcgaggacg ccggaaggca attcgtcgcc ccgcttcatc
     3301 tgagacagct tgcgttcccg ctcgtcaatg atgtcgcgga tgcgcggcca gtaccgatcg
     3361 atcaccttca tcgcagcgtc gcgagccggg gccgacttaa tccacttgat ggtgcccgac
     3421 tcgatctggg ccttgaagga ctcgacctgc tcgagaataa cctccatcga ttcggatttg
     3481 ccgaccttct gtttggtgga ggggtcgacc atcacttgcc cggtgacttc ctcgatggtc
     3541 tcgcacatct tgcggaacac cgagacgatc ttggtatcct gctgcctctt gtagtccttc
     3601 atctggatgc cgaggttctt cttctgctcg tcggtgagat gcatccggcg gctgaaccac
     3661 ttggtatcga tgaccacgcc ttcctcgccg gcgggcaatt ccagcgaatc gttcttcacg
     3721 tcctcgccgg cccggccgaa gatggcgtac agcagttttt cctcggggga cagctcgctc
     3781 ttgctcttcg ggctcacttt gcccaccagg atgtcaccgg gcccgacgcg tgtgccgatc
     3841 cggacgatcc cgttctcgtc aaggttggcc agggccttct ccgaaacgtt cgggatatcc
     3901 cgggtaaact cctcgcggcc cagcttggtc tcgcgaatct cgatgtcgta ctcgtcgata
     3961 tggatgctgg tgaaaacgtc gtccttgaga agacgctcgc tgatcaggat ggcgtcctcg
     4021 aagttgtacc cgtcgaaagt gatgaacgcg accagcacgt tacgaccgag cgccagctca
     4081 ccgttcttgc aggcggggcc gtcggcgatg atgtcgccgg ccttgacctt ttgtcccaaa
     4141 gatacgatcg gtttctggtt gaggcaggtt cgctcgttca ggccctggaa cttccggaga
     4201 atgtactcgt cggtcccgtc aatctcgatg cgcgtggcgt cgacggcggt caccgtgccg
     4261 ccgcgcgatg cccgcacgac catgccgctg ttcagcggca cgagcttctc catgcccgtg
     4321 gccacgatcg gcggctcgca ccgcagcaga ggcaccgcct gacgctgcat gttggagccc
     4381 atgagggcgc ggttggcgtc gtcgtgctcc aggaacggga tgagtgccgc agacacgccg
     4441 acgacctgtt tcggtgaaat gtcaatgtag tcgatctcgt tcgaatcgac catcgccagt
     4501 tcgccgttga cccgggcgag aaccgggccc ggaacgactt tgccggtctg aggttccacc
     4561 gactcgggcg gggcgaggac cgcacgcatc tcctcgtcgg ctcgcaacat cgcaacatcg
     4621 ccgttgacct tgcctttttc aaccttccgg tagggagtga ccaggaaacc gtaatcgtca
     4681 accgtgctgt agatgctcag cgaggcaatc aggccgatgt tcgtcccttc cggggtctcg
     4741 atcgggcaga tacggccgta atggctgatg tgcacgtcgc ggacctcaaa accggcccgc
     4801 ttgcgattca gcccccccgg ccccagggcc gagagccgtc gctcgtgcgt gagctgactg
     4861 agcgggttgg tctggtcaac cacctgcgac agctcgcccc ggccgaaaaa gaactcgatg
     4921 ctggaggaga tgctcttgga gttgaccagt tccgcgatgc ggccgaggtt gtcgggatcc
     4981 ttgaggctca tgcgctcctg aacggtgcga cggagcttga gaaagccctt gcgcatctcc
     5041 tccgcggcca actcgtcaat ggtgcggaga cggcgattcc caagatgatc gatatcgtca
     5101 acctcataag cgctctcacc gcttcgcagc agcaggatgt aacggagcga gttgacgacg
     5161 tcttcgaccc gcaaggtcat ctccgtctca ggcacgttct gattgaactt gcggttcaag
     5221 cggaagcgcc cgaccttgcc cagacgataa cggttctcgt cgaagaactt ctcgtggaag
     5281 agcttctggg ctttctccaa ctgcgggggg ttgccgggcc tcaacttcgc atagatcttc
     5341 agcagggcgc tctcatagtc gtggcacccg tcctccgcca tggtgttgag aatgagcgga
     5401 tccggaacct gctcgatgac cgagaccgat ttcacgccgg atttctggag acgctgaacg
     5461 gcgtccccga tctggcagcc gcctttgacc agcacttcgc cgctttcctc gtccacgatg
     5521 tcgctgaccg cgtacatgtc gggcctcagg ccgcccgcct tcacgttctt aatcttactg
     5581 tcatagaacg tcttgatgat cgcctcgttg ctggcgtact ccggacccat tgccctgagg
     5641 aatgtggtcg ccgcgatctt ggccgactgg tcgatgcgca ccgcgaggac gtctttcttg
     5701 gtgacgttga tttcgatcca gcttccgcgc tccgggatga tgcggcatcc gtggagggca
     5761 cggtcgccct ccgcctgggc tttgataaaa tcaacgcccg gactacggtg gagctgattg
     5821 acgataacac gctccgcacc attgatgatg aactcgccgc cccccatcat gacgggcagc
     5881 tcacccagat aaatggcgtc ctcgctgatc tcgtcccggt ccttgcgaac cagccgaacg
     5941 tgaacacgga acggcatccc gtaggtcagc cggagctgac gacattcatc cggcgtgtag
     6001 cgcggcttgc tcaagtcata gtagacgtac tccagggaca tgctcccgtc gtagctctgg
     6061 atgggaaaca cctccctgag aagggcttcc aggccgatgt tttgacgctg gtccggcgga
     6121 acatcctgtt gcaggaatct cgcataggac tcggtctgaa tgctggtgag cgaaggtacc
     6181 ggcatcgtat cgccgagccg accgaaattc cgcacttcct ggggagtaag cataccggtt
     6241 cgtcctttcc tgcggccaaa ggtaaagccg cacgcctggc acccgttgcg ggacgccagc
     6301 cgtcatcatc cgacctacgc cgattcgtct tgcgagaagg cgacctcgcc cttcgcgcct
     6361 aacgcatcat gcctcacaca gtcttcagga cggagaaccc aacgcgaggc catctgttcg
     6421 actgacggtc ctgcccccac cgggtcccgt ttgcccggcg cgtcagccga tctcgcctca
     6481 cggaggaggg attatgatga aaattcaggt ccaacccgat agacctcatg gcaccgcgca
     6541 tccgtgggaa agcccagagt cacggttcac gccacttccc ccgcgcccga tacgcgcgca
     6601 ccttcacctt cgtagctcaa aggcattcta tcgctgggca tgctgtctgc caagcccatt
     6661 tcccgataag ccctaccgtg gctaggcccc ccaccgcaac cgggagaccc ttgaccacga
     6721 aacctccgag atcttggtgc ggccacacca accccagctc accgccgggc ggtgcgacaa
     6781 acctaccggc ttctcggctc aagaccctta acaatcataa tactgaggat ttcgtaaggt
     6841 cttgttatca cttgacttcg acaacagcac cggcttcttc aagctccttc ttgagcttct
     6901 cggcctcttc cttcgatacg ccttgcttga ccggttttgg agcgccctcg accaagtcct
     6961 tagcctcctt gagacccaga ttggtagccg cccggacgac cttgatcaca ttgattttct
     7021 tgtcgccgcc gctcttgaga atgacgtcga aggcggtctt ttcctctgcc ggagccgccg
     7081 caccctcgcc gccggcgccg gcggcaccgg cgacgatcat tgccccgccg ccggccggct
     7141 cgatcccata cttctccttc aagcagtcca ccagttcctg ggcctgcttg agggtcaatc
     7201 ccgcgatttt ctccgccagc gcaaccgttt cagcgctgaa tccctttgct ggtgcatccg
     7261 ccatggtatc cagaactccc gtcatcacag tgcagtccgc cgatcaacct gataggtgac
     7321 atcccgttga tgtcatttaa cccgccgagc gggccaatcc gcagcaaccg gctgctgctg
     7381 acaatctctt tgctcgtgct ctctcccagg ttccacaaag ccgttgtcgt cctgcttgcc
     7441 tctcaagcct tactgactac cctcttgttt gtccgcaatc gccttgaagc aaccggccag
     7501 acgcccaccc ggtccgcgga tacagcctgc aagccgccgg ccgggactgg cgatcagcat
     7561 cgcgatttcc gccagcaagt ctccgcggct cttcatctgc gacagccgct cgatatcgag
     7621 cagatccggg tcgccttcca gaatgccttt cttcagctcg atcgccgggt atttcttctt
     7681 caattcgacc aacgccttgg ccacgtcgat gatcgactcg ccccccacca ccagggcaca
     7741 cggcccctcc agcgcgctgg ccagcggggc caggggcccg tcagcgagcg cgcgccgggc
     7801 caggctgttc ttgaccacgt ggagcgaaat cttcatcgaa cgaagctcgc cgcgaagctt
     7861 gttcgtcgaa atggcatcga ggccgatgac gctgaccacg cacgcgttgg ccagattggc
     7921 gtaacggccc gcatactcac aggtgatgag atccttagtc gccttgctca tgttggttgc
     7981 ccttgtatct gtccgccggt cctaaggccg gcacatagtc gtccatacga gtccgtgacc
     8041 ccgcgtatca accgacgtcc agggttaccg agggcgtcat ggtcgccgtc aggcagacac
     8101 gtttgaaata ctggcccttc gatgtcgccg gtttgcttcg gcgtatgtgg gcgatgaatg
     8161 cctcgatgtt ctccacgagg tcttgatcgg gaaagctcac cttgccgacc ggagcgtgga
     8221 cattgccgcc cgcatcggtg cgaaactcga ccttgccggc gctgtactct cgaacggctt
     8281 gctcgatatc cggcgtcacg gtgcccgact tgggcgaggg catcttgcct tgtgggccca
     8341 gaacccggcc caacttgccg accttgccca tgaccgccgg atgagcaaca gctacgtcga
     8401 agtcagtcca accgtcgttg accttcttga tcagttcgtc ggctccggcg tcgaccgctc
     8461 ctgcggcctt ggccttctca gccatgtcgt ccggacagaa cgcaatgact cgcttggatt
     8521 tgccgatgcc cttgggcaat gagacggctc cccgtagagc ctggtcggcc tggcgagggt
     8581 cgacgcccaa ccagcagacc agttcgacgg tctgatcgaa tcctttgggt ttcttccggc
     8641 ccttgtagtc cttcttctcg ggtctggccg cggccatcga cttgaccagc ttcaccgcct
     8701 ccggaatgga gagacgggcc gcgttgcccc gcgtctccaa ctgaagctta tatctggcac
     8761 tttgcctcgg catatgactg cctcccactg aaaaaccgac tcgggcccgt ggtcaggccc
     8821 tgtatcaaca acgcgggggc gtatccgctg ccccgccacg atgccgccgt ccctcgaacc
     8881 gcgagcggac ggccgctttg cccgtcaacc ctctacgatc tcgatgccca tcgaacgagc
     8941 agtaccggca atgatccgat cggcatgctc aagatcgaac gcgttgagat ccttggcctt
     9001 gcgctcgctg atcttgcgga tctgagacca tgtgaccttg ccgaccttct ctttgttggg
     9061 cgtgccgctg ccttgggcga cgcccgcggc ttccttgagc agaacggcag ccggcgggct
     9121 tttcaccaca aaagtgaaag tcttatcctt gtaaacggta atctcgacgg gcacgatcat
     9181 gccgttcaac gcgcccgtct gggcgttgaa ctgctgaacg aactgcccga tgttcacgcc
     9241 gtgctggccc agagccggac cgatcggcgg cgc
//
LOCUS       DAOTPW010000230         9174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6904_length_9174_cov_5.207479, whole genome shotgun sequence.
ACCESSION   DAOTPW010000230 DAOTPW010000000
VERSION     DAOTPW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6904_length_9174_cov_5.207479"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="P5316_17415"
     CDS             <1..574
                     /locus_tag="P5316_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HRR86792.1"
                     /translation="ALPVLPVQILWINMTTAVLLGLMLAFEPKEPGIMARPPRNPDQP
                     IITFDVALRMFLIAGVMLLGGFGIFKWELYRGLGEAAARTAAVNVLVLVELFYLFNCR
                     SLTRSMLEIGLFSNRWIPVGVVSMLLLQLAFNYLPAMNRLFHSAPVDAITWFEAAAVA
                     LAGSVLVAMLKRFSRRKRGANAALASGSAS"
     gene            647..1441
                     /locus_tag="P5316_17420"
     CDS             647..1441
                     /locus_tag="P5316_17420"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRR86793.1"
                     /translation="MTSKKQHSIAEAVGNHEPPTVATVRRHPPAGLRQRMLAGLLFVL
                     PFVVTAWIVYWLYSFLENLVIGPTARLVVQLIERRVDVALPSWFVNYVAPLIGVVAVL
                     VLLYFLGFFARVRADRILDAVLLRVPIITSIHKAVSQAFATFRGSNELSRFRRAVLVA
                     FPHPGMRVPGFVTASCRDRLTQKTILCVYVPTTPIPTSGYVLLVPEEEVTELNWDLEQ
                     TVQAVVSFGITAPAEVSYHPSAALDEGPRSLPTEQGPAAPQRSDAS"
     gene            complement(1600..2229)
                     /gene="rdgB"
                     /locus_tag="P5316_17425"
     CDS             complement(1600..2229)
                     /gene="rdgB"
                     /locus_tag="P5316_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRR86794.1"
                     /translation="MREILLATGNAGKAREMKEILSGVGDEICRRVRWRHLQEFTGWP
                     EAIEDGRTFLENAQSKALYYARLSGLWTIADDSGLEVDALGGEPGVRSARYAGEPKSD
                     QANNALLVERLAGVPDDQRTARFRCAVALADGRQVLASAEGTVEGRIIDHPRGANGFG
                     YDPHFLVVSAGMTAAEMPPEQKHAVSHRGQALRKLAVQLAALLSERAVY"
     gene            complement(2268..3122)
                     /locus_tag="P5316_17430"
     CDS             complement(2268..3122)
                     /locus_tag="P5316_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86795.1"
                     /translation="MQQQMSILGRLALRLGPMVLAALLLGSGCDSAGSSGGVWTAQPG
                     AGAEKWTINCFRSAAPNHVELCNGLADLLRKTNGIDPKAVRVQSDALGSTIYYGEYTR
                     VPGAGGQLVFPPKMQEDIKRIRLLTYNQTLPFGLAAPESLGGAAPAGGAGQYNLTTAR
                     GKYSLLIAVFFNTPTFNQRREVAEQYVQQLRSEGVEAYVFHEQFKSYVFVGSFGEDAL
                     TFLPNGTWQVSKEVQDFAAARAGEEFLYLMENGYRQKRVGPDGESILVPSQLMPIPRP
                     GATSLHDR"
     gene            complement(3128..3865)
                     /gene="rph"
                     /locus_tag="P5316_17435"
     CDS             complement(3128..3865)
                     /gene="rph"
                     /locus_tag="P5316_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRR86796.1"
                     /translation="MPALRSDGRRVDELRPVRIIRGYTRCSPGSVLIRTGNTIVLCTA
                     SVADEVPEWLAGKGRGWVTAEYDMLPGSTGRRRDRSRKRLDGRTTEIQRMIGRALRAV
                     VDMEMLGERCIWLDCDVIQADGGTRTAAITGTFVALCDAVGWMRKQGMIGRSPIIEPV
                     AAVSAGKVDGRILIDLDYSEDSRAEVDLNVAMTASGSLVEVQGTGEGGAFTRKELDRM
                     LDRAAVGVRRLIEAQRRALRRRPGGGR"
     gene            complement(3903..4862)
                     /gene="argF"
                     /locus_tag="P5316_17440"
     CDS             complement(3903..4862)
                     /gene="argF"
                     /locus_tag="P5316_17440"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRR86797.1"
                     /translation="MSTEIPDCLQSLKHFLAIQDLSAEALQALIDFSIRRKGQFKRGE
                     LKPVLERKVLAMLFQKSSLRTRLGFETAMVQLGGYAVCLEDHQIGVAKREEPKDIARV
                     MSSMCDGIMARVKAHQILIELAARSTVPVINGLSDWSHPCQALADVMTLQEHFGSVAG
                     RKLAFIGDGNNVARSLLSASAKLGMSFALAAPKKYQFDDGVIQSAHDKAGKNGAAIVQ
                     TEQPEEAAADADVLYTDVWTSMGQESERAAREADFAGYQINAALVKKAKPKAVVMHCL
                     PAYRGLEITDEVIDGSQSLVFQQAENRLHSQRALLEVLLGRRK"
     gene            complement(4859..6058)
                     /locus_tag="P5316_17445"
     CDS             complement(4859..6058)
                     /locus_tag="P5316_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRR86798.1"
                     /translation="MTSQEVFDLYSRCVIGNYGRLPVVIVKAQGSQMWDIDGKRYLDL
                     FPGWGCSLLGHCHPRVVEAIREQVGRLIHVDNTFYTVEQGRLAEMLSRHSFGGQCFFS
                     NSGAEANEGAIKLARKHAGQGRYRIITMEKSFHGRTFGAMSATAQSKTHAGFEPLLPG
                     FVYVPFDDVDAVARAITDETAAVMLEPVQGEGGINIPSDDYLPKIRELCDEHNLLLIL
                     DEVQTGVGRTGKWFGYQHSNIEPDIVTMAKALGNGAPIGAIIAQPDVAAAFTPGSHAS
                     TYGANCLVVAAAIATLETIESEGLLERVDRISAYIVDRARAMQQRFGFIEEVRARGLM
                     IAIQLSLPGAAIVSSALEKGLRINCTQGNVIRLLPAMTITTQEVDEAFEIMTAAFAES
                     ESKLVTT"
     gene            complement(6501..7079)
                     /locus_tag="P5316_17450"
     CDS             complement(6501..7079)
                     /locus_tag="P5316_17450"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRR86799.1"
                     /translation="MSFLTRILFEDLAVLLIIEFLVLMSVLAVHRQRMTPRTRRAVWI
                     TLGCCAAFITLNHLVTTDSERIVQTVSAIAKAVDEGDVPAIANHLDDEFHYRNWDKDG
                     FVAELNGKLQQWRIDEAKVGRFEIRIEGDAATASFRASCDWRGASDAQAGVASTWTLE
                     LIRRPGGWKLRRVVSAKVGPGYKLDLNEVWHY"
     gene            complement(7184..7528)
                     /locus_tag="P5316_17455"
                     /pseudo
     CDS             complement(7184..7528)
                     /locus_tag="P5316_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(7563..8615)
                     /locus_tag="P5316_17460"
     CDS             complement(7563..8615)
                     /locus_tag="P5316_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRR86800.1"
                     /translation="MLRIGSLTLEFPVIQAALSGYSDLPMRRVARMLGASYMLSEVVL
                     DRLVIQKGRKRRTITTVHPDDHPVGGQLMGAEPNAFAQAADALVQAGYDVVDINFACP
                     VRKVLGRCRGGYLLSDPETALAIVRAVVEAVDHRRPVTLKMRRGMDDTPESERKFFTI
                     LDGAFAAGIDAVTVHPRTVRQRYVGPSQWPFLKRVKQHVGDRVVIGSGDLFCAEDCIR
                     MMAETGVDGVAVARGCIGNPWIFRECRELLAGRPIPPAPSVPEQGEVIARHYAWTAEV
                     YGDKIAGKMMRKFGIKYSELHPMDREVRDAFIAARTRVDFQAVLTQWYDPARDWPPGV
                     RRTGPGHLVAAGARMG"
     gene            complement(8728..9087)
                     /locus_tag="P5316_17465"
     CDS             complement(8728..9087)
                     /locus_tag="P5316_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRR86801.1"
                     /translation="MRDHTKLKVFQAADRLAILVYKMTRTFPREEQSGLTSQMRRAAV
                     SVPSNIVEGCARPTEANYIRFLGTSFASLRELQYQGSLVHRLGYCKDDARQAFDALCT
                     EVSKMLGAMIRSLRSEQ"
BASE COUNT         1688 a         2890 c         2792 g         1804 t
ORIGIN      
        1 ggccctgccc gtgctccccg tgcagatcct ctggatcaac atgaccacgg cggtactcct
       61 gggcctgatg ctggccttcg agcccaagga gccgggtatc atggcgcgcc cgccccgtaa
      121 cccggaccag ccgatcatca ccttcgatgt ggcccttcgg atgtttctga tcgccggggt
      181 tatgctgctg ggcggcttcg gcatcttcaa atgggagcta tatcgtggtc tcggcgaggc
      241 ggcagcccgg acggctgcgg tgaacgtact cgtcctcgtt gagttattct acctgttcaa
      301 ctgccggtct ctgacacgtt cgatgctgga gatcggtctg ttttccaatc gctggatccc
      361 ggtgggggtt gtgagcatgt tgctgctgca actggcgttc aactatctgc cggcaatgaa
      421 caggctgttt cacagcgcgc ccgtggatgc aatcacctgg ttcgaggccg ctgcggttgc
      481 ccttgccggt tccgttcttg tggccatgct gaagcgattc tctcgccgca agcgaggcgc
      541 aaacgccgcg ttggcgtccg gttccgcgtc gtaaatccgg cgcatgccgc ctcgggccca
      601 cgcgggcggc gcggagtgcg gcgccgacat atgaaggacg cgagccatga cttccaagaa
      661 acaacattcg atcgcggaag cggtcggcaa ccatgagccg ccgaccgttg caaccgttcg
      721 gcgccacccg ccggccggcc ttcggcagcg catgcttgcc ggcctgttat tcgtcctgcc
      781 gtttgtggtc acggcatgga tcgtgtactg gctgtactcg ttcctcgaaa accttgtcat
      841 aggtcccacg gcgcggctcg tggttcaact gatcgagagg cgcgtggacg ttgcccttcc
      901 ttcctggttt gtgaactatg ttgcccccct gatcggcgtg gtggcggtcc tggtattgct
      961 ctactttctc ggcttctttg cacgggtccg ggcggatcgc attcttgatg cggttttgtt
     1021 gcgggtgccc atcatcacat ccattcacaa ggcggtcagc caggctttcg cgacgtttcg
     1081 agggtcaaac gagctgagtc gctttcgacg tgccgtgctt gtggcgtttc cccatcctgg
     1141 gatgagggtt ccggggttcg ttacggcctc ctgccgcgac agactcacgc agaagacgat
     1201 tctgtgcgtc tatgtgccga ccacacccat cccgacctcc gggtacgtgc tgctcgtccc
     1261 tgaggaggag gtgaccgagc tgaattggga cctcgaacag accgtgcagg ccgtcgtttc
     1321 tttcgggatc acggcgccgg cggaagtcag ctaccatccg tcggccgcgc tcgatgaggg
     1381 cccgcgatcg ctgcccaccg agcagggtcc ggccgctccg cagagatcgg acgcatcgtg
     1441 aggattcgtt tgaccggttg ccgaccgcgc ggatgaacaa cttgcccgtt tccagacctc
     1501 ataccgcaag gtatcgcctg gtcccgatag ggcgcagaca agagacggat gaatgctttc
     1561 gccatccgcg gaagacttga ccccggtgca gggcgcgtct caatacacag cccgttcgga
     1621 caggagtgcg gcaagctgga cagccagctt ccgcagagcc tggccacggt gactgacggc
     1681 gtgtttctgc tcggggggca tctcggctgc agtcatgccg gcagaaacga ccaggaaatg
     1741 gggatcatag ccgaagccgt tggcaccccg cggatggtcg ataatccgcc cttccaccgt
     1801 cccctcggca ctggccagca cttggcggcc gtcggcaagc gcaaccgcgc agcggaacct
     1861 tgccgtgcgt tggtcatcgg ggacgccggc cagccgctca accaggaggg cattgttggc
     1921 ctgatcgctc ttgggctcgc cggcataccg ggcggatcgc acgccgggct cgccacccag
     1981 ggcatcaacc tccaggccgg aatcgtcggc gatcgtccag aggccgctca gtcgggcgta
     2041 gtacagcgcc ttgctctggg cattctccag gaacgtccgg ccgtcctcga ttgcctccgg
     2101 ccaccctgtg aactcctgaa ggtgccgcca acgaaccctg cggcagattt cgtcgcccac
     2161 cccgctcagt atttctttca tttccctggc cttaccggcg ttgccggtcg ccagcaggat
     2221 ctcgcgcatg gcatgctccg cggaatatca tctcagatga ggttttccta ccggtcgtgc
     2281 agactggtcg ctccgggacg tgggatcggc atcaactggg acgggacaag aatagactcg
     2341 ccgtcggggc cgacccgctt ctgacgataa ccgttctcca tgaggtacag gaattcctcc
     2401 ccggcccgcg cggctgcaaa atcctggacc tccttgctga cttgccaggt cccgtttggc
     2461 aggaaagtca gggcatcttc tccaaaggac cccacaaaga cgtaactctt gaactgctca
     2521 tgaaaaacat aggcttcaac gccctcagat cgaagctgct gcacgtactg ttcggcgact
     2581 tcgcggcgct ggttgaaggt gggggtgttg aagaaaaccg cgatcaacag ggagtacttg
     2641 cctcgcgcgg ttgtcaggtt gtactggccc gcgccgccgg caggagccgc acccccgagc
     2701 gattccggtg cggccaatcc gaacggcaag gtttgattgt acgtcagcag gcgaattcgc
     2761 ttgatatcct cctgcatctt aggcggaaac accaattggc ccccggcacc gggcacacgg
     2821 gtgtattcgc cgtagtagat ggtgctgccc agcgcgtcgg actgtacgcg gaccgccttg
     2881 ggatcgattc cgttggtctt tcggagcaag tccgccaggc cgttgcacaa ctcgacatga
     2941 ttgggagcgg cggagcgaaa gcagttgata gtccattttt cagcgccggc tccgggctgc
     3001 gcggtccaga ctccgccgct gctgccggcg ctgtcgcagc ctgagcccag caggagcgcc
     3061 gccagcacca tggggccgag ccgaagagcc agccttccga gaatcgacat ctgctgttgc
     3121 atgtttgtca ccgccctccg cccggccggc ggcgcagcgc acgccgctga gcctcgatca
     3181 gccgacgaac accgacggcc gcccggtcca gcatccggtc cagttccttg cgggtgaacg
     3241 ccccaccttc gccggtgccc tgcacttcca cgaggctgcc ggaggccgtc atggcaacat
     3301 tgagatcgac ctctgcgcgg gagtcctcac tgtagtcgag atcgatcagt atcctgccat
     3361 cgacttttcc cgcgctgacg gccgccacgg gttcgatgat cggggatcga ccgatcatcc
     3421 cttgttttcg catccacccg acggcgtcac ataaggccac aaaggtaccg gtaatcgcgg
     3481 cggtgcgcgt gccgccgtcg gcctggatca catcgcaatc cagccagatg caccgttcgc
     3541 cgagcatctc catgtccacg accgcgcgca gggcccggcc gatcatgcgt tggatttcag
     3601 tggtgcgtcc gtcaagccgc ttgcgactac ggtcgcgccg ccggccggtt gagccgggca
     3661 acatatcata ctcggccgtc acccaccccc gacccttgcc ggccaaccac tccgggacct
     3721 cgtcggccac gctggcagta cataatacga tcgtattgcc cgtgcggatc aagaccgaac
     3781 ccggcgagca ccgggtgtag ccgcgtatga tccgaaccgg gcgcagctca tccacgcgac
     3841 gaccgtctga acgaagagca ggcatgactg actttcccgt aaacaaccag gctgccctgt
     3901 tctcactttc gtctgccaag gagcacttcc agcagggccc gctgagaatg taaccggttc
     3961 tccgcctgtt gaaagaccag cgactgtgag ccgtcgatca cttcatcggt gatttccaga
     4021 ccccggtagg ccggcagaca gtgcatgacc accgccttgg gtttggcttt cttcaccaaa
     4081 gcggcattga tctgatagcc ggcgaaatcg gcctcgcggg cggcccgttc ggactcctgt
     4141 cccatgctgg tccagacatc ggtgtacaac acgtcagcgt cggcggccgc ctcctcgggt
     4201 tgttcggtct gaacgatggc ggccccattc ttgcccgcct tgtcgtgagc cgactgaatg
     4261 acaccatcgt caaactggta tttcttcggg gcggcgagcg cgaaactcat gccaagcttt
     4321 gcgcttgcac tgagcagaga gcgggccaca ttgttgccgt cgccgataaa cgccagtttc
     4381 ctgcctgcga cactgccgaa gtgttcctga agggtcatca catcggcgag cgcctggcaa
     4441 gggtggctcc agtcgctcag gccgttgatg accggcacgg tgctgcgggc ggccagctcg
     4501 atgaggatct ggtgggcctt cacccgggcc atgattccgt cacacatgga ggacatgact
     4561 cgggcgatat ccttgggttc ttcgcgcttg gccacgccga tctggtggtc ttccaggcag
     4621 acggcataac ccccgagttg gaccattgcc gtttcaaagc ccagacgcgt tcggagggag
     4681 gacttctgaa aaagcatcgc gagcacttta cgttccagga ccggtttcag ctcgcctcgc
     4741 ttgaactgcc ccttgcgacg gatggagaag tcgatcaagg cctgcaaggc ctccgcgctc
     4801 agatcctgaa tggccaagaa gtgcttcagt gattgcagac aatcggggat ttcagtgctc
     4861 atgtggtcac cagtttgctc tcggattcgg caaaagccgc ggtcatgatc tcgaaggcct
     4921 cgtcaacctc ctgcgtggtg atcgtcatcg ccggcaggag ccggatgacg ttgccctggg
     4981 tgcaattgat gcgtaagccc ttttccaggg ccgacgaaac gatcgcggct ccgggcagcg
     5041 acagttggat ggcgatcatc aagccgcgag cccggacctc ctcaatgaaa ccgaatcgct
     5101 gttgcatggc gcgggcgcgg tccacgatgt aggccgaaat tcggtccaca cgctccaaaa
     5161 ggccctcgga ctcgatggtc tccagggtgg caatggccgc cgccaccacc agacagtttg
     5221 ctccgtaggt cgaggcgtgc gagccggggg taaaggcggc ggcgacatcg ggttgggcga
     5281 tgatcgcgcc gatgggcgcg ccgttgccca aggctttagc catggtgacg atgtccggct
     5341 cgatgttact gtgctgatag ccgaaccact tgccggtccg accgacgccg gtctgcactt
     5401 cgtccagaat cagaaggagg ttgtgctcgt cgcacagttc gcggatcttc ggaaggtagt
     5461 cgtcggaggg aatgtttatt ccaccttctc cctgaaccgg ctcaagcatg acggcggccg
     5521 tttcgtcggt gatggccctg gcaaccgcgt ccacatcatc gaacgggacg tagacgaagc
     5581 ccggcaacag aggctcgaag ccggcatgcg tctttgattg ggcggtggcc gacatggccc
     5641 cgaaggtccg gccgtggaag ctcttttcca tggtgataat cctgtagcgt ccctggccgg
     5701 catgcttgcg agccagtttg atggccccct cgttggcctc agccccgctg ttggaaaaga
     5761 agcactgccc gccgaagctg tgccggctga gcatctcggc caggcgaccc tgctcgacgg
     5821 tgtaaaaggt gttgtccaca tggatcaggc ggccgacctg ctcgcggatc gcctcgacga
     5881 cgcggggatg acaatggcct agcaggctgc agccccagcc agggaacaga tcgaggtacc
     5941 gcttgccgtc gatgtcccac atctgcgaac cctgggcctt gacaatcacc accggcaggc
     6001 ggccgtagtt gccgatcaca catcggctgt acagatcaaa tacttcctga gaggtcatct
     6061 atagcactcc gtagcactca tctgcgaccg gtccggccgg ccgagaaaga aactcttatt
     6121 gtacccccgg ttccggccat gtggcaacgg ccggaaaatc ggagatcttc gcatgagggc
     6181 gcatccctga aacgctggca cgtgtgccca tgcccgctgc gagtgcggca gtgtcgaggt
     6241 cgcttcagcg accgagacct gccgagaacg gccggaatta tcggcaggtc tcatccgctg
     6301 cgcggatttc gaccatgccc tactttcaca acgccaccga aaacggcatg caccctcgca
     6361 cgacggtgca tccaccagat tgcggtggtt cgcggagcgg gcaagtgccg tcgtgcggca
     6421 ctgccgtgtc gccgcccgcc cgtcagcact gctcgcagag caatggcaca cagccctttc
     6481 caggggtgcc gagtgatcga tcagtagtgc cacacttcgt tcaggtcgag cttgtatccc
     6541 gggccaacct tggcgctgac gacgcgtcgc aacttccatc cgccgggccg gcggatcagc
     6601 tccagcgtcc aggtgctggc tacgccggcc tgcgcgtcgc tggcacccct ccagtcgcag
     6661 gacgcccgga aggaagcggt ggccgcatca ccttcgatcc ggatctcgaa ccgccccacc
     6721 ttggcctcgt cgatccgcca ttgctgaagt ttgccgttca gctcggccac gaagccgtcc
     6781 ttgtcccagt tgcggtagtg aaactcgtcg tccagatgat tcgcaatcgc gggcacgtca
     6841 ccctcgtcga cagccttggc gatggccgag acggtttgga cgatccgctc gctgtcggtg
     6901 gtcaccagat ggttgagggt aatgaaagcc gcgcagcaac ccagtgtgat ccacacggcc
     6961 cgacgggtgc ggggtgtcat gcgctgccga tggacggcca gcacggacat gagcacgaga
     7021 aactcgatga tgagcaggac ggcgaggtct tcgaagagga tgcgggtgag gaaggacacg
     7081 attgggctcc aaaggtcatt ggtgtactgg tctgttggtc tgttagtctg ttggtctgtt
     7141 ggtctgctgg cgcgtttgcg ttcgttgcac atcgccggtt gtgcatggag cggattagtc
     7201 cagccaagag ctttgagacc ttcgtacact gggcatcgag agcacagaaa cgcttttgat
     7261 ccaggtatcc cagaaggcga gacagagacg cttggtagtg caattcccgc aaagacgcga
     7321 acgctgtatc caggaaccgg acgaagtcgg cctgactctg tcgagcgcag tcttcgacta
     7381 tgtttgaagg cacagacaca gcggctcgcc tcatctgggc agtgagccca aactgctcct
     7441 ctcgaggaaa aggcttggtt gccctgtaga tgaggacggc caactcatca gcttcctgga
     7501 atgcctgaag tgtggtgtga tctcgcatat tcgatactca ttacccccca ccaacagacc
     7561 aattacccca tcctcgctcc cgctgccacc agatgtcccg gaccggttcg gcggacaccc
     7621 ggcggccagt cgcgggccgg atcataccat tgtgtcagga cggcctggaa gtcgacacgg
     7681 gtcctggcgg cgataaaggc gtcgcgcacc tcgcggtcca tgggatgaag ctcgctgtac
     7741 ttgatgccga acttgcgcat catcttcccg gcgatcttgt cgccgtagac ttcggccgtc
     7801 caggcatagt ggcgggcgat gacctccccc tgctcgggaa ccgacggagc cggcgggatc
     7861 ggacggccgg ccagcagttc gcgacattcg cggaaaatcc acgggttgcc gatgcagcct
     7921 cgggcaacgg ccactccgtc gacgccggtc tcggccatca tgcggataca atcctcggcg
     7981 caaaacaggt caccgctacc aatgacgacc ctgtcgccaa cgtgctgctt gactcgtttg
     8041 aggaaaggcc actgactcgg gccgacatag cgctggcgaa cggtgcgcgg gtgcaccgtc
     8101 accgcatcga tgccggcggc aaaggccccg tcgagaatgg tgaagaactt gcgttcgctc
     8161 tcgggcgtgt cgtccatgcc gcgccgcatt ttgagcgtca ccggccggcg atgatcgacg
     8221 gcttcaacga ctgcccgcac gatcgccagc gccgtttccg ggtccgagag cagatagccg
     8281 ccgcggcaac ggccgaggac ctttctgacc ggacaagcga agttgatgtc caccacgtcg
     8341 tagccggcct gcaccaaggc gtccgctgcc tgggcaaagg cgttcggctc ggcccccatg
     8401 agctgcccgc cgacaggatg atcgtcagga tgaacggtgg taatcgtccg gcgcttgcga
     8461 cccttttgga tcaccagccg atcgagcacc acctcgctga gcatgtagga cgcgcccagc
     8521 atccgcgcca cccgacgcat gggcagatcg ctgtaaccgc tcagagcggc ctggatgacg
     8581 gggaactcga gtgtgagcga gccaatgcga agcatgtatg gattttatcc ggaaccaaga
     8641 aagactgtta ggctgttgga ctgttgggct gttaggccag tgcaagatcg ttggacctta
     8701 tagtcgctca gacgaacaga agaaccatca ctgctcgctc cgcaaagagc ggatcatcgc
     8761 gccgagcatc ttggatacct cggtacagag tgcgtcaaag gcctgacggg cgtcgtcctt
     8821 gcagtagccc agacgatgga ccagcgaacc ctggtactgc agttcacgaa gggaggcaaa
     8881 tgaggtgccc aaaaagcgaa tgtagttggc ctccgtcggc cgcgcacatc cctccacgat
     8941 gtttgacggg acggagaccg cggctcgtct catctgcgag gtcaggccag actgttcctc
     9001 tcgtgggaag gtccgcgtca tcttgtacac aaggatggct aacctgtccg ccgcctggaa
     9061 taccttgagc ttggtgtgat ctcgcaagga actaacctcc agattgcctg cttcaccgag
     9121 agtctaacag actaaggcgg accgcctttt tgcaggccgc aacccaaccg ctct
//
LOCUS       DAOTPW010000231         9089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6961_length_9089_cov_5.065309, whole genome shotgun sequence.
ACCESSION   DAOTPW010000231 DAOTPW010000000
VERSION     DAOTPW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6961_length_9089_cov_5.065309"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            502..720
                     /locus_tag="P5316_17470"
     CDS             502..720
                     /locus_tag="P5316_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86802.1"
                     /translation="MRGHDSFDTFAGSYNLASFGLAPGEMPLQLRLTNPDDPVFYLDD
                     MMIATRAPEPASLILPALDAAFLPRRLK"
     gene            1205..1288
                     /locus_tag="P5316_17475"
     tRNA            1205..1288
                     /locus_tag="P5316_17475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1239..1241,aa:Leu,seq:caa)
     gene            1555..5205
                     /locus_tag="P5316_17480"
     CDS             1555..5205
                     /locus_tag="P5316_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86803.1"
                     /translation="MATNAQPDQHAPAASSESDAGSQALGQAPPQSKRRRRRWWIRLS
                     LGILLLLIILVLLAPYLVSTAIGSRLILSLVNDQIEGRVDLERLSLSWGGPTKLTGLT
                     VLDPAGREVLTVEQAVWTGGAWTALRSRQQLGEISVDSPQAVLYIDEHNRISLVQAFS
                     LRKPAVSTKPISVPPVTGNVSLNKGSVRLVRVDGRTYDVPQLNAKCHVDAMQDLKGTI
                     EATLADASTVAFDASVRQFVTKGQLDVSQASGQIRVATGGDVDIAGLAQFLLDQASAA
                     GRAHFDGGLTFKPGEALVDLKSHATAIQITRANTPAINPTDLAIESHSRLTAEAMSAD
                     VSLTGQAGNAHVRLAYAPSGQPVRISPQQLLSAALTGNPIAMPDLTADAGANLNLAAL
                     AQAIPALLTIRPGVQITGGQLGIEDLSIRGGAQPAMKGTLRLAELTAVDNGRTTRWEP
                     ITAEFDVSLEAGQGLKIRQTQVESGFARVIAGGSASDLHADLTADLAKMGQQLGQAVD
                     VGAKVPAGFITGTLRVKRAGDDRMDFACDLVASDLRYKTDKGQLDAAKATLKQSGHLQ
                     LAGAEVTRLVTSSFTADVDGQVVATGSGWFDLQRGTYSVDADVENADLAYLGSKATGF
                     GVEGLNRYAGTGVIHVEIDRASPDGPIVSGGQATLRNTTVDGEPLAGQDVVLAWSEAS
                     WSPEPGALSVNTAEFRSDLAHATARQVLLKTREGLTLNGKVEAIADLAQCFATAGRIG
                     QWKTLPRIAGRLALTGTCASASGTIATSGNCRIESLEIGAGKQAIREDQLQLVYDAAV
                     NTRERNITLKQCELASRLLSAKMTGTIDHYATACVLSLSGSYEGSWDSMTAVIHELVP
                     ATRDSLSLSGTTAGAFTAKGAAYQPETRPVYRGVAAGLDVGWTSVKAYGVTLGQAQLS
                     PVLRDGELLIPVTAIPASVGQVRLGGLIDLRTSDPTFRLPGRVLVVEGAQVTPEVGRH
                     VLSRINPIFAFMTRAEGTLSLATEDIVLRLSDQIRQSGSGKGRLDLKDFKVQFGGPMA
                     VLLELGAMDERDMLAVTMDGVDFALKDGRIRYDNLTMVFPENFDLKFSGSVGLDETVD
                     LTVSVPVRAALLERFGVRGPVTEYARLLEGARVAVPMTGHRLLPKVDLSRVDMKPLVE
                     RAMRAAVTREAPGVIDALRKPKQDSTDKTPTKQPPPAQPTTKPIEDKAKRLLDELLKD
                     RKKTDKPAPKKR"
     gene            complement(5295..7073)
                     /locus_tag="P5316_17485"
     CDS             complement(5295..7073)
                     /locus_tag="P5316_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRR86804.1"
                     /translation="MHLLDWIVIALYFVILLGLAWWVILRTKNTADDYFLAGRHLGWF
                     VIGASIFACNIGSEHLVGLAGSGATDGVTLAHYELHAWCVLVLAWVFVPFYARSRVFT
                     TPEFLEKRFSPKNRLLLSLISLFAYVLTKIAVGIFAGGVVYSALLPDASLDIGGIHLD
                     SFWVGSILVVLLTGIYTVLGGMRAVAYTEALQTFILIFGSAAVTFFGLKAIGGWGHLR
                     EVCGSEMFNLWKPLVPPGVEAAWSPVEVKDAAGQVVRQAWYFNTNYPWIGMLFCAPIV
                     GLWYWCTDQYIVQRALGAPNETQARRGAIFAGVLKLLPVFLFIIPGMICFALAKSGMQ
                     ELHDGLYNNGQLDRFKAQAAYPLLVQHILPVGVRGLVVAGLLAALMSSLAGVFNASST
                     LFTIDFYSKFRPRASQAELVWVGRMATVAMVLISLIWIPVVRGQKGLYDYLQTVQAYL
                     GPPIFAVFFLGVFFRRLNAAGCLAALVVGFALGVFRLAVDTPVALKLAGFENGYPSGS
                     LFWIVNKMNFQYYSVIIFVVSALVMTLVSYVTRAPDYERIRGLTYGTITDRDRLETRR
                     SYDWRDIASTALVLCVILAAYLYFNG"
     gene            8168..8240
                     /locus_tag="P5316_17490"
     tRNA            8168..8240
                     /locus_tag="P5316_17490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8201..8203,aa:Gly,seq:tcc)
     gene            8270..8533
                     /locus_tag="P5316_17495"
     CDS             8270..8533
                     /locus_tag="P5316_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86805.1"
                     /translation="MREAAGVRQCGLKRRIAGCHTALCVWEVAYAENVAYRSRKGTSQ
                     ALVTLTDWVTKKRRDYWLGEYATPESGEAHHRIIADWTIGGCR"
     gene            8724..>9089
                     /locus_tag="P5316_17500"
     CDS             8724..>9089
                     /locus_tag="P5316_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86806.1"
                     /translation="MRLLRDETIRGSETADPPRQPWSRKYTNEQVKRLRQLFEWAAAQ
                     QLAPVIVYQSCGGPPFCGPQPERTAIQKHARKPHRSLAVRFCCEPWLAGIRRGLWCFG
                     NPGMFFDKSIREIGLPLAGL"
BASE COUNT         1925 a         2813 c         2766 g         1585 t
ORIGIN      
        1 acttatggag gatgcccctc tcttgtcaat ttccacagat aatgagacta tctcggcctc
       61 gaccagcagc agggaaaccg ggtgttcacc cgggaactgc tgaagtgtgt cgaccatttg
      121 gctttggaac cttacggcca ggcatgcgtg cagcttgcgt gtgtgtttct gaatcgcagt
      181 gcgtttgggc tgcggcccgc aagaaggcgg tccgcctttg gggagacaga tcatgtgctc
      241 actcgaatct tgtcatcgtt gtcgcggttg ccaggtgttt ccgctgtccg gctctggtgt
      301 cggcctgctg tgcctgacag tgcttgtcct cctgggagga cagcgagtct gccaggctga
      361 gggattccac tacgactaca acgcctctcc gatacatctg cagatcacgc cgccggaggg
      421 taatcggtcg gggctgtaca tcgaccgcac ggtgaacctg tgcgacgacc cggtcggtag
      481 ctgggatgat ctgaagcttc gttgcgggga cacgacagct tcgacacctt cgccgggagt
      541 tataacctcg ccagcttcgg gctggcgccc ggcgaaatgc ccctgcaatt gcgcctgacg
      601 aaccctgacg atccggtctt ttacctggac gacatgatga tcgccacgcg agcgccggag
      661 ccggcatccc tgatccttcc ggcgctggac gctgccttcc tgccgcgccg gctgaagtga
      721 ctcacatcga gcaaccggat gcgtggacca caggttgtga attggatgac gtacggctct
      781 ggtacgctca aatcttgagc aactgaatcg cttggggtgc tcttcggcgt acgtgggcag
      841 cggcacaaag agactgggca tgcgcacatc gtcgtggttc aggacaccga cggcgtctgg
      901 cggtcggcct ggagcggggc ggccatgaca ttcggggcga ctctctcaca aactcgatcg
      961 taagcaggac ccatacaaca aagcgacgac gccgcaaggg gatcgttccc tcacgatcgc
     1021 ctcggcgaaa ggggggtcgc agtcctgaag gaggggcatg aagcctcacg aggccaagag
     1081 ggaggaccgg cctgttgcct gcttgcctcg cagccctccg gatgttaacc tgtttttacg
     1141 ttcaattggg cgttcgcggg gaacgaggcc cccgaggcaa gcacccccga aaggacgcct
     1201 cgtcgccgaa gtggcggaat tggcagacgc gctggattca aaatccagtg cccgcaaggg
     1261 catgagggtt cgactccctc cttcggcaat cacacatgac ccatgccaat gacggccttt
     1321 cgcggaatcg agcatgtctg ccagtttcaa tgcccgaaca ccccttccgg ctgccggcgg
     1381 tacgccacga cgaacgaagt agttgagaac atgaggctgc tcttcctgcg atgatctgcg
     1441 aagccccctg cccggccgaa ggcagactcg tcgctgatat cgagccgaag ccggatgtgt
     1501 tatcattacc ccaggacaag tcacaataac cgccgccaag gagaacgtcg actgatggcc
     1561 actaatgccc aacccgacca gcatgcgccc gctgcttcct cagaatccga cgccggctca
     1621 caagcccttg ggcaagcgcc gcctcagtcc aagcgtcggc gccggcgctg gtggatccgc
     1681 ctgtccctgg gcattctgct tctcttgatc atcctggtcc tccttgcccc ctatctggta
     1741 tcgaccgcaa tcggctccag gctgattctg tccctggtca acgatcagat cgaaggtcgc
     1801 gtcgacctcg aaaggctctc tttgtcgtgg ggcggaccga cgaagctcac cgggctgacc
     1861 gtgctggatc cggccggcag agaggtcctg accgtcgagc aagccgtttg gaccggcggc
     1921 gcgtggaccg cattgcgatc ccggcagcag ctcggcgaaa tcagcgtcga ttcaccgcag
     1981 gccgtgctgt acatcgacga gcacaatcga atctcattgg ttcaggcctt cagtcttcgc
     2041 aaaccggccg tcagcacaaa gccaatctcg gttccgccgg taaccggcaa cgtgagtctc
     2101 aataaaggct cggtccgcct ggtgcgggtc gacgggcgaa cgtacgatgt gccccaactg
     2161 aacgcgaagt gtcacgtgga cgccatgcaa gacctgaagg gcacgataga agcaactctc
     2221 gccgacgcct caaccgtggc cttcgacgca agtgtgcggc agtttgtgac gaaaggccaa
     2281 ctggacgtgt ctcaagcatc ggggcagatc agagtcgcca ctggcggcga tgtggacatt
     2341 gccggtctcg cccaattcct cctggatcag gccagcgcgg ccggaagagc acactttgac
     2401 ggcgggctga cctttaaacc aggcgaggcg ctggttgacc ttaagtcgca tgcgacggct
     2461 atccagatca cccgcgccaa cacgccggcg atcaacccca ccgatctggc aatcgaaagc
     2521 cacagccgcc tgacggctga agcgatgtcc gcagatgtgt cgctgaccgg acaggcagga
     2581 aacgcacacg tccgcctggc ttacgcccca tccggccaac cggtccgcat ctcgccgcaa
     2641 cagttgctgt ccgcagcgct gacggggaac ccaatcgcga tgccagactt gaccgccgac
     2701 gcaggtgcca atctgaatct cgccgcactg gcacaggcga taccggccct gctcaccatc
     2761 cggcccgggg tacaaatcac tggcggacag ctcggaatcg aggatttgag cattcgtggc
     2821 ggcgctcagc cggccatgaa aggaacgctc cggctggcgg aactgaccgc tgtcgacaac
     2881 gggcgtacaa cccgctggga accgatcact gcagaattcg acgtgtcact ggaggcaggc
     2941 cagggcctga agatccgcca aacccaggtc gaatcgggct tcgcgcgcgt catcgccggc
     3001 ggcagcgctt cggacctgca cgccgacctc acggcggacc tcgcaaaaat gggccaacag
     3061 ctcggtcagg cagttgacgt cggtgcgaag gtgcccgccg ggtttatcac gggaacgctc
     3121 cgcgtgaagc gagccggcga cgaccgcatg gacttcgcat gcgacctggt cgccagcgac
     3181 ctgcgctaca agacggacaa gggtcaactc gatgctgcga aggcaacact gaagcagtca
     3241 ggtcacctgc aactggccgg cgcagaggtc acgagactcg taacctccag cttcaccgcc
     3301 gacgtcgacg gccaggtcgt cgccaccggt tccgggtggt ttgacctcca gcgcggcact
     3361 tactcggtcg acgcagacgt tgagaacgcc gacctggctt accttggttc caaggcaacc
     3421 gggttcggcg tggaaggtct gaaccgctac gcgggaacgg gtgtcatcca tgttgagatc
     3481 gatcgagcct cgcccgacgg gccaattgtc tcgggcggac aggcgacgtt gcgtaacacc
     3541 acggtggacg gcgagccgct tgccgggcag gacgttgtgc tcgcatggtc ggaagcgagc
     3601 tggtcgccgg aacccggcgc cttgagtgtc aacaccgcag agttcagaag cgacctggca
     3661 cacgcaaccg ccagacaagt cttgctgaag actcgtgagg gactcaccct gaacggcaag
     3721 gtcgaggcga tcgcagattt ggcccagtgc tttgcgacag ccggccgcat cggccagtgg
     3781 aagaccctac cgcgaatcgc cggtcggctc gcgctcaccg gcacctgcgc ttcggcctcg
     3841 ggcaccatcg ccacatcggg caactgccga atcgagtccc tcgagatcgg tgccggcaag
     3901 caggctattc gcgaagacca gcttcagctt gtatacgacg cggccgtaaa cacgcgagaa
     3961 aggaatatca ctctcaagca gtgcgagctt gcctcgagac tgctgtcggc caagatgacg
     4021 ggcaccatcg atcactacgc caccgcgtgc gttctgtcgc tgtcgggctc ctatgaggga
     4081 tcctgggact ccatgaccgc ggtcattcac gagctggtcc cggcaacccg cgactcgctg
     4141 tctctttccg gcaccacggc aggtgcattc accgccaaag gcgcggcgta tcagccggag
     4201 actcggccgg tttatcgcgg cgttgccgcc gggctggatg tcggctggac ctcggtcaag
     4261 gcatacggtg tcaccctggg ccaagcacaa ctctcgccgg tcctgcgcga cggcgaactt
     4321 ttgatcccag tgacggcgat cccggcgagc gtcgggcaag tccgcctcgg cggcctgatc
     4381 gatcttcgca cgtcggaccc gacgtttcgc ctgcccggca gagtccttgt ggtggaagga
     4441 gcccaagtca ccccggaggt cggccggcac gtgctcagca ggatcaatcc cattttcgct
     4501 tttatgaccc gcgccgaggg cacactctca ctggcaacgg aagatatcgt tctgcggctg
     4561 agcgatcaga tcagacaaag cggcagcggc aagggccgac tggatctcaa ggacttcaag
     4621 gtgcagttcg gcgggccgat ggccgtgctg ctcgaattgg gggccatgga cgagcgggac
     4681 atgctcgcgg tgacgatgga cggtgtggac ttcgccctga aagacggccg aatccggtac
     4741 gacaacctca ccatggtgtt ccccgagaac ttcgacctga agttctccgg ctcggttggg
     4801 cttgacgaaa cggtcgatct gaccgtctcc gtacccgtcc gggccgccct tctcgaacga
     4861 ttcggcgtcc gcgggccggt gaccgaatac gcacgcctgc tggaaggtgc tcgcgtggcg
     4921 gttccgatga ccggccatcg gctgctgccg aaggtggacc tgagccgcgt ggatatgaag
     4981 ccattggtcg aacgtgcgat gcgggcagcc gtaacaaggg aagcgcccgg cgtaatagac
     5041 gctctccgca agccaaaaca ggattcgacg gacaaaacgc cgaccaagca accaccgccc
     5101 gcccagccga cgaccaagcc catcgaggac aaagccaaaa ggttgctcga cgaactcctg
     5161 aaagaccgaa agaagaccga caagccagcg ccgaagaaac gataagggca gtcatggtct
     5221 acccggcatg atcagatcgt cccgtcggct gtgaatacgg ttcgccgtcg aacgcagcac
     5281 ccgcgtcgat ggcctcagcc gttgaaatac aaatacgcag cgaggatcac acagaggacc
     5341 agggccgtgc tggcgatatc gcgccagtca tagctgcgcc gtgtctcaag gcggtcgcga
     5401 tccgtgatgg ttccgtaagt aaggccgcga atgcgctcat aatccggcgc tcgcgtaacg
     5461 tagcttacca gtgtcataac cagcgcgctg accacgaaga tgatcacgct gtaatattgg
     5521 aaattcatct tgttgacgat ccagaaaagc gaaccgctcg ggtaaccatt ctcgaaaccc
     5581 gccagcttga gagccacagg ggtgtcaacc gccaagcgga aaacgcccag ggcaaagccg
     5641 acgaccaggg ccgccagaca gccggccgcg ttcaaacggc ggaaaaagac tcccaggaaa
     5701 aagaccgcga aaatcggcgg gcccaggtat gcctgaaccg tctgcagata gtcgtacaaa
     5761 cccttctgtc cgcgcacgac cgggatccag atgaggctga tcaggaccat cgccaccgta
     5821 gccatgcgcc ccacccagac aagctccgcc tgcgaagccc tcggccgaaa cttgctgtag
     5881 aagtcgatgg tgaacagcgt ggaagaggca ttgaagacgc ccgccagcga actcatcagc
     5941 gcggccagca acccggcgac gaccagcccg cgaacgccga cgggcagaat gtgttgcacg
     6001 agcagcggat acgccgcctg ggccttgaaa cggtccaact ggccgttgtt gtacaggccg
     6061 tcgtgcaact cctgcattcc gctcttggcc aaggcgaaac agatcatgcc gggaatgatg
     6121 aacaggaaca ctggcagaag cttcaaaaca ccggcgaaaa tagcaccgcg gcgggcctga
     6181 gtctcgttgg gggcacccaa tgcccgctga acgatgtact ggtccgtaca ccaataccac
     6241 agccccacaa taggggcaca gaacagcatg ccgatccagg gatagttcgt gttgaaatac
     6301 caagcctgcc tgacgacctg accggcagcg tctttgactt cgaccggcga ccaagcggcc
     6361 tcgacgccgg gaggcaccag aggcttccag aggttgaaca tttccgagcc gcagacctcg
     6421 cgcaagtgac cccagccgcc gatggccttg aggccgaaga aggtcaccgc cgccgagccg
     6481 aaaatgagga tgaacgtctg gagggcctcc gtataggcca ccgcacgcat tccgcccaaa
     6541 acggtgtaga tgccggtgag caggacgacc agaatagacc ccacccagaa gctgtccaga
     6601 tgaatgcccc cgatgtccag gcttgcatcg ggcaggaggg cgctgtagac gacaccgccg
     6661 gcgaagatgc cgaccgcgat cttggtgagc acataggcaa acagggatat cagagacaac
     6721 agcagacgat tcttgggcga gaaacgcttc tccaggaact caggcgtggt gaagactctc
     6781 gaacgagcat aaaacggcac gaaaacccac gcgagcacga gtacgcacca tgcgtgcagc
     6841 tcatagtggg ccagagtcac gccgtcggtc gcgcccgagc cggcaaggcc gaccaggtgc
     6901 tcggagccga tgttacaggc gaaaatagag gccccgatga cgaaccagcc caggtgtcgg
     6961 ccggccagga agtaatcgtc ggcggtattc ttagtgcgga ggattaccca ccacgccagg
     7021 cccagcagga tcacgaaata cagagcgatg acaatccaat ctaacagatg catgtaactt
     7081 acctcatttc cagcgttcgt gatgcgccat gcgggcacaa gagtaaacgg cggacaccgg
     7141 ttttggcaac aagtcgaccc ggaccgcggc cctcgcctcc agccaacgat ccttcgagcg
     7201 tcgacgtgct gcagcctgtg actgagaatc actgctcgct ggaaaaacgc gtcggactcc
     7261 tcttcagaga ccacgcggac aaggcgcgac gtcgcactac gacggcgcac atcggtcctt
     7321 gagagtccag acatctgagg gtttatgttc tatgcggccg gcttgcttcc ctcggatctg
     7381 tcaagatcca gccgttcacg gatgaattcc gcgaggaatg aggttttggc ggcgccacag
     7441 acaggcctgg gggccttttg aagtgtgccg gcgtcgtccg ccaacgcggc aaccgtgatc
     7501 tttttcgcat acttgccggg gtggcccctg cggcgtaggc aggtcgccga tggcggggcg
     7561 gccgggaacc ggaccggcgc ctgccggcag agccgctaca cctgccggac gaacagtcgg
     7621 acggaccacg tccgcgagga atgagcgaag gatattcgcc ggtgaatcgc gacccacaac
     7681 ggttcaaacg cctgatcgat cgatggatcg acctggagat cgaacgctca tgactcacca
     7741 tgcagattgg agaaccacca cggagtcggc gggctgaaac tcggtactct cgagaagcgc
     7801 agacacgaaa ggaatatgga ctctctggcg tgcatggggt cgtgaagtgg gacaatcctg
     7861 gggcttggcc ggcttgatgt caccacgtgg ctggagcctg ggatcaccgt gcaccggtgc
     7921 caacatgcct ttcggaaagc gtcgccgcag atcggctcgc tgttgcaaga gctgcgccgg
     7981 gcaccagctt cttctgctga atgtcttgcc gagttttcgg tccagagaag cttgtttgct
     8041 gggctccgat gggtattatg tagagcaaac gctgatggct tgctctactt aagcgggcag
     8101 tcagtcggaa cgtaaggttt tgctagtgga acgtaaggtc ttgctagcaa aagatttggg
     8161 tcgacttgcg ggcgtagctc aattggcaga gcatcagcct tccaagctga atgttgacgg
     8221 ttcgaacccg ttcgcccgct tcgtacataa accacgccgc tcccgatagt tgcgggaagc
     8281 tgccggcgtt cggcagtgcg gcctgaaacg cagaatcgcg gggtgccaca ccgcgctgtg
     8341 cgtttgggag gtcgcgtatg ccgaaaacgt agcctatcgc tctcgtaaag ggacttccca
     8401 agcccttgtt accctgaccg actgggttac gaagaaacgc cgggactact ggctcggtga
     8461 gtacgctacg cccgaaagcg gtgaagccca tcaccgcatc atcgctgact ggacgatcgg
     8521 cggttgtcgc tgacccagcg ccagaggaat acgtcccgaa acccctgcgg ccggcccgac
     8581 gcttatcgag atcgttcagg ccccattggc actggacaca agcgcactat cgcccccggc
     8641 acgcaggggc tctgaagcct gcgcggcgac tactccgaca gttctatgga cacaaacccg
     8701 cggccgattt cggacccacg gatttgcgtc tcctcaggga cgaaacaatt cggggtagcg
     8761 aaaccgcgga tccacctcgc caaccttggt ctcgcaagta caccaacgag caggtcaaga
     8821 ggcttcgaca gttgttcgaa tgggcggcag ctcagcaatt ggcgcctgtg atcgtctatc
     8881 agtcctgcgg cggaccgcct ttttgcgggc cgcaacccga gcgcactgcg atccagaaac
     8941 atgcgcgcaa accgcaccgc tctctggccg taagattctg ctgcgagccc tggcttgcgg
     9001 ggatccgcag ggggctgtgg tgcttcggca atccgggcat gttctttgac aagagcatac
     9061 gcgagattgg cttgccactg gccgggttg
//
LOCUS       DAOTPW010000232         9062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_6984_length_9062_cov_5.033752, whole genome shotgun sequence.
ACCESSION   DAOTPW010000232 DAOTPW010000000
VERSION     DAOTPW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6984_length_9062_cov_5.033752"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            606..1541
                     /locus_tag="P5316_17505"
     CDS             606..1541
                     /locus_tag="P5316_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86807.1"
                     /translation="MRKRSTLLAGLLLVSGIPLVLAPSCTITLVTPEMVTSTETRNVT
                     LPAGASVVVSNELGTTKVQVEETATQATVQIVRVAYAASEEDAEDLLTQMEVTITEPT
                     GGDNRLVVAAKAVEGATSDEGKFNMVVNDDDVVITSVFSRARVAKYRITVTLPPGHGV
                     EVTQKVGLVKAGGLDTASTLDGAAATVVAEGCTAALTANADAGDVKVTSHSGSLNATV
                     SAGSAVIDIAALAADDVVDVAIDAGSIDIDLPAGVAADLDAAASIGSVHFDKTGFTAV
                     TVTTDTLTHVVAKLNGGGAEINLQTDVGNIIIDSR"
     gene            1793..2113
                     /locus_tag="P5316_17510"
     CDS             1793..2113
                     /locus_tag="P5316_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86808.1"
                     /translation="MNWLERFWTGCGHCRGGQECPLAGANSPVGPDGTGGLALRAAVV
                     FLLPLASAIVCSHLAWRMWAGHEPGSSAGWQIAGMAVGIVLGIAVAKVLIRIMRQRQT
                     DEGA"
     gene            2115..3506
                     /gene="rsxC"
                     /locus_tag="P5316_17515"
     CDS             2115..3506
                     /gene="rsxC"
                     /locus_tag="P5316_17515"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HRR86809.1"
                     /translation="MVVAVLRERLSGLKSFARGIHPPHRKEYSETEPIRLFAPKGEIS
                     VPMVQHIGAACTPLVQAKDDVSYGQKLADTDSPVAAPIHASVTGKVAGAGVVLLPSGR
                     RVPALWIKPAEGGQALPADFLSQFLNRDWSGVDPSRYDPDEICRTIRDCGVVGLGGAT
                     FPTHIKLRRNPERPVDTLILNGCECEPYLTSDHRLMVECPEAIVVGLQLAAHAAGARR
                     SIVAIEENKPDAIEAMRKACQGRPIEIAICATKYPMGGERQLVPAVLGRTVPSAPKGL
                     PLDVGVVMVNVATAHSIARAVVKKMPLTHRVVTVTGRGVARPGNWLVPVGTMFSELIE
                     ACGGLTAEAVKVVAGGPMMGPTVPHLNVPVVKGTGGITVMSEEETVRWDESPCIRCGR
                     CVDNCPLHLSPTKIAHAVKFRDYDLANQYDMAACCECGCCSYVCPAHIPLAQYIRAGK
                     NQVRLMASQRKQK"
     gene            3584..4597
                     /locus_tag="P5316_17520"
     CDS             3584..4597
                     /locus_tag="P5316_17520"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRR86810.1"
                     /translation="MSDTTLPAEAAQPKGPDQFLVSAAPHLAAGRTTRGVMFDVVLGL
                     IPLLVAAILFYHARAAWLTIITVLGCLAAEGAANLVRGRSLASLGDGSALVTGMILAF
                     SLPPQLPLYMAFIGGVVAIGLGKAVFGGLGHNLFNPAMVGRAFLMICFPVAMTTWSEP
                     GTLLTVGVDAVTKATPLAAAKFHSDVLPGMWPLFVGKVGGCIGETSALACLIGGGYLL
                     IRRTADYRQPVAMLVAAMVFAAIAHHFAPAKCASALYHLNSGAMLFGALFIVTDYVGA
                     PITPLGRWIFGAGVGVLVILIRVFGGYPEGVMYAVLIMNALTPLIERWTVPTPFGGHV
                     PQG"
     gene            4618..5229
                     /locus_tag="P5316_17525"
     CDS             4618..5229
                     /locus_tag="P5316_17525"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRR86811.1"
                     /translation="MSKFVAESWLILLLGLVFAVLLAGAQTSLQPTIRVNQQKALNEA
                     VGTVVPGTVKTEILPVTGYDREVFRCLDGDGKLIGWAIDAAGMGFADKIRAVIGLSAD
                     AETITGLKVIENVETPGLGNKVAAEPRSESDKVWADQYQGLSAGMETTVVKRTPIKGK
                     NEVQAVTGATISSKAVADLANKAIQRVRPELKKQDRPATAESR"
     gene            5241..5882
                     /gene="rsxE"
                     /locus_tag="P5316_17530"
     CDS             5241..5882
                     /gene="rsxE"
                     /locus_tag="P5316_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HRR86812.1"
                     /translation="MAEENGSNLQQFVDGIIKNNPVLVQLLGMCPTLAVTNSVDNALV
                     MGAAVIFVLVMSNMVTSLFRHLIQPHVRILVFTLTIATFVTIADLVLKAYVFEVSKKL
                     GPFVPLIIVNCIIIARAEVVASRSGLLRSLADALGCGLGFTLALTLLGLVREVLGAGS
                     ITLPGWRILLCDPKVFEPHAWVIMIMPPGAFLTLGVVIGIITQIRMRSARGGA"
     gene            5882..6499
                     /locus_tag="P5316_17535"
     CDS             5882..6499
                     /locus_tag="P5316_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRR86813.1"
                     /translation="MDIGVLIGIFIGAALVNNFVLSTFLGICPFLGVSRKVDMAFGMG
                     CAVTFVMTISGIVTWLIVHGILTPIGAWLGNPESLYFLKYVTFIMVIASMVQLVEMYL
                     RKFFPSLYTAFGVFLPLITTNCAILGACLLIDMNEIYRASLLKSTVFAFAGGIGFMMA
                     ITIMAGIREHLRFSDVPKALRGPAITLLTACILSLAFMGFAGMGK"
     gene            6515..7408
                     /locus_tag="P5316_17540"
     CDS             6515..7408
                     /locus_tag="P5316_17540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HRR86814.1"
                     /translation="MNVVLIAGVVLFGLTLVFAMLLGIAKEKLRVEEDPRIPAVLDVL
                     PAANCGGCGFAGCADFAKAVVEKRAPVDGCTVGGSKTAQAVARVLGVEVVETFPYRPV
                     VHCGAKTEDKLGRVPYDGVASCVEANVVGVTQACTYGCLGFGDCAKACPFDALHMIEE
                     HPVVDYEKCTGCGACVRACPRNIIRMVPFKQDRMLVVACSNKEPARSVRQVCKVGCMG
                     CKACQRNFAQLFEVRDNLSYLNYDNYTGEEDFEKAINKCPSGVMMFFGKPKPEYAEIL
                     AQDEAVGGRSEGGEPACSAKV"
     gene            complement(7398..8417)
                     /locus_tag="P5316_17545"
     CDS             complement(7398..8417)
                     /locus_tag="P5316_17545"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRR86815.1"
                     /translation="MNSPSSRSTAAFLACIVVCTTGCSRRPQPSTAKQAREVMGTLAE
                     VTAVAADRTTAQTAVEAAYAAFEQVNSLMSDYRDDTEISRLNSLPPGESLVVSPETFF
                     VLQRAAEVSQASGGVFDITCRPLVALWKQAGQNNRLPDPSAISDVLAAVGWTKLALDP
                     PTRGVTKGVAGMQIDVGGIAKGYALDLAARRMKAAGATSGLIDVGGDIVAMGRQANGD
                     PWRIGVQHPFQSGLIMKLALSDRAVATSGNQQRFTVIGGQRYSHIIDPRNGRPAEQAP
                     SVTVIAPDGISADAWATVFSVLSVEQGARKAEELPDVEVMWIAGDATQPQIAKTPGFG
                     KYIIP"
     gene            8475..>9062
                     /locus_tag="P5316_17550"
     CDS             8475..>9062
                     /locus_tag="P5316_17550"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRR86816.1"
                     /translation="MGDMAAPVNEIRTGRLPPWLKRPRPDAAMLATRAVVEAGGVATV
                     CREARCPNVTECWSRRTATFMILGSTCTRSCRFCAVSRGRPLPPAGDEPQRLAEAVAG
                     LGLRHVVITSVSRDDLEDEGASHFAACVREVRARLPESTIEVLPPDFHAREECILALC
                     QARPDVYNHNIETVERLTPQVRPQADYRRSLRVLRL"
BASE COUNT         1532 a         2441 c         3209 g         1880 t
ORIGIN      
        1 gggaccgagg cctcaagggc gagcatcagc acgccgcaaa aatgccaaac tccagccatg
       61 cccacggctt tgcgtgggca tgcgcctttc aatccttacg gccggacgtt tgtgcggctt
      121 gcgcgcacgc ttcgggatcg cagaacgttt gggttgcggc ccgcccaagg gcggtccgcc
      181 ttaggtggcg cgtaagcggg acgctgcgga tgctgcaggg cgctgggcgg gagcgagctg
      241 aggtgcagcg ccgtttctga gggatctgcc tcagagtctg tgcgaaaaac gacggagctg
      301 atcgctgcgg cagggccgag gtggtggtgg cgaccgagac ctaccatatt ctgcaggcca
      361 agattggcag gttttcgtcc tgctccgtcc ttgtgacttc gcagggctta gaccatgccc
      421 tgccattttc ccgcacatcc gcaggtttat atcgctctga acctggctac tgtcggtcct
      481 ccgtcagtgg cagtttggtg agctttttag gttctgcgga cacatgaggg gtcatagaat
      541 ttggggtatt taccgttctg gtggtcggag tttcatttcc gagacaaagc ttgggaggac
      601 acgtcatgcg gaagcgttca acgttgcttg caggattgtt gctggtttca ggaatcccac
      661 ttgtattggc gccgagttgc accatcactt tggtcacacc ggagatggtg acatcgacgg
      721 agacgcggaa cgtaacgctg ccggccggcg catccgtcgt tgtatccaac gaacttggca
      781 cgaccaaggt gcaggtggag gagacggcaa cgcaagccac ggtgcaaatc gttcgtgtgg
      841 cctacgcggc cagcgaagag gatgcagagg acctgttgac ccagatggag gtgaccatca
      901 ccgagcccac cggcggggac aatcgtctgg ttgtcgcggc caaggcggtg gaaggggcga
      961 cgagcgacga gggcaagttc aacatggtcg tcaacgacga tgacgtggtg atcacgagcg
     1021 ttttctcgcg tgcccgagtg gccaagtaca gaatcacagt gactcttcca cccggccacg
     1081 gtgtggaggt gactcagaaa gtcggcctgg tgaaggccgg cggtctggac acggccagca
     1141 cgctggatgg tgcggccgca acggtggtgg ccgaaggctg cacggcggcc ctgacggcca
     1201 atgccgatgc cggcgatgtg aaggtgacct cgcatagcgg gtcattgaat gcgacggtat
     1261 cggcggggtc ggcggtgatc gatattgcgg ctctggcggc tgatgatgtg gtggacgtgg
     1321 caattgacgc aggatccatt gacatcgatc tgccggccgg cgtggctgcc gatctggacg
     1381 cagccgcaag catcggatcg gtccactttg ataagaccgg cttcacggct gttacagtca
     1441 cgaccgacac gctgacgcac gtggttgcca agctgaacgg cgggggtgcg gagatcaatc
     1501 tgcaaactga cgtcggcaac atcatcatcg actcccgatg acacgtctgg ccggatcggc
     1561 atcgacgtgg tgtccgtctg cttgctgatt gccggtgagc tgaatcggac acactggtat
     1621 cgtatcgggg catgggcgat ccgcttgccg cccatgccct tttccttgtt cgcggcttga
     1681 gccgatattc agggggacgg tcttgcagtc ggcagacaag cttcgataat actgtgcttt
     1741 aacgcttgga tggcggcaga ggcggcgcgg ctatgctgct gagggatcag cgttgaattg
     1801 gctggaaagg ttctggacag gttgcggcca ctgtaggggc gggcaggagt gtccgctcgc
     1861 gggtgcgaac agcccggtcg ggcccgatgg cacgggcggc ttggcactgc gggcggcggt
     1921 ggtctttctg ttgccgttgg catcggccat tgtctgttct catctggcgt ggcggatgtg
     1981 ggccggccat gagcccgggt cgagcgccgg ctggcagatt gccggcatgg ctgtcgggat
     2041 tgttctgggg atcgctgtgg caaaggtgtt gatccgaatc atgcggcagc ggcagacaga
     2101 cgaaggagcg tgagatggta gtggccgtgt tgcgggagcg gctgtcagga ctcaagagtt
     2161 tcgcgagggg catccatcct ccgcatcgca aggaatactc ggagacagaa ccgatccgtt
     2221 tgttcgcacc gaagggcgag atctctgtgc cgatggttca gcacatcggc gcggcctgca
     2281 ctccgttggt ccaggcgaaa gacgatgtga gctacggtca gaagctggcg gacacggatt
     2341 caccggtggc ggcgccgatt cacgcgagcg tgaccgggaa ggtcgcgggg gcaggggtgg
     2401 ttcttcttcc cagcgggcgt cgcgtgccgg cgctgtggat caagccggcg gaaggcggtc
     2461 aggcgcttcc ggcggatttc ctgtcgcagt tcctgaaccg cgactggagc ggcgtggacc
     2521 cgtcgcggta cgatcctgat gagatttgcc ggacgatccg ggactgcggg gtcgtaggct
     2581 tgggcggagc cacatttccg acgcacatca agttgcggcg aaatccggaa cgtccggtcg
     2641 atacgctgat tctcaacggc tgcgagtgtg aaccatacct gacttccgat catcgcttga
     2701 tggtcgagtg tccggaggcg atcgtggtcg gtttgcaact ggcggctcat gcggcgggtg
     2761 cgcggcggtc gattgttgcg atcgaggaga acaagcctga tgcaatcgag gcgatgcgca
     2821 aggcctgcca ggggcggccg attgagatcg cgatttgtgc cacgaagtat cctatgggcg
     2881 gcgagcgtca attggtgccc gcggtgctgg ggcgcactgt tcccagtgct ccgaaggggc
     2941 tgcctctcga cgtcggcgta gtgatggtta acgtggcgac ggcccacagt atcgcgcggg
     3001 cggtcgtgaa gaagatgccg ctgactcacc gggtcgtcac agtaaccggc agaggggttg
     3061 cacggccggg caactggctg gtgccggtgg ggacgatgtt ttcggagctg atagaggctt
     3121 gtggcgggtt gacggctgag gcggttaagg tggtcgcggg tggtccgatg atggggccga
     3181 cggtgccgca tttgaacgtg cccgtggtca aggggacggg cgggatcacg gtgatgagcg
     3241 aggaggagac tgttcggtgg gacgagagtc cctgcatccg gtgcggccgg tgcgtggaca
     3301 actgtccgct gcatctctcg ccgacgaaga tcgctcacgc ggtcaagttc cgtgattacg
     3361 atctggccaa tcagtatgac atggcggcct gctgtgagtg cgggtgctgt tcatacgtgt
     3421 gtccggctca cataccgctg gcgcagtaca tccgggccgg caagaaccag gttcgactga
     3481 tggcaagtca gaggaagcag aaatgagagg agagcccagc acgggtcttg cgaggcggtc
     3541 gggcccgtga tcgtcattca cagggccagg agcacgagtt cagatgagtg atacaacttt
     3601 accggccgag gctgcccagc ctaaggggcc ggaccagttt ttggtcagtg cggcgccgca
     3661 cttggcggcg gggcggacca cgcggggcgt gatgtttgac gtcgtgttgg ggcttatccc
     3721 gcttctggtt gccgcgattc tgttctatca cgctcgggct gcttggctga cgatcatcac
     3781 cgttctgggt tgcctggcgg cggagggagc ggcgaatctg gtccgcggac gcagcctggc
     3841 ttcactcggc gacgggtcgg cgttggtaac gggcatgatt ttggcgttct cgctgccgcc
     3901 ccagttgcct ctgtacatgg cgttcatcgg cggcgtggtc gcgatcggcc tggggaaagc
     3961 cgtttttggc ggcctggggc acaacctctt caatcccgcg atggtggggc gggcgtttct
     4021 gatgatttgc tttcccgtgg cgatgaccac gtggtctgag ccggggacat tgcttaccgt
     4081 cggagttgac gcggttacca aggcgacgcc gctggccgcg gccaagttcc acagcgacgt
     4141 gttgccgggc atgtggccgt tgttcgtggg gaaggttggc ggctgcatcg gtgagacgag
     4201 tgccctggcc tgtctgatcg gcgggggata tcttctcatt cgccgcaccg ccgattaccg
     4261 ccagccggtc gcgatgctgg ttgcggcgat ggtttttgcg gccattgccc atcactttgc
     4321 accggccaag tgcgcttcgg ccctgtacca cctgaacagc ggggcgatgc ttttcggagc
     4381 cctgttcatc gtgacggact acgttggggc gccgatcacg ccgctggggc gatggatctt
     4441 cggagcgggc gtgggcgttc tggtcattct gatccgggtg ttcggcggat accccgaagg
     4501 tgtgatgtac gcggttctga tcatgaacgc attgacgccg ttgattgagc gctggaccgt
     4561 gccgacgccg ttcggcgggc atgtgccgca gggttgagcg gaaggagaag gcgatcgatg
     4621 agcaagttcg tcgccgagtc gtggctgatt ctgttgctgg ggctggtgtt cgcggtgctg
     4681 ttggcggggg cacagacgag tttgcagccg acgattcggg tcaatcagca gaaggctctg
     4741 aacgaggccg tcggcacggt ggttcccggg acggtcaaga ccgagatact gcccgtgacc
     4801 ggctatgatc gagaagtctt cagatgcctg gacggcgacg gcaagctgat cggctgggcg
     4861 attgacgcgg cgggcatggg tttcgcggac aagatcaggg cggtgatcgg cctgtcggcg
     4921 gatgccgaga cgatcacggg tctgaaggtg atcgaaaatg tggagacgcc ggggctgggc
     4981 aacaaggtcg ccgccgagcc gagaagcgag tcggacaagg tctgggcgga tcagtaccag
     5041 ggtctctccg cgggaatgga gaccacggtg gtcaagcgga ccccgatcaa aggcaagaac
     5101 gaggtacagg cggtgaccgg ggcgacgatc tcgagcaagg cggtggcgga ccttgccaac
     5161 aaggccattc agcgcgtgcg gcccgagttg aaaaagcagg atcgtcccgc cacggcggag
     5221 agtcgttgag gagcaggtcg atggccgaag agaacggatc gaaccttcag cagtttgtgg
     5281 acgggatcat caagaacaat cccgtcctgg ttcaactgct gggcatgtgt ccgacgctgg
     5341 cggtgaccaa ttcggtggac aatgccctgg ttatgggggc ggcggttatt ttcgttctgg
     5401 tgatgtcgaa catggttacc agcctgttcc gtcatctgat tcagccgcac gtgcgcatcc
     5461 tggttttcac gctgacgata gcgacatttg tgacgatcgc ggatctggtg ctcaaggcgt
     5521 acgtcttcga ggtcagcaag aaactcggtc cttttgtccc gctgatcatc gtcaactgca
     5581 tcatcattgc ccgggcggag gtggttgcgt ccaggagcgg gttgctgcgg tcgctggccg
     5641 atgccctcgg gtgcggtctg gggttcacgc tggcgttgac gcttctgggg ttggtgcgcg
     5701 aggtgctggg tgcgggtagc atcacgctgc cgggctggcg gattctgctt tgcgatccga
     5761 aggtgttcga gccgcacgca tgggtgatca tgatcatgcc gcccggggcg tttctgactc
     5821 tcggagtggt gatcggaatc atcacgcaga tccggatgcg gtcggcgaga gggggtgcct
     5881 gatggacatc ggcgtactca tcggcatctt catcggggcg gccctggtca acaatttcgt
     5941 gttgtcgacc ttccttggga tctgtccttt tctgggtgta tctcgaaagg tggacatggc
     6001 cttcggcatg ggctgcgcgg tcacgtttgt gatgaccatt tcggggattg tgacgtggct
     6061 gatcgtccac gggattctca cgccgatcgg ggcgtggctg ggcaatcccg agtcgttgta
     6121 cttcctgaag tacgtgacgt tcatcatggt catcgcgtcg atggtgcaac tggtggagat
     6181 gtatctgcgc aagttctttc cgagtctcta cacggccttc ggcgtgttcc tgccgctgat
     6241 cacgaccaac tgtgcgattc tcggggcgtg tctgttgatt gacatgaatg aaatctaccg
     6301 ggcgagtctg ttgaaatcga cggtgttcgc ttttgccggc ggtatcggct tcatgatggc
     6361 gatcaccatc atggccggca ttcgcgagca cctgcggttc agcgacgtgc cgaaggctct
     6421 gcggggcccc gctattacgc tgctgaccgc atgcatcttg tcgctggcgt tcatgggctt
     6481 tgcggggatg ggcaagtaag ctggagatgg tgcgatgaac gtggtcctga tcgccggggt
     6541 tgtcctgttc ggcctgacgc tggtatttgc gatgctgctg ggcatcgcga aggagaagct
     6601 gcgggtcgag gaggatccgc ggataccggc cgtgctggac gtgcttccgg ccgccaactg
     6661 cggcggatgc ggatttgcgg gctgcgccga ttttgccaag gcggtggtcg agaagcgggc
     6721 gcccgttgac ggctgcacgg tcggaggatc gaagaccgcc caggccgtgg ctcgcgttct
     6781 cggcgtcgag gtcgttgaga cctttccgta tcgcccggtg gttcactgcg gcgccaagac
     6841 tgaagacaag ctgggccggg tgccctacga cggcgtcgcc agttgcgtgg aagcgaacgt
     6901 agtcggcgta acgcaggctt gcacgtacgg ttgccttggg ttcggcgact gcgcgaaggc
     6961 ctgccccttc gatgccctgc acatgatcga ggagcatccg gtggttgatt atgagaaatg
     7021 caccggttgc ggggcctgcg tgcgggcgtg tcctcgcaac attatccgca tggtgccgtt
     7081 caagcaggat cggatgcttg tggttgcctg ttcgaacaag gagcctgctc gcagcgtgag
     7141 gcaggtgtgc aaggtcggtt gtatggggtg caaggcatgt cagcggaact tcgcgcagct
     7201 tttcgaggtc cgcgacaacc tctcatatct gaactacgat aactacacgg gggaggagga
     7261 cttcgaaaag gcgatcaaca agtgtccgtc gggcgtcatg atgtttttcg gcaagcccaa
     7321 gcccgaatac gcggagatac tggcccagga tgaagccgtc ggcggccgat ctgagggcgg
     7381 cgaacccgca tgctcggcta aggtatgatg tatttgccga aaccgggcgt cttggcgatt
     7441 tgaggttgtg ttgcgtcgcc ggcgatccac atgacctcga cgtccggaag ttcttcggcc
     7501 tttcttgctc cctgctcaac cgacagcacg ctgaagaccg tggcccacgc gtcggcgctg
     7561 atgccgtccg gggcgatgac ggtgacgctg ggggcctgtt cggccggccg gccgttgcgc
     7621 gggtcaatga tgtgagaata ccgttggcct ccgatgaccg tgaatcgctg ctggttgccg
     7681 gaggtggcga cggctcggtc gctcagggcc agcttcatga tcaggccgga ttggaaggga
     7741 tgctggaccc cgattcgcca ggggtctccg tttgcctgtc tgcccatggc gacgatatcg
     7801 ccgccgacgt cgatcaagcc gctggttgct cccgcggctt tcatgcgccg ggcggccagg
     7861 tcgagggcat agcctttggc gatacccccg acatcgatct gcataccggc cacgcccttg
     7921 gtgacgccgc gggttggggg gtcaagagcc agcttggtcc agccgacggc cgcaagaaca
     7981 tctgagatcg cggacgggtc tggaagccgg ttgttctggc ctgcctgttt ccacaaggcg
     8041 accagcggcc ggcaggtgat gtcgaaaacc ccgccactgg cctgtgacac ctcggcggcg
     8101 cgctgaagta cgaaaaaggt ttccggtgag acaacgagcg attcgccggg cggcagggag
     8161 ttgagccgcg agatctcggt gtcgtcgcga taatcgctca taagtgagtt gacttgctcg
     8221 aacgcggcat aggcggcctc gacggccgtt tgtgcggttg tccgatcggc ggcgacggcc
     8281 gtgacttcgg cgagtgtgcc cataacctcg cgagcctgtt tggcggtaga cggctggggt
     8341 cgcctgctgc aacctgtcgt gcagacgacg atgcaggcga gaaacgctgc tgtgctgcgt
     8401 gatgatggag aattcatggc cggaatcgtc gcatacgccg gctcggcttg caaacgtgca
     8461 tgccaggagg ttgaatgggc gacatggctg ctccggtcaa tgagattcgc accggccgac
     8521 taccgccttg gctgaaacgt ccgaggccgg atgcggccat gctggccacg cgtgcggtag
     8581 tcgaggccgg cggtgtggcc accgtttgcc gggaggcccg ttgcccgaat gtgaccgagt
     8641 gctggtcgcg acggaccgct acgttcatga ttctgggcag tacctgcacg cggagttgca
     8701 ggttttgtgc ggtgagtcgc ggtcggccct tgccgccggc cggggatgag ccgcagcgtc
     8761 tggccgaggc tgtcgccggg cttggtctgc ggcacgtggt catcaccagc gtgagccgcg
     8821 acgatttgga ggacgagggg gcgagccatt ttgccgcctg cgtccgcgag gttcgtgccc
     8881 gcctgccgga gtcgaccatc gaagtgctgc cgcccgattt ccatgctcgt gaagagtgca
     8941 tcctggcatt gtgccaagcc cggcccgacg tatacaacca caacatcgag acggtggaac
     9001 ggttgacgcc gcaggttcgc cctcaggccg actatcggcg ctcgctgaga gtgctgcgac
     9061 tg
//
LOCUS       DAOTPW010000233         9030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7014_length_9030_cov_5.468189, whole genome shotgun sequence.
ACCESSION   DAOTPW010000233 DAOTPW010000000
VERSION     DAOTPW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_9030_cov_5.468189"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            994..2736
                     /locus_tag="P5316_17555"
     CDS             994..2736
                     /locus_tag="P5316_17555"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRR86817.1"
                     /translation="MSRSVVLSFAMIGASYAYAEADVKVWTRLVGSTNYDLGYAVAVD
                     PAGNCIIAGATQGSLAGSNAGRYDLFAAKYDPSGNRVWLAQRGTAEREFAYGVATDAT
                     GNVYVTGYTGSGLDGNKSLGEWDIFLMKFGPTGNWLWTRQDGTGPDDEGRAVATDSSG
                     NVYVTGYVRGNFHGIARVGSADVFISKYDPSGNRLWSALFGSTEVDESFGITCDASGN
                     VFVTGWCSGSIEGNPYLGNGDNFLVKYNSSGQRQWLRVWGTANKDTGYSVACDAAGNV
                     YVSGYSAGPLYASPEGNRDYFLAKYDASGNLLWGRQTGTAGHDQGWGVATDLAGNVYV
                     AGETGGPLDGNVHQGGLDVFLSKYDAAGTRLWTAELGTAGEDWADGVAVAGNGDVFLG
                     GTTTGNLDGNANQGLQDAFVMRFSPAASVLPAEPTDAVATPATIYRGCTSSQLSATPG
                     SGGDTVEWFTVSCGGTEVTGGASPMVSPSVTTEYYARTKDSVTGLTSSTCATVVVTVN
                     LPLPADLDQDCDVDRSDFNIFTACLSGSSVPWGSGCDGSDLDEDGDVDQLDFGMFQRC
                     YSGADKLFDPNCAD"
     gene            complement(2809..4362)
                     /locus_tag="P5316_17560"
     CDS             complement(2809..4362)
                     /locus_tag="P5316_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRR86818.1"
                     /translation="MPVLLDVWREACKHIEIGESVSRIGPVLTRRVPVDLVLVRRLDA
                     PRSAVETVAAGVCGTAAAPEQTRNECSPDEFRRLLAWCREGKVLRDDATTIRQIHPAL
                     LPAGVEGQVMVGSLNREDEPVGVLVLATQRRSGLRREHEELATALLEPFAVALENDRQ
                     LRELSVLREAVEADNRSLLARLGRQDISDSVVGSETGLKEVMERVNLVAHSDVPVLIL
                     GETGSGKEVVARAIHKGSPRSAGPFLRVNCGAIPPELIDSELFGHERGSFTGAVATRK
                     GWFERADGGTLFLDEVGELPLAAQVRLLRILQDGSFERVGGQRPLHVDVRLVAATNRD
                     LHAMVAEGTFREDLWYRIAVFVVRIPPLRERIEDIPMLAGHFALRAAKRLGTPPLVPT
                     PEDNDLLVSYDWPGNVRELSAVIERAAILGMGRRLEVAAALGVNTRIAPRQANHTRLL
                     PTVQAHDNDAVITLDAAMAQHIKRALQRTHGRIEGPYGAAKLLGINPYTLRARMRKLG
                     IDWASFRTT"
     gene            4682..5596
                     /locus_tag="P5316_17565"
     CDS             4682..5596
                     /locus_tag="P5316_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRR86819.1"
                     /translation="MADNALVGKSIQAAGVPRRFWLLGLGMAGVLGFLAVHTLYPEWH
                     LLPQATSQYRPATLLAVFLAAFICEYIDSALGMGYGTTLSPMLLMFGFDPHAVVPCVL
                     ISELITGVTATLMHHRDGNVDFLRDKNARGTAILLSVLSVVGAVIAVRLANKLSVPGL
                     RLAIATVILATGVFTLATVRRQLRYRKSHIITVGAIAAFNKGLSGGGYGPLVTAGQVV
                     SGLSPKHAVAISSLAESFVCLVAIIASLWLGKDLNWGLALPLAVGAVMSVPLATMTVR
                     WLPEKAMRASVGVMTIVLAIVMLTGGKA"
     gene            complement(5744..6364)
                     /locus_tag="P5316_17570"
     CDS             complement(5744..6364)
                     /locus_tag="P5316_17570"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HRR86820.1"
                     /translation="MHTFAKRIGIGACSSVCVLAITLGLLLGGSSVGRGFAPRPHRET
                     GTLRVLVKNSPCLAERVGTTRLTVSRIEVRHADNHDAWVVIRDGEQVIDLLQTNEGRP
                     CVLASADLAPGRYSQVRLICPEESIAVKDGHTPDWKKARSRNTPYGTQTAIHLNCDFA
                     VSADDQTAILIDTGLEDALRMIAQDRRTDVEQVAGFRTAPPASPTD"
     gene            complement(6601..8577)
                     /locus_tag="P5316_17575"
     CDS             complement(6601..8577)
                     /locus_tag="P5316_17575"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR86821.1"
                     /translation="MMRKCTLLGLLTLAATACLLNTTAAAPASDLEKQYIDKITEWIP
                     GMSDPDIPKRKDPQQALEKLCHEAGAPGKAAEREALCRAMMTKVGPDVPKPARIWILR
                     KVEPLGRDEVVARLTELLRDEDADIRETARRALVNNPSPQAGASLRAELARADTSKWQ
                     IAMINGLAFRRDGQAVDAIAKLTSSPDDAVATAAVCALGDIRTPEAIKAVTALLEKPR
                     PTLHASIAEAAMKCGEELIAKGSADQAAAIYERLYDKSRPESIRIAGFQGIAAARGTK
                     ALPMLFDVLNGDDARMRMVAARSIQEIPGDEVTRKLLAALGNAQPETAELIIDVLGQR
                     GPEARQAVARLAREAKEPAVKDAAVLALAQLGDASAMDALLLIAKSSENATHRSVALR
                     GYVRLAREQGGPEERLQTLTTAMSLANNADDKKLIVSAIGDIPSVKALDELMSLVDGD
                     LHAEAFAAAVSVSKRIAGRNHAAALDAVDKLSRLTKTDSEKNAIDALRDALTSYCTSW
                     MVSGPYKQKGKEGLETFDVVFPPEEPQGNVKEWKPLNVTNPDHPGHFDLGKGNNCCAY
                     VRTTVISESEREVQMAFGSDDAIKVWLNGQLIHANKVNRPCACDQDKVKATLKKGENT
                     LLIKVVQGGGDWAFCCAIRDPDGKPLTGISYKAE"
     gene            complement(8597..>9030)
                     /locus_tag="P5316_17580"
     CDS             complement(8597..>9030)
                     /locus_tag="P5316_17580"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86822.1"
                     /translation="VEIIPPNGKDVEHMTFKYANGVIMQRAQANGVLFSGTEGDIEVN
                     RGYFQAEPKSIAEEPLGPNDVHLYKSPGHRQDWINCIRSRKRPICDVAIGASSVTVCH
                     LSNIAYWLGRPIKWDPEKREIIGDSEASRWMDRPKRAPWRL"
BASE COUNT         1528 a         2560 c         3087 g         1855 t
ORIGIN      
        1 gggccgagcg agcgagagtg tggcccggaa aggattccgg gccgagcgag cgagagtgtg
       61 gcccggaagg gattccgggc cgagcgagcg agaacgtggc ccggaaagga ttccgggccg
      121 agcggttcgg ttggaggcgc atgcccacgc agagccgtgg gcatggcacc cggttggcct
      181 gccctacctg cttattattg actggcactg ttgagcgaga ccgacgctga tcacgccaag
      241 gttgtttctc tcaacgagcg acttgtcgat ccgatgctga cgacggcttg gacgctgacc
      301 gaagttggcg gcgctctcgc atcgtacggt gttgcccgaa cggcacccaa atgctaggat
      361 aaacagcgtg tcggggcgct ccggatcggc agcgggacgg tttgcggtct tgtgaggtag
      421 cagcgtctcg gcgacagcag agcggcgggg cggccgttga aggcaagcct ggagtttggg
      481 gaggcttggc gtggtagaca aggagatact ggaggttgag aggccgcggc cgttcccagg
      541 gctgcgaata catgtctcgg atgggggcgc acctaggaag tcaggcatcc ggagatgatg
      601 ctggtgacgc ggatcctggt cgtcgtcgcg ccggctgaag gtgaggaggt tccgcagcgc
      661 aggggtctat tgcgacccgg tgcacgttac ccggatcgag ccgattagca caaccttgaa
      721 ggcttcgtga ctttgtgcgt gttcctgtgc gaggagtacg ctgatggatt gcagatctcg
      781 aattgcagag tgtagaacat gggtcgagat cgcagattcc acctggatta cgcatagtcg
      841 ttgggtcatc tgaccacgga ttgcacggat atcacggatc ggagcaggac tgggaagatg
      901 gtgcgcgggc gtagaccctg cggcgggtga tggcgggcgg ccggcgttga ccgtggcttg
      961 ttggatttca tgaaaacgag gagtgcgggt acggtgagca gatcagtcgt tttgagtttc
     1021 gcaatgatcg gagcgagtta tgcctacgcg gaggccgacg tgaaggtctg gacccgtctg
     1081 gtggggagca cgaattatga cctgggatac gcggtggccg tggaccccgc gggcaactgc
     1141 atcatcgcag gggcgaccca gggttcgctg gcgggctcga acgcggggcg ctacgacctg
     1201 ttcgcggcga agtatgaccc gtcgggcaat cgcgtgtggc tggcccagcg aggaaccgcc
     1261 gaacgggaat tcgcttacgg ggtcgcgacg gatgccaccg gcaacgtcta cgtgacgggg
     1321 tacaccggca gcggcctcga cgggaacaag agcctggggg agtgggacat tttcctgatg
     1381 aaattcggac cgaccggcaa ctggctgtgg accaggcagg atgggaccgg cccggacgac
     1441 gagggtcgtg ccgtggccac ggattcgtcg ggcaacgtgt atgtcaccgg ctacgtgagg
     1501 ggcaattttc acggaatcgc gcgggtgggg tcggcagacg ttttcatcag caagtatgac
     1561 ccgtccggca accggttgtg gtccgcgctg ttcggctcga cggaagtcga tgagagtttc
     1621 gggatcacgt gcgatgcctc cggcaacgtc ttcgtcacgg ggtggtgcag cgggagcatt
     1681 gaaggcaatc cttatctggg caacggcgac aatttcctgg tgaagtacaa cagcagcggg
     1741 cagaggcagt ggttgcgggt gtggggcacg gccaacaagg atacgggtta ttcagtggcc
     1801 tgtgacgcgg ccggcaacgt ctacgtttcc ggctactcgg cggggccgct ttatgcttcg
     1861 ccggagggca accgcgatta cttcctggcc aagtatgatg cgtcggggaa tctgttgtgg
     1921 ggaagacaga cgggcacggc ggggcacgat cagggttggg gcgtggcgac ggaccttgcc
     1981 ggcaacgtct atgtggccgg agaaacgggc gggccgcttg atggaaacgt gcatcagggc
     2041 gggctggacg tttttctgag caagtatgat gccgcgggaa cgcgtctgtg gacggccgag
     2101 cttggcaccg ccggcgagga ttgggccgat ggcgtcgcgg tcgccggcaa cggggacgtc
     2161 tttctcggcg gcacgacgac ggggaacctt gatggcaacg cgaaccaggg gcttcaggac
     2221 gcgtttgtca tgagattcag tcctgccgcg agcgtgctcc cggccgagcc gaccgacgcc
     2281 gtggcgaccc ccgcgacgat ctacaggggc tgcacctcat cccagctttc cgcgactccg
     2341 ggcagcggcg gcgacaccgt cgagtggttc accgtcagtt gcgggggtac ggaggtgacc
     2401 gggggtgctt caccgatggt cagcccgagc gttaccaccg agtattacgc aagaaccaag
     2461 gactcggtta caggcctgac gagcagcacg tgcgcgaccg ttgttgttac ggtcaacctg
     2521 cctcttccgg cggacctcga ccaagactgc gacgtggatc gatcagactt caacattttc
     2581 actgcctgcc tgtcgggttc gtcggtaccc tggggttcgg ggtgcgacgg cagtgacctt
     2641 gacgaagacg gcgacgtgga tcaattggat tttggcatgt tccaacgctg ctacagcggg
     2701 gcagacaagc tgttcgatcc caactgtgcg gattgacatg gacggcagga tgccggttcc
     2761 gcaccattgt tgacgggcaa gatggcggcc ccgcggaacg gcccgcgttc acgtggtccg
     2821 gaagctcgcc cagtcgatgc ccagttttct catgcgtgcg cgaagagtgt aggggttgat
     2881 tcccagcagt tttgcggcgc cgtaggggcc ttcgatgcgg ccgtgggttc gctgtaaggc
     2941 ccgtttgatg tgttgggcca tggctgcgtc gagagtgatc accgcgtcat tgtcgtgggc
     3001 ctgaacggtc gggagcaatc gggtgtggtt tgcctggcgg ggggcaattc gcgtgttgac
     3061 gccaagtgcc gcagcgactt cgaggcgtcg tcccattccg agaatggccg cccgctcgat
     3121 gaccgccgac aattcgcgta cgttccccgg ccaatcgtat gagacgagca aatcgttatc
     3181 ctcgggggta gggaccagcg gcggcgtgcc gagtcgcttg gcggcgcgga gggcaaagtg
     3241 gccggccagc atggggatat cctcgattcg ctcccgcagc ggcgggatgc gaaccacgaa
     3301 gacggcgatg cggtaccaga ggtcttcgcg gaaagtgccc tcggcgacca tggcatggag
     3361 atcgcggttg gtggcggcca ccaggcggac gtccacgtgg agcggccgct gtcccccgac
     3421 ccgttcgaat gagccgtcct gaagaatgcg aagcaagcgg acctgggcgg ccagcgggag
     3481 ttcgccgacc tcgtcgagga acagcgtgcc gccgtcggcc cgctcgaacc agcctttgcg
     3541 cgtcgccacc gctccggtga agctgcctcg ctcgtgaccg aacagctcgg aatcgatcag
     3601 ttcggggggt atggctccgc agttgactcg caggaacggt ccggcacttc gcggcgagcc
     3661 cttgtggatg gcccgggcga cgacttcctt gcccgagccc gtctcgccga ggatgagcac
     3721 ggggacgtcc gaatgggcaa cgaggttgac gcgttccatc acttccttca ggccggtctc
     3781 tgaacccacc accgagtcgc tgatgtcctg gcgcccgagc cgggccagga gcgagcggtt
     3841 gtcggcctct acggcctcgc gcagtacgct caactcccgc aactggcggt cgttttcgag
     3901 ggccacggca aaaggttcca gaagcgcggt tgccaattcc tcatgttcgc gccgcaagcc
     3961 ggagcggcgc tgcgttgcga gcacgagcac gccgacgggt tcatcttcgc ggttcaggga
     4021 gccgaccatg acctgtcctt cgacccctgc gggcagcaag gcggggtgga tttgtcgaat
     4081 cgtcgtggcg tcgtcgcgga ggaccttgcc ttcccggcac caagccaaca gtcgcctgaa
     4141 ctcgtcgggg ctgcactcgt tgcgtgtttg ttcgggggcg gctgcggtgc cgcagacgcc
     4201 agccgcgacc gtttccacgg ccgatcgggg ggcgtcgagc cgtcgcacca gaaccaagtc
     4261 gaccggcact ctgcgggtga ggaccggccc tattcgggag actgattcac caatctcgat
     4321 gtgcttgcag gcttcccgcc aaacatccaa caaaacgggc atgaatcgtc tcatgagcaa
     4381 taccgttaaa tatgacggat cactggcaaa taagacacct attctatgat atttaacgga
     4441 cttttcaaga gaagcgctta gctgttttgg caaaggcccc gcacagtact gataatgcgt
     4501 ttttgggacc tggcacaagg tttgcctata ttctcattgt ctgggggggg aagctgcaag
     4561 ccgtggaacg tacagagggg tcgtttttgg gagaattagc cacctgtcgg cggccacacc
     4621 ggtgagttgg gatagggaca gctgaccgcc tgccgggtgt gtgagatggg agacacgacg
     4681 catggcggac aatgcgctag tcggcaaatc aatccaagcg gcgggggtgc ctcggcgttt
     4741 ttggcttctc gggctcggca tggcaggcgt cttgggtttc cttgcggtac ataccttgta
     4801 ccccgaatgg catctgctgc cgcaggcgac aagccagtac cgtccggcga cgctgctggc
     4861 cgtgtttctg gcggcattca tttgcgagta tatcgattcg gccctcggca tgggttatgg
     4921 gacgacgctg agcccgatgc tgctgatgtt cggttttgac ccgcatgcag ttgtgccctg
     4981 cgttctgatc agcgaactga ttaccggcgt gacggccacg ctcatgcacc atcgggacgg
     5041 caatgtggac tttttgcggg acaagaatgc tcgcggaacg gccatcctgc tttctgttct
     5101 gagtgttgtg ggagcggtga tcgcggtccg actagccaac aagctttctg ttccagggct
     5161 gaggttggcg atcgccacgg tgatcctggc gacgggcgtt ttcacgctgg ccacggttcg
     5221 gcggcagctt cgttaccgca agagccatat tatcactgtg ggggccattg cggcgttcaa
     5281 caagggtctc agcggaggcg ggtacggccc gttggtaacg gcggggcagg tcgtctcggg
     5341 gctgtctcca aagcatgccg tggcgatcag ctctctggct gaaagcttcg tctgcctggt
     5401 ggcgatcatc gcctcgctgt ggctggggaa ggacctgaat tggggtctgg ctttgcctct
     5461 ggccgtcgga gcggtgatgt cggttccgtt ggccacgatg acggttcgct ggttgccgga
     5521 gaaggctatg cgggccagcg tcggtgtgat gaccattgtt ctggcgattg tcatgctgac
     5581 cggcgggaaa gcatgagtgg ggccgggggt tttgaggcac atcacggcgc actggcgtca
     5641 tagaacggcg ggcctatctg gaagtgtgag gaaacgacgg agcggtcagc ccgggtgagg
     5701 ccggtcgctg cgtttttccg ttttgtgagc cgtggggggt ggatcagtcc gttggtgacg
     5761 cgggcggtgc cgtgcgaaag ccggccactt gctcgacatc ggttcggcgg tcctgggcga
     5821 tcatccgaag cgcatcctcc aagcctgtgt cgatcaggat ggcggtttga tcgtcagcgg
     5881 agaccgcaaa atcgcaattc aggtgtatgg ccgtttgtgt gccgtagggc gtgttccggg
     5941 agcgggcctt tttccaatca ggcgtgtgac catctttgac ggcaatgctt tcctcggggc
     6001 agatgagccg cacctggctg taccgccctg gggccaggtc ggcgctggcg agtacgcagg
     6061 gccgtccttc gtttgtttgc agcagatcga tgacttgctc gccgtcgcgg atgacgaccc
     6121 aggcatcgtg attgtccgca tgtcggacct cgatgcgcga gaccgtcagc ctggttgtcc
     6181 caacccgctc cgcaaggcat ggactgtttt tgacaagcac ccgaagtgtg cccgtttccc
     6241 tgtggggccg aggggcgaag ccgcgtccca cagaagaacc gccaagaaga agaccgaggg
     6301 tgatcgccaa cacgcagacc gacgaacacg cgccgatacc gatacgcttt gcgaatgtgt
     6361 gcatcacgtt aaatatagta aatgtggcgg ggcaagggcg ggcaattcga tcagccgacc
     6421 gcatctgttt caatgggcgg gggttgtcaa tcggttgtta tcgggagtcc ttttgtgact
     6481 gcgcctgcaa acgtcatgtt ttcggactca ccgggtagcc ggcccgccgg aaaaccatga
     6541 gccccgagca ggatcgctgc ccggggccct gttggcattc tcaggttgtg ctttggggga
     6601 tcattccgcc ttatagctga tcccggtcag cggtttgccg tccgggtcgc ggatggcaca
     6661 gcagaaggcc cagtcaccgc cgccctgaac aaccttgata agcaatgtgt tttcgccctt
     6721 tttcagcgtc gccttcactt tgtcctggtc gcaagcgcag gggcggttga ccttgttggc
     6781 atggatcaac tgtccgttga gccagacctt gatcgcatcg tcgctgccga aggccatctg
     6841 cacctctcgc tcgctttcgg agatgaccgt cgtccgcacg taggcgcagc aattgttgcc
     6901 cttgccgaga tcgaagtgcc cgggatggtc ggggttggtg acattgagcg gcttccattc
     6961 cttaacattg ccctgcggtt cctcgggcgg aaagacgacg tcaaaggttt ccaggccttc
     7021 cttgcccttc tgcttgtagg ggccgctgac catccaggac gtgcagtagc tcgtcagcgc
     7081 gtcccgcagg gcgtcaatgg cgttcttttc cgagtcggtt tttgtgagtc tggagagctt
     7141 gtcgacagcg tcgagagccg ctgcatggtt gcgtccggca atgcgctttg acaccgacac
     7201 cgccgccgca aacgcttcag cgtgcagatc gccgtcgacc agcgacatca attcgtcgag
     7261 tgccttgacg ctggggatat cgccgattgc cgacacgatg agtttcttgt catcggcgtt
     7321 attggcgaga ctcattgccg tagtcagggt ctggaggcgc tcctcggggc cgccctgttc
     7381 gcgtgccagc cgcacgtaac cgcgaagggc gaccgagcgg tgcgttgcgt tctcgctcga
     7441 cttggcgatg agcagcaggg catccatcgc cgaggcgtcg ccgagctggg ccagcgccag
     7501 aaccgccgca tccttgaccg cgggttcctt ggcttcgcgt gccagccggg ccactgcctg
     7561 acgggcttcg gggccgcgct ggccgagaac gtcgatgatc agttccgccg tttcgggttg
     7621 tgcgtttccg agagcggcca gcagcttccg ggtcacctcg tcgccgggga tttcctggat
     7681 gctgcgagcg gcgaccatcc tcatgcgggc gtcatcgccg ttaaggacgt cgaacagcat
     7741 ggggagggcc ttggtcccgc gtgcggcggc aatgccctgg aagccggcga tgcgaatact
     7801 ctccggccgg ctcttgtcat agagccgctc gtaaattgcc gctgcctggt cggccgagcc
     7861 cttggcgatc aactcttcgc cgcatttcat ggccgcttcg gctatcgagg cgtgtaatgt
     7921 ggggcgaggc ttttccagca gtgcggtgac ggccttgatt gcctcgggcg tccgaatgtc
     7981 gcccagggcg cacacggcgg cggtcgccac ggcgtcatcc gggctgctcg tcaacttggc
     8041 gattgcgtcc accgcctgac cgtccctgcg aaatgcgagg ccgttgatca tggcgatctg
     8101 ccacttggac gtgtcggctc gggccaactc ggcccgaagc gatgctccgg cttgcggcga
     8161 gggattgttt acgagcgcgc gccgggcggt ttcgcggatg tcggcgtcct catcgcgcag
     8221 cagctctgtc agcctggcca cgacctcgtc gcggcccagg ggctcaacct tgcgcaggat
     8281 ccagatgcgg gccggcttgg gcacatcggg gcccaccttg gtcatcattg ctctgcagag
     8341 ggcttcgcgc tcggctgctt tgccgggagc cccggcctcg tggcagagct tttcgagggc
     8401 ctgttgggga tccttgcgtt ttgggatgtc cggatcgctc atgccgggta tccactcggt
     8461 aatcttgtca atgtactgct tctccagatc ggatgcaggc gcagccgccg tggtgttcaa
     8521 gagacaggcc gttgccgcca gagtcagcag cccaagaagc gtgcattttc tcatcatgtg
     8581 atgttctcct cagttgttac agacgccatg gagcccgttt ggggcggtcc atccagcggc
     8641 ttgcctcgct gtcaccgatg atttcccgtt tttccgggtc ccatttgatt gggcgcccca
     8701 gccagtaagc gatgttggac aggtggcaca cggtcaccga gctggccccg atggccacat
     8761 cgcaaatggg ccgcttgcgc gagcggatgc agttgatcca gtcctgccga tggcccgggc
     8821 tcttgtagag gtgaacatcg ttcgggccga gaggttcttc ggcgatcgat ttgggctcgg
     8881 cctggaagta acctcggttc acctcgatgt cgccttccgt cccgctgaag agcactccgt
     8941 tggcctgggc ccgctgcatg atcacgccgt tggcatactt gaaggtcatg tgttcgacgt
     9001 ctttgccgtt gggcggaatg atctcgacgg
//
LOCUS       DAOTPW010000234         9019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7024_length_9019_cov_5.586011, whole genome shotgun sequence.
ACCESSION   DAOTPW010000234 DAOTPW010000000
VERSION     DAOTPW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7024_length_9019_cov_5.586011"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="P5316_17585"
     CDS             complement(<1..426)
                     /locus_tag="P5316_17585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRR86823.1"
                     /translation="MSFLHPWMFSLGAVALGLPVLIHYLTRPRPVRLPLSTIRFVQEV
                     IHQQRTFHRLRNFLILALRTLAVGLLAWAFARPLIGEKPLIGKDDSVSAVRVVILDVS
                     QSMAASARGISVFERARSTAAEYLIDRAGLRANLILAGTK"
     gene            complement(688..1608)
                     /locus_tag="P5316_17590"
     CDS             complement(688..1608)
                     /locus_tag="P5316_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRR86824.1"
                     /translation="MSAPPTESRSRYLDLRALASLEHMRFTTRHRIEGVYSGRHQSRS
                     HGGAGEFVDFREYVEGEDLRRLDWKVLARTGRAYLRLYQDETNLLCTLAIDASGSMLF
                     GSRGRGGTQGSKLEYVQYLATALSHVISRQQDQVGLALLTNRLAEMIPPGSTRGHIAQ
                     IQERIERIVTEPVTRMADGLRELFERSPRRGVLMLMSDFLDQDLEAVFSSLRLFRHQY
                     WEVILLHVIHPDEERLPQGLAYRFDGLEKEGHIDCSPADIRVMYEQRFAAHTAGIRTS
                     ALAGGCDYRRVSTAIPYLQTLAGFLVERAG"
     gene            complement(1630..2637)
                     /locus_tag="P5316_17595"
     CDS             complement(1630..2637)
                     /locus_tag="P5316_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRR86825.1"
                     /translation="MISESTDVHLAREKVESFTERVQAIRESLHQVVVGQDVTIDLLL
                     TCVLTGSHALLVGVPGLAKTLMVKALASAFRWKFKRVQFTPDLMPSDITGYELLSRDE
                     QTGQPRMTFRPGPVFANLLLADEINRAAPKTQSALLEAMAEKHVTVGGETHELAEPFL
                     VVATQNPIEQEGTYPLPEAQLDRFMMEIHIDYPTPEQEEEIVEKTTGGTPPLPEAAFD
                     RAEFLELRDLVLSVPAPKSVVRYAVRLCGASRPADPRADSYAKDYIAWGAGPRGPQNL
                     MWAAKARALLEGRTAPIIEDIRALALPILRHRIIPNHRAVGDGVNSERIIHHLLETVA
                     H"
     gene            complement(3553..3732)
                     /locus_tag="P5316_17600"
     CDS             complement(3553..3732)
                     /locus_tag="P5316_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86826.1"
                     /translation="MSKLKEWLLIGMVALVGAASLGSDCNFTLRGDEDGIRFDVDDDD
                     DDLEDLFDDIKDLFD"
     gene            complement(4279..5313)
                     /locus_tag="P5316_17605"
     CDS             complement(4279..5313)
                     /locus_tag="P5316_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRR86827.1"
                     /translation="MFSMPRQCVTHQVICATGWTIVWISLAFVWTASAVVAEQPAATS
                     RPASQPSELVVALLQMAPAGSDREANLTKADRFCRDAAARGADIALMPEMWSVGYCGF
                     DQKQPGAREAFQAMAVGTGSPWVQHFSRLARELRMAIAVTYLQAWDGAPRNAVTLFDR
                     HGKEMFTYAKVHTCDFAPMEASTTPGTDFYVAEMDTRVGSVKVGAMICFDREQPESAR
                     ILMLKGAELILVPNACELEELRLDQFKIRAWENVLDVAMANYPKPNNNGHSVAYDSSG
                     KCRVIADEQEGLHLASFDLAQLRKARSESIWGGAYRRPHRYRQLLSLEQEDIWHRNDA
                     FGKPFDPSQR"
     gene            complement(5450..7270)
                     /locus_tag="P5316_17610"
     CDS             complement(5450..7270)
                     /locus_tag="P5316_17610"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRR86828.1"
                     /translation="MNADTSPDKLAGSVGIVRTQTVRLFEPPDVLHLECGRTLGPIDV
                     AYETYGRLNESRSNAVLICHALSGDAHVAGYHSPNDRKPGWWDIMIGPGKGIDTNKYF
                     VICSNVLGGCRGTTGPSSINPKTGKPWGMDFPVITIEDMVKVQKKLIDYLEIKKLLAV
                     IGGSMGGMQVLEWAVRYPDAVAGVVPAATTARLSAQAIAFDAVGRQAILADPHFADGQ
                     YHSGPGPDNGLAIARMVGHITYLSEQGMHAKFGRELRHRANYSYDLESNDFAVETYLD
                     HQGQAFVERFDANSYLYITKAIDYYDLQAGHESLKDALKDVKARFLVISYSSDWLFTP
                     RQSRQIVDALLANDKDVSYSEINSPYGHDAFLLETEVLGRLISGFLADLSGRPIIADR
                     GDVEPYRQRPVVETAWAHHRIDYDRIEEMIEPGSRVLDVGCGSGALLSRLMRHKNIRG
                     LGIEVEQDNICECVEGGIPVVDLDVETELSGFATKSYDYVVLSQTLQTLNRPELVLRE
                     ILRIGRRCIVSFPNFVQWRPILQMLLTGRTPVTQNLPFQWYNTPNRHYLTIRDFQAYC
                     RDNHIQVLRMIPLAEGRRDPVRFLPSVRAAEAIFVISKRD"
     gene            7357..7578
                     /locus_tag="P5316_17615"
     CDS             7357..7578
                     /locus_tag="P5316_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86829.1"
                     /translation="MMTMSLIDQYRLDRTKFSVGSLDEPSDEKAFWLNQTPAERLRAL
                     EYLRQVAYGYDPAATRLQRVLEVAQREEG"
     gene            7556..7960
                     /locus_tag="P5316_17620"
     CDS             7556..7960
                     /locus_tag="P5316_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013320616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86830.1"
                     /translation="MLNEKKVEYLVVGGYAVAFHGYPRPTGDLDIWIAVHPKNIDGVV
                     EVLREFGFGGDDTSKDVFTKPGVIIRMGVPPVRIELQTRISGVSFEECYQHRIVGDFD
                     GEPVNLIDLDHLKQNKRAAGRNKDLADLENLP"
     gene            complement(8462..>9019)
                     /locus_tag="P5316_17625"
     CDS             complement(8462..>9019)
                     /locus_tag="P5316_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRR86831.1"
                     /translation="HGVVFAEALKPIGNIAYIIKCRTHFLRDMGSCMSPFAAFLFIQG
                     LETLHLRMARHCENAMKVAQHLKGHPAVQWVNYPGLPDHRDHAHAKKYLPKGCGAILG
                     FGIKGGLEAGKKFINSVKLLSHLANIGDAKSLCIHPASTTHSQLTEQEQAQTGVTPEY
                     VRLSIGIEDVEDIIADIDQALAASQ"
BASE COUNT         1695 a         2806 c         2736 g         1782 t
ORIGIN      
        1 tttcgtcccg gccaggatca ggttggcgcg cagccctgct cggtcgatca ggtactcagc
       61 agccgtcgac cgggcccgct cgaacacact gataccccgc gccgaagcgg ccatgctctg
      121 gctgacgtcc agaatgacga cccgtaccgc gctgaccgaa tcgtccttgc cgatcagcgg
      181 cttctcgccg atcaacggcc gggcgaacgc ccacgccagc aggcccacgg ccagggttcg
      241 cagcgccagg atcaggaagt tgcgcagccg gtggaatgtc cgttgctggt ggatcacctc
      301 ctgcacaaat cgtatcgtcg aaagcggcaa tcgaaccggt cgcggacgcg tgagatagtg
      361 gatcaagacc ggcaggccaa gggccaccgc tcccagagaa aacatccatg gatgcaggaa
      421 gctcattgcc cggcggcctc cctgggcgtg cgatgagggt tcagggttcg gggttcagag
      481 gagaaaggat gaagtatgaa ggatgaggga tgaagggtga gggatgaggg atgaaggaca
      541 gacgaaccag agccaatcgg ggcctgtggc ggtcggttca tgcggatctg gcgctcacac
      601 aatcggctgc gtggcgttac cttagactcc ggcgtgtctc tgattggatt ccttgttttc
      661 atccttcagc cttccgtctt catccttcta ccccgctctc tccaccagga accccgccaa
      721 cgtctgcaga tacggaatcg ccgtcgacac ccgccgatag tcgcagcccc cggccagcgc
      781 agaggttcga ataccggccg tgtgggccgc gaatcgctgc tcatacatga cccgaatatc
      841 cgccggcgag cagtcgatat gcccttcctt ctccagaccg tcgaagcggt atgccagccc
      901 ctgcggcagc cgctcctcgt ccgggtggat cacgtgaagg agaatcactt cccagtactg
      961 atgccggaac agcctcaacg atgagaagac cgcctccagg tcctgatcga ggaaatcgct
     1021 catcagcatc agcaccccgc gtcgaggcga gcgttcgaag agctctcgca gtccgtcggc
     1081 catccgggtt accggctcgg tgacgatcct ctcgattcgc tcttggatct gcgcaatgtg
     1141 tccccgagtg ctgcccggcg ggatcatctc tgccagcctg ttcgtcaaga gggccagccc
     1201 gacctggtcc tgctgcctgc tgatgacgtg actgagcgcc gtggccaggt actgaacgta
     1261 ctccagtttc gatccttgtg ttccgccgcg gccacgtgat ccgaacagca tcgatccgct
     1321 ggcatcgatg gccagcgtgc ataacaggtt cgtctcgtct tggtacagcc gcaggtaggc
     1381 gcgcccggtc cgagccagaa ccttccagtc caggcggcga aggtcctcgc cttcgacgta
     1441 ttcgcggaaa tcgacgaact cgcccgctcc gccatgcgac cgagactggt gccgcccgct
     1501 gtagacaccc tcgatccggt gccgtgtggt gaaccgcatg tgctcaagcg aggccagggc
     1561 tcgcaggtcg agatatcggc ttcgtgactc ggttggcggc gcgctcatgg cgggctcccg
     1621 tccagaccgt cagtgagcaa cggtttccag caggtggtga atgattcttt ccgagttgac
     1681 gccgtcgccg accgcccgat ggttcggaat aattcggtgt cgcaggatcg gcagggccag
     1741 tgctcggatg tcctcgatga tcggtgccgt acgcccttcc agcagcgcac gggccttggc
     1801 cgcccacatc agattctgag ggcctcgcgg accggcgccc caggcgatgt aatccttcgc
     1861 gtatgaatcc gcccgagggt ctgcaggccg gctcgcgccg cacagccgaa ccgcatagcg
     1921 gacgacgctc ttgggagcag gtactgacag caccagatcg cgaagctcca gaaactcagc
     1981 tcggtcaaac gccgcctccg gcagaggcgg cgttccgccg gtggttttct caacgatttc
     2041 ctcctcctgc tcgggagtcg gataatcgat gtggatctcc atcatgaagc gatcgagttg
     2101 ggcttccggt agcgggtagg tgccttcctg ctcgatgggg ttctgcgtgg ccaccaccag
     2161 gaacggctcg gccagctcgt gcgtttctcc cccgacggta acgtgtttct ccgccatcgc
     2221 ttccagcaaa gccgattggg ttttcggggc ggcccggtta atctcgtcgg ccagcagcag
     2281 attggcgaag accggccctg ggcggaaggt catccttggc tggccggttt gctcgtcgcg
     2341 gctgagcagc tcatagcccg tgatgtccga gggcatcagg tcgggcgtga actgcaccct
     2401 cttgaacttc caccggaagg ccgacgctaa ggccttgacc atcaaggtct tagcaagtcc
     2461 cggtacgccg accagcagag cgtgcgagcc ggtcaggacg caggttaaca gcaggtcgat
     2521 ggtcacgtcc tgacctacga ccacctggtg caggctctct cggatcgcct gaacccgctc
     2581 agtgaacgat tcgaccttct cccgcgccag atgcacatcg gtgctttctg aaatcattgt
     2641 ccgtcccctg ctgaatggat tctgttcgag cgcggggccg aacccggtcc ccgccccgtt
     2701 ttgtgaggtc aaacccgagc gccgcttctg cccgtccccc gtcaaagtgg gtcgaggaaa
     2761 ccgctagtcg ccgggggcag ttctccgcct catgccggac aaaaagcata cagcgccgga
     2821 ggcaactgct tccagtcgag gagccactcc cggccaagtc gcttcccgcc gcgccaaggc
     2881 cccatgaata gagaccccct tacaatgcaa ttcttacaat gccgtcccga accggcgttg
     2941 ttgtttcaag gcagaggctt ccagatgctc tcagggacgt taaaccaggt tgtcttgctg
     3001 ctactacaca tatatacgcg caaacacccc aaaagttccg gggaagacca gtcaaaaagc
     3061 acgaaatctg gggaagcggt cctgttttcg cgaccccgct ctgcccaacg ggctggtgtc
     3121 cattcgcact gcaacccgca gccacggttc tctgcggacg aagccggtcg ttgtttctgg
     3181 aatggcgact ggtggtcgag gcagcaccca tcgccgctga gtccccacct tcggcccgtc
     3241 aacgggtctt ccggacgcca gccgtgccca tgtgaggcct caggtgttgc agatccgggc
     3301 cggttttgaa catgatcagc tttggcagcc tggccatgag cttgcttggg gcgtccttgc
     3361 tcaggggggt tggggccgtg aaggtcaagt ttgccggatt cgctggttcc cgaattccca
     3421 tccgggaccc tgcagttgac gattcccatc gtcaatcatc tgggaatgtg gaaaggattc
     3481 catgaaaacc acggcccaga agtgattctg gaccgagcga tctgacggtc ttggccgcga
     3541 aggctggagc ggtcaatcga agaggtcctt gatgtcgtcg aagaggtctt caagatcatc
     3601 atcatcgtcg tcgacatcga accggatccc atcctcgtct ccgcggaggg tgaagttgca
     3661 gtcggatccg agcgaggcgg ccccgaccag ggcgaccatc ccgatgagta accattcctt
     3721 caacttggac atatcgttct cccttctggc ttctcaaccg ccgacggccg tgggtgcagg
     3781 caccttccgg gaacgcagta tcggcaatgc ccgatcggat cacccctggg ccggcccctc
     3841 cttgccaccg actcctgccc ttgcgggctt ccgcgccgct attgccttta ttatcgtagc
     3901 caagcgtgcc gaaaattgag ccaaacccct gggtccggcg gtcccccgta tcgagtgaac
     3961 actggcgggc aagccgccgg tggcaccggg cgtacaggtt gggccgacgc cctcgtcggc
     4021 ccttggttga acgagggatg aagtgccgac gggggcgctc gtgctgcgca gcgggtcgcc
     4081 gcttcgcaga gcaacgtcgg caccacttgc cgggtgcaaa gacttggtag ttcacggcgc
     4141 aatccgggtc ggtcgaaatg ctcgaccccg acagaaaacc ggcgacgacg gcgaagtcgg
     4201 cttggtcgac gtcgatgtcc ttgatgcagt tgtcgatgat caaccaggga ttggaacact
     4261 cggctccagg aaatctgcct atcgctgtga tggatcaaac ggcttgccga aggcgtcatt
     4321 gcggtgccag atgtcctcct gttccaggga cagaagttgc cggtaccggt gcgggcgacg
     4381 gtaggcgccg ccccagatcg attcagagcg tgctttgcgc agttgggcaa ggtcgaacga
     4441 ggccaggtgg agcccttctt gttcatcggc aatgacccgg cacttgcccg acgagtcata
     4501 ggcgacggag tgaccgttgt tgttgggttt cgggtagttc gccatggcca cgtccagcac
     4561 gttctcccat gcgcgtatct tgaactgatc cagcctgagt tcctcaagct cgcatgcgtt
     4621 gggcaccagg atcagctcgg cccccttcaa cataaggatg cgggcactct ccggttgctc
     4681 gcggtcgaag cagatcattg ctcccacttt gaccgaaccg acccgcgtgt ccatctcggc
     4741 cacatagaag tccgttcccg gcgtcgtcga agcttccatg ggggcaaaat cacaggtgtg
     4801 aaccttggca taggtgaaca tctccttgcc gtggcgatcg aacagggtga ccgcgttccg
     4861 cggggcaccg tcccatgcct gcaggtacgt gaccgcgatg gccatgcgaa gttctctggc
     4921 caaccttgag aaatgctgta cccaagggct gcccgtgccc acggccatgg cctggaacgc
     4981 ttcgcgggca ccgggctgct tctggtcgaa cccgcagtat ccgacgctcc acatctccgg
     5041 catcagtgcg atgtcagcac ctcgggctgc cgcgtcacgg caaaagcggt ccgccttggt
     5101 gagattggct tcccgatcgc tgcctgccgg ggccatctgc agaagagcca cgacgagttc
     5161 agaaggctgc gaggcgggtc ggctcgttgc cgccggctgc tcggcgacca cggcggaagc
     5221 tgtccacacg aacgcgaggg agatccaaac gatggtccat ccggtggcac agatcacctg
     5281 atgtgtcacg cactgtcttg gcatgctgaa catactcacg ggtgcttcct ccagactgag
     5341 gccaatcatg ataaccgcag cccgcgaggt ttgcctgtgg cggagtacac ccatgggtgc
     5401 ggggtcgcgc ctctggacaa gctgcggggg catcagatcg agccgaggct cagtcgcgct
     5461 tgctgatcac gaaaatcgcc tcggccgccc ggacgctggg gaggaagcgg accgggtcgc
     5521 gacggccctc ggccagcggg atcatgcgca gcacctggat gtggttgtcg cggcagtagg
     5581 cctgaaaatc gcgaatggtc aggtagtgcc ggttcggggt attgtaccac tggaaaggca
     5641 ggttctgcgt gacgggcgtg cggccggtca gaagcatctg aaggatcggc cgccactgaa
     5701 cgaaattggg gaaactcacg atgcatcgcc gcccgatccg gaggatttcc cgcagcacca
     5761 gttccggccg gttcaacgtt tgcaaggtct gcgacaatac cacgtaatcg taggacttgg
     5821 tcgcaaaccc ggagagctcc gtctcaacgt cgaggtccac gaccgggatg cccccctcga
     5881 cgcattcaca gatgttgtcc tgctcaacct cgatgcccag cccgcggatg ttcttgtgcc
     5941 gcatcaggcg actgagcaga gcgcctgatc cgcagcctac gtccaacacc cggctgcccg
     6001 gttcgatcat ctcttcaatg cgatcgtagt cgattcggtg gtgcgcccag gccgtctcga
     6061 ccaccggccg ctggcgatac ggctcgacgt cgcctctgtc agcaatgatg ggccgtcctg
     6121 acaaatccgc caggaaaccg ctgatcaatc ggcccagaac ttccgtctcc agcaggaacg
     6181 catcgtgccc gtagggcgag ttgatctccg agtacgacac atccttgtcg ttggccagca
     6241 gggcgtcgac gatctgccgc gattgccgag gggtgaataa ccagtcgctc gagtagctga
     6301 tgaccagaaa ccgggccttc acgtccttga gagcgtcttt cagcgactcg tggcccgcct
     6361 gaaggtcgta gtagtcgatc gccttggtga tgtacaggta gctgttggcg tcgaatcgct
     6421 cgacgaatgc ctgtccttgg tggtcgagat acgtttcgac ggcgaaatcg ttgctttcca
     6481 ggtcgtagct gtagttggca cgatgacgca actcgcggcc gaacttggcg tgcatgccct
     6541 gctcggacaa ataggtgatg tgcccgacca tgcgggcgat cgccaggccg ttgtccggac
     6601 cggggccgct atggtactgg ccgtcggcaa agtgggggtc agcgagaatc gcttgccggc
     6661 caacggcgtc aaacgcgatc gcctgggcgc tgagccgggc ggtggtcgcc gccggaacaa
     6721 cgccggcgac ggcgtccggg taccgcacgg cccattccag cacctgcatc ccgcccatcg
     6781 accccccgat gaccgccagc agcttcttga tttccaggta atcgatcagc ttcttctgga
     6841 ctttgaccat gtcctcgatg gtgatcaccg gaaagtccat accccagggt ttgccggtct
     6901 tcggattgat gctcgacggg ccggtcgtcc cccggcaccc gcccaaaacg ttcgagcaga
     6961 tcacaaaata cttgttcgtg tcgatgccct tgcccggacc gatcatgatg tcccaccagc
     7021 cgggcttgcg gtcgttggga ctatggtacc cggccacgtg agcatcgccc gagagagcgt
     7081 gacagatcag aaccgcgttg ctccgcgact cgttgagccg gccgtaggtc tcgtaggcca
     7141 cgtcgatcgg ccccagggtc cgcccgcact ccaagtgcag gacgtcaggg ggctcaaaga
     7201 gcctcaccgt ctgcgtccgg acaatgccga cggagccggc aagcttgtcg ggcgaggtgt
     7261 cagcgttcat gctatcgaag cattatagac acggtctccg gctggcgcaa ccaggccgtg
     7321 cgtggttgac tcgtcgggcc caccgggata agctgaatga tgacaatgag cctgatagac
     7381 cagtatcgtc tggatcgaac caagttctcc gtcgggtcac ttgatgagcc cagcgacgaa
     7441 aaggcgttct ggctaaacca gacgccggct gagcgcctcc gggcccttga gtaccttcgc
     7501 caggttgcct atggctacga ccccgctgcc acgcgacttc aaagagttct tgaggttgct
     7561 caacgagaag aaggttgagt accttgtggt cggtggctac gccgtcgctt tccacggtta
     7621 tccgagacca acgggcgatc ttgatatctg gatcgcggtt cacccgaaaa acatcgacgg
     7681 agttgtggag gttctccgcg agtttggctt cggaggagat gatacgtcca aagacgtatt
     7741 tacgaagccg ggcgtgatta tacggatggg cgtgccgccg gttcgaatcg aactccagac
     7801 ccgcatctcg ggggtctcct tcgaggaatg ctatcagcat cggattgtcg gtgacttcga
     7861 cggcgagcca gtcaacctca tcgatcttga tcatctgaaa caaaacaaac gcgccgccgg
     7921 tcgcaacaag gatcttgccg acttggagaa cctgccgtag ttcccggctt gctctcgggt
     7981 cggagtccat gagcatcgcc gacgaccgag aataccagat aacgcagaac aaactggccg
     8041 gggtacgtga ggcgatcgcc cggatcgaga aagctcccct gaccggctct ccggtcgccc
     8101 gctctgcctc gttacggtcg ctccgtgtct atgaaaacca gcttgtcgag gaacacgctc
     8161 ggcccggaat cctttccggg cctcttctct ccgcggaatc ctttccgcat tcggtgaaaa
     8221 tgaggatggg aatcctcaac gcccaaggcc ccagaatgga ttctggggcc agcagcaatg
     8281 gattctgggg ccagcagcaa tggattctgg ggccagcgag aatcaccgct gcttccggag
     8341 ttcaccggtg gtgaggttca tgggcaacgg caaaacacgg ctcactctca attctacatt
     8401 cacaattctg cattccccgg cgttgccgta tgcaacgctg gggtaaagcc accggctcgt
     8461 tttactgtga tgccgccagg gcctgatcga tgtccgcgat gatgtcctcg acgtcctcga
     8521 tgccgatcga cagtcgcacg tattccggcg tgacgccggt ctgtgcctgc tcctgctcgg
     8581 tcagttgcga gtgcgtcgtg ctggccgggt ggatgcacag cgacttggca tcgccgatgt
     8641 tggccaggtg ggacagcagc ttgaccgagt tgatgaactt cttgccggcc tccagcccgc
     8701 ccttgatgcc gaagccgagg atcgcgccgc accctttcgg cagatacttc ttcgcgtgcg
     8761 cgtggtctcg atgatccggc aggcccggat agttgaccca ctgcaccgcc gggtggccct
     8821 tgagatgctg ggcgaccttc atggcattct cgcagtgcct cgccatccgc aggtgcagcg
     8881 tctccagtcc ctggataaag agaaacgccg cgaacgggct catgcaactg cccatgtctc
     8941 gcaggaagtg cgttcggcac ttgatgatgt acgcaatgtt gccaatcggc ttgagcgctt
     9001 cggcaaaaac cacgccatg
//
LOCUS       DAOTPW010000235         9005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7037_length_9005_cov_4.819665, whole genome shotgun sequence.
ACCESSION   DAOTPW010000235 DAOTPW010000000
VERSION     DAOTPW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7037_length_9005_cov_4.819665"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="P5316_17630"
     CDS             complement(<1..1288)
                     /locus_tag="P5316_17630"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRR86832.1"
                     /translation="MRYTLALLMLANSLSPRMGTVCAQSIFNPDVQGTGATMGDYMIA
                     VTGGGQPGGPQYTYRMGRFEITNQQFCDFLNDAERDAQRAPRTRRSTNMFFGPHGSVY
                     LDGNMSANEPIFLSHNDVFTSDIRYDPLMPVGRRYSVISDGFGSKAKHPVRHISWMGA
                     MKFCNWLTIDQGLGQEHCCYTEGPHIGNWHPITINTIDWWGKEPLHDDPYTAGRNPND
                     AERTELVRRYRGYRLFMDDTGLLAPISRPDPKDFNEWLKAAAWDPNAPGTFRTNSGGW
                     QAAPGHWMYGFGREVNTGADANFYDSGDPWDNGPVPVDFYDGTDHGGTFATNATANRY
                     GFYGMAGNVWEFSQDYGPGPRHCAIYGGSWVSSAEKQAASSVYVNALITWADITFGMR
                     LVQVGSPLEVKVFAGTPAAHDEFGRTVAICGDCAVVG"
     gene            2110..2766
                     /locus_tag="P5316_17635"
     CDS             2110..2766
                     /locus_tag="P5316_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86833.1"
                     /translation="MRKVLMGLVVVSAITASASAANLLTNGSFEDGMNGWTKATFYWA
                     GWGTGAPAINSITGLGTFGGFGGPQDGSRYAGFDISGHANYHVGAYQTFATEVGQTYD
                     VTGWFAGGREAANDSMWWEIKVSPGMTSDPDAAGTLIAKREVGPNDPWFSFTEDFAGQ
                     FVATDSMTTVFLKVGRVQSADWKISAAVFDNLKVELVPEPAAALLLLAGLPLLRRRRA
                     "
     gene            3115..4119
                     /gene="fliM"
                     /locus_tag="P5316_17640"
     CDS             3115..4119
                     /gene="fliM"
                     /locus_tag="P5316_17640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRR86834.1"
                     /translation="MADVLDQAEVDALLSAVSTGAPESGESQVFSRARAKPQEVRTYD
                     FKRPERVSKDQMRSLEAIHEGFARNFGASLSAFLRTIVEARVATIEQLTYSEFIHSLP
                     NPTCFNLLNCSPLEGQICLEISPLIIYPIIDRLLGGSNAELFIPQRALTLIEWRLVER
                     ITNRAVSTLTEVWSGLVDVKFELAETESNPHLVQIVAPNEVVVVIGLELKMGNRAGTM
                     TLCLPFNVIEPVMTKLATQSWLAYQRKAPSQEQQQRLATNLKRAEVEVRAFLAETIIT
                     MDDLLHLQPGDIIRTAKPANAELTLQVRDRSKFAGRIYQNKGMRVFRITRPIEGEEPL
                     "
     gene            4342..5367
                     /locus_tag="P5316_17645"
     CDS             4342..5367
                     /locus_tag="P5316_17645"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR86835.1"
                     /translation="MTGQDRVISVLKGGRVDRPPLLPILHSGLAGLLGVRLGDYFTDA
                     ATMARVAVEGCRRFGFDGVQLSLGVTGEAESLGAKVDQPADGAPVLRQHLLADVSNLS
                     SLKRGDAACRGRMPLFLDALRRVRSQAGSSLFCLATLRGPLNITAQLRGVEDTLVDMI
                     ESPDQISRILDFAVDVAITASEAALTASADGVVFGEATCSPNFISPELYRRLILPRHK
                     RLVRSLRSMGWKVVGFHVCGNILPILEDLISTGADLIDVDYQVPADQAVERAAGRVTL
                     RGNLDPSSVFRFGDSVKVRAQTRELCRVVSGARWILSSGCDIPPGTPAENLQAFAEAA
                     RDTCTGP"
     gene            5719..6924
                     /locus_tag="P5316_17650"
     CDS             5719..6924
                     /locus_tag="P5316_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRR86836.1"
                     /translation="MSGFVQSVLAVRMPQINVNDEEVTLIGWHVVDGQEVGADQPLCE
                     VETSKAVGDVPSPASGVVRLAAEVGDVIPIGQVFAYVGPSIEAIDAFIAARGDASTPS
                     VARRAAESSRVGGVDATVGAIELARRYGLDLAAVSASGRIRRSDVERFIAEKGLAAPV
                     GRAATTRDVVNLPPSLKDKVVDQGALPDHLRSIGEHLAQTQSHLVVAHVVMDACARGV
                     ADWIDSRRKAGVMTGPIPILLYAAAAAIAVEPRLRQFRLGGRAYSYRDIDIAFAARSG
                     DGRLFTPVVRGVDRLGLDDLARECARLCMAVFRGNLAVAEMSGACMTVSVLSEHPVRF
                     HVGLQNVWQSAILTAGAIHDEVRMDDGRPICVPVMTLTLSYDHGLMDGWEAAGALNEA
                     KSCIEAIGP"
     gene            6967..8103
                     /locus_tag="P5316_17655"
     CDS             6967..8103
                     /locus_tag="P5316_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86837.1"
                     /translation="MADDVSRSSLKPRPVRAEDAVSLHRLLVAELPDIPADFPRWLAR
                     WNWQYRDNPFRRDRPAGWVLADGEQVVGHLGAVYVPVGARGRFVIGAIGADYVVSQDA
                     LKAGGTFVGLQLAQAFFDGTSGCLRMATTANEKTGAVFGRFGCRPAEWTREFFRAPTG
                     LVQQIRTCRGGANRIVRRLMMGPLGLVGRSVLAAAYGLCGHRPALPIAPGCRLDIGMA
                     PLILEAASLHGRTAPVGFGIARSREYLDWRYVRHPESANIRALLVRRPEGEVIGAAVV
                     FLDRQDGRRIAYLEELTVATGDEAAMHTLVCAALRLAGDHKSHYLVCTAGRSDLRGRF
                     WEYGFESRARSAPALVIGGLEQQAGSASDDSLIAAAVFHHGDMF"
     gene            complement(8104..8712)
                     /locus_tag="P5316_17660"
     CDS             complement(8104..8712)
                     /locus_tag="P5316_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR86838.1"
                     /translation="MPTHPVQRFYRYQAGVYDLTRWMFLYGRQRAIDLLEIQRDSRVL
                     EIGCGTGLNFEHIVKRLDPARGRLVGVDFSQHMLGRAARRVASRGYVNLTVVGADAER
                     LALNERFDAVLFAYSLSMIPDWQSALERACGHLSPGGRMVVLDFGRFDRWGPLRRLAR
                     GWLWLNHVETGRPYETKLAELLTNLRVEAHLGGYYFVAAGRK"
BASE COUNT         1534 a         2598 c         3027 g         1846 t
ORIGIN      
        1 cgccgacgac ggcgcagtcg ccgcaaatcg cgaccgtacg cccgaattcg tcatgggcgg
       61 ccggggtacc ggcgaagacc ttgacctcaa gggggctgcc cacttgcacg agtctcatgc
      121 cgaaggtgat gtcggcccag gtgatcagtg cattgacata gacggacgaa gcggcctgtt
      181 tttcggcact gctcacccac gacccgccgt agatggcaca gtgccggggg cccggaccgt
      241 aatcctggct gaattcccac acgttgccgg ccatgccgta gaagccatag cggttggcgg
      301 tggcatttgt cgcgaacgtg ccgccatgat ccgtgccgtc atagaaatca acgggaacgg
      361 ggccgttgtc ccaggggtcg ccgctgtcat aaaagttcgc gtcagcgccg gtgttgacct
      421 cgcggccgaa gccgtacatc cagtgtccgg gcgcagcctg ccacccgccg gagttggttc
      481 ggaaggtacc gggggcgttg ggatcccagg cggcggcctt gagccattcg ttgaaatcct
      541 tgggatcggg ccggctgatc ggggccagca ggcccgtatc atccatgaac aagcggtagc
      601 cccgataacg gcggaccagt tccgttcgct ccgcgtcgtt ggggtttcgc cctgcggtat
      661 aagggtcgtc gtgcaggggc tccttgcccc accagtcgat tgtgttgatt gtgatggggt
      721 gccagtttcc gatatgcgga ccttcggtgt agcagcagtg ctcttggccg aggccctggt
      781 cgattgtcag ccagttgcag aacttcatgg cccccatcca ggatatgtgc cggacaggat
      841 gcttggcctt gctgccgaat ccgtcggaga tcacgctgta gcgccggccg acgggcatca
      901 acgggtcgta acggatgtcg gatgtgaaga cgtcgttgtg cgacaagaaa atcggttcat
      961 tggccgacat gttgccgtcg agatagacgc tcccgtgggg accaaagaac atgttcgtgg
     1021 atcggcgggt gcgcggggct cgctgagcgt cacgttcggc gtcgttgagg aagtcacaaa
     1081 actgctgatt ggtgatctcg aacctgccca tccggtaggt gtactgaggg ccgccgggct
     1141 gccccccgcc ggtgaccgcg atcatgtagt cgcccatcgt ggcgccggtt ccctggacgt
     1201 caggattgaa gatgctttgg gcgcagacag tccccatacg gggagagagg ctgtttgcca
     1261 gcatcaataa tgcgagtgtg tagcgcatcc ttccccttct cgcagcttgg ctcttgatta
     1321 tacccggcgg atcgactcgt ggggcatcac ccccgctggc ccgatcgaac gagggcacgc
     1381 gatgcatcca acgagggact ctgagcgcgt cgcggacggc atgcccgggc ctgcaggtcc
     1441 gcggaaaaga ggggtgcttc caggcctggg ggatcgggcc gacacccggg ccgcgggtcc
     1501 gcggaaaaga gggtgcctgg gagcaggctg atggtggtac ccattcggac tccatatcac
     1561 catgggcaga cggcgcgcta ttcgagagat cgttcggggc ggttcgggca ggttcttggg
     1621 cccggttctc cgccggacgt ggcggtgccg tcttcgtaaa ccttttctga taagtaactt
     1681 gtggttcttg ggcaggtgag gctcgggtgg gtgcgctgac agatccaacc gaatcattgc
     1741 cgtccgtctc agtgctacag cctgaattgg cgattctggc ttgcggatcg tcggctggca
     1801 gggtatgcta ctgtttgggt ccggagcgaa tctcagacat cagccgagcc aatggcagcc
     1861 ggtttggtga ttctggtctt gtccggatcc ggatgagagg aagagcggag gaacatgggt
     1921 cgggaaaagc tggggggtgg actgaacgcc agagtcgggc gctcccaccg ggtccaattc
     1981 tttcgcacca agaatcttga gtatctcagg cacggtaagc gaggggatcg cttcagaggc
     2041 agcgtgtgcc actgggggga cagatcctgc cttgtgttgc cgggcagtaa ttagaggagg
     2101 aaacgagtga tgaggaaggt actgatgggt ctggtggtcg tgtcagcgat taccgcgtcg
     2161 gcgagtgccg cgaatctgct gaccaacggc agcttcgagg atggcatgaa tggttggacg
     2221 aaggccactt tctactgggc cgggtggggc actggggctc ccgcaatcaa ctcgattacg
     2281 gggctgggga cgttcggggg ctttggtggt ccgcaggacg gttctcgcta tgccgggttc
     2341 gatatcagcg ggcatgcgaa ctaccacgtg ggtgcctacc agaccttcgc caccgaggtg
     2401 ggccagacct atgacgtgac cggttggttc gccggcggtc gagaggcagc caatgacagc
     2461 atgtggtggg agatcaaggt atctccaggt atgacgtctg atccggacgc cgcgggcact
     2521 ctcattgcca agcgcgaggt cggccccaac gatccatggt tctctttcac cgaagacttc
     2581 gccggccagt ttgtcgcaac ggacagcatg acgaccgtct ttctgaaggt gggccgcgtc
     2641 caaagcgctg actggaagat atctgcggct gtttttgaca acctgaaggt ggaactggtt
     2701 cccgagcctg ctgcggcctt gttgctgctg gccggtctgc cgttgctgcg tcgtcgtcgt
     2761 gcataactga cgtcggccga tctgatcggc agtttgtatg accatgaaag accgtcgtgt
     2821 tgcgaaacac ggcggtcttt tcttttcctt gttccgcgct gtggccgggc gcggtttacc
     2881 cttggagacg gcgaggcagc ctgacggagt gtcacggacg gctgggcggg cgccaggagg
     2941 cctcgtgcga gagtgtgacg cctggaacgt cgcgtcgccg aggtgttacc ctgcctgtgt
     3001 atgagagcgg tcttgttatc ggacgtcgag tttctcaagt cacctccctt ggctgtccga
     3061 taaaccggca tgaggtgcgc atgaagagcg cgccgagggg agctatcgcc tcacatggcg
     3121 gatgtactgg atcaagcaga agttgatgca ctgctttcgg cggtctcgac gggcgcgccc
     3181 gagagtggcg agtcgcaggt gttcagccgt gcgcgggcca agccgcaaga ggtccgcacc
     3241 tacgacttca aacgccccga gcgggtcagc aaggaccaga tgcgctcgct ggaggcgatc
     3301 cacgaagggt ttgcccgcaa cttcggggcg tccctgtcgg ctttcctgcg gacgattgtc
     3361 gaagcccgcg tcgcaaccat cgaacagctc acttacagcg agttcattca ttcgctgccc
     3421 aaccccacct gcttcaacct gctgaactgt tcgccgctgg aagggcaaat ctgcctcgaa
     3481 atcagtcccc tcatcatcta cccgatcatc gaccgtcttc ttggcggatc aaacgccgag
     3541 ctgttcattc cgcagcgggc cttgacgctg atcgagtgga ggcttgtgga gcggatcacc
     3601 aatcgtgccg tgtccacgct gaccgaggta tggtccggcc tggtggacgt gaagtttgag
     3661 ctggctgaga cggaatcgaa cccgcacctt gttcagatcg tggctccgaa cgaggtggtt
     3721 gtggttatcg ggctggagct gaaaatgggc aacagggccg gcacgatgac tttgtgcctg
     3781 ccgttcaacg tcattgagcc ggtgatgacc aagctggcca cccagagctg gttggcttat
     3841 caacgcaagg cacccagcca ggagcagcaa cagcggctcg cgaccaacct caagcgagcc
     3901 gaagtcgagg ttcgtgcttt cctggctgag acgattatca ccatggacga cttgcttcac
     3961 ctgcagccgg gtgacatcat ccgaacggcc aaaccggcca atgccgagct gactctccaa
     4021 gtgagggatc gcagcaagtt tgccggacga atctaccaga acaaggggat gcgcgtcttt
     4081 cgtatcacgc ggccaatcga aggcgaagag ccgctctgag tcccgtcgca gattcctgaa
     4141 gagtgggcaa ccggccttga tggtggccgg cgttttgggc aacaggcaac gtccagcctt
     4201 cgagtcagcg gtgttccctg gggtcatgcg atcttgccgg agaccccggt tgcgcattgg
     4261 tcatttcgca agcctcttga aatgtggtaa gtcggccggc aatcagttat cgtgcccgtc
     4321 ttccggtgag ggctcggcct tatgacggga caagatcggg tgatcagcgt gctgaagggt
     4381 gggcgtgtgg accggccccc gctgttgccg atcctgcact ccggacttgc cggcttgctt
     4441 ggtgttcggc tgggtgacta cttcaccgac gccgcgacca tggctcgcgt ggccgtcgaa
     4501 ggatgccgcc gtttcggctt tgacggcgtt cagctttcgt tgggagtgac cggcgaggcc
     4561 gaatcgttgg gcgccaaggt ggatcagcct gcagacggcg cacccgtgct tcggcagcat
     4621 ctcctggcgg acgtctcgaa tctgagcagc ctcaagcggg gcgatgctgc atgccgagga
     4681 cggatgcctt tgtttctcga cgcactcagg cgcgtcagga gccaggccgg ttcatcgctg
     4741 ttctgcctgg ccacgctcag aggtccgctc aacatcactg ctcagcttcg cggagtcgag
     4801 gatactctcg tcgacatgat cgagagcccc gaccagatct cgcggattct cgatttcgcc
     4861 gtggatgtcg caatcacagc ctcggaggcc gccctcacgg cgtcggccga cggcgtggtt
     4921 ttcggcgagg ccacttgctc gcccaatttc atttcgcccg agctctacag gcgtctgatt
     4981 ctgccgcgac acaagcgact tgtgcgttcc ctgcggtcca tgggctggaa ggttgtcggg
     5041 tttcacgtgt gcggcaacat cctgccgatt ctcgaagacc ttatctcaac gggcgcggac
     5101 ctgatcgatg ttgattacca ggttccggcc gaccaagccg ttgagcgggc ggctggtcgt
     5161 gtcacattgc gaggcaatct ggatccgagt tcggtattcc gattcggtga ctcggttaag
     5221 gtcagagcgc agacgcgcga gttatgccgg gtcgtgtcgg gcgcccggtg gattctcagc
     5281 agcggttgcg acatcccgcc cggcacgccc gccgagaatc tgcaggcctt tgccgaggcc
     5341 gctcgcgaca cctgcacggg tccgtaacag gttcggtggg ggagagatac gcttttctct
     5401 gcgggctttt cagctcggcc accgtttcag aaccgcaacg gtgagggagc ggcccattgc
     5461 ggtgtggcgg ctcggtgatc cggcctgtcc ttgatccact tgcgggaacc agtcaggggc
     5521 ctctcgtttg cgcccggggt tctgaacgag ctgttcagaa tgtcgcagac tggctcgaga
     5581 gggtcgagtt gccggttgct gacgcggtcc cagtccgcag atacaatggc cggtgccggt
     5641 ccgagactgg tcgatggtcg ccgtgagctg cggtcatttc tcgcggtcgg tggtcggatt
     5701 gttgcgggag tcagatctgt gagcggtttt gttcagtcgg ttttggcagt gcgcatgcct
     5761 cagatcaacg ttaatgacga agaggtgact ctgatcggct ggcacgtcgt cgacggtcag
     5821 gaagtcggcg cagaccagcc gttgtgtgag gttgaaacga gcaaggccgt cggtgacgtg
     5881 ccgtccccgg cgtcaggcgt agtgaggctg gctgctgagg tcggcgatgt catcccgatt
     5941 ggccaggtgt ttgcctacgt ggggccatcc atagaggcta tcgacgcttt catcgccgca
     6001 cggggcgatg cttccacacc atcggtcgcg aggcgggcgg ctgaatcatc gcgtgtcggc
     6061 ggggttgacg cgacggtcgg ggcgattgaa ctggcccgca ggtacgggct tgatctggct
     6121 gcggtttccg cctccgggcg gattcgccga tcggatgtcg agcgattcat tgccgagaaa
     6181 gggttggccg caccggtcgg ccgagcggca actacccggg atgtcgtcaa tctgccgccg
     6241 tcgctcaagg acaaggtcgt cgatcagggg gcgttgccgg accatcttcg atcgattggc
     6301 gagcacctgg cccagaccca atcgcacctg gttgtggcac acgtggtgat ggacgcgtgt
     6361 gcgcggggag tggccgactg gatcgactcg cggcgcaagg ccggtgtgat gaccggtccg
     6421 atcccgatcc ttctgtacgc tgccgccgct gcaatcgccg tcgagcccag gcttcggcag
     6481 ttccgtcttg gcggacgcgc gtattcgtat cgtgacatcg atattgcatt tgccgcccga
     6541 agcggggatg gccggttgtt cacgcccgtg gtgcgcgggg tcgatcgtct tggtctcgat
     6601 gatctagcga gagagtgtgc ccgcctgtgc atggccgttt ttcgagggaa tcttgcggtc
     6661 gcggaaatgt ccggggcctg catgacggtt tcggtgctca gcgagcatcc ggttcgcttt
     6721 catgtcggtc tgcagaacgt ctggcaatcc gcaatcctga cggcgggggc gatacacgat
     6781 gaggttcgca tggatgacgg ccgacccatc tgtgtgccgg tgatgactct cacgctctcg
     6841 tatgatcacg gattgatgga tggatgggag gccgcggggg ctctcaacga ggccaagtcg
     6901 tgcatcgagg cgataggccc ctgatgtttc tgccgctgcc ggcatgttgt tcaggccgca
     6961 tggcatatgg ctgacgatgt ttcgcgatca tctctgaaac cccggccggt ccgcgccgaa
     7021 gacgcagtct cgcttcacag gctgctggtg gctgagttgc ccgatatccc ggccgacttc
     7081 ccgcggtggc ttgccaggtg gaactggcag tatcgtgaca accctttccg tcgggaccgg
     7141 ccggcgggat gggttctggc ggacggcgag caggtggtgg ggcacttggg cgcggtatat
     7201 gtacccgtgg gggcacgggg acggttcgtc attggcgcga tcggggcgga ttacgttgtg
     7261 tcgcaggatg cgctgaaggc cggcggtacg ttcgtcggcc ttcagcttgc ccaggcgttt
     7321 ttcgacggca ccagcggctg cctgcggatg gcgacaacgg ccaacgagaa gaccggtgcg
     7381 gttttcggcc ggttcggctg ccgcccggcg gagtggactc gggagttttt ccgcgcgccg
     7441 acaggactgg tgcaacaaat acgcacgtgc cggggcggag cgaatcgtat cgtgcgacgg
     7501 ctcatgatgg ggccgctggg ccttgtcggg aggagtgtgc tcgccgcggc atacggtctg
     7561 tgcgggcatc gaccggcttt gccgatcgcg cccggttgtc gactggacat cggcatggct
     7621 ccgctcattc tggaggctgc ctcgctgcac gggcggaccg cgccggttgg cttcggtatc
     7681 gcacgcagcc gggagtacct cgattggcgg tatgtgcggc atcctgagtc ggcgaacatc
     7741 cgtgcgttgc tggttcgacg ccctgagggc gaggtaatcg gggccgcagt ggtttttctc
     7801 gaccggcagg atggacggcg tatcgcctat cttgaggagc tgaccgtcgc gaccggcgac
     7861 gaagcggcaa tgcacactct ggtatgcgcc gccctcaggc tggcaggcga tcacaaaagc
     7921 cattatctcg tctgcaccgc cgggcgttcg gatcttcgcg ggcggttttg ggaatacggg
     7981 tttgaatccc gggcccgatc cgctccggcg ctggtcatcg gcggcctgga acaacaggcg
     8041 ggatccgcgt cggacgattc tttgatagct gcggccgtct ttcaccacgg cgacatgttc
     8101 tgattacttc cgcccggcgg cgacgaagta atagccgccg agatgtgcct caaccctcaa
     8161 gttggtgagt agctcggcca gcttcgtctc atatggccgt ccggtctcca cgtggttgag
     8221 ccacagccag cctcgggcaa gccttctcag cggtccccag cgatcgaaac ggccgaagtc
     8281 cagcaccacc attcgcccac cggggctgag gtgtccacat gcccgctcca aagcggactg
     8341 ccagtcgggg atcatcgaca gactgtaggc aaagagcacc gcatcgaatc gttcgttcag
     8401 ggctagccgc tcggcatcgg cgccaaccac cgtgaggttc acatacccgc gcgacgcgac
     8461 gcgcctggcc gcacggccga gcatgtgctg ggagaaatcc acacccacca gccgcccccg
     8521 ggctggatcg agacgtttga cgatgtgctc gaagttcagg ccggtcccgc aaccgatctc
     8581 gagcacgcgg ctgtcccgtt ggatttcgag caagtcaatt gcccgttgac gaccgtacag
     8641 gaacatccac cgggtcaggt catagacccc ggcctggtag cgatagaaac gttggacggg
     8701 atgtgtgggc accgacggcc tccggggtct tatcgcgctg tctgatcccg tcgcagtata
     8761 acctcacgct gacacgcacg ggcgccagag tcaacttgct caggcagctc tttcagaacc
     8821 gcgagcgtgc ttgccacgcc aaagcccggc gaaggcggga gggagcagcc gccctgcgat
     8881 gggtggcccg agtctggtgg acctgggatt gtccaacccc ctattggccg accgcaatct
     8941 ccagcgggtg gccggtcgag gcggggaagg tcacgtgagt tgggtccgtg gagtttgggg
     9001 ccgtc
//
LOCUS       DAOTPW010000236         8953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7080_length_8953_cov_5.375815, whole genome shotgun sequence.
ACCESSION   DAOTPW010000236 DAOTPW010000000
VERSION     DAOTPW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7080_length_8953_cov_5.375815"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="P5316_17665"
     CDS             complement(<1..779)
                     /locus_tag="P5316_17665"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR86839.1"
                     /translation="MRTTVLILAVLAGIARSCHGQAASGYDDYLEWQHWSRIQTGARA
                     GLASSYDRAGANADYSQYDDPPGLQTQPVPATVRTISGPGVIYRFWMPHLTAKQSFVV
                     RMFFDEETTPRIDTNSLALLGGGFGYFSAPLVTTCAGGQVCYEPIPFAQSLKIETVNQ
                     QLPTDGSWSARRHYYQYTYMTFPPGTDVRSYTGTLTPEQQAARDATAAMFNNAGRHPA
                     GTSPTAVRLDTTATTVSAGESITLACLAGPGVIRRLNVRMET"
     gene            complement(917..5890)
                     /locus_tag="P5316_17670"
     CDS             complement(917..5890)
                     /locus_tag="P5316_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86840.1"
                     /translation="MARRLNKQMVLGLTIASMILTTIAMVVVIKLLPQRDPKPAVLEA
                     ERLVRAGDFVEAVKKYQMAARRAKAMKDMDRYAEYMVLAGETALKAGSAIDARRCWSD
                     VMLNDPKNEAAQTRIVSLLLEFARYGDMQWSVLQTEAEKLVGINGNNAVGLHALGLAL
                     VQQRATNPRNAEDGEKQLIASIQADKGNPEYVNRLAQFYVTVGRHEDAERLYDQLIAE
                     LESQRAATPPETSPAQAVATTQPATGSSAEKLAEAYLMRGSFHTYKAERLHTQAMNAP
                     AAQAALLAAQSAEAEAKAKADLEKAIAVAPDNVEVLTGLARFWQTRRPPGQTEEEQKK
                     HREEYRALARKYYEQAIRINADTFDAYQGLARVYLAQEEPDKAIQVLKSRLNRGYNRK
                     HYLAGIDTIRMMNLREDCFKINMTRLYALLQKPSSADERDRAKKAVINEMTELYNQTV
                     ADTAAGEQHQWALFMKGRLLMVEDNINGAISKLEQAVKAFPSPPPELRQTLASLYLRT
                     RAFGPAVTQLELLVSESPSNAAAWTLLGEARLQAADPASPTYVTALTAASNAVKEALR
                     YDPNNRQALDTLARIYRAMGDEESANEIVSKMGISPEQLKLREAMITAMQAGSDEAKI
                     AEAEKMLKELLAADPLNTSALEILVRILSDRHARADESGKAGIAADIHKIINEARSAV
                     SRRIAAADSATQPAAERERYSNIAKAIERFAIAADPDMNDEEKIRGLEDLIRKGDDPY
                     QVAVQLFDIYRRMEKTKAQAAEQAAKILAMALEPDQGPVVEEIFLFATRNQDWSLAER
                     CIPLAARTGLDPTPNGHLYRGRLFLAQAGAGLDPQENAKKARDSLQLAVNAFPQHAMA
                     HVWLGFSHIGLKQHAEAKKALEVARQLDPRNGLALVGLGMVAESQGDFETLNNLLDAC
                     EKIAPQNPWVQARLAARREQMDPNQAIVQREATRKEKPQDVENLMALAALYQRVNRLD
                     DALKTYQECHALDPANLQAAERCATLLTQRREYDAAEKVLDKLAQSIDRNDAHQKATV
                     QLLRAALVGNKLRHQYGGIPASQQADLDNEFLEAAKMSDRPEVINNLVKYFRMTGRTN
                     EAVQWQRRLIEAVAKHFPQLDAQKEPRLALVDMLLQTGDVGRAPEIEKEISDFRGLFP
                     DDHRVHLLEGRLNAMKGDDARTIESFSNYIKNMPQDPVGYSYRGQTYLLIRRWNEGIR
                     DLQEAKRLDPAYQNYSLRVLLARAYNEIGQYESAINELQSILSESSNHLQAIETLVNT
                     YESKVVNRRTATDSLLAARAQADPQNPIWPAMRANVAMSRNQHGDAIRFAREAAEKSK
                     TADGQYDPARLDSFFRLCLQLGQFDELLAFVKDKMAPAQQALPIVHLFVASAHAGKHD
                     AEKAIEHYLASIQDGHIDLGLATGMILGDITSGRLTADAAAAAATKRADAEPDNPMAK
                     LLLLLVQRDQREPSVSIQAFRELADTIKPDNARNKTVLLWLKAQMADLAHQKTRQYNE
                     AVKLYEEMIQLEPRHTVARNNLAYLLMTYLNDPQAALPHAEAAVQTAPDNGSVIDTLG
                     WCQLLLKNYDEAVARLRQATQLEPEVASVRYHAAEAFYQRALADGAKSREADLEAAKT
                     EVQRAYELIVKNGKDPENIIGKVIELGGKLGLSLSPPTSAPGTSPQS"
     gene            complement(5979..6809)
                     /locus_tag="P5316_17675"
     CDS             complement(5979..6809)
                     /locus_tag="P5316_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86841.1"
                     /translation="MAGPSQTPVDASGWHSPTAILREALRNRGFVACVAMLALFAGGF
                     QLLAAVRGFIFVKQRVDLKTPLNLLKQERLAPYKLLQPIEIQSDILNQLGTDQYVHWI
                     LQVPEKPEQPASGRIFSLFVTYYTGLPDAVPHAPEACYAGGGHKLIKQTPDEVTITSQ
                     GQQIVVPVQVLEFEKESQFGRNSRIVLYTFHANGQFRPDRTAVRLAIGSLTDRYAYFS
                     KVEVGVDLDGRQFTKEQAVALAKEFLRVAMPVLLEDHWPDWEAVNRRSATQPTAGNGR
                     "
     gene            7197..7940
                     /locus_tag="P5316_17680"
     CDS             7197..7940
                     /locus_tag="P5316_17680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HRR86842.1"
                     /translation="MDRVFLHDRKPCVIVGAGGHGRVVLDILIRARQHKVIGFLDSNP
                     AIHGRRMDGVPILGDMSALPDLKRQGVAGAIVAIGDNGVRRGFADEIEAMGIDLINAV
                     HPSANLAHNVRIGRNVVIAAGALVCAHCQIGDSVILNTGCIVDHESMIGTAAHICPGA
                     RLAGRVTVEAGAFVGIGATVLQGLRIGCEAIVGGGAVVIRDVEPMTTVVGVPARSIRE
                     VPGYEEFAEVMTPAPLRGLPTTPVGAGRP"
     gene            complement(8106..>8953)
                     /locus_tag="P5316_17685"
     CDS             complement(8106..>8953)
                     /locus_tag="P5316_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86843.1"
                     /translation="FDDRHGPSWPDWNTTVINDLTPPTINAAAIHDAGDGNWIVQAHV
                     SDAHVAFLYYGDDGTGAPKVMRLGKNGDNYTATISGPAGDAYVIAQDVYFNTARANIV
                     NLPVIGLSTGTINRSAHVGQNVTPGDTFTVRNLGAETLTYSIEVEVETDQHWLSVMPT
                     TGSSTGEEDPITVSYDCATLPPGTYAGTIKVTGNAINSPKTIIVTVVIETVSPDFNHD
                     GDVDQEDFGHLQACLSGGAAYPTGCGNADLDGNGFVDNADCIIFQRCLSGENVIADGT
                     CDDQR"
BASE COUNT         1472 a         2586 c         2863 g         2032 t
ORIGIN      
        1 gtttccatcc gcacgttgag ccggcgaatt acgcccggac cggccaaaca ggccagcgta
       61 atcgactcgc cggcagaaac agtcgtggcc gtcgtgtcga gccgcaccgc cgtggggctg
      121 gtcccggccg gatgccggcc tgcattgttg aacatcgccg ccgtcgcatc gcgggccgcc
      181 tgctgttcgg gcgtcagcgt gccggtgtac gagcggacgt cagtgcccgg cgggaaggtc
      241 atataggtgt actgatagta gtgccgcctg gccgaccagg atccatcggt aggtaattgc
      301 tggttgaccg tctcgatctt caatgactgg gcaaagggga tcggttcgta gcagacctgc
      361 ccgccggcac atgtggtgac aagaggcgcg gaaaagtacc cgaaacctcc gccgagcagg
      421 gccaggctgt tcgtgtctat gcggggcgtc gtctcttcgt caaagaacat gcgtacgacg
      481 aacgattgct tggcggtcag gtgaggcatc cagaagcggt agatcacgcc agggccgctg
      541 atcgtcctga ccgtggccgg aaccggctgg gtctggagcc ccggtgggtc gtcgtactga
      601 ctgtagtcgg cattagctcc ggcgcggtcg tagctggagg caagtcctgc gcgcgctccg
      661 gtctgaatcc ggctccagtg ttgccactcc aggtagtcat cgtaacccga ggcggcctgt
      721 ccatggcaag aacgggcaat acccgccaac accgccagga tcaataccgt ggttctcatg
      781 tttttcgtcc ccggttgccg aggcctttcc ctgagcggcc gacgaaagat gcgtcaacaa
      841 ggcgttctcg atgcttcgcc ccattgccct gcgaggtaag gggacggagg gcgatcgttt
      901 gctgcggccg cggcggtcaa gattgcggcg aggttcccgg agcgctggtc ggcgggctca
      961 gcgaaaggcc cagtttgccg cccagctcga tcaccttgcc gatgatgttc tccgggtctt
     1021 tcccgttctt gacgatcagt tcgtaggctc gctgcacttc ggtcttggcc gcttcgaggt
     1081 cagcttcacg gctcttggcg ccatcggcca atgcccgctg atagaaagcc tcggccgcgt
     1141 ggtaacggac cgatgcgacc tccggctcaa gctgcgttgc ctgtcgcagg cgcgccactg
     1201 cctcgtcgta gttcttgagc aacagttggc accatcccag ggtgtcgata acactgccgt
     1261 tgtcgggggc ggtttggacg gccgcttcgg cgtgaggcag tgcggcctgg gggtcgttca
     1321 aatacgtcat gagcaggtac gccagattgt tgcgcgcaac cgtgtgcctc ggctccagtt
     1381 gaatcatttc ttcgtatagc ttcacggcct cgttgtattg ccgggttttc tgatgagcga
     1441 ggtccgccat ctgagccttc agccacaaca acacggtctt gttcctggca ttgtcgggtt
     1501 tgatggtgtc ggccaactcg cggaaagcct ggatggacac ggagggttcg cgctgatccc
     1561 gctggaccag gagaaggagc agcttggcca tgggattgtc gggttcggcg tctgcccgtt
     1621 tcgtggccgc ggctgcggcg gcatcggccg taagacgtcc actggtgatg tcgccgagga
     1681 tcatgccggt ggccagaccg aggtcgatgt ggccgtcctg gatcgaggcc agataatgtt
     1741 caattgcctt ctcggcgtca tgcttgccgg catgggcgct ggccacgaat aggtgcacga
     1801 tcggcagggc ctgctgcgct ggggccatct tgtccttgac gaaagccagc agttcatcga
     1861 attgacccag ttggagacag agcctgaaga aggagtccaa tctcgcgggg tcgtattggc
     1921 cgtcggccgt cttgcttttc tctgcggcct ctcgcgcgaa acgaatggcg tcgccatgct
     1981 ggttgcgact catggccacg ttggcccgca tcgccggcca gatcgggttc tgaggatccg
     2041 cctgggctct ggctgccagc agactatccg tcgcggtcct gcgattgacc acctttgact
     2101 cgtaggtgtt caccagcgtc tcgatggcct gaaggtggtt gctgctttcc gaaagaatgg
     2161 actgaagctc gttgattgcc gattcgtact gtccgatctc gttataagcc cgagccagca
     2221 ggacccgcag cgagtagttc tgataggcgg ggtcgagccg ctttgcctcc tggagatccc
     2281 tgatgccctc gttccatcgc cggatcagca gataggtttg gccgcggtac gaataaccga
     2341 cggggtcctg cggcatgttc ttgatgtagt tgctgaacga ctcgatggtc ctggcgtcgt
     2401 cgcctttcat ggcattgagc cggccctcga gcagatgcac ccggtgatcg tccgggaaca
     2461 gtccccggaa gtcgctgatt tccttctcga tttccggagc gcgcccgacg tcgccggtct
     2521 ggagcagcat gtcaacgagc gccaggcgag gttctttctg ggcatccaat tgcgggaagt
     2581 gcttggcgac ggcctcaatc aatcgacgct gccactggac ggcttcattg gtgcgaccgg
     2641 tcattcggaa gtacttcacc agattgttga tgacctccgg gcgatcgctc attttcgccg
     2701 cttcgaggaa ttcgttgtcg aggtcggcct gctggcttgc ggggatgccg ccgtactggt
     2761 gtctgagttt gttgccgacc aaagccgctc gcaggagctg caccgtcgct ttctgatggg
     2821 cgtcgttccg gtcgattgac tgggcaagtt tgtcgagcac cttttcggcc gcgtcgtatt
     2881 ccctgcgttg ggtcagcagg gtcgcgcaac gctcggctgc ctgcaggttg gcgggatcga
     2941 gggcgtggca ttcctgatag gttttcaagg cgtcgtccag cctgttcacc cgctggtaga
     3001 gggcggccaa ggccatgaga ttctcgacgt cctggggttt ttccttgcgt gtcgcctcac
     3061 gttggacgat cgcttggttg ggatccatct gctctcggcg agcggcgagc cgggcctgca
     3121 cccacgggtt ctgcggggcg atcttctcgc aggcgtcgag gaggttgttc aacgtctcga
     3181 aatcgccttg ggattcggcg accatgccca accccacgag cgcaagaccg tttcgcggat
     3241 cgagttgcct ggcgacctcg agcgccttct tggcttccgc gtgctgcttc agtcctatgt
     3301 gtgagaagcc gagccagacg tgggccatcg catgctgcgg gaaggcgttc accgcaagct
     3361 ggagcgagtc tcttgctttc tttgcgtttt cctgcggatc caagccggca ccggcctggg
     3421 ccaggaacaa gcggcctcgg tagagatgcc cgttaggggt tggatccagg ccggtcctgg
     3481 cggccaaggg gatacatctc tcggccagcg accagtcctg gtttctagtg gcgaacagga
     3541 atatctcctc aacgacgggc ccttggtcgg gctccaaagc catcgccagt atcttggccg
     3601 cttgttcggc cgcttgcgcc ttggttttct ccatgcggcg atagatgtcg aacaattgga
     3661 ccgcaacctg gtaggggtcg tcccctttcc tgatgaggtc ttccaggccg cggatctttt
     3721 cctcgtcgtt catgtcggga tctgcggcga tggcgaaccg ctcgatggct tttgcgatgt
     3781 tcgagtagcg ttcgcgttcg gcagccggtt gagttgccga gtccgccgcg gctattcttc
     3841 tggacacggc ggaacgcgcc tcattgatga tcttgtgaat atcggccgca attccggctt
     3901 tgccggactc atccgcccga gcgtggcgat cgctcaggat tctgacaagg atctcaagcg
     3961 cggaggtgtt caggggatcc gccgccagca gttccttcaa catcttttcc gcttccgcga
     4021 tcttggcctc gtcgctgccg gcctgcatgg ccgtgatcat tgcctcgcgg agtttcaact
     4081 gctccggcga gattcccatt ttggagacga tttcgttggc gctttcttca tcacccatcg
     4141 cccggtagat ccgggcgagc gtatcaagtg cctggcgatt gttggggtcg tacctgagcg
     4201 cctctttgac cgcgttggag gccgccgtga gagcggttac gtatgtcggg ctcgcgggat
     4261 ccgcggcttg aagtcgtgcc tcgccgagca gggtccatgc agcggcattg gacggcgact
     4321 cgctgaccag aagctccaat tgggttacgg cggggccgaa ggcccgcgtt cgcagataca
     4381 agctggccag ggtctgccgc aattccgggg gaggggacgg gaaggccttg accgcctgct
     4441 caagcttgct gatggctccg ttgatgttat cctcgaccat cagcaagcgt cctttcatga
     4501 acaatgccca ctggtgttgc tctccggcgg ccgtgtccgc cacggtctgg ttgtacaatt
     4561 cggtcatctc gttgatgacg gccttcttcg cccggtcccg ttcgtcggcc gacgacggtt
     4621 tttgcagcag cgcgtacaac ctcgtcatgt tgatcttgaa acaatcctct cgcaggttca
     4681 tcatccggat cgtgtcgatg ccggcgaggt aatgtttgcg attgtaacca cgattcagac
     4741 ggctcttgag gacctgaatg gctttatccg gttcttcttg tgcgagatag acgcgagcga
     4801 ggccttggta agcgtcgaac gtgtcggcgt taatccggat cgcctgctcg tagtatttcc
     4861 tggcgagggc ccggtattcc tcgcgatgtt tcttctgctc ttcctccgtt tgtccggggg
     4921 ggcgtctggt ttgccagaat cgcgccaggc cggtgaggac ctcgacgttg tccggtgcga
     4981 cggcgatcgc tttttccaga tcagccttag ccttggcctc ggcctccgcc gactgggctg
     5041 cgagaagggc cgcctgagcg gccggggcgt tcatggcttg cgtatgcagt cgttcggcct
     5101 tgtaggtatg gaatgaacca cgcatcaggt aggcttcggc aagcttctcg gcgctgctgc
     5161 ccgttgccgg ttgcgttgtg gcgacggcct gggcgggcga cgtttcagga ggagtggcgg
     5221 ctcgctgact ttccagttcg gcaatgagct gatcgtacag tctttcggca tcctcatgcc
     5281 ggccaaccgt gacgtagaac tgcgcgagcc tgttcacgta ctcggggttt cccttgtcgg
     5341 cctgtatgga cgcgatgagt tgcttttcgc cgtcttcggc gttgcgcggg ttggttgccc
     5401 gttgctgaac cagagccaat cccagggcgt gcagcccgac ggcgttgttg ccgttgattc
     5461 caaccagctt ctcggcttcg gtctgcaaga cggaccattg catgtcacca tacctggcga
     5521 actcgagcag gaggctgacg atgcgtgttt gggcggcctc gttcttggga tcattgagca
     5581 tcacatccga ccaacagcgg cgagcatcga tggctgagcc ggccttgaga gccgtttctc
     5641 ctgccaggac catgtattcg gcataccggt ccatgtcttt catggctttg gcgcgccttg
     5701 cggccatctg gtatttcttg accgcctcga cgaagtcgcc ggcccgcacc agccgttcgg
     5761 cttcgaggac tgcgggtttc gggtctctct ggggcaggag cttgatcacc accaccatcg
     5821 caatggtggt caggatcatt gatgcgatcg tcagtcccag caccatctgt ttgttgagtc
     5881 ttcttgccat gtctggtgtc tcttcctttg tgtccgagca acaagcgatt gcaggcttgt
     5941 cagcctattc agggcgttcg agcttcgcgg caggcggcct atctgccgtt gccggccgtc
     6001 ggctgggttg cggaacgtcg gttgacggct tcccaatccg gccaatgatc ctccaagagc
     6061 accggcatgg caacccggag gaactccttg gccaacgcca cggcttgttc tttcgtgaat
     6121 tgccttccgt ccagatccac ccctacttcg accttgctga aataggcata ccggtcggtc
     6181 aggctgccga tggccagtcg gaccgcagtt cgatccggtc ggaattgtcc gttggcatgg
     6241 aaggtgtaaa gcacaatccg ggaattccgc ccgaactggc tttccttctc aaactcgagc
     6301 acttgtacgg gtaccacgat ctgttgtccc tgacttgtga tcgtgacctc gtcgggggtt
     6361 tgtttgatca acttgtgtcc gccacccgcg tagcaggctt caggggcatg tgggaccgcg
     6421 tccggaaggc cggtgtagta cgtcacaaag agggaaaaga tccgcccgct ggccggctgt
     6481 tccggcttct ccggtacctg aaggatccag tgaacatact gatccgtccc caactggttg
     6541 aggatgtcgg attggatctc gatgggctgg agcagcttgt agggagccag tcgttcctgc
     6601 ttgagcagat tcaagggggt tttgagatcg acccgttgtt ttacgaatat gaagccgcgc
     6661 acggccgcga ggagctgaaa gccgccagca aataaggcaa gcatcgcgac gcaggcaacg
     6721 aacccccggt ttcgcagtgc ctccctcaag atggctgtgg gagagtgcca cccactggca
     6781 tcaactggcg tttggcttgg tccagccatg atggaacacc tttctgcagt ctgccaactt
     6841 gcggatcaac ccgcaacggg cgggatccac gattctcaag acccttatag acgcgaaggt
     6901 tacggctggt caacaagccc tcctttcgtc tcgtcaaggt ctttcgcaga ttcgtatgcg
     6961 cccccaagca cgtcgcaatg ccagagacca gaacagggca cagaaggcat tgagacggca
     7021 atccgtctct agtcgatgag ggcatttgcc ttacggtggc gtaagtatcg gctgagattt
     7081 gagcagcctt tcccccagtt ggctagagaa tcgtttcctt cgtggtctcg ccaatagtac
     7141 gaagccgata aggacagtgt tcagcctggc gttgggtcgt ccgataaagg tgagtgatgg
     7201 accgagtttt tctccatgat cgcaagcctt gtgtgattgt cggggccggt ggccacgggc
     7261 gtgtggtcct ggacatactc atccgggccc ggcagcacaa ggtaattggc ttcctcgact
     7321 cgaatccggc gattcacggt cggcgaatgg atggggtccc cattctgggg gacatgtcgg
     7381 cgttgcctga tctcaagcgt cagggggtgg cgggggcaat cgtggcgatc ggcgataacg
     7441 gcgtgcgtcg gggcttcgcc gacgaaatcg aggcgatggg aatcgatctg atcaacgcgg
     7501 tgcacccgtc ggccaacctg gctcacaacg tccggatcgg gcgcaacgtg gtgattgccg
     7561 ccggggctct ggtttgcgcc cattgccaga tcggcgactc ggtcattctg aacaccggct
     7621 gcatcgtgga tcacgagtcg atgatcggaa ccgccgccca tatttgtccc ggcgcccggc
     7681 tggcggggcg cgtgacggtc gaggcggggg cttttgtggg cataggggcg accgttctgc
     7741 agggacttcg aatcggttgc gaggccatcg ttggcggggg ggcggtagtc attcgtgacg
     7801 tcgagccgat gaccacggtg gtaggcgtgc cggcccgcag catccgcgag gttcccggtt
     7861 acgaggagtt cgcggaagtg atgacgccgg cgccattgcg aggtctcccg acgacacccg
     7921 ttggggccgg acggccctga aacctattcc ctggttccgt caactgcaga aagatcgcgt
     7981 ttgactcggc tcaagcggtc gcggcgcacg ccgccgatcc atcagatgcc gtttattctt
     8041 gtttcgccca gacaggtcgt gttgaaccgg cgtttggagc aaccagggag acccacgggc
     8101 actcatcacc tctggtcgtc gcaggtcccg tcagcgatga cgttctcacc cgacaggcat
     8161 cgctggaaga taatgcaatc tgcgttgtca acaaagccat tgccatccag atcggcattt
     8221 ccacagccgg tcgggtaggc cgccccgccg ctgaggcaag cttggagatg gccgaagtcc
     8281 tcctggtcca cgtcgccatc gtggttgaaa tcgggcgaga ccgtctcgat gacgacggtc
     8341 acgatgatcg tcttcgggct gttgatggcg ttacccgtaa ccttgatcgt tccggcgtac
     8401 gttcctggcg gcagcgttgc gcagtcgtag gagacggtaa tcgggtcttc ctcgccggtg
     8461 gaggagccgg tggtcggcat cacgctgagc caatgctggt cggtttcaac ctcaacctcg
     8521 atcgagtagg tcagcgtttc ggcgccgagg ttccggacgg tgaaggtatc gcctggggtg
     8581 acgttctgtc ctacgtgggc ggaccggttg attgtgccgg tgctgagacc gatcaccggc
     8641 aagttgacga tattggcccg ggcggtgttg aaatagacat cctgggcgat gacgtaggca
     8701 tcgcctgcgg ggccgctgat ggtggccgtg tagttatctc cgttcttccc caggcgcatg
     8761 actttcggtg ccccggtccc gtcgtcgccg tagtaaagga aagcgacgtg ggcgtcgctg
     8821 acgtgggctt ggacgatcca gttgccgtcg ccggcgtcgt ggatggcggc ggcgttgatc
     8881 gtcggcgggg tgagatcgtt gatgaccgtg gtattccagt cgggccagga agggccgtgc
     8941 cgatcgtcaa aca
//
LOCUS       DAOTPW010000237         8947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7091_length_8947_cov_4.993140, whole genome shotgun sequence.
ACCESSION   DAOTPW010000237 DAOTPW010000000
VERSION     DAOTPW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7091_length_8947_cov_4.993140"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /locus_tag="P5316_17690"
     CDS             <1..1903
                     /locus_tag="P5316_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86844.1"
                     /translation="RSLGVTSWAGQITLASIPPTAVLIAVLHVLGLYSLTTSSRCVIP
                     TVILPSFAIALALLLPSWRLGLSADRRGTTAHQTNRAAICRPGHTPHGRIRALLIPLL
                     AVAGSHAVFLTDAVSRYPTGCDALNYHLPAAARWVQDGQLSLNVDDWRLSMAANGELP
                     SMLLLSGGFERLLPILPVPFGLLAATTVYELTRRWGGDRRGAICAALLVLGAPIVIHH
                     MYVNYVDLFGTSIWLTSILAITWAQRLSGRNRIRLWVIAGLAAGIAAGTRLTFQVFAA
                     LSGVVLVASVVTVRQPARIGEAARAAVWFSLATAICCWFWPLRGALMMGNALHPIDHR
                     DGQLTIGNVPIDQIYGRSAAGEWLASRPLPARLLAALEMPWREHQYGTGYSYSVDGGT
                     GAAYAALVPVGLLSLIVRAVRKRATSAATRWHLLAGGLLAAGFLLHVTVFRMYTRYSL
                     GFVVLAIPLAAVWSGRLIAAWPRAVPVMLGLAFGITGGMAAIEPARDFAGRLIHRQWS
                     RSAYYGIPTMLDDLPAGTVVVNAGGTPNNYALLGRHLTNRVITAVEWQTWIQSGHPMQ
                     DLLRMNHVGYLYVSGRLPPARGAAPPYWPADLPVSCVFETRRGGSVPDNMITRVFRVE
                     PGIHGVAVR"
     gene            2061..3305
                     /locus_tag="P5316_17695"
     CDS             2061..3305
                     /locus_tag="P5316_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR86845.1"
                     /translation="MKLSIVIPAYNEEAAIGRTLERCLEAIEIIKAKSPIDAVELIVV
                     SDGSIDQTAAIAKSFENVRVIEFESNRGYGAAIKAGFEAANGDLLGFLDADGTCDPVQ
                     FAPLCRALVARNADVAIGARMGRDSRMPLVRMIGNRFYAFLLGVLACKPITDTASGMR
                     VVRASSLGDLYPLPDGMHFTPAMSARILMDDELKLVEVPMPYEERVGQSKLRLLRDGL
                     RFALTIIDAALVARPTRVILPVLAFLLLLVCAMTVRPLHEYVMTGTIQNWMIYRAVTA
                     LLIVTVGMLLLSAAVIADEIITIALKRPPRKSRLQILIARVFQTPFVAGLGTFSLGSS
                     LLLTRDGLWDYIVTGHVYIHWWRVMVSAALLIMAASAATTWLLMRIMSLLKKRQTAQP
                     SAIVSAEPVEAWQAKQLALEMD"
     gene            3321..4061
                     /locus_tag="P5316_17700"
     CDS             3321..4061
                     /locus_tag="P5316_17700"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR86846.1"
                     /translation="MTTCAHAGPDSLLLRLTGAVHRRCAHSRRVRVLAEVLSGLGITA
                     GRWLDIGCGDGQLAQAIMTAHRGVEIVGVDIQPRCDAVIPVERIDGRRLDYPDGFFDG
                     AILVDVLHHTDDPAAILTEAVRVSRTIIIKDHLCRGTVDRLTLSFMDWFGNAPSGVNC
                     PGHYLSTEQWIALFRQTRLHLDVLCTKLGLYPAAFNFLFERGLHFAARLTRVDCRPGL
                     ESKRFAIIERHGNQQQEGQYCLPSRITG"
     gene            complement(4051..8334)
                     /locus_tag="P5316_17705"
     CDS             complement(4051..8334)
                     /locus_tag="P5316_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86847.1"
                     /translation="MMTFLAVAAVMPGVVLAAGNGLRAHEGPNSAGIQLIDGGRSACA
                     IIYPAEGEAWRKIAARLAETIASRGAPEVPILPDTQAVPERLGPIRKDLQDLSLIVVG
                     DLNSNRAVFPYYANYYTCCDARYPGAGGYELRTIVRPFGGEKSCLIVGAADVADGEAA
                     VERLTEIIKGVKAPDHVELPCVLEVKPGNEVAALFQPAVEWLRRGGGSRPFEQHTPYS
                     LVLDHFTYAAHLYFYTGEEDFARRAREAILEVVRREPETVRTGDYTMENLAVAWRRVC
                     VWPHFSPEERARVDRALYGTVTEHSSAWWRLSDDSKGIGCRHHTTGMLAWWTLIRVLQ
                     EVGHLDAAARERLQEWRGEAEAYLKGLKRHYADDQDDYQSIDSAQNTASYSLQTGDLA
                     WYRNGLADQAARKLLAIADNQGWYAGIQGYGEALPGWERFALNGGLLLGSCAFVYGDG
                     AYDEALRRLPSLANSWGSLQPAGIHQFAGSRPLGPARAGLNAFMDVLRLTPYRLDLLN
                     AGDFLHSPLMDGYAVTGLRAPQVGSDSAFDKVVIRGLLPESVYLLLQGFSGTSLSTID
                     LNSIIRYCDLGKLWLVHNTGRRSLYFKNGVHVSSGIDEDPLPAACELLDHAGFADVAM
                     LAGRLPDYRGTEWTRYLFVLSKQFTVVLDHLRINEPGRYLVCCNWRTPGFASLENATW
                     ESIQDEAVFTVTEAEPEVSWSRRSPVRDGATRPTTLRQRHVLDAKGGEELLLTNLLQV
                     SSRDRPAGRGIRRIAPNAVIVTSWPQNEPKTTDRSAPADACLLVVGGNGIDLGSLRTD
                     ATMLVLNSDGAWLMGGTSLQLRSETFTAEAGRIPAKGSWPREVASLLAALCHEEVPGK
                     QEPPHTPAPQETDAPAPGWTLKIPGSRGGLVDGVRLVAERNVNGPSILATDWILPLLR
                     AEPRLQPGTSGGFNLTPPESRKATRPAASSMLAEVTLAPLKDASFRLVLPERSTINEI
                     SLFGESAGEDAPPLTPAEMIVELTFSNDGFSEDVRTRTIRARRQPTHHALYKGHDYVF
                     ETYRLSGFNEDAREVRVRIADGPTGEMPLAEIHVRTVGACGVQPVQTRTIDLDGDGND
                     EILAWTPEGEFAVVRADGSLLWARSWAEGIIAVDAWDLEDDSRREIFVSRGDRRVDVL
                     NLDGSPRWHRDFSRMRQETGEQMYGDGSLVYGMAAWRPEGSAEKQVLCTSYFFSAILD
                     AHGRLQKCFRRAGHHAAIREVPSRFAADGQFAIRCDIPWVGPVPLEWWAGKADEAVAG
                     CSVHNGPLVHFEIDDFDADGKVEALVAGEEGVGLYGRAEPPVKWQHSTDAPCIGAAAV
                     KVDDRRPATIIYGREDGYVFVLGPDGREISSTLLDDPAMCLTALRASSGQAVILVGTR
                     ASLRCLKVDGLSEIWRRAGCYVRLELLSAGDTKRVLAVRPDGTMDVFNL"
     gene            8484..>8947
                     /locus_tag="P5316_17710"
     CDS             8484..>8947
                     /locus_tag="P5316_17710"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRR86848.1"
                     /translation="MIDFVQLRDLYKATLLDDVIPFWMHHAIDPNGGINTCIQDDGTL
                     LSRDRWGWSQWRAVWVFSKLYNAIEQRMEWLRTAEGICSFLSKQGPLEDGHWPLLMDG
                     DGQVRRGFESIYTDGFAIYGLVELWRANRKQSCLDLAMSTFAATEKTLGRNDP"
BASE COUNT         1653 a         2749 c         2764 g         1781 t
ORIGIN      
        1 gaggagtctc ggtgtgacgt catgggccgg ccagattacc ctggcttcga ttcctcccac
       61 ggccgtgctg atcgccgtgc tccacgtgct tggtctgtac tccttaacca cgtcatcgcg
      121 gtgtgtcatt ccgacggtta ttctgccatc gttcgcaatc gcgcttgcac tgctgctgcc
      181 gtcgtggcga cttggtctgt ctgctgaccg gcgtggcact acagcccacc aaaccaatag
      241 ggccgccatt tgccgtcctg ggcacacgcc gcacggccgc atccgagcgc tgctcatccc
      301 gctgctggct gtggccggtt cacacgcggt gtttctcacc gacgcggtat cgcgatatcc
      361 caccggatgc gacgccctca actaccattt gccagcggcc gcacgatggg ttcaggacgg
      421 gcagttgtcc ctcaacgtcg atgactggcg tctatcgatg gccgccaacg gcgagctgcc
      481 ttcgatgctg ttattgtccg gcggcttcga gcggctgttg ccgatcctgc cggtcccttt
      541 cggcctgctg gctgctacaa ccgtatatga gctgacccgt cgctggggcg gagacaggcg
      601 gggagcgatt tgcgccgccc tgcttgttct tggcgcacct atcgtgatac atcacatgta
      661 cgtcaactat gttgatctgt tcggcaccag catctggttg acatcgatcc tggccatcac
      721 atgggcgcag cggctcagtg gaagaaaccg gatccgcctt tgggtgatcg ccggactcgc
      781 ggccgggatt gcggccggaa cgcgcctgac gtttcaggtc tttgcggcgc tgagcggggt
      841 tgttctggtc gcgtcagtcg ttacggtccg acagcctgcg aggataggcg aagccgcgcg
      901 cgccgccgtg tggttctcgc tggccacagc gatctgctgc tggttctggc cgctgcgggg
      961 ggccctgatg atgggcaacg cgttgcatcc gatcgaccac cgcgacggcc aactgacgat
     1021 cggcaacgtt cccatcgatc agatctatgg tcggagcgcg gccggcgaat ggctggcgtc
     1081 aaggccgttg cccgcgcggc tgcttgcggc cttggagatg ccgtggaggg aacaccagta
     1141 tggcacgggc tacagctaca gtgttgacgg ggggacgggg gctgcctatg ccgcgctggt
     1201 accggtcgga ctgctgtcgc tgatcgttcg agccgttcgg aaacgtgcta catccgccgc
     1261 gacgcggtgg catttgcttg ccggaggctt gctggccgcc ggatttctgc tgcacgtcac
     1321 cgtgttccgc atgtacactc gatattcgct gggttttgtt gtgttggcca tcccgctcgc
     1381 ggcagtttgg tccggtcgat tgatcgctgc ctggccgcgg gccgtgccgg tcatgttggg
     1441 tcttgccttc gggatcaccg gcgggatggc cgctatcgaa ccggcccgcg atttcgcggg
     1501 gcgattgatc catcgacaat ggagccggtc ggcatactat ggcattccga ccatgctcga
     1561 cgacctgccg gctggaacgg ttgtcgtgaa cgccggcggc actccgaaca attacgcgtt
     1621 gcttggcaga cacctcacga atcgggtcat caccgcggtc gaatggcaaa cgtggatcca
     1681 aagcggccac ccgatgcagg atctactgag gatgaaccac gtcgggtact tgtacgtatc
     1741 aggcagactt ccccctgccc gtggcgccgc gccgccctat tggccggcgg atctgccggt
     1801 ttcctgtgtc tttgaaacgc gtcgcggggg atctgtgccc gataacatga tcactcgcgt
     1861 ctttcgcgtc gagccaggta ttcacggcgt ggctgtccga taatcgcggc gatgaaaggg
     1921 ccggaccgtc tcatctgcct ctttgccgca gccccggagc ccgcgtcaaa ctcattcgga
     1981 ccatcggcag ggacgataaa cctcatacag gtctgcccca tggggatcac tagaacccgg
     2041 acctgaccgt gagaaagcgc atgaaactca gcatcgtcat ccccgcctac aacgaagagg
     2101 cggcgatcgg gcggactctc gagagatgtc tggaggccat tgagatcatc aaggccaaaa
     2161 gcccgatcga cgccgttgaa ctcatcgtcg tcagcgacgg atcaatcgat cagacggccg
     2221 cgatagccaa gtcattcgag aacgtgcgcg tcatcgagtt tgaatccaat cgcggatacg
     2281 gggcggcgat caaggccggc ttcgaggcgg caaacggtga tctgctcggt ttcctggacg
     2341 ccgacggcac ctgtgatccg gtgcagtttg ctcccctttg ccgagcatta gtcgcgagga
     2401 acgcagacgt tgccattggc gcccgcatgg gccgcgattc gcggatgccg ctggttcgca
     2461 tgatcggcaa tcgcttctac gcttttctgc tcggggttct ggcatgcaag ccgatcaccg
     2521 acacggccag cgggatgcga gtcgttcggg cctcctcgct aggcgacttg taccccttgc
     2581 ccgacggcat gcatttcact ccggcgatga gcgcccggat cctcatggat gacgaactga
     2641 agctcgttga ggtgccgatg ccctatgagg agcgcgtggg gcaatcgaag ctgcgactat
     2701 tgcgggacgg cctgcggttc gcgctcacca tcatcgacgc cgcgctcgtg gcccgtccaa
     2761 cacgtgtgat tcttccggtt ctggcgttcc tcctgctatt ggtctgcgcg atgacggttc
     2821 gccccttgca cgaatacgtc atgacgggaa ccatacagaa ctggatgatc taccgggcgg
     2881 taacggccct gctcattgtc accgtcggca tgctgctact gagcgccgcg gtcatcgccg
     2941 atgaaatcat caccatcgcc ctgaagcggc cgccgaggaa gtcgcgattg cagattctga
     3001 ttgcccgcgt ttttcagacg ccctttgtag ccgggctagg caccttcagc ctcggatcct
     3061 cgctgctcct gacccgcgac ggcctctggg actacatcgt gacggggcac gtgtacattc
     3121 attggtggcg ggtcatggtt tcagcagcac tgctgatcat ggcagccagc gcggcgacaa
     3181 cctggctcct gatgcggatc atgtcgctgt tgaagaagcg acaaacggcg caaccgtcgg
     3241 ccattgtctc agccgaaccg gttgaggcat ggcaggccaa gcaactcgcg ctggagatgg
     3301 attgaccgca gggtgccgcc atgaccacgt gcgcacacgc cggacctgat tcgttgcttt
     3361 tgaggctgac cggggccgtt catcggcgat gcgcccattc gcggcgagtt cgagtcctcg
     3421 ccgaggtcct atccggactc ggcatcacag ccggccgatg gctggatatc ggctgcggcg
     3481 acgggcaact ggcccaggcg atcatgacgg cccatcgggg cgtggagatc gtgggcgtgg
     3541 atatccaacc gcgctgcgac gcggtcattc cggtcgagag gattgacggc agacggctcg
     3601 actatccgga cggattcttc gacggcgcca ttctcgtcga tgtgctgcat cacacggatg
     3661 acccggctgc gatcctgacc gaagccgtcc gcgtcagccg cacgatcatc atcaaggacc
     3721 atctttgtcg tggcaccgtg gaccggctga ctctgtcttt catggattgg ttcggaaacg
     3781 ccccatcagg ggtcaattgc ccgggccact acttgagcac ggaacaatgg atcgcccttt
     3841 tccgccagac acggctacac ctcgatgtgc tctgcaccaa gctgggcctc tatccggcgg
     3901 cgttcaactt tctttttgaa cggggcttgc acttcgcggc ccgtctgaca cgcgttgact
     3961 gccgaccggg cctcgaatca aaacggttcg cgatcatcga gcggcatgga aatcagcaac
     4021 aggaagggca gtattgcctg ccgagccgga ttacaggttg aaaacatcca tggttccgtc
     4081 gggtctgacg gcaaggactc gtttcgtatc tcccgctgag aggagctcca gtcgaacgta
     4141 gcaacccgct cgccgccaga tctcgcttaa tccatccacc ttcaggcatc gcagcgatgc
     4201 tcgcgtaccg acgaggatca cggcctgccc gcttgaggca cggagggcgg tgaggcacat
     4261 cgcggggtca tcaaggagtg tgctgctgat ttccctcccg tcggggccga gcacaaaaac
     4321 gtatccatcc tcgcgaccat agatgatcgt ggccggccgg cgatcgtcga ccttcacggc
     4381 cgccgcgccg atacaaggcg catcggtaga atgctgccat ttcaccggcg gctcggcccg
     4441 gccataaagc ccgacgccct cttcaccggc caccaacgct tccactttgc cgtcggcgtc
     4501 gaagtcgtct atctcgaaat gcacgagggg gccgttgtga acgctgcaac cggccacagc
     4561 ctcgtctgcc ttaccggccc accattcgag aggcacggga ccgacccatg gaatatcgca
     4621 acggatcgca aactggccgt cagccgcgaa tcgtgacggc acctcgcgga tggccgcgtg
     4681 gtgtcccgca cgtctgaagc acttctgcaa gcggccatgt gcgtcgagta tcgcggaaaa
     4741 gaaataggag gtgcaaagta cctgcttttc ggccgacccc tcgggtctcc acgccgccat
     4801 accgtagacc aggcttccat cgccgtacat ctgctcgcca gtttcctgtc gcatccgaga
     4861 gaagtcgcga tgccaacgag gcgaaccgtc cagattcagc acgtccacgc ggcgatcgcc
     4921 gcgactgacg aatatctccc ggcgactatc gtcctccagg tcccaggcgt caactgcaat
     4981 gatgccctca gcccagctcc tggcccatag taacgagccg tcggctcgca caaccgcgaa
     5041 ctctccctcg ggggtccagg ccaagatctc gtcgttgccg tcgccgtcga gatcgatggt
     5101 cctggtctgc acgggttgta ccccgcaggc accaaccgtg cgcacgtgga tttctgcgag
     5161 cggcatctcg ccggtcggac cgtcagcgat gcgcacccgg acttcgcggg catcctcatt
     5221 gaaaccggac agtcgatagg tctcgaacac ataatcatgg cccttataaa gggcgtggtg
     5281 agtgggttgc cgccgtgcac ggatggttcg ggtacgcaca tcctcgctga aaccatcatt
     5341 actgaaggtc aattccacga tcatttccgc cggagtcaga ggcggggcat cctcgcccgc
     5401 cgactcgccg aaaaggctga tctcgttgat tgtcgacctc tcaggaagca ccaatcgaaa
     5461 cgacgcgtcc ttcaatgggg caagcgtcac ctcggccagc attgaggaag ctgccggacg
     5521 cgttgctttg cgcgattcgg gaggcgtcag attgaatccg ccgctggtgc ccggctgcaa
     5581 tctcggttcg gccctgagca aagggaggat ccagtccgtc gccagtatac tcgggccgtt
     5641 gacgttgcgt tccgcgacaa gacgaacgcc atcaaccagt ccgccacgag agccggggat
     5701 tttgagcgtc cagcccggag cgggagcgtc tgtctcttgg ggtgcaggcg tatgcggcgg
     5761 ctcttgcttc ccaggaacct cctcgtgaca cagtgcggca agcagcgagg cgacttcgcg
     5821 tggccaggat cctttcgccg gaattcgtcc ggcctcggcc gtgaaggttt cggaacgcaa
     5881 ctgaagggac gttcctccca tgagccacgc gccgtcgcta ttaagcacga gcatggttgc
     5941 atcggtcctc agcgatccga ggtcgattcc atttccgccg acgacgagaa gacaggcgtc
     6001 ggcgggcgcg gaccggtcgg ttgtcttggg ttcattctgc ggccatgacg tgacaatcac
     6061 ggcgttgggg gcgatgcgtc ggatgccgcg gccggctggg cgatcgcggc tcgatacctg
     6121 aaggagattc gtgagaagga gctcttcgcc gcccttggca tcgagcacgt gacgttgccg
     6181 cagggtcgtc gggcgcgtgg cgccgtccct gaccggtgag cgccggctcc atgagacctc
     6241 cggctccgct tcggtcacgg tgaagacggc ctcatcctgg atggattccc acgtcgcgtt
     6301 ctcgagcgaa gcgaagcccg gagtgcgcca attacagcag accaaatagc ggccgggttc
     6361 gttgatccgg aggtgatcga gaaccacggt aaattgtttt gacaagacga acagatacct
     6421 ggtccattcg gtcccccggt aatcgggcaa ccggccggcc agcatcgcca catcggcgaa
     6481 gccggcgtga tcgagcaatt cacaggcagc ggggagcgga tcctcatcga tgccgctgga
     6541 tacgtgaacg ccgttcttga agtagagtga gcgacgtccg gtgttgtgaa caagccagag
     6601 cttgccaaga tcgcaataac ggatgatgga attcaagtcg atggtgctca gcgaggtacc
     6661 cgagaagccc tgcagcaaca agtagacgct ctcgggcagc aggccacgga tcaccacctt
     6721 gtcaaacgcc gagtcggagc cgacctgcgg cgcacgaagg ccggtcacgg cgtagccgtc
     6781 catcagcggg ctgtgaagaa agtcgccggc gttgagcaga tcgagtcgat acggagtcaa
     6841 ccgcaacacg tccatgaaag cgttgaggcc ggcccgggcc gggcccaagg ggcgagaacc
     6901 tgcaaactga tggataccgg ccggttggag tgagccccac gagttggcga gggaaggcag
     6961 ccgcctgagg gcttcatcat aggctccatc gccgtagaca aaggcacatg atcccagcag
     7021 aaggccgccg ttgagcgcga agcgttccca gccgggcagg gcctcgccat agccttgaat
     7081 gccggcgtac caaccctggt tgtcggcgat cgcgagcagt ttgcgggccg cctgatcggc
     7141 caagccgttg cgataccagg caaggtcgcc ggtctggagg ctgtaacttg cggtgttctg
     7201 agcggagtcg atcgattgat agtcgtcctg gtcgtcggcg tagtggcgtt tcaggccctt
     7261 aaggtatgcc tccgcctcgc ctcgccactc ctgcagtcgc tctcgtgccg cagcatcaag
     7321 atggcccact tcctgcaaca cgcggatcaa ggtccaccag gccagcatgc cggtggtatg
     7381 atgccggcat ccaattcctt tggagtcgtc gctgagacgc caccaagcac ttgagtgttc
     7441 agtcaccgtt ccgtaaagtg ctctgtcgac ccgcgcccgt tcctcgggcg agaaatgcgg
     7501 ccagacgcag acgcgcctcc aggcaactgc aaggttctcc attgtgtagt cgccggttcg
     7561 taccgtttcc ggctcgcggc ggacgacttc gagaatcgcc tcacgcgcgc gtcgtgcgaa
     7621 gtcttcctcg ccggtgtaga aataaagatg agccgcatac gtgaagtgat caagcacgag
     7681 cgagtatggc gtgtgctgct cgaacgggcg agagcccccg cctcttcgga gccactcgac
     7741 ggcgggctga aagagggcgg caacttcgtt gcccggtttc acttcgagaa cacaggggag
     7801 ttcgacgtgg tctggggcct tgacgccctt aatgatctcg gtcaatcgct caactgccgc
     7861 ctcaccatca gcaacatccg cggctccgac aataaggcag cttttttcgc cgccgaaagg
     7921 ccggacgatg gtcctcaatt cgtagccgcc ggcgccaggg taccgggcgt cgcaacaagt
     7981 gtaatagttg gcgtagtagg gaaaaacggc ccgattgctg ttgagatcgc ctactacaat
     8041 gaggctgaga tcctgcagat ccttccgtat gggtccaagt cgttcgggca cggcttgcgt
     8101 gtcgggcagg atggggactt cgggcgcccc acgtgaagcg atggtctcag ccagtcgggc
     8161 ggctatcttc cgccaagcct cgccttcagc cgggtatatg atcgcgcagg ccgatcggcc
     8221 gccgtctatc agttgaatac cggctgagtt tggcccctcg tgagccctca acccgttccc
     8281 ggcggctaag acgacaccgg gcatcacggc tgcaaccgcg agaaacgtca tcatgcagca
     8341 ttccccggac ggtttggtaa acggcgtcgc atcatgtatt cttttcttcc ccgcaatggc
     8401 cgagcattcg gctttgcgtc gggacaatca gcatagggta tcgtgttcga aaaaccaatt
     8461 gtgagaagcg gcaagtccaa accatgatcg acttcgttca actgcgtgat ctgtataagg
     8521 cgaccctgct tgatgatgtg atcccgttct ggatgcacca tgcgattgac cccaatggcg
     8581 gcatcaacac gtgcatccag gatgatggca ccctgctcag ccgtgatcgt tggggatggt
     8641 cgcagtggcg ggcggtgtgg gtgttcagca agctgtacaa cgccattgaa caaaggatgg
     8701 agtggctgag gacggctgag ggaatctgct cattcctttc gaaacaaggc ccgctggagg
     8761 atgggcattg gccgctgctg atggatggcg acgggcaagt ccggcggggg ttcgagagca
     8821 tctacactga cggctttgcc atatatgggc tcgtcgagct ttggcgggca aaccgcaagc
     8881 agtcctgcct ggacctggcg atgtcaacct ttgccgccac cgagaagacc ctcggtcgta
     8941 acgatcc
//
LOCUS       DAOTPW010000238         8915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7133_length_8915_cov_5.355079, whole genome shotgun sequence.
ACCESSION   DAOTPW010000238 DAOTPW010000000
VERSION     DAOTPW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7133_length_8915_cov_5.355079"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="P5316_17715"
     CDS             <1..1180
                     /locus_tag="P5316_17715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRR86849.1"
                     /translation="EMRLALTQTAPGLSAIVCQPALLAELAKAGQPLCRIMAYRPEEP
                     RKDLATQSAYYAVPAEISHAVGRCIADTQAALDRVAQILVETARDNQTPDATTASRWL
                     LDHRYDQIGVLPEEVEIELTTEDSLPNTTLRPRGSAVGRRGPMSMDMYKRLIDELATR
                     DDARIVLGGFGDPLLHPEWPAMAAYARQAGILGIAVRTTGINLDASAIDALMSPRVDV
                     LNVLIDAMTPATYRDVHRADCYQQVAANIEALCIANHQRRQPQPLIVCEMAKTRATIS
                     EMEKFFDHWIAKTGSVYVAGPSHYARQWPELAVMSMAPPARVPCYRIFRRTFVLADGR
                     VTVCDQDYRGNHAIGSLTDTSLSSLWTGPIMMAVRRSHLDHRFDGIPLCPLCEEWNRP
                     "
     gene            1523..2362
                     /locus_tag="P5316_17720"
     CDS             1523..2362
                     /locus_tag="P5316_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR86850.1"
                     /translation="MKIGCDLMLWTGCFTRKSIPLIERVAKLGFDGVEFPMFDPKAVD
                     VRATRKALEATGLGSTVCTVIVTGSPISPEARERQAAIDHVVRVLEVSKELGSDCVAG
                     PLYSPVGRLVGRGRNAAEWKYAVASLRKIADVAEILDINVGIEPLNRFETYFLNTCED
                     AAALCDEIGSTHVGVHYDTFHANIEEKDPTAALRKIGRKRLFHVHACENDRGIPGTGH
                     VPWKDFFKTLKQLKYNRWLTIESFVPAIKELAAAAAIWRPLAKDGDDLAKRGLRFLRA
                     NVG"
     gene            2482..3570
                     /locus_tag="P5316_17725"
     CDS             2482..3570
                     /locus_tag="P5316_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86851.1"
                     /translation="MVRRDMTITLVSGLTALAAASARGGPNLIRNPGFEEGESPTYPG
                     VGLHWETNDAQPHPDIDVLTTSTKYSGVHSQWLKAHPTWDLGMVRQVTPYGSISPGMI
                     YRVSAWIRTANVQNPAGWYVFGIWYLSNDTWIGESKMPRQEALNYDWREITWTVVPPE
                     GTNRIAVLLTRHTDGDAWYDDVSITQVSAGPPQISVSPGSFEIKAVKGRRIESGTLWV
                     QNVGGGILEYALTDEVRWLSCVPSAGTSTGEVDRIIIAYDIAGLSVGFYRASITVADP
                     AAVNPVVAVPVDLTVSTPGDLDYDGDVDQTDYGLFQACYSGNGIEAAETCTDATMDAD
                     ADVDLDDFSLFWLCLSGPGIEADEHCAD"
     gene            complement(3676..5913)
                     /locus_tag="P5316_17730"
     CDS             complement(3676..5913)
                     /locus_tag="P5316_17730"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRR86852.1"
                     /translation="MTSLPMTVQERIPMPMGPIIRPELAGTSITVQDVIRILKQRIFL
                     ILFIWILIFGLTIGGTYLWVKYWPGYTAMGQVVVESPLPKAPMEFTNPIVQVDLLDRA
                     VADQMVRLKSEGLLRDLITNDARVRETQWFKSGRDANERVQKLQSSLSVKQIPQTNYI
                     LVSLSTRNPEDAPVIINQLIDKYIFETTTGSQRSYREELDTYTREEEAIRARLQNLRD
                     QQADFLKMELTEPGAIKGINIVGETWRVLAQEAARIGTEKLQYQAQYENLVNRDPSQA
                     FITPQMRMMIEQDPEVVQLQNARVMQEQLLLARQQQGLGENHREIRELKSQMESIDNQ
                     LADARARKEQAVLEAEIAQAQTMYFNAMHAEQQLLEQIEQERSKQRDLDRQIVEYENR
                     EKEYELLQQQLERVSDYIAQLNMLIRTTSAIRLSGVRALVPLERSSPRWEYNLPAGFV
                     LGLLLGVGLAVLLEFIDTSLKTGRDVIRHVHVPVLGTVPDLDDEEIPIDAIELAPHVA
                     PRSMVAEAFRNIRTNLLLTCPADRQRTVLITSPRPEDGRTAVAVNLAISLAQSGRRVL
                     LVDANFHRPRLRTIFPKSNIDGLSNVLLGRTRLADVVSKTELPNLDVLTTGPIPPNPT
                     ELLAATYLQQVITQATERYDQVIFDGPPVLLVSDVLVMTPSVDGVILVFRAQNTSRGV
                     AQRAREQIERVEGHIFGAILNAAQVTRGGYFREQIRSYYDYQSEQLLAAESRRELPGD
                     QGKEA"
     gene            complement(5910..6833)
                     /locus_tag="P5316_17735"
     CDS             complement(5910..6833)
                     /locus_tag="P5316_17735"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HRR86853.1"
                     /translation="MSTKPRSLWTPANTLQAAALTTLLAVLFWHVLHRLVLKWLSSED
                     WSHGFIIPLFSLYYLYIRRDMMPVGLRDRSVLPRLAGAAFLTLAFLLYLKATIARIDY
                     PKNVALVMSIVGTLLMVCGWPLTRWSWFAVAFLMFAMPLPDNVYGQITSPLQRIAARV
                     SAGVLSMVPDMMTEATGTIVEYSYQNRTGTLDVERACSGMRLLMTMTALGVAMTFVNE
                     RALWQRLIMILVCVPIAVFCNIIRVTTTGFFVVFGRQDLAQGTAHTLLGLMMLAVAFS
                     LYGATSYVLNHLFVDHQPEPEDGTATGGIAG"
     gene            complement(6856..8508)
                     /locus_tag="P5316_17740"
     CDS             complement(6856..8508)
                     /locus_tag="P5316_17740"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRR86854.1"
                     /translation="MRLNPKYSPVRWGFGLAGTLLPVAFISGCASAWWNGFLDPTQVG
                     NFRENVVTEIQDTVSFEDKPSGIPNAVDPTPDDLVATVENYQLGPGDVFQITLLDFMA
                     RGAQSEFVLTVDDLGYVDIAQIGRIRVEGLTLPEVRDEVARQAKARGIYAEESEPTIT
                     VIMGDQQNRTFSITGAVQAPGTYRIPRPDFRLTEALIMAGGSDEMLQRLYAGGRVRTI
                     YVIRNERRPKTVKESVVKGKPSRDETNLKPADTGLPSAVVPEPAVPEPQSVQPSTRPG
                     TQGPALPTDSVEQDLIDAVAPEGASPRRGELPSSAPQSKNESPPPLRPFIFVNDKLIE
                     APAGQEARRAATSASTVVETVPQTEQATRPVDWSELAGEGQQRIIRISAEALRTGDSS
                     SNIVIRHQDLIRVDPGPIGTYYVGGHVVMPGQYPLNGEQVTLAQAVIAARGLDPLAWP
                     TRCEIRRRIDRDREQITQWDLARIMAGQDPDVFLRPNDVVNVGTHAIAPFLYTIRNAF
                     RFSYGFSFSYDRNWAEYDTLAGQYYRARTEASRRGLLSAFDF"
BASE COUNT         1750 a         2765 c         2658 g         1742 t
ORIGIN      
        1 cgagatgcgt ctggccctca cgcagaccgc cccgggccta tctgcgatcg tgtgccaacc
       61 cgccttgctc gccgaactgg ccaaggccgg gcaacctttg tgccgaatca tggcctaccg
      121 gccggaggag ccgcggaagg atctcgccac ccagagcgct tactatgccg tcccggccga
      181 gatcagccat gccgtcggcc gttgcatcgc cgacactcag gccgcactgg accgtgtcgc
      241 ccaaatcctg gtcgagaccg cccgggacaa ccagactcct gatgccacaa ccgcttcccg
      301 ctggctgctc gaccacaggt acgaccagat cggagtattg cccgaagaag tcgaaatcga
      361 gctgaccacc gaggactcac ttcccaacac gacgcttcgg ccgcgcggat cggccgtcgg
      421 ccgacgcggg ccgatgagca tggacatgta taagcggctg attgacgaac tggccacccg
      481 ggacgacgcg agaattgtcc tgggcggctt cggtgatccg ttgctgcacc ccgaatggcc
      541 cgcgatggcg gcttatgccc gccaagcggg gattctcggc atagccgtcc gaaccaccgg
      601 catcaacctg gatgcctcgg caatcgacgc gctcatgagc ccgcgcgtcg acgtgctcaa
      661 tgtcctcatc gacgccatga cccctgccac ctatcgcgat gttcaccgtg ccgactgtta
      721 tcaacaggtt gcggccaaca tcgaggcgct gtgtatcgcg aaccaccagc gccgacaacc
      781 tcagccgctc atcgtctgtg agatggccaa aacgagagcg acgatcagcg agatggagaa
      841 gttctttgac cactggatcg ccaagacggg ctcggtctat gtggccggtc ccagtcacta
      901 tgcccgccag tggccggaac tggcggtgat gtccatggcc ccgccggcaa gagtcccctg
      961 ttacaggatc ttccgccgca cgttcgtgct ggccgatggc cgggtcaccg tctgtgacca
     1021 ggattacaga ggcaaccatg ctatcggatc gctgacggac acctcgcttt cttccttgtg
     1081 gaccggcccg atcatgatgg ccgtccgccg ctcgcatctg gatcaccgct ttgatggcat
     1141 cccgctctgc ccgctctgcg aggaatggaa ccgaccgtag ccgtcgtgct ctggcacccg
     1201 gccggtacag aaggacgtcc gccattgccc tgacgaccgc cgtgagccct ggtccattcg
     1261 cgaatggttt ccggcatacc cggggcattg gcggcatcac cgttgagctc gtgagccggc
     1321 ttcgccgtcg gggagcacga tgaccacgag aggccacatg ccgagaactg tcacatcact
     1381 gaccggccgg gtatgatgtt cgcaacccgg cgggccccgt gcgccaacat tgacggccgg
     1441 ctgatggagt attggcgggt tgaacggctc gtcgactgtc tcaacccttt tcagaagacc
     1501 agacaatgtc aggaggttcg acatgaagat cggttgtgac ctgatgctgt ggacgggctg
     1561 cttcacacgc aagagcatcc ccttgatcga gagggtggcg aagctgggat tcgacggagt
     1621 ggagttcccg atgtttgacc ccaaggccgt ggatgttcgc gcaacgcgca aagccttgga
     1681 agccaccggc ctcggctcca cggtgtgtac cgtcatcgtg accggctcgc ccatcagccc
     1741 agaagcccgt gaacgccagg cagcgatcga ccatgttgtt cgcgtcctgg aggtgagcaa
     1801 ggaactcggc tccgattgtg tggccggtcc gctgtactcg ccggtcggca ggctcgttgg
     1861 ccgcgggcgc aacgccgccg aatggaaata cgccgtggca agccttcgca agatcgccga
     1921 tgtcgccgag atccttgaca tcaacgtggg aattgagccc ctgaatcgct tcgaaaccta
     1981 tttcctcaac acctgcgagg acgcggccgc cctgtgtgac gagatcggca gcacccatgt
     2041 cggcgtccac tacgacacct tccatgcgaa catcgaagaa aaggatccca cggccgccct
     2101 gcgcaagatt ggcaggaagc ggttgttcca cgtccatgct tgtgagaatg atcgcggcat
     2161 tcccgggacc ggccacgtgc cgtggaagga tttcttcaag accctcaagc aactcaagta
     2221 caaccgctgg ctgacgatcg agagtttcgt gccggcgatt aaggaactgg ccgcagcagc
     2281 ggcgatctgg agaccgctgg cgaaagacgg cgatgacctg gccaagcgcg gcctgaggtt
     2341 cctgcgagcg aatgtcgggt agggccgacg cgcgtgcggg tggtctcctt tgccgtgacg
     2401 gcagcgacag agccagggga ggcttcgttc cgtgagtggt caggcgagcc acgggaatca
     2461 gtccggcatg ggtgggacaa catggttcga cgtgacatga cgatcacgct ggtttccggt
     2521 ttgaccgcgt tggcggcggc gtcggctcgg ggtgggccaa acctgatccg caatcccggc
     2581 ttcgaggaag gcgaatcgcc cacatatccg ggtgtggggc tgcattggga aaccaacgac
     2641 gcccagccgc acccggacat tgatgtcctg acaacctcga cgaaatactc gggggtccat
     2701 tcgcaatggc tcaaggccca cccaacctgg gatctgggca tggtccgcca ggtgacgcca
     2761 tacggttcta tcagtcctgg catgatatat cgcgtcagcg cctggatcag gaccgccaac
     2821 gtgcagaacc ccgccggctg gtacgtattc ggcatatggt atctcagcaa tgacacctgg
     2881 atcggtgaat cgaagatgcc gcggcaggaa gcgctcaact acgattggcg cgagattacc
     2941 tggaccgtcg tcccgcctga aggaacgaat cgcatagccg tcttgttgac ccgccatacg
     3001 gacggggacg catggtatga cgacgtgtcc atcacccagg tatctgcggg accgccgcag
     3061 atatccgtct cgccgggttc gttcgagatc aaagccgtca agggacgacg catcgagagt
     3121 ggtacgctct gggtacagaa cgtgggcggc gggatcctgg agtacgccct caccgacgaa
     3181 gtcaggtggc tgagctgcgt gccctcggcc ggcacgagca caggcgaagt cgatcgcatc
     3241 atcattgcct atgacatcgc cggcttgtcc gttggcttct accgggcgag catcaccgtt
     3301 gctgatccgg cggcggtcaa tccggtggtt gccgtgcccg tggatttgac ggtctcgacc
     3361 ccgggcgatc tggattatga cggcgacgtc gaccagaccg actacggcct gttccaggca
     3421 tgttattcgg gaaacggcat cgaggcggcc gagacgtgca ccgacgcgac tatggacgcg
     3481 gacgctgacg tcgacttgga cgatttcagt ctcttctggc tatgcctcag tggaccgggc
     3541 attgaagccg acgagcactg tgcggactga gcttacccga cgggtcgccg gcagggtggt
     3601 tgagcgcaca caaataaaac ggccggccct caggccggcc gttttcgata aggctctccc
     3661 gtcagacaat catcgtcagg cttctttgcc ttggtctccg ggaagctcgc ggcgcgattc
     3721 agcagcaagc aactgctcgg actgatagtc gtaataactg cgaatctgct cgcggaaata
     3781 gcctcctcgg gttacttggg ccgcgttcag gattgccccg aaaatgtgtc cctcaacccg
     3841 ctcgatctgc tcgcgggcac gttgggcaac gccgcgagac gtgttttggg ccctgaacac
     3901 caggatgacg ccgtcgacgc ttggggtcat gaccagaacg tcgctgacca gcagcaccgg
     3961 aggcccatcg aaaatcacct gatcgtatcg ctcggtggcc tgggtaatca cttgctgcaa
     4021 ataggtcgcg gcgagcaact ccgtcgggtt gggcggaatg ggaccggtgg tcagcacatc
     4081 caggttgggc aattcggtct tggaaaccac atccgcgagg cgggtgcgtc ccagcaggac
     4141 gttgctcagg ccgtctatat tggacttggg aaaaatggtc cgaagcctgg ggcggtggaa
     4201 gttcgcgtcg accagcaaca cccgccggcc gctttgggcc agcgagatcg ccaggttgac
     4261 cgctacggcg gttctgccgt cctccggtct ggggcttgtg atcagcaccg tccgctgtcg
     4321 gtcggccgga caggtcaaca gcaggttcgt ccggatgttt cgaaacgcct ccgcgaccat
     4381 ggatcgaggc gccacatgcg gcgcaagctc gattgcgtca atggggattt cctcgtcgtc
     4441 caggtccggc acagtgccga gaaccggcac gtgaacatgt ctgatgacgt ctcggcccgt
     4501 cttcagggac gtgtcgatga actccagaag caccgccaag ccgacgccca acaacaggcc
     4561 aagtacgaaa ccggccggca ggttgtactc ccatcgaggc gaagatcgct ccaagggcac
     4621 caaggccctc acccccgaaa ggcgaatggc actggtggtg cggatcagca tgttcaactg
     4681 ggcaatgtaa tcgctcacgc gctcaagctg ctgctggaga agctcgtact ccttttcacg
     4741 gttttcatac tcgacgatct gccggtccag gtcacgctgc ttggatcgct cctgctcgat
     4801 ctgttccagc agttgctgtt cggcatgcat cgcgttgaaa tacatcgtct gcgcctgggc
     4861 gatttccgct tccagaaccg cctgctcctt tcgagccctg gcatcggcca actgattgtc
     4921 gatcgactcc atctgacttt tcagctcgcg gatctcgcga tggttttcgc cgagcccctg
     4981 ttgttgacgg gccaacagaa gctgctcctg catgaccctg gcgttttgta gctgcaccac
     5041 ctccggatcc tgctcgatca tcatccgcat ctgaggggta atgaacgcct gggacggatc
     5101 gcggttgacc agattctcat attgggcctg gtattgcagt ttctccgtac cgattcttgc
     5161 ggcctcctgg gccaagacgc gccaggtctc gcccacaata ttgattccct tgatcgcgcc
     5221 cggctcggtc aactccatct tcaggaaatc cgcctgttga tcgcgaaggt tctgcaacct
     5281 ggctctgatc gcttcttctt ctctagtgta ggtgtcaagt tcctctcgat agcttctctg
     5341 agagccggtc gtggtctcaa aaatgtactt atcaatgagc tggttgatga tgaccggcgc
     5401 gtcttccgga ttcctcgtcg acaggctgac cagaatgtag tttgtctgcg gtatttgttt
     5461 gacgctcaga ctgctctgaa gcttctgaac gcgttcgttg gcgtcgcggc ctgatttgaa
     5521 ccactgcgtt tcgcgaacgc gggcatcgtt ggtgatcagg tctctgagca acccttcgct
     5581 cttgagccga accatctggt cggccaccgc gcggtcgagc aggtccacct gcacgatcgg
     5641 gttcgtaaac tccatgggcg ctttcggcag cggcgactcc acaaccacct gccccattgc
     5701 ggtgtagcct ggccagtact ttacccacag gtaggtgccc ccgatagtca ggccgaaaat
     5761 gagaatccag atgaacagga taaggaaaat ccgctgcttc agaatgcgga tcacgtcctg
     5821 gaccgtgatg ctcgtccctg caagctcggg gcgaatgatc ggccccatcg gcatgggaat
     5881 ccgttcctga accgtcatgg gtaacgaagt catcccgcaa tacctcccgt ggccgttccg
     5941 tcctcaggct cgggttgatg gtccacaaac aaatgattca agacgtacga tgtcgcaccg
     6001 tagagcgaga aggcgacggc aagcatcatc agccccaaca gcgtatgagc cgtgccttgc
     6061 gccagatcct ggcgaccgaa aacgacgaaa aacccggtcg tggtaacccg gatgatgttg
     6121 cagaacacgg cgatcggcac gcaaaccaga atcatgatca gccgctgcca aagggcccgc
     6181 tcgttcacga aggtcatggc caccccgaga gccgtcatcg tcatgagcag acgcatgccg
     6241 ctgcacgccc gctccacgtc gagcgtcccc gtccggttct ggtagctgta ttcgacgatc
     6301 gtgcccgtcg cctcggtcat catgtccgga accatggaga gaacccccgc cgagacgcga
     6361 gcggcgattc gctggagcgg gctggtaatc tgtccgtata cgttgtctgg aagcggcatc
     6421 gcgaacataa ggaaggcgac ggcgaaccag ctccatcggg tcagcggcca gccgcatacc
     6481 atcaacagag tcccgacgat gctcatgacc aaggcgacgt tcttgggata gtcgatccgg
     6541 gcgatggttg ccttcagata cagcagaaac gcgagagtca ggaatgctgc ccctgccagc
     6601 cgcgggagta cgctgcggtc ccgcaaaccg accggcatca tgtccctgcg aatgtacagg
     6661 tagtacaggc tgaacaaagg aatgataaac ccgtgcgacc agtcctcgct ggacagccac
     6721 ttgagcacca gacgatgcag cacgtgccag aaaaggacgg ccagcagagt ggtcaacgcc
     6781 gctgcctgca gcgtgttggc cggcgtccac agggatcgag gcttggtgct catgtttgtt
     6841 cagcgccctt gcgcctcaga agtcgaaggc gctcagcaga cctctccgtg atgcctcggt
     6901 ccgggctcga tagtactggc cggcaagcgt gtcgtactcg gcccagttcc tgtcgtacga
     6961 aaacgagaat ccgtagctga accggaaggc attgcgaatg gtatacaaga agggggcaat
     7021 cgcgtgcgtc cccacgttga ccacgtcatt ggggcgcagg aagacgtccg ggtcctggcc
     7081 cgccatgatt cgagccaaat cccattgcgt gatctgctcc cgatcgcggt cgattctccg
     7141 gcggatctcg caccgcgtcg gccacgccag cggatcgaga ccgcgagcgg ctatcactgc
     7201 ttgtgccagg gtcacctgct caccgttgag cgggtattga cccggcatga ccacgtgacc
     7261 acccacgtag taagtcccga tcgggccggg atcaacccgg atcagatcct gatgtcgaat
     7321 cacaatgttg ctgctgctgt caccggttcg caaagcctcg gcggagatgc ggatgattct
     7381 ctgttgtccc tcgccggcca actcgctcca gtccaccggt cgggttgcct gctcagtctg
     7441 tggaaccgtc tccacgacgg tcgaagccga agtcgccgcc cgacgggctt cctggcccgc
     7501 aggcgcctca atcagcttgt cgttgacgaa gatgaaaggt ctcaacgggg gcgggctctc
     7561 gttcttcgat tgcggcgcgg aggaaggcaa ctcaccccgt cgaggagatg ctccctcggg
     7621 ggcgacggcg tcgatcaaat cctgctccac ggagtcggtg ggcaatgccg gcccctgcgt
     7681 tcctggtcgt gtggacggct ggaccgactg aggctcggga acagccggct caggaacgac
     7741 ggccgacggc aacccggtgt ctgccggctt aaggttggtt tcatcacggc ttggcttgcc
     7801 cttcacgacc gattccttga ccgtcttcgg gcgccgctcg ttccggatca cgtagatcgt
     7861 ccggacccgg ccgccggcgt aaagccgctg aagcatctca tccgacccgc cggccatgat
     7921 gagtgcttcg gtgagcctga aatccgggcg gggaatccga taggtgccgg gcgcctgaac
     7981 ggcgcccgta atactgaaag tccggttctg ctgatcgccc attatcaccg tgattgtggg
     8041 ttcgctctct tcggcataga tgcctcttgc cttcgcctgt cgagcaacct cgtctctaac
     8101 ctcaggaaga gtaagcccct ccacgcgaat tcgaccgatc tgtgcgatgt caacatagcc
     8161 caggtcgtcc acggtcagga cgaattcgct ctgtgcccca cgggccatga aatccagaag
     8221 cgtgatctga aaaacgtcgc cgggacccag ctggtagttc tccaccgtgg caaccaagtc
     8281 gtccggcgtc gggtccaccg cgttgggaat accggaaggc ttgtcctcga aactgaccgt
     8341 gtcctggatc tccgtgacca cgttctcgcg gaagttccca acctgagtag ggtccaggaa
     8401 cccattccac catgccgagg cacaacctga gatgaaggcc accggcaaga gagtgcctgc
     8461 caatccaaac ccccagcgaa ccgggctgta tttcgggttt aatcgcatat gagtccgtcc
     8521 tgactctgag ctggataacc tacctgccga aacaaggcaa ggctctcgag cccccgctcc
     8581 tcgggatcac ctattcacct gggcaaaaac gcccagcgcc ccagccaccc tgtggggtgg
     8641 cgcgaaaact gcgctgtcat tccgtctgcc tttgtgtttt ttcgcagact gatccgtaag
     8701 ttatcggcac acaggcagtt ggcggttgag gatgagccgc caaagatggc ccagttaagc
     8761 accctcccgg aaatccactc acagctactg taccgctttg ccggccagaa ggttcgcgat
     8821 ttcaagtcat ttgtgctgct tcaatcctgc caaccggcta cacaatactt tggggcagga
     8881 agagcgacaa gacggctaag cgtccacggc tcgta
//
LOCUS       DAOTPW010000239         8888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7167_length_8888_cov_5.418657, whole genome shotgun sequence.
ACCESSION   DAOTPW010000239 DAOTPW010000000
VERSION     DAOTPW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7167_length_8888_cov_5.418657"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="P5316_17745"
     CDS             <1..773
                     /locus_tag="P5316_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86855.1"
                     /translation="NRLPPGNYLFFAAAPLIPDVTLGDVVPAPVLLDWDDTHPVLRHV
                     NVATMSLFSCHKPELPSDAQTLIEATSGPVLSLLTRERRQYLICSFSLFDEDRTHVNT
                     DWPYQEGLVMFMFNALRFLSGSSTIGQQPSVSPGQAFTVSVKPGTREITVRRPDGTTD
                     KAAASASGLATYGRTNRVGLYHLETGIPGEDARAVNLLDASESLIASNKALRVASGET
                     VAVRGSDYVNRPLWPWILGGLGAILLVEWIVYNKRVFV"
     gene            complement(844..2145)
                     /locus_tag="P5316_17750"
     CDS             complement(844..2145)
                     /locus_tag="P5316_17750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRR86856.1"
                     /translation="MVVLVGAGLLQHFQLGVDEQEAEKVRAVAMAETYATQAAAVMRS
                     GRGAELEAFVDKTPLHPSACLLAVLDRHDHALAARGNKALIRRFVELGFKGPQSLEPG
                     AWSLAGDPEHKVPPLTLVGVPIVQPDGAGQLGFLVYAARLSGQDGLATGRIARFFLGL
                     VIVSLIGLAAGFSWMRSRVLQPLAELVSQVKARESSGEEEMPATLTDRGDEMGVIARA
                     IVRERQELKGWQSRALQIERVADTRLAAERQRISRELRQAEKKITTDPLTNLGNRQLL
                     MDRFVEIFDTQKRAGHDLSVILIDVDHFKEVNDTLGHKAGDELLVFVGELLRQCLREH
                     DMAVRYGGDEFLLVLPSVTLSNAAAVAERTIRLFAQQAGVLAVQPKPSMSAGVASLVA
                     HMPASAEELLEMADQALYEAKKAGKSLVRVAGGVAVATEPS"
     gene            complement(2322..4388)
                     /locus_tag="P5316_17755"
     CDS             complement(2322..4388)
                     /locus_tag="P5316_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86857.1"
                     /translation="MRDIADKFVGGIQRIDSALPVGVRGPTWRSVLIGFTGVAALSLL
                     EPYNQNTFKNTPLIGNQFPVAVVFFIVVLVLVVNPILSLLSSRFSPSHVGPARRVFAI
                     VRPLSPSELVVIVTMVLSACAVIWSGFHRFWAHQLVAPFYYLGSHPRWEPLVRSLPSW
                     LVPSADPENCEIIRSFFAGGAEVPWGPWLRTSLLWSPFVLAFFVGCFFMVAFFRRQWA
                     EAEKLSFPLAAITLEMLRPPASGRLVNDLFRSKLFWWSLAVVVFFQMLYGLHACFPAF
                     PEVVFKYDLNPAFDAPPWRYLPPWIKAKDAFFMMVGVSFFLSTEMSFSLWVSVIILAV
                     IEMLGKTYQYPIHEQFNDHQIGAYIAYTVVILYGARRHVARIVRSIWSGPGNGSDGVE
                     LSERAAAIGFVVCFVVACAWLRLAGLPLSLAILVAAVVFMLALVVGRVVAESGLLFVQ
                     YGCWPTRIAESCFGSSLTPATHTLIHFTTVAPTIDARESLAPFAFNSARVADGVKHLH
                     RNRLFLLWIGALVLALVLAGYAQLSIVYRRGALQTDEYAGRFAPEILYNSSADFDSLL
                     QQPPERVRADLTRHLRQVACGGGVVLALCFLRYRFLGWPLHPVGFLLAHSYPMRVFWL
                     SIMLGWLCKTLVMRLGGVPVYRRLSPVFMGMIVGTAFSSVFWIAVRMFICGMGEQGRP
                     ISFLPS"
     gene            complement(4402..6348)
                     /locus_tag="P5316_17760"
     CDS             complement(4402..6348)
                     /locus_tag="P5316_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRR86858.1"
                     /translation="MREDRELAEFRGIMPPPDSWESGFNLKSVFAALFIGTVMMPGSM
                     YMGLIAGAGLGPAAQWVTVILFMEIARRAFSTMKSPEVFVLYYMAGAAITLPYTGLLW
                     NQFLVQSDAAEATGLTRQFPTWFAPTEDVLKQRTFFHWGWAPAITLVALQQLFTRIER
                     FGLGYILFRITSDVEKLPFPMAPVGAAGIVALAETSTGQRSWRWRVFSIGSVIGLAFG
                     FVYVGIPAVSGTLLLEPIQPLEIPWTDLTWRTETVLPAVPTGISFDLGHYILGMVLPL
                     WAVVGEFIGMLLTMIANPILHRAGVLTWSPGSGTIGTQFSNSLDFYFSFGIGVTFAIA
                     LIGFYHLFRSMRHRDGDSSRPDWRRLFRPPEGRGDISLWVAVAIWVGSTLTYISLCRF
                     WLIPDFPLWILLAYGFLYTPIVSYVSARMEGVVGQYVDIPLVREAAFILSGYQKIDVW
                     FAPIPLTNDSGNVVYFRQVELTGTRIRSVLKAELVILPIVLVAGVLFSQFIWRLAPIP
                     SPVYPFTQKMWELQAYNMCLTYSATTEGGGLFARAFQPTYVLAGTGISVAAYAFMARF
                     GLPVMLAYGLIRGMGGGMLPHFVIPHFVGAMIGHYFFRRRFGLQWRQYAPVLMAGFGC
                     GMGLIGMLALGFVLVSKSVFPLPY"
     gene            complement(6456..8756)
                     /locus_tag="P5316_17765"
     CDS             complement(6456..8756)
                     /locus_tag="P5316_17765"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86859.1"
                     /translation="MTKETTAPTARAGTALLAAVFLLSACGLMFEITLTRFFSATIWY
                     HFTFVAISVALFGWGLGGLFIFLLRLSRFEQHVRSILVGLSLLLAVTLPLFLAGVLQL
                     PFSPDRLNTYFLLSLMPFLAGGAVLSLMFESWGKDSNRLYFADLIGASLGTLLVPLAI
                     GRLGAESAILVIAVLPSLAAVLLSLSLPAPSKAKWLPASVAVLAACIGLTVWNVRTGT
                     MTVRDAPEKELYKLLAKHPGQARIDFDKWNAFSRITSVEGFSEDYVRRIFIDSSAETS
                     VMHWDGTPNTPPDARNWFRAFPFRMMKNPEVLVIGPGGGTDVVLSIAAGASHVTAVEM
                     NDLIVECVRGLGAQAGNLYDHPKVELVMDEGRNYIQRCGRKFDMIVLGWVDSWASVAG
                     GGLALTENYLYTREALEAYYDRLSDNGALVIIRWPVDVPRLVANTVSFMSNRGMNMEE
                     ISRHIVAVSMRKPGVGEESAVETVFMMSRSPLTEQQVDALLAGHDDAHLWHAPFRQCD
                     PPYSDLFSGNISFAQYTDAFPILATPVTDDHPFYFAWDKPRGIPDFVKRLLQVPMAGV
                     VGFTLLLLIATRYAGLRAPGPRTVAYFGALGVGFIVVEVALIQRLILLLGHPIYTLVV
                     ILFTLLLAGGCGSFFARRFAPQGIRSALGKIIPLVVLLVILAAFVLPVLVDKALPLSL
                     PLRIMITVLMVIPYGFLMGMPFPLGLRKQSQDPAGSPASVLWGINGVASVIGSIGGVA
                     LAVAVGFTWVFLAGAVCYTVAWATRP"
BASE COUNT         2056 a         2970 c         2514 g         1348 t
ORIGIN      
        1 ccaaccggtt gccgccgggt aactacctgt tcttcgccgc cgctccgctg attcccgatg
       61 tgacgctcgg cgacgtcgta cccgccccgg tcttgctcga ttgggacgac acgcaccctg
      121 tcctccggca cgtcaacgtc gccaccatgt cgttgttctc ctgccacaag ccggaactgc
      181 cctccgatgc tcaaacactc atcgaggcta ccagcggccc cgtcctgtct ctgctgaccc
      241 gcgagcgccg ccagtatctg atctgctcgt tcagcctttt cgatgaagac cgcacgcacg
      301 tgaataccga ctggccctat caggaagggc tggtaatgtt catgttcaat gccctgcggt
      361 ttctctcggg aagttcgacc atcggacagc agccgtcggt cagccccggg caggcgttca
      421 ccgtcagcgt caagcccggg acccgggaaa tcaccgtccg tcgcccggac ggcaccaccg
      481 acaaggccgc cgcctccgcc tcgggtctgg ccacctatgg aaggacaaac cgcgtcggtc
      541 tctatcatct cgaaacggga attcccggcg aggatgcgcg ggccgttaat ctcctcgacg
      601 cttcggaaag cctcatcgcc tcaaacaagg ccctccgagt cgcctcgggc gagaccgtgg
      661 ccgtccgagg aagcgactac gtgaatcgcc cgctatggcc ctggatcctc ggaggcttgg
      721 gcgctatcct tctggtggaa tggatcgtct acaacaagcg ggtcttcgtc tgagaaccca
      781 actgaacggc ctgcctgcgc gatgatccgt cgcggcacag caaccggcgt gcaggccgct
      841 gcatcaagac ggttcggtcg caaccgcgac accgccggcc acacgaacca gcgacttgcc
      901 tgccttcttg gcctcataca gagcctgatc ggccatctcc agaagctcct cggccgaagc
      961 aggcatgtgc gctaccagcg acgccacccc cgcgctcatc gaaggcttgg gttgcacagc
     1021 cagcactccg gcctgctggg caaacaaacg aatcgtccgc tccgctaccg ccgccgcgtt
     1081 tgaaagagtg accgacggca gcacaagcag gaattcgtcg ccgccgtacc gaaccgccat
     1141 atcgtgctcg cgaagacact gtctgagcag ctcgcccaca aaaacgagca actcgtcgcc
     1201 ggccttgtgc cccagcgtat cgtttacctc tttgaaatga tcgacgtcta tcagaataac
     1261 cgacaaatca tggccggccc gtttctgagt atcgaagatc tcgacaaacc tatccatcag
     1321 caactgccgg ttgccgagat tggtcaacgg atcagtggtt atcttcttct ccgcttgccg
     1381 cagctcacgg ctgatgcgtt gtctctccgc cgcaagacga gtgtccgcta cccgctcgat
     1441 ctgcaaggcc cgcgattgcc accctttcag ttcctgtcgc tcccggacga tcgcccttgc
     1501 gatgacaccc atctcatctc cacggtcggt cagcgtcgcg ggcatctcct cttcacccga
     1561 agactccctc gccttcacct gactgaccaa ctcggccaac ggctgcagca ccctggaacg
     1621 catccacgag aaaccggccg cgagcccgat caatgagaca atgaccagcc cgaggaagaa
     1681 tctcgcaatt cttccggttg ccaaaccatc ctgccccgaa agccgggccg cataaacgag
     1741 gaaaccaagc tgcccggcgc cgtcgggctg cacaatcggt actcccacca gggtcagagg
     1801 cggtaccttg tgctccgggt caccggcaag actccaggca ccaggttcca atgactgtgg
     1861 acctttaaac cccagttcca caaaacgcct gatcaacgct ttgttgccgc gggctgcgag
     1921 agcatgatca tgccggtcaa ggacggccaa caggcaagcg ctgggatgca gcggcgtctt
     1981 gtccacaaaa gcctcgagct ccgccccgcg accgcttctc atgaccgcgg ccgcctgcgt
     2041 ggcgtaggtc tcagccattg ccacggctcg caccttctca gcttcctgtt cgtcgactcc
     2101 gagctggaaa tgctgcaaaa gcccggcacc aacaagtact accatcaccg ccaggctcac
     2161 gccgaaccat aagctctgtc ttacgcacga gttgcgctct gctgccatcg aaacgtccct
     2221 gcaccgccac ccacctggcc gctgcacgca aacggatgca gttcatatcg gttaatcaac
     2281 ccagcatgta aagcaggtcc tcgctgaata tcggacgtcg ttcaactcgg aaggaaactg
     2341 attggccggc cctgctcgcc catgccgcaa atgaacatcc tgacggctat ccagaacacg
     2401 gaactgaaag ccgtgccgac aatcatgccc atgaaaaccg gactcagccg gcggtacacc
     2461 gggacgcccc ccagccgcat gaccagcgtc ttgcacagcc agcccagcat gatcgaaagc
     2521 cagaatacgc gcatcgggta gctgtgggcc agcaggaatc cgaccggatg aagcggccaa
     2581 cccaggaagc ggtatcgcag gaaacagaga gccagcacta ccccgccccc gcacgccacc
     2641 tgacgaagat gacgggtcag atcggcccga actcgctccg gcggctgctg caaaagactg
     2701 tcaaaatcgg ccgagctgtt gtagaggatc tccggcgcga atcgaccggc gtactcgtcg
     2761 gtttgcaggg cgccacgacg atacacaatg ctcaactggg cgtagccggc cagcaccagt
     2821 gccagaacca acgcaccgat ccacagcagg aagagccggt tgcgatgaag gtgcttgacc
     2881 ccgtccgcca cacgggctga attaaaagca aaaggagcca aagactcacg ggcatcgatt
     2941 gtgggggcga cagtcgtgaa gtgtatcaac gtgtgcgtcg ccggagtcag cgacgacccg
     3001 aagcacgact cggcgatccg cgtcggccag cacccatatt gcacaaagag cagccctgac
     3061 tcggccacca cgcggccgac aaccaacgcc agcatgaaaa cgaccgccgc caccagaatc
     3121 gccaggctta acggcaaacc cgccagcctg agccacgcgc acgcaaccac aaaacaaacc
     3181 acaaagccta tggcagccgc tcgctccgac aactcgacac cgtcagaccc attccccgga
     3241 ccgctccaaa tggaccgtac gatacgggcc acatgccggc gggcaccgta gagaatgacg
     3301 accgtgtagg cgatatacgc gccgatctga tgatcgttga attgctcgtg aatgggatac
     3361 tgataggtct tgcccaacat ctcgatcacc gcgaggatga tcaccgacac ccacaggctg
     3421 aacgacattt cggtcgaaag gaaaaacgag accccgacca tcatgaagaa cgcgtccttg
     3481 gccttgatcc acggcggaag ataacgccag ggcggggcgt caaaggccgg gttcagatcg
     3541 tacttgaaca ccacctcggg aaacgccggg aaacacgcat gcaggccgta aagcatctgg
     3601 aagaacacca cgacggccag cgaccaccag aacagcttgc ttctgaacag atcattgacc
     3661 aggcgacccg acgccggcgg ccggagcatc tccagggtga tcgcggccag cggaaatgac
     3721 agcttctccg cctccgccca ctgccgcctg aaaaaagcaa ccatgaagaa gcagccgaca
     3781 aaaaacgcca gcacgaacgg cgaccacagc agggacgtcc tcagccatgg cccccagggc
     3841 acctccgctc cgccggcgaa gaagcttcgg atgatttcgc agttctccgg atcggccgac
     3901 ggcaccagcc acgacggcag cgatcgcacc agcggctccc accgcggatg agaccccagg
     3961 taatagaacg gagccacgag ctggtgagcc cagaatcggt gaaaaccgct ccagatgacc
     4021 gcacacgccg agagcaccat cgtcacaatc acaaccaact cggacggcga caacgggcga
     4081 acaatggcga agacgcgacg cgcgggcccg acgtgcgatg gtgagaaacg cgacgacagc
     4141 aggctcagaa tcgggttgac gaccaacacc aggacgacga tgaagaagac caccgcgacc
     4201 ggaaactggt tgccgatcaa gggggtgttc ttgaacgtgt tttgattgta cggttccagc
     4261 aggctgagcg ccgccacgcc ggtaaagccg atcaataccg atcgccaggt cgggccgcgc
     4321 accccgacag gcaaggcgct gtcgatccgc tggatcccac ccacgaactt gtcggcgatg
     4381 tctctcatcg ctgcctgcct gtcaatacgg caacgggaac acagatttcg aaaccagcac
     4441 gaagcccaaa gccagcatgc cgatcaggcc catgccgcaa ccaaatccgg ccatcaagac
     4501 cggcgcgtat tgccgccact gcaacccgaa ccgccgccgg aagaagtaat gcccgatcat
     4561 cgctccgacg aagtgcggga tcacaaagtg cggcagcatg ccgccaccca ttcctcgaat
     4621 caggccatag gcgagcatca cgggcaggcc gaaccgggcc atgaacgcgt acgccgccac
     4681 cgaaatgccc gtgcccgcca ggacataggt cggctgaaac gcccgggcaa acaacccgcc
     4741 gccctcggtc gtggccgagt aggtaagaca catgttgtag gcctggagct cccacatctt
     4801 ctgtgtgaac gggtagaccg gcgagggaat cggggccagg cgccagatga actggctgaa
     4861 cagcaccccg gcaaccagca caatcggcaa aatgaccagc tccgctttca gcacgctcct
     4921 gattcgcgtc ccggtcagct ccacctgccg gaaatagact acgttgccgc tgtcattcgt
     4981 cagcggaatc ggcgcaaacc agacgtcgat cttctggtaa cccgaaagaa taaacgccgc
     5041 ctcgcgaacc agagggatat ccacgtactg cccgaccacc ccttccatcc tcgcggagac
     5101 atatgacaca atgggcgtgt acaggaaacc gtaagccagc aggatccata gcggaaaatc
     5161 cgggatcagc cagaaacggc acaggctgat gtaggtcaat gtcgagccaa cccaaatggc
     5221 cacggccacc cagaggctga tgtcgccgcg accctcaggg gggcgaaaca ggcgacgcca
     5281 gtcggggcgg gaggagtcgc catctcgatg gcgcatggac ctgaagagat gatagaagcc
     5341 gatcagggca atcgcgaagg tgacgccgat cccgaaactg aaatagaagt ccagcgagtt
     5401 ggaaaactgc gtgccgatcg tgccgctgcc gggcgaccag gtgagcacgc cggcccggtg
     5461 cagaatcggg ttggcgatca tcgtcagcaa catgccgatg aactcgccca ccaccgccca
     5521 caggggcaaa accatgccca gaatgtaatg cccgagatca aacgagatac ccgtcggcac
     5581 ggccggcagt acggtctccg tccgccacgt caggtccgtc cagggaatct ccaacggctg
     5641 gatcggctcg agcagcaagg tgccgctgac tgcgggaata cccacgtaga caaagccgaa
     5701 cgccagaccg atcaccgagc cgatcgaaaa cactcgccaa cgccacgagc gttgccccgt
     5761 cgacgtctca gccagagcca cgataccggc cgcacccacc ggggccatcg gaaacggcag
     5821 cttctccaca tcagacgtga tccgaaaaag gatgtaaccc aaaccgaatc gctcgatccg
     5881 cgtgaagagc tgctggagcg ccacgagcgt gatggccggg gcccagcccc agtggaagaa
     5941 cgtccgctgc ttgagaacgt cctcggtcgg ggcaaaccag gtaggaaact gacgcgtcag
     6001 cccggtggcc tcggctgcgt cagactgcac caggaactgg ttccagagca agcccgtgta
     6061 cggcagcgtg atcgccgctc cggccatgta gtaaaggaca aatacctccg ggctcttcat
     6121 cgtcgagaac gcccggcggg caatctccat gaagagaatc accgtgaccc actgagccgc
     6181 cggcccgagc cccgccccgg caatcaggcc catgtacatg ctgcccggca tcatgaccgt
     6241 gccgatgaac agggccgcga aaacactctt gagattgaag ccgctctccc aggaatcggg
     6301 agggggcatg atgcctcgaa actcggctag ttcgcgatct tctctcatga gctgccgaga
     6361 gtgtaagcgc ggcagctttg cgggcaaagc ggttgaggac caggtgagcg ccagcggcct
     6421 ccttgtcagc agcacgccgc cagtgtctca aacacctacg gccttgttgc ccacgccacc
     6481 gtgtagcata ccgctccggc gaggaacacc caggtgaagc cgacggccac cgccagggcc
     6541 acgcctccga tcgagccgat caccgaggca acgccgttga ttccccagag caccgatgcc
     6601 ggcgagccgg ccgggtcctg cgactgcttg cgcagcccca gcgggaaggg catacccatc
     6661 aagaacccat agggaataac catcagaact gtgatcatga tccgcagcgg caggctcagg
     6721 ggcagagctt tgtcaaccag tacgggcaaa acgaacgcag ccagaatcac cagcagaaca
     6781 accagaggga taatctttcc caacgccgac cgaataccct gtggcgcgaa ccgccgggca
     6841 aagaagcttc cacacccacc cgccagcaac aaggtgaaca ggatcaccac cagggtgtaa
     6901 atcggatgcc caagcagcag aatgagccgc tgtatcagtg ccacctcgac gacaatgaaa
     6961 ccgacgccca aggcgccgaa gtatgctacc gtcctcggcc ccggcgcccg caggcccgcg
     7021 tagcgcgtgg cgatcagcag cagcaatgtg aaacctacca cacccgccat cggcacctgc
     7081 agcagtcgct tcacgaaatc gggaataccc ctgggtttat cccacgcgaa gtagaacgga
     7141 tgatcgtcgg tgacgggcgt ggcgagtatt ggaaaggcat ccgtgtattg ggcaaagctg
     7201 atgttgcccg agaacagatc ggaataaggc gggtcgcatt ggcggaaggg cgcgtgccac
     7261 agatgggcat cgtcgtgacc ggccaggagg gcgtccacct gctgctcggt cagcggtgaa
     7321 cggctcatca tgaagaccgt ctcgacggcc gactcctcac ccacaccggg cttgcgcatg
     7381 ctcaccgcca cgatgtgacg ggagatttcc tccatgttca tgccacgatt gctcatgaac
     7441 gacacggtat tggccacaag tcgcggcacg tcgacgggcc agcgaatgat caccaacgcg
     7501 ccgttgtcgc tcaggcggtc gtagtaggcc tccaatgcct cgcgggtgta gaggtagttc
     7561 tcggtcagtg ccaatccgcc gccggccacc gacgcccacg agtcaaccca gccgagcacg
     7621 atcatgtcga acttgcggcc acagcgctgg atgtagttcc gcccctcgtc catgaccagt
     7681 tcgaccttcg gatggtcgta gagattgccc gcttgggcac ccaggccgcg cacgcactcg
     7741 acgatcaggt cattcatctc caccgctgtg acgtgcgagg ccccggccgc aatcgagagg
     7801 acaacatcgg taccgccgcc cggcccgatc accagcacct ccgggttctt catcatccgg
     7861 aaaggaaatg cccgaaacca gttgcgcgca tccggcgggg tgttcggcgt gccgtcccag
     7921 tgcatgacgc tggtctcggc gctgctgtcg atgaagattc ggcgaacata atcctcgctg
     7981 aaaccctcga ccgacgtgat ccgcgagaaa gcgttccact tgtcaaaatc gatcctcgcc
     8041 tgacccggat gcttggccag gagcttgtac agctccttct ccggcgcatc ccgaacggtc
     8101 atcgtgcccg ttcggacatt ccacaccgtc aacccgatgc aggccgccag cacggccacc
     8161 gaggcgggca gccacttggc cttcgaaggt gcaggcaacg acaggctcag caagacggcc
     8221 gccagtgaag gaagcacggc gatcaccaga atggccgact cggcccccag ccggccgatg
     8281 gccaggggta ccagcagtgt ccccagcgac gcgccgatca ggtcggcgaa atacaggcgg
     8341 ttggagtcct tgccccagct ctcgaacatc aagctgagca ccgctccgcc ggccaggaac
     8401 ggcatcaggc tcaacaggaa gtacgtgttg agccggtccg ggctgaacgg cagttgcagc
     8461 acccccgcca gaaacagcgg tagcgtcacc gccaacagca gcgacagtcc caccaagatc
     8521 gaacgaacgt gctgttcgaa ccgcgaaagc cgcaggagaa aaatgaacag cccgcccaag
     8581 ccccacccga acagggccac cgagatggcc acaaaggtga agtgatacca gatcgtggcg
     8641 gagaagaatc gcgtcagcgt gatctcgaac atcagcccgc aggccgagag gagaaacacg
     8701 gcggccagca gggcagtccc ggcccgcgcc gtcggagcag tcgtctcttt ggtcatagtc
     8761 gaacccaaca aagccatgcc ggcgcggcgg ccggagtgtc agagggggaa attgtatcga
     8821 agtgccgcgc acacgcaacg cgctgacagg cgggaagccg tcatgggccc gccgtcatga
     8881 attgaacg
//
LOCUS       DAOTPW010000240         8849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7191_length_8849_cov_5.462133, whole genome shotgun sequence.
ACCESSION   DAOTPW010000240 DAOTPW010000000
VERSION     DAOTPW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7191_length_8849_cov_5.462133"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="P5316_17770"
     CDS             <1..905
                     /locus_tag="P5316_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86860.1"
                     /translation="FFTSTPVESRIPQGPRHAKGIDAENMIAADKPPVGAEIVTDDDL
                     PEQMSFGKGLKISGQAGTTVPITIPAPDDGRFSIEARLARGVKASDFEILQDGKPIGE
                     WVRLVKGPNTLSVRLSGKPVEGDRCCVILDFFILEPYRNFVRNWYLMGPFPNPSKMGT
                     DQTYPPETEPFKADQSFSGKRGPVGWRKIRVPSGLMVSDDKYFEDGESFVFYVYTEVI
                     SPDDRQATVYAGSDDGIKVWINGDLVHTCKADRALQPDQDRFDIRLRKGRNTVLIKLI
                     QNQGQWGLSFRIDDPADELQYVVP"
     gene            complement(1198..2964)
                     /locus_tag="P5316_17775"
     CDS             complement(1198..2964)
                     /locus_tag="P5316_17775"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HRR86861.1"
                     /translation="MTDQPASASIAPRVKPNRELVEPHEWIRRRIDDWHWRIRSDWLA
                     LFESSQCPSWLDLQNDPRARLFKSNEIRDVFRVQTDCGAVFAKIARPVTWLDRLRRLI
                     LGPDSLREWRAAEHAIRCGIATVVPVAAAWRSTNGREPASIFITMETPDAEQLDTWWL
                     RAGIGSSLRRNDRNAVIDAVAELIARAHHGAFVHTDLHAGNILVFPYGDGRYGACFVD
                     LQAVSIRRRLSNRRAIRNLAAFGNWFRFNASLTDRMRFLHAYMAWRGRLRPDLNLIQD
                     RRRLVGELEHAARIHANAQAAKRDRQALRNGRRFGILRLDRRTRARVFLWTKQTTPGS
                     AVPKTPLTRRWWRGQLASRVLSTPGTDRICRRSRSEHARGSFDLELEDGKSIPVVLLG
                     RRPTLGRQLKHVLFSSPEMRLWKTANALLNRGIPAARPLAVCERRCCGLLRYALVLIE
                     KPPGARSLHEFIQEQTRGLPPQERHRVRAGIAGQIGALFRKLDEEGFVYDKLKASDLT
                     ICLSRFSADKPVVVLSGYLDLRQARSRDPLANLPALIRLSADLRECPHVSRTDRLRVL
                     KRYLDRFGKPAADWRQVWREMG"
     gene            complement(2984..4285)
                     /locus_tag="P5316_17780"
     CDS             complement(2984..4285)
                     /locus_tag="P5316_17780"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRR86862.1"
                     /translation="MAAQTLLIKGGRIIDPASGLDKIADLLLADGKVAGMNHNLNAPG
                     AQTVDATGLIVCPGLIDVHVHLREPGDEDEETIETGTAAAVAGGFTSVACMPNTKPTL
                     DDEASIEFIYHQSQRVGSCNVYPVGAITKDRQGKELAEMGQMVRSGAVAFSDDGSGVA
                     NTQLMIQALNYVRMFDKPILQHCEDPDVVGGGVMNGGATAVRLGLPGISPIAEELMLQ
                     RDLTLIRATGARYHVCHVSTARAVELVRQAKAAGLPVTAEVCAHHLVLTEDQCATYDP
                     NFKMKPPLRTMEDVEACRRGVADGTIDCLATDHAPHGIQEKELEFLTAPFGIIGLESA
                     LPLFIRALVETGLMDWPAMIDRLTVRPAKMLSISKGTLAVGADADVTLIDPHCRWMID
                     VNQFRSKSRNCPFHGWEVTGRAVMTIVGGEIKHRLNRMAVA"
     gene            4469..6433
                     /locus_tag="P5316_17785"
     CDS             4469..6433
                     /locus_tag="P5316_17785"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRR86863.1"
                     /translation="MSRLVWAFLAAWVAMGLTMTEAVLSGEPTTQPAPVPASGQTLRD
                     LRAYLLRRAPKFVAPSDPGEWLPQAEQIRENVLRNVIFRGVPQQWQEGPVRIVWQETL
                     PGRGYKIRKLRYEAVPGLWIGALLYEPEGLTGKVPAVLNPNGHVGKPGMTIDYKQARC
                     INLAKRGMLALNYEWIGMGQLDGPGYAHGNASHLDLCGRAGLSVFYQSLKRGLDVLLS
                     HPSADPERTAVTGLSGGGWQTIVFSSLDTRVKLCAPNAGYCGIERRIWETRDIGDREQ
                     NPPDLVAIADYPVLTALLAPRPALLIYNANDDCCFYAATARLSVFEPVAPVYEMLGVG
                     DHFAFHVNSDPGTHNYLKDNRQAFYRFINRHFLPPDQRKDEEIPCEDEIRKPEELKIE
                     YPPDHADFYSLAAEAMRDLPATKCPAGAEIARKQWSDKTRDALAKVVRADLTLRTSPV
                     VTKLSVSTMPVGQTPGTAVHLRLGGQWTLPMVEYSPTKGSPTVCHLIVADGGIGKTRE
                     VAATALGRGERVIVADILLTGECVPGITGTWQFAQMIDAVGARPLGIQAGQLDAIIEH
                     LAWRYPGEVLHVTTIGRVSGLAALTTAALTPGRPARLTLQDMIPSLKDLITGKVSWNS
                     APSLFCFGLLEVADVPELIELAKPTQVSMQ"
     gene            6494..7450
                     /locus_tag="P5316_17790"
     CDS             6494..7450
                     /locus_tag="P5316_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010778674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRR86864.1"
                     /translation="MRIVVLDGHTMSPGDNPWDEVAALGELTIYERSTHGQLIERAGA
                     AEIIVTNKVPVTAADLDQLVNLKMIAVTATGYNVVDVEAARARGIPVCNVPVYATQGV
                     AQFTFALLLELCHHVGAHDASVKQGAWTRSPDFSYCETPQIELADRVMGVIGYGRIGR
                     QAARIARAFDMKVMACDKDHAPGGADGEVCFVDMRTVFSQADVVSLHCPQTSETAGLV
                     NAERLGLMKSTAFLINTARGGLVVERDLADALNKGRIAGAAVDVVAVEPIREDNPLLT
                     ARNIIITPHMAWAGLAARKRLMAVTAENIRAFQRGKPVNVVN"
     gene            complement(7456..8427)
                     /locus_tag="P5316_17795"
     CDS             complement(7456..8427)
                     /locus_tag="P5316_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR86865.1"
                     /translation="MLQSTLDRVSRKAAGTLTGFTLIEVLVVVAIIALLVAVLLPTLA
                     RTREQTRATVCLSNLKQQGIALSTYSADHKTVLPWAGSFRFSLMEGKYYLGYSGDDRH
                     DWALVNIGALYPKYVGANPELFYCPNNKAADSTGSNGIAQFLSCYRAPKRGRPGYQNS
                     HTFPISPFSSYNYAAPLIPAASPCDAGSRMYPEESVRYGDASLANESPYWQYLNEAVD
                     SAPEFLGPLPAGMRGRHSVHAFVSDGYFAGKDAFTWEGQVYNRDPYEGYHLRSYNVLY
                     SDLHARRVVDPQGQIHAAQLTPIRYNNAGGPNVTKVFKVWDYFSRNH"
     gene            complement(8494..>8849)
                     /locus_tag="P5316_17800"
     CDS             complement(8494..>8849)
                     /locus_tag="P5316_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRR86866.1"
                     /translation="STRITSSSTRPAWRSTLLGMIQIRRQGNDILLPVKVVPKSSRDR
                     IVGELDGALKITVAAAPDKGAANVAVCTLLAKTMGLRTSQVMVQTGHGSPRKTVRISG
                     TSIDAVLERLEIAKT"
BASE COUNT         1599 a         2593 c         2939 g         1718 t
ORIGIN      
        1 acttcttcac ctcgacgccc gtcgagtcgc gcatcccaca ggggccgcgt cacgccaagg
       61 gcatcgatgc cgaaaacatg attgccgccg acaagccgcc ggtcggcgcg gagatcgtta
      121 ccgacgacga cttacccgaa cagatgtcct ttggcaaagg cctgaaaatc agcggccaag
      181 ccggaaccac cgtgccgatc accattcccg cgccggacga cgggcggttc tcgatcgaag
      241 cccggctagc gaggggcgtg aaggcctccg actttgaaat cctccaagac ggcaagccga
      301 tcggcgaatg ggttcgtttg gtcaaggggc ctaacaccct cagtgttcgc ctgagcggca
      361 agccggtaga aggtgatcgc tgctgcgtca tcctggattt ttttatcctc gagccttacc
      421 gcaatttcgt tcgcaactgg tacctcatgg gaccgtttcc gaaccccagc aagatgggta
      481 ccgaccagac ctatccgccg gagacggagc cgttcaaggc ggaccagagc ttttccggca
      541 aacgcggccc cgttggctgg agaaagatcc gcgttcccag cgggctgatg gtgtcagacg
      601 acaagtattt cgaggacggc gagtcgttcg ttttctatgt ttacaccgaa gtgatctccc
      661 cggacgaccg acaggccacg gtttacgcgg gcagcgacga cgggatcaag gtttggatca
      721 acggcgatct ggtgcacacg tgcaaggcgg accgggccct tcaaccggat caggaccgct
      781 tcgacatccg tctcaggaaa ggtcgtaaca cggttcttat caagctcata cagaaccagg
      841 gccagtgggg gctgtccttc cgcatcgacg acccggccga cgagttgcag tatgttgtgc
      901 cctgagttca ttccgcatct ttgccgatcc gttccggttc gcaccatcct tgcaggaatc
      961 gggcgtcatc gatttctcca ggctggatgc ggcgggtatg actttgcctc ggtcgtcatc
     1021 tcgggtgctt cctcgttggc cggagtccgg acgcgactgc ccgcacggtt ctcgacgcga
     1081 gagccgcccg tggcccggct gcccgtcagg atggcaaagc ggacagaatc ggcggaagga
     1141 tcatgatgca gggtccggac gagagcgccg gtgcgtccga gtcaatcgtc caagcggtca
     1201 tcccatctct cgccagactt gcctccagtc ggccgccggt ttgccgaagc ggtcgaggta
     1261 tcgcttgagc acccgcagcc ggtcggtccg gctgacgtgt ggacactctc gcaggtctgc
     1321 gctgagtcga atgagcgcgg ggaggttggc gagcgggtcg cgagagcggg cctggcggag
     1381 gtcgaggtat ccgctcaaaa ccacaacggg cttgtcggca gaaaaccggc taagacatat
     1441 ggtgaggtcc gaggccttca gtttgtcgta aacgaagcct tcctcatcga gcttgcggaa
     1501 caacgcgccg atttggccgg cgatgccggc cctgacgcga tgccgctcct ggggcggcaa
     1561 gcccctcgtc tgttcctgga tgaattcgtg cagcgatcgt gcccctgggg gcttctcgat
     1621 gagaacgaga gcgtatcgga gcaagccgca gcatcgcctt tcgcagacgg ccaaggggcg
     1681 tgcggccggg atgccccggt tgagcagggc gttggctgtt ttccagagtc gcatctcggg
     1741 agacgagaat aagacgtgtt tcagttgccg gccaagggta gggcgcctgc ccaggaggac
     1801 caccgggatg gacttgccgt cttcgagttc cagatcgaat gaaccccggg cgtgttcact
     1861 tctgcttctg cggcagattc tgtctgttcc cggggtggaa agcactctcg atgccagttg
     1921 ccctcgccac caccggcgtg tcaacggcgt cttggggacc gccgagcccg gcgtcgtctg
     1981 tttggtccag aggaacacgc gggctcttgt cctgcggtcg agacggagga tgccgaatcg
     2041 ccggccgttg cgaagagcct ggcggtcgcg tttggcggcc tgggcatttg catgaatccg
     2101 tgctgcgtgc tcaagttctc cgacgagcct gcgccggtct tgaatcaggt tcaggtccgg
     2161 ccggagtcgc ccgcgccacg ccatgtaagc atggagaaac cgcatccggt cggtcaacga
     2221 ggcgttgaag cggaaccagt tgccgaaggc ggccaagtta cggatggccc ggcgattact
     2281 gaggcgacgg cggatggaaa cagcctgcag gtcgacgaag caagcgccat acctgccgtc
     2341 accgtacggg aagaccagaa tgttgccggc atgcaggtct gtgtgcacga aggccccgtg
     2401 atgtgcgcgt gcgatcagct cagccacggc gtcgatgacc gcgtttcggt cgtttcttcg
     2461 caacgaactc ccgatgccgg ctcgaagcca ccaggtgtca agttgttcgg catccggcgt
     2521 ctccattgtg atgaagatac ttgccggttc gcgcccgttt gttgatcgcc acgccgcggc
     2581 cacgggcacg acggtggcga ttccacaccg gatcgcgtgc tcggccgccc gccactcgcg
     2641 caggctgtca gggccgagga tcagacgtcg cagacggtcc aaccaggtta ccggccgggc
     2701 gatcttggcg aacaccgcgc cgcagtctgt ttggacacgg aacacgtctc gaatctcatt
     2761 gcttttgaac agtctcgcgc gggggtcgtt ttggagatcg agccaggaag ggcattgcga
     2821 tgattcgaac agagccagcc agtccgagcg gattcgccag tgccagtcgt cgatgcggcg
     2881 gcggatccat tcgtggggct cgacaagctc tctgttcggc ttgacgcgtg gggcgatgct
     2941 ggctgaggct ggctggtcgg tcatcgccat cgaggtccga tgttcacgca acggccatgc
     3001 gattgagccg gtgcttgatc tcgccgccca cgatggtcat caccgcacgg ccggtgactt
     3061 cccacccgtg gaatggacag ttgcggctct tggagcggaa ttggttcacg tcgatcatcc
     3121 agcggcaatg cgggtcgatc agcgtcacgt cggcgtcggc cccaacggcc agcgtgccct
     3181 tactgattga gagcattttc gccgggcgga ccgtcagtcg gtcgatcatg gcaggccagt
     3241 ccatcaggcc cgtctcgacc agtgccctga tgaacaaggg cagggccgat tccaggccga
     3301 tgatcccgaa cggggcggta aggaactcca gttccttttc ctggattccg tgcggggcgt
     3361 ggtcggtcgc caggcagtcg atcgtgccgt cggcgacgcc tcgccggcag gcctcgacat
     3421 cttccatcgt tcgcagcggc ggtttcatct tgaagttcgg gtcgtaggtg gcgcactgat
     3481 cttctgtcag gacgaggtga tgggcgcata cctcggcggt caccggcaag ccggcggctt
     3541 ttgcctggcg taccagctca acggccctgg cggtggagac gtggcacacg tggtagcggg
     3601 ctccggtggc acggatcagc gtcaggtccc gctggagcat gagttcctcc gcgatggggc
     3661 tgatgccggg gaggccaagc cggacggccg tggctccgcc gttcatgacg ccgccgccca
     3721 cgacatccgg atcctcgcag tgctggagga tcggcttgtc gaacattcga acgtagttga
     3781 gggcctggat catcagttgc gtgttggcca caccgcttcc gtcgtcagag aaggccaccg
     3841 cgccggagcg gaccatctga cccatctcgg cgagctcctt gccttggcgg tctttggtga
     3901 tcgcgccgac cgggtagacg ttgcaggagc cgacccgttg cgactggtgg tagatgaatt
     3961 cgatggaagc ttcgtcgtcg agagtcggct tggtattggg catacaggcc acggaggtga
     4021 atccgcccgc gacggccgct gctgttccgg tctcgatggt ctcttcgtcc tcgtcgccgg
     4081 gttcgcgaag gtggacgtga acgtcgatca gacctgggca gacaatcagc cctgttgcgt
     4141 cgacggtttg agcgcctggc gcgttcaggt tgtggttcat tccggcgacc ttgccgtcgg
     4201 ccaggagcag gtcggcgatc ttatccaggc ctgaggccgg gtcaatgatt cttccgccct
     4261 tgatgaggag ggtctgtgca gccatgggct tcctctgaag ttgggtggct cgttctctac
     4321 cgcacgaccc aacgggacgg cggccggatg attgtacccg aacggggcga gggccggcca
     4381 tcccgggcgg gcggttcctg acaggaggtt ccggggcgat tagactgagc cgcctgaagg
     4441 tgcaaccgcg tactgaggat aatgtgcgat gtctcgtctt gtttgggctt tcttggcggc
     4501 atgggtggcg atgggattga ccatgacgga ggcggtgcta tcgggtgagc cgaccacaca
     4561 accggctccg gtgccggctt cgggccagac tctccgggat ttgcgggcct acctcctgcg
     4621 tcgagcaccg aagttcgtcg ccccgagcga tccgggcgag tggctcccgc aggccgagca
     4681 gattcgtgag aacgttctga ggaatgtcat attccgaggt gtgccgcagc agtggcaaga
     4741 aggtccggtc aggatcgtgt ggcaggagac gctgccgggc aggggctaca agatccgaaa
     4801 gctcaggtac gaggcggtgc cggggctctg gatcggggca ttgctgtacg agcccgaggg
     4861 tcttacgggc aaggttccgg cggtactcaa tccgaacggg catgtcggca agccgggcat
     4921 gaccatcgac tacaagcagg ctcggtgcat caacctggcg aagcgcggca tgctcgcact
     4981 gaactacgag tggatcggca tggggcaact ggacggcccg ggctatgcgc acggcaacgc
     5041 ctcgcacctg gacctgtgcg gccgtgccgg cttgtcggtc ttctaccaat cgctcaagcg
     5101 cgggctggac gtgctcttat ctcatccctc ggccgacccg gagcggacgg ctgtgacggg
     5161 cctgtccggc ggcggctggc agacgattgt tttcagctcg ctggacacaa gggtcaagct
     5221 gtgtgctccc aacgccggtt actgtggcat tgaacggcgc atctgggaga cccgtgacat
     5281 cggcgaccgt gaacagaatc cgcccgacct ggtggctatc gccgattacc cggtgctgac
     5341 agccctgctc gccccgcgtc cggccctgct catctacaac gcgaacgacg attgctgttt
     5401 ttacgcagcg accgcccgct tgtcggtgtt cgagccggtt gcaccggtgt atgaaatgct
     5461 cggcgtgggt gatcacttcg cttttcacgt caacagcgat ccgggcacgc acaattacct
     5521 caaagacaat cgccaggcgt tttaccgttt cattaaccgc catttcctcc cgcccgatca
     5581 gcgcaaggat gaggagatcc cctgcgaaga tgagatccgc aagccggagg aattgaagat
     5641 cgagtatccg ccggatcatg cggacttcta cagtctggcg gcggaagcga tgcgcgatct
     5701 gccggcaaca aagtgcccgg ccggggccga gattgcgcgg aagcaatggt cggacaagac
     5761 gcgcgatgct ctggcgaagg tcgtgcgagc ggatctgact ctcaggacca gcccggtagt
     5821 gacgaagctg tcggtgagca ccatgccggt ggggcagacg cctggaactg ccgtccattt
     5881 gcgcctcgga ggccagtgga cgctgccgat ggtcgagtat tcgccaacga agggtagccc
     5941 gactgtttgc catctgatcg tcgcggatgg cggcatcgga aagacacgtg aggtcgcagc
     6001 gacggcactt ggacgcggcg aacgggtgat tgtggccgac atactgttga ccggtgagtg
     6061 cgtcccggga atcacaggta cgtggcagtt cgcccaaatg atcgatgccg tcggtgcccg
     6121 cccgctgggg atacaggcgg gtcaactcga tgcgatcatt gagcatcttg cctggcggta
     6181 cccgggcgag gtgttacacg tgacgacgat cggccgggtc tcgggtttgg cggccctgac
     6241 gacggccgcg ttgacgcccg gacgcccggc acgactgacg ctccaagata tgatacccag
     6301 cctgaaggac ctgattaccg ggaaggtctc ttggaacagc gctccgtcgc tgttctgctt
     6361 cggcttgttg gaggttgccg acgtgcccga gctgattgaa ctggccaagc cgacccaggt
     6421 gagcatgcaa tgaagacacc cgatctcgac aagcaagccg cgcggcactg ataatgcctg
     6481 cataggaggc ctcatgcgta tcgttgttct ggacggtcac acgatgagcc caggtgacaa
     6541 tccgtgggat gaggtcgcag cgcttgggga gttgacgatt tacgaacggt cgacccacgg
     6601 gcaattgatt gaacgcgcgg gcgcggcgga gatcatcgtc acgaacaagg ttccggtgac
     6661 tgcggccgac ttggatcaac tcgtgaatct aaagatgatc gcggtgacgg ccacgggtta
     6721 caatgtggtt gacgtggagg cggcccgggc ccgcgggatc ccggtctgca acgttcccgt
     6781 ctatgcaaca cagggcgtgg cccagttcac gtttgcactc ttgttggagt tgtgccacca
     6841 cgtcggcgca cacgatgcat cggtcaagca gggcgcgtgg acgcgcagtc cggacttctc
     6901 ctattgcgaa acgccgcaga tcgagctggc cgaccgcgtg atgggggtta tcggctacgg
     6961 gcggatcggc cggcaagcgg cccggattgc acgagccttc gacatgaagg tcatggcctg
     7021 tgacaaagat catgctcccg gtggcgctga tggcgaggtc tgtttcgtcg atatgcggac
     7081 ggtgttctcg caggccgacg tggtgagtct gcattgcccg cagacatcgg agactgcggg
     7141 tctggtaaac gctgagcgcc tgggactgat gaaatcgacc gccttcctca tcaacactgc
     7201 tcgcggcggg ttagtggtcg agcgtgactt ggctgatgcc ctcaataaag gccggattgc
     7261 cggtgcggcc gtcgacgttg tcgccgtcga gccgatccgc gaggacaacc cgctgctgac
     7321 cgccaggaac atcatcatca ccccacacat ggcttgggcg ggcttggccg cccgcaaacg
     7381 cctgatggcc gtcaccgccg agaacatccg cgccttccag cggggaaagc cggttaacgt
     7441 ggtgaactga aggagtcagt gattgcgcga gaagtagtcc cagactttga aaaccttggt
     7501 cacgttgggg ccgcccgcgt tgttatagcg aatgggggtg agctgggcgg cgtgaatctg
     7561 gccctgggga tcgaccacac gcctggcgtg taaatcactg tacaggacgt tgtagctgcg
     7621 gaggtgatag ccctcgtagg gatcgcgatt gtacacctgc ccttcccagg tgaaggcatc
     7681 tttgcccgca aagtagccgt cggagacgaa agcgtgtacg gagtgccggc cgcgcattcc
     7741 ggccgggagc gggccgagga attccggggc ggaatcaacg gcctcgttca ggtactgcca
     7801 gtagggtgat tcgttcgcca gcgacgcgtc gccgtagcgg accgactctt cggggtacat
     7861 ccggctgccg gcgtcgcacg gcgaggcggc ggggatcaga ggtgcggcgt agttgtatga
     7921 gctgaaaggg gagatgggaa atgtgtgcga attctgatat cccggccggc cgcgttttgg
     7981 ggcacggtaa caactcagga actgggcaat gccgttcgat ccggtgctgt cggcggcctt
     8041 gttgttgggg cagtagaaga gttcggggtt cgcacccacg tacttggggt acaacgcgcc
     8101 gatattgacc aaggcccagt cgtgcctgtc gtcgccggaa tacccgaggt agtacttgcc
     8161 ctccatcagt gagaaccgaa acgagccggc ccacggcagg acggtcttgt gatcggcgga
     8221 gtacgttgag agggcgattc cctgctgctt caagttggag aggcagaccg ttgcccttgt
     8281 ttgctcgcgg gttcgagcca gcgtgggcag caggacagcg accagcaggg cgatgatcgc
     8341 aacgaccacc agaacctcga tcagcgtgaa gccggtcagg gttccggcgg ctttgcgcga
     8401 aacccgatca agagttgact gcagcatggc cgctcaccag gcaggatgtt cagcccatcg
     8461 accacatcct acatcgtttt ggctcagttg ccatcaggtt tttgcgatct ccagtctttc
     8521 aaggacggca tcgattgaag tgccactgat ccggacggtc tttcgcggcg agccatggcc
     8581 ggtctgcacc ataacctgcg aggtccgcag gcccattgtc ttggcgagca gtgtgcaaac
     8641 cgcgacgttg gcggcgccct tgtccggggc cgccgcgacg gtgatcttga gcgccccgtc
     8701 gagttcgccc acaatcctgt cgcgggagga tttggggacc accttcacgg gtaagagaat
     8761 atcgttgcct tgtcggcgga tctgaatcat gcccaacagc gtactacgcc aggctgggcg
     8821 ggtcgaggaa ctcgtgatcc gggtcgatg
//
LOCUS       DAOTPW010000241         8807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7235_length_8807_cov_5.293076, whole genome shotgun sequence.
ACCESSION   DAOTPW010000241 DAOTPW010000000
VERSION     DAOTPW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7235_length_8807_cov_5.293076"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..3754
                     /locus_tag="P5316_17805"
     CDS             149..3754
                     /locus_tag="P5316_17805"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HRR86867.1"
                     /translation="MRIDDLGRSCLIVVLVFLSILCACRSAHATVGPGQFLDAAAFAR
                     VCTWDPQRDVGVRAARLQEIAAEDLSLETDLVPDFQGNYSVPPAANGEACIGLQWLEA
                     RPLTMLCVEFKESPPGPGRIQFWQGETVWQGKWIAVDGDLKAEGSSWTFTPAKPGGLH
                     WKVRWIVPTGGKPVVVKSLNARTASTMTTSQIFVQSDPAKPGQKAELELYNGYIVGEQ
                     PSKATSWDMGSSLILPAVNHVSSEAIRQVRADRTMIRFRLPDGAFAVAIDDVLERGPV
                     YLPHVGLFVSKNPPAKSLEEYKRDIADKKSVLQRVREMPDQTRERAMKITHYDPRLDA
                     GRMMISLACDNAKFVTEANGTISYFEHFDRDEKGAKADYVLAPTFGNPRVLGQSQGWG
                     ILGLNKAAHVDPGSAMPLRIREKSYEKGLGHHAPGEIVIDVSEGYDRLEAEVGVQWMG
                     GGTPGSVVFQVLVDGEKKFDSGVMREKDDPKPVSVPLAGARTLTLKLTDAGDGLGFDT
                     ANWCEARLISKGRGGQPVYITELFSETPKLTRHLEGGWLPAPVITGKAGGVEYRQRMY
                     VVPFAKENLPSAPLWLNEEPLCVVELSMKNGDAEPADVGASFVLSMGAADTWPPACET
                     VAHRTVISAGGKLVGVFDSSEFAGGKVRCEKGRLSISGQLPVGGIARCHLYLPAWKMT
                     PDQQGELSGGSELFGRFKAYWEAIMAQGAQIELPDQWIADVIRANQVHILLAARNEQQ
                     GRQVVPWIASDRYWVAIDSEGNSVIRGMQYWGHFDFAQRSFEYFFSHYKPEGYMTMGY
                     TLMGNGWHLWALGEYVRLARDDDWFKSVAETPAGLCRWVMAQLAKTRRLKPDGQKMPE
                     YGLMPPGVQADWNAYAYYFYANSYFRAGLEATGRALKAVGHPDADKIMDAARDLGDEI
                     LRSFRQAQALAPVVPLADGTWVPYYPASAYTPGPMADYYPGQDGNRSWAYDVDLGPHH
                     MVPLGTMPPDAPDVDWIMDHMEDVQFLSDGWGGYPADRNRANWFDMGGFAKVQPYYAR
                     NAEICAMRDDVKPFIRSYFNTLASLIDGTCLSIFEHFSNFCYNKTHETGYFLHQSRTM
                     LLTERGDELWLAPFVTDNWLKDGMTISVKNAPTFFGPVSYEIVSSVGSGHIDAVIDPP
                     ARTRPKAIVLRLRHPERKAIKSVSVDGRSHVDFDPNKETVRVVPADKAIRVEARY"
     gene            complement(3788..5221)
                     /locus_tag="P5316_17810"
     CDS             complement(3788..5221)
                     /locus_tag="P5316_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRR86868.1"
                     /translation="MVENFLSAAVDWVWGPWLLILLLGTHLFLTIRLRFIQRYVFKAI
                     RLSLARKREGSGDISHFGALMTALAATVGTGNIVGVATAVSLGGPGAVFWMWLTGVFG
                     IATKYAEGVLAVKYRITTIDGQMAGGPMYALERGLKQRWLGVAFAFFTAVAAFGIGNM
                     VQANSISEMLAEPMHLPALLGGTEVWMPKWATGIVMTVLTGVVILGGIRSIAHVCGLL
                     VPIMAVCYVAGCLGILIIRYEAVPGALVLIVKSAFSPQAAAGGAVGVGILQTMRYGVA
                     RGLFSNESGLGSAPIVAAAAQTKNPVRQALVSSTGTFWDTVVICLLTGLVIVSSGRWH
                     QGVEGPALTKTAFHALGRIGPLILSVGLLTFVFSTILGWSYYGEKACEYLLGTKAVLP
                     YRIFWVIAIMIGSVVKLQSVWNFADIANGLMAVPNLIALLFLSGVVIAETRKYLWEDR
                     LDEHAEAVAPAGVPVTTVPSAENDGTR"
     gene            5668..6270
                     /locus_tag="P5316_17815"
     CDS             5668..6270
                     /locus_tag="P5316_17815"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRR86869.1"
                     /translation="MGAWLRDHGTLGVGVFITAFAVLAGLALLPTYAQSVLAGWAFGM
                     ATGSVAAMAGIAGASLIGYAVARRGAGDRFVRLIDEQPKWRAVYDALVRSGPGRALLT
                     VTLLRIPHNSPFAITNLVMASCRVPPWIYAVGTVVGIAPRTLLAVYLGARAQTTDFAM
                     PSEKWLYIVWVAGALLAVGVVGAIANQAIKRVTGVEAADG"
     gene            6538..7452
                     /locus_tag="P5316_17820"
     CDS             6538..7452
                     /locus_tag="P5316_17820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRR86870.1"
                     /translation="MERFQRILVCIETLEKARGLLGYASAVSRAVQAREVHLLHVQSP
                     AATRPKAVVNGVAIARRGITTSILRDAAAEIFRGHGREKVQCSVAGGVPLIEILRSAR
                     NRDVDLIILGRRGGEHSGSSLPSRVATKARCSVLVVSDGSEADWKRILVPARKSECSA
                     HAFATACDMGAATGAEVLCLNVYPVRSGYLNVGTSLDEHAALLGDWARHECEELRDNV
                     NSSGANVRIKCVPDFYRKPVAIMLDQVAGEHADLLVIGAQGRRGIAGLLLGAVADKMI
                     RQSDIPVLAVKKKGEAVGLACALRTLAG"
     gene            complement(7612..8631)
                     /locus_tag="P5316_17825"
     CDS             complement(7612..8631)
                     /locus_tag="P5316_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86871.1"
                     /translation="MLDCQGPPGEAGQPIPGTNGLACWDLNADGVQDPDEDVNGDGLW
                     TALDCRGPAGPPGSSGGSGPPGSPGSPGQPGPQLFDLFVNDFIIPDYYYDYEGPYSLN
                     FENGYGYLYAIPTGLPVLEDVDDVTAFAVPVPEYYNAGYPVTLRLFLTAYFRDCAVLQ
                     LNAIRGIPGVGIVPYGQTRWIKLDPPQYAPVFTTAENGYGLGYYLVVDLPVQAGAPDG
                     LALPDDLEKRQMLAFEIRPYELDGSILLGGVEFFESLPGQAGSVIGASIYYEENFESW
                     PESICRLCEIQTEDPDVIRDQYGCPWLCEEDNDCPGEGDVYCTALSDHPDVDACHEMS
                     SSRPS"
BASE COUNT         1659 a         2561 c         2859 g         1728 t
ORIGIN      
        1 tctggatcgc agaacgtttg ggttgcggcc cgcaaaaagg cggtccgcct tagatgttgg
       61 ttgcaggatc agcgtcgact ggacacggcc ggtatcctgc cttatctacc ttgtcagcaa
      121 tcgtctttcc gtttcccgga ggcaaaccat gcgcattgat gaccttggcc gcagttgtct
      181 gatcgtggtt ttggtgtttc tctcaattct gtgtgcctgc cgatcggctc acgccacggt
      241 cggccccggc cagttcctcg atgctgccgc gtttgcccgc gtctgcacct gggaccccca
      301 gcgggatgtc ggcgtacggg ccgctcggct gcaggagatc gcggccgagg acctatccct
      361 ggagacggat ctcgtgcccg atttccaggg caactacagc gttccgccgg cggccaacgg
      421 cgaggcgtgc atcggtctcc agtggctcga ggcccgacct ctcacgatgc tctgcgtcga
      481 gttcaaggag tcgcctcctg gcccgggcag gatccaattc tggcagggcg agacggtttg
      541 gcagggcaag tggattgcag tggacggcga cctgaaggcc gagggcagtt cctggacctt
      601 caccccggcc aagcccggag gccttcactg gaaggtccgt tggatcgttc ccaccggtgg
      661 taagcccgtc gtggtcaaga gcctgaacgc aaggactgcc tcgaccatga ccacttcaca
      721 gatctttgtg cagagcgatc ctgccaagcc cggtcagaag gccgaactgg agctctacaa
      781 cggttacatc gttggggaac aaccaagcaa ggcaacgtcg tgggacatgg gttcgtctct
      841 gatactgccc gccgtcaacc atgtttcttc ggaggccatt cgccaggtgc gcgccgatcg
      901 gacgatgatc cggtttcgcc tgcccgacgg ggcctttgcg gtggccatcg acgatgttct
      961 ggagcgaggg cccgtctacc tgccgcacgt gggccttttt gtcagcaaga acccaccggc
     1021 aaagagcctg gaagaataca agcgggacat cgcggacaag aaaagcgttt tgcagcgggt
     1081 ccgagagatg cccgaccaga cccgcgagcg ggcgatgaag atcactcact atgatccgcg
     1141 cctggatgcc gggcggatga tgatttcgct ggcctgcgac aacgccaagt ttgtcaccga
     1201 agccaacggg acgataagct attttgagca tttcgacagg gacgaaaagg gcgctaaagc
     1261 cgactatgtt ctcgcgccga cgttcggcaa tccgcgcgtt ctgggccagt ctcagggctg
     1321 gggcattctc ggcctcaaca aggccgccca cgttgacccg ggcagtgcga tgcctctgcg
     1381 catcagggaa aagagctacg agaaaggctt ggggcatcat gctccgggtg aaatcgtcat
     1441 tgatgtgagc gaaggctatg accgcttgga ggccgaggtt ggcgtgcaat ggatgggcgg
     1501 cggtacaccc ggcagtgtgg tgttccaggt attggtggac ggcgagaaga agttcgacag
     1561 cggggtcatg cgcgagaagg acgaccccaa gccggtcagt gtgccgttgg ccggcgctcg
     1621 tacgttgacg ttgaagctga ccgatgccgg cgacggtctg ggtttcgaca cggccaactg
     1681 gtgcgaggcc cggctgatca gcaaagggcg gggggggcag ccggtttata tcacggagtt
     1741 gttttccgaa acgcccaagc ttacgcgtca tctggaaggc ggatggttgc ccgctccggt
     1801 gatcactggg aaagcgggtg gcgtcgagta tcggcaacgg atgtacgttg tgccgtttgc
     1861 caaggagaac ctcccgagcg cgccgctgtg gctgaacgaa gaaccactct gtgtggtcga
     1921 actgtccatg aagaacggtg atgcggaacc cgcggacgtt ggggcatcct ttgtcttgtc
     1981 catgggcgca gccgacacct ggccgcccgc ttgcgagacg gtcgcacatc gcacggtcat
     2041 ctccgccggc ggcaagctcg tgggggtgtt cgatagcagc gagttcgctg ggggcaaggt
     2101 tcgttgtgag aaaggtcgtc tttccatatc cgggcaactg ccggtcggcg gcatcgcccg
     2161 ctgccatctg tatctgcccg cgtggaagat gacgccggat cagcaggggg aattgtccgg
     2221 tggttccgag ttgttcggtc gtttcaaagc ttactgggaa gcgatcatgg cccaaggcgc
     2281 gcagatcgag ttgccggacc aatggatcgc cgatgtcatt cgggccaacc aggttcatat
     2341 cctcttggcg gcgcgcaacg agcagcaggg caggcaggtc gttccgtgga ttgcctcgga
     2401 ccggtattgg gtggccatcg acagcgaggg caactcggtc attcgcggta tgcaatactg
     2461 gggacatttc gatttcgccc aacggtcttt tgagtacttc ttcagtcact acaaacccga
     2521 aggttacatg accatgggct acacgctcat gggcaacggc tggcatctgt gggctctggg
     2581 cgaatacgtg agacttgcca gggacgacga ttggttcaag tcggtggctg aaacgccggc
     2641 ggggttgtgc cgctgggtga tggcccaact cgccaagact cgcaggctca agcccgacgg
     2701 gcagaagatg ccggagtacg gcctgatgcc gccgggcgtc caggccgact ggaacgcgta
     2761 cgcgtactat ttctatgcca acagttactt ccgggcgggt ctggaggcga ccggccgtgc
     2821 gctcaaggcc gtggggcatc cggatgcgga caagataatg gacgcggcca gggatctggg
     2881 tgatgagatc ctgcgatcat tcaggcaggc tcaggccctc gcaccggtgg ttccgctggc
     2941 cgacggcacc tgggtgccgt attaccccgc gtcggcgtac acgccgggcc cgatggccga
     3001 ctactacccg gggcaggacg gcaatcgcag ttgggcctat gacgtggacc tggggccgca
     3061 tcacatggtc ccgctgggga cgatgccgcc ggatgcgccg gatgtcgact ggatcatgga
     3121 ccacatggag gacgtgcaat tcctttcgga cggctggggt ggttatccgg ccgatcgcaa
     3181 ccgcgccaac tggtttgaca tgggcggttt cgccaaggtg cagccgtact acgcccggaa
     3241 cgcggaaatc tgcgcgatgc gcgacgacgt gaagccgttt attcgcagct acttcaacac
     3301 gctggcctcg ctgatcgacg ggacgtgcct ctcgatcttc gagcacttct caaatttctg
     3361 ctacaacaag acacatgaga cgggctactt tctgcaccag tcgcggacga tgctgctgac
     3421 cgagcggggc gacgaactct ggctggcgcc cttcgtgacc gacaactggt taaaggatgg
     3481 gatgacgatc agcgtgaaga atgccccgac attcttcggg ccggtcagct atgagatcgt
     3541 ctcgtcggtc ggcagcgggc acattgacgc cgtgatcgat ccgccggccc ggacaaggcc
     3601 gaaggctatc gttcttcgcc ttcggcatcc ggaacgtaag gcgatcaaat cggtcagcgt
     3661 cgacggccgt tcgcatgtcg acttcgatcc gaacaaggag acggtgcgag ttgttcctgc
     3721 ggacaaggcg atccgggtgg aggcccgcta ttgacgtcct ggcgcggcct gccgagcgac
     3781 cagttcatta ccgggtgccg tcgttctcgg cggatggtac agtcgtcacg gggacgcccg
     3841 cgggggcgac tgcctcggca tgctcgtcga ggcggtcctc ccagagatac ttgcgcgtct
     3901 ccgcgatgac gacgccgctc agaaacagca gcgcgatcag gttgggaacg gccatcagac
     3961 cattggcgat gtcggcaaag ttccagactg attgcagctt gaccactgac ccgatcatga
     4021 tcgcaatgac ccagaagatg cggtagggga gtaccgcttt ggtaccgagc aggtactcgc
     4081 atgccttttc accatagtat gaccagccca gaatggtgga aaacacgaac gtcagcaagc
     4141 ccaccgacag aatcagcggt ccaatgcgtc cgagggcgtg aaaggcggtt ttggtgaggg
     4201 ccgggccctc gacgccttgg tgccaacgtc cggaactgac gatgaccaag ccggtcagaa
     4261 gacagatcac caccgtgtcc cagaaggtgc cggtggagga gaccagtgcc tgtcgcaccg
     4321 ggttcttcgt ttgggcggcg gcggcgacga tgggagcgct acccaatccg gattcgttcg
     4381 aaaaaagccc gcgggccaca ccataacgca ttgtttgcag gatgccaacg ccgacagcgc
     4441 ctcctgccgc agcctggggg gagaatgcgg atttcacgat cagcaccaac gcccccggaa
     4501 cggcctcgta ccggatgatc aagattccca ggcaaccggc cacgtagcag acggccatga
     4561 tcggaacaag gagcccgcac acgtgggcaa tcgatcgaat ccctccgagg atgaccacgc
     4621 ctgtcagcac agtcatgacg ataccggtgg cccacttcgg catccagact tcggttcccc
     4681 ccagcaaggc cggcaaatgc ataggctcgg cgagcatttc ggaaatggaa ttggcctgga
     4741 ccatgttgcc gatgccgaaa gctgcaaccg cggtgaaaaa cgcgaacgca acgccgagcc
     4801 accgctgctt cagcccccgt tccagagcgt acatcggtcc cccggccatt tgcccgtcga
     4861 tcgttgtgat acggtactta actgccagga caccttccgc gtatttggtt gcgatcccga
     4921 atacccctgt caaccacatc caaaagacgg cccccggacc gcccagcgac accgccgtag
     4981 ccacgcccac gatgtttccc gttccgacgg ttgcggccaa ggcggtcatc agtgccccaa
     5041 agtggctgat atcgccactg ccttcgcgct tgcgggcaag agacagccgg attgccttga
     5101 agacatacct ttggatgaat cgcaaccgta tggtcagaaa caagtgggtt cccagcaaca
     5161 ggatcagcag ccacggtccc cagacccaat cgaccgcagc cgacaagaag ttctcgacca
     5221 atctgagcca attgctcagt tgttgcggga gacttgcaat gataggcacg ccggcacgtc
     5281 ctttcgtttg tgtaccgcag accgaggctc acgccgcgat gaacatgcga tatgctcttg
     5341 agatcggcca tccggcggcg acagcgtgac tccgagagcg agaaaagtcg gcagacgtgt
     5401 tgcccgcgcc caatgccaga gccgattgac gccaccgctc gttgcgtcta acctcacggg
     5461 atggacgatc agccttccat accgaacggt cctcggcaga cggggtcaga gccggtcgcg
     5521 cacgagggcc gaccgagcga ccggccgtat acctttgaag acctcaagcg tggtttccgg
     5581 cgtctgggcc ccatgggtcc gatggccatc attgcggcca gccttccggc catcgggggc
     5641 ttcgcggtat tggggacgat gggcccgatg ggtgcttggt tacgcgatca tgggaccctg
     5701 ggtgtgggcg tgttcataac tgcttttgcg gtattggcgg ggctggcttt gctgccgacg
     5761 tacgctcagt cggtgctggc cgggtgggca ttcggcatgg ccacgggctc ggtcgcggcc
     5821 atggccggta tcgcgggggc ctcgctgatc ggctatgcgg ttgcgcgccg tggggccggc
     5881 gatcgatttg tcagactgat tgatgagcaa ccgaaatggc gggcggttta cgatgccctg
     5941 gtccggtcgg ggcctggcag ggcactgctt acggttaccc tgttgcgcat accgcacaac
     6001 tcgccttttg ccatcaccaa tctggtcatg gcttcatgcc gtgtgccgcc ttggatctac
     6061 gcggttggaa cggttgtcgg gattgctccc cgaacgttgc tggccgtcta cttgggagcg
     6121 cgcgcccaga ctaccgattt tgccatgcca tcggaaaagt ggctttacat cgtctgggtg
     6181 gccggggctc ttctggccgt cggcgtcgtt ggggcaatcg cgaaccaggc catcaaacga
     6241 gtgacgggcg tggaggccgc agacggataa tcgtcgaagg gggatccgcc ggcgctttac
     6301 ccatctgaag ctaaaccagt cttctttttc catcgatttt agtagcgcca agctctggag
     6361 atggcagcgt ccgcgtccgt gctttggcgt tcaaggtagc tggtctggat ttccggcatg
     6421 accgggctgc ggcgctgcgt ctgtccatga gaggttgcgc gacggggcac gtagcgattc
     6481 tagagcgttg tgccgcctcg aagggagggc ggggccgttc gagaaggaag gttggccatg
     6541 gagcggttcc agcggattct tgtctgtatc gagacacttg agaaagcccg cgggctgtta
     6601 gggtatgcgt cggccgtctc gcgtgccgtc caagctcgtg aggttcacct gctgcacgtg
     6661 cagtcgcccg cggctacccg accgaaggcg gttgtcaacg gcgtagccat cgcaaggaga
     6721 gggatcacca cgagcatttt gcgcgatgct gcggcggaga tcttccgggg gcacgggcgc
     6781 gagaaggtgc aatgttcggt ggcaggcggg gttcccctta tcgagattct tcgcagcgcc
     6841 cgcaatcggg acgtggatct gatcattctt ggtcgtcgcg gcggcgagca ctcgggcagt
     6901 tcccttccca gccgggtggc gacgaaggcc cgctgttcgg ttctggtggt ctcggacggg
     6961 tccgaagccg attggaagcg tatacttgtt cctgccagaa aatcagagtg ctcggcccac
     7021 gctttcgcaa cggcgtgcga catgggtgcg gcgacggggg ccgaggtgct ttgtctgaac
     7081 gtttacccag tccgcagcgg ctatctgaat gtcgggacgt cgctcgacga gcacgcggcg
     7141 ttgctcggtg attgggctcg gcacgagtgt gaagaactgc gtgacaacgt caattcgagc
     7201 ggagccaacg tcaggatcaa gtgcgtgccc gatttctacc gcaagccggt agcgatcatg
     7261 ctggatcagg ttgccgggga gcatgccgac ctgttggtga tcggggccca gggtcgtcgc
     7321 ggcatcgccg gtctgctcct gggagcggtt gccgataaga tgattcgtca gagcgacatt
     7381 cccgtgcttg ccgtgaagaa gaaaggcgaa gccgtaggct tggcatgtgc gcttcgtacc
     7441 ctggccggct gaggccgaca tatacggccg ggaggcggca gtgacgcacg ggctcgggag
     7501 tggtcgttcc cgagcccgct gcatgaatac tgcactccgt cagggcaaat cagaccacaa
     7561 ttctcgtggc gcgcgtgacg ttcaggtgat cggaaagcgc catccaggcc gtcatgaggg
     7621 ccgggatgaa ctcatttcgt ggcacgcgtc gacgtccggg tgatcggaga gggcggtgca
     7681 atagacgtct ccttcgcccg gacagtcatt gtcttcttcg cagagccaag ggcagccgta
     7741 ctgatcgcgg atcacgtcgg ggtcttcggt ttgaatctcg cacaatcggc agatcgattc
     7801 gggccagctt tcgaagttct cttcgtaata gatgcttgca ccgatcaccg atccggcctg
     7861 tcccggcaac gattcaaaga actcgacgcc acccaagagt atggacccgt ccagttcata
     7921 gggtcgtatt tcaaacgcca gcatctggcg tttttccagg tcgtcaggga gggcgagccc
     7981 gtcgggagcg ccggcctgaa ccggcagatc aaccaccagg tagtatccca agccgtagcc
     8041 gttttccgcc gtggtgaaga ccggtgcgta ctgcggcgga tcgagtttga tccatcgggt
     8101 ctgaccgtac ggcacgatcc ccacgcccgg gattcccctg atcgcgttga gctggaggac
     8161 cgcacagtcg cggaaatagg ccgtgaggaa gagtcttagt gtgaccggat aaccggcgtt
     8221 atagtattcc ggtacgggca cggcaaatgc ggtgacgtcg tcgacgtctt cgaggacggg
     8281 tagtcccgtc ggaatggcgt acaggtagcc gtagccgttc tcgaagttga gggagtaagg
     8341 gccttcgtag tcgtagtagt aatcgggaat gatgaagtca ttgacgaaga gatcgaacaa
     8401 ttgcggtcct ggctgtcccg gcgatccggg cgatccagga gggcctgatc cgcctgatga
     8461 gcccgggggc ccggccggcc cgcggcagtc gagcgcggtc cacagcccgt cgccgttcac
     8521 gtcttcatcg gggtcctgga caccgtcagc attgaggtcc cagcacgcca ggccgttggt
     8581 gccgggaatc ggctgaccgg cctcccctgg aggtccctgg caatccagca cgtcaacaac
     8641 cccgtcttga tttctgtcct cttgtggatc agcgttgccg ttggcattca ggtcccagca
     8701 ggagactgcg atccggccgc tggaatcgcc cggttggccg ctgaggagga ccctagcccc
     8761 tgatcaagca tactccggcg aggttttctc gctttgttcg taaaaaa
//
LOCUS       DAOTPW010000242         8796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7241_length_8796_cov_3.970255, whole genome shotgun sequence.
ACCESSION   DAOTPW010000242 DAOTPW010000000
VERSION     DAOTPW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7241_length_8796_cov_3.970255"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="P5316_17830"
     CDS             complement(<1..944)
                     /locus_tag="P5316_17830"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86872.1"
                     /translation="MNLPTLDVIVILLYFGVVTAIGLLSARRVGTQKEFYVGGRRFGK
                     LLMIMTSFGVGTSNTHPILVSGVTYTHGMSGIWYSWLYMLYMPIQWVIEPLIRRLRLY
                     TTADFFDLRYSKGLGLFFSVSTLLNAAVMIGTILIGMGQIMEAVTQGALSKEVVIPTA
                     AAIIIIYGTAGGLVAAAWTDVLQGILIIVLSFMLLPPLFREVGGLAGLHEALPAQAFS
                     LAWPAGDDAARSIGLFAIFMLFVNGMLGALIEPQSQTKQAANDEWTLRLGVQIGTIMK
                     RICTVAWALVGLCAAAIWPNLQNPEMAFGLATRSYLPDG"
     gene            complement(941..1093)
                     /locus_tag="P5316_17835"
     CDS             complement(941..1093)
                     /locus_tag="P5316_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86873.1"
                     /translation="MNESGNQPPLTRPSLRYFMPYQQTAIGFFVVVACVAALILGVKW
                     LLGDPS"
     gene            1634..2731
                     /locus_tag="P5316_17840"
     CDS             1634..2731
                     /locus_tag="P5316_17840"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR86874.1"
                     /translation="MTPVVRGSKARFLKESLLREMTEQRIPIGARLPSESELMARFAV
                     SRTTVRQALAELTNEGFIAKRQGRGTFRISPEERRRRGERSMLVGVWFNWPSGPLYGP
                     MLDGIRSELVHWGYHAVLEGGLETGAEKQGISSLIRKGLDGFIVSPSSDPSDSHQPIV
                     EILERRLPLVLIDKRLPGCEADLVATNNRLGAEQLMSHLLALGHRRIGFIRTANVSTV
                     EERLQGYRFMLHKHGQPIDPVWTKVSDDVYQDHGRTAARELLSLPSDRRPTAVFGAND
                     PIAETVAAVAAELGLRIPADLSVVGFDAAGFNLEQPSWLTTYAQPKYRIGQQAAQLLM
                     RRIHNPVPHIETVFLEGELIQRQSTAPPREP"
     gene            2751..3785
                     /locus_tag="P5316_17845"
     CDS             2751..3785
                     /locus_tag="P5316_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR86875.1"
                     /translation="MAVHTATIGTLGCPGSVKKARRNISHSSRVGFTLIEVLVVVVIL
                     ALLIAVLLPSLRQAREQARLVACQANSKQIAAALAQYRSEENDYVPVVFNYAASVLGA
                     WNPPARACWVSVALRKYDQGTNHLGSRYGGQFDPETVWDRATKDAYEAKAMPEQYACP
                     FERGRGPGHETWRDEGFFRFYEKQGRFESFQTWLWENIVPQRLPAHGVAWPSGKGAAG
                     DGLPRFASFSWNKIRVPQELRTTFADGLPVPSIEGSLLPGGRPNDPSKSACRRWTMGD
                     VKRLRSGSMSTSAVAFCAAGEHILGDQPNQLIGRANVGSHRRSGAGGTNVVFADTHVE
                     WVRGTQIGWP"
     gene            3883..6348
                     /locus_tag="P5316_17850"
     CDS             3883..6348
                     /locus_tag="P5316_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86876.1"
                     /translation="MRWGPMTMNAVGVLGVMAAVGVAYGESFLEDFENPAIYPIGWHL
                     RDTDPWWGQSGTSDDPIIQAGAGVGGSVGLSAAARNFHWVARPFEWKDPTIAGVIIRM
                     DFQTPGNTGDSYWPFRDKIGWTTNQFSTDSAEWLQIELYKNQIRGSWKNASGSDRTPT
                     IVTYDVTPGVTPMTANTFYRAAFKLTKLTMTSAKIDVSLHTINPDGTTGSEVVSGTIA
                     DTDALASDTPNPALFTAPAMWPTYRNYNNGASAKCDNAYFEIIRISAPDCFSKVTPEE
                     AQGVETDINAAPTPSSINYTVQNLGLNTFTYTVVELDDQQQPVDVSWISINKTGATVA
                     AQTSDTLTVSFDSAGLAGGSYTAYLKFTDDCDPAVEHIRQINLSVYGCRWTLDSCSQE
                     RAYLLDYPAKLPEDVVYRLTNTGAYPVNYTVAKSGDSSCFEWLTVMNPTGTVEVGGYV
                     DIVAHIDVAALAGHDTDEDYECTLTFTDNSSPQVVTRDVKLRYLGVGDTQVFMYDGST
                     LPTNDDSAGPGLRFDVDDGEFNGAVEPDPDADNHYVWRLIDPGNDIKTWYRAKFFNPE
                     SSNWERVRERGSVGSTILARLKLRSWGPAGSTRRAMLRIAETDASAADFHWGGLDGVA
                     AETKRGVMVQTGMGTDGFVTLRLTSKGSEEDAAWDCGRIVRLYLNENPTPVIEIINAM
                     AKGTGASGDRGFWFGDSSGDQTIDIAFDWVSGTNAGAFAPGEEVAVLGKSLVPVFGPG
                     QVCNVPFADIDGDGDVDQTDFAAFQRCYMSSPFDWDNCHCLDRAGNQDVIDSDDLWAF
                     EACATGPGVAFDVDRPPENCVPY"
     gene            6440..7288
                     /locus_tag="P5316_17855"
     CDS             6440..7288
                     /locus_tag="P5316_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86877.1"
                     /translation="MTILKWLVVTVVIGVTGAGVAGAAIFIEDFESGYTDGDNIAAYA
                     PDWYGDGATNSPTIQAGQGLAGSVGLSEAKNCFNWSAHPFTWTDPSVTGVIIGMDFQT
                     PDIMDAAFPFKDDRIGWTIDPASSSTSSIFSVQLSQNGTGGFDIEGYFKDGSKTRTDT
                     IISYTTPVQANAWYRFQVKYTKLTNTSARIDATVWELDASGAQGNMIVTGTIADSSAS
                     SGDWTHRPPEALFTAATMCPSFKNYNQGANAKADNAYFEIIPEPTTIGLMLGGVFLMM
                     RPRRRR"
     gene            7413..8477
                     /locus_tag="P5316_17860"
     CDS             7413..8477
                     /locus_tag="P5316_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR86878.1"
                     /translation="MNGMLKTRGFTLIEVLVVVAIIALLVAILLPALSHARNEARSAL
                     CASNMKQAVSGVILQMAETQMRREVWSTNFGWAVYSQKQLKGQTDVFTCPSDENPFPV
                     PGVLDRLYSGGRYRGTTTGDGIFNRVLRDGSGWVTDIQDQVDEDEFGGDAYDDPNGDL
                     LVKHNATKGQHFADATICKGKATWRHDVYTYKGELIAQDVGGGVHVTVPILWMSYGAN
                     ASAGLKNVKGTPILVAEAGKPGIFPENLHSYPSDHLGWALRFRHGERTEKPAVAGANW
                     TAWTLGGRPPQTGGNLPASWIDSHYTPRSRMNAGFLDGHVDRLGYWQVMDINRFTPTG
                     RPAPKSPLWLGTRRSDSISF"
     gene            complement(8457..>8796)
                     /locus_tag="P5316_17865"
     CDS             complement(8457..>8796)
                     /locus_tag="P5316_17865"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRR86879.1"
                     /translation="TTYSNRRNLCTFRTTLAPEDVSDGDLMTADAIAAKTSGHARGEV
                     PIGCTHLTMFIDVQAKALFWLVVAWEADFTGYVIDYGTEPVPEVSASHRQIVPASATR
                     ETQRSERNRV"
BASE COUNT         1839 a         2545 c         2689 g         1723 t
ORIGIN      
        1 ccgtccggca ggtaggaccg agttgccagt ccgaatgcca tttccgggtt ttgaagattg
       61 ggccagatcg cggcggcgca caagccgacc agcgcccatg ccaccgtgca gatgcgtttc
      121 atgatagtcc cgatctgaac gcccagtcgc agcgtccact cgtcgttagc cgcctgtttg
      181 gtctgagatt ggggctcgat cagcgcaccc agcatgccgt tgacaaagag catgaagatg
      241 gcgaacagcc cgatgctcct ggcggcgtca tctccggcag gccacgccag cgagaacgcc
      301 tgtgcgggca gagcttcgtg gagcccggca agcccgccca cctccctgaa cagcggcggc
      361 aaaagcatga acgacagaac aatgatgaga atgccctgca gcacatcggt ccacgccgct
      421 gccaccaggc ctcctgcggt accgtaaatg atgatgatcg cggccgcggt gggtatgact
      481 acctccttgc tcagcgcgcc ttgcgtcacc gcctccatga tctgccccat gccgatcaag
      541 atcgtcccga tcatgaccgc ggcgttcagc agcgtgctca cactgaaaaa aagacccaag
      601 cccttgctgt aacgtaaatc gaaaaagtcc gccgtggtgt agagccgcaa ccgccggatc
      661 agcggctcga tgacccactg gatcggcatg tacagcatat acagccaact gtaccagatc
      721 cccgacatgc cgtgcgtgta cgtcacacct gatacgagta tgggatgtgt gttgctggtt
      781 cccaccccga aagacgtcat gatcatcagc aacttgccga aacggcgccc cccgacgtag
      841 aactccttct gggtgcccac ccgcctggca gatagcaacc cgatcgcggt gaccacgccg
      901 aagtacagca ggatgacgat gacgtcaagc gtcggaaggt tcatgatggg tctcccaaga
      961 gccacttgac gcccaggatc aacgccgcca cgcaagcgac taccacgaag aaaccgatgg
     1021 ccgtctgctg atagggcatg aagtaccgca ggcttggacg ggtaagcggt ggctggtttc
     1081 ccgattcgtt catctgacaa cctcacaatt ccgttgctcg gagactcgcg ggcgcatcgc
     1141 gcgcggcata agctgtgcct tcggccactt tgccgtcatg ccgttggccg gctcaacgat
     1201 acctcccgca catcgcatga aatcagagct catgctagcg acccgaggtt ccccaactgc
     1261 ccgaagatag tccgcacggc tggcgtcggt ggtctggctg gcgggaatcc cccggactac
     1321 ccgtaggcac tcctctggat cccagtttgc ctgcccagat tcctcactac gtcgcaagcg
     1381 cttaagttag catgccgagc ggcgacagtc aatacagctt gtagctatgt gttattagga
     1441 tataacaaca tcatgttgtc tcggctacga tcatataagt ggttttatta cagttgttta
     1501 tggctgtcaa aacctatcta cgagccagcc aggccacgcg caagaaccgg caaaatggaa
     1561 tcttcaaata atgcaaacga aaaattcata acgacgtctt gaccggcttg tcttaatgtg
     1621 ttaagatgat gtcatgacac cagtagtaag gggatcaaaa gcgagattcc tgaaagagtc
     1681 tctccttcgc gagatgaccg agcagcgaat ccctatcggg gctcgcttgc cgtcggagag
     1741 cgagttgatg gcccgcttcg cggtcagccg aacgacggtc cgccaagcgc tcgccgagtt
     1801 gaccaacgaa ggatttatcg cgaaacggca aggtcgaggc acattccgga tctcgccaga
     1861 agaaaggcgc cgacgtggcg agcggtcaat gctggtggga gtgtggttca actggccgtc
     1921 aggcccgctg tatgggccca tgctcgacgg tatccgcagc gaattggtgc actggggtta
     1981 ccatgccgtc ctggagggag ggctggagac gggagcagaa aaacagggaa tatccagttt
     2041 gatacgaaaa ggtctggatg gcttcatcgt atcgccttca tccgacccct ctgattcgca
     2101 ccagcccata gtcgagatcc tcgaacgtcg gttgccactg gtcctgatcg acaaacggct
     2161 gccgggctgc gaggctgatc tggtggccac caacaaccga ttgggagccg agcaactaat
     2221 gtcacatctt ctggctctcg ggcatcgacg gatcggattc atcaggacgg caaatgtctc
     2281 cacggttgaa gagaggcttc agggatatcg tttcatgttg cataagcatg gtcagccgat
     2341 cgatccggtc tggaccaagg tcagcgacga cgtgtatcag gaccatgggc gcacggctgc
     2401 tcgcgagttg ctttctttgc cgtccgatcg tcggccgacc gcggtttttg gggccaacga
     2461 tcctattgcg gagacggtgg cggcggtcgc agcagaactc ggtctgagaa tccccgcaga
     2521 tctgtcggtc gtggggttcg atgcggcggg tttcaatctg gaacagccgt cgtggctcac
     2581 aacctacgct cagcccaaat accgtatcgg ccagcaggcc gcacaattgc tgatgcggcg
     2641 gattcataac ccggttccgc atattgaaac cgtttttttg gaaggcgaac tcattcaaag
     2701 acaatcgacg gcacccccga gggagccgta ggcgtggcag gagaggcagg atggcggtac
     2761 acaccgcgac tattggcacg ttgggttgcc ccgggtcggt gaaaaaggcg cggcgcaaca
     2821 tctcccacag ttcacgggtc ggtttcacgc tgatcgaagt gcttgttgtc gtagtaatac
     2881 tggcgttgct catagcggtg ttgttgccgt ctctccgtca ggcacgtgag caggcgaggt
     2941 tggttgcctg ccaggcaaac agcaagcaaa tcgctgcggc gttggcccaa tatcgttccg
     3001 aagaaaacga ttacgtcccg gtggtattca actatgccgc cagcgttctt ggagcgtgga
     3061 atccgcctgc gcgggcgtgc tgggtatcgg tggcccttcg caaatatgac cagggaacga
     3121 accacctggg atccagatat ggtggacagt ttgatccgga gacggtgtgg gatcgtgcca
     3181 ccaaggatgc ttacgaagcc aaagcaatgc cggaacagta tgcgtgtccc tttgaacgcg
     3241 gccgcggacc agggcacgaa acgtggcggg acgaggggtt cttccggttc tacgagaaac
     3301 aggggcggtt tgagagcttc cagacttggc tttgggaaaa catagtcccg caacgcctcc
     3361 cggctcacgg cgtcgcatgg cctagtggca agggcgcggc cggggacggc ctgccccgat
     3421 ttgcttcgtt ttcatggaac aaaatccgcg ttccgcagga actgcggacc acgtttgcgg
     3481 atggtctccc ggtaccctcg atcgagggca gcttgcttcc gggaggccgc cccaacgatc
     3541 cgtccaagag tgcctgtcgc cgctggacga tgggagacgt gaaacgtctt cggagtggga
     3601 gcatgagtac cagcgccgtg gctttctgtg cagccggcga acacatactc ggcgatcagc
     3661 ccaaccaact gattggtaga gcgaacgtcg gtagtcatcg caggtccgga gcaggtggga
     3721 caaatgtggt ttttgccgac acgcacgtgg agtgggtgcg cggcacccaa atcggctggc
     3781 catgagtccg ctcggccgcg acggagcccc agaaaggggc cggcacgtgg ccaatcgtat
     3841 gtagtcgttg aagcgaatct taactggaag gaggagtaaa cgatgcgatg gggcccgatg
     3901 acaatgaacg cggtaggtgt tctgggtgtt atggcggccg tcggggtggc ttatggtgag
     3961 tcctttcttg aggatttcga gaatcccgcg atctacccca tcggctggca cctgcgtgac
     4021 acggatccct ggtggggtca aagcggcacc agtgatgatc cgatcattca ggccggcgcg
     4081 ggcgtcgggg gttccgtcgg cctctctgcg gccgcccgga atttccactg ggtcgcccgg
     4141 ccgttcgagt ggaaggatcc caccattgcc ggcgtgatca tccggatgga cttccaaaca
     4201 cccggcaaca cgggcgattc ctattggccc ttccgcgaca agatcggctg gacaaccaac
     4261 cagttcagca cggattccgc cgaatggctt cagatcgaac tgtataagaa ccaaatccgc
     4321 ggcagctgga agaacgcttc agggagcgac cgaacgccga ctattgtgac gtatgatgtc
     4381 actcccggag tcacacccat gaccgcgaac accttctacc gcgcggcgtt caagctgacc
     4441 aagctgacga tgacatcagc caagatcgac gtctcactcc acacgattaa ccccgacgga
     4501 acgacgggtt ctgaggttgt ctcgggcacg atcgcggata ccgacgcgct cgccagcgac
     4561 accccaaatc ctgcactgtt cacggctcct gcgatgtggc cgacctaccg gaattacaac
     4621 aacggtgctt cggccaagtg tgacaacgcg tatttcgaaa tcattcggat cagtgcgccc
     4681 gactgtttca gcaaggtgac gcccgaagag gcccagggag tcgagacgga catcaacgcc
     4741 gcgccgacgc cctcctcgat caactacact gtccagaacc tgggactgaa cacgtttacg
     4801 tacacggtgg ttgaactgga tgatcagcag caaccggtcg atgtctcctg gatatcaatc
     4861 aacaagacgg gcgcgacggt ggcggcgcag acatctgaca cgctgacggt cagctttgat
     4921 tccgccggcc tggccggcgg ttcgtatacc gcttacctga agttcacgga cgactgcgac
     4981 ccggccgtcg agcacattcg gcagatcaat ctgtcggttt acggatgtcg ctggacgctg
     5041 gattcgtgca gccaggagcg ggcctatctg ctcgattatc cggcaaagct gcctgaggac
     5101 gtcgtctatc ggctcacgaa tacgggagcg tacccggtca actacaccgt cgccaagtcg
     5161 ggtgacagta gctgcttcga gtggctcacg gtgatgaatc cgaccggcac tgttgaggtc
     5221 ggtgggtacg tggacatcgt ggcccacatc gacgtcgccg ccctggccgg ccacgacacc
     5281 gatgaagact atgaatgcac gctgaccttc acggacaact cttcaccaca ggtcgtgacg
     5341 cgcgacgtga agctccgtta tctcggcgtc ggcgacacgc aggtgttcat gtacgacggg
     5401 agcacgcttc cgacgaacga tgattccgcc ggccccggcc tgcggttcga cgtggacgac
     5461 ggagagttca acggcgctgt tgagccggac ccggatgccg acaatcacta tgtctggcgg
     5521 ctgatcgacc ccgggaacga catcaagact tggtatcggg cgaagttctt caaccccgag
     5581 tcgagcaact gggagcgcgt gcgggaacgc ggcagtgtgg gttcgaccat tcttgcccgg
     5641 ctgaaactcc gctcctgggg ccccgccggc agcaccaggc gggccatgct ccgcatcgcg
     5701 gaaacggatg cctctgcggc tgattttcac tggggcggcc tcgatggagt cgcagccgaa
     5761 acgaagcggg gcgttatggt tcaaaccggg atgggcacgg acggcttcgt gaccctgcgc
     5821 ctgacctcga agggctctga agaggacgcc gcgtgggatt gtgggcgcat cgtgcgcctc
     5881 tatttgaacg agaacccgac gccggttatc gagatcatca acgccatggc caagggaacg
     5941 ggcgcctcgg gcgacagggg tttctggttc ggtgacagca gtggcgacca gactattgac
     6001 attgctttcg actgggtgag cggcaccaat gccggggcat ttgcgcccgg agaggaagtc
     6061 gccgtgctgg gaaaaagctt ggtcccggta tttggtccgg gccaggtatg caacgtgccc
     6121 ttcgccgata tagacggcga cggagatgtg gatcagacgg acttcgctgc ttttcagcgg
     6181 tgttacatgt cgagtccgtt tgactgggac aattgccatt gcttggaccg ggcgggcaat
     6241 caggacgtca tcgacagcga tgacctttgg gcgtttgagg cgtgcgcaac aggtcccggc
     6301 gtggctttcg acgtcgatcg tcctccggag aactgcgttc cgtattgagc gaagaagcgc
     6361 agcctataga aagctgcatt caatcgttta tcgtggttgt gccgttctct tgcaaattcc
     6421 tattagagga ggtgtgaaaa tgactattct caaatggctg gttgtgaccg tcgtgatcgg
     6481 cgtcaccggg gccggggtcg ccggcgccgc gatcttcatc gaagacttcg agtccggcta
     6541 cacggatggt gacaacattg cggcgtacgc acccgattgg tatggcgacg gggccaccaa
     6601 cagcccgacg attcaggccg ggcaaggctt ggctgggtct gtgggcttgt ctgaagccaa
     6661 gaactgcttc aactggtcgg cccatccctt cacttggacc gacccgagcg tgaccggcgt
     6721 gatcatcgga atggacttcc agacgcctga catcatggat gcagcttttc ctttcaagga
     6781 tgatcgcatt ggctggacga tcgacccggc ttcgtcgagc acatcgagca tcttctccgt
     6841 ccagttgtcc cagaatggca ccggcgggtt cgatattgag ggatacttca aggatggtag
     6901 caagacaagg accgatacga tcatcagcta cacgacgccg gttcaggcga atgcctggta
     6961 ccggttccag gtcaagtaca ccaagctgac gaatacttcg gcccgaatcg acgccacggt
     7021 gtgggagttg gatgccagcg gcgctcaggg caacatgatt gtcaccggga cgatcgctga
     7081 tagttccgcg tcgtcagggg attggaccca caggccgccg gaggccctgt tcaccgcggc
     7141 aaccatgtgt ccttcgttca aaaactataa tcaaggggca aacgcaaagg cggacaacgc
     7201 ctatttcgag atcatccctg agccgacgac gatcgggctc atgctcggcg gcgtgtttct
     7261 aatgatgcgg ccccgacgcc gcaggtaagc gggaagcatg tggcgccatg ccatcagcga
     7321 ccgcagcgga gtgcgttcgt gcgcgcggat cgcccttctt aaaggccttc agggaatccg
     7381 aagcgagaag ccatgtgtca gggggccgtg taatgaatgg catgctgaag actcgcggat
     7441 tcacactgat tgaggtcttg gtggtggtgg cgatcatcgc cctgctggtg gccattctgc
     7501 tgcccgcgct ttcccacgcg aggaacgagg ctcgtagcgc cctgtgcgcg agcaacatga
     7561 aacaggctgt tagcggggtg attctgcaga tggccgagac ccagatgcgc agggaggtct
     7621 ggagcaccaa cttcggctgg gcggtgtact cacaaaaaca gctcaagggg cagacagacg
     7681 tgttcacctg cccatcggac gaaaacccct tcccggttcc cggcgtgttg gacaggcttt
     7741 actccggggg caggtatcgc ggtacgacca ctggcgacgg catcttcaat cgggtgcttc
     7801 gcgacggtag cggatgggtg acggacatcc aggaccaggt ggatgaggat gaattcggcg
     7861 gcgacgccta tgatgacccg aacggggacc ttctggtcaa gcacaacgcg acgaagggac
     7921 agcattttgc cgacgcgaca atctgcaaag gcaaagcgac ttggcgacac gacgtttaca
     7981 cctacaaagg cgagctgatc gcccaagacg tcggcggcgg tgtccacgtc accgtcccga
     8041 ttctgtggat gagctacggt gccaacgcca gtgccgggct gaagaacgtc aagggcacgc
     8101 ctatcctggt cgccgaggcc ggcaaaccgg gtatcttccc tgaaaacctc cacagctatc
     8161 cctccgacca ccttggctgg gccctgcgct ttcgtcacgg tgagagaacc gagaagccgg
     8221 ccgtggctgg ggccaactgg accgcgtgga cgcttggggg caggccaccc cagactggag
     8281 gcaaccttcc cgcctcatgg atagacagcc actacacacc ccgatcacgc atgaacgccg
     8341 gcttcctcga cggacatgtt gatcgcttgg gctactggca agtaatggac atcaaccggt
     8401 tcacgccgac cggtcgtccc gcgcccaaga gccctctgtg gcttggtact cggcggtcag
     8461 actcgatttc tttctgaacg ttgcgtctct cttgtcgcgg atgcaggtac gatctgccgg
     8521 tggctggctg acacttctgg caccggctcg gtgccgtagt cgatcacgta gccggtgaag
     8581 tcggcctccc atgcgaccac gagccagaac aacgccttgg cctgcacgtc gatgaacatg
     8641 gtcaggtggg tgcagcctat gggcacctcg ccacgggcat ggccgctagt cttcgcggcg
     8701 atcgcatcgg cggtcatcag gtcgccatcg gagacgtctt cgggtgccag cgtcgttctg
     8761 aaagtgcaga gatttcgccg gtttgaatat gtagtc
//
LOCUS       DAOTPW010000243         8784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7252_length_8784_cov_5.377592, whole genome shotgun sequence.
ACCESSION   DAOTPW010000243 DAOTPW010000000
VERSION     DAOTPW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7252_length_8784_cov_5.377592"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="P5316_17870"
     CDS             complement(<1..254)
                     /locus_tag="P5316_17870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR86880.1"
                     /translation="MSDSPVALVTGAGQGIGRGIALRLAREGHIVVINDVVADPANTD
                     HGAYEVKRTIEAAGGRAEVFKADIASAEQRLAMIEFIDLQ"
     gene            676..2415
                     /locus_tag="P5316_17875"
     CDS             676..2415
                     /locus_tag="P5316_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRR86881.1"
                     /translation="MAFLGSVGLKGRAIMLAALLLLGTVGAVCAALMWRHFTSSIEAV
                     EHHAVMNARFIGLSAEPYVLLNDVQSLEHLVQSATTSNCVELTQVLDKTGKVLAESAA
                     KPQFVLETQVRLAQAFRGNLTRESFRVFRTPRQCLALVPIWPVGQQLDLGLDDQEAKQ
                     PGESEPVGFVLLAYTLDDVYQVLAKDAFYSVAITVMLLSVGLAITVVMVRQLVRPIAG
                     LVQTASAIAEGDLSDRASEDAPGEVGILAKAFNNMADKMEDYAQNLEKLVYDRTVALE
                     RNEARTRAILDTAADGIITIDERGIVESYNAAAERVFGWTAAEVIGQNVSMLMPSPYA
                     ESHDSFIKAYLRSGDPQLIGRGREVQGRRKDGSTFPMDLAVSETAVGDRRTFTGIVRD
                     ITERKHAEEEQQKLVSLVENSSDLIMMCSLDGRVLYLNRSGREIAGLDGSEQVAGTTI
                     ADYMPSEEWARVREVELPRVLKSGSAEWHGELQHFKTGTRISMQISFFLIRHPQTGDA
                     ICLGSIQRDITAQKHAERQLRKNMAELEQFNRLAVGRELRMIELKRQVNEMARAAGLD
                     EPYDLSFVEAARK"
     gene            2447..6808
                     /locus_tag="P5316_17880"
     CDS             2447..6808
                     /locus_tag="P5316_17880"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR86882.1"
                     /translation="MGKRFRKILLMRDRWPAWVVLACAVLLAIFARSVAVEQMRLKDR
                     ARFDTEVERIRSVLQERLRQSEHAVRSVCALFESSNQVEESEWEHFAGNALTDDENKV
                     VESLYFVERVPREKLEEYVRQCRAEGAAGFEVHPQGDRPTYMPVRYVFPPKANGIKLG
                     LDMAQDLFWRAAAERARYTGQVEGSIRRPAAQSGNRCPEYRMLKAVYRVSPGQVEGEQ
                     SRRQFIGWVGVCVGGKGLAQGLAAQCRSIGLSIDVFEGISASRETILYASEGEPAVLR
                     SESSPMFEKYAELKVGDSIFTMRAASLPAFASPWSRGIGMAVFLVGTAVGLLMFATLW
                     SRSAARAALAVAERTNQDLRESEGKYRLLFDGSVDAILLLTDVVVDCNAQACKLFGCS
                     RQELTGRSFEDLSPTRQKDGRLSAETAQENIRAALGGTPQFCHWTLKRRDGRTVDTEI
                     WLKSLSMSGRPVLLAIIRDMTERKRAEESVRQSLRMAADIMRAIPSGLFIYGYNPPDD
                     FVLLGGNPEAQRLTGVRLDECLGKSFGELWPELKRDALLDALLNVIRTGNPLEIEELE
                     YRSEIFGGVFRVRAFAMPGNRLGIAFDDVTERHKAEAAARIEAAKLNTILANIDEGVA
                     FAEADDRIIEANPRFVDLVGGSREDIVGRSLWDFRGGSFVEGIRETVNGFRRQTGSQP
                     VTMQQRIKDQDVVLRLQPLYDSDAYAGVLLSIMNVTDMVRAREELERSNREVTERARQ
                     LEEARLASLNMVDDLERARVAAEAANRAKSEFLANVSHEIRTPMNAIMGMTELMLETD
                     LTVDQRESLQIVKESANSLLAVINDILDFSKVEAGRMDLERVPFDLRESLSDTARAHA
                     LAAHKKGLELVCDIAPEVPGMVVGDPVRLRQVVVNLLGNAVKFTEKGEIVVRCDLWNP
                     AASPVGLGPGTGKKLDALSAHSEDRILLHFSVADTGIGIPREKQQMVFEAFAQADGST
                     TRKYGGTGLGLAVSAKLVELMNGRLWVESEPGQGSTFHFVAEFWQSGTSAGPSDSKTV
                     SSLRNLAVLIVDDNETNRCVLERTLHRWNMKPTSASGGEAALKLLHEAKAAGRPFPLI
                     LLDAMMPEMDGFELAKRIRQNPALAGATIMMLSSAKQQEDVARCRELGISVYLTKPIR
                     CSELMDAIMEALGLRSPVPVVQESEPEQAGEAGRRMPRVLLVEDNEVNQKLAVRILEK
                     FGCPLAVANNGKEALEILAREGEDAFDLVLMDVQMPVMGGFEATSAIRERERRTGGHL
                     PIVAMTANAMKGDREQCLAAGMDDYLPKPIDVGALRNLLRKLPCKGSRSRENAMNNEQ
                     TTNETAGVPEPTHGCPVDFTKAMENLGGERELFEEVLGLFVQTIPQIRKDLETAIAAR
                     DAARLRLVAHGLKGSAANVCAESARRAAEEIESMAKQEDFAEIDKVFAVLDAHLKRLQ
                     EYAESRSSQGSSAEQES"
     gene            7090..8106
                     /locus_tag="P5316_17885"
     CDS             7090..8106
                     /locus_tag="P5316_17885"
                     /inference="COORDINATES: protein motif:HMM:NF021627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HRR86883.1"
                     /translation="MADVGEKPAGTVAVPPDELGERVLIQSALPQGQGPPSRVLVAPW
                     GEVKSAKGTFVVDGESAKLVIEAFRAHGTDIPVDYEHQSLGGEYASPSGQAPAAGWIR
                     ALSVVEPGEAAEEPGLFADVEWTEAARKRLSAKEYRYLSPVVLVRKSDRRMVALHSAA
                     LTNKPAIVGMKAIVNNEVSRAADPGEGQSSPHCLSAEADALRRRLGLPEDSDSREILI
                     AAEARLASLAAESVRRGAEERVVEAMRTGKLTQAQRQWALSLAMKHPETFEEWLKTAP
                     VLVVPGRINPPAGEDTANQRRWAVIASARAEFRSRPELALITSEQAWIRNALRDAGLE
                     LSED"
     gene            8178..>8784
                     /locus_tag="P5316_17890"
     CDS             8178..>8784
                     /locus_tag="P5316_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86884.1"
                     /translation="MALTANRDLDRYVDQELRSLPVKGGARIFKGGFVGLSGGYARAL
                     SGGDPFAGIAYEEADNSGGDDGAVMVRVFTTGDFEHVLASASRINNRAAVFASDDGTL
                     TMTAAGNSFVGHQIDAPAANRIVLRIQVTPTPLAGGTMTGELVSAGLRHAFVTKDATG
                     GNVSLAASELGRIVAVNNGTSARNLQLPAVTAGDVGKWVMLI"
BASE COUNT         1683 a         2457 c         3025 g         1619 t
ORIGIN      
        1 aactgcaggt cgatgaattc gatcatcgcc agacgctgtt cggcggaggc gatgtccgcc
       61 ttgaaaacct cggctcgccc gccggccgcc tcgatggtcc tcttgacctc gtaggctccg
      121 tggtcggtgt tggccggatc agcaacgacg tcgttgatca ccacgatatg gccttcgcgg
      181 gccagccgca gggcgatgcc cctgccgatg ccctgtccgg ccccggtaac cagtgccacg
      241 ggcgaatcgc tcattggtac accctcagaa tcaggttctg ctcaggtcct tggggcatac
      301 tgtagcggtg atgcagggac gatgccaagg gttgtttccg gacgttaaag actcgggcgg
      361 ccgggattga accacgggag cttgcggtgc cactgcggcc cggccgtgcc catcaggcgg
      421 accgcagtca ttatcgctcg ttgtcgcgtt cctgctgcgg ctgaccggcg cagttcctgg
      481 gcctgcgcgc attctgagac ctgtgtattt gcccttcagg ccaaacatgc cgggccaagt
      541 ggtcgatgca gagacggagc atcgtgcgtc acacgaggcc cggggtcttg tcattgtcca
      601 ctctggcagg atggacccct ggagccgtgt gccgccccgg tctcgtgtcg gcaaggggaa
      661 acgggggcat cccggatggc gtttctcggt tcagtggggt tgaaaggccg cgcgatcatg
      721 ctggcggcgt tgttgctgct gggcacggtg ggagcggtct gcgccgcgct gatgtggcgg
      781 cacttcacca gctcgatcga ggccgtggaa catcacgcag tgatgaacgc gcgcttcatc
      841 gggctgagcg ccgagcccta cgtcctcctg aatgacgtgc aatccctcga gcatctcgtc
      901 cagtcggcga ccaccagtaa ctgcgtcgag ctgacacaag tactcgacaa gacgggcaag
      961 gtgctggccg agtcggcggc caagccgcag tttgtcctcg aaacgcaggt gcggctggcg
     1021 caggcctttc gcggcaacct cactcgcgaa tcgtttcgcg tgtttcgcac gccgagacag
     1081 tgtctcgccc ttgtaccgat ttggccggtg ggccagcaat tggatctagg tctggacgat
     1141 caggaggcga agcagcccgg cgagtcggag cccgtcgggt ttgtgctcct ggcgtacacg
     1201 ctggacgacg tttaccaggt tcttgcgaaa gatgcgttct acagcgtcgc gatcacggtg
     1261 atgctgctca gcgtcggttt ggcaatcacc gtcgtcatgg tccggcagct tgtgaggccg
     1321 attgccgggc tggtacaaac ggccagcgcg atcgccgagg gtgatctgtc ggatcgggca
     1381 agcgaagacg cgcccggcga ggtggggatt ctggcgaagg cctttaacaa catggccgac
     1441 aagatggagg attatgccca gaacctggag aagcttgtct acgaccgaac cgtagccctg
     1501 gaaagaaacg aggccaggac tcgggcgatt ctcgacaccg cggccgacgg catcatcacc
     1561 atcgacgagc ggggcatcgt cgaatcatac aacgcggccg ccgagcgagt gttcggatgg
     1621 acggcggccg aggtcatcgg gcagaacgtg agcatgctca tgccgtcgcc ctacgccgaa
     1681 agccatgatt cattcatcaa ggcctacctg cgatccggtg atccgcaact gatcggccga
     1741 ggaagggaag tccagggacg tcgcaaggat ggcagcacgt tccccatgga tcttgcggtc
     1801 agcgaaacgg ccgtcggaga ccgcaggacg ttcacgggaa tcgtccgcga catcaccgag
     1861 cgaaagcacg ccgaagagga acagcagaaa ctcgtgtccc tcgtagaaaa cagcagcgac
     1921 ctgatcatga tgtgctcgct cgacggcaga gtgctgtacc tcaacaggag cggccgcgag
     1981 atcgcggggc tcgacggttc cgaacaggtc gcgggaacga ccatcgcgga ctatatgccg
     2041 tcggaagagt gggcgcgtgt tcgcgaggtg gagttgccca gggtgcttaa gagcggaagc
     2101 gccgagtggc acggcgagct tcagcacttc aagacgggaa ccaggatttc catgcagatc
     2161 agcttcttcc tgattcgaca cccgcagacg ggcgacgcga tatgtctcgg cagcattcag
     2221 agggacatca ccgcccaaaa gcatgcggag cgtcagctca ggaagaacat ggccgagctc
     2281 gagcagttca atcgtctggc cgtaggccgg gagctgcgaa tgatcgaact caaacgacag
     2341 gtgaacgaga tggcccgggc ggcggggctg gacgagccgt acgacctgtc gttcgtggag
     2401 gcggcacgaa agtagggcag agaacgggta tggtcaggga tctttgatgg gcaaacggtt
     2461 tcgtaaaatc ctgttgatgc gcgatcgttg gccggcgtgg gtcgttctgg catgcgccgt
     2521 tctcttggca atcttcgccc ggtccgtcgc cgtggagcag atgcgactga aagatcgggc
     2581 ccgctttgac acggaggtgg agcgtattcg gtcggtgctt caggaacgac tccggcagag
     2641 cgagcacgcc gtgcggtcgg tctgtgcgct tttcgagtcg agtaatcagg ttgaggagtc
     2701 ggagtgggag catttcgcgg gaaacgccct gactgacgac gagaacaagg tggtggagtc
     2761 gctgtatttt gtcgaacgcg tgcctcgaga gaagctggag gagtacgtcc ggcaatgtcg
     2821 ggctgaaggc gccgcgggat ttgaggtgca ccctcaaggc gacaggccga cgtacatgcc
     2881 ggttcgatac gtctttccgc ccaaggcgaa cgggatcaaa ttggggctcg acatggccca
     2941 agacctgttc tggagggcgg cggccgaacg agcccgttat acgggtcagg ttgaaggcag
     3001 catcaggcgt cctgccgccc agtcgggcaa ccgatgcccg gaataccgaa tgctgaaggc
     3061 ggtctaccgg gtgagtccgg ggcaggtgga gggtgagcag tcccgccgac agttcatcgg
     3121 ctgggtcggc gtgtgcgtcg gcgggaaggg attggcccag gggcttgcag cgcaatgccg
     3181 ctcgatcggc ttgagcatcg atgttttcga aggaatcagc gcttcgcgtg agacgatcct
     3241 ctatgcctcc gaaggtgagc cggcggtgct gcgctctgaa tcttcgccca tgtttgagaa
     3301 gtacgcggag ctgaaggtcg gtgattcgat cttcacgatg cgcgcggcaa gcctcccggc
     3361 gtttgcgtcg ccctggagcc gtgggatagg catggccgtg ttccttgtcg ggacggccgt
     3421 cggcttgttg atgtttgcga cgctgtggag caggtcggcg gccagggcgg cgctcgccgt
     3481 ggccgaacgc acgaatcagg acctccgcga gagcgagggc aaataccggt tgctctttga
     3541 cggctcagtg gatgccattc tgcttctcac ggacgttgtg gttgactgca atgcgcaagc
     3601 gtgcaaacta ttcggttgtt cacgtcagga gctcactggc agatcattcg aggacctctc
     3661 gccgaccagg cagaaagacg gtcggctctc ggcggagacg gcccaagaaa acatccgggc
     3721 cgcccttggc ggtaccccgc agttctgtca ctggaccctc aagcgacggg acgggaggac
     3781 ggttgacacc gagatctggc tcaagtctct gtccatgtcg ggccggccgg ttcttctcgc
     3841 gatcattcgc gacatgaccg agcgtaagcg cgcggaagaa tcggttcgac aatcgctgcg
     3901 aatggcggcc gacatcatga gggcgattcc ctcaggcttg ttcatctacg gatacaaccc
     3961 tccggacgat ttcgttcttc ttggcggcaa cccggaagcc caacggctga cgggcgttcg
     4021 cttggatgag tgcctgggca agagtttcgg ggaattgtgg ccggagctga agagggacgc
     4081 gctcctcgat gcgctgctca acgttattcg gaccggcaat ccgctcgaaa tcgaggagtt
     4141 agagtaccga agcgaaatct tcggcggcgt gtttcgggtt cgggctttcg caatgcccgg
     4201 caatcgtctt ggtatcgcct tcgacgacgt aaccgagcgg cacaaggcgg aagcggcggc
     4261 gaggatcgaa gccgcgaagc tgaacacgat cctggcgaat attgatgagg gcgtggcttt
     4321 cgcggaggct gacgatcgga tcatcgaagc caaccctcgt ttcgtggatc ttgtcggcgg
     4381 gagccgcgag gacatcgtcg ggcggtcgtt gtgggatttc cgcggcgggt cgttcgtgga
     4441 aggtatccga gagactgtga acggctttcg tcgtcagaca ggcagccagc cggtgaccat
     4501 gcagcagcgg atcaaggacc aggacgtggt cctgcggctt cagccgctgt acgacagtga
     4561 tgcttatgcc ggtgtgttgc tgagcatcat gaatgtgacc gacatggttc gcgcccggga
     4621 ggaactggag cggtccaacc gcgaggtcac cgagcgtgcc aggcagctcg aggaggcgag
     4681 gctcgcgtct cttaacatgg tcgacgatct cgagcgcgcc cgcgtcgccg cggaggccgc
     4741 caaccgggcc aagagcgaat tccttgcgaa cgtcagtcat gagattcgca cgccgatgaa
     4801 cgccattatg ggcatgacgg aactcatgct ggaaaccgat ctgacggtgg atcagaggga
     4861 gtcgctgcag atcgtcaaag agtcggcaaa ctccttgctc gcggtgatca acgatattct
     4921 cgacttctcg aaggtcgagg cgggcaggat ggacctggag cgtgtcccct tcgatctccg
     4981 cgagagtctc agcgacaccg ccagggcgca cgcgctggcg gcccataaga aggggcttga
     5041 gctggtgtgc gacatcgctc ccgaggttcc cggcatggtg gtgggcgatc ctgttcgtct
     5101 gcggcaagtc gtcgtcaatc ttctaggcaa cgcggttaag ttcaccgaaa agggcgagat
     5161 cgtcgttcgc tgcgatctgt ggaacccggc cgcgtctccc gtcggtttgg gaccgggcac
     5221 cgggaagaag ctcgatgcgc tgtccgctca ttcggaggat cgaattctgc tgcatttctc
     5281 ggtcgcggat acggggatcg ggattcctcg cgagaaacag cagatggtgt tcgaagcgtt
     5341 cgcgcaggcg gacggctcga caacgcgcaa gtacggcggt accggcctgg ggctggcggt
     5401 gtcggcgaaa ctcgtcgagt tgatgaatgg acgactctgg gttgagagcg agcccggcca
     5461 gggcagtacg ttccatttcg tggcagagtt ctggcaatcc gggacgtcgg ccggtccgtc
     5521 cgattccaaa accgtctcca gcctgcggaa tctggccgtc cttattgtcg acgacaacga
     5581 gaccaatcgc tgcgtgcttg agaggacgct gcatcgctgg aacatgaaac cgacatcggc
     5641 aagcgggggc gaggcggccc tgaaattgct gcacgaggcc aaggctgccg gccggccgtt
     5701 ccccctgatt ctcctggacg ccatgatgcc ggaaatggac ggttttgaac tggccaagcg
     5761 catcaggcag aaccctgccc tggccggcgc aacgatcatg atgctgtcct ccgccaagca
     5821 gcaggaggat gtggcccgat gccgggaact gggtatctcc gtgtacctga ccaaacccat
     5881 caggtgctcg gagctgatgg atgcgatcat ggaagcgttg ggcttgagga gcccggttcc
     5941 ggtcgtgcag gaaagcgaac cggagcaggc cggcgaggcc ggacggcgga tgccgcgggt
     6001 cttgctggtt gaggacaacg aggtgaatca gaagctggca gtccgcatcc tggagaaatt
     6061 cggttgtccg cttgcagtgg cgaacaacgg aaaggaagcc ctggagattc ttgcgcgtga
     6121 aggcgaggat gcgttcgact tggtgctgat ggacgttcag atgccggtga tgggcggctt
     6181 cgaggccaca tcggcgattc gtgagcggga gcgccggaca ggcggccatc tgccgatcgt
     6241 ggccatgacg gccaacgcga tgaaaggtga tcgtgaacag tgccttgcgg cagggatgga
     6301 tgattacctt cccaagccga tcgacgtggg ggctctgcgc aatcttttgc ggaagctgcc
     6361 ctgcaagggg tctcggtcaa gagagaatgc catgaacaac gaacagacta cgaacgaaac
     6421 ggcaggcgtg ccggagccga cgcatgggtg tccggtggat ttcaccaagg ccatggaaaa
     6481 cctgggcggg gagcgcgagt tatttgagga ggttctgggg ctcttcgtgc agaccatacc
     6541 ccagattcga aaggatctgg aaacggcaat tgcggccagg gacgccgcaa ggctccgctt
     6601 ggttgcacat gggctgaagg gatcggcggc caacgtctgc gccgaatccg cccgccgggc
     6661 ggccgaggag atcgaaagca tggccaagca agaggacttc gccgagatcg acaaggtctt
     6721 tgccgtattg gatgcacacc tgaaacgatt gcaggaatac gctgagtcgc gttcttccca
     6781 aggctcgtcg gccgagcaag aatcgtgatc ctccgcagcc gcagaaccac ggccctggac
     6841 atcctttctc atttttgagc tgagcctcgg cccgctgaaa cgccggccgt cacgttcaat
     6901 cgccgaatgc accaatcaca tcgagttgct cccttgccgt tgggggggca ttgcccctgc
     6961 tccgttcatg cggcggttgc cggagcaggg cgcgtgattt tgttgcgtca ggtcgggagg
     7021 agccggccgg ccgttggcct ggacggcatg gaataaccgg gcaacccagg tgtgaggatg
     7081 gaggtgcata tggctgacgt cggtgagaag ccggcaggta ccgtcgccgt gccgccggac
     7141 gaacttggcg agcgagttct gattcagtcg gccctgccgc agggacaggg ccccccttcg
     7201 cgtgtgctgg tggcaccgtg gggcgaagtg aaaagcgcga agggaacctt cgtggttgat
     7261 ggtgaatcgg ccaagctggt gattgaagcg tttcgggccc acggaacgga catcccggtc
     7321 gactacgagc atcagagcct cggcggcgaa tacgcgtcac ccagcggaca ggccccggca
     7381 gcaggatgga ttcgcgcatt gtcggtggtc gagcctggag aggctgccga agaacccggc
     7441 ctcttcgccg acgtggagtg gaccgaggct gctcgcaaaa ggctgtccgc aaaggagtac
     7501 cgctatctgt cgccggtggt gctcgtgcgc aagagcgacc ggcggatggt tgcgctgcat
     7561 tcggcagcgc tgacgaacaa accggcgatc gtcgggatga aggcgattgt caacaacgag
     7621 gtctcacgtg cggcagaccc gggtgagggg caatcatcgc ctcactgctt gagcgcggag
     7681 gccgacgcac ttcgacgccg gctggggctt ccggaggaca gcgattccag ggagatcctg
     7741 atcgccgcag aggcgaggtt ggcctcgttg gcagccgagt ccgttcgtcg cggggcggag
     7801 gagcgagtgg ttgaagccat gcggacgggc aagctgacgc aagcgcagcg gcagtgggcc
     7861 ttgtcgctgg ccatgaagca tccggagacc ttcgaggaat ggttgaagac ggctccggtt
     7921 cttgttgtcc ccggtcggat caaccctccg gccggcgaag acacggctaa tcagcgacgg
     7981 tgggcggtga tcgcctcggc gcgggctgag ttccgctcgc gaccggagtt ggctctgatc
     8041 accagcgaac aggcgtggat acgcaatgcg ctgcgtgatg ccgggctcga gctgagtgag
     8101 gattgaagtg agaagatcgt ctcgtcaagc ggttgaccga ttcgcggccg ccgcttgcgg
     8161 agaggaagga gaagaacatg gcgcttacag ccaatcggga cttggacagg tacgtggacc
     8221 aggaactgcg atcgctgccg gtcaagggcg gggcgcggat cttcaagggc gggtttgtcg
     8281 gtctgagcgg cggatacgcc agagcgctgt cgggcggtga tccgttcgct gggattgcct
     8341 acgaggaggc ggacaactcg ggcggcgacg acggggccgt gatggtgagg gtgtttacga
     8401 ccggtgactt cgagcatgtg ctggcctccg cctcgcgaat caacaaccgg gccgccgttt
     8461 tcgcctccga cgacggcacg ctgaccatga cggcggccgg caacagtttc gtgggacatc
     8521 aaattgacgc gccggcagcc aaccgcattg tgctccggat ccaggtcacg ccgacgcctc
     8581 tcgcgggcgg cacgatgacc ggcgagctgg tcagcgcagg gctgcggcat gcgtttgtca
     8641 ccaaggacgc cacgggaggg aacgtcagcc tggcggcctc ggaactcggc aggattgtgg
     8701 ccgttaacaa tgggacctcc gcccgcaatc tccagttgcc tgcggtgacg gcaggcgacg
     8761 tcggcaaatg ggtgatgctg atca
//
LOCUS       DAOTPW010000244         8756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7275_length_8756_cov_5.709459, whole genome shotgun sequence.
ACCESSION   DAOTPW010000244 DAOTPW010000000
VERSION     DAOTPW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7275_length_8756_cov_5.709459"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="P5316_17895"
     CDS             <1..627
                     /locus_tag="P5316_17895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRR86885.1"
                     /translation="IRAAIEMGMVDLGERRVQDLVKRAGMINEHLARRRTLEPGTCPP
                     TPRWHMIGHLQRNKVRQLLPWVDMIHSLDSLRLAEEISDEAAKLGRVVPCLIEINVSG
                     EKSKFGIAVGAAPYLAEQVVAMPGLSIVGLMTMAPLVENAEEARCYFRRLRELFEDMK
                     HEGVVKPEFRELSMGMTNDFEVAIEEGATLVRIGTALFEGLTNPVQQQ"
     gene            782..1966
                     /gene="carA"
                     /locus_tag="P5316_17900"
     CDS             782..1966
                     /gene="carA"
                     /locus_tag="P5316_17900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRR86886.1"
                     /translation="MPNRPCKLALEDGTVFAGEAFGADGTHDGEVCFNTSMTGYQEII
                     TDPSYCGQIVTMTYPLIGNYGITEEDLESRGPQVEGFVIKELARRHSNFRAGGSLEDW
                     LRKHGVVAIEGVDTRAITRKLRIQGALRGSISTEILDDAALVERARSWPGLVGQDMVK
                     RVAPKEAFKWTSGFESKFAMDHRHKTGTYRIVAMDCGMKRNILRNLVEAGCEVTVVPP
                     DTAAEAILEHKPDGIFVSNGPGDPEPLDYAIKSLRGLVGKAPIFGICLGHQLLGLALG
                     AKTYKLKFGHRGGNQPVMNLATRRVEITSQNHGFAVETSSLVKVGGVPTHENLNDKTL
                     EGFSHKDLPIFSVQYHPEASPGPHDATYLFDCFIDMIRSGKAPTAEEMAAAQARLEGK
                     AG"
     gene            2209..2457
                     /locus_tag="P5316_17905"
     CDS             2209..2457
                     /locus_tag="P5316_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86887.1"
                     /translation="MRTVETTAKTGPDGVLRLELPTGQPDQTVHVVVTPQPISETDQQ
                     ARLLKAGIRPPVRWNEEQIRQLDLGDPPVSQTLVEDRR"
     gene            2454..2915
                     /locus_tag="P5316_17910"
     CDS             2454..2915
                     /locus_tag="P5316_17910"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRR86888.1"
                     /translation="MSLYYLDASAWVKCYVREAGSDWMRGFWDLRLPVACSSLGYIEV
                     ISAILRRAGRSDSEIGAVLGKIGRDREAIYEAGVSATVLEQAEALVRRHRLRTADAIH
                     LASAAMMGKELEESVSVIASDAELLAAATAEGMAAIDPQANPQLPTGKPQA"
     gene            2912..3139
                     /locus_tag="P5316_17915"
     CDS             2912..3139
                     /locus_tag="P5316_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86889.1"
                     /translation="MKRYSRPLLRAGVKQKRRKNHRVEPASCRSATGWKPGPHDATYL
                     FDCFIDMIRSGKAPTAEEMAAAQARLEGKAG"
     gene            3328..4032
                     /locus_tag="P5316_17920"
     CDS             3328..4032
                     /locus_tag="P5316_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86890.1"
                     /translation="MAETPDSEQPLQPASTWPRPIPRFALVLIGSLCTAVAVAILSKD
                     WCFSTVASTLATALIIGLLDSLVLERVIRDWSMGRRIQVILALLVCGSFLACFAGTGN
                     RLFTRVFGVSPPPGVSELAIDSASIGLSGDQAILMRFKADQATIERLVQHRGFVVDKE
                     TMTIWSQQMGKQWPWLWERAFSNFTIHGGRAWKNVEPMPDPVLFVHEHCEGIATITAK
                     MLWDRASGRTYLLYLN"
     gene            4164..4994
                     /locus_tag="P5316_17925"
     CDS             4164..4994
                     /locus_tag="P5316_17925"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRR86891.1"
                     /translation="MSDDAGNLSSAAECLRAAKNVVVFTGAGISAESGIPTFRDSDGL
                     WERFSPERFATWDGLVKTAVADPRLFTDFLIAVLEPIAVAAPNPAHLAVAELEKHVRV
                     IVVTQNVDGLHQEAGSTTVHEVHGSIFEIVTLGGRFVRLLSRTEIQGIVRKLREAREA
                     TLALPKALRAIHSMLGPSGVSIHRPKIVLFGEAMAEPAWSLAQEAAERCDCMITVGTS
                     AMVMPAAMLPGIARSAGASTIDVGLAPGEADFPLTGPAARVLPGLVRAAFGEPGGCGI
                     "
     gene            5691..7661
                     /locus_tag="P5316_17930"
     CDS             5691..7661
                     /locus_tag="P5316_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86892.1"
                     /translation="MNWATGNGVCLWALAALLVCALPAARASGQTGTLSLKGCTIQLV
                     EGQPAYVRRAAEDLGAVIQMFTGARPEVVQVRSGTEIVADRPVIAIGVGTATELIKGS
                     LFQKHADLGEAFVIHAERRDTSGSGVVPLVSIGSDHDPGGLKFGVFALIRRLKGSPGD
                     VGIDVPLTVEGRPHIRIRSMYAHLHWAYNNPYSLRTWSLQDWKRYIDLLAHLGFNCMQ
                     MWPMMELLPHPLSKEDEAYLRKYAEIIDYLHEQRGMKGFVVSCPNNITEDSRGVPIEN
                     REYFDFEKLLDPGEPDNLKKILDHRSDLYRTVPNADGYCVIDSDPGGWKGSPASAFVD
                     ILAGHRELIDKYGKRPSEQPLIYWTWMGWGTGTREQNIDQAVGGMVERVRPPWMMTVF
                     WPEHIAACEKYKLKDKAIHFPYGLIENEPCGPLTDLRIELIKREARWPIDHGLQAAQG
                     NAQTPLVQMPNIVAYSMVLWGEDPADPVDGAIPRLARGLVCRDASAVAEGWMSLTRED
                     VEDSRQLAERIRMLATDETARGPLALVIGEWQRRILEDLAFMLDIHAGGLEFARSAAG
                     DIENAALSRKLSAYLLSVGRWLERTGYHNVGMIAHKPYYDPVAKALTDLKGRMGDGEL
                     QAGIVQPATDVVRKQVDPKFVDPVARAICGKP"
     gene            complement(7665..8333)
                     /locus_tag="P5316_17935"
     CDS             complement(7665..8333)
                     /locus_tag="P5316_17935"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRR86893.1"
                     /translation="MSGCTPIDASGCLPQLPNPIINVREAPRVPPPPPPTSLKRISDR
                     TIVVDAGHGGKDPGALPKFRGQLREKDINLDIATRIGNDLSSRGARVVMTRTSDVFLE
                     LDDRSRLADRHRADLFLSIHADSSAKKWISGTGIHIHPRANYETMRMAQCIAAAFRRN
                     DISCRGIVRNNFHVLREHNSPGILIECGYMTNAADAQRLNDPAYRSRLASAIAEGIAD
                     YFSR"
     gene            8540..8674
                     /locus_tag="P5316_17940"
     CDS             8540..8674
                     /locus_tag="P5316_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86894.1"
                     /translation="MVLAATSVIEAAAVGSTTPAGELKSIGSSSLQINITTFVPLGGM
                     "
BASE COUNT         1635 a         2471 c         2954 g         1696 t
ORIGIN      
        1 atccgggcgg ccatcgagat gggcatggtg gacctgggcg agcggcgcgt gcaggacctg
       61 gtcaagcgtg ccgggatgat taacgagcac cttgcccggc gtcggacgtt ggagccgggc
      121 acatgcccgc cgacgcctcg ctggcacatg atcgggcatc ttcaaaggaa caaggttcgt
      181 cagttgctgc cgtgggtgga tatgatccac tcgctggaca gcctgcggct ggccgaagaa
      241 atcagtgatg aggcggcgaa gctcgggcgt gtggtcccct gtctgatcga gatcaacgtt
      301 tcgggcgaga agagcaagtt cggcattgcc gtgggggcgg ccccgtacct ggccgagcag
      361 gtggttgcca tgcccgggct gagtatcgtc ggcctgatga cgatggcgcc gctggtcgaa
      421 aatgcggaag aggcgcgatg ctatttccgt cggctgcgtg agctgttcga agacatgaaa
      481 cacgaaggcg tggtcaagcc ggagttccgg gagctttcca tgggcatgac caacgatttc
      541 gaggtcgcca tcgaggaagg ggcgactctg gtgcgtatcg ggacggccct gttcgaaggt
      601 ctgactaacc ccgtgcagca acagtagccg tctgtcgagc catcggtctt caaaagacgc
      661 cgcgggcgcg ctagaatccc atgttggttg gcccaccgct ttgggggcgg gggattgaat
      721 ccggtagaga atccgcgttt cgcacggccg aaaccgcagg accaccattc aggagcacgg
      781 aatgcccaat cgtccctgca aactagccct tgaggatggg accgtctttg ccggcgaggc
      841 ctttggtgcc gatggcacgc atgacggcga agtctgcttc aacacctcga tgaccgggta
      901 tcaggagatc atcacggatc cgtcgtactg cgggcagatc gtgaccatga cctacccgct
      961 catcggcaac tacgggatca ccgaagaaga tctcgaatcg cgcgggccgc aggttgaggg
     1021 tttcgtgatc aaggaactgg cccggcggca cagcaacttt cgcgcgggcg gctccctgga
     1081 agactggctt cgtaagcacg gggtcgtcgc gatcgagggc gtcgatacgc gggcaatcac
     1141 tcgtaagctg cggatccagg gggccctgcg cgggtcgatc tcaacagaga tactcgacga
     1201 cgcggccctg gttgagcggg cgcggagctg gcccgggctg gtcggtcagg acatggtcaa
     1261 acgcgtcgcg cccaaagaag ccttcaagtg gacgagcggc ttcgagagca agttcgcgat
     1321 ggatcaccgc cacaagacgg gcacgtatcg catcgtggcc atggactgcg ggatgaagcg
     1381 caatattctc cgcaatctgg tggaggccgg ttgcgaggtg acggttgtgc cgcccgacac
     1441 ggcagccgag gccattctcg aacacaagcc cgacggcatc ttcgtcagca acggcccggg
     1501 cgacccggaa ccgctggact acgcgatcaa gtcgctccgc ggactcgtcg gcaaggcccc
     1561 gatcttcggc atctgtctgg ggcaccagtt gctcggtctg gccctggggg ccaagacata
     1621 caagctcaag ttcgggcatc gcggcggcaa tcagccggtg atgaacctgg ccacacgccg
     1681 ggtcgagatc accagtcaga atcacggctt cgcggtcgag acgtcttcgc tggtcaaggt
     1741 cggcggcgtg cccacccatg agaacctcaa cgacaagacg ctggagggtt tctcgcacaa
     1801 ggacctgccg atcttctcgg tacagtatca ccccgaggcc agccccggcc cacacgatgc
     1861 tacctacctg ttcgactgct tcatcgacat gatccgcagc ggcaaggccc ctaccgccga
     1921 ggagatggcc gcggcacagg cgcggctgga aggcaaggcg gggtgagcgg agaaagcggt
     1981 cagcgatcag cggtcagctt tcagccggta gggcagagtt gagcctgcct cgccgcaggc
     2041 ggagtcgccc cggcgaccga gacctgccgc aagactgtgc cgtcttcgcc caccgatggg
     2101 ccgctccctt gacagctacg cagtcgggac catagccaca tgaggtgtgc atcggatata
     2161 atgatcttgt ggcaaggcaa catacttgcc gtccaaagga gaccgaacat gcgaacggtt
     2221 gaaacaacgg cgaagaccgg accggatggc gtgcttcgtc tagagttgcc gacggggcag
     2281 cctgaccaaa ccgtgcatgt cgtcgtcact cctcagccga tctccgaaac cgatcaacag
     2341 gcacgccttt tgaaggccgg catccgtccg ccggtgcgat ggaacgaaga gcagatcagg
     2401 cagctggacc tgggtgaccc gccggtttcc cagaccctcg ttgaggatcg gcgatgagcc
     2461 tctactacct tgatgccagc gcctgggtca agtgttacgt gcgggaagct ggaagcgact
     2521 ggatgcgtgg gttctgggac ctgcgcttgc ccgtggcgtg ttccagcttg gggtacatcg
     2581 aggtcatttc ggcgatcctt cgccgcgccg gacgctcgga ctcggagata ggcgcagtgt
     2641 tgggcaagat cggacgcgac cgcgaggcga tctacgaagc gggcgtttcc gcaaccgtcc
     2701 tcgagcaggc ggaggcgctt gtccgccgcc atcgcctccg cactgcggat gcaatccatt
     2761 tggcgtccgc cgccatgatg gggaaggaac tggaagagtc ggtcagcgtg attgcgtctg
     2821 atgccgagtt gcttgccgcc gcaaccgccg aaggaatggc agcgatcgat cctcaggcaa
     2881 acccccagtt gccgaccggc aagccgcagg catgaagcgc tactcccggc cacttcttcg
     2941 cgctggggtc aagcagaaac gcagaaagaa tcatcgtgtt gagccggcat cttgccgatc
     3001 cgcgacgggc tggaagcccg gcccgcacga tgctacctac ctgttcgact gcttcatcga
     3061 catgatccgc agcggcaagg cccctaccgc cgaggagatg gccgcggcac aggcgcggct
     3121 ggaaggcaag gcggggtgag cggagaaagc ggtcagcgat cagcggtcag ctttcagccg
     3181 gtagggcaga gttgagcctg cctcgccgca ggcggagtcg ccccggcgac cgagaccggc
     3241 cgcaagactg ccttgtcgcc gttgctcctc ggcgcgaacc cacttccgga catgagacac
     3301 gacggtatga tattccatgg agaacccatg gctgagactc ctgactctga gcagcccctg
     3361 caaccggcgt caacctggcc gcggccgatt ccgcgtttcg cacttgttct gattgggtcg
     3421 ttatgtactg cggtggccgt ggccattctg tcgaaggact ggtgcttctc cacggtcgcc
     3481 agcaccttgg cgaccgcact gatcatcggc cttctcgatt ctctcgttct tgaacgtgtc
     3541 atccgcgact ggtcaatggg acgcagaatt caggtgattc tcgccctctt ggtatgtggt
     3601 agcttcctgg cgtgcttcgc cggaaccggc aatcggctct tcacgcgtgt attcggcgtg
     3661 tcgccgcctc caggcgtaag cgagcttgcc atcgacagcg caagcatcgg tctcagcgga
     3721 gatcaagcta ttctcatgcg attcaaagct gatcaggcaa caatcgagcg gctcgtgcag
     3781 cacaggggct tcgtcgttga caaggagacg atgacaatct ggtcgcaaca gatgggaaaa
     3841 cagtggccct ggctctggga acgggcgttc tcaaacttca ccattcacgg cggccgagcg
     3901 tggaagaacg tcgagccgat gccagatccc gttctcttcg tccatgagca ttgcgaaggt
     3961 atcgcgacca taacggcgaa gatgctttgg gatcgtgcgt ccggaaggac ctaccttctg
     4021 tacctgaact gattgctcga tgtctaccag ggacggcggc aggcttcgtt tgccggttgc
     4081 catacccgta gcttcgcgtg ggcatcttct gcggtgcctc gcccaggcac cacacctgcg
     4141 gtatcatttt ctccagagaa cctatgagcg atgatgcagg caacctatcc tccgcggccg
     4201 agtgtcttcg ggccgcgaag aacgttgtgg tcttcacggg ggcggggatt tcggcggaga
     4261 gcgggattcc gacctttcgt gacagcgacg ggctgtggga gcggttttcg ccggagcggt
     4321 tcgccacttg ggacggtttg gtcaagacgg cggttgccga tccgcgcttg tttaccgatt
     4381 ttctgatcgc tgtgctggaa ccgatcgccg tggcggcacc taacccggcc cacctggccg
     4441 tcgccgagct ggagaaacat gttcgggtca tagtggtcac tcagaacgtc gatggcctgc
     4501 accaggaggc gggcagcacc actgtgcacg aggtgcacgg ctcgattttc gagatcgtga
     4561 cgctcggcgg ccgatttgtg cggctgctgt cgcggacgga aatccaggga attgtccgga
     4621 aactccgcga ggcaagggag gcaactttgg cgctgcccaa ggcgctgagg gccatccact
     4681 cgatgcttgg cccgagcggg gtctcgattc accggccgaa gattgtcttg tttggcgagg
     4741 cgatggcgga gcctgcctgg agcctggctc aggaggcggc cgagcgatgc gattgcatga
     4801 tcacggtcgg gacgtcggcg atggtgatgc cggcggcgat gcttcccggc atcgcgcgct
     4861 cggccggggc gtccactatc gatgtgggtc tggctcctgg ggaggcggat ttcccgctca
     4921 cgggcccggc cgcgagggtt ttgcctgggc tggttcgggc ggcgtttggc gagccggggg
     4981 gatgtgggat ctgagatttc agatttgaga gtttgagatg tgagagttga ggacttgaga
     5041 tctgaggttt gggattccgg attcgaggat ttgagatttc ggcgccgcct gaggtacgag
     5101 gaaccacacg ctgacgttcg ctggggtggt ttttgctgta aagcctttat tcgcagatac
     5161 ctagatgctt ttccagttct ttttccattt ttcctaggct acccagcttt gggtctgttt
     5221 ggcgcaaagg aatcttcagc gttcgtcagc ccgacaaggc cgagcaggag aagtctggcg
     5281 agtgcgattg cggggcgcga ggacacgagt tcgccgtttc accagacaac ccaagtttgg
     5341 gttggtttgg cgcggagaag ggatcagcga tctgcagtca gccggacggg gtagtgcaga
     5401 gtcactctgc atccggttga aggccgatcg cctccttcgg atggtttcac ttgtcaaaga
     5461 gcgcggtgtc gccatgtgct ctggcgcggt ggtaccacaa gaggtgttcc ggaaaggcgg
     5521 gcgcgaacgg agcggggtgc tggtctgttg atctattggt ccgttggttg gttcgtagaa
     5581 tcggtcgcca cggcggcctg ggcggtactg tatcgcccgg ctcggtcggc ggcgtgcttg
     5641 aacattgcgg ccacatgtgg gagaactcct gctttccaag gaggacagac atgaactggg
     5701 caacgggaaa cggggtttgc ctgtgggcct tagcggccct gctggtttgc gccctgccgg
     5761 cggcgcgggc gagcggacag accggcaccc tgtcgctgaa aggatgcacc attcaactgg
     5821 tggaaggcca gccggcgtac gtgcggcgtg cggcggagga cctcggtgcg gtgatccaga
     5881 tgttcaccgg cgccaggccg gaggtcgtcc aggtccggtc gggcacggag atcgttgccg
     5941 accggccggt catcgcgatt ggagtcggga cggccacaga gttgatcaaa ggctcgctct
     6001 tccagaaaca cgcggatctc ggcgaggcct tcgtcatcca cgcagaaagg agggacacgt
     6061 ccggctcggg cgtggtccca ctggtttcga tcgggtcgga ccatgatccg ggcgggctga
     6121 agttcggcgt tttcgcactg attcgccgct taaaggggag tccgggtgac gtcggcatcg
     6181 acgttccact gaccgtggag ggccggccgc acatccgcat ccgaagcatg tatgcccacc
     6241 tgcactgggc gtacaacaac ccgtactcgc tgcggacgtg gtcgctgcag gactggaagc
     6301 ggtacatcga cctgctggcc cacctggggt tcaactgcat gcagatgtgg ccgatgatgg
     6361 aacttctgcc gcaccctctg tcgaaggaag acgaggcgta cctgaggaaa tacgccgaga
     6421 tcatcgacta cctgcacgag cagcgaggga tgaagggttt tgtcgtgagt tgcccgaaca
     6481 atatcaccga ggactcgcgc ggcgtgccga ttgagaaccg cgagtacttc gatttcgaga
     6541 agttgctcga tccgggagag cctgacaacc tgaagaaaat actcgatcat cggagcgacc
     6601 tgtaccgcac ggtgcccaac gcggacggct actgcgtcat tgactctgac cccggcgggt
     6661 ggaagggcag tccggcctcg gcgtttgtgg atattctggc gggtcatcgt gagctgatcg
     6721 acaaatacgg gaaacgaccc agcgagcagc cgttgatcta ctggacgtgg atgggttggg
     6781 gcacgggcac gcgggagcag aatatcgacc aggctgtcgg cgggatggtc gagcgtgtcc
     6841 ggccgccgtg gatgatgacc gttttctggc ccgagcacat tgccgcctgc gagaagtaca
     6901 agctgaaaga caaggcgatt cactttccat atgggctgat tgagaacgaa ccctgcgggc
     6961 cgctgaccga cttgcggatc gagctgatca agcgggaggc tcgctggccg atcgatcatg
     7021 gtctacaggc cgcccaaggc aatgcccaga cgccgctggt gcagatgccg aatattgttg
     7081 cctactcgat ggttttgtgg ggcgaggatc cggccgatcc ggttgacggg gcgattccgc
     7141 gactggcccg cggcctggtt tgccgggatg ccagcgcggt ggccgaggga tggatgtcat
     7201 tgacgcgcga agacgtggag gattcgcggc aattggcaga gcggatccgg atgctggcca
     7261 ccgatgaaac ggcccgaggc ccgctggctt tggtcattgg cgagtggcag cgtcgcattc
     7321 tggaagacct ggcattcatg ctcgatatac acgcaggagg gctggaattc gcccgttcgg
     7381 cggcaggtga catcgagaac gccgcgttga gcaggaagtt gtccgcgtat ctgctgtcgg
     7441 ttgggcgatg gttggagcgg accggttacc acaatgttgg gatgatcgcc cacaagccct
     7501 attacgatcc tgttgccaag gcgctgaccg acctgaaagg tcggatgggc gacggggagc
     7561 tgcaggccgg aatcgtgcag ccggcgaccg acgtcgtgcg caagcaagtc gatccgaaat
     7621 ttgtcgaccc ggtggcgagg gcgatctgcg gcaaaccata gaggtcagcg ggagaagtag
     7681 tcggcgatgc cctcggcgat ggcggaggcg agcctggaac ggtaagcggg gtcgttaagc
     7741 cgttgagcat cggcggcatt ggtcatatac ccacattcga tgaggatgcc ggggctgttg
     7801 tgttcgcgga gtacgtggaa gttgtttcgc acgattccac ggcaggatat gtcgttccgg
     7861 cgaaaggccg ccgcgatgca ttgggccatt cgcatggttt cgtagttagc gcgggggtga
     7921 atgtggatgc ccgttccgga gatccatttc ttggctgacg agtcggcgtg gatcgacaaa
     7981 aacagatcgg cgcggtgtcg gtcggccagc cttgaccgat cgtcgagttc aaggaacaca
     8041 tcactggtgc gggtcatcac cacccgggcc ccccgagagc tgaggtcgtt gccgattctg
     8101 gtagcgatat ccagattgat atctttctct ctcagttggc cgcggaactt gggaagggca
     8161 ccgggatcct tacctccatg gccggcgtcc acgacgatgg tccgatcgct gattctcttg
     8221 agtgatgtgg gaggaggtgg cggggggacg cgcggcgcct cgcggacgtt gatgatcggg
     8281 ttggggagct gaggaagaca gccggaagca tcaatcggcg tgcaaccgga caaagacaga
     8341 cccgttatga gtactgacag ggctgcgagg atcggccaat ggggcgcacg agccggttga
     8401 tcagataggg ggctgcatga cggggtgcga ccgccggcgg tttcgggggg cagaatgcgt
     8461 ccgctgtggg ccatacgcgt ccgtgctctc agccttgcga cgccggcgtt ccgtctcgga
     8521 tcaaaggcgt ctgcgattca tggttcttgc tgcaacttcc gtgatcgagg cggccgccgt
     8581 tggcagcacc accccggcag gcgagctgaa gagcatcggt tcttcaagcc tgcagatcaa
     8641 tattacgacc ttcgtcccct tggggggaat gtgactggtc cgcgttctgt tcttgttcct
     8701 ctggcgaact gaaggcccta ccctgactac cggtgccctc ggcgtccgcg tggatc
//
LOCUS       DAOTPW010000245         8738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7296_length_8738_cov_6.312104, whole genome shotgun sequence.
ACCESSION   DAOTPW010000245 DAOTPW010000000
VERSION     DAOTPW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7296_length_8738_cov_6.312104"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="P5316_17945"
     CDS             complement(<1..349)
                     /locus_tag="P5316_17945"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR86895.1"
                     /translation="MIRTLTAFLSILVILSSGCSTGRRLFNGKDTSGWVEVGSQGAWT
                     AETGNVCPTRGPALKCSGRNIGYAWLSTDREYGDFELTLEWRINPGGNTGVFCRVPDR
                     DGRASMKGFEVQAR"
     gene            complement(430..1521)
                     /gene="rsmH"
                     /locus_tag="P5316_17950"
     CDS             complement(430..1521)
                     /gene="rsmH"
                     /locus_tag="P5316_17950"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRR86896.1"
                     /translation="MPSNEPGKRPHPYRRRARYSGTHPRRFEDRYKELNPQDYPGIHE
                     HVRGRGHTPAGTHVPVMVDEVMACLRPMPGEVVADCTVGFGGHAREFLKRIGPNGRLL
                     AFDVDERELERTRQRLADAGFVNNVRFHRSNFAGIGKTLMMEQLDGYDVIFADLGVSS
                     MQVDDPSRGFSYKHDGPLDMRMDQRIPRSAADWLSDLSEEELVAILRDLADEPDAEPI
                     ARQIIECGTVEPIKRTGQLVQIIFKAKRLTRRRWRERAARDQRELHPAARVFQALRIL
                     VNDELSSLRHLLRIAPSCLRPGGRIGIISFHNGEDRLVARSFREGLQSGAYASAADEA
                     IRPGRQERYDNPRCASAMLRWAQTPHVRS"
     gene            1911..2705
                     /locus_tag="P5316_17955"
     CDS             1911..2705
                     /locus_tag="P5316_17955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR86897.1"
                     /translation="MLLADSFFYYPNRTEYERPCDYGLKFEDVSIGTPDGLRLHGWFF
                     PAESEPAGTVLHLHGNAGNLTGHFQHVAWLPAAGWNVLCFDYRGYGRSEGRISRRGSI
                     ADANAALDWLLAQPKVDRGRIVAFGQSLGGAIGIVLTAQRPEIRGLIADGAFDHYRRI
                     ARWHILRNPVLLVLAWWLPLLMSRDLNPIDFVSRIAPRPILLMHGTADTVCPAVMARR
                     LYAAAAEPKEIWLIEGANHYQAMQEMPEQTHPRMLAFFEKCVRRQP"
     gene            2960..3622
                     /locus_tag="P5316_17960"
     CDS             2960..3622
                     /locus_tag="P5316_17960"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRR86898.1"
                     /translation="MSNSEMLAGLASAVENLDAKNVKSLVEQALASGMKPADILTNGL
                     SEGMRRVGKAFNTGDMFMPEVLVACDVYFAGLAVVRPLLLAGGDDCVIGTMVMGNIHG
                     DIHTVGKNVAMPVFEANGIRVIDIGVSASDDAFVEAIRQHKPQIVGFGTYMTSTFMHT
                     KETVAAIEKAGLRNQVKIICGGPAVDADAARRMGADDASDDAWVAVKKIKRLLGAPAD
                     EE"
     gene            3711..4874
                     /locus_tag="P5316_17965"
     CDS             3711..4874
                     /locus_tag="P5316_17965"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR86899.1"
                     /translation="MSKVVDQWVERLKGKIKPVGEMTHWERMAAAFDLKETDMVPVAP
                     ELDYWQITYAGYSHKAIYNDVDKTTDACIKTWADLRCDAIWMYVDISHQIENFIPPEK
                     RPEHFIHRGKDDYLLFKPIAQTLDEAIAMYEQRTWEKYTAGSRPATHYTPHMLQLLEF
                     QEKMGRQVPVIVGIATPTNYAETVVEVQNFLKWMVTEPEDKIRYYLDLCLKERLEALD
                     FYAEFAAKNGAQFCCGFGGARTWGPRHLDKFGDVDRIWLDKAKKIFPYVFWHICGHNL
                     PKALELLAAWDGIKAVQYDMPYYGQDISWPQWCEQVARLFHGKRCAMNAPTTQMACHG
                     TADQVSRMVAEFIDATLPHTTACVMPGCEIDSFSPVANVRAMIDTARSRRGSA"
     gene            4871..5551
                     /locus_tag="P5316_17970"
     CDS             4871..5551
                     /locus_tag="P5316_17970"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRR86900.1"
                     /translation="MSIQQQVIDLEITLDPEEVLRTMGGVRKGPIKQSIVRMVHEYVG
                     RFQDDIRPRGVYAVCDVMSIGEGRLHLRDGHVFEGQIADFLEPAGRVAVFIVTIGEEI
                     EQAAERRMEQGATLEGFTIDAIGSVAADSAADAMADHVLWCDAQAGEAVTLPFSPGYC
                     GMALEEQKTLFSILDSNLVGVELLPTMIMRPIKSVSGLVGIGPQDRIAAHGIPCERCR
                     VKSCRMRR"
     gene            complement(5775..5975)
                     /locus_tag="P5316_17975"
     CDS             complement(5775..5975)
                     /locus_tag="P5316_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86901.1"
                     /translation="MDKAKRSYFLPSKLVTLFDRECTKGGYVRERVVAAAIANFLKAS
                     PVERHEMFVYLDQLMTGGKGKK"
     gene            complement(6233..7600)
                     /locus_tag="P5316_17980"
     CDS             complement(6233..7600)
                     /locus_tag="P5316_17980"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRR86902.1"
                     /translation="MGLFGLILVHMLTAVPPADSATSEADAKMPPASQPAIIRGLMFV
                     GGHAHDFDMEPARLAELLRRQENIDIRITTDLGELTTGNVATVDVLMFNACLDKGMDE
                     AKQRVLLDALRRGKGLVAMHCALWCFQDWVEWRRMLGGIVLKHDLYGPFEIGVVYPAH
                     PIAQGVPATLAITDEPYYVDERGPDICVIAQTLRTHPGRQTSEPQVWTNRYMGGRIFV
                     IAMGHDEHALFHPAYLKLLTNGIRWAATRLGPAQVLSDHERREGFIPLFDGTTLRGWR
                     YDPRLWTVRDGTIVGSTYPTPLAVNSCAIADGRYSDFILRFSVKFVSGNSGVQFRSLE
                     LPNFEVAGYQVDIVPLGWGNLHEQNGRRRLVDGWTKKAEYAVNLQDWNDMEVQARGRR
                     IILKTNGVITADYLETDESVPLTGIIALQLHRETLMEVQFTNIRIKPLDTSDASGKAE
                     SPLRR"
     gene            complement(7627..>8738)
                     /locus_tag="P5316_17985"
     CDS             complement(7627..>8738)
                     /locus_tag="P5316_17985"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRR86903.1"
                     /translation="ETPWTAVSAERDFTHIFQLTGLKTATVYHYMVETAPPDTDSVSA
                     GLRGLFETLPRPDQETDMCFTVMTCQMFCRRDDPEGFLIYDAMAALKPKFTLFTGDSV
                     YLDNEPPIANTVELARHHWHRMHSLPRLVRFYLQVPGYWEKDDHDTYCNDCWPTIEAP
                     LMAPLTYEQGLRVFREQVPAPPRLYRTVRWGKDLEIWLVEGRDFRSPNDMPDGPDKTI
                     WGIEQKEWLKKTLVASDATFKILVSPTPIIGPDRAKKADNHSNKAFAHEGNEFRQWVR
                     ENLPGSFMIFCGDRHWQYHSVDPATGVEEFDCGPASDEHAGGSPGLDPAYHRFHRLKG
                     GFLSATVQRIDDHPRLIVRLHDVGGRVVYERRFEQ"
BASE COUNT         1684 a         2497 c         2744 g         1813 t
ORIGIN      
        1 cgcgggcctg gacctcaaag cccttcatgc tggcgcggcc gtcgcgatcg gggactcggc
       61 agaacacgcc ggtgttgccc ccggggttga tccgccactc gagggtcaac tcgaagtctc
      121 cgtattctcg gtcggtgctc agccaggcgt agccgatgtt ccggccgctg catttgaggg
      181 ccggacctcg cgtggggcac acgtttccgg tctcggccgt ccaagcacct tggctgccga
      241 cctcaaccca tccggaggtg tctttgccgt tgaacagccg tcttccggta ctgcagccgg
      301 aagacaagat gacgaggatc gacaagaacg cggtcagcgt acggatcatc ttgcactcct
      361 tcaggaagct gccagctcct gactgtttgc cgtttcgttt tgagtggacg ggattataca
      421 aaggcggttc tatgaacgca cgtgcggggt ctgggcccat cggagcatgg ccgaggcgca
      481 acggggattg tcgtaccgtt cctgacgtcc cgggcggatg gcctcatcag cggccgacgc
      541 atacgcgccg gactgtaatc cctcgcggaa tgatctggca acgagtcggt cctcgccatt
      601 gtggaagctg atgatgccaa tgcgaccgcc tggccgcaga caggaaggcg cgatgcgaag
      661 caggtgccgc aggctgctca gctcgtcgtt gaccaggatc cgcagggcct ggaagacccg
      721 cgcggccgga tgaagctcgc gctggtccct ggccgcacgt tcgcgccacc gtcggcgggt
      781 aagccgcttc gccttgaaga tgatctggac cagttggcct gttcgcttga ttggttcgac
      841 tgtcccgcac tcgatgattt gccgggcgat tggttcggca tcgggttcgt cggccagatc
      901 gcgcagtatg gcaaccagct cttcctcgga gagatcgctt aaccagtcgg cggcggagcg
      961 cgggatgcgc tggtccatcc gcatgtcgag cggcccgtcg tgcttatagc tgaagccccg
     1021 ggacgggtcg tcgacctgca tgctggagac gcccagatcc gcaaagatca cgtcgtagcc
     1081 gtcgagttgt tccatcatca gggtcttgcc gatgccggcg aaattgctgc ggtgaaagcg
     1141 gacgttgttc acgaagccgg cgtccgcgag acgctgccgg gttcgctcga gttcccgctc
     1201 gtccacgtcg aacgccagca gccggccgtt cggcccgatc cgcttgagga actccctcgc
     1261 gtggccgccg aagccgaccg tgcagtcggc gacgacctcc cccggcattg gtcgcaggca
     1321 ggccatgact tcgtcgacca tgaccgggac gtgcgttccg gccggcgtgt gcccccgacc
     1381 ccgcacatgc tcgtggattc ctggatagtc ctgcgggttc agttccttgt agcgatcctc
     1441 gaaccggcgg ggatgagtgc cggaatagcg cgctcgccga cgataaggat gcggcctctt
     1501 gccgggttcg ttgcttggca tcgtttgcta acgggcttcg ttgacatcat gtcgcctgtg
     1561 atcgcaccac tctgttttca tgacagaatc agtctgctcc gcacaggaac gcagacgccg
     1621 ctttgcggag caccaggcga taaagcgact tcgaccatag gagtatccta gcaggaagct
     1681 gaccggtcgg cgagccaccg gaccggcatg cgagacctcg agtccgggat gctccgggcg
     1741 gttctttgag aatcccgagc gtcggatcaa cggcgaattc gggcttcgcg gcgtccggtg
     1801 caatccggct ggttggacag caccatcgtg gttaggatac ccgtttggtg agcacgggca
     1861 cgagcctttg cttctggatc cggaggaacc gatagctgat cttcagtctc atgcttctgg
     1921 ccgatagctt cttttactat ccgaaccgca ccgagtacga gcggccttgc gattacggtc
     1981 tgaagttcga ggacgtgtct atcggcacgc ctgacgggct gcggcttcat gggtggttct
     2041 ttccggcaga gagcgagccg gcgggcaccg tcctgcacct tcacggcaac gcgggcaatc
     2101 tcaccggcca ttttcagcat gttgcatggc tgccggcggc cggctggaac gtgttgtgct
     2161 ttgactatcg cgggtacgga cgttcggagg ggcgcatcag ccgtcggggc tcgatcgccg
     2221 atgcgaacgc cgcgttggac tggctgctgg cacagccgaa ggtcgatcgg ggccggatcg
     2281 tcgcgtttgg gcaatcgctc ggcggggcga tcgggatcgt cctgacggcc cagcggccgg
     2341 agattcgcgg cttgatcgcc gacggggctt tcgatcacta ccgacgcatc gcccgttggc
     2401 acattctgcg taacccggta ttgctggtat tggcgtggtg gttgcccctg ctgatgagcc
     2461 gcgatctcaa tcccatcgac ttcgtctcgc gcattgcccc ccggccgatc ctgctgatgc
     2521 acggtacggc cgataccgtc tgcccggcgg tcatggcccg ccggctgtac gccgccgccg
     2581 cggaaccgaa agagatctgg ctcatcgaag gagccaacca ttaccaggcc atgcaggaga
     2641 tgcctgagca aacgcatccg agaatgctgg cgttctttga gaagtgcgtg aggcggcaac
     2701 cgtagggttc caggcttcgc cagaccgtga cgagaggggc ggatcagacg gcaggtcttt
     2761 gcttcgaccc actcaaaaaa aggctttgag acgcgtgtga ggcaaggcaa acaacagatg
     2821 atcatggaca tgagaagcgg cgggcgatat tctaacacgg tcccaggcgg atgtttcgcc
     2881 ggaggggaat ccgtgcacgg gacggcagca acggtactgt gggtgttgtg acgagcatgc
     2941 gaccggaggt atggcactga tgagcaactc ggagatgttg gccggcctgg cctcggcagt
     3001 ggagaacctg gacgcgaaaa acgtcaagtc cctggtggaa caggctttgg cgtcgggcat
     3061 gaagcctgcc gacatcctga cgaatggtct gtcggagggc atgcgtcggg tgggcaaggc
     3121 tttcaatacc ggggacatgt tcatgcccga ggtgctggtg gcgtgcgacg tatacttcgc
     3181 gggcctggcc gtggtgcgcc cgttattgtt ggccggcgga gacgattgcg tgatcggcac
     3241 catggtgatg ggcaacatcc acggtgacat ccacacggtg ggcaagaacg tggccatgcc
     3301 tgttttcgaa gccaacggca tccgggtgat cgacatcggc gtcagtgcca gcgatgacgc
     3361 cttcgtcgag gcgatccgcc agcacaagcc ccagattgtc gggttcggaa cgtacatgac
     3421 ctcgactttt atgcacacca aggagacggt cgcggccatc gaaaaggccg gtttgcggaa
     3481 tcaggtcaag atcatatgcg gcggtccggc ggtggatgcc gatgccgctc gcagaatggg
     3541 agccgatgac gcgagcgacg acgcctgggt cgcggtcaag aaaatcaagc gtctcctcgg
     3601 cgcgccggcc gatgaggaat gaggttttcc ggtcgtgcga gcggccggca tcgccgcatg
     3661 cggacaggaa ttcgaggcgt caagacggtt ttcgacacgg agccaggggc atgagcaagg
     3721 ttgtcgatca atgggtggag cgtctgaaag gcaagatcaa accggtgggc gagatgactc
     3781 actgggagcg aatggcggcg gctttcgatc tgaaggaaac cgacatggtt cctgtcgctc
     3841 cggaattgga ctactggcag atcacgtatg caggctattc gcataaggcg atctacaacg
     3901 acgttgataa gacgactgac gcgtgcatta agacctgggc cgacctgcgc tgtgatgcga
     3961 tctggatgta cgtggacatc agccaccaga ttgagaactt tatccccccg gagaagcggc
     4021 ccgagcattt catccatcgt ggcaaagacg attacctgct tttcaagccg atcgcccaaa
     4081 cgctggacga ggcaatcgcc atgtacgagc agcggacttg ggagaaatac acggcaggtt
     4141 cccgccccgc cacgcattat accccgcaca tgcttcagct tctcgagttc caggagaaga
     4201 tgggccggca ggtgcccgtg atcgtgggca ttgccacgcc cacgaactat gccgaaacgg
     4261 tggtcgaggt ccagaacttc ctgaagtgga tggttaccga gccggaggac aagatccggt
     4321 attatctgga cctctgcttg aaggaacgtc tcgaggcgct ggatttctat gccgaattcg
     4381 ctgccaagaa cggggcccag ttctgttgtg gattcggggg cgcgaggacc tggggacccc
     4441 ggcatctgga caagttcggc gacgtcgacc ggatctggct ggacaaggcc aagaagatat
     4501 ttccttatgt cttctggcat atctgcggtc acaacctgcc taaggccctg gagctgctcg
     4561 ccgcttggga cgggatcaag gcggtgcagt acgacatgcc ctactacggt caggatatat
     4621 catggccgca gtggtgcgaa caagtggcca ggcttttcca cggcaagcga tgcgccatga
     4681 acgctccaac aacgcagatg gcctgccacg gcaccgccga ccaagtcagc cgtatggtcg
     4741 cggagttcat tgacgcgaca cttccgcaca ccacggcgtg cgtcatgccg ggttgcgaaa
     4801 tagactcttt ctcgccggtt gcgaacgtga gggctatgat tgatacggcc cgatcccggc
     4861 gagggtccgc atgagcattc aacaacaagt catcgatctg gagattaccc tggatccgga
     4921 agaggtgctg cgcacgatgg gcggagtgcg caagggcccg atcaagcagt ccatcgtgcg
     4981 tatggttcat gagtatgtgg ggcggttcca ggatgacatt cgtcctcgcg gggtgtatgc
     5041 cgtttgtgat gttatgtcca tcggcgaggg ccgtttgcac ttgagagacg gtcatgtttt
     5101 cgagggacag atcgcagatt ttcttgagcc ggccggtcgc gtggctgttt tcattgtgac
     5161 cattggcgag gagatcgaac aggcagccga gcgtagaatg gagcagggcg cgacgctgga
     5221 gggcttcacc attgacgcca tcggctccgt ggctgccgac tcggcggccg atgccatggc
     5281 agatcatgtt ctctggtgcg atgcccaggc gggggaggcg gtaacgctgc cgttcagccc
     5341 gggttactgc gggatggccc tggaagagca gaagacgctg ttttcaattc tcgattcgaa
     5401 cctggtgggc gttgaactgc tgccgaccat gatcatgcgt ccgataaaga gcgtgtcggg
     5461 ccttgtcggg attggtcctc aagaccgaat tgccgctcat gggattccct gtgaacgctg
     5521 tcgagtcaaa tcgtgcagga tgcgccgata ggttcggcga cttgatcttc ttgtaccctt
     5581 tcggccaagt acatggggag caagagaccg gttgttaccc tgcggcgccc gaatctagcg
     5641 cacacttcgg tgaaccgaat cactgaaagc ggccggcctg ataggatggt attgaacaga
     5701 ggccgcgcgg catcgatccc cgaaggcacc tccggcaccc cagaagcagg tgccggaggt
     5761 acgtcggtcg gcgtttactt cttgcccttg ccgccagtca tgagctggtc gaggtagacg
     5821 aacatctcat gccgctcaac cggcgaagct ttgaggaagt tggcgatcgc ggccgccacc
     5881 acgcgttccc gaacatagcc gccctttgtg cactcgcgat caaacagggt caccagcttg
     5941 ctcggcagaa agtaggatcg ttttgcctta tccatgtgtt actcctttca gacgtaaaac
     6001 gacgttcggt attatgagca aaagcaaaga aaaagcaagc ctcttttctg ggattcgtgg
     6061 cggttttggt gtccttttgt tccacgcggg acaaatgcct ctgtctttgc cggcgtcaat
     6121 ccgccacgtt cagaagaacg gcgcttgtca gcccggttcg atcgctagtt ggatgcgaca
     6181 gcatcttgtg ccgcctgatc gatagccagc aatgtgaggc tttcatcttc acttaacgcc
     6241 ggaggggcga ttccgccttc ccggaggcgt ctgacgtgtc gagaggcttg atccggatat
     6301 tggtgaactg aacttccatc agcgtctcgc gatgcaactg caaggcgatg attccagtga
     6361 gaggaacgct ttcatcggtt tccaggtaat cagcggtaat caccccattg gttttgagga
     6421 tgatgcgccg gccgcgggct tggacttcca tgtcgttcca atcttgcaga ttgacggcgt
     6481 attcagcctt tttggtccag ccgtcaacaa gtcgtctgcg gccgttttgt tcgtgaaggt
     6541 ttccccagcc caacggaacg atatctactt gatatccagc aacttcgaag ttcgggagtt
     6601 cgaggcttcg aaattgaacg ccgctgttgc cgctgacaaa cttgacgctg aaacgcagga
     6661 tgaaatcgct gtagcgaccg tcggcgatcg cgcaggagtt gacggccagg ggtgtggggt
     6721 aagtgctccc gacgatcgtt ccatcgcgga ccgtccacag tcttgggtcg taacgccatc
     6781 cgcgcagcgt tgttccgtcg aacaacggaa tgaaaccttc ccggcgctca tgatcactga
     6841 gcacctgcgc gggtccgagt cgggtggctg cccaacgaat tccgttcgtc agcagcttca
     6901 gataagccgg atggaataaa gcatgttcgt catgccccat ggcaatgacg aatattcgcc
     6961 cacccatata gcggttcgtc cacacttgcg gctcggaagt ctgtcggccc ggatgggttc
     7021 ttaaggtctg ggcaatgacg cagatatccg gtccgcgctc atccacatag tatggttcat
     7081 ccgtaatcgc aagagtggcc ggcacccctt gggcaatggg atgtgcgggg taaaccaccc
     7141 caatttcaaa gggcccgtac aggtcgtgct tgaggactat gccgcccagc atccggcgcc
     7201 attccaccca atcctggaag caccagagag cacagtgcat ggcaacgagg cctttgcccc
     7261 tgcgaagcgc gtcgaggagt acgcgttgtt tcgcctcgtc cattcccttg tcgagacagg
     7321 cgttgaacat cagcacatca acggtcgcca cgttgccggt cgtcaactcg cccaagtcgg
     7381 tggtgattcg aatgtcgatg ttctcctgtc gcctgaggag ttcggctaat cgtgccggtt
     7441 ccatgtcgaa atcatgggca tggcctccga caaacatgag gccgcgaatg attgccggtt
     7501 gtgacgccgg tggcattttt gcatccgctt ccgatgtcgc cgaatctgcc ggcggcacgg
     7561 ccgtcagcat gtgaacgaga atcaggccga aaagtcccat gatcgatgct cccaactacc
     7621 gttatctcac tgttcgaatc gtctttcgta caccactcgg cccccgacgt cgtgcaggcg
     7681 cactataagg cgcggatggt cgtcgattct ctgcacagtg gcggacagga agccgccctt
     7741 gaggcgatgg aaccggtgat aggcagggtc cagaccgggt gaaccgccgg catgctcatc
     7801 gctagccggc ccacagtcga attcctccac ccctgtcgcc ggatccaccg agtggtactg
     7861 ccagtgtcga tcgccacaga agatcatgaa gctccctggc aggttttccc tgacccattg
     7921 gcgaaactcg ttgccctcgt gagcgaacgc cttgttggag tggttgtcgg ccttcttggc
     7981 tcggtccgga ccgatgatcg gcgtcgggct gacgagaatc ttgaaggttg catcactggc
     8041 cacgagagtt ttcttcagcc actccttctg ttcgattccc cagatggtct tgtcggggcc
     8101 gtcgggcata tcgttgggag atcggaagtc gcggccttcg accagccaga tttcgaggtc
     8161 tttgccccac cgcacggtgc gatacaaccg cggaggcgca ggcacctgct ctcgaaagac
     8221 gcgcagcccc tgctcatagg tcagcggcgc catcagcggg gcttctatcg tcggccagca
     8281 gtcgttgcag tatgtgtcgt ggtcgtcctt ctcccaatag ccggggacct gcaggtaaaa
     8341 ccggaccaag cgaggcaggc tatgcattcg gtgccagtgg tggcgtgcga gttcaaccgt
     8401 gttggcaatc ggtggctcgt tgtcaagata gacgctgtcg ccggtgaaca aggtgaactt
     8461 gggtttgagc gcggccatcg catcgtagat gagaaacccc tccggatcat cgcgcctgca
     8521 gaacatctgg caggtcatga ccgtgaagca catgtcggtc tcttgatcgg gccgaggcag
     8581 ggtctcgaag agacctcgca aaccggctga aaccgagtcg gtatcgggcg gtgcggtctc
     8641 gaccatgtaa tgataaaccg tcgcagtctt gagaccggtg agctggaaga tgtgcgtgaa
     8701 gtctctttcc gcggacaccg ccgtccacgg tgtctccg
//
LOCUS       DAOTPW010000246         8659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7370_length_8659_cov_5.476174, whole genome shotgun sequence.
ACCESSION   DAOTPW010000246 DAOTPW010000000
VERSION     DAOTPW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7370_length_8659_cov_5.476174"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            804..1715
                     /locus_tag="P5316_17990"
     CDS             804..1715
                     /locus_tag="P5316_17990"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86904.1"
                     /translation="MFLEKLKPCTITVVTAILIQTLFMAAPVSADVYGIKNRSASGDG
                     TKEYEPPSILFHFADRGGSPTMVGYITDGDGTQLRADGLAWSVSLGMWFFEPSAPGEP
                     VSSTLRSLNPDTAVAGAGVTLPGRFIFGAAFDTSNRLWATDEASDELLRIDPNTGSVL
                     YSVGLIYEGDTFNLHNQSGDIAFDALGNAWLVFEDAFYRVDTATGVMTYDRTVSGISL
                     YMVGAAFSPVDVDDLYVFDVTAGWNNDDILRFDLGNDYARTDVALYIFNDMNAGRGDL
                     ASPMVPEPASLTILTLGGLALFRRRKG"
     gene            complement(2133..3542)
                     /locus_tag="P5316_17995"
     CDS             complement(2133..3542)
                     /locus_tag="P5316_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRR86905.1"
                     /translation="MKSRDLIKMGYPAGPILHLALSAAGEARVSGQRKSKVLQTLRML
                     LIDAHPYVEDPLFGKIAKALIAAGLVAQPKPTAADYKLNESLEYKVWGDDIDEQAHQQ
                     MRNACKLPISVAGALMPDAHVGYGLPIGGVLATDNAVIPYAVGVDIACRMMMTVFDLP
                     PHLLQDQDDRFKAIIENNTRFGIGAEWKPKREHPVMDEDWNISQVTARLKDKAHRQLG
                     TSGSGNHFVEFGEVTFLESVRGVSPGKYLAVLSHSGSRGPGNEAAGFYSKWAMSRHKS
                     MPKELRHLAWLELDGPGDEYWAVMELMGRYASANHHIIHRQIVKAVGEPPLLQIENHH
                     NFAWKEQHGGRTVIVHRKGATPAGKGVLGIIPGSMASPAYVVEGLGNEESLQSASHGA
                     GRLMSRGEAIRTFKWPQVRQFLKEKGVTLLSAGLDEAPWVYKDIEKVMAAQETLVRPV
                     ARFMPRLVKMSPPGDRPED"
     gene            complement(3552..4613)
                     /gene="queA"
                     /locus_tag="P5316_18000"
     CDS             complement(3552..4613)
                     /gene="queA"
                     /locus_tag="P5316_18000"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRR86906.1"
                     /translation="MRTSELQYELPTELIAQEPPEQRDASRLLVLDRSTGTLRHEVFA
                     RLPHLLPPKALLVMNDTRVLPARLYMARRSGGRVEGLFLRETEAGAWEIMLTASGRLK
                     TGEELRINGSTRRLRLTRSAKAGVWFAEPVPCAPAVEILAECGHPPLPPYIRRGTQDN
                     STAGKDERERLDRDRYQTVYARRPGAVAAPTAGLHFTPAVLNELRTAGIETSFVTLHV
                     GVGTFAPIRCDDLAEHEMHAEWYDCPAATAEAVNAAQAQDRPIVAIGTTSARVLETCA
                     DESGRVTMASGWTRLFIYPPYRFKVVEGMVTNFHLPGSTLLAMLFAFAGREAILNAYN
                     EAIRERYRFYSYGDAMLVL"
     gene            complement(5113..5352)
                     /locus_tag="P5316_18005"
     CDS             complement(5113..5352)
                     /locus_tag="P5316_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRR86907.1"
                     /translation="MDYQQFIVRDPRVCGGQPVIRGTRVTLQTVLASLADGDGIDEIL
                     ADLPTLTRSQVEAVIAFAAASASEDLPTPGVPHLT"
     gene            complement(5526..6914)
                     /locus_tag="P5316_18010"
     CDS             complement(5526..6914)
                     /locus_tag="P5316_18010"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86908.1"
                     /translation="MQSGSKSGNATDAVSRRVFLSGTMGAAAGLAAPYVIGSRAGAAE
                     TRPAAARVSPNDKIRIGLIGANGQGKFSLDQLMQQPDAVITAVCEVFEKRRDEALAKC
                     NGTAKPYGDYRKVLENKDIDAVVIATPPHWHALMAIEAAEAGKDFYLEKPMTLTVGEC
                     LAVHHAVQKYKRITQVGTQVHATANYRRIVDVVRSGILGPISMVRVFHVLNQGPEGIG
                     RTPPTPVPQGLDWEMWLGPGPKRPFHPLLFKNSEFHPSFMAYSGGWTPGMAPHLLDLP
                     YWALDLGHPTCTSSTGGRYIIDDDGDAYDFQQIQWQFPKLTVCWWTSLVNSFGFDTQG
                     QPGVHRRRGIYFHGTNGTLIADYGYLKIVPEGDRMEEEDVEKVEKVTPDSPGHHREWL
                     DGIRTRTPPSCHVGYHYKVDMAINLGMLSLKLGRSVQFDPVTETIPNDPEAQKHVNPV
                     YREPWRFPAQYV"
     gene            complement(7229..8158)
                     /locus_tag="P5316_18015"
     CDS             complement(7229..8158)
                     /locus_tag="P5316_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR86909.1"
                     /translation="MSIEQSIDRRDFVKLVGAGLMAVGGGHLAAGAENTAVPAAKPAA
                     KFKKAIGFEMVGEDLSVLDKFKLLKELGFDGVEVQSSGRPDREEFLKARDATGLEIHS
                     VMNPDHWKKPLSHPDPKVREECRKSMQGSLEDAKYWGAGSVLLVPAVVSKEIAYDEAY
                     TRSQAEIRKLAPIAEKLGVVIGIEFVWNNFLLSPLEFARYIDEINSPMVQAYFDCGNV
                     VRYGWPEQWVRILGKRICKIHIKEYSRQKANEEGVWAGFDVELMEGDCDWPSVTKALR
                     EVGYSSYITAEVKGGKKARLAQLAELIDKVIAG"
     gene            complement(8375..>8659)
                     /locus_tag="P5316_18020"
     CDS             complement(8375..>8659)
                     /locus_tag="P5316_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86910.1"
                     /translation="LTESQVPMGDLPATIPPGEHRTFGVIASPDTIARTRMGELIVRS
                     NWGDVVVSLKAYGVGIDRTPGGCGTIGPVSGIAMLAGFALLSICGHRRRD"
BASE COUNT         1665 a         2691 c         2577 g         1726 t
ORIGIN      
        1 agcagcttcg tctttgactt tcaactgctt cccgcaggac gtgcatgcaa attcgatcat
       61 gccatgcctc ccaaatcaga acgttttgtc gccattatcc accgtacctg ctgcactgca
      121 agtctaaacc tgacatgctc ttcttgatgg ttgcgttcgc accccaccgc catttcgaac
      181 ttgcgccggg taattgatta acaggcagac atcgtaaacc agccagacag cgagccggtc
      241 gccaggcgaa ggccgttgtg ggcgcatccg caaggagccc gcaatggaag aaccgttcgc
      301 cgaccccgcg agacgtgagc acgagcgccg ccaggagatc gccgccgtac tcgccggacc
      361 tgatgccgat cgagaatgcg ttttcgaaaa tcaagcagga gctatgacgt gtgcgacacc
      421 gtacgcgaga agcgatgtgg caagacacgc cgcgactgat gtcgctgatc acgccaaaag
      481 acgcgagaaa ctttttccgc cactgcggat atacgctaca aaaaagatga aaatgctcta
      541 gtgtggggta aggctaaaat gctcaacatc gcaaaaagct cttgacaata tctcgtatcg
      601 ggtgtagcat aaaagttggc gggtgtctgt gacctgtaaa ccggcgggcg acaatgagcc
      661 tgctggccct gggcggactg gcgctgatcc gaagagagag aaattcccat aactaatccg
      721 tcggtggaag gaccccatat tgcgatggga aaacagaaac aggcgatgaa taacaggttt
      781 ttcctgagcc ccaggaggca accatgtttc ttgagaaatt aaaaccatgt acgatcacgg
      841 tagttacggc aatattgatt cagacccttt tcatggcggc tccggtttcc gcggatgtct
      901 acggcatcaa gaaccggtca gccagcggcg atgggacgaa agaatacgaa cccccgtcga
      961 ttctttttca tttcgcggat agaggcggtt cgccgacgat ggtgggctat atcaccgacg
     1021 gcgacggaac ccaactgcgc gccgatggat tggcgtggtc ggtttcgttg gggatgtggt
     1081 ttttcgaacc gtccgctccg ggcgagccgg tgagttcgac gcttcgctcg ctgaatccgg
     1141 acacggcggt agccggcgcg ggcgtgacgc tgcccggccg gttcatcttc ggtgcggcgt
     1201 ttgacacgag caaccggctc tgggccacgg acgaggcgtc cgacgagttg ctccgcatcg
     1261 acccgaacac ggggagcgta ctgtattccg ttgggctgat ctatgaaggc gacacattca
     1321 atctgcataa tcaatccggc gacattgcct tcgatgcatt ggggaacgcc tggctggtct
     1381 tcgaggacgc cttttaccgc gtggacaccg ccacgggcgt gatgacgtac gatcgcacgg
     1441 tcagcggcat ttccctgtat atggtgggtg cggcgtttag ccccgtcgat gtcgatgatc
     1501 tgtacgtttt cgacgtgacg gcgggctgga acaacgacga tattcttcgc ttcgacctgg
     1561 gaaacgacta cgcccgcacg gacgtggcgt tgtatatatt caatgacatg aacgctgggc
     1621 gaggagacct ggcgtcgccg atggtccctg aaccggcaag cttaacgata ttgacgctgg
     1681 gcgggctggc gttgttccgc agacgaaagg gatgacgcac aagcggcaat tcgcagtgcg
     1741 agcaacacct tggatgcctg tgcttcgctg cgacttgcgt ggtcgcgcaa gtttcactgc
     1801 cgcacccgac tgcgtaccgt agtgcatcac gcgccgatga attgctcatg aattcgtcgt
     1861 actcccaaac gcacccgcat cgccaatctc tctaacgccc ccttcgaatc cgcctccgcc
     1921 aggcgggcca gacatgccca ggttcgcgtt cctctcgcag tcccgaaaat cgcgcgaaac
     1981 agaggcaaaa cggacttgat gaggaggccg gaacgagccg tcatgtgctc gctggatagc
     2041 cacgaacgtg gataacggga ttgcgccagg caacctgcga ggggaggcgc aaatgagttg
     2101 agttactgaa gggtgctggg gccgtggagg ggtcagtctt ccggtcggtc cccaggtggc
     2161 gacatcttta ccaagcgagg catgaacctg gccaccggac gaaccagcgt ctcctgggcg
     2221 gccatgacct tttcgatgtc cttgtagacc cagggggcct cgtccaggcc tgcggacaag
     2281 agggttacac ccttttcttt caggaactgc ctgacctggg gccacttgaa agtccggatg
     2341 gcctcgcccc gggacatcaa ccggccagcc ccatgcgagg cggactgcaa tgactcctcg
     2401 ttgccgagcc cctccaccac ataggccggg ctggccatcg agccaggaat gattcccagc
     2461 acgcccttgc ctgccggcgt ggctcccttg cggtgaacaa tcaccgtacg tccgccgtgc
     2521 tgctccttcc aggcaaagtt gtggtggttc tcgatctgca ggaggggcgg ctcaccgacc
     2581 gctttgacga tctgccggtg aatgatgtga tggttggccg aggcatagcg acccatcagc
     2641 tccatgaccg cccagtactc gtcgcccgga ccgtccagtt caagccaagc caaatgacgc
     2701 aactccttgg gcatcgattt gtgccggctc atggcccact tgctgtagaa gcctgcggcc
     2761 tcgttgcccg gaccgcgact tccgctgtgc gaaaggacgg cgaggtattt ccctggcgac
     2821 acaccacgca ccgattccaa aaacgtcacc tcgccaaact cgacgaagtg gttacccgaa
     2881 ccggaggtgc cgagttgccg gtgggctttg tctttcaggc gggcggtcac ctgcgagatg
     2941 ttccagtcct cgtccatcac cgggtgttcg cgtttcggct tccactcagc tccaataccg
     3001 aaccgggtgt tgttttcgat gatcgccttg aaacgatcat cctgatcctg cagcagatgc
     3061 gggggcaggt cgaaaacggt catcatcatc cggcaggcga tatccacgcc gacggcgtag
     3121 ggaatgaccg cgttgtcggt tgccagtacg ccaccgatgg gcaggccata gcccacatgg
     3181 gcgtcgggca tgagcgcccc ggccactgaa atgggcagct tgcacgcatt tcgcatctgc
     3241 tggtgggcct gttcgtcgat atcgtctccc caaaccttgt attccaggga ctcattgagc
     3301 ttgtagtcag cggctgtcgg cttgggctgg gcaacgagac cggccgcgat caacgctttg
     3361 gcgatcttgc cgaacaaggg gtcttccaca taagggtgag cgtcgatcaa taacatgcgt
     3421 aacgtctgaa ggacttttga cttacgttgc cccgaaacgc gcgcctcgcc tgccgccgac
     3481 agggccagat gcagaatcgg cccggccgga taacccatct tgatcagatc gcgcgacttc
     3541 atggcgtttc ctcagagaac cagcattgca tcgccatagc tatagaagcg ataccgctct
     3601 cgaatcgctt cattgtaggc attcaggatc gcctcgcgac cggcaaaggc gaacaacatc
     3661 gcgagcagcg tgctacccgg caggtgaaaa ttggtcacca tgccttctac gactttgaac
     3721 cggtacgggg gatagatgaa gagccgcgtc caaccgctgg ccatggtgac gcgaccgctc
     3781 tcgtcggcgc atgtttcgag gacgcgggcg ctggtggtgc cgatggccac aatcggccgg
     3841 tcctgcgctt gggcagcgtt gacggcctcg gctgtcgccg cagggcaatc ataccactca
     3901 gcgtgcatct cgtgctcagc cagatcatcg cagcggatgg gggcgaaagt gcccacgccc
     3961 acgtggagcg tcacgaaaga ggtctcaatg ccagcggtcc gcagctcgtt cagcacggca
     4021 ggcgtgaaat gcagcccggc cgtcggcgcg gctacggccc cgggccggcg ggcgtacacc
     4081 gtctggtatc gatcgcgatc gagtctctcc ctctcgtcct tgccggcagt actgttgtcc
     4141 tgcgtgccgc gccggatgta cggcggcagc ggtggatgac cgcactcggc caggatctcc
     4201 accgccggcg cgcagggcac cggttcggcg aaccacaccc ccgctttagc actccttgtc
     4261 aaccgcagcc ggcgagtcga gccgttgatc cgtaattcct cgcccgtctt gagccgacca
     4321 gaagcggtca acatgatctc ccacgctcca gcctcggtct cccggagaaa caagccttcc
     4381 acccgcccgc cgcttcggcg agccatgtat aaccgcgcgg gcagcacgcg cgtgtcgttc
     4441 atgaccagca gtgccttggg ggggagcagg tgtggcagcc tggcgaacac ttcgtgtcgc
     4501 agcgtaccgg tggaccgatc cagcaccagc aaacggctgg cgtcgcgctg ctccggcggt
     4561 tcctgggcaa tcagttccgt cggcagctca tattgcagtt cgcttgtacg cacggtgcag
     4621 tctcacccca gcgacaggcc aatccccttg cgggacaggc cgaatcggcg tttcggcaac
     4681 acgaagacaa taccacgacg tgttcgcggc ggaaacacgc attcgttctg gtaatacctg
     4741 ggctcaggag ccccgcgagg ccggccatcg aacccgaacc ttgctctcgg tgcagaccag
     4801 aaagacaccg ctccatcgtt cgacggcctc attcctgaag atgtcacgga ttttctcgat
     4861 caggtatcga cgcgagggct cgcggagacg ggcaaggaga atgccgtggt gcgtgccagg
     4921 cctgaaaacc ctgagatcgg agaagtcgag atcctgggtg atcaggaacc gtcccgattc
     4981 ctgggctccg ctccagacgc ggggggtcag gagacccttt tagcccctgc tcgggcacag
     5041 tatccacgtc gtgtcccaag gcggaaagct cacccacaag ccgggccggc aggttctcgt
     5101 cgagtttgag cctcatgtca gatgggggac gccaggagtg ggcagatctt cgctggcgga
     5161 agcagccgcg aaggcaatga ctgcttcgac ctgcgatctc gtaagtgtgg ggagatccgc
     5221 caggatttcg tcgatcccat ctccatccgc caggctcgcc agcacggtct ggagcgtcac
     5281 ccttgtgccc cgaatcaccg gttggccacc gcagacccgc ggatcgcgga ctatgaactg
     5341 ttggtaatcc atgacctgca tcaccttgcc ttgattgtac gacaaggtaa cggagatgcg
     5401 caatggcgga tgacccaggt accgccgacc ggtgggccaa cgcgtacagc tactcgtcaa
     5461 cggaccgctc ggcccaaagg aatgtgcgac cccagactca agacgaatct gggccacccc
     5521 gcagttcaca cgtactgcgc cggaaacctc cacggctcgc ggtagacggg atttacgtgc
     5581 ttttgggcct cgggatcgtt agggatcgtc tcggttacgg ggtcaaactg caccgaacgg
     5641 ccaagtttca gcgaaagcat ccccaggttg atggccatat ccactttgta gtgatagccg
     5701 acgtggcaac tgggcggcgt gcgcgtgcgg atgccgtcga gccattcgcg atgatgaccc
     5761 ggcgagtcgg gcgtgacctt ctcaaccttc tcgacgtcct cctcctccat gcggtcgcct
     5821 tcggggacaa tcttcaggta accgtaatcg gcgatcaacg tgccattggt cccgtggaag
     5881 tagatgccgc gccggcggtg cacgccgggc tgtccctgcg tgtcaaaccc gaagctgttg
     5941 accagcgagg tccaccagca cacggtcagc ttcgggaact gccactgaat ctgctgaaaa
     6001 tcgtaggcgt cgccatcgtc gtcgatgatg tagcggccgc ctgtgctgct ggtgcacgtg
     6061 ggatggccaa ggtccaaggc ccagtacggc aggtcaagca ggtgcggggc catgccgggt
     6121 gtccaacccc cgctgtaagc catgaacgat gggtgaaact ccgaattctt gaacagcagt
     6181 ggatgaaacg ggcgtttggg tcccggtccc agccacatct cccagtcaag gccttggggt
     6241 accggggtcg gcggtgtcct gccgatcccc tcaggcccct ggttgaggac gtggaacaca
     6301 cgaaccatgc tgatcggccc gagaatgccc gagcggacga cgtcgacaat tcgccggtaa
     6361 ttggccgtgg catggacctg tgtgcccacc tgcgtgatgc gcttgtactt ctggaccgcg
     6421 tggtgcactg ctaggcactc gcctacggtg agggtcattg gcttttcgag gtagaagtcc
     6481 ttgcccgcct cggccgcttc gatggccatc agcgcgtgcc agtgcggggg cgtggcgatc
     6541 accacggcat cgatgtcctt gttttcgaga accttgcggt agtcgccgta cggcttggcc
     6601 gtcccgttgc acttggcgag agcctcgtcg cgacgtttct cgaagacctc gcataccgcg
     6661 gtgatgaccg cgtccggctg ctgcatcaac tggtcgaggc tgaacttgcc ctgcccgttg
     6721 gccccgatca gcccgatccg gatcttgtcg ttggggctca ctcgggcagc cgccggtctt
     6781 gtctcggccg ccccggcccg agacccgatg acgtacggtg cggccagtcc tgccgccgct
     6841 cccattgtcc cgctcaagaa aacccgtcgc gataccgcgt cagtggcgtt gccggacttg
     6901 ctaccgcttt gcatgattga cccttcgaat aagaaaccag tgtttgtccc aggcgaccgg
     6961 acgctcctct gctgccctaa ggcaggtcgc cttcctgcgg gccgcaaccc taccgcactg
     7021 cgatccagaa acctgcgtgc aagccgcaca gatgtccggc tacaaggttc caataccaga
     7081 tgagactaac caactgaccg ggccggttca atgccggcgt gctatcagtc gttctgttgg
     7141 gtgcgtgacg acttcgatcc ggccggtcag catgttgccg gggccttcag gatggatttg
     7201 accgttccct gtcacggcag ccaattcttc agcccgcaat gactttgtct atcagttctg
     7261 ccagttgagc cagacgagct ttcttgccgc ctttgacctc ggcggtgatg tagctgctgt
     7321 agcccacctc acgcagggcc ttggtgaccg acggccagtc gcagtcgccc tccatgagtt
     7381 cgacgtcgaa gcccgcccaa acgccctctt cgtttgcctt ctgccggctg tactccttga
     7441 tgtggatctt gcagatacgc ttgcccagaa tgcggaccca ctgttccggc cagccgtagc
     7501 ggaccacgtt gccgcagtcg aagtacgctt ggaccatggg tgaattgatc tcgtcgatgt
     7561 aacgggcgaa ctccagcggg ctgagcagga agttgttcca gacgaactcg atgccaataa
     7621 cgacgccgag cttctcggca atgggggcca gcttgcggat ctcggcctga gaccgggtat
     7681 aggcctcgtc gtaggcgatc tccttgctga ccacggccgg gacgagcagg acggagccgg
     7741 cgccccagta cttggcatcc tcaagcgagc cctgcatgct cttgcgacat tcctcgcgga
     7801 ccttgggatc gggatgcgac agcggcttct tccagtgatc ggggttcatc acgctgtgga
     7861 tctcaagacc ggtcgcgtcg cgggccttga ggaactcctc gcggtccggt cgtccgctgc
     7921 tctgaacttc gacaccgtca aagcccagtt ctttaagcag cttgaacttg tcgagcaccg
     7981 agaggtcctc gccgaccatc tcgaaaccga tggccttctt gaacttcgcc gccggcttgg
     8041 ccgcgggtac cgccgtgttt tcggctccgg ccgccaggtg gccccctcct acggccatca
     8101 gcccggcacc caccagcttc acgaaatccc gccgatcgat actctgctcg atgctcatta
     8161 tgatccctca ggtcttgcgc tgcctcaatc cgatcgttaa ccgacttggg tacggattct
     8221 agacggccat cgcctatgcc gcaaccgaac aggctcggac atgctgcccc gggtttgtga
     8281 cgcattcggc gggcgtgtgc tccccaggta ccaccgacag tctgctctcc cggtgtagcc
     8341 ttgccgaagc cgggcccgcc gatgcagacc ggcatcagtc tcgtcgcctg tggccgcaaa
     8401 tcgataacag agcgaaaccg gccagcattg cgattcccga aacaggcccg attgtgccgc
     8461 acccgcccgg tgttcgatcg attcccactc cgtaagcctt gagcgagacg accacgtcac
     8521 cccagtttga acgcacgatc agctctccca ttctggttct ggcgatggta tccggggagg
     8581 cgatgactcc aaaggtccgg tgctccccgg gcggaatcgt cgccggcagg tcacccatcg
     8641 gcacttgcga ttcggtcag
//
LOCUS       DAOTPW010000247         8560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7461_length_8560_cov_5.632922, whole genome shotgun sequence.
ACCESSION   DAOTPW010000247 DAOTPW010000000
VERSION     DAOTPW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7461_length_8560_cov_5.632922"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="P5316_18025"
     CDS             complement(<1..1650)
                     /locus_tag="P5316_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86911.1"
                     /translation="MKRTIWILTICVAATAASTALAQDCVWRPWAAGWSVINKGPDLY
                     PNNKIYDPTNWDYSFSQLTLLNTPKTTPDGSTMALHWFLDAQPPATKSNGSGSGGVYQ
                     EITVTPGVPLNYSFWWRGSSGSNTAWFEFMLIDGPFTIYDADQRSGSTNPYIMRKREN
                     VTFGWEQLTHASTSSPGPVQSTLTPTGTTVTVVLKAGRSPEGAMEVFFDDVRVWQGAG
                     SNLVVNGDFEDTAGQSVCDDQLMLQDASQDNYWFGLSQCPGAQHTSTAPVSPTSHPND
                     SDATITITGTDLHLVTQVRLVPVGGGNDLVGTNLVVGPGNTTLTASFPTNGATDGLYN
                     VVTVQSPAPPCLVQTLPAAFEVTCPTAIVVTGVSPDTVVDPGSGVTLTITGTNLDRVT
                     GASLVYRVTSSPVRNTSAIITQGDDLLASFDLICAPAGSYDLVLHRTDACREATVSQA
                     LRISKSLPASGCLWRPWAASWSRLNLGPDLDPGDAQAFDATNWDYTFGQHPNLGTALD
                     TPDESGLALHFFLDGQTGYGESGHGSGGVFQQITVQPGVPLE"
     gene            2007..3707
                     /locus_tag="P5316_18030"
     CDS             2007..3707
                     /locus_tag="P5316_18030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRR86912.1"
                     /translation="MPVNNPVQVSNPLRRDLPPALFPAACTLVLFGATGDLVRRKLLP
                     ALYNLACDGFLPPRLLTIGAARKPFSDEQFRQEMLKAVNEHSRRKPVLPGVWQDFAEG
                     LFYQQVRFDESSDYGALGRRLAELEGQGRGAKGGRLFYLATDPAFFAPVVQHLAQVGL
                     IRRAKGPMPSLGEGSAQGRVAADRVIVEKPFGRDLDSARDLNRRLAEVLDESQIFRID
                     HYLGKETVQNILALRFANAIFEPLWNRRYVDHVQITVAERDGMEGRRGQYYDSAGAAR
                     DMLQNHMMQLLALTAMEPPAGMNAQAIRDEKVKVLRAIAPLGQGACAHCAELLQRSAK
                     AFPDPGSHVDDVSDFGFCVNKQCNACVRGQYAGGMLGDVRIKAYREEECVNPESNTET
                     FVGVRLFVETWRWSGVPFYLRTGKRLAKRLTEIAIQFRQPPMAMFDAAEAIPHQRNQL
                     VLRVQPDEGVSLSLAAKMPGMRMRLQPVKMDFRYGSVFGADPPEAYERLLLDAMLGDA
                     TLFIRADEVDYAWQLLTPMLQAWEHGPAPRFPNYEPGSWGPPQADKLFISPGSGWRRL
                     "
     gene            3756..4856
                     /locus_tag="P5316_18035"
     CDS             3756..4856
                     /locus_tag="P5316_18035"
                     /inference="COORDINATES: protein motif:HMM:NF021632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HRR86913.1"
                     /translation="MSRLLSPDGAVLGDPAEVEEALAVLWDTAAGQPETEAGGPAVTR
                     VCMGNLVVVGEAKQWEQLSIVLAQIAREYPSRTVALLVGDEAMAGRPPGQVQAVVSSV
                     CHLPQPGRPQVCCERIVLRIGSQDGAGLDRLLLPLLASDVPLLGWWTVDPASCPRLLA
                     AIRQLIDRLIFDAGLPGFAGLLEPGRCVTRELGWFRSYRWRELAAGLFDETDPAVLSA
                     IDRVAVAVRGKDICDWLDALWIVAFLGGQLAWRAERVLGRGRLEFQSRGRAIEVVIDG
                     SAAAGQGLDSFEVVANGSRFRFARCHEASDEFRITLSDEYACQMPRSIQIRSRGRADA
                     LAVALTGRVVDRSYDRAVPLAVWMAEAMRAWW"
     gene            complement(4958..5572)
                     /locus_tag="P5316_18040"
     CDS             complement(4958..5572)
                     /locus_tag="P5316_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86914.1"
                     /translation="MALLLSTAACNTVPGEKFQAVQRDLLTSQERIRQLESELAAQQQ
                     TNRDLSRQIANLSRVGDISDNVLIIPERIRLASMSGGYDDDGKAGDDGIVVYIQPIDR
                     DQHVVKAAGTIKVRLLDPQNPPDRTVFAEYYFDVEHTRALWYGRLMTNHFSVKCPWPS
                     GYLPAHNEIVAHVIFTDLLSGRSLTATGTFPIQFPPKETRPAGE"
     gene            complement(5702..6328)
                     /locus_tag="P5316_18045"
     CDS             complement(5702..6328)
                     /locus_tag="P5316_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRR86915.1"
                     /translation="MSNLVPIVIEKTGRGERAYDIYSRLLRDRIVFLGGGIDDDTANV
                     IIAQMLFLSNEDSSLPISFYINSPGGSVSAGLAIYDTMQFLRCEVHTYCIGMAASMGA
                     VLMCGGAKGKRYVLPNSRLLLHQPLIGGVLEGPATDLSIEAQEIIRLRERLYEIIAKH
                     TNKPLETIAQDCDRNKWLDSKEAVEYGLVDMILDRAPEVKPSVPPSDE"
     gene            complement(6406..7029)
                     /locus_tag="P5316_18050"
     CDS             complement(6406..7029)
                     /locus_tag="P5316_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRR86916.1"
                     /translation="MNHPEDILRIIDQQMPPYQRYREMSIEDMLLENRIIFLAGPIHD
                     RTASVVIMRLLYLQSIKPDQPINLYINSPGGLVDQTLAIYDTMQFLSCDIGTYCIGQA
                     ASGAAIILAAGTKGQRYALPNAKIMLHQPYSGITGQAEDIRIQAEEILKDKKLLNSIL
                     AKHTGQDPEKIAKEIERDRFMDAKEALAYGLVDEILEHEKDKKKKKS"
     gene            complement(7193..>8560)
                     /gene="tig"
                     /locus_tag="P5316_18055"
     CDS             complement(7193..>8560)
                     /gene="tig"
                     /locus_tag="P5316_18055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRR86917.1"
                     /translation="EVVDVQVEDLGGLRKKLTVTVPHDTIGEQLDEQYGQMRNEAVVP
                     GFRKGRAPRRLLEKRFGRDVNATLIQQMLGTGYMAAVDKADLKVIGDPLIWVKRKEGE
                     GEELVDVQTAMERVKLPDDGPLTFSCEVEVRPEFDLPEIEGIPLEKPVVTVTDEDVQN
                     QLERLRTTRGTYEVVPEDGAVEANDVLYVDFRMTCEGTVLKEERMVRVAARGQVIDGV
                     TLEKLGEALAGAKTGETRTASGQVPDDHVKAEFRGKQVDFEFKIREIQRLRLPELNEQ
                     FFKSLGFEGEQDLRDYIRKDLESRIEEQVRRVMAGQVYKYLEDKTSFELPPRLSERQA
                     DRVLIRHMVELYRQGVPQAEVEKRIDEIKTSSQELAAREMRRFFIMEKVAEQLEDIEV
                     SEGEINNLIATIAYRQGRRFDRVRDELAKGGGLTNLYLQIRDEKLIERLIEKAQITEK
                     TREAN"
BASE COUNT         1538 a         2560 c         2631 g         1831 t
ORIGIN      
        1 ctccaacggc actcccggct gcaccgtgat ctgctggaac acgcccccgc taccgtgccc
       61 gctttcgccg taaccggttt gtccgtccag gaaaaagtgc agcgccaggc cgctctcatc
      121 cggtgtatcc agcgcagtgc cgagattggg atgttggccg aaggtgtagt cccagttggt
      181 ggcgtcgaac gcctgcgcat ctccgggatc gaggtcgggg ccgagattga gccgtgacca
      241 acttgcagcc caaggcctcc acaggcagcc actcgccggc aggctcttgc tgatccgcag
      301 ggcctgactc accgttgcct ctcgacacgc gtcggtgcgg tgcaagacga ggtcataaga
      361 cccagccggc gcacagatca gatcgaagct ggcgagcaag tcgtcgccct gcgtgattat
      421 cgcgcttgta tttcggacag gggaggaggt cactcgatag accagtgaag cacccgtcac
      481 ccggtcgaga ttcgtaccag tgattgtgag tgtcacaccg gagccgggat cgacaaccgt
      541 atccggcgaa acgccggtca cgacgatggc tgtgggacaa gtgacctcga aagcagccgg
      601 cagagtctgg accaggcacg gaggtgccgg cgactgcacg gtgaccacgt tgtagaggcc
      661 gtcagtggcc ccgtttgtgg ggaagcttgc ggtcaaagtg gtgtttcccg gaccgacgac
      721 caagttggtg ccgacgaggt cattgccgcc gccgacaggt accagccgta cctgcgtgac
      781 gaggtgcaaa tcggtgcccg tgatggttat tgttgcgtcg ctgtcgttgg gatgacttgt
      841 cgggcttacc ggggcggtgc tcgtgtgttg cgcgcccggg cactggctca ggccgaacca
      901 gtagttgtct tggctcgcgt cctggagcat caactggtca tcgcagacgg actggccggc
      961 ggtgtcctca aagtcgccgt ttacgacgag gttggaaccc gcgccctgcc agacccgcac
     1021 atcatcgaag aagacctcca tcgcgccttc cggggatctg cccgccttca ggaccacggt
     1081 gaccgtagtc ccggtgggcg tcagcgtcga ctgcaccggg ccgggagacg aggtgctggc
     1141 atgggtcaat tgttcccagc cgaaggtcac gttctcgcgt ttgcgcatga tgtaggggtt
     1201 cgtggagccc gaccgctgat cggcgtcata gatagtaaag gggccgtcga tcagcatgaa
     1261 ctcaaaccat gcggtgttcg acccactgga ccccctccac cagaacgagt agttcaaagg
     1321 aacacccggc gtgacggtga tttcctgata gacaccgcca ctacccgacc cgttgctctt
     1381 tgtagccggc ggctgggcgt ccaggaacca atggagagcc atggtgcttc cgtcgggcgt
     1441 cgtcttcggg gtgttcagaa gggtcagttg gctgaacgaa taatcccagt tggtcgggtc
     1501 gtatatcttg ttgttcggat agagatcggg gcccttatta atgacgctcc aaccggctgc
     1561 ccagggtctc cagacacaat cctgagccaa ggccgtcgac gcggccgttg ccgcaacaca
     1621 gatcgtcagt atccatatag ttcgcttcat tgcctggtac tccggtcact gaggttttgt
     1681 agctcctgcg gggcgcaaga cgggatggtc tcgggcttcc ctccgaaggc ctctttccca
     1741 agaccgctcc ggatcgatcg agagttacat tgagacccca tccgtttcct tcctctccgc
     1801 agctctccgc gacttacctg aattgtagca caatgacatc actgatgcca actgcttttc
     1861 ggacgttcta tgggcttttt cggcttatcg tggttgtgcc gtgagcatca gttgcgtacc
     1921 ctcaatgcag tatgagtccg cgcgatgagt tccacgcgtc ggtcgtcatg agcctttttc
     1981 gcagtccttc ttcaagggtt gtgcgcgtgc cagtgaacaa tccggtccag gtttccaacc
     2041 cgttgcgccg cgatcttccc cccgcgttgt ttcctgcggc ttgcacactc gttctattcg
     2101 gggctactgg cgatctggta aggcgcaagc ttctgcctgc cctatacaac ctagcctgcg
     2161 acggattctt gccgccgcgg ctcttgacca tcggggcggc tcgcaaaccg ttttcggacg
     2221 agcaatttcg ccaagaaatg ctcaaggcgg tcaatgagca ttcccgacgc aaaccagtgc
     2281 tgccgggcgt gtggcaagac tttgccgagg ggttgttcta ccagcaagtg cggttcgacg
     2341 aatcaagtga ctacggggcc ctgggccggc gactggctga gctggaaggc caagggcggg
     2401 gggccaaagg ggggcggctg ttctacttgg caaccgatcc ggccttcttt gcgccggtcg
     2461 tacagcattt ggcgcaagtg ggcctgatcc gccgagcgaa gggacccatg ccgagcttgg
     2521 gtgaggggtc cgcccagggg cgagtagccg ctgaccgcgt gatcgtcgag aaacccttcg
     2581 gacgggactt ggacagcgcc cgggatctca accgccggct cgccgaggtg ctggacgagt
     2641 cccagatctt tcgcattgac cactacctcg gtaaggagac tgtccagaac atcctcgcgc
     2701 tgcggtttgc caatgccatt ttcgagccgc tgtggaaccg ccggtacgtg gatcacgtcc
     2761 agatcaccgt tgccgaacgt gacggcatgg aaggacgacg cgggcagtac tatgactctg
     2821 cgggtgcggc gcgggatatg ctgcaaaacc acatgatgca actacttgcc cttaccgcaa
     2881 tggagccgcc ggcgggcatg aacgcccagg ccattcgcga cgagaaggtc aaggtgctcc
     2941 gggccatcgc tccgctcgga cagggagcct gtgcccattg cgccgaattg ctgcaacgat
     3001 cggcgaaagc ttttccggat ccggggtccc atgtcgacga cgtctcggat ttcggcttct
     3061 gtgtcaacaa gcaatgcaat gcctgcgttc gtggccagta cgccggcggg atgttgggcg
     3121 acgtcagaat caaggcctat cgcgaggagg aatgcgtcaa tccggagtcc aacactgaaa
     3181 cgttcgtcgg cgtccgcctg ttcgtggaaa cgtggcggtg gtccggagtg cccttctact
     3241 tgcgaaccgg aaaacgtctg gccaagcgac tgaccgagat tgccatccag ttccgacagc
     3301 cgccgatggc catgttcgac gccgccgagg ccatccccca tcagcgaaac caactcgttc
     3361 tcagagtgca gcccgacgag ggcgtgtccc tgtcgctggc tgccaaaatg cccggcatga
     3421 gaatgaggct ccagccggtc aaaatggact tccgatacgg cagcgttttc ggagccgacc
     3481 cgcccgaggc gtacgagcgg ctgctgctgg acgccatgct tggcgacgcc actctgttca
     3541 tccgagccga cgaggtggac tacgcgtggc aactgctgac acccatgttg caggcgtggg
     3601 aacacggacc ggcgccgcga tttcccaact atgagccggg aagttggggg ccgccacagg
     3661 ccgacaagct gttcatcagc ccgggcagcg gctggagacg attgtgaaag accggattac
     3721 cacggagctg cgaacacatg acgccggata ccaggatgag ccgactgcta tcgcccgacg
     3781 gagcggttct tggagatccc gctgaggttg aagaggcact cgcggtcctt tgggacaccg
     3841 ccgccggtca gccggaaacg gaagcgggtg gtccggctgt gacccgcgtt tgcatgggta
     3901 accttgtcgt cgtgggagag gccaaacagt gggaacaact gagcatcgtg ttggcccaga
     3961 ttgcccgaga gtatccctcg cggacggtgg cgttgctggt gggtgatgag gcgatggcgg
     4021 gccgcccgcc cggacaggtg caggcggtcg tttcgtcggt ttgccatctg ccccagccgg
     4081 gtcggcctca ggtatgctgc gagcggattg tccttcgaat tggctcacag gatggggcag
     4141 gcctcgatcg actcttgctg cctttgctgg cgtcggacgt cccgctgctg gggtggtgga
     4201 cggtcgaccc cgcttcgtgt cctcgcctcc tcgcagcgat ccggcaattg atcgaccggc
     4261 tcatcttcga cgcgggcttg ccaggtttcg caggcctgct tgaacccggc cgctgcgtga
     4321 cgcgggaatt ggggtggttc cgctcgtatc gctggagaga gctcgctgct gggctcttcg
     4381 atgaaacgga tccggcggtc ctgagtgcga ttgatcgtgt cgcggtggcg gtccgtggta
     4441 aagatatctg cgattggttg gatgctcttt ggattgtggc gttccttggc ggacaactgg
     4501 cttggcgagc agagcgggtg ttggggcggg gacgcttgga attccagtcg cgaggccggg
     4561 ctatcgaggt cgtcattgac ggtagcgccg cagccggaca aggtcttgac tcgtttgagg
     4621 tcgtggccaa cggtagcagg ttcagattcg cccgttgcca cgaggcgagc gatgaattcc
     4681 gcattacgct cagtgatgaa tatgcctgtc agatgccccg atcgattcag atccgcagcc
     4741 gaggccgggc cgacgccttg gccgtcgcac ttaccggccg ggtcgtcgac cgctcctacg
     4801 accgcgctgt tcccctagct gtttggatgg ccgaggcaat gagagcgtgg tggtgaccgc
     4861 ttccttacgg cggaccgctt ttctgcgggc cgcaaccaaa gtgcgcggtg acccgaaaac
     4921 aagcgtgcaa gccgcacggc tttctggccg caagatgcta ttccccggcc gggcgtgttt
     4981 ccttgggggg aaactggatg gggaaagtgc ccgttgccgt cagacttctt ccgctgagca
     5041 agtcggtgaa gatcacgtgg gcgacaatct cgttgtgggc gggcaagtag ccggagggcc
     5101 atgggcactt gacggaaaaa tggttggtca tcagtcggcc gtaccatagc gcgcgggtgt
     5161 gctcgacatc gaagtagtat tcggcaaaca ccgtacggtc gggcggattc tgagggtcaa
     5221 gcagtctgac cttgatggtg ccggccgctt tcaccacgtg ctgatcgcga tcgatcggct
     5281 ggatgtaaac cacgattccg tcgtcgccgg ctttgccgtc gtcatcgtag ccgccgctca
     5341 tgctcgccag cctgattcgc tcgggaatga tcagcacgtt atccgagata tccccgacgc
     5401 ggctgagatt ggcgatttgt ctgctcagat cgcgattggt ctgctgctga gcggctaatt
     5461 cgctttcaag ctgccggatt cgctcctgcg aggtcaagag gtcccgctgg acggcctgga
     5521 acttctcgcc tggcacggta ttgcacgcgg cggtcgagag cagcagagcc atggcgcccc
     5581 agaggccgat gcggcctgta gctgggctga cgaagccagt gcagggggaa caagcggcga
     5641 tctgacggca gatggatacc actgtccgcg tccttcacac ctcgatcggc gggcggatca
     5701 actattcgtc cgaaggcggc accgagggtt tcacctcggg tgcgcgatcg aggatcatgt
     5761 ctaccagacc gtactcgacc gcctccttgc tgtccagcca cttgtttcgg tcgcagtctt
     5821 gggcgatcgt ctcgaggggc ttgttggtat gcttggcgat aatctcatag agacgttcgc
     5881 gaagccggat gatctcctgg gcctcgatcg acaagtccgt cgcaggaccc tcgagaacac
     5941 cgccgatcag cggctgatgc agcagaaggc gggaattcgg cagcacgtac cgcttgcctt
     6001 tggctccgcc gcacatcagc actgctccca tgctggcggc cattccgatg cagtatgtgt
     6061 gcacctcaca acggaggaac tgcatggtgt cataaatcgc caagccggcc gagaccgatc
     6121 cacccggcga gttaatatag aagctgatcg gaaggctgct atcctcgttc gatagaaaga
     6181 gcatctgcgc aatgatcacg ttggccgtgt cgtcgtcgat gcctccgccg aggaagacga
     6241 tgcgatctcg cagaagacgc gaatagatgt cataggcccg ttctccgcgg cctgtcttct
     6301 cgatcacgat gggaactaga ttgctcatgg gggctttcca tatcagacgt tcgattcgat
     6361 catcacgcca acgggctgag ccaatgctcg cccgcggtca cggcctcacg acttcttttt
     6421 tttcttgtct ttctcgtgtt cgaggatctc gtcgaccaat ccgtacgcca aggcctcctt
     6481 ggcgtccatg aaccggtctc gctcgatttc cttggcgatc ttctcgggat cctggccggt
     6541 atgtttggcc aggatcgagt tcagcagttt cttgtccttc agaatctcct cagcctggat
     6601 gcggatgtcc tcggcctggc cggtgatacc actgtaaggc tggtgcagca tgatcttggc
     6661 gttcggcagg gcgtaccgct ggcccttggt gcccgccgcg aggataatgg ccgcaccgct
     6721 ggccgcctgt ccgatgcagt aggtgccgat gtcgcagctc aagaattgca tggtgtcgta
     6781 gattgccaag gtctggtcca ccaggccccc gggcgagttg atatatagat tgatcggctg
     6841 gtccggcttg atcgactgca aatagagcaa acgcatgatc acaacactcg ccgtccgatc
     6901 gtggatcgga ccggcgagaa agatgatgcg gttttccaac agcatgtcct cgatggacat
     6961 ctcgcgatac cgctggtagg gcggcatctg ttggtcgatg atccggagaa tgtcctcagg
     7021 atgattcata agattccttc caaattacgg agccgacgtc ccggcgtcat gcggtttcca
     7081 gccgtattat tccatggcgg cgagccgttg taacctactg acccacacca tcatcggcca
     7141 actcgatcat catcttgctc ttggggtcta ccgatgatac cgcgggtcgc cgtcagttgg
     7201 cctcgcgggt tttctccgtt atttgagcct tctcaatcaa ccgttcgatg agcttctcat
     7261 cgcgtatttg caggtacaga ttggtcaggc cgcctccttt cgccaactca tctcgaacac
     7321 ggtcgaaccg ccgtccttgc cggtaggcga tggtggcgat cagattgttg atctcgcctt
     7381 cgcttacttc gatatcctca agctgttcgg caaccttctc catgatgaag aaccgcctca
     7441 tctcgcgagc ggccaactct tgggaactgg tcttgatctc gtctattcgt ttctcgacct
     7501 ccgcctgcgg aacaccctga cggtagagct cgaccatgtg ccggatgagc acgcggtcgg
     7561 cttggcgctc cgacaaacga ggggggagct caaacgatgt cttgtcttcg aggtacttgt
     7621 aaacctggcc cgccatcacc cggcgaacct gttcttcgat tcggctttcg aggtccttgc
     7681 ggatgtaatc gcggagatcc tgctcgcctt cgaagccgag gctcttgaag aattgctcgt
     7741 tgagttcggg taacctgagc cgctggatct cgcgaatctt gaactcgaag tcgacctgtt
     7801 ttccacggaa ctcggccttc acatggtcgt cggggacctg cccggaagcc gtgcgcgttt
     7861 cccctgtctt ggcccccgcc aaggcttccc ccagtttttc cagcgtcacg ccgtcgatga
     7921 cctgtccacg ggcggcgacc cggaccatcc gctcttcctt aagcacggtt ccctcgcagg
     7981 tcattctaaa atcgacgtag agcacgtcgt tggcctcgac cgctccgtcc tcaggaacaa
     8041 cctcgtaggt gccgcgggtc gtgcggagcc gctccagttg gttctgaaca tcctcgtcgg
     8101 tgacggtgac caccggcttc tccagcggaa tgccctcgat ctccgggaga tcgaactcgg
     8161 gtcgaacctc gacctcacag gaaaaggtca gcggcccgtc atcgggaagc ttgactctct
     8221 ccatggccgt ctggacatca accagctctt ccccctcgcc ctcctttcgc ttgacccaga
     8281 tcagcgggtc gccgatgacc ttgaggtcgg ccttgtccac ggcggccatg tagcctgtgc
     8341 cgagcatctg ttgaatgagc gtggcattca cgtccctgcc gaaacgcttt tccagaagcc
     8401 gtcgcggggc tcgtcccttg cggaagcccg gcacgacggc ctcgttgcgc atctggccgt
     8461 attgctcgtc gagctgctcg ccgattgtgt cgtgcggcac ggtgacggtc agcttcttgc
     8521 gaagcccacc gagatcctca acctgaacgt ccacgacctc
//
LOCUS       DAOTPW010000248         8533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7484_length_8533_cov_6.509200, whole genome shotgun sequence.
ACCESSION   DAOTPW010000248 DAOTPW010000000
VERSION     DAOTPW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7484_length_8533_cov_6.509200"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1315..2463)
                     /locus_tag="P5316_18060"
     CDS             complement(1315..2463)
                     /locus_tag="P5316_18060"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRR86918.1"
                     /translation="MSTLSGVRPAGDMLSIPGVMLEFEKPLLRIEREIAELEAVQNTT
                     QRDLSAELKELRTTLESMMRRTYARLTPWETVLVARHPRRPGLRDYIEGCVKDFCELS
                     GDRCFGNDKAIVTGFGRIGGQKVMIVGHDKGRETKEKIERNFGCPHPEGYRKALRKMK
                     LAEKFRLPVVTVIDTQGAYPGDKAEERGIAQAIAVNLMEMSRLRVPIVGVVIGEGGSG
                     GALGIGVTDRLAMFEHAYYSVITPEGCAAILWKSTEQVAEAARHLKLTAQDLKKLDLI
                     DKILPEPLGGGHRNPSAMIATFEQYVSETLRELKRVRLDTLLKRRYERWRSMGNFFEN
                     LNAPKTSPRSRTRAPSGNGNGAAGNGQNGNGRARIVERRKSEAADAVD"
     gene            2639..3535
                     /locus_tag="P5316_18065"
     CDS             2639..3535
                     /locus_tag="P5316_18065"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRR86919.1"
                     /translation="MHKIIDIKNLDQIAIIGVGLLGGSIGLALRAAGFGGCRIGAGRR
                     ASSLRKALNCDAVDVVTRDVAKAVSGSQLVILCTPIRQFDGLITRMAPALSPGTYVTD
                     VASTKAQVVRMAERLLPDPVRFVGSHPMAGSEKTGVEFARADLFQLSTCILTPTSRTK
                     PATVSWMRRFWEALGCRTVVTSPERHDRLLARVSHLPHAVAAALVNLAVCESAIDLAG
                     PGFADATRIAGGDATMWTDIFRTNRRAVLQAIDRLVAELRWFRQKLDDDDAQAILKWL
                     AAGQEARNKWISRRYRKKVLPP"
     gene            3532..6558
                     /locus_tag="P5316_18070"
     CDS             3532..6558
                     /locus_tag="P5316_18070"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRR86920.1"
                     /translation="MSQHTASPLWRITVSPKRAELDSHGAAILRDIHELGLPHVRVVA
                     SSRLFLMETDLPETVLERIASLLLADPITESYRLHRGGDEREPAHDDSDCEIEVHLKG
                     GVMDPVAASTLAAVADMGIDTRALAISTARLYRLQGLANLDELEFVARRLFYNDCIEE
                     AYLSGFGRHDVRPQHLPVAPKRDFELRYVPLRRLDDVALRKLSREAHLFLSLEEMKTI
                     QDHYRTLGRDPTDLELEMFAQTWSEHCVHKTLKSAVRYRGAAFPKTSSASQKMLPDGT
                     VEVCYENLLRDTVARATNDLTGSGRVNWCLSVFKDNAGVIEFDKDYAVAFKVETHNHP
                     SAIEPYGGAATGIGGCIRDVMGVGLGAKPIANTDVFCVGRPDFPMNLLPKGVLHPRRI
                     LKGVVAGVRDYGNRMGIPTVNGAVVFDNRYLGNPLVFCGCVGLMPNRCIEKKPNPGDL
                     IVLIGGRTGRDGIHGATFSSSELTDTHADEFSHAVQIGDAITEKKVLDAMMRARDHAD
                     GCLYTATTDCGAGGLSSAVGEMGEKLGADVELSKVPLKYAGLRYDEIWISEAQERMVL
                     AVPPGNWEKVRAICEAENVEATVIGTFRNDGRLIIKYDGKQVGDIDARFLHGGVPKTR
                     REATWKPAVSGATPLDPPLARGEGVLGAMPTAPRGHANMPGGRDLLLSRLSDLNTASK
                     EWIIRQYDHEVQGASVVKSLVGPGLGPSDAAVLRPRLSSDKGIVLANGICPSLSDVDP
                     YWMAVAAIDESLRNVICVGGDPNRTAILDNFCWGNCEDPQTMGALVRACQGCYDAAVA
                     FGTPFISGKDSLNNEFALDANDARQLGEMMPLFNNRIRIPETLLISAMSVIDDVKRCV
                     TMDAKPSDQPTLFFYVGLKARQWSEALIKDAAGLHAAVSELIRSGKVLAAHDCSDGGI
                     LQALAEMAFAGQCGIEATFTDLCPFSPGTSGYILQVPESAANDVARIAEQRAGVFARR
                     IATTARNEKLIWRAGDRRSEVPLADLAKAWRASLNW"
     gene            6803..7921
                     /locus_tag="P5316_18075"
     CDS             6803..7921
                     /locus_tag="P5316_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR86921.1"
                     /translation="MSWMVILMASLLQAAAGQSQEATFVEINGEKYGAQADDFGPIGG
                     GRGYKQIIGKGDLTVKNLDQLIDALGKAKAGQVVFIPGETEIDCTARVYIEELVLQVP
                     AGVTLAGNRGENGSVGALLCSDALKTPLLIRAAGPDVRVTGLRIRGPNPKRYLDHHHR
                     SFAEGRGHEYYYKFPTSAGIGTEHPGLEVDNCEISGFSHCGVKLSRGDRHHIHHCFIH
                     HCQYNGLGYGVSHDTASSLIERNLFDFNRHSIAGTGRPGCSYEARHNVELGTSLSHCF
                     DMHGGRDRKDGTDIAGTRIAIHHNTFRAPEHAVAIRGVPQETCEVFGNWFPRHDSPQA
                     AVRASAGTHVRNNAYGAAPAVMDPQPQAREKAAATQPG"
     gene            complement(7996..>8533)
                     /locus_tag="P5316_18080"
     CDS             complement(7996..>8533)
                     /locus_tag="P5316_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86922.1"
                     /translation="ANVVSIDSADWTELDNVLMSYGQTVTSAEEAASQPPTTLASYYD
                     LTVMDWLWVRLTPDGSDVTAWGMDVDSLLGVDPPTVWALTQDLTAVNGPIAAPTAEPP
                     IESQESAQWFTTTAGIGGVTREPLPAGQTVLATLSLTISPEPGTYHLTLTDAQFVDTI
                     HVTHPATTGQPLVIVVGQ"
BASE COUNT         1556 a         2640 c         2735 g         1602 t
ORIGIN      
        1 cctggccgac gccccttgcg gggcgcgggc acaaggcact gcctctccat aagtaacttg
       61 ccggttttct gtgaagcagc gtcttgacgc acgcgcgtca atcaggttgt cacggactcg
      121 ccatcgagcc cgtattcaaa ctcatccgag gccacacacc tccctatcca agaccacgct
      181 tcctgtctaa ctcctgcttt attatatgtt gccgggccga acacatcaag gccattctcg
      241 gccgttggtg aagggcgcgg cactccgaac agctttgcgt ccggcgctcg cccagagcga
      301 ggccagctca tcagggcaag ccaagcaggc aaacaaccag accgcagagc gacccgaccg
      361 caaagggccg ccaagaaaaa tcctgtggtc tcggccagcc cagactcaca tggtttccct
      421 cccaacagct tgccaatctt cactggctta cccaagtttg gcttcctttg gcgcgagagg
      481 ttgtcggcat tcggcactca gcggtcagcc agacacgcct ggtgaacttc tcatcggccg
      541 aacgctgatg gctgatcgct tgcatccttg ggttcgtttc gcattttgcc gttgttcgct
      601 gcggtgtcat cgcgcccaca cgccctttgc gaaccgatcg cagagtcatg gagcctccaa
      661 ctccagggat aacactccgg gtgttccgtt ccgcacacgg attccggagc aggggcaact
      721 agaaggcccc agaaaggatt ctggggccag cgatgtgggc accagaaggg attctggggc
      781 cagcggagca ggggcaacta gaaggcccca gaagggattc tggggccagc gatgtgtcag
      841 acgtgccgac gggggcgtcg gcaccacaat gaatgcccaa cgaggaattc ggtgcgaagc
      901 gtccgatccg gacatgcttc tgtgcgcgcc tgcggggatt gtcatgcaac cgggggatcc
      961 aggcggggag acgctatggc tttttcagaa ccgcgaccgt aagcgagcgg caaaccccca
     1021 tttcacaacc gcgaccgtac ttgtcctgag tagccttggc gaagcaggaa gggagcggcc
     1081 cccgtgtggt gttggccact ttgcggcccg gcctgtcctt gaccgacctg caaaggtcgg
     1141 tcaagggccc ctcgctcacc tggctgcgac aggcgtcgcc gtggcgagcg ctcggggttc
     1201 tgaaagggcc gcccagacta tcacgtaccc cttcctgacg cgggcacatc ggctgccgcc
     1261 gaggcggaaa tcccaaacac acatggtgga gtcgagagtg aggaggggga tctttcagtc
     1321 gacggcgtcg gcagcttcac tcttgcggcg ctcgacaatg cgggctcggc cgttgccgtt
     1381 ctggccgtta ccggcagctc cgttaccgtt gccgctgggt gcccgagtcc gggagcgcgg
     1441 cgacgttttc ggggcgttga ggttctcaaa gaagttgccc atgctccgcc atcgctcata
     1501 tcggcgcttg agcagggtgt ccaggcgaac gcgcttcaac tcgcggaggg tctcgctgac
     1561 atattgctcg aaggtggcga tcatggctga gggattacga tggccgccgc cgagcggctc
     1621 aggcaggatc ttgtcgatca ggtcgagttt cttgaggtct tgggcggtga gcttaaggtg
     1681 ccgggcggcc tcggccacct gttcggtgct cttccagaga atggccgcgc aaccttcggg
     1741 cgtgatcacg gagtagtagg cgtgctcgaa catcgccagc cgatcggtca cgccgatgcc
     1801 cagcgccccg ccgctgccgc cctcaccgat gaccaccccg acgatgggca cgcgcaggcg
     1861 ggacatttcc atgaggttga cggcgatagc ctgggcgatt ccgcgttcct cggccttgtc
     1921 gcccggatag gcgccctggg tgtcaatgac ggttacgacc gggagcctga atttctcggc
     1981 cagcttcatt ttccggagag ccttgcgata tccctccggg tgggggcaac cgaaattccg
     2041 ttcaatcttc tctttggtct cgcgaccctt gtcgtgcccg acgatcatca ccttctggcc
     2101 cccgatccgg ccgaagccgg tgacaatcgc cttgtcgttt ccgaaacagc gatccccgct
     2161 cagttcacag aagtccttga cacagccctc gatatagtct cgcagcccgg gccggcgggg
     2221 gtgtcgggca accaggacgg tttcccacgg ggtcagccgg gcataggtcc ggcgcatcat
     2281 gctctcgagc gtagtccgca gttctttcag ctcagcggaa agatcccgct gggtggtgtt
     2341 ttgaacggcc tccagctcgg cgatctcccg ctcgatccgc aacaggggtt tctcaaattc
     2401 cagcatgacc cccgggatgg agagcatatc accggctgga cgtactccgg acagtgtgct
     2461 catggtatct ttctgctcag tctcacggcc cagacttcgc cgcagtccgc gccatctgca
     2521 gtcgttgtcc cgggcacaag tggtatagga tatcacgtcg gctcgatgaa gcaagggtca
     2581 tttgacgcaa cccccacccc ccggctacag taggcgcact tggaggcacc ccatagtcgt
     2641 gcacaagatc attgatataa agaatttaga ccaaatcgcg atcatcggag taggtttatt
     2701 gggtgggtcg attgggttgg ctctgcgcgc cgcaggattt ggcggatgtc gtattggagc
     2761 cggacgccgg gcttcctccc tgcggaaagc cctcaattgc gatgccgtgg acgtcgtgac
     2821 ccgggacgtc gccaaggcag tttccggctc ccaactggtc attctgtgta cgcccatccg
     2881 tcagttcgat ggtctgataa cccgaatggc cccggcgctg tcaccgggaa cctacgttac
     2941 ggacgttgcc agcacgaaag cccaggtcgt ccggatggcc gagcgcctct tgcccgaccc
     3001 ggttcgcttt gtcggttccc atcccatggc aggttcagag aagacgggtg ttgagttcgc
     3061 ccgcgcggat ttgtttcaat tgtcgacctg catccttacc ccgacttccc gaacgaagcc
     3121 ggcgaccgtc agttggatgc gcaggttttg ggaggcgctg gggtgtcgaa cggtggtgac
     3181 cagccccgaa cggcacgatc ggttgttggc ccgggtcagc catctgccgc acgctgttgc
     3241 cgccgctctg gtcaatctgg cggtttgcga atcggccatc gatctcgcgg gcccggggtt
     3301 tgcggacgcg accagaatcg ccggcgggga tgcgactatg tggacggata ttttcaggac
     3361 caaccggcgg gccgtgctcc aggcgatcga tcggctggtt gccgagctgc gatggttccg
     3421 ccagaagctc gatgacgatg atgcccaggc cattctgaaa tggctggccg ccggtcaaga
     3481 agcccgcaac aaatggatat cgagacgtta caggaagaag gtattacctc catgagtcaa
     3541 cacaccgcct cacctctatg gcggatcacg gtttcaccca agcgagcgga actcgactcc
     3601 cacggggcgg ccatcctccg cgacattcac gaactgggtc tgccgcatgt ccgggtggtg
     3661 gcgtccagcc ggctttttct gatggagacg gatcttccgg agaccgtcct cgaacggatt
     3721 gcctctttgt tgctggcgga cccgatcacc gaatcgtatc gcctgcatcg cggcggcgac
     3781 gagcgggaac cggcccatga cgattctgac tgcgagatcg aggtgcatct caagggaggc
     3841 gtcatggatc cggtggcggc gtcgactctg gcggcggtgg ccgacatggg catcgacacc
     3901 cgcgcactgg cgatttcaac cgcgaggctg tatcgcctgc aggggcttgc gaacctcgac
     3961 gagcttgagt tcgttgcccg gcggttgttc tacaacgact gcatcgaaga ggcgtatctc
     4021 agcgggttcg gacgccatga cgtccggcca cagcatctgc cggtcgcgcc gaaacgcgat
     4081 ttcgaattgc gttacgtgcc gttgcgccgg cttgacgacg tggccttgcg gaagctttcg
     4141 cgcgaggcgc atctcttttt atcgctcgaa gaaatgaaga ccatccagga tcactatcgc
     4201 acgctcggcc gtgatccgac ggacctcgag ttggagatgt tcgcccagac ctggagcgag
     4261 cactgcgtcc acaagaccct caagagcgcg gttcggtatc gcggcgcggc gttcccgaag
     4321 acgtcgtcgg cctcacagaa gatgctgccg gacggcacgg tcgaggtgtg ttatgagaat
     4381 ctgctgcgcg acaccgttgc ccgggcaacc aacgacctga ccggcagcgg acgggtcaac
     4441 tggtgcctgt cggtgttcaa ggacaacgcg ggggtgatcg aattcgacaa ggattacgcg
     4501 gtcgcgttca aggtcgagac gcacaaccac ccgtcggcca tcgagcctta cggcggagcc
     4561 gccaccggta tcggcgggtg catccgcgat gtgatgggtg tcgggctggg ggccaagccg
     4621 atcgccaaca cggatgtctt ctgcgtcggt cggccggact tccccatgaa cctgctgccc
     4681 aagggggtgc tgcacccgcg tcgaattctc aaaggcgtcg tggccggcgt tcgcgactac
     4741 ggcaaccgta tgggcattcc gaccgtcaac ggcgcggtcg ttttcgacaa tcgatatctc
     4801 ggcaacccgc tggtgttttg cggttgcgtg ggattgatgc ccaaccggtg tattgaaaag
     4861 aagccgaacc cgggagacct gatcgtgctg atcggcgggc gtaccgggcg cgacggcatt
     4921 cacggcgcga cgttctcatc gtcggagttg acggatacac acgccgacga gttttcacac
     4981 gccgttcaga tcggcgacgc catcactgag aagaaggtgc tcgacgcgat gatgcgggcc
     5041 cgcgaccacg ccgacggatg cctctacacg gcgacgaccg attgcggggc gggcggcctg
     5101 agcagcgcgg tcggcgaaat gggcgagaag ctcggagctg acgtcgagct atcgaaggtg
     5161 ccgctcaagt acgccggcct gcgatatgac gagatctgga tcagcgaggc ccaggagcgg
     5221 atggtgctgg ccgttccgcc gggcaactgg gagaaggtgc gagcgatttg cgaggcggag
     5281 aacgtcgagg cgacagtcat cggcacattc cgcaacgacg ggcggctgat cattaagtat
     5341 gacggcaaac aggtcggcga catcgatgcc cggttcctgc acggtggtgt tccgaagacc
     5401 cgcagggaag cgacgtggaa acccgcggta tcaggcgcga cccccctcga tccccccttg
     5461 gcaagggggg aaggagtgct gggtgccatg cccacggctc cgcgtgggca tgccaacatg
     5521 ccgggtgggc gagacctgct cctgagccgt ctgtcggatc tcaacaccgc cagcaaggaa
     5581 tggatcatcc gtcagtacga tcacgaggtc cagggagcca gcgtggtcaa gtcgttggtg
     5641 ggccccggcc tggggccgtc ggacgcggcg gtattgcggc cgaggctgtc cagcgacaaa
     5701 ggcattgttc tggccaacgg catctgcccg tcgctgagtg acgtcgaccc ctattggatg
     5761 gcggttgcgg ccatcgacga gtcgctccgc aacgtgattt gcgtcggcgg cgatccgaac
     5821 cgcacggcca tcctcgacaa cttctgctgg ggcaactgcg aggatccgca gacgatgggc
     5881 gcgctcgtcc gggcctgcca gggatgctac gacgcggcgg tggccttcgg tacgccgttc
     5941 atctccggca aagactcgct caacaacgag ttcgctctcg atgccaacga cgccaggcag
     6001 cttggcgaga tgatgccgct gttcaacaac cgcattcgca ttcccgagac gctgctgatc
     6061 agtgcgatga gcgtgatcga cgatgtcaaa cgatgcgtga ccatggacgc caagcccagt
     6121 gaccagccca cgctgttctt ctacgtcggg ctgaaggccc ggcagtggtc ggaggcgctg
     6181 atcaaggacg ccgccggtct gcacgcggcc gtcagcgaac tcattcgatc gggcaaggtc
     6241 ctggccgccc acgactgcag cgacggcggc atcctgcagg ccctggccga gatggccttt
     6301 gccggccagt gcggcatcga ggcgacgttt accgatctgt gtccgttttc gcccggtacc
     6361 agcgggtaca ttctgcaagt gcctgagtcg gcggcgaatg atgttgcccg aatcgccgag
     6421 cagcgcgcgg gcgtttttgc ccgcaggatc gcgacgacgg ctcgcaacga aaagctgatt
     6481 tggagggcag gcgatcgccg gagtgaggtg ccgctggccg atctggccaa ggcgtggcgg
     6541 gcgagcttga attggtaaca gcgagccctg cggtttggcg gcgagccccc ggtttatgcg
     6601 gggggtattc atctgagaac cgctggtgcg agaatccctt ctcgcaccac ccctttggcg
     6661 aatcttttcg cctctggtgg cggcggatga gaatccgcac tcgaagagga ccgagatggg
     6721 aatctcggtc cagccggttt cgggcgatca tgcccacgca gaaccgtggg catggcaccc
     6781 gaccttggaa aggatgcgac tcatgtcttg gatggtcata ctgatggcgt cacttctgca
     6841 ggcggcagcg ggtcagtcgc aggaggcgac gtttgttgaa atcaacggcg agaagtacgg
     6901 ggcgcaggca gatgatttcg gtcccattgg tggcgggcgc gggtacaagc aaatcatcgg
     6961 caagggtgat ttgaccgtca agaacctgga ccaactgatc gacgccctcg gcaaggccaa
     7021 ggccgggcag gtcgtattca tcccgggcga aacggagatc gactgcactg cccgggtgta
     7081 catcgaggaa ctggttctgc aagtccccgc cggtgtcacg ctggcgggca atcgcgggga
     7141 gaacggctcg gtgggagccc tgctctgctc cgatgccctc aagacaccgc tactcatccg
     7201 ggcagccggc cccgacgtgc gggtcaccgg gctgcggatc cgcggcccca accccaagcg
     7261 ataccttgac catcaccacc gatcgttcgc cgagggccgc gggcacgagt actactacaa
     7321 gttcccgacc tccgccggca tcggcaccga gcaccctggc ctggaggtcg acaactgcga
     7381 gatttcgggt ttcagccatt gcggcgtcaa gctttcgcgc ggcgaccggc accatattca
     7441 ccactgcttc attcaccatt gccagtacaa cggactgggc tacggcgtta gccacgatac
     7501 cgcttcgtcg ctgatcgagc ggaacctgtt tgacttcaac cgccactcca ttgccggtac
     7561 cggccggccg gggtgcagct acgaagccag gcacaacgtc gaactgggca cctcgctcag
     7621 ccactgcttc gacatgcacg gcgggcgcga ccgcaaggac ggcacggaca tcgccggcac
     7681 gagaatcgcc atccaccaca acaccttccg cgcgcccgaa cacgcggtgg ctatccgcgg
     7741 cgtgccacag gaaacgtgcg aggtgttcgg caactggttc ccgcgccacg acagtccgca
     7801 ggcggcggtt cgagcgtcgg ccggtactca cgtccgcaac aacgcctatg gcgcggcccc
     7861 cgccgttatg gatccgcagc cgcaggccag agagaaggca gccgccacac agccgggctg
     7921 accccagcgt tgtacgatga ctcactccgg gcacagttct gcaattcggc gttctgtctt
     7981 tgcctgttcg agcccctatt gcccaaccac aatcaccagc ggctggccgg tcgtggcggg
     8041 gtgcgtcaca tggatcgtgt cgacgaactg agcatccgtc agggtcaggt ggtacgttcc
     8101 gggctcgggc gagatggtca gtgacagcgt ggccaggact gtctgaccgg cgggtagcgg
     8161 ttctcttgtc acgccgccga tgccggccgt ggttgtgaac cactgggctg actcctgcga
     8221 ctcgatcgga ggctcggccg ttggggctgc gatggggccg ttgacggccg tcaggtcctg
     8281 cgtcagcgcc cagacggtcg gcggatcgac cccgagcagg gagtccacat ccatgcccca
     8341 cgccgtgacg tccgagccgt caggcgtgag acgcacccat agccagtcca tcaccgtcag
     8401 gtcgtagtag ctggccagag tcgtcggggg ctggcttgcg gcctcttcgg ccgatgttac
     8461 tgtctggccg taggacatga ggacgttgtc cagttcggtc cagtcggccg agtcgatcga
     8521 gaccacattg gcc
//
LOCUS       DAOTPW010000249         8531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7486_length_8531_cov_4.294714, whole genome shotgun sequence.
ACCESSION   DAOTPW010000249 DAOTPW010000000
VERSION     DAOTPW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7486_length_8531_cov_4.294714"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="P5316_18085"
     CDS             <1..1571
                     /locus_tag="P5316_18085"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HRR86923.1"
                     /translation="QQTFARPASLGDEAVLYLEAVAMPETHPAEPEERSRGLAIENGW
                     FVHGDRIVWGYAQHNGWWRAGRRANITRRAPGAIGPNRTEDLDKLTDNMLRYGYPGFE
                     HNFGLWYDRRRDQHDTTRREDGNVVAPFLEQPWARGESGRAWDGLPEYDLTRYNAWYF
                     DRLHEFASLCDRKGTIMFHNFYMQHALLETDAHYVDFPWRPVNCLQDTGLPDRIPAAN
                     AFYDITHPLRRELHRAYIRKCLDVLGDHTNVVHLLSQEYTGPASFARFWLDVVGQWQQ
                     EKGKKVLLGLGATKDVLDELAGDPRVSLLDLRYWWYQPDGSLYAPVSGTEVPGRYVSG
                     TAAARTTPFAIYRQVREYRTRFPQKALIHQIDASRQQTWAFLMGGGSMLIRYLEYADG
                     RDPANYEAPADSATVQPTYDFINARPAHRLQRMVPHDSLVSNPERNFCLADKGRAYLV
                     YLLAGGTVELDLGHTSGGFYAQWFDPRNGDLRDVTPTPVVKGGGMVTFAAPTTDDWVL
                     WLSEVSAFEPDEKR"
     gene            1795..2865
                     /locus_tag="P5316_18090"
     CDS             1795..2865
                     /locus_tag="P5316_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRR86924.1"
                     /translation="MKCRHFLILLWSIIAVFETQRCGSVLAAATQSPPPAAESEARGV
                     RLIFDTDMGNDIDDALALGVIHALQSRGECELLAVTLSKDNEFCAPFVDLVNTFYGRG
                     DIPIGVVRNGKTPEDGKYIRPVAEAADGGRPRYPHDLLSGRQAPQAVAVLRQVLATQP
                     DHSVVVVVVGFSTNLARLLESEPDEHSPLPGTDLVARKCRLLSSMAGIFSKGEKHKEY
                     NVVTDIPAARKVFAEWPTPIVVSGFEIGRAITYPAVSIERDFAYVPHHPLAEGYRLYM
                     KMPYDRPTWDLTSVLYAVRPDRGYFGLSEPGTISIDEQGFSSHAPSAGGRHRYLTVDD
                     GQIIRVREALVQLASQPPTARG"
     gene            complement(3188..3346)
                     /locus_tag="P5316_18095"
     CDS             complement(3188..3346)
                     /locus_tag="P5316_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86925.1"
                     /translation="MKSRTNMWMASLAAFLLGMPLAERAVADDTEEIVFASIGLVWEI
                     IDAAVSAS"
     gene            3782..5005
                     /locus_tag="P5316_18100"
     CDS             3782..5005
                     /locus_tag="P5316_18100"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRR86926.1"
                     /translation="MDTTLSASRPDRDRIFSLDVFRGLTILTMVFVNDVGHLQNIPAW
                     MKHAPENSNSMTFVDLVFPAFLFIMGMSIPLAIERRLARGESWPRLIGHLLLRVVGLL
                     VIGVFMVNIPRYRADLVGLSRSAWVLLLFLGVILVWNRYPPAAGRIRWLFRAFRLAGI
                     ALLVVLAVFYRGGDGQEGTWMRTSWWGILGLIGWAYLIAVGTFLLSGGRTAALVGAVG
                     LLTAVFVGDKTGALGFLGRLHEYINVGGHWGGHGSIAVAGAVLGTLFLSDSAAQRPGR
                     RVLRMLVMGLSLLAGGFLFRPLYGISKVQATPTWCLYSAAICCFVFALLYWLVDVKGI
                     RRWSFFLAPAGRDPLLAFILPSIVASLLALLGIEYLNTHLNKGLPGIIRSAVFALLIV
                     ALTDLLYRLRIRLHL"
     gene            complement(5219..7153)
                     /locus_tag="P5316_18105"
     CDS             complement(5219..7153)
                     /locus_tag="P5316_18105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR86927.1"
                     /translation="MISIIVAVVLLGVLQGMLRIGYARGPGESLLVGSIAAAGCLLCC
                     LLIVPLIERMVEARLTGIRESIRRISDEDVAGKRLPVSGDDEFAALACAVNAVLDRLD
                     AVGNELKTDESRYQSLLNNLPIGFCRVSAEPEFHFVGLNTALVDMLGAASPENVMHVR
                     PIDLFARKRDAVRLWVRLRRQGKVRQVELPLMRTDGSVVWARVSARMVPSHQTASHEI
                     EAFVENITNQKEAEAALEHAERASRAKSESLANISHEIRTPLTSILGYAELLMDADQS
                     PRERLNCLHTIRRNGEHLLSILNDILDLSKIEAGRMLLDVIECSPQQIVSEVGLLLGR
                     RVTDKGLSFETSYRGPIPKTMRTDPTRLRQVLINLVGNAIKFTRSGTVRIEVAMATAP
                     DAPDPRLSFSVIDTGIGMSSEQLQRLFQPFVQAEVSTTRHYGGTGLGLTISKQLVEAL
                     GGEIAVRSTLGKGSTFTVTVPTGPLDGIDMLEIDAPPAESENVSGEPPVPEALRLSGR
                     VLLAEDSRDSQHLVAGLLTKVGLSVTACDNGKVACDRALEALAAGEPFDIILMDMHMP
                     VMNGYEATAYLRQKGYTGAIIALTASAMKSDWEECIRVGCNDFLSKPIERSRLNRILA
                     RYTASGPAGLDASDTSSGNH"
     gene            complement(7808..>8531)
                     /locus_tag="P5316_18110"
     CDS             complement(7808..>8531)
                     /locus_tag="P5316_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86928.1"
                     /translation="RDGRVYVECSGTALGQPATGRIAITFCCDAGTGFETHMVSPSRS
                     GVPQTTMGDDFLLFSRPARGQADLLVAPFTTPLSSSSDNTPDSRACGTWVVAAEAQRF
                     AFASLIRVWPPDIDSPELAAPMVADYRHPLPLQIDVGQLVRTDPGDLDNDGFSEGRGY
                     YVLQLDGAIAKIRIDGHRKLRFAPLFKLVDVANRDVWIYVDGRQIRDTVQDEDGNLIF
                     AVPGVISRETLVEITSRPIDAG"
BASE COUNT         1582 a         2677 c         2559 g         1713 t
ORIGIN      
        1 gtcaacagac gtttgcccgg ccggcgtccc ttggcgacga ggccgtctta tacctcgagg
       61 ccgtggcgat gccagagact caccctgctg aacctgaaga acgatcccgc ggacttgcca
      121 tcgagaacgg atggttcgtt cacggagatc gcatcgtgtg gggctacgcc cagcacaacg
      181 gctggtggcg cgccggccgg cgagccaata tcactcgccg cgcacccggt gccatcggcc
      241 cgaaccgaac cgaggatctg gacaagctca cggacaatat gctgcgctac ggctatccgg
      301 ggttcgagca taacttcggg ctgtggtacg accgccggcg cgatcagcac gataccaccc
      361 gtcgggaaga cggaaatgtc gttgctccgt ttcttgaaca gccatgggca cgcggcgaga
      421 gcggaagagc ctgggacgga ctgccggaat acgatctgac acgatacaac gcctggtatt
      481 tcgaccgcct ccacgagttc gcctcccttt gtgaccgcaa agggacgatc atgtttcata
      541 acttctacat gcagcatgcc cttctggaga ccgacgcgca ttacgttgac tttccatggc
      601 ggccggtcaa ctgccttcag gacaccggtt tgcccgaccg tattccggcc gcgaacgcct
      661 tctacgatat aacccatcca ctccgacggg agctgcatcg cgcttacatt cgcaagtgcc
      721 tggatgtact gggtgatcac accaacgtgg tgcatttgct gagtcaggag tataccggcc
      781 ccgcgtcctt cgcccggttc tggctcgatg tcgtcggaca atggcagcag gaaaagggca
      841 agaaggtcct gctcggcctg ggggcaacga aggatgtgct ggatgaactc gccggcgacc
      901 cgcgggtgtc cctgctggac ctgcgctatt ggtggtacca gccggatgga tctttgtacg
      961 cccccgtgag cggcaccgag gtgcctggca gatacgtctc aggaaccgcc gcagcccgta
     1021 ccacgccgtt cgcaatctac cgccaggtcc gcgagtaccg cacgcggttc ccccaaaagg
     1081 ctttgattca tcagatcgac gcatcgaggc aacagacttg ggccttcctc atggggggcg
     1141 gctcgatgct cattcgctac ctggaatatg ccgatggaag ggaccccgca aactacgagg
     1201 cacccgcaga ttcggcaact gtccagccaa cgtacgactt catcaacgca cggccggctc
     1261 atcgcctgca gagaatggtg cctcatgact cattggttag taacccggag cgcaacttct
     1321 gcttggcgga caagggcagg gcgtacctcg tctacttgtt ggccggcggc accgtggaac
     1381 tcgacttggg gcacacatcg ggcggcttct atgcacaatg gttcgacccc agaaacggcg
     1441 acttgcgcga tgtgactccg actcccgttg tcaagggggg cgggatggtc acgttcgcag
     1501 ccccgacgac ggatgactgg gtcctttggc tgagtgaggt gtcggctttc gaaccggacg
     1561 agaagaggtg agacgctgct tcgtaagtcg acgtgtccat gatgtattga aagaaaggat
     1621 gtgtccgatg atttgtggga tccaccgatt ggttggactt gtgatctcgt cttggtacag
     1681 ttgatgattg agcagagccc ggcatgcaag cttcctgcat gccgttgagg cctctcatct
     1741 gcccgacagc gcctgccggg cgagaaaacg cgattgcaca ggaggccgac aaccatgaaa
     1801 tgccgccatt tcctgatact gttgtggtcc atcatcgccg tttttgagac gcagagatgc
     1861 ggctccgtat tggccgctgc gacgcagtcg cccccgccgg ccgcggaaag cgaggctcga
     1921 ggggtacgcc tcatcttcga caccgacatg ggtaatgaca tcgacgatgc tcttgccctc
     1981 ggcgtcatcc atgccctgca gagccggggc gagtgcgagc tgctggccgt taccctgagc
     2041 aaagacaatg aattctgcgc gccctttgta gatctggtca atacctttta cgggcgcggc
     2101 gacataccca tcggagtcgt ccgcaacggc aagacgccgg aagacggcaa atacatccgg
     2161 ccggttgccg aggcggcaga tgggggaagg ccgcgatacc cccatgatct gctcagcggc
     2221 aggcaggcgc cgcaggccgt ggccgtgctc cggcaggtct tggccacgca gcccgatcac
     2281 tctgtcgtgg tggtcgtggt tggcttctcg acgaatctgg cccgcctgct ggagtccgag
     2341 ccggacgagc attctccact cccgggcact gaccttgtgg ctcgcaagtg ccgcctgctg
     2401 tcaagcatgg ccggcatctt cagcaaaggc gagaagcaca aggaatacaa cgttgtgacg
     2461 gatatcccgg cagccaggaa ggttttcgct gaatggccca cgcccatcgt ggtcagcgga
     2521 ttcgaaatcg ggcgggcaat tacctatccg gccgtcagca tcgaacggga ttttgcctac
     2581 gtcccgcatc accccctggc cgaaggttac cgcctctaca tgaaaatgcc ctatgaccgc
     2641 cccacgtggg atctgaccag cgtcctgtat gcggttcgac cggatcgggg atacttcggg
     2701 ttgtcggagc ccggaacgat ctccatcgac gagcaggggt tctcgtcgca tgctcccagc
     2761 gccgggggaa ggcaccgata cctgaccgtc gatgacggcc agatcattcg tgtgcgcgaa
     2821 gcccttgtgc aattggctag ccagcccccg acggcgcgcg gataggcagg cgccgccgcc
     2881 gtgtcgcctg tcataacggt ttcggatctc aaacctgtcg ggaacagata agacaggctg
     2941 aaaccttaag gccggaaatc cgtgcggctt gcgcgcgttt cgggatgaca acggggcctg
     3001 gttgcggccc gtaaaaaggg ccgcccgcct tggccatctt ccagcgcaca ggttattcgc
     3061 gtccaccgcg attggataaa caccgccaat cgcggtgggc ctgggcgcac aacaagagcc
     3121 tttcgcggcc caatggtgtt acgatcgcgc tgcttcaatc cccgctcgcc gatcagcgtc
     3181 gggtgactca gctcgctgaa accgcggcgt caatgatttc ccaaacgagc ccgatcgacg
     3241 cgaaaacgat ctcttccgta tcgtcggcga cagccctttc tgccaggggc atgcccagca
     3301 gaaacgccgc aaggctggcc atccacatgt ttgtccttga tttcatacac gactcctttc
     3361 gcaatcgcct cgccgcgccg tcttccatgc cgaggacgtc ctccggcata cgattcgcta
     3421 ccgtcgatgc cagactctcg gcgcgggtcg tatccccatg cttactatga ttgcagtaac
     3481 agcgtctgtc aaaacctgtg ccggcggcac tcacgcgtgg gcggccaaac acccgcccga
     3541 tctcgttcat atgaagatcc caatagcggc ttcaaacgtc cgcgttgttg cgggtagccg
     3601 cgctcccctg ttgtacgacg gcctgcaaca aggcccccct tatttatcga aaaatcgccc
     3661 gtcgagactt cagtggtagg atccgtttcg gcataccgcc cggtcgtcgc ccgaagctcg
     3721 caaaccatgc ggaacattgt tatgatcccg cccggcccga caggtgatga gagcaaagct
     3781 agtggacacc actttgagtg cctccagacc cgaccgcgac cggatctttt ccctcgatgt
     3841 gttccggggc ctcaccattc tgaccatggt gttcgtgaat gacgtcggac atctgcagaa
     3901 catccccgcc tggatgaagc acgcgccgga gaattccaac tccatgacct ttgtcgattt
     3961 ggtcttcccg gccttcctct tcatcatggg catgtccatc ccgcttgcga tcgaacggcg
     4021 tctcgctcgc ggcgagtcct ggccgcggct tatcggccac ctgttgctgc gcgtcgttgg
     4081 cctgctggtg atcggcgtct tcatggtcaa tattcctcgc taccgcgcgg atctcgtcgg
     4141 gctcagcaga tcggcgtggg tgctgctgct gttcctgggc gtcatcctcg tatggaaccg
     4201 ctacccccca gccgccgggc gcattcgatg gctttttcgc gcctttcgct tggccggaat
     4261 cgctcttctt gttgttctgg ccgtgttcta ccgtggcggt gacggccagg aaggcacgtg
     4321 gatgcggaca agctggtggg gaatcctcgg cctgatcggc tgggcctacc tcattgccgt
     4381 gggcacgttt ctgctgtccg gcggtcggac ggccgcactg gtgggcgcgg tcgggctgct
     4441 caccgcagtc tttgtgggcg acaaaaccgg agccctggga ttccttggcc gcctccacga
     4501 atacataaat gtcggcggtc actggggtgg ccacgggtcg atcgccgtgg caggcgcggt
     4561 cttagggacg ctctttctat ccgactcggc tgctcagcgg cccggccggc gcgttctgcg
     4621 gatgctcgtg atgggtttga gcttgctggc gggcggtttt ctgtttcggc cgctttacgg
     4681 catttcgaaa gtccaggcaa cacccacgtg gtgcctgtac agcgcggcaa tctgctgctt
     4741 cgtgttcgcc ctcttgtact ggctcgtcga tgtgaagggc atccgtcgct ggtcgttctt
     4801 cctcgccccg gccggccggg acccgttgct cgccttcatc ctgccttcga tcgtggcctc
     4861 gctgctggct ctccttggta tagagtacct caacacccac ttgaacaagg gcttgcccgg
     4921 cattattcgc tctgcggtat ttgccctctt gatcgtcgct cttaccgacc tgttatatcg
     4981 acttcgtatt cgactgcatc tctgagccga cacgcggcat ccccgcgtca cggtgccggg
     5041 gcaatagaga ctgcctcaat gagccggacc ggtgcgttgc agcaagtgcc cgaatgctcc
     5101 catcagcgtc cgtggtggcg atcggcttcg ctctcacctg tcgatggcct tgaggcggca
     5161 caaacgctga ggggggcgaa acgagtcgag tccctcacac cgagacagga cacccccgtc
     5221 agtggttacc ggacgatgtg tcggaagcgt ccagcccggc ggggcctgac gccgtgtaac
     5281 gggccagaat acgattcaaa cgacttcgct cgatcggttt ggaaaggaag tcattgcagc
     5341 cgacccgaat gcactcttcc caatcggact tcatcgcgct ggcggtcagg gcgatgatcg
     5401 cgccggtgta gcccttttga cgcagataag ccgtggcctc gtagccgttc atcacgggca
     5461 tgtgcatgtc catcaggatg atgtcgaagg gctcgccggc cgccagcgct tccaacgccc
     5521 gatcacaggc gactttgcca ttgtcgcacg ccgttaccga caggccgacc ttcgtgagca
     5581 gtccggcgac gagatgctgg ctgtcccggc tgtcctccgc cagcagcacc cgtccgctca
     5641 gtcgcaatgc ttccggaacg ggcggctccc cggatacgtt ctcggattcc gcaggcggtg
     5701 catcgatctc cagcatgtcg atgccgtcca gcggaccggt gggtactgtg accgtgaaag
     5761 tgctgccctt gcccagcgtg ctccggaccg cgatctcgcc gccgagagcc tcgaccagtt
     5821 gttttgaaat ggtcagcccc agcccggttc cgccgtaatg acgtgtcgtc gagacttctg
     5881 cctgaacaaa aggttggaag agcctctgca actgttcgga tgacatgcca atgccggtgt
     5941 cgatcaccga aaatgacaat cgcgggtcag gggcgtccgg agccgtcgcc attgccacct
     6001 cgatccggac tgtgcccgat cgtgtgaatt tgatcgcgtt gccgaccaag ttgatcagga
     6061 cctggcggag tcgcgtggga tcggtccgca tggtcttggg aatcgggcct ctgtaagagg
     6121 tttcgaacga gagaccttta tcggtgactc gtcgtccgag caacaggccg acctcgctta
     6181 cgatctgctg cggcgaacac tcgatgacat ccaataacat gcgaccggcc tcgattttgg
     6241 acaagtccag gatgtcgttc aggatgctga gcagatgctc gccgtttcgg cgaatcgtat
     6301 gcaggcagtt cagccgttcg cgcggcgatt ggtcggcgtc catcagcagc tcggcatagc
     6361 cgagaatgga tgtcaaaggt gtgcggattt cgtgactgat gttggccagg gattcgctct
     6421 tggcacggct ggcccgctcc gcgtgctcga gagccgcctc ggcctccttc tggttcgtga
     6481 tgttctcgac gaaggcctcg atctcgtgcg acgcggtttg gtgggatggg accattcttg
     6541 ctgataccct tgcccacacc acgcttccat ccgtccgcat cagaggcagc tcaacctgcc
     6601 tgaccttccc ctgtcgcctg agacgtaccc acaacctcac ggcgtcccgc ttcctggcaa
     6661 acagatctat cggccgcaca tgcattacgt tctccggcga tgcggcccca agcatatcga
     6721 ccagtgcagt gttgagaccg acaaagtgga actcgggttc tgcgctgact cggcaaaagc
     6781 caatgggcag gttgttcagc aatgactggt accgactctc gtcggtcttg agttcgttgc
     6841 ctaccgcgtc cagcctgtcc agtacagcat tgacggcgca ggcaagtgcc gcgaattcat
     6901 cgtctccgga aaccgggagc cgcttcccgg cgacgtcctc atcactgatc cggcggatgc
     6961 tttcgcgaat gcccgtaagg cgcgcctcga ccatccgctc gatcagcggc acgatcagca
     7021 gacaacaaag caggcagccc gccgccgcaa tcgaaccgac cagcagggat tcacccggcc
     7081 ctcgggcata accaatgcgc agcatcccct ggagcacccc caggagcacg actgcaacga
     7141 taatactgat cacccgtgtc ttgcttcgca acgtcatgca tatggtcgct tacaggcgat
     7201 ctccccaacg agatggtatt cagccgtctc cccatattcg gacagaagga cgaacaagcc
     7261 cgacaaaggt cccaaaaacg gccaaccctt ggcgatgcac cgcacgcggt cgcagcaggc
     7321 gcgggtgcac ctggctcatc cggacggccg ctggcgccct ttcctcgatg tttgctttcg
     7381 ccgaatcagc gggtgccacc ggcggcctgc ccgcccgcgc acaatcggcc caccgaaaac
     7441 gtcggtagaa tcatcggaca gccggtaccg ccggattggc taatggcgat cctgtgccta
     7501 agaaaatctg ctagacagcg gagagacgcg aagacggggg cagcccccag gagtccgcta
     7561 tggcgctccg aggatcgaaa acgccgggct gtctcggtcc aacgtccgat catgtggcgc
     7621 caccgtaggc aggatcagga cttgtccccg ctccgccgcc ggccgccgga tccgcgacag
     7681 taccggcagc tccatcgcgg tctgccctgc ccttgatcga cttgcgggag ggtagtcaac
     7741 ggtttgttgg ttaccctgta acaggcagtt gcgccatccg cttgaacggt ggcagacctt
     7801 gccgcgatca cccggcgtcg atggggcgcg aagtgatttc gaccagcgtt tcgcgcgaga
     7861 tgacgccggg taccgcaaag ataagattac catcctcgtc ttgtacggta tcccggatct
     7921 gacgcccgtc gacatagatc cagacatcgc gattggcgac gtcgaccagc ttgaagagcg
     7981 gtgcaaatcg cagctttcgg tggccgtcga tccgtatctt cgcgatggca ccgtcgagct
     8041 gcaggacata atagccgcgc ccctcgctga aaccgtcatt gtccaggtct cccgggtcgg
     8101 tccggacaag ctgcccgaca tcgatttgaa gggggagggg atggcggtag tcggccacca
     8161 tgggcgcggc cagttcgggc gagtcgatgt ccggcggcca gactcggatc aggctcgcaa
     8221 aggcaaaccg ttgggcttcg gcagccacca cccaggttcc gcaagcacgg gagtcaggcg
     8281 tattgtcgct ggacgacgag aggggagtcg tgaagggtgc cacgagcaga tcggcctggc
     8341 ctctggccgg tctgctgaag agcaggaaat cgtcgcccat tgtcgtctgg ggcacgccgg
     8401 accgggaggg cgagaccatg tgagtctcaa agcccgtgcc ggcgtcacag cagaaagtga
     8461 tcgcgatccg gcccgtggct ggttgcccaa gggccgtccc cgagcattcg acgtagacgc
     8521 ggccatcacg g
//
LOCUS       DAOTPW010000250         8491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7519_length_8491_cov_5.865576, whole genome shotgun sequence.
ACCESSION   DAOTPW010000250 DAOTPW010000000
VERSION     DAOTPW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7519_length_8491_cov_5.865576"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..706
                     /locus_tag="P5316_18115"
     CDS             50..706
                     /locus_tag="P5316_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRR86929.1"
                     /translation="MVRMKAEARREQLLKTAARCFAKYGYRGTTTAKIAAEAGVSEPI
                     IYRHFRNKQELFIALIEKVGDEVMNNWNQAIRNARSPLEKLQSLLYRNPATADPWTAS
                     VYQLLFHASTEVSEPAIQQAIRDHYERYVRTLAAVMSEAQKAGQIRTDLPAEWLAWQI
                     IHAAVGFAMVRPLNIPSHANLKFVQGTIRLLAELLTRNTGGEHLIPEAKPRSGKRLAK
                     "
     gene            918..1616
                     /locus_tag="P5316_18120"
     CDS             918..1616
                     /locus_tag="P5316_18120"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRR86930.1"
                     /translation="MSVGQLPSFSEPVGLFPLPNAVLLPGGTLPLQVFEDRYRAMVRD
                     AIEDQGIIAMALLRPGYEPVYHTNRAEIYPVVCVGRINDHVQIPDGRYFINLLGLCRA
                     RVRQEDRDGEYRIAMLDPMLPTGSGIEVDGEFAARNSLRAVLGLEQIETTDVIQRVRN
                     LVDSELPLGELVDRVASALLPAEMVELKQQLLEEMNPIRRAGILTHELRTLVQVMTVR
                     QKDLDRWPRLGSMN"
     gene            complement(1726..2697)
                     /locus_tag="P5316_18125"
     CDS             complement(1726..2697)
                     /locus_tag="P5316_18125"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRR86931.1"
                     /translation="MQTADSPTGIDLTVRLGRLVLRNPVLTVSGTCGYGDEYAPFMDI
                     SVLGAFTTKSVTLNQRRGNEPPRIVETASGMLNAIGLANVGLERFINEKLPVLSDLDV
                     PVLVNVAGHSIEEYRTVCGRLDPYDAIAGLELNVSCPNVSDGLVFGTDADALSRLVAS
                     VRKVVKRSLLIVKLSPNVTDITKTARAAIDAGADCLSLINTFVGMAIDVERQRPILAN
                     RTGGLSGPAIKPLALNLVSRVYREVAGSANIPLIGMGGIRTWSDAVEFILAGATAVGI
                     GTALFIDPTTPVKVLEGVEAYLRRHRLKSVSELIGRLRFDKNEHPAG"
     gene            2837..3187
                     /locus_tag="P5316_18130"
     CDS             2837..3187
                     /locus_tag="P5316_18130"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRR86932.1"
                     /translation="MRRFMLKSKIHRATLTGTDLNYEGSITIDQDLLEAADILPGEQV
                     HVLNLHNGARLITYAIVAPKGSGTVLLNGPAARLGMPGDIVTILTYCDVSEEEARTFK
                     PRVVLVDDKNRPRK"
     gene            3258..4511
                     /locus_tag="P5316_18135"
     CDS             3258..4511
                     /locus_tag="P5316_18135"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRR86933.1"
                     /translation="MEIDLQRFYRVNRPVLMWIVFFAGLFLLRQFFALVFITFIFAFV
                     MRNVARFLTANTRMPYWAAVVFPYLGALTLLVLLMMTAIPRVVDEAAEFGRKVPGLLE
                     ELAKEVQKSAEYYGMEPMLAKFVHAGTETSTTAPANDGDGMASGPERVNIQELAKTLQ
                     TLAIGLLPGMQVQDAKGNASEPESKGSTRSDDLRASILGLFNRFIRGVISGTLQFLLA
                     ILLSFLIVLDYEAVAGELRKWRTSQVGRFFHEAAASVVDFSRVVGRAFQCQIVVALLN
                     ATITCVGMAILDIQPLLLLTTIVFLFGLIPVLGVFISSVPIILIAFNSHGLTLALIAL
                     GMIVGVHLLEAYVFNPRIYAARFHLNPVIVLIILLVAHELFGVWGMLLGIPVTTYVLN
                     IAQIPALPPPKNRLRRNSATKAPAA"
     gene            complement(5366..6493)
                     /locus_tag="P5316_18140"
     CDS             complement(5366..6493)
                     /locus_tag="P5316_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86934.1"
                     /translation="MLDLDLARLSPEVAKIIMLRGARTPSLQWQEHQNTGAKKTLDAI
                     DDARLFGSGVVINEGMAAATRSLLYLWNGWIGECKMYAQAVPEKERFHIIAFCERMEG
                     NSKSAKECFQRIGESPVFTELRTYALEAISPGAAPEVHRFRKVLEQNEAWEPYAFCDL
                     YMQVLSGQLGTSSASLVTGIQTREFALLFTHCYQAATGTDLKKRATGGPQTQARSPKL
                     LKKRKPPTSRTGAQRAGRLDREPESKLFGKTTGAEKTARPAGSPPVVIQVKVICPSCR
                     EKLTFPVAARGKNHTCPKCHTEFLIPEKKGTGTGTSSSVVPENTEGAGVKILCPRCKS
                     LMVFPDSARGEQRNCSACGTPFIVPIKKTGAEPQAEPLQIT"
     gene            complement(6718..7224)
                     /locus_tag="P5316_18145"
     CDS             complement(6718..7224)
                     /locus_tag="P5316_18145"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRR86935.1"
                     /translation="MMASMDWFGDLYKAVPLPLCGLILVSCAVFCGTLIGLEREHRAK
                     PAGVKTVSLICIGSTIFTIASILIAGEGGADRGRIAAQVVTGIGFLGAGAIIRDHKTI
                     IGLTTGATIWVASAIGVLIGAGYAAGGIAMTVVVLGVLIALRRSEDYFRRRAGQSPSA
                     SCRDDDDA"
     gene            7659..>8491
                     /locus_tag="P5316_18150"
     CDS             7659..>8491
                     /locus_tag="P5316_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR86936.1"
                     /translation="MEGNDAPTVLLVDDCPANRRLFERLLVKDGLVTVHAVNGQEAVE
                     LATQHLPHVIIMDIMMPVMDGLEATRRLRADPRTARIPIIIVSAKTDQEDLTAGLAAG
                     ADEYLFKPIHAREFRLRVRSMVRLREAQLDLERANASLSQQTCILARLNEFSESALVA
                     DSIESNCRHIVETASQLLDSRRVSLLIPDESGEHLRFGYAVGIPPDIWRNQSVPVDSP
                     IAGRVFQTRQEIVVGRQEAPQIARSPYESCYFASLPLICEPLSAGSGVIGVLNITEKA
                     GG"
BASE COUNT         1662 a         2658 c         2408 g         1763 t
ORIGIN      
        1 tccggcattg gcgtctcgtc atgggtcatt cgggcccgga gggccggtaa tggttcggat
       61 gaaagccgag gctcggcgcg agcagttgct caagacggcg gcccggtgct tcgccaagta
      121 cgggtatcgc ggcaccacca ccgccaagat cgcagccgag gccggggtct ccgaaccgat
      181 catctatcgc cactttcgca acaagcagga gctgttcatt gccctgatcg agaaagtcgg
      241 cgacgaggtc atgaataact ggaaccaggc catccgcaat gcccgcagcc cgctggagaa
      301 gctgcaaagt ctgctctacc ggaacccagc cacggctgac ccatggacgg ccagtgtgta
      361 ccagctcctg tttcatgcct cgacggaggt ctccgaaccg gccatccagc aggccatccg
      421 cgatcactac gaacggtatg tccggaccct cgcagcggtg atgtccgaag cccagaaagc
      481 cggccagatc cgaaccgatc tgcccgccga gtggctggct tggcagatca tccacgcggc
      541 agtggggttt gcgatggtgc gaccgctgaa tatccccagc cacgccaatc tcaaattcgt
      601 ccagggcacg attcgcctac tcgccgagct gcttacccgg aacaccggag gtgaacacct
      661 tattcctgag gctaaacctc gctctggcaa gcgcttggca aaataaacaa tgctggcccg
      721 gatgcacttt ctctcgctgt gggttgagac caggcctcag ttcatggcga gattcggctg
      781 gcgtccaccc cagagatcgt gcgccagaaa cccaagcgac atcccgccga gccggaacct
      841 gatgatgctg ttctcccgat gatggtgcct tgaatcacgt ttctgatcga caggccaaga
      901 tgggtataat tcggtgtatg agcgtaggtc agttgccttc cttttctgag cccgtagggc
      961 ttttcccttt gcccaatgcc gtgcttctgc ccggcggcac cctcccgttg caggtgttcg
     1021 aagaccgcta ccgggcgatg gtcagggacg ccattgagga tcaagggatt atcgccatgg
     1081 ctctgttgcg gccgggttac gaacccgtct atcacaccaa cagggccgag atctaccccg
     1141 ttgtctgtgt cggcaggatc aacgatcacg ttcagatccc cgacggccgc tacttcatca
     1201 acctgctggg gctctgccgg gctcgcgtcc gccaggaaga tcgcgacggc gagtaccgca
     1261 tcgccatgct ggaccccatg ctaccgacgg gctcgggaat tgaggtcgac ggcgagtttg
     1321 ccgcccgtaa ctcgctgcga gctgtccttg gccttgagca aattgagacc accgacgtca
     1381 ttcaacgcgt gagaaacctc gttgacagcg aactgccgct gggcgagctg gttgaccggg
     1441 tcgcctcagc tctgctgccg gccgaaatgg tggagctcaa acagcaactg ctggaagaga
     1501 tgaatcctat ccgacgggcc ggaattctta cacatgaact gcgcacgctg gtgcaggtga
     1561 tgaccgtccg tcagaaggat cttgaccgct ggccacggct cggatcgatg aattagacaa
     1621 ggtttgggcc atcggtcctt gacgacgtaa gacgataccg tgacgacgcc gcagtctcgc
     1681 gcctctgctt gctttgtctc cgattctcga tttcccgttc acacttcagc cggcggggtg
     1741 ctcatttttg tcgaacctga gacggccgat cagctcgctg accgacttga gacggtgtcg
     1801 gcgaagatag gcttccacgc cttccaggac tttcacaggt gtggtggggt cgatgaacag
     1861 ggccgtgccg atgccgacgg cggtcgcacc cgccaggatg aactccacgg catcgctcca
     1921 ggtgcgtatg ccacccattc cgatcagagg gatgttcgct gagcccgcca cttcgcgata
     1981 gacgcgactg accagattca gggccagcgg cttgatggcc ggaccgctca gaccgcccgt
     2041 gcggttggcc agaataggtc gctgtctctc gacatcgatg gccatgccga cgaacgtgtt
     2101 gatcagcgag aggcaatccg ctcctgcatc gatcgctgcc cgagccgtct tggtgatgtc
     2161 ggtcacgttg ggtgacagtt tgacgatcag cagggaccgc ttgaccactt tccggacgct
     2221 cgcgaccaag cggctcaatg cgtcggcatc agtgccgaac accaggccgt cggacacgtt
     2281 gggacaactg acgttcaatt cgagaccggc tatcgcatca taggggtcaa gtcgcccgca
     2341 gacggtcctg tactcttcaa tgctatggcc ggctacgttc acaagaacgg gcacatccag
     2401 gtcgctcagg acgggaagct tttcgttgat gaatcgttca aggcccacgt tggccaggcc
     2461 gatggcgttc agcatcccgc tggcggtctc cacgattctg gggggctcat ttccacgacg
     2521 ctggttaagc gtcacgcttt tggtggtgaa ggctccaagg acgctgatgt ccatgaaagg
     2581 ggcatactca tcgccgtaac cacaggtgcc cgagaccgtc aaaaccgggt tccgcagcac
     2641 caggcggccc aggcgcacgg tcagatcgat gccggtcggg gaatcagccg tctgcatggc
     2701 gggggagcat ccggcatccg ctccgggatg tcaagatgac cggctttccc tcccggactc
     2761 gtcgaggtat cataatccgt tcagccatac ctgtatgttg atgcgaccga cgacggctga
     2821 agggaaaaaa cgcgacatgc ggcgtttcat gctgaaatcc aagattcatc gggcaaccct
     2881 gacgggaacc gaccttaact acgagggcag cattaccatt gaccaggacc tgctcgaagc
     2941 ggccgacatc ctgccagggg aacaggtcca cgtgttgaac ctgcacaacg gtgcccggtt
     3001 gatcacctat gcgatcgttg cccctaaagg gtcgggcacg gtgctcttga acggcccggc
     3061 ggcccgcctg ggaatgcccg gagacattgt cacgatcttg acctattgcg acgtctccga
     3121 ggaagaggcc cgtaccttca agcccagggt ggtcctcgtg gacgacaaga accgtccgcg
     3181 caagtgaccg ttgctgacga caaggcgatt ccctatcggg atcggctcgg gagtccgggg
     3241 ctttcggaga aatggccgtg gaaattgacc tccagcgttt ctaccgagtc aaccggcccg
     3301 ttctgatgtg gatcgtcttc ttcgccgggt tgtttctcct ccgccagttc ttcgccctcg
     3361 ttttcatcac cttcattttc gcattcgtga tgcgcaacgt ggccaggttt ctgaccgcca
     3421 acacacgcat gccctattgg gcggccgtgg tgtttcccta cctcggggca ctgaccctcc
     3481 tggttttact gatgatgacg gcgatcccga gagtggtcga cgaggcggcg gagttcggcc
     3541 ggaaagtccc cggactgctc gaagagcttg ccaaggaggt gcagaaatcg gcggagtact
     3601 acggcatgga gcccatgctc gccaagttcg tccatgccgg cactgaaacc tccacgacgg
     3661 caccggccaa cgacggagac ggtatggcgt cgggtccgga gcgcgtgaac attcaggaac
     3721 tggccaagac cctccagacg ttggcgatcg gcttgctgcc cggcatgcaa gttcaggatg
     3781 ccaaggggaa cgcatccgaa cctgaatcaa aaggctccac tcgcagcgac gacctgcgag
     3841 cgagcattct cggcctcttc aatcgcttca tacgaggcgt gatctcgggc acacttcagt
     3901 ttcttctggc catactcttg tcattcctga tcgtgctgga ctatgaggcc gtcgccggcg
     3961 agcttcgcaa gtggcgcaca agccaggtcg gacgattctt tcacgaggct gccgccagcg
     4021 tagtcgactt ctcgcgcgtc gttggtcgag cgttccaatg tcagatcgtg gtggcccttc
     4081 tcaacgccac catcacctgc gtcggaatgg ctatcctgga catccaaccc cttcttctgc
     4141 tgaccaccat cgtgtttctc tttggcctca ttccggtact cggtgtcttt atctcatcgg
     4201 tgccaatcat tctgattgct ttcaacagcc acggtttgac cctggcgctg attgccctag
     4261 gcatgatcgt cggtgtgcat ctgctggaag cctacgtgtt caacccgcga atctatgccg
     4321 cacggtttca cctcaatccg gtcattgtat tgatcattct cctcgtggct cacgagctgt
     4381 tcggcgtctg gggcatgctc ttgggcatac cggtcactac ttatgttctc aacatcgccc
     4441 agatccctgc tcttccacct cccaagaacc ggttacgccg gaacagcgcg acgaaggcgc
     4501 ccgctgcata gcgccgctcg ctgccaacac atgcatcccg caggctgttg acgattattc
     4561 gtcagcagcg cactgcttgg cgaagacaac atcacaggcg taacgcgcct tctttgctgt
     4621 gacatgaggc aagcacataa tcgacgcatc aaactcgacg aacatcatga cacgtttggc
     4681 ctgcgcaatc agtagccgat agcgacagaa gaacgaccga ccgagcggtc cagtcagcgc
     4741 atgaaacggg cggactaccc gatacaaatg gggcaatccg ccctgtttca ttcggccact
     4801 acgaacttat ttcttcttcc gcgcggcctt cttcgccttc ttggctttct ttgcggcttt
     4861 cttcttagcc atggcatact ctcctttcat ggagttgact atcccgtgtg acgcgaacgg
     4921 gattcacgga ttttttaatg cgccttgcgt ccggagtcaa cacctttttt tccgaatcca
     4981 tctttttttt gcccgaacgt cctctgaccc ggtcagcgag tttgggcagg tcgatccgag
     5041 ggggaaccat gctcccgtgc cattcgcatc gattggcatg tgttctccct ccccattttg
     5101 ccgtgatgag attcatcgaa cgatccgagt cggaagttga ataaggatta cgttcttcac
     5161 tcatcggatg cagcccagac cgtcctcgtc aagttcgcat atggacgtcg cggcaggagt
     5221 ctcagatcct gagtctggcc ggcgacgacg atcccggatc gtcggctcca ggcgctgccg
     5281 ccggccgcac gagcaagtcg aaatggacgg atcgagccgc ctgatgatcg gccgctgccg
     5341 acaagaaacc gccaaccggc tccctctagg tgatttggag aggctcagcc tgaggttcag
     5401 cgccggtctt cttgatggga acgatgaagg gtgtgccgca agctgaacaa ttcctttgct
     5461 caccacgcgc cgaatccgga aaaaccatga gactcttgca tcgcgggcag agaatcttca
     5521 ctccagcccc ctctgtgttc tccggaacga ccgaagacga tgttccggtc cctgtgccct
     5581 tcttctcagg gatcaggaac tcggtgtgac acttggggca cgtgtggttt ttgccccgcg
     5641 cggcaaccgg aaaggtaagc ttctcacggc atgagggaca gatgaccttg acctggatca
     5701 cgacgggcgg actgccggcc ggcctggcgg tcttttctgc gcctgttgtt tttccgaaca
     5761 acttgctttc gggttctctg tccagccttc cggcccgctg ggcccctgtt cgagatgtag
     5821 gcggttttcg cttcttcaac agcttcggcg atcgtgcctg tgtctgcggg cccccggtcg
     5881 cccgtttctt gaggtccgtg cctgttgccg cctggtaaca gtgagtgaac agaagcgcaa
     5941 actcgcgtgt ctggatgccc gtgaccagcg aagctgaact ggtgcccagt tggccgctca
     6001 aaacctgcat gtataagtca cagaacgcgt atggctccca tgcctcgttc tgttccagca
     6061 cttttcggaa acggtgcact tccggggcgg cacccgggct gatcgcctcg agcgcatacg
     6121 tccgcaactc tgtgaatacc ggactttcac caattctctg gaaacactct ttggcggatt
     6181 tgctgtttcc ctccattcgt tcgcagaagg ctatgatgtg gaaccgctct ttctccggca
     6241 cggcttgggc gtacatcttg cactcgccga tccagccgtt ccagaggtag agcaggcttc
     6301 gagttgcggc tgccatgcct tcgttgatca cgaccccgga accgaagagg cgggcatcgt
     6361 caatcgcgtc aagcgtcttt ttagccccag tgttctggtg ctcttgccac tgcaacgacg
     6421 gcgtccgcgc gccgcgcagc ataatgatct tggcgacttc cggtgacaat ctggccaggt
     6481 ccagatcgag catcagccca acccagttcg ttcactcgac aaacaaccac gaatccatcc
     6541 tcaccatcga ctcaatgaac ccctggcttt cggctgagga atgacgaatt gtcgcagcgg
     6601 tgttcccaac gagctatggg acgttcgtcg tcgtcaggtt gcaccggctg aacctgcgcc
     6661 gcctcccgat cagagcggcg tgagcgacga cagaccgtgt ttccccgacc ttctgcgtca
     6721 ggcgtcatcg tcatcccgac atgacgcgga aggcgattgc ccggcccggc gcctgaaata
     6781 gtcctcgcta cggcggagcg caatcaggac ccccaggacc acgacggtca tcgcgatgcc
     6841 accggccgcg taacccgctc cgatcaggac accgattgcc gacgccaccc agatcgtggc
     6901 tcccgtcgtc agcccgataa tcgtcttgtg atcgcgaatg atcgcccctg cacccagaaa
     6961 ccctatcccc gtcaccacct gcgcggcgat gcgaccccgg tcggctccgc cctcgcctgc
     7021 gatgaggatg gaggcaatgg tgaagatcgt gctgccaata cagatgaggc tgacggtctt
     7081 aaccccggcc ggcttggcgc gatgttcccg ttccaggccg atcagcgttc cgcagaaaac
     7141 cgcgcacgac acgaggatca gtccgcagag cggcaagggc actgccttgt aaagatctcc
     7201 gaaccaatcc atggaggcca tcatactcga cacgtcgttt ctcccgaggc caagatcaga
     7261 agggtctttt ttggggaaga cacaccatgt ttccgagaac cgtcagctca gccgacgctg
     7321 gccaggagca ttatcggcca cgtccgggta agctgcctgc cgtcaagcat cagtccaaga
     7381 ctccagcagc aacatgagag ttcggtattt acgccggccc ttcatcgaaa cgatacatag
     7441 actgaaggtc ggggacaacc cctgagtaag ttcccgagag ttcgcccccg gccgaacccg
     7501 ggtttgagca agcacaggtc tgccgacaaa cgcacttttg ccggccggtc acccgtgcgg
     7561 tcgactcgaa tccgggcgac aaatccggct gctgtgccgg ccaagtagac atcctcccga
     7621 gcagaccaac ggacggccgg gagaggaacc gtgagcacat ggaagggaac gacgctccta
     7681 cagtactgct ggttgacgac tgccccgcaa accggcgcct gttcgaaagg ctgctggtca
     7741 aggacggtct cgtgaccgtg catgccgtaa acggccagga agccgtcgaa ttagccacgc
     7801 agcatcttcc ccatgtcatc atcatggaca tcatgatgcc ggtgatggat gggctggagg
     7861 caacgcggcg gttgcgcgcg gatcctcgaa ccgcgcggat ccccatcatc atcgtgagcg
     7921 ccaagaccga ccaggaggat ctcactgccg gactggctgc cggggccgat gaatacctgt
     7981 tcaagcccat ccatgcccgc gagttccggc ttcgcgtccg gtccatggtc cggctccgcg
     8041 aggcccagct tgacctcgaa cgggccaacg cctcgctgtc ccaacagacc tgcatcctgg
     8101 ccagactcaa cgaattcagc gagtcggccc tggtcgccga ttcgatcgag tccaactgcc
     8161 ggcacatcgt cgagaccgcc tcccaattac tcgacagccg gcgcgtctcg ctgcttatcc
     8221 ccgacgagtc cggtgagcat ctgcggttcg gctacgccgt tggcatccct ccggacatct
     8281 ggcgaaacca aagcgtgccc gtcgacagtc ccatcgccgg acgagtgttc caaacccgtc
     8341 aagagatcgt tgttggccgc caagaggctc ctcaaatcgc ccgcagcccc tatgagtcct
     8401 gctattttgc cagtttgccc ctgatctgcg agccgctcag cgccggcagc ggggtgatcg
     8461 gggtgctcaa tatcaccgag aaagccggtg g
//
LOCUS       DAOTPW010000251         8422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7588_length_8422_cov_4.981714, whole genome shotgun sequence.
ACCESSION   DAOTPW010000251 DAOTPW010000000
VERSION     DAOTPW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7588_length_8422_cov_4.981714"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="P5316_18155"
     CDS             complement(<1..1140)
                     /locus_tag="P5316_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86937.1"
                     /translation="MQRRLGSCALGITVVLTVRAALAAGPSLIWSQMGSSYRSPDPEA
                     NWGFCPREFNGTLLAPMGDYGACVVKRWTGSDWVDDFLDDNWGTGRDFENLTSLTVYN
                     NKLYMTCETDSNSAYNHGVMVRDSAGNWSVSLPYTRTAEGAMQFGSFGMAVFNGKLYT
                     GVVPYAANTTLTIYEHNGSTWTAIKNYRTGEQLRAWSLGSDGTNLYVGIGTRGRNNAA
                     TLGIERYDGSTWASELAGKAMHSMAFFKGSMYAGGNGRIYKRNPSGVWTEVLNTGQAF
                     VIGMTIIDYGNGMEILYAGTENRPRLYATSDGAAWDMMHEFDTTSAGCYVGQFQNTPV
                     ASVTYFEGSTPKVVKVWRGSPQDASPVITEVSPDPDWATVGTEYIR"
     gene            complement(1158..4073)
                     /locus_tag="P5316_18160"
     CDS             complement(1158..4073)
                     /locus_tag="P5316_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86938.1"
                     /translation="MGRTNTISLCTLIGLLGAPGFPAYAVTIGISADGRFFTLDGVPT
                     YLKGVSYYGAQSITTASYRTSDLDDMVADGFNWIRVWGHWKTPAGEDVSVLTYDGQVR
                     EPYMTRLKTLITECNNRGIIVDVTLNRDAYGTWVGARTQAQHLTCVQTLANQLLSYRN
                     VYIDVSNERDVQDGRYVSLSECGALINAIKAIDPTRLCTASSNPNSQSQLSDFKNIAK
                     MDFICPHLCRSSGCSAQTIGTVNNYCTWMTNLGWRIPIHLQEPFRRGYTTYNPVEDDY
                     LRDCNGGKLAGAAGWCLHNGSNAGSTPPHRSFQMTDGDGRLYAQWDSVEVNVTNWLSP
                     HVAGNDLKVLRYQPEYDEQLAKLIGRRDGSTRSANVAQDSAGYLTYGPYITRLPADSY
                     QATWRMSIDNVTADNEPVVTIDVYTIDTNTILATRTITRQEFTQANVFQDFTLDFAYG
                     TPGNRLEWRTYWHDNSYLKLDYVEVRAQQMPNPPVICEVSPDPDTVIVGQEYTRQLNL
                     CGGYPAPTWSVISGPSGVQVGSTGYVYGWTPTSAQAGQSLTITVRATNSQGHDDESWQ
                     VTVQAPTVPVFEDMLVDTSNISGQYGTGTFSSGGYTLSHSGGYDEFNTSEPRSVDFVW
                     YDRIGTWNVGSIEFDVSGLNPSSGCTKNELMAACDSTGLNPATTGGDYYNSPYQALIR
                     KSYDDTYGNTDRMKMTVVGGNATREERSNVLGWNGGTTYRFRMTWNGSQVRWWRGTPG
                     QTLSEISPPSPQAVGTWSPQKLHIQLGATFRAGPRGVQESGGAPGTCYSLLRIYTQDL
                     GNTPTPPRGGAINAAPQVDAGPDQTITLPAVANLAGTVTDDGLPNPPGQVSVTWTKQS
                     GPGTVTFENANAAVTTASFSQAGTYVLKLTASDSELSGQDTVTITVNPATTPDFDGDA
                     DVDQEDFGLFQACLTGSGQPVETLCAMADLDRDGDVDQADFSRFYECLGGEDLPPGC"
     gene            complement(4196..6661)
                     /locus_tag="P5316_18165"
     CDS             complement(4196..6661)
                     /locus_tag="P5316_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86939.1"
                     /translation="MRGFPGGLVVLLASTSVLASDWTFNLPARQFISGDRLVSASVFH
                     WYTATGGQLSGPWRPVEGRANWTGEPDFWIRQIKDIMDANIDVMYVHLIPSSEQQRVN
                     LFDAMCQLRRQGYEVPKAVPFLDPAITWYGKPFPDVATTAGKDEWVNQYVRYFNQYFS
                     KNPDAQADGYLGIMNGRVMLDTWHMENVVNYSQLTRTDVETRLKAAFGATHPVFNNGV
                     YQIATPWSGLYPSWCDELVWQFSTNSYLVEWAGSPQVRSYTVKPGYWDQNIRNPGFFL
                     ARDGGVHYRDAWQSIIKDSPQYGRVYIESWSEYDEGSGIYAADPGPPYIVPPNPNTDT
                     WSSTANSREYIDTTATFAAQFNRLAERDARFLAHDIPAVMYPGESIDARIVVRNEGNA
                     QWSNAGFYKLGLQGPTLLNCTFDSSANGWTLVRNAFGTSGNTAYENGDWNASYGFSGG
                     GLRTRTGNVDNTAYYNGASTAWARTFHLDRTMNVNIGFRYRLFLPASLESTEAGEARF
                     ELDGQAYGLGGQSYLARFSGGAGTDQDTGWREYSRVFRLSGPADHTVEIGGWNNRKTA
                     ADEFVDVYVDDFVLVDVDGGSPFGPSRFLIDDSDDEIPTYGGIFRGRPKTFAVNLTAP
                     ALGAYTLHFQMLQESVAWFGQSLDVPIEVLALATQPIPSDGAIDVPPSIILRWTAGQT
                     AVGHRLYFGLDSESVGNAEVDSPEFMGLFPIGNESFDPPGLLRQGIAHYWRVDELQES
                     GPVLHGRVWRFTTAVIPGDSDEDGDVDQEDFGYFQACLSGTGQPSGPDCAKADFDGDG
                     DVDQVDFAKFRDCLGGPDQPPGC"
     gene            complement(6985..>8422)
                     /locus_tag="P5316_18170"
     CDS             complement(6985..>8422)
                     /locus_tag="P5316_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86940.1"
                     /translation="GEYLLNRGIQQSDGARPITWEPPVRIIHKSRGTPIHLDLPPGRT
                     SVIELRLQKAIARPPLLPDLAVGRNDVTITGNDVQVTVHNIGSAQAGEFNLILETESG
                     DGWAVVSEVKMAGIPAVTNLEPAIARAVFRNAAPKRGARFRVAVDPEDGIEELYDLNN
                     QIILPAEEVSSTGQQAMPIAPTTLYLTENEGFATCVNRLHPGNRFGYRFHEHTPLKYG
                     PIPQDLQAAGIDPGEVIRLEQDFARRPKVLKYAVQVDGKDWIPQQWTFYMAPTGDGVD
                     LLMVVETKDQGLNEYYGIQQCFRLSGKTNELWRRPIADAPAFSEYDLWNLDEKDKPDK
                     TSLTYVVRQGVWATLPASPEAVGARTPLGVHVDRLLTGGKLEAMPLIGPYKARAQAPI
                     DYGLITRMNREGNWVCAIYWDRTSHVTNHHPADCLHSIVNIGGMPPHAKRAIRGKIYW
                     FEGGLADLLEHWGRDWPDRLHRIEGDSE"
BASE COUNT         1527 a         2531 c         2617 g         1747 t
ORIGIN      
        1 ccggatgtat tcggttccga cggtggccca atccgggtcg ggagacacct cggtgatgac
       61 gggggaagcg tcctgcggcg atccgcgcca gactttcacg accttcggtg tgctgccttc
      121 gaagtaggtc accgaggcca ccggagtgtt ctgaaactgg ccgacgtagc agccggccga
      181 cgtcgtgtcg aactcgtgca tcatgtccca ggctgcaccg tcggaggtcg cgtagaggcg
      241 aggacggttc tcggttccgg cgtacaggat ctccatgccg ttgccgtagt caatgatcgt
      301 catgccgatc acgaacgcct ggccggtgtt gagcacttcg gtccagacgc cggacgggtt
      361 tcgcttgtag attctgccgt tgccgccggc gtacatgctg cccttgaaaa aggccatgga
      421 atgcatggcc ttgccggcca attccgaagc ccaggtgctg ccgtcgtatc gttcgatacc
      481 cagcgtggcc gcattgttgc gtccccgggt cccgatgccg acatagaggt tggtgccgtc
      541 ggaacccagc gaccaggccc gcaactgctc accggtcctg tagttcttga tcgccgtcca
      601 agtcgatccg ttgtgttcat agatggtcag cgtggtgttt gccgcatagg gcaccacacc
      661 tgtgtagagc ttaccattga agacggccat gccgaacgag ccgaattgca tggccccctc
      721 ggccgttcgg gtatagggca gcgagaccga ccagttacct gccgagtctc ggaccattac
      781 gccatggttg taggcactgt tcgagtccgt ctcgcacgtc atatacaact tgttgttgta
      841 gacggtcaga gaagtcaggt tctcgaaatc gcggccggta ccccagttgt cgtcaaggaa
      901 gtcgtcgacc cagtcgctgc ccgtccatcg tttaaccaca caggcaccgt aatctcccat
      961 cggggccagc agcgtgccat tgaactcgcg aggacaaaag ccccagttgg cctccggatc
     1021 gggtgatcgg tagctgctgc ccatttgcga ccaaatcaac gagggtccgg cggcgagcgc
     1081 ggcccgaacg gtcaggacga cggttatccc aagagcacag gatccgagtc gacgttgcat
     1141 cgcacaccat ctcccgatca gcagcccggt ggaagatctt ccccgcccag gcactcataa
     1201 aaccgcgaga aatccgcctg atccacgtct ccgtcgcggt caagatcggc cattgcgcaa
     1261 agggtctcca caggctgtcc cgaccctgtc agacacgcct ggaaaaggcc gaagtcttcc
     1321 tggtccacgt ccgcgtcacc gtcgaaatcg ggagtcgttg cgggattgac cgtgatggtg
     1381 acggtgtctt gaccgctcag ttcggagtcg ctggccgtga gtttcagcac gtacgtgccg
     1441 gcctgactga acgaagcggt cgtgacagcc gcattggcgt tctcgaaggt aacggtgccc
     1501 ggcccgctct gcttggtcca ggttacggag acctggcctg gcggattggg cagcccgtca
     1561 tcggtaacgg tgccggccag gttggcaacg gccggcagag tgatcgtctg atcgggaccg
     1621 gcgtcgacct gaggagccgc atttattgcg ccgccacgcg gcggcgtcgg ggtgttgccc
     1681 aggtcttgcg tgtagattcg tagcagcgag taacacgtgc cgggagctcc gccgctttcc
     1741 tgcacgccgc gcggccctgc gcggaaggtc gctcccaact gaatgtgcaa cttctgaggt
     1801 gaccaggttc ccacggcttg cggcgacggc gggctgatct cgctcagcgt ctggcccggt
     1861 gtgcccctcc accaacggac ttgcgaaccg ttccaggtca tgcggaagcg ataggttgtg
     1921 ccgccgttcc agcccagcac gttggagcgt tcctcccgtg tggcgttgcc gccaacgacg
     1981 gtcattttca ttcggtcggt gtttccgtat gtatcgtcgt aactcttgcg gatcagggct
     2041 tgataggggc tgttgtagta gtcgccgccg gtggttgccg gattgagccc cgtgctgtcg
     2101 caagccgcca tcagctcgtt tttcgtgcag ccgctggaag gattgaggcc gctcacgtcg
     2161 aactcgattg aaccgacgtt ccacgtcccg attcggtcgt accagacgaa atcgacgctg
     2221 cgcggctcgc tcgtgttaaa ctcgtcatac ccgccggagt gcgaaagcgt gtagccgccg
     2281 gaggagaacg ttcctgtacc gtactggccg ctgatgttgc tggtgtcaac caacatgtcc
     2341 tcgaaaaccg gcaccgtggg cgcctggacc gtaacctgcc agctttcgtc gtcatgtcct
     2401 tggctgttgg tggctcgaac ggttatggtg agcgactggc cggcctgtgc cgaggttggc
     2461 gtccaaccgt acacgtagcc ggtactgccg acctgcacgc cgctcggccc gctgatcacc
     2521 gaccaagtcg gggccgggta gccgccgcac aaattgagct gtctggtgta ttcctggccg
     2581 actatcacgg tgtccgggtc cggggagact tcgcagatca ccggagggtt gggcatttgt
     2641 tgggcacgca cttcaacata atccagcttg aggtagctgt tgtcgtgcca ataggtacgc
     2701 cattcaagcc ggttgccggg agtgccgtag gcgaagtcca aagtgaagtc ctgaaagacg
     2761 tttgcctggg tgaactcctg ccgggtgatg gttcgggtgg ccaggatcgt gttcgtgtcg
     2821 attgtgtaaa cgtcgattgt caccaccggc tcgttgtcgg ccgtcacgtt gtcgatggac
     2881 atccgccaag tggcctgata actgtcggcg ggcaggcggg tgatgtacgg tccgtaggtg
     2941 aggtaacccg ctgagtcctg ggccacgttg gccgaccggg tcgagccgtc ccgccggccg
     3001 atcagcttgg ccagttgctc gtcgtattcc ggctgatagc gcaggacctt caaatcgttg
     3061 cccgcgacgt gggggctgag ccaattggtg acgttcactt ccacgctgtc ccactgggca
     3121 tagagacggc cgtcgccgtc ggtcatctgg aatgagcggt ggggcggggt gctcccggca
     3181 tttgagccgt tgtgcagaca ccaaccggcg gcaccggcca gtttgccgcc gttgcagtcc
     3241 cgcaagtaat cgtcttccac cgggttgtac gtcgtgtagc ctcgccggaa cggttcctgc
     3301 aaatggatcg ggatccgcca accgaggttg gtcatccagg tgcagtagtt gttgaccgtg
     3361 ccaatggtct gggcggaaca cccggagctc cggcacagat gcgggcagat gaaatccatc
     3421 ttggcgatgt tcttgaaatc cgacaattgg ctttgcgagt tcgggttgct cgatgcggta
     3481 cagagccggg tggggtcgat ggccttgatg gcattgatca aagcgccgca ttcagacaag
     3541 ctgacgtagc gaccgtcctg tacgtcccgc tcgttggaaa cgtcgatgta cacattgcga
     3601 taagaaagca actggttagc cagggtttgc acacaggtca ggtgctgagc ctgggtccga
     3661 gcgcccaccc acgtgccgta ggcgtcgcgg ttcaaagtga cgtccacgat gatcccgcgg
     3721 ttgttgcact cggtgatcaa cgttttgagc cgggtcatgt acggttcacg gacttgtccg
     3781 tcgtaggtca gcaccgacac atcctcaccg gcgggcgtct tccagtgccc ccagacgcgg
     3841 atccagttga agccgtcggc caccatgtca tccaggtctg aggtccggta acttgccgtg
     3901 gtgatcgatt gtgctccgta atagcttacg cccttcagat aggtgggcac cccgtcgagc
     3961 gtgaaaaacc gcccatcggc gctgatgccg atggtaaccg catacgcggg gaaacccggc
     4021 gcgcccaaca aaccgatcaa tgtgcagagg ctgatagtgt ttgtgcgacc cattctacca
     4081 catccttatg tcataaaccg atatcccgcc gcatgcccct ttgtccgggg ccctccaacc
     4141 ctgtctgttt cgtccatcgc gccgaccggg tttgcgttcc ttctcgcacc ctcgtttaac
     4201 aacccggcgg ctgatccggt cctcccaggc aatcgcggaa tttcgcgaaa tcgacttgat
     4261 ccacgtctcc atctccgtcg aagtcggctt tggcgcagtc cggtcctgac ggttgtcctg
     4321 tccctgacag gcatgcctga aagtaaccga aatcctcctg gtccacgtcg ccgtcctcgt
     4381 cagaatcgcc tgggatgacg gccgtggtga atctccacac tcgcccgtgc agaacagggc
     4441 cgctctcttg gagttcgtcc acacgccagt agtgtgcgat accctggcgc agcaaccccg
     4501 gtggatcgaa gctttcgttc ccgatgggaa acagccccat gaattccggt gagtcaactt
     4561 ccgcattgcc gaccgattcg gagtccaggc cgaagtacag gcgatggcct accgctgtct
     4621 gtcctgccgt ccaacgaagg atgattgagg ggggcacgtc aatggctccg tcggaaggta
     4681 tcggttgcgt tgccagtgca agcacctcga tcgggacatc caggctctgg ccgaaccacg
     4741 ccacgctctc ctggagcatc tggaaatgga gtgtgtaagc cccgagagcg ggtgctgtca
     4801 ggttgacggc gaaggtcttc ggccgtccgc ggaaaatgcc gccatacgtc gggatctcat
     4861 catctgagtc gtcgatcaga aaccgcgacg gtccgaaagg cgacccgcca tcgacgtcaa
     4921 caaggacaaa gtcgtccacg taaacgtcca cgaattcatc ggcggcggtt ttgcggttgt
     4981 tccagccgcc gatctcgacg gtgtggtcgg ccgggcccga cagccgaaag actcgcgagt
     5041 actcccgcca tccggtgtcc tggtcggttc cggcaccgcc tgagaatcga gccaggtagc
     5101 tttgaccgcc gagtccatag gcctgtccgt cgagctcgaa gcgggcttct ccggcctcgg
     5161 tgctctcgag ggaagcgggc agaaacagcc gataccgaaa gccaatgttc acgttcatcg
     5221 tccggtcgag atggaacgtg cgggcccagg ccgttgaggc cccgttgtag taggcggtat
     5281 tgtcgacgtt gccggtacgg gtccgcaggc cgccgccgga gaatccgtag ctcgcattcc
     5341 agtccccatt ctcgtaggcg gtgttgccgg atgtgccgaa agcgttgcgc accagggtcc
     5401 agccgtttgc agaactgtcg aacgtgcagt tcagcaaggt cggtccctga agtccgagct
     5461 tgtagaagcc ggcattcgac cactgggcgt tgccctcgtt tcgtacaaca atccttgcgt
     5521 cgatggattc acctggatac atgacggctg ggatgtcgtg ggcaagaaaa cgcgcatctc
     5581 tttccgccag tcgattgaac tgcgctgcga acgttgcggt tgtatcgatg tattcccgag
     5641 agttggccgt cgaagaccac gtgtcggtgt tcgggtttgg agggacgatg tagggcggac
     5701 cggggtcggc cgcatagatt cccgaaccct cgtcgtactc gctccaggat tcgatgtaga
     5761 cccggccata ctgggggctg tccttgatga tgctttgcca ggcgtcccga tagtgcactc
     5821 cgccgtcgcg agcaaggaag aagcccggat tacggatgtt ctggtcccag tagccgggct
     5881 tgaccgtgta gctacggacc tgtggtgagc ccgcccactc aacgaggtag gagttggttg
     5941 aaaactgcca gaccaactcg tcgcaccacg acggatagag ccccgaccag ggtgtggcga
     6001 tctggtatac gccgttgttg aaaacaggat gcgtggctcc gaaggccgcc ttcagccgcg
     6061 tttccacatc ggtgcgggtc agttggctgt agttgacgac gttctccatg tgccaggtat
     6121 cgagcatgac gcggccgttc atgattccga gatatccgtc cgcttgagca tcggggttct
     6181 tggagaagta ctggttgaag tagcgcacat attggttgac ccattcgtct ttgccggcgg
     6241 tcgtggcgac gtcagggaac ggcttgccgt accatgtgat cgcggggtcg aggaagggca
     6301 ctgctttggg cacctcgtaa ccctggcggc gcaactggca cattgcatca aacaggttga
     6361 cccgctgttg ttccgagctg gggataaggt ggacgtacat cacgtcgatg ttcgcatcca
     6421 tgatgtcctt gatctggcgg atccagaaat cgggctctcc ggtccaattc gcccgtccct
     6481 cgaccggtcg ccagggaccc gataactggc cgcccgtggc cgtataccag tggaagacgc
     6541 ttgcgctgac gagccgatcg ccggagatga actgccgcgc gggcagattg aatgtccagt
     6601 cggaggccaa caccgacgta gatgccagca gcacgaccag tccgcccggg aacccccgca
     6661 tagcttcttc tcctcacgaa ccctcttcac gcattgtagc atccggccgc cggcggaatc
     6721 caagagcaaa cgggaattcg cacaagctcg cgcccggaag caggcaattc cagggacgcc
     6781 gaggcggagc gcctttctgc gggccgaggc ccaagcatgc ggtgaccggg caccgtgcgc
     6841 gcaagccgca cagatctccg gccgtaaggt ccaaggcgga ccgccttttt gcgggccgca
     6901 acccaagcgt actgcgatcc agaaacatgc gcgcaaaccg cacagatatc gcaccgcaag
     6961 gctctagatg tcggaccaat cgcctcattc actgtcacct tcaatccgat gcaggcggtc
     7021 gggccagtcg cggccccagt gttcgagcag gtcggccagg cctccttcga accagtagat
     7081 cttcccacga atagcccgct tggcatgcgg aggcatcccg ccgatgttca cgatgctgtg
     7141 caggcagtcg gcgggatgat ggttcgtcac gtgcgaggtc cggtcccagt agatggcgca
     7201 gacccagttg ccttcgcggt tcatccgcgt gatcagtccg taatcgatgg gtgcctgtgc
     7261 cctggctttg tagggcccga tcaggggcat ggcctccagc ttgccgccgg tcagcaggcg
     7321 gtccacatgg acgcccagcg gcgtgcgggc cccgactgct tccggcgagg cgggcaaagt
     7381 cgcccaaaca ccctggcgca ctacgtacgt cagactggtc ttgtcgggct tatccttttc
     7441 atccaggttc cagaggtcgt attcactgaa agcgggggca tcggcgatcg gacggcgcca
     7501 gagctcgttg gtcttgccgc tgaggcggaa acactgctga atgccgtagt attcgttcag
     7561 gccctggtct ttggtttcca caaccatcag caggtcgacg ccatcgccgg tgggagccat
     7621 gtagaaggtc cactgttgcg ggatccagtc cttcccgtcg acctgcaccg cgtatttgag
     7681 caccttgggt cgccgggcaa agtcctgctc aaggcggatc acctcaccag gatcgatacc
     7741 ggcggcctgc agatcttgcg gaatcgggcc gtacttaagc ggcgtgtgct cgtggaaccg
     7801 gtagccgaag cgattgccgg gatgaagacg attcacgcac gtggcaaagc cctcgttctc
     7861 agtcagatac agagtggtcg gggcaatggg catcgcttgt tgcccggtgg aggagacttc
     7921 ctcggcgggc aggatgatct ggttgttcag atcgtagagt tcctcgatac catcctcggg
     7981 gtccacggcc accctgaacc gggcaccgcg cttcggggcg gcgtttctga agacggcgcg
     8041 cgctatggcc ggctccagat tcgtaacggc cggtatcccg gccatcttga cttcggagac
     8101 gaccgcccac ccgtcgccgc tttcagtttc aaggatcagg ttgaactcgc cggcctgcgc
     8161 cgacccgatg ttgtgtacgg tgacttgtac atcgtttccg gtgatcgtca catcgttgcg
     8221 gcccacagcg agatcgggaa gaagcggcgg ccgtgcgatc gccttctgta atctcaactc
     8281 gatgactgag gttctgcccg gaggaaggtc cagatggatc ggcgtccccc gggatttgtg
     8341 gatgattctg acggggggtt cccaggtgat tggtcgggcg ccgtcgctct gctggatacc
     8401 ccggttcagc agatactcac cg
//
LOCUS       DAOTPW010000252         8392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7621_length_8392_cov_4.765743, whole genome shotgun sequence.
ACCESSION   DAOTPW010000252 DAOTPW010000000
VERSION     DAOTPW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_8392_cov_4.765743"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..4179
                     /locus_tag="P5316_18175"
     CDS             136..4179
                     /locus_tag="P5316_18175"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR86941.1"
                     /translation="MERALRSFCVFWMLFNGIEAFGQPATQPVPATRPAAAILHQPDM
                     PVQGTASSPETIAAILRRAGLQTRLISAKEMADQSILNPTAFDILVLPTGESFPVEAR
                     DNVIKFLHDDGNLITMGGYAFNHLLRFENGQWRSEEDVCRTRLEQTTRKDRSLIANGD
                     MERVQELAEPGRASAGQWTRTSPNCLIVRESPYEGQWCAKAVVRPGDADKGPTFWQEV
                     PCKPGTMYQVSGWLRSERVIETGMAYIALYQHDGQGTLVTFNDFAVARGTTNWTHHKL
                     NVTTGPNVGRLVVKFGLYQTHGAAWFDDIRLSDITGGRVRPMNTSTGKPMDGLVVAPE
                     QIGMFDPSYPLKRAVGIRTAAGQRFFASEIGVSQPLTGWAASGVIGSNNARWIPLFET
                     VDRYGRHRGAAGAMLVHYNGHYAGSCWAYFGVDNVNLFAETHGAMAQGLANVARFMTA
                     KTFLRNLKTDHRFYKPGEQATASVAIDNRGRSASRVKITITASGAEGDPHEPDGAPPL
                     RQEYVVTIPAGKAEEIAAQITIPETPQNLYEVRAILEPPRGTPDSAIIDEMTTGFVVD
                     SAAAIKAGPELRFRDNYFTLNGRPMFLFGTDAYSYAYKSSYSNPLTWATDMQACVDIG
                     LNLYENLNHVRPNWEMTEDDWRDFRAMGQLSQRQGLVYMPGMLIGHNVAVGDEMLARQ
                     IALCAQYAERFRDLPGLLYYINGDYQMMLGQHPKEVEILWNRWLKDRYETTDKLRAAW
                     APTPVNAELGSLAFWPPDGGKWNDTAIVDRMRFQNWLTRRWNESHVAAIRKHDNGHPI
                     TSEYYQIPFAGMDLIQTIDGQDVSNFGFFSTPGKDIDILPLKIRFNDLRVRGKGVALG
                     EYGVKTHPAWTVENGATDYHIVRSEEEQIRLFMAVAHYALGMGAGKVQNWCLQDAQDS
                     VFPWGLFYPNRLIPKDVAYVHRNQSLIWRHFAPRYVPSRLTVCIPNNLRLGNFEAIGR
                     DVADRTFETLLGLHYDFNVIDDHHLADVPDDTKAIIYPSPFSLGDETYQQLLTLARRG
                     VHILVTGDISYDENRKRTRTQRLKELAGVEFVGEEYPNIERPMEASMDTTSTKPNDLA
                     DALYPCIRVRPSAAQVLQARADGTPLLVRHNDVFLCLDPLELGGDAAAAEPRRHVYSL
                     FLETTSAKPLPIEPNEPWLHVMAQPTAKGTVHVVYNRRTDPGHQEVTVHTAAGHVRLN
                     TKNGWPALAAVTDEGKVVAVNAFGEASIGDEPLLRGMGMKALLSLDGQDLRQSQAILV
                     TPFQPGTLMMSSSTTADDTASVHVLDFSGGQWREHENLSIDSRNAMVEIDSDRATCVL
                     VLDRGSDGRWREHLTQAMLRPASIDGY"
     gene            4215..6854
                     /locus_tag="P5316_18180"
     CDS             4215..6854
                     /locus_tag="P5316_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86942.1"
                     /translation="MRIHRMHRSTVAVLAMLANLICPQPSLAAEPFDYFSNSWSVIGL
                     KDYKDGTRITPDNRLLLSDGCELRIRIGQGKHRLSREQTKTLMDGWLPVVQLTAQDES
                     IRYDMTMWATPLPTVKDWQKAFDWPTDGDNYLNWITVKAANTGQSTATADLDAVIVQP
                     HSGQITSMGKLSRDLKAGETAEMVLRVPFESLDTTIAVDFEKEDPAVWLERTVKYWRD
                     LVLSAGRITVPCEKSTQALLAAHVCQLIANDHGQLRGGEGFYDEFYIRDGAYQVMELE
                     EAGLDDPAAKAIRHYLATQRPDGRFETQKGQFDANGQAVWALWQYYKITGDPAWLAEV
                     YPQMKRAVDWTMQARRQATADSPFAGVLPAAVADGEYLWDGKHHIVGYDFWNLRGMIC
                     TADAAGILGKTGDQDALLTEIKDYRSAIDAAWKRTGLDHFPPSWEKAGTHWGNTETLW
                     PTALFDVNDPRMAAMQKEVRQRHGGGFIEGTIRWTGLPDAIHPYMSAYTTMAALVRGE
                     HDQVVEDFYWYLLHSSATHAFPEGIFYKRRFAWSDTIPHTTGAANYAIMLRHMLIHEQ
                     EDELHLLAGVPDGWLAEGKEISVDRAPTHFGPVDMLVSGTAKGVFVQFKGADRQKPRR
                     VVLHLPASRPLEASIPGVSVETRPAQQKQWDYAGVVEMYAKTAPPLPRTIRDVVDLPA
                     DPAIPADRCVLLDLSKLANTDPFTAPFGVANPGKFLLTGLPVGDQTVCGIPFRIIDPT
                     ANNGKGLIVLASDKAPADREWPTQVEITVSGVAKRAFFLGNVTGWGATDAGVGEWGAV
                     GAYEVEYADGQKQVVPLITGYTCEDWTSAPRATGVTCGLRGKPWHLNVMGVKLRQAEL
                     KRIIFCDYGTPAAPVLAAVTLER"
     gene            7275..8381
                     /locus_tag="P5316_18185"
     CDS             7275..8381
                     /locus_tag="P5316_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86943.1"
                     /translation="MRRSSVLAVLAISSAAMGENLLLNPSFTDPPVAPPCAEDWQDIS
                     NWEMTFQSSPAGSGERRNGDYKSPPCPRDGDGTHASLQGWGRENKLAWQTVTGLTTGN
                     YYKLSGLWFYGADSYGGGRLTVTAEIRTGTDPSGGTLIASVQSTIANGITSMWLPFQT
                     CGQLPFGNEVTVVTRASYNDWVGWALHFDNLVLEEVPNCVEPNRVDEIVPAYGTRGTT
                     IDATITGFDFTRGPVSVRLNRPGASDIHATNLSVQSSNVLTCRFALTGAANGRWNVMV
                     SLGSGEPLTATLPNGFMVVLPTLSNGSFELPTAEGGCPVTPLWGHPTDWLVLHSGSWG
                     DAGYDATTVVRDRVDVVPPACCRRRSEHAVNPPV"
BASE COUNT         1695 a         2593 c         2559 g         1545 t
ORIGIN      
        1 caccgcatca tcaaaccggc ggatttacag catgttcaga ccggcgccga cacggcccgc
       61 atggtaccaa acgttcgttg cgattaccat cgtcggcgtg acgccgatat tgcgaacctg
      121 caaggagtat gacgtatgga gcgtgctttg cggtctttct gtgttttttg gatgctgttc
      181 aatggaattg aggcgttcgg gcagccggcg acccagccgg tacccgccac gcggcctgct
      241 gccgcgatcc tgcaccagcc ggacatgccg gttcaaggaa cagcctcgtc gcccgagacg
      301 atcgcggcca tcctgcggcg ggccggtctc cagacgcgac tgatctccgc caaggagatg
      361 gcagaccaga gcattctcaa tccaacagct ttcgacatcc tcgtcctgcc gaccggcgag
      421 agcttccctg ttgaggcccg agacaatgtc atcaagtttc tccacgacga tggcaatctg
      481 atcaccatgg gaggatacgc cttcaatcat ctgcttcgat tcgagaacgg acagtggcga
      541 agcgaagagg atgtctgcag aactcgcctg gagcagacca cgcgcaagga tcggtccctc
      601 atcgctaacg gcgacatgga acgcgtgcaa gaactggcgg agcccggccg tgcgtccgcc
      661 gggcagtgga ctcgcacctc gcccaactgc ctgatcgtac gcgagtcacc atatgaaggt
      721 cagtggtgtg ccaaggcggt cgtgcggccg ggggacgccg acaagggacc gacgttctgg
      781 caggaagtgc catgcaaacc cggcaccatg taccaggtct ccggctggct tcgctcggaa
      841 agggtcatcg agacgggcat ggcttacatc gccctgtatc agcacgatgg ccagggcacg
      901 ttggtgacct tcaacgactt tgccgtggct cgcggtacca ccaactggac acatcacaag
      961 ctgaatgtca ccaccgggcc caatgtgggg cgactggtgg tcaagttcgg cctttaccag
     1021 acacacggcg ccgcctggtt cgatgacatc cgcctgtcag acatcaccgg aggccgagtt
     1081 cgtccgatga acacctccac cggcaagccg atggacgggt tggtcgtggc acctgaacag
     1141 atcggtatgt tcgatccctc gtatccgctc aaacgcgccg tcggaatccg cacagccgct
     1201 gggcagcgat tctttgccag tgagatcggt gtgtctcagc cgttgaccgg ctgggcggcc
     1261 tcgggagtga tcggcagcaa caacgcccgc tggattccgc ttttcgagac cgttgaccgc
     1321 tatggccgtc atcgcggagc cgctggagca atgctggtcc actacaacgg ccactatgcg
     1381 ggttcatgct gggcctattt cggcgtcgac aatgtcaatc tgttcgccga aacgcacggg
     1441 gcgatggccc aaggtctggc aaatgtggcg cgattcatga cagccaagac cttcctgcga
     1501 aacctcaaga ccgaccatcg attctacaag cccggcgaac aggcgacggc ttcggtcgcc
     1561 atcgacaacc gtggccggtc ggcaagccgg gtcaagatca ccatcaccgc ttccggcgcg
     1621 gaaggcgatc ctcacgaacc ggacggcgcg ccgccccttc gccaggaata cgtcgtgacg
     1681 atccccgcgg gcaaggccga ggaaatcgcc gcccaaatca cgatccccga gaccccgcag
     1741 aacctgtatg aggtcagagc gatcctcgaa ccgcccaggg gcaccccgga cagtgccatc
     1801 atcgacgaaa tgaccacagg tttcgtggtc gacagtgctg ccgccatcaa ggccggcccc
     1861 gaactgcgtt tcagggacaa ctacttcacg ctgaacggcc ggccgatgtt cctcttcggc
     1921 acggacgctt attcttacgc ctacaagtcg tcatacagca atccgctgac ttgggcaacg
     1981 gatatgcagg cctgcgtgga catcggcctg aatctctacg aaaacctgaa ccacgtccgt
     2041 ccgaactggg agatgaccga ggatgattgg cgagactttc gcgccatggg gcagttgtcg
     2101 cagcgtcaag gcctggtgta catgcccggc atgctcatcg ggcacaatgt tgcggtcggc
     2161 gatgagatgc tggccaggca gatcgctttg tgcgcccagt atgccgagcg attcagggat
     2221 ctgccggggc ttctgtacta catcaacggc gattatcaga tgatgctcgg ccaacatccg
     2281 aaggaggtcg agatcctctg gaaccgctgg ctcaaggatc gttacgagac aacggacaag
     2341 ctgcgcgcgg catgggcgcc gacgccggtc aacgcagagc tgggcagtct ggctttctgg
     2401 ccaccggacg gcggaaaatg gaacgacacg gccatcgtcg accgcatgag gtttcagaac
     2461 tggctcaccc ggcgttggaa cgagtcgcac gtcgcggcga tccgcaaaca cgacaacggc
     2521 cacccgatca cttcggagta ctatcagatc ccctttgccg ggatggacct cattcagacc
     2581 atcgacgggc aggatgtcag caacttcggc ttcttcagca cgccgggcaa ggacatcgac
     2641 attctgccgc tcaagatccg gttcaacgac ctgcgggttc gcggtaaggg agtcgccctg
     2701 ggcgagtacg gcgtcaagac gcacccggcc tggaccgttg agaatggggc aaccgactac
     2761 cacatcgttc gcagcgaaga agagcagatt cgactgttca tggccgtcgc acactacgca
     2821 ctgggcatgg gagccggcaa ggttcagaac tggtgcctgc aggatgcgca ggattccgtt
     2881 ttcccttggg ggttgttcta tccgaatcgg cttatcccga aggacgttgc gtacgtgcat
     2941 cgcaatcagt cgctcatctg gcggcatttc gcgccgcgtt acgttccatc caggctgacg
     3001 gtgtgcatcc cgaacaacct gcggctgggc aacttcgagg ccatcggccg ggatgtggcc
     3061 gaccgcacct tcgaaaccct gctcggcctg cattacgatt tcaacgtcat cgacgaccat
     3121 caccttgcgg acgtgccgga cgatacgaag gccatcatat acccgtcgcc cttttctctt
     3181 ggtgacgaga cgtaccagca gcttctgacg cttgcgagaa gaggcgtgca tatcctggtc
     3241 accggcgaca tctcatacga cgagaaccgc aagcggactc ggacgcagcg tctgaaggag
     3301 ctggccggcg ttgagttcgt tggtgaggag tatccgaaca tcgaacggcc gatggaagcg
     3361 agcatggata ccacgtccac gaagccgaac gacttggccg acgccctgta cccgtgcatt
     3421 cgggtgcggc cgtctgcagc acaggtcctc caggctcgcg cggacggcac ccccctgctg
     3481 gttcgccaca acgacgtctt cttgtgcctc gatccgctcg aactgggcgg tgacgccgct
     3541 gccgccgagc cccgtcgcca cgtctactcc ctgtttctgg agacgacgag cgcaaaacct
     3601 ttgcccatcg agccgaacga accgtggttg cacgtcatgg cccaacccac tgccaaaggc
     3661 acggtccacg tggtttacaa tcgtcgaaca gacccgggac atcaggaagt gaccgtccac
     3721 acggccgcgg gccacgtgag actgaacacg aagaatggat ggccggcact ggcggccgtc
     3781 acagatgagg gtaaggtcgt ggcggttaac gcattcggcg aggcttcgat cggcgatgag
     3841 ccgttgctgc gcggtatggg catgaaggcc ttgttgtcgc tcgacggcca ggacctgcgg
     3901 cagtctcagg cgatcctggt tacgccgttc cagccaggca ctctcatgat gtcgtcttca
     3961 accacggcgg atgacacggc gagcgttcat gtgctggatt tctcgggcgg tcaatggagg
     4021 gaacacgaga acctgtcgat cgactctcgc aatgcgatgg ttgagatcga ctcggacagg
     4081 gcgacatgcg tgctggtgct ggatcgcggg tcggacggcc gatggcggga gcacctgacg
     4141 caggctatgc tgagacctgc atcaatcgac ggttattgac ggccggagca ccatgtccgt
     4201 gcaggaggca aatcatgagg attcaccgca tgcaccgttc cactgtagcc gtgctggcca
     4261 tgctggccaa tctgatctgc ccgcagccat ctcttgcggc cgagcctttc gactacttct
     4321 ccaactcatg gagcgtcatc ggtctgaaag actacaaaga cggcacgcgg atcactcctg
     4381 acaaccggct tctgctgtcc gacggttgcg agttgcggat ccggatcggt caaggcaagc
     4441 atcggctcag ccgggaacag accaagacgc tcatggatgg gtggctgccg gtcgtgcagc
     4501 tcaccgccca agatgaaagc atccgctatg acatgacgat gtgggccacc cccctgccga
     4561 cggtcaagga ctggcagaag gcgttcgact ggccaaccga cggcgacaac taccttaact
     4621 ggatcacagt caaagccgcc aacacgggtc aatcgacggc aaccgcggat ctcgatgcgg
     4681 tgatcgtgca accccacagc ggccagatca cgtccatggg caagctgtcg cgagatctca
     4741 aagccggcga gacggccgag atggtgctgc gggtgccctt cgagtcgctc gatacaacca
     4801 ttgcggttga ctttgagaag gaagaccccg ccgtgtggct cgagagaaca gtcaagtact
     4861 ggcgagactt ggtcctcagc gccggccgaa tcacggtccc gtgcgaaaag agcacgcagg
     4921 ctctgctggc cgctcacgtg tgtcagctca tcgccaacga ccacggccag cttcgcggcg
     4981 gcgaggggtt ttacgacgag ttctacatcc gcgacggggc ctaccaggtg atggaactgg
     5041 aggaggccgg tctggacgat ccggcggcca aggccatccg gcactatctt gccacccagc
     5101 ggccggacgg acgtttcgag acccagaagg gccaattcga tgccaacggg caggcagtgt
     5161 gggccctgtg gcagtactac aagatcaccg gcgatccggc atggttggcg gaggtctacc
     5221 cgcagatgaa gcgggcggtc gactggacca tgcaggcccg gcgtcaggca acggccgact
     5281 cgcctttcgc gggcgtcttg cctgcggccg tggcggacgg cgagtatctc tgggacggca
     5341 aacaccacat cgtcggctac gacttctgga acctgcgagg catgatctgc acggccgacg
     5401 ccgccgggat cctcggcaag accggcgacc aggacgccct gcttacggag atcaaggatt
     5461 atcgctcggc catcgatgcg gcatggaaaa gaacaggact cgatcacttt ccgccgagtt
     5521 gggagaaggc cggaacccac tggggcaaca ccgagacgct ctggccgacg gcccttttcg
     5581 atgtcaacga tccccgcatg gccgccatgc agaaggaggt tcgccagcgc catggcggcg
     5641 ggtttatcga aggcacgatt cgctggaccg ggctgcctga tgcaatccac ccctacatgt
     5701 cagcctacac gacgatggcg gccctggttc gcggcgaaca cgatcaagtc gttgaggatt
     5761 tctactggta cctgctgcac tcgtcggcga cgcacgcctt tcccgagggc attttctaca
     5821 aacggcgatt cgcctggagc gacaccatcc cccacaccac gggcgcggcc aactatgcga
     5881 tcatgctccg ccacatgctg atccacgaac aggaagatga gcttcatctg ctggcgggcg
     5941 tgcccgacgg ctggctggcg gagggcaagg agatcagcgt cgatcgcgcc cccactcact
     6001 ttgggccggt cgacatgctt gtgagcggca cagccaaagg cgttttcgtg cagttcaagg
     6061 gcgcagatcg acaaaaaccc agacgagttg tgctccatct gccggcctcg cgtccactag
     6121 aagcttcgat ccccggcgtg agcgtcgaga ctcgtccggc tcagcagaaa caatgggact
     6181 atgccggcgt ggtagagatg tacgccaaga ccgccccgcc gcttcctcga acaatccgcg
     6241 atgtggtcga tttgcctgcc gacccggcga taccggctga tcgctgcgtg ctgctcgacc
     6301 tgtcgaaatt ggccaacacc gatcctttca cggcgccgtt tggcgtcgcc aatccaggca
     6361 agttcctcct caccggcctg ccagtgggcg accagactgt ctgcggaatt cctttccgga
     6421 tcatcgaccc gaccgcaaac aacggtaagg gccttatcgt tttggcttca gacaaggcac
     6481 ccgccgaccg cgagtggccc acacaggttg agatcacggt cagcggtgtg gccaaacgag
     6541 cgttctttct cggcaacgtg accggttggg gggccaccga cgccggcgtt ggcgaatggg
     6601 gggccgtggg cgcgtacgag gtcgaatatg ccgacggaca gaagcaggtt gttccgttga
     6661 tcacgggcta cacctgcgag gattggacct cggcaccccg cgcaaccggc gtgacctgcg
     6721 gtctgcgcgg caaaccgtgg cacctcaacg ttatgggcgt gaagctgcga caggccgaac
     6781 tcaagaggat catcttttgc gactacggca cccctgctgc gccggtgctg gctgcggtga
     6841 ccctcgagcg atgaggtaca ggcaacatgg ccacgcagag ccgccgtcat ggcacccaac
     6901 aacctggcgg acgccgacgc ttggcccctt gcaagtccgg ggcgaccaag gaaccgccgg
     6961 cgagtctgat tcccgcccct gccatgtaca agtccttgta tcagcaaagc ctcggatgaa
     7021 agctgcggga atcgccggta ttcgccgagt tgtgtattga cacgagggca gccaccgggg
     7081 tacattggat ggtaggaatt gtcttatcta aaacttttta gctcatcgcg gtaggcggct
     7141 gcgtccgatg tactggatcg ataagaggca acgttgctca acctgtcgac gtgccaggtc
     7201 actcgacccg atgcaacgga atctttgatc ttcctcaagc atcgcgacga ctgcctatca
     7261 ggaaggaggc cccgatgcgt cgcagcagcg tcctagccgt tcttgcgatc tcttcagcgg
     7321 caatgggaga gaaccttctg ctcaacccga gtttcaccga cccgccggtt gcgccacctt
     7381 gcgctgaaga ctggcaagac ataagcaact gggaaatgac gttccagagt tcaccggccg
     7441 gttcgggcga gcgtcgcaat ggagactaca agtctcctcc ctgcccccga gacggcgacg
     7501 gcacacacgc cagcctccag ggttggggcc gcgagaataa actggcttgg cagacggtca
     7561 ccggcctcac caccggcaac tactacaagt tgtcgggcct ctggttctac ggcgctgact
     7621 cctacggcgg cggccgcttg accgtgaccg cggagattcg cactggcact gaccctagcg
     7681 gcggcaccct gatcgcttcc gtccaaagca ccatcgctaa tggtattacg tcgatgtggc
     7741 tgcctttcca gacctgcggc cagttgccgt tcgggaacga ggtgacggtg gtcacgaggg
     7801 ccagctataa cgattgggtg ggctgggctc tccatttcga caacctcgta ttggaagagg
     7861 tgccgaattg tgttgagccg aatcgggttg atgagattgt gccggcatac ggcactcgag
     7921 gcaccacgat cgacgcgacc attacggggt tcgacttcac tcgcggcccc gtttcggtca
     7981 ggctcaaccg ccccggggct tcggacattc acgcgacaaa cctgagcgtt cagtcatcca
     8041 acgtactgac atgcaggttt gccctgaccg gggctgccaa cgggcgctgg aacgtgatgg
     8101 tgagccttgg aagcggcgag ccgttgaccg ccactttgcc caacggcttc atggtcgttt
     8161 tgccgaccct ctcgaacggc agctttgaac tgccaacggc ggaaggtggc tgcccggtca
     8221 cgccgctttg gggccatcca acggactggc tggtattgca cagcggcagt tggggcgacg
     8281 caggttatga tgcaacgaca gtcgttcgcg accgcgttga tgtcgtgccc ccagcttgct
     8341 gtcggcgccg gtctgaacat gctgtaaatc cgccggtttg atgatgcggt ga
//
LOCUS       DAOTPW010000253         8364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7649_length_8364_cov_5.215549, whole genome shotgun sequence.
ACCESSION   DAOTPW010000253 DAOTPW010000000
VERSION     DAOTPW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7649_length_8364_cov_5.215549"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1375)
                     /gene="amrB"
                     /locus_tag="P5316_18190"
     CDS             complement(140..1375)
                     /gene="amrB"
                     /locus_tag="P5316_18190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRR86944.1"
                     /translation="MPMTTLPDKPKLRRVEAFPVSDDARGPLYVIRDPCGLAPGLITL
                     SPVAMFILSLMDGEHGLLDIQEIFARHTLQILPRQQLEDMVRQLDEAHFLDSPAFEAY
                     FNSLVEAYRAAPARISGSEESFGAPPGQMGTVIQKMLFDGQDMPARSNRRLLGLVAPH
                     LDYPRGAPAYSRAYRMLAATQPPRRVIILGTNHFGQASSAVATRKDFQTPLGTTGTDR
                     AFIEALENKLGTSLCEHEFDHQREHSVELQLLILQHLLRPEQFEIVPVLCPDVCGPTG
                     TAPYDGKGVDLRDLGEAIGELIRADRTPTLIVAGADLSHVGGQFGDERDLDAAFLQEV
                     EQKDREALDALINQGCEAFVSVLKSRENDTRVCSAGCIYAMMTALRGARPELLTYHQA
                     ASPQAGNGVTCAAIAVWEK"
     gene            2166..6245
                     /gene="secD"
                     /locus_tag="P5316_18195"
     CDS             2166..6245
                     /gene="secD"
                     /locus_tag="P5316_18195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRR86945.1"
                     /translation="MGEKNLWSKVGLIVIFAGLSLWQTYPFTTEQLKPGIDLGGGHSL
                     LFEIDDTGDSSYDLAKRVMNVLKNRVDPQGNRNLIWRPIGRNRLEIQIPRAPEHQRRN
                     REKYNHARQALIDTYISEPQIRQALRLPPAQRDEAINRLVGKYESRRPLFEELVRVDE
                     QYRELSSRFEKEGATTQPAQTQPSAETSLAQQVDDAFKKRNELIKQLVAKNLEIRVLE
                     DLLALGRKSATRVEGIERLKSEHSDLAELIDNMIRTYDACARYRGSLDDPADLMRLLK
                     GAGVLEFRILATRSPGNLAMTDAKNPEYQVEISTYENNLSRFGPRVQTGDKFMWIKIA
                     KPDPGDWSERYYIIREYAGSQYVLAHATPDMGLLRNTGWTLAGAYPGRDRLGRLAIDF
                     QLGGNGPAQFWELTNNNIGRPLCIVLDDEAISSANIHSAISDRGQITGRFTPQRVDYI
                     VRTLEAGSLPAKLKEVPLQQNSVGPSLGETNRRMGFRASVMAFVVVAVFMAVYYFYNG
                     AIADIALLLNMVITLGIMSFLQATFTLPGIAGLVLTLGMAVDANVLIYERMREELQRG
                     ISVRMAAKLGYEKAFSAILDSNVTTIITAVILGVIGSEEIRGFGLTLGIGLCTSMFTA
                     LFVTRQYFHIMVPNTLNREETNKAWLGTGILVVAAGVLMGTGYLANNPDTLQDSRLYG
                     LGVFVAWLAGTAVVLMAGLWLFRFLYGATGHRQANRLPMFKLLSAPNINWMAKYRIFW
                     ACSAAVIGIGLLGMFMVKPADYMDIEFIGGTSVQIEIKADSQKQLNDEKIQQLVANDA
                     ANWLRQAATWLAGATVTEVGENRFRVTTPGDLTASQITSLLMPTLEEDDAVARNGVTP
                     TEDGKGVIVQVKVPTRTSEEEAPKPLDLAGVQEEVRKAAEYASNAWRNLMSARVQTIE
                     LASEGEKREAYEVIVTEPRKTLVAEAMLATMGEILEVTPAVDARLETDPDRAPEGIYP
                     IKSDAVVLADVIGGHSQAQIVQRVRRGEAMESDTIDFHGGLVMVFDGLNPAQSAAQLE
                     ARIKRMRLQPGFEDQGSRPTRVIGLEAAGGGDPAVDPCTRVAIMVKDPVFSYLDEEDN
                     TKWLGDVARKEVNLVKEALATSLSLQRVTQFSPQVASEAITKAIIAVVLSLIAIAAYL
                     WIRFGSLTFGLAGILALYHDVAIALAGIFIAHHVWHTRVGEVLMLRDFRIDLNIVAAL
                     LTIVGFSINDTIVIFDRIRENRGRIATISARLINDSLNQTLSRTILTTLTVLMTLFVM
                     YFFGGEGIHGFAFAMIVGSLSGTYSTLAIATPLIHHPRILWVVTVVLAGLTLAGLAWM
                     IPGRMVSRVLPLVILGLSVVVLVVLARSFAREDRAASPA"
     gene            complement(6359..7687)
                     /locus_tag="P5316_18200"
     CDS             complement(6359..7687)
                     /locus_tag="P5316_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR86946.1"
                     /translation="MPLSNAAVAEPPAQTASSATTNIYQPVPPESLEQAGLTNEMVDA
                     LIFKYLMNVGSAPGAKIASTLALPHPLVVERLTDLKKQQLLGYVGNANMGDFTYTLTE
                     AGRGRARQFMEESMYFGAAPVPLDAYCKAVAAQSITNEKPGPDQLRNAFSDLLINERM
                     FSTLGPAINSGRGLFLYGPPGNGKTSIAERITRCFGSEIYIPYAIHIDGFIVKLFDPE
                     IHERIPLPKSSIFKTAAVDERWVRIKRPTVVVGGELTMDQLEIRWNPTTRVSEASMQL
                     KSNCGTLVIDDFGRQRMNPFELLNRWIVPLEKRFDFLALANGVKIQVPFDQLLIFSTN
                     LEPKDLVDDAFLRRIPYKIHVDNPSEEEYRKLMQMMCDFLGIKYDDALVTKLIDKHYA
                     QTGRPFRCCQPRDLVMLSKNYCEYNKIPVHLDEETLDYAVSVYFTVMEGT"
     gene            7980..>8364
                     /locus_tag="P5316_18205"
     CDS             7980..>8364
                     /locus_tag="P5316_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86947.1"
                     /translation="MVIRLRSVLVAMVVAWPALLVPPVLRAGDMPVGGVHVKILPPLP
                     VWPSDYETITERRPTFHICGLSSATKYRVELARDAAFADGFTLTEFEITDTSGISPVV
                     LVAYQGEPLPDGQYYWRAFCGDDEGF"
BASE COUNT         1538 a         2362 c         2783 g         1681 t
ORIGIN      
        1 tgtccaccgc gacgccgggg aatgaagaac ggcgaacgcg taattgagga tggggcttgt
       61 tccgtcgcga tcggcgaaat gcgccggcac acggcttggt ccgcgagcgc gacccttgga
      121 tgcttgcaca actgaggggt tatttctccc acaccgcgat agcggcgcag gttacgccgt
      181 tgcctgcctg gggactggcg gcctgatgat aggtcagcag ttcaggtcgg gcgccgcgca
      241 gggcggtcat catggcgtag atgcagcccg cgctgcacac gcgggtgtcg ttctcacggg
      301 acttgagcac gctgacgaag gcctcgcatc cctggttgat caaggcgtcg agtgcctccc
      361 ggtctttttg ctcgacctct tggaggaagg cggcgtcgag atcccgctcg tctccgaact
      421 gcccgccaac gtggctgagg tccgccccgg ccacgatcag ggtaggcgtg cgatccgccc
      481 ggatcaactc gccgatagcc tcacccagat cgcggaggtc tactcccttg ccgtcgtacg
      541 gggccgtgcc ggtcggtccg cagacgtccg ggcagagtac cggaacaatc tcgaactgct
      601 cggggcggag caggtgttgg aggatcagca actgcaactc gacggagtgc tctctctgat
      661 ggtcgaactc gtgctcgcag aggctggtcc cgagtttgtt ttcgagagct tcgatgaacg
      721 cgcggtctgt cccggtggtt ccgagcgggg tctgaaaatc ctttcgtgtg gccacggctg
      781 atgaggcttg gccgaaatgg ttggttccca ggatgattac ccgtcggggc ggctgtgtgg
      841 cggccagcat ccggtatgcc ctggaatagg ccggcgcccc ccgcgggtag tccaggtgag
      901 gtgcgaccag accgagcagc ctgcggttgg acctggccgg catatcctgg ccgtcgaaca
      961 gcatcttctg gatgacggtc cccatctggc ccggtggggc cccgaaggac tcctcgctgc
     1021 ccgagatgcg tgccggggcg gctcgatagg cttcgaccaa ggagttgaaa taagcctcga
     1081 aggcggggct gtcgaggaaa tgggcctcat cgagttgtcg gaccatgtct tcaagctgct
     1141 gccggggcag gatctggagg gtgtggcggg caaagatctc ctggatgtcc agcaggccgt
     1201 gttcgccgtc catgagcgac agtatgaaca tggcgaccgg ggacagggta atcaggcctg
     1261 gtgccaagcc gcaggggtcg cggatgacat agagcgggcc gcgagcgtcg tcgctcacag
     1321 gaaaggcttc gacgcgcctg agcttgggtt tatcgggcag cgttgtcatt ggcatggttc
     1381 cccctatgtg tgcggccggg tgggcgagtt gcccccaagg gccagtgctc ccaggatacc
     1441 acaaagctgc cagatgcccc agacaggggc agggaagcaa gccgcggggc ccccgcctcc
     1501 ctgtttcagt cgccggccag gttgatgccc ttggcccggc cgcggtatca tacgcaatct
     1561 tatgcaggga acggacgagt cccaatccga tgtatctgcg ctgaggccgg cttggctggt
     1621 tgctctcgtg ctggtggctg gcacgctttc aacgcgtgcg tgggccatgc cgatcgagaa
     1681 cgactccgcg aaaaccggtt cgtcccggtc tgattccacg accaggtcca gcgactcccg
     1741 accggaatcg gagaaccgcc aaaggtttga aatcgcgccg caggcggacg tctggcacgg
     1801 cgtcgcggca cgcccgccgg tggagcggcc gatcggcgct tccgggcatc tttccacggt
     1861 tgacttctgc caagccccgt gccgtcgcgg agcgacgatc ctgtggatcg agccggcacc
     1921 ggggggttcg ccgagacagc gctgtcgact tatctgtcgt gaatcccatg cacctccttt
     1981 catggggtag atgacgcttg gtgagggcaa gtcatcgccc gtggaatcat cttcccgtgt
     2041 acttgcagcc gcctcgccgt ggttcggaat gactggactt atcacaaaga aatgccgtta
     2101 gtggacaggt gaatgagacg aggacatccg gcgacggatg ccattgcgat tctgaggtaa
     2161 ttgtcatggg cgagaagaat ctgtggtcga aagtcggcct gatcgtgatt tttgccggcc
     2221 tgtcgttgtg gcagacctac ccttttacga cggaacagct caagccgggc atcgacctgg
     2281 gcggcgggca cagtctgctg tttgagatcg atgacacagg ggacagcagt tacgaccttg
     2341 ccaagcgggt gatgaacgtg ctcaagaacc gcgtggaccc gcaagggaac cggaacctca
     2401 tctggcggcc gatcgggcgc aaccgccttg aaatccaaat cccgagggcg cctgagcatc
     2461 agcggcgaaa ccgcgagaag tacaaccatg cccggcaggc cctgatcgac acgtacatca
     2521 gcgagccaca gatccgacag gccctcagat tgcctccggc ccagcgcgat gaggcgatca
     2581 atcggctggt gggcaagtat gagagccgaa gaccgctgtt cgaggagctg gtcagggtgg
     2641 acgagcagta tagggagctg agcagtcgct tcgagaagga gggtgcaacg acgcagccgg
     2701 cacagactca gccttcggct gagacgtcgc tggcgcaaca agtggacgat gcgttcaaga
     2761 agcggaacga gttgatcaag caactggttg ccaagaacct cgaaatccgg gttctggagg
     2821 acctgctggc tctgggcaga aagagcgcca ctcgcgttga gggaatcgag cggctcaaga
     2881 gcgaacacag tgacctcgct gagctgatcg acaacatgat ccgcacgtac gatgcgtgcg
     2941 cccgatatcg gggttcgctc gatgatccgg cggacctgat gcgtctcctg aagggggccg
     3001 gtgttctgga gtttcgcatc ctggcgacgc gctcaccggg caacctggcg atgaccgacg
     3061 cgaagaatcc ggagtaccag gtggagatct caacctatga gaataacttg tcgcggttcg
     3121 ggccgcgcgt ccagaccggt gacaagttca tgtggatcaa gatcgccaag ccggatccgg
     3181 gcgactggtc tgaacggtac tacatcattc gtgagtacgc gggttcgcag tatgttctgg
     3241 cccacgcgac gccggacatg ggattgttga gaaacacggg gtggacgctg gccggggcat
     3301 atcccggccg cgaccggttg ggtcgcctgg ccattgactt ccaactgggc gggaacggtc
     3361 cagcgcagtt ctgggaattg accaacaaca acatcgggcg gcccttgtgc atcgtcctgg
     3421 acgacgaggc catctcctcg gcgaacatcc acagcgccat ctcggacagg ggccagatca
     3481 ccggccggtt cacgccgcag cgagtcgact acatcgttcg cacgttggag gcgggttctc
     3541 tgccggccaa gctcaaggaa gtcccgctgc aacagaacag cgtgggcccc tcgctgggtg
     3601 aaaccaaccg ccggatgggt ttccgggcct cggtcatggc gttcgttgtg gtggcggtgt
     3661 tcatggccgt ctactacttc tataacggcg cgatcgccga tatcgccctc ttgctgaaca
     3721 tggtcatcac gctgggcatt atgagctttc tgcaggccac gtttacgctg ccgggcatcg
     3781 ccggtctggt gctgacattg ggcatggcgg tggacgcgaa cgtgctgatt tatgagcgca
     3841 tgcgcgagga gttgcagcga ggcatttcgg tcaggatggc cgcgaagctg ggttacgaga
     3901 aggcgttcag cgccattctc gactcgaacg tgaccacgat tatcacggcc gtgatcctgg
     3961 gagtcatcgg cagcgaagag atcagaggtt tcggcctgac gctgggcatc ggcttgtgca
     4021 caagcatgtt tacagccttg ttcgtgaccc gccagtattt ccacatcatg gtgcccaata
     4081 ccttgaaccg agaggaaacg aacaaggcct ggctgggcac gggcattctg gtggtagcgg
     4141 caggggtatt gatgggaaca gggtacctgg cgaacaatcc cgacactctt caggattcgc
     4201 gtctttacgg gcttggtgtc ttcgtggcct ggctggcggg aactgcggta gtgttgatgg
     4261 ccggcttgtg gttgttccgt ttcctgtatg gggcaaccgg ccaccggcaa gccaaccgtc
     4321 tgccgatgtt caaactgctg agcgcaccga acatcaactg gatggccaag tatcgcatct
     4381 tctgggcctg ctccgcggcg gtgatcggca tcggcctgct cggcatgttc atggtgaagc
     4441 ctgccgatta catggacatc gagttcatcg gaggaaccag tgtccagata gagatcaagg
     4501 ccgacagtca gaagcagctc aacgatgaga agattcagca gcttgtcgcc aacgacgcgg
     4561 ccaactggct caggcaggcc gcaacgtggc tggccggcgc gacggtcacc gaggtgggcg
     4621 aaaaccgttt ccgggtcacg acgccgggcg atctgacggc gtctcaaatc acgtcgttat
     4681 tgatgcccac ccttgaggag gatgatgcgg tcgctcgtaa cggcgtcacg ccgaccgagg
     4741 acggtaaggg cgtgatcgtc caggtcaagg ttcccacgag aacctccgaa gaggaagccc
     4801 ccaagccgtt ggacctggcc ggcgttcagg aagaggtgag gaaagcggcc gaatacgctt
     4861 caaacgcctg gcgcaatctg atgtcggccc gcgtgcagac gatcgagctg gcctcggagg
     4921 gtgagaagcg agaggcatac gaggtcatcg tcaccgagcc gcgcaagacg ctggtggccg
     4981 aggcgatgtt ggcgaccatg ggggagatcc ttgaggtgac gcccgcggtc gacgcccggc
     5041 tggaaaccga ccccgacaga gcccctgaag ggatctatcc aatcaagagc gacgccgtgg
     5101 tgttggcgga cgtcatcggc ggacactctc aggcccagat cgtccagagg gttcgacggg
     5161 gcgaagccat ggaatcggac acgatcgatt tccatggtgg actggtcatg gtctttgatg
     5221 gtttgaaccc cgcgcaaagt gccgcccagt tggaagcgag aatcaagcgg atgcggctgc
     5281 agccgggctt cgaggatcag gggtcgcgcc ccacgagagt gatcggcctt gaggccgcgg
     5341 gaggaggcga tccggccgtg gatccgtgca cgcgcgtggc catcatggtc aaggacccgg
     5401 tcttcagcta cctcgatgaa gaggacaaca ccaagtggct tggcgacgtg gccaggaagg
     5461 aggttaacct ggtgaaggag gcgctggcga ccagcctgtc gctgcagcga gtgacgcagt
     5521 tctctcccca ggtagccagc gaggcgatca ccaaggcgat tatcgccgtg gtgctgtctc
     5581 ttatcgccat cgccgcctat ctgtggatcc ggttcgggtc gctgaccttc ggcctggcgg
     5641 gcattctcgc cctttatcac gacgtggcca ttgccctggc gggcattttc atcgcccacc
     5701 acgtctggca cacacgggtg ggcgaggtgc tcatgcttcg cgattttcgg atcgacctga
     5761 acatcgtcgc cgcccttctg accatcgtgg gcttctcgat caacgacacg atcgtgattt
     5821 ttgaccgcat tcgtgagaac cgcggccgta ttgcgacgat ctcagcccgg ctgatcaacg
     5881 actcattgaa ccagaccttg tcgcggacca tcctgacgac gttgaccgtg ttgatgacgc
     5941 tgttcgtcat gtatttcttc ggcggcgagg gcattcacgg tttcgctttc gcgatgatcg
     6001 tcggttcgct cagcggtacg tacagtacgt tggctatcgc cacgccgttg atccaccatc
     6061 cgcggatcct ttgggtggtg acggtagtgc ttgccggtct gacgcttgcc ggtctggcat
     6121 ggatgatccc cggccgcatg gtgtcgaggg tgttgccgct ggtgattctg gggctgtcgg
     6181 ttgtcgtgct ggtcgttttg gcccggagct tcgcccgaga ggaccgagca gcttcgcccg
     6241 cctgatttgc ggccggattc ggccttttga aaaacgaacg ggcctcggtt cgcctgaata
     6301 ccgggcggac cgaggcctgt tgcgctaaag cgtcatgtcc ggcgggtgat gccggttctc
     6361 acgtcccctc catgaccgtg aagtagacgc tgacggcgta atcaagcgtt tcttcgtcga
     6421 ggtggacggg gatcttgttg tactcgcagt agttcttgga aagcatgacc aggtcgcgcg
     6481 gctggcaaca gcggaaggga cgacccgttt gtgcatagtg tttgtcgatc agcttcgtga
     6541 ccagtgcgtc gtcgtacttg atcccgagga agtcgcacat catttgcatc agcttgcggt
     6601 attcttcctc agacgggttg tcgacgtgga tcttgtaggg gatacgcctc aggaaggcgt
     6661 cgtcgacgag gtccttcggc tcgaggttgg tcgagaagat caggagctga tcgaacggca
     6721 cctggatttt cacgccgttg gcgagggcca gaaagtcgaa gcgcttttcc agcggcacga
     6781 tccagcggtt gagcagttcg aaggggttca tgcgctgccg gccgaagtca tcgatcacca
     6841 gcgtgccgca gttgcttttg agctgcatcg aggcttcgct gacccgggtc gtgggattcc
     6901 agcggatttc gagctgatcc atggtcaatt cgccgccgac cacgacggtg gggcgtttga
     6961 ttcgcaccca tcgctcgtcg acggcggcgg tcttgaagat cgacgacttg ggtaacggaa
     7021 tccgctcatg aatctcgggg tcgaagagct tgacgatgaa gccgtcgatg tggatggcat
     7081 agggaatgta gatctcgctt ccgaaacagc gggtgatccg ctcggcaata ctggtttttc
     7141 cgtttccggg agggccatag aggaacaggc cgcgtccgga attgatcgcc ggtccgaggg
     7201 tcgagaacat cctttcgttg atgagcaggt cggaaaaggc gtttctgagc tgatcagggc
     7261 cgggcttctc attggtgatc gattgagcgg cgactgcctt gcagtaggcg tcgagcggaa
     7321 cgggggcggc gccgaaatac atgctctctt ccatgaactg ccgggctcgc ccgcggccgg
     7381 cctcggtcaa cgtgtaggtg aagtcgccca tgttggcgtt gcccacgtag ccgaggagtt
     7441 gttgcttctt gagatccgtc agccgttcga cgaccagcgg gtgaggcaac gccagcgtgg
     7501 aggcgatttt ggcgcccggt gccgaaccga cgttcatgag gtacttgaag atcagggcgt
     7561 cgaccatctc gttggtcaga ccggcctgct cgagggactc ggggggaacg ggttggtaga
     7621 tattcgtcgt tgcggaggac gccgtttgag ccggcggttc cgcgaccgca gcgttgctga
     7681 gtggcatcct gacctccttc agagcgataa aaactatcct cttgccattt gaacgcttgt
     7741 tgacaggcgc gctgtcatcc agaggctgcc tggtgggtca tcggtgatcc gaaggcgggc
     7801 cttgaagtcg gatggagacc cgataactaa caggcgcgaa gcgggcgggt tataatcggt
     7861 tcccgtctcg cggatttctg cgcggctgat atgcgtgcgg ctgggatgac gcccaacggc
     7921 ggacgtcctt ccccggtggt ggcggttatg aaagtacgga tgacgggggt gaggccgaga
     7981 tggtgatcag gctgcggagc gtgctcgttg cgatggtggt tgcctggccg gcgcttcttg
     8041 tgccgccggt cttgcgtgcc ggggacatgc ctgtcggcgg ggttcatgtg aagattctgc
     8101 ccccgctgcc cgtgtggccc tcggactacg agacgatcac cgagcgccgg ccgacatttc
     8161 acatctgcgg cctgtcttcc gccacgaagt accgcgtgga actggctcgc gacgccgctt
     8221 ttgccgacgg cttcaccctg actgaattcg agatcacgga cacctcgggc atctcgcccg
     8281 tggtgttggt ggcttaccag ggtgagcctc tgcccgacgg gcagtactac tggcgggcct
     8341 tttgcggcga cgacgagggg tttt
//
LOCUS       DAOTPW010000254         8296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7718_length_8296_cov_4.889455, whole genome shotgun sequence.
ACCESSION   DAOTPW010000254 DAOTPW010000000
VERSION     DAOTPW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7718_length_8296_cov_4.889455"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2033
                     /locus_tag="P5316_18210"
     CDS             <1..2033
                     /locus_tag="P5316_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86948.1"
                     /translation="RGVACGMGLALIVLAAHGVSIWDGLFFDDHWHRAAFRKYGWSPS
                     ELMESATIDLSGKLNHLWWQERPAVWRYPRPVAMAIAKAEFVFSDGDPRIIHACALCW
                     HWFMALLVYWLAFQAGLERRWAFCAGVLFIILPHSFFSLGWTAARNAVVGTFFFTAAV
                     AAYMSVSLGSPSRWFVTRWIREALPVLLWILAILSRETAVIFAFVIFLLDVSYGGRRH
                     FSRRLPYHAVFWVLTLAFVAWRWTSFDVGNVPDIYFTKLTGWKSGYWAASKLLQLLFS
                     QIFYTPMLMGLATYQGVAREELLAHGVMAVLVGLVAVWYVAVSKGKGGRWVWPLWCIA
                     AFAPVVPVFAMPHFSYLASVPYAIVVAILLAGVTAAWRKTVAFLVIGATVWSLGVYRL
                     AWWGIIRSEQIVYTDMVESTSRPPAPGGKLFFINLPIAAIYAPDAMREAWTTADLEGY
                     TLTFAPHPLMMTKPCVVEQLNDHEFTVSTEPPGYFSGLPGKMLIDGMRPGSPLTPGMV
                     IRGKPGFPFDATVVEADRSGVRKLRFTFHQPLATENYHFYLSSPERAAYRLTFNAAPT
                     AGDGGELFRRARSADLIEREAARWEIRDKAWPIAIAMGSPVQEYLSLQSESSLDRVEQ
                     WWAAYDIGRRFQEHVDWRSRNACVLRQRGLYFKVMDIVGRFVESDLYLTGRR"
     gene            2045..3436
                     /locus_tag="P5316_18215"
     CDS             2045..3436
                     /locus_tag="P5316_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86949.1"
                     /translation="MIPAPVNKTMTSVAFHTPNPLDLLFFAFDRAGARLSLPGAFIHA
                     HLEMAGQVDAEGMKRALAGIHRLYPATQARLECSALDGRPRWRLHCPPWDLNRLVRVH
                     HVKPATEAEIHHQVDALINQRIDLVTLPPLQFHVLRGLASGDWVVVRWPHAFMDFRGG
                     LIILDVMERLYQEGVDPLSLRSAGDELLDGLGRRLQAEERKTAPADELRPDMAGRPVD
                     LQLPPCPDFRNLGPLRTAVRRLDHRQCSIAQEISARSCAPARFGAYVRACAIQALHRA
                     LSESAGHGSVYSVPYILEGREPPYRSPVCHNVFSLDRLHVPVEMAPDRAAVARFLHEG
                     TAQMIQRSLVTGHLKRVLDITRLPPAILGAIVRHSLINPPSARGRGDFGAPPSLPMGF
                     SQAFGRDGRRFCGAELRYVHAFRPPLPRAGIGLQVVAEQGRLAICGLCYEVRWAMMNR
                     ILDDFVEALLNPG"
     gene            3487..4884
                     /locus_tag="P5316_18220"
     CDS             3487..4884
                     /locus_tag="P5316_18220"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HRR86950.1"
                     /translation="MSRRWVNALPRYQELNPLDLYFFAADQAMRRIGLPGTNIHLCLE
                     LNGRIDVDGLKRAVQALWRVYPATGSRLERSDVTGRPRWRLDSCPADASCVIRICELT
                     DASEHDIHGRIEALLNEPIDLRGLPPVRFVVLRGLAEGDRLVARWPHALMDGRGGVTL
                     LEELNRLYRESADHRSCASAGDESRRDFADLLREAPLVLKPREVLSSGTSLRPRDWRT
                     VRLSDGIRLDQLGPVRFMLRRLTAEQAREARAISLRVCGFARFGDFVRACAIRALDRV
                     APKPLPHNAGYCTLNLIDLRKRRQAAPVCRNLTNALPMYVPARIAQDRRAVADLARDQ
                     MAEMLQSRTMLLRLATVHRFTLLPTSLLVNVLHQRLIATGLRGMPTGMGNAPSLPLGL
                     IGSFSKGMSTFCGAELTNVYGLRPVPFQTGISVDVNEAQDRLNVAAMYYEPAISTAKM
                     AAFLDDFAASLVSME"
     gene            complement(5049..5225)
                     /locus_tag="P5316_18225"
     CDS             complement(5049..5225)
                     /locus_tag="P5316_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86951.1"
                     /translation="MPRKSKAFKSKQREAQKASRKGDTKKANEIWQQIIVDRVKLKAE
                     KAEKRAARKGRAVA"
     gene            complement(5524..7707)
                     /locus_tag="P5316_18230"
     CDS             complement(5524..7707)
                     /locus_tag="P5316_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86952.1"
                     /translation="MRATNVLIAFCACVTVPEAFGRQAAGPQPFMKAKTCFQTNTPYE
                     PRIALAVDSVIVHRHGEDPARLAGMIGSWKQHGYIVGRMFFADSDATNEYWTGKWDGI
                     PHPQEVERDEKGQVRMCAGIRPYMLPTEGWIRYLEEMAVESVNAGADAVLPEEPLAHV
                     DTGYEEMFRKLWVESYGVPWQPENASPEARYLTAQLKTDLYVKLQERLCQAVDRRSRE
                     LGRRVDFVVPIHSIYSNVASRLTAPLGTSLKIKGSDGYIGQIWTGPVNWAIWNYDSPD
                     KTFFDSAYCLYDFFVALASGGNRKLWLLVDPVEDDPNHKWSEFEEWYVHCTAAMLMFT
                     EVDSYEAMPWPERIFLKGHTTGGGTPAPERFRTIVLSATQVLQEVPLGGTWNPHLPQS
                     KTDTVRSVDGIGVAVSDTLMWEKEPPPSLQVAYGLMMPLVSAGVPASACILERMSEPT
                     YLSRFKVIVLSYEAFKPMEAQPNVALARWVKNGGVLVILGEADDLGGARLWWRKAGYP
                     SPLHHLMAELGLNDVDAAGESPVGKGWVLRRPTSPREFGRRKNVQEVYLPLLKQAWSR
                     ADSSRELPTPGYLCVKRGPFVIARAGREALRLPGTFVDVVDPRLAVLKDVEIKPGTSG
                     IYRDVTDIVEVTTAGSRKPKVLHATHRLVEERAGQDRLRIVIRGPLETPGLVRLFTAG
                     RRLSGVTARDLSGDGRDARWTQEDQTILISFSNEPAGLAVEAAWK"
     gene            complement(7709..>8296)
                     /locus_tag="P5316_18235"
     CDS             complement(7709..>8296)
                     /locus_tag="P5316_18235"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR86953.1"
                     /translation="SRVMLARLVWDNPQVLILDEPTNHLDIPAREALEESLIEYEGAI
                     LLVSHDRYFLDRVVNRLLVLPERGRYELMIGNWSTYEHRVAEREKERLEAEEEARKQA
                     RRDARMRARRAVKRAPPPSDGPVSPFVAWSLDRLEREIMDREEKLAATEAMFADPAVY
                     RDAERARALRGEVESLRAELAALNAEWERRIEEES"
BASE COUNT         1411 a         2699 c         2518 g         1668 t
ORIGIN      
        1 cgcgaggtgt cgcctgcggc atgggtctgg ccctgatcgt tctggcggcc cacggagtca
       61 gcatctggga cggtctgttc ttcgacgatc attggcaccg tgcggcgttc cgcaagtacg
      121 gctggagtcc ttcggagctg atggagtcgg cgacgatcga tctttccggc aagctcaatc
      181 acctctggtg gcaggaaagg ccggcggtct ggcggtatcc tcgcccggtg gcgatggcga
      241 tagccaaggc cgagtttgtt ttcagcgacg gcgacccaag gatcatccac gcctgcgccc
      301 tgtgctggca ctggttcatg gctctgctgg tgtactggct cgcgttccag gccggcctgg
      361 aacgacgttg ggccttttgt gccggggtcc tgttcatcat cctgccgcac tcgtttttca
      421 gccttggctg gacggccgcc cggaacgcag tagtgggcac attcttcttc acagcggccg
      481 tcgccgcgta catgtctgtg tcactgggat cgccatccag atggttcgtc actcgatgga
      541 ttcgcgaggc gctgccggtg ttattgtgga tcctggcgat cctcagccgt gagacggcgg
      601 tcatctttgc ctttgtcatt ttccttctcg acgtcagcta cggcggccgg cgacatttca
      661 gccggcgact tccttaccat gcggtcttct gggtgctgac gctggcgttc gtcgcctggc
      721 gatggaccag ctttgacgtc ggaaacgtgc ccgacatcta cttcacgaag ttgaccggat
      781 ggaagtccgg ctactgggcc gcgagcaagt tgttgcagtt gcttttctca cagattttct
      841 acacgccaat gctgatggga ctggccacct atcagggagt ggctcgcgaa gagttgttgg
      901 ctcacggggt tatggccgtc cttgtcggac tggtcgcggt ctggtacgtc gccgtctcca
      961 agggcaaagg gggccgctgg gtatggccgc tgtggtgcat cgcggcgttc gctccggtcg
     1021 ttccggtctt tgccatgccg cacttcagct acctggcgtc ggtgccttac gcgattgtgg
     1081 tcgccattct tctggcgggc gtgacggccg cgtggaggaa aacggtcgcg tttctggtca
     1141 tcggggcgac ggtctggtcg ctaggcgtct accggctggc gtggtgggga atcatccgct
     1201 cggagcaaat cgtgtacacc gatatggtcg agtcgacctc ccggcctccg gcgccgggcg
     1261 gcaagctttt cttcatcaac ttgccgattg ccgccatcta cgcaccggac gcaatgcgcg
     1321 aggcatggac cactgccgac ctggagggat acacgctgac gttcgctccg catccgctca
     1381 tgatgaccaa gccctgcgtc gtcgaacaac tgaacgacca tgagttcacc gtttccacgg
     1441 agccgccggg ctacttctcc ggcctgccgg ggaagatgct gatcgacggc atgcgaccgg
     1501 gctcgcccct gactcccggc atggtgatca gaggaaaacc gggttttccg tttgacgcca
     1561 cggtggtcga agccgaccgt tccggcgttc ggaagcttcg gttcacgttt catcaaccgc
     1621 tggccaccga gaactaccat ttctacctat cgtcgccgga acgagcggca tatcggctga
     1681 ctttcaacgc cgcgccgacg gccggcgacg gcggggagct gttccggcga gccagatccg
     1741 cggatctcat cgaacgcgag gccgcccggt gggaaatccg cgacaaagca tggccgatcg
     1801 caatcgccat gggctcgcct gttcaggagt atctctcgct gcaatctgaa agctcactgg
     1861 accgcgtcga gcagtggtgg gccgcctacg atatcgggcg gcgtttccag gagcatgtcg
     1921 actggcgttc gcgaaacgcg tgcgtgcttc ggcaacgcgg cctgtacttc aaggtgatgg
     1981 acattgtggg caggttcgtc gagtccgact tgtatctcac tggcaggaga tgatgccggg
     2041 ccggatgatc ccggcgcccg tcaacaaaac gatgacatcg gttgcgttcc acactcccaa
     2101 tccactcgac cttctcttct tcgcctttga ccgggcgggg gccagactgt cattgccggg
     2161 cgccttcatt cacgcgcatc tggagatggc cggccaggtc gacgcggagg ggatgaagcg
     2221 ggcgcttgcc gggattcatc gcctctatcc tgccacgcaa gcccgactgg aatgctccgc
     2281 gctggacggc cggcccagat ggcgactgca ctgcccgccg tgggacctca accgtctcgt
     2341 tcgcgtccat cacgtgaagc ccgcgaccga agcggagatc catcatcagg tcgacgcact
     2401 catcaaccag cgcattgatc tggtcacgtt gccgccgctg cagtttcatg tcctgcgggg
     2461 tcttgcttca ggcgactggg ttgtcgttcg ctggcctcat gcgttcatgg atttccgagg
     2521 cggcttgatc attctggacg tcatggagcg cctgtaccag gagggagttg accctctttc
     2581 tctgcgcagt gcgggcgacg aactgctcga cgggctcgga cgccgtctgc aggccgagga
     2641 gcggaaaacg gccccggccg atgagttgcg gcccgacatg gccggacggc ccgttgatct
     2701 gcaactgcca ccgtgccccg acttccgcaa cctggggccg ctgcgaaccg ccgttcgacg
     2761 tctggatcat cggcaatgct cgatcgccca agagatctcg gcgagaagct gtgctccggc
     2821 cagatttggc gcctatgtcc gtgcctgcgc gatccaggcc ttgcaccgcg cgctatcgga
     2881 atcggccggt cacggaagcg tctactccgt tccatatatc ctcgaaggcc gcgaaccgcc
     2941 ctaccgcagt cccgtctgcc acaacgtctt cagtctcgat cgcctgcacg tcccggtcga
     3001 gatggccccc gatcgcgcgg cggtcgcacg ttttctgcac gaaggtacgg ctcagatgat
     3061 ccagcgcagc ctcgtaacgg gccatttgaa gcgggtgctc gacatcacgc ggctgccgcc
     3121 ggcgattctc ggcgccatcg tgcggcactc gttgatcaac cctccgtcgg ccagggggag
     3181 aggcgatttc ggcgctccgc cttcgttgcc gatgggtttc tctcaggctt tcggccgcga
     3241 tggacggcgg ttctgcggcg cggagcttcg gtacgtgcat gcatttcgtc cgccgctgcc
     3301 tcgagccggc atcggtttgc aggtcgttgc cgagcaagga cggttggcga tctgcggctt
     3361 gtgttacgag gttcggtggg cgatgatgaa ccgcatcctc gatgacttcg tggaggcctt
     3421 gctcaatccc ggttgaacgg gggacgcgac ccttgtgaag gatgaacgag acgaccagaa
     3481 ccggcaatgt cacgacgttg ggtaaacgcc ttgccccgat atcaggaact caatccgctc
     3541 gacctgtact tcttcgccgc cgaccaagcg atgcggcgaa tcggccttcc gggcacgaac
     3601 atccacctgt gcctcgaatt gaacggtcgt atcgacgtgg acggtctgaa acgtgcggtg
     3661 caggccctgt ggcgcgtcta tccggccacg ggctcccggc tggaacgttc cgacgtgaca
     3721 ggccgcccgc gctggcggct tgatagctgc cccgccgacg cgtcctgtgt gatccggatc
     3781 tgcgaactga ccgatgcgag cgaacacgat atccacggcc ggatcgaggc cctgctcaac
     3841 gagccgattg atctgcgcgg cctgcctccg gttcggtttg tcgtgcttcg cgggcttgcc
     3901 gaaggcgacc ggctggtggc tcgctggcct catgccctga tggatggccg cggaggcgtc
     3961 accctgctgg aagagcttaa ccgcctttac cgcgagtcgg ccgaccaccg gtcttgcgcg
     4021 tccgcagggg acgagtcccg ccgcgatttc gcggacctgc tgcgagaggc cccgctggtt
     4081 ctcaaaccac gggaggtgct cagcagtgga acaagtctgc gtccaagaga ttggcgaacc
     4141 gttcgcctga gcgacgggat tcgccttgac cagctcgggc ccgtccgatt catgcttcgc
     4201 cggctgaccg ccgagcaggc tcgcgaggcc agggcgatct cgttgcgcgt gtgcggtttc
     4261 gcccgtttcg gagatttcgt gcgagcctgc gccatccggg cccttgaccg cgtcgcgccc
     4321 aagccgttgc cgcacaacgc cgggtactgc acgctgaatc tgatcgatct ccgaaaacgc
     4381 agacaggccg caccggtctg ccgcaatctg acgaacgccc tgccgatgta tgtacccgcg
     4441 cggatcgcgc aagaccgaag ggcggtcgcc gacctggcaa gggaccagat ggcggaaatg
     4501 ctgcaatcga gaaccatgct cttgaggctt gcgaccgtcc atcgattcac gctcctgccc
     4561 acgagcctgc tggtcaacgt cctgcatcaa aggctcatcg cgacggggct caggggcatg
     4621 ccgacgggca tgggaaatgc gccctcactg ccgctcggcc tgatcggttc attcagcaag
     4681 ggaatgtcca cgttctgcgg cgcggaactg acgaacgttt acggattgcg gccggtgccc
     4741 ttccagacgg ggatctcggt tgacgtcaac gaagctcagg accgactcaa cgtcgccgcg
     4801 atgtactatg aaccggccat cagtacggcg aagatggccg catttctgga cgactttgcg
     4861 gctagcctgg tgagcatgga gtagcgcacc gcaccggcag ccccttgtcg agccttccgg
     4921 tgtcaaagca cggctcgcag gttactcgcc tgattaacag gcctcacatc cgatgtgccg
     4981 caccggaaac cgaaggcgtg cagtcccggg cagagatcag aaacaatgct gcttttctac
     5041 cgggtcggtc aggctaccgc ccgccctttc ctggccgctc tcttctcggc cttctcggct
     5101 ttcagtttta cccggtccac gatgatctgt tgccagatct cgttcgcctt tttcgtgtca
     5161 cctttgcgac ttgccttctg ggcttctcgc tgcttgctct tgaatgcttt tgattttctg
     5221 ggcatcacga ctcctgattg cacacccgag tgcgcgttac gctcaccgtg agcgcgacgc
     5281 cactcgtcca cgaaccgccc gcatccacga atgaaaccaa tcccagtctg cgggcaaccc
     5341 gcggcgtacc cggttgccaa ctgattgagt tgtgatttcc cgaaaggcct gtgtgatcct
     5401 gccgtccggt ttcgtgcccc tattatagca cgcgaccctg ctttagccac ccccggaatg
     5461 ccgtccgaac gcaagaaccg ggtgcatgtc ggaggatgag ccgcgaaacg gtcgccgcga
     5521 ggatcatttc caggcagcct cgacggcaag accagcgggt tcattggaga agctgatcag
     5581 gatcgtctgg tcctcctgag tccaccgcgc gtcccgtccg tcaccggaga gatcgcgggc
     5641 ggtcacgcct gacaggcgcc tgccggccgt gaagaggcgc accaatccag gcgtctccag
     5701 gggcccacga atcacgatcc gcagccggtc ctgcccggcg cgctcttcga ccaggcgatg
     5761 ggtcgcgtgc agcaccttgg gtttgcggct cccggcggtt gtgacctcca cgatgtcggt
     5821 cacatcgcga tagatgccgc tggtaccggg cttgatttca acgtccttga gcacggccaa
     5881 ccgcggatca accacatcga caaacgtgcc aggcaacctc aacgcttccc ggcctgccct
     5941 ggcaatgaca aacggacctc gcttgacgca cagataaccc ggcgttggca gctctcgtga
     6001 cgaatcggcc ctggaccagg cctgtttgag caacggcagg tacacctcct gcacgttttt
     6061 ccgccggccg aactctcgcg gcgacgtcgg ccggcgcaat acccatcctt tgcccaccgg
     6121 cgattcaccg gccgcatcga cgtcgttcag tccgagctcc gccatcaggt gatgaagcgg
     6181 cgaagggtac cccgccttgc gccaccatag cctcgcccct ccgagatcgt ctgcttctcc
     6241 cagaatcacc agcaccccgc cgttcttcac ccagcgagcc agtgccacat tcggctgggc
     6301 ctccatcggc ttgaacgcct cgtatgaaag cacgatgacc ttgaagcgtg acaggtacgt
     6361 gggttcgctc attcgctcga ggatgcacgc cgaggcaggc acgccggcgc tgaccagcgg
     6421 catcatcaga ccgtaggcaa cctgaagcga aggcggcggc tccttctccc acatgagcgt
     6481 atcggacacg gcgactccga tcccatcgac tgaacggact gtatccgttt tgctctgcgg
     6541 caggtgagga ttccacgtgc cccccagggg gacttcctgg aggacctgcg tggccgacag
     6601 cacgatcgtc cggaatcgct ccggagcagg ggtaccgccg cctgtcgtat gccccttcag
     6661 aaagatccgt tcgggccagg gcatcgcttc gtacgagtcc acctccgtga acatgagcat
     6721 ggcggccgtg cagtgaacgt accattcctc gaactcgctc cacttatggt tcggatcgtc
     6781 ctcgacggga tccaccagca gccacagttt gcggtttccg ccggaggcca gggccacgaa
     6841 gaagtcatag agacagtagg ccgagtcgaa gaaagtcttg tccggcgaat cgtaattcca
     6901 tatcgcccag ttgaccggtc ccgtccatat ctgcccaatg tatccgtccg accctttgat
     6961 cttcaacgac gttcccaaag gggcggtgag ccgcgacgcc acgttgctgt aaatgctgtg
     7021 aatgggcacg acaaagtcga cccggcgacc caactcccgc gaacgacggt ccactgcctg
     7081 gcataaccgc tcctggagtt tcacatacag gtcggtcttc agttgggcgg taaggtagcg
     7141 ggcctcgggt gaggcgtttt cgggctgcca aggcacccca tagctttcga cccacagctt
     7201 gcggaacatc tcctcgtacc ccgtgtcgac gtgagccagg ggctcctccg gcaacacggc
     7261 gtccgctccg gcgttgaccg attcgaccgc catttcctcg aggtagcgaa tccagccctc
     7321 agtcggaagc atatacggtc gaatgccagc acacattcgc acctggccct tttcgtcgcg
     7381 ctcgacttcc tgcggatgcg ggattccatc ccacttgccg gtccaatact cgttggtcgc
     7441 atcggaatcg gcgaagaaca tgcgaccgac gatatagccg tgctgcttcc acgagccgat
     7501 cattccggcc agcctggccg gatcctcgcc atgccggtga acaatgacgc tgtcgaccgc
     7561 caaagcgatc cgcggctcat agggcgtgtt ggtctggaag cacgtcttgg ccttcatgaa
     7621 cggctgcgga cccgcggcct gccggccgaa cgcctccgga accgtgacac acgcgcaaaa
     7681 agcgatcagc acattggttg ccctcacgtc acgactcctc ctcgatccga cgctcccatt
     7741 ccgcgttgag agccgcaagc tcggcgcgca gcgactcgac ctcgccacgg agggcccgtg
     7801 cccgttcggc atcacgatac acggccggat cggcaaacat ggcctcggtc gcggccagct
     7861 tctcttcccg gtccatgatc tcccgttcga gccggtccag cgaccaagcg acaaacggcg
     7921 acaccggacc gtcagagggg ggcggggctc gcttcactgc ccgtctggcg cgcattctgg
     7981 catcccgtcg ggcctgcttg cgggcctctt cctcggcctc aagacgctcc ttctctcgct
     8041 cggcgacccg gtgctcatag gttgaccagt tgccgatcat cagttcgtac ctgccgcgct
     8101 ccggcagcac aagcagccgg ttgaccacgc ggtcgaggaa gtagcggtcg tggctgacca
     8161 gcagaatcgc cccttcgtat tcgatgagcg attcttccag tgcctcgcgg gcggggatgt
     8221 ccagatggtt ggtcggctca tccaggatca gcacctgggg gttgtcccag accaatcggg
     8281 ccagcatcac ccggct
//
LOCUS       DAOTPW010000255         8234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7781_length_8234_cov_5.427681, whole genome shotgun sequence.
ACCESSION   DAOTPW010000255 DAOTPW010000000
VERSION     DAOTPW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7781_length_8234_cov_5.427681"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="P5316_18240"
     CDS             complement(<1..843)
                     /locus_tag="P5316_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRR86954.1"
                     /translation="MPDLAKAGIGTRCLHAGQDPDPTTLSRAVPIYATTSYVFKDTDH
                     AANLFALKEFGNIYSRLMNPTNDVLEKRMAALDGGVGGLAVSSGQSAIYIAIFNICHA
                     GQNFISATSLYGGTWTLFTQTFPKLGITPRFFDPSRPEEIRELADANTRCVYIESIGN
                     PKNDVPDYAEIAKVAHSLGLPVICDNTVTTPVLLRPFDHGVDINIYSCTKFIGGHGTH
                     VGGCIVDSGKFDWTADRKKWPEFCEPDPSYHGVVFADALKPIGNIAYIIKCRTHFLRD
                     MGSCM"
     gene            complement(1058..2818)
                     /locus_tag="P5316_18245"
     CDS             complement(1058..2818)
                     /locus_tag="P5316_18245"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRR86955.1"
                     /translation="MDSLFRHRRLSAGLPIVAAFAVLVDRPVVARANRGAPWAMHVID
                     NSLSGADGVRLADVNGDGLPDITTGWEEGNAVRAYIHPGHAAVTTAWPAVTVGQAGST
                     EDAVFVDLDDDGAVDVVSAGQGSQTLFVHWAPANPGDYLNPGLWETVKIPASIGHGWM
                     FTVPMDVDGSNGIDLVSGGEGTAPKIAWFECPAGNRRNLAAWNMHVMSEVAWTMSLIP
                     YDVDNDGDGDVIVTDRYNNAGLQGARWLENPGTGSPLQMSPWPNHFIGAQGKQAMLSV
                     IEDLDRDGLDDLIVPTQGVGGLSFFRRTDRVSNSWKEYPIAKPPDTGAAKGCNVGDID
                     LDGDPDIVFSFAAAASPLSGMVWLSYANAPTDPVWQDHEISGPAGEKFDIVALADLDG
                     DGDLDVITTEENEPPNSRGLGVIWYENPTITPLDTDHDGVPDASDNCLLVPNPSQEDL
                     DEDDIGDACDDDVDGDGALNTGDNCPTTSNADQADADADMVGDVCDHCAGTIPHSPVD
                     ATGCPPPIPGDCDRDGDVDQKDFGHMQACLSGRGAVQALPDCQKAHLDADDDVDEADM
                     LVFHRCISGPDVPADPGCAE"
     gene            complement(2934..3653)
                     /locus_tag="P5316_18250"
     CDS             complement(2934..3653)
                     /locus_tag="P5316_18250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR86956.1"
                     /translation="MKISAGVLLGFGLLSLNLAHADFVAYNDLAGFSPPNATSIGLNG
                     SGLLKDYDTGTNTSVMLQVSGYLVSTLSKPEVPDYFGGGDALAEFDQKIISDDFIKES
                     ADSPDAWVNLDFSGLDPNKLYTVVVTGNRGANPNRTAKFAISGVDSFVNQSSIGAPWI
                     GPDWVEFDTGVNTDTGYVAKFADIRVGSDGQMRVTVTPGPHNGDRNKFFLNQLKLTEI
                     PEPGALGMLCLGGLLLMLRRR"
     gene            complement(4066..5013)
                     /locus_tag="P5316_18255"
     CDS             complement(4066..5013)
                     /locus_tag="P5316_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86957.1"
                     /translation="MRSIPISFLCVLFCLSGKAAGEFTAYNDLAGSSSGNVTAINLSQ
                     SGLLKDYETGMDTPVTLTVEGSEVASDTKSVDDFQDGDALVEFGTIVNNDGYIRHRDQ
                     PAGNPNSWVTCSFSGLNPNRLYTVVITGSRGTLANAYPSRTAIFSISDVESFVNESSA
                     GAPWRGEDWVEFNTGHNSYQNEGFVARFTKIRCGNDGDMTIRVAPGPHNAQIDPADIG
                     KWYLNQLKLVESGMPCNTPFADSDGDGDVDQADFAVFQMCLTGVGGGVPAGCECFDRD
                     ENGAGDGDIDQTDWDRFEACATGPGIASDPECEIARAQP"
     gene            5638..>8234
                     /gene="alaS"
                     /locus_tag="P5316_18260"
     CDS             5638..>8234
                     /gene="alaS"
                     /locus_tag="P5316_18260"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRR86958.1"
                     /translation="MEYRTADQIRQEFIAFFEGRGHTVVPSSPVVPIDDPTLMFANAG
                     MNQFKDVFLGTGSRPYKRAVDTQKCIRVSGKHNDLEEVGRDTYHHTFFEMLGNWSLGD
                     YFKREAIAWAWELLTEVWGLPKDRLYATVFGGDPEEKLAADDEAARLWTEVTDIDPAH
                     VKRFGKKDNFWEMGVTGPCGPCSEIHIDLTSDGSGESLVNSGDPRVMEIWNLVFIQFN
                     RDESGKLSPLPARHVDTGMGFERIVAVLQGKRSNYDTDVFAPLINRICELTGHSYTAR
                     LDSNTDIAIRVIADHARMLTFAITDGAKPSNEGRGYVLRRLLRRAARFGRQQLGRHEP
                     FIYKLVPTIVQTMGHVFPELKERPEKVAELIRDEEAGFGRTLDRGIALFEEAAAGGKS
                     ISAEYAFKLYDTYGFPIDLTVQMAGERGIRVDEAGFVRLMSEAKEKARAGAKKHVMVA
                     IDGELPVTDDAPKYDGRQTEAKIVGWVKNNALVCSGSLSPDDGEVAVVLDRTCFYAEQ
                     GGQVGDAGKIITGTGVFDVAITEKLGDGILHIGTVTDGRIEAGQSARLEVDAARDLTR
                     KNHTATHLLHWALCRVLGDHVKQHGSVVDPDRLRFDFDHGSQIAHEDLQEVERLVNEK
                     VCADLPVTTRVLPIDEARKLPGVRAFFGDKYGDTVRVIIIGDGFSTEFCGGTHLSRTG
                     EIGFFKILGEEGIAKGVRRITAATGPRAVEAVQQLERTARQVAGILKAGYDQLPDRIA
                     GLQEEIKKLRRQLAKGAAADVKSVRQELLDKAERAGGTAIIVGELPEVPIEQVREAAD
                     WLRTQAGSAAVCLAVCNDGKPMLIAAMTDDLVKKGLKAGELIQNIVPAIEGRGGGKPN
                     LAQAGGVNPG"
BASE COUNT         1617 a         2518 c         2521 g         1578 t
ORIGIN      
        1 catgcaactg cccatgtctc gcaggaagtg cgttcggcac ttgatgatgt acgcgatgtt
       61 gccgatcggc ttgagggcat cggcgaagac cacgccgtga tacgacgggt cgggctcgca
      121 gaactcgggc cacttcttgc ggtcggccgt ccagtcgaac ttgcccgaat cgacgatgca
      181 gccgccgacg tgcgtgccgt ggccgccgat gaacttcgtg caactgtaga tgttgatatc
      241 cacgccgtgg tcgaagggtc gcagcaagac cggcgtcgtc accgtgttgt cgcagatgac
      301 cggcaggccg aggctatggg cgaccttggc gatctcggcg tagtcgggca cgtcgttctt
      361 ggggttgccg atcgattcga tgtacacgca gcgggtgttg gcgtcggcca gctcgcggat
      421 ctcctccggc cgcgatgggt cgaagaacct gggcgtgatc cccagcttgg ggaaagtctg
      481 cgtgaacagc gtccaggtcc cgccgtagag gctggtcgcc gagatgaagt tctgcccggc
      541 gtggcagatg ttgaagatcg cgatgtagat cgccgactgc ccgctggaaa ccgccaagcc
      601 gccaaccccg ccatccaacg cggccatgcg cttctccagg acgtcgttcg tcgggttcat
      661 cagacggctg tagatgttgc cgaactcctt gagcgcaaag aggttggcag catggtcggt
      721 gtctttgaac acgtagctgg ttgttgcgta aatgggcacc gcccgggaca gcgtcgtcgg
      781 gtccggatcc tgtccggcgt gaaggcatct ggttccaata ccggccttgg cgaggtctgg
      841 catcgtgtca ctccttgtca aactggactc ccggaatcac cagccgtgcg catccaagat
      901 attgccctga acagagttaa agcgaaaagg gtctcggctg cgggctacat cgttcggtgc
      961 cgcatcatcg acccgcgatg agacaatata ggcccgcgcg attcagcaga ccagttgcag
     1021 cacctttcgg ctatgcgtaa cgggtgaccg acagacttca ttccgcgcag cccggatcgg
     1081 ccggcacatc gggaccgctt atgcatcggt ggaagacgag catatccgcc tcatctacgt
     1141 cgtcatccgc gtcgaggtgc gccttctggc aatcaggcag tgcctgcacg gcgccccggc
     1201 cactgaggca ggcctgcatg tggccaaagt ctttctgatc gacgtcgcca tcccggtcgc
     1261 agtcgccggg gatggggggt gggcatccgg ttgcatcaac tggtgaatga gggattgtgc
     1321 ctgcgcaatg gtcacagaca tccccgacca tgtccgcatc agcgtccgct tgatcagcgt
     1381 tggacgtggt ggggcaattg tcgccggtgt tgagtgcgcc gtcgccgtcg acatcatcgt
     1441 cgcacgcatc accgatgtca tcctcgtcaa gatcctcttg agaagggttg ggaacaagca
     1501 gacagttgtc agaggcgtca ggcactccat cgtggtccgt gtccaaggga gtgatcgtgg
     1561 ggttctcata ccagatcaca cccagaccgc ggctgttggg cggctcgttc tcttcggtag
     1621 tgatgacgtc gagatcgccg tcgccgtcca gatccgcaag agcaacgatg tcgaattttt
     1681 cgcctgccgg tccgctgatt tcgtgatcct gccagacagg atcggtggga gcgttagcgt
     1741 aggaaagcca taccatgccg gacaagggac tggcagccgc ggcaaaggaa aacacgatgt
     1801 ccggatcacc gtcaaggtcg atatcgccga cgttgcagcc cttggcggcg ccggtatcag
     1861 gaggtttggc tatgggatat tccttccagg aattcgagac ccgatctgtt cgccggaaga
     1921 aagaaagccc cccgacgcct tgcgttggga caatcaggtc atccagtcca tcacggtcga
     1981 gatcctcgat cacactgagc atggcctgct tgccttgtgc accgatgaaa tggttaggcc
     2041 acggactcat ctgcagcggg ctgccggtgc cgggattctc cagccagcgg gcaccttgca
     2101 ggccggcgtt attgtaccgg tcggtgacga tgacgtcgcc gtcgccgtcg ttgtcgacgt
     2161 cgtagggtat gagcgacatg gtccaagcga cctcggacat gacgtgcatg ttccaggcgg
     2221 ccaggttgcg ccggttgcct gccgggcatt cgaaccacgc gatcttgggc gcggtaccct
     2281 cgccgccgga caccagatcg attccgttac tcccgtccac atccatggga acggtaaaca
     2341 tccacccatg gcctatcgac gctgggatct ttactgtttc ccacagaccg gggttcaggt
     2401 agtcgcccgg gttggcgggg gcccaatgga caaacaacgt ctggctaccc tggccggcgc
     2461 ttaccacatc gacggccccg tcgtcgtcca agtcgacgaa aacggcatct tcagtgctgc
     2521 cggcctgtcc aacggtaacc gccggccagg cggtcgtgac cgccgcatgg cccgggtgta
     2581 tatacgcgcg gaccgcgttg ccctcttccc atcccgtggt gatatccggc aggccgtcac
     2641 cgttgacgtc tgcgagccgc acgccatccg ccccggagag ggagttgtcg atcacgtgca
     2701 tggcccacgg agccccgcgg ttggctcgag cgacgacggg ccggtcgacc aggactgcaa
     2761 acgcggcaac aataggcaaa ccggctgaca ggcgacggtg gcggaacagt gagtccattt
     2821 gtcagtttcc ctgcatcaga caagcccaag cgatgcctga ccatgcatga aaagggccgc
     2881 ccggttcaga cggccccttt cggtcgttgt tgttgcggcg cggcgccccc accctaccga
     2941 cggcgaagca tcaggagcag gccgccgagg caaagcatcc caagggcgcc cggttccggt
     3001 atttccgtca gcttcaactg gttgaggaag aacttgttgc ggtccccgtt gtgtggtcct
     3061 ggggtcacag tgacgcgcat ctgtccatcg ctgcccacac ggatgtcggc gaatttggcc
     3121 acatagcccg tgtccgtatt gacgccggtg tcaaactcaa cccagtccgg gcctatccag
     3181 ggagcaccga tcgagctctg attgacgaaa gaatccacac ccgagatcgc gaacttcgcc
     3241 gtgcgattgg ggtttgctcc ccgattgccg gtcaccacca ccgtgtaaag cttgttcgga
     3301 tccaagccac tgaagtccag attgacccaa gcatccgggg aatccgcaga ttccttgatg
     3361 aaatcgtcgc tgataatctt ctggtcgaac tcagcgaggg cgtcaccccc gccaaagtag
     3421 tcaggcactt cgggcttgct tagcgtggaa accaggtatc cgctcacctg gagcatgacg
     3481 ctggtatttg tgcccgtgtc gtagtccttg agcagtcctg agccattaag accgatactc
     3541 gtggcgttgg gcgggctaaa ccccgcgagg tcgttgtacg caacaaagtc ggcgtgggcc
     3601 aggttcaagg acaacaagcc aaaacccagt aatacgcctg ccgagatctt catattctcc
     3661 tcctttcaag gcaaagccgc atacaccgcc ggccacgcgg ccacgaaatc gctcctacgt
     3721 gctccacaga tgtgtaacat ccgatcttcc agcgctcagg ctgcatcagc ttcttgcctg
     3781 gtgaccttgc gctttggccg ctcgccgggc aggcgtgcga gttgtagccg gccagacagg
     3841 acacccccgt cggccacgac tatcagggta atagcgcctt ctgagcacac aagatcggac
     3901 gcgattatcc taccgcgccg ttgctcccac tgcaatctat ttctaagaaa agagcggatc
     3961 tggcaccccc ccacaaccct gggtcgcgaa tcgtaacccg ttgctggtca ggcatctgcg
     4021 gaatcgggcg ttcgccttgc caaaccggca tcgcgggttg ccggactacg gctgggcccg
     4081 ggcgatctcg cattcaggat cggaggcaat gccagggccc gtggcacaag cctcgaaccg
     4141 atcccagtcc gtctggtcga tgtcgccgtc tcccgccccg ttctcgtcgc ggtcgaagca
     4201 ctcgcacccc gccggcaccc cgccgccgac gcccgtcagg cacatctgga aaacggcaaa
     4261 gtccgcctga tcaacatcac cgtcgccgtc agagtcggca aaaggcgtgt tgcatggcat
     4321 gccgctttcg acgagcttga gctgattcag gtaccatttt cctatgtcag cggggtcgat
     4381 ttgagcgttg tgcggcccgg gggccacccg gatcgtcata tccccgtcgt taccgcatcg
     4441 gatcttcgta aacctggcca cgaacccctc attctgataa ctattgtgac cggtgttgaa
     4501 ttcgacccag tcctctcccc gccagggtgc gccggcagaa ctctcattga cgaaggactc
     4561 aacgtcagag atgctgaaaa tggccgtgcg gctcgggtac gcatttgcca gggtgccgcg
     4621 gctaccagta atcaccaccg tgtaaaggcg attgggatta agcccgctga aggagcatgt
     4681 cacccaggag tttggattac cggccggctg atcgcggtga cgaatgtaac cgtcgttgtt
     4741 gacgatcgtt ccgaactcga cgagtgcgtc accgtcctga aaatcgtcta cgctcttggt
     4801 atcagatgcg acctcagagc cttccacggt cagtgtgacc ggcgtatcca tcccggtttc
     4861 gtaatctttg agcaggcctg actgcgagag attgatggcc gtcacattcc cggaactgct
     4921 tcctgctaga tcattgtaag ccgtaaactc gcccgctgcc ttgccggaga ggcagaacag
     4981 tacgcacagg aaggagatcg gtatgcttct catcgtttcg cctccggaga aatatgccgt
     5041 atcgccgaga tggcataaga gatcatcgaa gagctcgata cacgatgagt gtatctcgcc
     5101 cttctcaaca acgcaagacc gtatgcgttg ttctcggttg ttttggcacg gatattctta
     5161 gctgaaccac cgctctgccg gcaacctcag agtcctggcg ataaagccgg agacgggttt
     5221 gcgcaaaagt cgtcaccgca ccacagccgg aatccccggc tccttcggcc cgattgtaga
     5281 tcaaagcgtc cttccacttg cgaaaacagc gccggacggt tgcttgatgg aagaaagcgg
     5341 gctgggggcc ctgggcggat gggtcgtagg gtcggcgggt cttgccgctt cagctcgacc
     5401 accgaggcac ggtgttgccc cgaccggtgg ttagccctga cccgaacaag cccacgaaag
     5461 gctacgcatc tgccgaggac accaaaccgt cgtccgcctg aggtcccact tcatgatccg
     5521 gcttcgttcg gctagaatac cctgtcccgg cacccgggaa cccctgacga gggcgaggct
     5581 tacggctgct tggcccgccg ggtttccagg gcggttttcg ccggacagga aacattcatg
     5641 gagtatcgaa cggcagacca gatacgacaa gagtttatcg ccttcttcga gggcaggggc
     5701 cacactgtcg ttccgtcctc gccggtggtg cccatcgacg acccgacgct catgttcgcc
     5761 aacgcgggca tgaatcagtt caaggacgtt ttcttgggta ccggcagccg gccctacaag
     5821 cgggcggtcg atacgcagaa atgcatccgg gtttccggca agcacaacga cttggaggag
     5881 gtcggccgcg acacctacca ccataccttc ttcgagatgc tcggcaactg gtcgctgggc
     5941 gactacttca agcgcgaggc cattgcctgg gcgtgggaac tgctcaccga agtgtggggc
     6001 ctgcccaagg atcgcctcta cgccaccgtc ttcggcggtg atccggagga gaagctcgcg
     6061 gccgacgatg aggccgcccg gctctggacc gaggtgaccg atatcgatcc ggcccacgtg
     6121 aagcgattcg gcaaaaagga caacttctgg gaaatgggcg tcaccggacc atgcggcccg
     6181 tgcagcgaga ttcacatcga cctgacgtcc gacggctcag gagagagtct ggtcaactcc
     6241 ggcgaccccc gggtcatgga aatctggaac ctggtgttca tccagttcaa ccgcgacgaa
     6301 tccggcaaac tgtcgccctt gccggccagg cacgtggaca ccgggatggg cttcgagcgg
     6361 atcgtcgccg tgctccaggg caagaggagt aattacgaca ccgacgtgtt cgctcccctc
     6421 atcaatagaa tctgcgagtt gaccggacac agctataccg ccaggctcga ttcgaacacg
     6481 gacatcgcta ttcgcgtgat cgccgatcac gcccgaatgc tgactttcgc gatcaccgac
     6541 ggcgccaagc ccagtaacga aggccgcgga tatgtcctgc gccgcctgct gaggcgcgcg
     6601 gcccgcttcg gtcgtcaaca gctcggccgg cacgagccgt tcatttataa gctcgtgccg
     6661 accatcgtgc agaccatggg gcacgttttc cccgaactga aagaacgtcc ggagaaggtg
     6721 gccgaactga ttcgcgacga agaagccggc ttcggacgca cgctcgatcg tggcatcgct
     6781 ctgttcgaag aggctgccgc cggcggcaag tcgatctcgg cggaatacgc cttcaagttg
     6841 tacgacacct acggctttcc gatcgacctg accgtccaga tggccggcga acgcggcatc
     6901 agggtcgacg aggcgggttt tgttcgtctg atgtccgagg ccaaggagaa ggcccgggcc
     6961 ggggcgaaga agcacgtgat ggtggccatc gacggcgaac tgccggtcac cgatgacgcc
     7021 ccgaagtacg acgggcggca aaccgaggct aagattgtcg gctgggtgaa gaacaacgcg
     7081 ctcgtctgct cgggctcgct gtcgcccgac gacggcgagg tggccgtggt actggatcga
     7141 acctgtttct acgccgagca gggcggacag gtgggcgacg ccgggaagat catcaccggc
     7201 accggcgtgt ttgacgtggc gatcacggag aagcttggcg acggcatcct tcacatcggc
     7261 acggtcaccg acggccgaat cgaggccggt cagtcggccc ggctcgaagt tgacgccgcc
     7321 cgagatctga cccgcaagaa ccacactgcg acgcacctgc tacactgggc tctctgccgg
     7381 gtgctcggcg accacgtgaa acagcacggg tcggtggtcg atccggaccg cctgcgattt
     7441 gacttcgacc acggcagcca gattgcgcat gaggatcttc aggaagtcga acgactggtc
     7501 aacgagaaag tctgcgccga tctgccggtg accactcgcg ttttgccgat cgacgaggca
     7561 aggaagctgc cgggggtgcg ggcgtttttt ggcgacaagt acggcgacac cgttcgcgtg
     7621 atcatcatcg gtgacggctt ctcaaccgaa ttctgcggcg ggacgcattt gtcccggacc
     7681 ggcgagattg gtttcttcaa aatccttggt gaagaaggca tcgccaaggg cgttcgccgt
     7741 atcaccgccg ccaccggccc gcgagcggtc gaggcagtgc agcagcttga aagaaccgct
     7801 cgccaggtgg ccggcatcct gaaggcgggc tatgaccagt tgcccgatcg tatcgcggga
     7861 ctccaggaag agatcaagaa actgcgccgg caactggcca agggagcggc cgctgatgtc
     7921 aagagcgtcc gacaggagct tctggataag gccgaacggg ctggtggaac tgcgatcatc
     7981 gtcggcgaat tgcccgaagt accgattgaa caggttcgtg aggccgccga ctggctgcgg
     8041 acccaggccg ggtcggccgc ggtctgtttg gcggtctgca acgatggcaa gcccatgctt
     8101 attgccgcca tgaccgacga tttggtcaag aaaggtctca aggccggcga gttgatccag
     8161 aacatcgtcc cggccatcga aggccgcggc ggcggcaagc ccaacctcgc ccaggccggc
     8221 ggagtcaatc ccgg
//
LOCUS       DAOTPW010000256         8154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7877_length_8154_cov_5.636375, whole genome shotgun sequence.
ACCESSION   DAOTPW010000256 DAOTPW010000000
VERSION     DAOTPW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7877_length_8154_cov_5.636375"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..241)
                     /locus_tag="P5316_18265"
     CDS             complement(11..241)
                     /locus_tag="P5316_18265"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRR86959.1"
                     /translation="MARMTFLGLAEKILREENKPLSPSEIWNIAMSKGYDEQLDSKGK
                     TPAATLYSAIFTNARTDPECQRRSESAEISPV"
     gene            1251..2537
                     /gene="lysA"
                     /locus_tag="P5316_18270"
     CDS             1251..2537
                     /gene="lysA"
                     /locus_tag="P5316_18270"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRR86960.1"
                     /translation="MDLFTYRNGELYCEDLPVRQIVDSGIGTPTYIYSATTFREHYSR
                     MASAFAELDPIICYSIKCCQNIHIVRLLVEQGAGMDVVSGGELYRALKAGADPSKIVY
                     AGTGKTDKEINEAIDARIGWFNIESEAELANLIHIASQRQTTVRAALRVNPDVDPKTH
                     RHTTTGKKETKFGVDIERAKRAFEEYGRNKSVRLCGIHLHIGSPVNAVQPYVEAIEKA
                     LDLILSLRRDGFEINTLDIGGGYGADYSAGEALSGKAYAQMIVPLLREQGLKIILEPG
                     RSIAANAGILVTRTLFIKRSGDRQFVIVDAGMSDLIRPALYDAYHFLWPVAPGPQYAL
                     AERRRNVNIPETVKVDVVGPICETGDFLARDRDLPPIKRGDLLAVFTAGAYGFTMASH
                     YNSRPNAAEVLVEGSTFRTIRRRETYDDLIAAEEQV"
     gene            complement(2501..3208)
                     /gene="rrtA"
                     /locus_tag="P5316_18275"
     CDS             complement(2501..3208)
                     /gene="rrtA"
                     /locus_tag="P5316_18275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HRR86961.1"
                     /translation="MKAADPATVNVHVTRVPGGTVLPGTISWERFFWIGVAAFSLLVH
                     LSPAVQSMLEFDRGHIAAGQTWRLLTGNFVHYSWAHLAANLMAFAALCWIAEGRSRGL
                     SWVVALSSPAVGLAVFGLADDVATYRGVSGVDCALLAWILTTMAIQDGRRKAMAWIGV
                     LALVAAKSVIEALTGQVLLPTSAPAGVALVGATHVVGLAIGAAAGLAGKRLEKRPRPG
                     GAAGSGQTCSSAAMRSS"
     gene            complement(3205..5295)
                     /locus_tag="P5316_18280"
     CDS             complement(3205..5295)
                     /locus_tag="P5316_18280"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HRR86962.1"
                     /translation="MNGSKHRRMGGSIAAGCMILMIQTAASGAGLLIADGGLGGVLEI
                     REHNVQVTINNGIAVTEVTQVFRNTENRQVEALYTFPVPKDASVSNFSMWINGKEMVG
                     EVVEKQRAREIYDSYKRVRRDPGLLEQKDFKTFEMRIFPIGPKADQKVQICYYQELDY
                     DHDWATYVYPLATQTRKDINSKTTGRFSFALQARSQVPIVEVESPSHGDQMAVARHGE
                     TQIQASLETKSGDLNRDVVVACHVSRPHTGIDMIASRRPGEDGYFCLTLTAGEELAKE
                     TSGMDYVFIIDSSGSMADDGKLALSRNSVEAFIRSLGKDDRFEIITFNVSPHSLFKSL
                     TTTGEESTAKASSFLASQEARGGTMMKPALEVAYKYADPARTLNVVIFSDGMTEQAES
                     RTLLEMIKSRPGNARVFCVGVGNEVNRPLLEQMADDSGGLASFLSPGDNFERQAQSFR
                     RKLTRPAATNIRVSIEGVETFDVEPPLVPNLFHGAPARMYGRYRGQGPGKAVVRAAVE
                     GQEIVSSVDLKFPDAEDANPEIERMWAWHKVQRLLKEGDRAGSHAAVANEIVRLGEAY
                     SIVTPYTSFLVLENDAEYQRWKIDRRNVLRSQRDRDSQQKLIADFEAIRNKAMTDLGP
                     RDGGVEPLTMTPPTAPLGGQQMVSTGAPPPPPSGPGDISLRPRGGGPVGPLGVAGIAF
                     LAVMEWRRRRRGAA"
     gene            complement(5469..6128)
                     /locus_tag="P5316_18285"
     CDS             complement(5469..6128)
                     /locus_tag="P5316_18285"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR86963.1"
                     /translation="MDGKPLTVQQQNVLDYISGFSERHGYPPTLREIGAALGLANVNA
                     VRGHVEALEKKGHITRTRDKARSIQLVHRPSAMSHVKRKLHEVFKTDEDVVHRVVYGL
                     AWVTWHRLPLLAGPRAEWMRHAFEREAIEHGWSIIECQIEPDHVVLVVETWPNHSPEK
                     TVHRFQSAGKAVRRKHPNDFPSESLWAKGYAATTSLDQLESMVASLLSKQGPPQRMEG
                     S"
     gene            complement(6270..>8154)
                     /locus_tag="P5316_18290"
     CDS             complement(6270..>8154)
                     /locus_tag="P5316_18290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR86964.1"
                     /translation="LVVMSHRARQMLLDVYHVPAERVALIPHGIPDVPFVDPNYYKDQ
                     FKAEGRRVILTFGLLSPNKGIEYMIEAMPEIVKAFPDVLYIVLGATHPHLVRQFGEAY
                     RSSLQRRVADLGLQEHVRFRNQFVELEELCEYIGAADVYVTPYLHEAQITSGTLAYAV
                     GAGKAVVSTPYWHAQELLADGRGVLVPFKDSKALADAVIHLFQNDTERHQIRKRAYTY
                     CREMVWKQVAREYLLLGQSLLHERARQPRLLAHKRRRVARAEELPEVDVRHLRTMTDP
                     TGVLQHCLYSTPDRRQGYSLDDNGRGLVVCALHRQLYDSDEVDDLAGIYLAYLSHAYN
                     EKTNRFRNFMSYSRQWLDAVGSEDSQGKALWGLGMTAAHAPNENSRMMAVQLFQDVIG
                     SAEQFEYPRAWAYSLLGIHAYLEYFSGDARVRRVRQVLAEKLFDLFRRNVSPDWQWCE
                     PTMTYSNATLVHALILAGTWIPDGEMRDQGLRSLQWLCDVQGSDRGHFSFVGNQGWFS
                     RGGEKATFDQQPIEASQMVLACAEAYRATREEKWLAKARLAVEWFLGRNDLDAPLYDF
                     RTGGCCDGLTPEGPNHNQGAESTLAWLIALLSFLIQVGRQTLDLKQDEEPAGWAEEQA
                     STR"
BASE COUNT         1518 a         2739 c         2304 g         1593 t
ORIGIN      
        1 gactacatat tcaaaccggc gaaatctctg cactttcaga acgacgctgg cactcgggat
       61 ccgtccgcgc gttggtgaag atggccgagt acagcgtagc cgccggcgtc tttcctttgg
      121 aatcaagctg ttcgtcatag cccttggaca tcgcgatatt ccatatctcg ctaggcgaaa
      181 ggggcttgtt ctcctcgcgg agaatcttct ctgcaagacc aagaaatgtc attcttgcca
      241 tgatgcacct ctttgcgatt actggcctga ggaacggcaa cacaagaaaa ggtcaggttt
      301 gttttctcct tcagtctatc acccgccgtc tgtcatctcg tcgattgcgt tcaccgctct
      361 ccccgtaacc tgaggcccgc cgtcggcacg atcacaggtt ataacctatc cccacagttt
      421 aacagataca agtggcaaga caagccgttg gctctcagct cttggctctt agctttccct
      481 tgctgtccct cgcccccttc ggcatagtcc gcgtcgtccg cggttcggtc ccgaacatcc
      541 gtttcctcct catgccctat cgcctttctc gtggccaaca acctccaccc cccatccgcg
      601 tccatctgtg tgatctgcag gccatacgcg ccgttggctc tcacgtttcc gggttcggca
      661 ccagcaccaa ttgccccagg ccccggcacc gaagaatcct tggctgccca aacggtctct
      721 ccccgcgctt gcgttccggt ttccctcata ggcggttgcg tctcccgcct gatctgccac
      781 ggacccgggg gcggcctccg ggtcagaatt ggcggtcctc cttgtgattg tccggggtag
      841 cgacagacgc tgaataggtt tacaaaaaag ggaaagtgat cacccgcggc ctgatcctgt
      901 gaatgtgggc aacgtcgaca gcatgccgct ttcttctctc ggaggcccaa ttgagccgag
      961 aataggtcgc ttcagcggtc tttcccgaag ggcagatagg ccggccgttt tacggctggt
     1021 tcgcggtcac tcgttcctct ctccttgttt ccccacattg agcccgtttc aacgggcttt
     1081 cccggatttc taagatggcc ccgcgctgac gctgggggtt ttgaaagagc cgcctggagg
     1141 gtgacgcaag cccgattccg ggctaccgtg ctcccggcat tgattcacct gccccgatgc
     1201 ggtgtaatgg cgggtctgtg ttcttggggt accagtgaga gacgacatca atggatctgt
     1261 ttacctacag gaatggcgag ttgtactgcg aggacctgcc ggtccgtcag attgtagatt
     1321 ccggcatcgg gaccccgacc tacatctact ccgcgacaac cttcagggag cattactccc
     1381 gcatggcgtc cgctttcgcc gagttggatc cgatcatctg ctactcaatc aaatgctgcc
     1441 agaacatcca catcgtccgc ctgctcgtcg agcagggcgc gggcatggac gtggtcagcg
     1501 gcggcgagct gtaccgggcc ctcaaggccg gtgcggatcc gtccaagatc gtttatgccg
     1561 gtacgggcaa gacggacaag gagatcaacg aggccatcga cgcaaggatc ggctggttca
     1621 acatcgagtc cgaggccgag ctggccaacc tgatccacat cgcttcgcag cggcagacga
     1681 cggtgcgcgc tgccctgcgg gtcaaccccg atgtcgatcc caagacgcac cgccacacca
     1741 ccaccggcaa aaaggagacc aagttcggcg tagatatcga gcgcgccaag agggcgttcg
     1801 aggaatacgg ccgcaacaag tcggtcaggc tctgcggcat ccatctgcac atcggctcgc
     1861 cggtcaacgc cgtccagccg tacgtcgagg ccatcgaaaa ggcattggac ctgatcctct
     1921 cgctgcggcg agacggcttc gagatcaaca cgcttgacat cggtggcgga tacggcgccg
     1981 actacagcgc cggcgaggcc ctgtccggca aggcctacgc ccagatgatt gtgccgctgc
     2041 ttcgcgaaca ggggctcaag atcatcctgg agcccggccg atccatcgcc gcaaacgccg
     2101 gcatcctggt cacccgcacg ttgttcatca aacgcagcgg cgaccgccag ttcgtcatcg
     2161 ttgacgccgg catgagcgac ctgatccggc ccgccctgta tgacgcctat catttcctgt
     2221 ggccggtcgc tccgggtccg cagtacgcac tggccgagcg tcgccggaac gtgaacatcc
     2281 ccgagaccgt taaggtcgac gtggtcggac cgatctgcga gaccggcgac ttcctggcca
     2341 gggaccgcga cctgccgccg attaaacgag gcgacctgct ggccgtcttt acggccgggg
     2401 cctacggttt caccatggcc agccactaca actcgcggcc caacgccgcc gaggtgctgg
     2461 tggaaggttc cacgttccgc accatccgcc ggcgcgagac ctacgatgat ctcatcgccg
     2521 ccgaagaaca ggtctgacca ctgcccgctg cgcccccagg ccgtggtcgc ttctccagcc
     2581 gcttccccgc gagccccgcc gctgcgccaa tggccagtcc gaccacgtgt gtcgcaccca
     2641 cgagcgccac ccccgctggt gccgaggtcg gcaggagcac ctgcccggtc aacgcttcga
     2701 tcactgattt ggccgcaacc agcgccagca ccccgatcca tgccatcgcc ttgcgccgcc
     2761 cgtcctggat cgccatcgtc gtcaggatcc acgccagcaa ggcgcaatcc acccccgaga
     2821 cgcctcgata agtggcgacg tcgtccgcca gcccgaacac cgcaagtccc accgccggcg
     2881 aagacagcgc caccacccaa gacagtcccc gtgatcgtcc ttccgcaatc cagcataatg
     2941 cggcgaacgc cataagattg gctgccaggt gtgcccagct atagtgaacg aagttgccgg
     3001 tcagaagccg ccaggtctgc ccggcagcga tatgaccgcg atcgaattcc agcatggact
     3061 ggaccgcagg cgacaggtgc accaacaagg agaacgcagc cacgccgatc cagaagaatc
     3121 gctcccagga gatggtaccc ggcagcaccg tgccgccggg tactcgcgtc acatggacat
     3181 tgaccgttgc agggtcggcc gctttcatgc cgcaccccgg cgacgacgac gccattccat
     3241 gaccgctaga aatgcgatgc cggctactcc cagcggaccg accgggccgc cgcctcgcgg
     3301 ccgcaggctg atgtcgcccg gccccgacgg gggcggaggc ggagcgccgg tcgagaccat
     3361 ctgctgtccg ccgagtggag cggtcggcgg agtcatggtc aacggctcca cgccgccgtc
     3421 gcgcggcccg aggtctgtca tcgccttgtt tcgtatcgct tcgaagtcgg cgatcagctt
     3481 ctgttgcgaa tcgcgatctc gttgcgagcg aagcacgttg cggcgatcga tcttccaccg
     3541 ctgatactcg gcgtcgttct ccaacaccag gaacgacgtg tacggagtca cgatggagta
     3601 agcctcgccc aatcgcacga tctcatttgc gaccgcggcg tgcgaacctg cgcggtctcc
     3661 ctccttcagc aaccgctgca ccttgtgcca cgcccacata cgctcgattt caggattcgc
     3721 gtcttcggcg tccgggaact tcagatcaac gctgctgacg atttcctgac cctccacggc
     3781 ggcacgcacc acggccttgc ccggcccctg cccgcgatag cgcccgtaca tccttgcggg
     3841 cgcgccgtga aacaggttgg gcaccaaggg cggctcaacg tcaaaggttt ccacaccctc
     3901 gatgctcacg cggatgttgg tcgccgccgg gcgcgtcagc ttccgccgga acgattgagc
     3961 ctgtcgctcg aagttgtcac caggcgacag gaatgaggcg agcccacccg aatcatcggc
     4021 catctgctcc agcagcggcc ggttgacctc gttgccgacc cccacgcaga acacacgggc
     4081 gttgcccggc cgcgatttga tcatctccag cagcgtgcgg gactcggcct gctcggtcat
     4141 tccgtcactg aaaatgacca cgttcagcgt acgggcggga tcggcgtact tgtaggcaac
     4201 ttccagagca ggcttcatca tcgtgccgcc cctcgcctcc tgtgaagcga gaaaggacga
     4261 tgctttcgcc gtactctctt cgccggtggt cgtcagggac ttgaagaggg aatgcggcga
     4321 cacgttgaag gtgatgatct cgaatcgatc gtccttgccc agcgaccgaa tgaacgcttc
     4381 caccgagttg cgggagagcg ctagcttgcc gtcgtcagcc atgcttcccg agctgtcgat
     4441 gatgaacacg tagtccatcc ccgaggtctc cttggccagt tcctccccgg cagtcaacgt
     4501 caggcagaag tatccgtcct caccgggtcg ccgagaggcg atcatgtcga tgccggtatg
     4561 cgggcgcgac acgtgacacg ccaccaccac gtcacgattc aggtcgcccg acttcgtctc
     4621 caggctcgcc tgaatctgcg tctcgccgtg ccgggcaacg gccatctggt ctccgtggct
     4681 ggggctttcc acctcgacaa tgggcacctg agacctggcc tgcagggcga acgagaatct
     4741 gcccgtcgtc ttcgaattga tgtccttgcg cgtctgcgtg gccagcgggt atacgtaggt
     4801 tgcccaatcg tggtcataat ccagttcctg gtagtagcag atctgcacct tctggtcagc
     4861 cttcggaccg atcgggaaga tccgcatctc aaaggtcttg aagtccttct gctccagcag
     4921 gcccgggtcg cgacgcacgc gtttgtagct gtcatagatc tcccgggccc tctgcttctc
     4981 caccacttcg ccgaccattt ccttgccgtt gatccacatg gagaaattgg acaccgaggc
     5041 gtctttcggc accgggaaag tgtagagcgc ctcaacctgg cgattctccg tattgcgaaa
     5101 tacctgggtc acctcggtca ccgcgatacc gttgttgatc gtcacctgca cgttgtgctc
     5161 gcggatttcc agtacgccgc cgagcccgcc atcggcaatc agcagccccg caccgctggc
     5221 ggcagtctgg atcatcagga tcatgcagcc ggccgcgatc gacccgccca tgcgtctgtg
     5281 tttcgaaccg ttcatctcat tctccttttg ctcttggact ctgtgcatgc gcgcaccttc
     5341 cacctcggcc cacacgctgc cgcgcgccga gccgcacttc gacccctgcc gccggttcat
     5401 cgctcggcga cagttgccca cgtcatcaag gaaaacccac tgaccgggcc gcgcgcatcc
     5461 cggaaaactc aggaaccctc catccgttgc ggcgggccct gctttgacag cagactcgcc
     5521 accatggact ccagttggtc caacgacgtc gtcgccgcat atcccttcgc ccacagcgat
     5581 tcactgggga agtcattcgg atgctttcgt ctgaccgcct tgcctgcgga ctgaaaacga
     5641 tgcacggtct tctccggcga gtgattcggc catgtctcga ccaccaggac gacatgatcc
     5701 ggctcaatct gacattcgat gattgaccat ccgtgctcga tggcctcgcg ttcaaaggcg
     5761 tgccgcatcc attccgcccg cggtccggcc aacagaggca acctgtgcca cgtaacccat
     5821 gccagtccgt aaaccacccg atgcaccacg tcttcgtcgg tcttgaaaac ctcgtggagc
     5881 ttgcgcttga cgtggctcat cgccgaaggc cggtgaacga gttgaatcga ccgggccttg
     5941 tcccgcgtgc gcgtgatgtg gcctttcttc tccagcgcct ccacatgccc tcgcacggca
     6001 ttgacattgg ccagacccag cgccgcgccg atctccctca gcgtgggtgg atagccatgt
     6061 cgctcgctaa agccgctgat gtaatcgagc acgttctgct gctggactgt caaaggcttg
     6121 ccgtccatac ggacctcatg aacactgtac gtatgtatag tatacaccac cgggccaccg
     6181 gatgcaaatc agatctacca aaaaatgcag gatcgcagat tgttcgcaat cgtatttatt
     6241 ataaggactt atgttttcca ggcgttggtc tatcgggtcg aggcctgctc ctcggcccag
     6301 cccgccggct cctcatcctg cttgaggtcc agcgtctggc ggccgacttg aatcaggaag
     6361 ctcagcaggg caatgagcca cgccagtgtc gattcggccc cttggttgtg attgggccct
     6421 tcgggcgtga gcccgtcgca acagccgccc gtacggaagt cgtacaaagg tgcatccaag
     6481 tcgttgcggc ccaggaacca ttcgaccgcc aggcgggcct tggctaacca tttctcctcg
     6541 cgagtcgcac gatatgcctc ggcacaggcc agcaccatct gcgaggcctc gatcggttgc
     6601 tgatcgaagg ttgccttctc gccgccccgt gagaaccatc cctggttgcc gacaaaggaa
     6661 aagtgcccgc gatcggagcc ctgcacgtcg cacaaccact gaaggctccg aagcccctga
     6721 tcacgcatct cgccatcggg aatccacgta cccgccagaa tcagggcgtg aaccagagtg
     6781 gcgttcgagt aggtcatggt cggctcgcac cactgccagt ccggcgagac gtttcgtcgg
     6841 aatagatcga acagcttctc cgccaatacc tggcggacgc gccgcacccg tgcgtcgccg
     6901 ctgaagtact ccaggtaggc gtgaatgccc aggagcgaat acgcccacgc ccgcgggtac
     6961 tcgaattgct cggccgaacc gatcacatcc tggaacaact gcaccgccat catccgcgaa
     7021 ttctcgttgg gcgcgtgagc ggccgtcatt cccaggcccc acagggcttt gccctgactg
     7081 tcttcgctgc ccaccgcgtc gagccactgt cgcgaatagg acatgaaatt acggaagcgg
     7141 ttggtcttct cgttataggc gtgagacaga tacgccaggt agatcccggc caggtcgtcg
     7201 acttcgtccg agtcgtagag ctgccgatga agcgcgcaga ccaccaaccc gcgcccgttg
     7261 tcgtcgagtg aatagccctg tcgccgatcc ggcgtcgagt acagacagtg ctgcaagaca
     7321 cccgtagggt cggtcatcgt ccgcaggtgg cgaacatcga cttcgggcag ctcctcggcc
     7381 cgagccacgc ggcgccgctt gtgggccagc aggcggggct gtcgtgcccg ttcgtggagc
     7441 agcgattgcc ccagcagcag atactcgcga gccacctgct tccaaaccat ctcccggcaa
     7501 taggtgtagg cccgcttgcg tatctggtgc cgctcggtgt cattctggaa caagtggatg
     7561 accgcgtccg ccagagcctt ggaatccttg aacggcacca gcacgcctcg cccgtcggcc
     7621 agcagttcct gggcatgcca gtagggcgtg gatacgactg ctttgcccgc gccgaccgcg
     7681 tacgccagcg tgccggaggt gatctgcgcc tcgtgcaggt aaggcgtcac gtaaacgtcc
     7741 gccgcgccga tgtactcgca cagttcctcc agttcaacga actggttgcg aaaccgaacg
     7801 tgctcctgca ggcccagatc cgccacgcga cgttgcagac tgctgcggta ggcctcgccg
     7861 aactggcgaa ccaggtgcgg atgcgtcgca cccaatacga tgtagagcac atcgggaaag
     7921 gctttcacga tctcgggcat cgcctcaatc atgtactcga tgcccttgtt cggagacagc
     7981 aacccgaacg tcaatatcac ccgccgcccc tcggccttga actggtcctt gtagtaattc
     8041 gggtccacga acggcacatc cggtatgccg tgcggtatca gcgccaccct ctcggcgggc
     8101 acgtggtaga cgtccagaag catctgtctg gcgcgatggc tcatcaccac cagc
//
LOCUS       DAOTPW010000257         8152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7880_length_8152_cov_5.195628, whole genome shotgun sequence.
ACCESSION   DAOTPW010000257 DAOTPW010000000
VERSION     DAOTPW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7880_length_8152_cov_5.195628"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..890)
                     /locus_tag="P5316_18295"
     CDS             complement(468..890)
                     /locus_tag="P5316_18295"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRR86965.1"
                     /translation="MMDRLGWRPALLGLAIMMPAVSGCVRRTMTINTAPQGARVILND
                     QEVGTSPVSIDFTWYGDYSVILEKEGYKTLQTNQFVVTPWYQTPGVDFFAEVLWPFPI
                     HDKREYTFELEPAGEPISRDDLLKNAEEFRDRAIFGED"
     gene            complement(887..1366)
                     /locus_tag="P5316_18300"
     CDS             complement(887..1366)
                     /locus_tag="P5316_18300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR86966.1"
                     /translation="MKIGILSDTHDRLPAIARALELFRQRQVEAVVHAGDLVAPFAAR
                     SMQSWSGPLHVIYGNNDGERDGLKRTLPQIRDGPLWIETGGKRILVHHYLDWCTPHDV
                     ERADVIITGHTHEVVNERQGDRLLLNPGECCGWLSGRCTVAILDTNSLSAEIVEVTP"
     gene            1528..5976
                     /locus_tag="P5316_18305"
     CDS             1528..5976
                     /locus_tag="P5316_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86967.1"
                     /translation="MSDEQSRPDAATDPASQPSVRQSVRKWLLRVFFSFVAVVVIAYG
                     TLPYWLPTGWLARQFENQLSTDLNRKVRIGAIRVGWRHGVSIENLTIAERSDQPDELL
                     ARIGRIACGFTPLTTLRTGRVDRLEIIEPEIWLAFDDEGRLISLQDLGARSTSRSGFP
                     TWEYRIRRASCHLRMPTLVQTFRIDDLTCRIDKPAGVLEVSGQTIIHRNDPVLSPGET
                     ATARLLIDGRVITPKLRKDMVLHGQARVEWEGLAITDLPLLVAMHFPIEQVDGSTDGT
                     LAFSVQPDLRIEYDLSLALKSVQILKSGTTEPAQVPDARFTGKGLWDPPTDRFALYEF
                     DYETQAIHLRGAGDADQPAFAMDPAGDTPLTIRLSGRVKDWVALGHEFPDVAEWTQSA
                     TARIEGAANLSLDFTSHQNDNRLIADVDGRELKLGIGPAASEYLCADPGMTKRLRLDI
                     THDHQTGKYTQRQLSLSIGSTTLESHGEMVVPAIEARDGWEWLAAVFPSLRWELTVRT
                     KDLAEISRLRPPERVPSELLRGRGPAEMTFSLIPQDGQSRLDVSIHVPAETTVNLGPR
                     RMVKPAGHPFSLTAGLGIPHQFAGRLENPVFSITYGQAGVQLNSEQAQAEVLATLEEG
                     RSVDFVDLRDERAGTTAVLNARWMLPLSVKGVEPLAGLFPDVTAAIGQGNLEGDASLS
                     IMGRFVSGAGDWLVRNELLLSARGLTARWRDLLDKPADEPLDIAFAHECQSIDGQCEQ
                     SLHALLRQPAGEISGSVIFSEGREEDGGDDFEVVTVGARIEDLERFVSLSPALRVFLS
                     DARISGGVRMQCQCLSSDGRSNGFVSLDADKAGFTTGRTAPLVKKPGTPAQMRLEWSS
                     EHLPGSTADRRFRLVDGSVQISRFTIEKLTGQVSIDPGDPSFHLLNWARHAVASKRLP
                     PRLRSAALQLAGRIEVDDPSWTKNPVWEQWRKWLNLTGNAGWRLETVLDENLFSLKGQ
                     LDGREIGLSCDLGQRWVPTLSKPPGTPADLRFHLAAARLPESQCIDIEARDIEFDLNG
                     NTLRMGGLLQVTADEDGAWQPRKLDVDCSVHLTDPVAVREALPGCRFEMLEGAAFGRA
                     VAGGTLQQLDVSLIEVGFDRLLVGIGDKPLGLDGQIVLDRQLLRFDQLACSWGQSKGC
                     IAGVFYAEDGERPRRARLGMVLEQFNQPELAALIIKLPLAPSTDSQPAGEATRVRRRI
                     VEFLHRLDLDLDIRVEEGTVVLPKDVQVEAEAAVNRVAIKDGYVNMSFGAIVDGGTVA
                     GSFTTDLKRTEPTFYLKYAANRIQPGPLVDRYLALTFPGMKATGPLTIIDETYQKLLP
                     AADEPNYEVGEGELMIEGGSVAGRAAPLWMTNIFPGLNFATFEFSYMHSWFKKFEDGR
                     IRHQMIFQGRFYNVYMVGECDAQGFMSYDVGIDFLANFDSRYWAESGQGRIPLFTKTG
                     RMMPDGSLANEEVVYVPRKFIMSLLVKNNPVVTAYHAVRKRVRGEQ"
     gene            6004..7584
                     /locus_tag="P5316_18310"
     CDS             6004..7584
                     /locus_tag="P5316_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRR86968.1"
                     /translation="MAFNDLQSFVAELERLDQLRRVKAEVDPVLEITEIADRVMKSPS
                     LEGPAGAPATDRTHGGLGGRALLFEKVRGSSMPVLINAFGSYARIRLALGCRDLDEIG
                     DRLRQMIKPEMPGTFLERLKKLPELLRLATFAPRSSKRGICQEVIHTDDASLLSLPIP
                     QCWPNDGDPASEPPQIHPPAEPIAEASGASGRTGRYITFGGVLTQDPDSGVLNMGMYR
                     VQVFGPKLAAVHWHVHHDGARIYRRWTARGEKMPVAIALGGESVLPYAATSPLPPGLS
                     ELFFAGFLNEQGIEMVAAKTIPMEVPANAEIVIEGYVDPQDRFIEGPFGDHTGFYSLS
                     GPYPVLRVTAITHRRDPVYPTTIVGYPPMEDYYLGKATERIFLPLVQLLVPEVIDYHL
                     PMFGAFHNCAFVKIRKEYPYQARRVIHSLWGAGQMSFSKFIVVVDEHVNVHDEQEVLF
                     CLGANVDPRRDTVIAEGPLDILDHAAPFEGAGSKMGIDATRKIPGEGPVRRWPARLEM
                     SPRIKEQVTRRWRELGLE"
     gene            complement(7839..>8152)
                     /locus_tag="P5316_18315"
     CDS             complement(7839..>8152)
                     /locus_tag="P5316_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86969.1"
                     /translation="YTVQSGYWQEVVRRELVREGYWRIVDPCEHQKKYYGTGGRECGE
                     VRFSMHLEKGDRDRGDGRHGVMMEKGPVPKPQMKAGMITEKVAKVADDGKLVKVGSVK
                     K"
BASE COUNT         1544 a         2563 c         2471 g         1574 t
ORIGIN      
        1 ctcacgacct cttgcatgcc atgcaagcgc tctcccaact gagctacggc cccggcggga
       61 ttatctctgt ctgcggtgtc cgcgttcaat aaagttctgc cccgtcggga tttcccgacc
      121 cggtcagact ctcttattac atcggcagag ggtcgatccg tcaagagggc tgtcggcgcg
      181 acccgacgcc ggggtccaga cactcctggc ggctctctcc ggccctccac cactcgggtg
      241 ggttcaaggg tccggcgggc cggggagaaa ggtaggtcaa acggccctcc accactcggg
      301 tgggtcaagc gaaacccacg gcagactcct atgagccggg ccaagcggcc tcccaccgct
      361 cgcgtgcgtc aaaaaggggc aggaccggag ggtgccgcac ctcaagggag ccggcctcga
      421 agcacagtcc gagacagcat cacggccggg ccggggactc ctcgcggtca atcttcccca
      481 aaaatggctc ggtcgcggaa ctcctcggcg tttttcaaga gatcgtcccg ggaaatcggc
      541 tcgccggccg gttcgagctc aaaggtgtat tctcgcttgt cgtggatggg aaaaggccag
      601 agcacttccg caaagaaatc gacacccgga gtctggtacc acggcgtcac cacgaactgg
      661 tttgtctgaa gcgtcttgta gccttctttt tcgagaatga cactgtagtc gccgtaccag
      721 gtgaaatcga tgctcaccgg cgaggtgccg acttcctgat cgttcaggat gacgcgggct
      781 ccctgtggtg cggtgttgat ggtcatggtc cggcgtacac agccggaaac ggccggcatc
      841 atgattgcca ggccaagcag ggccggccgc caaccgagtc ggtccatcat ggagtcacct
      901 cgacaatctc agcggagagg ctgttggtgt ccaggatcgc caccgtgcac cttccggaca
      961 gccatccgca gcactcccca gggttgagta acaacctgtc gccttgcctt tcattgacca
     1021 cctcatgggt gtgccccgtg atgatgacat cagcccgctc cacatcgtgg ggcgtacacc
     1081 aatcaaggta gtgatgcacg aggatacgct ttccccccgt ctcgatccag agaggcccgt
     1141 cccggatctg cggaagcgtc cgcttcaggc cgtcacgctc accgtcgttg ttcccgtaga
     1201 tcacgtgcag cggcccgctc caggactgca tgctgcgggc tgcaaaagga gccacgaggt
     1261 caccggcatg gactacagcc tcgacctgcc gctgccggaa gagttcgagt gcccgggcaa
     1321 tggcgggcag tcggtcatga gtatcggaca aaatgcctat cttcatcgcc ggtcacccgg
     1381 taccgaatga tacaacatca ccgccgtgaa gagtttatac tccgatcgcc ggcaccggac
     1441 aagcaccttt cgagctgtgg cacgacctcc gtcctgacat gaactcggtt gtggtcgggc
     1501 ggcgcgccaa ctacaatcgg ctgtctcatg agcgatgaac agtccaggcc ggacgcggcc
     1561 accgacccag cttctcaacc ctctgtccgg caatccgtcc ggaagtggct gttgcgcgtc
     1621 ttcttctcat ttgtcgccgt cgtggtcatc gcctatggga ctttgcctta ctggttgccc
     1681 acgggttggc tggccaggca attcgagaac cagctttcaa cggacctcaa ccgcaaggta
     1741 cgcatcggag cgatccgcgt cggatggcgg cacggagtct caattgagaa tctgaccatc
     1801 gcggaacgga gcgaccaacc tgatgagctg ctggcacgca tcggccgcat cgcctgcggt
     1861 ttcacgccgc ttaccactct cagaaccggc agagtcgatc gtctcgagat catcgaaccg
     1921 gagatctggc tggctttcga cgatgaaggc cgcctgatca gcctccaaga cctcggggca
     1981 cgctccacca gccgaagcgg tttccccacg tgggagtatc gtattcgacg cgcctcatgt
     2041 cacctccgga tgcctaccct ggtgcagact tttcgcatcg atgatttgac atgccggatc
     2101 gacaagcccg ccggagtcct cgaggtgtcc gggcagacaa tcattcatcg taatgaccct
     2161 gtgctctctc caggggaaac cgccacggca agacttctga tcgacggcag agtgatcacg
     2221 ccgaagctcc gcaaagacat ggtgcttcac ggacaagccc gtgtcgagtg ggaaggcctg
     2281 gcgattacgg acctgccgct gttggtggcc atgcactttc cgatagagca agtggacggc
     2341 tccacagacg gcacacttgc cttctccgtg caacccgacc tgcgcattga gtacgacctc
     2401 tcgctcgcgc tcaaaagcgt gcagatcctg aagagcggga ccacggagcc tgcccaggtg
     2461 ccggatgctc gcttcacggg caaagggttg tgggaccccc ctaccgaccg ttttgccttg
     2521 tacgaattcg actacgaaac gcaggctatt catcttcgcg gcgcaggtga tgctgatcag
     2581 ccggcattcg caatggatcc ggccggggat acgcccttga ccatccgcct gtctggccgg
     2641 gtgaaagact gggttgcgct cggtcacgag ttcccggatg tggccgagtg gacacaatcc
     2701 gcgacggctc ggatcgaggg ggctgccaat ctctcgctcg atttcaccag ccaccagaat
     2761 gacaaccgcc tgatagccga cgtcgatggg cgagaactga agcttggcat aggaccggcg
     2821 gcttcggagt atctgtgcgc cgatccgggc atgactaagc ggcttcgcct ggatattacc
     2881 cacgatcacc agacaggcaa gtacacgcag agacaattgt cgttgtcgat cggcagcacg
     2941 acccttgagt ctcatggaga aatggtcgtt cccgccatcg aggctcgtga cggatgggaa
     3001 tggttggcgg ccgtgtttcc cagccttcgg tgggaactga cggtccgcac caaggacttg
     3061 gccgagatct cacgcctgcg gccgccggag cgtgtcccga gcgagctcct ccggggacgc
     3121 ggaccggcgg agatgacatt ctcgctcatt ccgcaggatg ggcagtcgcg tcttgacgta
     3181 tcgattcacg tgccggccga gacgacggtg aatctcggac ctcgtcggat ggtcaagccc
     3241 gccggtcacc ccttctcgct gaccgccgga ctcgggattc cgcatcagtt cgcgggcagg
     3301 ctggaaaatc ccgtattcag tatcacatac gggcaggcag gtgttcaact gaattcagaa
     3361 caggcccagg ccgaagttct tgcgaccctg gaggaagggc gttcggttga tttcgtggat
     3421 ctccgcgacg agagagcggg taccacggcg gttctcaatg cgaggtggat gctgccgctg
     3481 agcgtgaagg gcgtggaacc gctggccggc ttgtttccgg atgtgaccgc cgcgatcggc
     3541 caaggaaatc tcgaagggga cgcgtctctc tcgatcatgg gtcgattcgt cagcggggcc
     3601 ggcgactggc tggtacgtaa tgagctgctg ctgagcgccc gcggtctgac ggcgcgatgg
     3661 cgggatctgc tcgacaagcc ggccgatgag ccgctggata ttgcgtttgc ccatgagtgc
     3721 cagtccatcg acggacaatg cgagcaatcg ctacacgcat tgctgcggca gccggcggga
     3781 gaaatcagcg gctcagtgat cttttcggag ggccgggagg aggacggagg tgacgacttc
     3841 gaagtcgtga ccgtgggtgc gcgtattgaa gacctcgagc ggttcgtctc cttgtcaccg
     3901 gcgctccgcg tctttctgag cgacgcccgg atctccggcg gcgtccggat gcagtgtcag
     3961 tgtctgtcat cagacggccg ttccaacggg tttgtctctc ttgatgcaga caaggccgga
     4021 ttcacgaccg gccgcacggc cccgctggtc aagaaacccg ggacgccggc acaaatgcgc
     4081 ctggagtgga gtagtgagca tctccccgga tcgaccgcag accggcggtt ccgtttggtc
     4141 gatggttccg tccaaataag tcgtttcacg atcgagaaac tgaccgggca ggtctccatt
     4201 gatccggggg atccgtcctt tcatctcctc aactgggctc ggcatgctgt cgcaagcaaa
     4261 cgattacccc cccgactcag gtcggcagct ttgcagcttg ccggcagaat cgaggtcgac
     4321 gatccgtcgt ggacgaagaa ccctgtttgg gagcaatggc gcaagtggct caacctcacg
     4381 ggtaatgccg gctggcgtct tgagaccgtg ttggatgaga acctcttttc cttgaaggga
     4441 cagctggatg gacgggaaat cggtctgtca tgcgatcttg gccaacggtg ggttccaacg
     4501 ctttcaaagc ctcctggaac accggcagac ctccggttcc atctcgcggc ggcccggctt
     4561 cccgaatctc agtgcatcga catcgaggca agagacatcg agttcgatct caacggcaac
     4621 acgctgagaa tgggcgggtt gttgcaggta accgccgacg aagacggtgc gtggcaaccg
     4681 cgtaaactcg acgtcgactg ctctgttcat ctaacggacc cggtcgctgt ccgggaggca
     4741 ctgccaggtt gccgttttga aatgctggag ggcgccgctt tcggaagggc tgttgccggc
     4801 ggcacgctgc aacagcttga tgtgtcgctt atcgaagtcg gcttcgaccg attgctcgtc
     4861 ggcatcggcg acaaacccct cggactggat gggcagatcg tccttgatcg gcaattgctc
     4921 cgcttcgacc aactggcctg ctcatggggc caatcgaaag ggtgcatcgc gggggtgttc
     4981 tacgccgagg acggcgagcg gcccaggcgc gcccgcctcg gaatggtgct cgaacagttc
     5041 aaccagcccg aattggcggc gctgatcata aagcttccgc ttgcgccatc aaccgactct
     5101 cagcccgcgg gcgaggcaac gagggtgagg cggcggatcg tcgagttcct gcatcgcctc
     5161 gacttggatt tggatatccg cgtcgaagaa ggcacggtcg ttctgcctaa ggatgtacag
     5221 gtcgaggctg aagcggcggt caaccgcgtc gcaatcaagg acggttatgt caacatgagc
     5281 ttcggggcga tcgtggacgg cggaaccgta gcgggttctt tcacgaccga tctgaagcga
     5341 acagagccga cgttctacct caaatacgcc gccaaccgga tccagccggg cccattggtc
     5401 gatcgctatc tcgctctgac cttccccggc atgaaagcca ccggaccgct gacgatcatc
     5461 gacgagacct atcagaagct cctgccggcc gcggacgagc ccaactatga agtcggcgag
     5521 ggcgaattga tgatcgaggg cggctcggtc gccgggcggg ctgcgcccct ttggatgacc
     5581 aacatctttc ccgggctcaa tttcgctacc ttcgagttct cgtacatgca ctcatggttc
     5641 aagaagttcg aggacgggcg catccgtcac cagatgatct ttcagggcag gttttacaat
     5701 gtctacatgg tcggcgagtg cgacgcccag gggttcatgt cgtatgacgt gggcattgat
     5761 ttcctggcca atttcgacag caggtattgg gccgaatcgg gccagggacg catcccgctg
     5821 ttcaccaaga cgggcaggat gatgcccgac gggtcattgg ccaacgaaga ggtggtctac
     5881 gtgccgcgca agttcatcat gagcctgctg gtcaagaaca accccgtggt cacggcatat
     5941 cacgccgttc gcaagcgggt gcgcggcgaa cagtgaggtc cttgcggggc aggagcggcg
     6001 atcatggcgt tcaacgatct tcagtctttc gtagccgagc tcgagcggct cgatcagctc
     6061 cgacgggtca aggccgaggt cgatcctgtc ctcgagatca ccgaaatcgc ggaccgggtg
     6121 atgaagtcgc ccagcctcga aggcccggcc ggggcccccg ccacggatcg cacccacggc
     6181 ggcctgggcg ggcgggcact gctgttcgag aaggtccgcg gctcgtcgat gccggtgctg
     6241 atcaacgcct tcggcagcta cgcccgtatc aggttggcac tcggctgccg cgacctggac
     6301 gagatcggcg accggcttcg tcagatgatc aagcccgaga tgcccggcac gttcctggaa
     6361 agacttaaga agctgccgga gttgctgcgg ctggcaacct tcgcgcccag gtcctcaaaa
     6421 cgcgggattt gccaggaggt gatccacacg gacgatgcaa gcctgttgag tcttccgatt
     6481 ccgcaatgct ggcccaatga tggcgacccc gcctccgagc cgccgcagat ccatccgccg
     6541 gccgagccaa tcgcggaggc atcaggggct tccggtcgaa cgggccgata catcaccttt
     6601 ggcggcgtgc tcacacagga tccggacagc ggcgtgctca acatgggcat gtaccgcgtg
     6661 caggtgttcg gtcccaaact ggccgccgtt cattggcacg tgcaccacga cggagcccgc
     6721 atctaccgcc gctggaccgc ccgcggcgag aagatgcccg tcgcaatcgc tctcggcggc
     6781 gagtcggtct tgccctacgc ggcgaccagc ccgctgccgc ccggactcag cgagctgttc
     6841 tttgcaggtt tcttaaatga acagggcatc gagatggtcg cggcgaagac tatccccatg
     6901 gaagtgcccg ccaatgccga gatcgtcatc gagggctacg tcgacccgca ggaccgtttc
     6961 atcgagggtc cgttcggtga ccacaccggc ttctactcac tctcgggccc ctacccagtg
     7021 ctccgggtca cggccatcac ccatcggcga gaccccgtct acccgacgac catcgtcggc
     7081 tacccgccga tggaggacta ctacctcggc aaggccaccg agcgcatctt cctgccgctg
     7141 gtgcagttgc tcgtgcctga ggtgatcgat taccacctgc cgatgttcgg ggcgtttcac
     7201 aactgcgcgt tcgtcaagat ccgcaaggaa tatccctatc aggcccgtcg cgtgatccac
     7261 agcctgtggg gggccggaca gatgtccttc agcaagttca ttgtcgtggt cgacgagcac
     7321 gtgaacgtgc acgatgaaca ggaagtgctg ttttgcctcg gtgccaacgt cgacccgcgt
     7381 cgcgacacgg tgatcgctga aggaccgctg gacatcctcg accatgccgc cccgttcgaa
     7441 ggtgccggca gcaagatggg catcgacgca acgcgcaaga tccccggcga gggccccgtc
     7501 cgccgctggc ccgcccgcct ggagatgtca ccgcggatca aggaacaggt cactcgccgc
     7561 tggcgggaat tagggttgga atgagcggcg gcgggccctc tgcgctcgcg gaccaacggc
     7621 agcgtccgca agcccatttt tcgcatgcgc tcgccattgt cttcgagaat caggaacatt
     7681 ccaacatcca gaaggcccaa gcagcttctc cccacgccga ctgccgtctg gtgatctctt
     7741 cccgatcccc ccatgaaaac gcccaccggc aaccgggggg ctgcccgatt gccggtggtg
     7801 tgtttccctc gcacgtcgct ctcgctcgcc aatgaccctc acttcttcac cgaccccacc
     7861 tttaccagct tgccgtcgtc ggcgaccttc gccaccttct cagtgatcat ccctgccttc
     7921 atctggggct tggggaccgg gcctttttcc atcatcacac catgccggcc atcaccccgg
     7981 tcacgatcac ctttctccag atgcatgctg aacctcacct cgccacattc acgaccgccg
     8041 gtcccgtagt atttcttctg gtgctcgcac ggatccacga ttctccagta gccctcccga
     8101 accagctcgc gacggaccac ttcctgccag tagccgctct gaacggtata gg
//
LOCUS       DAOTPW010000258         8147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7889_length_8147_cov_4.790040, whole genome shotgun sequence.
ACCESSION   DAOTPW010000258 DAOTPW010000000
VERSION     DAOTPW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_8147_cov_4.790040"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="P5316_18320"
     CDS             <1..552
                     /locus_tag="P5316_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR86970.1"
                     /translation="AQYGDARATGLKGESPATSPEARTALVEGKKLKKAKIRLKREDV
                     DPGRIAIHGRSLGGGVAAQIAARRKPAALILESTFTSVASYAQKYCVPKLLVRHPFRT
                     DLVLPELDVPVLVFHGTRDDIVPVKHGRRLAKLARNAVYVEYDCGHNDAPGPGNEADY
                     WGRIEAFLEEAGITRPTAAQAVD"
     gene            692..1591
                     /locus_tag="P5316_18325"
     CDS             692..1591
                     /locus_tag="P5316_18325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR86971.1"
                     /translation="MSTIRRREFLGKAAGVLGSALAPAAFGEPPIAAPASRPTSAPAK
                     LKAGDVITLGRTGIKASRLAIGTGTESGAEQRRLGIEGLVKLLRHGFDQGVRWWDVAD
                     SYKTHPHVKAALKEMSRDEVTITSKTSAKDAGGIKADIERFRREMGTDYIDIVLLHCM
                     MDGDWPEKLKGARDALSEAKQKGLVRAVGCSCHTFDALQAAADSDWVDVDLARINPFA
                     AVMDINRKDDVPQVLQILQRMHERGTAVYGMKILGAGTFKGNMIERSIRFILQQKCVS
                     AFVIGFSKAEDIDDIVRRIERVQ"
     gene            complement(1613..2737)
                     /gene="pdxA"
                     /locus_tag="P5316_18330"
     CDS             complement(1613..2737)
                     /gene="pdxA"
                     /locus_tag="P5316_18330"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRR86972.1"
                     /translation="MSEAPPTNSVSNESNPKPLVGITMGDPAGIGAEVIVKALCDPEV
                     RRLGRFIIFGLDESLEFAADQAEINPFWFRVPHEAATRIESGVIVADFDDCPVFNPHT
                     HCPTAEAGHASMRFLDAAIKSAREEVIEAIVTAPICKESWHLAGYRYPGHTEKLGHAF
                     KTERLTMMFVGGKLRVALASIHQALFDLRNSFTIGRVFQPIDLMAGALRTWFGIEYPR
                     IAVCGLNPHAGENGLFGDEEKRVIEPAIVMAREAGWFVEGPYPADTVFWHASRGRFDG
                     VVAMYHDQGLIPVKLLAFESACQTTLGLPIIRTSVDHGTAFDIAGQNIANPESMKSAI
                     RLACHAALCKRNQPAVPQPVPAPWEELSGVDPGDDDETEN"
     gene            complement(2745..3386)
                     /locus_tag="P5316_18335"
     CDS             complement(2745..3386)
                     /locus_tag="P5316_18335"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRR86973.1"
                     /translation="MFTGIVQAKGTVRSVRPTGNGVRLVLDAPGLPRPISNGASVCVS
                     GVCLTVTRCDAQTIEFDVVAETLSRSTLGGLKPGDRVNLERSLRAADRLDGHIVQGHV
                     DGTAIVSGISSDHTATFEATEDLRTYIIPKGSIAIDGVSLTIADVSGRHFSVALIPST
                     LAETTLGNLRVGDRVNIETDILARTVVTTLRRWRDGSCGHGITMDLLQRQGYA"
     gene            3672..7373
                     /locus_tag="P5316_18340"
     CDS             3672..7373
                     /locus_tag="P5316_18340"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRR86974.1"
                     /translation="MTAVIGLASSAGLCFGEVSPSYVLADADGNALGAAEAGIEDIEN
                     RLAVAVERAGAPDPQSGESLLPAGTGLDRFEWVDDRAHVDLTFPAETAPDTIGPLRLE
                     DIYEVLANAVDPFRMSRGLVIRARRGPTAEYLPLEAYTPTVVLPPEQDVREESGIVIE
                     ERSRAFAEASTGEQVVEAWIGGPVEHAGTQPVGALTGVTVFCSAGHGWTAGSSSWYLQ
                     RSLLLDMVEDYGNIDQLNYFVQYLYNAGATVVPFRPVGYQAVEVVLDNDDPGVTYSGT
                     WTNSTATTEYYENGTTVSGVPYRWATAAVTESATARYTPNIPATDFYPVYCWTRDGTD
                     RVRQTYRIAHSGGVSSVTVDHRLVGKGWIWLGDYYMLAGASSYVEITNASPDTGVVIA
                     DAIRFGNGMGDIVRPGPGTISGYPREEECSRYWAQSEAGNNAVGLPQVWETSGDDGSD
                     NVGTAARWSAVMNRQNVNNDRWRRIYIEFHTNAAGCSPPPCSAKGTICLVTNTGATTY
                     QTEYATILGDKVEADMRLIDDDLFEYLWGLRSNPYTSAYGAISTSNNNDEFDATIIEV
                     AFHDNVEDTANLLNAKVRNAVARSTLQGMIMFLNSLPGSTVPLVFLPDPPENVRAVHN
                     GQGSIVVSWNAPPSGGANGGAAAGYRVYRSSNGYGFGQGLDVGNVLTTTLTDVPAGTT
                     TYLRVTAYNAGGESLPSRTMAVRREPDASTQLLIVDGYNRVSRHQNVVQVIPAGPMQR
                     PIARKVNSFDYVVQHATAMSDSGVAFDYAENSAVAGGAVSLNNYRAVIWILGQESTAD
                     KTFDSNEQMIVTAYLNAGGNLFVTGSDIGYELDGQAAGRPFYENMLRANYEADNAGTY
                     SVLGAGGIMADVGPFDFNPNNGAAYVVKTPDRISPQSGAAAILSYGGGNGTAGIQYDS
                     GVYRVVMFGFPFEAISTSSARSAVMQRVLNYILPPMGCPYGPDAITGFEGYADGTHVM
                     FQDPRYSGSTLAHLAATPNSAVVTSEVQAYSGGKTCRVDWAWLDASPTRWLRLTTHNV
                     ANVPNPTIDLRRPVRVRLRLDSGSFRISLGVRETGADVPIGENGGTSGTIEWVGATSV
                     VPGGGPVGTLVSAAPGVWQTITFAPYVGYIQPMTGDGVLNAAHDKGVLEHLAFTITDT
                     VGPITVYLDSIEQPCPPKADFDLDGDVDLIDFGLLQACISGASKPQTAAECQVAKLDG
                     DGDVDQDDCSLFLMCLSGPGVPAVPECLN"
     gene            7864..7936
                     /locus_tag="P5316_18345"
     tRNA            7864..7936
                     /locus_tag="P5316_18345"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7897..7899,aa:Lys,seq:ttt)
BASE COUNT         1510 a         2472 c         2649 g         1516 t
ORIGIN      
        1 gcgcagtatg gggacgcccg cgcgacgggg ctgaaggggg aatcgccggc gacctcgccc
       61 gaggcgcgga cggcgctggt cgaaggcaag aaactgaaga aggcgaagat ccggctcaag
      121 cgcgaggacg tggatcccgg caggatcgcg attcacggcc ggtcgctggg cggcggggtg
      181 gcggcgcaga tcgctgccag acgcaagccc gcggccctga tcctcgaatc cacgttcacc
      241 agcgtggcct cgtatgcgca aaagtactgc gtgcccaagt tgttggtcag gcatcctttc
      301 agaacggatc tggtgctgcc cgagctggat gtgcccgtcc tggtatttca cggcacacgc
      361 gacgatattg tgcctgttaa gcacgggcgt cggttggcga aactggctcg caacgcggtg
      421 tacgtcgagt acgattgcgg gcacaacgat gctcccggcc ccggcaatga ggcggactat
      481 tgggggagaa tcgaagcgtt tctcgaagaa gcggggatca ctcggccgac tgccgcccaa
      541 gcggtcgatt gaggctcgat cacgtggagt atcgaccggt ttttccggtt ccgctttgac
      601 cggcgatcat tggttgccgt gcgcgccgcc gcggactagc ataagaagcg tgtcggcctg
      661 gatgtgacac ggttgtttcg gaagggggca tatgagtacc attcgtcgcc gtgagttcct
      721 gggcaaagct gcgggggtgt tggggtctgc tcttgcgccc gccgccttcg gtgaaccgcc
      781 gatagccgct ccggcgtccc gcccgacgag cgcgccggca aaactgaaag ctggcgacgt
      841 gatcaccctt ggccgaacgg gcataaaagc ttctcgtctc gccatcggca ccggcaccga
      901 gtccggggcg gagcagcgac gcctgggcat cgaagggctg gtcaagttgc tccgtcacgg
      961 attcgaccag ggcgttcgct ggtgggacgt ggcggattca tacaagaccc atccccatgt
     1021 caaggcggcc ctgaaggaaa tgtcccgcga cgaggtcacg attacctcca agacctccgc
     1081 gaaggacgcc ggaggcatca aggcagacat cgagcgtttt cgacgggaga tgggcaccga
     1141 ctacatcgat atcgtccttc ttcactgcat gatggacggc gattggcccg agaagctcaa
     1201 aggggcgcgg gatgccctca gcgaggcgaa acaaaaaggg ctcgtgcgag ccgtcggctg
     1261 ttcgtgccac acgtttgacg ccctgcaagc cgccgccgac agcgactggg tggacgtcga
     1321 tctggcgagg atcaacccgt tcgctgccgt catggacatc aacaggaaag atgacgtgcc
     1381 gcaggtgttg cagattctgc agcgcatgca cgaacgaggc accgcggttt acggaatgaa
     1441 gattctgggg gcgggcacgt tcaagggcaa tatgatcgag cggtcgatcc ggttcatctt
     1501 gcagcagaag tgcgtgagtg cttttgtgat cgggttcagc aaggccgagg acatcgacga
     1561 catcgtccgt cgtattgaac gggttcaata gcgggtgatg cgtcgcagca ggtcagttct
     1621 cggtctcgtc gtcgtcgcct gggtcgactc ctgacagttc ttcccatggg gcgggcaccg
     1681 gttgcgggac ggcgggctgg ttcctcttgc acaatgccgc gtgacacgcc aatcggatcg
     1741 cggacttcat actctcgggg ttcgcgatgt tctggcccgc gatgtcgaac gccgttccat
     1801 ggtccacgct cgtgcggatg atcggcaggc cgagcgttgt ctgacatgca ctctcgaatg
     1861 ccagcagttt gacgggtatg agcccctgat cgtgatacat ggccaccacg ccgtcgaacc
     1921 ggccgcggga agcgtgccag aagaccgtgt cggccgggta ggggccctca acgaaccatc
     1981 cggcctcgcg ggccatgacg atcgccggct cgatgacgcg cttctcctcg tccccgaaca
     2041 gcccgttctc cccggcgtga ggattcagcc cacacaccgc gattcgcgga tactcgatgc
     2101 cgaaccaggt ccgcaaggcg ccggccatga ggtcgatggg ctggaacacg cggccgatcg
     2161 taaaggaatt acgcagatca aacaatgcct gatgaatact ggccaaggct acgcgcagct
     2221 tccccccgac gaacatcatg gtaagacgct ccgtcttgaa ggcatggccc agcttctcgg
     2281 tatggccggg gtaacgatat ccggccaggt gccagctttc cttgcagatg ggagcggtca
     2341 cgatggcttc gatcacctcc tcgcgtgccg acttgatggc cgcgtcaaga aaacgcatcg
     2401 aggcgtgccc cgcctcggcg gtgggacagt gggtgtgcgg gttgaagacg gggcaatcgt
     2461 cgaagtcggc cacgatgacg ccgctctcga tgcgggtggc cgcctcatga ggcactcgga
     2521 accagaaggg gttgatctca gcctggtcgg cggcgaattc aagcgattcg tccaggccga
     2581 aaatgatgaa gcgcccaagg cggcgaacct cggggtcgca cagggccttg acgatgacct
     2641 ccgcgccgat tccggccggg tcgcccatgg tgatacccac caacggcttg gggttgcttt
     2701 cattggacac ggagttggtc ggaggcgcct cgctcatgat gaggtcacgc gtatccttgc
     2761 cgctggagca gatccatcgt gatcccgtgc ccgcaggacc cgtcacgcca tcgccgaaga
     2821 gtggtcacga ccgtacgggc caggatatcc gtttcgatat tgacacggtc gccgacgcgc
     2881 aggttgccca gcgtcgtttc ggccagagtg ctcgggatga gtgccacgct gaagtgtcgt
     2941 ccggagacgt ccgcgatagt caacgagacg ccgtcgatgg cgatcgagcc cttagggatg
     3001 atatacgttc tgaggtcctc ggtggcttcg aacgtggcgg tgtgatcgct gctgatacca
     3061 gaaacgatgg cggtgccgtc aacatgcccc tgcactatgt gtccatcaag gcggtcagca
     3121 gcccgcaggc tgcgctcaag gttgacgcgg tcgcccggtt tcagcccgcc gagcgtgctg
     3181 cgcgaaaggg tctctgcgac cacgtcgaat tcgatggtct gggcgtcgca ccgggtgaca
     3241 gtcaggcaga ccccgctgac acagacgctg gcgccgttgg atatcggacg cggcaaaccg
     3301 ggagcgtcca gcaccagccg gactccgtta ccggtgggac gaaccgaacg tacggttccc
     3361 ttggcttgta cgatgcccgt gaacatggcc tttcacctgt tgacgactgg atgcggcctg
     3421 ccacttgcct gcctcatgct agctccgtgc gcattgtagt gtcaaggttg gagctgactg
     3481 ccgaggctga cgatgacact gcccgaggaa cagtgtcttt ggaaggtgcc gatggccatg
     3541 gacattgacg ttctgttttc agccatctac ttcatatggt ataatgcctg cggagatcgg
     3601 ggatcgtcgt ctcggggagg cggagggatg atttcatgca agtgcggcat ggctgttggt
     3661 gtcagtgttg tgtgacagcg gttatcggtc tggcatcaag cgccgggttg tgcttcggtg
     3721 aggtttcgcc ttcttacgta cttgccgatg ccgacgggaa tgccctggga gccgcggagg
     3781 ccggaatcga ggacatcgaa aaccggcttg cggtcgctgt cgagcgcgcg ggcgcgccgg
     3841 atccacaaag cggcgaatcg ttactcccgg ccggaaccgg tctggatagg ttcgaatggg
     3901 tcgatgaccg ggctcacgtt gacttgacgt tcccggccga gacggccccg gacacgattg
     3961 ggccgcttcg gcttgaggac atttacgagg ttctggccaa cgcggtcgat ccgtttcgga
     4021 tgagccgcgg cctggttatt cgagcccgcc gagggccgac agccgaatac cttcctctag
     4081 aggcgtatac ccccaccgtt gtgctgcccc ccgagcagga tgttcgcgag gagtccggaa
     4141 tcgtcatcga ggagcggtcc cgcgcgttcg ccgaggcttc caccggcgag caggtcgttg
     4201 aggcctggat cggaggcccg gttgagcatg ccggaaccca accggtcggc gcactgaccg
     4261 gcgtgaccgt gttctgttcg gccggccacg gctggaccgc cggatcgtcg tcctggtatc
     4321 tccagcgttc cctgttgctc gacatggtcg aggactacgg caacatcgat cagctcaact
     4381 atttcgtcca gtatctctac aacgccgggg ccaccgtcgt gcccttccgg cccgtcggat
     4441 accaggccgt cgaggttgtt ctggacaacg atgacccggg cgtgacctac agcggaacgt
     4501 ggaccaatag cactgcgaca actgagtatt acgagaacgg caccaccgtc agcggtgtac
     4561 cctaccgctg ggccactgcc gcggtcaccg aatcggccac ggctcgctat acgcccaaca
     4621 tacctgcgac cgatttctac ccggtctatt gctggacacg cgatggcacc gaccgcgtgc
     4681 gccagaccta ccgcatcgcg cacagcggcg gggtcagctc ggttacggtg gaccatcgcc
     4741 tggttggcaa gggctggatc tggctgggcg actactatat gctggctgga gccagcagtt
     4801 acgtcgagat caccaatgcg tcgccggaca cgggcgtggt catcgccgat gccatccgtt
     4861 tcggcaacgg catgggtgac atcgtccggc ctggcccggg caccatcagc ggctaccctc
     4921 gcgaggagga gtgttcccgg tattgggctc agagcgaggc gggcaacaac gcggtgggcc
     4981 tgccccaggt gtgggagaca agcggagacg acggaagcga caacgtcggg acggcggccc
     5041 gctggtccgc cgttatgaac cgccaaaacg ttaacaacga ccgctggcgg cgcatttaca
     5101 tcgagttcca caccaacgcc gccgggtgca gccctccgcc gtgctcggcc aaggggacca
     5161 tttgcctggt caccaacacc ggtgccacca cttaccagac tgagtacgcc accattctcg
     5221 gcgacaaggt cgaggccgac atgaggctga tcgacgacga cctgttcgag tatctctggg
     5281 ggttgcgatc gaatccgtac acttcagcct atggcgcgat ctccactagc aacaacaacg
     5341 acgagttcga cgcgaccatc atcgaggtgg ccttccatga caacgtcgag gacaccgcca
     5401 acctcctcaa cgccaaggtc cgcaatgccg ttgcccgctc gactttgcag ggcatgatca
     5461 tgttcctcaa ctcactgccc ggcagcacgg tgccgttggt cttcttgccc gatcccccgg
     5521 agaacgtccg ggcggtacac aacgggcagg ggagtattgt cgtgtcatgg aacgctccgc
     5581 caagcggtgg ggccaacggc ggggcggccg ccggatatcg cgtgtatcgc tcgagcaacg
     5641 gctacgggtt tggacaggga ttggatgtgg gtaacgtgct caccacgacg ttgaccgacg
     5701 ttcctgcggg tacaacgact tacctgcgag tcaccgctta caacgccggg ggcgagtcgc
     5761 tgccctcacg gacaatggcg gtgcgtcggg agccggatgc gtctacgcag cttttgatcg
     5821 tggacgggta caatcgcgtg agtcggcatc agaacgtcgt tcaggtgata ccggccggac
     5881 cgatgcagcg accgatcgcc aggaaagtca actcgtttga ctacgtcgtc cagcacgcga
     5941 cggcgatgtc ggacagcggc gtcgcgtttg actatgccga gaacagcgcc gtcgccggcg
     6001 gggccgtttc cctcaataac taccgggcgg tgatttggat cctgggccag gagtcgaccg
     6061 cggacaagac gttcgattcg aacgagcaga tgatcgtgac agcctacctg aatgcgggcg
     6121 ggaacctctt cgtgaccggg tccgacatcg gatacgagct ggacggccaa gcagccggcc
     6181 ggccgttcta tgagaacatg ctcagagcga actacgaagc cgacaacgcc ggcacctaca
     6241 gcgtgctggg agcgggcggc atcatggccg atgtcgggcc gtttgatttc aatccgaaca
     6301 acggcgccgc ctatgtggtc aagacgccgg accgaatctc gccgcagtcc ggtgcggcgg
     6361 ccattctgtc ctacggcggc ggtaatggga cggcaggtat ccaatacgat tcgggcgtat
     6421 accgagtggt gatgttcgga ttcccctttg aggcgatcag tacgtcttcc gcccggtcgg
     6481 ccgtcatgca acgcgtgctg aactatatcc tgccgcctat gggttgcccg tatgggccgg
     6541 atgcgatcac gggtttcgaa ggatatgctg acgggacaca cgtcatgttc caggatcctc
     6601 gatattccgg atccacactg gcccaccttg ccgcgactcc gaactcggcc gtcgtgacca
     6661 gcgaggtgca ggcatacagc gggggcaaga catgcagagt ggattgggcc tggctcgacg
     6721 cgagcccgac ccggtggctc cgtctcacga cgcacaacgt tgccaatgtg cccaacccta
     6781 ccatcgatct gcggcgcccg gtgagggtgc gactgagact cgacagcggg tcgttccgaa
     6841 tcagtttggg cgttcgcgag acgggggccg acgtgccgat cggcgagaac ggcggcacca
     6901 gcgggaccat cgagtgggtg ggggccacgt cggtcgtgcc cggcggcggt ccggtgggga
     6961 cgctggtgtc ggccgcgccg ggtgtctggc agaccatcac cttcgccccc tacgtaggtt
     7021 acattcagcc catgaccggc gacggtgttc tgaacgctgc gcatgataag ggtgtactcg
     7081 aacacctggc atttacgatc accgacacgg tcgggccgat cacggtgtac ctggatagca
     7141 tcgagcagcc ctgcccgccg aaggcggact tcgaccttga tggggacgtc gacctgatcg
     7201 acttcggcct tctccaggca tgtatcagcg gcgccagtaa accacagact gccgccgagt
     7261 gtcaggtggc gaaactcgac ggcgatggcg atgtcgacca ggatgactgc agcctgttcc
     7321 tgatgtgtct cagcgggccg ggcgttcccg cggttccgga atgcctgaat tgaggggtcg
     7381 ggtgggggcc gcgctcccgg accttcgtcc ctgaaggatt cgcaaccgca aacgtatccg
     7441 tcgcccagcc ttggcgaaag aggaagcgag cagcccccgc cgcatgggct tgcgaccgcc
     7501 tcgcgcgttg gcttggcgca agtccctctg ttttcgcaag tcaagcagct ccctgcgacc
     7561 gcctcgcggg ttggcttggc cttgaccgac ttacggggat tgggcaatgg tccgtcgctt
     7621 acgtttcggt ttctgtaaca ggcagccagg ttaaccgccc caaacggcac agcctccccc
     7681 atcgctgcaa caaaccgaag ttggcgagcc gcccggaaac ggtgtagaat gaccttgctt
     7741 aggccctgcg gccagcggcc cagagcctgc cgataccaaa ggacgccggt tgtcatgccc
     7801 acaagcggcc tctagccgga ccgctccacg catgtgcggt aagtccttat actgcaagat
     7861 cttgagggcg tagctcagta ggtagagcaa cggcctttta agccgtgggt ccagggttcg
     7921 agtccctgcg ccctcaatcc tttcccctcg gtgccagtcg gtgcaagagc gtgcaagtgg
     7981 gtgtccaagt tgtccatctt cctgatggcg taaggtcagc caccggaaac ccgggcggtg
     8041 ggcctcaccc gacggtaatg gccgctacgg gccttgccgc gagtaccgcc gccgcatccc
     8101 cagcccccga aggccacgag caggggcgtg gcgagtctgt tctcggt
//
LOCUS       DAOTPW010000259         8100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7948_length_8100_cov_5.385457, whole genome shotgun sequence.
ACCESSION   DAOTPW010000259 DAOTPW010000000
VERSION     DAOTPW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7948_length_8100_cov_5.385457"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(525..2096)
                     /locus_tag="P5316_18350"
     CDS             complement(525..2096)
                     /locus_tag="P5316_18350"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRR86975.1"
                     /translation="MNLFAAQSDREPILFRPDGVGASLGFYLPATMLSRFMGLIRGVV
                     LTWLISESQYGLLQIAMLSISILHPLLGVGFNEAMTRYVPQYETRGALRAFLARALAG
                     AMLISLVVCGLAWLGAGPLGRFLFEIMPEAGGLPAGFDVTLIGHLAVAATFCLVLYFL
                     MLGILRGLRMFRAISAIETVYQVGFTVVAVVIALFGWKSAQAMLICYAASVLGTVLLF
                     SPCLVRVIGSAADQPEPLSESGRALLGQMLRFSIWAALAALMWQTLQYYPVWYLHKVC
                     GREGEVTGIFAAMRLLAQAVLVIALAVVTVVQTSVTKTWETQGRELADRLLLLGFKAT
                     ALVLLGLSAVLAVSARLLVMIYPSSYSIGQAAITPSLLNYVLWSYLLFQTIHFTLIEK
                     TRHVFVPWCLGLLCTVALSKWLVQPGLPDEAAILAAAWAGVLGITPAMLACLVLLKLE
                     KRPFDFGTWLLLAAASVLALPLPLMVPVMGLVLLLAAGTTAILNHEEKRQIRDFLATG
                     HGTARQILSQLVGWM"
     gene            complement(2128..2805)
                     /locus_tag="P5316_18355"
     CDS             complement(2128..2805)
                     /locus_tag="P5316_18355"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HRR86976.1"
                     /translation="MREKGWDIAIAVVRNGRPPVPGAIVPGVAPAALPSLRSLWSALF
                     PASDRSGGYCLMVTAAERGEGVSQIATGLALAGPAICPDRRIAVVDFNFKRPMVGRLF
                     RIPAVQGVADVLAGRLPIEEAPIQTQEMAIDVLPAGCANGSVLCPFPWPVAGQLIDHL
                     RAYYDYILLDAPAVNVDDLTAELGCLADGVLLVIRAGTTHREAVMEARFRLDRAGARL
                     VGAVLND"
     gene            complement(2814..4475)
                     /locus_tag="P5316_18360"
     CDS             complement(2814..4475)
                     /locus_tag="P5316_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86977.1"
                     /translation="MPKSATTRFLLRIGRALMVRPIGTGALFLATLGACMAVCFLAEW
                     SDRASITLAVRTLGAAPASSEAAAGDDRSAFSTVYLDLLRSDTVLCRTLLVVSYPDSP
                     AAAEWRKARHEWEGAHSPEAWAALSKALGQLDAEAERLRRDEVTGRMILAGLAGLSER
                     VDVMPCGSQGLLDVVKMSVSWPVRRGDVRLVADCLSQMACDRLKEIQMRAAQEASDFI
                     RLRRESLRPDQLAPAENAMRRFVEEELTSPADLVHLQQLSRSAEEADLPVFIKRVRQE
                     LLALDTQRAEARNLRRLLLEALPASLWDGARRRSDEGELIGPDMSRIDEKHLPDHDPI
                     LADVAQVIPREALDRNVVLSRLKTREAELLQDLNRLRSEFNPAYMGIADKRAELARTR
                     RQILTQVIGEAAQLQVSIAGMAARQTELQQRLEAAERQLQRLTGKLGRYQELTQELDA
                     SRKRYFQAWLDETQAMKDQWERLPTAVLHVVDISADYAGRRALLASPWKAALTGCIVG
                     FVLALAYAVAADFLDRTLRWPDEVERHVKLSVVGRIRKSGNRIVA"
     gene            complement(4575..5384)
                     /locus_tag="P5316_18365"
     CDS             complement(4575..5384)
                     /locus_tag="P5316_18365"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR86978.1"
                     /translation="MNSFKQSMCGRSAQTAFYSGVLLVLLTGCTQLGPRERQSLIEAK
                     GLYDQNRFSEAINRLNPVIKDFGQAAEIGEAYYLRGLCRSKLNDSKGAAADFEQAIAS
                     SRNDAVIIRCKISLAAVSFQMGQWSKAADLYVEVVPRLEDKPPADQVLFYAGTALRRA
                     GRWREATQYFARILHRFPSSPIAADARRMAGWQHEYFAIQLGAYQDAANAERAVQAYR
                     TRNLDFVQMENQPSGGRVLWVVMAGRHRTYQDAVAALNRIRTIVPDANIIP"
     gene            5588..7084
                     /locus_tag="P5316_18370"
     CDS             5588..7084
                     /locus_tag="P5316_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRR86979.1"
                     /translation="MVIESLLDAAGRFPAKTAVIDPFRRLSYAQLTTLAKVIRRIVLK
                     ETACRRVGILLPGSSAGMSTLLGVIWAGRTAIPLNFLLQARELTSIIEDADIDLVIGT
                     RYFESILATLPIRTLYIEQLGLPRRYLWEKLRPTPEPPPVSPDDTAAIVYTSGSTGRP
                     KGVCLSHANFENNVQAAIRHLQLDPENHLLGVLPPFHVFGLTILNLLPVMLRATVTFI
                     PRFSAQAVYETIAANPDITLLLAVPSMYAAIGRLKSIDASRCRGIKIAVSGGEPLPRK
                     IYDLMLERTGIRLMEGYGMTETSPVISCDLPSAHRVGSVGLPLPGVEVQIRNENGCSL
                     PANQTGELYVRGPSVMKGYYNRPEETKAVIDKDGWLRTGDIVQIADDGHITITGRSKD
                     IIIVGGENIYPREIEAVLEQHPAVQEAAVVGQTDEMRGEVVVAFVILNGCSQVTGAEL
                     RAFCRDHLAGFKVPREIFVRDSLPRTPSGKILKRQLMATLAGRRNDDA"
     gene            7487..7930
                     /locus_tag="P5316_18375"
     CDS             7487..7930
                     /locus_tag="P5316_18375"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRR86980.1"
                     /translation="MELVTQFSVFMVNKPGVLAQVTQQLAQAKINVLAMTVMDSSEHG
                     VLRLVTADVERARVALRKLNLPTMETEVLMVEMPNRPGALADICMRFANAHINISYAY
                     CTTGGAGGKARAVFKLADTRKGLKVLSKPEPKRREQNHRRLMLRR"
BASE COUNT         1677 a         2742 c         2310 g         1371 t
ORIGIN      
        1 gccgggtgcc atgcccacgg ctttgcgtgg gcatgcgcct ccaaatgaac cgctcggccc
       61 ggaatccttt ccgggccgca ctcctcgcgg aatcccttcc gcattcggca ttcatgacga
      121 cgccaatcct ccacccgaag gccgggtgcc atgcccacgg ctttgcgtgg gcatgcgcct
      181 ccaaatgaac cgctcggccc ggaatccttt ccgggccgca ctcctcgcgg aatcccttcc
      241 gcattcggca ttcatgacga cgccaatcct ccacccgaag gccgggtgcc atgcccacgg
      301 ctttgcgtgg gcatgcgcct ttgaatcctt acggccggac atctgtgggg ttcgcgcgca
      361 tattgccggt ttacgcgcgc ttggcctgcg gcccgcaaaa agacggtccg ccttaggatg
      421 agcccccact tgctgctgag cctttcgacg cgctcagggc gaacccagga acggggcacc
      481 cgcacatagt ccgcctcaat tcatccttca tcctttcttc agcctcacat ccatccaacg
      541 agctgtgaga gaatctgtct tgccgtgccg tggccggtgg cgaggaaatc gcggatctga
      601 cgtttctcct catggttgag aatcgcggtc gtcccggcgg ccaacaggag aacaagaccc
      661 atgaccggca ccatcagcgg gagcggcagg gccaggacac tggcggccgc cagcagcaac
      721 caggtgccga aatcgaacgg ccgcttctcc agcttgagca ataccaggca ggcaagcatc
      781 gcgggcgtga tgcccaggac accggcccac gccgccgcca ggatggccgc ctcgtcgggc
      841 aagccgggct gaaccagcca cttgctcagc gcgacggtac atagaagccc caggcaccat
      901 ggaacgaaga cgtgtcgcgt cttctcgatc aaggtgaaat gaatagtctg aaacagcagg
      961 tacgaccaca gaacgtagtt cagcagcgac ggcgtgatcg cggcctggcc gatcgagtac
     1021 gacgaaggat aaatcatcac cagcaatcgg gccgagacgg ccagcactgc ggaaagcccc
     1081 agaaggacca gggccgtggc cttgaatccc agcagcaaga gccggtcggc cagttcccgc
     1141 ccctgggtct cccaagtctt ggtgaccgac gtctgaacca ccgtcaccac cgccagagca
     1201 atgaccagga ccgcctgggc cagcagtctc atggccgcga agatgcccgt cacctcgccc
     1261 tcgcggccgc agaccttgtg caagtaccag accggatagt actgcaaggt ctgccacatg
     1321 agcgccgcca acgccgccca gatgctgaaa cgaagcattt gcccaagcag agcccggccg
     1381 gattccgaca gcggctcggg ctgatcggcg gccgaaccga tcacccgcac caggcaaggg
     1441 gagaacaaca acaccgtccc cagcacgctg gccgcgtaac agatcaacat cgcctgagcg
     1501 ctcttccatc cgaacaaggc gatcaccacc gccaccaccg tgaatccgac ctgatagacc
     1561 gtctcgatcg cactgatcgc gcggaacatg cgcaggcctc gcaggatgcc gagcatcagg
     1621 aagtagagca ccaggcaaaa cgttgctgcg acggccaagt ggccgatcag tgtgacatcg
     1681 aaaccggccg gcaatccgcc ggcctccggc atgatttcaa acaggaagcg gcccagcgga
     1741 ccggccccca accacgccag gccgcaaaca acgaggctga tcagcatcgc cccggccagc
     1801 gccctggcca gaaacgcccg caatgccccg cgagtctcgt attggggcac gtaacgagtc
     1861 atcgcctcat tgaagccgac acccagcaac ggatgcagaa tcgagatgga caacatcgcg
     1921 atctgcaaca ggccgtattg gctctcgctg atcagccagg ttagcacaac gccccggatc
     1981 aaacccatga agcgcgaaag catcgtggcc ggcaggtaga agcccagcga agccccaacg
     2041 ccgtcgggtc tgaaaagaat cggttcgcga tcggattgag ccgcaaacaa gttcatcgcc
     2101 gatcgtcggg ctccgtcaaa gaaagcatca gtcgttaaga accgcaccga caagccgcgc
     2161 acccgcccgg tcgagtctga acctggcttc cataacagct tcgcgatgcg ttgtccccgc
     2221 cctgatcacc aggagcactc catcggccag gcaacccagt tcggcagtga ggtcatcgac
     2281 gttcacggca ggggcatcca ggagaatgta atcgtaatac gcccgcagat gatcgatcag
     2341 ttgcccggcg acgggccaag ggaaaggaca aaggacgctg ccgttagcac atccggccgg
     2401 cagtacgtcg atcgccattt cctgcgtctg aatcggcgct tcctcgatcg gcaatctgcc
     2461 ggccagcaca tcggccacgc cctgcaccgc aggaatccga aagagcctgc cgaccatcgg
     2521 acgcttgaaa ttgaaatcga ctacggcgat ccgtcgatcc gggcagattg ccgggcctgc
     2581 cagggccagt cccgtggcga tctgcgaaac gccctcgccc cgctcggcgg ccgttaccat
     2641 gagacaatag cctcccgacc ggtcagacgc cgggaacagc gcggaccaga gagaacgcaa
     2701 gctcggtaat gccgcggggg caacgcccgg tacgatcgct ccgggcacgg gcggtcggcc
     2761 gttgcgaacc accgcgatcg cgatatccca gcctttttcc ctcatgccca acctcaggca
     2821 acgatccggt tgcctgattt cctgatacgt cccaccaccg agagcttcac gtgtcgctcc
     2881 acctcgtccg gccacctcag cgtgcggtcc aggaaatcag ccgcaaccgc atacgcaagg
     2941 gccaacacaa acccgacaat gcaacccgtc aacgccgctt tccacgggct ggccagcagc
     3001 gcccgccgac ccgcataatc agccgatata tccaccacgt gcaggacggc cgtcggcagc
     3061 cgctcccact gatccttcat cgcctgggtc tcgtcgagcc atgcctggaa atagcgtttc
     3121 cgggacgcat cgagttcctg ggtcagctct tgatagcggc ccagcttccc cgtcagacgc
     3181 tggagctgac gctcggcggc ctcgaggcgc tgctgcagct ccgtctgacg agcggccatg
     3241 ccggcgatcg agacctgcaa ttgcgccgcc tcgccgatca cctgcgtcag gatctgacgc
     3301 cgagtccggg ccagctcggc ccgcttgtcg gcgatcccca tgtacgcggg gttgaattcg
     3361 ctgcgaagcc ggttcagatc ctgcagcagt tccgcttcgc gggtcttcag tcggctcagg
     3421 accacgttgc gatccaaagc ctctcgcgga atcacctgcg cgacatcggc caatatcggg
     3481 tcgtggtctg gcaaatgctt ctcgtcgatt ctcgacatgt cgggaccaat gagctccccc
     3541 tcgtcacttc gccgccgagc gccgtcccac aaggaggccg gcagcgcttc gagcaagagc
     3601 cggcgcaggt tccgggcctc cgcccgttgc gtatcgagcg cgagcagttc ctgtcgcact
     3661 cgcttgatga agaccggaag atcagcctct tcggcagagc ggctcagttg ctggagatga
     3721 accagatcgg ccggcgacgt taattcctcc tctacgaacc ggcgcatggc attctcggcg
     3781 ggagccagtt gatccggcct caggctctcg cgccggaggc gaatgaaatc cgaagcttcc
     3841 tgagcggccc gcatctggat ttccttcaac cgatcgcacg ccatttgcga caaacagtcc
     3901 gctaccagcc tcacgtctcc gcgccttacc ggccaggata ccgacatctt cacgacgtca
     3961 agcaatcctt gcgacccgca aggcatcacg tcgactcttt cggacaatcc ggcaaggcct
     4021 gccaggatca tccggccggt gacctcgtcg cgacggaggc gttcggcttc ggcatccagt
     4081 tgacccaggg ccttggaaag ggctgcccat gcctcgggcg aatgggcacc ttcccactcg
     4141 tggcgtgcct ttcgccattc ggccgccgcc ggggaatcgg gataacttac cacgagcaaa
     4201 gtgcggcaaa ggacggtgtc cgaacgaagc aggtcgagat agaccgttga gaaggcggag
     4261 cggtcgtcac cagcggccgc ctcactcgat gccggggctg cccccagcgt tctgacggcc
     4321 agcgtgatcg atgcccggtc ggaccactcg gccaggaagc agacggccat gcacgcgccc
     4381 aacgtcgcga ggaaaagggc acccgtcccg atcggccgta ccatcaatgc acgaccgatt
     4441 cgcagcaaga accgggttgt cgcggacttg ggcatagccc cataacccct cgggcaggcc
     4501 gccgtcggaa gattcacgat tcggcagggc gctgaccgcc ggacgcctgc cccctgccct
     4561 aaccagttta accgctaggg aatgatattg gcatcgggca cgatcgttct gatccgattg
     4621 agcgctgcca cggcgtcctg gtaggtgcga tgccggccgg ccatcacaac ccaaagcacc
     4681 cggccgcccg acggctgatt ctccatctgg acaaaatcca gattacgcgt gcgatacgcc
     4741 tggaccgccc gttcagcatt cgccgcgtcc tgataggccc cgagctgaat tgcgaagtac
     4801 tcgtgctgcc agccggccat ccggcgagcg tctgcggcaa tcgggctgct gggaaaccga
     4861 tgcagaatcc gggcaaagta ctgggttgct tcccgccatc gcccggcccg ccgaagggcc
     4921 gtacccgcgt agaaaaggac ctggtcggcc ggcggcttgt cttccaacct cggcaccact
     4981 tcgacataga gatccgcggc cttggaccat tgccccatct ggaaggaaac agccgccagc
     5041 gagatcttgc agcggatgat gaccgcgtcg tttctgctcg atgcgatggc ctgctcgaaa
     5101 tccgccgccg cccccttgga gtcattcagt ttgctgcggc acagtcctct caggtagtac
     5161 gcctcaccaa tctcggcggc ctgaccaaag tctttgatca ccggattcag cctgttgatc
     5221 gcctccgaga accgattctg gtcgtacagc cccttggcct cgatcaggct ctgacgttcc
     5281 cgcggcccga gttgggtgca gccggtcaag agaaccagca gaactccaga ataaaaggcc
     5341 gtttgagccg accgcccgca catgctctgt ttgaagctgt tcatcgtgac ctcatccgca
     5401 tacggcgatg atcatggcga ttccgcccac gcattctaac cacattcaag ctttcacggc
     5461 agcgctcggc cgctgcccga tcaaaacagc tcaccgacgt tctccatcgt ccgactgtgg
     5521 acgcaggccg cacgacatga tagcatgcct gcgtcttcac agctcgccaa cccggagctc
     5581 gtgcgtcatg gtcattgaat cactcctgga tgccgccggt cggtttcccg ccaagacggc
     5641 agtcatcgac cccttccgtc gcttgagtta cgcccaactg acaaccttgg caaaggtgat
     5701 tcgccgtatc gttctgaagg aaaccgcctg ccggcgggtc ggcatcctgc tgccgggcag
     5761 ttccgcggga atgagcacgc tgctgggcgt gatctgggcc ggacggaccg ccatacctct
     5821 gaatttcctc cttcaggccc gcgaactgac ctcgatcatc gaggatgcgg acatcgacct
     5881 tgttatcggc acccgctact tcgagtcgat cctggcaacg ctccccatcc gtaccctcta
     5941 tatcgaacaa ttgggcctgc ctcgccgcta tctttgggaa aagctcagac caacaccgga
     6001 acccccgccg gtttcaccgg acgacaccgc ggcgatcgtc tacacctcgg gcagcaccgg
     6061 acggcccaaa ggcgtctgtc tgagccacgc caacttcgaa aacaatgtac aagcggcgat
     6121 acgtcatctt caactcgacc ccgaaaacca tctgttgggc gtgctcccgc ccttccacgt
     6181 gttcggcctg acgatcctca atctgctgcc cgtcatgctt agggccaccg tcacattcat
     6241 cccccggttc tccgcccagg cggtctacga gacgattgcg gcaaaccccg acatcactct
     6301 gttgctcgcc gtgccaagca tgtacgccgc catcggtcga ctgaagtcaa tcgacgcctc
     6361 cagatgccgc ggcatcaaga ttgccgtcag cggcggggaa cccctccctc gcaagattta
     6421 tgacttgatg ctcgaaagaa caggaatccg gctgatggag ggctatggca tgaccgagac
     6481 ttcgccggtg atttcctgcg atcttccctc tgcgcatcgt gtgggctccg tcggtctgcc
     6541 gctgccgggc gtcgaggtcc agatccgcaa cgagaacggt tgctccttgc cggcgaacca
     6601 gacaggcgaa ctctacgtcc gcggtccttc cgtgatgaaa gggtactaca accggccgga
     6661 agagacgaaa gcggtcatcg acaaggacgg atggctgcga accggggaca tcgttcagat
     6721 cgccgacgac gggcacatca ccatcaccgg ccgcagcaag gacataatca tcgtcggggg
     6781 cgaaaacatc tacccccgcg aaatagaagc ggtcctcgaa caacatcccg ccgtccagga
     6841 agccgcggtc gtcggtcaga ccgacgagat gcggggcgag gtggtcgtcg cctttgtcat
     6901 cctgaacggg tgttcccagg ttacgggggc cgagttgaga gcgttctgcc gcgaccacct
     6961 cgccggcttc aaggtgccgc gggagatctt tgtccgggac agcttgcccc gcaccccgag
     7021 cggaaaaatc ctcaaacgcc aactgatggc gaccttggcc ggccgccgca acgacgacgc
     7081 gtagaatcgg tcgccacaac tcgctgcaca cgtgcccgaa ccgacgagtc cttcgtagac
     7141 cgttttttca ttggcctttc ggcggcaccc ggttataata gatctgtgag catctcaaga
     7201 cgttccaaaa ccgcaagcat ctgcgccctg acaacagaga ggtccgcttg cgctccgctc
     7261 atcaccgatt cagccgccgt ctccatgaac aaccccaccc tgccggcaaa gctgccggtc
     7321 agccggggaa ctacggtgcg tgcaaggccg cgacagcata tcacaaccgg cacgtcgggc
     7381 ggtggtggcc catcggccgg ccggccggcg acgagtacgg cggtaacgag cagtcgaggg
     7441 cgaccgatgt gtgtaacatg ccccacatga cgacaggagg tgtgcgatgg aactggtgac
     7501 acagttctcc gtgttcatgg ttaacaagcc gggcgttctg gcccaggtga cacaacagct
     7561 tgctcaggcc aagatcaacg tcctggcaat gaccgtcatg gactccagcg agcacggcgt
     7621 cttgcggctc gtgaccgccg acgttgagag agcaagagtg gccctccgca agctgaatct
     7681 gccgacgatg gagaccgaag tgctgatggt cgagatgccc aaccgacccg gcgccctggc
     7741 cgacatctgc atgcggtttg ccaacgcaca catcaatatc agctacgcat actgcacgac
     7801 cggaggcgcg ggcggcaagg cacgagcggt cttcaagctg gcggacaccc gcaagggtct
     7861 caaggtgctt tcgaaacccg agcccaaacg ccgtgaacaa aaccaccgtc ggctgatgct
     7921 ccgaagatag gttcaacgac acccgacaag ctgcaaccgc aaataagaac gtccaccgag
     7981 atgacaggat aagccggcac cagcgacgcc ggctgggttg gaactccgct caccgcacgc
     8041 cgtatggcgg catcagaaac aatgtatgcc accgtcccat aatgccggcc ccgctgagcg
//
LOCUS       DAOTPW010000260         8093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_7957_length_8093_cov_5.028241, whole genome shotgun sequence.
ACCESSION   DAOTPW010000260 DAOTPW010000000
VERSION     DAOTPW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7957_length_8093_cov_5.028241"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..1205
                     /locus_tag="P5316_18380"
     CDS             444..1205
                     /locus_tag="P5316_18380"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR86981.1"
                     /translation="MFHLALMSREGAYVGGMLMCLGLTLAGCSTGGEWKVLADGNSLD
                     GWKGFNSADCGKWMTAESVALDPAEQGKKFAITPGKGILVNGADGRTCDLITAEDHGD
                     CELHLEFTVAKNSNSGVYLLGRYEIQILDSFGKTEWTYSDCGGIYPRWINNENTDGHK
                     PMVNAAKPAGEWQSFDVVFRTARFDANGKKTANARFVRVYLNKVLIHKDVECSGPTRG
                     AIGGDLGPDVAAGPLRLQGDHGPVAYRNIKMRPLR"
     gene            1509..1898
                     /locus_tag="P5316_18385"
     CDS             1509..1898
                     /locus_tag="P5316_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86982.1"
                     /translation="MEKQITPAEGKAPAPRIFYDAPAELSMADKRRIFKGMVVSELEA
                     GFLRYSRREALLKYAAKLKIPEFEACLLIAEAQFHAGDIEPIEFQSAATLEAITQPES
                     WSIPLRLAVALAIATVIDLILVHLLFG"
     gene            complement(1976..3268)
                     /gene="hemA"
                     /locus_tag="P5316_18390"
     CDS             complement(1976..3268)
                     /gene="hemA"
                     /locus_tag="P5316_18390"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRR86983.1"
                     /translation="MRTFVVGCNHRSAPVEKREKIAFDEAAVPAALQRFKQRFPEAEA
                     VLLSTCNRVEFYVSRPMQGRPRIDEMIAFIGEFHGIEMHDFADTLYIHEDVQAVRHLL
                     RVASSLDSMVLGESQIVGQTRDAFKTARMAGTVGRNLGPLFQHALAVAKTIHATTGIS
                     IGRLSVGSTAVDLARQIFSNFGDKTVMMVGAGGMGKMTLTHLIETRPQRVWVTNRTHA
                     RAVEVAERLSRRHPLTVQPIPFADWIDRLAEADIVITCTGSREPILTAEAFDQIPARR
                     AYRPILLIDIALPRDIDAAVGRHDCVYLYNIDDLQAVTEAHLAQRKEALAKCHEIIEA
                     AVLELADRQSREDVGPLIAALEKHFREVGRQELERILPKLEKASAHDRELIEQMLHRV
                     TGKLLHGPITRLTGDSAAGTGRVYAEMLRALFDLKTEE"
     gene            complement(3265..4083)
                     /gene="ccsA"
                     /locus_tag="P5316_18395"
     CDS             complement(3265..4083)
                     /gene="ccsA"
                     /locus_tag="P5316_18395"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRR86984.1"
                     /translation="MTQLLTCILGGMGFAIGAICSYLRLRHHVRWAPAALRVAVTLAL
                     AANVGYLAGRFRSMGVVETFRYSFDDAVLLATLLGLMGISTHLARTLRGVDGFLFIIA
                     TALQCAALAIVNQPEMRITGRAWFVSHSIAFAISGTLFIAGGVAGVAYLLVNRMLRRK
                     QPTTLVGNVASLESLERFGRWTPIIGFPFFTYGILTGLCGVSHRPDLQADDWYLDPVF
                     ISSLLAWAVFGYLCYGSLYKPQIRGKRAAVLSTYGLGLIVVAYLFRVFFSLMHA"
     gene            4221..5606
                     /locus_tag="P5316_18400"
     CDS             4221..5606
                     /locus_tag="P5316_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86985.1"
                     /translation="MRGARFVGRVGLLVLTIATSPAVVRADADSAPGGRAAEAQSDDI
                     RRVVALLGHPVPSQRQAARRQLVQWGLRVTDELRRVAEGPNLEAAFQARDLLEALDSA
                     FFQGGQISVEADRAEVDWDEPFALTVTVRNPTRGPLKVTWAAPTTAPASGPADEAAQV
                     AALLDVADFLVVRGPDGQEVDVRIDPIERDPVVRAVVERRASGTQPTHLVGPGGEARL
                     RIPEFNRGWARYPTLSRGKYQISIRHQPAWKDQNWVEEGLGLIESPPIEVRVRSGAPA
                     SIRHANRPMRLQLKQTGGELLIQMRNLWDRPQWVNLDFGDDMEVQARLTWQAHRLTLF
                     GDEDGVELEPRVKAVERREHLLRLAPDEATEVGRVSMGDFREALVKAGGRRSGRYAVT
                     VRYQFLSEVENLRAALAARKSKIEVPTHLYTGVIFCREDVNVQDEAKIIGRQDAAPSK
                     PSEPWRMAKPE"
     gene            complement(5704..6408)
                     /locus_tag="P5316_18405"
     CDS             complement(5704..6408)
                     /locus_tag="P5316_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86986.1"
                     /translation="MRSAGMAVAIAAIAAAGCEPYRVHVDCRLTSDNSLWHRLDGPAF
                     AIHGPTPQIESTLEFEDYSAMLARALRQQRPVLRRVPIGQTANLQMTMAYTVLYRGQA
                     IETYPEFRPVYGFGPFGAPLFDCVGTEVRTVDLGYHHMLSLSAWIADQGLPAGRKVLW
                     ECRADLIAYDSNLKQTMPAMLLAATSLYGQSTGTTVTIKYGKDDDRLDTLRREPIRIT
                     APPPAQSLPASRPTNP"
     gene            complement(6433..7722)
                     /locus_tag="P5316_18410"
     CDS             complement(6433..7722)
                     /locus_tag="P5316_18410"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRR86987.1"
                     /translation="MNKTATLIIDGKSYELPVVIGTENEVGIDISRLRSMTGCITLDD
                     SYANTGSCLSSITFIDGEKGILRYRGYPIEELAEKSSFVEVAYLLIFGELPTSRQLAE
                     FRSLLMEHEFIHEDLRNHFEGFPPSAHPMAILSAMMNAMGCYQPDLLQPEDDTHFIDA
                     AARIMSKVRTIAAASYKKSIGHPMMYPRPDLPYVPNFLHMMFSKPNAPYEPSPEIIAA
                     LNLLLIMHADHEQNCSTSTVRMVGSSLANLYASVAAGICALWGRLHGGANQAVVQMLL
                     GIQQSGEDLKSYIAKVKEKKSNIRLMGFGHRVYKNFDPRSRIIKSACDRVLAQLGIRD
                     PLLDIAKQLEEIALSDPYFLERKLYPNVDFYSGIILRAIGIPVDMFPVMFAIGRMPGW
                     IAHWREVHLSPNPRIYRPRQIYVGPPLRHYVPMEQRQ"
BASE COUNT         1446 a         2240 c         2827 g         1580 t
ORIGIN      
        1 cgttggccgt cgcgtcaagg gttccggcgt cccaggtata gtcgaacgtg ccatcgtgcg
       61 tcaggttccc gtcgtcgtaa ataggtgact gtccctattt atccctattt atccgtgcgt
      121 caggttcccg tcgtcgtaaa taggtgactg tccctattta tccctcaata aacgtacctg
      181 tcccgttttc cgcgtgcgtt ttccgcgtgt gtcccgtttt ccgcgtgccc gttttccgcg
      241 tcgggaaagg tcgctgaagc ggcctgtttc cggctcgcgt ctgctgctga gccagagaac
      301 cacgacgagg gccgcctgtt tggtcacgaa cctcggtctt gccggaagac gattggcatc
      361 tcgcacgccc gctggtttcg tggtatcctg ccgtgcctgt tggttgtcgc ggttttgact
      421 gtgtttccag aaggagtgta gacatgtttc atctggcgtt gatgagtcgg gagggtgcgt
      481 atgtgggcgg catgctgatg tgcctggggc tgacgttggc cgggtgttcg acgggcggcg
      541 agtggaaggt gctggccgac gggaacagcc tggacggttg gaagggcttc aactccgccg
      601 attgcggaaa gtggatgacc gccgagtcgg tggcacttga tccggcagag cagggcaaga
      661 agtttgcgat cacgccgggc aagggcattc tggtcaacgg ggcggacggg cggacctgtg
      721 acctgatcac cgcggaggac cacggcgact gcgagttgca cctggagttc acggtggcca
      781 agaactccaa ctcgggggtc tacctccttg gtcgctacga gattcagatc ctcgacagct
      841 tcggcaagac cgagtggacc tacagcgact gcggcggcat ctacccgcga tggatcaaca
      901 atgaaaacac cgacggccac aagccgatgg tcaacgcagc caagccggcg ggcgagtggc
      961 agagtttcga cgtggttttc aggaccgcgc gttttgacgc taacggcaag aagacggcca
     1021 acgccaggtt tgtgcgggtc tacctcaaca aggtcctgat ccataaggac gtagagtgca
     1081 gcggtccgac gcgtggggcg atcgggggcg atctgggccc ggatgtcgcg gccggtccgc
     1141 tgcggttgca gggcgatcac gggccggtgg cctacaggaa catcaagatg cggccgctgc
     1201 ggtaggtggg ttggagcagg cagctagtcg ggccaccggg ggcaaggagg ggcaagatcg
     1261 ccatagcggc cgaggcctgt cattgtcctg atgtttgccg tcctctggta acgaccggcg
     1321 gcttggcggg cacatgggtt gcgcagcccg ttgttgggcg gtccgataaa catcgcgcgt
     1381 cgggcagatg tcaaaactct ccggatttgt tgaagaaacc gcgagttccg ggttattgcc
     1441 cgtagatcgg tttgtcgcgg atcggtagaa tccatcgaca gcgaggagtt gtccggagga
     1501 gaagtcccat ggagaagcag attaccccgg cggagggcaa ggcgcccgct ccgcgcattt
     1561 tctacgacgc ccctgctgaa ttgtcgatgg ccgacaaacg gcggatcttc aagggcatgg
     1621 tcgtatcgga actggaagcg ggttttctcc gctattcgcg ccgcgaggcg ctgctcaagt
     1681 acgcggccaa gctgaagatt cccgagtttg aggcctgcct gctgatcgcc gaggcgcagt
     1741 ttcacgccgg cgacatagag ccgatcgagt ttcagagtgc cgcgacattg gaggccatca
     1801 cgcagcccga gtcgtggtcc attccattgc gcctggccgt ggccctggcc atcgcgacgg
     1861 tgatcgacct gatcctggtt cacctgctgt tcggatgaga acgcgggctt gcggccgtcc
     1921 gaatcctgag aagatgtctt gggagccggc agtctgatgc cggggcggcg gagagttatt
     1981 cttcggtctt cagatcgaag agtgcccgaa gcatttcggc gtacacgcgt ccggtgccgg
     2041 cggccgagtc gccggtgagg cgggtgatcg gaccgtggag cagcttcccg gtcacgcggt
     2101 ggagcatctg ttcgatgagt tcgcggtcgt gggctgaggc tttttcgagc ttggggagaa
     2161 ttcgttccag ttcctgccgg ccgacttcgc ggaagtgctt ctcgagggcc gcgatcagcg
     2221 ggccgacgtc ttcgcgcgat tgacggtcgg ccagttcgag cacggcggcc tcgatgatct
     2281 cgtggcactt ggcgagggcc tcctttcgct gggcgaggtg ggcctcggtc acggcctgca
     2341 agtcgtcgat gttgtagagg tagacgcaat cgtggcggcc gacggcggcg tcgatgtccc
     2401 gcggaagggc gatgtcgatc agcaggatgg gccggtacgc ccgccgggcg ggtatctggt
     2461 cgaaggcctc ggcggtgagg atcggctcgc gagatccggt gcaggtgatg acgatgtcgg
     2521 cttcggcgag ccggtcgatc cagtcggcga aggggatggg ctggacggtg agcggatgcc
     2581 gccggctgag ccgttcggcc acctcgacgg cgcgggcatg ggtgcggttg gtgacccaga
     2641 cgcgctgggg gcgggtctcg atcaggtggg tcagggtcat cttgcccatg ccgcccgcgc
     2701 caaccatcat gaccgtttta tcgccgaagt tcgagaagat ctgcctggcc agatccacgg
     2761 ccgtgctgcc cacgctgagc cggccgatgg agatgcccgt ggtggcgtgg atggtcttgg
     2821 cgaccgccaa ggcgtgctgg aagagggggc ccaggtttcg gccgacggtt ccggccatgc
     2881 gggcggtctt gaaggcgtcg cgcgtttgcc cgacgatctg cgactcgccg aggaccattg
     2941 aatcaagcga gctggcgacc cgcaggaggt gccggaccgc ctgcacgtct tcgtggatgt
     3001 acagggtgtc ggcgaagtca tgcatttcga tgccgtgaaa ctcgccgatg aaagcgatca
     3061 tctcgtcgat acggggccgg ccctgcatcg gtcgcgagac gtagaactcg acccggttac
     3121 aggttgacag cagcaccgct tcggcttcgg ggaatcgctg cttgaaacgt tggagggcgg
     3181 ccggcaccgc ggcctcgtcg aaagcgattt tctcgcgctt ttcgaccggt gcggaacggt
     3241 gattgcagcc gacgacgaac gttctcatgc gtgcatcagc gagaaaaaca cccggaacag
     3301 atacgcaacg acgatcagac ccaatccgta agtagacaat acagcggccc gcttgccgcg
     3361 gatttgcggc ttatacaacg acccatagca caagtagccg aaaacggccc aagccagcag
     3421 cgatgagatg aagaccgggt ccaggtacca gtcgtcggcc tgcaggtcag gccggtgtga
     3481 aacgccgcac agaccggtca gaatcccata cgtgaaaaac ggaaacccga tgatcggcgt
     3541 ccacctgccg aaccgttcga gggattcgag ggaagcgacg ttgccgacca gggtcgtggg
     3601 ttgcttgcgt cgcaacatcc ggttcacaag cagataggcc acaccggcga cgccgccggc
     3661 aatgaacagc gtcccgctga tcgcaaaggc gatcgagtgg ctgacaaacc aggcacggcc
     3721 ggtgatgcgc atttccggct gattcacaat cgccaaagcc gcacattgga gggcggttgc
     3781 gatgatgaac aggaagccgt ccacgccgcg caaggtgcga gcgaggtgtg tgctgatgcc
     3841 catcaggccg agcagcgttg ccagcaggac ggcgtcatcg aagctgtacc tgaatgtctc
     3901 gacgacgccc atgctccgga atcggcctgc gagatatccg acgtttgcag ccagggcgag
     3961 ggtgaccgcg acccgcagag ccgccggggc ccagcggaca tggtgtctga ggcgcaggta
     4021 cgaacagatc gccccgatcg cgaaccccat tccgccaagg atgcatgtca ggagttgcgt
     4081 catggtgtat tcctgcgggc agcgtggttg tcattgaccg ggccgatcgc ggacctgcga
     4141 ccgggaactg acaacttccg ccgatttgat tagcatacct gaggttgaga gggatttcga
     4201 ttcctcgtga gggacggcgg atgcgaggcg caaggtttgt cggccgggtc ggtttgttgg
     4261 tgctcacaat cgcgacgtcg cctgcggtgg tcagggcgga tgcggattcg gcgcccggcg
     4321 gccgggcggc tgaggctcag tctgatgata tacgacgcgt ggtcgcgtta ctggggcacc
     4381 ctgttccttc gcagcgacag gccgcccggc ggcagttggt ccagtggggc cttcgagtta
     4441 ccgacgaact gcggcgtgtg gcggagggac cgaatcttga ggcggctttt caggcccgcg
     4501 acttgctgga ggccctggat tcggcgttct ttcagggtgg gcagatcagc gtggaggcgg
     4561 atcgggcaga ggtcgattgg gatgagccct ttgcgttgac ggtgacggtt cgcaacccca
     4621 ccagggggcc gttgaaggtg acctgggctg ctccgacgac cgcgcccgcg agcggcccgg
     4681 ccgatgaggc ggcgcaggtt gcggccctgc tcgatgttgc ggatttcctg gttgttcggg
     4741 ggccggacgg gcaggaggtg gatgtgcgga tcgatccgat cgagcgagac ccggttgttc
     4801 gagcggtggt tgaacggcga gcttccggca cacagcccac tcacctggtg ggaccgggcg
     4861 gcgaggcgcg gctgcggatt ccggagttca atcgcggatg ggcccgatat ccgaccttgt
     4921 ctcgcggcaa gtatcagatt tcgattcgcc atcagcctgc gtggaaggat cagaattggg
     4981 tggaggaagg gctcgggctg attgaaagtc cgccgatcga agtccgcgtg cgcagcgggg
     5041 cgccagcgtc gattcgtcat gccaaccggc cgatgcggct gcaactgaag cagacaggcg
     5101 gcgagctgtt gatacagatg cgaaaccttt gggatcgccc ccagtgggtc aaccttgatt
     5161 tcggtgacga catggaagtc caggccaggc tgacctggca ggcgcatcgg ctgacgctgt
     5221 tcggcgatga agatggagtg gagctggagc cgcgagtgaa agccgtcgag cgccgcgagc
     5281 atctcctgcg gctggcgccg gacgaagcga ccgaggtggg ccgggtttcg atgggcgatt
     5341 tccgcgaggc gctggttaag gccgggggtc gccgatccgg gcggtatgcg gtcaccgtcc
     5401 gttaccagtt tctgagcgaa gtcgaaaact tacgcgcggc tcttgccgcc cgcaagagca
     5461 agatcgaagt cccgactcat ctgtataccg gggtcatatt ctgtcgcgag gacgtcaacg
     5521 tgcaggacga ggcgaagatc attggccgac aagatgccgc gccgtcgaag cccagtgaac
     5581 catggcgaat ggcgaagccc gaatgacgca ggaatggcga agcacgaagg acgaagaaaa
     5641 ccagaatggt ttcccgcttg cgatcgggtt tcttaaagga ctggccagaa tgtcacggag
     5701 cagtcacgga ttggtcgggc gggaggcagg gaggctctgt gccgggggcg gggcggtgat
     5761 ccggatcggc tcgcgtcgca gggtgtcaag gcgatcgtca tccttgccgt acttgatcgt
     5821 tacggtcgtg ccggtgcttt ggccgtagag ggaggtcgcg gcaagcagca tcgccggcat
     5881 cgtctgcttg aggtttgaat cataggcgat gaggtccgcc cggcattccc agaggacttt
     5941 tcttcccgcc ggcagtccct ggtcggcgat ccacgccgac agactgagca tgtggtgata
     6001 gccgaggtca accgtccgca cctcggtacc cacacagtca aacagggggg cgccgaacgg
     6061 gccgaacccg tagaccgggc ggaattcggg gtaggtctcg atcgcctggc cgcggtacag
     6121 gacggtgtag gccatggtca tctgcaggtt ggcggtttgg ccgatcggga ctcgtcggag
     6181 gaccggtcgc tgttgtctca aggctcgggc cagcatggcc gagtagtcct cgaactccag
     6241 cgtcgattcg atttgcggcg tgggaccatg gatggcaaag gcgggtccgt cgaggcgatg
     6301 ccagagcgag ttgtcgcttg tcaggcgaca atccacgtgg acgcggtacg gttcacaacc
     6361 ggcggcggcg attgccgcga tcgcaactgc cataccagcc gagcgcatca cagatcctcc
     6421 tttgacggtt gcctattgtc gttgctccat cggtacgtag tgccgcagcg gcgggccgac
     6481 atagatctgc cgcgggcgat agatgcgcgg gttcggactc aggtggacct cacgccaatg
     6541 ggcgatccag ccgggcatgc gaccgatggc gaacatgacg gggaacatgt ccaccgggat
     6601 gccgatggcc cgcaggatga tgccgctgta gaagtcgacg ttcggataga gcttacgttc
     6661 gaggaagtaa ggatcgctca gtgcgatttc ctcgagctgc ttggcgatat ccagcagggg
     6721 gtcgcgaata cccagttggg ccagaactct gtcgcaggcg ctcttgatga tcctggatct
     6781 cgggtcgaag ttcttgtaca cgcggtgtcc gaagcccatg aggcggatgt tggacttctt
     6841 ctccttgacc ttggcgatgt atgacttgag gtcctcgccg ctctgttgaa tgccgagcag
     6901 catctgcacg acggcctggt tcgccccgcc gtgcagccgg ccccagaggg cacagatgcc
     6961 cgcggccacg gaggcgtaga gattggccag gctcgaaccg accatccgga cggttgaggt
     7021 ggagcagttc tgttcgtggt cggcatgcat gatcaagagc aggttcaatg ccgcaatgat
     7081 ctccggggag ggttcgtagg gggcgttggg cttgctgaac atcatgtgca gaaaattggg
     7141 cacatagggc agatccggcc tggggtacat catcggatgg ccgatggact tcttgtagct
     7201 ggccgcggcg atggtgcgca ccttgctcat gatgcgggct gcggcgtcga tgaagtgggt
     7261 gtcatcctcg ggttgcagga ggtcgggctg gtagcagccc atggcgttca tcattgccga
     7321 gaggatggcc atcgggtggg cgctgggggg gaagccctcg aaatgattgc ggaggtcttc
     7381 atggatgaac tcgtgctcca tcagcaggga gcggaattcg gccaactgtc tggaggtggg
     7441 cagctcgccg aaaatgagca ggtaggcgac ctcgacgaaa ctcgactttt cggcgagttc
     7501 ctcgatcggg tagcctcgat atcgaagaat gcctttctcg ccgtcgatga aggtgatgct
     7561 gctcaggcac gatccggtgt tggcgtagct gtcgtcaagc gtgatgcagc cggtcatcga
     7621 tcggagtcgg ctgatgtcga tgcccacttc gttctctgtg ccgatcacaa cgggcaactc
     7681 gtacgacttg ccgtcgataa tcagggttgc ggtcttgttc atgctgatgc ccttgcctgt
     7741 gcgcggccgc cggcagccgt cgcatgcgta tcctcgtcca atgttcgtgc gaacggccga
     7801 tccggccctc aggggccgcc gcttcgagcc cgccgctgat accagtggca ccggtccccc
     7861 aaaccggctc gtgggttcac cggagcgggg ggattgtatc agactccgtc gaatctgtga
     7921 aacgggcggg ggccgtttcc gtggaagctt gactggcagg gcggtcgccg tgaacatggc
     7981 gagtcgatcg cctgcggcgt tcgtctttgg atgtattggg tttatcgagg agtccgccat
     8041 gtccgagttg caggggcggg tggccattat cagcggcggt ctgggtgata tcg
//
LOCUS       DAOTPW010000261         8021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8036_length_8021_cov_5.587371, whole genome shotgun sequence.
ACCESSION   DAOTPW010000261 DAOTPW010000000
VERSION     DAOTPW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8036_length_8021_cov_5.587371"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1085)
                     /locus_tag="P5316_18415"
     CDS             complement(177..1085)
                     /locus_tag="P5316_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86988.1"
                     /translation="MRLLEPLAELRDRLRGYPPWVLVLAAPLYPLVFFGCLICLPLLL
                     PNWILGPLLGPLVAPIGRSRAVQWLSTKKVEDFPVYVAELAGLFHWDVARAADLLQRI
                     RVVRRILLLIIGGQWVALLATGVIVGVSFALREPPDLVGLAVIACGAILVVSTVFSAS
                     GLSYYHGLYKSWQTLQTALCGQCGYIREHVQSLRCPECGTGEPAVPPGRAPRCRDVWS
                     PLVNKWTTGLPLSLLCCMFLFDRLVLDACLSAKTHGWVVLMMTGSAAGVILWVLWVFW
                     FRWKRAWSRSPGDKRGACVESDGTGW"
     gene            complement(1256..1762)
                     /gene="coaD"
                     /locus_tag="P5316_18420"
     CDS             complement(1256..1762)
                     /gene="coaD"
                     /locus_tag="P5316_18420"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRR86989.1"
                     /translation="MSQRKPARAVFPGAFDPITHGHLDVIKRAREFVDELIVAVGHNP
                     EKRELFTAQERVDMLNELVGDLPRVRVEAYEGLTAEFVRRVGAQMIIRGIRDNVDLHY
                     ELQQANINMAVGGVETIFLLTRDQFALVSSTYIKQIVELGCTDLERLSRLVPPNVAER
                     LRLKHGSQ"
     gene            2439..4073
                     /gene="cimA"
                     /locus_tag="P5316_18425"
     CDS             2439..4073
                     /gene="cimA"
                     /locus_tag="P5316_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRR86990.1"
                     /translation="MSTPRQRIEIYDTTLRDGTQSHNVSLSLNDKLMIATKLDSLGVD
                     YIEGGYPLSNARDAAFFQEVRRLKLSHAKVTAFGMTRRRGTKADKDEGLKALLDSEAP
                     VITVVGKTWDMQVRDVLQVSEQENLDMITESVAFLTRQGREVLYDAEHFFDGWADNPD
                     YALKTLLAAQEAGASCLVLCDTNGGSLPEQIAKAVDAIRPHLRAKLGIHTHNDAGLAV
                     ANTLTAIQHGAVHAQGTINGIGERCGNVDLTTVVANLRIKMNKAVLHDDGLKRLTEVS
                     RYVYEVLNLVPPDNQPYVGPFAFAHKGGMHVHAIRRVSRSYEHIDPMLVGNERRVLIS
                     ELSGASGVAEKIGRKLDVAHDKALQRRLLKRLQEYEEQGYVYESAEASFELLCRKELG
                     LYHPFWQLDHWRAVALKSPQFHEPDTEVIVRLRINGTLEHHVSGGHGPVDAMANALMK
                     ALRPHYPEVDQVRLIDYKVRVINAKAATAARVRVTIEFRNEVTHEIYGTVGVSENIIE
                     ASWIALVDGIEYSLINHEEMTREESGRRTTKARRPK"
     gene            complement(4161..5693)
                     /gene="murJ"
                     /locus_tag="P5316_18430"
     CDS             complement(4161..5693)
                     /gene="murJ"
                     /locus_tag="P5316_18430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRR86991.1"
                     /translation="MSKVLQSAKLISVLTLVSRILGLVRDIVCAHAFGTEVVWSAFRM
                     GFQIPNLFRRLFGEGALSAASIPVLTETLARDSKGATDVLAGRLLGLQLAVLAGLCII
                     GECVVAGLYWVYGGDSDNALTFALTGLMLPYMIFICTAAVLGGVQNVFGRFASPAAAP
                     IILNIFMIAAAWLAPRVVPGGMRRQIVLLAAAVVVSGIFQLAWQWLSARQCGLRLPLR
                     INTRDPNIRRICVTMLPMMGGLAVVPISSLLDNLIAYWFVPERVGPAILGYAQELYQF
                     PLGVFAIAMATAIFPTLAKHVADNDIPGMKATLARGIRVVEFEALPCMVGLILVRDPL
                     VRTLFERGQFAENPDSAPRVAFALYMYALGIIAYGVNHLLVRTFYAMHDAKTPLRVSV
                     INVVINFAVNLVLVQTALREAAMGLSTSVCAMGQVVALSVLLRRRIGPLAWGEIGRSV
                     TRSAAATVIMALTVLVVDHYVDGSLRPLYRLVILVCTGGIAYLGAAFVLGCQEMREML
                     RK"
     gene            5925..6761
                     /locus_tag="P5316_18435"
     CDS             5925..6761
                     /locus_tag="P5316_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HRR86992.1"
                     /translation="MMSIGGFRFLRGRQHGFTLIEVLVVVAIIALLIAILLPSLARAR
                     ELAKRTQCGANTHQMGIALHMYTLEHRHYPGHHLTYGRSDILWPVRLNRFMRQPEIFW
                     CPSAPADTHWNGIDRIYRDIAAAQPEEQATFAYGYNDWGVAETIGRNPPVYNYGLGGH
                     INHPYEGEVRVERVKRPSDMIALGDSDSGSPNPKAKSGIWDTAIDPIDDNGLEWPGDR
                     HLKGACIVFADGHSEWLLQSRLVEGTIGMRKKWNNDYRAHYDDWGDKYKFPIANVPPE
                     ER"
     gene            6798..7139
                     /locus_tag="P5316_18440"
     CDS             6798..7139
                     /locus_tag="P5316_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR86993.1"
                     /translation="MKRILIYAGLGAFIVAAGLYTYIKVSAAVPAQADGFHLKCTSAE
                     CGRYFTIPRKQVRTYPRDPNGQGFRCEKCGKFSARIAGRCEQCGEWYAVTQDVGREGG
                     CPKCAGRQITK"
     gene            complement(7234..7449)
                     /gene="iscX"
                     /locus_tag="P5316_18445"
     CDS             complement(7234..7449)
                     /gene="iscX"
                     /locus_tag="P5316_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HRR86994.1"
                     /translation="MPPLKWTDAEDIGIRLFEKYPNEDPLAVRFTDLHKRVCELEGFG
                     DDPKASNEAHLEAIQMAWLEEYRDGKE"
     gene            complement(7547..7954)
                     /locus_tag="P5316_18450"
     CDS             complement(7547..7954)
                     /locus_tag="P5316_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRR86995.1"
                     /translation="MDAKNPRLTDAAQRRPLRKYRITFLPSGRTVEVDPGETDHAADG
                     LPWSILDIGLRHGVEIDHACGGVCACATCHVIVRQGLGSCGDPEEEEEDRLDNAYGLT
                     PQSRLACVCVPDGSSDVVVEIPAWNRNRVGESR"
BASE COUNT         1850 a         2576 c         2249 g         1346 t
ORIGIN      
        1 gcaaccatcc ccgtaggaca tgttcgcagc cgccagatca cgtagggcac ggtcgcagcg
       61 gccaaaggcc gcgcagacct gccgcaacca tccccgaccg ggataacctc gacaacagac
      121 aaccgtagcc aaggacagag ggaggaatgg caggtctcgc tgcgctcgat actgccctac
      181 cacccggtac cgtcgctttc cacgcatgct cctcgtttgt cgcctgggct ccgggaccac
      241 gctcttttcc accgaaacca gaagacccag agcacccaca gaatcacgcc tgcggctgat
      301 ccagtcatca tgaggaccac ccaaccatgt gttttggcgc tcaggcatgc gtctagaacg
      361 aggcggtcaa acaagaacat gcagcagagc aggctgagcg gcaggcccgt ggtccacttg
      421 ttgaccagcg gcgaccagac atcgcgacac ctgggtgcgc gcccgggagg aacggcaggc
      481 tcgccggttc cacattccgg gcacctgagg ctctgcacat gctcacggat atacccacat
      541 tggccgcaaa gagcggtttg gagagtctgc catgacttgt acagcccgtg gtagtagctc
      601 aggcccgatg ccgagaagac cgtcgacacc acaaggattg cgccgcaagc tatcacggca
      661 aggccaacca agtccggagg ctcgcgcaac gcgaatgaga ctccgacgat gactccagtg
      721 gcaagaaggg ctacccactg tccaccgatg atcagcaaca gaattcgtct gaccacacga
      781 attcgctgca acagatcggc cgctctcgcc acatcccaat gaaagagtcc tgccaactca
      841 gcgacataca cggggaagtc ctcgaccttc ttggtcgaga gccattgcac tgcacgggaa
      901 cggccgatcg gggcaacaag aggaccaaga agcggaccga gaatccagtt cggcagaagc
      961 aacggaagac atatcaagca gccgaagaac accagtggat acaacggagc ggcaagcaca
     1021 agcacccagg gaggataacc ccggagtcga tctcgaagct cagccagcgg ttccaggagc
     1081 cgcattccag gaaacctcgc accgattgcc tccgaacagc attccgcaat gtaattgctg
     1141 actcacccga gatcgagcac cccgctcgcg catcgcctga tttgagatct gagattgcca
     1201 gatttcaaat cgtccgaatg atggtccgcc ggggcgctcc ctaccccttc gtgtctcatt
     1261 gcgaaccgtg cttcagccgc aaccgctcgg ccacattcgg cggaaccagc cgcgaaagcc
     1321 tttccagatc cgtgcaaccc agttcgacaa tctgcttgat gtaagtgctg gaaaccaagg
     1381 caaattggtc gcgggtgagc aggaagatcg tctccacccc ccccaccgcc atgttgatgt
     1441 tggcctgctg aagctcgtag tgcaagtcga cgttgtcgcg gatgccacgg atgatcatct
     1501 gggcacccac ccgccgcacg aactcggccg tcagcccctc gtaggcctcc acccgaaccc
     1561 gcgggagatc gccaaccagt tcgttaagca tgtcgacccg ctcctgagct gtaaatagct
     1621 ctctcttctc ggggttatgg ccgacggcga caatcaactc gtcgacaaac tcccgcgccc
     1681 gcttgatgac gtcgagatga ccatgagtga tgggatcgaa ggctcccgga aacacggcac
     1741 gagcgggttt gcgttgcgac ataggacgat ttcctgggac agatgaccaa cagactgaaa
     1801 gtgtcctgaa aaagtatggg acgggcttcc agtcgaccat cggaccggca agatgccggt
     1861 cccacaccgg gatctcagga cgcacaacaa gccgcaagaa ttgtaaccca cccggccgca
     1921 ctcccacaac ggggcccttg ccaacgactg gccccgatgc tagcataaag ttatgtcgaa
     1981 cagaatggcc gagtcttatt gccttgcccg ccggcatacc gcagcgatgc ggatcggccg
     2041 tatcatcggc attattcgag gagaacccgg gtagggccgc gtgaggcgaa cgcgatcatg
     2101 acagccctgc ccggtgaggc agggcttttt gttaggccgc taggctatta gaaccttatg
     2161 gccggatatg tgtgcggctt gcgcgcatgt ttctggatcg cagggcgctt gggttgcggt
     2221 ccgcaaaaag gcggtccgcc ttggactgtt cggccgttag acctttagga tgctgccagc
     2281 agtgaattca ccactcctcc gctgaagcag cggagcacct gcgtgattct tcctaacagt
     2341 ctaacagcct gacagtcgaa tagtctaatg gtctgacacg cacggcatcg ggcaaaagct
     2401 cgaccaagaa agcagacacc aaacagacga gagaccatat gtccacaccc cgacaacgca
     2461 tcgagatcta cgacacgacg ctccgcgacg gcacgcagag ccataacgtg agtctgagcc
     2521 tgaacgacaa gctcatgatc gccacgaagc tcgacagcct tggcgtcgat tacatcgaag
     2581 gcggctaccc cctcagcaac gcacgggatg cggcgttctt ccaggaggtt cgacgactca
     2641 agttgtcgca cgccaaggtg acggcattcg gaatgacccg ccgccgcggc acgaaagccg
     2701 acaaggacga ggggctcaag gccctcctcg actccgaggc gcccgtcatc accgttgttg
     2761 gcaagacatg ggatatgcag gttcgtgacg tgctccaggt cagcgagcag gaaaacctcg
     2821 atatgatcac cgagtccgtg gcttttctca ctcggcaagg tcgtgaggtt ctctacgacg
     2881 cggagcattt cttcgacggg tgggcggata accccgacta tgcactcaag actctgctcg
     2941 ccgcacagga ggcgggggct tcatgcctgg tattgtgcga cacgaacggc ggaagcctgc
     3001 cggaacagat cgccaaggcc gttgacgcca tccgaccaca cctccgcgca aagctcggca
     3061 ttcacacgca caacgacgcc ggcctggcgg tcgccaacac cctcacggca atccagcacg
     3121 gcgccgtcca cgcccaggga accatcaacg gcatcggcga gcgatgcgga aacgtcgacc
     3181 tgaccaccgt cgtggccaat ctcagaatca agatgaacaa agccgtcctg cacgacgacg
     3241 gccttaaacg gctcactgag gtcagccggt acgtctacga ggtgctcaac ctggtgccgc
     3301 ccgacaacca gccctatgtc ggcccgttcg ccttcgccca taagggagga atgcacgtcc
     3361 acgccatccg ccgggtcagc cgcagctatg agcacatcga ccccatgctg gtgggcaacg
     3421 aacggcgggt actgatcagt gaactcagcg gggccagcgg tgttgccgag aagatcgggc
     3481 gaaaactcga tgtcgcccac gacaaggccc ttcagcgtcg gttgctcaag cgactccagg
     3541 agtatgagga acagggttac gtatacgagt cggccgaggc cagcttcgag ctgctctgcc
     3601 gtaaagaact gggactctat cacccgttct ggcaactgga ccactggcgg gcagtggccc
     3661 tgaaatcacc ccaattccac gagcccgaca ccgaggtcat cgtcagactc aggatcaacg
     3721 ggaccctcga acatcacgtc tcgggcggtc acggcccggt cgatgccatg gccaacgccc
     3781 tcatgaaagc tctccggcct cattatcccg aggtcgacca ggtgcggctg atcgactaca
     3841 aggtgcgcgt catcaacgcg aaagccgcca cagcggctcg cgtgcgcgtc accatcgaat
     3901 tccgcaacga agtaacccac gaaatatacg gcacggtcgg tgtcagcgaa aacatcattg
     3961 aggcgtcctg gatcgcgctg gttgacggca tcgaatactc cctgatcaac cacgaggaaa
     4021 tgactcgcga agaaagcggc aggagaacaa ccaaggcgag aagaccgaag taggagagtc
     4081 gtcagtgctc agtgatcagt tgtcagtcgg acagtgtgct ccgagggttt gactgaaaac
     4141 tgacgaccga tcactgatca ctacttccgc agcatctccc gcatctcctg gcatcccagg
     4201 acgaacgccg cgccgaggta agcgatcccg cctgtgcaga ccagaatcac caaacggtaa
     4261 agcggcctca ggctgccgtc gacatagtgg tcgaccacca gtactgtcaa ggccatgatc
     4321 acggtcgcgg ccgcgcttcg agtaacgctt cgtccgatct cgccccaggc aagcggaccg
     4381 atccgccgtc tcagcagcac agagagtgcg accacttggc ccatcgcaca caccgaggtc
     4441 gacagcccca tggcggcttc ccgcaaagct gtctgaacca ggaccagatt gacggcaaag
     4501 ttgatcacca cgttgatcac cgacacccgc aacggcgtct tggcatcgtg catggcgtag
     4561 aaggtgcgca ccagtaggtg attaacccca taggcaatga tacccaaggc atacatgtac
     4621 agggcaaatg ctacacgcgg cgccgaatcc gggttctctg cgaattgccc tcgctcgaat
     4681 agcgtccgca caagcggatc ccgcaccaga atcagaccca ccatgcacgg caaagcctca
     4741 aactcgacca cgcgaatccc gcgagccagc gtggccttca tgcccggaat gtcattgtcc
     4801 gccacgtgct tcgccagcgt cggaaagatc gccgtcgcca tcgcgatcgc aaaaaccccc
     4861 agcgggaact ggtacaactc ctgggcatag cccaggatcg ccggccccac acgctcggga
     4921 acgaaccagt aggcgatcag gttgtccaac aagctgctga tcggaaccac cgccagaccg
     4981 cccatcatgg gcagcatcgt cacgcaaatc cggcgaatgt ttgggtcccg cgtgttgatc
     5041 cgcaaaggca aacgcaaccc gcactgcctg gccgacagcc attgccatgc cagttgaaag
     5101 atccccgaaa cgaccacggc cgccgccagc agcacgatct ggcgccgcat gccgcctggc
     5161 accactcgcg gagccaacca tgccgcggca atcatgaaga tgttcaggat gatcggtgcc
     5221 gccgccgggg atgcaaaccg accaaagacg ttctgcaccc cgccaagcac ggcggcggtg
     5281 cagatgaaaa tcatgtaagg gagcatcagg ccggtcaggg caaatgttag ggcgttgtct
     5341 gagtcgccgc cgtaaaccca gtacagaccg gcgacaacgc actcgccgat gatacacagg
     5401 ccggccagca ccgcgagctg aaggcccagc agccgtcccg ccagaacatc cgttgccccc
     5461 ttgctgtccc gtgccagcgt ctcggtcagc accgggatgc tcgccgccga cagcgctccc
     5521 tccccaaaca gccgccggaa aagattagga atctggaaac ccatccggaa agccgaccac
     5581 accacctccg tgccgaacgc gtgggcgcag acgatatccc gcactaaccc cagaatgcgc
     5641 gaaaccagcg tcagtacgct gatcagcttg gctgactgta gaaccttcga catcggtcca
     5701 gagaatatcg acagaacagg cactcaaaga acaagagtcc ctgccaaaac cacactggga
     5761 ccggcttccg taacagatgg ccacgccgcc cccgctctct cgaggccccg cccctatcac
     5821 gttctttatt ccagggtatt gggtcccacc gagttgaatg gtatgataaa caccgcgccg
     5881 atagaacgct ggcagccgga ctgtgatccg tttcgaggag gccgatgatg agcatcggcg
     5941 gctttcgatt cctgcgcggt cgtcaacacg gtttcaccct gatcgaagtc ttggtcgtgg
     6001 tggcaatcat cgctctgctg atcgcgattc tgctgccgtc cctggcccgg gcccgcgaac
     6061 ttgccaagcg gacgcagtgc ggggcaaata cgcaccagat gggcatcgcc ctgcacatgt
     6121 acacgcttga gcaccggcac tatcccgggc accatctcac atacggacgc agcgatattc
     6181 tctggccggt tcggctcaac cggttcatga gacagcctga gatattctgg tgcccgtcgg
     6241 cccccgctga cacccattgg aacggcatcg atcgaatcta ccgcgatatc gccgccgccc
     6301 agcctgagga gcaggcaacc ttcgcctacg gctacaacga ctggggcgtc gccgaaacca
     6361 tcggccggaa tccgccggtc tacaattacg gcctcggcgg ccacatcaat cacccttacg
     6421 agggcgaggt gcgagtcgag cgagtgaaac gcccgtccga catgatcgcc ctcggtgaca
     6481 gcgactcggg cagccctaac ccaaaagcca aaagcggcat ctgggacacg gcgatcgatc
     6541 ccatcgatga caacgggctg gagtggccgg gagaccggca tttgaagggc gcctgcatcg
     6601 tcttcgccga cggccatagt gaatggcttc tgcaatcgag gctggtggaa gggaccatcg
     6661 ggatgcggaa gaagtggaac aacgactaca gagcacacta tgacgattgg ggcgacaagt
     6721 acaagttccc catcgccaac gttccgcctg aagagcggtg aaaacggact tggaccatcg
     6781 gtcgagaagg tgacgatttg aaaagaatcc tcatctatgc cgggctgggc gccttcatcg
     6841 tggcagccgg cctttataca tacatcaagg tctccgctgc cgtacctgcg caagctgacg
     6901 gatttcattt gaaatgcacc tcagccgagt gcggccggta tttcaccatc ccgcgcaagc
     6961 aggtccgcac ctatcctcgc gatccgaacg ggcaaggatt ccgctgcgag aaatgcggca
     7021 agttctcggc ccggatcgcc ggccgatgcg agcagtgcgg cgagtggtac gccgttaccc
     7081 aggacgtagg acgggaaggc ggttgcccaa agtgtgccgg ccggcaaatc acaaaatgaa
     7141 gatgtgaagc tccgcgtctc gcgtgcgacg cacgacgcac tcggttttcg gacgggcagg
     7201 aaaccattgc tgcgagcccc cgaacggcaa acgtcattcc ttcccgtcgc ggtactcctc
     7261 aagccaggcc atctgtatgg cctcaaggtg ggcctcgttt gaggccttgg gatcatcgcc
     7321 aaagccttcg agctcgcaga cccgcttgtg cagatcagtg aatcgcacgg ccaaaggatc
     7381 ttcgttggga tacttctcga agaggcgaat gccgatatcc tctgcgtcgg tccacttcaa
     7441 ggggggcata ttccaacctt ccattcaaga gaatagtaca ccctggcgac cccgcctgga
     7501 ccggcaacat gcctatccca cactttgatc ccggatggcg atcgaatcag cgactctcgc
     7561 cgactcgatt gcggttccag gccggaattt cgaccacaac atcactgctg ccatcaggca
     7621 cgcagacgca ggccagacgc gattgaggag tcaagccgta cgcgttgtcc agccggtctt
     7681 cctcctcctc ctcagggtcg ccgcacgatc caagaccctg gcgaacgatc acatggcagg
     7741 tcgcacaagc acacaccccg ccgcaagcat gatcaatctc gacaccatga cgcagcccga
     7801 tatccagaat gctccaaggc aggccgtcag ccgcatggtc ggtctcgccc gggtcaacct
     7861 cgaccgtcct gccggatggc aagaaggtga tccgatactt ccgcaagggg cgacgttggg
     7921 ccgcatcggt caaacgggga ttcttggcat ccatcggcga tggttctccg ttcagtgacg
     7981 aggggccgag acaccggtcc gatccgtctc acggagcgtc t
//
LOCUS       DAOTPW010000262         7937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8115_length_7937_cov_5.169881, whole genome shotgun sequence.
ACCESSION   DAOTPW010000262 DAOTPW010000000
VERSION     DAOTPW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8115_length_7937_cov_5.169881"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..2097)
                     /locus_tag="P5316_18455"
     CDS             complement(67..2097)
                     /locus_tag="P5316_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRR86996.1"
                     /translation="MNLHWVDWAIVLSLVAFLILTAQFTRRYMRGVSDFLAANRCGGR
                     YLICISSGGAELGAVTIVALWQLYTNSGFTGLWWKVAEWPLVFILAMSGWVLYRYRQT
                     RALTMAQFLEMRYSRRFRVFTGFVAFAAGLVNYGVFPSINARLIIYFCGLPEYVSLWG
                     LSIGTFPLVMMVLLGLALFFTFAGGQIAIMLTDFVQGICCLVVFLVAALVLYYLIGWD
                     TIGTSLLAGSQPQASLLNPFDTSKIKGFSMGFFLVQYFRWVYHWKAWQGTQGFNAAAR
                     TPHEARMSGILGQWRFIAQQMLIPIFAICVLAALNSPQFADSVGNAKQVLSGIHTDRV
                     KSELTVPLVLARLLPIGLMGAMAAVLLAAAVATDQAYLHSWGSIFIQDVVLPLRKRPL
                     TPQQHIRCLRLAILGVAAFAFWFSLVFRDLGDYVLMFFAATGAIYLGGAGCSIISGLY
                     TRRGTTAGAWGAMITGVVLGGIWLVANRLAIADAGAGGMVFRLQSVLYQSPAIEWFVN
                     TIGKGDRKYLDGQWMCLYISLTAIAVYWILSLLLPGKPFDLDKMLHRGQYAVAEDVVQ
                     GDAALSGIWKWLGVNKEFTRGDKAIYGASVIWVLVWGGIFIAGAIYHCGIRETTDASW
                     LGFWKILVWLALGLGVTVTVWFLIGGVRDLKDMFHLLRTKARDEADDGRVRS"
     gene            complement(2201..3013)
                     /locus_tag="P5316_18460"
     CDS             complement(2201..3013)
                     /locus_tag="P5316_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR86997.1"
                     /translation="MSDTPQEPTIQEMFNLSGKVALITGACGWLVQSMCRALAEAGAG
                     VVCADIPAAREKAQEVAKSLPVVGNARHYVTELDYMDAKSIDNCMADAVKQAGKVDIL
                     VNSGYERVTSDWRTVSGEQFTRQLQNATGVFLLSRAMRQHVVDRKGKGSIINIGSMYG
                     IVGSYPDAYSDVPMCSPVAYHCLKGGILSMSRHMAVYWVGDGIRVNVLSPGPFPAPNV
                     SPKMVERLSSKSPMKRMGKPHELKGALVFLASDASSYVTGANIVVDGGWMAW"
     gene            complement(3189..4205)
                     /locus_tag="P5316_18465"
     CDS             complement(3189..4205)
                     /locus_tag="P5316_18465"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR86998.1"
                     /translation="MAKHRILIIGVGSIGERHTRTFQRTGRAELSICESNEQLRRTVA
                     ERYGINQTYAGLDEALNDDKFDIGLICTPSHLHVPMATRLVKARKHVFIEKPLSLSLY
                     GISELMELTREKGVKAAVAYTYRSHPALRSMKEAIDSGRFGEPVQVIGTWGQHFPKYR
                     PAYRQIYYTSRKTGGGAIQDVLTHAINAGEFLAGPVGRLMADCGHQVLEGVSVEDTVH
                     VITRQNGDKVMGCYSLNQHQAPNENTVTVVCKKGTARWDLNNARWMSMAEPDAGWKTE
                     FQFSTERDDIFVNQANIFMDAIEGKGNFACTLEEGLQTLRVNLTVLQAADKPNWQPLV
                     AVPS"
     gene            complement(4341..5159)
                     /locus_tag="P5316_18470"
     CDS             complement(4341..5159)
                     /locus_tag="P5316_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRR86999.1"
                     /translation="MRRSTVKSKLLRKQPALCLQCHFTDQSVFELVALLGMDGIWMDL
                     EHHNYSVETANGLIRATRVAKCDVVARAAKGEYMRFARLLEAGAHGLMYPRCDDAAEA
                     AELVKWTKFYPIGKRGFDGGNGDMPYCSVPMADYMKAANEETFIIIQLEEQAAVDRAD
                     EIAAVPGVDILFLGPADFTTLSGIPGQWDHPKVWNAYKKIAKAAEKAGKWWGTPAFDT
                     EHCRRLMDLGALFFCHSADIVIFKAGVENIQRQFSPLGFTFDNRLAAGRSYIEG"
     gene            5418..6077
                     /locus_tag="P5316_18475"
     CDS             5418..6077
                     /locus_tag="P5316_18475"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR87000.1"
                     /translation="MSIAPPQNGSLRDQAYYRLRRLLILQQVREGERLRESHWAAKLS
                     VNRTALREAFARLEAEGFVTKGPKTGYFVPALTNEDIREILDVRLILEGGAIERIIAA
                     KNNTPTHLRAMRVACEQLERLVREEYMLGVAEADRRYHEALIEAAGNKRLVTLYQRAP
                     LPIIHPEVISGHLWEVRVQKTLNEHRAILDAILKGSAEEAKAMLGTHLTERAIVPLHA
                     G"
     gene            6135..7796
                     /locus_tag="P5316_18480"
     CDS             6135..7796
                     /locus_tag="P5316_18480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRR87001.1"
                     /translation="MPQTAPPCRKTPLIGCVMIIGGLVVADAAWGQTAAPASSSAAVP
                     YGLTTLDWGIIAAYGAGMLGIGVYYARRTRNREDYLLGGRRMRSSSVGLSLFATLFST
                     ITYLALPGEMISKGPVILWSMMAIPIAYVLVGYFIIPRFMEQRVTSAYEILETRLGVG
                     LRLLAAIVFLVTRTLWMALIIYVTAEKIIVTMMDLPPDTAAWVTPVVAAAVGLLTVAY
                     TSMGGLRAVVLTDVIQSIILLGGACLTIILITVKMGGVTAWWPSAWSPNWDHQPFFSF
                     DPTVRVTVIGSILFMTIWWIGTAGSDQMAIQRYLATRDTKTARRVFLVTGLANILITT
                     LLACVGFALLAFFTAHPDYLSAGALETKADIVFPLYIIHHVPPGLTGLILAALLAAAM
                     SSLSSGVNSACSVISVDFIDRFRKVPAADSDAVQVKRIRIISFVSGFVAVVISLLMGQ
                     IKGMNIMEITVRTNHVFVAPLFMLFVLALFVRFATPIGAACGTLAGCAVAAVFACTNK
                     VSFQWMSLAALLVDLLVAVPVSYWTYKEKPPASGVSAVSRDGVRQ"
BASE COUNT         1502 a         2439 c         2422 g         1574 t
ORIGIN      
        1 acttctgtcc ggacgtgccg gatcacggcg gagccccggg ccgtcgacgt cgcggtggga
       61 gccgcttcac gaacggaccc gcccgtcgtc ggcctcatcg cgggctttgg tgcgaagcag
      121 gtggaacatg tccttaaggt cccgcacacc gccgatcagg aaccacaccg tgaccgtcac
      181 acccagcccc agcgccagcc agaccagtat tttccagaag cccagccacg aggcgtcggt
      241 tgtctcgcgg atgccgcaat ggtagatcgc accggcgatg aagataccgc cccagaccag
      301 cacccagatg accgaggccc cgtagatcgc cttgtcgccg cgggtgaact ctttgttgac
      361 gcccagccat ttccagatgc cgctgagcgc ggcgtcgccc tggacaacat cttccgcaac
      421 ggcgtattgc ccgcggtgca gcatcttgtc gaggtcgaag ggctttcccg gcaggagcag
      481 agacagaatc caatataccg cgatcgccgt cagggagatg tataagcaca tccactggcc
      541 atccagatac ttgcggtcgc ccttgccgat ggtattgacg aaccactcga tcgccggaga
      601 ctgatacagc acggattgca ggcggaacac cattccgccg gcgccggcat cggcgatcgc
      661 gaggcggttc gcgacgagcc agatgccgcc aaggaccacg ccggtgatca tggcccccca
      721 tgccccggcc gtggtgcccc ggcgcgtgta aagaccggag atgatggaac acccggcgcc
      781 gcccaggtag atcgccccgg tggcggcaaa aaacatcagg acatagtcgc ccaggtcgcg
      841 gaacaccagg ctgaaccaga aagcgaaggc ggccactccc aggatagcca gcctcaggca
      901 gcgaatgtgc tgctggggag tgagcggcct tttgcggagt ggcaggacga catcctgaat
      961 gaaaatgctg ccccatgagt gcaggtaggc ctgatcggtg gccaccgccg ccgccagcag
     1021 gaccgcggcc attgccccca tcagcccgat cgggagcaac cgggcgagaa ccaggggaac
     1081 ggtcagctcc gatttcaccc gatccgtgtg aattccgctc aaaacctgtt tcgcatttcc
     1141 cacgctgtcg gcgaactgcg ggctgttcaa ggccgccaac acgcagatgg cgaagatcgg
     1201 gatgagcatc tgctgggcaa tgaagcgcca ctgtccgagt atgccggaca ttcgggcttc
     1261 gtggggagtg cgagcagccg cgttgaagcc ctgggttccc tgccaggcct tccagtggta
     1321 cacccaccga aagtactgca ccaggaagaa ccccatgctg aagcctttga tcttgctggt
     1381 atcaaacgga ttgagcaggg aagcctgggg ctgtgacccg gccaacagag atgtgccgat
     1441 cgtgtcccag ccgatgaggt agtaaagcac cagagccgcg accaggaaaa ccacgaggca
     1501 acagatgccc tggacgaagt cggtcaacat gatggcgatc tgcccgccgg cgaaggtgaa
     1561 aaacaacgcc agacccagta gcaccatcat caccagcggg aacgtcccga tcgacagacc
     1621 ccacagactc acgtactcgg gcaggccgca aaagtagatg atcagccggg cattgatgct
     1681 cgggaacaca ccgtaattga ccagcccggc agcgaaggcc acgaagccgg tgaacacgcg
     1741 aaaccggcgg ctgtagcgca tttccaggaa ctgggccata gtcagggctc gggtctggcg
     1801 gtagcggtac aggacccacc cggacatagc caggataaac accagcggcc actcggccac
     1861 cttccaccac agcccggtga acccgctgtt ggtatagagc tgccacaggg ccacgatcgt
     1921 gaccgcgcca agctccgccc cgccggagga gatacagatc aggtatcgtc cgccgcagcg
     1981 gttggccgcg aggaagtcgc tgaccccgcg catgtagcgc ctggtgaact gggcggtgag
     2041 aatgagaaag gcaaccagag acagaacgat cgcccagtcg acccagtgca ggttcataag
     2101 ggcacctcag gcgacgtgcc gagttgcgtc aagccgaaac acattcgcgc accggcgagc
     2161 agccgccggt ggcaccccgt gccggtgctc ctttttcagg tcaccaggcc atccagccgc
     2221 cgtcgaccac gatgttcgcg ccggtgacgt agctgctcgc gtcgctggcc agaaagacca
     2281 gagcgccctt gagttcgtgg ggtttgccca tgcgtttcat cgggctcttg ctgctgaggc
     2341 gctcgaccat cttggggctg acgttcgggg ccgggaacgg gccggggctc aagacattga
     2401 ctcggatgcc gtcgcccacc caatagaccg ccatatgccg gctcatggac agaattccgc
     2461 ccttgaggca gtgatacgcg accgggctgc acatcggcac gtcgctatag gcatccgggt
     2521 atgacccgac gatgccgtac atcgagccga tgttgatgat gctgcctttg cctttccggt
     2581 cgacaacgtg ttgtcgcatc gcccggctca ggaggaacac accggtagcg ttctggagtt
     2641 ggcgggtaaa ctgctcgccg ctgaccgttc gccaatccga ggtgacccgt tcgtagccgg
     2701 aattgaccag gatgtcgacc ttgcctgcct gcttcaccgc gtcggccatg cagttgtcga
     2761 tggacttggc gtccatgtag tcgagctcgg taacatagtg tctggcgttg ccgacgaccg
     2821 gcagcgactt ggccacttcc tgggcttttt ctctggccgc ggggatgtcg gcgcagacca
     2881 cgccggcgcc ggcctcggcc agggcccggc acatcgactg aacgagccag ccacaggctc
     2941 cggtgatcaa tgcgaccttg ccgctgagat tgaacatttc ctggatcgtc ggttcttgtg
     3001 gggtatcgct cattgcatcg gctccttcgt gtctcgagga cccgaatgag gagaaaacca
     3061 agaggcgcag tcagtgatcc gttgtcagtc gggagtcaga ggcacatcgt gactcttgaa
     3121 tcgaacaccg gtaacccgtc actgacgact tgccttcctc attcggactc cctcactcat
     3181 cattttgttc agcttggcac cgcaaccaga ggttgccagt tcggcttgtc cgccgcctga
     3241 aggacggtca gattcacgcg cagcgtctgc aggccttcct ccagcgtaca ggcaaagttt
     3301 cccttgcctt ctatggcgtc cataaaaatg ttcgcctggt tgacgaaaat gtcgtcccgc
     3361 tcggtggaga actggaactc ggttttccag ccggcgtccg gttcggccat ggacatccag
     3421 cgggcgttgt tcagatccca tctggcggtg cctttcttgc agacgacggt gaccgtgttt
     3481 tcgttgggtg cctggtgctg gttcaggctg tagcagccca tgaccttgtc gccgttctgg
     3541 cgggtgatca cgtgaaccgt gtcctcaacg ctcacacctt cgagcacctg gtgcccgcag
     3601 tcggccatca gtctgccgac cgggcccgcg aggaactctc cggcgttgat tgcgtgggtg
     3661 agcacgtcct ggatggctcc gccgccggtc ttgcgactgg tgtagtagat ttgccggtac
     3721 gccgggcggt acttgggaaa atgctgcccc cacgtgccga tgacctgcac gggttcgccg
     3781 aatcgcccgg aatcaatggc ctccttcatc gaccgcaggg caggatgcga gcggtaggtg
     3841 taagccaccg ccgctttgac gcccttctcc ctggtcagct ccatcagttc cgaaatcccg
     3901 tacaacgaga gactgagcgg cttttcgatg aagacgtgct tgcgtgcctt caccaacctg
     3961 gtcgccatcg gaacgtgcag gtgcgagggc gtgcagatca ggccgatgtc aaacttgtcg
     4021 tcgttgaggg cctcatcgag tccggcatag gtctggttga tcccgtatcg ttcggccacg
     4081 gtcctgcgaa gctgctcgtt gctctcgcag atggacaact cggcccggcc ggtacgctga
     4141 aacgttcggg tatggcgttc gccgatcgac cctaccccga taatcagaat ccgatgcttc
     4201 gccatgaaca acgttctcca tcgccgcctg ccaccggccg caggggccga attccgggcg
     4261 tgaccggcat tgtgccggtg ctcctcagtt ctccgtttgc gacttctcaa ctccctgcat
     4321 tgcggtctgc atcgtcgatc tcaaccttca atgtaactcc tgccggcggc caggcgattg
     4381 tcgaaagtaa aaccgagcgg cgagaactgc cgttggatgt tctcgacgcc ggctttgaag
     4441 atgacgatgt ccgcgctgtg gcagaaaaac agggccccca ggtccatcaa cctgcggcaa
     4501 tgctcggtgt cgaacgcggg cgttccccac cacttgccag ccttttcggc cgctttggca
     4561 atcttcttgt aagcgttcca gaccttgggg tgatcccact gcccggggat gccgctgagg
     4621 gtagtgaagt cggcggggcc caggaagaga atatccacgc cgggcaccgc ggcaatctcg
     4681 tcggcccggt cgactgccgc ctgctcttcg agctggatga tgatgaaggt ctcctcattg
     4741 gcggccttca tgtagtcagc catgggcacc gagcaatacg gcatgtcgcc gttgccgccg
     4801 tcgaaccctc tcttgccgat aggatagaac ttggtccact tgaccagctc agcggcttcg
     4861 gctgcgtcgt cgcatcgcgg gtacatcagg ccatgtgctc cggcttccag cagccgggca
     4921 aaacgcatgt attcgccctt ggccgcccgg gcgaccacgt cgcacttggc gacccgggtc
     4981 gcgcggatca gcccgttggc cgtctccacg ctgtagttgt gatgctcgag atccatccag
     5041 atgccgtcca tgcccagcag agcgaccagt tcgaaaacgg attggtcggt gaaatggcat
     5101 tggagacaca aggcgggctg tttccggagc agttttgact tgactgtgct tcgtctcatg
     5161 attcctcggt ccatctcagg ggataaggca agttccccag ttctggcact acacagagat
     5221 ccgataaaac cggcttcgca gggccgtgcg gcgcgtttgt gcatcaacga cccgaggaca
     5281 atcctgcatc acactgactg ccgtaagtca cgcggggaca aaaacgaaca gcaaatctgt
     5341 gcaatcccgt tgttgacgaa accggggggt ttctgtaaac tctaatggca gcgaacacta
     5401 acatagggag tcgtacgatg tcaatagccc ccccgcaaaa tggttcactt cgtgaccagg
     5461 cttactatcg tctgcgccgt ctgctgatac tgcaacaggt gcgtgaggga gagcggttgc
     5521 gagagtcaca ctgggcggcg aagttgtccg tgaaccgcac ggccctgcga gaggcatttg
     5581 cccgattgga agccgaaggt ttcgtgacca aggggccgaa gacgggttac ttcgtcccgg
     5641 ccctgacgaa cgaagacatt cgcgagattc ttgatgtccg cctgatcctg gaaggcggag
     5701 ccattgaacg gatcatcgcc gcgaagaaca acaccccgac gcatctgagg gccatgcgcg
     5761 tcgcctgcga gcagctcgag cgtctggtca gggaggaata catgctggga gtggccgagg
     5821 cagatcgccg gtatcacgaa gcgctgatcg aggcggcggg taacaagcgg ctcgtgacgc
     5881 tttaccaacg ggctcctctg ccgattatcc acccggaagt gatcagcggt cacttgtggg
     5941 aggttcgcgt tcagaagacg ctgaacgagc atcgtgcgat acttgacgcc atcctaaagg
     6001 gcagcgctga agaggcgaag gcgatgctgg gcacgcacct gaccgagaga gccatcgttc
     6061 ctcttcacgc gggatgaccc gcgatcgaag tccgattgtc caaccgatcc tgtcgcatct
     6121 gtccggaggc cgacgtgcca caaaccgccc cgccttgccg aaagacgccg cttatcggat
     6181 gcgtgatgat cattgggggg ttggtcgtcg ccgacgcagc ctgggggcaa accgcggcac
     6241 cggcgtcctc gtccgctgcc gtgccatacg gactgacgac cctggactgg ggcatcatcg
     6301 ccgcatacgg cgcgggcatg ttgggcatcg gggtttacta tgcccgccga acgcgcaacc
     6361 gggaggacta tctgctcggc gggcgcagga tgcgatcgtc gtcggtcggc ttgtcgcttt
     6421 tcgcgacgct gttcagcacc atcacctatc ttgcgctgcc gggcgagatg atcagcaagg
     6481 gaccggtcat tttgtggtcg atgatggcca tcccgatcgc ttacgttctt gtcggttact
     6541 tcattatccc gcgattcatg gagcagcggg tcaccagcgc ttacgagatt ctcgagacgc
     6601 gcctgggtgt ggggctgcgt cttcttgcgg cgatcgtgtt cctggtcacc cgcactctct
     6661 ggatggccct cattatctac gtgaccgccg agaagatcat cgtcacgatg atggacctgc
     6721 ctccggacac ggcggcgtgg gtgacgccgg ttgtcgcggc cgccgtgggc ctgctgaccg
     6781 tggcgtatac cagcatgggc ggcctgcggg cggtcgtttt gaccgacgtc atacaatcca
     6841 tcattttgct cggcggcgcc tgtctgacga ttattctgat caccgtcaag atgggaggcg
     6901 tcacggcctg gtggccaagt gcctggtcac ccaactggga tcaccagccg ttcttcagct
     6961 tcgatccaac cgtgagggtg accgtgatcg gttcaatcct gttcatgacc atctggtgga
     7021 tcggaacggc cggttcagac cagatggcca ttcagcgcta cctcgccacg cgcgacacga
     7081 agacggcccg gcgggtcttt ctggtgaccg gactcgccaa cattctgatc accacgttgc
     7141 tggcctgcgt aggttttgcc ttgctggcct tctttaccgc ccacccggac tatctttccg
     7201 cgggggcgct ggagacgaag gcggacatcg tttttccgct ctacattatc catcatgtgc
     7261 cgccaggcct gaccggtctg atcctggccg ctctgctggc cgcggcgatg tccagcctgt
     7321 cttcgggcgt caattcggcc tgttctgtca tcagcgtcga cttcatcgat cggttccgca
     7381 aggttcccgc cgccgattcc gacgccgtgc aggtgaaacg gattcggatc atttcgttcg
     7441 tgagcggatt tgtggccgtg gtcatcagcc ttctgatggg ccagatcaag ggcatgaata
     7501 tcatggagat cacggtgcgc acgaaccacg tcttcgtcgc gccgctgttc atgctctttg
     7561 tcctggcgtt gttcgtgcgg ttcgccacac cgatcggggc ggcctgcggc acgctggcgg
     7621 gatgcgctgt ggcggcagtg tttgcctgca ccaacaaggt cagtttccaa tggatgagcc
     7681 tggcggcact gctggtcgac ctgctggtag ccgttcctgt cagttactgg acctacaagg
     7741 aaaagcctcc tgcatcgggc gtttcggccg tctcgcggga cggcgtgcgg caatgaacgg
     7801 gcaaatggca ggctcttgtc gaggaaacca tgggtgtctt atgaatcgat tgattgtcgg
     7861 gctcgtctgc ctggtgacgc cctgttgcgt ggtcgggcgc accccgaacg taatcgtttt
     7921 tctcgccgac gacatgg
//
LOCUS       DAOTPW010000263         7900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8148_length_7900_cov_4.776418, whole genome shotgun sequence.
ACCESSION   DAOTPW010000263 DAOTPW010000000
VERSION     DAOTPW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8148_length_7900_cov_4.776418"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..698)
                     /locus_tag="P5316_18485"
     CDS             complement(126..698)
                     /locus_tag="P5316_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87002.1"
                     /translation="MLLRLFTIGFTWAMTAGLAGCGPAAQVRLLQPQYAGAEQDICLQ
                     TNLVRWAPGDGVARFLAEFPLPGAVSGRPTYLLYLRVPMDLPPQTQPAGAAQGVCGFL
                     IQTQGRNAGLEFLKAAQVSMKGKGSAPDARRELRVELIFEGSTTLTGWLKATRDDRYL
                     KIFETRRRPSDVQALLEDSRRGDSVFREVP"
     gene            complement(795..3947)
                     /locus_tag="P5316_18490"
     CDS             complement(795..3947)
                     /locus_tag="P5316_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87003.1"
                     /translation="MTQCQPGDPFDAAPYAFRDAEPDGKAIGLRWGEPRKLRRVVTDF
                     GEKAPPPEKVKLQYWHNTWDGRPDPIRAETGAGGAGWDRMDDWTNGQWRDADAHLDVK
                     GGLWTFTFNPTGNKEYTGMGRPGVGYRKTLKVRLLSEERLPASMEIRAITDAVCQPVR
                     VRVMFGKPADPRISVDKDDAGTIEVFNGAVTSVGALGAARVEPAHTWALRGGAEGSLE
                     FGLMMAVDPVDPRYDRTIVTLRSGNRPFSFAADEVARGHRILVDDLGVLVVRADDGIT
                     LEGYRRARKEFPGRTVYDRVFDEPEQTWQRAWNDMPLKRPLYFVHGLPGNRNAMHQLP
                     DGSLRITGRRQWFNVPKSPKDTDRKLWDGEILHVDFGLPQGGFEAKRELKEGYLPLLR
                     TWWQDGDVHYEQSAILDKLNGGLETIALDDPTVLLVRIRMVNTGKDGTGIARLLFGTH
                     GGDAPETLILRGDQLFAKSGDKERFRFLIISGDKGIASQEGPRIRWKMELRPGQAHEI
                     YCLVPSVTLTEEAEIAALRGRDFDADSKRICEYWRAITARGAQIETPEPAINDFYKSH
                     VRHLLVNCYKEVGSDRLHAHVGTFSYGVYPDESCMMISDLDRRGYHDEARRCLDSFLE
                     YQGTVQMPGNFKSKEGLFYGSGGHDTGGYNKSHGWVMWNMAEHWKNTRDRVWMEKAAP
                     KLIASCDWVTRERRATMRTNADGSRPIEYGWLPAGSLEDVTDYWYWMVTNACTVWGFD
                     ALAAALADFGHPEAARLQADAKAYHEDFMRAMEEARIRCPVVQLRDGTYVPKYPSRLY
                     ERGRCHGWLRETLEGSIHLLITGLIEPHSPQAGWILKDFEDNLYISDTYGYAIPVFNS
                     FWFSRGGFSMQANLLGGPLPYLYRDEIKHFVRTYFNSLASAYYPEIRMCNEHSLPELG
                     YPAGDHFKSSDEAQSTYWLRLMFVHEDGEDLILGQAIPRYWSADGKSVGMERAPSYFG
                     PLSFRLSSEASQGRIRAVVDPPTRNRPRNIYLRFRHPDGKPIRTVSVNGLPYDKIDVA
                     KEWVIIPGSVEGRQEIVAAY"
     gene            complement(4174..4668)
                     /locus_tag="P5316_18495"
     CDS             complement(4174..4668)
                     /locus_tag="P5316_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRR87004.1"
                     /translation="MRGKAAESFEELHVYQRARELTNAVYALTRGPLFSRDRGLVDQI
                     RRASVSIMSNIAEGFERGTTTEFVQYLYVAKGSSGEVRAQLQIALDQGYLQAVEHERL
                     HDLCRRTSGMISNFIAHLQASDYSGEKHARPKRRAIELVQQQQQALRAAQLAGMRPAD
                     RPPG"
     gene            complement(4665..4901)
                     /locus_tag="P5316_18500"
     CDS             complement(4665..4901)
                     /locus_tag="P5316_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87005.1"
                     /translation="MKGMKGEKGGKGVKGGKGVKGGKGVKGEKGVKGEVGIDGRRLTD
                     EGLPAAAGFHRRRRIPQKVPDFIDAVGFRRKGPS"
     gene            complement(5093..6286)
                     /locus_tag="P5316_18505"
     CDS             complement(5093..6286)
                     /locus_tag="P5316_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRR87006.1"
                     /translation="MRSYRFVVLGALAVLVAIGLLFLFVPTAPPTPRVLSAPTSVVFL
                     IGDGMGFEQVKAASLFLNGKEGALSFESWSHQAEVSTYSASSEVTDSAASATAMATGH
                     KVNNGVISQAIPGDGRPLETILERFAAMGRSTGLVTTTEITHATPACFAAHTMDRGKN
                     PDIAECYLNQTRPNVLFGGGGTLKGDAVLAAGYTVVTDRPGLEELDTESVTHVAGLFG
                     EGHMPYEYDYFTKKHLEYDRLPRLSEMSRVALRILSKNPQGFFLMIEGGKIDHAGHGN
                     DLERNVYETIEFARTAEVVMEHIGERRDTLVIVTADHETGGLKVVCGNGKGTLPTVTW
                     STKGHTPVHVPAYAWGVNAHRVTGIMDNTQWFQICLGLAPLPAPTTMPAVAERELSAT
                     FAATR"
     gene            6613..>7900
                     /locus_tag="P5316_18510"
     CDS             6613..>7900
                     /locus_tag="P5316_18510"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRR87007.1"
                     /translation="MNAIIISIGSELTFGQTVDTNSPWLSRRLAEVGVRVIAHVTVAD
                     EMEPIRREIARACELADIVLISGGLGPTEDDLTRQALAAVMGVELRLRPEFLDEIRDF
                     FTRRNREMPESNRIQAMFPAGSEPIHNTCGTAPGIRASIGKATVFAMPGVPREMQVMY
                     ERDVLPHLRAATGQGVLLARTILTFGVGESDVGDKIRDLMQRGRNPTVGTTAQQLIIG
                     IRIHAHGETREAAQSLLDATAADVRARFGTFVFGEDNDTLWSAVVRLLIEQGKTVSTA
                     ESCTGGLIAKSLTDVAGSSACFIEGVVTYSNAAKTRLLGVPADVIARHGAVSRPVAEA
                     MAVNCRRLSGSDYAISVTGIAGPAGGTPDKPVGLVYIGLADASGCEVTEHRIGDFLTR
                     EEIRDRTRKIALNRLRLRLLAQVEHFRGKNGLGDSAR"
BASE COUNT         1523 a         2584 c         2324 g         1469 t
ORIGIN      
        1 agagtattca aaggtggcgg ctctatgtca agctggacac ggaagagcgt tctcgcgaaa
       61 gcgcgatagt tcaagcggct ctttctcaac cgcgacggtg aaaagccgcc ccctgaggaa
      121 gaccgtcagg gaacttcccg aaaaacagag tcgcctcgcc gggaatcctc caggagggct
      181 tggacatcgg aaggccgccg acgggtttcg aagatcttca aataacgatc gtcccgcgtg
      241 gccttgagcc aacctgtcaa tgtggtgctc ccctcaaaga taagctcgac ccgaagttcc
      301 cggcgtgcat cgggagccga acccttgcct ttcatgctga cctgagccgc cttcagaaac
      361 tccaaaccgg catttcgccc ctgggtctgg atcaggaacc cgcaaacccc ctgcgcggct
      421 ccggccggct gcgtctgagg cggaaggtcc atgggcacgc gcaagtaaag caggtacgtc
      481 ggacgcccgg agaccgcccc gggtagcgga aactcggcta agaaacgagc caccccatcc
      541 ccaggggccc agcggacaag gttggtctgc aggcaaatgt cctgctcggc cccagcatat
      601 tgcggctgga gaagccgcac ctgcgccgcc gggccacaac ccgccaaccc cgcggtcatc
      661 gcccatgtga atccgatcgt gaacaatcga agcaacatgg gtactcctcc gaagtcgcgg
      721 ggttcctcgc cccaacctcg accctcagcc gggcgcccgg ccgacgctgt gacccgctgg
      781 aatccgtgcc gacgtcaata ggccgcaaca atctcctgcc gaccttcaac agacccggga
      841 atgatcaccc attccttggc aacgtcgatc ttgtcgtagg gcaagccatt aacgctgacc
      901 gtcctgattg gtttaccgtc cggatggcgg aatcgcaagt agatattcct cggccggttg
      961 cgcgtcggag gatcgaccac cgccctgatc cggccttgcg aggcttcgga gctcaggcgg
     1021 aacgatagcg gcccgaagta agacggagcc ctttccatcc ccacgctctt gccgtcggcc
     1081 gaccagtacc gggggatggc ctggcccaga atcaggtcct cgccgtcttc atgaacaaac
     1141 atgagacgca accagtaggt cgactgggct tcatcagagg acttgaagtg gtcgccggcc
     1201 gggtagccca gctcgggcag cgagtgctcg ttgcacatcc ggatctccgg gtaatacgcc
     1261 gaagcaaggc tgttgaagta cgtccgtaca aaatgcttga tctcgtcccg atatagatag
     1321 ggcaacgggc cgccgagcag attggcctgc atagaaaagc cgccccgcga gaaccagaag
     1381 ctgttgaaaa ccggaatcgc ataaccatag gtatccgaga tatagaggtt gtcttcgaaa
     1441 tctttgagga tccagcccgc ctgtggcgaa tgcggctcta tcagtccggt aatcagcaag
     1501 tggatcgagc cttcgagcgt ctccctcaac cagccgtgac atctgccgcg ctcgtacagc
     1561 cgggacgggt acttcggaac gtacgtcccg tctcgcaact gcactaccgg gcagcggatc
     1621 cgggcctcct ccatcgcccg catgaagtcc tcgtgatagg ccttcgcatc cgcctggagt
     1681 cgggcagcct ccggatgacc gaaatcggcc agggcggccg ccagggcatc gaacccccag
     1741 accgtgcagg cgttggtgac catccagtac cagtagtcgg tcacatcctc gagcgagccg
     1801 gcaggcagcc agccgtactc gatgggcctg gaaccgtcgg catttgttct catcgtcgcc
     1861 cgccgttcgc gagtgaccca gtcgcatgag gcaatcagtt tgggagcggc tttctccatc
     1921 cagacccgat cgcgggtgtt tttccagtgc tcggccatgt tccacatgac ccagccgtgg
     1981 ctcttgttat agccgccggt gtcgtgaccg ccggacccgt aaaaaaggcc ctccttggac
     2041 ttgaagttgc ccggcatctg caccgtcccc tggtattcca ggaacgaatc aaggcaccgc
     2101 cgggcctcgt catggtaacc acgacggtcc agatcgctga tcatcatgca cgactcgtcc
     2161 gggtagacgc cgtatgaaaa ggtccccaca tgggcgtgga gccggtccga gccaacttcc
     2221 ttgtagcagt tgacgagcag gtggcggacg tgggacttgt agaaatcatt gatggccggc
     2281 tcgggtgtct cgatctgcgc gcctcgggcc gtgattgccc gccagtactc gcagatccgt
     2341 ttgctgtcgg catcaaagtc ccttccgcgc aacgccgcaa tctctgcctc ttctgtcagc
     2401 gtcaccgatg gcacgaggca gtagatctcg tgcgcctgac cgggcctcag ttccatcttc
     2461 caacggatgc gcggtccttc ttgcgaagca atacctttat caccgctgat tatgagaaac
     2521 cgaaaacgct ctttgtcgcc gctcttggca aatagttggt cacccctcag tatcagcgtc
     2581 tcgggcgcgt cgccgccgtg cgtaccaaac aacaggcggg caatgccagt gccgtctttg
     2641 ccggtgttta ccatccggat gcgaaccagc aacaccgtcg gatcatcgag ggcaatcgtc
     2701 tccaggccgc cgttcagctt gtccagaatg gcgctctgtt catagtgaac gtcgccgtcc
     2761 tgccaccagg ttctcagcag gggcagataa ccctccttga gctcccgctt ggcctcaaaa
     2821 ccgccttgcg gcagaccgaa gtccacatgg agtatctctc cgtcccacag cttgcgatcc
     2881 gtgtccttcg gactcttcgg cacattgaac cactgccgac gcccggtgat ccgcaggctg
     2941 ccgtcgggca actggtgcat ggcgttgcgg ttgccgggca ggccgtgcac gaagtacagc
     3001 ggacgcttga ggggcatatc gttccaggca cgttgccagg tctgctcagg ttcgtcgaaa
     3061 acgcgatcat agaccgtacg gccgggaaac tccttgcggg cccggcggta gccctccaag
     3121 gtgattccgt catcggccct caccacgagc acgcccagat cgtcaaccag gattctgtgg
     3181 cctcgggcga cctcatcggc ggcaaaagaa aaaggacggt tgccggatcg gagcgtgaca
     3241 attgtgcggt cataacgtgg atcaaccggg tcgacggcca tcataagacc gaattcaaga
     3301 ctgccttcgg cgccgccccg aagggcccag gtatgagccg gttcaaccct ggccgcaccc
     3361 agagcaccaa cggaggtcac tgctccattg aaaacctcga tcgtcccggc atcatccttg
     3421 tcgacgctga tcctcggatc ggcgggcttg ccgaacatca cccggaccct caccggctga
     3481 caaaccgcat ccgtaatagc ccggatttcc atcgaggccg ggagcctctc ttcgctcaat
     3541 aaccgcacct tcagtgtctt gcggtagccc acgccgggcc ggcccatacc ggtgtattcc
     3601 ttgttaccgg tcgggttgaa agtgaaggtc cacaaaccac ccttcacatc caggtgcgcg
     3661 tcggcatcgc gccactggcc gttggtccag tcgtccatgc ggtcccaacc ggcgccgccg
     3721 gcaccggtct ccgcacggat cgggtcaggt ctgccgtccc aggtgttgtg ccaatactgg
     3781 agcttgacct tctcgggcgg gggagccttc tcaccgaagt ccgttacgac gcgccgcagc
     3841 ttccgcggtt cgccccagcg aagaccgatc gccttgccgt cgggttcggc atccctgaac
     3901 gcgtacggcg cggcatcaaa cggatcgcct ggttggcact gcgtcatctg caccgctcca
     3961 tgagataggc ccgttccaca caccagaaga atccacagac aaaccgccgt cggtatcaac
     4021 cggtccgctt cacgcctgca cggcataagc atgcctcctt gtggtgtagc ctgttttatg
     4081 ctccagatcc aagagcgcgg agagtatacc ccccagttgc ataagaatcc acatgcagac
     4141 gcgcagagaa gcatgttgtt catgcgcgat gttttatccc ggcgggcgat cggccggacg
     4201 catgccggcg agctgcgcag ccctcagggc ttgctgctgt tgctgcacca actcgattgc
     4261 ccggcgcttg gggcgggcgt gtttttcgcc tgaatagtcc gaggcctgaa gatgagcgat
     4321 gaagttggaa atcattccgc tggtgcgccg gcagagatcg tgcaatcgct cgtgctcgac
     4381 ggcctgcagg tacccctggt ccagcgcgat ctggagctgc gcccggactt ctccggacga
     4441 gcccttggca acatacagat actgaacgaa ttcggtggtt gtgccgcgct cgaaaccctc
     4501 ggcgatgttg gacatgatcg acaccgacgc acgccggatc tggtcgacca ggccgcgatc
     4561 acgagaaaac aacggcccgc gtgtgagggc atacacggcg ttggtcaact ccctggcccg
     4621 ctggtatacg tgcagctcct caaacgactc ggccgcttta cctctcatga tggtcccttt
     4681 ctgcgaaatc cgacggcgtc tatgaaatcc ggcaccttct gcggaatccg acggcgtctg
     4741 tgaaagccgg cggcggcagg aaggccctca tccgtcagac gcctgccatc aatcccgact
     4801 tcccccttca cacccttctc acccttcaca cccttcccac ccttcacacc cttcccaccc
     4861 ttcacaccct tcccaccctt ctcacccttc atacccttca cacagcattc acccttctcc
     4921 cccgcatcac ccctgcagcc attctctccc gctcaaactc gcagcgttct ctgcttcggg
     4981 tccttgcgtc ccctgcactc tgcctgcttg cccgacgggc gatggtactc ttcgcgtgtt
     5041 gctgatcaat tggcaaacta tcgagtttgc gccacacagg ctgctggagc cgttatcgag
     5101 tcgccgcaaa tgtcgcggac agctctcttt cggcgaccgc gggcatcgtg gtcggagcag
     5161 gcaagggggc caagcccagg cagatctgga accactgcgt gttgtccatg atcccggtca
     5221 cgcggtgagc gttgacgccc caggcatagg ccggtacgtg taccggcgtg tggcctttgg
     5281 tcgaccatgt gacagtgggg agtgtgcctt tgccgttgcc gcagacgacc ttcaggccgc
     5341 ccgtctcgtg atcggcggta acgataacca gcgtatcgcg acgttcgccg atgtgctcca
     5401 tcaccacctc agccgtgcgg gcgaactcga tggtctcgta cacgttccgt tcgaggtcgt
     5461 tgccgtgacc ggcgtgatcg atcttcccgc cctcgatcat caggaagaag ccctgcgggt
     5521 tcttgctcag aatccgcagc gcgacgcggg acatttctga gaggcgtggg aggcggtcgt
     5581 attcgaggtg tttcttggtg aagtagtcgt actcgtaagg catgtggcct tcgccgaaca
     5641 agccggccac atgtgtcacg gactccgtgt ccaactcttc caggcccggc cgatcagtca
     5701 cgacggtgta accggccgcc aggacggcat cgcctttaag cgtcccgccg ccgccaaaaa
     5761 gtacattggg ccgcgtctgg ttgagatagc attccgcaat atcaggattc ttgccgcggt
     5821 ccatcgtgtg agccgcaaaa caggccggcg tggcatgagt gatctcagta gtggtcacca
     5881 gcccggtgct gcggcccatg gcggcgaaac gctccaggat cgtctccagc gggcggccat
     5941 caccgggaat tgcctggctg atcacgccat tgttgacctt gtggccggtg gccatggctg
     6001 tggccgaagc ggccgagtcg gtaacctcgg aacttgctga ataggtggag acctcggcct
     6061 gatgggacca ggactcgaac gacagcgcgc cctccttgcc gttgagaaac aagctggccg
     6121 ccttcacctg ctcgaaaccc atgccgtcgc cgatcaggaa aacaacgctc gtcggtgcgg
     6181 acaagacacg cggcgtgggc ggtgccgtcg ggacgaacag gaaaagcagt ccaatagcaa
     6241 caaggacggc cagtgctccg agaaccacaa acctgtaaga cctcatgcgc atcatctccg
     6301 cgagcaacga ataacccggc acggccacct cggcgcgcgg gcaccccctg ggaggctgtc
     6361 agacaggttg attctagccg cgcgcggtcg cacaatcacg cgcgatcagg gtgcgcagtc
     6421 cggattggcg gggacgcccg aaccgctcag gcagccgagg aagacgctca gctcagcctg
     6481 atcgacgtcg ctgtcaccat ggccattgaa gattggtacc cccttcaaca tgaccgactg
     6541 ccctctgtcc agcagtagag ccactggtgc gcgcccttca acccgactgc aacccccggt
     6601 atggtttgcg tcatgaacgc catcatcatc agcatcggca gcgagttaac tttcggccag
     6661 accgtcgaca ccaacagccc ctggctgtcc cggcggctgg cggaggtggg ggtacgcgtg
     6721 atcgcccacg tcaccgtggc cgacgagatg gagcccatcc ggcgggaaat cgctcgggcg
     6781 tgcgagctgg ccgacatcgt gctgatcagc ggcgggctgg gaccgaccga agacgacctg
     6841 acgcgccagg ccttggcggc ggtgatgggg gtcgaactgc ggctgcggcc ggagttcctg
     6901 gacgagatcc gcgacttctt cacccgtcga aaccgcgaga tgccggagtc gaaccggatc
     6961 caggcgatgt tcccggccgg cagcgaaccg atccacaaca cctgcggaac cgctccggga
     7021 atccgggcgt cgatcggcaa ggcgacagtt ttcgcgatgc ccggcgtgcc ccgcgagatg
     7081 caggtcatgt acgagcgcga cgtcttgccg cacctgcggg ccgccaccgg tcagggcgtc
     7141 ctgctggcca ggacaatcct gaccttcggg gtgggcgagt cagacgtcgg cgacaagatt
     7201 cgtgacctga tgcagcgggg tcgcaacccg accgtgggaa cgaccgccca gcagctcatc
     7261 atcggcatcc gtatccatgc acacggcgag acacgcgagg cggctcagtc gctgcttgat
     7321 gcgaccgcgg cggacgtccg cgcgcgattc ggcacattcg tgttcggcga agacaacgac
     7381 acgctatgga gcgctgtcgt tcggctgctg atcgaacagg gcaaaaccgt ctcgacggcc
     7441 gagtcgtgta ccggcgggct gatcgccaag agcctgaccg acgtggcagg cagcagcgcg
     7501 tgcttcatcg aaggtgtggt cacttactca aatgccgcca agacgcggct gctgggtgtg
     7561 cccgcagacg taattgcacg tcacggagcc gtgagccggc cggttgctga agcgatggcc
     7621 gtcaactgcc gccggctgag cggctccgac tacgccatca gcgtcaccgg catcgccggc
     7681 cccgccggtg gcacccccga caaacccgtc gggcttgtct acatcggcct ggccgacgcg
     7741 tccggctgcg aagtgaccga gcatcgcatc ggtgatttcc tcacccgcga ggaaatccgc
     7801 gaccgcaccc ggaagatcgc cctcaatcgc ctgaggctgc ggctgttagc ccaagttgaa
     7861 cactttcgag gcaagaatgg ccttggcgat agcgccaggg
//
LOCUS       DAOTPW010000264         7892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8156_length_7892_cov_5.536940, whole genome shotgun sequence.
ACCESSION   DAOTPW010000264 DAOTPW010000000
VERSION     DAOTPW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8156_length_7892_cov_5.536940"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3947)
                     /locus_tag="P5316_18515"
     CDS             complement(<1..3947)
                     /locus_tag="P5316_18515"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRR87008.1"
                     /translation="MEPRASQDLYWMVLVAALAAMPVAGGITGVQAAEVIGQPIRINA
                     TGTSGGNTSAESGPVIQVSEEAASLMARAREGVDRRDWKLAIDSLQRIVELPGEHILR
                     VNDNRYEMARIQAHRQIAALPAEGLEAYRTLNDAEAAMLLEEGRREHDAELLATVVHR
                     YLVTRSGDDAAVTLAEWLMDEGRFFEAASLLRTVRLIYPDSDLPGWLVPARLTMCFAA
                     MGQQRRAEEMLAQLTATEGASSLPQKKAQQLRELVSKSNNRPEPQRATSWPMACGNVC
                     RGGVQHVVEPSLVEQHCMTAMLPIPAPAEVAATLEEVAVKAGFIPGRHIVTHEDLVIV
                     KAARNLVALDANTLTPVWTTKPPIDAVESPAEDESFQIWQIAEPSATGSQDLRQPQAL
                     LALACAANSDVVIVEDKVLTLVFPEGALPAPNLQRQQNVNVIFWPDSVAVSTRPNHVQ
                     AYALKDGSFLWQSDTSGPAGTAMAEEVAWQFLATPVPAEGHLLAPCKMNNDLYLVALD
                     PATGKHAWHVFLCGMSATTSFSAMESMRIAISDGVAFVLTGQGLLVAVELSGRSVLWA
                     IRYPRRETTDRYPTGKIIRGDEASRAWEPPVATADVLLAAPVDANHLFCIDRFTGDVR
                     WQAELHEAVQILGVDHRYVWLAGNQLQMLDLHTGLPVWTKTSIKLNGRGSLSGDRIYL
                     PTTGGLAAFEAKTGEPVPLKGTGGVMPGNLLAWDGALYSVGISEVRKYPDLESGYKTA
                     VARHQADPADPSKALRLAGLELLLNNPAAALQALVLLGDDLKTTDPWKHGQAVHLRVE
                     ALLALAADEKRTEAEAEESLKQARAIAESAEDRIRTALAMGDHLRLRQRFLEACGQYL
                     LLALSPAGDEMVSLEKGLEQEARLLANQRLAGVISQLSAEDRKTLAHRARQQLSEGLN
                     NRDADSLRRLMDCPAAADASYDARLHLAIWAIENLQFEQAESLLRQIVNHGASPETRA
                     EAAARLAAICLLPGELHQPVTGAALLDRLQTEWDGVEIPADVLADPLEPTTERPASSS
                     KVTGRVAADTLRRRLEASSLARSGAVAGPCVLGPLQGGVSLLTENNRPLIARNDSSEA
                     AQTTLVSMTENKVLKARRLGIRRALWQTELRLSEQPAVDVGTGISVTSFQTQIGEASG
                     TRWVVRPAVGVLDGQTLIVNSGFGLHGVGLLTGRRLWSRPFDPPCAPGSDPIASDLWL
                     WSHDGYLISLNASGRVEVARSFDGSDVLWSRSMPKRQWHIVRARGSYVIAADEKIQEV
                     DVFRLADGQHIGQCRFSQPEAAADRINLLLFDEVICGPVSENEVAAFELASPGT"
     gene            4330..4899
                     /locus_tag="P5316_18520"
     CDS             4330..4899
                     /locus_tag="P5316_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87009.1"
                     /translation="MRSTTMEWNMMSRTSCVAVALAVLALAGCTCVDGPPADQMNAPP
                     QGTAPTGSRLAENYVAMTDNALLNDASMSSVHFVPRTAELNALGVRRLTRMAEVLKVY
                     GGTVFYDGSDAERDLRKDRVEKIRLYLTSCGIDPSRFKVEEGFAGGSGIDGDEAVAIR
                     RATRGPGDVKIQAQAAGSGSSGGQGGGSQ"
     gene            5010..6782
                     /locus_tag="P5316_18525"
     CDS             5010..6782
                     /locus_tag="P5316_18525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR87010.1"
                     /translation="MTKMKRFSLIARQTAFVSVLLVGCGSSDSTREKLMNVPGNSAQM
                     PSWEATKQSETGMPDIAPETYVAAGRMHESQGRLTRAAAQYRMAIQLDPRHVEAHNRL
                     GLVLCQMQQFKEADAVLAKAVELAPGRPHLHNNQGFSFLVQMRWTEAEQSFRKALELQ
                     PDFARAHMNLGMVLAQQDRFDEAMRHFLAVVPVEDAWFNIGLMYQSKSRPAEAARAFK
                     TALKYNAKMAAAQQCLNKLPQEISGSATPFDSLTALAATPKVKPLPEQPQPPVESLIV
                     ATTQPEADGQPPTVQPAFEDIRTAESPDLTFEEPFESPSEMLETAQTVEHGPSTPIQE
                     SSALHQVPPTEALAEVIEPTPDSSPAPQEEKSIHGRPLDPQILAALMNTDPFNINDNL
                     PEIDPLAESPDQENNQVEPATETPAVAERVETTTPPIANTTSQPTSITYDFLGSQAPN
                     DLFAMNWMSKNATDEQFEIVLSVPPPLPPAARNAVLISTQPTTEAPTPEELRTVVEAL
                     LQLSHSPTDAMPKPYSVPTSTRPAAGGPQSQPATGRTPTPRQPDVTAGTNVPDQDEVW
                     RRPLWDDLEAFGPEEPSGSTTPDD"
     gene            complement(6957..>7892)
                     /locus_tag="P5316_18530"
     CDS             complement(6957..>7892)
                     /locus_tag="P5316_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87011.1"
                     /translation="LWNDGWMYKGLTSAVKVLRLAGHPRTEEFAAEARDYQRTFVAAL
                     RAKAAEMPTWTDCKGKTHRMVPAALGGATEVDPRHPFYLDTGPLFLVFSGFVDARDEL
                     AREAIAWFREGPQLAMLREEPLYEQMPGLRHEMSSWEPCYSWNVFHSHAMNERYQFLE
                     GMYSLYAGSVSRETFVSCESRGGITGNVFSAPLAIYLSRLAIIDDQIEPEALHLLRIM
                     PRAWLRTDRETRFEKMPTAFGPVTVKARINREGKMLEVTYRASFRRPPQKVLLHVPPV
                     PGLKAVRVNGKPVAWDGRSGSIELVPSLTGEKVIP"
BASE COUNT         1532 a         2668 c         2250 g         1442 t
ORIGIN      
        1 gtgccggggg aggccagctc gaatgccgca acctcgtttt ccgataccgg cccgcaaatc
       61 acctcatcga atagcagcag gttgatcctg tccgcagccg cctccggttg gctgaatcgg
      121 cactgaccaa tgtgttgacc gtcggccagc cggaacacgt cgacctcctg tatcttctcg
      181 tcggccgcaa tcacgtagga gccccgagcc cggacaatat gccactgccg ctttggcatg
      241 ctccggctcc atagaacgtc ggatccgtca aacgatcggg cgacctcgac ccgcccggag
      301 gcgttgaggc tgatcagata gccgtcatga ctccagagcc acaggtccga ggcaattggg
      361 tccgatcccg gtgcgcaagg gggatcaaat ggtcgtgacc acagccggcg gccggtcagc
      421 aggccaaccc catgaaggcc aaacccactg ttgacgatca gagtctggcc atccaggaca
      481 ccgacggccg gccgaaccac ccagcgggta cccgaggcct ctcctatctg agtctgaaag
      541 gaagttaccg atattcccgt acccacatcc accgccggtt gctcggacaa ccgcagttcc
      601 gtctgccata gcgcccttcg tatgcccaat cgtcgagcct tgagaacctt gttctccgtc
      661 atggacacca gagtcgtttg ggccgcctcg ctggaatcgt ttcgggcgat cagcggacgg
      721 ttgttctcgg tcagcaagct gacgccgccc tgcaaaggcc ccagtacgca aggcccggcc
      781 actgcccctg acctggccaa cgatgacgct tccagccttc gacgcaaggt gtcggccgca
      841 acgcgaccgg tcaccttcga agatgaagct ggacgctccg tcgtcggttc cagcgggtca
      901 gccagtacgt ccgcagggat ctcgactcca tcccattcgg tttgaagccg atccagaagc
      961 gcagccccgg tcaccggctg gtgaagttct cctggcagca ggcaaatcgc ggccagccgc
     1021 gccgccgcct ccgccctcgt ttccggtgaa gcgccatgat tcacaatctg tcgcagcaag
     1081 ctctccgctt gctcgaactg cagattctca attgcccaga tggccagatg tagccttgca
     1141 tcgtaagacg catccgccgc ggcggggcag tccatcagcc gccgcaggct gtctgcgtcg
     1201 cggttgttca atccctcgga cagttgctgc cgagcacggt gcgccaacgt cttgcgatct
     1261 tcggcggata gttgggaaat gacgccggcc agtcgctggt tggcaagcag ccgcgcctct
     1321 tgctcaagcc ccttctcaag agaaaccatc tcatcgccgg ccggcgaaag agccaataac
     1381 agatattgcc cgcatgcctc cagaaaccgc tgacgcagcc tcaaatggtc tcccatggcc
     1441 agagcagtcc ggatgcgatc ttcggccgat tcggcgatcg cgcgggcttg cttaagcgat
     1501 tcttccgcct cggcctcggt cctcttttcg tccgccgcaa gcgccaacaa cgcctccacc
     1561 cgaagatgaa ctgcctgccc gtgcttccac gggtccgtcg tcttcagatc gtcgcccagc
     1621 aaaaccagtg cttgcagggc ggcggccggg ttgttgagca aaagctccag gccggccagc
     1681 cggagggctt ttgatggatc ggccgggtcg gcctgatgcc gggcaacggc cgtcttgtac
     1741 ccactttcca ggtccggata cttgcgcacc tcactgatgc ccacgctgta cagcgcgccg
     1801 tcccaggcaa gcagattgcc cggcatgacg ccgcccgtcc ccttcagcgg gaccggttca
     1861 ccggtcttgg cctcgaaggc cgccaggccg ccggtggtcg gcaggtagat gcgatcgccg
     1921 ctgagcgaac cccgaccgtt caatttaatg cttgtcttgg tccagaccgg caggccggtg
     1981 tgcagatcga gcatctgaag ctgattgccc gccaaccaca cgtaccggtg atctactccg
     2041 agaatctgaa ccgcttcgtg cagctcggcc tgccaccgca cgtctcccgt gaaacggtca
     2101 atacaaaaca gatggttcgc gtccacagga gcggccagaa gcacgtcggc ggttgccacg
     2161 ggcggttccc aggcccgact ggcttcatcg cctcgaatga tcttacctgt tggatatcga
     2221 tccgtggtct cccgtctcgg gtaccgaatg gcccatagca ccgaccgacc tgacagctcg
     2281 acggcgacga gcaaaccttg tcccgtgagc acgaacgcca ctccgtcgga tatggcgatc
     2341 cgcatcgatt ccattgcgct gaacgaagtc gtcgcactca ttccgcagag aaaaacatgc
     2401 cacgcatgct tgccggtggc cggatccaga gccactaggt aaagatcgtt gttcatcttg
     2461 cagggggcaa gcagatggcc ttccgccggg acgggcgtgg ccaggaattg ccacgcaacc
     2521 tcctccgcca tagccgtgcc ggccggcccg gacgtgtcgc tttgccataa gaacgagccg
     2581 tccttgagag catacgcttg aacgtggttg ggccgcgtgc tgaccgcaac agaatcaggc
     2641 cagaaaatga cgttcacgtt ttgttgcctt tgcaggttcg gagccggcaa cgcgccttcc
     2701 ggaaagacca gcgtcaatac cttgtcttcc acgatgacca cgtcgctgtt ggccgcacag
     2761 gccaacgcaa gcaaggcttg aggctgtcgc aggtcttgac tgccggtcgc ggaaggctcg
     2821 gcgatttgcc aaatctgaaa agactcgtct tctgcgggcg actcaacggc gtcgatcggc
     2881 ggcttcgtgg tccacacggg tgtgagcgtg ttggcgtcca gcgcgaccag attgcgggct
     2941 gccttgacga tgacgaggtc ctcatgggtc acgatatgac gcccggggat gaaaccagcc
     3001 ttgaccgcca cctcctccag cgttgcggct acctcggccg gcgcgggaat cggcagcatc
     3061 gccgtcatgc aatgctgctc caccaggctc ggctcgacga catgctggac gccgcctcgg
     3121 caaacgttgc cgcacgccat gggccatgat gtcgcccgct gcggctccgg cctgttattg
     3181 ctcttgctga ccaactcccg gagttgttgc gccttctttt gcgggagcga agaagccccc
     3241 tccgtagccg tcaactgcgc gagcatctcc tccgctcgcc gctgctgccc cattgccgca
     3301 aagcacatgg tcaatcgcgc gggcaccaac cagcccggca ggtccgagtc agggtagatc
     3361 agacgaacgg tccgcagcag cgaggccgcc tcgaagaacc gcccctcgtc catcagccac
     3421 tccgccagcg tcacggccgc atcatcgcct gaacgggtca ccaggtatcg atgaaccacc
     3481 gtcgccagca gctccgcatc gtgctctcgc cgcccctcct cgagcagcat ggccgcctcc
     3541 gcgtcgttca gcgtacggta ggcttccaga ccttctgccg gcagcgccgc gatctgccga
     3601 tgagcttgta tgcgggccat ttcatacctg ttgtcattta cccgcagaat gtgttcgccc
     3661 ggcagctcca cgatccgttg gagagagtcg atcgccagct tccagtcccg gcgatccacg
     3721 ccttcacggg cccgcgccat cagtgacgcc gcttcctcgg aaacctgaat caccgggccg
     3781 ctctcggcgc tcgtgttccc gcccgaggtt cccgtggcgt tgatgcggat cggctgaccg
     3841 atgacctcgg cggcttggac accggtgatg ccgcccgcaa ccggcatcgc cgccaacgcc
     3901 gcgaccagga ccatccagta caggtcttga gaggctctag gttccacctg tggccgatcc
     3961 cttccgttgg gcttggcagt cctgcaaaac gtccgccgca tgcgaccgcg ccgtcctcgt
     4021 tcgccggcgc cgactgctcg gtggatgccc gcccccggaa agtcttatca taacccgtct
     4081 ccgctcttcc gccaactgta ttagcggact tccgtatccg ggatcgccgg ctgggtgcgc
     4141 ctgcgcctcg cgagactctt tattgggcgt tcaatcaacg gctcttccca acccgacgcg
     4201 atcgccccgc tctctccgaa gcctccgaca aatagcaatc tgaggagccc ttttggccga
     4261 tgaaccgcaa tgagttgctt ttgtgtctgt ggtccgaagt ccgaatatgc gccggaaggg
     4321 gaatggaaga tgaggtcaac gacgatggag tggaacatga tgagccggac atcctgtgtg
     4381 gcggttgcct tggccgtgct cgcgctcgcc gggtgcacct gcgtcgacgg cccgcctgct
     4441 gatcaaatga acgcgccgcc ccaggggacg gcaccgacgg gcagccgact cgccgagaac
     4501 tatgtggcga tgaccgacaa cgcgttgctc aacgacgcct cgatgtcgtc cgtccacttt
     4561 gttccccgaa ccgccgaact taacgccctc ggagtacgcc gcctgacccg gatggccgag
     4621 gtcctcaagg tctacggggg aaccgtcttc tacgacggca gcgacgcgga acgcgacctc
     4681 cgaaaagacc gcgtcgagaa gatccgcctg tatctgacaa gctgtggtat tgatccctcg
     4741 cgattcaaag tcgaagaggg attcgccgga gggtccggca tcgacggcga cgaggccgtt
     4801 gccatccgca gggccacgcg gggaccgggc gacgtgaaga tccaggcgca ggccgctggt
     4861 tccggatcga gcggcggcca gggcggcggc tcgcagtgat gggcgccgca gaagcagagc
     4921 tgttggccgg cacaggcacc ggtacccgga atcatccgtt gccgggccca cgccatgatg
     4981 cgggagaaag ctgagcggga gcgttgcgca tgaccaaaat gaagagattc agcctgatag
     5041 ccagacaaac ggcgtttgtg tccgtgctcc tggtcggatg tggatcgtcc gactccacca
     5101 gggaaaagct gatgaacgta cccggcaact ccgcgcagat gccttcgtgg gaagccacaa
     5161 agcagagcga aacgggaatg ccggacatcg caccggaaac ctacgtggcc gccggccgca
     5221 tgcacgagtc gcagggtcgc ctcacacgcg ccgctgccca gtatcgcatg gcgatccaat
     5281 tggatcccag gcacgtcgaa gcacataatc ggctcggact cgtcctctgt cagatgcagc
     5341 agttcaagga ggccgacgcc gtactcgcca aagccgtcga gcttgctccc ggaagaccgc
     5401 accttcacaa caatcagggt ttcagctttc tggtccagat gcgctggacg gaggccgagc
     5461 aaagcttccg aaaagcattg gaactccaac ccgatttcgc ccgcgctcat atgaatctgg
     5521 gcatggtcct tgctcagcag gatcgatttg acgaggcaat gagacacttc ctggccgtcg
     5581 tccccgtcga ggacgcctgg ttcaacatcg gccttatgta tcagtccaag tctcgacccg
     5641 ccgaggccgc ccgagccttc aagaccgccc tgaagtacaa cgccaaaatg gccgccgcac
     5701 agcagtgcct caacaagctt cctcaggaaa tatccggcag cgccacgccg tttgatagct
     5761 tgaccgctct ggccgcgacg ccgaaggtga aaccgcttcc cgagcaaccg cagccgccgg
     5821 ttgagtccct gattgtcgcc actactcaac ccgaggccga cgggcagccg ccgactgtcc
     5881 aaccggcatt tgaagacatt cgtacggccg agtctcccga tctgaccttc gaagaaccct
     5941 tcgagtcacc atcggaaatg ctggaaacgg cgcagacggt cgagcatggg ccgtcgacgc
     6001 ctatccagga atcgtcggct ctccatcaag tgcccccaac agaggcgctc gccgaagtaa
     6061 tcgaaccaac ccccgattcg tcgccggccc cccaagagga aaagtcgatc cacggcaggc
     6121 ccctcgatcc tcagatcctt gccgccctga tgaacaccga cccgttcaat atcaacgata
     6181 acctgccgga gatcgatccc ctcgccgaat cgcctgatca ggagaataac caggtcgagc
     6241 ctgcaactga aacgcccgct gtggccgaac gcgttgagac gacgactccc ccgattgcga
     6301 atacgaccag ccaaccgacg tctatcacct acgatttcct cggcagccaa gccccgaacg
     6361 atctgtttgc catgaactgg atgtccaaga atgccactga tgaacagttc gagatcgttc
     6421 tatcggttcc gcctcctttg cccccggcag cgcggaacgc cgtactgata tcaactcaac
     6481 ctaccactga ggctcccact cctgaagagc tccgcaccgt cgtcgaggcc ttgctgcaat
     6541 tgagccattc gccgaccgac gccatgccga agccgtacag cgtgccgacg agtacccgtc
     6601 ccgccgctgg tgggccgcaa tcccaacccg ccaccggccg gaccccaacc ccccgacaac
     6661 ccgacgtcac ggctgggacg aacgtgcctg atcaggacga agtctggcgc cggcctctgt
     6721 gggacgatct cgaagccttc ggacccgagg agccgtcggg ttcgacaacc cccgacgact
     6781 gatcatgtga acggagcttc ttccgtcagc aaaccagatg cacggcgacg gccgatcctt
     6841 gtgatcggcc gttttggtct cttccgtcgc gacaggcacg cgttttgcgc gcatgttctt
     6901 gggtcacaag acgctttgct tgcgacccga aaaaaccgtc cgcctcagtc cgttgctcac
     6961 ggaatgacct tttcgcccgt caaagacggc accagctcga tactccccga acgaccgtcc
     7021 cacgcaacag gtttgccgtt caccctgacg gccttgagcc cgggcacggg tgggacatga
     7081 agaagaacct tctggggcgg ccggcggaaa gatgcccggt atgtcacttc gagcatcttg
     7141 ccttcccggt tgatccgggc cttcaccgtg accggcccga acgccgtcgg catcttctcg
     7201 aatcgcgtct cgcggtcggt ccgtagccat gcccgcggca tgatccgcag caagtgcagc
     7261 gcttccggtt cgatctggtc gtcgatgatc gccaggcgtg ataaatagat ggccagcggt
     7321 gccgagaaca cgttgccggt gatcccgccc cggctttcgc aggacacgaa cgtctcacgc
     7381 gagaccgacc ccgcataaag gctgtacatg ccttccagaa actgataccg ctcgttcatc
     7441 gcgtgcgagt ggaacacgtt ccaactgtag cacggctccc aactcgacat ctcgtgacgc
     7501 aggccgggca tctgttcata taacggttcc tctcggagca tcgccagttg cggaccctct
     7561 cgaaaccagg cgatcgcttc ccgtgccagc tcgtcgcggg catccacaaa tcccgaaaac
     7621 acaaggaaca gcggaccggt atctaggtag aacgggtgtc gcggatcgac ttcagtcgca
     7681 ccccccaacg cggcagggac catccggtgt gttttgccct tgcaatccgt ccacgtgggc
     7741 atctcggccg ctttggctcg cagagccgcg acaaaagtcc gttgatagtc gcgagcctcg
     7801 gcggcgaatt cctctgtacg cggatgcccc gctaaccgca gcaccttgac cgccgacgtc
     7861 agacccttgt acatccaccc gtcattccat ag
//
LOCUS       DAOTPW010000265         7785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8296_length_7785_cov_6.883959, whole genome shotgun sequence.
ACCESSION   DAOTPW010000265 DAOTPW010000000
VERSION     DAOTPW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8296_length_7785_cov_6.883959"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..954
                     /locus_tag="P5316_18535"
     CDS             151..954
                     /locus_tag="P5316_18535"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRR87012.1"
                     /translation="MLSLPGCAPLWVDSPSTPATFAAGWTIVRVQRANGTHFSALLYY
                     PALATGRDAPVDPTGGPYPAVTFGHGWLVRPRWYDSTLDHLAAQGYVVIASASQTRLF
                     PSHPKFAADMRQCLTYLEQQNVDQDSWLCGLIDTERFGVAGHSMGGGASLLAAAADDR
                     IKAVVALSPAETWPDSAIAAAASIHVPICYIVGSQDSFTPLEVHTQPMYDNSNPPRQL
                     LVLIGGYHCGFIDPAVPLFCDSGTMDHRLQLDITWERMTEFFDLYLKGR"
     gene            complement(941..2380)
                     /locus_tag="P5316_18540"
     CDS             complement(941..2380)
                     /locus_tag="P5316_18540"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR87013.1"
                     /translation="MAAYCFRRVVLSCTFFVSGVSWAWAGSIAGYAHDFDDASLYEME
                     ITQCRSGESTNLAWARISNNGYYEIKDLAAGEYSLVLGDLYYFRPKLFSFVPVREDIV
                     TPGTFQVDAAYFVRVRATEKSPACREIRQTFVATGDVVKVTVWTFDNAALYCSIHDAI
                     THERIGPARDQAVDAADSFTWRHGEVPALPGQSYYVKLWRPDNGEFSLSVCEKKGGRS
                     YGPGHAFYDGRPEPQADIRCVIECDDDGLWTMAQWQRTDGKGHGSTHIGQTFKAIGSF
                     VNVVTLFGTAIEGQYVRTRFSIHEDGPEGRQVGPTKTCHPWNYRPMGQVYSVSWQPGE
                     VPVTSGRTYYLKALADEPGLFAFTNKHDGYPLGRCYFGGTAGPENEDLCFTVLGEAEL
                     HSSLSTLKGTVKTLTNKAIPGATVTLNPWGYSVKSRPNGEYTLSRVTAGRYAVCCSAD
                     GYRPRTFVVTLRPAATHEMDFTLTSLTGP"
     gene            complement(2642..3664)
                     /locus_tag="P5316_18545"
     CDS             complement(2642..3664)
                     /locus_tag="P5316_18545"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRR87014.1"
                     /translation="MGQARGRTLKLLGLIAVIIAMVLLLRLKGEQKKTLRVFAADALA
                     HSFTRIKSEFEKEHPDVNIELEFQGSVVLLRLVPLRKCDVAAVADARLVERMLNPHTA
                     TWVVKFGTTEMVIGHTDAGKYASEITPDNWYEILLRKDVHYSHADPTQDPCGYFAMLC
                     WKLAEKHYATKTGHRRLYEELKAGCDPRHVKEDALTLLSLLETSAAYDYAFLYRCHVV
                     DHHLPFVSLPPAINLGDPGLESQYAKAQTEVPSYRGGVETISGSCVSFGITITEGCRN
                     RTIAQEFVRFVLSEKARAILRDSAIVPLDPPVVPSWGRIPAFLEGTAVAENGPVTTSQ
                     AAMLRR"
     gene            complement(3763..4890)
                     /locus_tag="P5316_18550"
     CDS             complement(3763..4890)
                     /locus_tag="P5316_18550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR87015.1"
                     /translation="MIQVCDVIARYGYFSLKKVSLLISAGECFALIGPSGAGKTLLLE
                     TIMGVKPPAHGRILVEDADITTAPPEQRRFSYVPQDLALFPHLSVPENIGFGLRIRNT
                     PSTEIDRRIRSVASMLRIESLLSRRDVRSLSGGEKQRVALARALAVEPRVLFLDEPFG
                     ALDTSTRRQLHVEMRDVQRRLRLTTVLVTHDLDEAFALADRIGIMIDGGIEQVGQPRF
                     VYENPANLPVARFLLVENILRGQCVGAGPAEGERLFRVGDLMIAAAHGEPITPDRACW
                     LGIRAQDVEITPEVADGPGSCTEEGSPAVIEAVRRHACTCTVLMRSLSDQVRLEGQLS
                     SRREATMRLEPGQRVRIRIPSRRVLIFPGEDAAPVAAGSSG"
     gene            complement(4887..5708)
                     /locus_tag="P5316_18555"
     CDS             complement(4887..5708)
                     /locus_tag="P5316_18555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRR87016.1"
                     /translation="MVVAARRLLGAQRDMIRNRFDAILLTAVGVSTTLMLMLILVQGA
                     YTGAKAPLRALTEHDVQHAIYLSFITSGLASVAAILLAIPTGLALARIQFPGRWLVES
                     LLIVPIVMSPISLGVALLLIFRTEAGTWIEDHLIRFVFEVPGIILAEFFLAYAISVLV
                     VRSTFSAVDARLEQVARFLGCTPWQAFRHVSLPLARNGIIAAFVLGWVRAMGDFGASS
                     TIAGAVKGKTETMPVSIYLNLASVSLDRAIALSLVLTFVTVVVVIMVGLLMRRSS"
     gene            complement(5702..6463)
                     /locus_tag="P5316_18560"
     CDS             complement(5702..6463)
                     /locus_tag="P5316_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRR87017.1"
                     /translation="MTLELFVGSATKPPVEEAAQVYENATGTRMLLHFGGSGKMLAEM
                     KLARRGDLYFPGSSDFMEMAKRERLVLPETERRVAYLIPAINVPAGNPKGIKGLEDLA
                     KPGVRVGIARPDSVCVGLYAAEVLERNGYAARVKPNIVTHVESCEKTAQLVAIGSVDA
                     ILGWEVFHYWQPDKIETILLKPNEVPRIGYLPIAVSVFSKEKEQALKLIEFLVNGQGR
                     ESFRRWHYVTTLEEARTYALPDTPVGGEWRLPEEW"
     gene            complement(6873..>7785)
                     /locus_tag="P5316_18565"
     CDS             complement(6873..>7785)
                     /locus_tag="P5316_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87018.1"
                     /translation="TDVWRVYHVERTQDVIRVLGSHRGSVRELRVSPEGDYIATVGTD
                     GNCRVWNTASSAAAPLILSVPGGGDDRGAAAFLDDGAHIACGGTGVVDIVSLRDGTLV
                     RRIPDRQHDMLYEGSDFQTLGKTLKESRILSLDARPGGRHLLVGTDSGVMEIDYLRCR
                     IMWRQKERVGPIATLVRYLPDGEQFIVQDDSCRLTLYAGHKSVWSIRPGESGPGGRGL
                     VKLTAAEFTNDSAYLAALGSDGSFRVLRLSDQKVVQELAPGKLDTAGLALSLERASAW
                     VGSQGNVERFTRLALPREMTSPKVAID"
BASE COUNT         1527 a         2561 c         2229 g         1468 t
ORIGIN      
        1 atgcaaaaaa caggtgcggt ccatgaataa tgggtgttgt tcgaggtatg accggacagt
       61 cgtgatgttg tataatcaat ccccatggtc agcggcttct cctgcagatt ggcgaggagt
      121 cttgcgtctg ccggcatcgc ttgcagcacg gtgctcagtc tgcctggctg cgcgccgctg
      181 tgggtcgatt cgccctcaac tcccgcgacc tttgcggccg gttggaccat cgtgcgcgtg
      241 cagcgggcca acggcactca cttcagtgcc ctgctctact atccggcgct cgccaccggc
      301 agagatgccc ccgttgaccc gaccggcggc ccatatccgg ccgtgacgtt tggccatggc
      361 tggctggttc gcccgcggtg gtatgactcg accctggacc atctggccgc gcaaggatac
      421 gtggtcatag cctccgcctc gcaaacccgg ttgtttccca gccacccgaa gttcgctgcc
      481 gacatgcgac agtgccttac ctaccttgaa cagcagaacg tcgatcagga ctcgtggctc
      541 tgcgggctga tcgatacgga gcgcttcggc gtcgccggtc attcgatggg agggggagcg
      601 agtctcctgg cggccgccgc cgacgatcgc atcaaggcgg tcgtggccct ctcgccggcg
      661 gaaacctggc ccgactcggc catcgccgcc gcagcgagca tccacgttcc gatctgctac
      721 atcgtcggct cgcaggatag cttcacgccg ctcgaggttc acactcagcc aatgtacgac
      781 aattccaatc caccccggca actccttgtt ctcatcggcg gctatcactg cggctttatt
      841 gatcccgcgg taccgctgtt ctgcgattcc ggcaccatgg accatcggct tcaactcgac
      901 atcacctggg agcggatgac cgagttcttc gacctttatc tcaagggccg gtaagagatg
      961 tcagtgtgaa gtccatctcg tgagtcgctg ccggtctgag ggtcacgaca aaggtcctcg
     1021 gccgataacc gtcggccgag cagcagaccg catatcggcc cgcggtcact ctcgacaagg
     1081 tgtattcgcc gttcgggcgg ctcttcaccg agtaacccca gggattcaat gtgaccgtcg
     1141 cgcccgggat cgccttgttt gtcagggtct tgaccgtgcc cttgagtgtg ctcagcgaac
     1201 tgtgcaactc agcctcaccc aaaacggtga agcagagatc ctcattctcc ggccccgcag
     1261 tgccgccgaa gtagcaacgg cccagcggat agccgtcgtg tttgttcgta aacgcaaaga
     1321 gtccgggctc gtcggcaagg gctttcaaat agtatgttcg gccggaggtg accggtactt
     1381 cgcccggctg ccagcttacc gagtagacct gtcccatcgg gcgataattc cagggatggc
     1441 atgttttggt cgggccaacc tgcctgccct ctgggccgtc ctcgtgaatg ctgaatcgtg
     1501 tcctcacgta ttggccctcg atcgcggtgc cgaacagtgt caccacgttg acgaaggacc
     1561 cgatcgcctt gaaagtctgc ccgatgtgag tgctgccgtg gcctttgccg tcggttcttt
     1621 gccattgagc catcgtccac aacccgtcgt cgtcgcattc gatgacacag cggatgtccg
     1681 cttgcggctc cggacggccg tcgtaaaacg catgcccggg accgtaggac cggccgcctt
     1741 tcttctcgca gaccgacagg ctgaactcgc cgttatccgg ccgccacagc ttgacatagt
     1801 acgactgccc cggcaaagcc ggaacctcgc cgtgtcgcca ggtgaaggaa tccgcggcat
     1861 cgacggcctg gtcacgggcc ggcccgatcc gctcgtgcgt gattgcatca tggattgagc
     1921 agtacaaggc cgcgttgtcg aaggtccaga cggtgacctt caccacgtcg ccggtcgcca
     1981 caaacgtctg ccggatttcg cggcaggccg ggctcttctc cgtcgcccgc acacggacga
     2041 aatatgcggc atcaacctga aacgttcccg gcgtgacgat atcttcgcga accggaacaa
     2101 aactaaacag cttcggccgg aaatagtaca agtcgcccag gaccagcgag tactcgccgg
     2161 ccgccaggtc cttgatctcg taatagccgt tgttgctgat ccgcgcccag gccaggttgg
     2221 tgctctctcc cgagcggcac tgggtgattt ccatctcata caggctggcg tcgtcgaaat
     2281 catgggcgta tccggcgata ctgcctgccc aggcccacga gacgcctgaa acaaagaacg
     2341 tacaagacaa aaccacgcga cgaaagcagt aagctgccat gaccgttcct cacctctgca
     2401 taacggcgga cggcaaggtc gcaggccgat cttcagaccc aagcgggcgg ccattcaggt
     2461 cctgcgcgct tgagttccca gggacacgcc gcgcaccgac ttcaatgccc atgatccatc
     2521 caacggttga cgtcaagaat cccgctccgc gaccggcctg tcaacacaga atggcacaac
     2581 cacggaccaa tatcaactct ggccctgaca gcttgtcccc accgacgcct tggcgccctc
     2641 ttcaacgccg gagcatggct gcctgagagg tggtgacagg cccattctcc gccaccgccg
     2701 tcccctcaag gaacgcggga attcgtcccc atgagggcac aacgggcggg tccaggggca
     2761 cgatcgcaga gtctctcagg atggccctgg ctttctccga aagcacgaac cggacgaact
     2821 cctgggcaat cgttcgattc cggcagccct ccgtgatggt gatgccaaac gaaacgcagg
     2881 aacccgatat ggtttcaaca cctccgcggt aactcggcac ttcggtctgg gccttcgcat
     2941 attgactctc aagaccaggg tcaccgaggt ttatggccgg cggcagactt acgaaaggca
     3001 gatggtgatc aaccacgtga cagcggtaca gaaacgcata atcataggcc gcgctcgttt
     3061 ccagcaacga cagcaaggtc agggcgtcct ccttgacgtg cctcggatca caaccggcct
     3121 tcagttcctc gtaaagtcgg cgatgaccgg ttttcgttgc gtagtgcttc tccgcgagct
     3181 tccagcaaag catcgcgaag tacccgcaag ggtcctgagt ggggtcggcg tgcgagtagt
     3241 gaacgtcctt tcgcagcagg atctcgtacc agttgtccgg tgtgatctct gaagcatact
     3301 tgccggcgtc ggtgtgcccg atgaccatct cggtggtgcc gaacttgacg acccatgtgg
     3361 ccgtatgcgg attgagcatt ctttcgacca ggcgggcgtc ggccaccgcc gccacatcgc
     3421 acttgcgcag cggaaccagt cgcaggagca ccacactgcc ttgaaactca agctcaatgt
     3481 taacgtcggg gtgctccttc tcgaactcgc tcttgatgcg ggtgaagctg tgtgccaggg
     3541 catccgcggc gaacacccgc agcgtcttct tctgctcgcc cttcagtcga agaagaagta
     3601 ccatggcgat aatgacggcg atcagaccca gcagtttcag cgttcgacct cgagcctggc
     3661 ccactgacat ttcctgcaac ctcaagaaac ccgccttgtt cgacgaccgt acggacggcc
     3721 gaccgccgga ttggcggcgg cccacgaccg acttcgtgta tgctaccccg acgagccggc
     3781 ggcaaccggc gccgcatcct cgccggggaa tatcaatacg cgtcgggatg gaatccggat
     3841 tcgcacgcgt tgcccgggtt ccagcctcat cgttgcttct cgccggctcg acaactgccc
     3901 ttcgagccgc acttggtctg aaagcgatcg catgagcacc gtgcacgtac aggcatgccg
     3961 tcggaccgcc tcgatgaccg cggggcttcc ctcctctgtg cacgaaccgg ggccgtcggc
     4021 aacctcaggt gtgatttcga cgtcttgtgc ccggatcccc aaccagcagg ctcgatcggg
     4081 cgtgatcggc tcgccatggg cagccgcaat cataagatcc ccgactcgaa agagccgttc
     4141 accctccgcc ggccctgctc caacgcattg cccgcggaga atgttttcga cgagcagaaa
     4201 cctggccacg ggcaggtttg ccgggttctc gtagacaaac cggggttgtc caacctgctc
     4261 gatcccgccg tcaatcatga tgccgatccg gtctgccaag gcaaatgctt catcgaggtc
     4321 gtgcgtcacc agaacggtcg tcaatcgcag ccgacgctga acgtcgcgca tctccacatg
     4381 gagttgccgc cgcgtcgagg tgtccaatgc cccgaacggc tcgtccagga acagcacgcg
     4441 aggctccacg gcaagtgctc gcgccagcgc aaccctctgc ttttcgccgc cgctgaggct
     4501 ccggacatcc cgccggctca acagcgattc gatacggagc atgctcgcaa ccgagcgtat
     4561 ccggcggtcg atctccgtag agggcgtgtt cctgatgcgc aacccgaagc cgatgttctc
     4621 gggtacggac aggtggggaa acagcgcaag atcctgtgga acgtaggaga aacgacgctg
     4681 ttcgggaggc gcggtcgtga tatccgcgtc ttcgacaaga atgcggccgt gagcgggcgg
     4741 cttgacgccc atgatcgttt caagcaggag ggtcttgccc gcaccggatg gtccgatcag
     4801 cgcgaagcat tcgcccgcac tgatcaacag gctcaccttc ttcagcgaga agtacccgta
     4861 tcgggcaatg acgtcgcaga cctggatcat gagcttctcc gcataagcag gcccaccatg
     4921 atcaccacga ccaccgtgac gaacgtcagg accaggctga gcgcaatggc gcgatccagg
     4981 ctgacggacg ccagattcag gtagatgctg accggcatcg tttctgtctt gcccttcacc
     5041 gcgccggcaa tggttgagga tgccccgaag tcgcccatag cgcgaaccca gcccagcaca
     5101 aacgccgcaa tgatgccgtt tcgagccagc ggcagtgaga cgtgtcgaaa agcctgccag
     5161 ggcgtgcagc ccagaaagcg cgccacctgc tccagccggg cgtcgaccgc cgaaaacgtc
     5221 gagcggacca cgagaaccga gatggcgtac gccaggaaga actcggccag aatgatcccc
     5281 ggcacctcga acacgaaacg gatcaggtga tcttctatcc acgttccggc ctcggttcga
     5341 aagataagca gcagcgccac gccaagactg atcggcgaca tcacaatagg cacgatcagc
     5401 aggctctcca ccagccatcg tcccggaaac tggatgcgag ccagagccag gccggtagga
     5461 atggccagca agatcgcagc gactgatgcg aggcccgacg ttatgaagct gaggtaaatg
     5521 gcgtgctgga cgtcgtgctc ggtcaatgcc cgcagcggcg ccttggcgcc ggtgtaggcg
     5581 ccctgaacca gaatcagcat cagcatcaac gtggtgctca cacccacggc cgtcagcaag
     5641 atcgcatcaa atcggtttct gatcatgtct ctttgagcac ccagaagccg ccgggcggca
     5701 actaccattc ctcagggagt ctccattcgc cgccgacggg agtatcaggg agcgcgtacg
     5761 tgcgcgcctc ctccagcgtc gtgacgtagt gccatcgtcg aaacgactcc ctgccttggc
     5821 cgttcaccag gaactcgata agcttcaatg cttgttcttt ttctttgctg aagaccgaaa
     5881 cggcgatggg aaggtagccg atacggggga cttcgttcgg cttgagcaag atggtttcga
     5941 tcttgtccgg ttgccagtag tggaacacct cccaaccgag aatcgcgtcc actgaaccga
     6001 tcgccacgag ttgagcagtc ttctcgcacg actcaacatg ggtgacgatg ttgggcttga
     6061 cccgagcagc gtaaccgttc cgctccaaca cctctgccgc atagagccct acgcaaaccg
     6121 agtcggggcg ggcaataccc actctcacgc ccggctttgc cagatcctcc agccccttga
     6181 tgcccttggg attcccggcc ggaacgttga tcgccggtat cagataggcc acacgacgtt
     6241 ccgtctccgg caggaccagc cgttcccgct ttgccatctc catgaaatcc gatgaccccg
     6301 gaaagtacag gtcgcccctg cgggccagtt tcatctccgc cagcatcttg ccggacccgc
     6361 cgaaatgcag cagcatcctg gttccagtcg cgttctcgta gacttgtgcc gcctcttcga
     6421 caggcggctt ggttgccgaa ccgacgaaaa gctccagtgt cacgccggac gcctgtccgc
     6481 tgctatcagg ccgagacgaa gtccccggcg aatgcttgcc gtcagacccc ggcctctcgt
     6541 tgcatcccac gaccaagaag aacatcaccg ccggccacga cgccaaacgc cttaacagaa
     6601 tgcatctccg ggacatggga gtgttcctcc gcgccctttc aatttcgcac gacctgcggg
     6661 atgacggaca tgatcttacc ttcgacttgc cctgggtcca accgccccgg aaaaacgtca
     6721 aagccgtttc agacaccaga tcgggacttc cggtttcatt cgtcccccac gcctgcctcg
     6781 cgctggggga tggatcttcg actcgccgcg ccaggtcttc gatttcgttc ctccggtttc
     6841 actcactatg acgtcagcag acctcgagag ccctagtcga tcgccacctt tggggaggtc
     6901 atctctcggg ggagagccaa ccgcgtgaat cgctcaacgt tgccttggct gcccacccat
     6961 gcgctggctc gctcaagcga gagggccaac cccgcggtgt ccagcttgcc gggagcaagc
     7021 tcctgcacca ccttctgatc gctcagccgc agcactcgga aactcccgtc ggacccaagg
     7081 gccgccaggt aggccgagtc gttggtgaac tccgccgcgg tcagcttgac caacccgcgg
     7141 ccgcctggtc cggattcgcc cggccgtatc gaccacacgc tcttgtgccc ggcgtaaagc
     7201 gtcaaccggc acgagtcgtc ctgcacgatg aactgctccc cgtcgggcag ataccgaacc
     7261 agcgtcgcga tcggaccgac gcgctccttc tgccgccaca tgatcctgca tcgcaaatag
     7321 tcaatctcca tcacgccgct atccgtgccg acaagcaagt ggcgtccgcc cgggcgggcg
     7381 tcgagactga gaatccgcga ttccttcaga gtcttgccca gggtctggaa atcgctgcct
     7441 tcgtacagca tgtcatgttg acggtccggg atgcggcgca cgagggtgcc gtcacgaagg
     7501 gaaacaatgt ccacgacgcc cgtgcctccg caggcgatgt gagcaccgtc gtcgagaaag
     7561 gcggctgctc cgcgatcgtc cccaccgccg ggaacggaga ggattaacgg tgcggcggcg
     7621 gacgaggcgg tgttccacac ccggcagttc ccatccgtgc caaccgtggc gatatagtca
     7681 ccctcggggc tcactcgcag ctcgcgcacc gacccgcgat gcgaaccaag cacgcggatg
     7741 acgtcctgcg tccgctccac gtgatacacg cgccacacgt cggtc
//
LOCUS       DAOTPW010000266         7764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8325_length_7764_cov_6.065119, whole genome shotgun sequence.
ACCESSION   DAOTPW010000266 DAOTPW010000000
VERSION     DAOTPW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8325_length_7764_cov_6.065119"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1897
                     /locus_tag="P5316_18570"
     CDS             242..1897
                     /locus_tag="P5316_18570"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRR87019.1"
                     /translation="MQYAGGQASRSAAAFVAVVIIHFCPPCLSAAEQMPPGSQPARRP
                     NVVIFLADDLGFSDVGCYGGEIATPNLDRLAADGLRLTQMYNTARCWPTRGALLSGYY
                     AQQINRDPARQRPAWAALLPELLKPAGYHSYHCGKWHVDGKVLAGGFERSYHFEDWDR
                     YFTPKAHFLDDKQLPAVKPSDGYYATRAFAQYAIDFLAEHKEKHGSEPFFLYLAFIAP
                     HFPLHALPEDIARYRDRYLEGWDSIREARWRRLRALGIVDCGLSPLDPRFTPRYLKPD
                     LIDMIGPGEVEHALAWSELTPQQQRLQATKMAIHAAMVDRMDQEIGRVLDQLRKMGAW
                     DNTLVVFLSDNGADATLMVRGDGHDRAADPGSAASFLCLGPGWASVSNAPFRRHKIWV
                     HEGGISTPCIMHWPAAIRSKGALRHTPAHVIDLVPTVLELADVAAPETWKGLGRPPMP
                     GRSLVPLLDSDVEIQRDYLYWHHQGNRALRVGDWKLVSEAENNSMWELYNLKQDRIES
                     RDLAGQYPDRVSQMADMWKQLDQEFRRQSGAVPSTGPEPARQR"
     gene            2131..2589
                     /locus_tag="P5316_18575"
     CDS             2131..2589
                     /locus_tag="P5316_18575"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRR87020.1"
                     /translation="MSRHYGSLIGSVAFSLMVLLLLSGQGCPQGAGLGVPGVEEVRYT
                     NVTPAEAMALIQEHQDDPSFVILDVRSGEEFAAGHIEGAVSLCLLCSDPAFRDALADI
                     DRSLTFLVYCRTGRRSANASGIMAAEGFTNVYNMTGGIVEWQSLGLPVVK"
     gene            complement(2615..3397)
                     /locus_tag="P5316_18580"
     CDS             complement(2615..3397)
                     /locus_tag="P5316_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87021.1"
                     /translation="MTAINRILYPGAILATVLLLVLLKLPHLLLAVSLASGQLNGKPI
                     PVVNMPASRLPARTHVLLCGDLRLEVPAETSVQPQPPDDSCGFLLRLNGMVCRVMPPR
                     HQSAGQDVDEMEWAEELNRHGIDWQAAVCAKSARDFSFWMSSSQVESLRKQLEARMFL
                     SLFAERVEVVHNENLCGLLLMWDLDERPRMRLDYFTPDHRVSGVLLVALESATPEAVD
                     TTRAIVSSLRIEPPADNNVQVHESVAAATGLVPGRLPPASHR"
     gene            complement(3693..4592)
                     /locus_tag="P5316_18585"
     CDS             complement(3693..4592)
                     /locus_tag="P5316_18585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRR87022.1"
                     /translation="MKNIQKLGVYLNDEPGDEEALAFAGLLVKLGKPKFIDCIHVRGI
                     EDPVETPPPEPETLRQHVLECFSADSAKGVEVHVSEATGLQEMLRTARDHDLDMIVVG
                     RRLPHDQLALGSTFYRLARKTPCDVLVVPAGARPHLSRLLVLVDGSEHSRMALETALH
                     IARLAGKESQVLVQSVYGVPYGYRYAGMSIEQAQRHRETLQREKIGKFLADIDTSGVT
                     FDVVYTCSLNTAAAAHDMASARNMDAIVIGSRGATLPAVALLGATTERVLLEAPIPVL
                     VVKRKGETFRFLDAVLETFQAPG"
     gene            complement(4683..5222)
                     /locus_tag="P5316_18590"
     CDS             complement(4683..5222)
                     /locus_tag="P5316_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HRR87023.1"
                     /translation="MENQAQSALDQAPPGQQGCFITGGHTAHHDKFVERLICIIRWTV
                     RLLAVLMVFVIIMGVIDVVWVLYQRLVAPPFMLLEISDILGTFGAFMAVLIAIEIFVN
                     ITVYLREDVIHIRIVMATALMAIARKVIILDMKEVSMQEMLGLAAVTLAMSIGYWLVV
                     GNPFASRSSADNHSTSQRR"
     gene            5527..>7764
                     /locus_tag="P5316_18595"
     CDS             5527..>7764
                     /locus_tag="P5316_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRR87024.1"
                     /translation="MESLIGKHWHHLPAHEVVDLLETDLNKGLDLFAVKRRQEHFGPN
                     AVTARKGAGPLRRFLLQFHQPLVHILLVAALITAVLKEVVDAGVIAGVVLINAIVGFI
                     QESKAAKAIEALAKAMVTEATIIRGGRRLRVSARELVPGDIVLLQSGDKVPADIRLLE
                     SRDLQIDESILTGESVPVHKTAEILEHDTPLADRTNMAYSSCLVTYGQGTGVVVAIGD
                     ATEVGRISELIRAAPSLATPLTQKIARFSHVLMYSILVLALLTFGVGILRGESALLMF
                     KAAVALAVGAIPEGLPAVVTITLAIGVSRMARRRAVIRKLPAVETLGSTTVICSDKTG
                     TLTENQMTVRQIASGGIIAEVTGGGYAPEGQILAEGNAVDISSHVALRECLTAGLLCN
                     DAILVNKEGRWSVQGDPTEAALLVAAGKGGLSRESLSQQLPRIDVVPFESEHQYMATL
                     HDGGADSCIVYLKGAPEVVLQRCREALDSTGNRVPLDRDQVTAAAAELAGQGLRVLAF
                     ARSEQPPGTRHIAHRDLRDEMTFLGLQGMIDPPRAEAIEAVAKCRAAGIRVKMITGDH
                     ALTAAAIAKQIGIGVDSRPGDAFPQALTGPELAACSDEKLIDVADRTSVFARVSPEQK
                     LRLVEALQARGQIVAMTGDGVNDAPALKQANIGVAMGISGTDVAKEAADMVLTDDNFA
                     SIEAAVEEGRGVFDNLTKFIAWTLPTNIGEGLIILAAVFVGTTLPILPVQILWINMTT
                     AVLLGL"
BASE COUNT         1484 a         2278 c         2475 g         1527 t
ORIGIN      
        1 aaggccccag aacggattct ggggccagcg acaatatggg taccggggcc aacgggatct
       61 caagtggaac ggggccgacg agggcgtcgg ccctacttgc tcccagcagg tgccgcggtc
      121 tgccggggtg gagccgactc tggctgagat ctcgttaacg ttgcggtata cgaggctgag
      181 ttgtaccatc gtgggccgca tacggcgctg cggctttccg gcgtggaggt agaaccgaat
      241 catgcaatat gcaggtggtc aggcgagtcg atcggcggcg gcgtttgttg cggtcgtgat
      301 cattcatttc tgtccgccgt gcttgtcggc ggcagagcag atgccgccgg ggtcacaacc
      361 agcacggcga cctaacgtgg tcatctttct cgccgacgat ctcggttttt ccgacgtcgg
      421 ctgttacggc ggcgagattg ccacgccgaa cctcgaccga ctcgcggccg acggtctgcg
      481 gctgacccag atgtacaaca cggcccgatg ctggccgacg cgaggggccc ttttatccgg
      541 ctattacgcc cagcagatca accgggaccc cgcccgacag cgacctgcct gggccgcgtt
      601 gttgcccgaa ttgctcaagc cggcggggta tcacagctat cattgcggga aatggcacgt
      661 ggacggcaag gtgctggcgg gcggatttga acgttcttac cactttgagg actgggatcg
      721 gtatttcacc ccgaaggccc actttctgga tgacaaacag ttgcctgcgg tcaaaccgag
      781 cgacggctac tatgcgacga gggcgttcgc ccagtacgcg atcgactttc tcgccgagca
      841 caaggagaaa catggcagcg agccattttt cctttacctg gcgtttattg ctccccattt
      901 cccgctgcac gcactccccg aggacattgc ccgatatcga gatcggtacc tggagggctg
      961 ggactcgatc cgggaggcgc ggtggcggcg gttgcgggca ttgggcatcg tcgactgcgg
     1021 tctctcgccc ctcgatccga gatttacccc gcggtatctc aagcctgatc tgatcgacat
     1081 gatcggtccc ggtgaagtgg agcatgccct tgcatggagc gaattgacgc cgcaacagca
     1141 gcgtttgcag gcaaccaaga tggctattca cgcggcaatg gtagaccgga tggaccagga
     1201 gatcggccgc gttctggacc aactgcgcaa gatgggagcg tgggataaca ccttagtcgt
     1261 tttcctttcc gataatgggg cggacgccac gttgatggtc cgtggggacg gccacgaccg
     1321 agcggcagat cccggctcgg ctgcgtcttt cttgtgtctg gggccagggt gggccagcgt
     1381 ctcaaacgct cctttccgcc ggcacaagat atgggttcac gagggcggca tctcgacgcc
     1441 gtgcattatg cattggccgg cggccatcag atccaaggga gcacttcgtc acacgcccgc
     1501 ccacgtgatc gacctggttc ccaccgtatt ggaattggcg gacgtggcgg ccccggagac
     1561 gtggaagggc ctgggtcgcc cgccgatgcc gggcaggagc ctggttccgc tcctggattc
     1621 cgacgtcgaa atccaacggg actaccttta ctggcaccac cagggcaacc gcgccctacg
     1681 ggtgggggat tggaagctgg tatcggaggc ggagaacaat tccatgtggg aactgtacaa
     1741 tctgaaacaa gaccgcattg aatccaggga cctcgcgggg caatatcctg acagagtgag
     1801 tcagatggcg gacatgtgga aacaactcga ccaggagttt cgccgtcagt cgggagcggt
     1861 cccttcaacc ggaccggaac ccgcaagaca gcgctgacgc ggagcggtct gcattctcag
     1921 gtcaggtttt cgaagccgcg ggcaagtgtt cagtcgcggg acatgcgcgt cggatggaga
     1981 catccccagc cgaccaggtc catgattctg aatctgaagt cgcggaaacg gtgcctttat
     2041 ccagattggc attgatactt gccgatgaaa ttgaacgcct tgagtattgt tggcgtggac
     2101 agtccgcatc cataaaggag caggttgaat atgagtcggc attacggatc gctgatcggt
     2161 tcggttgcgt tttcgctgat ggtgctgctg ttgctgtcgg ggcagggatg cccgcaaggc
     2221 gcggggctcg gggtacccgg agtggaagag gtgcggtaca ccaatgttac cccggccgaa
     2281 gcgatggcat tgattcaaga gcatcaggac gacccgagct ttgtcattct cgatgtccgc
     2341 agcggggaag agttcgcggc cggtcatatc gagggcgcgg taagtctttg tcttctgtgc
     2401 agcgatccgg cttttcgcga cgcgcttgcc gatatcgaca gatccctcac cttcctggta
     2461 tactgccgaa ccggtcgccg aagcgcgaac gccagcggta tcatggccgc ggaagggttc
     2521 accaacgtct acaacatgac gggcggaatc gtagagtggc agagtctggg tctgccggtc
     2581 gtcaagtagc cgccggcctt gagcggatcc gacatcaccg atgcgaagcc ggcggcaacc
     2641 ggcctgggac aagccccgtc gccgcggcga cggactcatg gacttggacg ttgttgtcgg
     2701 cggggggctc aatgcgcaac gagcttacga ttgcccgggt cgtgtcgacg gcttcaggcg
     2761 tcgccgattc gagagcgacc aacagtactc cgctgacccg gtgatcgggc gtgaagtagt
     2821 cgagtcgcat gcgcggccgt tcgtcaagat cccacatcaa cagcaagccg cagagattct
     2881 cgttgtgaac cacttcgacc cgctcggcaa acaaggaaag aaacatcctg gcctccagtt
     2941 gtttgcgaag cgattcgacc tgcgatgagc tcatccagaa agagaaatcc cttgcgctct
     3001 tggcgcagac ggcggcttgc cagtcgatcc cgtgtcggtt caactcctcg gcccattcca
     3061 tttcgtcaac atcctggccg gcggattgat gccgcggggg catgactcga cacaccatgc
     3121 cgttgagcct caggagaaat ccgcacgagt cgtccggcgg ctgcggttgg acgctggtct
     3181 cagccgggac ctccaggcgc aggtcgccgc acaggagcac gtgggtccgt gcaggcaacc
     3241 tggatgccgg catgttgacg accggtatcg gtttcccgtt gagttggccg gacgcaagac
     3301 tgacggcgag aagcagatgc ggcagcttca acagaacgag cagcagaacg gttgccagaa
     3361 tggctcctgg atacaggatt ctgttgatgg ccgtcattgc ggttctccct tgtcggacgg
     3421 cgtggtgggc ttatgcgcgc ggcacgaccg gtagcggccg ttgggctgag gattcctgca
     3481 ggcctttggg ggcaccccgt gtgcaggcgg acccgcctgt ggcggttcga tggccgcctg
     3541 gcaagatccg gttccacaac tcaccccatg attagatgca caggggggtg atgttgttac
     3601 gtggtttttg ggaccacgtg ggattttttt tggatggtca gcgggcgcag acccgcctgc
     3661 ataccggttt gcgggcccgg acgccggctg ccttacccgg gagcctggaa agtctccaag
     3721 acggcgtcaa gaaatcggaa ggtttcgccc ttgcgcttga ccaccagcac ggggatggga
     3781 gcctccaaca aaacccgttc cgttgtggcc ccaagcaggg ccactgccgg cagcgttgct
     3841 ccacggcttc cgatcacgat tgcgtccatg tttcttgccg aagccatatc gtgggcggca
     3901 gcggccgtgt tcagggaaca cgtgtaaacg acatcgaagg tcacgccgct ggtgtcgatg
     3961 tccgcaagga actttccgat tttttcccgc tgcagtgttt cgcgatggcg ttgcgcctgc
     4021 tcgatgctca tcccggcata gcggtagccg taggggacgc cgtacaccga ctggacgagt
     4081 acctgcgact cctttccggc gagccgggcg atatgcagtg cggtctccag tgccatcctg
     4141 gagtgctccg agccgtcgac cagaaccagc aaacgactca gatgagggcg cgcgcctgcg
     4201 ggaaccacca gcacgtcaca aggggtcttg cgggccaggc ggtagaacgt cgagcccaat
     4261 gccaactggt cgtgcggcag tcgacgtccg accacgatca tgtccagatc gtggtccctg
     4321 gcggtacgaa gcatctcctg gagaccggtc gcctcggaca catgcacctc gacgcccttt
     4381 gccgagtcgg cagaaaaaca ctccagaacg tgttggcgga gggtttcggg ttcaggaggc
     4441 ggcgtctcca ccgggtcctc gatgccgcga acgtgtatgc aatcgatgaa cttgggcttc
     4501 cccagcttca ccaacaggcc ggcaaaggcc aatgcctcct cgtcgcccgg ttcgtcattc
     4561 aggtacacac ccagtttctg aatattcttc atgggaacac ctcactgtct ggagcaccgt
     4621 caggcatctt tttcgccact tgcgagcgat gacgactcgc tttcgtttcg gcccctggtc
     4681 tgttaccgac gctgcgaagt cgagtgattg tctgccgagg acctggacgc aaacgggttt
     4741 cccacgacaa gccagtagcc gatgctcatc gccagggtaa ccgcggccaa gcccagcatt
     4801 tcctgcatgc tcacctcttt catgtccagg atgatcactt tccgggcgat cgccattaag
     4861 gccgttgcca tgacgatccg aatatgaatc acgtcctcgc gcaggtagac ggtaatattg
     4921 acgaagatct cgatggcgat caacacggcc atgaaggcgc cgaaggttcc caggatatcg
     4981 ctgatctcca ggagcatgaa gggtggagcg acaaggcgct ggtaaaggac ccagacgacg
     5041 tcgatcacgc ccataataat gacaaagacc atcagcacgg ccagcaaccg gaccgtccag
     5101 cgaatgatgc aaatcagacg ttccacgaac ttgtcgtggt gagccgtgtg ccccccggtt
     5161 atgaagcagc cttgttgccc gggcggggct tggtcaagcg cagattgtgc ctgattctcc
     5221 atgacggtat tccggaacgc tttcgcgcgc gggtcggtat cctctcgtca cacggcccca
     5281 ctgccgatcg catgtggctt atcatgatac gtccagcgat caggtttagc cagagaggga
     5341 tctgaggacc ggcagcttgc ttctggtcac ccttggacag cggttgcaag ggacagagac
     5401 ggcgtcggct ggacgtgcag tcaggcttcg acttgggccg gcggctcgcg ccggtgctct
     5461 acaaccccat ttgaagactg gcagcgcgcc acccccgggc atagaatacg ccttggagga
     5521 gccctcatgg aatcactcat cggcaagcac tggcaccacc tgcccgctca tgaggttgtt
     5581 gatctcctgg agaccgacct gaacaagggg cttgatctgt tcgcggttaa acgccgccag
     5641 gagcacttcg gtccgaatgc cgtgactgcc cgcaagggtg ccggccccct gcgtcgcttc
     5701 ctgctgcagt ttcaccagcc tctcgttcat attcttctgg tggccgcgct tattaccgcc
     5761 gttttgaagg aggtcgtgga cgcgggtgtc atcgcggggg tcgtactgat caacgcgatt
     5821 gtgggcttca tccaggaatc gaaggcggcc aaggccatcg aagcccttgc aaaggccatg
     5881 gtcaccgagg ccacaatcat tcggggcggc cggcggctgc gggtttcggc gagggagctt
     5941 gtgcccggtg acattgtgtt gctccagtcc ggggacaagg ttccggcgga tattcgcctg
     6001 cttgaatccc gtgacctgca gatcgatgaa tcgatattga cgggcgaatc cgtgcccgtg
     6061 cataagaccg ccgagatcct cgagcatgac acccctcttg ccgatcgcac caacatggcc
     6121 tactcatcct gcctggtgac ctatgggcaa ggcacggggg tcgtcgtggc gatcggcgat
     6181 gcgacggagg tgggtcgtat ctccgagctg attcgcgctg ccccgagcct ggcaacgccg
     6241 ctcacgcaaa agatcgcccg tttcagccac gtgttgatgt acagcatact ggtgctggcc
     6301 ctgctgacgt tcggtgtcgg cattctccgg ggcgagtcag ccctgctgat gttcaaggcg
     6361 gccgtcgcat tggcagtcgg ggcgatcccc gagggactgc ctgcggtggt gacgatcacg
     6421 ctggcgatag gcgtgtcgcg catggcaagg cgacgcgcgg tgattcgcaa gcttccggcc
     6481 gttgaaaccc tcggcagcac gacggtcatc tgctcggaca agaccggaac gcttaccgag
     6541 aaccagatga ccgtgcggca aatcgcctcc ggcggcatta tcgctgaggt gacgggtggt
     6601 ggttacgctc cggagggcca gatcctggcg gaaggcaacg cggtagacat ctcgtcacac
     6661 gtggcactcc gtgaatgcct gacggcaggg ctgctatgca atgatgcgat actcgtgaac
     6721 aaagagggac gatggagcgt tcaaggcgat ccgaccgagg cggcactgct tgtggcggcg
     6781 ggaaagggtg gtctgtctcg cgagtcgctc agccaacaac ttcctcgcat tgacgttgtg
     6841 ccgtttgagt ccgagcatca gtacatggcc acgcttcatg acggcggtgc cgattcatgt
     6901 attgtctacc tcaagggggc tccggaggtg gtgcttcagc ggtgccgcga agccctggac
     6961 tcgaccggca accgcgttcc acttgatcgc gatcaggtaa cggcggctgc cgcggaactg
     7021 gcggggcaag gtcttcgggt cttggcgttt gcgcggtccg agcaaccgcc ggggacgaga
     7081 cacatcgctc accgtgacct gcgtgacgag atgacgttcc tgggccttca ggggatgatc
     7141 gacccgccac gagccgaagc gattgaggcc gttgccaagt gccgggcggc aggtattcgg
     7201 gtcaagatga tcaccggcga ccatgccttg acggccgcgg cgatcgcgaa gcagatcggg
     7261 attggtgtcg attcgagacc cggcgatgcc tttccgcagg ccttgaccgg accggaattg
     7321 gcggcctgct ccgacgagaa gctgatcgac gtagcggacc gaacaagcgt gtttgcccgc
     7381 gtcagccccg agcagaagct gcggctggtc gaggcgttac aggctcgcgg tcagattgtc
     7441 gcgatgacgg gcgacggggt gaacgatgca ccagccctca aacaggccaa cattggggtc
     7501 gccatgggca tcagcggaac cgacgtagcc aaggaagcgg ccgacatggt gctcaccgac
     7561 gacaacttcg cctccatcga agcggccgtc gaggaaggtc gcggcgtgtt cgacaacctg
     7621 acaaagttca tcgcatggac cttgcccacg aacatcggcg agggcctgat tattcttgcg
     7681 gccgtgttcg tgggaacgac tctgccgatc ctgccggtgc agatcctctg gatcaacatg
     7741 accacggcgg tactcctggg cctg
//
LOCUS       DAOTPW010000267         7733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8361_length_7733_cov_4.364678, whole genome shotgun sequence.
ACCESSION   DAOTPW010000267 DAOTPW010000000
VERSION     DAOTPW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8361_length_7733_cov_4.364678"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..703
                     /locus_tag="P5316_18600"
     CDS             113..703
                     /locus_tag="P5316_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87025.1"
                     /translation="MCERQEVAVGILLLGAGLVFVILGVKLYKALVPISFAAVGLVIG
                     IHVPVSGEIAQWACGIALAAVLGIGSSYVDKLAVALLAGGWTGVFAASLAVTFGIQQS
                     LSLAIGAVFLFAVASLALPLYRQVIAYVTSLQGAMLTIGGLIAVLHAFPKFWAGMRGI
                     VAESSYVAPFFVVSLTIMGYYLQSAETRQIESGMSG"
     gene            767..1825
                     /locus_tag="P5316_18605"
     CDS             767..1825
                     /locus_tag="P5316_18605"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87026.1"
                     /translation="MTRKSSPLPGKRIRNRIKRSPRRLRLALVGCGGMGQHHARSLLG
                     ISEVQVVALCDIIKSRCLSFCKQFFGPKGFKPSVYTDFYKMIARESLDAVLLVTPHAL
                     HYPHAKAALQKGIHVLSEKPMVTDAAQARELVALAESRQRLLAIAFQAPVSAEFAYIA
                     ELIRSGTLGRIELVDAHVAQRWKTFTKGTWRQDPKLSGGGQLYDSGAHMFNAMMWLVN
                     SPVKRVFAMADRCGTKVDINGTVSLVFENGCLGSVACSGNATVPGDTGVTLFATEGTI
                     KTGIWGEKLEHFDKAGNKILYPYVPYKTVPPERNFVDAILGRDTLRCPGRYGILLAEL
                     MDAIYESIKTGHPVDVRH"
     gene            1992..2780
                     /locus_tag="P5316_18610"
     CDS             1992..2780
                     /locus_tag="P5316_18610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR87027.1"
                     /translation="MRGPEVGVVLDSLGQPVKESLRLASRLGLRRIEIPAVTGEVSPE
                     SLSQTGRRHLQRYVAGLGLQLSALGGDLGGSRFGDSSAIEERLDRTRRIIEMAADLRV
                     PVLTTHLGRFDSNALRRGDLTGVVRELADMADRTGTFVAFETGGTDPSEMASLLKQID
                     CEWLGVCYDPASLLIDGYDPITSVEPTAGKILIARARDAVSGNERRPGHETPLGHGEV
                     DWARYLAFLEQSGYNNAAFLRRTESDRPLQDIADAKARLEAMLR"
     gene            complement(2839..5841)
                     /locus_tag="P5316_18615"
     CDS             complement(2839..5841)
                     /locus_tag="P5316_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87028.1"
                     /translation="MRRYFKTQIAGLGLLAACLQPAAAPGQHPLLSSSAHWLDVPAGE
                     YNDIAISPDGARLYLLDVAHDRVIVYDTTTDSVAGTIVFDRWLEGLAVTPNGARLVVA
                     ASEQYVYMVNTSTLTIIGSDDAWEFRGGPVAITPNSAVAYVAGSWDIYVVQLGAGGPQ
                     IVDTTPGDTTTDGLVGANYDDWTEFDNAAGVLVSADGNTLFVPIWHNPYHVIRGIANG
                     TNHTGLTLATQSLVHEYDPPLRVTLSADGSRLYDSYGVVRRASDLALLGRVSLYTDPE
                     AVTFDASMSPADVAVDPEGRYIYYAGFAWSYGQPDMATYGGEQLVVASHDDYEMIDLD
                     QDESNGLTCIRLPASPGGSSGTYSAHVMKISPDGDRLYMAAGSSGVLVVEFTHGGSGV
                     WSVRPSRGGDDDVTITVTGGGFAAGTTMSLERTGFPTIRASRTFVVGATTLKAVFDLT
                     GRAHGAYDVVLTTPDDSQTRLAGAFVIEAARESASLEIIGEATLFSSGASSVHVVCHN
                     TGNIDLLNPVISVQLTAGTQYRVELPATVPGGLTQLDDEFSEAVASHPEYVWVSRIGA
                     QDSYSFWLHVAGPENVAPLHWDVNLEATIGYLQSATDSSSAKLATRFSPIGDACTIFD
                     AAATNIHREFAERGYEVEFEEVFDIIEDAFVQSAADYGVSKLEEVVARGLGQMVVLAV
                     IPEAAPIYLIMFDGINDLRSCYLMYRTYLGFNALFAWDPNDKVSHTGVNGCIYGNETL
                     RYIIHFENLPEATAAATDVVITDTLDENLDYSTFTLVGSSHSSVLTTNLSTETGVVTF
                     TFRNINLPPNDTPPEGEGWVEYTIRPVADLASGTQIRNQASIVFDRNDPIVTPEVVHT
                     IDRTAPTSSVAALPARTSGDTIALQWSGSDAGAGVMDYTVYVSVDGGAYSAWLSETTS
                     TTATYEGAIGHRYGFYTIARDGLGNTESAPGQPDATTTLVARGRGGCGAMGPGGLVIL
                     AAGVLGLGFIRARVLCRRRWRRRSRSGP"
     gene            6220..7659
                     /locus_tag="P5316_18620"
     CDS             6220..7659
                     /locus_tag="P5316_18620"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR87029.1"
                     /translation="MNRLLRSLCVVASLANAAQAGNDVRFVDGELRFWNRQSEIWGAQ
                     VPDWQRLVSDPQAIRDTLQILADRGVNTIGICLQNPEGKGTLFAPDGRPADVATAGRF
                     TQLTALIRDHAMACVISLFSSDRSCWLKSPEAYRQAAQEAVRLLAVRHTTVFVVGDLF
                     GDSRWSSACPCRLDDPAQVVELYRQMRKTNDETLVGIPADCIHGTTDRQEAPFLYVAD
                     SPDSLAAFVAASPPGNNLPKGIARVQTYQFHRFKGDPDPKALDRFLADVEKRRLAVKA
                     PLPPTPADAPAAPLTPEEKAEGFVPLFNGHNLDGWTTLRPDWSTWSVEHGAIKCSGAD
                     GQWLRSRRRFASFILRFDFKIVENGNSGVFVWSPLEARASRFGMEMQIMGVQTDTPNP
                     NTSGAIYGVLPPSKLPDVRINDWNTAEIACRGSRVRVTLNDRTIQDFDADEVPKLKGR
                     LRRGFIGLQDHASPVWFRNIRIKELNDAR"
BASE COUNT         1441 a         2488 c         2324 g         1480 t
ORIGIN      
        1 cccgtgaccg tcacgcgtca ggaccggtca ggtcgccctg aggcccggat agattgtgaa
       61 gggtgatgac atgttgactt catttccgtc aaccgtgagg gaaacagtgc ttatgtgcga
      121 gcgccaggag gtggccgttg ggattctgtt gctcggcgcc ggattggttt tcgtaatcct
      181 gggtgtgaaa ctgtataagg cactggtgcc gatcagcttt gcggcggtag gccttgtgat
      241 cggcattcac gtgccggtgt cgggagaaat cgcccaatgg gcctgcggaa tcgcgctggc
      301 ggccgtcctg ggcatcggca gctcctacgt cgacaagctg gccgtcgcgt tgctggcggg
      361 cggatggacg ggcgtctttg ctgcctccct ggcggtcacg ttcggtatac aacagtcgct
      421 ctccctcgcc atcggcgcgg tgtttctgtt tgccgtcgcc tctcttgcgc tgccgcttta
      481 tcgacaggtg atcgcctacg tgaccagcct gcagggcgcg atgctgacca tcggcggtct
      541 gatcgccgtc ttgcacgcct ttccgaaatt ctgggcaggg atgcgcggca tcgtggctga
      601 aagcagctac gtcgccccgt ttttcgttgt ctcgcttacc ataatgggct actatctcca
      661 gtcggccgag acccggcaga tagaaagcgg aatgtcgggt tgatcgctcg atcccccggc
      721 gggcgaaagg ccaacgttct gatttccgtt cgagaggaga gcagtagtga ctcgcaagag
      781 tagcccttta cccggcaaac gaatccgcaa ccgaatcaaa cgttcgcctc ggcggctccg
      841 gctcgccctt gtcggttgcg gcggaatggg acaacatcac gcccgaagtc tcctcggcat
      901 atccgaggtc caagtcgtcg ctttgtgtga catcatcaag agcagatgcc tctccttctg
      961 caagcagttc ttcggcccca agggcttcaa gccctcggta tacaccgatt tctacaagat
     1021 gattgcccgt gaatccctgg atgcggtcct cctggttacg ccccatgcct tgcattaccc
     1081 gcacgccaag gcggccttgc agaagggtat ccacgttctc agcgagaaac ccatggtaac
     1141 cgacgccgcc caggcccgcg aactggtcgc cctcgccgaa tccaggcaac gcttgctggc
     1201 gatcgccttt caggccccag tcagcgcaga gttcgcctac atcgctgagt tgatccgcag
     1261 cggcaccctc gggcgaatcg aactggtcga cgcgcatgtc gcccagcggt ggaagacgtt
     1321 caccaaaggg acctggcggc aggaccccaa actctccggc ggtggacaac tctatgacag
     1381 cggcgcacac atgttcaacg cgatgatgtg gctggtcaac tcgccggtca agcgcgtctt
     1441 cgccatggcc gaccgctgcg gcaccaaggt cgacatcaat ggtacagtct ccctggtctt
     1501 cgaaaacggc tgccttggca gcgtcgcctg ttccggcaac gccactgtcc cgggcgacac
     1561 cggagtgacg ctgttcgcca ccgaaggcac cattaagacc ggcatctggg gcgaaaaact
     1621 cgagcatttc gacaaggctg gcaacaagat cctctatccc tatgtcccat acaagacagt
     1681 gccgccggag cggaacttcg tggatgcgat cctgggtcgt gacaccttgc gttgcccggg
     1741 tcgttatggc atcctgctgg cggaattgat ggatgcgatc tacgaaagca tcaagaccgg
     1801 ccacccggtc gacgtgcggc attaaggagg catctaggct tttagacttc tagactctta
     1861 ggtgcccggc gatcttgctg tcagggcggg caggtgggtt tgttccgcca gtctcttcct
     1921 gcgaaaacct aaacgtctaa tagtctagag gtctagagga ccaacagcct tctcgatcgg
     1981 aggtcttttc cgtgcgaggt cctgaagttg gggttgtgct ggacagtctc ggccaaccgg
     2041 tcaaggagtc gctccggttg gcgtcgcgac tcggcctgcg tcgcatcgaa atccccgccg
     2101 ttaccggcga agtcagtccc gaatcgctct cgcagaccgg ccgtcgccac ctgcaacgat
     2161 acgtggccgg gctggggctg caattgtccg ccctgggcgg cgatctgggc ggttccaggt
     2221 tcggcgatag cagcgcgatt gaggagcgtc tggaccgcac ccgacgcatc atcgagatgg
     2281 ctgccgatct tcgcgttccg gtattgacca cccacctggg ccgcttcgac agcaacgcgc
     2341 ttcgccgggg tgatctgacc ggcgtcgtgc gcgaactggc tgacatggcc gaccgtaccg
     2401 gtacgttcgt ggccttcgag accggcggca ccgacccctc tgaaatggcc tccctgctca
     2461 aacaaatcga ctgcgaatgg ctcggcgtgt gttacgatcc ggcctcgctg ctgatcgacg
     2521 gctacgatcc catcacaagc gtcgaaccga cggccggaaa gatccttatc gcccgcgccc
     2581 gcgatgccgt cagtggcaac gagcgacgcc ccggccacga aaccccgctc ggccacggcg
     2641 aagtcgactg ggctcgctac ctggcctttc tcgaacagag cggctacaac aacgccgcct
     2701 tcctgcgccg caccgagagt gaccgccccc tccaggacat cgccgacgcc aaggcccggc
     2761 tggaagccat gttgcggtga cgaacacggc cccggcgttt cgatcagcgc aacaccacga
     2821 ctggcagcca cgcgctgatc acgggccgct tctcgaccgc cgcctccagc gtcgccggca
     2881 aagaacacgt gctcgaatga acccaagacc gagcacgcct gcagccagaa tcaccagtcc
     2941 gccaggcccc atggctccgc agccgcccct gccacgggcg accagcgtgg tggtggcatc
     3001 gggttgaccg ggggccgatt cggtgttgcc cagcccatca cgggcaatgg tgtagaagcc
     3061 gtagcggtgc ccgattgcgc cttcatacgt ggcggtcgtg gatgtcgtct cgctcaacca
     3121 ggcactgtaa gcgccgccgt ccaccgagac gtagaccgtg tagtccatga cccccgcccc
     3181 cgcgtccgat cccgaccact gcagtgcaat agtgtctccg ctggtccgcg cgggcagagc
     3241 ggcaacgctg ctggtcggcg cggtccggtc gatcgtatgt acgacctccg gtgtgacgat
     3301 cgggtcgttg cggtcgaaca cgatcgatgc ctggttgcgt atctgcgtgc cggacgcaag
     3361 atcagcaacc gggcggatgg tgtattcgac ccagccctca ccctcgggcg gagtatcgtt
     3421 gggcggcaga ttgatgttgc ggaaggtgaa ggtcacaaca cccgtctccg tgctcaggtt
     3481 ggtcgtcaaa actgaggaat gactgctgcc gacgagcgtg aacgttgagt aatccagatt
     3541 ctcatccaga gtatccgtga tcaccacgtc tgtggcggcc gccgttgcct caggcaggtt
     3601 ctcaaagtga atgatgtatc ggagcgtctc gttgccgtag atacagccgt tgacaccagt
     3661 gtgcgacacc ttgtcgttgg ggtcccatgc gaacagggca ttgaacccca ggtacgtgcg
     3721 gtacatcagg taacaggacc tcaggtcgtt gatgccgtcg aacatgatca ggtagatcgg
     3781 ggccgcctca ggaatcaccg cgagcaccac catctggccc aggccccgag cgacgacttc
     3841 ttcgagcttc gacaccccat agtcggccgc cgactggacg aaggcatcct cgatgatgtc
     3901 aaagacctcc tcgaactcca cctcgtaccc ccgttcggcg aattcgcgat gaatattggt
     3961 ggctgccgca tcgaagatcg tgcaagcatc gccaatcggg ctgaaccttg tggcgagctt
     4021 ggccgagctg ctgtcagtgg ccgattggag atatccgatc gtcgcttcca ggttcacgtc
     4081 ccaatgcagc ggggcgacgt tttcaggccc ggcgacgtga agccagaagc tgtatgaatc
     4141 ctgtgcgccg atgcggctga cccacacgta ttctggatgg ctggcgacgg cttcactgaa
     4201 ctcgtcgtcc agttgcgtca gtcctcccgg gacggtggcc ggcagttcga cccggtactg
     4261 cgttcctgcg gtgagctgca ccgaaatgac cggattgagc aggtcgatgt tgccggtatt
     4321 gtggcagacg acgtggaccg aactggcccc gctggagaaa agagtggctt cgccgatgat
     4381 ttccagggac gctgattcgc gggccgcctc gatcacaaat gctccggcca gtcgcgtctg
     4441 ggaatcgtcg ggagtagtca gaacaacgtc gtatgccccg tgtgccctgc ccgtcagatc
     4501 gaaaactgcc ttgagcgtcg tggctcccac gacgaacgtt cgagaggccc tgatggtcgg
     4561 aaagcccgtt cgctcaagcg acatggtcgt gccggccgca aaaccgccgc cagtgacggt
     4621 gatcgttacg tcgtcatctc cgcctcgcga aggccggacc gaccagacgc ccgatccgcc
     4681 gtgcgtgaac tcgacaacca gcactcccga actgcccgcg gccatgtaca gccggtcgcc
     4741 gtccggcgaa atcttcatga cgtgcgcact gtaggtcccg ctcgatccgc ccggagaggc
     4801 cggcagtcga atgcaggtca agccgttcga ctcatcctgg tccaggtcaa tcatctcgta
     4861 atcgtcgtgc gaggccacga caagttgctc gccgccatag gtggccatgt cgggctgccc
     4921 gtaggaccag gcaaaaccgg cgtagtagat ataccggccc tcggggtcca cggccacgtc
     4981 ggcagggctc atgctcgcgt cgaatgtgac cgcctccggg tctgtataaa ggctcactcg
     5041 tcccagcaac gccagatcgc tcgcccggcg cactacgccg taggagtcat acagcctgct
     5101 gccgtcggcc gagagcgtca cgcgcaaggg cggatcgtac tcatgcacca ggctctgggt
     5161 ggccaacgtc agccccgtgt gattcgtacc gttggcaatg cccctgatca catggtaggg
     5221 gttgtgccag atcggaacga aaagcgtgtt gccgtccgcc gagaccaata cgccggcggc
     5281 gttgtcgaac tccgtccaat catcgtagtt tgcgccgaca agaccgtccg tcgtggtgtc
     5341 gccgggcgtc gtatcgacaa tctgcggacc gccggcgccg agttgaacga cgtaaatatc
     5401 ccaggaaccg gcgacgtacg ctacggcgct gttcggggtg atggcaaccg gtccgccccg
     5461 aaactcccac gcatcatcgc ttccgatgat ggtcaacgtg cttgtgttga ccatgtaaac
     5521 gtattgctcg ctcgcggcga cgaccagacg cgccccgttg ggagtgacgg caagaccctc
     5581 cagccagcgg tcaaagacaa tcgttcccgc cacgctgtcg gtcgtcgtgt cgtaaacgat
     5641 gacacggtcg tgggccacgt ccagcaggta caggcgtgcc ccgtccggac tgatcgcaat
     5701 gtcattgtac tcccccgcgg gtacgtcgag ccaatgagca ctgctgctca gcaaaggatg
     5761 ttgccccggc gctgcggcag gctggaggca cgccgccaga aggccgagcc cggcaatctg
     5821 tgtcttgaaa tatcgtcgca tggacccgct tctcctttcg atctgatccc atccttgacg
     5881 gcgggacgcg aggcgggcat ggcaggacga tgcgcccatc agccgcccgt gcagacgccg
     5941 ccggagcagg aggaggcgat gataccgccg aacgcagtga tcagtgatct ttgatcgtcg
     6001 aaaacgctcc cggcgatgtc gcgatcctac atccgcggca tgcgggccgc aatgacgatc
     6061 cgtgcatccg acctgtcacc cgctcctcga tcgggatgag cggtcggcct cggaatcagc
     6121 taatccgcag ttgaaccttg ccggtggcgg cggtacaagt gttgtcgcgc gaacggctcg
     6181 gacgaccggt gcgtgtcgtc gcggaaaggc aagatccata tgaatcgact gctgcgttcc
     6241 ctctgtgtgg tggcctctct ggcaaatgcg gctcaagccg gtaacgacgt ccgtttcgtg
     6301 gacggcgagc ttcgcttctg gaatcgtcag tccgagatct ggggcgctca ggtacccgat
     6361 tggcaacgac tggtcagcga tcctcaggcc atccgcgaca cccttcagat cctcgccgat
     6421 cgcggagtca atacgattgg gatctgcctc caaaaccccg aaggcaaggg tactctcttc
     6481 gccccggatg gccggcccgc cgatgtcgca acggccggac ggttcaccca gctcacggct
     6541 ctgatccgcg atcacgccat ggcctgtgtg atcagccttt tctcctccga ccgatcctgt
     6601 tggctcaaat cgccggaagc ctaccgtcag gcggcgcagg aggccgttcg cctgctggcg
     6661 gtcagacata ccacggtgtt cgttgttggc gacctgttcg gcgacagccg ctggtcctcc
     6721 gcctgtccct gtcggttgga tgatccggca caagtggtcg aactctaccg gcagatgaga
     6781 aagacgaacg acgagaccct cgtcggcatc cccgctgatt gcatccacgg cactaccgac
     6841 cgacaggaag cgccgttcct ctatgtggct gactcgcccg atagcctcgc ggcattcgtc
     6901 gccgcttccc cccccgggaa caacctgcct aagggcatcg cgcgcgttca gacgtatcag
     6961 tttcaccgct ttaagggcga ccccgatccc aaggcgctcg atcgctttct cgccgacgtc
     7021 gaaaagagac gcttggccgt gaaggccccg ctccccccga caccggctga tgcccccgcc
     7081 gcgccgctga cgccggaaga gaaagccgag ggcttcgtgc ccctcttcaa cggccacaac
     7141 cttgacgggt ggaccacgct ccgacccgac tggtcaacct ggtcagtcga gcacggagcc
     7201 atcaagtgtt ccggtgccga cggccagtgg cttcgctccc gcagacgctt cgcctctttc
     7261 atccttcgct tcgacttcaa gatcgtcgag aacggcaaca gcggcgtgtt tgtctggtcg
     7321 ccgctcgagg cccgtgcctc gcggttcgga atggaaatgc agatcatggg tgtgcaaacc
     7381 gacaccccca accccaacac cagcggggca atctacggcg tgctcccgcc ctccaagctg
     7441 cccgatgtca gaatcaacga ctggaacacc gccgaaatcg cctgccgcgg ctccagggtg
     7501 cgggtcacgc tcaatgaccg gaccattcaa gattttgacg ccgatgaggt gcccaagctt
     7561 aagggccgtc ttcgccgcgg ctttatcggc ctccaggatc acgccagccc cgtctggttt
     7621 cgaaacatcc ggattaagga actcaacgac gcaaggtagc gccgggcacc atgcccacgg
     7681 ctctgcgtgg gcatgcgcct ccaaatgaat cctgaagcag gtagggcagg tca
//
LOCUS       DAOTPW010000268         7669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8437_length_7669_cov_5.586420, whole genome shotgun sequence.
ACCESSION   DAOTPW010000268 DAOTPW010000000
VERSION     DAOTPW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8437_length_7669_cov_5.586420"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..2225
                     /locus_tag="P5316_18625"
     CDS             102..2225
                     /locus_tag="P5316_18625"
                     /inference="COORDINATES: protein motif:HMM:NF041647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HRR87030.1"
                     /translation="MNRYVRIGAIYRKELLNILRDRRAMLAMIIIPVVMYPALMLGFV
                     RAVESDEAKLRSQTFVVETPTMEEADNLLARVVLAQDADAKDQPTFDIRPGNTPLTLL
                     GDEVQLHVELQVSPRPSPFPERLTVHIAFNEVNARSRTAMEQLTAALNRYRERMTRDS
                     LERLLESRPLMMPSTEPVVDLVLNPVSIETFSTATGQQRGGWALGLIVPVILVLMTIT
                     GAIYPAIDLTAGERERGTLEALLATPVPVLHLVTAKFLVVATIGLLAAFVNVLSVGAT
                     MHFGGLTKAFANAEAPVQFPASTLPIIVFSMIPFALLSSAILVAVCSFARSYKEAQTY
                     VTPVIILALVPAIAATLPSIQLKGIFLVMPVGNMVLLARELFQQTCTWPQVVIVLLST
                     TLYAAAAVGVAARLFGQEAVLFADAGSYRTLLLRRLMQPSPAPTVAQAVVLIALLFPA
                     VFFINSLFSNLLLERLISGLIWLAVVQFGGLFLLLPLAVAAFLKIDLVNTFRLRLPPA
                     RAWLAALLIGVSSWAVATECFALQTRVIPPSEALAQLDRVLSEGAENASLRTIVLLLA
                     LIPAVTEEFLFRGFLLSGLGRASGKWTAVIGAGLIFGAFHFVVDKIPVTALMGMLLGY
                     LCWQSRSLWPGILAHAMHNSALMVLPNMPRAAAWLGLAGIDREPERLLPIHVVIPAAV
                     LLLAGLAILATLRQRRSSTANGFSG"
     gene            complement(2327..5596)
                     /locus_tag="P5316_18630"
     CDS             complement(2327..5596)
                     /locus_tag="P5316_18630"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HRR87031.1"
                     /translation="MRFEFFRAASVCLVLAAVHVSPLPAGEASRERQRVEIGWHDDFR
                     TDSGWLRENSDAPDHGDHAAIAFGSDGAVVSVASSNRATAWTRTTNPIWVNVFPFLEV
                     RYSIRESGSGAQVWLLLSDDSTGPVTPGALNPENPLAGDHEALVGPLSRDQDRAVFDL
                     RALFPSDRVARISLRITTEAAPTTVELRRLAFWASDPRLPSPQATRPTCTAILPPEDD
                     FSTSRPSQRGVWQPVQLPQRDVISAAWLTSALETPVDWPAGKDIERGGILFRLGDADR
                     AAMCSGVMERESLDVSGSSQGCELALLLATRMFGSGVAWYGQASVKPRTTITSPHQLA
                     VRLEYEDGSTSIHLPWSVRRKTWAVDRSPDVYIVPMDADKSLVRFSVVDGMSYGQVFL
                     LAASLNSTSSPSFPQLRPKAEPRRTRQAPVPVTSPTQWHRAGNRFTVNNAWLRLAAEV
                     ETGLRLEQLGLVPFDREILNAKSPAPFVEVLGEDGRVLSMRFEAGNAGDTAGGARLDF
                     RWSLAPSEHKLSLSVSVEDAGRIRMVPTLHNQTAQTWKTALRIAQLRECRISPIPGDS
                     GYLVGTRNALQSRVPTSIRKDYSGVWPLPLVDLFAEQAGGGLGLFVIDSNLLPKAIEF
                     KQTDSGADIAIEIRELTVPPDGHLVLPPVTLMAHTGDWHDLFNAYRAEAVAETPPDDK
                     RLEESFYCRRDYPLGGTGHLFTPLSLRYTPERLIAESNEAFGGIHMIDISGWAYSRAT
                     GRVGDYLSNDLGGLSELRRMVERSHAEGIKVGLYFEGYLIDRRSDLARKALPDWQSVD
                     KTGKPRWWSGDMEFFACPGVEAWRRELAGRIAKVAHETGADAVYVDQFGLLGPGKACW
                     APNHGHPIPSNPILEERAMLRTIREALDKQAPGTAIYIEYTPVDGLMDLVDAAFDYGL
                     WDVDAGWHPASLPLYRYVFPKLASFEMVGHGIRPIPVEVDDLHRCIFHGLGIWLKGRA
                     DSWYTPQFRELARQAYPIFREHADIFRSRQCEPLVPTLQEDLYANRFAGRDRTIITLY
                     NAGCSSVAGELMQVPLPPEWSVVSLLPRGEADAQRDKEQVTIRGTVAPRSVAVFLLSA
                     P"
     gene            complement(5664..7292)
                     /locus_tag="P5316_18635"
     CDS             complement(5664..7292)
                     /locus_tag="P5316_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRR87032.1"
                     /translation="MSKSTLPLIMASASIAAGQAGLAAQGSALPVIPQPARVERADGV
                     FVIDENTAIVVDAASMAEAYKLQDYLAPATGLRLKLVENAGSQGAITLRIADSFAALG
                     EEGYALIVAPDGILIGARARAGLFYGIQTLRQLLSPAIFRLARVEGVRWEVPCVRIED
                     QPRFRWRGLLVDTARHFVPKSALLKFIDTMALHKFNSLQLHLTDDQGWRIEIKKYPKL
                     TEIGAWRDETLVGHAHAKPWQFDGRKHGGFYTQDDIREIVRYAADRHINVVPEIEMPG
                     HARAAISAYPHLGCNPDTPLKPWTVWGVCPDIFCPTDQTVAFLQDVLAEVMELFPSPY
                     IHIGGDEAVKDQWKASPVVQARIKELGLKDEAEMQSWFVKQMDAFLTRKGRRLVGWDE
                     ILEGGLAPGAVVMSWRGEKGGITAAKSGHDVVMAPTSHTYLDYYQGPKDSEPLAIGGD
                     LPIEKVYAYEPIPADLSEEEARRVLGTQGQLWSEYIPNPRHLEYMAYPRAAALAEVAW
                     SAKGQRNFDSFLERLRQHVQRLQVMDVNFRPLDR"
BASE COUNT         1388 a         2495 c         2334 g         1452 t
ORIGIN      
        1 atggccggct gccggcggct gacggacgtt ttcctccggc tcgtcggcca ggcaatggcg
       61 ccgacgctgc cgttcaagcc gccgggcgaa gaggtggtcg catgaaccga tatgtgcgaa
      121 tcggggccat ctaccgcaag gaattgctca acattctccg cgatcggcgg gcgatgctcg
      181 caatgatcat cattccggtg gtgatgtacc cggcgctcat gctcggcttc gtgcgagcgg
      241 tcgaaagcga tgaggccaag ctgcgatccc agaccttcgt cgtcgagacg cccacaatgg
      301 aagaagcgga caacctcctg gcgagagtgg tactcgcaca ggacgccgac gccaaggatc
      361 agccgacttt cgacattcgg cccggcaaca cgccgctcac gctgctcggc gacgaggtgc
      421 agcttcacgt ggagttgcag gtgtcgcctc gaccaagccc ctttcccgag cgactgacgg
      481 ttcatatcgc cttcaacgag gtcaatgccc gcagccggac ggccatggag caactcacgg
      541 cggcgctgaa ccgatatcgc gagcgaatga cgcgcgattc gcttgagagg cttctcgagt
      601 ctcgccccct catgatgccg agcaccgagc ccgtcgtgga cctggtgctc aacccggtga
      661 gtatcgagac cttttcgacg gccaccgggc aacagcgcgg cggctgggcc cttggcctga
      721 tcgtccccgt gatcctggta ttgatgacca tcaccggggc aatctatccg gcgatcgatt
      781 tgacggccgg cgagcgggaa cgcggaacgc tcgaagcttt gcttgcaaca cctgtgcccg
      841 tcctgcacct ggtaacggcc aagttcctgg tggtcgcaac catcggactg ctggccgctt
      901 tcgtcaacgt gctgagcgtc ggggcgacca tgcacttcgg cggcctgacc aaggcatttg
      961 ccaacgccga ggcgcccgtt cagttccccg cctcgacctt gccgatcatc gtgttctcga
     1021 tgattccgtt cgccttgctg tcctccgcta ttctcgtcgc ggtgtgcagc ttcgcccgct
     1081 cctataagga ggcccagacc tacgtcacgc cggtgatcat tctcgccctg gtgccggcga
     1141 ttgctgcgac gctgcccagc atccagctca agggcatctt ccttgtgatg ccggtgggca
     1201 acatggtcct tctcgcccgc gagctgttcc agcaaacctg cacctggccg caagtggtca
     1261 tcgtgttgct gtccacaaca ctgtacgcgg cagcagccgt cggcgtcgcg gcgcgacttt
     1321 tcggtcagga ggccgtgttg tttgccgatg ccggttccta tcggacgctc ctgctacgac
     1381 gactcatgca gccgtcgccg gccccaaccg tggcccaagc cgtcgtcctg atcgccttgc
     1441 tgtttccggc ggtcttcttc atcaattcct tgttctccaa cctcctgctg gagcgcctca
     1501 tcagcggatt gatctggctg gcggtcgtcc agttcggcgg attgttcctg ctgctgccgt
     1561 tggccgtggc ggccttcctc aaaatcgacc tggtcaacac gttccgcctg cggctgccgc
     1621 cggcacgggc ctggcttgcg gcgctgttga tcggagtctc gagctgggcc gtcgccaccg
     1681 agtgcttcgc cttgcagact cgcgtcatac cgccttcgga agctcttgcg caactggacc
     1741 gcgtgttgtc agaaggagcc gagaacgcat ccctcaggac gatcgtgctt cttctggcgt
     1801 tgatcccggc tgtcaccgag gaattcttgt ttcgcggctt tctcctcagc ggcctcgggc
     1861 gtgcatcagg caaatggacg gccgtgatcg gcgctggcct gatcttcggg gccttccatt
     1921 tcgtcgtgga caagataccg gtcaccgcac tgatgggcat gttgctcggg tacctgtgct
     1981 ggcagtcacg ttcgttgtgg ccgggaatcc tggctcacgc catgcacaac tccgctctca
     2041 tggttttgcc caacatgccc cgcgcggccg cctggctggg actggcgggc attgaccgcg
     2101 agccggaaag gctcttgccc atccacgtgg tgatccccgc ggcagtcctc ctgttggcag
     2161 gcctggccat cctggcaacg ctgaggcaac gccggtccag cactgccaac ggcttctcag
     2221 ggtagggcat ggtcgaggtc gctacagcga ccgagacccg ccgcagaacc tttcggccgg
     2281 tcgattggac cggcaagatg ccggtccagg ggcctgttac agtccgtcag ggtgcagaga
     2341 gcagaaacac ggccaccgac cgcggtgcga cggtgccacg aatcgtcact tgttccttgt
     2401 cgcgctgggc gtcggcctcg ccgcgcggga ggagactgac caccgaccat tccggcggaa
     2461 gcgggacctg catcagttcg ccggccactg acgagcagcc ggcgttgtac agggtgatga
     2521 tcgttcggtc acggccggcg aaacggttcg cgtagaggtc ctcttgcagc gtcggaacca
     2581 gcggctcgca ctgccgcgaa cggaagatgt ccgcatgctc acggaagatc gggtatgcct
     2641 gccgggcgag ttcgcggaac tgcggcgtgt accatgaatc cgccctgccc ttgagccaga
     2701 ttcccaatcc gtgaaagatg cagcggtgca ggtcgtcaac ctcgaccgga atgggccgga
     2761 tcccgtggcc gaccatctcg aatgatgcga gcttgggaaa cacataccgg tacaagggca
     2821 gcgaggccgg atgccagccg gcatcgacgt cccacagacc atagtcgaac gcggcgtcca
     2881 cgagatccat caggccgtcg accggagtgt actcgatgta tatcgcggtg cccggggctt
     2941 gcttatccaa tgcttcacga atggtccgca gcatcgcccg ttcttccaga atggggttgg
     3001 aggggattgg atgcccgtgg ttcggagccc agcaggcttt gcccggccca agcaggccaa
     3061 actgatcgac atagacggca tccgcgcccg tttcgtgcgc caccttggcg atcctgccgg
     3121 ccagttcacg ccgccacgcc tcgacgcccg gacatgcaaa gaactccatg tcccccgacc
     3181 accagcgcgg cttgcccgtc ttgtccaccg actgccagtc gggcaacgcc ttcctggcca
     3241 ggtccgaccg ccggtcaata aggtacccct caaagtacag gcccaccttg atcccctcag
     3301 cgtgtgagcg ctccaccatc cgacgaagct cgctcagccc gccaagatcg ttcgagagat
     3361 agtcgccgac gcgcccggtc gcgcggctgt aggcccatcc agagatgtcg atcatgtgga
     3421 ttccgccaaa ggcctcgttg gactcggcga tcaggcgctc gggggtgtac cgcaacgaca
     3481 agggggtgaa caggtgtcca gtccccccca aggggtagtc tcgccggcag tagaacgatt
     3541 cttcaagccg cttgtcatcc ggcggcgtct cggcaaccgc ctcggcgcgg tacgcattga
     3601 atagatcgtg ccagtctccg gtgtgtgcca tgagcgttac cggagggaga acaagatggc
     3661 catcgggcgg gacggtcagc tcacgaatct cgatggcaat gtcggccccg ctatcggtct
     3721 gcttgaactc gatcgccttg ggcaacaggt tcgagtcgat cacgaacaag cccaacccgc
     3781 caccggcctg ctcggcgaaa agatcgacaa ggggcaacgg ccatacgccg ctgtagtctt
     3841 ttcggatgga agtgggcaca cgactctgca gggcgtttcg tgtcccgacc agatagccgc
     3901 tatcgcctgg gatgggggaa atgcggcatt ctcgaagttg tgcgatacga agggccgtct
     3961 tccacgtctg ggcggtctgg ttgtgcagcg tcggaaccat gcggatgcgg ccggcatcct
     4021 caacggacac ggacaaggag agcttgtgtt ccgacggggc cagagaccaa cgaaagtcca
     4081 gacgagcccc accggccgtg tctccggcgt taccggcctc gaatcgcata gacaataccc
     4141 ggccatcctc gccgaggacc tcgacaaaag gtgcgggaga cttcgcgttg aggatttccc
     4201 gatcgaaagg cacgagccca agttgctcca gacgcagacc ggtctctact tcagccgcca
     4261 gacgcaacca tgcgttgttg acggtgaagc gattcccggc gcgatgccat tgtgtcggcg
     4321 aggtgacagg caccggagcc tggcgcgttc gtcgtggttc ggctttcggg cgcaactgtg
     4381 gaaatgacgg cgatgaggtg gagttcaagc tcgctgcaag cagaaacacc tggccgtagc
     4441 tcatcccgtc aacaacactg aaacgaacga ggctcttgtc ggcatccatc ggcacgatgt
     4501 agacgtccgg cgagcgatcg acggcccacg tcttgcggcg aaccgaccag ggaagatgaa
     4561 tgctggtgct tccgtcctca tactccagcc ggacggcgag ctgatggggc gaggtgatcg
     4621 tggtgcgagg cttgaccgac gcctgcccgt accacgcgac gccgctgccg aacatgcgcg
     4681 tcgccaacag cagggccaac tcgcagccct gtgagcttcc gctcacgtcc agactttccc
     4741 tctccatgac cccgctgcac atcgccgccc ggtccgcatc acccagccga aacaggatgc
     4801 cccctcgctc gatgtctttg ccggccggcc agtcgaccgg cgtctccaga gccgaggtca
     4861 gccatgctgc ggagatcaca tcgcgttgcg gcaattgaac gggctgccag acacctcgtt
     4921 gcgaagggcg gctcgttgaa aagtcatctt ctgggggcaa aatcgctgta caggtcggtc
     4981 gagtcgcttg cggcgatggc agacgcggat ccgatgccca gaatgcaagt cgccgcagtt
     5041 caacggtggt cggcgcggct tccgtggtga tccgcaggct gatccgggcc acccgatccg
     5101 atgggaatag ggcccgcagg tcgaatacgg cacggtcctg gtcacgcgat aacggcccca
     5161 caagcgcctc gtggtcaccg gcgagcgggt tctccggatt cagagcaccg ggcgtcaccg
     5221 ggccggtgga gtcgtcggac agtagcagcc agacctgtgc ccccgatccg ctttcacgga
     5281 tgctgtaccg gacctcaagg aacggaaaca cgttcaccca tatcgggttg gtggtccgtg
     5341 tccaagccgt cgctcgattc gaagaagcca cgctgacgac ggcgccatcc gaaccgaaag
     5401 cgattgcggc atgatccccg tgatccggcg catcgctatt ctcacgaagc cagccgctgt
     5461 cggttcggaa atcgtcgtgc cacccgattt ccacgcgctg gcgctcgcga gaagcctctc
     5521 cggccggcaa cggtgacacg tgaaccgccg caaggaccag acagaccgat gcagcccgaa
     5581 agaactcgaa tcgcatggca cactccgaga caaacccgcc cggtctggcg accggcccat
     5641 attcggtcac gcaatgccgg tgcttacctg tccagcggcc ggaagttgac atccatcacc
     5701 tgcaaccgct gcacgtgctg tcgcagccgt tccaggaatg aatcgaagtt ccgttgccct
     5761 ttggcggacc atgcgacctc ggccagtgcc gccgcgcgag gataggccat gtattcaagg
     5821 tgcctcgggt tgggaatgta ttccgaccag agctgccctt gcgttccgag cacgcgccgg
     5881 gcttcttctt cgctcagatc agccgggatg ggctcatacg cgtaaacctt ctcgatcggc
     5941 aggtcaccac cgatggcaag cggctcacta tccttcgggc cctggtagta atccaggtag
     6001 gtgtgtgacg tcggtgccat taccacgtca tgccccgact tggccgccgt gatcccgcct
     6061 ttctcgccgc gccacgacat gaccaccgct ccgggtgcca ggccgccttc cagaatctcg
     6121 tcccagccga ccaaccggcg cccctttctg gtcaggaagg cgtccatttg tttgacgaac
     6181 caactctgca tctcggcctc gtctttcaaa cccagttcct tgatgcgggc ctgcaccacg
     6241 ggactggcct tccactgatc cttgaccgct tcgtctccgc cgatgtgaat gtacgggctg
     6301 gggaaaagct ccatcacttc tgccagaacg tcctgcagga acgccacggt ctggtccgtc
     6361 gggcagaaaa tgtccgggca tacgccccac acggtccacg gtttgagcgg cgtgtcgggg
     6421 ttgcatccga gatgcgggta ggcggagatg gccgcccgcg cgtggccggg catctcgatc
     6481 tcgggaacga cgttgatatg ccgatcggcc gcataacgga cgatctcgcg gatgtcatcc
     6541 tgggtgtaga acccgccgtg tttgcggccg tcaaactgcc agggtttcgc gtgggcatga
     6601 ccgacaaggg tctcgtcccg ccatgccccg atctccgtga gcttgggata cttcttgatc
     6661 tcgattcgcc agccctggtc gtccgtcagg tgaagctgca gactgttgaa cttgtgcaga
     6721 gccatggtat cgatgaactt gagcagcgct gacttcggta cgaagtggcg agcggtgtcg
     6781 accagcaagc cgcgccacct gaagcgaggt tgatcctcga tccggacgca cgggacctcc
     6841 caacgcacgc cttctacccg cgctaagcgg aagattgcgg gcgacagcaa ctgccgaagc
     6901 gtctgaatgc cgtagaacag gccggcgcgg gctctggccc cgatcaggat gccgtcggga
     6961 gcgacgatga gcgcgtatcc ttcctcgcca agagcggcga acgagtccgc gattctcagt
     7021 gtgatggcac cttgcgatcc ggcgttctca acaagcttca ggcgcagacc cgtcgccggg
     7081 gcaaggtaat cctgcagctt gtacgcttcg gccatgctcg cggcgtcgac gacaatcgca
     7141 gtgttctcgt caatgacgaa cacgccgtcc gcgcgttcca ctctggcggg ttggggaata
     7201 accggcaagg cggagccttg agcggcgagc ccggcctgtc cggcggcgat gcttgcactc
     7261 gccatgatca gcggcagcgt tgacttggac atgtgaacct cctgtggtat tcaaaacgtc
     7321 agggcagatg cggccatgat aaccacccct gacaaagccg ggagcatgag agccctgcct
     7381 ggacccaact gaccggccgt tctgagggcc gtcttgatcc gctcgcaaat cgatgtcata
     7441 cggccctcgt tcgccggcac agaatccacc gactcctccg gcaaacggcc ggtgagcacg
     7501 tcccgccctg caaagtgttg cttcgttgct cgggatacgc cgactccact gttacgaccc
     7561 tgtcgctgcc gggtcaaccc cgccaccagg ccggatctca aatctcaaat cgccaatctg
     7621 aaatcagatc gttcggcagg tctccgccgc ctttggcggc ttcgaccat
//
LOCUS       DAOTPW010000269         7604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8516_length_7604_cov_4.415949, whole genome shotgun sequence.
ACCESSION   DAOTPW010000269 DAOTPW010000000
VERSION     DAOTPW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8516_length_7604_cov_4.415949"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..538)
                     /gene="cas2"
                     /locus_tag="P5316_18640"
     CDS             complement(209..538)
                     /gene="cas2"
                     /locus_tag="P5316_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRR87033.1"
                     /translation="MNLSAYRFMWLMVMFDLPVDTKAARREYAIFRKRLLKDGFTRIQ
                     YSVYVRHCASQENADVHVQRVEAAVPPDGEIRVLLITERQYERMRVFWGKRRKAPENP
                     PCQLELF"
     gene            complement(535..1461)
                     /gene="cas1"
                     /locus_tag="P5316_18645"
     CDS             complement(535..1461)
                     /gene="cas1"
                     /locus_tag="P5316_18645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HRR87034.1"
                     /translation="MIKRTIEISHEAAHLAVKLDQIQLLRHDASVKAPIASIPCEDIG
                     LVVVDHPGVTYSHAALARIIEFGGGVLICGRDHLPAGLLLPMISHTEGVWRIKDQLRA
                     SKPLHKRLWQQIVIAKIKAQARNLPSGEVSQGRLLALAGEVKSGDPSNVEAQAAKIYW
                     GAWLGDRPQAAWFRRRPEGKDPINGMLNYGYAAMRAAVARALVGGGLLPALGIHHKQR
                     GNNFCLADDLLEPLRPLVDACVRDLIDWNITEIDQRAKKHLLALLTRTVQVGDQMGPL
                     MVGLHRTVASLVACLRGESRRLDLPVGVRNDS"
     gene            complement(1508..5284)
                     /gene="cas9"
                     /locus_tag="P5316_18650"
     CDS             complement(1508..5284)
                     /gene="cas9"
                     /locus_tag="P5316_18650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HRR87035.1"
                     /translation="MICLGLDIGSNSVGSAWVDTEKREVHLAVSVFPAGVDEQEDKRG
                     APKNQARRQKRSQRRTLARRSARKRHLRSFLTQAGLLPTDPADLEMLFQANPWYLRRE
                     ALSRPLTPHEFGRIVVHLSQRRGAVGITTDPDDPNEGKVKDGMDRLDKLMRDRGPDTT
                     IGQLMADLIDERRQEHDGITWNEPIRNRQYRIPEDQQLFAGRELIRREFHRIVEIQRS
                     FKDSQLGPMLTDDVIRSLDDPTQTDTWRHQGLLFGQRRTYWDTGTLGRCVLEPTERCA
                     PIADRHASYFRVVETVNSILIERQGQPKRPLTPEERDKVVRLLRGPLFKKSRGRPEPK
                     STASVTDIKEALGIKPRDKTVKLNIEADEDREINTDWFHREIVHGAFTEAKWELLAED
                     QRESVNRAVLKFDPDQADDAARLRAGAERWWAMDAASADRLVEAWKRRPRLEKRLNLS
                     RRAILNLLPIMNRFDSVNNRWPTQQEARKLYARTLNDQHARRRYETGAPGLSAADRYY
                     MRLGKHQIVAGVPELPPAPTLSNPVVRKAIHEVRRHLVAYLRRFGRKPDRVVIEMARV
                     TKQSERQRNEALARNRHRDKIRKAIIKEILPAAFGEEEAARLSLSQQRAAVDRVVLAR
                     QQRNVCPYCGQGGLTDLLAARGDDLEIDHIVPYSRCGDNGLNNKVLVHRACNRGKTNQ
                     TPREWWGSAFDERIRFAEKVFKDIKPEKSDYFNKRDSDRKWENFTREVREGEEWKNSQ
                     LTDTAYAARQVAAYLADALYDGRGLPERGDGEGAQKVFFTMGRFTAMLRKDWQLFETL
                     KPPQRDSKHGELTAEEELRLAEKNRGDHRQHAIDAVTIALTDPTIKNTLASWAAQAAE
                     YYEQHRQWPRRKPTDPPWGDVPSFRKQILSRVYESFDSSELASGKRDERGAASVLIVS
                     HRPVKRRLVGALHEDTHYGPVVGPLPSHRTERIDTLFTNRISVDRLSPNHLRVPEGWD
                     ELSVRLDEAGVADARKRDLRKQLSAMKDPSPEKSGIVRDRALRDRIRKCLRANGLDPD
                     GFTANQIKQLVREGKLTMASGVPIKSVVLLRTNSDPVIIPRKQCDPASGKTVPHMDPD
                     NPGAPHPRTRRVYIGGNNHHVEIRERIRQRGGQAVAEWIGEVVTTMDAARRLHPPRNP
                     DGTRPEPRPAVDRSDNHDGVFVMSLAEGETIYARRKDRPEEPASYFVVCKLDKAGNSC
                     RIHFAPHWDARKASEQDRWAVTPGDLRLCGPEPDRPPYKVQVGPLGDVKRLEKD"
     gene            complement(5649..5915)
                     /locus_tag="P5316_18655"
     CDS             complement(5649..5915)
                     /locus_tag="P5316_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87036.1"
                     /translation="MEGTTSISLRSIILRELAANIIAHRGHTSTAPGTMMIHQARKER
                     GPAEGSEALRGGYQHPAFGIYHFLWLSPLLAAIMALLSIQEMFV"
     gene            complement(5956..7371)
                     /locus_tag="P5316_18660"
     CDS             complement(5956..7371)
                     /locus_tag="P5316_18660"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRR87037.1"
                     /translation="MSKRFVCTVVFLAASPGTSIALGQIRVWVADQGDNQGGNNRIME
                     IDPFNKKTPPDPDGTDCIILKTLPSPAGCFLDELDFDANYRMWAVVKDTCNQSIDGAR
                     RIDKDTGAVDIPPGLITPQFPGETAGGILEGLAWDGTGLWITAVRDGLTGNMLTRVSP
                     ADGSQIAPFTAPPLAGGKCNIPGTIAQGLLWEPGNNGYGWLWHSDVAARKLYKLDVSR
                     LFTNPTYNPADPNGLTVAEYSVPCEPKGLAWMDTKIWIAAPRGSNGGIFEFDPATGGT
                     VKLFSTPNWHLDGIAVYRPPGPEIKLSTEVITTSVWIAEGDPADSVFTVTNKGFGTLD
                     YTISDDVEWLTVTPDSGSSTGSPNSHTVSFVADGKLGGTYNATITVAGNAWNTDQHVA
                     VSMIIRTVGPDVDGDGDVDQRDFGLFQACLTGAGQTVQPPCGIADFNNDGLVNQGDMT
                     IFVDCLSGPDVLADKSCDPAA"
BASE COUNT         1331 a         2403 c         2282 g         1588 t
ORIGIN      
        1 tgctcgcagg tcggggtcga ttacacttcc ctgtctgagt ctggcgctga cgcttccgtt
       61 gcggcttgct cgcaggtcgg ggtcgattac actcgtggat gggctcgagc gcaaggtgtc
      121 tctgttgcgg cttgctcgca ggtcggggtc gattacacta gttcccgcgc aagcggtttg
      181 ccgcacagga cttaggcaac gaagggggtc agaaaagctc gagttggcac ggcggattct
      241 cgggggcctt gcgccttttt ccccagaaaa cccgcatccg ctcgtactgt ctctccgtga
      301 tgaggaggac gcgaatctcg ccatcgggcg ggaccgcggc ttccactcgc tggacgtgca
      361 catcggcatt ttcctggctt gcgcagtgcc gcacgtagac tgagtattgt attcgcgtaa
      421 agccatcctt gagcagcctc ttacggaaga tggcatactc gcgtcgagcg gccttcgtgt
      481 caaccggcaa atcgaacata accatgagcc acatgaatcg gtacgcgctg aggttcatga
      541 gtcattcctg actccaaccg gcaagtccag gcgtctgctt tccccgcgaa ggcaggccac
      601 caacgaagcc accgttcggt gcaggccgac catgagcggt cccatctgat cgccgacctg
      661 caccgtgcgg gtgagcagag cgagcaggtg tttcttggcc cgctggtcaa tctccgtgat
      721 attccagtcg atcaggtcac gcacgcaggc gtccaccagt ggccgcagcg gctcgagcag
      781 gtcgtccgcc aggcagaagt tgttccctct ctgcttgtgg tgaatgccaa gagctggtag
      841 caggccgccg cccacgagcg cgcgggccac ggccgcccgc atcgcggcat agccgtagtt
      901 cagcatccca ttgatcggat ctttgccctc cgggcgtcgc cggaaccagg ccgcttgtgg
      961 gcggtcgccc aaccacgccc cccaataaat cttcgccgcc tgagcctcga cgttcgatgg
     1021 atcgccggac ttgacctcgc ccgccagcgc gagcagtctg ccttgagaaa cctcgcctga
     1081 cgggagattg cgggcctgag ctttgatttt ggcgatgacg atttgctgcc agagccgctt
     1141 gtgcagcggc ttcgacgccc tgagctgatc cttgatccgc cagaccccct ccgtgtggct
     1201 gatcatgggc aataggaggc cggctggcag gtggtcccgg ccgcagatta gcaccccgcc
     1261 gccgaactcg atgatccgcg ccagggctgc gtgcgaatac gtgacgccgg gatggtccac
     1321 aaccacgagt ccgatatctt cgcaggggat cgacgcgatt ggggccttca ccgaagcatc
     1381 gtgacgtaac aactggatct gatccagctt caccgccaga tgcgcggcct cgtgcgagat
     1441 ttcgatggtg cgcttgatca cggcgcgggc gcctccgaat accatatcgg cgcgccggat
     1501 cgccatatca atctttctcg agccgcttca cgtcgccaag aggcccgacc tgaaccttgt
     1561 acggcggtcg gtcaggctcc ggaccgcaca gcctcagatc gcctggggtg acggcccacc
     1621 ggtcctgctc actcgcctta cgtgcgtccc agtgcggggc gaaatggatg cggcatgagt
     1681 tgccggcctt gtccagctta cagacgacaa agtacgacgc cggctcctcc ggtcgatcct
     1741 ttcgacgggc atagatcgtc tcgccctcgg cgagagacat gacgaaaacg ccgtcgtggt
     1801 tatccgaccg gtccaccgcc ggcctcggtt caggccgggt gccatccgga ttcctcggcg
     1861 ggtggaggcg cctggcggca tccatcgtcg tgacaacctc cccgatccat tcggccacag
     1921 cctgcccccc tcgctggcgg attcgctcgc ggatttcgac gtggtggttg ttccccccga
     1981 tgtagactcg cctggtccgg ggatgcggtg ctcccggatt gtccgggtcc atgtgcggaa
     2041 ccgttttgcc actggccggg tcgcactgct ttcggggaat aatgaccggg tcgctgttcg
     2101 tccgaagaag caccacgctc ttgatgggta ccccgctggc catcgtgagc ttgccctccc
     2161 tgaccagttg cttgatctga ttggctgtga agccgtcagg gtcgagccca ttggcgcgca
     2221 gacacttgcg gatgcggtcg cgcagggcac gatcccggac gatgccgctc ttttcgggtg
     2281 aggggtcttt catcgctgaa agctgcttgc ggagatcccg cttccgggcg tccgctacgc
     2341 ccgcctcatc cagcctgaca cttaactcgt cccacccctc gggtacgcgc agatgattgg
     2401 gactcaggcg atccacgctg atccgattcg taaacagcgt gtcaatgcgc tcggtgcgat
     2461 gcgacggtag cggcccgaca acgggaccgt aatgagtatc ttcatgcaac gctccgacca
     2521 gacgccgctt cacaggtcgg tgtgagacga tcagtacact tgcggcgcct cgttcatcac
     2581 gcttccccga cgccaactca ctcgaatcaa agctctcgta gacgcgcgag agaatctgct
     2641 tgcggaaaga gggcacgtcg ccccatggcg gatcagtcgg ctttcgacgc ggccattgcc
     2701 ggtgttgctc gtagtattcg gccgcttgcg cggcccacga tgccagcgta ttcttgatcg
     2761 tcgggtccgt cagcgcgatc gtgaccgcat caattgcatg ctgacggtgg tcgccacggt
     2821 ttttctcggc gagccgcagc tcttcctccg ccgttagttc gccgtgtttg ctgtcccttt
     2881 gtgggggctt gagcgtctcg aagagttgcc agtccttccg cagcatcgcg gtaaaacggc
     2941 ccatcgtgaa gaacaccttc tgagcgccct ccccatctcc ccgctctgga agtccccggc
     3001 cgtcatataa cgcgtcagcc aggtacgcgg caacctgccg agccgcatag gccgtatccg
     3061 taagctgcga attcttccac tcctcgcctt cccggacttc tcgcgtgaag ttctcccact
     3121 tgcgatcaga atcgcgcttg ttgaaatagt cgctcttctc aggcttgatg tccttgaaca
     3181 ccttctccgc gaagcggatc ctctcgtcga acgcgctgcc ccaccattca cgcggcgtct
     3241 ggttggtctt gccgcgattg cacgcccgat gcaccaggac tttgttgttc agcccgttgt
     3301 caccgcatcg gctgtagggg acgatatggt caatctccaa atcatcgccc ctcgcagcga
     3361 gaaggtcggt caacccgccc tggccgcaat aggggcacac attcctctgc tggcgcgcca
     3421 gaacgacgcg atccacggct gcacgctgct gactgagcga cagccttgcc gcttcttcct
     3481 ccccgaatgc ggccgggagg atttctttga tgatggcttt gcggatcttg tcccggtgtc
     3541 ggttccgggc gagagcttcg ttgcgctgac gctcggactg cttcgtcacc cgggccatct
     3601 caatgacaac tcggtccggt ttgcggccaa agcggcgcag gtacgccacc aagtggcggc
     3661 gaacctcgtg gatggctttc cggacgactg gatttgagag tgtgggcgcc ggcggcaatt
     3721 ccggcacgcc cgcgacgatc tggtgcttgc ccaggcgcat atagtaccga tccgcagcgc
     3781 tcaggcccgg cgcgccggtt tcatatcgcc gacgggcgtg ttgatcgttt agtgtcctgg
     3841 cataaagctt gcgcgcttcc tgttgcgttg gccaccgatt gttgactgag tcgaagcggt
     3901 tcatgatcgg caggagattc aggatagcgc ggcgggaaag gttcaaccgc ttctcaagcc
     3961 tggggcggcg cttccacgcc tcgacgagcc gatccgcaga ggccgcgtcc atggcccacc
     4021 atcgttcggc ccccgctcgg agcctcgccg cgtcgtccgc ctgatcgggg tcgaacttga
     4081 ggacggctcg attcactgac tcgcgttgat cctctgccag tagctcccat ttcgcctcag
     4141 tgaatgcgcc atgcacaatc tcacggtgga accagtcggt gttgatctcg cggtcttcgt
     4201 cggcctcgat gtttagcttg acggtcttat cgcgaggctt aatccccagg gcttccttga
     4261 tgtccgttac actggccgtg gatttgggct cgggtctccc ccgggacttc ttgaacagcg
     4321 gcccccggag tagccgtaca accttgtcgc gctcttcggg ggttagcggt cgcttcggct
     4381 ggccttgacg ttcgatgagg atactgttga ctgtctcgac gacgcggaag tagctggcgt
     4441 gccggtcggc gataggcgcg caccgctcag ttggctcgag gacgcaccgg ccaagggtac
     4501 cggtgtccca gtacgtccgg cgctgtccga acagcagacc ttgatgacgc caagtatccg
     4561 tctgggtggg atcgtccagc gaccggatca catcgtctgt caacatcggg ccaagttgcg
     4621 agtccttgaa ggatcgctgg atttcaacaa tacgatggaa ttccctccgg atgagctctc
     4681 gtccagcgaa tagctgctga tcttcgggaa tacggtattg ccgattgcgg atcggctcgt
     4741 tccatgtgat accgtcatgc tcttggcgac gttcatcgat caaatccgcc atcaattggc
     4801 cgatggtcgt gtccgggccc cggtctctca tgagcttgtc gaggcggtcc ataccatctt
     4861 tgaccttgcc ttcattcgga tcgtccgggt cggtggtgat accgacggcc ccgcggcgct
     4921 gcgagaggtg gaccacgatg cgtccgaact cgtgcggcgt cagtgggcgc gaaagggcct
     4981 cgcgtcgcag ataccacggg ttggcctgaa agagcatctc caggtcagcc ggatctgtcg
     5041 gaagaaggcc ggcttgagta aggaacgacc gcaggtgacg cttgcgtgcc gaccggcggg
     5101 ctagagttcg gcgttgggat cgcttttgcc ggcgggcttg gttcttgggg gccccgcgct
     5161 tgtcctcctg ctcatccacc ccggcaggga acacgctgac ggctaagtga acctcccgtt
     5221 tctcggtgtc tacccaggca cttcccacgg aattgctgcc gatatcgaga ccaagacaaa
     5281 tcatgttgcc atcctccgat tctggcgaaa acaccctctt gacaggacgg ggcgcgatcg
     5341 gctaatctga gagccagtgt aatcgacccc ggcctgcggt tttccgcaac aagagagaat
     5401 tcgcaggcac cgccttcggg cgaccgccgt agctcgtgta acccacgacc tgcggctttt
     5461 tgttatcgtc ggtgtggcca ctgggcccat acgatcctcg tcaaccccga actccgtcag
     5521 gttctgcatc gagaagcagt ctctcggggc gtacgccagt gattcgttga agacccaagg
     5581 gatggcccag atgtaatcat gcagtttgcg gggtgtattc cggaccggcc gtgccgtgaa
     5641 gcgcccgttc atacaaacat ctcctgaatg gacaacagag ccattatcgc cgcaagcaat
     5701 ggggaaagcc agaggaaatg ataaatgccg aaagctgggt gctgatagcc ccccctcagc
     5761 gcctcgctcc cttctgccgg tcccctctct tttcgtgctt ggtgaatcat catcgtaccc
     5821 ggcgcagtgc tggtgtgccc gcggtgagcg atgatgttgg ccgccagctc gcggagaatg
     5881 atcgagcgca ggctgatgct cgtagtcccc tccacatggt ccgccagggc aagccgggcg
     5941 gccgcggatg cccgctcagg ccgcgggatc gcagctcttg tcggccagga catccggacc
     6001 gctgaggcag tcgacaaaga tggtcatgtc gccttggttg accagcccgt cgttgttgaa
     6061 atcggcgatc ccgcaaggag gctgtaccgt ctgcccggcc ccggtaagac aagcttggaa
     6121 caaaccgaag tcccgctgat cgacgtcccc gtccccgtcg acgtccggac cgacggtgcg
     6181 tatgatcatg ctcacggcga cgtgctggtc ggtgttccag gcattgcccg caacggtgat
     6241 cgtggcattg taggtgccgc caagcttgcc gtcggccaca aaggacacgg tgtgcgaatt
     6301 gggcgacccg gtcgacgaac cggaatccgg tgttacggta agccactcaa cgtcgtcact
     6361 gatcgtgtag tccagcgtcc caaagccctt gttggtcacc gtgaagacac tgtcagctgg
     6421 atcgccctcg gcaatccata cggaggtcgt gatgacctcg gtgctcaact tgatctccgg
     6481 gcccggtggc cggtaaaccg caatcccgtc caggtgccag ttcggcgtgc tgaaaagctt
     6541 gacggtgccg cccgtggcgg ggtcaaactc gaagatgccc ccgttggaac ctctgggagc
     6601 ggcgatccag atcttggtgt ccatccatgc cagccccttg ggttcgcacg gtacgctgta
     6661 ttcggcgacc gtcagaccgt tgggatccgc cggattgtaa gtgggattgg taaacaaacg
     6721 ggacacatcc agcttgtaga gcttccttgc cgccacgtcc gagtgccata gccagccata
     6781 gccgttgttc cccggctccc agagcaaacc ttgagcaata gtccccggaa tattgcactt
     6841 gccacccgca agcggtgggg cggtaaaggg ggcaatctgc gatccatcag ccgggctgac
     6901 acgggtcaac atgttgcctg taagaccatc gcgaacggcc gtgatccaca accccgtgcc
     6961 gtcccacgcg aggccctcga gaatgccgcc cgccgtctcg ccaggaaact gaggcgtgat
     7021 cagtcccggc ggaatgtcga cggccccggt gtccttgtcg attcgcctgg ccccgtcgat
     7081 gctctggttg catgtatctt tgaccaccgc ccacatgcgg taattcgcgt caaagtcaag
     7141 ctcgtccaga aagcacccgg ccgggctggg caaggtcttg aggatgatgc agtccgttcc
     7201 atccgggtcc ggcggagtct tcttgttaaa cgggtcgatc tccatgatcc ggttgttgcc
     7261 gccctggtta tcgccctggt cagccaccca caccctgatc tggccgaggg cgatggacgt
     7321 gcccggagag gctgccagaa agacgactgt gcagacgaac cgcttggaca tgcgcgcatc
     7381 tcctcttgga agcaagcctc aaagtcgtca ttctcacact ggaccgccac gccgcgacta
     7441 cccctcctaa gctagcaatc ggcccgcaat gagtcaatca ttcattaaac gttggataag
     7501 gctgccttac gaaggcaggg catggtcgaa atcctcgcgg cggacgagac ctgccgacga
     7561 ttcctttttc agggatgcgc ccgccggcga tggtgctcgc cacg
//
LOCUS       DAOTPW010000270         7577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8542_length_7577_cov_6.495480, whole genome shotgun sequence.
ACCESSION   DAOTPW010000270 DAOTPW010000000
VERSION     DAOTPW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8542_length_7577_cov_6.495480"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="P5316_18665"
     CDS             complement(<1..841)
                     /locus_tag="P5316_18665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR87038.1"
                     /translation="MNRTNCAGRLIIIAAVGCLLGTSALKAQDTSQPFPIRADEARIA
                     EVLKDLDTKRAGMMNVPIEDARLLRFMALAANAKHVVEIGTSNGYSGLWICLALQSTG
                     GKLTTFEIDAGRAALARENFKRAGVESIVTLVEGDAHKEISRLKGPIDMVFIDAEKPG
                     YFDYLNKLLPLVRPNGLILAHNTTNLGSQMKEYIEAVTKNPDLETLFLKVSDQGMAVT
                     VKKPAPPKPAEKPQAAPDDKAEGKPVLGYINQIPPESDEQRALRHNKIAARRQGTPVM
                     VHRG"
     gene            1368..1556
                     /gene="rpsU"
                     /locus_tag="P5316_18670"
     CDS             1368..1556
                     /gene="rpsU"
                     /locus_tag="P5316_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRR87039.1"
                     /translation="MIKVKARGNESLEQMLRRFKKMCEKEGLTKEIKRTAYYEKPSER
                     RRRRIRKSVKRVERPMGR"
     gene            complement(1654..2919)
                     /locus_tag="P5316_18675"
     CDS             complement(1654..2919)
                     /locus_tag="P5316_18675"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR87040.1"
                     /translation="MSDSNLIETRNKESRRLWERSCQCIVGGGQAHKRPVDIMKLGGP
                     SFAARGKGARFWDVDGNEYLDYLLGYGPIVLGHCDPEVNQAVRRQMEEGTVFSIEHPL
                     EIELAEMLIQTIPCAEMVMFFVGGSAATMGAIRCARAHTGRERIIRCGYHGWLDWCVP
                     DNPGVPRFNRELSVAVPYNDLPALRNMLETAPGQVAAVLVESVQDNGPSPDYFPGVRQ
                     LCDEHGAVFILDEIKTGFRFGLEGAGPRFGAEPDLATFGKAMCNGYPAAVVVGKRSIL
                     EKRTDTHMAATFHGDLLSVVAALTTIRVLKERDGIDHFWRLGRRLMNGLNRLAREQEL
                     PIKFVGFAPMPVLKPADETDPTPCPKELRDEVLKQFCAAIQRRGVFATPHPWFLSLAH
                     TEEDIDKTIDVAAEAACELKHFLARGAAG"
     gene            3161..5047
                     /gene="nagB"
                     /locus_tag="P5316_18680"
     CDS             3161..5047
                     /gene="nagB"
                     /locus_tag="P5316_18680"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF002557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRR87041.1"
                     /translation="MLRKEPIPTAPEFTVLERIRVHICADDAEAAREVTRIIADLIRQ
                     NNANSRKTVLGLATGNTPINVYRELIRLHQQESLDFSRVVTFNLDEYYPMRPDSIQSY
                     RRWMQENLFDHINVKPENIHIPDGSIPESEVSAYCERYEEMIEQAGGIDIQILGIGRT
                     GHIGFNEPGSLIDSRTRLIDLDAVTRRDAAADFFGEANVPHRAITMGVGTILKARRIL
                     MLAFGEQKAPIIRRTVEGPVTHAVPATFLQNHPNAEIFIDRGAAGDLTRVESPWLLGE
                     IEWTPENQLRAVIWLSLKTGKSILKLDPEDYSGNHLSSLVRAVGSADRLNHRTHRRLS
                     ATIVTKNLMATKRRMLCFSPHPDDDVISMGGTLTRLVQRENEVHVAYMTNGSIAVADD
                     HALQLLDFWSDLNHTFKLDTESTAMFYDKVHRFLADKEPGEVDSFEVQEIKRLIRESE
                     ALAACRSMGIPTQNAHFLDLPFYQTGEVRKRPISEQDIEIVAGLMRAIRPDWVFVAGE
                     LSDPHGTHRLCAEAIFAALRRLSPEEKPVQVWLYRGAWQEWEPDVIDVVIPFSRTELM
                     HKIFAIFKHQSQKDKALFPGPYDDREFWQRAEARNTETAAIFNKLGLPEYYAMEAFVR
                     YDEF"
     gene            complement(5114..6679)
                     /locus_tag="P5316_18685"
     CDS             complement(5114..6679)
                     /locus_tag="P5316_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR87042.1"
                     /translation="MANQGHRQTRWTGLTVALAVAALAVVVSPTFGQTDARRVRQPNI
                     LVIMSDEHNASVMGCAGNRIAHTPNLDRLAERGVLFDAAYTTSPLCAPARLSFTLGKY
                     ISHIDVWSNSARLPPDDTPSLPHILNAAGYESFLCGKMHYDSEHRYGFAELGKADTNR
                     GTKSGALGRRRPDDLAPSGTISGRFDQFRPGDSSGPLNHDREVTAGVTRFLAGRKKTD
                     KPFFLLVGYLAPHFPLIVPARYWQPYRDKVPMPEIPPGYLDTLPRNYRHLRVGFELIG
                     LPEDTVRRGRELYYGLTQWLDGEIGKVLTALEASGLAEDTVIIYTTDHGENLGEHGLW
                     WKNCMFDSAARVPLIISWPKRWPGGQKRDKACSLVDLVQTIAEIGGAKAPTDWDGDSM
                     CSWMDDPQTEWKDLAVSEYYAHNIASGYAMLRTGGYKYVYHTPADEKHPAERELYDLK
                     ADPGELRNLAPDPAMQTRIREMHTRLVKELGEDPDRTELRARASHDTRVSTHSRGRPN
                     RENKQPPETALNR"
     gene            7137..>7577
                     /locus_tag="P5316_18690"
     CDS             7137..>7577
                     /locus_tag="P5316_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRR87043.1"
                     /translation="MLTDNILNLIGNTPLLRLKGENIYAKAEFLNPGGSIKDRVALAM
                     LEGAERDGQLKRDSIIVEPTSGNTGIGVALVGRLKGYKVRIVMPEGMSEERKKLIKAL
                     GAELILTPDKESIGGAVRRVAEMAAEDARVFVPQQFENPENPRIH"
BASE COUNT         1448 a         2196 c         2439 g         1494 t
ORIGIN      
        1 cacctcgatg gaccatcacc ggcgtgcctt ggcggcgcgc ggcgatcttg ttgtgtctga
       61 gggcccgctg ctcgtcggac tcgggcggga tctggttgat atagccgaga accggtttgc
      121 cctcagcttt gtcatccggg gcggcctgcg gtttctcggc aggcttcggc ggggccggtt
      181 tcttcactgt aacggccatt ccctgatcac tgaccttgag gaacagggtc tcgagatccg
      241 ggttcttcgt gaccgcctcg atgtattcct tcatttgtga accaagattc gtggtgttgt
      301 gagccaggat cagcccgttg ggccggacca gcggcaagag cttgttgagg taatcaaagt
      361 agccgggttt ttcggcgtcg atgaagacca tgtcgatcgg tcccttgagc cgtgagatct
      421 ccttatgggc atcgccttcg accagcgtga cgatggactc gacgccggcc cgcttgaagt
      481 tctcgcgggc caaggccgcc cgaccggcgt cgatttcgaa agtggtgagc ttgccgccgg
      541 tgctttgcag cgccagacag atccagaggc ccgaatagcc gttcgaggtg ccgatctcga
      601 cgacatgttt ggcgttggcg gccaaggcca tgaatcgaag caggcgggcg tcttcgatgg
      661 ggacgttcat catccctgcg cgcttggtgt cgagatcctt gagcacctcg gcaatccggg
      721 cttcatcggc gcggatgggg aagggctgtg acgtgtcttg agctttcagc gccgaggtgc
      781 cgagcaagca acccacggcg gcaatgatga tcaaccgacc ggcgcaattg gtccgattca
      841 tggttttctc cgattgagta gcaggatccg gcccccgacc gcgttgctgg gtggccgtcg
      901 gtcatcatca tatcgtttcg cgacaagacc gcgagttcga gggtcttgga tccgtcaggc
      961 aggacaaccg ataccgggca aagggggatg ggatattgac taagtatctg tccttaaaag
     1021 ggttaagtct ctccgggtat tcgcctgtgg ttgcctgacg gcaccgttcc ctttgctgag
     1081 acggcggtgg cagcgttggc tttcgcgaag tctgcgccgc agcaagggag ctgtaggtgg
     1141 cacttggtcg ggcaagaagg accggcagag gcgatccgcc agtggcatcc cgtgcttgcg
     1201 cttggggttc cgcaagagct accccaatcg acatcgaact ggcctgcggg gcatttacaa
     1261 gggtggccgt gccgactata cttgcaggat cggggtaggt ggcgtacgag aagagggttg
     1321 cgaacgtttg aggaagttca gccgagaagg aaaggatcag tttccgtatg attaaggtga
     1381 aggcccgtgg caacgaatcc ctggagcaga tgctccgccg gttcaagaag atgtgcgaga
     1441 aggaaggcct caccaaggaa atcaaacgga cggcctacta tgagaagccc agcgagcgcc
     1501 ggcggcggcg gatccgcaag tcggtgaagc gagtcgagcg acctatgggt cggtgagagc
     1561 atcgagagcg gcttcaggac tgcacggcaa acgggccaac aggcgaagtg ccccgggtgg
     1621 cagagctcgg ggtggttcgt ttttacggct ctttcatcct gccgcaccgc gcgcgagaaa
     1681 gtgtttcagt tcacatgcgg cctcggccgc cacgtcgatg gtcttgtcaa tgtcctcctc
     1741 ggtatgagcc agcgacagga accacggatg cggtgttgcg aacacgcctc ggcgctggat
     1801 cgccgcacaa aactgcttga ggacttcgtc gcgaagctcc tttgggcagg gggtcggatc
     1861 ggtttcgtcg gcgggcttga gcacgggcat gggggcgaat ccgacgaact tgatcggcag
     1921 ctcttgttcg cgagcgagac ggttgagccc gttcatgagc cgccggccga gccgccagaa
     1981 gtggtcaatg ccgtcccgtt ctttcagaac gcggatggtt gtcagcgcag ccacgacgga
     2041 caacaagtcg ccgtgaaagg ttgcggccat gtgggtgtcg gttcgctttt cgaggatgct
     2101 ccgctttcca acgaccacgg cggccggata gccgttgcac atggcttttc cgaaggttgc
     2161 gagatcgggc tcggcgccga accgcgggcc tgcgccctcc aggccgaagc ggaagccggt
     2221 cttgatttcg tcgaggatga agacggcgcc gtgctcatcg cagagctgcc gcacaccggg
     2281 gaagtaatcg ggcgaggggc cgttgtcctg taccgattcg accagcacgg cggcgacctg
     2341 gcccggcgcg gtttcgagca tattgcgcag ggccggaaga tcgttgtacg gaacggcgac
     2401 gctcagctcc cggttaaacc tgggaacgcc gggattgtcc ggcacgcacc agtcgagcca
     2461 gccgtgataa ccgcatcgaa tgatccgttc acgaccggtg tgagccctcg cgcagcggat
     2521 ggcgcccatg gtggccgcgg acccgcccac gaagaacatg accatctcgg cgcaggggat
     2581 ggtctggata agcatctcgg ccagttcgat ctcgagcgga tgttcgatgc tgaagaccgt
     2641 gccctcctcc atctgccgcc gcactgcttg gttaacttcg gggtcgcagt gtccgaggac
     2701 aatcgggccg tagcccagca ggtagtcgag gtactcgttg ccatcgacgt cccagaaccg
     2761 ggcccccttg cctctggcgg cgaacgaggg cccgccgagt ttcatgatgt ccacgggcct
     2821 cttgtgggcc tgcccgcccc cgacgatgca ttggcaggat cgctcccaaa gacgtcggga
     2881 ttctttgttg cgagtctcga tcaagttcga atcggacatt tcggccaccc ctgatgaaac
     2941 taccacctga ctgactgctg aattgtcgtg atcgtaacca gtgccgtgcc cgttggccat
     3001 cgcgatggag agttggcggg gcggcgcggg gttgttgtcc gcggccttgc ccgccggttc
     3061 gaggacgata tagtgtttcg ttccacaccc gccggacgcg gttcagcggc cgaggccctg
     3121 gcccgaccgg cggaaagcag acctgccctg gagagctagc gtgctgcgca aagaacccat
     3181 accgaccgct ccggaattca ctgtattgga gagaatccgc gtccacattt gtgctgatga
     3241 cgccgaggcc gcgcgagagg tcacgagaat cattgccgac ctgattcgcc agaacaatgc
     3301 gaacagccgc aagacggttc tcgggctggc gaccggcaac acgccgatca acgtctaccg
     3361 ggagctgatt cgactgcacc agcaggaaag ccttgatttc tcgcgagtcg tgacctttaa
     3421 cctcgatgag tattacccta tgaggccgga cagcattcag agttaccggc gctggatgca
     3481 ggaaaacctg ttcgaccaca tcaacgtcaa accggaaaac atccacatcc ctgacggctc
     3541 aattcccgag tcggaggtgt cggcctattg tgagcgatac gaggagatga tcgaacaggc
     3601 aggcggaatc gatattcaga ttctgggcat tggccgcacc ggtcatatcg gcttcaacga
     3661 gcccggctcg ctgatcgact cgcggacgcg gcttatcgat cttgacgccg tgacccgccg
     3721 ggatgccgcg gcggactttt tcggcgaggc gaacgtaccg caccgagcga ttaccatggg
     3781 cgtggggacg atcctgaaag cccggcggat tctgatgctg gccttcggcg agcaaaaggc
     3841 cccgatcatc cgccggaccg tcgaagggcc ggtcacgcac gccgttccgg ccacgttcct
     3901 acagaaccat ccgaacgccg agatctttat cgaccggggg gctgccggcg atctgacgcg
     3961 ggttgaatcg ccgtggttgc tcggggaaat cgaatggacg ccggagaatc agcttcgcgc
     4021 ggtgatctgg ctcagcctga agaccggcaa gagcattctg aagctggatc cggaggacta
     4081 cagcgggaat catctttcct cgcttgtgcg ggcggtcggt tcggccgacc ggctcaacca
     4141 tcggactcat cgacggctct cggcaacgat cgtcaccaag aatctgatgg cgacgaagcg
     4201 gaggatgctc tgtttcagcc cgcacccgga tgacgacgtg atcagcatgg gcggcacgtt
     4261 gacccggctc gtgcaaaggg agaacgaggt tcatgtggct tacatgacca acggcagcat
     4321 agccgtggcc gacgatcacg cactgcaatt gctggacttc tggtcggacc tgaatcacac
     4381 cttcaagctc gacaccgagt cgaccgcgat gttctatgac aaggtgcaca gatttctggc
     4441 ggacaaagag ccgggcgaag tggacagctt cgaggtacag gaaatcaaga ggctgattcg
     4501 cgagtctgag gctcttgcgg cgtgccggtc gatgggcata ccgacgcaga acgcccattt
     4561 tctcgacctg ccgttctacc agaccggtga agtgcggaag cgtcccatca gcgagcagga
     4621 tatcgagatt gttgccgggt tgatgcgtgc catacgccct gactgggtgt ttgtggccgg
     4681 cgagctgtcg gatccgcacg gaacgcaccg gttgtgtgcg gaggccattt tcgcggccct
     4741 gaggcggctg agcccggagg agaagccggt gcaggtctgg ctgtatcgcg gggcatggca
     4801 ggagtgggag ccggacgtga tcgacgtggt gataccgttt tcgcgaacgg agttgatgca
     4861 caagattttc gcgatcttca agcaccagtc gcagaaggac aaggcccttt ttcccggccc
     4921 gtatgacgat cgcgagttct ggcagcgggc cgaggcgcgg aacaccgaga cggcggcgat
     4981 tttcaacaag ctcggcctgc cggagtacta tgcgatggaa gccttcgtcc ggtacgatga
     5041 attctgagaa aagcggtccg ctctcaacga tcagctttgg aaagagaaag gcgggggcgc
     5101 tcaggctcgc gcctcacctg ttcagggcgg tttccggcgg ctgcttgttc tcgcggttgg
     5161 ggcgcccgcg actatgggtg cttacccttg tgtcgtggct tgcccgtgcg cggagttcgg
     5221 ttctgtcggg gtcctcgccc aattccttca caagccgggt atgcatttct cgaatccggg
     5281 tttgcatcgc cggatcaggc gcgagattgc gcagttcgcc gggatcggcc ttgaggtcgt
     5341 acaattcccg ctcggccgga tgtttttcgt cggcgggcgt atgatagacg tacttgtatc
     5401 ctccggtccg cagcatcgcg tatcctgagg cgatgttgtg agcgtagtac tcgctgaccg
     5461 ccaggtcctt ccactcggtc tgagggtcgt ccatccatga gcacatcgag tcgccgtccc
     5521 agtcagtcgg ggccttggcg ccgccgatct cggcgatggt ctgcacgagg tcgaccagtg
     5581 agcaggcctt gtcgcgtttc tggcctccgg gccagcgctt cggccatgag atgatcagcg
     5641 gtacgcgggc cgctgaatcg aacatgcagt tcttccacca caggccgtgt tcccccaggt
     5701 tctccccgtg atcggtggtg tagatgatca ctgtgtcctc ggccagtccg cttgcctcca
     5761 gagccgtcag caccttgccg atttccccat ccagccattg ggtgaggccg tagtacagct
     5821 cgcgtccgcg acggacggta tcctccggca ggccgatcag ctcgaagccg acgcggaggt
     5881 gacggtagtt gcgaggcagc gtgtcgaggt agccgggcgg aatctcgggc atgggtacct
     5941 tgtctctgta aggttgccaa tagcgggcgg gcacaatcag cgggaaatgc ggagccagat
     6001 agccgacgag caggaaaaag ggcttgtcgg tttttttccg gccggcgagg aagcgggtga
     6061 ctccggccgt gacctcgcgg tcatggttaa ggggccccga gctgtcaccc ggacggaact
     6121 gatcgaagcg tccggagatc gttccggacg gtgcgagatc atccggcctt cgacgcccca
     6181 gagcgccgct cttggttccc cgattggtgt cggccttgcc aagctcggca aaaccgtagc
     6241 ggtgctcgga gtcgtagtgc atcttgccgc acaagaaaga ctcatagccg gccgcattga
     6301 gaatgtgcgg aagcgagggc gtgtcatcag gcggaagtct ggcgctgttg ctccagacgt
     6361 cgatatgact gatgtacttg ccgagggtga atgacaggcg agccggcgca catagcggcg
     6421 acgtcgtgta ggcggcgtcg aacaaaacgc ctcgttcggc caaccggtcg agattcggcg
     6481 tatgggcgat cctgttgccg gcgcagccca tcaccgaggc gttgtgctcg tcggacatga
     6541 tcaccagtat gttcggctgc cggacccgcc gcgcgtccgt ttgaccgaat gtcggcgaca
     6601 ccacaacggc taacgccgca accgcgagcg ccaccgtcag ccctgtccat ctcgtttgcc
     6661 gatgtccctg attcgccatg acgtaccctc ccggttgcat gaaagcgtcg aatactaccc
     6721 tgcaccgcac tcaagattac taggttactt ccatgcccgg ccttcaaaaa cacaagattg
     6781 tttccatgcg cagcacttaa aaatgcgcgg ttatcaccca gcgcaacact tgagaatcca
     6841 aggttagcta ttcacagatc atgatctaca ttgacactga cgatacggtc cggcggtatc
     6901 ggtcgcagca actcattaag cacaaatggc ttatagtaat tcccaggaac ccgcggcccg
     6961 gctcaggcct ccgggtcggt attctgcccg ataccttggc catttatcgg tttcagattg
     7021 cacgaccacg cccgccggcg ggtcatccgg ctctcgcccc agtcgcaccg cgcctccctc
     7081 gtgctcggtc gggcggagtc ggatatgctt agccctggca aggagaaaaa cacgccatgc
     7141 tgaccgacaa catcctgaat ctgatcggca acacccccct gctgcgtctg aagggagaga
     7201 atatctacgc caaagcggag tttctgaacc ccggcggttc aatcaaggat cgggtggccc
     7261 tggccatgct ggaaggagcc gagcgggacg gccagctcaa acgggattcg atcattgtcg
     7321 agccgacctc cggcaacacc ggaatcggcg tggccctggt ggggcggctc aagggctaca
     7381 aggtccggat cgtcatgccg gaaggcatga gcgaggagcg gaagaagctc atcaaggctc
     7441 tgggggcgga actgatcctg acgccggaca aggagagtat tggcggggcc gtgcgccgcg
     7501 tcgccgagat ggcggcggag gatgcgcggg tgttcgttcc gcagcagttc gagaaccctg
     7561 agaatccgcg aatccac
//
LOCUS       DAOTPW010000271         7553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8584_length_7553_cov_4.862763, whole genome shotgun sequence.
ACCESSION   DAOTPW010000271 DAOTPW010000000
VERSION     DAOTPW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8584_length_7553_cov_4.862763"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="P5316_18695"
     CDS             complement(<1..408)
                     /locus_tag="P5316_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87044.1"
                     /translation="MFKKNLRAWAALAVTAGLVAMMGQTCIPRPTGGPDVSVTASPTS
                     GTNSVDVTLTATVTGGAEPYTYAWTASPAATITGADTANATATVTATTTFTCTVTDDN
                     GRTDSASVTVTVTRAITVTASAPGTALGGETVNL"
     gene            complement(699..833)
                     /locus_tag="P5316_18700"
     CDS             complement(699..833)
                     /locus_tag="P5316_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87045.1"
                     /translation="MLMLVFGARDRINEKLQIKLINNRCNAFGEYDLRGKITGGALGD
                     "
     gene            complement(1393..2655)
                     /locus_tag="P5316_18705"
     CDS             complement(1393..2655)
                     /locus_tag="P5316_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRR87046.1"
                     /translation="MPSVCFYFQVHQPYRLRRYSVFDTDYHYFNDAKNEEVCRKVAAK
                     CYLPATRLILDLVRRYEGHFKVAYSLSGVILEQFERYAPEVLDVFQRLAETGCVEFLA
                     ETYYHTLSFLYSRDEFAEQVAMHQDRIKQLFGCRPQVFRNTELIYNNDLAAYVAQLGF
                     KGILCEGADHLLGYRSPNYVYRPPNTDKAALLLRNYRLSDDVAFRFSNRHWSEWPLTA
                     EKFARWINQVNGQGHTVNLFMDYETLGEHQWAETGIFEFLNHLPHEIFKCRENNFKTP
                     SEVIDTYPVSGVYDVPHMISWADTERDLSAWLGNAMQSNALHELYRLERDVKACGDPN
                     ILRDWRIMQGSDHFYYMCVKYFADGDVHRYFNPFDSPYDSYINFMNALDNLRSRLKVK
                     QRVVRALTFPPGRRVREGQPSRSPEYTG"
     gene            complement(2955..>4066)
                     /locus_tag="P5316_18710"
     CDS             complement(2955..>4066)
                     /locus_tag="P5316_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87047.1"
                     /translation="GGPGDDTMTGGLGEDTASYACDIFSIPCVDPTEFTVSQDPDTGV
                     YFVPNDGQGGTDLLPGIEQVLGPAGPIDLDAGTGGGGGGGGGTVPAPQLQIILGVQGE
                     LKVTEPGGQVQLQFSANGGVPPYTAEWDPTTDIDPANSKRKVTILTYAGGSFLAGQTQ
                     TAIASPVRDTSYRVTVTDSKGAVRVAFVKVKVAAEFSVFAGADRTISLGQQITLRPTV
                     TGGVAPYTYSWQVQGTGDGGFLTAKNIPAVTVAPTETTTYVLTVTDSIGSTATDAVVV
                     TVLGTNGIPVTPGGDTGGEPTEPGSTEPGGNGENPGNNGGGQSGGGPSGGDSVDDSAS
                     GSPVRWSLPICGAGANLTLVMAGLFMLAVMKRREW"
     assembly_gap    4067..4166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4167..>7553)
                     /locus_tag="P5316_18715"
     CDS             complement(<4167..>7553)
                     /locus_tag="P5316_18715"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRR87048.1"
                     /translation="GNDTMESGDGNDVVFGDGLNPNDDQISGNDQIRLGDGNDVAFGG
                     PGDDFIDCGPGDDGTLEQPVEGNAGSDIILGGPGNDWIDAGADDDGNRGDDMPVDGGP
                     GNDTIWGGDGNDYISGGDESPAECLLAPKNRELGVPGLGDFFCDVIFGGPGDDLLKGG
                     DGNDFIEGGPGNDDINGQWGYDYVFGGDGDDTVSGGGDNDIITGGPGNDTLSGGGGEF
                     DLLDYLRDGGDGGVNVNLPAGTATDTWGDEDTLGAFSAVRTTNLPDRIIGNADRATVV
                     LALGGNDYIMGGRRADILMGNTGDDTIHACGAQSDGGDEASSCLNGDWVADVDLVLGG
                     SGNDDIYGYTSVDYLFGDGDELLGLGPTALTPFDTNGNRIPDFELGEPNPFDPTMPQN
                     IEILSFRLIVERVLRDPDTNEYTQMVPPTSSAFAIGADRIWGGPGDDVVVGGAGGDEL
                     YGDGRYEESPGSGTYVDTSGRDFIWGDLHVPLGKPPFFPDTLAAGNDTIAGGPRVDVI
                     YGCGGHDTIYGDLENGRETAGTGFWGESIAWDDIIYGDYDYDYLDVDGFPLSRYWDDG
                     IWEIDPTAPATLVIGDDTIYGQEGDDEIYGGAGNDYLVGGPEGDIIQGNAGVDRIFGG
                     NDKVSGYGYQDFSFRDTVDYSSASQWYHSGVVVWLGGSGARRDTLGWAYDDGDPIRSG
                     SSTGYDYIYGIENVLGSDFDDVIIGTGETLSEVAGSASEYNWVNCYKDYYANRVDTYY
                     MTYYGDYVNILVGRLGNDIIMGRGGEDLLLGGVGNDIICGDADMHCDPGRTDDPDIVA
                     IIGDKLAPNLNLKAEDAIGGDRDYLFGEYGNDKLYGDGGDDIIRGGPGADVLSGGLGI
                     DVLDYSDFVPSVSAGSPQAVTPVVVNLRDTPVDLATFEEELMDAADLAVLPALPTSQA
                     PLPLAPCSNLNTVICPNMAVDGGDWGLSGAIPPIPATPRSVDTIINSPAGDPLDRLEI
                     VLGTNGDDVMFGHPSKPNILVGWSGNDILVSGNSVYDPNQRDPDLEGNRLRNAASPIL
                     YDNLIFGDGLGTGDAASELALQLLPTDLGTLPRAGDDVIESGKGDDYLNGGNQTDLGV
                     GDFVSYSHAQGPVHVEMSATYPQNTVSAGWDTLAGFENLIG"
BASE COUNT         1444 a         2554 c         2061 g         1394 t
ORIGIN      
        1 caggttgacc gtctcgccgc ccaacgccgt cccgggcgcg ctggccgtca cggtaatcgc
       61 ccgcgtcacc gtcacggtca cactggcact gtcggtcctg ccgttgtcgt cggtcaccgt
      121 gcatgtaaag gtggtggtcg ccgtcacggt tgccgtggcg ttggccgtgt cggccccggt
      181 gatcgtggcc gccggactgg ccgtccaggc gtaagtgtac ggctcggcgc cgccggtgac
      241 ggttgccgtc agcgtgacgt cgacgctgtt ggtgccggac gtcggtgaag ccgtgaccga
      301 gacatccggg cccccggtag gtctcggaat gcacgtctgg cccatcatgg cgacaagtcc
      361 agccgttacc gccagagccg cccaagccct taggtttttc ttaaacattc tgcatacctc
      421 ctgcactaga actaagaacg ctacaattct gtttcttcga aggaacacga ctcagaccga
      481 tgcccggcct ggtttcacct gtctcgccgc ccgccagacg ggctccgccc gcgggtttcc
      541 agacctcgga ttgtgcgacc cgggccctgg aaagccgcgg ccgctaccag cgaaacagga
      601 tggccacacg accgaccaaa ggccttcgcc gaccatcccc tcgaaacgat taccagattg
      661 tcaaacatcg tctccggcgg accgcccgcc ggcttatatc agtcccccaa cgcgccgccg
      721 gtgatctttc cccgcaggtc atactcaccg aacgcgttgc atcgattgtt aatcaatttg
      781 atttgcaact tctcgttaat gcgatctctc gcgccaaaga ccagcatcaa catctactta
      841 cgtcaccttc aggccttcgg acgcgccagg taaatcggca cgaaaaaccc aagcccgata
      901 aagaacgccg acgcccaatc ttacggaccc accgagggtt gtcaataccg cctgtccact
      961 tttttcagcc gccatgtccc ctttgcggaa aagcccgcgc cgccatacct ggcacccaac
     1021 aggcccacgc caacctcagc accggatcgc ctccctgcgg aggtaccctc caacgccccc
     1081 atccgtgcgg acgtactgat gccttgtcat cgtttatcgc ccgaatccgc cccaatacaa
     1141 cagtgttttc cccaaattaa cccaaaaaca gccctctgcc agtacgcagg acaaggttcg
     1201 gcagtgggct tgcggcagcg ccgcatgcga caagactacg cctgaacacc cagactcaaa
     1261 agcccagcgc cacggagcat cacctgaaga cgggttgccg tcagatcgtc cgggtgtctc
     1321 atcgtggttt gtatgcatcc ccgctcgtcg cacgcctaca ccatgggtca tccaggctcg
     1381 gcgatgctcc ggctatcccg tgtactccgg ggacctcgac ggctggccct cgcgaacgcg
     1441 acgcccgggc ggaaacgtca gcgccctgac tacccgctgc ttgaccttca gacgcgagcg
     1501 gagattgtcc aacgcattca tgaagttgat gtaagagtcg tacggcgagt cgaaaggatt
     1561 gaaataccga tgcacgtcgc cgtcagcaaa atactttacg cacatgtaat agaaatggtc
     1621 cgaaccttgc atgatccgcc agtcgcggag aatgttcgga tcgccgcaag ccttcacgtc
     1681 cctctccagc cggtacagct cgtgcagcgc atttgactgc atggcgttgc ccagccacgc
     1741 cgacagatct cgctccgtgt ccgcccaact gatcatgtgc ggcacgtcat acaccccgga
     1801 gaccggatag gtgtcaatca cctccgaggg cgtcttgaag ttgttctccc ggcacttgaa
     1861 gatctcgtgg ggcaggtggt tgaggaactc gaagatcccc gtctcggccc actggtgctc
     1921 gcccagcgtc tcatagtcca tgaacaggtt gacggtgtgc ccctggccgt tgacctgatt
     1981 gatccagcgg gcgaacttct cggcggtcag cggccattcg gaccagtgcc ggttggagaa
     2041 acggaaagcc acgtcgtcgc tcagccggta attcctcagc agcagggccg ccttgtcggt
     2101 gttcggcgga cgatatacgt aattcggcga gcgataaccg agcagatggt cggccccttc
     2161 gcaaaggatg cccttgaagc ccaactgcgc cacgtatgcc gccagatcgt tgttgtaaat
     2221 cagctcggtg ttgcggaata cctgcggcct gcaaccgaaa agctgcttga tccggtcctg
     2281 gtgcatcgcc acctgctcgg cgaactcgtc cctcgaatac aggaaactca gcgtgtggta
     2341 gtaggtttcg gcaagaaact caacgcaacc ggtttccgcc agccgctgga aaacgtcgag
     2401 aacctcgggg gcgtatcgtt cgaactgttc gagaatcacc ccgctgagcg aataggcaac
     2461 cttgaaatgg ccctcgtagc gccgcaccag gtcaagaatc agccgcgtcg ccggcaggta
     2521 gcacttggcc gccaccttcc ggcagacctc ctcgttcttg gcatcgttaa agtaatgata
     2581 atcagtgtcg aaaacactgt aacgccggag tcggtacggc tgatgcacct gaaagtagaa
     2641 acacaccgat ggcactggca cacctctggc cgcagacggc cggaactgaa cgggacagtg
     2701 ctgcctcttc aagaaacgcc ccgcgggcag acaccttgtc ccgggaaccg caagcccccg
     2761 gcgcaatcgc cgccgctcga aaaccgctgc ttcatcgcca ccccctccgc gcggaggctg
     2821 attgtattat aactcactta agcaaacagg gacagtggac gcaacagctc atgcgcgaca
     2881 aaaaaactcg accgggcccc agggacccgg tcgagcatcg tttctttcct tcagacgctc
     2941 cgatgaaccg gccgttacca ctcccgccgc ttcatcaccg ccagcatgaa cagcccggcc
     3001 atcaccagcg tcagattcgc gcccgcccca cagatgggca aagaccagcg caccggcgag
     3061 cccgaagccg agtcatccac actgtcgccg ccgctcgggc cgccgccgct ctgaccaccg
     3121 ccgttgttcc ccgggttctc accgttgcca cccggttctg tgctgccggg ctccgtcggc
     3181 tccccgccgg tgtcgccacc gggcgttacc gggataccgt tggtgccgag gaccgtgacg
     3241 acaactgcat ccgtggccgt cgatccgatg ctgtccgtaa ccgtcaggac gtaagtcgtg
     3301 gtttcggtcg gagcaaccgt gaccgcagga atgttcttgg cggtcaggaa cccgccgtca
     3361 ccagtcccct gaacctgcca ggaatacgta tatggggcaa cgccgcctgt caccgtcggc
     3421 ctcagcgtaa tctgctggcc gagcgaaatg gtccggtccg cgccggcaaa cacgctgaac
     3481 tccgccgcga ccttcacctt cacgaaggcc actctgaccg ccccttttga gtccgtcacc
     3541 gtgacccggt acgaggtgtc gcgaaccggc gaggcgatgg ccgtctgcgt ctggcccgcc
     3601 aggaacgagc cgcccgcata agtcaggatg gtcaccttac gtttcgagtt cgcggggtcg
     3661 atgtccgtcg tcggatccca ctcggccgtg taaggcggaa caccgccgtt ggccgaaaat
     3721 tgcagttgga cctgcccgcc gggctccgtg accttcaact cgccctgaac ccccaggatg
     3781 atctggagct gcggagcagg cacggtcccg ccaccgccac cgccaccgcc gccagtgccg
     3841 gcatcgagat cgatcggacc ggccgggcca agtacctgtt cgatgcctgg caacaaatcc
     3901 gtaccgccct ggccgtcgtt ggggacgaag tacacgcccg tatcaggatc ctgcgatacg
     3961 gtgaactccg ttggatcgac acatggaatg ctgaagatgt cacatgcata ggaggccgtg
     4021 tcctcgccca gccccccggt catggtgtca tcgcccggac cgcccgnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnccga tcaggttctc gaaccccgcg agcgtatccc
     4201 agccggcact gacagtgttc tgcgggtacg tcgcactcat ttcgacatga accggcccct
     4261 gtgcatgcga gtaggaaacg aaatcgccca cgcccagatc ggtctggttg cctccgttca
     4321 ggtaatcgtc ccccttgccc gactcgatca cgtcgtcgcc cgctcttgga agggtaccca
     4381 gatccgtcgg cagcagttgc agcgcaagct cgctcgcagc gtcgccggtg cccaagccat
     4441 ctccgaagat aaggttgtcg tacaggatcg ggcttgcggc attccgcagc cggttccctt
     4501 cgagatcagg atcgcgctgg ttcggatcgt aaaccgaatt gccgctcacc aggatatcgt
     4561 tgccgctcca accgaccagg atgttgggct tcgacgggtg accgaacatg acatcgtcgc
     4621 cgttggtccc caggacgatc tcgaggcgat ccagaggatc gcccgctggc gagttgatga
     4681 tcgtgtccac cgaccgcggt gtcgccggga tcggcgggat ggcacccgaa agcccccaat
     4741 ctccgccgtc caccgccatg ttcggacata tcaccgtgtt caggttcgag cagggagcca
     4801 ggggcagcgg cgcctgggaa gtaggtagcg ccggcagaac cgccagatca gcagcatcca
     4861 tgagctcctc ttcaaacgtc gccaggtcca ccggcgtatc ccgcaggttc accaccaccg
     4921 gcgtgaccgc ctgaggtgag cccgctgaca ccgacgggac gaaatccgaa tagtcgagca
     4981 catcgatgcc caggccgccc gacagaacat ccgcgcccgg accgccgcgg atgatgtcat
     5041 cgccgccgtc gccgtaaagc ttgtcattgc cgtactcgcc gaacaggtag tcacgatcgc
     5101 ccccgatcgc gtcctcagcc ttgagattca gattcggcgc cagcttgtcc ccgatgatgg
     5161 ccacaatatc ggggtcgtcg gtcctgcccg gatcgcagtg catgtccgca tcgccgcaaa
     5221 tgatatcatt gcccacgccg ccgagcagca gatcctcgcc gccccggccc atgatgatgt
     5281 cattgcccag ccggccgacc aggatgttca cataatcacc gtaatacgtc atgtagtagg
     5341 tgtcgacgcg gttcgcgtaa tagtccttgt agcaattcac ccagttgtac tcagaagcgg
     5401 aaccggccac ctccgacagc gtttcgcctg tgccgatgat cacatcatcg aaatccgagc
     5461 ccagcacatt ctcgatgcca tagatgtagt cgtatccggt gctggagccc gacctgatcg
     5521 gatcgccgtc gtcgtaggcc cagccgaggg tgtccctgcg ggctccgctg ccgcccaacc
     5581 agaccaccac acccgaatgg taccactgcg acgcgctgga gtaatcgacc gtgtcgcgga
     5641 aactgaagtc ctggtagccg tacccggaaa ccttgtcgtt accgccgaag atccggtcaa
     5701 cgcccgcgtt gccctgaatg atgtcgccct cgggcccgcc caccaggtag tcgttgccgg
     5761 cgccgccgta gatttcgtcg tcaccctcct ggccgtatat ggtatcgtcg cctatcacca
     5821 gcgtagccgg agccgtggga tcgatctccc agatgccgtc atcccagtac cgcgagagcg
     5881 ggaagccgtc gacatcgaga tagtcgtagt cataatcacc gtagatgatg tcatcccacg
     5941 caatactctc gccccaaaag cccgtgcccg cggtctccct tccgttctcc agatcgccgt
     6001 agatggtgtc atgcccgccg caaccgtaga tcacatctac cctcgggccg ccggcgatgg
     6061 tatcgttccc ggctgcaagg gtatcaggga aaaacggcgg cttgcccagt ggaacgtgca
     6121 gatcccccca gataaagtcc cggccggaag tgtccacata ggtgccacta ccgggcgact
     6181 cctcatacct gccgtcgccg taaagctcgt cgccgccggc cccaccgacc acgacatcgt
     6241 ctccaggccc cccccagatt ctgtccgcgc cgatggcaaa ggcactgctg gtcggaggca
     6301 ccatttgagt gtactcgttg gtatcaggat cacgcagaac ccgttcaaca atcagccgga
     6361 agctcaagat ctcgatgttc tggggcatcg tcgggtcgaa ggggttcggc tcgcccagct
     6421 cgaagtccgg tatcctgttg ccgttggtgt cgaagggcgt cagggcggtg ggcccgaggc
     6481 cgagcaactc gtcgccgtcg ccgaaaaggt aatccacgct tgtatagccg tagatatcat
     6541 cgttgcccga cccgcctaac accaggtcta cgtctgccac ccagtcgccg ttcaggcagc
     6601 ttgacgcttc gtctccccca tcactctgtg ccccgcaggc gtgaatcgtg tcatcgcccg
     6661 tgttgcccat gagaatatcc gcccgccggc cgcccatgat gtaatcattg ccgcccaagg
     6721 ccagaacaac ggttgcccta tccgcgttgc cgatgatgcg atcgggaaga ttcgtggtgc
     6781 gaacggcact gaaggcccca agcgtgtcct cgtcgcccca ggtatcggtc gccgtgccgg
     6841 caggcaggtt cacattaacg ccgccgtcgc cgccgtcgcg caggtaatca agcaggtcga
     6901 attcaccccc tccgccggaa agcgtgtcat ttcccggccc gcccgtgatg atgtcattgt
     6961 cgccgccgcc ggagacagtg tcgtcaccgt cgccgccgaa cacatagtcg tatccccact
     7021 ggccattgat gtcatcattg ccggggccgc cctcaataaa atcattgccg tccccgcctt
     7081 taagcaggtc gtcgcccgga cctccgaaaa tcacgtcgca gaaaaagtcc ccgagcccgg
     7141 gcacgcccaa ctcgcggttc ttcggtgcca gcaggcattc cgcgggcgac tcatccccgc
     7201 cgctgatata gtcgttcccg tcaccccccc agatcgtgtc gttgcccggg ccgccgtcga
     7261 ccggcatgtc gtccccacgg ttgccgtcgt catcggcgcc cgcgtcgatc cagtcgttgc
     7321 ccggcccgcc caggataatg tcgctgccgg cgttaccctc gaccggctgc tccaaggtgc
     7381 catcgtcgcc ggggccgcaa tcaatgaaat catcgcccgg cccgccaaaa gccacatcgt
     7441 tcccatcgcc aagccggatc tggtcattgc cggaaatctg atcatcgttg ggattcagac
     7501 cgtcaccaaa caccacgtca ttaccgtcgc ccgactccat cgtatcgttg ccc
//
LOCUS       DAOTPW010000272         7437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8739_length_7437_cov_4.991601, whole genome shotgun sequence.
ACCESSION   DAOTPW010000272 DAOTPW010000000
VERSION     DAOTPW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8739_length_7437_cov_4.991601"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="P5316_18720"
     CDS             <1..290
                     /locus_tag="P5316_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRR87049.1"
                     /translation="LLTVTNSQDGYCYTIDIGEAVDGLASLDTDCAVQVLGGDVNGDG
                     GVSATDLAFVKSKVGQAIEGNEKLDLTCDGSITATDMAYLKTRVGRQATCW"
     gene            complement(378..1004)
                     /locus_tag="P5316_18725"
     CDS             complement(378..1004)
                     /locus_tag="P5316_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87050.1"
                     /translation="MHVLVDDRPYTMAGPANQSLAELANEACAANRQDEPRYVVSIRC
                     DGQPLSQEDLAEALESPAERYERVEIQTQPVAALVRSALDQAALVLEDSTIAREKAAD
                     LLAAGQHEAAMQELQKFLEAWKQIHQTLMVVSQVLGIDLDSVADDRARLSTVLEVVKS
                     RFAEMKDAMVQNDFVVVGDLLRYELSEPVEQLAGLLRGLGRSARPNPP"
     gene            complement(1205..3031)
                     /gene="glmS"
                     /locus_tag="P5316_18730"
     CDS             complement(1205..3031)
                     /gene="glmS"
                     /locus_tag="P5316_18730"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRR87051.1"
                     /translation="MCGIVGYIGRKQAQPLLIEGLKRLEYRGYDSAGIATVGGGTLNI
                     TRAAGRISALEAKLGAAEDSATLGIAHTRWATHGAPSERNAHPHTSSDGKIALVHNGI
                     IENYRSLRTFLEQQGIRCVSDTDTEVLAQLIGHFYNGNLEQAVRDALHDVQGTYGIAV
                     VSADEPNRIVVARKGSPLIIGIGNGEFIVASDAAAIIEHTAQVVYLADGEMAVLTYDG
                     FRTMTLDAVPVTKDVQEVELTLEQIELGGHKHFMAKEIFEQPQALSNTLRGRLRVREG
                     MIHLGGIAHYARDLARARRIIFTAQGTAWHAGLVGEYLFEDIAKTPAETEYASEFRYR
                     NPLINEGTVVIAISQSGETADTLAALREAKEKGALTLGVVNVVGSTIAREADAGVYLH
                     CGPEIGVASTKAFTSQLAVLTMMAIQLGRRRFLSQNDAQALISQLGLIPGHVERIVQQ
                     SDAICEAARRFVDRENWLFLGRGYNYPIALEGALKLKEISYIHAEGMPAAEMKHGPIA
                     LISDGMPVVFIATRGNQYDKVLGNIEEVRSRGGKILAVATEGDRNVAQMAEHVFQVPD
                     VPEPLQPMLTVIPLQLLAYHAAVLRNCSVDKPRNLAKSVTVE"
     gene            3399..3809
                     /locus_tag="P5316_18735"
     CDS             3399..3809
                     /locus_tag="P5316_18735"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRR87052.1"
                     /translation="MLAGHPYTPILILLLVVIVIVLAIMALSHMIGTARHGHRKDTPY
                     EAGMPPLADARRRFHARFYIVALLFLLFDVEVVLMWPWAVAFHRAAVGQGSIEAVSGQ
                     AAGAGLFLVSMMVFVALLLIGLLYAWGRGALHWR"
     gene            3824..4435
                     /gene="nuoB"
                     /locus_tag="P5316_18740"
     CDS             3824..4435
                     /gene="nuoB"
                     /locus_tag="P5316_18740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRR87053.1"
                     /translation="MNARAAARRSVEALVSGEWVTTRLEAVSNLIRRYGVNWCRKYSL
                     WPLPFATACCGIELMATGASRYDVARFGAEVMRFSPRQSDLLICAGRVAIKMMPVLQR
                     IYAQMCEPKWVISMGACASTGGVFDTYAVVQGIDHFMPVDVYVPGCPPRPETLIEGIM
                     AIQRIVDREGIRNSQDRQGGYRMTVSASGKDQPRGDRSRQAGE"
     gene            4439..4969
                     /locus_tag="P5316_18745"
     CDS             4439..4969
                     /locus_tag="P5316_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRR87054.1"
                     /translation="MAIRSVISALQEQFPGVDFRPGPLLPGRSNELRQICLHLPSDRL
                     IEIMTFLRENEMTRFEQLSDLTCVDYLRYPGAANRFAVVYSLLSISLGHRLWIKCLVD
                     DPAPTLPSVTGIWPGADWLEREVYDLFGISFTGHPDLRRLMTWEGFTAHPLRKDYPLR
                     GQGERQNYEVFPRDVS"
     gene            5008..6177
                     /locus_tag="P5316_18750"
     CDS             5008..6177
                     /locus_tag="P5316_18750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR87055.1"
                     /translation="MAVSCRPDRRIRLTYVITDLAIGGVPLHLYRLATRLPRNRFHIH
                     VVSLADAGPVGEMLASAGIPVSSCGARTSRDLKALGRLWRILKATRPQVLHALLFHAN
                     IACRLIGPLAAVPINRILCEIQTAEVERPWHLVLDNLTCRLCRFEVGNSPSVVAHLRR
                     KAHLPASRLLCQWGAVDVAAIDAAKPADRAMFGLSADKPLVIWTGRFDPIKGFEEMLS
                     AFARLRQTLPATLLLVGDGPYRPTVERLIAEQGLDQCVKLAGSRPDVPSLLKIADAFV
                     FCSRTEGLPNSLLEAMAAGLPVVATDVPGCRDVVIDGKTGFLARAGSVVDIEKRLARV
                     LTDHRLSADLGTQARDWVRTHVDARKWSERWQSIYSYIAQNDSITVRADCLSRHG"
     gene            6335..7021
                     /locus_tag="P5316_18755"
     CDS             6335..7021
                     /locus_tag="P5316_18755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR87056.1"
                     /translation="MSQISDAKPLTEKPITVVVVDDHGIVRDGVSGMLSREKDIRVVG
                     MAENADEAISKVVETRPDVVLMDIDMPGVSCFDAVQIIRSRVPNTKVILVTAHEHDEY
                     IEQALRVKANGYLIKTQSMAALADAIRDVANGKIHFSPEVMDRLVVKGGKIELENPPK
                     TRRSMLSPRERELLRILAKGVSLKEAASILGISYKTADKQKASLMAKLDIHNRVELSR
                     FAIREGMIEP"
BASE COUNT         1391 a         2214 c         2215 g         1617 t
ORIGIN      
        1 tcttgctgac tgtgactaac agccaagacg gttactgtta caccattgac atcggtgagg
       61 cggtcgatgg tctggcttca ctggatacgg attgtgccgt ccaggtgctc ggtggtgatg
      121 ttaacggtga tggcggcgtt agtgccacag acttggcctt tgtgaaaagc aaggtgggcc
      181 aggcaatcga gggcaatgaa aaactggatt tgacttgtga cggctcaatt acagcaaccg
      241 acatggcgta tctgaagacc cgggtaggca gacaggccac ctgctggtaa ttgcgtttgt
      301 taaccgcaag caagcattgt gttttagatg aagccagtgg cgccatggtg gcctgctggc
      361 ttccttgcgt tatgctttta tggtggattg gggcgtgccg agcggcccag gcccctcaac
      421 aaaccggcca gttgctccac cggttcgctc aactcatatc gcagcaggtc gcccaccacg
      481 acaaagtcgt tctgaaccat ggcgtccttc atctccgcga aacggctctt aaccacctca
      541 agaaccgtgc tgagcctggc cctgtcgtcg gcaacactgt ccagatcgat tcccagcacc
      601 tgtgaaacga ccatcagtgt ctggtggatc tgcttccagg cctccaggaa cttctggagc
      661 tcctgcatgg ccgcctcatg ctggccggct gcaagcaggt ccgcagcttt ctctctcgca
      721 atcgtggaat cctccagcac caacgccgcc tggtcaaggg cgctgcgaac cagcgccgcc
      781 accggctgtg tttgaatctc cacacgctcg taccgctccg cgggagactc aagtgcctcc
      841 gccagatcct cttggctcag gggctgtccg tcgcatcgga tactgaccac gtatctgggt
      901 tcgtcctgac ggttggccgc gcaggcttcg ttggccaact cggcgagact ctgatttgct
      961 ggcccggcca ttgtgtacgg ccggtcgtca acgagtacgt gcatggcggt cctttcaagt
     1021 gttccgtctg gcggtcggtg tgcccgcagg cagcggcaag ctcgcctatt aaggcatcgg
     1081 catgttggcg tttgccgctc cagccggcct cctttttctc ggggatcttt cgttggtgac
     1141 cggtggcggg agagcataag gaggacagcg agttacgtcc cgctgatctt cgtcagccag
     1201 ccttctattc cacagtgacg ctcttggcca gattcctcgg cttgtccacg ctgcagttgc
     1261 gaagcactgc cgcatggtag gccaacaact gaagcgggat cacggtcagc atgggctgga
     1321 gcggctccgg tacatccggc acctggaaca cgtgctcggc catctgggca acgttacgat
     1381 cgccttccgt cgctacggcg aggatcttgc cgccccggct gcgaacctct tcgatgttgc
     1441 ccagaacctt gtcgtactgg ttcccgcgcg tcgcgataaa gaccacgggc atgccgtcgc
     1501 tgatcaacgc aatcggaccg tgcttcatct cagcggcagg cattccttca gcatgaatgt
     1561 agcttatctc tttaagcttc agggcacctt ccagggcgat cgggtagtta tagccccgcc
     1621 ccaggaacag ccagttctcc cggtcgacga accgccttgc ggcctcgcat atggcgtccg
     1681 actgctggac aatccgctca acatgaccgg gtatcagccc aagttggctg atgagtgcct
     1741 gggcgtcgtt ctgagacaga aaacgacgtc gaccaagttg aattgccatc atggtcagca
     1801 cagccagttg ggaggtgaac gccttggtgc tggccacgcc gatctcagga ccacaatgaa
     1861 gataaacgcc ggcgtcagcc tcgcgggcaa tcgtgcttcc gacgacgttc accacgccga
     1921 gcgtcagggc ccccttctct ttggcctcgc gaagcgctgc aagcgtgtcg gcggtctctc
     1981 cggactgact gatggcaata accaccgttc cctcattgat cagcggatta cggtaacgaa
     2041 actcgctggc atactcggtc tcggcagggg tcttggcgat gtcctcgaaa agatactccc
     2101 cgacaagacc cgcatgccac gcggttccct gggcggtgaa aatgatccgt cttgctctgg
     2161 caagatcgcg ggcatagtgc gcaatgcccc ccagatgtat catgccctcg cggactcgca
     2221 gccttcctcg taaggtgttg ctgagcgcct gcggttgctc gaagatctcc ttggccatga
     2281 aatgcttatg cccgcccagt tcgatctgct cgagcgtcaa ttcgacttcc tgcacgtcct
     2341 tcgtgaccgg aaccgcatcg agtgtcatgg ttcggaagcc gtcgtaagtg aggactgcca
     2401 tctcgccgtc agcgaggtag actacttgag cggtgtgctc gatgatggcg gccgcatccg
     2461 aggcgacgat gaactcgcca ttcccgatgc cgatgatcaa cggactgcct ttacgagcta
     2521 cgacgatccg attcggctca tcggccgaga caacggcgat cccgtacgtg ccttgcacgt
     2581 cgtgaagggc gtcacggacg gcttgttcga ggttgccatt gtagaagtgg ccgatcaatt
     2641 gcgctaacac ctccgtatcc gtgtcgctga cgcaacgaat gccttgttgc tcaagaaacg
     2701 ttctcagaga gcggtaattc tcgatgatgc cattgtggac cagggcgatc ttgccatcgg
     2761 aagaggtgtg cgggtgggca ttccgctcac tgggagcccc gtgtgtggcc cagcgggtgt
     2821 gggcgatgcc aagtgtcgcc gagtcttcag ccgcccccag cttggcctca agggcactga
     2881 tgcgcccggc cgcccgggtg atgttcagag tgccgccgcc taccgtcgca atgccggccg
     2941 agtcataacc acggtactca agacgcttaa gcccctcaat caggagcggc tgggcctgct
     3001 tgcgtccgat ataaccgacg attccgcaca tggtcgtatc ctcttgtcct ctcgatttgc
     3061 ttaatcggat ccggcgagga ctgcaagttc atcgaccgcc gcgttgcgac agtcaattag
     3121 tgcgtggcgc cgggcctgca atcctacggc cagacagatg cgcagcatgc gcgcatgtgt
     3181 ccgaattgct gtgtgtccgg gtcgcggccc gcaaaaaggc ggttcacctt gggattctcg
     3241 ggtgccgcgg attgcatgaa ccaccgtagc cgcttgatgt tacggaaatg acactttaga
     3301 ctgccaagac gcctataata ccgccgagcg aaagactcgg tccggtgtga agcaagacgg
     3361 cgtcgcaaga ttgctggcaa cccgaaagac aggcagccat gttggccggg catccgtaca
     3421 ctccgattct catcctgctc cttgtggtga tcgtcatcgt gctggccatc atggccttga
     3481 gccacatgat cggtaccgct cgtcacggtc atcgcaagga taccccctac gaagccggaa
     3541 tgccgcctct agccgatgct cgccgccggt ttcacgcccg cttctacatc gtggcgttgc
     3601 tcttcctgct ttttgatgtt gaggtggtgc tcatgtggcc ttgggctgtg gctttccacc
     3661 gggcggcagt tgggcaggga tcaatcgagg ccgtatcggg gcaagcggcg ggtgcaggtc
     3721 ttttcctcgt atcgatgatg gttttcgtgg ctctgctctt aatcggcctc ctttacgcgt
     3781 gggggcgggg agcacttcat tggaggtagg caaccagacc cccatgaacg cgcgcgccgc
     3841 agccagacga tcggttgagg ctctcgtgtc cggcgagtgg gtgacgacgc gcctggaggc
     3901 ggtgtcgaac ctgatccgcc ggtacggagt caattggtgc cgcaagtact ccctctggcc
     3961 tttgcccttc gcgacggcat gctgcggaat cgaactgatg gcgacggggg cgagccgcta
     4021 tgacgtggcg aggttcggag cagaggtcat gcgattcagc ccccgccagt cggatctgct
     4081 catctgtgcc ggccgtgtcg cgatcaaaat gatgcccgtc ttgcagcgaa tttacgccca
     4141 gatgtgcgag cccaaatggg tcatcagcat gggagcatgc gcaagcaccg gaggcgtctt
     4201 cgatacgtac gccgtcgtcc agggcatcga tcactttatg cccgtcgatg tctatgtgcc
     4261 cggctgcccc ccgcgtcccg agaccctgat cgaagggatt atggcgattc agcggatcgt
     4321 cgatcgcgag ggaattcgta acagccagga tcgacaaggc ggctatcgga tgacagtctc
     4381 cgcatccggg aaagatcagc cccgcggcga tcgctctcga caggcaggag aatgaccgat
     4441 ggccattcga tcggtgattt ccgcgttaca ggaacagttt cccggcgtgg atttccgtcc
     4501 gggcccgctt ctgcccggac gatcaaacga gctccggcag atctgccttc accttccttc
     4561 cgaccggctg atcgagatca tgacgtttct ccgtgagaac gagatgacgc gattcgagca
     4621 gctttccgat ctcacctgcg tagactacct tcggtacccc ggcgccgcaa atcggttcgc
     4681 ggtcgtttac tctttgcttt cgatatcgct tgggcatcgt ctgtggatca aatgcctggt
     4741 tgacgatccc gctccaacgt tgccgagcgt tacgggcatc tggccgggag ccgactggct
     4801 tgagcgggag gtgtatgacc tgttcggaat cagcttcacc ggacatcccg acctgcgacg
     4861 tctcatgacc tgggaaggct ttacggctca cccgcttcgc aaggattatc cgcttcgagg
     4921 gcagggagag cgtcagaatt acgaggtctt tccacgagac gtcagttgat ctgcactgtc
     4981 gtcccttccg aaaggcggat gattgaaatg gcggtatcgt gcaggccgga tcggcgcatt
     5041 cggcttacat atgtgatcac ggatctggcg attggggggg tcccccttca cctgtaccgg
     5101 ctcgcaaccc ggctgcctcg taatcgcttt cacatccatg tcgtctccct ggctgatgcc
     5161 ggcccggtcg gcgaaatgct cgcatctgcc ggaatccccg tgtcgtcttg cggggcccgc
     5221 acaagccggg acctgaaggc cctcggtcgc ctttggagga ttctcaaagc cacacgaccg
     5281 caagtactgc acgccctgct ctttcacgcc aatatcgcct gccggctgat agggccgctg
     5341 gccgccgtgc cgatcaacag gatcctgtgt gagatccaga cagcggaggt tgagcggcct
     5401 tggcatcttg tgctggataa tctcacctgt cggttatgcc gcttcgaagt cggcaactcg
     5461 ccgtccgtcg tagcccacct gcgtcggaag gcccacctgc cggcctcacg actgttgtgc
     5521 cagtggggcg ccgtagatgt cgcggccatc gacgccgcca agccggctga cagagcaatg
     5581 ttcggcttgt cggctgataa gcccttggtc atctggaccg gccgttttga tcccatcaaa
     5641 ggtttcgagg aaatgctgtc ggcttttgcg cgcctgcgtc aaacactgcc ggcaacctta
     5701 ctgctggtgg gtgacgggcc gtaccgcccg acagttgaac gactgattgc cgagcaaggc
     5761 cttgaccagt gtgtgaagct ggcaggatct cgtcctgacg tgccctcact gttgaagatc
     5821 gcggatgcat ttgtcttctg ttcccggacc gagggattgc ccaattcgct cctcgaggca
     5881 atggcggccg ggttgccggt cgtggctacc gatgttcccg ggtgtcgcga tgtcgtcatc
     5941 gacggaaaaa caggctttct ggcccgggcc ggatctgtcg tcgacatcga gaaaaggctc
     6001 gctcgcgttc tcactgatca tcgcctgtcg gccgacctgg ggactcaggc acgtgactgg
     6061 gttcgtaccc acgtggatgc tcgaaaatgg tccgagcggt ggcaatcgat ttactcctat
     6121 atcgctcaga atgatagtat cactgtacga gccgattgcc tgagcagaca cggctagcgg
     6181 atggattttc cgtcttgccg tttggcgctg acgtcatttg gagttatact tgagttatac
     6241 ttgactgcag gtatcgtcaa atgacgccga tcatttcgga acctgttcgc aggtgatgcg
     6301 caggatgtaa cgctctctaa gacagggggt cgtcatgtcc cagatctcag atgccaagcc
     6361 gctgaccgaa aaaccgatta ccgttgttgt tgtcgatgat catggtattg tccgtgatgg
     6421 cgtgagtggc atgctctcgc gcgaaaaaga cattcgcgtc gtgggcatgg ctgagaatgc
     6481 ggatgaggcc atctccaaag tcgtcgagac caggcccgac gtggtcctca tggacatcga
     6541 tatgccgggc gtgagctgtt tcgatgccgt gcagatcatt cgttcgcgcg tgccaaacac
     6601 caaggtcatt ctggtgaccg cgcacgagca cgacgagtac atcgagcagg cccttcgcgt
     6661 aaaggcgaat ggttacttga tcaagaccca gagcatggcc gccctggccg acgccatccg
     6721 cgatgtagcc aatgggaaaa tccatttctc gcccgaggta atggaccggt tagtggtcaa
     6781 agggggaaag atcgagcttg agaacccgcc aaagacccgc cgcagtatgc tgagcccgcg
     6841 cgaacgcgag ctgttgcgca tcctcgccaa gggcgtcagc ctcaaggaag ccgcgtccat
     6901 tctgggtatc agctacaaga cggccgacaa gcagaaggcc tccctgatgg ccaagctgga
     6961 catccacaac cgcgtcgagc tctcacgatt cgccattcgc gagggcatga tcgaaccgta
     7021 gcagttaccg cacgccgttc attcccggca tgctgtggtc aacaatgcag ccttacggct
     7081 ggacatttgt tcgccttgcg cccatgttgc tggaccgcag tgcgcttggg ttgcggcccg
     7141 caaaaaggcg gtctgccttg ggtgggcgtg cctctcgcgg atacccgctg ccgtattcat
     7201 agtgggcgat agcccaaccg tgatgcctca tcactcggcg tacaaagccg gcgcattggc
     7261 caaggggcct cgctcggcag tcgcgacgcg gatgccggcg ccagactggg tgcgtgacag
     7321 tctgggcggg catgccgacc ggtccaggtt gggcgatcaa ccccgttcag cgggttttcc
     7381 cgctaaccca agttgaacac tttcgaggca agaatggcct tggcgatagc gccaggg
//
LOCUS       DAOTPW010000273         7396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8789_length_7396_cov_5.641738, whole genome shotgun sequence.
ACCESSION   DAOTPW010000273 DAOTPW010000000
VERSION     DAOTPW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8789_length_7396_cov_5.641738"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="P5316_18760"
     CDS             complement(<1..866)
                     /locus_tag="P5316_18760"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRR87057.1"
                     /translation="MFSTIGYVIFVLAPAVLGAILLWRFPKYRRFRLRSILVYLVVMS
                     LLIYGALRFVCYIASATVALREVFIVLWFAIAWRLAWELWAQTVGRLGQKWIRWARLR
                     RHQGLHAPKLIRLIPIGRMLLTAFIFVPAFLSCVVTHRFKLHDDQDPKSIYQMDFEHV
                     RVPTADGLTLDGWFIPQSGASRTIVVCHGAGANKGNFVWFLGSLTRPDYNVVFFDFRA
                     HGSSDGRTTTYGIRERRDVTAVVDWLKRERPAQSQVIVGLGSSQGSMALALAAAEDER
                     IDAVILDSPLTSA"
     gene            1027..2004
                     /locus_tag="P5316_18765"
     CDS             1027..2004
                     /locus_tag="P5316_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR87058.1"
                     /translation="MSNNQPSPNVSRREVIRIAGAAAVGLAGARSALAQSANPLRKRV
                     LRVAHLTDIHIAPEHKAAEGFASCLRHVQSQKDKPDLIFTGGDHIMDSIGANEARTRE
                     QWELYRKVLKAECSLPVEPCIGNHDCWGLNRKDSGATGNEPLYGKKWAMDSLGLDREY
                     RSFDRAGWHFIMLDSTFPQGDGYTARLDDRQFEWLAEDLRRTDPKKPVLILSHIPILA
                     VCVFFDGDNEKSGDWVVPGAWVHIDSRRIKDLFLKHPNVKVCLSGHVHLIDRVEYLGV
                     SYVCNGAVCGGWWEGPYQECDAGYALVDLYDDGSFENRYVPFGWKPVKE"
     gene            2036..3631
                     /locus_tag="P5316_18770"
     CDS             2036..3631
                     /locus_tag="P5316_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR87059.1"
                     /translation="MPITALIPALCVLAAIAPTETVTSQRDNPPVPSSVPLPRITVAG
                     NRFVDPAGRQIILHGVNAGHTSKPDSVRVKPDVCARMRDWGLNVIRLQMEWSQIEPEC
                     GKYDEQYLCLIDEQIAAARAAGLHVYLDMHQDLWGVKSGNGAPAWATLDEGRPHAGNP
                     NLWQESYWTSPMVHAAFDNFYANKSGPDGVGIQDRFAMLWRHVAQRYADDTTVIGYDL
                     FNEPYPGSPMKMLPLLVAAKMTKIRADKGEEVTVAGLLEEYKSEENRRRTMTEVAADE
                     KLLKAVLEALEPLYMTYERDALNPMYARVAKAIREVDRSHIILLEPGGGAIIGVRSAL
                     QPIMDAEGRRDPQQALVPHAYGLPMGDPSTVHMGLIFARISQLAKRLEMPVIVGEWDA
                     DPGNDGDRRPMASFIVSQIEKRLYGDTFWIIKPDLQAQPYFDAIQRPYPMATAGELLS
                     YGYDRDTRVFRCKWNERPSIGQPTRIYLPLAVFPKGFTVELTPSDSKYEQTPCGSGRA
                     GMFIDISPYPNPTNRELTLKPAQ"
     gene            3940..4875
                     /locus_tag="P5316_18775"
     CDS             3940..4875
                     /locus_tag="P5316_18775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRR87060.1"
                     /translation="MNEEAPTQFNEASQDSKETVGTARPVALVVETAPAVEPARRPRV
                     WTVFAAYVSTIVLGTIVTMVHLVITLFGVAPGDWIDELAKRPLSFLWVMSYMLFAALL
                     VAFCAAFLSPVRWVCRLRLTAPSLSCMQVATAAVGAVAIGLTFSMAYALDFLPRSSTL
                     EPLSAVIAGLLGLNLILGIVVIGILPAISEELLFRGYLQTRLTQRWGARISILLTALM
                     FAAYHMDVSQGILALGVGIYLGFVVERGNSIVPAIIGHAVNNTVALLLSVFLPDTLSR
                     ASLWVILLAAAVVLGLCLWHLRSAVPPDRTCMAGA"
     gene            5187..6107
                     /locus_tag="P5316_18780"
     CDS             5187..6107
                     /locus_tag="P5316_18780"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRR87061.1"
                     /translation="MRRRNLTVAILFAGILSVPAWAQNATEPRARRDRPATRRGEGAE
                     GPRNRGMEGPGGRGMMVNQQMERLTKELNLTKEQQEKIQKLMTDHRDQMRDEMQKRIT
                     ENAPKFRELRTQLQEARTAGDREKAGKLESQIRELMGDDKADAARQKLMNEIEAVLTA
                     EQKPKFQKIKDDVFNPRPPLEENPELLLRAVESLKLPKDKADKIKSIVNEWRTKQPRL
                     RPRDRDEKAEQEAKAAAAEVYKKVMAELTPEQQTKVKEWRPAGTMMGWPRGEGQRLRQ
                     GGDEGRGERRAQRRGSSRQGDAPTEDKPKE"
BASE COUNT         1477 a         2366 c         2189 g         1364 t
ORIGIN      
        1 gctgaggtga gcgggctgtc gagaatcacc gcgtcgatcc gctcatcctc ggcggcggcc
       61 agtgccaggg ccatcgaccc ctgggacgat cccagaccga caatgacctg agactgtgcc
      121 ggccgttctc gcttgagcca atcgaccacg gccgtcacgt cgcggcgttc gcggatgccg
      181 taggtcgtgg tccggccgtc gctggaaccg tgagccctga aatcgaaaaa caccacgttg
      241 tagtcgggtc gtgtgagcga cccgaggaac cagacgaagt tacccttgtt ggccccggcc
      301 ccgtggcaga cgacgatggt tcgcgaagcc ccgctctgag ggatgaacca gccgtccaag
      361 gtgaggccgt cggcagtcgg tacgcgaaca tgctcgaagt ccatctggta gatgctcttg
      421 ggatcctgat cgtcgtgcag tttgaaccgg tgagtgacca cgcagctcag aaacgccggc
      481 acgaagatga aggccgtcag cagcatccgg ccgatgggga tcagccggat cagcttcggc
      541 gcgtgcagcc cctgatgcct gcgcagccgt gcccagcgga tccatttctg ccccaaccgg
      601 ccgacggttt gtgcccacag ctcccacgcc agccgccagg cgatggcaaa ccacaggacg
      661 atgaacacct cacgcagggc gaccgtcgcc gaggcgatgt agcacacaaa ccgaagagcc
      721 ccatagatca gcaggctcat caccaccagg tacaccagta tcgatcgcag cctgaaacgc
      781 cggtacttcg ggaaacgcca taggaggatc gcgccgagaa cggccggggc caacacgaag
      841 atgacgtatc cgatcgtgga gaacatggaa ctcccgggcg gcaaggggcc cgtcgttgaa
      901 cacctcggaa ccccaaacaa tatcctgcag ccgccatgat gctaacgttc cccgcgaccc
      961 tgatgcaaac tcgaactgat ccctgaaacc ttgacttcac atgcctcctt ccggttaaat
     1021 ggggcgatga gcaacaacca accatcaccc aacgtttcgc gtcgcgaagt gatccgtatt
     1081 gccggagcgg cggccgttgg gcttgccggt gcccgttccg ccttggcaca gagtgcaaac
     1141 cccttacgca agcgcgtctt gcgggttgcg catctgaccg acattcacat tgcccccgaa
     1201 cacaaggccg ccgaggggtt tgcctcatgt cttcggcacg tgcagtcgca gaaggacaag
     1261 cctgatctga tcttcacggg cggcgaccat atcatggact cgatcggagc gaatgaggcg
     1321 cggaccaggg agcaatggga gctgtaccgc aaggtgctca aggccgagtg cagcctgccg
     1381 gtggagccgt gcatcggaaa ccacgactgt tggggcttga accgcaagga cagcggtgcg
     1441 accggcaacg agcccctgta cggcaagaaa tgggccatgg attctctcgg gctcgatcgc
     1501 gagtaccgca gcttcgatcg cgccgggtgg cacttcatta tgctcgacag cacgttcccc
     1561 cagggcgatg gctacaccgc acggctcgat gaccggcaat tcgagtggct cgccgaagat
     1621 ctacggcgga cggacccgaa gaagcccgtc ctgattctct cgcacattcc gatcctggcc
     1681 gtgtgtgtct tcttcgacgg cgataacgag aagtccggag actgggttgt gccgggggcc
     1741 tgggtccaca ttgacagtcg gcggatcaag gatctcttcc tcaaacaccc gaacgtcaag
     1801 gtctgcctga gtggtcatgt ccacctgatc gatcgggtcg aatacctggg cgtctcatat
     1861 gtctgcaatg gcgccgtctg cggtgggtgg tgggagggcc cctatcagga gtgcgacgcc
     1921 ggctatgcgc tggtcgacct ctacgacgac ggctcattcg agaaccgcta cgtaccgttt
     1981 ggttggaagc ccgtgaagga gtgaccttat ccccgccgcg atcaggctct tgtccatgcc
     2041 tataaccgcg ctgatcccgg ccctttgtgt cctggcggcg atcgcgccga ccgagacagt
     2101 gacttcgcag cgcgacaacc cacccgttcc gtcctctgtc cctctgccgc gaatcactgt
     2161 ggccgggaat cgattcgtcg atcctgccgg ccggcagatc atcttgcacg gcgtcaacgc
     2221 aggccacacc agcaagccgg actcggtccg ggtcaaacct gacgtgtgcg cacgcatgcg
     2281 tgattggggg ctcaacgtca tccgtttaca gatggagtgg tctcagatcg agccggaatg
     2341 tggcaagtat gacgaacaat acctgtgtct catcgacgaa cagatcgctg ccgcccgcgc
     2401 cgccggcctg cacgtttacc tggacatgca ccaggatctc tggggcgtca aatcgggcaa
     2461 cggggccccg gcgtgggcca cgctcgacga aggccggcct cacgctggca acccgaacct
     2521 ctggcaggag tcctactgga caagccccat ggtgcacgcc gcgttcgaca acttctacgc
     2581 gaacaagtcc ggccccgacg gcgtcggcat tcaggaccgc ttcgccatgc tctggcggca
     2641 cgttgcccag cgctacgccg acgataccac cgtcatcggt tacgacttgt tcaacgagcc
     2701 ctatcccggc agtcccatga agatgctgcc gctgcttgtc gccgcgaaga tgaccaagat
     2761 acgggccgac aagggcgaag aggtgaccgt ggccgggctg ctcgaggagt acaagagcga
     2821 ggagaaccgg cgccggacga tgaccgaagt agcggcggat gagaagcttc tcaaggcggt
     2881 tctcgaagcc ctcgaacccc tgtacatgac ctacgagcgc gatgcgctga atccgatgta
     2941 cgctcgcgtg gccaaggcaa tccgcgaagt ggaccgcagc cacatcatcc ttctggagcc
     3001 gggcggaggc gccatcatcg gcgtgcgcag tgctcttcaa cccatcatgg acgccgaagg
     3061 ccggcgcgat cctcagcagg ctctggtgcc gcacgcctac ggcctgccca tgggcgatcc
     3121 ctcgacagta cacatggggc tgatcttcgc ccggatctcg caactggcca aacggctgga
     3181 gatgcccgtc atcgtgggcg aatgggatgc tgaccccggc aacgacggtg accggcggcc
     3241 catggcctcg ttcatcgtct cccagatcga gaaacgtctg tacggcgata cgttctggat
     3301 catcaagccc gatctgcaag cccagccgta ctttgatgcc atccagcggc cctacccgat
     3361 ggccacagcc ggcgagcttc tgagttacgg ctacgaccgc gacacacggg tctttcgctg
     3421 caagtggaac gagcggcctt cgattggcca acccaccagg atctatctcc ccctggccgt
     3481 gttccccaag ggcttcaccg tcgaacttac cccgtctgac tcgaaatacg agcagacccc
     3541 ctgcggttca ggccgcgccg gcatgttcat cgacatctca ccctatccaa accccaccaa
     3601 ccgcgagcta accctgaaac ccgcccaata gcctcctccc ggacggcagg attcgcgctg
     3661 actgccatgt ccaccgcttt gcgtggacat tcttgtgtcc tgatgcaaag caccaccaat
     3721 accccttgcc accgggtatt gacactgcgc aaaaccgccg gtttactgtt cctccgcagt
     3781 ccgggtatct gagagtctgg ctggaacggc aggggcggca agaccagcca agtgacaggt
     3841 gtagcccgac aacgagcagc ctggcgcccc ctgcgatcgt ttgccatgtt tttgcgaccc
     3901 gcgcagccgc cactgcaatc tgacgtcggg gcagcaggaa tgaatgaaga agcgcctacg
     3961 cagttcaacg aggcatccca ggactcaaaa gaaacggtgg gtacagcacg accggttgcg
     4021 ctcgtggtcg agacggctcc ggcggtcgaa cccgcgcgcc gaccgcgcgt gtggaccgtt
     4081 tttgcggcat acgtttcgac gatcgtgctc ggcacaatcg ttacgatggt tcatctcgtg
     4141 atcacgctgt tcggcgtcgc gcctggggac tggatcgatg aactcgcaaa aaggcccttg
     4201 tcctttctgt gggtcatgtc ctacatgttg ttcgccgctc ttctcgtcgc tttctgcgcc
     4261 gcgttcctgt ccccggtgcg gtgggtctgc agactgaggc tcaccgctcc ttccctctcc
     4321 tgcatgcagg tggcaacggc tgcagtcggc gccgttgcca ttggcttgac cttctcgatg
     4381 gcgtatgctc tggacttcct tcctcgctca tccacgcttg agccactatc agcggtcatc
     4441 gccggcctct tggggctgaa tctgatcctg ggcatcgtgg tcataggcat cctgccggct
     4501 atctcggaag agcttctgtt tcgcggctat cttcagacgc gattaaccca gcggtggggc
     4561 gccagaatct ccatccttct caccgctctg atgtttgccg cttatcacat ggatgtttca
     4621 cagggcatcc tcgccctggg cgtgggcatc tacctcggct tcgtagtcga acgggggaac
     4681 agcatcgttc ctgcaatcat cggccacgcg gtcaacaaca ctgtcgctct cctcctcagc
     4741 gtcttcttgc cggacaccct gagtcgggcg tctctgtggg tgatcctgct ggccgccgcc
     4801 gttgtactcg gcctctgcct gtggcatctg cgatcggcgg tgccacctga cagaacttgc
     4861 atggctggtg cctgaccggt gagaaaacct gacaacaact gctctcgccc gactgcaact
     4921 tctgttgcca caatgcgtta gcacaggggc actccacccc agacgtctgg ctgacagcct
     4981 gtcgctgccc gctgagtgct caaaccgcca aagcaaaacc agcatggagc attctcatcc
     5041 aggcaaccat ccggcacttg tcgcaatacg atcgcccggc cggcgttgag cctgctggtt
     5101 gcactgcgag cgcagagggg ttagacgatt agcccttggt gagcagtgga gcctgatgaa
     5161 catttgacgc aagaaaggaa atctccatga gaagaagaaa cctgactgtt gccatactgt
     5221 ttgccggtat tctctcggtt cccgcttggg cccagaacgc aaccgagccc agggcaagac
     5281 gtgaccgccc tgccacgcgc agaggcgaag gagccgaagg gcctcggaac cgtggcatgg
     5341 aggggccggg cggccgtggc atgatggtca accagcagat ggaacgtctg accaaggaac
     5401 tcaaccttac caaggagcag caggagaaaa ttcagaagct gatgaccgac catcgcgacc
     5461 agatgcgtga cgagatgcag aagcgcatca ccgagaacgc ccccaagttc agagagctca
     5521 gaacgcagct acaggaagcc cgaactgccg gcgaccgcga gaaggccgga aagctggaat
     5581 cgcagatccg cgagctgatg ggcgatgaca aggccgacgc tgcccgtcaa aagcttatga
     5641 acgagatcga ggccgtcctc accgccgagc agaaacctaa gttccagaag attaaggatg
     5701 atgtctttaa tccgcgaccc ccgctggagg aaaaccccga gctgctgttg agggctgtcg
     5761 agtcgcttaa actgccgaag gacaaagcgg acaagatcaa gagcatcgtg aacgagtgga
     5821 gaaccaagca gcctaggctt cgcccgaggg acagggacga gaaggccgag caagaggcca
     5881 aggcggctgc ggccgaggtc tataagaagg tgatggccga gctgacgccc gagcaacaga
     5941 cgaaggtcaa agagtggcgt ccggccggta ccatgatggg ttggccgcga ggcgaaggcc
     6001 agagactgcg tcagggtgga gatgagggca gaggcgagcg aagagcccag cgacgcggct
     6061 ccagccgaca gggcgatgcc ccgaccgagg acaagcccaa agagtaggat ccgatatcaa
     6121 tagacctgaa agaggggaca ttctgcttct gtcgaagaat cggaatgtcc gggcgccccg
     6181 gggcggttac ccgcctcggg ctcccacaga tccgtacgtg cgcgcattag cgcatacggt
     6241 tcctcagttt cgtcgtgtgg cagcgcgcgc cctgccacgt tcccgtctgc tatccgctgc
     6301 tgtcacgttc acacggcttt gaagttcgaa gccttcgtca tgtttcctcg caccggttcc
     6361 atgacctgac accccccttc cctccgcagg gtcctccggg gtcgagtatc cctgcttcat
     6421 tggtactacg agggtgctaa gacttcttga cggccatccc gctccgcttc gtttccttcg
     6481 ctcggcagta ccacggccgc atcctgtttt tcgctccgcg tcaccccgga taccctggtg
     6541 accggccagg gatcttcagc tcacgcccgg ctaccccacg cctctccatt ctgtggaccc
     6601 ttgacggtcc tgcattgcgg gaccggctgc aaggcagccc ctggcttcgc cttgcggcgt
     6661 gtcaagggtg gaaccgtcag gacctcccaa gttcctgcga gaccccattg tccgtttgca
     6721 catgctcctg cgactccggc ggaaccgccg acgactcgcc atcacgccgc agcgacacgg
     6781 cccccagtac gggaacgctg aaggcttcca caagggactt tcgaagctca atcgcatggc
     6841 cttcggactc gctgtctacc cttgaccgac ttacgggggt cggtcaaggg tccacagggt
     6901 ctctatgaaa ggtttcaacg actcaactcg ttttcgtctc ctcctctcgc aggttgcttg
     6961 gggcaatccc cttatccgcg ttggcgcagt ttcagaatga cgctggggcg ggccgcatct
     7021 tcgcgcggaa tccattccgc atccgggaat gtggaaagaa ttggctgctg acggccgacc
     7081 tctgcgctgg tcccggattc cgatccggga cccccaggtc gacgattccc atcgtcattc
     7141 acgccggaat gcgggaagga ttgggaccgg cggtcgacct cttggctact cctcgggccg
     7201 ctgaaagccg atcgctgagg gccgaacgct tcaatcgaaa acgccgagac aggataaacg
     7261 cgaagctccg gcccgcagat cgtgggattg acgcagatgg aagacaagtg gggccgacgc
     7321 cctcgtcggc cctttctctc acccggtgcc actggttgcc ggcccgcccg tgcccgatgc
     7381 tcaaacagac ccgcgg
//
LOCUS       DAOTPW010000274         7275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_8962_length_7275_cov_5.201939, whole genome shotgun sequence.
ACCESSION   DAOTPW010000274 DAOTPW010000000
VERSION     DAOTPW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8962_length_7275_cov_5.201939"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="P5316_18785"
     CDS             <1..324
                     /locus_tag="P5316_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR87062.1"
                     /translation="GLHEAGYRLAIETNGTIELPAGLDWITVSPKGPEGRIRQRTANE
                     VKYVLTAGQPLPTSAVRADHYLVSPAFRKGQLDPETLSWCIGLVQDNPPWRLSVQLHK
                     LLGIR"
     gene            485..1498
                     /gene="asnA"
                     /locus_tag="P5316_18790"
     CDS             485..1498
                     /gene="asnA"
                     /locus_tag="P5316_18790"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRR87063.1"
                     /translation="MSYKPLLDLRQTERAIRSIKEFFQTNLAESLNLLRVSAPLFVLA
                     DTGVNDNLNGVERPVSFPIKGMDERRAEIVQSLAKWKRLALADYGFGPGEGLYTDMNA
                     IRPDEVLDEIHSIYVDQWDWERVMRPDERNLNFLYRVVERIYEVIRRTEQHIAAIHPC
                     IKPTLPDKIHFVHSLELEKRWPDLSPRDRENQISKELGAVFIIGIGAGLSNGQPHDGR
                     APDYDDWITPTERGPGLNGDIFVWNPVLNRGFELSSMGIRVNADTLRQQLAIRNAEDR
                     LSLPFHQRLLAGELPQTIGGGIGQSRLCMFFLRKAHIGEVQSSIWPEESREDLRRRGI
                     VLL"
     gene            1665..3281
                     /locus_tag="P5316_18795"
     CDS             1665..3281
                     /locus_tag="P5316_18795"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRR87064.1"
                     /translation="MKKALLMIGGEYHPFETCGWILADFLKSYGTAECTVTSDREAFR
                     KPGGYDVVIVYTQGGKLSKAQEKGLCDWVAGGGAFVGIHCANDSFVGNERYMEMVGTQ
                     FVGHGPVCEFAVNISDQEHEVSRRFSEFRITDEFYLCKRRTKADLHAVMTGKWRFEKQ
                     PVAYVREYGQGRVFYTILGHDERAFGHPAFGKLIHRGVWWATRSNKNETVRCGIVGYG
                     PTFSMGKVHADFIRNTAGLSVTAVCDIDPRRTDAATKELPDVRTFDNHKKMAASGLVD
                     IAINITPHSVHAPLSLDLLRAGIGVVSEKPFCITVEQATRMIRTAREKKVLLTVFHCR
                     RLDADYLAIRKIVDDGLIGEPFHCEAFMGGYQHPGDWWRSHKPISGGAIYDWGAHFVD
                     WILNLMPYRMQSVTGFMSKRVWHDVSNEDHCKAIIRFEGDRSAELEISSIAAVGKPKW
                     RILGTKGGLICDWNKPVQVTRYAGGGREEIEVPYAQDDWSPYYARLADHLLGGEPLMV
                     TPESARRVIAVLELAERSNKSGKAEPVPFE"
     gene            complement(3506..5185)
                     /gene="ilvD"
                     /locus_tag="P5316_18800"
     CDS             complement(3506..5185)
                     /gene="ilvD"
                     /locus_tag="P5316_18800"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRR87065.1"
                     /translation="MPGPLNRFSSRITQPKSQGTSQAMLYAVGLTEDDMKKPQVGIAA
                     VWWEGNPCNMHLNDLALRVKEGVQSAGMVGLRFNTIGVSDGIAMGTDGMSYSLQSREI
                     IADSIETVVAAQWYDGCIAIPGCDKNMPGCVMAMCRLNRPGLVIYGGTIRAGHLGNQA
                     IDIVSASQSYGQYLSGEITAAQRDQIVRCSCPGPGACGGMYTANTMASAIEALGMSLP
                     YSASIPAEDPAKLDECFRAGQMMRNLLELDLKPRDIMTRQAFENAMVVVMALGGSTNS
                     VLHLIAMARTAGVELTIDDFQRVSDRTPFLADLKPSGKYVMEDLHKAGGTPGVMKYLL
                     EKGFLDGGCITVTGKTLAENLADLPGLSPGQDVIHPIERPIKPTGHIRILRGNLAPDG
                     AVAKITGKEGKRFSGPARAFDCEEAMLAAVEKKQIRKGDVVVIRYEGPKGGPGMPEML
                     TPTAAIMSTGLGKDVALITDGRFSGGSHGFIVGHVCPEAQEGGPIALLRDGDVVTIDG
                     ETNTLSVDLPEAELARRRAAWTMPPYKAARGTLYKYIKNVKPASLGCVTDE"
     gene            5474..>7275
                     /gene="ilvD"
                     /locus_tag="P5316_18805"
     CDS             5474..>7275
                     /gene="ilvD"
                     /locus_tag="P5316_18805"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRR87066.1"
                     /translation="MRSDLIKRGVERAPHRSLLKGTGNYTDGDIDKPFIAVCNSYTDI
                     IPGHAHLDRVGYFVKEAVRVAGGVPFIFNTIGIDDGIAMGHAGMKFSLATRELIADCV
                     ESMIEAHRFDAMICIPNCDKIIPGMLMAAARCNIPTIFVSGGPMEAGRTSDGKKVDLI
                     SAFYAVSQHAAGLMDEKTVRDIEDHACPTCGSCSGMFTANSMNCLCEALGMALPGNGT
                     ILATSEDRERLYRRAAERIVEMARADAPKPLDIMTKQAFENAMILDMAMGGSTNTVLH
                     ILAIANEAGVDFTLRDIDALSLKTPNICKIAPSAGDNGRIYHIEDCHAAGGIMTILGE
                     VARGKPGLLHTETITVTGKTLRQNIEDSDIRVRGERVRTDGLSTAKRSELSVYTDNAE
                     KGGAVVVDTIQKGGFDPFDCIRTVEKAYSQQGGLSILYGNLASEGAVVKTAGVDKEML
                     RHSGPAVIFESQEDACEGILKGKVKPGDVVVIRYEGPKGGPGMQEMLAPTSYIKARPE
                     LAKTVALITDGRFSGGTAGACIGHVSPEAAAGGTIGLLRNGDVIEIDIPGHRLSVKLS
                     DAEIAERRKTWTPPRPRYTKGCLAKYSALATSAST"
BASE COUNT         1395 a         2119 c         2395 g         1366 t
ORIGIN      
        1 ggactgcacg aggccggcta cagactggcc atcgaaacca acggcaccat cgagttacca
       61 gccgggctcg actggatcac cgtgagcccg aaggggccgg agggccgtat ccgacagcgg
      121 acggcaaacg aggtgaaata cgtgctgacg gccgggcagc cgctcccgac ttccgctgtt
      181 cgggcggacc attacctggt ctctccggcc tttcgcaaag gccaactgga tcccgaaacg
      241 ctttcctggt gcattgggct tgttcaagac aatccgccgt ggcggctgtc ggtgcaactg
      301 cacaagctgc tgggcattcg gtaggcgaga tcccgggcct gcctgacgaa cccgcttgcg
      361 ggcaacctgt ccgcagtggt ttgtctcggc cggatcgccc ggctaccatg tggcccccgt
      421 ttgttcgaac gggaggctgg aaggagagcc ggattcgacc ggcggtcatt accgtggaga
      481 tggcatgagt tacaagccgt tgctcgactt gaggcagact gaaagggcga ttcgctcgat
      541 caaggagttt tttcagacga atctggccga atcgctcaac cttttgcggg tgagcgctcc
      601 gctgttcgtg ctggcggaca cgggggtcaa cgacaacctg aacggcgttg agcggccggt
      661 gtcctttccg atcaagggca tggatgaacg gcgggcggag atcgtccagt cgctcgcgaa
      721 gtggaagcgg ttggccctag ccgactacgg tttcggtccg ggcgaggggt tgtatacgga
      781 catgaacgcc atccggcccg acgaggtgct cgacgagatt cactccattt atgtggatca
      841 gtgggactgg gagcgggtca tgcggccgga cgagcggaac ctgaactttc tgtaccgggt
      901 cgtcgaacgc atctatgagg tgatccggcg aactgagcag cacattgccg ccattcaccc
      961 gtgcatcaag ccgacgctgc cggacaagat ccatttcgtg cactcgctgg aactggagaa
     1021 gcgctggccg gacctgtcgc cccgggatcg cgagaatcag attagcaagg agcttggggc
     1081 ggtattcatc atcggcatcg gagccgggtt gtcgaacggt cagccccacg acgggcgggc
     1141 ccccgactat gacgattgga tcactcccac cgagcgcggg ccggggctca acggtgacat
     1201 cttcgtgtgg aacccggtgc tcaaccgggg tttcgagctt tcttcgatgg gcatccgcgt
     1261 gaacgccgat acgctcaggc agcagcttgc gatccgaaac gccgaggacc gtctcagcct
     1321 gccgttccat cagcggcttc tggccggcga actgccgcag accatcggcg gcggcatcgg
     1381 ccaatcgcgc ctctgcatgt tctttctgcg taaggctcac atcggcgagg tacagagcag
     1441 catctggccc gaagaaagcc gggaagatct ccgacggcgc ggcattgtcc ttttgtagaa
     1501 ggcatgcggt gcctgtctgc attgacgccg gcgcccgcat accggaacat gtttctgcgg
     1561 ttgcataata atgaagagaa gacgagggcg ccacacgtcg ggcgggctta cctccggcgg
     1621 gcacaagcac ctgctaaaaa gtgttgttga ggagttttgc ttctatgaag aaagcgttgt
     1681 tgatgatcgg gggggagtat cacccgttcg aaacctgcgg gtggatcctg gcggatttcc
     1741 tcaagagcta cggcacagcc gagtgtacgg tcacgtcgga ccgtgaagcg ttcaggaaac
     1801 cgggcggcta tgacgtggtg atcgtttaca cccagggcgg caagctgagc aaggcgcagg
     1861 agaagggtct gtgcgactgg gtggccggcg gcggggcatt cgtcggcatc cactgcgcca
     1921 acgattcctt cgtcggcaac gagcggtaca tggagatggt cggcacacag tttgtggggc
     1981 atgggccggt ttgcgagttc gccgtcaaca tctccgacca ggagcacgaa gtgtcgcgtc
     2041 gtttcagcga gttccgcatc acggacgagt tttatctgtg caagcgacgg acgaaggccg
     2101 acctgcacgc ggtgatgacc ggcaagtggc ggttcgagaa gcagccggtc gcctacgttc
     2161 gcgaatacgg ccagggacgc gtgttctaca ccattctggg ccacgacgag cgggcctttg
     2221 gccatccagc cttcgggaaa ctgatccacc ggggcgtctg gtgggctacg cgatcgaaca
     2281 agaatgaaac ggtgcgatgc ggcattgtcg gatatggtcc gaccttcagc atgggcaaag
     2341 tgcacgccga cttcattcgc aataccgcag gcctgagcgt gacggccgtt tgcgatatcg
     2401 atccgagacg gaccgacgcc gcgaccaaag aactgccgga cgttcgcacc ttcgacaatc
     2461 acaagaagat ggccgcttcc ggcctggtgg atatcgcgat caacattacc ccgcatagcg
     2521 tgcatgcccc gttgtcgctt gacctgttga gggcgggcat cggtgtggtc tcagagaagc
     2581 ctttctgcat caccgtcgag caggcgacga ggatgatcag gaccgcgcgc gaaaaaaagg
     2641 tcctcctcac tgtttttcat tgccggcggc tggatgccga ttacctggcg atcaggaaga
     2701 tcgtggacga cgggctcatc ggcgagcctt ttcactgcga ggcgttcatg ggcggctatc
     2761 agcatcccgg cgactggtgg cgatcgcaca agccgatcag cggcggtgcg atttatgact
     2821 ggggagcgca ctttgtcgac tggattctca acttgatgcc ttacaggatg caatcggtta
     2881 ccggctttat gagcaagcgc gtctggcacg acgtcagcaa cgaggaccac tgcaaggcga
     2941 tcatccgttt cgagggcgat cggtcggccg aactggagat ttcgagcatt gcagccgtcg
     3001 gcaagccgaa gtggcggatc ttaggcacga agggcgggct tatttgcgac tggaacaagc
     3061 cggtacaggt gacccgttac gcgggcggcg gcagggagga gatcgaggtg ccatacgcgc
     3121 aggatgactg gtcgccgtac tacgcgagat tggccgatca cctgttggga ggcgaaccgc
     3181 tgatggtcac gccggaaagt gcccggcggg tgatcgcggt gttggaactg gcggaaaggt
     3241 caaacaagtc gggcaaggcc gagccggtgc cgtttgaatg agtggccggt ccgagcggca
     3301 ggtctctgca gcctcatcgc tggcctcaga atccattctg gggcctttcg cgtggaggat
     3361 ttccatcctc atttgcggcg aatgcggaac ggattccgcc aaaggaggcg gcccggaatg
     3421 gattgcgggc cgagcgggag gtctccggcg gcttcgacca tgccctaccc ttctcacacg
     3481 tgctctgagt ggccccgttg gtccgtcatt catccgtcac gcagccgagc gaagccggct
     3541 tgacgttctt gatgtacttg tacagcgtgc cgcgggcggc cttgtagggt ggcatcgtcc
     3601 aggcggcgcg gcgacgggcc agctccgcct ccggcagatc aacggacagg gtattcgtct
     3661 cgccgtcaat ggtgacgacg tcgccatcgc gaagcagggc gatgggcccg ccttcctgag
     3721 cctccgggca gacgtggccg acgatgaaac cgtgtgagcc gcccgagaaa cgtccgtcgg
     3781 tgatcaacgc gacgtccttg ccaaggccgg tggacatgat tgccgcagtt ggcgtaagca
     3841 tttcgggcat accagggccg cctttgggac cttcgtaacg gatgaccacc acgtcgccct
     3901 tgcggatttg ctttttctcc acggccgcga gcatagcctc ttcgcaatcg aacgcgcggg
     3961 ccggcccgct gaaacgcttt ccttccttgc cggtgatctt ggcgactgcg ccgtctgggg
     4021 cgaggttgcc gcgaagaatg cggatgtgac cggtaggctt gatcggtcgc tcgatggggt
     4081 ggatcacgtc ctggcccgga ctcaggcccg gcaggtcggc caggttctcg gccagcgttt
     4141 tgccggtcac ggtgatgcaa ccgccatcca gaaagccttt ttccagcagg tatttcatga
     4201 cccccggcgt tccgccggcc ttgtgcagat cttccatgac gtacttgccg ctgggtttca
     4261 ggtcggccag gaacggcgtg cggtcggaga cccgctggaa atcatcgatg gtcaactcga
     4321 cgccggccgt gcgggccatg gcaatcaggt gcaggactga gtttgtggag ccccccagag
     4381 ccatcaccac taccatggcg ttctcgaacg cctggcgggt catgatgtcg cggggcttga
     4441 ggtcgagctc caggaggttt cgcatcatct ggccggcgcg gaagcactca tcgagtttcg
     4501 ctgggtcctc tgccgggatc gacgcgctgt acggcaagga catgccgaga gcttcgatcg
     4561 cgctggccat ggtgtttgcg gtgtacattc cgccacaggc acccgggccg gggcaactgc
     4621 aacggacgat ctggtcgcgc tgggcggcag tgatctctcc agacaggtac tggccataag
     4681 actgagacgc cgagacgata tcgatggctt gattgccgag gtggccggcg cggatggtcc
     4741 cgccgtaaat caccaagccg gggcggttga gccggcacat ggccatgacg cagccgggca
     4801 tgttcttgtc gcagccggga atggcgatgc agccatcgta ccactgggcg gccacgaccg
     4861 tctcgatcga gtcggcaatg atctcgcggg attgaagcga gtagctcatg ccgtcggtgc
     4921 ccatggcgat gccgtcgctg actccgatgg tgttgaaccg caacccgacc atacccgcgg
     4981 actgcacgcc ctccttgact cgcaaggcca ggtcgttcag gtgcatgttg caggggttgc
     5041 cctcccacca cacggcggcg atgcccacct gcggtttttt catgtcgtcc tcggtcaggc
     5101 cgacggcgta aagcatcgcc tgagacgtcc cctgcgactt gggctgggtg atgcgggagc
     5161 tgaacctgtt tagtggtccg ggcatgatag cactcctatt atcagcaatt gcgagacgat
     5221 tatagccatg ttgtcaggtg atgccatgct tgcggccgga gcacggcggg ccgccggcgg
     5281 cttgtccacg gctcggcgcg ggcatgtccg gccgatttga catcgcaaat gcaggatggg
     5341 agaccatagc ggaggcagaa gttgcagtct gtgatgatgg ggtataatgt cgcctccacg
     5401 ccggcgggga cgcagccttg atgacgctgg cctgtagaag ctttccaagg ttctaacaag
     5461 aggttgaatt gctatgcgaa gtgatctgat caagcgaggc gttgagcggg cgccgcatcg
     5521 gagcctgctg aagggcacgg gcaattatac ggatggcgac atcgacaagc cgttcatcgc
     5581 ggtgtgcaat tcgtacaccg acatcatccc cggccatgcc cacctggacc gtgtcgggta
     5641 ttttgtcaag gaggccgttc gggtcgccgg cggcgtgcct ttcatcttca ataccattgg
     5701 gatcgacgac ggcatcgcca tgggccacgc cggcatgaag ttttcgctgg ccacgcgcga
     5761 actgatcgcc gattgcgtcg agtcgatgat cgaggcacac cgcttcgatg cgatgatctg
     5821 cattcccaac tgcgacaaga tcatcccggg gatgctcatg gccgcggccc ggtgcaatat
     5881 tccgaccatc tttgtcagcg gcgggccgat ggaggcgggc cgtaccagcg acggcaagaa
     5941 agtcgatttg atcagcgctt tctacgcggt gagccagcac gcggccggcc tgatggatga
     6001 gaagaccgtt cgcgatattg aggaccacgc ctgtccgacg tgcgggagct gctcgggcat
     6061 gttcaccgcc aacagcatga actgtttgtg cgaggccctg ggcatggctc tgccgggcaa
     6121 cggaaccatc ctggccacca gtgaggatcg cgagagactg tatcgccggg cggccgagcg
     6181 aatcgtcgag atggccaggg ccgacgcgcc caagccgctg gacatcatga ccaagcaggc
     6241 cttcgagaat gcgatgattc tggacatggc catgggcggc tcgaccaaca ccgtgctgca
     6301 catcctggcg attgccaatg aggccggcgt cgatttcacg ctcagggaca tcgatgccct
     6361 gtcgctcaag acgccgaaca tctgcaagat tgcgccgtct gcgggcgaca acggtcgtat
     6421 ttaccacatt gaggattgtc acgccgccgg cgggatcatg accattctgg gcgaggtggc
     6481 tcgcggcaag cccggcttgc ttcataccga gacgatcacc gtcaccggca agacgctgag
     6541 gcagaacatc gaggacagcg acatccgcgt tcgcggcgag agggtccgca cagacgggct
     6601 gagcaccgcc aagcgaagcg aactcagcgt ctacaccgac aacgccgaaa agggcggagc
     6661 ggtggtggtg gacaccattc agaagggcgg ttttgatccc ttcgactgca ttcggacggt
     6721 tgagaaggcc tattcgcagc agggcgggct gagcatcctc tacggcaacc ttgcttccga
     6781 gggtgcggtg gtgaagaccg caggcgtgga caaggagatg ctcaggcaca gcggcccggc
     6841 ggtgatcttc gaaagccagg aggacgcctg cgagggcatc ttgaagggca aggtcaagcc
     6901 cggtgacgtg gtggttattc gctacgaagg cccgaagggc ggcccgggca tgcaggagat
     6961 gctcgctccg accagctaca tcaaggcacg gccggaactg gcgaagaccg tggccctgat
     7021 taccgacggg cgcttcagcg gcgggaccgc aggcgcctgc atcggccacg tcagtccaga
     7081 agctgcggcc ggaggcacca tcggcttgct ccgcaacggc gacgtcatcg aaatcgatat
     7141 tcccggtcac aggctgagtg tcaagctgtc cgacgccgaa atcgccgagc gtcgcaagac
     7201 ctggacgccg cccagaccgc gctacaccaa gggctgcctg gccaagtaca gcgccctggc
     7261 caccagcgcg agcac
//
LOCUS       DAOTPW010000275         7214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9041_length_7214_cov_4.729990, whole genome shotgun sequence.
ACCESSION   DAOTPW010000275 DAOTPW010000000
VERSION     DAOTPW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9041_length_7214_cov_4.729990"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..1448)
                     /gene="purM"
                     /locus_tag="P5316_18810"
     CDS             complement(375..1448)
                     /gene="purM"
                     /locus_tag="P5316_18810"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRR87067.1"
                     /translation="MGKRKKTITYKDSGVDIDANDVMVELIKPIVRSTYGPRVFGKHG
                     GFAGLFRLDYCEALFKRNYKEPVLVACTDGVGSKILLARDSGHYDTVGIDLVAMNVND
                     MLTLGAEPLFFLDYVAVNKLDPKRVAQIVAGIAAGCRESDCSLIGGETAELPDIYKPD
                     DFDLAGFAVGVVERKKIIDGSEIEAGDLIIGLPSTGLHSNGYALARRLVFKEARLKYD
                     SVVPMIGGPIGDELLRPTRIYVMPVLDLLHNYRVKRVVRGMAHITGGGLPGNVDRVIP
                     DGLKAVINTKSWTPPPVFRYLQSLGVRRDEMFRVFNMGIGYILIVRPAFAHSIVGRFR
                     RRHQLDALIIGRIEKGPTTVELV"
     gene            1992..2318
                     /locus_tag="P5316_18815"
                     /pseudo
     CDS             1992..2318
                     /locus_tag="P5316_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
     gene            2661..4073
                     /locus_tag="P5316_18820"
     CDS             2661..4073
                     /locus_tag="P5316_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87068.1"
                     /translation="MVVRVGVDGSVEQGYDTTGDGKADCSERVNATGVVEAIRFDDNK
                     DGCLDDRDAWVVLADVPEQERRHLIMILDSVPFAMVESLWAEGRFRLFSQPSRVISPF
                     PVMTDLCLSDFFGVSPCPGVESEYFDGEQLRNGYVAYASKENVPWMDLVDWHLEPATH
                     VSVYLGAKKWYLHELRQVQEHFRGSGGPRVIAYIVGTSALGARVGRDGHQAGLIQLDR
                     FCRQLVFESRGRVRISLLSDHGHHYSSSKRIPLSRLLAEFGYRPGRGLERSGDVVVPE
                     FGMVSCAVIHTREAAEVARDVLGIEGVELTAYRDASDNLVVLSRTGRARITHSAGRFA
                     YRADFGDPLDLLPIYASLRAEGKVDADGFAGDGVLKEATATHSYPDVVHRLWRAFHGL
                     VQHTPDVLVSTQDGYHCGSAFMSSIVHLTAAHGNLRRPSSTGFSMSMNGRLPEMVRME
                     DLRGELVRLGVPLDRLESRH"
     gene            complement(4176..5213)
                     /gene="trpD"
                     /locus_tag="P5316_18825"
     CDS             complement(4176..5213)
                     /gene="trpD"
                     /locus_tag="P5316_18825"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRR87069.1"
                     /translation="MTISIHEIIAHLSAGRHLSREHMREIFELIMTGRVEPGPMGDFL
                     KALASKGETVDEIAGAADVMNEKVTRVRCDADCIDTCGTGGDRISTLNVSSTAAIIAA
                     AAGAVVAKHGNRTNTRISGSAEVFAALGVNLDAPVPVLERCLRDCGIAFLYAPNLHPA
                     MRYAGPVRKQLGIRTIFNYLGPFTNPAGARRQVMGVARPEMTETLAGVLAARGATLAW
                     VVTGYTGVADHPYLCDITITGNTKVSEVRNGQVRTFTINPSDVGFDMAPLDSLLIDSV
                     QASADAVLAILSGRDQGPRRQHALLNAGAALVVAGLAPDLPAAIAKAAQTLDSGAAMA
                     KLEQLVRTSKA"
     gene            complement(5258..6430)
                     /gene="galK"
                     /locus_tag="P5316_18830"
     CDS             complement(5258..6430)
                     /gene="galK"
                     /locus_tag="P5316_18830"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRR87070.1"
                     /translation="MSMVNTGELKDLHARLYPGSAPRVAVRAPGRVNLIGEHTDYNDG
                     CVLPIAMSQALYVTASPSPDGRIAVHSNAFNETVEFPADNPGSPCDPAWANYVRGVAA
                     MLIRQGIPLKPCRLAIHSDVPLGAGVSSSAALEVGSALALLAMAEQRIQAIPLALLAR
                     QAEHEYAHSPCGIMDQFICVLGKARHALMLDCRSQTYEQIPLPFENTTLVIMNTQVKH
                     AIGSSQYPVRQKQCREGLTILQRSDPRLIALRDAHMGMLLQHRSRMDEVVFRRCRHVI
                     TEIARVPLAAEAMKAGDAATFGRLMFESHQSLKDDYEVSCTELDTLVDIARQVPGVYG
                     ARMTGGGFGGCAIALIDSGSEHALRAAVKNKYDTRFEQPAIVYTTTASEGATIIPL"
     gene            6576..>7214
                     /locus_tag="P5316_18835"
     CDS             6576..>7214
                     /locus_tag="P5316_18835"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRR87071.1"
                     /translation="MFRPVLIDTDMGVDDAVAIALALSSSEIQVAGLVSVAGNVSIEQ
                     ATANIGRLLGGLVPESLPQVARGLGHRVSQLRDATHVFGEDGLGGIELAVPPDFAPGE
                     FLELYERLAEQHGHSLTVLALGPLTNLAAVLQEKPEVLAKVGQIVVMGGAIWCPGNVT
                     RWAEFNFYRDPAAAAAVLSAGLPLTVVPLDVTRQVAMDESHTAHLSRGGTRAG"
BASE COUNT         1293 a         1914 c         2581 g         1426 t
ORIGIN      
        1 tgggaggatg gccggattga gccttgcgaa gggcagattc ctgccctaat ctccgccgag
       61 aacgggtgtt gcggagttat ccacggtggg ttcgttcagc gcggtcgggg agagggagat
      121 cagggatttc agatttgagg cttcggaaaa acaagcgagg gggagagatt ctccattttg
      181 acatttgggt ttgtttcgct ggacgagccg cggggttttc gtgctgagcg accaatcttt
      241 ggttgtcaaa gagcgcgcag tctgtccggg tgctcggtgc gggacgagaa cacacgtggt
      301 gatccggagg tggtgcgggt tgcggagcag gtgggccgag caattgctga tggcccgatc
      361 accatgaccg atagctaaac gagttccacg gtggtcgggc ctttttcgat ccggccgatg
      421 atgagggcgt caagttgatg gcggcggcgg aagcgaccga cgatggagtg ggcgaaggcc
      481 ggtcggacga tgaggatgta gcctattccc atgttgaaga cccggaacat ttcgtcgcgt
      541 ctcacgccga gggattgcag gtagcggaaa acaggcgggg gcgtccagct cttagtgttg
      601 atcacggcct tgaggccgtc ggggatgacg cggtcgacgt tgccgggcag gccgccgcca
      661 gtgatgtggg ccatgccgcg gacgacgcgc ttgaccctat agttgtgcag aaggtcgagc
      721 acgggcataa cgtagatccg cgttggacgg aggagctcgt cgccgatcgg cccgccgatc
      781 atcggcacta ctgaatcata cttaagccgg gcttctttga agaccagccg ccgggcgaga
      841 gcgtagccgt tggaatggag gccggtcgag ggcaggccga tgatcaagtc gccggcctcg
      901 atttcggaac cgtcgatgat cttctttcgt tcgactacgc cgacggcaaa tccggccaga
      961 tcgaagtcgt cgggtttgta gatgtcgggc agctcagccg tctcgccgcc gatgagggag
     1021 caatctgatt cgcggcatcc ggcggcgatt ccggccacga tctgggcgac acgtttggga
     1081 tcaagcttgt taacagccac gtaatcgaga aagaagaggg gctcggcccc gagggtcagc
     1141 atgtcgttga cgttcatggc caccaggtca atgccgacgg tgtcgtagtg accggagtcg
     1201 cgggccagca gaattttgct gccgacgccg tcggtgcagg cgacgaggac gggctccttg
     1261 tagttgcgtt tgaacagagc ttcgcagtaa tcgaggcgga acaggccggc gaaaccgccg
     1321 tgcttgccga acacgcgggg gccgtaggtg ctgcgaacga tgggcttgat cagctcgacc
     1381 atgacgtcgt tcgcgtcgat gtccacgccg ctgtctttgt aagtgatcgt ctttttgcgt
     1441 ttgcccatcc cgacaatcta acgggcgggc agggggcaga caactccgac aagggcaagt
     1501 gaaggatgaa gacggcagga tgaaggagga atcaaggcca ggtcctgtga gttcagtttc
     1561 gcgcccgctg ggaggtgggg cggctgattg ccggccgctg aacggcgaca gctttctgcc
     1621 accgttatct tgagaagctc attcagcgag ccgaagcacg tgatacgaaa ggccacgtat
     1681 cgagcctgaa gatgcttgac gcgattgccg gattatcggc cctagctgct gggatagtcg
     1741 cgccggtcgc gctgcggcgc gtctctggtc tgtctcaagt caggcctgag cccaagccgc
     1801 agccgaggga cggagaccgg ctggagctgt cagcggggca catccatgag gaaggccgga
     1861 cttgcccact ttgcggagtg gagatcagcg gccaagacgg atgggcgtca ccgagctggg
     1921 tcgcaaccga gcgtgccttg ggaggggaga cacaaggcgg gttggttgat ttgggcaagg
     1981 tgctgaccgc cgaggaggaa gagaagctgg agaagctcaa agagcgcgac cgggaggtcc
     2041 gcgcacacga gcaggctcac aaggcggccg gtgggcaatg cgcgggcagc gccagctaca
     2101 gctatcagac cggcccggat ggccgtccgt atgccgtcgg cggtgaggtt tccgtagacc
     2161 tctcgccggt gccgggtgat ccgcaggcta cgatcgccaa gatgcagcag attcggcggg
     2221 cagctttggc cccggcccag ccgtcatctc aggaccggct ggtggcagca caagccggtc
     2281 gtattgaagc caaggcacgc gccgagctgg ccgagaagaa gaccgagggg gcatccagtg
     2341 aggggttgtc cggcgacggt gttgctggtc aggagagcag caccgaacgc cgctataccg
     2401 acgaagcagc cggcgaaggc agcccggcag ggtttctact ggatattgcc atctgactcg
     2461 ccgccattta gcattccatc cacatcacaa agccgcgtat tagcagagat cgccaggtga
     2521 ttgttgttga gtcggaccca gttatgttca gatggttttg tcttacggtg cggagggtgg
     2581 ttgttctcaa ttgcgtcgtg tcggcgctgg tcggttgcgc gcttccgggc ccgggcccgg
     2641 agctgttccc ggtgacgccc atggtcgttc gagtcggggt cgacgggagc gtggagcagg
     2701 gttatgacac gacgggtgac ggcaaagccg attgcagcga acgcgtgaat gcgacggggg
     2761 tcgtcgaggc tatccgattt gacgacaaca aggacggctg ccttgacgat cgcgacgcgt
     2821 gggtcgtgct ggctgacgtt cctgagcagg aacggcgtca tctgatcatg atcctggaca
     2881 gcgttccgtt tgcgatggtg gagagtctgt gggccgaagg tcgttttcgc ctgttttcgc
     2941 agccgagtcg ggtcatcagc ccgtttccgg tgatgaccga tttgtgtctg tcagactttt
     3001 tcggcgtttc gccctgcccg ggtgtggagt cggagtactt cgacggggag cagctcagga
     3061 acgggtacgt cgcctatgcg agcaaggaga atgttccctg gatggatctt gtggactggc
     3121 atctggagcc ggcgactcac gtatcggtct atcttggggc gaagaaatgg tacctgcacg
     3181 agcttcgaca ggtgcaggag cactttcggg ggagcggcgg accgcgggtg atcgcctaca
     3241 tcgttgggac gtcggctctt ggagccagag tcgggcgtga cgggcatcaa gcgggcttga
     3301 tccagttgga ccgtttttgc cggcaactgg tatttgagtc ccgcggtcgc gtacggatca
     3361 gccttctttc ggatcacggt caccattatt cgagcagcaa gcgaattcct ctgagcaggc
     3421 tgctggcgga gttcggctat cgtcccggga gggggcttga acgttccggc gacgtagtgg
     3481 tgcccgagtt tgggatggtg agttgcgccg tgattcacac acgagaggcg gcggaagttg
     3541 cacgagacgt tttggggatc gagggcgtgg aactgaccgc ctatcgcgat gcctcggata
     3601 atcttgtcgt tctgagccgg acggggcgtg cgcggatcac gcattcggcg ggtcgattcg
     3661 cgtaccgggc cgacttcggc gacccgctcg atctcctgcc gatctacgcg tcgttgcggg
     3721 ccgagggcaa ggtggacgct gatggtttcg ccggtgacgg agtgctgaag gaggcaacgg
     3781 cgactcacag ctaccccgac gtcgtgcatc ggctatggcg ggctttccac gggttggttc
     3841 agcatactcc tgacgtgctg gtgtccacgc aggacggcta ccattgcggc agcgcgttca
     3901 tgtcgagcat cgttcatctt acggccgcgc acggcaacct gcgcaggccg agctcaacgg
     3961 gtttcagcat gtcgatgaat ggtcgcttac ctgagatggt ccggatggag gatttgcgcg
     4021 gggagcttgt tcggctgggt gtgccgcttg acaggctcga aagccgtcac tgagaacgct
     4081 cgcgaacagg gtgggccgga gccaagtccg cgtgaggtcg cggtcgtcgc ggcgaccaaa
     4141 accctgccct gccggtggga aggtttcagc cgggttcaag ccttggacgt tcttaccaac
     4201 tgctccaact tggccatggc ggcgccggag tcaagggtct gggcagcttt ggcgatggcg
     4261 gccggcaggt cgggggccag accggcgaca accagggcgg cgccggcgtt gagtaaggcg
     4321 tgttggcgac gagggccttg gtcccgaccc gaaaggatgg caaggacggc gtcggcactg
     4381 gcctgtacgg aatcgatcaa cagggaatcg agtggggcca tgtcgaagcc gacatcggac
     4441 ggattgatcg tgaaggttct gacttgtccg ttgcggacct cgctgacctt ggtgttgccg
     4501 gtgatggtga tatcgcagag atatggatgg tcggcgacgc cggtatatcc ggtgacgacc
     4561 caggcgaggg ttgccccgcg ggcggcgagc acgccggcca gtgtttcggt catttcggga
     4621 cgggcgactc ccatgacttg ccgtctggcc cccgcgggat tggtgaatgg tccgagatag
     4681 ttaaagattg tgcggatacc cagttgtttg cggacgggac cggcatatct cattgccggg
     4741 tgcaagttgg gtgcatacag gaaggcgatg ccgcaatccc gcaggcagcg ttcgaggacg
     4801 gggacgggcg cgtcgagatt cacacctaaa gcggcgaaca cctcggccga tccgctgatg
     4861 cgggtgtttg ttcgattgcc gtgcttggcg acgacagcgc cggcggcagc ggcgatgatg
     4921 gccgcagtgg atgagacgtt gagcgtactg attctgtcgc cgccggtgcc gcaggtatca
     4981 atgcaatcgg catcacatcg gacgcgcgtg accttttcgt tcatcacgtc ggctgcgccg
     5041 gcgatttcat cgacggtctc acccttggag gcaagggctt tgaggaagtc gcccattggt
     5101 ccgggctcga cccggccggt catgatgagt tcgaagattt cgcgcatgtg ttcccgcgac
     5161 aggtggcgac cggcactgag gtgagcgatg atttcatgga tcgagatggt cattgttttc
     5221 tccgtcacag tcggagcctg cggggaagca ggcgttatca aagcgggatg atggttgcgc
     5281 cctccgaggc cgtggtggtg tagacaatcg cgggctgctc gaagcgtgtg tcgtacttgt
     5341 tcttgacggc ggctcgcaaa gcgtgttcgg agccgctgtc gatcaaggcg attgcgcagc
     5401 caccgaaacc gccgccggtc atccgggcgc cataaacccc gggtacctgc cgggcgatat
     5461 cgaccaaggt atcgagttcg gtgcaagaca cctcataatc atccttcagg gactggtggg
     5521 attcgaacat gagccggccg aaggtcgcgg cgtcgccggc cttcatggct tcggcggcca
     5581 gcggcacgcg agcgatttct gtgatgacat ggcggcatcg ccggaagacc acttcgtcca
     5641 tgcgggaccg gtgctgaaga agcatgccca tgtgggcatc gcgcaaggcg atcagcctgg
     5701 ggtcggatcg ctggaggatg gtcagcccct cgcggcactg tttctgccgg acggggtatt
     5761 ggctggatcc gatggcgtgc ttcacctgcg tattcataat aaccagcgta gtgttctcga
     5821 aaggcagagg aatctgctcg taggtttgtg agcggcaatc gagcatcagt gcatggcggg
     5881 ctttcccgag gacgcagata aactggtcca tgatgccgca tggcgaatgg gcgtactcat
     5941 gctcggcctg gcgagccagc agggccaggg ggatggcctg gatgcgttgc tcagccattg
     6001 ccaacagggc caaggcggag ccgacctcaa gggcggccga gctgctcacg ccggcgccca
     6061 aggggacatc gctgtggatg gcgaggcggc agggtttgag ggggattccc tgacggatga
     6121 gcatggctgc gacaccgcgg acgtagtttg cccacgcagg atcgcacggc gagccgggat
     6181 tgtcggcggg aaactcgacc gtctcattga acgcgttgga atgcacggcg atgcggccgt
     6241 cgggcgaggg gcttgcggtg acgtacaagg cctgagacat ggcgatcggc aggacgcagc
     6301 cgtcgttgta atcggtgtgt tcgccgatga ggtttacccg acctggcgcc cgcacggcca
     6361 cgcgtggggc cgaacccggg taaaggcggg cgtgcaggtc cttcagttcg ccggtattga
     6421 ccatgctcat gatctcctca acaataggcg gtgcgactgt agggcgtcag ttcaggcctg
     6481 tcaagaaacg cccaggcgtg tacccgaaga tggagcggcg aggtgcccgc cctatactgg
     6541 gggcggtgga ggcccaggga attggtggac ctgtcatgtt ccggccggtt ctgatagaca
     6601 cggacatggg tgtggatgat gccgtggcga ttgcgctggc actcagttcc agtgaaatcc
     6661 aagtagcggg tctggtcagc gtggcgggca acgtgtcgat cgaacaggcg acggccaaca
     6721 tcgggcgtct gctcggcggt ctggtgccgg agtcgttgcc tcaggtggcc cgtggtttgg
     6781 ggcaccgagt ttcgcagttg agagatgcga cgcacgtgtt cggcgaggat ggcctgggcg
     6841 gaatcgagct ggcagtgccg cccgattttg cgccgggcga attcctggag ctttacgagc
     6901 ggcttgccga gcagcatggc cactcgctga cggtgttagc ccttggtccg ctgaccaatc
     6961 ttgcggcggt gttgcaggag aagccggagg ttttggccaa ggtgggccag atcgtcgtca
     7021 tgggcggggc tatctggtgt ccgggcaatg ttacccgctg ggctgagttc aatttttacc
     7081 gcgacccggc ggcggccgcg gcggtgttat cggcggggtt gccgctgacg gtggtgccgc
     7141 tggatgtgac gcggcaggtg gcgatggatg aatctcacac cgctcacctg tctcgggggg
     7201 ggacgcgggc gggg
//
LOCUS       DAOTPW010000276         7191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9080_length_7191_cov_5.253083, whole genome shotgun sequence.
ACCESSION   DAOTPW010000276 DAOTPW010000000
VERSION     DAOTPW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9080_length_7191_cov_5.253083"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="P5316_18840"
     CDS             <1..1230
                     /locus_tag="P5316_18840"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRR87072.1"
                     /translation="ASFDNRSCRIHTVKSGEVIGEYVPETPPQPGMDVYGKPIPGAPS
                     LLRFELGANVKLADDGKTVYATSNGMVRVTRQGVSVVEQVKVDGDVDLKSGNVDAPSD
                     VLITGNVVETFLVRSPKSITIKGMVEAANVEAGSHVIIAGGVAGKSKALIRAGGELQV
                     KFLEEAAIEATGDVIVTKEVMNCRIRTMGRLLVPRGPIIGGSVYARQGVEVLALGNDV
                     DVPTQVAVGVFPPALLEVAKMSDIIKKKREAATKVRNTVQPLLAQLKRLNPQQREKAT
                     ELMYEADRLEEEANALNAKKDEILRPFIPSGDREVSILVNRMVNCRVSIVFGDKVATI
                     LKPRRGPVKFVSRVVDRVESILMVDAVSGSTQILPSQPYEPEPSATEADTKSPGNNEN
                     EPAASQKATRPVAPAQP"
     gene            complement(1258..2124)
                     /locus_tag="P5316_18845"
     CDS             complement(1258..2124)
                     /locus_tag="P5316_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87073.1"
                     /translation="MPVETLLISGPRGGGKSTLARLIASQVLDRPAHYLRTRRSADGY
                     TNAIIPLDPANDGFATGWASCFMVGYTPERVFETLPDGLREVRKIDRYGFAIIEADGD
                     PPLRHAYPYDYRVFVMSPPTDVHQVFRDTREATQALQQVMQDTAAFASEIFGLFNAAG
                     FDDGVGVSHHEPVVIDPCQVAVERLEIAESQIRGFLNSPLGVEIASRIQLQPEYHALV
                     ESDVVVINTGVGKGGEVMKECVKRIEKLLARVRHDARRSSILYWGNFLDTKDSTQKKL
                     LKRLRCLFAMKS"
     gene            complement(2541..3554)
                     /locus_tag="P5316_18850"
     CDS             complement(2541..3554)
                     /locus_tag="P5316_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRR87074.1"
                     /translation="MRKKRVLAIMHKDLVPPDSIEGLTEAEVAPFKTEYDVIAACRSL
                     GHEVRSLGIQSDLSVLGKTIEEWKPHIVFNLLEEFDGEAVMDHNVVSYLELLKLPYTG
                     CSPRGLILARDKGLSKKILSYHRIRVPAFAVFPRRKAVKRPSRLKFPLFVKSLIEEAS
                     LGIAQASIVDDDEKLRERVAFIHERIGTDAIAEQYIEGREFYVSVLGNRRLEVFPIWE
                     LKFTKAGNGVPLIATAKVKFDLEYQKKLGVETSEAKDLAPEVVKGILRLCKRIYRNLG
                     LKGYARIDMRMTADGQVYVLEANPNPQLARGEDFAESARAVGISYERLVQRIMNLGFS
                     RAR"
     gene            complement(3558..4529)
                     /locus_tag="P5316_18855"
     CDS             complement(3558..4529)
                     /locus_tag="P5316_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HRR87075.1"
                     /translation="MRLCDLGVSLSRRPIKRCIERIHQELEQRQIRFRPHFWLGEEWF
                     TPDGIPGAAIPFYLAHPRLARLERSQMLEAEGGTYKWCMRILRHEVGHAIDHAYLLHR
                     RRRWQQLFGKSSQPYPERYVPKPNSKRYVLHLDHWYAQSHPDEDFAETFAVWLRPKAW
                     WRKRYQGWPALKKLEYVDELMREIAGKPPVVTSRKVIEPLSRIRKTLREHYEQKRARY
                     GEGLPAFYDRDLRRLFCGTGDHPRRESAASFIRRHRAEVRRIVSRWTREYQYTLDLVM
                     REMIARCQELNLYVQGPGEQVKMDFAILLTVETMNYIHSGRRRLLIM"
     gene            complement(5326..6759)
                     /locus_tag="P5316_18860"
     CDS             complement(5326..6759)
                     /locus_tag="P5316_18860"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HRR87076.1"
                     /translation="MCAMFHQLDDTIVAVSSPAGTGVRGILRLSGPGAFGLAAGVFTC
                     NDGRSVAELAGHRRVAGRLWMEATASVPAEAYTFRAPASYTRQDVIELHTIGSPAVLG
                     AVLERLTSAGARPAEPGEFTARAFFSGAMDLTRVEGVAGIIHARNDSQLRASEALLHG
                     RLSRRSNELREQLIDLLALIEAEIDFSEEAVEFVAPQRVAEVVSRVYEELALLIHQAP
                     SIERLETLPEVMLVGRPNAGKSTLMNVLTGMDRAIQSAMPGTTRDVLSVPLNVPGGEV
                     MLLDAAGLEAGIGESADEEDRHPDAQAQATARRLMLRAAMLIPVVDATDSPRETLELL
                     RRILPPRPMCVALSKADLVGEIESDRLAAELQGAAPVRVVSAVTGLGLDLLREEIGRT
                     LFAAGESSEADVVMLSDRQRQALRIAHEALGRARHLCEENREVMNAAELLAVEIREAI
                     NGLSVLVGEVATEELLGRIFSRFCIGK"
     gene            6957..>7191
                     /locus_tag="P5316_18865"
     CDS             6957..>7191
                     /locus_tag="P5316_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87077.1"
                     /translation="MEVHGQSDVAKADEVSAATSLQADSVPHDDPLADASCLHCGYSL
                     RGLTENRCPECGAPFDRQEMASSYLPEWPRLMVW"
BASE COUNT         1496 a         2442 c         1906 g         1347 t
ORIGIN      
        1 gcctcattcg acaaccgcag ttgcagaatt cacaccgtca agagcggtga ggtaatcggc
       61 gaatacgttc ccgagacccc gccccagccg ggcatggacg tctacggcaa gcccattccc
      121 ggcgcgcctt ccctcttgcg ttttgaactc ggcgccaacg tcaaactggc ggacgacggc
      181 aagaccgtct acgccacctc caatggcatg gtccgggtca cccggcaggg agtctccgtc
      241 gttgaacagg tcaaagtcga tggcgacgtc gatctcaaga gcggaaacgt cgacgccccc
      301 tctgatgtcc tgatcaccgg caacgtcgtg gaaaccttcc tcgtccgatc ccccaaaagc
      361 ataaccatca agggaatggt cgaagcagcc aacgtcgagg ccggaagcca cgtcatcatc
      421 gccggcggag tcgccggaaa aagcaaggcc ctcattcgcg ccggtggtga gctgcaggtc
      481 aagttcctgg aagaggccgc gatcgaggcg accggcgacg tcatcgtcac caaggaagtc
      541 atgaactgcc gcatccggac aatgggcaga ctcctggtcc ccaggggccc gatcattggc
      601 ggctccgtct atgcccgcca gggcgtcgaa gtcctggccc tcggaaatga tgtcgatgtt
      661 cctacgcagg tcgccgttgg tgtcttcccc cccgcactcc tcgaagttgc caagatgagt
      721 gacattatta agaaaaaacg cgaggcggca accaaggttc ggaataccgt tcagcctctg
      781 ctcgcccaac tgaagcgcct taatccccag caaagagaga aagcaacaga gctgatgtac
      841 gaggccgacc ggctcgaaga ggaagccaac gcgctcaatg cgaagaagga tgagatcctg
      901 cgccccttca ttccctcggg cgatcgcgag gtctccatcc tggtaaacag aatggtcaat
      961 tgcagagtaa gcatcgtctt cggtgacaag gtcgccacga tcctcaaacc ccgccggggt
     1021 ccggtcaagt tcgtgagtcg agtcgtggat cgcgtcgagt cgatcctcat ggtggacgcg
     1081 gtttcgggta gcacgcagat tctacccagc cagccttacg agccggaacc gtcagcaacc
     1141 gaggctgaca cgaaatcacc cggtaacaac gagaatgagc ccgccgcctc ccaaaaagcg
     1201 acaagacccg tcgccccggc tcaaccatag tcccggccga taccggcgcg ccaggcctca
     1261 agacttcatc gcaaataggc atcgcagccg cttaagcagc ttcttctgcg tggaatcctt
     1321 cgtgtccagg aaattgcccc aatacagaat gctcgaacgc cgggcgtcat gccgcacgcg
     1381 cgcaagcagc ttttcgatcc gtttgacgca ttccttcatc acctcgccgc ccttgcctac
     1441 ccccgtgttg atgacgacga cgtccgactc gacgagagcg tgatactcgg gctgaagctg
     1501 aatacgcgac gcgatctcaa cgcccagcgg cgagttgaga aagccgcgaa tctgcgattc
     1561 ggcaatctca agccgttcga ccgcaacctg gcaaggatct atgacaaccg gctcatggtg
     1621 cgacacccca acgccgtcgt caaaaccggc cgcattgaac aaaccgaaaa tctctgatgc
     1681 aaacgctgcc gtgtcctgca tcacctgctg cagcgcttgg gtcgcctcac gcgtatcccg
     1741 aaacacttgg tgaacgtccg ttggcggcga catcacaaaa acgcggtagt cgtaagggta
     1801 tgcatgccga agcggcggat ctccgtcagc ctctatgatc gcgaatccgt atcggtcgat
     1861 cttgcgaacc tctcggagcc cgtcaggcaa agtctcaaac acgcgctcgg gagtgtagcc
     1921 gaccataaaa caggaagccc aacccgtcgc gaagccatcg ttggccgggt caagaggtat
     1981 gatcgcattg gtgtatccgt cggccgatcg gcgcgtccga aggtagtgcg ctggccggtc
     2041 cagcacttga ctggctataa gacgggccaa tgtgctcttt ccgcctccgc gcggaccgct
     2101 gatcaacaga gtctccaccg gcatgtcgtg ttatcccaac ggaccactct tgaaaaacgt
     2161 ggatcccaag agaaagggcc cgatactcat tctattcacg agattatcga ttctccaccg
     2221 ccgaaagatt aagttcacgt ccctattgac ttggccggcc ttttcaaagc cgagactccg
     2281 attcgccgag ccgaccgggc ttgccctgca gaggcccctg gcaaaaccgc aacggagcgg
     2341 ccccttttat ggcgtcaccg cttggcagtc cggccgatcc cctgggcttt cacctccgta
     2401 agcgccaccc gatccctgct cgccccacga tcgcccgtag tctattgccc catatgagct
     2461 taactggatc caacggccgg cctcaaggcc gccatccgca cgagccggca acctgcctca
     2521 ccccgttcga tggcgccaat ctacctcgct cgactgaaac ccagattcat gatccgctga
     2581 acgagccgct cataagaaat gcccaccgcc ctggctgatt cggcgaagtc ctcccctcgt
     2641 gccaactggg ggttcggatt cgcctccaat acatacacct gcccgtccgc cgtcatccgc
     2701 atgtcgatcc gcgcgtaacc cttgagcccc aggttgcggt agatgcgctt acacagcctc
     2761 aaaatcccct tgaccacttc gggcgcgaga tccttggctt ccgaagtctc cactccaagc
     2821 ttcttctggt attccaggtc gaacttcacc tttgccgtcg cgatcagcgg gacgccgttc
     2881 ccggccttcg tgaacttgag ctcccagatc ggaaacacct ccagccgccg attgcccagc
     2941 acactcacgt aaaactcccg cccttcaatg tactgctcgg caatcgcgtc cgtgccgatc
     3001 cgctcgtgga tgaaagccac ccgttctctg agcttctcgt catcgtcgac gatcgaggcc
     3061 tgcgcaatgc ccaacgacgc ctcttcaatc agcgatttca cgaacaacgg aaacttgagg
     3121 cgactcggcc gcttgacagc cttacgcctc ggaaaaaccg caaaggccgg tacccggatg
     3181 cgatggtaac tcaggatctt cttcgacagc cccttgtctc gggccaggat cagacctcgc
     3241 ggcgaacagc cggtatacgg cagcttgagc agctcaaggt agctcaccac gttatgatcc
     3301 atcaccgcct cgccgtcgaa ttcctcaagc agattgaaga caatgtgcgg cttccactcc
     3361 tcgatcgtct tgcccaggac gctcagatcg ctctgaatgc ccagcgaccg cacctcatgt
     3421 cccaacgacc ggcacgccgc gatgacgtcg tattccgtct tgaacggtgc aacctcagcc
     3481 tcggtcagcc cctcgatgct gtcgggggga accagatcct tgtgcatgat ggccagaacg
     3541 cgcttcttcc tcatggctca catgatcagc aatcgccggc ggccgctgtg aatgtagttc
     3601 atcgtctcaa ccgtcagcag aatggcaaaa tccatcttca cctgctcgcc cggaccctgc
     3661 acgtacaaat tcagctcctg gcaccgcgcg atcatctctc gcatgaccag atccaacgtg
     3721 tactggtact cccgcgtcca ccgcgacacg atccgccgga cctcggcacg atggcggcga
     3781 ataaacgaag ccgccgactc acgccgcggg tggtcgcccg ttccacagaa gagcctgcga
     3841 aggtcccgat cgtaaaaggc cggcagccct tctccgtaac gggcccgctt ctgttcgtag
     3901 tgctcgcgca gcgtcttgcg aatcctcgac aaaggctcga taaccttccg actcgtgacc
     3961 acgggaggct tccccgcaat ctcccgcatc aactcgtcga catactccag cttcttgagc
     4021 gccggccagc cctggtacct ctttcgccac catgccttcg gccgaagcca gacggcaaag
     4081 gtctcggcaa aatcctcgtc cggatgactc tgcgcatacc agtgatcgag atgcagcacg
     4141 taccgcttgc tgttcggctt gggaacgtaa cgctccggat acggctgcga tgatttgccg
     4201 aaaagctgct gccagcgtcg acgacgatgc agcagataag catggtcgat cgcgtgaccc
     4261 acctcatggc gaagaatccg catgcaccac ttgtaagtgc ccccctcggc ctcgagcatc
     4321 tggcttcgct cgagccgcgc cagcctcggg tgagccagat agaagggaat agccgcgccc
     4381 ggaatcccgt ccggagtgaa ccactcctca cccagccaga agtgaggccg aaaacgaatc
     4441 tgccgctgct ccaattcctg gtggattcgc tcgatgcaac gcttgatcgg cctccgcgac
     4501 agactcaccc caaggtcaca taaccgcatg tccaggagcc gatcctcggg atagttgctc
     4561 cacacgtgcg gcggaatcgg cccgggcttg ttcccacgct tgcgcttcga gaaacctgat
     4621 tgcggcacca tcgttcgatt tgtcccgact tgcggcacgg ccggtatcaa cgggaaccat
     4681 cgacaaccac gccgcccgct gaccagatat atcggtcctc catcaaagga tcgtaacacc
     4741 ccgtccccca acgtgcgaat cagcagcgca attcactgat cggccggtaa acttccgctc
     4801 atccggacgc aacggctcgc ggcttgttcg ccacggcaca accgacgcac cggagaacgg
     4861 tccccccgcc ggccggcctt acacaaccgg cgcctttccg tccgtcgacg ccgttttcag
     4921 tactgcccga agcattcttc catggcgcat aaaaatggga agtaccacgc cgtggcgagg
     4981 ggtacttccc acatggtgtt gtcgttcttc cgtactcagc gaagagacga ccgccgcagc
     5041 ctcgcactga tggggccgtt cacggcacgc actgtcaggc gccagccgcg gctgatgctt
     5101 gcttcggctt atttcttctt gcgagccggc ttcttggcct tcttcgcagc cttcttggct
     5161 ttcttctttg ccaccgaagt atctcctttc tggtagcagg cccaaacgaa catctcaacg
     5221 cttgtcaaag agcattggcg tttcctgaga ttttcctgca aaaaatcctc aagtttcaac
     5281 gccgttcgtt atatcgacca gttgcaccgg gtctctttac tattttcact tgccgatgca
     5341 gaagcgagag aagatccgac ccagcagctc ctcggttgcc acctccccca ccagcaccga
     5401 cagcccgttg atcgcttcgc gaatctcaac cgcgagcagc tcggcagcgt tcatcacctc
     5461 cctgttctcc tcgcacaggt gacgcgcacg acccaatgcc tcatgggcga tccgaagcgc
     5521 ctgccgctga cgatccgaaa gcatcacaac atccgcctcg ctcgattcac ccgccgcaaa
     5581 cagggtcctc ccgatctcct cgcgaagcaa atcgagaccc agccctgtga ccgcgctgac
     5641 aacccggacc ggtgcggctc cttgcaactc tgccgccaga cgatccgact cgatctcccc
     5701 gaccagatcg gccttgctga gcgcaacgca catcggtcgc ggcggcagaa tgcgccgcag
     5761 taattcaagc gtttccctcg gcgaatccgt cgcgtccacg accggaatca gcatggcggc
     5821 tcgcaacatc aagcgacgag ccgtggcttg tgcctgcgcg tccggatgcc gatcctcttc
     5881 atcagccgat tcaccgatgc ccgcctccag gcccgccgca tccagcaaca tcacttcgcc
     5941 ccccggaacg ttcaacggaa ccgagagcac gtcacgcgtc gtaccgggca tcgccgactg
     6001 aatcgcccgg tccatgcccg taagcacatt catcagcgtg ctcttccccg cgttgggccg
     6061 gcccacgagc atcacctcgg gcagggtctc aagccgctcg atggaggggg cctgatgaat
     6121 cagcaaagcc agctcctcat atacgcggct caccacctcc gctacccgtt gcggcgccac
     6181 gaactcaacc gcttcttcgg agaagtcgat ttccgcttcg ataagagcca gcagatcgat
     6241 cagttgctcg cgcagctcat tggagcgtcg actcaaacgg ccgtgcagga gtgcctccga
     6301 agcacgcaac tgcgagtcat tgcgggcatg gatgatcccc gcaacaccct cgacgcgagt
     6361 caggtccatc gccccggaga aaaacgcccg ggcggtgaac tcgccgggct cggccggccg
     6421 tgcgccggcg ctggtcaagc gctccaaaac cgccccaagc accgccggcg agccgatggt
     6481 atgaagctcg atcacgtcct ggcgcgtgta gctggcgggc gcacgaaacg tgtatgcctc
     6541 cgcgggcact gaggccgtcg cctccatcca caatcggccg gcgacccgac gatggccggc
     6601 caactccgcc acagatcgcc catcgttgca agtgaacaca cccgcggcca agccgaacgc
     6661 acccggcccg ctgagccgta ggatcccccg cacgccggtc ccggccggag acgaaacggc
     6721 cacaatggtg tcgtccaact ggtggaacat cgcacacatg atacgcgatc ggatggggag
     6781 tctgttagtc tattggtctg ttagtctgcc cgtctgttgg ggtgccatac tcccgggatc
     6841 cctgcctgct gaaccgttgc gtctgggacg gactggggat ctcaaattgg agatttcaga
     6901 ttgcggacgg aattgaccgc ccttagcata ctggccgggc cagacggata ccttcaatgg
     6961 aagtacacgg gcaatccgat gttgcgaaag ccgacgaggt gtcggctgcg acatcattgc
     7021 aggccgattc cgtcccgcat gacgaccccc tcgccgacgc atcatgcctt cactgcggct
     7081 actccctgcg cgggctcaca gagaatcgct gcccggaatg cggtgctcca ttcgaccggc
     7141 aggagatggc gagcagttac ctgccggaat ggccccggct gatggtctgg t
//
LOCUS       DAOTPW010000277         7083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9242_length_7083_cov_4.856858, whole genome shotgun sequence.
ACCESSION   DAOTPW010000277 DAOTPW010000000
VERSION     DAOTPW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9242_length_7083_cov_4.856858"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..479)
                     /locus_tag="P5316_18870"
     CDS             complement(201..479)
                     /locus_tag="P5316_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRR87078.1"
                     /translation="MTANAIAFLSLPGGPEWIIIGVVGLLIFGKKLPEVARSLGKSVV
                     EFKKGLKAVEDEITSAGNESDNPSNQLPPTSNPYSSESAAEPPPNQNS"
     gene            1153..1659
                     /locus_tag="P5316_18875"
     CDS             1153..1659
                     /locus_tag="P5316_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87079.1"
                     /translation="MTCGRTLGVCAGLIVAGLGGWAILDLAANAQTLPANAPAEAKSS
                     VRLPEPQNVTLTGRLVDVHSFMTDKYPDADRGKTTADRLKAGVPAGLDTPAGLILLGT
                     GAKYSPDKLIPLAYQEVEVKGKRYYRRGVWYLEFTSINKAKAADTTGLPAKPATVRDQ
                     PASAPASS"
     gene            1790..4591
                     /locus_tag="P5316_18880"
     CDS             1790..4591
                     /locus_tag="P5316_18880"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87080.1"
                     /translation="MGNNRRTIRVCWCAGFLCYLAGGTAAAGTWVADNAGLRHLTVIG
                     IGISPSNPGVIYIQTRSLGIYKTTNGGQVWSKTATFNGDKGPGFDHLLHQGPAVHPTD
                     PNTVWVASGGQVYKTTNGGASWFLSSSGTTLSGICNGVQGVVVDPNDANHLFAGTIIS
                     GCDGGVFESTNGGANWTNIAGSLAGGGVGNDAWPIALDASDVSRLYCGSPHNSVYRSI
                     DGGYTWVNSPPVTGDASSYEVVINPLSPNKIWAGAAGGTWLSADYGVTWARQTQSFNN
                     MLIAAIRFAPSNPQVVYAISGNKIWRSLDNGATWTAGPVLLGGARAIEVDPADANVVY
                     VGTWGLGMYKSVDGGQTFMEINTGLPLTSLFYGLQAFAVGDVRYCILSGDVIYRRLPG
                     EANWECYSMSPGDFVRVDRYRPNRWYSAALQGGLWRSVDFGLTWQEIYPETGPTEVFG
                     FWPDPRTCNRIWIGDSDGSKVLRSDDGGDTWTQAGTIPAVPKGFTVFSDLAGDPFDPN
                     VVLAAAWPTYSSNKQHGYVWRSADGGRTWAHVREGMFYGDWRIGDGDWYISNYAMHQR
                     INVYCNYRVNLDHHTFGNGSYRCRMRIISSYNNDPVNWVGFSIRLSDVDSHWGSSGWL
                     VFMRRNGEVALWNQTDKTVLSSVAVADPAQWTTIRLDAWGNQFELFVNETSIGTYTDP
                     NHRWDGPGYFGLVTCKTESDYDDVFIAAETNYADDFGVSVQYPASMGRWLAADAHNPG
                     VFALSTQGAGVWRTADHGATWERITDTAGEGKVNYRPMISSGYSGNIYVCRGSGYSWS
                     IDNFHSQGAVRQKIGQTLSYSSLVLGEDWLNPQRLFAAVYAQGILVYEAGDIIGEPAS
                     PIPVRMDFDKDGDVDQADFGRMQACLTGVGVLQMDPLCDRTLLDHDDDTDADDLGIFR
                     DCLSGPGLEPDPACDCSAP"
     gene            4886..6331
                     /locus_tag="P5316_18885"
     CDS             4886..6331
                     /locus_tag="P5316_18885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR87081.1"
                     /translation="MSSFENTRLEKDIYQSRPFWRWLALIAALLAWGFDGVEQGVYTI
                     MTREALKDLIPQISDLVAQANDLKTQIDGVPESAQGELLGRLEPLTKQIDEVVGPKFS
                     LSLAMWMWGAAAGGVLFGRLGDRFGRVRSLLLSVSMYAAFTGLSALSGHWWHFIACRF
                     FGAMGLGGTWPLCVALVVETWPERLRSVLAGAIGAAANVGYFVAATYSRLMVGYSWRW
                     VIGMGFFIGISSLPLIFFVPEPTQWRVSRAKKEHSSLGDLFSRSYRRSTVVGSLLSTV
                     ALLGTWGSFLWLATYVDQITEGTTHHGSGRTIVAQWAAYGQMVGGFMGGLAAGWLGNR
                     RSWLLLCFGAWATVSGLFFFNTHFSWLMLLMGTLAGLFVTGFFGWLPKFLPELYPTRI
                     RASGQGFCYNIGRVLTGLGVFGAGYLVSAFGGDYRPAAMTIATVYLLGLLVIWFAPDT
                     GGKMQEDGVGSPLGGPVPELERAAAGREESG"
     gene            6328..>7083
                     /locus_tag="P5316_18890"
     CDS             6328..>7083
                     /locus_tag="P5316_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87082.1"
                     /translation="MKTTKLTDVRAVGASLYFLPVRTRVPLKFGPETLTYVTCARACV
                     KVRDAAGRTAEGWGETPLSVQWVWPSGLTYDRRHETLKRFCGMLTKAWAEFDVSGHPI
                     EVGHSFQRDVLPVLLAAINREYLDPSEHMPWLAALVCCSPFDIALHDALGRLVDRPVY
                     QTYSGEFMNRTLAEFLEPAVGVKVRFSGRYPADYLIADPKKRLAAWHLVGGLDPLDAS
                     ELSGSEPEDGYPVLLLDWIRRDGLKCLKVKLRGN"
BASE COUNT         1250 a         1951 c         2443 g         1439 t
ORIGIN      
        1 cgatccagaa acatgcgcgc aagccgcaca gatatctggc cgtaaggttc taaggagcta
       61 tgcgaattgg aggtaggcag gagctggtac cgccgggcag gcgaagcgga gttctcactt
      121 gccgcccggt gtggggagtt cggcggacca gcatgttcta agccgccagt ggtgggcgaa
      181 cgagcacgtc gggtgggctg tcaggagttt tggttcggcg gcggctcggc tgcggattcc
      241 gaactgtagg ggttgctggt cggaggcaac tggttcgagg ggttgtcgga ctcgttgccg
      301 gcgctggtga tctcgtcctc gacggccttg agccctttct tgaattcgac cacgcttttt
      361 cccaacgaac gagcgacctc aggcagcttc ttgccgaata tcaggaggcc caccaccccg
      421 atgatgatcc actcgggccc gccggggagg ctgagaaagg ctatcgcgtt tgccgtcata
      481 gggactgctc cacgctcggg gtggcggcgc ggacgcggct actgcctggg acccagccac
      541 cccgaggctg ttgagttttc tccagatgga ccattgtaac ccgagcgcga cccaaatgat
      601 acaccaggtc tcgccctgac cgacgagtgt ggcgttgcaa gggctgtacg tctgcagcgc
      661 atgaccaaga cacaagtcca ggtgaatgac gaaatacgaa tgacgaaaga gactcgcctg
      721 caggcctatg tccagacacg gtttatggat gccgcgtcag agagcctatg ccccgggtaa
      781 cacgggcaag gtacgggaga ggcgtggcgg gcctgcggcc agcgtgcggg ctggcacctg
      841 gcggctggcc gaaggggagt gtcgccacga agcctgtggg gaaaagggca cattggcggg
      901 gtgagcggcg gtttcccgtg cttggagtcg ttcgtttggc gggattccga ctcatggcct
      961 acaggcagga cgatcggtac cggtcgtttg gatggttcag atcacttaaa acattgccga
     1021 ttactaggtt gcgcaattcg ttgtttgggt acggtcctgg tgtgaagcca agcgcccggc
     1081 gctcgtatac cagcgtgtca acgagatgag gcagttgacg gcgagcgcct cggctgtaag
     1141 ggagacgaca tcatgacgtg tggcagaacc cttggcgtgt gtgcgggtct catcgtggcc
     1201 ggccttggtg gttgggcgat tctggatctt gcggcgaatg cgcagacact tccggcaaac
     1261 gccccggccg aggccaagtc gtcagtgcgc ctgccggagc ctcaaaatgt gaccttgacc
     1321 ggtcgtctgg ttgatgtgca cagcttcatg acggacaagt acccggatgc cgaccgcggc
     1381 aagactacgg cggatcggct caaagccggt gtgccggcgg gtttggacac gccggccggt
     1441 ctgatcctgc tcggcactgg ggcgaagtat tcgccggaca agcttattcc cctggcttat
     1501 caggaggtcg aggtcaaggg taagcgttat taccgacgcg gcgtgtggta cctggaattc
     1561 accagcatca acaaggccaa ggcggcggat accacgggtc tgcccgccaa accggcaacg
     1621 gttcgtgatc aacccgccag cgcgccggcg agttcttgat ctttcctcga ctggtgcgat
     1681 cagcagccat ggggtgtgga ggccttggtg ggcatgggca aggtctgccg ggacgagacc
     1741 gggttgtggt atattggtgt catggtcgcg gggagcggag gagaggctgt tgggaaacaa
     1801 tcgtcgaacc atccgggtat gctggtgtgc gggtttcctg tgttacttgg cgggcggcac
     1861 ggcagcggcc ggtacgtggg tggccgacaa cgccggtctt cggcatttga ctgtgatcgg
     1921 gatcggcatc tctcccagta atcccggcgt catctatatc cagactcgat ctcttggaat
     1981 ctacaagacc accaacggtg gacaggtctg gtccaagacc gcgactttca acggggacaa
     2041 ggggccgggg ttcgatcatc tcctgcacca gggaccggcc gtccatccga ctgatccgaa
     2101 cactgtctgg gtggccagcg gggggcaagt ctacaagacg accaacggcg gggcctcgtg
     2161 gtttctctcg agcagcggca cgacgctgag cggaatctgc aacggtgtgc agggcgtcgt
     2221 ggtggacccg aacgacgcga atcatttgtt cgccggtacg atcatctcgg ggtgtgacgg
     2281 cggggtgttc gagtctacca atggcggggc caactggaca aacatcgccg ggtcgcttgc
     2341 ggggggcggg gtgggcaacg acgcatggcc gattgccttg gacgcatccg acgtcagtcg
     2401 cctttattgt ggttcgccgc acaactccgt gtaccgctcg atcgatggcg gttatacgtg
     2461 ggtcaactca ccgccggtga cgggcgatgc gagcagctac gaagtggtca tcaatccgct
     2521 ttcgccgaac aagatctggg cgggtgccgc cggggggacc tggctgagcg cggactacgg
     2581 cgtgacctgg gcgcgccaaa cacaatcgtt caacaacatg ctcattgccg cgattcgctt
     2641 tgccccgtcg aatccgcagg ttgtctatgc catctcgggg aacaagatct ggcgcagtct
     2701 cgacaacggc gctacgtgga ctgccggccc ggtgctgctc ggcggggcga gagccatcga
     2761 ggtcgatccg gccgacgcga acgtggttta cgtcggcacg tggggtctgg gcatgtacaa
     2821 gagcgtcgac ggcggccaaa ccttcatgga gatcaacacc ggtctgccgc tgacgtcgct
     2881 gttctatggg ttgcaggctt tcgcggtggg agatgtgcga tactgcattc tcagcggtga
     2941 tgtcatttac aggcgtttgc cgggggaagc caactgggaa tgctattcca tgtcgccggg
     3001 cgatttcgtg cgcgtcgacc gataccgccc caaccgctgg tacagcgcgg cattgcaggg
     3061 tgggctttgg cggtcggtgg atttcgggct gacgtggcag gagatttacc ccgagaccgg
     3121 gcctaccgag gtattcggct tctggccgga cccgcgaacc tgcaaccgga tctggatcgg
     3181 ggacagcgac ggcagcaagg tgctgcgaag cgacgatggc ggcgatacct ggacgcaggc
     3241 ggggacgatt ccggccgtcc ccaagggttt caccgtgttt tccgacctgg ccggcgaccc
     3301 atttgacccg aacgtggttt tggccgctgc ctggccaacc tacagcagta acaagcagca
     3361 cggctatgtc tggcgttcgg cggacggcgg ccggacgtgg gcgcacgtgc gcgaggggat
     3421 gttttacggc gactggcgga tcggcgacgg tgattggtac atttccaatt acgcgatgca
     3481 ccagcggatc aacgtgtact gcaactatcg ggtgaacctg gatcaccaca cgttcggaaa
     3541 cggctcgtac cggtgccgga tgcgcatcat cagttcttac aacaacgatc cggtcaactg
     3601 ggtcggattc agcattcgcc tgagcgacgt ggacagccac tggggttcgt ccggatggct
     3661 ggtctttatg agacgcaacg gcgaagtcgc cctctggaac caaaccgaca agacggtttt
     3721 aagctcggtc gcggttgcgg accctgcgca gtggacgacc attcggctcg atgcatgggg
     3781 caaccagttc gagctgtttg tcaacgaaac ctcgataggg acatacacgg acccgaatca
     3841 ccgttgggac ggtccggggt acttcggatt ggtgacgtgc aagaccgaat ctgattacga
     3901 cgatgtgttc atcgccgccg agaccaatta cgcggatgat ttcggtgtat cggtgcagta
     3961 tccggcgagc atgggccgct ggcttgcggc ggacgcgcac aacccggggg tttttgcctt
     4021 gagcacccag ggagccggtg tgtggcgcac ggccgaccac ggggcgacat gggagcggat
     4081 caccgacacg gccggggagg gaaaggtcaa ctaccggccg atgatctcca gtggctacag
     4141 cggcaacatc tacgtctgtc gcggatcggg ctattcgtgg tcgattgaca actttcacag
     4201 ccagggcgct gtcaggcaga agatcggcca gaccctgtcc tacagcagtc tggtgctggg
     4261 cgaggattgg ttgaacccgc aacggctttt tgcggccgtg tatgctcagg ggattctggt
     4321 gtacgaggcc ggtgacatca tcggcgagcc cgcatcgccc attccggttc ggatggactt
     4381 cgacaaggac ggcgacgtgg accaggctga tttcgggcgc atgcaggcat gcctcaccgg
     4441 agtgggcgtg ctgcaaatgg atccgttgtg cgaccgtaca ctcctggatc acgatgacga
     4501 cacggacgct gatgatctgg gcatctttcg cgactgcctg agtggtcctg gcctcgagcc
     4561 cgatccggcc tgtgactgct cggcgccgtg aaaccccgca gacactccag acagactgat
     4621 ccttgaccgc ttcaagacga tcctctatca cctaaggcgg accgcctttt tgcgggccgc
     4681 agcccaaggg ctctgtgatc cggaaacacg cgcgcaagcg gcacagacgt ctggccgtaa
     4741 ggattgaaag gcgcatgccc acgcaaagcc gtgggcatgg cacccggcgc actgcgatcc
     4801 ggaaacatgc gcgcaagccg cacagatgtc cggccgcaag gttctaaggt tgtcggcatg
     4861 atcggtgcat atgaggaaaa cctgcatgag ctctttcgag aacacgcggc ttgagaagga
     4921 catctaccag agtcggccgt tctggcggtg gctggctctg atcgcagctc tcttggcgtg
     4981 gggtttcgac ggggttgagc agggcgtata cacgatcatg acccgggagg ccctgaagga
     5041 tctgattccg cagatctcgg acctggtggc tcaggcaaac gatctgaaaa cacagattga
     5101 tggcgtgccg gaatcggccc agggcgaact gctcggtcgg ctcgaaccgc tcaccaagca
     5161 gatcgacgag gtggtcggtc cgaagttcag cctatcgctg gcgatgtgga tgtggggggc
     5221 ggcggccggg ggcgtgttgt tcgggcggct gggtgatcgc ttcgggcgcg ttcgatcgct
     5281 gctgttgtcg gtatcgatgt atgcggcgtt cacgggtttg tcggcgttgt ccgggcactg
     5341 gtggcatttt atcgcctgtc ggttcttcgg agcgatgggt ttgggcggca cgtggccctt
     5401 gtgcgtggcc ctggtggtcg agacctggcc ggagcgactg cgttccgtgc tggcgggggc
     5461 catcggcgcg gcggccaacg tgggctattt cgtcgcggcg acctattcgc gtctcatggt
     5521 gggttacagt tggcgatggg tgatcgggat gggttttttc atcggcatca gcagcctgcc
     5581 gctgatcttt ttcgtgcccg agccgaccca atggagagtc tcgcgggcca agaaggagca
     5641 ttcgtcgctc ggagacctgt tctcgcgcag ctatcgtcga tcgacggtgg tggggagcct
     5701 gctgtccacg gtggcgttgc tggggacgtg gggcagcttc ctgtggttgg cgacttacgt
     5761 cgaccagatc acggaaggaa cgacccatca cggcagcggc aggacgattg ttgcgcaatg
     5821 ggcggcgtac gggcagatgg tcggcggctt catgggcggg cttgcggcgg gctggttggg
     5881 gaatcgccgg tcgtggctgc ttctgtgttt cggcgcctgg gcgaccgtca gcggcctctt
     5941 tttcttcaat acgcatttca gttggctgat gttgctgatg ggcacactgg ccggcttgtt
     6001 tgtcaccggc tttttcggct ggctgccaaa gttcctgccg gagctctacc cgacgcgcat
     6061 ccgggcgagc gggcagggtt tctgttacaa catcggccga gtgctgactg gtctgggagt
     6121 gttcggggca ggctacctcg tgagtgcgtt tggcggcgac taccggccgg ccgccatgac
     6181 catcgcgacg gtttatctgc tgggtcttct cgtcatctgg tttgctccgg acaccggcgg
     6241 caagatgcaa gaggacggcg tcgggtcacc actcggtgga ccggtgcccg aacttgagag
     6301 ggcggccgcc gggcgggagg agagcgggtg aagacgacaa agctcacgga cgttcgcgca
     6361 gtcggtgcat cgctgtactt tcttccggtt cgaacgcgcg tgccgctgaa gttcgggccg
     6421 gagacgctga cctatgtgac ctgtgctcgc gcatgcgtga aggtgcgtga tgctgcgggg
     6481 cgaacggccg agggttgggg cgagacgccg ttgagcgtgc agtgggtctg gccgagcggg
     6541 ctgacttacg atcgccggca cgagacgctc aagcgattct gcgggatgct gacgaaggcg
     6601 tgggcggagt tcgacgtttc gggccatccg atcgaggtgg gtcactcatt tcagcgggat
     6661 gtgttgccgg ttctgttggc cgcgatcaac cgtgagtatc tcgatccgtc ggagcacatg
     6721 ccttggctgg cggcgctggt gtgctgctcg ccgttcgata tcgctctgca tgacgcattg
     6781 ggcagactgg tcgatcggcc ggtgtatcag acctacagcg gcgagttcat gaaccggacg
     6841 ctggctgagt ttctggagcc ggccgtcggc gtcaaggtca gattctcagg acggtatccg
     6901 gctgattacc tcatcgcaga cccgaagaag cgacttgccg cctggcactt ggtcgggggg
     6961 cttgatccgc ttgatgcttc tgaattgagc ggttcggagc ccgaggacgg ctatccggtt
     7021 cttctgctgg actggattcg ccgcgatgga ctgaaatgcc tcaaggtcaa gctccgcggc
     7081 aac
//
LOCUS       DAOTPW010000278         7058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9281_length_7058_cov_5.430673, whole genome shotgun sequence.
ACCESSION   DAOTPW010000278 DAOTPW010000000
VERSION     DAOTPW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9281_length_7058_cov_5.430673"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            799..2220
                     /locus_tag="P5316_18895"
     CDS             799..2220
                     /locus_tag="P5316_18895"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR87083.1"
                     /translation="MIVGSHRTSCGVGFVALAMSLNGCAHPQSLPVRVLLISDRDTCA
                     CSRFADRLTGRGEVQIHLVESLDGISSSAKTDVLLLDWASPIELDDVTERNIVQFVRS
                     GKGIVAMGYSLKGVCSCPALSGILGGSAKSTMRSGDVSFVVLDPADATMLGIGGGLAW
                     VDELPIINPSITRDNNVLIRTAEPPAFPGDGIAGKPVPVAWTRRVGEGRVFATVLGCE
                     DAVLGDERFITIIHNALRWCGGRMADTRHNVLSVPEQQAGFELLFNGRDLTGWTGDPK
                     LWSVENGEIVGRGEKLPHNVFLIHDKTYSDFVLRYSVRLVNHNSGVQFRSQRFPEFVV
                     KGYQADIADRWYGSLYEEGLGRGVLADGFAGKGEKVARIDGWNDMAVKAVGTRITIKL
                     NGLTTADFTETDPNRPGSGVIALQLHAGPPMEVRFRDIRIRPLAEAAKEQDSLKPFRP
                     GIAERASGSFPCPAVPAFRRSAG"
     gene            complement(2132..3607)
                     /gene="gltX"
                     /locus_tag="P5316_18900"
     CDS             complement(2132..3607)
                     /gene="gltX"
                     /locus_tag="P5316_18900"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRR87084.1"
                     /translation="MSSTSPARVRFAPSPTGYLHIGGARTALFNWLIARQTGGTFILR
                     IEDTDTARNVDGADQKIMEDLRWLGLQWDEGVGVGGPNGPYYQSQRLEYHRSVVNRLL
                     ESGNAYYAFDTAEELEAMRAEAEAKKQAFMYPRPATFPDESDVRKARQTGRPVVVRFK
                     APGQDVTVHDEVMGDVTVSARELDDFIILKADGMPTYHLANVADDAAMGVNFVLRGQE
                     FLSQTPRHIALQRALGFTTPRYAHLPLIMDMQGRKLSKRDGAVEVFAFRQAGYLPEVL
                     VNFIALLGWSPGRDREKMTLQEMIELFSIDRIGRTNARFDRDKLIAFNTDAGASASEE
                     RLAVAFDDYLAVNPDLRISRAKLDEATRRSLLRINKGFRTFADIEYKCGFIYDADENI
                     AYDPDAIKKVLTKGDNAGYRMLESLVPRLESVDPWTAENVESLLKQICQEQSVNMGKV
                     AQPIRVAVSGSTISPPIGETLALLGKEKSLKRVRQCLAGRA"
     gene            complement(3604..4587)
                     /locus_tag="P5316_18905"
     CDS             complement(3604..4587)
                     /locus_tag="P5316_18905"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HRR87085.1"
                     /translation="MLKPVTRESAAIERRAARKRRRLRTIGMLPTLLTLGNLCFGFAA
                     IHSCGRDLEEIHAPRSPDVALTFRRELLQKYAPSFLSFASWMIVGSLLCDALDGRVAR
                     KTGQASKFGEQLDSLADIVSFGVAPAVMMVTLVHREITQWGYAPLQFQHFGRLTLFVG
                     IIYACCAALRLARFNVEASLDEAAHQGFKGLPSPGAAIAVISVVFLHESIDASSEWAQ
                     SANFLTKVLPFCTLVLALLMVSRLRYAHAANWLLRRRPLEHVILILLAFPLIWIYTEL
                     SLLVIAWAFALSGPARYVAGRLTGRAGRAAAAPATGLVPPSTDTEADKKAL"
     gene            complement(4591..5295)
                     /locus_tag="P5316_18910"
     CDS             complement(4591..5295)
                     /locus_tag="P5316_18910"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRR87086.1"
                     /translation="MRIPLAPAGCREMIILTLVLACPAASFYVVAASGHSWAWFAGGL
                     ATVLWLGGMAFFRDPQRQTPTVTGILVAPADGKVVEVSNLEGHPDVGSPATRIGIFLS
                     VFDVHVNRSPCSGTVRRITYQPGQFLDARDPDSGRLNEANTIVIEPDDPSKGPVVVRQ
                     IAGLIARRVVCAVKVGDRVETGQRIGLIKFGSRTELIIRTDSGYAPAVNVGDRAYGAV
                     TVVARQANSRSIAKES"
     gene            5575..6207
                     /locus_tag="P5316_18915"
     CDS             5575..6207
                     /locus_tag="P5316_18915"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR87087.1"
                     /translation="MAKQTPVSAQPGTINVEDALLVEQVRAGDMSAFSRLVARYQDRI
                     VNTCWRVSGNHDDAQDLAQEAFLRALERIDSFEQRAGFYTWLFRIAVNLALSHRRKAA
                     QVVKLSLHGSDGGMRTEHQAARLVGRVTREADDPSAKVASREAGRLVAEAIEQLDDES
                     RAVVVLRDIEGFDYQQIAEMLDLPMGTVKSRLYRARMELRGRLGPELSPG"
     gene            6240..>7058
                     /locus_tag="P5316_18920"
     CDS             6240..>7058
                     /locus_tag="P5316_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRR87088.1"
                     /translation="MNAERREYLEMQLSAYLDGELTPQEQAEVEAWLAADPEARRLLA
                     QLRATVEAVQSLPRARASEELLGNLRTRLERKALLDAPGPAEVVTRTPTPSWGKWVAA
                     AAVIALTVTAGYFTWTLRSEQQVGGTQLASRDRKEAAEPEFSAATERTKQDIAPEELF
                     KHREAGDTTLAAGPAVSPPASASPARKPSERAPGGAPALPAPGKPLASLNEGESVRAE
                     QGKKGDSLAEAPAARGAVADALIGGAEKDVLGDKKPDMLSFAGRPEGRSAESGGI"
BASE COUNT         1341 a         2086 c         2259 g         1372 t
ORIGIN      
        1 cggcaaagtc ccaggtcatc tctacgcggt ggtgcgaatc gttcctccaa agtctccgac
       61 gcctcagcaa cgcgagctat ttgagagact ccgccagacc gagcaggagt cgccacgagt
      121 cggcctgggc tggtgacgcg atccatctcc tcgcgcgtca cgatgttgtc gcccgcggtt
      181 ctctcgtgac cggctatcgt cgcgttttcc tttcctgttt ctcaatgcgc gcaaaaaaac
      241 gcccgcgcgt tttaagggga ggttagagtc gcggagagcg cgggcgcaag ggaatggctt
      301 ttttgtctgg ggcgtctttg cgcgcgcccc gtcacccgcg gctgaatttg agaccgccgc
      361 ggatcggtct ctgtcgtcct ccaaagcgag actgcgcttc cctcttcttc agactgctcg
      421 tgtcccccgg tttcggttcc tacgctactg gggtgatttg tctgttgcat ggaaccgaaa
      481 ccggacgggg ctatccaccc caccgaccac gagtttatca caactgactc gtgtcttcca
      541 cacgttgtga ggcggttttc tgtttcgagc gcgggtcatt tgatctgaaa cggcgactgc
      601 ctgtttcaag tcggcgattc gtcaatcatt ggtgtttgac ggttcacaag acattgctcc
      661 gaggctgctg atatggtctg actgatatca cgacatgctc aggtatacaa tcaagcggtt
      721 cgcagcttgc actgcgggtt ttgcatgggt acaaacgccg cgtcctggtt ttggtcgctt
      781 tgatgcggag gtttcgtcgt gattgtgggc tcacatcgca cgtcgtgcgg cgtcggtttc
      841 gttgcgcttg ccatgagcct taacggctgc gctcatcctc aatccttgcc ggtgcgggtt
      901 ctattgatca gcgaccgaga cacgtgcgcg tgcagccggt tcgcagaccg actcaccggg
      961 cggggcgagg ttcagattca tctcgtggag agcctcgacg gcatctcatc atctgcaaag
     1021 accgatgtct tgctgctgga ttgggcctca ccgatcgagc tcgatgacgt caccgagcgg
     1081 aacatcgttc agttcgtacg atccggcaaa gggattgtgg ccatgggata ctcgctgaaa
     1141 ggcgtttgct catgtccagc actcagcggc atacttggcg gttcggccaa gagcacgatg
     1201 cggtcaggcg atgtttcctt tgtcgttctc gaccccgctg atgcgacgat gttgggtatc
     1261 ggcgggggcc tggcttgggt ggatgagctc cccatcatca atccgtcgat cacgcgggat
     1321 aacaacgttc tgatccgaac ggccgagccg ccggcctttc caggcgacgg gattgcaggt
     1381 aaacctgtac ctgtcgcatg gaccagacga gtcggcgaag ggcgcgtgtt cgcgaccgtg
     1441 ctggggtgcg aagatgccgt gctgggggac gagcggttca ttactatcat tcacaatgcc
     1501 ctgcgatggt gtggcgggcg gatggcggac acaaggcaca atgtgctgag cgtgccggag
     1561 caacaggcag gcttcgagct tctgttcaac ggccgggatc tgaccggttg gaccggcgat
     1621 cctaaactgt ggtcggtcga aaacggcgag atcgtcggac gcggcgagaa gctgccgcac
     1681 aacgtcttcc tcattcacga caaaacctac tctgatttcg tgctgcgtta ttcggtgaga
     1741 ttggtgaatc acaacagcgg cgtgcagttt cgcagtcaac gcttcccgga gttcgtggtg
     1801 aagggttacc aggcggacat cgccgaccgg tggtacggca gcctgtacga ggaaggactg
     1861 gggcggggcg tcctggcgga cggttttgcc ggtaaaggag aaaaggtcgc ccggattgac
     1921 ggctggaacg acatggcggt caaggccgtg ggaacccgca ttacgatcaa gctgaacggc
     1981 ctgaccacgg ctgacttcac ggagactgat ccgaatcggc ccgggagcgg tgtcatcgcc
     2041 ctgcaactac acgcagggcc gccgatggag gtccgcttca gggatatccg cattcggccg
     2101 ctggcggagg ccgcgaaaga gcaggattct ctcaagccct tccggccagg cattgccgaa
     2161 cgcgcttcag gctcttttcc ttgcccagca gtgccagcgt ttcgccgatc ggcgggctga
     2221 tggtcgagcc cgagacggcc acacggatcg gttgggcgac ttttcccatg ttgaccgact
     2281 gctcctgaca aatctgcttg agcagagact ccacgttctc cgcggtccag gggtcaaccg
     2341 actcaagtct gggcaccagc gactcaagca tgcgataccc ggcgttatcc cccttggtca
     2401 acactttctt gatcgcgtcg ggatcatacg cgatgttctc atcggcatca tagatgaagc
     2461 cgcatttgta ctcgatatcg gcgaaggtgc ggaagccttt gttgattctc agcagggaac
     2521 gtctagtcgc ctcgtcgagc ttcgcccggc tgatgcgcaa gtcgggattg acggccaggt
     2581 aatcgtcaaa tgccactgcg agcctttctt cgcttgccga tgccccggcg tcggtgttaa
     2641 aggcgatcag cttgtcgcgg tcgaacctgg cgttggttcg tccgatgcga tcgatgctga
     2701 agagttcgat catttcttgc agcgtcatct tttcgcggtc ccggcccggt gaccagccaa
     2761 gcagggcgat gaagttgacc aggacctccg gcagatagcc ggcctgtcga aaagcgaaaa
     2821 cctccacagc gccgtcgcgc tttgacaact tccgtccctg catgtccatg atgagcggca
     2881 ggtgtgcgta acggggcgtc gtaaagccca aggcccgctg gagagcgata tggcgcggcg
     2941 tctgggacag gaactcctgc ccgcgaagca cgaagttgac ccccatagcg gcatcatcgg
     3001 cgacattggc caggtggtac gtgggcatgc cgtcagcctt gaggatgata aagtcgtcca
     3061 gttcacgcgc tgagacggtg acatctccca tcacttcgtc gtggacggtg acgtcctggc
     3121 cgggggcctt gaatcgaacg accaccggtc gaccggtctg ccgtgccttg cggacgtcgc
     3181 tttcgtccgg aaaggtcgcc gggcgggggt acatgaacgc ttgcttcttg gcctcggctt
     3241 cggctcgcat ggcctccaac tcctcggcgg tgtcaaacgc gtagtaggcg ttgccgcttt
     3301 cgaggaggcg attcacgacg gagcgatggt actccagtcg ctgtgattgg taatagggcc
     3361 cgtttggtcc gcccacgccc acgccttcgt cccactgtaa gccaagccag cgcaggtctt
     3421 ccatgatctt ctgatccgcc ccatcgacgt ttcgagcggt gtcggtgtcc tcaatgcgca
     3481 ggatgaacgt tccgccggtc tggcgggcga tgagccagtt gaacagagcc gtgcgggcac
     3541 ctccgatgtg cagatatccg gtgggtgatg gcgcaaagcg cacgcgggcg ggtgatgttg
     3601 aactcataac gctttcttgt ctgcctcggt gtcagtggat ggcggaacaa ggccggtcgc
     3661 cggtgcggcc gcggcccgac cagcccggcc ggtcaggcgg ccggcgacgt agcgagccgg
     3721 cccgctcaga gcaaaagccc atgcgatcac cagcaggctc aactcggtgt agatccagat
     3781 gagcgggaac gccagcagaa tcaaaatcac gtgttcgaga ggccggcggc gcaagagcca
     3841 gttggcggcg tgggcatacc gcaaccggct gaccatcaag agcgccaaca ccagtgtaca
     3901 gaacggcaaa accttggtca ggaaattggc cgactgcgcc cattcgctgg atgcatcgat
     3961 gctctcgtgc aggaaaacga cgctgattac cgcgatggcc gcaccgggtg acggtagacc
     4021 cttgaaaccc tggtgagccg cttcgtccag gcttgcctcg acgttgaatc ttgccaggcg
     4081 aagagccgcg caacacgcat agatgatccc cacgaacagg gtcaaccggc cgaagtgttg
     4141 gaactgcagg ggtgcatagc cccattgggt gatttcgcgg tgcaccaacg tgaccatcat
     4201 gaccgccggg gcaacgccga agcttacgat gtcggccaga gagtcaagct gctctccgaa
     4261 cttgctggct tgtcctgttt tgcgagccac tcgaccatca agcgcatcgc acaataacga
     4321 ccctacaatc atccatgatg cgaatgagag gaacgaaggg gcgtatttct gcaagagctc
     4381 gcgcctgaac gtcagggcca catcggggct tctcggggca tggatctctt caaggtctcg
     4441 tccacaactg tgtattgccg cgaagccgaa gcagaggttg ccgagcgtca gcagcgttgg
     4501 cagcatgcca atggtgcgca gtcgtcggcg tttgcgggcg gcgcgtctct cgatggcggc
     4561 actctcgcgg gtaacgggtt tgagcatcgg tcatgactcc ttggcaattg accgcgagtt
     4621 cgcttggcgg gccacaacgg tcacggctcc ataagcgcgg tcgccaacgt ttacggccgg
     4681 ggcataacca ctgtcggtcc tgatgatcag ttccgtccgc gagccgaact tgatcaggcc
     4741 gatccgctgg cccgtttcga cgcgatcgcc gaccttgacg gcgcacacca cgcgccgagc
     4801 gatcaatccg gcgatctgtc ggaccacgac cggccctttg gacgggtcgt ccggctcgat
     4861 gacgatggtg ttggcctcgt tgagccggcc ggaatccggg tcccgggcgt cgaggaactg
     4921 gcccggctgg taggtgattc gccggaccgt accggaacaa gggctgcgat tcacgtggac
     4981 gtcaaacacg ctcaaaaaga tgccgattcg cgttgccggc gagcccacgt cagggtgccc
     5041 ctctaggttc gatacttcga ccaccttgcc gtcggcaggg gcgaccagaa tccccgtcac
     5101 agtcggggtc tgccgttgcg gatcccgaaa gaaagccatg ccacccagcc aaagaacggt
     5161 cgctaacccg cccgcgaacc atgcccagga gtgccccgag gcggcaacga catagaaaga
     5221 cgcggccggg caggccaaca ccagtgtcag gatgatcatc tcccggcaac cggccggggc
     5281 caatgggatt cgcatctcac gtgactcccc caaggcttct ccacccccgc tttatcgtag
     5341 cagcaacggt tcgcctgcaa aaggtcggcc gagaggctgc accccgcccg gccatgaccc
     5401 tgcccgcaac cgcattctcg gaccttcgcg ccccgcaagc ggtcgttccg caacttcgtg
     5461 ggacatgagt cgtagcagaa gatgggtgca ttgacctgcc gcaggcttgg gggcatgatg
     5521 caattctgca aacccggctt tgccgggaac tctcggaccg cgcgagcgtc taatatggcg
     5581 aaacagacac cggtatcggc ccagcccggc accatcaatg tcgaggacgc gctgctggtc
     5641 gaacaggtcc gagcgggcga catgagtgcg ttttcccgtc tggttgcccg atatcaggac
     5701 cgtatcgtca atacctgctg gcgagtcagc gggaaccacg acgacgccca ggacctggct
     5761 caggaggcgt tcctgcgggc cctggagcgc atcgactcgt tcgagcagag ggcgggtttc
     5821 tatacctggc tgtttcggat cgccgtgaac ctggccctgt cgcatcgacg taaggcagcc
     5881 caagtggtga agctgtcgct gcacggctcg gacggcggca tgcggacgga gcatcaggcg
     5941 gcgcggctgg ttggccgggt cacgcgggag gctgacgacc cctccgcgaa ggtggcatct
     6001 cgtgaggccg gcaggctcgt ggccgaggcg atcgagcaac tggacgacga gtcgcgagcg
     6061 gtggtagtgc ttcgcgatat cgagggattc gattatcagc agatcgccga gatgctggat
     6121 ctgccgatgg ggacggttaa gtctcggttg taccgggcaa gaatggagtt gcgcgggcgg
     6181 ttgggcccgg agttgtcgcc tggatgaggt ggtgccgccg aaggagcggc caatgtggta
     6241 tgaacgctga acgacgcgag tatctggaga tgcaactgtc cgcctacttg gacggtgaat
     6301 tgacaccgca ggagcaggcg gaagttgagg cgtggctggc cgccgatccg gaggcccgcc
     6361 ggcttcttgc gcagctccgt gcaaccgtcg aagctgttca gtcgctgccc cgagcccggg
     6421 cgtcggagga actgctgggc aatctgcgga ccagactgga acgtaaagcc ctgctggacg
     6481 cgccgggtcc ggccgaggta gtcaccagaa cgccgacccc gtcgtggggc aagtgggttg
     6541 cggcggcggc cgtgatcgcc ctaaccgtca cggccggtta cttcacctgg acgctacgat
     6601 cggagcaaca ggtaggtggc acacaactcg catctcgcga ccgcaaagag gctgctgagc
     6661 cagagttttc ggcggcgacg gagaggacaa agcaagacat tgcacccgag gagttgttca
     6721 agcaccgcga ggccggggac acgactttag ccgccgggcc ggccgtttca cctccggctt
     6781 ccgcgagtcc cgcacgtaaa ccatcggaac gggcgcccgg aggagcacct gccttgccgg
     6841 ccccaggcaa acctctcgca agtctgaacg agggagaatc ggtccgcgca gagcaaggca
     6901 aaaagggtga ttcattagcc gaggccccgg ccgctcgtgg agccgtggct gatgcactga
     6961 tcggcggtgc agagaaagat gtactcgggg acaaaaagcc ggatatgttg agctttgccg
     7021 gtcggcctga ggggcgcagc gcagaaagcg gaggcata
//
LOCUS       DAOTPW010000279         7005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9350_length_7005_cov_5.656547, whole genome shotgun sequence.
ACCESSION   DAOTPW010000279 DAOTPW010000000
VERSION     DAOTPW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9350_length_7005_cov_5.656547"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="P5316_18925"
     CDS             complement(<1..965)
                     /locus_tag="P5316_18925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87089.1"
                     /translation="MKRTVLAGGAGVLAAPYIVPASVLGRNGAIPPSEKIVLGGLGIG
                     GRGTHIVKWMLPEKDVQFVAVCDAQKSRREAVKKLVDAHYGNNDCAVYADMREFLATR
                     TDIDALIIATGDRWHAPATVMAMRAGKDVYSEKPSCMTIAEGQAVVDAAKKHGRIYQT
                     GTQRLSEANFVFCIEMARSGRLGEVHTARAHIAPWDAAELTHDWLPPEPLPPKEEIDW
                     DLWLGPCPWRPFNSTYTRGGWRGHYDFHTSCIGEWGAHTFAQAQAGLNLLDTSPVEYQ
                     YVKNSTGDGMVTRFANGVKMILHREGWWKGSCGMRFEGSEGWVSAA"
     gene            1322..1552
                     /locus_tag="P5316_18930"
                     /pseudo
     CDS             1322..1552
                     /locus_tag="P5316_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294242.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
     gene            complement(1662..3344)
                     /locus_tag="P5316_18935"
     CDS             complement(1662..3344)
                     /locus_tag="P5316_18935"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRR87090.1"
                     /translation="MRRLVIILLVVASVVAVVQAADVRRLPVKEYRDKMKAGWLGQMA
                     GVAWGAPTEFKWKGVIIPADKMPKWVPETINGSFQQDDLYVDMTFLRTLEVHGLDVSI
                     RQAGIDFANSGYKLWHANKAGRDALRQGIAPPDSGHPQFNKHADDIDYQIEADFAGLV
                     SPGLPNTVIALGEKFGRLMNYGDGLYGGQFVGGMYAEAFFEKDPIKLVEAGLRCIPQG
                     SQYAECVRDVLAWYREKPDDWEATWHRINEKYHKNPAYRRASCDKGDFNIDAKINGAY
                     IVVGLLYGKGDLDQSIIISTRCGQDSDCNPSSAAGVLFTTLGFSRIPGRFKEKLDEEQ
                     YWSHTSYNFPKLIDACEKVARQAVLKAGGKIEKNAGGEEVFVLPIAEPVPSKLEQCWE
                     PGPIAGGRFTPEEMAQIKPPAEKPRESARVDLSEALAKFAPGWMARDCGADMSPGIRD
                     ELGKKNVLVTHPLSESMPCVLGRRVDIPPGKKTTLHLVVGHHPQGDWVLRVSADRQLV
                     HKVIGKETAPDGWTSIDVDLSEYAGQIITLQLTNEANGWSYEAAYWAKIEIE"
     gene            complement(3445..4875)
                     /locus_tag="P5316_18940"
     CDS             complement(3445..4875)
                     /locus_tag="P5316_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87091.1"
                     /translation="MSNPLTRRELLGTAAVGGALLASRLSAGAEAASQRPPWPQLPPA
                     KIHKVFAGRTGDAYLTRPTEELAKFDKYFAEIEARLGDVKFIGGDMVPPAEVDQVAGK
                     LKDADALLIVHLSGHGGDAPVLSKLIDVGLPTALFSQPFSGHGWMYFPQWHKQGKRVV
                     LLPTSDWGEIEKVAGLLRAAAWMKRTRILAVGWPHGTPAACSAEEVKKRLGAELVTIP
                     NERIMEAMKAVDAKAAEAEAQEYWIGPAREIVEPTRPEIVESARMYLAVKDIMVQEEA
                     QAICSVHCMGDPRGCLTFSKLNDLGLVGACEGDIDSTLTMLLFAYAFRVPGFITDPVI
                     DTAKNAMIHFHCTSATKMDGPGGKRLPFRIRTQTDSRGGVALEVENRVGQPVTCAKLV
                     NLDAMLVCTGKIIETSRSPLACRTQFAQSVPDARRLFLNWGADVIKGDVMTLLHRVVF
                     YGDHMKAVQDLGDLMGFRVLEEGGVA"
     gene            complement(5036..5911)
                     /locus_tag="P5316_18945"
     CDS             complement(5036..5911)
                     /locus_tag="P5316_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRR87092.1"
                     /translation="MQADDRVSRRAVLGQVAAGTAGAMMAADLAKAEDAVAAPAKGRI
                     RQSVSKWCYGKMTMDELARHCVRIGYQSIELVGPEDWPTAKKYGLTCAMVPGAGKLTD
                     CLNRRENHDRCEAEFRKNIELAAETGMPNVICFSGNRNGISDEEGIRNCVDGVKRVVG
                     FAEKMKVNICMEYLNSKIDHKDYQFDNMAFGVAVAKQVGSPRFGILYDIYHAQIMEGD
                     IIRTIRENHQYILHYHTGGNPGRHEIDETQEIYYPAVMRAIVETGYKGFVGQEFVPAG
                     DPVKALENAFRICDV"
     gene            6133..6552
                     /locus_tag="P5316_18950"
     CDS             6133..6552
                     /locus_tag="P5316_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87093.1"
                     /translation="MDPLKDPVIIAELSRAWRESASNDPQNRHEEGGYIVQNSNLSYG
                     VQRWPRGDQSRIQPPPLEANNCYNGKSVVATFHTHPNPPVDEHGREWEQGPSESECRW
                     HRRRRLRGYVISRMLVYEIGVEASVSVAGKRDEVLLP"
     gene            6549..6764
                     /locus_tag="P5316_18955"
     CDS             6549..6764
                     /locus_tag="P5316_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87094.1"
                     /translation="MILLLNPLLEGLEAARRAAPDAINQDDEVTVDDQGDHWIFEFIP
                     RGDMLGGGARVWIAKEGFQVLRVVRGQ"
BASE COUNT         1257 a         2244 c         2081 g         1423 t
ORIGIN      
        1 gctgcagaga cccagccctc ggagccctcg aaccgcatgc cgcacgaccc tttccaccag
       61 ccctcgcggt gcagaatcat cttcacgccg ttggcaaacc tggtgaccat gccgtcgccg
      121 gtggagttct tcacatattg atattccacc ggcgaggtgt cgagcaggtt aagaccggcc
      181 tgggcttggg caaacgtatg ggcgccccac tcgccgatgc aactggtgtg gaagtcataa
      241 tgacctcgcc agccgccgcg tgtgtacgtc gagttgaacg gccgccatgg gcacgggcca
      301 agccataaat cccagtcgat ttcctccttg ggcggcagcg gctccggcgg cagccagtcg
      361 tgcgtcaatt cagccgcgtc ccaaggggcg atgtgcgccc gggcggtatg aacctctccc
      421 agtcggccgc tgcgggccat ctcgatgcag aacacgaagt tggcttcgct gagccgctgc
      481 gtgccggttt gatagatgcg cccgtgcttc ttcgcggcgt ccaccaccgc ctgtccttcc
      541 gcaatagtca tgcacgaggg tttctcgctg taaacgtcct tgccggcacg catggccatc
      601 accgtggccg gcgcgtgcca tcgatcaccc gtggcgatga tcaaagcgtc aatgtcggtg
      661 cgcgtggcta ggaattcccg catgtccgcg tagacggcac aatcgttgtt tccgtagtga
      721 gcgtccacga gcttcttgac cgcttcgcgc cgcgacttct gagcgtcgca cacggccacg
      781 aactgtacat ccttctccgg cagcatccac ttgacgatgt gcgttccccg tccgccgatg
      841 cccaggccgc cgagcacgat cttctcgctc ggtggaatcg caccattccg gcccagcacc
      901 gacgccggca cgatatacgg cgccgccagc acacccgcgc cgccggcaag aaccgtgcgc
      961 ttcaacacct gtcgccggct aagcgcggct tgagcggatc cggaagagaa cttgcgtgat
     1021 tgccccagct tctgatcgct gccgttctga gttgaatgtt gtgaggacat atggacgatt
     1081 atacgtcccc gccaacaaac cgcaagcatg ttagtcaaaa ctcctaccgg ccggaggaga
     1141 ggggagtggg tacgagttgg tgtcagccaa agcggcctta ccacgagtga tcgcggcctc
     1201 acagccgccg cattgcacat gaatcccgct atcaggtttc ccgtccgcac gggtttgacg
     1261 acgatcttcc aggctggatt tcgatgtgtc gatctgctag agaggggtgg ggggggttgc
     1321 tatggccaca ctgacactgg aaatgcctga agacgttttt gccgccatgc gaagctcacc
     1381 cgaggagttc gcccgcgaga tgcgtctggc agcagcgatc ttctggtacg cccgcggaga
     1441 tgtctctcag gaagaaggag cccgcattgc cggtctggat cggaccgatt tcatcttggc
     1501 tctggcccgg gagaaggttg acgtgatcca tgtcgatgtg gaggagctga agaacgtaat
     1561 acacggacca caagagggcg ctcaggctga atctcatgtc ggcgaccctt cacaggccgt
     1621 tcatggccat cgaaggcgag agtcgccgca cgagggattt cctactcgat ctcgatcttc
     1681 gcccagtaag ccgcctcgta ggaccatccg ttcgcctcgt tggtcagttg cagtgtgatg
     1741 atctggccgg cgtactcgga caggtccacg tcgatacttg tccagccgtc gggcgcggtc
     1801 tccttgccga tcactttgtg aaccagttgg cggtcggccg agactctcag aacccaatcc
     1861 ccctggggat ggtgccccac caccaggtgc agcgtggtct tcttgccggg cggaatgtcc
     1921 accctccgcc ccagaacgca aggcatgctt tcgctcagcg gatgagtcac cagaacgttc
     1981 ttcttcccta gctcgtcgcg aattcccggc gacatgtccg cgccgcagtc cctggccatc
     2041 cagccggggg cgaacttcgc gagcgcctcg ctgaggtcca cccgcgcgct ctcgcgaggc
     2101 ttctccgccg gcggcttgat ctgagccatc tcctccggcg tgaacctgcc gcccgctatg
     2161 ggcccgggtt cccagcactg ctccagcttg ctgggcacgg gctcggcgat cggcaggacg
     2221 aatacctcct cgccgccggc gttcttctcg atcttgccgc cggccttcag cacggcctgc
     2281 cgggcgacct tctcgcaggc gtcgatcaac ttcgggaagt tgtagctcgt gtggctccag
     2341 tactgctcct cgtcgagttt ctctttgaag cggccaggga ttctcgaaaa cccaagggtc
     2401 gtgaaaagca ccccggcggc gctcgacggg ttgcagtctg agtcctgccc gcaccgcgtg
     2461 gagatgataa tcgactgatc caggtcgcct ttgccgtaga gcagccccac gacgatgtag
     2521 gccccgttga tcttggcgtc gatgttgaag tctcccttgt cgcaggaagc cctgcggtag
     2581 gccgggttct tgtggtactt ctcgttgatc cggtgccagg tcgcttccca gtcatccggc
     2641 ttttctctgt accaggcgag cacgtcgcgg acgcattcgg cgtattggct gccctgcgga
     2701 atgcagcgca gaccggcctc gacaagcttg atcggatcct tctcgaagaa cgcctcagcg
     2761 tacattccgc cgacgaactg cccgccatac agcccgtcgc cgtaattcat gagccgtccg
     2821 aacttctcgc ccagcgcgat gacggtattg ggcaggccgg gcgagaccag gcccgcaaaa
     2881 tcggcctcga tctgatagtc gatgtcgtcg gcgtgcttgt tgaactgcgg atgaccggag
     2941 tcgggcgggg caatgccttg gcgcagagcg tcgcgtccgg ccttgttcgc atgccagagt
     3001 ttataaccgc tgttggcaaa atcgatgccc gcctgccgga tcgacacgtc gagcccatga
     3061 acctccagcg tgcgcagaaa ggtcatatcg acgtagaggt catcctgctg gaagctgccg
     3121 ttgatcgttt ccggcaccca cttgggcatc ttgtcggcgg ggatgataac acctttccat
     3181 ttgaactcgg tcggcgcccc ccatgcgacg ccggccatct ggccgagcca gccggccttc
     3241 atcttgtcgc ggtattcctt gaccggcagt cggcggacgt ccgcggcctg caccacagca
     3301 acaacggatg cgacgaccag cagaatgatg accaggcgtc tcatggagtc ctcctcgtaa
     3361 ctgattctca ggtccaagtg tatcgcggtc cttggcccgc tgcggaccgc cttgcagccg
     3421 ctgaccaagg ctccacccgt tttgtcaggc gaccccgccc tcctcaagca ctctgaatcc
     3481 catcaggtcg cccagatcct gcaccgcctt catgtggtcg ccgtagaata ccacgcgatg
     3541 cagcagggtc atcacgtcgc ctttgatgac atcagcaccc cagttgagga aaagcctcct
     3601 ggcatctggc acgctttggg cgaactgagt acgacaggcc agcgggctcc tgctcgtctc
     3661 gatgatcttc cccgtgcaga ccagcatcgc gtccagattg accagcttgg cacaagtcac
     3721 cggctggccg actcggttct cgacctccaa agccacgccg ccgcggctgt ccgtctgggt
     3781 tcggatcctg aacggcagcc gtttgccgcc cggcccatcc atcttcgtcg cggacgtgca
     3841 gtggaagtga atcatggcgt tcttggccgt gtcgatcacc gggtcggtga tgaagcccgg
     3901 cacacgaaat gcgtaggcga acagcaacat cgtgagcgtc gagtcgatgt caccctcgca
     3961 cgcccccacc agccccaggt cgttgagctt gctgaaggtc aggcaacccc tcggatcacc
     4021 catgcagtgt acgctgcaga tcgcctgggc ttcctcctgt accatgatgt ccttgaccgc
     4081 caggtacatc cgcgccgatt cgacaatctc cggtcgggtg ggctcgacga tctcgcgcgc
     4141 aggtccgatc cagtattcct gcgcttcggc ttcggctgct ttggcatcga cggccttcat
     4201 cgcttccatg atccgctcgt tgggaatcgt caccagttcc gcgcccagcc gcttcttgac
     4261 ctcctcggcc gagcacgcgg cgggcgtccc gtggggccaa ccgaccgcca ggatccgcgt
     4321 ccgcttcatc cacgccgccg cgcgcagcag cccggcaacc ttctcgatct ccccccagtc
     4381 gctggtcggc aggagcacca ctctcttgcc ctgcttgtgc cactgcggga agtacatcca
     4441 gccgtgcccg ctgaaaggtt gtgagaacag cgccgtcggc aggcccacgt ctatcagctt
     4501 gctgagcacc ggggcgtcgc cgccgtgtcc gctgagatga acgatcagca gggcgtcggc
     4561 gtctttcagc ttgcccgcca cttggtccac ctctgccggc ggtaccatgt cgccgccgat
     4621 gaacttcaca tcgccgagac gggcctcgat ctctgcgaag tacttgtcaa acttggccag
     4681 ttcctccgtg ggtcgagtca gataggcatc accggtgcgg ccggcaaaaa ccttgtggat
     4741 ttttgcaggc ggcagttggg gccatggcgg cctctgggat gcggcctctg cacctgcgga
     4801 caaccgcgac gccaacaacg cccctcccac cgcggcggtt cccagcaact ctcgtcgggt
     4861 caaaggattg ctcatgcctt gttcctccgg aagctgatcc aacttgccca atgagactgt
     4921 ctcaccaaac acggcgagta cggtgcctca ttatcaggcg gtgcattccg aaaagccaga
     4981 tgtattccac gagcggctac gccacgggtc cggtggccat gcaaacgcgc tgagatcata
     5041 cgtcgcagat tcggaaagcg ttttccagcg ccttgaccgg atcgccggcg ggcacgaact
     5101 cctgacctac aaatccctta taacccgttt ccacaatcgc ccgcatgacg gccgggtagt
     5161 agatctcctg ggtttcgtcg atttcatgtc gcccggggtt gccgccggtg tgatagtgca
     5221 ggatgtattg atggttctct ctgatcgtcc ggatgatgtc gccttccatg atctgggcgt
     5281 gatagatgtc gtagagaatg ccgaatcggg gcgagccgac ctgcttggcg acggccacgc
     5341 cgaaggccat gttgtcgaac tggtaatcct tgtggtcgat cttgctgttg agatactcca
     5401 tgcagatgtt gaccttcatc ttctcggcga agccgacgac tcgcttgacc ccgtcgacgc
     5461 agttgcggat gccctcctcg tcggagatgc cgttgcggtt gcccgagaag cagatgacgt
     5521 tgggcatgcc ggtctcggcg gccagctcga tgttcttgcg gaactcggcc tcgcagcggt
     5581 cgtggttctc gcggcggttg aggcagtcag tcagcttgcc ggcgccgggc accatcgcgc
     5641 aggtcaggcc gtacttcttg gccgtcggcc aatcctccgg gccgaccagc tcgatcgact
     5701 gatagccgat ccggacgcaa tgacgggcca actcgtccat cgtcatcttg ccgtagcacc
     5761 acttggacac cgactggcgg atccgcccct tggccggggc cgcaacagca tcctccgctt
     5821 tggccagatc agcagccatc attgccccgg ccgtcccggc ggctacttga cccaagactg
     5881 ctcttcgtga cacgcgatca tcggcttgca ttgtctgttt cctcatgtgt ccctgtcagc
     5941 acctcacgac ggccattcta gagccgatct cgcagagaat cacgaaaagc aggactgaag
     6001 aggtccatcg caaccatctt gttcgtagga ggttacagaa cgaagctatt cttggccacc
     6061 gatcgagagt gagttgcgag gatttgcaga cagggtttat cgggggtaca ttggccacag
     6121 tgggagggtg ccgtggatcc gctcaaagac cccgtaatca tcgccgagct cagccgtgca
     6181 tggcgagagt ctgcgtctaa tgatccgcag aaccgccatg aggaaggggg gtatatcgtg
     6241 cagaactcaa atctctcata cggtgtgcag cgatggccgc ggggtgacca gtcccgcatc
     6301 cagccaccac cgttagaggc gaacaattgc tataatggaa agagcgtggt tgccactttt
     6361 cacacgcacc cgaacccgcc cgtggacgag catgggcgag aatgggagca aggcccgagc
     6421 gaatccgagt gccgctggca ccggcgtcgg aggctcaggg ggtatgtgat cagccggatg
     6481 ctcgtgtacg agattggcgt agaggcgagc gtgtcggtgg ccggaaaacg cgacgaggta
     6541 ctcttgccat gatcctgctt ctgaatccac ttctcgaagg actcgaggcc gctcgacgcg
     6601 cagccccgga cgcgatcaat caggacgacg aagtgacagt ggacgatcaa ggcgatcact
     6661 ggatcttcga gttcatccca cgcggagaca tgctcggcgg cggcgcgcgt gtttggatcg
     6721 ccaaggaggg ttttcaggtc ctgagggtcg ttcgggggca gtgagcctcc tggttgctct
     6781 gacggatcgg aagcggccgt cgagcgagat atggcttctt gccgagtgcc ccatcccggg
     6841 tgggggacgg catcggacgc cggagtgcct gcctgagcat cagcccacct ttgcggtctt
     6901 cgcagctctg cgtgagactt gaacgaggga tgaaccgcca tgacgccatg acgccaagaa
     6961 gacaagctgt ccgcgctcaa cctttccgcg tcatcggcct gattc
//
LOCUS       DAOTPW010000280         6999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9356_length_6999_cov_5.859879, whole genome shotgun sequence.
ACCESSION   DAOTPW010000280 DAOTPW010000000
VERSION     DAOTPW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9356_length_6999_cov_5.859879"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="P5316_18960"
     CDS             <1..318
                     /locus_tag="P5316_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87095.1"
                     /translation="SWDSPGAGGEMGRIRGQRGTFYGKYEGLEKNLPDTRRPPLPPKV
                     EAGGHGGSHGHLMNEFVTAILQDRKPLVDIAQALNMTVAGIVAHQSALKDGELLKIPQ
                     YKL"
     gene            331..1710
                     /locus_tag="P5316_18965"
     CDS             331..1710
                     /locus_tag="P5316_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HRR87096.1"
                     /translation="MNAGRESGSLLYVVLLVLVAALGGLLFGYDTAVISGATEFLSQK
                     FALDENWEGWAAACTLLGCMLGAGTAGTLSDRFGRKKTMLLAAVLFGLSALGAALPRN
                     LNQFVMARMIGGLGIGIASMMSPMYIAEVSPSRIRGRLVSVNQFAIIFGMLVVYFVNA
                     YIARQGGADDQDWNIHYGWRWMFGSGVLPAVLFFVLLFFVPESPRWLVSRGQEDRAES
                     VLARIGGKAHAGREIGEIRDALSLETSSLGQLFAPRMRLPLLIGIGLAVLQQITGINI
                     VLYYAPKIFSSTGLKITDAIGHTVLVGAVNLAFTVVAIWVVDRVGRKPLLLTASAGMG
                     LSLLLLGEAFRRERFHGPWVLVLVLAYVASFAIAMGPVVWVVIAEIFPTGIRGRAMAV
                     ATVCLWFACFMVTQTFPRMLKHLGGNTFFIYAVMCAFCFVFVAAFVPETKGKSLEEIE
                     RGWMRRENR"
     gene            complement(1740..3182)
                     /locus_tag="P5316_18970"
     CDS             complement(1740..3182)
                     /locus_tag="P5316_18970"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRR87097.1"
                     /translation="MRQSAAAVTGVVFAALLALAGLAASAVLLQRHVAVEVGSDPVLG
                     AMCEDAEGQSSCDAILASKWGKIVLGRGDKSVTIPTAMFGFAFFACMASWYITVGQAR
                     GSRRWLHFLPVLGAAVGAVICIWLDVIMWSRFDRPCWLCFLVHVLTLALLLVTLLLWP
                     RQGMAAGPAVAPAGHIVESSYGRQSELSHPPLHLVLAAVLTAAAMSAAGWGIYQSRLR
                     KAYSNEYFAKWQEYDGDYAAKHAKFMSQPQLQIPIMPEDPVRGPADAPHTVVVFTDFQ
                     CPHCRLIADMLDERMKEYPGRFRVVFKYFPMNTTCNIYIKNTPHGAACAAAVTAEAAR
                     ILGGNETFWKMHDELFRDPEGFAKRSTEYVKQACEKLGIDHDALWKQIKTYGIWDRIR
                     SNVGQGSALGVNATPTLFFDGRRMAGWGDRHTWRFLVESPPSKTPHAVPATYPVLGTR
                     PASTAPSVPVTTTRAAAAPPTTRAATAPAM"
     gene            complement(3219..4841)
                     /locus_tag="P5316_18975"
     CDS             complement(3219..4841)
                     /locus_tag="P5316_18975"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRR87098.1"
                     /translation="MRTGPIRTVVWLAAMASCVAGIWLSRELTFEYLKTHGFAPVAAA
                     TENDGETSWFDAICSATESASCEEVAQSPYSVWPFGSKPGQFHVPVALLGMFYFTAVL
                     AWLGLVGICSPSRWWAHLILVSGTAVGLGVSAFFEYVMWTRLKYYCPLCVITHVLSLL
                     LFVFALALWPRESDEAAAAPPRASATPAVAWFAPSVTHWPTLRILVATPIVALVAIWG
                     EYQWLSPTVAAERSVVEKEVSTTQPVDEKELLATLTTQPAEVLIQKVIDAHKKLTTAE
                     AYQKHYQKQLDYYQTRWQHAYTAWEISPPVPISLEDRPFRGPVNAPHTLVVYSDFGCP
                     GCKRFEDYTNTRLLPLSERYGGLIRMTFKYWPICTDCSPYASHNLHPAACKAALAAEA
                     ARIVGGDEAYWKMHDLLWDSQARWKGWKENRDFNVLAREIGLDEQAFEQAMAGDQAMA
                     RVKADLDEGANLGKNLPNIKPGDVDFIKVDSTPAIFVDNKRVWRPHATDALWLKIMTS
                     RVPSSSIPVSGTSAAPTRQDERPSMAATRPQP"
     gene            4974..5738
                     /gene="purQ"
                     /locus_tag="P5316_18980"
     CDS             4974..5738
                     /gene="purQ"
                     /locus_tag="P5316_18980"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HRR87099.1"
                     /translation="MILRTAGTNCDEETVHAWELAGAQARRVHVRELIDKPALLREFA
                     ILTIPGGFSYGDDISAGKILANQMVHHLADAIGEFVAAGKLVLGICNGFQVLVKAGLL
                     PGDGQSFMSRQTVTLTNNDSARFEDRWVHLRTGKRATAFFPADTILAMPVAHGEGKLV
                     AADDQTLRRLIENGHVAVTYCDAQGRPGPYPVNPNGSQADIAGLTDRTGRIFGLMPHP
                     ERHVHPTQHPEWTRRPGASADGRIIFETAVRTAFQL"
     gene            5804..6103
                     /locus_tag="P5316_18985"
     CDS             5804..6103
                     /locus_tag="P5316_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87100.1"
                     /translation="MTPVAHTRFARRFVPAEAGMGTGPSIEPLFDALDARPIETVGQF
                     EQWILDGSELASCLSEEHDKRLVAMTCQTDDQSPSKSPTAAFGRNLRAVRPTPTC"
     gene            complement(6111..6251)
                     /locus_tag="P5316_18990"
     CDS             complement(6111..6251)
                     /locus_tag="P5316_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87101.1"
                     /translation="MERMTVSRGIVEPLISLELVVSEAVETGIRGNLRTQPDHPVEDL
                     ID"
     gene            6565..>6999
                     /locus_tag="P5316_18995"
     CDS             6565..>6999
                     /locus_tag="P5316_18995"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRR87102.1"
                     /translation="MTSDLLGREWRREDGAHLKVERCLIDANWGTSTDVVYQFCRQSA
                     HAAVLTPSHGRFVGASSIPLAEYRRKRGDRVGLNWRIPSVHGRRAIRHVLFDSNYWKS
                     FIHARLAVAMGDRGCLSLFGRDPEVHRLLAEHLTSEYRVKTEG"
BASE COUNT         1272 a         2204 c         2201 g         1322 t
ORIGIN      
        1 agttgggact ctccgggcgc gggcggcgag atgggccgga ttcgagggca gcggggcacg
       61 ttttacggca agtacgaggg actggagaag aacctgccgg ataccaggcg cccgccgctt
      121 ccgcccaagg tggaagccgg aggtcacggc ggctcgcacg gccacctgat gaacgaattc
      181 gtcaccgcga tcctccaaga ccgcaagccg ctggtggata tcgcccaggc attgaacatg
      241 accgttgccg gcatcgttgc tcaccagtcg gctttgaagg acggtgaact gctcaagata
      301 ccgcagtaca aactgtgacc gagtggtccg atgaacgctg gcagagaatc gggcagtctc
      361 ctctacgtgg tgctgttggt gctggtcgcg gcgctgggcg gtttgttgtt cggttacgac
      421 acggcggtga tttcgggcgc gaccgagttc ctgagtcaga agttcgcgct cgacgagaac
      481 tgggaaggct gggccgcggc ctgcaccttg ctgggctgca tgctcggtgc gggcacggcc
      541 ggaacgctga gcgatcgctt cggtcgcaag aagacgatgc tgttggcggc ggtcctgttt
      601 ggcctgtccg cgttgggcgc ggcattgcct cgcaacctta accagttcgt catggcacgg
      661 atgatcggcg ggcttggcat cggcattgcc tcgatgatgt cgccgatgta catcgccgag
      721 gtttcaccga gccgcatccg cggccgcctg gtttcggtca accagttcgc catcatcttc
      781 ggcatgctgg tggtctactt cgtcaatgcc tacatcgcca ggcagggcgg cgcagacgac
      841 caggattgga acatccatta cggctggcgc tggatgttcg gctccggtgt gttgccggcg
      901 gttttattct tcgtgctgct gttctttgtg ccggaaagcc cgcggtggtt ggtgagccgg
      961 gggcaggagg accgggccga atccgtcctg gctcgcatcg gcgggaaagc tcatgccggg
     1021 cgtgaaatcg gcgaaatccg cgacgccctc tcgctggaga cgagttcact gggtcagctt
     1081 ttcgccccgc ggatgcggct gccgctgctg atcggcatcg ggctggccgt tctccagcaa
     1141 atcacgggga tcaacatcgt gctgtactac gcgcccaaga tcttcagcag caccggcctg
     1201 aagatcaccg acgcgatcgg ccacaccgtg ctggtcgggg cggtcaacct ggcatttacc
     1261 gtcgtcgcca tctgggtggt cgaccgagtc gggcgcaagc cgctgctgct gaccgcctcg
     1321 gccggaatgg ggttgtcgtt gctgctgctg ggcgaagcat tccggcgcga acgattccac
     1381 gggccgtggg tgctggttct ggtgctggct tacgttgcct cattcgcgat tgccatgggg
     1441 cccgtcgtgt gggtggtgat cgccgagatc tttccgaccg gcatccgcgg ccgggcgatg
     1501 gccgtggcca ccgtgtgtct gtggttcgcc tgcttcatgg tcacccagac gtttccgcgc
     1561 atgctcaagc acctcggcgg caacaccttc ttcatctacg cggtcatgtg cgccttctgc
     1621 tttgtctttg tggccgcttt cgtgcccgaa accaaaggca agtcgctcga agagattgag
     1681 cgcggctgga tgcgccgtga gaatcggtaa gcgaccggcg gcagcccaaa ccggccgctt
     1741 tacattgccg gtgcggtcgc ggcccgcgtc gtcgggggcg cggctgcggc gcgggtcgtc
     1801 gttacgggaa ccgatggcgc ggtgcttgcg ggccgtgtcc ccaagaccgg gtatgtcgcg
     1861 ggaaccgcgt gtggggtctt ggagggcggg ctctccacaa ggaatcgcca agtgtgacgg
     1921 tcgccccagc cggccatgcg ccgaccgtcg aagaacagcg tcggggtcgc gttgacgccc
     1981 aaggcactgc cttggcccac gttggagcgg attcgatccc aaatgccgta cgtcttgatc
     2041 tgtttccaga gtgcgtcgtg gtctattccc agcttctcgc aagcttgctt gacatattcg
     2101 gtgctgcgct tggcgaaacc ttccggatcg cggaacagct cgtcgtgcat tttccagaac
     2161 gtttcattcc cgccaagaat ccgggcagcc tcggccgtga cggcggcggc acaggccgcg
     2221 ccgtgcggcg tgttcttgat gtagatgttg cacgtggtgt tcatggggaa gtacttgaag
     2281 accacgcgga agcggcccgg gtattccttc atgcgctcat cgagcatgtc agctatcagt
     2341 ctgcaatgcg gacactggaa gtcagtgaaa acgaccacgg tgtgtggggc gtcggccggg
     2401 ccgcggaccg ggtcctcggg catgatcgga atctgcaact ggggttgaga catgaacttc
     2461 gcgtgtttgg cggcataatc gccgtcgtat tcctgccact tcgcgaagta ctcgttgctg
     2521 taggccttcc gcaaacgtga ctggtagatt ccccagccgg cagcgctcat agcggcggcc
     2581 gtcaatacgg cggccaggac cagatgcagc ggtgggtgag acaactctga ctggcggccg
     2641 tacgaactct ccacgatgtg cccggccggc gcgaccgcgg ggcccgccgc catgccctgt
     2701 cttggccaca gaagcagggt caccagcagc agcgccaggg tcagcacatg caccagaaaa
     2761 cacaaccagc agggccgatc aaacctggac cacatgatca cgtcaagcca gatgcagatc
     2821 acggctccga cggccgcccc cagaacgggc agaaaatgca accaccgccg cgacccgcgc
     2881 gcctgcccca ccgtgatgta ccacgaggcc atgcaggcga aaaacgcaaa tccgaacatc
     2941 gccgtcggga tggtcacact cttgtcgccc cggcccagca ctattttgcc ccacttgctg
     3001 gccaggatcg cgtcgcagct tgactgcccc tcggcgtctt cacacatggc gccgagaacc
     3061 ggatcgctgc cgacctcgac agcaacgtga cgctgaagga gaacggcgct ggccgccaga
     3121 cccgccaagg ccagcagcgc cgcaaagacc acgccggtga ctgctgctgc gctttgtctc
     3181 atatcgtatg tgtcctcgat gttcttgtcc gcgtcggatc acggctgcgg ccttgtcgcc
     3241 gccatggaag gccgctcgtc ctgccgcgtc ggtgctgccg acgtccccga caccggaata
     3301 ctcgacgaag gcacccgcga cgtcatgatc ttcaaccaca gagcgtcggt cgcgtgcggc
     3361 cgccagactc gcttgttatc cacgaaaatc gcgggtgttg aatcaacctt gatgaagtcc
     3421 acgtcgccgg gcttgatatt cggcaggttc ttgccgagat tggccccttc gtccagatca
     3481 gccttgaccc gggccatggc ctggtcgccg gccatcgcct gctcaaaggc ctgttcgtcc
     3541 agtccgatct cgcgcgccag gacattgaag tcccgattct ccttccatcc tttccatctc
     3601 gcctgactgt cccagagaag gtcgtgcatt ttccagtagg cctcgtcgcc gccgacgatc
     3661 cgggccgcct ccgccgccag cgccgccttg caggcggccg gatgcaggtt gtgggacgcg
     3721 taaggactac agtcggtgca gatcggccaa tacttgaatg tcatgcggat caatccgccg
     3781 taacgctcgc tcagcggcaa cagtctcgtg ttggtgtagt cctcgaatcg cttgcacccc
     3841 ggacatccga agtcgctgta caccacgagt gtgtgcggag cattgaccgg gccgcggaac
     3901 ggtcggtcct cgagcgagat gggcacgggc ggtgagattt cccatgccgt gtaggcgtgc
     3961 tgccagcgcg tctggtagta atccagttgc ttctggtaat gcttctggta agcctcggcg
     4021 gtggtgagct tcttgtgcgc gtcgatgacc ttttgaatca gcacctcggc gggctgcgtc
     4081 gtcagcgtcg cgagcagctc cttctcatcg acgggctggg tggtcgaaac ctccttctca
     4141 acaacggacc gctcagccgc gacggtcggc gacaaccact ggtattcccc ccagatcgcc
     4201 accaacgcca cgatcggggt cgccaccaga atgcgcaagg tcggccaatg agtcacggaa
     4261 ggggcaaacc acgcaacggc gggtgtggcc gaggcgcggg gcggggctgc ggcggcttcg
     4321 tccgattccc gcggccagag cgccaaagcg aaaacaaaga gcagcagcga cagcacgtgg
     4381 gtgatcacac acagcggaca gtagtacttc agtctggtcc acatgacgta ctcgaagaat
     4441 gcgctgacac cgaggccgac ggccgtccca cttacaagaa tgaggtgtgc ccaccatcgc
     4501 gaggggctac agattccgac caggcccaac cacgcgagta cggccgtgaa gtagaacatc
     4561 cccagaagcg ccaccggcac gtggaattgc ccgggcttgc tgccaaaggg ccagaccgag
     4621 tagggactct gcgccacctc ttcacaactg gcgctctctg ttgcactaca gatggcgtca
     4681 aaccaacttg tctcaccatc gttttcggta gctgccgcca ccggagcgaa tccgtgagtc
     4741 ttcaggtact cgaaggtcaa ttctctgctc agccagatgc cggcaacgca acttgccatc
     4801 gccgccagcc aaaccaccgt ccggatcggg cctgttctca ccgttgtcac tcctgcggca
     4861 tgggggccga ttcatctcgc ggggggaagc accaactggc gcccgtcggg gctggggttt
     4921 gcaggcccga cgcccgagtc atacactctg gtgccatgag ccaaccaaga gtgatgatcc
     4981 ttcgaaccgc cggtaccaac tgcgacgagg aaaccgtcca tgcctgggaa ctggccggcg
     5041 cccaagcccg gcgggtacac gtacgcgaac tcatcgataa acccgccttg ttgcgggagt
     5101 tcgcgattct aacgattcct ggcggcttca gctacggtga tgacatctcc gccgggaaga
     5161 tcctggctaa ccagatggtt caccaccttg ccgatgccat cggcgaattc gtggccgcag
     5221 gcaaactggt tctggggatc tgcaacggtt ttcaggttct ggtcaaggca gggctgctac
     5281 cgggcgatgg ccagagtttc atgagtcggc agaccgtcac cctgacaaac aacgactcgg
     5341 cccgcttcga ggatcgatgg gtgcacttgc ggacggggaa gcgggcgact gccttttttc
     5401 cagccgacac gattctggcc atgccagttg cccacgggga aggcaagctc gtggccgctg
     5461 acgatcagac tctgaggcga ttgattgaga acggacacgt agccgtcact tactgtgatg
     5521 cccaaggacg cccaggccct tatccggtta atcccaatgg cagccaggcg gacatcgccg
     5581 gtttgaccga cagaacaggc cgcatcttcg gcctgatgcc ccatcctgag cgccacgttc
     5641 accccaccca gcaccctgag tggacccgtc gaccaggagc ttcggcggat ggacgaatca
     5701 tcttcgagac cgccgtccga acagctttcc agctctaacc ttatttctca cagcggttta
     5761 tgaaatacga gcagcgaact gagcctgctc aaggaagctg cgcatgacgc ctgtggccca
     5821 tacccggttc gcccgaagat tcgtgccggc cgaagctggc atgggcactg ggcccagtat
     5881 cgaacccttg tttgacgccc tcgatgcccg gcccatcgag accgtcggcc aattcgagca
     5941 gtggattctc gacggaagcg agctggcctc ttgtctgtcg gaggaacacg acaagcgcct
     6001 cgtcgcgatg acctgccaga cggacgatca gagtcccagc aagagcccga ccgccgcgtt
     6061 cggcaggaac ctccgagccg tccgcccgac gccgacttgc tgatcgggaa tcaatcgatg
     6121 agatcttcca ccggatgatc cggctgcgtt cgcaaattgc cccgaatgcc ggtttcgaca
     6181 gcttccgaga ctaccagttc aaggctgatg agcggttcga ctatacccct gctgactgtc
     6241 atacgttcca cgaagccatc aggcacgctg tggtgccagt ttaccggcag attcagcagc
     6301 aacgcagaag acaccggggg ttgaacgact gcgaccctgg gatctcgccg tggatcccaa
     6361 gggcaggcca cccctgtggc cgttcaatga gggtggcgag ttgtcggcca agtgttggac
     6421 gatcttcgct gaggcggtcc gccttaaagc ctatcccgag accagaagag gcgctacttt
     6481 actctgcggg acgcgacgcg gacgcttcag ctcgccgcca agggtgcggg actggagggt
     6541 gcgatctacg ccgggctgga ggcggtgacc agcgaccttc tcggccggga gtggcggcgg
     6601 gaagacgggg cgcacctgaa ggtcgagcgg tgcctcatcg acgccaactg gggcacatcg
     6661 actgacgtgg tctaccagtt ctgccgtcag agcgcccacg cggcggtcct gacgccgagc
     6721 cacgggcggt tcgtgggggc atcaagcatc ccgctggccg agtaccgccg caagcgcggc
     6781 gaccgcgtcg gcctgaattg gcgcatcccc agtgtccacg gccgccgggc catccgccac
     6841 gtgctcttcg actcgaacta ctggaagtcg ttcatccacg cacgccttgc cgtcgccatg
     6901 ggcgaccggg ggtgcctgtc gcttttcggc cgcgacccgg aagtgcatcg cctcctggcc
     6961 gagcacctga cgagcgagta ccgcgtgaag accgagggc
//
LOCUS       DAOTPW010000281         6928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9499_length_6928_cov_4.748290, whole genome shotgun sequence.
ACCESSION   DAOTPW010000281 DAOTPW010000000
VERSION     DAOTPW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9499_length_6928_cov_4.748290"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="P5316_19000"
     CDS             complement(<1..807)
                     /locus_tag="P5316_19000"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR87103.1"
                     /translation="MGLTSRERVLAAFDHVEPDHVPAWCGSSPEFWAKAKKQLGLDDE
                     ALRIRFGDDFRRVWTRYVGPEVHLQPGAIYATPFGIQRHGMGYGQPMSHPLAEATIEQ
                     IHDYPWPQPDWMDVSAIRDEALRYNRQYAILGGDWSPFWHDAIDLLGMENLMLRMYDE
                     PDAVHTVFRHIVDYYAAVSERIFEAAGDVIDIFFIGNDFGSQTGPLLSEGLFRQFCLP
                     HLRRLVELGHSYGLKVMLHCCGGFAPLIPAMIEIGLDGLHAVQPSCHGMDL"
     gene            complement(798..1121)
                     /locus_tag="P5316_19005"
     CDS             complement(798..1121)
                     /locus_tag="P5316_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HRR87104.1"
                     /translation="MNRYGMVIKVKPEGLEEYKRLHAAVWPDVLKMISACNIRNYSIF
                     HKDGYLFSYFEYVGTDFAADMARMAADPTTQQWWAVCMPCQEPLPTRVNGEWWAKMEE
                     IFHWD"
     gene            complement(1123..2277)
                     /locus_tag="P5316_19010"
     CDS             complement(1123..2277)
                     /locus_tag="P5316_19010"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR87105.1"
                     /translation="MSHAQVKIDRIMAALEHRETDRVPVGEFFWTNFIRRARREGKIG
                     DGFDPYRHWDLDMVVITPNMDPHLTGIQIVRETQEIRTVKTGFGATIEVHASCPMPMY
                     VDFAHTSFEQMEALRFDDPHDERRYFAALDDQINSVGDSLNLNLPPWIDRVKAYADDF
                     CVFGAVCEPHEMLWRIIGTENALYKMAEEPERLARFIERLGDFLVGIAEAEIAAAKGR
                     LTGLYIWGDIAYTRGMFFSPDYWRSVYKPQLKRLCDVARAAGLKTIYHGCGNASVIYE
                     DMIEAGVDGYNPLEAKAGLDVVELKRQYGRRWAFNGNIDVRVLATNDREQIRREVLRK
                     LNAAKGGGYIMQSDHSVPDSVSFDSYDYAVRLAQEYGRYPLQLGECDEAI"
     gene            2524..3540
                     /locus_tag="P5316_19015"
     CDS             2524..3540
                     /locus_tag="P5316_19015"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRR87106.1"
                     /translation="MNMTNKVTWTVLVGVSVMASVPVKLAQTTQEDKTCLQASAEDLQ
                     WCREARFGLFVCWGPVTLKGTEIGWSRGGERRGAGENKNGIPVEVYDNLYRQWKPTKF
                     DARLWCQAAKDAGMKYLIFLVKHHDGYCLYDTKLTDYRSTSPEAAWQHDVMADLTKAC
                     RESGLKLFIYDSQPDWHHPDYHTAHHHRYIEYLHGQIRELLTNYGRIHGLWFDGLGGK
                     ADDWDAPTLFKLVRVFLPHIIISNRCRLPGDFDTPEQKIGKFQTHRPWESCINLGMQW
                     SWKPNNPIKSFKECIGLLVRCAGGDGNLALNVSPTPDGDFEPQQLDQLKEIGGWLARY
                     GESI"
     gene            3886..4674
                     /locus_tag="P5316_19020"
     CDS             3886..4674
                     /locus_tag="P5316_19020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HRR87107.1"
                     /translation="MHTLAVIMARAGSKGLPDKCSLPLCGRPLISYTIGHAQEARLVS
                     AIVLTTDSATTKSVAQAAGIRVIDRPVELAGDTASVVDTVRHAVEVYEQQTRHKVDIV
                     VILYGNIPVRAPGIVDRCVDHLIRAGCDSVRTVAPVTKQHPDWIHRLEGDKLVQFRPN
                     TIHRRQDLEPLYYHDGAVIAVKRDSLFTPEAFENPHGFFGRDRRAVVQNPEDAVDVDT
                     RVDLFLAEAILRARGEAAVADAPAAACRRPPFTAAAVHTVTHDR"
     gene            4664..5725
                     /locus_tag="P5316_19025"
     CDS             4664..5725
                     /locus_tag="P5316_19025"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRR87108.1"
                     /translation="MTDDARKSFRIGPATVGDGSPVYVIAEAGVNHDGRVDVARELIH
                     AAANAEADAVKFQLFSADRLVTKSAATADYQKQASQGDTQRDMLARLELAREQFAELS
                     AYARYCDIEFLATPFGIDDLKFLVSIGVQAIKLASTDIVNDPLLDAAVASGLPVIAST
                     GAASEAEIARAVERFKQPGAGPLALLHCVSCYPTPEAQANLGAIGALARRFDCLTGFS
                     DHTESVSMGGYAAAAGARIIEKHLTLDRSRPGPDHAFSLEPTAMAEYIRQIRYVERLL
                     GDGSVGSQECEDEVRRLARGNIVSTRDIRAGEVLNAEMLTIKRAGGGLWASELQTIIG
                     RRAKVDIPADTAIQREWVS"
     gene            5722..6921
                     /gene="neuC"
                     /locus_tag="P5316_19030"
     CDS             5722..6921
                     /gene="neuC"
                     /locus_tag="P5316_19030"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRR87109.1"
                     /translation="MTTNRRQERKRRSRNLRVAVVTGTRAEFGLLEPVLRAMQRRPRL
                     DPRLIVTGMHLLPQFGRTVDQIRAAGWRVHATVRMQSGRGVSEEPEALAHGISGIARA
                     LDRLDCPVALVLGDRIEAFAAACAATVARRVLCHIHGGDRALGDLDDIYRDAISRMAH
                     VHLAASQEAVLRLRRMGEEPWRIHLVGAPGLDDIRAFHTRWGQRARRTNVRLQELIGP
                     LAGRSYAVVIQHPIGRSDSAEAATMRRVLKSVEAADLDGVIIFPNSDAGHKGIVREIA
                     RWQKRRQWLVFRSLPREDYLLLASHAAVLVGNSSSGIIESATLGVPAINIGPRQEGRL
                     RCGPSVVDVPDRSQAIRRAVARAARAARPAQARSVYGDGRAGERIADILGRLRLGDRL
                     IRKRLMF"
BASE COUNT         1314 a         2133 c         2161 g         1320 t
ORIGIN      
        1 caggtccatg ccgtggcaag acggctggac ggcgtgcagg ccgtccaagc cgatctcgat
       61 catcgccggg atcagcgggg caaacccacc gcagcagtgc aacatgacct tgaggccgta
      121 cgaatggccc aactcgacca gccgccgcag gtggggcagg cagaactgcc ggaataggcc
      181 ttcgctgagc agcggtccgg tctggctgcc aaagtcgttg ccgatgaaga agatgtcgat
      241 cacatcaccc gcggcctcga aaatgcgttc gctgaccgct gcgtaatagt ccacgatatg
      301 ccggaagacg gtgtgaaccg catcgggctc gtcgtacatc cgcagcatga gattctccat
      361 acccagcagg tcgatggcat cgtgccagaa aggcgaccag tcgccgccga ggatcgcgta
      421 ctgccggttg taccgcaggg cctcgtcgcg gatggccgag acgtccatcc agtccggctg
      481 gggccaggga taatcatgga tctgctcgat ggtcgcttcg gccagcgggt gactcatcgg
      541 ttgaccgtag cccatgccat gccgttggat tccgaagggc gtggcataga tcgcaccggg
      601 ttgcagatgc acctccggcc cgacgtacct ggtccagacc cggcgaaaat catcaccgaa
      661 tcgaatgcga agggcctcat cgtccaagcc gagttgcttc ttggccttgg cccagaattc
      721 cggggacgaa ccacaccagg ccgggacgtg gtccggctcc acgtggtcaa aggccgccag
      781 cactcgttca cgcgaggtca atcccaatgg aagatctcct ccatctttgc ccaccactcg
      841 ccgttgacgc gggtcggcag cggctcctgg caaggcatgc acaccgccca ccattgttgt
      901 gtcgtcggat ctgcagccat cctggccatg tccgccgcaa agtcggtacc cacgtattcg
      961 aagtagctga acaggtagcc gtccttgtgg aagatcgagt agttgcggat gttgcacgcg
     1021 ctgatcatct tcagcacgtc gggccacacg gccgcatgca gccgcttgta ctcctccaac
     1081 ccctcgggtt tgaccttgat gaccatgccg taacggttca tgctaaatcg cctcgtcgca
     1141 ctcgcccagt tgcagaggat aacggccata ttcctgcgca agccgaacag cataatcata
     1201 gctgtcgaaa gacacgctgt ccggcacgct gtgatccgac tgcatgatgt agccgccgcc
     1261 cttggccgcg ttcagcttgc gaaggacctc ccggcggatc tgctcgcggt cattggtcgc
     1321 aagcacccgt acgtcgatat tcccgttgaa cgcccatcga cggccgtact gtctcttgag
     1381 ctctaccaca tccaggcccg ccttggcttc cagcgggttg tagccgtcga cgccggcttc
     1441 gatcatatct tcgtagatga ccgacgcgtt tccgcagccg tggtagatgg ttttcagccc
     1501 ggccgcgcga gccacgtcgc acagccgctt gagctgcggc ttgtatacgc ttcgccagta
     1561 gtcgggcgag aaaaacatcc ctcgtgtgta agcgatgtcg ccccagatgt agaggccggt
     1621 cagccgaccc ttcgccgcgg caatctcagc ctcggcaatg cccaccagaa agtcgcccaa
     1681 ccgctcgatg aaccgggcca gccgctcagg ctcctcggcc atcttgtaca gggcgttttc
     1741 ggtcccgatg atccgccaga gcatctcgtg cggctcgcac accgctccaa aaacgcagaa
     1801 gtcatcggcg taagctttga cccggtcaat ccagggtggc aagttgagat tgaggctgtc
     1861 gcccaccgag ttgatctggt cgtccaaggc cgcgaaataa cgccgctcat cgtgcggatc
     1921 gtcgaaccgc agggcctcca tctgctcgaa actcgtgtgc gcgaagtcca cgtacatcgg
     1981 catggggcag gaggcgtgca cttcgatcgt cgccccgaaa cccgtcttga cagttctgat
     2041 ctcctgtgtc tcgcggacga tctggatgcc tgtcaggtgt gggtccatgt tgggcgtgat
     2101 gaccaccatg tccaggtccc agtggcggta ggggtcgaag ccgtcgccga tcttgccctc
     2161 gcgcctggcc cggcggatga agttggtcca gaagaactcg ccgaccggaa cgcgatcggt
     2221 ctcccgatgc tccaacgcag ccatgatgcg gtcgatcttg acttgggcat gactcacaag
     2281 aatggtctcc gagctctggg ccgttaccaa catgtctgtg gatgcggagt ctacccattg
     2341 atatcgggcg ccacaatgct tggccggcag ggagctgggc atgaacgacg tgaagggggc
     2401 gaggaaaacg aaagccgtgc gtcgcgacgg gacaggacat cggtcacgct gagaagcggc
     2461 tgtcagcaaa tcgaagacga tgttggcgtg gtcatcggca gaacaaacca cgaggatcct
     2521 gaaatgaaca tgacaaacaa ggtgacgtgg acggtgctgg ttggtgtttc ggtcatggca
     2581 agtgtgccgg tgaagctcgc gcagacaacg caggaggaca agacctgtct ccaggcgtcg
     2641 gccgaggacc tgcagtggtg ccgggaagct cgcttcggcc tgttcgtctg ctgggggccg
     2701 gtgacgctca aaggcaccga gatcggctgg tcacgcggcg gcgagcgccg cggcgccggc
     2761 gagaacaaga acggaatccc ggttgaggtg tacgacaacc tctacaggca gtggaagccg
     2821 acgaagtttg atgcccggct gtggtgtcag gccgcgaagg acgccgggat gaagtacttg
     2881 atcttcctcg tcaagcacca cgacggctat tgcctgtacg atacaaagct cacagactac
     2941 aggagcacca gccccgaggc ggcatggcag cacgacgtga tggccgacct aaccaaggct
     3001 tgccgggagt cggggctcaa gctgttcatc tatgattccc aaccggactg gcatcatccc
     3061 gactaccaca ccgcgcatca tcatcgctac atcgagtatc tccacggaca gattcgcgag
     3121 ttgctgacca actacggccg gatccacggt ctttggttcg acggcttggg aggtaaggct
     3181 gatgactggg acgccccgac gctcttcaag ctcgttcgcg tgtttctgcc ccacatcatc
     3241 atcagtaacc gttgccgcct gcccggcgac ttcgacacac ccgagcagaa aatcggcaag
     3301 ttccagactc acaggccgtg ggaatcctgt attaacctcg gcatgcagtg gtcctggaag
     3361 cccaacaacc ccatcaagtc attcaaggaa tgcatcgggt tactcgtgcg ctgcgcaggg
     3421 ggcgacggca acctcgccct caatgtcagc ccgacacccg atggtgattt cgaacctcag
     3481 cagctcgatc aactgaagga aatcggcggg tggctggcaa ggtacggcga gagcatctaa
     3541 ggtactcgcg gcggtccgtt ccagccgggt gattggggtg cgagcacaca ccggggctct
     3601 acggttgact tgcatgtgct gaaagcggac actgccccgc ttgtcctgcc cgcgttgccg
     3661 tccccaatac gtggttcttc gatgctgacg ggtggcaggg tttctgtcgt tcaaactgac
     3721 gaaaccacga atgtgactct gacccccgag gcacggcagg cggacgatgc aatcgtggtc
     3781 ttgcagctgg aaaaccgtat cggcggccct tgagagccac ctgcagacag ggcgagtgga
     3841 cccggggttc aagcaggtga cggttatcgg ccgatctatg tatcagtgca tacgcttgca
     3901 gtcatcatgg cccgggctgg cagcaagggc ctgcccgaca agtgcagtct gcccctctgc
     3961 gggcggccgt tgatctcata caccatcggg catgcccagg aagcccgcct cgtcagtgcc
     4021 attgttctga caaccgactc ggcgacgacc aagagcgtcg cgcaggcggc aggtatccgg
     4081 gtgatcgacc gtccggtcga actggccggc gataccgcct ccgtcgttga cactgtgcgc
     4141 catgccgtcg aggtctacga gcaacagacc cggcataagg tcgacatagt ggtcattttg
     4201 tacggcaaca tcccggtccg tgcacccggc atcgtcgatc gttgcgtgga ccatctcatt
     4261 cgcgcgggtt gcgactcggt tcggaccgtc gcccccgtga ccaagcagca tccggactgg
     4321 attcatcgcc tcgaaggtga caagctcgtc cagttccgcc ccaataccat tcaccgtcgg
     4381 caggatctgg aaccgctgta ctatcacgac ggcgcggtca tcgccgtcaa gcgagacagc
     4441 ctgttcaccc cggaggcatt cgagaaccca cacggtttct tcggccgcga ccgtcgcgcc
     4501 gtcgtgcaga accccgagga tgcggtggac gtcgataccc gagtcgacct gtttctggca
     4561 gaggccatcc tgcgggcacg cggagaagcg gccgttgccg acgccccggc agcggcctgt
     4621 cgacgcccgc cgtttacggc cgctgcggtc catacggtta ctcatgacag atgatgctcg
     4681 caagtcattc cggattggcc cagcaacggt cggcgacggc agccccgtgt atgtgattgc
     4741 cgaagccggc gtcaatcacg acggccgcgt tgatgtcgcg cgcgaactga tccacgccgc
     4801 agccaacgcc gaggccgatg ccgtgaaatt ccagttgttc tccgccgatc gcttggtcac
     4861 gaaatcggcg gccacggctg actaccagaa acaggcctct cagggtgaca cgcagcggga
     4921 catgctggca cgcctggagt tggcgcgtga gcaatttgcg gagttgtccg cgtacgcccg
     4981 ttactgcgac atcgagttcc ttgccacgcc gttcggcatc gacgatctga agttcctcgt
     5041 gtccatcggt gtgcaggcga tcaagctggc ttcgaccgat atcgtcaacg atccgctgct
     5101 ggacgcggcg gttgcgtccg gattgcccgt aatagcctca accggggccg ctagtgaggc
     5161 ggagattgcc cgggccgtcg aacgattcaa gcagcctggc gctggcccat tggccctgct
     5221 tcattgtgtg agctgttacc ccactccgga agcacaggcc aacctcggag cgatcggtgc
     5281 gttggctcgc aggttcgatt gtctcacagg tttcagcgat cacaccgaga gtgtcagcat
     5341 gggcggctac gcggccgcgg caggcgctcg catcatcgag aagcacctga cgttggaccg
     5401 ctcgcgaccg gggcccgacc atgcattttc cctcgaaccg acagccatgg ccgagtacat
     5461 ccgccagatc cggtacgttg agaggctctt gggcgacggc agcgtcggat cgcaggagtg
     5521 cgaggacgag gttcgccggt tggctcgcgg caatatcgtc tcgacgagag acatccgggc
     5581 gggcgaggtg ctcaatgccg aaatgctgac gatcaaacgg gcaggtgggg ggctttgggc
     5641 atccgagttg cagacgatca tcgggcggcg ggccaaggtc gacattccgg ccgatacggc
     5701 catccagcgg gaatgggttt cgtgaccacg aatcgcaggc aagagcggaa gcgacgatct
     5761 cgcaatcttc gcgtggcggt cgtgaccggt actcgggccg agttcggtct tctcgagccc
     5821 gtgctgcgcg cgatgcaacg ccggccgcgt ctcgatcccc gactgatcgt caccggcatg
     5881 catttgctgc ctcaattcgg ccggacggtt gatcagattc gggcagccgg ttggcgcgtc
     5941 cacgcgacgg tgcgcatgca atcaggtcgc ggcgtaagcg aagagcccga ggccttggcc
     6001 cacggcatct ccggaattgc ccgggctctc gacaggcttg attgccccgt agcgctggtc
     6061 ttgggagacc ggatcgaggc ttttgcggct gcatgtgcgg ctactgttgc gcgtcgcgtc
     6121 ttgtgtcaca tccacggcgg cgaccgggca ctgggcgact tggacgacat ctaccgcgac
     6181 gcaatcagtc gcatggccca tgttcatctg gcggcctcgc aggaagccgt tcttcgcctg
     6241 cggcgaatgg gtgaggaacc ttggcggatt cacctggttg gggcgcccgg gcttgacgac
     6301 attcgagcat tccatacacg gtggggtcaa cgtgcccgac gcacgaacgt gcgtttgcag
     6361 gaactgatcg gtccgctggc cggacggtcc tatgccgtgg tgatccagca tccaatcggc
     6421 cggtccgatt cggcggaagc cgccaccatg cggcgagtgc tgaagtcagt tgaggccgcc
     6481 gatcttgacg gagtgatcat ctttccgaac agcgacgcgg gtcacaaggg aatagtccgc
     6541 gagatcgccc gatggcagaa acgccggcag tggctggtat tccggtcgtt gcctcgtgaa
     6601 gactatctgc tgcttgcctc gcatgctgct gtgttggtgg gcaactcttc cagcggcatt
     6661 atcgagtccg ccaccttggg cgtacctgcg ataaatatcg gacctcggca ggagggccgt
     6721 cttcggtgtg gcccgtcggt ggtcgatgtg cctgatcggt cccaggcaat tcgcagggcg
     6781 gtggcacgcg ccgctagggc tgcccgtccc gctcaggcaa ggtctgtcta cggtgacggg
     6841 cgggccgggg aacggattgc ggatatcctc gggcggctcc gtctgggtga tcgcctgata
     6901 cgaaaacgcc tgatgttctg atccttct
//
LOCUS       DAOTPW010000282         6909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9525_length_6909_cov_4.560986, whole genome shotgun sequence.
ACCESSION   DAOTPW010000282 DAOTPW010000000
VERSION     DAOTPW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9525_length_6909_cov_4.560986"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="P5316_19035"
     CDS             complement(<1..879)
                     /locus_tag="P5316_19035"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRR87110.1"
                     /translation="MIDIKAIRENPDRFKTAARNKRISCDIDAVCALDDRRRSLQTEM
                     DRIKHQQNEISDQIANHRNPKSKYFQQALAAGRTEIELKAEAERLVQQVAEMKARAKQ
                     LESEHREVCEQLDAALLTVAQPAADEVPIGKDDTENVEVRRVGEIPKFDFEPQDHVTL
                     MTRLGMLDVDRGVKLAGTRNYVLRGDGSALHQAVLRLALEMMVQRGFVQMTVPVLVRD
                     AMMVGTGYFPGGEEQAYRCERDAMSLVGTAEVPLTAYHCDEILSEDELPKKYVACSTC
                     FRREAGAAGKDTYGLYR"
     gene            complement(1391..2440)
                     /locus_tag="P5316_19040"
     CDS             complement(1391..2440)
                     /locus_tag="P5316_19040"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRR87111.1"
                     /translation="MYNRLFAWKGSFALAPNDSHGCYVSNEFPCFQVNPDRLDGMFLW
                     RYLSQEPVWTEALGLSTGGTPTSRNRLKEEKLLQMTIPLPPLSEQRRIVAKIDQLAAK
                     IEEARELRSTLEDEGDVLVRAETHRLFHVEQDRPPVGWSMAAVGDLGPKGAETVQTGP
                     FGAQLHKSDFVPEGRPVLAIGNVQWGYLDTRTIDHVTEAKAAELARYILADGDVLFTR
                     SGTVGRSAVVPADADGWLMTGHILRIRLNRTRCEPQFLFHGFRGSRDMQEQVEGSIPG
                     ATRAGFNTALLSGVRLPLPPIPEQRRIVEYLDGLQAKVDRLKELQAATAAGSRKASNY
                     PGRQKSQDELSVIVG"
     gene            complement(3053..3946)
                     /locus_tag="P5316_19045"
     CDS             complement(3053..3946)
                     /locus_tag="P5316_19045"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HRR87112.1"
                     /translation="MYFFYIDETGNRDPRLTIPRKDGTTVAGDWLYVLTAVSLFEKRW
                     HGFEKTLNRRKGFLMNRIFKETGTRLQLADCEVKSNWVRQPKERTQHPFLRHVTDEEM
                     TGLVDLFYQQLEHHKMHIFSVLVDKRHRPDYMDQEKLHRKSWELLLELVQRFMASTHG
                     KHQALMVTDDVQIQVNRSLAMKHAYIMDQGTASGMWLTHICEMPMFVRSELSNGVQLA
                     DLCGYNIYRAFRSGDLDYAFFRRIAPKIWSRHYPLRTGKAGFSGLYVFPGNSPLRGLV
                     DALENEKAPPNKPDGASEHNQ"
     gene            complement(3971..4120)
                     /locus_tag="P5316_19050"
     CDS             complement(3971..4120)
                     /locus_tag="P5316_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87113.1"
                     /translation="MLSVNLDIKNPNAAEDLEHLPPEKLVEDILAKEKLIIEIVGEIK
                     GVLRS"
     gene            complement(4204..4425)
                     /locus_tag="P5316_19055"
     CDS             complement(4204..4425)
                     /locus_tag="P5316_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87114.1"
                     /translation="MPRGKADRKESPQTTTQQLGSLIKSARDIMRKDKGLSGDLDRLP
                     MLTWVMFLKFLEDMEQIEASRAKMRGEAA"
     gene            complement(4430..5482)
                     /locus_tag="P5316_19060"
     CDS             complement(4430..5482)
                     /locus_tag="P5316_19060"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRR87115.1"
                     /translation="MSAADEELVFSSVSERDVDFMLVEEFRCSIAFSQWFAGRILPPD
                     EQEHVRRLELPKVLHSVRANGPGGGESDIVLMFGPSAQTKLLVLVENKIDAVFQEAQA
                     QRYQTRAREAVARHEAERAICVLVAPNEYVETSDDASHFDAIVTYEEISEFFNEQVSA
                     GDAEYARRQQHRRYLIDCAIGRYRRGYAPVPSPETTRIWEIYYQRACAIAPGLKMRQP
                     GCKPAYSSFIRFGEAIAQHPGLPRMVIKHKLCYGNVDLELPGWASCSGLVTPVLEPAL
                     EPGMKLRLAGKSLAVNIIVPVFNMDAEPGDQIKKFDLGLEAALRLQSWCEQHLPILRR
                     CAALIDQKAQGMQNEE"
     gene            complement(5492..6403)
                     /locus_tag="P5316_19065"
     CDS             complement(5492..6403)
                     /locus_tag="P5316_19065"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRR87116.1"
                     /translation="MANQVDPEHLGKMMQRLLRELKAIGQPTQFGELLRRTEPELGLT
                     DYDRELLAKSNYPRYYALLQWLSVDCVKAGFLSKSGGRWALTDQSDEAMKLPPAGFVR
                     VARSRYSGWRQKNSLGEESQENVATVPGAENEEAVAIQATYEQVTERAREQIEEHISR
                     LGPYDFQQLVAELLMGMRYHVPYIAPPGPDGGIDIVAYNDPLGTGTPRIRTQIKHRVN
                     NKVTVAEVRALEGVLRKDGDMGLIVSSGGFTSESLREIQISSKHIESMDLDRLITLWQ
                     EHYDRIRERGKLILHLVNVFLLAPVEE"
     gene            complement(6414..6836)
                     /locus_tag="P5316_19070"
     CDS             complement(6414..6836)
                     /locus_tag="P5316_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87117.1"
                     /translation="MPNEADTCRRFVVPRLQAAGCDCEPHRLHEQVTFTDGRIIVTGR
                     RGRRRPGKRADYILRYRPDMPIAVVEAKPTYAGPGDGLQQVAEYAEHGTAQFVIPDLL
                     QLPPINRHGNVIEIARQFGGEEQLIEAIRQLQTLLYAA"
BASE COUNT         1236 a         2097 c         2022 g         1554 t
ORIGIN      
        1 ccgatagagg ccgtacgtat ccttgccggc ggccccggcc tcgcggcgga agcatgtgct
       61 gcaagcaacg tactttttgg gcagttcatc ctcgctgagg atttcgtcac aatggtaagc
      121 cgtgagcggc acctcggcgg ttccgacaag gctcatggcg tcacgttcgc agcggtaggc
      181 ctgctcttcg ccgccgggaa agtagccggt gcccaccatc atcgcgtccc ggaccagcac
      241 cggaacggtc atctggacga agcctcgctg gaccatcatc tcaagagcca ggcgcaggac
      301 cgcctggtgc agggcgctgc cgtcgccgcg aaggacgtag ttgcgggtgc ccgcgagctt
      361 gacgccccgg tcaacgtcga gcatgcccag tcgggtcatc agcgtgacat gatcctgggg
      421 ttcaaagtcg aacttcggga tctcgccgac gcgtcggacc tcgacgtttt cggtgtcatc
      481 cttgccgata gggacctcat ccgccgccgg ttgggcgacc gtcagcaggg cggcgtcaag
      541 ctgctcgcag acttcgcggt gctcggattc gagctgcttg gcccgggctt tcatttccgc
      601 cacctgctga acgagtctct ccgcctcggc cttcaattcg atctcggtgc gaccggcggc
      661 cagggcctgt tgaaagtact tgctcttggg attgcggtgg ttggcgatct ggtcgctgat
      721 ctcgttctgt tgatgcttga tgcggtccat ttccgtctgg aggcttcgtc gcctgtcgtc
      781 cagcgcgcag acggcatcaa tgtcgcaact gatgcgcttg tttcgcgccg cggtcttgaa
      841 acggtccggg ttttcgcgaa tcgccttgat gtcgatcatg tttggtcctt gctgtctgtc
      901 ctgagacaaa aaagccctga ggcaccatcc ggcgtctcag ggctttcagc atcgtattgt
      961 tttacgtcag ctcatgccct gcagcgccgg tacgggctaa acgaatacca gaagtagtag
     1021 ctgctgaaca gggtgctacc ggcaacgggg gctgtaccgt tgtgcggacg ttccagtgat
     1081 tggttgcggt caatcgccat ggtcagtctc agcctctggc tctattgttg cacgggccgc
     1141 aggccatgtc aagcccgttg tcggccggcc gctgggtcca tgacggtctg ggcggggtgc
     1201 cactggcagc cgaggtcgaa ctctcccgct taccaagggg catgtcccga ggatgtcgag
     1261 ggggagttgg agggggtcgc attctcctct tcggacgggt ggcccaccca ttcgcgacag
     1321 cgaatggatg gggtctcgat aatgccttct gagccacgcg gtttcctgtt ccgcccgtcg
     1381 ccaggccttg tcacccgact atcacggata attcatcctg agacttttgt cgacctggat
     1441 aattcgatgc ctttctcgat ccggcggcgg tggcggcctg gagttccttc agccggtcca
     1501 ccttggcttg caggccgtcc aggtactcca cgatgcgacg ctgctcgggt atcggcggaa
     1561 gcggtagacg gacaccactg aggagcgctg tgttgaaacc ggctcgggtt gcccctggga
     1621 ttgagccttc gacttgttcc tgcatgtccc ggctacctcg gaagccatga aagaggaact
     1681 gaggctcaca gcgtgtcctg ttcaaacgaa tccgcaggat atgccctgtc attagccagc
     1741 cgtccgcgtc ggcggggacc acggcggatc gacccacagt gccggatcgc gtgaaaagca
     1801 catctccgtc ggccaagata tagcgggcga gctccgccgc ttttgcctct gttacgtgat
     1861 ctattgtccg tgtgtccagg tagccccact gtacgtttcc gatcgcaagc acaggtcggc
     1921 cttcgggaac aaagtcactc ttgtggagct gtgccccaaa ggggcctgtc tgaacggttt
     1981 cggctccttt tggcccgaga tctccgacgg cggccatact ccatccgaca ggtggcctgt
     2041 cctgttcgac atggaaaagt cggtgtgtct ctgctcggac caatacatca ccttcgtctt
     2101 ccagcgtgct tcgcagctcc cgggcctctt cgatcttggc cgccagttgg tcgatcttgg
     2161 ccactatccg ccgctgctcg gaaagagggg gcagggggat ggtcatttgc aggagcttct
     2221 cttccttcag ccggtttcgg cttgtcgggg ttcccccggt gctcaggcca agggcctcag
     2281 tccagaccgg ttcctggctc agatacctcc agaggaacat cccgtcgagc cggtcggggt
     2341 tgacctggaa acatggaaac tcgttggaga cgtagcaccc atggctgtcg tttggtgcaa
     2401 gagcaaacga gcccttccag gcaaagaggc ggttgtacac aaagtcccct gttccacgcg
     2461 gtacaccttg ggtgcgcgga tctgggaacc cgaaagggtg tccttcacgt aaaggccgag
     2521 cgcgtaccat ttcgcgccca gcagtcgata ttccttgctc gggtcaaccg actccgggcg
     2581 actcaccggt tcgagtacct cccccagcgg caccatcggc cagccgttcg tcattgcccc
     2641 tcctctgtgg ccatcgagcc gccctcgggg caggccgaac gaataatctg agaaatatgt
     2701 tgacagaagt ccatatatga attacactta tgatgccagt ggatgaaact cggcttcatg
     2761 ccgtgtgatc gatcggcggc agtcggtcga tcctatatcc ccggtggcta ttccgcacca
     2821 ccgtgctgga atcgagctgt cggcgcgcca acgcagtgcc cggtgctcct gtaactcatt
     2881 actcaagaaa gagttgtgtg cgaccggcca gcctgaggcc cgtgttgcgg ggcactggtt
     2941 ctgcctgtca ctccacgggc gatggcaagt tgttgtcatc atggaaggat accgatataa
     3001 ttcatgtgtc cggtgccgct tgtcggctcg atagggttcg ccccgccgga catcattgat
     3061 tgtgttcaga ggccccgtca ggtttgtttg gcggggcttt ttcattctcc agcgcatcga
     3121 ccaaaccacg caagggactg tttccgggaa agacgtacag ccctgaaaag ccagccttgc
     3181 ctgtccgtaa cgggtagtgc cgcgaccaga tcttcggggc gatcctgcga aagaaagcat
     3241 agtccagatc gccacttctg aacgcgcggt agatgttgta cccgcacagg tcggccaact
     3301 ggacgccatt tgacaactcg cttcgaacga acatcggcat ttcgcagatg tgcgtaagcc
     3361 acatgccgct ggccgtgcct tgatccatga tgtaggcatg cttcatagcc aatgatcggt
     3421 tgacttggat ctggacatca tcggtcacca tgagtgcctg gtgcttcccg tgggtgcttg
     3481 ccatgaaccg ctgcacaagt tcgagcagca gttcccacga cttgcggtgc agtttctcct
     3541 gatccatgta atccggccgg tgccgcttgt cgaccaggac gctgaagatg tgcatcttgt
     3601 ggtgttcgag ttgctggtag aaaaggtcca ccaatccggt catctcttca tccgtcacat
     3661 ggcgaagaaa cggatgctgt gtgcgctcct tgggttggcg cacccagttc gacttgactt
     3721 cgcagtcggc cagttgcagg cgcgtaccag tttccttgaa gattcggttc atcaggaagc
     3781 ccttgcgtcg attgagtgtc ttctcgaaac cgtgccagcg tttctcgaag aggctcaccg
     3841 ccgtcaggac gtacagccaa tctcccgcca cggtggttcc gtctttccgc ggaatcgtca
     3901 agcgggggtc tcggttgccg gtttcatcga tgtaaaagaa gtacacgcga ttctgacctt
     3961 tccttcagag ctatgaccga agcacgccct tgatctcacc tacaatctcg atgatcagct
     4021 tctccttcgc caggatgtcc tccaccagct tctccggcgg caggtgctca agatcctcgg
     4081 cggcgttggg gttcttgatg tccaggttga cggacaacac gttgccgtcg gcatcgagct
     4141 tgatcaggtc cttggccttg accttccacg cccggtcgtt ctccttgcgc ctcggccacc
     4201 acttcaggcg gcctcgcccc gcatcttggc ccgcgacgcc tcgatctgct ccatgtcctc
     4261 gaggaatttc aggaacatga cccaggtcag catcggcagg cggtccagat cgccggacag
     4321 gcccttgtcc ttgcgcatga tgtcgcgggc gctcttgatc aggctgccga gctgctgggt
     4381 ggtggtttgc ggcgactcct tccgatcggc ttttccacgt ggcaagacgt tactcctcgt
     4441 tctgcatccc ttgggctttc tgatcaatca gggcggcgca cctccgaaga atcggaaggt
     4501 gttgctcgca ccatgattga agacgaagcg cggcttcaag acccaaatcg aacttcttga
     4561 tctggtctcc gggctcggca tccatattaa acaccggaac gatgatgttc acggcgaggc
     4621 tcttacccgc cagtctgagt ttcatgcccg gctcaagcgc cggttccagc acgggggtta
     4681 cgagtccgct acaggaagcc cagcccggca actcaaggtc tacgtttcca tagcagagct
     4741 tgtgtttgat caccattcgt ggcaagccgg gatgctgtgc aatcgcttcg ccgaatcgga
     4801 taaagctgct gtacgctggc ttgcagccgg gttgacgcat tttcagtccg ggcgcgatcg
     4861 cgcaggctcg ttggtagtag atctcccata tacgtgtggt ctcagggctc ggcacggggg
     4921 catagcctcg ccgatacctc ccaatcgcgc agtcaatgag ataccgccgg tgctgctgac
     4981 ggcgggcgta ttccgcgtcg cctgcggata cttgctcatt gaagaactcg ctgatctcct
     5041 cgtaggtcac gatggcatca aaatgcgagg catcgtctga cgtctcgaca tactcgttgg
     5101 gcgcgaccag aacgcagatc gccctctcgg cctcgtgtct cgcgacggcc tcgcgggcgc
     5161 gtgtctggta gcgctgggcc tgcgcttcct gaaagaccgc atcaatcttg ttctcgacca
     5221 ggaccagcag cttcgtctga gccgatggac cgaacatcag cacgatgtct gattcgccgc
     5281 ctccggggcc gtttgcgcgc acgctgtgaa gcaccttggg cagttcaaga cgccggacgt
     5341 gttcctgctc atccggtggc agtatccggc cagcaaacca ttgagagaag gcgattgaac
     5401 aacggaattc ctcgacgagc atgaaatcca catcccgctc agacacggac gaaaagacca
     5461 gttcttcgtc tgctgcgctc acggcgtgcc ctcactcctc gacgggtgcc agaagaaaga
     5521 cattcaccag gtgcagaatc agctttcctc gctcgcggat gcgatcgtag tgctcctgcc
     5581 acagagtgat cagccggtcg agatccatgg actcgatgtg cttgctggat atctgtatct
     5641 ctcggagaga ctcactcgtg aatcctccgg aagacacgat caggcccatg tcaccgtcct
     5701 tgcgaaggac accttccagg gcacgaacct ccgccacggt aaccttgttg ttgacccgat
     5761 gcttgatctg cgttctgatt cgtggggtcc cggtgcccag tgggtcgttg tacgcaacga
     5821 tatcgattcc cccatcgggg ccggggggtg cgatgtacgg gacatggtag cgcatgccca
     5881 tgagcaactc cgccacgagc tgctggaaat cgtagggccc gaggcggctg atgtgctcct
     5941 cgatctgctc acgagcccgt tccgtaacct gttcgtacgt ggcctggatg gctaccgctt
     6001 cttcgttctc ggctcccggt acggtggcaa cattctcctg tgactcttcc cccagagaat
     6061 tcttttgcct ccagccagaa taccgtgatc tggctacacg cacgaacccc gccggaggta
     6121 gtttcattgc ctcgtcggac tggtcggtca atgcccaccg gcctccagac ttcgagagaa
     6181 agccagcttt gacgcagtcg acagagagcc actgcaacaa ggcgtagtat ctcggatagt
     6241 tgctctttgc gaggagttcg cggtcgtaat ccgtaagacc aagctcgggc tcggtcctcc
     6301 gcaacaactc gccaaactga gttggctgtc ctatcgcctt caattcgcgg agcagccgct
     6361 gcatcatctt cccgaggtgt tcggggtcaa cctggttcgc catttgtttc accttatgcc
     6421 gcgtacagca gcgtctgcaa ctgccttatc gcctcgatca actgttcctc gccgccgaac
     6481 tgcctggcga tctcgatcac gttcccgtgt cggttgatcg gcggcaactg caaaagatcc
     6541 gggatcacga attgcgccgt cccgtgctct gcgtactctg cgacctgctg aagcccgtcg
     6601 ccggggccgg cgtaggtcgg cttggcctcg acgactgcga tgggcatgtc cggccgatag
     6661 cggaggatgt agtcggcccg tttgccgggt cggcggcggc cccgccggcc ggtgacgatg
     6721 atacggccat cggtgaaggt gacctgctca tggagccggt gcggttcgca gtcgcacccg
     6781 gcagcctgaa gcctgggaac gacaaaacgt cggcacgtat ccgcttcgtt cggcatcgac
     6841 tactgctcgg accagcgtca gtgcgggtag atgagtctcg ctgtcacatt acgccaagca
     6901 tgtcaccgg
//
LOCUS       DAOTPW010000283         6859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9607_length_6859_cov_5.039389, whole genome shotgun sequence.
ACCESSION   DAOTPW010000283 DAOTPW010000000
VERSION     DAOTPW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9607_length_6859_cov_5.039389"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="P5316_19075"
     CDS             <1..1021
                     /locus_tag="P5316_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87118.1"
                     /translation="GVAKPGSFAQLAGMLVLACGIAGCPRVEPGLGPPEEPLPLQAVI
                     ERVNWNSGRMDFLLRGVNGSAVGRWRRSSDDTTPSTSFDMKVVLLYRKPRNLYLRMEH
                     TLGGMIEAGSNSREFWVWERAKENRYYWGEHQWLTNDAEIDLPVRPDVLLDVLGVGDL
                     PTDTTGSQGPVLWVCENGYQLVFLDYDEQGQGYISKVMEIGRREPYLVQRILYFHPDG
                     RPVMQVLLSDYRLVEGSEVRAPHKIEMNSMEAGNDNHMTLRFPSLKRFDQPAADQLFV
                     SPMQKGGAGLSYVRRVDGWKAQRQPVSRPAPKTAPAADTATSRFPQTSRPCEPATQPD
                     AVETR"
     gene            1018..2769
                     /locus_tag="P5316_19080"
     CDS             1018..2769
                     /locus_tag="P5316_19080"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRR87119.1"
                     /translation="MTDSPRFPKIICWLTALAAVGAPTAAARAERAIVQLHASGGEHV
                     LWLVTGIREPGGQTLLQWFSYLDKPDDPPRAAPVSPQLGRIARVATVQDTIHVFFADG
                     VHYSYSKSGDRRELRLPESAVPAAIAGTPTGRPPLLWALVTSHTAGRVEAEWLEQQRR
                     QASKPATRELGRSSPASSLPVIEEPKRTTQSLGGYSLVRYDGLGWRPGFDAAPEFTTA
                     ARVWLCAHENRQILLWQVDAKSDEILSSWRIDDQWRANETLRLPEAPTAAFAMSSGND
                     LLFAAIIRERINAGGLRCWVWRMSPPNTGMRGEWLAVSSPVLDDGKPLEMDAGAAVAA
                     FGDYLAVVRPAGNKAEAGLWSLKDGKPAKPFVDVPTRQGEPKPPSSYRNLADVIIIGV
                     LMAVVLVIFRRRHEHTIAPAMLPAEVVVANLGKRVVAALIDMLPAMILVGWLWRTPIA
                     DFWDDLSTIGLLGRFPNDIPVPHALFWAGLAFRLIYAAYCLVFELFWSATPGKRLMGF
                     SVVTESLDRPSRLQVLARNLTRPLELEPAMIFFLAIVFFTPYRQRIGDLLARTVVIEG
                     TPPAKTNSQELQDDADQ"
     gene            3059..3913
                     /gene="accD"
                     /locus_tag="P5316_19085"
     CDS             3059..3913
                     /gene="accD"
                     /locus_tag="P5316_19085"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRR87120.1"
                     /translation="MADPDVPSTTPAATSPPVLNRVDVPAGLWTQCPSCQATVYQKAL
                     EENLHVCPECGYHHRIPARTRIRQLVDPDTFEEFLGDLVSTDPLKFSDRGKYKDRLKQ
                     AQEKSGEIEAVVTGKAFIRGRPVILAVMDPFFIMASMGAVVGEKVTASLERATDENIP
                     FVIVTASGGARMQEGMVSLVQMAKTASAVARMDDAGGLYIVVMTDPTTAGVAASFASL
                     GDIHLAEPGAMIGFAGPRVIENTIKAKLPKGFQSAEFMLEHGFVDRIVHRRNLRTEIA
                     RIIDYAGK"
     gene            4136..4465
                     /locus_tag="P5316_19090"
     CDS             4136..4465
                     /locus_tag="P5316_19090"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRR87121.1"
                     /translation="MANTDTSPIYSSLKDDPDMVELIEEFVGNLQHRVQAIEQAVASN
                     NPAELVRLAHQLKGASGGFGFETIGAMAAALEQSARAATCVSDVAAEVAELVSMCRRA
                     TAQPAPW"
     gene            complement(4863..5174)
                     /locus_tag="P5316_19095"
     CDS             complement(4863..5174)
                     /locus_tag="P5316_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRR87122.1"
                     /translation="MAKLTKSATAKTLAEKVGITRKQAMLFMEELATLAYKEAKNSFT
                     LPGLGKLVLVNRKARMGRNPATGEAIKIPAKKVVKFRVAKACKDAILGTAGKKPPKKK
                     K"
     gene            complement(5696..>6859)
                     /gene="argJ"
                     /locus_tag="P5316_19100"
     CDS             complement(5696..>6859)
                     /gene="argJ"
                     /locus_tag="P5316_19100"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRR87123.1"
                     /translation="AGAVMVSLCMIKQVNKLDIAVLVADDICPAAAMFTSNRFCGAPV
                     IVGREHTRRGRLRALVVNSGCSNVATGKRGIADARAMCRRLAESIGAKPIEVLPSSTG
                     VIGRFLPMDKILPGIDAALETLSASAEAGERFAQAIMTTDTRMKQACERVRIGRTLIT
                     VAGCCKGSGMIAPNMATMLAFLTTDAGVPPAVLKRLLKEAVAPTFNRVTVDECQSTSD
                     TVAIMASGAAGDLNTRQTASFSKALRAVCESLAHQIVRDGEGATKVLEVIVRGAKSPA
                     DAHAVARAVAVSPLVKTAVHGGDPNWGRIVQAIGATSVTFRPEKVTVRLGKDLLFAKG
                     APAPRLDLVKLAEAMRQPHVSLMIDLGAGKAADRVLTCDLSREYIAINADYTT"
BASE COUNT         1277 a         2083 c         2118 g         1381 t
ORIGIN      
        1 gggagtggcc aagcctggat cgtttgccca acttgcgggg atgcttgtct tggcctgcgg
       61 gatcgccgga tgcccacgtg tcgagcccgg gctcggcccg cccgaagagc cgttgccgct
      121 gcaagcggtg attgagcgag tgaactggaa cagcgggcgg atggatttct tgttacgggg
      181 agtgaacggg tcggctgtcg ggcggtggcg gcgcagctct gacgacacca ccccaagcac
      241 ttccttcgac atgaaagtag tcttgcttta tcgaaagccg cgcaaccttt atttacggat
      301 ggagcacacg ctgggcggca tgatcgaggc cggctccaac agccgtgagt tctgggtctg
      361 ggagcgggcc aaggagaatc gctactattg gggcgagcac caatggctga cgaatgacgc
      421 tgagattgac ctgcccgtgc ggccggacgt gttgcttgac gttctgggcg tcggcgattt
      481 gcccactgat actaccggct cgcagggccc ggtactctgg gtgtgtgaga acggttacca
      541 gcttgttttc ctggattacg acgaacaggg acaggggtat atcagcaaag tgatggagat
      601 tggccggcgc gaaccctacc tggtgcagag aattctctat tttcatcctg acggccggcc
      661 ggtgatgcaa gtgttgctgt ccgactatcg gttggtcgaa gggagtgagg tgcgcgcccc
      721 ccacaagatc gagatgaatt cgatggaagc gggaaacgac aatcacatga ctctgcgatt
      781 tccctcgttg aaacgttttg accaaccggc ggccgaccag ttgtttgttt cgccgatgca
      841 gaagggcggt gcgggcctca gctacgtgcg ccgcgtggat ggatggaaag cccagcggca
      901 accggtgagc cggccggctc ccaagacggc ccccgcggcc gacaccgcca cttcccggtt
      961 cccgcaaacg agccggccgt gcgagcccgc gacgcagcca gatgcggtgg aaacacgatg
     1021 accgactcgc cgaggtttcc gaagatcatt tgctggttga cggcactggc ggccgtggga
     1081 gcgccaacgg ctgcggcccg ggccgagcgg gcaattgttc aactgcacgc atcggggggc
     1141 gagcacgtcc tttggctggt caccggcatc cgcgaacctg gcggacagac gctgctacaa
     1201 tggttctcct atctggataa gccggacgat ccgcctcgcg cggcaccggt ctctcctcag
     1261 ctcggccgga tcgcccgcgt cgccacggtc caggatacga ttcacgtctt ctttgccgac
     1321 ggcgttcatt actcctattc gaagtcgggc gatcgtcgcg agctgcgcct gccggaatcc
     1381 gcggtcccgg cggcgattgc cggaacaccc accggtcggc cgcctttgct atgggccttg
     1441 gtcacctcgc acacggccgg ccgcgttgag gctgagtggt tggaacagca acggcgtcag
     1501 gcctccaagc cggcgacccg agagctcgga cggtcttcac cagcttcctc cttaccggtg
     1561 attgaagaac caaaacgcac gactcaatca ctgggcggtt actccctggt tcgatacgac
     1621 ggtttgggtt ggcgtcccgg cttcgacgcc gctccggaat tcacgacggc ggcccgcgtg
     1681 tggttgtgcg cccacgagaa ccgtcagatt cttctgtggc aggtggacgc caagtctgat
     1741 gagattctct cgtcgtggag gatcgacgac cagtggcggg cgaacgaaac actgagactg
     1801 cctgaggccc cgacggctgc tttcgcgatg tccagcggca acgacctgtt gtttgccgcg
     1861 atcatacgtg agcggattaa cgccgggggg ctgcgatgct gggtgtggcg gatgtcaccg
     1921 cccaacacgg gtatgcgggg agaatggctc gccgtctctt cgccggtcct ggacgatggg
     1981 aagccgctcg agatggacgc gggggctgcg gtggcggctt ttggagacta tctcgcggtg
     2041 gtccggccgg caggcaacaa ggcggaggca ggcttatggt cgttaaagga tggcaagccg
     2101 gccaaaccgt ttgtggatgt ccccacaagg cagggggaac ccaaacctcc ctcttcgtat
     2161 cgtaacctgg cggatgtcat catcattggg gtgttgatgg cggtggtcct cgtgattttc
     2221 cgcagacgcc acgagcatac catagccccg gcgatgttgc cggccgaagt ggtcgtggca
     2281 aacctgggca agcgcgtggt ggcggccctc atcgacatgc ttccggccat gatcctcgtc
     2341 ggttggctct ggcggacgcc aatcgcggat ttttgggacg acctctccac gatcgggctc
     2401 ctcggacgat tcccgaacga tattcccgta ccccacgcgc tgttctgggc cgggttggcg
     2461 tttcggctga tttatgcagc gtactgcctt gttttcgagc tattctggtc ggcaacgccg
     2521 ggcaagcggc tgatgggttt ctccgttgtg accgagtcgc tcgaccggcc cagtcgtttg
     2581 caggtattgg cccgcaacct gacccggcca ttggagctcg aacctgccat gatcttcttc
     2641 ctggcgattg tgttctttac gccttaccgg cagcgaatcg gcgatctctt ggccaggacc
     2701 gtggtgattg agggcactcc ccccgctaag acaaacagcc aggaactgca ggacgacgcc
     2761 gatcagtagg ctctgacaca gcttgccgat cgccgtcccc cagcgaagca gcctcgctac
     2821 ttttaggcag gtaaacaatc gtccgttgac cgagttgcgg tggtcggtca agggcctgtg
     2881 acttactcgc cgcgtcctgt aacgggcgac cgcgacatcc ggccgaacca ttacggaccc
     2941 ggcctgcccc ggcggatccc cggggtggac aaccgtgccc ggaagccgta ataatggagc
     3001 ccccaccggg cgtcgggagc ggtctcgtgg catccggtcg gcatgcggga gatttcattt
     3061 ggcagaccca gacgtaccca gcaccacccc ggcggccact tcgccgccgg ttttgaatcg
     3121 tgtggacgtc ccggcaggac tgtggacgca gtgtccgtct tgtcaggcga ccgtctacca
     3181 gaaggcgttg gaggaaaacc ttcacgtctg tccggagtgc ggataccacc atcgcattcc
     3241 ggccaggacg aggatcaggc agcttgtcga cccggacacc ttcgaggagt tcctgggaga
     3301 cttggtcagc accgacccgc tgaagttctc cgatcgcggg aagtacaagg atcgacttaa
     3361 gcaggcacag gaaaaatccg gcgagatcga ggccgtcgtg acgggtaagg cctttattcg
     3421 cggccgaccg gtgatcctgg ccgtcatgga tccgttcttc atcatggctt ccatgggcgc
     3481 ggtcgtcggc gagaaggtca cggcttccct cgaacgggcc acggacgaga acatcccgtt
     3541 tgtcatcgta accgcctccg gaggtgcccg gatgcaggag ggtatggttt cgttggtcca
     3601 gatggccaag accgcctcgg ccgtcgctcg aatggacgac gccgggggat tatacatcgt
     3661 ggtcatgact gacccgacca ccgcaggggt ggccgcaagc tttgcatcac tgggcgatat
     3721 tcacttggct gagcccggtg ccatgatcgg ttttgcgggt ccgcgggtga tcgagaacac
     3781 catcaaagct aagctgccca aaggattcca gtccgcggag ttcatgctcg aacacgggtt
     3841 cgtggatcgg atcgtccacc gaagaaatct gcggaccgag attgcccgga tcattgacta
     3901 cgcgggcaag taaagccctc gccgctctcg tacagcagat tctccacctt gggtggcccc
     3961 ggctttgcca tgtgtccgca taattcgcgt gggccgataa ccggccgtca gacgccactg
     4021 tgattcgtgt agtcacccgg cacctcttgt gaaccgatat aaacaatgcg tcctgtgccc
     4081 gtatcaggct caaaacgcac aattgcaggg ctcgaaaacc gcggggacaa acccgatggc
     4141 caacacggat acttcaccga tctatagctc tttgaaggac gatcccgaca tggtggaact
     4201 gatcgaggag tttgtcggca acctgcaaca tcgtgttcag gcgatcgagc aagccgtggc
     4261 ttcgaacaac cccgccgaac tggtgcgatt ggcacaccag ttgaaagggg caagcggcgg
     4321 atttggcttt gaaacgatcg gtgccatggc ggcggctctc gaacagtcgg caagagcagc
     4381 cacgtgcgtc agcgacgtcg ctgcggaagt cgcggaactg gtttccatgt gccgacgggc
     4441 aacggcgcaa cccgcacctt ggtaatcgcc aagactgatt gagcagaagc agtttcctgt
     4501 tccgcttcga ccaacggtcg gatcgtttac tccgtcgcca ccgcgatacc gacattattg
     4561 tttcgcccga agtcaacaca acaatgtggc gaaatgcggc aactgcgacc gccggaagtg
     4621 cgcggtcaga cgattgactc cccgccggcg ctactcgcct tcgcgccgat cacgtcggaa
     4681 gacgaagccg gcatcacttc cccataaaac gaatcgccgc aagaccctgg actcgcgtat
     4741 tccagtccgg agtttcgcgg cgatcggcgc cggctgctcc caaggagcct ctcaacgcaa
     4801 tggttcgttt cggctgtcat ccgtcgcgaa tcgtggcatt gcgacggccg aaccctgtgg
     4861 gcttacttct tcttcttggg aggcttcttg ccggcggtcc caaggatcgc atccttgcat
     4921 gccttggcaa cacggaactt cacgaccttc ttggccggga tcttgatcgc ttcgccggtc
     4981 gccggattgc ggcccatacg ggctttccga ttgaccagca cgagcttgcc aagacccggc
     5041 agcgtgaagc tgttcttggc ctccttgtag gccagtgtcg ccaattcttc catgaacagc
     5101 attgcctgct tgcgggtaat cccaaccttt tctgcaagcg tcttggcggt ggccgatttc
     5161 gtaagtttgg ccattacaaa cctccttgca aaagacccgg gccgcccacc ggcccgattt
     5221 gtgacgaata ttactgacct tttgtcggtt cgtccagcga aaacccttga aaatattgga
     5281 tttttttgtg gccgatgaca cgcaagcaag ttttatcaag ggttttcaca ggaggacatg
     5341 gtcgttccgt ggcaatccta aggcggtccg ccttttggtg ggccgcaacc caagcgcact
     5401 gcgatccaga gacatgtgcg caagccgcac agacgtctgg ccgtgaggtt ccaaggtcct
     5461 tccggggcgt tggacgccgt ccctgtaccc ggcaaaacgg cccctgaata tgccggactg
     5521 tatcgggcat tcgctggaaa accgtcgatt ttccgccggt tttgaaggcg tctgcttagc
     5581 tgccggttgt tcctgccgct tcgagactcg cggaaaagat cgtttggctg ctcaagagcg
     5641 cccgacatgc ggtgatgctc gatggcgagg gatgacccct gcggcagccc gaatcctacg
     5701 tggtgtaatc ggcattgatc gcgatgtatt cccgcgacaa atcacaggtc agcacccggt
     5761 cggctgcctt ccccgctcca aggtcgatca tcaagctcac gtgaggttgc ctcatcgcct
     5821 cggccagctt gacaagatca aggcggggag caggagcgcc tttggcaaag agcaggtcct
     5881 tgccaagtct cacggttacc ttttccgggc gaaaggtcac agaggtcgcc ccgatggctt
     5941 ggacgatccg accccaattg ggatctccgc catggaccgc cgtcttgacc aacgggctga
     6001 cggccaccgc ccgtgccacc gcatgggcat ctgcgggaga ctttgcgccc cggacgatca
     6061 cctccagcac cttggtggct ccttctccat cgcggacgat ctggtgggcg agcgactcgc
     6121 agacggctct cagggccttg gaaaatgagg ccgtttgtcg ggtgttgaga tcgccggccg
     6181 ccccgctggc catgatggcc accgtgtcgc tggtggactg gcactcatcc acagtgaccc
     6241 ggttgaaggt tggtgcaacc gcctctttca gcagtctctt gagcactgcc ggcggcaccc
     6301 cggcatcagt cgtcaggaaa gccagcatgg tcgccatatt cggggcgatc attccgctgc
     6361 ccttgcagca accggcaacg gtgatcaggg tccgcccgat gcgcacccgc tcgcaggcct
     6421 gcttcatccg ggtatccgtg gtcatgatcg cctgggcaaa ccgctcaccg gcctcggccg
     6481 atgccgacaa ggtctcaagg gcggcatcga tccccggaag gatcttgtcc atgggaagaa
     6541 atcggcctat cacccccgtg ctcgacggca agacctcaat cggtttggca ccgatgcttt
     6601 cggcgagccg ccgacacatg gctcgggcat ctgcgatgcc gcgtttgccc gtggccacgt
     6661 tcgagcagcc gctgttaacc acgagagccc gcagacggcc ccggcgggtg tgttcccggc
     6721 cgacaatgac aggggccccg cagaagcggt tcgaggtaaa catggcggct gccgggcaga
     6781 tgtcatcggc gaccagtacc gcgatgtcga gcttgttgac ctgtttgatc atgcacaacg
     6841 acaccatcac cgctccggc
//
LOCUS       DAOTPW010000284         6841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9630_length_6841_cov_5.095491, whole genome shotgun sequence.
ACCESSION   DAOTPW010000284 DAOTPW010000000
VERSION     DAOTPW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9630_length_6841_cov_5.095491"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="P5316_19105"
     CDS             <1..1679
                     /locus_tag="P5316_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87124.1"
                     /translation="KARPIFEQCSANVAALKDELSQAKVLPERLNVQAAVAVAAEMLS
                     TAGGDSGTRRELIIVSDFQRSNWASVDFSPLPSDTVIELEATGPAEAPPNLAILRAGV
                     QGRPERNREARMEVEIGNYSTSPRRVTVDLQIGEATYRLEGLCPPGISTTLSGPVTLR
                     GSGWQTGRAVLRGIDDALPADDRRDFVLDVRPRPTYALVSRQRPDDRPSSSYYLERGL
                     APLEPKLGQEVERIVRIPPDQLSRETLASAELIALDHPGPLSVETAGLLAAMMRRGRA
                     VLYVAAEPVDASNLKRIVDAAGSDLQMPVEFVPPGPGQVRKNLFLTEVREKDAPFSVF
                     GDQVAAAIGPLRFAGGLASRRTESALAEDLLATYSDRTACLVTTACGAGHLTVLNADL
                     SESNLPRSPAFVPLIGELVSRMLGQDRAVNAVPCGEPLAMYLPPDSGTAAGLQILGPE
                     GAGDFGELATEGTWVVWRWPAAGYPGVYQVQRDDKTVFAVATGIAAQESDLTSLSGSV
                     FKDRLAGGRTVHYRSAAAEQDKQDDIWLWFAVACVACLCVELGVLRVFRT"
     gene            1707..4028
                     /locus_tag="P5316_19110"
     CDS             1707..4028
                     /locus_tag="P5316_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRR87125.1"
                     /translation="MAELTFEPLITPTLWLTLAVLATALMVIYASRRPASIKQGRWAV
                     VITLMSTGLLLTLLILLNPTWTNKIEPPAGKPLLTVLVDTSASMATTDANESRTRYDV
                     ARVTAQEAARDLRRFFDVRVRTFDSLAAPAEPEELSGRSPGGGMTDLASAITGSLEQD
                     RPQGQAMLLLSDGIHNAPGGVDGVRTAAQFAKAMSAPVYTKTIGGQASVYDLRVEVYS
                     PQQLAYIGQKVPIRAVVKHVGLPGAMAKVSLLHDEKTIDQRETRLMPDGPTDVRFFVS
                     KQKRGLYRYEVKVEPLPGEVVQVNNVASCVLQVVDEPIRVLLLEGKPYWDGKFLMRTL
                     AADPAIAIESIVRLTDGRLLRRMLSRAASQPAAGNATETPAAVTGGNATQPAGGRPES
                     WTILTRAADTLADPEKIKVYQIIVLGRDTERFLDGAAIANLRNWISREGGSLVCYRGA
                     PVAQVDERLERLLPVRWSPTRETRFRMQLTEQGRDLRWLPTVGNLTEGESLPQLPTLA
                     TVARPEKPKPMAVVLAAGAAEGGSEPAPVLTYQAYGMGRVVVVEGAGMWRWAFLPPEH
                     QVHDEIYAALWQSLLRWLVSNEHLRPGQDCTLQADKITFSATERASATLLMREEAARG
                     RIPSVELSSTALNRSETFAPIATGDNPGVFRVDFGRLPEGRYEARIQGQKKDQDDQSG
                     RIAFDVRNFAEERLDLQARPDLMARIASDSGGAVIESDAASEIHHGFQTYLARSRPAR
                     ITRTTAWDRWWVFTAIMLVWGAAWGLRRSGGLV"
     gene            4025..6820
                     /locus_tag="P5316_19115"
     CDS             4025..6820
                     /locus_tag="P5316_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87126.1"
                     /translation="MTSTFLTDCLRRVRKRTLLVGLSAGVGWALLVWLVFLAISMWVD
                     LVLELPPSLRLTSTCVAMAFGLGWAAWAAWSVVRRSRPGDLARKMDAVARTGGQILSG
                     VDLLLDSRPRKGLARGLAEMAVDRAARLAGRVSTTSVVPARPVNRAFGVLGGALLATG
                     ALGLALPGLVGTQWARFFDPFDDHPPYSRVTFSVDPGSATVVYGDPLEIHARTRGPIV
                     DRLELVLSTNGGTSEETLPMFPENDGSWRTSIAHVTDAAQYFIRAHRARSKRYSIGVI
                     TVPRLDQARFRVTPPAYTHLKPYEGPLPQGGLAGLPGTRVEVWLKSNRPLSGGDVQLE
                     TQQEPGQVTLQPTVPGGNEATGSFEIRRPGKVSMGVTDIEGQASRDRLTAAITVLSDQ
                     RPFVRLLEPQAISLATPDAVLPVVMAAEDDYGISRLQLFRNLNESRALPQDIEVPPPA
                     PTRRGETTELALSQCKLEPGDEIKLYARVEDTDPAGVKGSESAISVVRIISQADYEKM
                     MLMREGLEMMQSKYQEAQRRMEAITDEIDKLKKELEKEPPDSELSREMQRRLEELTKR
                     MGDEADEIREAAGHKLPYDLDKYLTPELMKLAKSLDEAARELEQLRKKETQDGKGGFP
                     SVKGPLDKLKELSECLGGRKQDFEERATEPIEHLAKIFPLLEDEARYLNLYARQRDLA
                     ERLASLKGRDNEDDPELKARMRDLEAEQQRLREDLRELLDDIEDHVAQLPEDERLDEF
                     REMASKFAKDVRASGAAEAMAEAEEGLAAFSGTRGHAGAQEAADILDQFISRCNSMGN
                     GGKSCLKFSPTLASGLGNSIEQLLESMGMSMGSSGVGPAGASGYSASRNSLQNVGLYG
                     QLPIMSQTTAGGQGKDRMAALPSRQTGSGADEHNPWAVDPGGKLQAAGAASAIVPVTY
                     RSRVGSYFQRVADETGK"
BASE COUNT         1287 a         2060 c         2323 g         1171 t
ORIGIN      
        1 cgaaagcacg cccgatcttc gagcaatgct cggccaatgt cgccgcgttg aaggatgaat
       61 tgtcgcaagc caaggtgtta ccggaacggt tgaacgtcca ggcggcggtt gcggtcgcgg
      121 ctgagatgct gagcacggcc ggcggcgaca gcggcacgcg gcgcgaactg atcatcgtca
      181 gcgattttca gcggtcgaac tgggcaagcg tcgacttctc gccgctgccg agcgacacgg
      241 tgatcgaatt ggaggccacc ggcccggccg aggcgccgcc caacctggcc atactccggg
      301 ccggtgtaca aggacgaccc gaacgcaacc gcgaagcccg aatggaggtg gaaatcggca
      361 actactccac ttcaccccgg cgggtgaccg tggacctgca gatcggtgag gccacctacc
      421 gactggaagg gctttgcccg ccgggcatct cgacgacgct atccggcccg gtaacgcttc
      481 gcggctcagg ctggcagaca ggccgtgcgg tgctgcgggg tattgatgac gcattgcccg
      541 cggacgaccg tcgtgacttt gtactcgacg tgcgtccgcg accaacttac gcacttgtca
      601 gccggcagag gccggatgat cgcccatcat caagttacta cctcgaacgg ggactggctc
      661 cgctggaacc caagcttggg caagaggtgg aaaggattgt gcggatacca ccggatcaac
      721 tgagccggga gactctggcg tcggcagaac tgatcgcctt ggatcatccg gggccgctat
      781 cggtggaaac ggcaggccta ctggcggcga tgatgcggcg gggtcgagcg gttctgtatg
      841 tggcggcaga accggtggac gcgagcaacc tcaagcggat cgtggacgcc gccggttcgg
      901 atctgcaaat gccggtcgag tttgttccac cggggcccgg gcaggtccgg aagaacctct
      961 ttctgaccga ggtccgggag aaagacgcac ccttcagtgt gttcggcgat caggtagccg
     1021 ctgcgatcgg tccgttgcgt ttcgcgggag ggctggcatc gcgccgcacg gagtcagcgc
     1081 tggctgagga tctgctggcg acctacagcg atcggacggc gtgcctggtg accaccgcgt
     1141 gtggagcggg ccacctgacc gtcctgaatg cggacctgag cgagtcgaat ctcccacgat
     1201 cgccggcttt cgtgccgctt attggcgagt tggtcagccg catgctgggc caggatcggg
     1261 ccgtgaacgc cgttccttgc ggtgagccgc tggccatgta cctgccgccc gacagcggca
     1321 cggccgcggg actccagatc ctcggcccgg agggtgcggg cgatttcggc gagctggcca
     1381 ccgaaggtac gtgggtcgtc tggcgatggc cggcggccgg gtacccgggc gtctatcagg
     1441 tccaacgcga cgacaagacc gtttttgccg tcgccaccgg catcgccgcg caggaatccg
     1501 acctgacaag cctgagtggc tctgtgttca aagaccggct tgcgggcggc agaacggtcc
     1561 attaccgcag cgccgcagcc gagcaggaca agcaggatga tatctggttg tggttcgcag
     1621 tggcgtgcgt ggcgtgtttg tgtgttgagt tgggtgtgct gcgagtgttc cgaacttgag
     1681 gtgtcgggca cattgccggg cttcacatgg ctgagctgac atttgagccg ctgatcaccc
     1741 ccaccttgtg gttgacgctt gccgtcctgg caactgcgct aatggtcatt tatgcgtcgc
     1801 gtcgacccgc ctcgatcaaa caaggccggt gggccgtggt catcactctg atgtcgaccg
     1861 ggctgctgct caccctgctg atcctgctga atccgacctg gaccaacaag atcgagcctc
     1921 cggcgggcaa accgttgctg acggtactcg ttgacacatc cgccagcatg gcaaccaccg
     1981 acgcgaatga gagccggact cgctatgatg tcgcacgagt gactgcccag gaagcggcgc
     2041 gcgatcttcg acgtttcttc gatgtgcgcg tgcggacgtt tgactctctg gccgcccccg
     2101 ccgagccgga agaactgagc ggccgcagcc ccggcggggg aatgaccgat ctggcctccg
     2161 cgatcaccgg cagtcttgag caggaccggc cccaagggca ggcgatgctc ctgctcagcg
     2221 acggcataca caatgcgccg ggaggcgtcg acggcgtccg gaccgcggca cagttcgcca
     2281 aggcgatgtc cgcgcctgtt tacacgaaga ccatcggcgg ccaagccagc gtgtacgatc
     2341 tccgcgtgga agtgtattcg ccgcagcaac tcgcctatat cggtcagaag gtgccgatcc
     2401 gggcggtggt caagcatgtc ggcctgcccg gagcaatggc caaggtctcc ttgctgcacg
     2461 acgagaaaac aatcgaccaa cgggaaacgc ggctgatgcc cgacggccct accgacgtcc
     2521 gtttcttcgt cagcaagcag aagcgaggtc tctaccgtta cgaagtaaag gtcgaacctt
     2581 tgccgggtga agttgtgcag gtcaacaacg tcgcctcctg cgttctccag gtggtcgatg
     2641 agccgatccg cgtcctgctt cttgaaggaa aaccatactg ggacggcaag tttctgatgc
     2701 gaacgctcgc tgcagacccg gcgatcgcaa tcgagagcat cgtgcggctg accgacggcc
     2761 gcctgctgcg tcgcatgctg agccgggcgg cgtcgcaacc cgcggcgggc aacgccaccg
     2821 agacccctgc ggcggtcacc ggcggcaacg cgacgcagcc ggccggcgga aggccggaaa
     2881 gctggacgat cctgacccgt gcggccgaca cgcttgccga tccggaaaag atcaaggtct
     2941 accagatcat cgtcctcggc cgcgataccg agcgcttcct cgatggagca gcgattgcta
     3001 acctccgcaa ctggatctcc cgcgagggag gctcgctggt ctgctaccgt ggggcgccgg
     3061 tggcccaagt cgacgagcga ctcgagcggt tactcccggt tcgctggtcg ccgacgcggg
     3121 aaacacgttt ccggatgcag cttaccgaac agggtcgcga cctgcgctgg ctgccgacgg
     3181 tgggcaactt gaccgaaggc gagtctctgc cgcaattgcc gacgctggca acggtcgccc
     3241 gaccggagaa gccaaagccg atggccgtgg ttctggccgc cggcgcagcc gaaggcggaa
     3301 gtgagccggc ccccgtgctg acctaccaag cctacgggat ggggcgggtg gtcgttgtcg
     3361 agggcgcggg catgtggcga tgggcgtttt tgccgccgga gcatcaggtg cacgacgaga
     3421 tctacgccgc gctgtggcag agcctgctca gatggctggt ctccaacgag catctgcggc
     3481 ccgggcaaga ttgcaccctg caggccgaca agatcacctt cagcgcgacc gagcgggcga
     3541 gtgccacgct gctgatgcgc gaagaagcgg ctcgcggtcg aattccgtcg gtggagttga
     3601 gcagtaccgc gctcaatcga tcggagactt ttgcaccgat cgccacgggc gacaatccgg
     3661 gtgtcttccg ggtggatttc ggccggctac ccgaagggcg gtacgaggcg aggatccagg
     3721 ggcagaagaa agatcaagat gaccaaagcg gccggatcgc ttttgacgtc aggaacttcg
     3781 ccgaggagcg gctggatctg caggcccggc cggacctgat ggctcggatc gccagtgaca
     3841 gcggcggggc ggtgatcgaa tcagacgccg cctccgagat tcatcacggc ttccagacct
     3901 acctggcgcg cagccggccc gcccgcatca cccgcacgac cgcctgggat cgttggtggg
     3961 tgtttacagc catcatgttg gtctggggcg cagcctgggg ccttcgccga tcgggaggcc
     4021 tggtatgaca agcacgtttc tgaccgattg tcttcggagg gttcgcaagc ggacgctgct
     4081 tgtcggcctc tcggccggag tcgggtgggc gctgctggta tggctggtct tcctggcgat
     4141 cagcatgtgg gtcgatctgg tgcttgagct gccgccctcc ctgcggttga cgtccacctg
     4201 cgtcgcgatg gcgttcggac tgggctgggc ggcgtgggcc gcatggtccg tggttcgtcg
     4261 aagccggccg ggcgatctgg cacggaagat ggacgccgtc gcgaggaccg gcgggcagat
     4321 cctgtcgggc gtcgatctgc tcttggactc gcgcccgcgg aagggccttg cgcgagggct
     4381 ggccgagatg gcggtcgatc gggcggcgcg gcttgcggga cgcgtttcga cgaccagcgt
     4441 ggtaccggcc cgtccggtga atcgggcgtt cggagtgctg ggcggcgcct tactggcaac
     4501 cggcgcgctg ggacttgcgc ttccggggct ggtcggcaca caatgggcga ggttttttga
     4561 cccctttgac gatcatcccc cctactcacg cgtcaccttc agtgtcgatc cgggttcggc
     4621 aaccgtcgtc tacggtgacc cgctggagat ccacgcccgg acccgcggac cgatcgtcga
     4681 tcgcctcgaa ctggtgttgt ccacgaacgg cggcacttcc gaggaaaccc tgcccatgtt
     4741 ccccgagaat gacgggagtt ggcgaacgag cattgcgcac gttacggacg ccgcccaata
     4801 cttcattcgt gctcaccgcg cccgcagtaa gcgatacagt atcggcgtca ttaccgtgcc
     4861 gcggctcgat caggcgaggt ttcgcgtgac gccgccggct tacacgcatc tcaagccgta
     4921 tgagggaccg cttccgcagg gcggactggc gggcttgccc ggaacacgtg tggaggtctg
     4981 gctcaagagc aatcggccgc tgagcggcgg ggatgtgcag cttgaaacgc agcaggagcc
     5041 cggccaagtg acgcttcaac cgacggtgcc gggcggcaac gaagccacag gctcatttga
     5101 gatccgccga cccggcaaag tgagcatggg cgtgacggac attgaaggcc aggcttctcg
     5161 ggacaggctg accgcggcga tcaccgtgct gagcgaccaa aggccctttg tgaggctgtt
     5221 ggaaccgcag gccatatcgc tggcgactcc tgacgccgta ttgccggtcg tcatggcggc
     5281 ggaggacgat tatggcattt cgcggctgca attgttccgc aatctcaacg agtcgcgcgc
     5341 ccttccacag gacatcgagg tgccgccccc ggctccgacg cgtcgcggcg aaacgacgga
     5401 attggcgctc tcgcaatgca agctcgaacc cggagacgaa atcaagctct atgcccgtgt
     5461 cgaggacacc gacccggccg gcgtcaaagg atccgaaagc gccatttcgg tcgtgcgaat
     5521 catctcgcag gcggactatg agaagatgat gctcatgcgc gaaggcctgg agatgatgca
     5581 gtccaagtac caggaagccc agcgacggat ggaggcgatt acggacgaga tcgacaagct
     5641 gaaaaaggaa ctggagaagg aaccgccgga cagcgaactg agtcgggaga tgcagcgcag
     5701 gctggaggaa ttgaccaagc gtatggggga tgaggccgac gagatccggg aggcggccgg
     5761 acacaagctg ccctacgatc tcgacaagta cctgaccccc gaattgatga aactcgccaa
     5821 gtcattggat gaggccgccc gggaactcga acaactcagg aaaaaagaga cacaggacgg
     5881 caaggggggg ttccccagcg ttaagggtcc tttggacaag ctgaaggaac tgagcgagtg
     5941 tctcggcgga aggaaacaag acttcgagga acgggccacg gaaccaatcg agcatctggc
     6001 taagatcttt ccactgctgg aggatgaggc ccggtacctc aacctctatg ctcgacagcg
     6061 tgacctggcc gagcgtctgg cttcgctcaa aggccgcgac aacgaggacg accccgaatt
     6121 gaaggcccgg atgcgggatt tggaagctga gcagcagcga ctgcgggaag acctccgtga
     6181 actactggat gatatcgagg accacgtcgc ccagttgcct gaagacgagc gcctggacga
     6241 gttccgcgaa atggcgtcga agttcgccaa ggacgtgcgg gcaagcgggg cggccgaggc
     6301 gatggccgag gctgaagaag gattggcggc attctcgggc actcgcggcc acgcgggtgc
     6361 gcaagaagcc gcggatattc tcgaccagtt catcagtcgg tgcaacagta tgggtaatgg
     6421 cggaaagagt tgcctcaagt tctcgccgac gttggcctcc ggcctgggca acagcatcga
     6481 gcaactgctg gaaagcatgg gcatgagcat gggttcatca ggcgtgggtc cggccggagc
     6541 aagcggctac agcgccagcc gcaattcgct gcaaaatgtc ggactctacg gccagttgcc
     6601 gatcatgagc cagacgaccg ccggcgggca gggcaaggat cggatggccg cccttccctc
     6661 ccgacagacc ggctccggcg ccgacgagca caatccgtgg gccgtcgacc ctggcggcaa
     6721 gcttcaggcg gctggggcgg ctagtgccat tgtaccggtc acttatcgca gtcgcgtggg
     6781 atcgtatttc cagcgcgtgg ccgatgagac aggcaaatag ggttcagggt tcagggttca
     6841 g
//
LOCUS       DAOTPW010000285         6825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9661_length_6825_cov_5.329542, whole genome shotgun sequence.
ACCESSION   DAOTPW010000285 DAOTPW010000000
VERSION     DAOTPW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9661_length_6825_cov_5.329542"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1526
                     /locus_tag="P5316_19120"
     CDS             66..1526
                     /locus_tag="P5316_19120"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR87127.1"
                     /translation="MAGSILLVDADPQIRQLVESACSDLSVALRVVETLDEAFQTIEA
                     SPPDVTLLDMGSPEGAGLTMLVQLRARWPRLCAVLIAERARSDQAIEAIKHGAIDYLL
                     KPLRREDLIHHLSNALRISHDMIVPAVYESSDRDQPVDRIVGQSPAIHEVFKLIGLIA
                     PREVNVLITGESGTGKELVARAIYQHSRRKDRPFLAVNCAAIPETLLESELFGNEKGA
                     YTGADVRRIGKFEQCNSGTLLLDEIGDLPLSTQAKLLRVLQDGTFQRLGGNETIHCDV
                     RILASTNQPIEQMIAERRFRQDLFYRLKVASIHLPPLRDRDVDAVLLAHYFVRRFNPQ
                     IGSNVQKFSPEVISAFLNYPWPGNVRELENVIKASLVVARGSVFQIGFLPEPIRACAR
                     AGAPAPLEPQPDAHTEAREPHLRELCARLLDHQQFHGQLYERVIQTAEREVIRACLAR
                     TNGQMAPAARLLGITRTTLRKKMAELNIKIRRSVSE"
     gene            1870..2295
                     /locus_tag="P5316_19125"
     CDS             1870..2295
                     /locus_tag="P5316_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HRR87128.1"
                     /translation="MTGYTTSADWRTDTPPSGIPVPARVTEGRGDSSLPLEILDELRQ
                     ILLERRASLSQQLAALEAEAAVCQSRLSSSPADPADRRRAAWECALSRTNINRKTSLL
                     WEVDQVLRRMDEGTYGFDVTTGAPIPIERLREIPWARHA"
     gene            2536..4539
                     /locus_tag="P5316_19130"
     CDS             2536..4539
                     /locus_tag="P5316_19130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HRR87129.1"
                     /translation="MVKHKKHSDGGQMLMAFASPANAGKGNGNRTTSPAAAQSAAKAA
                     DAHSTPRSTAKPGPKSGRDDTDDRKPARSRTTHATAESMAKMQRDISVSEFFAKNRHL
                     LGFDNKRKALLMCVKEAVDNALDACEEAGHLPEIEVRIAQTAEERFRVTVKDNGPGIV
                     KQQIPNVFGKLLYGSKFHRLKMSRGQQGIGISAAGMYGLLTTGKSVKITSRISPKKDA
                     HYYEIQIDTKKNAPNIVKDETIKVDWDTGTQVEIELAGSYNKGRQSVDEYIEQTAIAN
                     PHVRIHFRPPVGEPVTYDRGTDVMPAQTQEIKPHPHGVELGLLIKMLHDTKSHWLSGF
                     LQSEFCRVGPKVAEEICQAARLSPRAHPKRIARQEAEKLYEAIQKTKLMSPPTNCLAP
                     IGAEQILTGLLKGVKAEFFTASTRPPAVYRGNPFQIEVGLAYGGSLGPDPKAENEGDS
                     EVAVDALRAFRRGGKLKTGNGNGSGSQMLARVIRFANRVPLLYQQSACCMYKSVIDVD
                     WRRYGLSQSSGALPQAPMVVLVHMASVWVPFTSESKEAIADYDEIRAEIKLALQDCGR
                     KLSAYLNRRRKMKYQGERRSIFEKYITEVVNACATIKRINRTELCENLLAMSKKATAQ
                     ADDQLDKHGKRVGGDSEYGENTVVVEQQDEASGNPGPLFQATG"
     gene            4995..6155
                     /locus_tag="P5316_19135"
     CDS             4995..6155
                     /locus_tag="P5316_19135"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRR87130.1"
                     /translation="MARTKSTVPASGSGGRTSAAAKAKAKETARKIEELAEMIVENAR
                     RRKSPKLEIPIRALSNVNFNPKKAILEMGDASQTRQFFDLSMARKFMQTMLVAAQCKK
                     LIDEEKTSSIRGLFYLCKHDVAGSKEKTFNDQDESDPIIEDLEVTIDALREELHVYAS
                     NRGNLVGPITLVDDGNTLDCMRMGSAGYAVPSICEPDVIQFKKCSAKYILHVEKDTVW
                     RRFYEDRFHEKHNCIITHGSGQPTRGMRRLLRRMHDELKLPVYCLLDNDPWGYYIYSV
                     IKQGSINLAYESRRMAVPQAKFIGVSSFDYERCGLTDDVKIGLDDKDIKRAKQILEYP
                     WFQGKKQWEKEIRKMMQNGFKMEVEAMINKRLTYLTEEYVPMKIKDPKQWLD"
     gene            6249..6494
                     /locus_tag="P5316_19140"
     CDS             6249..6494
                     /locus_tag="P5316_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRR87131.1"
                     /translation="MLEAIRQCMSRAMKLDATTLASITEQTTAADVPGWTSVAHLSLI
                     LELERTFKITFDNDEIVSMGSVTAIIQGLKAKGVFGG"
     gene            complement(6641..>6825)
                     /locus_tag="P5316_19145"
     CDS             complement(6641..>6825)
                     /locus_tag="P5316_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87132.1"
                     /translation="PRRRARRPARTPDTQELPAKSRRYRRRMTTSEKVTLCRLPGRTP
                     IAATPFANPGVNAAIW"
BASE COUNT         1515 a         2184 c         1909 g         1217 t
ORIGIN      
        1 ggcgaaccga acactccgcg acatcacacc ttcaatccca ttcgcgctac cgttaggatg
       61 acgtcatggc tggaagcatt cttctcgttg acgccgaccc ccaaatccgg cagctggtcg
      121 aatcggcctg ctccgacctc tcagtcgccc tgagggtcgt cgaaacgctc gacgaggctt
      181 tccagaccat cgaggcgtcg ccccccgacg tcaccctcct tgatatgggt tcccccgaag
      241 gagccggcct tacgatgctc gtgcagcttc gagcccgatg gccaaggctt tgtgccgtcc
      301 tgatcgccga gcgcgcccgc agcgaccagg ccattgaggc catcaaacac ggggccatcg
      361 attatctcct caagccgctt cgccgcgagg atctcatcca tcacctcagc aacgccctgc
      421 ggatcagcca cgacatgatc gtccctgcgg tttacgaaag ctccgatcgc gaccagcctg
      481 ttgaccgaat cgtgggacag tctccggcga tccacgaagt cttcaaactc attggcctga
      541 ttgcccctcg tgaagtgaac gtactcatca ccggcgaaag cggcaccgga aaggaactcg
      601 tcgcccgggc catctatcag cacagccggc gaaaagatcg gcccttcctc gccgtcaact
      661 gcgccgccat ccccgaaacg ctgctcgaga gcgagctctt cggaaacgaa aaaggcgcct
      721 acaccggcgc cgacgtccgc cgcatcggca agttcgagca gtgcaactcc ggcaccctcc
      781 tgctcgacga aatcggcgat ctgcccctct ccacccaggc caagctgctt cgagtccttc
      841 aggatggcac cttccagcga cttggcggga acgaaacgat ccactgcgac gtgcgaatcc
      901 tcgcctctac aaaccaaccc atcgagcaaa tgatcgccga acgacgcttc cgccaggacc
      961 ttttttatcg cctcaaagtc gcttccatcc acctgccccc tctgcgggat cgcgacgtcg
     1021 acgccgtcct gctggcccac tatttcgttc gtcgattcaa cccccagatc ggctcgaacg
     1081 tgcagaagtt ctcccccgag gtgatctccg cgttcctgaa ctacccctgg ccgggcaacg
     1141 tccgcgagct ggagaatgtg atcaaggcct ccctcgttgt cgcccgcggt tccgtgttcc
     1201 agatcgggtt cttgccggag cccattcgcg cctgtgcccg ggctggagca cccgcgcccc
     1261 tcgagcccca gcccgacgcc cacactgagg cccgcgaacc ccatcttcga gaactgtgtg
     1321 cccggttact cgatcatcag cagttccatg gtcagctata tgagcgagta attcagaccg
     1381 ccgagcggga ggtcatccgc gcctgcctgg cacgcaccaa cggccaaatg gccccagccg
     1441 caaggctcct gggaatcacc cgaaccaccc tccgaaaaaa aatggcagag ctcaacatca
     1501 agatccgcag gtcagtctca gaatgaataa ataacgatct tcgtggacgt ttagcgatct
     1561 acgtggctat ttactagcca gatatggccg acactgtctc gcctcccgtt ttcttaaact
     1621 attgtttcaa aatcacttac agaaacaagt gaggcttgga ccattggcac ggcagatgcc
     1681 agatctggaa cctgcggaga gcctgtctgt tcgacaaccc tcgtgcgcgg ctttggggag
     1741 tcaactgtcc acaagggttg tcgaactgtt ggcggattcc gggatcagga tggcttcatg
     1801 gaccggctcc ggttaccgct ggcacgacgg ccccggggag ggcagcatca gccgaaggag
     1861 gacttgccaa tgacagggta tacaacatct gcggattgga gaaccgatac gcctccctcc
     1921 ggcattcctg tgcccgctcg cgtcactgaa gggcgcggtg attcttcatt gccattggag
     1981 atcttggatg aattgcgcca gattctcctt gaacgccgcg cgtccctctc ccaacagctt
     2041 gctgcactcg aagcagaggc agccgtctgc caatcccgcc tgtcaagcag cccggcagac
     2101 cccgcagaca ggcgacgggc cgcctgggag tgcgctctct cccgcaccaa catcaaccgc
     2161 aaaacgagcc ttctgtggga agtcgaccag gtgcttcggc gcatggacga aggaacctac
     2221 ggtttcgacg tgacgacggg agcccccatc ccgatcgaac ggctccgtga gattccgtgg
     2281 gcaagacacg cgtgaatacc ccagccgaga acggcttgat ccccattccc cctgagttga
     2341 aactcttcgc aacgcccgcc gactacactg tgggtgttct tcttgccccc caacagccga
     2401 tgtacacttc agaacgacgt ggcggcaggc tcggcacgcc cgtaggtggt tgcatcaaca
     2461 gcccggcgtc atatcatggt gccggatggg agagcttgtg gagagaattg accctgagcc
     2521 agggacggct tccctatggt gaagcacaaa aagcattcgg acggcggaca gatgctcatg
     2581 gcgtttgcgt cgcccgcaaa cgccggtaaa ggcaacggga acaggacgac atctcctgct
     2641 gcggcccaat cagccgccaa ggcagccgac gcgcattcga caccgcgatc gaccgccaag
     2701 cccggcccga agtccggccg cgacgacacc gatgacagaa agcccgcccg ttccaggaca
     2761 acacacgcca cggccgagtc catggccaag atgcagcgag acatctccgt ctccgagttc
     2821 ttcgccaaaa accggcacct cttgggtttc gacaacaagc gcaaggccct gctgatgtgc
     2881 gtcaaggaag ccgtcgacaa cgccctcgat gcatgcgagg aggccggcca tctgccggaa
     2941 atcgaagtcc ggatcgcaca gaccgccgaa gaacgcttcc gggtgaccgt caaggacaat
     3001 ggccccggca tcgtcaaaca gcaaatcccc aacgtgtttg gaaagctgct ctacgggtcc
     3061 aagttccatc gactcaagat gtcccgcggc caacagggca taggcatctc cgcggccggc
     3121 atgtacggcc tgctcaccac cggcaagagc gtcaagatca cctcgcggat ttcaccgaag
     3181 aaagacgctc actactacga gatccagatc gacacaaaga agaacgcgcc caacatcgtc
     3241 aaggatgaaa ccatcaaggt cgactgggac accggtactc aggtcgagat cgaactcgcc
     3301 ggttcataca acaaaggccg gcagtcggtc gatgagtaca tcgagcaaac cgccatcgcc
     3361 aatccgcacg tccggatcca cttccgcccg ccggtcggcg aacccgtcac ttacgaccgc
     3421 ggcaccgacg tcatgcccgc gcagacccag gagatcaaac cccacccaca tggcgtcgaa
     3481 ctcggactgc tgatcaaaat gcttcacgat accaagtctc actggctcag cggttttctt
     3541 cagagcgaat tctgccgcgt cgggccgaag gttgccgagg agatctgcca ggccgccagg
     3601 ctcagcccca gggcccaccc gaagcgcatc gcccgccagg aagccgagaa gctttacgag
     3661 gcaatccaga aaaccaagct gatgtcgccg ccgaccaact gcctggcacc catcggtgcc
     3721 gagcagatcc tgaccggcct gctcaaaggc gtgaaagccg agttcttcac cgcctcgacg
     3781 cgcccccctg ccgtctatcg cggaaacccc ttccaaatcg aggtgggact ggcctacggc
     3841 ggctcgctgg gacccgaccc gaaggccgaa aatgaaggcg actccgaagt ggccgtcgat
     3901 gccctgaggg ccttcagacg cggcggtaag ctcaagaccg gcaacggcaa cggctccggc
     3961 tcgcagatgc tggcccgggt gatccgcttc gccaaccgcg tcccgttgct ctaccaacag
     4021 tcggcttgct gcatgtacaa gagcgttatt gacgtcgatt ggcgccgtta cggcttgtcc
     4081 cagagcagcg gggccttgcc gcaggccccg atggtggtgc tcgtccatat ggccagcgtg
     4141 tgggttccgt ttacctcgga gtccaaggag gccatcgccg attacgatga gatacgcgcc
     4201 gagatcaagc tggccctgca ggactgcggc cgaaaactgt ccgcctatct caaccgccgg
     4261 cgcaaaatga agtaccaggg cgagagacga agcatcttcg agaagtacat caccgaggtg
     4321 gtcaacgctt gcgcaaccat caaacgaatc aacaggaccg agttgtgcga gaacctcctg
     4381 gctatgtcga agaaggcgac tgcccaggcc gatgaccagc tcgacaaaca tggcaagcgc
     4441 gtcggcgggg attccgagta cggcgagaat accgtggtgg tcgagcagca ggacgaggca
     4501 tcgggcaatc ccggtcccct tttccaggcg acggggtgag ccggctgccg ggatctctcc
     4561 ggcccacgcc aacgggagac gcgacgcgtc gcccgtcagc ctcaagctcc gcaaagccgc
     4621 tggtcatggt ttgtggcacc agaaaccgct ggaatcgtgg cgtatgaaga ttgctgatgt
     4681 gaccacctcg atgactcccg tgtgttcctc atgggaggtt gagatgaagg cccaacgagc
     4741 aggggcgaac gccccggaaa ggcttcgtcg gaccgttcag ccgctgctcg cggccgaaat
     4801 accttttatc gtgatgggtg gcaacgccgt ggccgcaagg gatgaaggtg cagtccccaa
     4861 caccagccat gccggcttga tggacgatgc tcctgcagaa gcagattcct tcggagcttc
     4921 ggccccgcct gcagaagatc cgcgtcaacc caaatggacg agtgaagaga cgtcgacgca
     4981 cggaggatgt ttgattggcc agaactaaat caactgttcc ggcgagcggc tccggaggca
     5041 gaaccagtgc cgccgcaaaa gccaaagcga aggaaacggc ccgcaagatc gaagagcttg
     5101 cggagatgat cgtcgagaac gccaggaggc gcaagagccc caagctcgaa atccccatcc
     5161 gggccctctc gaacgtcaac ttcaacccca agaaagccat ccttgagatg ggcgacgcct
     5221 cccagacccg ccagtttttc gacctgtcca tggccaggaa gttcatgcag accatgctgg
     5281 tggccgccca gtgcaagaag cttatcgacg aggaaaagac ttcctcaatt cgtggtctgt
     5341 tctacctctg caagcacgac gtggccggca gtaaggagaa aacctttaat gaccaggatg
     5401 agtccgatcc gatcattgag gatctcgagg tcactatcga cgcgcttcgc gaggaactgc
     5461 atgtctatgc cagcaaccgc ggcaacctgg tcgggccgat cacgcttgtg gacgacggca
     5521 atacgcttga ttgtatgcga atgggctcgg ccggttatgc cgtaccgagt atctgcgagc
     5581 ccgatgtgat ccagttcaag aagtgcagcg ccaagtacat cctccatgtc gaaaaagata
     5641 ccgtctggcg gcgcttctac gaggaccgct tccacgaaaa gcacaactgc atcataactc
     5701 acggcagcgg acagcccacc cgaggcatgc gacggctgct gcgacgcatg catgacgagc
     5761 tgaagctgcc cgtctactgc ctgctcgata acgacccctg gggatattac atctactccg
     5821 tgatcaagca gggctccatt aatctcgcct atgaaagccg acgcatggcc gtcccgcaag
     5881 ccaagttcat tggcgtgtcc agctttgact acgaacgctg cggcctcacg gacgacgtca
     5941 agatcggtct ggatgacaag gacatcaagc gggccaagca gatcctcgag tacccctggt
     6001 tccagggcaa gaagcagtgg gaaaaggaaa tccgcaagat gatgcagaac ggctttaaga
     6061 tggaagtcga agccatgatc aacaagcggc tcacgtatct caccgaagag tacgtgccaa
     6121 tgaagatcaa ggacccgaaa caatggttgg attgaagaga gagcggtcag cgatcagctt
     6181 tcagctctcg aaaatgtttc tggccgatgg ctgacggctg atcgctgaca acttctttgg
     6241 agttccccgt gctcgaagcg attcgtcaat gcatgtcccg tgccatgaag ctcgacgcga
     6301 cgacgctcgc gtccatcacc gagcagacca cggccgctga tgtccccgga tggacctccg
     6361 tggctcatct ctccctgatc ctggagctgg agaggacctt caaaatcacc ttcgacaacg
     6421 acgagatcgt ctctatgggc agcgtgacgg caatcatcca aggactcaag gccaaaggcg
     6481 ttttcggagg atgatacttc ctgcacgagc gcgctctgcg ggagaacggc acgacgtgtt
     6541 atcatgcccg caccgaccgg attcactctc cggcgacacc gacgatgact tcattgctct
     6601 ccctgcaatc ttttctcagt cccagtgctg cgtagggtgc ctaccagatc gcggcgttca
     6661 cgccgggatt ggcgaacggc gtggcagcaa ttggtgtcct tccgggaagc ctgcatagcg
     6721 tgactttttc cgacgtggtc atccgccgcc gataacggcg agactttgcc ggtagttcct
     6781 gggtatccgg tgttcgggct gggcggcgag ctctacggcg cggtg
//
LOCUS       DAOTPW010000286         6797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9706_length_6797_cov_5.833432, whole genome shotgun sequence.
ACCESSION   DAOTPW010000286 DAOTPW010000000
VERSION     DAOTPW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9706_length_6797_cov_5.833432"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(515..1960)
                     /locus_tag="P5316_19150"
     CDS             complement(515..1960)
                     /locus_tag="P5316_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87133.1"
                     /translation="MCHHCLNSCDGFSRREMLASAAGTAIIGGLLARQVQAAGAAASR
                     PATNREMPEVRAVFIRPKQDYWLGWPGTAWDKNMCGPFMNKCRQLVGQFAADMKIRLT
                     WADEPLHDDAATDKFVAAAKADNLDGTIVFPLHFQNWPQVAKIADSGIPTIIFAPLGV
                     CFTGHIQKIAKQAGVYLASCADFDLNPVRFGLRMVRAHHDIRRTRIAVLAGTQNKEQV
                     LEPLGLSLKFLPRERFVEALATVQVTPEVRAIADDYKKAAARIVEPTDEDLLNAARNY
                     VAARKILQEEDCQGITMDCLGLVAERKIPTPPCMAWCKFLDEGISATCEADIRPVMSQ
                     ELCLKLLGKPGFVQDPVPNTVDNTFIGAHCVCGTRIFGFDKDPAKFILRSHAESDLGV
                     SFQVIWPEGTDVTVMELMEPGRMILGQGKVIRNYETPPAGGCRTSVELELDGPADVRD
                     TQGFHQLFITGKHVRDFQAYAQMYGIVAEHI"
     gene            complement(2205..4202)
                     /locus_tag="P5316_19155"
     CDS             complement(2205..4202)
                     /locus_tag="P5316_19155"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRR87134.1"
                     /translation="MKRYLLANAFINLCVTIAAAAPAPSQPVSQEEALNWTRHLVPLP
                     KRIEFLSKVQVPVNQIGITLRDDKEPLAVQATRELHECLRAPQRPASDASIHLILKVD
                     PQESALASLPNRDQAYRIIPTEGSGLLLLGGGPRGLYYAAKTMQQLIKPGLTEDGAAI
                     PLVSITDWPDLEDRGLWGGDSSEQIRWMSDRKMNYDEQISTTGVDENKQCFVRYPPYK
                     QRMIDEGPIYGVNPVPVVLHLEQLAGGGLFKAYPELQGKDAKPGAICYSNPIFTDLLT
                     QWLLLWGEKPGVREVDVWMTENLGGQKGCQCDKCVKQDRNVLEARVIVAAWKKAREKQ
                     PNLGLRVLTSEETEKSNPKVFAELPPEVKVWYYHSLFTYNTSEKPMIRPYLVETARKG
                     RWIGVCANTCALVGCWQPFTGAHFMHYRMNEFVDKKLSGFLGYAVPRIRHVAFNTEAA
                     AEWGWNAKGRSPHEFALSWAVRQGMKDPQKFAEWSDTLGPVAWDVYGSEWPAGEKRKT
                     PGKVADMLKQGKLPDLGYVLWDVYPTPWGDIKTAEQLDSDVAHAERAVTLARELGEEV
                     LIQESLVVQGYINSLKALWILKQLVTSGGVADQDKESARRFFKMYLDSLDQAGKALTA
                     WEAALPGKGDREGIMRESVELVDGMIGEMAAVAAELGCSPR"
     gene            4552..6186
                     /locus_tag="P5316_19160"
     CDS             4552..6186
                     /locus_tag="P5316_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87135.1"
                     /translation="MHQYARVRSEIIFLVAVAGLCVFGGTATAQSAGQSPIRIGSQRQ
                     VFIDGTFLAEAKSIELVVHPPRKSGEMNIKPEHEWESGGIGPYSSVLHEGDTYHAWYH
                     AMDTVQWDSGHGRGSICYARSKDGIHWEKPVVGLIEYKGNRQNNIVMGHGANGTTLGQ
                     DGGMVFLDPNAPPDEKYRMAVRFGVKSEHGGMHLFSSGDGVHWKPTHPSVVYAVADDK
                     SHHLDSQNVIFWDDRIHKYVAYVRYNRPKEKGRARTIARGESDHLGGFPPVQQMQVVF
                     QPDENDLMEDGVPMVDFYMSAAVKYPWAANAYYMFPTAYYHYTGMLPEFTKECPANAG
                     PLDSQFAASRDGIKWERYDRRPFVPLGMRGEFDWGCARVIWGLVPDTTGREMFLYYRG
                     GDWLHGWDRDERNKRILTEKGLGASRNVAVISRLISRRDGFVSARAAYSGGEFVTPTL
                     IFEGNRLVVNIDTSATGTARVGILDPGGRAIDGFSVEECDLIHTCNEINRTVSWQGNS
                     DVHTLAGRPLKLRFVIRGCDLYAFQFENIARFGLSR"
BASE COUNT         1300 a         1947 c         2177 g         1373 t
ORIGIN      
        1 aaccaaccag acgaaccatc catggcccgt gacatccgta acaacatcgg caaagacgcc
       61 aaaaccggta caatgaatgt caacaggacc tagtgaggcc catgatggct gtcaagcccc
      121 cgaggggctg gcactacagc gcaaagtcgc tctcttgggc gggcggaccc gtgtttctga
      181 tagcgccagg gggcgtaacg aggcctgagc ttggttcaaa tcaaccacct tgcgctgtag
      241 tgctagtact cgctgacccg ctgagactgc tcagaaagcg catgcgtgcg cgatgccgcg
      301 tctggcgagc aaccgtgaac gacttcatgc cgaaccggtt caccaagctg atgaaccccg
      361 ttcccatcgt tcaatcgggg gttcaccccg gtgttgtata ccgcaacgat ggggaatgat
      421 gagaattgag aacggggcgt ggtatgtcgt taccgtagcc tggtacctgt gcggcggcgg
      481 cctgcgggtt tcagcttcgg gtgaagcctt ccgatcagat gtgctcggcc acgatgccgt
      541 acatctgggc gtaggcctgg aagtctcgca cgtgcttgcc ggtgatgaag agctgatgga
      601 aaccttgcgt gtcgcggacg tcggccgggc cgtcgagttc cagttcgacc gaggttcggc
      661 agccgccggc cggaggcgtt tcgtagttcc gaatcacctt gccctgcccc aggatcatac
      721 gtccgggctc catgagttcc atcacggtaa cgtccgtgcc ctccggccag atgacctgga
      781 acgagacgcc caggtcggat tccgcgtggc tgcgaagtat aaacttggcc gggtccttgt
      841 cgaaaccaaa gatccgagtc ccgcaaacgc agtgcgcgcc gatgaaggtg ttgtccaccg
      901 tgttgggaac cgggtcctgg acgaagcccg gtttgccaag gagcttgaga cacagttcct
      961 gagacatgac cggtcggatg tcggcctcgc aggtcgcgga gatgccttcg tcgagaaact
     1021 tgcaccaggc catgcatggg ggcgtcggga tcttccgttc ggcgaccagg ccaaggcagt
     1081 ccatggtgat accctggcaa tcttcttcct gaaggatctt ccgagcggcg acataattgc
     1141 gagcggcgtt gaggagatcc tcatccgtcg gctcgacgat cctggcagca gctttcttat
     1201 agtcgtcggc gatcgcccgc acctcggggg tcacctgaac ggtcgccagg gcttcgacga
     1261 atcgctcccg cggcagaaac ttcagcgaca agccgagcgg ttcgaggacc tgttccttgt
     1321 tctgtgtgcc agcgaggacc gcgatcctgg tccggcggat atcgtggtgc gcgcgaacca
     1381 tgcggaggcc gaagcggacg gggttgaggt cgaagtcggc gcaggaagcc aggtacacgc
     1441 ccgcctgttt ggcgatcttc tggatgtgac cggtgaaaca cacgcccagc ggggcgaaga
     1501 tgatggtcgg tattccggaa tcggcgatct tggccacctg gggccagttc tggaaatgca
     1561 gaggaaagac gatggtgcca tcgaggttgt cggccttggc cgcagcgacg aacttgtcag
     1621 ttgcggcatc gtcgtgtaag ggctcgtcgg cccaggtcaa acggatcttc atgtcggcag
     1681 cgaactggcc gaccaattgc ctgcacttgt tcatgaacgg gccgcacatg ttcttgtccc
     1741 aggccgtgcc gggccagccg agccaatagt cctgcttagg tcgaatgaaa accgcgcgga
     1801 cctcgggcat ttcccggttt gtcgccggtc gcgaggcggc cgcgccggca gcttggacct
     1861 ggcgcgcaag caggccgccg atgatggccg ttccggcggc ggacgcgagc atttcccgac
     1921 gtgagaaacc gtcacacgaa ttgaggcagt gatgacacat aggcgcgctc ctggttgtga
     1981 gaacacattc tgcacgtacc ttgctcggtg caggtcgtcg ccgcatgaat tggcccgacg
     2041 acgaatcccg gacggcatca tagccgatgg ccgtgaggcg tgacaaccgg cggtgacggg
     2101 ctgttcgcgc ggccggcgag gcgggcggca gacacccttt gcacggcatg caggttcaag
     2161 aagccgtcgc gccgacggag gagggactga ttcagaaccg gaggttaccg gggcgagcac
     2221 cccaactcag ccgccacggc ggccatttcg ccgatcatgc cgtcgaccag ttcgacggac
     2281 tctcgcataa tgccctcccg gtcgcctttg ccgggcaggg cggcctccca ggctgtcaag
     2341 gctttcccgg cctgatcaag gctgtccaga tacatcttga agaatcgcct ggcggactcc
     2401 ttgtcttgat cagcgacgcc gcctgatgtg acaagctgtt tcagtatcca gagagccttg
     2461 agcgagttga tgtatccctg cacgaccaga ctttcctgaa tgagcacctc ttcgcccaat
     2521 tcacgggcaa gcgtgaccgc tcgctcggcg tgggcgacgt cgctatcgag ctgctccgcg
     2581 gttttgatat cgccccaggg agtggggtac acgtcccaca agacgtagcc gagatccggc
     2641 agcttgccct gtttgagcat gtcggccacc ttgccgggcg tcttgcgctt ctcgccggcg
     2701 ggccattcgc tgccgtagac gtcccatgcg acgggcccga gtgtgtcgga ccattcggca
     2761 aacttctgcg ggtctttcat gccctgtcga accgcccacg agagggcaaa ctcgtgcgga
     2821 gaccggccct tggcgttcca gccccactcg gccgccgcct cggtgttgaa ggctacatgc
     2881 cggatccgcg ggacggcgta gccgaggaaa cccgagagct tcttgtcgac gaattcgttc
     2941 atgcggtaat gcatgaagtg tgctccggtg aagggctgcc agcaaccgac aagagcacaa
     3001 gtgtttgcgc acacaccgat ccagcggccc ttgcgagcgg tctcgaccag atacgggcga
     3061 atcatcggct tctcgctggt gttgtaggtg aacaggctgt ggtagtacca caccttgacc
     3121 tccggcggca gttcggcgaa caccttcggg ttgctttttt cggtttcctc gctggtcagg
     3181 acgcgcaggc caaggttcgg ctgcttttca cgcgcctttt tccaggccgc gacgatcacc
     3241 cgggcttcca ggacgttgcg gtcttgcttg acgcacttgt cgcactgaca gcccttttgt
     3301 ccgccgagat tctcggtcat ccagacgtcg acttcgcgca cgccgggctt ttctccccaa
     3361 agcagcagcc actgggtcag caggtccgtg aagatgggat tcgagtagca gatggcaccg
     3421 ggcttggcgt ccttgccctg aagctcggga tatgccttga agaggccgcc acccgcaagt
     3481 tgctcgaggt gcagcacgac cggaacaggg ttgacgccgt agatcggccc ctcatcgatc
     3541 attctttgtt tgtacggcgg ataacggacg aaacactgct tgttctcatc gacgccggtg
     3601 gttgagatct gctcgtcgta gttcattttc cgatccgaca tccagcggat ctgctccgag
     3661 ctgtcgccac cccacaatcc gcgatcttcc agatccggcc agtcggtgat gctgaccagc
     3721 ggaatcgcag cgccatcctc ggtcagcccg ggcttgatca attgctgcat cgtcttggca
     3781 gcgtagtaca gtcctctcgg cccgcctccg agcagcagca ggccgctgcc ttcggttggg
     3841 atgatgcgat aggcctggtc gcgattggga agactcgcca aagcggattc ctgggggtcg
     3901 accttcagga tcaagtggat tgaggcatcg gatgcggggc gctgtggcgc cctgagacac
     3961 tcatgcagct ccctggtcgc ctgaacagcc aaaggctcct tgtcgtcgcg cagggtaatc
     4021 ccgatctggt tcaccgggac ctgtaccttc gatagaaact cgattctctt gggcagtgga
     4081 accagatgcc gggtccagtt cagcgcttct tcctgagaca cgggctgaga cggggcggga
     4141 gccgccgctg caatggttac gcagagattg atgaacgcat ttgccaacag ataccgtttc
     4201 atgaaagact ccttcagcct gatcgattca agagccgtcg gcaggtctca tccgctgcgc
     4261 ggatttcgac catgtcctgt cttcattgca caccggcttc acgggattgt accgaaggtc
     4321 cttcactcaa gcgatagcac tctgactctg agatccgggc agggattcgg cacgaacggt
     4381 cccgatttgc gagcgtggtt tgtcgcgatc cgtcggcggg tgggggttga ttccggcgtc
     4441 aggggaaccg cgttagaata cccgcgccga gttgcgtggc aaggacttca cttgactctt
     4501 gttccgttgt ggcgggcaac gggacggcaa acgaaacgaa agggtgattc aatgcatcaa
     4561 tatgctcgcg tgcgaagcga aatcatattc ctggttgcgg tggccggatt gtgtgtattc
     4621 ggcggcacag ccacggcgca gtcagccggc cagagcccga ttcgcatcgg ttctcagcgg
     4681 caggtgttca tcgacggaac gtttcttgcc gaggccaaga gcattgagct cgtggttcat
     4741 ccgccccgca agagcgggga aatgaacatc aagcccgaac acgaatggga aagcggcggc
     4801 atcggcccgt acagcagcgt cctgcacgag ggcgacacct atcatgcgtg gtaccacgcc
     4861 atggataccg tccagtggga cagtggtcac gggcgaggtt ccatctgcta tgcccgatca
     4921 aaagatggga tccattggga aaagcccgtt gtcggtctga tcgagtacaa gggcaaccgc
     4981 cagaacaaca ttgtcatggg gcacggcgcg aacggcacca cgttggggca ggatggcggt
     5041 atggtctttc tggacccgaa tgctccgccg gatgagaaat accgcatggc ggtccggttc
     5101 ggggtcaaga gcgagcatgg ggggatgcac ctgttctcgt ccggcgacgg cgtccactgg
     5161 aagccgacgc acccttcggt ggtgtatgcc gtggcggatg acaagagcca tcacctggac
     5221 tcgcagaacg tcatcttctg ggacgatcgg attcacaagt acgtcgcata cgttcgttac
     5281 aaccggccga aggaaaaggg ccgtgctcga accattgccc ggggcgagtc cgatcatctc
     5341 ggcggttttc ctcccgtgca gcagatgcag gtggtcttcc agccggatga aaacgatctt
     5401 atggaagacg gcgtaccgat ggtggacttt tacatgagcg cggccgtcaa gtatccgtgg
     5461 gcggccaatg cctattacat gttcccaaca gcttactacc actacaccgg catgttgccg
     5521 gagtttacga aggaatgtcc agccaacgcc ggaccgctgg acagtcagtt tgccgctagc
     5581 cgggacggga tcaaatggga gcgatatgac cggcgtccgt ttgtgccgct ggggatgagg
     5641 ggcgagtttg actggggctg tgcgcgggtc atttggggtc tggtgccgga taccaccggc
     5701 cgggagatgt tcctctacta ccggggcgga gactggctgc acggctggga tcgagatgag
     5761 cgcaacaagc gtatcctgac cgaaaagggc ctcggggcga gtcggaacgt ggcggttatc
     5821 agccgtctca tctcccgtcg ggacggtttc gtttcggccc gggcggctta cagcggcggc
     5881 gaattcgtga cgccgacgct gatctttgag ggcaacaggc tggtggtcaa tatcgatacc
     5941 tcggctaccg ggacagcgag ggttggaatc cttgatcccg gcggccgggc gatcgacggg
     6001 ttctcggtcg aggagtgcga cctcatccac acctgcaacg agatcaaccg gaccgtctca
     6061 tggcagggca acagcgatgt tcatactttg gccggtaggc cgctgaagct gcggttcgtc
     6121 atacgcggct gcgacttgta cgcgttccag ttcgagaata tcgctaggtt tggcctctcc
     6181 cggtaagcgc cgtctcgtcc cgggtcaatc gagcgaggtt ccgaccccaa atttgcggga
     6241 cttggggcta gggaatccgg tcgggggcga gcggcgtctg atgggagctg aaggccgtaa
     6301 ccaaaacggc tgggcggagc cccaaatatg cgcgcatcag acggcggaag gggctaaagc
     6361 accggcataa ccctgggggc cacggattcc tgcatcatgt atacgagggt cgctaatgtg
     6421 tcagatcgaa tcagaaaacg tcggatttgt cgtttttctg attgacagac gtgttttcgc
     6481 ttgtttagaa taaagcacag ttagaatcac caatcgcggc tgactaggag gtgaggagtt
     6541 tggccgaagg tagtcatgcg aggggcgccg tcccggcgcg cgaatgggat tggttagagc
     6601 cgcttggtta cgcagggggg gaggtaggag ttccggtccc gaggttggtg tagccgcgga
     6661 gaaagtcaca gggtgtgggg agggcgatcg cgcctggcgg tgcgggatgt tactcgcagc
     6721 attcgcgtgg ggtttccatc aggtcgtcgc caggcaggat cgtcgattcg ccgcctgtgc
     6781 ggcaagatgc attgtct
//
LOCUS       DAOTPW010000287         6763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9766_length_6763_cov_4.690370, whole genome shotgun sequence.
ACCESSION   DAOTPW010000287 DAOTPW010000000
VERSION     DAOTPW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9766_length_6763_cov_4.690370"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="P5316_19165"
     CDS             <1..388
                     /locus_tag="P5316_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87136.1"
                     /translation="FRAASPAESFPHSAFMTMAIFHQQVGQIKALRSHTDESGRRHLP
                     GIRNRAPACLRHPAPTTQRHNRFGAAGKSRGLGTKLNPSRTRYGGNIIRVTATEVRAV
                     RIGNRTAADRYRKGRLKRADDAMVLE"
     gene            complement(428..1579)
                     /locus_tag="P5316_19170"
     CDS             complement(428..1579)
                     /locus_tag="P5316_19170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87137.1"
                     /translation="MHQKSDFSRRAVLEKGLGVASAVLLGAVRLRAEDTQPTTMPADD
                     LRCGMIGIGGRGSGLLNAVNKSPGVRVTALCDIDPKHLRAAAEKVQQDEPRLFEDYRK
                     LIEFKDLDAVVIATPVYLHAEMAVAVLSAGLHLYCEKPLGMTVKDCKAVLEAARSAKG
                     IYQGGTQLRYAHPWQSSIKLILSGEVGKPIFIRAHRHNVGDLPHDRQWLFEKRYCGDT
                     IVEQAVHEFDLFNWILGGPPVRAAGFGQQSLRFEPKGRDIMDNYALSLDYGKDKKVSY
                     SHSWISAPKIPMDGRQEIVYCEEAAVDVENGMVYPRNFGEPYKVTPAEPAGDSTQLAI
                     DDFFKCIREKRRPLADVQAGFDGAMAAILGREAMDKERVVTMEELLKNG"
     gene            1894..2454
                     /locus_tag="P5316_19175"
     CDS             1894..2454
                     /locus_tag="P5316_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87138.1"
                     /translation="MIPHRRSASSWGILAVTLLAFITQGCGQILNDNLFSSFGGNAVA
                     GLPRPNGAIIILVMNQTSSAAVVRVQVNKTRYVPGDDTTGDGTTGGGTTGDDTTIPAT
                     SLELSIPLEPYGPATELDHAMIVQECDVTSIQFLGGSIQSDTGGDPVEIPADLPPITY
                     GSQLFCGDVVAITIVGVGLQPVISIY"
     gene            2456..3055
                     /locus_tag="P5316_19180"
     CDS             2456..3055
                     /locus_tag="P5316_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87139.1"
                     /translation="MSQRFHVMKRLQTLRFATLVAATLAVLVSCTGCLNPELVNRING
                     RLYPIVPGNQPFLLVRVINNTNAMLDVPIVYNDGTADREFVITGLTPQVHETGVVLDW
                     PVMRLALCDLDNPYNPNIIATLPDGSISAVPFGYNALEAGVHYMSGDGIIFSLNGDVR
                     APAFVRVNIGRIDAQTQPGGFTRADPFEAANLILLLGGF"
     gene            3069..3842
                     /locus_tag="P5316_19185"
     CDS             3069..3842
                     /locus_tag="P5316_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87140.1"
                     /translation="MRRRKFLLFGLLTALVGLPIIIGASCPPPDDPAFVSIITGPGST
                     IGQQLPNCERGRVCISILNQTCTDTDVMLYVHDGYDLTGTYVTRRAVECCENQNATEP
                     CPCYRCGSNSGEMQLTPPELFQPQNRYEISTGQVIRTIPGRQDRLSALGGRITISIRC
                     EEVKTIGLEVGLEGELPGLIQERAGPDYRCTRVDLDCAQAYTEDVACGATIQYTIVDR
                     NNCANQNLTVFRIETNVSADCTQVETTDNSSGGGGMSGG"
     gene            complement(3995..4384)
                     /gene="rpsL"
                     /locus_tag="P5316_19190"
     CDS             complement(3995..4384)
                     /gene="rpsL"
                     /locus_tag="P5316_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRR87141.1"
                     /translation="MPTINQLIRRGRTKQARKSKVRDLDQCPQKRGVCLIVKTQTPKK
                     PNSALRKVARVRLTNGKEVTAYIPGVDHNLQEHSIVLIRGGRVRDLPGVRYHIIRGTL
                     DCAGVQNDKDGRPRRRSRSKYGVKKGK"
     gene            complement(4519..>6763)
                     /locus_tag="P5316_19195"
     CDS             complement(4519..>6763)
                     /locus_tag="P5316_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRR87142.1"
                     /translation="FYLNPIYLMTDSGARGSVDQIRQLAGMRALMAKPSGEIIETPIK
                     SNFREGLSVLAYFSSTHGARKGLADTALKTADSGYLTRKLADVAQNVIVTRLDCGTIN
                     GITKGAVYKGEEIAVPLRDSIIGRVARDTIRNPVTDQIIVAENDVITREIAAKIEELG
                     LDTIRVRSPLTCESPFGVCARCYGVDMSTGNLVEEGMAVGILAAQSIGEPGTQLTMRT
                     FHTGGVATRAVAENKIINIQAGRVVFHDLNAVEVPATEGEGTRQVALKRNGEVAIVDA
                     KNRELERYKVPYGALLVVREGQEVGPRTELATWDPHLTPILAEVSGVVRFHDITEGET
                     VRLEQERAGAKYIVVEHKGEKHPQIVIEDVEGRVLDYHYLPAKARIEVAEGDKIVPGT
                     LLARQPRAIAGTQDITGGLPRVTEIFEARKPKEPAVMAEISGKVEIRADRRRGKMTII
                     IRSEGGMEKEHHVPQDKQLLVHAGDFVEAGDPLVEGPLVPHDILRIKGEEALQNYLLS
                     EVQAVYRSQNVTINDKHLEIILMQMLRKVRIDNPGDTRFLPMEVVDKFRFRAENERLA
                     ASVKIKDPGDSTFTAGQIVPRTEFAEVVARIEQDGGTPPTRDRKPKPATAKTLLLGIT
                     KASLQSESFISAASFQETTKVLTEAAISGAVDRLVGLKENVILGHLIPAGTAFKPYLE
                     MRVKQVGEPLPEKQVTPEAAHVAMQEAGAAMAGMAAVTPMGPPVQALNISPIEEQRDH
                     QLGSVTG"
BASE COUNT         1230 a         2196 c         1963 g         1374 t
ORIGIN      
        1 tttccgggcc gcatcccccg cggaatcctt tccgcattcg gcattcatga cgatggcaat
       61 cttccaccag caggtagggc agatcaaagc cctgcgaagt cacacggacg aatcagggcg
      121 ccgacacctg cctggcattc ggaaccgagc ccccgcctgt ctgcgtcatc cggctccaac
      181 gacgcaacga cacaaccgtt ttggcgccgc cggaaagagt cgcgggcttg gcacgaagct
      241 gaacccgtcc cgcacccggt acgggggcaa catcattcgc gtgaccgcaa cggaagtccg
      301 ggccgttcgg atcggcaacc ggacggccgc cgatcgctat cgaaaaggcc gcctcaaacg
      361 cgccgacgac gcaatggtcc tcgaatgatg attattttgg agtaaatagg cttttgtgtg
      421 ctctatatca tccgttcttg agcagctctt ccatggtgac gacgcgttcc ttgtccatgg
      481 cctcgcgccc cagaatcgcg gccatcgccc catcgaaccc ggcttggacg tctgccagcg
      541 gcctgcgctt ctcccggatg catttgaaga aatcgtcgat cgccagttgc gtcgaatcgc
      601 cggccggctc cgccggcgtc accttgtagg gctcgccgaa gtttctcgga taaaccatgc
      661 cgttctcgac gtccaccgcc gcctcctcgc agtagacgat ctcctgccgg ccgtccatcg
      721 ggatcttcgg cgccgaaatc caggagtggc tgtacgaaac ctttttatcc ttcccatagt
      781 cgagcgagag ggcgtaattg tccatgatat cgcgaccctt cggctcgaaa cgcaggctct
      841 gctggccgaa gcccgccgca cgcaccggcg gcccgcccag aatccagttg aacaggtcga
      901 actcgtgcac cgcctgctcg acgatggtgt caccgcagta tcgcttctcg aaaagccatt
      961 gccggtcgtg cggcaggtcg cctacgttgt gccggtgggc acgtatgaaa atcggcttcc
     1021 ccacctcgcc gctgagaatc agcttgatgg acgactgcca gggatgcgca taacgcaact
     1081 gggttccccc ttggtagatg cccttagccg atcgagcggc ctccagcacg gccttgcagt
     1141 ccttgaccgt catacccagg ggcttctcgc aatagaggtg caacccggca cttaagaccg
     1201 ccaccgccat ttccgcgtgc aagtacacgg gcgtggcgat caccaccgca tccaagtctt
     1261 tgaattcgat gagtttgcgg tagtcctcaa agagccgcgg ctcgtcttgc tggaccttct
     1321 cggccgctgc ccggaggtgc ttggggtcaa tgtcgcacaa ggccgtgacg cgaacgccgg
     1381 gcgacttatt gacggcattg agcaagcccg aaccgcggcc gccgatcccg atcatgccac
     1441 aacgcaggtc gtccgccggc atcgtggtcg gctgggtatc ctcggcccga agtcgcacgg
     1501 cccccaacaa gacggcggac gcgaccccca aacctttctc cagtaccgct cgacgtgaaa
     1561 agtcactttt ttgatgcatc gatggtccgc tcctctcaac acaatggttg cttgcagcac
     1621 ttcggagcat tctaatcgag cagtcgaggc gcgaacagcc acagtgccga tctcagactg
     1681 tcgcttcgcc gccaggaatc gaccccgttg gaaatgccca aaccagcgta aagcatcgct
     1741 gtaacaggcg cgttttttgg ggaacccagg ggcgagcttc cgatccttgc cacgcccacg
     1801 tggtgtacct tatcatgaca tgtccccagg gtctcccggc gcgtgccacc cggggtatga
     1861 cgtgaacctg ccttcccgtg gaggtatctc cggatgatcc ctcaccgtcg gtctgcaagc
     1921 agttggggca ttctggccgt caccctgctt gcctttatta cacagggctg cgggcagatc
     1981 ctcaacgaca acctgttcag cagctttggc ggcaacgccg tggcggggct gccacgtccc
     2041 aatggcgcga ttatcatcct ggtcatgaac cagaccagct cggcggccgt ggtccgcgtg
     2101 caagtcaaca agactcgcta tgtccccggt gacgacacca cgggggacgg caccaccggc
     2161 ggtggaacca ccggcgacga cacgacaatc cccgccactt cactggagtt gagcatcccc
     2221 ctggagccct acgggcccgc gaccgagctc gaccatgcga tgatcgtgca ggaatgcgac
     2281 gttaccagta ttcaattcct tggaggcagc attcagagcg acaccggcgg agatccggtc
     2341 gagatacccg ccgacctgcc gccgatcacc tacggctcac agctcttttg cggagacgtg
     2401 gtcgcaatca ccatcgtcgg cgtgggcttg cagccggtga tttcgatata ctgacatgag
     2461 ccagaggttc catgtgatga aacggttaca gacactcagg ttcgcgacgc tcgtggcggc
     2521 cactctggcc gtccttgtga gttgcaccgg ctgcctgaac cccgagttgg tcaatcggat
     2581 caacgggcgg ctttatccga tcgtaccggg caatcagccg ttcctgctgg tccgagtgat
     2641 caataacacc aacgccatgt tggacgtgcc aatcgtctac aacgacggga cggccgatcg
     2701 cgaattcgtg atcaccggtc tgacgcccca ggtccacgag acgggtgtcg ttctggattg
     2761 gccggtgatg cggctggcac tctgcgatct ggacaatccc tacaacccaa acatcatcgc
     2821 cactctgccg gacggcagca tcagcgccgt gccgttcggc tacaatgctt tggaggccgg
     2881 tgtccactac atgtcgggcg acgggatcat cttctcgctg aacggagatg ttcgagcccc
     2941 ggcgttcgtg agggtaaaca tcggtcgtat cgatgcgcag acgcagccgg gcggctttac
     3001 cagggctgat ccttttgagg ccgccaatct gattctgctt ctgggcggct tttgatacgg
     3061 agcaaaccat gcgccgacga aagtttctgt tgttcggtct tctgacggca ctggtcggcc
     3121 tcccgatcat cattggggcc agttgcccgc cacccgatga cccggccttt gtgtcgatca
     3181 tcaccggccc gggctccacg atcggccagc aactgcccaa ctgcgaacga gggcgcgtct
     3241 gtatcagcat tcttaatcag acgtgcacgg acacggacgt catgctgtac gtccacgacg
     3301 gctacgacct gacgggtacg tatgtgactc gaagggccgt ggagtgctgc gagaatcaaa
     3361 acgctaccga gccctgcccg tgctaccgat gcggatcaaa tagcggtgaa atgcaactga
     3421 cgcctccgga actgtttcag ccgcagaatc gttacgaaat cagcaccggc caggtcatcc
     3481 gcaccatccc aggtcgccag gaccggctct cggccttggg aggccgcatc accatcagta
     3541 ttcgctgcga ggaggtcaag acaatcggtc tggaagtcgg tttggagggc gagctgcccg
     3601 gtctgatcca ggagcgcgcg ggccccgatt accgctgcac gagggtggat ctggattgtg
     3661 cacaggctta cacggaggat gtcgcctgtg gggcaaccat tcaatacacg atcgtcgatc
     3721 gcaacaactg cgccaaccag aatctcacgg tctttcggat cgaaacgaac gtttcagccg
     3781 attgtacgca ggtggaaaca accgataaca gcagcggcgg cggcggcatg agcggcggct
     3841 gaggatcggt gccatcgtcg aacctgaatg aggtactgcg gccggcattc ttgtcggccg
     3901 ccttcttctt tggcccctgg tcgacccgtg cggccagaga aaaaccccgg gcgatcttgc
     3961 ccggggttcc gttccgccta aaggtctcgg cgacctactt gcccttcttc acgccatact
     4021 tactgcggct gcgacgtcgt gggcggccgt ccttgtcgtt ctgcactccg gcacaatcca
     4081 acgtcccgcg aatgatgtgg taccgcacgc cgggcaagtc ccgcactctg ccgcctctga
     4141 tcaagacgat cgagtgctcc tgcaagttat ggtcgacccc tggaatatag gccgttactt
     4201 ccttgccgtt ggtcagccga acacgtgcca ccttgcgaag ggctgagttc ggtttcttcg
     4261 gcgtctgggt cttcacgatc aggcagacac cacgtttctg cggacattgg tccaggtcgc
     4321 ggacctttga cttacgcgcc tgtttggttc gaccgcgccg gataagctgg ttaatcgtcg
     4381 gcattccaat cctctgtgat catctcatgg acgccgggac ctccccccgg caacccaaat
     4441 acccatctta gcggatccgg gccggctttt ccagccctac cggtcgcctg cgactctgcg
     4501 gtccgcgacc gtccccgcct aacctgtcac cgaccccaac tggtgatctc tctgctcctc
     4561 gatggggctg atattcaggg cctgcacggg aggacccatc ggagtcactg cggccatccc
     4621 ggccatggca gccccggctt cttgcatggc gacgtgagcc gcctccggcg tcacttgctt
     4681 ttcaggcagc ggttcgccca cctgtttcac gcgcatctcc aggtatggct tgaacgccgt
     4741 tccggccggg atcaggtgtc ccagaatcac gttctctttc aggcccacga gcctgtcaac
     4801 cgctccgctg atcgccgcct cggtcagcac cttcgtcgtc tcctggaagg aggcagccga
     4861 gatgaacgac tcgctctgca gcgaggcctt ggtgatacca agcagtagag tcttggcggt
     4921 cgccggcttg ggtttccggt cgcgagtcgg tggcgttccg ccgtcctgct cgatcctggc
     4981 cacgacctcg gcgaactcgg tcctggggac gatctggccc gcggtaaacg tgctgtcacc
     5041 cggatccttg atcttgacgc tcgcggccag ccgctcgttc tcagcccgga accggaactt
     5101 gtccactacc tccatcggca ggaagcgagt atcccccgga ttgtcgatcc ggactttccg
     5161 gagcatctgc atcagaataa tctccaggtg cttgtcgttg atcgtcacgt tctggctgcg
     5221 gtagacagcc tgaacctccg agagcaggta gttctgcagg gcctcctcgc ccttgatccg
     5281 caggatgtcg tgcggcacca gcggcccctc gacgagcggg tcgcccgcct cgacaaagtc
     5341 gccggcgtga accagcaact gcttgtcctg cggaacgtgg tgttcctttt ccatgccgcc
     5401 ctcggaacga atgatgatag tcatcttgcc acggcgacga tcggcgcgaa tctcgacctt
     5461 cccggagatt tcggccatga ccgccggctc cttgggcttg cgggcctcga agatctcggt
     5521 gacccgcggc aggccgccgg tgatgtcctg cgttccggcg atggcccgcg gctggcgggc
     5581 caggagtgta cccggaacga tcttgtcgcc ctctgcgact tcgatacgtg ccttggcggg
     5641 tagatagtgg taatcgagca cgcggccttc gacgtcctcg atgacgatct ggggatgttt
     5701 ctcgcccttg tgttcgacga cgatgtactt ggcgccggcc cgctcctgtt cgaggcgcac
     5761 cgtctcgcct tcggtaatgt catggaagcg gacgactccc gagacctcgg ccaagatcgg
     5821 cgtcaagtgt ggatcccatg tggccagttc ggtccgcgga ccgacttcct ggccttccct
     5881 taccaccagc agggccccgt aaggcacctt gtaacgttca agctcgcggt tcttggcgtc
     5941 gacaatggcc acttcaccgt tacgcttgag ggccacttgg cgggtcccct cgccctccgt
     6001 cgccggaacc tcgaccgcgt tcagatcgtg gaagaccacg cgaccggcct ggatgttgat
     6061 gatcttgttc tctgccaccg cgcgggtcgc gacaccgccc gtatggaacg tgcgcatcgt
     6121 aagctgcgtt cccggctcgc cgatcgactg ggcggcgaga ataccgaccg ccatgccctc
     6181 ctcgaccaga ttgccggtcg acatgtcaac cccgtaacac cgggcgcaca caccgaacgg
     6241 actctcgcag gtcaagggac tgcgaacacg aatcgtatcc aggccgagtt cctcaatctt
     6301 ggcggcgatc tcacgcgtaa taacgtcgtt ctccgccacg ataatctgat ccgtgacggg
     6361 gttgcggatg gtgtcgcggg cgacacgacc gatgatgctg tcccgcagcg gcacggcgat
     6421 ctcctcgccc ttgtacaccg cgcccttggt aatgccgttg atggttccgc agtccagtcg
     6481 ggtgacaatg acattctggg ccacgtcggc cagcttgcgg gtcaggtatc ccgagtccgc
     6541 ggtcttgaga gcggtatcgg ccagaccctt tcgggcgccg tgcgtcgagc tgaaatacgc
     6601 cagcaccgac aggccctcgc ggaaattgct cttgatcggc gtctcgatga tctcgcccga
     6661 cggtttggcc atcagcgccc gcatgccggc aagctgacgg atctggtcca ccgatccgcg
     6721 ggctcctgaa tcggtcatga ggtaaatggg gttgagatag aag
//
LOCUS       DAOTPW010000288         6756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9778_length_6756_cov_4.650649, whole genome shotgun sequence.
ACCESSION   DAOTPW010000288 DAOTPW010000000
VERSION     DAOTPW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9778_length_6756_cov_4.650649"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="P5316_19200"
     CDS             complement(<1..1102)
                     /locus_tag="P5316_19200"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87143.1"
                     /translation="MRTTPSRRLEWRFAAAIGLMFAATALSHPLGNDNVEHVNVLWIF
                     PDRVEVDCLLFIAENQSSRIKQDEIDTDKDGFASTDEQQAWLELEAGRQKDRLRVTLD
                     GRPLPLRTVEAPVDPNSGMRSFSNRLIIEQPGLGGMPTYRLLIRYVTRFDRPLTDAVH
                     MLTFEDSSYLSSPGLKRILLERLPDIEVLPPHPEFWTTDPFLFEQYDPANLPQERSAI
                     IQFRLAADAGIPATGPTQEKTVSGRPHAAGRPDVPDRYEKGLTAPVAGVQTTYQAQAN
                     RLMELLQLRWGLMVFVMITGLAFTWGAAHALMPGHAKTVVAAYLISQSGTYWHAVLLA
                     VVVTITHTALVVALGVIWAFYQATNPALGPKVQ"
     gene            complement(1162..3429)
                     /locus_tag="P5316_19205"
     CDS             complement(1162..3429)
                     /locus_tag="P5316_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87144.1"
                     /translation="MQDDKPLAMAPAQVALHNLGDLLKSNLAAFVAAAIVVVVLAILY
                     FTTESLQPPQAKLNSQIEVEVEKAKRMLANYNPVGERVTTLIDTAFASSTQPMTEPVP
                     EDQWPVSLKTLREHLDSVAGQIEPLNVRYRAWSGETPAPGTAAGYSQLKEQLEKNLAQ
                     VSEALKIVQSAVNMSVEAGDRTISGRDHPVATRLEAVLIYHQADLLRQKAAARRDHAV
                     TARAEFERARAQWQMLQSQIAAIQAVAHREAASVPAAARAPAVPQSEPPKAATRPPEK
                     TVPAGKPGVVSRLTGLLFGKKKPDAVPPDSTSPEPAPAQADRETKEPMVESPEPPAPT
                     AFVKPPTPQQRLRELAAQREQAIIQIAAAEADVARLRDHVSDLEHALAGAKQAARDAQ
                     LRMMRLEEQGVDPTDPEAIERFKSDYQQASTEYRKQVREAALLENGGIRNARPDTQDE
                     REYLNAPLVSADPAQPMNPTEGLVSVRKRLQAAEEQHEAAKNLLATIDVQVAAVQRRQ
                     SDDSARLTALEQRQAALKEQAAKAIKDAVVATAEADKLARDAIELLSGDGRQAAQNAR
                     TAADNQIREARNRQQPGVENPRLEIITKSGMLVGSAQTLEADIALLTAYVQAARVSDL
                     LAHAQVLSHAESMGVRPQQPQDDAALAYEAAAAAEEARKAREQAIAVAREAYNSYREA
                     SAALDNLWVLNANAAAVCYLLSSLQTGEEADKSRDLAIRIYEQTLQGRSDRKDIADYR
                     RALDGLNRLRQARAR"
     gene            complement(3645..5276)
                     /locus_tag="P5316_19210"
     CDS             complement(3645..5276)
                     /locus_tag="P5316_19210"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRR87145.1"
                     /translation="MNTWRVLRTLLTLTAVASPPAIALDINNPPVGTFVDEWYVVRIE
                     GKKSGHAHITMKRSQKTGDDVITSRTDMVIEISRAGQEAGVRLVQQSEETIDGKPLRF
                     MNTIRLGALPGGITTRGTVADGKVTITTSQFGQETEPRVYPMPEGAMMSWAVYREQMK
                     RGLKPGDKYELMAYEPSISADKVIPMSVEIMEPETIDLFGRKVQATKTSQVMHITNML
                     GQKSDVETLSWMTDDGNIVRSQMMVAKWKIEILAASKSVALSPNEPTELMTDALIPVS
                     RPIDGRSDRITYRISLKNKVDRVRLSDIPETDMQRVLRRNESELDLVVTRRTSEGDRP
                     GAKKLGDSPARRLAAKEKERYLTPSSVVNFKDPTVADLAKQAAGDEKDPLKLAERLCQ
                     FVSDYVEDKNLNVGFATASETARSKEGDCTEHGVLLAALGRAVGIPTRLVTGIVYARH
                     FAGKEGVFVGHLWTQFFIDGRWVDLDSALGQTVADPTHIALSLSDAGDSGIADMVSSV
                     WLNMGELKIEVLDGPGRKAISQPAGLVPASVPTLR"
     gene            5762..5835
                     /locus_tag="P5316_19215"
     tRNA            5762..5835
                     /locus_tag="P5316_19215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5796..5798,aa:Arg,seq:acg)
     gene            6197..6487
                     /locus_tag="P5316_19220"
     CDS             6197..6487
                     /locus_tag="P5316_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87146.1"
                     /translation="MDLKVLPIHHRLVDQVRAHVLPCILAYRVWWHMRQVPAPIVFDD
                     DEEGTGEADVGSGRGPAVGASRGQGPEEADRRRRAGAQLPDALGGLGDAGEE"
BASE COUNT         1134 a         1998 c         2212 g         1412 t
ORIGIN      
        1 gctggacttt gggaccgagg gccgggttgg tcgcttggta gaatgcccag atgaccccga
       61 gtgccacgac gagtgccgta tgggtgatcg tgacgacgac ggccaaaagt acagcgtgcc
      121 aataggtgcc gctctggctg atcagatagg ctgccacgac ggtctttgcg tggcctggca
      181 tcaatgcgtg ggcggcgccc cacgtgaacg ccagtcctgt gatcatgacg aacaccatca
      241 ggccccagcg gagctgtagc agttccatga gtcggttcgc ctgggcctga taggtcgtct
      301 gtacacccgc cacgggggcg gtgaggcctt tctcgtatcg atcgggcacg tcggggcgtc
      361 ccgctgcgtg gggacgaccg ctcacggtct tttcttgcgt ggggcccgta gccggaatac
      421 ccgcatcggc ggcgagtcga aactgtatga tcgctgagcg ctcctgtggc aggttagcgg
      481 ggtcatactg ctcaaagaga aacggatccg tggtccagaa ctcgggatgc ggcggcagca
      541 cttcgatgtc cggcagacgt tcgagcagga tgcgcttgag gccggggctg ctcaggtagc
      601 ttgaatcctc aaaggtcagc atgtgtaccg cgtccgttaa tggacgatca aagcgggtga
      661 cgtagcggat cagcaggcgg taggtgggca tgccgccaag gccgggctgc tcaatgatga
      721 ggcggttgga gaaagatcgc atgccgctgt ttgggtcaac aggggcttcg accgtcctga
      781 gcggcaatgg tcggccgtcg agggtaacgc gcaacctgtc tttctggcgg ccggcttcca
      841 gctccagcca ggcttgctgc tcatccgtgg aggcgaatcc gtccttgtcg gtgtcgattt
      901 catcctgctt gatgcgggag gattggtttt cggcgatgaa cagcagacaa tccacctcga
      961 ctcgatcggg gaagatccag agcacgttga catgctcgac attgtcattg ccgaggggat
     1021 gagagagagc cgttgcggcg aacatcagcc caatcgccgc cgcgaaccgc cactcaagcc
     1081 ggcgtgaagg tgttgttctc atcgggaccg cagccgcgaa gcgatacaag tagcccgcag
     1141 ggacttgctc atgccatcgc atcacctagc ccgggcctgc cggagccggt tgagcccgtc
     1201 gagcgcccgg cgataatccg cgatatcctt ccgatccgat cgaccctgca aggtctgctc
     1261 gtagatccga atcgcgagat ctcggctctt gtcggcttcc tccccggtct ggagcgatga
     1321 caacaggtag caaacggcgg ccgcattcgc gttcaggacc caaagattgt cgagggccgc
     1381 agatgcttcg cggtagctgt tgtaagcctc acgggcgacg gcaatggcct gctcgcgggc
     1441 cttcctggcc tcttccgccg cggcagcggc ttcataagcg agggccgcat cgtcctgcgg
     1501 ttgctgcggg cgaacgccca tgctctcagc gtggctcaga acctgggcat gagccagcaa
     1561 gtcgctgacc cttgcggcct gaacgtaggc ggtcaacagg gcgatatcgg cctcgagcgt
     1621 ttgggcagag ccgacaagca taccgctctt ggtgatgatc tcaagacgtg ggttttcgac
     1681 gcccggctgc tgcctgttgc gagcctcacg gatctggttg tcggcggccg tgcgcgcgtt
     1741 ctgcgctgct tgtctgccgt ctccgctcag cagttcgatc gcatccctgg ccagcttgtc
     1801 ggcctcggcc gtggctacaa cggcatcttt gatcgccttg gcggcctgtt ccttcaatgc
     1861 cgcctgtctc tgttcaaggg cggtcaaccg ggcggagtcg tcgctttgcc gacgttgaac
     1921 cgcggcgacc tggacatcga tagttgccag caggttcttg gctgcttcgt gctgttcctc
     1981 cgccgcctgc aaccgctttc ggacggagac caagccttcg gtggggttca tgggttgcgc
     2041 cggatcggcc gagaccaacg gggcattcag gtactcccgc tcatcttgag tgtccggcct
     2101 tgcattacga atgccgccat tttcgagcag cgccgcttcc cgcacctgct tgcgatactc
     2161 ggtcgaggcc tgctgataat cgctcttgaa ccgctctatt gcttcgggat cggtggggtc
     2221 aactccctgt tcttcgagcc gcatcatgcg gagctgggcg tcgcgggcag cctgcttggc
     2281 gccggcaaga gcatgttcca ggtcagatac gtggtctcgc agtctggcca cgtctgcctc
     2341 ggcggcagcg atttgaatga ttgcctgttc tctttgggcg gccagttcgc gcagccgttg
     2401 ttgcggcgtc ggaggcttca cgaacgccgt gggagccggg ggctccggac tctcgaccat
     2461 cggctccttg gtttctctgt cagcctgggc aggcgccggc tcgggtgacg tcgaatccgg
     2521 cgggacggca tcgggctttt tcttgccgaa gagaaggccg gtcagccggc tcacgacgcc
     2581 cggcttgcct gccggcacgg ttttctcggg tggccgggtt gccgccttcg gaggttccga
     2641 ttgaggcacg gccggcgcgc gggctgcggc gggcactgag gcagcctctc tatgggctac
     2701 cgcctggatc gcagcgatct gggattgaag catctgccac tgggctcggg cccgctcgaa
     2761 ctctgccctc gcggtgacgg cgtgatcgcg gcgagccgct gctttttgac gcagcaggtc
     2821 cgcctgatgg tagatcagga cggcctctaa ccgggtggcg acggggtggt ccctgccgct
     2881 gatggtccgg tcacctgcct cgacggacat gttgacagcg gattggacga tcttcagggc
     2941 ctcgctgacc tgagcaaggt tcttctccaa ctgttctttc aattgggaat agcccgccgc
     3001 cgtaccgggt gcgggcgtct caccgctcca agcccggtac cgtacgttca gaggctcgat
     3061 ctggccggca accgaatcga ggtgttcccg cagcgtcttc aggctgacag gccattgatc
     3121 ctcgggaaca ggttcggtca tcggctgcgt tgaggaggca aatgcggtgt caatgagggt
     3181 ggtcacccgc tccccaacgg ggttgtagtt ggcgagcatt cgcttggcct tttcgacctc
     3241 gacctcgatc tgactgttga gtttcgcctg gggaggttgc aggctttcgg tcgtaaagta
     3301 caggatcgcc agtacaacga ccacaatggc ggcagccacg aatgctgcga ggttcgattt
     3361 gaggagatcg ccgaggttgt gaagggcgac ctgagcgggg gccatggcca gaggtttgtc
     3421 gtcttgcact tgggggctcc ggtgtagggt acgggccggt catccatccg gggcggtgcc
     3481 ccccgcaacc ctgcttcggc acttcggggg cggggaggga accacatcag gcagggggct
     3541 tgtttgtcgc ccccctaccc ttcatctgta ccagtttcgg cccggagcgt caacgtacaa
     3601 agcaaccgct gcaaccgttg gcggctggga gccgggtctt ccggctatcg cagggtgggc
     3661 acggacgcgg gtaccaggcc ggccggttgg ctgatggcct ttcggccggg cccatccaga
     3721 acttcgatct tcagctcgcc catgttaagc cataccgaac tgaccatatc ggcgatgccg
     3781 ctgtcgccgg cgtcgctgag gctcagggcg atgtgggtcg ggtcggctac ggtctggccg
     3841 agggccgagt ccaggtcgac ccaccggccg tcgatgaaga actgggtcca gaggtgcccc
     3901 acgaagacgc cttccttgcc ggcaaaatgc ctggcgtaga cgatcccggt gaccagccga
     3961 gtggggatgc ccacggctct acccagggcc gccaagagca ccccgtgctc ggtgcagtcg
     4021 ccttctttgc tgcgggccgt ctcactggcc gtggcgaaac cgacgttcag gttcttgtcc
     4081 tcgacatagt cactaacgaa ttgacatagt ctctcggcca gtttgagggg gtctttctcg
     4141 tcaccggcgg cctgtttggc cagatcggcg acggtcgggt ccttgaaatt gaccaccgat
     4201 gaaggcgtca gatacctttc tttttccttt gcagccagtc ttcgggccgg cgagtcgccc
     4261 agcttcttgg cgcctggcct gtcaccttcg gaggttctgc gagtcacgac cagatcaagt
     4321 tccgactcgt tgcggcgaag tactcgctgc atgtccgtct cgggtatgtc gctgaggcga
     4381 acgcgatcga ccttgttttt aagagagatt cggtaggtga ttcggtcgga cctgccatcg
     4441 atcggcctgc ttacgggaat caatgcatcg gtcatcagtt cggtcggttc gttgggcgac
     4501 agagcgacgg atttgctggc cgccaggatc tcgattttcc atttcgcgac catcatctgg
     4561 ctgcggacga tgtttccatc gtcggtcatc cagctgagcg tctcgacgtc cgacttctgg
     4621 cccagcatgt tggtgatatg catcacctgg ctggtcttgg ttgcctgaac cttgcggccg
     4681 aagagatcga tcgtttcggg ttccatgatc tcgaccgaca tgggaatcac cttgtcggcg
     4741 gatatgctcg gttcgtatgc catcagttcg tacttgtcgc cgggcttgag cccgcgtttc
     4801 atctgctccc gataaacggc ccagctcatc atcgcgcctt cgggcatggg gtagactctc
     4861 ggctcggttt cctggccgaa ctgggaagta gtgatggtta cctttccatc ggcgacggtg
     4921 ccgcgggtgg tgatgccgcc gggcagagcg ccgagtcgga tggtattcat gaatcggagg
     4981 ggcttgccat caatggtctc ctcgctttgc tgaacgaggc ggacgccggc ttcctgaccg
     5041 gcccgactga tttcgatcac catatctgtg cgactggtga tcacgtcgtc gccggttttc
     5101 tgtgagcgct tcatggttat gtgcgcgtgg ccgctcttct tgccttcgat tctcaccacg
     5161 taccattcgt cgacgaacgt accaaccgga ggattgttga tgtccagcgc gatggccgga
     5221 ggagaggcaa cggcggtcag agtcaacaac gtgcggagca ctcgccaggt attcatgtga
     5281 gcaccatgat aaagataaca accccgtggg ccgggggcgg acggcaattg tatccccggc
     5341 cggacgatcc gagtgtatcg gagggggagt cggccggcaa tccgacaagc tgttgccgcg
     5401 agaggatggc actggtcgtt ttttcgtcgg gtctggactt gggcagttcg cggtcgagca
     5461 ccgaaccctt tggggctttg caggccgcta tcagcccata ttgcaccggc cgttcgttca
     5521 acggcctctt gtgacgtcca agccggcacg tgcggacaag tcacccggaa aaccgacatt
     5581 ggccggcggc cgaggcttgc cgcggaccgg agtgaggctg accacgcccc gcaagcgtct
     5641 tggtgaccgg cccgtcgccc gctcgcaaat ccgtgcggca acgagtcttg atgccgcgat
     5701 actggacacc cttggcgcgg gacgatatca ttatgtgctc aaccggcgcc aatcgccgta
     5761 agcaccgcta gctcaactgg atagagcatc ggtctacgga accgaaggtt gcaggttcga
     5821 atcctgcgcg gtgtagttca gatcccttgg tgcctggcga tgccagtgaa tacctggaaa
     5881 tgttggatca ctccatctta tccggatttg cgatttccct cgtggcaagg ggttccgtag
     5941 tacctgcggc ctgaagctgt tggttgtgat gaaccgagta tcggcacccg tgcaccgctg
     6001 ccgggcaagt aagagggcag gtctttggcg gctccgtcgc gtccaggaga agtccagtcg
     6061 cgccttctgc gttggccatc cggtgtcgct tcggctcgga gatgtccggc tcggaaaggc
     6121 gttcaaggcc ctcgctccgt cgcctacgga caagtagctg caccgggcca tcgtcaaaac
     6181 gggtcgcctc gagacggtgg acctgaaggt gctgccgatc catcatcgtc tggtggatca
     6241 ggtgcgggcg cacgtgctgc cgtgcatact ggcgtaccgc gtgtggtggc acatgcggca
     6301 ggtgccggca ccaatcgtgt tcgacgacga cgaagaggga acgggcgagg cggacgtcgg
     6361 tagtggccga gggccagcgg tcggcgcgag ccgaggccaa ggccctgagg aagcagaccg
     6421 ccgacggcga gcgggtgcac agcttcccga tgctcttggc ggacttggcg acgccggcga
     6481 agagtagcgt tcggttccaa ggcttggaga cggccagaat gaccgaccgt ctacgcccaa
     6541 ccgacgccgc tccagaaacg cgcgctggga ctgttggaca gggcaaccgg aatacgggca
     6601 gccaggggaa gctcagcatc gcgagaactc ccgcaaccac ggccgattac gcgttttgca
     6661 ggcgtgggac ttcggcttga accggccttc gagaaggccg gtttgcggtg caaaggcgaa
     6721 gaattctggg ggaaaacacc cgtaaagcgc gcggat
//
LOCUS       DAOTPW010000289         6631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_9986_length_6631_cov_5.020529, whole genome shotgun sequence.
ACCESSION   DAOTPW010000289 DAOTPW010000000
VERSION     DAOTPW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9986_length_6631_cov_5.020529"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..763
                     /locus_tag="P5316_19225"
     CDS             128..763
                     /locus_tag="P5316_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87147.1"
                     /translation="MNQEDFSGLEGQPQTQTPPPLTPPIQPGPLARPAGYSPYSSPIP
                     VWPTVIGVVGIVLGSLGLLGAIWGAVAPLVMKTLFPNMAADPDMPHFSERWIQSTIIS
                     GVVSSAVAIILIVAGAGMLKRTAWSPRVARIWAVIKMVLVVANLVVAFQIQQSTMANM
                     AQVGGRGMVVNVNFLTIPSILFGLLWGWALPVFLLIWFSRASIKEQVSKWT"
     gene            838..2328
                     /locus_tag="P5316_19230"
     CDS             838..2328
                     /locus_tag="P5316_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87148.1"
                     /translation="MRCKTCEYRLWNLPARRCPECGTPFKPSDYEFTINSVQFCCPHC
                     GQSYYGTGERGHLVPVEFDCVGCGRHIHMDEMVLLPTEGVEENQTRIENAPWLERAAR
                     GRVRAWFSTVGMALIRPARLMEMLPEEGATGQAMLFGIITTLLVVPLALWPFFLLPLV
                     FFVSGFGGLGLGEVAGLMLVAIVAAAVVLVSMFVWALTIHGLLRITGETAGGLGRTYQ
                     AVFYGSGANVGSALPCMGIYVGWIWYVISTAIMVKSRQRVSGLRAAFATAAFPVSLLV
                     AIIGFYVWFFLSVIPGIPPAGPAAGFPFAATMPVGQMNRTVLDAILIYAGDHDGAGPD
                     HAAELVARGYLRASDLVLPGSQTKADQIVIGETTLVGLESMRREPARKEARAASAKLP
                     PNTVAHRLGDWVFTCHGVTLNPPPDGRIWLVVQCLDPDANAEAFSSDVIAWCADGTMV
                     VLPDSELAAGLRDQNSLRRRLGLSRLPDPRQVTHGRPAVNDEARKR"
     gene            complement(2351..4138)
                     /locus_tag="P5316_19235"
     CDS             complement(2351..4138)
                     /locus_tag="P5316_19235"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRR87149.1"
                     /translation="MLCQTVSGNRVYQALAIWVWAACSTFLVAQESPYLYGIHDHSPS
                     PQEYLNHLTGGGVTGWITATVAVGHNPAETWGIDFRSFSNQGHTVICRINNGYCESGG
                     TIPLPDQYANFATRCANFVSNSQGCSIWVIGNETNLAGEWPPVGSHKPYVSPQSYADC
                     FRQVYNAIKAVRPVHKVCTQAIAPFGGPYGPGSVCGYTHDGNPINWVQYMNQMLTTIQ
                     GSGPGPDGIAVHINSRGYDYADIHSTQKVNAAGQLLYFSFYVYKDWINYGIPSSMYDL
                     PLYATECNGLYSWDGTYPDSEPGREPYQAGWMQEIYAEINRWNTIDAPAAGKPVFRCV
                     NMYRWSGDPWRIDGIPQKTQILADLDAAVAQKYRSDVVFTSNPPTGTNLAPYSLTVQT
                     DSVYGPDWSGSNAIDGVVSVSSKWVSAATAPPHWLALDLGGNRTVDGYIIRLAGAAGE
                     PTSYNAEALAIQTATSLSGPWATEGTIDNSIRASIINRSYVTPKTLRYVRLYITDAGV
                     DNHARIPEFEVWGVFPGYRGDMDGDEDVDQADFGLFQACYTTAGNPISPACTAAELDH
                     DDVIGQSDFALFVQCLCGEGIAPPISCLR"
     gene            4384..4944
                     /locus_tag="P5316_19240"
     CDS             4384..4944
                     /locus_tag="P5316_19240"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR87150.1"
                     /translation="MFRLGLKRLVAPLAEFIEQRLPAVETAGTATAREVLRPPGALPE
                     PEFLARCYRCGNCVEVCPAKAIHPVNRQNIERAGTPYIDPDLAACTLCDQLACMKACP
                     SGALQVIGDKRQIKMGTAAVDRRLCLRRSSSLCTICLDTCPIGREAIDISDGGELQVN
                     PMACVGCGTCQYNCPVRPRAIRVVPR"
     gene            5486..>6631
                     /locus_tag="P5316_19245"
     CDS             5486..>6631
                     /locus_tag="P5316_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87151.1"
                     /translation="MRKFMLASTVVWFIVGQTMGASTVTMELELGGSNNGATCATPIY
                     FTPGSTEDGQFALDENGRLTWAVRIHASGTHLDASQQEVAIRGVANFVFDLELYHDYV
                     DPANLVTTATFMSTANQEMTNCGVEQNWSNVPAGMAYLSNAAFAVVFDTNTSTSVGPG
                     RIVDYYRLTDGHPGGGPRMDVRCYPKAAAGEGKLQGVGAGYSLWNKSSLSLLTVGGIG
                     RGDFASIPGPGRPYGYLANTETKASGDPSYGWLGYGPVVEGQIEGLQPGITYILRLVP
                     GGGINVLRGGTQTQTSAEVFAIAADTTVGDDITFTIAPVQPPVQILGWSSVRDCGGVF
                     RAIPLNPTRSVGQVLTTETRKDGLQMIQIDFDGDITGRHVPGQISLSN"
BASE COUNT         1246 a         1969 c         2039 g         1377 t
ORIGIN      
        1 ccgccggcga tggtgctcgc cacggctgta caaatcgcag tgcaaatcag gtatcatctg
       61 cctttgcagg tcttttcgtt cggccggccc ggacggcgag cgtatgcgtg ttcgttaagg
      121 aggaaccatg aatcaggaag acttctctgg ccttgagggg cagccccaga cgcagacgcc
      181 tcccccgttg accccgccga tccagccggg cccgctcgcc cggcccgcag gctattctcc
      241 atactcatct ccgataccgg tctggccgac cgttattggc gtcgtcggga ttgtgcttgg
      301 cagtctgggc ctgttgggcg caatctgggg agccgtggct cccctggtca tgaaaacgct
      361 ctttccgaac atggccgccg acccggatat gccgcacttc tccgagcggt ggatccaatc
      421 gaccatcata tcgggtgtcg tctccagcgc ggtggccatc attctgattg tggcgggtgc
      481 gggaatgctt aaacgaacgg cctggagccc tcgggttgct cgaatctggg ccgtcatcaa
      541 aatggtcctt gtggtcgcta accttgtcgt ggcctttcag atccaacaat cgaccatggc
      601 aaatatggcc caggtcggcg ggcgcggaat ggtcgtcaac gtcaacttcc tcacgatccc
      661 cagcatcttg tttgggttgc tctgggggtg ggcgctgccc gtgtttctgc tgatttggtt
      721 cagtcgcgcg agcattaagg aacaggtgtc aaagtggacc tgagcggtgc gacagcagca
      781 gcggtgatcc aaaggcaaga ctgtgcaacc gtttgagagg cgacgcgtcc aacgacaatg
      841 agatgcaaga cgtgtgagta tcgactgtgg aatctgccgg cgcgccgctg cccggaatgc
      901 ggcaccccct tcaagccgag cgactacgag ttcaccatca acagcgtgca gttctgctgc
      961 ccgcactgtg gccagtcata ctacggcacc ggcgagcgag gccatcttgt ccccgtggaa
     1021 ttcgattgtg tcggctgtgg ccggcatatc cacatggacg aaatggtgct gcttccgacc
     1081 gagggcgtcg aggagaatca gaccaggatc gaaaacgctc cctggcttga gcgcgcggcg
     1141 cggggtcggg tcagggcatg gttctcgacc gtgggcatgg ctttgattcg tcccgcccgt
     1201 ctcatggaga tgttgcctga ggagggtgcc actgggcagg cgatgctgtt cggcatcatt
     1261 actactctcc tcgttgtgcc gctggccctg tggccgttct ttctcttgcc ccttgtgttt
     1321 ttcgtaagcg gttttggcgg cctcgggttg ggtgaggtag ccggcttgat gctcgtggcg
     1381 atcgtcgccg ccgcagtcgt gttggtgtca atgtttgtat gggcgttgac catccatggg
     1441 ctgctgcgga taacgggcga aacggcaggg ggattgggcc gtacatatca ggcggtattc
     1501 tacggcagtg gtgcgaacgt cggcagcgct ctgccctgca tgggtatcta cgtcgggtgg
     1561 atctggtacg tgatcagcac ggcgatcatg gtcaagtcga gacagcgcgt ctcgggcctg
     1621 cgggcggcgt tcgcaacggc tgcattcccg gtgtcgcttc ttgtggcgat catcggattc
     1681 tacgtctggt tcttcttgtc cgtaatccct ggcattcccc ccgcgggacc ggcggctggg
     1741 ttcccgttcg ccgccacgat gcccgttggg caaatgaacc gcacggttct tgacgccatt
     1801 ttgatctatg ccggcgatca tgacggggca ggacctgacc acgccgctga gctggtggcc
     1861 cgcgggtatc tgcgcgcgtc cgacctggtc ctccccggat cacagacgaa ggccgaccag
     1921 atcgtgattg gcgagacgac gttggtcggt cttgaatcga tgcgccgcga gccggcacga
     1981 aaggaagcca gggcggcctc ggcgaaatta ccgccgaaca ccgttgccca tcgcctgggc
     2041 gattgggtat tcacgtgcca cggcgttaca ctgaacccgc cgccggacgg tcgtatctgg
     2101 ctcgtcgttc aatgcctcga cccggacgca aatgcggaag ctttttcgag cgatgtgatt
     2161 gcatggtgtg cggacggaac gatggtggtt ctacccgatt cagaattggc tgccgggctc
     2221 cgcgatcaga actccctcag acgacgcctc ggtctgtccc gtcttcccga cccccgccag
     2281 gtcactcacg gccggcccgc ggtaaacgat gaggcgagga aacgataggg cagccgccgc
     2341 gcggccgatc tcacctgaga catgaaatgg gtggcgcgat cccctcgccg cagaggcatt
     2401 gcacgaacag agcgaagtcg gactggccga tgacatcatc atgatccagc tcagcggcgg
     2461 tgcaagccgg tgatatcggg tttccagcag tcgtgtagca ggcctgaaac aacccgaagt
     2521 ccgcctggtc cacgtcttca tctccgtcca tatcgccgcg ataacccgga aagacccccc
     2581 aaacctcaaa ctcggggatt cgggcgtggt tgtcgacccc cgcatcggta atgtataagc
     2641 gaacgtagcg aagggttttg ggagtgacgt agctgcggtt gatgatgctg gcccggatac
     2701 tgttatcgat tgtgccttcg gtcgcccacg gaccagagag gctcgtggcg gtctggattg
     2761 ccagagcttc agcgttgtag ctggtcggct ccccggcggc ccccgccagg cggatgatgt
     2821 acccgtccac cgtccggttg ccccccaggt cgagagccag ccaatgcggt ggggcggtgg
     2881 ccgcactgac ccacttgctg ctcaccgaga caaccccatc aattgcattg ctccccgacc
     2941 agtcaggccc gtacaccgag tccgtctgca ctgtcaggga gtacggggcg agattcgttc
     3001 ccgtcggcgg gttgctggtg aacacgacgt cggagcggta cttctgagct acggcggcgt
     3061 caagatcggc aaggatttgc gtcttctgag gaatgccgtc gattctccat gggtccccgg
     3121 accatcgata catgttgaca caccggaaaa ccggcttgcc ggcagccggc gcatctatcg
     3181 tgttccaccg gttgatctcg gcatagatct cctgcatcca gccggcttga tacggctcac
     3241 ggcccggctc gctgtccggg taggtcccgt cccaggaata cagaccgttg cactccgtcg
     3301 cgtacagcgg cagatcgtac atgctgctgg gaattccgta gttgatccag tccttgtaga
     3361 cgtagaagct gaaatacagc aactggccgg cagcattgac tttctgcgta ctgtgaatgt
     3421 ctgcgtagtc gtagccgcgg gagttgatat gcacggcaat cccgtcaggt cccgggccac
     3481 tgccctggat tgtggtcagc atctggttca tgtattgaac ccagttgatc gggttgccgt
     3541 cgtgggtgta tccacacacg ctgccgggac cgtaaggccc gccaaagggg gcgatggctt
     3601 gggtgcagac tttgtgcacc ggccgaaccg ccttgatcgc gttgtaaacc tgccggaaac
     3661 agtcggcgta gctctgaggt gaaacatagg gcttgtggct gcccaccggt ggccattcgc
     3721 cggcgagatt ggtctcgtta ccaatcaccc agatgctgca gccttggctg ttggagacga
     3781 agttggcaca gcgcgtggcg aagttggcgt attggtccgg cagggggata gtgccgcccg
     3841 attcgcaata cccgttgttg attcggcaga tgacggtatg gccttggttc gagaacgagc
     3901 ggaagtcgat cccccacgtc tcggccgggt tgtgaccaac ggccacggtc gccgtgatcc
     3961 agccggtgac accgccgccg gtcaagtggt tgaggtattc ctgtggcgac ggcgagtgat
     4021 catggatccc atagagatac gggctttcct gagcgacaag gaacgtacta caagccgccc
     4081 aaacccagat cgccagcgcc tggtacaccc ggttgccact caccgtttga caaagcatct
     4141 gattccctcc cgaccggcta acggagtctt gcctgctacc caatcataca ccgtgacatc
     4201 tggccatgct gcgcctctca gaaaaccccg gacgaaagcc ttcggactgg ccccccggtt
     4261 cttgtttgcc caagccattt gcgtctcgta acatcagatc agccttgaat cggcgattgg
     4321 gatgatcaac ggagactgca tttggccggc aatccatctg acacaccgca cgaccgcagg
     4381 gccatgtttc ggcttggcct caagaggctt gtcgcccccc tcgcggagtt cattgaacag
     4441 cggctgccgg ccgttgaaac cgccgggacc gcaaccgccc gtgaggttct ccggcccccc
     4501 ggagccttgc ccgagcctga gttcctggcc agatgctatc gttgcggcaa ctgcgtcgag
     4561 gtctgccccg ccaaggcgat tcaccccgtg aaccgccaga acatcgagag ggccggcacg
     4621 ccctatatcg accctgactt ggcggcgtgt acgctgtgtg accagttggc gtgcatgaag
     4681 gcctgcccca gcggggcctt gcaggtgatc ggcgataaac gccagatcaa aatgggaacg
     4741 gctgcagtcg accgccggct gtgtctgaga cgatcaagca gtctctgcac gatctgcctg
     4801 gatacgtgtc cgattggcag ggaagccatc gacatttccg acggcggcga gctgcaggtg
     4861 aaccccatgg cctgcgttgg ctgcggaacc tgccagtaca actgccccgt acgtcctcgg
     4921 gccatcaggg ttgtgcctcg ctgacaactg gccaaccaca gacccaacgc cggcgataaa
     4981 tgcatgcctg accggcctgg aacgccgacc cccaaaccac atcttccgcg ccggcgatga
     5041 acgtatctcg gactccccat gccggatgcc ggacgttctg ttttgtgctc ctgattagtg
     5101 tgtggagacg cttcaacgac aaaaaatgtg gcaagagtca gcccgacgga agggtcgtaa
     5161 ctgttgatat gataatagct taagcttgcg tgctctcacg agctgcgaaa tggtcgactt
     5221 tcagtcttgc catctgcggg tctggcccgt taaaatgtgc tgagaaggcg gagtggtacg
     5281 cgtgaggggt tgagcgaagc gttatcctgc ggcccctcgc gagtttacgg atgatggcgg
     5341 aaatgatgtt agaatccgcg cacaagggat tggccgttcg gcgaacccaa tccgtcacac
     5401 tttgtgcggg ataaaagagg tcgaggtata gttgctgtcc ctctgtcaag ggagctcata
     5461 attgcggtca aatcgggagg tcaagatgcg caagttcatg ttggcaagca cggtagtatg
     5521 gttcatcgtc ggccagacga tgggagcgag cacggtgacg atggagttgg agcttggggg
     5581 cagcaacaac ggtgccactt gcgccacccc gatctacttc acgccggggt ccaccgagga
     5641 tgggcagttc gctctcgatg aaaacggccg gctgacgtgg gcagtccgca ttcacgcgtc
     5701 ggggacgcac ttagatgcat cccaacagga agtcgccatt cgtggcgttg caaactttgt
     5761 cttcgatctc gagttgtatc atgattacgt ggacccggcc aacctggtca ccacggccac
     5821 gttcatgagc acagccaatc aggagatgac caattgcggc gttgagcaga actggtcaaa
     5881 cgtgcccgcc ggaatggctt atctcagcaa cgcagccttc gcagttgtct tcgacacaaa
     5941 cacgtccact tctgttggcc cgggccggat cgtcgattac tacagactga ctgatgggca
     6001 tccgggcgga ggtccacgaa tggatgtgcg ttgctacccg aaggctgcgg ccggcgaggg
     6061 caagctccag ggtgttggtg ccggctattc cctgtggaac aagagcagtc tcagcttgtt
     6121 gaccgtcggt ggaataggcc gtggcgattt cgccagcatc cccggcccgg ggcgaccgta
     6181 cggttatctc gctaacacgg aaacgaaggc gtcgggtgac ccgtcctacg gctggctggg
     6241 ctatggcccg gtcgtcgaag gtcagattga gggtctccag cccggcatca cctatatctt
     6301 gaggctcgtg cctggcggcg gaatcaacgt gctccgcggc ggcactcaga cccaaacctc
     6361 cgccgaggtc ttcgccatcg ccgctgacac caccgtcggt gatgacatta ccttcaccat
     6421 cgcgccggtc cagccgccgg tccagatcct cggctggtcg agcgtccgcg actgcggcgg
     6481 cgttttcagg gctatcccat tgaacccaac acgtagcgtc ggccaagtcc tcacaaccga
     6541 aacgcgaaaa gacggtctcc agatgattca aatcgatttt gatggcgaca taactggcag
     6601 gcacgtgcct ggacaaatct ccttgtcgaa c
//
LOCUS       DAOTPW010000290         6622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_10005_length_6622_cov_4.690879, whole genome shotgun sequence.
ACCESSION   DAOTPW010000290 DAOTPW010000000
VERSION     DAOTPW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10005_length_6622_cov_4.690879"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..938)
                     /locus_tag="P5316_19250"
     CDS             complement(78..938)
                     /locus_tag="P5316_19250"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HRR87152.1"
                     /translation="MRLSTREPGCRWVAVVWLAAIVGCTQPDIRPETALLERPYRYSD
                     RDWARVLQAYVRNGLVDYVGLAAHPEDLDRYYALLSVTGPASTPDQFPTRAAATAYWV
                     NAFNAMVLKVVLQHYPARSVYDVSLPRLETEYTFRVDRRIVTLAEVEQEMLRASDNDV
                     RTLFATSRAAVSTPLLGQEPIRAEALDQQLAAAAAKALDDPRILQIDSATHSIYVWQL
                     ILRRRNDFEDYWRSRRRASTGKLLDSLADMASPRRRQALRAAVGYSFRPIRFDGTLNA
                     VDQPRLQVVP"
     gene            1752..3260
                     /gene="rimO"
                     /locus_tag="P5316_19255"
     CDS             1752..3260
                     /gene="rimO"
                     /locus_tag="P5316_19255"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRR87153.1"
                     /translation="MTEPTTVALVSLGCVKNLVDSEKILGQLAERGAVIAADPADADI
                     IVINTCGFLQAARAEGHKVIAEMLRYRRSGRCRRIVVVGCLVQRDGHALLDAFPEIDA
                     LVSVHRRDDVPDAVLAGTTKPAAPPSQPRRRSGAKAGHRRQADLYLGAYHPHVALDTA
                     RLRLTPRHYAYLRISEGCNQKCSFCNIPAIRGPMRSKPPQVVLAEARELISDGAAELN
                     IIGQDTTAYGKDLHYRPGLAGLLRQLDKLDGLRWIRLLYAYPTAFTDDMIDAVAECSK
                     VVKYVDLPLQHINDRILKAMRRRVTRAQIEALLDKLRRRVPDVAIRTTLIAGFPGETE
                     EEFDELIHFVRQMRFAAVGVFAYSQEPDTPAGRFEAQLPEKVKQQRVEVIMKTQQEIA
                     FAHAAGLKGHVLEVLVDEKAARGRYIARHAGQAPDVDAVTYVLSDKVHPGRFVQVRCE
                     ASEGYDLIARPVDDILPNRKTGRTSRREAGRSRPTLESLLDPVGNTTKNHED"
     gene            3250..3861
                     /gene="pgsA"
                     /locus_tag="P5316_19260"
     CDS             3250..3861
                     /gene="pgsA"
                     /locus_tag="P5316_19260"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRR87154.1"
                     /translation="MKINLPNQITIGRLLLAIVFFICLEQYDAKAPKAWLMDFSLALF
                     VVAALTDVIDGYLARKHNQVTSFGRLIDPFVDKILVLGAYIFLAGDGFIDSAGRKVTD
                     VAMWMVVVILGRELLVTSLRGVTEAAGTSFGANIHGKAKMFLQSVTVGWILVTVGHPQ
                     SLAFAVGLRAPLIYLTVIITVLSGFSYLHAARHLLSETSRPTT"
     gene            3858..4370
                     /locus_tag="P5316_19265"
     CDS             3858..4370
                     /locus_tag="P5316_19265"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRR87155.1"
                     /translation="MMRKWAVTGLGLGFAPLAPGTFGSAGAILAGTAAWTICHKSATD
                     PAVLNVIWVLLTLLAGIGCVAWGPWAIDYFAARSRKPGDPGPVVIDEFAGQWISLVAL
                     PMGSLRQALTVLAVQFFLFRLFDVIKPPPARRLERLPAGWGILLDDLAAGIYANIVGQ
                     VIFRYFSLWS"
     gene            4405..5238
                     /locus_tag="P5316_19270"
     CDS             4405..5238
                     /locus_tag="P5316_19270"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRR87156.1"
                     /translation="MNDILRAVILGVVQGLTEFLPVSSTGHMILTMPLLGIHASDPDF
                     HFWNGAFDIFIQLGSILAVLIYCWRRLCRLTFNPSHTPWYEHFSVKLLIAFLPAIVVG
                     YLAHHFIEEKLKVPSVVACALIVGGILILVIQKAIKTPSINDAGAVSLPRAAIIGLIQ
                     CLSLIPGTSRSGATIMGALSVGMAPAAAAEFSFFLAIPTMFAAGLFSLYKHRHEISSD
                     QLMPLAVGFAVSFVVAWAVVAAFMRFIQTHRFTMFAIYRIALGVIVLAWVLGRSSIGS
                     P"
     gene            5509..5757
                     /locus_tag="P5316_19275"
     CDS             5509..5757
                     /locus_tag="P5316_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87157.1"
                     /translation="MVSSLGTPFSQAMSAFTDEQIASLARTYGSAIHPVWITDFQDRC
                     VYRNIPATRDKHPMNGALAFDILDHNGRVIGRLKTIVR"
     gene            6302..>6622
                     /locus_tag="P5316_19280"
     CDS             6302..>6622
                     /locus_tag="P5316_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87158.1"
                     /translation="MPEIAQNHPHIVVSKDKLRVEIDLRKTALEDVKPDRVKETLEKL
                     GVCITDEGAARIQDVAKLAAEAKRNKQDTLVLFSGKKPVAGQPAAFKPVEDRQQAGSE
                     KSGDA"
BASE COUNT         1283 a         2200 c         1857 g         1282 t
ORIGIN      
        1 ctgccggcta ggtggctcac gaagtcaaca gccgccgggt cgagctttgg catacaccgg
       61 tctcccccgt cttcccgtca cggcacaacc tgcaaccgcg gctgatccac ggcattcagt
      121 gtgccgtcga accggatggg gcggaatgaa tacccaaccg cggcccgcag agcctgtctc
      181 cgccttggcg aagccatgtc cgcaagcgag tcaagcagct tgcctgtcga cgcccgccgg
      241 cggctgcgcc agtaatcctc gaaatcattc cgacgtcgca ggatgagttg ccagacatag
      301 atcgaatgag tggccgaatc gatttgcagg atgcgcggat catccaaagc cttcgccgcc
      361 gccgccgcca attgttgatc gagcgcctca gcgcggatcg gctcctgacc cagaagcggc
      421 gtgctgaccg ccgcgcgact cgttgcaaac aacgtccgaa cgtcattatc gctggctcga
      481 agcatctcct gctcgacttc cgcaagggtc acgattcgcc gatccacgcg gaaggtgtat
      541 tccgtctcca gccgtggcaa cgacacgtcg tacacggatc gggcggggta gtgctgcagg
      601 accaccttca acaccatcgc attgaaagca ttaacccagt aagccgtcgc cgccgcacgc
      661 gtgggaaact gatccggcgt gctggcaggg ccggtcacgc tcagcagcgc gtagtaacgg
      721 tccagatcct cggggtgcgc cgccaacccg acgtaatcaa caaggccgtt tcgcacataa
      781 gcctgaagca cgcgggccca atcacggtcg gagtaccggt agggccgctc caacagtgct
      841 gtctccggac gaatgtccgg ttgcgtgcag ccgacgatcg cggccagcca gacgaccgcg
      901 acccatctgc aacccggctc ccttgtcgat agtctcacgc ttgggacact ccaacgaaca
      961 cgaccaccgt attctatcag gcaacgacac gccgtcgagt ggtatcacga tgcgcccatc
     1021 ttccggggta catgacagtc tgggcgggca tgccgaccgg tccaggttgg gcgatcaacc
     1081 ccgttcagcg ggtttgcccg ccaaaacggg cttgatgggg taacaccgtg aaggaggaat
     1141 cggccgaccg tgaaccggtc gtaaaacgac cgacctgttt gcccttcggg aaagaccgct
     1201 acagcggtct gctttcggct tgcgttcacg acaaccgggt cagccgccag gagtgccgcc
     1261 gcttctgtca cgggccccat cttccaggcc ccgtgacacg tcgccagtgt gtgccaccgg
     1321 tagcttgcct gcccgtgtcc ctcgcgcacg cgggcaagtc gcccgtggtg cttgcgaaat
     1381 gacctgccga ataccaagaa tcccgttccg cccacgccac cacaaccggc gaccgcgcca
     1441 ggacatcatc gcacccgaag accgcctcgc cagtttcagc gcaaaaaaac ccgaggccgt
     1501 gccgtccagc cggggtgaac cagacggact ttggggggtg cctcgggtac gatcatgtta
     1561 accggctccg cccaccgcct gcaagccccg tcagtgagac cgccgcagcg acagtacctg
     1621 agtcttggca ccacaaaaaa cgccgggccg gtaagcgtcg gccttttcca ccagcccctt
     1681 gacagatatg ctgtgggcat cgatcaagag gtcgcgcgag tcgcagccgc gttcttgctt
     1741 gagggccgtt tgtgactgag ccaacaacag ttgctcttgt ctcgctggga tgcgtcaaga
     1801 acctggtgga ttctgagaag atcctcggcc aattggcgga acgaggggcc gtcatagccg
     1861 ccgacccggc cgacgccgac attattgtga tcaacacctg cggtttcctc caagccgcgc
     1921 gggccgaagg ccacaaagtc atcgccgaga tgctgcgtta cagacggtcc ggccgttgcc
     1981 gccgcatcgt ggtggtcggc tgcctggtcc agcgcgacgg gcacgcgctg ctggacgcct
     2041 tccccgaaat cgacgccctc gtcagcgtcc accgccgaga cgacgtccct gacgcggtac
     2101 tggccggaac gaccaaaccg gcagcgcccc cgtcacaacc ccggcgcagg tcgggtgcca
     2161 aggccggcca tcgccgccag gccgatctct acctgggcgc gtaccacccc cacgttgcat
     2221 tggataccgc tcgcctccgc ctgacgcccc gccactatgc gtacctgcgg atcagcgagg
     2281 gctgcaacca gaagtgcagc ttctgcaaca tcccggccat ccgtggcccg atgcgatcaa
     2341 aaccccctca agtcgtcctt gccgaagccc gggaactgat ctccgacggt gcggcggaac
     2401 tcaacatcat cggccaggac accaccgctt acggaaaaga tctccactac cggccgggtc
     2461 tggccggctt gcttcggcaa ctcgataaac tcgacgggct gcgatggatt cgcctgctct
     2521 acgcttaccc cacggctttc accgacgaca tgatcgacgc cgttgcagaa tgctccaagg
     2581 tggtcaaata cgtcgacctg cctttgcagc acatcaacga tcgcatcctc aaggccatgc
     2641 gccgccgggt aacgcgagct cagatagagg cgcttctcga caagcttcgc cgtcgcgtcc
     2701 ccgatgtcgc catccgcaca accctcattg cgggttttcc aggcgagacc gaagaggaat
     2761 tcgacgagtt gatccacttt gtcaggcaga tgcgttttgc ggccgtgggg gtgttcgcct
     2821 attctcaaga gccggatacc ccggccggcc gattcgaagc acaactcccc gaaaaagtca
     2881 aacagcagcg cgttgaagta atcatgaaaa ctcagcagga gatcgccttc gcccacgccg
     2941 ccggcctcaa aggccacgtg ctcgaagtcc tcgtggatga gaaggccgcc agaggccggt
     3001 acatcgcccg ccatgcgggt caggcacccg atgtcgatgc ggtcacctat gtcctgtccg
     3061 acaaagtcca ccccggccgg tttgttcagg tccgttgcga agcctcggag ggttacgatt
     3121 tgattgcccg ccctgtcgac gatatcctgc cgaaccgaaa gaccggaaga acaagccgac
     3181 gtgaggcggg acgatccagg cccacgctgg aaagcctgtt ggatcccgtc ggaaacacca
     3241 cgaaaaacca tgaagattaa cctcccaaat cagatcacca tcggccggct gctgctggca
     3301 atcgtctttt ttatttgcct cgaacaatac gacgctaagg cccccaaggc ctggcttatg
     3361 gatttctccc tggctctctt cgtcgtcgcc gccttgaccg acgtgattga tggctacttg
     3421 gcccgcaagc ataatcaggt gacgtccttt ggtcgtctga tcgatccgtt cgtggacaag
     3481 atcctcgttc tcggagccta tatctttctg gccggcgacg gcttcatcga ctccgccggc
     3541 cgaaaagtca ccgatgtcgc catgtggatg gtagtcgtca tcctcggacg cgaactgttg
     3601 gtgaccagcc tccgcggcgt gaccgaggcc gctggaacgt ccttcggcgc aaatatccac
     3661 ggcaaagcca aaatgttcct gcagtccgtg accgtcgggt ggattctggt taccgtcggc
     3721 catccacaat cactggcgtt cgccgtcggc ctccgagcgc cgctcatcta cctgacggtc
     3781 atcatcaccg tgctctccgg cttctcctat ctccacgccg cccgccatct gctctccgag
     3841 acgtcgaggc cgaccacatg atgcgtaaat gggcggtaac cgggctgggg cttggctttg
     3901 ccccgctggc cccaggcact ttcgggtctg ccggagcaat cctggcgggc acggccgcat
     3961 ggacgatctg ccacaagtcc gcgaccgacc cggctgttct caacgtcatc tgggtactgc
     4021 tgacattgct ggccggcatc ggctgcgtcg cttggggccc ctgggccatc gattatttcg
     4081 ccgccagatc ccgcaaaccc ggcgacccgg gacccgtggt catcgacgag ttcgccggac
     4141 aatggatctc gctcgtcgcg ttgcccatgg gtagcctccg ccaggcactg acggtgctgg
     4201 ccgtccagtt ctttctcttt cgcctctttg acgtcatcaa gccgccgccg gcccgacgac
     4261 tggaaaggct gcccgcaggt tggggcattc tgctcgacga cttggccgcc ggcatctacg
     4321 ccaacatcgt cgggcaggtc atcttcaggt actttagtct atggagctga gcttccggcc
     4381 accggaggct ctagagaaac tggcatgaac gacattcttc gagccgtcat cctcggcgtc
     4441 gtccagggct tgaccgaatt cctgcccgtc tccagcacag ggcacatgat cctgacgatg
     4501 ccgctcttgg gtatccatgc aagcgacccg gatttccatt tctggaacgg ggcattcgac
     4561 atcttcatcc aactcggctc gatcctggcc gtcttgatct actgctggcg ccggctctgc
     4621 cgactgacat tcaatccctc ccacacgccc tggtacgaac atttctccgt caagctgctc
     4681 atagctttcc tgccggccat cgtcgttgga tacctcgcgc atcacttcat agaagagaaa
     4741 ctcaaggttc cttctgtcgt cgcctgcgcc ctcatcgtgg ggggaattct catacttgtc
     4801 attcagaagg caatcaaaac accgtcgatc aacgatgccg gcgctgtctc gctgccgcgg
     4861 gccgccatca tcgggctgat ccagtgcctg tctctgattc cgggtacttc tcgttcgggc
     4921 gccacgatca tgggtgccct gagtgtgggc atggctccgg ccgcagcggc agagttctcc
     4981 ttcttcctcg ctatccccac catgttcgcc gctgggttgt tctctctcta caagcatcgg
     5041 cacgagatca gcagtgacca actcatgccg ttggccgtcg gctttgcggt ctcatttgtc
     5101 gtcgcttggg ctgtggtggc ggctttcatg cgcttcatcc agacacacag gtttaccatg
     5161 ttcgccatct accggatcgc gctcggtgtc atcgtcttgg cgtgggtact cggccgatcc
     5221 tcaatcggct cgccataaca tcagtgaccg gccgaagttg cgacatcgcc aatacctgac
     5281 acccatttct attcacaggc gaaccaacca gccttaacgc tttgaccgca aagccgccga
     5341 tacttctaga gccggcttat agcctgtctc agtgggctgt gtcggctgcg cgggacaggc
     5401 ttccggcctg tcgataggct cctggttttc acgccaccgc atgcgagtat ggttagccgc
     5461 ggcccaaggc cgctggggca agccgcccct taattggagt cttgtcacat ggtatcctct
     5521 ctcggaacgc cgttcagtca ggccatgtca gccttcacgg atgagcagat cgcttcgctg
     5581 gcaagaacct acggcagcgc catccatccg gtctggatca ccgacttcca agaccgttgc
     5641 gtgtatcgca atatcccggc cacccgggac aaacacccca tgaacggtgc cctcgccttc
     5701 gacatcctgg accacaacgg aagagtaatt ggcaggctca agaccatcgt cagataatct
     5761 tttgctgccg cccctttacc gggctcatct attcggacgc atctcccaga gctgccggtt
     5821 ccggctcggt tggctgccag tgcggccgtc ggccaacggc aaaccccagc ctcaagcccc
     5881 cccttgtccc aaccgataga cggaaacggg acaaaccgac ttatgtctcc ctgttgaagg
     5941 gcggttcgcc ggcacaattg aagtgtgagt accgcttctt catgtaagcg gcctccaaca
     6001 tgtttgcacc gtgaggtgtc cggcgtgctc gaccgcctgc gggcaggcag acggcggttg
     6061 accggggtct ggacgtccgc cccgcggatg aaaggccgta cccgactgat cggcgaggct
     6121 cgcgggacgt gcagaacctt ggtgtgcctg cgtatgctat agtgagtaaa cgactgtgaa
     6181 atggagacgg acaagacata cttcggagat tgcgtgcttg agcccatgat tgctgcgttc
     6241 gcaaaggcgt tcagactcat cgccgacacg gacgcccccg ggtaggcccg caagaacgtc
     6301 aatgccggaa atcgcgcaga accatcccca cattgtcgta tccaaggaca aactgcgcgt
     6361 tgaaatcgac ctcaggaaaa ccgccctcga ggacgtcaaa cccgatcgcg tcaaggaaac
     6421 gctcgaaaaa ctgggcgtct gcattaccga tgaaggggcc gcccgcattc aggacgtcgc
     6481 caagctggcc gccgaggcca agcggaacaa gcaggatacc ctggtcctct tctcgggtaa
     6541 gaaaccggtc gcgggccaac ccgccgcgtt caagccggtc gaagaccgcc aacaagccgg
     6601 ctccgaaaag tccggcgatg cc
//
LOCUS       DAOTPW010000291         6602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_10048_length_6602_cov_5.974034, whole genome shotgun sequence.
ACCESSION   DAOTPW010000291 DAOTPW010000000
VERSION     DAOTPW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10048_length_6602_cov_5.974034"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..3419
                     /locus_tag="P5316_19285"
     CDS             78..3419
                     /locus_tag="P5316_19285"
                     /inference="COORDINATES: protein motif:HMM:NF024204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRR87159.1"
                     /translation="MTDATGLTIQAVQARISRLQLDTQLDEPTRAKAIEYCNLAIREL
                     QSADEWARKSADYQKIVQDAPVQLQTARDELRKAPESPAISSEAAGRTLEQLRQELAK
                     AETDLERSKQRLADLDKEPRRRLDRRTEIRLRREEISKETASVVEPQGPAALTMAFSV
                     YVQARAKARQAELAAYDRELASYEATSDLLSVQRDLAIRQVSQKEKLIRLWQEMIAQQ
                     RQEETARQVREAEEARRRADAETHPVARRIAALAEGNMTLLKRRQELGRKLEQVVQEY
                     NARKNLLDRMKRNAETVREKVQAAGVTKAMGQLLRKERTELPKVSTLRRGVELQHETI
                     AEVELELLDLRKQRDLLVDIPSLVSRDMLQFDPAFVEGRKWFEEELTKLYENERDYLQ
                     SLITDNGTYAGRLIDLQDVTESLIRETEEYLRFINQHILWLRSHEEMRFSSLLDAWDA
                     AKWLLKEAEWGDAARSLVRDARAHPLQVSLGVLVAVLLIAARPRFRRLLELSGERARQ
                     KLNTSFAPTAAALVCTLMLALSLSVVPFLLSWRLSAAVSTSETTKTIGLTLARVAAWL
                     LIVGGIRELLRPKGLAEAHLGWPPGALKVARWNLGWLLLIGMLMTFVIGFVEQWQVVA
                     DWKDSVSRLAFLIVLAAFAVFAARVFRPGSGIFAGRIARHRGGPMDRYRVLWYSCLVG
                     IPIVLAVLTVAGYYYTSLQLMARVYRTAWFALGMLVLRGLILRWLFLTHRRLAIAQAR
                     KRLAEKQAQQARDGSQPETDTAVVEEAEPELDIAALSQQTQHLFRAALGLALAVGLWL
                     IWADILPAINYLRNITLYSPRPETAITLVDLVLVVVTAVMTIIAAKNVPAFLEITVLS
                     HLPLKSGERYAIVTVVRYLILAVGTVVACSFAGISWTNVHWLIAAMTVGLGFGLQEIF
                     ANFVSGLILLFERPVRVGDTVTVGDVSGTVSRVQIRATTIITWDRKELIIPNKEFVTG
                     KVINWTLSDQSLRIVIPVGVAYGSDTAQVERILRAVAAEHPEVLRDPAPQAFFIGFGA
                     SSLDFELRVFIEGVDRLLSVKHALLNAIDKAFRAAGIEIAFPQQDIHIRSVKDVFPVV
                     QKPGDCTSAAKTTHVSGEIRRMPEA"
     gene            3420..3749
                     /locus_tag="P5316_19290"
     CDS             3420..3749
                     /locus_tag="P5316_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87160.1"
                     /translation="MNNADDIHAGAMCCLSLVALVSPFMLLFVRTSSLVKWLSLAALL
                     CSFAAAMAWALPRDADIPPLHMAFLFVLSGVAMVRHWFHASPPRTTGSEPQASADQHS
                     DIRSKGD"
     gene            complement(3997..4416)
                     /locus_tag="P5316_19295"
     CDS             complement(3997..4416)
                     /locus_tag="P5316_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRR87161.1"
                     /translation="MTHQDSISIRTSGHRDMHDITEQVSRIVRESGIRTGIVHLFNVG
                     STGALGTIEFEPGLRHDLPELLDRLIPPSRDYGHEAAWNDGNGHSHLQASWLGPDLTV
                     PVSNGRLALGTWQQIFHLECDIKPRQRQIVVTVTGEG"
     gene            complement(4429..>6602)
                     /locus_tag="P5316_19300"
     CDS             complement(4429..>6602)
                     /locus_tag="P5316_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87162.1"
                     /translation="PHHQTGGFTACSDRRRQGEPPQAHGLLERCPNCGRYHVNNADEM
                     VNEERPMEASGFLLSPTQVLTADPLVHPRFVKDMVVRSGSVSVKAKISGFAMQQNAIF
                     LELSEPLPGTRPLVFDAAAKGPYLAVTCKNLNGEWAVNVAAMPLSVTSTVSNRRFCAA
                     PSQCLIVTREGTPVAVCMKDEVSADETWKGSPQQWDAMSAQDMEAALKALEDRCSRCL
                     LRVALSFRSPKSQGQERYRYSYSYYGEDSEDADATERNVMGLLLDEKRVMVLAQLEAK
                     VTARLERVVVHPAEGKPVPAKFDCSLTDYGCLLVTLETPLEGAVRTSQRPILEYRDQV
                     LLSADVSVKGETRIGYYGHQRIAAFATGWKQRVYPESAGDMRGVFLFDRDLLLVTLPI
                     ARRKKVTLRQEYSYSSGEVQMTPAMYVQEALADLPNQIDLNNVPLSEEEENRLAWLGV
                     ELQALNRELARANNVSDLTGDGRSGALVTFVHPDSPAAKAGIEAGYILIRLHVEGHPK
                     PLEVRLSDDRYGGDESFPWDRLDEIPAEYFEQLPKPWPAAENSFTRALTDLGFGKKYT
                     AEFFHEGKVINKEFEVVVSPPHFDSAPRFKSESLGMTVRNLTYEVRRYFQKKDDDPGV
                     IVSKIEPGGKASVAGMIPYEIITHVNDNAVTNVKDFERLISDQKQLRLSVNRKTQGRI
                     VKIDMTTPAGGKGLADKLRSSLRRPRAETAPTEEATTQSRE"
BASE COUNT         1193 a         2049 c         2056 g         1304 t
ORIGIN      
        1 gatcggccgc cgatcgcgca aaactgccac cggagttcgc ttgttttggg gctagtgcgc
       61 cgaccaccga gccggcggtg acggacgcga ccgggctgac cattcaggcg gttcaggccc
      121 ggatcagccg cctgcaactg gacacgcaat tggacgagcc cacgcgggcc aaggcgatcg
      181 agtactgcaa cctggcgatc agagagctgc aatccgcgga cgaatgggca cgcaagagcg
      241 ctgattatca gaagatcgtc caggatgccc ccgtgcaact ccagaccgct cgggacgaac
      301 tgaggaaagc tccggagtca cccgccatct cgtccgaggc agccgggagg actctggaac
      361 agctcagaca ggagcttgcc aaggccgaaa cggacctgga aaggagcaag cagcgtttgg
      421 ccgatctcga caaagaaccc agacgcaggc tcgaccgccg caccgaaatc aggcttcgcc
      481 gggaagagat cagcaaagaa acggcctccg tggtcgagcc ccagggaccg gcggcgctga
      541 ccatggcctt cagcgtctac gtccaggccc gtgccaaggc ccgacaggcc gagctggccg
      601 catacgacag agagctggcc agctatgagg ccacaagcga cctgctctcg gttcagcgag
      661 acctggccat ccgccaggtg tctcagaaag agaaactgat ccggctctgg caggagatga
      721 ttgcccaaca acggcaggag gaaaccgcac ggcaggtgcg cgaagcggaa gaagccaggc
      781 ggcgagcgga tgccgaaact catccggtgg ccagacgaat cgcggccttg gccgagggaa
      841 acatgacgct cctcaagcga cgacaggagc ttggaagaaa gcttgaacag gtggttcaag
      901 aatacaacgc ccggaagaat ctgctggacc ggatgaagcg aaacgccgag acggtgcgcg
      961 aaaaggttca ggccgcgggc gtgaccaagg ccatgggaca attgcttcga aaggagcgaa
     1021 ccgagctccc caaggtaagc actctccggc gaggggtcga gttgcagcac gagacgatcg
     1081 ccgaagtcga gctggagctg cttgacttgc gaaaacagag ggacttgctg gtcgacattc
     1141 cctctctggt ttcaagagac atgctccagt ttgacccggc ctttgtcgaa ggtcgaaaat
     1201 ggtttgagga ggaattgacg aagctttacg agaatgagcg ggattacctc cagtcgctga
     1261 tcaccgacaa cggcacctac gccggtcggt tgatcgacct ccaggacgtc accgagtcgc
     1321 tgattaggga gaccgaagag tatctgcgtt tcatcaatca gcatattctg tggttgcgca
     1381 gccacgagga gatgcgtttt tcaagcctgc tcgatgcctg ggacgcggcg aaatggctgc
     1441 tgaaggaggc ggaatggggc gacgcggcga gatccctggt ccgggacgcg cgggctcatc
     1501 cgctccaggt gagtctcggc gtgctggtgg cggtgctgtt gatcgcagcc cgcccccggt
     1561 ttcgacggct gcttgaactg agcggtgaga gggctcgaca gaagctgaac acctcgttcg
     1621 ccccgacggc ggcggcgttg gtatgtactc tcatgttggc tttgtcatta tcggttgtgc
     1681 ccttcttgct gtcctggcgg ctcagcgcgg cggtgtcgac ctctgaaacc accaagacga
     1741 tcggcctgac cttggcccgg gtggccgcat ggctgctgat cgtcggcggc atccgcgagc
     1801 tgttgcggcc caaagggttg gccgaggctc acctcggttg gcccccgggc gccctgaagg
     1861 tggcccggtg gaacctggga tggctgctgc tgatcggcat gttgatgacc ttcgttatcg
     1921 gtttcgtcga acaatggcag gtggtcgccg actggaagga ttccgtgtcc cggcttgcct
     1981 tcctgatcgt cttggctgcg ttcgccgtct ttgccgccag ggtgttcaga ccgggcagcg
     2041 gcatcttcgc cggccgcatc gcccgccatc gcggtgggcc gatggatcgc taccgggttc
     2101 tctggtactc atgtctggtg ggtatcccga tcgtcttggc agtcctcacc gtcgccggat
     2161 actactacac gtcccttcag cttatggccc gggtctaccg aacggcgtgg ttcgcgcttg
     2221 gcatgctggt tcttcgcggc ttgatacttc gatggctgtt cctgacccac cgccggctgg
     2281 cgattgccca agcccgcaag cgtctggccg agaaacaggc gcagcaggcc cgcgacggca
     2341 gccaacccga aactgatacg gccgttgtgg aggaagccga gccggaactc gacattgcag
     2401 ccctcagcca gcagactcag cacctgttcc gcgccgcact ggggttggct ctggccgtcg
     2461 gcctttggct gatctgggcc gatattctcc cggcgatcaa ctatctgcgg aacatcacgc
     2521 tgtactcgcc tcggcccgag acggccatca cgctggtcga tctggtcctg gtcgtcgtga
     2581 ccgcggttat gacgatcatc gccgccaaga acgtgcccgc cttcctggaa atcaccgtat
     2641 tatcccatct gccgctgaag tccggcgagc gatacgcaat cgtcaccgtc gttcggtatc
     2701 tgatcctggc ggtgggcacc gtggtggctt gcagcttcgc gggcatcagt tggaccaacg
     2761 tgcattggct gatcgccgcg atgaccgtcg gtctgggttt cggtttgcag gagatcttcg
     2821 ccaacttcgt ctccggcctg atactgctct ttgagcggcc ggttcgcgtc ggcgacaccg
     2881 tgaccgtcgg cgacgtcagc ggaacagtca gtcgcgttca gatccgggcg acgaccatca
     2941 tcacctggga ccgcaaggag ttgatcattc ccaacaagga gttcgtcacc ggcaaggtga
     3001 tcaattggac gttgtccgac cagtcgctgc ggatcgtcat tccggtgggc gtggcgtacg
     3061 gctcggacac tgctcaggtg gaacggatcc tgcgagcggt cgccgcggag catcccgagg
     3121 tgctcaggga ccccgccccg caggccttct tcatcggctt tggagccagc tcgcttgatt
     3181 tcgagttgcg ggtgttcatc gagggtgtcg accgcctgct cagcgtaaag catgccctcc
     3241 tcaacgccat cgacaaggct tttcgcgccg ccggcatcga gatcgccttc ccgcaacagg
     3301 acattcacat ccgctccgtc aaggacgtct ttccggtggt gcagaaaccg ggcgactgca
     3361 cgtctgctgc gaagaccacg catgtcagcg gagaaatcag gaggatgccg gaggcttgaa
     3421 tgaacaacgc agatgatatc cacgcgggag ccatgtgctg cctttcactg gtggccctag
     3481 tgagcccatt tatgctgctg ttcgttcgca cctcttcgct ggtcaaatgg ttgagcctgg
     3541 cagcgcttct gtgttccttc gcggcggcca tggcttgggc actcccgcgt gacgccgaca
     3601 ttcctcccct gcacatggct tttcttttcg tcttgtccgg ggtcgcgatg gttcggcact
     3661 ggtttcatgc atcaccccct cgaacgacag gatcagaacc gcaagcatcc gcagaccaac
     3721 attcggacat ccgcagcaag ggagactgaa tagaaccttt cagccggaca tctgtgtggc
     3781 ttgcgcgcat gtttctggat ggcagtgcgc ttgggttgcg gccggcacaa aggcggtccg
     3841 ccttaggaac ggaacgcgga gtttgacaga tgctatgtgc tcgggccgcc atctcgctgg
     3901 cgaatcacgg cagtgccgac gggtgagcgt gtcacgatgg tcgagcggcg cagggtgtgc
     3961 gccgacgaat gtcgtccttg actcatcctt cagctttcat ccttccccag tcaccgtcac
     4021 cacgatctgg cgctggcgcg gcttgatgtc gcactcaagg tgaaagatct gctgccaggt
     4081 gcccagcgcg agccggccgt tgctgaccgg cacggtcaga tccggaccaa gccaactggc
     4141 ctgcaggtgc gagtgtccgt tgccgtcatt ccacgctgcc tcatggccgt aatcgcgact
     4201 cggcggaatc agccggtcga gcagctcggg caagtcgtgc cggaggcccg gctcaaactc
     4261 gatcgtgccc agcgcaccgg tgctgcccac gttgaacagg tggacgatcc ccgtccggat
     4321 gcccgactcg cgcacgattc gcgagacctg ctcggtgata tcgtgcatgt cccgatgacc
     4381 cgacgtgcgg atgctgatgc tgtcctgatg cgtcaccgtt cggtcgcctc actcgcgcga
     4441 ctgcgtcgtc gcctcttcgg tcggcgcggt ctccgctctc ggccgtcgca gcgatgaccg
     4501 cagcttgtcc gccaaccctt tgccgcccgc cggcgtcgtc atgtcgatct tgacgatccg
     4561 gccctgtgtt ttccggttga ccgacagacg cagttgcttc tggtcggaaa tgagtctctc
     4621 gaagtccttg acattcgtga ctgcgttgtc gttcacgtgg gtaatgatct cgtagggaat
     4681 catccccgcc accgaggcct tgccgccggg ttcgatcttc gacacgatca cgcccgggtc
     4741 atcgtctttt ttctgaaagt agcggcgaac ctcataggtc aggttgcgca ccgtcattcc
     4801 cagtgattcc gacttgaacc ggggcgccga gtcaaaatga ggagggctga ccaccacctc
     4861 gaattccttg ttgatgactt tgccttcgtg aaagaactcg gcggtgtatt tcttgccgaa
     4921 tccgaggtcg gtcagcgccc gggtgaagct gttctcagcg gccggccacg gtttgggcaa
     4981 ctgctcgaag tattcggccg ggatctcgtc gaggcggtcc cacgggaaag attcgtcccc
     5041 gccgtagcgg tcgtcactca gtcgcacctc gagcggcttg gggtgcccct cgacgtgaag
     5101 ccggatcaga atgtatcctg cctcgatgcc ggccttcgct gcgggcgaat cgggatgcac
     5161 gaaggtgacc agcgccccgc tccggccgtc cccggtgagg tcggatacgt tgttggcccg
     5221 tgccagctcc cggttgaggg cctgcaactc cacccccagc catgccagcc ggttttcctc
     5281 ctcctcgctc aggggaacat tgttcaaatc gatctggttg gggagatcgg caagggcctc
     5341 ctgaacgtac atcgccggcg tcatctgcac ctcacctgat gagtatgagt attcctgtcg
     5401 caaggtcacc ttcttacgcc tggcgatcgg gagagtcacc agcaacagat ccctgtcgaa
     5461 aagaaagacg ccgcgcatgt cgcccgccga ctcgggatac acccgctgtt tccagccggt
     5521 cgcgaaagcg gcgatgcgct gatggccgta gtagccgatg cgcgtctcgc ctttcacgga
     5581 gacgtcggcg gatagcagca cctggtctcg gtattcaaga atcgggcgtt gcgaggtgcg
     5641 aacggcgccc tccagcggcg tttccagggt aaccagcaga cagccgtaat cggtcaacga
     5701 gcagtcgaac ttggccggca ccggtttgcc ttccgcaggg tgtaccacca cgcgttcgag
     5761 cctcgcggtg accttggcct caagctgagc aagcaccatc acgcgtttct cgtcgaggag
     5821 cagccccatc acgttgcgct ccgtggcgtc cgcatcctcg ctgtcctcac cgtagtagga
     5881 atacgagtag cgatatcgct cctgcccctg cgatttcggg ctgcggaagc tcagcgccac
     5941 gcgcagcagg catcggctgc agcggtcctc gagcgccttg agtgccgcct ccatgtcctg
     6001 ggcggacatg gcgtcccact gctgcggcga gcccttccac gtctcgtcgg ccgacacctc
     6061 gtccttcatg cagacggcga ccggcgtgcc ttccctcgtc acaatgaggc actgggacgg
     6121 ggcggcgcag aatcgccgat tcgagaccgt cgaggtgacg ctcaggggca tcgcggcgac
     6181 gttgaccgcc cattcgccgt tcagattctt gcaagtgacc gccaggtacg gccctttcgc
     6241 ggcggcgtca aaaaccaacg gcctggtccc cggcagcggc tcactcagtt ccagaaagat
     6301 ggcattctgc tgcatggcga agccggagat cttcgccttg accgagacgc tgccggaccg
     6361 gaccaccatg tctttcacga atcgcgggtg cacaagcggg tcggcggtca gcacttgtgt
     6421 cggcgacagc aggaaccccg aggcttccat cggacgctcc tcattaacca tctcgtcggc
     6481 gttgttcaca tgatatctgc cgcagttggg gcaccgttca aggaggccgt gggcttgagg
     6541 cggctcgccc tgtcggcgcc ggtctgaaca tgctgtaaat ccgccggttt gatgatgcgg
     6601 tg
//
LOCUS       DAOTPW010000292         6523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_10195_length_6523_cov_5.380489, whole genome shotgun sequence.
ACCESSION   DAOTPW010000292 DAOTPW010000000
VERSION     DAOTPW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10195_length_6523_cov_5.380489"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            763..1437
                     /locus_tag="P5316_19305"
     CDS             763..1437
                     /locus_tag="P5316_19305"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR87163.1"
                     /translation="MDRQKTKEAGVSKRQVAYERLRRILILQQIPEGVRLRESEWTKR
                     LNVNRSALREAFARLEAEGLIVTGEKKGYFVPVLVMEDIMEVVVVRIALEGAAIELIC
                     EAGMNTPEHLKPLQDACDLMEQLINEDYHLSVAETDRRFHAALIEASRNKRLVIAYQH
                     APLPVVHPDIIDGQQWANRVRQTHEEHSAILAAILAGQVAEAKDLLRAHLTSSWGTVR
                     LAGKTV"
     gene            complement(1618..1690)
                     /locus_tag="P5316_19310"
     tRNA            complement(1618..1690)
                     /locus_tag="P5316_19310"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1655..1657),aa:Gln,seq:ctg)
     gene            2117..2938
                     /locus_tag="P5316_19315"
     CDS             2117..2938
                     /locus_tag="P5316_19315"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR87164.1"
                     /translation="MWPKGINGRSFPKGTSWADAARRAKAAGFDSIEPTLDIQGELTP
                     VSTEAECRRISDAIRGSGLEVAALACGFLWEKPYTSADPLVREEAVSLTKAALQRASW
                     IGAPILLVLPGLVSHPRSPATMVTAYAEALRRADTALTELSYDAESFGVTIAIENACN
                     QFLVSPIEMCDFLDRVNSPWVGAYFDVGSVLRYGFPQDWIDSLGRRIVGVHLRDYKLA
                     KDPEPGFCALGEGDVDWPAVIHALRRHRYQGPLVYEGKEDLTTAALFIERLMHGP"
     gene            3680..4603
                     /locus_tag="P5316_19320"
     CDS             3680..4603
                     /locus_tag="P5316_19320"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRR87165.1"
                     /translation="MDLAIKLLIGFLSGILGGLLGIGGSIIMIPAMTMVFGLDQHLYQ
                     GAAMIMNFFVALPAAMQHFRAKAVLRPVIQVTIPTAVVGVLAGVWISKGSWFAGANEI
                     YLSRLFGVFLLYTAGYNVYRLMSSYRLPEIDETTARTIPKWKSAVSVGLPMGLIGGLL
                     GVGGGIVAVPLQQLILKMPLRRAIANSAVTIVPLSIIGAVYKNVANAQAGVDVRASIL
                     LALCVIPTAILGGFLGGRLAHTAPRQALRFLVILLMCYAGVKMVSRRLPAETAVPEST
                     ATVRVSLASPDDRRPISCTTTKGSTRTAAEK"
     gene            complement(4578..5132)
                     /locus_tag="P5316_19325"
     CDS             complement(4578..5132)
                     /locus_tag="P5316_19325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR87166.1"
                     /translation="MDYTAWSAMYTTPIRVRNGLYCLRDFDPSAAPVVAGWARDAAEL
                     FWLAPSTPPPLTAAKVLGWQNADVCALLFQREASDELLGYLELNLMPAQTAHLWMGHC
                     VIAPSHRGQGLGRMMVSMAMEHAFTHRKAESVSLIVFPDNVAAIECYKRVGFAAAGEQ
                     LKDFRTTGRTHRMLRMLISRQQYA"
     gene            5191..5856
                     /locus_tag="P5316_19330"
     CDS             5191..5856
                     /locus_tag="P5316_19330"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR87167.1"
                     /translation="MTVDCYLGYVVRRETWRVGGQTFHLIWPADIDGLLDLPDVQERF
                     SKDQYMPYWAQPWPAGVLLAEAILAGEDGQGRQAVELGCGIGLVSLAAARMGWSITAS
                     DYDEHAIAFARLNAANNNICLAAARMIDFRLPPESSAYERVFGADLTYERRNAEPLAR
                     WLASALKAGGEAWLSDPNRSAGDEFPSHARALGLKVDVRAVESTSPVGLLHRGRIWRV
                     CRP"
     gene            6127..>6523
                     /locus_tag="P5316_19335"
     CDS             6127..>6523
                     /locus_tag="P5316_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR87168.1"
                     /translation="MRVLVIEDEPDLLEALAQALREAGYAVDEASDGRNGLFKAAGTD
                     YDAIVLDLMLPGMSGWDLLRELRKSKSTPVLILTARDTLPDRVRGLDAGGDDYLTKPF
                     ELPELLARLRALIRRGAGQATATIDIGDVA"
BASE COUNT         1246 a         1955 c         1988 g         1334 t
ORIGIN      
        1 agcaccgcga caccaaacac ggttgctttt ctcctcataa taagcgttcc tcctcttttc
       61 ctcgggcgca tgcgcgcctt ccccaaaaac attacaactg gatttcagac ggcagaactc
      121 ttccgcctcc taatcctgaa tccgcacgtt cgaggtcgcc cttccgtgag attcccggtc
      181 gccgggtcta cgccgtttcg gaaatctccc tcgctgccaa gccgcaaagc gagatgtatg
      241 tgccacagcg actggcagac gcgatctctc ctcgtagact atacgctgac gggatagcga
      301 tgtcaacagc gatttagccc gattcgtcaa cacgatttca gggagttggt ccacggatca
      361 tatcacgccg tgtcagaacg gcgcctgtcg accagccgat ccggtccgcg cagcaattcc
      421 actcgagcga ctggctggac ttggaggtgg cggttccgca acgtcctgtt ggcgacgctc
      481 gattcggagc tgccgccgag ccctaagaca ccctggaaac actgcttacg gttgtgagcg
      541 atgaggctct cccacacggc gaagagtgat gctttccggt ggccagtgcg ccccggcagt
      601 agagcaaggg cgtgatactc gaatcgcttc gcggatggca ccgtcgaccg acccggctgc
      661 gattgtggca aaaaaacatt gccgcagcga cgcttttctc gtagactgaa aatcgctgaa
      721 aacataacgg gtcgcctgcg gacagaaatg aggtgtagcc ccgtggacag gcagaaaacc
      781 aaagaggcgg gtgtctcaaa gcggcaggtg gcgtacgaac gccttcggag gattctcatt
      841 ctacagcaga tacccgaagg agtgagactg cgcgaatccg aatggaccaa gcgactgaat
      901 gtcaatcgct ctgccctgcg cgaggcgttc gcccgcttgg aggcggaagg cctgatcgtc
      961 acaggcgaga agaagggcta cttcgtgccc gttttggtca tggaagacat catggaggtg
     1021 gtcgtcgtac gcattgccct cgagggagcc gccattgagc tcatctgtga ggccggaatg
     1081 aacacgcccg agcacctcaa gccgttgcag gatgcctgcg acctcatgga gcagttgatc
     1141 aacgaagact accacctgag cgttgccgag actgataggc gatttcatgc cgcgctcatt
     1201 gaggcctcgc ggaacaagcg attggtgatt gcatatcagc atgcacccct gccggtcgtg
     1261 catcccgaca tcatcgacgg gcagcaatgg gcaaatcgcg tcaggcagac gcatgaagag
     1321 cacagcgcca tcctggcggc cattctggcc ggccaagtgg ccgaggcaaa ggatctgctg
     1381 cgagcccatc tcacttccag ttggggtacc gtcaggctgg cgggcaagac ggtctgatgt
     1441 gggtcgccca acccccgcag agaccagtcg ttggcgaaga ttcgctgagt tttttgggga
     1501 gccgatccgg ggcaagccgt cctgctccgt tcggatccgc cgataccggc ctgcctccgc
     1561 ctcgaccgtc ggtcggattc aaggctaagc aaatgatgta caaagcctta tgtcaatctg
     1621 gcccgctagg actcgaacct agaaccttct gatccagagt cagacgtgct accagttgca
     1681 ccacgggcca agacgttttg cgcggcccga caggctcgct gacgatgcca aaccatgccg
     1741 gcatctccct gtcgacgcgc cactgcaatt gtgtgacggc ggagttatcc tgtcaagccg
     1801 ctgcctccag ttgcaggagg gcaatacgct ctcatagtct tatatctcct gcggccgggc
     1861 tgaatggaac cctgcggcca gacatccgtg cggcttgcgc gcatgtttcc ggatcgcggt
     1921 acgcttgggt tgcggcccgc aaaaaggcgg tccgccttag ggcaagaatc cgcgacttgg
     1981 ggtttgcggt ccttcggcga atgacaggga gcagcaacca ggcgttttct tcgccgacgc
     2041 aggcagccct gcgactgatt cggcttcgtg ggtcgcgagg gcgggacatg acggacactg
     2101 acggaggtgc cgcaagatgt ggcccaaggg catcaatggc cggagctttc ccaaggggac
     2161 gtcgtgggct gacgctgctc gcagggcaaa ggctgccggt ttcgactcca tcgagccgac
     2221 gttggacatc caaggagaac tgacacccgt atccaccgag gccgagtgcc ggcgcatctc
     2281 agatgcgatt cgcgggtcgg gactggaagt tgccgccctc gcctgcgggt tcttatggga
     2341 gaaaccgtac acctcagcgg atcccctcgt tcgcgaggag gccgtctcgt tgacgaaggc
     2401 cgcgctgcag cgagcaagct ggatcggagc gccgattctt cttgtgctgc ccggactggt
     2461 ctcgcatccg cgcagtcctg ccacaatggt tacagcttat gccgaagctc tgcgacgggc
     2521 cgacacggcc ttgaccgaac taagctacga tgccgaatcg tttggggtga cgatcgctat
     2581 cgagaatgcc tgcaaccaat tcctcgtttc gccgatcgaa atgtgcgact ttctcgaccg
     2641 agtcaattca ccgtgggtag gggcgtactt cgatgtgggc agcgtgctgc gatacggttt
     2701 cccgcaagat tggatcgact cgttgggcag gcggatcgtg ggagtgcatc tcagggatta
     2761 caaactggcg aaggaccctg aaccgggttt ttgtgccctc ggcgagggtg acgttgactg
     2821 gcccgcggtg atacacgccc tgcgccgtca ccgctaccag ggtcccttgg tctacgaagg
     2881 gaaagaagac ctcaccactg cggcgctctt cattgaacga ttgatgcacg gaccctgatg
     2941 tagcacaacg cggcaaaaca ccgtgagccg ctaccactca catctgcctc ggctgcacgt
     3001 atcccctccg ccgtcgtcac gcaggcgccg gtgagacgaa tcggcaacgc ggccgttggc
     3061 atcaaccccg cagcaggtcg gctcagggca tggaaaacga gttcgacagg gcgttattat
     3121 acggccgcga tttcggccct ggccccgtga tgcccggctt ctcggcatgg aaaacaagtt
     3181 ccgctgggtg ttattcatac gctggcggtg gggctaaaga gtgcaggcga cacaaaatgc
     3241 gggcccaact tcttgctaac tccgttattg ccgggcacat aggcgattcg agtcgcacgc
     3301 ccgacaggca cgccggcaac gcatgcgagc ttgccggccg ggcctcagct ggccgatcgc
     3361 caagagtatt cgctcgcgtc gcgaaaaaag tcttgacgcg gtgccgccga acgcttagat
     3421 tatgggatgt tggggttgat gagggagaga gttgtgggag agagagtgtt ccgcggcagg
     3481 agcattctct ctttcttttt ttgcgccgcc cgcgcccctc gagctgcatt cattcctgac
     3541 gcgtccggtc cgcaatagca aggtccttgc tcgtcccacc taagaagccc tcttgccgtc
     3601 cgatgtttcc ctcgactcac ctgctcggta gaatcgcacc tcctgacact ctcgtcgagg
     3661 cgggcgggct gaatcaaaga tggatcttgc gatcaagctg ctaatcgggt tcctttccgg
     3721 catcctggga ggactgctgg gcatcggcgg cagcatcatc atgatccccg ccatgacgat
     3781 ggttttcggc ttggatcagc atctctatca gggggccgcg atgatcatga atttcttcgt
     3841 cgctctcccc gctgcaatgc agcatttccg tgccaaggca gtcctgcgac cggtgattca
     3901 ggtgacgatc cccacggccg tggtgggcgt actggccggc gtgtggatca gcaaaggctc
     3961 gtggttcgcg ggtgccaatg agatctattt gtcccggctc ttcggtgtct ttctcctgta
     4021 tacggcgggc tacaatgtct atcgactcat gtcgtcctat cgcttgccgg aaatcgacga
     4081 aacgaccgcg aggaccattc cgaaatggaa gtcggccgtt tccgtcggcc tgccaatggg
     4141 attgatcggc ggcctgctgg gcgtcggcgg gggtatcgtt gccgtgccgc ttcagcagtt
     4201 gatcctgaaa atgccgctcc ggcgagccat tgccaactct gcggttacca tcgtaccctt
     4261 gagcatcatc ggggccgtct ataagaacgt cgcaaatgct caggccggcg tggatgtgcg
     4321 agcttctatt ctgctggcgt tgtgtgtcat tccaactgcg attctgggcg gcttcctcgg
     4381 cggacggctc gcccacaccg cgcctcgcca ggcacttcgc tttcttgtca tcctgttgat
     4441 gtgctatgcc ggagtcaaga tggtttctcg ccggttgccg gcggaaaccg ctgtacccga
     4501 gtcgaccgct acagtgcgag tctcgctggc ctcgccggat gatcgccgcc cgatctcctg
     4561 cacgacgacc aaggggtcta cgcgtactgc tgccgagaaa taagcatccg cagcatgcga
     4621 tgagtccgac cggtggtgcg gaagtccttg agctgctctc ccgcggccgc gaagccgacg
     4681 cgtttgtaac attcgatggc ggctacgttg tccggaaaca caatcaggct gacgctttca
     4741 gccttgcgat gcgtgaacgc atgttccatc gccatgctta ccatcatgcg acccaggccc
     4801 tgaccccggt ggcttggggc aatgacacag tgccccatcc acaggtgggc ggtctgagcg
     4861 ggcatgaggt tgagttcgag atatcccagc aactcgtcgg acgcctcccg ttgaaagagc
     4921 aaggcgcaca cgtctgcatt ctgccagccc agaacttttg ccgcagtcag cggcggtgga
     4981 gtgctcggtg ccagccagaa taattcggcg gcatccctgg cccaccccgc aaccaccggc
     5041 gctgctgacg ggtcgaagtc ccgcaggcag tacaatccgt tgcgcacgcg tatcggcgtc
     5101 gtgtacatgg ctgaccacgc agtataatcc attctggtcc cgaatacgca cgcgtatcgc
     5161 ttgccgtttc gccaccggcg caggatttcc atgaccgttg attgttatct gggttatgtg
     5221 gttcgcagag agacttggcg ggtgggggga cagaccttcc acttgatttg gccggcggac
     5281 atcgacggtc ttctcgacct tcctgatgtg caggagcggt tctccaaaga tcaatacatg
     5341 ccttattggg cgcagccgtg gccggcgggc gtgcttctgg ctgaggctat actggcgggt
     5401 gaggatggcc aaggccgaca ggcggtcgaa ttgggctgcg gcatcggctt ggtcagtctt
     5461 gcggcggcca gaatgggctg gtcgataacc gccagcgact acgatgagca cgccattgcg
     5521 tttgcccggt taaatgccgc aaacaacaac atctgccttg ctgcggcgag gatgattgat
     5581 ttccgactgc cgcccgagtc gtcagcctac gaacgcgtgt tcggcgctga tctgacctac
     5641 gaacgacgca acgcagagcc cttggcccga tggctggctt ccgccctgaa agccgggggc
     5701 gaggcgtggc tgagcgaccc taatcgatcc gctggcgacg aattcccgtc gcatgcccgg
     5761 gccctcgggc tgaaggtcga cgtgagagcc gtggaatcca cctcgcctgt cggcttgctc
     5821 caccgaggtc gaatctggag agtctgtcga ccgtaatcgc tgctgcctgg cagcttctgc
     5881 aggacgtgag ccgcccccac acaaccggct ctctggttaa gctccgctat tgggaccatc
     5941 ctgctcagag gcagccaggg tatttcattg cccgcaggaa cgccgaatcg gcctcgacgg
     6001 gtacgacagg caatggcaaa gctgctgcgc cgtaacgttc ccgtttggcc ttcacgtgct
     6061 cttaaggtta cgttcgctat gctccgtctg acgaaagact ctgcaacgga actcctgagg
     6121 catcgcatgc gagtactggt gattgaagac gaacccgacc tgttggaagc gctggcacag
     6181 gcacttcgcg aggctggata cgcggtcgat gaggcctcag acggccgcaa tggtctgttc
     6241 aaggcggccg ggacggatta cgacgcgatt gtgctcgacc tgatgctgcc gggcatgagc
     6301 gggtgggacc tgctgcggga gctgcggaag agcaagtcga cacccgtgct catcctgact
     6361 gcccgcgata cgcttcctga ccgggttagg ggcctcgacg ccggaggcga tgactacctt
     6421 accaagccgt tcgagctgcc tgagcttctg gctcgtctcc gggcattgat tcgccgcggc
     6481 gcgggccagg caaccgcgac gatcgacatc ggagacgtgg cgg
//
LOCUS       DAOTPW010000293         6492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_10257_length_6492_cov_5.961939, whole genome shotgun sequence.
ACCESSION   DAOTPW010000293 DAOTPW010000000
VERSION     DAOTPW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10257_length_6492_cov_5.961939"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1063)
                     /locus_tag="P5316_19340"
     CDS             complement(20..1063)
                     /locus_tag="P5316_19340"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRR87169.1"
                     /translation="MKQAQASYRPSSQALGGVRRFLFPAMLSIAFGCASTGRDALSAW
                     PLHVIDNSSAGADGVRLADVNGDRLPDVAAAWEEGGLIRVYLHPGTRAARRPWPAVTV
                     GQVSSPEDAVFADLDGDGAFDVVSCCEGKEKTVFLHWAPRERSRYLDPTAWKTEGLPA
                     SKGVCQWMFCLPMQVDGCNGIDLVVGGKNDGAGIGWFEAPNEARDLNAWKWHPLYDAG
                     WIMSLIAEDMDGDGDLDIVASDRKGTSRGCLWIENPGHDHARLGRWPVHRIGLGDREY
                     MFLTVADLDADGLRDVLAATAGGELVYYRRAAAGPPEWESYTIRLPADAGTGKGVGIA
                     DVGRRSDRHRFQL"
     gene            complement(1070..1753)
                     /locus_tag="P5316_19345"
     CDS             complement(1070..1753)
                     /locus_tag="P5316_19345"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRR87170.1"
                     /translation="MHDWMLEDYRASRRQMIDEQIRGRGIEDPRVLAAMEEVPRELFV
                     PPDKRDAAFSDAALPIACGQTISQPYIVAYMTVCLEVEPHHRVLEVGTGSGYQTAVLA
                     RLAGEVYTIERVGPLLLAAKSLLDSLGVRNVHYLRGDGSLGWPEFAPFDRIMVTAAAP
                     RVPEALVRQLSEGGRLVIPVGGHSQQTLTQVRIENGRVIETPGLPVRFVKLVGDQAWS
                     TNTPPSEEE"
     gene            complement(2124..3146)
                     /locus_tag="P5316_19350"
     CDS             complement(2124..3146)
                     /locus_tag="P5316_19350"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR87171.1"
                     /translation="MSDAINIAVKPAGRSRQLTTFNLEKRSKTVVLPPGRPVTVGEVQ
                     GAGYIGRLWLTFPGWFWQHWHTTAAIDPTMLKSLILRIYWDGAATPAVECPMGDFFGA
                     GQCEFNSFTSRYVGTSSGGFYSHWPMPYAKGFRIEVENRHPRNGTDIFVNANYQELDA
                     MPERTGYFCTQFRTGRRKPGEEALIVDTTGSGHFVGMMLHMQGELLHYLSFLEAPEYV
                     YIDDDWDKPRFTGTGLEDYFNGGWYFRDGEFNGPLHGVPLKDPLRSMVTMYRFHEADA
                     IAFDRRFKLTFVNPWAVDRLKPYWYSSVAYYYCNAPAPVQPPMPPHEEVMKLYRTRDT
                     DHISIP"
     gene            3483..4589
                     /locus_tag="P5316_19355"
     CDS             3483..4589
                     /locus_tag="P5316_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR87172.1"
                     /translation="MNRILDRLGSSLCLTIVPVTLPAIMPAIAGGAAASQPASRWTAE
                     KANQWYAALPWLVGCDFIPSTAVNQLEMWQAETFDEATIDRELGWAADIGMNTVRVYL
                     HDLAWQADPDGFKQRMNRFLRIADKHHIRPLFVIFDDCWNDNPKIGKQPDPIPGVHNS
                     GWMQSPGKAVVNNPAEWGRLERYVRDVIGTFGQDRRVLLWDLYNEPGNSEQHLKSLPL
                     LKKVFEWARAAGPSQPLSAGLWYDNKELNEFQLAASDVITFHNYNDAKSLTDQIAELK
                     KHGRPVICTEWMRRPVSVVQSHLPIFARHRVGCINWGLVSGKTQTIYPWGSKQGAPEP
                     KPWFHDLFHKDGTPYDPAEIAMFKELSAKIRKGQ"
BASE COUNT         1208 a         2218 c         1929 g         1137 t
ORIGIN      
        1 gcgtttgcct cttgattgct cacaactgaa aacgatgtcg gtccgaccgt cgcccgacgt
       61 ccgcgatgcc cacacccttg cccgttccgg catcggccgg caacctgatt gtgtagcttt
      121 cccactccgg cggcccggcc gctgctcgcc gatagtacac cagttcaccg ccggccgtcg
      181 ccgcgaggac gtcccgaaga ccgtcggcat ccaggtccgc cacagtcagg aacatgtact
      241 cacgatcgcc cagcccgatc cgatgtaccg gccaccgacc gagacgggcg tgatcatgcc
      301 ctgggttctc gatccacagg caaccgcgag aagttccctt gcggtccgat gccacgatgt
      361 ccaggtcgcc gtcgccatcc atgtcttcgg cgatcagcga catgatccag cccgcatcgt
      421 acaaggggtg ccacttccag gcgttcagat cgcgcgcttc gttcggggcc tcgaaccagc
      481 cgattccggc gccatcgttc ttgccgccga ccacaaggtc gatgccattg cacccatcga
      541 cctgcatcgg caggcagaac atccattggc agacgccctt ggacgccggc aagccctctg
      601 tcttccatgc cgttgggtcg aggtatcgcg accgctcgcg gggtgcccaa tgaaggaaga
      661 cggttttctc cttgccctcg caacaactga ccacgtcgaa ggccccgtcg ccgtcgaggt
      721 cggcgaaaac ggcgtcctcc ggcgaactca cctgtcccac cgtgaccgcc ggccacggtc
      781 gtctggccgc ccgcgtgccg ggatgcaggt agactcggat caggccgcct tcctcccatg
      841 cggcagcgac atcgggaagc cggtcaccat tgacgtccgc cagtcgaacg ccgtcggcac
      901 cggcagatga gttgtcgatc acgtgcaggg gccacgccga tagagcgtcg cggcccgtcg
      961 atgcacagcc gaaggcaatc gaaagcatcg cagggaagag gaaccgacgg acgccgccga
     1021 gtgcttgcga gctcggccgg tacgaggctt gtgcttgctt catcgcgtct cattcctcct
     1081 cgctcggagg tgtgttcgtc gaccatgcct gatcgccgac cagtttcaca aaccggaccg
     1141 gcagaccggg cgtctcgatc acgcgtccgt tctcgatcct gacctgcgtc agcgtttgtt
     1201 gcgaatgccc gccgaccggt atgaccagtc gcccgccctc gctcagttgc cgcaccaacg
     1261 cctctggcac gcgtggggcc gccgccgtca ccatgatccg gtcaaagggc gcgaactccg
     1321 gccagccgag gctgccgtcg ccccggagat agtggacgtt gcgcacccca agcgagtcca
     1381 gcagcgactt ggccgccaag aggagaggcc cgacgcgttc gatggtgtag acctcgccgg
     1441 ccagcctcgc cagcaccgcc gtctggtagc ccgagcccgt gccgacttcg agcacgcgat
     1501 ggtgaggctc cacctccaga cagaccgtca tgtatgcgac gatgtacggt tgcgagatgg
     1561 tctgaccgca ggcgatgggc aatgccgcgt cggagaacgc cgcatctcgt ttgtccggcg
     1621 ggacgaacag ctctcgcggc acctcctcca tggcggcgag aacgcgggga tcttcgatgc
     1681 cccgcccacg tatctgctca tcgatcatct gtcggcggga agcacggtaa tcttcaagca
     1741 tccaatcgtg catgacggca tggcctgacg gacagtaatg aagtgctgcg cattgtacga
     1801 gcgtcgacgt acgcgcgtcc atccgggggc acgacatggg ctgcggaggg ggtgaaccgg
     1861 gtgttgagga gcgtttcgcc gttttcgcag agccggtcgg gcaccggccg acaggccgcc
     1921 cgcgacaccc gcgacgacaa agggtcggcg ggcacccgcc gcaatgggcc cgcagcagaa
     1981 tcggcgggca aacgtgaggc ttccctttca gaaccgcgcc cctcgcggag cggcctcctc
     2041 gggcgtgcgt ccgcctcgcg gtcttgcgaa tggctcacgc tccggcttcc gagacaggca
     2101 gccgcgccgt ctgctaccga cgctcacgga atcgagatgt gatccgtatc cctggtgcgg
     2161 tagagcttca tcacctcttc gtgcggcggc atcggcggct gtaccggcgc cggcgcgttg
     2221 cagtagtagt aggcgaccga tgagtaccag tacggcttga gccggtcgac cgcccacgga
     2281 ttgacgaacg tcagcttgaa ccgcctgtcg aacgcgatgg catccgcctc gtggaagcgg
     2341 tacatggtga ccatcgaccg cagagggtcc ttgaggggca cgccgtgcaa aggcccgttg
     2401 aactcaccgt cccggaaata ccagcccccg ttgaagtagt cctcaagacc cgttccggta
     2461 aatcgcggct tgtcccagtc gtcgtcgatg tagacgtact cgggggcttc cagaaacgac
     2521 aggtaatgca gcagctcgcc ctgcatgtgc agcatcatgc cgacgaagtg accgcttccg
     2581 gtggtgtcga cgatcagggc ttcttcgccc ggcttgcggc ggccggtccg gaactgggtg
     2641 cagaagtagc ccgttctttc tggcatcgca tcgagttcct gatagttggc gttgacgaaa
     2701 atgtccgtgc cgttgcgcgg gtggcgattc tcgacctcaa tgcggaaacc cttggcatag
     2761 ggcatcggcc agtggctgta gaacccgccg ctggacgtgc cgacgtaccg ggaggtgaac
     2821 gagttgaact cgcactggcc ggcgccgaaa aagtcaccca tcggacactc gaccgccgga
     2881 gtggccgcgc cgtcccaata gatccgcaga atcaacgact tgagcatcgt cggatcgatc
     2941 gccgccgtcg tatgccagtg ttgccagaac cagcccggaa acgtcagcca gaggcggccg
     3001 atgtaaccgg caccttgaac ctcgccgacg gtcaccggcc gcccgggcgg caggacaacc
     3061 gtcttcgacc gcttttccag attgaaggtc gtcaactgcc gggatcttcc ggccggcttg
     3121 accgcgatgt tgatggcatc cgacatttgt agacttcctc acgtcaacag accatttgtc
     3181 acgagtccgc aatttgcggt agactaccac acgcgggccc aacatgcaca gcccgcgaaa
     3241 cgagaatcgt gacaacaggt gcatccgcca gacagtggcc gtccactggg gccccgcggt
     3301 gagcaatgga aacagcctgg cgattcctcg gcggaaccca gccatgcccg ccatggacaa
     3361 gccccgttgc ggccaacgag aatagcccgg cgattcgtcg ctgggattct ggccgagcac
     3421 cggcgcgcgg atgcgagtcg cggttcagcc cgaggcatcc ctcatcgaac ggaaaggcaa
     3481 tcatgaacag aattcttgat cggcttggtt cgagtctgtg cctgacgatt gtgcccgtca
     3541 ctcttccggc gatcatgccg gccatagccg gcggcgctgc cgcctctcag cctgcctccc
     3601 gatggaccgc cgagaaggcc aaccagtggt atgccgccct gccgtggctc gtcgggtgcg
     3661 acttcatccc cagcacggcc gtcaaccagc tcgaaatgtg gcaggcagag acattcgatg
     3721 aggccacgat cgatcgcgaa cttgggtggg ccgccgacat cggcatgaac accgtccgcg
     3781 tgtaccttca cgatctcgcc tggcaggccg atccggacgg cttcaagcag cggatgaacc
     3841 gcttccttag aatcgccgac aagcaccaca tcaggccgct gttcgtcatc ttcgacgact
     3901 gctggaacga caacccgaag atcggcaagc agcctgaccc catccccggc gttcacaact
     3961 ccggctggat gcagagcccc ggcaaggccg tggtcaacaa cccggccgag tggggacgac
     4021 tcgaacgata tgtccgcgat gtgatcggca cgttcggcca ggaccgacga gtcctcctgt
     4081 gggacctgta caacgagccg ggtaacagcg aacaacacct caaatcgctg ccgctgctca
     4141 agaaggtgtt cgagtgggcc cgggccgccg ggccgtcgca gccgctcagt gccggcctct
     4201 ggtacgacaa caaggaactc aacgagtttc agcttgccgc ttccgacgtc atcaccttcc
     4261 acaactacaa cgacgccaag agcctgaccg accagatcgc ggaactgaag aaacacggcc
     4321 gcccggtgat ctgcaccgag tggatgcgcc gtccggtcag cgtcgtgcag tcgcatctgc
     4381 ccatcttcgc caggcaccgt gtcggctgca tcaactgggg tctggtctcc ggcaaaaccc
     4441 agaccatcta cccctggggc tccaaacagg gcgcccctga gcccaagccc tggttccatg
     4501 acctcttcca taaagacggg acaccctatg acccggccga gatcgcgatg ttcaaggagt
     4561 tgagcgccaa gatcagaaag gggcaatgac attcgcgaca tcatgccgat tgcgcgaatt
     4621 caaccggccg ccgacccgtg agtgtcctcg gatcagtctc cgctcgatac actccgccat
     4681 ttccggagca cccaacatgt tatccccctg accgaccatg ggtgacagtc cgttcggccc
     4741 ggtggcggtt ctcgagccga caccgcgccg ccgacgacgt ctcccgctgc tcattgacaa
     4801 cttcatagtg cccccccggc ggcggcccgg aatgaatgtc ctgtcgatcc cacggcaccc
     4861 gtccgctcga atccttcgcc tcgctttttg cgaaacaaac ccaacgcctg cggtccggac
     4921 aaaccgggat gaaggacaac ggctcacgca agtctttcgc tgctcgtatc ttacgccgcc
     4981 agatgcgcgc atgttggccc ggaatccgct cagcccggaa tccgctcggc ccggaatccg
     5041 ctcggcccgg aatccgctcg gcccggaatc ctttccgggc cacctccccc ccgctcagcc
     5101 cggaatcctc tccgggtcgc atcccctgcg gaatcctttc ccgctcggcc cggaatcctt
     5161 tccgggccac ctcccacccc gctcggcccg gaatcctttc cgggccacct cccccgcgga
     5221 atcctttccg cattcggcgt ccatggcagc acgctaaggc gtgccgggtg ccatgcccac
     5281 ggctctgcgt gggcatgcgc ctccaaatga atcctgaagc acgtagggca ggtcagagcc
     5341 ctgcggagtc gccccgacga agcagggcgc cgacctgccg ccgccctgac cgctcccccg
     5401 cgccacacaa acccaacatc aagcgcaaac cgctcggccc ggaatccgct cagcccggaa
     5461 tcctttccgg gccgcctccc ctgcggaatc ctttccgcat tcatggaaaa ccggagagac
     5521 atcgtacctg tttcgatgct tgttctggtt tccggcctgg ctgtcggagt gtaaggatgc
     5581 ggttgagggt ggtctcgagt ctggtgagaa aggcttggtt acccagcggg cggccggttc
     5641 gttcgcggtg ttgcaggcgt gatggacgat catcgggagc gggttgccgg aggaatcttc
     5701 gccagtcttt catcatgccc ggaagcggcg cgaccttggc cggccggtca tcggcgggca
     5761 gaaaaacctc tgccgtcgac ggttcctccc gctgggtaat ccgatgcgga taacccggta
     5821 caaccactcg cgcaatccga cgcatgacgc cgtcatgaaa cgacgttgac tcggccgcaa
     5881 gagacaatat ggcgtccccc ggattccccc tcggagccct ggtccgatgg cgacccgctg
     5941 caagccaaac cttgtccttc cgcaagccgg gccgactgcc caacgcggcc caatgacctc
     6001 gcgaaggatt aaggaaacgc tccgccggca gggcgccggg acaccgcgcg aacaacttgc
     6061 ccctgcaagg atcgaaccgg acacggctga aggcttccag ccgctggcgc aactgtgagc
     6121 tcaggccgct tctgctggcc aataccatac aaaacctcca gattcatgcc gacgaggcgc
     6181 caacccgagg cgcttgccgt ccctgaatcc tgcgagaccc tgcaatacgt gttttagatg
     6241 ccttatacag tgtttatgtt ttacgccggt ggcgcccagg ctgcgcttgc gcaaaaaagc
     6301 gggccgtccc caatggacgg ccctgcttgt caggtgaagt ctcgcgccgc gcgtcggcta
     6361 tcggcgcctt cgccagagca tcgccaggct gctgaggccc agcaggccaa gcgtagccgg
     6421 ctcgggagtc tgccagacct tgtcggcgcc ggtctgaaca tgctgtaaat ccgccggttt
     6481 gatgatgcgg tg
//
LOCUS       DAOTPW010000294         6457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_10325_length_6457_cov_5.360200, whole genome shotgun sequence.
ACCESSION   DAOTPW010000294 DAOTPW010000000
VERSION     DAOTPW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10325_length_6457_cov_5.360200"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1364
                     /locus_tag="P5316_19360"
     CDS             174..1364
                     /locus_tag="P5316_19360"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRR87173.1"
                     /translation="MSQSLMEPSPAVIPHRDPAEIQAYNRVNRRIHLADLAISLVYWI
                     IWCLIAADVVQWLGLPGGSRWLGLLVAAVVMLGGSVLVGLPLNYYRGFVVEHRFKLSN
                     QTPAAWLLFQVKAWLVGSIIGGIVLGGLYAAMWYTGPWWGVWVWLGVMLLSIGLAKVF
                     PLVILPLFYPSKPLERPSLAERLNELAGGAGMTITGIFNLQLSKDTKKANAMLAGMGS
                     SRRVYLADTLLDSFDDDQIAVVFAHELGHHTRGHIWKLIGMSAVASSLMVALIWWRLN
                     PFAGSSDTADWSAAIAAFPQVMLISTLFPLIIGPITNAVSRRFERQCDGDALRLTDNP
                     QAYRTAFEQLGRLNLDDPTPPWWEVVFFDDHPPLGVRIKMADEYERAAARGQGMCNEG
                     SLSA"
     gene            complement(1534..1839)
                     /locus_tag="P5316_19365"
     CDS             complement(1534..1839)
                     /locus_tag="P5316_19365"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR87174.1"
                     /translation="MGTWAIGGRDWGKADDEVSGRAINKALDCGMTLPDTADVYGFRR
                     SEELTGRVLEDRGRPDVITATKAGNDFYDATSADDKGYGPIMQNYDREYLSRRPRKV"
     gene            complement(1930..2979)
                     /locus_tag="P5316_19370"
     CDS             complement(1930..2979)
                     /locus_tag="P5316_19370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR87175.1"
                     /translation="MSITRRELIGGLSAAGAAASLPEFLKDPFPAVQPSGQVEPFTFV
                     HLTDMHVTRKRKGDEGYRKCIETLRQLKPRPAFILMGGDMAFDGNYTAKSEFEECVRL
                     YKGITDASGIPYYNCMGNHDALGWSSRRKVPVDDPDIGKKMIMDLLGWKKSYYSFDHG
                     GWHFVVLDSIFPVGGKDNPTYEPRIGPEQLEWLAYDLGKAGGRPTVAVTHIAASCNRG
                     QLLGDPNYKAMDGSMVLWDTRELRIILERHKVKALLQGHSHNIEEMRYHGVWYLTSAA
                     ASGAWWAGDWVGSPPGYTVFRCDGDKLSWEHVEFSWEPRLEEADTLERKKLDEFQAFR
                     REQQRLLEKERAGIR"
     gene            complement(3120..3971)
                     /locus_tag="P5316_19375"
     CDS             complement(3120..3971)
                     /locus_tag="P5316_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRR87176.1"
                     /translation="MRWILLNTVFSALAGCASLDMDKAHRPTDAGITRYARFCAGGQE
                     AYGIVEGDTIHEIKGDLFGARQRTGRSYRLAEVKMLVPARPTKVLGMAGNYTSHLSTQ
                     PKPSNPELFFKAPSCLIGPGDAVVLPAGSKEVHLEGELVVVMGKRARNVSEAEALDYV
                     LGVTCGNDISARDWQANDRQWWRAKGSDTFGPCGPFVVAGVNYDDLLLTSRVNGQVTQ
                     QQRTSDMIFNCSQIVSFASRHVTLEPGDLIFTGTMGKTPAIKPGDVLEVEIEHVGVLR
                     NPVVAEK"
     gene            4182..5420
                     /locus_tag="P5316_19380"
     CDS             4182..5420
                     /locus_tag="P5316_19380"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRR87177.1"
                     /translation="MVDANNRTRGRIGPLSRRTILGAGLACGVEALPQTPATQSTQPE
                     ALPRRPLGRTGAQITRLVMGGSFSEYGPRLLDFAFRSGIRSFDMGDFYAGYKAETILG
                     EWVRKRANRKDVFIINKARTTEPDAFVKRLNEALSKMKIDTIDAFLLHGIQDPRLPLD
                     RDGVWSKTKEKLIRDNKIRFMGFSVHAEMPSRVACLNNAARSKWVDVVMVACDPGLIR
                     SSPDLNSALDACAKAGIGLLAMKTGRGLGAPATQPASVREAFRKLGHSPHTAMLAGIW
                     SDERFAAACSEMPSIQIIEENAAAARSLHKPFDAEQWKTFDEAAHGLSRATCPGCDGS
                     CRKAAGTQADLCAITRYLAYYRESGSRDLARELYSALSPELRDWAGADLRAASRACRA
                     HVDFESLIPLAKRLLESEPA"
     gene            complement(5552..>6457)
                     /locus_tag="P5316_19385"
     CDS             complement(5552..>6457)
                     /locus_tag="P5316_19385"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR87178.1"
                     /translation="GHWPRQLYIREARRMVGEYVMKQADLQTDRTKPDSIGMGSYNSD
                     SHHVQRIPTPDGAVINEGDMQVPVQPYEISYRALLPKQGECDNLLVPVCFSASHVAYS
                     SMRMEPQYMIMGHAAGLAAAWAVRNDTSVGKVDIAWLQKRLREQGQVLSMGEAVGEGL
                     NPRSMGGIVVDNQAAKVIGDWRSGMSVRPFVGSEYLHDNNSGKGTAAVRFVPTLPASG
                     LYEVRIAYAANPNRASNVPVVIHSAEGDKTVTLNQREAPTEPPFVLLGRFRFDAGSEG
                     YVEIRNEGTNGHVIADAVQWVPAVP"
BASE COUNT         1119 a         2153 c         1884 g         1301 t
ORIGIN      
        1 gcctgccctg cccggctgag gccgtgtgcc ggcatagggg cgcgtcggac ttgccaaggc
       61 ccggacaacc gcggatgccg tgagcccgta ccaggcgtca ccggccggca gcacagcggg
      121 accttctcaa ttcggcggcc ggcgattatc ctttggacat gagtgaccag cccttgtctc
      181 aatcgttgat ggaaccttcg ccggccgtga tcccgcaccg cgatcccgcc gagatccagg
      241 cgtacaaccg cgttaatcgt cgcatccatc tggcggatct ggcgatttcc ctcgtatatt
      301 ggattatctg gtgcctgatc gccgctgatg tggtccaatg gctgggtctg ccgggcggat
      361 ctcgttggct ggggcttctg gtagcggcgg tcgtgatgct gggcgggagc gtgctggttg
      421 gcctgccgct gaattattac cgcggctttg tcgtcgagca tcgcttcaaa ctcagtaatc
      481 agacgccggc ggcttggctg ctattccagg tgaaagcgtg gttggtaggc tcaatcatcg
      541 gcggcatcgt gctcgggggt ctttacgccg caatgtggta caccggccca tggtggggcg
      601 tgtgggtgtg gctcggtgtc atgctgctca gcatcggact ggccaaggtg ttccccctgg
      661 tgatcctgcc gctgttctat ccctccaagc cgctcgaacg gccaagcctg gccgagcggc
      721 tcaacgaact ggctggcgga gccggaatga ccatcaccgg tatcttcaac ctgcagctca
      781 gcaaggatac caagaaggct aacgcgatgc tggccggcat gggctcttcg cgccgcgtgt
      841 accttgccga cacccttctc gactcctttg atgacgacca gattgccgtg gtcttcgctc
      901 acgaactggg ccatcacacc cgcgggcaca tctggaagct catcggcatg tccgctgtgg
      961 ccagttcact gatggtcgcg ctgatctggt ggcggctcaa cccgttcgcc ggcagcagcg
     1021 acaccgcaga ctggtccgcc gccatcgccg ccttcccgca ggtcatgttg atctcaacgc
     1081 tcttcccgct gatcatcggg ccgatcacca acgccgtcag ccgccgtttc gagcggcaat
     1141 gcgacggcga tgccctccgc ctgaccgaca acccccaggc ctaccgcacg gccttcgaac
     1201 agcttggccg tctcaacctc gacgatccca ccccgccctg gtgggaggtc gtgttcttcg
     1261 acgaccatcc gcctctcggg gtgcggatca agatggcgga cgagtacgaa agagcagcag
     1321 cgagggggca aggcatgtgc aacgagggat cattgtcggc gtagggtctg ctgccttggc
     1381 gggtgccggc ttgccgtgcc gtggcaaccc gcgtggcgga ccttgccctg gttcttgagc
     1441 gttgaaagcg cgtcccacgg atcgtcgcgt tccggccgtg cgaatccgga ctgtgcagtt
     1501 gcaggatgtc gacaatctcc acccgcagcc gcctcagact tttctcggac gccgagatag
     1561 gtactccctg tcgtagttct gcatgatcgg tccatacccc ttgtcgtcgg cgctggtggc
     1621 atcgtagaaa tcgttgccgg ctttcgtggc ggtgattaca tcgggtcgcc cacggtcctc
     1681 cagcaccctg ccggtgagtt cctccgaacg cctgaatccg tacacgtcgg ctgtgtccgg
     1741 cagcgtcatc ccgcagtcga gggctttgtt gatggctcgt cccgaaacct cgtcgtcggc
     1801 tttcccccaa tcacgaccgc cgatcgccca cgtacccaaa ccgaccgtcg agacgaccgg
     1861 tccgcgtttg ccgaaccgcc cgtaaagcag tgatgtacct cggatccgcc ggatgcctgc
     1921 cgattgcggc tacctgatcc cggcccgctc tttttccagc agccgttgct gttctcgccg
     1981 gaaagcctga aactcgtcga gcttcttgcg ttcgagggta tcggcctctt cgagccgcgg
     2041 ttcccacgaa aactcgacgt gctcccagct cagcttgtcg ccgtcgcagc ggaacaccgt
     2101 gtagccgggc ggcgaaccaa cccagtcgcc cgcccaccac gcgccgctcg cagccgcgct
     2161 cgtcaggtac caaaccccgt gataccgcat ctcctcgatg ttatggctgt gtccttgcag
     2221 cagcgccttg accttgtgcc gttccagaat gatccgaagc tcccttgtgt cccagagtac
     2281 catgctgccg tccatggcct tgtagttggg gtcgcccaac agttggccgc gattgcacga
     2341 tgccgcgatg tgcgtgacgg ctacggtcgg tcgaccgccg gccttgccaa gatcgtacgc
     2401 cagccattcc aactgctccg gcccgatccg cggctcatac gtcgggttgt ccttgccgcc
     2461 gaccgggaaa atgctgtcga gcaccacaaa gtgccagccg ccatggtcga agctgtagta
     2521 gctctttttc caacccagca ggtccatgat catcttcttg ccgatgtccg ggtcgtcaac
     2581 cggtaccttg cgtcgcgacg accatcccag cgcgtcgtgg ttgcccatgc agttgtagta
     2641 cggaatgccc gaagcgtccg tgattccctt gtacagccga acacactcct cgaattcgga
     2701 cttggccgtg tagttgccgt cgaaagccat atcgccgccc atcaggatga acgccggcct
     2761 gggcttgagc tgccgtaggg tctcgatgca cttacggtat ccctcgtcac cttttcgctt
     2821 gcgagtcacg tgcatgtcgg tcaggtgaac gaaggtaaac ggctcaactt gcccgctggg
     2881 ctgcacagcc gggaatgggt ccttgaggaa ctcaggtaac gacgccgccg caccggccgc
     2941 agacaggccg cctatcagtt cgcgtcgcgt gatgctcatt cattcctcct cacgggcgtg
     3001 ccgggttcac acaggccttg tcgcggaaac ccagatgatg agaccaggtt ttcagtcgcc
     3061 agtgtcactt ttcagtttcg cttttgaccg cctactgatc actgacaact gataactgac
     3121 tacttctccg ccacaacggg attcctcagc accccaacgt gctcgatctc gacctccaac
     3181 acatcgcccg gcttgatggc cggggtcttt cccatcgtcc ccgtaaaaat cagatcgccc
     3241 ggctccagcg tcacgtgacg gctggcaaag ctcacaatct gcgagcagtt gaagatcatg
     3301 tcgctggtcc gctgctgttg cgtcacctga ccgttgaccc gagaggtcaa caacaggtca
     3361 tcgtaattga cccccgcgac cacgaacgga ccgcacggcc cgaacgtgtc gctgcccttg
     3421 gcccgccacc actgccggtc gttggcctgc caatcgcggg cgctgatgtc attgccgcaa
     3481 gtaaccccca gcacgtaatc gagggcctcg gcctctgaca cattcctggc ccgcttgccc
     3541 atgacaacga ccagttcgcc ctccagatgg acttccttgc tgccggcggg caggacaacg
     3601 gcgtctcccg gcccgatcag gcaggagggc gccttgaaga acaactcggg gttgctcggc
     3661 ttgggctgcg tgctcaagtg gctggtgtag ttccctgcca tccccaacac tttcgtcggg
     3721 cgggctggaa ccagcatctt gacctcggcc aggcggtatg atcgtcctgt tctctgccgg
     3781 gctccgaaaa ggtccccttt gatttcgtgg atcgtgtcgc cctcgacgat cccgtaagcc
     3841 tcctgaccgc ccgcgcaaaa acgggcatac cgcgtgatcc cggcatcagt cgggcggtgc
     3901 gccttgtcca tatcgagcga ggcgcacccg gctaaggcgg aaaaaaccgt gttgagcaga
     3961 atccatctca tcgttgagct cctcaatcgt atatgggggc aattcatgtt tgttcccggc
     4021 ggccggctcg acgattgaca gtgaaccgaa acccgcgtcg ccccgcgccc atttgtaatg
     4081 catacgggct tctctcgcca ctggcgaacc ggccggcttt tcacggcccg ggcatcgcgg
     4141 tactctggaa ggcgccacga catcagatag cggaggatgc gatggtggac gcgaacaaca
     4201 ggacgcgcgg gcgaatcgga cccctctccc gtagaaccat acttggtgcg ggcttggcgt
     4261 gtggcgtcga agcattgccc cagacgcccg ccacccaatc gactcagccg gaagcccttc
     4321 cccgccgccc cctcggcagg accggcgccc agatcacccg gctggtcatg ggcggcagct
     4381 tctcggagta cggcccacgc ctgctggact tcgctttcag gtcgggcatt cgctccttcg
     4441 acatgggcga tttctacgcc ggctacaagg ctgagaccat cctcggtgaa tgggttcgca
     4501 aacgagccaa tcgcaaggat gtcttcatca tcaacaaggc cagaactacg gagcccgacg
     4561 ctttcgtcaa gcgcctgaat gaggctcttt ccaagatgaa gatcgacacg atcgacgcct
     4621 tcttgctgca tggtatccag gatccacgac tgcccctcga tagggatggc gtttggagca
     4681 aaaccaagga aaagctcatc cgcgataaca agatccgctt catgggcttc agcgttcacg
     4741 cggagatgcc gtcgcgagtc gcctgtctga acaacgctgc caggagcaag tgggtggatg
     4801 tggtgatggt ggcatgcgat ccggggttga ttcgctcctc gccggatctg aacagcgctc
     4861 tggacgcatg cgcaaaggcg ggtatcggcc tgctggcaat gaaaaccggc cgtgggctgg
     4921 gtgccccggc tactcagccc gcgagcgtaa gagaggcctt ccgcaaactg ggccattcgc
     4981 ctcacacggc catgctggcc ggcatctgga gcgacgagcg gtttgccgcg gcctgctcgg
     5041 agatgcccag tatccagatc atcgaggaaa acgccgctgc cgcgaggtct ctccataagc
     5101 cgtttgacgc cgaacaatgg aagaccttcg acgaggccgc gcacggtctt tcgcgagcga
     5161 cgtgtcccgg ctgcgacggc tcatgccgca aggccgccgg cacgcaggcc gatctgtgcg
     5221 ccatcacccg ctacctggcc tactatcgcg agagcggtag ccgcgacttg gcccgcgaac
     5281 tctatagcgc cttgtctccc gaactccgcg attgggccgg cgccgacctt cgcgccgcct
     5341 ctcgggcttg ccgcgcacac gtggatttcg agtcgctcat cccgctggcc aaacgcctcc
     5401 tggaatcaga accggcgtga gcagcaacgc caccgcatgg ttgggcaacg tcgaggtcgc
     5461 atcagcgacc gagagctacc gcacccggat gctcgaaaca gcaggtctgt cctgtccctg
     5521 gtggccgtaa cctggccctg tgccatacct ctcacggcac cgccggtacc cattgaaccg
     5581 cgtccgcgat cacgtgtccg ttggtccctt cgttgcgtat ctcaacgtag ccttctgacc
     5641 cggcgtcgaa gcggaatctg ccgagcaaga cgaacggcgg ctcggtcggg gcctctcgct
     5701 gattcagcgt cacggtttta tctccctcgg ccgagtgaat cactaccggc acgttgctcg
     5761 cccggttcgg gttggcggcg taagcgatgc gcacctcata cagcccgctc gccggaagcg
     5821 tcggtacgaa tcgcacggcc gccgtgccct tgccgctgtt gttgtcgtgc agatactcgc
     5881 tgcccacgaa cggcctgacc gacattcccg accgccagtc gcctatcacc ttcgcggcct
     5941 gattgtcaac gacgatgccg cccattgatc ggggattcaa gccttccccg actgcctcac
     6001 ccatcgacag cacctggccc tgctcccgga gccgtttctg taaccatgcg atgtccacct
     6061 tgcccaccga cgtatcgttt cggacggccc acgccgccgc caatccggcc gcgtgcccca
     6121 tgatcatgta ctgcggttcc atccgcatcg acgagtaggc cacgtgcgac gccgagaagc
     6181 acaccggcac cagcaggttg tcgcactccc cctgtttggg cagcagcgcc cgatacgaga
     6241 tttcatacgg ttggaccggc acttgcatgt ccccctcgtt gatcaccgcc ccgtccggag
     6301 tgggtatgcg ctggacgtga tgcgagtcgg aattgtacga acccatgccg atcgagtccg
     6361 gcttggtgcg gtctgtctgc aggtcggctt gcttcataac gtattcgccg accatgcgcc
     6421 gggcctcgcg aatgtagagc tgtctcggcc agtggcc
//
LOCUS       DAOTPW010000295         6377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_10488_length_6377_cov_4.834704, whole genome shotgun sequence.
ACCESSION   DAOTPW010000295 DAOTPW010000000
VERSION     DAOTPW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10488_length_6377_cov_4.834704"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="P5316_19390"
     CDS             <1..789
                     /locus_tag="P5316_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87179.1"
                     /translation="SPHHQTGGFTACSDRRRQEAQRGFRKALMAHQTRPNAGTQGRFA
                     AIHYWLGKSYVRLGDMGNARVAMRKAQSCEAGPESNALFNLQRLQEDNLIIVIQIGAG
                     AGLIPQGGDWQQTAYKPTPYAERAAEVFIDGQHVGSAVMIADLWEQVSTQGQTEEQRI
                     QASKAAAKSIVQSLPYISLLGVAWDVTGDMRSWALLPDRILVWSGKVPSGDHTVILRF
                     YDDKDFELTRYRQTWYFVPVPAEGDCVVVTRAARDKCNICRKEQ"
     gene            791..1282
                     /locus_tag="P5316_19395"
     CDS             791..1282
                     /locus_tag="P5316_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87180.1"
                     /translation="MRFILCILFGALFAAQVGCTSHSWTKIERTYEVGYHDVFQRMPE
                     ECKKWMMSVKKSQPEAGLIVLESHRVHDQILTGSVINMIAGDEVILKVQQVTPTTTKI
                     FIDSKAKGQIGPDFGRTDRNVTTVADALDKVWAQVDATTEERGEKRPGSSASQPNAQA
                     KTQ"
     gene            complement(1593..2024)
                     /locus_tag="P5316_19400"
     CDS             complement(1593..2024)
                     /locus_tag="P5316_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87181.1"
                     /translation="MSTVFDYDWFTGTAFEQQKAVARELCARKKFPGVTQDHPRYQDY
                     HKFLSELETRVLIYLRDGFSYEMKQLVDLGLRAMLTFECEPVDEQYKVGAFVVSALFE
                     EIVRVEVFAVHPSEKPEDTPMITGFRSRPSEPLPRDDGREP"
     gene            2311..3471
                     /gene="queG"
                     /locus_tag="P5316_19405"
     CDS             2311..3471
                     /gene="queG"
                     /locus_tag="P5316_19405"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRR87182.1"
                     /translation="MRRMTPAEKTVLVRELALEAGFVRVGVVRAVPLARAGYFREWLA
                     RGCAGSMNYLARWQDLRADPRLLLEGARSIIVVAWRDGGLAGEARAFERACERGRAWS
                     EPPEAVETSGSTFNHSGNHSAGLGRRGDLDAALRHGVDGPQTSSEPASDEPRGRVARY
                     AWGRDYHRAVRNRLRRLGDRLREVMGEPLRARVCVDTAPIIEREWAAAAGIGWIGRNT
                     MVIDSRIGSMFMLGEIITTLELEPTLPVKDLCGNCMRCVEACPTGALTRPYQMDASRC
                     ISYLTIEHRGEISEELSRRMGNWVFGCDVCQEVCPWNRHAAGQAAESVNVRNPLVPSP
                     LLSDLCKLDADQYDRFLRGSAMKRATLEMLRRNARIALGNCRGNKKGDGTAE"
     gene            complement(3582..4025)
                     /locus_tag="P5316_19410"
     CDS             complement(3582..4025)
                     /locus_tag="P5316_19410"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR87183.1"
                     /translation="MLPVRVFQNCVNDMCSAPLIRWSRLPRLFDDLFDETALHAAGAL
                     PVDVRQEGDKWILEAEVPGIAREDLNITVENGVLTIGGKYAQEAAKENAEYYVRERRT
                     GEFSRSFRLPETADSENVTAQLANGVLTLTIPTREDAKPRRIELK"
     gene            complement(4439..6106)
                     /locus_tag="P5316_19415"
     CDS             complement(4439..6106)
                     /locus_tag="P5316_19415"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRR87184.1"
                     /translation="MSKIVTLPELLHRVAEARAAGKTVVHCHGCFDIVHPGHVRYLDF
                     ARRQGDVLVVSLTGDSQVSKDLQRPYIPQELRAENLAALEAVDLVYVNDHPTATDLLR
                     QLKPDVYVKGREYEQSSDPGFLAERQVVSAYGGRVMFSSGDVVFSSTRLIEALSRDPA
                     LDIDRLTTICRRHEIDRNSTEALLNSFASLRVLIVGDVLLDHYVLCDATDLAGEAPMM
                     SLTQLEERVYVGGTGIIAQHVAGLGAKPYLLSACAADEATRQIRELLARIGAETHWVP
                     TQPHLPRKTRYLVDTTKVMRVEHGRYHPLDSLAEEKAAQWVKSIIDRIDAIIISDFGY
                     GTITGGLIGRIREIAKPKAPVVTADAGSPRGRLLDFKDADLLCPAERTLRTSMHDFER
                     GLSFVAWDILQETNARELLVTLGRKGLVVFSRQSQDNHRPEWRGRLRSEYLPMLADHL
                     VDSLGCDGALHAATTLALAAGGNLMQAAYLGSAAVALEYACLGNEPINANMLRRWLSM
                     RLELSRSARRRPGEALRFPASPPDDRPPTRCDLTAARTQADPIATQT"
BASE COUNT         1148 a         1745 c         2231 g         1253 t
ORIGIN      
        1 tcaccgcatc atcaaaccgg cggatttaca gcatgttcag accggcgccg acaggaggct
       61 caacgggggt tccgtaaggc cctcatggcc caccagacgc gccccaacgc ggggacacag
      121 ggccggtttg cggcaatcca ctactggctt ggcaagagtt acgtacgcct cggcgacatg
      181 ggcaatgccc gcgtcgcgat gcgcaaagcc caatcctgcg aggctggacc ggagtcaaac
      241 gccctgttca accttcagcg gctccaggag gataatttga tcatagtcat ccagattggt
      301 gcgggagccg gcctgatacc ccaagggggt gattggcagc agacagcgta caagcccacg
      361 ccgtacgccg aacgcgcagc cgaggtcttc attgacgggc agcatgtcgg cagtgcggtc
      421 atgattgcgg atctctggga acaggtatcg acccaggggc agacggaaga gcagagaatc
      481 caggcttcca aggcagcagc caagagtatt gtacaaagcc ttccctacat cagtcttctc
      541 ggtgttgcct gggatgtgac cggcgacatg aggtcttggg ccctgctgcc tgatcgtatt
      601 ctggtctggt caggcaaggt gccaagtggc gaccataccg tgattctcag attctacgac
      661 gacaaggatt tcgagctgac cagataccgt caaacgtggt atttcgttcc agttcctgcc
      721 gagggcgatt gcgttgtcgt cacccgcgca gcgcgagata agtgcaatat atgcagaaag
      781 gagcagtgac gtgcgattca tcctgtgcat tcttttcggc gcactttttg cagcgcaggt
      841 ggggtgcacc agccactcat ggaccaagat cgagcggaca tacgaagtcg ggtaccacga
      901 cgtctttcag cgaatgccgg aggagtgcaa gaaatggatg atgtccgtca agaagagcca
      961 gcccgaggca ggcctcatcg tcctggaatc acatcgtgtc catgaccaga ttttgaccgg
     1021 ctctgtgatc aacatgatcg ccggcgacga ggtaatactg aaagtgcagc aggtgactcc
     1081 gactacgacc aagattttta tcgacagcaa agccaagggg caaatcggcc cggacttcgg
     1141 caggacggac cgtaacgtga ccaccgtcgc ggatgccttg gataaggtgt gggctcaagt
     1201 ggacgccaca actgaggagc ggggcgaaaa acgtcccggc agcagtgcat cgcaacccaa
     1261 cgcacaggcg aagacgcagt agtcactgac cgtccgaaga gctgtcatga cggtcctctt
     1321 ggcaaggcgg tcttaacctc acgagcgaga tggagacgcg ggcccgaaga tgccatggat
     1381 tgtcgttccg catggaccgc ggtccggcag gctgctccgg ggtggttggg gcggccgttc
     1441 ggtcaggcag ggggtggtag cgggggctgg agacgggcag ggggcgtcgg gcgtgattta
     1501 cgctgcgtac gcgaaaggtg cagcagaaga caggggcagg cagcgctgcc cacagactgg
     1561 acggcggacg cgcggaacca ggccgtcggc ggttacggtt cgcggccgtc gtcgcgaggt
     1621 aacggttcgc tcggccggct tcgaaacccg gtgatcatgg gcgtgtcttc gggcttttcc
     1681 gatggatgga ccgcgaagac ctcgactcgt acgatttcct caaaaagcgc cgagacgaca
     1741 aacgcaccga ccttgtactg ctcgtcgacg ggctcgcact cgaacgtgag catggcccgc
     1801 agtccgaggt cgacgagctg tttcatttcg tagctgaacc cgtcgcggag gtagatgagg
     1861 accctggttt ccaattcgct gaggaacttg tggtagtcct ggtagcgagg gtgatcttgg
     1921 gtgacgccgg gaaacttttt tcgggcgcac aactcgcggg ccacggcttt ttgttgttcg
     1981 aaggccgtgc cggtaaacca atcgtagtcg aagacggttg acatgcctgg atgctgctgc
     2041 ctctggctgt tgttgacccg ttctgccccg ggtccggggc gcgccttggt ccgggcgcga
     2101 ggcgtaaccg tagatgatcg gacagggcgt gtcaatgaag ggggtaagaa gcgggcgggg
     2161 ggccgtgaag gtcagggtgg ggcgacgggg cctgtgcggc gccgtacggg ttgagagtat
     2221 cggcttccgg tggcctggca agggttcggg gttgggagtc tgggtttggg agtttagata
     2281 agatgggttt caggcaggct cggagatacg atgaggcgga tgacgccggc ggagaagacg
     2341 gtgttggttc gagagttggc cctggaggcg ggttttgttc gcgtcggggt ggtcagggcc
     2401 gtgccgttgg cccgggccgg gtatttccgt gaatggttgg cgcggggctg cgcgggctcg
     2461 atgaattacc tggcccggtg gcaggatctg cgagcagatc cgcgcttgct gcttgagggg
     2521 gcccggtcga ttatcgtggt agcgtggcgg gatgggggct tggcaggcga ggcacgcgca
     2581 ttcgaaaggg cctgtgaaag aggtagggca tggtcggagc caccggaggc agtggagacc
     2641 tccgggtcca cgttcaacca ttcgggcaac cattcggcag gtctcggtcg ccgcggcgac
     2701 ctcgacgctg ccctacggca cggcgtcgat ggtcctcaga ccagctccga gcccgccagt
     2761 gatgaacctc gggggcgcgt ggcgcggtat gcgtgggggc gggactatca ccgggcggtt
     2821 cgcaataggt tgcgtcgcct tggcgaccgg ttgcgggaag tcatgggcga gccgctgcgt
     2881 gcgcgggtgt gcgtggatac cgccccgatc atcgagcgag aatgggctgc cgccgcgggg
     2941 atcggctgga tcgggcggaa cacgatggtg atcgattcgc ggatcgggtc gatgttcatg
     3001 ctgggcgaga tcatcaccac gctggagctg gaaccgacgc tcccggtcaa ggatctttgc
     3061 ggtaactgca tgcggtgcgt ggaggcgtgc ccgacggggg ccctgaccag gccttaccag
     3121 atggatgcct cccgctgtat ttcttatctg accatcgagc atcgaggcga gatttcggag
     3181 gagctatcgc ggcggatggg caactgggtg ttcggctgcg acgtatgcca ggaagtatgc
     3241 ccgtggaatc gacatgcagc cggtcaagcc gctgaatcag tcaacgtgcg gaacccgctt
     3301 gtgccatcgc ctcttctttc ggatctgtgc aagcttgacg ccgaccagta cgatcggttc
     3361 ttgaggggca gcgcgatgaa gcgggcaacg ttggaaatgc tcagacgcaa cgcccggatc
     3421 gccttgggca actgtcgcgg caacaaaaaa ggagacggca cggccgaatg acgtcccttc
     3481 atccgaccgc accgtcccgg tgacgaacaa cctcatgcga tgcacgcggt tggcaggcaa
     3541 cacacctgcc ggatgcccct gtccgcgtgc tcgccgtgag cctacttcag ttcaattcgg
     3601 cggggcttgg cgtcctcccg cgtcggaatc gtcagcgtaa gcacgccgtt ggccagttgc
     3661 gccgtcacgt tctcgctgtc ggcggtttcc ggcagccgga aggatctcga gaactcgccg
     3721 gtccgtcgtt cgcggacgta gtattcggcg ttctcctttg cggcttcctg ggcgtacttg
     3781 ccgccaatgg tcagtacgcc gttctccacc gtaatgttca gatcctcccg ggcgattccg
     3841 ggcacctcgg cttcgaggat ccacttgtcg ccttcctggc ggacgtccac aggcagggcg
     3901 ccggcggcgt gaagcgctgt ttcgtcgaac aagtcatcga acagcctggg gaggcgcgac
     3961 cagcggatca acggagccga gcacatgtca ttgacgcaat tctggaacac tcttaccggc
     4021 aacatcgaac acctcctggg gtcttgcccc gattgctcac ggtcgcaccg gtccacgcag
     4081 gtgcttggtg agagcgtcag ttgtcgcagc agccatatgg gcaatcgttg tgccacccat
     4141 tggaatcgcc tcaacttgtt tcacagtaac gtgttacgag ttgacgaggg ggcatgccgg
     4201 gagctgcgtt gtggtgtcag actggcaggc gatggcggag ttcttcattt gacggtggac
     4261 gcaagctttg tgggggcaag atcttgcggg cgtgccatgc cgggatggca tttgccgtaa
     4321 tagatcgcca aatccgttac ccgacatcgt cgagcgaccg gagggattga gtagcaactg
     4381 tccgcggggg gacggcgtgg tccgagaagg cctcgggcgg ctcaaggtcg ggcggttctc
     4441 aggtttgggt tgcgatggga tcggcttgcg tgcgggcggc ggtcaggtcg catcttgtgg
     4501 gggggcggtc gtccggcggc gaggcgggga atcgcagagc ctcgccgggt cgtcgtcggg
     4561 cggaccggct gagttccagg cgcatgctca gccagcgtcg cagcatgttt gcgttgatcg
     4621 gctcattacc caggcaggcg tactcgagtg cgacggctgc tgagcccagg tatgccgcct
     4681 gcatgaggtt gccgccggcg gccaaggcaa gggttgtcgc ggcgtgcagc gcgccgtcgc
     4741 agccgagcga atcgacgagg tggtctgcca gcatggggag gtattcactc ctcaggcgtc
     4801 cgcgccactc gggccggtgg ttgtcctggg actgtcggct gaagaccacg aggccctttc
     4861 tgccgagggt cacgagcaat tctctcgcgt ttgtttcctg aaggatatcc caggcgacga
     4921 acgacaggcc gcgttcgaaa tcatgcatgg aggtccgcaa cgttcgctcg gcgggacaga
     4981 gcagatccgc gtccttgaag tcgagcagtc ggcctctggg actgccggca tcggcagtga
     5041 cgactggggc tttgggtttg gcgatctcgc ggatcctgcc gatcagtccg ccggtaatgg
     5101 ttccgtatcc gaagtcgctg atgatgatgg cgtcgatgcg gtcgatgatt gatttcaccc
     5161 actgggcggc cttttcttcg gcgagcgaat ccagcgggtg ataacggccg tgctcgacgc
     5221 gcatgacctt ggtggtgtcg acgaggtagc gggttttcct tggcaggtgg ggctgcgtgg
     5281 gcacccaatg ggtttcggca ccgatgcggg cgagcaattc ccgtatctgt cgggtggcct
     5341 cgtcggcggc gcaggcgctg aggaggtacg gtttggcgcc aaggccggcg acgtgctggg
     5401 cgatgatgcc ggttccgccg acgtagactc gctcttcgag ttgagtaagg gacatcatcg
     5461 gggcttcgcc ggccagatcg gtggcgtcgc agagcacgta gtggtcgagc aggacgtcgc
     5521 cgacgatgag tacgcgaagc gaggcgaaag aattgagcag tgcttcggtc gagttccgat
     5581 cgatttcgtg gcgccgacag attgtggtca ggcgatcgat gtccagggcc gggtcgcggc
     5641 tgagggcctc aatcaggcgg gtagaactga agacgacgtc cccgctgctg aacatgactc
     5701 gcccgccgta agcggagacg acctgtctct cggccagaaa gccggggtcg ctgctttgct
     5761 cgtattcgcg tcccttgacg tagacgtccg gtttgagttg tctgaggaga tccgtggcgg
     5821 tcgggtgatc attgacgtaa accaggtcga cggcttcgag ggcggcgagg ttttcggctc
     5881 gcagttcctg ggggatgtag ggtcgctgga gatccttgct gacctgcgag tcgccggtca
     5941 gcgagacgac gagcacgtct ccctgtcggc gtgcgaaatc gagatagcgc acgtgacccg
     6001 gatgaacgat gtcgaagcaa ccgtggcaat ggacgacggt tttgccggca gcgcgggctt
     6061 cggcaactct gtggagaagc tcgggcaggg tgacgatttt gctcatgccg tatcagtggg
     6121 ctaccgaggg tcaactctac cggcgtgctg tcacgcgcag ggaagccggt tgttggatcc
     6181 gacggtttcc tcttcttcta tcggcacttt gaacgccggc taggagggtt acggcggctt
     6241 gccgggtccg atgcgagcgt gacatggtct ggcgaaaggg ccgacgaggg cgtcggcccc
     6301 acttgtcgcc cgatccgtgg gtgacatggt ctggcggaaa gggccgacga gggcgtcggc
     6361 cccagttgtc gcccgat
//
LOCUS       DAOTPW010000296         6320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_10606_length_6320_cov_5.676137, whole genome shotgun sequence.
ACCESSION   DAOTPW010000296 DAOTPW010000000
VERSION     DAOTPW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10606_length_6320_cov_5.676137"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..2344
                     /locus_tag="P5316_19420"
     CDS             380..2344
                     /locus_tag="P5316_19420"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRR87185.1"
                     /translation="MAFFIPTAAAGNGLVLATFGKSGEIMGFFYPQIDFAQNIREAMP
                     AIRLPGQGNRFLWCFDESWRVAQSFEPSSNVLITRLAHREMDLSLEMTDVLPPNEHAL
                     LRRVVITKSSSVGPVQFVHYFKLAVGDALDRNCVFYYPQQNLTVQFLRDIAIAVGATE
                     AFRCRCGSYRDGRPSETKAAMAAGDYGAFDLSIGRVDFVIGFEPVVGDRWQTMLVMAG
                     GSSPETAMAAADRLTRVPFDEAVAAADRRVSEELSAAGTCPVPELADAFDRAVISLHD
                     LYSQSQGTFIAAPEFDPGYELSGGYGYCWPRDATVCALAAQDIGRGDMTRRFFEWCTH
                     TQMTNGHWYQRYWTSGSPGSVWCIHEDKIQLDQTCAILHAAGQFSRVLGSEAESFRES
                     FRPTAVAATRAILDYIGEDGLHCPSIDLWENSVGSFAYTQAGVIAALREADEVFGLEP
                     SRTGPAARGVLRDKLIKTFWQPDRQRWLRRITPEGHPDATLDSSAMGLIYPWAVLNLS
                     DPHDRGLAMATLNGIAQDLRSDVKGGGAILRFAGESYMGGGPGCVNTIWLALCRLLAA
                     RTAGSIDEKRQQIELAMEHLRVALANTSPTGQLPELIPKILFEYWAAPHAWACSLLIE
                     AVKVLRALVGERVAPFDAARARVRRRAPSH"
     gene            2509..3261
                     /locus_tag="P5316_19425"
     CDS             2509..3261
                     /locus_tag="P5316_19425"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRR87186.1"
                     /translation="MTRVVREFLLLAVVGVSVGLIANALNPNGLSLRRNYFTKPVVSR
                     GSARPYSGPGTNHGTQTGPASRAADTQMTASGGGGETGGRNTAKTGTDPSEQMTEDEM
                     VQRLRDLGFQALYHHEVVDIFSSPYRADGLYVIIDARTEQHYRSGHIPGAYHLDHYYL
                     DRTVGKVLEACRMADKIVVYCNGGVCEDSELAVGDLLERGIPFDRLYIYAGGFTMWAG
                     DGMQYEVGERNSGELVWDVPASGGQASEGAAK"
     gene            3258..3761
                     /locus_tag="P5316_19430"
     CDS             3258..3761
                     /locus_tag="P5316_19430"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRR87187.1"
                     /translation="MNSEPGLIRRLYRSGALMVFCRLAIGLLFVYMGFKKAMDPVTFL
                     KLVREYEMFPDNIWWLLNFTVATLPWVEILCGLLLLLGIAHRGTALLVMVMLSVFTPM
                     IWLRGLGIHDAEHIPFCRIIFDCGCGAGPVNFCWKMAENIALFLAAALILFSRIHRFY
                     LRVEFVR"
     gene            3970..5397
                     /locus_tag="P5316_19435"
     CDS             3970..5397
                     /locus_tag="P5316_19435"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR87188.1"
                     /translation="MFKRLAVCLSCMCCLDLTAPSLAQNTRATPAYRQMKSYLDAIPA
                     IDTHDHIRPFDRLSSDAQPGSNEEAMNLRALLQHCYYAWFNPLAPRPAGMSFDDWWSK
                     ARNNFDNGRATTFYRFLLPAFQDLYGVDFDRITDDQARELDRKIVENYRDQRWLYEVV
                     TERANIEVMVVDRYWAPLEMPSDYPFAVSVLNINSLIRGFHPSEYRDQHSDPYWYAKE
                     HGLKADSLDDYVGVLDAMIADAKQRGLPCLKNTLAYSRTLEFRNVAKERAAKAFGRPR
                     AELKPDEIRDFEDFVIWRICELAAKHDLPIQIHTGQARIQGSNPMLLVDLIEANPKTK
                     FVLFHGGFPWVGETGVIVMRHTSHVWVDSVWLPTLSYTMAKRAFHEWLEVMPSDRIMW
                     GADCGHAEGIYGATELTRRCVAEVLVEKVAAGDLTEEHARRIGRQILRENALTLFPVL
                     KQHLWKHKGKRMTPDPAATGSHGNQ"
     gene            5431..6276
                     /locus_tag="P5316_19440"
     CDS             5431..6276
                     /locus_tag="P5316_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87189.1"
                     /translation="MIGILPCAALLLIVGVPTVLLVILITRRGGRHVGEPACGKCGYA
                     VRGLPTFTCPECGSDLREAGIVTEGQSSMPPAVRGWLLVLIWTLILPIPAFLVTVLIG
                     HAAPRLDSTTARKTLEKPRSNAYQRIELVQHRVRRDNAIISNDLTIMLVPSQGELLEL
                     DFSSKDRHYAYRRQSGEKVQVDAGLDAMVVLDWMAQAGVDIEDQQVKSEAAELASMIQ
                     TASAGLVSDLPHRQFLSDGASSSSYVGPPQWVPVVAAVFWLAVWLSGCWRFWKIGARK
                     QGCTV"
BASE COUNT         1137 a         1794 c         2107 g         1282 t
ORIGIN      
        1 cgagcccggg cgccggcagt tcgcgtgcgg attggcccgc ggctgacggg gagtcgtgat
       61 gcttgagggg cagattgtgc gccttatcgg acttgggagc gattacgccc caaagccgat
      121 taacttgaag ggtcttcgat gccgataact aacaggctag aatgaagatg cggctgttgg
      181 caacgcggcg acggcgcgcg tctttccttg tcgccttcgg gaggtgtggt gccgcaaggc
      241 accggatcgc tggtggagtc ggcgacagtc gcgagccgag aggggcacag caacgagcaa
      301 tgcctgtcgt tgcggcctcg aaggggctgg ccgagcagct tcacgcaagc gccttcctcg
      361 gccgacctag gtggcagtaa tggcattctt catacccact gcggcagccg gtaacgggct
      421 ggtactcgcc acgtttggca agtccggcga gatcatggga tttttctatc cgcagatcga
      481 ctttgcccag aacatccgtg aggccatgcc ggcgattcgt ctacctggtc agggcaaccg
      541 cttcttgtgg tgtttcgacg agtcatggcg tgtggcccag tcgttcgaac cttcgagcaa
      601 cgtgctgatc acgcggctgg cacatcggga gatggatctg tcgctggaaa tgaccgacgt
      661 gcttccaccc aacgagcacg ctctgcttcg acgcgtggtg attaccaagt cttcgagcgt
      721 gggcccggtt caattcgtgc attatttcaa gctcgcggtc ggcgacgcgc tcgatcgcaa
      781 ctgcgtcttc tactacccac agcagaatct cacggtgcag tttcttcggg acatcgcgat
      841 tgccgttggg gccacggagg cgttccgctg ccgttgcggc tcgtaccgtg acggcaggcc
      901 atcggagacg aaggcagcga tggcggcagg cgactacggg gcatttgacc tgtcgatcgg
      961 gcgggtcgat tttgtgatcg gttttgaacc cgtggtgggc gatcgctggc aaacgatgct
     1021 ggtgatggcg ggcggatcaa gccctgaaac ggcgatggcg gccgcggacc ggctgacgcg
     1081 cgtgccgttc gatgaggccg tggcagcggc ggaccggcga gtttctgagg agctcagcgc
     1141 ggccggaacg tgtccggtgc cggaactagc ggacgcgttc gatcgggcgg tcatctcgct
     1201 gcacgacctg tacagccagt cacaggggac gttcattgcg gcgccggagt ttgacccggg
     1261 ctatgagcta tcggggggct acggctactg ctggccgagg gatgcgacgg tttgtgcatt
     1321 ggccgcgcag gatatcggcc gcggcgacat gacccgccgc ttcttcgagt ggtgcaccca
     1381 cactcaaatg accaacggcc actggtacca gcgatactgg acgagtggct cacccgggtc
     1441 agtgtggtgc attcacgagg acaagattca actcgatcag acctgtgcga tcctgcatgc
     1501 cgccgggcaa ttcagccggg tacttgggag tgaggcggag tcttttcgcg agagttttcg
     1561 tccgaccgcg gtggccgcca cgcgggcgat tcttgactac ataggcgagg atggcctgca
     1621 ttgcccctcg atcgacttgt gggagaacag tgttgggagt tttgcataca cgcaggcagg
     1681 cgtgatcgcg gccctgcgcg aggcagacga ggttttcggc ctggagccgt ctcgcacggg
     1741 gccggcggcc agaggggtgc tgcgcgacaa gctcatcaag accttctggc agcccgatcg
     1801 ccagcggtgg cttcgccgaa tcactccgga ggggcaccct gatgcgacgc tcgactcttc
     1861 ggccatgggg ctgatttatc cgtgggcggt cctgaatctg agtgatcccc acgaccgtgg
     1921 gctggcaatg gccacgttga acggtattgc ccaggatctg cgttcagatg tcaaaggggg
     1981 cggagccatt cttcgatttg cgggtgagtc ttacatgggc ggagggccgg gctgcgtcaa
     2041 caccatctgg ctggcgctat gccggctgct cgcggcacgg acggccggca gcatcgacga
     2101 gaaacggcag cagatcgagc tggcaatgga gcacctgcgg gtcgcgttgg ccaacaccag
     2161 tcccacaggt caactgccgg aactgattcc gaagattctc tttgagtatt gggccgctcc
     2221 acacgcctgg gcgtgttcgc tgctgatcga ggcggtgaag gtgctgcgcg cactggtcgg
     2281 cgagcgtgtg gcccctttcg acgccgcccg cgcccgcgtt aggaggcggg ctccttcaca
     2341 ctgatcggtt atcggcatgc tgaggcgaaa gcccgattgc aaagctctct cgccggagcg
     2401 attcccccgg atataatgca ggtgcggagg gtgcgggcgc gctgtgccgc gttgagggca
     2461 ttggcgatcc gcgaccagag gcccaatccg gtgtcgacga tgaaggaagt gacaagagta
     2521 gtacgcgagt tccttcttct ggccgtcgtc ggggtgagcg tcggcctgat tgccaacgcg
     2581 ctcaatccga acgggttgtc gctcaggagg aactacttca ccaagccggt cgttagcaga
     2641 ggctccgccc gcccgtacag cggtcctgga acgaaccacg gcacgcagac aggtccggcc
     2701 tctcgagcgg cggacaccca gatgacagcc tccggcggcg gaggtgaaac ggggggccgc
     2761 aacacggcga aaacgggcac agacccgtcg gagcagatga cggaagacga aatggtccag
     2821 cggctgagag acctcggttt ccaggccctg taccaccatg aagtggttga catcttcagc
     2881 agcccctatc gggccgacgg tctgtacgtc atcatcgacg cccgtacgga gcagcactat
     2941 cgcagcggcc acattccggg ggcctaccac ctggatcact attaccttga tcggaccgtg
     3001 ggcaaggtgc tggaggcatg cagaatggcg gacaagatcg tggtctactg caacggcggc
     3061 gtttgcgaag acagtgagct ggcggttggc gatctcctgg aaaggggcat cccgttcgac
     3121 aggctgtata tctatgcggg cggctttacg atgtgggcgg gtgatggaat gcaatacgag
     3181 gtcggggagc gcaacagcgg cgaactggtc tgggacgtgc ctgcgagcgg cgggcaggcg
     3241 tccgaagggg cagcgaaatg aactctgagc cgggcctgat tcggcgactc tatcgcagcg
     3301 gcgcgctgat ggttttttgc cggctggcca tcggcttgct gttcgtttac atgggcttca
     3361 agaaggccat ggatccggtg actttcctca agcttgtccg cgagtacgaa atgtttccgg
     3421 acaacatctg gtggctgttg aacttcactg tcgccaccct gccgtgggtg gaaatcctgt
     3481 gcgggctgtt gttgctcctg ggcattgccc accgcggcac cgccttgctg gtgatggtca
     3541 tgctgtcggt ctttacgccg atgatctggc tgcggggcct gggcatccac gatgccgagc
     3601 acatcccgtt ctgccggatc atcttcgact gcgggtgcgg ggcgggcccg gtcaacttct
     3661 gctggaagat ggctgagaac attgccttat tcctggcggc cgcgttgatt ctgttctcgc
     3721 gaatccatcg gttttatctg cgcgtcgagt ttgtccggta agacccttcg ggccgggtgc
     3781 tgttgtcggt gagtcgagtg ttgcagcggc ggccggccgc ggcaaccccc ttcgaggagg
     3841 cgaaatccgc cgaccttgtc ggtcgctcat ctcgcccggg acggcgaggg tatcatgggc
     3901 ggtcgcaagc acttgcagga ccgattccgg catggacaat gaagccggcc tgatgcaaag
     3961 gagtaaccga tgttcaagcg actcgccgta tgtctgtcat gcatgtgctg cctcgacctg
     4021 accgccccat cgctcgccca gaatactcga gcgactccgg cctatcggca gatgaagtct
     4081 tacctggatg ccatcccagc catcgacacg catgaccaca tccggccgtt cgatcggctg
     4141 agctcggatg cccagccagg ttccaatgag gaggcgatga accttcgtgc tcttctgcag
     4201 cattgctatt atgcgtggtt caatccgctg gcgccgcgtc cggcggggat gtcgttcgac
     4261 gactggtgga gcaaggccag gaacaatttc gacaacggcc gggcgaccac tttctatcgc
     4321 ttcttgctgc cggccttcca ggacctttac ggcgtcgatt tcgaccggat cacggacgac
     4381 caggcccgcg aactggaccg caagattgtc gaaaactacc gcgatcagcg ctggctctac
     4441 gaagtggtta cggagcgggc caacatcgag gtcatggtgg tggatcggta ttgggcgccg
     4501 ctggagatgc cttcggatta tccgtttgcg gtttcggtgc tgaacatcaa cagcctgatt
     4561 cgggggttcc atccctcgga ataccgcgac cagcattcag atccgtattg gtacgcaaag
     4621 gagcacgggt tgaaggccga ttctctcgac gactatgtcg gcgtgctgga cgcgatgatc
     4681 gctgacgcga agcagcgcgg gctgccgtgc ctgaagaaca ctctggctta ttcgcgaacg
     4741 ctggagttcc gcaacgtggc caaggagcgg gcggcaaagg ctttcggccg gccccgggcg
     4801 gagctgaagc ccgatgagat tcgcgatttt gaggatttcg tgatctggcg aatctgcgag
     4861 ctggccgcca agcacgacct gccgatccag atccacaccg gccaggcccg catccagggc
     4921 tcgaatccga tgctgctggt cgatctgatc gaggccaacc cgaagacgaa gttcgtcttg
     4981 tttcacggcg ggtttccgtg ggtcggcgaa accggcgtga ttgtgatgcg tcacacgtca
     5041 cacgtgtggg tcgattcggt ctggctgccg acgctgagct acacgatggc caagcgggcg
     5101 tttcacgaat ggctcgaagt catgccgtca gaccggatca tgtggggcgc cgattgcggt
     5161 catgcggagg gcatttacgg agcgactgaa ctgacgcggc gttgcgttgc cgaggtgctg
     5221 gtcgagaagg ttgcggccgg cgatttgacc gaagagcacg ccaggcgaat cggccggcag
     5281 attctccgcg agaatgccct gacgcttttc ccagtcctca agcagcatct ctggaagcac
     5341 aaaggcaaga ggatgacgcc ggacccggcc gcgacaggat cgcacggcaa ccagtaggca
     5401 tgttgatgtt ttcccgagcg ggaggcggat gtgatcggga ttctgccatg tgcggcgcta
     5461 ttgcttatcg tgggcgtgcc gacggtactg ctcgtcattc tgatcacgag gaggggaggc
     5521 cggcacgttg gtgagccggc gtgcggcaaa tgcggttacg cggtgcgcgg cttgccgacg
     5581 ttcacttgcc ccgagtgcgg ctcggatctg cgcgaggcgg ggattgttac ggaagggcaa
     5641 tcctcgatgc cgccggccgt gcgcgggtgg ctcctggtgc tgatctggac tttgatcctg
     5701 ccgattccgg cctttctggt taccgtcctg atcggccatg cggccccgcg gctcgattcg
     5761 acaacagcac gaaagacgct cgagaagccg cggtcgaacg cctatcagcg aattgaactg
     5821 gttcagcatc gggtcaggcg agacaacgcg ataatctcca acgatttgac gatcatgctt
     5881 gtgccttctc agggtgaact ccttgagttg gatttttcct ctaaagaccg ccattacgcc
     5941 tatcggcgac agtccggaga aaaggtgcag gtcgacgccg gtcttgatgc gatggtggtt
     6001 ctcgactgga tggcccaggc gggcgtcgat attgaagacc agcaggtcaa gagcgaagcc
     6061 gcggaattgg cctcgatgat ccagaccgct tccgccggcc tggtgagcga tctgccgcac
     6121 agacagttct tgtccgacgg agcgtcttca tcttcatacg ttggtccgcc acaatgggtg
     6181 ccggtcgttg ccgcggtctt ctggctcgcc gtctggttgt cggggtgttg gaggttctgg
     6241 aagatcggcg ccaggaagca gggatgtacg gtttagccat tttacgctgc cgcgcagagc
     6301 gtgaattgca gcgtattcaa
//
LOCUS       DAOTPW010000297         6239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_10770_length_6239_cov_4.260026, whole genome shotgun sequence.
ACCESSION   DAOTPW010000297 DAOTPW010000000
VERSION     DAOTPW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10770_length_6239_cov_4.260026"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2612
                     /locus_tag="P5316_19445"
     CDS             <1..2612
                     /locus_tag="P5316_19445"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRR87190.1"
                     /translation="GYLAILGVPAVLPAAEVIVDNTDAGFTALSGVWSTRTSGTGEYG
                     MNYRRATTATTASGEVQWQPTIPASGDYEVFVWYPSGDGTHITDARYTVHYNGGSQTF
                     LVNQQTNTGQWVSLGTFEFLEGSSANGRVSLTNQSATAGARVYADAVRFYKPEPVSLT
                     MAVSPAGAGTTTPAEGGPYGYLEGDVVSIAASPASGYRFSHWTVSAGSDVADPTSADT
                     SVTMDVDKTVTAVFAEIRPEFRGFWVDVFHVGMQNAAQIDQMISLAVQGNYNAIVPEV
                     LAFHDNEVGSHGAYWKSDIVARSVYVTESFDPLAYMVQQAHASGLQVHCWLVAFRVSS
                     KWPPPGNAFLAAHPEWLKVPLANMGTVAKVGSYYEFDPGSPDVQDYLASIVRELVTDY
                     EIDGIHWDYIRYTNRDSGYPADTSYENSSLKRFQRIYNRTDVPSPTGDTQWDDFRRRT
                     VTEVVRRMTWEIPLITSNPRQPVRYSSAVVTWHPCSTDFHNTRPYYEVFSDWEDWQSK
                     AYLDSPVLMAYFDEDGSYAQTYRDWVDNSINLWRYNRQTIIGPGIYMNSFANSVVQME
                     YARAAGADGLCTYSYATTNDAGLTWTDWYPYVAENFFHYPVPVPPMPWKDPATATEGI
                     LYGRVTDGSTGLPVDDATVQVGALPSVQTDAGGYYIVTNITAGASGTYYSVTAGKDGY
                     PVVTHPSVQVIAAGVRRDDLVLGGTPPAITRHPAPQNVCPGGTATFTVSATGDMPLSY
                     LWQKNQSDLSDGGHYSGVTTATLTVSSADADDAGDYRCVVSNGSGSATSEEAGLTLKP
                     LVGADFDADCDVDADDYDAFEICASGPGVSVLAGCEGKDFDADNDVDQADFSVFQRCM
                     SGENTAPDPACAN"
     gene            3541..4965
                     /locus_tag="P5316_19450"
     CDS             3541..4965
                     /locus_tag="P5316_19450"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRR87191.1"
                     /translation="MIWLKRLLVITAIGLSLFGAYLSTQMLLHHLFGKSSSSLLTAMC
                     DAASGGCDRIASSRWSTVPPRPKKGEQDGARSSDGKAATPSHGGIRVPVPILGLLYFS
                     FLAAWFLGVGCPNELGRRWQLVPFVAVLAGNGWSIFFMYIMARVVKAWCAGCLTIHAI
                     NFLLLVIVLVTCPRRWRKDAAYALRGDCPGSGAIPAAPHPTARLALVTLALAVSFGVI
                     GGAIAVAAVFALEVAKNKGIVDEVVGDPEILARMYLKRDKMEIPISPDAPFRGGPADA
                     PLTMIVFSDLTCSRCKEFEDLLTKTILPRSDGRLRVVFKHFPLSDGCNQYAPGRGSPH
                     SCEAARAVEAARLQGGNELFWRMLAEVRAWSEDLASLDYRAEAVKLGLDGDRFVADMQ
                     SEAVRNRIREDVELGHRLAVDATPTVFLNDRIVPSVAQDKPAFWELMCERLGGEPAVV
                     ERSVTSTSTAPSLTKAESLPGPPR"
     gene            complement(5059..>6239)
                     /locus_tag="P5316_19455"
     CDS             complement(5059..>6239)
                     /locus_tag="P5316_19455"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRR87192.1"
                     /translation="GRAILEGMVSGGTPPYFIEWSPAEGLDNPEALQPTASPAVTTTY
                     TLTVTDAMSVQAADTVLVNVTDPGIPADMDGDGDVDQADFGLFQVCISGSSVPQTEPA
                     CRGAKLDDDDDVDADDVSLFTRCVSGAGVPGDRQCAGPFPPAITLHPISQSIAAGESV
                     VFTVQAAGSAPLSYQWQKDLVNLNNGGHYSGANTSTLSITNADGNDAADYRCVVTNAH
                     GSATSNAATLKVTSVSPSEGCFTNNDLETFISGVAAGWTSAGSSASYSASTDAYSGSY
                     SQEIKWTSAGAKMSVIYQQVWLKVGVPYTIRAYFRMNDTSRVNGLIRVDYNGGTDPNI
                     YDLSTSAPRLEWGSKSLTFTRTTGSDGWATVFVGGYGSLVHSNDWCRIDLITPACAAN
                     "
BASE COUNT         1109 a         1787 c         2104 g         1239 t
ORIGIN      
        1 cggggtatct tgcgattctg ggtgtcccgg ctgtgttgcc ggccgcggaa gtgatcgtgg
       61 ataacaccga cgccggcttc actgcgttgt ccggggtttg gagcacgcga acaagcggga
      121 ccggcgaata cggtatgaat taccggcgtg cgaccacggc cacgacggcc agcggggagg
      181 ttcagtggca accgaccatt ccggcaagcg gtgattacga ggtgttcgtg tggtatccgt
      241 cgggagacgg cacgcatatc accgacgccc ggtacacagt tcactacaac ggcgggagtc
      301 agacttttct cgtcaaccaa cagacgaaca cggggcaatg ggtctcgctc ggcacgttcg
      361 aattcctgga gggctcgtcg gccaacgggc gcgtcagcct gacgaatcag tcggcaaccg
      421 ccggggcgcg ggtttatgcg gacgccgttc gcttttacaa acccgagccg gtcagtctca
      481 cgatggcagt gagtccggcc ggggcgggta cgaccacgcc ggccgagggc gggccatacg
      541 gctacctgga gggcgacgtg gtttcgatcg cggccagtcc ggcgtccggc taccggttca
      601 gccattggac ggtctcagcc ggttcggacg tcgccgatcc gacgtccgcc gacaccagcg
      661 tgaccatgga cgtggacaag acggtaacgg cggtgttcgc ggagattcgg ccggaattcc
      721 gcggtttctg ggtggatgtc tttcatgtag ggatgcagaa cgccgcccag atcgatcaga
      781 tgatcagtct ggcggtgcag gggaactaca atgcgatagt accagaagtg ctggctttcc
      841 acgacaacga ggtcggctca cacggggcat actggaagtc cgacatcgtg gcccggtcgg
      901 tctatgtgac ggaatccttt gatccgctcg cctacatggt tcagcaggct cacgccagcg
      961 gactccaggt ccactgctgg ctggtggcct ttcgggtgtc gtcgaaatgg ccgccgccgg
     1021 gcaacgcctt tttggccgcc catccggagt ggctcaaggt tccgctggcc aacatgggga
     1081 ccgtcgcaaa ggtcggcagc tactatgagt ttgaccctgg ttcacctgat gtccaggatt
     1141 accttgcctc gatcgtgcgc gagttggtga ccgactacga gattgacggc atccactggg
     1201 actacatccg ctacacgaac agggattccg gttatcctgc ggacacgagc tacgaaaact
     1261 caagcctcaa gcggttccag cgaatctata accggaccga cgtgcccagt cccacgggtg
     1321 acactcagtg ggacgatttc cggcgccgca ccgtgaccga ggttgtacgc cggatgacct
     1381 gggagatccc gctgatcacg agcaacccgc gccagccggt ccgttattct tcggcggtcg
     1441 tgacgtggca tccgtgcagc accgattttc ataacacacg gccctactac gaggtcttca
     1501 gcgactggga ggactggcag tcaaaggcgt atctggactc gcccgttctg atggcctatt
     1561 tcgatgaaga cggttcctac gcgcagacct atcgcgactg ggtggacaac agtatcaact
     1621 tgtggcgata caaccgccag accatcatcg gacccggcat ctacatgaac tcgtttgcca
     1681 acagcgtcgt tcagatggag tacgctcggg cggcgggtgc ggacggtctg tgtacgtatt
     1741 cgtatgccac gaccaatgac gcggggctta cgtggaccga ttggtatccg tacgtggcgg
     1801 agaacttctt tcactatcct gttccggttc cgccgatgcc ctggaaggat ccggcgacgg
     1861 cgacggaagg gatactctac gggcgagtca ctgacggcag taccgggctg ccggtggacg
     1921 atgcgaccgt tcaggtgggg gcgctcccga gcgtgcagac cgacgccggc ggttattaca
     1981 tcgttacgaa catcacggct ggtgcctcgg gaacatatta cagcgtgacg gccggcaagg
     2041 acggctaccc ggtcgtgacg catccaagtg tgcaggtcat tgccgccggt gttcggaggg
     2101 acgacctggt actcggcggc accccgcctg cgataacccg gcacccggcg ccccagaacg
     2161 tctgcccggg cggcaccgcc acgttcacgg tatcggcgac aggggatatg cctctttcat
     2221 atctgtggca gaagaatcaa agcgacctga gcgacggcgg tcactactcg ggcgtgacga
     2281 cggcgacttt gacggtttcg agtgccgacg cggatgacgc gggtgactac cgttgcgtgg
     2341 tgagcaacgg gtccggcagc gccacatcgg aggaggcagg gttgacgctc aaaccgcttg
     2401 ttggtgcgga tttcgacgcc gactgcgacg tggatgcgga cgattatgat gcgttcgaga
     2461 tctgcgcgtc ggggcccggc gtatccgttc tggccggctg cgaggggaag gactttgacg
     2521 cggacaacga tgttgaccag gccgatttca gcgtctttca gcgatgcatg agcggcgaga
     2581 acacggcgcc ggatccggcc tgcgctaatt gaatcccagc gttcagcgca agacgggggc
     2641 ctgcccgaag agaagaagac gcaccgttgc tcgtggacgc cgtgtcaagc tggcgttcag
     2701 cgggcggtgg cgccgctttt cgggcaggcc ctggaagtcc ctgttgaacc tctctcccgt
     2761 gcccgccggt tacgaggcgc cgccaaaaat cgcgcccagt gcctgaaaga tcgccaggag
     2821 catcgcgata atgctatatg gacacatgca agaaacctcc acagtttggg aaccccacaa
     2881 gcagccgccg atcttcccag cgagtcgggc ggccggcgaa gaatccgggc gacggtgaga
     2941 ccctcgcccg gccgtttggc ctgacacaag ccgtttaatt catcggccgc cagggaagat
     3001 ctcctcgaca aaatctgttc atggcgcgga tgctttgaag cggccgtcgc cggtgggggg
     3061 gagtcgcgag tgcagaagac attgaggtcg tcacacaaac gatacaagcg cctttggccg
     3121 gtgacaagat gtttgcccgc gtcgaccggc tataccgtcc attcatgcgt gatgtgaagg
     3181 cagtgaccgt gacgcatttc gcagggtcat attccgtttg ccacgggggc ttttccgcct
     3241 gcacagaccc ggagcggcag aaggcactgg cgatttcccc gcctgtaccg tcacgaaacc
     3301 ggcagagagg cggccggcgg cacccttggc ccgactgtga cggacgcctt ggggcgtatt
     3361 gtcccatggc ccccttgtat cgtaccgcct gcgtgagata atccgccctt ccgcggaggt
     3421 cgggaaggcg tattccctgg agtcggctat cggggaaatc cgagcgggct gtgcaaggtg
     3481 tggaatcggc ggccgccggg gtttgcgggc ccccgcgcag cggggacaag gagaaagccg
     3541 gtgatctggc tcaaacggtt gctcgtcatc acggccattg ggctttcgct gttcggggct
     3601 tacctgagca ctcaaatgct gctccaccat ctatttggaa agtcgagcag cagtctgctg
     3661 acggcgatgt gtgatgcggc gagcggaggc tgtgacagaa ttgcctcgag ccgttggagc
     3721 actgttccgc cgagacccaa gaagggggag caagacggcg cgcggtcgag tgacggtaag
     3781 gcggcgacgc ccagccacgg cggcatccgc gtgcccgtgc ctattctggg tttactgtat
     3841 ttttcttttc tcgctgcctg gttcctggga gtgggctgcc ccaacgaatt ggggcggcgg
     3901 tggcagttgg tgccgttcgt cgcggttctg gcgggaaacg gctggtccat cttcttcatg
     3961 tacatcatgg ccagggtagt caaggcgtgg tgcgcaggct gcctgacgat tcacgcaatc
     4021 aacttcttgt tgctggtgat cgtgcttgtg acatgccccc gtcggtggcg aaaggatgcc
     4081 gcttacgcac tgaggggtga ctgcccgggt tctggagcga tacctgcggc accgcatccg
     4141 acggctcggc tggcgctggt gacgctggct ctggccgtga gtttcggggt gatcgggggg
     4201 gcgatcgccg ttgcggcggt tttcgccttg gaggtggcca agaacaaggg tattgtcgat
     4261 gaggttgtcg gggatccgga gatacttgcc cggatgtact tgaagcggga caagatggag
     4321 atacccatct cgccggatgc accttttcgt ggcgggccgg ccgacgcacc actgacgatg
     4381 atcgtattca gcgacttgac ctgctcaaga tgcaaggaat ttgaggatct gttgaccaag
     4441 accattctgc cgcgtagcga cggtcggttg cgcgtggttt tcaagcactt tccgctttcg
     4501 gacggctgca accaatatgc cccgggtcgt ggttcgccgc attcgtgtga ggcagcgcgt
     4561 gctgtggagg cggcccggct tcaggggggc aacgagctgt tctggaggat gctggccgag
     4621 gtgcgtgcct ggtccgagga tctcgcttcg ctcgactatc gggccgaggc ggtgaagctg
     4681 gggcttgacg gcgaccggtt tgtcgcggac atgcaaagcg aggcggtgcg gaatcgcatt
     4741 cgcgaggacg tggagttggg ccacaggctg gcggtggatg ccacgccgac cgtttttctg
     4801 aatgatcgaa tcgtgccctc tgttgctcaa gacaagcccg ccttttggga gctcatgtgt
     4861 gaaaggctcg gcggcgaacc ggcagtcgtc gaacggtcgg ttacttcgac ttcgacggcc
     4921 ccgtcgctga ccaaggctga atccttgccc ggtccgcctc gttgaagcag taccggatca
     4981 gcgaaggcgt cggtgagctg atgtctgccg caccgtcaac aacgaccaac ctccgagtcg
     5041 attgttctta cgttccactc agttggccgc gcaggcgggg gttatgaggt cgatgcggca
     5101 ccagtcgttg ctgtgcacga ggcttccgta gccgccgacg aagacggtgg cccatccatc
     5161 ggagccggtt gtcctggtga atgtcaggga cttgctcccc cattcgagcc gcggagcgct
     5221 tgtcgagagg tcgtagatgt tcgggtctgt gccgccgttg tagtccacgc ggatcaggcc
     5281 gttgacgcgg ctggtatcgt tcatccggaa gtaagcccgg attgtgtacg gtacgcccac
     5341 tttcagccag acctgctgat agatcaccga catcttggcg ccggcggagg tccatttgat
     5401 ctcctgtgaa tacgagccgg agtaggcgtc ggtactggcg gagtaggagg cgctgcttcc
     5461 ggcgctggtc caaccggcgg cgacgcccga gatgaaggtc tcgaggtcgt tgttggtgaa
     5521 gcagccttca gacggagaga cggaggtgac tttgagcgtt gcggcgttgg acgttgcgct
     5581 gccgtgggca ttggtaacga cacagcggta gtcggctgcg tcgttgccgt cggcgttggt
     5641 gatggacaag gtgctggtat tcgcgccgga gtagtgcccg ccattgttga ggttgaccag
     5701 gtctttttgc cactggtagc tcagcggggc gctaccagcc gcctgcacgg tgaagacgac
     5761 agactcgccg gccgcaatgg actggctgat cgggtgtagc gtgatggcgg gcgggaaggg
     5821 gcctgcgcat tgccggtccc ccggaacacc ggcgccgctg acacagcggg tgaaaaggga
     5881 cacgtcgtcc gcgtccacgt cgtcgtcgtc gtccagtttg gcgccccggc aggcgggttc
     5941 ggtttgcggc acgctggagc cgctgatgca gacctggaag aggccgaagt cggcttgatc
     6001 cacgtctccg tccccgtcca tatccgccgg gatgcccggg tcggtcacgt tgaccagcac
     6061 ggtatcggcg gcctgcacgc tcattgcgtc ggtgacggtc agggtgtaag tagtggtgac
     6121 ggcgggcgag gcggtgggct ggagcgcctc gggattgtcc agtccctctg ccggtgacca
     6181 ttcgatgaag tacggggggg ttccaccgct taccatgcct tccaggatag cccggccgc
//
LOCUS       DAOTPW010000298         6081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11108_length_6081_cov_4.904912, whole genome shotgun sequence.
ACCESSION   DAOTPW010000298 DAOTPW010000000
VERSION     DAOTPW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11108_length_6081_cov_4.904912"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="P5316_19460"
     CDS             complement(<1..549)
                     /locus_tag="P5316_19460"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87193.1"
                     /translation="MKRSPVSRREFLNRSVVAAAGVAAASLARPAPLRAAGADDRIVI
                     GFMGCGGRGNFLVKEMAARKNLDFAYMCDPDAGRLAAGIKQAEKLTGKAPKAVKDYRE
                     ILDDKNVHALFVCTPDHWHALPTIHACQAGKDVYVEKPASHSVWEGRKMVEAARKYGR
                     VVQLGTQTRSGRYTREAVEYIRS"
     gene            complement(704..2215)
                     /locus_tag="P5316_19465"
     CDS             complement(704..2215)
                     /locus_tag="P5316_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRR87194.1"
                     /translation="MSVLIPLVAVFLGPLAASETPAGSAPSPQTAARFEPTWESLERY
                     QVPQWYLDGKFGIFIHWGVYSVPAFGNEWYPRNMYLQDSPEFKHHLATYGPHSQFGYK
                     DFIPLFKAEKFEPARWAELFRKAGAKYVVPVAEHHDGFAMYDCSLSPWCAAKIGPKRD
                     IIGELAEAVRKEGMVFGLSSHRAEHWWFFDGGMRFDSDVKDPTFAGLYGPAQPEKTSR
                     PDKAFLDDWLARTRELVDKYQPQLVWFDWWIQEPEFEPYLREFAAYYYNKDAEWQKGV
                     VINYKFKAFPDKAAVLDVERGQLAEIRPFFWQTDTSISRNSWGYVSRQDYKTVDSLID
                     DLVDIVSKNGALLLNIGPKPDGTIPEAEEKILLEIGRWLEINGEAVYGTRPWKVYGEG
                     PTKIIEGSMNDTKRSAFTGLDIRFTTKGDVLYATVLAWPEGDRVIIRCLARDSSLYRG
                     EISDVKLLGCRDPVVWERTAEGLAVRLPATKPCEHAFVLRISPAERAAQRPAK"
     gene            complement(2322..4148)
                     /locus_tag="P5316_19470"
     CDS             complement(2322..4148)
                     /locus_tag="P5316_19470"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRR87195.1"
                     /translation="MKKTCTMSIAAISTWILFVPSWSLGQNVPTTQAVETMSGLLGRV
                     PADAAEVLPGLSKACPLDLGRTSIAEAILEAAKSGSAEIPVTTYTQYREFRKSGDREP
                     YQKPYFEKRALLAKAVLAAWLQGDLASVDFVNDLVWNICEETNWVIPAHESVGEIDLM
                     AAETAATLAFTELLVGPRLPDEIRTRLREEVKRRVIEPYLDHGAKFWWDNGANNWTGV
                     CAGSIGQVLLILEPDPERQARGLALVVDQLNRFVDRAFEEDGASTEGIGYWNYGLAHF
                     VIFAEMLRARTGGAIDLLAGEKIARIAGYPTAVAIGRHAFASFSDSSENASVLPFIAA
                     RLADRTGRPALLAQASDKAHEGRLGWVLCNLIWHGDQPRREPLFENVVLPKAGVARCV
                     GKAGDRSVILVAKAGNNAEQHNHNDVGSFVLRIGETTFLCDPGAGLYNAAYFGRRRYE
                     NVFASSYGHSVPRIGGMQQKPGGQYRRTLEVSSDGTARIDMHEAYGIAQLKSFQRTFK
                     IDPDGRVTITDELRFDGDGMEVEEAFVTWHDVKVNGPVAHVVGDESMLEIRARSGVFK
                     AERLEDACKANKKKGVLTRLSASYPQAPIVKTHFMFVYKKAT"
     gene            complement(4145..5458)
                     /locus_tag="P5316_19475"
     CDS             complement(4145..5458)
                     /locus_tag="P5316_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87196.1"
                     /translation="MARDIDRRTILKRGLGALAGTLAATGVRQARTAEPVASAPANLN
                     GWHKHGIVLDATEPWEQNGIQSFTSPSEPLDGDRWRIWYSAVGTHETVKYTIAYAEGV
                     PGQEMKKYPVQCSTGEPGDGAFTLGPLPEKWRPVQVVHIPLKNGKHRIYFWAHGPGIL
                     RYLAADSEDGHRYRVIDPLRPVLYHFHDRAAWGVASPDGVMLHKEPSRDRPADEPLAP
                     SHLLSNDATNIYQLPDGTFEMYSVALIQVPKDDPAYMPHDNAPGLIRLVDRYVSDDGL
                     HFEKRRRVIDRDAKDPPDQQFYYLSVTHTPKGRIGMLGHYRCQAQTMDLEWCYSEDGV
                     RWDRPARKAWIPRGDQTQPDSYGIYAPSGLVRRGGRYHLFYTAVNSAHNGKDSYGPPR
                     CLIMHATIDDIARPQASALRDAMPRNSESEIEKWGGDGRRGCRNS"
     gene            5647..>6081
                     /locus_tag="P5316_19480"
     CDS             5647..>6081
                     /locus_tag="P5316_19480"
                     /inference="COORDINATES: protein motif:HMM:NF018606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87197.1"
                     /translation="MTRTPVFWGFLSVSMSAMTVAIANPVSDEETTPGKYLEAVQTFA
                     DNVLRHGKDVYGSKHTPLFADGLNIDTHEPVIWKLPKEQAEKWRMPTEWTLSNLASQQ
                     NLFRVFVALTDLTGDQKYKRAAVGATRYALDHLRHENGLLFWG"
BASE COUNT         1157 a         1898 c         1793 g         1233 t
ORIGIN      
        1 ggaacggatg tactccacgg cctcacgtgt gtatcgaccg ctgcgcgtct gggtgccgag
       61 ctggacgaca cggccatact tccgggcggc ctcgaccatc ttgcggcctt cccagacgct
      121 gtgggatgcg ggcttttcga catagacatc cttgccggcc tggcaggcat ggatcgtcgg
      181 cagcgcgtgc cagtgatcgg gcgtgcagac gaacaaggcg tggacattct tgtcatcgag
      241 aatctcgcgg tagtccttca ccgccttggg ggccttgccg gtaagctttt ctgcctgctt
      301 gatcccagca gccagacgac ccgcgtcggg gtcgcacata tacgcgaaat cgaggttctt
      361 gcgggcggcc atctccttaa ccaggaagtt gccgcgtccc ccgcagccca tgaagccgat
      421 gacgatcctg tcgtcggccc cggcggcccg taatggtgcc ggacgggcca acgaagccgc
      481 ggcgaccccc gcggctgcga cgaccgaacg gttcaggaat tccctccgac tcacgggact
      541 ccgtttcatt tgtatcactc ctctccatca cctggatggt ttcagcccgg ctgatctcag
      601 attgaatcgt tgcctgccta ggcacttcag ggacgcattg gcgtgcgaac cgtcggtaga
      661 atccgccgga tctcaacctc atcggccgct gaccaagccg cctctacttt gccggccttt
      721 gagcagcgcg ctcagcggga gagatgcgca gcacaaaggc atgttcgcat ggctttgtcg
      781 ccggaagcct gacggcaagt ccctcggcgg tccgttccca gacaaccgga tccctgcaac
      841 ccagcagctt gacgtcactg atctcgccgc ggtatagcga tgagtccctg gccagacagc
      901 gtatgatgac tctgtcaccc tccggccagg ccagcaccgt agcgtagagc acgtcgcctt
      961 tggtggtgaa acgaatgtcc agcccggtga acgcacttcg cttggtgtcg ttcatggaac
     1021 cttcaatgat tttcgtcgga ccttcgccgt aaaccttcca cggacgcgtg ccgtaaactg
     1081 cctcgccgtt gatctccaac caccggccga tctcaagcaa tatcttctcc tcggcctcgg
     1141 ggatcgtgcc gtcgggcttc ggcccgatgt tgagcagcag ggccccgttc ttgctgacga
     1201 tgtcgaccag atcgtcgatc agagagtcaa ccgtcttata gtcctgtctg ctgacatatc
     1261 cccacgagtt cctggagatc gaggtatccg tttgccagaa gaaaggcctg atctcagcca
     1321 gttggccgcg ttcgacgtca agcacggccg ccttgtcggg aaaggctttg aacttgtagt
     1381 tgatcacaac gcctttttgc cattcggcat ccttgttgta gtagtacgcc gcaaactccc
     1441 gcagatatgg ttcgaactcc ggctcctgga tccaccagtc gaaccagacc agttgcggct
     1501 gatacttgtc gaccagctcg cgcgtgcgag ccagccagtc gtcgaggaag gccttgtccg
     1561 gccggctggt cttttcgggc tgggccgggc cgtacaggcc ggcgaacgtg gggtccttca
     1621 cgtcggaatc gaatctcatg ccgccgtcaa agaaccacca gtgttcggca cgatggctgg
     1681 acagtccgaa gaccatgccc tctttcctaa ccgcctctgc cagttcacca ataatgtctc
     1741 gtttaggccc gatcttggcg gcacaccacg gcgacaggct acagtcgtac atggcaaagc
     1801 cgtcgtgatg ctcagccaca ggaacaacgt atttggcgcc ggcctttcgg aagagctcgg
     1861 cccagcgagc cggttcgaac ttctccgcct tgaacaaggg gataaaatcc ttgtagccga
     1921 actgcgagtg ggggccatat gtggccaggt ggtgcttgaa ctcaggcgag tcctgcaggt
     1981 acatgttgcg gggataccac tcgttgccga aagcgggcac ggagtagacg ccccagtgaa
     2041 tgaaaatgcc gaacttgccg tccagatacc actgcggaac ctggtagcgt tcgagcgact
     2101 cccaggtggg ttcaaatcgg gcggccgttt gagggctcgg cgccgaacca gccggcgtct
     2161 cactcgctgc aagaggcccc aggaagaccg caaccagcgg aatcagcaca gacatgattc
     2221 tgtctcgcct ccgaatgtag gacttcaacg cgcgcccaca caggttctga aacaatgaca
     2281 gcttatcgat tcggcggcat ttggcaagga cttgtcaacg gttatgttgc ttttttgtag
     2341 acgaacatga aatgggtctt cacgatcggg gcttgcgggt aggaggcgct cagccgtgtc
     2401 agaacaccct ttttcttatt cgccttgcag gcgtcttcga gccgctcggc cttgaacacc
     2461 ccggaacgag cacggatctc caacatgctt tcgtcgccga ccacatgggc caccggaccg
     2521 ttcaccttga cgtcgtgcca ggttacgaaa gcctcttcaa cctccatgcc gtcgccgtcg
     2581 aagcgcaact catccgtgat ggtcacccgg ccatcaggat cgatcttgaa cgtgcgctga
     2641 aagctcttga gttgagcgat gccgtaggcc tcgtgcatgt cgattctggc ggtcccatcc
     2701 gagctcactt ccagcgtcct gcggtactgc ccgccgggtt tctgctgcat cccaccgatc
     2761 cgcggtacgc tgtgaccgta agacgaggca aagacgtttt cgtagcgccg gcgtccgaag
     2821 taggcggcat tgtagaggcc cgcgcccggg tcgcagagaa acgtcgtttc gccgatacgg
     2881 agcacaaaac tgcccacgtc gttgtggttg tgctgttcgg cattgttgcc ggccttggcg
     2941 acgagaatca ccgaccggtc accggccttt cccacgcatc gggcgacgcc ggctttgggt
     3001 aacaccacgt tctcgaaaag gggctcccgt cttggctgat cgccgtgcca gatcagattg
     3061 cacaacaccc aacccaggcg gccttcgtgt gccttatcgc ttgcctgggc gagcaatgcg
     3121 gggcgtccgg ttctgtccgc cagcctggcc gcaatgaagg gaagcacact ggcgttctca
     3181 gagctgtccg agaacgaggc gaaagcatgc cgtccgatcg caacggccgt ggggtatccc
     3241 gcgatccttg cgatcttctc gcctgccaga aggtcgatcg ccccgccggt gcgggcccgg
     3301 agcatctcgg cgaagatcac aaagtgcgcc aggccatagt tccagtatcc aatgccctcg
     3361 gtgctggccc cgtcttcctc aaaggcccga tcgacaaagc gattgagctg atccaccacc
     3421 aaggccaatc cgcgggcctg tcgctcggga tccggctcca aaatgagaag aacctgtccg
     3481 atcgagccgg cacagacacc cgtccagttg ttagctccgt tgtcccacca gaacttcgcg
     3541 ccgtggtcaa gatatggttc gatcacacgc ctcttcacct cttcccgaag ccgtgttcgg
     3601 atctcatccg ggagtctggg accgaccagt agctcggtaa acgcgagggt ggcggccgtc
     3661 tccgcggcca tcaggtcgat ctcgccgacg gactcgtgtg cggggatgac ccagttggtc
     3721 tcttcgcaga tgttccacac gagatcgttg acaaagtcta ccgaagcaag atcgccctga
     3781 agccacgcgg ccagaaccgc cttggccaac aacgcccgct tctcgaaata gggcttctgg
     3841 tagggctccc ggtcgccgga tttcctgaat tcccgatact gcgtgtaggt tgtaacggga
     3901 atctccgccg aaccggactt ggccgcctcc agaatcgctt cggcgatcga tgtcctgccg
     3961 agatcaagag gacaagcctt cgacaaccca gggagaacct cggcggcatc ggccggcaca
     4021 cggccaagga ggccgctcat tgtttccacg gcctgagtcg ttggcacatt ctggccgagt
     4081 gaccaggaag gcacgaaaag gatccaggtg ctgatcgcag ctatcgacat ggtgcaggtc
     4141 ttcttcatga attccggcat cctctccgtc cgtcgccccc ccatttctca atctccgact
     4201 ctgaattcct cggcattgcg tcacgcaacg ctgacgcctg tggccgtgcg atgtcatcga
     4261 tggtcgcgtg cataatcagg cagcgaggcg ggccgtaaga gtcctttccg ttatgagcgc
     4321 tgttcactgc ggtatagaac aggtgatacc ggccgccgcg ccgcacgagg ccgcttgggg
     4381 catagatgcc atagctgtcc ggctgagtct ggtcgccacg gggaatccag gccttgcgag
     4441 ccgggcgatc ccagcgcacg ccgtcctcgg aatagcacca ctcaaggtcc atggtctggg
     4501 cttgacaacg gtagtgtccg agcattccga ttcgtccctt gggcgtgtgg gtcacgctga
     4561 gataataaaa ctgctgatcc ggcgggtctt tggcatcgcg gtcgatcacc cgccgccgct
     4621 tctcgaaatg aagaccgtcg tcggacacat accgatcgac caagcggata agccctggag
     4681 cattgtcgtg cggcatgtag gccgggtcat ccttgggaac ctggatcagg gcgacggagt
     4741 acatttcgaa ggtgccgtca ggcaattggt agatgttggt cgcatcgttg gagagcagat
     4801 gcgagggggc caacggctcg tcggctggtc ggtccctgct cggctccttg tgcagcatca
     4861 cgccgtcagg cgaggccacg ccccaggcgg cccggtcgtg gaagtggtaa agcaccggcc
     4921 gaagcggatc gatgacgcgg tagcgatgcc cgtcttcact gtcggccgcg agatagcgga
     4981 gaatacccgg cccgtgggcc cagaagtaga tccgatgttt gccgttcttg agcgggatgt
     5041 gaaccacctg cacggggcgc cacttctccg gcagcggtcc cagcgtgaac gctccatcgc
     5101 cgggctcgcc ggttgaacac tgcaccgggt atttcttcat ctcctgtccc ggcacaccct
     5161 cggcgtaggc aatggtgtat ttcacagtct catgcgtgcc caccgccgag taccagattc
     5221 gccatcggtc cccatcgagc ggttcggagg gcgaggtgaa gctttgaatg ccattctgct
     5281 cccacggttc ggtggcatcc agcacgatgc cgtgtttgtg ccacccgttc agattcgcgg
     5341 gagcggaggc aaccggctcg gcagtccgtg cctggcgcac gccagtcgcg gccaacgtgc
     5401 ctgcaagggc gcccagaccc cgtttgagta tcgtcctgcg gtcgatatcg cgggccatga
     5461 tcaacctcct cgcctggtga gttatttgtg ccggccacag catcagcttt gcacagatac
     5521 gtcggaagat caagcaacac agcccgacgc tgtgcccatt ttccctggca gccccgcccg
     5581 tgggtcgata ccatggcctg tgtggtcccg taggatcccg ggccgccgca ttgggagacc
     5641 tcgatcatga caaggacacc cgtcttttgg ggctttctgt cagtctccat gtcggcgatg
     5701 acggtcgcca tcgccaatcc agtcagcgac gaagaaacaa cacccggcaa gtacctggaa
     5761 gcggtgcaga ccttcgcgga caacgtcctt cgccatggca aggacgtgta tggttccaag
     5821 cacacgccct tgttcgccga cgggctcaac atcgataccc atgagccggt gatctggaag
     5881 ttgcccaagg agcaggccga aaaatggagg atgccgacgg agtggacgct gtcgaatttg
     5941 gccagccaac agaacctgtt tcgagtgttc gtggccctga cggacctgac gggagaccag
     6001 aagtacaagc gggcggctgt cggcgccact cgatatgcgc tggatcatct ccgccacgag
     6061 aacggcttgc ttttctgggg c
//
LOCUS       DAOTPW010000299         6045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11194_length_6045_cov_5.157095, whole genome shotgun sequence.
ACCESSION   DAOTPW010000299 DAOTPW010000000
VERSION     DAOTPW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11194_length_6045_cov_5.157095"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1737
                     /gene="ahcY"
                     /locus_tag="P5316_19485"
     CDS             313..1737
                     /gene="ahcY"
                     /locus_tag="P5316_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRR87198.1"
                     /translation="MATAVTDFKVKDLSLADWGRKEIAIAESEMPGLMAVREEYAGRK
                     PLAGAKITGSLHMTIQTAVLIETLVELGAEVRWASCNIFSTQDHAAAAVAKRGIPVFA
                     YKGESLEEYWEFTLKALSWPDGDGPTSIVDDGGDATLLIHRGRAFEAEFAKTGKHPAI
                     CKDNHEIEVVDNLLRRVREKDARFFHKMAGRVIGVTEETTTGVHRLYEMSRNGELLFT
                     AINVNDSVTKSKFDNLYGCRESLMDGIKRATDVMIAGKKCVVCGYGDVGKGCAQAFRG
                     LGATVYITEIDPICALQAAMEGYQVVTMDDACKWADIFVTATGNVDVITREHMDQMKD
                     QAIVCNIGHFDSEIQVNRLGDLKWEEIKPQVDHVIWPDGKRIIVLAKGRLVNLGCATG
                     HPSFVMSNSFTNQVIAQIELWENRNTGKYEKKVYTLPKHLDERVATLHLSKLGAKLTK
                     LTEKQARYIGVPVNGPFKPEHYRY"
     gene            complement(1905..3995)
                     /locus_tag="P5316_19490"
     CDS             complement(1905..3995)
                     /locus_tag="P5316_19490"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HRR87199.1"
                     /translation="MKSLRLAACVSLVLGGSMTGLAAEASLRTQSSPVGELATAMLGP
                     GWTGPLGEKDLLWRLALRGPDSATAELTSASVPWIEAEGAGSTAYTFVWLGELKNGEV
                     RVTLTITEGRRATGWRLRAGLPDGWKVVRADWPILPNIRTEPGLRMAAPFGWGLEYDV
                     KPGMSYEGAYPSLVTSMPFVAFYRDGKGLYVGVHDRKGHHKHLAVKASEDRVCVTCTH
                     WPSDADTVGGRYAPEFEAVVSAFDGDYWNGAQIYRAFTRETPWGGDKGGLRLENRNQP
                     VPEWLKDIDLWLMPGPEPLKNVEACRRAGEFFGVPIGLHWYNWHEIPFDTLYPEYFPA
                     KPHFREGVKALQEAGFRVMPYINGRLCDPNSKTWTQEGGEQAAARQEDGKPYTEEYGS
                     KVPLNVMCPYTVFWQDKIVGIVDRLVNGYGVDGVYIDQIGAAHAVRCFNPDHGHSRGG
                     GTFWFDGYRKMLDQVRARLPKDRMITTEENAECWNDQFDALLMVNTPSTGGRRIIPLM
                     PAVYGGRIITFGFQYMAGDDIVKSLPFRAKMARAFLWGAQLGWIGVDSIMADNARKEA
                     EFLRNLAVARRGGHEFLLTGTFLGEAEVTGDNPRLIGEGTGRGSPYAIDLPAVMATAW
                     LATDGTVGLAVVNLSDRPRTVEIRPPWNRLNELETGAAPKPAADDQPQAAPITIAASD
                     ARVLRAAGTTRPSR"
     gene            4235..5809
                     /locus_tag="P5316_19495"
     CDS             4235..5809
                     /locus_tag="P5316_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87200.1"
                     /translation="MSMHEKLSGCACCRMSRRRFLATGCATCVGATGILTAGSTARAA
                     EGPSKIRVRIIYSLHGPKQDRPDWPNKGFDFQPVMDRITNTLKEACPRFEFLTSMADG
                     EEAANKILEQDKTADIDGYLVYQMNAWNRVVQTIAASGKPTLYADFQYGGSGGFLVYG
                     AKFLRDKTPNVGFVASSRMEDVVAAVRCFRVIKRGGSMADFVAATANARRQRTPKPGD
                     LTTKPDKVEALTPKDCLARMKESKILTVEKGWHNMDEAVKKEMGIDVVQIPFKELNDA
                     WEAADKNESKAIADRWQKTAAKIEGVSRETLEQSAAMYLAEKALLRKHGANAITINCL
                     GGFYGNHIHAYPCLGFHELLNEGLIGACECDLRSTCTMVAMTALTQGRPGFISDPVID
                     TSSRQIIYAHCVASNKPFGPKGQANPFEILTHSEDRQGASLRSIMPLGYMTTTVEFRP
                     ELKVVLFHQGKAVANVDDDRACRTKLAVEPVGDIEKLFTEWDQWGWHRVTFYGDLKEP
                     VFALADTLGWKVLQEA"
BASE COUNT         1244 a         1979 c         1786 g         1036 t
ORIGIN      
        1 atcggtgtgc tgagttctac gaacgaatgg gtgaccgctg gtggggtttc gagcctgccc
       61 aagtgtccga ttggctcgca caagcgggat ttcgagaggt tcgagccgcg ccgctgcgca
      121 cggccagacc aagtgaccgc agccgagtgg atgcacccgg attgtttgtc gtgaccgggc
      181 gggtttcagc gccttgatcg cgcgagcggt gaaacacgga acgtaagccg agccttccgc
      241 gaagcgcaga atggctttgg caagtcaaaa acagtgcaaa gacgcaagga tttctgaaca
      301 ggagcataac aaatggcaac cgccgttacg gatttcaagg tcaaggatct gtcacttgcc
      361 gactggggtc gcaaggaaat cgccatcgcc gagagtgaaa tgcccggctt gatggccgta
      421 cgtgaggaat acgcgggtcg caaaccgctc gccggggcca agatcaccgg ctcgctgcac
      481 atgaccattc agacggccgt gctgatcgaa acgctggtcg aactgggagc cgaggtccgc
      541 tgggcaagct gcaacatctt ctcgactcag gaccacgccg cggcggccgt tgccaagcgg
      601 ggaatcccag tcttcgcgta caaaggcgaa agcctcgagg aatactggga attcacgctc
      661 aaggcgctga gctggccgga cggcgacggg ccgaccagca tcgtcgatga cggcggcgat
      721 gccacgctgc tgatccatcg cggccgggct tttgaggccg agttcgccaa gaccggcaag
      781 catccggcca tctgcaaaga caaccacgag atcgaagtcg tcgacaacct cctgcgccgc
      841 gtccgcgaaa aagacgcccg gttcttccac aagatggccg ggcgtgtcat cggcgtcacc
      901 gaggaaacca ccacgggcgt ccatcgcctt tacgagatgt cccgcaacgg cgaactgctc
      961 ttcaccgcga tcaacgtcaa tgattcggta accaagagca agttcgacaa cctctacgga
     1021 tgccgcgaga gtctgatgga cggcatcaag cgggccaccg atgtgatgat cgccggcaag
     1081 aagtgcgtgg tctgcggtta tggcgacgtc ggcaagggtt gtgcccaggc gttccgcggg
     1141 ctgggcgcca cggtctacat caccgaaatc gacccgatct gcgcattgca ggccgccatg
     1201 gaaggctacc aggtggtcac catggatgac gcctgcaaat gggccgacat cttcgtcacc
     1261 gccaccggca acgttgacgt catcacccgt gagcacatgg accagatgaa agaccaggcc
     1321 attgtctgca acatcgggca cttcgatagc gagatccagg tgaaccggct cggcgatctc
     1381 aagtgggagg agatcaaacc gcaggtggac cacgtcatct ggcccgatgg caagcggatc
     1441 atcgtgctgg ccaagggccg cctggtcaac ctcggctgcg ccaccgggca tcccagcttc
     1501 gtgatgagca acagcttcac caaccaggtg atcgcccaga tcgaactgtg ggaaaaccgc
     1561 aacaccggca agtacgagaa gaaggtctac acgctgccca aacacctgga cgagcgcgtc
     1621 gcgaccctgc acctgagcaa actcggggcc aagctaacca agctgaccga gaaacaggcc
     1681 aggtacatcg gcgtgccggt caacgggccg ttcaagccgg agcattaccg atactgatgg
     1741 gttcccgacc caaggagcag gacgccgaat cgacatctgg agcagccgtc gccacagcca
     1801 cacaccggtt gcccagaaga ctccctcccc tgccgcgggt gtcccaaggc gctcgtgatc
     1861 cccatgcacg gagatgtctt gaccccctgc ttcctgtaga tcccctaccg tgatgggcga
     1921 gtcgttcctg ccgcacgcaa aacccgggcg tcggaggccg cgatggtgat cggtgccgcc
     1981 tgaggctggt cgtcggcagc gggcttcgga gctgcgccgg tctccagctc gttcagacgg
     2041 ttccagggtg gccggatttc cacggttcgc ggccggtctg aaaggttcac aacggccagg
     2101 cccacggtgc cgtccgtggc cagccaggct gttgccatca cggccggcag gtcgattgcg
     2161 tacgggctgc cgcgaccggt gccttcgccg atgagccggg gattgtctcc cgtgacctct
     2221 gcctcgccaa ggaacgtgcc agtgagaagg aactcgtgtc ctcctcgtcg ggcgacggcc
     2281 aggtttctca ggaattcagc ctccttgcgg gcgttgtcag ccatgatcga atcgacgccg
     2341 atccaaccca gttgcgcgcc ccacaggaac gcgcgagcca tcttcgcacg aaacggcagc
     2401 gacttgacga tgtcatcccc ggccatgtac tgaaaaccga aggtgatgat gcgtccgcca
     2461 tagaccgccg gcatcagagg gatgatccgc ctcccgccgg tcgatggcgt gttgaccatc
     2521 agcagagcat caaactggtc gttccagcat tcggcgttct cctcggtcgt gatcatacgg
     2581 tccttgggca accgcgctcg gacctgatcg agcatcttgc ggtagccgtc aaaccagaaa
     2641 gtccccccgc cgcgcgaatg accatggtca gggttgaaac atcgtaccgc atgggccgcg
     2701 ccgatctggt cgatatacac gccatcaaca ccatatccat tgaccagccg atccacgatg
     2761 ccgacgatct tgtcttgcca gaaaacggta tacgggcaca tgacgttgag cggcaccttc
     2821 gagccgtatt cctcggtgta cggcttgcca tcctcttgcc gggcagcggc ctgctcgccg
     2881 ccttcctgcg tccaggtctt cgagttcgga tcacacaagc gaccgttgat gtacggcata
     2941 acacggaacc cggcctcctg cagagccttc acaccctcgc gaaagtgcgg cttggcaggg
     3001 aagtactcag ggtaaagcgt gtcgaacggg atctcgtgcc agttgtacca gtgtaggccg
     3061 atcggcacgc caaaaaactc gcccgctctg cggcaggcct cgacgttctt aagcggctcc
     3121 ggaccgggca tgagccagag gtcgatatcc ttgagccact ctggcacagg ctgattccgg
     3181 ttctcaagcc gtagcccacc tttgtcccct ccccatggcg tctcgcgagt gaatgcccga
     3241 tagatctgag cgccgttcca gtagtccccg tcgaatgcgc tgacgaccgc ttcaaactcc
     3301 ggtgcataac gacctccaac cgtatccgca tccgaaggcc agtgcgtgca ggtcacgcag
     3361 acacggtctt cgctcgcctt gacggccaga tgcttgtgat ggcccttcct gtcgtgaacg
     3421 ccgacataca gccccttgcc gtcgcgatag aaagctacga agggcatcga ggtcaccaga
     3481 gatgggtaag cgccctcata gctcatgccc ggcttcacgt cgtattccag cccccagccg
     3541 aacggagctg ccattctcag gccgggctct gtccggatgt tcggcaggat cggccaatcc
     3601 gcccgcacca ccttccaacc gtcgggcaaa ccggctctga gccgccaccc cgtcgcgcgc
     3661 cgaccctccg tgatggtcaa agtaacccgc acctctccat tcttcagctc gcccaaccag
     3721 acaaacgtgt atgccgtgct gcccgccccc tctgcctcga tccatggcac gctcgcgctg
     3781 gtcagttctg ccgtcgccga gtccggcccc cgcagagcca gtcgccacag aaggtccttc
     3841 tcgcccaacg gtccggtcca tcccggcccc agcatcgcgg tggccaactc gcctacaggc
     3901 gacgactggg tgcgaagaga tgcttcggcc gccaagccgg tcatcgaccc gccaagaacc
     3961 aatgatacgc atgcagccaa acgcaggctt ttcatatcga tcacctcgca tcagtcggcc
     4021 gagcccggat agacacctgt gagacttcga aacgccgggc agtgtaccac ccctgaggcg
     4081 cgctttgtat ctggccgctg accagccgtt cgggcgcgcc gtttgacgct ggcaccaacc
     4141 tttcgatagc atggcggtct ggaggagccc cgggcgcggc gtcgcccggg cactcgcagc
     4201 aagtccagtc cactcgataa cggaggatcc agtcatgagt atgcatgaga agctttcggg
     4261 gtgtgcgtgc tgtcggatga gccgccggcg gttcctcgcg accgggtgcg caacatgtgt
     4321 cggggcaacg ggcatactga cggccgggtc caccgcgcgg gctgccgaag ggcccagcaa
     4381 gatccgcgtc cgcatcatct actcactgca cgggcccaaa caggaccggc ccgactggcc
     4441 caacaaaggc tttgacttcc agccggtgat ggaccggatc accaacacgc tcaaggaggc
     4501 ctgcccgcga ttcgagttcc tcacctcgat ggccgacggc gaggaagccg ccaacaagat
     4561 cctggagcag gacaagaccg ctgatatcga cggctacctc gtctaccaga tgaacgcctg
     4621 gaaccgcgtg gtgcagacca tcgctgcttc cggcaaaccc acgctgtacg ccgacttcca
     4681 atatggtggc agcggcgggt tccttgtcta tggcgccaag ttcctgcgcg acaagacgcc
     4741 gaacgtcggc ttcgtcgcat cctcgcggat ggaagacgtg gtcgcggccg tccgctgctt
     4801 cagggtgatc aagcggggcg gctcaatggc cgacttcgtc gcggctacgg ccaacgcccg
     4861 ccgacaacgc acgcccaagc ccggcgatct caccaccaaa cccgacaagg tcgaagccct
     4921 cacgcccaag gactgcctcg cccgtatgaa ggaatcaaag atcctcaccg tggaaaaggg
     4981 ctggcacaac atggacgagg cggtcaaaaa ggagatgggc atcgacgtcg ttcagatccc
     5041 cttcaaggag ctcaacgacg cctgggaggc cgcggacaag aacgagtcga aggccatcgc
     5101 cgaccgctgg cagaagacgg ccgcaaagat cgagggcgtc tcccgcgaga cgctcgaaca
     5161 gtccgcggcg atgtacctcg ccgagaaggc cctcctcagg aaacacgggg ccaacgccat
     5221 caccatcaat tgcctcggcg gcttctacgg caaccacatc cacgcctacc cctgcctggg
     5281 gttccatgaa ttgctcaacg aagggctgat cggggcttgc gaatgcgacc tccgctcgac
     5341 ctgcactatg gtggcaatga ccgccctgac gcagggacgg ccgggcttca tctccgaccc
     5401 ggtcatcgat acttcgtcgc gacagatcat ctacgcccac tgcgtggcct cgaacaaacc
     5461 gttcgggccc aagggccaag ccaacccatt cgagattctc acccactccg aggaccgcca
     5521 gggcgcctca ctccgctcga tcatgccgct gggctacatg accaccacgg tcgagttcag
     5581 gccggaactc aaggtcgttc tcttccacca gggcaaggcg gtcgccaacg tcgatgacga
     5641 ccgggcctgt cggaccaagc tggccgtcga accggtcggc gacatcgaaa aactctttac
     5701 cgagtgggac cagtggggtt ggcaccgcgt caccttctac ggcgacctca aggagccggt
     5761 cttcgccttg gccgacaccc tcggctggaa ggtgttgcag gaagcgtgag cccatctttc
     5821 agaaccgcga ccgtaaggga gcggcaggaa agcattatca gtcgtcatca tggtgcggct
     5881 catcctgccg atcaatgcgg ttcatcctgc cgatcaacca cgtagttgca ggccgcggcg
     5941 agctgttcct ccgtccatag gggtgtcgaa acgggtacgg agctagcacc acagcgcaag
     6001 gtggttgatt tgaaccaagc tcaggcctcg ttacgccccc tggcg
//
LOCUS       DAOTPW010000300         5989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11294_length_5989_cov_5.411358, whole genome shotgun sequence.
ACCESSION   DAOTPW010000300 DAOTPW010000000
VERSION     DAOTPW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11294_length_5989_cov_5.411358"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1061
                     /locus_tag="P5316_19500"
     CDS             147..1061
                     /locus_tag="P5316_19500"
                     /inference="COORDINATES: protein motif:HMM:NF021628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HRR87201.1"
                     /translation="MAVINTGLLTKGLRSEFFDRMGKVTTYYQDLSTRIPSTSDSETY
                     RWLGTVPRMREWGTGRLAKGLRSESYSVENLKYEATIEVDRDEIADDQTGQIRIRVGE
                     LAQRAATHKDYLLAQLLINGGTAGFNSYDGVPFFSHSHVSGQSGTQDNDIAYDTSLPN
                     DPTSEEMKGALKRAIGRLLSFKDDQGEPMALSPEGLVCVVPPSMLYTALETVSAAMIG
                     QTTNVLSGVARVISLPGLSAADTWYLLKTDGVVRPFIFQDREPIEFGALAEESEEGFK
                     REKWLFGVRARYRVIYGYWQFAVRVVFN"
     gene            1156..1599
                     /locus_tag="P5316_19505"
     CDS             1156..1599
                     /locus_tag="P5316_19505"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HRR87202.1"
                     /translation="MSYATNADIEERLGTATYIELTDDTGSGQPDEDKVTEARLAAEA
                     EIDSYLGRRFAVPIDVGGQPGLVAMLKRLTLDLAEYRLRMRRPPVTKDARLQCDAALL
                     WLSRAANAEVALPTIVELPSNSGLGPAAETVGGERVLTGDGWEAV"
     gene            complement(1640..2350)
                     /locus_tag="P5316_19510"
     CDS             complement(1640..2350)
                     /locus_tag="P5316_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87203.1"
                     /translation="MTTVNKVCILLGLAPTVIVLPGCRSPGSLRPSEYLVVDLLDEFG
                     LDQPSDSWELATPDAWRIGSDGQRRFLQVAALSASPDKQPPAWESAMHRKYRFRNFSL
                     SAWLRLEQAVGNRPCSASIVFGWRNDLNCLRLDLSDLSGKADVALVQVTDGQVIRLAA
                     AALSSRPDFTRKEWHQIGILRNLDTATVEVYIDEADKPVLRARTTAHEWGRLGLASTT
                     GGASFGRVMISGEALPAQ"
     gene            2618..3007
                     /locus_tag="P5316_19515"
     CDS             2618..3007
                     /locus_tag="P5316_19515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR87204.1"
                     /translation="MAAPYDGKSILVVDDDPDILTAIVELFKESGAEITTASDGNTAV
                     NLAMTKKPDLLILDAMLPRRSGFLVLEKLKARKPPGTKPFVIMITGNQGKRYQECAET
                     FGAEDFINKPFRMDRLRQSVEDLLAKA"
     gene            3133..4416
                     /locus_tag="P5316_19520"
     CDS             3133..4416
                     /locus_tag="P5316_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR87205.1"
                     /translation="MERSKVAILRTRPATVLADYHELMNLAGYRDVIDPSADTALKVN
                     ISWHFFYPGSSTTPWQLEGVIRAMKRDGYDPRLMHACHNRTVVIDAHLGERENKQLNV
                     VRAHGLRNVHLYEGEEWINIRDAVGDLTKSFLCLNEVYPKGFTIPRRFLGENILHLPT
                     IKTHVFTTTTGAMKNAFGGLLNEHRHWTHPVIHETLVDLLMIQKKIHRGVFAVMDGTF
                     AGDGPGPRCMVPYVKNVLLASADQVAIDAVAARLMGFDPMQDCRFIRLAHELGLGCGD
                     PSQIEIVGDTEAARENWHFEGPFKKMTFASSMQHKIYWGGLKNALEWSLKTWMAPWAY
                     IASVLYHDMYWYPYNGKSRVEEILRSEWGRLFHNWEKVAPDENGWPDVGIEPAKLDRS
                     TLQLIGKAFRILGTCVSEAPETRRLRGRKIVTATR"
     gene            4918..5109
                     /locus_tag="P5316_19525"
     CDS             4918..5109
                     /locus_tag="P5316_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87206.1"
                     /translation="MEKPKRSYYLPGKLVTAFDKECSKAGYVKEKVVAAAMFEFLNSS
                     PDQRAAMFDRLDKFLSKKG"
     gene            5111..>5989
                     /locus_tag="P5316_19530"
     CDS             5111..>5989
                     /locus_tag="P5316_19530"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRR87207.1"
                     /translation="MKTWQVAALFFVLAGALRAEFAIHDGDTVVFLGDSITAARGYGK
                     IVENYTLLRYPDRRVRFINAGWGGDTAAGGAARLERDVLGHGATLVTVAYGVNDIGWG
                     GKADDEHKQKYLDGIRDIVTQCRSRGVRVFICSAAITGADPDKSENDYLQRMCDEGMA
                     LSRSLGGGAIDVQRSMREIQKRIWAANHEAKDDKDKHTLHAADGIHLNDLGQLAMGFA
                     ILKGLGAPAEVSSVTIDAAAGRVAEASGCKVSELKVTTETVEFDRLDEGLPFTLGPIG
                     ALQFRFIPIPDQLNRYM"
BASE COUNT         1256 a         1709 c         1922 g         1102 t
ORIGIN      
        1 ggtgccagca acgccgagat ggatgccatt tacgactcca tcacattggt gtgcattgga
       61 gccaaagcat ggtcggtaac ggcgaagcgc attgcgtaag gatcaaatgg cctcaagggt
      121 gtttgtgaaa cggaaaggaa gcagccatgg cggtgattaa caccggattg ttgaccaagg
      181 gactccgcag cgagttcttt gatcggatgg ggaaggtaac gacctattac caggatctgt
      241 cgacacgaat tccgtcgacg tcggattcag agacataccg ctggctcggc acggtgccgc
      301 ggatgcgaga gtggggcacc ggccggctgg cgaagggatt gcggagcgag agctactcgg
      361 tcgagaacct caaatatgag gcgaccatcg aagtggatcg cgatgagatc gcagacgacc
      421 agaccggtca gatccggatc cgggtcggcg agctggccca gcgggcggcc acgcacaagg
      481 actacctcct tgcgcagctt ctgatcaacg gaggcacggc cggtttcaac agctacgatg
      541 gggtgccctt tttcagccat tcgcacgtat cgggacagtc agggacgcaa gacaatgaca
      601 tcgcatacga cacctcgttg ccaaacgacc cgacaagcga ggaaatgaaa ggcgccctca
      661 agcgggcgat cggccggctc ctgtcgttta aggacgatca gggagaaccg atggcgctga
      721 gccccgaggg ccttgtctgt gtcgttccgc cttccatgct gtacacggcc ctcgagacgg
      781 tgagcgcggc gatgatcgga cagactacga atgttctgtc gggggtcgcc agagtaatct
      841 cgctgccggg cctttcggca gcagacacct ggtacctgct caagaccgac ggcgtggtgc
      901 ggcccttcat cttccaggat cgcgagccga tcgagttcgg tgctctggcg gaggaaagcg
      961 aagagggctt caagcgagag aagtggctct tcggtgtgcg ggcgcggtat cgagtgatct
     1021 acgggtactg gcagttcgcg gtgcgggttg tcttcaactg accagaggga cgtggagcga
     1081 ggggcaaggg cggcgatgcg ggcttggggc gctgaacttg cctccgctcc cggccagtga
     1141 tgacgggaga gtctcatgtc ttacgcgacc aacgcggaca tcgaagaacg tctgggtacg
     1201 gcgacctata tcgagctgac ggacgacacc ggctcaggac agccggacga ggacaaggtt
     1261 actgaagcaa ggctcgcggc cgaggcggaa atcgacagct atctgggccg gcgtttcgcg
     1321 gtgccgatcg acgtcggcgg gcagccggga ctggtagcaa tgctgaagcg gctgaccctg
     1381 gatctagccg aatatcggct gcgaatgcga cgaccgccgg tgacgaagga cgcccggctg
     1441 caatgcgacg cggcactgtt gtggctcagt cgagcggcta atgcagaagt ggcgttaccg
     1501 acgatcgtcg agttgccgtc aaacagcgga ctcggcccgg cggccgaaac ggtgggcggc
     1561 gagcgagtgc tgacgggcga cggatgggaa gccgtctgac cccacgggac ggtagggcaa
     1621 tcggggattc ctcgtgtgac tattgggcgg gcagcgcctc cccgctgatc atgacccgtc
     1681 cgaacgacgc gccgccggtc gttgaggcaa gtcccaaccg accccattca tgcgcggtcg
     1741 tcctggctcg cagcacaggc ttgtcggcct cgtcgatata gacctccacc gtcgcagtat
     1801 cgaggtttcg gagaatgccg atctggtgcc attcctttcg cgtgaagtcg ggcctggaag
     1861 acaaggccgc ggcagccagc cggatgacct gaccgtcggt tacctggacc agtgcgacgt
     1921 ctgctttgcc ggagaggtcc gataggtcca atcgaaggca attcaggtca ttccgccagc
     1981 cgaagacgat ggaggcgctg cacggacggt tgccgacggc ttgctcgagc ctcagccagg
     2041 cggagaggct gaagttgcgg aacctgtatt tgcggtgcat tgcggattcc caagcaggcg
     2101 gctgcttgtc gggactggct gacaaggcgg caacctgaag gaatcgtctt tgcccgtccg
     2161 aaccgattcg ccaggcatcg ggtgtggcca attcccagga atcgctgggc tggtcgaggc
     2221 cgaactcatc caggaggtca acgaccagat actcgcttgg ccgaagggac ccaggagagc
     2281 ggcaccccgg caggacaatc acggtgggtg ccagcccgag cagaatgcag actttgttaa
     2341 cggtggtcat gcttggatct ccggggccgt cttgcaggca cacctcagct tatcggtaca
     2401 ggatactttc ccgttacgca acccgcgacg agcagacagc cttccgtcct tcccccctgt
     2461 gcggtttcag caggggcttt ttccctaaag cgtgccaaag ccgggcggat tgttgcatgt
     2521 gcgagcggaa gcggatatac tctcatacaa gagccacgca tgtatcacac gcagtacgac
     2581 atgctacact ggaattgcct ccggaggatt atgacatatg gcagcaccgt atgatggaaa
     2641 gtccattctg gttgtggacg acgatcccga tatactgacc gcgatcgtcg agctgttcaa
     2701 ggaaagcggg gccgaaatca ccacggcttc cgacggcaat acggccgtca acctggcgat
     2761 gaccaagaag cccgatctct tgatcctcga tgccatgctt ccgcggcgga gcggattcct
     2821 ggttctggag aagctcaagg cccgcaaacc gccggggacc aagccgttcg tgatcatgat
     2881 taccggcaac cagggcaagc gctaccagga gtgcgccgag accttcggcg cggaagactt
     2941 catcaacaaa ccgttccgga tggatcggct gcgccagtcg gtcgaggatc tgttggccaa
     3001 agcctgagcg atcgacccat ccgtcctgtt gaacggtcct catcgtcaac ccaggcgtgt
     3061 tcgggaccag ccgcgcacgc cttatctttg cgcggcgttg ctgaaagccg tcaaggagca
     3121 tagaatactc tgatggaacg ctccaaggtg gcgatactgc gcacgcgacc cgcgaccgtc
     3181 ctggctgact atcatgagct catgaacctg gccggatatc gcgatgtcat tgacccgtcc
     3241 gccgacaccg cgttgaaagt caatatctcc tggcatttct tctatcccgg cagcagcacc
     3301 actccctggc agcttgaggg cgttatccgg gcaatgaagc gggacgggta tgacccgaga
     3361 ctgatgcatg cgtgccacaa ccggacggtg gtcatcgatg ctcacctggg ggagcgggaa
     3421 aacaagcaac tcaacgtggt tcgggcccac ggactgcgga atgtacatct ttacgaaggt
     3481 gaggagtgga tcaacatccg cgacgcggtg ggcgacctga cgaagagctt cctgtgcctc
     3541 aacgaggttt accccaaggg gttcacgatt cctcgtcgat tcctcggcga gaatatcctg
     3601 cacctgccga cgatcaagac gcacgtcttc acgaccacga ccggtgcgat gaagaacgcc
     3661 tttggcggcc tgttgaacga acaccgacac tggacgcacc cggtgatcca cgagacgctc
     3721 gtggacctgc tgatgatcca gaagaagatc caccgcggcg tctttgccgt gatggacggg
     3781 accttcgccg gtgatgggcc tgggcctcgc tgcatggtgc cctacgtcaa gaacgtgctt
     3841 ctggcctcgg ccgaccaggt cgcaatcgac gcggtagccg ccagactgat gggatttgac
     3901 ccgatgcagg actgcaggtt catccggttg gcccacgagt tggggctggg atgcggcgat
     3961 ccctcgcaaa tcgagatcgt cggcgacacg gaggccgccc gggaaaactg gcacttcgaa
     4021 ggcccgttca agaagatgac gttcgccagt tccatgcagc acaagatcta ctggggcggg
     4081 ctcaagaacg cgctggagtg gtcactcaag acgtggatgg ctccgtgggc atacattgcg
     4141 agtgtgcttt accatgacat gtactggtac ccctacaacg ggaaaagccg ggtcgaggag
     4201 attcttcgaa gcgaatgggg ccggctgttc cacaattggg aaaaggtggc gcccgacgag
     4261 aacggctggc cggacgtcgg gatcgagccg gccaagctcg accgaagtac gctgcaactg
     4321 atcggcaagg catttcgcat ccttggcaca tgcgtttccg aggccccgga aacccgtcgg
     4381 ctgcgcgggc ggaagatagt caccgcgaca cgctgacatt gcgtcgcacg gataccggac
     4441 aaggcctcgt aggcagaggc gtttcagttc tctcgaagag cggccaacag atggtatggc
     4501 tgttgtgaag gcaccctgct aatggggcct gcgaaggggg tttgtctcaa gcacggaaag
     4561 acctgggatc atctgaccag agaacctgcg ggcaagccgc cggtggtccc catggcagac
     4621 ccccgccgta tctgtaacgg cccccgaact gatcggagca ggttacaggg ccgcgccggg
     4681 aatctggcgc tcctgccgga agatggcggt cgcccccgac gtattcgtag tcccgcctca
     4741 ggtggaagga tcggcatctg ccccctctgc cccactggcc ggagtctatc ggctctggct
     4801 gggcagcaaa acctcttgct ttgcccagag ggagactgta tcatgaagct ccgatcagaa
     4861 gtcctgacgt gtgcgattca agaaaacgac tgatttcaac acagggagat ttcgatcatg
     4921 gaaaaaccca aaagatccta ttatctgccc ggcaaactgg tgacggcttt tgacaaggag
     4981 tgttcgaaag cggggtatgt caaagagaaa gtcgtggcgg cggccatgtt tgagttcttg
     5041 aactccagcc cggatcaacg agccgcgatg tttgacaggc tcgacaagtt cctcagtaag
     5101 aaaggttgaa gtgaaaacct ggcaagtcgc ggctcttttc tttgttctcg caggtgctct
     5161 gcgagccgag tttgccatcc atgacggcga tacggtggtg tttctgggcg acagtatcac
     5221 cgccgcccga ggctacggta agatcgtcga gaactatacg ctcctgcgtt acccggaccg
     5281 cagagttcgt ttcatcaacg cgggttgggg cggggacacg gccgccggcg gcgctgcccg
     5341 gcttgagcga gacgttctgg gtcacggggc tacgctggtg acggtggcct acggcgtcaa
     5401 tgacatcggc tggggcggca aggccgacga cgaacacaaa cagaagtacc tcgacggtat
     5461 tcgggacatt gtcacgcaat gcagaagtcg cggcgtgcgg gtgttcatct gctcggcggc
     5521 gatcaccggg gctgaccccg ataagagcga aaacgactat ctccagcgga tgtgcgatga
     5581 gggcatggcc ctgtcgcgat ctctcggcgg cggtgcgatc gacgtgcagc gatcgatgcg
     5641 cgagatccag aagcggatct gggccgccaa ccatgaggcg aaggatgaca aagacaagca
     5701 cacgctgcac gccgccgacg gcatccacct caacgatctc ggccagttgg cgatgggatt
     5761 cgcgattctg aagggccttg gggccccggc cgaggtttct tcggtgacga tcgatgcggc
     5821 cgcggggcga gttgccgagg cctctggctg caaggtttcg gagctgaagg tcaccaccga
     5881 gaccgtggag ttcgaccgcc tggatgaggg actgccgttc acccttggcc cgatcggtgc
     5941 cctgcagttc cggttcatcc caatccccga ccaactcaac cgatacatg
//
LOCUS       DAOTPW010000301         5850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11620_length_5850_cov_5.579465, whole genome shotgun sequence.
ACCESSION   DAOTPW010000301 DAOTPW010000000
VERSION     DAOTPW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11620_length_5850_cov_5.579465"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..540)
                     /locus_tag="P5316_19535"
     CDS             complement(217..540)
                     /locus_tag="P5316_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87208.1"
                     /translation="MRRVRFPTNLEYVAVRGQTPNVQEHATLIAAIRGGKHDNEARNV
                     AESTLSNIMVRQAAYTGKEVLWDELIKSDLELKKPDYELTPENILAHVPIPGGDAFPT
                     KKAKG"
     gene            complement(509..1933)
                     /locus_tag="P5316_19540"
     CDS             complement(509..1933)
                     /locus_tag="P5316_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR87209.1"
                     /translation="MSRKLLSLVAAAAAFLLVSTNDTTGSEVPRTRPSVVFLFADQWR
                     AQAVGYAGDPNAKTPHLDRLAAESVVLTTAVSTCPVCSPYRATLMTGRYPVNNGVFLN
                     DVCLSRDAVSLAQAFAGAGYKTAYIGKWHLDGHGRSSFIPPERRQGFEFWRACECTHN
                     YNHSLFYGDTDEKQFWKGYDADAQTVEAQAYIKAHKNEPFLLVLSWGPPHNPYDTAPE
                     RFRRMFRPEDIKLRPNVPPEAQDAARRDLAGYYAHCAALDECVGRIVETLQESGIADN
                     TILVFTSDHGDMLGSHAQIRKQRPWDESILVPFVVSWPKVLGRSGRKLAAPFNSPDIM
                     PTLLGLAGIAIPATVDGEDRSAWLLGKQPDIDRAALISCVAPFGEWIRGKGGREYRGI
                     RTMRYTYVCSLKGPWLLYDNEEDPYQQRNLVGKPELESLQAKLEGWLRAELSRYKDEF
                     LPGEEYIKRWGYKTDAKGTVPYQP"
     gene            2095..3444
                     /gene="murD"
                     /locus_tag="P5316_19545"
     CDS             2095..3444
                     /gene="murD"
                     /locus_tag="P5316_19545"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRR87210.1"
                     /translation="MRSIDLTDSRVVVMGLGRFGGGIGVTRWLVAQGARVTVTDKESA
                     ESLRHSIEQLSDLPVTYHLGGHLDSDLERCDLLVVSPAVNKAQSEYVRKAVERGIPLS
                     SEMNLFVERCPARRIIGVTGSAGKSTTTAMVGSIASACAQAGRVPAVWMGGNIGHSLL
                     NDLHQMTADDLVVLELSSFQLEDVAMVPWSPSHAVITNIKPNHLDRHVTMEAYTAAKM
                     NIVRFQKPDGLAFVSAADEPLAEEVRKAGAGSRLRRFEFDPAFRKALRVPGRHNQDNA
                     AAAVAVSRSVGIPDDLIARGLSQFTGLPHRLELVGEYNGVQYYNDSKSTTPESALIAL
                     NAFSAPVVMMIGGGDKGMSFDEMCRQIVQKAKGVVCYGATGPKLFDLAGRFAAASTPS
                     ARVEKARSFEEALTVAKAMAEPGDVVVMSPACTSYDMFTNYEQRGERFREIVRSFAT"
     gene            3854..4105
                     /locus_tag="P5316_19550"
     CDS             3854..4105
                     /locus_tag="P5316_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87211.1"
                     /translation="MRWVRKATECRNRPTDWATLGTRIIQTAAVAAAVHMAERLQVVP
                     PTETDLRPRTAKAELDAQPGAPRQEGRKESDAGKEIVED"
     gene            4119..4877
                     /gene="flgF"
                     /locus_tag="P5316_19555"
     CDS             4119..4877
                     /gene="flgF"
                     /locus_tag="P5316_19555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRR87212.1"
                     /translation="MVYGIYQSAAGMIVNQYRQAVLANNLANLDTVGFKHDLTIVRER
                     KVETAEDALSGRLSDRILDRMTGGSLVAPTVTSFRQGEIQVTGRETDVAIEGNGFFAV
                     SDGAKTRYTRDGRFSINNEGLLVTAGGRPVLDDRGKTISVPETARGRIRIGEDGTVRS
                     GDEAFARIGLVDFADRSRLCKVGANMFEAVGTRAEAVSPRLRTNSLESSTVDPTKAMV
                     AMIEVSRAYEINANLVSMADTTLSRAVNDIGRIQ"
     gene            4902..5708
                     /gene="flgG"
                     /locus_tag="P5316_19560"
     CDS             4902..5708
                     /gene="flgG"
                     /locus_tag="P5316_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRR87213.1"
                     /translation="MGITAMYAAATGMKAMDTKLNVVANNLANVGTVGFKRSRTNFED
                     LMYQTLREPGVKDINDEPNPYGILVGLGAAVSGTQLDFRVGSAEQTDKPLDVMIQGEG
                     FFQVTTYYNGEEIRAYTRAGNFFRNANGQLVLGNTDGSVLEPRITLGEDDREIEIDAA
                     GRVSAIQGAGPDRTDIGQIELARFINPEGLKSIGQNLYVETDASGPPITGNPMENGMG
                     SLQNRMLELSNVEPVRELVDLIFTQRGFELNSQSIQGADEMLRVVANLRR"
BASE COUNT         1157 a         1744 c         1858 g         1091 t
ORIGIN      
        1 ccgagaacct ggctaggtcg ggtgcaaagc aggccgccaa aaaggtctat gctgccatcg
       61 cggccggtga cgccgacgcc ccgcagaaaa aggccgccga gatggcccta acggccttgt
      121 gaagcagttg acgctcagcc acacagtagc cctgcgcccc ctgatgcgag gcagaagggc
      181 cgccatccgt tgagcggcgt cttggtcgcc gggcggttac cccttcgctt tcttggtcgg
      241 gaaggcatcg ccgcccggaa tcggcacgtg tgccaggatg ttctccgggg tcaactcgta
      301 atccggcttc ttcagctcga gatcggactt gatcagttca tcccagagaa cctccttgcc
      361 ggtgtaggcc gcctggcgga ccatgatatt cgagagcgtt gattcggcga cgtttctcgc
      421 ttcgttgtcg tgcttgccgc cgcgaatggc cgcgatcaag gtggcgtgtt cctgtacgtt
      481 gggggtttgg cccctgaccg cgacgtactc aaggttggta gggaaccgta cccttcgcat
      541 ccgtcttgta accccaccgc tttatgtatt cctcccctgg aaggaactcg tccttgtatc
      601 gactgagttc tgctctcagc cacccctcca gcttggcctg cagtgactca agttcgggct
      661 tcccgacgag gttgcgctgt tgatacgggt cttcctcatt gtcgtatagc aaccacggcc
      721 ctttaagcga gcatacatag gtgtatcgca tcgtgcggat tccacggtat tcgcgcccgc
      781 cttttccacg aatccactcc ccaaatgggg cgacgcaact gatcagagca gcacggtcta
      841 tgtcgggttg cttgcccagc aaccatgccg agcggtcctc accgtccact gttgccggaa
      901 tcgcgatccc cgccaatccc agaagcgtcg gcatgatgtc cgggctgttg aagggcgccg
      961 ccagctttcg gccggaccgg cccaggacct tcggccagct taccacgaaa ggaaccagaa
     1021 tggattcatc ccaggggcgc tgcttgcgga tctgagcgtg tgacccgagc atgtcgccgt
     1081 ggtcggacgt gaacaccaga atggtgttgt ccgcgatccc gctttcctgc agagtctcga
     1141 caatccggcc cacgcactca tccagcgcgg cgcaatgagc gtagtatccc gccagatctc
     1201 gacgggcggc atcttgtgcc tcgggcggga cattcgggcg cagcttgatg tcctcgggcc
     1261 tgaacatgcg tcggaatcgc tcaggagcgg tgtcgtacgg attgtgcggt ggtccccacg
     1321 agagcaccag caggaacggc tcgttcttgt gagccttgat gtaggcctgg gcctcgacgg
     1381 tttgtgcgtc agcatcgtag cctttccaga actgcttctc gtcggtgtcc ccgtagaaca
     1441 gcgagtggtt atagttgtga gtgcactcgc acgcccgcca gaactcgaag ccctgtcgcc
     1501 gctcgggagg aatgaagctc gatcgcccat gcccgtcgag gtgccacttg ccgatgtagg
     1561 cggtcttgta tccggcaccg gcgaaggcct gtgcgagcga aaccgcgtct cggctgaggc
     1621 atacgtcgtt gaggaagacc ccgttgttga ccgggtaacg gccggtcatc agcgtggctc
     1681 ggtagggcga acacaccggg caggtcgaga cggcggtcgt gagaaccacg ctctcggccg
     1741 ccaggcgatc caggtgaggc gtcttcgcgt tgggatcccc cgcatagccg acggcttggg
     1801 cccgccactg atcggcgaaa aggaagacga cactgggccg cgtcctgggt acttcagatc
     1861 ctgtggtgtc gttcgtcgaa accagaagga acgcggccgc tgcggcgaca agcgacagca
     1921 gcttcctgct catgggtacc tcctggaccg gcattgttgc ctgatgcggc ttcaaaagca
     1981 aaaactccct gcccgacccg gcatgacctt gaacgggatg cttcgcatta tcgccaggca
     2041 acactgccgc tggagcgttt ctcctcgtcc ttggaacgag tacaattcca cgccatgaga
     2101 agcatcgatc tcacggacag tcgcgtggtg gtaatgggcc tgggtcgctt cggcgggggg
     2161 atcggagtca cccgctggct ggtggcccag ggggcgcggg tgacggtaac cgacaaggag
     2221 tcggccgaat cgcttcgcca cagtatcgaa caactgagcg atctgccggt gacgtaccat
     2281 ctgggaggac atctggattc agacctcgaa cgttgtgatc tgctggtggt cagcccggcg
     2341 gtcaacaagg cccaatcgga gtatgtccgc aaggccgtcg aacgcggaat cccgctcagc
     2401 agcgagatga acctgtttgt cgaacgatgc ccggcgaggc gcattattgg tgtcaccggt
     2461 tcggcaggca agagcaccac gacggcgatg gtcggttcga tcgcctcggc ctgtgcccag
     2521 gcgggtcggg tgccggccgt gtggatgggc ggcaacatcg gccattcctt gctgaacgat
     2581 ctgcaccaaa tgaccgccga cgacctggtc gtactcgaac tgtcgagctt tcagctcgaa
     2641 gacgtcgcca tggtgccgtg gagtccttcc cacgccgtca ttacaaatat caagcccaat
     2701 catctggatc gccatgtcac catggaggcc tacacagccg ccaaaatgaa cattgttcgt
     2761 ttccagaaac cggacggcct ggcgttcgtc agcgccgccg atgagccgct ggcggaagag
     2821 gtccgaaagg ccggtgccgg ctcgcgcttg cgacggtttg agttcgatcc tgcgttcagg
     2881 aaagccctgc gagttccggg tcggcataac caggacaacg ccgccgccgc cgtcgccgtg
     2941 tcccgttccg tgggaatacc cgacgacctg atcgcccgcg gcctgtcaca attcaccggt
     3001 ttgccgcacc ggttggagct ggtcggggaa tacaacggcg tgcaatacta caacgattcc
     3061 aagtcgacta cgccggaatc cgccctgatc gccctgaacg ccttctccgc accggtggta
     3121 atgatgatcg gcggtggcga caagggcatg tcatttgatg agatgtgccg ccagatcgtg
     3181 cagaaggcca aaggggtggt ttgctacgga gcgaccggtc ccaagttgtt cgatttggcc
     3241 ggccgattcg ccgcggcctc gacgccctcg gcacgcgtgg aaaaggcccg cagcttcgaa
     3301 gaagccctga cggtcgccaa ggcgatggct gaacccggtg acgtcgtggt gatgtctcct
     3361 gcttgcacca gctacgacat gttcacgaac tacgagcagc ggggcgagcg gttccgcgaa
     3421 atcgtccgat cctttgccac ttgagggcgg cgtcggatgg agattacata actgacgaag
     3481 ttttatgccg tcgaatggaa tagtcgtgta cgtgcccgag cagaccggcg aacaactggt
     3541 ctggtctgag cccgtggatg gcggaacttg accccctcgg cggcaagact tgcgggctgt
     3601 cggcagaagt tgcgcttcct aaaccgatcc ggcctcacca cctggtcgga aacttatcca
     3661 gattaacacc attcataact cttctttgca taatgactta tcccgtcccg tcgaatttcc
     3721 gctccctgag caattcgtat gccgcagtgc tgtggcacgc cctttgacag gcggagggtg
     3781 tgcgtgggtc tgcccgcgca acggcggttc gtggtgccgc cgcccggttt gctgtgggag
     3841 gtgaatcaac cgcgtgcgat gggtgcgtaa ggcaacggag tgccggaaca ggcccacgga
     3901 ttgggccacc ctcggcacgc gaatcatcca gacagcggcc gtggccgccg cggtccacat
     3961 ggccgagcgg cttcaagtcg tgcccccaac tgagaccgat ttgaggccgc gaaccgcaaa
     4021 agcggagctg gacgcacaac cgggggcacc gaggcaagaa gggcggaagg agtccgacgc
     4081 gggcaaagag atcgtcgagg actgacaggg actggttgat ggtatacggc atctatcaat
     4141 ccgcggcagg catgatcgtg aaccagtacc ggcaggcggt gctggccaac aaccttgcca
     4201 acctcgacac cgtcggcttc aagcacgacc tgacgatcgt acgcgaacgg aaagtggaaa
     4261 ccgccgagga tgccctgagc ggtcggctga gtgatcgcat cctcgaccgg atgaccggcg
     4321 ggtcgctcgt cgcaccgacg gtcacttctt tcaggcaagg cgagatccag gttaccggca
     4381 gagagacgga tgtggccatc gaggggaatg ggttttttgc ggtcagcgac ggcgccaaga
     4441 cgcggtatac gcgagacgga cgcttcagta tcaacaatga agggttgctg gtgacggccg
     4501 gcggccggcc ggtgctcgac gatcggggca agacgatttc cgtgcccgag accgcccgag
     4561 gccggatccg aatcggtgaa gatggcaccg ttcgttccgg cgacgaggct ttcgcaagga
     4621 tcggccttgt cgacttcgca gaccgttcgc ggctctgcaa agtcggcgca aacatgttcg
     4681 aggccgtcgg aacgcgggct gaagccgttt cccccagact gcggacaaac tcattggaat
     4741 cgtccaccgt cgatccgacc aaagccatgg tggcgatgat cgaggtgtct cgagcatacg
     4801 aaatcaatgc caatctggtg agcatggccg acacgacgct gagccgcgcc gtgaatgata
     4861 tcgggcggat tcagtagatg ttgcgaggat gagctgaacc catgggtatc actgcgatgt
     4921 atgccgctgc gacggggatg aaggcgatgg acacgaagct caacgtcgtg gccaacaacc
     4981 tcgccaacgt cggtaccgtc ggcttcaagc gctcgcgcac gaacttcgaa gacctgatgt
     5041 accagaccct ccgtgagccg ggcgtcaagg acatcaacga tgagcccaac ccgtacggaa
     5101 tcctggtcgg actgggcgcc gctgtgtcag ggacgcagct tgatttccgc gtgggcagcg
     5161 cggagcagac ggacaagcct cttgacgtca tgatccaggg agagggcttt ttccaggtca
     5221 ccacgtatta caacggcgag gaaatcagag catacacgag ggcgggcaat ttcttccgca
     5281 acgccaacgg gcaactcgtg ctcggcaaca cggacggctc ggtgttggag cctcggatca
     5341 cactgggtga ggacgaccgg gagatcgaga tcgatgccgc cggtcgcgtc tcggccattc
     5401 agggcgccgg ccccgaccgg accgacatcg ggcagatcga gctggcccgg ttcatcaatc
     5461 ccgaaggact caagagcatc ggtcagaacc tgtacgtcga aaccgacgcc tccggcccgc
     5521 ccatcaccgg taacccgatg gagaacggta tgggcagcct gcagaaccgg atgctcgaat
     5581 tgagcaacgt tgagccggtg cgcgaactgg tggacctgat tttcacgcag cggggcttcg
     5641 agctgaactc gcagtcgatt cagggggccg acgagatgct gcgagtggtg gcgaatctga
     5701 gacggtagcc gtgaacggcg gccgcgaaca ccgcggcgaa ggcgaccaac aatggatatc
     5761 ggtcgtgacg acactgacgg atgaccacgg agcggaaacg gcagcgaggt gacagccagg
     5821 catgggtgac agtcggtagc gggtacaggg
//
LOCUS       DAOTPW010000302         5841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11637_length_5841_cov_5.342897, whole genome shotgun sequence.
ACCESSION   DAOTPW010000302 DAOTPW010000000
VERSION     DAOTPW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11637_length_5841_cov_5.342897"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            390..1277
                     /locus_tag="P5316_19565"
     CDS             390..1277
                     /locus_tag="P5316_19565"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR87214.1"
                     /translation="MRPARPTQKTTGRPQAGFTLIEVLVVVSIIALLIAILLPSLGRA
                     RSQARMVSCQSNLKQLTTAFLVYAAEYKNRLPGASKDDNADWLGGSNQGGPSLQPRNG
                     TVFRHMGRSTEAYTCPDDVIKRDYTTNDKLKPCDYSYTSNALVSGAATEFLGGAHYPT
                     KAPYDRDDHRFDMAPFEGVPVLIEEDPYWYLAKVDDSTWCNEDTIADRHLKFGSNPGQ
                     ANIGYHDGHVSRVRFKPPPSHLSSYPLAQYFNAKSMCIRTSGRKWVSGRSWQLSNGNY
                     ARYGFLPNGEDASTRGIQH"
     gene            complement(1474..4818)
                     /locus_tag="P5316_19570"
     CDS             complement(1474..4818)
                     /locus_tag="P5316_19570"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRR87215.1"
                     /translation="MVQRPTCLFVMFLGPLGILIAPGNDAQARQSLGDLTGPWQLLVD
                     DYLIADKNGTARTYHSLEKYPGNPVLLPTEAWEDSVAYIYGTVLPDETGTGYRMWYHT
                     LRPEDPDNDGSNILYATSPDGITWTKPNLGIRSWHGSTANNMIFVRPGQSGITSVIHT
                     PWNSDPNRQYTLMNFDVGGYWGAYSPDGIHTFDLSNNPVITDGGDVGQFMWDPFVRQF
                     RGYVKLNSYVNGLKRRSVGLTTTASIESWPPLALILEPDAFDDRWVPAGTVQRTHLYG
                     MSVFAYETMYLGFLWIFRATDAEGYYIGPVYAELVSSRDGVHWKREEQDRPPILPLGT
                     PGQWDDGQLYTAIAPVLNGDTLMVYYGACDNVHGYATKLLNCAIGLATLRKDGFVSID
                     AGPTGGTMSTRSLLGASGPLHVNYRTSGGWLKVEVLDADDQVVPGYDETECVPLQGDS
                     VDQIVTWQSHSELPADVRPLRLRFIMRNASLYSFMAGPDVQPLDSPAITRQPYDQTIA
                     PGGNACFVVEATGTSPLVYQWRKNGRDLVDGGRYGGTNAPTLTITTADLSDAAVYDCL
                     VSNPYGSIVSAKARLNVTTAEFIGLGTAPGATSSVVTGIATDGSVICGNSGGRPFIWT
                     AGGGIRDLGLPTDATSAGAAGVGVYNGNIVVAINSNASNYKAKCWEGNTAGQGAFANL
                     PQADTLEWTASGLGTNGDSELWISGSTINGGDGNGRQACRYRLSTGTVDLPVLPNNGH
                     DHSDFHAVSDNGCFAGQYQYMGTAPTGGARNPMKFTGASPCQPLNTLFGTPTTKNEAV
                     ARAVSRNGSVQGGWSYYINGVFLYMPCVWINSTTPTAVGFIPGGDSDNHGEILALSGD
                     GALAGGYSLYRNSPNPDGPREALIWDAAGGARQLQGWLSSQFGLNLAGWTLQEVRGIS
                     ADGQILTGNGNHNGVSEGWMLRFTAHDPPPPTITQQPLSQVVCPGDTAMFQLAATGQG
                     PLSFWWIKDGRALSDDGHYAGTDSPTLTISGITAADAGSYRCRITAGFNSIESDPANL
                     AIWPTVPADLDGDCDVDQDDVIIFEACAAGPGIPYPDACPLMPDTQGRVAADFDGDGD
                     VDQADFGAIQRCLTGHGTPADSHCRQ"
BASE COUNT         1104 a         1628 c         1926 g         1183 t
ORIGIN      
        1 caccgcatca tcaaaccggc ggatttacag catgttcaga ccggcgccga caaggggaac
       61 cgctttcttg cgggccgcaa cgagagcgta ctgtgccccc aaaagatgcg cgtaaaatgc
      121 gcaccaggta cgacggagga gccccaaacg attggaagcg cttgctgtgt tggtgaatac
      181 cctataccgg tatgagaacc aatagaacgt ctgtttctgt tgacgcatgc ctttgagacc
      241 gatataatca aatgcagtcg cgggtgcgta acctgccttg cgaaggcgcc gatggactga
      301 caagttgtcg gtgccgcgcc gagttggatc cgagcttttg tcagataacc tgtggtgcgg
      361 agcccgctgg aggacagagg gaacaaaaca tgcgaccagc acgcccgact cagaagacca
      421 ccggccgtcc ccaagcagga ttcacgctca ttgaagtcct ggtggtagtg tcgatcatcg
      481 cgctgctcat tgcgattctt cttccttcgc tgggacgggc caggtctcaa gcccgcatgg
      541 tcagttgtca gtccaacctc aagcagctca cgaccgcgtt tctggtctac gcggccgagt
      601 acaagaatcg tttgcccggg gcaagcaagg acgacaacgc cgactggctg ggaggcagca
      661 accaaggcgg tccgtcgttg cagccgcgga acggcacggt tttcagacac atgggtcggt
      721 cgacggaggc ctacacctgc cctgatgacg tgatcaagcg cgactacacg accaatgaca
      781 agctgaagcc ttgcgattat agctacacct ccaacgccct tgtgtccgga gcggccactg
      841 agtttcttgg cggggctcac tatccgacca aggcgccgta tgatcgggat gaccatcgct
      901 ttgacatggc gcctttcgaa ggcgtgcctg tactgatcga agaagacccg tactggtatc
      961 tggcgaaggt cgacgacagc acctggtgca acgaagacac catcgcggat cgccacttga
     1021 aattcggctc gaacccgggt caagccaaca tcggatacca cgacggccac gtttcgcggg
     1081 tgcggttcaa gcctcctcct tcacatttga gttcgtatcc gttggcccag tacttcaacg
     1141 cgaagtcgat gtgcatccgc accagcggcc gtaagtgggt cagcggcaga agctggcaat
     1201 tgtccaacgg caactatgcg aggtacggtt ttctgccgaa cggagaagat gccagcactc
     1261 gggggatcca gcactgaagg ttgcccgcag cgggcaggtc ttgtttcgag acaagcgcgc
     1321 aaggggcgca tctggggctt ggtcagcgat tctgcatgta gatgcgtggg aagggcgggc
     1381 cggtgttcga gctcgcggcg ggtctcgctc cgcgcggcaa cctggatggt gtcgaacctt
     1441 acacgcccgg cgatgcgacg ggcgttatcg ggcttattgt cggcaatggg agtcggccgg
     1501 ggtaccgtgg ccagtcaggc atcgttgtat tgcaccgaag tcggcctggt cgacgtcccc
     1561 gtccccgtcg aaatcggctg cgactcggcc ttgggtgtca ggcattagcg ggcaggcgtc
     1621 cggataaggg atacccggcc cggcggcgca tgcctcgaag atgatgacgt cgtcctggtc
     1681 cacatcgcaa tcgccgtcca ggtcagccgg aacggtgggc cagatcgcca gattggccgg
     1741 gtcggattcg atactattga agccggccgt gatcctgcag cgatagctgc ctgcatctgc
     1801 ggccgtaatg ccggagatag tcagtgttgg cgagtccgtg ccggcgtaat gtccgtcgtc
     1861 ggagagtgcg cgtccgtcct ttatccacca aaagctgagt ggtccctggc ccgtggcggc
     1921 gagctggaac atagcggtgt cacccgggca aacgacttgt gacaaaggct gctgcgtgat
     1981 cgtcggtggc gggggatcat gggcggtgaa tctgagcatc cagccctcgc tgacaccgtt
     2041 gtggttgccg ttgccggtca ggatttgccc gtcggctgag atgccgcgga cttcttgcag
     2101 cgtccagccg gccagattca gcccgaactg gctggacagc cagccctgca actgccgagc
     2161 gccgccggcc gcatcccata tcagagcttc tctggggccg tcagggttgg ggctgttgcg
     2221 gtaaaggctg taccccccgg ccagagcgcc atcgccgctg agagcaagga tttcgccatg
     2281 attatcgcta tcgccccccg ggatgaagcc gacggcggtg ggggtggtcg agttgatcca
     2341 gacgcacggc atgtagagga agactccgtt gatgtagtag ctccagccgc cctggacgga
     2401 accattgcgt gagacggctc tggccacggc ctcgttcttt gtggttggcg tgccgaagag
     2461 ggtgttcagc ggttgacagg gagaggcgcc ggtaaacttc atgggattgc gggccccgcc
     2521 ggtgggtgcc gtgcccatgt actgatactg tccggcgaag cagccgttgt ccgaaacggc
     2581 gtgaaaatcg gagtggtcat gcccgttgtt gggcaggaca ggcaggtcaa cggtaccggt
     2641 gctcaggcgg tagcggcaag cctgccggcc gttgccgtcg cctccgttga tggtgctgcc
     2701 gctgatccac aattcggaat cgccgttggt gcccaggccg gaggccgtcc attccagcgt
     2761 gtcggcctgc gggaggttgg cgaacgcgcc ttggccggcg gtgttgccct cccagcactt
     2821 ggccttgtag tttgaggcat tgctgttaat tgccaccacg atgttaccat tgtatacgcc
     2881 gacgccggcc gcgccggcgc tggtggcgtc ggtgggaagg ccaagatctc tgatgccgcc
     2941 gccggcggtc cagatgaacg gccggccgcc gctgttgccg cagatcacgg agccatcggt
     3001 ggcgatgccg gtgaccacgc tggacgttgc tcccggggct gttcccaacc cgatgaactc
     3061 agccgtggtg acgttcagtc ttgccttggc cgaaacgatg ctgccgtagg gattggacac
     3121 caggcagtcg tagacggccg catcgctcaa atcggccgtg gtgatcgtca gcgttggcgc
     3181 atttgtgccg ccgtaccgac cgccgtcgac aaggtctcgt ccgttcttgc gccactgata
     3241 gaccagcgga gacgtgcccg tggcctcgac gacgaagcac gcgtttccgc ccggagcaat
     3301 cgtttgatcg tagggttgcc gggtgattgc aggcgaatcg agcggctgaa catcgggtcc
     3361 ggccatgaag gagtaaagcg acgcgttccg catgatgaag cgcagtcgga gcggtcttac
     3421 gtctgcgggc agttcgctgt gggattgcca ggtgacgatc tgatcgacgc tatcgccttg
     3481 gagtgggacg cattcggttt cgtcgtagcc tgggacaacc tggtcgtccg catcgaggac
     3541 ttcgactttc agccagccgc ctgaggtccg gtagttgacg tgcaaggggc cggatgcacc
     3601 gagcaggctc ctggtggaca tggttccacc ggtcggtccg gcatcgatag agacaaagcc
     3661 gtcctttcga agagttgcca gaccgatggc gcagttgagc aacttggtcg cgtaaccgtg
     3721 aacgttgtcg caggcgccgt agtagaccat gagggtgtcg ccgttgagca cgggagcgat
     3781 ggcggtgtag agctgaccgt catcccattg gccaggcgtt cccaggggta ggatgggagg
     3841 gcggtcctgc tcctctcgtt tccagtgtac cccgtcgcgg ctgctgacca attcggcgta
     3901 caccggtccg atgtaataac cctccgcatc tgttgcccgg aagatccaga ggaagccgag
     3961 gtacatggtt tcgtacgcga acacggacat gccgtagaga tgggttctct gcacggttcc
     4021 ggccgggacc cagcggtcat cgaaggcatc gggttcgagg atgagtgcga gcgggggcca
     4081 gctttcaatg cttgcggtcg tggtgaggcc gacactgcgc cgcttcaatc cgttcacata
     4141 ggagttgagc ttcacatagc ctcggaattg tcgcacgaaa gggtcccaca tgaactgacc
     4201 gacgtcgccg ccgtcggtga tgacgggatt attggacaga tcgaaggtgt ggatgccgtc
     4261 cggcgaatac gctccccagt agccgccgac gtcgaagttc atcagcgtgt actgccggtt
     4321 gggatcggag ttccacggcg tatgaatcac ggaagtgatg ccgctttgcc ccggtcggac
     4381 gaagatcatg ttgttggccg ttgagccgtg ccaggaccgg atgccgagat ttggcttggt
     4441 ccaggtgatt ccgtcggggc tggtggcgta gaggatgttg ctaccgtcgt tgtcggggtc
     4501 ttcggggcgc aaggtgtgat accacatgcg ataccccgtt ccggtctcgt cgggcaggac
     4561 cgtcccgtag atgtaggcaa cgctatcctc ccacgcctcc gtgggaagga ggacagggtt
     4621 gccagggtac ttttcgaggg agtgataggt tcgggcggtg ccgtttttgt cggcgatgag
     4681 gtaatcatcg acaagtagct gccaggggcc ggtcagatcg ccgagggatt gtcgggcctg
     4741 agcgtcattg ccgggggcta tcaagatgcc tagtgggccc aggaacatga caaagagaca
     4801 tgtcggcctt tggaccacgt tcaatccctc cgatgacggg catctgagcg cgaggtggtc
     4861 gatccgtggc gggggttcat ccgcgcgtcc aacagaatcg tctccgcgct ttttcatcat
     4921 agcaaagcgg gagctgcgag gccatccggc gtgtccgggg ccgacggtgt ggcgggtgcc
     4981 ggagccggtc agcgacatgg aaggaaatac agagccaggc gaacgaggcg atgttcacgg
     5041 tgtctcccgc tcaatgtgag cggccaagcc gcagcatgcg cgcgggtgtt gtcggctttc
     5101 aacgtgccaa caggtgtgca accaggatcc ctgcatctct attagccagt ctaagccatc
     5161 tttttcagtt ttccaggcgc atcacgcgag ggtcagcagc ggtgcatctg cttcgaccac
     5221 cggtgtatca gtgaacgaaa cagaaacaag attgagtcaa ccgccgggcg ttacgttcag
     5281 gtcggttccg gagggttggt tcgtcgggac gagcaggaac aacgccgtca ttctatcggg
     5341 taaaacgcgt taagagctgt tctgcgcagt cttcagacgg ataggcgtcg gcgacgtgat
     5401 tccgtcagtc attttcgggt agaaaaacgg gtcagtgtgg caagaaatcg ttgacaacga
     5461 aggcggcctg tgatacacgg gcattgcggc gtgcggcact gagcctttcg cgctatccgt
     5521 gagcctgtcg atgagcaatg tggcggagaa catcgagttg aacagcagga gcggttccgc
     5581 gagatcggag tggcaccgag cggtttctta tccggcatca atctgaccgc gcgcccgtct
     5641 gagcaagttg ccaatcgtgt gatttcatgt gcggtagcgg cacagggtgg atggtgtgcg
     5701 ccattctcaa ccctatgtag cggaggtttc aactcatgga ctgttatgta gtcgaaaaaa
     5761 cttccagaag caccgagaca cagtcggcag ggcgagcggt tgcccgggcg ttttggctgg
     5821 ccgtgggcgt cctgttgccg g
//
LOCUS       DAOTPW010000303         5818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11688_length_5818_cov_5.464168, whole genome shotgun sequence.
ACCESSION   DAOTPW010000303 DAOTPW010000000
VERSION     DAOTPW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11688_length_5818_cov_5.464168"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..2003)
                     /locus_tag="P5316_19575"
     CDS             complement(243..2003)
                     /locus_tag="P5316_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650885.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRR87216.1"
                     /translation="MIAETSMKGLESFQLGRWDYLAFIAYFVVLSMIGYWAGRRKKSR
                     SDDYFLAGRSLPWYVVGSSFIASNISSEHFIGMVGAAFMLGICAALFEWGNILSFSIL
                     IWFFIPFLLASKVFTAPEFMERRFNPTLRNAFAIATVITNVLAFLAGVLYGGGVAIQK
                     LFGWDLWFAVTILAIAAGAWAIYGGLSSAAWADVFTVIVMVAGGMTVTILGLQSLSPN
                     HSVIDGFKTVIERNQATEGIWAEAVAQNVQNIVPGEETYNRLSVIQPATHKLIPWSSL
                     ILITFTVSIWYNVINQFMIQRVLGAKSMYHARMGIVFAGYMKILLPVIVVLPGLILFA
                     KNPEILKLPWDQIGPEADKGYVHMLQEFVPLGIRGLFLAALFGAIQSTVQAVLNSTAT
                     IFTLDIYKPMLRPTASEKNLVWTGRIAAIAFLGLAILLALLIARLGGGLFNYVQTLYA
                     FFAPPFGAIFLLGILFRRINGQGASAGVLLGFLTAIAIKLALYKQWIHDPVAAWLSPF
                     GNQAVVTWLVAIVACVIVSLLTPAPRPEQVTDELTFNWRKLNIFADLGRHWYTHVLLW
                     WALFVLIVGGLMLLFSGIWF"
     gene            complement(2143..3543)
                     /locus_tag="P5316_19580"
     CDS             complement(2143..3543)
                     /locus_tag="P5316_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRR87217.1"
                     /translation="MSSKPISEPADQVPPSAAVHLGVARRRIDPPSGIYARNWGAARH
                     DTAEGIHGSLLATVLVIRGQGSPDPLILASLDLGWWQQAEDEWLVRGALIESLCLRPE
                     RVMLSCIHTHAGASLCSGDADKPGGNLIQQYLHQLRDSLMDATREALDRMAPGTLSWT
                     TGRCDLAANRDLPDPLRNRILCGYNPAKPADDTVLVGRATDEKGRLLATIVNYACHPT
                     TLAWDNRLISADYVGPMRELVESHTEQAPCLFLQGASGELAPREQYTGDLAIAESNGR
                     RLGYAVLAALESMLPPRTKLAYAGVVESGAALATWRREPFDPSKVVLAEQVDVELPLK
                     PLPSEEELRRQLAECSDRVMAERIRRKMRVVHMVGSGPTTQMPAWIWRIGDAILVGQP
                     NEACSAFQTELRRMFPRKAVVVMNLVNGCCGYLSPPELHDLDIYQVWQSPFDRQALPC
                     LIETCRERISSMLPCA"
     gene            complement(3550..4374)
                     /locus_tag="P5316_19585"
     CDS             complement(3550..4374)
                     /locus_tag="P5316_19585"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRR87218.1"
                     /translation="MSAKERFDLTGKIALVTGGSRGIGRGIAIGLAEHGADVAIVYRA
                     AEAEAAGAAEAIRKLGRRAWIYRQDLAETDKLACLAERVWNDCGRIDILVNNAGMAYL
                     ERFEEITPEHWHRVLAVNLDAVFFLTQAIARRMIEAGIKGRIINLSSKNGFVAEAGLA
                     HYNASKGGVELLTQSLAIELGPFGITVNTIAPGIIETEIAGEFELDPAFFDYYKEHIP
                     LQHRFGSVEDCVGAAVFLASRAGAYMTGQHLIIDGGVLCEQVPRMQFMRASKLRRK"
     gene            complement(4489..5430)
                     /locus_tag="P5316_19590"
     CDS             complement(4489..5430)
                     /locus_tag="P5316_19590"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRR87219.1"
                     /translation="MQDTPPGPPRKPRSLRGVLPVFQTPFHEDESIDLVTLEREIDWL
                     YDCGADGIVMAMVSEVLRLSTDERELLAEHACRLGGEQGVVIISVGAESTYMAERYAR
                     QAESVGADAVMAIPPVSIAPLESEVLRYYERIIEAVEVPVIVQDASGYVGRPMPVELL
                     ARIRAAFGDRVAFKPEATPIGPRLSALREATGETAAVFEGSGGIALVDSFRRGIIGTM
                     PGADLIEGIVALWRALEARDERRIYRLSLPISALVAMQSGLDGFLAVEKYLLVKQGVF
                     ANTVVRGPVAFQLDEETREEVDRLFDQMMEAVRENEG"
BASE COUNT         1134 a         1846 c         1792 g         1046 t
ORIGIN      
        1 gcgtcggccc taccccccgc atgcccacgc aaagccgtgg gcatggcacc cggcaacttg
       61 ggctatcttg tcaagtcgcg gggatgagaa acaagacggg attcactgga agggtaccca
      121 agagcaaaga accgtgtgca ggggcaacag ctcacccggc cgcagcgtgg ttcctggctt
      181 cggcgacatc cggcgtttag ttggagacgt cgtcgcgacg ccgaccacga tagcgttctg
      241 ggtcagaacc agatccccga gaacagcagc atcaggccgc cgacgatcag gacaaagagc
      301 gcccaccaga ggagcacgtg cgtataccaa tgccggccaa ggtcggcgaa gatgttcagt
      361 tttcgccagt tgaaggtcag ttcgtcggtg acctgctcgg gacgcggggc gggagtcagc
      421 aggctgacga tcacgcacgc gacgatggca accagccagg tcaccaccgc ctggttgccg
      481 aaaggtgaga gccacgcggc gacggggtcg tgaatccact gcttgtacag tgcgagcttg
      541 atggcgatgg cggtcaggaa gccgagcagc acgccggccg aggccccctg tccgttgatg
      601 cgcctgaaca gaatgcccag caggaaaatc gcgccgaacg gcggggcaaa gaatgcgtaa
      661 agcgtctgca cgtagttgaa gagacctcca cccagtcggg cgatcaacag agccagcaga
      721 attgccaatc ccaggaacgc gatcgccgcg atacggccgg tccagacgag gttcttttcg
      781 gaggccgtcg ggcgaagcat cggcttgtag atatccaggg tgaagatggt agcggtcgaa
      841 ttgagcaccg cctggaccgt ggattggata gctccgaaca acgcggccag gaaaagtccg
      901 cggatgccca aaggaacgaa ttcctggagc atgtggacgt agcccttgtc ggcctcgggc
      961 ccgatctggt cccatggcag cttgaggatc tcggggtttt tggcgaacag gatcaacccg
     1021 ggcaacacca cgatgaccgg aagcaggatt ttcatgtagc cggcaaagac aatccccatc
     1081 cgggcatggt acatactctt ggcgccgagt acgcgctgga tcatgaactg gttgatgacg
     1141 ttgtaccaga tgctgacggt gaaggtgatg aggatcaggc tggaccaggg gatgagcttg
     1201 tgggttgccg gttgaatcac cgacaggcgg ttgtaggtct cctcgcccgg aacgatgttc
     1261 tggacgtttt gggcgacggc ctctgcccag atgccctcgg ttgcctggtt ccgctcgatg
     1321 acggtcttga agccatcgat gaccgaatga ttgggtgaaa ggctctgcag gccgaggatc
     1381 gtcaccgtca tgccgccagc caccatgaca atgaccgtga acacgtcggc ccaggcggcg
     1441 gagctcaggc cgccgtagat cgcccaggcg ccggccgcga tcgccaggat cgtgaccgca
     1501 aaccacaggt cccaaccgaa cagcttctga atggcaacgc ctccgccgta cagtacgcct
     1561 gccaggaacg ccagaacgtt ggtgatcacc gtagcgatgg cgaacgcatt ccgcagagtc
     1621 gggttgaatc gtcgctccat gaactcggga gcggtgaaca ccttggaggc cagcaagaac
     1681 gggatgaaga accagatgag aatgctgaat gacaggatgt tgccccactc gaacaaggcc
     1741 gcgcagatgc cgagcatgaa cgccgcaccc accatgccga tgaagtgctc ggagctgatg
     1801 ttcgaggcga tgaacgagct gccgacgacg taccacggca ggctgcggcc ggccaggaag
     1861 tagtcgtccg acctggactt cttccgccgc ccggcccagt agccgatcat ggacaagacc
     1921 acgaagtaag cgatgaaggc gaggtaatcc cagcggccaa gctgaaacga ctccaggcct
     1981 ttcattgagg tctcagcaat catgttggct cccggtttgt ggttcgcgga cgatcgggac
     2041 gacatcctaa cgtaccaggc cttcggcccg agctaacggc tcctggcaag cgccagtatt
     2101 taccattgct gctggcctct accttacccc cctacacaca cctcatgcgc acggtaacat
     2161 cgaactaatc cgctcccgac aggtttcgat caggcacggc agggcctggc ggtcgaaggg
     2221 gctttgccaa acctggtata tgtccaggtc gtgaagctcc ggtgggctca ggtagccgca
     2281 gcagccgttg acgaggttca tgaccactac cgctttccgg gggaacattc ggcgcagctc
     2341 ggtctgaaac gccgagcagg cctcgttcgg ctggcccacg agaatcgcgt cgccgatccg
     2401 ccagatccag gcgggcatct gggtggtcgg cccggagccg accatgtgaa ccacgcgcat
     2461 cttgcggcgg attcgctcgg ccatgacacg gtcggagcac tcggccaact ggcgacgcag
     2521 ttcctcttcg cttggcagcg gcttgagcgg caactcgaca tccacctgct cggccagcac
     2581 cactttcgac gggtcgaagg gctcgcggcg ccaggtggcc agggcggcgc ccgattccac
     2641 gacgcccgca taggcaagct tcgtccgtgg cggcagcatc gactccaacg cagccagcac
     2701 cgcgtagccc agccttcggc cgttcgattc ggcgatggcc aggtcgccgg tgtactgctc
     2761 gcgaggagcc aattcccccg aagccccttg gagaaacagg cagggggcct gctcagtgtg
     2821 tgattcgacc agctcgcgca tcggtccgac gtaatcggcc gagatcaggc gattgtccca
     2881 ggccagcgtg gtcggatggc aggcgtaatt gacgatcgtg gccagcagac ggcctttttc
     2941 gtccgtggcc cgcccgacca gaacagtgtc gtcggccggt ttcgccgggt tgtagccgca
     3001 gaggatccga tttcgcaggg ggtcgggaag atcacggttg gcggccaggt cgcatcgtcc
     3061 ggtcgtccag gaaagcgtgc cgggcgccat gcggtccaag gcctctcgtg tcgcatccat
     3121 gaggctgtcg cggagttggt gcagatactg ttgaatcagg ttcccgccgg gcttgtcggc
     3181 gtcgccgctg cacagcgagg caccggcgtg ggtgtggatg cacgacagca tgacccgctc
     3241 gggacgcagg cacaacgact cgatgagcgc ccctcgaacc aaccactcat cctcggcctg
     3301 ctgccaccag ccgagatcca gactggccag aatcagcgga tcgggcgagc cctggccgcg
     3361 gatcaccaat accgtcgcaa gtagcgagcc gtgaatgcct tcggcagtgt cgtgcctggc
     3421 agccccccag ttgcgggcgt agatgccgga gggggggtca attcgtcgcc tggccacgcc
     3481 cagatgaact gccgcagagg gcggcacctg atcggccggc tcgctgatcg gtttgctact
     3541 catggcattt catttcctgc gcagcttgct tgccctcatg aactgcatcc gcggcacctg
     3601 ctcgcaaagc acacctccgt cgatgatcag gtgctgcccg gtcatgtaag cgcctgcccg
     3661 cgaggccagg aacaccgccg cgcccacgca gtcctcgacg cttccgaagc ggtgttgcaa
     3721 cggtatgtgt tccttgtagt agtcgaagaa cgcgggatca agttcgaatt cgccggcgat
     3781 ttccgtctcg atgataccag gggctatcgt gttgacggtg attccgaagg gccccagctc
     3841 gatcgccagc gactgagtga gcagctccac gcctcccttc gaggcgttgt agtgagccag
     3901 accggcctcg gccacaaagc cgttcttcga agagagattg atgatccgcc ccttgattcc
     3961 ggcctcgatc atccttcgag cgatggcctg cgtgaggaag aacaccgcat cgagattgac
     4021 cgcaagcacc cggtgccagt gctcgggcgt gatttcctcg aatcgctcca ggtaggccat
     4081 gccggcgttg ttgacgagga tgtcgatccg cccgcaatcg ttccacacgc gctcggccaa
     4141 gcaagccagc ttatccgtct cagccagatc ctgcctgtag atccacgccc ggcgaccgag
     4201 cttgcggatc gcctccgccg cgccggcggc ctcggcctcg gctgcgcgat agacgatggc
     4261 cacatccgcg ccatgttcgg ccaggccaat ggcgatgccg cgtccgatgc cccggctgcc
     4321 gccggtgacc agggcaatct tgcctgtgag gtcgaatcgt tctttggcgc tcaaagactt
     4381 gtcctccgct cgaaacgcac atggtagacg atgaaagctg tcagcgatca gccccaaaaa
     4441 ctgatgactg atagctgacg gcttgattca tccttcaacc ttcggccctc atccttcatt
     4501 ctccctgacc gcctccatca tctggtcgaa aagacgatcg acttcctccc gcgtttcctc
     4561 gtcgagctgg aatgccaccg gacctcggac cacggtgttg gcgaagacgc cctgcttgac
     4621 cagcaggtac ttctcgacgg ccaggaagcc gtccagccct gactgcattg ccaccagcgc
     4681 tgagatcggc agtgacaggc ggtagattcg tcgctcgtcg cgggcttcca gggcccgcca
     4741 caaggcgacg atgccctcga tcaagtccgc ccccggcatg gtcccgatga ttccccggcg
     4801 aaagctgtcc acgagggcga ttccgccgct gccctcgaag accgccgcgg tctcgccggt
     4861 cgcctcgcgc agggcggaaa ggcgcggacc gattggcgtc gcctccggct tgaaagccac
     4921 gcgatcgccg aaagcagctc gtattctcgc cagcagttcc accggcatcg gccgtccgac
     4981 gtacccgctg gcgtcctgca cgatcacggg aacctccacc gcttcgatga ttcgctcata
     5041 gtagcgaagc acctcggact cgagcggcgc gatcgacacc ggcgggatcg ccatcacggc
     5101 atcagcgccg acggattcgg cctgtcgggc gtagcgctcg gccatgtacg tgctctcggc
     5161 tccgacgctg atgataacca ctccctgctc gccaccgagg cgacatgcgt gttcggccaa
     5221 cagctcgcgc tcatcggtgg acagccgcaa cacctctgag accatcgcca tgacgatgcc
     5281 gtcggccccg cagtcgtaca gccagtcaat ctcccgctcc agggtcacca ggtcgatgct
     5341 ctcatcttcg tggaaagggg tctgaaacac cggcagtaca cctcgaaggg agcgaggttt
     5401 tcgcggcggg ccggggggtg tgtcttgcat cgcggcgtct cctgacaaga tagcggacga
     5461 cattctacta tgcttcaccc gccttgccac ccttgccaaa ctccggtgcg tgggtatcct
     5521 ctggatgctc ttgtttcggc ctttgcgcag accacagagc ctttgtgaga agccgcaggg
     5581 ccgggtggta gggcagggtc aaatccgcgg cggcggacga ggcctgccat atattcatgc
     5641 ttgaatcggc aggtctccgc cgcctttggc ggcttcgacc acgccttacg ctctgctcac
     5701 acatgctctc agatagaaag ggacgccaat gaaccgtgaa ttcgcactgc gtaccgtctc
     5761 cgaaagagtt gtgaccgtcg tcgctgccac aatagccttc accgccttgg ccggttgc
//
LOCUS       DAOTPW010000304         5761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11824_length_5761_cov_5.997371, whole genome shotgun sequence.
ACCESSION   DAOTPW010000304 DAOTPW010000000
VERSION     DAOTPW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11824_length_5761_cov_5.997371"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="P5316_19595"
     CDS             complement(<1..457)
                     /locus_tag="P5316_19595"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRR87220.1"
                     /translation="MEWLSIWGMAVGLAMDAFAVSIAVGLTVGKVTPRHVFRIAWHFG
                     LFQFMMPVIGWWAGSRVAGYVADWDHWLACGLLCFIGGRMLFGAFERQDQRTQSDPTR
                     GWLLVTLSVATSIDALAVGLSTAFLNVTIWLPSVIIGLVACAFSAIGITF"
     gene            complement(612..1868)
                     /locus_tag="P5316_19600"
     CDS             complement(612..1868)
                     /locus_tag="P5316_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87221.1"
                     /translation="MTPHGARLWLAVLNIAALLIAFPTPTPAQSRPTRAVADRDRAVD
                     DSFEHLFEEVGKLRLNTGQELRCVLGRSADDELAVRSALRQKCQSWHPYRHARGLLEV
                     DAWLPVSALDRTLEDLIQNRLRNANGPPDMTITWAKSNPPAVLATGRYLPSSSLVNEQ
                     LGWRHCSPWQTALAQKAAAADARAAMLVRMGYCKLPDGRELGFILHREKKLKVAVNSL
                     LKEAPAGEAVFDPTGVCRVTMKLTQPELLRLLQDAGRAVDGFERSELFKSVDFPATDN
                     LEAEGYAVTPPVPSAYKPPLKKGTEPPRPDWADRFLVVQGAGRAPSDATNDTGATDWA
                     ERAARIEAHRQLWMQIEDLPLDDGETVGLRMQKNPGLVFAMSGLDRFIAGANRPLRTD
                     DGAVTVNVGIHLQTVWRIVSTIERAR"
     gene            complement(2368..2658)
                     /locus_tag="P5316_19605"
     CDS             complement(2368..2658)
                     /locus_tag="P5316_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87222.1"
                     /translation="MIQCSECEFFVRGPAGQVGFKCDPFSTIKEPECLVKWQLIKLDM
                     MVQSYQATVAMYRRLAPLQEKMFRHMEREINEIDEADRWKIDQDEDEENENA"
     gene            complement(2860..3564)
                     /locus_tag="P5316_19610"
     CDS             complement(2860..3564)
                     /locus_tag="P5316_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87223.1"
                     /translation="MAHQTFLPTRPIGTRFGRWIGAALLLHFAYVVGLINLVRQSPGE
                     VLWLCHISLLLAGLGLVTQSVRLVAIACTAVALPHTFWLIDSLSGLLLGWFPLGATAY
                     ILTTDTWTRIATAHHFYLAPLLLVIVWRHGSFSRLALPAASLLFLLLSVTCRVAVSPA
                     LNVNYAFFAVPAADHPFVTWVNSLDAIPYLLLINAIVTGVMFAPVALLMTCHAQKTTH
                     AQNNRSVALPALQAPQ"
     gene            complement(3772..4146)
                     /locus_tag="P5316_19615"
     CDS             complement(3772..4146)
                     /locus_tag="P5316_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87224.1"
                     /translation="MKRRLPLVLVVALFPAACCAPCGEREIQQGLARRCSNLRKTTGW
                     LIEAEQRRPAQLEHTCRIIQEQCRHDARKTCVDNPATAGQWTEEEFARWQKRAPIYLQ
                     RTIEELCGDPENICRTLPMIVN"
     gene            complement(4636..4902)
                     /locus_tag="P5316_19620"
     CDS             complement(4636..4902)
                     /locus_tag="P5316_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87225.1"
                     /translation="MTDEVFAVRIEEARRKIETLPEDQREPLLRLLHETLQRQSDLKM
                     NFSKLRYLLDDWRVKMKYMAFDLEATKRELAELRRRQDNSGPQA"
     gene            complement(5100..>5761)
                     /locus_tag="P5316_19625"
     CDS             complement(5100..>5761)
                     /locus_tag="P5316_19625"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRR87226.1"
                     /translation="PVMVHRGDRTAARENTLEAYAAAMDRGADGVEIDIRRSKDGVLY
                     LHHDDTAGRMIPGEGKLKDMTYYEILSRGMTKTFGTADKNTRVPTLAAFIVLARQRCM
                     LLHLDVKEAGLQDDIIHMFEEADIWDHVVEVNAGNAERIRAHPKVKLLPYKGWWPEGE
                     HAEDPAAAKDVMGKAGVMVFTKEPGAAVKMLNKPVPSAPVPLPRNLRVMWTPEGPIPQ
                     L"
BASE COUNT         1059 a         1672 c         1894 g         1136 t
ORIGIN      
        1 caaaagtgat accgatggcg ctgaaggcgc aggcgaccag cccgatgatc accgagggca
       61 gccagatggt gacgttgagg aacgcggtgc tcaggccgac ggccaaagcg tcgatgctgg
      121 ttgccacgga gagggtgacc aggagccaac ctcgcgtcgg gtccgactgc gtgcgctggt
      181 cttgcctctc gaatgccccg aacagcattc tgccgccgat gaagcacagc agtccgcagg
      241 ccaaccagtg atcccaatcg gcgacatagc ccgcgactct ggagccggcc caccagccga
      301 tgaccggcat catgaattgg aagaggccga agtgccaagc gatgcgaaaa acgtgccggg
      361 gcgtgacctt gccgacggtc aagccgaccg cgatggaaac ggcaaatgcg tccatcgcca
      421 agccgacggc cattccccat atggacaacc attccaacag atgatctccc gcgagttcct
      481 gtttcgaagt cgccggatgc cgccgcatcc ttccgattcg caggacagtc tcaggcgaag
      541 tgcgtcggaa ggcaagtcgt ttcggtcact ggctgccgag aaccggcggt ttgcgagagg
      601 tcggcggtgc gtcaccgggc tcgttcgatg gtcgacacaa tccgccagac ggtttgaaga
      661 tggataccga cgttgacagt aaccgctcca tcatcggtgc gaagcggtcg gttcgcaccg
      721 gcaatgaatc gatcgagacc cgacatggca aacacgagcc ccgggttttt ctgcattcgc
      781 aggccgacgg tttcgccgtc atcgaggggc agatcttcga tctgcatcca gagttggcga
      841 tgggcttcga tgcgggctgc tcgctcagcc cagtcggtgg cgcctgtgtc attggtcgcg
      901 tccgagggtg ccctgccggc cccttggacc accaggaagc ggtcggccca gtcgggccgc
      961 ggcggttcgg ttcctttctt cagcggcggc ttgtacgcgc tcggtacagg cggggtgacg
     1021 gcataccctt ccgcctccag attgtcggta gcagggaaat cgacggactt gaaaagctcg
     1081 cttcgttcga aaccatcgac ggcacggccg gcgtcctgca acagcctgag cagttcgggt
     1141 tgcgtgagct tcatcgtgac gcggcacaca cctgttgggt caaataccgc ctctccagcg
     1201 ggcgcttctt tgagcaggct gtttacggcg actttgagct tcttctcgcg atgcaggatg
     1261 aatcccagct cacggccgtc cggcagtttg cagtacccca tgcgaacgag catggccgcg
     1321 cgtgcatcgg cggccgcggc tttttgggct agcgccgttt gccagggact gcagtgccgc
     1381 caaccgagct gttcgttcac gaggctgctc gaaggcagat atcggccggt ggcgaggacg
     1441 gcgggcggat tcgacttggc ccaggtgatg gtcatgtcgg gcgggccgtt cgcgtttctc
     1501 aatcggtttt ggatgaggtc ttccagtgtg cggtcgagag ccgaaaccgg caaccaggca
     1561 tcgacttcga gcaatccgcg ggcgtggcgg tacggatgcc aggattggca cttctgacgt
     1621 agcgccgacc ggacggccag ctcgtcatct gctgagcggc ccaagacgca gcggagttcc
     1681 tgtccggtat tcaggcggag tttcccgacc tcttcaaaca gatgttcaaa cgaatcatcc
     1741 acggcgcggt cgcgatcggc gacggcgcgg gtgggtcgcg actgggctgg tgtgggcgtc
     1801 gggaaagcga tcagcagtgc cgcgatgttc aataccgcga gccacaggcg ggcgccatga
     1861 ggcgtcactg gttcagatcg gttggcatga gcgcgtcggg ccatgacgtc tacgtctctc
     1921 gcatacctgc ccggttgaag ccggcatcct ggcggggcgt gtcgcagggc cggtggagtg
     1981 attgcctggc gatccgtaaa tgctgtatcg gaaaagccct gagagcccat cataatgacg
     2041 gtggctttgt ccgggctgtc aagcgacgcc gtgttcctgc gtgacggctt gccgcctgga
     2101 ggcgttgagt ccgcacgccg aggctcagtt atacggaccg gccgccggag gcgacagatt
     2161 gaatgcccgc gcttatcaag gcgtcgatcg ccagtgctga tatgggcgat ttcggccaag
     2221 atgcggtgga gagccgggtt ggcggttcat cgggcggtca gacgccacgt ttgggtctgg
     2281 tattgtccga tgcaacctgt gacgaaaact gagcaacctg cacgggcccg agacagaagc
     2341 ggcggcactc ctcgcggctg accgccttca ggcgttctcg ttctcttcgt cctcgtcctg
     2401 gtctatcttc catcgatcag cctcgtcgat ctcgttgatc tctcgttcca tatgccggaa
     2461 cattttttcc tgaagcggag ccagtcgacg atacatggcg accgttgcct gatatgactg
     2521 gaccatcatg tcgagcttga tcaactgcca tttgacgagg cattcgggtt ctttgatggt
     2581 gctgaacggg tcgcatttga agccgacttg gcctgccggg ccccggacga agaattcgca
     2641 ctcgctgcat tggatcatga tttgctcccc ggtctcttgc cgcatttgcc ggtcgtctca
     2701 aggcgccgac cggtgatcat tatacatccg caagccgaaa ggtgtgcaat tgcggcgtct
     2761 cattcgttgt gcaaaggggg caaggggccg gccgggtggg tgcggcctgt tgacccggtc
     2821 tgtctcggct gcggctttgg ggaccgacct gaacctgaat cattgggggg cttgaagagc
     2881 cgggagcgcg acggaccggt tgttctgcgc atgtgttgtc ttctgagcgt ggcaggtcat
     2941 cagcagcgca acgggcgcga acatcacacc tgtcacgatt gcgttgatca acaacagata
     3001 aggaatcgca tcgaggctgt tgacccaagt gacgaacgga tggtcggctg ccgggaccgc
     3061 aaagaaggcg taattcacgt tgagcgccgg cgatacggct acccggcagg taacgctgag
     3121 cagcaggaag agaagcgaag ccgccggcag cgccagccgg gagaacgaac cgtgccgcca
     3181 gacgatcacc aacaacagcg gagcgaggta gaaatgatgg gcggtcgcga ttcgagtcca
     3241 agtgtcggtg gtcagaatgt aggccgtagc tcccagtgga aaccaaccga gcagcagccc
     3301 ggagaggctg tctatgagcc agaacgtatg cggcagggca acggcggtac aggctattgc
     3361 caccaggcgg acggactgtg tgaccagccc gaggcctgcg agaaggaggc tgatgtggca
     3421 aagccacaag acctctcctg gtgactgtct gaccaaattg ataagcccga ccacgtacgc
     3481 gaagtgcagc aacagtgcgg ccccgatcca ccggccgaac cgggtgccaa taggccgcgt
     3541 cggcaggaag gtctggtggg ccacggtgtc ctcaactttc cgaggggcga tccgtaacaa
     3601 tcccaacacc aagctatcaa cctccatgct acaccagcaa cagcctgatg ccaagttttt
     3661 gccgcgtcac caaggcagta ccagccaaga cgggaagaaa gagagggttc cgggggaagc
     3721 gtcgcgcgtc gcgcacggct cattaccgaa ccggtaatgg tccgtgggag gtcagttcac
     3781 gatcatgggc agggtgcgac agatgttttc cggatcgccg cagagttcct cgatggttcg
     3841 ctggaggtag atgggggccc gcttctgcca gcgggcaaat tcttcctcgg tccattgacc
     3901 ggcggtggcc gggttgtcga cacaggtctt ccgcgcgtcg tgccggcatt gttcctggat
     3961 gatgcggcag gtgtgctcca gttgagccgg ccgacgctgt tcggcctcga tcagccatcc
     4021 ggtggtcttg cgcaggttgc tgcatcgacg ggccaagcct tgctggattt cgcgttctcc
     4081 gcagggggca caacaggcgg cgggaaacaa ggcgaccacg agaaccaacg gtaatcgacg
     4141 tttcattgac ggactccaac atcggccgag tcgccccgag cgccatgcgc tgagcggggc
     4201 ggtttcctcg ctccgtaggg attataccga caagctgggc cggcgccgcc caagtacgag
     4261 ccgggggtga ccggttgctg ttgggtgatc ctaaggcgga tcgccttttt gcgggccgcg
     4321 gcccaagcgt tctgcgatcc ggaaacatgc gcgcaagccg cacacatatc cggccgtaag
     4381 gttctaacca tcggccaggt cacggcttat cccagcgtcg cagacggcgg cgttggggga
     4441 aggacggcaa atacggaata ccgagaatgg ggtgtcgttg cgaacgacga aacacgccgg
     4501 cggtggtgtt tcggtgcgtg ccaagagact cgggaggaag gccgctccct ggtctgcata
     4561 ccgatgtccc tggtcggttc tgtgcaactg gagggttccg tctttccggc ggccggcggt
     4621 cgggccggtc gggtattacg cttgcggtcc cgagttgtct tgtctgcggc ggagttcggc
     4681 cagttcgcgt ttggtggcct cgagatcgaa ggccatgtac ttcatcttga cccgccagtc
     4741 gtccagaagg taccgcagct tggagaagtt catcttgaga tccgactgcc gctgcaaagt
     4801 ctcatggagg agcctgagca atggctcgcg ctggtcttcg ggaagggtct cgattttgcg
     4861 gcgggcttcc tcgatccgca cggcaaatac ttcgtcggtc atggggctct ctcccttccc
     4921 ggccgttggg cagtgattgc tgtcgcacca acgacgctac ttcaatttta tgattgcgtg
     4981 cagggcaggg tcaagaaaaa acgggtctcg cggagatggt ccgatagggt gtggagactg
     5041 ccggtctgga acgtccattg gtccgcagcg gcaggcgccg ccacaagagc ccaagaccct
     5101 cacagttgcg gtatcggacc ctcgggggtc cacatgacgc gcaggttcct gggcagcggc
     5161 acgggggcag acgggacggg cttgttgagc attttgacgg ctgcgccggg ttcttttgtg
     5221 aagaccatga ctccggcttt gcccatcacg tctttggcgg ccgcggggtc ctccgcgtgc
     5281 tctccctcgg gccaccaacc cttgtagggc agcagcttga ccttggggtg ggcgcggatg
     5341 cgctcggcgt tgccggcgtt gacttcgacg acgtgatccc agatgtccgc ctcctcgaac
     5401 atatgaatga tgtcatcctg caaacccgct tccttgacgt ccaagtgcag gagcatgcag
     5461 cgttgtcggg ccagcacgat gaaggccgcc agcgtgggca cgcgggtatt cttgtcggcg
     5521 gtgccgaagg ttttggtcat gccgcggctg aggatttcgt agtaggtcat atctttgagc
     5581 ttgccctcgc ccgggatcat gcggccggcg gtatcgtcgt gatgcaaata gagcacgccg
     5641 tctttgcttc ggcggatgtc gatttcgacg ccgtcggccc cgcgatccat cgcggcggcg
     5701 taggcctcca gggtattctc gcgggccgct gtgcggtcac ctcgatggac catcaccggc
     5761 g
//
LOCUS       DAOTPW010000305         5758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11834_length_5758_cov_5.991233, whole genome shotgun sequence.
ACCESSION   DAOTPW010000305 DAOTPW010000000
VERSION     DAOTPW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11834_length_5758_cov_5.991233"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="P5316_19630"
     CDS             complement(<1..319)
                     /locus_tag="P5316_19630"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR87227.1"
                     /translation="MEPNSAPRQAITYLPKHWQKLTVFARQPGALLDNDVVERTLKRA
                     ILHRKNALFYRTLHGAHVGDLFMSLIHTCQSDEIDPFDCLTELRHRAVELAAQPEHRI
                     PRNY"
     gene            1162..1734
                     /locus_tag="P5316_19635"
     CDS             1162..1734
                     /locus_tag="P5316_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRR87228.1"
                     /translation="MIRDLVLANRSYRRFQQSHSIDMATLRELIELATLCPSSGNLQP
                     LRYVLSCEPDKNAKIFQHLAWAGYLTDWTGPTQGERPSAYIIILGDSQACHSINCDHG
                     IAAQTILLGAVEKGLGGCIIGSIQREELRRALDIPERYQILLVIALGKPAEKIVLEPV
                     TSESDIKYYRDDGGVHHVPKRSVDQVILGL"
     gene            2292..4097
                     /locus_tag="P5316_19640"
     CDS             2292..4097
                     /locus_tag="P5316_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87229.1"
                     /translation="MSELRKKAKRAQLRLWCNRWFSTLGWSLAGGAALFVLLVLIDRL
                     WLVRPDAEVLTGWVCLGLLGASVVASAVWTFLTRETLSVAAAALDDAAGLRERISSAI
                     YCEKMDDPFGQAVVADAHRVTHGLTVGRHLPLRFPQSASYAGPSLLVALLVFWLFPVV
                     DLAGRQEALQKQLEQQKRVERAAALVQPLIDKQVETLRKRYPALEQEMQQLAALKNAP
                     PATRPEDVAKDPIKQVNNLARQIENKKENPDLAKIEEFQKIAQKLEARQRGDSAVSKL
                     VQAMARGDYKSAQQALEEMKLDLMKAPKTDEDKQKAEELRKQINKLGDQLKELAENDK
                     KLQQELAKTGMKPDEIARALEHLKKQDLDALKEQLKKQGLSEKDIQKLANQLKKCQGG
                     CQMAGRLGDSLKQAAQGQPGQQGSGQGSEAGFTDAADQLSDMEALQQEMNQLTAAASD
                     LQQMQDQLSQAGQCSGQCDGMGLGEGQGQLKESQSGMGPLGQGRGGIAPKTETGAKTT
                     AQKAKVESHPGAIISTQFVHGEQIAGQVTDEFVEAVISKEREVSDAINREAIPRQYHK
                     SVSKYFSDAREGLPADRVKAVENKFEGEKPAGEAK"
     gene            4099..5127
                     /locus_tag="P5316_19645"
     CDS             4099..5127
                     /locus_tag="P5316_19645"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRR87230.1"
                     /translation="MGGFSFPRAQAISCALAAAACAFLSGCDQSSDKPAVTLYTSVDE
                     PFAREVIAAFEKQSGIRVAIKIDSEAGKTTGLVRRIDAERNRPQADVFWSSELFNTIK
                     MGRQGLLAEYRPPAEGIPDRYKDPAGRWTAFGMRARVLAFNTSKVKPEELPTRWREIA
                     NSRWAGVLGVADPRFGTTRGHFAAFLAAWGEPTYAEFVEQLSQTLGDKLQDGNAAAAR
                     AVARGELHICATDTDDVYVRQDAKEPIDMVFPDMGDGGTLLIPNSVAMIANAPHPDEA
                     KRLIDFLTSEQTERMLARSPSRNYPVRESLRKELSITLPPEAKLTFEQIADAMDKAIE
                     LAGRKLIK"
BASE COUNT         1147 a         1644 c         1898 g         1069 t
ORIGIN      
        1 ggtagttcct gggtatccgg tgttcgggct gggcggcgag ctctacggcg cggtgccgca
       61 gttcggtcag gcagtcgaag gggtcgattt cgtcgctttg gcaggtgtgt atgaggctca
      121 tgaacaggtc gccgacgtgg gccccgtgca gggttctgta gaacagggcg ttttttcgat
      181 gcaggatcgc ccgcttcaga gtccgttcga caacgtcgtt atccagcagg gctccgggct
      241 gacgcgcgaa gacggtcagc ttctgccagt gcttgggcag gtaggtgatg gcctggcggg
      301 gggccgagtt gggttccacc ggccggtcct cgaactgccg cgtgcatcag gttcggagct
      361 gctccatgac cgggcccgaa cgcttccacg cttacaagag caccatggtg gtacacacgg
      421 cagtggcaca ctggccggcc actcacccgc gatacgaggg caagagcctc tcaccggccg
      481 gcttgttcca ggtcgctcag cgaccctggc gggctgccac ggtacgctgc ggtctccgcc
      541 ccgaacgaca agccccgatc cgagccaacg ccgccggtgt tcacgccgac gctgcccgcc
      601 ggcgacgtcc acgatcgctc aagcagcctt ccagtgcctg ggctgagtcc tgcttgctga
      661 attgcccgtg ccaatcttca tgcgggaccg ccgccgcaag cttttcggca tcgaaaagcg
      721 ggcactttga gggttgcatc gtaagcgact ctgtctgctt gccttttgtg gcagaggctg
      781 atgttttcca ggacgcaggt cggtcgtggg tttgctgccg gctgcaccgc gaaccgtgag
      841 cgtcgaggcc cgaaggcgtt ctcggccgga gacttacgtt tgctgatagc gccgcctgct
      901 cgctgaacgg cgacggactt acaggagggt gtatacgagc ggaaaggcgt ggaaagggat
      961 gataccgccg gccaagcgtg ccggcggtgg ggtcggcatg aggctttttc acgcccggtg
     1021 gcagggcacc cgcagcggag aaggaaagag atgctgacgg tctcttgttg cctgggacgt
     1081 gaggcgacac taggattcct gcgaacagga acgggcgagt gtgagggttt gtctgccggg
     1141 gtccgcggcg tggagaatgc gatgattcga gaccttgttc ttgccaacag aagttaccgt
     1201 cgttttcagc agagccattc catcgacatg gccacgttac gggaactgat tgaactggcg
     1261 acgttgtgcc cgtcgtccgg caacttgcag ccgttgcgat acgtcctttc gtgcgagccg
     1321 gacaagaacg ccaagatctt tcaacacttg gcttgggccg gatacctgac cgactggacg
     1381 ggtccgacgc agggcgaacg cccatcggcg tatatcatca tccttggcga ctcacaggct
     1441 tgtcacagta tcaactgcga tcacggcatc gctgctcaga ccatactgct tggggcagtc
     1501 gaaaaaggtc tcggcggttg catcatcggc tcgatccagc gggaagaact tcgaagggcg
     1561 cttgacattc cggaacgcta tcagatcctg ctggttatcg cactcggcaa gccggccgag
     1621 aaaatcgtcc ttgagccggt gacgtcggaa agcgacatca agtactatcg cgacgacggc
     1681 ggcgttcatc atgttcccaa acgatcggtg gatcaagtga tcctgggact atagcacggc
     1741 tcaggggtcg ggggccggtc gtcggctcgg ccgcatcgtg ccgaccagcg gctctgcccc
     1801 ggcctgtctc catttccggc catcgttcac cgccgtggta agacatcctt cggcgggaca
     1861 tgagcaactc gcgaccgctc gccaaacgcc gacaggtgaa acaggtagaa ggtaaactcc
     1921 ttgcatgtgc ggcggcgggt tggcctggtg gccgtgctca tccctttcgg tcttcgggca
     1981 cttgcccgga gccgagcctg cgggaggtcg ccggctcata agacactgtc aggtccgcgt
     2041 ttcctggtct ttctgttgcg atggtggtga cccggatggt ctgattctat ccctggaacc
     2101 ctgaacaaaa gctgtcccgg agcgtctaat gccgggagga ttgcgtaaga tccggtctac
     2161 cagcgttgtc tattggtatg ccagagggta ccatgaattg gtgcgtcgag gcggccgata
     2221 gcaggacagc gggggggcgg gcccggatcg acgccgaacc gggcaggggg atgttgacga
     2281 gggactgagc catgtcggag cttcggaaaa aggccaagcg ggcccagttg cggctgtggt
     2341 gcaatcgctg gttctccacg ctcggatggt ctttggcagg gggggcggcg ctctttgttc
     2401 tccttgtgct gatcgatcgt ctgtggctgg tgaggccgga cgccgaagtg ctgacgggtt
     2461 gggtctgtct ggggttgctg ggcgcctcgg tcgtcgcctc agcggtctgg accttcttga
     2521 cccgtgagac gctgtcggtt gccgcagccg cgctggacga tgccgccggt ttgcgggaac
     2581 gcatcagcag cgcgatctac tgcgagaaga tggatgaccc cttcgggcaa gccgttgtgg
     2641 ccgatgccca cagggtaaca catggcctga cggtaggtcg tcatttgccg cttcgtttcc
     2701 ctcagtccgc cagttacgcc ggcccaagcc tgctggttgc cctcttggtt ttctggcttt
     2761 tccccgttgt cgacttggcc ggcagacagg aagctctgca gaaacagctt gagcaacaga
     2821 agcgcgttga gcgcgccgcc gcgctggttc agcctttaat cgacaagcag gtcgagactt
     2881 tgcggaagag atatcccgct ctggaacagg agatgcagca actggccgcc ttgaagaacg
     2941 ctccaccagc cacgcggccc gaggatgttg ccaaggatcc tatcaagcag gtcaacaatc
     3001 tcgctcggca gatcgagaac aagaaagaga accccgatct ggccaagatc gaggagttcc
     3061 agaagatcgc ccagaagctg gaggctcggc aacgtgggga cagcgcggtc agcaagctgg
     3121 ttcaggccat ggcccggggt gactacaaga gcgcacaaca agcgcttgag gagatgaagc
     3181 tcgacctgat gaaagcccca aagaccgacg aggacaagca gaaggccgag gaactcagga
     3241 agcagatcaa caagctgggc gatcagctca aggagcttgc cgagaacgat aagaaactcc
     3301 aacaggaact ggccaagacg ggcatgaagc cggacgaaat cgcccgggct ctcgagcatc
     3361 tgaagaagca ggacctcgac gctctgaaag aacagctcaa gaagcagggg ttgtcggaga
     3421 aggacatcca gaagctggcg aaccagctca agaaatgcca aggcggttgc cagatggccg
     3481 gcaggctggg agacagcctg aagcaggcgg cccaaggcca gccgggacaa caaggttcgg
     3541 ggcagggatc cgaagccggg ttcaccgacg cggccgacca gttgtcggat atggaggcct
     3601 tgcagcagga gatgaaccag ctcacggcag cggccagtga tctgcagcag atgcaggatc
     3661 aactgtcgca ggccggacaa tgctcgggac agtgtgatgg gatggggttg ggcgagggcc
     3721 aaggccagtt gaaggagtcg cagagcggca tgggtccact tggtcaggga cggggcggca
     3781 ttgcgcctaa gactgagact ggtgccaaga ccacggctca gaaggctaaa gtcgagtcgc
     3841 accccggcgc gatcatttcc acccagtttg tccatggcga gcagattgcc ggacaggtga
     3901 ccgacgagtt tgttgaggcg gtgatctcca aggagcggga ggtctcggat gccatcaatc
     3961 gcgaggcaat cccgcgccag taccacaaga gtgtgagcaa gtacttcagc gacgccagag
     4021 agggcctgcc tgccgaccgg gtcaaggccg tcgagaacaa gttcgaaggc gaaaagccgg
     4081 ccggggaggc aaagtgagat gggtggtttc tcatttccgc gggcgcaggc gatttcgtgc
     4141 gccctggcgg cggccgcttg tgcattcctt tccgggtgcg accaatcaag cgacaagccg
     4201 gcagtcactc tctataccag cgtcgatgag cccttcgcgc gagaagtgat tgcagccttc
     4261 gagaagcaga gcggcattcg ggtggcgatc aagatcgaca gcgaggccgg gaagacaacc
     4321 gggctggttc gacgaatcga tgccgagcgc aaccgcccgc aggccgacgt tttctggtcc
     4381 agtgagctgt tcaacaccat caagatgggg cggcaaggcc tgctggccga gtatcgcccg
     4441 ccggccgagg ggattcccga tcgctacaag gacccggccg gccggtggac ggcctttggc
     4501 atgagagccc gcgtgttggc gttcaacacc tcgaaggtca agcccgagga gcttccgacc
     4561 cggtggcgcg agatcgccaa ttcccgctgg gcaggagtcc ttggcgttgc cgatccgcgt
     4621 ttcggcacca cccgggggca ctttgccgcc ttcctggccg catgggggga gccgacctat
     4681 gcggagttcg tcgagcagtt gagccagacg ctgggcgata agctgcagga cggtaatgcc
     4741 gccgctgctc gggcggtggc caggggcgaa ctgcacatct gtgccaccga taccgacgac
     4801 gtctatgttc gtcaggacgc caaggagccg atcgacatgg ttttccctga tatgggtgac
     4861 ggcggcacgc tgctgatccc caactcggtt gccatgatcg ccaatgctcc tcacccggat
     4921 gaagcaaaga gactgataga tttcctgacc tcagagcaga ccgaacgaat gctggcccgg
     4981 agtccatcgc gcaactaccc cgttcgtgaa tcgctgcgca aggaactgag catcactctc
     5041 ccgcccgagg cgaagctgac attcgagcag attgccgacg ctatggacaa agccatcgaa
     5101 ttggcgggca ggaagctgat caagtaggca ttgttttgtg ttttgacacc ttctaataaa
     5161 aaagtcttct aagctcgcac gactgtttcc cgacccgtgc ccgaggcaaa gcgcagaggg
     5221 gccccgttca gccaaggaca ggggctcggt ttccaacagc ggggccgatg ggagtggatg
     5281 ttgccatggg aagtcgcaag gctcgtgctc gcggatcccg cggctttgtg gtgaagcgcc
     5341 ttgatatcgg ccgcatgatc aagcaagccg ctcaatcgcc ggaggcgccg aaaacgtgga
     5401 gaagaacctc gacaacaggg gaaccgggaa acatcggccc gcagatgagg ccggacggac
     5461 ccggatggcc gggcgatcat cgtccggctc gcccggcgtc gcctcttatc ttctgccccc
     5521 cgccgctcct caaaaacgag ctgcgtcccc gtctcaacat tcggtcgaac tggaggtcag
     5581 ggtgagactt gccgtcaatc accgtgtcgg acaactgtgc ggcctcgtgc gcgagtatcc
     5641 caaggcgctc gatcccagag caatgtcggc ggcggttgaa atgcgcgggt tttcggcggt
     5701 tcaggccgca gtggtggttt tggttcttct cggacgtgat aagtgcaata ggctactt
//
LOCUS       DAOTPW010000306         5758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11835_length_5758_cov_5.214975, whole genome shotgun sequence.
ACCESSION   DAOTPW010000306 DAOTPW010000000
VERSION     DAOTPW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11835_length_5758_cov_5.214975"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="P5316_19650"
     CDS             <1..412
                     /locus_tag="P5316_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRR87231.1"
                     /translation="NGDIACFVAGVGSGGTLQGVGKFLREHKPDVRLVAVEPKNASAL
                     LGHEPGLHQIQGIGDGFIPAILDVKLIDEVIEVTDEDAIETTRQLGRDFGLLVGISSG
                     ANVWAARQLAKRVTGNIATVLPDRAERYFSTALL"
     gene            complement(616..1497)
                     /locus_tag="P5316_19655"
     CDS             complement(616..1497)
                     /locus_tag="P5316_19655"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRR87232.1"
                     /translation="MTKNLMAATLLAAAAAVTGCSEIRMGEPECDKRMPHWRLAMMSY
                     TFRQFTVFEAVDKTKALGLEYLEEFSWHKIGGPCGDAKFDETAPPEARQALKNKLNET
                     GVKVVGCYVQVIGKDEAATRRTFEFCKDLGIEYIVCEPDPGDLALVDKLAGQYKVYVA
                     IHNHWRDVNKPEYRNWNPDEVLKMVQNRSKWIGLCADTGHWARSGLDTVEAVRKCRGR
                     IVSLHLKDIEKPDPKAHDVVWGTGVANLSGVLAELAHQKFRGVFSIEYETNPADNIAE
                     VAQCIKFFNQVSNELAR"
     gene            complement(1717..2781)
                     /locus_tag="P5316_19660"
     CDS             complement(1717..2781)
                     /locus_tag="P5316_19660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRR87233.1"
                     /translation="MAELHDDNDDDVILNPELADRPLDVDSEEAQAVTIYIRRRLPGR
                     RLDKYLHGRFQRMSRTTIQRLIKQGVITVNRKPTKASYEPEAGDRIDLVIPPPEPYEI
                     IPEPIPLDIIYEDEVMLAINKPKGIIVHPSCGTQTGTIANGLAYYARQLSHGDDPFRP
                     GIVHRLDKNTTGIMLVAKTDEAHWRLAIQFERRLVRKTYLGVVEGNPELDSDVINAPL
                     AAHNVIKDRYIIPPSHHRWRSRLLKEAVTEYEIVERFRGYALAHLHPRTGRTHQLRVH
                     MSYIGHPMAGDTFYGGHHVSELSLTGQGSDEPITSQQALHAYRIRFKHPISEAQMELE
                     APPPPELAKLIQILREHRRL"
     gene            complement(2959..5079)
                     /locus_tag="P5316_19665"
     CDS             complement(2959..5079)
                     /locus_tag="P5316_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87234.1"
                     /translation="MYARPSQIIVLLGLLVPLCLAALCAAPGSGFTSGDMLADTSDAQ
                     DASQALTEAIKGGASTQEAVDSLVHTLRANPSIVVAVADEDGRTAWALFESGLECVYE
                     VIDEEETELTLPAVQATAASSLACAVAPVRPHGLATSARDSPFSIAQAGWTSYKLPAN
                     NRAVLANSLYVTRPLQDVRTPMKTMLEACGYDVQVVDADLTFFENMSQYSVIFIEAHG
                     GRRSSAETTWDSAANTFYNLVLKQNLPACGLIGAEAVLQTTTEVTQDLITQYKEDLEC
                     GRLKIRFPTVKRKGLPPVTYRFFGVTPNFVRRHNTGKFPDYALLCLSACRSFTPDGSP
                     WADLMSEKSYGSVTVGWDQRVHYGISARAMLNLFQLMTGSNEQFAIVQTDDSGGIKTI
                     YPLLDKYDPPVPPQTVGNALDALERRGFNLDPITTARLTAGGDTLYGFNQLMLAPAIE
                     TFETRGDGKVKMAARCADNAELEFSGGSVNIGTANNGEWEFALPGLRYGPMSLVMEGR
                     RCPPRDLLRWNPLVIKIHPASTTNAFGTCNFEVTYRLSARAIAAGNREGWSVWDPPDS
                     AFSANWDPDGSVVTWSISGNASDPDPMFGTSQCTWSGGNTRAFNEISISPGDTGGLDS
                     MDGKTAWLTVEPPFDLEYTASCSGAFAPDYACGIYGSIWIQVDLGADWTILPGSTTDL
                     LGMNVIEWQACTPTPDFDSARLPR"
     gene            complement(5383..>5758)
                     /locus_tag="P5316_19670"
     CDS             complement(5383..>5758)
                     /locus_tag="P5316_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118983.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRR87235.1"
                     /translation="QPAGKVVLGTVKGDLHDIGKNLVAMMLQGGGYEVIDLGTDVSAE
                     KFIAAAKDKGAGLIGMSALLTTTMPYMSQVVQAAKASGLKIKTMIGGAPVTQQYANEI
                     GADGYAADAASAVELARSLCAA"
BASE COUNT         1004 a         1649 c         1895 g         1210 t
ORIGIN      
        1 gaacggcgat atcgcgtgtt ttgtggccgg tgtcggcagc ggcgggacgc tacaaggggt
       61 gggcaagttc cttcgcgagc acaagccgga cgtgcggctg gtggcggttg agccgaagaa
      121 tgcttctgcc ttgctcggtc acgagccggg cctgcatcag atccagggca tcggtgacgg
      181 cttcatcccg gccattctgg acgtgaagct gatcgatgag gtgatcgagg ttaccgacga
      241 ggacgcgatc gagacgactc ggcagctcgg tcgtgatttc ggtttattgg tgggcatctc
      301 atccggtgcc aatgtctggg cggctcgaca actggccaaa cgggtcacag gcaacattgc
      361 cacagtactg ccagaccgtg cggagcgcta tttcagcacg gcgctactgt gagaagaagc
      421 tattagctgt cagctttcgg cgatcagctt cagatggttt gcccttgttg tccggctccc
      481 ggaatggaga cggagagacc ctcagcaaac ggagtcggcc ggccctttgt cgtctccgtt
      541 ggctcctgtc tggtctatcc ttgccgcgtt tttcggacca tctccccctt cccagccgtc
      601 aacgcgcctg tttggttacc tcgccagttc gtttgacacc tgattgaaga acttgatgca
      661 ctgggcgact tcggcgatgt tgtcggccgg gttggtctcg tactcgatgg agaagacgcc
      721 gcggaacttc tggtgggcca gttcggccag gacgccgctg agattggcga cgccggtccc
      781 ccagaccacg tcgtgggcct tgggatcggg tttctcgata tccttgaggt gcaaggagac
      841 gattcgcccc ctgcatttgc ggacggcctc gacggtgtcg agtcccgagc gggcccaatg
      901 gccggtgtcg gcgcagagcc cgatccactt gctgcggttc tggaccatct tgagcacctc
      961 gtcggggttc cagttccggt attcgggctt gttgacgtcc ctccaatggt tgtgaatggc
     1021 gacgtaaacc ttgtactggc cagccagctt gtcaaccaga gccaagtccc cggggtccgg
     1081 ctcgcagacg atgtactcaa tgcccaggtc cttgcagaat tcgaaggtcc gtcgggttgc
     1141 ggcctcatcc ttgccgatca cctggacgta gcagcccacg accttgacgc ccgtctcgtt
     1201 gagcttgttc ttgagagctt ggcgagcctc gggaggagcg gtttcgtcga actttgcatc
     1261 gccgcacggc ccgccgatct tgtgccacga aaactcctcg agatactcga gacccagcgc
     1321 cttggtcttg tcgacggcct cgaagacggt gaactgtcgg aaggtgtagc tcatcatcgc
     1381 cagccgccag tggggcattc ttttgtcaca ttctggttca cccattctga tctccgagca
     1441 gccggtcact gccgccgccg cggccagcaa tgttgccgcc atcagattct tggtcatcga
     1501 cttgtcctcc acatcgaagg ttgaacactc gtctcacccg gtcaagctgg aatataacgg
     1561 cgccgcttcc atccgcgcaa ggctgttcct gccggcagcg ccggagattc acgaccgtga
     1621 ggatacatga agggggcccg gcattccaga ttatcgggcc tgccggcggt ccgtgtatcc
     1681 ctggcacaca aggcggtttg gtgtcgcaac cgggtgctac aagcgacgat gttctcgcag
     1741 gatttggatc agcttggcca actcgggtgg cggcggcgct tccagctcca tctgagcctc
     1801 ggagatcggg tgcttgaagc gaatgcggta ggcgtgcagt gcctgctggc tggtgatggg
     1861 ctcgtcgctg ccctggccgg tgaggctgag ttcggacacg tggtgcccgc cgtagaaggt
     1921 gtcgccagcc atcgggtggc cgatgtagga catgtggacg cgcaactggt gcgtgcggcc
     1981 ggtcctcgga tgcagatggg ccagcgcata gcccctgaac cgttcgacga tttcgtactc
     2041 ggtgacggcc tccttgagca gcctggatcg ccaacggtga tgggagggtg ggatgatata
     2101 ccggtccttg atgacgttgt gggcggcgag gggagcattg atgacgtcgc tgtccagttc
     2161 ggggttgccc tcgaccacgc cgagataggt tttgcggacg aggcgtcgct caaactgaat
     2221 ggccagccgc caatgagctt cgtcggtctt ggccacgagc atgatgccgg tggtgttctt
     2281 gtccagtcga tgaacgatgc cgggacggaa ggggtcgtcg ccgtggctga gttgcctggc
     2341 atagtaggcc agtccgttgg cgatggtgcc ggtctgcgta ccacaggagg ggtgaacgat
     2401 gattcccttg ggcttgttga tcgccagcat gacctcgtct tcgtagatga tgtccagtgg
     2461 tatcggctcg gggatgattt catagggctc gggcgggggg atgaccagat cgatccggtc
     2521 gccggcctcg ggttcatagc tggccttggt cggtttgcgg ttgacggtga tgacgccctg
     2581 tttgatcaga cgctggatgg tcgttcgcga catccgctgg aaccggccgt ggagatattt
     2641 gtccagcctt cgtccgggga gccgccgccg gatgtagatg gtgaccgctt gagcctcctc
     2701 gctgtccaca tccagcggtc ggtcggccag ttcgggattg agaatcacgt cgtcgtcgtt
     2761 gtcatcgtgc aactcggcca aaggcggtca cctcgtgtcg aaccatcgtg tgtgttgggg
     2821 tactattctc ccctcgcacc atcataacag gctctggttg atattgataa agaccatacg
     2881 gcaggtctcg gtcgctgcgg cgacctcgat gttgccctac gatgtggtgc cgccggtttt
     2941 cacccatgtg gaagggaatc agcgcggcag gcgagcactg tcgaagtccg gggtcggggt
     3001 gcaggcctgc cactcgatca cattcattcc caagaggtcg gtggtcgagc cgggcaggat
     3061 ggtccaatcg gcccccaaat cgacttgaat ccagatcgag ccatagatgc cgcacgcata
     3121 atcaggcgcg aacgcaccgg aacaggaagc cgtgtactca aggtcgaatg gcggctcgac
     3181 ggtcaaccag gcggtctttc cgtccatcga atccagccca ccggtgtcgc ctggtgagat
     3241 ggagatctcg ttgaaggccc gtgtgttgcc gcccgaccac gtgcactgcg acgtgccgaa
     3301 catggggtcc gggtcgctgg cattgccgct gattgaccag gttaccaccg aaccgtccgg
     3361 atcccagttg gcgctgaacg cggagtcggg cgggtcccag acggaccacc cctcgcggtt
     3421 tccggcggcg atggcccggg cgctgagccg gtaggtgacc tcgaagttgc acgtgccgaa
     3481 agcgttggtg gtggaagccg ggtgtatctt gatgaccagc gggttccatc gaaggagatc
     3541 tcgcggcggg caccttcgcc cttccatcac cagggacatg gggccatagc gtaagccagg
     3601 cagcgcgaac tcccattcac cgttgttagc ggtcccgata ttgaccgagc caccggagaa
     3661 ctcgagctcg gcgttgtccg cgcagcgggc ggccattttg accttaccgt cgccgcgcgt
     3721 ttcgaaggtt tcaatggccg gcgcaagcat gagctggttg aagccgtaga gtgtatcccc
     3781 gccggcggtc agtcgggcgg tcgtaattgg gtcgaggttg aagcctctcc tctccagggc
     3841 gtccagggcg ttgccgaccg tctggggagg caccggcggg tcgtacttgt cgagcaaagg
     3901 atagatggtc ttgatgccgc ccgagtcgtc ggtctggaca atcgcgaact gctcgttgct
     3961 acccgtcatg agctgaaaca gattgagcat ggctcgcgcc gaaatgccat agtggacacg
     4021 ctggtcccag ccgacggtga ccgagccgta agacttctcg ctcatcagat cggcccacgg
     4081 gctgccgtcc ggcgtgaagg agcggcacgc gctgagacac aagagggcgt aatccgggaa
     4141 cttgcccgta ttatggcggc gcacgaagtt gggggtgaca ccgaagaagc gataggtcac
     4201 gggaggcagt ccctttcgtt tgacggtggg gaagcggatc ttgagccggc cgcactccaa
     4261 gtcttccttg tattgcgtga tgagatcctg agtcacctcg gtggtggtct ggagcacggc
     4321 ttcggccccg atcaggccgc atgcgggtag gttctgcttc aggacgaggt tgtagaaggt
     4381 attggcggcc gagtcccaag tcgtctcagc ggagctccgc cggccgccgt gggcctcaat
     4441 gaagatcacg ctgtattggc tcatgttctc gaagaatgtc aagtcggcgt cgacgacttg
     4501 tacgtcgtag ccgcacgctt cgagcatggt tttcattggc gtcctgacgt cctgtagggg
     4561 ccgcgtgaca tagagtgaat tggcgaggac ggccctgttg ttggccggca gcttgtacga
     4621 cgtccagcct gcttgagcga tggagaaggg cgagtctctt gccgaggtgg cgaggccgtg
     4681 aggtcggacc ggcgcgaccg cgcacgcgag gcttgacgcc gcagtcgcct ggaccgcagg
     4741 cagcgtgagc tcggtttctt cctcgtcgat cacctcgtag acgcattcaa gaccggattc
     4801 aaagagggcc caggcggtcc ggccgtcctc gtcggccacg gcgaccacga tactcgggtt
     4861 ggctctgagc gtatgcacca gggaatcgac cgcttcctga gtcgaagcac ctcctttgat
     4921 cgcctctgtg agtgcctgac ttgcgtcttg ggcgtcggag gtgtcagcga gcatgtcgcc
     4981 cgaagtgaag ccactgccag gggcagcgca cagggcggcc agacaaaggg gtacaagcag
     5041 gccaagcaga acaatgattt gggacggtct tgcgtacatg aaggtgatct ccatatcgaa
     5101 gatgcgcgtt tagccccgcc agccgacgtg cccctgggat tcgtgacggg gcatggcgca
     5161 ggaccgtaac cccccagtat acagcagtcc tgccccggtt gaacctgttc tgatacgtga
     5221 tttcccggat ggtcttggtt gatcatccgg tcaatgcgtt tgcggttgaa ggagaaaaga
     5281 ctgcaagaga acggcccgcc agtagagggg caaccggggt tggtcctata aaaaccgcac
     5341 ttttgtccgg cagcgccgga aacgtcgcct gtcccggctg gctcaggccg cgcacaggct
     5401 gcgagccagt tcgacggcgg aggcagcgtc ggcggcatag ccgtcggcgc cgatctcatt
     5461 ggcatactgc tgggtgacgg gggcgccgcc gatcatggtc ttgatcttga ggccggaggc
     5521 tttggctgct tgaacaacct gggacatgta aggcatggta gtggtcagaa gggcactcat
     5581 gccgatgagg ccggcgcctt tgtctttggc cgcggcgatg aacttctcgg ccgagacatc
     5641 ggtgcccaga tcgatgacct cgtacccgcc gccctggagc atcatggcga cgaggttctt
     5701 gccaatgtcg tgcaggtcgc ccttgacggt gccgaggacc actttgcccg ccggctgg
//
LOCUS       DAOTPW010000307         5724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11902_length_5724_cov_4.991180, whole genome shotgun sequence.
ACCESSION   DAOTPW010000307 DAOTPW010000000
VERSION     DAOTPW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11902_length_5724_cov_4.991180"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            565..1626
                     /locus_tag="P5316_19675"
     CDS             565..1626
                     /locus_tag="P5316_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRR87236.1"
                     /translation="MRQDSIWPVVAITTVLTSLSLPAAAAADADSARHGELWVMYEGP
                     DGPGKGKHVVLVSGDEEYRSEEALTQLGKILARHHGFKCTVLFAVDPKSGMIDPTVPD
                     NIPGLEALERADLMIIATRFRDLPDEQMKYIDAYVSSGKPIIGMRTATHAFKIAKGKT
                     YERYSFDSKVQGWDGGFGRRILGETWISHHGHHGKQSSRGVIAPGMKDHPIVRGCEDI
                     WGPTDVYTVRLPLPGDSEPVVLGQVLEGMKPTDKPVSGKQNDPMMPIAWTKTYQGAKG
                     EAGRVFTTTMGAATDLENEGLRRLLVNAAYWCLGLEEKIPPRANVEIVGEYKPSPFGF
                     AGHKKGLKPADYRMKPADN"
     gene            complement(1974..2597)
                     /locus_tag="P5316_19680"
     CDS             complement(1974..2597)
                     /locus_tag="P5316_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRR87237.1"
                     /translation="MPRLLYVEASPRKERSTSIRVAGEFLDTYRDAHPRDVVDVVDLW
                     QEELPPLDGEAINAKYAILHGQSHSTAQAAAWAKITAVADRFTSADKYLFSLPMWNFG
                     IPYRLKHYIDVITQPGLTFTFSPAEGYKGMVTGKAAAVIYARGGEYQGDGASWDFQKP
                     YFELWLQFIGFTDVRGIVVEPTLGNRDATEKSKAAGCELARRTAIHF"
     gene            3381..4061
                     /locus_tag="P5316_19685"
     CDS             3381..4061
                     /locus_tag="P5316_19685"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR87238.1"
                     /translation="MRHTLVVILGLLVMTSGCEKGGGVAPAPVEKMPTAIQLFNGRDL
                     TGWTCDLSKEGVKMEDVWSVADGVLHCKGEPGGVLRTLDEYGDYVLSLEWRWPPGTQG
                     GNNGVLVHTSTPRTLGIWPKSIEVQLEKGNAGDFWVIGTELDVENEQARKQGRRHLNL
                     TDGSEKPLGEWNHMEITCKGDEVIVRVNGELVNHATNCSVTRGAICLQSEGAPIEYRN
                     IILTPLPR"
     gene            4226..>5724
                     /locus_tag="P5316_19690"
     CDS             4226..>5724
                     /locus_tag="P5316_19690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRR87239.1"
                     /translation="MSGKNRIPDRVAGEPRWRPEPSRAWLALPPVVEYHRDMAIVSFQ
                     DVHFGYEGQVLFDGLDWTIHRGEKVGLVGPNGSGKTTLFRLILGQLSPAVGTVARNRD
                     LRIAYLPQEPQLDSSNTLLAEVATTFDHLRGLDARVAEVAQRLADHHDTDQYEDLLAE
                     YEELQHRLEAAGGYKYETLVKMVLGGLGFTPASYDLPISALSGGQKCRAALAQMLLQE
                     ADLLLLDEPTNHLDIEATRFLENFLAQYDGAAVIVSHDRYLLDRVVTKIADLDRKRIT
                     VYPCAYSDYAESKLTRQLTAEREYARQQEWLQHQREYAERVKADKSRSRQASGRLRHV
                     ERLERDGSVVERPSHMQRRMAIRLTPGRRAGEMVLRCTGVRKAYGQVVLFDDFNLEIY
                     RGEKIGIIGPNGVGKSTLLKMAMRQIAPDAGEVRLFENLEVGYYDQEHAGLNMDHRVI
                     DVIVERPTGPLEARIRSFLARFLFIGDDVYKRVGDLSGGEQSRVMLARLV"
BASE COUNT         1094 a         1700 c         1875 g         1055 t
ORIGIN      
        1 gagagtactc ggagcgatgc cccggagcaa tttacacagg agttgacact gcctctcgag
       61 gccaggatag ttgccattgg agatgccgca cgtcggctcg gttccagcga acggcccgcg
      121 cccgctggcc ccgtcggaca tcccgggatt ccggagagta tcctgccttc gcgctcccag
      181 acttaagaca tattacgcct gcggatccgt ctgtatcaag cacattttgg gtctcttcaa
      241 gggaacagat aaagaaaagg ttcagccgac agaatgaagc tggtgcatat cgggccggtc
      301 tctttaagct ctccggcgtg ggcagttcgc ccatcttggg ctgcggcgct gcgcgtggga
      361 cgaagaacag ggcacaggat ctgttcgttc ggttgactgt tgaacgcaag tcccgaacag
      421 tagacgagcg ttttttgttg tccttgttgg ttcgacagag attggcggcc gtcttatact
      481 cgcgggcctc tgaggtatgg cagtcagtgc gggggaattg acgcccagaa cgtccgcctc
      541 cgtccgcgaa aggagatgcc gggtatgagg caagactcaa tctggcctgt tgtcgcgatc
      601 accacggttc tcacgtcgct cagcttgccc gcggccgcgg ctgcggatgc cgactctgcg
      661 cgtcacggtg agctgtgggt tatgtatgaa ggccctgacg gtccgggaaa gggcaagcac
      721 gttgttctgg tctcgggcga tgaggaatat cgctcagagg aagccctcac tcaactcggc
      781 aagatccttg ccaggcatca cggattcaaa tgcaccgtgc tcttcgcggt ggatccgaaa
      841 agcggtatga tcgatcccac ggttccggac aacatcccgg gccttgaggc gctcgaaaga
      901 gccgacctca tgatcatcgc cacgcgcttc cgtgatctgc ccgacgagca gatgaagtac
      961 atcgacgcgt acgtgtcatc gggcaagccg atcattggta tgcggacggc gactcacgcg
     1021 ttcaagatcg ccaagggcaa gacctacgag cgctacagct ttgacagcaa ggtgcagggc
     1081 tgggatggcg gtttcggccg tcgcattctc ggcgaaacct ggatcagtca ccacggtcat
     1141 cacggtaagc aaagttctcg cggcgtcatc gcgccgggga tgaaggatca cccgatcgtc
     1201 cgcggctgcg aggatatctg gggacctacc gatgtctaca ccgtgcgttt gccgctgccc
     1261 ggcgacagcg agcccgtggt cctgggccag gttctggagg ggatgaaacc cacggacaag
     1321 ccggtatcgg gcaaacagaa tgatccgatg atgcccatcg cctggaccaa gacctatcaa
     1381 ggcgccaagg gggaggcggg ccgcgtgttc acaaccacga tgggagccgc aaccgatctg
     1441 gagaacgagg gtttgcgtcg actgctggtc aacgccgcct actggtgcct gggtctggag
     1501 gagaagatcc cgccgagggc caacgtcgag atcgtcggcg aatacaagcc gtcaccgttc
     1561 ggttttgcag gtcacaagaa gggcctcaag ccggccgatt acaggatgaa gccggctgac
     1621 aattagcgca acggcgcgag gttgccgtgt tccatggagg gcagcccccg ggccggtcgt
     1681 tggtccatcc cctgcgacca actgggcctg cctcggatta ggtgtcccgc cgcacctgcc
     1741 gggatcaccg tgccaggtcg cgtttcaaga aagcgcccat atgtcatcgt ttccttgtag
     1801 cgcctctggc gtcaagcccg tccttctgat gttagccggg gcggatggag tggccgggtg
     1861 atgaagtctg atgcagagtt cgtgagggcc gtcctggctg gcgatggcga ggccttttcc
     1921 gacagggacc gtgacgggct tcctctcacg agcccgcgac cgtctgcccc ggttcagaaa
     1981 tgaatggccg tccggcgggc cagctcgcac cccgcggcct tggacttctc ggtggcgtcg
     2041 cggttaccca gtgtgggttc gaccacgatg ccccggacgt cggtgaagcc gatgaattgc
     2101 aaccacaact cgaagtaagg tttctggaaa tcccacgatg cgccgtcgcc ttggtactcg
     2161 ccgccgcggg catagatcac cgcggcggcc ttgccggtga ccattccttt gtagccttcg
     2221 gcgggcgaga aggtgaaggt gagtccgggc tgggtgatca cgtcgatgta gtgcttgagc
     2281 ctgtaaggga ttccgaaatt ccacatgggg aggctgaaga ggtatttgtc ggccgaggtg
     2341 aaacgatcag cgacggctgt gattttggcc catgcggccg cctgagcggt cgagtgcgac
     2401 tgaccatgaa ggatggcgta cttggcgttg atggcctcac cgtcaagcgg cggaagctcc
     2461 tcctgccaca ggtccactac gtcaacgacg tcgcgcggat gagcatcgcg gtacgtatca
     2521 aggaattccc cggcgacgcg aatcgaagtc gagcgttcct tgcgaggcga agcttcaaca
     2581 tagagcagtc tcggcatgac gcggcctcct gcggcggact ggctgtccct taatcacgac
     2641 aagtgactga aaggccggtg ccgcgcaaca cagtgtagca ctcgtgccgc ctcgcggcca
     2701 gcgggccggc gatcgggcgg cggcgggtgc gtcacgtgtt tggcgggttg tgttagacgg
     2761 acgaggctga gtaaccgacc cccttcggaa ggtcttttcg ccgaaggtcg gcagatcacg
     2821 ccgaaggtgg acgggctgat gggccgggca ttgtacggcc ggtggttcgc gacaacaccc
     2881 gtcgtccctt tccacaattc cagcccgttt caacgaactt ccagaaaagc ctgttgaaac
     2941 ggcctcactg tgaaaagaca gggagagaga cacgggtgcc cgatcaagaa ccagccgtca
     3001 aacggctggc tattggccct tcgggaaaag ccgctgaagc ggcttgtttc cggcttgcat
     3061 ctgccgctac cccaaaaaac cacagcgaag gcggccgatt ccgtcacgga cccggcggca
     3121 gcgaccctct tgccgtcgcg ccggcaccgc tttcggtagg ggcggcatgc gccggcgggc
     3181 aagcccctcg acacaggctc gggatgcaag cggcaaatgg catccgccgc gcgcacacga
     3241 gggcgaaagg ctcagcagcg cagatgctgt gggtggcggt cccggcgagc ggaacgtaga
     3301 atgtcgcggc aagatccggc ctcgtggccg ggcaaggtgt ggcggcggac gtcggggcat
     3361 tgtccgacag gagcgatacc atgcggcata cactggtagt gattttgggc ttgctggtca
     3421 tgacttccgg gtgcgagaag ggcggtggcg tggcccctgc tcccgtggag aagatgccca
     3481 cggccatcca gctcttcaat ggccgtgacc tgaccggctg gacctgcgat ctgagcaagg
     3541 agggcgtgaa gatggaggat gtctggtcgg tcgccgacgg cgtccttcac tgcaaaggag
     3601 agcccggcgg tgtgctgcgg acattggatg aatacggtga ttacgtgctc agcctggagt
     3661 ggcgatggcc gcctggcact cagggaggca acaacggcgt tctcgttcac acctcgaccc
     3721 cgcgcaccct cggcatctgg cccaagtcga tcgaggttca gttggaaaaa ggcaatgcgg
     3781 gcgatttctg ggtcatcggc accgagctgg acgtcgagaa cgagcaggcc cgcaagcagg
     3841 gccgccggca cctcaacctg accgacggct cagaaaagcc gctgggcgag tggaatcaca
     3901 tggaaattac ctgtaagggc gacgaggtga tcgtccgggt caacggggag ctggtcaatc
     3961 atgccaccaa ctgcagcgta acccgcgggg ccatctgcct gcaatccgag ggcgctccga
     4021 tcgaataccg caacatcatc ctgacgccgc tgccgaggtg agcctggggc gctcagacct
     4081 cgttgcatca agcggcgacg ccgctgacct caaatactga ccgggctctc tgtcgcaagc
     4141 atgccgatcc tcattcacgc ccggcgcgtg ccgattcgcc acgcggcagc cgcctgcaaa
     4201 gtccgtgcct gttggagccg ggatcttgag cggcaagaac cgtatcccgg atcgcgtcgc
     4261 aggcgagcca cgctggcggc ctgaaccctc tcgtgcgtgg ttggctttgc ctcccgtcgt
     4321 cgaatatcat cgcgacatgg cgattgtgtc gttccaggat gttcatttcg gttatgaggg
     4381 gcaagtgctc ttcgacggtc tggactggac gatccaccgg ggcgaaaagg tgggtcttgt
     4441 cggacctaac ggctcaggca agacaacgtt gttccggctg attctcggcc agttgagtcc
     4501 ggccgtcggc accgtcgcca ggaatcggga tttgcggatc gcctacctgc ctcaggagcc
     4561 ccagctcgat tcgtccaaca cgctgttggc cgaggtcgcg accaccttcg accatctgcg
     4621 ggggctcgat gcccgggtgg cggaggtggc ccagcggctg gccgatcatc atgacaccga
     4681 ccagtacgaa gatctcctcg ccgagtacga ggagcttcag catcggctgg aagcggccgg
     4741 gggctacaag tacgagacgc tggtcaagat ggtcctaggt gggctgggat tcactccggc
     4801 gtcctacgac ctgccgatat ccgccctgtc cggcgggcag aaatgccggg cggccctggc
     4861 ccagatgctc ttgcaggagg ccgacctgtt gctgctcgac gagccgacca accaccttga
     4921 catcgaggcg acgcgcttcc tggagaactt cctggcacaa tacgacggag cggccgtcat
     4981 cgtatcccac gaccgctacc tgctcgaccg ggtcgtgacc aagatcgctg acctggatcg
     5041 taagcggatc accgtttacc cgtgcgcgta ttccgactac gccgagagca aactgacgcg
     5101 ccagttgacc gccgagcggg aatacgccag acagcaggaa tggcttcagc atcagcgtga
     5161 gtatgccgag cgggtcaagg ccgacaagtc gcgttcacgg caggcgtccg gccggctcag
     5221 gcacgtcgag cgcctggagc gcgacggctc cgtggtcgag aggccctcgc acatgcagcg
     5281 tcgcatggcc atccggctca cccctggccg ccgggccggc gaaatggtcc tgcgatgcac
     5341 cggcgttcgc aaagcgtacg gccaggtcgt cttgttcgac gatttcaacc tggagatcta
     5401 ccgcggcgag aagatcggca tcatcggacc gaacggcgtc ggcaagagta cgctgctgaa
     5461 gatggctatg cggcagatcg ctccggacgc cggcgaggtg cggctgttcg agaacctgga
     5521 ggtcggctat tacgaccagg agcatgccgg gttgaacatg gaccaccgcg tgatcgacgt
     5581 gatcgtcgag cgacccacgg ggcccctgga ggcacgcatt cgctctttcc tcgcgaggtt
     5641 cttgtttatc ggcgacgacg tgtacaagcg ggtgggcgat ttgtccggag gcgagcagag
     5701 ccgggtgatg ctggcccgat tggt
//
LOCUS       DAOTPW010000308         5711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_11932_length_5711_cov_4.932461, whole genome shotgun sequence.
ACCESSION   DAOTPW010000308 DAOTPW010000000
VERSION     DAOTPW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11932_length_5711_cov_4.932461"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="P5316_19695"
     CDS             <1..183
                     /locus_tag="P5316_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87240.1"
                     /translation="HSPARALDDPRALGRTNIGFLDYHVESRMYPARTWADELFTEFG
                     QPTSGKNLKAFVLGNY"
     gene            351..4238
                     /locus_tag="P5316_19700"
     CDS             351..4238
                     /locus_tag="P5316_19700"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRR87241.1"
                     /translation="MTGPQFRLNLSVAAVLLWLCAGVVYANVYPSGVTVDASALNDTC
                     GTLKVTYVLNENADGDGVNPGVKIEVLDNTLTAVRTVSIARQKKGTHVFDWDGRKDDG
                     TRAPNGSYTVKITAADFGYTVWTQISVDQTSTSFYVPVGVSVNRNPGSPHYGSVYVSN
                     ATSGETAYGRNNPEGVYRLKADMTEINSGTAGVTWGGASGPWKSAIGTDDRVYVTDLS
                     NDLVFDVAPDLSSAVQLIDASNRTANQWVGAVVVEGTQAAGNRKIYLANVNYNDTARK
                     GLIQYTLGANPTVTPGDTGVQYIGPTYYSFYPYDYVRDSNGDWYGTQYRYDPTQAEAI
                     AKFLDGTPPINTAFWKTPMVSPYNGAYCLDIYEKRGWVAYGNYYDGWVHIFNTADGSY
                     VGGFDAGSRMRDIAFDAAGNIYTVDNVTEWLRAWSPPDGPNSMDYTTVAVSLAKSDTG
                     GPSITSQPDDVIFCPNGTASLSVVATGSNPAELAYQWKKDGVALTDGTQADGVIISGA
                     TTANLTMSNVPASYAGSVMTVVVCDQVGPDTGVVISDPAILKVGIFFIQHPASQMICQ
                     NTNVSFSVGVEGKGAISYQWQKDAAGSPVEWVDIDGETSDTLSLTNVPSTESGARFRC
                     AVRDECNPAPADPVYSAIATLTVRVGPTITHVGYGGTIPPGANHKLLCDITGVVGNAS
                     FQWKKDGIPIDGATNSYYWIINASCGVHSGLYSCAVTDDCGTTERSTDITGAPCTIIV
                     GAASEVCGNGLDDDCDGLTDCDDSDCDNDPNCLPPCPVPFADSDEDGDVDHVDFAAFQ
                     LCYTGNTTNLFDPQMCRCFDWDGDLDVDADDHSKFEDCASGPGVVAAKDCAPFPTGDV
                     VINEISYDMFTATGGADTDDDEFVELYNRGTEPANIAGWLLRASTGSNIIIPTTGGGP
                     TWLAPGDYYVIGSGNFFPNTDLIISTTDIWGNDETAIELLDRNQTVKDTVIYERYLAG
                     GPSVPSPEGGVWGSFGTTAFAFQSLARYLDGWDTNNNGYDFGLRPRTPGANNLTGGFD
                     PPIGSVMTAYTVPNVDALTPNTTVPGLLGSFRNATVIDPAVDTTANPNTINTNVIPAS
                     PQGGNAIIVADLPGGNMVASDNVFQGGAGYDMWVYLDTNPYGVGVTGGESTTYGIVGT
                     TDYLYSFPNPDSNPGFGGDVVTANGNTGVGWVYQKETDTNNVNYLMSLSLCDFGPGGD
                     SSGPPGSATPADWKVYATINLAGQPSGWHRLKLSYNATTGAVHAEFDAQTFDFTTATG
                     LVGNFYVGYRESLPNTPENDAKLRPATFDSVAP"
     gene            complement(4461..>5711)
                     /locus_tag="P5316_19705"
     CDS             complement(4461..>5711)
                     /locus_tag="P5316_19705"
                     /inference="COORDINATES: protein motif:HMM:NF018606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87242.1"
                     /translation="AAWDLATDQPVGEGRTNGIAGKHELKSSYPFYELMWEIDKQATR
                     EFIESFWASHILRWDILDMNRHGAYLPIPDKMWEQGYVGGPVPFAGQGLTFFLTGSDL
                     FCAAALLYVLDGGDQRPLVWAKRMAQRYADVRHPVTGLGADNYSIIEPDRMTRQFGPE
                     FGDRFTEATVTSLYGNRYNRSAICQLKLSERLGPAGEEFKRSAVEDLAAYAKHAYDAG
                     DNSFWATLIDGTKLTPADRKRDGYVEVRWLEKRPADGLHFWAYALAYKLSREPLMWQT
                     ARSIGRGIGLGDIGATPDTRPGPVRQASCTDVHAIFGLLELCAVTGRDEYLALASKVG
                     DNVLAKEFHKGFFVADRDHVFCSFDTVTPLALLYLHVAGHKSPVRLPLYVAGRGYFHC
                     PYEGAGRTYDHRVIYTMVRTSQPQ"
BASE COUNT         1158 a         1728 c         1721 g         1104 t
ORIGIN      
        1 cacagcccgg ccagggctct ggacgacccg agggcactgg gccgcacgaa tatcggtttc
       61 ttggactatc acgtcgagag ccggatgtat ccggccagga cttgggccga cgagttgttc
      121 accgaattcg gccagcccac atcgggcaag aatctgaagg cgtttgtgtt ggggaattat
      181 tgatccacgg gcggaggaat gatccagggg tcgccaatca gcggcccatg aaagaagcgc
      241 caagcaagac tcacatctat catgctgctt gctctgtgag ttgtacggcg tcgtggattc
      301 cgagcagatt gttcggtagg tatgttatcc ctaaggaaag gagccaaatc atgacagggc
      361 ctcaattcag gctgaacttg agtgtggcgg cggttctgct ctggctgtgc gccggggttg
      421 tatacgccaa tgtttatcca tcgggcgtga cggtcgacgc ctccgcgttg aacgacacat
      481 gcggaaccct caaggttacg tatgttctca atgaaaacgc cgatggcgat ggggtgaacc
      541 cgggcgtcaa gatcgaggtg ctggataata ccttgactgc cgtccggacc gtcagtatcg
      601 cccgccagaa gaagggcaca catgtgttcg attgggacgg ccgcaaggac gacggtaccc
      661 gtgcccccaa cggcagctat accgtgaaga tcaccgccgc agacttcgga tacaccgtgt
      721 ggacccagat cagcgtcgac cagacatcaa cgtcgttcta tgtacccgtc ggcgtatcgg
      781 tgaaccggaa tccgggcagc ccgcattatg ggagtgtgta cgtctcgaat gcgacgtcgg
      841 gggagaccgc atacggacga aacaatcccg agggggtcta caggctcaag gccgatatga
      901 ccgaaatcaa ctcgggcacc gcaggcgtga cttggggcgg ggcctccggc ccgtggaaga
      961 gcgccatcgg caccgatgat cgggtgtatg tcaccgattt gtccaacgac ctggtcttcg
     1021 atgttgctcc ggatctgtca tcggcggtcc agttgatcga cgccagcaac cgaaccgcca
     1081 accaatgggt gggtgccgtt gtcgttgaag gcacccaggc ggcgggcaac cgcaagatct
     1141 acttggctaa cgtcaattac aatgacacgg ccaggaaagg cctgatacag tataccttgg
     1201 gtgccaaccc gacggttacg ccgggcgaca cgggcgtgca gtacatcggc ccgacctact
     1261 attcgtttta tccctatgat tatgtcaggg acagcaacgg cgactggtac gggacacagt
     1321 atcggtatga ccccacgcag gcggaggcta tcgccaagtt cctggatggc acgccgccga
     1381 tcaacaccgc tttctggaag acaccgatgg tgtcaccata taacggcgcg tattgcctcg
     1441 atatctacga gaaacggggc tgggtagctt acggcaacta ctatgacggt tgggtacata
     1501 tcttcaacac ggccgatggt agctatgttg gtggcttcga tgcgggcagt cgcatgcgcg
     1561 acatcgcatt cgatgccgcg ggcaacatct acacggtcga caacgtgacc gagtggttaa
     1621 gagcttggtc cccgccggac ggtccgaatt cgatggacta caccacggtt gccgtctcgt
     1681 tggcgaaatc tgacaccggt ggcccaagca tcaccagtca gcctgacgat gtgatcttct
     1741 gtccgaacgg gaccgctagt ctcagcgtag tggccactgg ctctaacccg gccgagctgg
     1801 cttaccagtg gaaaaaggac ggcgtggccc tgaccgacgg tacccaggca gatggtgtca
     1861 tcatttccgg ggctaccacc gccaatctga caatgagcaa cgtcccggcc tcatacgcgg
     1921 gctcagtaat gaccgtcgtg gtctgcgatc aggttggacc ggacacgggg gtcgttatta
     1981 gcgacccagc catcctgaaa gtgggcattt tcttcatcca gcacccggcg agccagatga
     2041 tttgccagaa taccaatgtg agctttagcg ttggtgtgga gggcaagggt gcgatcagct
     2101 accagtggca gaaggatgcg gcgggcagcc cggtcgagtg ggtggacatt gatggcgaga
     2161 cttccgatac gctgtcgctg accaacgtgc caagcactga aagcggcgcg agattccgct
     2221 gtgccgtgcg ggatgagtgc aatcctgcgc cggccgatcc tgtgtacagc gcaatcgcaa
     2281 ctctgacggt tcgggtcggt ccgacgatca cgcatgttgg atacggtggg acaataccgc
     2341 ctggtgccaa ccacaagttg ctctgtgata tcaccggggt ggtcggcaac gcgtctttcc
     2401 agtggaagaa ggacggtatc ccgatcgacg gagccaccaa cagctattac tggatcataa
     2461 acgccagctg tggcgttcac agtggtttgt attcctgtgc agtcaccgac gactgtggaa
     2521 cgaccgaacg cagcaccgac attaccggcg ctccttgtac aatcatcgta ggggcggctt
     2581 ccgaggtctg cggcaacggg ctggacgacg actgcgacgg cctgacggat tgcgacgact
     2641 ccgactgcga caatgatccc aactgcttgc cgccgtgccc cgtgcctttc gctgactcgg
     2701 atgaagacgg cgacgtggat cacgtggact tcgccgcctt ccagctctgc tacaccggca
     2761 acaccacaaa cctttttgac ccgcaaatgt gccgttgctt cgactgggac ggcgacctgg
     2821 atgtcgacgc cgacgatcac agcaagttcg aggattgcgc cagcgggccc ggggtggttg
     2881 ccgccaagga ttgcgccccg ttccccaccg gcgacgtggt tatcaatgag atctcgtatg
     2941 acatgttcac cgcaacgggc ggcgcggata cggatgacga tgagtttgtg gagttgtaca
     3001 accggggcac cgagcccgct aatatcgccg gctggttgct ccgagcctcg accggcagca
     3061 acatcatcat ccccaccaca ggcggcggac cgacttggct cgccccgggc gactactacg
     3121 tcatcggctc gggcaatttc ttccccaata ccgatctgat catttccacg accgatatct
     3181 gggggaacga cgagacggcg atcgagctgc tcgaccgcaa tcaaaccgta aaggacaccg
     3241 tgatttacga gcgctatctg gcgggtgggc cctccgtgcc ttcgccggaa ggcggggttt
     3301 ggggttcgtt tgggaccacc gcgtttgcct tccagtcgtt ggcccgatac ttggacggtt
     3361 gggataccaa caacaacggc tatgatttcg gcctgcgacc aagaacgccg ggggccaata
     3421 acctgacggg cggcttcgat ccacccatag gttccgtcat gacggcctac actgtgccca
     3481 acgtcgacgc cctcacgccg aacacgacgg tgcctgggct gcttggttcg ttcaggaacg
     3541 ccaccgtcat cgacccggcg gtcgacacga cggcaaatcc caataccatt aacaccaacg
     3601 taatcccggc atcgccgcag ggcggcaatg cgattatcgt ggcagacctg cccggcggga
     3661 acatggttgc ctcggacaac gtcttccagg gaggagccgg ctatgacatg tgggtctacc
     3721 tggacaccaa tccctatgga gttggcgtga cgggcggtga aagcaccacc tacggtattg
     3781 tgggcaccac cgactacctc tacagcttcc cgaaccccga cagcaatccc ggtttcggcg
     3841 gcgatgtggt tacggccaat ggcaacacag gcgtcggctg ggtctaccag aaggagaccg
     3901 ataccaacaa cgtcaattat ctgatgagcc tgagcctgtg cgacttcggt cctggcggcg
     3961 atagcagcgg gcctccgggg tcggcgacgc ctgcggattg gaaggtgtat gcaaccatca
     4021 atctggccgg ccagccctcc ggctggcatc ggctcaagct gagctataac gcgacgacgg
     4081 gcgccgtcca tgcggaattc gacgcccaga cgttcgattt caccacggcg acgggcttgg
     4141 ttggcaactt ctacgtcggg tatcgtgaga gcctcccgaa cactccggag aacgatgcca
     4201 agctgcgacc ggcaactttc gactcggttg cgccgtaagc agccgggtct gacaatccaa
     4261 ggcccccggc tgcgttcttg tgccaacggt cttggcctca ccagggcctg accgtgatct
     4321 caagagttcc ggcagcgacc aaagggcgag gctcctcccg caaggggagc ctcgcctgtt
     4381 ttcttgctgc ccgaggatct gtggtgccga cgacgccctc gtcggcacgt cgcgagccgg
     4441 catcttctgg cttgcccggc tcattgcggc tgcgaggttc gaaccatcgt gtaaatcacc
     4501 cggtgatcat aagtccgtcc agccccctca tatgggcagt gaaagtagcc tctgccggcc
     4561 acgtacaggg gcagccgtac cggggactta tgtccggcaa catgcagata caaaagagcc
     4621 agcggcgtca ccgtgtcgaa actacagaag acatgatctc ggtcggcgac aaagaagccc
     4681 ttgtggaact ccttggccaa gacgttgtct ccgaccttgc tcgccagggc gaggtactcg
     4741 tctcgcccgg tgaccgcaca cagctccagg agaccgaaga tggcatgaac atccgtgcaa
     4801 ctggcttgcc gaacagggcc cggccttgtg tcaggcgttg ccccgatatc gccaagaccg
     4861 atgcccctgc caatgctcct ggccgtctgc cacatcagtg gctcgcggct cagtttgtag
     4921 gccagggcat aggcccagaa gtgcaggccg tccgcaggcc gcttctctaa ccagcgaact
     4981 tcgacatagc cgtcccgttt ccggtcggcc ggggtcagct tcgtgccgtc gatgagtgtt
     5041 gcccagaagc tgttgtcgcc cgcgtcgtag gcgtgcttgg cgtaagccgc gaggtcctca
     5101 accgccgatc gtttgaattc ctcgcccgcc ggtcccagcc gctcggagag cttcaactgg
     5161 cagatcgcgg accggttata ccggttcccg tacaggcttg taaccgtggc ctcggtgaag
     5221 cggtcgccga actccggccc aaactgccgc gtcatgcgat cgggctcaat gatgctgtag
     5281 ttgtccgcgc caaggccggt gaccggatgc cgaacatctg cgtagcgttg agccatccgc
     5341 ttggcccaga ccagcggccg ctggtccccc ccgtcaagca catacagcag ggcggcggca
     5401 cagaagagat cgctcccggt gagaaagaaa gtcagcccct ggcccgcaaa gggcaccggc
     5461 ccgccgacgt acccctgttc ccacatcttg tccgggatcg gcaaataggc tccgtgcctg
     5521 ttcatgtcca gaatgtccca tcgcaggatg tggctggccc agaagctctc gatgaactcg
     5581 cgcgtggcct gcttgtcaat ctcccacatc aactcgtaga aggggtaact gctcttcaat
     5641 tcgtgcttgc cggcgatgcc gttggtgcgg ccttcgccca ccggctggtc ggtcgccagg
     5701 tcccacgccg c
//
LOCUS       DAOTPW010000309         5681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12004_length_5681_cov_5.265197, whole genome shotgun sequence.
ACCESSION   DAOTPW010000309 DAOTPW010000000
VERSION     DAOTPW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12004_length_5681_cov_5.265197"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="P5316_19710"
     CDS             complement(<1..963)
                     /locus_tag="P5316_19710"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRR87243.1"
                     /translation="MRSRVFNCPWSRRLAYCGVAVMAAATAGEAYGRANIRDAFFSAY
                     PVAVGSTLDTVPSRPGHCGVCHYDFTGGGTRNPYGVRLGEVLPNFANNPNGRRQAVQS
                     IGNEDPDGDGFTSVVEITAVNQFSNTPTFPGLTPANVSNVSNVTVSEIQTHLVPTAGN
                     DTTPPTVQVLSPNGGDTLTGNAAAIVLWTATDASGIAAVDIYLSDDNGATFKPIAKGL
                     SNSGSHTFFVPNRPSTAAFVRVVAIDNAFNSGQDESDAPFTVERAPGGIAPTTLRDFD
                     MPGSQPFEAGTLNDPAACEVCHGGYDANVEPHFNWQGSMMAHASL"
     gene            1471..2289
                     /locus_tag="P5316_19715"
     CDS             1471..2289
                     /locus_tag="P5316_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR87244.1"
                     /translation="MSNLFDVKGKVVAITGGGGVLCGGIAEYLAACGTKVAVMDLKLD
                     AAEEVVRRIVAAGGIATAINANVLQRDSLEAAHKQCVETLGSVDVLINGAGGNHPAAT
                     TGPDKKFFDLPIDAFQKVMDLNIIGTILPSMVFGRAMAERGEGVIVNIASMNAFKPLT
                     RIPAYSAAKSGVKNFTEWLAVHMAQEYSPKIRVNGVAPGFFLTHQNRFLLMDQATGNL
                     TQRGQQIIAHTPMNRFGDPQDLYGTIIWLISPASQFVTGITVPVDGGYSAFGGV"
     gene            complement(2381..2869)
                     /locus_tag="P5316_19720"
     CDS             complement(2381..2869)
                     /locus_tag="P5316_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87245.1"
                     /translation="MPSSQYPSRANRRRLAGSRLASAGLLAAVCMGLMCQGDGGSDRP
                     LVDDQLSSRIDAASDALTASGGHDYGPITELLEEYYGDEETTARICGQFIDPPHCFRI
                     AAVNIQIDSLDPLWQQLFCDLEKQAWEAYPWSSEKTLEQIEDTATGHFDECMMGLPFL
                     AY"
     gene            complement(3349..4377)
                     /locus_tag="P5316_19725"
     CDS             complement(3349..4377)
                     /locus_tag="P5316_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRR87246.1"
                     /translation="MRFLKKLIETPGVPGREERVREVIKAEIKGLFDDVRVDPMGNLI
                     CRKRPGRKSSKPPLKVLIACHIDEIGFYVRHIDDNGFLRVQNVGGFDTRNLFARRVLV
                     QGKKDLVGVLNPAGRPIHIATDEEKKKTYTVGEFFVDLFMSKTQVEKLVRIGDPVTLI
                     QNFEEIGDVVTGKCLDNRAATWVAINAIRKVGKKSPYEIYYAATVQEEVGCRGAGPTT
                     FGIEPDVGIALDTTLCCDTPGIEKNESITAFGKGVALKIMDGRSISHRGLLDEFAAVA
                     EKKKIPYQLEVLPLGGTDAATIQQAGIGRKTVTISIPTRYIHTITEAEHKKDLQAAID
                     LLAAWLGG"
     gene            complement(4541..5440)
                     /gene="ilvE"
                     /locus_tag="P5316_19730"
     CDS             complement(4541..5440)
                     /gene="ilvE"
                     /locus_tag="P5316_19730"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HRR87247.1"
                     /translation="MNFDKPNPFDPRPDLKIWFNGRIVPVAEAKISVFDHCILYGDGV
                     FEGLRVYDGRVFRLAQHLRRLEHSARAIRLELPMTLDEMTRAVNEAVTANNVRDGYVR
                     LVVTRGVGYLGLSHTRTANPTVFIIADQIELYPKELYEKGMAVISASVVRNHPNAVAP
                     RIKSCNYLNNILAKIEGLDAGVYEAIMYNHLGYVAECTGDNIFLVRDGGIQTPPITAG
                     ILEGVTRDVVIELARELKIPLREMDLTRHDLYVADECFLTGSAAEVIPVTKIDGRPIG
                     DGNPGPVTRRLMSSFRELVRRGG"
     gene            complement(5484..>5681)
                     /locus_tag="P5316_19735"
     CDS             complement(5484..>5681)
                     /locus_tag="P5316_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HRR87248.1"
                     /translation="RPQVLLADEPTGNLDAATGKEILGVLKTLNQEGQTIVMVTHDPV
                     VAAAAHRIVHLADGRVTNRKA"
BASE COUNT         1056 a         1737 c         1742 g         1146 t
ORIGIN      
        1 cagcgaagca tgagccatca tgcttccctg ccagttaaaa tgaggctcca cgttggcgtc
       61 atagccgccg tggcagacct cgcaagccgc cgggtcattc agcgttccgg cctcgaaagg
      121 ctgactgccg ggcatgtcga agtcgcggag cgttgtgggg gcgataccgc cgggcgctcg
      181 ttcaacggtg aacggggcat cactctcatc ttgtccggag ttgaaggcgt tgtcgatcgc
      241 caccactcgc acgaacgcgg cggtgctcgg ccggttcggg acgaagaacg tgtgactgcc
      301 gctgttgctc aggcctttgg cgataggctt gaacgtggca ccgttgtcgt ccgagaggta
      361 gatgtccacg gcggctatgc cgctggcgtc cgtggccgtc catagaacga tagcggccgc
      421 gttgccggtg agcgtgtcgc cgccgttggg agacagcacc tgcacagtcg gtggcgtggt
      481 gtcattaccc gcggtgggta ccaggtgggt ctggatctcc gaaacagtga cgttcgagac
      541 attcgagacg tttgccggcg tcaggcccgg aaaggtcggc gtgttggaga attgattgac
      601 agcggtgatc tcgaccacgc ttgtgaagcc gtcgccgtcc gggtcttcgt tgcctatgga
      661 ttggaccgcc tgtctgcgcc cgttgggatt gttggcgaag ttgggcagga cctcgcccag
      721 ccggacgccg tacggattgc gcgtgccgcc gccggtgaaa tcgtagtggc acacgccgca
      781 atgacccgga cgactgggca ccgtgtcgag ggttgatccc acggcgacgg ggtatgcgct
      841 gaaaaacgcg tctcgaatgt tggcccgccc ataagcttcg ccggcggtcg ccgccgccat
      901 cacggcaacg ccgcaatagg caagacgccg actccaaggg cagttgaaaa cgcgggacct
      961 cattgtcctc ctcctctttc cgattgggct gctcgatgcg tcgttgcgat cggccctttg
     1021 tccggtccgc cacgaatgtg ccaggtctct aatcgcccgc acaaaacgga tatgcattcg
     1081 catgcattgt aagaaaaaga ctgcactcga gtaaacgcca aaacaagatt ggggtaattg
     1141 ccgcaagtgg gtcgagatga caaccgtcag catcggattg aagcagacca ttccagtact
     1201 attcactccg cagcacaagt ggctgcttcg cgtcgtgtcg ccggcctcct atcccgaagc
     1261 tcctgcgcgt cccgtttgct tcatgctgtc gtcgcagcct ctctgtcttc agatggtcct
     1321 gcccatcaac ctctcatacc ggcagcggcc agcggagccc cgaccgccgg ccccgaactt
     1381 ccgagacgct tcggtcggga caagctgccc gtgtgatttg tggctgacgc aacctagccg
     1441 ttatcatgtt cccgataaga gaggtgaccc atgagcaacc tgtttgacgt caagggcaaa
     1501 gtcgtggcga ttaccggcgg aggcggagtg ttgtgcggcg gcattgccga atacctggcg
     1561 gcgtgcggga cgaaagtggc ggtgatggat ttgaagctgg acgccgctga agaggtcgtt
     1621 cgcagaatcg tcgccgccgg gggcattgcc acggccatca atgccaacgt tctgcaacgg
     1681 gacagcctcg aagccgctca caagcaatgc gtcgaaacac tcggctcggt cgatgtcctg
     1741 atcaacggcg ccggcggcaa tcacccagcg gccaccaccg ggccggacaa gaagttcttc
     1801 gacctgccca tcgacgcgtt tcagaaggtc atggacctga acatcatcgg cacaatcctg
     1861 ccgtcgatgg ttttcggcag ggcgatggcc gagcgcggcg agggcgtaat cgtgaacatc
     1921 gcgagcatga acgctttcaa accgctgact cgcatcccgg cttactcggc cgccaagagc
     1981 ggggtgaaga acttcaccga gtggctggcc gtgcatatgg cacaggagta ctcaccaaag
     2041 atccgggtca acggggttgc gccagggttt ttcctgacgc accagaaccg attcttgctg
     2101 atggaccagg cgaccggcaa cctgacccag cgcggccaac agatcatcgc tcacacaccg
     2161 atgaaccgct tcggcgaccc gcaggatctt tacggcacga tcatctggct gatttccccc
     2221 gcctcgcaat tcgtcaccgg gatcaccgtg ccggttgacg gtggttacag cgcgttcggg
     2281 ggagtgtgaa gaagggttcg gtggtcagag ttcagggttc agattcccga gggctggcct
     2341 tcgacgcgcg tccgaggttc ggagggcaag aagagggata tcagtacgcc aggaatggca
     2401 ggcccatcat gcattcatcg aagtgcccgg tggcggtgtc ttcgatctgt tccagggtct
     2461 tctcgctact ccacggatat gcttcccacg cctgtttctc aaggtcgcag aagagctgct
     2521 gccatagggg atcgaggctg tcgatctgga tgttcacggc cgcgatccga aaacagtgtg
     2581 gcgggtcaat gaactggcca cagatgcggg ctgtcgtctc ctcgtctcca taatattcct
     2641 caagcaactc ggtgattggg ccgtagtcat gccccccgga ggcggtcaag gcgtcggagg
     2701 cggcgtcgat tcgcgatgag agctggtcgt cgaccaatgg cctgtcgctg ccgccgtcgc
     2761 cctggcacat caagcccata cagaccgccg ccagcaggcc ggcagaggcc aagcgactgc
     2821 ccgcgagacg ccggcgattc gcacggcttg ggtattgtga actcggcatg gcccctcgct
     2881 cccgcggcag caacacatcc tcgcaagtgc ccgccgccct ctcttattcc ataccccccc
     2941 gatggccgag tcaagcacgt tctcgaacag agatgcagaa tgaagctctt ggatgacata
     3001 tcggacgctg atctcgaggt tgcggctgcc cgctgagttc ggttggcatc aatttcaaat
     3061 ctgagattgc agatttgaga tacggctggt cccggattcc catccgggac cactctgctg
     3121 acgattccca tcgtcattcg aggagatggc gtaccgcatg ccgaatggat tcggcgagga
     3181 acgcggcccg gaagggattc cgggccgagc gattggcatc gatcataaat ctgagattgc
     3241 agattcgaga ttcgcacaac atgccagcgt ggaaacggcg gtgcgtcgtg gtgaatggta
     3301 ttcgccccac ccctgtcggg gccaacagcc aagagccaat agcttccttc acccccccaa
     3361 ccacgccgcc agcaggtcga tcgccgcctg gaggtccttt ttgtgctcgg cttcggtgat
     3421 ggtgtggata taccgcgtcg gaatggagat ggtgaccgtc ttgcggccga tgcccgcctg
     3481 ctgaatggtg gccgcgtcgg tgccgcccag cggcagaact tcgagctgat aagggatctt
     3541 ctttttctcg gccacggcgg cgaactcgtc gagcagtccg cggtggctga tggaacggcc
     3601 gtccatgatc ttgagggcca cccccttgcc gaacgcggtg atactctcat tcttctcgat
     3661 cccgggcgtg tcgcagcaca gtgtcgtgtc gagggcgata ccgacgtcgg gttcgatccc
     3721 aaaggtggtc ggcccggccc cccggcagcc gacttcctcc tggaccgtgg cggcgtagta
     3781 gatctcgtaa gggctcttct tgccgacctt gcggatggcg ttgatcgcca cccaggtggc
     3841 cgcccggttg tccaggcact tgccggtgac cacgtcgccg atttcctcga agttctgaat
     3901 caacgtaacc gggtcgccga tgcgaacaag cttttcgacc tgggtcttgg acatgaacag
     3961 atccacaaag aactcgccga cggtgtaggt tttcttcttt tcctcgtcgg tggcgatatg
     4021 gatcgggcgg ccggcggggt tgagcacccc gaccaggtcc tttttgccct gcaccaggac
     4081 gcgccgggcg aagagattgc gcgtatcgaa accaccgacg ttctgcaccc gcagaaagcc
     4141 gttgtcgtcg atatgccgga cgtaaaaccc aatctcatcg atgtggcacg cgatgagcac
     4201 cttaagaggc ggtttggagc tcttacgccc ggggcgcttt cggcagatca agttgcccat
     4261 gggatcgacg cggacgtcgt cgaagagacc tttgatctcg gccttgatca cctcgcgaac
     4321 ccgttcttca cggcccggca cgccgggcgt ctcgatcagt ttcttgagaa acctcatggt
     4381 tcgtccccat agcgaacggc ggccggcgtt cgcgccagcc ggtgaggcag tgtcgagtcc
     4441 gccgcagcgg acgagacctg ccgtctcatc aagcgggggc cggcaggtct gcgccgccct
     4501 tggcggctcc gaccatgccc tacgtgctca acacccgatc tcacccgccg cgacggacca
     4561 attcacggaa gctgctcatc agcctgcggg ttaccgggcc ggggtttccg tcgccgatcg
     4621 gtcggccgtc gatcttggtc acggggatga cctcggcggc cgaaccggtc aggaagcact
     4681 catccgccac gtagagatcg tggcgggtca ggtccatctc ccgcaacggg atcttcaact
     4741 cgcgggccaa ctcgatcacc acgtctcggg tgacaccttc aagaatgccg gcggtgatcg
     4801 gcggtgtctg gatgcctccg tcgcgcacca ggaagatgtt gtcgccggtg cactcggcca
     4861 cgtatcccag atggttgtac atgatggcct cgtaaacgcc ggcgtcgagg ccttcgatct
     4921 tggccaggat gttgttcaga taattgcagc tcttgatccg cggggccacc gcgttgggat
     4981 ggttccgcac gaccgacgca ctgatcaccg ccataccctt ctcgtagagt tccttcgggt
     5041 acagctcgat ctggtctgcg atgatgaaca ccgtcgggtt ggcggttctc gtgtggctga
     5101 ggcccagata gcccacgcca cgggtgacca ccagacggac atatccgtcc cggacgttgt
     5161 tggcagtgac agcctcgttg accgcccggg tcatttcgtc aagggtcatc ggcagttcaa
     5221 gccggatcgc gcgagccgag tgctcgagcc ggcgcaggtg ttgcgcaaga cggaacaccc
     5281 tcccatcgta gaccctcagc ccttcgaaaa cgccgtcgcc gtaaaggata caatgatcga
     5341 agacgctgat cttcgcctcg gctaccggca cgatcctgcc attgaaccag atcttcagat
     5401 cgggtcttgg gtcgaacggg ttgggcttgt cgaaattcat attctgctgt ccgtagaacc
     5461 acaatcgccc cgtcgggggc tcttcacgct tttcgattcg ttaccctgcc atccgccagg
     5521 tgaacgatgc gatgggccgc cgcggccacc accggatcgt gagtgaccat gacaatggtc
     5581 tgaccttcct ggttgagcgt cttgagaaca cccagtatct cctttccggt tgccgcgtcc
     5641 agattacccg tcggctcgtc ggcaagcaag acttgcggac g
//
LOCUS       DAOTPW010000310         5674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12028_length_5674_cov_4.411817, whole genome shotgun sequence.
ACCESSION   DAOTPW010000310 DAOTPW010000000
VERSION     DAOTPW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12028_length_5674_cov_4.411817"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1584..3569
                     /gene="acs"
                     /locus_tag="P5316_19740"
     CDS             1584..3569
                     /gene="acs"
                     /locus_tag="P5316_19740"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRR87249.1"
                     /translation="MSGSAPSTTQERVFMPPPEFTAKAYVKSMEQYQQMYDRSIKDPE
                     GFWGEMASEFYWKEKWTKVREFDFNGDISIKYFIGGKTNITYNCLDRHLDTRGDQVAI
                     IWEGNTPGEDARLTYRQLHTEVCKFANVLKSRGVKKGDRVSIYMPMVKELAIAMMACA
                     RIGAVHSIVFGGFSPESLSDRIVDSGCQTLITTDGVYRGSKAVTLKANADEAMEMSEK
                     QGAPIKTCIVYERVGQDQLPVYMKSGRDFWWHDLMKDASPVCEPEWMDAEDPLFILYT
                     SGSTGKPKGVLHTIGGYMVFVAISHKYIFDYHDGDIYWCTADIGWVTGHSYIVYGPLC
                     NGATTLMFEGIPTYPDAGRFWDVVDKYKVNIFYTAPTAIRALMREGEGPVLKRDLSSL
                     RLLGTVGEPINPEAWMWYYKYVGKERCPIVDTWWQTETGGILITPLPGCTPLKPGSAT
                     FPFFGVKPVLLTPEGKRIEGPGSGVLCIEEPWPGMMRTVYGQHARFKETYFSTYPGLY
                     FTGDGCRRDEDGYYWITGRVDDVINVSGHRIGTAEVESALVSHPAVAEAAVVGFPHQI
                     KGQGIYAYVTLVVGQEYTDALKKELVAHVRKHIGPIATPDVIHWAPGLPKTRSGKIMR
                     RILRKIAEGEPDKIGDTTTLADPTVVKNLVETYKPLS"
     gene            3703..5043
                     /locus_tag="P5316_19745"
     CDS             3703..5043
                     /locus_tag="P5316_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HRR87250.1"
                     /translation="MTNDSISAPAPLMNRWLVVIGAVLIQLCLGAIYAWSVFTPPLTA
                     EPFNFSKVQTQVIFSVGLAAFALVMIKAGMWQAKLGPTRVARLGAVVLGLGYILGGLA
                     SMSLTGPSAFCAILVTVGLIGGAGIGLAYVVPIAVGVKWFPDKKGMITGLAVAGFGFG
                     ALIWIYLGQGLPDWTHIEWDGLIKPLGVGKVFLIFGIAFLVLILVGSIWMKNPPENYR
                     PAGWEPASTPGAAGSSSAQFDWDQMLRTPQFYMLWAMFIFGALAGLMVIGIIKLFGID
                     ALQAGGMDPRQASLTAGTAMALFYALANGFGRIIWGTVSDKLGRKASLVLMMGAQGIV
                     MLLFFHMGGTPALLYLGAALVGFNFGGNFALFPAATADFFGNKNVGKNYPWVFSAYGI
                     GGIVGPIMAGRFGDAVGSRGVEAWKPAFIIAGVACLIAAVIGLMLKPPRPKAIA"
     gene            5159..5488
                     /locus_tag="P5316_19750"
     CDS             5159..5488
                     /locus_tag="P5316_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87251.1"
                     /translation="MSAWLNQRFHADCVDAVTEPGPDGLFSRSQYSLIESIRRRMDIS
                     VNAHGSRVVAHHDCAGNPVERDQHLRDLACALRCMGSWHLPVRLLGLFVNERWQVELI
                     WDTGGSA"
BASE COUNT         1062 a         1721 c         1716 g         1175 t
ORIGIN      
        1 ctgtacccca gatgttcttc tgccccttgg ccgaccattc atctgcgaac tctcccatgg
       61 gcgaagaggg ggtgatcgga taaatcgcga taacctcact gagccggtgc gccaccgagg
      121 ccgccgcttc attgccatct atcgtggtga tgtttctttg agccattcga gatcactcct
      181 ttggaaaccg attcccgctt cggctgaccg aggccggccg atcgtcgttc tcggttgaac
      241 gtagctggat tcattttcgg gggccagcgg ttacccccgt cttgcggatg cacgatcttg
      301 ccggcttcga gccggttcca tcgtagccgc gtactgtaac aggaatagtg atggtcttcc
      361 agcagtcgcg agccggtttg cttttcggcc tctgcgacag tccgggcggg ggtaccactg
      421 ggtgcttttc ccccagcgtc ttctctcgcc gcgccaacac acgcggttgg tccgtcggcg
      481 tagaccggga agccatcagt ccagaagacc caagccgccc aacaatacct gcctttggtc
      541 tggcgggcct cattagaacc ttacggtcag gcatctgtgc ggcttgcgcg catgtttctg
      601 gatcgcagaa cgcttggttg cggcccgcaa aaaggcggtc cgcctcagaa ccttacggcc
      661 ggatatctgt gcggcttgcg cgcatgtttc tggatcgcag aacacttggg ttgctgccca
      721 caaaaaggcg gttcgcctta gcttctctga ccgggtccga aacacccaca gacgcgatcg
      781 cggcctcggt gcgctcgtgt cctggcgaaa aatggcggaa aagcgacgat cagcatcgcc
      841 ggggtagaga cacggcgcct tatgcagcag cgcctatcta cccaacctca acacaccggt
      901 gtcggcccac cccctgcatc gatgagattc ggggcccttg tcgttcacaa attcctacca
      961 taaaacaagt tgtgttaacg agggcaagaa gaaaccgccg ggctgggcgt tgaaatccct
     1021 gtggacacac cgcaaggcac atctcttggc ggttgcctga caatcccaat ccccaacctt
     1081 gaatcttggc aggatccccg gtggcgggcc ttacggttgt gcaaccgctt tggcgttctg
     1141 acctgccttc atcccgctcg cggcggtggt gcgaggttgc ggggtcgggc tctccttcga
     1201 gggtaatcac cagacctcgc gctcgcacga caaggctgcg tctgctgttg ccggcgacgc
     1261 cgttgcacgg taactcgaca gcgtctaaaa tgcggcccct cccggattcg actccgggat
     1321 gcaccgcatg ggcgaaccgg ctttgctccg cccggacaca agcggagctt ccgggatcgc
     1381 gacagagaaa cccattgtgg gcgcctcccc ggccgtggta ttcgttaacg ataccttggc
     1441 tggacctctg tcggcggatg acggtcttga ggaaaacacc ggcctgatcg ggcacgaccg
     1501 cgaaccggcg tgctcgcgag agacgactaa cgtacggttg tctccgacag gcgttcattt
     1561 tctgactgaa ggagcttgtc tgaatgagtg gcagtgcacc atccacgact caagaacgcg
     1621 tctttatgcc gccgcccgaa tttacggcca aggcgtatgt caagtccatg gagcagtatc
     1681 agcagatgta cgatcgctcc atcaaagatc ccgagggttt ctggggagag atggcctcgg
     1741 agttctactg gaaagaaaag tggacaaagg tccgagagtt cgatttcaac ggcgacatca
     1801 gcatcaagta tttcatcggc ggcaagacga acatcactta caactgcctg gacaggcatc
     1861 tcgacacgcg cggcgaccag gtcgccatca tctgggaagg taacaccccc ggcgaagacg
     1921 ccagattgac ctatcgccag ctccacaccg aggtctgcaa gtttgccaac gtgctcaaga
     1981 gccgcggtgt caagaaaggc gaccgcgtct cgatctacat gccgatggtc aaggaactgg
     2041 cgatcgccat gatggcctgc gcacgtatcg gcgcggttca ctcgatcgtg ttcggcggat
     2101 tcagccccga gtcgttgtcc gatcgcatcg tcgattccgg ttgccagacg ctgatcacca
     2161 ctgacggcgt ctaccgaggc tcaaaagccg tcacccttaa ggccaacgcc gacgaagcga
     2221 tggaaatgag cgagaaacag ggcgccccta tcaagacctg tattgtctat gaacgcgtcg
     2281 gccaggacca gttgcccgtc tacatgaagt ccggtcgcga tttctggtgg cacgacctca
     2341 tgaaggacgc ttcacccgtc tgtgagccgg agtggatgga cgccgaggac cccctgttca
     2401 ttctctatac ctcgggttcc accggtaagc ccaagggcgt tctgcacacc atcggcggtt
     2461 acatggtgtt cgtcgcgata tcgcacaagt acatcttcga ctaccacgac ggcgacatct
     2521 attggtgcac ggcggacatc ggttgggtta ccgggcattc ttacattgtc tatgggccgc
     2581 tttgcaacgg cgctaccacc ctgatgttcg aaggcatccc cacgtatccc gatgccggac
     2641 gcttctggga tgtggtggac aagtacaaag tgaacatatt ctacacggcc ccaaccgcca
     2701 tccgcgcgct gatgcgagag ggcgaaggcc cggtcctgaa gcgagatctg tcgagcctcc
     2761 ggctgctcgg tacggtcgga gagccgatca accccgaagc ctggatgtgg tattacaagt
     2821 acgtgggcaa ggaacgctgc ccgatcgtcg acacctggtg gcagaccgag acgggcggta
     2881 tcctcatcac gccgctgccc ggctgcaccc cgctgaaacc cggttccgcc acgttcccct
     2941 tcttcggcgt caaaccggtc ctgctcaccc cggagggcaa gcgcatcgaa gggccgggat
     3001 ccggcgtgct gtgcatcgag gagccgtggc cgggcatgat gcggacggtt tacggccagc
     3061 atgcccgctt caaggaaacg tacttctcga cgtatcccgg cctgtatttc accggtgacg
     3121 gctgccgccg tgatgaagac ggctactact ggattaccgg tcgcgtcgat gacgtcatca
     3181 acgtttccgg ccaccgcatt ggtacggccg aggtcgaaag cgccctcgtg tcacaccccg
     3241 ccgtggccga ggccgccgtg gtcggattcc cccatcagat caaggggcag ggcatttatg
     3301 cctatgtgac cctcgtcgtc ggccaggagt acaccgacgc gctcaagaaa gaactggtcg
     3361 cccacgttcg aaagcacatc gggccgattg ccacgcccga cgtgattcac tgggcaccgg
     3421 gccttcccaa gacgcgttcc ggcaagatca tgcgacgcat tctccgcaag attgccgagg
     3481 gcgagccgga caagatcggc gacaccacca cgctcgccga tccgacggtc gtcaagaacc
     3541 tcgttgagac gtataagccg ctctcgtgac ctgacagtca ccggccgtgg ggatggtcga
     3601 cgaccgtccc cacggccttt tgtctcattg acgagaaaaa accagatctg gtacacagcc
     3661 aacgcatttc ccccgaggag ccgttggctt ggagcatctc acatgacaaa cgactccatc
     3721 tcagctcccg cgccgcttat gaaccgctgg ctggtcgtca tcggcgccgt cctcatccaa
     3781 ttgtgtcttg gggcgatcta tgcctggtcc gtctttaccc cgccgctgac cgctgagccg
     3841 ttcaacttca gcaaagtcca gacccaggtc atcttctcag tggggcttgc ggctttcgcg
     3901 ctggtcatga tcaaggccgg tatgtggcag gcgaagttgg gcccgacgcg agtggccagg
     3961 ctcggtgccg ttgtccttgg cctcggatac attctcggcg gtcttgcgag catgtcactg
     4021 accggaccaa gtgcattctg tgccatcctg gtgaccgtcg gattgatcgg cggcgcgggc
     4081 atcggcttgg catacgtcgt cccgatcgcc gtaggcgtaa agtggttccc ggataagaag
     4141 ggcatgatca ccggtctcgc ggtggccggt ttcggtttcg gagccctgat ctggatctac
     4201 ctcgggcagg gattgcccga ctggacgcac atcgagtggg acggactcat caagcctttg
     4261 ggtgtcggga aggtgttcct gatctttggc atcgccttcc tcgtgctcat tctcgtcggc
     4321 tcgatctgga tgaagaaccc gcccgagaac tacaggcccg ccggttggga accggcttca
     4381 acgcccggcg cggccggctc ttcatccgcc caattcgatt gggaccagat gctcagaaca
     4441 ccccagtttt acatgctgtg ggctatgttc atcttcggcg cgctggccgg cctgatggtg
     4501 atcggcatca tcaaactctt cgggatcgac gcattacagg ccggtggaat ggatccccgc
     4561 caggcatccc ttaccgcagg cacggccatg gctctctttt acgccttggc caacggtttc
     4621 ggccggatta tctggggtac cgtctcggac aagcttggtc gaaaggcctc gctggtgctc
     4681 atgatggggg cccagggcat cgtcatgctt cttttcttcc atatgggagg cacgcccgcc
     4741 ctcctctatc tcggagcggc cttggtgggc ttcaacttcg gcggcaattt cgctctcttt
     4801 ccggccgcga cggctgactt cttcggcaac aagaatgtgg gcaagaacta cccttgggtc
     4861 ttcagtgcat acggcatcgg cggaatcgtc ggccccatca tggccggccg gtttggcgat
     4921 gccgtcggct ccaggggcgt cgaggcgtgg aagccggcct ttatcattgc cggtgttgcc
     4981 tgtctcattg cggcggtaat cgggctgatg ttgaagccgc caagaccgaa ggccatcgcc
     5041 tgaccgtggt ggacatcgtg gacggtcgcc gtcgtcgggg aggtgtcaat gccacacggg
     5101 gccttcgtga ccgtagtcaa ctgcatggat ggacggcgat ggacgggttc aggagccggt
     5161 gtccgcttgg ctgaaccagc ggttccacgc ggactgcgtg gacgccgtca cagagcccgg
     5221 ccctgacggc cttttcagca gaagccagta cagccttatc gagtcgatcc gccggcggat
     5281 ggacatttct gttaatgccc atggctcgcg cgtggtcgcc catcacgatt gcgccggcaa
     5341 ccccgtggag agggaccagc acctgcggga tctcgcatgc gcattgcgat gcatggggtc
     5401 gtggcatctt cccgttcgcc tcctcggtct tttcgtcaac gaacgttggc aggtcgagct
     5461 gatttgggac accggcggca gcgcctgagc gaggcggttc cggcctggtg acttggtggt
     5521 gaagaggccc tgatagactg cggaatatgc ggagtacacg gaaggcgatg agcggatggc
     5581 cgggaaaagg cggggggcgc ttcgccagtt gtcggtggcg gggagggcga agtcgtcagt
     5641 gatgagtttt cagtggtcag tgggtggggg aagg
//
LOCUS       DAOTPW010000311         5663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12063_length_5663_cov_4.594330, whole genome shotgun sequence.
ACCESSION   DAOTPW010000311 DAOTPW010000000
VERSION     DAOTPW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12063_length_5663_cov_4.594330"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="P5316_19755"
     CDS             complement(<1..1153)
                     /locus_tag="P5316_19755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR87252.1"
                     /translation="MAHETPQLAPEQKPRWYQGLTGYQWWVVCVGSLAWLMDTFSQRL
                     FVLARTPALMDVLGASATADEVKAYANYATAAMMLGWAVGGFYFGIVGDKWGRAKTLS
                     ASVLVYSIFTGLSGFAAGGWDFCVWRFLMGCGIGGAFATGATLIAETVPPHSRAFALG
                     MFQALSAVGNITASAIGRFLILPGTPVLQGILGENGIAGWRMLFFVGALPAILVVFIM
                     TTIREPQSWLEARKVAASDKLHKQMGDIKGMFTDPRWRRSTLVGVALAVAGVVGLWGV
                     GFWSPELIRQALPNATSQQIDRVIGLGTLLQDVGAFFGMFVFTLIATLLGRRIGFGVS
                     FLAAFIVVSGVFLTLREAWHAYLMLPMVGFVTLSVFGGYSIYFPEIYPTR"
     gene            complement(1590..2735)
                     /locus_tag="P5316_19760"
     CDS             complement(1590..2735)
                     /locus_tag="P5316_19760"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR87253.1"
                     /translation="MQHEPVDRVPVMCQLALGHYFLHSGRKPSEIWFDTPTFIETLAD
                     FQRRYGFDGFLINLPGRPANWRDYLQTAEPHDNGEYLVWRFGMETFVPADDNPHTFLV
                     GHRDLPRADYITVDPDDPATFRLPGYVWNTWHAPILWDIPDDADLTRPDVYPDWLTRG
                     LRQARVACPDVSVHIEVFSPFTHLLELFGYQSALIALIEAEEACHRMLDQFARQVSAQ
                     VECFASCLPDAVLISSAFAGGGFISREMYVDFVVPYERRVVEAIHKHALPAYVHTCGK
                     IGDRLDLMAETGVDGIDTLDPPPLGTVDLAKAKAEFGKRFFFKGNLDAVNEMLRADDA
                     TFENAVRQRLAVGSRGSGYILSSACSVAPAVRPERLKRMVELSQECA"
     gene            2928..3329
                     /locus_tag="P5316_19765"
     CDS             2928..3329
                     /locus_tag="P5316_19765"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRR87254.1"
                     /translation="MRNCLVSACVTLGLLRAITAAENPQTYLAKRVIEPPAIDGRLED
                     KACKAAPLITGLVSNKRQPAKFATEFRLVRAAEAIYVSVPACEPDISRLKCRDEADAP
                     HRVWNDDCIEIFLDPQATRQRYYQCIINARV"
     gene            3305..3703
                     /locus_tag="P5316_19770"
     CDS             3305..3703
                     /locus_tag="P5316_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87255.1"
                     /translation="MHYQRQGLIACSYCGDFSADLPAEAAAGRTNDAWTLEVRIPFRT
                     FGAAPAPGERWGMNLTRGREPKAEGEQRENSLWCPTDGNHGNPVRFGYLVFGRLTDEV
                     SPSADPDMEVVNRAFDQIGKEVGGRHPACP"
     gene            3831..4373
                     /locus_tag="P5316_19775"
     CDS             3831..4373
                     /locus_tag="P5316_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87256.1"
                     /translation="MWVACSASYFPDMGDILDYPVVSGTLLPSLARQVHARGYKILSY
                     GNPHAGVERPEVYRRTYGLALRVAGYDGAIDYEYRTMDDKAAWDDFDHPHYRDHNFAY
                     PAVGKPVDTIRFAGWREAVDDLRYAATLEWAIWQYRQARREVPQVEESATWLRRLTGK
                     EEPDGVRAEMIRRIDALMGP"
     gene            4618..>5663
                     /gene="ugpC"
                     /locus_tag="P5316_19780"
     CDS             4618..>5663
                     /gene="ugpC"
                     /locus_tag="P5316_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRR87257.1"
                     /translation="MAEVVLENVTKVYPGPVKAVDNVSLRIHDKEFVVLVGPSGCGKS
                     TTLRMVAGLEEVTSGTIRIGQRVVNDVPPKDRDIAMVFQNYALYPHMTVYKNMAFGLK
                     LRRVPKAEIDQRVRAAARLLGIEDLLDRKPKALSGGQRQRVAVGRAIVRTPKAFLFDE
                     PLSNLDAKLRVEMRAELKKLHRRLSTTTIYVTHDQEEAMTLGDRVVVMRDGVVHQCAA
                     PLEVYDLPVNRFVAGFLGTPPMNFFSGRIVAENGSLWFDEGSSRIRMPERLEKPLSGR
                     VGEAMVLGVRPESLSCSNEGRFAGQGNRIDVTISVIEPLGDKMDLYVQTGSHAHVVCR
                     IEADRSLKDGMQMP"
BASE COUNT         1135 a         1588 c         1875 g         1065 t
ORIGIN      
        1 gccgcgtggg gtagatctcg gggaagtaaa tcgagtagcc gccaaacacg ctcagcgtca
       61 cgaacccgac catcggcagc atgaggtaag catgccatgc ctcgcggagc gtcaggaaca
      121 cgcctgagac cacgatgaac gcggcgagga aagacacgcc gaatccaatt cgtcgcccca
      181 acagcgtggc gatcagagtg aacacgaaca tgccgaagaa ggcacccaca tcctggagga
      241 gagtgcccaa tccgatgacg cgatcaatct gttgggaagt ggcattgggg agtgcctggc
      301 ggatcagttc gggtgaccag aatccgacgc cccagaggcc gacgacgccg gcgacagcca
      361 aggcgacgcc gaccagcgtg ctgcggcgcc atcgcgggtc ggtaaacatt cctttgatat
      421 cgcccatctg cttgtgaagt ttgtccgaag cagcgacctt tcgagcctcc agccagctct
      481 gcggctccct gatcgtggtc atgatgaaga cgacaaggat ggcgggcagc gccccgacaa
      541 agaacaacat tcgccagccg gcgatgccat tctccccgag gattccctgc agcaccggcg
      601 taccgggcag gatgagaaag cgtccgatcg ccgacgcggt gatgttgccg acggccgaga
      661 gggcctggaa catgccgagc gcaaaagctc gcgaatgcgg cgggacggtc tcggcgatca
      721 gcgtggcacc ggtcgcgaag gccccgccga taccgcagcc catcaggaat cgccacacac
      781 agaaatccca tccgcctgct gcgaagcctg acagaccggt gaagattgaa tagaccagga
      841 ccgatgccga gagggtcttt gcccggcccc acttgtcgcc cacgataccg aagtagaagc
      901 cgccgaccgc ccagccgagc atcatggctg cggtggcgta gttggcgtag gccttgactt
      961 cgtcagccgt cgccgatgct ccgagcacat ccatgagtgc cggcgttcgg gcaagcacga
     1021 agagccgctg ggagaaggta tccatcagcc aagccagcga gccgacgcag acgacccacc
     1081 actggtagcc ggtgagcccc tgataccatc tcggcttttg ttcgggagcc agttgtgggg
     1141 tctcgtgagc catagagaaa cctcgcttgt tgaaccacag cccttgttgg gcgtcgcgac
     1201 gccggccgcc caaggcgcac tttacgttcg ggaatgctaa tggatgagcg tgggggtgtc
     1261 catgttgatg gcttggacct cggcaggtaa cagcatcacc tcgagcgcgc aaaaatgccg
     1321 aagcggcatg cacaaggagg ttaggcgctc cggcgaaccg gattgtggtc gctcttggcg
     1381 aaagccctga cgccccagga caatgacctc gaacctggta cttgggcccg tgctatatcg
     1441 cttcggcaac attcagtatg gcacatgctc cacgggattt caagttgttt ttgcgatttt
     1501 tggacgaatt cgattggtcg ccgtcaaggt cggacggggg agaagggggg ggcacgtatt
     1561 gcgaagcagg ggactttgcc ggcgttgatt catgcgcatt cctgggagag ttcaaccatt
     1621 cgcttgagcc gttctggtcg tactgcgggg gctactgaac acgccgacga gagaatgtag
     1681 cccgagcccc ggctgccgac tgcaagtcgt tgtctgacgg cgttctcgaa cgtcgcatcg
     1741 tcggcccgga gcatctcgtt cacggcgtcg aggttgccct tgaagaagaa tcgcttcccg
     1801 aactccgcct tggctttggc caggtccacg gttcccagcg gcggcgggtc cagcgtgtcg
     1861 atgccatcca cgccggtttc agccatcagg tcgagccgat caccgatctt gccgcaggtg
     1921 tgcacatagg ccggcaaggc gtgcttgtga atagcctcga caacccgacg ctcgtatggc
     1981 acgacgaaat cgacgtacat ctcgcggctg atgaaacctc cgcctgcgaa agccgagctg
     2041 atgagaacgg cgtcgggcaa gcaagaggcg aaacattcga cttgggcact aacctgcctg
     2101 gcaaactggt cgagcatccg gtggcaggct tcttcggcct caatcagtgc gatgagggcc
     2161 gactggtatc cgaacagttc gagcaggtgc gtgaacggtg agaagacctc gatgtggacg
     2221 gagacgtcgg ggcaggctac ccgggcctgc cgcaggccgc gggtcagcca gtcaggatag
     2281 acatccggcc gcgtcagatc agcgtcatcc ggaatgtccc ataatatcgg cgcgtgccac
     2341 gtgttccaga cgtagccagg gaggcggaaa gtggcaggat cgtcgggatc gaccgtgata
     2401 tagtcggcgc gtggcagatc gcggtggccg accaggaagg tgtgcgggtt gtcatcggct
     2461 ggaacgaaag tctccatgcc gaagcgccaa acaagatact cgccgttgtc atgcggttcg
     2521 gcggtctgca ggtagtcccg ccagtttgcc gggcgtcccg ggaggttgat aagaaagccg
     2581 tcaaaaccgt atcgccgctg gaaatccgcc agggtttcga taaacgtcgg tgtgtcgaac
     2641 cagatctccg aaggcttgcg gccgctgtgc aggaaatagt gtcccagtgc cagttggcac
     2701 ataacgggca cccggtccac cggctcgtgt tgcatggctg ctcgaattcg gtcccgtgag
     2761 gtcatggcgc agatgatagc cgcagacgag tggttgtcct acccttcctg atgcgcgaag
     2821 acgcgccgag ggggcgtcgg caccactcgt gaacctgacc cagccgttgt ccggcgtgcg
     2881 gccgaaaggt aggacttcgg ttgcggccga acggggagga tgcttacatg agaaactgcc
     2941 tcgtatccgc ttgtgtgacg ttggggttgc tacgcgcgat cacggcggcg gagaatcccc
     3001 aaacatacct tgccaagcga gtgattgagc cacccgcgat tgacgggcgc ttggaggata
     3061 aagcctgcaa ggccgctcca ctcattaccg gccttgtgtc caacaagcgg cagccggcaa
     3121 agttcgcgac cgagtttcgg ctcgtgcgcg ctgccgaggc gatatacgtt tcggtgccgg
     3181 cgtgcgagcc ggacatatcc cgcctcaagt gccgcgacga ggcggacgca ccgcacagag
     3241 tgtggaatga tgactgtatc gaaatcttcc tcgatcctca ggctactcgg cagcgatact
     3301 accagtgcat tatcaacgcc agggtttgat cgcgtgcagc tattgcggtg acttctcggc
     3361 cgacctgccg gccgaggcgg cagcgggtcg aacgaacgac gcatggacgc tcgaggtgcg
     3421 gattcccttc aggactttcg gagctgcgcc ggctcccggt gagcggtggg gcatgaatct
     3481 gacgcgcggg cgtgagccga aagccgaggg agaacagcgg gagaactcgc tctggtgccc
     3541 gacggatggc aatcacggca atcccgtgcg gttcggatat ctcgtgttcg gccggcttac
     3601 tgatgaagta tcgccgtcgg ccgaccccga catggaggtc gtcaatcgcg ctttcgatca
     3661 gattggcaag gaggttggcg gccgtcaccc ggcatgcccg tgaagccacc gcttgggcgt
     3721 tgaggaacgg tcacccggcc tattgcttcc aggggcgcga cgaggcgacc ggagaggcct
     3781 tgcgctctca gtgtccagcc tgggaggcag cccgtgccgg tggtggcaag atgtgggttg
     3841 cctgttcggc aagctacttt ccggacatgg gtgacatcct cgactatccg gtggtgagcg
     3901 gcacgctctt gccttcgctt gcaaggcagg ttcacgcgag ggggtacaag atcctgagct
     3961 acggcaaccc tcatgccggg gtcgagcggc cggaggttta ccgccgcacc tacggtttag
     4021 ccttgcgagt ggccggctac gatggggcaa ttgactacga gtaccggacc atggacgaca
     4081 aagcggcctg ggatgatttt gaccatcctc attaccggga tcacaacttc gcgtatccgg
     4141 ctgtgggcaa accggttgac accattcggt ttgcgggctg gcgagaggcg gttgatgatc
     4201 ttcgatatgc tgccacgttg gagtgggcca tttggcagta tcgtcaggct cgacgcgagg
     4261 ttccgcaagt cgaggagagt gcgacgtggc tgaggcgcct gaccggcaag gaggaacctg
     4321 acggcgtcag ggctgaaatg atccgccgca tcgacgctct gatgggtccg tgagcggacg
     4381 ccagcctcgt tgcccgccta cctcgcggcg gcgacggcta tcgatcgctc gtaactcatg
     4441 tatggacaga atgctgaggc tgctcgccgc acctgggctt cagaggtcgg gagtccggct
     4501 tgaccgcttg gctgcgatgt gtagcgtcgt tgacagcggc gaggcaccaa ctataatcgg
     4561 aaccatgaca gggaccgagg cggctcatta tgcacgtcgc tttggagtgt agcttttatg
     4621 gcggaagtgg tccttgaaaa cgtgacaaag gtctatcccg gcccggtgaa ggcggtggac
     4681 aatgtgtcac tgaggattca cgacaaggag ttcgtggttc ttgttgggcc ttcggggtgt
     4741 ggcaagagca cgacgttgcg gatggtggcc gggttagagg aagtcacctc cgggaccatt
     4801 cgcatcggcc agcgggtggt gaacgacgtt ccgcccaagg atcgtgacat cgccatggtc
     4861 ttccagaact atgccctgta cccgcacatg acggtgtaca agaacatggc ctttgggctc
     4921 aagctgcggc gggtcccgaa ggcggaaatc gaccagcggg tacgtgctgc cgcgaggcta
     4981 cttggtatag aggatctcct ggaccgcaag ccgaaagcgc tgtcaggcgg ccaaaggcag
     5041 cgggtcgctg tcggacgggc gatcgtcaga acccccaaag cgttcttgtt tgacgagccg
     5101 ctgagcaatc tggatgcgaa gcttagggtt gaaatgcggg ctgagctgaa gaaactgcat
     5161 cggcggttgt cgacgacgac gatttacgtc acccatgacc aggaagaggc catgacgttg
     5221 ggtgatcggg tggtggtcat gagggatggc gtggtgcacc agtgtgcagc cccgctggag
     5281 gtttatgatc tgccggtcaa ccggtttgtt gcgggatttc tgggcacgcc gccgatgaat
     5341 ttcttttcgg gacggatcgt ggccgagaac ggatccttat ggtttgacga gggttcgagc
     5401 cggattcgga tgccagaacg tctggagaag ccgctcagcg gacgggttgg tgaagcgatg
     5461 gtcctggggg ttcgcccgga gagtctgtcc tgctcaaatg agggcaggtt cgcaggtcag
     5521 ggcaacagga tcgacgtgac gattagtgtc atcgagccgt tgggcgacaa gatggatttg
     5581 tacgtccaga ccgggtcgca cgctcatgtg gtttgccgga tcgaagcgga ccggtcattg
     5641 aaggacggca tgcagatgcc cat
//
LOCUS       DAOTPW010000312         5592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12241_length_5592_cov_5.578653, whole genome shotgun sequence.
ACCESSION   DAOTPW010000312 DAOTPW010000000
VERSION     DAOTPW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12241_length_5592_cov_5.578653"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1040)
                     /locus_tag="P5316_19785"
     CDS             complement(75..1040)
                     /locus_tag="P5316_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRR87258.1"
                     /translation="MKRKILRADAILVLAALLWGVAFVPQRVAMRHMGPLTFTGVRFA
                     LGAMVLVPVIAVRSRRWTVGEPIAVGPTGDGWGRCLVGGGLAGLVLFVAVNLQQAGLV
                     YTTAGKAAFITGFYVPLVPVLGLLVGQHTSIATWVGAVLSVVGLYFLSVVESLEINRG
                     DLLEMIGAVVWAIHVLLIGRFAPRTDPLRLGLVQFVTVAIVSSIGALPTEGISAVGLR
                     AAIYAILYGGIVSAGIAYTLQLFGQRDAPPGHAALVLSLEAVFAAVAGIMILNERFEP
                     REWFGAAVMLGGMLISQVNRFRSGTGTVCERVGQANPPEGSAVLD"
     gene            complement(1271..2935)
                     /locus_tag="P5316_19790"
     CDS             complement(1271..2935)
                     /locus_tag="P5316_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HRR87259.1"
                     /translation="MAAYKFPRLPKTPKVGKKQVLMVASGDLRLSANQVCWPAQHEME
                     QALSKAVADFGYELVRAHPYKRNEKHGFISSQVEGMKVFAGIDSTAPLIVAEAVWQYS
                     HHVLAGLLAHQGPILTCANWSGQWPGLVGMLNLNGSLTKAGKKYSTLWSEDFSSKEFR
                     AGLQRWLKTGSVTHPMKHVTPFSKVRIPAKERALGEALADQLRREKAIMGVFDEGCMG
                     MYNAIIPDELLNPTGVYKERLSQSALYYETTQVSDREAREIRKWYDRKGMTFVTGPDE
                     KKHLTEKQILTQCKMYIAAMRIADDFGCHTIGIQYQQGLKDLLPASDLVEGTLNNVDR
                     PPVYSRDGKRLLYPGEALPHFNEVDECCGLDCLMTYRIHRAMGQPPENTLHDVRWGDW
                     DRSGTVDDYVWVFLISGSVPPAHLIGGWKGASSERQPAMYFRLGGGTIKGVSKPGEIV
                     WSRVFIEDGRLKIDIGRGGVVRLPKEETERRWEATTSQWPIMHAITYGVSRDEFMAKH
                     KANHIQVAYARDAKAADNAVLVKAAMAAAMGMEVNLCGTRKDGKRW"
     gene            complement(3100..4044)
                     /locus_tag="P5316_19795"
     CDS             complement(3100..4044)
                     /locus_tag="P5316_19795"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRR87260.1"
                     /translation="MERFSRFKIPVAVLILGGLPAIALMVISRLEPEHPEPLTSPVPA
                     TGPGEQIGLWQPLFVGVEMCRASTSIPRPMQIRAVRVDCREPTIDFLVTPSNGDEPLD
                     CNARAPSEFLKEFKCQVAINGSVFSPTASRAREPLDVRGLSLSRGNLYSPPNQYDALL
                     IDRDRKAWIGLSPVAVGRAFNGLSGFHAILLDGRNVGTGNDLEPRSAVGISKDQRYLI
                     LMTIDGRQTNYSEGATTTETAEWLRRLGAWNGLNLDGGGSTALVIEGPDGNPLLLSSP
                     SGKYERWVANHLGVFARRLPISSLPATGPTSRGAEPAG"
     gene            4325..5341
                     /locus_tag="P5316_19800"
     CDS             4325..5341
                     /locus_tag="P5316_19800"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRR87261.1"
                     /translation="MSNKKLYGIFTPNMVPLDDRGRINEPELRRIVDWLIEKGISGLY
                     PNGSTGEFTRFSFEERKEIVRIVAEQNAGRRFLLAGAAEANVEKTIEAAAYYHSLGAT
                     AVAIVAPFYYKISQEGVYAYFSEIARNSPIDLTLYNIPQFANDISNDTIKRLAAEHPR
                     IIGIKDSSRDLPKYVNMMHEIRPIRPDFVFLIGCEEILLPSMIMGGDGGTVATSGVVP
                     EVVMKLYNLTMAGRIEEARQVQYALLDLIKMIIFGADFPEAVRQAVGLRGFKMGRGRQ
                     PLSPSQQFSLEDVARTVQCILNQFGLTDAPPGGCDPLPLGLDRQSLDQIVQDVLRGLR
                     TKRN"
BASE COUNT         1069 a         1835 c         1669 g         1019 t
ORIGIN      
        1 tcgccaaggg agttttcgcg cttgacaaga cgggggctaa tgggtgaatg ctgacagctt
       61 gtcatcctcg gttgtcagtc aagcacggcg ctgccctctg gcggattggc ttgcccgaca
      121 cgctcgcaga ccgtgccggt gcccgatcga aaccggttca cttgtgaaat cagcatcccg
      181 ccgagcatga ccgctgcgcc gaaccattcc cgcggctcga atctttcatt gaggatcatg
      241 atgccggcga cggccgcgaa taccgcctcc aggctgagca ccaaggcggc gtgtccgggc
      301 ggcgcgtccc gctgaccgaa cagttgcagc gtatacgcaa ttccggccga gacgattcca
      361 ccgtagagaa tcgcatatat ggccgcccgc aggcccaccg cgctgattcc ctcggtgggc
      421 agcgccccga tactactcac gatcgccacg gtcacgaatt gcaccaaccc gagtctcagc
      481 ggatcggttc gcggggcgaa tcggccgatg agcagcacgt ggatcgccca taccaccgcg
      541 ccgatcattt ccaaaaggtc gccacggttg atctccagcg attcgaccac gctcagaaag
      601 tagagaccta ctacggatag gaccgccccg acccacgtgg cgatgctcgt atgctggccg
      661 accagcaggc ccagcaccgg caccaggggc acgtagaagc cggtgatgaa ggctgctttg
      721 ccggcggtgg tgtagaccaa acccgcctgc tgaaggttga ccgcgacgaa caataccagt
      781 cccgccaatc ccccgccgac aaggcatcgg ccccacccgt caccagtcgg accgaccgcg
      841 atcggttcgc caacagtcca acgccgagac cgcacggcaa taaccggcac aagcaccatc
      901 gcgcccagcg cgaatcgcac cccggtgaag gtcagcggtc ccatatgccg catggcaacc
      961 cgttgaggca caaacgccac gccccacaga agggctgcca acacgaggat cgcgtcagcc
     1021 cgcaagatct tgcgtttcat ccgaaccacc aggccaaccg tcgaccccac gctcgacagc
     1081 cgtcattgcc ggccatgctc gcgtgcaaca gcaggttttc ggccgtacaa gcgaacggct
     1141 ccggagacag cttgccggag ccgttatgag tattcccctg ttccaagagc ctgacggtcc
     1201 ccgacgagcg gcgtccgccg aaactcgggg atgccggccg ggagctgaaa gccgccggcc
     1261 cgtttgcccc ttaccacctc tttccgtcct tgcgggtacc gcagaggttg acttccatgc
     1321 ccatggcggc cgccatcgcc gccttgacca gcaccgcatt gtccgcggcc ttggcgtcgc
     1381 gggcgtaggc cacctggatg tggttggcct tgtgcttggc catgaattcg tcacggctga
     1441 cgccgtaagt gatcgcgtgc atgatcggcc actgcgaggt cgtggcttcc cagcgacgct
     1501 cggtctcttc tttgggcagt ctgaccaccc cgccgcggcc aatatcaatc ttgagtctgc
     1561 cgtcctcgat gaagactcgc gaccacacga tctcgccggg cttggagaca cccttgatcg
     1621 tcccgccgcc cagtcgaaaa tacatcgccg gctgccgctc gctgctggcc cccttccacc
     1681 cgccgatcaa atgggccggc ggcaccgagc cgctgatcag gaacacccac acgtagtcat
     1741 cgaccgtccc tgaacggtcc cagtcgcccc agcgcacgtc gtgcagcgtg ttctccggcg
     1801 gctgccccat cgcccggtga atccggtagg tcatcaggca gtcaagcccg cagcactcgt
     1861 cgacttcgtt gaagtgcggc agagcctcgc ccgggtacag caaccgcttg ccgtcgcggc
     1921 tgtacaccgg cgggcggtcg acgttgttca gcgttccctc gaccagatcg ctggccggca
     1981 gcagatcttt gagaccttgc tggtactgga tgccgattgt gtggcagccg aagtcgtcgg
     2041 cgatgcgcat cgcggcgatg tacatcttgc attgcgtgag gatctgtttc tccgtcaggt
     2101 gcttcttctc gtccggaccg gtgacgaaag tcatgccttt gcggtcatac cacttgcgaa
     2161 tctcgcgagc ctcgcggtcg ctgacttgcg tggtctcgta atagagggcc gactggctca
     2221 ggcgttcctt gtagaccccg gtcgggttca gcagctcgtc ggggatgatg gcgttataca
     2281 tgcccatgca accctcgtcg aaaacgccca tgatcgcttt ctcgcgacgg agctgatcgg
     2341 ccaaagcctc gccgagggcc cgctccttgg caggaattct cactttcgag aacggcgtga
     2401 cgtgtttcat gggatgcgtc acgctgccgg tcttgagcca ccgctgcaga ccggctcgaa
     2461 actccttcga tgagaaatcc tcgctccaca acgtggagta cttcttgccg gccttggtca
     2521 gcgagccgtt gaggttcagc atgccgacca ggcccggcca ctgcccggac cagttggcgc
     2581 aggtcaggat cggcccctgg tgggccagca ggccggccaa cacgtggtgc gagtactgcc
     2641 acacggcctc cgcaacgatc aacggcgccg tggaatcgat gccggcgaac accttcatgc
     2701 cctcaacctg cgaggagatg aagccgtgtt tctcattccg cttgtaagga tgggcacgca
     2761 cgagctcgta gccgaaatcg gcgaccgcct tggacagggc ctgttccatc tcgtgctgcg
     2821 cgggccaaca gacctgattg gccgacaggc gaaggtcgcc gctggcaacc atcagaacct
     2881 gcttcttacc aacttttggc gttttcggga gcctgggaaa cttgtaagct gccataattc
     2941 ggagttcctt gtataaaaac cgcccggcga catgcccggc ctgagacact atctacacgc
     3001 attggccggg gggtgcaagg caacggcact tgaccagccc gggggactgt cccgcaagcc
     3061 gagggtccgc tcacgggtct ccgatgctgg gtttcggggt caaccggccg gctcagcccc
     3121 ccggctcgtc gggccggtgg ccgggaggga ggaaatgggc aaccttcggg cgaaaacgcc
     3181 cagatggttg gccacccatc gctcgtattt gccgctggga ctgctgagca acaggggatt
     3241 gccgtccgga ccctcgatga ccaacgccgt gctgccgccc ccgtcgagat tgaggccgtt
     3301 ccaggcaccc agcctgcgca gccattcggc ggtttcagtg gtggtggccc cttcgctgta
     3361 attggtctgc cggccgtcga tggtcatcag gatcaggtaa cgctggtcct tgctgattcc
     3421 gacggcggat ctcggttcca agtcgttgcc tgtgcccacg ttgcgaccgt ccagcaaaat
     3481 ggcgtggaaa ccgctcagcc cgttgaaggc cctgccgact gctaccggcg ataggccaat
     3541 ccatgccttt cgatctcgat cgatcagcag agcatcatac tggttgggcg gcgagtagag
     3601 attgcctcgc gaaagagaca agccacggac gtccagaggt tcccgagccc ggctggccgt
     3661 cggcgaaaag accgaaccgt tgatagccac ctgacacttg aactccttca gaaactcaga
     3721 gggagcgcgg gcgttgcagt ccaggggctc atcgccattt gagggggtga cgagaaagtc
     3781 aatcgtcggc tcgcggcagt cgacgcggac tgcccggatc tgcatcggcc tgggaatgga
     3841 cgtagatgcc cgacacatct cgacgccgac aaaaagaggt tgccagagac cgatctgttc
     3901 gcccggtccg gtagcaggca ccggtgacgt cagaggctca gggtgttcag gctccagccg
     3961 gctgatgacc atcagcgcga tcgccggcaa cccgccgaga atcaagacgg caacaggtat
     4021 cttgaatcgc gaaaaccgtt ccacacacgt ccctcgctcg cgctgctgac cggtgaatca
     4081 ctacccttct tatcggctgg aaatgagcat cctgttgatg aacgcggcac tggaccagcc
     4141 gagccgacac cggctcggct tgcggttcct cggcaacatc aaccaggccg gcaggacgcc
     4201 cggtgcaggt ttcgacggct ctcggccgcg tgggcatggc tgtcggggtt caggtgacgc
     4261 ggtcgacggg gtttgcggtt tgcgtgtaga atagtcgacc gttctatgcc cctacggaga
     4321 cgagatgagc aataagaaac tctacggcat cttcaccccg aacatggtcc cgttggacga
     4381 tcggggccgg atcaatgagc ctgaactgcg tcgcattgtc gactggctga tcgaaaaagg
     4441 catctccggc ttgtatccca acggaagcac gggcgaattc acccgcttca gtttcgagga
     4501 gcgcaaagaa atcgtccgga tcgtcgccga gcagaacgcg gggcgacggt tcctactggc
     4561 cggcgccgcc gaggccaatg tcgagaagac cattgaggcg gcggcctact accattcctt
     4621 gggcgccacg gccgtggcca tcgtcgcccc cttctactac aagatcagcc aggagggggt
     4681 gtacgcctat ttcagcgaga tcgcccgcaa cagccccatt gatttgacgt tgtacaacat
     4741 cccccagttc gccaacgaca tcagcaacga cacgatcaag cgtctggccg ccgagcatcc
     4801 acggatcatc ggcatcaagg attccagccg cgatttgcca aaatacgtaa acatgatgca
     4861 tgagatcagg ccgatccgcc ctgatttcgt ctttctgatc ggctgcgagg agatcctgtt
     4921 gccctcgatg atcatgggag gcgacggtgg aaccgtggcc accagcggcg tcgtgcccga
     4981 ggtggtcatg aagctttaca acctgacgat ggccggacgc atcgaagaag cacggcaggt
     5041 ccagtacgct ctgctcgacc tgatcaagat gatcatcttc ggtgccgact tccccgaagc
     5101 cgtgcgacaa gcagtggggc tgcgcgggtt caagatgggg cgcggacgcc agccgctgtc
     5161 gccttcgcag cagttcagcc tggaggatgt cgcccgaacc gtccagtgca tcctcaacca
     5221 gttcggcctg accgatgccc cgcccggagg ttgcgatccg ctcccgctgg gcctcgatcg
     5281 acaatcgctc gaccagatcg tgcaggacgt gctccgcggc ctgcggacca agaggaattg
     5341 accgtccatg cggacgcaac gtaccctgga agacgtgcgc acaatgacgg actcgcccgg
     5401 caagacagca tcgaggtcgc cgcagcgacc gagacctgcc gcgcggtcag gacggcggca
     5461 ggtcttcgcc gcctttgacg attccgaccg tgctctgccg gttgcacgcg cgcccttgcc
     5521 gtgtctgccg ctcttcaccg gcccccacgc cccaaccccc ggcccttcac cttggccaca
     5581 tgaacatcga ct
//
LOCUS       DAOTPW010000313         5579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12263_length_5579_cov_5.843411, whole genome shotgun sequence.
ACCESSION   DAOTPW010000313 DAOTPW010000000
VERSION     DAOTPW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12263_length_5579_cov_5.843411"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..3113
                     /gene="leuS"
                     /locus_tag="P5316_19805"
     CDS             228..3113
                     /gene="leuS"
                     /locus_tag="P5316_19805"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRR87262.1"
                     /translation="MSSERGRYDFAAIEAKWQKYWIEQKTFRTPQPGDADFDPAKPKF
                     YVLDMFPYPSGVGLHVGHPLGYIATDIYARFLRMRGYNVLHPMGYDAFGLPAEQFAVE
                     HGVPPRETTQKNIDNMRAQMQRLGLGYDWDRELATTDVGFYKWTQWIFLKLFNSWYDP
                     EADAARPIDELIHKLEQGMLAVTPDGKVATWPTTSAITGRPVGLSKWEELSPDERRSV
                     IDGQRLAYIDEIPVNWCPALGTVLANEEVTNEGRSDRGNHPVYRRPLRQWMLRITRYA
                     DRLERDLDMVDWPEAIKLMQRNWIGRSEGAEVDFAVDGSEEVIRVYTTRPDTLFGATY
                     MVLAPEHELVARITTRQQRAAVQSYVEEARHRSDLDRTADNKEKTGVFTGAYAINPVN
                     NEKIPIWVADYVLMGYGTGAIMAVPAHDTRDFQFALKFNLPIVQVVQPPGGEDWRGFT
                     GDGTAVNSGRFNGLSTPEFKKRITAWLEEKGLGKGAVNYKLRDWLFSRQRYWGEPFPI
                     VHDTKTGEVLALDESELPVELPAIDDYRPQASDDPNAPPAPPLGRAKDWVSVRGYVTD
                     AGTVKLLKPGQSPPSGLDVREFCRELNTMPQWAGSCWYYLRYLDNRNDRAFVDPQIEK
                     YWMAPVNRPSAPPCGGVDLYVGGAEHAVLHLLYSRFWHKVLFDLGYVSSPEPFGRLFN
                     QGYIQAYAYQDARGMYVDVRDVEERSEGKDARFVRRSTGEVLSRSLGKMGKSLKNAVA
                     PDDICHEYGTDTLRIYEMYMGPLEASKPWNARDIVGMSRFLQAVWRRFIAEDGSLKLT
                     NSPAEDSLRRLLHRTIRKVTGDMQGLRFNTAIAALIELNNAMKGDLLPREVAEPFLKL
                     LAPIAPHLCEELWQRMMGSSWKRSIAFEDWPTYDEALCVEAEVEIPVQISGKVRARIK
                     LPADQAQSESAMQAAAMADEQVRKLLAGKTVRKVICIPGRMVNIVAN"
     gene            3441..3905
                     /locus_tag="P5316_19810"
     CDS             3441..3905
                     /locus_tag="P5316_19810"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRR87263.1"
                     /translation="MDDRHDEIFKALADPNRRRIIAALCSEPMVAGDLGRLVSLAPNA
                     VSFHLRLLQEARLVTVQRQGRFLWYRVDRDVLDDWRSYAHALFEVRAGGESIEAHASR
                     PPEYPPPAELRSAARSPSAQPAETQPVSRRDVPVDRENAAADTDEKLPAELL"
     gene            complement(4080..4700)
                     /locus_tag="P5316_19815"
     CDS             complement(4080..4700)
                     /locus_tag="P5316_19815"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRR87264.1"
                     /translation="MATLTRAASGAKYRTDSSAMTTVPARVVFYEGQRIYFSPIEPED
                     EPLVRRFINDPGNWQGLMHRGPVNSCREREWVESLGRDEKDCVFGIVVREGDRLIGVT
                     GLHNIHAVNRSASFGINIGDRTFQNKGYGTEATLLCLRYGFEELNLNRIALSVFSNNP
                     RAIRCYQKAGFVQEGCLRQAVFRNGRYENEYRFAVLREEWELGRQR"
BASE COUNT         1095 a         1767 c         1717 g         1000 t
ORIGIN      
        1 gcggcgatcg gccgccgatc gcgcaaaact gccaccggag ttcgcttgtt ttgggtctag
       61 caggacgcgg tgttccagcc aagtgcgtcg agccgcgact gcgggcgaac cgcgtcgagc
      121 catgtctttt gctcgcacca ggaacggggg gtatcatgtt tgcctccgcc gggagccgac
      181 catggtcgtc gaagccgggg gggtgatcga accgaagaga ggcagtgatg agttcggaac
      241 gcgggcgtta tgatttcgcc gccatcgagg caaaatggca gaagtactgg atcgagcaga
      301 agacctttcg cacacctcag ccgggagacg ccgactttga cccggccaag ccgaagttct
      361 acgtgctgga catgttcccg taccccagcg gcgtgggtct gcacgtcggc catcccctgg
      421 gctacattgc caccgacatc tacgctcgct tcctgcgcat gcgcggttac aacgttctgc
      481 atccgatggg ctacgacgcg ttcggcctgc cggccgagca gttcgccgtc gaacacggcg
      541 tgccgccgcg cgaaaccacc cagaagaata tcgacaacat gcgggcccag atgcagcgcc
      601 tcggcctggg ctacgactgg gaccgcgaac tggccacgac cgacgtcgga ttctacaagt
      661 ggacgcagtg gatcttcctc aagctgttca acagttggta cgaccccgag gcggacgccg
      721 cccggccgat cgacgagttg atccacaagc tggagcaggg gatgctcgcg gtgacgccgg
      781 atggaaaagt ggccacgtgg ccgactacct ctgcgatcac gggtcgcccc gtcgggctga
      841 gcaagtggga ggaactgtcc ccggacgagc gccggtcagt catcgacggg cagcggctgg
      901 cctacatcga cgagatcccc gtcaactggt gcccggccct cggaacggtt ttggccaatg
      961 aagaggtcac caacgagggc cgcagcgatc gaggtaatca cccggtttac cggcggccat
     1021 tgcggcagtg gatgctgcgc atcaccaggt acgccgaccg gctggaacgc gatctggata
     1081 tggtcgactg gcccgaggcc atcaagctca tgcagcgcaa ctggatcggc cgcagcgaag
     1141 gcgccgaggt cgacttcgcc gtcgatggct ccgaggaggt catccgcgtt tacaccacgc
     1201 gaccggacac gctgttcggg gcaacctaca tggtcctggc gccggaacac gagcttgtcg
     1261 cccggatcac gacgcggcaa cagcgggcag ccgtgcagtc atacgtcgag gaggcaagac
     1321 acagatccga tctggatcgc accgcggata ataaggagaa gaccggcgtt ttcacgggtg
     1381 cctatgcgat caatcccgtc aacaacgaga agattccaat ctgggtggcc gattacgtgc
     1441 tgatgggtta cggcaccggg gcgatcatgg ccgttcccgc ccacgacacc cgcgacttcc
     1501 agttcgccct gaagttcaac ctgccgatcg tgcaggtcgt gcagccgccc ggcggggagg
     1561 attggcgagg gttcaccgga gacggcacgg cggtcaattc aggccggttc aacgggttga
     1621 gcacacccga attcaagaag cggatcaccg catggcttga agagaagggg ctcggcaagg
     1681 gagcggtcaa ctacaagctt cgagactggc tgttcagccg tcagcgctac tggggcgaac
     1741 cgttccccat cgtacacgac accaagaccg gtgaggtgtt ggcactggac gagagcgagc
     1801 tgccggtgga actgccggcg atcgacgact acaggccaca agcctcggac gatcccaacg
     1861 ccccccccgc gcctccgctc gggcgcgcga aagactgggt cagcgttcgc ggctacgtga
     1921 ccgatgccgg cacggtcaag ctgctcaagc ccggtcagtc tccgccgtcc ggcctcgacg
     1981 tgcgggaatt ctgccgcgag ctgaacacca tgccgcaatg ggcgggttcg tgctggtact
     2041 atctgcggta tctcgacaac cgcaacgacc gggcgttcgt cgatccccaa atcgagaagt
     2101 actggatggc gcctgtgaac cgtccaagcg caccgccgtg cggcggcgtc gatttgtacg
     2161 tcggcggcgc ggagcacgcc gtgctgcacc tgctctactc gcggttctgg cacaaggtgc
     2221 ttttcgacct tggctatgtc agttcgcccg agccgttcgg gcggctgttc aaccagggat
     2281 acatccaagc ctatgcgtac caggatgccc gtggcatgta cgtcgatgtt cgcgacgtgg
     2341 aggaacggag cgagggaaag gacgcccggt tcgtgcgacg gtccaccggc gaggttcttt
     2401 ctcgaagcct gggaaagatg ggaaagagcc tcaagaacgc cgtggctccc gacgacatct
     2461 gccacgaata cggcaccgac accctgcgga tctatgagat gtacatgggc ccgctggaag
     2521 caagcaagcc gtggaatgcc cgtgacatcg tcggcatgag ccggttcctg caggcagtct
     2581 ggcggagatt cattgccgag gacggatcgc tgaagctgac gaattcgccg gccgaggatt
     2641 cgttgcgacg cctgttgcac cgcacgatcc gcaaggtgac cggcgacatg caaggcctgc
     2701 gtttcaacac cgcgatcgcc gctctgatcg agctgaacaa cgcgatgaag ggcgacttgt
     2761 tgcctcgtga agtggccgaa ccgttcctca agctgctggc gcccatcgcc ccgcatctgt
     2821 gcgaagagct gtggcaacgg atgatgggct caagctggaa gcgatcgatt gcattcgagg
     2881 attggcctac ctacgacgag gccctgtgcg tcgaggccga ggttgagatc ccggtgcaga
     2941 tcagcggcaa ggtccgcgcg cggatcaagt tgccggctga ccaggcccag tctgaatcgg
     3001 ccatgcaagc cgcggccatg gccgacgaac aggtccgcaa gctactggcc ggaaagaccg
     3061 tccgcaaggt catctgcatc cccggccgga tggtcaatat cgtggcaaat tgagctgcaa
     3121 ccatcggctt gggtgcgcga cagtctgggc gggcatgccg acccgtcccg gttgggcgat
     3181 caacccttct tctcgggcct tccgcttccg ccaagactcc gctggcccca aaatcttctg
     3241 gggttctccg tgttgaggat tcccatcctc attttcagcg aatgcggaaa ggattccgcc
     3301 aagactcgca gcccgaaacg gatttcgggc cgagcggagg cttcccatgg tcccacttgt
     3361 gaagggggaa gcgtcacccg tcttgttact tgacatggag cttccgcctc gtagactctt
     3421 gtttcaagga agcctgaagc atggacgatc ggcacgacga gatcttcaag gccttggccg
     3481 acccgaaccg ccgccggatt attgcagcct tgtgcagcga gccaatggtg gccggtgatc
     3541 tgggccgtct tgtgtcgttg gcccccaacg ccgtatcgtt ccacctcagg ctgcttcagg
     3601 aggcccgact ggtcacggtt cagcgacagg gacggttcct gtggtaccgg gtggaccggg
     3661 atgtgctcga tgactggcga tcttatgccc atgcactgtt tgaggtccgg gccggtggtg
     3721 agtccatcga agcgcacgcg tcacgcccgc cggagtatcc gccgcctgcc gaactgagat
     3781 cagcggcccg cagcccgtcc gctcaacctg ccgaaaccca gcctgtcagt cgtcgcgacg
     3841 tgccggtcga tcgcgaaaac gccgccgcgg acaccgacga aaagctccct gccgagctgc
     3901 tttgagcaac cggagaaatc gcttcctgtc tctgcaacgc ccgggccaca acactgttcc
     3961 aggcgagaag gccctcatct cgcgttcacc ccgcctggcg gggccggacc tgcaggactg
     4021 ggcgattgcc caatgactgc cggcccggcc cgccatccaa cgagtcaccc cgaccgagat
     4081 catctttgcc gccccagttc ccattcttct ctgagtaccg cgaaccggta ttcattctca
     4141 taccgcccgt tccgaaagac ggcctgacgc aggcagcctt cctggacgaa gcctgccttc
     4201 tggtagcacc ggatcgcccg cgggttgttc gagaacaccg acaaggcgat gcggttgagg
     4261 ttcagttcct caaacccgta tctcaggcac agcaatgtgg cctccgtgcc gtagcccttg
     4321 ttctgaaagg tccgatcgcc gatgttgatc ccgaaactcg cgctgcgatt gacggcgtgt
     4381 atgttgtgca ggccggtcac gccgatcagg cggtcgcctt cgcggacgac gatgccgaac
     4441 acacagtcct tctcgtcgcg tccgagcgac tcgacccact cccgctcgcg acaggagttg
     4501 accggaccgc gatgcatcag gccttgccag ttgcccggat cgttgatgaa ccggcgcacc
     4561 agcggctcat cttcaggctc gatcgggctg aagtagattc gctgcccttc atagaagacg
     4621 actcgcgcgg gcacggtggt catggccgaa gagtcggttc ggtacttcgc gcccgacgca
     4681 gcacgggtca gagtcgccat ggcatacact ccgcgggcag cccgcttccc tgcgggctgc
     4741 cctggtaaca gaaccttgca gaagccggcg ctcgcacgcg cgagcgagcc tccgcccttc
     4801 aatcaccgtc tgccggcgcg caaagcctcc ccatcatgcc gccggcatgt caaaacacgg
     4861 tcactctcgc gcgctctgaa agcgccgaga atccgactca aacaaaaagg ccttgcgatt
     4921 ccggtgaacc gcaaggcctg gaaattcttc ggaatctcaa ttcagcccta cggttcacct
     4981 cggcaattaa tgcccttgac cggcgtaccg caaaataccg ccccctgggc atggcgagcg
     5041 cgcggtccgc acgcgtccgc ctgcatggtc caagactggc tgtatctggc gatcatcgct
     5101 ggactccctt cgaatcccgg caaccattgc caggagacta tcagtatata ccacatttcg
     5161 gcaaagtcaa gacccgactc aaccccagcg ctgggtaggg caacgcacag ggaatgaaga
     5221 attgtgagtg gagaattgag aatgggccgt gttttgccgt cgcccgtgaa cccaccgtcg
     5281 gtgagctccg gagacagcgt tgattcggca caggacgtcc aatccacaag ggctttccta
     5341 aggcggaccg ccttcttgcg ggccgcaacc caagtggtct gcgatccaga aacatgcgcg
     5401 caaaatgcgc accagtcgcg acggaaggga ctaaacatgt gtggcgatgt acctgcaatt
     5461 gtagggcaga tataggccgc tcctttcctc aactatccat ctcagcccct attgcccgac
     5521 gacaatctcc agcggctggc cggtggtggc cgggtgcgtc acatggatcg tgtcgacga
//
LOCUS       DAOTPW010000314         5563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12305_length_5563_cov_5.638889, whole genome shotgun sequence.
ACCESSION   DAOTPW010000314 DAOTPW010000000
VERSION     DAOTPW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12305_length_5563_cov_5.638889"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2979
                     /locus_tag="P5316_19820"
     CDS             <1..2979
                     /locus_tag="P5316_19820"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRR87265.1"
                     /translation="SRITDVDRSKLVWSSGVVKSSQSTHVVYAGKPPVSGQRYYWRVR
                     TWDADGVASQWSPPAWWEMALLKPEDWSAKWIISQRPADVDAALKWGDWIWNAKAVEE
                     DRTIFVRKSFILDEADPVKSARIKIAADDNFTLFVNGQRVGNSSSWQVARVYELEKYL
                     RAGKNVMAIQAHNGPGQCGLLFAAQVTLNSGRMMELRSDAQCKTAQTEQPNWTSPDFD
                     DAAWERTIVVAKYGDEPWKEIGEAPPPRHAVCMRKEFTVKGQIARARAYVSGLGIYEL
                     RLNGRKVGSDVFTPGWTHYFKRVQYQTYDVTGMLKPGPNAVGAMLGNGWWSGGLGWKG
                     SDQYSQGDLRLICQIVIEYADGSRETVVTDPTWQSYMSPISRNTYYHGETYDARLEQP
                     GWDTPGFDAGKWWKTAVLEEKSAMLVAQRDETIQITEEIPPMQISEPQKGVFIFDFGQ
                     NAAGVARLKVEGAQPGAEIRLRFGEELDPNGRLYRDNYRSAEATDYYICKGGGVEVWE
                     PIFTYRGFRYCEVTGLPKPPTKATLTHRVLHTAVPPAGSFRCSNWLINRILKNVEWGL
                     RSNLHSVPTDCPQRDERLGWMGDAQAFAHTSCFLRHMGAFYTKWMIDITDSQGADGAT
                     TDVSPAKVVTDAAKPGWGDAIVIIPYTVWRFYGDTRIIEENYKGMAAWVEYMKTHGKD
                     GLYERAGYGDWVPVEKSPTEWIGSAYYFYCSKLMSEMAAAIGKTDDAWKYHDQAEAIA
                     KAFNDKHLNKQTNNYLTGTQTCNILPLYFGITPPDRTQAVLNNIIKDIMARGDHLSTG
                     FLGTTYLMALLHETGHNDLAWRLAVQTSYPSWGHMILKGATTIWERWDTDKQGPDMNS
                     RNHFAFGTVARWFYEAAAGINLDPQVPGFKRFIVRPVVVGDLTWAKATYPSMYGQIRS
                     EWKRTDKGLTLEVTIPANTTAKVYVPLLRMTRFSVTESGVPVLTPQSHVPGVRFVQLQ
                     DDVAVLDVAAGRYRFVVEAR"
     gene            3144..4373
                     /locus_tag="P5316_19825"
     CDS             3144..4373
                     /locus_tag="P5316_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HRR87266.1"
                     /translation="MDTTILLVFGFVYVGMILGRFPGLAMDRTGIALIGAIILLAGER
                     IELTDAWRAVDVPTIGLLFGLMVLSAQFRLGGMYTAITRRMVAAGISPPALLAVVIAI
                     SGLLSALLANDIVCLAVAPLLVESCARRRLNPLPFLLGLACASNIGSAATLIGNPQNM
                     LIGQKLRMSFGGYLLDGLVPAVLGLAAAWAILVRQTRGRWQGEVRLQHVEAPRFNPWQ
                     SGKGVVILIALVVAFLAAPIPRELLALAAAGIILTSRKMHSRRMLGLVDWELLVLFIA
                     LFIVNEALESTGALTLVIERLNTMHVDLGNMGWLFGITAVLSNLVSNVPATMLLLPAA
                     STHGDPHLAGSVLALSSTLAGNLFIAGSIANIIVVNQAERLGVHIGWREHARAGVPVT
                     LATLLIAAGWLALRSLM"
     gene            4469..5383
                     /locus_tag="P5316_19830"
     CDS             4469..5383
                     /locus_tag="P5316_19830"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRR87267.1"
                     /translation="MRHRRILTELLIYAAVRLAAMVFQMFPVNWNLATARRMVPIWCR
                     IMPRHLNRAREHLRLAYGSQLSAKEIDRIAFHSLEQMSMMAMECLFTPRLINELTWHR
                     YIRLNGIDKALDRLLRGQGAILVTGHYGSWELVGFALAALGFPLTAVMRPLDNPWLNE
                     FILGIRAKRGLHLLYKKGASRSAGDVLECGGLLGFIADQNAGRKGEFVDFFGRKASTY
                     KSIGLLAIEYKVPILVGYARRLSNRFEYEVGCNRIIEPHEWADKADKLHWVTQEYTSA
                     IERFVRQAPEQYLWIHRRWKTRPKDEQA"
BASE COUNT         1065 a         1640 c         1795 g         1063 t
ORIGIN      
        1 tcccgaatca cggacgtgga tcggagcaag ctcgtctgga gcagcggcgt cgttaaatcc
       61 agtcagtcga cgcacgtggt ttacgcgggc aagccgccgg tcagtgggca gcggtactat
      121 tggcgtgtgc gaacgtggga tgccgacggc gtagcttcgc agtggtcccc cccagcctgg
      181 tgggaaatgg ccttgctgaa acccgaggac tggtcggcca agtggattat cagccagagg
      241 cctgccgatg tcgacgccgc cttgaaatgg ggcgactgga tctggaatgc caaggcggtg
      301 gaggaagaca gaaccatttt tgtgcgcaag tcgttcattc tggacgaagc cgacccggtc
      361 aagagcgcgc ggatcaagat cgcggccgac gacaatttca ccctgttcgt caatggccag
      421 cgggtgggca acagctccag ttggcaagtg gcccgtgtgt acgaacttga gaagtacctc
      481 agggcgggca agaacgtgat ggcgatacag gcccacaatg gtccgggcca atgcggtctt
      541 ctttttgccg ctcaggtcac gctcaacagc ggcaggatga tggaactccg cagcgatgcg
      601 cagtgcaaga cggcccaaac cgaacaaccc aactggactt cgcccgattt cgacgatgcg
      661 gcgtgggagc gcaccattgt cgtggccaag tacggcgatg agccgtggaa ggagatcggc
      721 gaggctccgc cgcctcgcca cgcggtgtgt atgcggaagg aattcacggt caaggggcag
      781 atcgcgcggg ctcgggcgta cgtgtcaggg ctgggtatct acgaattgcg actcaatggc
      841 cggaaggtcg gttcggacgt gttcaccccc ggctggacgc attacttcaa gcgggtccag
      901 taccagacct acgacgtaac cgggatgctc aaaccgggcc ccaatgccgt cggggcgatg
      961 ctgggcaacg gctggtggag cggcggactg ggatggaaag ggtcggacca gtactcccag
     1021 ggcgatctgc gactgatctg ccagatcgtc atcgaatatg ccgacggcag ccgcgagacg
     1081 gtcgtcaccg atcccacgtg gcaatcatac atgtcgccga tcagccgcaa cacctactat
     1141 cacggcgaga cctatgatgc ccggttggaa cagcccggat gggacacacc cggtttcgac
     1201 gccggcaagt ggtggaagac ggccgttctc gaagagaagt cggcgatgct ggttgcccaa
     1261 cgcgatgaga cgattcagat caccgaagaa ataccgccga tgcagatcag cgagccacag
     1321 aagggcgtgt tcatcttcga tttcggccaa aacgcggccg gtgtggcgag gctcaaggtt
     1381 gaaggcgccc aaccgggagc cgagatccgt ctgagatttg gcgaggaact cgatccgaac
     1441 gggcggcttt accgcgacaa ttaccgttcg gcggaggcca ccgattacta catctgcaag
     1501 ggcggcgggg tggaggtctg ggagccgatc ttcacttacc gtggtttccg ctactgcgag
     1561 gtaaccggcc tgcccaagcc acccacaaaa gccacgctga ctcatcgcgt cctgcacacg
     1621 gccgtgcctc cggccggcag cttccggtgc tcgaactggc tgattaaccg catcctgaaa
     1681 aacgtagaat ggggcttgcg ctccaatctc cacagcgtgc ccacggactg cccgcagcgc
     1741 gatgagcggc tcggctggat gggtgacgca caggccttcg cccatacctc gtgctttctg
     1801 cggcacatgg gtgcgtttta cacgaagtgg atgatcgaca tcaccgactc acagggagcc
     1861 gacggagcga ccaccgatgt ctcgcccgcc aaagtggtga ccgacgccgc caagccaggc
     1921 tggggcgatg ccattgtgat catcccctac accgtctggc gattctacgg cgacacacga
     1981 atcatcgaag aaaactacaa gggcatggcc gcgtgggtcg agtacatgaa gacccacggc
     2041 aaggatggtc tgtacgagag agccggatat ggggactggg ttccggtcga gaagtcaccc
     2101 accgagtgga tcggctcggc ctactacttc tactgcagca agctgatgtc agagatggcc
     2161 gccgcgatcg gcaagaccga cgatgcatgg aaataccacg accaagcgga ggccatcgcc
     2221 aaggctttca acgacaaaca cctgaacaaa cagaccaaca actacctgac gggtacccag
     2281 acgtgcaaca ttctgccgct gtatttcggc ataacgccgc cggatcggac ccaggcggtg
     2341 ttaaacaaca tcatcaagga catcatggcc cgcggcgatc atctgtcgac cgggttcctg
     2401 gggacgacgt acctgatggc cctgctgcat gaaacggggc acaatgacct ggcctggcga
     2461 ctggccgtgc agaccagcta cccgtcctgg ggtcacatga ttctcaaagg ggcgacgacc
     2521 atctgggaac ggtgggatac ggacaagcag gggccggaca tgaactctcg caaccacttc
     2581 gccttcggta ctgttgcgag gtggttttat gaggcggcgg cgggaatcaa tctcgacccg
     2641 caggttccgg ggttcaagcg cttcatcgtc cggccggtcg ttgtgggcga cctgacgtgg
     2701 gccaaggcca cgtacccgtc gatgtacggc cagatccgca gcgagtggaa gcggaccgac
     2761 aaggggttga ccttagaagt gaccattccg gccaacacca cggcgaaggt gtacgttccg
     2821 ctgcttcgca tgacgaggtt ttcggtcacg gagagcggag tccccgttct gacgccgcag
     2881 tcccacgtac ccggtgtgcg gttcgtccaa ttgcaggacg atgttgccgt gctcgatgtc
     2941 gccgcggggc gataccggtt tgtcgttgag gcgaggtaaa gagaaccaca aggggtgccc
     3001 taccccattt ccaggcgggt gagcgatgat cgtggcgagc aacgggcacg cgaccagatc
     3061 aacataccgc agcgtccgag gccggaacga ttcgccggaa aagcgggggc gggaaaggat
     3121 tcctgcgcca gcagaccgct gacatggaca cgactatcct tctcgttttt gggtttgtgt
     3181 atgttggcat gatcctcggg cggtttcccg gtctggccat ggatcggacg ggcatcgccc
     3241 tcattggcgc gatcatcctg ttggccggtg agcggatcga attgacggac gcgtggcgcg
     3301 ccgttgacgt gccgacgatc ggcctgttgt ttgggttgat ggtgttatcc gcccagtttc
     3361 gtctcggcgg aatgtacacg gcgatcactc ggcgcatggt tgcggccggg atttcaccgc
     3421 cggcgttgtt ggctgtcgtc attgccattt ccggtttgct gtcggccctg ctggccaacg
     3481 atatcgtttg cctggccgtc gctccgctgc tggtcgagag ctgtgcccga cggcggctca
     3541 accctctgcc gtttctgctg ggattggcgt gcgcctcgaa catcgggtca gcggccacgc
     3601 tgatcggcaa tccgcagaac atgctcatcg ggcagaaact gcggatgtcg ttcggcggct
     3661 atctgcttga cggcctcgtt ccagcggtgt tgggtttggc cgctgcatgg gccatcctgg
     3721 tccggcagac ccgcggccga tggcaaggcg aagtgcggtt gcagcacgtg gaggcgccgc
     3781 gattcaaccc gtggcaatcc ggcaaaggtg tggtcatcct gattgctctt gtcgttgctt
     3841 ttctggcggc gccgatcccc cgcgaactgc ttgcgctagc cgcggcgggg attatcctga
     3901 ccagccgcaa gatgcacagc cgacgaatgc tcggcctggt ggactgggag ttgcttgtcc
     3961 tgtttattgc tctgttcatt gtcaacgagg cgttggagtc gaccggggcc ctgaccttgg
     4021 tcattgagcg actcaacacc atgcacgtgg accttggcaa catgggctgg ttgttcggga
     4081 tcacggcggt tttgtcgaac ctcgtctcca acgtaccggc gacgatgctc ctgctcccgg
     4141 ccgcctcgac ccacggtgat cctcatctgg ccgggtcagt gctcgccctg tcgagcacgc
     4201 tggccggcaa cctgttcatc gccggcagca tcgccaatat catcgtggtg aaccaggccg
     4261 agcggctggg cgttcacatc ggttggcgcg agcacgcccg cgccggcgta ccggtcacgc
     4321 tggccacgct gctgatcgcc gcaggatggc tggcgctgcg gtcgctcatg taggatcatc
     4381 acaggggaca cggtggcgcc ggccacggcg agcatccgcc ggtgccggct ttcgccaata
     4441 cgccggtgcc gggttacaat gccaccgcat gcggcaccgc cgaattctga cagagttgct
     4501 catctacgcg gccgtgcggc tcgcggcgat ggtattccag atgtttcccg tgaactggaa
     4561 tctggccact gcccggcgca tggttcccat ctggtgccgg atcatgccgc ggcacctgaa
     4621 tcgggccagg gagcacctgc ggctggccta cggctcgcag ttgtctgcca aggagatcga
     4681 ccggatcgcg ttccactcgc ttgagcagat gtccatgatg gccatggagt gcctgttcac
     4741 cccgcggctg atcaacgaac tgacctggca ccggtacatc cgcctcaacg gcatcgataa
     4801 agcccttgac cggctgctca gggggcaggg ggcaatcttg gtcaccggcc actatgggag
     4861 ctgggaactg gtgggttttg ccttggccgc gttagggttt ccgctgacgg cggtcatgcg
     4921 gccgttggac aatccctggc tgaatgagtt catcttgggc atccgggcca agcgcggctt
     4981 gcacttgctg tacaagaaag gggcatcgcg atcggccggt gatgtgctcg aatgcggcgg
     5041 cctgctgggc ttcatcgccg accagaacgc cggccgcaag ggcgagttcg tcgacttctt
     5101 cggacggaaa gcgtcaactt acaaatctat tggcctgctg gccattgagt acaaggttcc
     5161 gattctggtc ggctacgctc ggcgactgag caacaggttt gagtacgagg tcggctgcaa
     5221 ccggatcatc gagcctcacg aatgggcgga caaggctgat aagctccact gggtcaccca
     5281 ggagtacacc agtgcgattg agcggttcgt ccgccaagcg cccgagcaat acctctggat
     5341 ccaccgccgc tggaaaacac ggccgaagga cgagcaggcc tgacaagccc ggccagggcg
     5401 ttgctccctt gccttgacac cttccccgac cccgcccgtt catcttgggg cctattgtct
     5461 gattgttgcc aagtctgtgt ccttccggcc aaggacatgg gcgggattgg gaggttgggc
     5521 gaggtgttcg gggtggaacg gatgacggga aactggccac ggt
//
LOCUS       DAOTPW010000315         5515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12430_length_5515_cov_4.944139, whole genome shotgun sequence.
ACCESSION   DAOTPW010000315 DAOTPW010000000
VERSION     DAOTPW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12430_length_5515_cov_4.944139"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="P5316_19835"
     CDS             complement(<1..621)
                     /locus_tag="P5316_19835"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRR87268.1"
                     /translation="MSRRKRQKRRTPPGSPPGTLVADPTAPKPVITVIAYGSDGCSEQ
                     AIRDVADVQAYLARWPVTWINVDGLGDVQTIARLADILGLHRLAVEDTINTHQRAKVE
                     HYPNHLFMVAHMATYNDHLDTEQLSLYLGRNFVVTFQGGQPGDPFDPVRERIRRAVGH
                     VRQSGADHLAYCLIDAVIDGYFPVLETTGERLEVLEARIREQRMNPE"
     gene            complement(664..738)
                     /locus_tag="P5316_19840"
     tRNA            complement(664..738)
                     /locus_tag="P5316_19840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(701..703),aa:Pro,seq:tgg)
     gene            complement(988..3651)
                     /locus_tag="P5316_19845"
     CDS             complement(988..3651)
                     /locus_tag="P5316_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87269.1"
                     /translation="MRNSVPLLVPAIVVVFTGSSLGYVGVNDSCPWPTPYPPAGAGWA
                     SVNNPGFEIGFTNGVANQWIGWKDAGLPGQVHYDGTDRVYEGSHSQKLVMPTPTIDYQ
                     EAGIYQRIYVVPGETYTATVRVYLQIDNPDGWDLLAFLGLDPYGLDSGDSSSTTWNEV
                     SIYGQWRTATVTVQAVLPVMTISLKATRKWPRHRNTTVWFDAVTFTGPVPTDPPPSSS
                     DQDPVDPESLIPATIGPNLVTNPSFEDTYSGGVSNGWNKWWTAGSGEWKRSQRVGILG
                     GARYDCGGEQENVDMRPKVCLLAAGNPEEDPNNNATLGTSDYYKTFDHMQDTIIIGRP
                     MVDSNYGYYYANPTYYGARLAEHCKNYEQRFPRIDAWLDWNEPDTSIQWQKVLDFSYA
                     FTQRAHELGLKTVVLNLATGNPGNIWQMVNEGYDPHCGELLAIADYLGHHVYGGPTDH
                     LMVTNQNLDDACSFALRPRRFKDMYDRRGWRFPPVIATEGSTWVPWHGIYTPDQVLND
                     LTAMGGYMNADNWWCGYTNFVVGAMCSWPGFELVGQYVTGGQSMAQAIGAWNYEHPAD
                     AKDGYYSQMFGAGNVHPKNLSELTPAGLFNGGINRTVSGLINGEAYLLVAWVKYEFRG
                     MQPTQLKFYLGVDPTGQTSNGNAATIDWGVDQVADKAKVHETFTHVWRTFTAAGPTAS
                     IWLRANHPTSNPSFKFYVDLVEVRQLDDAPPSPSIELIPWQLNHTIGCGEQLTDDVFM
                     IRNSGATGTITYSLQPNCSWVSVKPDSGTSSGEQDPITVSYNTAALDPGLNTCTVTVT
                     APEASNSGTDLVINVTVEPPASDFDEDCDVDQDDFGHLQSCLTGGGVTQIPDACMNAD
                     LDGDGDVDVADFAIFQSCFSGPDLPPPSTCP"
     gene            complement(4173..5057)
                     /locus_tag="P5316_19850"
     CDS             complement(4173..5057)
                     /locus_tag="P5316_19850"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRR87270.1"
                     /translation="MPPSPVAMEPEQVVKIMLGKIGDIATLPEVTAQIIAAVDDPKST
                     AHDLHNIIKNDPALATKILKVVNSAFYGLPGQVSDLDRAIVLLGLSAVKNIAISASIS
                     RLFTAEKISDQFSARDIWRHSVGVAVATRQFCTMIGRKSSAEEAFLAGLIHDLGILIE
                     RQANPEQLAEIIGTAGKREQAFHVIETEILGVDHQILGAALAAKWKFPRGLQTVMGYH
                     HRIDVLSAENRLLPVIVHIADTLCCHDSVGFYLTAADQKIDEGLLQSVGLSEADFMTV
                     RESLMEQVEQAETILMGG"
BASE COUNT         1041 a         1643 c         1746 g         1085 t
ORIGIN      
        1 ttcgggattc atacgctgct cgcggatgcg cgcttcgagc acttcgaggc gctcgccggt
       61 cgtctccagc acggggaagt acccatcgat cacggcgtcg atcaggcaat acgcgaggtg
      121 atcggcaccg gattgccgta cgtgtccgac ggcgcgccgg attcgctcgc gaacggggtc
      181 aaacggatcg cccggctggc ccccctggaa tgtgacgacg aagttcctgc ccagatacag
      241 gctcaactgc tcggtgtcca gatgatcgtt ataggtggcc atgtgggcaa ccatgaacag
      301 atggttgggg tagtgctcca ccttggcccg ctggtgggtg ttgatggtgt cttcaacggc
      361 cagtcggtgc aggccaagaa tgtcagcgag gcgagcgatg gtctgcacgt cgcccagtcc
      421 gtcgacattg atccaggtga ccggccagcg ggcaaggtac gcctgcacgt cggcaacatc
      481 tcgaatcgcc tgctcgctac atccatcgct gccgtaggca atgacggtga ttaccggctt
      541 gggtgcggtc gggtcggcca ccaaggtgcc gggcggcgac cccggcggag ttcgacgctt
      601 ctggcgtttt cggcgggaca cgttatgcac ctcgcaggct tgcgacctgc tctacacgtg
      661 cactcggggc gactggattt gaaccagcga cctcttggac ccaaaccaag cgctctatcc
      721 aggctgagct acgccccgaa cacggccgcg agcggtgatg agcctgcggc gtgcatacta
      781 ttagacgcca ttgaagggta caggtcaatc cggggcccgg actgcgagac cctcaggctc
      841 ttcgactgtg aggccgttca gaaaagggcg caagcaggcg cgatatactg ccgcaacgag
      901 cgttctcgtt catggttcaa cgcgcagaat gcggaaccga cagacggtcc ccggtgtccc
      961 accattcccg gccccccggc cttgttttca tgggcaagtg cttggcggcg gaaggtcagg
     1021 gccgctgaaa cagctctgga agatggcgaa atcggcgaca tcgacgtctc catcgccgtc
     1081 aagatcggcg ttcatacatg cgtccggaat ctgtgtcacg cctccgccgg tcagacacga
     1141 ttggaggtgt ccgaaatcat cctggtcgac gtcgcagtcc tcatcgaaat cagacgccgg
     1201 cggctcgacc gtcacgttaa tgaccaggtc ggtgccggag tttgatgcct cgggggcggt
     1261 caccgtgacc gtgcaggtat tgagacccgg gtccagggct gccgtgttgt agctgaccgt
     1321 gataggatcc tgttcgcctg agctggttcc gctgtcgggc ttgaccgaaa cccaggagca
     1381 gttgggttgc aggctgtacg tgatcgtacc ggtggccccg ctgttgcgga tcatgaagac
     1441 gtcgtcagtg agctgctcgc cgcagccgat cgtgtggttg agttgccatg gaatcagttc
     1501 gattgagggg ctcggagggg cgtcgtcaag ctgtcgcact tcgacgagat cgacgtagaa
     1561 cttgaacgac ggattgctcg tcgggtggtt ggcccgcagc cagatgctgg ccgtcggacc
     1621 ggcggccgtg aatgtacgcc agacgtgggt gaaggtctcg tgcaccttgg ccttgtcggc
     1681 cacctggtcg acgccccagt cgatcgtcgc cgcattgccg ttgctcgtct ggcccgtcgg
     1741 gtcaacgccg agatagaact tcaactgtgt cggctgcatg ccgcgaaact cgtacttgac
     1801 ccacgcgacg agcaggtacg cctcgccgtt gatcaaacca ctaacggtcc ggttgatacc
     1861 accattgaac aagccggccg gtgtcagctc gctgaggttc tttggatgca cgttgcccgc
     1921 gccgaacatc tgggagtagt agccgtcctt cgcgtccgcc gggtgctcgt agttccaggc
     1981 accgattgcc tgagccatgc tttggccgcc ggtcacgtac tgaccgacca gctcgaagcc
     2041 aggccagctg cacatggcgc cgaccacgaa gttggtgtag ccgcaccacc agttgtcggc
     2101 attcatgtag ccgcccatcg cggtcaagtc attgagtacc tgatccgggg tgtagatgcc
     2161 atgccaggga acccaggttg agccttcggt ggcgatgacc ggcgggaacc gccacccgcg
     2221 gcgatcgtac atgtccttga aacgacgagg acgcaaggca aagctgcagg cgtcgtccag
     2281 gttctggtta gtgaccataa gatgatcggt cggtccgccg tagacgtgat gacccaggta
     2341 atcggcgatc gccagcaact cgccgcagtg cggatcgtag ccctcgttga ccatttgcca
     2401 gatgttgccc gggtttccgg tcgcgaggtt aagcacgacg gtcttgagac ccagctcgtg
     2461 ggcccgctga gtaaatgcgt aggagaagtc taacactttt tgccactgga tagacgtgtc
     2521 gggctcgttc cagtccagcc aggcgtcgat gcgggggaac ctttgctcat agttcttgca
     2581 gtgttccgcg agccgggcgc catagtatgt ggggttggcg tagtagtagc cataattgga
     2641 atcgaccatc ggccggccga tgatgatggt atcctgcatg tggtcgaagg ttttgtagta
     2701 atcggaggtg cccagcgttg cgttgttgtt agggtcctcc tccggattgc ccgcggcaag
     2761 caggcagacc ttgggccgca tgtccacgtt ctcctgctct ccgccgcaat cgtatctggc
     2821 cccgccaagt atgcctaccc gctgagagcg tttccactcg cccgagccgg ccgtccacca
     2881 tttgttccac ccgttggaga caccgccgga ataggtgtcc tcgaagcttg ggttcgtgac
     2941 aaggttgggg ccgatggtgg ccggaatcag actctcgggg tcgacggggt cctggtcgga
     3001 tgaagatggt ggcgggtcgg tcggcaccgg accggtgaag gtcacggcgt caaaccacac
     3061 cgtggtgttg cgatgccgag gccacttccg agtggccttc agtgagatgg tcatcaccgg
     3121 cagcacggcc tggaccgtca ccgtggccgt gcgccactgc ccgtagatcg atacttcgtt
     3181 ccaggtggtg gagctgctgt cgccgctgtc caacccgtag ggatcgagcc ccagaaaagc
     3241 caggaggtcc caaccgtcgg gattgtcgat ctggagataa actcgaaccg tagcagtgta
     3301 ggtctctccg gggaccacgt agatgcgctg gtagatgccc gcctcctggt agtcgatcgt
     3361 tggtgtcggc atgaccagct tctgcgagtg gctgccttcg tagactcggt ccgtgccgtc
     3421 gtaatgaacc tggcccggca ggccggcatc cttccagccg atccattggt tggcgacgcc
     3481 gttggtgaag ccgatctcaa aaccgggatt gttcacggat gcccagcccg caccggccgg
     3541 tggatacggc gtcggccagg ggcacgaatc gttcacgccc acgtacccga gcgacgaacc
     3601 tgtgaatacg acgacgattg ccggaacaag cagcgggacc gaattgcgca tcagcttgac
     3661 cctcaacgaa gacgcccgaa agaagccgac tccgcgacga atctcatcgt accgtcaacg
     3721 cagcggccac gcaaggggaa tatgggaaaa gaggtagctg gaaagcatca cggacctggc
     3781 cacctaccgc aaggacggca tctactctct ggtcatgcaa gtggccgaag cccccataag
     3841 acccgtgggt caacgacgac caagaacata tcctacccct acgcacgacg tccgccggtc
     3901 tgctcggcgg aaggcccgcg agcccgaaaa gccgaccggg acgaatgagt agatgactga
     3961 gcacgattga tcttcgctgg ggacggaacg taccgggctc agaactcgat caccaagcct
     4021 tgccgcagtg gggtatcgtg agatggcgcg atcatgttcg gtcccgcggc cgttggggca
     4081 aatcctgccc gcagcagcat ctcatttgtc ggatcagaat atcgcctgtc ggaatgcttc
     4141 gcggaatcgg ccgataccga ggcagtcatc cctcagccgc ccatcaagat ggtttcggcc
     4201 tgttcgacct gctccatgag gctctcgcgg acggtcatga aatccgcctc tgaaagcccg
     4261 actgactgca agagtccttc gtcgattttc tggtcggcgg ccgtgaggta gaaaccgacg
     4321 ctgtcatggc aacagagcgt gtcggcgatg tgaacaataa ccggcagcag gcggttctcg
     4381 gcgctgagga cgtcgatgcg gtggtgatag cccatgactg tctggaggcc ccgcgggaac
     4441 ttccacttgg cggccagggc ggctccgagg atctgatgat ccacgcccag gatctcggtc
     4501 tcgatcacgt gaaatgcctg ttctcgcttg ccggcagtac caatgatctc ggccaactgc
     4561 tcgggattgg cctgtcgttc gatgagaatg ccaaggtcgt ggatcaagcc ggcgagaaac
     4621 gcctcttcgg cagaggattt cctgccgatc atcgtgcaaa actggcgggt ggccacggcg
     4681 acgcccacgc tgtgtcgcca gatatcgcgg gcgctgaatt gatcgctgat cttctcggcg
     4741 gtgaacaggc ggctgatcga ggccgaaatg gcgatattct taaccgcgct gagtcccagc
     4801 aaaacgatgg ccctgtccag atcggatacc tggcccggga ggccatagaa cgccgagttg
     4861 acaaccttga ggattttcgt ggccagcgcc ggatcgttct tgatgatatt atgaagatcg
     4921 tgagcggtgc tcttgggatc gtccaccgcc gcgatgatct gggccgtcac ctcgggaagt
     4981 gtggcgatgt ccccgatttt tccgagcatg atcttgacga cctgctcggg ttccatggcg
     5041 accggtgaag gtggcatcag atcgcgtcct tttcagacta aggcggaccg caaccaaggc
     5101 tcgccgtggt ccagaaacat gcgtgcaaaa cgcacatcag tcacgacggc cgagaccgat
     5161 gttctggacg ggcgtttgcc cgcccggtca tcatcgtcgc tcggacgtcc gggctttagc
     5221 tgcaaaccgg ccctggccac tcgaaaatcg ggccttgcag gtggcgatcg cgactctacc
     5281 tggcacggat gcgagactgc gcccaccggc agcactcact tgttccggtt aggccggcgg
     5341 cacggtctct cgaccttgcc aatgcaaaag ttagccttgt ccgccgctgg tgcgggaacc
     5401 catcttcaac cctcctgggg gggccggtaa cgcaccggca tgccggcttc gcgacgcggc
     5461 cgggggcccc acgccggtcg ggcggccgcg gaatcggcaa aggcgcagac tggaa
//
LOCUS       DAOTPW010000316         5460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12584_length_5460_cov_4.750416, whole genome shotgun sequence.
ACCESSION   DAOTPW010000316 DAOTPW010000000
VERSION     DAOTPW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12584_length_5460_cov_4.750416"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="P5316_19855"
     CDS             <1..732
                     /locus_tag="P5316_19855"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR87271.1"
                     /translation="PTLTPGPAKFNNPTAEAQYRAAREAGHFIAITPAEPMWFVIENT
                     MGFEDGLLAMASEPELVADMVATYADYVLNMLVLTLQRGYRFDAIWFWSDLCYRNGML
                     FSPADARRFVLPHWKRFGEFACRHGMRFMFHCDGNVAELIPLLIEAGCHAIQPIEARA
                     GNDVRDYKQRFGRHICFIGNINADVVASGDFEQIEREVTDKIPLAAADGGYIYHIDHS
                     VPPTISLAAYRHLLDCVRRCQVPHA"
     gene            complement(687..2969)
                     /locus_tag="P5316_19860"
     CDS             complement(687..2969)
                     /locus_tag="P5316_19860"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRR87272.1"
                     /translation="MSNDWSIIESPFHVETAKAYEGLFTLGSGYLHVRGSLEEHLAGA
                     PQNVSYTRLPANVTSERFADTKSKWGTYVPGVFGRHPLLNNEMINLPFFLGLAPIVDD
                     ERLDMESAQITGYRRELRLDTATLIRTLCWRTRNHKQVELTFERFVSAARPYLCVQRL
                     RFRCDREARLDLHLGIDADVRTNGYDHFKTIELRPTSGLGCECHVITDAGDEVLVATR
                     LVYHLPRDGNCELSVRHEQAERACAVVAEATIKEPVGRELVFEKRSAVTTSRDPVRSD
                     ADGWLDIAADQSYEQLHAEHVAIWKRRWDSCDVVIEGDEDSQRAVRLSLYHLLRAHVP
                     DDPRVAVDAKGYAGEAYWGRYFWDTEMFLLPFYLYTNPVRARTLVDFRVQSLAGAKEN
                     ANRYGYPGARSAWESDAEGRECCPNWQYADHEVHVTADVVYGLAHYAHATGNMDYLRG
                     PAAEVILETARYWMARLDWREGDGYPSLLGVMGPDEYTPISNNNSYTNRMVAFALKTA
                     AEIGPCAGASPSECEAFAAAAASLPIPRDKDGLLVLQCEGFDRLADARCDELWRDRDT
                     TFAAQVSQERLYRCKCLKQADVLMLMMLFPGEFSDAEVRRAWDYYLPLTTHDSSLSAG
                     VHAIVATRLKLMDEAWRFWMRARAIDLDVEHGGAAEGIHIANAGAVWQMLVLGFAGMR
                     TAMQAEMLTLMPSLPAAWSRLAFPVVWQGQPVYVEIEPEEVRIANRGSAPLEVQIHDD
                     RRVIAAQACGTWQRRTQSKR"
     gene            complement(3052..4797)
                     /locus_tag="P5316_19865"
     CDS             complement(3052..4797)
                     /locus_tag="P5316_19865"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRR87273.1"
                     /translation="MTAKIVLGAALAAACARAELPPPPPFSPAGVPAVSTAEPAANPS
                     STADLDVTYIERTPRYDYDAAKNNPAPGDSVTFRGHIRNTGDSPAASVDYEWRLDDVP
                     VASGTLTNLAVNEERVVTWGWVWQDGNHWIKLQVDPNNVIGESSEVNNEVVDRTDGII
                     VGFWVEQSVYDYFHQYQKDLGVGSNSWDDWAQRQMRFWNQFSQNAIYPTTPNGVLDRV
                     RIDKIVVVPDGALPLAGGYPTNHPDLRDKTVDLMWGFPATLLNGSMYSNHTSASISNP
                     FYYEGSLLHELGHARYLIDTYGFDVHNTSSHHSVQIYEGSVYVAGSSYMPFLAWDEVL
                     YYNKYGKMMSGGYDQGWSEYDAGALNRIAGQRACCGNYNAPYNIGAFLNDLPANNHLR
                     ITDEAGQPRRGANVRIYQAVSGPGWYGKTIDNTPDLEFTTDNAGYVLLPRNPFTAGSI
                     QHNYGIANGIVVVRIAHLDQIWYRFQEVTDFNIQYWRGNTQDAYYTIALPGPNETPVS
                     ADFDGDGDVDQEDFGHFQICLTGSGGAIAPGCEDAILDGDTDVDAADLSIFTSCISGA
                     DVPPADPTCLDLGSL"
     gene            complement(4917..>5460)
                     /locus_tag="P5316_19870"
     CDS             complement(4917..>5460)
                     /locus_tag="P5316_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87274.1"
                     /translation="PLAAGAWQHIEYRFDFGAATPTVSVSVNGVSASASLGETETSIR
                     GWDFEVEGTLKDGDFEATPITPIFSYDTGTAEMISGLQMGPVAGGATQQIYYWVGDTL
                     TALTYVKPIPGDFNKDGYVDENDVTIFEGCATGPGVPYTDPNCNPADLDPDGDIDHAD
                     FGLFQRCISGTAPGDVNCLD"
BASE COUNT         1027 a         1752 c         1634 g         1047 t
ORIGIN      
        1 ccgacgctga cgcccggccc tgcaaagttc aacaacccga cggccgaagc tcagtaccgg
       61 gcggcgcggg aagccggcca cttcatcgcc atcaccccgg ccgaaccgat gtggttcgtt
      121 atcgagaaca ccatgggctt cgaggacggg ctcctggcga tggcctccga gcccgaattg
      181 gtggccgaca tggtggccac ttacgccgac tatgtgctga acatgctggt cctcacgctc
      241 cagcgcggct accggtttga cgcgatatgg ttctggagcg acctgtgtta ccgcaacggg
      301 atgctgttct caccggcaga cgcgcgccgg ttcgtcctgc ctcattggaa acgcttcggc
      361 gagttcgcct gccggcatgg gatgcggttc atgtttcact gtgatggtaa cgtcgccgaa
      421 ttgatcccgt tgctcatcga agccgggtgt cacgcgatcc agccgatcga ggccagggca
      481 ggcaacgacg tgcgcgacta caagcagcgc ttcggacggc acatctgctt tatcggtaac
      541 atcaacgctg acgtcgtggc ctcgggcgac ttcgagcaga tcgaacgcga ggtcaccgac
      601 aagatccccc tcgccgccgc ggacggaggg tacatctacc acattgacca ctccgtaccc
      661 cccacgatct ctctggccgc gtaccgtcac ctcttggact gcgtgcggcg ttgccaagta
      721 ccgcacgctt gagcggcaat cactcgacga tcgtcgtgta tctgaacctc cagcggggcc
      781 gatccgcggt tggcaatcct gacctcctca ggttcgatct ccacgtagac gggttggccc
      841 tgccagacca ccgggaatgc tagccgggac caagcggcgg gcaggctggg cataagcgta
      901 agcatttctg cctgcatggc cgtcctcatg ccggcaaaac cgagcaccag catctgccaa
      961 accgcacccg cgttggctat atgaatgccc tcggctgctc ccccatgttc cacgtcgagg
     1021 tcgatcgccc gggcacgcat ccagaatcgc catgcctcgt ccatcaattt cagccgggtc
     1081 gccacaatcg cgtggacgcc cgcggacaac gaggagtcgt gggtggttag cggcagatag
     1141 tagtcccagg cccggcggac ctcggcatcg ctgaattcgc ctggaaagag catcatcagc
     1201 atcaagacat ccgcctgttt gaggcacttg caccggtaga ggcgctcctg tgaaacctgt
     1261 gcagcaaagg tcgtgtcgcg gtcgcgccac agttcgtcgc accgggcatc ggccaggcgg
     1321 tcgaagccct cgcactggag caccagcagg ccatctttgt cccggggaat cggcagactc
     1381 gcggccgccg cagcgaacgc ctcacattcc gatggcgaag ctccagcgca aggaccgatc
     1441 tcagccgctg tcttgagcgc aaaggcaacc atccgattgg tgtagctgtt gttattggag
     1501 atcggcgtgt attcatcggg ccccatgacg cccagcaggc ttggatatcc gtcaccctcc
     1561 cgccaatcca atcgcgccat ccaataccgc gccgtttcca gaatcacctc cgcggccggg
     1621 ccacgcaggt agtccatgtt ccccgtcgca tgagcatagt gtgccaagcc atagaccacg
     1681 tcggcggtaa catgcacctc gtgatcggca tactgccagt tcggacagca ctcgcgcccc
     1741 tccgcgtccg attcccacgc ggagcgcgcg cccggatacc cgtagcgatt cgcgttttcc
     1801 ttcgcaccgg ccaggctctg cacgcggaag tcgaccagcg tgcgggcgcg aacggggttg
     1861 gtatagagat aaaacggcag cagaaacatc tccgtgtccc agaaataccg gccccaatag
     1921 gcttcgcctg catagccctt ggcgtcgacg gccacccgcg ggtcgtcagg cacgtgggcc
     1981 cgcagaagat gatagagcga aagccgcacc gcccgttgcg aatcctcatc gccttcgatg
     2041 acgacgtcac acgagtccca ccggcgcttc cagatcgcga catgctcggc gtgaagctgc
     2101 tcatatgact ggtccgcagc gatgtccagc caaccgtccg cgtcgctgcg caccggatcc
     2161 cggctggtgg tcacggcgga tcgcttctca aagaccagct cacggccgac cggttcctta
     2221 atcgtcgcct ccgccaccac ggcgcaagcc cgctctgcct gttcgtgacg gacgctcaac
     2281 tcgcagttcc cgtcccgcgg caggtggtag acaagcctcg tggcaaccag cacttcatcc
     2341 ccggcgtcgg tgataacatg acactcgcag cccagaccgc tcgtcgggcg aagctcaatc
     2401 gtcttgaagt ggtcgtagcc gttggtccgc acatcagcgt cgatccccag gtgcagatcg
     2461 agacgggcct cgcggtcgca acggaacctc agccgctgga cgcacaagta ggggcgggcc
     2521 gcgctgacga atcgctcgaa agtcagctcc acttgcttgt gattgcgggt gcgccagcac
     2581 aacgttcgaa tcagtgtcgc cgtatcgagc cgcagctctc tccgatagcc tgttatctga
     2641 gcactctcca tgtcgagccg ctcgtcgtcg acgatcggcg cgaggcccag aaagaacggc
     2701 aggttgatca tctcgttgtt aaggagcgga tgacggccga aaacgccggg gacgtatgtc
     2761 ccccacttgg acttcgtgtc ggcgaaccgt tcgctggtca cgttggccgg aagtctcgtg
     2821 tagctgacgt tttgcggcgc acccgccaag tgctcctcca gcgagccccg aacgtgcaga
     2881 tagccgcttc cgagggtgaa cagcccctca taggccttgg cggtctcgac atgaaatggt
     2941 gattcaatga tggaccagtc gttcgacatc agccacagtg taaccggacg accctctgtt
     3001 gggcaggtaa cacaaagcgt caagctcacg tgagcccggg aatgcccccc gtcagaggga
     3061 acccaggtcc aggcaggtcg gatccgctgg cggcacgtcc gccccgctga tgcagcttgt
     3121 gaagatgctc aaatccgccg catccacgtc cgtgtcgccg tccagaatgg catcctcgca
     3181 accgggagcg attgctccgc ccgagccggt caggcatatc tggaagtggc cgaagtcctc
     3241 ctggtccacg tcgccatctc cgtcaaagtc ggccgagacc ggcgtctcgt tgggccccgg
     3301 caaggcgatc gtgtaataag catcctgcgt attgccccgc cagtattgga tgttgaaatc
     3361 ggtgacctcc tgaaatcgat accagatctg gtcgaggtgg gcgatccgca cgaccacgat
     3421 gccgttggcg atgccgtagt tgtgctggat gctgccggcg gtgaagggat tgcgcggcag
     3481 gagaacgtac ccggcgttgt cggtggtgaa ttcgagatcg ggcgtattgt cgatggtctt
     3541 gccgtaccag ccggggccgc tgacggcctg gtagatccga acgttcgcgc cgcgccgggg
     3601 ttggccggcc tcgtccgtga tccgaaggtg gttgttggcc ggcaggtcgt tgagaaacgc
     3661 gccgatgttg tacggggcgt tgtagttgcc acagcaggcc ctctgcccgg caatgcggtt
     3721 gagagcccct gcatcgtact ccgaccaccc ttggtcataa ccgccggaca tcatcttgcc
     3781 gtacttgttg tagtagagga cttcgtccca cgccaggaac ggcatgtatg agctgccggc
     3841 tacgtagacg cttccctcat agatctgtac cgagtggtga ctcgacgtgt tgtgcacgtc
     3901 gaagccatag gtatcgatca ggtagcgggc atgccccaac tcatgcagga gggagccctc
     3961 ataataaaac gggttgctta tggaagcgct ggtgtggttg ctgtacatcg agccgttcag
     4021 gagcgtggcc gggaagcccc acatcaagtc caccgtcttg tcccggaggt caggatggtt
     4081 ggtgggatac ccgccggcca agggcagtgc cccatcgggt acgacaacga tcttatcgat
     4141 ccgcacccgg tccaggaccc cgttgggtgt cgttggatag atcgcgttct gggagaactg
     4201 gttccagaag cgcatctgcc gctgggccca gtcgtcccac gagttcgagc cgacgcccag
     4261 atccttctgg tactggtgga agtagtcgta aaccgactgc tccacccaga agccgacgat
     4321 gatcccgtcg gtgcgatcga ccacctcgtt gttgacctcg ctgctctcgc cgatgacgtt
     4381 gtttgggtcc acctgcagct tgatccagtg gttgccgtcc tgccagaccc atccccaggt
     4441 gaccacgcgt tcctcgttca cggccagatt ggtcagcgtg ccggacgcga cgggaacatc
     4501 atcaagccgc cactcgtaat ccacggaagc cgccgggctg tcgcccgtgt tcctgatgtg
     4561 cccacgaaaa gtgactgaat cacccggagc agggttgttc ttggccgcgt cgtagtcata
     4621 ccgtggggtg cgctcgatgt aggtcacgtc caggtctgcc gtgctcgacg gattggctgc
     4681 cggctcggcc gtcgacaccg ccggtacgcc ggcaggcgag aaaggcggtg ggggcggcag
     4741 ctccgccctg gcgcatgccg ccgctagtgc cgcaccgagc acgatttttg cggtcatcgc
     4801 gatgttgctc cctataaaac ggcttgaaac gcaagcgaaa ggtcaaacac aagaccgttt
     4861 cgcttgcgtt tcaaagcctg ctcaacagga cacgtcggtc ccagaatctt gaacaatcaa
     4921 tccaggcagt tcacgtcacc cggcgccgtc ccgctgatac acctctggaa cagcccgaag
     4981 tccgcgtgat caatgtctcc gtccggatcc agatcagcag ggttgcagtt tggatcggtg
     5041 taggggacgc ccggtccggt cgcgcaaccc tcaaagatag tgacgtcgtt ctcatcgacg
     5101 tagccatcct tgttgaaatc gcctgggatc ggcttgacat aggtcagagc cgtgagggta
     5161 tcacccaccc agtaatagat ttgctgggtc gcgccgccgg cgaccggccc catctgcaaa
     5221 ccgctgatca tctcagccgt gccggtatca tacgagaaga tcggggtgat cggtgtcgcc
     5281 tcgaagtcac catccttgag ggtgccttcg acctcgaagt cccacccgcg gatgctggtc
     5341 tccgtttcgc ccaagctggc gctcgccgag accccattta ccgagacgct caccgttggg
     5401 gtcgctgctc caaagtcgaa ccggtactcg atgtgctgcc aggcacccgc ggccaggggg
//
LOCUS       DAOTPW010000317         5456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12595_length_5456_cov_5.365858, whole genome shotgun sequence.
ACCESSION   DAOTPW010000317 DAOTPW010000000
VERSION     DAOTPW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12595_length_5456_cov_5.365858"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..643)
                     /gene="rpmB"
                     /locus_tag="P5316_19875"
     CDS             complement(374..643)
                     /gene="rpmB"
                     /locus_tag="P5316_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRR87275.1"
                     /translation="MSRICYFTGKRTRSGRQYTRRGKAKYLGGVGRKVTSKTKRKFKP
                     NVQKVRAIIDGKPVRVMVSTKAIRDGKIVKPYKRVYKPQEAKAEG"
     gene            complement(880..1995)
                     /locus_tag="P5316_19880"
     CDS             complement(880..1995)
                     /locus_tag="P5316_19880"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR87276.1"
                     /translation="MIAATAVMILFAGENLDKWITLSGRWEVREGVMTCVAAPSMIRS
                     GYESDEFILHFQYRRASGGDNRLAIHSKLTTGGTLLRLTSSGVFPVASSLASGPTYAD
                     DTWIDAVMEVVRGQMWITCRAADERDSPGKAGDPIAVSIPADSRGFLRFEAGKPGLEI
                     RNVRVEEPGFRNLFDNGTLAGWEIVRPGNPESPGWSNENGVVRCRGRGSGWLRTLETH
                     DNFVLRLEYQLPVSGNSGIYLRAPLEGRVSRIGLEVQLLDDAAFRGRFKPAQFAGSVY
                     DAIAPEIQVPAPANQWNAIEVLLDGRRVRTILNGTRLYDARLDDASKDVNSDKRPLST
                     RRLTGFIGLQEHSTAVEFRHVRLKELAGQLNRNSLSK"
     gene            2161..2439
                     /gene="xseB"
                     /locus_tag="P5316_19885"
     CDS             2161..2439
                     /gene="xseB"
                     /locus_tag="P5316_19885"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRR87277.1"
                     /translation="MPSASEPEKKGEQKEQLTFEQAYSQLESIAKAIEQGKIGLEDSI
                     VEYEKGVKLLRYCRAVLAEAELKVQKLQVADDGTLTPGPMPPPDEETA"
     gene            2470..3636
                     /gene="nifS"
                     /locus_tag="P5316_19890"
     CDS             2470..3636
                     /gene="nifS"
                     /locus_tag="P5316_19890"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HRR87278.1"
                     /translation="MSVIYLDNNATTRIAPEVLEAMLPWLGERYANPSSVHHFGQQAR
                     HAVEVAREQVAAAMGVRDRQIIFTGSGTESNNLAIRGVLAARPEKRHVATTAVEHDSV
                     LRPMQQLEREGYRVTYLGVDSLGHLDLGELEASLSDQTALVSIMHANNETGVIFPIER
                     IAALTSAKDIPLHVDAVQTTGRIRDASGHSPLNLVDVPVDLLTASAHKFHGPKGVGIL
                     YLRRGARLRPQVLGGHQERDLRAGTENVAGIVGAGRALELAMRHLDADSARVATLRDR
                     LEEGICSRVSIARVNGDRVGRIPNTTNIGFEALQAEAILMLLSNHGVCASSGSACSSG
                     SLEPSHVLTAMGIDRRVVHGAIRFSLSRFTTEAEIDQVLDLMPGIIERLQKLQR"
     gene            complement(4113..4706)
                     /locus_tag="P5316_19895"
     CDS             complement(4113..4706)
                     /locus_tag="P5316_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87279.1"
                     /translation="MAMMTHSSKVWHGVMLAGLLAGLAGCSSQSTRIHLTTYDETGRE
                     QHHYADFNRVAFLINPSGLLELGMRVEQPSKLDPTQTITQLVHIKEVWKPVPGRTAVE
                     RTQINVQVRYGILTPPTGVRYDGAGFLYARFKEGSTTISGEIESGRLAPSYRMADAML
                     PFGVARISGPFRAVKNPGEVIEIARQLDLLFSKQTER"
     gene            5060..>5456
                     /locus_tag="P5316_19900"
     CDS             5060..>5456
                     /locus_tag="P5316_19900"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRR87280.1"
                     /translation="MKPQQTDYRLGAVSFLNTKPLIEGLGDESGIRLELAVPAELAGM
                     LQAGRVDAALVPVIDIGRSAGRWKRISDAGIGSNGDTLTVQVFSRVPPEDMTTLHADT
                     HSHTSVMLARLVWKGFHRRPLAVVPLKPGT"
BASE COUNT         1048 a         1692 c         1664 g         1052 t
ORIGIN      
        1 cgttccaccc cgaacgcctc gcccaacctc cccgccccgc ccacgtcctt ggccgaaagg
       61 gtacacatat tcaaaccggc gaaatctctg cactttcaga acgacgctgg cagaaaggcc
      121 acccgtaacc gcatcctgac gccccagaaa ccagatcgga aaccaaaaca aacgtcgaaa
      181 caggtatggt tttccccgcc ctggtttggc ggttcctaac ggggcagaag atgtgattaa
      241 cgcacgacgc agatcagatc aagaatctct tccgaaacct gtgcgggacc cacttccagc
      301 cgattggctt tcggggtttc gggagaggct tcaaggttgg agaattgggt gttgtcgccg
      361 tgtgcggtgc ctgtcagcct tcagccttcg cctcctgcgg cttgtagacc cgcttgtaag
      421 gcttgacgat cttgccgtcg cggatggcct tggtgctgac catgacgcgg accggcttgc
      481 cgtcgataat cgcccgcacc ttctggacat tcggcttgaa cttgcgcttg gtcttgctgg
      541 ttaccttgcg acccacaccg cccaagtatt ttgccttccc gcgccgcgta tactgacgac
      601 cgcttcgggt ccgcttgccg gtgaagtagc atatgcgtga catctcatac ctcgccgtcg
      661 tttgtcattc caaattacga gccctgtatt gtagcagttg ccgccgagaa tacaaatgtc
      721 gacaaccagc cgaagatccc ttcgcaaaac ctgtgcggga ctggcctcca gccagtcggc
      781 tttcggggtt tcgggattgg ctttgggccg gcacgttggc gaggccttgc ggaagggccc
      841 acccgcgggt cggcgaggct tggccgcgag tcacaagtcc tattttgaca aggagttgcg
      901 attcaactgc cccgccaact ctttcagccg tacatggcgg aactcgacgg cggtggagtg
      961 ctcctgaagg ccgatgaagc cggtcagacg acgggtcgag agcggccgct tgtcgctgtt
     1021 gacgtctttc gaggcatcat caaggcgggc atcgtataac cgggttccgt tgaggatggt
     1081 gcgaacccgc cggccgtcga gcagaacctc gatcgcgttc cattggttgg ccggggctgg
     1141 aacctggatc tcgggggcga tcgcatcata gaccgacccg gcgaactgag cgggcttgaa
     1201 ccgcccacga aaggcggcgt catccagcaa ctgaacctcc aggccaattc ggctgacccg
     1261 cccttcaagg ggcgctcgca ggtagatacc gctgttcccg ctcaccggaa gctgatactc
     1321 gagcctcagg acaaagttgt cgtgcgtttc cagtgtgcgg agccagccgc ttccacggcc
     1381 acgacaacgg acgacgccgt tctcattcga ccagccgggc gattcgggat tgcccggtcg
     1441 tacgatttcc catccggcca gcgtgccgtt gtcgaacaag ttgcgaaaac ccggctcttc
     1501 gacgcggaca ttgcggatct caagccccgg cttgccggcc tcaaatcgca gaaagcctct
     1561 tgaatcagcc ggaatggaca ccgcgatcgg gtctcccgcc ttgccgggcg agtcgcgttc
     1621 atccgccgct ctgcacgtga tccacatttg ccctctgacc acctccatga cggcgtcgat
     1681 ccaggtatcg tcggcgtagg tgggcccgct ggccaaagac gaagccaccg ggaacacccc
     1741 cgacgaggtc agccgcaaca gagtgcctcc ggtggtcagc ttcgagtgga tggcaaggcg
     1801 gttgtctccc ccagacgccc gccggtactg aaaatggagg atgaattcat cagactcata
     1861 gccggagcgt atcatcgacg gcgccgccac gcaggtcatc acgccctcgc gcacttccca
     1921 ccgcccagac aacgtgatcc acttgtcgag attctctccc gcaaagagaa tcattactgc
     1981 tgttgctgct atcatgatgg gcaactctcc gcagaaaccg ccggccggct gccccggcac
     2041 cggatgcctc cggccgtggt gaattgtgtc gagacgggac gccggtgtcc atggccaagc
     2101 ccgcccggat cgcgtacaat tggccggtgg attcggacac taatcagggg atacaagcca
     2161 atgcccagcg ccagcgaacc agaaaagaag ggcgagcaga aagagcaact gacgtttgag
     2221 caggcatact cgcaacttga gtccatcgcc aaggctatcg agcagggcaa gatcggcctg
     2281 gaagactcga ttgtcgagta cgagaaaggc gtcaagctct tgcgatactg ccgggcggta
     2341 ctagccgagg ccgaactcaa ggtccaaaag ctccaggtag ccgacgatgg caccctgacg
     2401 cccggcccga tgcccccacc ggacgaggag accgcataga gcttatccgc cgcccgtcag
     2461 gaccgcgaca tgtccgtcat ttatctcgac aacaacgcaa ccacccggat cgccccggag
     2521 gtgttggagg cgatgctccc atggctgggc gagcgctacg cgaatccatc gagtgtccat
     2581 catttcggcc agcaggcccg tcatgccgtc gaggttgccc gcgagcaggt cgccgcggca
     2641 atgggtgttc gcgaccggca gatcattttc accggtagcg gcaccgagtc taataatctg
     2701 gcgatccgcg gagtcctcgc ggccaggccc gagaaacggc atgtcgcgac cacggccgtc
     2761 gagcatgatt cggtgctgcg acccatgcag cagctcgaac gagagggtta ccgtgtcacg
     2821 tatctgggtg tcgactcgct cgggcacctc gacctcggcg aattggaggc ttcgctgagc
     2881 gatcagacag ccttggtcag catcatgcac gccaacaacg agacgggggt gatcttcccg
     2941 atagagagaa tcgctgctct gacgtcagcg aaagacattc cgttgcacgt ggacgcagtc
     3001 cagacgacag gcagaatccg cgatgcgtcc gggcattcgc cgttgaacct ggtcgatgtg
     3061 ccggttgatc tgctgaccgc gtcggcgcat aagtttcacg gccccaaagg cgtaggaatt
     3121 ctttacctgc gaaggggcgc gcgactgcgg ccgcaagtgc tcggtgggca tcaggagcgc
     3181 gatctgcggg cgggtaccga gaacgtggcg gggattgtcg gtgcgggccg ggcccttgaa
     3241 ttggcgatga ggcacctgga tgccgattcc gcacgcgtgg caacccttcg agaccggctt
     3301 gaagaaggca tctgctcgcg ggtcagcatc gcgagagtca acggcgaccg ggtcggccgt
     3361 atccccaaca caaccaacat cggctttgag gccctgcagg ccgaagccat cctgatgctg
     3421 ctgagcaatc acggcgtgtg cgcctccagc ggctcagcgt gcagcagcgg ctcgctggag
     3481 ccgtcgcacg tgctgaccgc catgggtatt gaccgccggg tggtccacgg cgcgattcgg
     3541 ttcagcctgt cgcggtttac caccgaagcg gaaatcgacc aagtgctgga cctgatgccc
     3601 gggatcatcg agcggctgca gaagttgcag agatgaggtc agaagctctc agctttcaga
     3661 tctcggctgt tggcttttgg ggtccgcgct cgcagaccga tctgagatcc gaaatccgcc
     3721 aatctcaaat ctgaccgctc agccatggtg gatccaagac gtcgctgggc caagaatcct
     3781 ttctcggccc accccgtgcc agggttgcca tcccgcgata agttcgcagc gtcttgctgg
     3841 gccgagtagc gataaaaacg gctgacctcg gctctcgatg ccggaaaacg caagaaagtc
     3901 ttaccaacat cgtgagggac tgcccttcat ttgcaccacg agctactaca gtcaagttca
     3961 ctgcgctggg aggcatgaac acggcgtgct tgcgcggcca tgcgggtgtc ccaccttttt
     4021 caaggtgggg gagcgacgac ccatctggtc ctgaaggcga ttgatgctcc atcctttggg
     4081 cacggcgaaa tggggatcag cgttcatgac acctaccgct ccgtctgctt gctgaacagc
     4141 aaatcaagct gccgggcaat ctcgatgact tcgccggggt tcttcaccgc tcggaagggc
     4201 ccggagatgc gggcgacgcc gaagggcagc atggcatccg ccatgcggta gctcggggcc
     4261 agccgaccgg actcgatttc gccgctgatg gtcgttgagc cctccttgaa tctggcatat
     4321 aggaacccgg ccccgtcata gcgcacgccg gtcggcggcg tcagaatccc gtaccggacc
     4381 tgcacattga tctgcgttcg ctcgactgcg gtgcgtcccg gcaccggttt ccacacctct
     4441 ttgatatgca cgagctgcgt gatcgtctgg gtgggatcca gtttgctggg ttgttcgacg
     4501 cgcattccca gttcaagcag cccgcttggg ttgatcagga acgccacccg gttgaagtcg
     4561 gcgtagtggt gctgctcgcg gccggtctcg tcgtaggtgg tcaggtggat ccgcgtcgac
     4621 tgcgacgagc atccggccag gccggccagc aagcccgcta acatgacgcc gtgccatacc
     4681 ttgctcgaat gggtcatcat tgccatcctg tcccgccggc ttgcggcgat ggtggcagtg
     4741 taccaacttg aaattgttct gtcagttctc ggcaacgtgg cgccagcccc tacggcgtgc
     4801 cccgttctgt caggatgtgt gtgaaaacgg tagggcatgg tcggagctac cgaaagtggc
     4861 ggagacctgc cggtcatggc ggcagaattg gcaggtctcg gtcgccatgg cgtcctcggc
     4921 actgtcccac agactggctc ggtcggtttt cgtacgcatt ctcggaggtt gcccatctta
     4981 gacagggtgc cgcagtctcc ccggtgacat cctggccggc atgtgccgat ccgagcgacg
     5041 acggtataat ccgtggccta tgaaacctca acagacagac taccgtctcg gggccgtttc
     5101 cttcctcaac accaagccgt tgatcgaggg gctcggcgat gagtcgggga tacggctgga
     5161 gcttgcggtc cccgcagagc tggccgggat gctgcaggcc ggccgtgtcg acgccgcact
     5221 cgtgccggtc atcgatattg gccggtcggc cgggcgatgg aaacgcatct cggatgcggg
     5281 cattggcagc aacggcgata cccttaccgt ccaggtgttc tcgcgggtgc cgccggagga
     5341 catgaccacc ctccatgccg atacgcattc ccacacctcg gtgatgctgg cgcggctggt
     5401 gtggaaaggg tttcaccgaa gaccattggc cgtggttccg ctcaagcccg gaacca
//
LOCUS       DAOTPW010000318         5429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12666_length_5429_cov_5.163193, whole genome shotgun sequence.
ACCESSION   DAOTPW010000318 DAOTPW010000000
VERSION     DAOTPW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12666_length_5429_cov_5.163193"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="P5316_19905"
     CDS             complement(<1..134)
                     /locus_tag="P5316_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRR87281.1"
                     /translation="MHAREIGNGIHCIAMVHWERRLFDSLIPLPDGTSYNAYLVRDNS
                     A"
     gene            533..1741
                     /locus_tag="P5316_19910"
     CDS             533..1741
                     /locus_tag="P5316_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87282.1"
                     /translation="MRVSTATRLLIIGSTLSGAVFGAEVKRVAPEDFAILPWGGTKPD
                     PAVLKEIADCGFNLAGFVPPEGLDAVQAAGLKCIVSGHNTHVGDAEAKLDEDEIARRV
                     EPLVKQVGKHPALFGYYLRDEPTAHAFPGLANWVAVYRKVDPDARCYINLFPNVASIP
                     QLGVNTYDEYVESFVTIVKPGFISYDHYALMDDGSLKDGYFQNLESVRRIAQKYNLPF
                     WNIVLSNAHFHYAEPSENGLRFQAYTTLAYGARGISYFTYFAPPTGNYRLAPIDQFGH
                     KTATWDMLRNVNLQIHQLAPTYIKLKSVNVFHHPDVPKGCQDLTSSKFIQEVSGGRFV
                     VGEFEGPGGRSFVMVVNKDLHKSTSFTIRFKQAGRIMQVNSYSGGLHPWAGENNWLAP
                     GQGMLLSLEQ"
     gene            1889..2608
                     /locus_tag="P5316_19915"
     CDS             1889..2608
                     /locus_tag="P5316_19915"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR87283.1"
                     /translation="MRYSTKGLAAICAFAAAGCVDKVPVANVPPGVRPSVPDPAASRP
                     SVLLEAAPGEWVSLFDGKTLSGWRVLTEKAFVGHKEVRVDNGTIVMERGQLQTGIGWD
                     GAFPSENYEVWLEAMRTDGHDFFCGMTFPVGQEPCTLIIGGWGGSVVGLSNVDHMPAA
                     ENMTTNSMSFESNRWYVIHLRVTTKKIDVWIDDQHMISLEREGRSFSVWWEQEAARPF
                     GIANWDTGSALRHIRFRRLAN"
     gene            3136..4119
                     /locus_tag="P5316_19920"
     CDS             3136..4119
                     /locus_tag="P5316_19920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR87284.1"
                     /translation="MDETQIQTTVDARDAEILEMLLEVDEQASREDSRWAEKRAHVRK
                     TARIPCEVRFIGPDGESVLYTVGRTREVAAGGLSFLSRQHFARRCGILVSLSLSGGKI
                     RSLSAKVVYSRWLREGWYLTGVQFGAAEDPRLDPRNYNQVVVQEARYGDEKDSVEDDD
                     KVPSRQRVLQFLAMVSAAHRRTKSMVAKVVMASMSSDHVVRRACIPVLQSMAGREGLT
                     CLVSMLRDSNPEIQGEAAEALGMMGHPEALEPLQQLLHHQDDAVAVRAAEALARLGSW
                     SCKHVILRLLILEGPVSRRAARALGLLVGRDFRPNAEGVEQARKYAQEHDL"
     gene            4435..>5429
                     /locus_tag="P5316_19925"
     CDS             4435..>5429
                     /locus_tag="P5316_19925"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87285.1"
                     /translation="MMKVGLMGLGRGGQIVAEALLSSSWCQLAAVASRKSHRIDRFVE
                     RHPEITAYDDFRSLIVENPLDALFVAVPPFLRGKYLTLAAEHRCPVWMLSPAARRFDE
                     SVEIMERFEKARCPIVVSRSWGMEPSLQPDAVGLDRLGRFFLAVGGVYLCTGEDLDWR
                     GDSVRAGGGVLLDRGYELLDTVVKVMGLPATVYAAAKGVSRPGTRFPYDTEDTAALIC
                     QFSGGAVAAVTACWTSGPERCEIEFFGTGGTLRIDSRCVLCRDRAGERTLAELPRAAN
                     PLLASVEDFLSSLKSKSEHFRSSIRDHLATMAVIQAAYLSARTGQPESPGAILRM"
BASE COUNT          951 a         1497 c         1831 g         1150 t
ORIGIN      
        1 gcgctgttgt cccgtaccag ataagcgttg tagctggtgc cgtcaggcag gggaatcaat
       61 gagtcgaaca gccgccgctc ccagtgaacc atcgcgatgc aatggatgcc gttgccgatt
      121 tctcgcgcgt gcatgttttc tgtccctttt cggttcgctt gcgtctccaa tatggcctgc
      181 ctgttgtcga ctcatgccgc ccgcggccgg gccgtacgag aaacgccggg ggcaggaggc
      241 gatcgcggca agcccgtttt tcggctgttc gtcggcctac ttatgataga ccccatttgg
      301 gccgttggtc acaatggttt tcatgctgcc ggccgttggg cagcgttagc ccgcgccccc
      361 accggttttc gaccgaaggg gagtatactg aaatccgtgt ctttcggaca ggaaggcata
      421 ccagaagagc ggccggcggg ggggcgcggt tcgcgaggtt gccgattccc gggtatctat
      481 tagcataggg tgcggaagtc catctgcgct tgttcaacaa ggagacacga ccatgcgcgt
      541 atcaactgcg acacggctgc tcattattgg gtcaaccctg tcaggagctg tcttcggggc
      601 ggaggtcaag cgggtggcac cggaggactt cgccatcctg ccctggggcg gcacgaagcc
      661 cgacccggcg gtgctgaaag agattgccga ctgcggattc aacttggccg ggttcgtgcc
      721 ccccgagggg ctcgatgcgg tccaggcggc ggggctcaag tgcattgtct ccgggcacaa
      781 cacgcacgtg ggcgatgccg aggccaaact ggatgaagac gagattgcca gacgcgtcga
      841 accgctggtc aagcaggtgg gcaagcatcc ggccctgttc gggtattacc tgcgcgatga
      901 gccgacggcc catgcgtttc ccggtttggc caattgggtg gccgtttatc gcaaggtcga
      961 tccggacgct cgttgttaca tcaacctttt tccaaacgtt gcctcgatcc cccagttggg
     1021 ggtcaatacc tacgacgagt acgtggaatc gttcgtcacc atcgtcaagc ccggttttat
     1081 cagctatgac cactacgcgc tgatggacga cggttcgttg aaggacggct attttcagaa
     1141 ccttgagtcg gtgcgaagga tcgcccagaa gtacaacctg ccgttctgga acatcgttct
     1201 ctcgaacgcg cacttccact acgccgagcc gagcgaaaac ggcctgcggt ttcaggctta
     1261 cacgacgctt gcctacggcg cccgaggcat cagctatttc acctacttcg ccccgcccac
     1321 gggcaactac cgtcttgcgc cgatcgatca gtttgggcat aagacggcga cgtgggacat
     1381 gctgcggaac gtcaatctgc agattcatca gcttgcccca acgtacatca agctgaagag
     1441 cgtcaatgtc tttcatcatc ccgacgtgcc gaaggggtgc caggacctca caagcagcaa
     1501 gttcattcag gaagtgagcg gcgggagatt cgtggtgggc gagtttgagg gcccgggtgg
     1561 ccggtcgttc gtgatggtgg tcaacaagga ccttcacaaa tcgaccagtt tcacgatcag
     1621 gttcaagcag gccggtcgga tcatgcaggt caattcctac tccggcgggc tgcacccctg
     1681 ggccggtgaa aacaactggc tggcacccgg tcagggcatg ttgttgagcc tggagcaata
     1741 gaaccttacg gccggaaacc tgtgtgggtt gcgcgcgtgt ttccggatcg cagtgcgctt
     1801 ggattgcggc ccgcaaaatg gcggttcgcc tcagggtctg aggttgatgc atcgcggcgt
     1861 tggttcatcg tgagcggaga actggtcgtt gagatactcc acgaaaggtt tggcggcgat
     1921 ttgtgcgttt gcagcggcgg gttgtgtgga caaggtgccc gtggcgaacg ttccccccgg
     1981 tgtccgaccg tcggtcccgg atcccgcggc gtcccggccg tcggtgctgc tggaggccgc
     2041 gccgggtgaa tgggtcagcc ttttcgacgg caagacgctg agtgggtggc gtgttctgac
     2101 cgagaaggcg tttgtgggcc acaaggaagt tcgcgtggat aacggtacga tcgtgatgga
     2161 acgcggccag ttgcagacgg gtatcggctg ggatggtgcc tttccgagcg agaactacga
     2221 ggtttggttg gaggccatgc gaaccgacgg gcacgatttc ttctgcggga tgacttttcc
     2281 ggtcggtcag gagccgtgca cgctgattat cgggggctgg ggtggttcgg tggtagggtt
     2341 gtcgaacgtc gaccacatgc ccgcggccga gaacatgacc accaacagca tgtcgtttga
     2401 gagcaaccga tggtatgtca tccacttgcg ggtcacgacg aagaagatcg acgtttggat
     2461 cgacgatcag cacatgatca gcctggagcg ggaaggtcgc agcttcagcg tttggtggga
     2521 gcaagaggcg gcccggccct tcgggattgc caattgggat actggaagcg ccctccggca
     2581 catacgcttc cggcggctgg caaattgaaa gcccgtcacc cagattgtgc ggccttttcg
     2641 gtgtcatccg tgccgtgtta tcggacgcgc ggacaagcca tacgggtgtc ctgggggtca
     2701 gctttcgaag atgtggtaaa taccccccat gttctggccg aatcttgaag taccgtcagc
     2761 gttgctttcg ccgttgtcgt cctgtctgag ggcgactggc aggcggtggt ctccggttac
     2821 cgatgagcag gccgagccgc tgggttgatg gcaagcgtcg atttccgccg gggggcgcga
     2881 cccacgctct tattctttga gggccccccg cagccagggg ttgaaggact gaaaaagacg
     2941 cggaggcggg ttatcaggaa gttgaccgtt cattcaggcg tcttcggcgg ccggccgatt
     3001 tgccttgaga tcgcttttca ggcgtggggc tgacttgcgg cccttgagcg aggtcgtggg
     3061 ctcaacgaag cgcgtaagtt gttccgatca tagcaaaggt tcaggactgc aagcgagatc
     3121 ctattggccc gggccatgga tgagactcag atacagacga ccgtggatgc gcgggatgcc
     3181 gagatcctgg agatgctcct ggaggtggat gaacaggcga gccgcgagga ctcccgctgg
     3241 gctgagaaga gggcccatgt gcgcaagacg gcgcgcatcc cgtgcgaagt caggtttatc
     3301 ggccccgacg gcgagtcggt gctctacacc gtgggaagaa cgcgtgaggt tgccgcgggt
     3361 ggcttgagct tcttgagccg tcagcatttt gcccggcgtt gcggtattct ggtcagcctt
     3421 tcgctcagcg gcggcaagat ccgttctctt tcagcaaaag tggtctattc gcgctggctg
     3481 cgggaaggat ggtacctgac cggcgttcag tttggtgctg cagaagaccc gcggcttgat
     3541 cctcggaact acaaccaggt cgttgtccag gaagctcgct acggcgatga aaaggactcg
     3601 gtcgaggacg acgacaaggt cccgtcgcgt caacgagtgc tgcagtttct ggcgatggtt
     3661 tcggccgctc accggcgaac gaagagtatg gtcgccaagg tcgtcatggc ctcgatgtct
     3721 tcggatcacg ttgttcgtcg cgcgtgcatt cccgttttgc agagcatggc cggcagggaa
     3781 ggtttgactt gtcttgtctc catgctgcgg gatagtaacc cagagattca gggcgaggcg
     3841 gccgaggcgc tggggatgat ggggcatccg gaggccctgg aaccgctgca acagcttctg
     3901 catcatcagg acgacgccgt tgccgtccgc gccgccgaag ccctggcccg gttgggtagc
     3961 tggagctgca agcacgtgat cttgcgcctg ctgattctgg aagggccggt cagtcgtcgc
     4021 gcagcccggg cgctgggcct gttggtggga cgcgatttcc gtcccaacgc ggaaggcgtt
     4081 gagcaggcac gcaagtatgc ccaggagcac gatctctgat cgcctcgcct cgttcatagg
     4141 cggatcaagg gtttccttac cttggaacct tacggccaga catctgtgcg gcttgcgcgc
     4201 atgtctctgg atcgcagatc gcttgggtta cggcccgcga aaaggcggtc cgcctcaggt
     4261 gttcatttct gccacccgct tgctgtttga gagaggttcg agtatctgtc tcacatccat
     4321 cgtgtgatag cggtctcgcg tccggcgggt gacggcccgg cgtgagcttc tcggcacgac
     4381 tgtaccgctg caacgagcgc tgtcggcgca ccgatggtcg gcaaggaggc accgatgatg
     4441 aaggttggcc tgatgggcct tgggaggggc gggcagatcg tggccgaggc gcttttgtca
     4501 agctcctggt gtcaactggc cgcggtggca agcaggaaat cgcatcggat tgaccggttc
     4561 gtcgaaaggc acccggagat cacggcctac gacgacttcc ggtcgctcat tgtcgagaat
     4621 ccgctggatg cgcttttcgt ggcagttcct ccctttcttc gcgggaagta tctgacgctg
     4681 gcggctgagc atcgctgccc ggtctggatg ctttcaccgg cggcgcggcg gttcgacgag
     4741 tcggtcgaga tcatggaacg gttcgaaaag gcgaggtgtc cgatcgtggt gtcgcggagt
     4801 tgggggatgg agccgtcgct gcagccggac gcggtcggcc tggatcggct gggacgcttc
     4861 ttcctcgcgg tgggcggggt gtatctctgc actggtgagg accttgactg gcggggagac
     4921 tccgtccggg ctggcggcgg tgtgctgctg gaccgcggtt acgagctgtt ggacaccgtg
     4981 gtgaaagtaa tggggttacc agcgacggtg tatgcggccg ccaagggcgt atcgcggcct
     5041 ggaacccggt ttccctatga caccgaggat acggcggcgc tgatctgtca gttctccggc
     5101 ggagcggtgg cggcggtgac cgcttgctgg acctccggac ccgagcggtg tgaaatcgag
     5161 tttttcggga ccggcgggac gcttcgtatc gattcgcggt gcgtgctgtg tcgcgaccgg
     5221 gctggggaga gaacgctggc agagctgccg cgggccgcca atccgttgct cgcctcggtc
     5281 gaagattttt tgtcctctct caagagcaag tcggagcact tccggtcttc gattcgcgac
     5341 cacctggcga ccatggccgt gatccaggcg gcgtacttgt ccgcgaggac gggccagccc
     5401 gaaagcccgg gtgccattct cagaatgca
//
LOCUS       DAOTPW010000319         5428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12672_length_5428_cov_6.320491, whole genome shotgun sequence.
ACCESSION   DAOTPW010000319 DAOTPW010000000
VERSION     DAOTPW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12672_length_5428_cov_6.320491"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..923)
                     /locus_tag="P5316_19930"
     CDS             complement(138..923)
                     /locus_tag="P5316_19930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HRR87286.1"
                     /translation="MARKQGVRSYDFSTYPSPDSVLGQQYYYVTLRFWPSLVFVTPFL
                     VVYEIGRLVNQAGPTSPNEQLVAAWLIESLVRTIGVSGYFPAPTLLTVAVLLACHLVA
                     KHPWKFDIWVLAGMLGESLIYTIPLIVLRRIPHAAVLATGSASYDTWLNEVIRSFGGG
                     VYEELVFRFICMNVLHIFLVDLCRLPRTPASVAIIFASAVVFAASHHWPLGSEQFNQT
                     EFLFRTFAGLYLAGLYFFRGFGVAAGCHSFYNVLVYTLVALRG"
     gene            1552..3537
                     /locus_tag="P5316_19935"
     CDS             1552..3537
                     /locus_tag="P5316_19935"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRR87287.1"
                     /translation="MRRTDKDSFMPSGLYEQLKNENALPSPTGVVLKLLRLAGDEDST
                     VEAIAATIESDPALAARVLKLVNSPIAGTSRRIGSVSRAVVLLGLRTLKHLALSISLI
                     SNHRTGRCENFDFTRFWRESVARAVSAQHLFARLGGLPADEAFTLGLLGKIGQLALAV
                     GCPEQYARALARARADDIPGLLEIEREIFGTDHNDLSADMMSGWHVPDALCEIVRKQD
                     AADWRGTNAVAPADVTRATLRLASLIAAALNRREADPDLAAGLEHQAGLLGLTAAELS
                     RLMDAVREEWLETTAMFLLKPDQTSEIEQARVGSYGRAYRQSDVVSPTASEKNARPAG
                     GDSLRILVVEDDPDQLALLTKHLGEAGYRVMTASNGAEAICVDLAQAPQMIITDCQMP
                     GIDGLELCRRLRSQEGAELAYIIMLTGYLDRTQMVEALNAGADDVLSKPYGREELLAR
                     VRAGQRVVRLKASLAARALEVSAYNVKLSAYNDRLQVMASQDELTGLFNRREAMLSLA
                     EHWALSVRQNSPLSCVMVDIDRFKRINDRYGHRTGDLVLVETANRLRSTVRAGEKLCR
                     FGGEEFLIVCPGSSAEASRRLAERLRKAVASSPIKARGDKCNVTISLGVAERDEEMSS
                     SDDLLAAADAALYAAKRAGRNRVHVAGPRERDPAGKG"
BASE COUNT         1004 a         1536 c         1842 g         1046 t
ORIGIN      
        1 catgcggcag gtctcggtcg ctgtggcgac ctcgacgctg ccctaccgtg accgccggcc
       61 ggccgggaat tccgtcccca ttttggcgga tcgtgaggat aggaatgctc cacgcagcag
      121 gccaaatgtc gccggtctca ccctcgcagg gcgacgagcg tatagaccag cacgttgtag
      181 aaggaatgac acccggcggc gacgccgaag ccgcggaaga aatagagacc tgccaggtac
      241 agccctgcga aggtgcggaa gaggaactcg gtctggttga actgctcgct tcccaacggc
      301 caatgatgcg aggcggcgaa gacgaccgcc gaggcaaaga tgatcgccac actggccggg
      361 gttcgcggca gccgacacag atcgaccaga aagatgtgca gcacgttcat gcagatgaac
      421 cggaacacca gttcttcgta gaccccgccg ccgaagctcc tgatcacttc gttgagccag
      481 gtgtcgtacg aagccgatcc ggtcgcgagg accgccgcat gcggaatgcg ccgcaggaca
      541 atcagcggaa tggtatagat cagactctcg cccaacatgc cggccaggac ccagatgtcg
      601 aacttccacg ggtgtttggc cacgaggtgg caggccaaca acaccgccac ggtcagcagg
      661 gttggggcgg ggaagtagcc gctgacgccg atggttcgaa cgagcgactc gatcagccac
      721 gcggccacca gttgctcatt gggactggtt ggtccggcct gattgacgag ccggcctatc
      781 tcgtaaacca cgaggaacgg cgtgacgaag acgagactgg gccagaaacg aagggtgacg
      841 tagtagtact gctgtcccag caccgaatcc ggcgacgggt aggtactgaa gtcgtagctt
      901 ctgacgcctt gtttcctggc catgcgggcg attatcgtcc cagccgtttc gttggcaatg
      961 gttagcggtg tcggcccggc aacgaggttg ttgcggcggc ggcggtccga ttcacgaaat
     1021 cctccggcgt tgctctctcg aaggcggatt gacgccatgc cggcgggcgg tccgcggcag
     1081 cgtgacccgt acttgcggtt tgttaagaac cgcagggggt tgtcaggacg gatgcagagg
     1141 tggaaaacag ggccgcggct tcgagatgag gatcagaggt cgatcagtca cacttggaaa
     1201 tcgccagcag ataaggacaa gagccagatc gttcggatgg cctgcttaag gtcgagtcga
     1261 tgcatcaaac agggaagtgc ggcgcccaag gtgacggaaa gggcatgcca ggccgttggc
     1321 cgcacggagc gggcatctca gtcattgcac gtctttttca caaacacctt acactgcgtt
     1381 tctgcttgct catgaggttc gcacgcaatc ttcgcttgag agtcaactga tcgtgaattc
     1441 ccatgtggcg atgtccgatt attagtggcg agccgacgcg ccggttgaat tgcgcgtgtc
     1501 tcggtcgacg ggagcgggct gggaaggcgt ccccatcttc cgagcagcaa gatgaggcga
     1561 actgacaaag attctttcat gcccagtggt ctttacgaac agttgaaaaa cgagaacgct
     1621 ttaccctctc ccacgggggt ggtgctcaag ctcctgcgac tcgccggcga cgaggattcc
     1681 acggtcgaag ccatcgcggc caccatcgag tccgatcctg ccttggcggc acgcgtgctg
     1741 aagctggtca attccccgat tgccggtacc tcgcgacgga tcggctcggt atcccgcgcg
     1801 gtggtgctgc tcggcctgcg gacgctcaag cacctcgcgt taagtatctc gctcatttcg
     1861 aatcaccgga ccgggcgctg cgagaacttc gatttcacac gtttttggag ggaatctgtt
     1921 gcaagagccg tatcggcaca gcatctgttt gcccggctgg gaggcctgcc ggccgacgag
     1981 gcattcaccc tgggtctgct cggcaagatc ggccagttgg cgttggcggt gggatgcccg
     2041 gagcagtatg cccgggctct ggctcgggcc cgggccgatg atatacccgg tttgctcgag
     2101 atcgaacggg agatttttgg aacggaccac aacgatctgt cggcggacat gatgagcggc
     2161 tggcatgtgc cggacgctct ctgcgagatc gttcgcaagc aggacgccgc ggattggcgg
     2221 ggtacgaacg ccgttgcccc ggccgacgtt acccgcgcca cattgcgtct tgcctccctg
     2281 attgcggccg cattaaaccg gcgggaggcg gacccggatc ttgcagccgg gctcgaacac
     2341 caggccggcc tgcttgggtt gacggcggcg gagctcagcc gactgatgga cgccgtccgc
     2401 gaagagtggc ttgagacgac agcgatgttt ttgttgaagc ccgaccagac atcggagatc
     2461 gagcaggccc gagtcggctc ttacgggcgg gcgtatcgtc aaagcgacgt cgtatctccg
     2521 accgcgtcgg aaaagaatgc acgaccagcg ggcggtgatt cgctgcggat tctggttgtt
     2581 gaagacgatc ctgaccagtt ggccctcttg accaagcacc tcggcgaggc aggctaccgg
     2641 gtgatgactg cttcaaacgg cgccgaggcg atatgcgtgg atcttgctca agcgcctcag
     2701 atgatcatca ccgactgcca gatgccgggg atcgacggac tggaactatg ccgcaggttg
     2761 cgatcgcagg aaggcgcgga actggcgtac atcatcatgc tgaccggcta cctggacagg
     2821 acgcagatgg tggaagccct taacgccggg gcggacgacg ttctctccaa gccgtacggt
     2881 cgcgaggaac tcctggcccg ggtacgtgcg ggccagcggg tggtgcggct caaggccagc
     2941 ctggcggcgc gtgcccttga ggtctccgcc tacaacgtca agctctcggc ttacaacgac
     3001 cggctgcaag tcatggccag ccaggacgag cttaccggat tgttcaaccg gcgggaggcg
     3061 atgctttccc tggcggagca ctgggcgtta tcggtccgcc agaacagtcc gctctcgtgc
     3121 gtcatggtcg acattgatcg gttcaagcgt atcaacgaca ggtacggcca caggacgggc
     3181 gacctggtgc tggtcgaaac ggccaatcgc cttcggtcga cggtgagggc tggcgagaaa
     3241 ctgtgtcggt tcggcggcga ggagttcctg atcgtctgtc cgggctcatc cgccgaggca
     3301 tcgcggcggc tcgccgagcg gcttcggaag gcggttgcgt cgagccccat caaggccagg
     3361 ggagacaagt gcaacgttac gatcagcctg ggggttgccg aacgggacga ggagatgagc
     3421 tcgtcagacg atttgctcgc cgcagccgat gcggctttgt acgccgccaa gcgagcgggc
     3481 agaaaccgcg tgcatgtggc gggcccgcgc gagcgagacc cggctggcaa ggggtgagac
     3541 ggctgccagg cctgacgctg cggcttgcac gaacaaccgg cttggcacga tccgtgcaac
     3601 gcgcatcggg atgatccatc gccggccgaa gcctgcctgg cgagtcgttc cgacctctgc
     3661 ggccctgggc ttcagtaatt cctcccgcat acggctccgc ggcaacggca cggtcggcgt
     3721 agcacgtaag tctttttttc ataatggttt acaatactat tgggttcgtt tggcgccagc
     3781 ggtttttcgg cgtccagggt tcagcaatca ggaagacggg tgtcacaacg ggacgtggcc
     3841 gccggattct ttacccattt tctccagatt gcgcaggctt gggttggttt ggcgccgggg
     3901 aggtgtggac catcaggtct cgggtttcag ccagacacgc ccggggcagg cctgcgcttg
     3961 gccgagtgcc gaacgcagac cgctgatcgc ttctggcggg tgggttcctt tggagaaacg
     4021 acttgtcaaa gaacgttggc ctgcgggcag ccggagggcg tgtacggtcc cagccggttg
     4081 aggacggtat gccggtggag tatctccgcc ggtcccgttg ggaggataac aggcgggggt
     4141 gctcgggcaa gaaggatgca agcggaacgc ggaggggaga agcttttagc tcttagctgt
     4201 tggctcttag cccagccgga cgtgggggcc agagagtgcc ggacggggag gggaagccga
     4261 agcgcggaat gggcggaaga tacaacaggc aagacggggt atcggttctc agtggtcggt
     4321 gatctgcccc aacgggttct ggacattatg gcgatgcgca gggataggca gtgtcgaccg
     4381 ctgatcgctg agtgccggcg ccttttttgt gcccgcagat cacgcggatt gacgcggata
     4441 aggaacgcgt ggggggcaga ctgctcttcg aagccgcggt ccgtcgcgcg gtatgagcaa
     4501 ttccccgatc atttcggttg agggtcgacc gccgacagct tcggtttagc ctcttccatc
     4561 gggactggtg cgcattttgc gcgcatgttt ccggatcgcg gtacgcttgg gttgcggccc
     4621 gcaaaaaggc ggtccgcctt gggattggat atgtgggcat gtggcccatg ctaccgggca
     4681 aacaccccat ggtccagctt gacgttgcag gcatgaacag gttcgttgat cgcagccgcg
     4741 cgttcttctg atcgccctgc ctggaaagct tcaggcaacc acaagaaggg ttcaggcgcc
     4801 atcgtgagac acgagaccgg acgggttcgt ttttgggggc gactgtcttg agccactatt
     4861 ccgccgtctt catccttgcc ggccttcgcg gccgcccctg cggacacaaa tgttctccgg
     4921 ttccgggcgt ccgcccgtct gagccatcga ggccgacccg ccgaaaggcc tcccggagat
     4981 caggcactga ggcagcgcat ctggttccat tccggtcacc gtctggttca tgcttgacat
     5041 ttcagtactc gccacgcgaa tcagacgcga gcaccagctc tcctgggatg tcaagggttc
     5101 atcgttcgga cccgatcaca tgggtgcgtg acagtgtggg cgtgtatgcc gaccggtcga
     5161 ggttgggcga ggagcctcct tcagggggtg ttcccgccaa gggcgggctg atagaccggg
     5221 cgatatacgg gcggttggtg gtcggccggg gttgtagccc gttttagagg gcttaccgag
     5281 gtatcttggg ttcgcccgtg gtgagggggg aaggtgtcgg tgttgtggtc ggggctgccg
     5341 gatcaggggg tagcgatcct gctcgacgtt ttgtgtgccg acgagggcgt cggcactact
     5401 tggggcgtcg gcgctacttg gggtgtcg
//
LOCUS       DAOTPW010000320         5391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12769_length_5391_cov_6.069715, whole genome shotgun sequence.
ACCESSION   DAOTPW010000320 DAOTPW010000000
VERSION     DAOTPW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12769_length_5391_cov_6.069715"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            666..1850
                     /locus_tag="P5316_19940"
     CDS             666..1850
                     /locus_tag="P5316_19940"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRR87288.1"
                     /translation="MIRSFNRWVWIGGLAVFVLVGACSQQGGPKGPGDMTEGKCEMKV
                     EKENWGTTLDGARVDLYRLTNAHGMQADIATYGGIVVRLMVPDKNGQFDDVVLGFDSL
                     AGYLKGHPFFGALVGRYGNRIAKGRFTLDGVEYNLATNNMGNHLHGGLKGFDKAVWAA
                     EPVQRSEAVGLRLSHTSKDGEEGYPGNLECTVTYLLTNKNELKIEYEATTDKATPVNL
                     TNHTYFNLAGAGSGDVLNHLMMLNADRFLPVDKTLIPTGELRPVKGTPFDFTKPTPIG
                     KNINADDEQVKFGNGWDHCFVLNKSQPGELTLCARVTEPTSGRVMEMHTTEPGVQFYS
                     GNFLDGSNVGKGGKAYKFRYGFCLEAEHYPDSPNKPEFPSTILKPGEIYRQTTIYRFS
                     TK"
     gene            1910..3994
                     /locus_tag="P5316_19945"
     CDS             1910..3994
                     /locus_tag="P5316_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275090.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRR87289.1"
                     /translation="MGLADILVFVLFVAAVIAVGLWKSRGEETRSEHGAQDYFLAGRG
                     LTWWLVGFSLIAANISTEQFVGMSGKAADWLGMAIASYEWMAAITLVVVAFLFLPKFL
                     KSGIYTIPEFLEYRYNTFARTVMAISTLIILVGVPTASVIYSGAKVITVNFQGQSVFG
                     FDLGSIEVGCWIIGTLAAIYVFAGGLKACAWADLIQGSALIVGGVIIAFLAMRWLGNA
                     DPGALAATATAPVDATALGGAGALERLRMLNAGDWATGKLHMVKPASDPEVPWTALVV
                     GLWIPNFFYWGLNQYITQRTLGSKSLAEGQRGVVFAAFLKLLVPFVVVIPGILAYNLF
                     SGDLRNAAVVKNAKAIAAFAPDLYPQLSDDVKPDAADPLYVNDLKAINRQLDRARTKP
                     PQDLTVFRFTGSFAEDHTADAACIVSFNAAALGVELPNEWLAQAKSDPQLLMNRNADL
                     LKKAGDKEFKGTVRELVLRDYDAAFPVLIKNLLPLGSGLKGFVLAAIFGAVVSSLASM
                     LNSSSTIFAMDIFRKLKKDATQFQLVTTGRICVVAFVLIAMLIAPRLDNPKFGGIFTF
                     IQEFQGFISPGILAIFLFGVLVHRAPRACGTAGLLLNPVLYGGFKWWIGPWMCAHGLW
                     WSKEMSFLDRMAVCFFIVLAVLTVMRLAAPMPRPVDLPVNPKMNLESKGDVKIWGTLV
                     VLATLALYVVFW"
     gene            4469..>5391
                     /gene="thrS"
                     /locus_tag="P5316_19950"
     CDS             4469..>5391
                     /gene="thrS"
                     /locus_tag="P5316_19950"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRR87290.1"
                     /translation="MPQITLPDGKVLDVPAGKTVADVAAMINPGLARAAIAAKLDGAI
                     VDLSTPVTRDGRLSILKADADDPDSLYVMRHSCAHVMAEAICSLFPQAKLVYGPPVEN
                     GFYYDIDLDRPLSQEDFAAIEAKMAEIVKADRPFTRYEMSREEGMAKLRQEGSRYKID
                     NAERAEGPLSFYVTGTERGSDFEDLCRGPHVPSTGRIGAFKIMQVSGAYYRGDQKEKQ
                     LQRVYGTAWPTAKDLKRYLDQLEEARKRDHRKIGQELGLFLIDPLVGSGLVLWKPKGA
                     IVRYQLESFLRGELTKLGYQAVYTPQIGKLGL"
BASE COUNT         1028 a         1570 c         1720 g         1073 t
ORIGIN      
        1 ctgagcggag atgggccgac gggggcgtcg gccctacccc ccgcatgccc acgcaaagcc
       61 gtgggcatgg caccccgcgg acctgcccta cctgctcggt ggaggattga catcgtcatg
      121 aatgccgaat gcggaaagga ttccgcagcg tgtacggccc ggaaaggatt ccgggccgag
      181 cgggcgagaa tgcggcccgg aaaggattcc gggccgagcg gatgggacgt gactcgggcc
      241 ggatgatcga catcgggatt tgaggaattc ggggtcttca gcagaatctg tgggtgggat
      301 tggggctccg tcccaaggcc tgggatcttt tgaggcatcg ctccggtgtt ccagaggcgg
      361 caaacacggg cgtttttgtg ggggaggacc atgttgcacc cccgaccaac accgcccaat
      421 gcgggtgcgg aaagcggact cggaaatccc gtctagacag caaggataaa aaagcacatg
      481 gatacgggtg tttcagcgcg tcaacgagtc aaaacgaccc acagattctg ctgaggagat
      541 caattcaatt ctcaactcgc aactctccat tctccattcc cttctcttct tgcaagccat
      601 cggctgtgcg tttaaccttt gaacgatcga caggaaatca ggtcacaggt cgaaagggca
      661 cagacatgat tcgatcgttc aatcgttggg tatggatcgg cggtttggcg gtgttcgttc
      721 tggtcggcgc gtgctcgcag cagggcggac ccaaggggcc aggtgacatg acagagggca
      781 agtgcgaaat gaaagtagag aaggaaaact ggggcacgac gctcgatggg gccagggtgg
      841 acctttatcg gctgaccaac gcccatggaa tgcaggcgga tatcgccact tatggcggca
      901 ttgtggtccg gctgatggtt ccggacaaga acggccagtt cgatgacgtc gtgctggggt
      961 ttgactcgct ggcgggttac ctgaaggggc acccgttttt cggggccctg gtcggccgtt
     1021 acggcaaccg catcgccaaa ggccggttta ccctcgacgg cgtcgaatac aacctggcga
     1081 ccaacaacat gggcaaccat ctgcacggcg gcctgaaagg cttcgacaag gccgtctggg
     1141 cagccgagcc ggtgcagcgc agcgaggcgg ttggcctcag actctcccat acgagcaagg
     1201 atggggaaga ggggtacccc ggcaatctcg agtgcacggt gacgtacctt ctgaccaaca
     1261 aaaacgagct gaagatcgag tatgaggcga cgacggacaa ggcgacgccg gtgaacctta
     1321 ccaaccacac gtacttcaac cttgccggag ccggcagcgg ggacgtcctc aaccacttga
     1381 tgatgcttaa tgccgaccgc ttcctgccgg tggacaagac gcttattccc acgggcgagt
     1441 tgcgcccggt caagggcacg ccgttcgatt tcaccaagcc cacgcccatc ggcaagaaca
     1501 tcaacgccga cgacgagcag gtgaaattcg gcaacggctg ggaccattgc ttcgtgctga
     1561 acaagtccca acccggcgaa ctgacgctgt gtgcccgcgt gaccgagccg accagcggcc
     1621 gggtgatgga gatgcacacc accgagccgg gtgtgcagtt ctactccggc aactttctcg
     1681 acgggtccaa cgtcggcaag gggggcaagg cgtacaagtt tcgctatggt ttctgcctcg
     1741 aagcggagca ttaccccgat tcgccgaaca agccggaatt cccgtccaca atcctcaagc
     1801 cgggtgaaat ctaccggcag acgacgatct accggttcag cacgaagtag atgcgacgag
     1861 cttgcttgcg gcaccggtct ccccgccggt ggccggatag gagcgtatca tgggacttgc
     1921 cgacatcctc gttttcgtcc tgttcgtggc cgcggtcatc gccgtcggcc tttggaagag
     1981 ccgcggtgag gaaacgcgca gcgagcacgg tgcgcaggac tacttcctgg ccggccgggg
     2041 gttgacctgg tggctggtcg gtttttcgct cattgccgcc aacatctcga ccgagcagtt
     2101 cgtcggcatg agcggcaagg ccgcggactg gctcggcatg gccattgcca gctatgagtg
     2161 gatggcggcc atcacacttg tcgtcgttgc gttcctgttc ctgcccaagt ttctcaagag
     2221 cggtatttac acgatcccgg agttcctgga gtaccgttac aacaccttcg cacgcacggt
     2281 gatggccatt tcgaccttga tcatcttggt cggcgtgcct acggcatcgg tgatctactc
     2341 gggcgccaag gtcatcacgg tgaatttcca gggccagtcc gtctttggct tcgacctggg
     2401 cagcatcgag gtcggctgct ggatcatcgg cactctggcg gcgatctatg tgtttgccgg
     2461 cggattgaag gcctgtgcct gggccgacct cattcagggc tcggcgctga tcgtcggcgg
     2521 cgtcatcata gcgttcctgg ccatgaggtg gctgggcaat gccgatccgg gcgctcttgc
     2581 cgccacggcc accgcgcccg tcgacgcgac cgccctgggc ggtgccggag cgttggaacg
     2641 tcttcgcatg ctgaatgccg gcgattgggc gaccggaaag ctgcacatgg tgaagccggc
     2701 gtcggacccc gaggtgccat ggaccgccct ggtcgtgggc ctgtggattc cgaatttctt
     2761 ctattgggga ttgaaccagt acatcaccca acgcacgctg ggctcgaagt ccctcgcgga
     2821 agggcagcgg ggtgtggtct tcgcggcgtt tctgaagctg ctggttccgt tcgtggtagt
     2881 gattccgggc atcctggcgt acaacctgtt ttccggcgac ctgcgaaacg cggccgtggt
     2941 caagaacgcc aaggcgattg ccgcgtttgc tcctgatctc tacccgcagc tttccgacga
     3001 cgtcaagccc gacgccgctg atccccttta cgtgaacgat ctcaaggcga tcaacaggca
     3061 actcgaccgc gcgcggacca agccgcccca agatctgacg gtctttcgtt ttacaggaag
     3121 tttcgccgag gatcacacgg cggatgcggc gtgcatcgtg agtttcaacg ccgcagcact
     3181 tggcgtggaa ttgccgaacg agtggctggc tcaggcaaag tctgacccgc agcttctgat
     3241 gaatcgcaac gccgatctcc tgaagaaagc cggcgacaag gaattcaagg gcaccgtccg
     3301 cgagctggtg ctgcgagatt acgacgcggc cttcccggtg ctgatcaaga acctcctgcc
     3361 cctgggaagc ggactgaaag gcttcgtgct cgcggccatc ttcggcgcgg tggtcagctc
     3421 gctggcctcg atgttgaact cgtcgtcgac catcttcgcg atggacatct tccgcaaact
     3481 gaagaaggac gccacgcagt tccagttggt caccacgggc cgcatctgcg tggtcgcgtt
     3541 cgtcctgatc gcgatgctca tcgcccccag actggacaat ccgaagttcg gcggcatttt
     3601 taccttcatc caggaattcc agggttttat ctcgccgggc attctggcga ttttcctgtt
     3661 cggtgtgttg gttcacaggg ccccccgggc ctgcggcacg gcaggtctgc tcctcaatcc
     3721 cgttctgtac ggcggcttca agtggtggat cggtccctgg atgtgcgccc atggcctgtg
     3781 gtggtcgaag gaaatgtcct tcctggaccg catggcggtg tgtttcttta tcgtgctggc
     3841 tgtgcttacg gtcatgcggc ttgccgcccc gatgccccgt ccggttgacc tgccggtgaa
     3901 ccccaagatg aacctggaga gcaagggcga cgtcaagatc tggggtaccc tggtcgttct
     3961 ggcgaccctg gcactctacg ttgtgttctg gtgagcatac agcagaccgg ggatcgtcgc
     4021 cacatcggtt gctgatgaac ggtattccgt ccggggcttt ggggtagcca tcgactcaat
     4081 gccgcaggca tgggaacggg gcaacaacgc taactcgttc tgtggactgg ttttgccgat
     4141 attggatacg ggcgattgtc gttttctgtt gacgcccctg tggggcgtgc tatagtgaga
     4201 tcggccgggg ctgagcgacg gcttgcgtgg gcggccttcc ggcaaggtga tgtccggcgt
     4261 tgcgggcatc ggatatagaa ctgacaacgg catgcgattc acgacgcgca cgacggtttg
     4321 atgtggcatt cgcggacgag ttcggcgaac tgcagatcgt gcgcgagtga gtgaatcgca
     4381 tgccgtttct cttttggcgg cgggcgggtt gccggccctg gtgtgaaacg ggcggccgcc
     4441 ggcccggctc ttgtggaagt ggtgaacaat gcctcagatc acactgcctg atgggaaagt
     4501 gctcgacgtg ccggcgggaa agaccgtcgc ggacgtcgca gccatgatca accccggcct
     4561 ggcccgcgcg gccatcgccg ccaaactcga cggcgctatc gtcgatctga gcacgccggt
     4621 cacacgggat ggccgtttgt ccatcctcaa agccgacgcc gacgaccccg acagcctgta
     4681 cgtcatgcgc cacagttgcg cccatgtcat ggccgaggct atctgctcgc tgttcccgca
     4741 ggccaagctg gtctacggcc cgccggtcga gaacggcttc tattacgaca ttgatctgga
     4801 ccggcccctg tctcaagagg acttcgcagc catcgaggcg aagatggctg agatcgtgaa
     4861 ggccgatcgg ccgttcaccc gttacgaaat gagccgcgaa gaaggtatgg cgaaactccg
     4921 tcaggagggc agccgataca agatcgacaa tgcggaacgg gcggaagggc ctctgagctt
     4981 ttacgtgacc ggcacagaaa ggggcagtga tttcgaggat ctgtgtcgcg gtccccacgt
     5041 acccagtacc ggccggatcg gggccttcaa gatcatgcag gtcagcgggg cttattaccg
     5101 cggcgaccag aaagagaagc agcttcagcg ggtttatggc acagcctggc cgaccgccaa
     5161 ggacttgaag cgttacctgg atcaactgga ggaggccagg aagcgcgacc accgcaagat
     5221 agggcaggaa ctcgggctgt ttctcattga tccgctcgtc ggcagcggct tggtgctctg
     5281 gaagcccaag ggtgcgattg tccgctacca gcttgagagc ttcctgcggg gcgagttgac
     5341 caagcttggc taccaggccg tgtacacccc ccagatcgga aagctgggcc t
//
LOCUS       DAOTPW010000321         5373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12813_length_5373_cov_5.999624, whole genome shotgun sequence.
ACCESSION   DAOTPW010000321 DAOTPW010000000
VERSION     DAOTPW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12813_length_5373_cov_5.999624"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..712)
                     /gene="ssb"
                     /locus_tag="P5316_19955"
     CDS             complement(272..712)
                     /gene="ssb"
                     /locus_tag="P5316_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRR87291.1"
                     /translation="MASFNRVILAGNLTRDPQLSYTPNNTAVCEFGLAVNRRWRDRDG
                     NQKDEVCFVDVTSYGRQAETINQYMKKGSGILIEGRLRFRQWTSKEGQSRNKLDVVVE
                     NFTFLGSGAGAAGRPADRPAASVVEEGPPPDDFGPPVENPDIPF"
     gene            complement(716..1168)
                     /gene="rpsF"
                     /locus_tag="P5316_19960"
     CDS             complement(716..1168)
                     /gene="rpsF"
                     /locus_tag="P5316_19960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRR87292.1"
                     /translation="MLRKYEGMFLFDPVVMSDWEAIQGEINRMFDRAGARVIACAKWD
                     ERRLAYEIRGRKRGIYVLTYFEADSSRIADIERDVQLSEAALRCLIVRVDHMTEDEMK
                     EAAAKPASHAAPDDDRSDRSGEGRRSRRRESEVTVPDVTEETPAETEE"
     gene            complement(1218..1784)
                     /gene="pth"
                     /locus_tag="P5316_19965"
     CDS             complement(1218..1784)
                     /gene="pth"
                     /locus_tag="P5316_19965"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRR87293.1"
                     /translation="MKLIVGLGNPGGKYEHTRHNVGFRTVDELSRRWQIEVTRGRFSG
                     LCGSGSIRDHKVLLLKPMTYMNLSGRSVREALTFHKLSRGDLLVVLDDMALPLGRLRL
                     RPGGSAGGHNGLISVIEQAGGEDFARLRIGIESVEGSRMVNHVLGEFTAVEQPRVEQA
                     IARAADAVECWLTEGIDSAMNRFNRPEE"
     gene            complement(1820..2476)
                     /locus_tag="P5316_19970"
     CDS             complement(1820..2476)
                     /locus_tag="P5316_19970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRR87294.1"
                     /translation="MEIAQLKAETRQGVGSAVARRLRKAGKLPAVLYGHGQRPENLVV
                     SAHDVQNLLEHKTHVLELLVGSGKQQVLIKDVQFDHLGLKPIHVDFARVSMTERVEVS
                     VPLDYRGTPVGVTEGGTFDAAIVDIEIECLVTEIPESIRVNVADLKIGDFLHVKDLVL
                     PEGVKPVTPGETIICSVRAKAAEEEVVAAAEAEEGPAEPEIIGRKEKAPEQEEESESK
                     "
     gene            complement(2586..3578)
                     /locus_tag="P5316_19975"
     CDS             complement(2586..3578)
                     /locus_tag="P5316_19975"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRR87295.1"
                     /translation="MTYDTQPDFVDCVGDAGLRVFGGTANPLLAQEIADALDVKLGQA
                     KVDRFPDGETIIKLEDDVRGRDCFLVQSTCPPVNENLMELLIFIDCLRRASARRITAV
                     IPYFGYARQDRKAEGRTPITAKLVANLISQAGAHRVLALDLHTDQLQGFFDLPVDHLS
                     AMPVIADHVAGLKLKDCTVVSPDVGNVKQATAYATRLGMELAVIDKRRLGGDSTVAAR
                     VIGDVRGKMVLLFDDMITTAGTAVEAIRILRENGAKRFLLAATHPVFAGSAPSRIKAA
                     AVDQVVVTNTIPVREELRKDLPQLTVLSVAPMLAEAIRRIHLNMSVSALFGPKR"
     gene            complement(3575..4804)
                     /locus_tag="P5316_19980"
     CDS             complement(3575..4804)
                     /locus_tag="P5316_19980"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRR87296.1"
                     /translation="MSRPTAAIILAAGRSTRMVTDLPKVLHEVCGRPMLAYVVDACRA
                     AGIERIICVVGYQKEAVIQAFRDDRQIIWVEQTEQKGTGHAAMVCRDALADFDGNLVV
                     IAGDMPLVRSQTLALLIQRHEKERSAVTLATAVLDNPTGYGRIIRDEYGNLEGIVEEG
                     DCTPEQRKIREVNPSYYCFDKKLLFTALDQVRPDNVKGEYYITDALEILIKAGHRAVA
                     VTAVPAEDAMGINSRHNLAEVGRIMQDRIQRNLMNSGVTIVDPRTTWIDVRAEIGQDT
                     VIYPFTYINGRVKIGRHCVIGPFAYLRDGTVLKDDVVAGVFIELKNSLLDEGTRARHL
                     SYIGDAEIGKRVNVGAGTIFANFDGKEIHRSYVGSETYIGNGSILVAPLAIEAGRQIP
                     HGSVVRGPESSSRDGGQ"
     gene            complement(4898..4969)
                     /locus_tag="P5316_19985"
     tRNA            complement(4898..4969)
                     /locus_tag="P5316_19985"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4934..4936),aa:Gln,seq:ttg)
BASE COUNT          963 a         1877 c         1480 g         1053 t
ORIGIN      
        1 cagtttcatc cggtcacctc acgcgtgtcc attcattcta ccccaacagg gccgggattg
       61 caccgctgcg tatgggcaat cagtctctga ttctgcaccg cttgtcagca gaacgcgctc
      121 gcgtcctcag ccccctgttg attcatcact tgcgcatgcc tgcgccaaac actcacaagc
      181 ctttcccgcc ccacgaccga ccgtcaatca cggtcggatg ggcgggcttg ccccgggttc
      241 agcagaccgc ctcgccccgc cgcgtggagc gtcagaacgg gatgtccggg ttttccaccg
      301 gcgggccgaa atcgtccggc ggcggcccct cttccaccac actggctgcc ggtctgtccg
      361 caggccggcc tgcggctcca gcaccgctgc cgaggaaagt gaaattctct accactacat
      421 cgagcttgtt ccgactctgt ccttccttgc tggtccattg ccgaaagcgc aaccggcctt
      481 caatcagtat cccgctgccc ttcttcatgt actggttgat ggtctctgcc tggcgaccgt
      541 atgaagtcac atcgacgaag cagacctcgt ctttctggtt gccatcccgg tcccgccacc
      601 ggcgattgac cgccaagccg aactcacaca ccgccgtatt gttcggcgtg taggaaagct
      661 gcggatcgcg cgtcaggtta cccgcgagga tcacccgatt gaagctggcc atgactcatt
      721 cctccgtttc ggcaggcgtc tcctccgtca cgtccggcac cgtgacctcc gactcgcgac
      781 gccgcgaacg gcgcccctcg ccggaccgat cgctgcggtc gtcatcaggg gccgcatgac
      841 tcgccggctt ggcggcggcc tccttcatct cgtcttcggt catgtgatca acccgcacaa
      901 tgaggcatcg cagcgcggcc tccgaaagct gcacatcgcg ctcgatatcc gcgatcctcg
      961 acgaatccgc ctcaaagtac gtcaggacat agataccccg ttttcgcccg cggatctcgt
     1021 aagccaggcg acgctcgtcc cacttcgcgc aggcaatcac ccgagccccg gcccggtcga
     1081 acatgcggtt gatctcgccc tgaatggcct cccagtcaga catgacgacc gggtcaaaca
     1141 ggaacattcc ttcgtatttt cgcaacaagg tcctgcacct cccacggtcc cgccggcgcg
     1201 gcccgctgcc cgccgtttca ctcctcggga cgattgaatc tgttcatcgc actgtcaata
     1261 ccttcagtga gccaacactc caccgcgtcg gccgcccggg caatggcctg ctcgacccgc
     1321 ggttgttcca ccgccgtgaa ctcgccgagc acatggttca ccatccggct gccttccacg
     1381 gattcgatac cgatcctcag tcgggcgaaa tcctcgccgc ccgcctgctc gatcacgctg
     1441 atcaacccgt tgtgtccccc cgccgagccc ccgggcctga gccgaagccg acccaacggg
     1501 agagccatgt cgtccagcac caccagcagg tcgccccgtg acagcttgtg aaacgtcagg
     1561 gcctcccgca ccgagcgccc gctgagattc atgtaggtca tcggcttgag cagaagcacc
     1621 ttgtggtccc ggatcgaacc gctgccgcac aggccgctga aacgaccgcg ggtcacctcg
     1681 atttgccagc ggcgggacag ctcgtcgacc gtccgaaacc cgacgttgtg ccgggtatgc
     1741 tcgtatttcc cgcccgggtt gcccaggccc acgatgagtt tcatcatcac agcccgtgaa
     1801 gcgtctgctc atcacgcaat cacttcgact ccgattcctc ctcttgctca ggcgcctttt
     1861 ccttgcgacc gatgatttcc ggctcagccg gaccttcctc cgcctccgcc gcggcaacga
     1921 cctcttcctc tgcggccttg gccctcaccg aacagatgat ggtttcgccc ggcgtgacgg
     1981 gcttgacccc ctcgggtaag acaagatcct tcacgtgcaa gaaatcgccg atcttgaggt
     2041 cggccacgtt cacgcggatg gattcgggga tctcggtgac caggcattcg atctcgatgt
     2101 cgacgatcgc ggcgtcgaac gtcccgccct cggtcacgcc cacgggcgtg ccgcggtaat
     2161 ccaaaggcac ggacacctcc acgcgctcgg tcatactcac gcgggcaaaa tccacatgaa
     2221 tgggtttcag tccgaggtgg tcaaactgca cgtccttgat cagaacctgc tgcttgccgc
     2281 ttccgacgag cagctcaagt acgtgggtct tgtgctccag cagattctga acatcgtgag
     2341 ctgacacgac caggttctcc ggtcgctgtc cgtgcccata caggacggcc ggaagctttc
     2401 ccgcctttcg aagacgacgg gccaccgccg acccgacgcc ttgccttgtc tcagctttca
     2461 gttgtgctat ctccatcgtc gcctcactct ttcatgccag ccgctcgaag accgctggtc
     2521 cgttttccat tgctccgcgg ctcgccgcct gacgccctat cgggtcgagc cgctcgatcg
     2581 gtttctcatc gcttcggtcc aaagagggcg ctcaccgaca tattcaggtg aatccggcgt
     2641 atcgcctccg ccagcatcgg ggccactgag agcaccgtga gttgcggcaa atcttttctc
     2701 agctcctcac ggaccggaat cgtgttcgtt accacgacct gatcgacggc cgcagccttg
     2761 atccggctgg gcgccgaacc ggcgaaaacc ggatgcgtcg ccgccaggag gaaccgcttg
     2821 gccccgttct cgcgcagaat gcgaatcgcc tcaacggccg ttcccgcggt ggtgatcatg
     2881 tcgtcaaaca gcagaaccat cttgccccga acgtcgccga tgacgcgggc cgccactgtc
     2941 gaatcgcccc ccagtcgccg cttgtcgatc accgccagtt ccatccccaa cctggtcgcg
     3001 taggcagtcg cctgtttgac gttgccgacg tccggggaga ccaccgtgca atccttcagc
     3061 ttcaggcccg caacgtgatc agcgatgacc ggcattgctg aaagatggtc caccggcagg
     3121 tcgaagaagc cctgcaactg atcggtgtgc agatcgagcg ccagtacgcg gtgggcgccc
     3181 gcctggctga tcagattggc caccagcttg gcggtgatgg gcgttcttcc ctcggccttg
     3241 cggtcttgcc gggcgtagcc gaaatacggg atcaccgccg tgatgcgcct cgcggaggcc
     3301 ctccgcagac agtcgatgaa gatcagcagc tccatcaggt tctcgttgac cggcgggcag
     3361 gtcgactgga ccaggaaaca gtcacgtccc cgaacatcgt cctcgagctt gatgatcgtc
     3421 tcgccgtcgg ggaagcggtc caccttcgcc tggccgagct tcacgtcgag cgcgtcggca
     3481 atctcctggg ccaatagcgg attagccgtc cccccgaaga cgcggagtcc ggcgtctcca
     3541 acgcaatcca cgaaatcagg ctgcgtgtcg taggtcattg tccaccatca cgacttgaac
     3601 tttcggggcc ccggaccacc gaaccgtgcg gaatctgccg ccctgcctca atggccagcg
     3661 gagcgaccag gatcgagccg ttcccgatat acgtctcgct gcctacgtag ctgcggtgaa
     3721 tctccttgcc gtcgaagttg gcaaaaatcg tgcccgcgcc cacgttgacg cgtttcccga
     3781 tctcggcatc accgatataa ctcaggtgtc tggctcgcgt gccctcgtcc aaaagcgagt
     3841 tcttcagctc aatgaacaca ccggccacga cgtcatcctt cagcaccgtc ccgtcccgca
     3901 gatacgcaaa cgggccgatc acgcagtgtc tgccgatctt gacccgcccg ttgatgtagg
     3961 tgaacggata aatgaccgtg tcctgcccga tctccgcccg aacatcgatc caggtcgtgc
     4021 gggggtcgac gatggtgacg ccactgttca tcaggttgcg ctgaatgcga tcctgcatga
     4081 tgcggccgac ttcagccagg ttgtgacggc tgttgatgcc catggcatct tcggccggga
     4141 ccgccgtcac cgccacggcc cgatggccgg ctttgatcag gatctccagc gcgtcggtga
     4201 tgtaatactc gcccttgaca ttgtccggac ggacctgatc cagagccgtg aaaagcagct
     4261 tcttgtcgaa gcagtagtaa cttggattga cttccctgat tttacgctgc tcaggcgtgc
     4321 aatccccttc ttcgacgatg ccttcgaggt tcccatattc atcccggatg atccggccgt
     4381 aacccgtggg gttatcgagc acggccgtcg cgagggtcac cgccgatcgc tctttctcgt
     4441 gacgctggat cagcagagcc agcgtctgac tgcgaaccag cggcatgtcc cccgcgataa
     4501 ccaccaggtt gccgtcgaaa tcggccaagg cgtcccggca caccatcgcc gcatgaccgg
     4561 ttcccttctg ctcggtctgc tcgacccaga tgatctgccg atcatccctg aaagcctgga
     4621 tcaccgcctc tttctggtaa cccaccacac agatgatccg ctcgatcccc gcggcccggc
     4681 aggcatcgac cacgtacgcc agcatggggc gcccgcaaac ctcgtgaagc accttcggaa
     4741 ggtccgtcac cattcgggtc gatcgacccg ccgccaagat gattgccgca gtcggtctgc
     4801 tcatgacggt atctctcaag gcgccccacc cgggtctctc gtccgcgttc gacgagacac
     4861 tccctgcttg tccgagacaa ggccgccagt tcactgacta cccggcctgg actcgaacca
     4921 ggaatgccag gaccaaaacc tggtgtgtta ccgattacac caccgggtat ccgctcggcc
     4981 cggttcgcgg gtccgaccca aaaacacaac acgctgccac gaaagatctt acggacagat
     5041 cgggcgcagc tacaggctga ccgcacgcga ggcctgagcc ccctgctgcg aagcgggggc
     5101 ccattctccc gacgccggag acgccccaaa gggtgcggcg gcagttcttc ccttcggccc
     5161 agaggccaat acgatgaaga taccttagcc acgcgccagc gtcaatagcg tcgggcacct
     5221 ccgagtgaag gcgtcgcgga gcccgtgaca gttcaggcgg ggataggcta cggctttttc
     5281 agaaccgcga ccgtgaggga gcggccccca tgtggtattg gccgctctgc ggcttggccc
     5341 ctcgcttgcg ctcggggttc tgaaagagcc gcc
//
LOCUS       DAOTPW010000322         5339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12908_length_5339_cov_5.274981, whole genome shotgun sequence.
ACCESSION   DAOTPW010000322 DAOTPW010000000
VERSION     DAOTPW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12908_length_5339_cov_5.274981"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="P5316_19990"
     CDS             <1..1294
                     /locus_tag="P5316_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87297.1"
                     /translation="AVAELAVAVLGSDLLTNGDFENGETGWTKWQADWSTGLEWDFNN
                     PNVSSMGGTKCLRLGKETTLASFGVYQQVAVTPGKTYRLDCQWKGKRLGTMNWWEVIL
                     IDGPFSLQQADDAEYVEKNYMFAYDNMTYGFPGAIGTGFGWVWTHNQYAPPKDQVDWN
                     HRLGRRKATGNIMTVVLKAGSNTAGGVEAYFDNVTLREVSEDYVLDAVSNPTWGAQLA
                     ADMSSLPVGQYPGELRVTIYDAMLNESSIYRNVHLQPIPLNPWVCKNRDALYQEVFVG
                     DPIPADNVEIWNCGGEGGDLSYEITTNVPWIHVVPDEGVSVQNPPTTNLHVVSYDPLP
                     PGVHTGKITITGSHNVETINVTVVVTTVKPDFDADGDVDETDFGFFQQCYTGAAQVSG
                     SCTVADFDGDMFVTRTGDLPVFLNCLSGPGIYPDRDCD"
     gene            1798..3687
                     /locus_tag="P5316_19995"
     CDS             1798..3687
                     /locus_tag="P5316_19995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR87298.1"
                     /translation="MKRMTSFSEALFARRMTSVLVLVAGGCQWFSESADRDVYRLIND
                     RQRAALGEAHDFRIERGTSPVSVPGDAYAFVPRPIDSDVPEVFRRAASQPAVDGNPTS
                     APVVMGSQGYVFPETMPSGTADAEASQPGLLRSEAASRPVSTRPSRPIMTLSGALKYA
                     FQHSRRFQNAKEDLYLAALDLTLERHLWTPRFFGEIGVDLVNQGQDEVWDHALEAVAQ
                     AGVRQKLPYGGEITARVIANLVNDLSKRVNEGETGSMLLEANIPLLRGAGRAAYESRY
                     RAERNLIYAVRTYEQYRRSLAVDIAGRYFNLQRLRQQIVNASESVEQFATDMARAEAL
                     RRVGRVEQLDVLRADQDRLRAINTEVDAIANYETALDRFKIEIGMPTETEIDVEYPAE
                     VTDSTTEMDGVMEPSSLEDALRMPDVSEEEAVRVALKYRLDFLNDLDRVDDARRGVAV
                     AENNLLPDLRMYGTVQLDTVPERVGVLKYNTDRTGYRTGLRLDLPLDRVAERNALRKS
                     QIALQQAQRALEEAKDIISLQVREAIREVQQQADSLRIQILSRDQALARRRAARLWFD
                     LGKMNNRDVIEAQRELLEAQNRLAEAQSQWRLAILAFRLDTGTLRVDDEGQWYSAVAT
                     TAARR"
     gene            3774..>5339
                     /locus_tag="P5316_20000"
     CDS             3774..>5339
                     /locus_tag="P5316_20000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR87299.1"
                     /translation="MGMTETEATLPSQVPEAAEQAWAPSRVPVRVSRRALRWLVLIVV
                     AVAAIGAYAVVKRWLAPAAVQDIQLYTVTRKSFPILLTATGELKAANSVEVRSEVEGR
                     ATIISLVPEGTHVKQGDLLVELASDEIDEKIRDLEIKETVAAAAYEAAVKELEILKDK
                     NQSEERKAALALDMARLSLEKYRKGESKELRKESEIAVKQAEQELQRAKDYLKDSEEL
                     FKEGFITRIERERDEFEAWKAENVLERTGVKAEVLEKWTIPMELQKKESDVEEAEAEL
                     ERTKKASRASEEKAIAEVNAKKDELGIVRDKLNKAREQKKKTRIEAPADGLVVYYKED
                     WWDETRIKTGTQLHERQQIIELPDTSSMKVVVRVHETQIERLKEGLTATITLEGYSGR
                     QYPGKVIKIGTVADSAHRWMNPNTKEYLTEILIEGSTSDLKPGGTAKVDVLVTQLSDV
                     VAVPVQAVFGKGGKYYVFVDERGNARPVEVEVGLASSEYVEIKKGLDVGQEVRLAITD
                     EMKRRLPDDKEKEE"
BASE COUNT         1113 a         1424 c         1789 g         1013 t
ORIGIN      
        1 tgccgtcgcc gagctggccg ttgccgttct gggttcagac ctgttgacca acggtgattt
       61 cgagaacggt gagacaggtt ggaccaagtg gcaggccgat tggagcacag gtttggagtg
      121 ggacttcaac aaccccaacg tcagctcgat gggtggaacc aaatgccttc gccttggaaa
      181 ggagacgacc cttgccagtt tcggcgttta tcaacaggtt gccgtcacgc ccggcaagac
      241 ctatcgattg gactgccagt ggaagggcaa acggctgggt accatgaact ggtgggaggt
      301 catcctgatc gacgggccct tcagcctgca gcaagcggat gacgccgaat acgtcgagaa
      361 aaactacatg tttgcctatg acaatatgac ctacggtttc cccggcgcga tcggcacggg
      421 cttcggctgg gtctggacgc acaaccagta tgccccaccg aaggaccaag tggactggaa
      481 tcatcgcctg gggcgtcgta aggcgacggg gaacatcatg acggtcgttc tcaaggcggg
      541 cagcaacacc gcgggcggcg tcgaggcgta ctttgacaac gtgacgcttc gagaagtgag
      601 cgaagactat gtgcttgatg ccgtttccaa cccgacgtgg ggggcgcagt tggcggcaga
      661 catgagttca ctgccggtcg gccagtatcc gggcgagctt cgggttacca tttacgacgc
      721 gatgctcaac gaaagcagca tttaccgcaa cgtccatctg cagcccattc cgctcaatcc
      781 gtgggtttgc aaaaacaggg acgccttgta tcaggaggtt ttcgtcggag atccgattcc
      841 cgcggataac gtggagatct ggaactgcgg cggtgagggc ggcgatctga gctacgagat
      901 caccaccaat gtgccgtgga tccacgttgt gccggatgaa ggcgtgtccg ttcagaatcc
      961 gccgacgacc aatcttcatg tggtttctta tgatccgttg ccgccgggtg tccacacggg
     1021 caagatcacc ataaccggat cgcacaatgt cgagacaatc aacgtcacgg tagtggtgac
     1081 cacggtcaag ccggactttg acgccgacgg cgacgtggat gagaccgatt ttggtttctt
     1141 ccagcagtgc tacaccgggg ccgcgcaggt ctccggcagt tgtaccgtcg cggacttcga
     1201 cggcgacatg ttcgttactc gtaccggtga cctgccggtg ttcttgaatt gcctgagcgg
     1261 acctggcata tatcccgatc gggattgcga ctgagtgcga gtcgggacgc tcctcgtgta
     1321 aagataacgg cgtgcgaagc tcttgggatg ctccgcacgc cgttcttgtt gtgctttcgg
     1381 cccgggcggg ggagatcggc tatgcgcgca acggtcggac ggatccgtat ccggctgaga
     1441 gcgcctcaag gacaagcggc agcccggatg tcgaagcctg tgcgcgtgac gcgccagcag
     1501 gtggttgcgg catcatagag acggatttcc cgcactctcc gtcggtcatt cccagatggc
     1561 gtgtttcgat tggcgggctg cgggttttgc cccgcctgcc tttttgccgt ttggcgattc
     1621 aatcccacaa tggtttgcat tagaatgcgt ccgcgacgcg tttctgctgt atgagttctc
     1681 agggcataca actgcctgcg ccgagcgtct ttatgcgcgg gccaagacgc aggcgaggca
     1741 tcggaatata ccagaggcct gccggcttgc ttaagcatcc cggacgatga gactgttatg
     1801 aagcggatga cgagtttctc tgaagccctg tttgcccgcc gaatgacgtc agtccttgtc
     1861 ttggttgccg gaggatgtca gtggttctcc gagagtgcgg accgtgacgt ctatcggttg
     1921 attaacgacc gccagcgggc ggctctgggc gaggcgcatg acttccgcat tgagcggggc
     1981 acctcgcccg tgtcggtgcc gggagacgcg tacgcgttcg ttcctcgccc gatagacagc
     2041 gacgtgccgg aggtttttcg tcgtgcggct tctcaacctg cggtggacgg gaacccgact
     2101 tcagcaccgg tcgttatggg ctctcagggg tatgtatttc cagagacgat gccttcgggg
     2161 acggcggacg ctgaggcgtc ccagcctggc cttttgcggt ctgaagccgc cagccggccg
     2221 gtgtccaccc ggccgtctcg acctatcatg acgttgtctg gagccctcaa gtacgccttt
     2281 caacactcgc ggcggtttca gaatgccaag gaggatctgt accttgccgc cttggatctg
     2341 accctggaga gacatctgtg gacacctcgg tttttcggag agatcggagt tgacctcgtc
     2401 aaccagggac aagacgaggt ttgggatcat gccctcgaag cggtcgcaca ggcaggggtt
     2461 cgacagaagt tgccttacgg cggcgagatc acggcgcggg tgatcgccaa cctggtcaat
     2521 gacttgtcca agcgcgtgaa tgagggtgag accggctcca tgctcctgga ggccaatatt
     2581 ccgttgctgc gtggagccgg ccgggcggcc tatgagtcgc gatacagagc cgagcgcaac
     2641 ctgatctatg cagtccggac ctatgagcag tatcgtcgat cattggccgt ggacatcgcg
     2701 gggcgctact tcaatcttca gcggcttcgc cagcaaatcg tcaacgcgag cgagagtgtc
     2761 gagcagttcg cgaccgacat ggcccgcgcc gaggctcttc gtcgggtcgg ccgcgtggaa
     2821 cagttggacg tgcttcgtgc cgatcaggat cgcctgcggg ccatcaacac ggaagtagac
     2881 gcgatcgcca attacgaaac cgccctggac cgcttcaaga tcgagatcgg tatgccgacg
     2941 gagaccgaaa tcgacgtgga atatccggcc gaggtcaccg attccacgac ggaaatggat
     3001 ggagtcatgg agccttcgag tctggaagat gctcttcgaa tgccggatgt gtcggaagaa
     3061 gaggccgtcc gcgtggctct gaaataccgg cttgattttc tgaacgacct ggaccgcgtg
     3121 gacgatgctc gccgaggcgt ggccgttgcc gagaacaatc ttctgccgga tctcaggatg
     3181 tacggcacgg tgcaactgga caccgtgccc gagcgtgtgg gcgtgctgaa gtacaacacg
     3241 gaccgcacag gttatcggac gggcttgaga ctggatctgc cgttggatcg tgtggcagag
     3301 cgcaacgcgt tacgtaaatc tcagatcgcg cttcagcagg cgcaacgggc gcttgaagag
     3361 gccaaggata tcatcagcct gcaggttcgt gaggcaatcc gagaggtcca acagcaggcc
     3421 gattcgctgc ggattcaaat actgagccgg gatcaggcct tggctcgccg ccgggcggcg
     3481 cgtctgtggt ttgacctggg aaaaatgaat aaccgggacg tcatcgaggc gcagcgagag
     3541 ctgcttgagg cacagaatcg cctggcggag gctcagtccc aatggagact ggcgattctc
     3601 gcttttcgtc tcgataccgg aacgctccgc gtggacgacg agggccagtg gtactcggcg
     3661 gtggcgacca cggccgcgcg gcgttaaaat ggaagcggtc aacgtccttc cgaatggggt
     3721 gcgacaagtt tccgggccgc cgacggtcag cctgcttgtt aggagatccg ggcatgggaa
     3781 tgaccgagac cgaagcgact cttccgtcgc aagttcctga ggctgcggaa caggcctggg
     3841 cacccagccg ggtgccggtc cgagtttcgc gaagggcgct ccggtggctt gtgctgatcg
     3901 tcgtggcggt tgccgcgatc ggggcttatg cggtggtcaa gcggtggctc gctccggccg
     3961 ccgtgcagga catccagctt tacacggtca cgagaaaaag cttccccatc ttgttaaccg
     4021 ctaccggcga gctcaaggcg gcgaattccg tggaggtgcg gtccgaggtg gaaggccggg
     4081 cgacgatcat ctcgctcgtt cctgagggga cgcacgtgaa gcaaggcgac cttctggtgg
     4141 aactggccag cgacgagatt gacgagaaga tccgcgacct ggagatcaag gagacggtgg
     4201 cggcggccgc ctacgaggcc gcggtgaaag agttggagat tctcaaggac aagaaccaga
     4261 gcgaggagcg caaagccgct ctggccctgg acatggccag gctgagtctc gagaagtatc
     4321 gaaaaggcga atccaaggag ctgaggaagg aatccgagat cgccgtcaag caggctgagc
     4381 aggagttgca gcgggcgaag gactacctca aggactcgga agagcttttc aaagagggtt
     4441 ttatcactcg catcgagcgg gagcgggacg aattcgaggc atggaaggcc gaaaacgtac
     4501 tggagaggac gggggtgaag gccgaggtcc tggagaaatg gacgattccg atggaactcc
     4561 agaagaagga gtcggacgtg gaggaggccg aggccgaact ggagcgcacc aagaaggcgt
     4621 cgcgggcctc tgaagagaaa gccattgccg aagtgaacgc gaagaaggac gagttgggga
     4681 tcgttcgaga caagctcaac aaggcccgag agcagaagaa gaagacgcgg atcgaggctc
     4741 ccgccgatgg gctggtggtc tactacaagg aggactggtg ggacgagacc cgcatcaaga
     4801 cggggactca actgcatgaa cggcagcaga tcatcgagct accggacacg agctcgatga
     4861 aggtggtcgt gcgtgttcac gagacgcaga tcgagcggtt gaaggagggg ttgaccgcga
     4921 cgatcacgct ggaagggtac agcgggcggc agtatccggg gaaagtgatc aagatcggca
     4981 cggtggccga ctcggctcac cggtggatga accccaacac gaaggagtac ctgaccgaga
     5041 ttctcatcga gggttcgacc agcgatctca agcccggcgg cacggcaaaa gtggacgtgc
     5101 tggtgacgca gctcagcgac gttgtggccg ttccggtaca ggcggttttc ggcaaggggg
     5161 gcaagtacta tgtgtttgtc gatgagcgcg gcaacgcgcg tccggtcgag gtcgaggtgg
     5221 gactggcgag cagcgagtac gtggaaatca agaaggggct ggatgtcggt caggaggttc
     5281 gtcttgcgat caccgatgag atgaagcggc ggcttccgga cgacaaagag aaagaggag
//
LOCUS       DAOTPW010000323         5326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_12942_length_5326_cov_5.003984, whole genome shotgun sequence.
ACCESSION   DAOTPW010000323 DAOTPW010000000
VERSION     DAOTPW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12942_length_5326_cov_5.003984"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="P5316_20005"
     CDS             <1..1090
                     /locus_tag="P5316_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87300.1"
                     /translation="PGQASVPVAWPFICQSYETRTTAPFCPPLQTNVRTAIAVGMNAL
                     LDNNPCHCEDAANQKPTNYHLSFYDGLKWRILHPSHPGPSGENAQWSTTAEGKYFVLG
                     AKINTVTMIVKTNTVDITMYAKNTPMGDGTYEAVTSTVTGLKRQYLGHFNKLHAGASI
                     GCRMDSNGACIGNRTCLKSEYDRCDGLGKLAWGAKWLVYDGIALSGGMGYGAPGACCL
                     PGGGCGVTEPGVCDGAGGQFVGPNTTCGSSTCQGACCKPTGECADNTYFDACPGTFQG
                     FGTDCATTVCPCPTPFADADADQDVDQADFARFQACFTGPGSESVTGLCRCFDRDNNG
                     QGDGDVDMDDFSAFEACASGPGIPTACQ"
     gene            1286..3046
                     /locus_tag="P5316_20010"
     CDS             1286..3046
                     /locus_tag="P5316_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87301.1"
                     /translation="MKRRSLPILATLCLVLSVEVLAETWCSSAYPTDPPACRCSAIWC
                     EDLDRICNGAPACPPAPPEPCTEDQGWSWSLIRQAFPRTSRINDTGDLCGVEFKGEDG
                     GDWVSSPPLAARFPKGQLGQATVDLIDDIQFKWGSSYDRVIGTDQFPLVLRFDMCGGN
                     EEGEVISGVHVQWDIGVMELFLDTGLPNGNRSPMDYILVGLEEDPVNKPGCVSCYKTC
                     PEGWTNVHNPWPHVCQSYEPRSEAPACPPLQTNVRTAIAIGANALLDNNPCHCQAPET
                     PAEDRVREVPTNRHLSFYDGLKWRIINPDHPGPGGETLAWWTDHPHPNNSDYFVLGEK
                     TNEIILTVKTGTVDITHRTRIRIDGQLQWVESRVTGISRKYVGPFNKLHAGASIGCRM
                     DSSGHCVGNRSCIANAYDTCNSSGKPEWGARWLSFDNLSLRGGLPDGQGACCFGGGNC
                     AVGTPVACEGAGGRYAGPDTTCEDCRGACCLPSGECQDTDFDACPGVFRGFGTDCATT
                     VCPCPTPFADADADRDVDQADFARFQACFTGPGSESVTGLCRCFDRDNNGQGDGDVDM
                     DDFSAFEACASGPGIPTGCQ"
     gene            3225..>5326
                     /locus_tag="P5316_20015"
     CDS             3225..>5326
                     /locus_tag="P5316_20015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87302.1"
                     /translation="MRQRKFSRHLACFIVVPTLLVPGASLKAQFNDVQTFDGTVGSIF
                     SATYWTIDGGCSGAVVFRDTKRGDEGSKCLRSLHPVGVGTRMIRDLNPGPGSSIVGQT
                     AVSGIEEVVRLWISADDGSGATGQEFYRVQGALYPVTSVEMDINPGAAGSNPANLFRQ
                     EPGEQGNVYFSADDGVNGRELWLCHTTTGLVTTTYLLADIHAGPGSSDPGGFAVVGNN
                     MIKGVVFAATDETHGRELWFSDGITTTLLKDINTASAGASSEPQLLNTLLISWPWSGP
                     YDAKVFFTADDGVHGRELWRTDGTLAGTGLVKDIVAGPAGSNPASLLPVKLYAGSQLY
                     PSALFTVNSDGVNGEELWKTGGALDGSDTVMVYDPGPAGSPKNLITAGALYKGFFTVG
                     NSLWRTDGTAGGTYVVKTFASPPSNFVAVGDKFYFTADDGTGKGVELWVSAGTSSYTK
                     LVKDINPGTAGSSPANLTAVGSNLLYFSAIDGTHGRELWKSSVSSDWLTFPTSMVQDI
                     NPGAAGSEPGNLTAFDGTLIFTADDGIHGRELWQTDGLAAPNYSGMSYKWDLSSLIQS
                     KGDPARIAVAGTDEAPLSVYLLYDFPAMGGLDLGMSEAAHQVNTYVELADGADRAPLA
                     ITYVDCGDGNLPRPRAALTDGGNHNAIAFGMVAVLDQNPCDTDPRGGGSPGVPFAYAP
                     AVYDGRDWITMDLAHVPAG"
BASE COUNT         1010 a         1553 c         1716 g         1047 t
ORIGIN      
        1 ccccggacag gcgagcgtgc cggtggcgtg gccgttcatt tgtcaatcct atgagacgcg
       61 gaccacagcg ccgttctgtc ccccgttgca gactaatgtg cggacggcca tcgccgtggg
      121 catgaacgca ctgctggaca acaatccctg tcattgtgag gacgcggcca accagaagcc
      181 cacgaactac catctgtcgt tctatgacgg cctgaagtgg cggattctcc atccgagtca
      241 tcccggcccg agcggagaga acgcacagtg gtcgacaacg gccgagggca agtatttcgt
      301 gctcggagcg aagatcaata cggtgacgat gattgtcaag accaataccg tcgacattac
      361 gatgtacgcc aagaacactc cgatgggcga cggcacatac gaagcggtca ccagcacggt
      421 gacagggctc aaacgccagt accttgggca tttcaacaag ctgcatgccg gtgcctccat
      481 cggttgcaga atggacagca acggcgcttg cataggcaac cggacgtgtc tcaagtccga
      541 gtacgacagg tgcgacggat tgggtaagct ggcttggggc gccaagtggc tcgtgtacga
      601 cggcattgcc ttgtccggtg gcatgggtta cggcgcgccg ggtgcctgtt gcctgcctgg
      661 cggaggatgt ggggtcacgg agcccggcgt gtgcgatggt gcaggcggcc agttcgttgg
      721 tccgaacacg acatgcggtt cgtcgacgtg ccagggagct tgctgcaagc cgaccggcga
      781 gtgtgctgac aacacgtatt ttgatgcctg tccgggcacg tttcaggggt tcgggaccga
      841 ctgcgcgacg accgtctgtc cgtgcccaac gcctttcgca gacgcggatg cggaccagga
      901 cgtggaccag gcggacttcg ccaggtttca ggcatgcttc accgggcccg gttcggaaag
      961 cgtgaccggc ctgtgcaggt gctttgatcg cgacaacaac ggccaaggcg acggcgacgt
     1021 cgacatggat gatttcagcg ccttcgaggc ttgcgccagc ggacctggca tacccactgc
     1081 gtgtcagtga ggtgtcaacc tgcggaaaca cctcgccgtg gttactgcca accggcgtgt
     1141 tgcctgcgga cccttcacag gcggacgttc ttcggtgttc gttggatacg gagggccttc
     1201 gcgacctcgg tgatgaggcg actccgacct gagtcgtcct tgtgtcgtgc gcgccggtcg
     1261 cccacggccg aaaaggagcg ctgagatgaa acggcgatca cttccgattc tcgcgacact
     1321 ctgcttggtg ctttcggttg aggtgctcgc cgagacgtgg tgcagcagcg cctatcccac
     1381 ggatccgccg gcatgtcgtt gttcggctat ttggtgcgag gatctcgacc ggatctgcaa
     1441 tggcgcgccg gcctgtcctc ctgctccgcc tgagccttgc acggaggatc agggctggag
     1501 ttggagcctg atccgtcaag cctttccgcg cacgtccagg atcaacgaca cgggtgactt
     1561 gtgtggcgtg gagttcaaag gggaagatgg gggtgactgg gtcagttctc cgccgctggc
     1621 cgccaggttc cctaaagggc aactcggcca ggcgaccgtt gatctcatcg atgacattca
     1681 attcaaatgg ggatcctcat acgacagggt aatcggcact gatcagttcc ccttggtgct
     1741 tcgctttgac atgtgcggag gcaacgaaga aggcgaagtc atttccggcg tgcacgtaca
     1801 gtgggacatc ggcgtgatgg agctgttcct ggacaccggg ttgcccaatg gcaaccgttc
     1861 gccgatggat tacatcctgg tcggcttgga ggaagatccg gtcaacaaac ccggttgcgt
     1921 cagttgctac aagacatgcc cggagggttg gacgaacgtg cacaacccct ggccgcacgt
     1981 ttgccaatcg tatgagcccc ggagcgaggc accggcctgc ccgccgctcc agaccaacgt
     2041 gcgcacggca atcgccatcg gagccaatgc cctattggac aacaatccct gtcattgcca
     2101 ggctccggag acccccgccg aggatagggt gagggaggtt cccacgaatc gccatttgtc
     2161 tttctatgac ggcctgaaat ggcggatcat caatccggat cacccggggc ccggaggcga
     2221 aaccttggca tggtggacgg accatcccca tcccaacaac agtgactatt tcgtcctcgg
     2281 ggaaaagacc aacgagatta ttctcacggt caagaccggc accgtggata ttactcaccg
     2341 aacgagaatc aggattgacg gccagttgca gtgggttgaa agccgagtca cgggcatttc
     2401 acggaagtac gtcgggcctt tcaacaagct ccacgcgggc gcctcgatcg gctgcagaat
     2461 ggatagcagc ggtcattgcg tgggcaacag atcctgtatc gcgaacgcat acgatacctg
     2521 caacagctcg ggcaagccgg aatggggtgc caggtggctg agttttgaca accttagtct
     2581 gcgcggcggg ctgcctgacg ggcagggagc atgttgcttt ggtggcggca actgtgccgt
     2641 gggtactccc gtggcctgcg agggtgccgg cgggcgctat gccggtcccg acaccacatg
     2701 tgaggactgc aggggggcgt gctgtctgcc gagcggtgag tgtcaggaca cggatttcga
     2761 tgcatgtccc ggcgtgtttc gggggttcgg gaccgactgt gcgacgaccg tctgtccgtg
     2821 cccaacgcct ttcgcagacg cggatgcgga ccgggacgtg gaccaggcgg acttcgccag
     2881 gtttcaggcc tgcttcaccg ggcccggttc ggaaagcgta accggcctgt gcaggtgctt
     2941 tgatcgcgac aacaacggcc aaggcgacgg cgacgtcgac atggatgatt tcagcgcctt
     3001 cgaggcttgc gccagcggac ctgggatacc gacagggtgc caatgagagc acatttcagg
     3061 ggagaaagcg gtctgatgcg gccaaggtgt gcaggcgcga acgttacgca gatgttcagc
     3121 aggccgaaaa gccaggcaga ggcgtttgcg gtggaacgtt gccgttgacc ggcagaaaaa
     3181 gagcagtcgg ccctggcggc catcacggaa aggagcgact cgcattgcgg cagcgaaagt
     3241 tctcccgcca tcttgcgtgc ttcatcgtgg tgccaacgtt gctggttccg ggggcaagtc
     3301 tcaaggcgca gttcaacgac gtccagacat ttgacggcac ggtgggcagc atcttcagcg
     3361 ctacgtattg gaccattgat ggggggtgtt ccggggccgt tgtcttccgc gatacgaaac
     3421 gcggggacga gggatcgaag tgtctgcgaa gcctgcatcc cgtgggcgtg ggcacccgca
     3481 tgatcaggga tctcaatccc ggccctggca gctcgatcgt cggccagacg gcggtcagcg
     3541 gaatcgagga ggtggtgcgt ctttggattt cggccgatga cggttccggc gccacggggc
     3601 aggagttcta ccgcgtccaa ggggctttgt accccgtcac gtcggtggag atggacatta
     3661 accccggtgc ggccggctcg aatcccgcga atttgttccg ccaggaaccg ggcgagcagg
     3721 gtaacgtcta tttcagcgcc gacgacggcg tgaacggccg cgagctgtgg ttgtgtcata
     3781 cgaccacggg ccttgtaacc acgacgtatc tgcttgcgga tatccatgcg ggtcccggca
     3841 gttccgaccc cggcgggttt gcggtagtcg ggaacaacat gatcaagggc gtggtcttcg
     3901 ccgcaacgga cgagacccac ggtcgtgaac tctggttcag cgatgggatc accacaaccc
     3961 tgctgaagga catcaacact gcctcggcag gcgcttcatc tgagccgcag ctcttgaaca
     4021 cgctcctcat ttcctggccc tggtccggac cctatgatgc caaggttttc ttcaccgccg
     4081 acgacggggt tcacggtcgc gagttgtgga ggactgacgg aaccctggca ggtaccggtt
     4141 tggtcaagga tatcgtggcg ggtccagcgg gctccaatcc agccagtctg ttgccggtaa
     4201 agttgtatgc cggttcccaa ctgtacccga gcgccctctt tacggtgaac agcgacgggg
     4261 ttaacggtga ggagctctgg aagacgggcg gcgccttgga cggttcggat acggtgatgg
     4321 tttacgatcc gggtcctgcg ggaagcccga agaacctcat tacggccggc gcgctctata
     4381 agggtttctt caccgtcggc aacagcttgt ggagaacaga tggcacggcg ggcggtacct
     4441 acgtggtcaa gaccttcgcc tctcctccct ccaacttcgt ggccgtcggc gacaagttct
     4501 acttcacggc ggatgacggt accggcaagg gggttgagtt atgggtgagc gccggcacaa
     4561 gctcgtacac aaagctggtc aaggatatca accctggcac cgccggttcc tcgcctgcaa
     4621 atctgaccgc cgtcggcagc aacctgttgt atttctcggc gattgacggc acgcatggcc
     4681 gcgagctgtg gaagtcctcc gtcagctcag attggcttac gttccccacg agcatggtcc
     4741 aagatatcaa tcccggcgct gccggctcgg aacccggcaa cctcaccgcc tttgacggca
     4801 ccttgatctt cacagccgac gacggcattc atggtcgcga actgtggcag accgacggtc
     4861 tcgccgcacc caattactcc ggtatgagct ataagtggga tctctcttcg ctgattcagt
     4921 cgaagggtga tcctgccagg atcgcagtcg cgggcaccga tgaagctccc ttgtcggttt
     4981 acttgttata tgattttccc gcgatgggcg gcctggatct ggggatgtcg gaggccgctc
     5041 accaggtgaa cacgtacgtg gaactcgcgg acggtgccga tcgcgctccg ctagccatta
     5101 cttatgtgga ttgcggcgat ggcaatctgc ctcgcccccg ggctgctctt accgacggcg
     5161 gcaaccacaa cgcaatcgcc ttcggcatgg tcgccgtgct tgaccagaac ccgtgtgaca
     5221 ccgatccgcg cggcgggggc agccccggcg tgcccttcgc ctacgcgccc gcggtgtacg
     5281 acggccgtga ctggattacg atggacctcg ctcacgttcc cgcagg
//
LOCUS       DAOTPW010000324         5281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_13063_length_5281_cov_5.260429, whole genome shotgun sequence.
ACCESSION   DAOTPW010000324 DAOTPW010000000
VERSION     DAOTPW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13063_length_5281_cov_5.260429"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="P5316_20020"
     CDS             <1..280
                     /locus_tag="P5316_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR87303.1"
                     /translation="QRVAIARALANDPAIILADEPTGNLDSATGSEIIDLLSSFNRQR
                     GVTIISATHDHKMLAASTRVVWITDGRVDRIERREALQIAVGSIDGRS"
     gene            complement(333..1739)
                     /locus_tag="P5316_20025"
     CDS             complement(333..1739)
                     /locus_tag="P5316_20025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR87304.1"
                     /translation="MRAHLRFHGAAKEVTGSMHLIEVNGDVICLDCGLFQGRRAEANA
                     KNRTLPCDPRDIAAVILTHAHVDHCGRLPLLVKSGFAGAIYATSATCDLAEVLLADSA
                     HIQQEDAEYWNKKRVKKGDAPIEPLYNQADVEDTVKLFRRRELRETFDVLPGIRARLH
                     EAGHMLGSCGAKVDADLGPGKTRSIVYTGDLGRKGMEILRDPDPLPECDYLICESTYG
                     GRQHSRPEQMREEFAEIVNETVARGGKLIIPSFAVGRTQVLVYEYHMCLHQGLIPKGI
                     PLVVDSPLAVRATEVFRKHPEVFDEESSAFNHLTGDMLSCDDCEYTQDVEESKRLHDR
                     KGPMIIISASGMCEVGRILHHLKNNIENPANTILIVGFQAENTLGRRLLEKAKQVKIF
                     GETYQVKARIKVLTGFSAHANSIELMAAVEPLARRLRRVFLVHGEAGQSQTLAETMRK
                     NGIADVVIPEPDQRFPIE"
     gene            2167..4482
                     /locus_tag="P5316_20030"
     CDS             2167..4482
                     /locus_tag="P5316_20030"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRR87305.1"
                     /translation="MDHAAIADFLKKNVGLFKHFPDHRVAELVAGSQVASYEPNEAIV
                     EFGEDATHLGAILEGEASASVIAESGDRQVLRRLKTGDTFGEMALMTGEKTMIDIIAE
                     TRCSVLLVPVSLFSSVIMTESRAVQHISRTMSERFRTVMLDPSQAAALRNQKKPITIQ
                     LKGERPEKILVLNCGSSSLKYCLFDTQNESNNVRGLVDRIGTDITRHTCRGPRGEVAR
                     NLPKGTHREAFEAMFAELTAKETGAVSSAAEISAVGHRVVHGGERFAETTVITDDVLR
                     ELETLCPLAPLHNPINLLGIREALRVFPSVPHFAVFDTAFHNTLPSFAYLYGLPYEYY
                     EKKKVRRYGFHGTSHAYVCLAAAQYLCRRVNELEIISCHLGNGASVCAVDHGRSVDTS
                     MGLTPAEGLIMGTRGGDIDPGVLVHLQREEGLTADQIDEILNRKSGLLGLSGISSDMR
                     EVERAAHEGNPRALVALKTFCYRLRKYIGAYVAAMGGLDVIIFTAGIGQGSARVRALA
                     LQGLRCMGVVLDEQRNREAHACEEIRRISADDSPVTVLVVPTDEERMIARECLRALSR
                     SYIKQVLETQRQEPIPIEVSAHHVHLTQEHVEALFGVGHELTRQSDLSQPGQYACQEQ
                     VTLIGPRGRVERVRVLGPVRQFTQVEIAMTEQFKLGIHPPIRESGDLKDTPGITIEGP
                     AGSVTIDKGVICAMRHIHMTPEDALRYGVRDKFTVRVCVPGDRELVFGDVRVRVDPSF
                     RLAMHIDTDEGNAANISTGMTGYIDGIQNQG"
     gene            complement(4473..>5281)
                     /locus_tag="P5316_20035"
     CDS             complement(4473..>5281)
                     /locus_tag="P5316_20035"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87306.1"
                     /translation="RTDQKGVVTTFEWDNMYRLLQRTYPSRSDTFTYDRSGRLLTANN
                     SLVNETFEYDILGRVTSLTQAYGASETYTTTVSYAIGTPDSTATVTYPASREVVYTFD
                     GRFRMTSVSGGTGVGVTRGLDLADQVISSSFANGVSSVFDYDVNGRMTHMERFKNGEP
                     PTMLVKYDYGYDAVGNRLYTRDLMATDRSELYGYDARNRLRNFARGTLNAGPRARPKG
                     AATGSNGRRQTAPAKLFGAAPPDQERTEIIANRQPAWISVPAAAPQGPHP"
BASE COUNT          930 a         1633 c         1665 g         1053 t
ORIGIN      
        1 gcagcgtgtg gccatcgctc gggccctggc caacgatccg gccatcattc tggctgatga
       61 gcccaccggg aacctcgact cggcgaccgg atcggaaatc atcgacctgc tctcgtcctt
      121 caatcgccag cgaggtgtca ccatcatcag cgccacgcac gatcacaaga tgctggccgc
      181 ctcgacccgc gtggtgtgga tcaccgacgg ccgcgtggat cgcattgagc gccgcgaggc
      241 ccttcagatc gcggtgggga gcatcgacgg gcggagctga gcggagtgcg aatcgatggc
      301 aggcaatccg tcctttgccc ggaccacaag ccctactcga tggggaatcg ctggtcgggt
      361 tcggggatga ccacgtcggc gatgccgttc ttgcgcatcg tctcggcaag agtctgcgac
      421 tggccggcct cgccgtgaac gagaaacacg cggcgcaggc gtcgtgccag cggctcgacc
      481 gcagccatca gctcgatcga attggcgtgg gcggagaagc cggtcagcac cttgatcctg
      541 gctttcacct ggtaggtttc gccgaagatc ttgacctgct tggccttttc gagcaggcgg
      601 cggccgaggg tgttctccgc ctggaagccg acgataagga tggtgttggc cgggttttcg
      661 atgttgttct tgagatggtg gaggattcgg ccgacttcgc acattccgct cgcggagatg
      721 atgatcatcg gtcccttgcg gtcgtggagg cgcttgcttt cctcgacgtc ctgcgtgtac
      781 tcgcaatcgt cgcaggagag catgtcgccg gtgaggtgat tgaacgccga ggattcttcg
      841 tcaaacacct ccggatgttt tctgaacact tcggtcgccc gcacggccag agggctgtcc
      901 accaccagcg gaataccctt ggggatcaat ccctgatgga ggcacatatg gtactcataa
      961 acgagcactt gggttcgccc gaccgcgaac gatggaatga tgagcttgcc gccccgggca
     1021 accgtctcgt tgacgatttc cgcgaattcc tcgcgcatct gttcgggtcg ggagtgctgc
     1081 cttccaccat aggtgctctc gcagatgagg tagtcgcact cgggcagggg gtcggggtct
     1141 cgcaggattt ccatgccctt gcgcccgagg tcaccggtgt agacgatcga tcgcgtcttg
     1201 ccgggtccca gatcggcgtc gacctttgcc ccgcacgagc cgagcatgtg accggcctca
     1261 tgaagcctgg ccctgattcc cggcagcacg tcgaaggtct cgcggagttc gcgccgtcgg
     1321 aacagcttga ccgtatcttc gacatcggcc tggttgtaca gcggttcgat gggggcatcg
     1381 cctttcttga cgcgcttctt gttccagtat tcggcatctt cctgttgtat gtgagccgaa
     1441 tcggccagca gaacctcggc caggtcgcaa gtggcggacg tcgcgtagat tgctcctgca
     1501 aagccgctct tgaccagcag gggcagacgg ccgcagtgat ccacatgggc gtgcgtcagg
     1561 atgacggctg caatgtcccg cgggtcgcac ggcagggtgc gattcttggc gttggcttcg
     1621 gctcgtcggc cctggaacag cccgcaatcc aggcagatga cgtccccgtt gacttcgata
     1681 aggtgcatgg aaccggtgac ttccttcgcc gccccgtgaa aacgcaagtg tgcccgcatt
     1741 ggctgcaatc ctcgccttcg accccaccgg ccttgtgcgt gtcaagtcaa tacatcactt
     1801 gtttaacttg tttaacgggt ttcttcgact ttgtccaccc gaaagcaccc aaggcggacc
     1861 gcctttttgg gggccgcaac ccaagcgatc tgcgatccgg aaacatgcgc gcaaaatgca
     1921 gaccagtcgc gccggaagag accaacgggc tttagccggc ctttccaacc tcccggcacg
     1981 gcggttagtc ggcgcgcgcc ccggtgccgc ccaagaacct cgcgcgaccg gactgtgacc
     2041 gcgcctttgc cggagtcggc cgcgcttgca gggctgacgc cggcgccgat tgcacttgtc
     2101 cacacggccg catccgggga caatgctgcg gtttgctgcc tatcgaccgc acaacaggag
     2161 ccaaccatgg accatgccgc gatcgcagac ttcctgaaaa agaacgtcgg ccttttcaag
     2221 cactttccgg atcaccgggt ggcggagctg gttgccggtt cgcaagtggc gtcttacgag
     2281 cccaacgagg ccattgtgga attcggcgag gatgcgaccc atctcggagc cattctggaa
     2341 ggcgaggcgt cggcctcggt catcgctgaa agcggcgacc ggcaggtgct caggcgcctc
     2401 aagaccggcg acaccttcgg cgagatggcg ctcatgaccg gcgagaagac tatgatcgac
     2461 atcatcgcgg agacgcgatg cagcgtgctc ctggtgccgg tcagtctttt ttcgtcggtc
     2521 atcatgaccg agtcgcgggc ggtgcagcac atctcgagga ccatgtcgga gcggttcagg
     2581 acggtcatgc tggacccgtc ccaagcagcc gccttgagaa accaaaaaaa acccattacc
     2641 atacaactga agggtgaacg gccggagaag atcctcgtcc tcaactgcgg ttcgtcgtcc
     2701 cttaagtatt gtctttttga cacgcagaac gagtcgaaca acgttcgagg cctggtcgac
     2761 cgcattggca ccgacatcac ccggcacacc tgccgcggac cgcgcggaga ggttgctcgc
     2821 aatctgccca agggcaccca ccgcgaggcc ttcgaggcca tgttcgccga actgaccgcc
     2881 aaagagaccg gggccgtctc cagcgccgcc gaaatcagcg ccgtcggcca ccgggtggtc
     2941 cacggcggcg agaggttcgc cgagaccacg gtcatcaccg acgacgtgct tcgcgagctc
     3001 gagacgctgt gcccgttggc gcccctgcac aatccgatca acctcctcgg tattcgagag
     3061 gcgctgcgcg tgttcccctc ggtcccgcac tttgcggtat tcgataccgc gttccataac
     3121 accctgcctt cgttcgccta tctttacggc ttgccgtacg agtactacga gaagaagaag
     3181 gtgcggcgtt atggttttca cggtacgtca cacgcctatg tctgtcttgc cgccgcccag
     3241 tatctgtgcc gacgggtcaa tgagctggag atcatcagtt gccatctggg caacggggcc
     3301 tctgtttgtg ccgtggacca cggccggtcc gtcgacacct cgatggggtt gaccccggca
     3361 gagggcctga tcatggggac gcgcggcggg gatatcgatc ctggggttct tgttcacctt
     3421 caacgagaag agggattgac cgccgaccag atcgacgaaa tcctgaaccg taagagcggg
     3481 ctgttgggtt tgtccggcat ttcttccgac atgcgcgagg tcgagcgagc ggctcatgag
     3541 gggaaccccc gggccttggt ggcgctcaag acgttctgct atcggttgcg aaagtacatt
     3601 ggcgcctacg tggccgccat gggcggattg gacgtgatca ttttcaccgc cgggatcggg
     3661 cagggcagcg cccgggttcg cgcgttggcc ctgcagggcc tgcgctgcat gggggtcgtt
     3721 ctggacgagc agcgcaatcg cgaagcccat gcgtgcgagg agatccgccg catttcagcc
     3781 gacgattcgc cggttacggt gctcgtcgtg cccaccgacg aggaacggat gatcgctcga
     3841 gagtgcttgc gagcactcag ccgctcgtac atcaagcaag tccttgaaac gcaacgacag
     3901 gagcctattc ccatcgaggt gtcggcccat catgttcatc tcacacagga acacgtcgag
     3961 gcgctgttcg gcgtcggtca tgagctgacc cggcagagcg atctgtcgca acctggccag
     4021 tatgcctgcc aggagcaggt gacgctgatc ggcccgcgcg gccgcgtcga gcgggtgcgc
     4081 gttcttgggc cggtgcgcca attcactcag gtcgagatcg cgatgaccga gcagttcaag
     4141 cttggcattc atccgcccat ccgcgagtcc ggcgatctga aggacacgcc cggcattacc
     4201 attgaaggtc ccgcagggag cgtcaccatc gacaagggtg tgatctgtgc catgcgccat
     4261 atccacatga cgcccgagga cgcgctccgc tacggcgtcc gcgacaagtt tacagtgcgc
     4321 gtatgcgtac ccggcgacag ggagctggtc ttcggcgacg tgcgcgtccg cgtcgatccg
     4381 agcttccgcc tggcgatgca catcgacacg gatgagggca acgccgcgaa tatcagcacg
     4441 ggcatgacgg gttacattga cggcattcag aatcaggggt gaggtccttg aggggcggcg
     4501 gccgggacgc taatccacgc cggctgccga ttggcaatta tctccgtcct ttcctggtct
     4561 gggggcgctg ctccgaagag tttggcaggt gctgtttgcc gcctcccatt tgatcctgta
     4621 gccgcgccct tcggtcgagc gcgtgggcct gcattgagcg tgcctcgcgc gaagttccgc
     4681 agccgattgc gggcgtcgta gccgtacagt tcgcttcggt ccgtggccat caggtcccga
     4741 gtatacagcc gattgcccac ggcgtcatag ccgtagtcgt atttcaccag catggtcggc
     4801 ggttcgccgt tcttgaaccg ttccatgtgg gtcatccgcc cgttgacgtc gtagtcgaag
     4861 acgctcgaca ccccgttggc aaagctgctg ctgatcacct ggtcggccag gtccaacccg
     4921 cgggtcacgc ctacgcccgt cccgcccgag acgctggtca tccggaagcg tccatcgaac
     4981 gtgtagacga cctcccggct ggctgggtag gtcaccgtgg ccgtgctgtc gggcgtgccg
     5041 atggcatagc tgacggtcgt ggtgtacgtc tcgctcgcgc cgtaggcctg ggtcagactg
     5101 gtcacccggc ccaggatgtc gtactcgaag gtctcgttga ccaggctgtt gttggccgtc
     5161 agcagacgac ctgagcggtc gtacgtgaag gtatcagagc ggcttgggta ggtccgctgg
     5221 agcagccggt acatgttgtc ccactcgaac gtggtcacaa cgcccttctg gtcggttcgc
     5281 g
//
LOCUS       DAOTPW010000325         5255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_13126_length_5255_cov_5.978654, whole genome shotgun sequence.
ACCESSION   DAOTPW010000325 DAOTPW010000000
VERSION     DAOTPW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13126_length_5255_cov_5.978654"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(478..2241)
                     /locus_tag="P5316_20040"
     CDS             complement(478..2241)
                     /locus_tag="P5316_20040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRR87307.1"
                     /translation="MRVPSLIVATVISAVTALPIGLQAAESRPNVVLIMTDDQGYGDF
                     GCTGNPVIRTPHIDSLYADSARMTDFYVSPVCTPTRACLLTGRYNYRTRALDTYIGRA
                     MMEPQEVTIAEVLRDAGYATGIFGKWHLGDCYPMRAMDQGFEESLVHRGGGIGQPSDP
                     PGGEGKYTDPILFHNGHPEPQKGYCTDVYFDAALKWMKACVERKRPFFAYIPTNAPHD
                     PLHDIPQQIYERYKAIDLSPVVLTRDGGKPTKEELDRVARVFAMITNIDENVGKLVAG
                     LDRLGVAKDTMVIFLTDNGPASRRYVGPFRGTKADVHDGGVRTLLLVRWPGRLKAGHT
                     SDRIAAHIDILPTLLEACGLPVPNGLKLDGRSILPLLEGRQVDWPDRTLFIQTHRGDV
                     PILYHHFMARNQDWKLVNASGFAHEEWAGPPQFELYRIVEDPREEKNLAAERPEVVEQ
                     LKKEYEAWFRDVSSTRPDNYAPPRIHIGTTHENPVTLTRQDWRRVDSDKRGYGVRGKW
                     LLHVAKAGSYRMLMRLQPEPAAGQVRLTIGGYEKTAPVTADAKEIVFEGVSLSEGDVE
                     LRADVTRGDKAVGPWQVDVSW"
     gene            complement(2379..2885)
                     /locus_tag="P5316_20045"
     CDS             complement(2379..2885)
                     /locus_tag="P5316_20045"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR87308.1"
                     /translation="MSDTDNTTKASSSRVGERKWLFWAVMLVALASVFIYSNRPVPPS
                     AVEWVADFEAAKAKAAESNKLLLMDFYATWCGPCKAMDRQVFPRSEVAQALGNWVAVK
                     IDVDRQPQIARDFSVDAMPTLVVLTPAGESIARASGYMDASDFIDWIVRAEKIWAEKA
                     RPAAPAGS"
     gene            complement(3056..4927)
                     /locus_tag="P5316_20050"
     CDS             complement(3056..4927)
                     /locus_tag="P5316_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR87309.1"
                     /translation="MSDSRPAVRNPWAWVPSLYFAQGIPYVVAMSVSVIMYKRMGISN
                     TDIALYTSWLYLPWVIKPLWSPLVDLFRTKRLWIVTTQFLIGALLASVALTIPLPKFF
                     QYTIAILWLMAFSSATHDIAADGFYMLGLEQHQQAAFVGVRSTFYRIAMISGQGVLVV
                     LAGLIEGASGLPPRELQVVSKPDAPPVAVALPGTLDVVSSDGPLEIVAEPAILEIGTV
                     AQSKQEIDSILAKVKRTNLSNGFYPAEQSPGEKSSRQLSRPTGGSVERLEEFLRAHFG
                     PKEKASIDRAGNIGVVTLHLSRPPEPGRKVAVVLDRASGDASIRLVEGMRLVFDDGNW
                     NKPALAVFQIDPTLKTEEQAVFRATSGNVRLAWSVTFFVLAAIFITAFVYHYFILPYP
                     AADAPRSGGTSGRFLTEFFRVFAAFFAKKRIVVAVAFLLLYRLAEAQLVKLISPFLLD
                     AREAGGLGLTTARVGIAYGTFGVAALMGGGLLGGYVISRQGLKFWLWPMIIIINLGGW
                     VYLFLAYVQPQSFPLICAAVALEQFGYGFGFTAYMLYMILIAEGEHKTAHYALCTGFM
                     ALGMMLPGMFSGWLQELIGYQHFFIWVLLTTIPGMVVAAMLKIDPEFGRKKAEAAKR"
BASE COUNT         1068 a         1681 c         1566 g          940 t
ORIGIN      
        1 agattcctat ctgggacccc tccgttgacg atccccatcg tcattcgtga gagaacgcgg
       61 aagggattcc gctccgagat acggcccgga aaggattccg ggccgagcca gattccggcg
      121 cgagcgagat ttcgctggtc ccagattcct atctgggacc cctccgttga cgatccccat
      181 cgtcattcgt gagagaacgc ggaagggatt ccgctccgag atacggcccg gaaaggattc
      241 cgggccgagc cagattccgg cgcgagcgag atttcgctgg tcccagattc ctatctggga
      301 cccctccgtt gacgatcccc atcgtcattc gtgagagaat gcggaaggga ttccgcctcg
      361 agatgcggcc cggaaaggat tccgggccga gcgagattcc ggcgcgagcg agattccggg
      421 gcgagcgggc tcgatcatca ctcccccatc tcgaagacga gatggggcac ccgaaagcta
      481 ccagctcacg tcgacctgcc aagggccaac ggccttgtcg ccgcgggtaa cgtctgcccg
      541 gagctctaca tcgccttccg acagcgacac gccctcaaag acgatctcct tggcatcggc
      601 cgtgacgggc gccgtctttt cgtacccgcc gatcgtcagt ctgacctgtc cggccgccgg
      661 ttccggttgc agacgcatca gcattcgata tgagcccgct ttcgccacgt gcagcagcca
      721 cttacccctg acgccgtagc ccctcttgtc ggagtcaacc cgccgccagt cctggcgcgt
      781 cagcgtgacc gggttctcat gtgtggttcc gatatggatc cgcggcggag cgtaattgtc
      841 cgggcgcgtc gaactgacat ccctgaacca ggcttcgtac tccttcttca actgctcgac
      901 gacctcagga cgttcggcgg cgagattctt ctcctcgcgg gggtcttcca caatgcgata
      961 caactcgaac tgcggcgggc cggcccattc ctcgtgtgca aaacccgacg cattgaccag
     1021 cttccaatcc tggttgcggg ccatgaagtg atgataaaga atcggcacgt cgccccgatg
     1081 cgtctggatg aagagcgtgc ggtccggcca atcgacctgc cggccctcca gcagcggcag
     1141 gatactccga ccatcgagct tgagtccatt cggaaccggc aagccacagg cctccagcag
     1201 cgttggcagg atgtcgatgt gagcggcaat gcggtccgag gtgtgaccgg ccttcaatct
     1261 ccccggccag cggaccagca gcagcgtgcg gacaccgcca tcatgtacat ctgccttggt
     1321 accccgaaac ggaccgacat accgccggct ggccgggccg ttgtccgtca ggaagatgac
     1381 catggtgtct ttcgccacgc ccagtcggtc gagacccgcc accagcttgc cgacgttctc
     1441 gtcgatgttg gtgatcatgg caaaaacgcg ggcaacccga tcgagttcct ccttcgtggg
     1501 cttgccgccg tccctggtca gcaccacagg tgacagatcg atggccttgt aacgctcgta
     1561 gatctgctgc gggatgtcgt gcagcgggtc gtggggagca ttcgtgggga tgtaagcgaa
     1621 gaacgggcgt ttgcgctcca cgcaagcctt catccacttc aaggccgcgt cgaagtagac
     1681 gtcggtgcag tagcccttct gcggctcggg atgaccgttg tgaaacaaga tggggtcggt
     1741 gtacttgccc tcgccaccgg gcgggtcgga tggctgtccg atgcccccgc cgcgatgaac
     1801 cagcgattcc tcaaagccct gatccatcgc ccgcatcgga taacagtcgc ccaagtgcca
     1861 ctttccgaaa atcccggtgg cgtaacccgc gtcgcgaaga acctcggcga tcgtcacctc
     1921 ctgaggttcc atcatggccc ggccgatata ggtgtcgagc gcccgcgtgc gatagttgta
     1981 ccggccggtc agcagacatg cccgcgtcgg cgtgcacacc ggactgacgt agaagtcggt
     2041 catccgagcg ctgtcggcat agagcgagtc gatatgaggc gtgcgtatca ccgggttgcc
     2101 ggtgcagccg aagtctccgt agccttgatc gtccgtcatg atcaaaacca cgttggggcg
     2161 gctctcggcc gcttgcaggc cgatcggcag agcggtgacc gctgatatga cagtcgcgac
     2221 aatgagagac gggacgcgca tgatgaatcc tcctgaaagc cgcgcgtaac gataccgcag
     2281 cgtcggccgc aagccaaacg ctctggccag gcctcgatgg cctcatcacc attcagtttt
     2341 cgtaacgctg tgccgccggc gcgggcactg ttgagaggtc acgagccggc tggtgccgct
     2401 gggcgcgctt tttcggccca aatcttttcg gcccgaacga tccaatcaat aaagtccgag
     2461 gcatccatat acccggaagc gcgggcaatg gactccccag cgggggtaag aacgaccagc
     2521 gtaggcatcg catccacgct gaagtcacgg gcgatttgtg gctgtctgtc aacgtcgatt
     2581 ttgaccgcca cccagttgcc gagggcttga gcaacttcgc ttcggggaaa aacctgacgg
     2641 tccatcgcct tgcaggggcc gcaccacgtg gcgtagaaat ccatcagcag cagcttgttc
     2701 gattcggccg ccttggcctt ggcggcctcg aagtccgcaa cccactcgac agccgacggc
     2761 gggacgggtc gattactgta aatgaaaacg gaggccagcg ccaccagcat caccgcccag
     2821 aagagccatt tccgctcgcc gacccgcgaa gacgaggcct tggttgtgtt gtctgtgtcg
     2881 ctcatgcatc cattgtagcc ccgagccggc ggaaaacaca atccggcaac cacgcgaggc
     2941 gactaaggaa ttgactgccc ctgcggaact tcacgtaaca tggttgccgc cttatcccat
     3001 cagtgaaccg ccgggctatt gccaatcgtg ccttcgacgc ggcgatgcga cgaactcatc
     3061 gcttggcggc ttccgccttc ttgcgcccga actccgggtc gatcttgagc atcgccgcga
     3121 ccaccatgcc cgggatcgtg gtcagcagga cccagatgaa gaaatgctga tatccgatca
     3181 gttcctgcag ccagccgctg aacatgcccg gaagcatcat cccgagggcc ataaaacccg
     3241 tacacaacgc gtagtgcgcg gtcttatgct cgccctcggc gatcaaaatc atgtagagca
     3301 tgtaggccgt gaacccgaag ccgtaaccga attgttcgag cgcgacggcc gcgcaaatca
     3361 atgggaagct ttgcggctgc acgtaggcga ggaacagata gacccagcca ccaaggttga
     3421 tgatgatgat catcggccag agccagaact tcaggccctg ccgcgagatc acgtagccgc
     3481 cgagcaggcc cccgcccatc agggccgcga cgccgaacgt tccgtaggcg atgcccactc
     3541 gggccgtggt gaggcccaag ccgccggcct cccgtgcatc cagcaggaaa ggcgagatca
     3601 acttgacaag ctgcgcctcg gccaggcggt acagcaggag gaatgccacg gcaacgacaa
     3661 tccgtttctt ggcgaagaac gccgcgaaga cgcggaagaa ctccgtgagg aaacgaccgg
     3721 acgtgccgcc acttcggggg gcgtcggccg ccggatacgg aaggatgaag taatggtaaa
     3781 cgaacgccgt aatgaaaatg gccgccagga cgaagaacgt gaccgaccac gccaggcgga
     3841 cgttgcccga cgtcgccctg aataccgcct gctcctcggt cttgagcgtc ggatcgatct
     3901 ggaaaaccgc taaggctggc ttgttccagt tgccgtcgtc aaaaaccagc cgcattcctt
     3961 ccacaagcct gatgctggcg tcgcccgacg cccggtccag tacgaccgcg accttccggc
     4021 cgggctccgg cggcctggac agatgcaggg ttaccacgcc gatgttcccg gcacggtcaa
     4081 tgctggcctt ctccttcgga ccgaaatgcg cccgcaagaa ctcctcaagt ctctcgaccg
     4141 acccacctgt cggacgtgag agttgcctgg acgatttttc tcccggtgat tgctcggccg
     4201 gatagaatcc gttgctcagg ttggtccgct tgaccttggc caggatggag tcaatctcct
     4261 gcttgctctg cgccacggtg ccgatctcca agatggccgg ttcggcgacg atctccagcg
     4321 gtccgtctga agaaacgacg tcgagtgtac ccggcaacgc gaccgccacc gggggcgcat
     4381 ccggctttga caccacctgc aactcccgag gcggcaatcc gctggcccct tcgatcagac
     4441 ccgccagaac aaccagcacg ccctggccgg agatcatcgc aattcgatag aatgtgctgc
     4501 gaacgcccac gaacgccgcc tgctggtgct gctccaggcc aagcatgtag aaaccgtcgg
     4561 cggcaatgtc atgagtggcc gagctgaatg ccatgagcca caggatcgca atcgtgtact
     4621 gaaagaattt cggaagcgga atcgtcaagg ccaccgacgc cagcagtgcg ccaatcagaa
     4681 attgcgtcgt cacgatccat agccgcttgg tgcggaacag gtcgaccagc ggactccaca
     4741 gcggcttgat cacccacggc aggtacagcc aactcgtgta cagggcgata tcggtgttcg
     4801 agatccccat ccgcttgtac atgatgaccg acacgctcat cgcgacaacg tagggaatac
     4861 cctgggcgaa gtacaatgag ggaacccacg cccacgggtt cctgacggcg ggtcttgaat
     4921 cgctcatcat tcgtgtcctg aatcagcggg aagtcgcgcg tcccggccgc gaacgccgta
     4981 cggccgacga gaaagactat cgcggatcgc gccgggaagg tcaagaaaac ccgcagtgat
     5041 tgatgttggg catgtccatg acatggtcag ccggcaaggt agggcaggtc agagcgaagc
     5101 gccgacctgc cgctaacgaa atgggtccac gagaacgctg gccgcgctac ccccctgccc
     5161 gatcacggcc tcctcgacgg tgcatgcttc cagcatcgcc gggtccgcgt cgcagcaacg
     5221 tagggcaggt cagagcgaag cgccgacctg ccgct
//
LOCUS       DAOTPW010000326         5178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_13346_length_5178_cov_6.436853, whole genome shotgun sequence.
ACCESSION   DAOTPW010000326 DAOTPW010000000
VERSION     DAOTPW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13346_length_5178_cov_6.436853"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1141..1455)
                     /locus_tag="P5316_20055"
                     /pseudo
     CDS             complement(1141..1455)
                     /locus_tag="P5316_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461349.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1581..1730
                     /locus_tag="P5316_20060"
     CDS             1581..1730
                     /locus_tag="P5316_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87310.1"
                     /translation="MRLFEKDSDCAAFERVLAEAREREEMRVCAYALMLNHFHLVLWP
                     RRDGG"
     gene            1814..2242
                     /locus_tag="P5316_20065"
     CDS             1814..2242
                     /locus_tag="P5316_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87311.1"
                     /translation="MRTIFLGALHPAVRASVAVCSMAEYQPMTCNHIGNGIGFTKLVP
                     GLYTDLDWPDVAILHWPNALMTINGTKDTLYPLAAARPAVAKIRRIYAQMGAADRYEG
                     VFFEGPHEFNLQMQERAFDWPARQLYLEKDVKPGNHAGGS"
     gene            2232..3005
                     /locus_tag="P5316_20070"
     CDS             2232..3005
                     /locus_tag="P5316_20070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR87312.1"
                     /translation="MDRDLARHFAQRAATYDLAPWVRDPRIMATTIDFIDPRPGQRII
                     DLGAGTGAVLEAILAACPRLGLCVAVDISPEMLARVRDSRIRACRSDVQQLPWADASF
                     DVAVCRQTLHYVEDLHRCLAEIRRVLRPEGTLVIGQMTPFGDEDEIYWETIVRLRQPL
                     RRHDLTAAELIGLVRSKGFQVIRTTQQRARESLNSWLARYEDTDRQLAELRRLHLDAP
                     ETYRRIHNFERRGDDILLDNCWTFLRATVQPGPAASATR"
     gene            complement(3126..3234)
                     /gene="rrf"
                     /locus_tag="P5316_20075"
     rRNA            complement(3126..3234)
                     /gene="rrf"
                     /locus_tag="P5316_20075"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            3719..4033
                     /gene="rplU"
                     /locus_tag="P5316_20080"
     CDS             3719..4033
                     /gene="rplU"
                     /locus_tag="P5316_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRR87313.1"
                     /translation="MYAIIEDGGRQYKVSQGDKIYVDIRELPEGQETIEFTSVLALGD
                     GENARIGRPFVEGAKVVAKINGQVKAPKVTTIHFRRRKNYRKKIGHRQQHLAVTISDI
                     VG"
     gene            complement(4153..>5178)
                     /locus_tag="P5316_20085"
     CDS             complement(4153..>5178)
                     /locus_tag="P5316_20085"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HRR87314.1"
                     /translation="RRSAADRAKLPPEFACFGISEVMPSAGHAILRTAGPAGLTAAFT
                     FSPYGGSHGHFDKLSFVLFGWNRELGVDPGRAASQAYRLPIHKNWYKATISHNSVVVD
                     GQSQQPVAGSLRLFASNERYAAVVADCDTAYKGIVHRRMLVMTPTYLLVWDRLDSPDE
                     DRRFDWFYHNRGHEVQCEAAGEQADLSAEPPGYQYIQQAIQGTSTGPVAIAFLDDTVT
                     THLTVAGNGQVQIVVGDGPGSSILERVPLAMIRQTGRSVRFAAVIEPVTGSAKPSVSS
                     VEWREESGGVTIEIMSGPAVESVRLAGDLGIEIRSGQKTVLTSRPAGEQPAQFPHTGV
                     SGQASNE"
BASE COUNT          978 a         1606 c         1540 g         1054 t
ORIGIN      
        1 tgcggtccaa caccccgaaa aaaaactaga atcgcccttt ggtgttcaac ttgggttagc
       61 gcatgggcgg ctgaagatgt gcagcagatg ctggcgcaac acggcatcgc ctcccgaatc
      121 ctctcaggaa cgcccgttga ttggtccgag tggcacagca gaggacgtgt gcgtttgcag
      181 agactgtcct ccctctcgtg gattctcttc tcgctttggc tgattgctgt tggcataggc
      241 tgcgttttct cgggtcttgc gcgtcgatac tataccgacg gagtcccgcg atgtcggcga
      301 tgtgggtaca tcgtgttcgg actgcgcggc accacgtgtc cggagtgcgg aagcgatctc
      361 gcaggtgatg ggattcgctg gttcgaatcg aggaccgttc ttccacgtgt gctgggcgga
      421 tcgctcttgg ttttgacagg cctatgtctg gcgacgcaat ggtagatacg gaaaaggata
      481 agataacggg ataagaaggg tgtcgaaacg gggatgcagc tggttttccg ggagcagtcg
      541 gcccctcaac agagctcggg gcaagcattc agcattcacc cattagcccc cgtcttgtca
      601 agcgcgaaaa ctcccttggc gaatctttct tggtcagatc gattgcctga aatcgccgaa
      661 gttgccggct ttggtcgttg tacgcgtccc ggttctcgtt tcgcatggtt gtcgtgcatc
      721 tgtcgggcac gcaccagtca cccatcagga tcaaggccgt cgggacggtc cgcgaggaac
      781 ccttcccggc ccctggctct ttcgagcccc agaaaaccgt cggtacccga tcgtgtccaa
      841 ttgactggcc gttatccgca agtggacagc ggcctgacag atacctggct tgcggccgcg
      901 tggaccgctc cactcctttt ctggctcacg atcgaggcgt acccgcctgc aaaaccggcg
      961 ccgcgttcgg cgctgctgtg ccccctcgct ttccggaggg gaaaagccgg agctcccctc
     1021 ttgaacagcg aggcacggcc aatactgaga aggatcggaa gcggcggccc aatcaggatc
     1081 gatggttcga agccaaacac atttccgggc tcgccgctcc gtcctcagtc gattaccgtc
     1141 tcatccgaaa gccctcgtga tgtcgaacgc cacgtcttcc ttgatcacgt ggtagcaggc
     1201 tcgggccagc ttgtgggcca ccgccttgag agccacaatg cgtttcgtgc gggcctgctt
     1261 gcgttggtaa tagcgacgga tcgtctcgtc gtaccgaatc gcgaagttgg ccgcctcgat
     1321 gaacgcccag cacagaaagg cgttgccgtt cttggtattg cctgagccct tgcgttttcc
     1381 gttggtcatg tacgaactgc ccaccaggcg agcgtacgac acgtagttgc ccacatcgcg
     1441 aaaccggctg atatcaggtg gggacaggtt cgttttttga cacgcgagac gagtgatggt
     1501 agactgcggg gatacctcgc agtgctcgtc aagctcccgg cggtatggtg tttcacgtct
     1561 taagccgtgg cgtggggcgg atgcgtctgt tcgagaagga ttcggattgt gcggcgttcg
     1621 agcgagtgct ggccgaggcc cgggagcgtg aggagatgcg ggtgtgcgcg tacgccctga
     1681 tgctcaacca tttccatctg gttttgtggc ctcgacggga cgggggctaa tgggtgagcc
     1741 gttttccgct ctcatgccat tccgctcagc ccccttttcc gctcacggat gtctgggcct
     1801 gagcctcggt tccgtgcgca ccatcttcct gggcgcgctg catcccgctg tccgagcatc
     1861 ggtcgcggtc tgctcgatgg ccgagtatca accgatgacc tgcaaccaca tcggcaacgg
     1921 gatcggcttc accaaactgg tgcccggcct gtacaccgat cttgactggc ccgacgtcgc
     1981 catcctgcat tggcccaacg cactcatgac catcaacggg acaaaggaca cgctgtaccc
     2041 gctcgccgct gcccgcccgg cagtagccaa aattcggcgg atctatgccc aaatgggagc
     2101 cgccgacagg tacgaaggcg tgtttttcga agggccgcac gagttcaatc tccagatgca
     2161 ggaacgagcg ttcgattggc cggcccgcca actttacctg gagaaagacg tcaaacccgg
     2221 gaaccatgcc ggtggatcgt gatctggccc gacatttcgc tcagcgcgcc gcgacctatg
     2281 acctggcgcc gtgggttcgc gatccgcgca tcatggccac gacgatcgat ttcattgatc
     2341 cccgccccgg gcagagaatc atcgaccttg gggcgggaac cggagccgta ctggaggcga
     2401 tactggccgc gtgtccgcgg ctcgggttat gtgtcgccgt cgatatctcg cccgaaatgc
     2461 tggctcgggt ccgggactcc cggattcgag cttgcaggag cgacgttcaa caactgccgt
     2521 gggccgatgc ttccttcgac gtcgcggtct gccggcagac actccattac gtcgaagatc
     2581 tgcatcgttg cttggcggag atccggcgag tgctgcgccc tgagggtacg ctggtcatcg
     2641 ggcagatgac gccctttggc gatgaggacg agatctactg ggagaccatt gtccgtcttc
     2701 gccaaccgtt gcgccgccac gacctcaccg ccgccgaact gataggactt gtccggagca
     2761 aaggcttcca agtaatccgg accacccagc aacgggcccg cgagtcgctc aactcatggt
     2821 tggcccgcta cgaggacacc gatcgtcaac tcgccgaact gcgccgcctg catctcgatg
     2881 cacccgagac ctaccgcaga atccataact tcgagcgacg cggcgatgac attctgctgg
     2941 acaattgttg gacttttctc cgggcaacgg tgcaacccgg acctgcagcc tctgctaccc
     3001 gctgacgtgt tctcccggat ccggagcatg cccgtgaggt tccccggaac tcctgttctt
     3061 gccggatcgg cgcgcgaaaa aacccgctgc ctttcggccg gcgggtttcc gctaaaacac
     3121 acaaatgtcg gcagtcacct actctcgccc cgtgggacta ccatcggccg tctgggcttc
     3181 actgccgtgt tcggaatggg aacgggtgtt tccccaggcg catcagccac cgacaaatca
     3241 tatattaccc atccgaccgc atctgtcaag cccgcccggg gcatcccttc tcagcgggca
     3301 accacaacag ataacccgag gatccctttg ccttaagtgc tttcagcaga acaatttagc
     3361 aggcatgttg aggcctccgc aaagcctcgc tgatcgggta cccgggcacc tgacagattc
     3421 ggctgggcag gcgatacggt tcttccagag ggtactctca gaaccgagac cgtaaggggc
     3481 ggctccgggc gtcgcacccg ctctgtggct tggttccgcc ctcgcgcttg gggtcgcaga
     3541 tagctgccca aagtgtccgg gccacgccgc tcatcctcaa actgccacca gcctccttga
     3601 ccctatccct gccaaagcac ataatgtggg gctcgagaga cgacccgcga aacgacatgg
     3661 cccaggcatg ggacctgtca gtttccactg taactaacct cgtgctggag ttggactggt
     3721 gtacgcgatc atcgaagacg gcggacgaca gtataaggtt tcccagggcg acaagatcta
     3781 cgtggatatt cgggaattgc ccgaaggcca ggagacgatc gagttcacca gtgtccttgc
     3841 gctcggcgac ggggagaacg cccggatcgg ccggcctttc gtcgaagggg ccaaggtcgt
     3901 cgccaagatt aacggccagg tgaaagcccc gaaggttacc accatccatt tccgacgccg
     3961 caagaactac cgcaagaaga tcggccaccg ccagcaacac ttggccgtca ccatcagcga
     4021 cattgtcggc tgatcgttat ccctgcggga aaaccaggcg ggtagcatgt ggggctctcg
     4081 gtgtgctcgg ccggcgagca tcctccactg atcgctgata ccccggtgtg gggaaattgg
     4141 agccatcact cattactcat tgcttgcttg ccctgatacc ccggtgtggg gaaattgggc
     4201 cggttgctca cccgccggcc ggctggtaag cacggtcttc tgaccggagc ggatttcgat
     4261 tcccaagtca cccgccaatc gtaccgactc cacagcgggg cccgacatga tctcaatggt
     4321 taccccaccg ctctcctctc gccattcaac cgatgataca gaaggcttcg cgctgccggt
     4381 gaccggctcg atgaccgctg cgaacctcac cgaacgaccc gtctgacgaa tcatggccag
     4441 cggtacgcgt tccaggatcg acgagccagg gccatcgccg accacaattt gaacctgccc
     4501 attgccggcc acggtcagat gggtggtaac cgtatcatcg agaaatgcga tcgcgaccgg
     4561 cccggtgctc gtgccctgta tggcttgctg gatgtactga taccccggtg gctcggccga
     4621 gaggtcagcc tgttcgccgg ccgcttcgca ctgcacttcg tggccacggt tgtggtaaaa
     4681 ccagtcgaat cggcgatctt cgtcgggcga atcgagccga tcccaaacca acagatacgt
     4741 cggggtcata accagcattc gccgatggac aatgcccttg tatgcggtgt cacagtcggc
     4801 cacgacggcc gcatatcgct cgttgctcgc gaacagccgc agcgagccgg caacgggctg
     4861 ctgggactgg ccgtccacca caacgctgtt gtggctgatc gtggctttgt accagttctt
     4921 gtggatgggc aagcggtatg cctggctggc ggcccggccg gggtcgacgc ccagttcacg
     4981 attccagccg aacaacacga agcttaactt gtcaaagtgc ccgtggcttc cgccgtatgg
     5041 gctgaaggtg aatgcggccg tcaagccggc ggggcccgct gtccgcagaa tcgcgtggcc
     5101 ggcgctgggc atgacttcac taatcccaaa acaagcgaac tccggtggca gttttgcgcg
     5161 atcggcggcc gatcgccg
//
LOCUS       DAOTPW010000327         5091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_13612_length_5091_cov_5.376489, whole genome shotgun sequence.
ACCESSION   DAOTPW010000327 DAOTPW010000000
VERSION     DAOTPW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13612_length_5091_cov_5.376489"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1208)
                     /locus_tag="P5316_20090"
     CDS             complement(264..1208)
                     /locus_tag="P5316_20090"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRR87315.1"
                     /translation="MTAGKVVIIGAGEVGSTCAFALQMRGAAREIVLIDANHERAEGQ
                     AQDLSHGQCFTPPVEVRAGDYPDCSGAQVIIITAGAAQKPGQSRLDLVGTNVEICRSV
                     VGRIIEHTREAIVVMVTNPVDVLTYAAIRFSGLPRGRVIGSGTVLDTARFRFFLGRHC
                     RVDPASIHGYVLGEHGDSEVVAWSITTMAGMRMADYCRACPHKCPQMDRQRIAMQVRN
                     SAYHIIEAKGATNFAVALALLRITTAIIRNENSALTVSTLLQGEYGLTDVCLSVPTIV
                     NQNGADRLICPELSAEDLKALRASAEAIRSVQQNVGLM"
     gene            1398..2678
                     /locus_tag="P5316_20095"
     CDS             1398..2678
                     /locus_tag="P5316_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRR87316.1"
                     /translation="MSSYHCCVVALAVFLSGGGPALAGQGDGPVKLSITPDGRLMLNG
                     RPHFFIGFAPGPPLDFQTPEGGDGWAEMAEGGMSVVRGGPSRDGWTPQAEEQFSQYLD
                     AAHQRGVYVWPFLREMVELNRPGMRQRLEAFIRKYRSHPGILFWKSADEPEWGKLPVE
                     PLKQAYDLIHELDPARLVWFCHAPRGTLETLRPYSAACDVLSIDIYPVSEPPGKHSLD
                     ANKGLSMVGDYTRRTVELAKGGKMPFMVLQVCWSGVNPAHNPKNRLMFPTFRQERYML
                     YQAIICGANSVSFFGMPVGLTGRDAELGWNWTFWRAVLRPLLAEIRPGSELYPVLTLP
                     DSKYPLEFTGAPQIEARWKEASVYLYILAAAREGETVEVTFSGLQDGEVTVLHENRTL
                     QVVDGSFTDTFAPHDVHLYRALRLLSAASRPASR"
     gene            complement(2769..3182)
                     /locus_tag="P5316_20100"
     CDS             complement(2769..3182)
                     /locus_tag="P5316_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87317.1"
                     /translation="MSRGHAEQSEPSGKRKTIVLIVALAGLAIAAVAVAWRNREEKQP
                     DTPESAMTYICTECKHVYALTPAGYEKLTKEGGIKAPDTREDGGVVLFRCPSCGKFAA
                     VPAVECPNDGTQLPRRLPNGRPGRCPKCNWSFYPR"
     gene            complement(3301..4203)
                     /locus_tag="P5316_20105"
     CDS             complement(3301..4203)
                     /locus_tag="P5316_20105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR87318.1"
                     /translation="MQCKKRLHAFTLIEILVVVAIIALLIAILLPSLQRAREQAKIAM
                     CLANLRSLGVAATSYLTAEKDQFCWAKAGRYPASNFYGGKRGNGDGEESYLSGYYWGG
                     SLNFETRFRPLNRYVYGRVVNDVTELRVFECPSDYGVRSRYAALQPPSKKNAYEVTGT
                     SYQANVIWYTYLLDVENLRGNADELSRRVGSLEKSIMRHFRRFGASRAILLQEDPCDV
                     STGGVLTGSWPRDVKMLGWHRQFDRHNLLFLDGHAAYTYVNAKMNLDHRAGSTMFVEC
                     SPSQLKNCVHGTSDWISHLDYGAE"
     gene            4710..>5091
                     /locus_tag="P5316_20110"
     CDS             4710..>5091
                     /locus_tag="P5316_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87319.1"
                     /translation="MQSKSLVVLSMVAVLGLVSAAMAVPYASGVVQNGASSTFVLNED
                     GANVKIVYDGGATVVNMGTLNKGTYTFDATLGSSYQIKVSKSAAVGWTQISDDNLTQS
                     KYYSPRGVSVNKNAASSNFGRIYVS"
BASE COUNT          959 a         1600 c         1502 g         1030 t
ORIGIN      
        1 gatgctccgg ctgggccgca tgacggaggc acgaaccctg ctggaagaga tacttcacga
       61 gaacgctgac aaccttctgg ccggcagact ctgggatcaa aaccggcccg gcggggctcg
      121 gcggtaaagc gaccgacgat agagcagcgt cgcaatttcc tgcggcaccg agacctgcgg
      181 ctgtcccgtc tttgccgcag cggatcgtcg tattcgcact ctgtcattcg tgtcgactcg
      241 agtgtcccgg ttattctcgt tcgtcacatg agtcccacat tctgctgtac gctgcgtatc
      301 gcctccgccg acgccctgag ggccttcaaa tcctccgccg acagttccgg gcagatgagg
      361 cgatccgcgc cattttgatt gacaatcgtc ggcacgctca ggcaaacgtc ggtcagaccg
      421 tattcgccct gcagaagagt ggaaaccgtc aaggcgctgt tctcgttgcg gataatcgcg
      481 gtggtgatcc gcagcaatgc cagcgccacg gcgaagttgg tcgctccctt cgcctcgatg
      541 atgtgatagg ccgagttgcg gacctgcatg gcgatccgct gacggtccat ctgcggacac
      601 ttgtgcgggc aggcgcggca gtagtccgcc atgcgcatgc ctgccatcgt cgtgatgctc
      661 caggcgacca cttcgctgtc gccgtgctcg ccgagtacat aaccgtggat gctcgcagga
      721 tcaacgcggc agtgacggcc gagaaagaaa cggaatcgcg cggtatccag tacggtgccc
      781 gagccgatga cacggccgcg aggcaaaccc gagaaccgga tcgccgcgta agtcagcacg
      841 tccaccgggt tggtcaccat gaccacgatc gcttcgcgag tgtgttcgat gattcgacca
      901 accaccgacc ggcatatctc cacattggtt ccgaccaggt ccagccgcga ttgccccggt
      961 ttctgtgccg ctcctgcggt aatgatgatg acctgggcac cgctgcaatc gggatagtct
     1021 cccgctcgga cttccacggg gggcgtgaag cactgtccgt ggctcaggtc ctgtgcctgc
     1081 ccctccgcgc gttcgtggtt cgcgtcaatc agaacgatct cgcgggccgc gcctcgcatc
     1141 tgtagggcga aggcgcaggt gctccccacc tcaccggccc cgatgatgac gaccttcccg
     1201 gcggtcatgc tcatgttggc tcctcggtgc ccgtccggct aggcttatcc gtggttgctt
     1261 tcctcccatc ccttgcctgg cccgcgcaca agttcggggg ccggtcctgg atggggtccc
     1321 ggatgcatac aaagacgcac agggcacgca aaacatcata ggccctgcgc ttgcgggaag
     1381 acagacagga gacatgaatg agctcttacc actgctgcgt cgttgccctt gcagtgtttt
     1441 tgtctggtgg aggtccggcc ttggccggac agggcgatgg ccccgtgaag ctgtcgatca
     1501 cccccgacgg ccggctgatg ctgaatggcc gcccgcattt cttcatcggt ttcgccccgg
     1561 ggccgcctct tgatttccag acgcccgagg gcggcgacgg ctgggctgag atggccgagg
     1621 gtggtatgtc cgtggtccgc ggtggaccat cccgcgacgg atggacgcct caggccgagg
     1681 aacagttttc tcaatacctt gacgcggctc accaacgcgg tgtgtacgtc tggccgtttc
     1741 tgcgtgaaat ggtcgagctg aacaggccgg gtatgcgaca aaggctcgag gcctttatcc
     1801 gcaagtaccg gagccacccg ggcatcctct tttggaagag cgccgacgag cccgaatggg
     1861 gcaagctccc agtcgagccg ctcaagcagg cctacgacct cattcatgag ctggatcccg
     1921 cccgtcttgt gtggttctgc cacgcccccc gcggcacgct ggagacgctg cgtccgtaca
     1981 gcgccgcctg tgacgttctg agcatcgaca tctacccggt cagcgagccg cccggcaagc
     2041 attctctcga tgccaacaag ggcctaagca tggtgggcga ttacacccgt cgaacggtgg
     2101 agctggccaa aggcggtaag atgcctttca tggtcttgca ggtttgctgg agcggcgtga
     2161 accctgctca caaccccaag aaccggctga tgttccccac gtttcgacag gaacgctaca
     2221 tgctctacca ggcaatcatc tgcggcgcca acagcgtgtc gttcttcggc atgcccgtcg
     2281 gactcaccgg tcgtgacgcc gaactcggtt ggaactggac cttctggcgt gcggtgctca
     2341 ggccgcttct ggccgagatc aggccgggaa gcgaactgta tcccgtgctg actctgccgg
     2401 actcgaagta tccgttggag ttcaccggcg cccctcagat cgaggcccgg tggaaggaag
     2461 cgagcgtcta cctgtatatc ctcgcggccg cccgcgaggg cgagacggtc gaggtgacgt
     2521 tttcggggct gcaagatggt gaggtcacag tcctgcacga gaatcgcacg cttcaggttg
     2581 ttgacggctc gttcacagac acctttgccc cgcacgatgt acacctttac cgggccttgc
     2641 ggctgctgtc agcagcgtcg cgccccgcgt ctcgctgaga taccgcggca ggaacaaaag
     2701 tccccgccgc gaaccgcgag ttcacccctg gagcaggaat cgtccagatg tctctacgaa
     2761 agcgcaggct atcgaggata aaacgaccag ttgcacttcg ggcagcggcc cggtcgaccg
     2821 ttcggtagtc gccgcggcag ctgggttcca tcgtttgggc attccacggc cggaaccgcc
     2881 gcaaacttgc cgcacgaggg acagcgaaac aagactaccc cgccgtcttc ccgagtgtcg
     2941 ggagctttga ttccgccttc cttggtgagc ttctcgtaac cggccggcgt caaggcgtag
     3001 acgtgtttgc actcggtgca gatgtaggtc atcgccgatt cgggcgtgtc cggctgtttc
     3061 tcctcccggt tcctccaggc aacggcaact gccgcaatcg ccagacctgc cagcgctacg
     3121 atcaggacaa tcgttttccg cttgccggaa ggctctgact gctccgcgtg gccacgactc
     3181 atcacaaact ccccgatgcc cagacttcgt tatgaggggc tctgctaccc gtgccgccgc
     3241 ccctcagcca gggccgtcac gccgaaggca tccggcggac acccaccggc ggcgcaaacc
     3301 ctactcggcg ccgtaatcga ggtgcgagat ccagtcgctt gtgccgtgaa cacagttctt
     3361 caactggctc ggcgaacact ccacgaacat cgtcgagccc gcacggtgat cgaggttcat
     3421 cttggcgttg acatacgtgt aggccgcatg accgtccagg aaaagcaggt tgtggcgatc
     3481 aaactgccgg tgccaaccga gcatcttgac gtcgcggggc cagctacccg tcagaacgcc
     3541 gcccgtcgat acatcacaag ggtcttcctg aagcaggatg gcccggctcg cgccaaatct
     3601 ccggaagtgc ctcatgatgc ttttttccag cgacccgacc cgacggctca gctcatccgc
     3661 gttacctctc aggttttcaa catccaggag gtaggtgtac cagatcacat ttgcctgata
     3721 actcgtgccc gtgacctcgt aagcgttttt cttggagggc ggttgcaggg cggcatagcg
     3781 gcttcgcact ccgtaatctg aagggcattc gaacacgcgc agctcggtca cgtcgttcac
     3841 cacccttccg tatacgtacc ggttcagcgg gcggaacctt gtctcgaaat tgaggctccc
     3901 gccccaatag tagcctgaaa gataactctc ctctccatct ccgtttcccc gcttgcctcc
     3961 gtagaaattc gaggccggat atcggcccgc cttcgcccag cagaactggt ctttttcggc
     4021 ggtcagatat gaagtcgccg caacaccgag cgaccgcaga ttggccaggc acatggcgat
     4081 cttggcctgc tcccgcgctc tttgcagcga cggcagcagg atggcgatca gcagggcgat
     4141 aatggccacg acaaccagga tttcgatcag ggtaaacgca tgaagccgct ttttgcactg
     4201 catgaggtga gcccttccag ttacactcga ggcgtttctc gacctgaatg gtcctttcta
     4261 taccgccgac ggccatgccc gtgaccggcc gcactggact ggttatcaac ccctttagtt
     4321 tatcgcaatc agcccgctgg ggcaacgcaa aggcccgatc gttcaatcgt tcatcaaaga
     4381 cagcccttgc tgcagaagaa tcagtgatac tgaagcaccg ccgaataccg ccttgccagg
     4441 gcccggccgg cgacggcttt cctcaagatt ttccttgcca tgcgccggtt tccctggtag
     4501 cctagtcagc gtggggtata ccggaatcgg ggagaggaaa agcaccactg ttccgctctt
     4561 ccctgaaggt tccccgggtg cagaaacatt tcggttgttg agagggttgc ctttctggcc
     4621 gaaaccgggc gagccgctgg aacgacagct cagcggtcga acggttgtcc agtggagcct
     4681 gtcacagggt cttttggagg agatcgatca tgcaaagcaa atctttggtt gttctgtcta
     4741 tggtcgccgt tctgggattg gtttccgcgg cgatggccgt cccctacgcc tcgggcgtcg
     4801 ttcagaacgg ggcaagctcg acttttgtcc tgaatgagga cggcgccaac gtcaagattg
     4861 tctacgacgg cggcgccaca gttgtgaaca tgggaacgct caacaagggc acctatacat
     4921 tcgatgccac gctgggatca agctaccaga tcaaggtcag caagtccgcc gctgtcggct
     4981 ggacgcagat cagcgacgac aacttgaccc agagcaagta ttactcgccc aggggtgtct
     5041 ctgttaacaa gaacgcggcc agctcgaatt tcggaagaat ctatgtcagc g
//
LOCUS       DAOTPW010000328         5042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_13778_length_5042_cov_5.268097, whole genome shotgun sequence.
ACCESSION   DAOTPW010000328 DAOTPW010000000
VERSION     DAOTPW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13778_length_5042_cov_5.268097"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="P5316_20115"
     CDS             <1..524
                     /locus_tag="P5316_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87320.1"
                     /translation="LQCRAGDISHRELLLRPTPDAYREMAADWIEIHLKGVVPGGVIE
                     RAMSAIRQSGNRRTRLVEAQILADADDLDQIGPPYIDLMIRKCRWEGKTMEQLLTAWQ
                     RQEEYKYWEARINESLRFEAVRAIARRRHEALRQFMASLTATIKLDDLAALAKSHREQ
                     SPPLGAVAPAEPQ"
     gene            826..2100
                     /gene="murA"
                     /locus_tag="P5316_20120"
     CDS             826..2100
                     /gene="murA"
                     /locus_tag="P5316_20120"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRR87321.1"
                     /translation="MDYFRINGGKRLRGTVDINGAKNAALPIMAASILCEGEVVLRNV
                     PDLMDIRSMEKLLNKLGVSSVREPGGTLRLKVQDEMNCHADYELVRQMRASVCVMGPL
                     LGKRGRSQVSLPGGCAIGDRPINLHILGLQALGAEADLINGDVLLKAKRLTGTELFLG
                     GPFGSTVTGTANVMMAAVLAEGRTVIESAACEPEVADLANFLNACGARITGHGSPRIT
                     IDGVKKLTGCEYTIIPDRIEAGTFIIAGAITNGEIRLRNCRLEHLLALVDRLRLIGVN
                     VEREGDEVVVSSTRRLEPVDITTQPYPGFPTDLQAQTMALLCLADGNSIITEKIYPDR
                     FMHVGELSRLGAKLRKEGPTVIIQGVKRLVGAPVMASDLRASAALVLAGLVAKGETRV
                     NRVYHIDRGYERVERRLQALGADIERRSGEEE"
     gene            2674..3675
                     /locus_tag="P5316_20125"
     CDS             2674..3675
                     /locus_tag="P5316_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87322.1"
                     /translation="MLSPDYDPGNRSICRNVLVCLALVATLGVAGCHCGGGLCAGAGG
                     SQTQLHFYAPPGAMVEIQALGPNSSRQILPPGAFGDRLERTPEEACVFNLSPGCYEFK
                     YVGVEGVPGAAIYGELDVRHANSSMGRIFQRRSAIPIALPSEYYRRVEARGDELVPYR
                     GEGYRTAIDEQDMIRVKAGDVVEKVFVVADLEKADKLLRKTEVEIAAAEREIEYAEAR
                     FREAYYNFRTDVSDASANFWGTDREFIRWERKRQRAQQNLDKLIALRQRTQSLIKADR
                     VMIREGMLVLATQEVVRPYRDVVEAAEEIGEVLLVMRIGGRHMQWGEPPGELAAYNP"
     gene            3747..4820
                     /locus_tag="P5316_20130"
     CDS             3747..4820
                     /locus_tag="P5316_20130"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRR87323.1"
                     /translation="MSDNRFRFAGAVLAVVLLLAGGVGCCLDKQARITVASATDVTAG
                     TADRGAPLPAGVSLPPGFYPLSGQYDLENPDDHYGGWPRYIMCVADGMVMAYVPAQEF
                     MMGGGTDLDEVPARAVRVNHFYVDIHEVTNCQFGRYSRQGPYRQFYMPGVNDHHPVRN
                     VSWVEAHGYAAWAGKSLPTEAQWEAAARGSDRRIYPWGNEEQSDVTRYLCNASTGRDY
                     YDGYQGPAPVMSYSAGISPFGAFNMAGNVWEWCADWYDPGRYAYPSEEDPPSALERGA
                     LPFGDRNYPSPKHKDIREARVGPPVGLERVIRGGSFMDPIQQCRVDTRASLPPAARRN
                     NVGFRCVLMLPPTEDNDLALASR"
     gene            4863..>5042
                     /locus_tag="P5316_20135"
     CDS             4863..>5042
                     /locus_tag="P5316_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87324.1"
                     /translation="MTDAGRLLAGLLSFSAEEGLGTVLGSGCYVLRTLSAKDVDGVGR
                     LGEVFGVERMTGNWPR"
BASE COUNT          950 a         1375 c         1682 g         1035 t
ORIGIN      
        1 tattgcagtg tcgggccggg gacatttcac atcgtgagct gctacttcgt ccaacaccgg
       61 atgcctatcg tgagatggct gccgactgga tagagattca tctcaagggt gtcgtgcccg
      121 gaggggttat tgagcgggcc atgtctgcca ttcggcagtc gggcaaccgc cggactcgtc
      181 tggtggaagc ccagattctg gcagatgcgg acgatctcga ccagatcggg ccgccgtaca
      241 tcgacctgat gattcgcaag tgccgctggg agggcaagac catggagcaa ctcctgacgg
      301 catggcaacg gcaagaggaa tacaaatact gggaggctcg tatcaacgag tcccttcgtt
      361 tcgaggctgt tcgtgccatc gcccggcgac gccatgaggc cttgcggcaa ttcatggcca
      421 gcttgactgc caccatcaag ctggatgact tggcggcgct cgccaaatcg catcgtgagc
      481 aatcgccacc gcttggtgcg gtagcgcccg ctgagccgca gtaggtaccg gtccctattc
      541 cctatctgct cgtagccgac cagcgagcgt cgataatccg tccaagggcg tccaaatcgg
      601 cttggaccgg gccagcggta tcggctccat ccgtgctcag cgggttcgac aagacgacac
      661 gaagacttgt gaaataggcg atttaggccg atgatgaata ccgacgaaag ccagtgctgg
      721 cgtgagcaca gggctttcat tggcctccaa ggctggggtg gtgtatcagc ctaaggcagg
      781 tgtgcgttgg atcaagggta gttaccgatc tgaaggagcg ggtctgtgga ctattttcgc
      841 atcaatggcg gcaagcgatt acgtggtacg gtggacatca acggggcgaa aaacgccgca
      901 cttcccatca tggcggcatc aatcctgtgt gaaggcgagg tcgtcctcag gaacgtccct
      961 gatctgatgg acatccgttc gatggaaaag ctcctgaaca agcttggggt ttcctcggtg
     1021 cgagagccgg gaggaaccct tcgactaaag gttcaggatg agatgaattg tcacgcagat
     1081 tatgaactgg ttcgacagat gcgggccagc gtttgtgtga tggggccgct tctcggcaag
     1141 cggggtcggt cacaagtcag ccttcccgga ggatgtgcca tcggcgaccg gccgatcaat
     1201 cttcacatcc tgggcttgca ggcattgggc gcagaggccg acttgattaa cggcgacgtt
     1261 ctcctgaaag ccaagcggtt gaccggtacg gagctattct tgggagggcc tttcggctcc
     1321 acggtcaccg gtacggccaa cgtcatgatg gcggccgtac ttgccgaagg tcggacggtg
     1381 attgagtccg cggcgtgcga gcctgaagtg gccgacctag ccaactttct caacgcctgt
     1441 ggtgcccgga tcactgggca tggttccccg agaatcacga tcgacggggt gaagaaactg
     1501 accggttgtg agtacacgat tattcccgac cgaatcgagg ctgggacgtt catcatcgcc
     1561 ggcgccatca ccaatggcga gatcaggttg cgcaactgca ggctggagca tctcctggcg
     1621 ttggttgacc gcttgcggct gatcggcgtc aacgtggagc gagaaggcga tgaagtcgtg
     1681 gtcagcagta cccggcggct tgagccggtt gatatcacga ctcagcccta tccaggattc
     1741 ccgaccgacc tgcaagccca aaccatggct ctgctttgtc tggccgacgg caacagcatc
     1801 attaccgaga agatctaccc cgaccgtttc atgcatgtgg gcgaactgag ccgactcggg
     1861 gccaaacttc gcaaggaagg gccgacggtg atcattcaag gcgtgaagcg actggtaggt
     1921 gcccctgtca tggcctctga cttgcgagct tccgctgcct tggtgctggc ggggcttgtg
     1981 gccaagggcg aaacccgggt caaccgtgtt taccacattg accggggtta cgagcgggtt
     2041 gaacgtaggc tgcaggctct gggggccgac attgagcggc gttcaggtga ggaggagtga
     2101 ggtgccgggc gattggtcct cctgttggca tgtctgggtt cagatacggc gagttcagtg
     2161 attctggacc gaatgtaacc cactcttatg aataatctta tgacttggtc tcttcggggg
     2221 cttggactca gggctctgcc gagggggccc ataggtcgct tacctgagga acgaaggtat
     2281 cgacggattc gctgtgcccg tgccagcgag ttgtcctcgc tcgattgtcc tgacgtgggt
     2341 aggggggtgg ctgctcggct ttccacgcgg ccctgttgat atggccgacg gaaaggccgg
     2401 gtcgaggtga cttctgggtt tcgtgtcctg cgaggcctct tttgagccgt ttttctctta
     2461 gctcgattac cgctgacagg gttgacacac ttggacgtcc ggctacattg atagtaactt
     2521 ggcgagtcct cagttggcgc tgaggtcaga gtgtgccggg ttgccccacg atggctctac
     2581 cggtctgcgg ggggcagggc ctaccgacat ggaaccttgt ccgtagcctc gggcaaggtt
     2641 tgccgagaaa tccttgcgaa ggagagacat cccgtgttga gtccggatta cgacccaggc
     2701 aatcggtcga tttgcaggaa tgttctggtc tgccttgccc tggtggcgac tctgggtgtg
     2761 gccggatgcc actgcggggg cggtctgtgc gccggcgcag gtgggtctca gacgcagttg
     2821 cacttttacg cgccacctgg ggcgatggtc gagattcagg cgctgggccc aaactcgtcg
     2881 cggcaaatcc tgccgccagg cgcgttcggc gaccgcctcg agcggacgcc ggaagaggcc
     2941 tgcgtcttta acctgtcacc gggctgctac gagttcaagt acgtcggcgt cgaaggcgtg
     3001 ccgggggcgg cgatttacgg ggaactcgac gttcgccatg ccaacagctc catgggcaga
     3061 atcttccagc gccgttcggc gataccgatc gcgttgcctt cggagtatta ccggagggtg
     3121 gaagcccgcg gagatgaact cgtgccgtat cgtggcgaag ggtatcgcac ggccattgac
     3181 gagcaggaca tgatacgagt caaggccggc gacgtggtcg agaaggtttt cgtggtggca
     3241 gaccttgaga aggccgacaa gctgctccgc aagacggagg ttgagatcgc cgcggcggag
     3301 cgggagatcg aatacgccga ggcccgtttc cgcgaggctt actacaactt ccgcacggat
     3361 gtgagcgatg cctccgcgaa cttctggggc accgatcgcg agttcatccg gtgggagaga
     3421 aagcgtcagc gagctcaaca gaatctcgac aagctcatcg ctctgcgtca gcgcacgcag
     3481 tcgctgatta aggcggatcg ggtgatgatc cgtgagggaa tgctcgtgct ggccacacag
     3541 gaggtcgtcc gaccttatcg agacgtggtc gaagcggccg aggaaatcgg cgaagtgctg
     3601 ctggtcatgc ggatcggcgg tcggcacatg cagtggggtg agccgcccgg cgaactggct
     3661 gcgtacaacc catgaagcga tgaaggatct cagcccgcag gagttgagag gagccggcgt
     3721 tcgggttgct caaaaggagt gcgacgatgt ctgacaatcg gttcaggttt gcaggtgcgg
     3781 tactggccgt agttctgctg ctcgccggcg gcgtgggatg ctgcctggac aagcaagccc
     3841 gcataacggt cgcttcggcg acggatgtca ctgccggtac agcggatcgg ggcgccccgt
     3901 tgccggcagg ggtcagtctg ccaccgggtt tttatccgct ttccggacag tatgatctgg
     3961 aaaatcctga cgaccactac ggcggctggc cgcggtatat catgtgcgtg gccgacggca
     4021 tggtgatggc ctacgtgccg gcccaagagt tcatgatggg cggcggcacc gatctggatg
     4081 aggtacctgc ccgcgcggtt cgggtgaatc atttctacgt ggatattcat gaggtgacca
     4141 actgtcagtt cggtcgatat tcccggcagg ggccttacag gcagttctat atgccgggcg
     4201 tgaacgatca tcatccggtc cgcaacgtgt catgggtcga ggcccacggc tatgccgctt
     4261 gggccggcaa gagcctgccc accgaagctc agtgggaggc tgcagcgcgc gggagcgacc
     4321 gtcgcatcta tccctggggt aacgaagagc agagcgacgt aacccgctac ctgtgcaacg
     4381 ccagcaccgg ccgggactac tacgatggtt atcagggacc ggcgccggtg atgagttact
     4441 cggccggcat cagtcctttc ggggcgttca acatggccgg caacgtgtgg gagtggtgcg
     4501 ccgactggta tgatcccggt cgatatgcct atccgagcga ggaggatcct ccgtcggccc
     4561 tcgagcgagg agctttgccg ttcggtgacc ggaactaccc gagcccgaaa cataaggaca
     4621 ttcgcgaggc ccgcgtcggc ccgccggttg gcctcgagcg agtcatacgc ggcggctcgt
     4681 tcatggaccc gattcagcag tgtcgggttg acacacgagc gtcgctgccg cccgccgctc
     4741 gccgtaacaa cgtcggcttc cgttgcgtgc tgatgctgcc gccgaccgaa gacaacgatt
     4801 tggcacttgc gtctcgttga tttcgggttg cgttgccgat tcgctcagat tgacaatcga
     4861 gcatgacaga tgcaggccgg ctgctcgccg gcctgctttc tttttccgca gaagagggtc
     4921 tgggaacagt cttggggtcg ggctgctacg ttttacgtac cctttcggcc aaggacgtgg
     4981 acggggtggg gaggttgggc gaggtgttcg gggtggaacg gatgacggga aactggccac
     5041 gg
//
LOCUS       DAOTPW010000329         4998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_13919_length_4998_cov_6.059680, whole genome shotgun sequence.
ACCESSION   DAOTPW010000329 DAOTPW010000000
VERSION     DAOTPW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13919_length_4998_cov_6.059680"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2026
                     /locus_tag="P5316_20140"
     CDS             <1..2026
                     /locus_tag="P5316_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87325.1"
                     /translation="PAQLDPGPSAPMPVRDPDKEPRSASLSPAPACPAGEMITRWLMG
                     VLVALFVVTGYAPSLRFGLWFDDHAHFLHLKEAGWSHRRAVEAAYLAVPGEVMDYWGR
                     SEPLIRYYRPVAFTLMKIEYVLAGWRPEIMHGFSMGWHFLCAMLVGSLAMKFLGSRTW
                     ATVAACLMAIHPAHVGTVTWIACQTELMTTFFQLIAILAYSRHARWGELFTYSDATMV
                     GQSRGWFAGRPMEATALPSVLTADPDRRVSPAILLSLSCFAMALGCRENAVVLPVVCW
                     LGDRLCGAGRRGWLRWEHVAMAVMVIAYLCIRHWALQGFYSLTWDRMAGISHLEMIRF
                     LFDKPVVYLTGIFCCVPIVPGLTVGQPGGPPLALYAVAVAMFAAFLVIYRLFGRSPAV
                     LWPVAAMLVTMAPTIPIYPSAHHLYLPSVGGVILMTSFMALVGGIRRAPGQRLSRFRK
                     WVLGAILASHAAGLTIVTVWRGHGLEVGTLPEDLLVEDVISRSPKPIRSGDHLFFINV
                     PRMAALYPSCAVRARNGLSELYAHGLTYSPDALIMQAPGELQVLDRHRLRVRAPRESP
                     YLGGMGGRVLRSMLGLPELKTGDTFRADLDRYEVKRDADDAVSGGDWYTVVIKEADDE
                     GIRELEFVFNRPLDSPDYHFYFGSPQFMAYPVDVSRPTIAVSRPATEPAR"
     gene            2054..3355
                     /locus_tag="P5316_20145"
     CDS             2054..3355
                     /locus_tag="P5316_20145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRR87326.1"
                     /translation="MFAWLTLAVLVVNPSGSTTFDDGFYLKGGETVVFFGDSITQAGT
                     YVEYVEAFLLTRFPEKRFRIVNRGVSSETISGTSEPDHDPVRPCAHDRFTRDVVFEKP
                     DVIVACFGMNDGNYHPFDEERFDRFKRGVRRLIDRTRTETQARLVILTPPPFDPYRRL
                     VLDPEAKTYGYKYAAVDYDATLEQYSRWLMGLKEKDVLVVDLHSAMNEHLKKRRATRV
                     SFGFQQDGIHPDATGHWLMAQTLLLAWKAPGMVAELHLDASRGVAADGRVTNIRKEGN
                     SLSFDWRTPLPMPVDSRWDAESIRIEDVRRRLNRYRLAVTGLPAGRYRLTADGKEFAT
                     VSAEQLADGLDLPDYDAFPANRAAARLLSLVHDRQQRLYVAWRRGVGTWPSTDNTIWE
                     RVSVEDLRQKADELDQQIDVIRRSAPIHIGIEPVLSASSPT"
     gene            3684..3959
                     /locus_tag="P5316_20150"
     CDS             3684..3959
                     /locus_tag="P5316_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87327.1"
                     /translation="MRKDGRFRLDNCFQSTAIRAGGKPIHPAGVTYCTRGHFDHTERD
                     DDTDCTVLGVHCLKPHGPEFATANIAHQWQMRLPCLMTCTDKWASAR"
     gene            3956..4063
                     /locus_tag="P5316_20155"
     CDS             3956..4063
                     /locus_tag="P5316_20155"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRR87328.1"
                     /translation="MICVTVMGGWDTDCTGATAGSVLRAMLEADRLPAK"
     gene            4097..4624
                     /locus_tag="P5316_20160"
     CDS             4097..4624
                     /locus_tag="P5316_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87329.1"
                     /translation="MIGYNDVRITDIADAALKVNRKDQRSGFPASLKLRRGKPGTPLL
                     SERRRRGSGKDEGPRMKDEIQLHDGTNQPPTLEYARAGGRGQPPLRWWIAFVAILLAF
                     LAPRCACGYYGLISLPFTVLAAGLACYGWIGNKRKNLIWRAVLMLVVILTSITLFRNI
                     ADVLWFGHDAIWRRL"
     gene            4599..4853
                     /locus_tag="P5316_20165"
     CDS             4599..4853
                     /locus_tag="P5316_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87330.1"
                     /translation="MMRSGGGCDYRCVVPTPSSALFPLTINRANTQRPLCVVIRTCLL
                     PYQNVTNVQATFTAIARPEVDYFCEPSTRSINSLASLIAR"
BASE COUNT          879 a         1479 c         1603 g         1037 t
ORIGIN      
        1 gcccgcgcag ctcgatcctg gaccaagtgc gcctatgccc gtaagagatc ctgacaagga
       61 accgcgcagt gcaagccttt cgcccgcccc tgcctgtccg gcaggcgaga tgatcacgcg
      121 gtggctgatg ggggtcctcg tagccctttt cgtcgtcacg ggttacgctc catcgctgcg
      181 attcggcctg tggtttgacg atcacgcgca ttttctgcac ctgaaggaag ccggctggag
      241 tcacaggagg gccgtggagg cagcgtatct ggccgttccc ggcgaagtca tggactactg
      301 gggcagatcc gaaccgttga ttcgctacta tcggcccgtc gcgttcacgc tgatgaagat
      361 cgagtacgtt ctcgccggct ggcggccgga gatcatgcat ggtttctcca tggggtggca
      421 cttcctgtgc gccatgctgg ttggttcgct tgccatgaag ttcctgggca gccggacctg
      481 ggcaacagtg gcggcctgcc tcatggcgat tcacccggcg catgtcggca cggtgacatg
      541 gattgcctgc cagacggaac tgatgaccac gttttttcag ttgatcgcca tcctggctta
      601 cagccgccac gcccgctggg gggaactgtt cacctattcc gacgcgacga tggtcggtca
      661 aagtcggggg tggttcgccg ggcggccgat ggaagcaacg gcgttgcctt cggtgttgac
      721 ggccgacccg gatcgccgtg tttcgccggc tattctgcta tcgttgtcgt gctttgcgat
      781 ggctctgggc tgtcgcgaga acgccgtcgt gttgcccgtc gtctgttggc tcggcgatcg
      841 tctgtgcggc gccggcaggc ggggatggct gcgatgggaa catgtcgcga tggcagtcat
      901 ggttatcgca tacctgtgta tccggcattg ggccttgcag ggcttctaca gtcttacttg
      961 ggacaggatg gccggcattt cgcatctcga gatgatcagg tttttgtttg acaaaccggt
     1021 cgtctatttg acgggcattt tctgctgcgt gccgatagtt cctggtctca cggtggggca
     1081 accaggcggg cctccgttgg cgctttatgc agtggccgtc gccatgttcg cggccttcct
     1141 ggtcatctac cgactattcg gccggagtcc ggcggtgcta tggccggtcg cggctatgct
     1201 ggtgaccatg gcaccgacca ttcccatcta tccgtcggcc caccatctct atctgcccag
     1261 cgtcggcgga gtcattctca tgacttcgtt catggccctg gtgggcggga tacgacgtgc
     1321 gccggggcaa cgcctgagcc ggtttcggaa atgggttctc ggcgcgatcc tggcctccca
     1381 tgccgcgggg ctgacgatcg tcaccgtgtg gcgcggtcac ggattggagg tgggtacgct
     1441 gccggaagac ctgctcgtcg aagacgtcat cagccgtagc ccgaaaccga tacgatcggg
     1501 cgatcatttg tttttcatca acgtgccgag gatggccgcc ctgtaccctt cctgtgccgt
     1561 gcgggccagg aacggtttga gcgagttgta cgcccacggg ctgacctatt cgccggatgc
     1621 attgatcatg caggcccccg gcgagctgca agtgctcgac aggcaccggc tgcgggtacg
     1681 ggcgccgagg gaatcgcctt atctcggagg gatgggcgga agggtgcttc gaagcatgct
     1741 cggtttgccg gaactgaaaa ccggcgatac cttcagggcg gatctggatc gttacgaggt
     1801 caagcgagac gctgatgatg ccgtctcagg cggcgactgg tataccgtgg tcatcaagga
     1861 agccgacgat gagggcattc gcgaactgga gttcgttttc aacaggccgc ttgactcgcc
     1921 cgactaccac ttctacttcg gttcgccgca gttcatggcg tacccggtag acgtgtcgcg
     1981 gccaacgatc gccgtcagtc ggccggcgac cgagccggct cgctgagaac gtgttcgcac
     2041 gtggagaacg atcgtgtttg catggttgac tcttgccgtt ctggtggtga atccgtccgg
     2101 gtcgacgaca ttcgatgacg gattctatct caaggggggt gaaacggtcg ttttcttcgg
     2161 cgacagcatc acccaggcgg gcacctatgt tgagtacgtc gaggcgtttc tgctgacgcg
     2221 ctttccggag aagcgttttc gcatcgtcaa ccgcggagtc agcagcgaga cgatttccgg
     2281 caccagcgag ccggaccatg acccggttcg tccgtgcgcc catgatcgct ttacgcgcga
     2341 cgtggtcttt gagaaacccg atgtgatcgt ggcctgcttc gggatgaacg acggcaacta
     2401 ccatccgttc gacgaggagc ggtttgacag attcaagagg ggtgtgcgcc ggttgattga
     2461 tcgtactcga acagagacgc aagcccgcct ggtgattctg acgccgccgc cattcgaccc
     2521 ctaccgtcgg ttggtgctcg accccgaagc caagacctac gggtacaagt atgcggccgt
     2581 cgattacgac gcgacgctcg agcaatacag ccgctggctc atgggactga aggaaaagga
     2641 cgtgctcgtg gtggatcttc actcggccat gaacgagcac ctcaagaagc gccgcgcaac
     2701 gcgggtcagc ttcggcttcc agcaggacgg catccaccct gacgctaccg gccactggtt
     2761 gatggctcag acgctgttgc tggcctggaa ggcccccggc atggtcgcgg agcttcacct
     2821 tgacgcctcg cggggcgtgg cggctgacgg ccgcgtcacg aatatcagaa aggaaggcaa
     2881 cagcctgtcc tttgactggc ggactcctct gccgatgcct gtggactcga ggtgggatgc
     2941 cgagtcgatt cgcatcgagg acgtgcgccg tcgattgaac aggtatcggt tggccgtgac
     3001 aggcttacct gccggccgtt accggctcac ggccgacggg aaggagtttg ccacggtcag
     3061 cgccgagcaa ctggccgatg gtctcgacct gcccgactat gacgcgtttc ccgcaaaccg
     3121 cgcggccgcg aggcttctgt cactggttca cgatcggcaa caacggctct atgtggcttg
     3181 gcgacgcggc gtgggcacat ggccctcgac cgacaatacc atatgggagc gagtttccgt
     3241 ggaagacctc agacagaaag ccgacgagct tgatcaacag atcgatgtta tcaggcgatc
     3301 tgcaccgata catatcggca tcgagccggt cttatcggcg tcgtccccga cgtaggggcg
     3361 gttttgtatg gggtccacgc ccgcggatcg catgactgaa ccgcgcattg aacccatgga
     3421 ggcgctcaaa tctcagatga tgagccagga aaggtcgaga gggtgttgta agagacttgc
     3481 ttgccgtcat cctccttggc gcgggaccct gtgttcattc ccatcgccgg ccggcggccg
     3541 ccgcggggct cggcgaatca aggcgccgat atcagccgcc tggccgcgcg accggctgcg
     3601 cgggggcatt cctgggacgt tgcgggggtg gtctgtcggc caaaccggtg gagaacgggc
     3661 atcgcgacgg gatcttcaag gccttgagga aagacggccg cttccggttg gacaactgtt
     3721 tccagtcgac cgccatccgg gcggggggca agccgatcca cccggcggga gtgacatact
     3781 gcacccgcgg ccatttcgat cacacggaac gcgacgacga taccgactgc accgtactcg
     3841 gtgttcattg cctcaagccc catggtccgg agttcgccac cgccaacatc gcccaccagt
     3901 ggcagatgcg attgccctgt ttaatgacct gcacggacaa atgggcttcg gcaagatgat
     3961 ctgtgtgacg gtgatgggcg gctgggatac cgattgcacc ggcgccaccg ccgggtccgt
     4021 cctgcgggcc atgctggagg ccgatcgcct gcctgccaag tgaacccggc cactgaacaa
     4081 ccgcgttgag tcaatcgtga ttggatacaa cgacgtgagg atcaccgata tcgcggacgc
     4141 ggcgctgaag gtgaatcgaa aggatcagcg ttcggggttc ccggcttcgc tgaagcttcg
     4201 ccgtggcaag cccggcacgc cgttgctttc tgagcgaagg cggcggggct caggaaagga
     4261 tgaaggccca aggatgaagg atgaaatcca gttacatgat ggcacgaacc agccgccgac
     4321 gctggaatac gccagggcgg gtggccgagg ccagccaccc ctgcgctggt ggatcgcgtt
     4381 tgtggcgatt cttctggcct tcttggcacc ccgctgtgcc tgcggctatt atggcttgat
     4441 cagtctcccg tttacggtgc tggcggcagg actcgcctgt tacggctgga tcggcaacaa
     4501 acgaaagaac ctgatctggc gcgccgtctt gatgctggtc gtcatcttaa cttccatcac
     4561 cttgttcaga aacatcgctg atgttctgtg gtttgggcat gatgcgatct ggaggaggct
     4621 gtgactacag atgcgtggtg ccgacgccct cgtcggcact cttccccctg acaatcaacc
     4681 gtgccaatac gcaacgcccg ctctgcgtgg tgattcggac gtgtcttctg ccgtaccaga
     4741 acgtcaccaa cgtccaggca acgttcacgg ccatcgctcg gccagaggtc gattacttct
     4801 gcgaaccaag cacccgctcg atcaattccc tggcatcgct catcgcgagg tagaggtgct
     4861 tctggtcgtc cggcaggcgg gcaaagccga tatgtttgca ggccgctcag catcaccccg
     4921 cgcaagccgg gtccgcgggt atccccgggc cggtcaggca ggccctgaag atcgcgatgt
     4981 cgtcctggtc cacgtcgt
//
LOCUS       DAOTPW010000330         4920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14176_length_4920_cov_5.984378, whole genome shotgun sequence.
ACCESSION   DAOTPW010000330 DAOTPW010000000
VERSION     DAOTPW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14176_length_4920_cov_5.984378"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="P5316_20170"
     CDS             <1..736
                     /locus_tag="P5316_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87331.1"
                     /translation="DYWASSSGINRTRAVANTGSYDFSMWSWCGQASSYSEAQIANYL
                     SVMAQFEDEYPGMRFILMTGHTDGSGVGGTLNQRNNQIRAYALAHNMVLFDFADIESH
                     DPDGNDYLALGCNDNGDYSGGNWPQQWCAAHPGHPLCTSCSCAHSQPTICNLKGRAFW
                     WMMARLAGWPGPVAPDLDGDRDVDNDDFALFEACASGSGVSHGGSSTCSQADFDGDTD
                     VDQSDFGIFQRCMSGENVQADPLCDD"
     gene            968..1381
                     /locus_tag="P5316_20175"
     CDS             968..1381
                     /locus_tag="P5316_20175"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HRR87332.1"
                     /translation="MAEDNKEAPKIIIDDDWKEQARREKEEADREARAAEETERGPLP
                     GPHIAEIIQMITMQATIGLGGFRDQNGKTIPPSLEYAKHYIDLLELLQNKTRNNLDDQ
                     EQRILTGTLQELRLAFVEVYEAMLRQAASPPPPKK"
     gene            1417..2625
                     /locus_tag="P5316_20180"
     CDS             1417..2625
                     /locus_tag="P5316_20180"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRR87333.1"
                     /translation="MIIPISTDTQIRRTPWANYVLIGLNVLVFLVVGGADRHAIDGRQ
                     TIGDRLALVGDHIQLYQFFTYQFMHANWMHIGGNMLFLWIFGNAVNGKMGHAPYVLFY
                     LAGGIAAAVGHAYINPDFGGMVGASGAIAAVTTAYLALFPRSYVTVLYWWFIIGTFEL
                     PSMVLIVFKMILWDNVLAKSLAGESNVAFDAHLAGYGFGFVATSALLAIKALPRDQFD
                     IVALWRRWFHRQAFRSAMADPDAEARARYGRVARPVSLQDLPVGSGASGPRDQVAELR
                     MRIAEALALNDRNTAAELYEKLLEHDPRQVLPKGSQLDVANQLYTLNKLPQAAAAYEK
                     LLSAYPTCAEAGEIRLLLGIIYARDLRQYEVAEGHLRQALVQFADEKRREQCRHWLAV
                     AADGLGRNEE"
     gene            2890..4002
                     /gene="aroB"
                     /locus_tag="P5316_20185"
     CDS             2890..4002
                     /gene="aroB"
                     /locus_tag="P5316_20185"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRR87334.1"
                     /translation="MSSASGERVVQVNVEPRYPVFIGAGLLGALGPRLAERVKSRRAM
                     VITDENVGPLYGKTAMDSLRAAGFDAATFVVPAGDGSKSLEWVSRIYDALADARIDRT
                     SPLIALGGGVVGDLTGFVAATWVRGVPFVQCATTIEADVDASVGGKTAVNHASGKNMI
                     GAFYQPLFVLIDTATLRTLSDRDFKAGLAESIKHAVIRDADFFAWHEDNADHVLACDT
                     DALGALIEHNVRIKADIVSQDERETSGLRALLNFGHTVGHAIESLMARQGDPWRHGEA
                     VAAGMVAAAEISVAAGRLDRAAAERIAAIVDRMKLPVTAPLAGQRDRIISLMQSDKKV
                     AAGKLRFVLADCIGRATLYDDIKPEWIEAGLDRVLR"
     gene            complement(4232..4429)
                     /locus_tag="P5316_20190"
     CDS             complement(4232..4429)
                     /locus_tag="P5316_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87335.1"
                     /translation="MRSQYDFTNAIRGKYAAEYAQGTNLVALSDEVARVFPDSHAVNE
                     ALRTLIRLGTARRARSARGDR"
     gene            complement(4431..4724)
                     /locus_tag="P5316_20195"
     CDS             complement(4431..4724)
                     /locus_tag="P5316_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRR87336.1"
                     /translation="MNFEWDAAKARSNRRKHEAAFEEAATVFGDPRSLTIYDPDHSIV
                     EERFVTMGLSAAGRVLVVDHTDRAKRIRLISARKATQRERRAYETEREDPLIG"
BASE COUNT          944 a         1539 c         1558 g          879 t
ORIGIN      
        1 tgactactgg gcgagttcgt ccggtatcaa caggacgcga gcagtggcca acaccggctc
       61 gtatgacttc tcgatgtggt cgtggtgtgg acaagcctcg tcgtactctg aggcgcagat
      121 cgcgaactac ctcagcgtga tggctcagtt cgaggatgag tacccgggca tgcgtttcat
      181 cctgatgacc ggccacaccg acggcagcgg agtgggtggt accctgaacc agcgcaacaa
      241 tcagatccgt gcgtatgcac tggcccacaa tatggtgctg tttgactttg cagacatcga
      301 aagccacgac cccgacggca atgactacct ggccctgggc tgcaacgata acggtgacta
      361 cagcggaggt aactggcccc agcaatggtg tgccgctcac ccggggcatc cgctctgcac
      421 gagctgctcg tgtgcgcatt cccagccgac gatctgcaac ctcaagggcc gcgcattctg
      481 gtggatgatg gctcgactgg ccggctggcc cggtccggtc gccccggatc tcgacggtga
      541 ccgcgacgtt gacaacgacg atttcgctct cttcgaggcc tgtgcctccg ggtcaggcgt
      601 gtcacacggc ggcagttcga cctgcagcca agcagatttc gacggtgaca ccgacgtcga
      661 ccagtccgat ttcggcatct tccagcgatg catgagcggc gagaacgtcc aggccgaccc
      721 cctttgcgac gactgatgaa cgggaacccc cgccaagtcc acggggggcg gcggcggatc
      781 agccccagca cgaccgcctt gtccgccttt gtgaagggcc gccgagggat tgcacgtcga
      841 tgcagcagcc cgggcggcta tcgacggact ggacatcatg ctatgatctg aggtccgacc
      901 ggtcgggttg ccggctcggg acgagtgctt gtttcagcca gacttaacga acacaacgga
      961 ggccaccatg gccgaggaca acaaggaagc cccgaagatc attatcgacg acgactggaa
     1021 ggaacaggct cgtcgcgaaa aagaggaggc cgatcgcgag gcccgcgcgg ccgaagaaac
     1081 agagcgcgga ccgttgccgg ggccgcacat cgccgagatc attcagatga tcaccatgca
     1141 ggcgacgatc ggcttgggcg gcttccgcga ccagaacggt aagaccattc cgccgagcct
     1201 ggaatatgcc aagcactaca tcgacctgct cgaactgctg cagaacaaga cccgtaacaa
     1261 cctcgacgac caggaacaac ggatactcac cggtacgttg caggagctca ggctcgcctt
     1321 cgtggaagtg tacgaggcga tgctccggca ggccgcttcg cctccaccgc cgaagaaatg
     1381 acccgtttgc gttgcagctc cggaaccatg tgatccatga tcattccgat ctcgacagat
     1441 acgcagattc gccgcacgcc gtgggccaac tacgtgctca tcgggctcaa tgtgcttgtt
     1501 tttctcgtgg tgggcggcgc ggatcgccat gcaatcgacg gcaggcaaac gataggcgac
     1561 cggctcgcgc tggtgggtga ccacatccag ctctaccagt tcttcaccta ccagttcatg
     1621 cacgccaact ggatgcacat cggcggcaat atgttgtttc tgtggatttt cggcaacgcc
     1681 gtcaacggca agatgggcca tgcaccctac gtactgttct acctggcggg cggaatcgcc
     1741 gcggcagtcg gtcacgctta catcaacccc gacttcggcg gtatggtcgg ggcctccggt
     1801 gccatcgcgg ccgtgaccac cgcctacctg gccctttttc cgcgcagcta cgtcaccgtc
     1861 ctgtactggt ggttcatcat cggcacgttc gaattgccca gcatggttct gatcgtgttc
     1921 aagatgatcc tgtgggacaa cgtcctggca aagagcctcg ccggcgagag caacgtggcc
     1981 ttcgatgccc acctggcggg ctacggcttc ggctttgtcg cgacgagcgc cctgctggcg
     2041 atcaaggcgc tgccgcggga tcaattcgac atcgtggccc tgtggaggcg atggttccac
     2101 cgccaggcct ttcgctcggc gatggccgat ccggacgccg aggcccgggc ccgctacggg
     2161 cgagtggccc ggccggtgtc gctccaggat ttgcccgtcg gcagcggggc atccgggccg
     2221 cgcgaccagg tcgccgagtt gcggatgaga attgccgagg ccctggcgct gaacgatcgg
     2281 aacacggccg ccgaactgta tgaaaagctg ctggaacacg acccccggca ggtgctgccc
     2341 aagggcagcc agctcgatgt ggccaaccag ctctacacgc tcaacaagct gccccaggcg
     2401 gccgcggcgt atgagaagct gctgtcggcg tacccgactt gcgcggaagc tggcgagatc
     2461 cggctgttgc tgggcatcat ctatgcccgg gacttgcggc aatacgaggt cgccgagggc
     2521 cacctgcgac aggcactggt ccagttcgcc gacgaaaaac gccgggagca gtgccggcac
     2581 tggctggccg tggcggcgga tggcctcgga aggaatgagg aatgatgact tgagaatggc
     2641 gaatcaaaaa aaccatacgc ccgtctctct gagctgctgc caacgccttt tgacttccag
     2701 aatccgtgtg caaagggtag gggacgatcc gagccgccgg aggcggcgga gacctgccgg
     2761 ttcgagctca acaatatggc aggtctcggt cgccgcggcg acctcgacgc tgccctaccg
     2821 aatgcttgcg gcggcttcca cgcatgctct caattcccaa ttcggtattc tcaatttggg
     2881 gtctggctaa tgagttcggc gtctggcgag cgtgttgtcc aggtcaacgt tgagccgcgc
     2941 tatccggtgt tcatcggggc gggactgctc ggcgcactgg ggccgcgcct ggccgagcgg
     3001 gtcaagagcc gccgggcgat ggtgatcacc gacgagaacg tcgggccgct ctacggcaag
     3061 acggcgatgg actctctgcg ggctgccggg ttcgacgcag ccacgttcgt cgtgcccgcc
     3121 ggcgatggca gcaagagcct tgagtgggtc tcgcggatct acgacgctct ggccgacgcc
     3181 cgaatcgacc gaacctcgcc gctgatcgcg ctaggaggcg gggtggtcgg cgacctgacg
     3241 gggtttgtcg cggcaacatg ggtgcgcggc gtgccgttcg tgcagtgtgc gaccaccatc
     3301 gaggccgacg tcgacgccag cgtcggcggc aagacggcgg tcaatcacgc cagcggcaag
     3361 aacatgatcg gggcctttta ccagccgctg ttcgtgctca tcgacacggc cacgctgcgg
     3421 acgctgagcg atcgcgattt caaggctggt ctggccgaga gcatcaaaca cgcggtcatc
     3481 cgcgatgccg acttcttcgc ctggcacgaa gacaacgccg accatgtcct cgcctgcgac
     3541 accgacgcac tgggggccct gatcgagcac aacgtccgca tcaaggcgga tatcgtctcg
     3601 caagacgagc gcgagacgag cggcctgcgg gcgctgctga acttcgggca caccgtcgga
     3661 cacgcgatcg agtcgctgat ggcacggcag ggtgatccgt ggcggcacgg cgaggcggtg
     3721 gccgccggca tggtcgcggc cgcagagatc agcgtcgccg ccggtcgcct ggaccgcgcg
     3781 gccgccgagc gcatcgccgc gatcgtcgac cggatgaagc tgcccgtgac ggcccctctg
     3841 gccgggcagc gcgaccggat catcagtctg atgcaatccg acaagaaggt cgccgccggc
     3901 aagctgcgat tcgtcctcgc cgactgcatc ggccgggcaa cgctctacga cgacatcaaa
     3961 ccggagtgga tcgaagcggg gctggacagg gtgctgagat agggttcccg ccttcgccaa
     4021 ggcttcggcg ggcaagcagg attcaggatg gcaggggtct tcgaccgcaa ttcttgacga
     4081 gccgaggggt ttatcccctc ggtctttcgg taatgcgcag gtccaaccgg cgggacaaaa
     4141 gcacgggctg gtcaagaaat cgggcgtggg gcaccgcgat gatgccccac gcccgttgtt
     4201 gttcaagccg caggtgttac ggccatctca gtcagcgatc acctctggcg gagcgtgctc
     4261 gccgggcagt cccaagacga atcagcgtgc gcagggcctc gttgaccgca tgggaatcgg
     4321 gaaaaacgcg ggcgacctca tcggacaagg cgaccaggtt cgtcccctgt gcgtattcgg
     4381 ccgcgtactt gccgcggatg gcgtttgtga aatcgtactg acttcgcatc tcacccgatc
     4441 aacgggtctt cccgttccgt ttcatacgct cttcgctccc tttgagtcgc tttgcgggcg
     4501 ctgatcaacc gaatgcgttt ggcgcggtcc gtatgatcaa ccacaagtac gcgtcccgct
     4561 gccgacaacc ccattgtaac aaaacgctct tcaacgatcg agtgatcagg atcataaatc
     4621 gtcagcgatc tcggatcacc aaacaccgtg gcagcctctt caaaggccgc ctcgtgtttg
     4681 cggcggttgg accgcgcctt ggcggcatcc cattcgaagt tcaagcccat tcggctacac
     4741 ctctggagaa ctgctgctca tggaatcggt atctccagcc ggcatgtgta tacatctaca
     4801 ccacagctcc cacaagtcgc gcaaggagcc ggcaccgaaa accacgacga gatcgtggta
     4861 cgtgtctagc ccaagttgaa cactttcgag gcaagaatgg ccttggcgat agcgccaggg
//
LOCUS       DAOTPW010000331         4917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14185_length_4917_cov_5.482106, whole genome shotgun sequence.
ACCESSION   DAOTPW010000331 DAOTPW010000000
VERSION     DAOTPW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14185_length_4917_cov_5.482106"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1181)
                     /locus_tag="P5316_20200"
     CDS             complement(3..1181)
                     /locus_tag="P5316_20200"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HRR87337.1"
                     /translation="MPWLAISLTLTGLYAQEPTRGIETFDGPIVFSQEQIILARTWEL
                     TGDLGVKAVTPAGETLVVPAKDALLLETGAATLPGFRPRVVLRNGESIIAEPQAGGND
                     RRWSFTSPLWKSLQLDGSRIARYQAAPVSLAGDHPAPPFVLLRNGDVVAAGVDRISDG
                     QVTVNTEFGQTHIPLETVSAIILAADSATTDGREANQFLVELADGERLYCDRIGESDQ
                     DVVLERSGSRCRVGREAILRVVWPGTALTPLTQLPLRGDGRAYFGAPALPRKDRNALG
                     GPLRIGDRWFERGLGMRPRSRCMFQLSGRWMYLTGHVGLDPWLGRRGDCQVAVCLSGK
                     EACKEMLQGGQKGRRLLLPLAEAKDIELQVDFGPGGDLGDYVNWCDLMLIGPRGENSK
                     "
     gene            1364..2551
                     /locus_tag="P5316_20205"
     CDS             1364..2551
                     /locus_tag="P5316_20205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87338.1"
                     /translation="MHTELTRRGFIKQVAIGASVAAVPTVARGYPANERIRLGWIGIG
                     NRGEQLLTEVTTQFVREACTVAVCDLLADRVAKGLQLAALDRPKGYTDMRTMIEHERL
                     DAVIVATPPATHAEVSIPVMQAGIHCLQEAPLETTVEKIDALSRVARKAKVVFQIGAQ
                     RRYHPGFTAALPRLLKGEFGKITFMQGHWHWPWALLTHPIDRTGGMFLDQACHHFDVM
                     CWAMNEKPPITCTAMGYHQARPKAGPKAFSPTHATASFRFPDGRLFAYTHLFCLPKRF
                     TDEQLQIFCENGCLDLIRGMFYDADSTEKRIGEASGDDSMKGTSEMLRDFFAVIRDGG
                     GRAPRASLETARNAALMGIMGRMAMSSPSGDAYEPRVIHWADLQTTTDPETKPASTPS
                     GNG"
     gene            complement(2639..3898)
                     /locus_tag="P5316_20210"
     CDS             complement(2639..3898)
                     /locus_tag="P5316_20210"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRR87339.1"
                     /translation="MKGRILVVTSAIALGLLAGCRTGPRFDGPVRAVWVVRTDYGSPE
                     DVKQVMANCADAGFNNVIFQVRGNGTVYYPSKIEPWSEEYDYKSPGFDPLAVACREAH
                     GRKLKIHAWVNVMPAWKGEAPPKCPDQLYNKHPEWFWYDQHGRRQPLNSFYVSLNPCL
                     PEVRTYIVDVFRELLANYPLDGLHMDYIRFPNEPPAIPVGSDIDYPRDKRTLELYRGE
                     SWLWPNPDDNKANWDRWRTRQVTKLVAQIRDMVRETRPQAILSASVGTNRRQSLSHFR
                     DELYWARWGLIDAAFPMNYKPDVQKFDQGLEMWMPYRRQIDVVPGLWFDGKLPTEQGI
                     SVVRQQIASAVDKTGNFCLFSYTSLFDPPAGRRDQRTRPTDSARPRARGTGQRELRRH
                     QLVPYIRSLGSSTADRVAMTNAGPVAK"
     gene            4281..>4917
                     /locus_tag="P5316_20215"
     CDS             4281..>4917
                     /locus_tag="P5316_20215"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HRR87340.1"
                     /translation="MAAPSDKRGYNDVYAGRYINRVAFPLGGIGAGMFCLEGTGAISH
                     LSLRHQPEVFNEPCTFAAVCVKGEKNIARVLEGPVPAWKVFGAPGSGNGAAGKTYGLP
                     RFREASFKARFPFGIVALSDSRVPLAVEITGWSPFEPGDPDNSSLPVAGLEYRFTNTS
                     KQAIDAVFSFNTRNFMAIGNDGHGVRQGHQGFVLWQKGSEDKPWEQGAFSVS"
BASE COUNT          966 a         1552 c         1520 g          879 t
ORIGIN      
        1 tctcatttgc tgttctctcc ccgcggcccg atcaacatga gatcgcacca gttgacgtag
       61 tcgccgagat cgccgcccgg cccgaaatcg acctgcagct cgatgtcctt tgcttccgca
      121 aggggcagca gcagccggcg ccccttctgc ccgccttgga gcatctcctt gcatgcctct
      181 ttgccgctga gacacacggc cacctggcaa tcgccgcgcc ggcccaacca aggatcaagc
      241 ccaacgtgcc ccgtgaggta catccacctg ccgctcaact gaaacatgca ccgcgaacgc
      301 gggcgcattc ctagaccgcg ctcaaaccag cgatcaccga tccgcagggg acccccaagg
      361 gcgttgcgat cctttcgagg caatgccggc gcaccgaagt aagctcgtcc gtctccccgc
      421 aacggcaact gcgtgagggg cgtcaatgcg gtgccaggcc aaacgacccg caggatggcc
      481 tcccggccga cgcggcaccg tgaaccgctg cgttcaagaa cgacgtcttg gtccgactcg
      541 ccgatcctgt cgcagtacaa tcgttctccg tccgccagtt ccaccaggaa ctggttcgcc
      601 tcgcggccat cagtcgtcgc cgagtcggcg gccaaaatga tggccgagac cgtttcgagt
      661 ggtatgtgag tctgaccgaa ctcggtgttg acggtcactt gtccgtcgct gatccggtcg
      721 acaccggcgg ctaccacatc accgttgcgc agcagcacga agggcggtgc cggatgatcg
      781 ccggcgagcg agaccggtgc agcctggtag cgggcaatcc gggaaccatc cagttggagg
      841 gacttccaga gcgggcttgt gaatgaccag cgccgatcgt tgccgccggc ctggggctct
      901 gcgatgatcg attcgccgtt acggagcacg acacgaggtc gaaagccggg cagtgtggcc
      961 gcgccggtct ccaagagcag ggcgtccttc gccgggacaa cgagcgtttc gcccgccgga
     1021 gtgaccgcct tgacacccag atcgccggtc agctcccacg tcctggccag gatgatctgc
     1081 tcctgagaaa aaacgattgg gccgtcgaag gtctcgattc ctcttgtcgg ctcttgggca
     1141 tacagaccgg tcagcgtaag tgagatcgcc agccaaggca tgaacgcgtc tccgaatggt
     1201 cgcaagactc cgcgcggtaa gtcgcccgta cttgtacaca atcaaagaca tcgaagcaat
     1261 acccgcttgg ggataatgac cggtggccca ttgctgtttg actgtcggcg gcggatgggt
     1321 caggatgatg gaaaccacgg gaggcaagac ggggagaccg aacatgcaca cagaactgac
     1381 acgccggggc tttatcaaac aggtcgccat cggggcgtcc gttgcggccg tgcccacggt
     1441 ggcccgggga taccctgcca atgagagaat ccgcctgggc tggatcggga tcggcaatcg
     1501 gggcgagcag cttctgacgg aggtgacgac ccagttcgtg cgagaggctt gcaccgtggc
     1561 ggtctgcgac ctgcttgctg accgcgtcgc caagggcctt caattggcgg ccctagaccg
     1621 gccgaaaggc tataccgaca tgcgcaccat gatcgagcac gagcgcctcg atgcggtgat
     1681 cgtggcaacc cccccggcga cgcatgccga ggtttcaatc ccggtcatgc aggcgggcat
     1741 ccactgtctc caggaggccc cgcttgagac gaccgtcgag aagatcgatg cgctcagccg
     1801 cgtagcccgc aaggcgaagg tggtattcca gatcggggca cagcggcggt accaccccgg
     1861 gttcaccgcg gccctgccgc gtctgctgaa gggtgagttc gggaagatca ccttcatgca
     1921 gggccattgg cattggccgt gggccctact gactcatccg atcgacagga cgggcggcat
     1981 gttcctcgat caggcctgcc atcacttcga cgtgatgtgt tgggccatga acgagaaacc
     2041 gccgatcacc tgcaccgcca tgggctatca tcaggcgcgt ccgaaggccg gccccaaagc
     2101 cttcagcccc acgcatgcta cggcttcctt ccggtttccc gacggcagac tgttcgccta
     2161 cacacacctc ttctgccttc ccaagcggtt caccgacgag cagttgcaga tcttctgcga
     2221 gaacggttgc ctggacctga ttcgcgggat gttctatgac gctgacagca ccgagaaacg
     2281 gatcggagag gcaagcggtg acgattcaat gaaaggcaca agcgagatgc tcagggattt
     2341 cttcgccgtc atccgcgacg gcggcggccg cgccccgcgg gcgagtctgg aaacggctcg
     2401 caacgccgcc ctgatgggca tcatggggcg aatggccatg agcagcccgt ccggcgatgc
     2461 ctacgaaccc cgcgtgatcc actgggccga cctgcaaaca acgaccgatc cggaaacgaa
     2521 accggcgtca accccctcgg gaaacgggtg agagaaggcc tccgctgcta cagcccctcc
     2581 tgcaatcacg aggccgcgag gtgatgtatt ggtgagctgg caggatccag gttggccctt
     2641 atttggcgac aggcccagca ttggtcatcg ccactcggtc tgcagtcgag ctgccgagcg
     2701 aacggatgta gggcaccaac tggtgtcggc ggagttcgcg ttgaccggtg cctcgggcgc
     2761 gcggtcgagc ggagtcagtg ggtcgcgtcc tttgatcgcg ccgaccggca ggcgggtcaa
     2821 agagcgacgt ataggagaac agacagaaat tgcccgtctt gtccacggcg gacgcgatct
     2881 gctgtcggac gactgaaatg ccttgctccg tcggaagctt gccgtcaaac cacaagccgg
     2941 gcaccacgtc gatctgacgg cgataaggca tccacatctc cagcccctga tcgaacttct
     3001 ggacatcggg cttgtagttc atcggaaacg cggcgtcgat cagcccccaa cgcgcccaat
     3061 agagctcatc gcgaaagtgg ctcagactct gcctgcggtt ggtcccgacg gaagcggaca
     3121 gtattgcctg tggccgcgtc tcgcggacca tgtcacgaat ctgggcaacc agcttggtga
     3181 cctgacgagt ccgccagcgg tcccagttgg ccttgttgtc atccgggttc ggccacaacc
     3241 atgattcccc ccgatatagc tcgagcgtcc gcttgtcgcg cgggtaatcg atgtccgagc
     3301 ccacgggaat ggccggcggc tcgttgggga agcggatgta gtccatgtgc agcccgtcca
     3361 gcgggtaatt ggcaagaagc tcgcgaaaca cgtcgacgat gtacgtccga acctccggca
     3421 gacaagggtt gagactgacg taaaaagagt tcagcggctg acgccggccg tgctggtcat
     3481 accagaacca ctcaggatgc ttgttgtaaa gctggtccgg gcacttcggc ggggcttcgc
     3541 ccttccaggc gggcatgaca ttgacccagg cgtggatctt gagtttgcgt ccatgagctt
     3601 cccggcacgc caccgccagc gggtcgaaac ccggcgactt gtagtcgtac tcttccgacc
     3661 acggctcgat cttggacgga tagtaaaccg tgccgttgcc gcgaacctgg aagatgacgt
     3721 tgttaaaccc ggcgtcggca cagttggcca tgacctgctt gacatcctcg gggctcccat
     3781 aatcggtgcg aacgacccag acggcccgga ccggtccgtc aaagcgcggc cccgttcggc
     3841 aaccggccag caggccaagc gcaatcgcgg aagtgaccac aagaattctg cccttcatga
     3901 tgaacctcca ggaaatgtcg ggtcaacatc tgacacaggt cgcccggatc gtaccagaaa
     3961 acggccatcc tggcacgcga tatcggcctg acggcgatgg cgtcctgcgg gccggttacg
     4021 ctcgtcttgc cccatccctt tgcccgggta agatggtaac ctccgccggt cgggcaggac
     4081 aaagttaggc tgcttaatct aaccccaagg agcgtaccat ggcttccgat gagatcaacg
     4141 acacatgcat gccgcgtcgg gatttcttga aaaagtccgt tgctacggcc ggagtgatcg
     4201 gtgccggggc gggcgccggg accgccgggt gctctcacca cccacacgag atcggccctc
     4261 acggaatcga ggcaggagat atggctgctc caagcgataa gcgaggctac aacgacgttt
     4321 acgccgggcg gtacatcaac cgggtggcct tccctcttgg tggaatcggg gccggcatgt
     4381 tttgcctgga agggaccggg gcgatttcgc acctctcgct gcgccaccag ccggaggtgt
     4441 tcaacgagcc ctgcaccttt gccgctgtat gcgtgaaagg cgagaagaat atcgcccgcg
     4501 tgctcgaagg tcctgtcccg gcctggaagg tcttcggggc cccgggtagc ggcaacgggg
     4561 cagccggaaa gacgtacggt ctgcctcgct tccgcgaagc gtccttcaaa gcacgtttcc
     4621 cctttggcat cgtggctttg tcagacagcc gggtaccact ggccgtcgaa atcaccggct
     4681 ggagcccttt tgagccgggc gaccccgaca actcgtcgct gcccgtcgcc ggattggagt
     4741 acaggtttac gaacaccagc aaacaggcca ttgatgcggt gttctcgttc aacacccgca
     4801 acttcatggc tatcggcaac gatggccacg gtgttcgaca gggccatcag ggattcgtcc
     4861 tctggcaaaa gggcagcgaa gacaagccct gggagcaggg cgcgttctcg gtatccg
//
LOCUS       DAOTPW010000332         4896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14258_length_4896_cov_5.601735, whole genome shotgun sequence.
ACCESSION   DAOTPW010000332 DAOTPW010000000
VERSION     DAOTPW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14258_length_4896_cov_5.601735"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="P5316_20220"
     CDS             complement(<1..202)
                     /locus_tag="P5316_20220"
                     /inference="COORDINATES: protein motif:HMM:NF014924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HRR87341.1"
                     /translation="MPMRNPGADSLLFAALFLLARGVLAAANDPMVSRPPESRAGEAE
                     TGIWIRVNQIGYLPDDPKIAVLS"
     gene            complement(207..1154)
                     /locus_tag="P5316_20225"
     CDS             complement(207..1154)
                     /locus_tag="P5316_20225"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRR87342.1"
                     /translation="MTVGWVYDRFFHRHQTGETHPERAVRLEVIVSDLTAAGLLGRMR
                     HLPFQPAPTDIVSWIHEPAYVELVRMACEQGFSFIGSSETKICPESYDAALLAVGGVL
                     AACDAAMSGEVPRSFCAVRPPGHHAERDLAMGYCLFNNVAIAAEYLVRRHGLKRIAIV
                     DWDVHHGNGIQHAFEERRDVFYISVHQMPQSLFPGTGYEHEMGRGPGQGFTLNVPMKP
                     GSGDREFHEAFDTRIFPRLDEYEPEFVLVSAGFDATRGERIASLDLEPSSYGWMTEAV
                     VEIARRHSRGRLVSVLEGGYDLTRLGRCVIEHITAMMDE"
     gene            complement(1200..2069)
                     /locus_tag="P5316_20230"
     CDS             complement(1200..2069)
                     /locus_tag="P5316_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87343.1"
                     /translation="MYFVKYLKTAFLNRWNLLLLAGAMGFALLSGRADVWVPLVLAVE
                     VGYLGMLGTHPKFQAYVDARQNAALRQQGTADVERMMKSIIAALPEKSLERYEALRAR
                     CLELRQIALAIKYPGHTGSASILEQSQLAGLDRLLWIYLRLLFTQYSLERFFQKTSEA
                     QIQQDIDSLQARLQKVSRLKDEAQRQKFIRTIEDNLETCRNRLSNYQKARSNYELIQL
                     EIDRLENKIRSLSELAVNRQEPDFISGQVDQVAASMLETERTMNDLQFATGLITVDET
                     APQLLPREEIRQQ"
     gene            complement(2081..2989)
                     /locus_tag="P5316_20235"
     CDS             complement(2081..2989)
                     /locus_tag="P5316_20235"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR87344.1"
                     /translation="MTETDNTTAAGPGKPAEPVGIGEVLPEIRKPEQLARPPVVQFSG
                     VTKTYNAGRANSYTAIRDVNFVIEDLPDKGEFVCVLGPSGCGKSTILRLVAGLEPQHP
                     PTEGRVLVLGRPVTGPGADRGMVFQDYTSFDHRTVLENVTFGLECQHRPRKERDELGR
                     HWIERVGLNVKTDADKYPHELSGGMRQRVAIARTLILKPRIILMDEPFGALDPQTRMN
                     MQDLLVALWKEVQATVLFVTHSIEEAVYLGDRVYIMSNSPGTILRELVVQPADRPSKQ
                     MQREPKFQETVAYIRDLISELEEGRD"
     gene            complement(2986..3927)
                     /locus_tag="P5316_20240"
     CDS             complement(2986..3927)
                     /locus_tag="P5316_20240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR87345.1"
                     /translation="MRKLFVLRREIPPWQAGLCGIACVVMVLGLWWLATRGEPEQRLL
                     GPTVLPSPAETFATFPSLWFDRALTRNTWASLRRVALGFGLATLMGVPLGILGGCFSW
                     FHAFLSPLSIFGRNIPVAALIPLTFSLFGIGEQQKIMFIFIACVAFIVSDSAQAVVDV
                     DRRYIDTAYTLGARRRQVILKVLVPLAMPGIFNSLRLLFGLAFGYIMLAELVKFGDES
                     GGLGDIIMTSQKRGQKEHILLVLMIIPLVAMAIDRVLFWIQRELFPHRYGSHGILNRG
                     LRLVLHVYEDFINIFRRSVAQAAISAREPASTPDKKP"
     gene            complement(4172..>4896)
                     /locus_tag="P5316_20245"
     CDS             complement(4172..>4896)
                     /locus_tag="P5316_20245"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR87346.1"
                     /translation="GYNIPAGDALGMLGDAHSTNWAENYQFFVNQNNPANFERVWRQA
                     YNLYRRIGSITHQPVSFDQVMDFSIIEKLGKEPKYASQKDEYRIQFTPASAGEIKAED
                     PILTNTVFIHFFPNSWDLHKKVTKKIDGKDVEQLYDPQVDFVLEDIAKLVGQFGAARI
                     IIQGHTDASMRGQVTPSLVKELSLNRANAVKEALVNKYKLDPNQFAVEGVGWDKPADP
                     NDPDNHAKNRRVEVKVYPAERQ"
BASE COUNT          901 a         1460 c         1589 g          946 t
ORIGIN      
        1 atgagaggac ggcgattttc ggatcgtcgg gcagatagcc gatttggttg acccggatcc
       61 agatgccggt ttcagcttcg cccgcccgcg actcgggcgg ccgcgagacc atcggatcgt
      121 tcgcggcagc cagtaccccg cgggccaaca ggaagagtgc cgcaaacaga agactgtcag
      181 ctcctggatt ccgcatcggc atcggtctac tcatccatca tagccgtgat gtgctcgatc
      241 acgcatcgcc ccagtcgcgt aagatcgtac ccgccctcga gcacgctgac cagccgaccg
      301 cgggagtgtc gcctggcgat ctccacaacc gcctcggtca tccatccgta tgacgacggc
      361 tccagatcga gggaggcgat gcgctcgcct cgcgtcgcgt cgaagcctgc gctgaccagg
      421 acaaattcgg gctcgtattc gtcgagtctg ggaaagatcc gggtgtcgaa ggcctcgtgg
      481 aactcgcggt cgccgctgcc gggcttcatg gggacgttga gggtgaaacc ttggccgggc
      541 ccgcgcccca tttcgtgttc gtaacccgtt ccggggaaca gcgattgcgg catctggtga
      601 acactgatgt agaacacgtc gcggcgctcc tcgaaggcat gctggatacc attgccgtga
      661 tgaacgtccc aatcgacgat cgcgatccgt ttgaggccgt ggcggcgaac caggtactca
      721 gcggcgatgg caacattgtt gaacaggcag tagcccatgg ccaggtcgcg ttcggcatgg
      781 tgtccgggcg gtcgaaccgc gcaaaacgac cgcgggacct cgccggacat ggcagcgtcg
      841 caggcggcca acacccctcc gacggccagg agggcggcgt catagctttc cgggcagatt
      901 tttgtctcag acgaaccgat gaagctgaaa ccctgttcgc aggccattct caccagctcg
      961 acgtaggcgg gctcgtggat ccagctcacg atgtcagtcg gcgccggctg gaacggcagg
     1021 tgccgcatcc ggcccagaag tccagccgcc gtcaggtcag aaacgatgac ctccaggcga
     1081 accgcccgct ccggatgcgt ctcacccgtt tgatggcgat ggaagaaccg gtcatagacc
     1141 cagccgacgg tcatgcaagc agcttatccc cgataccgcg ttctgcaaaa ccggacgaat
     1201 cattgttgtc tgatctcctc gcgcggcagg agttgagggg cggtttcgtc gacggtgatc
     1261 aacccggttg cgaattgcag atcgttcatg gttcgctcgg tttcgagcat gctggcggcg
     1321 acctggtcga cctggccgga aatgaaatca ggttcctgcc gattgacggc cagttcgctc
     1381 agcgagcgga ttttgttttc gagccggtcg atttccagtt ggatcagttc gtagttggag
     1441 cgagcctttt gatagttgct caggcggttc cggcaggttt cgagattgtc ctcgatggtt
     1501 cggatgaact tctgtcgctg cgcctcgtct ttcagcctgg ataccttctg caatcgagcc
     1561 tggaggctat cgatgtcctg ctgaatctga gcctcgctgg ttttctggaa gaaccgctca
     1621 agggagtatt gcgtgaacag aaggcgaagg tagatccaca gcaggcggtc caggccggcc
     1681 agttgcgatt gctccaatat cgacgcggag cccgtgtgtc cggggtactt gatggccagg
     1741 gcgatctgtc gcagttcgag gcaacgggct cgaagggcct catatcgttc cagggatttt
     1801 tcgggaaggg ccgcgatgat cgacttcatc atccgttcga cgtcggccgt gccttgctga
     1861 cggagagcgg cgttttgccg ggcatcgaca taagcctgga actttggatg cgttcccagc
     1921 atgcccaggt agccgacctc aacggccagc accagcggca cccagacgtc ggcccggccg
     1981 ctcagcagcg caaagcccat tgcgccggcc agcagcagca ggttccagcg gttgaggaac
     2041 gccgttttga ggtacttgac gaaatacacc ggaccgacct ctagtctcgc ccttcctcca
     2101 gttcgctgat caggtcacga atataggcga ccgtttcctg gaacttcggc tcccgctgca
     2161 tctgcttgga ggggcggtca gcgggctgga ccaccagttc ccgcaggatg gtacccggcg
     2221 agttgctcat gatgtagacg cggtctccga gatacacggc ctcttcgatc gagtgcgtta
     2281 cgaacagcac cgtagcctgc acttccttcc acagcgcgac cagcagatcc tgcatgttca
     2341 tgcgcgtctg cggatcgagg gccccgaacg gctcatccat caggatgatg cggggcttga
     2401 gtatcagcgt gcgggcgatg gccacgcgtt ggcgcatgcc gccggacagc tcgtgcggat
     2461 acttgtccgc gtccgtcttg acgttcaaac cgacgcgctc gatccagtgt ctgcccagtt
     2521 cgtcgcgctc cttgcgaggc ctgtgctgac attccaggcc gaaggtgacg ttctcgagca
     2581 cggtgcggtg atcgaagctc gtgtagtcct ggaagaccat tccgcggtcg gctccggggc
     2641 ccgtcaccgg cctgccgagg acgagcacgc ggccctcggt gggcggatgc tgcggctcga
     2701 ggccggcgac cagccggagg atcgtgctct taccgcagcc gctgggcccc aggacgcaga
     2761 cgaactcgcc tttgtcgggc aggtcttcaa tcacgaagtt gacgtcgcgg atggccgtgt
     2821 agctgttggc ccgacccgcg ttgtaggttt tggtcacgcc ggagaactgg acgacgggcg
     2881 gccgggcaag ctgctcaggc ttgcggatct cgggcaacac ctcgccgata cccaccggct
     2941 cggcgggttt gccgggaccg gcagcggttg tattgtcggt ctcggtcatg gcttcttatc
     3001 cggagtcgag gccggctcgc gggcactgat cgcggcctgg gcaaccgacc tgcggaatat
     3061 gtttatgaaa tcctcataca cgtgcaacac taaccgcaga ccacgattga gaatcccgtg
     3121 actgccgtag cggtgcggga acagctcccg ctgaatccaa aacagcacgc ggtcgatggc
     3181 catcgcgacc agcgggatga tcatcagcac caggagaata tgttctttct ggccccgctt
     3241 ctgggaggtc atgatgatgt cgcccaaccc gccggattcg tcgccgaact tgaccagctc
     3301 ggccagcatg atgtatccga aggccaggcc gaagagcagc cgcagcgagt tgaagatacc
     3361 cggcatggcc agggggacga gtaccttgag gatgacctga cgccgacggg cgcccagggt
     3421 gtaggcggtg tcaatgtacc ggcggtccac gtcgacaacg gcttgagccg aatcggagac
     3481 gatgaacgcc acgcaggcga tgaagatgaa catgatcttc tgctgttcgc cgattccgaa
     3541 gagcgagaag gtcaggggga tcagggcggc gacagggatg ttgcggccga agatcgacaa
     3601 cggcgacagg aaggcgtgga accaagagaa acatccgccc aggatgccca gcggcacgcc
     3661 catgagggtg gccaggccga accccagagc cacccgccgc agactggccc aggtgtttcg
     3721 ggtcaacgcg cgatcgaacc agaggctggg aaaggtggcg aacgtttccg ccgggctggg
     3781 cagcacggtc ggtccgagga gtcgctgctc cggctcgccg cgcgtcgcca gccaccacaa
     3841 accgagaacc atgacaacgc aggcgatgcc gcagagaccg gcctgccagg gcgggatctc
     3901 gcggcgaagg acgaacagtt tgcgcaaggg acaggatcgc tcacagctgg ttgccgtgga
     3961 tgcagcggga gcggggggct ggtgggcgct tgggggcgca acctgtgccg gttgatccgt
     4021 catgcctgct gccgtcctcc atccgaaaag gcagtgtccc tcaccgcttg ttgccaggcg
     4081 gcaggggaca cggtgatcat cgcacaatca ccggctcgcg aacagacccc acatgggcga
     4141 gggctgccga tcgtgggccc cgaagagggg gtcactgtct ttctgccggg tagaccttga
     4201 cctccacgcg gcggttcttg gcatggttgt ccgggtcgtt cgggtcggcc ggcttgtccc
     4261 aacccacgcc ctcgaccgca aactggttgg ggtcaagctt gtacttatta accagggcct
     4321 ccttaaccgc gtttgcccgg ttgagcgaca gctctttcac aaggctcggg gtcacctgcc
     4381 cgcgcatgga ggcgtcggtg tgtccctgga tgataatgcg ggcggccccg aactggccga
     4441 ccagcttggc gatgtcctcg agcacgaaat cgacctgggg atcgtagagc tgctcgacgt
     4501 ccttgccgtc gattttcttg gtcacctttt tgtgcaagtc ccaactgttg gggaagaagt
     4561 gaatgaagac cgtgttggtc aggatggggt cctcggcctt gatctcgcca gcgctggcgg
     4621 gggtgaactg gatccggtac tcgtctttct gggaggcgta cttgggctct ttgccgagct
     4681 tctcgatgat cgagaagtcc atgacctggt cgaacgaaac cggctggtgg gtgatggagc
     4741 cgatgcggcg gtagaggttg taggcctgcc gccagactcg ctcaaagttg gcggggttgt
     4801 tctggttgac gaagaactga taattctccg cccagttggt gctgtgggca tctcccagca
     4861 tgcccagcgc gtcgccggcc gggatgttgt atccgg
//
LOCUS       DAOTPW010000333         4875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14329_length_4875_cov_6.155394, whole genome shotgun sequence.
ACCESSION   DAOTPW010000333 DAOTPW010000000
VERSION     DAOTPW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14329_length_4875_cov_6.155394"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1042
                     /locus_tag="P5316_20250"
     CDS             290..1042
                     /locus_tag="P5316_20250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR87347.1"
                     /translation="MLVLFEERAGEIALLACGEITRRGDSVSNVLPCRGPENMIKILI
                     ADDHTLVRKGIKRMLEEQPDFKVVGEAAEGKETVAKTRETQPDVVLMDVAMPGTDGIE
                     ATKQLTRNRKSKTRVLVLTMYADEHFAARLLRSGASGYVVKDAAPSELVDAIRSVYEG
                     KRFISSSLREALAMRYVNGQAPDPVDAISDREFQVLRLLASGQTNREIAANLGLSVKT
                     VDAHRLNILSKLDLRNNSDLTKFAIRHGIVSS"
     gene            complement(2633..2827)
                     /locus_tag="P5316_20255"
     CDS             complement(2633..2827)
                     /locus_tag="P5316_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87348.1"
                     /translation="MPDRRESRVAPADHVELTPEQVIFPPAGGGGVAGDLLVDVLAAG
                     DVKTQPGIRGSYSAIDVTLR"
     gene            complement(2820..3098)
                     /locus_tag="P5316_20260"
     CDS             complement(2820..3098)
                     /locus_tag="P5316_20260"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRR87349.1"
                     /translation="MGIGYAYILRCNDDRYYYGSTNDLLQRLRCHRCALVKSTRWRLP
                     VRLVWYEQHETLERARQREYSFKNGRTRRKAIEHLIATFPPDKLTPFA"
     gene            3183..3626
                     /locus_tag="P5316_20265"
     CDS             3183..3626
                     /locus_tag="P5316_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87350.1"
                     /translation="MGDFAGSGATWSPYDLDHDHDVDRADFLRLQPCITGPEGPPVLG
                     CEDKDFDHDNDVDQEDFGEFQGCFSGSYMPIAPACMGTALPASGSFTMHGLMIDVLAD
                     GKTLLFARARHYDLKHGRWLQRDPPRFHRRPEFLRGVQRQCDGRR"
     gene            complement(3903..4136)
                     /locus_tag="P5316_20270"
     CDS             complement(3903..4136)
                     /locus_tag="P5316_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HRR87351.1"
                     /translation="MANRKTYHVTPDGDGGWRVKAEGGSRASSTHENKTDAVQSAKDL
                     AKAQPLGQVVIHRTDGQIQTEHTYGKDPHPPKG"
     gene            4308..>4875
                     /locus_tag="P5316_20275"
     CDS             4308..>4875
                     /locus_tag="P5316_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87352.1"
                     /translation="MLFGNRLRRLQRRRNIFWIFAGKLAGEMVYYFTMDARPTTDELR
                     PHGKADEARLRDAGFRWGDKGTHTSRTIMLDELRAVLANCRPDATRDDYLAAIHEDNC
                     LGKRTAATRKLSSQRLSELYALDPDVPLFRVMRRCWYSDRDGQAILAMLLALARDPLL
                     RASAPPVLRMRPGEELARQQMTDALNRVV"
BASE COUNT          956 a         1505 c         1483 g          931 t
ORIGIN      
        1 gccgagggcg ggacgatccg tgttcggcct atgcgaagat aggcatattt cttatccagg
       61 agaaaacgag gacgattgag ccaatcctct aaccaactgt catacccgta ggctcgattc
      121 ctattccccc ctactctgac tcgaatgcaa ccatattctg ccttggtcag accgcttgac
      181 gggcaagcgg agccggacaa tccgacgggt tgtgacgcgt tgaaccggcc ggtcgggcga
      241 ggccaaggca tccggggcct tgatctttga agtctgcgat cgagatgtta tgctagtatt
      301 gtttgaggag cgtgccggtg aaatcgctct tcttgcctgc ggcgaaatca cccggcgcgg
      361 cgactcggtt tccaacgtgc tcccatgcag agggccggag aatatgatta agatcctgat
      421 cgccgacgat cacaccctgg ttcgcaaagg tatcaagaga atgctcgagg agcagcctga
      481 tttcaaggtt gtcggcgagg ccgccgaagg caaggagaca gttgccaaga ctcgcgaaac
      541 gcaaccggat gtcgtgctca tggacgtagc gatgcccggg acagacggca ttgaggccac
      601 caaacagttg acgaggaacc ggaagtcgaa gacgcgtgtc ctggtgctca ccatgtacgc
      661 cgacgaacac tttgcggctc ggttgcttcg cagcggagcc agcggctacg tcgtcaaaga
      721 cgccgcgcca tcggagctcg ttgacgccat ccgcagtgtg tacgagggga aacggtttat
      781 ctcttcgtct ctgcgcgagg cgctggcgat gcgttatgtg aacggacagg cgccggaccc
      841 tgtcgatgcc atctcggacc gcgaattcca ggtcctgcgt ctcctggcgt cgggccagac
      901 gaaccgtgag atcgctgcga atctaggcct gagcgtgaag accgtcgacg cgcatcgcct
      961 caacattctc agcaaactcg atctgcgcaa taactccgac cttaccaagt ttgccattcg
     1021 acacggtatt gtcagcagct aacaaggacc cagcggagtc ggcatgaatt tgcatcccgt
     1081 gtctcctgcg atggtcgacc agcggccatg accgggcgtc agagcgcggc gggaagggag
     1141 cgtctgcctt gcaaagactc ctcgcacagg atccgggaga tccgtgacag aagcggcggc
     1201 agtcctggcg gctgacccgg ttgtggtgca cgcaagccga aagcagaccg cttcaacggt
     1261 ctttcccgaa gggcaaacag gccggtcgtt tgatgactgg ctcacggtcg gctaattcct
     1321 ccttcactgt gttatcccac cgaggccgtt ttaacgggcc tgtccttgtc agttaggccc
     1381 gttgaaacgg gctgagacca tagaaaggaa gaagaaccgc gggcgaccac cagccggcca
     1441 catcacggcc agcctatcag cccacctgtg gcaggccccc cctgaaggag gttaatcgcc
     1501 caacctggac cggtcggcat gtccacccaa actgtcacgc accgggatcc gggagatccg
     1561 gccggcttga cgctttccat gaaggaccgc ctttctgcgg gccgcaaccc aagcgcaccg
     1621 cgatccggaa acctacccac aagccgcaca gacatccggg tgttaggttc cgatgcaagg
     1681 ttatcggccg tttctcgatc gggagcgggc cgtttggcga cgatcggacg cctatccgtc
     1741 tgtccgcctc tgactacctc taataatact tctagcattc cctttcctac acacaagaac
     1801 tggttcttat aagttcgcag gaagcacact tatacattcc tcggcaccgt tgccgaacat
     1861 acaggcgcgg tccgggcaag aacgatcagg cgacggcttt gtcccagcga gtcgggattc
     1921 aggggttcgt cgcggatctc gccggtcttg tctgatgcac ttgtcaatga tcgcgggaga
     1981 cgttgccggc gccgggctgc aggtccggga gccaacagcg ggtcgaacga attgtccccc
     2041 gtttcggtca ggaggataac aggatgagtg ctccggaaac gatagagcgt gtcgagcagg
     2101 gactgctccg gggatgtcca cgtgtccggg ccctagccag tgcgcaccat ctggatggtg
     2161 ttggccgtcc ggcgggcctg cgctggtcat tgtgtttgat gctcttgcct cgatgccaag
     2221 cagcgcgttt gagagcctga ggaggagcgc ctttctgcgg gctggaaccc gagcgcacag
     2281 cgatcgagaa acatgcgcgc aacccgcaca gatgttcagc cataaggttc cggcaacatc
     2341 ttgttgaatc ataacacgtt gtgccccata gttctttcct atcctgccca tctggccgat
     2401 gcttgggttc ctttggcgcg gagagccatc ggcggtggtc aggcaggcga ggggggtgac
     2461 ggctcgtcgg cggacccaga cgtttctgat tgtggtgatg ggttcgtttc gcatttccgg
     2521 ggttctcgtt ccctgtcagt gtggtcacgg cctgttgggg caggccccgc gcagaacgtc
     2581 tcccatgctc ggcaagaagg gataacacgc ggagtgttcc gggacggggg tttcaacgga
     2641 gcgtaacgtc gatggccgag tacgagccgc ggatgcccgg ctgcgttttc acatccccgg
     2701 ctgccagtac gtcgacgagc agatcgccag ctacgccgcc cccaccggcc ggtgggaata
     2761 ttacctgctc gggcgtcagt tcaacgtgat cggccggggc aacccttgac tcgcgacggt
     2821 caggcaaagg gagtcagctt gtcgggcgga aatgtcgcaa tcagatgctc aatcgctttc
     2881 cgacgggtcc gaccgttctt gaatgagtat tccctttgcc tggcccgctc cagcgtctca
     2941 tgctgctcgt accaaaccag ccgtaccggc aggcgccatc tggtggactt caccagggca
     3001 caacgatggc atcgaagacg ctggagcaga tcgttcgtgc tgccgtagta ataacgatcg
     3061 tcattgcatc gcaagatata ggcatagccg atacccatgg gaggtaggat accgcacgaa
     3121 cgctgtcgcg tgtcaagggc aacgccgacg gatcgtcgat caacgggctg aactactcat
     3181 cgatgggcga tttcgcgggc agcggtgcca cgtggtcgcc gtatgatctc gatcacgacc
     3241 acgacgtgga cagggcggac ttcctgcgcc tccagccttg cataaccggc ccggaaggcc
     3301 cgccggtcct gggctgcgag gacaaggact tcgaccacga taacgacgtt gaccaggagg
     3361 acttcggcga gttccagggc tgcttcagtg gttcgtacat gcccatcgcc ccggcgtgca
     3421 tggggaccgc tctgccggcc tccggcagtt tcaccatgca cggcctgatg atcgatgtcc
     3481 tggccgacgg taagaccctc ctcttcgccc gcgcccgcca ctacgacctc aaacacggcc
     3541 gctggctcca acgagacccc cctcggtttc atcgacggcc cgaatttcta cgaggcgttc
     3601 aaaggcaatg cgatggtcga cgctgaccca ttggggcaaa cggcgttgga acgcgaaacg
     3661 ccgacgcgtc tccgcatcgg cgttgtcgtg aacccgtagg ttggtgcatt tcgctatgac
     3721 acgtcaacta gcggggacgc gatcgctacc gcttcacgca atgagggaag ccttctggca
     3781 gaagttccgg ccggcggcgt aaaacgcggc ggataccgag cttgccacga acccgacgaa
     3841 cccgcgacct ccgggccgaa agcccggagg ttgtcttctc agcctcgaac ggccaacgga
     3901 cctcacccct tcggcgggtg cgggtccttg ccgtaggtgt gctcggtctg aatctggcca
     3961 tccgtgcggt ggatgaccac ctggccgagc ggctgagcct tcgccaagtc cttggcgctc
     4021 tgcacggcgt cggtcttgtt ctcgtgcgtg ctcgacgcgc gagaaccacc ttccgccttc
     4081 acccgccatc cgccgtcccc gtcgggggtc acgtggtacg tcttccggtt tgccatgtct
     4141 gaggctcctt atggttcgcc ggctccgacc ccgaacgggg tcagccgagc cggtcgcgac
     4201 gctctcaaca caccagcatg atcgctctgt acgggaccaa aagcaagccg cttccatcct
     4261 cggcaagaga tagcccacga actcctgcac gatagagatt ccataacatg ctgtttggaa
     4321 acaggttacg gcgactacaa agacggcgaa atattttctg gattttcgcc ggcaaactgg
     4381 ccggcgagat ggtatactat ttcaccatgg acgccagacc gacgacagat gaactcagac
     4441 cccacggcaa ggccgacgaa gcacggcttc gcgacgccgg cttccggtgg ggagataagg
     4501 gcacgcacac cagccggacg atcatgctcg acgagcttcg cgccgtgctc gcgaactgcc
     4561 ggcccgacgc gacgcgagac gactacctcg cggcgatcca cgaagacaac tgcctgggga
     4621 agcgcaccgc ggcaacgcgg aagctttcaa gccagcggct ttcggagttg tacgcgctcg
     4681 acccggacgt tccgttgttc cgggtcatgc ggcggtgctg gtactccgac cgcgacgggc
     4741 aagccatcct cgcgatgctg ctggccctcg cacgcgaccc gctgctgcga gccagcgcgc
     4801 cgcccgtcct gcggatgcgg cccggcgaag aactcgcgcg gcagcagatg accgacgcgc
     4861 tcaaccgggt cgtcg
//
LOCUS       DAOTPW010000334         4813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14550_length_4813_cov_5.221732, whole genome shotgun sequence.
ACCESSION   DAOTPW010000334 DAOTPW010000000
VERSION     DAOTPW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14550_length_4813_cov_5.221732"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5316_20280"
     CDS             <1..363
                     /locus_tag="P5316_20280"
                     /inference="COORDINATES: protein motif:HMM:NF036579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HRR87353.1"
                     /translation="TWDAMNDGYQSLNHCMLGHVMEWYYGYVAGIRQQPGSIGWKRIL
                     IAPQPGPLDHAETTFASPAGPITSRWSIRDGKFRLEAEVPEGVAARLIAPDGTEKDVH
                     AGRHKLECAYTWCILAIP"
     gene            634..987
                     /locus_tag="P5316_20285"
     CDS             634..987
                     /locus_tag="P5316_20285"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRR87354.1"
                     /translation="MARLQAYCGLDCSECEAYIATQKNDRAGLEKAAAKWTAEFGAKN
                     LTADMCVCDGCAAGKRASTAHAATCDLRLCASSRKVETCAHCPDYACETLKGFFAFAP
                     ALKDKLEVIRRQLGE"
     gene            complement(1130..3463)
                     /locus_tag="P5316_20290"
     CDS             complement(1130..3463)
                     /locus_tag="P5316_20290"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRR87355.1"
                     /translation="MSNMDATSRCRPLGVLVLVVALLLLLPGPSAPGADLSLEAGKRT
                     FAPGGPAGGGLRFGNPVWSPDGRHLAISDDHGDGVYLYDTQTESVLQITDARSSGYAF
                     NWSPDGRRLGFKLLISKAGSIFPLQAPAVYNLDQQKLVLLCEPTVRAGVPSFSSDGLT
                     AFTVDRELRVVNPAGEIVTSLPLGHYVNLAVISPDGTRVAYNTEDDQVAVLQLSSGKS
                     THLTTGPDAHFKPLWSPDSERLAVSTITARLKCVDLQSRQVHDLDEGTDPSWAPDGQT
                     LFYSRTERLDGAKVIDSDIYQIGCDGSGKRRLTWEVGEHEAAARLSPDGREMAFVSLV
                     TGELYRAPVGRHATTDTQGKERSTYSLSPKVKLNIPAATFVKLDTTRSPLQVHAASEQ
                     PPVITADNPVTLLRTVPYVHQVYDTPDDFNGHWACGASSAIMAINYYSILPYWDITCS
                     WPYSHVSHYGQNVSEIYTYNGVTYDYRAKDASGHWAYGGYAYIVRNNWADTRGYMRDY
                     IINHGLSSAADYSPSWAKLQTEINNDDPFVVLNALTSSGHYITAIGYYSTQHTAVFND
                     PYGDKNTPGYPSYDGAGVLYDWPGYNNGFANLNAVSCFIYCRGGLPPLITEHPVSQTI
                     DWQANTTFSVVAIGEGTLSYRWQKGTTDLVNGSHYSGVTTATLSVLNATDAEAGQYRC
                     VVGNAYGSAASDTATLTVLPRPAPPGDMDGDDDVDQEDFGLLQACVSGASVPQSDPAC
                     TDARLDKDSDVDQADIALFLGCLSGPEVEVDSDCVVP"
     gene            complement(3844..>4813)
                     /locus_tag="P5316_20295"
     CDS             complement(3844..>4813)
                     /locus_tag="P5316_20295"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HRR87356.1"
                     /translation="RDLNRHASRLLSSKPVPAQVAILCDPRVFIQAHGEQDAGLYVAS
                     VRGAYRAFYRASIPADLVISEQLMKADPARYKLICLPFAYLLSREEGQWLSKYVEQGG
                     YLVAGTWCAQKDERTFLYETVPGAGLAELFGCREQAFQTIGQASATLVDGRGLITSLK
                     PGAKLPAQRYTQQLELLPGAEVVAEFDDKSPAMVAARRGKGRTLYAATLLFLGYDATA
                     DGNLQKVLLDAASAAGIEAPIRVQAQPDANEVVARVLEGPGGKTVVIANHSWADKKAD
                     VTIPRAAKARVFDLLSGKDVGTSVDGAAARFSLPIPARQARALAVE"
BASE COUNT          902 a         1522 c         1473 g          916 t
ORIGIN      
        1 acctgggacg cgatgaacga cggctaccag tcgctcaatc actgcatgct cgggcacgtg
       61 atggaatggt actacggcta tgtggctggc atccgccagc agcccggcag catcggctgg
      121 aagcggatcc tgatcgctcc gcagcccggc ccgctcgatc atgccgagac aacctttgcc
      181 agcccggccg gccctatcac cagccggtgg tcaatccgcg acggcaagtt tcgcctcgaa
      241 gccgaagtcc ccgaaggcgt tgccgctcgc ctgattgccc ccgatggcac cgagaaagat
      301 gtccatgccg gccgccacaa actcgaatgt gcctacactt ggtgcatatt agcgattcct
      361 tagagtgtca ggcgaggtag ggcatggtcg gagccgccaa aggcggcgga gacctgccgc
      421 tgggcccgga atccgggcgg gttcctcacc ccgcttggcc cggaatcctt tccgggccac
      481 atgccttcgc ggaatccgtt ccgtgtttgc cgacccagtg gtctcgacac gtctcgcatg
      541 tcgattcccc atacaatggt cgcacatcct ccggccgcgt ggccgggcga agtgatctgg
      601 agaccatcca ccaacaacga tagcggagac aacatggcca gacttcaggc ttattgcgga
      661 ctcgattgca gcgagtgcga ggcctacatc gccacgcaga agaacgaccg ggccggcctt
      721 gaaaaagccg ccgcgaaatg gaccgccgag ttcggcgcga agaatctcac ggccgacatg
      781 tgcgtctgcg acggatgcgc tgcaggaaag cgagccagca ccgcgcacgc ggccacctgc
      841 gacctgcggc tctgcgcctc atcacgcaag gtcgaaacct gcgcacattg ccctgactac
      901 gcctgcgaaa ccctcaaagg cttcttcgcc ttcgcccccg ccctcaagga caagctggag
      961 gtgatccgaa gacaactcgg cgagtagttg cggcaagggg gcctgtcggg gcgtgccctg
     1021 cacgccgatt cgccttgcgg cccggccagt ggcctgcgtt ctcggttcgg ggaagcgacc
     1081 ctgcccgctg agaacggaca atggccacgg tggtccgagt gatcgcatgt cacgggacca
     1141 cgcagtccga atccacctcc acctccggcc ccgataggca cccgaggaac aaagcgatat
     1201 cggcctggtc aacatcactg tccttgtcga ggcgggcatc ggtacatgcc ggatcgctct
     1261 gcggcacgga cgcgccgctg acgcaggcct gcaggaggcc gaaatcctct tgatcgacgt
     1321 cgtcgtcgcc gtccatgtca cctggcggtg ccggccgtgg aagcacggtc agtgttgccg
     1381 tgtcggaggc cgcgcttccg tatgcgttgc cgaccacaca gcgatactga ccggcttccg
     1441 cgtcagtggc gttgagcact gacagggttg cagtcgtgac acccgaatag tgcgacccgt
     1501 tgaccagatc cgtcgttccc ttctgccacc ggtagctcaa cgtgccctct ccgatggcaa
     1561 caacgctgaa ggtcgtgttt gcctgccagt caatggtctg gctcaccggg tgttcggtga
     1621 tcaggggcgg cagtccgcct cggcaataga tgaagcagga gaccgcgttg agattcgcga
     1681 agccgttgtt gtagccgggc cagtcgtaca ggacgcccgc cccgtcatag ctggggtagc
     1741 ccggagtgtt cttgtcgcca tagggatcat tgaagacggc ggtatgctgc gtgctgtaat
     1801 agccgatggc cgtgatgtag tgcccggaag aggtcaaagc attcagcacg acaaacgggt
     1861 catcgttgtt gatctcagtc tgcagcttcg cccaactggg cgaatagtcg gcggcagagg
     1921 acagaccatg attgatgatg taatcacgca tgtatcccct ggtgtccgcc cagttgttgc
     1981 gaacgatata tgcgtaaccg ccgtaggccc aatggccgct ggcatctttg gcccggtagt
     2041 cgtaggtaac gccgttgtag gtgtagattt ccgagacgtt ctggccgtag tggctgacgt
     2101 gcgagtaggg ccacgagcaa gtgatgtccc agtagggcag gatgctgtag tagttgatgg
     2161 ccatgatggc cgatgacgcc ccgcacgccc agtggccgtt gaaatcgtcg ggtgtgtcgt
     2221 agacctggtg aacgtaggga actgttctca gcagggtgac cgggttgtcg gcggtgatca
     2281 ctggcggctg ttccgaagca gcatgaactt gcaggggcga gcgggtcgta tcgagcttga
     2341 cgaaggtcgc cgccggtatg ttcagtttga ctttcggact caatgaatat gtcgagcgtt
     2401 ccttgccctg cgtgtccgtg gtcgcgtgtc ggccaacggg ggcacgatag agctcgccag
     2461 tgaccagtga cacaaacgcc atttcccggc cgtcgggcga caacctcgcg gctgcttcat
     2521 gctcgcccac ctcccaggtc agccgccgtt ttccgctccc gtcgcatccg atttggtaga
     2581 tgtccgaatc gatgaccttg gcgccgtcga gcctctccgt tctggaatag aagagagtct
     2641 gaccatcggg tgcccaggat ggatcggtgc cttcatcgag gtcgtgcacc tgcctcgatt
     2701 gaaggtcaac gcacttcaga cgcgcggtga tcgtgctgac tgccagacgt tctgagtccg
     2761 gtgaccacag gggcttgaaa tgggcatccg gcccggtggt caaatgcgtg cttttgccgc
     2821 tgctcagttg gagcacggca acctggtcat cctcggtgtt atacgccacg cgagtgccgt
     2881 ccggcgaaat caccgccaga ttgacgtagt ggcccagcgg caaagaggtg acgatctcgc
     2941 cggccgggtt gaccacccgc agctcccgat cgacggtgaa agcggtcagg ccgtcgctcg
     3001 aaaacgaagg aacaccggcc ctgaccgttg gctcgcaaag aagaaccagc ttctgctgat
     3061 cgaggttgta gaccgccggt gcttggagcg ggaagatact gcccgccttc gaaatgagca
     3121 gcttgaatcc cagccgcctt ccatcgggtg accagttaaa cgcatacccg ctgctccggg
     3181 catccgtgat ctgaagaacg ctctctgtct gggtatcgta caggtaaaca ccgtcgccgt
     3241 gatcgtcgga gatcgccaga tgacggccgt cgggtgacca gacgggattg ccgaaacgca
     3301 atccgccgcc cgcgggtccc ccgggggcga atgtcctctt ccccgcttcg agtgaaagat
     3361 ccgctccggg cgccgacggg cccggcagca ggagcaacag agccaccacg aggacaagca
     3421 caccgagcgg gcgacatcgg cttgttgcgt ccatgttact catgttgatc ctttaccttg
     3481 gccagactct aaccgatctt gaaacctgcg cggagagatg attgaaaggt cctgccacgg
     3541 gaccattccc ctccacggcc gacaggtcca tcttaactgc aacccgtctt ctctgggatg
     3601 cttcttgtcg cgttcaggcc attttcgagc cggcggcttc gcttagcgcc cggtcacccg
     3661 gggacgcgga ccgagcatgc tcttcgaacg tatcgtcttg tgctgcaagg ggatagaaaa
     3721 ggcctgcggg caccacgcct cgtgcttgtt ccaggcaggg accggtaacc aggttccccg
     3781 cgccgcgttc aacactctcg ccctccaagc cctgcaacac gcggcaccgg ggaccgacat
     3841 ctcttactcc acggccagcg cgcgggcttg ccgagctgga atgggcagcg agaatctggc
     3901 agcagcccca tcgaccgacg ttccgacgtc tttcccactg agcaggtcaa acaccctggc
     3961 ctttgccgcg cggggtatcg tcacgtcagc cttcttgtcg gcccaactgt ggttcgcgat
     4021 cacgacggtc ttgcccccgg gcccttcgag cacgcgggcg acaacctcat tggcgtcggg
     4081 ctgcgcctga acacgaatcg gtgcctcaat gccggcggcc gacgcagcat cgagaagcac
     4141 cttctgcaga ttcccgtccg cggttgcgtc atatccaagg aacagcagcg tcgcggcata
     4201 cagcgtgcgg cccttacccc ggcgggcggc gaccatggcc gggctcttgt cgtcgaactc
     4261 ggcgaccacc tccgcgccgg gcaacaactc gagttgctgc gtatagcgct gggcgggcag
     4321 cttggcaccg ggtttgagcg aggtgatcag gccgcggccg tcgacaagcg ttgccgatgc
     4381 ctggccgatc gtctgaaatg cctgctcgcg gcagccgaac agctccgcga gccctgctcc
     4441 cggcacggtt tcatacaaga acgtccgctc gtccttctgt gcacaccacg tgcccgccac
     4501 gaggtatccg ccttgctcga cgtatttcga cagccattgt ccttcctcac gagacagcag
     4561 gtacgcgaac ggcagacaga tcagcttgta tcgggccgga tccgccttca tgagctgttc
     4621 cgaaatcacc aggtcggccg gaatgctcgc tcggtaaaag gctcgataag ctccacgtac
     4681 gctggcgaca tatagccccg catcctgttc gccgtgcgcc tggatgaaaa cccgcggatc
     4741 gcaaaggatt gcgacctggg ccggcaccgg cttgctcgag agcagtcggc tcgcatggcg
     4801 attcagatct ctt
//
LOCUS       DAOTPW010000335         4809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14563_length_4809_cov_4.462768, whole genome shotgun sequence.
ACCESSION   DAOTPW010000335 DAOTPW010000000
VERSION     DAOTPW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14563_length_4809_cov_4.462768"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="P5316_20300"
     CDS             complement(<1..658)
                     /locus_tag="P5316_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87357.1"
                     /translation="MGLINGALQIGKTALLAYQSALQVTGNNVANAGSSTYTRQTPVL
                     QPVIGGAIPEGFMPGGGVALADLRRNIDESLMNRLRIATSQMAMVTVQQQNIGRIEST
                     MNELSEVDLSTLLQAFFNSFSALQNEPQSLAQRDIVLSAATSLISALRQQRGDVLALR
                     DELNNQIQRDTERAAKLVENIRDLNVRIVAMEGGGQFGANALRDQRDQYLAELAEIIQ
                     V"
     gene            complement(662..1183)
                     /locus_tag="P5316_20305"
     CDS             complement(662..1183)
                     /locus_tag="P5316_20305"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HRR87358.1"
                     /translation="MTQVKADNLAKELVRLLNDMAGIHEELTVLMRSKLEAIRRADGD
                     AIQSVTVRETALANRLAEREGLRRDLVRNILRELGMSSRKPQNIRMTELAECFAEPRR
                     SRILVATTGLRARLEQIERLSATTRMITTEMLRHMEEIIGVMTSGGPTTGVYSRAGSR
                     ETSGRASVFEAVG"
     gene            complement(1186..1488)
                     /locus_tag="P5316_20310"
     CDS             complement(1186..1488)
                     /locus_tag="P5316_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87359.1"
                     /translation="MSDLRVNSVLQSPPQATAAVNKGRPALRKAVGEVVGTVFYGPML
                     RIARNSRIEGKYGHGGRGEKVFQAQLDQEFLSRAGGRMRNSLTEAICSRLAGGKEG"
     gene            complement(1532..2743)
                     /locus_tag="P5316_20315"
     CDS             complement(1532..2743)
                     /locus_tag="P5316_20315"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRR87360.1"
                     /translation="MRRVDARSRESVMKGEKRVFRLLATALIVGLAVPAAMAVKIGDI
                     THLQGSRVNRLVGYGLVVGLNGTGDGGSYMPAMQALAKAHERFANPVVTLQELKNVKN
                     VAIVLVEASLPRDGAREGERIDVQVSSIGAAKSLLGGRLLVTPLVGPHPQDTRGAMAL
                     ASGPLHVEDPALPTVAKIVQGATLEQDWIHNYVALGRELAIARNRDAGQSLAWLRPDE
                     PYVTLVIDESVAGWGVANTIAQAINADVAISAGAADDPAAQIAVACDPRTVAVRLPEA
                     ERNDPAAFLARLEILDLFMQFAEARVTINRRTGTIVMTGDVEIAPAVISHKGLTVETL
                     VPEPAPTPENPRVVRQEFVGLDPGHKGGAKLADLLDALNRLQVPAADKIAIIDQLKKT
                     GKLYATVVEEQ"
     gene            complement(2775..3518)
                     /locus_tag="P5316_20320"
     CDS             complement(2775..3518)
                     /locus_tag="P5316_20320"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRR87361.1"
                     /translation="MSRMTPFVLGVCLLASQAASQSSSLWRVPPADHRLQAETSAQGP
                     APGGFVAAPLREDVSPATRSIEAVSLIAIPRTQPRKFRPHDLVTIIVKQKKSYESEAK
                     MDTERKWDINGKLSEWFRFHEDHKLGTDSLRFGQPGFKFKFNNKYENDGENGREDKFE
                     TRIQATVVDVKPNGNLVLEAHQQETHDDEIIDLTLTGVCRSEDVTPANTILSTQVAEM
                     VLVEKNSGAMRDATQRGWIPRLLDWAKPF"
     gene            complement(3518..4648)
                     /gene="flgA"
                     /locus_tag="P5316_20325"
     CDS             complement(3518..4648)
                     /gene="flgA"
                     /locus_tag="P5316_20325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRR87362.1"
                     /translation="MILPTQARGTTPASQPTTERSHPNQEITARLHDTAIVNDDQVTL
                     GDIAEIHGDLVGLAASCPIILSPKPGYSLSLTIDDVQKALADRGANLAHWTFRGSSRC
                     TVTRPIRRENQTCPSSKRSGDRSAGPKTVPEVPDRTTTETTPGAAAVSPDTLGGAMHR
                     FIRQRLAELGGTPVIQFPKTLSRQLELSKPTYEFRVDSRSNELLGLVPLDVTIIDPDG
                     QEQALQVSAKVSLRKAVLVAGRPINRGAVVAASDITLQEQIFDRVEDVGLARPAAIIG
                     QRARRFINKGDKLLARDFEPLPLVYRNDLVTVLIDRGNLRIKGTARALGSGAFGDVID
                     VRNEMGERSAGRFSAVVVGAKTVEVTGEDRPTTTAPADRGAM"
BASE COUNT          804 a         1539 c         1465 g         1001 t
ORIGIN      
        1 ggacctggat gatttcggcg agctcggcaa gatactggtc ccgctggtcc ctgagggcgt
       61 tggctccgaa ctgaccgccc ccttccatgg ccacgattcg cacattcagg tcccggatgt
      121 tctcgacgag cttggcggcc ctctcggtgt cgcgctgaat ctggttattg agttcgtccc
      181 tcaaggccag gacatcgccc cgctgctgcc gaagggccga gatcagggag gtggcggcgc
      241 tcaggacgat gtcccgctgc gccaagctct gcggctcgtt ctggagggcg ctgaaggaat
      301 tgaaaaacgc ctgaagcagc gtggagaggt cgacctcgct gagttcgttc attgtgcttt
      361 cgatgcgtcc gatgttctgc tgctgcaccg tgaccatagc catctggctg gttgcgatgc
      421 gcagccggtt catgagggac tcatcgatgt tgcgtcgcaa gtccgcaagg gcgacgccgc
      481 caccgggcat aaagccttcg ggaatggcgc cgccgatcac cggctgaagc acgggcgtct
      541 ggcgcgtata tgtggagctg ccggcgttgg cgacgttatt gcccgtcacc tgcagtgcgc
      601 tttgataagc cagaagcgcc gtcttgccga tctgtaatgc gccgttaatc aagcccatga
      661 ttcatcccac cgcctcgaac acgcttgccc gaccggaggt ctcgcggctt cccgccctcg
      721 agtacacgcc cgtcgtcggc ccgcctgagg tcattacccc aatgatttct tccatatgac
      781 ggagcatttc cgtggtaatc atcctggtcg tggcactgag ccgttcgatc tgctcgagcc
      841 ttgcccgcaa ccccgtggtc gcgacgagga tccggctgcg tcgcggctcg gcaaagcact
      901 ccgcgagttc ggtcattctg atgttctgcg gttttcggga actcattccg agctctcgaa
      961 gaatgttgcg gacgaggtcg cggcgaagcc cctcgcgttc ggccagcctg ttggcaagag
     1021 ccgtttcgcg aacggtgacg gattgaatcg catcgccgtc cgcccggcgt attgcctcca
     1081 gtttgctgcg catcagcacc gtcagctcct cgtggatgcc cgccatgtcg ttcaggaggc
     1141 gaacgagctc ttttgccagg ttgtccgctt tcacctgcgt cactgtcaac cttcctttcc
     1201 cccggccaga cggctgcaaa tcgcctcggt caggctgtta cgcatgcggc cgcccgcacg
     1261 actcaggaac tcctgatcga gttgagcctg gaagaccttc tccccgcgtc ccccgtgccc
     1321 gtatttcccc tcgatcctgc tattgcgtgc gatccgcagc ataggaccat agaacaccgt
     1381 accgaccacc tcgcccacag cctttcgcaa tgccgggcgg cctttgttga ccgcagccgt
     1441 tgcttgtggc gggctctgaa ggacggaatt cactcgtaaa tcgctcattc gttcggctcc
     1501 tcgcggtccg gccgggtttc atgtccaccg gctactgctc ctcaacgacc gtggcgtaca
     1561 acttgcccgt cttcttcaat tgatcgatga tcgcgatctt gtcggcggcc ggcacctgaa
     1621 gccggttcag tgcgtcgagc aagtccgcga gcttcgcgcc gcccttgtgc ccggggtcaa
     1681 gaccgacaaa ctcctgcctg accactctcg gattctcagg cgtcggagca ggttcgggta
     1741 ccaacgtctc gaccgtcagg cctttgtgcg aaatgacggc cggcgcgatt tccacgtcgc
     1801 cggtcatgac gatggttccg gttctccggt tgatcgtgac gcgggcctcg gcgaactgca
     1861 tgaagagatc gaggatttcc aggcgtgcca gaaacgcggc cgggtcgttt cgttccgctt
     1921 ccggaaggcg aaccgcgacg gttcgcggat cgcaggccac ggcgatctgg gcggcgggat
     1981 cgtcggccgc accggcgctg atcgccacgt ccgcattgat cgcctgggca atggtgttgg
     2041 cgacgcccca tccggcgaca ctctcatcga tgaccagcgt gacgtagggc tcatcgggac
     2101 ggagccaggc caggctctga ccggcatcac gatttcgggc gattgcgagt tctcttccca
     2161 gggcaacgta gttgtggatc cagtcctgtt cgagcgtggc gccctgcacg attttcgcga
     2221 cggtcggaag ggcgggatct tcgacatgca gcggtccgga cgccaaggcc attgccccgc
     2281 gcgtgtcctg cggatgggga ccgaccaagg gggtaaccag caggcggccg ccgagcaggc
     2341 tcttggccgc cccgatgcta ctcacctgca cgtcgatgcg ctccccttcg cgggctccgt
     2401 cgcgcggtag agaagcctcg accagaacaa tggccacgtt tttgacattc ttcagttcct
     2461 gcagagtgac caccggattg gcgaatcgct cgtgggcctt ggccagggcc tgcatcgctg
     2521 gcatgtagct accgccgtcg ccagtgccgt tcaggccgac caccagcccg tagcctacca
     2581 agcggttgac ccgcgagccc tgcaaatgcg tgatatcgcc gatcttgacc gccatggcgg
     2641 cgggcacagc cagacccaca atcagggccg ttgccagaag ccggaatacg cgtttctcgc
     2701 ccttcatcac tgactctctc gatctcgcat ccacccgccg cattcagatt ctcccgccgg
     2761 attcttccgc ccggtcagaa gggctttgcc cagtcaagca gccgggggat ccaacctctt
     2821 tgagtcgcgt cacgcattgc gccgctgttc ttttcgacga gaaccatttc cgcgacctga
     2881 gtgctgagaa tggtgttcgc cggggttacg tcttcgctgc ggcagacgcc ggtcagcgtc
     2941 agatcgatga tctcgtcgtc gtgggtttcc tgttgatggg cctccagcac gaggttgccg
     3001 ttgggcttca cgtccacgac cgtggcctgg atccgggtct cgaacttgtc ttctcgcccg
     3061 ttctcgccgt cattctcgta cttgttgttg aatttgaact tgaagccggg ctgaccgaat
     3121 cggaggctgt ccgtgccgag tttgtggtcc tcgtggaaac ggaaccattc gctcagcttt
     3181 ccgttgatgt cccactttct ctcggtgtcc atcttggctt ccgactcgta tgactttttc
     3241 tgcttgacga tgatggtgac cagatcgtga ggtcggaact tgcggggctg cgtacgggga
     3301 atggcaatca gcgaaaccgc ctcgatcgac cgcgtagccg ggctgacgtc ctccctgaga
     3361 ggagccgcga cgaagcctcc gggcgcaggg ccctgggccg aggtttccgc ctgcaggcga
     3421 tgatccgccg gcgggacccg ccacagggac gaactctgcg aggccgcctg ggaggccaac
     3481 aagcaaactc cgagaacgaa cggcgtcatt cgtgacatta catcgcccct ctatccgccg
     3541 gcgcggtcgt ggtcggtcga tcctcacctg tgacttcaac cgtcttggcg ccgacgacga
     3601 ccgccgagaa ccgtcctgcg gatcgctcac ccatctcatt gcgaacgtca atcacgtctc
     3661 caaatgcgcc ggagcccagc gctctggcgg tccccttgat gcgcaggttt ccgcggtcga
     3721 taagaacggt gacaagatca ttgcggtaga caaggggcag cggttcgaaa tccctggcta
     3781 gaagcttgtc ccccttgttg atgaagcgtc tggctcgttg cccgatgatc gcggccgggc
     3841 gggccaaacc gacatcctcg acgcgatcga agatctgctc ctgcaaggtg atgtcggagg
     3901 ctgcaaccac ggcgcctctg ttgatcggcc ggccggcaac gagcacggcc ttgcgcagtg
     3961 agaccttcgc ggagacctgc agtgcctgtt cctgcccgtc cggatcgatg atggtcacat
     4021 cgagcggcac aagaccgagc agctcattgc ttcgggagtc aacgcgaaac tcatatgtgg
     4081 gtttggacaa ctccaactga cgagaaagtg ttttggggaa ctggatcaca ggcgtgccgc
     4141 ccaattcagc caagcgctgt cgaatgaacc gatgcatcgc cccgcccaag gtgtccgggc
     4201 tgaccgcggc tgcaccgggg gtcgtctcgg tcgtcgttcg gtccggcact tccggcacgg
     4261 ttttcgggcc agcggagcga tcgccggacc gcttagacga cggacacgtc tggttctctc
     4321 gacgaatcgg cctggtaacg gtacaccgac tgcttccgcg gaaggtccag tgtgcgaggt
     4381 tcgcgccgcg gtcggccaag gccttctgaa catcatcaat tgtcagggag agggaataac
     4441 ccggcttcgg ggaaaggatg atcgggcaag acgcggccag gccgacaagg tctccgtgga
     4501 tttcagcgat gtcgcccagg gtgacctggt cgtcgttgac gatggccgtg tcgtgcagcc
     4561 gggcggttat ttcctgattg gggtgggaac gctcggtcgt cggctgcgac gcgggagtcg
     4621 ttccccgtgc ctgggtcggc agaatcactg ccaggccaca ggccgcagcg catcgaatca
     4681 gccaccggcc ccggcgatca cttcgtcgct ttgtgccgca ccagattgtc cttgctgcat
     4741 cctgcatcga cgacctccgc gtcgcaaggg tctcccgcgg gcagctcact ccctgtaccc
     4801 gctaccgac
//
LOCUS       DAOTPW010000336         4786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14635_length_4786_cov_5.294018, whole genome shotgun sequence.
ACCESSION   DAOTPW010000336 DAOTPW010000000
VERSION     DAOTPW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14635_length_4786_cov_5.294018"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="P5316_20330"
     CDS             <1..672
                     /locus_tag="P5316_20330"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRR87363.1"
                     /translation="LHFWQDGQWRSMAATYKGEELKINDMIADPQGRIYAGTMYWGAG
                     GMEKTGCLYLIAPDASVRVVDEGIELANGLGFSPDNRTLYFTDSTARKIYAYDVNPST
                     GELSGRRVVVQVPTDEGIPDGLTVDAEGYIWSAQWYGSQIVRYDPQGKVERRIAMPVK
                     QVSSIIFGGDDLTDLYVTTAGQSWVGPYAPPGYDFNAPNIGGSLYRIRVGIQGRPEHL
                     ADFAW"
     gene            765..3272
                     /locus_tag="P5316_20335"
     CDS             765..3272
                     /locus_tag="P5316_20335"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRR87364.1"
                     /translation="MPMMSLPKGGDVAVTSVVLILNAITLMGAAVERHLDLPTSLASR
                     PAVGVADISATLDAAAKPLLPGVRQSLAVKPVPWLVKTHDVGSPTDFHKDIPWHLLDK
                     DKDARFDLPHRGKLGVFNTNSDVHLLIGLGVDDKPVNFNPKRQYTDHLLNLASVKVDL
                     DDILGQVLFLAAESDTLLSVVQVQNLGPHPHIVRVEAACTKPRAEKVPFDRFGYGVTA
                     TTGDLQWIGYNEPNGALVSSYDEWIPGPRRLGGTLLCVLAASEPPAGRRTALTDHATP
                     SGIPRDAVLEHRVELPAGASRTLLLSLNLHRYGPQQYESPMEMILYPKQTPEEALQYS
                     VFAAIRTLSVDWPRLVADSFKWYEHVPLLTVPPQSWAADFLCALELPRANTWSPQEQS
                     SLPWYTLCRPHTNEPYGWWSYGMHGHEDLSTFVVNLTEPTLSQSFLRGHFRNQQPDGR
                     FPYGVNHRGVSAHSGDLATAPFLAWESWTTYLWRDDRKFLQEAYDCCARSLRWWCSPA
                     RTRPGMRLQHWKDFLETVRDDGDLATWTATDKAENQEALDLNYYLLKEERTLAEMARE
                     LGRSGESSQWKADAEQRAAVMREHFWHAEDGVYYGRDIAGDRWARIMDISTFFPLWCG
                     LATPQQVPAIVDLLRDPTAFGANYPVATLAVRHMPDRLKGQWHWRGANWVEMTWLVIC
                     GLKEVGRFDEAARLAEINCRMVFQSLEKDGHFREYYNSLTGEPADLTDYIWTSIPAIM
                     IVDVILGIRPTAEGVEIMPALPESWPDAAIDSLRLRASRLSVSVRRDTAGQHTRAALN
                     GRALPVREGRGILVQWSDLPADARIEITQPSRVAAVR"
     gene            complement(3306..>4786)
                     /locus_tag="P5316_20340"
     CDS             complement(3306..>4786)
                     /locus_tag="P5316_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87365.1"
                     /translation="YIFKPAKSLHFQNDAGTCPPPDGHHYASTFSVPPAPGAQPESWA
                     YQTIQVDNTKTYTVSGYFAGAGDNTVILDLLDGNEQAEHVPDSVSGLIHDNGPAYDWT
                     FAYAQGKPTGDLLTVRWRVTTRNPGPHVAHADALRMDTCTSAITAATIAPADGDNTGT
                     LTGVEIVGSGFSGENTPKVMLTRTGLVAVNATNVTVHDDTRLTCDFNLTDQPTGPRDV
                     IIFKEGCVARIKDGFLILAPALANGGFELPDPGTPVDCTTPEALLRGVPRGWSVKLTV
                     PGSLDRDHHVHRPVTCPSTAGGHYGSLTIDRSGDLIAYQSVRVIPTARYTLAGQFAGG
                     GTNQAVMQLIDGSLESGTLLAETAINDDAFGTSYDWRSRSVSAHAASEVLTVAWRLST
                     SGQDLHALHADGLTFAMTGNPCSRPFADADADGDVDQADFAVWQTCFTGSGGTIPADP
                     PYCRCFDRDSPNGDGDIDLQDYVLFEACASGAGIPASASCAD"
BASE COUNT          911 a         1447 c         1502 g          926 t
ORIGIN      
        1 ttgcacttct ggcaggacgg tcagtggcgc agcatggcag caacgtacaa aggcgaagaa
       61 ctgaagatca atgatatgat cgccgatccg cagggccgca tctatgccgg caccatgtat
      121 tggggcgccg gcggcatgga gaagacaggt tgtctgtatc tgatcgcgcc cgacgcatca
      181 gtccgcgtgg tcgatgaggg catcgagctc gccaacggcc tgggtttcag tcccgacaac
      241 aggacgctct acttcaccga ctccacggcc cggaagatct acgcttatga cgtcaatccc
      301 tcgacgggcg agctgtccgg ccgtcgggtt gtcgtccaag tgcctaccga cgagggcata
      361 ccggacggcc tgaccgtcga cgccgagggc tacatctgga gcgctcagtg gtacggttca
      421 cagatcgttc gatatgaccc ccagggcaag gtggagcggc gtattgccat gccggtcaag
      481 caggtgtcaa gcatcatctt cggcggtgac gatctgaccg atctgtacgt cacgaccgcc
      541 ggccagtcct gggtcggccc ctatgctccc ccaggatacg acttcaacgc tcccaacatc
      601 ggcggttcgc tctatcggat ccgcgtcggt attcaaggcc ggccggagca tttggccgac
      661 tttgcgtggt gaaggaggcc ccaagagggg caacgggcag cacaaaaggt ggtctgtcgc
      721 ggcatcgtct gatttcctcc gcccggtttc ctgctggaga ggcaatgccg atgatgagct
      781 tgcccaaagg tggcgacgtg gctgtgacat ctgttgtttt gatcctgaac gccattaccc
      841 tcatgggggc ggccgtcgag cggcatcttg atttgccgac aagccttgcg tcgcggcctg
      901 ctgtgggcgt cgctgacata tcagcaacgc tcgacgctgc ggccaagcca ctcctcccag
      961 gtgtccgtca gagcctggct gtcaaaccgg tgccgtggct ggtcaagact cacgatgtgg
     1021 gctctcccac cgacttccac aaggacatac cctggcactt gcttgataag gataaggatg
     1081 cccgttttga tctcccgcat cggggcaaat tgggcgtttt caacaccaac agcgacgttc
     1141 atctcctgat cgggctgggc gtggatgata agccggtcaa cttcaacccc aagcggcagt
     1201 acaccgacca tttgcttaac ttggcgtccg taaaggttga ccttgacgac atccttggac
     1261 aggttctgtt cctggccgcg gaaagcgata cgttgctgtc cgtggtacag gtacaaaatc
     1321 tcggcccgca ccctcacatt gttcgcgtcg aggctgcttg cacaaaacct cgcgcggaga
     1381 aggtgccttt cgaccgcttc ggctacggcg tgaccgccac gaccggcgac ttgcagtgga
     1441 tcggttataa cgaaccgaac ggagcacttg tttcgagcta cgatgagtgg ataccgggcc
     1501 ccaggcggct cggcggcacc ctgttatgtg tgctggcggc gagtgagccg ccggccggcc
     1561 gtcgcaccgc cctgacagat cacgcgaccc catcaggtat tccccgtgac gccgtgctcg
     1621 aacatcgcgt cgaattgcct gcaggcgcca gcagaacgct gctgctcagc ctgaatcttc
     1681 accgctacgg cccgcagcag tatgaatcgc cgatggagat gattctctat cccaagcaga
     1741 cgccggaaga ggctctccaa tactcggtgt tcgcagcgat ccggacactc tcggtcgatt
     1801 ggccacggct cgtggccgat agcttcaagt ggtacgagca cgtgccactg ttgaccgtac
     1861 caccccagtc ctgggcggcc gacttcctgt gcgctttgga gctgccgcgg gccaatacct
     1921 ggagtccgca ggagcagtcg agcctcccgt ggtacacgct ttgccgtccg cataccaacg
     1981 agccttacgg ctggtggagc tacggcatgc acggtcacga ggatctctcg actttcgtcg
     2041 tcaaccttac cgagccgacg ctgtcccaaa gtttcctccg cggacacttc cggaatcagc
     2101 agccggatgg acggtttcct tacggggtga accaccgcgg agtcagcgcc cacagcggcg
     2161 acctggccac tgctcctttc ctggcatggg agtcttggac gacgtacctg tggagggacg
     2221 atcggaagtt cctgcaagag gcttatgatt gctgcgcacg ctcgcttcga tggtggtgct
     2281 cgccggcccg cacccgcccg ggcatgagac tgcagcactg gaaggatttc ctggagaccg
     2341 ttcgcgacga cggcgacctg gcgacgtgga ccgccaccga caaagccgag aaccaggagg
     2401 cactggacct caactattac ctgctgaaag aggaacgaac gctggctgaa atggcccgcg
     2461 aattgggccg cagtggcgag tcgtcgcagt ggaaagcgga cgcggagcaa cgggcggcgg
     2521 tcatgcggga acacttctgg catgccgagg acggtgttta ctacgggcgc gatatagcgg
     2581 gtgatcgttg ggccaggatc atggatatct cgacgttctt tccgctgtgg tgcgggctgg
     2641 caacgcctca gcaggtgcct gcgatcgtcg atctgctgcg agaccccact gcgttcggcg
     2701 ccaattaccc cgtggccacg ctggccgtca ggcacatgcc ggaccgactt aagggacagt
     2761 ggcattggcg cggagccaac tgggtggaaa tgacgtggct ggtgatctgt ggtctcaagg
     2821 aagtgggtcg atttgacgag gccgcacgcc tggctgaaat caattgtcgg atggtttttc
     2881 aatcgctcga gaaggacggc catttccgcg agtactacaa ctcgctgacc ggtgagcctg
     2941 ccgatctgac cgactacatc tggacgtcaa tacccgccat catgatcgtc gacgtgatac
     3001 ttggtatccg gccgacggcc gaaggcgtgg agatcatgcc ggccctgccg gaaagctggc
     3061 ctgacgcagc gattgactct ctgcgacttc gcgcgagcag gttgtcggtg agcgtgcgac
     3121 gcgacacggc cgggcagcat acccgggccg ccttgaacgg ccgggctctg cccgtcaggg
     3181 agggacgagg aatcctcgtc caatggagcg acttgcccgc ggatgcgcgg atcgagatca
     3241 ctcaaccctc gcgcgtggcg gctgtacgct gaaggatggt tcgagttcgt ccagacgacc
     3301 gccgttcagt cggcgcacga agcgcttgcc gggatgcccg ctccgctggc acaggcctca
     3361 aaaagaacgt agtcctgcaa gtcgatgtca ccgtcgccgt tagggctgtc gcggtcgaaa
     3421 caacgacagt aaggcggatc ggcggggatt gttccgccgc ttccggtaaa gcacgtctgc
     3481 cagacggcga aatcagcctg gtccacgtct ccatcggcat cggcatctgc aaacgggcgg
     3541 ctgcacggat taccggtcat ggcaaacgtg aggccgtccg cgtggagcgc atggagatcc
     3601 tgacccgacg tgctcagcct ccaggcgacc gtcaggacct cactcgccgc gtgtgccgat
     3661 acggagcggc tgcgccagtc gtaggaggtc ccgaaggcat catcattgat ggcggtctcg
     3721 gcaagtaacg ttcctgattc cagcgagccg tcgatgagct gcatgacggc ctgattggta
     3781 ccgccaccgg cgaactgccc cgcaagggtg taccgagcgg tcggaatcac gcgcaccgac
     3841 tggtaagcaa tcagatcacc actccgatcg atggtgagtg agccgtagtg tccgcctgcc
     3901 gtcgacggac aggtcacggg tcggtgaacg tggtggtcgc ggtcaaggct gccgggaacc
     3961 gtcagcttga ccgaccagcc tcgcggaaca cctctcagca acgcctcggg agtcgtgcag
     4021 tcaacgggtg tgcccgggtc aggtaattca aaacctccgt tggccagcgc cggcgccagg
     4081 atgaggaacc catccttgat tcgagctacg catccctcct tgaaaataat gacatctcgt
     4141 ggtccggtcg gctgatcggt gagattgaaa tcgcaggtca gccgtgtgtc gtcgtgaacc
     4201 gtcacattcg tcgcattgac cgccaccagg cccgtacgcg tgagcataac cttgggggtg
     4261 ttttcgccgc tgaaccccga gccgacgatc tcaacgccgg tcaacgtccc ggtgttgtca
     4321 ccgtccgccg gagcaattgt ggcggcggtg atcgccgatg tacaggtatc catgcgcagc
     4381 gcgtccgcat gagccacatg aggcccgggg ttccgcgtcg ttacccgcca acgaacggtc
     4441 aataggtcgc cggtcggctt gccctgcgcg taggcgaagg tccagtcgta ggcaggaccg
     4501 ttgtcgtgga tcagaccgct gaccgagtcg ggaacatgtt cggcctgctc gttgccgtcc
     4561 agaagatcca ggatcactgt gttgtcgccg gcccccgcga aataccccga aaccgtgtag
     4621 gtcttggtgt tgtccacctg gatggtctgg tacgcccagg attccggctg cgctcccgga
     4681 gccgggggta ccgagaaggt gctcgcataa tgatggccgt cgggaggtgg gcaagtgcca
     4741 gcgtcgttct gaaagtgcag agatttcgcc ggtttgaata tgtagt
//
LOCUS       DAOTPW010000337         4775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14681_length_4775_cov_6.058898, whole genome shotgun sequence.
ACCESSION   DAOTPW010000337 DAOTPW010000000
VERSION     DAOTPW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14681_length_4775_cov_6.058898"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="P5316_20345"
     CDS             complement(<1..1541)
                     /locus_tag="P5316_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87366.1"
                     /translation="MFDKNGPCGSLTADLARTIIYNTATRQDWAYPWCQLPDQPKHLN
                     ALTAVNAVTAGSCGPVVGDLNCDSILDGSDLDGFSVCMTGPRTDPIGGGCTCADFPAS
                     PPDGDVDLHDFMYFQRLFGGGQSTAATVTLDLTSPNAGQVVEPGGTVYWTVSATVSQG
                     DNAGLAAISVDLVQDPPSGRLVDMSPATTVGTGMDGFVRPAGISNPETDVPGAGYGGT
                     PVARNLRQIGGAQNTLGEAGDTGTGEDVIVDPGIGQSGPQIIAQGSFTIPLVAGTYVF
                     RIENGLANVLDVVSPPPSPPAAWPVSAANVVLNSPSFSVIVPYDLAVPDFDRDGDVDQ
                     NDFALMQACYTGNTYTIAQLFPGGDCDAFDLDGDGHVKMRDPGNPTETLGDLDWLEKC
                     ASGPGIRADSDCCDCDGHGYLDARDNPPDPWVNNFDYDGDGIQVLGRPNCTGGNTLDC
                     DDNCPCQENSDQADDDADGIGDVCDPTPYNAPSPDPLDPCETPENHFTITAWRSVRTH
                     GAAGS"
     gene            complement(1541..2377)
                     /locus_tag="P5316_20350"
     CDS             complement(1541..2377)
                     /locus_tag="P5316_20350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HRR87367.1"
                     /translation="MQSRILVTAAVSVLTSAAFGMHESRSFINADDVHAQGMSGHGVT
                     VAVIDTGIDYTHPGLSGSIAAGGLSIEGGVIQPDMGEDVYSLYHGTYMSLIITDPWGV
                     APGAKILSIRVHGPSGGANPTDVMLGILYAMNRSLTADPSIRVINRSGGMGAYQCSCD
                     AWWPAYNQVISDAFANGIITFAATGNEAVCGGISMPACFSSAVPVAANYDADWYPGQY
                     FEPPADCADLNPERYRVVCFSNATDNCGHFLAAPGFSITVGDSPETARRRLPPTAQVW
                     RR"
     gene            complement(3233..>4775)
                     /locus_tag="P5316_20355"
     CDS             complement(3233..>4775)
                     /locus_tag="P5316_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87368.1"
                     /translation="HRIIKPADLQHVQTGADIDTQSGSCIWDFAEYDSDYDTGHLYAG
                     AWAGNGGPVYRTEPDFLCDQLRSSLPRVDFTVNYAQNNNNRGVNVWALETFGGTAIPK
                     AKLYAGGGVDRNGARLWSFDGHEWSLVFAHPDSTTNNEGVSALVAHQGRLFVGLGLPD
                     GYYAGDGIAEVWVSTDGILFEIASLPDQFGGGVQCFLSAGTSGDCNGNGVPDECDIAN
                     STSEDCNGNGIPDECEMDCNNNNVPDDCDVDPSDPDGNGLVSPDCNINGISDECEPDC
                     QPNGVADDCDIANGTSHDWNYDGIPDECQILGAAIYVDENALLSPHDGSDWDHAYLTL
                     QEGLADARANPEKVFIFVADGVYEPDDGTGQPGNRSLSFDLVNGFAMVGGFAGSGAAD
                     PFERQPQALEPLGHASVLSGDLLKNDQPGFVNNADNSYHVVTAGSTVTGSIILDGFFI
                     VGGNADGYEGGGGLSFRGGTSHEVSKSCRIVGHRRSDHPGRSLGSGDAQGSSAGARTD
                     HGGSQ"
BASE COUNT          918 a         1467 c         1395 g          995 t
ORIGIN      
        1 aacgacccgg ccgccccatg cgtgcgaacg ctccgccaag ccgtgatggt gaagtggttc
       61 tccggcgtct cacacgggtc cagcggatcg ggtgaaggtg cattatacgg cgtaggatca
      121 cacacgtcgc cgatgccatc ggcgtcgtcg tcggcctgat ctgaattctc ttggcagggg
      181 cagttgtcat cacaatccaa cgtgttacca ccggtgcagt tcggccgccc gaggacttgg
      241 ataccgtcgc cgtcgtagtc gaaattgttg acccacgggt cgggcggatt gtcacgggcg
      301 tcgagataac catggccgtc gcagtcgcag cagtcggaat ccgcgcgaat tcccggcccg
      361 ctggcgcact tctcgagcca atcgaggtcc ccaagtgtct ccgtcgggtt tccggggtct
      421 cgcatcttta cgtggccgtc gccgtccagg tcaaaggcgt cgcagtctcc gccagggaac
      481 agttgagcga tcgtgtacgt gttccccgtg taacaggctt gcatcagagc gaaatcgttc
      541 tggtcgacat cgccgtcgcg gtcgaagtcg ggaacggcca gatcgtaggg aacaatcacg
      601 ctgaagctgg gggagttcag gaccacgttc gccgcgctga ccggccaggc cgccggcggg
      661 ctcggcgggg gtgacaccac atccaggacg ttggccaagc cgttttcgat tcggaacacg
      721 tatgtccccg ccaccagggg gatggtgaat gagccctgtg cgatgatctg cgggccgctt
      781 tgccctattc caggatcaac aatgacgtct tcgccggtgc cggtgtctcc ggcttctccg
      841 agcgtgttct gagccccgcc gatttgacgg agattccttg cgaccggcgt gccgccgtaa
      901 cccgcgcccg gaacgtctgt ttcgggattg gagatcccgg ccgggcgaac aaagccgtcc
      961 attcccgttc cgacggttgt ggccggggac atgtccacaa gccggccgct tggcgggtcc
     1021 tggaccagat cgaccgaaat cgccgccaga cccgcgttgt cgccctggct caccgtcgca
     1081 ctgacggtcc agtagaccgt tccacccggc tcgacaactt gtcctgcatt cggagaggtg
     1141 agatccaacg tcacggttgc agcggtgctc tggccgccac caaacagccg ttgaaaatac
     1201 atgaaatcgt gcaagtcaac gtcgccatcc ggcggcgatg ccggaaaatc cgcgcaggtg
     1261 cagccgccgc caatcggatc ggtacgcggc ccggtcatgc acaccgagaa tccgtccagg
     1321 tcggagccat ccaggatgct gtcgcagttc aggtcaccca ccaccgggcc gcacgagccg
     1381 gcagtcacgg cgttcacggc ggtgagtgcg ttgaggtgct tgggttgatc ggggagttgg
     1441 caccagggat acgcccagtc ctgtcgtgtc gccgtgttgt agatgatggt tctggccaga
     1501 tccgcggtca gtgaaccgca gggaccgttc ttgtcgaaca tcaacgccgc cacacctgag
     1561 cagtgggcgg tagcctgcga cgtgccgtct ccggagaatc cccgacggtg atgctgaaac
     1621 ccggcgcggc caagaagtgc ccacagttgt ctgtggcatt ggaaaagcac accacccggt
     1681 agcgctctgg gttgaggtcg gcacaatctg ccggcggttc aaaatactgc ccggggtacc
     1741 agtcggcgtc gtaattcgcc gccaccggga ccgcacttga gaagcaggcc ggcatcgaaa
     1801 taccaccgca cacagcctcg ttgcccgtcg cagcgaaggt gatgatgccg ttggcaaaag
     1861 catcggatat cacctggttg taggctggcc accaagcgtc gcaagagcac tgataggccc
     1921 ccatacctcc gctgcggttg atcactcgga tacttgggtc tgcggtcaag gaacggttca
     1981 tggcatacag aatccccagc atgacatcag tcggattcgc gccgcccgaa ggcccgtgaa
     2041 cccggattga gagaatcttg gcgccggggg caacacccca tggatcggtg ataatcaacg
     2101 acatataggt tccatgatag aggctatata catcctcacc catgtcaggt tgtatgactc
     2161 caccctcgat gctaagacct cctgcggcga tgctcccaga aagccctgga tgcgtgtagt
     2221 cgattccggt atcgatcacg gcgacagtca ccccgtgccc gctcatgcct tgggcgtgga
     2281 catcgtccgc gttgataaat gatcgactct cgtgcatgcc aaacgccgca ctcgtcaaga
     2341 ccgatactgc cgccgttaca agaatacgac tctgcatgat gtctccctca ctttcaaatc
     2401 ggatcatggt tgtttacgcc atgctgcgct gggacgctcc aagcccatca ggccgattcg
     2461 gagtacgtcg gggtgtttcc acagcctttc cactccctgt ctgttgataa aacccgttat
     2521 tcccggcccg attctggcga ccaaccgaaa ctcgtccgcg ttcacaagag agagcacacg
     2581 atcctgaagc tcgttctctg cctgattacg aagctccaag cgtacgcggt tttctgaagg
     2641 taccttgctc cattctggaa ggaagtcggt tctcaagctg accataacct cgacatgatc
     2701 ggtcagggag aacgcgcgat acacgtcagg atccactttc ctctcttcca cgccaggctg
     2761 agtggaggcc gtatctgcag agtgtgcagg gggaaggtca tccttgtaga cggttcgcga
     2821 tttatcaggc agcggcttgt catccaggca gaccccggtt acgtcggggt tcttggccag
     2881 cttgtccagc ccctctttgg tgatgtagcc ggtcagaccc gcggatttct cgaacagaaa
     2941 cggagcgtgg aattccgagg ccttgaggct ggccagtacc cgtcgctggg catcacgttc
     3001 ctttaactgg acctgaacgt acaccatgcc cttgaagcgg aaccgccaca gggcgtcgaa
     3061 agcctccacg tccctaccct tcttcaggcg atccttgagc tcggcgacgg tcttgttcag
     3121 tcgatccagt ttggcgtaca cctctgggtc cacgacgccg gtccaagtcc gcggtgtgag
     3181 cccgagcttt tctctctcct cgcggggaat gatcgctcgg gttttctcgg ggtcattggg
     3241 atccgccgtg gtctgtacgc gccccggcac tggagccttg ggcatcgccg ctgccaagac
     3301 tgcggccagg atgatcactg cgccgatggc ccacaatcct gcaactcttt gaaacttcat
     3361 gacttgtacc tcctctgaat gagagaccgc cgccgccctc atagccatcg gcattgccgc
     3421 cgacgatgaa gaaaccgtct aaaatgatgc taccggttac ggtgcttccg gccgtgacca
     3481 cgtgatagct gttgtccgcg ttgttcacaa aacccggctg atcgttcttc agcagatccc
     3541 cgctcagaac gcttgcgtgg ccgaggggct caagggcttg cggctggcgt tcgaacgggt
     3601 ctgcggctcc ggagccggcg aaaccgccga ccatggcgaa gccgttgacc aggtcgaacg
     3661 acagcgatcg atttccgggt tgtcccgtgc cgtcatcggg ctcgtagaca ccgtcggcca
     3721 cgaagatgaa gaccttctcg gggttggcac gagcgtcggc caagccctcc tggagcgtga
     3781 ggtaagcgtg gtcccagtct gaaccgtcat gcggagagag caaggcattc tcatcaacat
     3841 agatggccgc accgaggatc tggcactcat ccggaatacc gtcgtagttc cagtcgtggc
     3901 tcgttccgtt ggcgatgtcg caatcgtcgg ccacgccgtt cggctggcag tcaggctcgc
     3961 attcatcgga tattccgttg atgttgcagt cggggctcac cagcccgttg ccgtccggat
     4021 cggaaggatc gacgtcgcag tcgtccggca cgttgttgtt attgcagtcc atctcgcatt
     4081 catcaggtat gccattcccg ttgcagtcct cgctggtgct gttggcaatg tcgcattcgt
     4141 ccggcacgcc gttgccgttg cagtcgccgg acgtacctgc cgacaagaag cactggaccc
     4201 cgccgccgaa ctggtcggga agggacgcga tttcaaagag gatgccatct gtagacaccc
     4261 atacttctgc aataccatcg cccgcataat aaccgtcagg caacccaaga ccgacgaaaa
     4321 gacgaccttg gtgagcgacc agagccgaga cgccctcatt gttggtggta ctgtctggat
     4381 gggcaaacac cagtgaccat tcgtgcccgt caaacgacca tagtctcgct ccatttcgat
     4441 caacgccccc tcctgcgtag agctttgcct tgggtattgc ggtgcctcca aacgtctcaa
     4501 gtgcccacac gttcacaccc ctgttgttgt tgttctgtgc atagttgacc gtgaagtcta
     4561 cacgtggcag gctcgatcgc agttggtcac acaggaagtc gggttcggtg cgatagacag
     4621 gacctccgtt gccggcccac gcacctgcat acaggtgccc cgtatcgtaa tctgagtcgt
     4681 attctgcgaa atcccatatg caactccccg actgtgtgtc gatgtcggcg ccggtctgaa
     4741 catgctgtaa atccgccggt ttgatgatgc ggtga
//
LOCUS       DAOTPW010000338         4766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_14718_length_4766_cov_5.490554, whole genome shotgun sequence.
ACCESSION   DAOTPW010000338 DAOTPW010000000
VERSION     DAOTPW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14718_length_4766_cov_5.490554"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="P5316_20360"
     CDS             complement(<1..1071)
                     /locus_tag="P5316_20360"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR87369.1"
                     /translation="MRWRNGGIGMHWAEAYPRMDVDWAQVELVLRRKVPDQVPVVELF
                     TDHDFIQAVLGYHWTAVAANRDYAEWQRYWLWRIEYQKLAGPAYINAGMDGLVYPVRK
                     VACATNTAELALGDRAWVNETEGVITSREDFEAYPWPTSPFNRRQTDFIAENAPPGMG
                     IIMTSSGILEWAMWLMSFETFSMALHDQPDLARDVVNRVGERFLDFYRQAAQHEAVRA
                     VWMGDDLGFKTATMISPQHLREYIFPWHRRIAEAAHAAGKPYLLHACGNIDAVMEDLI
                     EYVGIDARHSFEDVIEPVTSVKRRYGHRIAIIGGVDVDFISRRSEQEVRQYVRRILEE
                     CTPGGEYAVGTGNSVANYIPIRN"
     gene            1518..2624
                     /locus_tag="P5316_20365"
     CDS             1518..2624
                     /locus_tag="P5316_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87370.1"
                     /translation="MNDFRDLGQGSKMRAASPILVVAEPDKSSGDSWRRFLAVLCFME
                     FYTGLVRSGTILWESWSDDFSPLSANPMFWANAILLFRAFCLYICGRALWFGSKHAGR
                     WVFIALVLTLVTLIWVAWSDYRAFCEIRGSSLPIWPARPWANLSYILRNAVQMVPLLT
                     CFVISLLFRRSATGKGEPHTARWVVLASAWCFAVAVEELLVGNAGAFLAFCTQSIHLE
                     TGALYVYAAVVAGTGVLLILKAVPARTAALILAAIGVVYAAEESFVLPWLVNIAIHAL
                     YLSVPGYPGGLSYRWVHTRQEFLGVFVDSVRLVGPWLLIALYARFVPMRTPPDDGSPY
                     PRRYCRKCLYNLHGLESDRCPECGTELGTGSPTA"
     gene            2931..>4766
                     /locus_tag="P5316_20370"
     CDS             2931..>4766
                     /locus_tag="P5316_20370"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRR87371.1"
                     /translation="MRTHPFAPLGFSGVIVAAVLLAVVPAVAAQDGSDHWCELHGEGK
                     DGLCGRAMLAEAYFEQLGRLPEAAGDATEGVDDTDITHCLLDIEVNPVTEVVSGSNTL
                     SITSRIDGLSAITLDLRDNMTVSNVTIGGASMGFTRPGHQVTITLDRSYNTGESFQVK
                     IDYSGTPQNLNWGSFDFGTHAGTAVVASLSEPWYAHTWWPCKEDIHDKFTMDMWVTVP
                     NWMIVASNGALQGTDTLTGSRKRFRWRESYPISVYLVSVAMTNYTKWTEWYNHAGGSM
                     PVEFYIYPEELASVQPLLVDVVTMIETLSAPATFGEYPFINEKYGIAQFEWCCGMEHQ
                     TITSQGSFPERRTVHELAHSWWGNDLTCKTWHDIWLNEGFARYAEALWYERRPGGSYA
                     AYLQHLQEYRPTSNGQYGTVYRYDISTPDLIFSSTYSYNKGAWVLHMLRHVLGDATFF
                     NVLASYRQMYTGKAADTDEFAAVVAAVSGRPIDWFFDQWVYDDGAPYYRFGWETEQIG
                     QQHWARVCVEQYQTAYPNFRMPIDITVTSSGTPVTHVVEQQADTQWYLLPADGPVTNV
                     QFDKDTWILRGAGTAVSYIEGPPKLLATSPAPGTTFDPAPEITAIE"
BASE COUNT          860 a         1518 c         1448 g          940 t
ORIGIN      
        1 gttgcggatc gggatgtagt tggccacgct gttgccggtg ccgacggcgt attcgccacc
       61 gggtgtgcat tcctccagga tccgccgcac gtactgccgg acctcctgct cgctgcggcg
      121 gctgatgaaa tcgacgtcca cgccgccgat gatcgcgatg cggtggccgt atcgccgctt
      181 gaccgaggtc accggttcga tgacgtcctc aaagctgtgg cgggcatcga tgcccacgta
      241 ctcgatcagg tcttccatca ccgcgtcgat gttcccgcag gcgtggagca ggtacggttt
      301 gccggccgcg tgggccgcct cggcaatccg ccggtgccac gggaagatgt attcgcgaag
      361 gtgctggggc gagatcatcg tggccgtctt gaaacccaga tcatcgccca tccataccgc
      421 ccgcacggcc tcatgttgtg ccgcctgccg gtagaaatcg agaaaccgct cgcccacgcg
      481 attgacgaca tcgcgggcca ggtccggctg atcgtgcaaa gccatggaga acgtctcgaa
      541 gctcatcagc cacatcgccc actcaagaat gcccgacgat gtcatgatga tgcccatgcc
      601 cggcggggcg ttctcggcga tgaaatcagt ctgccgccgg ttgaacggcg acgtcggcca
      661 ggggtatgcc tcgaaatcct cgcggctggt gatgacaccc tcggtctcgt tgacccaggc
      721 gcgatcgccc agtgccagtt cggcggtgtt ggtcgcgcac gccaccttcc gcacggggta
      781 gaccagcccg tccatgcccg cgttgatata cgccggcccg gccagtttct ggtactcgat
      841 ccgccagagc cagtaccgct gccactccgc gtaatccctg ttcgcggcca cggccgtcca
      901 gtgatagccc agcaccgcct gaatgaagtc gtgatcggtg aacaactcga ccaccggcac
      961 ctgatcgggc actttgcggc gcagcacaag ctccacctgc gcccagtcca cgtccattcg
     1021 gggatacgcc tcagcccagt gcattccgat gcctccgttg cgccatctca tggtaatcca
     1081 tcggccgggc cgggcaatcg ggtccgtgat gcattcgggg tctctttcag aaccccgagc
     1141 gtacctgtcc gccgtagcct tggcgaaggc ggaagcgagg ggcccttgac cgattcccac
     1201 aagcgggtca agggcaggcc tgacaacaac gtggccgcac cacaaaaagg ccgctccctt
     1261 acggtcgcgg ttctgacaga accgcgacca gtgccacgga cccggggcaa tcttgctctg
     1321 cctcgcctcc tttccccgat gacggtcagg cattatggta aacgccgctc ccagaaacgg
     1381 gccgctggtc ccagattccc atctgggacc tctcgggtga cgattcccat cgtcattggc
     1441 gccgaatgcg gaaaggattc cgcgaacgag tgcggcccag aagcgttctg ggccaagcag
     1501 aaacggacag gttgggcatg aacgattttc gcgatctcgg gcaggggagc aaaatgcgtg
     1561 cagcttcacc gatcctcgtc gtcgccgaac ccgacaagtc aagtggagac tcctggcgcc
     1621 ggttcctcgc cgtcctgtgc ttcatggagt tttacacagg acttgtccgc agcgggacaa
     1681 tactgtggga atcttggagc gatgacttct cgccgttgtc cgccaacccg atgttctggg
     1741 cgaacgcgat cttgttgttt cgtgctttct gtctgtatat ctgcggaagg gccttgtggt
     1801 tcggatcgaa gcacgccggc cgttgggtgt tcatagctct cgtcctcaca ctggtcacct
     1861 tgatctgggt tgcctggagc gactacagag ccttttgtga gattcgcgga tcatcgcttc
     1921 cgatctggcc ggcgcggccc tgggccaact tgagttacat cctgagaaac gcagtccaga
     1981 tggttccatt gctgacctgc ttcgtcattt cgcttctctt ccggcggtcc gccactggaa
     2041 agggtgagcc tcacacagcc cgctgggtcg ttctggcgag cgcatggtgt ttcgccgttg
     2101 cagtggaaga actgctcgtc ggcaacgcag gcgcgttttt ggccttctgc actcagagca
     2161 ttcacctgga gaccggtgcc ctgtatgtct atgcggccgt cgtggccggt acgggtgtgc
     2221 tgctaatctt gaaagctgtg cccgcacgca ccgcagccct gatactggct gccatcgggg
     2281 tagtatacgc cgcagaagag agcttcgtcc tgccgtggct cgtcaatatt gcgatccatg
     2341 ccttgtacct gagcgttccc ggttatccgg gcggtctttc atatcgctgg gtccacaccc
     2401 gccaggaatt cctcggtgtg ttcgtcgact ctgtcaggct ggtcggccct tggttgctga
     2461 tcgccttgta cgcccgtttc gtgccgatgc ggacgccgcc ggatgacgga tcgccctatc
     2521 cgcggcgcta ctgccgcaag tgcctgtaca acctgcacgg cctcgagtcc gatcgctgcc
     2581 ctgagtgtgg aaccgaactc gggactgggt cgccgacggc gtaggccgga ccggatggtt
     2641 tttgccctcc cttgtgccca gcggtagggt ccgccctggc ggaccgcgcg gctctctcca
     2701 gagacgctgt tagcatggga aagcccggat ccaaagaaga aaccgctcgc tcagggagaa
     2761 tactgctatc ggtccggggc ctccatgagg tagaatggca gttcggcggt cggccccgga
     2821 tgcggccggc cggacagtgt cggaaatgcc aggtcgcacg gtttgtcgat cgcgttcgac
     2881 tggatgaatc cggtccgcat gtcggtattc agatcgcgga ggacggaagc ttgcgaacac
     2941 atccgtttgc cccattgggt tttagcggcg tcatcgtggc ggcggtattg ctggcggtcg
     3001 tgcctgcggt cgccgcacag gacggctcgg atcattggtg cgagcttcac ggcgagggga
     3061 aggacggtct gtgcggccgt gcgatgcttg ccgaagcgta tttcgagcaa ctgggcagac
     3121 tgcccgaggc ggccggcgac gccaccgagg gcgttgacga caccgatatc acgcattgcc
     3181 tcctggacat cgaggtcaac ccggtcaccg aggttgtttc cggatccaac acgttgagca
     3241 tcaccagccg aatcgatgga ttgagcgcga tcacactgga tctgcgcgac aacatgaccg
     3301 tcagcaacgt gacgatcggt ggggcttcga tgggcttcac gcgcccgggc catcaggtga
     3361 ccattactct cgatcggtcc tacaacaccg gcgagagttt ccaggtgaag atcgactact
     3421 ccggcacgcc tcagaacctg aactggggct cgttcgattt cggcacacac gccggtacgg
     3481 cggtcgtcgc ctcactgagc gagccgtggt acgcccacac ctggtggccc tgcaaggagg
     3541 acatccacga caagttcacg atggatatgt gggttaccgt gccgaactgg atgatcgttg
     3601 cgtcgaacgg cgccctccag ggcaccgaca cgctcaccgg cagccgcaag cgattccgat
     3661 ggcgcgagag ctatcccatc tccgtctatc tcgtttcggt ggcgatgacc aactacacca
     3721 agtggactga atggtacaac cacgcgggcg ggtcgatgcc ggtcgagttc tacatctatc
     3781 cggaggagct cgcctcggtc cagccgctgc tggtcgacgt cgtcaccatg atcgagacgc
     3841 tcagcgcacc ggccacgttc ggcgagtacc cgttcatcaa cgagaaatac ggcatcgccc
     3901 agttcgagtg gtgctgcggc atggagcacc agacgatcac cagccagggc tcctttccgg
     3961 aacgccgcac ggtacacgaa ttggcccatt cctggtgggg caacgacttg acctgcaaga
     4021 cctggcatga catctggctt aacgagggtt tcgcccgcta cgccgaggcg ctgtggtacg
     4081 agcgcaggcc cggaggcagc tacgcggcgt atcttcagca cttgcaggaa taccgcccga
     4141 cttccaacgg ccagtacggg accgtctacc ggtacgacat cagcacaccc gacctcatct
     4201 tcagctcgac ctactcctac aacaaggggg cgtgggtgct gcacatgctg cgtcatgttc
     4261 tcggtgacgc aacgttcttc aacgtcctgg caagctatcg ccagatgtat accggcaagg
     4321 cggccgacac cgatgagttt gcggccgtgg tcgccgccgt cagcggccgg cccatcgact
     4381 ggttcttcga ccagtgggtc tacgacgacg gcgctcccta ctaccgtttc ggctgggaga
     4441 ccgagcagat cggccagcag cactgggccc gcgtatgcgt cgagcagtac cagacggcct
     4501 atccaaactt cagaatgccg attgacatca cggtgaccag cagcggcact cccgttaccc
     4561 acgtcgttga gcagcaggcc gatacccagt ggtatctgct gccggccgac ggcccggtca
     4621 ccaatgtcca gttcgacaag gacacctgga ttctgagggg cgcgggaacc gcagtgagct
     4681 acatcgaagg gcctcccaag ctgctcgcga catcgcccgc cccgggcacg accttcgacc
     4741 ccgcccccga gatcaccgcc attgaa
//
LOCUS       DAOTPW010000339         4678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15070_length_4678_cov_5.577331, whole genome shotgun sequence.
ACCESSION   DAOTPW010000339 DAOTPW010000000
VERSION     DAOTPW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15070_length_4678_cov_5.577331"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="P5316_20375"
     CDS             <1..237
                     /locus_tag="P5316_20375"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HRR87372.1"
                     /translation="TARKQPCEPIAVVGRVSMDQINLDLTDAGPVAVGDRVVIIDDDP
                     SSFNSVESLATMTESIPYEITTLIGRRVRRVSVP"
     gene            complement(351..1370)
                     /locus_tag="P5316_20380"
     CDS             complement(351..1370)
                     /locus_tag="P5316_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87373.1"
                     /translation="MPETPIRNRSISLTSFLTALGAVIAGCILIIAGLVPSDLTGRAV
                     LIVGGMLLIAVSLLLHSLVKLLVLTEANINRVHHNTLDLHDTARRLEPMIKQIADNSQ
                     LSDAARSITHRDKELEALRHALREEMFQGNWDAAMYLIEQMQTRFGYAREAEALRKEL
                     AQIREMTIEEKIGDAVSHITKLMDEHHWERARQESERLIKLFPRHERVLGLPAELNRR
                     REARKQELLQQWNQAVAREEVDRGIQILTELDQYLTKEEAQSLQDSARHVFKARLVAL
                     GVSFGLAVSENRWRDALEVGLCIRQEFPNSRMAQEVGEKLDTLRIRAGFAAGAEVIQR
                     SVTQE"
     gene            complement(1688..1921)
                     /locus_tag="P5316_20385"
     CDS             complement(1688..1921)
                     /locus_tag="P5316_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87374.1"
                     /translation="MDAQSLLTVLCILLGTGLFVRMVAKEKHRRDKHLELRRYEKELA
                     EAEQQKRMARQAASEGAPVVHVAELRPQNSDKP"
     gene            2216..2443
                     /gene="infA"
                     /locus_tag="P5316_20390"
     CDS             2216..2443
                     /gene="infA"
                     /locus_tag="P5316_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRR87375.1"
                     /translation="MAQSEKIQVEAKVIKALPNAQFLVEAETGNAKHEVLAHIAGKMR
                     KHFIRIVPGDIVTVELSPYDLTRGRIIYRQR"
     gene            2609..4435
                     /locus_tag="P5316_20395"
     CDS             2609..4435
                     /locus_tag="P5316_20395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HRR87376.1"
                     /translation="MALGERISNELLPQVRQPGQYIGGEWNQLVRPGDWERAELRVAL
                     AFPDTYAVGMSHLGCQILYWLCNHTPGVCAERVYCPWTDAERVMRGRGIPLFTWDTRQ
                     PVATADILAVSMQYEMSSSNLLLLLDLAGIPLGSADRDEGHPLVIVGGPQMDNPEPIA
                     DFVDAVVIGDGEASTAAILDACRELKRCGASRSERLIELARRFEWLYVPSLYEIKYRL
                     DGTIAGMVAASGCPAGFAPRQTIVRCFTKDFDNAPVPIRPIVPFTEVVHDRISIEIMR
                     GCPKRCRFCHAGYTKRPMRIRSVDRIMEIAESAWRATGHDEIGLLSLSSADYPRLREL
                     AERMNERFGPRRVNLSLPSLRVDKMLSDIPWLVSSVRKGGLTIAVEAATQEMREAIRK
                     EVLEGDLIEGIRQAYKAGWNSVKLYFMCGFPGETESDIRGIFDLALQVSHAKRAVRGG
                     PASVNAGVSWLVPKPHTPLQWAPMQTAEYFHEARRILLDAARQRRSAVHIKTHRVDRS
                     ILEGVFARGDRRLGRVIETAYRLGARMDGWDEVFDNSLWLRAFEMTGIDPAFYAHRRR
                     DAAEIFPWDHIESGPPRGRLLAQYEDMLARCAARIDRSLETG"
BASE COUNT          864 a         1415 c         1469 g          930 t
ORIGIN      
        1 actgcccgca agcagccctg cgagccgatc gcagtggtcg ggcgggtcag catggaccag
       61 atcaacctgg atctgactga cgcaggcccc gtagcggtgg gggatcgagt ggtcatcatc
      121 gatgacgacc cctcttcatt caacagcgtc gagtcgctgg caaccatgac cgaatctatt
      181 ccctacgaga tcaccacgct gatcgggcgt cgtgttcgcc gtgtcagtgt cccatgagca
      241 gcatgtctct cgataaccaa ggccaacccg gaacatcgac gagaaggcac cggacgcaac
      301 tcgtcggcag gcgcatgaca gagcgcggtt caaacacata gctgagaacg ctattcctgg
      361 gtaaccgatc gctggatgac ctcggccccg gcggcaaaac ccgcgcggat acgaagtgta
      421 tcgagcttct ctccgacctc ctgggccatc cggctgttcg gaaactcctg acgaatgcac
      481 agaccgactt cgagcgcatc gcgccagcga ttttccgaca cggcgagtcc gaagctgacg
      541 ccgagcgcga ccagacgagc cttgaatacg tgacgggcgg agtcctgcag actctgcgcc
      601 tcttctttcg taaggtactg gtccagctcg gtcaggatct gaatgccacg gtcgacctct
      661 tcccgggcaa cagcctggtt ccactgctga agcagttcct gttttcgggc ttcccgtcgc
      721 cgattcagtt ccgcgggaag ccccaggacc cgctcgtgac gggggaacag cttgatcaac
      781 cgctcggact cttgacgggc ccgctcccaa tggtgctcat ccatcagctt ggtgatgtgc
      841 gagacggcat ccccgatctt ctcctcgatg gtcatctcgc gaatctgggc aagctctttg
      901 cgaagggctt cagcctcacg agcgtacccg aaccgcgtct gcatctgctc aatgaggtac
      961 atcgcggcgt cccagttgcc ctgaaacatc tcttcacgca gggcatgacg gagggcctcg
     1021 agttccttgt cccgatgcgt gatcgaccgg gccgcgtcgg atagctggct attgtcggcg
     1081 atctgtttga tcatcggctc caatcttctg gccgtgtcgt gaagatccag ggtgttatgg
     1141 tgaacgcggt tgatgttcgc ctcggtcagg accaacaact tgaccagcga gtgcaacagc
     1201 agcgaaacgg cgatcaacaa catgccgccg acgatcagga cggcccgccc ggtcagatcg
     1261 gacggcacca ggccggcgat tatcaggatg cagccggcga tcacggcacc aagggcagtc
     1321 agaaacgacg tcagactgat tgaacgattc cggattgggg tctcaggcac gatccttcgc
     1381 tcctgacgtt cgccgacttg acggcgccgc cacgtgacat acgcggcgtt ttcactctaa
     1441 ggcttcaagc ggcaccagtc aacctgccgc gtcccggtgc cctcaggctg cgcaacagat
     1501 cgggcgacgg cgcacaacag gccgttcagg gggggcgttc agaacagcga ccccggtttg
     1561 ccaaggcgac cgtgcctgtc cggcttggcg cgccgaccct gcccaaaggc gagagtctgt
     1621 cacgtgggat cgttttcccg gaaggaggtc gtcggagtgc ttggggcaag gagcgatgca
     1681 aagggcttca gggtttatcg gagttttggg gccgcagttc ggcgacatgg acgacggggg
     1741 cgccttccga ggccgcctgc cgggccatgc gtttctgctg ctcggcctca gccagttctt
     1801 tctcgtatcg tcgcaattcg aggtgcttgt cgcggcggtg tttctccttg gccaccatcc
     1861 gcacgaaaag gccggttccc agcaggatac acagaacggt taacagagat tgcgcgtcca
     1921 taaacggcat tgaagatgac cctgcagcgg ttggccctca gcgggcgggg tgcccgtcca
     1981 ggggatctat cggaaagagc aggtcggggc ttcatttgga ggggccggca gggcaccttt
     2041 ttccggaccg gacgcggctc ggcggcgaga acgcggtcct gtgatgcccc tgaggcgtcg
     2101 acggtccggg ccgtcgtgga aagcggttgg gttattgatt tgcttattgc gcggcgttgc
     2161 cgataatata cccccacaca ggtttgtggt ttcgctgaca acgacaaggg agtttatggc
     2221 gcagtctgag aagatccagg tcgaagccaa agtcatcaag gcgctgccca acgcccagtt
     2281 tctggtcgag gcggaaacgg gcaatgccaa acacgaggtc ctggcccaca ttgccgggaa
     2341 gatgcggaaa cacttcatca ggatcgtacc gggcgacatc gtgacggtcg agttgtcccc
     2401 ctatgacctg actcgcgggc gcatcatcta tcgccagcgt tagtttcctt cagcctcgcg
     2461 atcccacctc gatccacacg tatctccggt aatccgatcc gtcagggaac gtgctgtgcc
     2521 gcgggctcgg ctgtccggca aacccatcga acggtagaat aagcggcaca gccgcgcggt
     2581 ggtcggcgcg gatgcgtgga acgttgccat ggcgcttggt gagaggatca gcaatgagct
     2641 cctgccgcag gtacggcaac cggggcagta catcggcggc gagtggaacc agcttgtccg
     2701 gcccggcgac tgggagcgag cggagctgcg cgtcgccctg gcttttcccg acacctatgc
     2761 agtcgggatg agccacctcg gatgtcagat tctctattgg ctgtgcaatc acacgcccgg
     2821 tgtctgtgcc gaacgcgtct actgtccctg gacggacgcg gagcgggtga tgcgcgggcg
     2881 gggcattccg ctgttcacct gggatacccg acagcccgtc gcgacggcgg atatcctggc
     2941 cgtgtccatg cagtacgaga tgtcctctag taaccttctt ttacttctcg acttagcggg
     3001 cataccgctc gggtcagccg atcgtgacga gggacatcct ctggtcatcg tgggcgggcc
     3061 tcagatggac aatcctgaac ccatcgccga ttttgttgat gccgtcgtga tcggggacgg
     3121 cgaggcaagc acggccgcga ttctcgacgc gtgccgtgag ctcaagcgtt gcggcgcgtc
     3181 acgttctgag agactcatcg aacttgcccg ccggtttgag tggctctacg tcccgtcgct
     3241 gtatgagatc aaataccgac tcgacggcac gattgccggt atggtcgccg caagcgggtg
     3301 tcctgccggc tttgcaccgc ggcagaccat cgtccgctgc ttcacaaagg atttcgacaa
     3361 cgcccccgta ccgattcgtc cgattgtgcc gttcacggag gttgttcacg atcggatcag
     3421 catcgagatc atgcgtggtt gtcccaagag atgccgattc tgccacgccg gctacaccaa
     3481 gcggccgatg cgcatccggt cagtcgatcg tattatggag attgccgagt ctgcctggcg
     3541 ggccaccggt catgacgaga tcggcctgct gagcctgtcc agtgcggatt atccgaggct
     3601 tcgcgagctg gcggagcgga tgaacgagcg gtttgggccc cgccgagtga acctctctct
     3661 tccgtcgctt cgggtggaca agatgctttc ggacattccc tggctggtga gcagcgtccg
     3721 caagggcggg ttgacgatcg ccgtcgaggc ggccacacaa gagatgcgtg aggcgatccg
     3781 caaagaggtg cttgaagggg acctcatcga aggaatccgg caggcctaca aggccggctg
     3841 gaactcggtg aagctttatt tcatgtgcgg cttcccgggc gagaccgagt ccgacatacg
     3901 cggcatcttc gatctagctc ttcaggtgag tcacgccaag cgggccgtcc gtgggggccc
     3961 ggcctcggtc aacgccggcg tgagttggct ggtacccaaa cctcacacgc cgctgcagtg
     4021 ggcccccatg caaacggccg agtatttcca cgaggcccgc cgtatcctgt tggacgccgc
     4081 acggcagcgc cgctcagcgg ttcacatcaa aacccatcgc gtcgaccgct ccattctcga
     4141 aggcgtcttc gcccgcggcg accggcgtct gggtcgcgtc atcgaaaccg cgtatcgcct
     4201 cggtgcccgc atggacggct gggacgaggt tttcgacaac agcctctggc tcagggcgtt
     4261 cgagatgacc ggcatcgatc cggccttcta tgctcaccgc cggcgtgacg ccgctgagat
     4321 ctttccctgg gaccacattg aaagcggccc tccacgaggc aggctcctgg cccagtacga
     4381 ggacatgctg gccagatgtg ccgctcgcat cgaccgctcc cttgagactg gctgatcggg
     4441 catgccgacc tcgatgatag agtctttagc ctggatactc ttctagggaa gccctcttgg
     4501 ccaagaagcc ggattgggtt tctttcaccg gggcggatca gggcgcgtac gaaccttgct
     4561 cgcagacgtc gggttcttca ttaagtggtt gcaaaggaaa gacttaagga catgaatggg
     4621 tttgtttggc gcggggggtg gtcggcggtc ggtgtccggc ttttggcgcg cgggggtc
//
LOCUS       DAOTPW010000340         4678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15071_length_4678_cov_5.566948, whole genome shotgun sequence.
ACCESSION   DAOTPW010000340 DAOTPW010000000
VERSION     DAOTPW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15071_length_4678_cov_5.566948"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..2128
                     /locus_tag="P5316_20400"
     CDS             296..2128
                     /locus_tag="P5316_20400"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRR87377.1"
                     /translation="MKREPEASSRRQGGWNTMVFWATAAFGATFAVSVMAVDYPIEIH
                     RGNGLANVFAKLNSGHSVKIAYIGGSVTEMTGYRNNVTSWFNSKYPGRITEINAGWSG
                     TGSLIGAMRYARDVLAQNPDLVFIEFAVNDLPEDPVSFIERNSEGMVRQSWTQNSMAD
                     VCFIETIAYYNEPAYLAGYYPNSVQAHYNVCDHYGVSSVNVGWALYEHVLAGTPWESL
                     TIDGDRVHPNASGSQIYSNAIVSYLESERVRGAAAAAHNLPSPRTDYPVTSSTITDWV
                     TVSPLPSGWTAHYNEFGVPSFIQSSTAGSQVSIGFTGPSAAVKIVVSGDSGPRLYYSV
                     DGGAFATAYLAVNGYTYLWALPVAKNLSTGSHTLTIRVDTGVVRLINVESAQTGGGGP
                     LPGDVNLALTAVADATDSVYGPDWTGAKARDGLLNTKWTSTGTTPNHWLALDLGAIAD
                     VSCVVVKHAGAGGEPSYYNTQVFTIESGSSMAGPWTTQFTGSNPYQLHTTLFTFAEPQ
                     QIRYVRLNISDAGVDNYARIPEFEVWGTIPPAKGDMDLDNDVDQHDFGLFQACYTGAG
                     NPQNLSQCAPARLDDDNDVDSEDFAIFRACLTGPGVPADRDCGR"
     gene            complement(2366..4456)
                     /locus_tag="P5316_20405"
     CDS             complement(2366..4456)
                     /locus_tag="P5316_20405"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRR87378.1"
                     /translation="MTRRYVWMWLFISAALVSAAQAESITTGSLLREMIDLHKLADFP
                     SPAYKTVQFSSYDHRSKVPGGPEWFANSDGFGREPVPNFEAVIREPADGKPGEYLICD
                     VKGPGAIVRTWTAAIRGNIRLFLDDMDKPLFDGPAEEFLYRPYEPFSKEAGIDPDFYR
                     NSFQQNRAGYAPMPFAKQCRIIWVGNVNEIHFYQIQIRLYEPTANVVTFGPQDVKKYA
                     NEIREIGKVLAAPAANWKYASTAAPVEIKADLAPGEVREVLKLKGPQAVERLTLKLSA
                     KDIDRALRQTVMHVICDDYPWGQVQSPVGDFFGAGPGINPYDSVPFTVEADGTMTSRF
                     MMPFAKSMRIVLQNLGQQAASISGSVLPMDYEWNDKTSMHFRARWRVNHDLVGAGDAV
                     QDLPFLIARGGGVYVGTASLLLNPTEIPTPGGGWWGEGDEKIFVDDDVRPSTFGTGSE
                     DYYNYAWSSPDIFFHAYCGQPRNDGPGTRGFVTNNRWHVIDALPFKNSIALYMELFHH
                     ERTPGISYARVGYHYARPGLIDDHVPITGEDVRSLDLPPNWQPASRGGANNSVYHQAE
                     SLVHSPRAKLADGNLYAGGKVLQWEPASKGDELVLTFPIAEAGRYALHLAAALNGESG
                     RISVHMGDKSIMGGEKGVIDLCAPGRQMLREFSTGPMDLPKGDLKLTVRYEGPSEKCG
                     GSTVGLDYLRVQRR"
BASE COUNT          897 a         1484 c         1405 g          892 t
ORIGIN      
        1 caaatctggc tggtcagcca ctatctcttc agaaaccgac cgcccggcct cttttaccac
       61 gcctcggagt tgagtaaact gcaatcggta tgcgagggca tcagggagcg attccaaaag
      121 ccctcgatca atgaatagag cctcagggct ggaaagcgat gcggcttgcg cgcttgtttc
      181 cggactcaaa tgcgcttcgg ttgcggcccg caaaaaggcg gtccgcctta ggcggaagtt
      241 ccggattcgc gatcgtcacg caacagtccg catcacgatg aactggagtg gagcggtgaa
      301 gcgagaacct gaagcctcct cacgtcggca aggcggctgg aacacgatgg tcttttgggc
      361 gaccgcagct ttcggcgcga cgtttgcggt atcagtgatg gccgtcgact atcctatcga
      421 aatccatcgc ggtaatggtc tggccaacgt ctttgccaag ctcaacagcg gccactcggt
      481 caagatcgcc tacatcggcg ggagcgtcac ggagatgacc ggctaccgaa acaacgtgac
      541 ctcgtggttc aactcgaagt accccggccg tatcaccgag atcaacgccg gctggagcgg
      601 gacaggcagc ttgatcggag ccatgcgcta tgcccgcgac gtgctggccc agaatcccga
      661 cctggtcttc atcgagtttg cggtcaatga cctgcccgag gaccccgtat cattcattga
      721 acgcaacagc gaagggatgg tccgccaaag ctggacgcag aactcgatgg cagacgtgtg
      781 cttcatcgag accatcgcct actacaatga gccggcctac ctcgccggat actatcccaa
      841 cagcgtgcag gcccactaca acgtctgcga ccattatggc gtgtccagcg tcaacgtcgg
      901 ctgggcgttg tacgagcacg tgctggccgg gacaccttgg gagagcctaa cgatcgacgg
      961 cgaccgcgta cacccgaacg cctccggctc gcagatctat tccaacgcga tcgtctctta
     1021 cctggaaagc gaacgcgttc gcggggctgc tgctgctgcc cacaacctgc cctcccctcg
     1081 aaccgactac ccggtcacga gtagcaccat caccgactgg gtcactgtca gcccgctgcc
     1141 gtccggctgg acggctcact acaacgagtt cggcgtaccc agcttcatcc agtcatccac
     1201 ggccggatca caggtcagca tcggcttcac cggtccgtcg gcggcggtga agatcgtcgt
     1261 gtcgggcgac tcggggccca gactgtacta ctcggtcgac ggtggtgcgt ttgccacggc
     1321 ttacctcgcc gtgaacggct atacctactt gtgggccttg ccggttgcca agaacctctc
     1381 gaccggttcg cacacgctga ccattcgggt ggacacgggt gtggtccgcc tgatcaacgt
     1441 cgagagcgcc cagaccggcg gcggcggacc gcttcccggt gacgtcaatc tcgctctgac
     1501 ggccgtggcc gacgcgaccg acagcgtcta cggtcccgac tggaccggcg ccaaggcccg
     1561 tgacggactg ctcaatacca agtggacttc gacgggcacg acgcccaacc actggctcgc
     1621 gctggacctc ggggcgattg ccgatgtgag ttgtgtcgtg gtcaagcacg caggcgccgg
     1681 tggcgagccg agctactaca acacgcaggt cttcacgatc gagtcgggga gcagcatggc
     1741 cggcccgtgg acgacgcagt tcaccggctc gaatccttat cagctccaca ccaccctgtt
     1801 cacgttcgcc gagccgcagc agattcgata cgtccgcctt aatatctccg acgccggcgt
     1861 ggacaactac gcccgtatcc ccgagttcga ggtctggggc acgatcccac cggccaaggg
     1921 cgacatggac ttggacaatg acgtcgacca gcacgacttc ggcctcttcc aggcgtgtta
     1981 caccggcgcg ggcaaccctc agaacctctc tcagtgcgcg ccggcccgtc tggatgacga
     2041 taacgatgtg gattccgaag acttcgctat cttccgggcc tgcctgacag gccccggcgt
     2101 cccggccgac cgcgattgtg gcagatgaga atgtgcgaaa cgatgtgccg cctgtgcgca
     2161 gatcagagcg ccgtcttcag aaccgcgatc ctggttcgcc gagacgaccg cacccgtgct
     2221 gcgtagcctt ggcgaagcag aaaggcagcg gcagcaaccc agccgaccgc tgaacgctga
     2281 ccgctctttc agcaccacag atttcgcagt ggcgggcgtc tccccgtcca tgcgtttctg
     2341 cccgactgcg atcgtgcgca caccctcacc ggcgctgcac gcgcaggtag tcgaggccga
     2401 cggtgcttcc gccacatttc tctgacggcc cctcgtaacg gacggtcagc ttaagatccc
     2461 ccttgggcag gtccatcggc cccgttgaga actcccgcag catctgccgg cccggggcgc
     2521 acagatcgat cacgcctttc tcgccgccca taattgattt gtcgcccatg tggaccgaga
     2581 ttctccccga ctcgccgttc aaagccgcgg ccaggtgcaa ggcatagcga cccgcttcgg
     2641 cgatgggaaa ggtcaacacg agttcatcac ccttgctcgc cggctcccac tgtaagacct
     2701 tcccaccggc atacagattg ccatcggcaa gcttggctcg tggtgagtgt accagcgact
     2761 cagcctgatg gtacaccgaa ttgttcgcgc cgccgcgtga ggcgggctgc caattgggcg
     2821 gcaggtcgag cgaccggacg tcttcgccgg tgatgggtac atgatcgtcg atcaagcctg
     2881 gccgggcgta gtgatagccg actcgggcgt acgatattcc cggcgtccgt tcgtggtgga
     2941 acagctccat gtacaacgcg atgctgttct tgaacggaag cgcgtcgatg acgtgccagc
     3001 ggttgttggt cacgaacccg cgcgtgcccg ggccgtcatt gcggggctgc ccgcagtagg
     3061 cgtggaagaa tatgtcggga gaagaccacg cgtaattgta gtagtcctct gagccggtac
     3121 cgaaggtcga aggccggaca tcgtcatcga cgaagatctt ctcgtcacct tcgccccacc
     3181 atccgccgcc cggcgttggg atctcggtcg ggttgagcag cagagacgct gttcccacgt
     3241 acacgccgcc gccgcgggca atcaagaacg gcagatcctg caccgcgtca ccggccccga
     3301 cgagatcgtg gttgacccgc cagcgggcgc ggaagtgcat cgaggtctta tcgttccact
     3361 cgtagtccat gggcaacact gagccgctga tcgatgccgc ctgctgtccg aggttttgga
     3421 ggacaatccg catggacttg gcaaagggca tcataaaccg gctggtcatg gtgccgtcgg
     3481 cctcaacggt gaaaggaacg gaatcgtatg ggttgattcc cggcccggcc ccgaagaagt
     3541 cacccaccgg tgattggacc tgtccccagg ggtagtcatc acagatgacg tgcatgactg
     3601 tttggcgcag agcgcggtcg atatccttgg cactcagctt cagcgtcagc cgctcgacgg
     3661 cctgggggcc tttcagcttg aggacctctc tcacctcgcc gggcgccaga tcggccttga
     3721 tctcgaccgg ggcggcggtc gaggcgtact tccagttggc cgccggggcc gccagcacct
     3781 tgccgatctc gcggatctcg ttggcgtact ttttcacgtc ctgcggaccg aaggtcacca
     3841 cgttggccgt cggttcgtac aggcgaatct gaatctgata gaagtggatc tcattcacgt
     3901 tgccgaccca gatgatccgg cattgtttgg cgaacggcat cggcgcgtag ccggcccgat
     3961 tctgctgaaa gctgttgcga tagaaatcag gatcgatgcc ggcttccttg ctgaacggct
     4021 cgtacggtcg atacagaaac tcctcggccg ggccgtcgaa gagaggcttg tccatgtcgt
     4081 ccaggaacag acggatgttg cccctgatcg cggccgtcca ggtgcggacg atcgccccgg
     4141 ggcccttcac gtcgcagatg aggtactcgc ccggcttgcc gtcggccggt tcacgaatga
     4201 ccgcctcgaa attcggcacc ggctcacggc caaagccatc ggagttggcg aaccactccg
     4261 gcccgcccgg caccttgctg cggtgatcgt aagatgagaa ctgcaccgtc ttgtacgccg
     4321 ggctcgggaa atcggccagt ttgtgaagat cgatcatctc ccggagcaat gaaccggtgg
     4381 tgatcgactc ggcctgagcc gcgctgacga gagctgcgct gatgaacagc cacatccaca
     4441 cgtatcgccg tgtcatatgc gttcctttcg cgattgtgag ccaaggacta cccgactgca
     4501 ttgctcgcgg ggcagagtct aacacggccg aaaggggaat gtaaaccgcc aagtcgccaa
     4561 gacgccaaga agaaaacgtg aaagcgtcga gtggtctgtt ttcagttctg cgccggtctt
     4621 cctgtgccag cgtcgttctg aaagtgcaga gatttcgccg gtttgaatat gtagtctt
//
LOCUS       DAOTPW010000341         4618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15297_length_4618_cov_5.696691, whole genome shotgun sequence.
ACCESSION   DAOTPW010000341 DAOTPW010000000
VERSION     DAOTPW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15297_length_4618_cov_5.696691"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1392)
                     /locus_tag="P5316_20410"
     CDS             complement(94..1392)
                     /locus_tag="P5316_20410"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRR87379.1"
                     /translation="MDFNKLGHTCVVGMHWGDEGKGKIVDLLTEHFDIVIRFNGGGNA
                     GHTVVVGTEKFALHLLPVGVLSPNKTAVVGPGVALDLAGIIDEIDKLAARGITIGDRL
                     RISDRANLVMPWHKKQDSLSEAALGDKKIGTTARGIGPCYADKMQRATAIRVIDLTHP
                     DQLRRKIERITQFKNTMFAALYNDREPLDAKAIAEQYLGFADRLRGNVTDTTMFLYDA
                     MQSGKRLLFEGANGTLLDIDHGTFPFVTSSSTSAIGVPAGAGVPAQTLKTCLGVTKAY
                     TTRVGAGPFPSELDNEIGQYIRDRGHEYGTTTGRPRRCGWFDAVAARYSARLSGITHV
                     AVMHLDTLTGLQQVGICTGYRTSAGTLPGLVADAVLMEQVEPVIEYMPGWKQDLREAR
                     SIGQLPAETRDYIDRLETLIGVPISIVSVGPERTQTLMRS"
     gene            complement(1709..2182)
                     /locus_tag="P5316_20415"
     CDS             complement(1709..2182)
                     /locus_tag="P5316_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87380.1"
                     /translation="MNKPPRLEPQDPITLFCPHCDYNLTGLPKNRCPECGRMFDPEQL
                     RMAQPLMDEDIRPITLLPAVILFFWPVVLVLPVSLLPHPSVWLLVVFAALIGNPVSMM
                     VLVRAVFARRARRAGLPFSIVRDFWPIAGLWVLLTALQYTGMILILLCIAATFRL"
     gene            complement(2226..2477)
                     /locus_tag="P5316_20420"
     CDS             complement(2226..2477)
                     /locus_tag="P5316_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRR87381.1"
                     /translation="MKLPRDLSGDDLTKALAVLGYQPTRQRGSHVRLTTQQGGEHHLT
                     VPQHSPLKLGTLAGILATVAAHFNLTRDQLMERLFGTKG"
     gene            complement(2474..2680)
                     /locus_tag="P5316_20425"
     CDS             complement(2474..2680)
                     /locus_tag="P5316_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HRR87382.1"
                     /translation="MNEVIFLVEEAPEGGYTARALGEAIFTEAETLDELRANVREAVE
                     CHFDADSRPKIIRLHLVREEVISI"
     gene            complement(2819..>4618)
                     /locus_tag="P5316_20430"
     CDS             complement(2819..>4618)
                     /locus_tag="P5316_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87383.1"
                     /translation="LSSLPIPNARHAPVQRQAAHRRLPAGLQDRASSIPHSQLAIRNS
                     PTLHVGNVGPYTHTMPIAVRGLTLGLDEPEDLLPDRAAKRLRIKPEDIRLWAIVRRSL
                     DARKHDRLGFVYNIELALSGSPRQEAQLVKRLRRADVALITSEEPPPLEPGRESLAGR
                     PVVVGFGPAGMFAALLLASHGYRPLVIDRGPDVKTRNQNVLVGFYREHRFNPESNLLF
                     GEGGAGTYSDGKLYTRVNDPRARMILETFYQHGADPDVLIDSRPHIGSDRLPAICRRI
                     RQRIEQLGGEVRFSSRLDDLVIRDGALSAIVVNGETIACGPVILAVGHSARDTLRMLG
                     RRGVAFEPKPFQLGVRIEHPQSMVDRWQYGPHCGHPRLPPAEYRLVAKGAAGDRGDVF
                     SFCMCPGGMILPTNESPGEISTNGASRSRRSGPLANSGLVVTVDPREVHPPVQNDPLA
                     TFDFLEQIERTAFEITGRSYKLPIQRACDFVQGHPSDGKLETSYPLGGQWSDLRQILP
                     DGIAQSIARGIEQLNRRLPGFGGPDALVAAPETRASGPVRMLRVPQTRESVSTRNLYP
                     VGEGAGYAGGIISAAVDGLKSAETIISRFAKPA"
BASE COUNT          785 a         1316 c         1640 g          877 t
ORIGIN      
        1 gccccggatc gagcgacaac gggtatctca ttctcaattc cacattcgcc attggccatt
       61 cctgggcgtt gcggcatgca acgctggggc ttgttacgac cgcatgagcg tctgggttcg
      121 ttccggcccg acgctgacga tggaaatcgg tacgccgatg agcgtctcga ggcggtcgat
      181 gtaatctcgg gtctcggccg ggagctgtcc gatcgaccga gcctcgcgca aatcctgttt
      241 ccagccgggc atgtactcga tgaccggctc gacctgctcc attaacacgg catcagccac
      301 gagaccgggg agcgtgccgg cggaggtccg atagccggtg cagataccga cctgttggag
      361 gccggtgagg gtgtcgagat gcatgaccgc gacgtgggtg attccgctga gtcgtgcgct
      421 gtatcgggcg gcgacggcgt cgaaccagcc acagcggcgc gggcggccgg tggtggtgcc
      481 gtactcgtgg ccgcggtcgc ggatgtactg accgatttcg ttgtcaagtt cgctggggaa
      541 cggcccggcg ccgacgcgtg tggtatatgc tttggtgacg cccaggcagg ttttaagcgt
      601 ttgggcgggc acgcccgcgc cggccggcac gccgatggcg ctggtggagc tactggtgac
      661 gaacgggaac gttccgtgat cgatgtccag tagcgtgccg ttggctccct cgaagagcaa
      721 tcgcttgccc gattgcatgg cgtcgtagag gaacatcgtg gtatcggtca cgttgccgcg
      781 caggcggtcg gcgaagccca ggtattgttc ggcgatggcc ttggcgtcga gcggctcccg
      841 gtcgttgtag agggcggcga acatggtgtt tttgaactga gtgatgcgtt cgatcttccg
      901 gcgaagctgg tcggggtggg tcagatcgat gacgcggatg gccgtggctc gctgcatctt
      961 atcggcgtaa caggggccga tgccacgggc ggtggtgccg attttcttgt cgccgagagc
     1021 cgcttccgag agactgtctt gcttcttgtg ccaaggcatg accaggttgg cccggtcgct
     1081 gatccgcagg cggtcgccga tggtgattcc tcgggcggcg agcttgtcga tttcgtcgat
     1141 gatgccggcc aggtcgagtg cgacgccggg cccgacgacg gccgttttgt tggggctgag
     1201 cacgccgacc gggagcaggt gcagggcgaa cttttcggtg ccgaccacaa cggtgtggcc
     1261 ggcgttgccg cctccgttga aacggatcac gatgtcaaag tgctcggtga gcaggtcgac
     1321 gattttgcct ttgccttcgt caccccagtg catgccgacg acgcacgtat gtcccagctt
     1381 gttgaaatcc atgtccttct cgcttcagta cttaagggcg gagacaaagc cccaggcgcc
     1441 agcgccggtc cggcgttttc ctgccatgga ggccggcgtg tcgccggcgc tccgggttgt
     1501 gttgaagacc ctcaggcccc ggggcgggcg caccatctgt gcgctcggtt ccggcggact
     1561 ctgattggtg gacgctttgt cataggttaa tggcgggggt tgggggcgtc aagccgtgca
     1621 gcagcagggg ggccgggggt gtcccgacgt tgccagtggg agggactctg tcgggatcgg
     1681 tattgattgc gccgtgagtt gaacgtcgtc agagacggaa tgtggcggcg atacacaaga
     1741 gaatcaggat catgccggta tactgcagag cggtcagcag tacccagagt ccagcaatcg
     1801 gccaaaagtc acggacgatg gagaacggca agccagcccg tctggcccga cgcgcgaaga
     1861 ctgctcgaac caggaccatc atgctgacgg ggttgccgat caaagccgcg aagacaacaa
     1921 gaagccacac ggaagggtgt ggcagaagag agacgggcag gaccaagacg accggccaga
     1981 agaacaggat gacggccggc aagagtgtga taggccggat gtcttcatcc atcaggggtt
     2041 gggccatccg tagttgttcc ggatcaaaca tccggccgca ttccgggcag cggtttttgg
     2101 gcaagccggt gaggttatag tcgcagtggg ggcagaagag ggtgatgggg tcctgtggtt
     2161 ccagtcgggg cggcttgttc atcccgcatc cgtttctccc ggtgggctga aggtcacagg
     2221 gcggttcatc ccttcgtccc gaagaggcgc tccatgagct gatcgcgcgt gaggttgaag
     2281 tgtgcggcaa cagtagccag gatgccggcc agcgttccca gtttcagcgg cgagtgttga
     2341 ggaaccgtaa ggtgatgctc gccgccctgt tgggtggtca accgaacgtg cgaacctctc
     2401 tgccgcgttg gctggtagcc caagacggca agggccttcg tcaggtcatc cccagaaaga
     2461 tcgcgtggaa gcttcatatg gagatgactt cctcgcgcac caggtgcagt cgaatgattt
     2521 tcgggcggct gtcggcgtcg aagtggcact caacggcctc gcggacattc gctcgcagtt
     2581 cgtcgagcgt ttcggcttcc gtgaagatgg cctcgcccaa ggcccgggcg gtgtatccgc
     2641 cttccggggc ttcctcgacc agaaagatga cttcattcat gctcgttcct cgcgtttcgt
     2701 ttcagccagt atcataccat gtttcgacgg cgtgtgaatc acgccaacca ggctactcgc
     2761 ccacgcagag ccgtggccat ggcacccggc acccggccgt gggcatggca cccgatcctt
     2821 atgctggttt ggcaaatcga ctgatgatcg tctccgcgga tttcagtcca tccaccgccg
     2881 cggagatgat gcctccggcg tagccggcgc cttcgccgac agggtaaagg ttcctggtgg
     2941 aaaccgattc gcgggtctgc gggacgcgga gcatccgcac ggggccgctg gcgcgggttt
     3001 cgggggcggc gacgagagca tcggggccgc cgaagccggg aagacggcga ttgagttgct
     3061 cgatgccgcg ggcaatggat tgggcgatcc catcaggaag gatttgccgc aggtcggacc
     3121 actggccgcc aagggggtag ctggtttcga gcttgccgtc ggatgggtgg ccctggacaa
     3181 aatcgcaggc tcgctggatg ggcagcttgt acgagcggcc ggtgatttcg aaggccgtcc
     3241 gctcgatctg ctcgaggaaa tcgaacgtgg ccagcggatc gttctgcacc ggcggatgga
     3301 cctcgcgagg gtcgacggtg acgaccaaac cgctgttggc caacggtccg ctgcgccttg
     3361 agcggcttgc gccgttggtg gagatttcgc cgggtgactc gttcgtgggc aggatcattc
     3421 cgcccgggca catgcagaag ctgaacacgt cgccgcgatc accggcggcg cccttggcga
     3481 cgaggcggta ttcggcgggc ggcaggcgag ggtggccgca gtgcgggccg tattgccagc
     3541 ggtcgaccat gctttgcggg tgctcgatgc ggacgccgag ctggaacggc ttgggctcga
     3601 aggccacacc gcgacggccg agcatgcgga gcgtgtcgcg ggcggagtgg ccgacggcca
     3661 ggatcaccgg cccgcaggcg attgtttcgc cgttgaccac gatggccgag agcgcgccgt
     3721 cgcggatgac gaggtcatcg agtcgcgatg agaaccgcac ctcaccgcca agttgctcga
     3781 ttcgctgtct gatccgtcgg cagatggccg ggagcctgtc tgagccgatg tgcggtcggc
     3841 tgtcgatgag aacgtccggg tcagcaccgt gctggtagaa cgtttcgagg atcattcggg
     3901 cgcgcgggtc attgacgcgc gtatagagct tgccgtcgga gtaagtgccg gccccgccct
     3961 cgccgaagag caggttggat tccgggttga atcggtgctc gcggtagaag ccgaccagga
     4021 cgttctgatt gcgggtcttg acgtccggcc cgcggtcgat gaccagcggg cgatagccgt
     4081 ggctggccag cagcagggcg gcgaacatgc ccgccggccc gaacccgaca accaccggac
     4141 ggccggccag ggactcgcgg ccgggctcca gcggcggcgg ttcctcggac gtgatcaagg
     4201 cgacatcagc acggcgcagc cgcttgacga gctgagcctc ttgtctgggc gagccgctca
     4261 gggccagctc gatgttatag acgaagccga gacgatcgtg cttgcgtgcg tccagcgacc
     4321 gccggacgat ggcccacagg cggatgtcct cgggcttgat gcgcaggcgt ttggccgccc
     4381 ggtcgggcag caggtcttcc ggctcatcga gccccagcgt caatcctcga acggcgatcg
     4441 gcatggtatg agtgtacggc ccgacgttgc cgacgtgcaa cgttggggaa ttgcgaattg
     4501 cgagttgaga atggggaatt gatgaagccc gatcctggag accagccggc aggcgtctat
     4561 gagcggcttg ccgctggacg ggcgcgtgac gggcgtttgg tataggcagc gatgacag
//
LOCUS       DAOTPW010000342         4610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15325_length_4610_cov_5.617344, whole genome shotgun sequence.
ACCESSION   DAOTPW010000342 DAOTPW010000000
VERSION     DAOTPW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15325_length_4610_cov_5.617344"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            627..3371
                     /locus_tag="P5316_20435"
     CDS             627..3371
                     /locus_tag="P5316_20435"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87384.1"
                     /translation="MAEMVSGNGRRRHRRTRRDSSSCTGNNAARALRPAADKVGGFLE
                     RGWVIANHKLVSFHAAFISSVLSVPEWAARKGDVLRFMFASTETLISLAILYLSWHIS
                     IAIHEMGHYLTAAKLTALNKSSQQYAEEMLKKSGVARAIWYLKMILLIPWGKFEGVKK
                     EEGNYVPDAPYNLAVAASAPVWSGWLAAICLPIAVICIFLGLISTPDNNVLIVLGRFF
                     LAPGVVGLLDRFLADRGKLKEFRMREQRAAEEAARAAVAITAESWMTKAKALKERMIT
                     TRTQTVTLADGSKVTTPWQWRNCGMGGLHTQKEYPESNISMQESMFIPLSPKTFEEAQ
                     EMTVRLQYRLKEVIESAPGAKVMGIGSEGGLSAYIDKEPTDRVPEQRLWRMMKQTILD
                     CGYVPGVDVAIALDPAASALEEAYREEKGEKDSVGMYRFWRDAHKVDMSRDEILELYR
                     DAMEKDGVPIISIEDGFGEKDHAGWKLIMEKMGDRIFIIGDDLVTTKDSTIEECAKNG
                     EINAALIKANQIGTLSETILAMLTATAYGAELIVSHRSKSPNDPFEADIATALDAVGM
                     KCGGGANTERLQKYGRVMEILAVAQSGKRQISEKERKEAELLLKELVATLTGRKDVEL
                     APGATDLDMVRLLLRVLAIEMVTGTEEATNTGIPSAAATVFLGKTGTLRFKGSTPLGA
                     STGSDEAIHYIDSMIVPCEVTRKHPSLFQKLDDGTLRFAKNVRLADIESKNDPELMAL
                     WKKVQRYDGKGCMEAVAHIENILGRAFVGRTLAQLGTLEEIDRELLALEKAEAIKLGW
                     LSPNAPVNDQIRVMQRKAAVGMNAILSMSIALGRVTAAKEGKELWQLIREVATSTMAK
                     FIAANAKDGTKDPAAIKSMPFEEIKALYRKTAEQVIKEGKAVHQLLRQQLPIYSDARG
                     "
     gene            4293..>4610
                     /locus_tag="P5316_20440"
     CDS             4293..>4610
                     /locus_tag="P5316_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87385.1"
                     /translation="MLKNGDNATDHNCTLDTTRPATRAGLRGNPQVSPAAASQGLDLS
                     RPIHGAGGIGGLLATEEPQGTYQGTYWFFYDAEPLTRRATRSVGAAGPRAKPARRVKG
                     NGNL"
BASE COUNT         1015 a         1458 c         1360 g          777 t
ORIGIN      
        1 aacgggctga gaccacagaa aggaagaaga accgcgggcg accaccaacc ggccgtacaa
       61 cggccggcct atcagcccat ctttggcggg aaaacccgct gaacggcctt gatcgcccaa
      121 cctgcaccgg tccgcatgcc cgcccagacg gtcacgcacc cccggacaat cgctacatct
      181 gccaccgctt gacatcctcc accccccgtg gtaggagatt acgcccgctt ggcaggtgtg
      241 gcgaggccgc gcggcacgcc ctggcgcact ccgggtatgc cctctgcccc gcggcagggc
      301 gtcgcgagaa acatccgcga tccccgcata gatgaatgga aagcgcatcg gttgtcgacc
      361 gggctgactg ctgatcggga aaggcacgtt gcgtacaggc aggagtcctc ccgagccaaa
      421 tgcatgcccg ccggcaaacc cgagtgcggt gagatgacgc tcgcccgggc agctttcggg
      481 ccgccttcag cacgatcagc caccgaacaa gccgtgctga aattggaaga gtggacaagc
      541 aagatcgaag tgtgtgacgg ttgctgaatc ctgacgtgac tgaccttcac aagacggtga
      601 acacaacggt gaaaggaaac attcaaatgg cagagatggt ttcagggaat gggagacgca
      661 gacatcgacg aacaagaaga gattccagct cctgcaccgg caacaacgca gcacgtgcat
      721 tacggccggc cgcggacaag gtgggcggat tcctggaaag aggctgggtc atcgcaaacc
      781 acaagctggt ctccttccat gccgccttca tttcgtccgt gctgagcgtg cctgagtggg
      841 cggctcgaaa aggcgacgtc ctcaggttca tgttcgccag cacagagaca ctcatctcgc
      901 tggccatcct gtacctgtct tggcatatca gcatcgcaat ccacgagatg ggtcactacc
      961 tgaccgccgc gaaactcacc gcgttgaaca agtcttcgca gcaatacgca gaagagatgc
     1021 tcaaaaagag cggcgtggca agagcgatct ggtatctcaa gatgattctg ctcatcccct
     1081 ggggcaagtt tgagggcgtc aagaaggaag agggcaacta tgtgcccgac gccccgtaca
     1141 acctggcggt tgcggcttcc gctcccgtct ggagcggctg gctggctgca atctgcctcc
     1201 cgatcgccgt catctgcatc ttcctgggct tgatatcgac ccccgacaac aacgtcttaa
     1261 tcgtcctcgg gcggttcttc ctcgcgcccg gcgtcgtcgg cttgctggac cgcttcctgg
     1321 ccgaccgcgg caaactcaag gaattcagga tgcgcgagca gcgcgccgcc gaagaagccg
     1381 cccgcgccgc cgtggccatc acggccgaga gctggatgac aaaggccaag gccctcaagg
     1441 aacgaatgat cacaacccgg acgcagaccg tcaccttggc cgatggaagc aaggtgacca
     1501 cgccctggca gtggcgcaac tgtggcatgg gcgggcttca tacccagaag gagtatcccg
     1561 aaagcaacat cagcatgcag gaaagcatgt ttatcccgct gtcgcccaag accttcgaag
     1621 aggcccagga aatgaccgtc aggcttcagt accgcctcaa ggaggtcatc gaatcggccc
     1681 ccggtgccaa agtcatgggt atcggctcgg agggcgggct gtcggcctac atcgacaagg
     1741 agccaaccga ccgggttccc gaacagcggc tgtggcgcat gatgaaacag accattctcg
     1801 actgcggcta cgtccccggc gtggatgtgg ccatcgccct cgaccccgcc gcttcggcgc
     1861 tggaagaggc ctatcgcgag gaaaaaggcg aaaaggactc cgtcggcatg tatcgcttct
     1921 ggcgcgacgc ccacaaggtc gacatgagcc gcgacgaaat cctggagctt taccgcgacg
     1981 ccatggaaaa ggacggcgtt ccgatcatct ccatcgagga cggctttggt gagaaagacc
     2041 acgccggatg gaaactgatc atggaaaaga tgggtgatcg gatcttcatc atcggcgacg
     2101 acctcgtgac caccaaggac agcaccattg aagagtgcgc aaagaacggc gagatcaacg
     2161 ccgccctgat caaggccaac cagatcggta cgctctcgga aacgatcctg gccatgttga
     2221 ccgctacggc ctatggcgcc gaactgatcg tctcacatcg gtccaagtcc ccgaacgacc
     2281 cgttcgaggc cgacatcgca acagcattgg atgccgtcgg catgaagtgc ggtggcggtg
     2341 ccaacaccga gcgcctccag aaatatggac gcgtcatgga aatcctcgcc gtggcccagt
     2401 ccggtaaacg gcagatctcg gagaaggaac gtaaggaggc tgaattgctt ctcaaggagt
     2461 tggtcgccac gctcaccggc cgcaaagatg tcgagctggc ccccggggcg accgaccttg
     2521 atatggtcag actcctgctc cgcgtgctgg ctatcgaaat ggttacagga accgaagagg
     2581 ccaccaacac cggtataccg tcggcggccg cgaccgtctt cctcggcaag accggcacgc
     2641 ttcgcttcaa gggatcgact ccgctcgggg cctcgaccgg ctcggacgag gcgatccatt
     2701 acatcgacag catgatcgtt ccttgcgaag tcacgagaaa acacccctcg ctgttccaga
     2761 agttggatga tggcacgctc cgattcgcca agaacgtcag gctcgccgat atcgagtcca
     2821 agaacgatcc ggagctgatg gcgctctgga agaaggtcca gcgatacgac ggcaagggct
     2881 gtatggaggc cgttgcccac atcgagaata tcctcggacg agcctttgtc ggccgaacgc
     2941 ttgctcaact gggtacgctt gaggagatcg accgcgaact tctggccttg gagaaggctg
     3001 aggccatcaa gctcggctgg ctgtcaccga acgcacctgt caacgaccag atacgcgtca
     3061 tgcagaggaa ggccgccgtg ggcatgaatg ccatcctttc catgtccatc gccctcggcc
     3121 gcgtcaccgc agccaaggaa ggaaaggaac tctggcagct cattcgcgag gtggcaacca
     3181 gtaccatggc caagttcatc gcggccaacg ctaaggacgg caccaaggac ccggcggcca
     3241 tcaagagcat gcccttcgag gagatcaagg ccctgtaccg caagacggcc gagcaggtca
     3301 tcaaagaagg caaggcagta caccaactgc tgcgccagca gttgccgatc tactcagacg
     3361 ctcgcggctg acgtctgtcg ctgcggcgcg tcgatccttg agataaaaga ttcccagggc
     3421 ggttcgacga ttcgacatgg cgggccgccc tggtttcata aaccggcaag ctaagacgaa
     3481 ccgccttttt gcgggccgca actcaagcgt actgcgatcc agaaacacgc gcaagccgca
     3541 cacatgtctg gccgtagggt tccagcgctc aggaccaaaa cccgttgcat ggatatcggc
     3601 ttgccattcg atgcctaagg cttgtgacat tccgcaaccg gttcgaccgc accgcaagca
     3661 agccttcatc acccgcccct gtggagaatc tcggccagac aagccgatga gcgcgaaagg
     3721 acagaccctt cccgtgaatc ccatcttgtt tttcatcccc gcgatttgac aagatcgccc
     3781 gcgctgcgcg gggcatgcgc acgggctgcc tgcggggtct ggccacggtg cgatggcggc
     3841 gggtacgtaa ccgtccgggc gggcatgccg accggtccag gttgggcgac caaccccctt
     3901 cagcgggttt tcccgcccaa ggcgggctga taggccggcc ctcatacggc cggtcggtgg
     3961 tcgcctgcgg ttcttcttcc tttctgtggt ctcagcccgt ttcaacgggc ttgcctgaca
     4021 ggcccgttaa aacgggccga tggggcaaca cagagaagga tggttggccg acggtgaacc
     4081 agtcgtaaaa cggccggctt gtttgccctt cccgaaagac cgctgaagcg gtctgctttc
     4141 ggcttgcgtt caccacaacc gggtgagccg ccagaagtgc cgccgcttct ctcacggccc
     4201 cccaatggac cccaggtttc aaatgacttg acaactagtc atccatactg tacattttca
     4261 tcacccgcga caaatagcga ccggagcgct tcatgctcaa aaacggcgac aacgcaaccg
     4321 atcacaattg cacccttgac accacgcggc ccgcaacgcg ggccggcttg cgcggcaacc
     4381 cgcaggtttc acctgccgcg gcgagtcaag gcctggacct gtcccgcccc atccacggcg
     4441 ccggcggcat cggcggcctg ctggccacag aggagccgca aggcacgtac caggggacct
     4501 actggttctt ctacgacgcc gaacccttga ctcgccgggc aacgcgaagc gttggggccg
     4561 ctggcccgcg ggccaagccc gcccgtcgtg tcaagggaaa cggcaacctg
//
LOCUS       DAOTPW010000343         4606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15337_length_4606_cov_5.913865, whole genome shotgun sequence.
ACCESSION   DAOTPW010000343 DAOTPW010000000
VERSION     DAOTPW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15337_length_4606_cov_5.913865"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..2928
                     /locus_tag="P5316_20445"
     CDS             301..2928
                     /locus_tag="P5316_20445"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRR87386.1"
                     /translation="MLFRFAILIVTAALSAGAASQSKPATLPVASRPATAPASRPVSR
                     SLVMSGVVFEDLDADGVRDDNEPALKDMSVSDGLGFVRTQADGRFEFKLREQSRGSVF
                     VCTPAGWRASKHFFVIADFDRYADKVQPADIGLVRDPARNTDRFVFVQLSDTHVTEAE
                     SAVQTMAEDLEVVNNLSDTPTFVVTTGDLTNVGKQVAQFEGYARATRDNRYPFYNVVG
                     NHDYGGELRDTENYEKYLGPPYYSFDVGPYHFIARDIIARDRKSGFFERQTEWIERDL
                     RLNAVGKRVVVFQHYIPTIRELDWWAERNGAAIFSGHWHGRREREYKGILDINSSTLR
                     FGGIDRSPRGFRIIHVDGQQMRCEWRLGAQRQRVEIVHPPVNGLTFGRAVPIRILAYD
                     TAVRVRGVRYRVFDADDAASNRTEPIARGDLWAEGSWTWAGRWQVPSDVWSGLKRIEV
                     EATAADGTVWKQAGVFKLDVNGVAIVKPGQAWPFFHNDAGHRGYVSDGPRPPLVPAWS
                     THIGGTIHISSPVIADGRVYAASSFESSLDDCAITALDLVTGRKLWRAPVDSSIKHSL
                     AVWDGNIMAVSQAATLYCFNKDGFPRWTASLDRGHDDRWETSFPVTDGKLVYAGRCSG
                     FGAYDLASGEPLWKQPGGSDWWPNIYSGPSLGTGVVYQGGPFARALDPATGDIRWSNN
                     QMAVSTVAVVPAAVEQSQDGDRLYVFQNKKTLLCLDGRSGQQLWEAAFESADGQGREV
                     VPLGDETGTPAIGESIVCVGSAEVSSKDQSFDAAMNGFDKATGELRWRFAVGQELSPT
                     VPYKRDSATITSSPVVVGDVVYFGASDGWFYALDVSDGHLLWKYRFGLPIASTAAVSG
                     NTVIVTTWDGTVHAMVATD"
     gene            2950..4383
                     /locus_tag="P5316_20450"
     CDS             2950..4383
                     /locus_tag="P5316_20450"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR87387.1"
                     /translation="MRCALLAVTAVFLLCSSIAIGEESATPPNVIFILADDLGWGDLS
                     CYGHQRLKTPHLDKLASQGTLFTQFYVNGSVCSPSRCAFFTGQYPARHRIHGHYAEHD
                     LNAQRGMSNWLDPKVPNVAALLKSRGYATAHFGKWHLGSGPGAPNPTEYGFDEAKTMV
                     SAFPAWHEPAATFWAKSTGLFVDEALRFIEANKSKPFYVNIWTLVPHATLNPTDEQMK
                     PYEHLGPKGVPHKGAEAIYYGSVGDLDAQIGRLLGELETMGVAENTLVVFSSDNGPED
                     IHITNAGHSGVGSAGPFRGRKRSLYEGGIRVPFIVRWPGHVPAGRIDNESVVAGVDFL
                     PTVCKLIGVQPPAGHALDGEDVSDVLLGQPRPRKTPLMWEWRFHVFGEPFHRSPILAI
                     REGDWKLLMNPDRSRVELYDIPRDPMQMNNLAEKNKDIVDRLAAKVLAWQKALPEGPI
                     EPTAGKDDYPWPGQAKPAPRQPRAKAR"
BASE COUNT          786 a         1393 c         1532 g          895 t
ORIGIN      
        1 ctccgaacat gcttacgggg atggttgcgg caggtctgcg cggcctttgg ccgctgcgac
       61 cgtgccctac gtgatctggc cgctccgaac atgccttacg gggatggttg cggcaggtct
      121 gcgcggcctt tggccgctgc gaccgtgccc tactgatctg gccgctccga acatgcccta
      181 ccttcatcac acgagccccg cgtgttgcgg tttacacccc ggtgcctatc attggttgtg
      241 atcccacgga tcggcgcgtc tgagagtacg tgttgttgag ctttcttttg gagatctggc
      301 ttgctgtttc ggtttgcgat tctgatcgtg acggcggcac tctcggccgg agcggcctcg
      361 cagtccaagc cggccacgtt gcccgtcgcc tccagacccg ccacggcccc ggccagccga
      421 ccggtctcgc gcagcctggt catgagcggc gtggtgttcg aggacctcga cgccgacggc
      481 gtgcgagacg acaacgagcc ggcgctgaag gacatgtccg tgtccgatgg acttggtttc
      541 gtccggaccc aagccgacgg ccgtttcgag ttcaaactcc gcgagcagtc tcggggctcg
      601 gtcttcgttt gcacaccggc cggttggcgg gcctcgaagc acttcttcgt cattgcggat
      661 ttcgatcgtt atgccgacaa ggtccagccg gccgacatcg gcctggttcg cgatcccgcc
      721 cgcaacaccg accgtttcgt cttcgtccaa ctgagcgaca cgcacgtcac cgaagccgag
      781 agtgccgttc agaccatggc cgaagacttg gaggtagtaa acaacctctc ggacacgccc
      841 acgttcgtgg tgaccacggg cgacttgacc aacgttggca agcaagtcgc gcagttcgag
      901 ggctacgcga gagccacccg agacaaccga tacccgttct ataacgtcgt cggaaaccac
      961 gactacggcg gcgagctccg cgacacggag aattacgaga agtacctcgg tccgccctat
     1021 tactctttcg acgtcgggcc gtaccacttc atcgcccgcg acattattgc ccgtgacaga
     1081 aagagcggct tctttgagcg ccagaccgag tggatcgagc gcgacctccg tctgaacgcc
     1141 gtcggcaagc gggtggtggt gttccagcac tacatcccga cgattcgcga actcgactgg
     1201 tgggcggagc gcaatggggc agcaatcttt tcaggccact ggcacggccg tcgggagcga
     1261 gaatacaagg ggattctcga catcaacagt tcgacgcttc gcttcggcgg catcgaccgc
     1321 agcccgcgcg gattccggat catccatgtg gatgggcaac agatgcgatg cgagtggcgg
     1381 ctgggtgccc agagacagcg ggtcgagatc gtgcatccgc cggtcaatgg cttgaccttc
     1441 gggcgtgccg tgccgatccg cattcttgca tatgacacgg cggtccgcgt caggggcgtt
     1501 cgatatcgcg tttttgacgc ggatgatgcc gcatccaacc ggaccgagcc gatcgcccgg
     1561 ggtgatctgt gggccgaggg ttcgtggacc tgggccggcc gatggcaggt tccgtcggac
     1621 gtctggtccg gcctgaaacg catcgaggtc gaggctacgg cggccgacgg caccgtttgg
     1681 aagcaggcgg gcgtgttcaa gctggacgtc aacggtgtcg caatcgtcaa acccggccag
     1741 gcctggcctt tcttccacaa cgacgcgggc catcgcggct acgtttcgga cggtcctcgc
     1801 ccgccgctgg taccggcatg gtcgacgcat atcggcggca cgattcacat ctccagcccg
     1861 gtgatcgccg acgggcgcgt ctatgccgcc tccagcttcg agagctcgct cgatgattgt
     1921 gccatcacgg cgcttgacct ggtgaccggc cggaagctct ggcgtgctcc tgtggactcc
     1981 tcgatcaagc acagcctggc ggtgtgggac ggcaacatca tggccgtgtc ccaggcggcg
     2041 acgctctact gtttcaacaa ggacggcttc ccgcgatgga cggcctcgct cgatcgcggc
     2101 cacgacgatc gttgggaaac gagtttcccg gtcaccgacg gcaagctcgt gtatgccggg
     2161 cgttgcagcg gtttcggcgc gtacgacctc gcgtcaggcg aaccgttgtg gaagcaaccc
     2221 ggcgggagcg actggtggcc gaacatctat tccggaccga gtctgggcac gggtgttgtc
     2281 taccagggcg gcccattcgc cagagcactg gacccggcca cgggggacat ccgctggtcg
     2341 aacaaccaga tggccgtctc gaccgtggcg gtggtcccgg ccgcggttga gcagagccag
     2401 gacggcgatc ggctgtacgt attccagaac aagaagacgc tcctgtgctt ggatggcaga
     2461 tcggggcagc agctctggga ggccgctttt gaaagcgccg acggccaggg cagggaggtt
     2521 gtaccgctgg gcgacgagac aggcacgcct gcgatcggtg agagcatcgt ctgcgtgggc
     2581 agcgcggagg tgtcgagcaa ggatcagtcg tttgacgccg cgatgaacgg cttcgacaag
     2641 gccaccggcg agttgcggtg gcgattcgcc gtggggcagg agctttctcc gacggttcca
     2701 tacaagcgag acagtgcaac catcaccagt tcgccggtcg tcgtgggcga tgtggtctac
     2761 ttcggggcga gcgacggctg gttctacgcc ctcgatgtca gtgatggtca tctgctctgg
     2821 aagtatcggt tcggcctgcc gatcgcctcg accgcggcgg tttccgggaa taccgtgatc
     2881 gtgaccacgt gggatgggac ggttcatgcc atggtggcca cggattagcc acactgggga
     2941 ggtcccgaca tgcgatgtgc tttgcttgcc gttactgcgg ttttcttgct gtgctcatcg
     3001 atcgcgatcg gtgaagaatc tgcaacgccg cctaacgtca tcttcattct cgccgacgat
     3061 ctgggctggg gcgacttgag ctgctacggc catcagcggc tcaagacgcc gcatctggac
     3121 aagcttgcct cgcaggggac gttgttcact cagttctatg tgaatggctc ggtttgttcg
     3181 ccgagccgct gcgcgttttt caccggccag tatccggcgc ggcatcgcat tcacggtcat
     3241 tacgctgagc acgacctgaa tgcgcagcgt ggcatgtcta actggcttga cccgaaggtt
     3301 ccgaatgtgg ccgcgctgct gaaatcgcgg ggctatgcga ccgctcattt cggcaaatgg
     3361 cacctgggta gcgggccggg ggcgccgaac ccgaccgagt acggttttga tgaagccaag
     3421 accatggtgt cggccttccc ggcctggcat gagcctgcgg ccacgttctg ggcgaagtcg
     3481 acggggctgt ttgtcgatga ggccttgcga ttcattgagg cgaataagag caagccgttt
     3541 tacgtgaaca tctggacgtt ggtgccgcat gcgacgttga acccgaccga cgagcagatg
     3601 aaaccatatg agcacttggg gccgaaaggc gtgccacaca agggtgccga ggccatctat
     3661 tatggctcgg tgggcgacct ggacgcacag atcggtcgct tgctgggcga gttggagacg
     3721 atgggggtgg ccgagaacac gctggtcgtt ttctccagcg acaacgggcc cgaggacatc
     3781 cacatcacca atgccggtca cagcggtgtc ggttcggccg ggccgttccg cgggcgcaaa
     3841 cgcagcctgt acgagggcgg cattcgagtg ccgttcatcg ttcggtggcc ggggcatgtg
     3901 ccggccgggc ggatcgacaa cgagtcggtc gtcgcaggcg tcgatttcct gccgacggtg
     3961 tgcaagctga tcggtgtcca gcctccggcg ggccatgcgc tggacggcga ggatgtcagc
     4021 gacgtcttgc tgggtcagcc gaggccacgg aagacgccgc tgatgtggga gtggcggttt
     4081 cacgtgtttg gcgagccgtt ccaccgcagc ccgattctgg ccattcgcga gggtgactgg
     4141 aagctgctga tgaacccgga tcgcagccgc gtggagttgt atgacatccc gcgcgacccg
     4201 atgcagatga acaacctggc cgagaagaac aaagatatcg tcgatcgatt ggctgccaag
     4261 gtcctggcct ggcagaaggc gctgcccgaa ggccccatcg agcccacggc cggcaaagac
     4321 gactaccctt ggccgggaca ggccaagccc gcgccgcgcc agccgcgagc caaagcgagg
     4381 tagggcagcg tcgaggtcgc cgcagcgacc gagacctgcc gtttctttct gtcgttggtt
     4441 ctgggcgtcc gttgaccagg ttgtcccggt cggggatggt tgcggcaggt ctgcgcggcc
     4501 tttggccgct tcgacctgcc ctacgtgatc tggcggctgc gaacatgctt acggggatgg
     4561 ttgcggcagg tctgcgcggc ctttggccgc ttcgaccgtg ccctac
//
LOCUS       DAOTPW010000344         4604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15348_length_4604_cov_7.232579, whole genome shotgun sequence.
ACCESSION   DAOTPW010000344 DAOTPW010000000
VERSION     DAOTPW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15348_length_4604_cov_7.232579"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..933)
                     /locus_tag="P5316_20455"
     CDS             complement(313..933)
                     /locus_tag="P5316_20455"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRR87388.1"
                     /translation="MAHVLPDLPYDFAALEPYIDEQTMRLHHGKHHAAYVNNLNGALA
                     AYPELAAKTIEHLLTKIDAVPADIRQTVINNGGGHANHDLFWTIMAPPGKGGGGEPAG
                     KLAEAIQSTFGGFAKLKETLNKAATSRFGSGWAWLVVDDKRKLQVYSTANQDSPLMQG
                     HTPILGLDVWEHAYYLKYQNRRPDYIDAWWNVVKWPAVAERFAKAS"
     gene            complement(1278..2444)
                     /locus_tag="P5316_20460"
     CDS             complement(1278..2444)
                     /locus_tag="P5316_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87389.1"
                     /translation="MTIRYECRCGANIRMPVSAAGRKARCQACGAVFTVPRPDYDPDT
                     QPIPLEGSPPQPPPTQDQEPSIGGWLADFARQEKSTAGDKPVVIESIASKSLSQELDR
                     EQAAREQARTSIPRISGRLPHEEARLHDAERSGVIAPEVPFWEDLLKSFLFPLDASNL
                     VTYLILGIAPFVCWWIPIAGPFLVLVVHLYLAAFCMEVIRETAAGEDRLPETVWMSSA
                     IDLLSPMFQFLGATALAMLPAAIYVLATIEPGSTLELIVWHFVRAPQMPAVQILAAVG
                     LLFWPIIVLAVAIGGSFSGLWPHVVARTALCAPAPYLAMCGTLLIAAVVWVLPSTSLF
                     DNLYIKAAQLLGRRGPLVIQLPQDLISLYGMIVAMRTIGLYYRHFKHKFPWAAE"
     gene            complement(2601..3578)
                     /locus_tag="P5316_20465"
     CDS             complement(2601..3578)
                     /locus_tag="P5316_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87390.1"
                     /translation="MLFIVFASMLTSAMAGTGERGVLSAKGTRSKLGLLYLDGGQPSA
                     HLLNLVDGGNVPIMKIPAGDFARPDSVGLRLKKANPKAIIVVGVGAQSKEMKIDRDPL
                     QVYANWAKEKEGDLRTLAAHKDKVDFLSFMPNMWEPKTVEQAQWYCRYIAHAAPRIAA
                     MGFRPIILQSGVGGLPIEPQILNAMVPALRIAKKHGGAWACHGYTLEYDTNVNRESYY
                     SLRYRRAYEYLKKTHPDVADLPMILMEGGVDKAGDPNKDGWQARGNADKYVAWLQWFD
                     SQLLEDPQVLGVCLFKIGNVNEWRSFDLEPVAPWLGEYLKKKNTQTKPR"
BASE COUNT          853 a         1399 c         1415 g          937 t
ORIGIN      
        1 cggactcgcc tgctgtcaac agcacgagac cctccgccgg ttgcccatag gtttatggcg
       61 tcgcaggggg ctttttcggc ggcggggtgg gtaccggaga accgtcgagc atctgattct
      121 tgacaagggg gtgcaaccgg cagcttgtgt gccggcgtgc ggtccagacg aggatgctga
      181 gaaggcggaa aaaagaccgg ccgtcgctct ccccgggcgg gggaaagtta ggccggttgg
      241 actgacagag gggtgggtgt tgtgatcggt tcggtcctct gccagccctg cttggcaagg
      301 agtgaagcct tgtcaggatg ccttcgcaaa acgctcagcc acggccggcc attttaccac
      361 gttccaccac gcgtcaatat agtccggccg acgattctgg tatttcaaat aatacgcgtg
      421 ttcccacacg tcaagaccga gaatcggggt atgcccctgc atgaggggcg aatcctggtt
      481 agccgtcgag tagacctgca actttcgctt gtcatccaca acgagccaag cccatcctga
      541 accgaaacga gaggttgccg ccttgttcag tgtctctttg agcttggcga atccgccgaa
      601 ggtgctctgg atggcctccg ccagcttacc ggcgggttcg ccacccccgc ccttgccggg
      661 cggggccatg atcgtccaga acaagtcgtg attcgcgtgc ccacccccgt tgttgatcac
      721 cgtctggcgt atgtccgccg ggaccgcgtc gatcttggtc agcaggtgct cgattgtctt
      781 ggctgccagc tcggggtagg cggccagcgc gccgttcagg ttgttcacat aggccgcgtg
      841 atgcttgccg tggtgcagtc gcatggtctg ctcatcaatg tagggctcca gggccgcgaa
      901 gtcgtagggg aggtcaggta gtacgtgggc catcgctttt gatcctttcg tggagtcgtg
      961 agcaggagag gacggccgcg acgggttgtt ttccctattc cgatgatctg ccgcgtggct
     1021 attgcgggga ccccaaatga tcgccccgcc cagtgctcct gctttcagta tcaggtcacg
     1081 gcgtctcatc tccggctcga ctcctttctg ctgccaggaa tccggccgct tcggcggccg
     1141 cttccgaacg tttgtcactt gttcagccgg attctagtag gccctcctgt gctcgaccac
     1201 aagatttcgc cggcaaacgc acgttgccaa atggttgtcg agaagaagat caggtggtcg
     1261 ttgtgctccg agggatgcta ttcagcggcc catgggaact tgtgcttgaa gtgcctgtag
     1321 tacagaccga tggtccgcat agcgacgatc attccgtaga ggctgatcag atcttgtggg
     1381 agttgaatca ccaggggacc gcggcggccc agaagctgcg ctgctttgat gtacaggttg
     1441 tcgaacagcg aagtgctcgg caggacccag actactgcgg caatcagaag tgttccgcac
     1501 atggccaagt acggagccgg ggcgcataag gccgttcgtg cgacaacatg aggccacagc
     1561 ccgctgaagc tgcccccgat ggccacggcc aggacgatga tcggccagaa aagcaatccg
     1621 actgcggcca ggatttgcac cgccggcatc tgcggggcgc ggacaaagtg ccagacaatc
     1681 agctccaggg tgctgcccgg ctcgatggta gcaaggacat agatggcggc gggcagcatc
     1741 gccagtgccg tggcgcccag gaattgaaac atcggcgaga gcaggtcaat cgcgctgctc
     1801 atccacaccg tttccggcaa ccggtcctcc cctgccgcgg tctcccggat gacctccatg
     1861 cagaacgccg cgaggtagag gtgcacgacg agcaccagga acggtccggc gatcgggatc
     1921 caccagcata cgaaaggggc aatccccaga atcaggtagg ttaccaggtt cgatgcgtcc
     1981 agcggaaaca ggaacgactt gagcaggtcc tcccagaaag gcacctcggg tgcgatgacg
     2041 cccgaccgct cggcgtcgtg gagccgggcc tcctcgtgcg gcagacgccc gctgattcgc
     2101 gggatggacg ttctggcctg ctcccgggca gcctgctcgc gatccaattc ttgagaaagg
     2161 cttttagagg ctatagactc tattacgacc ggtttgtcgc cggcggtgct tttctcctgg
     2221 cgggcaaagt cggcgagcca gccaccgatg gacggctcct ggtcttgcgt cggcggcggt
     2281 tgagggggac tgccttccag ggggatgggt tgggtgtccg gatcgtaatc cggccggggc
     2341 accgtgaaga ccgccccgca ggcctggcag cgagccttcc ggcccgccgc cgagacgggc
     2401 atccggatgt tcgccccgca ccggcattca tatctgatgg tcatcgccgg ttctccgtat
     2461 cgcggaacaa tcccgtgaag acgtatgacg cttctccgaa ccggatgtat gtgtcctcca
     2521 gttgcatcag accccgcaga caacattctg ccggtgctca tgaggcgggc accctatccg
     2581 actctcttgt tcgctgacac tcagcgaggt tttgtttggg tattcttctt cttcaggtac
     2641 tctcccagcc aaggggcaac cggttccagg tcgaaggacc tccattcgtt cacgttgccg
     2701 atcttgaaga ggcagacccc caggacctgc ggatcctcaa gcaattgtga gtcaaaccac
     2761 tgcagccacg ccacgtattt gtccgcgttg ccgcgggcct gccaaccgtc cttgttcgga
     2821 tcgccggcct tgtccacacc gccttccatc aggatcatcg gcaggtccgc cacgtcggga
     2881 tgcgttttct tcaggtattc gtacgcccgg cggtaccgca ggctgtagta gctctcgcgg
     2941 ttcacgttgg tgtcgtactc aagggtgtat ccgtgacagg cccatgcacc gccatgcttc
     3001 ttcgcgatcc gcagcgccgg caccattgcg ttgaggatct gcggctcgat cggcagccct
     3061 cccacgccgc tctgcaggat gatcgggcga aaacccatcg cggcgattct cggcgcggcg
     3121 tgggcgatgt accggcaata ccattgcgcc tgttccaccg tctttggttc ccacatgttc
     3181 ggcatgaacg agaggaagtc caccttgtcc ttgtgcgccg ccagcgtacg caggtcgccc
     3241 tctttctctt tcgcccagtt cgcgtaaacc tgaagcggat cacggtcgat cttcatctct
     3301 ttcgactgag cgccgactcc gaccacgatg atcgccttgg gattggcctt tttgagacga
     3361 aggcccacgc tgtcgggtct ggcgaagtcg ccggcgggga tcttcatgat cgggacattg
     3421 ccgccgtcca ccaggttcag cagatgcgcg gacggttgcc cgccgtccag atagagcagg
     3481 ccgagtttcg acctcgtacc ttttgcagac agcacccctc gctcacccgt ccccgccatc
     3541 gcggacgtaa gcatcgaggc gaacacaatg aacaacatat gcttttcaaa acgcaattga
     3601 ggcacctcca acatcatgac tggctcacag tcctaacgaa aacacgtaga cgctctgccc
     3661 gattgtgaac ctgattctgc gcgggccatc ccggctcgca gcggctttga cccgcgttca
     3721 tgccctgcct tcccttcatc gaacgtctcc agcctgtgcg gcgagcggct ctgattgcca
     3781 ccgagcatct cggcgatcag atggcagtga ttgggcatcg ggcagcaggc gtgtgccgcc
     3841 aaccggaaac ggttccaccc gctacacggt ccccaagaga agcgcccttt tggggggccg
     3901 caacccaaac gcacagtgct ccgaaaacat gcgcgcaagc cgcacagata tctggccgta
     3961 aggttgtgat gaaccggcga cggttctcat cgtcgcgaac tcctgttctg cgctcattgt
     4021 cccggacagt tacatgatac accaccgctg gacattcagt ccgaaatggg cttggccagg
     4081 cctcgcatgg tatctgatcc gcgatggcca acatgccagg aatcgagaga tgagccctgc
     4141 ccttgggagt cgaccccagc ccggcaagaa tcgttgagcc aagtctgacg agggttccgc
     4201 tggaataacg ggcatgagcc gatggggaaa ggcatcgcgg gggtgggtta aagcagcgag
     4261 gagaaacgtc cgttattcgc gtaaacactt ctgctacagg cgcttagcag tttttgggtt
     4321 gggttcgttt ggcgcggggg ttggtcaggg cggcggcagg tcggcgccct gcttcgtcgg
     4381 ggcgacttgg tggggctgtg acctgcccta cctgcttcag gattcatttg gaggcgcatg
     4441 cccacgcaaa gccgtgggca tggtgcccga cggacctgcc ctacctgctg aatgcggaaa
     4501 ggattccgca ggggatgcgg cccggaaagg attccgggcc gagcggatac atttggaggc
     4561 gcatgcccac gcaaagccgt gggcatggca cccgacggac ctgc
//
LOCUS       DAOTPW010000345         4557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15525_length_4557_cov_5.985118, whole genome shotgun sequence.
ACCESSION   DAOTPW010000345 DAOTPW010000000
VERSION     DAOTPW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15525_length_4557_cov_5.985118"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="P5316_20470"
     CDS             complement(<1..621)
                     /locus_tag="P5316_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87391.1"
                     /translation="MQRVAFRSCPAVPVELPVPPGVAGAIRRARCGRRGVMLLEVVVA
                     LGLLVFGLAVVGLQIDTGLKIAKTNRLYTQAMMLVDSKLSEVEAGVIQPDMLEREVKG
                     DFGIIYPGFTWRIEIEPTEIEGFYMAKIEIGYCENAVEAQINDPMMEIDIEDPDTVIV
                     RTAYRLFPEPADINLERDYGLTQEAMDALLADLEGLDPMGGGGGGGG"
     gene            complement(737..1495)
                     /locus_tag="P5316_20475"
     CDS             complement(737..1495)
                     /locus_tag="P5316_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR87392.1"
                     /translation="MRLRAFTLLEVLLVIALMGLLMALVYPSFESDRRHRSLTESAER
                     LRALIEMAHARAMQEGIRYRISFPGTPDPNDPNAETEVDVPFETLQPEVVRQIDPQGS
                     PEAYGGFDADWKNQPILQAGTRCVSVLPGKPSYAINTRWPIADPGMPEDKTQFVPLTL
                     FPDGTTEWVTFILTDLPFDTELEPHHFTRILYLIVDGRTGQVWMQRAMLNDEVEIMHE
                     YKAEPIMHVDFVSPEVITEANILDIRRTRAVGSR"
     gene            complement(1622..2068)
                     /gene="gspG"
                     /locus_tag="P5316_20480"
     CDS             complement(1622..2068)
                     /gene="gspG"
                     /locus_tag="P5316_20480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRR87393.1"
                     /translation="MLQVKRVRNRRRGFTLLEILVVVGILALLAAFVVPSLIGTQRDA
                     YIKTTKANMDSLASVMQLFNLHMGRFPKELAELRDKPEDDAEAEKWKGPYIDSLDKLK
                     DAWGHEFQYLGNDEAKANEGSYDIWSLGPDGEDGTDDDICNWTKDK"
     gene            complement(2182..3387)
                     /locus_tag="P5316_20485"
     CDS             complement(2182..3387)
                     /locus_tag="P5316_20485"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRR87394.1"
                     /translation="MGTFTYKAKTTGGQTVTGVLTAESQQAAMRMLDERSLFPIKVAE
                     GGTAARATITGRRKKVRLRVLTTFYSQLSDLLRAGVPVLRALDVLSRQGANPLLAEIL
                     KEVHTDVSSGESLAEAMSKHPNAFTHLAVSMVHAGEQGGFLEDVLARIAVFTERQDEL
                     RNKVIGAMIYPCVLMLAGVSVVTFLMGFVVPKIKPLLERQQVPWLTTVLFAVGDFVAN
                     HGWVILGVVVALVVLAVGLTRREEARRLLDRWKLRVPVLGRILTMVAICRFCRILGTL
                     LGNGVTILQALKISKDSAGNRILAEAIDQAAENVQRGESLAARLGRSGLFPADIVDMI
                     AVAEESNNLDAVLVQIADSNEARTSRLVDFGVRLLEPLLLMVMAAMVLVIAIALLVPI
                     LRMGSAGLG"
     gene            complement(3786..>4557)
                     /locus_tag="P5316_20490"
     CDS             complement(3786..>4557)
                     /locus_tag="P5316_20490"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRR87395.1"
                     /translation="FPDGLKEPRAAILCSHGHGIGMNELVALTEKGEPRQYKEGYQHD
                     FAIQAVRAGFVTLVFDQMGFGRRRDIDFHKQQNMWNHCEQPSKNALHWGLSMTGIRVW
                     DAMRMIDFLQSRPEVDPKRIGMVGISGGGLVTQFTAALDDRIRAACVSGYLNRYETCI
                     LSIHHCIDNYVPGIGLQANNDDLACLIAPKPLLIEAGRKDPIFPIAATEEALRKLQRC
                     YRVAGAPEALETDVFDGPHEFSGAKTWDFFARHLKQSE"
BASE COUNT          912 a         1530 c         1239 g          876 t
ORIGIN      
        1 cccgccgcca cccccgccgc cacccatcgg atcgaggccc tcaagatcgg ccagcagtgc
       61 gtccatggcc tcctgggtca acccgtagtc ccgctccagg ttgatgtccg cgggctccgg
      121 gaacaaccga tacgccgttc gcacgatcac ggtgtcggga tcctcgatgt cgatttccat
      181 catcgggtcg ttgatctgtg cctccacggc gttttcacaa taaccgattt cgatcttcgc
      241 catgtagaag ccttcgatct cggtcggttc gatttcgatg cgccaggtga agcccgggta
      301 gatgatgcca aaatcacctt tgacctcgcg ctcgagcatg tccggctgga taacaccagc
      361 ctcgacctca gaaagcttgc tgtccaccag catcatcgcc tgggtataga gccgattggt
      421 tttggcgatt ttcaggccgg tgtcgatctg caaaccgacg acggccagac cgaacaccaa
      481 caggcccagc gcaacgacga cctccagcaa catcacgccg cgccgaccac accgagcccg
      541 gcgaatcgcg ccggcaacgc cgggcggcac cggcaactcg accggcacgg ccgggcaact
      601 ccggaacgct acgcgctgca tggcagactc tcctgattcg ccaccggtcg ttctcaattt
      661 cccaatcggc cccatcgccc caatcccccg ataccctttc gcccgacaaa ccgccggcga
      721 gaacccgccc tggccgtcat cgcgatccca cggccctcgt ccgccgaatg tccaggatgt
      781 tcgcctccgt aatcacttcc gggctgacga agtcgacatg catgatcggc tcggccttat
      841 actcatgcat gatctcgacc tcgtcgttca acatcgcccg ctgcatccac acctggccgg
      901 tacgaccgtc gacaatcaga tacaggattc gcgtgaagtg gtgcggctca agttccgtgt
      961 cgaacggcag atccgtcagg ataaaggtca cccattccgt cgtaccatcg ggaaacaggg
     1021 tgagcggcac gaactgagtc ttgtcctccg gcatgcccgg atcggcaatc ggccaacggg
     1081 tgttgatggc atagctgggt ttgccgggca gcaccgaaac gcatcgcgtg ccggcctgca
     1141 aaataggctg gttcttccag tcggcgtcga acccgccgta cgcttccggg ctgccctgcg
     1201 gatcgatctg ccgaaccacc tcgggttgaa gcgtctcgaa gggaacgtcc acttccgtct
     1261 ctgcattcgg atcgttggga tcaggggtac cggggaagga gatgcggtaa cgaatgccct
     1321 cctgcatcgc tctggcgtgg gccatctcga tcagagcccg cagtcgctcc gccgactcgg
     1381 tcaaagaccg gtgtcggcga tcggactcaa agctgggata gaccaaggcc atcagcaggc
     1441 ccatgagggc aatcacgagc aggacttcca gcaatgtaaa cgctcgaaga cgcatcgtga
     1501 ccttccattt ccgccgcggc tgacggccgg caacgctccg gcgaccccga cccgtcacaa
     1561 agccaacccg tgtgatcccc tcgcggcgaa gcacacgggt cacggctgga caagcgatct
     1621 gctacttgtc cttcgtccag ttgcagatgt cgtcgtcggt gccatcttca ccgtccggcc
     1681 ccaggctcca gatgtcgtag ctgccctcgt tggccttggc ttcgtcgttt ccaaggtact
     1741 ggaactcgtg accccaggca tccttgagtt tgtcgaggct gtcaatgtaa ggacccttcc
     1801 atttctccgc ctcagcatcg tcttcgggct tgtccctgag ttcggcgagt tccttcggaa
     1861 agcgtcccat gtgcagattg aacaactgca tcacgctggc cagactgtcc atattggcct
     1921 tcgtcgtctt gatgtacgcg tccctctgcg tgccgatcag gctgggcacc acgaaggccg
     1981 ccagcaatgc cagaatgccc accaccacca gaatctcgag cagcgtaaac ccgcgacgac
     2041 gattccgaac tctcttgacc tgtagcattt gtcaactcct tacatgagcc ggcccggcct
     2101 tcgatccgga ctacttctcc tgttttctca cttgtccaac gtcaccgggc acacttgcac
     2161 gcccggacaa tctcgtacct gtcaccccag gcccgccgaa cccatgcgaa ggatcggcac
     2221 caacagggcg atcgcaatga ccaataccat ggccgccata accatgagca gcagcggttc
     2281 aagcaaacgc acgccgaaat ccaccagccg gctggtgcgg gcctcgttcg aatcggcgat
     2341 ctgcaccagc acggcgtcga gattgttgct ctcctcggcc accgcaatca tgtccacaat
     2401 gtcggccgga aagagtccgc ttcgccccaa ccgtgcggcc agcgactccc cccgctgaac
     2461 gttctcagcc gcctggtcga tggcctcggc caggatgcga ttcccggccg agtccttgga
     2521 aatcttcagc gcctgcagaa tcgtgacacc gttgcccagc agcgttccca ggatgcggca
     2581 gaaccggcag atggccacca tggtcagtat tcgccccaga accgggacgc gcagcttcca
     2641 gcggtccagg agacgccggg cctcttctcg acgcgtcagc ccgactgcca gaaccacgag
     2701 tgcgacaaca acaccgagaa tcacccagcc atgattggcg acgaaatcgc ccaccgcgaa
     2761 caacacggtc gtcaaccatg gaacctgctg gcgctcgagc agcggcttga tcttcggcac
     2821 gacgaacccc atcaggaacg tcaccacgga gacgccggcc agcatcaaca cgcacgggta
     2881 gatcatcgcc ccgataacct tgttgcgaag ctcgtcctgc cgctcggtaa aaaccgcgat
     2941 gcgcgctaag acatcctcaa ggaaaccgcc ctgttcgccg gcgtgtacca tggagactgc
     3001 caagtgagta aacgcgttgg ggtgcttgct cattgcctcg gccagcgatt caccactcga
     3061 gacgtcggtg tgtacctctt tcaggatctc ggccagcagc gggttggccc cctgacgcga
     3121 caatacgtcg agcgcccgga gaaccggcac gcctgccctg aggaggtcgg atagctggct
     3181 atagaaagtg gtcaagactc gaagcctgac cttcttcctg cgaccggtta tggtagcacg
     3241 ggccgcggtt ccgccttcgg cgactttgat gggaaacaga gaccgctcgt ccagcatgcg
     3301 cattgccgcc tgctggcttt cggccgtcaa tacgcccgtg acggtctggc cacccgtagt
     3361 cttcgccttg tacgtgaacg tccccatacc gatccgctca aagcctgccc cccgaagaga
     3421 ggcctgcacc cccgccgcgc ccaacctgcc cgctcaaata gttatcgggc tacgagcaca
     3481 ttctcgcccc gctgaccttg gcaaatcagg cctttcttcc tgctcgcccg ccgaaatcca
     3541 ccggttggga cgacaaacta taaagacctg ctgaccaacg ttagcctaag agccacaaac
     3601 acttatgtca aacaaaccac cgctggcaaa caccggtacg acctccatgc acccaaacct
     3661 ggcggcagtc agcctcttat acgcacggca ggatcacgcg ccgcaaaccg cagccctatc
     3721 cccggaaggt aggccggtct gcgacgtgga tcataactat ttgtattaca atggcttata
     3781 atccgttatt ctgactgttt gaggtgcctg gcgaagaagt cccatgtttt ggccccgctg
     3841 aattcgtgag gaccgtcgaa aacatctgtt tccagggcct ctggcgcccc ggcgacccgg
     3901 tagcaccttt ggagcttgcg gagagcttcc tcggtcgccg cgatggggaa aatcgggtcc
     3961 tttcgtccgg cctcgatcaa caggggcttg ggcgcaatca ggcaggctag gtcatcgttg
     4021 ttggcctgca gcccgatacc cggaacgtag ttgtcgatgc aatggtgaat tgacaggatg
     4081 catgtctcgt aacgattgag gtaaccgctc acgcacgcgg ccctgatccg gtcgtccaag
     4141 gccgcggtga actgcgtcac cagccccccg ccactgatgc ccaccatgcc gatccgcttc
     4201 gggtcaacct cgggacggct ctgcaagaag tcgatcatgc gcatggcgtc ccacacacga
     4261 atgccggtca tgctcaaccc ccagtgcagg gcgttcttgc tcggctgctc gcagtggttc
     4321 cacatgttct gttgtttatg gaaatcaatg tcgcggcgcc tgccgaaacc catctggtcg
     4381 aagacgaggg taacgaaacc ggctctgacc gcctggatgg cgaaatcgtg ctgatagccc
     4441 tctttgtact ggcgcggctc gcccttctcg gtgagcgcga ccagttcgtt catgccgatg
     4501 ccgtggccgt ggctgcaaag gatggcagca cgcggctcct tgagaccgtc gggaaac
//
LOCUS       DAOTPW010000346         4548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15561_length_4548_cov_4.851992, whole genome shotgun sequence.
ACCESSION   DAOTPW010000346 DAOTPW010000000
VERSION     DAOTPW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15561_length_4548_cov_4.851992"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2818
                     /locus_tag="P5316_20495"
     CDS             <1..2818
                     /locus_tag="P5316_20495"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR87396.1"
                     /translation="NRLAIGVASLLWLIYRSGAQPRRLSYPCQQVAALNVGAFAAGLI
                     PALWLWRKPRCAHAVARRVVIRRQLLAAAILFVTALVGIEGYQYAQSLIPPSLPVVPA
                     RIDDPQHTIVGIAHRDPQGSWYTTAEIEDMVRLAIARAGGLDHLMTDRNNDGQISVIL
                     KPNLVQAEDPTDGVVTDPKVCAAIVKVAKEAGATHVGIAEGTAVGPAGQATWDAMYAA
                     GYDANRDYKFDYDTTVNLYDLNDSGGLHQTDPNKVTLVTIPNGVIRTQYYVPNVLLNC
                     DVMICVPTLKNHYNGTVTLALKNRVGCAPNDIYHSHRSWGEGFQGKLALVHYTDDGFP
                     CTVAPCPSSSDENEIVQRTIVDLNLVRPQDFAVVDGLIGVTTGPNDKSVGGRTGKPSP
                     YLHMIVAGADSLAVDAACSLVMDYHPDYIPHLAWADSRGVLGTKDRSMITVVGDQMWK
                     VRSDDFPGNWGIGAVKNTDHTAPWIGNTSVNEGEAVANHQMIAVSDVGDDVGVVQATA
                     VAAILGPNLLANGDFEAGSAGWTPWKTTWGGGEAYDFASTEPGHMGNACLKLGGASTA
                     TSFGVYQQVSVQPGRTYRIDAIWKGRKLADQNWFEILLIDGPFSLQQADDPAYVKPNF
                     MFAYDDSTYGLAGPVGTTFGWLWTHEQYAPPVNQVDWNNRLGRRTATGNTMTVVLKAG
                     STGGGVEAWFDEIRLTEVLSESPIAHLANPSDPAGLEIETDHLPQGSFPAELRISVYD
                     AALNVGSIYRNVTVSTVPETPFVCVDRIAFEQTVFVGSDATDETFHVFNCGKIGSTLD
                     YTIEWDSQTINWLTVSPDTGSAVQGSPPNPHGIFYDTTHLKPGTYTAGIAVVGDGVNT
                     VRLSVTVNVVTVSSDLDRDGDVDQSDFGRLQACLSGTTPCSAGCKDADLNNDGFVQDA
                     DCRFLLGCMSGPDVTPDPTCDDGP"
     gene            3006..>4548
                     /locus_tag="P5316_20500"
     CDS             3006..>4548
                     /locus_tag="P5316_20500"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR87397.1"
                     /translation="MWRNRVAIAVASLVWLLWRSGSQPRRLSYPCQQAAAANLGWLAV
                     LVVPGLARWWRKQSGRAEHAADLATGSVALAGILFALIGAGVAVFSDAGDDYVPGHPA
                     IVPWTPVDVSTPTPLNSRLLVPTGNEAVVAVNRNPSVTYGTQPYGPGTNTAYDLTWQT
                     VADLHLGPYNNPLADLITDVDGDGTIEVLIKPNTVRYFADDGGERNPVYSHPAIIRPL
                     VDMLAIAGAQEIRIGDGSNGTGGLFAKMDQQGFTQAYLNQLMALWPGITVSRVDFQNL
                     SHWSWVDLGADAGEAGASTYYDSGYNDTDLVKSADGSASSYFGAVDPHGRPGPGKYNC
                     MGWQAVTDYVFDADVIIDLAKLKVHYYGVSTAILKNWVGITMFSTFNMSENYWCRIAH
                     EPTNPTSYEKTFGNDILWREVADLHRGALYWRDGAVHSTPQRRYLCILDAINCGERYH
                     DPDTPWPYWLHTMLAGVDPVAIDAVGARLQRYDFRNISIINNAHAASIGSPWPIGTAD
                     PGQVRV"
BASE COUNT          889 a         1521 c         1340 g          798 t
ORIGIN      
        1 caaccgcctg gccatcggcg tggcctcgct gctatggctg atctatcgaa gcggagccca
       61 gccccgccgg ctgagctacc catgccagca ggtcgcggca ctcaacgtcg gagcctttgc
      121 cgccggcctc attcccgctt tgtggttgtg gcgcaaaccc cgatgcgccc acgccgtcgc
      181 ccgccgcgtc gtcatccgtc gccagttgct ggcggccgcc atcctgtttg tgaccgcgct
      241 ggtgggaatt gagggatatc aatacgcgca aagcctgatt cccccctcgt tgcccgtggt
      301 tcccgcccgc atcgatgatc cgcagcacac gatcgtcggt atcgcccacc gcgacccgca
      361 gggatcgtgg tacacgaccg ccgaaatcga agacatggtc cgcctggcca tcgctcgtgc
      421 cggcggactc gatcatctca tgaccgatcg gaacaatgac ggtcagatca gcgtcattct
      481 caagccgaac cttgtccagg cagaggatcc gaccgacggc gtggtcaccg accctaaggt
      541 ttgcgctgcg atcgtcaagg tggccaaaga ggcgggagcc acgcacgtgg gtatcgccga
      601 aggcacggcc gtcgggccgg ccggccaggc aacctgggac gccatgtatg ccgcggggta
      661 cgacgccaac cgtgactaca agttcgacta tgacaccacc gtcaatctct acgatctcaa
      721 cgacagtggc ggtctgcacc agaccgaccc caacaaggtc accctggtga ccatccccaa
      781 tggggtcatc cgcacacagt actacgttcc gaacgtgctg ctgaactgcg acgtcatgat
      841 ctgcgtgccc acactcaaga accactataa cggcacggtc acgctggctt tgaaaaaccg
      901 cgtggggtgc gccccaaacg atatctatca ttcgcaccgc agttggggcg aaggtttcca
      961 gggcaagctc gcgctggtcc actacacaga tgatggattc ccctgcacgg tggcgccctg
     1021 ccccagttcc tccgatgaaa acgagatcgt tcagcgcacg attgtcgacc tcaatctcgt
     1081 ccgcccgcag gattttgccg tcgtcgacgg cctcatcggc gtgaccaccg gcccgaacga
     1141 taagagcgtc ggcgggcgga ccggcaagcc cagcccctac ctgcacatga tcgttgccgg
     1201 ggcggattcg ctcgccgtcg atgccgcctg ttcattggtg atggactacc acccggacta
     1261 catcccccac ctggcctggg ccgacagccg gggagtcctc ggcacgaagg atcgctcaat
     1321 gatcacggtc gtcggcgacc agatgtggaa ggtccggtcc gacgacttcc ccggcaattg
     1381 gggcatcggc gcggtcaaga acaccgacca tacggcacca tggatcggca acacctccgt
     1441 caacgagggc gaagccgtcg ccaaccatca gatgatcgct gtttctgacg tcggtgatga
     1501 tgtcggggtc gtgcaggcga cggcggtggc tgccatcctc ggtccgaacc tgctggccaa
     1561 cggcgatttc gaagctggat cggcgggctg gaccccatgg aagaccacat ggggcggcgg
     1621 cgaggcctac gattttgcaa gtaccgagcc ggggcacatg ggcaatgcat gcctgaaact
     1681 cgggggggcc tccaccgcca ccagtttcgg cgtctatcaa caagtgagcg tccagcccgg
     1741 cagaacctac cgaatcgacg ccatctggaa aggccgcaag ctggccgacc agaactggtt
     1801 cgaaatactg ctgatcgacg ggcctttctc attacagcag gccgatgacc ctgcctacgt
     1861 caaacccaac ttcatgttcg catacgacga cagcacatat ggcctcgccg gcccggtggg
     1921 aacgactttc gggtggcttt ggacccatga gcagtatgcc ccgcctgtca accaagtgga
     1981 ttggaacaac cgtttgggcc gtcggaccgc gaccggcaac acgatgaccg tcgtgctcaa
     2041 ggccggcagc acgggcggcg gcgtcgaggc atggtttgat gagatccgcc tgaccgaggt
     2101 gctcagcgag agccccatcg cacatctggc caatccctcc gacccggcag gcctggaaat
     2161 cgagaccgat caccttccgc agggctcatt ccccgccgaa ctgcggatat cggtctacga
     2221 tgccgccctc aacgtgggca gcatctatcg aaacgtcacc gtcagcaccg tcccggaaac
     2281 accatttgtt tgtgtggatc gcatcgcctt tgagcaaacc gtctttgtag gcagtgatgc
     2341 aaccgacgaa accttccatg tattcaactg cggtaagatc ggcagcaccc tggactacac
     2401 catcgagtgg gacagccaga ccatcaactg gctcaccgtc agcccggata ccggcagcgc
     2461 ggtgcaaggc tcgccgccga acccgcacgg catcttctac gataccactc atctcaaacc
     2521 gggaacttac acggccggga tcgccgtcgt cggcgacggc gtcaacaccg tccgtctctc
     2581 cgtgaccgtg aacgtggtta cggtgtcctc cgatttggac cgcgacggcg atgtggacca
     2641 gtcggacttc ggccggctcc aggcctgcct gagcggcacc acgccctgct ccgcaggatg
     2701 caaagatgcg gatctgaaca acgacggctt cgtgcaagat gcagattgca gattcttgct
     2761 gggatgcatg tcgggcccgg acgtcacgcc cgatccgacc tgcgacgacg gaccataacg
     2821 ggcgatgatt cgccctgtaa aacgggtggc ggtggccaat gaaaaacaga ctcccacgca
     2881 acgctgaatc ccattctcga gcgtccgggc cccgcgcggg tcggggcggt cggtcgccgc
     2941 acgcacaccg ggaggcctca acgtcaggac ggctctcccg cctcgggcag caccttggag
     3001 ccgtggtgtg gcgcaaccgg gtggctattg cagtcgcctc gctggtctgg ctgttgtggc
     3061 gcagtggctc acaaccccga cgcctgagct atccttgtca gcaggcggca gcggccaacc
     3121 ttgggtggct cgcggtgctg gtcgttccgg gactcgcccg ctggtggcgc aaacaaagcg
     3181 gtcgggccga acacgcagcc gacttggcca ccggctcggt tgccctggcg ggtatcctgt
     3241 tcgccctgat cggtgccggc gtggcggtat tctcagacgc cggcgacgac tacgttcccg
     3301 gccacccggc catcgtcccc tggacaccgg tcgacgtgtc cactcccacg cccttgaact
     3361 ccaggttact ggtgccgacc ggaaacgaag cggtggttgc ggtcaatcgc aacccttcag
     3421 tcacctacgg cactcagcct tacgggcccg ggaccaacac ggcgtatgat ctgacttggc
     3481 aaaccgtcgc ggatctgcac ctcggcccct ataacaatcc gctcgccgac ctgataacgg
     3541 acgtggacgg cgacggcacg atcgaggtgc tgatcaagcc taacaccgtc agatacttcg
     3601 ccgacgacgg tggcgagcga aaccccgtct actcgcatcc ggccattatt cggccgctgg
     3661 tggacatgct ggcgatcgcg ggcgcgcaag aaatccgcat tggggatggc tcaaacggga
     3721 ccggcgggct gttcgccaaa atggaccagc agggcttcac ccaggcctac ctgaaccaac
     3781 tgatggccct ctggcccggc ataaccgtct cccgcgtcga cttccagaat ctcagccact
     3841 ggagttgggt cgatctgggt gcggatgccg gcgaagcggg agcgagcacc tattacgaca
     3901 gcggctacaa cgacaccgat ctggtcaaga gcgccgacgg ctcggcatcc tcttacttcg
     3961 gggcggtgga cccccacggc cggcctggcc ccggcaaata caactgcatg ggctggcagg
     4021 ccgtgacgga ttacgtcttc gacgccgacg tgatcatcga cctggccaag ctgaaggtgc
     4081 actactacgg cgtctcgacg gccattctca agaactgggt cggcatcacc atgttctcca
     4141 ccttcaacat gagcgagaac tactggtgcc gcatcgcgca tgagccgacc aacccgacat
     4201 cctatgagaa gacttttggt aatgacatac tatggcgaga agtggccgat ctgcaccgcg
     4261 gcgcgctgta ttggcgggac ggcgccgtcc actcgacgcc ccaacgaaga tacttgtgca
     4321 ttcttgacgc gatcaattgc ggcgagcgct accatgaccc cgacacaccc tggccctact
     4381 ggctccacac catgctcgcc ggcgtcgatc ccgtcgcgat cgacgccgtg ggcgccaggc
     4441 tccagagata tgacttccgc aatatctcga tcatcaacaa tgcccacgcc gcctcgatcg
     4501 gctccccctg gcccatcgga accgccgacc cgggtcaggt gcgcgttg
//
LOCUS       DAOTPW010000347         4529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15647_length_4529_cov_3.885561, whole genome shotgun sequence.
ACCESSION   DAOTPW010000347 DAOTPW010000000
VERSION     DAOTPW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15647_length_4529_cov_3.885561"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..361
                     /gene="rplW"
                     /locus_tag="P5316_20505"
     CDS             50..361
                     /gene="rplW"
                     /locus_tag="P5316_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRR87398.1"
                     /translation="MDIYHTIIRPLVTEKGTHQSQVSHERTRTRPARGGAYTFEVHPK
                     ANKAQIRQAIEKIYNVRVMSVRTSNRSGKRRRYRLTIGTTRGWKKAVVVLHPDYHIDL
                     F"
     gene            409..1275
                     /gene="rplB"
                     /locus_tag="P5316_20510"
     CDS             409..1275
                     /gene="rplB"
                     /locus_tag="P5316_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRR87399.1"
                     /translation="MGIKQYNPTSPGRRAGSVSDFSELTDKRRRPTKCLLEPKKKTGG
                     RNHHGKITSWWRGGGHKRMYRLIDFKRRNDSGRAVVQAIEYDPNRSCHIALIEYPDKT
                     RSYILAPAGLKAGDWVESGEQVEPKPGNCMPLKRIPVGMDVHNIEMMPGQGGKLVRSA
                     GSSARLVAREGDWATLLLPSGEMRQVRVECRATIGQLGNADHQNIQIGKAGRKRHMGR
                     RPHNRGTSMNPIAHPLGGGEGRSGGGRHPCSPWGKLAKGGKTRSPRKNSNKRILRRRR
                     SVRYGRQVIRSK"
     gene            1309..1641
                     /gene="rpsS"
                     /locus_tag="P5316_20515"
     CDS             1309..1641
                     /gene="rpsS"
                     /locus_tag="P5316_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRR87400.1"
                     /translation="MTRSLKKGPFVDEKLFRKVLRAKETGSHAPIRTWSRRCTIVPEF
                     VGVNFEIHNGKVFHKLFVTEEMVGHKLGEFSPTRNFRGHGGKARAAGAGPAAVPAGGP
                     AKASSGGK"
     gene            1702..2055
                     /gene="rplV"
                     /locus_tag="P5316_20520"
     CDS             1702..2055
                     /gene="rplV"
                     /locus_tag="P5316_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRR87401.1"
                     /translation="MAHGTVKERQYRAVHRYARVSPRKARLVADLIRGQKINTAQDIL
                     RFTPKRASGLIANVLKSAIANANEQEADVRRLFVAEARIDPGPYFRRWRPKDRGRAHQ
                     ILKKTSHIIVVVEER"
     gene            2123..2827
                     /gene="rpsC"
                     /locus_tag="P5316_20525"
     CDS             2123..2827
                     /gene="rpsC"
                     /locus_tag="P5316_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRR87402.1"
                     /translation="MGQKTNPIGFRTGITLDWKSRWYAPKAAYGEFLIEDCRIRKYID
                     DRLNERPPYAAVSKVEIERTRNEVRVVLHTARPGLVIGPKGAEVDKLREELEDLIDRK
                     VNLNVVEIKNPDLDAQLVAAAVAEQLKKRSSFRRVMKMRCDATMAAGAKGVRIMCSGR
                     LGGAELARTETQKMGSIPLQTLQANVDYGFAVAKTKTGAIGVKVWIYLGNFGDEAASV
                     ESEEGAKPRHRRRGRR"
     gene            2895..3314
                     /gene="rplP"
                     /locus_tag="P5316_20530"
     CDS             2895..3314
                     /gene="rplP"
                     /locus_tag="P5316_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRR87403.1"
                     /translation="MALMPKRMKYRKSQRGRVRGVAHRGNTVAFGEYGLQSLETGWLT
                     ARQIEAGRMCATHFLHRQGRVYIRVFPHKPVSGKPLETRMGKGKGEPEYYVAVVREGA
                     ILFELGGVTEDAARRALARVAHKMPFRCRFVARRHAV"
     gene            3339..3578
                     /gene="rpmC"
                     /locus_tag="P5316_20535"
     CDS             3339..3578
                     /gene="rpmC"
                     /locus_tag="P5316_20535"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRR87404.1"
                     /translation="MKVSEIRDMKTEELHAEMDRLRRHLFDLRSQAVTENLQDPSMLT
                     KAKRDIARVYTVLRERGETGIEQKQYHLETLARHK"
     gene            3649..3936
                     /gene="rpsQ"
                     /locus_tag="P5316_20540"
     CDS             3649..3936
                     /gene="rpsQ"
                     /locus_tag="P5316_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019663552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRR87405.1"
                     /translation="MAENTAQVKGRRARKTMIGVVTSANKTPKTIAVRVHYTARHPKY
                     GKFLNRSLTVKAHDAASTARLGDRVELMECRPFSRTKRWRLVRVIESAPRD"
     gene            3976..4380
                     /gene="rplN"
                     /locus_tag="P5316_20545"
     CDS             3976..4380
                     /gene="rplN"
                     /locus_tag="P5316_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRR87406.1"
                     /translation="MIQQQTMVDVADNTGAKQVMVIRVLGGSTARAGKPRRRVAGVGD
                     IVICTVKKALANSDFMKPGNHKARVVKAVVVRAKHATRRPDGSYVRFDRNAVVLIDDR
                     NEPRGTRIFGAVARELREKGFMKIISLADEVV"
     gene            4423..>4529
                     /locus_tag="P5316_20550"
     CDS             4423..>4529
                     /locus_tag="P5316_20550"
                     /inference="COORDINATES: protein motif:HMM:NF012680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HRR87407.1"
                     /translation="MACHIRKDDLVEVIAGDHVGERGKVLKVFPKKGLVL"
BASE COUNT          956 a         1187 c         1656 g          730 t
ORIGIN      
        1 caagtttgac cgggtacggg ggcagccctg gagtaaagga tactgagcga tggacatcta
       61 ccatacgatc attcggccgc tggtgacgga gaaagggacg caccagagcc aggtatcgca
      121 tgagcggacc cgaacgcggc cggcacgggg cggagcctac accttcgagg tccatccgaa
      181 ggccaacaag gctcagatac ggcaggcgat cgagaagatt tacaacgtga gggtgatgtc
      241 ggtccggaca tcgaaccgca gcggcaagcg tcggcggtac cggctgacga tcgggacaac
      301 ccgcggctgg aagaaggcgg tcgtcgtgtt gcacccggac tatcacattg acctgttctg
      361 acggcggagt cgggctagag cagagaacag agggaattcg agcgggccat gggcattaag
      421 caatacaatc cgacgtcacc gggtcgccgg gccgggtcgg tcagtgattt cagtgaactg
      481 accgacaagc gccgccggcc cacgaagtgt ctgctggagc cgaagaagaa gaccggaggt
      541 cggaatcacc acggcaagat aacctcctgg tggcgcggcg gcggtcacaa gcgcatgtac
      601 cgactgattg acttcaagcg ccgcaacgat tccggccgag ccgtcgtcca agccatcgag
      661 tacgacccca accgttcctg ccatatcgcc ctcatcgaat accctgacaa gacgagaagt
      721 tacatcctag ccccggccgg tctgaaagcc ggagattggg tggaaagcgg cgagcaggtg
      781 gagccgaagc cgggcaactg tatgccgctg aagcggattc cggtgggcat ggacgtgcac
      841 aacatcgaga tgatgccggg ccaaggcggc aagttggtgc gatcggcggg gtcgagtgct
      901 cggttggtgg cccgcgaggg tgattgggcg acgctgctct tgccgtcggg cgagatgcgt
      961 caggttcggg tggaatgtcg ggcgacgatc ggccagttgg gcaacgccga ccaccagaac
     1021 atccaaattg gcaaggcggg gcgtaagcgg cacatgggcc gccggccgca caaccgcggc
     1081 acctcgatga atccgatcgc tcacccgctg ggcggcgggg agggccgaag cggcggagga
     1141 cgccatccgt gcagcccttg gggtaagctc gccaagggtg gcaagacgcg ctcgccgagg
     1201 aagaactcga acaagcgaat cctgcgccgc cggcgaagcg tgcggtatgg acggcaggtg
     1261 atccggagca agtagacgtc ggagctcagg atcgggagcg aaccgaagat gacgcggagt
     1321 ctgaaaaaag ggccgtttgt tgacgagaag ctgtttcgca aggttctgcg cgcgaaggag
     1381 acgggcagcc atgccccgat tcgcacgtgg tcccggcgat gcacgatcgt tccagagttt
     1441 gtgggtgtca acttcgagat tcataacggc aaggtatttc acaagttgtt cgtgaccgag
     1501 gagatggtcg ggcacaagct gggcgaattc agtcccaccc ggaacttccg aggtcatgga
     1561 ggcaaggcga gggcggcggg agccgggccg gccgcagttc cggcaggcgg gcccgcgaag
     1621 gcttcgtcag gcggcaagta aggtggaagg ggccgtcggc ggtcaggccg gtgcccgcag
     1681 gttccacaga ggtagacgat cgtggcacac ggaacagtga aagaaaggca atatcgggca
     1741 gtgcatcgct atgcccgcgt cagtccgcgc aaggccagat tggtggcgga cctgatccgc
     1801 ggtcagaaga tcaacaccgc gcaggatatc cttcgcttca cgccgaagcg ggccagcggg
     1861 ttgattgcaa acgtgctgaa gtccgcgatt gccaatgcca atgagcagga ggccgacgtt
     1921 cggcggctgt tcgtggccga ggcccggatc gacccgggcc cgtactttcg ccggtggcgg
     1981 ccgaaggatc gtggccgggc gcatcaaata ctgaagaaga cgagtcacat tattgttgtg
     2041 gttgaggagc ggtgagcaga agcctttggt cgcgggttca caggcggacc cgttccgaga
     2101 ggccgagagc cgggagctga gtgtgggaca gaaaaccaac ccaatcgggt tccgaaccgg
     2161 tatcacgctg gattggaaga gccggtggta cgccccgaag gcggcctatg gcgagttcct
     2221 catcgaggac tgtcggatcc gcaagtacat tgatgaccgg ttgaacgagc ggccgccgta
     2281 cgccgcggta tccaaggtgg agatcgagcg gacccgcaac gaagttcggg tggtgctgca
     2341 cactgctcgc cccggtctgg tcatcggacc gaagggtgcg gaggtggaca agcttcggga
     2401 ggagctggag gacctgattg accggaaggt caacctgaat gtggttgaga tcaagaaccc
     2461 ggatctggat gcccagctcg tagctgcggc ggtggctgag cagctcaaga agcggagcag
     2521 tttccgacgg gtgatgaaga tgcggtgcga tgccacgatg gcggcggggg ccaagggtgt
     2581 tcggatcatg tgttccggcc gactgggtgg cgccgaactg gcccgcaccg agacgcagaa
     2641 gatgggatcg atcccgctgc agacgcttca ggcgaacgtg gactacggtt tcgcggtggc
     2701 caagaccaag acgggagcga tcggggtgaa ggtgtggatc tacctgggta atttcggcga
     2761 cgaggccgcg tcggtcgagt cggaggaagg agccaagccg cggcatcgac ggcggggccg
     2821 caggtagttg ggctggatgg ggcagcgggc ttggcggccc ggcagcccgg agtattgaac
     2881 ggagagcaat cgccatggcg ttgatgccga agcgaatgaa ataccgcaag tcgcagcgcg
     2941 ggagggtgcg aggtgttgcg catcgcggta acacggtggc cttcggcgaa tacgggctgc
     3001 aatcgttgga gaccggctgg ctgaccgctc ggcagatcga ggcgggccgt atgtgtgcga
     3061 cccatttcct gcaccgtcag ggaagggtgt acattcgggt gtttccgcac aagccggtgt
     3121 ccggcaagcc gttggagacc cgcatgggca agggcaaggg tgagccggaa tactacgtgg
     3181 ccgtcgtgcg cgagggtgcg attctgtttg aactgggcgg tgtgaccgag gacgcggccc
     3241 ggcgggcatt ggcccgggtg gcccataaga tgcccttccg ttgccggttc gtggctcgac
     3301 ggcatgcggt gtgacgtgcg gcgacgagga gttgaggtat gaaagtatcc gaaatccggg
     3361 acatgaagac cgaggagctc catgccgaga tggaccggct tcgccggcat ctgttcgatc
     3421 ttcgcagcca ggcggtgacc gagaatctgc aggatccctc gatgttgacc aaggccaagc
     3481 gggacatcgc ccgggtttac accgtgcttc gcgagcgtgg tgaaacgggc atcgaacaga
     3541 aacagtatca cctggagacg ctggcaaggc acaagtagtc ggagcgtccc gcgtggaagc
     3601 gggagtcgga cggccggacg caggccgaaa ggaatcaggt agcaggcgat ggcagagaac
     3661 acggcacagg tcaaagggcg acgggcccgc aagaccatga tcggtgtggt gacgtcggcg
     3721 aacaagacgc ccaagacgat cgcggtaaga gtgcactata cggcccggca cccgaagtac
     3781 ggcaagttcc tgaatcgatc gctgacggtg aaggctcacg atgcggcctc gacggcgagg
     3841 ttgggtgacc gcgtggaact gatggaatgc cggccgtttt ccaggaccaa gcggtggcgg
     3901 ctggttcgcg tgatcgagtc ggcgccccgg gactgacgta agccgccggc ggctcggtat
     3961 ggacgagata gagcgatgat tcagcaacag accatggtgg atgtggcgga caacaccggc
     4021 gccaagcagg tgatggtcat tcgcgttctc ggaggcagta ccgcgcgggc cggcaagccg
     4081 agacgccgag tggccggggt gggagacatt gtcatctgca cggtgaagaa ggcgttggcc
     4141 aacagtgatt tcatgaagcc gggcaatcac aaggcgcgcg tggtgaaggc ggtggttgtg
     4201 cgggccaagc atgccacccg gcgtccggac ggcagttacg tgcgttttga tcggaacgcc
     4261 gtggtgttga tcgacgatcg gaatgagccg cgagggacgc ggattttcgg ggcggtggcc
     4321 cgcgagcttc gggagaaggg gttcatgaag attatctcgc tggcggatga ggtggtttga
     4381 acggcttgag cgcaaggcgc aagtgaattc ggaagcggaa caatggcttg ccacatcagg
     4441 aaagacgatc tggtcgaggt gattgccggt gaccacgtgg gcgaacgcgg caaggtgctg
     4501 aaggttttcc cgaagaaggg cctggtgct
//
LOCUS       DAOTPW010000348         4507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15743_length_4507_cov_3.903639, whole genome shotgun sequence.
ACCESSION   DAOTPW010000348 DAOTPW010000000
VERSION     DAOTPW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15743_length_4507_cov_3.903639"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..243
                     /locus_tag="P5316_20555"
                     /pseudo
     CDS             1..243
                     /locus_tag="P5316_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272705.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
     gene            complement(298..816)
                     /gene="rplD"
                     /locus_tag="P5316_20560"
                     /pseudo
     CDS             complement(298..816)
                     /gene="rplD"
                     /locus_tag="P5316_20560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
     gene            complement(882..1535)
                     /gene="rplC"
                     /locus_tag="P5316_20565"
     CDS             complement(882..1535)
                     /gene="rplC"
                     /locus_tag="P5316_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRR87408.1"
                     /translation="MVAALLGRKVGMTRVYTENGEVVPVTVIQAGPCSVLATKSAAGP
                     DKYDAVQLGFEDVKPHRSTLPMIGHAAKAGTGPKRLIREVRLKEAPQASPGDVWTVEL
                     FESAQVRYVDVVGTTKGRGYAGVMRRHHFGGQPNSHGTERKHRSPGGIGGMAQRGRGR
                     CIKKGKRMAGHMGNARRTSRNQELIRVDKENHLLLVKGSVPGPNGGYVLVRQAKAKK"
     gene            complement(1664..1972)
                     /gene="rpsJ"
                     /locus_tag="P5316_20570"
     CDS             complement(1664..1972)
                     /gene="rpsJ"
                     /locus_tag="P5316_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRR87409.1"
                     /translation="MQGERIRIRMEAYDPRALDASAREIVDHAKRTNARVCGPIPLPT
                     RIERYTVLRSPHIDKKSREQFEMRTHKRLIDIYEPTARTVEALNRLVVPAGVFVKIKA
                     "
     gene            complement(2335..4446)
                     /gene="fusA"
                     /locus_tag="P5316_20575"
     CDS             complement(2335..4446)
                     /gene="fusA"
                     /locus_tag="P5316_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRR87410.1"
                     /translation="MSLDLRKVRNIGIAAHIDAGKTTTTERFLYYTGKTHKMGEVDDG
                     TTVTDFDQEEQQRGITIYSAAVSCPWRDHTINLIDTPGHVDFTAEVERSLRVLDGAVV
                     VFDAKEGVEAQSETVWRQASKYRVPRICFINKMDKVGADFAASFKSIRERLGANPIPV
                     QIPIGAESHFEGIVDLLEMKAIYYEREQLGAKFRIVDIPAKLQDAAREARHELEERLA
                     EVSEPIMEKFVHDEPIIADDLKPAIRAATVADLAHPVFCGSALAYIGIQRLLDGVIDY
                     LPSPLDCPPIVGQVPDLADKSHKGRSKEKAKGGTVECRCDPTGPLVAYVFKVVAEKPV
                     DVFFVRVYSGTLKTNSRVLNPARGVKENVSRILRVFAKRREPIDSAVAGDIVAVTGLK
                     ESLTGDTLCDPKHPVLLEKIEFPETVVSMSIEPQSSADRDRMLEALAMLSRENPTFEY
                     RANEETGQTLISGMGELHLEVLVNRLRRDMKVDVRVGTPRVSYRDTITAVAVCEEEFK
                     RQTGGKGLYARVKLQLEPFTPKEGEEHVVFESRVPEGAIRPEFLAAVANAVKDAARTG
                     LRGYPIINLKIILLEAAEHETDSTEGTFEAAARMAFDRAAESAQLALMEPIMRVEVIT
                     PESYFGAVSGDLTRRRGVVNDATVRGGQRVIEAMVPLREMFGYATDLRSLTQGRGSWT
                     MEPSHYAIVPPSIGDAIIAAS"
BASE COUNT          798 a         1589 c         1246 g          874 t
ORIGIN      
        1 gccgcctacg gcgtggcact ggcgatgatg cggcaggccg acgacgcggc gctgctggcc
       61 gccgtcgaga cgctggccgc gacccggccg acggcggtaa acctgcgctg ggcgctggcg
      121 cgcgttcaga cctgtctgac gccgctgccc gcttcggcgc gtgccgaagc ggcgtggcgc
      181 gaggcggccc ggattgccga tgaggacgtc gaccagtgtc gccggatcag ccagcacggc
      241 atccagcgcc gccttcgaca agagaacctt ccgacggcgc agaatctcat acgcgttgag
      301 atcccctact tgtcgaatct ccgtcttggg gatgttgcgc ccggacttgt ataccgtcac
      361 attaggctga tcgatcgcca gcacgcaacc ctgatccgcc ccaacggcct tgaacaacgc
      421 gtagagttcc ttcgtcttgg gcttgtccat cgtcaggggg tcaatcaccg caaccgcacc
      481 ggccttcatc ttggccagaa gagcgctgtt ccgggccgct cgccgcatct tgcgcggcat
      541 ctcgcggcgg taattacgct ccagtttggc aaaggccacc ccgccgccgc gtcggatcgg
      601 cgtccggacc gtacccatgc gggcgttccc cgtgcccttc tgccggtaca acttgcgcgt
      661 cgatccctcc accatggacc ggcatttcgt gcgcgcggag ccctgccatt ggttcatctg
      721 aaacgccgag atggcctgct tgagcaggtc gtagcggacg cggccgccca ggatggcctc
      781 gtcaacctgg accgctccgg tccgttcgcc actcatgttg tatacaggta cctcgatcat
      841 cacctgcact ccgctgccgc cccagggcgg ctcacgccac cctacttctt ggccttggcc
      901 tgccgcacca gcacgtaacc gccgttcggc ccaggaacgc tccccttcac cagaagaaga
      961 tggttctcct tgtccacacg gatcagttcc tgattgcggc tggtccttcg cgcattgccc
     1021 atgtgaccgg ccatgcgctt gcccttctta atgcaacggc ctcgaccccg ctgagccatg
     1081 cccccgatac cccccggaga acgatgcttc cgctccgtcc cgtgtgagtt cggctgacca
     1141 ccgaagtggt gacgccgcat gacgcccgcg taaccccgcc ccttggtcgt tccaacaacg
     1201 tccacgtacc ggacctgggc gctctcaaac agctcaaccg tccatacatc cccaggactg
     1261 gcctgcgggg cctccttcag gcgaacctcg cgaatcagcc gcttcggacc ggttcccgcc
     1321 ttggcggcgt gcccgatcat cggaagcgtc gaccgatgag gcttcacgtc ctcgaagccc
     1381 agttgcacgg catcgtactt gtccggtccc gcggccgact tggtcgccag aaccgagcac
     1441 ggccccgcct ggatcacggt caccggcacg acctcgccgt tctcggtata cacccgagtc
     1501 attccgactt ttctgcctag cagtgccgcc accatgtgaa aagacttccc ttcaaagccc
     1561 tgcgggctca tcctcttggg tcatgccgcg cgatcaagct cacacggcca agctgctgaa
     1621 aaacgatgac tgtaggagcg aaaaagccgc cgcccagtcg gtatcaggcc ttgatcttga
     1681 cgaacacccc cgctggaaca accagccgat tcaaggcttc taccgtccgc gcggtcggct
     1741 cgtagatgtc gatcaaacgc ttgtgcgttc gcatctcaaa ctgctcgcgg gacttcttgt
     1801 caatgtgcgg cgagcgcagg acggtgtaac gctcgatccg ggtcggcagc ggaatcggac
     1861 cacacacccg cgcgttcgtc cgcttggcat gatccacgat ctcgcgggcc gatgcgtcca
     1921 atgcccgcgg gtcgtatgcc tccatcctga tccggatccg ttcgccctgc atgaccttcc
     1981 tctaaatgct tcttgcggcc ctttgcggcc ttgtctcact gctcccgccc gcacccacaa
     2041 ccccgaaaaa gacccggcaa cccccgtcgc tcccgcctcc gcgggcaatc acacgggctg
     2101 ccatcggcct cgtcatcggt tccggtccgc caaaccctcc gcgctaaacc tccacggcga
     2161 cagggtttgc atgggttaga cgcagacccg taagtttagc catcctgagg aatctgtcaa
     2221 ccccagcccg ccgtgatttt ttccgctttt ccgacgtccg tcagcccgcc gctttccgag
     2281 atagttatcg gacgctagtg cgccgcccat caaaggattc aaactgacat aacttcaact
     2341 cgcagcaata attgcgtctc caatagaggg tggcacaatc gcatagtggg acggctccat
     2401 ggtccagctc ccacgacctt gggtaagact gcggagatcg gtggcatagc cgaacatctc
     2461 ccgaagcgga accatcgcct cgatcaccct ctgcccccca cgtaccgtcg cgtcgttgac
     2521 caccccccgg cgacgggtaa ggtcgccact gaccgcccca aaatagctct ccggcgtgat
     2581 gacctcgacc ctcatgatcg gctccatcag ggcaagctgg gccgactcgg ccgcccgatc
     2641 gaaggccatt cgggctgcgg cctcaaaggt gccctccgtg ctgtccgtct cgtgctcggc
     2701 ggcctccagc aggatgatct tgagattgat gatcgggtag ccgcgaagac cggttcttgc
     2761 cgcatccttc accgcattgg ccacggccgc cagaaactcc ggccgtatgg ccccctccgg
     2821 caccctgctc tcgaaaacca cgtgctcttc gccttccttc ggagtgaacg gctcgagctg
     2881 caacttgacc ctggcataga ggcccttgcc gcccgtctgc cgcttgaatt cctcctcgca
     2941 gacggccacg gccgtgatcg tgtcccgata cgagaccctg ggcgtcccga cgcggacgtc
     3001 aactttcatg tcacgccgca accgattgac aagcacctcg agatgaagct cgcccatgcc
     3061 gctgatcagc gtctgcccgg tttcctcgtt cgcccggtat tcgaacgtcg ggttctcgcg
     3121 ggacaacatc gcaagcgcct ccagcatgcg gtcacgatcc gccgacgact gcggctcgat
     3181 cgacatgctg accaccgtct cgggaaactc gatcttctcg agcagcaccg gatgcttcgg
     3241 atcgcacaac gtgtcgcccg taagcgattc cttcaaaccc gtcacggcca cgatatcacc
     3301 cgccacggca ctgtcgatcg gctcgcgccg cttggcgaag acgcggagga tccggctgac
     3361 gttttccttg acgccgcggg ccgggttgag cactcgcgag ttcgtcttca atgtccccga
     3421 gtaaacgcga acaaaaaaaa cgtccaccgg cttctcggcc acgaccttga acacgtatgc
     3481 aacaagcggg ccggtcggat cgcagcgaca ttccaccgtg ccgcccttgg ctttttcctt
     3541 cgacctgccc ttgtgcgact tgtcggcgag atccggcacc tggccgacga tcggcggaca
     3601 atcaagcggg ctcggcaggt agtcgatcac gccgtcaagc agccgctgga taccgatata
     3661 cgccagcgcc gacccgcaaa acaccggatg ggccagatcg gccaccgtgg cggcgcgaat
     3721 ggccggcttc aggtcatcgg cgatgatcgg ctcatcgtgg acgaacttct ccataatcgg
     3781 ctcggagacc tcggccagcc gctcttcaag ctcatggcgg gcctcgcggg cagcgtcctg
     3841 aagcttcgcc gggatgtcta cgattctgaa cttggctccc agttgctccc gctcgtaata
     3901 gatggctttc atctcaagca ggtcgacgat gccctcgaaa tgggactcgg cgccaatggg
     3961 aatctgcacc ggaatgggat tggctcccag acgctcgcgg atcgacttga acgaggccgc
     4021 aaagtccgcc ccgaccttgt ccattttgtt aataaagcag atccgcggta cacgatactt
     4081 gctggcctgc cgccagacgg tttccgactg ggcttccaca ccttccttgg catcgaacac
     4141 caccaccgct ccgtccagca cccgcagcga ccgctccacc tcggcggtga agtccacgtg
     4201 gccgggggtg tcgatcaggt tgatggtgtg gtcgcgccag gggcaactga cggccgccga
     4261 gtagatggtg atcccccgct gctgttcttc ttggtcaaag tcagtgacgg tggtaccgtc
     4321 gtcgacctcc cccatcttgt gcgtcttgcc ggtgtaatag aggaatcgct cggtcgtcgt
     4381 cgtcttgccg gcgtcaatgt gggcggcaat cccgatattg cgtactttcc gcagatccaa
     4441 agacacaatc cagactccca gatcgccgcc acccgggtcc ccgatcgctc caggttgccg
     4501 ccttcac
//
LOCUS       DAOTPW010000349         4488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15825_length_4488_cov_4.932326, whole genome shotgun sequence.
ACCESSION   DAOTPW010000349 DAOTPW010000000
VERSION     DAOTPW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15825_length_4488_cov_4.932326"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="P5316_20580"
     CDS             complement(<1..1376)
                     /locus_tag="P5316_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87411.1"
                     /translation="MMAVVVSLVAPGPMQADWELQTVDSATTAVALALDGDGIAHLCY
                     FTPPIDHLSGTTGHLLKYANNAGGPGIWTTETIETLTKTGDGCDIAVAADGTVRVAYI
                     VGDDLKYARRDGGSWMVTTAVSGGVKWRCALALDSSNTPHIAFSRFSDYDDPYDRVMY
                     QGLPCYVTWTAGAWQETALQWAAGEPEYLLDTGEWQDIALSSDETVYGTWKSAWTLRF
                     MVPLSHDPSHFAGYQTGLPGTANRFRIDAFDDFYAAGIGSDTGAVVLGRNTSDPSDET
                     SWYFFWERYELDNTGTHYGDDSEQHLGLAADSEAGAHLVFYDAIDNDLKYIWQSASWA
                     YGESSPVQRIHPCFTELVDSDGDVGRSADISVDGSDDPHIVYLDTSNHAVKYGKRMAF
                     SGPQLRVLPSRWDYGHAEIWPTENRKEFIIMNPGSEDLVITSIGVDDVTSYPAGGAPP
                     FRIWAVKLT"
     gene            1727..3208
                     /gene="gatB"
                     /locus_tag="P5316_20585"
     CDS             1727..3208
                     /gene="gatB"
                     /locus_tag="P5316_20585"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRR87412.1"
                     /translation="MDRSQLKIRPIIGLEIHVQLATRSKMFCSCPVEFGAEPNSNVCP
                     VCLGMPGVLPVMNRAALEHAMRTAIALRCQIARFTKWDRKSYYYPDLPKNYQISQYDL
                     PLSFDGAFDVPLPNGEIRRVGIIRAHLEEDAGKNIHDNPSVTLVDLNRTGTPLLEIVT
                     RPDLASADEAYAFCIELQRLVTYLGVSEGSMQKGQMRFEPNINVAIEYDGREYRTPIC
                     EVKNLNSFRAVRDAIAYEIERHINDWMADPGYVIGKSPKENRGWDDVRGVTEFQRGKE
                     EAHDYRYFPDPDLVPVVVDDEWLERLQASVGELPLERQARMESEYGLTSADAATILND
                     RATADLFEEAAGCGHPPTLGKQFISFWSARANERKCTIAQLGIDAARLGELSRITAEG
                     LVSATAAATIAGKMLDCPDTPTAIAQREGLVQVRDTGQMQTWVDQAFAANEKAVRDAL
                     ANPKKKQQARGFLTGQVMKISGGKADPKMVGELIDQKLEGMSS"
     gene            3349..3882
                     /locus_tag="P5316_20590"
     CDS             3349..3882
                     /locus_tag="P5316_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87413.1"
                     /translation="MESILELLQFRSAAPFAYLGFCCVSVAAVAAGTVLIVQGLRKRP
                     RSRWRIAIGALLSCGIAMQFAANVLFESMIEFNPSAVNRQDLHGLWSDRGTTLQVNAD
                     GTLDLKAKQPLATRIGATQATGTWSIDDWNLRLRTASGTSLTLLRVIQFGKTYRLIIE
                     DYGDPDCWDGRLGFRRR"
     gene            3949..4173
                     /locus_tag="P5316_20595"
     CDS             3949..4173
                     /locus_tag="P5316_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87414.1"
                     /translation="MAGIKAHFDGRALVPDEPAQFRPGEQVVIERLGEAPSKAAPGVN
                     VSFGRKLNIDLDARSVCVVAEEPERSFESL"
     gene            4177..>4488
                     /locus_tag="P5316_20600"
     CDS             4177..>4488
                     /locus_tag="P5316_20600"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRR87415.1"
                     /translation="MPKRLGAHPRTLKSRKRDAARPLSPKSGRTSVSGGTPGRGKSAP
                     AVLKEIIRHIVRVAHPERIILFGSAGRGEMGPNSDLDLLVIKRGKFDRGRLIERIYLS
                     LH"
BASE COUNT          889 a         1376 c         1383 g          840 t
ORIGIN      
        1 gtcagtttga ccgcccaaat ccggaacggc ggagcaccgc ctgcggggta cgaagtgaca
       61 tcgtcaacgc cgatggaggt gatcaccagg tcctccgagc cggggttcat gatgatgaac
      121 tccttgcggt tctcggtcgg ccagatctcc gcgtggccgt agtcccaccg cgagggcaaa
      181 acgcgcagtt gcggccctga gaacgccatg cgcttgccgt acttgaccgc gtggttgctg
      241 gtgtcgaggt agacaatgtg cgggtcgtcc gatccgtcga cgctgatgtc ggcactgcga
      301 cccacgtcgc cgtcgctgtc gacgagttcc gtgaagcagg ggtggatgcg ctggacgggg
      361 cttgactccc cataggccca ggacgccgat tgccatatgt acttcaagtc gttgtctatg
      421 gcgtcgtaga acaccagatg cgcacccgcc tcggagtcgg cggccaagcc cagatgctgc
      481 tcgctgtcat caccatagtg ggttccggtg ttgtccagct cgtacctttc ccagaagaag
      541 taccaggacg tttcgtcgct tgggtcggag gtgttccgcc ccaagacgac ggctccggtg
      601 tcgctgccga tcccggccgc gtagaagtca tcgaacgcgt cgatccggaa ccggttcgcg
      661 gtgccgggca ggcccgtctg ataacccgca aagtggctgg ggtcatgaga aagcggaacc
      721 atgaatctca gcgtccaggc cgatttccat gtgccgtaga cggtttcgtc ggagctcagc
      781 gcgatatcct gccactcgcc ggtgtccagc aggtattccg gttcgcccgc cgcccattga
      841 agcgccgtct cttgccaagc gccggccgtc caagtcacat aacaaggcag gccttggtac
      901 atcacacggt cataagggtc gtcatagtcg ctgaatcggc tgaaggcgat atgaggggtg
      961 ttgctggagt ccaacgcgag cgcgcatcgc cacttgacgc cgcctgaaac cgctgtcgtc
     1021 accatccacg agccgccgtc cctccgtgcg tacttgaggt cgtcgccgac gatgtacgca
     1081 acacggacgg tcccatcggc cgccaccgcg atgtcgcagc cgtcgccggt tttcgtgagc
     1141 gtctcgattg tctcggtggt ccatatgccg ggcccgccgg cattgttggc atacttcaga
     1201 agatgtcccg ttgtgcccga caggtgatca atgggcggcg tgaagtagca gagatgcgct
     1261 atcccgtccc catcgagagc gagggccacg gccgtagtcg cgctgtctac cgtttggagc
     1321 tcccagtccg cttgcatcgg tccaggcgcg accaggctca cgacgaccgc catcatgacg
     1381 gcgctcagga acgttctgcc gcagcgggag tgcctgaccg gccatcttgt gcctgctgtt
     1441 ctcagttgct tctgtcccga catgctcgtg cacctctcgt atggacaacg gcaggcgccg
     1501 cggcgcaggg ttgaatcgcg agacacccat tcgaccgtct ccagcgtccg gagccgcctg
     1561 tctgacagta tacccacaac gcggcgcgca aagatagagc gacatgcgga tgctggcgac
     1621 gcagcgatca aagcgggcac caaccactgt gatgcctgct ggcggcatcg gctcagatgg
     1681 ggtacaacat attgcgtgtc tgagcatgaa cgagcgagaa tgagccatgg accgcagcca
     1741 actgaagatt cgcccgatca tcggcctgga gatccacgtt caactggcca cgcggagcaa
     1801 gatgttctgt tcctgcccgg tagagttcgg ggccgagccg aacagcaacg tttgcccggt
     1861 atgtctgggg atgcccggag tgctgccggt gatgaaccgg gccgcccttg agcacgccat
     1921 gcgcacggcc atcgccctgc gctgccagat cgcccggttc accaagtggg accgcaagag
     1981 ctattactat cccgacctgc ccaagaacta ccagatcagc cagtacgacc tgccgctgag
     2041 tttcgacggg gcgtttgacg tgcccttgcc taacggcgag atccgtcgcg taggcatcat
     2101 ccgggcccat ctcgaagagg atgccggcaa gaacattcac gacaacccct cggtcacgct
     2161 tgtggatctt aaccgcaccg gcacgccgtt gctggagatc gtcacccgac cggatctggc
     2221 ctcggccgac gaagcatacg ccttctgtat cgagcttcag cgtctagtga cctatcttgg
     2281 cgtcagcgaa ggcagcatgc agaaggggca gatgcgattc gagccgaaca tcaacgtggc
     2341 catcgaatac gacggccgcg agtaccgtac accgatctgc gaggtgaaaa acctcaacag
     2401 ctttcgggcc gtccgcgacg ccatcgctta cgagatcgag cggcacatca acgactggat
     2461 ggccgacccg ggttatgtca tcggcaagag ccccaaggag aaccgcgggt gggacgatgt
     2521 ccgaggggtc actgagttcc agcggggcaa ggaagaggcc cacgactacc gctatttccc
     2581 cgaccccgac ctggtgccgg tggtggtgga cgacgaatgg ctcgaacgat tgcaggccag
     2641 cgtcggcgag cttccgcttg agcgtcaggc tcggatggaa agcgaatacg gcctgacctc
     2701 cgccgacgcc gcgacaatcc tgaacgaccg ggccacggct gatctgttcg aggaggccgc
     2761 cggttgtgga catccgccca cacttggcaa gcagttcatc agcttctggt cggccagggc
     2821 caacgagcgc aagtgcacca tcgcccagct cggtattgac gccgcccggc tcggcgagct
     2881 gtcgcgcatt accgccgaag gtcttgtcag cgccaccgcc gcggcgacca tcgccggcaa
     2941 gatgctcgat tgccccgata ccccgacggc cattgcccag cgagaaggcc tcgtgcaagt
     3001 ccgcgacacc ggccagatgc aaacctgggt cgaccaagca ttcgccgcta acgagaaggc
     3061 cgtccgggat gccctggcca accccaagaa gaagcaacag gcccgcggct tcctcaccgg
     3121 ccaagtgatg aagatctccg gcggaaaggc cgatccgaag atggtcgggg agttgattga
     3181 tcagaagctt gaggggatga gttcttagga agcttttagc tcttagctat tagctcgggt
     3241 gtcgggtggc atggccacgg ctctgcgtgg ccatgagcgg ccgatttcaa acgcagaagt
     3301 agagattctg gacggccacg taaggaatcg tccgagagga cgtcgctcat ggagagcatc
     3361 ctggaactgc tgcagtttcg cagtgccgca ccatttgcct acctcggatt ctgctgtgtg
     3421 tccgtagcag cggtcgctgc tgggaccgtc ttgatagtcc agggcctgcg aaaacgccct
     3481 cggtctcgtt ggaggattgc catcggagcc cttctatcct gcggcatagc catgcagttc
     3541 gccgcgaatg tcctttttga atcgatgatc gaattcaacc cttcggctgt caaccggcag
     3601 gatctccatg gtctctggtc tgacaggggc acaaccctac aggtcaatgc cgacggcacg
     3661 ttggacctca aggcgaagca gccgctggca acgaggatcg gggcaactca ggcaaccggg
     3721 acatggagca tcgacgactg gaacctccgg ctcagaacag cctccgggac aagcctgacc
     3781 ctgctgaggg ttatccaatt cggcaagacc tacaggctca tcatcgagga ttacggcgat
     3841 cccgactgct gggatggccg acttggcttc cgacggcggt aaccgctcgt tgccccggat
     3901 ggtatctggc tcgtccgtca agcaagtata ctatgtaata aggtgcttat ggcggggatc
     3961 aaagctcact tcgacgggcg tgcccttgtg ccggacgagc cagcgcagtt tcgtcctggc
     4021 gagcaagtcg tgatcgagcg attgggcgag gccccgagca aggcggcgcc aggagtgaat
     4081 gtctctttcg ggcgtaaact caacatcgac ctggatgccc gctcggtctg tgtggtcgcg
     4141 gaagagccgg aacgaagttt cgaaagtctc tgagccatgc caaaacgatt gggtgcgcat
     4201 cccagaactc tgaaatcacg caagcgagac gcagctcgac cgctctcgcc gaagtccggt
     4261 cgcaccagcg tctcaggagg cacgccaggt cggggcaagt ccgctcctgc ggttctcaag
     4321 gagatcatcc gccacatcgt tcgagtggcc caccccgaga ggattatcct atttggctcc
     4381 gccgggcgtg gcgaaatggg acccaacagc gatcttgatc tgctggtgat caagcgaggt
     4441 aagtttgacc ggggccggct gatagaaagg atctacttga gcctgcac
//
LOCUS       DAOTPW010000350         4471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15889_length_4471_cov_5.520833, whole genome shotgun sequence.
ACCESSION   DAOTPW010000350 DAOTPW010000000
VERSION     DAOTPW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15889_length_4471_cov_5.520833"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="P5316_20605"
     CDS             <1..1065
                     /locus_tag="P5316_20605"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR87416.1"
                     /translation="DDGSTDDTPRIARQAGFALVRHPENRGVATARNTAIRTARGKLV
                     ASLDADQIAPRDWLARMLKNFEGRRRIVGCCGRVVEKHTDTIADRWRAVHMKLSFGMR
                     RSYDPRWLHGGTMLVRDAVLDVGLYNERCLRAYEDVELVARLKAAGHVLLYDPTIIAT
                     HLKRSRPGDVVRDFWSYWAAKNEIEGAYKSLAAACRLMIARQMGIAAYRIGQDLKHKR
                     DELLPLDAIIPLAFCVRDLDEMVRLGRLRREQALDIQTALTRCYQGVWEELFPRKCSG
                     DDCIAAGMARLAFTGGRLPDGRPNRLAAAAKNYVSAFRRAFAHLLADLPVPVRERILR
                     RASDVLLEAEPSMRAGWSGE"
     gene            1415..2077
                     /locus_tag="P5316_20610"
     CDS             1415..2077
                     /locus_tag="P5316_20610"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR87417.1"
                     /translation="MREEHQRDVAYVQLRHHLILQQIPPGSRLAEVEWAARLGVNRTA
                     LREAFARLASEGLIRRGEKTGYIVPVLTKKDEHDSLLVRVALEACAIDIICASGLNTP
                     SHLERLSAACDLLAQLVDEEYHLSTVEADWRFHQALIEAAGNEPLVIAYRHAPVLMLQ
                     ATIRRGPDWVQREERSIAEHRQVLAAMLSGDATEAKRVLRQHLVDSWEAERTSRKTME
                     SA"
     gene            2657..3628
                     /locus_tag="P5316_20615"
     CDS             2657..3628
                     /locus_tag="P5316_20615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR87418.1"
                     /translation="MKNLSNILVAFVGFELLLSPAVGGFLLENGGMEDPFAGSYPNVV
                     AVPWTYYSYGGLSGAQQSKETTVVHGGLSSQRLAGNATNNAAYFGIRQTIDANVGDAF
                     MFGGWVWPDSAGTYNETSIRVAWDGGTAALDATVLMNWLPLGSQKQQWHQMVTTSGST
                     LAGGNATATTITVFLHSDRNTSNGSLLTYWDDVVGYHTFVPPAPILGNQTPSSFEVNV
                     DPGGNSGNLFAQYAITVGGGGYTLGSDWLQADGSVGGSPVWRTDAQWGTTTVTGVAGD
                     TIYSVQVKARYDSVRAQETYLGPVAMIPEPVTVALLGLGIAAVIRRR"
     gene            3883..>4471
                     /locus_tag="P5316_20620"
     CDS             3883..>4471
                     /locus_tag="P5316_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87419.1"
                     /translation="MMNRQVVLILAACIGLISAQAWADTWCNSSYPPDPPACHSAAIH
                     CEDVDRICNGAPACPPPPPEPCPGQDWSWMLIRQAFPRTSRINDTGSECGVEFKGEED
                     TKLFTSYPLGTRYPYGDLGQATIDLSGHIRYTFGSDEYTAVVGTDEHPLILRYDVSGG
                     VTSNFHAFWDVGVIELFLDTGEPNRNRSPMDYILVG"
BASE COUNT          889 a         1178 c         1483 g          921 t
ORIGIN      
        1 gacgacgggt ccaccgacga cacgcctcgg attgcgcgtc aagccggctt cgcgctggtc
       61 aggcaccctg agaacagggg ggtcgcaacg gcccgcaaca ccgctatcag gacggcacgg
      121 ggcaagttgg tggcgtcgct ggacgctgac cagatcgcgc ctcgcgattg gcttgccagg
      181 atgctcaaga atttcgaggg tagaaggagg atcgttggat gttgcggccg ggttgtggag
      241 aaacacacgg ataccatcgc cgatcgctgg cgagcggttc acatgaaatt gagcttcggc
      301 atgcggcgct cttacgatcc tcgatggctt cacggtggca cgatgctcgt tcgcgacgcc
      361 gtccttgatg tggggttata caacgagcga tgcctgcggg catacgagga cgttgaactt
      421 gtcgctcggc tgaaggcagc gggtcatgtt ctgctctacg atccgacgat catcgcgact
      481 cacctgaaga gaagccgccc tggtgacgtt gtgagggatt tctggagcta ctgggcggcc
      541 aagaacgaaa tcgagggcgc gtacaaatcg ctggcggccg cgtgtcggtt gatgatcgcg
      601 cgtcagatgg gaatcgccgc gtatcgtatc ggccaggatc tgaagcacaa gcgtgacgaa
      661 ctgcttcccc ttgacgcaat catcccgctg gctttttgcg tccgcgacct tgatgagatg
      721 gttcgcctgg gccgccttcg gcgggagcag gctttggata tccagacggc acttactcgt
      781 tgctaccagg gcgtttggga ggagctgttc ccgcgtaaat gctccggcga tgactgcata
      841 gcggcgggta tggcccgact tgcctttacc ggcggtcgcc tgcccgacgg tcgaccgaac
      901 agacttgccg ccgccgccaa aaactatgtc tcagccttca ggagagcctt tgcccatctt
      961 ctggcagacc tgcccgtacc tgtcagggaa cgtattctgc gtagggcgag cgatgtactc
     1021 ctagaggcag aaccgtcgat gagagccggt tggtcgggcg aatgattatt cggaatggtt
     1081 gcctggttcc cggcgggcat cgtgtggtag ccaagagcga accatcagga ggtcaaattc
     1141 ggactgtttt agggcgatgc cggcaggctg gtcgggggcc atcatgcggg attgatcggg
     1201 tcgccgaaca aggtggaagg tggcagcctt tgtacagatt tttctatttg gctgaattcc
     1261 agctacttgg gagctagccc ggggtccaga attacgcaat ttctggcgtc agaatgaggt
     1321 gcttttacgt cgacaaattg ttacactcag cggtatgaga acggtgaaga caaccaggca
     1381 acaaccgact aaccagatca caggccttgg ccgaatgcgc gaagagcacc agcgagacgt
     1441 ggcctacgtc cagcttcgcc accatttgat tcttcagcag attcctccgg ggtcgaggct
     1501 ggccgaagtg gagtgggcgg ctcggctggg ggtgaaccgt acggcactcc gggaggcatt
     1561 tgcacggctg gcatcggaag ggctgatcag gcgcggtgaa aagaccgggt acatcgtgcc
     1621 agtgctcacc aagaaagatg aacacgacag cctgctcgtt cgggtggcgc tggaggcctg
     1681 tgcgatcgac attatctgcg ccagcgggct gaacacgccg tcgcatctgg agcggctttc
     1741 ggcagcgtgc gacttgctgg cgcaattggt tgacgaggaa taccacctga gcacggtgga
     1801 agccgattgg cgcttccatc aggctctcat agaggcagcc ggcaacgagc cattggtcat
     1861 cgcgtaccgt cacgctccgg tattgatgct gcaggccact attcggagag ggccggactg
     1921 ggttcagcgg gaagaacgca gcattgccga gcatcgccag gttttggccg caatgctgag
     1981 cggagacgcg acagaagcga aaagagtgct gcggcagcac ctggtcgact catgggaagc
     2041 ggagcggacg agtcgcaaga cgatggagtc ggcgtaaggt tgcgggcgca gcggtccagt
     2101 gcggcgcctg cccaaagggg agaatcgttg gcagagtggg taacccgaag ggagaaggaa
     2161 ctgcgcctaa cattcttggc aagcaggaga tccgtggagc attgaggtgt gattcctaag
     2221 atcttgctag acatgtgtgt atgcccatga tgcgccgttt caatttgttc caagtcgact
     2281 attttcactt ggtttattaa gctatagatg gaaatatgcc cgggctggcg ttatcagggc
     2341 gcgcgcatac agattttccg ctttttctgt tgacagaaga gccggaatcc gatattctgg
     2401 ccaatggctg cgagaggtgc cctccggaat cgggtgtggg cagcgcatca caggtggaag
     2461 tggccccatg agcgcggtca gtcaaatccg tctatcgagc cgcgccgcag tgctggggaa
     2521 cggcatgtcg atgcgcgatc ttagtgcgta gcccaagttg atacctgtcg ggcatcgccg
     2581 gccttctgga acgtcgaacg ggttgttcaa ggtttcggta agcgggcaca caacaatggc
     2641 acgaggaggg tcaaaagtga aaaacctgtc aaatatactg gttgcctttg tggggtttga
     2701 gctgctgctg tcgccggcgg tgggaggctt cctgctggag aacggcggga tggaggaccc
     2761 ctttgccggg agttatccca atgtcgtggc cgtgccttgg acctactact catacggagg
     2821 actgagtggg gcccagcaaa gcaaggagac gacggtcgta catggtggct tatcgtcgca
     2881 gagactggcc ggcaacgcga cgaataacgc cgcttacttt ggcatccggc agacgatcga
     2941 cgcaaacgtc ggcgacgcct tcatgtttgg cggctgggtc tggccggatt cggccggcac
     3001 ttacaacgag acgtccattc gggtggcatg ggacggcgga accgctgctc tggatgccac
     3061 tgtcttaatg aactggctgc ctttgggctc gcaaaagcag caatggcatc agatggttac
     3121 gacttccggc tcgacgctgg ctggcggcaa cgcaaccgcc acgacgatca ctgtgtttct
     3181 tcatagcgac aggaacacga gcaatggcag ccttcttacg tactgggatg acgtggtcgg
     3241 ttatcacacg tttgtgcccc cggccccgat cctcgggaat cagacgccaa gcagctttga
     3301 ggtgaacgtg gacccggggg gcaactccgg caacctcttt gcccaatatg ccatcacggt
     3361 cggcggcggc ggttacacgc tgggatcgga ctggttgcag gccgatggca gcgtgggcgg
     3421 cagccctgtc tggcgaacgg acgcacagtg gggaacgacg acggtgacgg gcgtggcggg
     3481 ggacacgatc tacagcgttc aggtgaaagc acgctacgac agcgtcagag cgcaagaaac
     3541 ttacctgggc ccggtggcga tgattccgga gcctgtgacc gtcgccttgt tgggtttggg
     3601 catcgcggcg gtgattcggc ggcggtaggt gtcgttccca tggctttgcc gggttatggt
     3661 cgtattcgcc gagaagggcg acggggtgtc atatagctct gcatgcagac tcaaactggg
     3721 gatgctgaat atgcgtcggc ttgcgtcggt ggcaagccgc gaacagcgag agttggttgg
     3781 caagtgttag cggcatggcc gggatcgaga gcggcgtcag gccggcccgg tcccgcagtg
     3841 aatcgcaggg tgaagtgagg caagtttttc ttggaggatt ggatgatgaa tcgacaggtt
     3901 gttctgattc tcgcggcatg tatcggtttg atttcggcgc aggcctgggc tgacacatgg
     3961 tgcaacagct cgtacccgcc tgatccgccg gcatgtcatt ccgcggcgat ccactgcgag
     4021 gatgtggacc ggatctgcaa cggcgccccg gcctgcccgc ctcctcctcc tgaaccatgt
     4081 cccggccagg actggagctg gatgttgatt cgtcaggcgt ttccgcgcac ctcgagaatc
     4141 aatgacacgg ggtcggagtg cggggtcgag ttcaagggcg aggaggacac caaactgttt
     4201 acgtcttacc cgctcgggac gaggtatccg tacggggatc tcggccaggc caccatcgat
     4261 ttgagcggtc acatccgata caccttcggc tcggacgaat acactgcggt tgttggcacc
     4321 gacgagcatc cgctgatatt gaggtacgac gtcagcggtg gggtcacgtc gaatttccat
     4381 gctttctggg acgtcggcgt catcgagttg ttcctggata ccggtgagcc gaaccggaac
     4441 cgatcgccga tggattatat cctggttggg g
//
LOCUS       DAOTPW010000351         4450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15975_length_4450_cov_5.752901, whole genome shotgun sequence.
ACCESSION   DAOTPW010000351 DAOTPW010000000
VERSION     DAOTPW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15975_length_4450_cov_5.752901"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..681
                     /locus_tag="P5316_20625"
     CDS             493..681
                     /locus_tag="P5316_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87420.1"
                     /translation="MKLHRIYRFVLLAATGGVVFQATSSCSTEFSSTLSTALQQVIIS
                     AFEAVVRAYLNQLLYGTI"
     gene            complement(833..1666)
                     /locus_tag="P5316_20630"
     CDS             complement(833..1666)
                     /locus_tag="P5316_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRR87421.1"
                     /translation="MIRAGYRNRHGFTLIEVLVVVAILAMLIAILLPSLSNARARARR
                     TVCLSNLHQQCLGFAAYAADHKHKLPLHGGTGYSLRESDEQVWGRPRPGTRSLPVNAG
                     LLYGKYCGKEGVFFYCPSNLERNYDDPSYGWVTFKNPGPVPYLTWGGYMYAMSVRPAH
                     APSDDGTLSFTYVPGLSIYGDSWGDYKQWQEDYRATVEPPGDERVYFGKQQALLYDNP
                     QVIPHTTGHNVAFTDYHAKWVSDPHGFVKAHFPSSPDVRWDLRSFLNRCWVHSFLSRR
                     Q"
     gene            complement(1820..2125)
                     /gene="bamE"
                     /locus_tag="P5316_20635"
     CDS             complement(1820..2125)
                     /gene="bamE"
                     /locus_tag="P5316_20635"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRR87422.1"
                     /translation="MIKRIAIGAACLALIAAFCIVLSTSVFSCRHKQSGPANGLAGWS
                     ALREGMTEDQVRTVLGEPKSKRTNPMEGTFWEYTEGTRAGMVRFDPRTGRVTHWNVP"
     gene            2263..2345
                     /locus_tag="P5316_20640"
     tRNA            2263..2345
                     /locus_tag="P5316_20640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2296..2298,aa:Leu,seq:taa)
     gene            2632..4266
                     /locus_tag="P5316_20645"
     CDS             2632..4266
                     /locus_tag="P5316_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRR87423.1"
                     /translation="MEYSISPDGERFPLPSEADYAAEFERLQKIVAEQRRQGREIVVV
                     VGVGFVGAVMAAVVADTVDKATGKPSKFVIGMQRPSPRSFWKIGYLNRGIPPVKAEDP
                     EVDAIIPRCVKEKGTLVATYTNEALKLADVVVVDVQCDYIKKDLGNLVTGHTDMAALE
                     KSFEVIGRYIPPSCLVLIETTVAPGTTEYVAFPILKRAFEERKVESEPLLAHSFERVM
                     PGRNYVASIRDFWRVCSGVNEESRRRVTKFLSEVLNVEKFPLTVLDRPIESETTKIVE
                     NSFRATMLAFLDEWSLFAERNGVDLIKVVNAIKVRPTHNNIIFPGPGIGGYCLPKDGG
                     LGMWAYRHLMGFEDDIFKITPTAINVNDTRALHVAELTRDALRNMGKIVAAARITLLG
                     AAYREDVGDTRYSGSELVVRKLTEMGAEMRVHDPYVQHWWELEAQDTYPSPGHSWARF
                     FRNQERLKDIRVQKDLAEALKGADAVILAVRHKPYLELSPDDVVRMAGRPTCVIDCFG
                     ILDDNRIRRYFELGCEVKGLGRGHVKRIKDEVAARR"
     gene            complement(4238..>4450)
                     /locus_tag="P5316_20650"
     CDS             complement(4238..>4450)
                     /locus_tag="P5316_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87424.1"
                     /translation="HRAVELAAQPEHRIPRNYPNALSGQHLTPQPCWNSPCAAPSPAV
                     ATGLPKEHASLDARVGHFIVERPLRP"
BASE COUNT          825 a         1353 c         1393 g          879 t
ORIGIN      
        1 cgtccggttc ggagggaggg gggacggtca atccgtcctt cctaccccta tcttgcgggt
       61 ccgctggtcc cagattccta tctgggacca ctcttccgac gatttcgctg gtcccagatt
      121 cccatctggg acccttctgt tgacgattcc catcgtcatt ctcaccgaac ccgtcgtctc
      181 aaatttcaga tcaaccgtgc cgcgaggagg cgtccccgtg tacacgccgg tttgcgcggg
      241 tgctgcggcc gacatctttt gccgccacgg tgtcagttgc gttatccgac tgtctgtgct
      301 ttaatggcgt cgccggatct tgtggcggca tctgccgcgt gcagcggcgt tgtcagccca
      361 gactccagtc ggcagtctcg gccggtcgcg gatgccaccg gagaagatcg agtgactgag
      421 aagctgcccg tccgatgtgg gcatggctgc tgaaagacaa gagtcctttg ggccgggaaa
      481 cggagtgctt agatgaagct tcatcgcatc tacagattcg tgttgctggc ggcaacggga
      541 ggtgtggtct ttcaggccac atcgagctgc tcgaccgaat tcagcagtac actctcaacg
      601 gcgctgcagc aggtgatcat ttccgccttc gaggcagtgg tcagggctta cctgaatcag
      661 cttctgtacg ggaccatctg acgtcccccg aaagaaccgt tccattccgg gacacgcgca
      721 gacggccgga gatccggcag gcggcttggc gacggatcga gagccctgtt cccgccaacg
      781 ccgcgagctt tgacggttca tcggccgcgg acggactcag ccacgaagat ggctattgcc
      841 tccttgagag gaagctgtgc acccaacagc ggttgaggaa gctgcgaagg tcccatctca
      901 cgtcggggct gctggggaaa tgcgccttga cgaatccgtg cggatcgctc acccacttgg
      961 catggtagtc cgtgaaggcc acgttgtgcc ctgtcgtgtg gggaatgacc tgcggattgt
     1021 catacaggag ggcttgttgc ttgccgaagt agactcgctc atctccgggg ggttccaccg
     1081 tggctcggta gtcctcctgc cactgcttgt agtcgcccca ggagtcgccg tagatcgaga
     1141 ggccgggtac gtaagtgaaa ctcagcgtcc cgtcgtccga gggggcatgc gccgggcgaa
     1201 ccgacatggc atacatgtat cctccccacg taaggtacgg aaccgggccg gggttcttga
     1261 acgtcaccca tccatacgag ggatcatcgt agttgcgttc caggttgctg ggacaataga
     1321 agaacacgcc ttccttcccg cagtatttcc catagaggag cccggcgttg acgggcaaac
     1381 tgcgtgtccc cggcctcggc ctgccccaga cctgttcgtc ggattcccga aggctgtaac
     1441 cagtaccgcc gtgcagcggc agcttgtgct tatggtcggc cgcataggcg gcaaagccca
     1501 agcactgttg gtgaagattg gagagacaga cggttcggcg ggcccgcgct cttgcattgg
     1561 acaacgacgg cagcaggatc gcgatcaaca ttgccaggat cgcgacgacg acgagaacct
     1621 cgatcaacgt gaagccgtga cgattacggt agccggcacg aatcatgttc gtcacctccg
     1681 ccgattgcgg gcccgatggg cagatcctgc cgcccccgca cgccacctga ggcggaccgc
     1741 ctttttgcgg gccgcaaccc acgcgctccg cgatccagaa acatgcgcgc aagccgcacg
     1801 gatctccggc cgtaagcttt tacggtacgt tccaatgcgt gacgcgcccg gtccgagggt
     1861 caaaacgcac catgcccgcc cgcgtgccct cggtatactc ccagaacgtg ccctccatcg
     1921 ggttcgtacg tttggacttc ggctcgccaa ggacggtccg gacttggtcc tcggtcatcc
     1981 cctccctcag cgcgctccaa cccgcaagcc cgttcgcggg gccactctgc ttgtgcctgc
     2041 acgaaaagac ggatgttgac aagacaatgc agaatgccgc gatcaaggcg aggcacgcag
     2101 ccccgatggc gattctcttg atcatgttgc cagtaccctc tcatcagaat tgtcggctcc
     2161 tggcaacttg atgctaacaa accaggcggg cctctgtcaa caagaatcgc gtgcgtgcgc
     2221 caacttgagg ccgaatccca gggcactgta gaatggccgc acgggccggt ggcggaatgg
     2281 cagacgctgg ggacttaaaa tcccctgtcc gagaggacgt gcgggttcga ttcccgcccg
     2341 gcccagtctt tccggggcaa tcttcgggac agatctgagc tttgagggac cgcgtcaagc
     2401 tgcctcgcga ttgccgattc agcggtggcg agtcccctct tgccacctgg cagatgtcca
     2461 tgatctgatt cgttgccttc ctgggaatgg tttgtgtctt gccgttgcgg tgcttaccgg
     2521 cagttgaggt gaccactttg ccggccttgt cgcgccattt gaccgcgccg gccggggcgg
     2581 ttactttgcc ggtccagtca cattagcggt attgcatgcc gggaggttga tatggagtat
     2641 tcgatcagtc ccgatggcga acggtttccg ctcccgagtg aggcggacta cgccgcggag
     2701 tttgaacggt tgcagaagat cgtggccgag cagcgccggc agggccgcga gatcgtcgtc
     2761 gtcgtgggcg tgggcttcgt cggggccgtc atggctgcgg tcgttgccga taccgtcgac
     2821 aaggcgaccg gcaagccgag caagttcgtc atcggcatgc agcggccgag cccgcggagt
     2881 ttctggaaga tcggctacct caaccgcggc atccctcccg tgaaggccga ggacccggag
     2941 gtggatgcca ttattcctcg ctgcgtcaag gaaaaaggca cactggtcgc cacgtacaca
     3001 aacgaggcgc tcaagctggc cgacgttgtg gtcgtggacg tgcagtgcga ctacatcaag
     3061 aaggacctgg gcaatctggt caccggtcac acggacatgg ccgctctgga gaagagcttc
     3121 gaggtcattg ggcgatacat ccccccgagt tgcctcgtcc tgatcgagac gacggtcgcc
     3181 ccgggcacca ccgaatacgt cgcgtttccc atcctcaagc gggcatttga ggaacgcaag
     3241 gttgagtccg agcccttgct tgcccacagc tttgagcggg tcatgcccgg ccgcaactac
     3301 gttgccagca ttcgcgactt ctggcgggtg tgcagcggcg tcaacgagga aagccgccgc
     3361 cgtgtcacga agtttctgtc cgaggtgctc aacgtcgaga agtttcctct caccgtcctt
     3421 gacagaccca tcgaaagcga gaccaccaag atcgtcgaga acagtttccg ggccacgatg
     3481 ctggccttcc tcgacgaatg gagccttttc gctgaacgca acggcgtcga tctgatcaag
     3541 gtcgtcaacg ccatcaaggt ccggccgacg cacaacaaca tcatctttcc ggggcccggc
     3601 atcggtggct attgccttcc caaggacggc ggcctgggca tgtgggctta ccggcacctg
     3661 atgggcttcg aggatgacat cttcaaaatc acgccgaccg ccatcaacgt gaacgatacc
     3721 cgggcgctgc acgttgccga gctcacccgc gacgccctcc gcaacatggg caagatcgtg
     3781 gcggcggccc gcatcaccct gctcggggcg gcgtatcgcg aggacgtcgg cgatacgcgt
     3841 tacagcggca gcgagttggt ggtccggaag ctcactgaga tgggggccga gatgcgcgtc
     3901 cacgaccctt acgtacagca ctggtgggag ctggaggcac aggacacgta cccatcgccc
     3961 ggccacagtt gggctcgctt cttccgcaac caggaacgcc tcaaggacat tcgagtccag
     4021 aaggatcttg ccgaggccct caagggggcc gacgccgtga tcctcgccgt tcgtcacaag
     4081 ccgtacctgg aactctcgcc ggatgatgtg gtccgaatgg ccggccggcc gacctgcgtc
     4141 atcgattgct tcggtattct cgatgacaac cggatccgcc gctatttcga attaggttgc
     4201 gaggtgaaag gtctgggtcg aggtcatgtc aagcggatca aggacgaagt ggccgctcga
     4261 cgatgaagtg accaacacgg gcgtccaatg aagcgtgttc cttcggcaag cctgtggcaa
     4321 ccgcgggaga tggggcggcg cacggcgagt tccagcaggg ttggggagtg aggtgttggc
     4381 cgctgagcgc gttcgggtag ttcctgggta tccggtgttc gggctgggcg gcgagctcta
     4441 cggcgcggtg
//
LOCUS       DAOTPW010000352         4447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_15989_length_4447_cov_5.984973, whole genome shotgun sequence.
ACCESSION   DAOTPW010000352 DAOTPW010000000
VERSION     DAOTPW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15989_length_4447_cov_5.984973"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..506
                     /locus_tag="P5316_20655"
                     /pseudo
     CDS             83..506
                     /locus_tag="P5316_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
     gene            630..929
                     /locus_tag="P5316_20660"
     CDS             630..929
                     /locus_tag="P5316_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRR87425.1"
                     /translation="MRSQGGYRLAITFEDGVRADLDFAPLVERGGLFADLKGIAVFAQ
                     VRVDPEAETLVWPNGADICPDVLYHIATGAPSPGEVAWTAAKCVRVKLVAAGTGA"
     gene            1196..1816
                     /gene="aat"
                     /locus_tag="P5316_20665"
     CDS             1196..1816
                     /gene="aat"
                     /locus_tag="P5316_20665"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRR87426.1"
                     /translation="MKTLPLTPQVLVAAYASGIFPMDVEGRIQWFSPDPRAVIPLDGF
                     HASKTCMQTWRSGRFEIRVNTAFEEVMRGCADRREGTWISEQIVEAYCRLHELGLAHS
                     VESWRAIDQPAGSSQGSAGELAGGLYGVAIGGAFFGESMFSRQRDASKVALVALVERM
                     RSRGFALLDVQFLTPHLQRFGAVEIPRREYLERLKDALKIRASFVD"
     gene            1843..3777
                     /locus_tag="P5316_20670"
     CDS             1843..3777
                     /locus_tag="P5316_20670"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRR87427.1"
                     /translation="MSGSAPATNPLVRDEVYCRNMAALFRSDPRLAQRIDECADDDSV
                     VIEQAKTGVPTVAVRLPGSERPFYLHSRIDPETESKKWADSVEIGDSLCYIINGFGLG
                     YHVRALFSRLKGEAFIIVTEPSLPLLKAAMETMDLADLFRSDRCIILTSTDKNEVQTR
                     LEPHNTAMMMGAQFVSHAPSGRIAADFQAAIRKLLTDHMTYCRMSLVTLVTNSRITCR
                     NIANNLPRFLATPPIDVLRDRFAGYPAIVVSAGPSLRRNIDQLAALKGKAVIIAVHAI
                     YKTLLDRGVIPDFVTVLDYHELTKRFFEGVQDFSGTHMIAEPKVNWGVLDLYKGPVSI
                     LSNSFATAVLGRKLAARDGLKAGATVAHLSLYLAVYMGCDPIIFVGQDLGCTDHVYYL
                     PGVAIHDLWRPELNRFHSIEMKEWERIVRARPILIKAKDIHGNDIYTDEQLFTYLQQF
                     EGDFAALPRGRVIDATEGGVRKAGTTVMKLSETAERYCTRPIPREKFDYLRKLDWNDR
                     SRLAAGKAEVEKRLQDIDEMRQTCQHMLAVLKELTGLLDRPAEFNRRIAEVDALRVRM
                     RQLVKTYEIVSAVSQHAELQRFSADRRIAMSEHDDVSRARSQLRRDIQFVEAIVEGAG
                     VAREILSEALSRFDAVIAKE"
     gene            3997..>4447
                     /locus_tag="P5316_20675"
     CDS             3997..>4447
                     /locus_tag="P5316_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87428.1"
                     /translation="MKTIAVIEADFARGVLGTRARLNDDLLGETVLRRTLRRVLAAKR
                     VDRTHLVVHRRERHLADAAVAGLDVVVQTHDAGSVPWREMVASGRKWSLDAWRGGLVG
                     TTVFDESLNPWLIDAVCRMDHADAVVAVPSAAPLLDPDLLDRLIDHYD"
BASE COUNT          833 a         1379 c         1381 g          854 t
ORIGIN      
        1 gcctccccgc aggaggtcga acgatgtcgc cagacccatt gcctggtcat tgccccggcg
       61 ctggcggaag ggagggtggt ctatgcccct taagcgtttt ccaccggacg gccccaggga
      121 gtggctgaac cgggcccgga gcaacctggc tcgagcgaaa acggaggtcg cgggggccta
      181 tctcgaagac ctgtgcttcg atgcccaaca agccgccgag aaggcggtca aggccgtgtt
      241 tattcatcgc ggcattcgct ttccctacat ccacgacctg gcacgattac tctctctgct
      301 cgaacagggc ggctgcctga ttccagcgtc ggtcgggcga gccgggggcg ctgacgcgtt
      361 ttgcggtcga gattcggtat ccgggcctgg ctggtccggt ccggaaaaag gaataccagc
      421 gtctggtccg gaccgccgaa gcggttctcc gatgggcttc acgcgagatc gttcccgctc
      481 ccaagccaaa acggcgacgt cgttgattct tgagcaacgc gggcgatttg tcggatgcct
      541 ggaaccgcgt gcaggcgggt cagctgccgg cggggatcga gccgttggcc tgaacggaga
      601 ctcgggggat gtatccatga ccaacacagg tgcgatcgca aggtggctac cgtctggcga
      661 tcacctttga ggatggcgta cgcgcggatc tggactttgc tccattggtc gagagaggcg
      721 gcctgtttgc ggatctgaag ggcatcgccg ttttcgcaca agtgcgcgtc gatcccgaag
      781 ccgagacact ggtgtggccc aacggggcag acatctgccc ggacgtcctg taccacatcg
      841 ccaccggggc cccttcgccc ggtgaggttg cttggacggc ggccaagtgt gtccgcgtca
      901 agctggtggc ggccggcact ggtgcatgag tccaaacggc ggggcttgcg gctggtcgcg
      961 agatccggcc gtcgcgggta ctcaacgccc ctgtgttcct cgcctctcat gactgaaccc
     1021 tgaatcgtgg aaggccgacg agggcgcgcg acacggcgac acgatgtggt tgccgaagtc
     1081 cgccagaaga tcaatcggtg agtgcggacc gtgttgctca cagccaagga gaagccgtgt
     1141 cgtcgccggg tcggccctac tgaacaccga acgctgaacc ttgtatactc aaggcatgaa
     1201 gacgctcccc ctcacgcctc aggttcttgt tgccgcgtat gcgagcggca tcttccccat
     1261 ggacgtggag ggccggatcc agtggttcag ccccgatccg cgggcggtca ttccgctgga
     1321 cggctttcac gcctcgaaga cgtgcatgca gacctggcgc agcgggcggt tcgagatccg
     1381 cgtcaacacc gccttcgagg aggtcatgcg cggctgcgcc gaccggcgcg aaggcacctg
     1441 gatctcggag cagatcgtag aggcctattg ccgactgcac gaactgggcc tggctcactc
     1501 ggtcgaaagt tggcgggcca tcgaccaacc tgcggggagc agccaagggt cggcgggcga
     1561 gttggccggc gggctctacg gggtggctat cgggggcgcg ttctttggcg aatccatgtt
     1621 cagccgacag cgggacgctt ccaaggtagc cctggtcgcc ctggttgaac gaatgcgcag
     1681 tcgcggtttc gccctgcttg acgtacaatt ccttacgccg caccttcagc ggttcggggc
     1741 ggtcgagatt cctcgtcgcg agtacctcga acgcctgaag gatgcactga agatcagggc
     1801 gagcttcgtc gattgacgtg accggatcaa ggagaatggg ttttgtcggg atccgcacca
     1861 gcaaccaacc ccctcgttcg tgatgaggta tactgccgga atatggccgc cctgttccgc
     1921 agcgacccgc gacttgccca gcgcatcgac gagtgcgccg acgacgactc ggtggtcatc
     1981 gaacaagcga aaaccggggt gcccacggtt gcggtccgtt tacccggctc tgagcgccca
     2041 ttctatctgc acagtcgtat cgaccccgaa accgagtcaa agaaatgggc cgattcggtc
     2101 gagatcggcg actccttgtg ctacatcatc aacggattcg gcctgggtta ccatgtgcgg
     2161 gccctgttct cgcggctcaa gggcgaagcg ttcattatcg tgaccgagcc gagtctgccg
     2221 cttctcaagg ccgccatgga aaccatggat ctggccgacc ttttccgttc cgaccgctgc
     2281 attattctga ccagtaccga caagaacgag gttcagaccc gcctggagcc ccacaacacc
     2341 gccatgatga tgggtgctca gttcgtcagt catgcgccct ccgggcgaat tgccgcagat
     2401 ttccaggccg cgatccgcaa gctcttaacc gaccacatga cctactgccg aatgagcctg
     2461 gtcacgctgg tgaccaactc ccgaatcacc tgccgcaaca tcgccaacaa cctgccgcgg
     2521 tttttggcca ctccgccgat cgacgtgctt cgcgatcgct tcgccggcta cccggcgatc
     2581 gtcgtctccg ccgggccatc cctgagacgc aatatcgacc agcttgcagc gctcaagggt
     2641 aaagccgtca tcatcgcggt acacgccata tacaagacgc tgcttgaccg gggcgtcatt
     2701 cccgacttcg tcaccgtcct ggattaccac gaactgacca aacggttttt cgagggcgta
     2761 caggatttca gcggtacaca catgatcgcc gaacccaagg tcaactgggg cgtcttggat
     2821 ctttacaagg gacctgtgtc cattctgtcc aacagctttg cgaccgccgt gttgggccgc
     2881 aagcttgccg cgcgcgacgg tctcaaagcc ggggcgacgg ttgcccactt gtcgctgtac
     2941 cttgcggtct acatgggctg cgatccgatc atcttcgtcg gccaggatct gggatgcacc
     3001 gaccacgtct attacctgcc cggtgtcgcc atccacgacc tgtggcggcc ggagctgaac
     3061 cgcttccact ccatcgaaat gaaggaatgg gaacgaatcg ttcgagcccg gccgatcctg
     3121 atcaaggcca aggacattca tggcaacgac atctacaccg acgagcagct tttcacctat
     3181 ctccagcaat ttgagggtga ctttgccgcg ctgcctcgcg gacgggtcat cgatgccacg
     3241 gaaggcggcg tacgcaaggc cggcacaacg gtaatgaagc tctctgaaac ggccgagcgg
     3301 tactgcaccc gtcccatacc gcgcgagaag ttcgactacc tccgcaagct cgattggaat
     3361 gatcgttcgc gcctggccgc cggcaaggcg gaagttgaga agcgactgca ggatatcgac
     3421 gagatgcgcc aaacgtgcca gcacatgctc gccgtactca aggaacttac cggcctgctt
     3481 gaccggccgg ctgagttcaa tcggcgaatt gccgaggttg atgccctgcg cgtccggatg
     3541 cgccaactgg taaagaccta cgagatcgtc agcgcggtgt cacaacacgc ggagttacag
     3601 cgattcagcg ccgaccgccg cattgccatg agcgaacatg atgacgtttc ccgggcccga
     3661 agccagttgc gtcgcgatat ccagttcgtc gaggcgattg tcgaaggagc cggcgtggcc
     3721 cgcgagatac tgagcgaagc cttatcccgc tttgacgccg tgatcgccaa ggagtaaggg
     3781 tatgggagaa gagctgtcag cgatcagcga tcagcgatca gccagaaccg acagtggggt
     3841 cttgttgact cgtctttcat ccttcatcct ctatcctttc tttctgaacc ctgtcttcga
     3901 ccttgaggct gagcctcagg ctcagacccg aagggaaccc cgaaccctgc tcgccgcgct
     3961 gaagcaaagc gaagggggga aacccggtcc aaccccatga agaccattgc tgtcatcgaa
     4021 gccgattttg cacgaggggt actcggcacg cgggcccgcc tgaatgacga tctgctgggc
     4081 gagacggtcc tgcggcggac gttgcggcgc gtcctggcgg caaagcgagt tgaccgaacc
     4141 cacctggttg tgcatcgtcg cgagaggcac ctggctgacg cggccgtggc gggcctcgat
     4201 gtggtcgttc agacccatga cgccggttcg gtgccgtggc gcgagatggt ggccagcggg
     4261 cgtaaatggt ctcttgatgc ctggcgcggc ggcttggttg ggaccaccgt cttcgacgag
     4321 agcctcaatc cgtggttgat cgacgccgtt tgccggatgg atcacgccga cgccgtggtg
     4381 gccgtgccgt cggccgcacc gctgctcgat cccgatctgc tggatcgact catcgaccac
     4441 tatgacc
//
LOCUS       DAOTPW010000353         4418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16112_length_4418_cov_5.148980, whole genome shotgun sequence.
ACCESSION   DAOTPW010000353 DAOTPW010000000
VERSION     DAOTPW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16112_length_4418_cov_5.148980"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1353)
                     /locus_tag="P5316_20680"
     CDS             complement(73..1353)
                     /locus_tag="P5316_20680"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRR87429.1"
                     /translation="MNRGTKGLRRLLVASAMGAVAVVLGGCSAAQSPGGHAVITPATE
                     PKAVVMTPKGTELHRSLLVIDGHNDLPWQIRTKADSSFDKMDLRQHQESVQTDIPRLR
                     QGGVGAQFWVVYVPAETEKNGGAAKMALEQFDLIDRMISRYSDVFELARTAADVERIY
                     RQGKIAALIGIEGGHTIENSLDNLSLFYGRGARYMGLTHSETTDWADSATDEPRHGGL
                     SEFGKRVVQEMNRLGMLVDLAHVSADTMRDALRISKAPVIVSHASAHAIAAHPRNVPD
                     DVLRMIAAGGGVVMVTFFPGYAHPQGARAMAGYFEQERALKARYPDPQEFKKAWQSYR
                     KANPIPSGDVGMVVDHIDHIVRVAGIDHVGLGSDYDGVGMMPLQLENVSGYPYITQEL
                     LNRGYSVADIRKIMGENSLRVLRRAEQVARQWSK"
     gene            1845..3017
                     /locus_tag="P5316_20685"
     CDS             1845..3017
                     /locus_tag="P5316_20685"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRR87430.1"
                     /translation="MSISQKMREQMQRSSWIRRMFEDAERIKKERGAGAVFDLSLGNP
                     IEEPPSRFLESMRSAAGDPASGTHRYMNNAGYPQTRAAVAQQVSREQGVQLNENHVIM
                     SCGAAGALNVVLKAILDPGDEVIIFAPFFVEYVFYIDNHNGVARIVESDASFQPDPGR
                     LAEAITDRTKAVIINSPNNPTGVIYSGRLIREMAAVLMEASQRHGREIYLISDDVYRH
                     IVYDDAPLPAIFAEYPSSIIVSSFSKDLGLAGERIGYIAISPAVGELEALFSACVTAT
                     RVLGFVNAPALMQRSILACLDARVDMDLYRHNRAVLCDGLRRGGFRLVEPTGAFYIFP
                     QCPEPDDVACCAALRDKGVIVVPGSGFGRPGHIRLAYSVERETAVKAAAVLAEYGR"
     gene            3139..4200
                     /locus_tag="P5316_20690"
     CDS             3139..4200
                     /locus_tag="P5316_20690"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR87431.1"
                     /translation="MSTSPMQNLRELLDHGSPGWMPFTLDVGAIGGLTSPILRQFEEM
                     TGQTDPAEYFDYDFRTFSLKTAFGGDDPRSYHAGTEAGTTFDEWGIGHGSCEVEGTVG
                     RTYPPLATATSVRDIEAYPSPAIDGTVDRSPIADYHARGYPVFGYAGSIYEWSWWLRG
                     MEQFLEDLILAPALAEAIIAKVTYYTLRLALASAQAGIDVLCFYDDAGMQTGMQISPE
                     MWRRFIKPGWHTILEHLRRNAPHVRAFLHSCGNIREIIPDIVELGFDILHPIQPECMD
                     FAEVYREFGRQIALCATISSQHLFPFGTPEQVREQVRRLKELCQADRRCILCPSNKIQ
                     PETPWANVVAFAEEARADR"
BASE COUNT          831 a         1302 c         1406 g          879 t
ORIGIN      
        1 gctgcctgcc tcaactgagc gggtgagatc atccggttcc ggatatgcgg cgtgcggttg
       61 atcaccgacg tgctatttcg accattggcg agcgacctgc tcggcgcggc gcaggacgcg
      121 aagcgagttt tcccccatga ttttgcgaat gtcggcgacc gagtatccgc ggttgagcag
      181 ttcctgcgtg atatacggat agcctgacac gttctcaagc tgaagcggca tcatgcccac
      241 gccgtcgtag tcggaaccga gaccgacgtg atcgatgccg gccacgcgca cgatgtggtc
      301 gatatgatcg accaccatgc ctacgtcgcc ggagggaatc ggattggctt ttctgtatga
      361 ttgccaggct ttcttgaact cctgggggtc cgggtacctt gccttgagtg cccgttcctg
      421 ctcaaagtaa ccggccatgg cgcgtgcgcc ttgcggatgt gcgtagccgg ggaagaaagt
      481 caccatgacg acgccgccgc cggctgcgat catccgaagg acgtcatcgg gcacgtttct
      541 cggatgggcg gcgatcgcat gggccgacgc gtgcgaaacg atgacgggag ccttgctgat
      601 tcgcagggcg tcgcgcatgg tgtcagccga gacgtgggcc agatcgacaa gcatgcccag
      661 gcggttcatc tcttgaacca cgcgtttgcc gaactccgac aggccgccgt gacgaggctc
      721 atcggtggcg gagtccgccc agtcggtcgt ttccgagtga gtcagcccca tgtagcgggc
      781 gccgcgtccg tagaacagcg acaggttgtc aagggagttt tcgatcgtat ggccgccctc
      841 gataccgatc agggccgcga tcttgccttg tcgataaatg cgctcgacgt ccgccgccgt
      901 tcgtgccagt tcgaagacgt cggaatacct cgagatcatt cggtcgatga ggtcaaattg
      961 ctcgagggcc atttttgcgg cgccgccatt cttctcagtc tccgcgggga cataaacgac
     1021 ccagaattga gccccaacac ccccctgccg cagtcgcgga atgtcggtct ggacggactc
     1081 ctggtgttga cgcaggtcca tcttgtcgaa cgaagagtcc gccttggtcc ttatctgcca
     1141 gggcaaatca ttgtggccgt ctatcacaag gagagaacga tgaagctccg tgccctttgg
     1201 tgtcatgacg acggctttcg gttctgtcgc gggggtgatc accgcgtgcc ctccgggcga
     1261 ctgcgctgcc gaacagccgc caagaacaac ggcaaccgca cccatcgccg aggcgacaag
     1321 caagcgccgt aaaccctttg ttccacgatt cattgcgagt gtacctcgtg ccagctccat
     1381 gttccaagtc aaccgtgcac cccggaagca gccgttcacg taggggcgta tgcagcgaca
     1441 gggctttcgg gccgctgatg ctgcggccgg caagccgcca agcttccgga aaaggatacc
     1501 atggggccgg ctggattgcc cgtatcacag cgagtaatat gccaagtgtg cggcgagaag
     1561 gcctggcggc gcgtctcgcc gattgtctga tgtcggttgc cgaccgccgc ccatcggccg
     1621 gcagcccgag catgtgtgcg aaagtcgacg gattcgttcg acagggcaac gtcgagcctg
     1681 cctcgccacg ggcggggtcg cgggtgcgac cgggacctgc cagattccgc agcgagaacc
     1741 ggcaggtctg cgcgctgctt cgtccatagg acttcgcaag gcttggacca tgccctacgg
     1801 tcttcacgca cactgttaca actgggccgc aggagacggc agccgtgagc atttcgcaga
     1861 agatgcggga acagatgcag cgctcgtcat ggattcgcag gatgttcgag gacgccgagc
     1921 gaatcaagaa ggaacggggg gcgggtgccg tttttgattt gtcactcggc aatccgatcg
     1981 aggaaccccc ttcccggttt ctcgaaagca tgcgatcggc cgccggcgac cctgcttcag
     2041 gaacccaccg atacatgaac aacgccgggt atccgcaaac gcgtgcggcc gtcgctcaac
     2101 aggtctcgcg ggaacaaggc gttcagctta acgagaatca cgtaatcatg agttgtggtg
     2161 cggccggagc actgaacgtg gtgttgaagg ccattctcga cccgggagac gaggtgatca
     2221 tcttcgcccc gtttttcgtc gagtatgttt tctacattga caaccacaac ggcgtcgcca
     2281 ggatcgtcga gtcggacgcg agctttcagc cggaccctgg tcgcctggcg gaagcgatta
     2341 cggatcgcac caaagcggtc atcatcaaca gtcccaataa tccgacgggt gtcatctact
     2401 caggcagact gataagagag atggcggcgg ttctcatgga ggcgtcgcaa cgacatgggc
     2461 gggagattta ccttatttcc gacgatgtgt accgccacat cgtgtatgac gatgctccct
     2521 tgccggccat ttttgcggag taccccagtt cgatcatcgt cagttcgttc tcgaaggatc
     2581 tcggccttgc gggcgagcgg atcggataca tcgccatctc tccggcggtc ggcgagctgg
     2641 aggccttgtt cagcgcctgt gtgacggcga cgcgcgttct ggggttcgtg aacgcgccgg
     2701 cgctgatgca gcggtcgatt ctggcttgtc tggacgcgcg ggtggacatg gacctgtatc
     2761 ggcacaaccg ggcggttctc tgcgacgggt tgaggcgcgg aggattccgt ttggttgagc
     2821 cgacaggcgc cttctacatc ttcccgcagt gcccggaacc ggatgacgtc gcctgctgtg
     2881 ccgcactgag ggacaaggga gtcattgtgg tgccgggatc gggcttcggc cgtccgggac
     2941 atatacgcct ggcttacagc gttgaacggg agacggccgt gaaagcggcg gccgtgctgg
     3001 ccgaatacgg ccggtaaccg ccggcatcca acatagccgc agccttctcg tggcgtgtca
     3061 cgggcggctt gtcgggcggg agtaccgtgc tcacggccgg cctgctccgg gcaactcatc
     3121 aggagaggtc aacgcgagat gtccaccagc ccgatgcaga atctgcgaga gctgctggac
     3181 catggctcgc ctggctggat gcccttcacg ctggatgtcg gagcgattgg ggggctgacc
     3241 tcgccgatcc tgcggcagtt tgaagagatg accggccaga ccgacccggc cgagtacttt
     3301 gactacgact tccgcacttt ctcgctcaag accgcattcg gcggagacga tcctcgcagc
     3361 tatcatgccg gcaccgaggc gggcacaacc tttgatgaat ggggcatcgg gcacggatct
     3421 tgtgaagtcg agggtaccgt tggccgtact tatccgcccc tggcgacggc gacatcggtg
     3481 cgggatattg aagcctatcc ctccccggcc atcgacggga cggtcgatcg gtcgccaatc
     3541 gcagattatc acgcgcgagg atacccggtc ttcggctatg ccgggagcat ctatgagtgg
     3601 tcctggtggc tccgagggat ggaacagttt ctggaggacc tgattcttgc tccggcgctg
     3661 gcggaggcga tcattgccaa agtcacatat tacaccctgc ggcttgcact ggcgtcggcc
     3721 caggcgggaa tcgacgtgct gtgcttctat gacgacgcgg ggatgcaaac gggcatgcag
     3781 atctcgcctg agatgtggcg gcggtttatc aagccgggat ggcacacgat tcttgagcac
     3841 ctcagacgca acgcacctca cgtccgggcc ttcctgcaca gttgcggcaa catccgcgaa
     3901 atcattcccg acatcgtgga gcttggcttt gacatcctgc atccgataca gccggagtgt
     3961 atggacttcg cggaggtgta ccgcgagttt ggccggcaga tcgcgctttg tgccaccatc
     4021 agctcgcaac accttttccc tttcggaaca cccgagcagg ttcgcgagca ggtgcgaagg
     4081 ctgaaggaac tctgccaagc ggatcgtcgc tgcattctct gcccgtcgaa caagattcag
     4141 ccggagacgc cgtgggccaa cgttgtcgcg tttgccgagg aagctcgggc ggatcgataa
     4201 ccaggtttcc ggccggggcc ggagcactcg tccctgaaag gcctaaacca cgcttggttg
     4261 gaaaccttcg gccttcggac gatgggaatg cagcgatcac gcggcaacaa ggcaagcaga
     4321 ccgcccgccg cagtgttgcc gagtcaggga tggctgttgc tgggaagctg tctggtgcgg
     4381 atatcgcatc ggcgacgaaa agcgtaatcg tctcgtgc
//
LOCUS       DAOTPW010000354         4363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16350_length_4363_cov_6.513695, whole genome shotgun sequence.
ACCESSION   DAOTPW010000354 DAOTPW010000000
VERSION     DAOTPW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16350_length_4363_cov_6.513695"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="P5316_20695"
     CDS             complement(<1..1074)
                     /locus_tag="P5316_20695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRR87432.1"
                     /translation="MTRLRYTIDCIGTSIMAACMAFGTADPRNIRNGSVIPDEGYCDQ
                     PYMVITKDGNWLCTFTTGKGIEGQLGQHVVASISTDKGKTWSDPPIDIEPADGPEASW
                     VMPLVTPSGRVYAFYAYNGDRIDTLGDKRGIRADMLGWYVYKYSDDNGRTWSDKRYRL
                     PVRVTACDRTNDWQGKVQIMWGIGKPVVVGPSVFFGFTKLGRYMLDEGEGWFFRSDNI
                     LTEPDVEKIEWQMLPEGEHGIRAPEFGSVQEEHNLVGLSDGSLYCIYRTTTGYPCHVY
                     SRDGGKTWTRPEHAVYTPGGRRIKNPRACPRLWQAKNGKYLLWYHNHSGKDFNDRNPA
                     WVTGGVEKDGRIYWSQPEILLWDP"
     gene            complement(1175..2611)
                     /locus_tag="P5316_20700"
     CDS             complement(1175..2611)
                     /locus_tag="P5316_20700"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRR87433.1"
                     /translation="MKSYLAAMVALVGLTLPAGPVAVAEMPFIEVDPGRTTAFRERGS
                     AEPVVMVGVNYFDHQTGWAPRLWQKFDERRVRQHLAMLHDRGFNAIRVFLTYESFHRE
                     PGQVYPEGLPKFRRLLDMCRQLGIYVMPTGPDHWEGTPDWRKGDRFADEELLKADEAW
                     WKEFAGRFKDDPVLWAYDLYNEPMIGWDSPAMKAQWNDWLRREYGAVDRIATAWNRPA
                     ELVGTLGEIPVPPPRAARNDGRLYDFQRFREFVGDEWARRMAAAIRSVDQNHLITVGH
                     IQWVAPIMIGDVRHYAGFNPRSNAQYVDFTTVHFYPLDQPRPCDSPEGLAVNRVYLEA
                     VLYQCSVGKPLVIGEFNWYGGNSEAHQGWNLPPKPAEHQVEWCQTLLDVSRGRACGWL
                     NWAFADTPGSRDITRWSGLWTEDLKLKPWGKVFGQFARDTSRHPQQPREFPKWLTGSE
                     FDHRSLVTDPEAGHALRRALRAAATRPD"
     gene            2819..3127
                     /locus_tag="P5316_20705"
     CDS             2819..3127
                     /locus_tag="P5316_20705"
                     /inference="COORDINATES: protein motif:HMM:NF014638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HRR87434.1"
                     /translation="MLVLTRLPNEAILIGDDIEITVLAISGNSVRLRVSCPRMDPPVC
                     EETLRFDNLLSISGKVEVRVVSIRQEGGRVRLGVKAPRNIPIAREEVKEQSSKSQVGS
                     "
     gene            complement(3140..4306)
                     /locus_tag="P5316_20710"
     CDS             complement(3140..4306)
                     /locus_tag="P5316_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR87435.1"
                     /translation="MTSAVASKASSSRLSQADALAMYADLSIHELGRRAHAETCRLHP
                     EPYRTYVVDRNINYSNWCTAQCLFCNFKAPPQDQAVAEGSPAGYVLSFDQIGRKIQEL
                     VDIGGTQILLQGGLAPPDLLSFNWYLDLLRFIRGSFPKIHIHAFSPPEVFHFSRVFDM
                     SIRDVLLRLREAGLDTIPGGGAEILVDRVRERISRGKTRTDEYLEVMRQAHLLGMRTT
                     ITMMFGHIETIAERIEHLRRVRDLQDETGGFTAFICWTFQPVGTALARQFPMRPADCT
                     DPATTRSVVASPPDGEHLVLAGAHEYLKTLSIARLFLDNIPNLQASWVTQGPKIGQLA
                     LFFGANDMGSVMMEENVVSAAGTTYQLNEAEIRRLIADAGWQPKKRSCYYELLE"
BASE COUNT          835 a         1357 c         1317 g          854 t
ORIGIN      
        1 ggggtcccac aagaggattt ccggctggct ccagtagatg cgtccgtcct tctcaactcc
       61 gccggtcacc caggccgggt tgcggtcgtt gaaatccttg ccgctgtggt tgtggtacca
      121 gaggaggtac ttgccgttct tggcttgcca tagacgcggg caggctcgcg ggttcttgat
      181 ccgccggccg ccgggcgtgt aaacggcatg ctcaggccgc gtccatgtct tgccgccatc
      241 gcggctgtag acatgacaag gataaccggt cgtcgtgcgg tagatgcagt acaggctgcc
      301 gtctgacagg ccgaccaggt tgtgctcctc ctgcaccgag ccgaattcgg gagccctgat
      361 gccgtgctcg ccctcgggca gcatctgcca ttcgatcttc tcgacgtccg gctcggtcag
      421 gatattgtcc gagcgaaaga accaaccctc gccctcatcg agcatgtagc ggccaagttt
      481 ggtgaagcca aagaacacgc tcggaccgac gaccaccggc ttgccgatgc cccacatgat
      541 ctgcaccttg ccctgccagt cgttggtccg gtcgcaggcc gtcacgcgaa cgggcagccg
      601 atagcgtttg tctgaccatg tccgcccgtt gtcatcggag tacttgtaga cgtaccagcc
      661 gagcatgtcg gctcgaatgc ctctcttgtc gccgagagtg tcgatacggt cgccgttgta
      721 cgcatagaac gcgtacactc gccccgacgg cgtcaccagc ggcatcaccc aggaggcctc
      781 gggcccgtcg gccggctcga tgtcgatcgg cgggtcgctc caggtcttgc ccttgtcggt
      841 gctgatcgat gctactacgt gttggccgag ctgcccctca atccccttgc cggtcgtgaa
      901 cgtgcacagc cagttgccgt ccttggtaat gaccatatat ggctggtcgc agtaaccttc
      961 gtctgggatg accgagccgt tgcggatgtt gcgtgggtcg gccgtgccaa aggccatgca
     1021 ggcggccatg atgctcgtgc caatacagtc gatcgtatac cgcagtctgg tcataacgcg
     1081 cgaacctcct gaatgaatgc atggtatcag ccgcgcgacc ggccgtcccc cgtcacgtac
     1141 gcgcggcagc gggcttcgtg cgcccactct gacttcaatc cggccttgtg gccgccgctc
     1201 gcagagcccg gcgtaacgca tgcccggcct cggggtcggt gaccagcgat cgatgatcga
     1261 actcgctgcc ggtaagccat ttcggaaact cgcgcggctg ctggggatgg cgactcgtgt
     1321 cacgcgcaaa ctgcccgaac accttgcccc agggtttgag cttcagatct tcggtccaca
     1381 ggccgctcca gcgcgtgatg tccctagagc ccggcgtgtc ggcaaatgcc cagttgagcc
     1441 aaccgcacgc gcgtccacgg ctgacgtcaa gaagcgtctg gcaccactcg acttggtgtt
     1501 ccgccggctt gggcggaagg ttccagccct gatgcgcctc ggaattgccg ccgtaccagt
     1561 tgaattcgcc gatcaccagc ggcttgccca cgctgcactg gtacagcacc gcttccaggt
     1621 agacgcggtt gacggccaga ccttcgggcg agtcgcaggg gcgcggttga tcaagcggat
     1681 agaaatggac ggtggtgaaa tcgacatact gagcattcga tcggggattg aagcctgcat
     1741 aatgcctgac atcgccgatc ataatcggcg ctacccattg gatatgcccc acggtgatga
     1801 gatggttctg gtccacggag cgaatcgcgg cggccatgcg gcgggcccat tcatcgccaa
     1861 cgaattcgcg aaatcgctga aaatcgtaca accgcccatc gttgcgagcc gcccggggag
     1921 gggggacggg aatctcaccc agcgttccta ccagctccgc cgggcggttc catgcggtag
     1981 caatcctgtc aaccgcaccg tactcccgcc gaagccaatc gttccactgg gccttcatcg
     2041 cgggagagtc ccagccgatc atcggctcgt tgtaaaggtc gtatgcccac aacaccgggt
     2101 catctttgaa ccgccccgcg aactccttcc accaggcttc atccgctttc agcagctcct
     2161 catccgcaaa ccggtcgccc tttcgccagt cgggtgttcc ctcccagtgg tccggccccg
     2221 tcggcatcac gtaaatgccc agttgccggc acatgtccag cagtctgcgg aacttgggca
     2281 gcccctcggg ataaacctgc cctggctcgc ggtggaatga ctcgtaggtc agaaaaaccc
     2341 ggatggcgtt gaaaccccga tcatgcagca tcgccaggtg ctgccggaca cgcctttcat
     2401 cgaacttctg ccacagtctg ggggcccacc cggtctggtg atcgaagtag ttgacgccga
     2461 ccatcacgac cggctcagcg ctgccacgct ccctgaaggc ggtcgtccgg cctggatcaa
     2521 cttcgatgaa aggcatttcc gcaaccgcca ctgggccggc ggggagggtc aaccccacca
     2581 aagcgaccat ggcagcaaga tatgacttca tggatcgcct cctctcgcgt ccgaaggaaa
     2641 cgctcatatc cggtggacaa gcaacggacg cacattgtca atgaaggatt cccaaatggc
     2701 cagagcctgg cgaccgacaa tccggttttt cagttgaaca cagcatccgt atcgggtacg
     2761 atttgggtcg gcgcggcggt ggatgcccaa atcgatccac tcaaggagtc gacaactcat
     2821 gctcgttctg acacgcctcc ccaacgaggc catcctgatc ggcgatgaca tcgagatcac
     2881 ggtcctggcg atcagcggga actcggtccg gctgcgggtc agctgtccgc gtatggatcc
     2941 gcccgtttgc gaggagaccc tgcgattcga taatctcttg agcatcagtg gaaaggtgga
     3001 agtacgcgtg gtttccattc gccaggaggg tggtcgggtg cgactcggcg tgaaagcgcc
     3061 gaggaacatt ccgatcgcac gagaagaggt taaggagcag tcctcgaaga gccaagtcgg
     3121 ttcgtaaccg acctggacgt cattccagca gttcgtaata gcagctgcgt tttttcggct
     3181 gccagcccgc atcggcgatc agacgccgga tctcggcctc gttcagttga taggtcgtgc
     3241 cggcggccga gacgacgttt tcttccatca tcaccgatcc catgtcattg gccccgaaga
     3301 acagggccag ttgcccgatc tttgggccct gggtgaccca actggcctga agatttggga
     3361 tattgtcgag aaacagtcgg gcgatcgaca gagtcttgag gtactcatgt gcaccggcca
     3421 gaaccaggtg ttccccgtcg ggcgggctcg ccacgacgga gcgcgtcgta gccgggtcgg
     3481 tgcaatcggc gggtctcatg gggaattgtc gagccagagc cgtcccgacc ggctgaaatg
     3541 tccagcagat gaaagccgtg aagccgccgg tttcatcctg caggtcgcgg acgcgccgca
     3601 ggtgctcgat tcgctcggca atggtctcga tatgcccgaa catcatggtg atggtcgtac
     3661 gcatgccgag caagtgtgcc tgacgcatca cttcgagata ctcgtccgtt ctcgtcttgc
     3721 cgcgactgat gcgctcccgc acgcgatcaa ccagaatttc cgccccgccg ccgggaatgg
     3781 tgtccaggcc ggcctcgcgc agcctcagca gcacatcgcg gattgacatg tcaaagacgc
     3841 gcgaaaaatg aaagacctcc ggcggactga aagcgtgaat gtgaatcttc ggaaaactcc
     3901 cgcgaatgaa tcgcaggaga tccaggtacc agttaaagct caacagatcc ggcggggcca
     3961 agcccccttg aaggagaatc tgagtgccgc cgatgtcgac cagctcctgt atcttgcggc
     4021 cgatctggtc gaagctgagg acgtagcccg cgggtgaacc ctctgcgaca gcctggtctt
     4081 gtggaggggc cttgaaattg cagaacaggc actgggccgt gcaccagttg gagtagttga
     4141 tgttgcgatc gacgacgtag gttcgatacg gttccgggtg caaccggcag gtctcggcgt
     4201 gggcgcgccg tcccagctca tggatcgaga ggtcggcgta catcgccaag gcatcggcct
     4261 ggctcaggcg agaggaagag gctttgctcg caacagccga ggtcatttca ccaacgctcc
     4321 gggctgtggg atcagccctt ctgccctggc caattcgagg aac
//
LOCUS       DAOTPW010000355         4359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16368_length_4359_cov_5.456552, whole genome shotgun sequence.
ACCESSION   DAOTPW010000355 DAOTPW010000000
VERSION     DAOTPW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16368_length_4359_cov_5.456552"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1576)
                     /locus_tag="P5316_20715"
     CDS             complement(53..1576)
                     /locus_tag="P5316_20715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRR87436.1"
                     /translation="MAASNVLVLYNGASAAAESIPTLGNDEFRSAVIDGVERNGRLAA
                     MMATPSQDGRIRLLAAVAHGNRGSLAVCGTVVDDSYAALTPECPAAHWFEREIAEQWG
                     VRPEGHPWLKPIRFHKSYRPGRDAWGRGISTVIEPCVTDYFAMDGEEVHEVAVGPVHA
                     GIIEPGHFRFQCHGEDVYHLEISLGFQHRGVERALIGGPGLRTIHYAETLAGDTSIGH
                     ATACSELLEALSGVRVPARAQIIRGIALELERLANHTGDLGALAGDVGFLPTASYCGR
                     IRGDFLNMTALICGSRFGRGLVRPGGVAFDLEDSRRAQLLERLDAARQDVTTAVDLLW
                     DSPSVLARFQETGRVDRETAVDLGLVGVAARACNIERDVRHEFPTGIYRLAHIPVSMA
                     EAGDVFARAFVRWLEIQRSLAFIQDQLRSLPSGGNRAAAKPLGRSRIAVSLVEGWRGE
                     ICHMAMTDEAGRLAAYKIVDPSFHNWMGLAMALRNQQISDFPLCNKSFNLSYCGHDL"
     gene            complement(1576..3051)
                     /locus_tag="P5316_20720"
     CDS             complement(1576..3051)
                     /locus_tag="P5316_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRR87437.1"
                     /translation="MVLALFLIPALAGIAAFFIRPHRPRRALLPAVAVAHASLTSLAW
                     LRLPAPALQGLLKLDELGLLFLSITSALFLVASFYAVGYLKRETHGQRSDFEQGFLFD
                     NIPEAVFVGCLLLFLAMMTLVSLSAHVGLMWVAVEATTLASAPLIYFHRHHRSLEAAW
                     KYLLICSVGIALALLGIFFLAVAAADVAGTATSLLTDDLVRRGAALNRPWLSAAFVFL
                     LVGYGTKMGLAPLHTWLPDAHSESPSVVSALLSGALLNCAFLGILRAHQVLTAAGCTA
                     LGQELLVLLGLISMGTAAVFIIGQQDYKRLLAYSSVEHIGILALGVGLGGAGVFGSML
                     HAVNHSLTKAMLFLVAGNLLAVYNSKSCRDVRGALRVMPFSAALWIAGFLAITGSPPF
                     GPFLSEFTVLKAALDQGRAVVAILYLGCLGIIFVGMASVFARMTQGQASLPMDQPRAH
                     EPLLSVIPPAVLGVLVLCLGVHVPTPLNSLIQRAAQMVGGS"
     gene            complement(3056..3700)
                     /locus_tag="P5316_20725"
     CDS             complement(3056..3700)
                     /locus_tag="P5316_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRR87438.1"
                     /translation="MQAYTDLVLITVALTNLALTGVASLGTCIRVMALQGVLLGIVTV
                     TVRQEGVSIHTLVFVTIAVVLKGFVFPRLLLRAVREADVRREVEPFVGFTFSLLIGTL
                     ALPAAMWLGARLPAATPQAPPLAAPVAFHAILVGLFLIVSRRKAITQVLGYLAMENGI
                     FLFGVGLVRQATLLVELGILLDVFVAVFVMGIAIFHISREFDHMDTDRLSSLKE"
     gene            complement(3702..>4359)
                     /locus_tag="P5316_20730"
     CDS             complement(3702..>4359)
                     /locus_tag="P5316_20730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRR87439.1"
                     /translation="RAVIAFPGDLVLLAYLLGLLRFLTVIGALDTGSSFEGMGASREV
                     HFSSLAEPVLMLAVAALARKLDSISLSGIYMPLSFGTWLAAGPALVMAAVAMLVIVLA
                     ENARIPVDDPNTHLELTMIHEVMVLDYSGPDLAFILYGAALKLWVFGSLLVGLCVPVR
                     TGWFVVDAGAFLLGMFALAVIVGIIESAMARLRLLRVPQLLVGAGALAVLALVLVARS
                     "
BASE COUNT          952 a         1413 c         1330 g          664 t
ORIGIN      
        1 ggtccaacaa cccgaaaaaa atctagaatc gccctttggt gttcaacttg ggctagaggt
       61 cgtgtccgca gtaggacagg ttgaagctct tgttgcacag tgggaaatcg gagatctgct
      121 ggttgcgcaa ggccatcgcc agccccatcc agttgtggaa agacggatca acgatcttgt
      181 acgcggccag tcgcccagct tcatcggtca ttgccatgtg gcagatctcg ccccgccatc
      241 cctccaccag gctcacggcg attcgagacc ggcccagagg cttggccgcc gcccggtttc
      301 cccctgacgg cagtgaacgg agttggtcct gtatgaaagc cagcgaccgc tgaatctcaa
      361 gccatcgaac gaacgcccgg gcgaacacgt ctcccgcctc agccatcgag acgggtatgt
      421 gcgcgagcct gtatatgccc gtggggaatt cgtggcggac atctcgctcg atgttgcacg
      481 ctctcgcggc cacgccgacc agtccaagat caaccgctgt ttctcgatcc acgcgtcccg
      541 tctcctggaa ccgggcgagt accgacgggc tatcccagag cagatccact gccgtggtga
      601 cgtcttgccg ggcggcatcg agcctttcca gaagctgggc ccgccggctg tcctcaagat
      661 caaacgcgac gccgccggga cgtacgagcc cgcgtccgaa gcggcttccg cagatcaggg
      721 ccgtcatgtt gaggaaatcg ccgcgaatcc ttccgcaata cgacgcggtg ggcaggaaac
      781 cgacgtcgcc ggccagcgca ccaagatcgc cggtgtgatt cgcgagccgc tcgagctcca
      841 gcgcgattcc gcgaatgatc tgggcgcgag caggcacacg gaccccgctc agagcttcga
      901 gtagttcact gcaggccgtt gcgtggccga tcgaagtgtc accggccagt gtctcggcgt
      961 agtggatcgt ccgcagtccc ggtccgccga tcagagctcg ctccacgccg cggtgctgaa
     1021 atcccaggga gatctcaagg tgatagacgt cctcgccatg gcattgaaac cggaagtgtc
     1081 ccggctcgat gatcccggca tgtaccggcc cgaccgcgac ttcatggacc tcctcgccgt
     1141 ccatcgcaaa gtagtcggtg acgcaaggct cgatgacggt cgaaatgcca cggccccagg
     1201 catcgcggcc cggccgatat gacttgtgaa agcggatcgg tttgagccag ggatgcccct
     1261 ccggccgcac tccccactgc tcggcaatct cccgctcgaa ccagtgggcg gcaggacact
     1321 cgggtgtcag tgcagcgtac gagtcgtcca cgaccgtgcc gcacaccgcc aggctgcccc
     1381 ggttcccgtg agcaaccgct gccagaagcc gtattcgtcc atcctgggac ggcgtcgcca
     1441 tcattgccgc caaccggccg ttccgctcca cgccgtcgat caccgcactg cgaaactcgt
     1501 cgttccccag tgttgggatg ctttccgcgg cggccgaggc gccgttgtac aagaccagta
     1561 cattgcttgc ggccatcagg accctcccac catctgtgcg gcccgctgga tcaggctgtt
     1621 taacggcgtc ggaacatgaa caccaagaca caagacgagc acgcccagga ccgccggggg
     1681 tatcacgctt aacaagggct catgggcccg cggttggtcc atcggcagac tcgcctgtcc
     1741 ctgcgtcatg cgtgcgaaga ccgaagccat gccgacgaat atgatcccca gacagcccag
     1801 gtacagaatg gccacgaccg ctcgcccctg gtccaaggcg gctttgagga ctgtgaactc
     1861 gctcaggaac ggcccgaaag gcggtgaccc ggtaatggcc aggaaccccg caatccagag
     1921 tgctgccgaa aacggcatga ctcgcagtgc gccgcggaca tctcggcagg acttggagtt
     1981 gtagaccgcc agcagattgc cggccacgag aaacagcatc gccttggtca acgagtgatt
     2041 gaccgcgtgc agcatcgagc cgaacacgcc agcccccccc aggccgactc ccaaggcaag
     2101 gatgccgatg tgctcgacgc tcgagtaggc cagcagacgc ttgtagtcct gttgaccgat
     2161 gatgaacacg gcggcggtgc ccatggaaat cagccccaac agtaccagca gttcctgtcc
     2221 gagggcggtg cagccagccg ctgtcagtac ctggtgtgca cgcaagatgc ccaggaaggc
     2281 gcaattgagc agggctcccg acagcagggc ggacacgact gaaggcgact cgctgtgcgc
     2341 gtcgggaagc cacgtgtgca gcggagccag gcccattttg gtgccgtatc cgaccagaag
     2401 aaagacgaac gccgctgaaa gccacggtcg gttcagtgcg gcgcctcggc gcaccagatc
     2461 atccgtcaga agcgaagtgg cggttcccgc cacgtcggct gccgcgacgg ccaggaagaa
     2521 gatgcccagc agcgccaggg cgatacccac cgaacagatg agcagatact tccaggcggc
     2581 ctcgagggag cgatggtgcc gatggaagta gatcaggggc gcactggcca gcgtcgtcgc
     2641 ttcaacggcc acccacatca ggccgacgtg ggcactaaga ctcaccagag tcatcatggc
     2701 caggaacaac aacaggcagc cgacgaagac ggcctctgga atgttgtcga agaggaagcc
     2761 ctgttcgaag tccgatcgct ggccgtgtgt ttcacgcttc aggtacccca ccgcgtagaa
     2821 cgaagcaacc agaaaaaggg cgctcgtgat gctcaggaag agcaggccca gctcgtccag
     2881 cttgagcagg ccctgaaggg ctggcgcggg caatctcagc catgcaaggc tcgtgagcga
     2941 cgcgtgagcc accgccacgg ccggcagcag ggctcgtcgg gggcggtgcg gccggatgaa
     3001 gaatgcggca atgccagcca gagcaggaat taggaagagc gccaaaacca tgctttcatt
     3061 ccttcaacga ggacagccgg tcggtgtcca tgtggtcgaa ctcccgactg atgtggaaaa
     3121 tcgcgatccc catgacgaac accgccacga aaacatccag aagaatccca agctcgacca
     3181 gcagcgttgc ctgacgcacc agcccgacgc caaacaagaa gatcccgttt tccatcgcca
     3241 ggtagccgag gacctgggtg atggccttgc gccggctgac gatcaggaac aagccgacga
     3301 gaatcgcgtg gaacgcgacg ggcgcggcca gcggcggggc ctgcggtgtg gccgccggca
     3361 gacgtgcgcc cagccacatg gctgccggca gagccagcgt cccgatcagc aaggagaacg
     3421 tgaaaccgac gaacggctcc acctctcgcc gcacgtctgc ctcgcgtacc gctcgcagga
     3481 gcaaccgagg gaaaacaaaa cccttgagca caaccgcgat ggtcacgaat accaacgtgt
     3541 gtatagagac gccttcctgt cgaacagtca ccgttacgat gcccaaaagc acgccctgca
     3601 gcgccatgac gcggatgcag gtgcccagag aagcaacgcc ggtcagggcc aggttagtca
     3661 aggcaacggt aatcaaaacc agatccgtgt aggcttgcat cctatgacct cgccaccagc
     3721 acaagcgcga gaaccgcaag ggctccggcc ccgaccagca gttgcggcac tcgcagcaaa
     3781 cgaagccggg ccatcgccga ttcgatgatg cccacgatca cggccaacgc gaacatgccc
     3841 agcagaaaag cgccggcatc aacgacgaac cagcctgttc gcacgggcac gcacaatccg
     3901 acgagcagcg agccgaacac ccacaatttg agcgcggccc cgtagagtat gaacgccagg
     3961 tcgggcccgc tgtagtccag aaccatgact tcgtggatca tggtgagttc aaggtgcgtg
     4021 ttggggtcat cgacggggat ccgcgcgttc tcagcgagga caatgaccag catcgcgacc
     4081 gcggccatca ccagcgccgg gcccgccgcg agccacgttc cgaacgagag gggcatgtag
     4141 atccccgaga gcgaaatact gtccagcttg cgggccagag cggccactgc gagcatcaat
     4201 accggctcgg ccagcgacga gaaatggacc tctctgctgg cgcccatgcc ctcgaagctc
     4261 gatccggtgt ccagggcgcc gatcacagtg agaaatctca acaggcccaa gaggtaggcc
     4321 agcaatacga gatcgccggg gaaggcgatg acggccctg
//
LOCUS       DAOTPW010000356         4318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16557_length_4318_cov_5.469857, whole genome shotgun sequence.
ACCESSION   DAOTPW010000356 DAOTPW010000000
VERSION     DAOTPW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16557_length_4318_cov_5.469857"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..2571)
                     /locus_tag="P5316_20735"
     CDS             complement(313..2571)
                     /locus_tag="P5316_20735"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRR87440.1"
                     /translation="MTAAWMSKPAEGHVAKPAQVELVLSQVDSLPTLPAVATRLLEVT
                     TSEASSARQVIELVESDQGLSARLLSMVSKASVGTPVNTVERAVVLLGFHAVRSLVLS
                     IQVFETFSQRIEESVTRADHAGFWKHSLATACAAKMLAEKAPCLSGPTLVNPEEAFVC
                     GLLHDIGKVVLNACFAKSYKRVLAKVEATRGCIADVEREVFGLDHTAAGCRLAAHWKL
                     PEMIRECIWLHHQTPASTPTRIRFPEHVRQVQVANRLAREMRIGFSGNYGPDRPLAAV
                     AESAGFDKAVLDKVCADLPELIEARAELIGLNRLTSRDVYYEALAKANAELARVNLSL
                     STANRRLEQRSRCFEALRKLTEELGHEPGHEAICRAAAGVTEMVTDCRPAAVFAVSPG
                     RSVVIIATSNGGEGGPQVEILPIAALGDVSAVSGGGEGWQNATAVPGPLLDRLRHSFG
                     SSPGWYWPIRHQDGFLGAIVIGTQRPVDLDESMSALGDALGAWLAGAESRVLSRQLNE
                     ELADLNRRLVNSQEELARARSLAMVGEMAAGAAHELNNPLAVISGRAQMLHQAEVDEE
                     TRRVALLIAEHAHRASAIVSELMQFAKPTPPRIADFSIAGLLGQLRREWLEKTSLTES
                     QFRLELSDGLPPIHADASQIKTLFDELIRNAVDALRDTPEPRLFINCRGDMTDDRLVV
                     AVEDNGCGMTPEVLEHATDPFFSRRPAGRGRGLGLSRAVRYAEINGGRIRLTSTAGQG
                     TVVLVELPVAAG"
     gene            complement(2677..3270)
                     /locus_tag="P5316_20740"
     CDS             complement(2677..3270)
                     /locus_tag="P5316_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR87441.1"
                     /translation="MPVDKEEKIGPKGVYTTGEVAEICKVSQQTVIRCFDSGRLKGFR
                     VPGSKFRRIPRESLIQFMKENGIPLDNLDSGKKRVLVVDDDEAILEMLTELLERDGRF
                     EVRVARGGYEAGLTTQEFRPDCIVLDYKLPDIDGNRVCKAVRSNPAFADMKIILISGV
                     ADPDEINELLASGADDFLRKPFQIDELISRILKLVQV"
     gene            complement(3663..>4318)
                     /locus_tag="P5316_20745"
     CDS             complement(3663..>4318)
                     /locus_tag="P5316_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRR87442.1"
                     /translation="NVFAGLRPADAGLEAGPATRAEAITPSRTCLENSPLPAEPLVQP
                     ETMDPFSRAIEAVRSAQSVIIVPGYGMALADAQETTVRLAERLASMGKSVLFAIHPVA
                     GRMPGHMHVLLAEAEVDYGMIRELKDVNPQFAQTDLALIVGACDVVNPAAIESQGTPI
                     SGMPILLAHEAGTVIVCNLDDRPGYSGVENPLYRQPRTILLLGDAKETLARLFRDLV"
BASE COUNT          819 a         1359 c         1307 g          833 t
ORIGIN      
        1 cgtccgaggc ggtggtgacg tgggtcacct cggcccagga ggtgtctcgc aggcagcgcg
       61 agaccatttc cgcgagttct tcgtcggcgt tgaccaggag aatctgcacg cgcttgtcgg
      121 tcattgctct tcccactgcc ggatcagcac caggctcgca ggttcaaaaa aggccggcgg
      181 acgcttcggc cgcgagagcg aggaggagga gaaaagagga ggaggtagcc tacacgtccc
      241 gcccggcccg gaacttctgc tgcgatatct gcttcgatca ccccgttcat attcgccggc
      301 ggttcgcggg cctcaacccg cggcgaccgg cagctccacc aagaccaccg ttccttggcc
      361 cgcggtgctg gtcagccgga tccttccccc gttgatctcg gcgtaccgga ccgcccggct
      421 caagcccagc cctcgccccc gcccggcggg ccggcgagag aaaaacggat cggtcgcgtg
      481 ctcgaggact tcgggggtca tgccgcaacc gttgtcctcc accgcgacga caagcctgtc
      541 atcggtcata tctccccggc aattgatgaa caagcgaggt tccggggtat cacgcagagc
      601 gtcgacggca ttgcggatca gctcgtcgaa taatgtcttg atctgggaag catccgcgtg
      661 gatcgggggc aggccgtccg atagttcaag gcggaattgg gattcggtca gggacgtttt
      721 ttcaagccat tcgcgtcgaa gctggcccaa taaaccggcg atggaaaaat cggcgatccg
      781 cgggggtgtg ggcttggcga attgcatgag ctccgagacg atagcgctgg cccgatgtgc
      841 gtgctcggcg ataagtaggg cgacgcggcg ggtttcctcg tcgacctcgg cctgatggag
      901 catctgagcc cgcccggaga tgaccgcaag cgggttgtta agctcatgcg ccgcgccggc
      961 tgccatttct ccgaccatgg ccagcgaacg agcacgggcg agttcctcct gggaattgac
     1021 cagccggcgg ttgagatcgg ccagttcttc gttcaattga cgcgacagga cgcgggactc
     1081 ggcaccggcc aaccaggcgc ctagagcatc ccccagggcc gacatcgact cgtccaggtc
     1141 aaccggccgc tgagtgccga tgacgatcgc ccccagaaaa ccatcctggt ggcgaatggg
     1201 ccaataccac ccgggggagc ttccgaacga gtgcctcaga cgatccagca gcggccccgg
     1261 aaccgccgtc gcgttttgcc agccttcgcc gccgccggac acggcagaga catcgccgag
     1321 ggccgcgatc ggcaggatct cgacttgcgg tccgccttcc ccgccgtttg aggtcgcgat
     1381 gatcacaacg gagcggccgg gcgacacggc aaacaccgca gcgggtctgc agtccgtcac
     1441 catttcggtc acccctgccg ccgctcggca aatggcttcg tgtcccggtt cgtggcccag
     1501 ttcctcagtc aactttcgca gggcctcgaa acaacgggac ctttgttcaa gcctccggtt
     1561 ggccgtcgaa agcgacaggt tgacgcgtgc cagttccgca ttggccttgg cgagcgcctc
     1621 gtaataaaca tccctggacg tgagacggtt aaggccgatc aactccgccc ttgcttcgat
     1681 caactcgggc aggtcggcac ataccttatc gagcacggcc ttgtcgaatc ccgcgctctc
     1741 ggccacggcg gcaagaggac gatccggccc gtagttgccg gaaaagccga ttctcatctc
     1801 ccgagcaagt cgattggcga cttggacctg gcgaacgtgc tccgggaagc ggattcgcgt
     1861 aggggtggag gcaggcgtct ggtgatgcag ccagatgcac tcacgaatca tctccggcag
     1921 cttccagtgg gccgccagcc gacaaccggc cgcggtatgg tcgagtccga aaacctcgcg
     1981 ctcgacgtcg gcgatgcaac ctcgggtcgc ctcgaccttg gccagcaccc gtttatagct
     2041 cttggcgaag caggcgttca gtaccacctt gccgatgtcg tgcagcaggc cgcatacaaa
     2101 ggcctcctcg ggattgacca gagtcggccc cgacaggcaa ggggccttct ccgccagcat
     2161 cttggccgcg cacgcggtag caaggctgtg cttccagaag ccggcgtgat cagcgcgggt
     2221 gaccgattcc tcgatccgct gggagaaggt ctcgaacacc tgaatggaca gcacaaggct
     2281 gcggacggcg tgaaagccca ggagcacgac cgcccgttcg acggtgttca ccggcgtacc
     2341 gacgctggcc ttggacacca tggaaagaag gcgggcggaa aggccctgat ccgattcgac
     2401 gagctcgatg acttggcgag cgctggacgc ttcgctggtg gtgacctcaa gcagtcgggt
     2461 tgccacggcg ggcaaggtcg gtagagaatc gacctgggac aggacaagct cgacctgggc
     2521 cggcttggcg acgtgcccct cggctggttt cgacatccaa gcagcagtca tgtgtcccct
     2581 cagcgttccc ttagtgacat cctttccctc tccagaggcg ccggtcggca tttgccggcc
     2641 cccgggtgga cagccgatca gtcgccccgg cacattctag acctgaacca acttcagaat
     2701 ccgcgaaatc agctcgtcga tctggaacgg cttacgcaga aaatcgtcgg cgccggaagc
     2761 cagcagttca ttgatttcgt ccgggtctgc cacgccactg atcaggatga tcttcatgtc
     2821 cgcgaaggcc gggttcgacc gtacggcctt gcagacgcgg ttgccgtcga tgtccggaag
     2881 cttgtagtcc agcacgatgc aatccggtcg gaactcctgg gtggtcaaac cggcctcgta
     2941 gccgcctcga gcaacccgga cttcaaagcg gccgtcgcgt tccagcaact cggtcagcat
     3001 ttcgagaatg gcttcgtcat catccaccac cagcacccgc ttcttgcccg aatcgagatt
     3061 gtccagcggg ataccgttct ctttcatgaa ctggatgagg ctttcccgcg ggatacgccg
     3121 gaacttcgat cccggcacgc ggaagccctt gagacgaccg ctatcgaagc accggatgac
     3181 agtctgctgg ctgaccttgc agatctcggc aacctcaccg gtcgtataaa cgcccttggg
     3241 tccgattttc tcctctttat ccaccggcat cttcccgttc tcccgagtca tcccattcca
     3301 caataacacc caaattcccg catccggcca aagtttccta tcttagccat cttcactgca
     3361 tactctacaa ttccgcgttg ctcgtgtcaa accgaatttt cggagcttcc ggaaccaagt
     3421 ccgatcagac tggtagtgct gataaaggtg cccggctggt gttaagaata acctttcgtg
     3481 agggtgtcac cttcccgtct gcttgctttt tcggggcaat tcggtttgcg ttatccggtc
     3541 gtggcttatg ggcaaggtgg aattatttgc ggcaattcgt tgccttctgc cagccgactt
     3601 gctcgggcgc tcgcctgtcc ttaccagttc ggttcgagcc gctcaaaaag ccgaagtacc
     3661 ggttagacaa gatcgcggaa aagacgggct agcgtctcct tggcatctcc caggagcagt
     3721 atcgtgcggg gttgccgata gagcgggttc tcgacaccgg agtaacccgg tcgatcatcg
     3781 agattgcaga cgatgactgt gccagcttcg tgggccagca gaatgggcat acccgagatg
     3841 ggtgttcctt ggctctcaat ggccgcggga ttgacgacgt cgcaggcccc cacgataagt
     3901 gccagatcgg tttgggcaaa ctgcgggttg acgtccttta actcacggat catgccgtag
     3961 tcgacctcgg cttcggccag caagacgtgc atgtgccctg gcatgcgccc ggcaacaggg
     4021 tggatggcga acagcacgct cttccccatg ctcgccaagc gctccgccag tcggaccgtc
     4081 gtctcctgag cgtcagccag agccatgcca taacccggaa cgatgatgac ggattgggcg
     4141 ctgcggacgg cttcgatagc ccgagaaaaa ggatccattg tttcaggctg aaccaatggc
     4201 tccgcgggga gcggcgaatt ctcaaggcag gtccggcttg gggtgatcgc ctcagccctg
     4261 gttgcaggtc cagcttccag tcccgcgtct gccggacgca gcccggcgaa gacattcg
//
LOCUS       DAOTPW010000357         4312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16587_length_4312_cov_6.192859, whole genome shotgun sequence.
ACCESSION   DAOTPW010000357 DAOTPW010000000
VERSION     DAOTPW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16587_length_4312_cov_6.192859"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            604..1842
                     /locus_tag="P5316_20750"
     CDS             604..1842
                     /locus_tag="P5316_20750"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRR87443.1"
                     /translation="MGSPFCTIPEAMDELKAGRFVVLVDDERRENEGDLICAAELITP
                     EMVNFMLKEARGVLCVALPRDLCRELDLQPQTTENTATLGTAFTVTIDAHPRFGVTTG
                     VSAADRARTIRRLTEPDVTEDDFCRPGHVNPLMAREGGVLVRAGQTEGTVDLLRLAGM
                     CPVGALIEVMNDDGTMARVPDLVRFCERHKLKMCTIADLIEYRHQREMLVERVETIHF
                     PTPYGRFTLHAYRSLVDIEPHLALCCGGVGERDATGRPVLHEEPVLVRVHSECLTGDV
                     FHSWRCDCGDQLHQSMRMIQEQGKGAIVYLRQEGRGIGLHNKLKAYRLQEQGLDTVEA
                     NEHLGFPADKRDYGIGAQILRDLGLRRLRIMTNNPKKISRLELYGIEIVEQVPIIIPP
                     NEVNRAYLRTKKEKMGHLLE"
     gene            1979..2275
                     /locus_tag="P5316_20755"
     CDS             1979..2275
                     /locus_tag="P5316_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87444.1"
                     /translation="MSYVLCTPPEGSSYENSIYATADVQSFKCEVTFTADTFARSFVR
                     VDDVFYVGLGCERPGENGPKPSTSTGDILRVVLNRWSSGLQSDDGESILIVEFW"
     gene            2477..3502
                     /locus_tag="P5316_20760"
     CDS             2477..3502
                     /locus_tag="P5316_20760"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF002727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRR87445.1"
                     /translation="MLTREIARILDANLNRTREALRVMEDYVRFVLDDPAGCAVLKQL
                     RHDLRECVRHVSLEDLLGARDTPGDVGTGISTESERHRTDARDACVASAKRLSEALRT
                     IEEYAKTFDVGLSAAMESLRYRAYEVEQRVIMRGPRSARFARVRLYVLVTASMCRRDW
                     LETAAEAIAGGADCIQLREKGMDDGELLVRARRLSALCRERAVVFIVNDRPDIAVLSD
                     ADGVHLGQTDMPVADARRILGPDRLIGLSTHTPEQFRAALRLTPDYIAVGPMFASATK
                     PQEHVPGPSLLALAESETSIPVVPVGGITVDNMGILRQAGARRLCVCSTVISSDDPRT
                     VARALLG"
     gene            3516..4001
                     /locus_tag="P5316_20765"
     CDS             3516..4001
                     /locus_tag="P5316_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87446.1"
                     /translation="MGFTWDELDQLAPKDKWRLPLPPTCAKCQYDLTGLPEERCPECG
                     TPFRWEEVRRRTRRIWNVALRLRHANQDAKLGITIGLIVWCALGFVRLVGLDGLAPLV
                     SIIAFGGGVISIVLGSQVLNIRRMPKWARPYVGHPPPSMLLGAGSMFLGVSQMVGAVV
                     F"
     gene            complement(4027..>4312)
                     /locus_tag="P5316_20770"
     CDS             complement(4027..>4312)
                     /locus_tag="P5316_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87447.1"
                     /translation="VSRFSFLTNDLQPRPPRNLDPAWSSAVVRLLRRVIPVGFAWPRS
                     GHRPDVLPVNSYPPALRAVADAGNDSSNARRRIRLLLAGLPRTTRTLPQP"
BASE COUNT          767 a         1299 c         1425 g          821 t
ORIGIN      
        1 catctggtca cagggattca atccgtgagg caggcgcgat gggcggtcgg cggcgctccg
       61 gatgtaaaac gttgtggggt aaaatgttac ggactcatgt tggccgcgaa ggttggcgca
      121 ttgccccagg acctggcggc gggcccgtga cagcagcggc ggcacttctg gcggctgacc
      181 cggttgtcgc gaacgcaagc cgaaaacaga tcgcttcagc ggtctttccc gaagggcaga
      241 caggccggtc gtttgacgac tggttcacgg tcggccaatt cctccttcac tgtgttaccc
      301 catcaagccc gtcttaaatg aacgggcctg tccttgtcag gtaagcccgt tgaaacgggc
      361 tgagaccata ggaagaagaa ccgcgggcga ccgccaactg gccatatcac ggccggccta
      421 tcagcccgcc tttggcggga agaccccctg aagaggttga tcgcccaacc tggaccggtc
      481 ggcatgcccg cccagactgt tacgcacccc cgggcggcgc cggaagccgc atcgcgttgg
      541 ggcccccagg cctgttgctg tacaataacg ggttcggcag gggtgggcca agggagaaga
      601 tgtatgggca gtccattctg tacgattccc gaagcgatgg acgaactcaa agcaggccgg
      661 tttgtcgtgt tggtcgacga cgagcggcgc gagaatgagg gtgatctgat ctgcgcggcc
      721 gagctgatca cccccgagat ggtcaatttc atgctgaagg aggctcgtgg cgtcctctgt
      781 gtggctctgc ctcgtgacct ctgccgggaa ttggaccttc agccccagac cacggaaaac
      841 acggcgacct tgggcacggc gttcaccgtg accattgacg cccacccgcg attcggtgtc
      901 accaccggtg tctccgccgc tgaccgggca aggaccattc gtcggctgac cgaaccggac
      961 gtgactgagg acgatttctg ccggccgggt cacgttaatc cgctcatggc ccgggaaggg
     1021 ggggtgttag tccgggccgg gcagaccgag ggcaccgtgg accttctgcg cctcgcgggt
     1081 atgtgcccgg tcggagcgtt gatcgaagtc atgaacgacg acggtacgat ggcccgtgtg
     1141 cccgatctgg tgcggttctg tgagcggcac aagctcaaga tgtgcacgat tgccgatctg
     1201 atcgagtacc ggcatcagcg ggagatgctg gttgagcgag tcgagacgat tcatttcccg
     1261 acgccctacg gccggttcac gctccacgcg tacaggtcgc tggtagatat cgagccgcac
     1321 ctggcgctgt gctgcggagg cgtgggggaa cgtgatgcca ccggccgacc cgtgttgcat
     1381 gaggagccgg tgctggtccg cgtccactcg gaatgcctga cgggcgatgt ctttcattcc
     1441 tggcgatgcg attgcggcga tcaactccac cagtccatgc gcatgatcca ggagcaaggc
     1501 aagggggcga tcgtctatct gcgacaggag ggccgcggca tcggcctgca caacaagctc
     1561 aaggcctacc gcctccagga gcagggactg gacacggtcg aggccaacga gcacttgggc
     1621 ttcccggccg acaaacgaga ctacggcatc ggggcccaga ttctccgcga cctgggcctc
     1681 cggcggctgc gaatcatgac caacaacccc aagaagatca gccgcctgga gctttacggc
     1741 atcgaaatcg tcgaacaggt gcccattatc attccgccta acgaggtcaa ccgggcttac
     1801 ctgcggacca agaaggagaa gatggggcac ctgttggagt aggggattgg tctgttggtt
     1861 cgttggtctg ttagtcggtt ggtcccttgg aacaaatgtc gcctgcttgc ctgccgttgc
     1921 cgctcttttc gcaagagcgt taccaagtgc gcagacgcag actcctgaat cggtagtgtt
     1981 gagttacgtt ctctgcacgc cgccggaggg cagcagctac gagaactcga tctacgcgac
     2041 ggccgatgtg cagagcttca agtgcgaggt caccttcacg gccgacacgt tcgcccgctc
     2101 ctttgtccgt gtcgatgacg tattctatgt cggactgggg tgtgaacgtc ccggtgagaa
     2161 cgggccgaag ccctcaactt ccaccggcga tatcctgcgg gtggtgttga acaggtggag
     2221 ttccgggttg cagtcagacg acggcgagtc catactgatc gtagagtttt ggtgaccgag
     2281 agtgtgtgcg aaaaccgacg gggccggtcg ttggggcagt gtcgagccta cctcgctctg
     2341 gtccgctgtg acgaccgaca cctgccatat tccgtcgcca ggaccggcag gtctccgcca
     2401 ccttcggtgg ctccgaccat gccctacggt cttcacacgc attctgagaa ctgatcgcgg
     2461 agagctcgct agctgaatgc tcactcgtga aatcgccaga atcctggacg ctaacctcaa
     2521 ccggacccgc gaggcgctgc gggtgatgga agactacgtt cgcttcgtgc tggacgatcc
     2581 ggcaggctgt gcggtgctca agcagttgcg tcacgatctt cgtgaatgcg tcaggcacgt
     2641 ttctctggaa gacctgctgg gtgcgcgaga cacgccggga gacgtcggga ccggcatctc
     2701 cacggagtct gaacggcatc ggacggatgc ccgcgatgcc tgcgtcgcct cggctaagcg
     2761 gttgtccgag gccctgcgga ccattgagga gtatgccaag acgtttgacg tcggcctgtc
     2821 ggcggcgatg gaatcgctgc gctaccgtgc gtacgaagtc gagcagcggg tgatcatgcg
     2881 cgggccacgt tccgcacggt tcgccagggt gcggctctac gtgctggtga ccgcgtcgat
     2941 gtgccgtcgc gattggctgg aaacggccgc cgaggcgatt gccggcgggg ccgattgcat
     3001 ccagcttcgc gagaagggca tggacgacgg cgagttgctg gtccgggccc gccggttgtc
     3061 ggctctttgc cgcgaacggg cagtggtatt cattgtcaat gaccggccgg acatcgcggt
     3121 cctcagcgac gccgacggcg tccaccttgg ccagacggac atgcctgttg ccgacgctcg
     3181 gcgtatcctg gggcctgatc ggctcatcgg tctgagcacg cacacgcccg aacaattcag
     3241 ggcggcgctt cgcctgactc cggactacat tgccgtcggg ccgatgttcg cctccgccac
     3301 caaaccgcag gagcacgtgc ccggcccctc cctgctggcc ttggccgaat ccgagacgtc
     3361 aattccggtc gtacccgtag gtggcatcac cgtcgataac atgggtatac tgaggcaagc
     3421 cggggccaga cggttgtgcg tatgttccac cgtcatcagt tcggacgatc cgcggacggt
     3481 cgcccgcgcg cttctcggct gagcaggggg tctcattggg ctttacctgg gacgaacttg
     3541 accagctcgc gccaaaggat aaatggcgac ttcccctgcc gccgacgtgc gccaaatgcc
     3601 agtacgatct gaccggcctg cccgaggagc gatgtccgga atgcggtacg ccctttcggt
     3661 gggaggaggt gcgcagacgc accaggcgca tctggaacgt cgcgctccgg ctccgtcatg
     3721 ccaaccagga tgccaagctc ggaatcacca tcggcttaat cgtgtggtgc gcgcttgggt
     3781 tcgttcggct ggtcggcctg gatggcctcg cgccgctggt ctccatcatc gcattcggcg
     3841 gcggggtgat cagcatcgtc ctcggctcgc aggtgctcaa catccggcgc atgcccaagt
     3901 gggcccgtcc gtacgtgggc catcccccgc ccagcatgct tctgggggcc gggtctatgt
     3961 ttctgggcgt atcccagatg gtcggcgcgg ttgttttctg accgcggttc tgcaggtgcg
     4021 tccgagtcac ggttgcggca gggtccgcgt tgtgcggggc agcccggcga ggagcaggcg
     4081 aatccggcgg cgtgcatttg agctgtcatt tccggcatcc gcaaccgctc gcagcgccgg
     4141 cgggtatgag ttgacaggca acacgtccgg ccgatgtccc gagcgtggcc aggcaaagcc
     4201 gaccgggatg acgcgccgga gcaaccggac gaccgcggat gaccatgcag gatcgaggtt
     4261 tcgcggcggc cgtggttgca gatcattcgt cagaaacgaa aaacgcgaga cg
//
LOCUS       DAOTPW010000358         4266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16797_length_4266_cov_5.694134, whole genome shotgun sequence.
ACCESSION   DAOTPW010000358 DAOTPW010000000
VERSION     DAOTPW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16797_length_4266_cov_5.694134"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="P5316_20775"
     CDS             complement(<1..589)
                     /locus_tag="P5316_20775"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRR87448.1"
                     /translation="MKWTVLTSVLLPVALSACDSRPAPPASAPAGDPASQAASAPAPA
                     GAWGGSESCKRCHEEAYAEWKTSHHANALRDIVPDLDHEAFSPRREIRHGTQTSYADM
                     RDGRLVLTTMGPDGKQQDFEPIGVIGVDPLWQYLIPFPGDRLQVTELAFDPAKKEWFD
                     VYGAEDRQYWEWGHWTQRGMNWNDMCAVCHTTSFQK"
     gene            complement(805..3939)
                     /locus_tag="P5316_20780"
     CDS             complement(805..3939)
                     /locus_tag="P5316_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87449.1"
                     /translation="MPPIPQPVFCPRILSMLVLAVVVTSRQVSAAPPEVLRRWDFDRP
                     ADIEGWRKGNGAQDLVVADGSLRLTTSHPDAYVFAPPIEVPLDGLVVRVGLRGEKSGE
                     SAVYWTTKDDPTWSEKQRAPQHVADCESLTQPRGFVDVEFTLGKPADAGRVVTAFRID
                     PLNGPSANKIEIDYVELIRKPPVFDTRLAVKAHHLAVGEQTQATVTIRQLDGRGATGA
                     FEIEIPGISAAQPASQDVNVIHPASPGDAAHSRAIVRFEKPGVHWCRARVSSEEQVLA
                     DSEAAVIVGKGESMPTLPGLRSDRVRLDLVPTPDNKHVGAARWMVAGRLAGWLLPLAE
                     ITVLSGDVAVTRCPVLAVARSSPEMVLLNGQISDLPAWEIEILLSLRRFGEHEAIEIA
                     GRLRGPKGGQVLDFSVPVLRTDRDPAGDPIDRYAIFGGLEFLEPGWSSSSDRAVGDRF
                     ADRWSPHPFKITLPMMAVEAGRMTSALVWQPAVSGSEAGPCATFASPNFLDGQSNHLM
                     KLSVPAREENESSARRPYALTNDRLLSIQAVLVAEPDVPVAMAARWWYDVFGAPTPPP
                     RRYDDRQIYDLIARSLGQTIYWPQENGWRHHWYMGKSSHYNPEIAATLIGHYIETGQT
                     QWVDHTGLKGKQIIDTAVPLSARLVHEAPSRNAMAAMRPDGTWPFTNTDEIRKTCREL
                     TQGKYDSLGEDGSTCVGTCVQQALPILKYAHLTGSGPQIDAALKALDAMRRFRVPRGA
                     QVWEVHQQIPDIRAAALATEAYRMAYEITGNPQWLDDASYWAWCGVPFVYSWRVPIEH
                     RGGFLVATRDKDDWNRHSIPLAEAYHSGRPQVTPYGTVPVLGLTFYVVNWFGVLVQWC
                     GLEWAQHVIELDACRPDPLLRYIADGVVLSGLQQMMDKEPWVGLYPDSWHTQTNIAYP
                     ALIYPGLLMRCLQAQGRLPNWCEPWTRVVRDKLGHRRWHVSSWGKPVEVAPPVAGGPW
                     HVKVAFPAGQDNELLVTNVDKPLAVRVGQDVLDSLAPNAAPTALGWRYNAQRRAMLAR
                     FRQTLPTSEIRVTW"
     gene            4041..>4266
                     /locus_tag="P5316_20785"
     CDS             4041..>4266
                     /locus_tag="P5316_20785"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRR87450.1"
                     /translation="MTDMMRALVFEGPNRMNLRQIPRPRPGPGEVLIRVHATAICGTD
                     IRIFTGRKTREIRHGHPIGHECAGTVAAVGI"
BASE COUNT          762 a         1293 c         1438 g          773 t
ORIGIN      
        1 ccttttggaa actcgtggtg tgacagacgg cgcacatgtc gttccagttc atgccgcgct
       61 gcgtccagtg gccccattcc cagtactggc ggtcttcggc gccgtagacg tcgaaccact
      121 cttttttcgc ggggtcgaag gccagttcgg tgacctgcag acgatcgccc gggaacggga
      181 tcaggtattg ccacaacgga tcgacgccga tgacgccgat aggttcaaag tcttgttgct
      241 tgccgtcggg ccccatggtg gtcagaacca ggcggccgtc gcgcatgtcg gcatatgagg
      301 tctgagtgcc gtggcggatc tcgcggcgag gcgaaaaggc ctcatggtcc agatcgggca
      361 caatgtcgcg cagggcgttg gcgtgatgcg atgtcttcca ctcggcgtag gcctcttcgt
      421 ggcaacgttt gcatgactcc gagccgcccc aggcgccggc gggggccggt gccgaggctg
      481 cctggctcgc gggatcgccg gccggagcag acgccggcgg cgccgggcgc gaatcacagg
      541 ccgacaatgc gaccggcagc aacaccgatg tcaacacagt ccacttcaaa gtcggtcaat
      601 cccgtgcgtc gatcaggggc gtccgttcca tgaacctggg ccatggggtt acgccaaata
      661 ccacatggtc gcccgccggt ggtggcacag cctgcctgcg gcgacgtgac gccacaggtt
      721 ctgccggatc aaagcctact cgacgagcag taagaacaaa agcgcccgca catctctgcg
      781 ggtgcaagcg agcgattgat ccggttacca ggtaacccga atctccgatg tcggcagggt
      841 ttgcctgaat cgcgcaagca tggctcgccg ttgggcgttg tagcgccagc caagagccgt
      901 tggcgcggcg ttgggggcca gggagtcaag gacatcctgg ccgacgcgga ccgcgagcgg
      961 cttgtcgacg ttggtcacga gcagttcgtt gtcttggccg gccgggaagg cgactttcac
     1021 gtgccatggt ccgcccgcga ccgggggagc gacctcaacg ggcttgcccc aggaactgac
     1081 atgccagcga cggtggccga gtttatcgcg gacgacgcgc gtccagggtt cgcaccagtt
     1141 gggcagccgg ccttgtgcct ggaggcaacg catcagcaga cccggataga tcagggcggg
     1201 gtaagcgatg ttggtctggg tatgccagga gtcggggtag aggccgaccc acggctcctt
     1261 gtccatcatc tgctgcaagc cactgaggac cacgccgtcg gcgatgtacc gcagcagcgg
     1321 gtcgggacga caggcgtcca gttcgatgac atgctgagcc cattcaaggc cgcaccactg
     1381 cacgagtacg ccgaaccagt tgaccacgta gaacgtcagg ccgaggactg ggacggtacc
     1441 gtagggcgtg acctggggcc ggccgctgtg gtaggcttcg gccagcggaa tggagtgccg
     1501 gttccagtcg tccttgtcgc gagtcgccac caggaacccc ccgcggtgtt ctatgggcac
     1561 gcgccaactg tagacgaatg gcacgccgca ccaagcccaa taggaggcat catccagcca
     1621 ttgcggattg ccggtaatct cgtaagccat ccggtaggcc tcggtcgcca gggcggcggc
     1681 ccggatgtcg ggaatctgct gatgcacctc ccagacttgg gcgccgcgag gtacgcggaa
     1741 gcgccgcatg gcgtcgagcg ctttaagggc ggcatcgatc tgaggcccgg atcccgtgag
     1801 atgagcgtac ttgaggatcg gtagagcctg ctggacacag gtgccgacgc aggttgagcc
     1861 gtcttcgccg aggctgtcgt acttgccttg cgtcagctca cggcaggtct tgcgtatttc
     1921 gtcggtgttg gtgaaaggcc aagtgccgtc tggtcgcatg gcggccatcg cgtttctcga
     1981 cggtgcttcg tggaccaggc gcgcggacag cgggaccgcc gtatcgatga tctgcttgcc
     2041 tttcagcccg gtgtgatcca cccactgcgt ctgtccggtc tcgatgtagt ggccgatgag
     2101 cgtggccgcg atttccggat tgtagtggct gcttttgccc atgtaccagt gatgccgcca
     2161 accgttctct tgaggccagt aaatcgtctg gccgaggctg cgggcgatca ggtcgtatat
     2221 ctggcgatcg tcgtaacgac gaggcggtgg cgtgggcgcg ccgaatacgt cgtaccacca
     2281 gcgggcggcc atagcgacgg gaacgtcggg ctcggcgacg agcactgcct gaatactcag
     2341 cagtctgtcg ttcgtgagtg cgtacggccg tcgggctgac gactcatttt cctcacgagc
     2401 aggcacggag agcttcatca ggtggttgga ttgtccgtcg aggaagttgg gcgatgcgaa
     2461 agtggcacag ggaccggcct cagagcctga aaccgccggc tgccatacaa gtgccgaggt
     2521 catccggccg gcttcgacgg ccatcatggg cagcgtgatc ttgaagggat ggggcgacca
     2581 gcggtcggcg aacctgtctc ccacggcacg atcgctcgat gatgaccagc cgggctccag
     2641 gaattccaga ccgccaaaaa tggcgtaacg gtcaatcggg tcgccggcgg ggtcgcggtc
     2701 tgtccgaagc acggggaccg aaaagtcaag cacttgcccc ccttttgggc ctcgcaggcg
     2761 accggcaatc tcgatcgcct cgtgctcgcc gaatcttctc aagctcagca gaatctcgat
     2821 ttcccaagcc ggcaggtcgg agatttgtcc gttgagcaat accatttcag gtgacgatcg
     2881 ggccaccgcc aagaccgggc agcgcgtcac ggccacgtcg ccgctgagca ccgtgatctc
     2941 ggccagcggc agcagccagc cggcaagtcg gccggcgacc atccagcggg ccgccccgac
     3001 atgcttgttg tcaggggtgg gaacaaggtc gagccggacg cgatcggacc gcaaacccgg
     3061 cagcgtcggc atcgactcgc ctttgccgac aatgaccgcg gcctcggagt cggcgagcac
     3121 ttgctcctcg gacgagacac gagcccggca ccaatgcacg cccggctttt cgaaacgcac
     3181 gatggccctg gaatgggcag cgtcgcccgg tgaggcgggg tgaatgacat tgacgtcctg
     3241 cgatgccggc tgagcggccg agatgccggg gatttcgatt tcaaaagcgc cggtcgcgcc
     3301 gcggccgtcg agttggcgga tcgtgaccgt cgcctgtgtc tgttctccga cggcgagatg
     3361 atgggctttg actgcaagtc gcgtgtcgaa gacgggtggc ttgcgaatca gttcgacata
     3421 gtcaatctca atcttgtttg cggaggggcc gttgagcggg tcgatccgaa atgcggtcac
     3481 gacacggccg gcgtcggcgg gcttgcccag cgtgaactca acgtccacga agccgcgggg
     3541 ctgggtcaat gattcgcaat cggcgacgtg ttgtggcgca cgctgctttt cgctccaggt
     3601 tggatcatct ttggtggtcc agtaaacggc tgattcgccg ctcttctcgc cgcgcaggcc
     3661 gacacgcacg acgagaccgt ccaagggcac ctcgatcggg ggcgcgaaga cataagcatc
     3721 ggggtgagat gtggtaaggc gcaggctgcc atcggcgacg accaggtcct gcgcaccgtt
     3781 tcctttccgc cagccttcga tgtcggcggg tctgtcgaag tcccagcggc gcaggacttc
     3841 cggcggggcg gccgacactt gccggctggt gaccacaacg gcgaggacca gcatggaaag
     3901 gattcgagga caaaaaaccg gttgaggaat cggtggcatg ggctgtttcc ttctgttgca
     3961 gggtcttcga gcggccgact gcccgctgcc cgcttcgcac cctgcacatg agatgctacc
     4021 atatccggcc ggaggcgacg atgaccgaca tgatgcgggc actggtgttt gaaggcccca
     4081 atcggatgaa cctgcgtcag atcccgcggc cccggcccgg gccgggcgaa gtgctgattc
     4141 gcgtccatgc gacggccatc tgcggtacgg acatccgcat cttcaccggt cgcaaaacgc
     4201 gagagatccg gcatggtcat cccatcgggc atgagtgcgc cgggacggtc gccgccgtcg
     4261 gcatcg
//
LOCUS       DAOTPW010000359         4261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16823_length_4261_cov_5.627199, whole genome shotgun sequence.
ACCESSION   DAOTPW010000359 DAOTPW010000000
VERSION     DAOTPW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16823_length_4261_cov_5.627199"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            583..2406
                     /locus_tag="P5316_20790"
     CDS             583..2406
                     /locus_tag="P5316_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRR87451.1"
                     /translation="MSNIPLEQGVREICQACGNDRTRLMDICRAVQNQFGCVSGEAMD
                     LIARECRCPRVEVESVVSFYAFFSDKPKGKIVIRLCDDIVDRLHGYDRVAKALSQELG
                     IALGQTTSCGSFTLERTPCIGMCDQAPAAMVNDVIVTKLNGASAREMIKELREHMDPR
                     KLVTRLGDGHNADDRIHAMVHNNIRKKGPVIFAPYETGTGLKKALAMTPAEVIRDVKT
                     ARLRGRGGAGFPTGIKWEFTRASQGDRKFVICNGDEGEPGTFKDRVLFTECADMLFEG
                     MTIGGYAIGADTGILYLRGEYAYLKTYLDSVLDERRRKNLLGKNILGKNDFSFDIRIQ
                     MGAGAYICGEETALISSCEGRRGDPKNRPPFPAQKGYLDYPTVVNNVETFCCVSRILD
                     KGAGWFAEMGSKGSPGTKLLSISGDCKAPGVFEVPFGTCLKDVLEMAKAEDPLAAQVG
                     GPSGQLVPAKDFGRTICYDDLATGGAIVVFNKARDLLEVVDYYVEFFLEEGCGYCTPC
                     RVGNVLLKERIGKLLRGKGEPGDLEYLKELGETMKTMSRCGLGQTAGNPVLTSLANFP
                     ELYQAKIRKHETVFQPTFDIKAAVADAERLVGRKSVIFAGK"
     gene            2424..3137
                     /locus_tag="P5316_20795"
     CDS             2424..3137
                     /locus_tag="P5316_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRR87452.1"
                     /translation="MSNTFTFTIDGVEVQASPGQTILEAADAAGIYIPRLCHMKGLIP
                     YGSCRVCTVMVNGRPQASCTQPAAPGAVVLNDTEALRTFRRNIIDMLFVEGNHFCMFC
                     EKSGNCELQALAYRFGITAPKYPYQNPKRDVDASHPDIFIDRNRCILCARCVRASRDL
                     DGKNVFQFVGRGSRKKIGVNASARLKDTGMDVTDKAADVCPVGAILKKRAGYAVPIGK
                     RLYDQKPIGSDIEAARAGK"
     gene            3150..3695
                     /locus_tag="P5316_20800"
     CDS             3150..3695
                     /locus_tag="P5316_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HRR87453.1"
                     /translation="MAKPRIATTSLAGCFGCHMAILDIDDRILKLVELVDFDRSPVDD
                     FKHITGRCAIGLIEGGCCNEENVHVLQEFRENCDILISLGDCAIMGGIPAMRNTIPLQ
                     ECLEEAYLKCPTCYNPSGKIPNDPEIPLLLNKVYPCHEVVKIDYHLPGCPPSADTIWE
                     ALVALLNNKPMELPYQLIKYD"
     gene            3750..>4261
                     /locus_tag="P5316_20805"
     CDS             3750..>4261
                     /locus_tag="P5316_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRR87454.1"
                     /translation="MPVTRVEGHGKVTILLDSKNKVKQARLHIVEFRGFERFIQGRPY
                     WEVPVLVQRLCGICPVSHHLAAAKAMDRIVGADKLTPTAEKMRRLMHYGQFFQSHALH
                     FFHLCSPDLLFGFDADPAVRNVIGVAAKFPDLAVQGVMMRKYGQEIILRTAGKKIHGT
                     GAIPGGINKNL"
BASE COUNT          925 a         1386 c         1189 g          761 t
ORIGIN      
        1 tcatcgatga aaccaaatca gggggtaaaa caatgacgaa atgccaaact ccaggcatgc
       61 ccacgcaaag ccgtgggcat ggcacccggc gatcccgaaa catgcgcgca agccgcacag
      121 atatctggcc gtagggttct aattgaaccg ccaaggcccc cgccatcagc tcttggacca
      181 caacaacgcc gttttcgggt gggacgaggc cttgcaattc cgcgcgaact tggctatgct
      241 ctggctcgtg atttttttca cgaatccgga cagaacggtc cgctgtggct acctctgtcc
      301 gcacgcccag gtcacgccgt cagtcgaact tacggccatc cggagcaagg gcaaacccgc
      361 agacccgacc tccagaaaac caggggcgaa agacgatggg tttgggtaac cttcgtggag
      421 aagagagctc cctggttacg gcaccccatg ggtcatggtg ttgaaagagc accgtgtttg
      481 tcggactatt tggagcgttc tcaagccttg cactcgcgcc ggcctgatgc cggcaacgca
      541 aggtttgtca gtgagcactc ccagtcagga tcgaggtaat gcatgagtaa catccctctc
      601 gaacagggcg tccgggagat ttgtcaggca tgcggtaacg accgcacgcg cctgatggat
      661 atctgccggg cagtccaaaa ccagttcgga tgcgtctccg gcgaagcgat ggatcttatc
      721 gccagggaat gtcgttgccc gcgcgtcgaa gtcgaaagcg tcgtctcttt ctacgctttc
      781 ttctccgaca agcccaaggg caaaatcgtc atccgcttgt gcgacgacat cgtggaccgg
      841 ctgcacggtt atgaccgcgt cgccaaggcc ttgtcacagg aactgggtat cgccttgggc
      901 caaaccactt cctgcggcag tttcacgctg gaacgtacgc cctgcatcgg catgtgcgac
      961 caggccccgg ccgcgatggt caatgacgtc atcgtcacca agctcaacgg cgccagcgcc
     1021 cgtgaaatga tcaaagagct gcgcgagcac atggaccctc gcaaactggt gaccagactc
     1081 ggcgacggac acaacgccga cgatcgcatc cacgccatgg tccataataa cattcgcaag
     1141 aagggcccgg tcatctttgc tccctacgaa accggtacgg gtctcaagaa agccttggcc
     1201 atgacgccgg ccgaggtgat tcgcgacgtg aagaccgccc ggctccgcgg acgcggtggc
     1261 gcaggctttc ccaccggaat aaaatgggag ttcacccgcg cctcgcaggg cgatcgcaag
     1321 tttgtcatct gcaacggcga cgaaggcgaa cccggcacct tcaaggatcg cgtgctcttc
     1381 accgagtgcg cggacatgct ttttgagggc atgactatcg gcggctatgc catcggcgcc
     1441 gacaccggca tcctctacct ccgcggcgaa tacgcctacc tcaagaccta cctcgacagc
     1501 gtcctcgacg aacgccgcag gaaaaacctg ctgggaaaga acatcctcgg taagaatgac
     1561 ttcagcttcg atatccgcat tcaaatgggc gccggggcct acatctgcgg cgaggaaacc
     1621 gccctcatca gctcctgtga gggccgaagg ggcgatccga agaaccggcc gccgtttccc
     1681 gctcagaagg gctatctcga ctatcccacg gtggtcaaca acgtcgagac cttctgctgc
     1741 gtctcacgga ttctcgataa gggcgccggt tggttcgccg agatgggctc caagggcagc
     1801 ccgggcacca agcttctgag catctccggc gactgcaagg cccccggcgt tttcgaagtc
     1861 ccctttggca cctgtctcaa agacgtgctc gaaatggcca aggccgaaga ccccctcgcc
     1921 gcccaggtcg gcggccccag cgggcagctt gtcccagcca aggacttcgg ccgtaccatc
     1981 tgctatgatg atctcgccac cggcggcgcc atcgtcgtct tcaacaaggc ccgtgatctg
     2041 cttgaggtcg tcgactatta cgtcgagttc ttcctcgaag aaggctgcgg ctactgcacc
     2101 ccctgccgcg tcggcaacgt cctgctcaag gagcgcatcg ggaaactcct ccgtggcaag
     2161 ggcgagcccg gcgacctgga atacctgaag gaactgggcg agaccatgaa gaccatgagc
     2221 cgttgcggcc tcggccagac cgccggcaac cccgtcctga cgagcctggc caacttcccc
     2281 gaactctatc aggcgaaaat caggaaacac gagacggtct tccagccgac cttcgacatc
     2341 aaggccgccg tggccgacgc cgaaaggctc gtcggacgca agtcggtgat ttttgcaggc
     2401 aaataggatg ataaggaatt gacatgagca acacatttac gtttacgatc gacggcgtcg
     2461 aggtgcaggc ctcacccggc cagaccattc ttgaagcggc cgatgccgcc ggcatctaca
     2521 tcccccgact gtgccacatg aagggcctca tcccctacgg ttcctgccgc gtgtgcaccg
     2581 tgatggtcaa cggccgtcca caggcttcct gcacgcagcc ggcggcgccg ggggccgtcg
     2641 tactcaacga caccgaagcc ctccggacgt tccgcagaaa catcatcgac atgctgttcg
     2701 tcgaaggcaa ccacttctgc atgttctgcg aaaagagcgg caactgcgag ttgcaggccc
     2761 tcgcctaccg cttcggcatc accgcaccca agtatccgta tcagaacccc aagcgggacg
     2821 tcgacgcatc gcatcccgac atcttcatcg atcgcaaccg gtgcatcttg tgcgcccggt
     2881 gcgttcgcgc gtcccgcgac ctggacggca agaatgtatt tcagttcgtt ggccgcggct
     2941 ccagaaagaa aatcggcgtc aatgcctcgg cccgcctcaa ggacacggga atggacgtta
     3001 ccgacaaggc cgccgacgtc tgcccggtgg gcgccatcct caagaagcga gcgggctacg
     3061 ccgtccccat tggcaagcgg ttgtatgacc agaagcccat tggttccgat attgaggccg
     3121 cacgggccgg caagtgaggt ataggaaaca tggctaaacc aagaatcgca accacgtccc
     3181 tggccggctg cttcggctgc cacatggcca tcctcgacat cgacgaccgc atcctcaaac
     3241 tggtcgagct cgtcgacttc gacaggtcac cggtcgacga cttcaagcac atcaccggac
     3301 gctgcgccat cggcctgatc gaaggcggct gctgcaacga ggaaaacgtc cacgtcctcc
     3361 aggaattccg cgaaaactgt gacatcctta tctctctcgg cgactgcgcc atcatgggcg
     3421 gcatcccggc tatgagaaac accattcccc tgcaggaatg tctcgaagag gcatacctga
     3481 agtgcccgac ttgctataac ccctcgggga aaatacccaa cgaccccgag atcccgctgc
     3541 ttctcaacaa ggtctacccg tgccacgagg tggttaagat cgactaccac ctgccgggct
     3601 gcccgccctc ggccgacacc atctgggaag ccctggtcgc gctgctgaac aacaaaccga
     3661 tggaattgcc ttaccagctc atcaaatacg attgagatcg gaggaacacc gtgtctgcat
     3721 cacccaaaag tggcaacaaa atcaccatca tgccggtcac tcgcgtcgag ggccacggca
     3781 aggtgacgat cctgctggac agtaagaaca aggtcaagca ggcccggttg cacatcgtcg
     3841 agttccgcgg gttcgagcga ttcatccagg gccggcccta ctgggaagtg cccgtcctgg
     3901 ttcagcgatt gtgcggcatc tgcccggtca gccaccatct ggcagccgcc aaggccatgg
     3961 accgcatcgt cggcgctgac aagctcaccc cgacagccga aaagatgcgc cgactgatgc
     4021 actacggaca gttcttccag tcccacgcct tgcacttctt ccatctgtgc tcgcccgacc
     4081 tgctgttcgg ctttgacgcc gacccggccg ttcgcaacgt gatcggcgtg gccgccaagt
     4141 tccccgatct ggccgtccag ggcgtcatga tgcgaaagta cggccaggag atcatcctgc
     4201 gaaccgccgg caagaagatc cacggcaccg gcgccatccc cggcggcatc aacaagaacc
     4261 t
//
LOCUS       DAOTPW010000360         4251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16877_length_4251_cov_4.823165, whole genome shotgun sequence.
ACCESSION   DAOTPW010000360 DAOTPW010000000
VERSION     DAOTPW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16877_length_4251_cov_4.823165"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..989)
                     /locus_tag="P5316_20810"
     CDS             complement(75..989)
                     /locus_tag="P5316_20810"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HRR87455.1"
                     /translation="MLNALTVDLEDWPQAVLDPSLPITDRVVGNVDRLLGLLDRHSVR
                     ATFFALGKVCERHPQLLPLIQSAGHEIASHGYGHELMYRLTPRQFEEDVRRSIEIIGS
                     QTGRRPLGYRAPAFSITRESLWGGPILASLGFRYSSSIFPIRKARYGIHDAPRIPHRW
                     SNCDLVEFPLTTLRVFGCHVPVCGGGYMRLLPWWVHAHAIGSMNAGGHPAVVYLHPYE
                     LASDEVDLFRRAGLRFSTSRRVMQSLWRSRVLPRLTRLFEAFRFAPITQVFEAINLPG
                     LCPAAPGTVPELSPPCLSLVPPPTCTAT"
     gene            1387..1470
                     /locus_tag="P5316_20815"
     tRNA            1387..1470
                     /locus_tag="P5316_20815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1421..1423,aa:Leu,seq:cag)
     gene            1896..3941
                     /locus_tag="P5316_20820"
     CDS             1896..3941
                     /locus_tag="P5316_20820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRR87456.1"
                     /translation="MGTAVLENATKGTMGAPSGSPLLSGGLAGLLRREWLITNGRGGY
                     ASGTVAGVPTRRYHGLLVAAARPPLERWLFLSGVLERVGINGRFAEAATFEFDKTFHP
                     EGYRLLTDFAVSNNAEMPWVRFTYAIEGVKMTKRIVLLRDRDEVCIRYRIEAPPGTVL
                     SLGLLPFLAMRDFHALTRAFEGSYPLTLADNLVAVDAFTSGPRVWLTAGSPGQDQGVA
                     FESQPHWWRQFEHREEAARGLDHSEDLFVPGWFKAAGMGSLEVEFRAVADFTPDFSGR
                     PEPRPFASDNVTESGRALPVEERLREAAGAFVVSRKRPEGSSLTTILAGYHWFGDWGR
                     DTFIALPGLLIETGRLGEARQVLEVFASAQQDGLIPNRFSDYGDGRDYNSVDASLWFI
                     HAADAYTAASGDDGCWREKLGPACERAVEAFLAGTKFNIHVDGDGLVACGDASTQLTW
                     MDAKCGDTVFTPRHGKPVEINALWYNALRLLSRRVSAYEPGRASRYADLAGKVEQAFR
                     QVFWNEKCRCLYDVVRDGWADPAVRPNQILAVSLPYSMLDRERQRAVLAIVERQLLTP
                     YGLRSLCPRHPSYAGRYEGNPYRRDSVYHQGTVWAWLMGPYVEAYLRVHDFSPEAKRR
                     MRQLIQPLVDHLDDAGIGSVSEIFDGDPPHTPRGCIAQAWSVGELLRAWHMTEPVGG"
BASE COUNT          768 a         1206 c         1438 g          839 t
ORIGIN      
        1 ggggatcgga cgctgcctgc caatcggatg gcagcatacg gttttcggag gcctcgttct
       61 tgcgcaggta gtcctcacgt cgcggtgcac gtcggtgggg ggacgagtga caggcacggg
      121 ggcgataatt cggggaccgt gcccggagcg gcagggcaaa ggccgggcag attaatggct
      181 tcgaatacct gcgttatcgg agcaaagcgg aaggcctcga aaaggcgggt caggcgaggc
      241 aacacgcgag atcgccacaa cgactgcatg acgcggcggc tggtggagaa gcgcaatccg
      301 gccctgcgaa ataaatcgac ctcgtccgaa gcaagttcat agggatgcag atagacgacg
      361 gccgggtggc cgccggcgtt catgctgccg atggcgtgcg catgaaccca ccagggcagc
      421 agtcgcatat aaccgccgcc gcagacgggc acatggcagc cgaaaacgcg cagcgtggtc
      481 aatggaaact caacaagatc acaattgctc cagcgatgcg ggatgcgcgg cgcgtcgtga
      541 ataccatagc gagccttccg gatcggaaag atgctcgaac tgtaccgaaa accgaggctt
      601 gccagaatcg gccctcccca gagcgattct cgggtgatcg aaaacgccgg ggcccggtaa
      661 cccaacggcc gccgtccggt ctgcgatccg atgatctcga tcgatcgcct gacgtcctcc
      721 tcaaattgcc gaggcgtcag ccgatacatc agttcgtgcc cataaccgtg cgaggcaatc
      781 tcatgcccgg ccgactgaat caggggcaga agctgcggat gccgctcgca cacctttccg
      841 agggcaaaga aggtggcccg gactgaatgc ctgtccagca gcccaagcag acggtcgacg
      901 ttgccgacga cccggtcggt aatcggcagg ctcggatcga ggacggcctg aggccagtcc
      961 tcgaggtcga cggtcagggc gttgagcatg cgagcttcct ggggcctttc ttctattgga
     1021 tcaatcggaa ggtttgaccc gcggctttgg ttctcggacg ccaacgcgga ccgccttttg
     1081 gcgggtcgca gcccgaacac accgcaatcc accaacatcc gcgtacaatg gacagatctc
     1141 tgtccgtaaa gtcctgaggc ggaccgcctt gctgcgggcc gcagcccaaa cacaccgcta
     1201 tccacgaaca tgcgcgtaaa acggacagat ctctggccat aaggccataa aaatctgcca
     1261 gcaccctttc gaaaggcttt cccgccgctg cctggaatcc tgacagatcg gtgggaagcc
     1321 aggccctcca aggccggttt attgacaagg accggcctcc ggcgttaaat ggactttgag
     1381 atcgccgcgg atgtggcgga attggcagac gcgcaaggtt caggtccttg tgggggcaac
     1441 cccgtggagg ttcgactcct ctcatccgca attcctcgat cattagcctc ttccatcggg
     1501 actggtgtgc actttgcgcg catgtttttg gatcgcagat cgcgtgggct gcggtccgcc
     1561 ttagacggaa tgtcgtctca tttttatgtg aggtcacctg ttgacgactt ccgtagcatt
     1621 cgcctatcgg tcaccgcttc aaattggccg ctcagaggta atgattgtgg gatatgtttt
     1681 acagctttgc ggttattgaa tggcgccgcc ctcgtactcc tgtcaccatc cgcaaggatg
     1741 cgatcacggc gtctcgaggc cttcttttcc cgtggcgtcc ggactgtgtg gcgagttgcc
     1801 agcgacaaat ggcgtaagga tcggtctcgt gccgatgatt aggcgggggg gttatggtta
     1861 taatcagtga atctcactgg gaggtcggag gctcgatggg aacggcagtc ctggagaatg
     1921 caacgaaggg cacgatgggt gcgccgagtg gatcaccgct gctctcaggg ggccttgcag
     1981 ggctgctgag gcgggagtgg ctcattacca acgggcgggg ggggtatgcg tccgggaccg
     2041 tggcgggggt gccgacgcgt cgctatcacg gccttctcgt agccgccgct cgcccccctc
     2101 tggaacgctg gctgtttctg agcggggtgc ttgagcgcgt ggggatcaac gggcggttcg
     2161 cagaggcggc aactttcgag tttgacaaga cgttccatcc ggagggttat cggctactga
     2221 cggatttcgc ggtcagcaac aatgccgaga tgccttgggt gcgtttcacc tacgccatcg
     2281 aaggcgtgaa gatgaccaag cgcatcgtac tgctgagaga ccgggatgag gtctgtattc
     2341 gctatcgtat cgaggcccca cccggcacgg ttctctcatt ggggctcttg ccgttcctgg
     2401 cgatgcgtga tttccacgcg ttgacccgcg cgtttgaggg cagctatccg cttactctgg
     2461 cagacaactt ggttgcagtt gatgccttca cgtcaggccc gcgcgtgtgg ttgacggccg
     2521 gttcgccagg ccaggatcag ggtgtggcgt tcgaaagcca gccgcactgg tggcgacagt
     2581 ttgagcatcg ggaggaggcg gcacgagggc tggatcacag cgaggacctg tttgtgccgg
     2641 gttggttcaa agcggcgggg atgggcagcc ttgaggtgga gtttcgcgcc gttgccgatt
     2701 tcaccccgga tttctcaggc cggccggagc cgcggccgtt tgcctccgac aacgtgacgg
     2761 agtccggcag ggcgctgccg gtggaggagc gtctcagaga ggccgcaggt gcgtttgtgg
     2821 tcagccgcaa gcgaccggaa gggtcgagtc tgaccacgat cctggcaggc tatcactggt
     2881 tcggcgactg ggggcgcgac acgttcatcg cccttcccgg cctgctgatc gagaccgggc
     2941 ggctgggcga ggcgagacag gttttagagg tttttgcctc cgcacagcag gacgggctga
     3001 tcccgaaccg tttcagcgat tacggcgacg gtcgggacta caacagcgtc gacgcgagcc
     3061 tgtggttcat ccacgcggcc gatgcctaca cggcggccag cggcgatgac ggctgctggc
     3121 gcgagaagct cgggccggcg tgcgagcggg cggtggaggc atttctggcg ggcacgaagt
     3181 tcaacattca cgttgacggc gacgggctgg ttgcctgcgg ggacgcctcg acgcagttga
     3241 cctggatgga tgccaaatgc ggcgacacgg tattcacgcc gcgtcacggc aagccggtcg
     3301 agatcaacgc cttgtggtac aacgccttgc ggcttctgtc gcggcgtgtt tcggcttacg
     3361 agcccggccg tgcgagccgt tatgcagatc tggcgggcaa agtggaacag gcgttcaggc
     3421 aggtattctg gaatgagaag tgccggtgct tgtacgacgt ggtgcgtgac ggctgggccg
     3481 atcctgcggt gcgaccgaac cagattctgg ccgtcagcct gccgtattcg atgctcgacc
     3541 gcgagcgtca gcgggcggtt ctggcgatcg tcgagcggca actgctgacc ccttacggcc
     3601 tgcggtcgct ctgtccgcgg cacccgtcgt atgccggccg ttacgagggc aacccctatc
     3661 ggcgcgacag tgtgtaccac cagggcacgg tatgggcttg gctgatgggt ccgtatgtgg
     3721 aagcatacct tcgcgttcat gatttctcgc ccgaggccaa gcgccgcatg cgccagctta
     3781 tccagccgtt ggtcgatcac ttggacgacg cgggcattgg ctcggtcagc gaaatcttcg
     3841 acggcgaccc gccgcacact cctcgtggct gcattgccca agcatggagc gtgggtgagc
     3901 tgcttcgggc gtggcacatg accgaaccgg tcggtggttg aatccgcagg cgcatccagg
     3961 gttcggccag ccatgccgtg cgcctcccag gggttgggtg atcgatatcg actgctgcct
     4021 cagggagagg ttattcccga tcataaatag cttcaaataa ggacttatac gtccgatggg
     4081 tttgtttggc gcggggggtg gtcggcggtc ggtgtccggc ttttggcgcg cgagggtcgc
     4141 ttcgggatct gttgcggcct ggccatgtcg ccagccgagg atcagcggtc ggtcagaggg
     4201 gacggagaag ggtttgcgtc cggccgggag gtcatcgctt gcggcgttgg g
//
LOCUS       DAOTPW010000361         4248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16896_length_4248_cov_4.941331, whole genome shotgun sequence.
ACCESSION   DAOTPW010000361 DAOTPW010000000
VERSION     DAOTPW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16896_length_4248_cov_4.941331"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            475..1035
                     /gene="efp"
                     /locus_tag="P5316_20825"
     CDS             475..1035
                     /gene="efp"
                     /locus_tag="P5316_20825"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRR87457.1"
                     /translation="MVKASELKRGQVIDYEGTRYTIKDIQHVAKGNWRSYYQVKLKNF
                     VTGQIIDQRMAVDDRVELVFVETKDFEYLYRNGDEYVLADPETYDQIPVSADLFGDSI
                     RFLKENIRVTCSIIDGQIVNIDLPNTVELAVKDTPPVVKGATATNQPKEAILETGAKI
                     RVPSFIQVGEVVRVDTRTGEYLERAK"
     gene            1389..2033
                     /locus_tag="P5316_20830"
     CDS             1389..2033
                     /locus_tag="P5316_20830"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87458.1"
                     /translation="MTARPLTGRQLNVLRFAVDFLRDRRFPPTLRQITAALGLAGINA
                     VRGHLAALQRKGDIVRDAEKARSIRVVHPPSALGRVKRKLHELFRTDENVFHRIIYGL
                     AWTTKDRCPWLAGPTVESVKAALDRAAVEPGWTILDRPIKPDLVVVVATPAKPFRATD
                     GAEISIDIPQHAPRRAVISGRRRWARDSWPRPIWRCSTCWSTVWSAARGVLHKG"
     gene            2039..2920
                     /locus_tag="P5316_20835"
     CDS             2039..2920
                     /locus_tag="P5316_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87459.1"
                     /translation="MIKTAFALLLGLLPALLIAAGCEKGATSPRGQDSPPANPSTVPA
                     LRDGEQPSDGVPIEELAAAFPSGYELQNKRLSHVGLGYHYLYKAANTDKRLAVTVGLF
                     GNSAKARNAALLSHDLMPVKPRGLDGIADEAWIWDEDDKAAVRFRLGRLLCRVGGDVS
                     VPEARQIAERMVLQLSDRLPLLRRDDGDVSSAAWSGIPAHMRPGESAVVRFNAGTVTA
                     PERMEAGVWASAGRVSAKSEGTFRFTAPVRPGPLQIRCFATSQANRVEVSVFDVQVNI
                     QERKPGNEHKTRTSAGN"
     gene            2886..3068
                     /locus_tag="P5316_20840"
     CDS             2886..3068
                     /locus_tag="P5316_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87460.1"
                     /translation="MNTKHEHLQGTETTVSPDGAGVEGYGGSERPGPDACEATTYERP
                     VLIRRDESREGDFWCD"
     gene            3061..3372
                     /locus_tag="P5316_20845"
     CDS             3061..3372
                     /locus_tag="P5316_20845"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRR87461.1"
                     /translation="MTEAPPGNEKPRFRRERVRLQETTTGLLVYDIESDSLFHANRTA
                     SMVLELLDGTRSWAQIAHALADRHGWATAEIAADLSTFARELMTVGLLEEPPWNSCIH
                     P"
     gene            3348..>4248
                     /locus_tag="P5316_20850"
     CDS             3348..>4248
                     /locus_tag="P5316_20850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR87462.1"
                     /translation="MEFMHPPLRVSIDVTGACNLSCRHCRHGSQACDELTLAEISSVI
                     DDVGQMGVFRLVISGGEPFLRRDILEILLRALRTEVGRVFVSTNGTCMPKRVPDRLTA
                     FRRRLTFKISLDGAPDFHDAWRGRKGAFAKASASIRLLTQRGFDVQVTTTLMPSNLDS
                     LSWLLEYVAETGSSRHALVEVIPVGRAGVEMALSTDERIRAAEAIRFARSRGGHCEII
                     SKIAFVDGRCSGFFCSGGIEECGVLSDGRIVGCRLMPDVVAGNVREMPLSRVWSSSER
                     FSLFRGITPARLQKTCRDCEMAAA"
BASE COUNT          868 a         1322 c         1265 g          793 t
ORIGIN      
        1 tggtagtcag attcagatgg attcggtact cgttcatggc tcctccttga gcatacggga
       61 cacgaaaagg tttcctgaga aacatcctct cacgagtctg attgtctgtc cactcttcaa
      121 cactgaggaa tcgaggcgtg acattcccct cgctggtctc tattccccat cgcgacgcag
      181 acgttccaac aacccacggc cggctatccc caaacagatg accgccttgt cctacatggc
      241 attatcgggc atttcggtcc cggcacttcg accaggctca tcgccactcg gccttggaaa
      301 ctcgaccggg ggatattgac tgccccggcc tgatctggga cactatcaat caaaagcccg
      361 gcaaacacgc cggctcgttc attcccccga ttcccgcgat ggtgagagga tcattggggc
      421 gacaggacca ggaaatggtg ccgcctgcga agcggcgaac acagagacta tcgaatggtt
      481 aaggcaagcg aactgaaacg tggccaggtc atcgattacg aaggcacgcg ctatacgatc
      541 aaggacatcc agcacgtggc caagggcaac tggcgaagct actatcaggt gaaactgaaa
      601 aacttcgtca ccggccagat catcgaccag cgcatggccg tcgacgaccg cgtcgaactg
      661 gtcttcgtcg agaccaagga tttcgagtat ctctacagga acggcgacga atacgtgctg
      721 gccgatcccg aaacctatga ccagataccg gtcagcgccg atctgtttgg cgattcgatc
      781 cgtttcctca aggagaacat ccgagtaacc tgctcgatca tcgacggcca gatcgtcaac
      841 atcgatctgc ccaacaccgt cgaactggcg gtgaaagaca ccccgcccgt ggtcaaagga
      901 gccacggcca ccaatcagcc caaggaggct attctggaga ccggcgcgaa gatcagggtc
      961 ccgtccttca tccaagtcgg cgaggtcgtc cgggtggata cccgcaccgg cgagtacctc
     1021 gaacgggcca agtaaccgcc cccgccggcc ctggaatgta gtcctctctg caccaacaga
     1081 aaaccgtctt gaaagcgggc tccaagctgc atgtcgctgg ccccagaatc ccctggggcc
     1141 tctgtaaccg cccgccggcg caacacgggt ccgtgcaaga ctgaccaacc gaaaacggca
     1201 ccaagccaag gcagtacccc aaaacctgtt tgccggcgcc tcgtgtgccc gcaagcaagc
     1261 cggctgcggc acccacggcg cgccctcgcg ggatgtgtca cgaaatccgt ttgatcgccc
     1321 gccgagccgc ttgacaggct gactgttatg tcgtacactg tacataagta cactatacgg
     1381 ggaccgccgt gaccgccaga ccactcacag gccggcaact caacgtattg cgattcgcgg
     1441 tagactttct ccgcgaccgc aggtttccgc ccacccttcg ccagatcacg gccgcgctgg
     1501 ggctggccgg catcaacgcc gtgcgcggac atctcgccgc ccttcagcgc aaaggtgaca
     1561 tcgtccgcga cgccgaaaaa gctcgctcca tcagggtggt gcatccgcct tcggccctcg
     1621 gccgggtcaa acggaagctc catgagctgt tccgcaccga cgaaaacgtg ttccatcgga
     1681 tcatctacgg tctggcctgg acaacaaaag accgctgtcc atggcttgcg gggccgaccg
     1741 tcgagtcggt caaggcggcg ttggatcggg ccgccgttga gcccggctgg acgatcctcg
     1801 accggcccat caagcctgat ctcgtcgtcg ttgtggcaac cccggccaaa ccattccgcg
     1861 ctacagacgg tgcggagatt tcaatcgata tccctcagca cgccccccgc cgcgcggtga
     1921 tcagcggtcg gcgacggtgg gcaagggatt cgtggccacg accgatttgg agatgctcaa
     1981 cgtgctggtc gaccgtctgg tccgcagctc gcggcgtctt gcataaaggg taacgcagat
     2041 gataaagacc gcattcgcct tgttgctcgg attgctcccg gctctgctta ttgccgcagg
     2101 ctgcgagaaa ggagctacgt cgccgcgcgg ccaagactca ccacctgcta atccgagcac
     2161 ggtacctgcc ctgcgtgatg gagagcagcc aagtgacggc gtcccgatcg aggaactagc
     2221 cgcagccttc ccctcaggat atgagcttca aaacaaacga ttgagccacg tcgggctggg
     2281 ttatcactac ctgtataagg ccgccaacac ggacaaacgc ctcgcggtta ccgtcggcct
     2341 cttcgggaat tccgcaaagg cccgcaacgc agccctcctc tcgcatgacc tcatgcccgt
     2401 gaagccgcgc ggcctcgatg gtatcgctga cgaggcatgg atctgggatg aagacgacaa
     2461 ggcggcggtg cgattccgcc tgggccggct gctctgccgc gtcggcgggg acgtgtctgt
     2521 gcccgaggcc cgccagatcg cggaacggat ggtcctgcaa ctttccgatc ggttgccatt
     2581 gttgcgacga gacgacggcg atgtgtcttc ggcggcttgg tccggcattc ctgctcacat
     2641 gcgtcccggt gagagcgcag ttgttcggtt caatgccgga acggtcaccg ccccagagcg
     2701 catggaggcc ggagtctggg cctcggccgg ccgggtatcc gcgaagtccg aaggcacgtt
     2761 ccgcttcacg gctccggtcc gaccgggacc tttgcagatc cggtgctttg ccacatcgca
     2821 ggcaaacagg gttgaggttt cagtctttga cgtgcaggtc aacattcaag aaaggaagcc
     2881 tggcaatgaa cacaaaacac gaacatctgc agggaactga gacgaccgtc agccccgacg
     2941 gcgccggggt tgagggctac ggtggttcag agcggcccgg accggacgcc tgcgaggcaa
     3001 cgacctacga acggccggtc ctgatccgcc gcgacgagtc gcgggaagga gatttctggt
     3061 gtgactgagg cgcctccggg taacgagaag ccacgctttc gccgcgaacg cgtacggctg
     3121 caagaaacga ccaccggcct gcttgtctat gacatcgagt cggactcgct gtttcacgcc
     3181 aacaggacgg caagcatggt gcttgaactg ctcgacggaa cccgttcctg ggcgcaaatc
     3241 gcccacgctc tcgccgatcg gcacgggtgg gcaacggccg aaatcgcggc cgacttgtcg
     3301 acgttcgcaa gagagctcat gaccgtcggg cttctggaag aaccgccatg gaattcatgc
     3361 atccaccctt gagggtgagt atcgatgtca caggggcatg caacctctcg tgtcgccact
     3421 gccgacacgg atcgcaagcc tgcgacgagc tgacgcttgc cgagatctca tccgtgattg
     3481 atgacgtcgg gcaaatggga gtgttcaggc tggtgatctc cggcggcgaa cccttccttc
     3541 gtcgggatat cctggaaatc ctgctacgag cgctgcgaac ggaggtgggg cgagtgttcg
     3601 tctcaactaa cggcacctgc atgccgaagc gcgtgccgga tagactcacc gcgtttcgcc
     3661 gtcgcctgac tttcaagatc tcacttgacg gagcacctga tttccacgat gcctggcgcg
     3721 gccggaaagg cgccttcgca aaggcatcgg cctcgatccg ccttttgaca caacgaggct
     3781 ttgacgtgca ggtgaccaca accctgatgc catccaacct cgattccctg tcctggctgc
     3841 tcgaatacgt tgccgaaaca ggaagctcgc ggcacgccct cgttgaggtt attcccgttg
     3901 gccgggccgg tgtcgagatg gcgttgagca ccgacgagag aatccgggct gccgaggcaa
     3961 ttcggtttgc cagatcgaga ggcgggcact gcgagattat ctccaagatc gcgtttgtcg
     4021 acgggcggtg ctcggggttt ttctgttccg gcgggatcga ggagtgcggg gtgttgtcgg
     4081 atggacgaat cgttggttgt cgcctgatgc ccgatgtcgt ggcaggcaac gtcagggaga
     4141 tgcccttgag tcgggtctgg tccagctcgg agcggttcag cctgtttcgc ggtatcacac
     4201 cggcaagatt gcagaaaaca tgccgcgatt gcgaaatggc tgccgcct
//
LOCUS       DAOTPW010000362         4230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16978_length_4230_cov_4.357126, whole genome shotgun sequence.
ACCESSION   DAOTPW010000362 DAOTPW010000000
VERSION     DAOTPW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16978_length_4230_cov_4.357126"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1229)
                     /locus_tag="P5316_20855"
     CDS             complement(183..1229)
                     /locus_tag="P5316_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019026063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRR87463.1"
                     /translation="MSCSEADDRADPRGASVEWFRHAGFGLFMHYGLYSQLGRGEWVM
                     YRDKIPVAEYVKLKDTFTAEKFDADHMVDLALAAGMKYVNITSRHHDGFCLFRTSQTD
                     YNSVSSPAKRDLVGELAEACRKKKVGLFLYYSYALDWKHPYFFPRDERCPVARPAYDK
                     PEPTYLFRKDEDFRHYLDFVHAQMRELLTQYGPLAGLWLDPLMGYYGRPELFPLKETY
                     GLIRSLQPGCLISFKQGANGDEDFVAPERKAAALKRGGDAAVAVWEKNRGKPVEICDT
                     LQPAAWGYDKSAEGKHKTGDDVMRMLADARAQDANLLLNTGPLADGSIHPDDEATLRE
                     VGRRLRNQAATRKR"
     gene            complement(1564..3681)
                     /locus_tag="P5316_20860"
     CDS             complement(1564..3681)
                     /locus_tag="P5316_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87464.1"
                     /translation="MCGKTVSRYWFGAAATWFSACAAMAADAGVLGAYFRADESFPDY
                     AVFWHEGWTTPDGEIDNARYEGFKEPLGGSLHVFLKNTTSKPLVIEDVLLDGISLKKV
                     IVFSEQRKNRKPASIYFAGLPQDTMDHLFALGEPIWWKVEPNKVSPGQTCEVVVRLRT
                     VPRNPSLPLTLIHPGGKIEVSVPVRTKGARVAGASFSPGRDVLYLYFRHQGQSGAAPA
                     RILIDGTDVTASAVIGRDASAAVVPAVIRLSRPMEPASLHCVQGVFDDGETASAGVRV
                     WADEFAYGMWGGKPGKANDLSVGENYVTELVAHNINVQMPQVGSPAVQTFYKSEAGQR
                     RLKELGFRAVIDEHQKWGQKDPYLFFIHDEPDCADFRFEGIPEDKKIGALAMWTLQRS
                     GELRKADAGRLQLLNVDMTYKPQNWYIYGQVPDVLAADPYYQNRLRTAYQEHPERIKA
                     YSKATYVYAVADVCRSACEPKPLHIILYSCSHVDNRANRRFRFPTPPEKRIEVFYALA
                     AGAQGLSYWWYTPADKGEKAAYGVGAAVQDGNAAAAALYREIGLLGAEVRTAGPLFLT
                     GCPAEVSLKTEGAIWTRTLLAGVDSLVLLVVNDQYTNHDEGTDIRPVENAAVTVKTPA
                     WLRAADAFEITSQGTREVKWASTGSQLRLELGKVDVTRLVIITADKSLRTELQKRYTE
                     QFADRVRALMQQGAGPATPKARK"
     gene            complement(3707..>4230)
                     /locus_tag="P5316_20865"
     CDS             complement(3707..>4230)
                     /locus_tag="P5316_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87465.1"
                     /translation="FDRLFMEAFIRPGLDPENWGGAHGNSTPAWGILQTGPAEMSIYW
                     TEHYENYPENTGRIPQIRRGTMRLDGFVSVNAPYEGGEFVTKPLVFEGSKLVLNMSTS
                     AVGSVKVGIEDADGQPIPGLGLNDAVEIWGDEIERIVNWKGGPDVSRLAGKPVRLRFV
                     MKDADLYSIRFRS"
BASE COUNT          806 a         1435 c         1180 g          809 t
ORIGIN      
        1 cagcgaccga gacctgccgt atctgctggc cccagaatcc tttctggggc ctttcggggg
       61 gccacatggt agggcagcgt cgaggtcgcc gcagcgaccg agacctgccg tatcctttgg
      121 agtggcaggt ctgcaccacc tgtgccggct tccaccatgc cctacgttgg ttacgaacgc
      181 tctcagcgct ttctcgtggc cgcctggttg cgcaaccgtc tgccgacctc gcgaagtgta
      241 gcctcatcgt ccggatgaat cgagccgtcg gccagaggcc cggtgttgag cagcaggttg
      301 gcatcctggg cccgggcgtc ggccaacatc ctcatcacgt cgtcaccggt cttatgtttg
      361 ccttcggcgg acttatcata accccatgcg gccggctgca acgtgtcgca gatttccacc
      421 ggcttgccgc gattcttctc ccatacggcg acggctgcat cgccgccccg cttgagggcc
      481 gccgccttgc gctccggcgc gacaaagtcc tcgtcgccgt tggctccctg cttgaaagag
      541 atcagacagc ccggctggag cgagcggatc aggccgtacg tctccttcag cggaaacagt
      601 tccggccgcc cgtagtaccc catcagaggg tcgagccaga gtccggcaag cggcccgtat
      661 tgtgtcagca gctcgcgcat ctgagcatga acgaagtcga ggtaatgtcg aaaatcctcg
      721 tccttgcgga acagataggt cggctccggc ttgtcatagg ccggtcgtgc gaccgggcag
      781 cgctcgtcgc gagggaaaaa gtacggatgc ttccagtcta aggcatacga gtagtacaga
      841 aacaaaccga ccttcttctt gcggcacgcc tcggccagct ctccgaccag gtcacgcttg
      901 gccgggctgc tcacgctgtt gtagtccgtc tgtgacgtgc gaaacaggca gaacccatcg
      961 tgatgacgag aggtgatgtt gacgtacttc atccccgcgg ccagagccag atcaaccatg
     1021 tggtcggcat caaacttctc ggccgtaaac gtgtccttga gcttcacgta ctcggccacg
     1081 ggaatcttgt cccgatacat cacccactcc ccgcgaccta actggctgta gagaccgtaa
     1141 tgcatgaaca gcccaaatcc ggcgtggcgg aaccattcga cggaagcccc gcgaggatca
     1201 gcccgatcat cagcctccga gcagctcatg tacgccggca ccgccgacgt gcagccggat
     1261 atgcccggca gcagccagaa agccgcagcc gatccggagg atttcagcag cgccctcctg
     1321 ctgacgcgtt gagacccctg cccaggggat gaagcgttcg tctgttgcat acgacctcca
     1381 acgaactgac ggtgaccgtg cgccagaggg gcacaggtat gtaacctgag gcgtgagccc
     1441 caggtatcgg tacggccaac acctatcaag cccccaaggg gcggcagcgg attacctgca
     1501 ccaggacccc acggatccta ccggggcttc tggcgatcgt ccgctcgaca agctgcacac
     1561 tgttcacttg cgagctttcg gcgtcgccgg cccggccccc tgctgcatga gggccctgac
     1621 cctgtctgca aactgctccg tataacgctt ctgcaactcc gtgcgaaggc ttttgtctgc
     1681 ggtgatgatc accagacgcg tcacgtcaac cttgcccagt tcgagtcgca actgcgaccc
     1741 ggttgacgcc catttcacct ccctggtgcc ctggctggtg atctcgaagg catcagcggc
     1801 tctcaaccac gcgggcgtct tgactgtcac ggcggcgttc tcaaccggcc gaatgtcggt
     1861 accctcatcg tggttcgtat actggtcgtt gaccaccaac aaaaccagcg agtccacacc
     1921 ggccagcaac gtccgagtcc agatcgcccc ttccgtcttg agggacacct cggcagggca
     1981 acccgtcaaa aacagcggcc cggccgtgcg gacctcggcg cccaacaggc cgatctctcg
     2041 gtacaacgcc gctgcggcag cattgccatc ctgaacggcc gctccaaccc cgtatgcggc
     2101 cttctcaccc ttgtccgccg gcgtgtacca ccagtacgaa agcccttgcg cccccgccgc
     2161 aagcgcatag aagacttcaa tccgcttctc aggaggcgtc ggaaagcgaa atcggcgatt
     2221 ggccctgttg tcgacgtggc tgcacgaata cagaatgatg tgcagcggct tgggctcgca
     2281 cgccgaccga cagacgtccg ccaccgcata aacataagtc gccttgctgt aagccttgat
     2341 ccgctcaggg tgctcctggt acgccgtccg gagccgattc tggtagtaag gatccgccgc
     2401 cagcacgtcc ggcacctgcc cgtagatgta ccagttctgt ggcttgtagg tcatgtccac
     2461 gttcagcaat tgcaggcggc cggcatccgc cttgcgcaac tcgccggatc gctggagcgt
     2521 ccacattgcc agagccccga tcttcttgtc ctcgggaatg ccttcgaagc ggaaatcggc
     2581 acagtccggc tcatcgtgga tgaagaacag gtacggatcc ttctgccccc atttctggtg
     2641 ctcgtcgatc accgcgcgaa aacccagctc tttgagccgc cgctggccgg cctcgctctt
     2701 gtaaaacgtc tgtacggccg gcgaccccac ctgcggcatc tgcacgttaa tgttgtgcgc
     2761 aaccagctcg gtcacgtagt tctcgccgac gctcaaatcg ttcgccttgc ccggcttgcc
     2821 gccccacatg ccataggcga attcgtcggc ccaaacccgg acgccggctg aggccgtctc
     2881 accatcgtca aacaccccct gtacacaatg aagagaagcg ggctccatcg gccgggacaa
     2941 gcgaatgacc gccggcacca cagccgcgct ggcatctcga ccaatgacgg ccgacgctgt
     3001 aacgtccgtt ccatcgatca gtattctcgc gggcgcggct cctgactgtc cttggtgacg
     3061 gaagtacaga taaagaacgt cgcgaccggg cgaaaacgag gctcccgcca cccgagcacc
     3121 cttggttcgg acgggaaccg acacctcaat cttgccgccc ggatgaatca gcgtcagcgg
     3181 cagactcgga ttcctgggca cggtgcgaag ccgcaccacc acctcacatg tctgccccgg
     3241 ggaaaccttg ttcggctcaa ccttccacca gatcggctcg cccaaggcga aaaggtgatc
     3301 catggtgtcc tggggcaggc ccgcgaagta gatgcttgcc ggcttacggt tcttgcgttg
     3361 ctccgagaag acgatcacct ttttgagact gatgccgtcc agcagaacgt cttcgatcac
     3421 cagcggtttc gacgtggtgt ttttgagaaa gacgtgcagt gaccccccga gcggctcctt
     3481 gaatccctca tacctggcgt tgtcgatttc gccgtccggt gtcgtccagc cctcgtgcca
     3541 gaagacggca tagtcgggga acgattcgtc cgcacggaag taggcaccca acacgcccgc
     3601 gtcggcggcc atcgcggcgc acgccgaaaa ccacgttgca gcagccccaa accagtatct
     3661 gctcaccgtt ttgccgcaca tcgacaaacc cccttctgag ccgggatcag gaccggaatc
     3721 gaatcgagta caagtcggca tccttcatca cgaatcgcaa ccgcaccggc ttgccggcca
     3781 gcctgctcac gtcaggccca cccttccagt tgacgatccg ctcaatctcg tcgccccaga
     3841 tctcgaccgc atcattcagt cccaagccgg ggatcggttg cccgtcggca tcctcgatgc
     3901 cgaccttcac gcttccgacc gccgaggtcg acatgtttag caccagcttt gacccctcga
     3961 acaccagcgg cttggtcacg aactccccgc cttcgtacgg ggcattgacg ctgacgaacc
     4021 cgtccagtcg catcgttcct ctacgaattt gcgggatccg gccggtgttc tcaggatagt
     4081 tttcgtagtg ctccgtccag tagatggaca tctccgccgg cccggtctgc agaatgcccc
     4141 acgccggcgt gctgttgccg tgggctccgc cccagttttc cggatccagc cccggccgaa
     4201 tgaaggcctc catgaacagg cggtcaaaat
//
LOCUS       DAOTPW010000363         4229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_16983_length_4229_cov_5.940345, whole genome shotgun sequence.
ACCESSION   DAOTPW010000363 DAOTPW010000000
VERSION     DAOTPW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16983_length_4229_cov_5.940345"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="P5316_20870"
     CDS             <1..316
                     /locus_tag="P5316_20870"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR87466.1"
                     /translation="EVQARDDRNDKDLTDVSGAIFNRIAAAGRFARPPGQWNRLKIIC
                     MGRRLRVELNGHVTVDADMDSVPAKGNDPPMRNVPDAGYIGLQNHGTPAEFRDIRIRE
                     LR"
     gene            537..2567
                     /locus_tag="P5316_20875"
     CDS             537..2567
                     /locus_tag="P5316_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87467.1"
                     /translation="MIPNLVEQVGQAAVYVVNAGGGAKLNAVRELMIVLLGIEAGAAG
                     GGRDACWLQLQVVKHNGVTLTATVLVDDPLLTPEHTGKARVLRYIYQEGDHQPIEYVN
                     GIRGGAFTTVFGLLPNFFPAFEKDDLEKTLFPDSGKYLGHKIEKKATRKVDAHVPRNV
                     LRLELDPEMLVGTGRNCREVEGRRVWTGDEYQYRTFEKADYDEMIDAGINYFWVDRRQ
                     EEWVRNRPVFFLKWHGNKPDYPEMFYRANCRGISMFMDEPAVHMAWFFQRHGPFTKTL
                     SGPAGAARFLEARVRDDIKTGNYSQLRLHDMLRNQYDLGDMTIRENHYPAWETMEWSV
                     YYQMKAGLPGAVHEGRYTNKADVNVFNAEYDTRIPDTPENNMRIRYAWFRGAARMFKG
                     DWGTAIYGQCEESVAPLAVTMAWDMGARYVWYWTSDRLHHMPYAEQLALTRHLREHAR
                     KNPRKPLAELNRAARAVILLPDGYVLTGFHMYETNVFHLDRINEKGLRYRDVLKPAAL
                     QMERYLKESIPFDIIYNDGTPGWEEYDEAVIVGEDGSARWYKKGQHVGDPPLPQRTPA
                     ELASKAPVIRIRHEQPAADAAPLSVKLIADIDNDGKPVGLRTRDWGTPNWTYMEAFWR
                     VYDDLAEPRFHIGRTLEHTFRKPGAYRITAWTCNEDGVAGRTETTILVEDGK"
     gene            2564..3166
                     /locus_tag="P5316_20880"
     CDS             2564..3166
                     /locus_tag="P5316_20880"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRR87468.1"
                     /translation="MSLHEIERIGEVYVARSAIIVGDVTLGEGCNIWHHVVLRGDVAP
                     IRLGKRVNVQDGSVLHCNHDIPLEIADDVAIGHLACVHCKRVGPNTLIATRATVLDNC
                     EIGQDCMIGAGAVVPPGTVIPDGSVALGMPAKVIRPIKDRDREYIRRVLTYYQDLARR
                     HAEGEFKSFTSEETGKTASEAGPGRRESRRTFHSRESPGA"
     gene            complement(3308..>4229)
                     /gene="larA"
                     /locus_tag="P5316_20885"
     CDS             complement(3308..>4229)
                     /gene="larA"
                     /locus_tag="P5316_20885"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRR87469.1"
                     /translation="VVANYRGIQHDGNDDASLVKLGRTRFDHDIRVNRHFVEADVRIL
                     TGFIEPHFFAGFSGGPKGVLPSIAGAESVLDNHGAEMIGHPKSTWGITDGNPIWEEML
                     EAARMTKPTFLLNVAMNRDKQITGVFAGDLYEAHKAGCAFVKSCAMVPVAEPFDVVIT
                     SNSGYPLDLNLYQTIKGVSAANQVVREGGAIIAVSQCWDGIPEHGCYGKLLRESKSPA
                     ELLARVRSPGFSSPDQWQVQVQTMIQARAEVYIRSDGLTDEQITQCLLKPCRKVEDTL
                     AELASKAHGNRLSICVLPEGPITIPYVARG"
BASE COUNT          869 a         1352 c         1306 g          702 t
ORIGIN      
        1 tgaggtccag gcccgcgacg accgcaacga caaggacctc accgacgtca gcggcgccat
       61 attcaatcgc atcgccgccg ctggcaggtt cgccaggccg cccggccagt ggaaccgcct
      121 taaaatcatc tgcatgggcc gccgtcttcg ggtggaactc aacggccacg tcacggtgga
      181 tgccgacatg gacagtgttc cggccaaggg caacgaccca ccgatgagaa acgtgcctga
      241 cgcgggatat attggcctgc agaaccacgg taccccggcc gagttccgcg acatccgaat
      301 ccgagagctt cggtgagttc ctcaggtcga tcatagcgtg gaggttaaga cacacctgca
      361 atcggcccgt cctgatcgtg gcattcgggc agaccttggc gggtgctgct ggcagcttcg
      421 ggccttcctg gccgaagcga cagcttcggc gcggagcagg ccgctcgcca gtgcccaact
      481 gacgtggcgg agaaaaacag cacaaacgcg agaacaagtt ggcaggaggt cttctgatga
      541 tcccaaacct tgtcgagcag gtcgggcaag cggctgttta cgtggtcaac gccggcggcg
      601 gtgccaaact caacgcggtc cgcgaactga tgattgtgct tctgggcatc gaggccgggg
      661 cggccggcgg cgggcgggat gcctgctggc tgcaactgca ggtcgtcaaa cacaacggcg
      721 tgactctgac cgcaaccgta ttggtggatg atcccctgct aacccccgag cacaccggca
      781 aggcccgcgt actccggtat atctaccagg agggcgacca ccagccgatc gaatacgtca
      841 acggcatacg cggcggcgcg tttaccactg tcttcgggct gctgcccaac ttctttccgg
      901 ctttcgagaa ggacgatctg gagaagacgc tctttccaga cagcggcaag tacctgggcc
      961 acaagatcga gaagaaagca acgcgcaagg ttgacgcgca cgttccccgc aacgttctgc
     1021 gcctggaact cgatccggaa atgctcgtcg gcaccggccg caactgccgc gaggtcgaag
     1081 gccgccgcgt gtggacgggt gacgagtatc aataccgcac tttcgagaaa gccgactacg
     1141 acgagatgat cgacgcgggc atcaactact tctgggtgga tcggcggcag gaggaatggg
     1201 tccgcaaccg tccggtgttc ttcctcaaat ggcacggcaa caagcccgac tacccggaaa
     1261 tgttctaccg ggccaactgc cgcgggatca gcatgttcat ggacgagccc gcggtacaca
     1321 tggcctggtt cttccagcga cacggcccgt tcaccaagac gctctcaggc ccggccggag
     1381 ccgcccgctt cctggaagcc cgtgttcgcg acgacatcaa gaccggcaac tactcgcagt
     1441 tgcggctgca tgacatgctc cgaaaccagt acgacctcgg cgatatgacc atccgcgaaa
     1501 accactatcc ggcgtgggaa acgatggagt ggtcggtgta ctaccagatg aaagccggcc
     1561 tgccaggcgc cgtgcatgag ggacgctaca ccaacaaggc cgacgtcaac gtgttcaacg
     1621 ccgaatacga cacacgcata ccggacacgc cggaaaacaa catgcggatt cgctacgcct
     1681 ggttccgcgg ggcggcgcgg atgttcaagg gtgactgggg cacggccatc tacggccagt
     1741 gcgaggagtc ggtcgccccg ctggccgtta ccatggcgtg ggacatgggc gcccgctacg
     1801 tgtggtactg gacctcggac cgcctgcacc acatgccgta cgccgaacag ctcgccctga
     1861 cgcggcatct gcgcgagcat gcccgcaaga acccgcgcaa acccctcgct gagctgaacc
     1921 gggcggcccg ggcggtgatc cttctgcccg acggctacgt cctgaccgga ttccacatgt
     1981 acgaaaccaa cgttttccat ctcgaccgga tcaacgagaa aggccttcgg tatcgcgacg
     2041 tcctcaagcc ggcggcgctg caaatggagc ggtaccttaa ggagagtatt ccgttcgaca
     2101 tcatctacaa cgacggaacg ccaggctggg aggagtacga cgaggcggtg atcgtcggcg
     2161 aggacggctc cgcccgctgg tacaagaaag ggcagcacgt cggcgaccct ccgttgccgc
     2221 agcggacgcc ggccgaactg gcctccaagg ccccggtcat ccgcatccgc cacgaacagc
     2281 cggccgcaga cgccgccccg ctgagcgtca agctcatcgc tgacattgac aacgacggca
     2341 agccggtcgg gctgcgcacc cgcgactggg gcacgccgaa ctggacctac atggaggctt
     2401 tctggcgggt ctatgacgat ctggccgaac cgcgtttcca catcggccgg acactcgaac
     2461 acactttccg caaaccggga gcctaccgaa taacggcttg gacctgcaac gaggacggcg
     2521 ttgccgggcg caccgaaacg acaattctgg tggaggacgg caagtgagcc tgcacgagat
     2581 cgagcgaatc ggcgaggtat atgtggcccg cagcgcgatc atcgtcggcg atgtcacgct
     2641 cggcgagggc tgcaacatct ggcaccacgt tgtgctgcgc ggcgatgtgg ccccgatccg
     2701 gctgggcaag cgcgtcaacg tgcaggacgg ctcagtgttg cactgtaatc acgacatccc
     2761 tctggagatc gccgatgacg tggccattgg tcacctggcg tgtgtccact gcaaacgcgt
     2821 cggtcccaat acgctcatcg ccacgcgggc gacggtgctc gacaattgcg aaatcggtca
     2881 ggattgcatg atcggcgcgg gggcggtggt tccccccggc acggtcatcc ccgacggctc
     2941 cgtcgccctg ggcatgccgg ccaaagtcat ccgcccgatc aaggatcgag accgcgagta
     3001 catccgccgc gtcctgacct attaccagga ccttgcccgc cgccatgcag agggcgaatt
     3061 caaatcgttc acgtccgagg aaaccggaaa aacggcctcg gaagcagggc ccggaagacg
     3121 cgagtcacgt cgcacgttcc acagcagaga gagtccggga gcgtagtttc gagaggcaag
     3181 ctcgtgagcc acatgccgga agagggccga cgagggcgcc gaccccactc gggccgccca
     3241 acgatctggc taatagctaa cagctcaaag ctctctcttc tgaccactga caactgacca
     3301 ctgacaacta cccccgtgcg acatacggga tggtaatcgg cccctcgggc aacacgcaga
     3361 tggacaatcg attgccgtgg gccttcgatg ctaactcggc cagcgtgtcc tccaccttgc
     3421 ggcaaggttt cagcagacac tgggtaatct gctcgtcggt caaaccgtca gaccggatgt
     3481 aaacctcggc tcgggcctgg atcatggttt gaacctgcac ctgccactgg tcgggcgagc
     3541 tgaaaccggg cgaccgaacg cgagcaagca gctcggccgg gctcttggac tcgcgaagca
     3601 gtttcccgta gcaaccatgc tcgggaatgc cgtcccagca ctgcgaaacg gcgatgatcg
     3661 caccaccctc ccgaaccact tggttggccg ccgagacccc cttgatcgtc tggtagagat
     3721 taaggtccag cggatagccc gagttgctgg tgatgactac gtcgaacggc tcggctaccg
     3781 ggaccatcgc gcaactcttg acgaacgcgc aaccggcctt gtgagcctca tagaggtcgc
     3841 cggcaaacac tccggtgatc tgcttgtcgc gattcatcgc cacgttgagc aggaaggtcg
     3901 gcttggtcat ccgggcggct tcgagcatct cttcccagat cgggttgccg tcggtgatgc
     3961 cccaggtgct cttgggatga ccgatcatct ccgccccgtg attgtcgagc acgctctccg
     4021 ccccggcgat ggagggcaac acacccttcg gcccgccgct gaatccggcg aaaaagtgcg
     4081 gctcgatgaa cccggtgagg atgcgaacgt cggcctcgac gaaatggcgg ttgacgcgga
     4141 tgtcatggtc gaagcgggta cggccgagct tgaccaggct ggcgtcgtcg ttgccgtcgt
     4201 gctgaatgcc gcggtagttc gcgaccacc
//
LOCUS       DAOTPW010000364         4211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_17070_length_4211_cov_4.641963, whole genome shotgun sequence.
ACCESSION   DAOTPW010000364 DAOTPW010000000
VERSION     DAOTPW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17070_length_4211_cov_4.641963"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..363)
                     /locus_tag="P5316_20890"
     CDS             complement(52..363)
                     /locus_tag="P5316_20890"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HRR87470.1"
                     /translation="MPRWLSKWFERHRHPASLVLHLVGIPLTVAAVLLAVVQFCQWRW
                     DLWWRPTSLLIAGYLLQWIGHRIEGNDMGELILVKKRLGRPYVAVSPKYARKAFRQSD
                     L"
     gene            complement(602..1033)
                     /locus_tag="P5316_20895"
     CDS             complement(602..1033)
                     /locus_tag="P5316_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87471.1"
                     /translation="MALTGHDRTKVSAARFHDAFSCFDRYAGQAFASAFGSVAHFVAQ
                     HRMKVRISDAVPGRRSFRFALPDGSRVTISCSIEGIDQLESTSECWMPQIGPGSGARS
                     TTGLRDADRRWGEACMLMALDRSVRRWVENHNWAPKGQKHA"
     gene            complement(1298..>4211)
                     /locus_tag="P5316_20900"
     CDS             complement(1298..>4211)
                     /locus_tag="P5316_20900"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRR87472.1"
                     /translation="DRCYWPKQGGVQSMPDCTCRGMGVKVAWNMKANEKNCVSFSGCF
                     FSLVLLPLAVWCGSSAAASAGQAEADSVHPVYRLNSSHWHALGLFEWTKENRRFWGDR
                     PLPPDNRFDTSATYEGLAGKVAWIEVPEWDQDNKVHEIREKLKPRETRHSAAAYLYRR
                     IDSPAETDAILYLGFDDGLVLNLNGEIIFERFFYRNTEPRQEAIPIRLKQGRNDLLLK
                     IIDKDNTGGSAFCFDIRPSLPPDEHVRLLEDLITRQSNEPVRALDTRCRLMHLYRQTG
                     QLAKADATAQEVLSIQASVAELKEAAQRFLETRPGGIGRITGYTVEAQRATFHTERGK
                     LHVGYWRDTAPRVTFVPTDTAWPYPPSHRPLPVGWETPNKSGINAVTDAEDRFVVKGI
                     AVPVEVFKDGSGIAFLHEGRRRFVELGMRPSLKRVVWSQAKTFKGPFEEIVFHLDTRE
                     GVFGMGNRYDALNRRGRLNIIGNCDRAFGESHFTLPHFLTQGRDALFINSFGDGEIGV
                     DRPEAENMAVCQLQEDVIDLFYFAGPPRALVRQYADLTGHTVMPPDWTLGVWMSRNSY
                     ENEAVVLDVARKLREHKVPAHVLVLEAWREDGKDWMSWNTERWPRHQAMCKTLHEMGF
                     KIVLWTMQFHIYNLARPQPYEQEAFDNRYFVMDGERPWGYEQGQEKNSYIVDFFNPAA
                     CAWWERQYRRLFDPVIGIDGLKTDIGENNGGTTWQGWKNINNIYALGYLRCAWDMTKD
                     ITGEGMVFARTGTVGTQRYPILWAGDHSAWFQGMQEAFNAMMNAGLSGYAWTSFDIGG
                     LYGDLDKETYVRMAQMGAFCPIMQVHGQGRREPYDFGDYGEQATEVFNRYATWYMRLK
                     PYRIAAGREACETGVPIIRPLWMDHPDDPECYEAEYEYLFGPDVLVAPIVSYNHTRKV
                     YLPRGQWIDWWTGERIVGGRYLRVTMPLEQVPLYVKPGSLVLRLSPGSGGGNEKSE"
BASE COUNT          801 a         1333 c         1243 g          834 t
ORIGIN      
        1 cgattgggct tcaaccgcat gcaatgcgcg gtcataagtc ggaaaggtcc gctagaggtc
       61 tgactgccta aaagcctttc gtgcgtattt cggtgacacg gccacatacg gccggcccag
      121 ccgtttcttc accaggatta actcccccat atcgttgcct tcgatccgat ggccgatcca
      181 ttgcagcaga tagcccgcaa tcagcaagct cgtcggccgc caccacagat cccacctcca
      241 ctggcagaac tgtacgacgg ccaggagcac cgcggccacc gtcagcggaa taccaaccag
      301 gtgaagcacc agcgacgccg gatgccgatg ccgctcaaac catttggaga gccatcgagg
      361 cataactatg aggccgtcct tgtgctgcca ccctttgaga tcgacggttc tgcagactcg
      421 gcggctggtt cttgcacctt gcccgtcggt gcgccgaaaa cgccaccagt gccgggtccc
      481 cggttgttgt cgggggatta tatccctgcg gcggtttgcg agcgaaatga agccctgccc
      541 cgatcgattg accggtaatg cgtcacggga aacgacctgg gggggctcgt cctcgtggtc
      601 ttcaggcgtg cttctggccc ttcggtgccc aattgtggtt ctcaacccat cgacggacgg
      661 acctatccag ggccatgagc atgcaggcct cgccccatcg gcggtcggcg tcgcgaaggc
      721 cggtggtcga tcgcgcgccc gagccgggcc cgatttgcgg catccagcat tcggaggtag
      781 actctagctg atcgattcct tcgatggaac aggagatggt gacccggctc ccgtcgggca
      841 gggcgaaccg gaaggagcga cggccgggca ccgcgtcgct gatccgaacc ttcatccggt
      901 gttgcgcgac gaagtgagcg acggaaccga acgccgaagc gaatgcctgg ccggcgtagc
      961 gatcgaagca ggagaacgca tcgtggaatc gcgccgccga caccttggta cggtcatgtc
     1021 ccgttaatgc catctttccc cacaaaggcg cgatccgcct gcccacccgc cggcgcaaca
     1081 cctgatgatc agtgcccaca aggcccccaa cgaagggcca cgacagaacc tacgatataa
     1141 cttcccgcgg acccaaccgg tcgcgttgcc ttatgggtcc tccgctcgcg ggcccccggg
     1201 gcgcgcccgc cccgtcacga acagacaaac agaatgatag acccaatact ccggatgcga
     1261 tcgcgaagat cgcaaaggac gcctcttccc cggttcccta ttccgacttc tcattgccgc
     1321 cgcccgaacc ggggctgaga cgcagtacca acgagcccgg cttaacgtag agcggcacct
     1381 gttccagcgg catggtcacg cgcaggtaac gtccccccac gatacgctca cctgtccacc
     1441 agtcgatcca ctgcccgcgc ggcagataca ctttacgcgt atggttgtat gacacaatcg
     1501 gagctacgag cacgtccgga ccgaacaagt actcgtactc ggcctcgtaa cactcgggat
     1561 catcggggtg gtccatccac agtggcctga tgatcggcac gccggtttcg caggcctccc
     1621 ggccggcagc gatccgatat ggcttgagcc gcatgtacca cgtggcatac cgattgaaca
     1681 cctcggtggc ctgctcgccg taatcgccaa agtcgtaagg ctcccggcgg ccctggccat
     1741 ggacctgcat gatcgggcaa aatgctccca tctgggccat gcgtacatac gtttccttgt
     1801 cgagatcgcc gtagaggccg ccgatgtcaa agctggtcca ggcatagccg gaaaggccgg
     1861 cgttcatcat cgcattgaac gcttcctgca ttccctggaa ccaggcgctg tggtcgcccg
     1921 cccagagaat cggatatcgt tgcgtgccca ccgtgccggt gcgggcgaag accatcccct
     1981 cgccggttat gtctttggtc atgtcccagg cgcagcggag gtagccgaga gcgtagatgt
     2041 tgttgatgtt cttccacccc tgccaggtcg tgccgccgtt gttctccccg atgtcggtct
     2101 tcaggccgtc aatgccgata accgggtcga agagccggcg gtactgccgc tcccaccacg
     2161 cacaagcggc gggattgaag aagtcgacaa tgtaagagtt cttctcttgg ccctgctcgt
     2221 agccccatgg tcgctcgccg tccatgacga agtatcgatt gtcgaaagct tcctgttcgt
     2281 acggttgcgg tcgggccaag ttgtagatgt ggaactgcat ggtccaaagg acgatcttga
     2341 aacccatctc atgaagagtt ttgcacattg cctggtgacg tggccatcgt tcagtgttcc
     2401 aggacatcca atcctttcca tcctcacgcc aggcctcgag caccagtacg tgggccggca
     2461 ccttgtgctc gcggagcttg cgggctacat cgagaactac cgcttcgttc tcgtaggagt
     2521 tgcgggacat ccacaccccg agcgtccaat cgggcggcat gacagtatga ccggtgagat
     2581 cggcgtattg acgcacgagg gcccgaggcg gccccgcgaa atagaacagg tcgatcacgt
     2641 cctcctgcaa ctggcagacg gccatattct ctgcctccgg tcggtccacg ccgatctcgc
     2701 cgtcgccgaa gctgttgata aacagcgcgt cgcgaccctg ggtcaggaaa tgcggaagag
     2761 tgaagtgcga ttcaccaaac gcccggtcgc agttcccgat gatgttgagt ctgccccggc
     2821 ggttgagggc gtcgtaacgg ttccccattc caaagacgcc ttcccgcgtg tcgaggtgaa
     2881 acactatttc ctcgaatggg cctttgaatg tcttcgcctg tgaccatacc acccgcttga
     2941 gactggggcg catgcccaac tcgacgaatc gcctgcgacc ttcgtgcagg aatgcgattc
     3001 cggaaccgtc cttgaagacc tccaccggga ctgctatccc ctttacgaca aaccggtcct
     3061 cggcatcagt cactgcgttg atgccgctct tgttgggcgt ctcccagccg acgggcagcg
     3121 gacggtgcga cgggggatag ggccaggcgg tgtccgtcgg aacgaaagtc acccgcggag
     3181 ccgtgtcccg ccagtagccg acgtgcagct tgccccgttc cgtgtggaaa gtggctcgct
     3241 gtgcttcaac ggtgtaaccg gtgatacggc cgatgccgcc gggccgcgtt tcgagaaacc
     3301 gctgggcggc ttccttcagt tcagccacgg aggcttggat ggacaaaacc tcctgtgcgg
     3361 tggcgtcggc ttttgccaat tgcccggtct ggcggtagag gtgcatgagc cggcacctgg
     3421 tgtcaagcgc gcgcacgggt tcattggact gccgggtaat caggtcctca agcagccgca
     3481 cgtgttcgtc cggaggcagc gacggcctga tatcaaagca aaacgcgctg ccgccggtgt
     3541 tatccttgtc gatgattttc agcaacaggt cgttccggcc ctgcttgagg cgaatcggaa
     3601 tcgcctcctg ccggggctcg gtgttgcgat agaagaaacg ctcaaagatg atctcgccgt
     3661 tgaggttcag tacgagaccg tcgtcgaagc cgaggtatag gatggcgtcc gtctcagcgg
     3721 gcgagtcgat tcgccggtac agataggccg ccgcgctgtg tctggtttct cgtggcttga
     3781 gcttctctct gatctcgtgg accttgttgt cctgatccca ctcgggcacc tcaatccacg
     3841 caactttccc ggccaggccc tcataggtcg ctgaagtgtc aaagcggttg tccggcggca
     3901 gcggccggtc gccccagaac ctgcggttct ctttcgtcca ttcgaaaagc cccagggcgt
     3961 gccagtgact gctgttgagg cgatagacgg ggtggacgct gtctgcctcg gcctgcccag
     4021 cgcttgctgc cgcagatgag ccgcaccata cggcgagtgg gagcagtacc agactgaaaa
     4081 agcaccctga aaaggagacg cagttctttt cgttggcttt catattccac gcaaccttga
     4141 cgcccatgcc tcgacaagtg cagtcgggca tgctctggac gccgccttgc ttcggccaat
     4201 agcaccgatc c
//
LOCUS       DAOTPW010000365         4072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_17734_length_4072_cov_4.085387, whole genome shotgun sequence.
ACCESSION   DAOTPW010000365 DAOTPW010000000
VERSION     DAOTPW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17734_length_4072_cov_4.085387"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            543..1271
                     /gene="rnc"
                     /locus_tag="P5316_20905"
     CDS             543..1271
                     /gene="rnc"
                     /locus_tag="P5316_20905"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRR87473.1"
                     /translation="MSDSEALQALQEAIGYRFRDPKLLVCALTHASIANTRVNSNERL
                     EFLGDAILGMAVCSYLYRRFPDYLEGEMTKVKSAVVSRRTCAQIARELGLTKCLYMGK
                     GMTSRGQLPSSVAAAVYEALIAAVYIDSGSLDRACEFVLATIEKHLLRATASEHQRNF
                     KSQLQQHAQQVLGATPNYELLDEKGPDHSKCFEVGVVIKGRRYGSAWGPSKKEAEQKA
                     AYLALRELNALEFLPEFEPEGSDR"
     gene            complement(1301..2155)
                     /locus_tag="P5316_20910"
     CDS             complement(1301..2155)
                     /locus_tag="P5316_20910"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HRR87474.1"
                     /translation="MRKSHVLPCMVSNLALAILIGCLVAAGGCTAIIPDKVEASRQHV
                     VRYAAEGEQIKADPVAYLRRLYDRCDALEQYRLTFYRQERVGTVVQTLSPMEQIDALF
                     RKTPFSVKFNWNAPDADYYESVYAEGQNGNKLVIRERNGIFPFPPQVRVIDPALPVKI
                     GKARNAVTDFGLARVTKRTLLPFDDPALAKVMTIRYEGVVDLDPAFRPAHHLRIERPP
                     TPGYAYTRQDFYIDAESLLPAGTDLWLKSGQLGARYRYVDIRTDVQLDDKDFRLTKDH
                     PATIPTQE"
     gene            complement(2283..3305)
                     /gene="aroF"
                     /locus_tag="P5316_20915"
     CDS             complement(2283..3305)
                     /gene="aroF"
                     /locus_tag="P5316_20915"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRR87475.1"
                     /translation="MIVVMQEGCTKEQVNHVVKLVREMKLKDHVIVGTERTVVAVIGD
                     DRFKDRSVLESCPGVDRVVPILAPYKIASRQTHPEPSVIPLGGSMGATVGGKRICVMA
                     GPCSVEGRNQLLEIAHIVKEHGGTVLRGGAFKPRTNPYEFQGLGEKGLEILAEAREQT
                     GLAIVTEVMSVDQVLLVARYADILQIGTRNMHNFNLLVAVGRTNKPVLLKRGWSSTLD
                     EFLLAAEYIIQAGNPNVILCERGIRTHEEYVRNTLTLAMVPAVKRESHLPIIVDPAHG
                     TGRAYMVPSMCRAAVAAGADGLMVEVHQDPEHALTDGAQSLTPDAFAQMMKETRAVAE
                     AVGREL"
     gene            complement(3508..3693)
                     /locus_tag="P5316_20920"
     CDS             complement(3508..3693)
                     /locus_tag="P5316_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87476.1"
                     /translation="MLHTNDQPVGPELPLVTQPHPAACDDVERARVRAHRRCGLLTDQ
                     RSEDGSSRQDDGWYYFV"
     gene            complement(3705..>4072)
                     /locus_tag="P5316_20925"
     CDS             complement(3705..>4072)
                     /locus_tag="P5316_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRR87477.1"
                     /translation="NAEDVLRALELPYRVVNVCTGDLGQGQVQKFDIETWMPSRNGYG
                     ETHSASRFHEFQARRLKLRYRDADRNVRFCHTLNNTVIASPRILIALVENHQTSDGRI
                     RIPKALRPYMGGREIIGGE"
BASE COUNT          776 a         1157 c         1293 g          846 t
ORIGIN      
        1 ccaaagccct tcatgcccgg cttcaactcg tcggtggtca tgtagagcga ccgatccata
       61 tcggccgaaa gaggcccccc caacacggag atggccgaaa cggttaccac cgcaagccag
      121 cttttaacgc tcatcgcccc aagcaataca cgcatccggc gtctccgcac aagagtgttg
      181 aaagcaggca atctatacgt ccgtcgatcg gaccgcaacg gaccgtgatg gagcggccac
      241 cattgcctcg caggtcgtgc tcgcaccacg acggcacaag tctgacgtgt tgacgtcatc
      301 aggacgtgcc gctataggac cgggcggttg tccgcctgtt agtccaactg cttatgtgga
      361 ttaaacttaa cacaggctca aaggtttttc cagttccaat agcttactca atttgacgct
      421 gattccagcc gaggatagga tctgagtggt agtaggaaag tgcctgtggc ctgtcgcgaa
      481 accctcccgg agtagccgtg tggatttcgc cacaagcctg gcacccggtc aggctggaat
      541 ccgtgagcga ctcagaagcc ctgcaagctt tgcaggaagc cattggctat aggtttcgag
      601 atcccaagct gttggtgtgt gcgctgacgc acgcgtcgat cgccaatacg cgagtcaaca
      661 gcaacgagcg gctagagttc ttgggcgacg ccattctggg catggcggtc tgtagctatc
      721 tctacagacg gtttcccgac tatcttgaag gggagatgac caaggtcaaa tcggcggtgg
      781 tctcgcgtcg gacgtgcgcg cagattgccc gggaactggg gctgacgaaa tgcctttaca
      841 tgggcaaggg gatgaccagc aggggacaat tgccgagttc ggtggcggcg gcagtttacg
      901 aagccctgat cgcggcggtg tacattgaca gtggcagtct tgaccgggct tgcgagtttg
      961 tcctggcgac gattgagaaa catctcttgc gggcaactgc ttcggagcat cagcggaact
     1021 tcaagtcgca gcttcagcag catgcgcagc aggttctggg agcaactccg aactacgagc
     1081 tgctggatga gaagggtccc gatcacagca agtgctttga ggtgggcgtg gtgatcaagg
     1141 ggcggcgcta cggcagcgcg tgggggccat ccaagaaaga ggcggagcag aaggccgcat
     1201 atcttgcgct gcgtgagcta aacgcgttgg agtttctgcc cgagtttgaa cccgagggct
     1261 ccgatcgctg atcttgccgc gatgggggga ggctgggcgt ctactcttgc gtcgggattg
     1321 tcgccgggtg gtctttggtc aagcggaaat ccttatcgtc gagttgcaca tctgtccgga
     1381 tgtcgacgta gcggtagcga gcacccagtt gtcccgactt caaccacaaa tcggtgccgg
     1441 cgggtagcag gctttccgca tcgatataga agtcttgccg ggtataggcg tatccggggg
     1501 tcggcgggcg ctcgatgcgg agatgatggg cgggccggaa cgccgggtcg aggtcgacca
     1561 ccccttcgta gcggatggtc atgaccttgg ccaacgccgg atcatcgaac ggaagaagtg
     1621 ttcttttagt gacgcgggca aggccgaaat cggtaacggc gtttcgggct ttgcctatct
     1681 tgaccggcag ggcggggtcg atgacgcgca cctgcggggg gaacggaaag ataccgttcc
     1741 gttcgcgaat gaccagcttg ttgccgttct ggccctcggc atagaccgac tcatagtagt
     1801 cggcgtcagg agcgttccag ttgaacttca cgctgaaggg cgtttttcgg aagagggcat
     1861 cgatctgttc cataggcgaa agcgtctgaa ccacagtacc gacacgttcc tgccggtaga
     1921 aggtcaggcg atattgctca agggcgtcac atcggtcgta gaggcggcgc aggtaagcca
     1981 caggatcggc tttgatttgt tcgccctcgg ccgcgtagcg aacgacgtgt tgccgcgagg
     2041 cctcgacctt atctggtatg atcgcggtgc agccgcctgc ggccacgagg cagccgatca
     2101 gaatcgcaag ggcgaggttt gagaccatgc aggggagtac gtgagatttg cgcacgagtg
     2161 acgatgacct cctgtctggc tgtgacatta taccgtcaga cggtccggtg gagccgctcc
     2221 cgacgttctt ccgccgacag ggatactgca gccctgttgt ctggcggcaa cttgggtgga
     2281 gatcacagct cgcgtccgac ggcttcggcg acggcccggg tctccttcat catctgggca
     2341 aacgcatcgg gcgtgagcga ttgtgccccg tcggtcaggg catgctcggg atcctggtgc
     2401 acttcgacca tgagaccatc agccccggcc gccaccgccg cccggcacat ggacgggacc
     2461 atatacgcac gaccggtgcc gtgggccgga tcaacaatga tcggcaggtg cgactcgcgt
     2521 ttgacggccg gcaccatggc cagcgtcagg gtgttgcgga cgtattcctc gtgagtgcga
     2581 atgcctcgct cgcagaggat gacgttcggg tttcctgcct ggatgatgta ctcggcggcc
     2641 agcagaaact cgtcaagcgt cgagctccaa cctcgtttga gcagtacggg cttgttggtg
     2701 cggccgacgg ccacgagaag gttgaagttg tgcatgttgc gtgtgccgat ctgcaggatg
     2761 tcggcgtagc gggcgacaag caagacctgg tcgacactca tgacctctgt gacaatggct
     2821 aagcccgtct gctcgcgggc ctcggccagg atttcgagcc ccttttcccc aaggccctgg
     2881 aactcgtagg gattggtgcg cggcttgaac gctcctccgc gcagaacggt tcctccgtgc
     2941 tccttgacga tatgagcgat ttccagaagc tggtttcgtc cctcgacgct gcatgggccg
     3001 gccatcacgc agatccgctt gccgccgacc gttgcaccca tcgagccgcc gagtggaatg
     3061 acactgggct ccgggtgcgt ctggcggctg gcgatcttgt atggggccag aatcgggacc
     3121 actcggtcga cgcccgggca gctttcgagg acgctgcggt ccttgaaacg gtcatcacct
     3181 atgacggcaa caaccgttcg ttccgttccg acaatgacgt ggtccttgag tttcatttcg
     3241 cggaccagct tgaccacgtg gttgacctgt tctttggtac aaccctcctg catgacaacg
     3301 atcatgagat tatctccgtt ccgaactgag caggcgcatc gcctgagaaa gtcttctctt
     3361 gtcaaacttg tccatcgggc atcgtccgcc gccggtcgaa gctgtggctc acaccggcgg
     3421 ccgggtaaag ccgaaggtat tgcggccgtg gcgaccgggc cgttggcagc gacgttgacg
     3481 agagtcccgt tgcatgaaga gtctccgcta cacgaagtaa taccaaccgt cgtcctgccg
     3541 gctcgagccg tcctcgctcc gctgatcggt cagtagaccg cacctgcggt gggcgcggac
     3601 gcgggcgcgc tccacgtcat cgcaggctgc agggtggggc tgggtgacaa gtggcaattc
     3661 aggaccgacc ggctgatcat tcgtgtgcaa catggcaatc tcctttattc tcctccaatg
     3721 atctcgcgcc cgcccatata gggtcgcagc gccttaggta ttcggattcg tccgtcggac
     3781 gtctgatggt tctcgaccag cgcgatcaga atgcgcgggc tggcgatgac ggtgttgttc
     3841 aacgtatggc agaaccggac gttcctgtcg gcgtcgcggt atctcagttt gagccggcga
     3901 gcctgaaact cgtggaatcg cgaggccgaa tgcgtctcgc catagccgtt gcgggagggc
     3961 atccatgttt cgatgtcgaa tttctggacc tgtccctggc cgaggtcgcc ggtacacacg
     4021 ttgacgacgc gataaggcag ctccagggcc cgcagcacat cctcggcgtt ct
//
LOCUS       DAOTPW010000366         4024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_17995_length_4024_cov_5.481481, whole genome shotgun sequence.
ACCESSION   DAOTPW010000366 DAOTPW010000000
VERSION     DAOTPW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17995_length_4024_cov_5.481481"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..636
                     /locus_tag="P5316_20930"
     CDS             379..636
                     /locus_tag="P5316_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87478.1"
                     /translation="MGHRNYLTLINGDSWLLPSPVSGLQLTGEDKEDEMRQWHGCSSM
                     MMSLSEGSMMTRTAVLVTGYWTGLFVHRGIGGAYGMLCLGA"
     gene            1078..1809
                     /locus_tag="P5316_20935"
     CDS             1078..1809
                     /locus_tag="P5316_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87479.1"
                     /translation="MEVVPLAPNAKPAIDLLYPEIDTTNPDRFKKLAWPSVIISSGKK
                     VSVAELNWLRESYADITQHPDWRDIASALLWQFELTTQLDELGNRAKPSQASQQDVVA
                     YRARLAETLTVDDTNEYRRIAALVRASSPKSPFAELTLTWTEEGLRHIERGLYGFKVD
                     LPAPSRPVTTATSAGTSEPRKRISAIVLTSRPATQPIATSRADGDFRTSDPEGTSTRI
                     ELTNKIRSTQPAAQPTTKPSDAPTK"
     gene            2721..>4024
                     /locus_tag="P5316_20940"
     CDS             2721..>4024
                     /locus_tag="P5316_20940"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRR87480.1"
                     /translation="MMRRTSRVLATGLLGILAIAVRGAETGVQSGAGIHPSLPADFFP
                     CGAMHMSTISHWQHYLPPVDEWAAYMDKDLARMKEVGLNSMAAHVDWYDIEPAPGEFN
                     FERLDRLMDLVDKHGLRILLWPWPELQPEWVARQYPDGQWLSVTDFKPGPACWDHPAV
                     RKLIDRFVRTVVTRYKDRPSVLAWDVGAEAGVWVSAATPVDRTAAADLYCYCPHTAAR
                     YRDWLRQKYGALDKLNEVWATYYKDWSEIEPVRVGAFERAQIFWLDWRQFMLWNTAEF
                     QRVKAEAARACDPNRPITCHLGGWGWGYVHNSTDEYQIGKHFDFVGLSFFPFWLERGQ
                     AYDSSIGGMMLDGVRSAGDGKPMWVEELQGGPSIFGLSYRSRFPTPSDIRLWNWQCVA
                     HGASGVFYWNWRPETTGIEAAGFGLVNYDGSLTDRARAAGEVA"
BASE COUNT          807 a         1144 c         1299 g          774 t
ORIGIN      
        1 ggattccgcc cgggagagca tccaaatgtc aagagtcaag agttgacccc gtacgcaggt
       61 caagagttga ccccgtacgc agccctggtt cctgtgaacc accctacgag gcagtccgga
      121 attccttgcc gctccttatc aactgtcagc ctgatgtcgg tgccactgtc gggtctacac
      181 gccttcctac ggttttcctg acacccgtcc ccctaagcgt gatacacaaa gaggtgcaac
      241 gggaagtgga gagttacttg tacggcggaa agatctggca gtttgccgcc cacgaagttg
      301 gtcacgggac gtacccgtac cacagcgaag cagccgagca tgccgaagga ggactcatga
      361 tcgagggagg tcccaagtat gggccaccgg aactacctga ctttgatcaa cggcgattcc
      421 tggctcctac cctcgcccgt ttccggtctg caactcactg gtgaggacaa ggaggacgag
      481 atgagacaat ggcacggttg ctccagcatg atgatgagtc tcagcgaggg ttcaatgatg
      541 acacggactg ccgttctcgt gaccggatac tggacagggc tgttcgttca ccgcgggata
      601 ggcggagcct atggaatgct gtgcctggga gcatgataat cgttgttcgg agtctacatc
      661 atgttgatca gtccgtttct acaatccggc ggcagcttga cgcgatacct cgtgacgatg
      721 tcatcacttg cgatcacttt cgattcaaca cgcgctgcgg agacggttga gtggtggctt
      781 gacacgaatg aagtgatggt tggcggaccc gtgtgcgtga cgtttgcccg gttgccgcag
      841 cgtctttgga gcgatgtcga agttgaggtg tcaggccata ggccattctg cccggacctc
      901 cgcggtgatg accgtgcaaa cggccaagtg atttgtcttt tgtggcagtc atctccggag
      961 gaatggatcg aggcgtctcc agacataaca caccttcgtc gtactcctct cttcggggag
     1021 cctggcatct cgacgatttc ggttgtgatc gctggccaac ggtgcgggga agaccgagtg
     1081 gaggtcgtgc ctcttgcccc gaatgctaaa ccggccatcg accttcttta cccggagatc
     1141 gatacgacca acccagaccg attcaagaag ctggcgtggc cgagcgtcat catcagctcc
     1201 ggcaagaagg tttcggtcgc cgagttgaat tggctgaggg agtcctatgc agacataacg
     1261 cagcatcccg attggcgaga cattgcctcc gcgttgctct ggcaattcga actgacaacg
     1321 caactcgatg aacttggcaa tcgtgcaaaa ccgagccaag cctcgcagca ggacgttgtg
     1381 gcctacaggg cccgtcttgc tgagaccttg acggtggacg acacaaacga gtatcgccgg
     1441 atcgctgcac tggtgcgagc aagctcgccg aaaagcccct ttgcggagct gaccctgact
     1501 tggactgaag aaggactccg ccacatcgaa cgaggactct atggttttaa ggtcgacctg
     1561 cccgcaccca gccgtccggt gaccaccgcc acctcggcag ggaccagtga accgcgaaag
     1621 cgaatctctg ccatcgtcct gacgagccgc ccggcaacac aaccaattgc cacatctcgg
     1681 gctgacggcg atttccgcac cagtgatcct gaggggacat caacccggat cgagctgacc
     1741 aacaagatcc gatccacaca accggcagcg caaccgacaa cgaaaccatc cgatgcgccc
     1801 accaagtagg ccgcgccggc ggatacccag gaagaaaacc cgcgaggcgg ccgataccgc
     1861 cgtttatccg cgtccatctg cgccgatctg cggaccggag gtgaggcgat cgcccatagt
     1921 agtcctcaga cgtcggttct ccgttgtcag tcgaaccagg tcgccgagca attagcaatt
     1981 cgggggacac catacctatt tcgatgtttg ttttggtttc cggcctggtt tttggggtgt
     2041 gaggatgcgg ttgagggtgg cttcgagtcg ggtgaggaag gcctggtttc ccagcgggcg
     2101 tccggttcgt tcgtggcgtt ggaagctgtc ccggtttctg gtccttggta agggggagag
     2161 agagacaccc ccttcggctc cccattggta acggggagag aaaacagaga ccgtggtcat
     2221 cgagaccccg cgcggcgttg cgcaagagaa gggaggccgg agaggaaaga ggccggcccg
     2281 atgcactggc agaacaggtg agcaggagag cagaagaaga gagaactcgc cggggcaaga
     2341 atgggccaac cgaccgtgag attgcacggt ttggtccgtc gggaccatgc tcacccaagg
     2401 cgtccggcct caaggctgaa cgttgcgggg ctgtcaacag agcaccctca tgcctcagct
     2461 ttgcatccgt cggggtcttg agcgatcatg ccacctaccg gctgcgccac tcccgtcgat
     2521 gaagccgacc ggctcttgtc agggggggct tcgtggaggt aaggccgcgt gaactgccga
     2581 agaggccctg ggccccgcgt cacgccgcga gcgtgttcgt gtacaaaaac gccctgtgga
     2641 tggttgcggg caacaacatg acatcggatg tgtggaagcg gactggtggc gaatagcgag
     2701 ccggatgtgg gagatacccg atgatgcgaa gaacaagtcg agtattagcc acaggactgc
     2761 tgggaattct ggcgattgcg gtccgagggg cagagacggg cgtgcagagc ggcgccggca
     2821 ttcacccttc gctgcctgcc gacttctttc cctgcggggc gatgcacatg agcaccattt
     2881 cgcactggca gcactatctg ccgccggtgg acgagtgggc ggcgtacatg gacaaagacc
     2941 tggcccggat gaaggaggtc gggctcaatt cgatggccgc tcacgtggac tggtacgaca
     3001 tcgagccggc gcccggcgag ttcaacttcg agcggctgga ccggctgatg gatctggtcg
     3061 acaagcacgg cctgcggatc ctgctgtggc cctggcctga gctacagccg gaatgggtgg
     3121 cgcggcaata ccctgatggc cagtggctgt cggtgaccga cttcaagccc ggcccggcgt
     3181 gctgggacca tcctgcggtt cgaaagctga tcgaccggtt cgtcagaacg gttgtgacgc
     3241 gatacaagga tcggccgtcg gtgctggcgt gggacgttgg ggctgaggcc ggcgtgtggg
     3301 tctcggcggc aacgccggtc gaccgaaccg ccgccgccga tctttactgc tattgcccgc
     3361 atacggcggc gcgataccgc gactggctga ggcagaagta tggcgcgctg gacaagctaa
     3421 acgaggtatg ggccacgtac tacaaggact ggtcggagat tgagccggtg cgtgtgggag
     3481 cgttcgagcg ggcccagatc ttctggctcg actggcggca gtttatgctc tggaacacgg
     3541 cggagtttca gcgagtcaag gccgaggcgg cgcgggcctg tgatcccaac cgcccgatca
     3601 cctgccacct gggcggctgg ggctggggct acgtccacaa ctcgaccgac gagtaccaga
     3661 ttggcaagca tttcgatttc gtgggccttt cgttcttccc gttctggctc gagagaggcc
     3721 aagcctacga ttcgagtatc ggcgggatga tgctcgacgg cgttcgatcc gccggcgacg
     3781 gcaagcccat gtgggtcgag gaactgcaag gcggcccgag tatcttcggg ctgagctacc
     3841 ggtcgcgatt cccgacgcct tctgatatca ggctgtggaa ctggcagtgc gttgctcacg
     3901 gtgcttcggg ggtgttttac tggaactggc ggccggaaac gaccggcatc gaggcggcgg
     3961 gttttggcct ggtcaactac gacggcagcc tcaccgatcg ggcgcgagcg gccggcgaag
     4021 tcgc
//
LOCUS       DAOTPW010000367         3995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_18140_length_3995_cov_5.514467, whole genome shotgun sequence.
ACCESSION   DAOTPW010000367 DAOTPW010000000
VERSION     DAOTPW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18140_length_3995_cov_5.514467"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1557)
                     /locus_tag="P5316_20945"
     CDS             complement(310..1557)
                     /locus_tag="P5316_20945"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRR87481.1"
                     /translation="MVKRFMVIGASMLLATGSVLAAEEATTQPAAAKVVAAGRWNLTV
                     TMTVEKTDPSGQKKTDTWSHPAWLAIEKQGGKHVGRFLGGAGSVHPIGEVKVDGLRVE
                     FNAGDRKWSGVIDNDTISGTWEKGQEKGTWSGKRSVPKTDVAGTWTIRPLGTPIERPA
                     TVELTQADRQITGTYKEDDKKYPIADGVANAGIIRFTVELPSGKKTFGAAVKGDLLDD
                     GMILTQASRLGGSFKGYRQRKWGAPVELFNGKDLSNWEQFGNEKEKNWKVVDGIMTTT
                     GGANIVSKQKFRDFRLHVEFMVPPGGNSGVYLRGRHEIQVADSIGSTPPSWHDCGAIY
                     SRIAPAVNACGKADTWQTFDITMVSNYVTVILNGQIIIDSEEVEGITGGMLDSNEEEP
                     GPIYLQGDHGKISYRKITVWPAE"
     gene            complement(2018..2326)
                     /locus_tag="P5316_20950"
     CDS             complement(2018..2326)
                     /locus_tag="P5316_20950"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRR87482.1"
                     /translation="MNDIPPIKATANPVGLRLPAVPDQAGDEEARQARTTAPVQADRV
                     DISEMGQLLSTLDVGSQIRVDKVTEIREAIANGTYITDAKINYTVSRLMEELKANEEI
                     "
     gene            complement(2628..2927)
                     /locus_tag="P5316_20955"
     CDS             complement(2628..2927)
                     /locus_tag="P5316_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87483.1"
                     /translation="MTKQDIIADIRMRNCSASEEFLATFSEEDLLAYLSNLKEVVPTP
                     VVGQPRSAQDRHAPGRLFGPSRIPFFRSYKQYMVRSTHADTISCIKKSRNRAQLR"
     gene            complement(3341..3553)
                     /locus_tag="P5316_20960"
                     /pseudo
     CDS             complement(3341..3553)
                     /locus_tag="P5316_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009419634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
BASE COUNT          742 a         1236 c         1156 g          861 t
ORIGIN      
        1 tgcgatccag aaacatgcgc gcaaaatgcg caccagttgc gacggaaggg actaagggcg
       61 ggggagaacg agcaggcaaa gcccgaaggc agaggaacca agcataaatc aagccggccg
      121 ctggtcagct tcttttcggg cttgcagcac ggaccgcctc tgccccgcac aattaatgcc
      181 gcaaccgcgc agcacacgag ctgccactat cgacgctgat ccgtcctgcg acagctctcc
      241 cctcccctgc ccgtgcgcta ctcattgggc cgctcttgcg gcttgcagcc gaaaaccggc
      301 acgatccgat cactccgccg gccaaacggt gatcttgcga tagctgatct tgccgtgatc
      361 gccctgcagg tagatcggtc cgggctcttc ctcgttcgag tcaagcatgc cgccggtgat
      421 accctcgact tcttcgctgt cgataatgat ctggccgttg agaatgaccg tgacgtagtt
      481 gctgaccatg gtgatgtcga aggtctgcca agtgtcggcc ttcccgcacg cattgaccgc
      541 cggggcgatg cggctgtaaa tggctccgca atcgtgccaa ctcggcggag tgctgccgat
      601 gctgtcggca acctggatct cgtgccgccc ccgaagatac acgccgctgt tgccgcctgg
      661 cgggaccatg aactccacgt gcaggcggaa gtcgcggaat ttctgcttgg agacaatgtt
      721 ggcgccgccg gtggtggtca tgatgccgtc gacgaccttc cagttcttct ctttctcgtt
      781 tccgaactgc tcccagttgc tcaggtcctt gccgttgaac aactcgacgg gtgcgcccca
      841 tttccgctgg cggtatccct tgaagctgcc acccagcctc gaggcctgcg taagaatcat
      901 accgtcatcg agcaggtctc ccttgacggc ggcaccgaaa gtcttcttcc cggacggaag
      961 ctcgacggtg aaccgaatga tgcccgcatt ggccacgcca tcggcgatcg gatacttctt
     1021 gtcgtcttcc ttgtaggtgc cggtgatctg ccggtccgcc tgggtcagtt cgacggtcgc
     1081 cggccgctcg atgggggtgc ccagcggcct gatcgtccat gtgcccgcca catcggtctt
     1141 cggaacggat ctcttacctg accatgttcc cttctcctgg cccttctccc atgttccgct
     1201 aatggtgtca ttgtcgatca ctccggacca cttgcgatcg ccggcgttga actcgacgcg
     1261 gagaccgtcc actttgacct ccccaatcgg atgaacgctg ccggcgccgc cgaggaatcg
     1321 cccgacgtgc ttgccgccct gcttttcaat agccagccag gcgggatgcg accaggtgtc
     1381 ggtcttcttc tgtcccgacg gatcggtctt ctcgaccgtc atcgtcaccg tgagattcca
     1441 tcgtccagcc gccacaacct tcgccgctgc cggttgcgtt gtcgcctcct cggccgccaa
     1501 caccgagccg gtcgctaaca gcatactcgc cccaatcacc atgaatcgct taaccatgat
     1561 tgcctcccgt tcagcgttgt ccttgttgag tcattgctca attgcaggat tcggcattgt
     1621 ctccgtctag cccaatgttg gcaagcatat ccggccccac ttttcaactt gccgtccgtg
     1681 gtttcgcgag tcggcgaacg ccgacagttc aatcagaccg gaaaagtccg agccggttga
     1741 tcccgatggc ctctcgcaag ccagaggcgc aaacctcgac cggcgcgtca tttcaatcga
     1801 cgcgttttga ggaagggcac cggtgggcgg ccgtttcaga cgcgggggca attgtggacg
     1861 acttcgaacc cccagtgtcg ggcgacaagc ggaaccgtga ttgtggacga tgcgaacggc
     1921 acccaaagca aaagcccacg attgcaggat cgtgggcttg tacgtgagac gttggttttg
     1981 tcggccgaac ccgtgtgttc ggtcgaggtc tagatctcta gatttcctcg ttcgccttga
     2041 gctcctccat gagccggctt actgtgtagt tgatcttcgc gtccgtgatg tacgtgccgt
     2101 tggctatcgc ctcacggatc tccgtcacct tatcaacccg gatttgtgaa ccgacgtcca
     2161 gcgtgctgag caactgtccc atttctgata tatccacgcg gtctgcctgg actggagcgg
     2221 ttgtccgggc ctgccgagcc tcctcatccc ccgcttggtc cggcacggcg gggagcctca
     2281 gcccgaccgg gttggctgtt gccttgattg gcggaatgtc gttcatcggc aatcaccctg
     2341 ttccggtcgc gaaaccttga ggactccacg ctcaaaagca gcgtgaccgg cttgcgcctt
     2401 gcgaatccga ctgcacttgc cacttcgggt tttaggacct cctcacctcc ggcacaggcg
     2461 caagacaacc catgccattg aacttggcat tcctccatga aacgccggtg agtactttaa
     2521 ctgaccccca ccaggattgc gtgaagggcc gatccgtcaa cccgctctca cggtgctctc
     2581 gcaccaatac tgttatcgac gttcctagcg gacgttcttg agaagaatca acgtagctgg
     2641 gcgcgatttc ggctcttttt tatgcaagat attgtatctg cgtgcgttga gcgcaccata
     2701 tattgtttat aggacctgaa aaacgggatg cggctggggc caaaaagcct gccaggagcg
     2761 tgccgatcct gggcggaacg aggctggccg acgaccggag tgggcacaac ctctttcaaa
     2821 ttcgagagat acgccaggag gtcttcctcg ctgaaagtgg cgaggaactc ttccgaggcg
     2881 gagcaattac gcatacgaat gtcggcaatg atgtcctgct tggtcatatg ggtcgctcct
     2941 gaggttcaac tcccaagagg gagaggtgta ttcccgcgtt ttccgatcag gctcctgctg
     3001 gtcgtggatg agtcatcggc cgtcggcccg aaagaacttt cggcgatttg acgggtgttt
     3061 gttgcgcgtc ccggcgcgac ttctgcgcgt cacgagtgct tgcgggcagc acccctatca
     3121 ggcccattta ccggcaccgt cagccgagcg gaagcccgga atctcttctt cgcacagcag
     3181 ggcggtcacg gaacaaccga ccgcctgcgc cgtgcgaagc cgcaccgatg aacgagcctg
     3241 ccggctagac tccaaagccg ccgctttgcg cgtgctgagc atcagtatct tgtctgctgt
     3301 tcaatccctg ggcaggaatc agaggacgtt ctcatgacgc ttcgcatgcg tctccggtgc
     3361 cgtcgccgtc ggcatcagcc tgatcaggat tggagactgc aggacaattg tcgatggaat
     3421 cctcgatgcc gtcatcatca tcatcggagt cacatgcgtc gccagccccg tcgccgtcgg
     3481 tgtccgtctg gccgggattg gccaccgtca tgcaattgtc caaccggttg cggatgccat
     3541 cgttgtccaa atcggtccaa gtgccgggga agccgccggg aggtactgcg gcggcaaccc
     3601 aggccttgaa gacatcctga ataagctgca cgatgccatc cgcaaaagcc tgggcaacct
     3661 cctgcattgc ggatgggccc tgaacctcgg cgttgaccgg gccgggtgga ttaatcatct
     3721 cggcatgccg tcggctgtag taggcaaggg catctcgaat ccagagcccg ggagcccgtt
     3781 cggcaacgga gccggactgg acatggtcaa ggggcgtctc cggtcgagat gtctgagcaa
     3841 aggcgcgtgg aggcgcgaag gtgagcgcta atgccgggag agcaaagacg gccaggccgg
     3901 tgaggaagcc gcgaaaagtc agcatagggt caactctcct ttagaacctt acggccagat
     3961 atctgtgcgg cttgcgcgca tgtttctgga tcgca
//
LOCUS       DAOTPW010000368         3894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_18696_length_3894_cov_5.455066, whole genome shotgun sequence.
ACCESSION   DAOTPW010000368 DAOTPW010000000
VERSION     DAOTPW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18696_length_3894_cov_5.455066"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="P5316_20965"
     CDS             <1..224
                     /locus_tag="P5316_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87484.1"
                     /translation="PSASGSERFLLPWRLEQDYGISRTTLRFVMPSHECIQYEVYFDT
                     KDARQGTVRSFPASRATATGSAWGTEPAR"
     gene            293..760
                     /locus_tag="P5316_20970"
     CDS             293..760
                     /locus_tag="P5316_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87485.1"
                     /translation="MEPFIYCYENVGDNRYVDRGRMTSDGKLFILPHDRSNRSWAVLA
                     FDDWDSDDLNDFIACEFENIVWLNRNVGPGGPGKPPVFATTQGIAILKPWTVQMTSGA
                     QATDWNGDGDLDIVTGQGHGGTGLRFFERDYLNDTLRGTHPRVTVGSAEWRSG"
     gene            829..2157
                     /locus_tag="P5316_20975"
     CDS             829..2157
                     /locus_tag="P5316_20975"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87486.1"
                     /translation="MQRRNEMKRRTFLARAGRAGLGAATAHWMATAVNAQSRPAGANE
                     RIRLAFVGTRGQGRFNLGLFLKEPDVEVPVVCDVDLVEAEKARELTEDKAEVVQDYRR
                     LLERKDIDAFVVCTPDHWHAIITIEACQAGKDVYIEKPMTLCVAEGRRMIEAVRKHNR
                     VVQVGTQQRSGEHYAEAARLVQSGGLGKVFHVFTWYVNNRWPGVGPPPREQPPATLNW
                     DMWLGPRPLVPYDRSRCSGSHRFYWDYAGGQMTDIGTHHMETIHWFMNARAPLSAVAI
                     GQKSMKNEPFDTPDTLNALWEYPGWTLEFAIREANAYTREGSMYGILFHGSEATLYID
                     RAGFELTPEKGKKPVQVVGTPRKQNYLPEALSARHIRNFLDCMRTRQKPNTDVEAGHQ
                     ATTVTHLGNIAYRTGRKIRWDADREQIIDDPEANELLTRTYRPPYLLPKV"
     gene            complement(2410..2973)
                     /locus_tag="P5316_20980"
     CDS             complement(2410..2973)
                     /locus_tag="P5316_20980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR87487.1"
                     /translation="MTNDVWHSSPNSVQITAGPGSIWSSHVGESYTGEIWHRMYVKGS
                     LESTAAGSAWSLEIQGGASDNSMGRWYGGATGIRPRIRGTGRVLNPVALTGGWDLCEV
                     KIMPGTGNDGYSEFFFNGVSLGTLQWGDLDGGQDSQAVDVVRINRWDRSDMSGVACWV
                     DDMSLTPEPTTLVLLGLGGLISLRRRA"
     gene            complement(2990..3124)
                     /locus_tag="P5316_20985"
     CDS             complement(2990..3124)
                     /locus_tag="P5316_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87488.1"
                     /translation="MKALGMCALLVVAASPAMAWIETFDSYVPGGLVRRQRRELVGKR
                     "
BASE COUNT          793 a         1253 c         1150 g          698 t
ORIGIN      
        1 acccgtccgc aagcggcagc gaacgattcc tgctgccgtg gcgactcgag caggattacg
       61 gtatcagtcg gacgacgctg cgttttgtga tgccttctca tgagtgcatc caatacgagg
      121 tgtacttcga cacgaaggat gcccgacagg gaaccgtccg cagtttcccg gcgtcgaggg
      181 cgacggcgac tggttctgcc tggggtacgg agcccgcgag atagccgccg gcaaaacgga
      241 cgctttctgt gacctcgaca acgacggcga cccggacctg ttcaaggcaa ccgtggaacc
      301 gttcatttac tgttacgaga acgtcggcga caatcgctat gtcgatcgcg gccggatgac
      361 atccgacggc aagctattca tcctgccgca cgaccgtagc aaccggagtt gggccgtgct
      421 ggcgttcgat gactgggaca gcgacgatct gaacgacttc atcgcctgcg aatttgaaaa
      481 catcgtgtgg ctcaatcgaa acgtcggtcc gggcggaccg ggaaagccgc cggtcttcgc
      541 gacgacgcag ggaatcgcga tcctgaaacc gtggacggtg cagatgactt caggagccca
      601 ggcgacggat tggaatggag acggcgatct cgatatcgtg accggccagg gacacggggg
      661 cacgggtctg cggttcttcg agcgtgacta ccttaatgac actttgagag gaacacatcc
      721 ccgggtgacc gttggcagcg cggaatggag aagcggttga aggccgccga tctcaaaccg
      781 cacactcgtt gtacaggtca tgcaggacca ggaccatgga ggactgcgat gcagcggaga
      841 aacgaaatga agcggcggac gttccttgcg cgagcgggcc gggcgggtct gggagccgcc
      901 acagcacact ggatggcaac ggccgtcaac gcccaatcac ggccggccgg ggccaacgag
      961 cgaatccgtc tcgccttcgt gggcacccgt ggccaggggc gattcaatct cggactgttt
     1021 ctcaaggagc ccgacgttga ggttcccgtg gtatgcgacg tggacctcgt cgaggccgag
     1081 aaggcccgcg agctgaccga agataaggct gaggtggtgc aggattatcg acgattgctc
     1141 gagcgcaagg acatcgacgc tttcgtcgtc tgcaccccgg accactggca tgccattatc
     1201 accatcgagg cctgccaagc cggcaaggat gtctacatcg aaaagcccat gaccctgtgc
     1261 gtggccgagg gccgacgaat gatcgaagcg gtccgcaagc acaaccgcgt cgtccaggtc
     1321 ggcactcagc aacgcagcgg agaacactat gccgaggccg cccgactcgt ccagagcggc
     1381 ggcttgggta aggttttcca tgtgttcacc tggtacgtga acaatcgttg gcccggcgtc
     1441 ggcccccctc cccgagagca gccgcctgct acgctcaact gggacatgtg gctggggcca
     1501 cgcccgctcg taccgtacga tcgctcccgt tgctctggct cgcaccgctt ttactgggac
     1561 tatgctggcg gccagatgac cgacatcgga actcatcaca tggaaaccat tcactggttc
     1621 atgaacgcca gagcccccct ttctgccgtc gccatcggcc agaagtccat gaagaacgaa
     1681 cccttcgaca cgccggacac gttgaacgct ttgtgggagt atcccggctg gaccctcgag
     1741 ttcgccatcc gcgaagccaa cgcttacacc agggagggca gtatgtacgg gatcctgttt
     1801 cacgggagcg aggccaccct ctacatcgac cgggcgggct ttgaactgac gccggagaaa
     1861 ggaaaaaaac cggtccaggt ggtgggcacg ccgcgcaagc aaaactacct gcccgaagcc
     1921 ctgagtgccc gccacattcg caacttcctc gactgcatgc ggacccgaca aaagcccaac
     1981 accgacgtcg aagccggcca ccaggcgacc acggtcacgc acctgggcaa catcgcgtac
     2041 cgcaccggcc gaaagatccg ctgggacgct gacagggaac agattatcga cgatcccgag
     2101 gccaacgagc tgctgacgag aacctaccgg ccgccctatt tgctgccgaa ggtttagagc
     2161 cccgcgatgc aggccggacg ggcggaaatg agcaatggcg aatgtggaat cgtgaaacgc
     2221 gcgccttgcc ccggcgctcg cggatccttg atcgccatat cagatcgaga cccgggcaac
     2281 ggcgcgcacg cccctccttc cctctattcg cggtcgacaa ggacaacagc ggccgcgacg
     2341 gcccgatccg tcgaagacct tatcgctgct gaatttcagc aggccaattc agaccggtcg
     2401 gtcggacgtt catgctcgac gacgcagcga gatcaatccg cccagaccca gcagaaccag
     2461 cgtcgttggt tctggcgtga gggacatatc atcaacccaa caggcgacgc cggacatgtc
     2521 ggagcggtcc caccgattga tccggaccac gtcgacggcc tgggaatcct ggcctccatc
     2581 caggtcaccc cattgcaggg tgcccagcga gactccattg aagaagaact cgctgtaccc
     2641 gtcgtttccc gtccccggca tgatcttgac ttcgcaaagg tcccacccgc ccgtcaaggc
     2701 gacggggttg agcacacggc cggtgcctct gatacgcggt ctgataccag tagccccgcc
     2761 gtaccatctg cccattgagt tgtcgctcgc tccgccttgg atctcgaggc tccatgcgct
     2821 gcccgctgcc gtactctcca gacttccctt gacatacatc cgatgccaga tctcgccggt
     2881 gtagctttca ccgacgtgac tggaccaaat gctgcccgga cccgcggtga tctgcactga
     2941 gttcggcgac gaatgccata cgtcgttggt cacctcaatc accccggcgt taccgcttcc
     3001 ccaccaattc ccgccgctga cgtcggacaa gcccccccgg cacgtagctg tcaaacgtct
     3061 cgatccacgc catggccggc gaggccgcca ccaccagcaa cgcgcacatt cccaatgctt
     3121 tcatcggatc ctccttcctg ttctgcattc gcacgaacac aatgaacacg cgtttccgcg
     3181 cacgacgggc ccaacgcccg tgtgcgtctg cggcctcata ctgtttcagc acaacccggt
     3241 tgacacctca cggcggccgg cgcgctaacc tcgggataga tagcaggtag ctcggacaca
     3301 cacggatgaa ccgctgctac tacgatgtga aacggggacg ctctctctgc ccctcggtta
     3361 atcccctgct gcgtggtctt ccgttcccga ccatggcccg ccggatcccg ccgatggttc
     3421 tgagtataga tggcgtgtgg gggaattatg aagaaaaaag tacataattg cgacggtgtt
     3481 tttgaatcgc caagtccggt gccaaaccgc ccgaaacgga tcggataagg gcaattcgtg
     3541 gctggccgag gacccggagg cggtggttca ctcggaggca ataccatatc gagagcatcg
     3601 cgagaagatc gctcgaagcg agccgtggaa gatgtaaatg tcgcccacac gtaccgttgg
     3661 tctcttccgt cgcgactggt gcgcattttg cgcgcatgtt tctggatcgc cgggtgccat
     3721 gcccacggct ttgccgggtg ccatgcccac cgctttgccg ggtgccatgc ccacggcttt
     3781 gcgtgggcat gcggaggtag gaccgctcgc cccggaatcc tttccgggcc gcactctcgt
     3841 ccgctcggcc cggaatcctt tccgggccgc atcccctgcg gaatcctttc cgca
//
LOCUS       DAOTPW010000369         3833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_19074_length_3833_cov_5.155373, whole genome shotgun sequence.
ACCESSION   DAOTPW010000369 DAOTPW010000000
VERSION     DAOTPW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19074_length_3833_cov_5.155373"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1565)
                     /gene="hemG"
                     /locus_tag="P5316_20990"
     CDS             complement(54..1565)
                     /gene="hemG"
                     /locus_tag="P5316_20990"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HRR87489.1"
                     /translation="MADTRSDHKRIIVVGGGVSGMAAAYRVAELAAARGTPIDVRVLE
                     ARDQLGGSLVTNRHDGFLVEAGPDSFITQKPWALALCKRLGLTDEIIPTNPAFRRTFV
                     VRKGRLHPIPEGFLLLAPSRIMPFVTSRLFSWPGKLRMGLDLILPAKDHGHDGDESLA
                     SFVRRRFGREALERVAQPLVGGIYTADPEMLGLRATMPRFLEMEAEHRSLILGMRRAA
                     KAMARASKQRNTAQSVDSGARYSMFVSLASGMSRLIEVLRSRLPADAIRTNAPVTRIA
                     PVRNRWQVSLADGTGEIADGVILTCAAYHSAELVRTLDTSLADDLASIDYASSATMSL
                     AYRREDVPGPLDGFGFVVPFVENRTLIAVTFNSVKFAGRAPDGWVLMRAFLGGAMQPE
                     VYEMDDARLRDAVLRDLRDLLGIEAPPRFVELYRWPRSMPQYPVGHLHAVAAIHRKLA
                     TWPGLAIGGSAFGGVGIPDCVHSGETAAEAVVTHVAGVPDSNSAAWPGKSAAS"
     gene            complement(1713..3188)
                     /locus_tag="P5316_20995"
     CDS             complement(1713..3188)
                     /locus_tag="P5316_20995"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRR87490.1"
                     /translation="MLGSFLRVLFDDARRGDDRAGPGLGLNLPSGKPPILRRRTTCLI
                     AVLGIAAILYGTLMPFQIDHSKAISWNLQWTMSMPGDAVANVLIYIPIGAFLRLMLRR
                     RGSPRVIEWGVSLIIAGAISYLTEVAQTVVVARVPSWIDTICNFTGTAIGIAFAPSLQ
                     RILRNAHAWLYRELRVHPMAMAAMTVMICVCTYALAPMDVKPTPTHLAKGIAHLRALP
                     QQWLWLPGDASLSPMAVMDKVISAGAYGLLAFVLLLSAREAGRSRMRSVWYALTRSLA
                     LAVVVEAIQLFTISHVADPRDLFTAWLCCLLGCVVGWTVVGTSPDIHQSPMTLLRGLV
                     AVISGVVLAWAVGSIILSTPAQAPMHTTWWPAVGNFNRSWSSLLGDYTSGLLQYILVA
                     GLIVLWSRATRHRPSMALVVTSTWIIAVTSISVTAFRGYNIDTAQLILGVVGGIIAVR
                     FDRAIFGRQRVLSSNSAGDITPTTPLVPDTAALPSPESRRS"
     gene            complement(3452..>3833)
                     /locus_tag="P5316_21000"
     CDS             complement(3452..>3833)
                     /locus_tag="P5316_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87491.1"
                     /translation="EAGIESDYYVKTFHHDRYWSAIPRERRQEFSVDRERHLDHDMFH
                     DNIFDLFPEQTAEFMAGVDKPFVAFKVLAGGAIPPEDGFRFAFENGADFICVGMFDFQ
                     IVDDVNISLDVLADMSRRTRPWRA"
BASE COUNT          764 a         1181 c         1257 g          631 t
ORIGIN      
        1 tcgggagtca aagacagcgg ggttttcggt ccggttgcct ctgggcgcgg ccgtcaggac
       61 gctgccgact tgccgggcca tgcggccgaa ttcgaatccg gcacacccgc cacgtgcgtt
      121 acgacggcct cggcggccgt ttctcccgag tggacgcagt cgggaatgcc cacgccgccg
      181 aaggcacttc cgccgatagc caggccgggc cacgtcgcca gctttctgtg aatcgctgcc
      241 accgcgtgaa gatgtcccac cgggtactgc ggcatcgacc tgggccagcg gtacagctcg
      301 acgaaccgcg gaggggcttc gataccgaga agatcccgga ggtctcgcag gacggcgtcg
      361 cgcagtctcg catcgtccat ctcatagact tcaggctgca ttgccccgcc aagaaacgcc
      421 cgcatcagca cccagccatc gggagcacgg ccggcgaact tcacgctgtt gaaggtcacc
      481 gcgatcaaag ttctattctc cacgaagggc acgacgaacc cgaaaccatc aagcgggccg
      541 ggcacgtctt cacgtcgata cgccagcgac atggtcgccg acgaggcata atcaattgag
      601 gcgagatcat cggccagact cgtatcgaga gtccggacca gttccgccga atgataggcc
      661 gcgcatgtaa ggatcacgcc atcggcgatc tcgccggtgc cgtcggcgag tgaaacctgc
      721 caccgattcc ggaccggtgc gatgcgggtc acgggtgcgt tcgtccggat cgcatcggca
      781 ggcagacgcg atcgcagcac ttcgatcagc cgcgacatac cggaagccag ggaaacgaac
      841 atgctatatc gtgcaccgct gtcgacgctt tgggcggtgt tgcgttgttt ggacgcccgg
      901 gccatggcct tggccgcgcg ccgcatgccg agtatcagac ttcgatgctc ggcctccatt
      961 tcaagaaagc ggggcatcgt ggctcgcagg ccgagcatct cgggatcagc ggtatagatg
     1021 cccccgacga gcggctgggc gacgcgttcc agggcttcgc ggccgaaacg acggcggacg
     1081 aatgaggcca ggctttcatc gccgtcgtgg ccgtggtctt ttgcgggtaa gatcagatct
     1141 aggcccatgc ggagcttgcc cggccacgag aacagccggc tggtcacaaa gggcatgatc
     1201 cgcgacggtg ccaggagcag aaagccttcg gggatcggat gcaggcggcc cttacggaca
     1261 acgaaggtcc gtcggaaggc tggattggta gggatgatct cgtcggtgag tccgagccgc
     1321 ttgcacagtg ccagggccca gggtttctgg gtgatgaacg agtcggggcc ggcctcgacg
     1381 aggaagccgt cgtgccggtt ggtcaccagc gagccgccga gctgatctct ggcctccagg
     1441 acgcggacgt cgatcggcgt gccccttgcg gcggccagct cagccacccg gtaagcggcc
     1501 gccatgcccg atacgccccc accgacaacg atgattcgtt tgtgatccga tcgcgtatcc
     1561 gccatcacag aacccaggca accaccatgg ctctggaccc ctgagcgcgc aggtgatccc
     1621 tctggtcatc atagtgacgt cggagaagaa tgacaggggg caaacggccg accccgcgca
     1681 ccgtcggcgc tgcctggagg tttcagggca ggtcaggatc gacgcgattc cggagaaggg
     1741 agagcagcag tatccggcac aagcggagtt gtcggcgtga tgtcaccggc cgagttcgag
     1801 ctgagcaccc tctgcctgcc gaagatggcc cggtcgaagc ggacggcaat gatgccgccg
     1861 acgaccccca ggatcagttg ggcggtgtcg atgttgtagc ctcgaaatgc ggtcacgctt
     1921 atggaggtaa ccgcgatgat ccaggtggag gtcaccacaa gggccatgct gggacgatgt
     1981 cgggttgccc ggctccacag cacgatcagc ccggccacca gaatgtattg cagcaggccg
     2041 ctggtgtagt cgccgagcaa actgctccac gagcggttga aattgccgac tgcaggccac
     2101 caggtcgtgt gcatcggggc ctgtgccggg gtgctcagta tgatggagcc gacggcccag
     2161 gccagcacca cgccgctgat caccgcgacc aggccgcgaa gcaaggtcat cggcgactgg
     2221 tgaatatccg gagaagtgcc cacgacggtc cagccgacga cacagcccag caggcaacag
     2281 agccacgccg tgaacaggtc gcgaggatcg gccacgtgcg agatggtgaa caactggatg
     2341 gcttcgacca ccacggccag cgccagggaa cgggtgaggg cgtaccagac ggagcgcatc
     2401 ctggagcgtc cggcctccct cgccgaaagt agcagcacaa aagccagcag gccgtaggca
     2461 ccggccgata tcaccttgtc catgaccgcc atcgggctga gcgatgcatc acccggcagc
     2521 cacagccatt gctgcggcaa cgcccgcaga tgtgcaatac ccttggccag atgcgtcggc
     2581 gtgggcttta cgtccatcgg ggccagggcg taagtacaca cgcaaatcat gaccgtcatc
     2641 gcggccatag ccatcgggtg tactcgcagc tcgcgataga gccacgcgtg ggcgttacgc
     2701 aggatgcgct gaagggatgg ggcgaaggca atgccgatcg ccgttccggt gaaattgcag
     2761 atggtatcga tccagcttgg aacccgggcc accacgacgg tctgggccac ctcggtcagg
     2821 tagctgatcg cgccggcgat gatcagactc acgccccact cgatcacgcg gggcgaaccg
     2881 cggcggcgca gcatcagccg caggaaggcg ccgatcggaa tgtagatgag cacgttggcg
     2941 accgcatcac ccggcatcga catggtccac tgcaggttcc atgagatcgc tttggagtgg
     3001 tcgatctgga agggcatcag cgtgccgtag aggatggccg caattcccag cacagcaatc
     3061 aggcaggttg ttcggcggcg aagtatgggc ggcttgccgg aaggcaggtt cagaccgaga
     3121 cccgggccgg cgcggtcgtc gccgcgacga gcatcgtcga acagcactct gaggaagctg
     3181 cccagcatgg aagccctttc ccgtcacctg gggcgggtac gtaatcccgg atacaaacgt
     3241 agaattcagc ggggcgggaa gcaagcggag caacggaaag agaggcgccg tcccataata
     3301 gcgggattga gcactcacgg caggtttccg ccctgcctgg tccatggtac ttcgcagggc
     3361 ttcgaccgtg gcatagcttg gggagtaaca ccttttcctg cccacaatct gcggcggggg
     3421 ctttcgtcga catggccttg ttccggggcg ctcatgcccg ccatggccgg gtgcgtctgc
     3481 tcatatcggc caggacatcg agggagatgt tcacatcgtc gacgatctgg aagtcaaaca
     3541 tgcccacgca gatgaaatcg gcaccgtttt cgaacgcgaa cctgaagccg tcctcaggcg
     3601 ggatggcgcc gccggccagt accttgaacg cgacaaacgg cttgtcgacg ccggccatga
     3661 actcggccgt ctgctctggg aacaggtcga agatgttgtc gtggaacata tcgtgatcga
     3721 gatgccgctc gcgatcgacc gagaactcct gccgccgttc gcgcgggatg gccgaccagt
     3781 agcggtcgtg gtggaacgtc ttgacgtagt agtctgattc gatgcccgcc tcc
//
LOCUS       DAOTPW010000370         3811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_19208_length_3811_cov_5.572684, whole genome shotgun sequence.
ACCESSION   DAOTPW010000370 DAOTPW010000000
VERSION     DAOTPW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19208_length_3811_cov_5.572684"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1028
                     /locus_tag="P5316_21005"
     CDS             300..1028
                     /locus_tag="P5316_21005"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HRR87492.1"
                     /translation="MDLTLNAIERRVLGVLMEKSLTQPEYYPMTPNAIMAGCNQKNNR
                     DPEMKLDEETVLRTLEALRARNLVTLVLPAPGARTQRYRHEIESAFGWSRRQQAIMTE
                     LLLRGPQTPGELRSRCARMFQFESLEALMTALGSLSKGESDPPLVAAMPREPGQSATR
                     YTHLLYPEDEAPPTAVASPVDHATHPVPHSLSSPAAAPLTDGASAAFRQEIGALQEEI
                     AELHEEMAELRRRLEAVERRTAGL"
     gene            1143..2048
                     /locus_tag="P5316_21010"
     CDS             1143..2048
                     /locus_tag="P5316_21010"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRR87493.1"
                     /translation="MRQSATANGIGIVDALASWARDFEAVIDGMLVPDAAVPRTLAEA
                     VLYSIRSGGKRVRPYVVTRICELCGGTAEMAAPAAVALECVHAFSLVHDDLPAMDDDD
                     LRRGRPTCHKAFGEAEAILAGDALLALAFEISATRIEDPAVVKAVVAELAKGTGWAGM
                     IGGQTTDIQTEGQPPELALVQRIHRYKTARLFVTAGRLGAYCARADEPHLEAAGAYGQ
                     HLGLAFQIADDLLDVTGSAEQMGKAVAKDAEAGKQTYPGAVGIEASQAAARDEARKAI
                     EALQIFGPPAEDLRQLANYVVERRN"
     gene            2103..>3811
                     /gene="dxs"
                     /locus_tag="P5316_21015"
     CDS             2103..>3811
                     /gene="dxs"
                     /locus_tag="P5316_21015"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRR87494.1"
                     /translation="MSLRHDRGGPSEITDNMGLLASIEHPSDVKKLSIAQLNELAKEI
                     RERILEVVGKNGGHLASNLGVAEITIALHYCFDFEKDRLLWDVGHQCYPHKLLTGRNE
                     RFDTLRQSGGISGFPSIDESPFDLFNVGHAGTAIPTALGLARADQLLGRDKRVVAFVG
                     DASIVNGLAFEGLNQAGTLNRQFLVILNDNNYGIAPTQGALAHYLARLRASSIYEEVK
                     QRAKQLLPRLPLVGQSMVDALGHLKEGLKATVSPHSIFEQLGFMYVGPVMGHDIAYLI
                     DLLQMLKDIDHPVLLHIHTNKGQGAEFARAEPSKFHSPTPFIFDGETVRVANGSGRSW
                     TEAFSDAVLEVGNEEPRLCALTAAMPAGTGLDKFAERYPDRFLDVGIAESCTVDVAAG
                     MAKAGLRPLVAIYSTFLQRSFDQVFQEVSLQRLPVILCMDRAGLVGGDGAVHHGFLDI
                     AYLRPLPNLVLLAPADENELKAALRFAVRCPDACAIRYPRADVPPSLGDAAPFELGRS
                     RLMRRGNDATILAYGAMVGNALDAAELLAAEGIELRVFNARFAKPIDREMVTAAMLAD
                     HPV"
BASE COUNT          705 a         1122 c         1275 g          709 t
ORIGIN      
        1 cagcctgttc agaaatatga gaagcggtag gataagtcgg aaaggtccgc tagacgcttg
       61 gaaccttacg accagacatc tgtgcggctt gcgcgcatgt ttctcgatcg cagagcgctt
      121 gggttgcggc ccgcaaaaag gcggtccgcc ttagacctct ggttgtctaa tccttcagtg
      181 aggtctggga cgttcggtgt tcgtcagtct ggggacggtc atgcgtctgg cgtggtctaa
      241 cggtctggaa gtccaagtgc ctagaggtct aaaagcttag caagttggag accgatgcca
      301 tggatttgac cctgaatgcg atcgaacgtc gcgtattggg tgtgctgatg gaaaaatcgc
      361 tgacccagcc tgagtattat ccgatgacgc cgaacgccat catggccggc tgcaatcaga
      421 agaacaatcg tgaccccgag atgaaactgg acgaagagac cgtgctgcgg acgctggagg
      481 ctctgcgggc ccgcaatctg gtcacactgg tgctgcccgc gcctggggca cgcacgcagc
      541 ggtatcgtca cgaaatcgag tctgcgttcg gctggagccg tcggcagcag gcgattatga
      601 ctgagctgct gctccgcggc ccacagacac ccggcgaatt gcgctcgcga tgcgctcgca
      661 tgttccagtt cgagagcctc gaggcgctga tgacggcttt gggctctttg agcaaagggg
      721 agtccgatcc gccactggtg gcggcgatgc cccgtgagcc cggccagtcg gcaacccgct
      781 acacgcacct gctgtatccc gaggacgagg cgccgccgac tgccgtggca tcgccggtcg
      841 atcacgcgac ccacccggtt ccgcattcgt tgtcgagtcc tgcagccgcg cccctgacgg
      901 atggtgcatc agcggccttt cgacaggaga tcggcgccct tcaagaggag atagccgagc
      961 ttcacgagga aatggccgaa ctccgccgcc ggctggaggc ggtcgagcgg agaaccgccg
     1021 ggctgtaggt gtcaatgagg gcgttcattc gcgggccgac aggctggctc ttggggggtg
     1081 tggctatggg ttgcgggctg gtataatctg ccccttgggc ctttggggca gatgaggcgg
     1141 ctatgcggca gtcagcaaca gcgaacggga tcggaatcgt cgacgccttg gcgtcatggg
     1201 cccgggattt cgaggcggtg atcgacggca tgctggtgcc ggacgcagcg gtcccgcgca
     1261 cgctggcgga ggccgtcctg tattcgatcc gcagcggtgg caagcgggtc cggccctatg
     1321 ttgtaactcg gatctgcgag ctttgtgggg gcacggccga gatggctgcc cccgcggcgg
     1381 tagccctgga atgcgtgcat gccttctccc tggtgcacga cgaccttccg gcgatggacg
     1441 atgatgattt gcggcggggc cgtccgacgt gccacaaggc attcggggag gcggaagcga
     1501 ttctggcggg cgacgcgctg ctggcattgg ccttcgagat ctccgcgacc cggatcgagg
     1561 atccggcggt agtgaaggcg gtcgtcgccg agttggccaa gggcaccggc tgggcaggga
     1621 tgatcggcgg acaaaccacg gatatccaga cggagggaca acccccggaa ctggccttgg
     1681 tccagcgaat ccaccgatac aagacagcgc ggcttttcgt gactgccggc cggctggggg
     1741 cgtattgtgc ccgggcggac gaaccgcact tggaggcggc gggcgcgtat gggcaacatc
     1801 tggggctggc gttccagatt gccgacgacc tgctggacgt caccggatcg gccgagcaaa
     1861 tgggcaaggc ggtggccaag gatgccgagg ccggcaagca aacctatccc ggtgccgtcg
     1921 gcattgaggc cagtcaggcc gccgctcggg acgaggcacg aaaggcaatc gaggcgctgc
     1981 agattttcgg acctccggcc gaagatctca ggcaactggc aaactatgtc gtcgagcggc
     2041 gcaactgaat cgcacgaccg actgaatcgg ggcaaacaac ccaagcgacg cgacgtacta
     2101 ccttgagtct cagacacgat cgcggggggc caagcgagat tacggacaac atgggactac
     2161 tggcttcgat agagcatccg tcagacgtca agaagctgtc gatagcccaa ctcaacgagt
     2221 tggctaaaga gatccgggag cgcatcctcg aagtggtcgg caagaacggc ggacacctgg
     2281 ccagcaatct gggcgtcgcc gagatcacca tcgctcttca ctactgcttt gactttgaga
     2341 aagaccgatt gctgtgggac gtcgggcacc aatgttaccc gcacaaattg ctcacgggcc
     2401 gcaacgaacg ttttgacacg ttgcggcaat ccggaggcat cagcggtttt ccgagcatcg
     2461 atgaaagccc cttcgatctg ttcaacgtcg ggcacgcggg cacggcaatc ccgacggcgc
     2521 tgggtttggc ccgcgccgac cagctcctcg gccgcgacaa gcgggtcgta gccttcgtag
     2581 gcgatgcgag catcgtgaac ggcctggcgt ttgagggcct caaccaggcg ggcacgctca
     2641 atcggcagtt tcttgtcatc ttgaacgaca acaactacgg catcgctccg acgcagggcg
     2701 cattggctca ttacctcgct cgcctccgtg ccagttccat ttatgaggag gtcaagcagc
     2761 gggccaagca actgctgccc agattgccgc tggtcggcca gtcgatggtc gacgccctcg
     2821 ggcatctgaa ggaggggctg aaggccacgg tttcgccgca ttcgattttc gagcagctcg
     2881 gcttcatgta tgtcgggccg gtgatgggtc acgacatcgc gtacctgatt gacctgctgc
     2941 agatgctcaa ggatatcgat cacccggtgc ttctgcacat tcataccaac aaaggtcagg
     3001 gcgccgagtt cgcccgtgcc gagccttcga agttccactc gcccacgccg ttcatcttcg
     3061 atggcgaaac cgtaagggtc gcaaacggct cgggcagaag ctggaccgag gctttctcgg
     3121 acgcggtcct tgaagtcggc aatgaagagc cgaggctctg cgccttgacg gcggcgatgc
     3181 cggcgggcac cggcctggac aagttcgcgg agcggtaccc ggaccgcttt ctcgacgtgg
     3241 gaatcgccga gagctgcacc gttgacgttg ccgccgggat ggccaaggcg gggttaaggc
     3301 cgctagtggc tatttactcg accttcctcc agcgatcttt cgatcaggtc tttcaggagg
     3361 tctcgctgca gcgcctgccg gtaatcctgt gcatggaccg ggcggggctg gtcggcggcg
     3421 acggggcggt ccaccacggg ttcctggata ttgcgtacct gcggccgttg ccgaatttgg
     3481 tgttgctggc tccggccgat gagaacgagc tgaaggccgc gctgcgattc gccgtgcgtt
     3541 gcccggacgc ctgcgccatc cgttatccgc gggcggatgt tcctccatcg ttgggtgacg
     3601 ctgcgccttt cgagctgggg cgatcccgac tgatgcgccg aggtaacgac gcgaccattc
     3661 tggcttatgg ggccatggtc ggcaacgcgc tggacgccgc cgaactgctg gcggccgagg
     3721 ggatcgaact gcgggtgttc aacgcccgtt tcgccaagcc gatcgaccgc gaaatggtca
     3781 ccgcggcgat gctggccgac cacccggtca t
//
LOCUS       DAOTPW010000371         3792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_19296_length_3792_cov_5.287931, whole genome shotgun sequence.
ACCESSION   DAOTPW010000371 DAOTPW010000000
VERSION     DAOTPW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19296_length_3792_cov_5.287931"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="P5316_21020"
     CDS             complement(<1..141)
                     /locus_tag="P5316_21020"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR87495.1"
                     /translation="MPRRSTVRHSGRLCGWGKTHAAIALGLAACHAGYRARFATAIDI
                     INN"
     gene            complement(331..2445)
                     /locus_tag="P5316_21025"
     CDS             complement(331..2445)
                     /locus_tag="P5316_21025"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRR87496.1"
                     /translation="MIVRITFVATVAVGLATPVSFGDERLPEAKPVPEVMVIPLPYAQ
                     ASFQHHGRELTRYHFGPTLNRPFLFPLVGPAGCSYAGMAPPQDPIGHSHHNSVWISHQ
                     NVNGVNFWVDGNARIVCQGVSRYEDGPEQAALLSMNAWQDADGKTLMIEQRRIAVEPL
                     GDKEWLMFIDIRLDAPPNSEVTLNTSPFGLIGVRMVKTISVREGGGRILNSQGLRNEK
                     EVFRKPARWVDYSGPVTADATGGITLMDHPANPNHPVPFHVRDDGWMGASLTLTKPLN
                     VRPEAPLRLRYGLWVHAGVPEPERIDPLWKRFAEAPLPEMGPPSNSKTASTAPRIVQT
                     SKPASGPDDLRTIGGEPITVFRDPARYTSFPDVERLPDGRLLCVFRDATYPEQIRHIE
                     ADARIVGSISDDGGRTWSKPFVIYDDPDCQNDPSVAALRDGRLLLSFFNWVGRSEAYV
                     KQHNPPYARRVDRGAWGDYAEPGGVKTLWGKAASLTWELKPRDMGVKAEQLLATSSSA
                     IETSKGTLLLPVYGRSKSQPVDQAYVFRSADGGKTWSEQILMAVDPGGKIAMQEPALV
                     ENADGTIVAVMRTANAQDHMFTARSGDDGLTWSPAERLPLIGHPADLVRLPDKRVLLV
                     YGYRHQPFGVRACVSDDGGKTWDVHRELVIAASGVNTDLGYPSVCLADDSHVVVVYYM
                     NGPNTRDRWIECKRIAIEELGG"
     gene            complement(2552..2899)
                     /locus_tag="P5316_21030"
     CDS             complement(2552..2899)
                     /locus_tag="P5316_21030"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HRR87497.1"
                     /translation="MLYSTFVTLTAPCCTGCGGSSQTKAEMASLSSVLALYQLHLGAY
                     PTSLDDLVVRPSGEAGARWKGPYVENPGTLRDTWGRPLRYKSPGVKNLGHYDLWSAGP
                     DGVDGTADDINNW"
     gene            complement(3050..3526)
                     /locus_tag="P5316_21035"
     CDS             complement(3050..3526)
                     /locus_tag="P5316_21035"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HRR87498.1"
                     /translation="MEPPEREELFTMNYGPEPSPRRCSTREKTFLLLIVAALLLFAVM
                     FLRPRLNGGPDASVRVTRVCLDTVADSLQSYHRSVGSYPTSLADLCRLPPGMSQEVWR
                     GPYVDSVHTLRDGWGQELLYKNPGIHNPQHYDLWSAGKDGEDGTDDDICNWVLDDP"
BASE COUNT          680 a         1133 c         1213 g          766 t
ORIGIN      
        1 gttattgatg atgtcgatgg cggtggcgaa gcgggcgcga tagcccgcgt ggcaggcggc
       61 caggcccaag gcgatcgcgg cgtgcgtttt gccccacccg cacaacctgc ctgagtgtct
      121 caccgtcgag cgcctcggca ttcgcccggc gacgattggc gccccgaagg gagaactcat
      181 ccaccgagct gatccgcaag ctacaagaga atggctcgtg tgagctttta gtctcttcca
      241 tcgggactgg tgcgcatttt gcgcgcatgt ttctggatcg cggtacgctt gggttgcggc
      301 ccgcaaaaag gcggtccgcc ttagcggctt ctagcctccc aattcctcaa tcgcaattcg
      361 cttgcactcg atccagcggt ctcgggtgtt cgggccgttc atgtagtaga cgaccacgac
      421 atggctgtcg tcggccaggc agacgctggg gtagccgaga tcggtgttga caccggaggc
      481 ggcgatcacc agttcccggt gcacgtccca cgtcttaccg ccatcatcgc tgacgcatgc
      541 tcgcacgccg aagggctgat gccgatagcc atagaccagg agaacgcgtt tgtcgggcag
      601 ccgtaccagg tcggccgggt gcccgatcag tggcaaccgt tcggccggcg accaggtgag
      661 accgtcatca ccggatcgag cggtgaacat gtggtcctgg gcgttggcgg tccgcatcac
      721 cgccacgatg gtgccgtcgg cgttttcgac cagggccggt tcctgcatgg cgatcttgcc
      781 accggggtcg acggccataa ggatctgctc actccacgtt ttgccgccgt cggcactgcg
      841 aaaaacatag gcctggtcga cgggctgcga tttcgatcgc ccgtaaaccg gcagcagcag
      901 cgtgcctttc gatgtttcga tggccgacga cgacgtggcg agcaactgtt cggccttaac
      961 tcccatgtcc ctgggcttca gttcccaagt gagcgaagcg gccttgcccc agagcgtttt
     1021 caccccgccg ggttcggcat agtcgcccca cgctccgcgg tcgactcgcc gggcgtacgg
     1081 gggattatgc tgcttgacgt aggcctcgct gcggccgacc caattgaaga agctcagcag
     1141 caaacggccg tcgcgaagcg ccgcaaccga tgggtcgttc tggcagtccg ggtcgtcata
     1201 gatgacaaat ggcttggacc acgtgcggcc gccatcatcc gaaatcgagc cgacgatgcg
     1261 ggcatccgct tcgatgtggc ggatctgctc tggataggtc gcgtcgcgaa agacgcacaa
     1321 gagacgcccg tcgggcagcc gctcgacatc cgggaaggac gtgtatcgcg ccgggtcgcg
     1381 gaacaccgtg atcggttctc cgccgatcgt ccggagatcg tctggtcctg aggcgggctt
     1441 gctcgtctga acgattcttg gtgctgttga cgcggtcttg ctattgctcg gcggtcccat
     1501 ctcgggaagt ggcgcttcgg cgaaccgctt ccacagcgga tcgatacgct cgggttccgg
     1561 tacgcctgcg tggacccata aaccataacg cagccgcaga ggcgcttccg gccggacatt
     1621 cagcggcttg gtcaatgtca ggctggcacc catccagccg tcgtcgcgca cgtggaaggg
     1681 gaccggatga tttgggttcg ccgggtggtc catcagggtg atgccacctg tggcgtcggc
     1741 cgtgaccggt ccgctgtagt cgacccaccg ggcgggtttg cgaaagacct ccttctcgtt
     1801 tcgcagcccc tgcgagttga gaatccgccc gcccccctca cgaacactga tggtcttgac
     1861 catgcgcacg ccgatcaggc cgaacggact ggtattgagc gttacctcgc tgtttggggg
     1921 agcatcgagc cggatgtcga tgaacatcag ccactccttg tcgccgagcg gctcgaccgc
     1981 gatgcggcgc tgctcgatca tcagtgtttt gccgtcagcg tcctgccacg cattcattga
     2041 gagcaaggct gcctgctcag gaccgtcctc gtatcgagag acaccctggc agacgatccg
     2101 ggcgttacca tctacccaga aattgacgcc gttgacgttc tgatgggaaa tccagacgga
     2161 gttgtggtgg ctgtggccga tggggtcctg agggggggcc atgccggcgt agctgcaccc
     2221 ggccgggccg accagcggga acaggaacgg ccggttgagc gtcgggccga agtggtagcg
     2281 ggtcagttca cggccgtgat gctgaaacga tgcctgagca tacggcaggg ggattaccat
     2341 gacttcagga accggcttgg cttccggcaa ccgctcgtcg ccgaatgaaa cgggtgttgc
     2401 caggccaact gccacggttg cgacaaacgt gattctcacg atcattcggc gtcctcctgt
     2461 gaatgggggt cccagtataa cattgggggc gggttgttgc ctgtgatcgg cctctcgtca
     2521 ggcagagcac ttgtccgtga cgatatgtgt ctcaccagtt gttgatatcg tctgctgtgc
     2581 cgtcgacgcc gtccgggccg gcgctccaga ggtcatagtg cccgaggttc ttgacgccag
     2641 gcgacttgta gcggagcggt cggccccagg tgtctctgag tgtgcccggg ttttcaacgt
     2701 agggcccttt ccacctcgcc cctgcttccc cggaaggccg caccacaagg tcatcgagcg
     2761 aggtcggata ggcgccgagg tgcaattggt acagtgcaag cacgctactg agtgaggcca
     2821 tctcggcttt ggtttggcta ctgcctccgc acccagtgca acaaggggcg gtcaaagtca
     2881 cgaatgtcga atatagcatg ggacagagcg tgatcgaggc cagcgtgacg aggattatca
     2941 gggcggagtt tggcggcact gtccttcgag ccgaagcgta gttcagagtc acgggttcag
     3001 tgtgcggatc gtgttccatg gctcgattcc ctatcccgtt ccatgcggat catggatcgt
     3061 ctaacaccca gttgcagatg tcgtcgtcgg tcccgtcctc gccgtctttg ccggcgctcc
     3121 agaggtcata gtgctgggga ttgtgaattc cggggttctt gtacagcaac tcttgtcccc
     3181 agccatccct aagcgtgtgg acgctgtcca cgtaaggccc gcgccagact tcttgtgaca
     3241 tccctggggg caagcggcac aggtcagcga gcgaagtcgg gtatgagcct acgctgcggt
     3301 ggtacgactg gagagagtcc gccactgtgt ccagacaaac gcgggtgacg cgaacggacg
     3361 cgtcaggacc tccgttcagc cgtggcctca ggaacatcac tgcgaagaga agtaacgcag
     3421 caacgatcag caggaggaac gtcttctctc gcgtgctgca tcgccgggga ctcggctctg
     3481 ggccgtagtt catcgtaaac agttcttctc gttcgggtgg ctccatcgcg cacttccgcc
     3541 tctagactga ggtgctctca gggaagtccc gaccagcggc ccaccgattc caagagcttg
     3601 tacaaccaag atcacgatcg gatgcacgcg cgggatagat ggcccaggtc cgaaagcacc
     3661 tgggctgctt gggagaagtg ccgacgaggg cgtcggcact tgtctggacc ggctctgcca
     3721 atagctcaca gttgagagct aacccaagtt gaacagccaa aggcgattcc aggttttttg
     3781 gggggtgtcg aa
//
LOCUS       DAOTPW010000372         3680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_19967_length_3680_cov_5.011034, whole genome shotgun sequence.
ACCESSION   DAOTPW010000372 DAOTPW010000000
VERSION     DAOTPW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19967_length_3680_cov_5.011034"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="P5316_21040"
     CDS             complement(<1..1013)
                     /locus_tag="P5316_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87499.1"
                     /translation="MNHRLISTLVSVACAAPVMGASWPMKHRDPAHTGRADYTVPAER
                     MNNTFFDTVRWQKRSPGSPGEGGFTSSSMVFYDGVGPGGADIVVAGYHWPKGVQGMDR
                     RTGRLFWNGNPSGGETIGASTPAFSNNGGTVYVVNDATANAQYPNGHPLMAFRTNVGP
                     SSYWHNGSDANPGHLSMHSPTIAPDGRIFLHSWVDRPYAGMDDGSMIEQNWAAATPAD
                     CGLSDPSLYTGVSPLMVVIGARWGQIKAYSGSTGAELWTANVGATVDATVTIDPANGN
                     IYVGCGSDSIFVAGLDKNGNPLWGSEAVMVFYNGGASPQHAQAAGCLSHYGLTYYIQT
                     NGQ"
     gene            complement(1060..3387)
                     /locus_tag="P5316_21045"
     CDS             complement(1060..3387)
                     /locus_tag="P5316_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87500.1"
                     /translation="MKQVCRRAALIVGLVWVPNLAAAQDLTVLRPRALLINFDPIIES
                     RSGKRLHTVGGWNDPSALTAGYINDFEVVSHGLLKYRLTRTVTVDVWPIKEDGFRYTD
                     ESYLQCLATWSGWHTPDLCDYKAKVRDFDLARKVDSGEIDEVLDHSAPYFGGYETRMI
                     GRGGYWCNSPALTRVACSRIFIISVFNYERGVGEMLEDFGHRSESILTHVYGGWSAQQ
                     THAWNRFTLYDKVYPGEAACGNVHFAPNSDSDYDWGNWRYVDSTADYWLNVFPEGYPS
                     RISDYKRSMNCSEWGYGDIRLHHKWWFARFPHAAGSITEYGMTRLNNWWAYVQDFNTY
                     PESGGDHVPGGTPPAAEPYPGVCVAVSANLRDDWAPQINAAGRLVWHGSDGSDFEIYS
                     ADRDGRMFTRITNNAFNDEDPKINASGRIVWQGFDGQDYEIYSANADGTDVVRITNNT
                     VNDWHPQINDLGKVVWDSFDGTDYEIYSADADGTNVVRITDNTAASGYPREDVWPQIN
                     NSNRVVWFGYDGSDWEIYSANADGSGLINVSNNGYEDEYPQINDSGRVVWHSWLNSWN
                     TEIYSADATGGPVVRLTNNSYEDWYPQISDTGQVVWMARTATSWEIFQANATGGGLMR
                     ITNNGTHDQYPRINLAGEIVWQGFDGSDWEIYTLREGAILQVTNNNYDDRAPCINDQS
                     QIAWHADSAENTSEIFSAIPFVPVPADFDGDGDVDQEDFGHFQICMTGSGGAITLGCE
                     DAILDGDTDVDGADLDIFMNCLSGADVPGDTGCAG"
BASE COUNT          658 a         1109 c         1162 g          751 t
ORIGIN      
        1 tgttgaccgt tggtctggat gtagtaggta agaccgtagt ggctgaggca accggcggcc
       61 tgggcatgtt ggggacttgc cccgccgttg tagaaaacca tcactgcctc gctgccccac
      121 agcggattgc cattcttgtc caggccggcc acgaagatgg aatcactgcc acagccgacg
      181 tagatgttgc cgttggccgg gtcaatggtc acggtggcat cgaccgttgc gccgacgttg
      241 gcggtccaca gctcggcacc ggtggaaccg ctgtatgcct tgatctgccc ccatcgggcg
      301 ccgatgacga ccatcaacgg gctgacgccg gtatagagag acggatcgct cagtccgcag
      361 tcggcgggag tcgcggcggc ccaattctgt tcgatcatgc tgccgtcgtc catgccggcg
      421 tagggccgat cgacccaaga atgcaggaag atgcggccgt cgggtgcaat cgtgggtgag
      481 tgcatgctga gatggccggg gttggcatcc gacccattgt gccagtagga cgaaggcccg
      541 acgttggttc ggaacgccat cagcgggtgg ccgttgggat actgagcgtt tgccgtcgcg
      601 tcgttgacga cgtagaccgt gccgccattg ttggagaagg ccggggtgct cgcgccgatg
      661 gtctctccgc cgctcgggtt tccgttccag aaaagacgac cggtacggcg gtccatgccc
      721 tggacccctt tgggccaatg atagccggca acgacgatat cggcgccgcc cgggccgacg
      781 ccgtcgtaaa acaccattga gctgcttgtg aaaccgccct cgccgggcga accgggcgag
      841 cgtttctgcc agcgcaccgt gtcgaagaac gtgttgttca tccgctccgc ggggacggtg
      901 tagtccgccc gtccggtgtg cgccgggtcc cgatgcttca ttggccagga agcgcccatc
      961 acgggcgcgg cacaggcaac cgagacaagt gttgaaatca atctgtggtt catcgctcga
     1021 tcctcttcag ttcgaagctc cgccggaaga cggcgggctt tatccggcgc acccggtgtc
     1081 gcccggcacg tcggcgccgc tgaggcaatt catgaagatg tccaagtcgg caccatccac
     1141 gtcggtgtcg ccgtcgagaa tggcatcctc acaaccgagg gtgattgctc cgcccgaacc
     1201 ggtcatgcat atctggaagt ggccgaagtc ctcctggtcc acatcgccat cgccgtcaaa
     1261 gtcggccggg accggcacga acggaatggc ggagaagatt tcgcttgtgt tctcagcgct
     1321 gtcggcatgc caggcaatct ggctctgatc gtttatgcag ggggctcgat cgtcgtagtt
     1381 gttgttcgtg acctggagga tggctccttc gcgaagggta tagatttccc agtccgagcc
     1441 gtcgaaacct tgccagacaa tctccccagc caggttgatt ctggggtact ggtcgtgggt
     1501 gccgttgttg gtgatccgca taaggccgcc gccggtggca ttggcctgga aaatctccca
     1561 actcgtcgcg gtccgggcca tccacacaac ctggccggtg tcgctgattt gcgggtacca
     1621 gtcctcgtag gagttgttgg tcagccggac aacggggccg ccggtggcgt ccgcactgta
     1681 gatctccgta ttccaggaat tgagccaaga atgccagacc actcgcccgc tgtcattgat
     1741 ttgcgggtac tcgtcctcgt aaccgttgtt cgagacgttg atcaggccgc tgccgtcggc
     1801 gttggcgctg tagatttccc aatcggaccc gtcgtagccg aaccagacga cccggtttga
     1861 gttattgatc tgcggccaga catcctcgcg cgggtagccg gaagccgccg tgttgtcggt
     1921 gatccgcacg acgttcgtcc cgtcggcatc ggcgctgtag atctcgtaat cggttccgtc
     1981 aaacgagtcc cagaccacct tgcccaggtc gttgatctgg ggatgccagt cgttcacggt
     2041 attgttggta atccggacaa cgtcagtccc gtcagcgttg gcgctgtaga tctcgtagtc
     2101 ctgaccgtcg aatccctgcc agacgatgcg gccggaggcg ttgatcttcg ggtcctcatc
     2161 gttgaatgcg ttattggtga tccgcgtgaa catccggccg tcgcgatcgg cggagtagat
     2221 ctcgaagtcc gacccgtcgg aaccgtgcca gacgagccgg ccggccgcgt tgatctgcgg
     2281 tgcccagtca tcacggaggt tggcgctcac cgcgacgcag acgccgggat acggctcggc
     2341 cgcgggaggc gtaccgccgg gcacatggtc gcccccgctc tcggggtaag tgttgaaatc
     2401 ctgcacgtag gcccaccagt tgttcaggcg ggtcatgccg tactcggtga tggagccggc
     2461 agcgtgcgga aaacgggcga accaccactt gtggtgcaag cggatgtcgc cgtagcccca
     2521 ctcactgcaa ttcatcgatc gcttatagtc tgagatccgg ctcggatagc cttcggggaa
     2581 aacgttgagc cagtaatcgg cggtgctgtc cacgtaacgc cagttgcccc agtcgtaatc
     2641 ggaatcgctg ttcggggcaa agtgcacgtt gccgcaagcc gcctcccccg ggtaaacctt
     2701 gtcgtaaaga gtgaaacggt tccaggcatg cgtttgctgg gccgaccaac cgccatacac
     2761 gtgggtcagg atggattcgc tgcgatgccc gaagtcctcg agcatttcgc cgacgccccg
     2821 ctcgtagttg aacaccgaga tgatgaagat cctggagcag gccacgcggg tcaaggctgg
     2881 cgagttacac caatagcctc cgcggccgat cattcgcgtc tcatagccgc cgaagtacgg
     2941 ggcgctgtga tcgagcacct cgtcgatctc cccggaatcc accttacggg ccaagtcaaa
     3001 atcgcggacc ttggccttgt agtcacacag atccggcgta tgccagcccg accaggtggc
     3061 cagacactgg agataggatt cgtccgtata acgaaacccg tcctccttga tgggccagac
     3121 gtcgacggtc accgtgcggg tcagacgata cttgagcagt ccgtggctga cgacctcgaa
     3181 gtcgttgatg tagccggccg tcagggccga agggtcgttc catccgccca ccgtgtgcaa
     3241 tcgtttgccg gaccggctct cgatgatggg gtcgaagttg atgagcaggg cgcgggggcg
     3301 caggaccgta aggtcttgag ccgccgccag attcggtacc cagaccaggc ccacgatcag
     3361 ggcggcgcga cgacatacct gcttcatctc cgcactcctt tgagttcgat ggctgctttg
     3421 cttgagaccg ctaccggcca gtccctctcg cgtttattcc agcatatccg gtttgcctcc
     3481 tcatctcaag gcatgagcgg cccaggcggg tcctgataaa agcggtgcgc agggcggact
     3541 ccggctatcg gccgcagcga accgcgatcg cgggccgttc ttacaggggg catctacctg
     3601 ttgtcctgtt cggcgggttc catccgaaac tggagttgtg cgacaccgtc gatttcgaag
     3661 tgctcgacac ggatcgtgtg
//
LOCUS       DAOTPW010000373         3609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_20458_length_3609_cov_5.906303, whole genome shotgun sequence.
ACCESSION   DAOTPW010000373 DAOTPW010000000
VERSION     DAOTPW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20458_length_3609_cov_5.906303"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2384)
                     /gene="glgP"
                     /locus_tag="P5316_21050"
     CDS             complement(<1..2384)
                     /gene="glgP"
                     /locus_tag="P5316_21050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRR87501.1"
                     /translation="MPHVRSFMVLPALPEALKPLRDLAYNVWWTWNPDAIELFRRLDV
                     DLWRELKHNPVAMLAQLRQERLDRLARDPAYIAALRRVQDQLAAYLERPTWFSETYGH
                     EDIGKIAYFSAEFGLHECLPIYAGGLGILSGDHLKSASDLGLPLYGVGLLYRQGYFHQ
                     YLTNDGYQFEDYPELDFATMPVTPVLDASGNQLCVQLDVGGRNVWIKVWKVTVGRVIL
                     YLLDSNHPNNRREDRDITARLYGGDQEVRIRQEIVLGIGGMRALAALGITPSVCHMNE
                     GHSAFLAVERIHQRMREDKLTFEQAREVVSTGTVFTTHTPVPAGIDHFPPALVTKYLT
                     PYCQILGITEQTLLQLGRMQPEDTHEPFSMAVLALRLSYLSNGVSQLHGEVSRKMFGP
                     VYPGVPTNEVPITAITNGVHIRSWLSRDMAHLLDQYLGPQWAEDPLNQAIWQRVDLVP
                     DAELWRTHERLRERLVGFARRRLKAQLIARGAPPAEVDAVEEMLDPEALTLGFARRFA
                     TYKRATLLIYNQDRLRKLLTDPQRPVQVIIAGKAHPKDEAGKELIKRIVAFTKDPRVR
                     NRIIFLEDYDMSVARLLIEGVDVWLNTPTKLHEASGTSGMKVTPNGGINMSVLDGWWP
                     EAYDGTNGWAIGDRRIYNNQDYQDFMEAESLYELLEKELIPMFYDRGVDGLPRRWTAM
                     MKASMRTCCPVFNTNRMVREYTERFYLPAARRWHRFTQDNYKIARDIAGWRRRVTDKW
                     SEVRIESVDAKVPAEVTVGTELPVLAQVRLGSIKPEEVSVELYYGPLDASGQIST"
     gene            complement(2932..3507)
                     /locus_tag="P5316_21055"
     CDS             complement(2932..3507)
                     /locus_tag="P5316_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87502.1"
                     /translation="MLGPLIVRTGDRLFTSATSRLHRLGLRPFTANVLEVAMGILAAS
                     LFASGMRLAGLVALILHGGFDYLDGALARAGAAGSSITRHALTDKLSEAIIFTGPAFG
                     GLIPFWLSLAVIAAAFGATWLGLHGPKKTARREQAVFDRSDRLLSLILLGLFADLSLG
                     AWTNLAMALAVCGQRLRARPPAVPTQARQAA"
BASE COUNT          658 a         1046 c         1170 g          735 t
ORIGIN      
        1 gtgctgatct gtccgctggc atccagcggc ccgtaataca attcgacgga gacttcctcg
       61 ggtttgattg agccgagccg cacctgggcg aggacgggca actcggtacc gacggtgacc
      121 tcggcgggca ccttcgcgtc caccgattcg attcgcacct cgctccactt gtcggtgacc
      181 cgtcgccgcc agccggcgat gtcgcgggca atcttgtagt tatcctgcgt aaagcggtgc
      241 catcgacggg ctgcggggag gtagaagcgt tcggtatact cgcggaccat ccggttggtg
      301 ttgaagacgg gacagcaggt tcgcatggag gccttcatca ttgccgtcca gcgtcgtggc
      361 agaccgtcga cccccctgtc gtagaacatc gggatgagtt ccttttcgag cagttcgtag
      421 agcgattcgg cctccatgaa atcctggtaa tcctggttgt tgtagatccg gcgatcgccg
      481 atcgcccaac cattggtgcc gtcataagcc tcgggccacc agccgtcgag caccgacatg
      541 ttgattccgc cgttgggggt gaccttcatg ccgctggttc cgctggcttc gtggagtttg
      601 gtgggcgtgt tgagccagac gtcgacgcct tcgatgagca ggcgggcgac gctcatgtcg
      661 tagtcttcaa gaaagatgat gcggttgcgg acgcgcggat ccttggtgaa cgccacgatc
      721 cgcttgatca gttccttgcc ggcttcgtcc tttggatggg cctttccggc gatgatgacc
      781 tggaccggcc gctgcggatc ggtcagcagc ttgcgcaaac ggtcctggtt gtagatcaac
      841 agcgtggcgc gcttatatgt tgcaaaccgt cgtgcgaagc ccagcgtcaa ggcctccgga
      901 tcaagcattt cctcgacggc gtccacctcg gccggcgggg ccccgcgggc gatcaactgg
      961 gccttcaatc gccgtcgtgc gaagcccacc agccgctcgc gcagccgctc gtgcgttctc
     1021 cacagctccg catccggaac cagatccact cgttgccaga tcgcctggtt caacgggtct
     1081 tcggcccatt gtggtccgag atactggtcg agcaagtggg ccatgtcacg tgacaaccag
     1141 cttcggatgt gaacgccgtt ggtgattgcg gtgatcggca cctcgttggt gggcacgccc
     1201 ggataaaccg gcccgaacat cttgcgggag acttccccgt gaagctggct cacgccgttg
     1261 ctcagatagg atagtcgcaa agccagaacg gccatggaga acggctcgtg ggtgtcttcg
     1321 ggctgcattc ggccgagttg gagcagcgtc tgctccgtga ttcccagtat ctggcagtag
     1381 ggcgtgaggt acttggtaac cagggccggc gggaagtggt cgatcccggc gggaaccggc
     1441 gtatgggttg tgaagacggt gcctgtcgag acgacctcgc gggcctgctc gaaagtgagc
     1501 ttatcttctc gcatccgctg atggatacgt tcgacggcga ggaacgcgga atgcccctcg
     1561 ttcatgtggc agaccgacgg tgtgatcccc agcgccgcca gggcccgcat cccgccaatg
     1621 cccaggacga tttcctgtcg gatgcggact tcctggtcgc cgccataaag ccgggcggtg
     1681 atgtcacgat cctcgcgccg gttattggga tggttggaat cgaggaggta aagaatcacg
     1741 cgtccgacgg tcactttcca gaccttgatc catacgtttc gtccgcccac gtccagttga
     1801 acacataatt ggttgccgga cgcatccagg acgggggtga ccggcatggt cgcaaaatcc
     1861 agctcggggt agtcctcaaa ctgatagccg tcgttggtca gatactggtg gaagtatccc
     1921 tgtcgataca ggagcccgac cccgtacagc gggagtccga ggtcgctggc actcttgaga
     1981 tggtcgccgg agagaatccc caggccaccc gcgtagatcg gcaggcactc gtgcaacccg
     2041 aactccgcgg agaagtaagc aattttcccg atgtcctcat ggccgtaagt ctcagagaac
     2101 cacgtagggc gctccaggta tgcagcgagt tgatcctgaa cacggcggag ggcggcgatg
     2161 taggccggat cgcgggccaa ccggtcgagg cgctcctgcc ggagttgggc cagcatggca
     2221 accgggttgt gtttcaattc cctccagagg tcgacgtcca ggcggcggaa gagctcgatg
     2281 gcatcggggt tccatgtcca ccagacgttg tacgccaagt ccctcagggg cttgagggcc
     2341 tcgggaagag ccggcagtac catgaagctg cgaacatgag gcatagtgta gcaccataca
     2401 tcaaggcaag aagctagtcg ggcacgcgca cacgtgcacc gtccgggtca gttccggctc
     2461 gctcgccgtg ggcgcatctc gtcccgcagc aacgatttcc ggcacattgt agcatcggca
     2521 gaaacccgtt accggtcacg ataaaactac tctgcatcag gggtgttcgg gctgtcaaga
     2581 ctgtgtcttc actggcgggg aagcgcttgc gacggaatgc cgtcttacgg gaatcgggtt
     2641 gctgagcata cggtttatcg cctattgttc aggctggctg gaacagagag ccggcatgtt
     2701 ggttcgagtg ctgttattcg tttgttactc ttttcaagat aggcagccaa caggtcggag
     2761 cctttgtgtt gggttacccg acctgctgag acggttgggt cgggatgggg cggtccaccg
     2821 ggcatccacg ttcactttcg accggtgcgg actcgcgaga ggttggtttg accttgttgc
     2881 ggctttggac ggacctggtt ttgcgtgggg ttgtttgccc cattacgtcg ctcatgcggc
     2941 ttggcgagcc tgcgttggga ctgcgggggg ccgggcgcgc aaccgctgcc cgcaaacagc
     3001 aagagccata gccaagtttg tccacgcgcc gaggctcaga tccgcaaaga ggcccaagag
     3061 aatcaatgat agcagccggt cggatcggtc gaaaacggcc tgttcgcgtc gggctgtttt
     3121 tttcgggccg tgcagaccga gccacgttgc tccgaaggcg gcggcgatca cggcaaggct
     3181 cagccaaaag ggtatcaatc cgccgaatgc gggcccggtg aagatgatcg cttcggagag
     3241 tttgtccgtg agtgcgtgcc tcgtgatgct gctgccggcg gcgccggccc gcgcgagtgc
     3301 tccgtcgagg taatcaaaac cgccgtggag gatcaaggcc acgagaccgg caagtcgcat
     3361 gccggaggcg aacaggcttg ctgccaggat gcccatagcg acttccagca catttgcggt
     3421 gaaaggcctg aggcccagtc gatgcaagcg ggaggtcgcg ctggtgaaaa gacggtcacc
     3481 cgttctgacg atcagtgggc cgagcatgtg cctgtttctc ccgcaggctc acgggatgct
     3541 ctcgggcatc gggggtccgg tccgtaggca ctcccgccga gcgcccgggc atacgcatga
     3601 catcctccg
//
LOCUS       DAOTPW010000374         3591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_20589_length_3591_cov_4.990950, whole genome shotgun sequence.
ACCESSION   DAOTPW010000374 DAOTPW010000000
VERSION     DAOTPW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20589_length_3591_cov_4.990950"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="P5316_21060"
     CDS             <1..1107
                     /locus_tag="P5316_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87503.1"
                     /translation="AGLRNTMATCFPEVIVGWGDPAHILAATQKDLVSTDPVQLTERY
                     QQRHIEPVGFDPVWFSGAVDWFAAEKVAQRAAELDAVVGAVVSTDQRPIVYMDRLALW
                     EKMTAAHSRRFIEPLLGVKWWQAAAFLAAAALIILLSYKALGRSISEAVVVFSVGSTG
                     FATMSLSIVWLFAFQNLYGYVYSWIGWIVAVFMGGLVVGCAGAGRRASPAASVTSADQ
                     GPPPVVCLSGLTLLKPGPPVHASPRRRLIIVDLLLAILAVLAPLLLPVLGQAQTTPAA
                     LVLVQVSILVLVLMTGILGGAAFALASGLHQMVSGRPMATAGTIVGGDHAGACLGALI
                     TGILLVPVLGIVTTSLLLAGTKCVSACLLMSARK"
     gene            1535..2950
                     /locus_tag="P5316_21065"
     CDS             1535..2950
                     /locus_tag="P5316_21065"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRR87504.1"
                     /translation="MKRASISQHCRAAIALLSLASAFSAADGRPNFVFILVDDLRWDA
                     MSCAGHPFVRTPNIDKIASAGTRFTNAFVTISLCAPSRACFLTGTYAHINGVRTNEQM
                     EFDHSMPTYPRLLQAAGYETAFIGKWHMKPVADPRPGFDYWLSFVGQGQYINPKLNEN
                     GRNFQAGGYITDLLTQYAVDFIKRPREKPFCLNLWHKAVHGPFTPADRHKDLYRDVLL
                     PEPPSFRDTFEGKPTWQSRRRSAAGDDSQATRPSLADLQPPTWNPRDEQKLNYFRALA
                     AVDDSVGRILEVLRETAVIDNTVIIFAGDNGYFHGEHRRGDKRAAYEESIRIPLVMCG
                     PGVPRNGGKLEPMVLNIDIAPTILDLAGVKPPASMQGMSLKPLLAGATPAWRKSFLYE
                     YFQEKQLSQVPTVAAVRTERWKYVTYPEIQDLDELYDLTRDPIEMHNLATDPAAKEQL
                     QKMRAELERLKKETGYTPFSR"
     gene            complement(2969..>3591)
                     /locus_tag="P5316_21070"
     CDS             complement(2969..>3591)
                     /locus_tag="P5316_21070"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87505.1"
                     /translation="QKEIARVHEVDPSLFEGLWDQGRAAEVTRRGLVLEQAPVMPGSD
                     AGRVQLPNLVEGEGFTIDMWIRFNELEFDQVILSGQNEEGSGLRLLVTKTGTIGLELS
                     DGKELRSVWDTDTRVIKADRLHHIVAIVEGGPKIIMFVVDGVLCDGGEDRQFGWGRFD
                     AGLTDLGGTLPLHVPSSLKGAVKSLRIYARPLRVSEAVSNYRAGLR"
BASE COUNT          690 a         1135 c         1057 g          709 t
ORIGIN      
        1 gccggtttgc ggaacacgat ggcgacctgc tttcccgaag tgatcgtcgg ttggggcgat
       61 ccggcacaca tccttgcggc cacgcagaaa gatctggttt cgaccgaccc ggttcaacta
      121 acggagcgtt atcagcagcg gcatatcgag cccgtcgggt tcgatccggt gtggtttagc
      181 ggcgcggttg actggttcgc ggccgagaag gtcgcccaac gagcggcgga actcgatgcg
      241 gtcgtcgggg ccgtggtgag cactgaccag cgcccgatcg tctacatgga ccggctggca
      301 ctctgggaga aaatgaccgc cgcccattct cgccggttca tcgaaccgct gcttggcgtg
      361 aagtggtggc aggctgcggc cttcctggcc gcagcggcac tgataatcct gctctcgtac
      421 aaagccctcg gccggtccat cagcgaagcg gtggtcgtct tttcggtcgg cagcaccggc
      481 tttgccacca tgtcgctgag cattgtgtgg ttgttcgcgt tccagaatct ttacggatat
      541 gtctactcgt ggatcggctg gatcgtcgca gtattcatgg gcgggctggt tgtcggctgc
      601 gcgggggccg ggcggcgggc atcgccggcc gcctcggtca caagcgcaga tcaagggccg
      661 ccgccggtgg tttgcttgtc cggcctgacc ctgctgaaac cgggcccacc agtgcatgcc
      721 tcaccgcggc ggcgactgat cattgttgac ctgctgctgg caatcctggc cgtactcgca
      781 ccgctgctac tgccggtcct aggccaagcg caaaccacgc cggcggctct ggtcctggtt
      841 caggtttcca tactggtgct cgtcctgatg accgggatac taggcggtgc cgccttcgcc
      901 ttggccagtg ggcttcatca gatggtctcc ggccgtccga tggccacggc cgggaccatc
      961 gttggcggcg atcacgccgg tgcgtgcctc ggcgctctca tcaccggcat tcttctcgta
     1021 cccgtcctcg gcatcgtgac aacctcgctt ctgctggccg gcacaaagtg cgtttcagca
     1081 tgcctgctga tgtcagctcg taaatgagcg atcacgtagg gatcgagaat tcgcagtggc
     1141 cacagggtcc tacaaccgac aatgacacat gtgctctcgt cgcctttccg accacgggcc
     1201 gacttgcagg tcggccgatg ccgctgtatt ctccggctca gtcataccgg atcaagcggt
     1261 taagcgagct gaggaacggc tcgtggcaga tgatttcgcc tgcaagacca tcgaccccat
     1321 gaaacccagc agacctgaaa tcgacgcgat cttatccggc ggcttgcttc tccggcacct
     1381 aagtattagc ctgtaagaca cttgaacttc atcgcggcca agttcgtacc ggagaatacc
     1441 gaaccgatct tcttctctgg cgtacgcgag tcaaacgaag gagggtgatg ctgcacggca
     1501 tgtccggctg agtgtatatt tgaggagaac agccatgaaa cgtgcatcta tcagtcagca
     1561 ttgcagggca gctattgcgt tgctttcact ggcaagcgcg ttttcggccg ccgatggccg
     1621 gcccaatttc gtgttcatcc ttgttgacga cctgcgttgg gatgccatga gctgcgcggg
     1681 gcatccattc gtccggaccc ccaacattga caagatcgct tcggccggca ctcggttcac
     1741 aaatgccttc gtgaccattt cactgtgcgc cccatcccgg gcctgttttc tgacgggaac
     1801 ctatgcccac atcaacggcg tacgaaccaa cgaacagatg gaattcgacc actccatgcc
     1861 gacctaccca cggctgctgc aggcggccgg ctacgagacc gctttcatcg gcaagtggca
     1921 catgaagccg gtggccgacc cgcggccggg gttcgattac tggctcagct tcgtcggcca
     1981 gggccagtac atcaatccga aactcaacga gaacggccgc aactttcagg ccggcggtta
     2041 catcacggat ctcctgacgc agtacgctgt tgacttcatc aagcggccgc gagaaaaacc
     2101 gttctgcctg aacctctggc acaaggcagt gcacggaccc tttactccgg ccgatcggca
     2161 caaagacctc taccgcgatg tgctcttgcc cgagccgccc agtttccgcg ataccttcga
     2221 gggtaagccg acctggcaaa gtcggcggcg atccgcagcc ggagacgata gccaggcaac
     2281 ccgcccgtct ctggccgacc tgcaacctcc gacatggaac ccgcgggacg agcagaaact
     2341 caactacttc cgtgcgctgg cggccgtgga cgacagcgtg ggtcgcatcc tcgaagtgct
     2401 gagggaaacg gctgttatcg ataacacggt gatcattttc gccggcgaca acgggtactt
     2461 tcacggcgag caccgtcgcg gtgacaagcg ggccgcatat gaagaatcga tccgcatccc
     2521 gctggtcatg tgcgggccgg gggtccccag gaacgggggc aagctcgaac ccatggttct
     2581 caacatcgac atagccccga ccattctgga tctcgccggt gtcaaaccac cggcctcgat
     2641 gcagggcatg tcactcaagc cgctgctggc cggcgcgaca cccgcctggc gcaagtcctt
     2701 cctgtatgag tacttccagg agaagcagct ttcgcaggtg cccacggtag cggcggtccg
     2761 tacggagaga tggaagtacg tgacctaccc cgagatccag gacctcgacg agttgtatga
     2821 cctgaccagg gacccgatcg agatgcacaa tctcgcgact gatccggcgg ccaaggagca
     2881 attgcagaag atgcgggccg agctcgaacg gctgaagaaa gaaaccggat acacgccttt
     2941 ctcaaggtag ttgtccgggc gcggcgcgtc agcgaagccc tgcgcggtag ttggacactg
     3001 cctcggagac tcgcagcggc cgcgcgtaaa tccgcaggct cttgaccgct cccttcaacg
     3061 acgagggaac gtgcagcggc agggtgccgc cgagatcggt caagccggcg tcgaagcgac
     3121 cccatccgaa ctggcgatct tcgccgccgt cgcagagcac tccgtcgacc acaaacatga
     3181 tgatcttcgg tccgccttcg acgatggcaa caatgtggtg caatcggtcg gccttgataa
     3241 cccgcgtatc agtatcccaa acgctccgca actcctttcc gtcgctgagt tcaagaccga
     3301 tggttcccgt tttcgtgacg agcagcctga ggccggatcc ttcctcgttc tgcccgctca
     3361 ggatgacctg gtcgaattcc agctcgttga atcgaatcca catatcgatg gtgaagcctt
     3421 caccctcgac aagattcggc aactggacgc gtcccgcatc gctcccgggc atgacagggg
     3481 cttgttcgag aaccagtcct cgcctggtca cctcagccgc ccgaccctga tcccagagcc
     3541 cttcaaacaa cgaaggatcg acctcgtgaa cgcgggcgat ctccttttgc g
//
LOCUS       DAOTPW010000375         3589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_20607_length_3589_cov_5.071873, whole genome shotgun sequence.
ACCESSION   DAOTPW010000375 DAOTPW010000000
VERSION     DAOTPW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20607_length_3589_cov_5.071873"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            611..1810
                     /locus_tag="P5316_21075"
     CDS             611..1810
                     /locus_tag="P5316_21075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRR87506.1"
                     /translation="MWSSLLAESGVLAGAWWEYLARNWALASIVSTLACLLLISWLIL
                     RKYIKIMFNIIRDTPPPLAMGPCDFERIDGERVVFRAFDNIHLCGMFLFGDHGGHPKG
                     MVIFAHEFSSDMYSCARYCRPLLKAGYNVFSFDFRGHGESSLQPGYQPRQWPTNHELN
                     DMLGAIAYVEDWLESRGFPLEVGLFGISRGAGAAILAAQNNKTVKAIIADGAFSSDTT
                     LEHLMKRWAYIFAKVRFVYENHPPQFWRFLRWLLFCECRKRLNCTFPSVRKALARMEG
                     PRPILFIHGERDSYIPVEQSQLLYESAPGPKFLWIVPGAKHNQSAVLRPDQYAARTVA
                     FFDYYLAGAGTEQAVERVLRSDSHSGEPVKGLSGVAGQRNVFSRLDRRRRARNNSNRQ
                     VARSADA"
     gene            2347..>3589
                     /locus_tag="P5316_21080"
     CDS             2347..>3589
                     /locus_tag="P5316_21080"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRR87507.1"
                     /translation="MARSGLSEVGGIRRGHAAREERIRGLRFSRWLGCPVAVCVVMLL
                     GLLTGSAHAQSVCNVGSGVTVEWDPATGVVLITGDDGSNIIVLDVVEVTEGEFREAVE
                     EDLTADPPIVGVPADPPADAYYLTVNGQHCPPPPVPAPGSAGIPANQVSAIIVLTFGG
                     NDIISAINGDGSEGLLDVTDEDPPLMYGLSIYVDAGEGNDRIFGGHGDDIIFGGPGDD
                     FIHGDRGADTLDGGDGVDTLDYGLIGDSDVEGAQGAFVDLPNDTATDPTFPFFGFPAA
                     TTDTLVGTSFEIVYGCERNDVIIGNTDLSTRIYGLEGDDRLTGGNAGDFIFGGEGNDT
                     IDAGEGDNGSEEEPIDGGEGDDTVVAGSGNDWLGGGAGNDMIESGDGNDVVFGDGLNP
                     NDDQVSGNDQIRLGDGNDVAFG"
BASE COUNT          621 a          934 c         1262 g          772 t
ORIGIN      
        1 ccgagcgcga catggtctgg tggaaagggc cgacgagggc gtcggcccca cttgtggccc
       61 gatccgtggg cgacatggtc tggcggaaag ctcgagagaa aaatcgcacg ttgattctgc
      121 ctgttctggt tgccgaagtg ctgatcgttg gcggcggacg ttctaagatc ttgtgagatg
      181 gccacttccg cctgtgtcgg taattcgtca cttctcatcg tggtattggc tgagattttt
      241 ggtttttgca cttgtcagtg tcggtaagtt gcgttaatct ttagggttgg cgggagagta
      301 tagccatcgg agagagagtg gctccaaggg caggagcctg tgagccttct cagagcggtc
      361 gagctgaggc cgcaatctcc gcagttatca gtcgttgaga atgaacgcct tcgggcgtag
      421 ttcatagtgc ttttcccctc cggaaagcac cgggtagtct tcggactccc ggtgttttct
      481 ttttggtgtc gagggtgttc ggtagtcggc gggcgcgcgg ttcgtgtgac gggcttgctc
      541 gtcgcgatca ggtaagctta ccataccgcc ggcgtggcgg cggggtggca gctaagctgc
      601 gaagctgagg atgtggtcca gcctgttagc ggagtccggg gtgttggccg gcgcatggtg
      661 ggagtacctg gcgcgcaact gggcccttgc ctcgattgtg tcgaccctgg cctgcctgct
      721 gctgatctcc tggctgattc ttcgaaagta catcaagatc atgttcaaca tcatccggga
      781 taccccgcct ccgctggcga tggggccgtg tgattttgag cggatcgacg gcgagcgggt
      841 ggtcttccgg gcgtttgaca acattcacct gtgcgggatg ttcctgttcg gcgatcacgg
      901 ggggcatccc aagggcatgg tgattttcgc tcatgagttt tcgagcgaca tgtacagttg
      961 cgcgcggtac tgtcgtccgc tgctgaaggc gggttacaac gtgttcagtt tcgactttcg
     1021 gggtcacggg gagtcgagtc ttcagccggg ttatcagccg cggcagtggc cgacgaatca
     1081 cgagctgaac gacatgctgg gggccattgc ctacgtggag gactggctgg agagccgagg
     1141 gtttccgttg gaggtgggtc tgttcgggat ttcgcgcggg gcgggggccg cgattctggc
     1201 cgcccagaac aacaagaccg tgaaggcgat catcgccgac ggggcgttca gctccgacac
     1261 gacgctggaa catctgatga agcgctgggc ctatatcttt gccaaggttc gctttgtcta
     1321 cgagaaccat ccgccgcagt tctggcgttt cctgcgttgg ctgctgtttt gcgagtgccg
     1381 caagcggctc aactgtacct ttccctccgt ccgaaaagcg ctggcccgca tggaaggccc
     1441 gcggcctatt ctgttcattc acggcgagcg ggacagctac attccggtgg aacagtccca
     1501 gttgctatac gagtcggctc ccggtccgaa attcctgtgg atcgtaccgg gtgccaaaca
     1561 caaccagtcg gccgttttgc gtcccgacca gtatgcggcg agaacagtcg ctttttttga
     1621 ctactacctg gccggggccg gcactgagca ggccgtggag cgggtgctga ggtccgattc
     1681 gcattcgggg gaacctgtga aaggcctgtc cggcgtcgcc gggcaaagga atgtgttcag
     1741 caggcttgac cggaggcgtc gagcgaggaa caacagtaat agacaggttg cccggtcggc
     1801 ggacgcgtag ggttcgcgag gaccggtaac tgcgggcttg cggccgagga gggggagacc
     1861 tgccaggagc cgcagccgcg caacgcgggt gagggtgtct ctcgcggttg cggtgggagg
     1921 gggcagggcg attcccgcaa gtgttggtat actgcgtgat tagggtccgg cgcgctggat
     1981 gggatggtct ccatgtgaat ccggtgtgcg gggtcggggg gcagcggatt tcgggagcag
     2041 gcgggggctg agcagccggc ccgataacgc cgcttgaatc ggcggcagcg ctaagttgct
     2101 tggttgcgat gagttggagg tttataccgg gcgagatccg ggtgttgggt gagcggcccg
     2161 aacctgggcc attggcgaga catttgtgcc cggttacgat tgacgaagga tggcttgcat
     2221 ccagggtggc atactgcatg cgtacagggg ggggcggcgg tgtctcgcac ttaggttccg
     2281 atcatggaca gtcggtgcgg ggcctgtgat aagtgattgc ggttcaagag aataaggttc
     2341 attccaatgg ctagaagcgg attgagcgag gtgggcggga ttcgtcgtgg tcatgctgcg
     2401 cgcgaggagc gaatccgtgg gttgcgattc tcccggtggc tgggctgccc agtggcggtt
     2461 tgcgtcgtga tgctcttggg cttgctgacc ggttcagctc atgctcagtc cgtgtgcaac
     2521 gtgggttcgg gcgtgaccgt tgagtgggat ccggcgactg gcgtcgtgct cattaccggc
     2581 gatgacggaa gcaacattat cgttctggac gttgttgagg tcacggaagg cgagttccgc
     2641 gaagcggtcg aggaagacct gacagcagat ccgccgatag tgggtgttcc ggccgatccg
     2701 cctgccgacg cctactacct caccgtcaat ggccagcact gtccgcctcc gccggttccc
     2761 gccccgggtt ccgcaggtat tccggccaac caggtgagtg cgatcatcgt cctgaccttt
     2821 ggcggcaacg acatcatcag cgcgatcaac ggggatggaa gcgagggctt gctcgatgtc
     2881 accgacgagg atccgccgct catgtatggg ctcagcatct acgttgatgc gggtgagggt
     2941 aatgacagga tcttcggcgg gcacggcgac gacatcatct tcggcgggcc gggcgacgat
     3001 ttcatacacg gcgaccgcgg ggcggacaca ctcgacggcg gcgacggcgt tgacaccctc
     3061 gactacggtt tgatcggcga tagtgacgtt gaaggcgcgc aaggcgcttt cgtcgacctg
     3121 ccaaacgaca cggcgaccga tccgactttt ccgttcttcg gcttccccgc cgccacgacc
     3181 gatacgctgg tggggacaag cttcgagatc gtttacggct gcgagcggaa cgacgtcatc
     3241 atcggcaata cggatctttc taccagaatc tacgggcttg aaggcgacga ccggttgacc
     3301 ggcggcaatg cgggcgactt catcttcggg ggcgagggga atgataccat cgacgccggc
     3361 gagggcgaca acggttcaga ggaagagccc atcgacggcg gggagggtga tgacaccgta
     3421 gtggccggtt ccggcaacga ttggctcggc ggcggcgcgg gcaacgacat gatcgagtcg
     3481 ggcgacggta atgacgtggt gtttggcgac ggtctgaatc ccaacgacga tcaggtttcc
     3541 ggcaatgacc agatccggct tggcgatggg aacgatgtgg cttttggcg
//
LOCUS       DAOTPW010000376         3567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_20748_length_3567_cov_5.210137, whole genome shotgun sequence.
ACCESSION   DAOTPW010000376 DAOTPW010000000
VERSION     DAOTPW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20748_length_3567_cov_5.210137"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="P5316_21085"
     CDS             complement(<1..260)
                     /locus_tag="P5316_21085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009302919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRR87508.1"
                     /translation="MRVFSLCQPLLALTLAPLLLGIISRVKAFVAGREGPPLIQPYLD
                     LFRLLGKGAVYSRTTTAVFRLAPVVGLAAVAAAVLLLPIGGT"
     gene            complement(264..2252)
                     /locus_tag="P5316_21090"
     CDS             complement(264..2252)
                     /locus_tag="P5316_21090"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRR87509.1"
                     /translation="MVFLLTGIAIMMAGGLAALLVPRRGHWASTLGSTCVVAGCITGL
                     IPAVRILLGAPAEVLHRSWSVPYGSFHVELDALSAFFLIPILLLCALAAIYGIEYLRP
                     YRDRKSLGASWFFFSLLVASMILVTTARNIMLFLVAWEVMSLASFFLVTFEHEDEKVS
                     QAGWTYLVATHLGTLFLLAMFALLGLHSGSPELAGGTSPVKAVADHANLLFILAIIGF
                     GTKAGFMPFHVWLPEAHPAAPSHVSAIMSGVMIKTGIYGLLRTLTFLGPPPAWWGWLL
                     IAIGLTSGVLGVLFALAQHDLKRLLAYHSVENIGIIAIGLGVGLLGLSLHSVSMAVLG
                     FGGGLLHVLNHACFKGLLFFGAGSVLHATGSREIDRLGGLLKRMPWTGWTFLVGAAAI
                     AGLPPLNGFVSELLIYLGALEGVRTTSPLHLLLMAGAVAGLALIGGLALACFAKAFGV
                     VFLGEARSADAGGGHECGWLMRVPMLVLAAACVAIGLLGPLAVLSLARAIAVVGGMPE
                     NVVRATIANPRDVLIAVVGVSFILLLLTAGLIVFRRRLLEGRMVSSTVTWDCGYARPT
                     ARMQYTASSFAQPLTRFFQPFLRTRTALTPPVEYFPPSASLATHTDDVWREKGYSPVF
                     RGAAWVFSKLRRLQHGRVQTYVLYIAVTLLALLLWKLR"
     gene            complement(2255..2971)
                     /locus_tag="P5316_21095"
     CDS             complement(2255..2971)
                     /locus_tag="P5316_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRR87510.1"
                     /translation="MTITVRDAAKLLNVSERTIYRWIDSGSLPVYRVGDQFRFNRTEL
                     LEWATSKKLNVSAEIFAEPEHDQETPCRLSEALRAGGIFYRIEGSDKESAIRSVVDIL
                     RLPDDVDREFLYQVLLARESLGSTGIGEGIAIPHVRNPVVLHVTQPSVTLCFLEHPIE
                     FGALDGQPVHTLFTLISPTVRSHLRMLSLLSFALRDPAFKETLRSQALREAILTEVMR
                     IESSLASADQPLSPGGAESR"
     gene            complement(3147..>3567)
                     /locus_tag="P5316_21100"
     CDS             complement(3147..>3567)
                     /locus_tag="P5316_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR87511.1"
                     /translation="ARTARSGGIRRALRRRSRAPRASCPYQHHARQLPVIGPLFVNII
                     LVGMSAQTGADFFHTSAVEAVSRLGSRPVMVIHGDDDIAMPVSHSQALYDAAAGPREL
                     WFGPGPHSNIVTTDPRQYEQRVFALLDANLSEHSSQK"
BASE COUNT          810 a         1095 c         1100 g          562 t
ORIGIN      
        1 ctggttccac cgatcggaag cagcagcacc gcagccgcca cggccgccag cccgacgacc
       61 ggagccagtc ggaaaaccgc cgttgtcgtc cggctgtaca ccgcgccctt gccaagcagc
      121 cggaacaggt cgagataggg ctgaatcagc ggtggccctt cccgtccggc gacgaacgcc
      181 tttaccctgc tgatgatccc cagcaggagc ggggccagcg tcagggccag tagtggttgg
      241 cacaaactga agactcgcat ggcctatctc aacttccaga gcagcaaagc caacagcgtg
      301 acggcgatgt acaagacgta agtctggact cgaccatgct gaagccggcg cagcttcgag
      361 aacacccacg ccgccccgcg gaagaccggg ctgtatccct tctctcgcca gacatcatcg
      421 gtgtgtgtgg caagagaagc cgacggggga aagtactcaa ccgggggagt cagggcggtg
      481 cgggttctca ggaacggctg gaagaaacgc gtgagcggtt gggcaaaaga cgacgcggta
      541 tactgcatgc gggcggtcgg gcgcgcgtaa ccgcaatccc acgtcacggt gcttgagacc
      601 atacgaccct caagaagccg gcggcggaag acgatcagcc cggccgtgag cagcagcaga
      661 ataaaactga cccccacgac cgcgatgaga acgtcgcgag gattggcgat cgtcgcgcgg
      721 acaacgttct caggcatgcc gccgacaacc gcgatggccc gggcaagtga gagaaccgcc
      781 aacggtccca gcaaaccgat cgcaacgcac gcggcagcca aaaccagcat cggtacgcgc
      841 atcagccagc cgcactcgtg gccgccgccg gcgtccgccg atctcgcctc gccgaggaaa
      901 accacgccga acgccttggc gaaacaagcc aaagccagtc cgccaatcag cgccagcccg
      961 gcgaccgcgc ctgccatgag aagcaggtgc agcggcgacg tcgtccgcac gccctccagc
     1021 gcccccaggt agatcagcag ctcgctcacg aaaccgttga gcgggggcaa gccggcgatc
     1081 gccgcagccc cgaccaggaa ggtccagccg gtccaaggca ttcgtttgag gaggccgccg
     1141 aggcggtcga tctctcggct accggtggcg tgcagcaccg atccggcccc gaaaaacagc
     1201 agccctttga agcacgcgtg gttgagcacg tgcagcagcc cgccgccgaa gcccaggaca
     1261 gccatcgaaa ctgaatgcag actgagcccc agcagtccga cccccaggcc gatggcgatg
     1321 attccgatgt tctccacgct gtggtaggca agcagacgct tgaggtcgtg ttgtgccagg
     1381 gcaaacaaga ccccaagcac gcctgatgtc aacccgatgg cgatcagcag ccagccccac
     1441 cacgcaggcg gcggcccgag gaacgtcagc gttctcagca atccatagat gccggtcttg
     1501 atcatcacgc ccgacatgat cgcggacaca tggcttggag ccgccgggtg ggcctcgggc
     1561 agccacacgt gaaagggcat gaagcccgcc ttggttccga aaccaatgat ggccagaatg
     1621 aacagcaggt tcgcgtggtc cgcaaccgcc ttgactggag aggtgccacc cgcgagttcc
     1681 ggcgatcccg agtgcaatcc cagcagcgca aacatcgcca gcaggaacag cgttcccaag
     1741 tgcgtggcca caaggtacgt ccagccggcc tggctcacct tctcgtcctc atgctcgaat
     1801 gtcacaagga aaaacgaggc cagcgacatg acctcccacg ccacgaggaa caacatgata
     1861 ttgcgcgcag tggtgaccag gatcatgctg gcgaccagga gactgaagaa gaaccacgac
     1921 gcgccgagcg acttgcgatc gcgatacgga cgcaagtact cgatgccgta gatggctgcc
     1981 aaagcacaca gcaggaggat cgggatgagg aagaacgcgg acaacgcgtc caactcaacg
     2041 tgaaatgaac cgtacggtac cgaccaggac cgatgaagca cttcagcggg tgctccgagc
     2101 aggatacgaa ctgccggtat cagcccggtg atgcaaccgg ccacaacaca ggtggatccc
     2161 agggtagacg cccaatgccc ccgacgcggc accaacaggg ccgcaagccc accggccatc
     2221 atgatggcaa tgcctgtcag gagaaaaacc atgttcatct gctttcggca ccaccgggtg
     2281 agagaggctg gtccgcggat gcaagggatg actcgattcg catcacctcg gtcaagatcg
     2341 cttctcgcag agcctgcgat ctgagcgtct ctttgaacgc gggatcgcga agcgcgaacg
     2401 agagcaagga aagcatccgc aggtgcgagc ggacggtggg actgatcagt gtgaacagcg
     2461 tgtgtaccgg ctggccgtcg agcgccccga actcgatcgg atgctccagg aaacacagcg
     2521 ttacggaggg ctgggtaacg tgcaaaacca ccgggttgcg aacgtgcggg atggcgatgc
     2581 cttcgcctat ccccgtggag cccagcgact cgcgggccag cagcacctgg tagagaaact
     2641 cgcggtccac gtcgtccgga agccggagaa tatcgacgac agatctgatc gccgattcct
     2701 tatctgagcc ttcgattcga tagaagatcc cgccggctct gagggcctcc gaaagcctgc
     2761 aaggagtctc ctggtcatgc tcgggttcgg cgaagatctc cgccgagacg ttcagtttct
     2821 tcgatgtggc ccactcgagc agctcagtcc tgttgaacct gaactgatcg cccacgcgat
     2881 agacgggcag agaaccactg tcgatccacc ggtagatcgt tcgttccgat acgttcaaga
     2941 gcttggcggc atcccgcact gtgattgtca ttagatccct ttgtcggtca ttagtgacgc
     3001 aaagagacat cataagacat gctatgcaca atgtcaatag aaatagatta tcgtgttttt
     3061 ttgtcagtgg tttgtccccc aagccttact tcgtcttcga atgtcgcaat atgcgacacg
     3121 aattcctgcc gctgattgtc cgtaagtcat ttctgagacg aatgttcgga tagattggcg
     3181 tccaggaggg cgaagacccg ctgttcgtat tgtcttgggt cggtggtgac gatattgctg
     3241 tgtgggccgg ggccgaacca gagctcgcgc gggccggcgg cggcatcata gagcgcctgc
     3301 gaatgcgaga ccggcatcgc gatgtcgtcg tccccgtgaa tgaccatgac agggcggctg
     3361 cccagtcgag acacggcctc cacggccgac gtgtggaaga aatcagcacc cgtttgggcg
     3421 gacattccaa ccagaatgat gttgacgaac aacgggccga tgaccggcag ttggcgggcg
     3481 tggtgttgat agggacagct tgcgcggggt gcgcggctgc ggcgacgcag agcgcgtcga
     3541 ataccaccgg aacgagcagt acgggcg
//
LOCUS       DAOTPW010000377         3529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_20993_length_3529_cov_4.445596, whole genome shotgun sequence.
ACCESSION   DAOTPW010000377 DAOTPW010000000
VERSION     DAOTPW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20993_length_3529_cov_4.445596"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="P5316_21105"
     CDS             complement(<1..297)
                     /locus_tag="P5316_21105"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRR87512.1"
                     /translation="MSDFSERRIALVGGAGFIGHHLALRLKSLGAEVLVVDSLAVNNL
                     YSLHREGRTNANAELYLRVIRERLDLLEDRAIPLEVMDARDYNGLSQVLASFDPD"
     gene            complement(297..1418)
                     /locus_tag="P5316_21110"
     CDS             complement(297..1418)
                     /locus_tag="P5316_21110"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR87513.1"
                     /translation="MIDEAERRAVAEVLNDVTLTHGPRVKEFERAFAAFTGAPAAVAT
                     ASCTASMHLVYMHLGLGPGDEVIVPTQTHTATAHCVELVGARPVFVDSEPKTGNIDIS
                     RIEPLINERTKALAVVHYLGLPVDMDAVMDMARRHNLFVVEDCALALGTYYRGIHAGL
                     LGDVGCFSFYPVKHITTGEGGMVISRHRTVNESVARLRAFGIDRNVVEDRRVPGMYDV
                     QGLGLNYRLNEIGAAMGIEQMKKLPEFLRRRRENHEALEAALKEIPGIELLQSTHEHF
                     QSSYYCMVMLLQPKWAERRPEIMEALKARGVGTSVYYPKPVPRMTYYRRKYGCAEDAF
                     PVASMISDRGVALPVGPHVNREDVDYMVSAIEEVLSGIN"
     gene            complement(1455..2459)
                     /locus_tag="P5316_21115"
     CDS             complement(1455..2459)
                     /locus_tag="P5316_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008313758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87514.1"
                     /translation="MSKLRVGIIGLGVGERHIAGYREHPGCEVVALCDFNEDKRREVA
                     ARHPGVAMKANAEEVLDDPSVDVVSIATYDHFHFEQAARAIRNGKHVFVEKPMFLREE
                     EGRQLRTLLEEHPGTVLSSNLVLRMSPRFTSLKNMIDTGKLGELYYAEADYNYGRLHK
                     LTQGWRGQATYYSVVHGGAVHMVDLLLWLTGDTIVEVQAYGNRICSKDSAFRFDDMVV
                     ACLRFESGMVGKVTANFGCVMPHFHGLTVYGSRGTFVNDRDCARLYQSREPADPVQEI
                     RTEYPGVHKGDLIRSFVNAILEGTRPAVTADDAFRAMSICVAIERATRQAGAVEVNYL
                     "
     gene            complement(2456..3154)
                     /locus_tag="P5316_21120"
     CDS             complement(2456..3154)
                     /locus_tag="P5316_21120"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRR87515.1"
                     /translation="MFVQARMTSQRLPGKVMLDVAGRPLLAYLMERLAHCHRVSTVIV
                     LTSRDASDDPVADFCQQRALKCFRGDLDNVARRFADALDHYGFEGFVRISGDSPLLDQ
                     ALVDRSVDAFLTGNHDLVTNVFPRSYPTGQSVEVVSAPAFRRALSRMSKPSHVEHVTS
                     YFYENHQAFSILNISAPAQLREIRLSVDTRADYEVFCRIVGLMEKPHWQYSLSEVVEL
                     YRRGAAQAGLAALT"
     gene            3415..>3529
                     /locus_tag="P5316_21125"
     CDS             3415..>3529
                     /locus_tag="P5316_21125"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR87516.1"
                     /translation="MPAYNAGRTLACCLASVLRMRPRPDELIVVDDGSTDDT"
BASE COUNT          689 a         1121 c         1020 g          699 t
ORIGIN      
        1 gtccgggtcg aaggaggcga gcacctgcga caggccgttg tagtcgcggg catccatcac
       61 ctccaacggg atggcacggt cctccagcag gtcaagccgt tcgcggatta cccgcaggta
      121 gagttcggcg ttcgcgttcg tcctgccttc acgatgcagg ctgtagagat tgttgaccgc
      181 cagactgtcc accacgagca cctcggcacc gagactcttg agcctcaaag ccaggtggtg
      241 gccgatgaag cccgccccgc caaccaacgc gattcgacgt tcagaaaagt cgctcatcag
      301 ttgattccag aaagaacttc ctcaattgct gacaccatgt agtccacgtc ttcccgattc
      361 acatgcggcc caacgggcag tgccacgccg cggtcgctga tcatcgacgc gacgggaaac
      421 gcgtcctccg cgcagccgta cttcctgcga taataggtca tgcgcggcac gggcttgggg
      481 taatagacac tcgttccgac gcccctggcc ttgagggcct ccatgatctc gggccgtcgc
      541 tccgcccatt tcggctggag gagcattacc atgcagtagt aggagctctg gaaatgctcg
      601 tgcgtggatt gcaggagctc gatacccggt atttccttca acgcggcctc aagggcctcg
      661 tggttctcac gccgtcgccg caggaactcc ggcagcttct tcatctgctc gatacccatg
      721 gccgccccga tctcattgag tcgatagttc agcccgagtc cctgtacgtc gtacataccc
      781 ggcacgcgtc ggtcctcgac cacgttccgg tcgatgccga aggcccgcag tctggcgact
      841 gattcgttga ccgtccggtg gcgggagatg accatgccgc cctcgccggt ggtgatgtgc
      901 tttaccggat agaacgagaa gcagcctaca tcgccaagca gacccgcgtg aatgccccgg
      961 taatacgtgc cgagcgccag ggcgcagtct tcaacaacga acaggttgtg acgccgcgcc
     1021 atgtccatga ccgcgtccat gtccaccggc aggccgagat agtggaccac ggccaaggcc
     1081 ttggttcgct cattgatcag cggctcgatg cgagaaatgt cgatattgcc ggtcttcggc
     1141 tcagagtcca caaacaccgg cctggccccg acgagttcca cgcaatgagc cgtggcggta
     1201 tgcgtctggg tcggcacgat gacttcatcg ccgggcccga ggcccaggtg catatacacc
     1261 aagtgcattg atgcggtgca cgaagccgtc gccaccgcgg ccggcgcacc cgtgaaggcc
     1321 gcgaaggccc gctcaaactc tttcacccgg ggcccgtgcg tcagggtgac gtcgttcagc
     1381 acctcggcca ccgcccgccg ttccgcttcg tcaatcatcg gacgtccaaa cggtatctgt
     1441 ctcatcagct ttgcctagag atagttcact tcgaccgcgc cggcctgcct ggttgcccgc
     1501 tcgatggcaa cacagatgga cattgcccta aacgcgtcgt ccgcggttac cgccggccgg
     1561 gttccttcca gaatcgcgtt cacaaagctg cgaatcaggt cgcccttgtg aacaccgggg
     1621 tactcggtcc ggatctcctg caccggatcg gccggctcac gcgactgata caaccgggcg
     1681 cagtctcggt cgttcacgaa tgttccacgg ctgccgtaaa ccgtcagacc gtgaaaatgc
     1741 ggcatcacac agccgaaatt cgccgtcacc tttccgacca ttccgctttc gaaccgcagg
     1801 caggcgacca ccatatcatc gaaccggaag gccgagtcct tcgagcagat ccggtttccg
     1861 taagcctgca cttccacgat tgtgtcgccg gtcagccaca gcagcagatc caccatgtgg
     1921 accgcaccgc catgcacgac ggagtaatag gttgcttgcc cgcgccatcc ttgcgtcagt
     1981 ttgtgcagcc gtccgtagtt gtagtcggct tccgcgtagt acagctcgcc cagcttgccg
     2041 gtatcgatca tgttcttgag cgaggtgaac cgcggcgaca tccgcagcac caaattcgac
     2101 gacagcaccg tgccgggatg ctcctccagc agtgttcgta gttgtcgtcc ttcctcttcg
     2161 cgcaggaaca tcggtttctc cacgaatacg tgcttgccgt tgcggattgc ccgtgcggcc
     2221 tgctcgaagt gaaaatggtc gtaggtcgcg atcgacacca cgtcgacgga cggatcgtcc
     2281 agcacctctt ctgcatttgc cttcatcgct acgcccggat ggcgggcggc gacttctcgt
     2341 cgtttgtcct cgttgaaatc gcacagggcg accacctcgc agcctggatg ctcccggtaa
     2401 ccggcgatgt gccgctcacc aacccccaag ccgatgatgc ccactctgag cttgctcatg
     2461 tcaaagcggc caagcctgcc tgagccgccc cgcgccggta gagttcaaca acctccgaca
     2521 ggctgtactg ccagtgcggc ttttccatca gccccacgat ccggcagaag acctcgtaat
     2581 cagctctagt atcgaccgat aagcggatct ccctgagttg cgcgggtgca ctgatattga
     2641 gtatgctgaa tgcttgatga ttctcataga agtaagacgt cacgtgttcg acatgcgagg
     2701 gtttcgacat cctggacaat gcccgcctga aagccggcgc ggaaaccacc tcgaccgact
     2761 gccccgttgg gtaagatctg gggaagacgt tcgtgaccag gtcgtggttg ccggttaaga
     2821 aggcgtccac ggatcggtca accagggctt gatccagcag tgggctgtcg ccgctgatgc
     2881 gcacgaatcc ctcgaagccg taatggtcaa gggcatcggc gaatcgcctc gccacgttat
     2941 ccaggtcgcc ccgaaagcac ttcaaggccc gctgctgaca gaaatccgcc acggggtcgt
     3001 cggaagcgtc tcgtgacgtt aggacgatga cggtggagac tcgatggcaa tgcgccaagc
     3061 gctccatcag gtaggccagt aacggtcggc cggcaacatc gagcattacc ttgccgggaa
     3121 ggcgctgcga agtcattcga gcctgaacga acaccgggat ttctgcagcg caaccggcca
     3181 aaggtagctc ctggaatcgt gaccgtcgaa gctgaggcgg gatctggtac catcgcctga
     3241 cgggaggtcg gcgcggtaca atttgcacgt ccctgttctc agcttgtccg atagtacatc
     3301 ggcaactcca cgtggccggg tcgtctcggc gatggctgga aatgaacttg gtgatagcag
     3361 ttcgcctgat agggagcagg ggtggcgaaa aaccgacaga cagttagcct ttatgtgcca
     3421 gcttacaacg ctgggaggac gctggcttgt tgcctggctt ccgtccttcg gatgagacct
     3481 cggccggacg aactcatcgt cgtcgacgac gggtccaccg acgacacgc
//
LOCUS       DAOTPW010000378         3510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_21152_length_3510_cov_5.597395, whole genome shotgun sequence.
ACCESSION   DAOTPW010000378 DAOTPW010000000
VERSION     DAOTPW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21152_length_3510_cov_5.597395"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="P5316_21130"
     CDS             complement(<1..1184)
                     /locus_tag="P5316_21130"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRR87517.1"
                     /translation="MDQSVKNAVDSWINDPAIAEADRKEIRDLLAVNNERELTDRFYR
                     GLEFGTGGMRGVIGAGLNRMNIYTVGAAAQGLANYVRSQGDAARKAGVVVAYDCRRMS
                     DIFAERVACVMAANDIPAYLFDRLRPTPELSFAVRHLGCTAGVVITASHNPPQYNGFK
                     AYWSDGVSVVPPHDKNIMAEVARVGGFGNVKVMSPAEAKAKGLLKIIGREIDEAFLQA
                     VQTSCLNPQVCRRQGEGLKIVFTGLHGTGSVLTPEALRRRGFKQVIEVPEQAAPDGGF
                     PTVKSPNPEEGAALDMAVALAKKVGADLVIGTDPDADRVGIAVRAPSGGFELFTGNQT
                     AALLTYYICDELTKAGRFPKNAVLVTTIVSGDMMKTIAKAYGAEVVEVLTGFKWIGAK
                     VC"
     gene            complement(1370..2320)
                     /locus_tag="P5316_21135"
     CDS             complement(1370..2320)
                     /locus_tag="P5316_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR87518.1"
                     /translation="MDKDVILVTGGAGFIGSHLCLRLFDEGHHVVCLDNYFTGRLENF
                     GDRYQELVLSGRFEFLRHDVIEPIVVEVDKIYHLACPASPVHYQYNPVKTIKTNVLGT
                     YHMLGLAKRVRARILQASTSEVYGDPQVHPQAETYWGYVNPIGIRSCYDEGKRVAETL
                     MMDYHRQNKVDIRIARIFNTYGPNMAVDDGRVMSNFICQALRGEPITVYGSGKQTRSF
                     CYVSDLVDGLCRLMESEHIGPINLGNDSEITLLELVEAIEKAMGRSLRVEYRPLPQDD
                     PCRRRPDLTKARTLLGYEPKVPLEEGIRRTIEYFRRTVGC"
     gene            2689..3429
                     /locus_tag="P5316_21140"
     CDS             2689..3429
                     /locus_tag="P5316_21140"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRR87519.1"
                     /translation="MGLVKTVFHIHTDYSDDSNLSVDCLADEAMERGVGCVAITDHDS
                     IEGAMTLADSVGDDLQVIVGEEISTKDGHLIGLFLRDLVEPGMSVRQTAEAIREQGGL
                     VVVPHPYNRLFHCSLGDRVYDILDLIDAVEVANAQNLSPLPNRRSARFARRHGFPAVV
                     GTDVHHRGYLDTCYQIMPGFEGPSGFVTSLGRARLHVRSHPLSYFLRTAWLITGGKLG
                     LPLPAGYGANAGEVPVRSSTRQAVLSAE"
BASE COUNT          668 a         1042 c         1072 g          728 t
ORIGIN      
        1 tgacagactt tcgcaccgat ccacttgaac cccgtgagga cctcgacgac ctcggccccg
       61 taagccttgg cgatcgtctt catcatgtcg ccggaaacga tggtggttac cagaacggcg
      121 ttcttcggga accggccggc cttggtcagc tcgtcgcaga tgtagtaggt cagcaaagca
      181 gccgtctggt tgccggtgaa cagctcgaat ccgcccgagg gagcgcgaac ggcaatgccc
      241 acgcgatcag cgtcgggatc ggtgccgatc acaaggtcgg cccccacctt tttcgccaga
      301 gccacggcca tgtccagggc cgccccctcc tcggggttgg gtgacttgac ggtaggaaaa
      361 ccgccgtcgg gtgcggcttg ttcgggaact tcgatcacct gtttgaagcc gcggcgacgc
      421 agggcctcgg gtgtcagcac gctgccggta ccgtgcagac cggtaaagac gatcttgagc
      481 ccttcgccct gccggcgaca gacctgggga ttcaggcacg acgtctgtac cgcctgcagg
      541 aacgcctcgt caatttccct gccgatgatc ttgagaagtc ccttggcctt cgcctcggcc
      601 ggggacatga ccttcacgtt tccaaagccg ccgactctgg ccacctcggc catgatgttc
      661 ttgtcgtgcg gcggaaccac tgagacgccg tcggaccagt aggctttgaa tccgttgtac
      721 tgcggtggat tgtggctggc ggtgatgacc acgccggccg tgcacccgag gtgacggact
      781 gcaaaggaca gctcgggcgt cggtcgaagt cgatcgaaca gatacgccgg gatgtcgtta
      841 gcggccatca cgcaggccac ccgctcggca aagatgtcgc tcatgcggcg gcaatcatag
      901 gcgacgacca caccggcttt gcgggcggcg tcgccctggc tccgaacgta gttcgccaat
      961 ccttgagccg cggcaccgac cgtgtatatg ttcatccggt tgagacccgc gccgatcacg
     1021 ccgcgcatgc cgccggtgcc gaactcgaga ccgcgataga agcggtcggt taactccctt
     1081 tcgttgttca cggccaacag gtcgcgaatt tccttgcgat cggcctcggc aatggccgga
     1141 tcgttgatcc acgaatcaac agcattcttc accgattggt ccattacgtg cagcctcgaa
     1201 gagaagtcgt ttagtctctt ggtctgttag tctattagtc tgttaggtcc attggtctct
     1261 ttcgtcgtga ctggtgtgcg ttttggctgc gggccgcaac ccaaacgttc tgcgatccag
     1321 aaacatgcgc gcagaatgcg caccggctgc aacggaagag actaaaggtc tagcagccta
     1381 cggtcctcct gaagtactcg atcgtccttc ggatgccctc ttccaacggt actttcggct
     1441 catagccgag caacgtcctc gccttggtca ggtccgggcg acgacggcag ggatcatcct
     1501 gcgggagagg cctgtactcg acccgcaagg accgccccat cgccttttcg atcgcctcga
     1561 ccagttcgag caaggtgatc tcggagtcgt tgcccaggtt gatcggaccg atgtgctcgc
     1621 tttccatcag gcggcagagc ccatctacca gatccgacac ataacagaag ctccgggtct
     1681 gcttgccgct gccgtaaacg gttatcggtt cgccgcgcag ggcctgacaa atgaagttac
     1741 tcatcacccg cccgtcgtcc acggccatgt tgggcccata ggtgttgaag atgcgggcga
     1801 tgcggatgtc gaccttgttc tggcggtggt agtccatcat cagggtctcg gctacgcgtt
     1861 tgccctcgtc gtaacaactg cggatgccga tcgggttgac gtacccccaa taagtctcag
     1921 cttgcggatg aacctgcgga tcgccgtaga cctccgaggt gctggcctga aggattcgag
     1981 cccgaacccg tttggccagg ccgagcatgt ggtacgtgcc gaggacattg gtcttgatcg
     2041 tcttgacggg gttgtactgg taatggaccg ggctggccgg gcaggccaga tggtaaatct
     2101 tatctacctc cacgacgatc ggctcaatca cgtcgtggcg aagaaactcg aaccggccgc
     2161 tgagcaccag ctcctgataa cgatcgccaa agttctcaag tcgaccggtg aagtagttgt
     2221 ccaggcaaac gacatggtgt ccctcgtcaa acagacgaag gcacaggtgg gaaccgatga
     2281 aaccggctcc gccggtaacc agaataacat ccttgtccat tcgccaacct ccgggcagcc
     2341 acacttgtga aacgtcgcgc ctgcaattcg acccggcagc atgatagctg cagtattgga
     2401 gcgcgtcgag gtattatcgg acagtcgcgc gacggggttg agatgggctg gagagcatga
     2461 attgactgat ggaactccgt ctgagccaag cgcccgtccc gaatcgccgg tttcttggca
     2521 gcgaggcaac tcatagcagg gtaagtgctc gattttcaca gcggcatgct tgaccggatg
     2581 gggcggtgat atcctaaact agttgagtct ttgcccggtt gtagaccgct gcgactgact
     2641 tgtgtctact cggaccttcg ggcaatctgg acgggaatgg atgacgccat gggattggtc
     2701 aagaccgtct ttcacattca cacggactac tcggatgaca gcaatctgtc cgtggattgt
     2761 ctggccgacg aagccatgga aaggggcgtc ggctgcgtgg caatcaccga ccacgattcg
     2821 atcgaggggg cgatgacgct ggcagatagc gtgggcgacg atctccaggt catcgttggc
     2881 gaggagattt cgaccaagga cgggcatttg atcgggctgt tcttgcgcga cttggtggag
     2941 ccggggatgt cggtgcggca gacggccgag gccatccgcg agcagggagg cctggtggtc
     3001 gttccacatc cttacaacag gctttttcat tgcagcctgg gcgaccgggt ttacgacatt
     3061 ctcgacctga tcgatgcggt ggaagtcgcc aatgcacaga atctctcgcc gctgcccaac
     3121 aggcgatcgg cgaggttcgc ccgccgtcac ggttttccgg ccgtcgtggg aaccgacgtg
     3181 caccatcgag gctacctgga cacctgctat cagataatgc cgggttttga ggggccaagc
     3241 gggtttgtga cctcgctggg gcgtgcccgg ttgcatgtcc gttcacatcc gctgagctac
     3301 ttcctccgga ccgcctggtt gattacgggc ggcaagctgg ggctgcctct cccggccggc
     3361 tatggcgcaa acgccggcga agtgcccgtg aggtcatcga cgcggcaggc ggttctgtcg
     3421 gcggagtgac cccagaagca ctcagctttc agcggtcagc gttcacccat tagcccccgt
     3481 cttgtcaagc gcgaaaactc ccttggcgaa
//
LOCUS       DAOTPW010000379         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_21203_length_3502_cov_4.712794, whole genome shotgun sequence.
ACCESSION   DAOTPW010000379 DAOTPW010000000
VERSION     DAOTPW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21203_length_3502_cov_4.712794"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..1680
                     /locus_tag="P5316_21145"
     CDS             337..1680
                     /locus_tag="P5316_21145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87520.1"
                     /translation="MPAYVSFRWRSSRFALSAFFAVMPAANAAESPEESPGQGSTRPA
                     TVPAGLSDDLTVWPNKVSYRNSDPWIWQNHDRIREMRPRVLVLNFANDVDMPGIQERT
                     DKLIKALAESTRYHGFKDPDAPAFLRYEVAKYVDMRDRPVPPERLHKNSAFLPLKPDG
                     PKDFGCDYGTFYGDAFARFYGFQDPRNDRRYLNLHELINAGVVHELWFYAIHEYEVGW
                     PAFEVTEFKQYYDDQCRPIEGKHGPAGNGHDDSMPWSGRSFRMAFFNPHRGTGCQMEN
                     FGHGLEAYANHNAIGYWRKYFREFAELNLDERFKLPFSSLYEMPYGRDNVVSYPTATT
                     MKIKHHDQEYTVEPYIAVGGNAHWPPGARHHYDLDSSYTVKSTIENYRLRNGPDGKDK
                     VLDFNKEKYAPYRKTVPDCMGAWMVFWRQCMPGLDNKCVDDDGKPMKNWWVFLFY"
     gene            1712..>3502
                     /locus_tag="P5316_21150"
     CDS             1712..>3502
                     /locus_tag="P5316_21150"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRR87521.1"
                     /translation="MNRLQSYLTPVALVLSFSVSAIAAETIALSEDNMEITKDTVVKP
                     GRYRLPDPGDDGAVRIIGNDITVDFQGAEIDGSSEGRLPDAFTGRGIIIRGRNITLRN
                     AKVRGYKVGIYANECPGLLIEDVDVSGNYQKHLLSTPEAEDDSDWIFGHENDKKEWIA
                     NYGAALYVELSDKITLRRVKARHGQNGICIDRVNDSKIYDNDCSFLSGWGLAMWRSNR
                     NVITRNAFDFCVRGYSHGVYNRGQDSAGIFMFEQNCENVLAENSATHGGDCFFGFAGL
                     EALGDKPAPSPDFDYRRRGNNDNLLINNDFSYAPAHGIEMTFSFGNKFIGNRLVGNAI
                     CGVWGGYSQDTLIAGNLFEENGGMGYGLERGGVNIEHGSANVIKHNTFRNNRCGVHLW
                     CAPNPDFMQKPWGKANHQNLKPTTIAWNTFEGDEVGFHLRHAIETILIDNRMTGVKRE
                     MVADENAKTTRLDQPAEKWEPPKYPVFGDTRPVGARKKLAGRHNIIMTEWGPYDFTDF
                     IVYPRKVLAGEKATVQILGPSGDFKVTKVTGSVHVTPMEGRLPGMVTVSAVKKGVLPF
                     SIELDAGGKKLSAVGTLVSADWDVKFYKWDP"
BASE COUNT          752 a         1040 c         1052 g          658 t
ORIGIN      
        1 gcgagggatg acagaagggc tggaagcttg ctgaggcccc ggctcgggct gaaggggcag
       61 acgagtcgaa tcgggcttct aacccttgtt ttgaaggtaa gttacggctt tcagtttccg
      121 gctggaatgg gatcttgcct gcctggggcg tactccggag ccagccagcg cccggacaag
      181 ccttgtggta tcagctttgg tcaatccccc ggacaatcag agcttggaag cggcttgact
      241 tccgtgtacc ctggccaacc cccaagcgct catagggcaa acgcggtgct cctcgcaggc
      301 ccaagagttg gcaggagacg ggaggacaag cagagcatgc cagcctacgt gtcgtttcgg
      361 tggcgttcgt cgcggttcgc gctcagtgcc ttcttcgcgg tgatgcctgc cgcgaatgcc
      421 gctgagtcgc ccgaggagtc tcccggccaa ggctcgaccc ggccggccac ggtaccggca
      481 ggcctttcgg acgacttgac cgtctggccc aataaggtca gctatcgcaa ctcggacccg
      541 tggatctggc agaatcatga tcggattcgc gaaatgcggc cgcgcgtgtt ggtgctcaac
      601 ttcgccaacg acgtggatat gcccggcatt caggagcgta ccgataagct catcaaggcc
      661 ctggccgaga gcacacgcta ccacggcttc aaggaccctg acgccccggc attcctgagg
      721 tatgaggtcg ccaagtacgt cgatatgcgc gaccggcccg tgccacccga gcgtctacac
      781 aagaacagcg ccttcctgcc gctcaagccc gatggcccca aggatttcgg gtgcgattac
      841 ggcacgttct atggggacgc ctttgcccgc ttctatgggt tccaggatcc gaggaacgac
      901 cggcggtact tgaacctgca tgagctgatc aacgcggggg tcgtgcacga gctgtggttc
      961 tatgcgatcc atgagtacga ggtgggctgg ccggcgtttg aggtcaccga gttcaagcag
     1021 tattacgatg accagtgccg gccgatcgag ggcaagcacg gtcccgccgg caacgggcat
     1081 gatgattcga tgccgtggtc cggccgcagc ttccgcatgg ccttcttcaa cccgcaccgc
     1141 ggtacaggct gccagatgga aaacttcggc cacggtctcg aggcctacgc gaatcacaac
     1201 gccatcggct attggcggaa gtacttccgg gagttcgcgg aactcaatct ggatgagcgg
     1261 tttaagctgc cgttcagctc actgtatgaa atgccctacg gccgcgacaa cgtcgtctct
     1321 tacccgacgg cgacgaccat gaagatcaag caccacgatc aggaatacac ggtggagccc
     1381 tacatcgccg tcgggggcaa tgctcactgg ccgcccggag cccgccacca ttacgatctg
     1441 gacagctcgt acaccgtcaa aagcaccatc gagaactacc ggctgcgcaa cggcccggac
     1501 ggcaaggaca aggtgctcga tttcaacaag gagaaatacg ccccataccg caagaccgtt
     1561 cccgactgca tgggcgcgtg gatggtcttc tggcggcagt gtatgccggg gttggacaat
     1621 aagtgcgtcg atgacgacgg caaacccatg aagaactggt gggtgtttct gttctactga
     1681 tgtcgaaccg caaatgatga ggacccgtcc tatgaaccgg cttcaatcat atctgacccc
     1741 ggtagcgctc gtgctttctt tctcggtctc ggccatcgcg gctgaaacga tcgccctgag
     1801 cgaggataac atggagatta cgaaagatac ggtcgtcaag cctggccgct atcgcttgcc
     1861 cgatcccggc gacgacggcg ccgtgcgcat catcggaaac gacattacgg tcgacttcca
     1921 gggagccgag atcgacggct cctcggaagg acggcttccc gacgccttca ccggcagggg
     1981 aatcatcatc cgtggccgga acatcaccct cagaaacgca aaggttcgcg gttacaaggt
     2041 gggtatttac gcgaacgagt gtccggggct tctcatcgaa gacgtcgacg tttccggcaa
     2101 ctatcagaag catctgttat ctacgccgga ggccgaggac gacagcgatt ggatattcgg
     2161 gcatgagaac gacaagaagg aatggatagc caactatgga gccgccctgt acgtcgagct
     2221 gtccgacaag atcacgcttc gccgcgtgaa agcccgtcac ggccagaacg gcatctgcat
     2281 cgaccgcgtc aacgattcca agatctacga caatgactgc tcgttcctgt ccggttgggg
     2341 cctggccatg tggcgatcca accgcaacgt gatcacgcgg aacgccttcg acttctgtgt
     2401 tcggggatac agccatggcg tttacaaccg cgggcaggat tcggcgggca tcttcatgtt
     2461 tgagcagaac tgcgaaaacg tcctcgcgga aaactccgcg acccacggcg gcgactgctt
     2521 cttcggtttc gccggcctgg aggccctcgg agacaagccg gcgccgagcc ccgacttcga
     2581 ttacaggcgg cggggcaaca acgacaacct gctgatcaac aacgattttt cctacgctcc
     2641 ggcccacggc atcgaaatga ccttcagctt cggcaacaag ttcatcggca accggctggt
     2701 gggcaacgcc atctgcggcg tctggggcgg ctactcgcag gacacgctga tcgctggcaa
     2761 tcttttcgag gaaaacggag gaatgggcta cggtctggaa agaggcggcg tcaacatcga
     2821 gcacggctcg gcgaacgtca tcaagcacaa caccttcaga aacaaccggt gcggcgtgca
     2881 cctttggtgt gccccgaatc cggatttcat gcagaaaccc tggggcaagg cgaaccacca
     2941 gaatctcaaa cccacgacga ttgcctggaa cacgttcgag ggtgatgagg tcggctttca
     3001 tctgcgccac gcaatcgaga cgattctcat cgacaatcgg atgaccggcg tgaaacggga
     3061 aatggtcgcg gatgagaacg ccaagaccac cagactcgat cagccggccg aaaaatggga
     3121 gcctccgaag tatccggtct tcggtgacac ccgaccggtc ggtgcccgca agaaactggc
     3181 tgggcgacac aacatcatca tgaccgagtg gggaccgtac gatttcaccg acttcatcgt
     3241 ctatcctcgg aaggtcctgg ccggtgagaa ggctactgtg cagattctcg gtccctccgg
     3301 cgacttcaag gtcaccaagg ttaccggctc ggttcacgtg acgcccatgg agggcagact
     3361 tcccgggatg gtcacggtct ctgctgtgaa gaagggcgtg ctgcctttct ccatcgaatt
     3421 ggatgcgggt ggcaagaagc tctcagccgt cgggacgctg gtgtccgcgg actgggacgt
     3481 caagttctac aaatgggatc cc
//
LOCUS       DAOTPW010000380         3458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_21506_length_3458_cov_6.980018, whole genome shotgun sequence.
ACCESSION   DAOTPW010000380 DAOTPW010000000
VERSION     DAOTPW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21506_length_3458_cov_6.980018"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="P5316_21155"
     CDS             <1..1363
                     /locus_tag="P5316_21155"
                     /inference="COORDINATES: protein motif:HMM:NF033662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87522.1"
                     /translation="SHRSAGASAGRYRGLNGYITCNLPITGAAMGKWNLVVTKTNCNE
                     AQLNNAIIVAYPTLTNGSFESPLGFASSCPNPPTPIVAPTDWLQLGVSTGSDHLIRDS
                     DQWPPSCPRPDGDHFASVMVPTATSFGHWRVFQYIAVTPGQTITVSGRFAGGGRSASR
                     LQILEGDDYVDNDAELLASTLIEDRVGCPPHSYDWVPVSVTATPTGGLITVRWLINGH
                     TSVPVAAHADALTLTIGDPPAEVCGNGIDDDGDRRTDCQDPDCATAPGCDPPPVEICD
                     NGIDDDGDVYIDCDDSDCDSVCVEICNNGIDDDHDCDVDLDDSECENCTNGVDDNGDG
                     LVDCDDPTCLGHEACPSEICTDGIDNNGDTLIDCADPGCAQHPHCKANDPFADYDGDG
                     DVDQNDFAALQVCVTGAGGTLDIGCGMFDRDFDLDIDQTDINRFEMCASGPGVPAAPA
                     CDN"
     gene            complement(1598..2377)
                     /locus_tag="P5316_21160"
     CDS             complement(1598..2377)
                     /locus_tag="P5316_21160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR87523.1"
                     /translation="MDTTHSRRGFTLLEILVATAVILLLIAILLSSLEQSQTRAKSTV
                     CLANLRSIGTAMRLYQEDARGWLPVGPADKVWYVDRMIGLVREPAPGRRPYPWTNCHW
                     GGKRAAWIHTIRNDPPVPETLGRPLTRYLYRGTGLDQPTPLFECPSDVGSPLLENPIG
                     EKPIYYLCGNSYYTNPWESYQPLGTKRSPPSAVVLVEEGAMYMDLVSGRQNLGWHGRF
                     STHNALFLDFHAETRFMDTRSYHGPGWVVENYFDIMDYYRY"
     gene            complement(2662..3213)
                     /locus_tag="P5316_21165"
     CDS             complement(2662..3213)
                     /locus_tag="P5316_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87524.1"
                     /translation="MSLFHMRHARSIIGMALLLSVAGCSPMSAAVSGAKVSMKVFTGA
                     QAKAHPLEGLSPDALQPYRSIALGEVTTDVSPICTFDVLSEVRSALAEAFADEHLRRH
                     FPGGKPRLVVNVVVRFFKQGNLFGKEPRLDLLVSFVDGSDGREVGRVYVEGISESPLE
                     TKARHLAAADAKELANLLRSRKK"
BASE COUNT          671 a          992 c         1086 g          709 t
ORIGIN      
        1 atcgcaccgg tcagccggcg ccagcgctgg tcgctatagg ggtctgaacg gttacatcac
       61 gtgcaatttg cccattaccg gcgcagccat ggggaaatgg aatcttgtcg tcacgaagac
      121 caactgcaat gaggctcagt tgaataacgc cattattgtc gcgtatccga ctttgactaa
      181 tggaagcttc gagagtccgc tcgggtttgc cagcagttgt ccgaatccgc cgacgccgat
      241 cgtcgctccc accgactggc tgcaactggg cgtttcaacc ggcagcgatc atctgattcg
      301 ggattcagat cagtggccgc catcgtgtcc tcgcccggac ggtgaccatt ttgcttctgt
      361 tatggttccg accgcgacct cattcgggca ttggcgcgtc ttccagtaca tcgcggtcac
      421 tccgggccag acaatcacag tgagtggtcg ttttgcgggc ggcggtcgat ctgcttcccg
      481 tctgcaaatc ctggaaggtg acgactacgt tgacaatgac gccgagcttc tcgcgtcgac
      541 gttgattgaa gaccgggtgg gttgcccgcc gcattcgtat gactgggtgc cggtttcggt
      601 cacggccacg ccgacgggcg gtcttatcac ggttcgatgg ttgatcaatg ggcacacctc
      661 ggtaccggtt gcggcgcacg ccgatgcgtt gaccctgacg attggtgatc cgcctgccga
      721 ggtctgtggc aacggaatcg acgatgacgg tgaccggcgc acggattgtc aagatccgga
      781 ttgtgcaacc gcgccgggct gtgatccgcc acccgttgag atctgcgaca acggaatcga
      841 tgatgacggg gacgtctaca tcgactgcga cgacagcgat tgcgattccg tttgcgtcga
      901 gatctgcaac aacggaatcg atgacgacca cgactgtgac gtggacctcg atgacagcga
      961 atgcgagaac tgcaccaacg gagtggatga taatggcgac gggctcgtcg actgcgacga
     1021 cccgacgtgc cttggacacg aggcatgccc gtccgagatc tgcaccgacg gtatcgacaa
     1081 caacggtgac accctgatcg actgtgccga cccgggctgc gcccagcatc cacactgcaa
     1141 ggccaacgat ccgtttgccg actacgacgg cgatggggac gtggatcaga atgattttgc
     1201 ggcattgcag gtttgcgtga ccggtgccgg tggaacgctg gatatcggct gcggcatgtt
     1261 cgaccgcgat ttcgatttgg atatcgatca aactgatatc aacaggttcg agatgtgtgc
     1321 cagcgggccc ggagtgccgg cggcgccggc gtgtgacaat tgacagttcc ctggcgggac
     1381 cggctcattc gattgtctga caagagaccg gcggggtcga ttaccctgcc ggtctttttt
     1441 ccggtacgtg tgggcacgtc acaggtgagc gtgaattggt tttccgggat atccctcgca
     1501 gcgtccttgt cgggcaacga catgaaggac gtttcattct caaccctgcg ttggcgggtt
     1561 ctctgcttac gcgtgccgtg gcgggcgaaa gccgggatca ataccggtag tagtccatga
     1621 tgtcgaagta gttctcgacg acccagccgg gaccgtgata gctgcgggta tccatgaatc
     1681 tggtttcggc atggaagtca aggaataggg cgttgtgggt agagaaccgg ccgtgccagc
     1741 cgaggttctg tcgaccgcta acgagatcca tgtacatcgc gccctcctcc acgagcacaa
     1801 ccgcggacgg cggagaacgc ttggtgccaa gcggttgata actctcccac gggttcgtgt
     1861 aatagctgtt gccgcacagg tagtaaatgg gcttttcgcc gatggggttt tccagcaaag
     1921 ggctgcccac gtcactgggg cattcgaaca acggagtcgg ctgatccagc ccggtgcccc
     1981 ggtagagata tcgggtcagc ggccgtccca gggtttcggg tacgggcggg tcgttccgaa
     2041 tggtgtggat ccaggcggct cgcttgcccc cccagtggca gtttgtccac ggatagggcc
     2101 ttcggccggg agccggctcg cggaccaggc cgatcattct gtcaacatac cagaccttgt
     2161 cggcgggacc gaccggcagc cagccacgtg catcctcctg gtagagcctc atggccgttc
     2221 caatggagcg caagttggcc agacagacag tcgatttggc ccgagtttgg gactgttcga
     2281 gcgaagacaa caggatggct atcagcaaaa ggatgactgc ggtcgcaacc aggatctcga
     2341 gaagtgtgaa acctcgacgg ctgtgagttg tgtccatcgt ccactccgct gaaggctcgg
     2401 gcagatcgcg aaatggaact tacctcaagc gccctcagca tagcaatcgg ctgtctgcaa
     2461 gggaagaggg cacctgccag tatttcggca tttccactct cgcagcccag cggtttcacg
     2521 cgggggcggg aggggaggta atcgcccgcc tgggttgccg gtggctcggc ggtcgggggc
     2581 tgggcaggag cgggcgcatt gcaggctacc gcctgcccgg cggggcccgg gcgaagtgca
     2641 atcacttgac tttttccaaa attatttctt ccggcttcgg agcaggttgg caagttcctt
     2701 ggcgtcggcg gcggccaggt gtctggcttt ggtttccagc ggcgactcgc tgatcccctc
     2761 gacatagaca cggccgactt cacggccgtc gctcccatcg acgaaactga cgagcaagtc
     2821 cagacgaggc tctttgccga acaggttgcc ttgcttgaag aatcggacga ccacattgac
     2881 aaccagtcgg ggcttgccac cggggaaatg gcgacgaagg tgttcgtcgg cgaacgcctc
     2941 tgccagggcg gaccgcacct cggacagaac gtcgaacgtg cagatcgggc tgacgtccgt
     3001 cgttacctcc ccgagtgcga tggaccggta aggttgaagg gcatccggcg agagcccctc
     3061 caacggatgg gctttcgcct gagctccggt gaagaccttc atgctcacct ttgctcccga
     3121 aaccgcggcg ctcatcgggc tgcaccctgc gacgctgaga agcagcgcca tgccgattat
     3181 cgaccgggca tgtcgcatat ggaacagact catgatgtcg caacctcccg aggcggaccg
     3241 cctttttgcg ggccgcaacc gaagcgcact tgagtccgga aacaagcgcg caagccgcat
     3301 agctttccag ccctgaggct ccattcattg ctcgagggct tttgaaatcg ctctctgatg
     3361 ccctcgcaga ccgattgcag tttactcaac tccgaggcgt ggcaaaagag gccgggcggt
     3421 cggtttctga agagatagtg gctgaccagc cagatttg
//
LOCUS       DAOTPW010000381         3428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_21759_length_3428_cov_5.777646, whole genome shotgun sequence.
ACCESSION   DAOTPW010000381 DAOTPW010000000
VERSION     DAOTPW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21759_length_3428_cov_5.777646"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(915..>3428)
                     /locus_tag="P5316_21170"
     CDS             complement(915..>3428)
                     /locus_tag="P5316_21170"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRR87525.1"
                     /translation="DFSAHAGVNAVIVAPRINERTAAAAGEWLVDDVEVISATSGPRP
                     VIEALYMPRPILQLNDSAELIVQVANQGALPLKPSRIRFGSVNAPATAAADIAASGNE
                     TGRSAQTSDTGDQPPLEIRSKFGDRITELPIPALSPDQSLRMSLKVTGKRKGSVEIKA
                     EWVGEGFSVVRTRRTVCVDRPNPRELCTGVGGVWRFMPTPVPLQQNSDATMEALPTVK
                     SSELPDSCIGVAAHLPRSRDFEVVFEPEHLIDGKPETSWSGRAHATPVPGSSEWVEVY
                     FSATSAIKELRLLPYWRAEGFPADFDIKVRDRGSWKTVQQIRHASLPPSAGEGEKHPY
                     AITFPRPVQADAICIEATRFTPTSGFFTDCGTTYYFRLSEIEAINTDDKNVAAAAAGA
                     GAKASWTFRSYFNSAEVIRDTYAELYNLGVKWNRIGQWGDWTCWSIVEQKKGEYAIDP
                     TTDQAITESVSNGVNIVYCLCYGNALYEETPPHANLGPLWRHGHPFMGCAGPTKPESI
                     EAFVNYARFVANHFRGRVKWYEIWNEPNSWAWYGSPPDPKAFGTLLRETAKAIKEVDP
                     DCGVLVGGTAALAPVFLSKALAEGGGPHLDAVAFHPYGAPFPEAGAGSLDIIDGKQVS
                     RSPADLGFSTNREMLDFLRRTFSPFNPHFDFWANEWNAIPTREDSPHQGGTEITEAKQ
                     AARFFLQGTLTGVRSIWWSLVNENTVYDWGILRTGELSRKPVYYTIQAMTTLLSGAHA
                     DPTIKATATGDAPELRCETLRGRDGEMLVAIWSVISPQDDYAGKRVSVTIANAAGKSV
                     DAVDTFHALVQKIEAKTDGDAMVIEGLLAMDYPVILRIR"
BASE COUNT          629 a         1000 c         1077 g          722 t
ORIGIN      
        1 aatcgcgcaa tcggcgtcgc cgatcggtgg gtcgccttcg gtgtccacga ttgcctgtga
       61 caggtcgatc agatagcact tctggtcgag caggaagtca tcgtcgacca tcggatcatc
      121 gatgaacgtg atgacgactc tggtattgcc gctatcgaga tagacgtcgt tcggggtgat
      181 gaagcgaaat gcacggggtc aattcttgaa atgtacgggg tcaactgaca cattccggtc
      241 aatgcacggg gcaatgtacg aggtcaactc tcgactcctg acacattcct gtccaactcc
      301 tgcatcgttt gttgcctgac caattcatcg tgccaggcaa cctccctccg caaagtcaag
      361 cgccggggct gtgtcaagcg aagtccgccc accgctgctc tctgtgatct ctatgcccac
      421 gtggtgaacc tgcacgaacc gccaacccgc caagcagaca tctgcgggca caccggcgta
      481 ccggctacgc tttcgcgtat ttggcgtgct tcacggttcg aacttcgccc aaatgtcaag
      541 agtcaagagt tgaccccctt atcgcatttg gtcaggatac ttctgtctcg cccaaaaggg
      601 ctccgcacct cccgtcctgt cgccgacagc cacgctcaag ggcagtgcgg tttcactgac
      661 ccaaccctgt ctcggatgaa cgttcttgct catacccgcc tctcgccatg caggcgggcg
      721 tggttttctt cgtgcatatc ccaaggaaag aaacgagtta tgaaaggtag cccagttgaa
      781 agccagcttg aacggctggc cgggtgtccg gcgcaaactg gacatgccat tccacccgct
      841 cgaacgctgg agcaacccgt ctcggcccgg cagtttaagg gcacacgcct cgctcgcagg
      901 agaagtgcat agtctcacct gattcggagg atgaccgggt agtccattgc cagcagtccc
      961 tcgatgacca tagcgtcgcc gtcggtcttg gcctcgattt tctggaccaa ggcgtggaag
     1021 gtgtcgaccg cgtcgaccga cttgccggcc gcattggcga ttgtgacgct gacgcgcttg
     1081 ccggcgtagt cgtcttgcgg gctgataacc gaccagatgg cgacgagcat ttcgccgtcg
     1141 cggccgcgca aggtttcgca acgcaattcc ggggcgtcgc ccgtggccgt tgccttgatt
     1201 gtcgggtcgg cgtgggctcc gctgagcagt gtggtcatgg cctggatcgt gtagtagacg
     1261 ggtttgcggc tcagttcgcc ggttctgagg attccccagt cgtagacggt gttctcgttg
     1321 accagcgacc accaaatgct gcgaacaccg gtcagcgtgc cctggaggaa gaacctggcg
     1381 gcctgcttag cctcggtaat ttcggtgccg ccctggtgag gggagtcttc ccgagttgga
     1441 atggcattcc actcgttggc ccagaaatcg aagtgcgggt tgaaaggcga gaacgtccgt
     1501 ctcaggaaat cgagcatttc gcgattggtg ctgaagccaa ggtcggcggg ggaacggctg
     1561 acctgctttc catcaatgat atcgagcgat cccgcgccgg cctctgggaa aggggctccg
     1621 tagggatgga acgcgaccgc gtcgaggtgc gggcctccgc cttcggcgag agcctttgag
     1681 agaaagacgg gggccaaggc ggccgtgccc cccaccagga cgccgcagtc gggatcgact
     1741 tccttgatgg ctttggccgt ctcgcggagc aatgtgccga aggctttcgg gtccggcggg
     1801 ctgccgtacc aagcccaact gttcggctcg ttccagatct cgtaccactt gacgcgcccg
     1861 cggaagtggt tggccacgaa gcgtgcgtaa ttcacgaatg cctcaatgga ttccggtttt
     1921 gtcggcccgg cgcagcccat gaaaggatgc ccatgtcgcc agagcgggcc gaggtttgcg
     1981 tgcggcggag tctcttcgta gagggcattt ccgtaacaca ggcaatagac gatgttcacg
     2041 ccgttgctga cggactctgt gattgcctgg tcggtggtcg ggtcgatggc gtactcaccc
     2101 ttcttctgct cgacgatgga ccagcaggtc cagtctcccc actgtccgat gcgattccac
     2161 tttacgccga ggttgtagag ttcggcgtag gtatcgcgta tcacctcggc ggagttgaaa
     2221 taggaccgga aggtccagga ggccttggcg ccggcacccg cggcggcggc ggcgacgttc
     2281 ttgtcatcgg tattgatggc ctcgatttcc gacaggcgga agtagtacgt ggttccgcaa
     2341 tcggtgaaga aaccggaggt cggggtaaag cgggtggctt cgatgcaaat cgcatcggcc
     2401 tgcacgggtc gcggaaacgt gatggcataa ggatgttttt cgccctcgcc ggccgacggc
     2461 ggcagactgg cgtgcctgat ctgctgaacc gttttccatg agccccgatc gcgcaccttg
     2521 atgtcgaaat cggccgggaa gccctctgcc cgccaataag gcaggaggcg gagctcctta
     2581 attgcggacg tggcggagaa gtacacttcg acccactccg acgatccggg cacgggcgtg
     2641 gcgtgagccc ttccggacca gctcgtctca ggcttgccgt cgatgaggtg ttcgggctcg
     2701 aatacgactt cgaaatcgcg cgatcgcggc aggtgcgcgg cgactccgat gcagctatca
     2761 ggcagctctg atgatttcac ggttggcagg gcttccatgg tcgcgtcgct gttctgctga
     2821 agcggtacgg gtgtgggcat gaatcgccat acgcctccga cgccggtgca cagctcgcgt
     2881 ggattggggc ggtcaacaca tacggttcgg cgtgtcctga cgacggaaaa gccctcgcca
     2941 acccattcgg ccttaatctc gacactgcct tttcgtttcc ctgtcacctt cagagacatt
     3001 cgcagggact ggtcgggtga tagggctggg ataggcagct ctgtaattcg atctccgaat
     3061 tttgatcgaa tctctaacgg cggctgatcg cccgtatcgc tcgtttgtgc ggatcggccg
     3121 gtttcgttgc cggatgccgc gatgtctgcg gcagcggtcg cgggagcgtt taccgaaccg
     3181 aagcgaatcc gggagggttt gagcggcaga gcgccttggt tggcgacctg cacgatcaac
     3241 tcggctgagt cgttgagctg caagatgggc cggggcatgt aaagagcctc aataaccggc
     3301 cgtggaccgg atgtggccga gatgacctcg acgtcgtcca ccaaccactc gccggcagcc
     3361 gccgcggtcc gttcgttgat gcgaggcgcg acgatcactg cattcacacc ggcgtgagcg
     3421 ctgaagtc
//
LOCUS       DAOTPW010000382         3421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_21823_length_3421_cov_5.654189, whole genome shotgun sequence.
ACCESSION   DAOTPW010000382 DAOTPW010000000
VERSION     DAOTPW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21823_length_3421_cov_5.654189"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1572)
                     /locus_tag="P5316_21175"
     CDS             complement(145..1572)
                     /locus_tag="P5316_21175"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR87526.1"
                     /translation="MRAISLICLWVVTPVIPAAAGPADHERVAPSSRRPNVVIVFPDQ
                     LRASEVGCYGHAVVRTPSIDRLAATGVRMEFAFSNFPVCSPARSILLSGRYARSTGVL
                     RNQDNEAGPGRPTNQTPTLAEVLAANGYDTVLIGKWHLAPTPQALGFRESLRPRMRHR
                     YFKQTFFRNEGEPYIYDDYAPYHETEAAVEYIRGHKDRPFFLYLAYGPPHMPVSEMPE
                     QYRRMYDPAKVVIRDNTWVNGRLAYDENWFKIYMWDFQYYEHKDTFGQPLPAGMTLRE
                     LTALYDGQITAVDECIGRVLDAIRQAGIEDDTIVLLTSDHGDLLGSHGLFNKNTHHEE
                     SIHIPMIFRYPQRLKPAVVDAQIVSLVDVMPTLLDLCGLAAPDTVQGTSIAPVLLGRR
                     KTVGENVALIETSSADGVRALRHVYFNDRKKGTEHLFDVAADPFELKDLAKDPASREV
                     LEALRARVKEWNVRCPRWPTASSRN"
     gene            1874..3364
                     /locus_tag="P5316_21180"
     CDS             1874..3364
                     /locus_tag="P5316_21180"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRR87527.1"
                     /translation="MKRWLTTLVIVAQTVCLLPAGPAHADDVRKSVVKVFSTKSPPNM
                     FRPWEITPPQEVTGSGVIIEGGRVLTSAHVVSWSQQIYVQPNESSEKLDATVEYIAED
                     CDLAILTIDDEDAIKDIKPVPLAEKLPPPKTKVNVLGYPVGGDTLSVTEGVVSRIEWT
                     NYNYGTAALRIQVDAAINPGNSGGPAIVQDAVAGIVFSRFPEGENIGYLIPAEVVRHF
                     IDDFKTDGKYDGFPKIDVRTATLENPSLRSYLNIERQQTGVVVHRVDQPDLKEKIKPW
                     DIISACDGVEIDNLGVVPIAEDIRVEWGYLISKKAPGSTVKLKLIRQGKPAEVEVATI
                     TKDNGIIQRMTRTRPTYFLYGGLVFSPATAELVSVIPPRYVAMLGSRGSLLFKRADED
                     RVEPGDEIVVACSGILPHRITKGYGISPLSVLTHVNDQPVKSLRHAIELIKAGKDKDY
                     VVFRFEEENEAKIVLEPKLVEKFTPDILRNNNIPSDRSEDLKDVWP"
BASE COUNT          721 a         1113 c          966 g          621 t
ORIGIN      
        1 cgggtgccat gcccacggct ttgcgtgggc atgcgccttt ccatccttac ggccacacat
       61 ctgtgcggct tgcgcgcatg tttccggatc gcagaacgtt tgggttgcag cccgcaaagg
      121 cggtccggct cggcagcttg atggtcagtt cctacttgag gccgtcggcc atctcggaca
      181 ccggacgttc cactccttca cccgggcccg aagcgcctcc agcacttcgc ggctggccgg
      241 gtctttggcc agatccttca actcgaacgg atcggccgct acgtcaaaca ggtgctcggt
      301 gcccttcttg cgatcgttaa agtacacgtg cctcagcgct cggacgccgt cggcggagct
      361 ggtctcgatg agggcaacgt tttcgccgac cgtcttgcgc cgccccaaca gaaccggggc
      421 tatcgacgtg ccctgtacgg tatccggagc cgccagcccg cacagatcca gcagcgtggg
      481 catcacgtcc accaggctca cgatctgtgc atcaaccacc gccggcttga gccgctgcgg
      541 atagcggaag atcatcggga tgtggatcga ttcctcgtgg tgcgtgttct tgttgaaaag
      601 gccgtggctg cccagcaggt cgccgtgatc ggaggtcagc agcacaatcg tgtcgtcctc
      661 gatcccggcc tgacgaatcg catccagcac ccggccgatg cactcatcca ccgccgtgat
      721 ctgaccgtca tacagggcgg tcagttcacg aagggtcatc ccggccggca atggctgccc
      781 gaatgtgtcc ttgtgctcat aatactgaaa atcccacatg tagatcttga accagttctc
      841 gtcgtaggcc agtctgccgt tgacccaggt gttgtcgcgg atcacaacct tggccggatc
      901 gtacatcctg cggtattgct ccggcatctc cgaaacgggc atgtgcggcg gaccatacgc
      961 caggtacaga aaaaacgggc gatccttgtg cccgcggatg tactccaccg ccgcctcggt
     1021 ttcgtggtaa ggcgcgtagt cgtcgtagat gtagggttcg ccctcgtttc tgaaaaaggt
     1081 ctgcttgaag tagcgatgcc gcatcctcgg ccgcaggctt tcgcgaaaac caagcgcctg
     1141 aggcgtgggc gccaggtgcc acttgccgat cagaaccgtg tcataaccgt tcgccgcaag
     1201 cacctccgcc agagtcggcg tctgattcgt cggccgaccc ggtcccgctt cattgtcttg
     1261 attgcgcagc acgcccgtgg aacgggcata tcggccggaa agaagaatcg atctcgccgg
     1321 cgaacaaacc ggaaaattgc tgaaagcaaa ctccattcgc acgcccgtgg ctgccagacg
     1381 atcgatactc ggggtccgca ccaccgcgtg accgtagcag ccgacctccg aggcgcgaag
     1441 ttgatcggga aaaacgatta ccacattggg ccgtctggat gaaggcgcga cccgctcatg
     1501 atccgccggc ccggccgccg ccggaatcac cggcgtcaca acccacaggc aaatcagtga
     1561 aatcgctcgc atgacaagaa atcctcccaa caggcaatcc gtatctcgct cgcgagtata
     1621 accatgccga tagcccggga aaaccggcaa tcgggctgcg tgacagtctc ggcgggcatg
     1681 ccgaccggcc caggttgggc gatcaacccc ctgataccct tgccgaggtt aatcacaggc
     1741 gcgcccagcg gccccgggga cgcatgcgga gcaccgcgtg cttgccaata tcccacgccg
     1801 cgacgatgat ctacgcaccg gggtacactc gatattcgtg attgctccca cccggcaccg
     1861 tgaaggagag aatatgaaaa ggtggttgac taccctcgtg attgtggccc agacggtgtg
     1921 cctcctgccg gccggccccg cccacgccga tgacgtgcgc aagtcggtcg tcaaggtctt
     1981 ctcgaccaag agccccccaa atatgttccg cccgtgggag atcaccccgc cccaggaggt
     2041 taccggctcc ggcgtcatca tcgaaggagg gcgagtcctg accagcgccc acgtcgtctc
     2101 gtggagccag caaatctatg tccagcccaa cgagtcttca gaaaagctcg atgccaccgt
     2161 cgagtacatc gccgaggatt gcgacctcgc catactcacc attgatgacg aagatgcaat
     2221 caaggacatc aagccggtcc cgctggccga aaagcttccg ccgccgaaaa ccaaagtgaa
     2281 cgtgctcggt taccccgtcg gcggtgatac gctctccgtt accgaaggcg tggtctcccg
     2341 catcgaatgg accaactaca actacggcac ggccgccttg cggatccaag tggacgcggc
     2401 catcaacccc ggcaacagcg gcggccccgc tatcgtccag gacgccgtag ccggcatcgt
     2461 cttcagccgc ttccccgaag gcgagaacat cggttacctg atcccggccg aggtcgtcag
     2521 gcatttcatc gacgacttca agaccgacgg aaaatacgac ggattcccaa aaatcgacgt
     2581 ccgcaccgcc acgctcgaaa atcccagcct gcgcagctac ctgaacatcg aacgtcagca
     2641 gaccggcgtc gtcgtgcacc gcgtggacca accggatctc aaggagaaga tcaaaccttg
     2701 ggacatcatc tccgcctgcg acggcgtcga aatcgacaac ctcggagtgg tgcctatcgc
     2761 tgaggacatc cgcgtcgaat gggggtatct catctccaaa aaggcgcccg gcagcacggt
     2821 gaagctgaag ctcatccgac aaggcaaacc agccgaggtc gaggtcgcga ccatcactaa
     2881 agacaatggc attatccaac ggatgacccg gacacggccg acctatttcc tgtacggcgg
     2941 cctggtgttc tcgccggcaa cggccgaact cgtctcggtg atccccccca gatacgttgc
     3001 catgctcggg tcgcgaggct ctctgctgtt caaacgagcc gatgaggacc gcgtcgagcc
     3061 gggcgacgag atcgtcgtcg cctgcagcgg catcctgccg cacagaatca ccaagggcta
     3121 cggcatcagc ccgctctcgg tcctcaccca cgtcaacgac cagcccgtaa aaagcctccg
     3181 ccacgccatc gagctgatca aggccggcaa ggataaggat tatgtcgtct tccgcttcga
     3241 ggaggaaaat gaggcaaaaa tcgtcctcga acccaagctg gttgagaaat tcacccccga
     3301 tatcttgcga aacaacaaca tcccaagcga ccgatcggag gatctgaaag atgtgtggcc
     3361 gtgatgccgt catgatccga agtctcgttg ctgttttgaa cttgtcatgg atcgggccgg
     3421 c
//
LOCUS       DAOTPW010000383         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_22886_length_3295_cov_5.675309, whole genome shotgun sequence.
ACCESSION   DAOTPW010000383 DAOTPW010000000
VERSION     DAOTPW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22886_length_3295_cov_5.675309"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="P5316_21185"
     CDS             <1..222
                     /locus_tag="P5316_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87528.1"
                     /translation="EHLRFPILISAQVMQASAFGKRPKKGRSGEETRCILCAEINRQL
                     KHQVLWFIAGRRQQAGQWAQALSAKTGTT"
     gene            complement(508..1956)
                     /locus_tag="P5316_21190"
     CDS             complement(508..1956)
                     /locus_tag="P5316_21190"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRR87529.1"
                     /translation="MATSIIWFRHDLRLADNPALLAAVEQGGSVIPVYIWSPEDEGDW
                     PPGGASQWWLHHALAALDADLRKRGSRLIIRSGRTVESLVALVRETKATAVFWNHRYE
                     PAAVQIAKDVENALRRQGLSVGEFHGNLLFDPRVTLNRQGGPFQVFTPYWKHCLAAPE
                     PELPKPAPGRISRPRRWPSSQSLNDLRLLPKVKWADGIAKDWTPGERAACHRLSRFIE
                     NALGDYSVGRDRPEKVGTSRLSPDLHWGHVSPRQVWHAVKRSTRRKSPKVATQAAGYL
                     RQLGWREFAHHLLVHFPHTPQEPLRPAFARFPWNGSPEHLRAWQHGRTGYPLVDAGMR
                     ELWHNGWMHNRVRMVVASFLVKHLLVPWQEGARWFWDTLVDADLANNTLGWQWCAGCG
                     ADAAPYFRIFNPVLQGRRFDPDGGYVRRWVPEIAGLGDKHVHEPWAAKAADLAECCIR
                     LGRDYPEPVIDHAEARKRALKAYDRLRGKPDR"
     gene            complement(2033..2548)
                     /gene="tpx"
                     /locus_tag="P5316_21195"
     CDS             complement(2033..2548)
                     /gene="tpx"
                     /locus_tag="P5316_21195"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRR87530.1"
                     /translation="MTERKGIVTFKGGPLTLVGNEVKVGDTAPEATLLANDLSPVRIG
                     SLRGKVLILSCVPSLDTPVCDAQTRKFNQAAAALGNDVEILTISMDLPFAQKRWCGAA
                     GIDRVRTLSDHRDAAFATAYGLLLKELRLLARAVLVVDKAGKVQYVELVKEVTQEPDY
                     EAALAAAKKLV"
BASE COUNT          634 a         1093 c          982 g          586 t
ORIGIN      
        1 gagcaccttc ggtttcccat attgatctct gcgcaggtaa tgcaagcatc agcgttcggc
       61 aaacgcccga aaaagggccg ttctggagaa gagacgcgtt gcattctttg cgcagaaatc
      121 aatagacaac tcaaacatca agttttgtgg ttcattgccg gtcgccggca acaagccggg
      181 caatgggctc aggcattgtc ggcaaaaacc ggtacgacgt aaaggccaga tcggatcggg
      241 gcctcaccga gcactctctt ctgtcgcgac tggtctgcat tttgcgcgca tgtttctgga
      301 tcgcagagcg cttgggttgc ggcccgtaaa aaggcggtcc gccttagctt gtatgacaaa
      361 tggctgatcc agaaccggtg gttgatcatc gaaacgccca agcgggccgc cgtcctttcg
      421 ggttttctct cacttgcggc gcgacaacac gttcacagaa tggctcatcc cgaaagagct
      481 gactcaacac gtgccgttga attgccttca gcgatcgggt ttgcctcgaa gacgatcata
      541 cgccttcaac gcccgcttgc gcgcttcggc gtggtcgatg accggctcgg gataatcgcg
      601 gccaaggcga atacagcatt cggcaaggtc ggctgccttg gcggcccagg gttcgtggac
      661 atgcttgtcc cccaagccgg caatctcggg aacccaacgc cgcacatagc cgccgtcggg
      721 gtcgaacctg cgaccctgca agaccggatt gaaaatgcgg aaatagggag cggcatcggc
      781 cccgcacccg gcacaccatt gccagccgag cgtattgttg gccaggtcgg catccaccag
      841 cgtatcccag aaccaccggg ccccctcctg ccacggcaca agcaagtgct tgaccaggaa
      901 cgacgcgacc accatccgca cgcgattgtg catccagccg ttgtgccaga gttcgcgcat
      961 accggcatca accagcgggt aaccggtgcg gccgtgttgc catgcccgaa ggtgctcagg
     1021 actgccgttc cacgggaagc gcgcgaacgc cgggcggagc ggctcctgcg gggtgtgcgg
     1081 aaagtgaacg agcagatggt gggcaaactc gcgccacccg agctggcgca gataacccgc
     1141 ggcctgcgtg gcgaccttcg gtgacttgcg acgggtagat cgcttcaccg cgtgccagac
     1201 ctggcgagga ctgacgtggc cccagtgcaa atcaggggag agccgcgaag taccgacctt
     1261 ctccgggcgg tcgcggccca ccgagtaatc acccaatgcg ttctcaataa agcggctgag
     1321 tcgatggcat gcagcccgtt ccccgggcgt ccagtctttc gcgataccgt ccgcccattt
     1381 gaccttcgga agcagccgca ggtcattcag actctgcgag gagggccatc gccgtggccg
     1441 cgagattctg cccggcgcgg gcttaggcaa ctccggctcc ggcgctgcga ggcagtgctt
     1501 ccagtacggc gtgaacacct ggaaaggccc gccctgtcgg ttgagtgtga cgcgcggatc
     1561 gaaaagcagg ttgccgtgaa actcgccgac ggatagacct tgcctccgga gggcattctc
     1621 cacgtccttg gcgatctgaa ccgccgccgg ctcgtagcga tgattccaga agacggccgt
     1681 cgccttcgtc tcacgcacca gggcgacgag cgattcaacc gtacgaccgc tccggatgat
     1741 caaccggctc ccgcgcttgc gcagatcggc atcaagggcg gccagcgcgt ggtgaagcca
     1801 ccattgactc gcgcctccag gcggccaatc gccctcgtct tcgggcgacc agatgtagac
     1861 cggaatcacc gaaccaccct gttcgacggc cgccagcaag gccgggttgt ccgccagccg
     1921 caggtcgtgc cggaaccaga tgatggaggt cgccatcgtg cacaaccgga acaggagtcg
     1981 tcgttagctc gcaacgatcg tggtgagcac atcctacacg gccgggccag tctcacacca
     2041 gcttcttggc cgccgccaag gccgcttcgt agtccggctc ctgtgtgact tccttgacca
     2101 gctcgacgta ttgcaccttg cccgccttgt cgaccaccag caccgcccgg gccagcaggc
     2161 gaagctcctt gagcagcaag ccataggccg tcgcaaaggc ggcatcacgg tggtccgaga
     2221 gcgtgcgaac gcgatcgatg cctgccgctc cgcaccaacg cttctgggca aacggcagat
     2281 ccatgctgat ggtaaggatc tccacatcgt tccccaatgc ggcggccgct tggttgaact
     2341 tccgcgtctg agcatcgcag accggcgtat ccagggatgg aacacacgac aggatcagca
     2401 cctttccgcg cagcgagccg atccggacgg gcgacagatc gttggccaac agcgtcgcct
     2461 cgggcgccgt gtcgcccact ttcacttcgt tgcctaccag tgtcagcggc ccgcctttga
     2521 aggtaacgat gccctttcgt tcggtcatgg tcctgggtct cctttcatgc gattgcaccg
     2581 tggacgcggt tttgctcggt gcgctgctgt cagggacagc ccaccggtcc acaggtcaac
     2641 cgattgacga gctattctat agccccctgg aagcgcgaga ccaccgcaag ccggttttct
     2701 ccccaaatcg agcccacggc tcccaccaag ccccgtcctc ggccgcgaag acccgcaggt
     2761 tggctgttcc gccagggcag atggggcttt gcccgatgat tcactgaacc gctgaacacg
     2821 ccgaccacgc ggaagaagga cgcgggaagt ctttccgatt attcagcgta ttccgcggtt
     2881 tatcttccct actcagcccc aagaggaccg ggagggccga cgagggcgtc ggccccacag
     2941 tagcgccgac gccctcgtcg gcgattcact cagccgcgaa accctgatca ggacgtgacc
     3001 cgtagcgccg acgcccccgt cggcgattca ctcagccgcg aaaccctgat cggcacgtga
     3061 cccgtagcgc cgacgccccc gtcggcgatt cactcagcag cgaaaccctg atcgggacgt
     3121 gacccgtagc gccgacgccc tcgtcggcga ttcactcagc cgcgaaaccc tgatcaggac
     3181 gtgacccgta gcgccgacgc ccccgtcggc gattctcaat cccacttcag gatcgcaccc
     3241 gtgctcgcgc tggtggccag ggcgctgtac ttggccaggc agcccttggt gtagc
//
LOCUS       DAOTPW010000384         3291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_22925_length_3291_cov_4.279048, whole genome shotgun sequence.
ACCESSION   DAOTPW010000384 DAOTPW010000000
VERSION     DAOTPW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22925_length_3291_cov_4.279048"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="P5316_21200"
     CDS             <1..377
                     /locus_tag="P5316_21200"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRR87531.1"
                     /translation="RFVAILIEHFAGAFPMWLAPVQVFVASVSEKSAAYAVKVRDRLV
                     EADLRVELDVSAEKIGPKKHYARKHKIPYTLVVGEREAAEGSVNVNDRSGKTIGNESV
                     EAFIARCVKEVNERTVKPAASN"
     gene            467..1021
                     /gene="infC"
                     /locus_tag="P5316_21205"
     CDS             467..1021
                     /gene="infC"
                     /locus_tag="P5316_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRR87532.1"
                     /translation="MQRCNEQIRLPSVRLIDENNEQVGVVPTAEALRRAREAGLDLVE
                     VAPTERPPVCRIMDFGKFKYSQNQKKKKQKHHQQKLKEIRLHPDVGEHDRDIKVSRAV
                     KFLEDGDKVQFTMVFHGRERFHKEIGFDIFKLIIEQVGEKGRIERPPRIEGQRMVMVF
                     SPGKPGAKPAAAVVPAKPAAETPT"
     gene            1114..2370
                     /locus_tag="P5316_21210"
     CDS             1114..2370
                     /locus_tag="P5316_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HRR87533.1"
                     /translation="MPDLIPPHGGLKEPVNRTVPADRVHEFVREVASWPALPVSDADL
                     SSVYRIGDGGLSPLTGPMVKAVYDQVLRDVNIDHNGHKYAWSIPISLPVTKDLASALK
                     VGRRVRLTNSAGETVGALLIEDIFPWDKLAYIEAVYQTKRIDHPGGQMVMNDPGEMLI
                     GGQIEMLPQPKHPEYGQFVLTPRETRTLFAEKGWERVVAFQTRNPLHRAHEYALVYGA
                     EVLTRQGFKTGVVLNPLIGETKSDDVDAPTRMRTYRALLENRQLGEGDKDEELWRSKG
                     YDINDVALLLGLDIKMFYAGPKEAIMHAIYRQNFGFTDIIIGRKHADAPYFDGKDIWD
                     PLAAQEIFSQLTGELRIRPVKVGFAAYYEELGRVALVDECGKKGWKPIPISGRDVREA
                     LSAGRLPDPRVMRESTARILIEAMRK"
     gene            2374..>3291
                     /locus_tag="P5316_21215"
     CDS             2374..>3291
                     /locus_tag="P5316_21215"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRR87534.1"
                     /translation="MSVPRELIDTLAAAGFPVIAGHVTPDLDSLGAMLALARSLPSSE
                     AAIVLPVPLESQRLLFLMELSNGVPIADPERVGRADVVVIVDTASTKRVNVAGHWEAI
                     ADKLIVNIDHHTTNPDFGSINWVEDNASSTCELVWRLIRAAGWKLDPVTAGLLYAGIY
                     SDTAGFSLPSTTADCLDAAADLVRSGADVDRIGCRIARSQERHEFDLIRKVYHNTHVV
                     AEGRIAYSTLTYEEIASCGCSPQDIDDQVSIPRSLSGIRMALLFSEGEKGVIRINLRG
                     EPGTPVLPLAEKLGGGGHAYSAGVRIRGAM"
BASE COUNT          663 a          939 c         1065 g          624 t
ORIGIN      
        1 agcggtttgt ggccattctc atcgagcact ttgccggagc gtttccgatg tggctggctc
       61 ccgtgcaggt gttcgtggcg tcggtcagcg agaagagcgc cgcgtatgcc gtgaaggttc
      121 gggatcggct tgtcgaggcc gatttgcggg tggaactgga cgtcagcgcc gaaaaaatcg
      181 gccccaagaa gcactatgcg aggaagcaca agatcccgta tactcttgtc gtgggagagc
      241 gggaggcggc ggaaggcagc gtgaacgtca acgaccgctc cggcaagacg atcggaaacg
      301 aatcggtcga ggcgttcatc gcccgttgcg tgaaggaagt caacgagcgg acggtcaagc
      361 cggccgcatc gaattgaccg gcatgggccg gtcgcgcgag ccttgtcgtg ctcgcgtcag
      421 gacaaagcga ggcttatcag aaagagaggg ttgaaccatc gccaaactgc agcggtgcaa
      481 cgaacagatt cgattgccgt cggtccgcct gatcgacgag aacaatgaac aggtcggagt
      541 tgtccctacc gcggaagctc ttcgtcgggc tcgcgaggcg ggcttggatc tcgtggaggt
      601 ggccccgacg gaaaggccgc ccgtctgccg gatcatggat ttcggcaagt tcaagtattc
      661 tcagaaccag aagaagaaaa agcagaagca tcaccagcag aagctcaagg agatccgcct
      721 gcatccggat gtcggggaac acgaccggga catcaaggtg agccgtgcgg tgaagttcct
      781 tgaggatggc gacaaggtcc agttcaccat ggtctttcac ggacgcgaac gattccacaa
      841 ggaaatcggg ttcgatatct tcaagctgat catcgagcag gtcggcgaga agggcagaat
      901 cgagcgtccg ccgcggatag aaggtcagcg catggtcatg gtattctccc ccggcaagcc
      961 gggagccaag cccgccgccg cggtcgttcc ggccaagccg gcggcggaaa cgccgacctg
     1021 agccctggtt catgggtgca acgggcggtt caaacaccga gccgatgccg gtgagcgcgc
     1081 ccgtcttttt cccgaaggag tgttcgtctt ttcatgccag atttgatccc gccccatggc
     1141 ggcctgaagg aaccggtgaa ccgcacggtc ccagccgatc gagttcatga gtttgttagg
     1201 gaagttgctt cgtggccggc cttgccggtc agcgacgccg atctgtcaag cgtttaccgg
     1261 atcggcgacg gagggctcag cccgctgacc ggcccgatgg tcaaggcggt ctacgatcaa
     1321 gttcttcggg acgtcaacat tgaccacaac gggcacaagt acgcgtggtc tatcccgatt
     1381 tcgctgccgg tcaccaagga cctcgcctcc gccctcaagg tcggccggcg ggtacggtta
     1441 acgaactccg ccggtgaaac ggtcggtgcg ctgttgatcg aggacatctt cccgtgggac
     1501 aagctcgcct acatcgaggc ggtctatcag acgaagcgga tcgaccaccc tggcggtcag
     1561 atggttatga acgatcccgg cgagatgttg atcggcggcc agatcgaaat gttgccgcag
     1621 cccaagcacc cggaatacgg tcaattcgtg ctgacgccgc gggaaacgcg gacattgttc
     1681 gccgagaagg gctgggaacg cgtggtggcc ttccagaccc gcaatccgct gcaccgggcc
     1741 catgagtacg ccctggtata cggggccgag gtgttgaccc gtcagggctt taagacaggg
     1801 gtggtcctga acccgctgat cggggaaacc aaatcagacg acgttgacgc cccgacccgg
     1861 atgcgaacgt accgggccct gctcgaaaac cgccagctcg gcgaaggtga caaggacgag
     1921 gaattgtggc ggtcgaaagg ctacgatatc aacgacgtgg ccctgcttct tggtttggac
     1981 atcaagatgt tctatgccgg tccgaaggag gcgatcatgc acgccatcta tcgccagaac
     2041 ttcggtttca ccgacattat cataggtcgc aagcacgccg acgccccata tttcgacggt
     2101 aaggacatct gggacccgtt ggctgcccag gagatcttct ctcaactgac cggcgagttg
     2161 cggattcggc cggtgaaggt cggcttcgcg gcctattatg aggaactggg gcgcgttgcc
     2221 ctggtcgatg agtgcggcaa gaagggctgg aaaccgattc cgatcagcgg acgtgatgtt
     2281 cgcgaggcgt tgtcggccgg ccgattgccc gatccgcggg tcatgcgcga atcaacggcc
     2341 cggattctga tcgaggccat gcggaagtga cctatgtccg tgccccggga actcattgac
     2401 accttggccg ccgccggctt cccggtgatt gcgggtcatg tcacgccgga cctcgattcc
     2461 ctgggcgcga tgctggccct ggctcgatcg ttgccgtcgt cggaggcggc gatcgttctt
     2521 cccgttcccc ttgaaagcca gaggctcctg tttctcatgg agctgagtaa tggcgtgccg
     2581 atcgctgatc cggaaagggt cggtcgcgcc gacgtcgtgg tgatcgtcga taccgcgtcc
     2641 acgaaacgag tcaacgttgc cggccactgg gaggccattg ccgacaagct gatcgtcaac
     2701 atcgaccacc acactaccaa tcccgatttc ggatccatca actgggtgga agacaatgcc
     2761 agcagcacgt gcgagctggt atggagactc atccgcgcgg caggctggaa actcgatcct
     2821 gtcactgcgg gactgctcta tgcggggatc tattccgaca cggcgggttt ctcgttgccc
     2881 agtacgacgg cggactgcct tgatgcggcc gcggatctgg tccgcagcgg agccgacgtt
     2941 gatcggatcg gttgccgaat tgcccggtcg caggagcggc acgagttcga cctgatacgg
     3001 aaggtctacc ataacactca tgtggtggcc gaaggccgca tcgcgtacag caccttgact
     3061 tacgaggaaa tcgcctcatg tgggtgctcg ccgcaggata tcgacgacca ggtctcgatc
     3121 cctcgctcgc tgtccggaat ccggatggcc ctgcttttct cagaaggaga gaagggtgtg
     3181 attcggatta atcttcgcgg cgaaccgggc acgccggttc tgccgctggc ggagaaactc
     3241 ggtggaggag gtcatgcgta ctcggccggg gttcgtattc gcggagcgat g
//
LOCUS       DAOTPW010000385         3265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_23152_length_3265_cov_4.828349, whole genome shotgun sequence.
ACCESSION   DAOTPW010000385 DAOTPW010000000
VERSION     DAOTPW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23152_length_3265_cov_4.828349"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..1711
                     /gene="nusA"
                     /locus_tag="P5316_21220"
     CDS             389..1711
                     /gene="nusA"
                     /locus_tag="P5316_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRR87535.1"
                     /translation="MNVELVRIVDSIARDKNIEKEVVFADLEQAMVSAVRKAHGPNDE
                     VVVNIDRLSGEITASCNNQPIDMKELGRIAAQTAKQVMIQKIREAERGSIYEEFLERK
                     GTVVTGTVSRFEGGALIVNLGRTEGFLPRSEQIPGETHHPGERIRCLILDVREAPHQV
                     KIVLSRSHPDFISRLFELEVPEVAERIIEIRALAREAGYRTKIAVSSIDSKVDAVGAC
                     VGVRGSRIKNIVLELGGEKIDIVRWNESSQILIGNALKPAEVQETALCFELGRATVVV
                     AEDQLSLAIGKRGQNVRLAARLTGWDIDILTPAEYNKNLDELEKVLSEIEGVGEDKIA
                     KIQALGIISLGDVEEVGAAPLVKDVGLSEELAAQVVSIASEAAKRLAIETAAAKALAV
                     EAAAEAAAAGEAAMAEDGTQASESTMVAEDGTSASESTGGNSEAPAEG"
     gene            1818..>3265
                     /locus_tag="P5316_21225"
     CDS             1818..>3265
                     /locus_tag="P5316_21225"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRR87536.1"
                     /translation="MSDKLRVHTLSKELGVTSKAIIEKCKSEGVEGVTNHMSALSAGL
                     AETIREWFSAAGHKTAVEEAAPVDIDAVRIKRKGTRRKKAEAPKETAVEEAGEQVVVA
                     AVVAEPAPPTAPAEVVVVPPVVETRAGGAEQAPPAVVIGEPEPPSSPPAEAPPAVPAE
                     EPVVAKPAAQAPAAAPPKPPEPVRPAGPQNVPAPAQMRGPKIVGYARPDPVTRPVPRA
                     PKGPAVVPEPLEFEGGGHKRGKGGKGPVYDDVPGKKGRQRLNPRRSTTSLYEIGERLR
                     EWNDRDLLERQERLQSASGRGIHHRAREKAAESAPARIAPRKTKAEVTEPIILHELCS
                     ATGVGMAQIMPKLMHDHNMGLVHRNTVIPTEIAEMVMLDFGVEIQVVKPKTELDKLIE
                     EFASRERKNPQPRPPVVTMLGHVDHGKTSLLDAIRKTSVAAGEAGGITQHIGAYRVER
                     GNLSVTFLDTPGHEAFTAMRARGAHLTDVVVLV"
BASE COUNT          636 a          891 c         1185 g          553 t
ORIGIN      
        1 gcccatgtac ctgaatcttg atcagataca tgtgtttgag ccgggcgaag aaggtgtcaa
       61 tgtgggtctg aacggtgccg gagaacaggt cagtgcggcc cgagcggttt gatagccgtg
      121 ggcaggaccc cgcagccggt cgccgctgca tcgcaaggag gacggcgatg cactaagcga
      181 tcgtcctgta ccttcgtttt gtgtatgccg tcttgtcccg agtgggatgg cggctcaaga
      241 agtgggtccg gcgaacgtcc cacttttttg tttgtcctgg ccggttcgag gcgagcgagt
      301 gcggggagaa cggcagcgca agcttccggc gaggcaagag aaagccagac agggcgaatg
      361 gaagcccttt cagcagaggt gatgcgagat gaatgttgag cttgttcgga ttgtcgacag
      421 catcgctcgg gacaagaaca tcgagaagga ggtcgtgttt gcagacctcg aacaggcgat
      481 ggtgtccgcg gtccgcaagg cccatggtcc gaacgatgag gtcgtggtga acatcgaccg
      541 gcttagcggc gagatcactg cgagctgcaa caaccagccg atcgacatga aggagctggg
      601 gcgtatcgcg gctcagaccg ccaaacaggt gatgatccag aagatccgtg aggcggagcg
      661 gggcagcatc tacgaggagt tcctggagcg caaggggacg gtggtgacgg gtacggtgtc
      721 gcggttcgag gggggcgccc tgatcgtcaa cctgggtcgg accgagggtt ttttgccgcg
      781 gagcgagcag attccgggcg agactcatca tccgggcgag cggatccggt gcctgattct
      841 ggacgtgcgc gaagcgccgc accaggtcaa gatcgtgctg agccggtcgc accccgattt
      901 catcagccgg ctgttcgagc tggaagtgcc cgaggtagcc gaacgaatca tcgagatccg
      961 ggctttggcg cgcgaagcgg gctatcgcac gaagatcgcg gtgtcgtcga ttgacagcaa
     1021 ggttgacgcg gtgggtgcgt gcgtgggagt tcggggcagc cggatcaaga acatcgttct
     1081 ggaactgggg ggcgagaaga tcgacattgt tcgctggaat gagtcgtctc agatcctgat
     1141 cgggaacgcc ctgaagccgg cggaggtaca ggagaccgcc ttgtgtttcg agcttggccg
     1201 ggcaaccgtg gtggtggcgg aggatcagct ttcgctggct atcggcaagc gcggtcagaa
     1261 cgtgaggctt gccgctcggc tgacgggttg ggacatcgat atccttacgc cggcggaata
     1321 caacaagaac cttgacgagt tggagaaggt gctgagcgag atcgagggag tgggggagga
     1381 caaaatcgcg aagatccagg cgttgggcat tatctcccta ggagatgttg aagaggttgg
     1441 cgctgccccg ctggtgaaag atgttgggtt gtcggaggag ctggccgccc aggtggtcag
     1501 cattgcctcg gaggcggcca agcggttggc catcgagacg gcggcagcca aagccctggc
     1561 cgttgaagcc gctgcggagg ctgcagcggc gggtgaagcg gctatggccg aggatggaac
     1621 gcaggcatcc gaatcgacta tggttgccga ggatggaacg tcggcatccg aatcgactgg
     1681 tggcaacagt gaggcaccag ccgaaggctg agaatcacgg gtccgggatg ggatctttcc
     1741 gccgttgagc cggccatcct gtgcaaattg gccggacggc ggggggggat ccatgaggcc
     1801 ggttcaggag gttgaatttg tcggataagc ttcgggtcca cacgctgagc aaggaactgg
     1861 gcgtcaccag caaggcgatc atcgagaagt gcaagagcga aggcgtcgaa ggggtgacga
     1921 accacatgtc cgcgctctcg gccggcctgg cggagacaat ccgggagtgg ttcagtgctg
     1981 ccggccacaa gacggctgtt gaggaagcgg cccccgtgga catcgatgcc gtgcgtatca
     2041 agcgcaaggg cacccggagg aagaaggcag aggctccgaa ggagacggcg gtcgaggaag
     2101 cgggtgagca ggttgtggtt gcggctgttg ttgctgagcc cgcccccccc acggcgccgg
     2161 cggaggttgt ggttgtcccg ccggtcgtgg aaacacgggc gggtggggcg gagcaagctc
     2221 caccggccgt cgtgatcggc gagccggagc ctccgtcatc tccgccagcg gaagcgccgc
     2281 cggcggtccc ggccgaggag cctgttgtgg ccaagccggc tgcccaagct cctgcggctg
     2341 ccccgccgaa acctcccgag cccgtgcgtc cggccgggcc gcagaatgtg cccgcccctg
     2401 cccaaatgcg cggacccaag attgtcggtt atgctcggcc tgatcctgtg acgcgccccg
     2461 ttccgcgggc acccaagggg ccggcggtgg ttccggagcc gttggagttt gaggggggcg
     2521 gacacaaacg cggcaaagga ggcaagggcc cggtctacga cgatgtgccg ggcaagaagg
     2581 gccgccagcg gctcaacccg cggcgctcaa ccacctctct ctatgagatt ggcgagcgtc
     2641 ttcgggaatg gaacgaccgt gacctgctcg aacgtcagga gcgcctgcag tcggccagcg
     2701 ggcggggcat ccatcatcgg gcaagggaga aggccgccga gtcggcgccc gctcgaatcg
     2761 ccccgcgcaa gaccaaggcc gaggtcaccg agccgatcat tcttcatgag ctgtgttccg
     2821 ccacgggcgt ggggatggcc cagatcatgc cgaagctgat gcacgatcac aacatggggc
     2881 tcgtgcatcg caacacggtg attcccaccg agatcgccga gatggtgatg ctggatttcg
     2941 gggtcgagat acaggtggtc aagccgaaga cggagctcga caagctgatt gaagagttcg
     3001 cgagccgcga gcgcaagaac ccgcagcctc gtcctccggt tgtaacgatg ctcggccacg
     3061 ttgaccatgg caagacgagc ctgcttgacg cgatccgaaa gacctcggta gcggcgggtg
     3121 aggcgggcgg catcacccag cacatcggcg cttaccgtgt ggagcgcggc aacctgtcgg
     3181 taacgttcct ggatacgccg ggccatgagg ccttcacggc gatgcgggcg cgaggggccc
     3241 atctcaccga cgtcgttgtt cttgt
//
LOCUS       DAOTPW010000386         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_23299_length_3249_cov_5.211647, whole genome shotgun sequence.
ACCESSION   DAOTPW010000386 DAOTPW010000000
VERSION     DAOTPW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23299_length_3249_cov_5.211647"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="P5316_21230"
     CDS             complement(<1..448)
                     /locus_tag="P5316_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87537.1"
                     /translation="MARQEGTILHTIKNDSSMPVETDSSPGGKPRGARRAVVRLLVSL
                     TVAYLGWCAVLFFWQDEMLFPRYSAPSPSSPPPSANVQIVRIDVGEKRHMEGWFFPAA
                     SASLSSPAPVVIYCHGSAEIIDQQEWFVENYHRLGCSVFLPEYRGYG"
     gene            632..1051
                     /locus_tag="P5316_21235"
     CDS             632..1051
                     /locus_tag="P5316_21235"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRR87538.1"
                     /translation="MATAVELRAVDLIRTEIERFADAARRRSECDPVHRRRSRRRYHR
                     SWPLAVLYDGVEMSAALHNASDEGIAFLSCGPIEPGRIVFIKLFCYEESCPYVPAVVR
                     HSTPTEHGHLIGCEFELADERVCQEAIKYSHQEVGLC"
     gene            complement(1108..1404)
                     /locus_tag="P5316_21240"
     CDS             complement(1108..1404)
                     /locus_tag="P5316_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87539.1"
                     /translation="MSDAQATALLEKLHSLQGVLSQVYGMINMVRPVASDVCFQCMQS
                     ATLANTSAIPGRTLAQVGEEFADAVESVANSMATASGSLAILIGALSAGNAEMA"
     gene            complement(1464..2618)
                     /locus_tag="P5316_21245"
     CDS             complement(1464..2618)
                     /locus_tag="P5316_21245"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRR87540.1"
                     /translation="MNASIGFQISPSEQGLKPYAVTSVNTRGRDHPERFGRNMDPYAI
                     DRQRVLHSVAFRRLAHKTQVFVTGEHDHLRTRLTHTLEVTSVVRRLCVALQVNGAVGE
                     LIAMTHDLGHPPFGHAGEVALHRLMAHHGGFEHNAQSLRIVEYLEHPYPAFRGLNLTY
                     EVRESLAKHCTIYDRPGLHRLADGSQAPLEGQIANLADRVAYDCHDLEDAMGEGLLDE
                     AVLKEVDLWSEAAAPIRAEHSDLPLAAVRRPILDRLEAILLEDIVAESRRRIASSAVA
                     CVDDVRNCRQPLIAFSRSMEPRVSGLEAFLSERVYRHSRLIRMDDKAGRLIDQLFNVY
                     LAEPKLLPPRFERRVKEQGLHRVICDYIAGMTDRFCQEEFRRLFEPFERV"
     gene            complement(2672..3241)
                     /locus_tag="P5316_21250"
     CDS             complement(2672..3241)
                     /locus_tag="P5316_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRR87541.1"
                     /translation="MPLRMPPVKLQLVMAEFSRNLWAPWRMEYIRSLGGDRPDNGCFL
                     CRYAATPQADGENHVIWRGSCCLTAFNSFPYNSGHLLVAPLGHVADLDLMDDVSLSEL
                     MCQIRDAKRLLTAVLAPQGFNIGMNFGKCAGAGLPGHLHVHIVPRWEGDTNFMPVVGD
                     TRVIPESLAAIHDRMKAAAADLGLPGIRG"
BASE COUNT          673 a         1013 c          972 g          591 t
ORIGIN      
        1 gcccatagcc tcgatattcc ggcaggaaaa cggagcaacc caaccgatgg tagttttcca
       61 cgaaccactc ctgctggtcg atgatctcgg cgctaccgtg acagtagatc accacggggg
      121 ccggcgagga cagactcgcc gaagccgcag ggaagaacca accctccatg tgccgctttt
      181 cgccgacgtc gatccgcaca atctggacgt ttgcagaagg cggcggcgac gacggcgagg
      241 gagcgctgta gcgtggaaag agcatctcat cctgccagaa gaaaagcacg gcgcaccagc
      301 cgaggtatgc gaccgtcagc gacacaagga ggcgcacgac cgcccgtctt gctcctcgtg
      361 gttttccgcc gggggaagag tccgtctcga ccggcatgga cgaatcattc ttgatcgtgt
      421 gcagaatcgt gccctcctgt cgcgccacgg gaatcgtcga gcggccggat ggagtgtatc
      481 ggactcaaga ggtaatgaca atctcgtctc gcgcgggcgg ctaagcccct attgcggccc
      541 ctcgggccgc tcccggccgc tcttaccgga cgtctgttcc cagtttggca tcggtcccgc
      601 tcgaatcgta tatccatatc cggggacaag catggccacc gcagtagaac tccgtgccgt
      661 cgatctgatc cgaactgaaa tcgagcgatt cgcggatgcc gcccgccgca ggagcgaatg
      721 cgatcccgtg catcgccggc gatccaggcg tcgataccac cgctcctggc cgctggccgt
      781 gctttacgac ggggtggaga tgagtgccgc cctgcataat gcctccgacg aaggaatcgc
      841 ttttctgtcc tgcggaccga tcgagccggg tcgcatcgta ttcatcaaac tgttctgcta
      901 tgaggaaagt tgcccgtatg tgccggccgt cgtccgccat tcgacgccaa cggaacacgg
      961 ccacctcatc gggtgcgagt tcgaactggc cgacgaaagg gtctgtcagg aggcgatcaa
     1021 gtactcgcat caggaagtgg gattatgctg atgtgccgtt cacgggttgc ggcacgaagg
     1081 tccggactgt gacgtccccc ggagcaatca cgccatttca gcattaccgg cactgagcgc
     1141 cccgatcagg atggccagac tgcccgaagc cgtcgccatg gaattggcaa ccgattcaac
     1201 ggcatccgcg aattcttccc ccacctgagc aagagtccgg ccggggatgg cgcttgtgtt
     1261 cgccagcgtc gccgactgca tacactgaaa gcagacatct gaagccaccg ggcgaaccat
     1321 attgatcatg ccgtacacct gactcaacac cccttgaagg gaatgaagct tctccagcag
     1381 tgcggtcgcc tgagcatcac tcacgtcgcg gtctcctatc gcatggacaa tccgacgaca
     1441 ccctggccgc cgggttcggt atcttacact ctctcgaaag gttcaaacaa cctacggaac
     1501 tcctcctggc agaagcgatc ggtcatgccc gcaatgtagt cgcagatgac ccggtgcagc
     1561 ccttgttcct tgactcggcg ttcgaaccgc ggaggaagaa gcttgggctc cgccaggtat
     1621 acgttgaaaa gctgatcgat cagacggccg gccttgtcgt ccatacgaat cagccgacta
     1681 tgccggtaga ctcgttcgga cagaaaggct tccagaccgg aaacgcgcgg ctccatgctc
     1741 ctggaaaagg caatcagcgg ctgtcggcag ttccgcacat catcgacgca cgcgaccgcg
     1801 cttgaggcga tccgccgccg tgattccgcg acgatatctt cgagcaagat cgcctccaac
     1861 cggtcgagaa tcggacgtct gaccgccgcc aggggcaaat cggagtgctc ggcccggatc
     1921 ggagccgcgg cctcagacca caagtcgacc tctttcaaaa cggcctcgtc caggagcccc
     1981 tcgcccatcg catcttccag gtcgtgacaa tcgtatgcca cgcggtcggc cagattggcg
     2041 atctgcccct ccagcggagc ttgagaaccg tcggccaacc gatgcagacc cggccgatca
     2101 tagatcgtgc agtgcttggc cagggactcg cgcacttcat aggtcaggtt cagtccccga
     2161 aaggccggat aaggatgctc gagatactcg acaatccgaa gcgactgcgc gttgtgctcg
     2221 aaaccgccgt gatgggccat cagtcggtgg agggcgacct ccccggcgtg gccgaagggt
     2281 ggatgcccga ggtcgtgagt catggcgatc agttcgccga ccgccccgtt gacttgcagt
     2341 gccacgcaca accggcggac aacgctggtc acctccaacg tgtgggtcaa ccgggtccgc
     2401 agatggtcat gctcgccggt gacgaagacc tgagtcttgt gagccagacg ccggaaggca
     2461 accgagtgca acactcgctg ccggtcaatg gcatagggat ccatgttccg accgaaccgt
     2521 tccggatgat cccggccgcg ggtgttgacc gacgtaaccg catagggctt gagcccttgt
     2581 tcgctcgggc tgatctggaa accgatactg gcgttcataa ccggcgcctc gcaagaccgc
     2641 tggatgcagg tgattcaggg gcggaattgg ctcatccgcg aattcccggc agacccaggt
     2701 cggcggccgc ggctttcatt cgatcgtgga tggccgcaag gctttcgggg atcactctgg
     2761 tgtcgcccac gacgggcatg aagttcgtgt ctccctccca acgaggaacg atgtgaacgt
     2821 gaagatggcc aggcaagccc gcaccggcgc actttccgaa attcattccg atgttgaacc
     2881 cctgcggggc aagtacggcc gtcaggaggc gcttggcatc acggatctgg cacatcagct
     2941 cgctcagcga aacgtcgtcc atcaagtcca gatcggccac atgcccgagg ggagccacga
     3001 gcagatgtcc ggagttgtac ggaaagctgt tgaatgccgt caggcagcat gagccgcgcc
     3061 agattacgtg gttctcgccg tcggcttggg gggtagcggc ataccggcaa agaaaacagc
     3121 cattatccgg ccgatcgccg ccgagcgaac ggatatactc catgcgccag ggagcccaca
     3181 aattgcgact gaactctgcc ataacgagct gaagtttaac tggcggcatc cgcaggggca
     3241 cgcctttgc
//
LOCUS       DAOTPW010000387         3218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_23577_length_3218_cov_4.978185, whole genome shotgun sequence.
ACCESSION   DAOTPW010000387 DAOTPW010000000
VERSION     DAOTPW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23577_length_3218_cov_4.978185"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="P5316_21255"
     CDS             complement(<1..1002)
                     /locus_tag="P5316_21255"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87542.1"
                     /translation="MSRYLDSIIVAGYFIAVFVAGALLARRFARRETSEFLTGGRTFS
                     WHQTALTLLAFAVDPTYMGMAGIAFIWGMYVSQWTGVHIWLTSWVAAMFLVPIYWRTR
                     IVTTPEYLERRFNAQCRALFSVLMAVILVIILASAIYLGALLTTRLLDWPLSLSVALI
                     AVVCTFYVIVGGLRTVLSLDVYQGAILLVTLIVVAWRVVTEVGGLGALAAIDVPGSAG
                     TPIGSMVPPLEFDLRSETFFPVPAVLAWATVAGLSWIACNFGMVQRLLASRTEQDAQK
                     SLLLLAVLANIACFLTFAVGTAMRKLHPGLPADQAFMEVMLTMFPTGVRGLLVAGMMA
                     "
     gene            complement(1040..2086)
                     /locus_tag="P5316_21260"
     CDS             complement(1040..2086)
                     /locus_tag="P5316_21260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR87543.1"
                     /translation="MPIPRLAANLAGGDTANDPGWGDWEIRITSLQRGRMSKMTIRPF
                     TGMDLKEVLAVWNRALKKDPMTEGRFVRWALADPDYWPGDDSGFFVALQDDRLVGFLR
                     AIIRRMPNDRAGLEPELGWIPVVAVDPPCRRHGIGFALLESALAWFRKHGRRRIWVCG
                     NSGSAPGYVFPGVDKDDYPGGLALFAKAGFRVHRGSVAMSREVVNFDIDEYRRQAWET
                     GKDVRISTLHPEQVPDFFTFLSEAFPGDWNTAARAKIRAGLLHEVLVATADGRIVGYC
                     QWEGEHFGPFGVRAEYRNRRIGAKLFVEAVQKIREADGRTVWFNWAEDDAARFYGRFG
                     LTATRRYCIFVKEL"
     gene            2434..>3218
                     /locus_tag="P5316_21265"
     CDS             2434..>3218
                     /locus_tag="P5316_21265"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRR87544.1"
                     /translation="MKWVFLGILGVLIAWSLLAYAMMPDASRDGRTVLTWVSDDNPAR
                     RGSIRLFEQLNPDLRLLLDPANTTLEKIIVQCKGGVGGDVFNCYGRWQLVDFVRAGVL
                     QPIDEEAARLGFDVAKTWPEVLDEISIEAFNPAAGRYERHQYTFPLNVNANVMFFNKR
                     IFDEVGEPYPTSDWDWNECLRVAKKLVKRDRHGRITRYGLGTYEMHETVGLVWQFGGS
                     FFDPTLTYCTLDSSEAIEAMRFLHRMLVVDEVMPSVAERNAIGT"
BASE COUNT          630 a          942 c         1064 g          582 t
ORIGIN      
        1 ggccatcatg cccgccacga gcaggcctcg gacgccggtg gggaacatgg tgagcatgac
       61 ttccatgaaa gcctgatcgg ccggcaagcc cggatgcaac ttgcgcatgg ctgtcccgac
      121 ggcgaatgtc aggaaacagg cgatattggc gagtacggcg agaaggagca ggctcttctg
      181 ggcgtcctgt tcggtccgcg aggcaagcag gcgctggacc ataccgaaat tgcaggcaat
      241 ccaggacaga cctgcgaccg ttgcccaagc caggaccgcc ggtactggaa aaaacgtctc
      301 ggatcgcagg tcgaactcca gcggcggcac catgcttccg atgggcgtgc cggcactgcc
      361 cggtacgtcg atggcggcca aggctccgag gccgccgacc tccgtcacca cgcgccaggc
      421 gacgacgatc agcgtcacca gcaggatcgc accctggtac acgtccagcg agagcaccgt
      481 gcgcagcccg ccgacgatca cgtagaaggt gcacacgacg gcgatgaggg cgacgcttaa
      541 tgaaagaggc cagtccaaca accttgtcgt cagcagcgcg ccgagataga tcgcgcttgc
      601 caggatgatc accagaatta ccgccatcag caccgagaac aatgcccggc actgtgcgtt
      661 gaatcggcgt tccaggtatt cgggcgtggt gacgatccgc gtgcgccagt agatagggac
      721 gaggaacatg gcggcgaccc agctcgtcag ccagatgtgg acgccggtcc attgcgacac
      781 gtacattccc cagatgaagg cgatgccggc catgcccatg tacgtcgggt ccacggcgaa
      841 tgccaggagc gtcagagcgg tctgatgcca cgagaacgtc cggccgccgg tgaggaattc
      901 ggatgtctcg cgtcgggcaa agcgccgggc gagcagcgcg ccggccacga aaacggcgat
      961 gaagtaaccg gcaacgataa tggagtcgag atacctgctc atggctgaca ttgagtccgg
     1021 cgtggctgcg cgaacgcagt cacaactcct tcacaaagat gcagtagcgt cgcgtcgcgg
     1081 tcaggccgaa gcgtccgtag aagcgtgcgg catcgtcctc ggcccagttg aaccataccg
     1141 tccgcccgtc ggcctcgcgg atcttctgaa cggcttcgac aaaaagcttg gctccgatac
     1201 ggcggtttcg gtactccgcc cggacgccga acggcccgaa atgctcgcct tcccactggc
     1261 agtaaccgac gatccgtccg tcggcggtgg cgaccagaac ttcgtggaga agcccggcac
     1321 ggatcttggc ccgtgcggcg gtgttccaat caccagggaa agcctcagaa aggaaggtaa
     1381 agaagtccgg aacctgctca gggtgcaggg tactgatccg gacgtccttg ccggtttccc
     1441 aagcctgccg acggtactca tcgatgtcga aattgaccac ttcgcgggac atggctacgg
     1501 agccgcggtg gacgcggaag ccggctttgg cgaacaacgc caacccaccc ggataatcgt
     1561 ccttgtcaac gccggggaag acatatccgg gtgctgaacc gctgttgccg caaacccaaa
     1621 ttcgccggcg gccgtgtttg cggaaccaag ccaaggctga ttccagcagt gcaaagccga
     1681 tgccatggcg ccggcacggg ggatcgaccg cgacgacggg gatccaaccc agctcgggtt
     1741 caaggcctgc ccgatcgtta ggcattcggc ggatgatggc ccgcagaaag ccgaccaagc
     1801 ggtcgtcctg caaggccaca aagaagccgg agtcatctcc cggccagtaa tcgggatcgg
     1861 ccagggccca ccggacgaat cgtccttcgg tcatgggatc ttttttaaga gcccggttcc
     1921 agacggccag cacctctttc aggtccatac ccgtgaacgg ccttatcgtc atcttgctca
     1981 tgcggcctcg ctgcaatgat gtgatccgga tctcccaatc cccccagccg gggtcgttgg
     2041 cagtatcacc gccggccaga ttggccgcaa gtctggggat gggcatgtcc ggtttgggat
     2101 tcccgttctc cgttggtcgt gatcgaggga gcgggtagac tggagtgaac gcggggccga
     2161 tgcgaacggc ttgattggcc ggaggatggg attcgtgttc agcggcgtgg ctcatgcctg
     2221 gcagcgggct ctgtctcgtg atccaggggt tgggctgcga ttgccgcggc ctgtgagaac
     2281 gggtgtgaga ctggcagggc acggtcggag ccgccgcaag cggcgcagac ctgccgctcc
     2341 aggttcggac aatgcgggag gtctcggtcg ctgtggcctc gacgctgtcc tgccgtatgc
     2401 tccggtggtt ttcgcacatg ttctgggaac gatatgaagt gggtcttttt gggcattctc
     2461 ggtgtcctga tcgcctggtc gctgctggcc tacgcgatga tgcccgacgc atcgcgggac
     2521 ggccggacgg ttctgacgtg ggtcagtgat gacaacccgg cccgccgcgg cagtattcgt
     2581 cttttcgagc agctcaatcc agacttgcgg cttctcctgg acccggccaa caccacgttg
     2641 gagaagatca tcgttcagtg caagggaggc gtggggggcg acgtgttcaa ctgctacggc
     2701 cgctggcaac tggtcgattt tgtgcgggcc ggcgttttgc agccgatcga cgaggaggcc
     2761 gcacggctgg ggtttgatgt tgcgaagacc tggcccgagg tgctggatga gatttcaatc
     2821 gaggcattca acccggccgc cggccgatat gagcgtcatc aatacacctt cccgctcaac
     2881 gtgaacgcca acgtgatgtt cttcaacaag cggatctttg acgaggtcgg cgagccgtac
     2941 ccgacgagtg attgggactg gaacgagtgt ttgcgagtgg ccaagaagct ggtgaaacgc
     3001 gaccggcacg ggcggatcac gcgttacggg ctggggacgt atgagatgca tgagacggtg
     3061 gggttggtct ggcagtttgg cggatcgttt ttcgacccga cgctcacgta ttgcacgttg
     3121 gattcgtcgg aggccatcga ggcgatgagg tttctgcatc ggatgctggt ggttgacgag
     3181 gtgatgccgt cggtggccga gcggaacgcg atcggcac
//
LOCUS       DAOTPW010000388         3185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_23893_length_3185_cov_4.094888, whole genome shotgun sequence.
ACCESSION   DAOTPW010000388 DAOTPW010000000
VERSION     DAOTPW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23893_length_3185_cov_4.094888"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="P5316_21270"
     CDS             <1..530
                     /locus_tag="P5316_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87545.1"
                     /translation="DTDMPNEDNKPHLCVPTLDGNAHVIPTEVFEKIVSGEMKITDME
                     DWEIITRTAFSEWLRGLETAVKARVYKSAVDQLADSMAAGAWQDGPAPKDGSWILGLF
                     HGLPYVVGYDSWDVGGEMLPDGTGSPPDGHESGWCLAGDNFHVMDKDEPEKWARIIHP
                     NRHMPASWDGPGDLG"
     gene            complement(755..1240)
                     /locus_tag="P5316_21275"
     CDS             complement(755..1240)
                     /locus_tag="P5316_21275"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRR87546.1"
                     /translation="MNTITAFLIVCQAATSTNDPFPATLRAILWVESRGDPQAVGDGG
                     RAIGPYQIHRAYWEDATRFLGVAWPYEDARDPVKAARAVRAYTEHYANAYQRPWTAET
                     IARIHNGGPTGWKKAATMGYWGKVRAAMERQGRPQRTQRMARMKGSQPCSVRRWQSKC
                     R"
     gene            complement(1405..1638)
                     /locus_tag="P5316_21280"
     CDS             complement(1405..1638)
                     /locus_tag="P5316_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87547.1"
                     /translation="MDHEERWHEICQPKLKSIEEKLDMILTQLQGNGMPGYGDRIRDL
                     ERSRAAAAKLLWALVLAAIAQAAIWIRGLLIGR"
     gene            complement(2030..>3185)
                     /locus_tag="P5316_21285"
     CDS             complement(2030..>3185)
                     /locus_tag="P5316_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87548.1"
                     /translation="KVAATFTGDLFAPTAYSEFGDLRIEGQSQSHVFDAIVGATGDNP
                     NCRIYRMRFKYVGGVCIQMDASWGTDIVDVHCLDVGYGLRLEKFNGGTIRQFYVNGHH
                     DHAVVLSDSRACTIHGLILENSDDPGAVHRLYLSGVEGATIDGFYTEGTLGTGGRDIY
                     MSQKSGIGSCEGVLIRNAWMNSNSITGVAPIYAAYVRNCRFEDIHWHTPSNTYWGSPD
                     YYIENETGAAAEQTIYCRGWSNEDGSEDIALAKHNDFIMYDVTCGLTGDAETAFLTVA
                     DAAGHYPHLVGNNRQLAAVSHDYAAGTTAWNLTSIEAFASLLTVTNASGAADAVFAKA
                     IPGKMFWVYNNSGQAITFKVSGQAGVAVANGKRAILGMDATDVRELYEQP"
BASE COUNT          621 a         1002 c          896 g          666 t
ORIGIN      
        1 gagatacgga tatgccaaac gaagacaata agcctcatct gtgcgtgccg acgttagacg
       61 gaaatgccca cgtaattcca acggaagtgt tcgaaaagat cgtttccggc gagatgaaga
      121 tcaccgacat ggaagattgg gaaatcatca ccaggacagc cttttcggaa tggctgcgag
      181 gattggaaac ggcagtaaaa gcacgagttt acaaatctgc cgttgaccaa cttgccgact
      241 cgatggctgc cggagcatgg caagacgggc cggctccgaa ggacggttct tggattcttg
      301 gcctattcca cggcttacct tatgtggttg ggtacgactc gtgggatgtt ggcggggaga
      361 tgctgccaga cgggaccgga tcacctcctg atggccatga aagcggctgg tgtttggccg
      421 gagacaattt ccatgtcatg gacaaagacg agcccgaaaa atgggcccgg atcattcatc
      481 caaaccggca catgccggcg tcgtgggacg ggcccggaga cctcggttag gaaatgtaca
      541 tttgggaagg gaaatcccat gcaactcgcc acaattcgcc gcatcgcctg gacgctgccc
      601 ttttggatgg gaggccttgc cttccttttg gatctggcgt ttacgcaacc caccggccgg
      661 gaccgctccc cgcaactcct ccgcatggaa tccatggaaa ctctgaccgg ccaggacttc
      721 gaaagtgcgt ttgccacggg tcttctctgg gccgctatcg gcacttggat tgccatcggc
      781 gtacactaca cggctggcta cctttcatcc tcgccatcct ctgcgtcctc tgcggtctcc
      841 cctgccgctc catcgccgcc cgaaccttgc cccaataccc catcgttgcc gccttcttcc
      901 acccggtagg ccctccattg tggatccggg cgatggtttc cgccgtccag ggcctctgat
      961 acgcgttcgc gtagtgctcc gtataggccc ggaccgcccg ggcggccttg accgggtctc
     1021 tggcgtcctc gtacggccag gcgacgccca ggaagcgtgt agcgtcctcc cagtaggccc
     1081 gatggatctg atagggtccg atcgcccgcc cgccgtcgcc gacggcctgg ggatcgccgc
     1141 gggattcgac ccagagaatc gccctcagcg tcgcgggaaa agggtcgttg gtggaggtgg
     1201 cggcttgaca gacgatcaga aatgcggtaa tcgtattcat gctggaacct cctgaacagg
     1261 ttctggaagt cccggcgtcg gtgctaccaa caccacgccg gggcgttttt caaagcctac
     1321 caaagacatc ggccgccggc aagcggaagt tttgcctttt tcccatctcc gaaaaaaaag
     1381 tcgcggcaag gatctcctgt attctcatct cccgatcaac agtccgcgaa tccagatcgc
     1441 agcctgggcg atcgccgcca gcaccaacgc ccacaagagt ttcgccgccg cggcgcgaga
     1501 ccgttccagg tcccggatcc gatccccata cccaggcatg ccgttgccct gcagctgggt
     1561 caggatcatg tccagttttt cttcgattga cttgagtttg ggctggcaga tctcatgcca
     1621 gcgctcttcg tgatccatcc ctcgcctttc ccggtgggtg gtgtgtcatg gtgtcatggt
     1681 gattcaaagc tggtcgtgcc gatcgactgc gtcatcatcc agacgcccga ggccatacgc
     1741 tccagccaaa cttctgtgcc gacagccccc aatatcaagt acttgccggc agatccgccc
     1801 tggatcgttt cggatccgct cggatcaatc cggacttcat aaggggcgtt tttcaaagct
     1861 tacgattccc atcggctttt tccaaccgcg acttgagaag attcgcagag ggacctcatt
     1921 gaccttgttg acctcattga cagccgcggc acagccgtca atacgtcaat ggggtcaatc
     1981 aggtccggcc cgcatctgca ctctgcggag ggcgacgcat gcgtcgccct tatggctgct
     2041 cgtagagctc ccggacatct gtggcatcca tccccaaaat cgcccgtttg ccgttcgcga
     2101 ccgcgacgcc cgcctgtccc gagaccttga acgtgatcgc ctggccgctg ttgttgtaga
     2161 cccagaacat tttgcccgga atcgccttcg cgaagacagc atcggccgcg ccgctcgcat
     2221 tcgtcaccgt cagcaatgag gcgaacgcct cgatgctcgt gaggttccat gccgtcgtgc
     2281 cagccgcata gtcgtggctg accgccgcca gttggcgatt gttccccacg aggtgcgggt
     2341 agtgacccgc tgcgtcggcc acggtgagga acgccgtctc ggcgtcgccg gtcaggccgc
     2401 acgtcacatc gtacatgatg aagtcgttgt gcttggccag ggcgatgtcc tcgctgccgt
     2461 cctcgttgga ccatccgcgg cagtagatcg tctgctcggc cgccgccccg gtctcatttt
     2521 cgatgtagta gtccggggag ccccagtacg tgttcgacgg cgtgtgccag tggatgtcct
     2581 caaaacggca gttccggaca taggccgcgt agatcggcgc cacgccggtg atcgagttgc
     2641 tgttcatcca ggcgttgcgg atcaggacac cctcgcacga gccgattccc gacttctggg
     2701 acatataaat gtcccgcccg ccagttccaa gcgtgccttc ggtatagaag ccgtcgatcg
     2761 tcgccccttc tacgccgctg aggtacaggc gatgcacagc cccaggatcg tcactgttct
     2821 ccaagatcag gccatggatc gtacaggccc gcgagtcgga caggacgacc gcatgatcgt
     2881 ggtggccgtt gacgtagaac tgcctaatgg tgccaccatt gaatttctcc aaccgcaaac
     2941 catagcccac atcgaggcag tggacatcaa caatgtctgt gccccacgac gcatccattt
     3001 ggatgcacac cccgccgaca tatttgaatc gcatccgata gatccgacaa ttcggattat
     3061 cgccggtggc accgactatc gcatcgaaca catgcgactg ggactggccc tcgatccgca
     3121 aatcgccgaa ctccgagtac gccgtcggag cgaacagatc cccagtgaat gtcgcagcga
     3181 ccttg
//
LOCUS       DAOTPW010000389         3150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_24185_length_3150_cov_5.926010, whole genome shotgun sequence.
ACCESSION   DAOTPW010000389 DAOTPW010000000
VERSION     DAOTPW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24185_length_3150_cov_5.926010"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..252
                     /locus_tag="P5316_21290"
                     /pseudo
     CDS             55..252
                     /locus_tag="P5316_21290"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            433..711
                     /locus_tag="P5316_21295"
     CDS             433..711
                     /locus_tag="P5316_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRR87549.1"
                     /translation="MAKPDAIGFIETQGLVGMMEAADVMLKAANVEIIGKEKIGAAYV
                     TVMVRGDVAAVKTAVEAGAAAVNSLGGKLILAHVIPRPHEGLTRLLPA"
     gene            748..>3150
                     /locus_tag="P5316_21300"
     CDS             748..>3150
                     /locus_tag="P5316_21300"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR87550.1"
                     /translation="MTRNTLPQPATRPAGIRHGIVVFLAAFMAMLPALRGGFIEGDDE
                     QLVLNFVLVSRPSLSHALELLTLEPHRYLYQPVPMLSFAAEFALLKAVGLSALPDAPN
                     SGTWLFHLTNVLIHAAAALLVWRLIRRLHPDPLVALVAGILFAVHPMGSEPTAWVCGR
                     MISLSALFSLLSLNLLDAWEERPRIGLALLVLAAVVLAMTSKVQVGLPVLMLLLPLAR
                     RRWPRRTWWVIWGLSLLITGSFAVLNVTLTKQTHLLEVAAERLEGPRSVRVLLALGWY
                     LSRVVWPAGLAPFHPARWVVAWSDPDVLTAVLTLLVALVLVTISVRWTRIGVLGLIWF
                     LANVAATLPLLPSRSSMFAERYVYISNIGLFWAIAAFLVFGYRRWAEARPSVRWFSRV
                     AAALAVLMLVITTWQTVTAFRDDIRKNLRVAEVYPNIPGSWTRLGLAYYGAGQYEQAI
                     EAANEDMARHPAETSASVHRVIGMAYHKMKRIDDAIEALQKSLEADPHSYRTHRHLAE
                     VYREAGRTDEAMAHYRHALALAPMYEPALLGLGQMLQTAGQADEAVPLYERVLKDNPF
                     HLTANLAMAEIEIGRSQYNLAAARLQKLLSYAPESWTAWTNLGVCQVNLGQPGRALES
                     YRTALSINPQAEAAALNLAGLLISRGQGPEAARILVRALADTSDRQTLWTAYDLLVEG
                     GQLAAGADLWAGSLRREPDAADLKCSLAWALLLRQQSNESRKLLNECAGRLENDPVFD
                     AALAMLALGDGDPSPAVKWVESAARAEEGPRREMLPRMKQAVRLYGERHSDQPWPYYL
                     AAR"
BASE COUNT          550 a          999 c          961 g          640 t
ORIGIN      
        1 ttcacctggg ccacatgaac atcgactcgt tacagggggt gtccggtgaa cgctatgatc
       61 ctacccaacc tggagagcgt cggttttgtt gaggtgatgg gcctcggcac atccctggtc
      121 gtcgccgacg tcatggccaa ggccgcgaac gtgaccatag ccggaattga gtccaacgcc
      181 ctcggcggca tgggtgtcaa agtcaccggc agcaccggtg atgtgcgggc ctccgtcgag
      241 gccggttggg cgattgccga gcagatgcac gcggggatca accgaaccgt ctggccgaac
      301 tactcggccc aggccgactt cctcgttcac tcgaagcagg agttcaacgc tctgcttgaa
      361 gccaatgatc atcttctgcc gccggatgcc aacctgccgg ccggcagtac catgaaagga
      421 acggacaaca ccatggcgaa accggatgcg atcgggttta tcgagacgca gggcctcgtc
      481 ggcatgatgg aggcggcgga cgtgatgctc aaggccgcaa acgtcgagat catcggcaag
      541 gagaagatcg gcgccgctta cgtcaccgtg atggtcagag gcgacgtcgc cgccgtgaaa
      601 accgctgtcg aggcgggcgc cgccgcggtc aacagcctcg ggggcaaact catcctggcc
      661 catgtgattc cgcgaccgca cgagggcctg accagactgt tgccggcgtg aagtcgtgcc
      721 ccatggtatc cgaactggac ggctttcatg accaggaaca cacttcccca acctgcaacc
      781 cggccggcag gcatccgaca cggcattgtc gtcttcctgg cggccttcat ggccatgctg
      841 cctgcactgc ggggcggttt catcgagggg gatgatgaac aacttgtcct caacttcgtt
      901 ctcgtcagcc ggccgtcatt gtcccacgcc ctcgagttgc tcactcttga accgcaccga
      961 tacctctacc aacccgttcc gatgctcagc ttcgcggcgg agttcgccct gctcaaagcc
     1021 gtcggcctca gtgccctgcc ggacgccccg aactccggca cctggctgtt ccacctgacc
     1081 aacgttctca tccacgccgc cgccgccctc cttgtgtggc ggctgatacg tcgtttgcat
     1141 cctgacccgc ttgtcgcgct ggtcgccggt attctgtttg cggttcatcc gatgggctcc
     1201 gagccgaccg cctgggtttg tggacggatg atctcgctgt cggcgctctt ctcactgctt
     1261 tcactgaatc tgcttgatgc ttgggaagaa cggccgcgca tcgggctggc tctgcttgtg
     1321 ctcgcagcgg tggtgctggc catgaccagc aaggtccagg tcggcttgcc cgtcctcatg
     1381 ctcctgctgc cgctggcccg taggcgctgg ccgcgccgta cctggtgggt catctggggc
     1441 ctatctctgc tgatcaccgg ctctttcgcc gtgctgaacg tgaccctgac aaaacagacc
     1501 catctgttgg aagtggccgc cgagcgattg gaaggcccgc gcagcgttcg cgtcctgctc
     1561 gctctgggtt ggtatctcag tcgggtcgtg tggccggcag gcctggcccc gtttcatcct
     1621 gcgaggtggg tggtcgcttg gtccgatccc gatgttctga ctgctgtgct gacgctcctt
     1681 gtggcgcttg ttctggtgac gatctccgtg agatggacgc gaatcggagt cctggggctg
     1741 atctggttcc tggcaaatgt tgccgccact ctgcctcttc tgccctcacg aagctcgatg
     1801 ttcgctgaac gttacgtcta catctccaac atagggcttt tctgggcaat cgcggcattc
     1861 cttgtgttcg gctatcggcg gtgggccgaa gcgagaccgt cggtgagatg gttttcaaga
     1921 gttgccgccg cgctggctgt tctcatgttg gtcatcacaa catggcaaac cgtcacggct
     1981 ttccgggacg atatccgcaa gaacctccga gtcgcagagg tatatcccaa catacccggc
     2041 tcatggacgc gactgggctt ggcctactac ggtgcgggtc aatatgagca ggctatcgag
     2101 gctgctaacg aggatatggc tcgacatcct gccgagacca gtgccagtgt ccatcgcgtc
     2161 atcgggatgg cttaccacaa gatgaaacgc atcgatgacg ccatcgaggc cctgcaaaaa
     2221 tcgctggaag ccgatccgca cagctatcgg acgcatcgtc acctcgccga ggtctacaga
     2281 gaggcagggc ggacggacga ggccatggcc cactatcgtc acgctctggc actggctccg
     2341 atgtacgagc cggcactgct cgggcttggc cagatgctcc aaaccgcagg tcaggcggac
     2401 gaggcggtgc ccctctatga gcgcgtgctg aaggacaacc cttttcatct gacggccaac
     2461 cttgcgatgg ccgagatcga gatcggccgc tcgcagtaca acctggccgc cgcccgcctg
     2521 cagaagttgc tctcgtatgc ccctgaatcc tggacggcct ggaccaacct gggggtgtgc
     2581 caggtcaacc tcggccagcc cggccgtgcc cttgaatctt accgaacggc actctccatc
     2641 aacccccagg ccgaagccgc ggctctcaat ctggccggtc ttctcatcag cagagggcag
     2701 ggtcccgagg ccgcaaggat ccttgttcgg gctctggccg acacatcaga ccgccagaca
     2761 ctctggaccg cttatgatct cctggtcgaa ggcggtcaac ttgcggcggg tgccgatctc
     2821 tgggccggct ctctgaggcg tgagcccgat gccgccgacc tcaagtgctc gctggcctgg
     2881 gcgctgctct tgcgacagca atcaaatgag tcgcgaaagc tgctgaacga atgtgcgggg
     2941 cggcttgaga atgatcccgt ttttgacgca gccctcgcca tgctggcttt gggcgacggc
     3001 gatccgagtc cggccgtcaa gtgggtcgaa tcggccgctc gggcagaaga aggtccgcgc
     3061 cgagagatgc tgccgcgcat gaaacaggcc gtgcggctct acggagaaag gcattcggac
     3121 cagccttggc cctactatct tgccgcgaga
//
LOCUS       DAOTPW010000390         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_24273_length_3139_cov_4.887808, whole genome shotgun sequence.
ACCESSION   DAOTPW010000390 DAOTPW010000000
VERSION     DAOTPW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24273_length_3139_cov_4.887808"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="P5316_21305"
     CDS             complement(<1..754)
                     /locus_tag="P5316_21305"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRR87551.1"
                     /translation="MTFEFATAARIIFGNGSLSEAGPIALSLLGNVRKGKVMLVSGVS
                     TSRPSRLTAILAKHDIEFVTLPVTGEPTVEMVRTAADRAVVSNCRIVISIGGGSAIDA
                     GKAIAAMITNGGNPTEFLEVIGQGKPLSQPPVPFIAIPTTAGTGAEVTRNAVLVSTPH
                     RLKVSLRSPLMLPRVALVDPELTYDLPPALTAATGLDALTQLIEAFVSVRANPLTDGF
                     CREGIPRIARSLREVCEDGECAIAREDMALASL"
     gene            complement(751..1068)
                     /locus_tag="P5316_21310"
     CDS             complement(751..1068)
                     /locus_tag="P5316_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRR87552.1"
                     /translation="MLIVAVHVHVKPECVEAFRQATIENASHSIRESGIARFDVIQQS
                     DDPTRFVLMEVYKTPEAPAKHKETAHYAKWRDTVADMMAEPRSSMKYTAVFPDEAGWE
                     IPK"
     gene            1373..2269
                     /locus_tag="P5316_21315"
     CDS             1373..2269
                     /locus_tag="P5316_21315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR87553.1"
                     /translation="MIVDCHTHIWESVEQLGRGFARGQARVRGELPLRAGIDAHRLAT
                     RPADKVFVLAYRSYYLEADIPNDYVAAYVRQQADRLIGFASVDPTRPQEAIQELRRAR
                     EELGMQGLNIWPAAQDFHPAATCAMRLYPEACRLGMPVMFHIDAAVMPASKMEYARPV
                     LIDEVAREFPEMRIIVAQLGYPWTDETIVLLAKHPNVFADISGLTKRPWHAYNALLSA
                     YQAGVMDRLLFGSNFPYASPAGCIEALYSINQFCHGTNLPTIPREQLRQIVERDALEI
                     LGIESPAPPVREPKTAVIRADD"
     gene            2342..2917
                     /locus_tag="P5316_21320"
     CDS             2342..2917
                     /locus_tag="P5316_21320"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HRR87554.1"
                     /translation="MGAIRSPRPVNLICGFISNDADLIRRAIRILTQYYGPTDAVSEI
                     WPFDDTDYYEPEMGTNLLRQFASFAELIDPGRLASIKVHTNEVEGRIGYECGLPRGQR
                     PVNLDPGYIELSKLVLATTKNGSHRIYMREGIYAESTLHYREGKWQPWPHTYPDYASG
                     RYDAFFEDARNRYKSKLESLGQTKRPEGGRL"
     gene            2914..>3139
                     /locus_tag="P5316_21325"
     CDS             2914..>3139
                     /locus_tag="P5316_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87555.1"
                     /translation="MISGPVLMAAGVSLLLAFLISVALTPLCRAWAVKRQFVDRPQDE
                     GHKEHERPTAFGGGIAITAGILVPMGGMLLA"
BASE COUNT          619 a          932 c          987 g          601 t
ORIGIN      
        1 agaggcttgc caaggccata tcctcgcgag cgattgcgca ctcgccatcc tcgcagacct
       61 cgcgaagcga gcgagcaatc cgcggaatgc cctctctgca gaatccgtcc gtaagcgggt
      121 ttgctcgcac cgagacgaac gcctcgataa gctgagtcag agcgtccagg ccggtggcgg
      181 cggtcagcgc cgggggcaga tcgtaagtca gttcaggatc gacaagagcc actcgcggca
      241 gcatcagtgg gcttctgagg ctcaccttca ggcgatgcgg ggtggacacc agcacggcgt
      301 tgcgtgtgac ctccgcgccg gtccccgccg tggtcggaat cgcgatgaac ggtaccggcg
      361 gctgcgacaa gggcttgccc tggccgatga cctcaagaaa ctcggtcggg tttcccccat
      421 tggtgatcat cgccgcaata gccttgccgg catcaatcgc gctaccgccg ccaatactga
      481 tcacgattcg gcagttcgat acgacggccc gatcggctgc ggtgcgtacc atctcgaccg
      541 tcggttcgcc ggtgacaggc agggtaacaa attcgatgtc atgcttggcc agaatggcgg
      601 tgagccggct cgggcggctg gtcgagacgc cggagacgag catcaccttg cccttgcgca
      661 cgtttcccaa caagctcagt gcaatcgggc ccgcttcgga aagcgatccg ttgccgaaga
      721 tgatccgcgc tgcggtggcg aattcaaagg tcatttcgga atctcccagc ccgcctcatc
      781 gggaaaaacg gcagtgtact tcatgctgga gcgaggctcg gccatcatgt cggcgacggt
      841 gtcgcgccac ttcgcgtagt gggcggtttc cttgtgcttg gcgggggcct cgggtgtctt
      901 gtagacctcc atgaggacga atcgcgtcgg gtcgtcgctc tgctggatga cgtcaaaacg
      961 ggcgatgccc gattcgcgaa tactgtgtga tgcgttctca atggtggctt gtcgaaaggc
     1021 ttcgacacat tcaggtttca cgtgaacatg gacagctacg atgagcattg gcggaactcc
     1081 cgaaagggcc gaaaagcatt ggccgtctgg aaccgatagg attctaccag cccaaagccg
     1141 cgaggcaatc gggcaaccgg gccgtgagac gtcggcacga acgcccgccc ggcatgcgtt
     1201 gcccgaaaac ggcaaaccgc atgcgagggc aggcggtctc aggccctgtg ggcgggccga
     1261 tcgacgtcaa ttgacccggc aagccggaac ggctataagg tggactcagg gagtgtatcc
     1321 tttcggcctc ggcaggcgcg ggtgaccggt gacgcggata cctccgtaag acatgatcgt
     1381 agactgtcat acccatatct gggagtcggt tgaacaactc ggccgtgggt ttgcccgcgg
     1441 gcaggctcgg gtgagagggg aattgcctct gcgcgccggg atagacgccc atcgcttggc
     1501 cacgcgcccg gcggacaagg tctttgttct ggcgtaccgg agctattatc ttgaggctga
     1561 cattccgaac gactacgtgg cagcgtacgt gcgtcagcag gcggacagac tcattggttt
     1621 tgccagcgtt gatcccaccc gtccacagga ggccattcaa gagctgagac gggctcggga
     1681 ggagctggga atgcagggtc tgaacatctg gccggcggca caggactttc acccggccgc
     1741 cacctgcgcg atgcggttat accccgaagc ctgtcgcttg gggatgccgg tgatgttcca
     1801 catcgacgcg gcggtgatgc ccgccagcaa gatggagtat gcccggccgg tcctcattga
     1861 tgaagtggcg cgcgagtttc ccgagatgcg gatcattgtg gcccagttgg ggtatccatg
     1921 gactgatgag acgatcgtac tgttggccaa gcacccgaac gtattcgctg acatcagcgg
     1981 actgaccaag aggccgtggc atgcttacaa cgcgctgttg tcggcctatc aggcaggcgt
     2041 catggaccgc ctgttgttcg gcagcaactt tccgtacgcc tcacctgccg gttgcatcga
     2101 agcgctgtac agcatcaacc agttctgtca cggcaccaat ttgccgacca tcccgcgaga
     2161 acagctccgc cagattgtcg agcgagatgc gctggagata ctggggatcg aatcgcccgc
     2221 gccgcccgta cgggagccga agaccgcggt catccgggct gatgactgat ccgttggccc
     2281 gcgttctcca caacctgcgg cgtcccgttg atttacgaat gcccgcgact acactgagga
     2341 catgggcgcg atccgttcac cacgacctgt gaaccttatc tgcgggttca tttccaacga
     2401 cgcggatctg atcaggagag cgatccgaat actcacacag tactacgggc cgaccgacgc
     2461 cgtcagcgaa atctggcctt ttgacgacac agattactac gaacccgaga tggggacgaa
     2521 cctcttgcgg cagttcgcct cctttgccga gctgatcgat cccggccgat tggcgagcat
     2581 caaggtgcac accaacgagg tggagggacg gatcggctac gaatgcggcc tgcctcgcgg
     2641 ccaaaggccg gtcaatctcg atcccgggta catcgagctg agcaaacttg ttctggcaac
     2701 gaccaagaac ggatctcatc ggatatacat gcgggagggc atttacgctg agtcgacgct
     2761 gcactatcgc gagggtaagt ggcaaccctg gccgcacacc tatcccgatt acgccagcgg
     2821 tcgctacgat gccttcttcg aggatgcacg caatcgctac aagagcaagc ttgaatccct
     2881 cgggcagacg aaacgaccgg aaggagggcg tttgtgattt ccggtcctgt tctcatggcg
     2941 gccggcgtat cgcttcttct ggcgttcctg atcagcgtcg ccctgacgcc cctttgtcgg
     3001 gcctgggccg tcaaacggca gttcgttgat cgcccgcaag atgagggaca caaagagcat
     3061 gagcggccaa cggctttcgg cggaggcatt gccatcacgg ccggaatcct cgtgccgatg
     3121 ggggggatgc ttctggccg
//
LOCUS       DAOTPW010000391         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_24355_length_3132_cov_4.043549, whole genome shotgun sequence.
ACCESSION   DAOTPW010000391 DAOTPW010000000
VERSION     DAOTPW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24355_length_3132_cov_4.043549"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..298
                     /locus_tag="P5316_21330"
     CDS             134..298
                     /locus_tag="P5316_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87556.1"
                     /translation="MDWDKTVNTVADVGRKTFTLALATVLVLLAVSGTYIVGLALWWL
                     VRYVRQALGT"
     gene            309..746
                     /locus_tag="P5316_21335"
     CDS             309..746
                     /locus_tag="P5316_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87557.1"
                     /translation="MPYPSHSQRRVPTARSTMGAEFAHADGQLDAQAHFAEVIGSSPE
                     ADVDKQTVHRKVELSCGCYWPDAEIAGVCAECAASGAASNVCKKHYVVCDCGTPCCWR
                     HSAPSDDGAKRLCSRCRLRAKNESLKTTVLNGLNAALRRIFAG"
     gene            821..2905
                     /locus_tag="P5316_21340"
     CDS             821..2905
                     /locus_tag="P5316_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87558.1"
                     /translation="MIQRRADALGARRDPDLAAHLALLDNDPTNAALAQLVACMLDEY
                     EEEQRLAPNWLAAPPALEDLYDLHRPPDFEIGQTTETGVRYGPRMRHDCGSIVIAGIS
                     GGGKTSAVQAIIVGTHRHLHQVATLVFDVKGDYTCIAAMRHPAVRVHKLREEVPLCLF
                     RPPVGVPLDAWLASVATYFCEYRGLKKSRHLFLDAARRLCKHFAVDRDPTKPWPSLFN
                     VLDYLRQLRGPKFGKEAEYRYSLINELQGLLEDTGTVFDTCDGIDVDQHMLAAGGATV
                     LQMETLPAPAQQMIISLTVERIIAKRVAANVHNPGLEVLIVLDEAQQILSRKADWEAS
                     NGIAPLASQLLRAREAGIGFIVVPHLLPDTSRAVLAAAKSMLVVGGLSDTASIDIAAQ
                     MMNLSPRAKTMIPRLGCGQALVREVGQGAYTDAFLVDVDPPVLAKDAISEAGRQRLMA
                     PKLAGLPRTTSKPLTNYPSIMAELHTPWTAPAPAASQTSTVPSVTQEMSDLLFDAARH
                     KGDWMVERRARLNIRDYKILQGLAQALEAQGLVRQHTIRLGRTTYALLEVTDHGWRIL
                     GQTKPVGYIGHGSLVHTVLISRVARYLASKTWANVQTEFPVGPTGHAVDVYGRSPAGL
                     VTAFEITLSTSNVVSNALRTLAPPSPVQELIFLCPVQKECLQVEALLRGDPLAAPHLA
                     HIRFRRIDEFLS"
BASE COUNT          545 a         1039 c         1001 g          547 t
ORIGIN      
        1 cgactcttcc gcgtcaagtt caccaggccc gacagcgccg gggttccggc aatcgcggct
       61 gtgcggcaat tggatgccga cgccggcacc ccgccacggc cgacgggcac gcccggtgat
      121 caggaggcca accgtggact gggataagac cgtcaacacg gtagccgacg tgggtcgaaa
      181 gaccttcacg ctcgcgctgg cgaccgtgct tgtccttttg gctgtcagcg gcacgtacat
      241 cgtcggcttg gccctgtggt ggctcgttcg gtacgtgcga caagccctgg gtacgtagag
      301 gatgcacgat gccttacccg tctcactccc agcgccgcgt tcccacagcc aggtcgacca
      361 tgggcgccga gtttgcccat gctgatgggc agctggacgc tcaggcgcac ttcgccgagg
      421 tgatcggctc ctcgccggag gcggatgtcg acaagcagac cgttcatcgc aaggtcgagc
      481 tatcctgcgg gtgctactgg cccgatgccg aaatagcggg cgtttgcgcg gagtgcgctg
      541 cttccggcgc tgcgtccaac gtgtgcaaga agcactacgt cgtttgcgac tgcggcacgc
      601 cctgctgttg gcggcattct gctccgtcgg atgacggggc caagcggctc tgcagccgct
      661 gccgattgcg cgcgaagaac gaatcgctca agacgacggt gctcaacggc ctcaacgcag
      721 cgctccgacg gatcttcgcg ggctgaagct cgactggccg aatcctgaga ccatgttcgg
      781 gaacccgcac cagcaccgtg agattctgcg agagatcggc ttgatccaaa gacgtgccga
      841 tgcgcttggc gcgcgccgcg atcctgacct cgccgcccac ctcgctctgc tcgacaacga
      901 tccaacaaac gccgcgctgg cgcagctcgt ggcctgcatg ctggacgagt acgaggagga
      961 acagcggctc gccccgaact ggctcgccgc gccccccgcg ttggaggacc tgtacgatct
     1021 ccaccgcccg ccggacttcg aaataggaca aacgaccgag actggcgtgc ggtacggccc
     1081 gcggatgcgc cacgattgcg ggagcatcgt gattgccggg atcagtggcg gcggaaagac
     1141 ctcggcggtg caggcgatca tcgtcggaac gcaccgacac ctgcaccagg tcgccacgct
     1201 cgttttcgat gtgaagggcg actacacgtg catagccgcg atgcggcatc cggccgtgcg
     1261 tgtacacaag ctgcgcgagg aggtgccgct gtgcctgttc cggccgccgg tcggagtgcc
     1321 gctggatgcg tggctggcca gcgtggccac ctacttctgc gagtaccggg gcctgaagaa
     1381 gtcgcgtcac ctcttcctcg acgccgcccg gcgcttgtgc aagcatttcg cggtcgaccg
     1441 ggacccgacg aaaccatggc cgagcctctt caatgtgctc gattacctca ggcaattgcg
     1501 cggcccgaag tttgggaaag aggccgagta ccggtacagc ctgatcaacg agcttcaagg
     1561 cttgctcgaa gataccggga ccgtcttcga cacctgcgac ggcatcgacg tcgaccaaca
     1621 catgctggcc gcgggcgggg ccacggttct gcagatggaa accctgcctg cacccgccca
     1681 gcagatgatt atcagcctca ccgtggagcg catcatcgcc aagcgcgtgg ccgcgaacgt
     1741 ccataatccc ggcctcgagg ttctgatcgt cctggacgag gcccagcaga ttctcagccg
     1801 gaaggccgac tgggaggctt ccaacgggat cgcgcccctg gcgagccagc tcctgcgggc
     1861 tcgggaagct ggcatcggct tcatcgtcgt gccccatctc ctgccggata catcccgcgc
     1921 ggttctggcc gcggcgaaga gcatgctggt ggtcggcggc ctcagcgata cggcgagcat
     1981 cgacattgcg gcgcagatga tgaacctctc gccacgggcg aagaccatga tcccgcggct
     2041 gggatgcggc caggcgctgg tccgagaagt cggccaaggc gcgtacaccg acgcgttcct
     2101 cgtcgacgtc gatcctccgg ttctggccaa ggacgccatt tccgaagccg gtcggcaacg
     2161 gctcatggct cccaagctgg ccgggttgcc aagaaccacc tcgaagccac tgacgaacta
     2221 cccctcgatc atggcggaat tgcacacgcc atggacggct cccgctccag ccgcgagcca
     2281 gacatcgacc gttccgtccg tcacccagga gatgagcgat ctcctgttcg atgccgcccg
     2341 gcacaagggc gactggatgg tggagcgtcg cgcgcggctg aacatccgcg attacaagat
     2401 cctccaggga ctggcgcagg cgcttgaggc gcagggcctc gttcggcagc acacgatccg
     2461 tctcggccgg accacttacg cgttattgga agtcactgac cacggatggc ggatcctggg
     2521 ccagactaag ccggtgggct acatcggtca cggaagcctg gtgcacaccg tcctgatcag
     2581 ccgcgttgcc cgctacctcg catccaagac gtgggcgaac gtccagaccg agtttccggt
     2641 cgggccgaca gggcacgccg tcgacgtgta cggccgcagc cccgccgggc tggtaacggc
     2701 cttcgagatc acgctgtcca cgtccaacgt ggtcagcaat gcgctgcgca cgctggcccc
     2761 gcccagcccc gtccaggagc tgatcttcct ctgtccggtg cagaaggagt gcctgcaagt
     2821 cgaggccctg ctccgcgggg accctctcgc cgcaccgcat ctcgcccaca ttcggttccg
     2881 ccgaatcgac gagttcttgt cctaggaaag tttctgtccc tattcctgca ggacaagtcc
     2941 atgcgcagga gaggcgacca tgcgggcggc aaaggctgtc ctggttatct ggatgctctg
     3001 cgcgattgcg ctgtgggtcc ggagcggtca gaacttctcg gtcttcgaca cgttgccgtt
     3061 cgtcgaacgg cgcgtggctc ttcccccgga ttatgactgg ttcgcgttgg cggtgctggc
     3121 aaccgggttg tg
//
LOCUS       DAOTPW010000392         3125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_24415_length_3125_cov_6.163192, whole genome shotgun sequence.
ACCESSION   DAOTPW010000392 DAOTPW010000000
VERSION     DAOTPW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24415_length_3125_cov_6.163192"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..837)
                     /locus_tag="P5316_21345"
     CDS             complement(271..837)
                     /locus_tag="P5316_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HRR87559.1"
                     /translation="MQDVIKGKAYVLGDNIDTDQIIPAHYLMYNPAIPEERKQFGRFA
                     LCGVPDAQAGLPQGHVPFVDQSDPNNTRSRFTIVIGGKNFGCGSSREHAPLALAEAGV
                     QAVVAPFYARIFFRNSVNGGYLIPLEFAEGVTHTVRTGDEVEIRLKENVLIECRSGTP
                     RALNPLGEIAPIVEAGGVFAYAKRTGML"
     gene            complement(1053..1913)
                     /locus_tag="P5316_21350"
     CDS             complement(1053..1913)
                     /locus_tag="P5316_21350"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRR87560.1"
                     /translation="MHYRSPITASIAAWSLSLALGCQAAKPTPFERASCSTLVKAKQP
                     LEPALKTVADLGFKYADVMCLSWTPHVNVDALVKDFDTEAARVEKTLADNKLKVSNLT
                     FDAIETKPFDEYKTRFTAVVKLAVRLRAPLINIMAPSAKSDWADQVAKLKVIHKIAAD
                     HGIKLTLETHTGQLTEYPADAEKICREVPGLGLTLDPSHYYAGPNQGKCFDSLYPYVM
                     NTGFRAGGMKREDIQMRWGEGPIDFTAIVRKLEAHGYRGYYVVEYLEGLGKADSVESS
                     RLFLEWIRGL"
     gene            complement(2054..2299)
                     /locus_tag="P5316_21355"
     CDS             complement(2054..2299)
                     /locus_tag="P5316_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87561.1"
                     /translation="MKVSHQEAKLMEHQGALDGDVDQADFGRFQACLSGAGAIQSNPE
                     CLAALIDDDGDVDGDDTQLFLGCLSGPDIPVSPQCAE"
     gene            2469..2687
                     /locus_tag="P5316_21360"
     CDS             2469..2687
                     /locus_tag="P5316_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87562.1"
                     /translation="MTIVNGHFDGQRVVLDEPVPSGVSANTPVKVVFDSEGADDVLAE
                     IARLGRPGGLPPDFAEQHEHYVKGTPRR"
     gene            2684..2881
                     /locus_tag="P5316_21365"
     CDS             2684..2881
                     /locus_tag="P5316_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87563.1"
                     /translation="MKTTFLDTSYLLALVLADDALHERALAWQKQVTGQLLTTEYALL
                     EFVDPLSEPRVRMPAVPDSSR"
     gene            2878..>3125
                     /locus_tag="P5316_21370"
     CDS             2878..>3125
                     /locus_tag="P5316_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87564.1"
                     /translation="MTAAQLDRGYVLLLPGVAKKPSSMAGIAGGLRDAGIDQAIERDL
                     RGSRPFGGLENVQLLERNREWADEGVGPTCGSAGPRFLS"
BASE COUNT          560 a          915 c         1025 g          625 t
ORIGIN      
        1 tggccgctct gcggcttggc ccctcgcttg cgctcggggt tctgaaagag ccgcccaagg
       61 cggaccgcct ttttgcgggc tgcgagccaa gcgctctgcg atcgagaaac atgcgcgcaa
      121 aatgcgcacc cgttgcgacg gaagggacta aaccgtcatg gacccctcgg tcacagccgg
      181 cctcatgtag ggcccggtgt gacgaaggtg ggggcagggc aagaggtgcg cggtcctttg
      241 ggcaacaagc tggtcggcgg cctgcgggta tcacagcatg ccggtgcgtt tggcgtaagc
      301 gaagacaccg ccggcctcga cgatgggggc gatctcgccg agtggattga gcgcccgggg
      361 ggtgccagac ctgcattcga tcagcacatt ttccttcaac cggatctcga cctcgtcgcc
      421 ggtgcggacc gtgtgggtca cgccttcggc gaattccagc ggaatgaggt aaccgccgtt
      481 gaccgaattg cggaagaaga ttcgggcgta gaacggggcg acgaccgctt ggactccggc
      541 ctcggccaag gccagcgggg cgtgctcgcg ggagctgccg cagccgaagt tcttgccgcc
      601 gatgacgatg gtgaaccggc tgcgggtgtt gttcgggtcg gactgatcca cgaagggaac
      661 atggccctgg ggcagaccgg cctgagcgtc gggcacgccg cagagggcaa accgcccgaa
      721 ctgctttcgc tcctccggaa tggcggggtt gtacatcagg tagtgcgccg gaatgatctg
      781 gtcggtgtcg atgttatcac cgagcacata ggccttgccc ttgatgacgt cttgcatggt
      841 atgcctcagc agaagctgtc agctattggc tttcggcttt cagccattcg ccctcagcaa
      901 atcggttgcc tgctattggc cggtggcctc agccaaacgg ccgaccgtat catgagacgg
      961 cccattatag ccgagcgcga gaggcaggaa agcgaatgct cgggaaccgg tgcaaacggc
     1021 tgcgagatgg aatcagcagg ccgtccggcg cttcacaggc ccctgatcca ctcgagaaac
     1081 agtcttgacg actccaccga gtcggccttg ccaaggcctt cgaggtactc gacgacatag
     1141 tagcccctgt aaccgtgggc ttcgagcttg cggacaatgg ccgtgaagtc aatgggtccc
     1201 tcgccccatc tcatctggat gtcttcccgc ttcattccgc cggcgcggaa gccggtgttc
     1261 atcacgtacg ggtacaggct gtcgaagcac ttgccctgat tcgggccggc gtagtagtgg
     1321 ctcgggtcga gggtgagtcc caggccggga acctccctgc agatcttctc ggcgtcggca
     1381 gggtactcgg tgagctggcc ggtgtgagtc tcgagggtga gcttgatgcc gtgatcggcg
     1441 gcgatcttgt ggatcacctt gagtttggcg acctggtcgg cccaatcact cttggccgac
     1501 ggggccatga tattgatcag cggggccctg agccggacgg ccagcttgac cacggccgtg
     1561 aatcgcgtct tgtactcatc gaacggcttg gtctcgatgg cgtcgaaggt caggttcgag
     1621 accttcagct tgttgtcggc cagagtcttc tccactcgcg cggcctcggt gtcaaagtcc
     1681 ttcaccagcg cgtccacgtt cacgtgcggt gtccacgaaa ggcacatgac gtcggcgtac
     1741 ttgaagccca ggtccgcgac ggtcttgaga gccggttcca gcggctgctt ggccttgacc
     1801 agcgtcgaac acgatgcccg ctcgaagggt gtcggctttg ccgcctggca cccgagggcc
     1861 agcgacagcg accatgcggc aatggaagcg gtgataggcg atcgatagtg catgtgtgac
     1921 ctcctgcaag ttgttggttc tgtcttgctg agtctgcagg ccatgctaat cgcgggcttg
     1981 ctgatcgtcc agaacaccgc ttgcgcgcgg cgtctgcgcg tgtcgccgag aacctgggtc
     2041 ggggactacc gattcattca gcgcactgtg ggctcaccgg aatgtccggg ccgctgaggc
     2101 agccaaggaa gagttgagta tcgtctccgt cgacgtcgcc gtcgtcatcg ataagggcag
     2161 cgagacactc cgggttactc tggatggcgc cggcgccgct caggcaggcc tggaagcggc
     2221 cgaagtccgc ctggtcaacg tcgccgtcga gagcaccctg atgctccatg agcttggctt
     2281 cttgatgact aaccttcata ccccagtccc tgccggtctc ttgcacgccg gtgaccttgg
     2341 tcctcgccca tgtaaaaccg caccccgttt gtcgtataat agtcatattg aagcagccgc
     2401 cgagttcggg caatgggatt aatcagaccc ggcattgacg gctgtgcctg agcgaacgga
     2461 gagacgagat gaccattgtc aatggacatt ttgacggtca gcgagtcgtt cttgacgaac
     2521 ccgtgccatc gggtgtcagt gccaatacgc ccgtgaaggt ggtgttcgac agcgaggggg
     2581 cggatgacgt cctggcggag attgcccgtc ttggacgccc cggtggactg cctcccgatt
     2641 tcgccgagca gcacgaacac tacgttaagg gaacaccccg gcggtgaaaa ccacattcct
     2701 cgatacctcg tacctgcttg cgctcgtatt ggcggatgat gctcttcatg agcgagcact
     2761 ggcttggcag aagcaggtaa ccggacaact gttgaccact gagtatgctc tcctggaatt
     2821 tgtggatcct ctgagtgaac caagagtgcg gatgccggct gtgcccgatt cgagccggtg
     2881 acggcggcgc agttggatcg cggctatgtc ctgttgctgc cgggcgtggc caaaaagccg
     2941 tcgtcgatgg cgggcattgc cggcggactc agggacgcgg gaattgacca ggccatcgag
     3001 cgggatctgc ggggctcccg gccgttcggc ggactcgaga acgtgcagtt gctcgaacgg
     3061 aatcgagaat gggccgacga gggcgtcggc cctacttgcg ggtccgctgg tcccagattc
     3121 ctatc
//
LOCUS       DAOTPW010000393         3110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_24574_length_3110_cov_5.494926, whole genome shotgun sequence.
ACCESSION   DAOTPW010000393 DAOTPW010000000
VERSION     DAOTPW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24574_length_3110_cov_5.494926"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="P5316_21375"
     CDS             <1..578
                     /locus_tag="P5316_21375"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRR87565.1"
                     /translation="GVPGKPSKTYIFGAEESYGYMPCTYTRDKDGVTSACCIADLAAV
                     TAARGKNLYDLLQELYQRFGYFQEGAKSLELPGADGAARISDMMQRLRTQPPASIGGI
                     PVVTIADLTTGEIRDARTGKPVGRYDLPAADVVFFTLEDGTKVVVRPSGTEPKIKFYV
                     LTRSPGDDLPKARADATARIKAIIDDITNQA"
     gene            complement(621..2111)
                     /locus_tag="P5316_21380"
     CDS             complement(621..2111)
                     /locus_tag="P5316_21380"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRR87566.1"
                     /translation="MRRCENVMMRTESSRTFGEPGLPGDRRFGPAGRCTGTVALLGLA
                     TILATQCGCPVPQRPGLGQCTRLVEPETKTGYWLYLPEDYVKNNGQRPGNKPWPLVVT
                     LHGLRPYDDARPQIRSWQEEADRYGLIVVAPELRTCDQLVMPLPLRDPTKWYVQTDEK
                     AILAVMDEVCRRTNADPTRVLLTCFSSGGYLAHYMMNRHPERFTALAAMGANFNEEMI
                     DYTQLPKYRSAKIAIFFGQNDFKLCRDESQRAVSWYRRYRFDVDARQVSGLGHERRPQ
                     LASVFFSRIIGASPKTPPDLASLVMSDVTTGEPARPAGAPRPAPLSPAPLFPTDVGRA
                     SPGSERPQDTGSEAQGREPATVAPIEAPTRSARPVVNQPPPPTPRRPRLQPYSAEPQI
                     PVPVPDQGPEPIPGRIEIRGLSTGSTPMWVDMRIELPPDLLPEAGILWLADGRPIASS
                     TPEARAILRTPGTHHIEAWVTLADDRHAIYRETITVPHSQSQPADG"
     gene            complement(2385..>3110)
                     /locus_tag="P5316_21385"
     CDS             complement(2385..>3110)
                     /locus_tag="P5316_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87567.1"
                     /translation="LVGILQVSALNTAVKQEEGSLAWVGMFLTIMFALEGAYFGSVAY
                     YCLIDTIRSTVGGNEHPPPLTWNITKLGAALGGYVALIGFYLVVILVLVTAYGGVPTS
                     LADFSVLGKPLSLAVLAIMTFSIPMNIIGLASTEPLDGLNPAKVGLSILRVIGHYIFM
                     FLIVILYVGFYVGILYAIMSWAGPAMKQAAAQGIEVGYKNLLGGIAGWAVLIGMGFYF
                     AYMIGRILGLFARTYRERMAFEL"
BASE COUNT          572 a          953 c         1031 g          554 t
ORIGIN      
        1 agggggttcc aggcaagccc agcaagacgt acatctttgg tgccgaagag agttacggtt
       61 acatgccgtg cacctatacg cgagacaagg atggggtcac cagcgcctgc tgcatcgccg
      121 acctggcggc cgtcactgcc gctcgcggca agaatctcta cgacctgttg caggaactct
      181 accagcggtt cggttatttc caggaggggg ccaaaagctt ggagttgccc ggggccgacg
      241 gtgccgcacg tattagcgac atgatgcagc gattgcgcac gcaaccgccc gcgtcgatcg
      301 gcgggatccc ggtggtcacc atcgcggacc tgaccacggg agaaatccgc gacgcccgca
      361 ccggcaagcc cgtcggtcgc tatgacctgc ccgccgccga cgtggtcttc ttcaccctcg
      421 aagacggcac caaggtggtt gtccgcccca gcggtaccga gccgaagatc aagttttatg
      481 ttctgacccg cagccccggt gacgacctgc ccaaggcccg tgccgacgcg acggcccgaa
      541 tcaaggccat catcgacgac atcaccaacc aggcatgagt agatccatcc tgatgggcag
      601 gtgcaggcag tcagctccta ctagccgtca gcaggttgtg attgcgagtg cgggacggta
      661 atggtttcgc ggtagatggc gtgtcggtcg tcggcaagcg tgacccaggc ctctatgtga
      721 tgcgtaccag gggttcggag gatggcgcgg gcctcgggcg tgctggaggc gatgggccgt
      781 ccgtcggcca gccagaggat gccggcctcc ggtagcagat caggcggaag ctcgattcgc
      841 atgtcgaccc acatgggggt cgatccagtg ctcaggcccc gaatctcgat tcggccgggg
      901 atcggctcgg gtccctgatc gggcacaggc acaggaatct ggggctcggc cgaatacggt
      961 tgcagtcttg ggcgtcgcgg cgtcgggggg ggcggctggt tgacgaccgg ccgggcggat
     1021 cttgtgggcg cttcgatcgg cgcaactgtg gccggctccc gaccttgagc ctcagagccg
     1081 gtgtcctgcg gtcgctctga gccgggactc gcacggccca cgtcggtggg gaacagcggg
     1141 gcgggtgaaa gcggagccgg gcggggtgcc ccggccggac gggccggttc gcctgtggtg
     1201 acgtcgctca tgaccagact ggccaggtcc ggaggcgtct tcggcgaggc accgatgatc
     1261 cgggaaaaga acacgctggc cagttgcggc cttcgttcgt ggcccagccc cgagacttgg
     1321 cgggcgtcca cgtcgaatcg atagcggcga taccagctta ccgcccgctg gctctcgtcg
     1381 cggcaaagct tgaagtcgtt ctggccgaaa aagatggcga tcttggccga gcggtatttc
     1441 ggtagctggg tgtagtcgat catttcctcg ttgaagttgg cgcccatcgc ggccaaggca
     1501 gtgaaccgct cagggtgacg gttcatcatg tagtgggcaa ggtagccgcc ggacgaaaag
     1561 caggtgagaa gcactcgcgt ggggtcggca ttcgtccgcc ggcagacttc gtccatcacc
     1621 gcgaggatgg ccttttcatc ggtctgcacg taccacttgg ttggatcacg gaggggcagg
     1681 ggcatgacca actgatcgca ggtccgcagc tcgggtgcca cgacgatcag accatagcga
     1741 tcggcctcct cctgccatga tcggatctga ggccgggcat cgtcgtaggg ccgcagcccg
     1801 tggagcgtga cgaccagtgg ccacggcttg ttgccgggac gctgcccgtt gttcttgacg
     1861 tagtcttcgg ggaggtacag ccagtagccg gtcttagtct caggctcgac gaggcgggta
     1921 cactggccca acccggggcg ctgcggcacg gggcagccgc actgggtggc gagaatcgtg
     1981 gccaggccca ggagggcgac cgtgccagtg cagcggccgg ccggtccgaa ccggcggtcc
     2041 cccggcaacc caggctctcc aaaggtccga ctcgactccg ttctcatcat gacgttctca
     2101 caccgtctca tgggtcgttt gagcatcctg acttattcca tcatagtcag tttatcggcc
     2161 gaaaaggggg cgatcgacaa ctcctgaggg acgggcatgg ccgaccccgg tttactgccg
     2221 atgctggcgg cgggaaagcc cctcgaccct ggccaagccg cccgtggcgc ctggcaccct
     2281 ctgttgaacc gattgtcccc acctcaaatg aggtccgagg cggtgctcct cggttccgtt
     2341 acctgtcgtc cccatgtgcc tcagcccacc ccgtccccgt gacgtcacag ctcgaacgcc
     2401 attctctccc tgtaggtcct ggcgaaaagc ccgagaatcc gcccgatcat gtaggcgaaa
     2461 tagaagccca taccgatgag taccgcccag ccggcgattc cgcccagaag gttcttgtag
     2521 ccgacctcga taccctgcgc tgcggcctgt ttcattgccg gaccggccca gctcatgatc
     2581 gcatacaaga tcccgacgta gaacccgaca tagaggatca cgatcaggaa catgaaaatg
     2641 tagtgcccga tcacgcggag gatcgagagg ccgacttttg ccggattcag gccgtcgaga
     2701 ggttccgtgc ttgccagacc aatgatgttc atcgggatgc tgaaagtcat gatggccagg
     2761 accgcaaggc tgagcggttt gcccagcacg gagaaatcgg cgaggcttgt cggcacgccg
     2821 ccgtaggccg tcaccagaac caggatgacc acaagataga agccgatcag ggcgacgtac
     2881 ccgcccagag cggcaccaag cttggtgatg ttccaggtaa gcgggggtgg gtgttcgttg
     2941 ccgccgacgg tgctccggat cgtgtcgatg aggcaatagt aggccaccga gccgaagtag
     3001 gcgccttcga gcgcgaacat gatggtcagg aacatgccga cccaggcgag gctcccctct
     3061 tcctgtttga cggcggtgtt gagcgccgag acctggagga tgccgaccag
//
LOCUS       DAOTPW010000394         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_25421_length_3027_cov_4.579071, whole genome shotgun sequence.
ACCESSION   DAOTPW010000394 DAOTPW010000000
VERSION     DAOTPW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25421_length_3027_cov_4.579071"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..1003
                     /locus_tag="P5316_21390"
     CDS             317..1003
                     /locus_tag="P5316_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87568.1"
                     /translation="MTRAMKCFCLLMAAGLWACGCDDSSEHEATPALPPEHVAPPPSA
                     TDPEPPPRESPDSVEPDILISPKTRSATKPSSAGTRPERTEPFQPLADARTGEWALYE
                     ALDSQTLLYRVKEVGITRVKTEVRVTLDGRPLGMPAEREDLRTADPLAWDRPSDGHRQ
                     TSRTTIQAGGRDWDAVLYEDRWTEEGVKYVRRTWVSPAVPVFGIVRMELTGDGTVEAR
                     LELTEAGQTE"
     gene            complement(1061..>3027)
                     /gene="yidC"
                     /locus_tag="P5316_21395"
     CDS             complement(1061..>3027)
                     /gene="yidC"
                     /locus_tag="P5316_21395"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRR87569.1"
                     /translation="RPAPLSTAPSAPTPEAFPASGPASDKAVISRGSDTRPLVLGAAS
                     ADSPFPMEITVHPSGAVVSKVRLRGHYQTVEKREPYSLVEPVELVDKSGRLRVLNSFP
                     TTLRVLNPALDVPLDEAVWKVESYTEQQVVFSVDVSTPDGKPLLRVLKTYQLAPQEAK
                     SRTSDAQMSLQFENRYGQPVQIIVTQQGPIGFRKEDLRGEDRKVIGAVWRNGAVASKL
                     HPRADVIKRTQIELGSDINEGSRIAWVAEGNKYFGCIMAPSGRDHGDVPSRFTRAEAI
                     VFAPQVKDDAQVERQDLAFRFITEPISIPTGGLSGLAFDLYMGPKSKRIFESVETYAR
                     RSYYQMLSGEFAWCTPGWLVGLMMVLLNGVHAIWPHNYGLSIIVLVLVVKAILHPLSV
                     KGQINMQKMQKSQARLKPKMDAIKEKYANDRARMNQAMAEVMKEEGINPAGQMLTCLP
                     MMIQIPIWVALWTALSYTVEMRHAPLDGWWIKDLTQPDQFMRLFNRPVNVPLLGWLLG
                     GPVEHLNVLPILLAITQVLQTKFMPRGNTSSDRSPDQLEQQRKMMMFMSVFFMFILYN
                     APSGLTLYIMASNIFGIIEQWRIRRHIAELEARSEAAKAHPGPLRRLLSNAFGGDGRR
                     KSWLQAKWQELQKQIEQAKRVQSQRSKGRR"
BASE COUNT          596 a          889 c          978 g          564 t
ORIGIN      
        1 gttgcggccc gcaaaaaggc ggtccgcctc ggggccattc cgtcaggacc gtggttgctc
       61 accacggcga ggcggacctg cgacagcagg accgttaagg gagcggtccc tggaagatcg
      121 cagcaaacaa acgaagaacg gagcaaaccg cacagcaacc acgccgcaaa ggggttgcgt
      181 ccacggggtc gcggttctga acagggggct gaaagaacgg tcaggcgtct tcggcaagtt
      241 tctgacgaca cacggagaca ctgacgcagg cggcgttcca gttgcctgaa gggacctcac
      301 ggattacaag gttggcatga cgcgtgcgat gaagtgcttc tgcctgctga tggcggccgg
      361 cctgtgggct tgcggctgtg acgattccag cgaacacgag gcaacacccg ccctgccgcc
      421 cgagcacgtc gccccgcctc cgtccgcaac cgaccccgag ccgccgccgc gggagagccc
      481 tgactcggtc gaacccgaca tcctgatctc gccaaagacg cgatcggcga ccaagccgtc
      541 ttctgcgggc acccggccgg agcgcacaga gccctttcag ccgctggccg acgccagaac
      601 aggcgagtgg gcgctctacg aggcgctgga ttcccagacg ctgctgtatc gggttaagga
      661 agtgggcatt acacgggtga agactgaggt gcgggtgacg ctggacggac gcccgctcgg
      721 catgccggcc gaacgcgaag atttgcgaac cgccgatccg ttggcgtggg accgcccttc
      781 cgatgggcac cggcagacaa gccggacgac gattcaagcg gggggacgag attgggacgc
      841 cgtcctctat gaggaccggt ggacggagga gggggtgaag tacgtccggc gaacatgggt
      901 cagccccgca gtaccggtgt tcggtatcgt ccggatggag ctgacaggcg acggcaccgt
      961 cgaagctcga ctcgaactca ctgaggccgg acagacggag tgagtgccgg cgggcgagcc
     1021 gccagtgaca cggcagagag caaaagccgc ctgcattctg tcatcttctt cctttgctcc
     1081 tctggctctg gacgcgtttg gcttgttcga tttgtttctg aagttcctgc cacttggcct
     1141 gcaaccagga tttgcgccgg ccatccccac cgaaagcgtt ggacaagagc cgcctgaggg
     1201 gacccggatg tgcttttgcg gcttcgctgc gggcttcgag ctcggcgatg tgccggcgaa
     1261 tacgccactg ttcgatgatg ccaaagatat tgctggccat gatatacaac gtcaggccgc
     1321 tgggagcgtt gtagaggatg aacatgaaaa acacgctcat gaacatcatc atcttgcgct
     1381 gctgttcaag ctggtcgggg cttcgatcgc tcgaggtgtt gccccgcggc atgaatttgg
     1441 tctgcaggac ctgagtaata gccaacagga tcggaaggac attgaggtgc tcgacgggcc
     1501 cgccgaggag ccagccgagc aggggtacgt ttaccggccg attgaagagt ctcatgaact
     1561 ggtccggttg ggtaagatcc ttgatccacc atccgtcgag cggggcgtgg cgcatttcga
     1621 ccgtgtagct caaggcggtc cagagggcaa cccagatggg aatctggatc atcatgggca
     1681 ggcaggtaag catctggccg gcggggttga tcccttcctc cttcatgact tccgccatgg
     1741 cctggttcat acgggcgcgg tcgttggcgt atttctcctt aatggcgtcc atcttcggct
     1801 tgagccgggc ctggctcttc tgcattttct gcatattgat ctggcccttg accgaaagcg
     1861 gatggagaat ggccttgacc accagcacca ggacgatgat ggagagaccg tagttgtgcg
     1921 gccagatagc atggacgccg ttcagcagaa ccatcatcag gccgacgagc cagcccggtg
     1981 tgcaccaggc aaactcgccg ctgagcatct ggtaataaga gcgccgggcg taggtctcca
     2041 ccgactcgaa gatgcgtttg gatttcggcc ccatgtagag atcgaaggcc aggccgctta
     2101 agcctcccgt cggaatgctg atcggctcgg tgatgaagcg gaaagccagg tcctgccgtt
     2161 cgacctgcgc gtcatcctta acctgggggg caaaaacaat cgcctcggct cgcgtgaatc
     2221 gcgacggaac atcgccatgg tcgcgcccgc ttggcgccat gatgcagccg aaatacttgt
     2281 tgccttcggc gacccatgcg atccggcttc cctcgttgat gtcagaaccc aactcgatct
     2341 gagtacgctt gatcacgtcg gcgcgggggt gaagcttcga cgccacagcg ccgtttcgcc
     2401 acaccgcacc aatgacctta cggtcctcgc ctcgcaggtc ctctttccgg aaaccaatcg
     2461 gcccctgctg cgtgacgatg atctggaccg gttggccata gcggttctcg aattggagcg
     2521 acatttgcgc atccgaggtt cgtgatttgg cctcctgggg ggcgagctga taagtcttca
     2581 atacacgaag gagcggcttg ccgtcgggcg tgctgacgtc gacgctgaac accacctgct
     2641 gttcggtgta agactcgacc ttccagacgg cctcatcaag gggtacgtcg agagcaggat
     2701 tcagcactcg caacgtcgtc gggaaggaat tgagcacgcg caggcggccc gacttgtcaa
     2761 ccagttcgac aggctccacc agggaatagg gttccctttt ctcaaccgtc tggtaatgac
     2821 ctcgaaggcg gacctttgaa acgacggctc cagacggatg gacggttatc tccatcggga
     2881 aggggctgtc ggcggatgcg gcaccgagaa ccagcggccg ggtatcggat ccccggctga
     2941 ttacagcctt gtcggacgcc ggcccacttg cgggaaaagc ctcgggagtt ggagcggacg
     3001 gagcggtgct gaggggcgcg ggccggc
//
LOCUS       DAOTPW010000395         2970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_26027_length_2970_cov_6.185592, whole genome shotgun sequence.
ACCESSION   DAOTPW010000395 DAOTPW010000000
VERSION     DAOTPW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26027_length_2970_cov_6.185592"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="P5316_21400"
     CDS             <1..186
                     /locus_tag="P5316_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87570.1"
                     /translation="PGRADTFGGACPRKAVGMVPGKAVGMVPGKAVGMAPGKAVGMAP
                     GALRSRDMGASRTGIRP"
     gene            complement(341..1084)
                     /locus_tag="P5316_21405"
     CDS             complement(341..1084)
                     /locus_tag="P5316_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87571.1"
                     /translation="MKALFCLVVPLVLAANCIPAPEPPVEVSSFELEIQPEIVQPVIP
                     GQHCVLLMQVVNETAGTGHGEAVTLTATAPGATVTVEPTSITPAQVAEVTVIPEEASV
                     GQELTVTVTGWRSGKQATATKTLTVEEGDIDEFLNNLGPTAGEMRDMFIPWLETNHPE
                     LGITGQTEWAGVVVSPYILVVSHFLFFSDEWEMHVYWHVMIPPYDWVKIDLRNRFAEI
                     SYSYSFEISSRSDPEQQPHAIEPPEELWR"
     gene            complement(1135..1590)
                     /locus_tag="P5316_21410"
     CDS             complement(1135..1590)
                     /locus_tag="P5316_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87572.1"
                     /translation="MNRMWFRVTASTILLVATVMCAAPGKPRGGRTAGNEQCLECHPT
                     FRNETIAETHEQTGTLCVDCHGPSKAHAVDPKEKARPDIVFSRDQVDAHCGQCHDPAH
                     HPQRKAKRFMEQYEGHPGPNGRMITAGSICTDCHGRHIMPPPQASPAGS"
     gene            complement(1984..2754)
                     /locus_tag="P5316_21415"
     CDS             complement(1984..2754)
                     /locus_tag="P5316_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRR87573.1"
                     /translation="MPEPPLDPRLELGLDRRRLPRHIAIIMDGNGRWANQRNLPRIRG
                     HIEGAANVRETITYCARLQIEALTLYSFSAENWKRPPDEVNALMDLYADYLVKERPTI
                     MENNVRLIQIGRRQGLPDKVLAELDITQQMSAANTGLKLCLAINYSSRNEIVDAVRAV
                     ARRVQTGELRPEDIDEATIANSLYTTGVPDPDLLIRTAGEMRVSNFLLWQISYTELYV
                     CDVLWPDFKKQHLADAIRAFAARERRYGAVTRDISNHR"
BASE COUNT          540 a          803 c         1029 g          598 t
ORIGIN      
        1 ccgggccgag cggatacatt tggaggcgca tgcccacgca aagccgtggg catggtaccc
       61 ggcaaagccg tgggcatggt acccggcaaa gccgtgggca tggcacccgg caaagccgtg
      121 ggcatggcac ccggcgcact gcgatcaaga gacatgggcg caagccgcac aggtattcgg
      181 ccgtaaggtt ctaacgacaa gccggaagcc tgccccgtac ggaccgtcgt ctgacacata
      241 cagctcgatg ccctacgcaa gctgttgacc ggaaaaacct accatccgac aagggctgtt
      301 gatcgcacac ggcccactaa gaacgcgagc gaagggggcc ttatcgccag agctcttccg
      361 gcggttcgat cgcatgcggt tgttgttccg ggtcgctgcg agaactgatc tcaaaggaat
      421 aggagtagct gatctcggcg aagcggttcc gcaggtcgat tttcacccag tcgtagggcg
      481 ggatcatcac gtgccagtaa acgtgcattt cccattcatc cgagaagaac aggaagtggg
      541 agacgacgag aatgtacgga gagacgacca cgccggccca ttcggtctgg ccggtgatcc
      601 ccagctcggg gtgattcgtt tccagccatg ggatgaacat gtcgcgcatt tccccggccg
      661 tgggcccaag attgttcagg aattcgtcga tgtcaccttc ttcgaccgtc agcgttttgg
      721 tggcggttgc ctgcttgccg gagcgccagc cggtgacggt tacggtcagc tcctgtccca
      781 cggaggcctc ttccggaatg accgtgacct cggccacctg ggcgggggta atcgaggtgg
      841 gctcgacggt gacggtggcg ccgggcgccg tggcggtcag ggtgacagcc tcaccgtgtc
      901 ccgtgcctgc ggtttcgttg accacttgca tgagcagcac gcaatgctgc cctgggatga
      961 cgggctggac gatttcaggt tgaatctcca gttcgaaaga tgagacttcg acgggcggtt
     1021 ccggagccgg gatgcaattc gcggccagaa caaggggcac gacgaggcag aaaagagctt
     1081 tcatggcgtg ctacctttct ccatgcaggt tgcaccaaca gtgacggacg tttttcagct
     1141 tccggccggg gaagcctgcg gcgggggcat gatgtgtcgg ccgtggcagt cggtgcagat
     1201 gctgcccgcc gtgatcatgc ggccgttggg cccgggatgg ccttcatatt gctccataaa
     1261 acgttttgct ttgcgctgcg ggtggtgcgc cggatcatgg cactgtccgc agtgggcgtc
     1321 gacctggtcg cgggagaaga cgatgtcggg tcgggctttt tccttgggat cgacggcatg
     1381 agcctttgag ggcccgtggc agtcgacgca gagcgtgccg gtctgctcgt gggtttcagc
     1441 aatcgtttcg ttgcggaatg ttggatggca ttcgaggcac tgttcattgc cggcggtccg
     1501 gccgccacgt ggttttccgg gtgccgcaca cattacggtg gcaacgagca ggatggttga
     1561 agccgtgaca cgaaaccaca ttctattcat gagctgcctc ctgatcggtc gggaaagcca
     1621 ttgagtaccc tgcctgtctg agggggcgag caggcgcggc attcacgagt ggtggattat
     1681 agcgggcagg catggcgggt gaaaggtcgc gggagaatct gaggaacatg aggaatgacc
     1741 aaggttcaat ggcggtcaaa tccgaatgac gaagcccgac tgacgaagca gccccgaaac
     1801 ccggatgacg cagtccacgg tggcgggcag gtttgcccag aggggcatcg ttattatcca
     1861 gtccgaactt cgtcattcag cggttgttgg ggcttgggaa ttggacatgc cactcggatt
     1921 gcgagccgga cctgcgacgg gggcttgtca tgtcgaaggt gtgactcggc ggcgtggaag
     1981 ggttcagcgg tggttggaga tgtcgcgggt gacggcgcca tagcggcgtt cgcgggcggc
     2041 gaaagcgcgg atggcatcgg cgaggtgttg ctttttgaag tcgggccaga ggacgtcgca
     2101 gacatagagt tcggtataac tgatctgcca gagcagaaag ttgctgactc gcatctcgcc
     2161 ggcggtgcgg atgagcaggt cggggtcggg cacgccggtg gtgtaaagcg agttggcgat
     2221 cgtggcttcg tcgatatcct cgggcctcaa ttcgccggtc tggaccctgc gggcgacggc
     2281 ccggaccgcg tcgacgatct cgttgcgcga gctgtagttg atcgccaggc agagcttaag
     2341 gccggtattg gcggcactca tctgctgggt gatatccagc tcggccagca ccttgtccgg
     2401 cagtccctgg cggcgcccga tctgtatcag gcggacgttg ttttccatga tcgtgggtcg
     2461 ttccttgacg aggtagtccg catagaggtc catgagggcg ttgacctcgt cgggagggcg
     2521 tttccagttt tcggcgctga agctgtagag cgtcagggcc tcgatctgga ggcgtgcgca
     2581 gtaggtaatg gtctcgcgga cgttggcagc accttcgatg tggccgcgga tgcgggggag
     2641 gtttcgctgg ttggcccacc ggccgttacc gtccatgatg atggcgatgt ggcggggaag
     2701 ccggcggcgg tcgaggccca gttccaatcg cgggtccaat ggcggctcag gcatcatctt
     2761 cttccaggta ccgctcgcct cggaattcct tccgggtcgc ctcggatggc ggaatccgtt
     2821 tcgccttccg tgacgatgag gatgggaatg ctccgcgcag aaggccccag aacggattct
     2881 ggggccagca aactgctgat ggcttacccc gttcgcgaag cgcgaaaggg acggaggaag
     2941 ccgtcggttc aaggccgagt tcaagccccg
//
LOCUS       DAOTPW010000396         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_26265_length_2948_cov_5.350156, whole genome shotgun sequence.
ACCESSION   DAOTPW010000396 DAOTPW010000000
VERSION     DAOTPW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26265_length_2948_cov_5.350156"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="P5316_21420"
     CDS             <1..720
                     /locus_tag="P5316_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87574.1"
                     /translation="SITPDVHEHPGTGELFAVHYCSKEGYGRITVSVDQMRQWQSDPD
                     GMAKWLCAQLALASAPTSIVRGQLYLLGTRPTTNGPLDVFLAFGLSQASGYDVVVQNV
                     ERLRATPGSVIIMPAYMPVPGIWSILKANWLILTDLVLWDAERSVLDASMLVRAVQAA
                     CPAISNERWITVTEGARLLTNDLPYLDMKKAAARISKAAGAGKFMTNGKARDARRIDR
                     TSFDAWRLEQRDRDLDAEEDD"
     gene            1018..1569
                     /locus_tag="P5316_21425"
     CDS             1018..1569
                     /locus_tag="P5316_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87575.1"
                     /translation="MNASSPQRLPKFRAHKATLQAYVELSGKRFYLGCHESAEARQKY
                     NTLVAEWLANGRQLRANPEQITDKELTTRYWVYGAQHYRRADGTVARELDNIVDALRP
                     AKELYGTKPTAQLGPRALRTIRQKMIQSGRRRRNINCRIGRIKCVFKWAAGDELIPGE
                     VYHALPAVESLRGGQSQAIENGS"
     gene            complement(1677..2243)
                     /locus_tag="P5316_21430"
     CDS             complement(1677..2243)
                     /locus_tag="P5316_21430"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRR87576.1"
                     /translation="MVSPEFARVVKMFWLLVVVLLLLNAAWLILVALGLPGTWLMVIT
                     TALVAWWKWPADSTVDPPMIGIGVLAAIMVLAALAEVLELVAGVLGAKATGAGRRGSI
                     GALAGGLIGALVGTFLIPIPLLGSLLGACVGAGLGAWGLELSGGRRQLAALKAGIGAG
                     VGRFVGTVVKLTAGVLIWLVVAIAAFWP"
     gene            2689..>2948
                     /locus_tag="P5316_21435"
     CDS             2689..>2948
                     /locus_tag="P5316_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87577.1"
                     /translation="MESQKSGSAQQDFYILQDANFNVMALTDAGGGVVEQYQWEPYGL
                     LAAKDTDTGGTIPHSHIGHQGLFFYRFNPQTGDTATLSTTAVG"
BASE COUNT          630 a         1039 c          833 g          446 t
ORIGIN      
        1 tcgattacgc ccgacgtgca cgagcatccg gggacgggtg agctgttcgc cgtgcactac
       61 tgctccaagg agggttacgg ccggatcacc gtatcggtgg accagatgcg gcagtggcag
      121 tcggaccccg acggcatggc gaagtggttg tgcgcgcaac tcgcgctggc gtccgcgccc
      181 acatcgatcg tccgagggca gctctacctg ttgggtacgc gtccgacgac caacgggccc
      241 ctggacgtct ttctcgcctt cgggctgagt caagcttccg ggtacgacgt ggttgtccag
      301 aacgtggagc gcctgcgggc gacaccgggg tcagtcatca tcatgcccgc ctacatgccg
      361 gtacccggga tctggtcgat tcttaaggcg aactggctaa tcttgaccga tctcgtgctg
      421 tgggatgcgg agcgaagcgt tctggatgcg tcgatgcttg tgagggccgt gcaggcggcc
      481 tgcccggcga tctccaacga gcggtggatc accgtcacgg agggcgcccg gctgctcacg
      541 aacgacttgc cgtatctcga catgaagaag gccgccgccc gaatctccaa ggccgccggc
      601 gccggcaagt tcatgaccaa cggcaaggcc cgcgatgcgc gtcgcatcga tcgaaccagc
      661 ttcgatgcct ggcgactgga gcagcgcgac cgcgacctgg atgcggagga ggacgactga
      721 attcgcagcg cggttttgcg cggtgtacac cgcgcaacgg gccacaaacg gggcaaaacc
      781 gcgcccaggt ttgcatgact tgcaatccgc aaaccgcgtt ggtaacgtcg tttacgcgtt
      841 tcaagcagag ggccagaggc gttcccaagc tggacgtcga gggttcgaat cccttcgccc
      901 gcttttgacg taaagtacgc tgaccccgct agttgcgggg acccgccttc gaggcggagc
      961 ggccgtaacc gaggccaaga cgcgtgtagt gtacacgcgg cgtttcggga gacgaccgtg
     1021 aacgcttcat caccgcagag actgcccaag ttccgggccc acaaggcgac actccaggcc
     1081 tacgtggagt tgtcgggcaa acgcttctac ctcggctgcc acgagtcggc cgaggcccgg
     1141 cagaagtaca acaccctcgt ggccgagtgg ctggccaacg gacgccagct ccgcgccaac
     1201 ccggagcaga tcactgacaa ggagctcacc acccgctact gggtctacgg cgcgcagcac
     1261 taccgccgcg ccgacggcac ggtcgcccgc gagctcgaca acatcgtgga cgccctccgc
     1321 ccggcgaagg aactgtacgg cacgaagccg actgcacagc tcggcccccg ggccctgagg
     1381 accatccgcc agaagatgat ccagagcggc cgtcgccgcc ggaatatcaa ctgccgcatc
     1441 gggcggatca agtgcgtgtt caagtgggcg gccggcgacg aattgatccc cggcgaggtg
     1501 taccacgccc tgccggcggt ggaaagcctg cgcggcggcc aaagccaggc aatagaaaac
     1561 gggtcctgaa tccaacacca accgaaccac gaaacgaatc catgccgacc ttcatggcca
     1621 tgccgacgct cccacaatat catcaacacc gaagactcac tgccccaccg gcttattcac
     1681 ggccagaacg ccgcgatggc cacaaccagc cagatcaaca ccccggccgt cagcttgacc
     1741 accgtgccga caaaacgacc gacacctgca ccaatgcccg ccttgagggc tgccaactgt
     1801 cggcgccccc ccgacaactc cagaccccag gccccaagcc cagcccccac acaagctccc
     1861 agaagcgagc ccaatagcgg aattgggatc aggaacgtcc cgaccagtgc cccaatcaaa
     1921 ccgccggcaa gtgccccgat cgagcctcgt cgaccagcac cagtggcttt ggcccccaac
     1981 accccagcaa ccagttccag cacctccgcc agagccgcca gaaccatgat cgcggccagc
     2041 accccgatac cgatcatcgg cgggtcaacc gtcgagtcgg ccggccactt ccaccacgca
     2101 accaacgcgg tcgtgatcac catcagccac gtgcccggca atcccagcgc caccaggatc
     2161 aaccaagccg cattcagcaa caacagcacg acgaccagca accagaacat cttcaccacc
     2221 cgggcgaatt cgggcgacac catactgaat ccgcatggcc caggccccgt ccctgctcga
     2281 cacactccgt cgtttccgga gcggccatct tgttattccc gcgatcaacc acaggtgaca
     2341 gcctgttcag cccggtcttg gctcccgagc ctgcaccccg gtgccgacag cgtctccccc
     2401 gctcattgac aattgcataa taccctcaac gccggccaga aaccggtgcc ctgtcgatcc
     2461 cacggcccct gtctgccccg gttgcctctt cttgctccat gcgaaacgaa cccatcgcag
     2521 ggggatccgc agaaacccag gcaaggcaca acaggccgca caactcgttc tgtaacaata
     2581 ctttacaacg acatcgcgaa aatcatccaa accccgggcg acccgacaga cctcttcgag
     2641 acggcccacg aatatgtgta tggcccgggt tatgttgacg aattcgtgtt ggagagccag
     2701 aagagcggca gcgcccagca ggacttctac atcctgcaag atgccaattt caacgtgatg
     2761 gccctgaccg acgccggcgg cggtgtggtc gagcagtatc aatgggagcc atacggtctc
     2821 ctcgcggcca aagacaccga caccggcggc accatccccc acagccacat cgggcatcag
     2881 ggactgttct tctaccggtt caatccccaa acaggcgaca ccgccaccct cagcacgacg
     2941 gccgtcgg
//
LOCUS       DAOTPW010000397         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_26340_length_2942_cov_5.404572, whole genome shotgun sequence.
ACCESSION   DAOTPW010000397 DAOTPW010000000
VERSION     DAOTPW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26340_length_2942_cov_5.404572"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..>2942
                     /locus_tag="P5316_21440"
     CDS             424..>2942
                     /locus_tag="P5316_21440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87578.1"
                     /translation="MKPFSRIRIVAAVVVAAAMLCTVKMAIGDEQGTAQWRLAFEDSF
                     DRPQPGEQWDFVLGQGKIVDGRMHVAANSDCVVAMINRDFADDVRLEFDAETDPKMPP
                     CDIACGLGGSNFINYGYLLQFGARGNQANQLYTSIARCMPYQLDEKPPFVIEYGKTYK
                     CVAEKEGRRVSYTVNGTKLLEVTDPDVLGGPGFRRVCLVTWSGMYVDHVKVYERVSPA
                     PDGPILIRKPESLDFGFRWENRTLAYSGGKSLPEQVRSGIEAYNARQYARAFELLTSV
                     KPPTRESVVASAYVLGDLAFFEKEADQQKVWELAQELAKTHPDNPATSDFALLAKWFS
                     GVTIPSRNKRDAERIFQIGPENNPFYYKAMLYRARYHLAWARESGLQRQSQEALELFL
                     ALKKLWPDHPGIADLTGEQTAWGPELIRAESEGPLWARLLQEGLARSHAILNWWFTER
                     QATDGQLGGGWGDDVELLRSWGMPACITSAQQIPVEGIRKLADGVWEHVLHGNGYPED
                     GDVEHAAEPAADSQPLMMLLRYGNPQYVERNLKTARFLRDVAMGVNQRGFLQFKSCYF
                     GTDGVNLHPSAVGQNIYHSRPMRHFVWLAWHGIPQARDVLLVWADGLREIAMAEIGPK
                     PAGYMPFTMFWPSGNIYPPENRFWLDERVHAYGMSPGKLWKYHFPLCSAYYFSRDRKF
                     LEPIDRMMDLASMSSGRAEYDQSLPRDHLKNLLADLSREPSVEVTTLYRWLTGSSIYD
                     EFGLSESMRLQTDRNIDRFAKALEPAISDLRINWAAKTSEVLQTDRFDIPGDFLIFSA
                     YTGAVTSLIEDGLIPTIAVTWDTPDLNFAALVLHASPDRMRV"
BASE COUNT          602 a          897 c          896 g          547 t
ORIGIN      
        1 agatgcaatc attgtcgcaa gctgattccg ataaagggcg cctccgcagg gcagaccgga
       61 gattcgaaca cgtgcccttg gtgcaagtcc gagcacagag aacattagcg gatagtgctc
      121 gccgcacgtc gaaatccagg tcaatcgggg tagcgggtga ggcacgccgg caagagcacc
      181 catggcaggg gttgggcaga ccccactgaa caggccgagg ctggtcgtga gtctcggggt
      241 accgcggttt gggaacacgc cgcatgcatt cgtgctccgt cctccgatcc ggagcaacgc
      301 ggcggtaaaa ctaaacggcg agagcacagt cggatggtaa cgtctaccgc tcagctcccg
      361 atgttcggca gcgagggaag cgatggttga cgtgagcaag agctcaatct gagagcagtg
      421 tgcatgaagc ctttctctcg aatcagaatc gttgccgctg tggtcgtggc agccgcgatg
      481 ctctgcacgg taaaaatggc cattggcgac gaacagggca ccgcgcagtg gcgactcgca
      541 tttgaagact cgttcgatcg gcctcaaccc ggcgagcagt gggacttcgt cctgggccaa
      601 ggcaagatcg tcgacggccg gatgcacgtg gccgcgaata gcgattgcgt cgtggccatg
      661 ataaaccgcg acttcgccga cgacgtgcgg ctggagttcg acgccgagac ggacccgaaa
      721 atgccgccct gcgacatcgc ctgcggtctg ggtggcagca acttcatcaa ttacggctac
      781 ctcctgcaat tcggcgcccg tggcaaccag gccaatcagt tgtacacgtc gatcgcccgt
      841 tgcatgccat accaactcga tgaaaagccg ccattcgtca tcgaatatgg caagacctac
      901 aagtgcgtcg ccgagaaaga gggtcggcgg gtcagctaca cagtcaacgg aacgaaactc
      961 ctggaagtca cggacccgga cgtgctcggc ggtccaggtt ttcgccgcgt ctgcctggtg
     1021 acgtggagcg gtatgtatgt cgaccacgtc aaggtctacg aacgagtctc gccggcaccc
     1081 gacggcccca tcctgatccg caagccggaa tccctcgatt tcggttttcg ctgggagaac
     1141 cgcacgcttg cctattcggg aggcaagtcg ctgcccgaac aagtccggtc cgggatcgaa
     1201 gcctacaacg cgaggcagta cgcccgagca ttcgagctgc tgacttccgt gaagccgccc
     1261 acgcgcgaga gcgtggtcgc atcggcttat gttctcggcg acttggcgtt cttcgagaaa
     1321 gaggcggatc agcagaaggt atgggagctt gcgcaggaac tggcgaaaac tcatccggac
     1381 aatccggcga cctctgattt cgccctgctg gccaagtggt tcagcggtgt gaccattccg
     1441 agtcgcaaca aacgagatgc cgagcggatc ttccagatcg gccccgagaa caaccccttc
     1501 tactacaagg caatgctgta ccgcgcacgg taccacctgg cttgggcccg cgagtcaggc
     1561 cttcagcggc agtctcagga agcactggag ctttttctcg ccctcaagaa gctctggccc
     1621 gaccacccgg gaattgccga cctgaccggt gaacagacgg cctgggggcc ggagctgatt
     1681 cgggctgagt ccgaggggcc gttgtgggct cgactcttgc aggagggact ggctcgctcg
     1741 cacgccattc tcaactggtg gtttactgag cggcaggcga ccgacgggca actgggcggc
     1801 ggctggggag atgacgttga attgctgcga tcgtggggaa tgcccgcgtg catcaccagc
     1861 gctcagcaga tacccgtcga gggcatccgg aagctcgccg acggcgtctg ggaacatgtg
     1921 ctgcacggaa acggataccc cgaagacggt gacgtggagc acgcggcgga gccggccgct
     1981 gattcgcagc ccttgatgat gctccttcgc tacggcaacc cccagtacgt cgagcggaac
     2041 ctgaaaacgg ccaggtttct ccgcgacgtc gcaatgggag tcaaccagcg cggcttcctg
     2101 cagttcaagt cgtgctattt cggaaccgac ggcgtcaacc tgcatccgag cgccgtcgga
     2161 cagaacatct accattcgcg gcccatgcga cacttcgtct ggctggcttg gcacggaatt
     2221 ccgcaagccc gcgacgtgtt actggtctgg gcggacggcc tccgcgagat cgccatggcc
     2281 gagatcggcc caaaacctgc cggctacatg cccttcacca tgttctggcc ctcgggcaac
     2341 atctatccgc ccgagaaccg tttttggctg gatgagaggg tccacgccta tggtatgtcg
     2401 ccaggcaagc tctggaagta ccatttcccc ctttgcagcg cctactactt cagccgcgat
     2461 cgcaagttcc tcgaaccgat cgaccggatg atggacctgg caagcatgtc ttcgggtcgc
     2521 gcggagtatg accagagcct gcctcgcgat caccttaaga acctgttggc cgatctgtcc
     2581 cgtgagccgt cggtcgaggt gaccacgctc taccgctggt tgacgggctc atcaatctac
     2641 gacgaattcg gcctgtccga atcgatgcgt ctgcaaacgg acaggaacat cgaccgtttt
     2701 gccaaggccc ttgagcccgc gatatcagac ctgcggatca actgggcggc aaagacaagc
     2761 gaggtccttc agacggaccg ttttgacatt cccggcgact tcttgatctt ctcggcgtac
     2821 acgggcgccg taacctcgct catcgaagac ggcctgattc cgacgatcgc cgtcacatgg
     2881 gacacgcctg atctgaactt cgccgcactg gtgttacacg cttcacccga ccgcatgcgc
     2941 gt
//
LOCUS       DAOTPW010000398         2882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_27022_length_2882_cov_4.391227, whole genome shotgun sequence.
ACCESSION   DAOTPW010000398 DAOTPW010000000
VERSION     DAOTPW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27022_length_2882_cov_4.391227"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="P5316_21445"
     CDS             complement(<1..1049)
                     /locus_tag="P5316_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRR87579.1"
                     /translation="MHPSRPTILCAMISLAAACPTFGVRPATWLHEQPKDFNEGKLDG
                     TVVSSLGEVTLGRQTRVLHELKEQGQVVNAIARAGDGRIYLATGPKGIIYRVDGEKVT
                     EFATLPEGGTVLSLLFTKDGNLLAGTGGGPQAKIYLIDGNGRSRVFHEPPKAVYIWAM
                     VRGVEGEVYAATGNKGQIFKIEPDGSKSKVLCDLKPKNVLCMAFAPDGFIYAGTDEDG
                     LVYRVDPAGGQVFVVYDAKEPEISAIVFDAQGNLYASTADAEGARPGRTVADKPGGRP
                     ETQRAEGSTGKGILSLLKTGTPRTASQPAAGSSTAPASQPESRAGTSDDDEDAGDDGS
                     TAGPATQQAGLLSRVG"
     gene            complement(1127..>2882)
                     /locus_tag="P5316_21450"
     CDS             complement(1127..>2882)
                     /locus_tag="P5316_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87580.1"
                     /translation="FGVEVISVMHNAFYAKQDVILIRCSGLNLEHSGVIGGMSGSPCY
                     IRDEQGRERLIGAVAYGWTFSKDPICGVQPIAQMLDVANVRKPKPETRPSGETQPVVQ
                     AWGSPPGGGFPLQDWILKAVGEPIPADCRLSVLSNPSPSPLPDKTAATLLPEGLRPLE
                     IPLSMSSLSQESMTLLGGWFKRHGLTPLAAGGAPGAGQAPPGKVQLEPGAALCVALVT
                     GDIFIDAVGTCTAVIGDRVLGFGHQFMGTGPVEYPLATGFIHTVIPSVMRSNKLGGAL
                     EVVGTLWGDEATAVFGTVGRLPAMVPMEVVVNDLRGRQTFHYNVVHEDESTGAFLATC
                     LMESIYAHNELPIDHTVRHNVEVDFGELGTYTSSNVSSQSGVREAAVDLMYPVQTLLN
                     PPLAQKARVKKVRAEITIEDKAQRATIYHASLPKTVYKPGETVTAAVRWSHYRKDPLY
                     TDGWYSLKLPDDLPDGDYEVVLCSNSGHLTSLNSEKPHLFRAETLSQAMAAFNLMGRL
                     PGNVLYMRLNLKQGGIAYKQLEMPDLPASRRKILSDTKLASDLNPYTEALVAEHKTDF
                     VVLGKQALPIRVRRHPDS"
BASE COUNT          541 a          853 c          914 g          574 t
ORIGIN      
        1 cccacccgcg aaaggaggcc ggcctgttgg gtggcggggc cggcggtaga accatcgtcc
       61 ccggcgtcct catcatcatc ggacgtaccg gcccggcttt caggctgcga ggccggcgcg
      121 gtgctggaac ctgcggccgg ctgcgaggcc gtccgaggcg tgccggtttt aagaagcgaa
      181 agaatacctt taccagttga gccctcggct ctctgggtct caggcctgcc gccgggctta
      241 tcggcgacgg tacggcccgg ccgagcgcct tcggcatcgg cggtggaagc gtacaggttg
      301 ccctgggcat cgaagacgat agcgctgatt tcgggctcct tcgcatcgta aacgacgaag
      361 acctgtccgc cggcggggtc gacccggtac accaacccgt cctcatccgt cccggcgtaa
      421 atgaacccat ccggcgcaaa cgccatgcag agaacgtttt tcggcttgag gtcgcacaga
      481 accttgctct tggagccgtc gggctcgatt ttgaaaatct gccctttgtt tccggtggcc
      541 gcataaacct caccttcgac gcctcggacc atcgcccaga tgtataccgc cttagggggc
      601 tcatgaaaga cgcggctcct gccgttgccg tcgatgagat agatcttcgc ctgcgggccg
      661 cctccggtgc cggccaggag attgccatcc ttggtgaaca ggagggacag cacggtgccg
      721 ccctccggaa gggtcgcgaa ctccgtcacc ttctcgccgt ccacgcgata gatgattccc
      781 ttcggccctg tcgcaaggta gatcctgccg tcacctgctc tggcgatggc attgaccacc
      841 tggccctgct ccttcaattc atgaaggacc ctagtctgcc ggccgagtgt cacttcgcca
      901 agagatgaga cgaccgtgcc gtcgagcttg ccctcgttga aatccttggg ctgttcgtgc
      961 aaccaggtgg cagggcgcac gccgaaggtc gggcaggcgg ccgccaggct gatcatcgca
     1021 cacaaaatgg tcggacgtga tgggtgcata attcacactt tcttaaagta ggacgcagca
     1081 gctcgagcta cctctgattc atcccggcag gccggcgtct atgacactat gaatcgggat
     1141 gccttctcac ccggatcggc aaagcctgct tgccaaggac gacgaaatcg gtcttgtgct
     1201 ccgcgaccag ggcttcggtg taggggttca ggtcggaagc aagcttggta tctgacagaa
     1261 tcttgcggcg tgacgccggc aagtcgggca tttccaactg cttgtaggcg atgccccctt
     1321 gcttgagatt gagacgcatg tacagaacgt tgcccggcaa gcgtcccatc aggttgaacg
     1381 cagccatggc ctgggacagc gtttcggccc tgaaaagatg cggtttctcg gagttgaggc
     1441 tcgtcaagtg accggagttg gagcagagta ccacttcgta gtcgccgtcg ggaaggtcgt
     1501 ccggcagctt gagagagtac catccgtcgg tgtagagggg atccttgcgg taatggctcc
     1561 agcgcacggc cgccgtgacg gtctcgcccg gtttgtaaac ggtcttgggc agggaggcat
     1621 gatagatggt tgctcgttgt gccttgtcct cgatggtgat ctcggcgcgg acctttttga
     1681 cacgggcctt ttgggcaagg ggcgggttga gcaacgtctg gaccggatac atcaggtcga
     1741 cggccgcctc gcgcactccg ctttgcgagc tgacgtttga cgacgtgtac gtacccagct
     1801 cgccgaaatc aacctcgacg ttgtgtcgga ccgtatggtc gatcgggagc tcgttgtgcg
     1861 catagatgct ttccatgagg caggtagcga ggaaggcccc ggtggactca tcctcgtgaa
     1921 cgacgttgta gtgaaaggtc tggcgacctc gcaggtcatt gaccacaacc tccattggga
     1981 ccatggcggg caacctgccg acggtgccga atacggccgt ggcctcgtct ccccacaagg
     2041 ttcccacgac ttcaagtgct ccgcccagct tgttggatcg catgacagag gggataacgg
     2101 tatgaataaa gccggtggcc aaggggtatt cgacaggtcc tgtgcccatg aactgatgcc
     2161 cgaagccaag cacccggtcg ccaatcacgg ccgtgcaggt tcccacggcg tcgatgaaaa
     2221 tgtctccggt cacgagtgcc acgcagaggg cggcccctgg ttcgagttgg acctttccgg
     2281 gaggagcttg gccggcaccc ggcgctccgc cggcagcaag cggcgtcaag ccgtgccgct
     2341 taaaccagcc gccgagaagc gtcatcgact cttgactgag cgaggacatt gagaggggga
     2401 tttcgagggg tcgaagcccc tcgggcagca gtgtggcagc ggttttgtcc ggcaggggcg
     2461 acggcgaggg attgctgagg acactgagtc ggcaatcggc ggggatgggc tcgccaacgg
     2521 ccttcagaat ccagtcctgc aacgggaagc caccgcctgg gggcgatccc caagcctgca
     2581 caacgggttg ggtctcgccg gatggccggg tttcgggttt cggctttcgg acgtttgcca
     2641 cgtcaagcat ttgtgcaatc ggctgaacgc cgcagattgg atccttgctg aacgtccaac
     2701 cataggcaac tgcaccgatg agacgttccc gaccctgctc atcacgaatg tagcagggcg
     2761 aaccgctcat gccgccgatg acgccgctgt gttcgagatt gagcccggag caccggatga
     2821 gaataacgtc ctgtttcgcg tagaaggcgt tgtgcattac cgaaataact tcgacgccga
     2881 ag
//
LOCUS       DAOTPW010000399         2794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_28165_length_2794_cov_6.439211, whole genome shotgun sequence.
ACCESSION   DAOTPW010000399 DAOTPW010000000
VERSION     DAOTPW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28165_length_2794_cov_6.439211"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="P5316_21455"
     CDS             <1..664
                     /locus_tag="P5316_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87581.1"
                     /translation="TERWRTAVTGRLSQIFKPAADLAAIADRAGQVEVRESSTGKPRG
                     RFATPTCAEGVIDGALVDGVFYVIGFFERPARRPRRVNGCYGLGAIRVEDGTILWQRN
                     DIAPGTFLTADVLRMSSNAIPLAMLVSDSEASGTSRPAGTRARSSTGDPPGKIEMCLL
                     DKATGNEIGRRVSKLLPTGSRGSLILDVCVWGREIIVRTAGCEVRFAVETHTHHSAPW
                     VR"
     gene            678..1721
                     /locus_tag="P5316_21460"
     CDS             678..1721
                     /locus_tag="P5316_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRR87582.1"
                     /translation="MIILVLVSVFTIADGLTARAELPPALPPLVTPETQSAIDRGLEY
                     LARTQSRDGAWRHSGSWGHYPTAMTALAGLTMLANGNTATQGKYAPNVSRAASFLLAS
                     AQPSGLIAREEESGRSMHGHGFAMLFLGQLHGMEEDNEKQERIASVLRRAVELTARSQ
                     SRQGGWLYSPDSSGDEGSVTVTQLQGLRASRNVGITVPKKVIDGAMKYLDLSTLPDGG
                     ISYRADRQGGSRPPITAAAVACWYNAGLYDYPAAVKALEYCKLRVGNGQARTTGIWGH
                     YYYAHLYMSQIMWLSSKENWEWYFPTMRDWLLAQQASDGSWEGDGVGKVYGTAIATFI
                     LQIPYGYLPILQR"
     gene            1880..>2794
                     /locus_tag="P5316_21465"
     CDS             1880..>2794
                     /locus_tag="P5316_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR87583.1"
                     /translation="MNESLSSPVQDDLSAINRLREAHERICEGLSRVIVGQKDVVEQL
                     LIALFAGGHCIIEGVPGLAKTLMISTLARSLSLSFSRIQFTPDLMPSDITGTEVIQED
                     RATGTRSFRFLRGPVFANVVLADEINRTPPKTQAALLEAMQERQVTAGGKRHPLEPPF
                     FVLATQNPIEQEGTYPLPEAQQDRFMFKVFVGYPSYEEELRIAELTLGDVAAAVESIQ
                     PALGAEEVLDLQKTVQRVPAARRVVAHALDLVRASRPDEPSAPEVVRRMIRWGAGPRA
                     VQFMVAAAKARAILHGQYHVSAEDIRAVA"
BASE COUNT          523 a          794 c          960 g          517 t
ORIGIN      
        1 caccgagcgg tggcgaacag ccgtgacggg gcgactgtcg cagatcttca agccggccgc
       61 cgatctggcg gcgatcgccg accgcgcggg tcaggttgag gtcagggaat cgtcgacggg
      121 caaaccccga ggccggttcg cgacgccgac ctgtgcggag ggggtgatcg acggggctct
      181 ggtggatggc gtgttctacg tcatcggttt tttcgaacga cccgcccgaa ggccccgccg
      241 agtgaacggc tgttacggtc ttggggccat tcgtgtggag gacggtacaa ttctatggca
      301 gcggaacgat atcgctcccg gtacgttctt gacggcggat gtgctgcgga tgtcatcgaa
      361 tgcgatcccg ctggctatgc tggtttcgga ttccgaagcc agcggcacga gccggccggc
      421 agggactcgc gcgcggtcgt cgaccggtga cccgccaggc aagatcgaga tgtgtttgct
      481 ggacaaggcc acagggaacg agatcggacg gcgggtcagc aagctgctgc cgacgggctc
      541 gcgcggaagc ctgatcctgg acgtttgcgt atggggcagg gagattatcg ttcgcacagc
      601 cggttgtgag gtacgatttg ccgttgagac tcatacccat cattcggcgc catgggtgag
      661 ataagatgag ccgtcgcgtg atcatactgg ttcttgtatc ggtgtttacc atcgccgacg
      721 ggctcacggc acgagccgaa ctgccgccag ctcttcctcc gctggtcacg ccggagacgc
      781 aaagcgcgat cgaccgcggg cttgagtatc tggcccgcac gcaaagccgc gacggcgcct
      841 ggcggcacag cggatcatgg gggcactacc cgacggcgat gacggctctg gccgggctga
      901 caatgctggc caacgggaac accgcgacgc aaggcaagta tgctccgaat gtcagtcggg
      961 cggcgagttt tctgcttgcc tcggctcagc caagcggcct gatcgcacgt gaggaagaat
     1021 cggggcggtc aatgcacggt cacggttttg cgatgctctt tctgggccag cttcacggca
     1081 tggaagagga caacgagaaa caggaacgta tcgcctcggt tcttcgccga gccgtcgaac
     1141 tgacggcgag aagtcagagc aggcaaggag gctggctcta ttcgccggac tcttccggcg
     1201 acgaaggctc ggtgacggtc acgcaacttc aggggctgcg ggccagtcgc aacgtcggca
     1261 tcacggtacc taagaaggtg atcgacggtg ccatgaaata cctcgacctg tcgacgctgc
     1321 cggatggggg gatttcctat cgggccgatc gacagggggg cagccggccg ccgattactg
     1381 ctgcggcggt ggcgtgctgg tacaacgcgg gcctctatga ttacccagcg gcggtcaagg
     1441 ccctggaata ctgcaagctg cgtgtcggca acggccaggc gcgcaccacg ggcatctggg
     1501 gacactacta ttatgcccac ctgtacatgt cccagatcat gtggctcagc agcaaagaga
     1561 attgggagtg gtattttccg acgatgcgag actggctgtt ggcgcagcag gcgtctgatg
     1621 gttcgtggga aggcgacggc gtcgggaagg tgtatgggac ggcaatagcg acgttcatcc
     1681 tgcagattcc gtacggctat ttgccgattc tgcagcgatg aacccgtgac ggtgcagtcc
     1741 gaaggccggt gcgggatcag catcttgccg gtccagtgac aggcccttga ccagcccccg
     1801 taagtcggcc aagggcaggc tggaggcctg ttccaccctg ccgcaggttg cgttgtgaca
     1861 cagaacggaa gcattggtca tgaatgaatc attatcgtca ccggtccagg atgatctgtc
     1921 ggcaatcaat cgtctgcggg aagctcacga gcgcatctgc gaaggtttat cgagagtgat
     1981 cgtcgggcag aaggacgtgg tcgagcaact gctcatcgct ctgtttgccg gcggtcactg
     2041 catcatcgag ggcgtgccgg ggctggccaa gacgctgatg atttcgacgc tggcccgatc
     2101 gctgagtctg tccttttcgc gaatccaatt cacgcccgac ctgatgccgt cggacatcac
     2161 cggcaccgag gtgattcagg aggatcgggc gaccggcacg cgatcgttca ggttcttgcg
     2221 cggaccggtg ttcgcgaacg tcgtactggc cgacgagatc aaccggacgc cgccaaagac
     2281 tcaagcggcg ttgctggagg cgatgcaaga gcggcaagta accgcggggg gcaagcggca
     2341 cccgctcgaa ccgccgttct tcgtgctggc cactcagaac ccgatcgagc aggaaggcac
     2401 ctacccgcta ccggaggctc agcaggaccg cttcatgttc aaggtgttcg tcgggtatcc
     2461 cagctatgag gaggaactgc gcattgcaga gctgacgctc ggagacgtag cggcggcggt
     2521 ggaatcgatc cagccggctt tgggggctga ggaagtgctt gacctgcaga agacggtcca
     2581 gcgtgttccg gcggccagac gggttgtcgc gcatgctctg gacctggttc gggccagccg
     2641 tccggacgag ccttcggcac cggaggttgt gcggcggatg attcggtggg gtgccgggcc
     2701 gcgagcggtg cagttcatgg tcgccgctgc aaaagccagg gcgattcttc acggccagta
     2761 ccacgtgtcc gccgaggata tccgggccgt cgcc
//
LOCUS       DAOTPW010000400         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_28252_length_2787_cov_6.530381, whole genome shotgun sequence.
ACCESSION   DAOTPW010000400 DAOTPW010000000
VERSION     DAOTPW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28252_length_2787_cov_6.530381"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            763..1161
                     /locus_tag="P5316_21470"
     CDS             763..1161
                     /locus_tag="P5316_21470"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRR87584.1"
                     /translation="MKYLVVWSIALVLCLGLTAGCKQEKEMAAAPEEQAEPPMFPEET
                     VPKVEPAAAPAPVDVTAAAPAPKPAPKESYAEPKAPRTYVIQKGDTLQKISEKFYGTT
                     KKFDKIYQANKDVLKDKNTLKVGTKIKIPD"
     gene            1224..>2787
                     /locus_tag="P5316_21475"
     CDS             1224..>2787
                     /locus_tag="P5316_21475"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRR87585.1"
                     /translation="MGPAAADHASRQFRPATPHSRIPASPDAQSPLDKPVQFVRGVGP
                     HRARLLSKIGINTVGDLLEYFPFRHEHQRRPQSIDSLVLDANATVIGIVDSVRSNLTR
                     HGGPSVTLRLSDGTGTVRVAWFNAPYLRDRFHRGQVLRIHGQVGEFEALAQFVNPTVE
                     QLDPQADPKTWDFERVVPIYKATASLSTTHIARIVDNALKETLNSIDETLPETLRKRR
                     QLIGRREAIWRMHRPDNPEQVIEARRRLAYDELFAMQLAIALQRRWTATGAKAPRLPV
                     TPEIDQRIRRRFPFPLTAAQNRVIAEICADLARDRPMTRLLQGDVGSGKTVVALYAAL
                     TAIANKTQCAILAPTEVLAAQHFAGIERYLAGSRVERCLLTGNTPRSQRDRLLESVAK
                     GTTNLVVGTQALLEKTVEFADLGLVIVDEQHKFGVSQRAIMRSKKTRSRHGAASLLPH
                     YLVMTATPIPRTLSMTVFGDLDISTIDSSPPGRQPIHTKIVRPKEEAEAWLAVRHRIR
                     EGDQAYVVYPLVE"
BASE COUNT          614 a          883 c          809 g          481 t
ORIGIN      
        1 tgggcatgcg cctccaaatg aatcctgaag caggtagggc aggtcagagc cctgcaaagt
       61 cgcatcgacg aagcagggcg ccgacctgcc gaaccacgga taggggtagg aagggcggat
      121 tgaccgtccc ccctccctcc gaaccggatc gctcggcccg caatcctttc cgggccgcat
      181 cccctgcgga atcctttccg cattcggcat ccatgacgat gccaatcctc cacccgaagg
      241 ccagcaggta gggcaggtca cagccctgcg aagtcgcatc gacgaagcag ggcgtcgacc
      301 tgccgccacc ctgaccgccc cccgcgccaa acgaacccac ccagccagca tgcagcaatt
      361 ataagtattt gtgaaataag tacgtaaggc atgatcgtct gctcttccat gccgtggcgt
      421 gctggtgacc gcactggccc acgatagcca acgggctggc tgcggcacct ccaagccttt
      481 ccggtgataa cgctcttcat ttcccgctca gaaccgttgt gtgttgaatg ggcgtcgacc
      541 ccagacgaaa tacagtcagc accggggagc tgcgatccca atgccctcgt cttggtccga
      601 gtatcctcca tcgcacccct gaggcgagga aaaacgcata cagtcgtttt gtggacgaga
      661 tattagaggg cgccgaattc attgataggc ccttattgga cgatatcatt cacgcgccga
      721 tttggcgtgg atgaagtgga agtctattta aggagaacga cgatgaaata cctggttgtg
      781 tggtcaattg ccctcgtgct gtgcctgggt ctgacagccg gctgcaagca ggaaaaggaa
      841 atggctgcag ctccggagga acaggccgag cccccgatgt tcccggagga gacggtgcca
      901 aaggtcgaac cggcggccgc accggccccc gtcgatgtga ctgcggcggc gccggcaccc
      961 aagcctgctc ccaaggagag ttacgccgag ccgaaagccc caagaaccta cgtgattcag
     1021 aagggcgata cgctccagaa gatctccgag aagttctacg gcacaacgaa aaagtttgac
     1081 aagatctacc aagcaaacaa ggatgtgctc aaggacaaga acaccctcaa ggtcggcacc
     1141 aagatcaaga tcccggattg atctgatccg ggtgatcgac cggatggcag gaatgcctgt
     1201 acccctggag ggaggctgca agagtgggac cggcggcggc ggatcatgca tcgaggcagt
     1261 tccgccccgc cactccgcac tccaggattc ctgcgtcgcc tgatgcccaa tcgccgctgg
     1321 acaagccggt gcagttcgtg cggggtgttg gtccacacag ggcaagactc ctgtccaaga
     1381 tcgggatcaa caccgttggg gacctgctcg agtatttccc ctttcgccac gagcaccagc
     1441 gtcggcctca atccatcgac agcttggttc ttgacgccaa tgcgacggtt atcggcatcg
     1501 ttgactcggt tcggagcaac ctcacccgcc acggcggccc aagcgttact ctgagactga
     1561 gcgacgggac cgggaccgtc cgggtcgctt ggttcaacgc cccctacctg cgagaccgct
     1621 tccaccgggg gcaggtgcta cggattcacg ggcaggtcgg cgaattcgag gcccttgccc
     1681 agtttgtcaa cccgaccgtc gagcagctcg atccccaggc cgatcccaag acgtgggatt
     1741 tcgagcgggt ggtgccgatc tacaaggcca ccgcctcgct atcgaccacc cacatagctc
     1801 gaatcgtcga caacgccctc aaagaaacgc tcaattccat cgacgagacc ctgccggaaa
     1861 ctctgcgcaa acgtcggcaa ctgatcggtc gtcgcgaagc catttggcgc atgcatcggc
     1921 cggacaatcc ggaacaggta atcgaagccc gacgacgctt ggcctacgac gagctgttcg
     1981 ccatgcaatt ggcgattgcc ctgcagcgcc gatggacggc caccggagcg aaggcgccaa
     2041 gactgccggt gacccccgag atcgaccaaa gaatccgccg ccgcttcccg tttccgctga
     2101 ccgccgcaca aaacagagtc atcgccgaga tctgcgccga tctggcacgc gacagaccaa
     2161 tgacccggct gctgcaaggc gacgtcggta gcggaaaaac cgtggtcgcc ttgtacgcgg
     2221 ccctgacggc catcgccaac aagacgcaat gtgccatact ggcgccgacg gaggtcctgg
     2281 ccgcacagca ctttgccgga atcgagcgat acctggccgg cagtcgcgtc gaaaggtgcc
     2341 tcctgaccgg caacacgccc agatctcaac gagacaggct cctggaatcc gttgccaagg
     2401 gaacgacaaa cctcgtcgtc ggaacccagg cattgctgga aaaaactgtt gagtttgcgg
     2461 atctcggcct ggtgatcgtt gacgagcagc acaaattcgg cgtttctcag cgggccataa
     2521 tgcgaagcaa gaaaacgaga agccgccatg gtgctgcctc gctgcttccg cactatctcg
     2581 tcatgaccgc aacgccgatt ccgcggacgc tgtcgatgac cgtcttcggc gacctggaca
     2641 tctccacgat cgactcgtcg ccccccggac gtcagccgat ccacacgaag atcgtgcgtc
     2701 cgaaggagga agcggaagca tggctggcgg tccgacatcg gatccgcgag ggcgatcagg
     2761 cctatgtggt ttacccgctc gttgagg
//
LOCUS       DAOTPW010000401         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_28586_length_2761_cov_4.691426, whole genome shotgun sequence.
ACCESSION   DAOTPW010000401 DAOTPW010000000
VERSION     DAOTPW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28586_length_2761_cov_4.691426"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="P5316_21480"
     CDS             <1..1349
                     /locus_tag="P5316_21480"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRR87586.1"
                     /translation="GTTFDPAPEITAIELWFSEPISYTAGDFSVTGSVSGPQEFSVNW
                     DTAAYKITLSFAAPLAADQVWTVQVAATLTSSAGAKALDGELDAGFPSGDGVPGGNAV
                     FSFTLGQSPPPCSDPFADADGDGDVDQADFGAFQRCLTGDGDPQETFDGVGCRCFDHD
                     DDWDVDEIELSLMLACMSGPGIMADETCDDQWTLLADDFDAGASAGNWTVYASSADYT
                     ADFAFDYSTRGIPPAPNSTGGTTIGLHLTVNSNDSTPATAAVSLFPTGRRFSGNYTLA
                     FDMWMNYAGTGGGVGTTEMMNAGINTVDTQVIWPGNPGSGIFMAATGDGDDTIDYRCD
                     VGSTLLAPSSGAYAAGTHSTARDNTDPYYQSLFPSPPFETRGSPGKQWVRVEIAQNAG
                     TVTWKLNGTPIVNLTGAAYTAGNVMLGYMDLFSSISVPPEDTFIIYDNVTVTASAP"
     gene            complement(1763..2725)
                     /locus_tag="P5316_21485"
     CDS             complement(1763..2725)
                     /locus_tag="P5316_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRR87587.1"
                     /translation="MLDQGLVGLYVGRMTPGSPEGDTATLTAYDSDGYVMGQVQRHLP
                     DASQGITGFIGLGATYADQPIKTIELSFGGTAMKEPVRVDQLVMCYTKAELEFPTFPP
                     PPAFGEPVYTVLVQAVESQAKGPAVAGDPGHNSMSSAPLSGVIMKVDGEPQNTTFAFH
                     RHEGTQVSLLAPESFNGLRFVYWRSDDGTSFALGQTDILLPILREATLTAVFMADTDG
                     DGRMDEADNCPSNANPDQADDDNDGVGNECDLCPGFDDHVDADGNGMPDGCDAAIEPH
                     DGTGPRPAVCGMGLATMVPLVVAGLMVIGMVSRLKSGHISQDHT"
BASE COUNT          504 a          950 c          773 g          534 t
ORIGIN      
        1 cgggcacgac cttcgacccc gcccccgaga tcaccgccat tgaattgtgg ttcagcgagc
       61 ccatcagcta cacggccggc gatttctcgg tcaccggcag cgtgagcgga ccgcaggagt
      121 tcagcgtcaa ctgggatacg gcggcctaca agatcacgtt gtcgttcgca gcaccgctgg
      181 ccgccgatca ggtctggacc gtccaggttg ccgccacgct cacctcatcg gcgggtgcca
      241 aggccctcga tggcgaactc gatgccgggt tcccctcggg cgacggtgtg cccggcggca
      301 acgcggtgtt ctccttcacc ctcggccagt caccgccgcc ctgctcggat cccttcgctg
      361 acgccgacgg cgacggcgac gtggaccagg cggatttcgg agcttttcaa cgctgcctga
      421 ccggcgacgg cgatccccag gaaaccttcg acggcgtcgg ttgccggtgc ttcgatcacg
      481 atgacgactg ggacgttgac gagatcgagc tttcgctgat gctggcgtgc atgagcggcc
      541 cgggcatcat ggccgatgag acttgcgacg accagtggac gctgctggcc gacgatttcg
      601 acgccggcgc ctcggccgga aactggaccg tctatgcgtc gtcggccgac tacaccgccg
      661 acttcgcttt tgactactcg actcgcggga tcccgccggc ccccaactcg accggcggaa
      721 ccacgatcgg cctgcatttg accgtcaata gcaacgactc gacgccggca accgctgccg
      781 tcagcctttt ccccaccggc cgaaggttca gcggtaatta cacgctggct ttcgacatgt
      841 ggatgaatta cgccggcacc ggcggcggcg tcggcaccac cgagatgatg aacgccggaa
      901 tcaacacggt cgatacccag gtcatctggc ccggcaatcc tggcagcggc attttcatgg
      961 ccgcgaccgg cgacggcgac gacaccatcg attatcgctg cgacgtcggc tcgacgcttc
     1021 tggctccgtc atcgggcgct tacgccgcag gcacacacag caccgcccgg gacaacaccg
     1081 acccctatta tcagagcctg ttcccctcgc cgccgttcga gacacgagga tcgcccggca
     1141 agcagtgggt ccgagtcgag atcgcccaga acgccggcac cgttacctgg aagctgaacg
     1201 gaacgccgat cgtcaacctg accggcgcgg cctacaccgc cggcaacgtg atgctcggct
     1261 acatggacct gttctcgtcg atctccgttc cccccgagga caccttcatc atctacgaca
     1321 atgtcacggt cacagcctcc gccccgtgac cgccttcggg cgtcgctgtc tctccgtggc
     1381 tcgaattccg atcccaaacc ggttggcgtc tcgatgatgt atcccgaagc ccggtaggtc
     1441 gtgctccgca cgacatcctc ttgtcagacc aacggactaa cagaccaaca gaccaacgga
     1501 ctaatagacc aatagaccaa cagcccagta ggtcgtgctc cgcacgacgc cttcttgcca
     1561 gaccaacaga ctaacagact gatggactaa tagaccatta ttcattcttc gatcccggcc
     1621 catcctcctc gtttccatct tcgccctctg ccttctcccg tctcgcgccg ccaaactagc
     1681 tgcgtccccg tttcggcttc ccgttgcaga tcccgcgctt gacaagacgg gggctaatgg
     1741 gtgatccctt ttgcgtcccc tcttatgtat gatcctgact gatatgcccc gacttgaggc
     1801 ggctgaccat cccgatcacc atcagaccgg ccaccaccag cggcaccatg gtagctaacc
     1861 ccatcccgca cacggccggt cgtggtccgg tcccgtcgtg aggttcgatg gctgcatcgc
     1921 aaccgtccgg catgccgttg ccgtctgcgt cgacatggtc atcgaaaccc ggacacaggt
     1981 cgcattcatt gcccacaccg tcgttgtcgt cgtcagcctg atccggattg gcgttcgacg
     2041 gacagttgtc cgcctcatcc atgcggccgt cgccgtccgt gtcggccatg aacacggccg
     2101 tcaacgtcgc ctcacgcagg attggcagca agatgtccgt ctgccccagg gcgaatgaag
     2161 tcccgtcgtc ggatcgccaa tacacaaaac gcaatccgtt gaaactctcg ggcgcaagca
     2221 gcgagacttg ggttccctcg tgccgatgaa aagcaaaggt cgtgttctgc ggttcgccgt
     2281 ccaccttcat gattacaccg ctcagcggtg cggaggacat gctattgtgt cccggatctc
     2341 ccgcgaccgc cgggcctttg gcctggcttt cgaccgcctg caccaggacc gtatagaccg
     2401 gttcgccgaa agccggcggg ggcgggaagg tcggaaactc cagctccgcc tttgtgtaac
     2461 acatcacgag ttggtccacc cgcaccggtt ctttcatggc cgttccgccg aagctcaact
     2521 cgattgtttt gatgggctga tcggcgtaag tcgcgcccag accgatgaaa ccggtgattc
     2581 cctgcgacgc atcaggcaga tgccgttgta cctggcccat gacgtagccg tcactgtcgt
     2641 aggcggtcag agtggccgtg tcgccttcgg ggctgccggg cgtcatccgg ccgacgtaca
     2701 gacccactag cccctgatca agcatactcc ggcgaggttt tctcgctttg ttcgtaaaaa
     2761 a
//
LOCUS       DAOTPW010000402         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_28883_length_2741_cov_4.603872, whole genome shotgun sequence.
ACCESSION   DAOTPW010000402 DAOTPW010000000
VERSION     DAOTPW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28883_length_2741_cov_4.603872"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1391
                     /locus_tag="P5316_21490"
     CDS             315..1391
                     /locus_tag="P5316_21490"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HRR87588.1"
                     /translation="MFGTRKWLGVVLLAATIQTSYLAAQQTAPAGRGALRRELIERFD
                     KDGDGEISREERAAARKGLRQGGRVQQKLVETVLPESVRVVRDVEYARVGDQPLLLDL
                     YLPKPEGEPFPVVIWVHGGAWQAGSKDLCRAAFLSGEGYAVVSINYRLTDVAPFPAQI
                     HDCKAAVRWVRAHAKEYGFDPARIGVWGSSAGGHLVALLGTSAGIRELEGDIGGNLDQ
                     SSRVQAVCDFCGPSTFRAEDYEHEPDKPRGAGPQVVQKLLGGPLNKNPEKARLASPIE
                     HVSKDDPPFLIVHGEKDNVVPVKHSRLLADALRKAGADVTLKVVPDAGHGVGGLANRK
                     MVAEFFDQHLKRRPASRPVGSAAE"
     gene            1388..2233
                     /locus_tag="P5316_21495"
     CDS             1388..2233
                     /locus_tag="P5316_21495"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRR87589.1"
                     /translation="MMRRYRFLLMATAVATLLWVTGCDAGRGAGGSAVKPVRTLTVAA
                     VSCESRIHEVEFNLSRIEHWARQAAAAGADIALFPETGISGWWASREVRAFAEPIDGP
                     AITRLTKVADELGIVLAVGMTERDGDKAHITHVVIDGRGVIGMHRKTSLAPGEEKTWD
                     PGSDANVFEVKGIRVGIAICFESVHPPTCARLRENGAEIILAPYANGTDPDELLNGKR
                     PYPYERAKENQVWYVACDAPARDEQKNLRRGAAYVISPEGELKAITAPDAVGETMVLY
                     TIRLS"
     gene            complement(2385..>2741)
                     /locus_tag="P5316_21500"
     CDS             complement(2385..>2741)
                     /locus_tag="P5316_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87590.1"
                     /translation="DLAIDMPGCREITENGIQERNPLFRNFEPDGSVSGYGISHPGNL
                     YRKILRSRNNHMPRDEHEEIIGPFATGFYATAICEAAGVSVENGRTIDGVTRGTEIDV
                     AELLRRRIGNAADRYM"
BASE COUNT          494 a          737 c          959 g          551 t
ORIGIN      
        1 aagcgactgt cgtcacaacc aggacggccc aaggttgcgg cacttcggca gcaagggtga
       61 ttcctgcgcc cgccgcaagg atcacggcgt agatgatcag gtatttctct cttgttcgga
      121 tccggctcag catggtcttc atagttgccg gtcattctat ccaagtcttt ggggtttgtc
      181 ttgggggagg gcgtggttgg gatatgcccg cgctgcgttt gtcaagagcg gtgatcgcaa
      241 tagaataagt gcggtttcgt tgttccggac agcgaatgac gacatgaatg cttttgtggc
      301 cagggaggtt acccgtgttt ggtacgagga agtggcttgg cgttgtcctg cttgcggcaa
      361 ctattcagac gtcgtacttg gcggcccagc aaacggcacc ggcggggcga ggagctttgc
      421 gacgggagct gattgagcgt tttgacaagg atggtgacgg agagatcagc cgggaggagc
      481 gggcagccgc ccgcaagggg ctccggcaag gcggccgtgt acagcagaag cttgtcgaga
      541 ccgtgttgcc ggagagtgtg agggttgtgc gcgacgttga atacgcccgc gttggggacc
      601 agcctcttct actggacctt taccttccca agcccgaggg cgagccgttc ccagtggtca
      661 tctgggtaca cggcggagcc tggcaggcag ggagcaagga tctttgccgt gcggccttcc
      721 tgtcgggcga gggatatgcg gtggtcagca tcaactatcg gctgaccgat gttgcgccgt
      781 ttcccgctca gatccacgac tgcaaggccg ccgttcgctg ggtccgcgct cacgcaaagg
      841 aatacgggtt cgatccggct cggatcggcg tctggggctc gtcggcaggg gggcatctgg
      901 tcgcgctctt gggcacctcg gctgggatca gggaattgga gggcgatatt ggcggtaacc
      961 tcgaccagtc cagccgcgtg caggccgttt gcgatttctg cggaccgagc acgtttcgtg
     1021 cagaggatta tgagcatgaa ccggacaagc cgagaggggc cggtccgcag gtcgtgcaga
     1081 agcttcttgg cggtccgctc aataagaatc ccgaaaaggc ccggctggcg agtccgatcg
     1141 agcatgtcag caaggacgat ccgccgtttt tgatcgtgca cggcgagaag gataacgtcg
     1201 tgccagtgaa acacagtcgc ctgttggccg atgcgctgag gaaggccggc gcagacgtca
     1261 cgctgaaggt ggtccccgac gccggacatg gcgtcggcgg cttggctaac aggaagatgg
     1321 tggccgagtt cttcgaccag catctcaagc gcagacctgc gagccggcct gttgggagcg
     1381 cggctgaatg atgaggcgct atcgattctt gttgatggca acagccgttg cgacgttact
     1441 gtgggtcacg ggttgtgatg cgggtcgagg cgccggcggt tcggcagtca aacctgtccg
     1501 cacgctgacc gtggcggcgg tgtcttgtga aagccggatt catgaggttg agttcaatct
     1561 gtcgcggatc gaacactggg ccaggcaagc ggcggctgcg ggggccgaca tcgcgctgtt
     1621 tccggagacg ggcatcagtg gttggtgggc cagccgggag gtccgggcgt ttgccgagcc
     1681 gattgacggc ccggcgatca cccggttgac aaaggtggcg gatgagctgg ggatcgttct
     1741 cgccgtgggc atgaccgagc gtgacggcga caaggcgcat atcacgcacg ttgtgattga
     1801 cggcagaggc gtgatcggca tgcatcgcaa gaccagcctg gcaccgggcg aggaaaagac
     1861 atgggacccg ggcagtgatg ccaacgtgtt cgaggtcaaa ggcatccgtg ttgggatcgc
     1921 gatctgtttt gagtcggtgc atccgccaac ctgtgcgaga cttcgggaga acggggcaga
     1981 gatcatcttg gcgccgtatg ccaacggcac cgatccggac gaactgctca atggcaagcg
     2041 gccgtaccca tacgaacggg cgaaggagaa tcaggtctgg tatgttgcct gtgatgctcc
     2101 ggcacgcgat gagcagaaga acctcaggcg cggcgcggct tacgtgatca gtccggaggg
     2161 ggaactgaag gcgatcaccg ccccggatgc cgtgggtgag accatggttc tgtatacgat
     2221 ccgtctgtct tagtgctccc tttcatgaat ccttgtcggg ttgggcgctt gtattcgggc
     2281 gaaaaccgcg caaatcagcc cacaaagact tgtgaacggg agcgcttagt gcggcaggtc
     2341 tcggtcgccg cgggcgacct cgacaccgcc ctacgaccgc gggcttacat gtaccggtcg
     2401 gccgcatttc cgatgcgccg gcggagcagt tcggcgacgt cgatctcggt gccccgggtt
     2461 acgccgtcga tggtgcgacc gttctcgacg ctgacgcccg cggcctcgca gatcgcggtg
     2521 gcgtagaaac ccgtggcgaa cgggccgatt atctcttcat gctcgtcccg cggcatgtgg
     2581 ttgttgcgac tgcgcaggat cttgcggtag agattgcccg ggtgactgat tccgtagccg
     2641 ctgaccgagc cgtcgggctc gaaattgcga aagagcgggt tgcgttcctg gattccgttc
     2701 tcggtgatct cgcggcatcc cggcatgtcg atggccaggt c
//
LOCUS       DAOTPW010000403         2664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_29955_length_2664_cov_2.866616, whole genome shotgun sequence.
ACCESSION   DAOTPW010000403 DAOTPW010000000
VERSION     DAOTPW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29955_length_2664_cov_2.866616"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="P5316_21505"
     CDS             <1..1470
                     /locus_tag="P5316_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87591.1"
                     /translation="GGHLRFIQVPTETVLYEDGKDTGVYGYILAACIVIGQDERWRTI
                     EFIPRQTLTDDLVLRTKAYRKDGAFAGSATVTMNLPGFSKLTEIIQPPRFNSSGTQAV
                     GIVRYAKTGDQGGGVAVIVIDWASAQANVGVVKADSFDTVDYSAHSDQWSSYFWPDAG
                     FDFSGERQVSVPISVDYAGDAMQLLWTKYRRVYEEHRIASAAGTSNDLAESLSITHTS
                     SSTSTMELWLNDSVIKTATSSWHGDLSVSASLTRVITDPERPWLLRNSSVTSAGTWTK
                     TYSPFGSFITDVMADLRRGVIAYCVETDEGYSDTASESLSRTFTPGYPDTVMRNATRE
                     TPALTKTAVIEVMAGGATQVATVSGSVAEAVSFQSSASTPRIVAFGADNKIFFASLDA
                     EAYNRANQNTSGWEVSGYALNSAAVESLSMAVDKTGSTIFISGLLCNGLAKVFTPFAK
                     IIRAGGMSDMPVSEVISDDGQPAYTWVAEPIFLDIEVQP"
     gene            1467..>2664
                     /locus_tag="P5316_21510"
     CDS             1467..>2664
                     /locus_tag="P5316_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87592.1"
                     /translation="MKTLPKGPWLGINNRLPDFGLHKPKEGDFLRTAINVDVDDSGSL
                     RRRAAAVRVQAMTGAHSLFNGHLVRDSVLYRITLPTYSETMLKVLTSNAAMSYAEYNG
                     DIYFSNGTDSGRITQDGTVLPWAMPTPAAPAVSSVGGDLFAGKYQVVVSYANATTGEE
                     GGVSYPTQYQLSDAGGLRVTLPGAVAGATHIRVYVSAVNGSVPFLAGSFAVGTPVADI
                     IVAATGRDASVRNEVPLPAGTRIFMFNGCLCSIKGNDWFIGLPSRPGYRLDIGSRVPF
                     PAPISVAVSNQGGAYIVADRTYFFAGTDPASIDEVRDILPYGAVAGTEFEPPNKSLVG
                     WFGAKGVVLADAFGEVQTPMADNIDLTPPATGISTVFDTRGYRRVVSCGWCMNLDTKA
                     ATQYSGY"
BASE COUNT          542 a          832 c          792 g          498 t
ORIGIN      
        1 ggtggccact tacgattcat ccaagtcccg acggagacgg tgctgtacga agatggcaag
       61 gacactggcg tatacgggta catcctggct gcttgcatcg ttattgggca ggacgagcgg
      121 tggcgcacta tcgagttcat accccgtcag acactcaccg acgacctagt attgcggacg
      181 aaggcctatc gtaaagacgg ggcattcgct ggtagcgcca ccgtgactat gaacctgccc
      241 ggattctcca agctgactga gatcatccag ccaccgcgat tcaactctag tggcacgcag
      301 gcagtcggga tcgtccggta tgcaaagaca ggggatcagg gcggcggggt cgcggtaatc
      361 gtgatcgact gggccagcgc acaggccaac gtcggcgttg taaaggccga ttcattcgat
      421 accgtcgatt actctgcaca cagtgaccaa tggtcgagtt atttctggcc ggacgccgga
      481 ttcgattttt ctggagagcg gcaggtatcg gtcccgatct cagtcgacta tgccggtgac
      541 gcgatgcagt tgctctggac caagtaccga cgagtctatg aggagcaccg gatagcgtct
      601 gccgcaggaa cgtccaatga cttggcggaa tctctatcca tcacccatac gtcgagttcg
      661 acctcgacca tggagctatg gctaaacgac tccgtcatca aaacggccac gtctagctgg
      721 catggtgacc tgtcagtaag tgcctccctc acgcgggtta taactgaccc ggaacgccca
      781 tggctgctaa ggaattcctc tgtgacctcg gcaggaactt ggacaaagac gtactccccg
      841 ttcggatcgt tcataaccga tgtcatggct gatttgcggc gaggagtaat agcgtactgc
      901 gtcgagaccg acgaagggta ttcagacacg gcctctgagt cgttgagcag aacctttacg
      961 cccggatacc cagacaccgt gatgaggaac gcgaccagag aaacccctgc gctcacgaaa
     1021 acggcagtga tcgaggtaat ggccggcggg gcgacccaag tcgctacggt ctccggcagc
     1081 gttgcagagg cggtcagctt tcaatcgagc gcaagcacgc cgagaattgt tgcattcggt
     1141 gcggacaata aaattttctt cgcttctctg gacgcggagg cctacaacag ggcgaatcag
     1201 aatacatccg gctgggaggt gtcagggtac gcgctcaact cggcagccgt cgagtcgctg
     1261 tccatggccg tcgataaaac cggcagcacc atctttatct ccgggctgct gtgcaacggg
     1321 ctggcaaaag tattcacccc gttcgccaag atcattcgcg cgggcggcat gagtgatatg
     1381 ccggtttccg aagttatcag cgacgacggg caaccggcat acacctgggt cgctgaaccc
     1441 atctttctcg acatcgaggt ccaaccatga aaactctacc gaaaggcccg tggctcggga
     1501 tcaacaaccg tctgccggac ttcgggctgc acaagccgaa agagggcgac ttcctgcgca
     1561 cggcgatcaa cgtcgacgtc gacgactccg gcagcctgcg gcggcgggct gcagccgtcc
     1621 gcgtacaggc gatgaccggc gcgcactccc tgttcaacgg gcatctggtg cgcgactccg
     1681 tcctgtaccg catcaccctg ccgacctaca gcgagaccat gctcaaggta ctcacgtcga
     1741 acgccgcgat gagctacgcc gagtacaacg gcgacatcta cttttcgaac gggaccgact
     1801 ccggacggat cactcaggac ggcaccgtct tgccgtgggc catgccgacg cccgccgctc
     1861 cggcggtatc cagtgtcggc ggagacctgt tcgccggtaa ataccaagtg gtggtcagct
     1921 acgccaacgc gaccacaggg gaagagggcg gggtcagcta cccgacgcaa taccagctga
     1981 gcgacgctgg cgggctgcgg gtcactctgc ccggggccgt cgccggtgcc acccacattc
     2041 gggtgtatgt ctccgcggtc aacggctcgg tgcccttcct ggccggctcc ttcgcagtcg
     2101 gcacgccggt ggcagacatc atcgtcgccg ctaccgggcg agacgccagt gtccgcaacg
     2161 aggtgccgct gccggctggc acccgcatct tcatgttcaa cgggtgcctg tgttcgatca
     2221 aggggaacga ctggttcatc ggcctgccgt ctcgccctgg ataccgccta gacatcggct
     2281 cgcgcgtccc tttccccgcg ccgatcagcg ttgctgtatc caaccagggc ggggcgtata
     2341 tcgtcgccga ccgcacctac ttcttcgctg gcaccgaccc ggcaagcatc gatgaagtcc
     2401 gcgacatcct cccctacggc gcggtggccg gtacggagtt tgaacccccg aacaaatcgc
     2461 tcgtcggctg gttcggcgcc aaaggcgttg tactcgcgga tgccttcggc gaagtccaga
     2521 cccccatggc cgacaacatc gacctgaccc cgccggccac cggcatctct acggtcttcg
     2581 acacgcgcgg ctaccggcgg gtggtgtcct gcggatggtg catgaacctt gacacgaagg
     2641 ctgccaccca atacagcggc tacg
//
LOCUS       DAOTPW010000404         2633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_30418_length_2633_cov_4.407680, whole genome shotgun sequence.
ACCESSION   DAOTPW010000404 DAOTPW010000000
VERSION     DAOTPW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30418_length_2633_cov_4.407680"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="P5316_21515"
     CDS             complement(<1..1609)
                     /locus_tag="P5316_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87593.1"
                     /translation="MRAQTPITHHMDAVAGRPLDEGRCSRRAGLLAGLVICAGVASHL
                     PSLRWGFLYDDYIHQFMLRRIEHGDPARVWNLFDFGVRPEPGHPLFEWGFYPWWTDAD
                     FKIRFFRPVTSLSIAADHWLYGDWAPGYHLTSLGLFAVLLVMVYRLYRALELPGSALA
                     WAMAIMALSDIHSLPVGWIANRNALWVGVFLVGMLLSLHAYWARAGHTMLVSAVVCFL
                     LACGAKESGIIGLPVAVLHEWLYPRAASAPSLRGRLAAVVKSRPLLAIVLAAGAYVCW
                     YASAGYGTQSLLYPTPWRDPGQYFSRLATIMPLAGVSLFVGFSADIAAALRDTMPWVL
                     PAAAIVSIAVFWILARTIRWTPAAFLALGLAVFSFIPEAGADPSDRLFLNTSIGTSML
                     IALLLHHVGRWSIALRERRIARLTLAAIVVFRGLLLPVPGTLLRGYFFSSMGGLDRAA
                     IAAADIDRERPEPRHVLVFNSPSSLLSTFQLATWAVMHDDAGTRIHLVQMGRRGLQWH
                     RQDDRRMTLTSLGTPLGDQRFERLFRTG"
     gene            complement(1613..2446)
                     /locus_tag="P5316_21520"
     CDS             complement(1613..2446)
                     /locus_tag="P5316_21520"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRR87594.1"
                     /translation="MEHSQTSSPLRAGSQAQMDPQARKSADKRGFLWGFLLLDLWVRL
                     TLWSTSLGLTTAVMTALQAWPAGNPLAGDPGLTMLWAKRIGQWVILFNVIYLLELLLV
                     RLPIPTPREGVYSTKGFPDRQLVWITLLGVLTKARYQVPFPGFLVFHLANLPPLCWLV
                     SRIFGPKSQSCYATEPEILDPSLVEIGRNVIIGYGTRIVAHIQEADKVTIQKTIIEDD
                     VLVGGEVSILAGVRLRKGCVIGARSLLLPGTVVGPHEFWAGIPAKRISSLQQESPAQG
                     T"
BASE COUNT          575 a          850 c          799 g          409 t
ORIGIN      
        1 gacccgtgcg gaaaagacgc tcgaaccgct gatcgccaag cggtgtgccc agcgaggtca
       61 gtgtcatccg gcgatcatcc tgacggtgcc actgcagccc gcgccggccc atctgtacca
      121 ggtgaatccg cgttccggca tcgtcatgca tgaccgccca agtcgccaac tggaaggtgg
      181 acagcaggct ggaggggctg ttgaaaacga ggacgtgacg cggctcgggg cgttcgcgat
      241 cgatgtcggc ggcggcgatg gccgcacgat ccagcccccc catgcttgag aagaagtagc
      301 cgcggagcag cgtacccggc acgggcaaca gaagcccacg gaacaccacg attgcagcca
      361 gggtcaaccg tgcgattctt ctctcgcgca gcgctatgct ccatcgcccg acatggtgca
      421 gcagcagagc gatcagcatc gaggttccga tcgaggtgtt gaggaacaag cggtccgaag
      481 gatcggcgcc ggcctccggg atgaatgaga acacggccag gcccaacgcc aggaacgccg
      541 ccggtgtcca gcggatggtg cgagccagaa tccagaagac tgcgatcgag acgattgccg
      601 ccgccggcag cacccatggc atggtgtccc gcaaagccgc ggcaatgtcg gcgctgaacc
      661 cgacaaacag acttacgccg gccagcggca tgatggttgc cagccgcgag aaatactggc
      721 ccgggtctcg ccagggagtc ggatacagaa gtgactgcgt tccgtagccg gccgacgcgt
      781 accagcagac ataggccccg gcggcaagaa cgatggccag gagcggtcgc gacttgacca
      841 ccgccgcgag ccggcctcgc aacgacggcg cggacgccgc tcgcgggtag agccattcgt
      901 ggagaacggc caccggcagg ccgatgatcc cggactcctt cgcgccgcat gcaagcagaa
      961 agcagacgac cgccgacacc agcatcgtgt gccctgcccg cgcccagtaa gcatgtagcg
     1021 acaggagcat cccgaccagg aacacaccca cccacaaggc attgcgattg gcgatccagc
     1081 cgaccggcag actgtgaatg tcgctcaagg ccatgatggc catcgcccag gcaagagcgc
     1141 tccccggcag ctctagtgcc cgatacagtc gataaaccat caccaataag actgcaaaaa
     1201 gccccaggct cgtcaagtgg tagccgggcg cccagtctcc atatagccag tgatccgccg
     1261 cgatgctcag actggtgacc ggccggaaga accggatctt gaagtccgcg tccgtccacc
     1321 agggatagaa accccactcg aacagcgggt gtcccggttc ggggcggacg ccgaagtcga
     1381 acaggttcca gacccgcgcg ggatcaccgt gctcgattcg ccggagcatg aactggtgta
     1441 tgtagtcatc gtacaagaac ccccaccgaa gcgaaggcag atgcgaggcc accccggcac
     1501 agatcaccaa tcccgccagc aacccggctc gccgagagca gcgcccctca tcaagaggcc
     1561 ggccggcaac agcgtccatg tgatgggtga tcggcgtttg cgctcgcatg tctcaagtac
     1621 cttgcgccgg gctctcctgc tgaagggaac tgatacgctt ggccggaatc cctgcccaga
     1681 actcatgtgg accgactacc gttcccggca acagcagcga tcgggcaccg atgacacatc
     1741 cctttcgcag ccgcaccccg gccagaatgc tgacttcgcc tcccacgaga acgtcgtcct
     1801 cgataatggt cttctgaatg gtgaccttgt ccgcctcttg gatgtgagcg acgatccggg
     1861 tgccgtaccc gatgatcaca tttcggccga tctccaccag cgaaggatcc agtatctcgg
     1921 gctcggtcgc atagcacgat tgggacttcg ggccgaagat ccgactgacc agccagcaca
     1981 acggaggcag attggccaga tggaaaacca ggaaaccggg aaaaggaact tggtagcgcg
     2041 ccttggtgag cacccccaga agagtaatcc agaccagttg tcggtcggga aaacccttcg
     2101 tcgagtagac cccttccctg ggcgttggaa tgggcaagcg gacaagaagc aactccagga
     2161 gatagatcac gttgaaaaga atcacccact gtccgatgcg ttttgcccag agcatcgtga
     2221 ggccaggatc accggccagg gggttgcccg ccggccaagc ctgcaacgcc gtcatcacgg
     2281 cggtcgtcag gcccaacgag gtggaccaca acgtcaagcg aacccagagg tccagtagga
     2341 gaaaccccca taggaatcct cttttgtcgg ccgatttcct ggcttgcggg tccatctggg
     2401 cttgagaccc ggcccgcaaa ggcgaagacg tctggctgtg ctccacggtc atggcctccc
     2461 tggacaacca cactcgcgac gcgcggcctt gcgcgggcgt caacgcgaaa accgtttatt
     2521 cgatgtagcc cagttcctgc aaatgccgct cgatgctttg ctgctgttcg gcggtaatct
     2581 cgagcatctc cgccggggct tggccctcgg ccggcaacat gctctcggtg agt
//
LOCUS       DAOTPW010000405         2633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_30424_length_2633_cov_3.479829, whole genome shotgun sequence.
ACCESSION   DAOTPW010000405 DAOTPW010000000
VERSION     DAOTPW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30424_length_2633_cov_3.479829"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="P5316_21525"
     CDS             complement(<1..1063)
                     /locus_tag="P5316_21525"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRR87595.1"
                     /translation="MERTGILSRSILAVAILVAAAPAGWAAITVTSNGPMQQINTAGI
                     PGSYMREFSTIWESGNPNAGAIFYVDSASSPYTLYRASTTDGGLTWTAGQPAGLARPA
                     GVASQNQQNPVIRDFNRDGNLTGFFGSVQGVSSGLPGYPNTLHYRAVSTATPGNIGPT
                     WSGETLISYNSGAFPGQTGLNGFIEVFETSSGTLRGYLAGNRTVNLTSTTHLVESTDG
                     GLTWQDMGLIQIPGPGTAFNTVGANGPVFWFTDDADGQRKMGWFCYGEGSNNRGVAFL
                     ISTDEGLSWTKQASIFNLVDATIRSGDANFISDDTVRLFYYRNTGSVPSDRQLWYQDF
                     TLTGMSGIVANNLTLGAAPI"
     gene            complement(1221..1643)
                     /locus_tag="P5316_21530"
     CDS             complement(1221..1643)
                     /locus_tag="P5316_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87596.1"
                     /translation="MSWYRGPLEQEMAGELLWLGCGDEVQLLICDGRGRTQAVQRGSG
                     DGEDGFDSGLRAVSSRGLSEALEAGPQTAWLYDLPAELGAEVTVVNPNKVRLIAEGRR
                     KTDKIGVETMRRVRMGRHPPSVRQKAGRSTGKSLLRRG"
     gene            complement(1704..>2633)
                     /locus_tag="P5316_21535"
     CDS             complement(1704..>2633)
                     /locus_tag="P5316_21535"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRR87597.1"
                     /translation="IRQLCAAGRLKTMPGVGTFVASGTSLDGVVMVIRLDSAEMENTT
                     AVAHDSIEGAQAACVEAGLPLVLTRSDVDPEKYLRLRYGFLVVIGAANDADAARWAQI
                     LHTACRPFVSIGVDKGWANHIQPDGGRIAQSAVEHLYQSGHRRIALVPRVSFAGKLRF
                     GPEQVIPHPDLALGVYPFETTGGWDLCRANLEEAMGRAMSEWQPTGIVVGPDQFALFG
                     LDYLANRGLRVPEDLSVVTCCPSAFSEWRGLRITRVDNPHREVARRAVEELIKMGGGG
                     GYSCGRIAVAPALFQGETSRLITGPAGSPRSGT"
BASE COUNT          458 a          926 c          790 g          459 t
ORIGIN      
        1 ggatgggcgc ggcgcccaag gtcaggttgt tggcgacaat gccgctcatc ccggtcagcg
       61 tgaagtcctg gtaccagagc tggcggtcgg acggcacgct gcccgtgttg cggtagtaga
      121 acagccgcac cgtatcatcg gagatgaaat tggcgtcgcc gctgcggatg gtggcgtcga
      181 ccaggttgaa gatgctggcc tgcttggtcc aggagaggcc ctcgtcggtg ctgatgagaa
      241 aggcaacgcc gcggttgttc gagccctcgc cgtagcagaa ccagcccatc ttccgctggc
      301 cgtcggcgtc gtcggtgaac cagaacaccg ggccgttggc tcccacggta ttgaaggccg
      361 tccccggacc tgggatctgg atgagcccca tgtcttgcca ggtcagcccg ccgtcggtgc
      421 tctcgaccaa gtgagtcgtg ctggtgagat tgactgtcct gttgccagcc agataccctc
      481 gcagagtgcc cgacgaggtc tcgaacacct cgatgaaccc gttgagcccg gtttggccgg
      541 ggaaggcccc gctgttgtaa ctgatcagcg tctcgcccga ccacgtcggg ccgatgttgc
      601 ccggcgtcgc cgtcgagacg gcccggtagt gcagcgtgtt gggatagccg ggcaggccgc
      661 tggatacgcc ctggacgctg ccgaagaaac cggtcaggtt cccatcccga ttgaaatcgc
      721 ggatgaccgg gttctgctga ttctgcgagg ccacgccggc cggccgggcc aggcccgcag
      781 gctgccccgc cgtccacgtc aatccgccgt ccgtggtcga ggcccgatac aacgtgtagg
      841 gcgacgacgc ggaatccaca tagaagatcg cccccgcgtt cgggttgccc gactcccaga
      901 tcgtagagaa ttcccgcatg tacgagccgg ggatccccgc cgtgttgatc tgctgcatgg
      961 gaccgttgct cgtgaccgtg atggcggccc aacccgccgg agccgccgcg accagaatcg
     1021 ccaccgccag aatcgatctt gaaagaatcc ccgtgcgttc cataaccacc tcctttgtgc
     1081 agaatcaccg tgcacacgcc gctcgattgc cgagggcgaa gaaaccaccg cagacagtat
     1141 attcgctcgc cggtcacccg tctgtcaagc gaaaaaacct gccacgcgcg gcggggcggg
     1201 ccggggtcga cagccttcgg ttaccctcgg cgcagcaagg atttacccgt agaacggccg
     1261 gccttttgcc gtaccgacgg cggatggcgg cccatgcgga cacgacgcat cgtctcaacg
     1321 ccaatcttgt ccgtcttccg ccggccctcg gcgatcagcc gcaccttgtt cgggttcacc
     1381 accgtcacct cggcccccag ctccgccggc agatcgtaga gccacgccgt ctgcggcccg
     1441 gcctccagag cctccgacaa cccccgcgaa gacaccgccc taaggccgct gtcgaagccg
     1501 tcttctccgt cgccacttcc ccgctgaacc gcttgtgtcc ttccccgtcc gtcacaaata
     1561 agcaactgga cttcgtcgcc acatccaagc cacaatagtt cgccagccat ctcctgttcc
     1621 aaaggtccac gataccagga catcttccaa tgcctcctat cgtaaccgga gtcggagtag
     1681 ctcacttcgc agcttttcct ttcctaagtt cccgacctcg gggaaccggc tggcccggtg
     1741 atcagtcggg acgtttctcc ttggaacagg gctggggcca cggcgatccg gccgcacgaa
     1801 tagccgccgc ctccgcccat cttgatcaac tcctccaccg cgcgccgggc cacctcccgg
     1861 tgcgggttat ccactcgggt gatcctcagg ccccgccatt ccgagaaggc cgacgggcag
     1921 cacgtaacga ccgagaggtc ctccggcacg cggagacctc ggttcgccag gtaatccagg
     1981 ccgaagaggg cgaactggtc cggcccgacc acgatccccg tcggctgcca ttccgacatg
     2041 gctcgtccca tcgcttcttc aaggttggcc cggcacaggt cccagccgcc cgtggtctcg
     2101 aacgggtaga cccccagcgc caagtcggga tgtgggatga cctgctccgg cccgaatctc
     2161 agcttgccgg caaacgacac ccgcggcacc agggcgatgc ggcgatggcc ggattgatag
     2221 agatgctcca cggcactctg ggcgatccgg ccgccgtccg gctgaatatg gtttgcccag
     2281 cccttgtcca ccccgatcga gacgaacggc cggcaggcgg tgtgaaggat ctgggcccaa
     2341 cgcgcggcgt cggcgtcgtt cgcggcgcct atcaccacca gaaacccgta tcgcaaccgc
     2401 aggtacttct cgggatccac gtccgaccgg gtgaggacga gcggcaggcc cgcttccacg
     2461 cacgccgcct gggccccctc aatgctgtcg tgggccaccg cagtcgtgtt ctccatctcc
     2521 gcagagtcca ggcggatcac catgaccacg ccgtccagcg acgttccgct cgccacgaac
     2581 gttcccactc cgggcatggt cttcaaccgt ccggcggcgc acaactgtct gat
//
LOCUS       DAOTPW010000406         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_30826_length_2607_cov_5.894592, whole genome shotgun sequence.
ACCESSION   DAOTPW010000406 DAOTPW010000000
VERSION     DAOTPW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30826_length_2607_cov_5.894592"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="P5316_21540"
     CDS             <1..325
                     /locus_tag="P5316_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87598.1"
                     /translation="RIELRGLSEGNCLIPRAVDPRNAGCGDAHITASVSRLQYRRILT
                     ENMPDHAAKRWHSQLVSPILVAYIVVVRGPASGERRPTGARNPNRRSGGPYPGRSGTG
                     IWPVK"
     gene            394..1581
                     /locus_tag="P5316_21545"
     CDS             394..1581
                     /locus_tag="P5316_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87599.1"
                     /translation="MGFTLRNLRFRRTCHQHNVLAGIFVAVLAGIGGCDLTSWVDNLG
                     TDGNVGLNPSLFPERRIQGEPNDAFDNALDVIFDSAGRAHLAGTITHSDDVDVYLLGS
                     LEPGDRIIVDVGTPGSLLDAVIVIFDAEGRLTFENDDRNYALRQYDPFLNQVVRHSSP
                     KYYLAITAAPLGSGYASRGTYDVLITVTSGNDVPAPAGQVVVLDFDGGSVTIPDYGSY
                     KLQPFDAADIDPVYAGQTALLRQLIAETVRENYEGLNLDVRVIPDDPLPAGEYSSIFF
                     GASSSDAFGVAEAIDPYNMDRDDDAVVFTNLFTKRLFGRVLTIEELGTAIGNVASHEM
                     GHLLGLNHVADIYDLMDTTGGAGTLLIDQEFLRDSPLDSSVFPFGIQDAWLWLMETLG
                     PAS"
     gene            complement(1601..2272)
                     /locus_tag="P5316_21550"
     CDS             complement(1601..2272)
                     /locus_tag="P5316_21550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87600.1"
                     /translation="MKHRVQGLAMCLGFLACACSLQAAMISVTVDPMYGSAGNTGATA
                     LIEFSFVEDGPDDLLVVAIHNTTPASIGSKLTAVGFEWPASLALPLSYAEGGQSAYFD
                     EIGFHVNVSPFWLNASGGYDVMITSDGNFEGGSPLGAPSAGETQSIILNLGDTGLNPD
                     QLSATLGDWYADPERVSVIVRFQAVAGDRSDKVISRTPEPAGMLLLGLSQLALCARRR
                     TRRHP"
BASE COUNT          517 a          839 c          748 g          503 t
ORIGIN      
        1 caggatcgag ctgcgcgggc tctcggaggg caactgcctg atccctcgtg ccgtcgaccc
       61 gcgcaacgcc ggttgcggag atgcgcacat taccgcgtcg gtgtctcggc tgcaatacag
      121 gcggatcttg acggaaaaca tgcccgatca tgccgcgaaa cgctggcatt ctcagttggt
      181 ctcgccgata cttgtcgcgt acatcgttgt tgtacggggg ccggcctccg gggagcggcg
      241 accgacgggt gcccgaaacc cgaaccgtcg aagcggcggc ccttatcccg gtaggtctgg
      301 caccggcatc tggccggtca agtgacaagc tggaagcccg gtccgcacgg cccgccaaca
      361 taggccacta gtaaaaacac gggggcatga tccatgggct ttaccctccg aaatctcaga
      421 ttcagacgca cctgtcatca gcataacgtc ctcgccggta tcttcgtcgc cgtcctggcc
      481 ggtatcggcg ggtgcgacct cacaagctgg gtggataacc tgggaaccga tggaaacgtc
      541 gggctcaacc cgtctctttt tcccgagcgg cgcatccaag gtgagcccaa cgacgccttt
      601 gacaacgcac tcgacgtgat attcgactcc gcgggccggg cccatctcgc cggtaccatc
      661 acccattccg acgacgttga tgtctacctg ctgggctccc tggaaccagg cgacaggatc
      721 atcgtcgacg tcggtacacc cggcagcctg ctggatgcgg tcatcgtcat cttcgacgct
      781 gaggggcgac tcactttcga gaacgacgat cggaattatg cccttcgcca atacgatccc
      841 ttcctgaacc aggtcgtccg gcattcaagt ccgaagtact acctggcgat caccgcggca
      901 ccgctgggat cgggctacgc gtcgcgagga acctacgacg tcctcatcac cgtgacgtcg
      961 ggcaacgatg ttcccgctcc cgccggacag gtggtggttc tggattttga cggcggttcg
     1021 gtcaccattc ccgactacgg ctcatacaag cttcagccgt ttgacgccgc cgacatcgat
     1081 ccggtttacg ccggccagac ggccctcctg cgccaactca tcgcggaaac ggttcgggaa
     1141 aactacgagg gattgaacct cgatgtgcga gtgatcccgg atgacccctt gccggcgggt
     1201 gaatactcca gcatattctt cggcgcaagc agcagcgatg ccttcggggt ggccgaagcc
     1261 atcgacccct ataatatgga ccgcgacgac gacgcggttg tcttcaccaa tctgttcacc
     1321 aagagacttt tcggccgggt cttgacgatc gaagagctgg gaacggccat cggcaacgtt
     1381 gcttcgcacg aaatgggaca cctgctgggc cttaaccacg tcgcggacat ttacgacctg
     1441 atggacacca ccggcggggc aggcacatta ctgattgacc aggaattcct gcgggattcg
     1501 ccgctcgaca gcagcgtgtt cccctttggc attcaggatg cctggctctg gttgatggaa
     1561 acgctcggac cggcttcttg agagcttttt ccgggccgcc tcacggatgc ctgcgcgtcc
     1621 gcctgcgcgc acaaagagcg agttgcgaaa gaccgaggag cagcatgccc gccggctccg
     1681 gggtgcgact gataaccttg tcggaacgat cgccggcaac tgcctgaaac cggacgataa
     1741 ccgagacacg ctccggatcg gcataccagt cgccaagtgt ggcgctcagt tggtccgggt
     1801 tcagccccgt gtcgcccaga ttcaatatga tgctctgcgt ctcccccgcg ctcggtgccc
     1861 ccagcgggct cccgccttcg aagttgccgt cgctcgtgat catgacgtcg taaccgccgg
     1921 aagcgttcag ccagaagggt gagacattca cgtgaaagcc tatttcgtca aagtaggcgc
     1981 tctgcccgcc ctcggcatag gacagcggca aggcaagcga tgccggccac tcaaaaccca
     2041 cggcggtgag cttcgagccg attgaagccg gtgttgtgtt gtgaatcgcc acgaccaaca
     2101 ggtcgtcggg accgtcctca acgaagctga attcgatgag cgccgtcgcc cccgtgtttc
     2161 ccgccgaacc gtacatcggg tcgacggtta cactgatcat cgccgcctgc aaggagcatg
     2221 cgcacgcgag aaagccaaga cacatcgcaa gaccttgaac acggtgtttc atctcttccc
     2281 cccctttgca tgctatccgg cgaaaaccca aaaggtccgg gtcgatcggc cgcttctatg
     2341 catatatgca cagagcacac ccgatgtcca ggtcccataa cgaacttttc caaacgggca
     2401 gataatcttc ctgaagcgga ccgctcgccc gtgccggcga tccggttcca ctcaagaagc
     2461 tcgatgaggc cttgcctcgc tggtctccgg cgtttgccgc ggccgacgac caggtgtcga
     2521 gggcggccga agacctgcaa tacctcagtg gacaaatctg gctggtcagc cactatctct
     2581 tcagaaaccg accgcccggc ctctttt
//
LOCUS       DAOTPW010000407         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_30827_length_2607_cov_5.300157, whole genome shotgun sequence.
ACCESSION   DAOTPW010000407 DAOTPW010000000
VERSION     DAOTPW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30827_length_2607_cov_5.300157"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="P5316_21555"
     CDS             <1..893
                     /locus_tag="P5316_21555"
                     /inference="COORDINATES: protein motif:HMM:NF024308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HRR87601.1"
                     /translation="YQTREVHSPQFSEMGMAMSPHGWLYPYLRILGSLMRSLPFSQMT
                     LHPELAGNGVCLAQVPHRYVCYAPNGGTITLNLSASSGTLQARWLNPRAGGFGSPVPV
                     QGGQNRTFAAPDANDWVLYVEDSAVADTIAPGAPAAFTAAAGRLQNTLTWTNPHDGDV
                     TGVLIRFSTSGYPAGPTDGQLLADEPGEPQWPYTVLHVGLTRGITYYYSAFAHDWMMN
                     HGPAAHAQAIPVGPADFDWDGDVDQTDFGHFQACISGSGVALSPGCEDADLAPDGAVD
                     QNDLSVFRTCMGGSDQPPGC"
     gene            1228..2418
                     /locus_tag="P5316_21560"
     CDS             1228..2418
                     /locus_tag="P5316_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87602.1"
                     /translation="MSRRTFASVGLCCLLWLVAGTARIDLAAAAALPPSADEETQART
                     QLRSTPVGAVPKRVPLIYGTDLFHPHDDPDDHFDLATVFAMPELDVRAILLDLGKRQA
                     EKPGRIPVEQMFKLTGRRVPYAVGLSETLKSPQDQGLDQPEEYQAAVELLLKTLRDSP
                     EPMILHTTGSVRDVCAAYNRDPELFRRKVTRLYVNIGNLLENKNEWNVHLDVPAYVGL
                     MRSGLPIYWCPCLPIKENRSTHWAFKHGQVLEGVPLPLLNYFVYALQTVRPNEIDPID
                     AMNMDLRPWRHILMGMERNMWCTGPLIHTAGRTVHRVGDRWVAAASPPPGATPADVFT
                     FVPVRVEISDKGYTKWVENDPKPNMHLYKVTDPKNHEPALKSCLHDLFVRFPVVIQSD
                     LKPD"
BASE COUNT          489 a          867 c          747 g          504 t
ORIGIN      
        1 cttaccagac ccgggaggtc catagccccc agttcagcga aatgggtatg gccatgtccc
       61 cccacggctg gctctacccg tatctccgca tcctcggaag cctcatgcgc tcgcttcctt
      121 tctcgcagat gactcttcac ccggaattgg ccggcaacgg cgtttgcctg gctcaggttc
      181 cccaccggta cgtgtgctac gctcccaacg gcggcacgat cacgcttaac ctgagcgcct
      241 cgtccggcac gcttcaagcc cgttggctga atccccgcgc aggcggcttc gggtcgcccg
      301 ttcccgtcca agggggacaa aaccggacgt ttgccgctcc agatgctaat gattgggtgc
      361 tgtatgtcga agactccgca gtcgcggata cgatcgcgcc gggagcgccg gccgctttca
      421 ccgcagccgc aggaaggctt cagaacacgt tgacttggac caacccgcac gatggcgatg
      481 tcaccggtgt cctcatacga ttcagcacat ccggttatcc ggccggcccg accgacggcc
      541 agttgctcgc cgatgaaccc ggcgaacccc aatggcccta cacagtgctc catgtaggtc
      601 tgacgcgcgg cattacgtac tactactccg ccttcgccca cgactggatg atgaaccatg
      661 ggccggccgc acacgcacag gccatacccg tgggccccgc ggactttgac tgggacggag
      721 acgtcgatca gacagacttc ggtcacttcc aggcctgtat ttccggatcg ggagtcgctc
      781 tgagtcccgg ctgcgaagac gctgatctag cacccgacgg cgccgtggat cagaacgatc
      841 taagcgtttt cagaacctgc atgggcggat cggaccaacc cccggggtgt tgaactcctc
      901 ggtagtcctg tcggcctgtg tcacggcatc gaaggcaagc caaccccgac cacgtgtgcc
      961 tccaccatgt gctggtcgtc gagaatgaga atccagacca cccccgaccc tcgcggccga
     1021 gcagcttccc gaacgaccgc gagcactttc cgagtctgtc gtggtaatca tcacgggcca
     1081 agccgtcgcg ttaccggcat cgagggcctc gacaatcatc ggggcttacc gtgaggcgcg
     1141 attgctggat ggctgacggg cgttatgatg ccgggcgcct ggaaccgggg caggcaacag
     1201 atagcaccct gagcgtggag atgaatcatg agcagacgaa cctttgcatc agtcggcttg
     1261 tgctgtttgt tgtggctggt tgcaggtacc gccaggatcg acctcgccgc agcggccgcc
     1321 ctgccgccca gcgccgatga agaaacacag gcccgtacgc aacttcgctc cacccccgtt
     1381 ggagcggtcc ccaaacgcgt ccccctgatc tacgggaccg atctgttcca tccgcatgat
     1441 gatcccgacg accactttga tctagccact gtcttcgcca tgccggagct ggatgtgcgg
     1501 gctattctcc tggacctcgg gaagcggcag gccgaaaaac cgggccgcat ccccgtcgaa
     1561 cagatgttca aactgaccgg ccgaagagtg ccctatgccg tcggcctcag tgagactctc
     1621 aagtcgcccc aagaccaggg cctcgatcaa cccgaagagt atcaggcggc ggtcgagctt
     1681 ctgctgaaga cgctgcgcga ctcaccggag ccaatgatcc tgcataccac tggcagcgtt
     1741 cgcgacgtct gcgccgccta taaccgtgac cctgagctgt ttcggcgcaa ggtaacccgc
     1801 ctgtacgtca acatcggcaa tcttctcgag aacaagaacg agtggaacgt tcatctggac
     1861 gtccccgcct acgtcggcct tatgcgctcc ggcctgccca tctactggtg tccctgcctg
     1921 ccaatcaagg agaatcgcag cacccactgg gctttcaagc acggacaggt cttggaaggt
     1981 gtcccgctgc cgctgttgaa ctacttcgtc tacgctctcc agactgttcg ccccaatgag
     2041 atcgatccca tcgacgcaat gaacatggat ctgcggccct ggcgccacat cctgatgggc
     2101 atggagcgca acatgtggtg caccgggccc ctgattcaca cggccggacg cacggttcac
     2161 cgcgtgggcg accgctgggt ggcggctgca tccccgcccc cgggagcaac gcctgcggac
     2221 gtgttcacgt tcgtgccggt gcgcgtcgag atcagcgaca agggatacac caagtgggtg
     2281 gagaacgatc caaagccgaa catgcatctg tataaggtca cggatccgaa aaaccacgag
     2341 ccggccctga aaagttgtct ccacgatctg tttgttcgtt ttccggttgt gattcagtcc
     2401 gacctgaagc ccgattagtc tcttccgtcg cgactggtgc gcattttgcg cgcatgtctc
     2461 ccgatcgtcg ggtgccatgc ccacggcttt gcgtgggcat gcggaggtag gaccgctcgc
     2521 cccggaatcc tttccgggcc ctggagtttg gcattccgct cggcccggaa tcctttccgg
     2581 gccgcatccc ctgcggaatc ctttccg
//
LOCUS       DAOTPW010000408         2582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_31236_length_2582_cov_4.194302, whole genome shotgun sequence.
ACCESSION   DAOTPW010000408 DAOTPW010000000
VERSION     DAOTPW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31236_length_2582_cov_4.194302"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2522)
                     /locus_tag="P5316_21565"
     CDS             complement(<1..2522)
                     /locus_tag="P5316_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87603.1"
                     /translation="MNPSSYNCDIGVLEQTVSVVPGYYNLALSFWAWVYDTGAWPSVV
                     AGELLVDDVVVAAAVKSSTDGVPPSQSYQQVWATWAGEVASGWVKVRFTLVGDGAGGT
                     GWGIAALDDVELFACSEGTCAGQHDVTDIEPNVISTPVATDTTVTITGTNLTNVTGVH
                     LWKTQVGMWNNNGANGVLIAGTITAQTATSLTVSLATAGQAAGVFDLVVEQAGVNPVV
                     ISNAFEILDPSVPQNLLANPSFEVVNVGSDGKPLAPMDAVGNAAMWFGDYVKNPAANP
                     NPTSIDYRTDYFGIYGIWSTSRGHPGTSTTSMWQTVPIAPGSAVSFSGWLDCNRFGTS
                     QAKAKVTLYDGDKTGSTIGSRSIDHTTAGAGGDYGTWVGVTINGTSYSGYVTVELATE
                     LVCPGYSAAGAFADAFSLTASPACTGPLHPWIAYPVRGPTNVDPTLVITGGSNLDEVS
                     AVKLVFVDRKASGDQAETVVPGTILTHSPTKLIVSFPMHTSGAHADLYNLVLEKPNAP
                     PANLEFYRVRRSTGAGSLRFHDNVPRTFVDAFDLFCVDPVTLTGVSPSAIVVREGQTE
                     LKITGTNVTVLADSANSIKLVQGATELVATNVVPSGSDVVARFDFASAPAGFYDLVAV
                     RGDQCDSPAPLPLAFRYSPPGSQLLPNGDFELEGPKGGDATPVAFWTSYETAVPVRYN
                     NQSWVPSPSTQGGSNRGGISEGGVGASMQRVIQTVPVLPRYELTLTGWIYGGASAGLI
                     HDHQVLIRDGGPNGPIIGFFSVTDPASVWTPFTIKAAPTTSEVTVEWGHVPDSGNPGN
                     PPGTVATHVDELFLVQSASPCSFPVAADVDGEPARRLREVLAS"
BASE COUNT          489 a          851 c          777 g          465 t
ORIGIN      
        1 gacgcgagga cctcgcgcag ccttcgcgcg ggctcgccgt cgacgtcggc cgccaccggg
       61 aaagagcagg gagaagccga ttgcaccaga aacagttcgt cgacatgggt cgcaacagtc
      121 cccggcgggt tgcccggatt ccccgaatca ggcacgtgcc cccactccac cgtcacctcg
      181 gaagtggtcg gtgcggcctt gatcgtaaag ggggtccaga cgcttgccgg atcggtgaca
      241 gagaagaaac cgatgatggg gccgttcggc ccaccgtcgc gaatgagtac ctgatgatcg
      301 tggatcaggc ccgcgctcgc accaccgtaa atccaacccg tcaacgtgag ctcatagcga
      361 ggcagaacgg gcaccgtctg aatcactcgt tgcatcgacg ccccgacgcc tccttccgag
      421 ataccgccac gattggagcc gccctgcgtc gacggtgaag ggacccatga ctgattgttg
      481 taccgcaccg gcacggcggt ttcatatgag gtccagaagg ccaccggtgt ggcatccccg
      541 cctttggggc cctccagctc aaagtcgccg ttgggcagca actggctgcc gggaggcgag
      601 tagcggaacg ccaggggcag cggggcgggg ctgtcacact gatcaccgcg aacggccacc
      661 aggtcgtaga agcccgccgg cgccgaagca aaatcgaatc tcgccaccac gtcgctgccg
      721 ctgggaacca cattcgtcgc gaccagctcg gtcgccccct gcacgagttt gatgctgttg
      781 gccgagtcgg ccaagacggt cacattcgta ccggtgatct tcaattccgt ctgcccctcg
      841 cggaccacga tcgcgctcgg cgacacgccg gttagtgtca ccgggtcgac gcagaacagg
      901 tcaaaagcgt ccacgaaagt ccgtggcacg ttgtcgtgga atcgcagcga accggcgccg
      961 gtgcttcgac gcacgcgata gaactcgagg ttcgccggcg gagcgttcgg cttctcaaga
     1021 acgaggttgt agaggtcggc atgagcaccg ctggtatgca tcgggaagct cacgatcaac
     1081 ttggtcggac tgtgggtgag aatcgtcccc ggcaccaccg tctccgcctg atctccggaa
     1141 gccttgcggt caacgaagac gagcttgacc gcgcttacct cgtccagatt cgatcccccg
     1201 gtgatcacga gggtgggatc cacgtttgtc ggtcccctca ccggataagc gatccacgga
     1261 tgcaacggac ccgtgcaggc cggcgaggcg gtcaggctga atgcgtcggc aaatgcgccg
     1321 gccgcgctgt accccgggca gaccaattcc gtcgccagtt ccaccgtcac atagccggaa
     1381 tacgacgtcc cgttgatcgt caccccgacc caggtgccat aatccccgcc ggctccggcc
     1441 gtcgtgtggt cgatgcttcg actgccgatg gttgagccgg tcttgtcgcc gtcatacagg
     1501 gtgaccttcg ctttggcctg agacgtgccg aagcgattgc agtccagcca accgctgaat
     1561 gacacggccg agcccggggc tatcggcacc gtttgccaca tggatgtcgt ggaagtgccg
     1621 ggatgccccc gactcgtgga ccagataccg taaatgccaa aatagtccgt ccggtagtcg
     1681 atggaagtcg ggttggggtt cgcggccggg ttcttgacgt aatctccgaa ccacatggcc
     1741 gcattgccca cggcatccat gggagcaagc ggtttcccgt ccgagccgac gttcaccacc
     1801 tcgaagctcg ggttcgccag gaggttctgt ggcacgctcg gatcaaggat ctcgaaggcg
     1861 ttggagatga ccaccggatt gacgcccgcc tgctcgacca ccaagtcgaa cacgcctgcc
     1921 gcttgccccg ctgtcgcaag actgaccgtc agggacgtgg cggtttgcgc agtaatcgtg
     1981 ccggcaatta acacgccgtt ggctccgttg ttgttccaca ttcccacctg tgttttccag
     2041 agatgcactc cggtcacatt cgtgaggttg gtgccggtga tcgttaccgt cgtatcggtg
     2101 gccaccggcg tgctgatcac gttcggctcg atgtccgtga catcgtgctg gccggcacaa
     2161 gtgccttcgc tgcatgcaaa caactccaca tcatccagcg cggcaatgcc ccacccggtt
     2221 ccccccgcgc cgtccccgac cagcgtgaac cgcaccttca cccagccgct ggccacctcg
     2281 cccgcccagg tagcccatac ctgctggtat gattgcgagg gcggaacgcc gtccgtgctg
     2341 ctcttgaccg ctgcggccac cacgacgtcg tccacgagaa gctcgcctgc cacaacgctt
     2401 ggccaagccc ccgtgtcata cacccacgcc caaaacgaca gcgccaggtt atagtacccg
     2461 ggcacgacgg aaacggtctg ctcaagaacg ccgatatcac agttgtaact gctgggattc
     2521 atgacaaagc gattacccgt gcgtgcgctt gctccgtagg tcggggcgct gcagtaatcc
     2581 ac
//
LOCUS       DAOTPW010000409         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_31418_length_2569_cov_3.807080, whole genome shotgun sequence.
ACCESSION   DAOTPW010000409 DAOTPW010000000
VERSION     DAOTPW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31418_length_2569_cov_3.807080"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="P5316_21570"
     CDS             <1..1829
                     /locus_tag="P5316_21570"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRR87604.1"
                     /translation="LHPSDTQPAEQATVGLVPPVSQVQNGSGRLVVTGRVTILLPAVD
                     DAVTSYAARCLGEELNRLFGLQTVTVRQPHRISDDEICAIISDQSSSSSPLENMFCSL
                     PVPKADGYLLGVRPDEKRAVVHGFGGNGVIRGVFALAGLLSADGGEATWPEVLISDTP
                     DIPIRFTRDIFLNKQATAEMTQEQALICELDWWARWGLNHALIPSGLTKGQEEQDRLV
                     RWYVEEAHRRGMKAGANPGGRSLCPSNPAEMESYLSRVRHLLGLGCDFLLILFDDLPR
                     ARLGGHCDRCVRAFGGSLAAEQRHILESVCNLLPTTRSDLPLFWCPTYYSLGMTGYIG
                     GPEGPDEYFTDLGGSARVCRAFMFHCAFDHEFNAYLDGKGLKNRIWWYNGIRTDYYMV
                     SRAFDSCDMWGPPLRIPGLKDFQSFFSRFENGWLMPSFAPTDRSLHPCVAPLVTAGRD
                     EGGRTIIPKASLDELAYLGERMQGLYLCSATAPYHIALAGVFAAHPSRFDQQLARAAI
                     ADAMFSPGASPHVLAWQTAYARAQMVLARKQGCPLTPEGNAEIIALTGEMESGEKALR
                     DCLARRKSALPASVAAALLDEMIAWRLKVLSLAAPYPPARPP"
     gene            1921..>2569
                     /locus_tag="P5316_21575"
     CDS             1921..>2569
                     /locus_tag="P5316_21575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR87605.1"
                     /translation="MIAVAARLRCPVQLFSSGCVRLTGTTILLIAASCAGPSSVADRG
                     PFTIVMLPDTQRYSESRPDLFFVQTDWIRKNRDKENIVFVTHVGDLVQNRSKKPSEWK
                     TADEAMAVLDGVVPYGVAIGNHDYDSDDGVKKGVATMYLQYFNPEQRFKGRPGYGGAS
                     PNGLNSYHLLSAGGIDLIMLFLEVEAPDDALAWASSVLEKYPTCAAIVSIHTYMKH"
BASE COUNT          485 a          806 c          752 g          526 t
ORIGIN      
        1 ctttgcaccc gtccgacact caaccagccg agcaggcaac cgtcggcctg gttccgccgg
       61 ttagccaggt gcaaaacggt tcaggccgcc tggtcgtcac aggtcgggtg acaatccttc
      121 tccccgccgt cgacgatgcc gtcaccagtt acgcggcaag gtgccttggt gaagagctta
      181 atcgcctctt cggcctgcaa acggttaccg ttcgccagcc acatcgtatc tcggatgacg
      241 agatctgcgc gatcatcagc gatcaatcct cctcgtcatc gcctcttgaa aacatgtttt
      301 gcagtctccc tgtgccgaag gcggacggct atctgctggg ggtgagaccg gacgagaaaa
      361 gagccgttgt ccacggattc ggtggtaacg gggtcattcg cggcgtcttc gcactggccg
      421 gactgctgtc cgccgacggc ggcgaggcga cctggcccga agtgctcatc tctgacactc
      481 ccgacattcc aatccggttc acacgagata tattcttgaa caagcaggcg accgcggaga
      541 tgacacagga gcaggcgctg atctgcgaac tggactggtg ggccagatgg ggcctgaatc
      601 acgcattgat cccctccgga ctaaccaagg gccaggaaga acaggatcgg ctcgttcgat
      661 ggtatgtcga ggaggcccat cgacgaggca tgaaagcagg ggccaacccg ggtggtcgct
      721 ccctctgccc tagcaacccg gcagaaatgg agtcgtatct atcgcgggtg cgtcatctcc
      781 tgggcctggg ctgcgacttc ctcctgatcc ttttcgatga ccttccacgc gctcgccttg
      841 gcggccattg tgatcgctgc gtccgggcct ttggtggaag cctcgccgcc gagcagcgcc
      901 atattctgga atcggtgtgc aatctccttc cgaccacccg atctgatctc ccgctcttct
      961 ggtgccctac ctactacagc ctggggatga ccggctacat tggggggccg gaaggaccgg
     1021 acgagtactt cacggacctg ggcggatccg ctcgcgtttg ccgggccttc atgttccact
     1081 gcgcgtttga tcatgaattc aacgcttatc tggacggcaa gggactcaag aaccggatct
     1141 ggtggtacaa cggcatcaga accgattact acatggtcag ccgggcattc gacagttgcg
     1201 acatgtgggg gccgccgctc cgcatcccgg gcttaaagga tttccaaagc ttcttttcgc
     1261 gttttgagaa cggctggctc atgccctcgt ttgcccccac cgatcgttcg ctgcatccct
     1321 gcgttgcgcc gcttgtgacc gccggtcgcg acgaaggcgg gcgaacaata atcccaaagg
     1381 cctctctgga cgaactggcc tatcttggcg agcggatgca gggattgtac ctatgcagcg
     1441 ccaccgcgcc gtaccacatc gcccttgccg gggtttttgc agcccaccca tcccgtttcg
     1501 accagcagct cgcccgggca gccatcgccg atgcgatgtt ttcgcccgga gcgtcaccgc
     1561 atgtgctcgc gtggcagacc gcttatgcgc gggctcagat ggttctggct cggaaacagg
     1621 ggtgtccgct gacccccgaa ggaaatgcgg aaatcattgc cttgaccggc gagatggaat
     1681 ccggcgagaa ggccctccgg gattgcctcg cccgacggaa atcggctctg cctgcctcgg
     1741 tggctgctgc attgctggat gagatgatcg cttggcggct caaggtgctc tcgctcgccg
     1801 ccccctatcc tccagcccgg ccgccttgag gcgccgccgt ggtcatgggt cggcctcgtc
     1861 tggctgacgt cagggatggc ctccaataat atccaatgac cattgagaaa gggaaacaac
     1921 atgatcgctg tcgccgccag acttcgttgt cctgttcagc ttttctcatc cggctgtgtt
     1981 cggctcacag gcacgacgat cctgctcatc gctgcttcat gcgccggtcc atcgtccgtt
     2041 gccgaccgag gccccttcac gatcgtcatg cttccggaca cgcagcgcta cagcgagagc
     2101 cgccccgatc tgtttttcgt ccagactgac tggatcagga agaaccgtga taaggagaac
     2161 atcgtcttcg tgacacacgt gggcgatctg gttcagaacc gcagcaagaa gccgtcagaa
     2221 tggaagaccg ccgacgaggc gatggctgtc ctcgatggcg tcgtgccgta tggtgtggcc
     2281 atcggcaacc acgactacga cagcgacgat ggcgtgaaaa agggcgtcgc caccatgtac
     2341 ttgcagtact tcaatccaga gcaacggttc aaaggccggc cgggttacgg cggcgcctca
     2401 ccgaacggcc tgaattcgta tcacctactg tcggccggcg ggatcgacct gatcatgctt
     2461 ttcctcgagg tcgaggcgcc tgatgacgca ctggcatggg caagcagcgt gctcgagaag
     2521 tatcccacgt gcgccgccat cgtcagcatc cacacctaca tgaaacaca
//
LOCUS       DAOTPW010000410         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_32755_length_2489_cov_4.405094, whole genome shotgun sequence.
ACCESSION   DAOTPW010000410 DAOTPW010000000
VERSION     DAOTPW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32755_length_2489_cov_4.405094"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="P5316_21580"
     CDS             <1..1583
                     /locus_tag="P5316_21580"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HRR87606.1"
                     /translation="AHNDWQDQHPDQPGDPTIPEFDRDRYTETCAGCHARRGTLTGNF
                     IPGEKFLDHYDPVIPDLSDIFYPDGQVREEDFEYVPFSLSLMYAWGVRCIDCHYYHGG
                     KVATTENRLCLRCHEKGIATKRPIDEVEHSRHPSDKPGFKCVDCHMPQTVYMARHWRR
                     DHGMTIPDPMLNKEHGIPDACMRCHEKEGLDWNIKYVREWYGDRMDRPTQRRARLQAR
                     LKAGDPSAAPDLLAMLREEKNANWRAVNLKFLDAVLQSPDPSPLRQAVAIEFVSRLGD
                     ESPLVQAVVIDALEPLGQSVTQLLAPMLASPSRLVRIKAAWALRRQIDLTSSPGRELT
                     AFIEYRCDQPMGAYQQARLLMDTGRSAQAAPWFERALKWDSRLAPARHEYATLLQQQG
                     RSFDAVKVLHDGTRIEPNNPYYPYWLGLLYSEMGENLAARDSLRAAVQRDASQARFWY
                     NLSLAEHKTGGTQEALDAIARAEQLSPRAPFYPYTRATIQMESGQSDLARQALNRCLE
                     IDPQYRPALEMLQAMANQ"
     gene            complement(1686..>2489)
                     /locus_tag="P5316_21585"
     CDS             complement(1686..>2489)
                     /locus_tag="P5316_21585"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRR87607.1"
                     /translation="DQRLRVEMANRNDCDVFVSIHHNANDANPNANFVSVWYHDDPDH
                     SPASLDVARQLAVALMDELRLPQQLGCPIMSDKLIYAKSGFAVLRQAKVPAVLCECSF
                     HSNPEEESRLRNPEYNRREAHALFVGLARYAYAGIPRVQLLDPPDGLLPESGAKAILI
                     ELNDGIRDRKSWGWDRPLILSDSIVVRQDGKDLPFTFDKQTNLLTVPLPSRLKLGSIR
                     LEVRLMNLFKQSNPKTSLELKVVKKLPPNRPVARAATAPVPAPATEESE"
BASE COUNT          478 a          839 c          720 g          452 t
ORIGIN      
        1 aggcccacaa cgactggcag gaccagcatc ccgaccagcc cggcgacccc accatccccg
       61 agttcgaccg cgaccggtac accgaaacct gcgccggctg tcacgcccgc cgcggtacat
      121 tgaccggcaa cttcatcccg ggcgagaagt tcctcgacca ctatgacccg gtgattcccg
      181 acctttctga cattttctat cccgacggcc aggtccgtga ggaggatttc gaatatgtcc
      241 ccttctcact cagcctgatg tacgcttggg gcgtgcggtg catcgactgc cactactatc
      301 acggcggcaa ggtcgccacc accgagaaca gactgtgcct tcgctgccac gagaagggca
      361 tcgccaccaa gcggccgatc gacgaggtcg agcatagccg ccacccgtcc gacaagcccg
      421 gcttcaagtg cgtcgattgc cacatgccgc agacggtcta catggcccgt cattggcgcc
      481 gcgaccacgg catgaccatc cccgatccga tgctcaacaa agagcacggc atccccgacg
      541 cttgcatgcg ctgccacgag aaggagggcc ttgactggaa catcaagtac gtccgggagt
      601 ggtacggcga ccggatggac cgtccgacac aaaggcgagc gcggctccag gcccggctca
      661 aggccggtga tccgtccgcc gcccccgacc tgctggccat gcttcgcgag gagaaaaacg
      721 ccaactggcg ggcggtgaac ctcaaattcc tcgatgcggt gctccagagc ccggacccat
      781 cgccgctgcg gcaagctgtg gcgattgagt tcgtcagtcg gctgggggat gaatcgccgc
      841 tggtccaagc cgtggtcata gacgcccttg agccgttggg tcagagcgtc acccagctat
      901 tggctccgat gctggccagc ccaagccgtc tggtgcgtat caaagccgcg tgggccttgc
      961 gaaggcagat cgatctgacc tcgtcgcctg gccgcgagct gaccgcgttt atcgagtatc
     1021 gttgcgacca gcccatggga gcctaccagc aggctcggct cctgatggac accggacgtt
     1081 ccgctcaagc ggctccgtgg tttgagcgag ccctcaagtg ggattcacga ctggctcccg
     1141 cccgccacga gtatgccacg ctcctgcaac agcaggggcg gtccttcgat gcggtcaagg
     1201 tgctgcacga cggcacgcgg atagaaccta ataaccccta ttacccctat tggctgggtc
     1261 tgctctatag cgagatggga gaaaacctcg ccgcccgcga ttcgctccgg gccgccgtcc
     1321 agcgcgatgc ctcgcaggcc cgtttctggt acaacctatc actggccgag cataagaccg
     1381 gcggcaccca agaggccctt gacgccatcg cccgcgccga acagctttcg ccgcgagcac
     1441 cgttctaccc ctacacgcgg gctaccatcc aaatggagtc gggccaatcc gaccttgccc
     1501 gccaggccct caaccgctgc ctcgaaatcg acccccaata ccgcccggcc ctggaaatgc
     1561 tccaggcaat ggcgaaccag tgaacgcctc gcggatgccg ctgttgcgct caggcctcca
     1621 tcaatcctcc tgacagtcta gatgactaat tgactaacag accaatagac ccacagactg
     1681 ctgccctact cgctctcctc cgtggccggt gcgggcaccg gggccgttgc ggccctcgcg
     1741 accggtcggt tcggtggaag cttcttgacg accttcagct ccagcgacgt tttcgggttc
     1801 gactgcttga acaggttcat caatcggacc tccaagcgaa tcgatccgag cttgagcctg
     1861 ctcggcaggg gtaccgtaag caggttggtc tgcttgtcga aggtgaacgg caggtctttt
     1921 ccatcttgtc gcacgacgat gctgtcgctc aggatcagcg gccggtccca tccccagctt
     1981 ttgcggtcgc ggatgccgtc gttcagctca atcaggattg ccttcgcacc gctctccggc
     2041 aaaagcccgt ctggcgggtc caggagctgc acgcgaggaa tccccgcgta ggcgtaccgg
     2101 gccagcccga caaacaaggc gtgagcctcg cggcggttgt actcggggtt tcgcagccgc
     2161 gattcctctt ccggatttga gtgaaacgag cactcgcaaa gaactgccgg caccttcgcc
     2221 tgccgcagca cggcaaaccc gctcttggca tagatcagct tgtcactcat gattgggcaa
     2281 ccaagctgct gaggaagtcg cagttcatcc ataagcgcca ccgccaactg ccgggcgacg
     2341 tccaggctgg ccgggctgtg atcgggatcg tcgtggtacc agaccgaaac gaaattcgcg
     2401 ttcgggttgg catcgttggc gttgtgatga atcgacacga atacatcgca gtcgttgcga
     2461 ttggccatct cgacccgaag ccgctggtc
//
LOCUS       DAOTPW010000411         2459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_33316_length_2459_cov_5.184692, whole genome shotgun sequence.
ACCESSION   DAOTPW010000411 DAOTPW010000000
VERSION     DAOTPW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33316_length_2459_cov_5.184692"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..443)
                     /locus_tag="P5316_21590"
     CDS             complement(6..443)
                     /locus_tag="P5316_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87608.1"
                     /translation="MSRQDDQKGQQRSRDPQIIREDLLRPSHYLGYDRDDLGCYFLGR
                     EAFELAESQFRRAVWLNPYEPVFKIHWAVALTHLNRMREAHDLLVGVFRNRQDNALAR
                     QLWHHFWPAEPLPVPEPASSDLGKVDPATQDPAREAECRDPPC"
     regulatory      complement(552..615)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(702..1268)
                     /locus_tag="P5316_21595"
     CDS             complement(702..1268)
                     /locus_tag="P5316_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87609.1"
                     /translation="MLQLVEMLNRFYKWAEQFHDQGEQRFYGVLIRLVRQFYGHTSAA
                     EFGPRKLRFLRYQMVRGDENAKPPRRPRSRKYINSQVQRIRRMFKWAASQELVPVGVC
                     RSLRIIESLRRGRSGARENAKVGLVEQRLLDVTLPRLSRPVKALVDLWILTGARPNEL
                     PGLRGCDLEMDNETGIRLYRPGKHWTIC"
     gene            complement(1624..1696)
                     /locus_tag="P5316_21600"
     tRNA            complement(1624..1696)
                     /locus_tag="P5316_21600"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1661..1663),aa:Thr,seq:tgt)
BASE COUNT          468 a          803 c          714 g          474 t
ORIGIN      
        1 ttcatctaac acggcggatc gcggcattcc gcctcacggg caggatcttg tgtggcagga
       61 tccaccttcc ccaggtccga cgaagccggc tccggtacag ggagtggttc agccggccag
      121 aagtggtgcc acaactggcg ggccagagca ttgtcctggc gattcctgaa gactccgacc
      181 agcaggtcat gagcctccct catgcgattg agatgggtta aggcaacggc ccaatggatc
      241 ttgaacaccg gctcataggg attgagccag acggcccggc ggaattgtga ctccgcaagc
      301 tcaaaagctt cccgtccgag gaaatagcag cccagatcat cccggtcgta gcccagatag
      361 tggctgggcc gcaacaagtc ctcgcggatg atctgcggat cgcgacttcg ctgttgccct
      421 ttctggtcgt cctgccggct catggtccgc cgcgcctctt cgccgcaggc ccgtgcctgc
      481 caacaaaaaa ccccgcacag gaatcgtcct ggcagggctc cgttggcgcc gtttcgcccc
      541 tacccaggtc gattcgggta ggagtcatca ggtcggcagg accgcttgaa ggcggactcc
      601 atcgccacac gtatcttaac cccgccgggc gcactccgca agggctattc actgttgacc
      661 gtttgggcac caggtccgaa gtggatgatc tgccccttct ctcagcagat ggtccagtgc
      721 ttcccgggac gatacagccg gatgccggtt tcgttatcca tctccaaatc gcaaccacgc
      781 aacccaggca attcgttggg tcgtgccccg gtcagaatcc acagatcaac aagggctttg
      841 accggccggc tgagacgagg cagcgtcacg tcgagcaggc gttgttcaac gaggccaacc
      901 ttcgcgttct ctctcgctcc tgatctgccg cgccgcaatg actcgattat acgtagcgat
      961 cggcagaccc cgacaggtac gagttcttgc gatgccgccc atttgaacat gcggcggatt
     1021 cgctgaacct gggaattgat gtacttgcga gaccggggcc gccggggcgg cttggcgttc
     1081 tcgtcaccac ggaccatctg ataccgaagg aatcgcagct ttctggggcc gaactccgca
     1141 gcggaggtgt ggccatagaa ctgacggacg agacgaatca gcacgccgta gaagcgttgt
     1201 tcgccctggt cgtgaaactg ctcggcccac ttgtagaacc gattgagcat ctcgaccagt
     1261 tgcaacaccc cgggggcgcg gggggggcat ctctccagga taccgctctg gccggcggcg
     1321 gcccttggcc tcccgctcgg cgatcacgcg gtggtacagc tcaccgctct gcggcgagcc
     1381 atgttcgcga gccggcggtc gcgacgtttc ctcatggcga agtcggtgag ggtaaagagg
     1441 gcttgtgaat ggccctctcg ccggcggtac caaggagcct tcgggttggc gcttttctgt
     1501 cggcatacgc ggacctccct gacgccggca cggtcagtga ccgcgctaaa acgcgcttca
     1561 ggccgcactg ccgaacgccg acagatcctc gtaacccccg acggcgacaa gacctatcgt
     1621 gacagccacc ggtcggactc gaaccgacga cctgcggttt acaaaaccgc tgctctaccg
     1681 actgagctac ggtggcaaca atgccggtgc aacaaatcac tgcaaaaact cgcccgagcg
     1741 gataccttcg ctacagcctg tgggtcccac cttcggccaa agccatgctc ggggtgtcaa
     1801 tcatggctcg ttctcgcaag ccagacctcc ggccccgcaa ggcgatcggg gcacatttgc
     1861 acgaacccgt cggacggcac ttcaagtgcc tctccaagga tccggaaaca gctcatggcg
     1921 agcatgcctg gcggctcctc gccccgcagt ttccagcctc gcagaaccac gacctgatta
     1981 taccgcttcg gacgccggtg caaagcagcc tgtggtggtc tgaaatgtct gcaacgcctg
     2041 cctgagcttt gtccagagga acgacgcctc gaacatttca agtgctatgg tgaaggccgg
     2101 acgtcgcctt tgcggccata gacgcaagaa atcaccaagg cggccttcgg ccgtagccca
     2161 aacatgtgcg cgacttgctc gcgtgaggcg acgcaagtat ccgaatgcgg gtgtcccgcc
     2221 ctgcggcttg cctctctggc gtatcgccct tcgcccataa cccatgcatc tgccgcataa
     2281 ggccggccct tccaaagcga cctcttcttc tcccgacggc taacgccata gtcatccggt
     2341 actttagcct ctcccgtcgc gactggtgcg cattttgcgc gcatgtttct cgatcgcagg
     2401 gcgccgggtg ccatgcccac ggctttgcgt gggcatgcgc ctccaaatgt atccgctcg
//
LOCUS       DAOTPW010000412         2438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_33722_length_2438_cov_4.617289, whole genome shotgun sequence.
ACCESSION   DAOTPW010000412 DAOTPW010000000
VERSION     DAOTPW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33722_length_2438_cov_4.617289"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2438)
                     /locus_tag="P5316_21605"
     CDS             complement(<1..>2438)
                     /locus_tag="P5316_21605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HRR87610.1"
                     /translation="VTDREEFRHAGKKVFGKAQTRMELIARTETMRAHSQGQRKFYAT
                     VGVQKLEWLTMQDERMCPECAALDGKVYPVDQFPNQPLHPACRCGHVAVVDMPICGAG
                     SLASEAADAPGCILSPQDVEAEAEAVKADVQAVNTALDTGSFESLTVKQLQTAAKKQG
                     IAIARTKSDFLKLLDQAEPGVDHSALAGQALKDKLAQYQIGALRTKQELTDLLKAKHK
                     AAVQEESLQAMTPPAGGYDQFTVKQLQEMALQKGVSLHMTKQDVIDYLDKVEPGVDHS
                     TLSGPSLVQAKEKFGVGALKNKQQLVDALNKSAGQEAAEQAKKQTEQAELAAKVAQAQ
                     TDLAAKTAAVQIPDDPMQYGKFLESVKAAENQVAQSGIVPKDVLDAHAKEVALKKQVF
                     QDKIAVLPNADVKKLAQKTQIKHYQWASKGELVTLLTETDAAKVQAAKDGIEAKWAKW
                     AEKHGSKKTKTSEGPPPKPAPAPPPAPAPKAQPPSAPPTQAPVFAKKGSEFETADAAW
                     QEKGQPTNFKFQGKADIEGAHTKYFYTDEKGEKWLFKPVPEEFRAHGDEVAYRIGRLI
                     DPEAIEVRVIALDGRVGSIQQWRTGLAKQKDFAGLDPNDLSPRDIEQLQREHVIDWLI
                     SNHDGHGKQFLRMQDGHVYGIDKGQLFKFLGDDRLSLDYHPNQAHGESEPYYNTLFRA
                     VKDGKVKADPAVTLRYVREVEKISDADYRAILAPYAERRFGDKAAQKEAFYQAALERK
                     NNIRLDFEKFYGDVLGQKDFRFEAAEPEPSKVGRLTKTEVGILAEVRKAGWQGKVIPF
                     DVEDVEDQNALVFV"
BASE COUNT          332 a          795 c          754 g          557 t
ORIGIN      
        1 cgacgaagac cagggcattc tggtcttcca cgtcttcgac gtcaaacgga atcaccttcc
       61 cttgccagcc ggccttgcgg acctcggcaa ggatgcccac ctcggtcttg gtcagccggc
      121 caaccttgga cggttccggc tctgcagcct cgaagcggaa gtccttctgt ccaagaacgt
      181 ccccgtagaa cttctcaaag tcaaggcgga tgttgttctt gcgctcaaga gccgcctgat
      241 agaatgcttc tttctgcgct gccttgtcgc cgaacctccg ctcggcatac ggggccagga
      301 tcgcgcggta gtcggcgtcg gagatcttct cgacctcgcg aacgtaccgc agcgttacgg
      361 cggggtcggc cttcactttg ccgtccttca cggcgcggaa gagggtgttg tagtacggtt
      421 cgctctcccc gtgggcctga ttggggtggt agtcgagact gagccggtcg tcgcccagga
      481 acttgaacag ctgtcccttg tcgatgccgt agacgtggcc gtcctgcatc cgcaggaact
      541 gcttgccgtg cccgtcgtgg ttgctgatca gccaatcgat gacatgttcg cgctgcagct
      601 gctcgatatc gcgcggcgag aggtcgttcg gatcgaggcc cgcgaagtcc ttttgtttgg
      661 ccaggcccgt cctccattgc tggatggagc cgacgcggcc gtccagtgcg atcacgcgca
      721 cctcgatggc ttcgggatca atgagcctgc cgatccgata agcgacctcg tcgccgtgcg
      781 cccggaactc ctccgggacc ggtttgaaca gccacttctc gcccttctcg tcggtgtaga
      841 agtacttggt gtgcgcgcct tcgatgtccg ctttgccctg gaacttgaaa ttcgtgggct
      901 ggcccttctc ctgccacgcc gcgtcggccg tctcgaattc cgatcctttc ttggcgaaga
      961 ccggagcctg agtcgggggg gccgaagggg gctgtgcctt gggcgcgggc gctggaggcg
     1021 gcgcgggagc gggcttgggc ggcggtccct cgctcgtctt ggtcttcttt gaaccgtgct
     1081 tttccgccca cttcgcccac ttggcctcga tcccgtcttt ggctgcttgg accttcgccg
     1141 catcggtctc cgtgagcagc gttaccagtt cacccttgct cgcccactgg tagtgcttga
     1201 tctgggtctt ctgcgccagt ttcttcacgt cggcgttggg caggacggcg atcttgtcct
     1261 ggaagacctg cttcttgagc gcgacttcct tcgcgtgggc gtcgagcacg tccttcggaa
     1321 cgattcccga ctgggcgacc tggttttcgg ctgctttcac cgactccaga aacttcccgt
     1381 attgcatcgg gtcatcggga atctggacgg cggcggtttt cgcggcaagg tcagtctgcg
     1441 cctgcgcgac cttggcggcc agttcggcct gctcggtctg cttctttgcc tgctcagcgg
     1501 cctcctgccc ggcgctcttg ttcagcgcgt ccaccaactg ctgcttgttc ttcagcgcgc
     1561 cgacgccgaa cttctccttc gcctggacca gcgacggccc tgacagcgtc gagtggtcca
     1621 cgcccggctc gaccttgtcg aggtagtcga tgacgtcctg cttcgtcata tgcagggaga
     1681 cgcctttctg gagggccatc tcctgcagtt gcttgaccgt gaactggtcg tacccgcccg
     1741 ccggcggcgt catcgcttga agggactcct cctggaccgc cgccttgtgc ttggccttga
     1801 gcaggtcggt cagttcctgc ttcgtgcgca acgcgccgat ctggtattgc gcgagcttgt
     1861 ccttgagcgc ctgacctgcc agcgcagagt ggtccacgcc cggttcggcc tggtcgagca
     1921 gcttgagaaa gtcggacttc gtgcgcgcga tggcgatgcc ctgctttttg gccgccgtct
     1981 ggagttgctt caccgtgagc gactcgaagc tccccgtatc cagagccgtg ttcacggctt
     2041 ggacatcggc cttgaccgct tcggcttccg cctcgacgtc ctgtggggag agaatgcagc
     2101 cgggcgcatc ggccgcttcc gacgccagcg aaccggcccc gcagatcggc atgtccacca
     2161 cggcaacgtg tccgcagcgg caggcgggat gcagcggctg attcgggaac tggtctacgg
     2221 ggtagacctt gccgtcaagc gccgcgcatt ccgggcacat gcgctcgtcc tgcatcgtca
     2281 gccattcgag cttctgtacg ccgacggttg catagaactt ccgctgaccc tggctgtgcg
     2341 cgcgcatggt ctccgtgcgt gcgatcagtt ccatgcgggt ctgggccttg ccgaagacct
     2401 tcttgccggc gtgccgaaac tcctcccggt ccgtcacg
//
LOCUS       DAOTPW010000413         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_34451_length_2399_cov_5.406997, whole genome shotgun sequence.
ACCESSION   DAOTPW010000413 DAOTPW010000000
VERSION     DAOTPW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34451_length_2399_cov_5.406997"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="P5316_21610"
     CDS             <1..650
                     /locus_tag="P5316_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRR87611.1"
                     /translation="RNTHFSYFMGVDGVEQFVSTPELHSDEAIEADPLPPGQVWAISP
                     GSAESAPALYRIEATCGPGSGVKILNAPVPPAFRESVRCGEQNLYSRAKELVGDRDPR
                     NHEFTVQLRAMDTDRSGHGLGLPVLLSLCGSLMERSTKGGLIVVGSLNLGGSVEMIPN
                     PVAVAELAVEKGAAQVLMPISARRQLLDLPDEMATKVNIVFYADPADAFMKAILE"
     gene            700..954
                     /locus_tag="P5316_21615"
     CDS             700..954
                     /locus_tag="P5316_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87612.1"
                     /translation="MASFRLDRFFSQPEILRGIDPENLLSLLADHAKALETLSSKLPD
                     VADAAATIDYDALTSLLMEPDKLPTGLREAFHFIHEMATP"
     gene            958..2007
                     /locus_tag="P5316_21620"
     CDS             958..2007
                     /locus_tag="P5316_21620"
                     /inference="COORDINATES: protein motif:HMM:NF024800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HRR87613.1"
                     /translation="MECLLAAAEEAGIEIVGTPGPTPGDVAVQVWLKDRELLERKHAE
                     QFLRNPRTFESFGTAVSPVPKYRDPMKLTDAMKKDLDDDETFAEAQNRLALHARPRRD
                     IGQLLAPAYRPFTGMIFCEECGSVCYRRTSKNRKGEYNYYGCGCRQRNGPGACDNGAS
                     VREDLLMERIKRTYEEVFADADSIIEDAIEEARKLMQSNCGELQRVRGQIGELDKKIG
                     SMTRLLVDPDIEATAKRAVSRQIGELEAERERLQNAVAELASDANDNTARLASAVRQA
                     LGEAQESLATVTTPTEIRDFIEQYVGPMMLKRNGEIERKGLEILTETQTAPAEAEAVK
                     RSIAGVGLEPTTSGL"
BASE COUNT          502 a          710 c          794 g          393 t
ORIGIN      
        1 tccgcaacac gcacttcagc tacttcatgg gtgtggacgg cgtggagcag ttcgtatcga
       61 cgccggagtt gcacagcgac gaagccatcg aggccgaccc gctgccgccc ggacaggtct
      121 gggccatcag ccccggcagt gccgagtcag ccccagcact ctaccgcatc gaggcgacat
      181 gcgggccggg cagtggcgtg aagattctca acgcgccggt gccgcctgcg ttccgtgaga
      241 gcgtccggtg cggcgagcag aacctgtact cgcgggccaa ggaactggtg ggcgaccgtg
      301 atccccgaaa ccacgagttc acggtgcaac tgagggcgat ggatacggat cgatccggcc
      361 acggcctggg cctgccagta ctgctgtcgc tgtgcggaag cttgatggaa cggagcacaa
      421 agggcgggct cattgttgtc ggctcgctca acttgggtgg ctcggtggag atgatcccca
      481 accccgtggc tgtcgccgag ttggccgtgg agaaaggtgc agctcaagtt ctcatgccca
      541 tatcggcgcg aagacaacta ctggacctgc cggacgaaat ggcgacgaag gtaaacattg
      601 tgttctacgc cgacccggcg gatgccttca tgaaagcgat actcgaatga gtttgcattc
      661 ggcagggacg ccggtgcgcg tccaaggaac gtaacgacta tggcgagctt tcggttagac
      721 cggtttttct cacagcctga aatcctccgg gggatcgacc cggagaacct gctttcgctt
      781 ctcgcggacc acgcgaaggc gctggagacg ctgagctcca agctgccgga cgtggccgat
      841 gcagcggcca caatcgacta tgacgcgctg acctcgctgc tgatggagcc ggacaagttg
      901 ccgacgggct tacgagaggc cttccacttc atccatgaga tggccacgcc gtgagggatg
      961 gagtgtctgc tcgcagccgc tgaggaagcc ggcatcgaga tcgtggggac accaggcccg
     1021 acgcctggcg acgtggccgt ccaggtgtgg ttgaaagacc gcgagcttct ggagcgcaag
     1081 cacgccgagc agttccttcg aaacccacgg acctttgagt ccttcgggac cgccgtgtcg
     1141 cccgtgccga agtaccgcga cccgatgaag ctgaccgacg cgatgaagaa ggacctggac
     1201 gacgacgaaa ccttcgccga agcacagaac cggctggctc tgcacgcccg gccccggcgg
     1261 gacatcggcc agcttctcgc cccggcctac cggccgttca ccgggatgat cttctgcgaa
     1321 gagtgcggca gcgtgtgcta tcggcgaacc agcaagaacc gcaagggcga gtacaactac
     1381 tacgggtgcg gttgccgcca gcggaacggc ccgggcgcct gcgacaacgg ggcgagcgtc
     1441 cgcgaagacc tgctgatgga gcggatcaag cggacctacg aagaggtctt cgccgacgcc
     1501 gactcgatta tcgaagacgc catcgaggaa gcccggaagt tgatgcagtc caactgcggc
     1561 gaactccagc gggtccgcgg ccagatcggg gaactcgaca agaagatcgg ctcgatgacc
     1621 cggctcctgg tggacccgga catcgaggcc acggccaagc gggcggtatc gcggcagata
     1681 ggcgagttgg aagccgagcg cgaacggctc cagaacgccg tggcggaact ggccagcgac
     1741 gccaacgaca acaccgcccg gctggccagc gcggtccggc aagctcttgg tgaagcgcaa
     1801 gaatcgcttg cgacggtgac gacgccgacg gagatacggg acttcatcga gcagtacgtt
     1861 ggcccgatga tgctcaagcg caacggggag atcgagcgga agggcttgga aatactgacc
     1921 gaaacgcaaa cggccccggc agaagccgag gccgtgaagc ggtcaatagc gggggtagga
     1981 ctcgaaccta cgacctccgg gttatgagca cggcgagcca tggaaagcgt aagtcgtgct
     2041 ctgcacgtaa cctacagaat tcacagtaag ttgcatcgtt ttgtcgttac tccacaagtg
     2101 ctacatgtgc aacatgtaga gcaacggatt tggctcaatt ctagccaccc cctgtggctg
     2161 gcgcaagagg tatcccgaag cacatggtaa aggcgcgcgg gaactgagcc gtggagcggg
     2221 taggaagtga gtttggacga gcacggccgg tcagcggccg gttgctgggg cgccagcggg
     2281 gggccaaaat gtcggaggct gtacattgct gctgaaaaca cgctcttcat tcaggattta
     2341 ctcgccatgt tgcggtggtt ctacgtcctc gctcacctcc ttgtcgaggc aggcgccgc
//
LOCUS       DAOTPW010000414         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_34798_length_2381_cov_4.768702, whole genome shotgun sequence.
ACCESSION   DAOTPW010000414 DAOTPW010000000
VERSION     DAOTPW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34798_length_2381_cov_4.768702"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="P5316_21625"
     CDS             <1..212
                     /locus_tag="P5316_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87614.1"
                     /translation="CAAFGDSDLPYVLLATWPVPGCHRQVCAAFGDFDLPYVLLATWP
                     LPGCHRQVCAAFGDSDLPYMLTTPS"
     gene            complement(224..1546)
                     /locus_tag="P5316_21630"
     CDS             complement(224..1546)
                     /locus_tag="P5316_21630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87615.1"
                     /translation="MPNQLDRREFLKLTTAATAGTALLGAATNRAAEEQPQRTVRIGA
                     IGTGGRGCGLTRVMLSMKGVQLPAICDINPAALQAAKGLIVNAGQPEPAAYGDGPDAY
                     KKLLARDDLDAVLIATPWDLHAPIAVAAMKAGKYAASEVPCAIDMQQCWNLVNAHEET
                     GVPCMMLENWSFRRDNLAILNMIRKGLLGRIVHCHCAHSHDCLDHWFYDKQGNKRWGG
                     EYLIRRNCDQYPTHSLGPVLSWMDINCGDTYDYLTATASDPVGIYEYFKRTFGPDHPA
                     LKTQFKQGDIVTSVIRTKKGKTIVINYDMQLPRPYDNRWLIQGTLGLYREDPPTVYIL
                     GKSPQYHQWEPFAPYQEQYDHQWWRTHGKVAEAAGHGGTDYIELKTFLDAVRNKTQTP
                     IDVYDSVMMSVITPLSEQSIAKGSVPIKCPDFTKGKWETRKPTFAVEV"
     gene            1770..>2381
                     /locus_tag="P5316_21635"
     CDS             1770..>2381
                     /locus_tag="P5316_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87616.1"
                     /translation="MMNMLRCCLLVAVIAGCGTREAPPSLADEHEGPGNKLYMTPNDV
                     HALLLDETAEAFEQGTHSGTIVVSDPSGVTLETEPSNGEPARPPFKGVHTSRVIAAAF
                     PFNDVVPSWNVDCPLGAGFVVELRFGRAEGDFWTPFYYLGTWGTAEMPAVKIQHDRNG
                     DVDTDYFRSIHRFDRLQYRIHLYSPRRGPLPVLRRIGLAYSNTL"
BASE COUNT          383 a          693 c          781 g          524 t
ORIGIN      
        1 tctgcgccgc cttcggcgac tccgacctgc cctacgttct actcgcgaca tggcctgtgc
       61 caggctgtca tcggcaggtc tgcgccgcct tcggcgactt cgacctgccc tacgttctac
      121 tcgcgacatg gccgttgcca ggctgtcatc ggcaggtctg cgccgccttc ggcgactccg
      181 acctgcccta catgctgaca actccttcct gaaccctgaa cgctcacacc tcgacggcaa
      241 acgtcggctt tctcgtctcc cacttgccct tggtgaaatc cgggcacttg atcgggacac
      301 tgcccttggc gatggactgt tccgagagcg gcgtaatcac gctcatcatc accgagtcgt
      361 aaacatcgat tggcgtttgc gtcttgttgc ggacggcatc aaggaaggtt ttgagctcga
      421 tgtagtcggt tccgccgtgg ccggctgcct cggccacctt gccgtgtgtg cgccaccact
      481 ggtggtcgta ctgctcctgg tatggggcga aaggctccca ctggtgatat tgcgggctct
      541 tgcccaggat gtagaccgtc ggtgggtcct cgcggtacag gccgagcgtg ccctgtatca
      601 gccaacggtt gtcgtagggt cggggcagtt gcatgtcata attgatgacg atcgtcttgc
      661 ccttcttcgt acgaatcacg ctggtgacga tatcgccctg tttgaattgg gtcttgaggg
      721 ccggatgatc cggtccgaaa gtgcgtttga agtattcgta gatgcccacc gggtcgctgg
      781 cggtggcggt caggtagtcg taggtgtcgc cgcagttgat gtccatccag ctcagcaccg
      841 ggccgagcga atgggtcggg tactggtcgc aattccgccg gatgagatac tcgccgcccc
      901 agcgtttgtt gccctgttta tcgtagaacc agtggtcgag gcagtcgtgc gagtgagcgc
      961 agtggcagtg gacgattcgg cccaagagcc ccttgcggat catgttcagg atcgcgaggt
     1021 tgtctcggcg gaagctccag ttttcgagca tcatgcaggg tacacccgtt tcctcatggg
     1081 cgttgaccag gttccaacac tgctgcatgt cgatcgcgca gggcacctcg ctggcggcgt
     1141 atttgccggc cttcatcgcc gcgacggcaa tgggtgcgtg caggtcccag ggcgtggcga
     1201 tgagcacggc atcgaggtcg tcgcgagcca gcagcttctt gtaggcatcg gggccgtcgc
     1261 cataggcggc cggctcgggc tggccggcgt tcacaatcaa gcccttggca gcctggagtg
     1321 cggcggggtt gatatcgcag atggccggca gttgcacgcc tttcatgctg agcattaccc
     1381 tggtcaggcc gcatccccgc ccaccggtgc cgatggcgcc gatccgaacg gtcctttggg
     1441 gctgctcctc ggccgcccgg tttgtcgccg cccctagcag ggctgttccg gcggtcgccg
     1501 ccgtggtcaa cttcaagaat tctctgcgat cgagttgatt cggcatggtg acgagtcctt
     1561 ccagtgattg ctctttttca agtcggcgtg tcccggcggc atctgccggg gcggatgcgg
     1621 gtattatctg cggcttgcgc gcggcggcaa tgcgtccgat gatgtcgtgc aggcaggccg
     1681 ttgcggctat aatccggcga cgaggccggc gcggacggtc tgagaatgcc acatggatgt
     1741 tcaaggcgat acagagagga tgattgcggg tgatgaacat gttgaggtgc tgcctgttgg
     1801 tcgccgtcat tgcggggtgc ggcacgagag aggctccgcc gagtctggcg gatgaacatg
     1861 agggtccagg caacaagctg tacatgaccc cgaacgacgt tcatgctctg cttctggacg
     1921 agacggcgga ggcatttgag cagggaacgc actcgggcac gattgtcgtg agcgacccct
     1981 cgggcgtcac cctggagacg gagccttcga acggggagcc cgcgcgcccg ccgttcaagg
     2041 gcgtgcacac gtcgcgggtg attgctgcgg cgttcccgtt caacgatgtt gtgccttcgt
     2101 ggaacgtgga ctgtccgctg ggtgcgggtt tcgtcgtgga gctccggttt ggaagagccg
     2161 agggcgactt ctggacgccg ttctactatc tcggcacgtg gggaacggcg gaaatgccgg
     2221 cagtcaagat tcagcacgac cggaacggcg acgtggacac ggattacttc cgatcgatac
     2281 atcgttttga ccggttgcag tatcgcattc acctctattc gcccagaaga ggaccgttgc
     2341 cggtgctgag gcggatcggt ctggcctata gcaatacgct g
//
LOCUS       DAOTPW010000415         2361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_35194_length_2361_cov_5.365134, whole genome shotgun sequence.
ACCESSION   DAOTPW010000415 DAOTPW010000000
VERSION     DAOTPW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35194_length_2361_cov_5.365134"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="P5316_21640"
     CDS             complement(<1..604)
                     /locus_tag="P5316_21640"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HRR87617.1"
                     /translation="MAATNVPQFGVFEQSFTASGSYANPYTEASATGFFSRPGGGTLT
                     VPLFGEGGATWKLRFSPDALGTWTWFASSADSGLNGQYGQFSVVGSTLKGGIRRRAAT
                     PHHLEYQDAAVFWLFRDTNFGMMADNSAENSERSTIQSYISLRASQGFNLTHAELMNH
                     GGWSANTGGNPLASYMNETINPAHWQEVDGRVGDLNAAGIT"
     gene            complement(920..1990)
                     /locus_tag="P5316_21645"
     CDS             complement(920..1990)
                     /locus_tag="P5316_21645"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRR87618.1"
                     /translation="MAKRDSGTSRPFMRLEDIARAAGVSTATVSYALAGTKGVSAATR
                     RRILKIADELGYVPNRQASGLRAQRSLILGSLIPDIRNPFFADITAGLESAAASLNYR
                     LMLCVTEDDPADEARHLQMLLEHHVDGLVIVPVARSPEGAYPNLELLKLFQRRQIPII
                     SIVDGINELETGRITTAVYQGTRILADHLISLGHRDIAYFSQPFQRIQKYGRHTAYHD
                     ALREAGIPYHPELMVETGLSPGEAYRQTGRLLDSGVRFTAAMYPNDYMAVGGLRMFRE
                     RGIRVPDDVSVTGFDDIELARYCEIPLTTAAFPVRQLGEMAIRELVSLLGRADRGDEQ
                     PTVDVALRPTLVVRQSTGPPPA"
BASE COUNT          475 a          727 c          699 g          460 t
ORIGIN      
        1 cggtaatccc ggcggcattc agatcgccga cccgcccgtc cacttcctgc cagtgggcgg
       61 gattgatggt ctcgttcatg taactggcga gcggattgcc tccggtgttc gctgaccacc
      121 cgccgtggtt catcaattcg gcatgggtga gattgaagcc ctgcgatgcc cggaggctga
      181 tgtaggattg gatcgtcgat cgctccgagt tttcagccga gttgtcggcc atcatgccga
      241 agttggtgtc ccggaagagc caaaaaacag cggcgtcctg atactcgagg tggtgcggcg
      301 tcgccgcccg ccgccggatc ccgcccttca gggtgctccc aaccaccgag aactgcccgt
      361 actgtccgtt gaggccactg tcggcggagg acgcaaacca ggtccatgtt ccgagagcat
      421 cgggcgagaa cctgagcttc caggtcgccc cgccctcccc aaacagggga acggttagag
      481 tcccgccacc cggccggctg aagaagcccg tggcggaagc ctcggtgtaa gggtttgcgt
      541 agctgccgga tgctgtaaag gattgctcga agacgccgaa ttgtgggacg ttggttgccg
      601 ccaccgcgga cgcagctgcg caaagcggca gtaacaacct gaagccgacg agaacgacgc
      661 acgtatgctt ggagccgttc aatcttgtcg ccttgtccgc cgatgtgtca caagccattg
      721 tctgcgtgga ccacggaaat tcataccgcc aggcacgagc ctcgaccctc aggagaaagc
      781 aatctgatat gcatgaggta ttgaccttct tttcggcatt ggctgccctc acgcggaccg
      841 attttctcct cgagtgagag gatcccaaag ggcccctggc aatcagagag cccgacccag
      901 ccgtgcactc cacaggtact catgccggcg gcgggccggt cgactgccgc accaccagcg
      961 tcggccgaag ggccacatcg acggtcggct gctcgtctcc gcgatccgcc cgacccagaa
     1021 gtgacacgag ttcccgaatc gccatctctc ctaactgccg gaccgggaag gcggcggttg
     1081 tgagaggaat ctcgcaataa cgagctagct cgatgtcatc gaaaccggtg acggagacgt
     1141 cgtcaggcac ccgaatccct cgttcgcgaa acattcgcag accgccgacg gccatgtaat
     1201 cgttggggta catggcggcg gtaaaccgca cgccgctatc gagcagccgg cctgtctgcc
     1261 gataagcttc ccccgggctc agcccagtct cgaccatcag ttcgggatgg tagggaatac
     1321 cagcttcacg aagagcatcg tggtaggccg tatgccgccc atatttctgg atgcgctgga
     1381 acggctgcga gaaatacgcg atgtcgcgat gccccaaact gatcaggtga tccgccagta
     1441 tccgcgtacc ctggtagacc gcggttgtga ttcggccggt ctccaattcg ttaatgccgt
     1501 caacgatgga aatgatcgga atttggcgtc gctggaacag cttgagcagt tctaggtttg
     1561 gataagcacc ttccgggctc cgcgccaccg ggacgatcac cagcccgtca acatggtgct
     1621 ccaagagcat ctggaggtgg cgggcctcgt cggccgggtc atcctccgtc acgcacagca
     1681 tgagacgata gttgagggag gcggccgcac tttccaggcc ggcggtgatg tcagcgaaaa
     1741 acgggttcct gatgtcgggg atcaaagaac caaggatcaa gcttctttgc gcccgcaggc
     1801 cggaggcctg gcggttgggc acgtaaccca gttcgtcagc gatcttcaag attctgcgcc
     1861 gggtagccgc cgatacccct ttcgtgcccg ccagagcgta ggaaactgtg gcggtgctga
     1921 cacctgccgc ccgggcgatg tcctcaagac gcataaatgg cctgctggtg ccgctatctc
     1981 gtttggccat ctgcttaatc ctttcagttg cctatttcat tgtagcttca gaccgcggaa
     2041 attcaagacg ccgagtcact agttgagcag ctgattcgcg gcaaagaagg cgagcgcgcc
     2101 tgtccgagag cgagcgttct tggggagcag gaccaaaaca ctgcgacctg attgccaaac
     2161 acgcatgcaa gaagcttatc agtgaggatt aaagtcatga cccaaggtgg ccttcggcct
     2221 caacccaaga gccgtgctcg gcccgcaatc ctttccgggc cacctcctca ctcgcttggc
     2281 ccggaatcct ttccgggccg catcccctgc ggaatccttt ccgcattcgg cattcatgac
     2341 gatgtcaatc ctccaccgag c
//
LOCUS       DAOTPW010000416         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_35588_length_2342_cov_4.551377, whole genome shotgun sequence.
ACCESSION   DAOTPW010000416 DAOTPW010000000
VERSION     DAOTPW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35588_length_2342_cov_4.551377"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..949
                     /locus_tag="P5316_21650"
     CDS             71..949
                     /locus_tag="P5316_21650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRR87619.1"
                     /translation="MDYLNQVLQGDCGEVLKKLPDDTVDLIFTSPPYADQRKNTYGGI
                     SPQHYVEWFMPRAEQFRRVLKPTGTFILNIKERVVDGERHTYVIELILEMRRQGWLWT
                     EEFMWHKKNSHPGKWPNRFRDNWERLIQFNKSKAFNMYQEAVMVPVGDWAKDRLAKLS
                     QTDLTRDESKVGSGFGKNVSNWVGREMVYPNNVLHMATECYNRNHSAAFPVDLPKWFI
                     KLFTQPGDLVLDPFLGSGTTAVAALQLGRNFVGIELMPENVATANARISETQIALPLL
                     IADEQAEYQAGTDVSS"
     gene            988..1728
                     /locus_tag="P5316_21655"
     CDS             988..1728
                     /locus_tag="P5316_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HRR87620.1"
                     /translation="MNQLNIDDVLQYVEQNIDIFHQKRIQSLDSLKLSQVLKRKNPYL
                     FRAKHVFTPEQFVTGLVDAHLSSHEETIFGDWLEQLAIFINGKVYGGWKSGITGIDLE
                     FDADGRRNIVAIKSGPSWGNSSQIAKMVSDFKSAQRTLRTSNSQLHVVAVNGCCYGKD
                     DRPDKGDYFKFCGQRFWAFISGSNELYTDIIEPLGHRARERNDEFIESYWAMITRFAR
                     EFETDFCSDCRIDWVRLVRYNSQARLSV"
     gene            1828..>2342
                     /locus_tag="P5316_21660"
     CDS             1828..>2342
                     /locus_tag="P5316_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87621.1"
                     /translation="MLTSRFRPPLWLAALPLLVLCLAPACGLDAAPAPPDPRFGIVET
                     FVNPDAASEAGAGYTRAILRWDVIQPDSPADWKPANVPDPLIAGELADGREVVGLLIG
                     TPVWAAAPGQTPGSRAVPDMAAWEAFARRMAQHYRGRIRHWVIWNEPDVWQEGHPGRT
                     WDGTVEDYALLL"
BASE COUNT          482 a          697 c          726 g          437 t
ORIGIN      
        1 caggttatgg gatagcttct atagcgtacg tacctcgaaa cgtgcgtgat ggtgttgtgg
       61 agagaagatg atggactatc tgaaccaggt tttacagggc gattgcggtg aggtgttgaa
      121 gaaactgccc gatgacacgg tagatctgat cttcacgtcg ccgccttacg cggatcagcg
      181 caagaatacc tacggtggca tcagtcctca acactacgtg gagtggttca tgccgcgcgc
      241 cgagcagttt cggcgcgtgc tgaagcccac tgggaccttc atcctcaaca tcaaagaacg
      301 ggtggtcgat ggagagcggc acacctacgt catcgagctg atcctggaga tgcgcagaca
      361 gggctggctg tggaccgagg agttcatgtg gcacaagaag aactcgcacc ccggcaagtg
      421 gcccaaccgg tttcgcgaca actgggaacg attgatccag ttcaacaagt ccaaagcgtt
      481 caacatgtac caggaagcgg ttatggtccc ggtgggtgat tgggccaagg ataggctggc
      541 caagctcagc cagaccgacc tgacgcggga tgaatcgaag gtgggcagcg gcttcggcaa
      601 gaacgtctcc aactgggtcg gccgcgagat ggtgtacccg aacaacgtcc tccacatggc
      661 caccgaatgc tacaaccgga atcacagcgc ggccttcccg gtcgatctgc ccaagtggtt
      721 catcaaactg ttcacgcaac ctggcgatct cgtcttggac ccgttcctcg gttccggcac
      781 gacggcggtg gccgcgttgc aattgggacg caacttcgtg ggcatcgaac tgatgcccga
      841 aaacgttgcg acggcaaacg ccaggatatc cgagacccaa atcgccctgc ctctgctgat
      901 cgccgacgaa caagcggagt atcaggccgg aaccgatgtt tcgtcatagg cgatgtcgcc
      961 acagagaact ttcgagggag agctgcgatg aatcagttga acatcgatga tgtcctgcaa
     1021 tatgtcgaac agaacatcga catcttccac cagaagcgga tacaaagcct cgacagcctg
     1081 aagttgtctc aggtgctcaa acgcaaaaat ccatatctgt tccgggcaaa gcacgtgttc
     1141 acgcctgagc agttcgtcac gggtctggtg gacgctcacc tgtcgtcgca cgaggagacg
     1201 atctttggcg attggctcga acagctcgcc atcttcatca acggcaaggt ttacggcgga
     1261 tggaagtccg gcatcaccgg gatcgatctc gagttcgacg ccgatggcag aaggaacatc
     1321 gtggccatca agtcggggcc gagctggggt aacagttcgc agatcgcgaa gatggtctcg
     1381 gatttcaaat ctgcccaacg taccctgcga accagcaatt cgcaacttca tgtggtggct
     1441 gtgaacggct gctgttacgg caaggatgac cggcccgaca agggcgacta tttcaagttc
     1501 tgcggccagc ggttttgggc gttcatctcg ggcagcaacg agctgtatac cgatatcatc
     1561 gaaccgttgg ggcatcgggc cagggagagg aacgacgagt tcatcgagtc ttactgggcc
     1621 atgatcaccc gtttcgcccg cgaattcgaa accgacttct gcagcgactg ccggatcgat
     1681 tgggtgagac tggtgcgcta caactcgcag gcccggctgt ccgtttgagg gcgttgtcgg
     1741 acacatttct ctcaacccgc tgcccggccg gcttaacggg tgaagttagg ccgctggagg
     1801 aacagtcgca ccgccacctt cgactctatg ctcacctctc gatttcggcc acctctttgg
     1861 ttagcggcgc tgccgctgct cgtgctgtgc ctggcgccgg cgtgcggcct cgacgccgcg
     1921 cccgccccgc ccgacccccg tttcggcatc gtggagacct tcgtcaaccc ggacgcggcc
     1981 agcgaggccg gcgcgggcta cacgcgcgcc atcctacgct gggacgtgat ccagcccgac
     2041 agcccggcgg actggaagcc ggccaacgtg cccgacccgc tgatcgccgg cgagctggcg
     2101 gacggccgcg aggtggtcgg cctgctgatc ggcacaccgg tctgggccgc ggcgcccggc
     2161 cagacgccgg gcagccgcgc cgtccccgat atggccgcct gggaggcctt cgcccgccgc
     2221 atggcccagc actaccgggg ccgcatccgc cactgggtga tctggaacga gccggacgtc
     2281 tggcaggagg ggcatccggg caggacctgg gacggcacgg tggaggacta tgcactgctg
     2341 ct
//
LOCUS       DAOTPW010000417         2272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_37048_length_2272_cov_4.343708, whole genome shotgun sequence.
ACCESSION   DAOTPW010000417 DAOTPW010000000
VERSION     DAOTPW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37048_length_2272_cov_4.343708"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="P5316_21665"
     CDS             <1..168
                     /locus_tag="P5316_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRR87622.1"
                     /translation="VHKAMRPPKFKCRIVRRCQICGRKHAVYRKFRMCRVCFRKFANE
                     GLIPGVKKASW"
     gene            236..631
                     /gene="rpsH"
                     /locus_tag="P5316_21670"
     CDS             236..631
                     /gene="rpsH"
                     /locus_tag="P5316_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRR87623.1"
                     /translation="MWSDPIADMLTRIRNVAMARGREVKVPASRVKAGIAEVLKQEGY
                     IQEYDRIDDGRQGILRIKLKYGRRGEPVLTEIKRISKPGRRDYRAVEDLPEVLNGLGI
                     AIVSTSQGVLSDRQCRERHIGGEVLCTVC"
     gene            686..1300
                     /gene="rplF"
                     /locus_tag="P5316_21675"
     CDS             686..1300
                     /gene="rplF"
                     /locus_tag="P5316_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRR87624.1"
                     /translation="MSRIGKKPIPVPSGVQVIVKDHTVTAKGPKGELSWRFPDEITVQ
                     ADSGAGLIVVGRTDDSSRVKALHGLTRALIANMVAGVSKGYEKRLEIYGTGYGCQVKG
                     GKFLLNCGYMGRGVDAEGKPKEAQYVIDIPKGLTVTVEVPTARGESEPARFTVTGADK
                     QLIGQFTSELRLIRPPEPYKGKGLRFVGEHVRRKQGKAFAGGAS"
     gene            1330..1698
                     /gene="rplR"
                     /locus_tag="P5316_21680"
     CDS             1330..1698
                     /gene="rplR"
                     /locus_tag="P5316_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRR87625.1"
                     /translation="MSMIKVKQQRRQRRKMRVRRRVFGTAERPRLTIFRSLKNIYAQM
                     IDDETGRTLVEASSVSKELAGVVKYGGNVAAAAQVGDLLAQRALAKGVKQVTMDRNGY
                     RFHGRVKAVAEAVRKAGIKI"
     gene            1761..>2272
                     /locus_tag="P5316_21685"
     CDS             1761..>2272
                     /locus_tag="P5316_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRR87626.1"
                     /translation="MADNQTNETQGEVSGEQVTHSAASRLAGRAPEPPEGGSFAPEGE
                     RRERDERGERGRGPGRPERTGPADGLDEVVVKIYRCATVVKGGRRFSFGALVVVGDRN
                     GRVGIGYGKANEVPNAVEKGAKAARRNMISVNLDGSTIPHMVFSKYRASRVKMIPAGE
                     GTGVIAGASVR"
BASE COUNT          462 a          594 c          840 g          376 t
ORIGIN      
        1 gttcacaagg caatgcggcc tccgaagttc aagtgccgga tcgtgcgccg gtgccagatt
       61 tgcggccgca agcacgcggt gtaccggaaa ttcaggatgt gccgcgtttg tttccggaag
      121 ttcgcgaacg aaggcctgat tccgggcgtg aagaaggcga gttggtgagc gtcgcgaggc
      181 cccggtggtt gccggggtac tagcaggacg gccggcaaga aagaggactg agacgatgtg
      241 gagtgacccg atcgccgaca tgctgacgcg gattcgcaac gtggccatgg cccgcggccg
      301 cgaggttaag gttcccgcgt cgcgcgtgaa ggccggcatt gccgaggtac tcaagcagga
      361 aggatacatc caggaatacg accggatcga cgacgggcgt caggggatcc tgcggatcaa
      421 gctcaagtac ggtcgccgcg gcgagcccgt gctgacggag atcaagcgga tttccaagcc
      481 tggtcggcgc gactatcggg cggttgagga cctgccggag gtgctcaacg gtctgggcat
      541 cgcgatcgtc tcgaccagcc aaggtgttct gagcgatcga cagtgccggg aacggcacat
      601 cggcggtgag gtactctgta cggtgtgctg acggggcgag gaatgtatcc ggcggaagcc
      661 ggacggtttg gaaggactaa agacgatgtc tcgtatcggc aagaaaccca ttccggtgcc
      721 ctccggtgtg caggtcatcg tgaaagacca caccgtgacg gccaaagggc ccaaggggga
      781 gctgtcgtgg cggttccctg acgagatcac ggttcaggcc gactcgggag ccggcctcat
      841 cgttgtgggg cgcacggacg attccagccg ggtcaaggcg cttcacgggc taacgcgggc
      901 gctgattgcc aacatggttg ccggcgtgtc caagggctat gagaagaggc tggagattta
      961 tgggaccggc tacggctgcc aggtgaaggg aggcaagttt ctgctcaact gcggatacat
     1021 gggtcggggc gtcgatgcgg agggcaagcc gaaagaggcg cagtatgtca ttgacattcc
     1081 caaggggttg acggtcacgg tggaagtgcc cacggcgcgc ggtgaaagcg agccggcgcg
     1141 gttcaccgta accggcgcgg acaagcagtt gatcgggcag ttcacgtcgg agctgcggtt
     1201 gattcggcct cccgaacctt acaaaggcaa ggggcttcga tttgtgggcg agcacgtgcg
     1261 gaggaaacag ggcaaggctt ttgccggcgg ggcgagttga cccgcagacg gagagtatcg
     1321 ggtggcagca tgagcatgat caaggtcaag cagcagcgtc gtcagcgccg caaaatgcgg
     1381 gtgcggcggc gcgttttcgg taccgcggag cggccgaggc tgacgatctt ccggagccta
     1441 aagaatatct acgcgcaaat gatcgacgac gagaccggac gcacgctggt cgaggccagc
     1501 agcgtcagca aggaactggc gggcgtggtg aaatacggcg ggaacgtcgc cgccgccgca
     1561 caggttggcg acctgctggc acagcgggcc ctggccaagg gtgtgaagca ggtgaccatg
     1621 gaccgcaacg gttacaggtt ccacgggcgt gtcaaagcgg tggccgaggc ggttcgcaag
     1681 gcgggtatca agatctgagg tgttaggatg aaccggtttt tcggcgagag ggcggcgagc
     1741 cggttgtgat cgaggcgctg atggcagata accagacgaa tgagactcag ggcgaggttt
     1801 ccggcgagca ggtaacccat tcggcagcgt cacggctggc cggccgggcc ccggaacctc
     1861 ccgaaggcgg ctcgtttgcc cccgagggcg aacgtcggga gcgagacgag cgaggcgaac
     1921 ggggccgtgg tcccggtcgt cccgaacgaa ccggacctgc cgacgggctc gacgaggtcg
     1981 tggtcaagat ttatcggtgt gccaccgtgg tgaagggcgg tcggcgattc agctttgggg
     2041 ccctggtggt tgtcggcgac cgcaacggtc gagtgggtat cggctatgga aaagcgaatg
     2101 aggttcccaa tgccgtggag aagggtgcca aggccgcccg gcgaaacatg atcagcgtga
     2161 atctggacgg gtcgacgatt cctcacatgg ttttttcaaa atatcgagcc agcagggtga
     2221 aaatgatccc cgccggcgaa ggaaccggcg ttatcgcggg agccagcgtg cg
//
LOCUS       DAOTPW010000418         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_37067_length_2271_cov_5.510830, whole genome shotgun sequence.
ACCESSION   DAOTPW010000418 DAOTPW010000000
VERSION     DAOTPW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37067_length_2271_cov_5.510830"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="P5316_21690"
     CDS             complement(<1..376)
                     /locus_tag="P5316_21690"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRR87627.1"
                     /translation="MSSNGTKRVHLAYGKTGLDVQVPADAVVIEPKYVKGLPDERGAL
                     IEAMRRPIGTPPLRDLVKAGQTVTIVHTDITRATPNERILPPLLAELESAGIKRSDII
                     LLNALGTHRPQTREELEVMLGKE"
     gene            complement(1476..2024)
                     /locus_tag="P5316_21695"
     CDS             complement(1476..2024)
                     /locus_tag="P5316_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005371778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR87628.1"
                     /translation="MPNYIRWRREGASYFFTVVTYRRRRVFDTPLGRRLLRQAITETR
                     NRWPFDVFAMVLLPDHLHCLWTLPRDDDRFPARWGHIKKTFTQRYVAAGGKPLSVSKN
                     RARHREQGIWQQRYWEHRIRDEEDWYRHRDYIHLNPVKHGYVSEPQTWPWSSIHRHIQ
                     LGWLDPNWPGSSPIEIPDVLGE"
BASE COUNT          432 a          697 c          668 g          474 t
ORIGIN      
        1 cctccttgcc gagcatgacc tccagctcct cgcgggtttg aggccgatgg gtgccgagcg
       61 cgttgagcag gatgatgtcc gatcgcttga tgcccgccga ttcaagctcg gccagcagcg
      121 gcggcaggat ccgctcgttc ggcgtcgccc gggtgatgtc ggtgtgaacg atggtcaccg
      181 tttggccggc cttcaccagg tcgcgcagcg gcggtgtacc gataggcctg cgcatcgctt
      241 cgatcaaggc gccccgctcg tcgggcaaac ccttgacgta cttgggctca attacaacgg
      301 cgtctgccgg cacctgcacg tccaagccgg tcttgccgta ggccagatgg actctcttcg
      361 ttccgttgct gctcatgctt acctcgcgat caaggcgggc aaagcccgcc ctccatggtg
      421 ccggacggcg gttctccggt aggcgggctc cgcccgccgt catgtctgac cgcattgtaa
      481 ccgcggttgg gataaacagg gtagggcaga cttcgcccgt cgacccgctc tggtagggcg
      541 ggctttgcct gccgatttcc acgatgtctc gcggtcagga tgcccagacc agagcaggcg
      601 atcaccgggc cagatctcgt agcaccaatc gtgcgggtag tcccagaaga ggattctgtc
      661 gaaccctgag catggggccg cctttctcag gtcgggatca ttccattctg cgatctgttc
      721 gggcgcggaa cactgggccg gcgttgcacc agaggccgtg atcaataacc acttcttctc
      781 ggcgcttctc cagttgtacg ttgccgattt gcgggtcttg cactggatcg cagccgtcaa
      841 gtgcctcaca atgaaccctg tgtcaccagc tgtcagattg ctgcatgtcc aggccatacg
      901 ccagctctgc catatgtccg attctgccct catgaaccgc acctggctca gtaggcttga
      961 caagcgggga cactccggag ggaaagtcct caaggtccgg tgttctccgg gcgtcagagg
     1021 attcgacagg gccagacgga ccagttcggc cgcaacatcg cgagcaagct gcaatcgtgc
     1081 tgtgggcgtt cgtggcggct tcggaaacgg atccctgaac tctacaatca ccattaaact
     1141 gcggaggtgc tgttgatagt tgagcctgcg acagaggctg gtctgcacta tcctccagaa
     1201 ccacccaagg cccgtcaaag gggatacctt acccggcagc acatcgttga agtagtccat
     1261 gtgctcgatg ccaagaatcg ttttgcttcg atccttgacg acggtcagta aagcatccgg
     1321 tcgttcctgc catgtggcgc gagtgattcg gtaacccatc ctgcgcaaga gccgtcgaat
     1381 cagaaactgt tcatactcct tgggagttcg gtacactctt ggcattggcc aatactccgg
     1441 tcaaggcgac ggcgggcaca gcccgcccta ccgagtcatt cgcccagcac gtcgggaatc
     1501 tcaattgggc tcgaaccggg ccagttggga tccagccacc cgagttgaat gtgccggtgg
     1561 atgctcgacc aaggccaggt ttgtggctcg gagacgtacc catgcttgac cgggttcagg
     1621 tggatgtagt cgcggtgtcg gtaccagtct tcctcgtcgc gaattcgatg ctcccagtac
     1681 ctctgctgcc agattccctg ttccctgtgt ctcgcacgat tcttggacac cgacagcggc
     1741 ttgccaccgg ctgcaacgta ccgttgcgtg aacgtcttct tgatatggcc ccagcgagca
     1801 gggaatctgt catcatccct gggcagtgtc cacaggcaat gaaggtgatc cggcagaaga
     1861 accatcgcaa acacatcgaa aggccagcgg tttcgagtct ccgtgatcgc ctgcctcaac
     1921 aaccgccggc ccaaaggtgt atcgaacact cgtcgacgac gataagtcac cacggtgaag
     1981 aagtagcttg ctccttctct gcgccagcgt atatagttgg gcatgggaac agcctagcag
     2041 aataccagaa ataaagaaag gcagaaggcg ggcacagccc gccctaccgt ctcgccgcgc
     2101 ggagccgttg tcatggcacc cagcggaccc atgcgaatca ggtgttcttc aggtacttgt
     2161 cgatcgccgc ggccgagttc ttgccgtcac ccatggccag gatcaccgtg gccgccccgc
     2221 gaacgatgtc gccgccggcg aaaacgcccg gaatgctggt cttgttgttg g
//
LOCUS       DAOTPW010000419         2258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_37336_length_2258_cov_3.875170, whole genome shotgun sequence.
ACCESSION   DAOTPW010000419 DAOTPW010000000
VERSION     DAOTPW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37336_length_2258_cov_3.875170"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(546..>2258)
                     /locus_tag="P5316_21700"
     CDS             complement(546..>2258)
                     /locus_tag="P5316_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87629.1"
                     /translation="LTPAVSVPGPLWAGETTVTVTNVLDTATEVRVYKAGYTQIGRKV
                     LTSPSTGTEVVTLAEPLVQGSIISATQTVNGIEGCQTVVGATVGPECGRTPAVSVQSP
                     LISTQTVVYVAGVSVDPAATEVRVYDGTTQIGANTAPGGLATVPVTVTQLVSGHTIKA
                     TQVVNGVEGCLNTAPSLVVDNCNQVPAVNVVGPVNAGATQVTVGGVATSTAEVTVSAV
                     TVYANGAQIGQKTSGIVAGNNVVDVTPLVKGQNITATQTFTTAYGSLEGCKPTTDEMV
                     GSGGNSQVLVSLGVRFTGPTGGIGENGGISGTITWLVQSATEGAPPIGKALAPLPMWQ
                     TLTFDPATDAKTPFTGDWPSPAPTKAVLEELAFTIDQAAPNVGPYMIYIDNITSGGVN
                     FADFESIPAGQQAVFRQPSYSGSTSGNIAAAPNLSVVDGTVGYGGGQSCRIEWAFKDE
                     ALTRWLRLTTNDTPNIPNPIIDLTQPITMQVLFQPLGAKPCNDPFADVDDDGDVDQAD
                     FAVMQRCIGLPLPPGEGEDPTPLPAECFCFDRAHDGDVDSDDVGFFEACASGAGIAAD
                     PDCD"
BASE COUNT          440 a          678 c          720 g          420 t
ORIGIN      
        1 aatgcggaaa ggattccgcg ggggatgcgg cccggaaagg attccgggcc gagctggttg
       61 ggttcgtttg gtcagaaaga gtcttgaact gttggctgtt ggtttgggcc ggaggaggtt
      121 ggtcttcaga tttcaaagag cgggcaaggg agctttcagt gatcagctct cagttgtcag
      181 ccagacaagg gagagaacaa gaggggtgct ccggaaaggg cggttggagc ggagcggcga
      241 cgtggctcac agccttgggc tgtcggctcg ttgctcgtgg ccggggccgg aggaggtgtg
      301 gggcgaaaga gggcattggc ggctccagaa tcccctctgg ggtcggcgaa aatggattcc
      361 cggggccggc gagcgctgag cacgcatgat gccggtctca tacgacggat gatgatcgtc
      421 cgtgcgacct ggaaaggcgc gagttgcaca ccggttccgg cacgggccgc aaaaagaacc
      481 ggggaccggc cccatacaga accggtcccc ggaagggatt acagactact caaccgatgc
      541 cgcgatcagt cgcagtccgg atcggcggcg atacccgcgc cgctggcgca ggcctcgaag
      601 aagcccacgt catccgagtc gacatccccg tcgtgggcac gatcgaagca gaagcattcg
      661 gccggcagcg gcgtcggatc ttcaccctcg ccaggaggca agggcaatcc aatgcacctc
      721 tgcatgaccg caaagtcagc ctgatccaca tctccgtcat catcgacgtc ggcgaacgga
      781 tcattgcacg gtttcgcgcc aagcggctgg aagagaacct gcatcgtgat cggctgcgtc
      841 aggtcgatga tcgggttggg gatgttcggc gtgtcgttgg tggttagccg gagccaccgc
      901 gtcaaggcct catccttgaa cgcccactca attcgacacg actgaccacc gccgtagcca
      961 acggtcccgt ccaccacaga caggttcggc gccgccgcga tgtttccgct cgtgctgcct
     1021 gagtaactcg gctgacggaa gacggcttgc tgtcctgccg ggatcgactc gaagtcggca
     1081 aagttgacgc cgccgctggt gatgttgtcg atgtagatca tgtaaggtcc gacatttggc
     1141 gccgcttggt cgatggtgaa cgccaattcc tccagcacag ccttggtggg agcaggactc
     1201 ggccaatcac ccgtaaacgg cgtcttcgca tcggtggcgg gatcaaaggt gagcgtctgc
     1261 cacataggca gcggtgcaag ggccttgccg atgggaggcg ccccctccgt ggcactctgc
     1321 acgagccacg taatggtgcc gctgatccca ccgttctcgc caattccgcc agtcggacct
     1381 gtgaaacgga cacccaagga taccaggact tgggagttac cgccggagcc gaccatctcg
     1441 tcggtcgtgg gcttgcatcc ttcaagcgag ccataggccg tggtaaaggt ctgggttgcg
     1501 gttatgttct ggcccttcac caaaggagta acatccacga cgttgttccc ggccacgatg
     1561 ccactggtct tctgcccgat ttgggcaccg tttgcatata ccgtcaccgc agatacggtc
     1621 acctcggcag tcgaggtggc cacaccgccc acagtcacct gcgtcgcacc ggcgttgacc
     1681 ggcccgacca cattgactgc cggcacttgg ttgcagttgt caaccacaag actgggagcg
     1741 gtgttaaggc aaccctcgac gccgttaacc acttgggtgg ccttaatggt atggccgcta
     1801 acgagctgcg tcacggtaac gggcacggtc gcaagaccac cgggagccgt gttggcgccg
     1861 atctgggttg ttccgtcgta gaccctgacc tcggtggcgg ccggatccac gctcacacct
     1921 gcgacgtaaa ccacggtctg cgttgagatg agaggactct ggaccgaaac ggccggcgtt
     1981 cgaccgcact cgggcccgac cgtggcaccc accaccgtct gacagccttc gataccgttg
     2041 acggtctgcg tggcggaaat aatgctgccc tgcacaagcg gctcggcaag cgtcacaacc
     2101 tcagtgccag tggaaggact ggtcaagacc tttcgaccga tttgcgtgta accggccttg
     2161 tagacccgca cctctgtcgc cgtgtcgagc acgttcgtca cggtaacagt ggtttcaccc
     2221 gcccacaacg gaccgggcac actgacggcc ggcgtcaa
//
LOCUS       DAOTPW010000420         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_37400_length_2255_cov_6.160455, whole genome shotgun sequence.
ACCESSION   DAOTPW010000420 DAOTPW010000000
VERSION     DAOTPW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37400_length_2255_cov_6.160455"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="P5316_21705"
     CDS             complement(<1..1676)
                     /locus_tag="P5316_21705"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRR87630.1"
                     /translation="MAKRFFLIDGLSQIFRCYYAPFQNLSSPAGEPTKATYVFSNMLL
                     QLLREQKPDYLAMVMEGGDEKDVFRKELDPQYKANREPPPEDLAPQIERILQIVRSQG
                     IPVLSVPGFEADDLLATLAHRLSGEDLEVIIVSRDKDLDQIVNERIRLYDPMKNEFLG
                     PREIETQKGYRPEQAVEVQTLVGDSTDNIRGVRGIGPKKAAALIAKYGTAKAVIEHAD
                     ELTPAMKKNVLEFADRLDTTRQLVTLRRDVPLNLDLESCRFQGIKPAVLKQVFAELGF
                     RRLTDLMAAEAGPPQTPQSPAPRAATRPDSAAQAQLSLFGDEDSGTIPPPPQLKPSLP
                     QERPSGRDYRLIDDQNQFRDFLTRLREQPVFAVDTETTSLSPVDAQLAGLSFSWQANT
                     GYYLPLRGVGRCLPVEPTLEALRPILADPNVRKIGQNIKYDLVVLKHHGVEVAGVAFD
                     TMIASFVLDSTRRSHGMDALARELLGFDPIPISDLIGKGSKQIRFDQVDTATACEYSA
                     EDADVTWQLYEVLSKQLAGSPSEPLFRDTEMPLVTVLADMEAEGIRLDTS"
BASE COUNT          420 a          660 c          725 g          450 t
ORIGIN      
        1 atggacgtat caagccggat tccttcggcc tccatatcgg ccagtaccgt caccagcggc
       61 atctcggtgt cgcggaacaa cggctcagag gggctgcctg caagctgctt gctcaagacc
      121 tcataaagct gccaggtgac gtcggcatcc tcggccgagt actcgcaggc agtcgccgtg
      181 tccacctggt caaagcggat ctgtttgctg cctttgccga tcaggtcgct gatgggaatc
      241 gggtcgaagc cgagcagctc gcgggccagg gcgtccatgc cgtgcgaacg ccgcgtcgag
      301 tcgagcacga aactggcgat catggtgtcg aacgcaacgc cggcgacctc gacgccgtgg
      361 tgcttgagga ccacgaggtc gtatttgatg ttctggccga ttttcctcac gttcgggtcc
      421 gccaagatcg gccggagggc ctcgagcgtg ggctcgacgg gcagacatcg cccgacgcct
      481 cggagaggca ggtagtagcc ggtgttggcc tgccatgaaa aactgagacc cgcgagttgg
      541 gcatcaaccg ggctgaggct cgtcgtctcg gtgtcgacgg cgaagacggg ctgttctcgc
      601 aatcgagtaa ggaaatcgcg gaactggttc tggtcatcga tcaagcggta gtcgcggccg
      661 ctcggtcgtt cctggggcag gcttggcttg agctgcggag ggggtgggat cgttccggag
      721 tcttcgtcgc cgaacaggct cagttgtgct tgtgctgctg agtcgggccg ggtggcggca
      781 cggggggcag gggattgggg tgtctggggc ggacccgcct cggcggccat caggtcggtg
      841 agacggcgga agcccagctc ggcgaaaacc tgcttcaaaa cggcgggctt gatgccctgg
      901 aaccggcaac tctccagatc aagattcaga gggacatcgc gtctgagggt caccaattgc
      961 cgggtggtgt caaggcggtc ggcaaactcg aggacgttct tcttcattgc cggagtgagt
     1021 tcatcggcgt gctcgatgac cgccttcgcg gtgccgtatt tggcgatgag ggcagcggcc
     1081 ttcttgggac cgatcccgcg aactccccgg atattgtccg tgctgtcacc aaccagagtc
     1141 tgaacctcga cggcctgctc cggccggtag cccttctggg tctcgatctc ccgcggtcca
     1201 aggaactcat tcttcatcgg atcgtagagg cgaatgcgct cgttcacgat ctgatccaag
     1261 tccttgtcac gactcacaat aatgacttcc agatcctccc ctgaaagccg atgggcgagc
     1321 gtggccaaca gatcgtcggc ctcgaaccca gggacgctga gcaccggaat tccctgggat
     1381 cgcacgattt gcaggatgcg ttcaatctgc ggggccagat cctcgggcgg aggttcccgg
     1441 ttggccttgt actgggggtc gagttccttg cggaacacgt ccttctcgtc tccgccttcc
     1501 atgaccattg cgaggtagtc gggcttctgc tcccggagga gttggagcag catgttcgag
     1561 aacacgtacg tggccttggt tggctctccg gccgggctgc tcagattctg gaaaggggca
     1621 tagtagcagc gaaatatctg cgagagaccg tctatcagga agaaccgctt ggccatgtgc
     1681 tgctcatttc gtgcaaggaa cgatctagcc gcactgtagg acggctgcgt cgggggtgtc
     1741 aagccgactt ctgtgatttc cgcatgtttg atcgggctgc ggggccggac acctctttgc
     1801 cgccccttga ggtcggccgt tcagcgttat cgcgttttgc cggccttaag agccgggtgc
     1861 aggacgtgat gcggtcggct cggggcagcc ggtggggcaa tcgcatctca atcccctggc
     1921 ataggacccg ggtcaggcga tcctcccgca agccgctcca tccggggatc ctccaactca
     1981 tcaaaaaagc gcagcaggcc acgcgcagct ccaacgtcca tgttgcagct cctgaaaaag
     2041 gaaaaacctc tagaaagcgc aaggcctcat aaccactatg cccgaagtta tccgcaattc
     2101 agccagagcg caatccttac agatgcccaa ggcggaccgc ctttttgcgg gttgcaggcc
     2161 aagcgcgctt aaacccgcaa taggcgcgca aaccgcgcag atgtccggcc gtaaggattg
     2221 aaaggcgcat gcccacgcaa agccgtgggc atggc
//
LOCUS       DAOTPW010000421         2252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_37476_length_2252_cov_4.628129, whole genome shotgun sequence.
ACCESSION   DAOTPW010000421 DAOTPW010000000
VERSION     DAOTPW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37476_length_2252_cov_4.628129"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1044)
                     /locus_tag="P5316_21710"
     CDS             complement(292..1044)
                     /locus_tag="P5316_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRR87631.1"
                     /translation="MTDPQLQAIVSEQPYPLLFVTISGAHLYGFPSPDSDYDLRGVHI
                     LPAREVVGLSEPRETIELSGFRDGREIDLVTHDVKKFFLLLLKKNGYVLEQIYSPLVV
                     HTTPEHEELKEIAKGCVTRHHVHHYLGFAETQWNLFTKDSSPRVKPLLYIYRVLFTGI
                     HLMRTGQVEANLVRLNEGFKHPAIADLIARKMAGPEQATLPDTEMGFHEREYHRLRNE
                     LEQAGQKSGLPEAPSTRDALNDLLVRVRMGPQ"
     gene            complement(1041..1979)
                     /locus_tag="P5316_21715"
     CDS             complement(1041..1979)
                     /locus_tag="P5316_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRR87632.1"
                     /translation="MNGTRSNPPPILPVGTQIVTRVEIAASAGQPGQPRGAVGIVLRS
                     PADSTHAYRVRFVNGGEATLHRKDFSVRKQHQEEGLRRGPDDGNDQDLTQFVIYRCIS
                     GSRAYGLDNEESDTDRRGIYLPPAERHWSLYGVPEQLENTETEECYWELGKFLILALK
                     ANPNVLECLYTPMVELATPLARELLDMKAAFLSRLVYQTYNGYVMSQFRKLEQDLRNK
                     GAIKWKHAMHLIRLLLSGITVLREGFVPVRVDEYRDRLLAIRDGRTPWEEINAWRLDL
                     HQRFDDAFTDTRLPEQPDYHKIDAFLIKARRSMVPS"
BASE COUNT          400 a          702 c          694 g          456 t
ORIGIN      
        1 gaggattctc atcctcattc tcgctggccc cagaatccgt tctggggcct tccggatgga
       61 ggattctcat cctcattacc ttcaccgaac gcagaacaaa taccgccagg cacggccgcc
      121 cggcacgggc acgtagcgca ggccacagcc cagcggcgac ctgccaagca tgtagggcag
      181 gtcacagcgc agcggcgacc tgccttcccg ctcaagatgt tgcggcaggt ctcgcttcgc
      241 tcgaccatgc cctaccaccg gctccgggac gggccgacga gggcattggc cctactgtgg
      301 acccattcgt acccgtacca gcagatcatt caacgcatcc cgtgtcgatg gagcctcggg
      361 caggccgctc ttctgtccgg cctgctccaa ctcgttccgc agacgatgat actctcgctc
      421 atggaagccc atttccgtat ccggcagcgt cgcctgctcg gggccggcca tcttgcgcgc
      481 gatcaggtcg gctatggccg ggtgcttgaa tccctcgttg agccgtacca ggttggcctc
      541 cacctggccg gtacgcatca ggtggattcc ggtgaatagc acgcggtaga tgtagagcag
      601 cggcttgact cggggcgacg agtccttggt gaacaggttc cactgggtct ccgcgaaacc
      661 gaggtaatgg tgcacatggt ggcgggtgac gcagcccttg gcgatctcct tcaactcctc
      721 atgttcgggt gtcgtgtgga caaccagcgg cgagtagatc tgttcgagca catagccgtt
      781 cttcttgagc agcaacagga agaacttctt gacatcgtgc gtgaccaggt cgatctcacg
      841 gccgtcgcga aaaccgctga gctcgatggt ttcccgaggt tcactcaagc caaccacctc
      901 gcgggccggc aggatatgga cgccgcggag gtcgtaatca gagtcgggcg aaggaaatcc
      961 gtacagatgc gccccgctga tggtgacgaa gagcagaggg taaggctgct cgctgacaat
     1021 cgcctgcaac tgcggatcgg tcatgatggc accatgcttc tacgggcctt gatcagaaac
     1081 gcatcgatct tgtgatagtc cggctgctcc ggcagacgcg tgtccgtgaa agcatcgtcg
     1141 aaccgctggt gaagatccag ccgccaggca ttgatctctt cccacggcgt gcggccgtcg
     1201 cgtatcgcca gcagccggtc acgatactcg tcgacccgca ccggtacgaa gccttccctg
     1261 agcaccgtga tgcccgacag gagcaggcgg atcaggtgca tggcgtgttt ccacttgatc
     1321 gcccccttgt ttcgcaggtc ctgctccagc ttgcgaaatt gcgacatgac atagccgttg
     1381 taggtctggt agaccaggcg tgagaggaac gcggctttca tatccagcag ttcacgggcc
     1441 agcggcgtgg ccagttccac catcggtgtg tacaggcatt ccagcacgtt ggggttggcc
     1501 ttgagagcca gtatcaggaa cttgccgagc tcccagtagc attcttcggt ctcggtgttc
     1561 tccagttgct cgggcacgcc gtaaagcgac cagtgccgct cggccggggg caaatagatc
     1621 ccgcggcggt cggtgtcgga ttcttcgttg tcgagcccat aggcccgcga accgctgatg
     1681 cagcggtaga tgacgaactg cgtgagatcc tggtcgttgc catcgtccgg cccgcgacgc
     1741 agaccttcct cttggtgctg tttgcggacc gagaagtcct tgcggtgcag ggtcgcttcg
     1801 ccgccgttga cgaatcggac gcggtatgcg tgggtgctgt cggccgggga gcggaggacg
     1861 atcccaaccg ccccgcgcgg ttgccccggc tggccggccg acgcggcgat ctccacccgc
     1921 gtcacgatct gcgtgccgac agggagaatg ggcggtgggt tggatctggt gccattcatg
     1981 gtgcccaggt atccgtcgca ggatgtccgt gaggcgatcg atcatcgtct gatcgcccac
     2041 atgtgtgtcg gcggctcatc cttggagaag cattctacga gagagtagtg ggttggtcac
     2101 acctgtgcgg gagagtcgca tgcggagacc acatccactt cgcaagacac catcgtgacc
     2161 ccattatcgc ccggcccgga atcccttttt gctcggcccg gaatccctct tcgctcggcc
     2221 cggaatccct cttcgctcgg cccggaatcc ct
//
LOCUS       DAOTPW010000422         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_37654_length_2245_cov_4.734703, whole genome shotgun sequence.
ACCESSION   DAOTPW010000422 DAOTPW010000000
VERSION     DAOTPW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37654_length_2245_cov_4.734703"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="P5316_21720"
     CDS             complement(<1..238)
                     /locus_tag="P5316_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87633.1"
                     /translation="MSDATMRAAIKTRLTAMGTAIGRVHDYERWTANTRDFLSLFQDP
                     ATKKIFGWEIMRTGFRMQKATMAKWKLIHRYMIRG"
     gene            complement(235..738)
                     /locus_tag="P5316_21725"
     CDS             complement(235..738)
                     /locus_tag="P5316_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87634.1"
                     /translation="MRISSKLIWKGTLFTGRPSETVRKQQVAAMHEATQFTSVKVKER
                     TPEGVMGEENAGLADSIQPEVRKPSTGVIGIVGTPSLYGLVVEKGRRPGRGRPPQGVL
                     LRWIEVKMGVSAETAEKIEPAVRWKIAKKGTEGAHMFEKTLDEDWPDIERIFQRYGVA
                     IAKGLSR"
     gene            complement(746..1387)
                     /locus_tag="P5316_21730"
     CDS             complement(746..1387)
                     /locus_tag="P5316_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87635.1"
                     /translation="MSLIDLVKSKVKDDSGRLTDDDDYSPAVAAALAKYSKDRPGELV
                     DDVNGAGTHDVDLPDGWVEGFSRVSSVEYPVGLVPEAYLPERDWRIYRGPDGPVLRLL
                     REVPSIGSPVRVTITVPRVEADIVSGDLDAVGCLAASFCCETLANLFAQTSDPTIAAD
                     VVNYRTKSGEFAARGKRLRQLYLDHLGIDADGGFPAASVKASAPETDRVRLTH"
     gene            complement(1400..>2245)
                     /locus_tag="P5316_21735"
     CDS             complement(1400..>2245)
                     /locus_tag="P5316_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87636.1"
                     /translation="ASAYSVKQKLFGFGAVAGEALATGDVVCIKDADGLAYKADADAV
                     ALRPAVGIIGLGAAAAGDPVEIITHGILRGWASLSEGQNAYLSTTAGAVTQSAPAYAQ
                     KIGFAVSATEYLFSFQEYLDTSALSVLGVLTGATPLIFEGATADAYETTISVTDPTDD
                     RTITIPNRSGQVQLASAASVLTPGEAVTLTVGLSNLYTLAINDNEDTTITFSGAGTAG
                     DEITIVIATGAGTTGDEVVTFHATLVSSTGTLTCANTANRFYVIRFISDGSHWYEVSR
                     TAIQT"
BASE COUNT          413 a          734 c          637 g          461 t
ORIGIN      
        1 agccgcggat catgtaccga tggatcagct tccacttggc catcgtggcc ttctgcatcc
       61 ggaaccccgt ccgcatgatc tcccagccga agatcttctt cgtggccgga tcctggaaga
      121 gcgaaagaaa atcccttgtg tttgccgtcc agcgttcgta atcgtgcacc cggccgatgg
      181 ccgtccccat ggcagtcaaa cgcgttttga tagccgctcg catcgtcgca tcgctcatct
      241 gctcaacccc ttagcaatcg ctaccccgta acgctgaaag atcctctcga tgtcgggcca
      301 gtcttcatcc agcgtcttct cgaacatgtg tgcgccctcg gtgccctttt tcgctatctt
      361 ccatcgcacg gccggctcga tcttttcggc ggtttcagcg gagaccccca tctttacctc
      421 gatccagcgc agcaggacac cctgcggcgg ccgacctctt cccgggcgac ggcccttttc
      481 aacgacgagg ccataaaggc tcggtgtccc tacgataccg atgacgccgg tgcttggctt
      541 gcgcacctcc ggctggatcg aatcagccag gcccgcattc tcctcgccca ttaccccttc
      601 gggagtccgc tctttgacct tgacgctggt aaattgcgtg gcctcgtgca tggccgccac
      661 ttgctgcttt cgtaccgttt ccgacggccg gccggtaaag agggtgccct tccagatgag
      721 cttgctcgat atcctcatac tgccctcaat gggtaagccg cacccggtca gtctccggag
      781 cggaggcctt caccgacgcg gcaggaaacc cgccgtccgc gtcgattcca aggtgatcca
      841 gatagagctg cctgagccgt ttcccccggg ccgcaaactc tccgcttttg gtcctgtaat
      901 tcaccacgtc ggcggcaatg gtaggatcgc tcgtttgggc gaacagattc gccagggtct
      961 cgcagcagaa ggaagccgcg aggcagccta cggcgtcaag atctccggag acgatgtccg
     1021 cctcgaccct ggggaccgtg atcgttactc tgacagggga cccgattgag ggcacctcgc
     1081 gcagcaaccg caggaccgga ccgtccgggc ctcggtagat ccgccagtca cgctccggca
     1141 aatatgcctc tggaaccagg cccaccgggt attccacaga actgacccgg ctgaaccctt
     1201 ccacccatcc gtcgggaagg tcaacgtcgt gggtcccggc gccgttcacg tcgtccacca
     1261 actcgccggg cctgtccttg gagtacttgg ccagggcagc ggcgacggca ggcgagtagt
     1321 cgtcgtcatc cgtcagccgc ccggaatcat ccttcacttt gctcttgacc aggtcgatca
     1381 ggctcaactt ttcctcccct tacgtctgga tcgccgtcct ggagacttcg taccaatggc
     1441 tgccatcaga aatgaacctg atcacataga accggttggc ggtattcgcg caggtcagtg
     1501 tgccggttga agagacgagc gtggcatgaa aggtcacgac ttcatctccc gttgttccgg
     1561 cacccgtggc aatcacgatg gtgatctcgt cccctgccgt cccggctccc gagaaggtaa
     1621 tcgtcgtatc ctcgttgtcg ttgatcgcca gcgtatagag attcgacaac cccacggtga
     1681 gcgtgaccgc ctcgcctggc gtgaggacgc ttgccgccga ggcgagttga acctgcccgg
     1741 acctgttggg gatcgtgatc gtccggtcgt ccgttggatc ggtcacggag attgtcgtct
     1801 cataggcgtc ggctgtcgct ccctcaaaaa tcaagggagt agccccggtc aagacaccca
     1861 ggacggaaag agcggaggta tccaggtact cctgaaaact gaacaggtac tcggtggcgg
     1921 atacggcaaa gccgatcttt tgggcatagg cgggagcgct ttgggtgacc gccccggccg
     1981 ttgtggaaag ataggcgttc tgtccctcgg agagcgaagc ccagccgcgc agaataccgt
     2041 gcgtgatgat ctccacggga tcccctgccg ctgcagcccc caggccgata attcccaccg
     2101 caggacgcag ggcgaccgca tcggcatctg ccttataggc caatccgtcg gcatccttga
     2161 tgcagaccac gtcgccggtt gcaagagcct cgccggccac ggcaccgaaa ccaaagagct
     2221 tctgtttgac gctgtaggca gaggc
//
LOCUS       DAOTPW010000423         2223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_38148_length_2223_cov_5.844096, whole genome shotgun sequence.
ACCESSION   DAOTPW010000423 DAOTPW010000000
VERSION     DAOTPW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38148_length_2223_cov_5.844096"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="P5316_21740"
     CDS             <1..651
                     /locus_tag="P5316_21740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR87637.1"
                     /translation="IAHHFNNLLGGALTALDFVQDSFEVETLRRGLRTALTALSRVSE
                     ITLALLTFAEGDRTDSPTDDVVDTVRQFVAGRRKEWARNRISVETELRPLRAKLPAKR
                     LMTLLRILTTNACEAMVGGGTLRIELSKGPGMTVVLRVRDTGAGVPEEQIRHVFEPFF
                     TTKRAVNPEDPEHIGLGLAVAYGLVKGMGGTITLEPGDPKGTVCTVCLPVHLEASG"
     gene            1169..2077
                     /locus_tag="P5316_21745"
     CDS             1169..2077
                     /locus_tag="P5316_21745"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HRR87638.1"
                     /translation="MIETQPRRRRGRHIKIIIGLIVLAGVLVHVREPWARKTQPAADA
                     SESATSSQRNAAFAALVPLHAHLGESIEERAARDPIHFFEDAVEYYDSAVRDYTCVFT
                     KQERIGGKLGPEQVTNAMFREKPFSVRLEWTRNADKCDRVLYVADRWVENNQQMAVVE
                     PGAVARLFVSHVMRPIHGKDAMRSSRRTIDQFGLRNTLCLALKYAKLSREHGTLDFKY
                     RGKSQVDGRETLLFERRLPYTGEGGEWPDRLLLIHLDKEMLVPTLCLCYADDEQQVLL
                     GKYMTTNIKLNVNLPDSVFTKEGMGL"
BASE COUNT          423 a          715 c          660 g          425 t
ORIGIN      
        1 atcgctcacc atttcaacaa ccttctcggc ggcgccctga ccgccctcga cttcgttcag
       61 gattcctttg aggtggagac gcttcgccgc ggtcttcgca ccgccttgac ggcgctcagc
      121 cgcgtcagcg aaataactct tgccctcctt acctttgccg agggagaccg caccgacagc
      181 ccgacggacg acgtcgtcga caccgtccgg cagtttgtgg ccggccgacg caaggaatgg
      241 gcccgaaacc gcatttccgt cgagaccgaa ctgcgacctc tcagggcgaa gcttccagcg
      301 aaacgcctga tgactctctt gcgaatcctc acgaccaatg cctgcgaggc catggtcggc
      361 ggagggacgc tccgtatcga gctcagcaaa ggacccggca tgactgtggt tcttcgcgtc
      421 cgtgataccg gagcgggtgt gcccgaggag caaatccgcc acgtcttcga acccttcttc
      481 acgaccaaga gggctgtcaa tccggaagac ccggagcata tcggcctggg cctggccgtt
      541 gcctatggat tggtgaaagg catgggcggc acaattactc tggagcccgg cgatccgaaa
      601 ggcacggtgt gtacggtctg cctgcctgtt cacctggaag cctccggttg acgcgtttct
      661 gagacactgc cccgccggcg atccggtgtc ccctcgccat ctcccccggt ttgccggttc
      721 caggcaatgc cgcatcctcg cattagtccc ccctgtataa ggggatagtg aaaacggtct
      781 gctggtctgc gagccgctct tattgttcgc ttggatcacc gcgcttgttg aggaccggtc
      841 cggccgttac cataaggttc attcggcaca cccgggtcgt ggacccgctt gccgggttgg
      901 aggtcgtccc cctgaatccg atgctccgca ggcgcgatgg cgggcgggtc ccaggggctg
      961 caaaactcgg ccgtatgagt attgattgta aagccccccc gatcaggcag ccgatatccg
     1021 aggtggatgg accgagcgcg gtcgagcccg cgacgctgtg agatgaagat ccattcgaca
     1081 ggcgccgatt cagcaggacc ggcgccggcc gcgggaatgc caagagcctt ccctgtcggt
     1141 tcacgtgaga ggctttcagg atcagtccat gatcgaaact caaccccgtc gccgccgcgg
     1201 acgtcacatc aagatcatca tcggtctcat cgtgctcgcc ggagtgcttg tccatgtgcg
     1261 cgagccttgg gctcgcaaga cgcagccggc cgccgacgcg tccgagtcgg caacatcctc
     1321 tcagcgcaac gcggccttcg cggccctcgt tcccttgcat gcccacttgg gcgagtccat
     1381 cgaggagcga gccgctcgag acccgattca tttcttcgaa gatgccgtcg agtactacga
     1441 tagtgccgtt cgcgattaca cctgcgtctt caccaaacag gagaggatcg gcggcaagct
     1501 cggcccggag caggtaacca acgccatgtt ccgcgagaaa cccttcagcg tgcggctgga
     1561 gtggaccagg aacgccgaca agtgcgatcg cgtcctctac gtcgccgacc gctgggtcga
     1621 gaacaatcag cagatggccg tcgttgagcc gggggcggtc gccaggctct tcgtcagcca
     1681 tgtgatgcgc cccatccatg gcaaggatgc gatgaggtca tcccgccgga cgattgacca
     1741 gtttggattg cgaaacacgc tctgcctggc actcaagtac gccaagcttt ccagggaaca
     1801 tgggacgctg gatttcaagt acaggggcaa gagccaggtc gacggccggg aaacgttgct
     1861 cttcgagcgg cgactgccct ataccggcga aggcggtgag tggcccgatc gcctcctgct
     1921 catccatttg gacaaggaga tgctcgtccc aaccctgtgc ctgtgctacg cggacgacga
     1981 gcagcaggtt ctcctcggca agtacatgac caccaacatc aaactcaatg tgaatctgcc
     2041 ggattcggtg ttcacgaagg agggcatggg actctagcgg cgagcgcaaa acgctcctcc
     2101 gtatgctcct caacacttcc acttaacgat cgacggccgg ccccgagggg ccggccgttg
     2161 tgtttcccga accgctgaac gctttccccc gtgcggccat cgccccaagc tggtatcatg
     2221 aaa
//
LOCUS       DAOTPW010000424         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_38379_length_2213_cov_5.034291, whole genome shotgun sequence.
ACCESSION   DAOTPW010000424 DAOTPW010000000
VERSION     DAOTPW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38379_length_2213_cov_5.034291"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="P5316_21750"
     CDS             complement(<1..1272)
                     /locus_tag="P5316_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87639.1"
                     /translation="MTVSRAVFDRSLTQANRESCGKRAWYLISFGGIAAVLALSLATS
                     RICSAQVFPGATWQIKTPAEVGMDVKRLDAFRDYLGGRGCVVRHGYMVYTWGDQTRRQ
                     DVASACKPVIAHFLFKALEEGKLASLDEPVVRWEPCLNDINPDLDFKDRLINFRHMAT
                     QTSCYGVREKPGTAFDYNDWQMALFWDTLFLKVYGITYDTVDAQALHPRLTDLMQCQD
                     DPTFTFFGTGNRPGRFGISVRDFARFGLLYLHKGNWNGKQLLSKANATLAVTSPLPNS
                     IPRTDARPAQICPGQRSLGSQKVPDDQTDHLGSYSYLWWINGVDRDGHRHWPDAPLDT
                     FGAFGHKNGQRAVVVIPSLDLVVSWNDTNLGERSGNPVNEAIRLLVQSTKAGPIIADH
                     QHPAWLEYQGGGPCYMCGPGDPEGFLYRGTRN"
     gene            complement(1417..>2213)
                     /locus_tag="P5316_21755"
     CDS             complement(1417..>2213)
                     /locus_tag="P5316_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87640.1"
                     /translation="GNCGRLVRFMEATRFNETSPHDELAGCDTDYVLANPGEVYIGYG
                     DGGDAFQLFMVPGNYAVKWYDPTTGLWVDQATRVVLSAGSQLFAKPGTFAQDAALYVV
                     RTVPARADNPFPPSGTLRVPRDVRLSWTAGAGAVSHDVYFGTTNPGAFRGNQTDTFFD
                     PGLLDPLTTYFWRIDEVNEMGATTGTVWSFTTQGVAADMDGDGDVDQEDFGLFQACLT
                     VMGDPISPECLTADFDRNGLVNQSDHTVFIRCLSGPGVPADPDCAS"
BASE COUNT          446 a          676 c          663 g          428 t
ORIGIN      
        1 attgcgcgtg ccccggtaca agaagccctc cggatctccg ggaccacaca tgtagcaagg
       61 accgccgccc tggtactcaa gccatgccgg gtgctgatga tccgcaatga tggggcccgc
      121 tttcgtcgac tgtacaagca gccgaattgc ttcgttcacg gggttgcctg acctctcccc
      181 gaggttcgta tcattccagg acactacgag atcgagactg gggatgacga ccaccgcccg
      241 ctgaccgttt ttgtggccga aggctccgaa cgtgtcgagc ggggcgtcgg gccagtgccg
      301 atggccatca cggtcgactc cattaatcca ccagagatag ctgtaactgc ccagatggtc
      361 ggtttggtca tccggcacct tctgcgatcc gagggaacgc tggccggggc agatctgggc
      421 gggccgagca tcggtccgag ggatcgagtt gggaagagga cttgtcacgg ccagggtggc
      481 attggccttg ctcaggagct gtttcccgtt ccagtttcct ttgtgaagat acaacagccc
      541 aaagcgtgcg aagtctcgca ccgaaatgcc gaaccgtccc ggccgattcc ccgtcccgaa
      601 gaaggtgaag gtcgggtcat cctggcactg catcagatct gtcaacctgg gatgcagagc
      661 ctgggcgtca acggtatcgt aggtaatccc atagaccttg aggaacaacg tgtcccagaa
      721 aagggccatc tgccagtcgt tatagtcaaa ggcggtgccc ggcttttccc ggacgccgta
      781 acacgaagtc tgagtagcca tgtggcggaa gttgatgagc cgatccttga agtcgaggtc
      841 cgggttgata tcgttcaggc acggttccca cctgactacg ggctcgtcga gactggccag
      901 cttaccctcc tccaaggcct tgaagagaaa atgggcaata acgggcttgc aggccgaagc
      961 gacgtcctgc cggcgagtct ggtctcccca cgtatagacc atgtagccgt ggcgaacgac
     1021 gcagccccgc ccgccgagat agtctcgaaa ggcatccagc ctcttgacat ccatgccgac
     1081 ctcggccggt gtcttgatct gccaggtggc acccggaaag acctgggctg agcagattcg
     1141 gctcgttgcc aaggacaggg ccaagacggc cgcgatgcct ccgaacgaga tcaggtacca
     1201 tgctcgtttg ccgcagctct cgcggttggc ctgagtgagg ctgcggtcga atacggctct
     1261 ggaaacggtc atattactcc cttgtcgccc gctggttgtc ggggctttct tcggagcgga
     1321 ccactcggac aggtcattgt gcgcgctttg acgcaagacc caggccgaag atgcaactcc
     1381 gcaagcctgc tcgatcagcc gctacccaag aatccctcag cttgcacaat ccgggtcagc
     1441 gggtactcca ggcccgctca agcaccggat gaacacagtg tggtcggact gattcacgag
     1501 tccgttccgg tcaaagtccg cagtcaggca ctccggactg atggggtcgc ccatgacggt
     1561 aaggcaggcc tgaaacaggc cgaaatcctc ctggtccacg tcgccgtcac cgtccatgtc
     1621 ggcggccact ccctgagtgg tgaagctcca taccgtgcct gtcgtcgcgc ccatttcgtt
     1681 cacttcgtct attcgccaga aataagtcgt gaggggatca agcaatccgg gatcgaaaaa
     1741 ggtgtcggtc tggttgccgc ggaaagcacc tggattcgtc gttccgaagt aaacgtcgtg
     1801 cgagaccgcg ccggcgccgg ccgtccagga gagccggacg tctcgcggaa ctcgcaacgt
     1861 ccccgacggc gggaacgggt tatcggctcg agcgggaacc gtacgaacga catacaacgc
     1921 cgcgtcctgc gcgaaggttc ccggtttggc gaaaagctgc gaacccgccg acaacacaac
     1981 ccgtgttgcc tggtcgaccc acagtcccgt cgtgggatcg taccatttga cggcatagtt
     2041 gccgggcacc atgaacagtt gaaacgcgtc gcctccgtcg ccgtaaccga tgtacacctc
     2101 tcccgggttg gccaatacgt aatccgtgtc gcagccggcc agttcgtcat ggggcgaggt
     2161 ctcattgaag cgcgttgcct ccatgaatcg aaccagtcgg ccgcagtttc caa
//
LOCUS       DAOTPW010000425         2156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_39744_length_2156_cov_5.771061, whole genome shotgun sequence.
ACCESSION   DAOTPW010000425 DAOTPW010000000
VERSION     DAOTPW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39744_length_2156_cov_5.771061"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="P5316_21760"
     CDS             <1..720
                     /locus_tag="P5316_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87641.1"
                     /translation="VPTDPPSGASLAQGPYSGFLARDASLSTGLYPATIDPWTTKRFC
                     HTRIDVWTNYIRVTWASKQLGVLWTATVPRRYHGAFKSLYLGNRGCAQPAYPLYLDTL
                     QLNGGLFTTSMETYGACCNHDTGVCVSTGTVQECNGLGGEFHLFRMCGDIDCYRNPPI
                     PFADADRDHDVDQNDFAAFQACFTGESGGILNPDCGLFDRDDGGAGDGDIDRDDLLAF
                     EACATGPGVPFDPSNPGDCVP"
     gene            complement(895..1929)
                     /locus_tag="P5316_21765"
     CDS             complement(895..1929)
                     /locus_tag="P5316_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87642.1"
                     /translation="MSFKNNLVVSFVHGFHNPADAANDKPVPALWEIRSDGERVHSYV
                     FPFDGEDFRKLPYPVFGELPAGRPVLEKRARFGFTGLARVGDKLFAGSWNGIYQIDLA
                     GKQVSRFISNRYSCYMHRIYADDQHIILAMPFKDMVVIMDHEGRIEDRFWIDRSLRVV
                     RDDRDSVDWRFMNKPWSGSTGFYHLNHVQKLDGKILLTSRNLGALIVADPRLDRCELR
                     TLNYHTTSCIHDGDYVDGRYWFTSIDGKVIVASEPPEHDVGLFNYDLQAEHFRLGEKE
                     RNWCRGIAVTDDRVYVTVDGRYGADLSFSVLELDKQCNILDERKFQWSAIGDTSKIRF
                     VTGFDIKVID"
     gene            complement(1926..>2156)
                     /locus_tag="P5316_21770"
     CDS             complement(1926..>2156)
                     /locus_tag="P5316_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87643.1"
                     /translation="RTDLARRYAYALFFRSNIRISWVTVLDLNVRSINIRSLAELGPG
                     QDAAMDAICRSILCDEPCEDPSSRHPGEHAPI"
BASE COUNT          414 a          639 c          672 g          431 t
ORIGIN      
        1 gtgcctaccg atcctccctc tggcgccagc ctggcgcaag gtccatattc cggcttcttg
       61 gcacgggacg cgagcctcag caccggcctt tatcccgcca cgatcgaccc gtggaccacc
      121 aagcggttct gtcacacgag gatcgacgtg tggaccaact atattcgcgt gacctgggcc
      181 tcgaagcaat tgggcgttct ctggactgcg acggtgccga gacgctacca tggggccttc
      241 aaatcgcttt acctcgggaa tcgcggctgc gcgcagccgg cttacccgct ctacctggat
      301 accctccagt tgaacggagg gttattcacg acgtccatgg aaacttacgg agcctgctgc
      361 aatcatgaca cgggggtttg cgtcagcacc ggcacggtgc aggagtgtaa cggcctgggc
      421 ggcgagttcc atcttttccg gatgtgcggc gacattgatt gttaccgtaa cccgccgatc
      481 ccatttgctg atgccgaccg cgaccacgac gtggatcaga acgactttgc cgcgttccag
      541 gcctgcttca ccggcgagtc aggcggtatc ctcaacccgg actgcggtct cttcgaccgc
      601 gacgacggcg gggcggggga cggcgacatc gaccgcgacg atctgctggc atttgaagcc
      661 tgtgctaccg ggccgggtgt tccgtttgat ccgagcaatc ccggggattg cgttccgtag
      721 agccgcagcg ggcgggtgcg agggcggcga ttctatcgtg cagatctgcg gactcagatg
      781 gtcctaggcg atccggcgcg agtgggggat ggctgcttcg ggcttcaagc tgacaatcag
      841 atccgcgacc attgccatcc ttgtcgcccg gtgacgccga acgtaacgca cgcctcagtc
      901 aatcaccttg atgtcgaacc cggtgacgaa ccggatcttc gaggtgtcgc cgatcgccga
      961 ccactggaac ttgcgctcgt cgagaatgtt gcactgtttg tcaagctcca gcacggagaa
     1021 actcaggtcg gctccgtatc ggccgtcgac ggtgacgtac acgcggtcgt cggtgacggc
     1081 aattccgcgg caccaattcc gctctttttc gccgaggcga aagtgctcgg cctggaggtc
     1141 gtagttgaaa aggccgacgt cgtgctcggg cggttcgctg gcgacgatga ccttgccgtc
     1201 gatgctggtg aaccagtagc gcccgtccac atagtccccg tcgtggatgc aactggtcgt
     1261 gtggtagttg agcgtacgga gctcacagcg atcgaggcgc gggtccgcga cgatcagagc
     1321 tccgaggttt ctcgacgtca gcaggatctt gccgtccagc ttctggacgt ggttcagatg
     1381 atagaatccc gtcgagccgg accagggctt gttcatgaac cgccagtcca ccgaatcgcg
     1441 atcgtcgcga acgacgcgga gcgaccgatc gatccagaaa cggtcctcga tgcggccttc
     1501 atggtccatg atgacgacca tgtccttgaa aggcattgcc agaatgatgt gttggtcatc
     1561 cgcgtagatg cgatgcatgt agcaactgta gcggttggag atgaaccggc tcacctgttt
     1621 gccggccaag tcgatctggt agatgccgtt ccatgaaccg gcgaacagct tgtcgccgac
     1681 acgggcaagg cccgtgaagc cgaaacgggc tctcttttcg agcaccggcc gtccggcggg
     1741 cagttcgccg aaaacggggt acggcagctt ccggaaatcc tcgccgtcaa acggaaacac
     1801 ataactgtgg acgcgctcgc cgtctgagcg gatctcccac agcgccggaa ccggcttgtc
     1861 gttggcggcg tcggccggat tgtgaaagcc gtgcacgaat gaaacgacga gattgttctt
     1921 gaagctcata ttggggcatg ttctccggga tgacgtgatg aagggtcctc gcaaggttcg
     1981 tcacacagaa tcgagcggca aatggcgtcc attgccgcat cctgccccgg acccaactcg
     2041 gccaggctgc ggatgttgat cgaacggaca ttcaggtcaa ggaccgtgac ccaggatatt
     2101 cgaatattgg accgaaagaa cagggcgtac gcgtaccgtc gggcgagatc cgtccg
//
LOCUS       DAOTPW010000426         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_40935_length_2108_cov_5.285923, whole genome shotgun sequence.
ACCESSION   DAOTPW010000426 DAOTPW010000000
VERSION     DAOTPW010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40935_length_2108_cov_5.285923"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="P5316_21775"
     CDS             <1..1506
                     /locus_tag="P5316_21775"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87644.1"
                     /translation="VWGQTFRATSVALAAADCFIAGSGTWDVDVVFRIRQNGPGGAQV
                     GPAKIAQATYQAVNTGIVGVSYAPGEVPLTPGSTYFIEMSPAAGSPAFAAYKFHSQAD
                     NGYPYGSAYKDGVLQSGVDLEMTIMEYKPWYSPAILNPGFEDHGGTLDDWRITLFDGK
                     GPDKPPHDNTNPYGPRTPFGDHFAGKITSWQSMDFTMGQIVEVSNYDPAAERVVCSLS
                     AYVQLHGHTGLTEEPLNVHQLWEIGWNNDGSVPASVNRCDHYRPLASIDGTYTGNDRT
                     NFYPLTAAGEIRGVIGLKYVALRVRLYNGAPREWSMSNIDNVSFVAAVPPPSPKLLVE
                     PRVIERVLWIGETATPDVFTVTNAGTGSLSYEISDDASWLEVSPTSGSSTGESDPITV
                     TYDLAGLPAAERTATITVVSQEAWNSPQTISVTFTTWTVGPDFDRDGDVDQFDFGIFQ
                     RCLSGAGVVQQDPLCEPTLLDADEDVDGDDLSIFLRCLSGPGLLAEPTCDD"
     gene            complement(1690..>2108)
                     /locus_tag="P5316_21780"
     CDS             complement(1690..>2108)
                     /locus_tag="P5316_21780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR87645.1"
                     /translation="TTRHDVVVTAMSLPDGDGLALTRELRLTNRCPVILTTEQPTAEE
                     AIEALRLRVTDVLIQPFDIADLMDLIQKSAERRLCYRHRRRRYRRLRAMVSRIIRERR
                     DLRQRMDLICRDLVHAYRNLAQKVAQSGVLTHDLKE"
BASE COUNT          386 a          645 c          667 g          410 t
ORIGIN      
        1 gtctggggac agacgtttcg tgcaaccagc gtagcgttgg ccgctgctga ttgtttcatc
       61 gccggctccg ggacatggga tgtcgacgtc gtcttccgta tccgtcagaa cggcccgggc
      121 ggggcacagg tcggccccgc caagattgcc caggccacgt atcaggcggt caacaccggc
      181 atcgtcggcg tcagctatgc tcccggcgag gtgccgctca cccccgggag cacctatttc
      241 atcgagatga gtccggcggc cgggtctccg gcgtttgcag cctacaagtt ccattctcag
      301 gccgacaacg gctaccccta cggatcagca tacaaagacg gcgtgctcca gtccggcgtg
      361 gatctcgaga tgacgatcat ggagtacaag ccgtggtact cgcctgccat cctcaacccc
      421 ggttttgagg atcatggcgg taccttggac gactggcgca tcacgttgtt cgacggcaaa
      481 ggccccgaca agccgcccca tgacaacacc aacccctacg gaccccgaac gccgttcgga
      541 gatcatttcg ccggcaaaat cacgagctgg cagtcgatgg attttacgat gggacagatc
      601 gtcgaggtga gcaactacga tccggcggcc gaacgcgtgg tttgctcgct gtcggcgtac
      661 gttcagctcc acggccacac cgggctcacg gaagagcctc tcaacgttca tcagctctgg
      721 gagatcggct ggaacaatga cgggtcggtg cccgcgagcg tcaaccggtg cgaccactac
      781 cggccccttg cttcgattga cggcacctac acgggcaatg atcgcaccaa tttctacccc
      841 ctcacggcgg ccggcgagat tcgcggtgtt atcggcctta agtacgtcgc gcttcgcgtt
      901 cgcctctaca acggcgcgcc tcgcgaatgg tcgatgagca acatcgacaa tgtcagcttc
      961 gtcgccgccg tgccgccgcc gagtcccaag ctcctggtcg agccgcgagt cattgagcgg
     1021 gttttgtgga tcggcgaaac agccactccc gacgtcttca ccgtgacgaa cgccggaacg
     1081 ggttcgctgt cctatgagat cagcgatgat gcgtcgtggc tggaggtctc tcccacgtcg
     1141 ggctcatcaa ccggcgaatc cgatcccatc accgtcacct acgacctcgc gggcttgccg
     1201 gcggccgaac ggacggcaac aatcaccgtc gtatcgcagg aggcctggaa cagtcctcag
     1261 accatttccg tcactttcac aacgtggacg gtcggtcctg attttgaccg cgacggcgac
     1321 gtggaccagt ttgatttcgg gatatttcag cgatgcctgt cgggggccgg cgtcgtgcag
     1381 caggatccac tctgcgaacc gactctactg gacgctgatg aggatgtgga cggcgacgac
     1441 ctgagtattt ttctgcgatg cctgagcggg ccgggcctgt tggcagaacc gacctgcgat
     1501 gattgaggcc ttgagtgctc cgggaatccc gattctcgcg ttttgcggtg cgatcaggac
     1561 cgtttgccgg gacggatgtg gaaccttgcc gccgtccggg gcgtcaccgg gcggagggaa
     1621 cgttctttgt gagtctacgg ggaggagctt ccggaggcgg gtcattcacg gaccggtagg
     1681 ggagggtctc tattctttca ggtcgtgcgt aagcacgccg gactgtgcca ctttctgagc
     1741 caggttgcga taggcatgga ccaggtcgcg acagatcaga tccatccgct ggcggaggtc
     1801 gcgacgttcg cgaatgatgc gcgagaccat ggcacgcagc cgtcgatatc gccgccggcg
     1861 gtggcggtaa caaaggcgcc gctcggctga cttctggatc aggtccatca ggtcggcaat
     1921 atcaaaaggc tggatgagca cgtcagtgac tctgaggcgg agggcttcga tggcctcctc
     1981 ggccgtgggc tgctcggtgg taaggatcac cggacaacgg ttggtcaggc gcagctcccg
     2041 ggtgagggca aggccgtctc catcgggcag ggacatcgcg gtcaccacca cgtcatgccg
     2101 cgtggtca
//
LOCUS       DAOTPW010000427         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_41387_length_2090_cov_5.667322, whole genome shotgun sequence.
ACCESSION   DAOTPW010000427 DAOTPW010000000
VERSION     DAOTPW010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41387_length_2090_cov_5.667322"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            600..1880
                     /locus_tag="P5316_21785"
     CDS             600..1880
                     /locus_tag="P5316_21785"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR87646.1"
                     /translation="MPARPARKNLMRRRAFLKQLAALAGTSALGGCAGAPSAGRPKPA
                     GVLGSNDRIRLGVIGCGNLGFAHHIPKLMELSKDPRMNVELAAVCDIYEPRKQRAKAR
                     TGAALFHDYRKMLDRRDIHGVVIVTPDHWHAKMAIDAMEAGKDVHVEKPMTLYWEEAR
                     QVYETAVRTRRVVQVGAEGTSRDVYWQANRLIREGKIGKPVWATGGVYRNDPAGDWNW
                     PMDPDCSPKNLDWDAFLGPAPKRPFDPERYFRYRKYWDYSGGVAHDLIAHTLSALQIC
                     MGAEFPKRVSAAGGIYVHHDRETPDTFSMQVEYPSQYLATLFCTQTTESGVSFAIRGE
                     KGTITFSPNKEIPGKVYVKPEPPFVKEMQELTVTEQPRPDHDVNFIECMRTRAETACP
                     PLTGYKIMVALALAVRSYREGKMFTFDPVRQEIV"
BASE COUNT          454 a          719 c          588 g          329 t
ORIGIN      
        1 ggaatccttt ccgggccgca tcccctgcgg aatcctttcc gcattcagcg cccatcacga
       61 tggcaatcct ccatcagcag ctagggcagg tcacagccct accaagtcac accgaccaag
      121 cacagcgtcg acctgcctgg cattcggaac ggagcccccc tccggccgat cgccaagcgc
      181 cgaccgcctc cccgcgccaa acgaacccaa caaaccaatg atcgaggaac tgtaaccgat
      241 gtgccgacag ggtgttatgc aatacaagat ggtaaatacc cgcggtcaaa gctctcctcg
      301 attcccggcc ggctgccgct tggaacttaa cccaaaacct ctgtaccagc ggcttccagc
      361 ccgtcgggat acgcttccag ccacaggcaa ccaatccccg ctgggccact cccctcaagt
      421 caaggtggca gccaacggtg cggcgccctg tctgccctgg cagcaatccg ccaactctgt
      481 ccgactcccc cgaaaccccg accgccattc ctacccacgt gccgcgatct caggtacgat
      541 gcttccccgt gtgaatcctt caaccgtcga gccatccaac ttactacagg agtccatcaa
      601 tgcccgccag gcccgcccgt aagaacctca tgcgccgccg agcatttctc aagcaattgg
      661 ccgccttggc cggaacctcc gccctcggcg gctgcgcggg cgcgccttcc gcaggtcgcc
      721 ccaagcccgc cggggtcctc ggctccaacg atcgcatccg ccttggcgtc atcggctgcg
      781 gcaatctcgg cttcgctcac cacattccca aactgatgga attgagcaaa gatcccagga
      841 tgaacgtcga actggcggct gtctgcgata tctacgagcc gcgcaaacag cgggccaagg
      901 ccagaaccgg cgccgccctg ttccacgact accgtaagat gctcgaccgg cgggacattc
      961 acggggtggt catcgtcacg cccgaccact ggcacgcaaa aatggccatc gacgcgatgg
     1021 aggccggcaa ggacgttcac gtcgagaagc ccatgaccct ctactgggaa gaggccagac
     1081 aggtgtacga aaccgccgtg cgaacccgcc gcgtggtgca ggtcggagcc gagggaacgt
     1141 cacgcgacgt ctactggcag gccaacaggc tgatccgcga aggtaaaatc ggcaagccgg
     1201 tttgggcgac cggcggcgtc tatcgcaacg acccggccgg cgactggaac tggccgatgg
     1261 accctgattg ctcgccgaag aacctcgact gggatgcgtt tctcgggccg gcgcccaaac
     1321 ggccattcga cccggaacgc tacttccggt accgcaagta ctgggactac tcgggcgggg
     1381 tggcccatga cctgatcgca cacacactct ctgctttgca gatctgcatg ggggcggagt
     1441 tccccaagcg agtcagcgcc gccggcggga tctacgttca ccacgaccgc gaaacgcccg
     1501 acaccttcag catgcaggtc gagtacccga gccagtatct ggccacgctg ttctgcacgc
     1561 agacgaccga gagcggcgtc agctttgcca tccgcggcga gaagggaacc atcaccttca
     1621 gcccgaacaa ggagataccg ggcaaggtgt acgtcaagcc cgagccgccg ttcgtcaagg
     1681 aaatgcagga actgaccgtc accgagcagc cgcgccccga ccacgacgtc aacttcatcg
     1741 agtgcatgcg cacccgggcc gagaccgcct gcccgccgct caccggctac aaaatcatgg
     1801 tcgccctggc cctggcggtg cgaagctatc gcgaggggaa gatgtttacg ttcgatccgg
     1861 tcaggcagga aattgtctga ataagctgtc ggctcccagc agtcagcaat cagccgtgta
     1921 aggcgtcaag agtgtattag aattaaaaag aatctaagcc tggaaactgc ctagggcagg
     1981 tcggagcccc cggatcagta gggcacggtc gaagcggcca gatcacgtag ggcatggtcg
     2041 aagcggccaa aggccgcgca gacctgccgc aaccatcccc gtaggacatg
//
LOCUS       DAOTPW010000428         2038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_42842_length_2038_cov_4.262733, whole genome shotgun sequence.
ACCESSION   DAOTPW010000428 DAOTPW010000000
VERSION     DAOTPW010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42842_length_2038_cov_4.262733"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..970)
                     /locus_tag="P5316_21790"
     CDS             complement(260..970)
                     /locus_tag="P5316_21790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR87647.1"
                     /translation="MLESTPETQRIMVVDDSAYTLRIVQHTLEGAGYTVSTAISGPEA
                     LKLITRYGLPHLAIVDYHMPEMSGFEFCRTIHEFSDVPVIMLTAVNTEETIVEGLEQH
                     AEDYMVKPFNPTELVARVRRVLARMEDYTYTLDSTTRIDDRLLINFPLREALVDGQPV
                     SLTPTETKLLYILVRNAGRIVTTDFLLGRIWPLQEAREDRLHVHVHRLRGKIERDPNN
                     PDYIVAERGVGYSFRSGA"
     gene            1197..1553
                     /locus_tag="P5316_21795"
     CDS             1197..1553
                     /locus_tag="P5316_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRR87648.1"
                     /translation="MLAEWQYIGIFVALSPIFPLAPVVLNRLLGPHRPNPIKQQTYEC
                     GIETVGDTWIQFKVQYYIYALVFVVFDIEAVFLFPIAAAFGQLDLFAIGAAVLFIFLL
                     SDGLIYAWSRGVLEWV"
     gene            1649..>2038
                     /locus_tag="P5316_21800"
     CDS             1649..>2038
                     /locus_tag="P5316_21800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRR87649.1"
                     /translation="MNILDLLKSLLSLSLGDFIRRNFGTGPGTELLIDLGGVVILSTF
                     CLLIVIFLIWLTRKIVARMQDRIGPNRVGGRFGLLQTVADVMKLLSKEVIHPAGADWV
                     AFNAAPLLIVISALLMWAVMPFAPGVIG"
BASE COUNT          364 a          635 c          606 g          433 t
ORIGIN      
        1 cgatctcccg tgccaattca ggaaccatcg gcaccatcgg cgttaacaca aaaatgccgt
       61 cgaggtcaag gatgcggaat gtatctccag gctcaatgtt atagcgttca cgcaactcgg
      121 ccggtagagt gagatttcct cgctggcgaa cctgtacggt ggtagcttcc atatcgataa
      181 cgctccttag aatcgacgtc agaatttcag ccacatctga atttctgcat cacgattcta
      241 gcagagcgcc gcataggcat caagcccccg accggaaact ataccccacc ccccgctcgg
      301 caacaatata gtcggggttg ttggggtcgc gctcgatttt gccccgcagc cggtggacgt
      361 ggacgtgcag ccggtcttcc cgggcctctt gcagcggcca gatgcggccg agcaggaagt
      421 cggtcgtcac aatgcggccg gcgttgcgca ccaggatgta gagcagcttc gtctccgtcg
      481 gcgtcagcga caccggctgg ccgtccacca gcgcctcgcg cagcgggaag ttgatcagca
      541 gccggtcgtc gatgcgcgtc gtcgagtcga gggtgtaggt gtagtcctcc atgcgcgcca
      601 gcacgcggcg cacgcgggcc accagttccg tcgggttgaa cggcttgacc atgtagtcct
      661 cggcgtgctg ctctaacccc tcgacgatgg tctcctccgt gttgacggcc gtcagcatga
      721 tcaccggcac atcgctgaac tcgtggatcg tgcggcagaa ctcgaagccg ctcatctccg
      781 gcatgtggta atcgacaatg gccaggtggg gcaggccata gcgggtgatg agcttcagcg
      841 cctccgggcc ggagatggcc gtcgagacgg tgtagcccgc cccttccagc gtgtgctgga
      901 cgatgcgcag ggtgtaggcg ctgtcgtcaa cgaccatgat gcgttgtgtt tcaggagtcg
      961 attcgagcat ggcgggattt gtccgaatgg tgttgtcgcc caagagcgtg atggcgctta
     1021 tggtaaacca agtcgccgcc tttggccaac ctgagccgcc ctattcttag gaagcgtccc
     1081 gccgccgcgg ccagagatga gacgcgcatt agcttgcgct attccccgcc tgtgctataa
     1141 aagaaacgaa tttcacaatc acgctccgca agcttgaatg aggaagagag atcaccgtgc
     1201 tggctgaatg gcaatacatc ggcattttcg tggccctgtc gcccatattc ccgctggccc
     1261 ccgtcgtgct caaccgcctg ctggggccgc accggccgaa cccgatcaag cagcagacgt
     1321 atgagtgcgg catcgagacg gtcggcgaca cctggattca gttcaaggtt cagtattaca
     1381 tctacgcgct cgtcttcgtc gtcttcgaca tcgaagcggt gttcttgttc cccatcgccg
     1441 cggcctttgg ccagctggac ctgttcgcca tcggcgcggc ggttctgttc atcttcttgc
     1501 tgtccgacgg ccttatctat gcctggagcc gcggcgttct ggagtgggtc tagctgccca
     1561 cagttccgac attgacagga ggaatagccc gcatcccagg cccaactggc ctgccgctgt
     1621 cgcctgagat gccggaggcg caactgccat gaacattctc gacctcttga agagtcttct
     1681 gtcccttagt ctgggcgatt tcatccgccg caactttggg actgggccgg gcacggagtt
     1741 gctgatcgat ctcggcggcg tggtcatcct ctcgaccttc tgcctgctga tcgtcatctt
     1801 cctgatctgg ctgacgcgca agatcgtcgc ccgcatgcaa gaccgcattg gccccaaccg
     1861 cgtgggcggc cgcttcggcc tgctccagac tgtggccgac gtcatgaagc tgctgagcaa
     1921 ggaagtcatc cacccggccg gagccgactg ggtggccttc aacgccgcgc cactgctcat
     1981 tgtcataagc gcgctgctca tgtgggcggt tatgcccttc gcccccgggg tcatcggc
//
LOCUS       DAOTPW010000429         2030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_43060_length_2030_cov_5.773165, whole genome shotgun sequence.
ACCESSION   DAOTPW010000429 DAOTPW010000000
VERSION     DAOTPW010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43060_length_2030_cov_5.773165"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="P5316_21805"
     CDS             <1..865
                     /locus_tag="P5316_21805"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRR87650.1"
                     /translation="VFGPTPVVLALRLKWLLAVHGVSLSVWQAVKVTFAGNFIIGTTP
                     LGTHGGDAMKAYYIARDTPYKHEAVTTVFVDRVIGVISLVGLAGVVCLANWRDPAFSK
                     WGQIIGLTVLVLLVGGAVYFSGRLRRLLRIDRFLLYLPLGSHIHRIDQAALEFRNRPG
                     RVAGGLVLTVVLQVIAIISHFLIGWGLNMVGEDPWGALPVYLAFVPICFLAGALPIGA
                     MELTYVELFARSAGLGTPETATFLSLLSRLIQLLWAMPGVLVVLKAGRPQPITDSEPA
                     DAAVASEGRPH"
     gene            1345..>2030
                     /locus_tag="P5316_21810"
     CDS             1345..>2030
                     /locus_tag="P5316_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87651.1"
                     /translation="MKRHSRSCLGLVLIAGLFMCVPFLVGQGCLPDSGRPTPGDSLPV
                     VNVIAPSVNRTAVAGDTITVLYDVLNPPVGSAGAIQVWGFYDRDGVYDGDEQTFASSL
                     ASGQGKFTSFNTSGISPGPLYIGIAAENAAGKTVDYAPARITLSATAAVTFSAPDGDL
                     TVGPGATIPIVFSAGSGVTNFTWSLFYDTDGAFNGDEFVIETGANTTSSVVSREWAIP
                     LDTPPGTYYIG"
BASE COUNT          317 a          567 c          677 g          469 t
ORIGIN      
        1 ggtgttcggt cctactcctg tggttctggc cttgcgcctc aagtggcttc tggccgtgca
       61 tggcgtgtcc ttgtccgtgt ggcaggcggt caaggtgacg tttgcaggga acttcattat
      121 tggcacgacg cctttgggga ctcatggcgg tgacgcgatg aaggcctatt acatcgcccg
      181 cgacacgcct tacaagcacg aggcagtgac gaccgttttc gtggatcggg tgatcggggt
      241 gatttctctg gtggggcttg caggggtggt ctgcctggcg aactggcgag acccggcttt
      301 ttcgaaatgg gggcagatta tcgggctgac ggttctcgtg ttgctggttg gcggggccgt
      361 ttatttctcc ggccgtctgc ggcgactgct ccggatcgac cggtttctgc tctatctgcc
      421 tctcgggtcg cacattcatc ggatcgacca ggcggcgctt gagtttcgga accgccccgg
      481 gcgtgtggca ggggggctgg tgctcaccgt tgttctgcag gtgatcgcca tcatctctca
      541 ttttctgatt gggtgggggt tgaacatggt cggcgaagac ccctgggggg cgttgccggt
      601 ctatctggcg tttgtgccga tctgttttct ggccggtgcc ttgcccatcg gggcgatgga
      661 actgacctat gtcgagcttt tcgcgcgcag cgcgggtctg ggaacacccg agacagcgac
      721 cttcctttcc ctcctcagcc ggctgatcca gttgctttgg gcgatgccgg gtgtactcgt
      781 cgtcttgaag gccggcaggc cccagccgat aacagattcc gaaccggcgg atgcagccgt
      841 cgcatccgag gggcggcctc actagcagat gcgctctggc catcttgcac gtcatggtcg
      901 gggcggcgaa ggatcccgac tttcacacgg ggtttccaaa cgtccgcgaa gagacgaatt
      961 cggatcagca ggactgatcg gggtgccgcg tgttggtcgc gaccgataag gtggtcttca
     1021 atctttgacg ctgtttccgc cggatgctct tgaattccgc cttggcggct tgaacaatcc
     1081 agcagcccga accgtcggct ggtccgggcc ggggtacgca gctcgtggag ttgcggcgga
     1141 gaggatggaa gcagttatcg gccgggattc gttagcaggg ctgtttccag tcctaacgcc
     1201 tgcgttgaca ggaaccttgg tgtccacgat tatacggtgc agacacatct tggactgccg
     1261 gcagcccagg atgtattccc attgtgggag ctgcctcgga gcgacactcc tcgataatgt
     1321 taagtatctg tggagtagga atttatgaaa cgccattccc gatcctgcct gggcttggtt
     1381 ctgatcgcgg gccttttcat gtgcgtgccg tttcttgtcg ggcagggctg cctgccggac
     1441 agcgggcggc cgacgcccgg tgattccctg ccagtggtta acgtgatcgc gccgtcggtg
     1501 aaccggacgg ccgtggcggg tgacacgatc acggtgcttt acgatgtgtt gaacccacca
     1561 gtggggtccg ctggggccat ccaggtgtgg gggttttacg atcgtgacgg cgtttacgac
     1621 ggagacgaac agacttttgc atcatcgctg gcgagcgggc agggcaagtt cacttccttt
     1681 aacacgagcg gtatcagccc cgggccgctc tacatcggca ttgccgccga gaatgcggcg
     1741 ggcaagaccg tcgattatgc gccggctcgc attacgctga gcgccactgc cgcggtcacg
     1801 ttttcggcgc cggatgggga cctgacggtg gggccggggg cgaccattcc gatcgtgttc
     1861 agcgcgggca gcggcgtcac caacttcacc tggagcctgt tctatgatac ggacggcgcc
     1921 ttcaacggtg atgagttcgt gatcgagacc ggtgcgaaca ccacttcttc cgttgtcagc
     1981 agggagtggg ccattccgct cgacacgccg ccggggacct actacatcgg
//
LOCUS       DAOTPW010000430         2011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_43597_length_2011_cov_3.644172, whole genome shotgun sequence.
ACCESSION   DAOTPW010000430 DAOTPW010000000
VERSION     DAOTPW010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43597_length_2011_cov_3.644172"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="P5316_21815"
     CDS             <1..758
                     /locus_tag="P5316_21815"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR87652.1"
                     /translation="FENPAPHAGHRIVLYGPGGVGKTSLADLAPGPVASFDLDDSHAV
                     LHPKHTKRIGGVESWDDLRAVLNGEGWDKVRTIVVDSATRAEEFAVAWAIANVPHEKG
                     NRISRIEDYGFGKGYQHVYETFLTLLGDLDRHVRTGRNVILICHDCTTSVPNPQGEDW
                     QRYEPRLQSPSSGKASIRLRVREWADHVFFLGYDVDVKDGKGHGCGTRTLWPVEMPHC
                     MAKSRRLTDPIPVYPNDAAVWNALFSQPEAKEG"
     gene            764..1525
                     /locus_tag="P5316_21820"
     CDS             764..1525
                     /locus_tag="P5316_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87653.1"
                     /translation="MNANREGRFRACVMDVGVNETGPNKLCTLVLRLGLTEEYADGEW
                     RNIEAEELDVVAYCYIEKRDGSLNDFQIDMLKDAFGWPGLDPFWFEEQRELPQVQITL
                     QWDEYQGKKRIRVQFINAYDSEPTAGITHADADQRRAMIARLGHKLRAHSGGRTVPSP
                     KPTGSPKPPAPAPQASRQPEKPAPPQPKAKSASTMDDVWTLFAKWAETKGIAEAELNK
                     TWFAAIKEVCGHEEAERVTPEQWPEVAGKLPDCPF"
     gene            1547..>2011
                     /locus_tag="P5316_21825"
     CDS             1547..>2011
                     /locus_tag="P5316_21825"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRR87654.1"
                     /translation="MQCSLREYQRQAVDRLKAVLRRRPILVAPTGSGKTVMAVALVEE
                     MGLPTLWLAHRKELIDQAAAHLEGLGLWPGRIMSGYAPAPLARVQVASVQTLIRRALP
                     AADLVVIDECHHARAGSYEQILEHYPQAQVVGLTATPFRLDGRGLGDVFGEIV"
BASE COUNT          381 a          665 c          645 g          320 t
ORIGIN      
        1 gcttcgagaa ccctgcgccc catgccgggc accggatcgt gctgtacggt ccagggggcg
       61 tcggcaagac gagcctggcc gatctcgcgc cgggccctgt cgctagtttc gatctggacg
      121 actcgcacgc cgtccttcac ccgaaacaca ccaagcgcat cggcggggtc gagtcgtggg
      181 acgacctgcg cgccgtgctc aatggcgagg gctgggacaa ggtgcgcacc atcgtagtcg
      241 acagcgccac gcgcgcggag gagttcgccg tcgcgtgggc cattgccaac gtcccccacg
      301 agaaaggcaa ccgcatcagc cggatcgagg actacggctt cgggaagggc taccagcatg
      361 tctacgagac tttcctgacg ctcttgggcg acctcgaccg ccatgtccgc accgggcgca
      421 acgtgatcct gatctgccat gactgcacga ccagtgtgcc caatccccag ggcgaggact
      481 ggcagcggta cgagccgcgt ctgcagtcgc cgtcgagcgg caaggcctcc atccggctgc
      541 gtgtccggga atgggccgac catgtcttct tcctggggta cgacgtggac gtgaaggacg
      601 gcaaggggca cggctgcggc acgcgcacgt tgtggcccgt cgagatgccg cactgcatgg
      661 cgaagagccg tcgcctcacc gacccgatcc ccgtgtatcc gaacgacgcc gccgtctgga
      721 atgcgttgtt cagtcagcca gaagcgaagg agggatagat cctatgaacg cgaatcgtga
      781 aggacgtttc cgcgcctgtg tcatggacgt aggggtcaac gagaccggtc ccaacaagct
      841 ctgcaccctg gttctgcgcc tgggtctcac cgaagaatac gcggatggcg agtggcgcaa
      901 catcgaggcc gaagaactcg atgtcgtggc ctactgctac atcgagaagc gcgacggcag
      961 tctcaacgac ttccagatcg acatgctgaa ggacgccttc ggctggcccg gcctggaccc
     1021 gttctggttc gaggaacaac gggagcttcc ccaggtccag atcacgctgc agtgggacga
     1081 gtaccagggc aagaagcgca tccgagtcca gttcatcaac gcctacgact cggagccgac
     1141 cgccggcatc acccatgccg acgccgatca gcgccgcgcc atgatcgccc gactgggcca
     1201 caagctccgc gcccattccg gcggcaggac cgtaccctcg cccaagccca cgggatctcc
     1261 caagccgccc gcgcctgctc cacaggcgtc gcggcagcca gagaagccag cgccgccgca
     1321 accgaaggcc aagtccgcat cgacgatgga tgacgtctgg acgctctttg ccaaatgggc
     1381 ggaaacgaaa ggcattgccg aggcggaact caacaagacc tggtttgccg ccatcaagga
     1441 agtgtgcggc cacgaggagg ccgaacgcgt cacgccggag caatggcccg aggtcgccgg
     1501 caagctccca gactgtccat tctaatcggc gctggcggat tcccccatgc aatgttcact
     1561 ccgggagtat cagcggcaag cggtcgaccg ccttaaggcg gtcttgcgtc ggcggcccat
     1621 cctcgtcgct ccaaccgggt cgggaaagac cgtaatggcg gtcgccctgg tggaagagat
     1681 gggcctgccg acgctgtggc tggcgcaccg caaggaactc attgaccaag cggcggcgca
     1741 cctcgaaggt ctcggcctct ggccgggccg gatcatgtcc ggctacgccc cggctcctct
     1801 ggccagggtt caggttgcca gcgttcagac gctgatccga cgcgctttgc ctgccgctga
     1861 tctggtggtc atcgatgaat gccatcacgc ccgggccgga agctacgagc agatccttga
     1921 acattatcca caggcgcagg tcgtcggact gacggcgacg ccgttccggc tggatggccg
     1981 cggcctcggc gacgtattcg gcgagatcgt c
//
LOCUS       DAOTPW010000431         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_44076_length_1996_cov_5.464194, whole genome shotgun sequence.
ACCESSION   DAOTPW010000431 DAOTPW010000000
VERSION     DAOTPW010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44076_length_1996_cov_5.464194"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="P5316_21830"
     CDS             <1..308
                     /locus_tag="P5316_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87655.1"
                     /translation="ELLEELEIENGFVQEIEGVESPSAYLGVLGMTGLTAWVGIREIG
                     KPDPSRPEMKPRCDGLVALADAVVALCSNIAMEKKKRIVMNPKWFDPSDPANPESEV"
     gene            complement(446..>1996)
                     /locus_tag="P5316_21835"
     CDS             complement(446..>1996)
                     /locus_tag="P5316_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87656.1"
                     /translation="HIPKGWLRGRSVPTDGTQMLATDMNGVSCDFCHRLVDPIYTPGV
                     SPIGDQAILAGMLSVPNTFANGMFVIDPTGARRGPFVDATSGHPILVSPFHREAALCG
                     TCHDVSNPAFEKDLHGNYPPNAFDTPAASFYSHTLMPIERTYSEWFFSDYNTPAGVYA
                     PQFGGNKQYVATCQDCHLRDVTGKGCNFGNPPTRTDLPLHDMTGGSAWLSGLLPTMFP
                     GQAGAAAMQSGQMRSRYMLQNAASMAAAQDGTRLKVTVTNETGHKLPTGYPEGRRMWI
                     NVKFYDQDSNLLAESAAYDAESGTLSHDAAARIYEAKPGLDVDVASLAGEDPGPSFHF
                     VLNNKIYKDNRIPPRGFSNALFASFGGAPVGHEYDDGQYWDDAWYAVPFGAVSATVTL
                     YYQSTSKEYVEFLLAENTTNNAGQVMHDLWNNNGKAPPELMKTVTVAVSIPDVRADLD
                     HDGDVDEDDMILFEACASGPGVPHSGTAECQRVDFDTDSDVDQSDFSIEQACFSGANI
                     LADPACSS"
BASE COUNT          361 a          600 c          639 g          396 t
ORIGIN      
        1 ttgaactgct ggaggagttg gagatcgaga acggctttgt tcaggagatc gagggcgtcg
       61 agtccccgag cgcctatctg ggcgtcctcg gcatgaccgg tctgaccgcc tgggtcggga
      121 tccgcgagat cggcaagccc gatcccagcc ggcctgagat gaagccccgc tgtgacggct
      181 tggttgccct ggccgacgcg gttgtcgccc tctgctcgaa catcgccatg gaaaaaaaga
      241 agcggatcgt catgaacccg aagtggttcg atccgagcga cccggccaac cccgagtcgg
      301 aagtctgatc acggtctgca aaaaaaccgc aagacgcggc ggccggcacg aaaacgccgg
      361 ccgcttctgt ttggagagtc tatcagcgat cagtgttcag caatcgacaa gagagcaact
      421 gggtcgccac aatgccatga cgatgtcagc tcgagcaggc gggatcggcc aagatgtttg
      481 ctccgctaaa gcacgcctgc tcgatgctaa aatcggactg gtcgacgtcg ctgtccgtgt
      541 cgaagtccac cctttggcat tcggccgtgc cactgtgggg aacacccggg cctgaggcac
      601 aggcctcgaa caggatcatg tcgtcctcgt ccacgtcgcc gtcatgatcg aggtccgctc
      661 ggacatccgg aatggacacc gccacggtga ccgtcttcat cagttccggc ggggccttgc
      721 cgttgttgtt ccacagatcg tgcatcacct ggcccgcgtt gttggtggtg ttctcagcta
      781 gcaggaattc cacgtattcc ttgctggtgc tctggtagta gagcgtcaca gtggccgaga
      841 cggcgccaaa cggcaccgcg taccatgcgt cgtcccagta ctgcccgtca tcgtactcat
      901 gacccaccgg ggccccgccg aagctggcga acagggcgtt gctgaagcct cgcggcggga
      961 tgcggttatc cttgtagatc ttgttgttca ggacgaaatg gaacgacggg ccgggatctt
     1021 cgcccgccag ggaagccacg tcgacgtcca gacccggctt ggcctcgtag attctggcgg
     1081 cggcatcatg actcagcgta cctgattcgg cgtcgtaggc cgccgactca gccagcaggt
     1141 tgctgtcctg gtcgtagaac ttgacattga tccacatgcg tcgcccttcg ggatagcctg
     1201 ttgggagttt atggccggtt tcgttggtca ccgtcacctt caggcgcgtg ccgtcctgtg
     1261 ctgcggccat gcttgccgcg ttctgcaaca tgtaacgaga tctcatctgc ccgctctgca
     1321 tcgcggcggc gcctgcctgc ccggggaaca tcgtcggcag aaggccgctc aaccacgcac
     1381 tgccgccggt catgtcgtgc aggggcagat ccgtccgcgt aggcgggttg ccgaagttgc
     1441 agccctttcc ggtcacgtcg cgaaggtggc agtcctgaca ggtggcaaca tactgcttgt
     1501 tcccgccgaa ctgcggggca taaacacccg cgggcgtgtt gtagtcgctg aaaaaccact
     1561 cgctgtaggt ccgctcgatg ggcatcaggg tgtgcgagta gaaactcgcc gcgggcgtat
     1621 cgaacgcgtt cggcgggtag ttgccgtgga ggtccttttc aaaggcggga ttgctcacgt
     1681 cgtgacatgt cccgcagagt gccgcctcgc ggtggaacgg ggagaccaga atcggatgcc
     1741 cgctggtggc gtcgacgaac ggtcctcggc gggcccccgt ggggtcgatg acgaacatgc
     1801 cgttggcgaa ggtgtttgga accgacaaca tgccggcaag gatggcctgg tcccctatcg
     1861 ggctgacacc cggcgtatag atgggatcga cgagtctgtg gcagaagtcg cacgagacgc
     1921 cgttcatatc cgtcgccagc atttgcgtcc cgtcggtcgg gaccgatcgg cctcgcagcc
     1981 agcccttggg aatatg
//
LOCUS       DAOTPW010000432         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_44358_length_1987_cov_5.596791, whole genome shotgun sequence.
ACCESSION   DAOTPW010000432 DAOTPW010000000
VERSION     DAOTPW010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44358_length_1987_cov_5.596791"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="P5316_21840"
     CDS             complement(<1..746)
                     /locus_tag="P5316_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRR87657.1"
                     /translation="MKRPAHLRIHLQQPRIIRAFTLIEILVVVAIIALLIAILLPSLS
                     KAREYTRNTICKSNMRQVMLGHMFYQADHKVLPASQSSFYLRGIWPLPDPETNLGPDG
                     RLKGMTWEGARGVSGYYTKPYYTDPKFLRQVPQIGTIFRYTKNVEIYLCAADKPGTAD
                     ETPQGGGGNGRLSYSMNAYIGCKTLEELGSFVYVEAVSNAPLPGGVRYRSFKFGERVV
                     FAPSQVMYLFEEHPYDNINNGYPENNFNTQ"
     gene            complement(977..>1987)
                     /locus_tag="P5316_21845"
     CDS             complement(977..>1987)
                     /locus_tag="P5316_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRR87658.1"
                     /translation="GPGRREPGGEPGAVAAGGRTTTDGIYVMTADQGDVLGQGDTAFA
                     GGINWTTGGSNSPWKITVAPDDSVYIADWSDLNSGVWRCNANPQSNFDEILTNVGRTS
                     TGLVAGVHGSVASVYIEGTGANTVLYTLDEDFNAGSGMGSILRYDIGTQTGYSGTPVE
                     QTQDIPNHIQNSNMDFVRDSDGSWWICQYRYTDSDAVPTLLHWADGASSPSWKSGPST
                     VALDRGYGILAIDNLNDLIAVGTNNSKIYILDISDPSNVALVDTIAHSGATIRDLDFD
                     AAGNLYVVSSSSETLRIYSPGGDWMAITGSDGSFVLIPEPAAVVLLALGGLACLRRRR
                     IA"
BASE COUNT          387 a          610 c          583 g          407 t
ORIGIN      
        1 tactgggtat taaaattgtt ttcggggtat ccgttgttga tgttatcgta cggatgctcc
       61 tcgaacaggt acataacctg cgaaggggca aagacgacgc gctctccgaa cttgaacgac
      121 cggtagcgaa ccccccctgg caaaggcgcg ttgctgaccg cctcgacata aacgaagctt
      181 cccagctcct cgagcgtctt gcagccgatg taggcgttca tcgagtagct gagtcgtccg
      241 ttgcctccgc cgccctgggg agtctcatcg gcggttccgg gtttgtcggc ggcacacaag
      301 tagatctcga cgttctttgt atagcgaaat atggtgccga tctggggcac ctgccgaagg
      361 aatttcgggt ccgtgtagta aggcttggtg taataaccgc tgacgcccct tgctccctcc
      421 catgtcatgc ctttaagacg cccgtccggt ccgaggtttg tttccgggtc cggcagcggc
      481 caaatccctc gcagatagaa gctgctctga ctcgccggca agaccttgtg gtccgcctgg
      541 tagaacatgt gccccaacat gacctggcgc atgttggact tgcagatggt gtttcgcgtg
      601 tattcgcgag ccttgctgag agaaggcaac aatatggcaa tcagcagggc gatgatcgcc
      661 acgacgacca ggatttcgat cagagtgaag gcccggatta tgcggggttg ttgaagatgg
      721 atccgcaggt gagcaggtct tttcattgcc gtggctcaca gtgacagggg cgttcggccg
      781 cgcggcgcgc ttggcgcggt acccacactt gacaattccc attataacca gccgcccggg
      841 aaagacaata ggcccatccc gggcgtccgg gaaactactg aacagacgct ccaacccggg
      901 gggtcctcct gcggggcccc cgccgggttg gcgtcttaat ccttgggcat gccaagcatt
      961 gcccgtctca gttgcgtcag gcaatccgtc gccgacgcag gcaggccagc ccgcccaggg
     1021 ccagcagaac gaccgccgca ggctcgggaa tcaggacaaa agagccgtca gagccggtaa
     1081 tggccatcca gtcgccgccg ggcgagtaaa tccgcaacgt ttcgctgctg ctgctcacga
     1141 cgtacagatt gccggccgcg tcaaagtcca aatcgcgaat cgtggcaccc gagtgcgcga
     1201 tcgtatcaac cagcgcgacg ttggacggat cgctgatgtc caagatgtag atcttgctgt
     1261 tgttcgtgcc gacggcgatc aggtcgttga gattgtcgat cgccaatatc ccataacccc
     1321 gatcgagggc gaccgtgctg ggccccgact tccaagacgg agagctcgcc ccgtccgccc
     1381 agtgtaagag cgtaggcacc gcgtcactgt cggtgtagcg gtactggcag atccaccaac
     1441 tcccatccga gtcacggaca aaatccatgt tggaattctg gatgtggttg gggatgtcct
     1501 gggtctgctc aacgggtgtc ccgctatagc cggtttgcgt gccgatgtca taccgcagga
     1561 tgctgcccat gccgctgccg gcattgaagt cctcatccag cgtgtacagc actgtatttg
     1621 ctcccgttcc ctcaatgtac accgacgcta cgctaccgtg cacgccggca accaagccgg
     1681 ttgacgtccg gccgacgttg gtcaggatct catcgaaatt cgactgggga ttggcgttgc
     1741 atcgccacac gccggagttc agatccgacc agtccgcaat atacacactg tcatccgggg
     1801 ccacggtgat cttccacggg ctgttcgatc cgccggtagt ccaattgatg ccgcccgcaa
     1861 aagcggtgtc accctggccc agaacgtcgc cctgatcagc ggtcatcacg tagatgccgt
     1921 cggtcgtggt acgcccgccg gcagccacgg ccccaggctc gcctccaggc tctcgcctcc
     1981 caggacc
//
LOCUS       DAOTPW010000433         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_44855_length_1971_cov_6.273486, whole genome shotgun sequence.
ACCESSION   DAOTPW010000433 DAOTPW010000000
VERSION     DAOTPW010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44855_length_1971_cov_6.273486"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /gene="rsgA"
                     /locus_tag="P5316_21850"
     CDS             <1..642
                     /gene="rsgA"
                     /locus_tag="P5316_21850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRR87659.1"
                     /translation="IREPMIKPHLIDRYLVAAHAGNLPAAICVNKVDLDPDPEDETAE
                     VLTRYRRLGYAAVATSAVEGTGLDELRSLLTGKVTLLAGQSGVGKSSLINALEPGLNL
                     KTAPVSLASEKGRHTTTTAVRLKLSFGGAVVDTPGIRALDVAMVPINELEMHFVEFVD
                     RIANCKFPNCVHIHEEGCEIKAAVKSGEIDESRYASYVELFYELSDVKRAGYE"
     gene            complement(842..>1971)
                     /locus_tag="P5316_21855"
     CDS             complement(842..>1971)
                     /locus_tag="P5316_21855"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRR87660.1"
                     /translation="QHAVLDEYIPFIILLFCLYTISGGIQLRGDLRARPATNAAFLGL
                     GAVLASFIGTTGASMLLIRPLLQINQERKHVRHTVIFFIFLVSNIGGSLLPTGDPPLF
                     LGYLFGVPFLWTLNLWKAWLFAVCVLLVVYWIWDTLAYRRETVEDRMLDATQIEPLRI
                     RGSLNFLWLLCVVLAVAVMVPGKELPGTSFAVPDFLREAVMLAFVGLAWMTTSPQIRK
                     ENNFDFCAITEVAVLFIGIFITMQVPIEILNAKGPELGLTKPWQFFWATGTLSSFLDN
                     APTYVVYFKTAGTLAPEGMAMMGDVDTFLKTIPIPLLVGISLGAVFMGANTYIGNGPN
                     FMVKSIAEQSGIKMPSFFGYMLYSIVILIPLFLLITVIFLM"
BASE COUNT          459 a          574 c          587 g          351 t
ORIGIN      
        1 attcgcgagc cgatgatcaa accgcacctg atcgaccgct atctggtggc ggcgcacgcg
       61 ggcaacctcc ctgcggccat ctgtgtaaac aaggtagacc tcgaccccga tcccgaagac
      121 gagactgcgg aagtgctgac ccgttatcga aggttgggtt atgccgccgt agccacaagc
      181 gccgtcgaag gtacgggcct ggacgagttg cgttcgctat tgacgggaaa ggtgaccctt
      241 ctggcgggtc agagcggcgt cggcaagagt tcactgatca atgcgctcga accgggtctc
      301 aacctcaaga cagctcccgt ttcgctcgcc agcgagaagg gacgccacac gaccacaacc
      361 gcagtgcggc tcaagctcag cttcggcggc gcggtggttg acacgccggg tatcagggcc
      421 ttggacgtgg ccatggtgcc gatcaacgaa ctcgaaatgc acttcgtcga attcgtcgat
      481 cgtatcgcca actgcaagtt ccccaactgc gtccacattc acgaggaggg gtgcgagatc
      541 aaggccgctg tgaaaagcgg cgagatcgac gagtcccgct atgccagtta tgtcgagctg
      601 ttctacgaac tcagcgatgt taagcgggcc ggatacgaat aggtctccgc cggataccat
      661 cgaaaagcca cgtcacgcat cgcgccttgc tcggctcggt cttgctcatc ggggacgcgg
      721 caaatggacg attgtcgatc acgaacggcg cagaccccaa tcctgtttgc ccaggctatc
      781 gtcatcgcgc aaaaccggtt cgacccgtcc ttgccggccg attcccgatc gctgaaccct
      841 ctcacatgag gaagatcacc gtgatcagca ggaaaagcgg aatgagaatc acgatactgt
      901 agagcatgta gccgaagaaa cttggcattt tgatgcccga ctgctcggca atgctcttga
      961 ccatgaagtt cgggccgttg ccgatgtagg tgttggcgcc catgaagacg gcgccgagcg
     1021 agatgccgac cagcagcgga atcgggatgg tcttgaggaa cgtatcgaca tcgcccatca
     1081 tggccatccc ttcgggcgcc agggttccag cggtcttgaa gtagaccaca taggtcggcg
     1141 cattgtcgag gaagctggac agagtgcccg tcgcccagaa gaactgccac ggcttggtca
     1201 ggccgagctc gggccccttg gcgttgagga tttcgattgg aacctgcatc gtgatgaaaa
     1261 tgccgatgaa cagcacggcc acctcggtga tggcgcagaa atcgaagttg ttttccttgc
     1321 ggatctgcgg cgacgtcgtc atccaagcca ggcccacgaa cgcaagcatc accgcttcgc
     1381 ggagaaaatc gggcacggcg aaacttgtgc ccggcaattc cttgccgggg accatgacgg
     1441 ccaccgccag cacaacgcaa agaagccaca ggaaattcag cgaaccacgg attcgcaacg
     1501 gttcgatctg cgtggcatcc agcatgcgat cttcaacggt ctcgcggcga tacgccagcg
     1561 tatcccagat ccaatatacg accagcaata cgcagacggc gaagagccag gctttccaga
     1621 ggttcagtgt ccaaaggaag ggcacaccaa acaggtaacc gaggaacaga ggcggatcgc
     1681 ccgtcggaag cagactgccg ccaatgttgc tgaccaggaa gatgaagaag atcaccgtgt
     1741 ggcggacgtg cttacgctcc tgattgatct gcaacagggg ccggatgagc aacatcgatg
     1801 cacccgtggt tccgatgaag ctcgcgagga ccgcgcccaa ccccagaaag gctgcattgg
     1861 ttgccggtct ggcccgcagg tcgccgcgga gctggatgcc gccactgatc gtatacaggc
     1921 agaacagcag gatgatgaat ggaatgtact catccagaac agcatgctgc a
//
LOCUS       DAOTPW010000434         1931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_46136_length_1931_cov_4.369403, whole genome shotgun sequence.
ACCESSION   DAOTPW010000434 DAOTPW010000000
VERSION     DAOTPW010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46136_length_1931_cov_4.369403"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /gene="pyk"
                     /locus_tag="P5316_21860"
     CDS             complement(<1..1259)
                     /gene="pyk"
                     /locus_tag="P5316_21860"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRR87661.1"
                     /translation="MIRTRLVATVGPACSDAQTLGAMIDAGVNVFRLNFSHGTLDTHQ
                     QMLQHIRSEAGRRQAMVAVMGDLCGPKIRLNRIDGGKCELIKGATVVFRRGDALGNAQ
                     TLTSSYPALLDDVQVGHRLLIDDGNVQLRARSKTADELVCEVEVPGVISDRKGINLPD
                     SKVSSPALTGKDREDLEWAISNDLDFVALSFVRRPEDLIELREIVLRHNSPIHLVSKI
                     EKPEAVDSLQSIIDQSDVVLVARGDLGVEMDVSRVPLIQKKICLLCRRSRKPVIVATQ
                     MLQSMVESPVPTRAEVSDVANAILDHADAVMLSAETSVGKYPLPAVRMIRRIAEQTET
                     FHDQFRHDLGGDSLIRPCLDMAVADGAVVVAERLQTRLIAVWTRKGDAARLLSKHRVH
                     QPILAVTDDERVCRKMAMLYGVVAVCHP"
     gene            complement(1520..>1931)
                     /locus_tag="P5316_21865"
     CDS             complement(1520..>1931)
                     /locus_tag="P5316_21865"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRR87662.1"
                     /translation="GLALANAGLGAAHGIAAPLGGRFAAPHGAVCAALLPHVITVNVA
                     AIRRAAPQSESLRRYDEVGRMLTGSASAVAADAIRWTSEVNRDLGIPRLGSYGIRPID
                     IPSLAEGARSASSMKGNPVVLTSEEVCAIVTAAL"
BASE COUNT          375 a          588 c          616 g          352 t
ORIGIN      
        1 ggatgacata ccgcgacgac accgtagagc atggccatct tgcgacagac tcgttcgtca
       61 tccgtgaccg caaggatggg ctggtggacg cgatgtttgc tgagcaatcg cgccgcgtcc
      121 cccttccgcg tccaaacggc gatcaatcgg gtctgaagcc gctcggccac cacgactgcc
      181 ccgtcagcca ccgccatgtc cagacaaggc ctgatcagcg aatcgccgcc aagatcgtgc
      241 cggaattgat catgaaaggt ctcggtctgt tcggcgatgc ggcgaatcat ccggaccgcc
      301 ggaaggggat acttgccgac cgaggtctca gccgaaagca tgaccgcatc ggcatggtcg
      361 agaatggcat tggcgacatc gctgacctcg gcccgggtgg gaaccggcga ttcgaccatt
      421 gactgcagca tttgcgtggc gacgatcacc ggcttgcgag agcgtcggca caacaggcaa
      481 atcttcttct ggatcagcgg cacacgcgag acgtccatct caacgccgag atcgccgcga
      541 gcgaccaaga caacgtcgga ctggtcgatg atcgactgaa gcgaatcgac cgcttcgggt
      601 ttttcgattt tggagacaag atgaatgggg ctgttgtgcc gcaggacgat ctctctgagc
      661 tcgataaggt cttccggccg tctgacgaac gacaaggcca cgaaatcaag gtcgttggaa
      721 atggcccatt ccagatcttc gcgatccttg ccggtcaggg caggtgatga cacctttgaa
      781 tccggcaggt tgataccctt gcgatcgctg attactccgg gaacctcgac ctcgcagacc
      841 aattcgtcgg cggtcttgga gcgggcccgc aactgcacat tgccgtcatc gatcaagagc
      901 ctgtgcccga cctggacgtc gtcaagaagg gcagggtagg aactggttag agtctgggcg
      961 ttccccaggg cgtctccgcg ccgaaaaacc accgtggccc ctttgataag ctcgcacttg
     1021 cccccgtcga tgcggttgag gcggattttg gggccgcaca ggtcgcccat cacagcaacc
     1081 atggcctgac gccgtccggc ctcgcttcgg atgtgctgaa gcatctgctg gtgcgtgtcc
     1141 agcgtgccgt gcgagaagtt gagccgaaag acgttgacgc cggcgtcgat catggcaccc
     1201 agggtctggg cgtccgagca ggcaggtccg acggtggcga cgagtctggt cctgatcatg
     1261 gacgatagtg tatgcggaca aaccggcttt gtacaatagc agctattgga gcatatgggt
     1321 tgctgattgc ctgcgggcct gcctctcgcc gggtctgata ccaggaccgt catgccgggg
     1381 caacgccgtc gtccagacgg ccgacgggcg atcgaaacgc cctgtggacc ggctcagact
     1441 atccggcacg aacgcccccg acgttgtggc tcggagaaat gacgaacttt tctgaaacag
     1501 cgccttcgag ctacggttat cagagcgcgg cggtgacgat tgcgcacact tcctcggacg
     1561 tgagtacgac cggattgccc ttcatgctgc tggcgctgcg ggcgccctct gccagagagg
     1621 gaatgtcgat cggccggatc ccatacgagc cgagcctggg tatcccgaga tcgcggttga
     1681 cctcgctggt ccagcgaatg gcatcggcgg ccacggccga ggcgctgccg gtgagcatgc
     1741 ggcccacctc gtcgtagcgg cggagactct cgctctgagg cgcggcccgg cgaatggccg
     1801 cgacattgac ggtgatgacg tgcggcagca aggcagcaca tacggctccg tgcggagcgg
     1861 caaacctgcc gccaagcggg gcggcgattc cgtgcgcagc ccccaatccg gcgttggcca
     1921 gcgccaagcc g
//
LOCUS       DAOTPW010000435         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_46199_length_1929_cov_5.304162, whole genome shotgun sequence.
ACCESSION   DAOTPW010000435 DAOTPW010000000
VERSION     DAOTPW010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46199_length_1929_cov_5.304162"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="P5316_21870"
     CDS             <1..493
                     /locus_tag="P5316_21870"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRR87663.1"
                     /translation="TFLEQHKFVSHEIGVGAIDTTVHYVTDEHGQTTREVASYTLSRT
                     FFVTTDDVDRVMQTAGLVTQLIQEGVLVISQSPAYYYSDPSPLKVELMGLASKDARGR
                     AEEIARSAGCKLGQVRAAHMGVLQITRPYSTDVSAEGIYDISTIEKDAQAVVTVTFQI
                     VDE"
     gene            622..>1929
                     /locus_tag="P5316_21875"
     CDS             622..>1929
                     /locus_tag="P5316_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87664.1"
                     /translation="MARDRIIFRVALLTLLPTTLVGAIVLNWKPGMPIAALVAHLCAG
                     LSFLLMLPVYAGGGRCAGYAPSASCLRLMHVLKLGAVTAGLSAVLMMLYAVVVEILFR
                     VLMWEHGATPRVPIWTTGFADLALVGGAIALAWHCSVNREMLTALLWLAVFAAVWAAL
                     QIPAFSTDAEGRVIMSRWLNILALLMALVAGAFVAATGWAHRRRRRRAWPAHLDLLAE
                     PCTDRPAFRYSMGILASIILLLGCTQLVLPLTGPAGLMSGLAVLVLAHRQWNENLADV
                     GLALITLGVVSLVVTCFPALPFWWTASAWAEVFNRVLFALAIMAGVWFWFAAFWRQQL
                     EDGHAWTTTGYLIQTARQVGFFVAAVGVLISFYLAFWPVLPYGSADSGTWRWGWALGA
                     NGLLILVLVLAVRRTMKSTIGWLLALSLASTVSRSWTSSSAEPQ"
BASE COUNT          286 a          537 c          647 g          459 t
ORIGIN      
        1 gacgttcctt gagcaacaca agtttgtttc ccatgagatc ggggtggggg ccattgacac
       61 gaccgtgcac tacgtaaccg acgagcacgg gcagacgacc cgggaagtgg cctcatacac
      121 tctgagccgg acgttttttg tgacaaccga cgacgtggac cgagtcatgc agacggcagg
      181 actggtgacc cagttgatac aagagggtgt gctggtcatt tctcagagtc cggcgtacta
      241 ctacagcgat ccgtcgcctc ttaaggtgga gctgatgggt ttggcttcca aggacgcccg
      301 cggccgggcc gaggagatcg cgcgcagtgc cggctgcaag ctggggcagg ttcgagctgc
      361 gcatatggga gtgcttcaga tcacgcgtcc ctactccacg gacgtcagtg cggaaggcat
      421 ctatgatatt tcgaccatcg aaaaggacgc ccaggcggtt gtcacggtca ccttccagat
      481 tgtcgatgag tagccgggct tgcggctccg attgacgggc tatggtaatc cgctacgctg
      541 tcagaggtga gccgtgggca aggcggcgcc ggatgacgca agcctgggtg catcgggctc
      601 gtgcctgaac ggagtacgtc ggtggcgagg gatcgcatca ttttccgggt ggccctgctg
      661 accctgctgc ctaccacgtt ggtcggtgcg attgttctga attggaaacc cggcatgcct
      721 atcgcggccc tggttgcgca tctttgcgcc ggtttgtctt ttctgttgat gctgccggtt
      781 tacgcggggg gcgggcggtg tgccgggtat gcccccagtg cttcgtgcct caggctgatg
      841 catgtgctga agctgggcgc tgttacggcg ggtttgtccg ccgtgctcat gatgctttat
      901 gcggttgttg tcgagattct ttttcgggtg ctcatgtggg agcatggggc gacgcctcga
      961 gtgcccatct ggacaacggg gtttgccgac ttggcgttgg ttggcggtgc gatcgcattg
     1021 gcctggcatt gcagtgtcaa ccgtgaaatg ctgaccgcgt tgctgtggct ggcggttttt
     1081 gcggccgtgt gggctgctct tcaaataccg gccttcagta ccgacgctga aggtcgtgtc
     1141 atcatgagcc gttggctcaa catactggcc ttgctgatgg ccttggttgc cggggcgttt
     1201 gtcgccgcaa cagggtgggc acaccgtcgc cgaagacggc gtgcgtggcc ggcccatctc
     1261 gacctgctcg cggaaccctg cactgacagg ccggcgtttc gttacagcat gggtatcctg
     1321 gcatctatca ttctgctcct gggatgcact caactcgtgc tgccgctgac cggtccggcc
     1381 ggcctcatga gcggattggc cgtcctggtg cttgcgcacc gccagtggaa cgagaacctg
     1441 gcggatgtcg ggctcgcgct catcacgctc ggcgtggtct ctctcgtggt gacgtgcttt
     1501 ccggcgttgc ctttctggtg gaccgcctcg gcatgggcgg aggtcttcaa cagggttctc
     1561 tttgcgctgg cgatcatggc cggcgtgtgg ttctggttcg ccgccttctg gcgacagcag
     1621 cttgaggatg gtcatgcttg gacgaccacc ggttatctga ttcagaccgc ccggcaggtg
     1681 ggctttttcg tggcagccgt gggcgtcctg atctcattct atctggcatt ctggccggtt
     1741 ctcccctatg gcagcgctga tagcggcact tggcgatggg gctgggccct gggagccaac
     1801 ggtctgctga tactggtcct ggtgcttgcg gttcggcgga cgatgaagtc cacgatcggc
     1861 tggctgttgg cgctttcttt ggcctcgacg gtcagccgat cgtggacttc atcgtccgcc
     1921 gaaccgcaa
//
LOCUS       DAOTPW010000436         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_46719_length_1914_cov_5.666487, whole genome shotgun sequence.
ACCESSION   DAOTPW010000436 DAOTPW010000000
VERSION     DAOTPW010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46719_length_1914_cov_5.666487"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1914
                     /locus_tag="P5316_21880"
     CDS             <1..>1914
                     /locus_tag="P5316_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87665.1"
                     /translation="DDDNDIHPAVGTRDLYIYYRPAVHKLRVPGAIDSYECAGWTSLT
                     GDGSDADGDAARIVFSVSDSPIGAPQGDTDGDGLPDSLEICGSPQINLPAIGADPYRK
                     DVFVEIDWMTEPGTSSPHSHEPWLPALINIWNEFNVMPITNPTVNGVTHPPGIALHID
                     TGLLYANYELDFDGDGTPEIRVGPNGNLDINRDGIVDIGNLGPLAGIGIGGERLAEDP
                     MLGPRLCEPDSDFGAIKAAHFAQSRRGIFRYCVFAHDFDAPCRSGFACNDEFLVTLGC
                     TGYEDSYLVPGPGGVPTPADLRGPSGLRVKGTIKQHAGTFMHELGHTLWLGHGGNDGN
                     NHKPNYLSVMNYTWQFDGISFDADGDHLADALGFDYNRDGRIDNSRFMYSPVALPALD
                     ESNLDESSGIGAAGTALADAITRYGPQLDLDGDHHADILLATARCNQPIDWNRDGDSS
                     DSGVSTASLVLSFSGSSSSTPSDINRWPGEGPSPGQVLNGFNDCLGLQSRGLPVPRVP
                     GASIPADDELTEELWKNMQRNTARVVEVPGEDAIAKLCQQDLETITFEEIKPGTPVNT
                     QYEPLATFVEDAAASYIVEGPNERGGVPTQSPDNSLVVTGSAPLRVTFGQSQRLVPKQ
                     ANSGGSFARSAAD"
BASE COUNT          386 a          584 c          597 g          347 t
ORIGIN      
        1 cggacgacga caacgacatt catcccgccg tcggcacacg cgatctctat atctactacc
       61 gtccggcagt tcataagttg agagtccccg gtgcgatcga cagttatgag tgcgccgggt
      121 ggacatcctt gaccggggac ggcagtgatg cggacgggga cgcggccagg atcgtcttca
      181 gcgtgagcga ttcgccgatc ggcgcgcccc agggtgatac cgatggcgat ggcctgccgg
      241 acagtctgga aatctgcgga tcaccgcaga tcaatctgcc cgccatcggg gcggatccgt
      301 accgcaagga tgtctttgtg gaaatcgact ggatgacgga gcccggcacc tcgtcaccgc
      361 acagccacga gccctggctg ccggcgctga tcaatatctg gaacgaattc aatgtgatgc
      421 cgataacgaa cccgaccgtg aacggagtga ctcatccccc gggcatcgca ctgcatatcg
      481 acacgggctt gctctatgcc aattacgagt tggatttcga tggcgacggc acacccgaga
      541 ttcgggtcgg gcccaacggg aatctggaca tcaaccgtga cggtatcgtg gacatcggga
      601 atctgggccc gttggccggt atcggaatcg gcggcgagcg tctggccgag gatccgatgc
      661 tgggtccgag gctgtgtgag ccggacagtg actttggagc gatcaaggcc gcccatttcg
      721 ctcagtcccg ccggggcatc ttccggtact gtgtcttcgc ccacgacttc gacgcgccct
      781 gccgatccgg ttttgcctgc aacgacgagt tcctcgtgac gctgggttgc acgggctacg
      841 aggactccta ccttgttccg ggcccagggg gagttccgac accggcggac ctgcgggggc
      901 ccagcggttt gagggtgaag gggacgatca aacaacatgc cggcacgttc atgcacgaac
      961 tggggcatac gctctggctg ggacacgggg gcaacgacgg gaacaatcac aagccgaact
     1021 acctgagcgt catgaactat acatggcagt tcgacggcat ctccttcgac gcggacggag
     1081 accatttggc ggacgcgctc ggattcgact acaaccgcga cggtagaatc gacaactcgc
     1141 ggttcatgta ctctcccgtg gccctgccgg ctttggatga gagcaacctc gatgagtctt
     1201 ccggcatcgg ggccgccggc acggccttgg ccgatgcgat caccagatac ggcccgcaac
     1261 tggacctcga tggcgaccac catgccgaca ttcttctggc gaccgcacgg tgcaaccagc
     1321 cgatcgactg gaaccgggac ggcgattctt cggacagcgg agtgagcacc gcgtctctgg
     1381 ttctgtcctt ctcgggcagt tcaagcagca cgccctccga cataaaccga tggccgggag
     1441 agggtcccag cccgggacag gtcctcaacg gtttcaacga ctgcttaggg ctccagagcc
     1501 gcggattacc cgttcctcgc gtgccggggg ccagcatacc ggcggatgac gagcttacgg
     1561 aagagctttg gaaaaacatg caacgcaaca ccgcgcgcgt cgtcgaggtg ccaggtgagg
     1621 atgccatcgc caagctctgt cagcaggatc tcgagaccat cacttttgag gagatcaagc
     1681 cgggcacgcc agtcaatact caatatgaac ctctcgcgac gttcgtcgag gatgctgcgg
     1741 caagctatat cgtcgagggg ccgaacgaac gcggaggcgt tcccacccag tcaccggaca
     1801 actcgctggt ggtgaccggc tctgcgccgt tgcgcgtcac gtttggacag tcccaaaggc
     1861 tagtcccaaa acaagcgaac tccggtggca gttttgcgcg atcggcggcc gatc
//
LOCUS       DAOTPW010000437         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_47542_length_1889_cov_5.424755, whole genome shotgun sequence.
ACCESSION   DAOTPW010000437 DAOTPW010000000
VERSION     DAOTPW010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47542_length_1889_cov_5.424755"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="P5316_21885"
     CDS             complement(<1..616)
                     /locus_tag="P5316_21885"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRR87666.1"
                     /translation="MNRLLTCLVMLVGLFQSANAADRRPNVLLIVGDDHRTEAIGAYG
                     NKIVKTPNLDRLAESGLRFERAYCNSPMCTSSRQSFLIGQYPHATGVTLLNHALTEDT
                     ITLADRLREAGYKTGAFGKMHFNSDKMHGFEVYARPEQFWREHGQRGDKPLPEGMDFL
                     PVWRPFKDPARIWLNSFYRPMDRYDDEMPDTWYVRQAIEFMMKHA"
     gene            803..1027
                     /locus_tag="P5316_21890"
     CDS             803..1027
                     /locus_tag="P5316_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87667.1"
                     /translation="MREIHKKVITDDQMRPVAVQIDYADWVEIERQLAERQLPVADAG
                     PTDLSSFAGTLRWSEDGVAYQRRVREEWDR"
     gene            1024..1386
                     /locus_tag="P5316_21895"
     CDS             1024..1386
                     /locus_tag="P5316_21895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRR87668.1"
                     /translation="MTVLDTNILIYLLEGRLQDELPGSGLCLSVITEIELLSYSRLTE
                     DQKARIQDMVTRLTVIGLTPQVKEAAIQLRRVNRLTIPDAIVAATALSLGAELLTNDD
                     KLMTVPGLACRRMNVAPP"
BASE COUNT          372 a          525 c          593 g          399 t
ORIGIN      
        1 cggcgtgttt catcatgaac tcgatcgcct gacggacgta ccaggtgtcg ggcatctcat
       61 cgtcgtatcg gtccatcggg cggtagaacg aattcagcca gatgcgggcc gggtctttga
      121 acggcctcca gaccggcaga aaatccatgc cttccggcag cggcttgtcg ccccgttggc
      181 cgtgctccct ccaaaattgc tccggccggg cgtatacctc gaagccgtgc atcttgtcgg
      241 aattgaagtg catcttgccg aacgcaccgg tcttgtagcc ggcctcgcgg agccggtccg
      301 ccagggtgat ggtgtcttcg gtcagcgcgt ggttcagaag agtgacgccc gtggcgtggg
      361 gatactggcc gatgagaaac gactgccgcg acgacgtgca catcggcgag ttgcagtatg
      421 cccgctcgaa tctcagcccc gattcggcca agcggtcgag gttgggggtt ttcacgatct
      481 tgttgccgta agccccgatc gcctcagtgc ggtggtcgtc gccgacgatc agaagaacat
      541 tcggtcggcg atcagcggca ttggccgatt gaaacagacc gaccagcatg accaggcaag
      601 tgagtaatcg gttcatgatg gggctccctg tcaaaggtgc gagcaagagc cggagattcc
      661 ggcaagacta acgcccgagg ggcatccgcc gggccgcaag cagcatttga gcagtactat
      721 cggccgttcg caagggggcg gagccagcat cttccgactc cagagcttcc ggcgtataat
      781 agaatggtcc tcggaggtgt ccatgcgcga gattcacaag aaagtcatta cggacgatca
      841 aatgcgaccg gtggccgtgc agattgatta cgccgattgg gtcgagatcg agaggcagct
      901 cgctgaaagg caattgcctg tcgctgacgc tggtccgacc gatctatctt cctttgcagg
      961 gacactgagg tggagcgagg acggtgtcgc ctaccagcgt cgtgtgcgcg aggaatggga
     1021 ccggtgaccg tccttgacac caatattctg atctacctcc tcgaagggcg tcttcaggac
     1081 gagctaccgg ggtcgggtct atgcctgtcg gtcatcacgg aaatcgagct tctttcttac
     1141 agccgactga ctgaggacca gaaggccaga attcaggata tggtcacccg gttgaccgtt
     1201 attgggttga ctcctcaagt gaaggaagcg gccatacaac tccgcagagt caaccgcctt
     1261 acgattcctg atgcgattgt cgccgcaaca gccctttccc tgggtgcgga gcttcttacg
     1321 aatgacgaca agctcatgac cgttccgggg ctggcttgtc ggcggatgaa tgtcgcgccg
     1381 ccgtaacgga ctaatgcggc cgtttgaccc tcgcactttc atcaaccaat tatctggtgg
     1441 tatccagggt tgccgcacat cagcaaggtc gaaagactaa ggctggtctg ttcggacatg
     1501 ttgcttggtt ttgagcggct tctcttgatc caggtcggca gcgacggcgc ggcaaggggt
     1561 gctgccggat gaccgttttg ccgtcgtggt tggccgactg agaacctgcc gcgatggtgt
     1621 cggatgccgt ccacctcccg ctggtgatga attgtcgctc ccaatccttg cgccgatttt
     1681 gtacaatggc gcatagcgga tgaggtcctg gagcaccgac cgtttccaca caggactgaa
     1741 agcagacgaa tcaggaggcg aaggctggat gaggagcatg gcggcgttgt tgaccgtttt
     1801 gtggtcctgt gtctggctgc cggcggctct ggcccagacg ccgaacccct acgggatagc
     1861 cggttggggc aggctcagca agctcaaca
//
LOCUS       DAOTPW010000438         1841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_49331_length_1841_cov_4.081747, whole genome shotgun sequence.
ACCESSION   DAOTPW010000438 DAOTPW010000000
VERSION     DAOTPW010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49331_length_1841_cov_4.081747"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1841)
                     /locus_tag="P5316_21900"
     CDS             complement(<1..>1841)
                     /locus_tag="P5316_21900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRR87669.1"
                     /translation="SVTESWQVTVYQPPPSDGSNVAQPWGTLHGNIFATQSSDDLSLL
                     FDSRWSNGARISWTYSATVDGLTGTTERGGITFDEDGNLYWKTSEGLLASLAPDKTLR
                     WKGNEAGTPVDLGQGDTTTPVVGDGGPTGRVYVLGDSGLYAFQKSDGAQLWTTALPDA
                     NFASTGNRLTPLLYEGRVYVVGAGETTKTVYEIDAATGTIIWAKQITVNLDTGWGDAK
                     GAMTLVPNALGAGIHGLYFNADGSGDGTDVYCIAVNTNTYSGSLQWMADGGKAARSHV
                     IYSATTGRLYTATWGDDGKQFYSFSPTTGLLFGNNSPENSGHGYNDFGCLDFNGTDVI
                     AAGFGGNVIRYHDTGDGSTISTFYPTSSNYGEFRMYGGLYKDADGDSIAVMATRSTEG
                     PNARVLAFNATTEPQVSCASYEDAGVYIDNLKITHGATGVENTRLDVAGFEGYALGDL
                     PGQANPGGTGTATWEDDTGGGASAVGKVQVVNNLPAGGSGKGILIDAAGGCGGWQGVF
                     ANLGGALTDTVVIVSWDQYRGDLTDNEWFAENIDYSGWWAMAWDSSTPVGSISPRNFD
                     AWTPLAAGAWQHIEYRFDFGASTPTVTVSVNGVQAGVNLAAGETSIR"
BASE COUNT          314 a          612 c          554 g          361 t
ORIGIN      
        1 cgcggatgct cgtttcacct gcagccaggt tgacacccgc ctggacgccg tttacagaga
       61 cagtcaccgt tggagtggac gctccaaagt cgaaccggta ctcgatgtgc tgccaggcac
      121 ccgcggccag gggggtccaa gcgtcaaagt tgcgagggga gatgctcccg accggggtcg
      181 acgagtccca ggccatggcc caccagccgc tataatcgat gttctcggca aaccattcgt
      241 tgtcggtcag gtcgccgcga tactggtccc agcttacgat cacgaccgtg tcagtaagag
      301 cgccgccgag attggcaaag acgccctgcc aaccgccgca tccgcccgcc gcatcgatca
      361 agattccctt gccgctgccg cctgcaggca ggttgttcac cacttggacc ttgcccacgg
      421 ccgacgcacc gccgccggta tcgtcctccc aggtcgcggt accggtaccg ccggggttgg
      481 cttggccggg cagatcgccg agcgcgtagc cctcaaaccc cgcgacgtcc agccgcgtgt
      541 tctccacgcc cgtggccccg tgcgtgatct tcaagttgtc gatgtagacg cctgcatcct
      601 cgtaggaggc acagcttacc tggggctctg tggtggcgtt aaacgccaga accctggcgt
      661 tcggcccttc cgtgctacgg gtggccatca cggcgatgct gtccccgtcc gcgtccttgt
      721 acagcccgcc gtacatacgg aactcgccgt aattgctgct ggtcgggtag aacgtgctga
      781 tggtcgagcc gtcaccggta tcgtgataac ggatcacgtt gccgccgaag cccgccgcga
      841 tcacgtctgt tccgttaaag tccagacagc cgaaatcgtt gtacccatgg ccgctgttct
      901 ccgggctgtt gttgccgaac agcaaacccg tggtcgggct gaagctgtag aactgcttgc
      961 cgtcatcgcc ccacgtcgca gtgtagagcc gccccgtggt agccgagtag atgacgtgac
     1021 tccgggcggc cttcccaccg tccgccatcc actgcagcga tccactgtaa gtgttggtgt
     1081 tgacggcaat gcagtagacg tcggtaccgt caccgctgcc atcggcattg aagtacaacc
     1141 cgtgaatgcc ggcccccagg gcgttgggca ccagggtcat cgcgcccttg gcgtcacccc
     1201 aaccggtatc caggttcacc gtgatctgtt tggcccagat gatcgtgccc gtcgcggcgt
     1261 cgatctcata aacggttttg gtggtttccc ccgcacccac gacgtatacc cgcccctcgt
     1321 aaaggagcgg cgtcaggcgg ttgccggtcg aggcgaagtt ggcatccggc aaggccgtgg
     1381 tccagagttg tgcgccatca gacttctgga aggcatacaa gcccgagtcg ccaaggacat
     1441 agacccgccc ggttgggccg ccgtcgccga ccaccggtgt tgtggtgtcg ccctgtccca
     1501 ggtcaaccgg cgtcccggcc tcgttgcctt tccagcgaag cgtcttgtcc ggtgccagcg
     1561 aggccagcag accttcactg gtcttccagt acaggttgcc gtcctcatca aacgtgatcc
     1621 cgccccgctc cgtcgtgccg gttaatccgt caacagtggc actgtaagtc cagcttatcc
     1681 tggcgccgtt cgaccagcgg ctgtcgaaaa gcagcgagag atcgtccgaa gactgggtcg
     1741 caaagatgtt cccgtgaagg gttccccacg gctgggcgac gttgctgccg tctgacggcg
     1801 gcggttggta gaccgtgacc tgccacgact cggtcacgct g
//
LOCUS       DAOTPW010000439         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_51919_length_1776_cov_4.833236, whole genome shotgun sequence.
ACCESSION   DAOTPW010000439 DAOTPW010000000
VERSION     DAOTPW010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51919_length_1776_cov_4.833236"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..163)
                     /locus_tag="P5316_21905"
     CDS             complement(26..163)
                     /locus_tag="P5316_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87670.1"
                     /translation="MRTGTSKRWHSLALLACLASLAAYLACSRVVPQFWRQLIEAIRS
                     C"
     gene            complement(188..925)
                     /locus_tag="P5316_21910"
     CDS             complement(188..925)
                     /locus_tag="P5316_21910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR87671.1"
                     /translation="MPMTQQAAPDIPRCADEVRTVLEMMGDMRAYHRWLFDTAQPYLR
                     GRVCEVGGGTGNITQYLIGYEDVVVIEPEPLSRRLLEERFAGFSNVTIVGLPLERCPC
                     EHVVPGSFDSLLSVNVLEHIEDHLAAVRKMTTLVRPQGLVIAIVPALNALLGELDHAA
                     GHYRRYSRGSLTRLFEQAGLTVVRARYFNAIGALGWWVNSRIRGCSRMSQAYYQKCER
                     AVPLVRFFDHLCPIPLGQSLLIVGQRP"
     gene            complement(1215..1700)
                     /locus_tag="P5316_21915"
     CDS             complement(1215..1700)
                     /locus_tag="P5316_21915"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRR87672.1"
                     /translation="MAPEEIADILQATFGDAVGVTEVRSGHPSVSVAAHRWHEIALFL
                     RDDDRLRLNMLRCVSGVDLPEQNQIEIIYDLTSLRPPRVVGSYWTGDNEIAIRVRVPR
                     EGGRIPSAADVWPAADWHEREVFDMLGVRFDGHPSLREIMRPEDWKDHTLRKDTAFPE
                     G"
BASE COUNT          353 a          561 c          522 g          340 t
ORIGIN      
        1 gggatgttgc ccaccggtag ccatctcagc agctccggat cgcctcgatc aactgtcgcc
       61 agaactgcgg caccacgcgc gagcatgcca gatacgcggc caggcttgcc aggcacgcga
      121 gcaaggcaag agagtgccat cttttcgagg tccccgttcg cattcgatct tctcccgcaa
      181 tcactgctca aggacgctgt cccacaatga gcagcgattg tccgagcggt atggggcaca
      241 ggtggtcgaa gaacctcacc aggggcacgg ctcgttcgca tttctggtag tacgcctgcg
      301 acattcgaga gcatccccga atacggctgt tgacccacca gccgagagct ccgattgcgt
      361 tgaagtatcg tgctcgcacg acggtgaggc cggcttgctc gaagaggcgc gtcagcgacc
      421 cccgcgagta acggcggtag tggcctgccg catggtccag ttcgcccaga agagcattca
      481 aggccgggac gatcgcgatc accaatcctt gcggacgaac aagggtcgtc atcttccgta
      541 ccgccgcgag atgatcctcg atgtgctcca gcacattgac cgacaacagt gagtcaaacg
      601 agccggggac gacgtgctcg cagggacagc gctccagggg caatccgacg atggtcacgt
      661 tcgaaaagcc ggcgaaacgc tcttcaagca gacgacgact cagtggctcc ggctcgatca
      721 ctaccacgtc ttcatatccg atcagatact gtgtgatgtt gccggttccg ccgccaacct
      781 cgcaaacgcg cccccgaagg tatggctgtg cggtgtcgaa caaccatcgg tgataagcgc
      841 gcatgtcgcc catcatctca aggacggtcc ggacttcgtc ggcgcaacgc ggaatgtcag
      901 gcgccgcttg ctgtgtcatt ggcatgatgg ttgtcgattc tgcccaggct cttgtcgggt
      961 accatgttcc cgaccggcgg tattatcggc accggggaga acgctctgaa gatggaggaa
     1021 agtcgcaaag accgataatt cgacccaggt cgcgatgcgg cgtgggaatg ggtttgtatc
     1081 cgaagagatg cggcgttcct ggcctgaacg tcaacgcagg gtaaatgccg ggccgtgatg
     1141 gcggaacaac agtctcgcgt gggatgaata tcgcggtgtg ctcagaaccg ccgaatctgc
     1201 cgcccacgcg tcgatcagcc ctctggaaac gcggtgtcct ttcgcagcgt atggtccttc
     1261 caatcctccg gtcgcataat ctcccgcagg ctcgggtgcc cgtcgaacct gacccccaac
     1321 atgtcgaaga cttcgcgctc gtgccagtcc gccgccggcc agacgtcggc ggccgaaggt
     1381 atacggccgc cttcccgcgg aaccctgacc cggatggcaa tctcgttgtc gcccgtccag
     1441 tatgatccca cgactcgcgg tggtcgaagc gacgtcaggt cgtagataat ctcaatctga
     1501 ttctgctcag gtagatcaac accgctgacg catcgcagca tgttgaggcg taaccgatcg
     1561 tcatcgcgca agaacagtgc gatctcatgc catcggtgcg ccgccacact gaccgacgga
     1621 tggccgcttc tcacctcagt cacacccact gcgtctccaa acgttgcctg caggatgtcc
     1681 gcaatctctt cgggtgccat ggttcacacc agcgctctcc gcggccgggt cttcgctgcc
     1741 tcgggctcgg cggcgacgat ccgagcctta gtctgg
//
LOCUS       DAOTPW010000440         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_52285_length_1767_cov_4.356308, whole genome shotgun sequence.
ACCESSION   DAOTPW010000440 DAOTPW010000000
VERSION     DAOTPW010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52285_length_1767_cov_4.356308"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="P5316_21920"
     CDS             <1..273
                     /locus_tag="P5316_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87673.1"
                     /translation="ILEGIILSLIGQSLGLEIAPLRPVSFQNVTTREHGEKGLIVYEL
                     ELATKFNLKAASDETVTDLCAIGLSYCLKPGDETADAADEVHLAET"
     gene            284..472
                     /locus_tag="P5316_21925"
     CDS             284..472
                     /locus_tag="P5316_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87674.1"
                     /translation="MKVQAAPGTRCPMETNPRNYITDAEPVDVPDSPYYRRLIADGSL
                     AEPPAQEAKKKEVKADGK"
     gene            462..>1767
                     /locus_tag="P5316_21930"
     CDS             462..>1767
                     /locus_tag="P5316_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HRR87675.1"
                     /translation="MASKNISFDNIPSSIRKPGKYFEFNTKLAVRTLPQNQQNALIIG
                     QRTSAGTVQANILTPIFSDKDAEVYFGVGSMLHLMARAAIKANPYMQLTAIALDDAGA
                     GVAATGTVTISGPATAAGTFTLYVANQAITIAFASADSANTIAAALNTELAAHPDLPV
                     TATVASAVVTLTARNKGLCGNDIGLGYAVTYNAGVGATIVAMASGATNPTLTSALAAA
                     FAYQYDIIIFPYNSQADLTTLKTHLDSVSGPMEQRPGIGVYGMNGALGTCTTLSAQIN
                     HGRMLCAYHRYTSSTVRKSMPYEIAAAMGAVMAWEEDPARPLNTLALAGIAPAAINDR
                     FSRTEQENLLYNGVTPLEVGPGENVQIVRAISTYIKDANNIADVSLLDITTIRILDYV
                     RRACRERVALRFPREKLSSKTPPRVKSELLDVLLKLQDLEIVE"
BASE COUNT          391 a          548 c          533 g          295 t
ORIGIN      
        1 attttggagg gcattattct gagcctgatc gggcagagcc tgggcctgga gatcgccccg
       61 ctccggccag tgtccttcca gaacgtcacg accagggaac acggcgaaaa ggggctcatc
      121 gtctacgagc tggagctggc cacgaagttc aatctcaaag cggcgagcga cgagacggtg
      181 acggatcttt gcgcaatcgg gctcagttac tgcctgaagc ctggggacga gacagcggac
      241 gcggccgatg aagtgcatct cgccgaaaca tagaggaggc catatgaaag tacaggctgc
      301 tccgggcacg cgatgcccga tggagacgaa cccgcggaac tacatcacgg acgcggagcc
      361 ggtagacgta cctgacagcc cctactatag gcggctgatc gctgacggat cgctggcaga
      421 gccgccggca caggaggcga agaagaagga ggtaaaagcc gatggcaagt aagaatatca
      481 gtttcgacaa tatacccagc agcatccgca agccgggaaa gtatttcgaa ttcaacacaa
      541 aacttgcggt cagaacgttg ccgcagaatc agcagaatgc cctgatcatc ggccagagga
      601 catcggcagg cactgtgcag gccaacatac tcacgccgat cttttcggat aaggatgcgg
      661 aggtctactt cggcgtcggg tccatgctgc acctcatggc acgcgcggcg atcaaggcga
      721 atccctacat gcaactgacg gccattgcgc tcgatgacgc gggcgccggc gttgcggcca
      781 cgggaacggt tacgatcagc ggtccggcga cggcggccgg gacgttcact ctctacgtgg
      841 cgaaccaggc gatcaccatc gcattcgcga gtgccgattc cgcaaacacc attgcggccg
      901 ccctcaatac ggagctggct gcccaccccg atctgccggt caccgcgact gtggccagcg
      961 ccgtagtgac actgaccgcc aggaacaagg gcctctgcgg caacgatatc ggccttggtt
     1021 atgccgtgac gtacaatgcc ggcgtgggcg ccaccatcgt cgctatggcc agcggcgcga
     1081 ccaacccgac gctgacctcc gccctggctg cggcttttgc ctaccagtac gacatcatca
     1141 tcttcccgta caacagccag gcggacctaa cgacactgaa aacgcatctc gattcggtat
     1201 ccggtccgat ggagcaacgg cctggtatcg gcgtgtatgg catgaatggc gccctgggca
     1261 cctgcacgac gctctctgcc cagatcaatc acggaaggat gctctgcgcg taccatcgct
     1321 atacgtccag caccgtgaga aagtcgatgc cctacgagat cgccgccgcg atgggggccg
     1381 tcatggcatg ggaggaggat cccgcccggc cgctcaacac gctggccctg gccgggatcg
     1441 ctccggctgc catcaacgat cgcttctccc ggaccgagca ggagaacctg ctctataacg
     1501 gggtcacgcc gctggaagtc ggcccagggg aaaacgtcca gattgtccgg gccatcagca
     1561 cctacatcaa ggatgcaaac aatatcgccg atgtttcgct tctcgacatc acgacgatcc
     1621 ggatcctgga ctatgtccgc cgggcctgcc gcgagcgggt ggcactccgg ttccccaggg
     1681 agaagctatc ctcgaagact ccgccgaggg tgaaaagcga actcctcgac gtgctcctga
     1741 agctccagga cctggagatc gtcgagg
//
LOCUS       DAOTPW010000441         1643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_58100_length_1643_cov_5.126574, whole genome shotgun sequence.
ACCESSION   DAOTPW010000441 DAOTPW010000000
VERSION     DAOTPW010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58100_length_1643_cov_5.126574"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="P5316_21935"
     CDS             <1..610
                     /locus_tag="P5316_21935"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRR87676.1"
                     /translation="PAGLPLDPAAIDAELARRFRKPIDPGPREKWARRLPVPFRSQKW
                     EAKEVRGSVCSPTSVAMVLEYYGVRLPTSRVYELVYDAEFKLYGNWARAVQTACHFGV
                     PGYIERFGDWDAVKRHIVAGRPVIASIRVDKGQLRNAPYRESNGHLLVITGFDGKGGV
                     CINDPAGGTPEQGVITHPVEDVERIWFQRGGVGYVLLGRPPE"
     gene            636..>1643
                     /locus_tag="P5316_21940"
     CDS             636..>1643
                     /locus_tag="P5316_21940"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRR87677.1"
                     /translation="MSNPSCLTKEQVFQALKSVRDPELGQDIVSLGFVKNVETGDGRV
                     CVEVELTTPACPMRETIRHDIETAVCDLGGVTEVDVEFSARVRSSLPEKCQLPGVRNI
                     VAVGAGKGGVGKSTLSVLLSIGLARAGARVGLLDADVYGPSIPKMLGIEDARPGYEND
                     KVLPVEVLGIKAMSMGLLIPPAQAVIWRGPMIHNAIRQFLEQVEWGELDYLVVDLPPG
                     TGDVPLTLAQSIPLTGAVVVCTPQAVALADAARAARMYEALGVPVLGMIENMSYFIAP
                     DTGREYDLFGKGGAEVAARELGVPFLGAIPINVAIRVAGDEGTPDSLFRGDSQGVGDA
                     LK"
BASE COUNT          283 a          443 c          583 g          334 t
ORIGIN      
        1 gccggcgggg ctgccgctcg acccagccgc cattgacgcc gagctggccc gccggttccg
       61 caagccgatt gaccctggtc cccgtgagaa atgggcccgc cgattgccgg tgccgtttcg
      121 gagtcagaag tgggaggcca aggaggtccg cggaagcgtc tgcagcccga cgtcggtggc
      181 gatggttctc gaatactacg gagtaaggct gcccacttct cgcgtgtatg agctggtgta
      241 cgacgccgag ttcaaactct acggcaactg ggcccgcgcg gtgcagactg cctgccattt
      301 tggagtaccc ggttacatcg agcgtttcgg cgactgggac gcggtcaaac ggcatatcgt
      361 ggccggtcgc ccggtgattg ccagtattcg tgttgacaaa ggccaactgc gcaatgcccc
      421 gtatcgcgag tcgaacggtc atcttcttgt catcacgggt ttcgacggga agggcggcgt
      481 gtgtattaat gacccggccg gcgggactcc ggaacagggc gtgatcacgc atcccgttga
      541 ggacgttgag agaatctggt tccagcgggg cggagtgggc tacgtgttgc ttggccggcc
      601 gccggagtga cagcttctat cgcgaggtgc attccgtgag caatccatcc tgcctgacaa
      661 aagagcaggt gtttcaggcg ctgaagagtg ttcgagaccc cgaactgggt caggacatcg
      721 tttcgctcgg ttttgtcaag aatgtggaaa ctggcgacgg tcgtgtttgc gtggaggtcg
      781 aattgaccac cccggcatgt cccatgcgtg agaccattcg ccatgacatc gagacggccg
      841 tgtgtgacct gggcggcgtg acagaggtgg acgtggagtt ctccgctcgt gttcgcagca
      901 gtctgcccga gaagtgccag ttgcccggcg ttcggaatat cgtggctgtg ggggcgggca
      961 agggtggcgt cggcaagagc acgctgagcg tgttgctatc gatcggcctg gctcgcgcgg
     1021 gggcgagggt cgggttgctc gatgcagatg tttacggtcc ctcgattccc aagatgctgg
     1081 gcatcgagga cgcccggccg ggttacgaga acgacaaggt tttgccggtc gaagtcctgg
     1141 gcatcaaggc gatgagcatg ggcctgttga ttcccccggc gcaggcggtc atctggcgag
     1201 ggccgatgat tcacaatgcg atacggcaat tcctggagca ggtcgagtgg ggagaactgg
     1261 actacttggt ggtcgatctt ccgcctggca caggcgacgt gccgttgacg ttggcacaat
     1321 cgatcccgtt gacgggggcg gtcgtggtat gcacgcccca agcggtggct ctggccgacg
     1381 cggcccgggc cgcgcgaatg tacgaagcgc tgggggtgcc ggtgctcggg atgatcgaga
     1441 acatgagcta ctttatagcc cctgacacgg gccgtgaata cgaccttttc ggcaaggggg
     1501 gagcagaggt tgctgcccgg gagctgggtg tgcctttcct tggggcgatt ccgatcaatg
     1561 tggcgatccg cgtggcgggt gatgaaggga ctcccgactc gctttttcga ggcgattctc
     1621 agggcgtcgg cgatgccctg aag
//
LOCUS       DAOTPW010000442         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_59536_length_1617_cov_5.389885, whole genome shotgun sequence.
ACCESSION   DAOTPW010000442 DAOTPW010000000
VERSION     DAOTPW010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59536_length_1617_cov_5.389885"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            575..784
                     /locus_tag="P5316_21945"
     CDS             575..784
                     /locus_tag="P5316_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87678.1"
                     /translation="METGTTLESGVDPTATPHKWQRPSIGQMIAATFGYAHYEDDGSG
                     GEQLVLGWINEMVDQEACESAIESE"
     gene            794..1417
                     /locus_tag="P5316_21950"
     CDS             794..1417
                     /locus_tag="P5316_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87679.1"
                     /translation="MGAPGKSVVIEGGKRGVLLGGKSAVYNAEETCPACCIEFSRKWS
                     FTDQGFIDGGQGGAYRAYDDPDDVPASPWTVLNNGLGLRLDWEDDYNCRSHNPYTQNA
                     TATCEITVPKRMLMTVNWSGVGEVQAPGFDVMSLYVDGNLVGSAHAPGGSQGCSPMEP
                     VVSDPPPPQQVILEPGPHTLFISASTNDPLYHFDAWYQFALTFALAP"
     gene            1437..>1617
                     /locus_tag="P5316_21955"
     CDS             1437..>1617
                     /locus_tag="P5316_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87680.1"
                     /translation="MPDTLIPKAKKCGNCPPLVVPRRAYTPSRPSCIECVEKHLGAAY
                     VLLTEARDRPAPSGAR"
BASE COUNT          322 a          496 c          528 g          271 t
ORIGIN      
        1 agcggaaccc cgacatgcgc ttcctgttta ccagcggcta tgtggatgag aaatcgggtt
       61 ggacagcaat caagcagcgt ggctggaaat gcctcgtcaa gccttgtccc gccagcgaac
      121 tgctggccgc cgtggatgag gcgctggcac atgatgccga ggggtaatca gattatgagt
      181 gggaaagcaa caacgatgat gccagatgcg aaagagacgg tcgtacaggg acagtgttgc
      241 cgtatgctcc gcatgctctc cgactgtaac cggatcttgg tgcacagcac gaacgaaagc
      301 ggattgctcg gcgatctgtg ccgcctcttg ttagatgagg gaggatgcac ggccgcttgg
      361 gtcggcactg tacgggacga tgcggtggga accgtcagaa cggtcgtgca tgtcggcttc
      421 caaggaaaca taccggccac cttcgatttg atggacacgg aagatagcgc aacgtttgaa
      481 tcttgggtca gccaggtcat ccgcacgggc caaatgcgct gttgcgatga tctgaaccag
      541 atacccggcg acctggacct acgacgtcaa ggacgtggag accggaacca cgctggagag
      601 cggcgtcgat ccgaccgcga cgccgcacaa gtggcagcgg ccgtccatcg gccagatgat
      661 cgccgccacg ttcggctatg cccactacga ggacgacggt tccggcggcg agcagctcgt
      721 tctcggctgg atcaacgaga tggtggatca ggaagcctgc gagtcggcca tcgagtcgga
      781 gtgaggaggc ggcgtgggcg cacccggcaa atcggtggtg atcgagggcg gcaagcgtgg
      841 cgtgctgctg ggcggcaagt cggcggtgta caacgccgaa gagacctgtc cggcctgctg
      901 catcgagttc tcgcggaagt ggtcgttcac cgaccagggc ttcatcgacg gcggccaggg
      961 cggggcctac cgcgcctatg acgaccccga cgacgtgccc gccagcccgt ggacggtcct
     1021 caataatggc ctgggactgc ggctcgactg ggaggacgac tacaactgcc gatcccataa
     1081 cccctacacg cagaacgcga cggccacatg cgagatcacc gttcccaagc gcatgctcat
     1141 gacggtcaac tggtcgggcg tgggcgaggt gcaggccccc ggattcgatg tgatgagcct
     1201 ctatgtggac ggcaatctgg tgggatcggc ccacgctccg ggcgggagcc aaggctgctc
     1261 gcccatggaa ccggttgttt ccgacccgcc gccgccccag caggtgatcc ttgagccggg
     1321 accgcacaca ctcttcatca gcgcatcgac caacgatccg ctttaccact tcgacgcttg
     1381 gtatcagttc gcgctgacct tcgcgctcgc gccgtaacca ttggaggacc ccgaccatgc
     1441 ctgacaccct gattccgaaa gccaagaagt gcggcaactg cccgccgctg gtcgtgccgc
     1501 gccgggccta cacgccgagc cgaccgagct gtatcgagtg cgtcgaaaag catctcggcg
     1561 cggcctacgt gctcctgacc gaggcccgcg accggcctgc gccaagcgga gcgcggc
//
LOCUS       DAOTPW010000443         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_62626_length_1564_cov_5.834990, whole genome shotgun sequence.
ACCESSION   DAOTPW010000443 DAOTPW010000000
VERSION     DAOTPW010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62626_length_1564_cov_5.834990"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1187)
                     /locus_tag="P5316_21960"
     CDS             complement(171..1187)
                     /locus_tag="P5316_21960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR87681.1"
                     /translation="MYPYTKSTHSGAHLRLFASVLFALAISVAGCAGPKTGLDHGAFT
                     IVMLPDTQIYSKAYPDLFYAQTRWIRANRDRENIKCVTHVGDIVNDCVKDPEQWDVAD
                     KAMAVLDGVVPYGVAIGNHDYEGRSSAVAFVKYFGPDRYKGRPWYRGASENKLSSCQL
                     FSGGGVDFVILHLEIDVPDPTIEWAIDVLRMYPDRAAIVSTHAYLKGRDGVARNTKPA
                     YDKRGNSGEEVWDKLIRRNPQIFMVLCGHEGRTEEYHQISKNDAGNRVVEMLADYQKR
                     SNGGEGWLRLIRFVPSSRQIQVRTYSPALGQFETDSNSQFTVPWDLPDMCRRNSRLPL
                     AVGG"
     gene            complement(1209..>1564)
                     /locus_tag="P5316_21965"
     CDS             complement(1209..>1564)
                     /locus_tag="P5316_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87682.1"
                     /translation="KDGNAGEDIWQKFIRRQPQVFMVLCGHVGGLCEYRQTSRNDAGA
                     DVLEMLADYQKRENGGDGWLRLIRFVPLNREIQVRTYSPALNKFETDEDSQFVVPLVL
                     PDQCLPKPAAVALAG"
BASE COUNT          305 a          479 c          461 g          319 t
ORIGIN      
        1 tcaccgcatc atcaaaccgg cggatttaca gcatgttcag accggcgccg acacccattc
       61 acgcgttcga cggcgaaggc aagcctgatg ctgttttcag gagcccagcg gaccgtttgg
      121 tcaacacccg aaaggtgtgt cctggatagc agcagcgact tctgcagtgg tcaaccaccg
      181 acagccaacg gtagcctgct gttcctgcgg cacatgtccg gcagatccca cggaacggtg
      241 aactggctgt tcgaatcggt ctcaaactgg cctagcgcgg gcgagtaggt gcggacctgt
      301 atctggcggg aggatggtac gaatcggatc agtcggagcc agccttcgcc gccgttactt
      361 cgcttctgat agtcggccag catctccacg accctgttac cggcatcatt cttcgatatc
      421 tggtggtatt cctccgtgcg gccctcatga ccgcagagaa ccatgaagat ctgcggattg
      481 cgacggatca gtttatccca cacctcctcg cccgaattgc cgcgtttgtc atatgccggc
      541 ttggtatttc gtgcgacccc gtcgcgtccc ttgagatagg cgtgtgtcga gacgatggcg
      601 gcgcggtccg ggtacatcct caagacgtcg atcgcccatt cgatcgttgg atcaggaaca
      661 tcgatttcca ggtgaagaat cacgaaatcg acgccgccac ccgagaacaa ctggcaggag
      721 ctcagcttgt tctcggaggc gccacggtac cagggtcggc ccttatagcg atcgggcccg
      781 aagtatttga cgaacgcgac ggcgctcgac cggccttcgt agtcgtggtt gccgatggcc
      841 acgccgtagg gaaccacgcc atcgagtact gccatggcct tgtcggcgac gtcccattgc
      901 tcggggtctt tgacgcaatc gttgacgatg tcgccgacgt gggtgacaca cttgatgttc
      961 tcgcggtcgc gattcgccct gatccagcgc gtctgggcgt agaacaggtc aggataggcc
     1021 ttgctgtaga tctgcgtatc gggcagcatc acgatggtga acgcaccatg atcgaggccg
     1081 gtcttcggac cggcgcaacc ggcaaccgag atcgccagag caaacaggac agacgcgaac
     1141 agacgcaaat gcgccccgga atgcgtgctc ttcgtgtacg gatacatatg ttctcccttt
     1201 cgtcggcttc aaccggcaag cgcgacggca gccggtttgg gcaggcactg atccggcagc
     1261 accagcggca cgacaaactg actgtcctca tcggtttcaa acttgttcaa cgcgggcgag
     1321 tacgtccgca cctggatctc acggttgagc ggtacgaatc ggatgagccg cagccatccg
     1381 tcgccgccgt tctcgcgttt ctggtaatcg gcaagcatct cgaggacgtc ggcgccggca
     1441 tcattgcgcg aggtctgccg gtactcgcag aggccaccca cgtgcccgca cagcaccatg
     1501 aacacctgtg gttggcggcg aatgaacttc tgccagatgt cctcgccggc gttcccgtcc
     1561 ttgc
//
LOCUS       DAOTPW010000444         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2833
            NODE_65007_length_1525_cov_4.396599, whole genome shotgun sequence.
ACCESSION   DAOTPW010000444 DAOTPW010000000
VERSION     DAOTPW010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867857
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 19:37:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,396
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,326
            CDSs (with protein)               :: 4,326
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65007_length_1525_cov_4.396599"
                     /isolate="AMR_MDS_2833"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="P5316_21970"
     CDS             <1..566
                     /locus_tag="P5316_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87683.1"
                     /translation="DAAKAKQAYYDLMEKFNAPVYAELKRPDGPFWAVDFGKGDFTAF
                     GMGGVFWCNEKAEGYFGHEIYLLPGQNIPEHRHLPTQDADGKPIRCKIESWQVRHGYV
                     YGFSEVGEPNLDQFPEAKAMLSKLQSPYLKCVHVEKWEADGKVHKLAGPETWHFMQAG
                     KEGAIVTEYATFHDNAGLRFSVPGTAL"
     gene            610..918
                     /locus_tag="P5316_21975"
     CDS             610..918
                     /locus_tag="P5316_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR87684.1"
                     /translation="MTYHHTGIPVFDKLDGMIFIEPLKVWITEADKHPYKVEFLYFEP
                     DSPMAAAIQNETHVAYIVEDIEKALQGKSVLWPVTTFPTMKIAFVYDGGLPVEYVQMV
                     "
     gene            937..>1525
                     /locus_tag="P5316_21980"
     CDS             937..>1525
                     /locus_tag="P5316_21980"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HRR87685.1"
                     /translation="MKKFINDPANLTKELLEGLALCYKGKINLVNEKIVVRAMPKDES
                     KVAIVTLGGAGHEPALSGFVGEGMLDASVVGDIFAAPGAPKLLEALRMFKREAGIVLV
                     TLNHAGDRMSASKALRDAQKEGIKVREIVTHEDISAGIDVDPEEKRGLAGCIPLYKVI
                     GAAAEAGKSLDEVVEIGERFNRQMATLAVAMTGCTH"
BASE COUNT          338 a          396 c          512 g          279 t
ORIGIN      
        1 tcgatgcggc gaaggccaaa caggcgtatt acgatctgat ggaaaaattc aatgcgccgg
       61 tgtatgccga gctgaagcgt ccggacgggc cgttctgggc agtggatttc ggcaaggggg
      121 atttcacggc attcggcatg ggcggcgtgt tctggtgcaa tgaaaaggcc gagggatatt
      181 ttggccatga gatttacctg ttgcccggcc agaacattcc ggagcaccgg cacttgccga
      241 cccaggatgc cgacggcaag ccgatccgtt gcaagatcga gagctggcag gtgcgccacg
      301 gttacgtgta tgggttcagc gaggtgggcg agccgaatct cgatcagttc ccggaagcca
      361 aggccatgct gtccaagctg cagagcccgt acctcaagtg cgtgcatgtc gagaagtggg
      421 aagccgacgg caaggtgcac aagctggcgg gacccgagac ctggcatttc atgcaggccg
      481 gcaaagaggg ggccatcgtc accgagtatg ccaccttcca tgacaacgcg gggttgcgct
      541 tctcggttcc gggcaccgcg ctgtaacgtt tgaaacaggg aaaacaacga ccaataaggg
      601 agaaccaaga tgacgtacca tcacactggc attccggtat tcgacaagct ggacggcatg
      661 atcttcatcg agccgctgaa ggtgtggatc accgaagccg acaagcatcc gtacaaggtc
      721 gaattcctct atttcgagcc ggatagcccg atggcggccg cgatccagaa cgagacccat
      781 gtggcctaca ttgtcgagga tatcgaaaag gccttgcagg ggaagagtgt gctgtggccg
      841 gtcaccacgt tcccgacgat gaagatcgcg ttcgtgtatg acggcggcct gccggtcgag
      901 tatgtgcaaa tggtgtaagg gagctttgtt atggcgatga agaagtttat caatgacccg
      961 gcgaacctga ccaaggaact gctggaaggc ctggctctgt gttacaaagg caagatcaac
     1021 ctcgtgaacg agaagatcgt ggtgcgcgcc atgccgaagg acgagagcaa ggtcgcgatc
     1081 gtcacgctgg gcggcgcagg ccatgagccc gcgctcagcg ggtttgtggg cgaagggatg
     1141 ctggacgcga gcgtggtcgg cgatatcttt gccgcgccgg gcgcacccaa gctgctggag
     1201 gcgttgcgca tgttcaagcg cgaagccggc atcgtgctgg tcacgctcaa ccatgcgggc
     1261 gaccgtatga gtgcctccaa agcgttgcgt gatgcgcaaa aagagggcat caaggttcgc
     1321 gagatcgtca cgcatgagga catcagcgcc ggtattgatg ttgacccgga agagaagcgc
     1381 ggtctggcgg gctgtatccc gctttacaag gtgatcggcg cggcggccga agccggcaag
     1441 agcctcgatg aggtcgtgga gatcggcgag cgcttcaacc gtcagatggc gacgctggcg
     1501 gtggcgatga ccggttgcac gcatc
//
